{ "genome": { "genus": "Mycoplasmoides", "species": "pneumoniae", "strain": "16-734", "taxon": "Mycoplasmoides pneumoniae 16-734", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 818445, "gc": 0.39968354623707153, "n_ratio": 0.0, "n50": 818445, "n90": 818445, "coding_ratio": 0.796423705930148 }, "features": [ { "type": "oriC", "sequence": "contig_1", "start": 1, "stop": 630, "strand": "?", "product": "origin of replication", "nt": "TGTGCCCAACCGCGAAAAAGAAAGTGGTATATAGGAAAATGGAAAACAAATTTGTTTACCGTTTACTATTAAATACTAATCTTTTATATAGTATAGAGAAACTTTTTCTTTAACATAATATTATCTTAATATTATTTACCTACTAATAGCTTAATATTATTAGTATTTATTTAGTATTATGCTAATACTATGCAGATATTATCTTAATATTATCTATAGTATTAGGCTAATATTATTCTTAATATTTATTAAGGTACTAAAGCATTACCTATAGGTGATATTATGACAATACTAAAGTGGTTAGTATTATTAGGGTATTATTCAAAGTATTCTCCAACACTATTCCCTTAGCTCTAACTTAATATCCCTTTAATACCAAGATGGTTAAACAATGAAGAAAATTAGTTGTTTGTTCTGGCCCTTTACTATATATGTACATAGTACATATATAAGAAAGGCTACCATGACATTATATATAATAGGCATCCAGAAATAAAAGCCAAATTAATCTATAATTAAGTATTTATACTTAATTATAGTGATTTTATTAACAAAATTAGTGACATTTGGCCATCCATAATAAATAAAATCAAATAAACAAAACAAAAAGAAAAAAACTATAAAAAGGGC", "id": "GIHJHMOALN_1446" }, { "type": "cds", "sequence": "contig_1", "start": 631, "stop": 1773, "strand": "+", "frame": 1, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360", "KEGG:K02338", "RefSeq:WP_014325278.1", "SO:0001217", "UniParc:UPI0001DD643E", "UniRef:UniRef100_A0AAX0SSD5", "UniRef:UniRef50_P47247", "UniRef:UniRef90_Q50313" ], "nt": "ATGAAAGTTTTGATTAATAAGAATGAGTTGAACAAAATCCTCAAAAAACTCAACAATGTAATCGTATCTAACAATAAGATGAAACCATACCACTCTTATTTATTAATAGAGGCTACAGAAAAGGAAATTAACTTCTATGCTAACAACGAGTACTTTTCTGCTAAATGTACCTTAGCCGAAAACATTGATGTACTTGAAGAAGGTGAAGTAATTGTTAAAGGCAAAATCTTTAGCGAACTCATTAACGGAATTAAAGAAGACATCATTATTATTCAAGAGAAAGATCAAACTCTTTTAGTCAAAACAAAAAAAACAAACATTAACCTTAACACGATTGATAAGAAAGAATTCCCCAGAATCCGTTTCAACCAAAACGTTGATTTGAAGGAATTTGATGAACTTAAAATCCAACACAGCCTTTTAACTAAAGGACTTAAAAAGATTGCCCATGCTGTTTCTACATTTAGAGAATCCACTAGAAAATTCAACGGGGTTAACTTCAACGGTTCCAATGGTAAACAAATCTTTTTAGAGGCATCGGATTCTTATAAGCTCTCTGTTTATGAAATCAAACAAAAAACCGATCCATTTAATTTCATTGTCGAAACTAATCTTTTGAGCTTCATCAATTCTTTTAACCCTGAAGGTGGTGATTTAATCAGTATCTTCTTCCGCAAAGAACACAAGGATGATTTAAGTACCGAATTACTGATTAAGTTAGATAACTTCTTAATTAACTACACCTCAATTAACGAAAGCTTTCCGCGGGTAATGCAGTTGTTTGACTTTGAACCAGAAACCAAAGTAACCATTCAAAAAAACGAACTTAAAGATGCATTACAAAGAATCTTGACGTTAGCTCAAAACGAGCGCTTCTTTCTCTGTGACATGCAAGTAACCAACTCCCACCTCAAAATTAATTCCAACGTTCAAAACATTGGTGCATCCTTAGAAGAAGTTACTTGCCTTAAGTTCGAAGGTCACAAACTCAACATCGCTGTTAATGCGCTTTCCCTCTTGGAACACATTGACTCATTTGATACTGATGAAATTGAGCTTTACTTCCAGGGCAGTAACAAATACTTTTTAATTAGTTCGAACAACGAACCTGAACTTAAAGAAATCCTAGTTCCTTCCAAGTAA", "aa": "MKVLINKNELNKILKKLNNVIVSNNKMKPYHSYLLIEATEKEINFYANNEYFSAKCTLAENIDVLEEGEVIVKGKIFSELINGIKEDIIIIQEKDQTLLVKTKKTNINLNTIDKKEFPRIRFNQNVDLKEFDELKIQHSLLTKGLKKIAHAVSTFRESTRKFNGVNFNGSNGKQIFLEASDSYKLSVYEIKQKTDPFNFIVETNLLSFINSFNPEGGDLISIFFRKEHKDDLSTELLIKLDNFLINYTSINESFPRVMQLFDFEPETKVTIQKNELKDALQRILTLAQNERFFLCDMQVTNSHLKINSNVQNIGASLEEVTCLKFEGHKLNIAVNALSLLEHIDSFDTDEIELYFQGSNKYFLISSNNEPELKEILVPSK", "aa_hexdigest": "3a85738d3f3ec1acc1ab810cb8dc3a0f", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI0001DD643E", "ncbi_nrp_id": "WP_014325278.1", "uniref100_id": "UniRef100_A0AAX0SSD5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD643E", "RefSeq:WP_014325278.1", "UniRef:UniRef100_A0AAX0SSD5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SSD5", "uniref90_id": "UniRef90_Q50313" }, "psc": { "uniref90_id": "UniRef90_Q50313", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47247", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_P47247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47247" ], "product": "Beta sliding clamp" }, "genes": [ "dnaN" ], "id": "GIHJHMOALN_43", "locus": "GCA009939745_00001" }, { "type": "cds", "sequence": "contig_1", "start": 1777, "stop": 2022, "strand": "+", "frame": 1, "gene": null, "product": "Chaperone protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8W2", "UniRef:UniRef90_A0AB38W8W2" ], "nt": "ATGACGCTTTATGACTTACTAGAGCTGCCCCAAACTGCTACACTACAGGAAATCAAAACAGCTTACAAACGCTTAGCTAAACGTTATCACCCTGACATCAATAAACAAGGGGCAGATACCTTTGTCAAGATTAATAACGCCTATGCCGTTTTAAGCGATACCACCCAAAAAGCGGAGTATGACGCTATGCTACGCTTTAGTGAATTTGAAGACCGAGTTAAACGCCTGGACTTCTCGATTAAGTGA", "aa": "MTLYDLLELPQTATLQEIKTAYKRLAKRYHPDINKQGADTFVKINNAYAVLSDTTQKAEYDAMLRFSEFEDRVKRLDFSIK", "aa_hexdigest": "ada1fc6b9036bcb5b7b7243b6261452f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1777, "stop": 2245, "observations": { "insertion": [ 2179 ], "mutation": [ 2020 ], "stop": [ 2020 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8W2", "query_cov": 1.0, "subject_cov": 0.5225806451612903, "identity": 1.0, "score": 161.0, "evalue": 1.79e-49, "gene_start": 1, "gene_end": 81, "reference_start": 1, "reference_end": 81, "reference_sequence": "MTLYDLLELPQTATLQEIKTAYKRLAKRYHPDINKQGADTFVKINNAYAVLSDTTQKAEYDAMLRFSEFEDRVKRLDFSIKWHEQFMEELQFHHNWDFDFIRNREYTQPTPTNNKYSSFLDKDVSLPFYQLYSKASWTLTWKTHFLDVIRSSKPS" }, "paralog": false, "identity": 0.9870967741935484, "score": 309.0, "evalue": 5.68e-110, "description": "Internal stop codon at 2020. Frameshift due to insertion around 2179. Nonsense mutation around 2020.", "psc": { "uniref90_id": "UniRef90_A0AB38W8W2", "product": "Chaperone protein", "uniref50_id": "UniRef50_A0AB38W8W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8W2", "UniRef:UniRef50_A0AB38W8W2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8W2" ], "product": "Chaperone protein" } }, "id": "GIHJHMOALN_44", "locus": "GCA009939745_00002" }, { "type": "cds", "sequence": "contig_1", "start": 2035, "stop": 2298, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGGAATTACAGTTCCACCACAACTGGGACTTTGACTTTATCCGCAACAGGGAATACACCCAGCCAACCCCTACCAACAATAAGTATTCTTCCTTTTTAGACAAGGATGTATCCTTGGCCTTTTACCAACTTTACTCCAAGGGCAAGTTGGACTTTGACTTGGAAGACACACTTCTTAGACGTCATTCGATCAAGCAAGCCTTCTTAAAGGGCAAGAAGCTTAACGATGTTTTAAAAGAGCAGTACAACTATTTAGGTTGA", "aa": "MEELQFHHNWDFDFIRNREYTQPTPTNNKYSSFLDKDVSLAFYQLYSKGKLDFDLEDTLLRRHSIKQAFLKGKKLNDVLKEQYNYLG", "aa_hexdigest": "1884f69e138bdc17e4d04b1d4c8b15b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10555.729799999992, "isoelectric_point": 6.824566841125488 }, "id": "GIHJHMOALN_45", "locus": "GCA009939745_00003" }, { "type": "cds", "sequence": "contig_1", "start": 2356, "stop": 2469, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGTGAAGGTGGGGTGGTCAACCTCCCTTTAAAGATCAAAGTCATTTCCAATAACTACCCTGGACAAATGTGGTACGAACTCAACAAGAACTATTCCTTCCGGCTTCTTTGA", "aa": "MREGGVVNLPLKIKVISNNYPGQMWYELNKNYSFRLL", "aa_hexdigest": "a612c54cf5a52c79fd9448aaf9c7bdd7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4386.1042, "isoelectric_point": 9.696383476257324 }, "id": "GIHJHMOALN_46", "locus": "GCA009939745_00004" }, { "type": "cds", "sequence": "contig_1", "start": 2793, "stop": 4745, "strand": "+", "frame": 3, "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872", "RefSeq:WP_010874360.1", "SO:0001217", "UniParc:UPI000012BECC", "UniRef:UniRef100_P22447", "UniRef:UniRef50_P22447", "UniRef:UniRef90_P22447" ], "nt": "ATGGAAGACAATAACAAAACGCAAGCTTACGATTCCAGTAGCATTAAGATTCTTGAAGGCTTAGAGGCCGTGCGTAAGCGCCCCGGCATGTACATCGGTTCCACCGGCGAAGAAGGGTTGCACCACATGATCTGGGAAATCATTGATAACTCAATTGACGAGGCCATGGGTGGCTTTGCGAGTACTGTCAAACTAACCCTTAAAGACAACTTTGTCACCATTGTGGAGGATGATGGCCGGGGCATTCCCGTTGACATCCATCCTAAGACCAACCGCTCCACCGTTGAAACAGTGTTTACGGTACTCCACGCTGGGGGTAAGTTTGACAACGATAGCTATAAGGTGTCAGGGGGACTCCACGGTGTGGGTGCCTCGGTTGTTAATGCTTTAAGTTCCTCCTTTAAGGTATGGGTAGCCAGGGAGCACCAACAGTACTTCCTAGCGTTCCACAACGGTGGGGAAGTGATTGGTGATCTTGTCAACGAAGGTAAGTGTGACAAGGAACACGGTACCAAGGTTGAGTTTGTCCCGGACTTTACCGTGATGGAAAAGAGTGATTACAAACAAACGGTAATTGCTAGTAGGTTGCAACAATTAGCCTTTCTCAACAAAGGGATCCAAATTGACTTTGTTGATGAACGCCGTCAAAATCCGCAAAGCTTTTCTTGGAAGTATGATGGTGGCTTAGTCCAATACATCCACCACCTCAACAACGAAAAGGAACCTTTATTTGAGGACATTATCTTTGGTGAAAAAACCGATACTGTTAAATCAGTTAGCCGTGATGAGAGCTACACAATTAAGGTGGAAGTGGCCTTTCAGTACAACAAGACGTATAACCAATCAATCTTTAGTTTTTGTAACAACATTAATACCACTGAAGGCGGTACCCACGTTGAAGGCTTTCGCAATGCCTTAGTGAAGATCATTAACCGCTTTGCTGTCGAAAACAAGTTCTTAAAGGAAACGGATGAAAAGATTACCCGCGATGACATCTGTGAAGGGTTAACGGCCATTATCTCAATTAAGCACCCCAACCCCCAGTATGAGGGTCAAACCAAAAAGAAATTGGGTAACACCGAAGTGCGTCCTCTAGTTAACAGTATTGTTAGTGAAATCTTTGAGCGCTTTATGTTGGAAAACCCCCAGGAAGCCAACGCCATTATTCGCAAAACGCTGTTAGCGCAAGAAGCACGGCGCCGTAGTCAGGAAGCACGGGAACTGACCCGCAGGAAATCTCCCTTTGACAGTGGTTCACTCCCGGGTAAGTTAGCAGACTGTACCACCCGTGATCCCTCAATTAGTGAACTGTACATTGTCGAGGGAGATAGCGCTGGTGGTACGGCTAAAACGGGGCGGGACCGTTACTTCCAAGCGATCCTACCATTAAGGGGTAAGATCCTCAATGTGGAAAAGTCCCACTTTGAACAAATCTTTAATAATGTGGAAATCTCGGCTTTAGTCATGGCCGTTGGCTGTGGCATTAAACCAGACTTCGAGTTGGAAAAACTGCGGTATAACAAGATCATTATCATGACCGATGCTGATGTCGATGGGGCGCACATCCGTACCCTCCTTTTAACCTTCTTCTTTCGCTTTATGTATCCCTTGGTGGAACAGGGCAACATTTACATTGCCCAACCCCCACTGTATAAGGTCTCTTACTCTAATAAGGACTTATACATGCAAACCGATGTCCAACTAGAGGAGTGGAAGCAACAACACCCTAATCTGAAATACAACTTACAACGCTACAAGGGTTTAGGGGAAATGGATGCCATCCAACTGTGGGAAACCACGATGGATCCCAAGGTGCGTACCTTACTAAAGGTAACCGTTGAAGATGCTTCAATTGCTGATAAAGCCTTCTCCTTGTTAATGGGCGATGAAGTTCCCCCAAGACGGGAATTCATCGAACAAAACGCACGTAACGTAAAGAACATTGATATCTAA", "aa": "MEDNNKTQAYDSSSIKILEGLEAVRKRPGMYIGSTGEEGLHHMIWEIIDNSIDEAMGGFASTVKLTLKDNFVTIVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKVWVAREHQQYFLAFHNGGEVIGDLVNEGKCDKEHGTKVEFVPDFTVMEKSDYKQTVIASRLQQLAFLNKGIQIDFVDERRQNPQSFSWKYDGGLVQYIHHLNNEKEPLFEDIIFGEKTDTVKSVSRDESYTIKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFLKETDEKITRDDICEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSIVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSHFEQIFNNVEISALVMAVGCGIKPDFELEKLRYNKIIIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIYIAQPPLYKVSYSNKDLYMQTDVQLEEWKQQHPNLKYNLQRYKGLGEMDAIQLWETTMDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEQNARNVKNIDI", "aa_hexdigest": "7fbdb582d3430eefa7a01fa4d02c95ce", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012BECC", "ncbi_nrp_id": "WP_010874360.1", "uniref100_id": "UniRef100_P22447", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012BECC", "RefSeq:WP_010874360.1", "UniRef:UniRef100_P22447" ] }, "ips": { "uniref100_id": "UniRef100_P22447", "uniref90_id": "UniRef90_P22447" }, "psc": { "uniref90_id": "UniRef90_P22447", "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P22447", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P22447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P22447" ], "product": "DNA gyrase subunit B" }, "genes": [ "gyrB" ], "id": "GIHJHMOALN_47", "locus": "GCA009939745_00005" }, { "type": "cds", "sequence": "contig_1", "start": 4745, "stop": 7264, "strand": "+", "frame": 2, "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335", "KEGG:K02469", "RefSeq:WP_014325279.1", "SO:0001217", "UniParc:UPI0002386BEA", "UniRef:UniRef100_A0AB33HM03", "UniRef:UniRef50_P47719", "UniRef:UniRef90_P22446" ], "nt": "ATGGCAAAGCAACAAGATCAAATTGACAAAATTCGTCAGGAATTAGCACAATCAGCAATTAAAAACATCTCCCTCTCCAGTGAACTAGAACGTTCCTTCATGGAGTATGCGATGTCGGTGATAGTGGCTCGCGCGCTCCCAGATGCGAGGGATGGACTTAAACCTGTGCACCGCCGTGTGCTCTATGGTGCTTACACTGGGGGCATGCACCACGATCGCCCCTTTAAAAAGTCAGCACGGATTGTCGGTGATGTAATGAGTAAGTTCCACCCCCACGGGGACATGGCCATTTACGACACAATGTCGCGGATGGCGCAGGACTTTTCTTTGCGTTACCTCTTAATTGACGGACATGGTAACTTTGGCTCGATTGATGGCGATCGTCCCGCAGCACAACGTTATACGGAAGCACGGTTGTCGAAATTAGCGGGGGAACTGTTACGTGGCATTGACAAGGACACGGTCGACTTTGTCGCTAACTATGATGGGGAAGAACAAGAACCAACGGTTTTACCAGCTGCCTTTCCTAATTTATTAGCTAATGGTTCGAGTGGGATTGCGGTAGGGATGTCCACTTCCATTCCGTCTCACAACTTATCGGAGTTAATCCAGGGCTTAATCCTTTTAATTGACAACCCTGACTGCACCATTAATGATTTATTAGGTGTCATTAAAGGACCGGACTTTCCCACCGGAGCCAACATTATTTACACCAAGGGGATTGAAAGCTACTTTGAAACTGGGAAGGGTAATGTGGTCATTCGTTCTAAAGTCAGCATTGAACAACTACCAACCCGGGCTGCTTTAGTAGTCACGGAAATTCCCTACATGGTCAACAAGACCAGTTTAATTGAAAAGATTGTCGAGTTAGTCAAGGCTGAGGAAATCACCGGGATTGCCGATATCCGTGATGAGTCATCCAGGGAAGGAATTCGCTTGGTGATTGAAGTGAAGCGCGACACCGTTCCGGAAGTCTTATTAAACCAACTGTTCAAATCAACCCGTTTACAGGTGCGGTTCCCCGTGAACATGTTGGCACTGGTTAAGGGTGCACCTAAATTGCTCAACATGAAGCAAGCGTTAACGGTTTACCTCGAGCACCAACTCGATGTTCTGATTCGCAAAACCCAGTTTAACCTCAAGAAGTACCAGGAGCGCTTTCATATCTTAAGTGGCCTTTTAATTGCGGCGTTGAACATTGATGAGGTAATTGCGATCATTAAAAAGTCCGCTAATAACCAGGTAGCAATGGAAGCACTCCACGAACGCTTCGGGTTAGATGAAATCCAAGCACGGGCGGTTCTGGACATGCGTTTACGTAGCTTAAGTGTCTTGGAAGTCAATAAACTACAAACCGAACAACAAGAGTTGAAAGCTTTAATTGAGTTCTGTCAACAGGTGTTAGCTGACAAACAACTGCAACTCAAACTAATTAAGGAGCAGTTAACCAAGATTAACGAACAGTTTGGCGATCCACGCCGTAGTGAAATCCTGTACGGTATTAGTGAAGACATTGATGATGAGGACTTAATTACCCAGGAAAACGTGGTAATCACGATGTCCACTAACGGTTATCTCAAACGGATTGGGGTAGACTCTTATAACCTGCAACACCGTGGTGGGGTGGGTGTTAAGGGTTTAACTACCTACACCGATGACAGTATTAGCCAGCTGTTAGTCTGCTCGACCCACTCGGACCTCTTATTCTTTACGGACAAGGGAAAGGTCTACCGCATCCGGGCCCACCAAATTCCCCCGGGTTTTCGTACTAACAAGGGGATTCCGGCGGTTAACCTGATTAAGATCGACAAGGACGAGAAGATCTGTGCCTTAATTTCGGTGAATGATTACCAAAATGGTTACTTCTTCTTTTGTACCAAGAACGGTACCATTAAGCGCACCAGTCTAAGTGAGTTTGCCAACATCTTGAGTATTGGTAAACGCGCCATCCTCTTTAAGGAAAACGATGTTTTGTTCTCGGTTATTCGCACCAGTGGTCAAGATGACATCTTTATCGGTTCGACCGCGGGGTTTGTCGTGCGTTTCCACGAAGACACCGTCCGTCCGCTGTCCCGCGCTGCCATGGGGGTCTTGGGGATTAATCTCAACCAGGGCGAATTTGTCAATGGTCTGTCCACTTCCAGTAACGGGAGTTTATTACTCTCTGTGGGTCAAAATGGCATTGGTAAGCTCACCAGTATTGACAAATATCGTTTAACCAAGCGGAATGCTAAGGGAGTTAAAACACTACGAGTAACTGCTAAAACTGGTCCGGTTGTGACCACTACGACCGTCTTTGGTAACGAAGATTTGTTAATGATTTCTTCAGCTGGTAAGATTGTCCGGATTAGTCTGGAACAACTTTCAGAACAGGGAAAAAACACTTCTGGTGTTAAACTAATCAAACTAAAAGAGAAGGAACGCTTAGAGACTGTTACTATCTTTAAAAAGGAAGAAGCCATTAAAACCACAACAGCAACCGAGACAGACGATGTTGGATCGAAACAAATTACGCAATAA", "aa": "MAKQQDQIDKIRQELAQSAIKNISLSSELERSFMEYAMSVIVARALPDARDGLKPVHRRVLYGAYTGGMHHDRPFKKSARIVGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGNFGSIDGDRPAAQRYTEARLSKLAGELLRGIDKDTVDFVANYDGEEQEPTVLPAAFPNLLANGSSGIAVGMSTSIPSHNLSELIQGLILLIDNPDCTINDLLGVIKGPDFPTGANIIYTKGIESYFETGKGNVVIRSKVSIEQLPTRAALVVTEIPYMVNKTSLIEKIVELVKAEEITGIADIRDESSREGIRLVIEVKRDTVPEVLLNQLFKSTRLQVRFPVNMLALVKGAPKLLNMKQALTVYLEHQLDVLIRKTQFNLKKYQERFHILSGLLIAALNIDEVIAIIKKSANNQVAMEALHERFGLDEIQARAVLDMRLRSLSVLEVNKLQTEQQELKALIEFCQQVLADKQLQLKLIKEQLTKINEQFGDPRRSEILYGISEDIDDEDLITQENVVITMSTNGYLKRIGVDSYNLQHRGGVGVKGLTTYTDDSISQLLVCSTHSDLLFFTDKGKVYRIRAHQIPPGFRTNKGIPAVNLIKIDKDEKICALISVNDYQNGYFFFCTKNGTIKRTSLSEFANILSIGKRAILFKENDVLFSVIRTSGQDDIFIGSTAGFVVRFHEDTVRPLSRAAMGVLGINLNQGEFVNGLSTSSNGSLLLSVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTAKTGPVVTTTTVFGNEDLLMISSAGKIVRISLEQLSEQGKNTSGVKLIKLKEKERLETVTIFKKEEAIKTTTATETDDVGSKQITQ", "aa_hexdigest": "1780d50d71b148647463a984d231a537", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386BEA", "ncbi_nrp_id": "WP_014325279.1", "uniref100_id": "UniRef100_A0AB33HM03", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386BEA", "RefSeq:WP_014325279.1", "UniRef:UniRef100_A0AB33HM03" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HM03", "uniref90_id": "UniRef90_P22446" }, "psc": { "uniref90_id": "UniRef90_P22446", "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47719", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47719", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47719" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "GIHJHMOALN_48", "locus": "GCA009939745_00006" }, { "type": "cds", "sequence": "contig_1", "start": 7236, "stop": 7799, "strand": "+", "frame": 3, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "COG:COG0172", "COG:J", "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_P47251", "UniRef:UniRef90_P75107" ], "nt": "ATGTTGGATCGAAACAAATTACGCAATAACCTTGACTTCTTTAAAAAGAAGTTAGTTGAACGGGGTGTAAGTGAAAGCCAGTTCGAGGCGTACGTGCAAGCTGATAAAGCAATGCGCAAACTGTTGCACCAAATTGAACTGGCCAACCAAAAGCAAACCCTGTTAGCGCAACAGGTTGCCAAGAAGAAGGGTGATCTGAAGTTATTAAAGGAATCCAAGGAGTTAAAGCAAAAACTAGAACAGCTCAACATCGCTTTTAAGGAAGCGGAAACCTTAAGCCAAGAGTTACTGTTAAACCTCCCTAACATTGCTGATGAATCAGTACCAGTTGGACGGGATGAAACTGCTAACTTAGAACTGCTCAAAGAAGGGCGTAAACCGGTGTTTGACTTTACTCCCTTACCCCACTGGGAGTTGTGTGAACGCTTACAGTTAGTTGCCTTTGATAAGGCCACTAAGCTAACGGGTGCACGGTTTGTCGCTTACACTGATAAAGCAGCGAAACTGCTCCGCGCCATTGCTAGCTTAATGATTGACTTCAACAAAAACAAATACCAGGAGTGA", "aa": "MLDRNKLRNNLDFFKKKLVERGVSESQFEAYVQADKAMRKLLHQIELANQKQTLLAQQVAKKKGDLKLLKESKELKQKLEQLNIAFKEAETLSQELLLNLPNIADESVPVGRDETANLELLKEGRKPVFDFTPLPHWELCERLQLVAFDKATKLTGARFVAYTDKAAKLLRAIASLMIDFNKNKYQE", "aa_hexdigest": "b50e5b6977994833fc6355dd7985b4c9", "start_type": "ATG", "rbs_motif": null, "genes": [ "serS" ], "truncated": "3-prime", "pseudogene": { "start": 7236, "stop": 8102, "observations": { "mutation": [ 7797 ], "stop": [ 7797 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75107", "query_cov": 1.0, "subject_cov": 0.4452380952380952, "identity": 0.9890000000000001, "score": 345.0, "evalue": 3.23e-116, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MLDRNKLRNNLDFFKKKLVERGVSESQFEAYVQADKAMRKLLHQIELANQKQTLLAQQVAKKKGDPKLLKESKELKQKLEQLNIAFKEAETLSQELLLNLPNIADESVPVGRDETANLELLKEGRKPVFDFTPLPHWELCERLQLVAFDKATKLTGARFVAYTDKAAKLLRAIASLMIDLNKNKYQEWNVPVIVNETSLTGTGQLPKFKDDVFKLENTRYYLSPTLEVQLANLHANEIFTEGELPKYYTATGVNFRQEAGSAGKQTKGTIRLHQFQKVELVKFCKPSEAIHELEEMTRDAEQILLELKIPFRRLLLCSGDMGFSAQKTYDLEVWMAGCNEYREVSSCSSCGDFQARRAMIRYKDLTTGKNTYVATLNGTALAIDRIFAAILEHYQTKAGEVMIPQALLKYLDFDKITKPK" }, "paralog": false, "identity": 0.9895833333333334, "score": 556.0, "evalue": 6.32e-202, "description": "Internal stop codon at 7797. Nonsense mutation around 7797.", "psc": { "uniref90_id": "UniRef90_P75107", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_P47251", "cog_id": "COG0172", "cog_category": "J", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75107", "EC:6.1.1.11", "UniRef:UniRef50_P47251", "COG:COG0172", "COG:J", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ] }, "pscc": { "uniref50_id": "UniRef50_P47251", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47251" ], "product": "Serine--tRNA ligase" } }, "id": "GIHJHMOALN_49", "locus": "GCA009939745_00007" }, { "type": "cds", "sequence": "contig_1", "start": 7803, "stop": 8498, "strand": "+", "frame": 3, "gene": null, "product": "Serine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A540V7F7" ], "nt": "GTGCCGGTCATTGTGAACGAGACTAGTCTTACTGGTACGGGTCAGCTACCCAAATTTAAGGATGATGTCTTTAAGTTAGAAAACACGCGTTACTACCTCTCCCCGACCCTAGAGGTGCAACTAGCCAACCTCCATGCCAACGAAATCTTCACGGAGGGTGAACTCCCCAAATACTATACCGCTACTGGGGTGAACTTTCGCCAGGAAGCGGGCAGTGCTGGTAAACAAACTAAGGGTACGATTCGCCTACACCAGTTCCAAAAGGTAGAACTAGTGAAGTTTTGTAAACCAAGTGAAGCGATTCACGAACTGGAAGAAATGACCCGGGACGCTGAGCAAATTTTGTTAGAACTCAAAATTCCCTTTCGCCGTTTGTTACTCTGTAGCGGTGACATGGGTTTTAGTGCCCAGAAAACCTATGACTTGGAAGTGTGGATGGCGGGTTGCAATGAGTACCGCGAAGTTTCGTCCTGTTCTTCTTGTGGTGACTTTCAAGCACGCAGAGCAATGATCCGCTATAAGGACTTAACCACAGGCAAGAATACCTATGTGGCTACCTTAAACGGCACCGCTTTAGCGATTGACCGCATCTTTGCAGCCATCTTGGAACACTACCAAACGAAGGCAGGGGAAGTAATGATTCCCCAAGCCTTGTTGAAATACTTAGACTTTGACAAAATTACCAAACCAAAATAA", "aa": "MPVIVNETSLTGTGQLPKFKDDVFKLENTRYYLSPTLEVQLANLHANEIFTEGELPKYYTATGVNFRQEAGSAGKQTKGTIRLHQFQKVELVKFCKPSEAIHELEEMTRDAEQILLELKIPFRRLLLCSGDMGFSAQKTYDLEVWMAGCNEYREVSSCSSCGDFQARRAMIRYKDLTTGKNTYVATLNGTALAIDRIFAAILEHYQTKAGEVMIPQALLKYLDFDKITKPK", "aa_hexdigest": "8ec45be77fe2508c618d0add3afe44e1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4FJT5", "query_cov": 0.9567099567099567, "subject_cov": 0.8804780876494024, "identity": 0.588, "score": 255.0, "evalue": 1.68e-82, "valid": false, "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_A0A540V7F7", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_A0A540V7F7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A540V7F7" ], "product": "Serine--tRNA ligase" }, "genes": [], "id": "GIHJHMOALN_50", "locus": "GCA009939745_00008" }, { "type": "cds", "sequence": "contig_1", "start": 8503, "stop": 8799, "strand": "+", "frame": 1, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_P47252", "UniRef:UniRef90_P75106" ], "nt": "ATGAAACAAGGCGTGTTTGTGGCCATAGAGGGCGTGGATGGTGCGGGGAAAACCGTCCTCCTAGAAGCTTTTAAACAGCGCTTTCCCCAAAGCTTTTTGGGCTTTAAAACGCTTTTTAGCAGGGAACCCGGGGGCACACCGTTAGCGGAAAAAATCCGGGCACTGCTCCTGCATGAAGCAATGGAACCACTTACAGAAGCTTATCTGTTTGCGGCATCGCGCACCGAACACGTGCGCCAGTTAATCCAACCAGCGTTACAACAAAAACAGTTAGTGATAGTAGACCGCTTTGTGTGA", "aa": "MKQGVFVAIEGVDGAGKTVLLEAFKQRFPQSFLGFKTLFSREPGGTPLAEKIRALLLHEAMEPLTEAYLFAASRTEHVRQLIQPALQQKQLVIVDRFV", "aa_hexdigest": "2021ebc7f345e281ef75aac8228ee1cf", "start_type": "ATG", "rbs_motif": null, "genes": [ "tmk" ], "truncated": "3-prime", "pseudogene": { "start": 8503, "stop": 9102, "observations": { "mutation": [ 8797 ], "stop": [ 8797 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75106", "query_cov": 1.0, "subject_cov": 0.4666666666666667, "identity": 1.0, "score": 191.0, "evalue": 3.93e-60, "gene_start": 1, "gene_end": 98, "reference_start": 1, "reference_end": 98, "reference_sequence": "MKQGVFVAIEGVDGAGKTVLLEAFKQRFPQSFLGFKTLFSREPGGTPLAEKIRALLLHEAMEPLTEAYLFAASRTEHVRQLIQPALQQKQLVIVDRFVWSSYAYQGLIKKVGLDVVKKLNADAVGDSMPDFTFIVDCDFETALNRMAKRGQDNLLDNTVKKQADFNTMRQYYHSLVDNKRVFLLDGQNQTGCLEQFIEQLSQCLTQPTLS" }, "paralog": false, "identity": 0.9949748743718593, "score": 390.0, "evalue": 4.06e-141, "description": "Internal stop codon at 8797. Nonsense mutation around 8797.", "psc": { "uniref90_id": "UniRef90_P75106", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_P47252", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75106", "EC:2.7.4.9", "UniRef:UniRef50_P47252", "COG:COG0125", "COG:F", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_P47252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47252" ], "product": "Thymidylate kinase" } }, "id": "GIHJHMOALN_51", "locus": "GCA009939745_00009" }, { "type": "cds", "sequence": "contig_1", "start": 8845, "stop": 9135, "strand": "+", "frame": 1, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_P47252", "UniRef:UniRef90_P75106" ], "nt": "GTGGTCAAAAAGCTCAATGCTGATGCGGTGGGGGATAGTATGCCCGACTTTACCTTTATTGTTGATTGTGACTTTGAGACTGCTTTAAACCGGATGGCCAAACGCGGTCAGGACAATTTATTGGACAATACTGTCAAAAAGCAAGCGGACTTTAATACTATGCGCCAGTATTACCACAGTTTGGTTGATAACAAACGCGTCTTTCTCTTAGACGGGCAAAACCAAACGGGTTGTCTGGAACAATTTATTGAACAATTAAGCCAATGTTTAACCCAACCCACGCTCTCTTAA", "aa": "MVKKLNADAVGDSMPDFTFIVDCDFETALNRMAKRGQDNLLDNTVKKQADFNTMRQYYHSLVDNKRVFLLDGQNQTGCLEQFIEQLSQCLTQPTLS", "aa_hexdigest": "267853f6b6a38c42afecbf2e79d4afa8", "start_type": "GTG", "rbs_motif": "AAAAA", "genes": [ "tmk" ], "truncated": "5-prime", "pseudogene": { "start": 8545, "stop": 9135, "observations": { "mutation": [ 8797 ], "stop": [ 8797 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75106", "query_cov": 1.0, "subject_cov": 0.45714285714285713, "identity": 0.99, "score": 193.0, "evalue": 4.43e-61, "gene_start": 1, "gene_end": 96, "reference_start": 115, "reference_end": 210, "reference_sequence": "MKQGVFVAIEGVDGAGKTVLLEAFKQRFPQSFLGFKTLFSREPGGTPLAEKIRALLLHEAMEPLTEAYLFAASRTEHVRQLIQPALQQKQLVIVDRFVWSSYAYQGLIKKVGLDVVKKLNADAVGDSMPDFTFIVDCDFETALNRMAKRGQDNLLDNTVKKQADFNTMRQYYHSLVDNKRVFLLDGQNQTGCLEQFIEQLSQCLTQPTLS" }, "paralog": false, "identity": 0.9948979591836735, "score": 385.0, "evalue": 5.09e-139, "description": "Internal stop codon at 8797. Nonsense mutation around 8797.", "psc": { "uniref90_id": "UniRef90_P75106", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_P47252", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75106", "EC:2.7.4.9", "UniRef:UniRef50_P47252", "COG:COG0125", "COG:F", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_P47252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47252" ], "product": "Thymidylate kinase" } }, "id": "GIHJHMOALN_52", "locus": "GCA009939745_00010" }, { "type": "cds", "sequence": "contig_1", "start": 9108, "stop": 9866, "strand": "+", "frame": 3, "gene": "holB", "product": "DNA polymerase III subunit delta'", "db_xrefs": [ "COG:COG0470", "COG:L", "EC:2.7.7.7", "SO:0001217", "UniRef:UniRef50_P47253", "UniRef:UniRef90_P75105" ], "nt": "ATGTTTAACCCAACCCACGCTCTCTTAATTATTCAAAGACGGGGTAGCTATCTCCAACCAGTTTTAACGGAGTACCTAACCCGGGTCGTTTGTGAACAGCAAACGGGGTGTCAAACATGTCCGAGTTGCTTGGAAATTCTCCATGGTACTTACAACAACTTTTACAGTTTTGACCAAGCCAACCCCTTTAAAAGGGAACATGCCTTGCACCTTAGTGAAGTGTTGAACCGCCAAAGTGAATCCAACCAAAAACAGCTGTACCTGATTAAAAACTTAGAAACGCTCACCGCTACGGCCATGAACAGCTTGCTCCGTTTAATTGAGGAACACCCGGTGAATACCTATGGTGTTTTTACCACGAAAAACGAAAACATGATCTTACCCACCATTTTGAGTCGCGTCCAAAAAGTAGTACTCAAAAAAGCGACCCAGTTACCGTTCCAAGTGGACAGTAAGGACCAGGCGATTCTCAAAAGTTTCTTTAGTGTAGATGAACAGTTACAAGCATTGGACAACGGTAGTTTTACCCGCTTAAAAACGATTATTACGACACTTACCAATAAGAAAAACACAGCGAGCACGGTTCACGAGGCTTGGGTGTTGTTTAAACAATTAAACCAAACGGAAACGGCTCAAGTACTGAACTTTATGGTGGATTACACCAAGGACTTAACCAAAAAGGACCGCTTGTTAAACATGGTGCAAAATTTGGTGTTTAACCCACCGAAAGCAGCGTTGTTTGCTAACCTCATTAACTGA", "aa": "MFNPTHALLIIQRRGSYLQPVLTEYLTRVVCEQQTGCQTCPSCLEILHGTYNNFYSFDQANPFKREHALHLSEVLNRQSESNQKQLYLIKNLETLTATAMNSLLRLIEEHPVNTYGVFTTKNENMILPTILSRVQKVVLKKATQLPFQVDSKDQAILKSFFSVDEQLQALDNGSFTRLKTIITTLTNKKNTASTVHEAWVLFKQLNQTETAQVLNFMVDYTKDLTKKDRLLNMVQNLVFNPPKAALFANLIN", "aa_hexdigest": "b3da2a21c3b77df301930721acf99695", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75105", "query_cov": 1.0, "subject_cov": 0.9960474308300395, "identity": 1.0, "score": 494.0, "evalue": 1.25e-176, "valid": true, "gene": "HolB", "product": "DNA polymerase III subunit delta'", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47253", "cog_id": "COG0470", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_P47253", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47253" ], "product": "Uncharacterized protein MG007" }, "genes": [ "holB" ], "id": "GIHJHMOALN_53", "locus": "GCA009939745_00011" }, { "type": "cds", "sequence": "contig_1", "start": 9871, "stop": 11199, "strand": "+", "frame": 1, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "COG:COG0486", "COG:J", "EC:3.6.-.-", "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872", "RefSeq:WP_014325281.1", "SO:0001217", "UniParc:UPI0001DD6442", "UniRef:UniRef100_A0AAV5N6V1", "UniRef:UniRef50_P47254", "UniRef:UniRef90_P75104" ], "nt": "ATGGACACCAAACAAACCATGTTTGCCCTCGCTACTGCGCCCTTCAACAGTGCCATTCACATTATTAGGTTATCTGGTCCCGATGTTTATCGCATTATTAACCAAATAACCAATAAGGAAGTCAAACCGTTGGGTATGCGGATTCAACGCGTTTGGTTAATTGATCACAACCAGAAAAAAGTGGATGATGTGTTGCTGTTTAAATTTGTCGCACCTAACTCTTACACTGGGGAAGATCTGATTGAAATTTCGTGCCATGGCAGCATGGTAATAGTCAACGAAATTATTGGTTTATTGTTAAAGCACGGGGCGGTACAAGCCCAACCCGGGGAGTTTACGCAGCGCGGCTATCTCAACGGTAAGATGAGTTTAAACCAAGCGGCTTCTGTTAATAACTTAGTACTTTCACCGAATACAACCCTAAAGGATGTCGCTTTAAATGCGTTAGCTGGTCAAGTTGATGCCAGGTTAGAACCCTTAGTGGAAAAGTTGGGTCAACTAGTAATGCAAATGGAAGTGAACTTGGATTACCCCGAGTACACTGATGAACAGCGCGAACTGGTGACAATGAACCAAGCAGTCTTGCAAATAACCCAAATCTTAAACCAAATCGTGGTTGGTCAAGACCAGTTACAACGGCTCAAAGATCCGTTTAAGATTGCCATTATTGGTAATACTAATGTTGGTAAGTCCTCCTTATTGAATGCTCTGTTAGATCAAGATAAAGCGATAGTATCCGCCATTAAAGGCAGTACCCGTGACATTGTTGAGGGTGACTTTGCTCTAAACGGTCACTTCGTCAAAATCCTCGACACAGCGGGAATTCGCCAACACCAAAGTGCGCTCGAAAAAGCGGGCATTCAAAAAACCTTTGGCGCCATTAAAACCGCTAACTTAGTAATTTACCTTTTGGATGCCCGGCAGCCAGAACCTGATCCCAAGATTATTGCGCGATTAAAGAAGCTGAAGAAGGACTTCTTTTTGGTCCACAATAAAGCAGACTTAGTGCAGCAAAGCTTTCAGGTAAGTATTTCTGCCAAACAAAAACAGATCCAACCACTTGTCGATTTATTAACCCAATACCTACACCAGTTTTACAGTGTGGAGCAGAACCAGCTCTACTTAATTAGTGATTGGCAAACGATCCTGCTCCAAAAAGCAATAGCCGAACTAGAACATTTTTTAATTAAACAACAAAACTGCCTTTTCTTTGATATCCTAGTTGTTCATTTACGTGCTGCTCACGAATACATTCTCCAAGTCTTGGGTAAAAACACCAACTATGACCTGATTAACGAAATCTTTAAGCACTTTTGCTTAGGTAAGTAA", "aa": "MDTKQTMFALATAPFNSAIHIIRLSGPDVYRIINQITNKEVKPLGMRIQRVWLIDHNQKKVDDVLLFKFVAPNSYTGEDLIEISCHGSMVIVNEIIGLLLKHGAVQAQPGEFTQRGYLNGKMSLNQAASVNNLVLSPNTTLKDVALNALAGQVDARLEPLVEKLGQLVMQMEVNLDYPEYTDEQRELVTMNQAVLQITQILNQIVVGQDQLQRLKDPFKIAIIGNTNVGKSSLLNALLDQDKAIVSAIKGSTRDIVEGDFALNGHFVKILDTAGIRQHQSALEKAGIQKTFGAIKTANLVIYLLDARQPEPDPKIIARLKKLKKDFFLVHNKADLVQQSFQVSISAKQKQIQPLVDLLTQYLHQFYSVEQNQLYLISDWQTILLQKAIAELEHFLIKQQNCLFFDILVVHLRAAHEYILQVLGKNTNYDLINEIFKHFCLGK", "aa_hexdigest": "a72c9d6a2c9cf09ed7ea4eb120148f99", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6442", "ncbi_nrp_id": "WP_014325281.1", "uniref100_id": "UniRef100_A0AAV5N6V1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6442", "RefSeq:WP_014325281.1", "UniRef:UniRef100_A0AAV5N6V1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N6V1", "uniref90_id": "UniRef90_P75104" }, "psc": { "uniref90_id": "UniRef90_P75104", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "ec_ids": [ "3.6.-.-" ], "uniref50_id": "UniRef50_P47254", "cog_id": "COG0486", "cog_category": "J", "go_ids": [ "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47254" ], "product": "tRNA modification GTPase MnmE" }, "genes": [ "mnmE" ], "id": "GIHJHMOALN_54", "locus": "GCA009939745_00012" }, { "type": "cds", "sequence": "contig_1", "start": 11199, "stop": 11876, "strand": "+", "frame": 3, "gene": "tatD", "product": "putative metal-dependent hydrolase MPN_009", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.-.-", "GO:0004536", "GO:0005829", "GO:0046872", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_P47255", "UniRef:UniRef90_P78005" ], "nt": "ATGGAATACTTTGATGCGCACTGTCACTTAAACTGTGAACCCCTACTGGAACAGCACGAAAAGAGTTTGGCCAACTTTAGGTTAATTGGGTTAAAGGCCAATGTTGTGGGCACTAATTTAACTAACTCCCAAATTGCGGTGAATTTAGCTAAACAGCACCCTGATTTACTCAAGGCAGGGGTGGGTATTCACCCTAATGATGTCCAGCTTTTTGATTTAAAAGCAGCGCAAGCAACACTAGAAAAATTAGTAAGTACGCATCGCTCATTCATTAGTTGTATTGGTGAATATGGCTTTGATTACCACTACACTAAGGACTACATTACCCAACAAGAACAGTTCTTTTTGATGCAGTTCCAACTAGCAGAACAGTACCAGTTAGTGCACATGTTGCATGTCCGTGATGTGCACGAAAGGATATACGAAGTTTTGAAAAGGTTAAAACCTAAACAACCAGTGGTGTTTCACTGTTTTAGTGAAGACACCAATACTGCTTTAAAGCTGCTAACTTTAAGAGAAGTAGGACTAAAGGTTTATTTTTCGATTCCGGGGATTGTGACCTTTAAGAATGCGAAAAACTTGCAAGCCGCTTTATCAGTTATTCCAACAGAGCTACTTTTAAGTGAAACCGATTCGCCTTATTTAGCACCAGTACCTTTTCGGGGCAAAACTAATTGA", "aa": "MEYFDAHCHLNCEPLLEQHEKSLANFRLIGLKANVVGTNLTNSQIAVNLAKQHPDLLKAGVGIHPNDVQLFDLKAAQATLEKLVSTHRSFISCIGEYGFDYHYTKDYITQQEQFFLMQFQLAEQYQLVHMLHVRDVHERIYEVLKRLKPKQPVVFHCFSEDTNTALKLLTLREVGLKVYFSIPGIVTFKNAKNLQAALSVIPTELLLSETDSPYLAPVPFRGKTN", "aa_hexdigest": "446ae871dd19e49f0a599f40c2e6e3ca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P78005", "query_cov": 1.0044444444444445, "subject_cov": 0.8625954198473282, "identity": 0.991, "score": 443.0, "evalue": 1.09e-156, "valid": true, "gene": "TatD", "product": "Uncharacterized metal-dependent hydrolase MPN_009", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47255", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424", "go_ids": [ "GO:0005829", "GO:0004536", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47255" ], "product": "Uncharacterized metal-dependent hydrolase MG009" }, "genes": [ "tatD" ], "id": "GIHJHMOALN_55", "locus": "GCA009939745_00013" }, { "type": "cds", "sequence": "contig_1", "start": 12181, "stop": 12291, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTACTCGCCTTCGCTTAACGACATTAAGAGCATTTTGAACAAATATACCTCTAAGGACTATGAGTTAAAATGCGAAAATAGGTATGATGGCAAGCTTGAATTATGA", "aa": "MAYSPSLNDIKSILNKYTSKDYELKCENRYDGKLEL", "aa_hexdigest": "ef6ad5242060c2922cad14c787ae9fd9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4243.7669000000005, "isoelectric_point": 6.057411003112793 }, "id": "GIHJHMOALN_56", "locus": "GCA009939745_00014" }, { "type": "cds", "sequence": "contig_1", "start": 12316, "stop": 12576, "strand": "+", "frame": 1, "gene": null, "product": "DUF16 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6444", "UniRef:UniRef100_A0A0H3DL80", "UniRef:UniRef50_A0A0H3DL80", "UniRef:UniRef90_A0A0H3DL80" ], "nt": "GTGAAAACACCTGGCACTAGGTACGTCACCCATAAGCAACTTGATGAAAAGCTCAAAAATTTTGTCACTAAAACAGAATTTAAAGAGTTCCAAACTGTAGTTATGGAGAGTTTTGCTGTTCAAAACCAGAATATTGATGCTCAGGGTGAGCAGATCAAAGAACTTCAAGTTGAACAAAAAGCTCAAGGAAAGACCCTGCAATTGATACTTGAAGCTCTCCAGGGTATAAACAAACGTCTAGACAACTTAGAGTCCAAATAA", "aa": "MKTPGTRYVTHKQLDEKLKNFVTKTEFKEFQTVVMESFAVQNQNIDAQGEQIKELQVEQKAQGKTLQLILEALQGINKRLDNLESK", "aa_hexdigest": "75de747e0fb9f4f2ad11881c86013141", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6444", "uniref100_id": "UniRef100_A0A0H3DL80", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6444", "UniRef:UniRef100_A0A0H3DL80" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DL80", "uniref90_id": "UniRef90_A0A0H3DL80" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DL80", "product": "DUF16 domain-containing protein", "uniref50_id": "UniRef50_A0A0H3DL80" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DL80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DL80" ], "product": "DUF16 domain-containing protein" }, "genes": [], "id": "GIHJHMOALN_57", "locus": "GCA009939745_00015" }, { "type": "cds", "sequence": "contig_1", "start": 12884, "stop": 13048, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTATTCTTACAGAGGATTCAATATCCACAAAGGTGACAAACATTATTTCAACAGTTTCCTTACTCTTAAAGCGAATTCATATTTACCATTTACGTCAAAAAATTTTGATGTCTATTCCAAAAGAATTAGGTTATCTGTTTCGTTTCATTGAATACTAA", "aa": "MKIILTEDSISTKVTNIISTVSLLLKRIHIYHLRQKILMSIPKELGYLFRFIEY", "aa_hexdigest": "258acaabdd536dd76234507b2e94fe36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6421.700299999997, "isoelectric_point": 9.774519157409667 }, "id": "GIHJHMOALN_58", "locus": "GCA009939745_00016" }, { "type": "cds", "sequence": "contig_1", "start": 13224, "stop": 13457, "strand": "-", "frame": 2, "gene": null, "product": "lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019135978", "UniRef:UniRef90_UPI0019135978" ], "nt": "ATGAAATTTAAATTCTTATTAACCCCACTATTATCTTCAGTTTTATTTCTTAGTGCTTGTAGCGCCACTTTCGAAGCTGATCTCAAAAATCTTATTAAGGAAACAGATGGTAAAGACCTTGATGTTTCTAAATTAATTATTACTTCGGAAGGGAAACAAATCTTAATAGGTTATTTAAAAAAATCTTACGAAGTTAACTCTGAAAAAACTACTGAACTATTACTAAATGCCTGA", "aa": "MKFKFLLTPLLSSVLFLSACSATFEADLKNLIKETDGKDLDVSKLIITSEGKQILIGYLKKSYEVNSEKTTELLLNA", "aa_hexdigest": "e623a3d940a2248e5c58d780707e6d4c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 13164, "stop": 13442, "observations": { "mutation": [ 13178, 13226 ], "stop": [ 13178, 13226 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019135978", "query_cov": 0.935064935064935, "subject_cov": 0.7741935483870968, "identity": 1.0, "score": 135.0, "evalue": 5.17e-40, "gene_start": 6, "gene_end": 77, "reference_start": 2, "reference_end": 73, "reference_sequence": "MLLTPLLSSVLFLSACSATFEADLKNLIKETDGKDLDVSKLIITSEGKQILIGYLKKSYEVNSEKTTELLLNAWKQSQEKNQIGIDLFNWTKS" }, "paralog": false, "identity": 0.9565217391304348, "score": 166.0, "evalue": 1.24e-54, "description": "Internal stop codon at 13178, 13226. Nonsense mutation around 13178, 13226.", "psc": { "uniref90_id": "UniRef90_UPI0019135978", "product": "lipoprotein", "uniref50_id": "UniRef50_UPI0019135978", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019135978", "UniRef:UniRef50_UPI0019135978" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0019135978", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019135978" ], "product": "lipoprotein" } }, "id": "GIHJHMOALN_59", "locus": "GCA009939745_00017" }, { "type": "cds", "sequence": "contig_1", "start": 13629, "stop": 13841, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATCAAGATCTTAATAATTTTTCGATAAATGCTAAGTTGAACTATAACTTTAATTGGCATGGAAATTATTCTTCCAATGGGTTTGCTGCCAAAAAAGGTGACAAACATGTTTTTGATCTTCCTCTTGCAATTAAGCCATCTTCAAAGAACCAATCTAAAACGATAAGTTTCATTACTAAGACAGGGGAAGGCGTTGACACAGAATGA", "aa": "MEYQDLNNFSINAKLNYNFNWHGNYSSNGFAAKKGDKHVFDLPLAIKPSSKNQSKTISFITKTGEGVDTE", "aa_hexdigest": "a387d55e91d6b487754fcba3a42cbbf1", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7872.618200000002, "isoelectric_point": 8.00570011138916 }, "id": "GIHJHMOALN_60", "locus": "GCA009939745_00018" }, { "type": "cds", "sequence": "contig_1", "start": 13959, "stop": 14189, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCEF156", "PFAM:PF00938.23" ], "nt": "ATGAAATTTAAATACCTTTCAATTCCGTTTTTATCTTCAACTTTATTGTTTAGTGCTTATGCTTCTATCCAGTCTGATCTAAGAAATCTTATTCAAGAAACCACTAAACAAGACGTTGATGTGTACAAAGTAATCAAAACTTCTGAAGGCAGAAAAAACTTAATAACTTCTCTAAAGAAATCTTACGAAGTTAATCCTGATAAAACCACAAAACTATTGTTAGATGCCTGA", "aa": "MKFKYLSIPFLSSTLLFSAYASIQSDLRNLIQETTKQDVDVYKVIKTSEGRKNLITSLKKSYEVNPDKTTKLLLDA", "aa_hexdigest": "7003eb05d30df8ae726557f53f8a5aab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCEF156", "query_cov": 0.9473684210526315, "subject_cov": 0.9863013698630136, "identity": 0.708, "score": 94.7, "evalue": 4.09e-24, "valid": false, "uniref50_id": "UniRef50_UPI001FCEF156" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCEF156", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCEF156" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 32, "aa_cov": 0.42105263157894735, "hmm_cov": 1.0, "evalue": 1.3664052663174838e-06, "score": 23.492643356323242, "start": 45, "stop": 76 } ], "seq_stats": { "molecular_weight": 8689.978299999995, "isoelectric_point": 9.435415458679199 }, "id": "GIHJHMOALN_61", "locus": "GCA009939745_00019" }, { "type": "cds", "sequence": "contig_1", "start": 14701, "stop": 14916, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F9400A", "UniRef:UniRef100_A0AB38W8J2", "UniRef:UniRef50_A0AB38W8J2", "UniRef:UniRef90_A0AB38W8J2" ], "nt": "ATGTTGTTTTGTTTGAAGTCTCTTTACTTTAAATTGCAAAAATTTTTAGCAATTTTATTGGCAGTTTTTGAAATTCTGGTCAATTTATTTAGTGAAGGAAAAATTATGTCTTTACTCAATATATTTATTAGATCAACGATGATTTTGATCCTTGCTGGCAACAAAGTTGCACGTTTTAGTTTTCTAAACCTTGAGCAGAAAGGAGGAATTTATTAA", "aa": "MLFCLKSLYFKLQKFLAILLAVFEILVNLFSEGKIMSLLNIFIRSTMILILAGNKVARFSFLNLEQKGGIY", "aa_hexdigest": "05c35bec620a92818791b77a240368a2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002F9400A", "uniref100_id": "UniRef100_A0AB38W8J2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F9400A", "UniRef:UniRef100_A0AB38W8J2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8J2", "uniref90_id": "UniRef90_A0AB38W8J2" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8J2", "uniref50_id": "UniRef50_A0AB38W8J2" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8J2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8J2" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 14623, "stop": 14916, "observations": { "mutation": [ 14683 ], "stop": [ 14683 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8J2", "query_cov": 1.0, "subject_cov": 0.7319587628865979, "identity": 0.986, "score": 126.0, "evalue": 1.46e-36, "gene_start": 1, "gene_end": 71, "reference_start": 27, "reference_end": 97, "reference_sequence": "HLRDVNTSLMFLNTLKNLFGWLGSLLMLFCLKSLYFKLQKFLAILLAVFEILVNLFSEGKIMSLLNIFIRSTMMLILAGNKVARFSFLNLEQKGGIY" }, "paralog": false, "identity": 0.979381443298969, "score": 182.0, "evalue": 5.22e-61, "description": "Internal stop codon at 14683. Nonsense mutation around 14683.", "psc": { "uniref90_id": "UniRef90_A0AB38W8J2", "uniref50_id": "UniRef50_A0AB38W8J2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8J2", "UniRef:UniRef50_A0AB38W8J2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8J2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8J2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8148.923999999995, "isoelectric_point": 9.806624412536618 }, "id": "GIHJHMOALN_62", "locus": "GCA009939745_00020" }, { "type": "cds", "sequence": "contig_1", "start": 14916, "stop": 15689, "strand": "+", "frame": 3, "gene": null, "product": "UPF0134 protein MPN_013", "db_xrefs": [ "RefSeq:WP_014325283.1", "SO:0001217", "UniParc:UPI0001DD6445", "UniRef:UniRef100_A0AAV5N6T9", "UniRef:UniRef50_P75100", "UniRef:UniRef90_P75100" ], "nt": "ATGCCTAAAGTTATTTCCCAAAAGGAATTTAATATCCTTTCCCGGACAAAGGAAAGAGTTAATTTTCAAATTGTCTATACCAAGCAAAGAAATGATTTGCACAAAGCTGCTAAGGAGTACTTTTTTAAAGACAAAGACTTTACAATAATAGAAGATACACCGGACACACCTGACCAACCAGATGGACCATGTATTACTAAAGGCCCAAAGGAACCAGGTGGTCCTGAAGAGCCAAATGGTCCGGATAAACCAGAAGATCCTAAAGATCCTGATACGCCAGATGTTTCTGAAGGCCCTAAAGAACAAGGTGGTCAGAAAGATCCAGGTGGTCCGGATGATCCCAATGAAGGAAAGCAGAAAGTGTCCAAACCAGATAAATACGTAACCCACCGTGAGCTTGATGAAAAGCTTAAAGACTTTGCCACCAAAGCTGATTTCAAACGCGTCGAAGACAAAGTTGATGTTTTATTTGAACTGCAAAAAACGCAAGGCGAACAAATAAAGGTTCAAGGTGAGCAGATCAAAGCTCAAGGAAAACAAATTGAACAATTAACTGAAACTGTCAAAGTGCAAGGCGAGCAGATTAGGGCTCAAGGAGAACAAATCAAAGCTCAGAGCGAAGAAATAAAAGAAATTAAGGTTGAGCAAAAAGCCCAAGGCGAGCAAATCAAAGAACTTCAAGTAGAACAAAAAGCCCAAGGTAAAACCCTACAGTTAATTTTAAAGACGCTAGAAAAAATGAACGAGCGTCTAGAAAAATTGGAATCTAAATAG", "aa": "MPKVISQKEFNILSRTKERVNFQIVYTKQRNDLHKAAKEYFFKDKDFTIIEDTPDTPDQPDGPCITKGPKEPGGPEEPNGPDKPEDPKDPDTPDVSEGPKEQGGQKDPGGPDDPNEGKQKVSKPDKYVTHRELDEKLKDFATKADFKRVEDKVDVLFELQKTQGEQIKVQGEQIKAQGKQIEQLTETVKVQGEQIRAQGEQIKAQSEEIKEIKVEQKAQGEQIKELQVEQKAQGKTLQLILKTLEKMNERLEKLESK", "aa_hexdigest": "ff5daef767ae27e0b646c6ca7ec8506d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6445", "ncbi_nrp_id": "WP_014325283.1", "uniref100_id": "UniRef100_A0AAV5N6T9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6445", "RefSeq:WP_014325283.1", "UniRef:UniRef100_A0AAV5N6T9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N6T9", "uniref90_id": "UniRef90_P75100" }, "psc": { "uniref90_id": "UniRef90_P75100", "product": "UPF0134 protein MPN_013", "uniref50_id": "UniRef50_P75100" }, "pscc": { "uniref50_id": "UniRef50_P75100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75100" ], "product": "UPF0134 protein MPN_013" }, "genes": [], "id": "GIHJHMOALN_63", "locus": "GCA009939745_00021" }, { "type": "cds", "sequence": "contig_1", "start": 15791, "stop": 16363, "strand": "+", "frame": 2, "gene": "dnaG", "product": "putative MG010-like protein", "db_xrefs": [ "COG:COG0358", "COG:L", "GO:0003896", "GO:0005737", "SO:0001217", "UniRef:UniRef50_P75099", "UniRef:UniRef90_P75099" ], "nt": "ATGGAACTTAGAAATACAAAATTAGAACAACTCTTTTACATCTATCACCGCAACCTGAAGTATAGATGTCCAATGCAGTACCTCACTAAAAGGGGCTTTAACTTACAAGACCTCTTGTCAGTTGGTGGTGGATTAGCTTACTTGGGCGAAAAACAGTGGTTAAATTTATCACTGTACAACTTTAACAACCAGTTAGTTGGTTTTTTAAGTCGGAAGGTAGGTTTTGAAAAGAAGTTCCTTTATTTACCTATTAATAAACCACCAAGTAAAAGTGAATCTTTTTTAGGTTTAAAACACTTACCAACGGAAACGAACACCATTTACCTAGTTGAAGGTGATTTTGATTGGTTAGCTTTCCGTAAGGGTGGTATTTTGAACTGCTTGCCTTTATGTGGATTAACTTTATCAGATAAACAAATGAAGTTTTTTCAACAAAGTAAGATCGAAAAAGTGGTGCTTTGCTTAGACAATGATTTTGCTGGTAAAGTAGCTGCTGCTAACCTGGAACGCATTTTAAAAAATGCTGGTTTTCAGGTTAAGGTAGTTCAGCTAAAGGGTAAAGTTAAGGACTGA", "aa": "MELRNTKLEQLFYIYHRNLKYRCPMQYLTKRGFNLQDLLSVGGGLAYLGEKQWLNLSLYNFNNQLVGFLSRKVGFEKKFLYLPINKPPSKSESFLGLKHLPTETNTIYLVEGDFDWLAFRKGGILNCLPLCGLTLSDKQMKFFQQSKIEKVVLCLDNDFAGKVAAANLERILKNAGFQVKVVQLKGKVKD", "aa_hexdigest": "88f2bd83a79eaee97ec33021e7f80ae2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75099", "query_cov": 1.0, "subject_cov": 0.8962264150943396, "identity": 1.0, "score": 389.0, "evalue": 9.48e-137, "valid": true, "gene": "DnaG", "product": "Uncharacterized protein MG010 homolog", "uniref50_id": "UniRef50_P75099", "cog_id": "COG0358", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0003896" ] }, "pscc": { "uniref50_id": "UniRef50_P75099", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75099" ], "product": "Uncharacterized protein MG010 homolog" }, "genes": [ "dnaG" ], "id": "GIHJHMOALN_64", "locus": "GCA009939745_00022" }, { "type": "cds", "sequence": "contig_1", "start": 16406, "stop": 16630, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCACAAAATTAAACGGATTGCTAAAAAGCTAGGTCATGGTTACTACGCCATTGACTTCTTCTTAAATAAGTACAACCGAATAGTGGTCAATGAAATCGAGGATGCTGCTGGTGCTAGGGCACTAACCAAAATGTGTCCTGACTTAAACCTACCAAGGGTAATTATTAAATCGTCCTTAACTCACTTTAAACATCATTTAAAGCGCCAAATGATCCCTTAA", "aa": "MKHKIKRIAKKLGHGYYAIDFFLNKYNRIVVNEIEDAAGARALTKMCPDLNLPRVIIKSSLTHFKHHLKRQMIP", "aa_hexdigest": "7e6ec999ceeedb8b58d445c113b12acb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8642.251299999996, "isoelectric_point": 10.201557731628416 }, "id": "GIHJHMOALN_65", "locus": "GCA009939745_00023" }, { "type": "cds", "sequence": "contig_1", "start": 16634, "stop": 17263, "strand": "-", "frame": 3, "gene": "lysX", "product": "putative MG011-like protein", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47257", "UniRef:UniRef90_P75098" ], "nt": "ATGGCCAAAATTAAGTTAAAAAACAGAAAAGCACTCGTTGTTTACAATAAGACCGATTTTGACAAGAACAAGCACTTTGCTCAAGCACTAGTCGATGAACTGAACAAGAAAAAACTAGTTGGGCATATTTTGCTGTTAGATGATGAAACCGCTGATCACAAACACATTAAAAATGTGGAATTGATCATTAACCGTTCGCGCAGAATTGATTTTTTAACCAAACACAACTTTCTCAACAGTTTTCTCATTAATCCCCAAAATATCGTTTTGGTAGCCAATGATAAGTATGAAACTTACAGGTGGTTAAAGCAGCATAAGTTCTTGACCGTTGATACCACCATTTTTGATCCCAAAAAGATTAAGACTTTTCCGATTGTGATCAAAAAGCGTGATTCTCATGGTGGGGAAGATGTGCACTTAATTCAAAATGCGGAAGAAATTAAGCAGTTACCAATTCAAAATCCTAATGAGTGGATTGTCCAACCATTTTTGTCAATTGGTAAAGTGGAATACCGGGCTTACATCTTGTTTGGTAAGGTGTTAAAAACAATTAGAAGGACTGCTAGTGGTGATGATTTTAGGGCTAACTACTCACAAAATGCTGCTGTTGATTTGTTTAAACTAAAGTGA", "aa": "MAKIKLKNRKALVVYNKTDFDKNKHFAQALVDELNKKKLVGHILLLDDETADHKHIKNVELIINRSRRIDFLTKHNFLNSFLINPQNIVLVANDKYETYRWLKQHKFLTVDTTIFDPKKIKTFPIVIKKRDSHGGEDVHLIQNAEEIKQLPIQNPNEWIVQPFLSIGKVEYRAYILFGKVLKTIRRTASGDDFRANYSQNAAVDLFKLK", "aa_hexdigest": "e0883f97cc6f6a5e67c92068a48361f8", "start_type": "ATG", "rbs_motif": null, "genes": [ "lysX" ], "truncated": "5-prime", "pseudogene": { "start": 16406, "stop": 17263, "observations": { "mutation": [ 16636 ], "stop": [ 16636 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75098", "query_cov": 1.0, "subject_cov": 0.7333333333333333, "identity": 0.995, "score": 417.0, "evalue": 1.47e-146, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MAKIKLKNRKALVVYNKTDFDKNKHFAQALVDELNKKKLVGHILLLDDETADHKHIKNVELIINRSRRIDFLTKHNFLNSFLINPQNIVLVANDKYETYRWLKQHKFLTVDTTIFDPKKIKTFPIVIKKRDSYGGEDVHLIQNAEEIKQLPIQNPNEWIVQPFLSIGKVEYRAYILFGKVLKTIRRTASGDDFRANYSQNAAVDLFKLKWYIKHKIKRIAKKLGHGYYAIDFFLNKYNRIVVNEIEDAAGARALTKMCPDLNLPRVIIKSSLTHFKHHLKRQMIP" }, "paralog": false, "identity": 0.9894736842105263, "score": 567.0, "evalue": 5.82e-208, "description": "Internal stop codon at 16636. Nonsense mutation around 16636.", "psc": { "uniref90_id": "UniRef90_P75098", "gene": "LysX", "product": "Uncharacterized protein MG011 homolog", "uniref50_id": "UniRef50_P47257", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75098", "UniRef:UniRef50_P47257", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P47257", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47257" ], "product": "Uncharacterized protein MG011" } }, "id": "GIHJHMOALN_66", "locus": "GCA009939745_00024" }, { "type": "cds", "sequence": "contig_1", "start": 17263, "stop": 17751, "strand": "-", "frame": 1, "gene": "lysX", "product": "putative MG012-like protein", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0036211", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75097", "UniRef:UniRef90_P75097" ], "nt": "TTGGAACAATTCATCGAACAAATTAAGCGCTGTTTTCAGTTTCCAGTGATTGTCAAAAGTATCTATGGTTCTTTTGGTGATTACGTTTTTTTGTGCAAAGATGAACAGAAGTTACGTCAAACCTTAAGCGGGCTAACTGAACAGATCATTGTGCAGCAATACATTGCCACTTCTAATAGTGAAGCGGTCAGGGTAATTGTGGTCAATAACCAGGTAGTTGGGGCCTTACATACTCAAAATGAGGGTGATTTTCGCTCTAATTTAAATAAGGGAGCGGTTGGTGAACCATATCAACTAAGTCAGGAAGAAACCAAATTAGCAATTACAATTAGCCAAGCAATGCAACTCTTTTATTGTGGCATTGACTTTTTATTTGACCAGGATCGTTCGTTAATTTTCTGCGAAGTTAATTCCAATGTACAGCTCACCAAAAGCTCGATGTATTTGAAAACTAACCTCGCCATTCAACTTTTAGCATCAATCGCATAA", "aa": "MEQFIEQIKRCFQFPVIVKSIYGSFGDYVFLCKDEQKLRQTLSGLTEQIIVQQYIATSNSEAVRVIVVNNQVVGALHTQNEGDFRSNLNKGAVGEPYQLSQEETKLAITISQAMQLFYCGIDFLFDQDRSLIFCEVNSNVQLTKSSMYLKTNLAIQLLASIA", "aa_hexdigest": "da3b53ab4ef40bccb9279677950a0a35", "start_type": "TTG", "rbs_motif": null, "genes": [ "lysX" ], "truncated": "3-prime", "pseudogene": { "start": 17263, "stop": 18051, "observations": { "mutation": [ 17766 ], "stop": [ 17766 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75097", "query_cov": 1.0, "subject_cov": 0.5625, "identity": 0.9940000000000001, "score": 313.0, "evalue": 3.07e-106, "gene_start": 1, "gene_end": 162, "reference_start": 127, "reference_end": 288, "reference_sequence": "MKHISAVYNPAFTNIASKLNQTELLKDAAQSLNIQLDFFTCFDINTNQDKTKLPFKSNTILFLDKNIALAQWLESVGLRVINSSIAINNADNKALSHAVLAQHPTIKQIPTLIGPQNFRLAWYPEKLEQFIEQIKRCFQFPVIVKSIYGSFGDYVFLCKDEQKLRQTLSGLTEQIIVQQYIATSNSEAVRVIVVNNQVVGALHTQNEGDFRSNLNKGAVGEPYQLSQEETKLAITISQAMQLFYCGIDFLFDQDRSLIFCEVNSNVQLTKSSMYLKTNLAIQLLASIA" }, "paralog": false, "identity": 0.9961832061068703, "score": 510.0, "evalue": 3.62e-186, "description": "Internal stop codon at 17766. Nonsense mutation around 17766.", "psc": { "uniref90_id": "UniRef90_P75097", "gene": "LysX", "product": "Uncharacterized protein MG012 homolog", "uniref50_id": "UniRef50_P75097", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75097", "UniRef:UniRef50_P75097", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75097", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75097" ], "product": "Uncharacterized protein MG012 homolog" } }, "id": "GIHJHMOALN_67", "locus": "GCA009939745_00025" }, { "type": "cds", "sequence": "contig_1", "start": 17764, "stop": 18129, "strand": "-", "frame": 1, "gene": "lysX", "product": "putative MG012-like protein", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0036211", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75097", "UniRef:UniRef90_P75097" ], "nt": "ATGAAGCACATCAGCGCCGTATATAACCCTGCTTTTACTAACATTGCTTCCAAATTAAACCAAACGGAACTGTTAAAGGATGCAGCCCAGAGCTTAAACATCCAACTCGATTTCTTTACTTGCTTTGATATTAATACCAATCAGGATAAGACCAAACTACCCTTTAAGTCCAATACCATTTTGTTTTTGGATAAAAACATAGCGTTAGCGCAATGGTTAGAATCAGTTGGTTTGCGCGTTATTAACAGTTCGATTGCCATTAATAACGCGGACAATAAGGCCTTGAGTCATGCTGTTTTAGCGCAACACCCAACCATTAAACAGATTCCTACATTAATTGGCCCACAGAACTTTCGTTTAGCTTGA", "aa": "MKHISAVYNPAFTNIASKLNQTELLKDAAQSLNIQLDFFTCFDINTNQDKTKLPFKSNTILFLDKNIALAQWLESVGLRVINSSIAINNADNKALSHAVLAQHPTIKQIPTLIGPQNFRLA", "aa_hexdigest": "e3eadba98d3468ed554dbc7a60e2d616", "start_type": "ATG", "rbs_motif": "AAAAA", "genes": [ "lysX" ], "truncated": "5-prime", "pseudogene": { "start": 17461, "stop": 18129, "observations": { "mutation": [ 17766 ], "stop": [ 17766 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75097", "query_cov": 1.0, "subject_cov": 0.4201388888888889, "identity": 1.0, "score": 241.0, "evalue": 3e-78, "gene_start": 1, "gene_end": 121, "reference_start": 1, "reference_end": 121, "reference_sequence": "MKHISAVYNPAFTNIASKLNQTELLKDAAQSLNIQLDFFTCFDINTNQDKTKLPFKSNTILFLDKNIALAQWLESVGLRVINSSIAINNADNKALSHAVLAQHPTIKQIPTLIGPQNFRLAWYPEKLEQFIEQIKRCFQFPVIVKSIYGSFGDYVFLCKDEQKLRQTLSGLTEQIIVQQYIATSNSEAVRVIVVNNQVVGALHTQNEGDFRSNLNKGAVGEPYQLSQEETKLAITISQAMQLFYCGIDFLFDQDRSLIFCEVNSNVQLTKSSMYLKTNLAIQLLASIA" }, "paralog": false, "identity": 0.9954954954954955, "score": 435.0, "evalue": 3.56e-157, "description": "Internal stop codon at 17766. Nonsense mutation around 17766.", "psc": { "uniref90_id": "UniRef90_P75097", "gene": "LysX", "product": "Uncharacterized protein MG012 homolog", "uniref50_id": "UniRef50_P75097", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75097", "UniRef:UniRef50_P75097", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75097", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75097" ], "product": "Uncharacterized protein MG012 homolog" } }, "id": "GIHJHMOALN_68", "locus": "GCA009939745_00026" }, { "type": "cds", "sequence": "contig_1", "start": 18197, "stop": 18811, "strand": "-", "frame": 3, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "COG:COG0190", "COG:H", "GO:0000105", "GO:0004477", "GO:0004488", "GO:0005829", "GO:0006164", "GO:0009086", "GO:0035999", "SO:0001217", "UniRef:UniRef50_P47259", "UniRef:UniRef90_P75096" ], "nt": "GTGTCATCTGATGCCTTTATTCGCCAAAAAATGAATGCTTGTAATACTCTAGGGGCCCAGAGTGAACTAATTAAGTACCACGAAAGTGTCACTGAGAAAGAATTGTTAGAAAAAATTCAACAACTCAACACTGATCCTGAGGTAACAGGGATTATCTTGCAACTGCCAGTGTATCCCCATTTAAAACAAAACAAGCTCTTAAAAGCAATTGATCCTTACAAGGATGTGGATTACTTAACTGGTAACTGCCCCCTACCCATTAGGTCCTGTGTGGTGGAAGCGGTGTTAATTTTGAAAGAACACTTTCACCATGATTTCACCAACAAGCAGATTGTGGTGGTGGGTTTAGGCGTCACTGGTGGCGGGCCGATCTTTCACTATTTAAAGGAAACGGGTCATCAGGTGGTGGGATGTGACAAGCATACCCCCAACACCATGGAGCTAATTAAAACCGCTGACGTGGTGATCACAGCCATTGGCAAGCCCCATTTCTTTAAGACCACTAACTTTAAACCAGGTGTTATTCTTTATGATGTGGGGGTATCACGTAATGCCTTAAACAAACTGTGTGGTGACATCGATCCTAATGGGATAGAGAAAGTTGCCAAATGGTGA", "aa": "MSSDAFIRQKMNACNTLGAQSELIKYHESVTEKELLEKIQQLNTDPEVTGIILQLPVYPHLKQNKLLKAIDPYKDVDYLTGNCPLPIRSCVVEAVLILKEHFHHDFTNKQIVVVGLGVTGGGPIFHYLKETGHQVVGCDKHTPNTMELIKTADVVITAIGKPHFFKTTNFKPGVILYDVGVSRNALNKLCGDIDPNGIEKVAKW", "aa_hexdigest": "e7056c48dbf7c84d900d48e48921404d", "start_type": "GTG", "rbs_motif": null, "genes": [ "folD" ], "truncated": "both", "pseudogene": { "start": 18104, "stop": 18913, "observations": { "mutation": [ 18139, 18199, 18853 ], "stop": [ 18139, 18199, 18853 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75096", "query_cov": 1.0, "subject_cov": 0.758364312267658, "identity": 0.99, "score": 416.0, "evalue": 2.76e-146, "gene_start": 1, "gene_end": 204, "reference_start": 35, "reference_end": 238, "reference_sequence": "MSFDGKQLAHEILLTYKNRDWSQVKLVILQTNNDVSSDAFIRQKMNACNTLGAQSELIKYHESVTEKELLEKIQQLNTDPEVTGIILQLPVYPHLKQNKLLKAIDPYKDVDYLTGNCPLPIRSCVVEAVLILKEHFHHDFTNKQIVVVGLGVTGGGPIFHYLKETGHQVVGCDKHTPNTMELIKTADVVITAIGKPHFFKTTNFKPGVILYDVGVSRNVLNKLCGDIDPNGIEKVAKWWSPTPGGVGPFTVLAIMKNLWVLYEAHQRRI" }, "paralog": false, "identity": 0.9851301115241635, "score": 536.0, "evalue": 8.12e-196, "description": "Internal stop codon at 18139, 18199, 18853. Nonsense mutation around 18139, 18199, 18853.", "psc": { "uniref90_id": "UniRef90_P75096", "gene": "folD", "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_P47259", "cog_id": "COG0190", "cog_category": "H", "go_ids": [ "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75096", "UniRef:UniRef50_P47259", "COG:COG0190", "COG:H", "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_P47259", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47259" ], "product": "Bifunctional protein FolD" } }, "id": "GIHJHMOALN_69", "locus": "GCA009939745_00027" }, { "type": "tRNA", "sequence": "contig_1", "start": 18998, "stop": 19073, "strand": "-", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 68.8, "nt": "GGGGATGTAGCTCAACTGATAGAGCACCTGATTTGCACTCAGGAGGTTGAGGGTTTGAGACCCTTCATCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 19038, 19040 ], "id": "GIHJHMOALN_36", "locus": "GCA009939745_00028" }, { "type": "tRNA", "sequence": "contig_1", "start": 19079, "stop": 19155, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 84.3, "nt": "GGAAGTATAGCTCAGTTGGTTAGAGCACACCCCTGATAAGGGTGAGGTCGATGGTTCAAGTCCATTTACTTCCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 19119, 19121 ], "id": "GIHJHMOALN_35", "locus": "GCA009939745_00029" }, { "type": "cds", "sequence": "contig_1", "start": 19249, "stop": 20004, "strand": "+", "frame": 1, "gene": "mdlB", "product": "Putative ABC transporter ATP-binding MG014-like protein", "db_xrefs": [ "COG:COG1132", "COG:V", "GO:0005524", "GO:0005886", "GO:0015421", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47260", "UniRef:UniRef90_P75095" ], "nt": "ATGGGTCTTGTTTTAAAACAATTTAACAGGAAAATAAGAACAGCTTTAATTCTGGCGCCGCTTTTCACCTTTGCACAGATTATTATTGATCTTATTATTCCGTCCTTTTTGGCCAGTGCTATTGCCGTAGTGTTCTCAATTGTCACGCTTAAACAAAAAGAAGCATCAGGCGAGGGAGTTGCTGTTGATTTTATTGCTGAAAGTAAGTTAAGTTTCCAAAGTGTCCAGGAAGCGCAAATAGTGTTAGCGACCTCAGTGATCTTGTTAGCACTCTTTGGTTTAGTGTTTGGGTTAATTTCCATTTTTTGTGCCAGCATAGTAGCGGGGAATACCTCTTACTTTTTAAGAAGGAAGATTTTTCGCAAGATTATGCACATTACCGCACCTTCTCATGACCAATATGGCTCTTCTACGTTGTTGGTAAGGTTGACCAATGACGTTTACTTAATGGAAATCATGACCTTTGATTTTTTGCGGTTAATTGTTCGTGCACCTTTCTTGTTTATAGGGGGTCTAGCCTTTGCTATTGCCACTAACTCTGACATGTCGATTTCGCTAGCCATTACCTTTCCTTTGACCTTCTTAGTGATTGGGATTCTCAACAAGAAATCGACCCCGTTATTTAAGAACAACCAGAAATCGGTGGACCAAATTAACGAACGCGTGGAGGAAGATGTGTCGGGTTACAAGGTAGTGCAGTCGTTTAACTTAAAGGACACGGAATGTCTCAAGTTTAAGGCAGCTAATGCAAGGTGA", "aa": "MGLVLKQFNRKIRTALILAPLFTFAQIIIDLIIPSFLASAIAVVFSIVTLKQKEASGEGVAVDFIAESKLSFQSVQEAQIVLATSVILLALFGLVFGLISIFCASIVAGNTSYFLRRKIFRKIMHITAPSHDQYGSSTLLVRLTNDVYLMEIMTFDFLRLIVRAPFLFIGGLAFAIATNSDMSISLAITFPLTFLVIGILNKKSTPLFKNNQKSVDQINERVEEDVSGYKVVQSFNLKDTECLKFKAANAR", "aa_hexdigest": "b14ef64c41b6695270d5f377985cf1b4", "start_type": "ATG", "rbs_motif": null, "genes": [ "mdlB" ], "truncated": "3-prime", "pseudogene": { "start": 19249, "stop": 20307, "observations": { "mutation": [ 20002 ], "stop": [ 20002 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75095", "query_cov": 1.0, "subject_cov": 0.4028892455858748, "identity": 1.0, "score": 454.0, "evalue": 1.78e-155, "gene_start": 1, "gene_end": 251, "reference_start": 1, "reference_end": 251, "reference_sequence": "MGLVLKQFNRKIRTALILAPLFTFAQIIIDLIIPSFLASAIAVVFSIVTLKQKEASGEGVAVDFIAESKLSFQSVQEAQIVLATSVILLALFGLVFGLISIFCASIVAGNTSYFLRRKIFRKIMHITAPSHDQYGSSTLLVRLTNDVYLMEIMTFDFLRLIVRAPFLFIGGLAFAIATNSDMSISLAITFPLTFLVIGILNKKSTPLFKNNQKSVDQINERVEEDVSGYKVVQSFNLKDTECLKFKAANARWTKSSTNSLFVNTLNIPFTFFFSSMTIIIALLLVFQLDNSVRVDPLPDNAAIRPSIFAFFQYNFYIVLGLILTSLTMVNFTRSRVALGRIKDVLNKPEIQQHVVPDQTVLAPSLEFKNVAFGLGNKENRDFLQDLNFKFEAGKTYGIVGPTGSGKSLIANIIGGLYEPNQGEIFVGGQSIKTIDSDYLAKMIGIVFQQNILFKGTIASNIKIGLETREDWKHEPDSKKDAAMKRAAAIACADTFIEKFSDTYDHTVEQLGKNLSGGQKQRVAIARTVITKPQILVLDDSMSALDALTEKKVRENIANELPGTTKIIISQNINSIKYAHKIMVIDNGRIAGFDSDAKLMQSCDIYVKMKQAQKDQGGDFDAVA" }, "paralog": false, "identity": 0.9971590909090909, "score": 662.0, "evalue": 1.63e-239, "description": "Internal stop codon at 20002. Nonsense mutation around 20002.", "psc": { "uniref90_id": "UniRef90_P75095", "gene": "MdlB", "product": "Putative ABC transporter ATP-binding protein MG014 homolog", "uniref50_id": "UniRef50_P47260", "cog_id": "COG1132", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75095", "UniRef:UniRef50_P47260", "COG:COG1132", "COG:V", "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47260", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47260" ], "product": "Putative ABC transporter ATP-binding protein MG014" } }, "id": "GIHJHMOALN_70", "locus": "GCA009939745_00030" }, { "type": "cds", "sequence": "contig_1", "start": 20026, "stop": 21120, "strand": "+", "frame": 1, "gene": "mdlB", "product": "Putative ABC transporter ATP-binding MG014-like protein", "db_xrefs": [ "COG:COG1132", "COG:V", "GO:0005524", "GO:0005886", "GO:0015421", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47260", "UniRef:UniRef90_P75095" ], "nt": "TTGTTTGTCAATACACTGAACATTCCCTTTACCTTCTTCTTTTCCAGTATGACTATCATCATTGCGTTGCTTTTAGTCTTCCAACTAGATAATTCAGTCAGAGTGGATCCCCTACCAGATAATGCTGCCATTAGACCCAGCATCTTTGCTTTCTTCCAGTACAACTTCTATATTGTCTTGGGTTTAATCTTGACCTCGCTAACAATGGTCAACTTTACCCGTTCCCGCGTGGCCTTGGGTCGGATTAAGGATGTTTTGAATAAACCGGAAATCCAACAACATGTGGTTCCTGATCAAACAGTTCTAGCCCCTAGCTTGGAATTCAAAAACGTGGCCTTTGGGTTAGGTAACAAGGAAAACCGGGACTTCTTGCAAGACCTTAACTTTAAGTTTGAGGCCGGTAAGACTTACGGAATTGTGGGACCTACTGGTTCGGGTAAGTCTTTAATAGCTAATATTATTGGGGGTTTATATGAACCCAACCAAGGTGAAATCTTTGTTGGTGGTCAAAGTATTAAAACGATTGACAGTGACTATTTAGCCAAGATGATCGGGATTGTCTTTCAGCAAAACATTCTCTTTAAGGGCACGATTGCTTCCAACATTAAGATAGGCCTGGAAACCAGGGAAGACTGGAAGCACGAACCAGATAGCAAAAAGGATGCTGCCATGAAACGCGCTGCTGCCATTGCTTGTGCTGATACCTTCATCGAAAAGTTCAGCGATACCTATGACCATACGGTGGAACAGTTAGGCAAAAACCTCTCTGGTGGGCAAAAACAACGCGTCGCCATTACTAGAACCGTTATTACTAAACCACAAATCTTAGTGTTGGATGACAGTATGAGTGCGCTGGACGCTTTAACCGAAAAGAAGGTCCGGGAAAACATTGCCAACGAACTTCAGGGCACCACTAAGATCATTATTAGTCAAAACATTAATTCGATTAAGTACGCGCACAAAATTATGGTGATTGATAACGGTCGGATTGCTGGGTTTGACAGTGATGCTAAGCTCATGCAGAGCTGTGACATCTATGTCAAGATGAAGCAAGCACAAAAAGACCAAGGAGGTGATTTCGATGCTGTCGCATAA", "aa": "MFVNTLNIPFTFFFSSMTIIIALLLVFQLDNSVRVDPLPDNAAIRPSIFAFFQYNFYIVLGLILTSLTMVNFTRSRVALGRIKDVLNKPEIQQHVVPDQTVLAPSLEFKNVAFGLGNKENRDFLQDLNFKFEAGKTYGIVGPTGSGKSLIANIIGGLYEPNQGEIFVGGQSIKTIDSDYLAKMIGIVFQQNILFKGTIASNIKIGLETREDWKHEPDSKKDAAMKRAAAIACADTFIEKFSDTYDHTVEQLGKNLSGGQKQRVAITRTVITKPQILVLDDSMSALDALTEKKVRENIANELQGTTKIIISQNINSIKYAHKIMVIDNGRIAGFDSDAKLMQSCDIYVKMKQAQKDQGGDFDAVA", "aa_hexdigest": "2c0af5880c98747cf8cfe0c07aea602b", "start_type": "TTG", "rbs_motif": null, "genes": [ "mdlB" ], "truncated": "5-prime", "pseudogene": { "start": 19726, "stop": 21120, "observations": { "mutation": [ 20002 ], "stop": [ 20002 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75095", "query_cov": 1.0, "subject_cov": 0.5842696629213483, "identity": 0.992, "score": 699.0, "evalue": 5.21e-250, "gene_start": 1, "gene_end": 364, "reference_start": 260, "reference_end": 623, "reference_sequence": "MGLVLKQFNRKIRTALILAPLFTFAQIIIDLIIPSFLASAIAVVFSIVTLKQKEASGEGVAVDFIAESKLSFQSVQEAQIVLATSVILLALFGLVFGLISIFCASIVAGNTSYFLRRKIFRKIMHITAPSHDQYGSSTLLVRLTNDVYLMEIMTFDFLRLIVRAPFLFIGGLAFAIATNSDMSISLAITFPLTFLVIGILNKKSTPLFKNNQKSVDQINERVEEDVSGYKVVQSFNLKDTECLKFKAANARWTKSSTNSLFVNTLNIPFTFFFSSMTIIIALLLVFQLDNSVRVDPLPDNAAIRPSIFAFFQYNFYIVLGLILTSLTMVNFTRSRVALGRIKDVLNKPEIQQHVVPDQTVLAPSLEFKNVAFGLGNKENRDFLQDLNFKFEAGKTYGIVGPTGSGKSLIANIIGGLYEPNQGEIFVGGQSIKTIDSDYLAKMIGIVFQQNILFKGTIASNIKIGLETREDWKHEPDSKKDAAMKRAAAIACADTFIEKFSDTYDHTVEQLGKNLSGGQKQRVAIARTVITKPQILVLDDSMSALDALTEKKVRENIANELPGTTKIIISQNINSIKYAHKIMVIDNGRIAGFDSDAKLMQSCDIYVKMKQAQKDQGGDFDAVA" }, "paralog": false, "identity": 0.9935344827586207, "score": 894.0, "evalue": 0.0, "description": "Internal stop codon at 20002. Nonsense mutation around 20002.", "psc": { "uniref90_id": "UniRef90_P75095", "gene": "MdlB", "product": "Putative ABC transporter ATP-binding protein MG014 homolog", "uniref50_id": "UniRef50_P47260", "cog_id": "COG1132", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75095", "UniRef:UniRef50_P47260", "COG:COG1132", "COG:V", "GO:0005886", "GO:0015421", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47260", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47260" ], "product": "Putative ABC transporter ATP-binding protein MG014" } }, "id": "GIHJHMOALN_71", "locus": "GCA009939745_00031" }, { "type": "cds", "sequence": "contig_1", "start": 21167, "stop": 21478, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGGTCGCACTTCCTCCAAGGTGCTGTATGTCATGGTCTGTGCCATCTTTGGTATTCTCACTGGGGTTACTAACTCGATTCTCTTGGCCCAGGGCCTGGGTTTTATTTTTCCTACCACTAATACCGAAACTGATGGTATCCAATCAGTGTACTTACTGGTATTTGCGCACAACTTACCGGTCATGGAGCGCTTGACGATTGTTTGTGTTACCGTGGTTGTGGCTTACATTCTCATCTTTAGTTTTAACGTAGCGCAGAACTATTTAGGTTTAAAGCTGTACCAAGAGATCTGTGCCTTGTTGCGCTGA", "aa": "MQGRTSSKVLYVMVCAIFGILTGVTNSILLAQGLGFIFPTTNTETDGIQSVYLLVFAHNLPVMERLTIVCVTVVVAYILIFSFNVAQNYLGLKLYQEICALLR", "aa_hexdigest": "31c83c977d03b970aba79cd36d9158f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11365.43409999999, "isoelectric_point": 7.711539268493653 }, "id": "GIHJHMOALN_72", "locus": "GCA009939745_00032" }, { "type": "cds", "sequence": "contig_1", "start": 21503, "stop": 22108, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCACTAGTTTCTTTGATACGCAGAACAACGGGGATTTAATGAGTCGCTTGACCAACGACGTTTACAACATTAACAACCTGTATGCCCAAGTGGGTGGACAGACAATTCAGAGTTTGTTTATCTTAATGACAACAGCGACGATTCTGTTTGTTTTAAGTCCAGTCATTGCCTTAATTTCGCTAACAGTCTTAATTGCTCTAATTGCCCTTTCCTTTCTCTTTTTAAAGAAGGCACGCGCTGCTTATGCAAAGGTGCAAAACAACTTAGGGGACATGTCGGGTTACATTGAAGAAGTGTTGTCGAACCATAAGGTAGTTCATGTCTTGAAACTGCAAGAGGTGATGATTGATAACTTTGACAAGTACAACCGCCCGATGGTTAACCCCACCATTAAGGCCAATACCTATGCGGTGTTTATTTACTCCTGGTTTGGTTTTATTTCCAATATTACTTACTTAGCTTCGATCTCAATTGCCACGGCCTTTAGTGTGAATAACATTCCCTCCTTTGGGGTGAGTGCGATTAACTATTCGTTTATGCTGTCTTACATTGCGGCCTTACGTCAAACAGCACTCCCTTTAAACCAGATCTTTAGCTTGTGA", "aa": "MSTSFFDTQNNGDLMSRLTNDVYNINNLYAQVGGQTIQSLFILMTTATILFVLSPVIALISLTVLIALIALSFLFLKKARAAYAKVQNNLGDMSGYIEEVLSNHKVVHVLKLQEVMIDNFDKYNRPMVNPTIKANTYAVFIYSWFGFISNITYLASISIATAFSVNNIPSFGVSAINYSFMLSYIAALRQTALPLNQIFSL", "aa_hexdigest": "cbb029256f4e08b4f3171f348f58c13d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22366.792800000003, "isoelectric_point": 8.803302955627444 }, "id": "GIHJHMOALN_73", "locus": "GCA009939745_00033" }, { "type": "cds", "sequence": "contig_1", "start": 22157, "stop": 22438, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGGATCTAGAAAGTCCCCAAAAACAGGCCACCATTACTAAGTTACCCAACATTAAGGGCAACATCCGTTTTGAAAAGGTAGCTTTTGGTTACAGTGCGGACAAACCAATTTTAACGGGGATTGATTTCTCGGTTAAACACGGTGACATTGTGGCCATAGTCGGTCCTACTGGAGCGGGTAAGTCCACCATTATTAACCTGTTGATGAAGTTCTACAAACCGTTTGCTGGGAAGATCTACATGGACAACTTTGAGATTAGTGAGGTGAGTGAAACTGCTTGA", "aa": "MDLESPQKQATITKLPNIKGNIRFEKVAFGYSADKPILTGIDFSVKHGDIVAIVGPTGAGKSTIINLLMKFYKPFAGKIYMDNFEISEVSETA", "aa_hexdigest": "b9822d01461eb319aa53df4a7546dea7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 51, "aa_cov": 0.5483870967741935, "hmm_cov": 1.0, "evalue": 2.4941737203987148e-14, "score": 49.54983139038086, "start": 38, "stop": 88 } ], "seq_stats": { "molecular_weight": 10167.684999999998, "isoelectric_point": 7.947614097595215 }, "id": "GIHJHMOALN_74", "locus": "GCA009939745_00034" }, { "type": "cds", "sequence": "contig_1", "start": 22457, "stop": 22936, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "GTGCTCCAAGACCCCTTCTTGTTTAGTGGCACGATTAAGGAAAACATCCGCATGGGACGGCAAGATGCCACTGATGAGGAAATTATAGAGGCGTGCAAGGTGGCCAATGCTCATGACTTTATTATGCGTTTACCCCAAGGTTACAACACCTTTATTTCCAACAAAACTGATTATCTCTCGGTCGGTGAGCGCCAACTGTTAACTATTGCCCGCGCTGTTATTCGTAATGCCCCGGTGTTATTATTGGATGAAGCCACGAGCTCCATCGATGTACACTCCGAAAAATTGATCCAACAATCAATAGGCAGACTAATGAAGGATAAAACATCGTTTATAATATCCCATCGACTCTCAATCATCCGTAATGCGACGCTGATAATTGTGATTAATGATGGAAAAGTTTTGGAAATGGGTAACCATGAACAATTAATGCGGCAAAACGGTTTTTATGCTCGATTAAAGCGTTCTGCTGTGAAGTAA", "aa": "MLQDPFLFSGTIKENIRMGRQDATDEEIIEACKVANAHDFIMRLPQGYNTFISNKTDYLSVGERQLLTIARAVIRNAPVLLLDEATSSIDVHSEKLIQQSIGRLMKDKTSFIISHRLSIIRNATLIIVINDGKVLEMGNHEQLMRQNGFYARLKRSAVK", "aa_hexdigest": "eec678936ab4730ac6829e1efe0efd98", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00307619A1", "query_cov": 0.9811320754716981, "subject_cov": 0.8082901554404145, "identity": 0.551, "score": 176.0, "evalue": 3.12e-53, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A354MUK2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 88, "aa_cov": 0.5345911949685535, "hmm_cov": 0.8181818181818182, "evalue": 1.5815036054912486e-11, "score": 40.47453308105469, "start": 3, "stop": 87 } ], "seq_stats": { "molecular_weight": 18043.70329999999, "isoelectric_point": 9.12822360992432 }, "id": "GIHJHMOALN_75", "locus": "GCA009939745_00035" }, { "type": "cds", "sequence": "contig_1", "start": 22946, "stop": 23320, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04434.22" ], "nt": "ATGACGATTGCTGAAATAAGGAAATTTGCACAAACCACGCAAAAGTTTGTCGAAGCGGAAAACCTGTTTGAGCATGGTAACGTAGTCTTGCCCAAAAAATACCTCAATAAGGCGCGGGGCATGGCTGAAGTATTGTACAACAGCCAGGTCATTAAAGTAAGTTTTACGGCTAAAGATGGTGAGCTAACGTGTAAGTGTAGTTGTTTGGCTAACGTTGACAACTGCGTGCACATTGTAGCTGTATTGCTCAAATACCACCAAATGCTAGTGGAGTCCAAACGCAGTTTTAACTTAGCTGAAGCCTTTCACTTGGACTGTGATCAGGCCGAAATGCTGATTGAAAACCTCAGTTTAGAGATTATTGCCGGGGGATGA", "aa": "MTIAEIRKFAQTTQKFVEAENLFEHGNVVLPKKYLNKARGMAEVLYNSQVIKVSFTAKDGELTCKCSCLANVDNCVHIVAVLLKYHQMLVESKRSFNLAEAFHLDCDQAEMLIENLSLEIIAGG", "aa_hexdigest": "1c9ab90bbbc57fb82fa825fca0a254e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04434.22", "name": "SWIM", "length": 29, "aa_cov": 0.20967741935483872, "hmm_cov": 1.0, "evalue": 6.300316425224494e-08, "score": 27.563405990600586, "start": 60, "stop": 85 } ], "seq_stats": { "molecular_weight": 13894.024299999988, "isoelectric_point": 6.063549613952636 }, "id": "GIHJHMOALN_76", "locus": "GCA009939745_00036" }, { "type": "cds", "sequence": "contig_1", "start": 23378, "stop": 24478, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCTTTTATTGTTGTGATGCGACCTTTGTGTACTTCCTTCACTTGGAAAACGACACTTTGCACTCAGTCGAATTGTCCAAGTTTAAACCAGAGGAACGCGCTACCTTACTCTTCTTTGATAAATTGTGCAAGCAGTTTACAGTGGGGTATGACCGCAACAGTTTGCTCTTTCCGTTAGCGGGTTTTTTAAAGGAGTTACAAGCCAACACGGAGCCCACCATCTTTGTCTTTAACGATGACAAAATTGACAACATCCTCTTTTTAAGGATTTCCAAAAAGCACCATGGTTTAAACCATGTTTGTGGTTTTAGTGGCAAGAAGGTATTTGACTTTGTTACTTATAAACAAAAGGAAAAGCAGATTGTGTTGCGCTCAGCGTACCTGAGTAAGTTCACTGACTTTAAGTTTCCTTACACCATTAACATCTACAAACTCCAGTTTGGGGAACCGCTGTTCTTTCACTTTTTAATTCAGCTAAAGCGTGATGGCTTTAAGAACTTCTACTTTCAAAGTGAAGACAGTATTGTCAAGGAAAAGGAATACCTACCGAAGCTGTACTTTAAGGTCGAGTACGATCCGGTCAAAAACAAGTTTGTCAGTGATGCCTTCTTTAAGTACAAAAACCACTTTAATAAGGGCACAACCACGGTGTATCCGCACCGCTATTACATGGCTAAAAAGTCGGATCGCGGTGGTTTTAACCGCTTGTTGTTTTACGAAGAGGCCGTGGAAAACTTCTACCAAGACCAGTTTGATTTGGGTTACTTCCGTAAGTTTGAACACCTACCGATCCAGGACAAAAACCAGATTGAGGCCTTTAAAGCAGCGCTAGATGACTTAATGCCCGTGGATTTGGCTGAAGTGAGTTTATCCGATAACTTACTCCACCAAAAACCGCTTCATTTTGCCCTGTCTGATCTAGAAGCAGTTGCTGTTGATGACAAACAAATTAAGTTAAGTTTTGCCCCTAGTGCGGTTGAACTGAAATTAATTAAACGGATCTTATCCGCTTACCATAAGGGCAATGTGGTCTGTATCGACCAGGAGAGTTGGTATGACCTCAAGCAACCAGCTGCTAAAGAACTAATCCAGTTCTGA", "aa": "MRFYCCDATFVYFLHLENDTLHSVELSKFKPEERATLLFFDKLCKQFTVGYDRNSLLFPLAGFLKELQANTEPTIFVFNDDKIDNILFLRISKKHHGLNHVCGFSGKKVFDFVTYKQKEKQIVLRSAYLSKFTDFKFPYTINIYKLQFGEPLFFHFLIQLKRDGFKNFYFQSEDSIVKEKEYLPKLYFKVEYDPVKNKFVSDAFFKYKNHFNKGTTTVYPHRYYMAKKSDRGGFNRLLFYEEAVENFYQDQFDLGYFRKFEHLPIQDKNQIEAFKAALDDLMPVDLAEVSLSDNLLHQKPLHFALSDLEAVAVDDKQIKLSFAPSAVELKLIKRILSAYHKGNVVCIDQESWYDLKQPAAKELIQF", "aa_hexdigest": "8a4437a313ec6a2557d743580948e873", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 43257.24040000007, "isoelectric_point": 8.32223987579346 }, "id": "GIHJHMOALN_77", "locus": "GCA009939745_00037" }, { "type": "cds", "sequence": "contig_1", "start": 24551, "stop": 24709, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCAAGATCTTTAGTCAGTACTTGGACATTAAAAACCTGTTTGATGTACCGACCATTAAAAAGATTGAAGACCAAAACAACAACGTCTTTGACCTTTCCTTAGAACACAAAAAGATTACTTCACTGCGCAACTACCAACAAGAAGGAGTGAAGTGA", "aa": "MAKIFSQYLDIKNLFDVPTIKKIEDQNNNVFDLSLEHKKITSLRNYQQEGVK", "aa_hexdigest": "7fcdafb0b2a1e95f22e7e76ad5f862b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6155.9847, "isoelectric_point": 8.029811286926268 }, "id": "GIHJHMOALN_78", "locus": "GCA009939745_00038" }, { "type": "cds", "sequence": "contig_1", "start": 24719, "stop": 24871, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00176.29" ], "nt": "TTGGAGGAAAATAAGTTTGGTGGTATTCTGGCTGACGAAATGGGGTTAGGGAAAACGGTGCAGGTGATCTTTGCGCTGTTGGACAGCTATCTCAAAAACCACGTTAACCTCCCTAGCTTAATTATTGTTCCGGCTTCTTTATTGTTGAACTGA", "aa": "MEENKFGGILADEMGLGKTVQVIFALLDSYLKNHVNLPSLIIVPASLLLN", "aa_hexdigest": "06aeeb07d933bf4c611fa98f18b6cd66", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 53, "aa_cov": 0.96, "hmm_cov": 0.9811320754716981, "evalue": 3.7715810826349503e-13, "score": 44.63841247558594, "start": 2, "stop": 49 } ], "seq_stats": { "molecular_weight": 5440.376199999999, "isoelectric_point": 4.895849418640137 }, "id": "GIHJHMOALN_79", "locus": "GCA009939745_00039" }, { "type": "cds", "sequence": "contig_1", "start": 24947, "stop": 25171, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00176.29" ], "nt": "TTGTACGAAAAACTTACTAACGAAATCCTGATTGTGAGCTTTAACGTGTTGCGCAGTGATGTGAAGTTAATTACCAAACAGCGCTTTCACTATGTTGTAATTGACGAAGCGCAGGGCATTAAAAACGACAGCTCTTCAATTACCAAAGCTGCCAAAAAGGTGAAGGGCAACTTCTGTTTGGCCTTAACTGGTACGCCAATAGAAAACCGCTTGTTGGACCTTTGA", "aa": "MYEKLTNEILIVSFNVLRSDVKLITKQRFHYVVIDEAQGIKNDSSSITKAAKKVKGNFCLALTGTPIENRLLDL", "aa_hexdigest": "2f074a5cf1e1038ab1f889e593fc6949", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 69, "aa_cov": 0.9324324324324325, "hmm_cov": 1.0, "evalue": 4.261339870727223e-17, "score": 57.59275817871094, "start": 6, "stop": 74 } ], "seq_stats": { "molecular_weight": 8353.668099999999, "isoelectric_point": 9.206359291076659 }, "id": "GIHJHMOALN_80", "locus": "GCA009939745_00040" }, { "type": "cds", "sequence": "contig_1", "start": 25187, "stop": 25822, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37", "PFAM:PF00176.29" ], "nt": "GTGCTCCCAAGCTTTTTAGGGAACAAAAAGCAGTTTACCGACCAGTTTGAAAAGGAAAAAACCGACCAAAGCTTCCATTTGTTAATGCAGCGCACTAGTCCCTTTATTTTGCGTCGTACCAAGTCCAAGGTCTTGAAGGAACTGCCTAACAAAATTACCACCGATATTTACGTAGAATTAAACCCAATGCACCAGAAGCTGTATGAGGAAGAACGTGACCGCGGTTTGGAGGAAATTAAGCAAATCCAAGACAAGAGTTCCTTTAACATCTTGACTTTAATCTTGAAGTTAAGGCACTTGTGTAGTTTACCCAAAAACAGTCAGGGTATTTTGGAAAACAGTGCCAAAAAGGAAGCTGCTTTAGAAATTATCCACGAAGCGATTGAAAACCAGCGCAAGATTATCCTCTTTACCCAGTTCATTGATGTAATTGACCACTTTAAAGACACCTTTAAAGAGCAGGGCATTGAGTACTTTATCTTTGATGGCCGTAAGTCTCCTAAAAGCCGTCACAGTATTATCGAGAAGTTCAACAATGCCAAAAACCCTTGTGTGTTGTTAGCGTCCTTGAAAGCAGGGGGTGTGGGAATTAACCTCACTGCCGCTGAAGTGGTAATCCACTTTGATGTGTGGTGA", "aa": "MLPSFLGNKKQFTDQFEKEKTDQSFHLLMQRTSPFILRRTKSKVLKELPNKITTDIYVELNPMHQKLYEEERDRGLEEIKQIQDKSSFNILTLILKLRHLCSLPKNSQGILENSAKKEAALEIIHEAIENQRKIILFTQFIDVIDHFKDTFKEQGIEYFIFDGRKSPKSRHSIIEKFNNAKNPCVLLASLKAGGVGINLTAAEVVIHFDVW", "aa_hexdigest": "fbae1f9d8d0b765c8039e2539f9645fd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 94, "aa_cov": 0.44075829383886256, "hmm_cov": 0.9468085106382979, "evalue": 1.1630354179776035e-18, "score": 62.93749237060547, "start": 119, "stop": 211 }, { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 109, "aa_cov": 0.4834123222748815, "hmm_cov": 1.0, "evalue": 3.973813783291712e-14, "score": 47.84605407714844, "start": 3, "stop": 104 } ], "seq_stats": { "molecular_weight": 24498.081100000018, "isoelectric_point": 8.939589118957521 }, "id": "GIHJHMOALN_81", "locus": "GCA009939745_00041" }, { "type": "cds", "sequence": "contig_1", "start": 25832, "stop": 26038, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAACCAAGCGACTGACCGGGCCCACCGCATAGGACAAAAGAAAACAGTCCAAGTTTACCGCATTATTGCTAAAAATACGATTGAAGAGCGGGTCTGTCAAGTGCAGGCCGAAAAACAGGAGTTAGTGAGCAAAACGCTAGTGGAAGACGTCAATTTTTTCGAATCCTTGACCAATGAGGAATTATTACGTCTTTTTGAATAA", "aa": "MENQATDRAHRIGQKKTVQVYRIIAKNTIEERVCQVQAEKQELVSKTLVEDVNFFESLTNEELLRLFE", "aa_hexdigest": "541de4aabdea3cbc664f5d836f4aae02", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8009.008499999999, "isoelectric_point": 5.4145620346069325 }, "id": "GIHJHMOALN_82", "locus": "GCA009939745_00042" }, { "type": "cds", "sequence": "contig_1", "start": 26084, "stop": 27256, "strand": "+", "frame": 2, "gene": "dnaJ", "product": "Chaperone protein DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085", "RefSeq:WP_014325289.1", "SO:0001217", "UniParc:UPI0002386D06", "UniRef:UniRef100_A0AB33HMG6", "UniRef:UniRef50_P78004", "UniRef:UniRef90_P78004" ], "nt": "ATGGCCGCTGGTAAACGTGATTATTATGAAGTGTTAGGCGTTTCCAGATTCGCCACCGCACAAGACATCAAACGAGCTTTCCGCAAGCTTGCTATGCAGTACCACCCCGACCGCCATAAGGGTGAGGGTGAGACTGTCCAAAAACAAAACGAAGAGAAGTTTAAAGAGGTCAATGAGGCGTACGAGGTCTTAAGTGACACCGAGAAGCGTGGTATGTACGACCGCTTTGGCCATGAAGGGTTAAATGCCTCTGGCTTCCATGAAACTGGGTTTAATCCCTTTGACATCTTTAACAGTGTCTTTGGTGAGGGCTTTTCGTTTGACATGGACGGGGGTTCACCGTTTGACTTTATCTTTAGTAGGGGCAAAAAGTCGCGGCAAAACCGGGTTGTTTTGCCCTATGATTTAGAGATTGCGGTGGGGGTAGACATTAGCTTCTTTGAGATGACTAATGGTTGTACTCGGACGATTGAATATACCAAACGGGTGACCTGTAGTGCCTGTGATGGTTTTGGGGCTGAAGGGGGAGAAACCGGGATGGTTAGCTGTAACAGTTGTGAAGGCAATGGTTTTATTTTGAAGAATCAACGCTCCATCTTTGGTACAGTCCAGTCCCAGATGTTGTGTCAGAGCTGTGGTGGTCAGGGTAAACAGGCCAAACACAAGTGTAAGACTTGTAAGGGAAGTAAGTACAAGAAGGTACCAACTACCAAGGAAATTCAGATTCCCGCTGGCATTTACCATGGTGATGCGTTAGTCGATGACCATGGTGGTAATGAATTCGGGGGTCACGTTGGCAAGTTAGTGGTCCATGTGGGGGTACTACCGAGTAAGATCTTTAAACGGGTTGACCATAATGTAGTAGCCAATGTTTTAGTCGATCCGATGATTGCTATCACCGGAGGGAAAATTGAACTCCCTACCTTAGAAGGCATTAAGGAATTCAACTTACGTGCTGGTACTAAGAGTGGCGAGCAAATAGTAATCCCTGGTGGTGGGATTAAATTTACGAAGAACTTCCGCAAGAAGGCCGGGGATCTCATTATTATTATCAGTTATGCCCGTCCAAGTGAATACAGTGCGCCAGAGTTGCGCAAGCTCAAGGAATTTATTAAACCTAATCAAGAGGTAAAACAATATTTAAACGCACTTAAGAATGAATACAAGTCCTAA", "aa": "MAAGKRDYYEVLGVSRFATAQDIKRAFRKLAMQYHPDRHKGEGETVQKQNEEKFKEVNEAYEVLSDTEKRGMYDRFGHEGLNASGFHETGFNPFDIFNSVFGEGFSFDMDGGSPFDFIFSRGKKSRQNRVVLPYDLEIAVGVDISFFEMTNGCTRTIEYTKRVTCSACDGFGAEGGETGMVSCNSCEGNGFILKNQRSIFGTVQSQMLCQSCGGQGKQAKHKCKTCKGSKYKKVPTTKEIQIPAGIYHGDALVDDHGGNEFGGHVGKLVVHVGVLPSKIFKRVDHNVVANVLVDPMIAITGGKIELPTLEGIKEFNLRAGTKSGEQIVIPGGGIKFTKNFRKKAGDLIIIISYARPSEYSAPELRKLKEFIKPNQEVKQYLNALKNEYKS", "aa_hexdigest": "7183c5e4fb58711c14e99e19d2e31936", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386D06", "ncbi_nrp_id": "WP_014325289.1", "uniref100_id": "UniRef100_A0AB33HMG6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386D06", "RefSeq:WP_014325289.1", "UniRef:UniRef100_A0AB33HMG6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HMG6", "uniref90_id": "UniRef90_P78004" }, "psc": { "uniref90_id": "UniRef90_P78004", "gene": "dnaJ", "product": "Chaperone protein DnaJ", "uniref50_id": "UniRef50_P78004", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0031072", "GO:0051082", "GO:0008270", "GO:0051085", "GO:0006260", "GO:0042026", "GO:0009408" ] }, "pscc": { "uniref50_id": "UniRef50_P78004", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78004" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "GIHJHMOALN_83", "locus": "GCA009939745_00043" }, { "type": "cds", "sequence": "contig_1", "start": 27417, "stop": 28169, "strand": "+", "frame": 3, "gene": "pip", "product": "prolyl aminopeptidase", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.4.11.5", "GO:0004177", "GO:0005737", "GO:0006508", "GO:0030163", "SO:0001217", "UniRef:UniRef50_P75092", "UniRef:UniRef90_P75092" ], "nt": "TTGTTAGACCAACGGGGTTGTGGTCAAAGTAAAGCGGTTAATCCCTTACTGCACAACACTACCCAAGACTTAGTTGGGGACTTGGAAGCGTTGCGCCAACACTTAAAACTTGAGCGTTGGACCCTATTTGGTGGTAGTTGGGGTTCTACTTTAGCCTTAGTGTATGCCATTACCCATCCCCAAGTAGTCGAACAAGTCTTTTTAAGAGCGCTCTTTTTGGGCCGTGAACAAGACTGGGCTGAAATGCTGTTGGGCTTGGGCAAGCTCTTCTACCCTTACGAACACCAAACGTTACTGAAAGCCATTCCCCAGGCTTGTCGCACTGACTTTACGAAATTCACTAACTACTTTTATGAAGTGTTGCAAGGTAACGATTCCGCTTTAAAAACGCAGTTAGCCAACGCTTGGGTTAAGTGGGAAAATACCTTACTATCACCGATTAGCTATGTCAAAGACGAGAAAGCAGAAGATGCGAACTTTACCTTTAAGTTAGCGCTCTTGGAATGTCACTACGCAAAGCACCACAGCTTTTTAAAACCGAACTTTATCCTGGAAAATGTTGCGGTATTAAAGGACAAACCGGTGCACTTAATCCACGGCCGTTTTGATCTAGTCTGTCCGTTAAGTCAAGCCTTGGAACTCAAACGTGCTTTACCAACGCTTAACTTGTATGTAACTAACAATGCCGGGCACAGTGGCAGTGATCCCAATAACCTCACTACCATTAAACACCTTTTAAAAACACAACTGTAA", "aa": "MLDQRGCGQSKAVNPLLHNTTQDLVGDLEALRQHLKLERWTLFGGSWGSTLALVYAITHPQVVEQVFLRALFLGREQDWAEMLLGLGKLFYPYEHQTLLKAIPQACRTDFTKFTNYFYEVLQGNDSALKTQLANAWVKWENTLLSPISYVKDEKAEDANFTFKLALLECHYAKHHSFLKPNFILENVAVLKDKPVHLIHGRFDLVCPLSQALELKRALPTLNLYVTNNAGHSGSDPNNLTTIKHLLKTQL", "aa_hexdigest": "c9361cf0dd788333a54a3bbd304fb246", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75092", "query_cov": 1.0, "subject_cov": 0.8090614886731392, "identity": 0.996, "score": 513.0, "evalue": 2.13e-183, "valid": true, "gene": "pip", "product": "prolyl aminopeptidase", "ec_ids": [ "3.4.11.5" ], "uniref50_id": "UniRef50_P75092", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0004177", "GO:0005737", "GO:0006508", "GO:0030163" ] }, "pscc": { "uniref50_id": "UniRef50_P75092", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75092" ], "product": "Putative proline iminopeptidase" }, "genes": [ "pip" ], "id": "GIHJHMOALN_84", "locus": "GCA009939745_00044" }, { "type": "cds", "sequence": "contig_1", "start": 28169, "stop": 28411, "strand": "+", "frame": 2, "gene": null, "product": "Methionine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M6W2H4" ], "nt": "ATGAAACGGTGTTACATTACCACCCCGATTTACTACGCCTCGGGTAAGCCCCACATTGGCCATGCCTTTACGACCATCTTAGCGGATGTGATTAAGCGGTATAAACAGCAAAACGGTTATGAAGCTTACTTTCTAACTGGTACGGATGAACACGGCAACAAAATTGAAAGTAAGGCCAAAAGTTTAGGTCTAGATCCCCAAACCTTTGTGGATCAAAACGTGGCTTACTTTCAACAAATGTGA", "aa": "MKRCYITTPIYYASGKPHIGHAFTTILADVIKRYKQQNGYEAYFLTGTDEHGNKIESKAKSLGLDPQTFVDQNVAYFQQM", "aa_hexdigest": "e42754332f3ed166bcbd7561bddc9cc2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003620B786", "query_cov": 0.8375, "subject_cov": 0.9054054054054054, "identity": 0.5820000000000001, "score": 90.5, "evalue": 2.34e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A2M6W2H4" }, "pscc": { "uniref50_id": "UniRef50_A0A2M6W2H4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2M6W2H4" ], "product": "Methionine--tRNA ligase" }, "genes": [], "id": "GIHJHMOALN_85", "locus": "GCA009939745_00045" }, { "type": "cds", "sequence": "contig_1", "start": 28589, "stop": 28987, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTGTGAAATAGGTCACCAGTTAACCCTAGTCCAAGAACCATCTTTATTTATTGCCTTTAAAGATAGTAAGGACTGGATTGGGGAAATGATTGCGACAAACAAGCTCAACATTACCCCTGAATCCAGAGCCGCTGAGTTAAAGAACAACTTCTTGGATGGGGGTTTAAATGACTTAGCTTTAACACGGCAAAACGTGACTTGGGGAATTCCCGTACCCTTTGACAACAAGCAAACGATTTACGTGTGGTTTGATGCCTTGTTTAACTACATTACTAACTTAGGGTTTGCCCATAACGACCCTAAATTTAACAAATGGTGGAACAATAATGATGAGGAACACGAAGTCATTCATTTAATTTCACGCGAAATTACCCGTTTCCACTGCATTTACTGA", "aa": "MLCEIGHQLTLVQEPSLFIAFKDSKDWIGEMIATNKLNITPESRAAELKNNFLDGGLNDLALTRQNVTWGIPVPFDNKQTIYVWFDALFNYITNLGFAHNDPKFNKWWNNNDEEHEVIHLISREITRFHCIY", "aa_hexdigest": "6c8fca86a153831eb582f9744a053720", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15439.314699999992, "isoelectric_point": 5.234893989562988 }, "id": "GIHJHMOALN_86", "locus": "GCA009939745_00046" }, { "type": "cds", "sequence": "contig_1", "start": 29006, "stop": 29380, "strand": "+", "frame": 2, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310053", "UniRef:UniRef90_UPI0013310053" ], "nt": "TTGGGTTTTAAACTACCGACCCAGTTTCTCTCCCATGGTTGGATTGTCGATGGTAATGGGCACAAGATGTCCAAGTCGTTGGGCAATGTCATTTCCCCCGAAGAACTATTGGCCCAGTTTGGTGTTGATGGTACGCGTTATTGCTTACTTAAAGAAATGCGCTTGGACAAGGACAACCGCTGTAGTATGGCGATCTTTAAGGACATTTACAACGCTGATTTGGCCAACAGTTTTGGTAACCACGCATCACGTACCTTTGGCATGATCAAAAAGTACTTGGGCGGCCAGTTAGACTTTATCGAAGTCCAAGACCCTCAAGTAAAGCAGCTAATGGACCAAGCGGACCAAGCGATGGTTCAGTTTGACACCGCTTGA", "aa": "MGFKLPTQFLSHGWIVDGNGHKMSKSLGNVISPEELLAQFGVDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHASRTFGMIKKYLGGQLDFIEVQDPQVKQLMDQADQAMVQFDTA", "aa_hexdigest": "310817fc2d67953a17079e2085406933", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 28922, "stop": 29683, "observations": { "mutation": [ 28985, 29378 ], "stop": [ 28985, 29378 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013310053", "query_cov": 1.0, "subject_cov": 0.47509578544061304, "identity": 0.992, "score": 252.0, "evalue": 5.65e-83, "gene_start": 1, "gene_end": 124, "reference_start": 29, "reference_end": 152, "reference_sequence": "DEEHEVIHLISREITRFLCIYWPIFLHQLGFKLPTQFLSHGWIVDGNGHKMSKSLGNVISPEELLAQFGVDGTRYCLLKEMRLDKDNRCSMAIFKDIYNADLANSFGNHASRTFGMIKKYLGGQLDFIEVQDPQVKQLMDQADQAMVQFDTAWNNFQFYKGINGLLQLVFQASKLIDQLKPWELVKQTDYTLLKQLLFACVRCTQVCFVLLAPILVHTSTQIFDLFNFSAQARSKTHLADPQQLQKISLAPVIQLLFKRLD" }, "paralog": false, "identity": 0.9881422924901185, "score": 502.0, "evalue": 4.83e-184, "description": "Internal stop codon at 28985, 29378. Nonsense mutation around 28985, 29378.", "psc": { "uniref90_id": "UniRef90_UPI0013310053", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI0013310053", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013310053", "UniRef:UniRef50_UPI0013310053" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013310053", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310053" ], "product": "class I tRNA ligase family protein" } }, "id": "GIHJHMOALN_87", "locus": "GCA009939745_00047" }, { "type": "cds", "sequence": "contig_1", "start": 29537, "stop": 29707, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGCTTTGTCTTGTTAGCGCCGATCTTAGTTCATACCAGTACCCAGATATTTGACCTGTTTAACTTCAGTGCCCAAGCACGTAGTAAGACCCACCTAGCTGATCCACAACAACTGCAAAAAATCAGCTTAGCACCTGTAATTCAACTTTTATTTAAAAGATTGGATTAA", "aa": "MCFVLLAPILVHTSTQIFDLFNFSAQARSKTHLADPQQLQKISLAPVIQLLFKRLD", "aa_hexdigest": "8bfaafa6e1e3a50befcb53a9eba3718f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6367.485500000001, "isoelectric_point": 9.186051750183104 }, "id": "GIHJHMOALN_88", "locus": "GCA009939745_00048" }, { "type": "cds", "sequence": "contig_1", "start": 29728, "stop": 29826, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTATGCAAGTAGAATACCTCGATCTCATCTCCCAAGCGAAGGAGATTGCTGAGACCCAATTTAAGGCGGAACCGTTTTCCTTTGATGCTATTTGA", "aa": "MTMQVEYLDLISQAKEIAETQFKAEPFSFDAI", "aa_hexdigest": "6974cd282a195b65a38f65d1f70d5bb3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3695.1731, "isoelectric_point": 4.07884578704834 }, "id": "GIHJHMOALN_89", "locus": "GCA009939745_00049" }, { "type": "cds", "sequence": "contig_1", "start": 29836, "stop": 30168, "strand": "+", "frame": 1, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "COG:COG3343", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355", "KEGG:K03048", "SO:0001217", "UniRef:UniRef50_P47268", "UniRef:UniRef90_P75090" ], "nt": "GTGAAGCACTTTAAGATCTCCAAACAAGACGAACCTAACCTAATTTCGCGGTTTTACCAAGACTTTTTGGAAGACCCTAACTTTGTTTATTTAGGGGAACGCAACTGGAAGTTAAGGGACTTCATGAAGTTTGATGAATGGAACAAGATTTCCCAAGCAATGTTTGTGACCAAGGAAATCTTTGAAGAGGGTTATGAAGACCTGTCCAACAAGAAGAAGGAAAACGAGGAAGAAGTGAATGACTTCATTATGGGCAATGATGGTGATGACAACAGTACCGGTGATGAAATTGTGCAAGGACTCATTAACGATTTTCGCGACGATAAACAATAA", "aa": "MKHFKISKQDEPNLISRFYQDFLEDPNFVYLGERNWKLRDFMKFDEWNKISQAMFVTKEIFEEGYEDLSNKKKENEEEVNDFIMGNDGDDNSTGDEIVQGLINDFRDDKQ", "aa_hexdigest": "5ab03db53a56f21d3b2be325db4422cc", "start_type": "GTG", "rbs_motif": null, "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 29728, "stop": 30168, "observations": { "mutation": [ 29824 ], "stop": [ 29824 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75090", "query_cov": 1.0, "subject_cov": 0.7534246575342466, "identity": 0.991, "score": 224.0, "evalue": 6.5e-74, "gene_start": 1, "gene_end": 110, "reference_start": 37, "reference_end": 146, "reference_sequence": "MTMQVEYLDLISQAKEIAETQFKAEPFSFDAIWKEVVKHFKISKQDEPNLISRFYQDFLEDPNFVYLGERNWKLRDFMKFDEWNKISQAMFVTKEIFEEGYEDLSNKKKENEEEVNDFIMGNDGDDNSTGDEIVQGLINDFRDDKQ" }, "paralog": false, "identity": 0.9931506849315068, "score": 296.0, "evalue": 1.25e-104, "description": "Internal stop codon at 29824. Nonsense mutation around 29824.", "psc": { "uniref90_id": "UniRef90_P75090", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47268", "cog_id": "COG3343", "cog_category": "K", "kegg_orthology_id": "K03048", "go_ids": [ "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75090", "EC:2.7.7.6", "UniRef:UniRef50_P47268", "COG:COG3343", "COG:K", "KEGG:K03048", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47268", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47268" ], "product": "Probable DNA-directed RNA polymerase subunit delta" } }, "id": "GIHJHMOALN_90", "locus": "GCA009939745_00050" }, { "type": "cds", "sequence": "contig_1", "start": 30168, "stop": 30719, "strand": "+", "frame": 3, "gene": null, "product": "Fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "nt": "ATGCTAGTAAACATCAAACAAATGTTGCAACACGCTAAGCAACACCACTATGCTGTACCCCACATTAATATCAATAACTATGAATGGGCTAAGGCAGTATTAACAGCGGCTCAGCAGGCCAAGAGTCCGATTATTGTCTCCACTTCTGAGGGCGCTTTAAAGTATATGTCCGGACACCAAGTGGTAGTACCGATGGTAAAGGGCTTAGTCGATGCTTTAAAGATTACTGTACCCGTAGCACTCCACTTAGACCATGGTAGTTATGAAGGTTGTAAGGCAGCGTTGCAAGCTGGGTTTAGTTCCATTATGTTTGATGGCTCGCATTTACCCTTTCAAGAAAACTTCACTAAATCCAAGGAGTTAATTGAACTAGCTAAACAAACCAATGCTTCTGTGGAACTAGAAGTGGGTACCTTAGGTGGGGAAGAAGATGGTATTGTGGGTCAAGGTGAATTGGCCAACATTGAAGAGTGCAAGCAAATTGCGACCTTAAAACCGGATGCCTTAGCTGCTGGGATTGGTAACATCCACGGGCTTTACCCCGACAACTGA", "aa": "MLVNIKQMLQHAKQHHYAVPHININNYEWAKAVLTAAQQAKSPIIVSTSEGALKYMSGHQVVVPMVKGLVDALKITVPVALHLDHGSYEGCKAALQAGFSSIMFDGSHLPFQENFTKSKELIELAKQTNASVELEVGTLGGEEDGIVGQGELANIEECKQIATLKPDALAAGIGNIHGLYPDN", "aa_hexdigest": "d177b04684ec03c182927d8ac06c541e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FEF401E", "query_cov": 0.9016393442622951, "subject_cov": 0.9375, "identity": 0.636, "score": 212.0, "evalue": 1.69e-67, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A3B0P7V3", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P7V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "product": "Fructose-bisphosphate aldolase" }, "genes": [], "id": "GIHJHMOALN_91", "locus": "GCA009939745_00051" }, { "type": "cds", "sequence": "contig_1", "start": 30777, "stop": 31034, "strand": "+", "frame": 3, "gene": null, "product": "fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "nt": "GTGCTCCATGGTGGTTCGGGAATTCCGGAAGCGGATGTCAAAAAAGCAATTGGCTTAGGCATTAGCAAGCTCAACATTAACACTGAATGTCAACTAGCGTTTGCTAAAGCGATTCGGGAATATGTGGAAGCCAAGAAGGACTTAGATACCCACAATAAGGGCTATGACCCACGGAAATTGTTAAAGTCACCTACCCAAGCAATTGTGGACTGCTGCTTGGAAAAGATGCAGCTCTGTGGTTCAACCAATAAGGCTTAG", "aa": "MLHGGSGIPEADVKKAIGLGISKLNINTECQLAFAKAIREYVEAKKDLDTHNKGYDPRKLLKSPTQAIVDCCLEKMQLCGSTNKA", "aa_hexdigest": "75143f24b9163c163db3272e7b8045dc", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A097ST37", "query_cov": 1.0, "subject_cov": 0.8585858585858586, "identity": 0.647, "score": 119.0, "evalue": 2.17e-33, "valid": false, "product": "fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A097ST37", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A097ST37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "product": "fructose-bisphosphate aldolase" }, "genes": [], "id": "GIHJHMOALN_92", "locus": "GCA009939745_00052" }, { "type": "cds", "sequence": "contig_1", "start": 31035, "stop": 32015, "strand": "+", "frame": 3, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P37518", "UniRef:UniRef90_P75088" ], "nt": "ATGTTAAGTGCGGGAATTGTTGGTTTACCCAACGTTGGTAAATCAACCTTGTTTAGTGCTATTACCAACCTGCAAGTGGAAATAGCTAACTATCCCTTTGCCACGATTGAACCGAATGCCGGGATAGTTAACGTAATCGATGAGCGCTTAGACAAACTAGCGAGTTTAATTAAACCCGATAAGGTTACCCACACTACCTTTCGCTTTGTTGACATTGCTGGTTTAGTTAAGGGTGCCAGTAAGGGAGAAGGACTGGGCAACCAGTTCTTGGCCAACATCAGAGAAGTTGACCTTATTTGTCACGTAGTGCGTTGCTATGAGGACAAGAAGATTGTCCACGTTAATAACCAGGTCGACCCCGTATTTGACTTTGAAATTATTGTCAATGAGTTAATCCAGGCCGACATTGAGGTGGTGAATACCCGGATTGGCAAGATTAAGCGCAAGGCCGAATCTGGTGATAAGCAGTCCAAGGAAGAATATCAACTGCTAGCACCCGTTTTACAAGGTTTGCAACAAAACCAGATGGTGTTGCATCTAGTGAATGAAGTAGATTTAAAGAAACTCAAGTCACTTAATTTACTAACCGCCAAACCGATCCTAGTGGTGGCCAATGTGTCAGAAGCGGATTTGTCCAACCTTGACCATAATCCCCACCTCACTCAGCTCAACCAGTTCTTAAAACAACATAACTTACCCCATGCCATTCCTGTTTGTGCATTATTAGAAAATGAGTTGAGTAGCTTGGATGCCAATGGTCGTCAAGACTGGCTCAAGGAGTTGGGTTTAAGTGATTACCAAGGTTTAAACCAACTAATTAAAACGGCTTATGATGCGATTGGTCTGTGGTCATTCTTTACCTTTGGTAAGCAAGAAGTGCGCGCTTGGGCTTTTAAAAAGGGTTGGTTAGCACCGCAGTGTGCGGGTGAAATTCACACTGACTTTGAACGTGGTTTTATTAAAGTAGAAGTAATTAGTTGA", "aa": "MLSAGIVGLPNVGKSTLFSAITNLQVEIANYPFATIEPNAGIVNVIDERLDKLASLIKPDKVTHTTFRFVDIAGLVKGASKGEGLGNQFLANIREVDLICHVVRCYEDKKIVHVNNQVDPVFDFEIIVNELIQADIEVVNTRIGKIKRKAESGDKQSKEEYQLLAPVLQGLQQNQMVLHLVNEVDLKKLKSLNLLTAKPILVVANVSEADLSNLDHNPHLTQLNQFLKQHNLPHAIPVCALLENELSSLDANGRQDWLKELGLSDYQGLNQLIKTAYDAIGLWSFFTFGKQEVRAWAFKKGWLAPQCAGEIHTDFERGFIKVEVIS", "aa_hexdigest": "3c04210cf453a8bcfb7d85eba911b15b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75088", "query_cov": 1.0, "subject_cov": 0.8907103825136612, "identity": 1.0, "score": 637.0, "evalue": 6.5e-230, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P37518", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P37518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P37518" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "GIHJHMOALN_93", "locus": "GCA009939745_00053" }, { "type": "cds", "sequence": "contig_1", "start": 32260, "stop": 32349, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAACCAACTAAGAACCTCACCGTTAACCTAATTAACATTGGTTTTGATGCCTATTCCGACAGTCAAAAAGCGCTGTTAGCTTGA", "aa": "MLKPTKNLTVNLINIGFDAYSDSQKALLA", "aa_hexdigest": "969b4bf44870ad7e8a75185f124772ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3179.6838, "isoelectric_point": 8.18930606842041 }, "id": "GIHJHMOALN_94", "locus": "GCA009939745_00054" }, { "type": "cds", "sequence": "contig_1", "start": 32539, "stop": 32949, "strand": "-", "frame": 1, "gene": "rimL", "product": "putative protein MPN_027", "db_xrefs": [ "COG:COG1670", "COG:JO", "GO:0016747", "SO:0001217", "UniRef:UniRef50_P75087", "UniRef:UniRef90_P75087" ], "nt": "ATGGATAAAAACACTATTCTTGAGTTTTGCTTTTTTGGTGATCGTTACCAACTAATGGCACTGAAAAACCCCAATAAGTTGGTCAAAAATAAACAGGTCAAAGCTGCTTTTAATGTTTTCATGAAGCACCGGACCACCTTAAGTGCTACTAACTATATGGCGCAAATTGTGGAAAACTTTGAGCAGTTCTTGGAATACATCCACGACAAACTGCAACCCGGTAAACTGTTAGCGCTCTTATTTAAAAACAAGCAGTTTTTGGGTTGGTTTTGTGTCGGGCAATGGGGCAACATGCCGGTATTATCGTTTTGGATTGATCCCAGTCAACAACAAAGTGGTGTCGCTTTAGAAACAATTAGTTTCTTTCTTAAGTACTACCAGCGTACGTTGCAACCGCGGGAATTAGTGTGA", "aa": "MDKNTILEFCFFGDRYQLMALKNPNKLVKNKQVKAAFNVFMKHRTTLSATNYMAQIVENFEQFLEYIHDKLQPGKLLALLFKNKQFLGWFCVGQWGNMPVLSFWIDPSQQQSGVALETISFFLKYYQRTLQPRELV", "aa_hexdigest": "b1a406132be46032d970c6555964dee1", "start_type": "ATG", "rbs_motif": null, "genes": [ "rimL" ], "truncated": "5-prime", "pseudogene": { "start": 32236, "stop": 32949, "observations": { "mutation": [ 32262, 32364, 32376, 32541 ], "stop": [ 32262, 32364, 32376, 32541 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75087", "query_cov": 1.0, "subject_cov": 0.49635036496350365, "identity": 1.0, "score": 283.0, "evalue": 9.72e-95, "gene_start": 1, "gene_end": 136, "reference_start": 1, "reference_end": 136, "reference_sequence": "MDKNTILEFCFFGDRYQLMALKNPNKLVKNKQVKAAFNVFMKHRTTLSATNYMAQIVENFEQFLEYIHDKLQPGKLLALLFKNKQFLGWFCVGQWGNMPVLSFWIDPSQQQSGVALETISFFLKYYQRTLQPRELVWSFYESNQRSRHLANKLGFPSLFYFKKAEPNAPKVYFGTKDFKGESVIETKPYLGWGSGWKVNQMLKPTKNLTVNLINIGFDAYADSQKALLAWFKTHKLTKGQVEVFFNYELCCLCISYNHKQSWVVYSFQQLLAKR" }, "paralog": false, "identity": 0.9789029535864979, "score": 469.0, "evalue": 1.09e-170, "description": "Internal stop codon at 32262, 32364, 32376, 32541. Nonsense mutation around 32262, 32364, 32376, 32541.", "psc": { "uniref90_id": "UniRef90_P75087", "gene": "RimL", "product": "Uncharacterized protein MPN_027", "uniref50_id": "UniRef50_P75087", "cog_id": "COG1670", "cog_category": "JO", "go_ids": [ "GO:0016747" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75087", "UniRef:UniRef50_P75087", "COG:COG1670", "COG:JO", "GO:0016747" ] }, "pscc": { "uniref50_id": "UniRef50_P75087", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75087" ], "product": "Uncharacterized protein MPN_027" } }, "id": "GIHJHMOALN_95", "locus": "GCA009939745_00055" }, { "type": "cds", "sequence": "contig_1", "start": 32982, "stop": 33203, "strand": "+", "frame": 3, "gene": null, "product": "glycosyltransferase family 2 protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED93A4E" ], "nt": "GTGCAATTCAAGTATTTATTCACCGTGATTATTCCCACTTACAATTGTGGTCAATACATCCCAAAAGCATTAGATTCCTTGCTTTTACAAGGTGAATATTTTACTAAGACCCAAGTTTTAATCGTCAATGATGGTTCGACTGATAATACCAAGCAGATCGTAGAACCCTATACCCAACAATACAGTAACATTGAGTACTTGGAAAAACCCAATGGCAATTGA", "aa": "MQFKYLFTVIIPTYNCGQYIPKALDSLLLQGEYFTKTQVLIVNDGSTDNTKQIVEPYTQQYSNIEYLEKPNGN", "aa_hexdigest": "e4c2ed2ab68fc1a238982e4aaf22c83d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED93A4E", "query_cov": 1.0, "subject_cov": 0.8588235294117647, "identity": 0.767, "score": 113.0, "evalue": 1.71e-31, "valid": false, "product": "glycosyltransferase family 2 protein", "uniref50_id": "UniRef50_UPI001ED93A4E" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED93A4E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED93A4E" ], "product": "glycosyltransferase family 2 protein" }, "genes": [], "id": "GIHJHMOALN_96", "locus": "GCA009939745_00056" }, { "type": "cds", "sequence": "contig_1", "start": 33210, "stop": 33635, "strand": "+", "frame": 3, "gene": "wcaA", "product": "putative glycosyltransferase MG025-like protein", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.-.-", "GO:0016757", "SO:0001217", "UniRef:UniRef50_P75086", "UniRef:UniRef90_P75086" ], "nt": "GTGGTCAACTTTGTCAAACAAAACCAGTTAGCTAAGGGTCAGTACATTACTGTATTAGACAGCGATGATTATTTTCTAGCTAACGCTTTTCAACGCGTAGCTGCTCACTTTGGCCATGACATGATTGTGAGCGCCTTTTACTGTTACATTAGCCCTAAAAGAAGGCGTTTTCTCAAACCATACTTTGGTAAAACGGGGGTAATTGAACAAAAGACTAAACTACGCACACCCCATTCCCAACCCTTGGCTAAGTTCTACCGCCACGAGATCTTTAACTTGCTAGATCCTTTAAAAGAAAAGCTGTTTTACCAGGACTGTCTGTTGTACCATAATGCCATCAATAAGGTGCAAAGTGTCTTTTACATTTGTGAACCCTTAGCAGTGTGGTATGCCACTAGACCAGGTAATTCCACCACTATGCCCTGA", "aa": "MVNFVKQNQLAKGQYITVLDSDDYFLANAFQRVAAHFGHDMIVSAFYCYISPKRRRFLKPYFGKTGVIEQKTKLRTPHSQPLAKFYRHEIFNLLDPLKEKLFYQDCLLYHNAINKVQSVFYICEPLAVWYATRPGNSTTMP", "aa_hexdigest": "2c9cbb74b0c22c6b4fa1505e9225ee48", "start_type": "GTG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "both", "pseudogene": { "start": 32982, "stop": 33881, "observations": { "mutation": [ 33201, 33633, 33798 ], "stop": [ 33201, 33633, 33798 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75086", "query_cov": 1.0, "subject_cov": 0.47157190635451507, "identity": 0.986, "score": 292.0, "evalue": 6.12e-98, "gene_start": 1, "gene_end": 141, "reference_start": 77, "reference_end": 217, "reference_sequence": "MQFKYLFTVIIPTYNCGQYIPKALDSLLLQGEYFTKTQVLIVNDGSTDNTKQIVEPYTQQYSNIEYLEKPNGNWGSVVNFVKQNQLAKGQYITVLDSDDYFLANAFQRVAAHFGHDMIVSAFYCYISPKRRRFLKPYFGKTGVIEQKTKLRTPHSQPLAKFYRHEIFHLLDPLKEKLFYQDCLLYHNAINKVQSVFYICEPLAVWYATRPGNSTTMPWNNADKFQAWCDLLKQMNLYGAGIVIYIYTMLPGFLKELKRQQLVLDLAKKPAYTWLPQPLAFLFGGLMALRTRKYIRYPKN" }, "paralog": false, "identity": 0.9832775919732442, "score": 602.0, "evalue": 9.29e-223, "description": "Internal stop codon at 33201, 33633, 33798. Nonsense mutation around 33201, 33633, 33798.", "psc": { "uniref90_id": "UniRef90_P75086", "gene": "WcaA", "product": "Uncharacterized glycosyltransferase MG025 homolog", "ec_ids": [ "2.4.-.-" ], "uniref50_id": "UniRef50_P75086", "cog_id": "COG0463", "cog_category": "M", "go_ids": [ "GO:0016757" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75086", "EC:2.4.-.-", "UniRef:UniRef50_P75086", "COG:COG0463", "COG:M", "GO:0016757" ] }, "pscc": { "uniref50_id": "UniRef50_P75086", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75086" ], "product": "Uncharacterized glycosyltransferase MG025 homolog" } }, "id": "GIHJHMOALN_97", "locus": "GCA009939745_00057" }, { "type": "cds", "sequence": "contig_1", "start": 33681, "stop": 33800, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTATACGGCGCGGGTATAGTGATTTACATTTACACTATGTTACCTGGTTTTTTAAAAGAATTAAAACGGCAACAACTAGTGTTAGATTTAGCTAAAAAACCCGCTTATACCTGA", "aa": "MNLYGAGIVIYIYTMLPGFLKELKRQQLVLDLAKKPAYT", "aa_hexdigest": "3898c01e7da5abe6be54c273b7f2bc05", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4474.375000000002, "isoelectric_point": 9.522770118713382 }, "id": "GIHJHMOALN_98", "locus": "GCA009939745_00058" }, { "type": "cds", "sequence": "contig_1", "start": 33902, "stop": 34474, "strand": "+", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043", "KEGG:K02356", "RefSeq:WP_010874386.1", "SO:0001217", "UniParc:UPI0000129D0D", "UniRef:UniRef100_P75085", "UniRef:UniRef50_P75085", "UniRef:UniRef90_P75085" ], "nt": "ATGGCAGACATGATTGAAGCGAAAAGTTTACGCAGTGGACAAACGATTTTTGGTCCCAATAAAGAGATTCTGTTGGTGTTAGAAAACACCTTTAATAAGACCGCAATGCGCCAGGGAATTGTTAAAACCAAGGTCAAAAACTTACGCACTGGGGCCATTGTTTGGATTGAGTTTACCGGAGACAAGTTAGAACAAGTCATTATTGACAAGAAGAAAATGACCTTCTTGTACAAGGATGGTGCTAACTATGTTTTTATGGACCAACAAGACTACAGTCAAATTGAGATCCCCGAAAAGCAGTTAGAGTGGGAAAAGAACTTTATCACTGAAGACAGTGAAGTTACTATTATTAGCTACCAAAGTGAAATTTTGGGCGTTAACCTACCCGAATTAGTGCCGATTGAAGTGGAATTTGCCGAAGAAGCAGTGCAAGGTAATACCGCTAACATGGCGCGTAAACGGGCTCGGTTAGTTTCGGGTTACGAATTAGATGTGCCCCAGTTCATTCGTACTGGTGATAAGATTGTGATTTCCACCATTGACGGGAGCTATCGTGAACGGTACAACAAGTAA", "aa": "MADMIEAKSLRSGQTIFGPNKEILLVLENTFNKTAMRQGIVKTKVKNLRTGAIVWIEFTGDKLEQVIIDKKKMTFLYKDGANYVFMDQQDYSQIEIPEKQLEWEKNFITEDSEVTIISYQSEILGVNLPELVPIEVEFAEEAVQGNTANMARKRARLVSGYELDVPQFIRTGDKIVISTIDGSYRERYNK", "aa_hexdigest": "ed93e08bd627e0e94ccd9d96fdcbd1c0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129D0D", "ncbi_nrp_id": "WP_010874386.1", "uniref100_id": "UniRef100_P75085", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129D0D", "RefSeq:WP_010874386.1", "UniRef:UniRef100_P75085" ] }, "ips": { "uniref100_id": "UniRef100_P75085", "uniref90_id": "UniRef90_P75085" }, "psc": { "uniref90_id": "UniRef90_P75085", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_P75085", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043" ] }, "pscc": { "uniref50_id": "UniRef50_P75085", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75085" ], "product": "Elongation factor P" }, "genes": [ "efp" ], "id": "GIHJHMOALN_99", "locus": "GCA009939745_00059" }, { "type": "cds", "sequence": "contig_1", "start": 34455, "stop": 34898, "strand": "+", "frame": 3, "gene": "nusB", "product": "putative MG027-like protein", "db_xrefs": [ "COG:COG0781", "COG:K", "RefSeq:WP_014574845.1", "SO:0001217", "UniParc:UPI0001DD6462", "UniRef:UniRef100_A0AAN4XFP4", "UniRef:UniRef50_P47273", "UniRef:UniRef90_P75084" ], "nt": "GTGAACGGTACAACAAGTAAATTAACTAGAACCCAAAGACGGATTGCAATTGTGGAGTTTATCTTCGCCACGCTGTTCTTTTTACCGAAAACTGCTGACCAAATCCAAGCGGCCTTTTTGGACTATGATGTGCCAGAACGTCCGCTCAACGATTGGCAAAAAGAGATTGTCAAAGTCTTTAGTGAACGTTGTGTGGAATTCATTGAACTAATTGAAAACCAACAACAACGCAACCAAGCAGAAGTACAAAGCAAGTACAACAAGGTTTCCGGTAAAAAAGTCGACTTATTGACTAAGGCCGTGATTCTCTGTGCTTTGAGCGAACAACACGCGCAAGCGACTGATAAACCATTATTAATTAGTGAAGCCTTACTGATTATGGACCACTATTCCCAGGTACCCGAAAAGAAGCAAACCCATGCTTTATTGGATAAACTCTTGTAA", "aa": "MNGTTSKLTRTQRRIAIVEFIFATLFFLPKTADQIQAAFLDYDVPERPLNDWQKEIVKVFSERCVEFIELIENQQQRNQAEVQSKYNKVSGKKVDLLTKAVILCALSEQHAQATDKPLLISEALLIMDHYSQVPEKKQTHALLDKLL", "aa_hexdigest": "e029943092bf8c0c09f57c14baf6f38b", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6462", "ncbi_nrp_id": "WP_014574845.1", "uniref100_id": "UniRef100_A0AAN4XFP4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6462", "RefSeq:WP_014574845.1", "UniRef:UniRef100_A0AAN4XFP4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAN4XFP4", "uniref90_id": "UniRef90_P75084" }, "psc": { "uniref90_id": "UniRef90_P75084", "gene": "NusB", "product": "Uncharacterized protein MG027 homolog", "uniref50_id": "UniRef50_P47273", "cog_id": "COG0781", "cog_category": "K" }, "pscc": { "uniref50_id": "UniRef50_P47273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47273" ], "product": "Uncharacterized protein MG027" }, "genes": [ "nusB" ], "id": "GIHJHMOALN_100", "locus": "GCA009939745_00060" }, { "type": "cds", "sequence": "contig_1", "start": 34928, "stop": 35137, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGTAGCAAACCTAGCCTTAGTGTTGGGTTTTATGTTGAACATTGTGGTGGCGCGGTATACCTTAACTGGTGCTACGCCCCAAGCACGTTTTTTATTCCTCACACCCTTTTTAGGCATAGTAGCAGCGAGTATCTTCTACTTCTTTGATGTGAAGTGGTTCTTAGCGGATTATCCCTACAAGAAGTTCCATTTTCAAAAGAAGTGA", "aa": "MFVANLALVLGFMLNIVVARYTLTGATPQARFLFLTPFLGIVAASIFYFFDVKWFLADYPYKKFHFQKK", "aa_hexdigest": "7c4ba972575b761923c09e97ff050c0d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8018.525199999996, "isoelectric_point": 9.90029697418213 }, "id": "GIHJHMOALN_101", "locus": "GCA009939745_00061" }, { "type": "cds", "sequence": "contig_1", "start": 35294, "stop": 35509, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTGTTAGTATCTTTTTGAGCTTAATTAGTATTGGTTTGAGCAAAACAGCCCACTTTAAAATTGATGTGGAAATGCTCAAAGCGAAAAAAGGCGAACCCACCGCCGCTGATAAAACCGACAGCCGGCCGGTAGTTGTTGATTTAGACCAAACTAAATCAAAAAAAGATGGGGATAACCCACCCCAAGCTTCGGGTGATATGACTAGTCTTTAG", "aa": "MGVSIFLSLISIGLSKTAHFKIDVEMLKAKKGEPTAADKTDSRPVVVDLDQTKSKKDGDNPPQASGDMTSL", "aa_hexdigest": "b66b2a8944333d45e3b66390bb9894b4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7560.590500000006, "isoelectric_point": 6.538212013244629 }, "id": "GIHJHMOALN_102", "locus": "GCA009939745_00062" }, { "type": "cds", "sequence": "contig_1", "start": 35733, "stop": 35846, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGCTACCAAGGTATTAGACCATGACCAACAGTTACTCGACACTATCCGTTACTTTAAGGATCATGACAAGTATGTCTTTGCCATTTGTGATACCCCCAACGTGTTGTAA", "aa": "MGATKVLDHDQQLLDTIRYFKDHDKYVFAICDTPNVL", "aa_hexdigest": "f05ffe5d3d2b180244c240d37605fb3c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4324.8874000000005, "isoelectric_point": 5.280194664001464 }, "id": "GIHJHMOALN_103", "locus": "GCA009939745_00063" }, { "type": "cds", "sequence": "contig_1", "start": 35893, "stop": 36060, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386D08", "UniRef:UniRef100_A0AB33HPV8", "UniRef:UniRef50_A0AB33HPV8", "UniRef:UniRef90_A0AB33HPV8" ], "nt": "ATGAAAAAACTCTTAGTCATTGTTTACCCTGACATGAATGATGTGGAATACACCAACACAATGGTGGTATTTGGTTTTGTTAAAGAATTACAAACCGTGATTTATCACCCTAACTTAAGTACAGTTAAGGGCAGTAATGGGGTAACATTTAGTAAACCAGATTACTAG", "aa": "MKKLLVIVYPDMNDVEYTNTMVVFGFVKELQTVIYHPNLSTVKGSNGVTFSKPDY", "aa_hexdigest": "e5096da9bb6ddd439ae0a82129638ee5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386D08", "uniref100_id": "UniRef100_A0AB33HPV8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386D08", "UniRef:UniRef100_A0AB33HPV8" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HPV8", "uniref90_id": "UniRef90_A0AB33HPV8" }, "psc": { "uniref90_id": "UniRef90_A0AB33HPV8", "uniref50_id": "UniRef50_A0AB33HPV8" }, "pscc": { "uniref50_id": "UniRef50_A0AB33HPV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HPV8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6272.223600000003, "isoelectric_point": 6.520194053649903 }, "id": "GIHJHMOALN_104", "locus": "GCA009939745_00064" }, { "type": "cds", "sequence": "contig_1", "start": 36064, "stop": 36684, "strand": "-", "frame": 1, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0044206", "RefSeq:WP_010874390.1", "SO:0001217", "UniParc:UPI0000137D2C", "UniRef:UniRef100_P75081", "UniRef:UniRef50_P47276", "UniRef:UniRef90_P75081" ], "nt": "GTGATCAAAAAAGTGCACCACGCTTTGATCGAAAACGAATTGACAAAGCTACGTGATAAATCTTCTTCCTCCTCGCAATTTCGGATGGCGTTAAGCCAAATCACTTCACTACTCTTTTTTGAAGTAACTAAGGAATCACCCTTAGAAGAAATTGTTGTAAAAACCCCGTTTACCAAAACGAAGGGTTATAAGCTCAAAAACGAAATTGTTCTAGTCCCCATAATGCGCGCTGGCCTGGGTATGGTTGACCCAATTGTGCGCTATTCGGAAAAGATTCGTGTGGGTCACTTAGGGATCTACCGTGAACATGAGGGCACTAATGTAATTTCTTACTATAAGAAAATGCCCGAAAACATTGGCAACTCCCACGTGATTATCTTAGACCCAATGCTAGCTACTGGTACTACCCTAGCCACAGCCATTAGTTCCATTAAAAAGAACCACCCGATTAAGATTAGTGTGGTGGCCATAGTAGCAGCACCAGAGGGCATTAAAGCCGTGGAAGCAGCGCACCCTGATGTGGACATTTACCTAGCAGCCATTGATGAAAAATTAAATAAGGATAATTACATTATTCCCGGGCTTGGTGATGCTGGGGATCGCTTATTTGGTACTAAATAA", "aa": "MIKKVHHALIENELTKLRDKSSSSSQFRMALSQITSLLFFEVTKESPLEEIVVKTPFTKTKGYKLKNEIVLVPIMRAGLGMVDPIVRYSEKIRVGHLGIYREHEGTNVISYYKKMPENIGNSHVIILDPMLATGTTLATAISSIKKNHPIKISVVAIVAAPEGIKAVEAAHPDVDIYLAAIDEKLNKDNYIIPGLGDAGDRLFGTK", "aa_hexdigest": "92a11133979695b8a841da5a0124591c", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137D2C", "ncbi_nrp_id": "WP_010874390.1", "uniref100_id": "UniRef100_P75081", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137D2C", "RefSeq:WP_010874390.1", "UniRef:UniRef100_P75081" ] }, "ips": { "uniref100_id": "UniRef100_P75081", "uniref90_id": "UniRef90_P75081" }, "psc": { "uniref90_id": "UniRef90_P75081", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_P47276", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_P47276", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47276" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "GIHJHMOALN_105", "locus": "GCA009939745_00065" }, { "type": "cds", "sequence": "contig_1", "start": 36724, "stop": 37185, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12836.13", "PFAM:PF14579.12" ], "nt": "TTGAGCTACTATGCTTGTTTGTTGAGCTTTAAACTCAAGGAACATGATGTCAGTGGTTTTAAATCAGGTGTGAGCTTTGTCAAGCAAAAACTAGAGGAGCTCAACACACTGTACCGCATTAAACGGATTAAACCCAAAGAAGCAGAACTGTTAACCAGTTACGAGGTTTATTTAGAGATGATGGCCCGCGGCATTAAACTAGAACAAATCTCTTTAACCCACTCCCACGCCACTCGTTTTGTCGAGCACAACGGCATTCTAATTGCCCCTTTTATTACGATTCCGGGGATGGGGGAAGCTGTGGCCAATTCGATCATAGAAGCGCGTAACGAAAAGCCCTTTAGTTCGCTCGATGACTTTAAAAAACGCACTAAAATTACCAAAAAACACATTGAAGCGTTTACCCAAATGCAACTACTCGATGAGTTTAGGGAGCAAGACAACCAGAAAAAACTTTTTTAG", "aa": "MSYYACLLSFKLKEHDVSGFKSGVSFVKQKLEELNTLYRIKRIKPKEAELLTSYEVYLEMMARGIKLEQISLTHSHATRFVEHNGILIAPFITIPGMGEAVANSIIEARNEKPFSSLDDFKKRTKITKKHIEAFTQMQLLDEFREQDNQKKLF", "aa_hexdigest": "7b4e8271f14a86fab705caf0f89ce296", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12836.13", "name": "HHH_3", "length": 42, "aa_cov": 0.2679738562091503, "hmm_cov": 1.0, "evalue": 2.974049465608747e-09, "score": 32.41563034057617, "start": 93, "stop": 133 }, { "id": "PF14579.12", "name": "HHH_6", "length": 87, "aa_cov": 0.5424836601307189, "hmm_cov": 0.9885057471264368, "evalue": 6.839556435112091e-16, "score": 53.801841735839844, "start": 64, "stop": 146 } ], "seq_stats": { "molecular_weight": 17774.452899999986, "isoelectric_point": 9.097536659240724 }, "id": "GIHJHMOALN_106", "locus": "GCA009939745_00066" }, { "type": "cds", "sequence": "contig_1", "start": 37216, "stop": 37749, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGTTAAAGCACCTGGCCGATCTTACCCATGTCAATCCACAAAACATTCCCCGGTTTGACAAGAAGCTCACCGAAATGTTTTGGTCGGTCAATCCCTTAAAACTAAAACCGCACTACTTAGATGAACCCACGGGAGCGATTGGGATTCCCGAGTTTGGTACTAAGTTCGTTCGCAAGATCTTGGAACAAACCAAACCAAAGGGCTTTGGTGACCTCATTCGCGTCTCGGGTCTAAGTCACGGGAAGAACGTGTGGGCGGACAATGCGCAGAAAATCTTGAAGGACCAAAACTTAAGCTTAAAGGATGTAATTGCCTGTCGCGATGACATTATGCTCTACCTCATTCATAAGGGGATGCAGGCCAAAGATGCTTTTGAGATCATGGAAAAGGTGCGTAAGGGAATTGCTCTCAATGCCAAGGAAGTGCAATTAATGCAGAGCAATGGGGTCGAACAACACTGGATTAATTCGTGTTTAAAGATTAGCTACCTGTTCCCGAAAGCCCACGCCGCGGCTTATGTTTTAATGGCCTGA", "aa": "MLKHLADLTHVNPQNIPRFDKKLTEMFWSVNPLKLKPHYLDEPTGAIGIPEFGTKFVRKILEQTKPKGFGDLIRVSGLSHGKNVWADNAQKILKDQNLSLKDVIACRDDIMLYLIHKGMQAKDAFEIMEKVRKGIALNAKEVQLMQSNGVEQHWINSCLKISYLFPKAHAAAYVLMA", "aa_hexdigest": "8fa18bb424c0300ae655adab1ba74e3c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 162, "aa_cov": 0.7853107344632768, "hmm_cov": 0.9135802469135802, "evalue": 1.178658538039685e-25, "score": 85.28016662597656, "start": 2, "stop": 140 } ], "seq_stats": { "molecular_weight": 20107.401399999995, "isoelectric_point": 9.373783683776853 }, "id": "GIHJHMOALN_107", "locus": "GCA009939745_00067" }, { "type": "cds", "sequence": "contig_1", "start": 37828, "stop": 38754, "strand": "-", "frame": 1, "gene": null, "product": "DNA polymerase III alpha subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W4V224" ], "nt": "GTGTTTGTGCGTACCACAGGGGAAATGCTCAATGAAATGAGTTTTTTAGGCGAACAATTAGCTTACGAGTTAGTGGTCAAAAACACCAATAAGTTAGCGAAACAACTCACCGCTGATGACCTGGTACCAGTGCAAACCAAACTCCAACCACCAGTAATTGAAGGTTCTAACGAAAACCTGGCAGCCAAAACCTGGAGTCAAGCCAAGGCCATTTACGGTGATCCCTTACCTAAGTTAATAGAACAACGGATCCAGGAAGAGTTAAAAGCGATCATTGACAATGGCTTTGGCATTATTTACTGGATTTCCCATTTACTAGTCAAACAATCGGTTCAAGATGGTTACTTTGTTGGACCGCGGGGTTCAATTGGTTCTTCGTTGGTAGCTAACCTGATTGGTATCTCGGAAATTAATCCCTTAGTGCCGCATTATCTCTGTGAGAGCTGTCAGTACTTTGAGGTCAACGAAGAGGTCGATGATGGGTATGACCTCATGGTAAGGGATTGTCCAAAGTGTGGTGCCAAAGCAGCCTTTAAGGGTGATGGTCATAACATTCCCTTTGCTACCTTTATGGGTTTTGCTGGGGACAAGATTCCCGATATTGACTTAAACTTTTCCAGTGAGTACCAAGCCAAAGCCCATGCTTATGTGAGGGAACTGTTTGGTGAACAGTACACCTTCCGTGCTGGCACAATTGCGACAGTGGCCGAAAAAACGGCTTACGGGTATGCGCGCAACTACTTTGAAATTATTAAACAAACCGAATTGGCCACCGCTCCCGAAATTGAACGCTTTAAGCAAAAGTTAGTCGGGATTAAACGCACGACCGGCCAACACCCCGGGGGGATTATGATCTTCCCCAACCACAAATCGGTATATGAGTTTACCCCGTGTGGTTATCCAGCTGATGATACTTCCAGTGATTGA", "aa": "MFVRTTGEMLNEMSFLGEQLAYELVVKNTNKLAKQLTADDLVPVQTKLQPPVIEGSNENLAAKTWSQAKAIYGDPLPKLIEQRIQEELKAIIDNGFGIIYWISHLLVKQSVQDGYFVGPRGSIGSSLVANLIGISEINPLVPHYLCESCQYFEVNEEVDDGYDLMVRDCPKCGAKAAFKGDGHNIPFATFMGFAGDKIPDIDLNFSSEYQAKAHAYVRELFGEQYTFRAGTIATVAEKTAYGYARNYFEIIKQTELATAPEIERFKQKLVGIKRTTGQHPGGIMIFPNHKSVYEFTPCGYPADDTSSD", "aa_hexdigest": "5fad5e154f3144b580c139003257883a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_W4V224", "query_cov": 0.8798701298701299, "subject_cov": 0.8187311178247734, "identity": 0.513, "score": 292.0, "evalue": 1.1e-94, "valid": false, "product": "DNA polymerase III alpha subunit", "uniref50_id": "UniRef50_W4V224" }, "pscc": { "uniref50_id": "UniRef50_W4V224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W4V224" ], "product": "DNA polymerase III alpha subunit" }, "genes": [], "id": "GIHJHMOALN_108", "locus": "GCA009939745_00068" }, { "type": "cds", "sequence": "contig_1", "start": 38800, "stop": 39591, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGGTAGCCCATAACGGTTTGAGTTTTGACTTGCCCTTTTTACAAACCCAGTTTGAAAAATACAACATTGCGCCACTAACCAATCCCTTAATTGATACCTTAGCCTTATCCTGGGCCTTAAATCCCGGGTTTGCCTCCCATACGTTGAGCAATATTTGTGCCAAGCTCAAGTTTGACTTTGATGATGAACGATTGCACCGAGCGGATTATGATACCCAGGCCTTAAAGAAGGTCTTTGACTACTTCAAGGAACAGGTCGAACTCATGGGGATTACTAATCTCGAGCAGTTAGACCAAGAGTTAAACCAGCAGTGCCACTTTGAACTGCTCAAGCGCACTTTTACCAATACCGGCATTATCTACATTAAGAGCCAAAGTGGGTTTGCTAAACTGTATGAACTGTTATCAATTGCGCTCACCGATAACAACGCTACTCGACCCCTAGTGTTAACTTCAACACTCCAAAAGTTTGCTAAATCCTTTGTCATTACTGATAACCCGGTGCAAGGTGATATCTTTAAAGCGGCATTGACTAAACCGCTTAAAGAATTAGAAGCGGCCATTAAAAGAGTTGACTTTGTTTTAATTGCCCCACCTGATGCTTATGCTGGTTACACCATCAGGGAAGGTTTGAAAAAAGAAGCCATTCCTAACGCAATTAAGTTAGTGGTGGACACGGCCCAAAGACTCAATAAGTTAGTGGCAGTTGCCTCGGATGCTTACTTTATTCACCCCTGGGAGAATGAGTACTATAAGGCCATAGTTTGTGCCAAGGGTCTGGGTGGGCGTTGA", "aa": "MVAHNGLSFDLPFLQTQFEKYNIAPLTNPLIDTLALSWALNPGFASHTLSNICAKLKFDFDDERLHRADYDTQALKKVFDYFKEQVELMGITNLEQLDQELNQQCHFELLKRTFTNTGIIYIKSQSGFAKLYELLSIALTDNNATRPLVLTSTLQKFAKSFVITDNPVQGDIFKAALTKPLKELEAAIKRVDFVLIAPPDAYAGYTIREGLKKEAIPNAIKLVVDTAQRLNKLVAVASDAYFIHPWENEYYKAIVCAKGLGGR", "aa_hexdigest": "1dcf9f2dca7f7c2681c23bba5e70d085", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 65, "aa_cov": 0.24714828897338403, "hmm_cov": 0.9538461538461539, "evalue": 1.046245825694221e-06, "score": 24.250858306884766, "start": 1, "stop": 65 }, { "id": "PF00929.31", "name": "RNase_T", "length": 81, "aa_cov": 0.30038022813688214, "hmm_cov": 0.9629629629629629, "evalue": 2.315751072791111e-15, "score": 52.992740631103516, "start": 1, "stop": 79 } ], "seq_stats": { "molecular_weight": 29623.76490000001, "isoelectric_point": 6.528208351135253 }, "id": "GIHJHMOALN_109", "locus": "GCA009939745_00069" }, { "type": "cds", "sequence": "contig_1", "start": 39607, "stop": 40068, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00929.31" ], "nt": "ATGAGTGCCTTTGATGGCATTAACAGTGTCCAGGAATACGCCCAGTTTGCCAAAGAACGCGATTGGAAGACAATTGCTGTCACTGACAAGGACAACATTCACATTTATCCCACTTTATATGAAGTGGCCAAAAAGTACGGCTTGAAGGCCATTTATGGTTTGGAATGTAACCTCATTGATGATCACATTAAGATAGTTAGCAATCCTGATAAAACCAAACTTAAGGATGCGACCTTTGTCATCTTCGACATTGAAACAACTGGGTTACACGGTCGGTATGACTCGGTGATTGAGTTTGCGGGAATTAAGGTCAAACATAACCGTGAAGTGGAACGAATGCAGTTCTTTCTCAAGATTGATGGACCCTTACCTGCAGCAGTTACCGAAATTACCAAGATTACCCAAGCACAACTAGAAGATGGGATGGAACAGCAAGCGGGGTTGGAAAAACTGCGCGCTTGA", "aa": "MSAFDGINSVQEYAQFAKERDWKTIAVTDKDNIHIYPTLYEVAKKYGLKAIYGLECNLIDDHIKIVSNPDKTKLKDATFVIFDIETTGLHGRYDSVIEFAGIKVKHNREVERMQFFLKIDGPLPAAVTEITKITQAQLEDGMEQQAGLEKLRA", "aa_hexdigest": "d2a265eba449a44e251e5e808abb06eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00929.31", "name": "RNase_T", "length": 77, "aa_cov": 0.47058823529411764, "hmm_cov": 1.0, "evalue": 5.198103615833917e-17, "score": 58.354957580566406, "start": 80, "stop": 151 } ], "seq_stats": { "molecular_weight": 17379.67629999998, "isoelectric_point": 5.581043434143066 }, "id": "GIHJHMOALN_110", "locus": "GCA009939745_00070" }, { "type": "cds", "sequence": "contig_1", "start": 40219, "stop": 40827, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAGGGGCAGAACTGAGGATTACCTTCCAAGAAGATGCCACCTTCTTTAAGGACGGGTCCACTAAGTCCAGTGTCACCTTAGCCATTATCAAAGACTACTTCAAAAGCTTCTTTGGTAAGGACAAGAAGTACCGCATCCTGTTAGAGCAAGAGTTAACCCATCCTAACTTCTTGTCCTACAGTAACCATGAACTGAAAGCTAACTGCCAGTCCCAAGAATTAGACCAATGGTTAATTGAACAACGCCAAGCCTTCATTAAGTGGATGCACCAAGCTGGGTTTACCCACTTTGGTTTTGTCTCGTTGTTTAATCCACCAGCGGAAAAACAACTAAAAGTGAAATCAATGAAGGTATCCAAGTACGACAAGCAGTTTGAAACCGAGGTCTTTAGTACGGAATTTGTCCCCATTCACAAGATTAACCAACAAATGGATGAGATCAAACTAATGGGGCAAATCTTTGAGTTAAAGGACTTTCCTGGGTACAACAACCTCCGTAATACCCTTAATATTTATGTCACTGATTTCCAACTAGGTGGTTCGTTAATTTTGAAGTGGTTTTACAAGGATCCCAAAACAATTGAGGGCATTAAGATTGGTACCTGA", "aa": "MEGAELRITFQEDATFFKDGSTKSSVTLAIIKDYFKSFFGKDKKYRILLEQELTHPNFLSYSNHELKANCQSQELDQWLIEQRQAFIKWMHQAGFTHFGFVSLFNPPAEKQLKVKSMKVSKYDKQFETEVFSTEFVPIHKINQQMDEIKLMGQIFELKDFPGYNNLRNTLNIYVTDFQLGGSLILKWFYKDPKTIEGIKIGT", "aa_hexdigest": "3282a0396ec0ece36a1a23b3d7268cd1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23706.92160000001, "isoelectric_point": 7.778513526916504 }, "id": "GIHJHMOALN_111", "locus": "GCA009939745_00071" }, { "type": "cds", "sequence": "contig_1", "start": 40855, "stop": 41055, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTCGACACCGAAACCGAAAAGGGTAAACGCATTTTAGCATTATCACAGTTTTTAGTTAAGAAAAATATTCTCGATCACAACGAATTGCACCAACTCAACAACCGGATTGAACTGGTTTACCTCGAGAATGATCGCGAAAACGCCCTGTTTCTCGTGGCGCTCACTTTAAAAAAACCGTTAACAATTGACATTTGA", "aa": "MVFDTETEKGKRILALSQFLVKKNILDHNELHQLNNRIELVYLENDRENALFLVALTLKKPLTIDI", "aa_hexdigest": "0ae06d94c8d77533384386b26ca26e00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7733.936899999996, "isoelectric_point": 6.054341697692871 }, "id": "GIHJHMOALN_112", "locus": "GCA009939745_00072" }, { "type": "cds", "sequence": "contig_1", "start": 41333, "stop": 42484, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_035", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078", "UniRef:UniRef90_P75079" ], "nt": "ATGAAAGCGTTCCGAAAATTGTTGCTGGGTCTCGCCTTACCCACAACCATCGGTCCCTTATTAGGCTTATTATTAACTAATACTGATACTGTCAAAAATGAAAGCTTAACTACTGTGCGCCACAGGTCCAGCATTAACCAGGACTTTGATGGCATTTTTCATACCGTTAAGTTATTAGAACCAATCCAGCAAAGTAATGCTGATCCCGCCGCTAGTTTTGCGCTCTTTCAGCAAAAGTTTCCCAACCTAAAACGTGTAGCCAACTCCACCTTAAATACCTTTGATGTCTACAACCTTTTGAGTGGTTGGAAGAGTAGCTTAACAGCGTACCTCAACCAGGTGTTGGCACTCCAACAACGGATTAAAGCAGCTGATCAAATTTTCCCTAACCAAAAGGAAACTTTACCCAAGGATGAGAATCCCAACATCTTTGAAGTGTTAGGTGCATATGGTGGTAAGGGTTTCTTTCCCACGCTAGGATCCAACGGGTTACACCTGCCACCACAGCTGTTCCAGTTCTTCCGTGACTTCCAACTAAGTAGCTTTACCATTAAAGACTTTGAGGTAGCGTTAGTGAGTGAACCGGACATAGTGCAACATGACAAAGTGCGCTATGCCTTTCAGGTGCAATTTAACTTAGTGTTACAATTACAAATTAACCGTAACCCAGTGTTATTTGACTTTAACCTACAGTTAAAGACGAATAACTTTGCCAATCAAACCAGTTTTGATGAATTGTTTAACGAAAAAACTAATCCACTTAACTGGCAGTTCTTTAGCAAGCTTAAAGTTAACCATTTAGTGTATGAAGGCAATGACATAACTCAGTTAGCTAATACCTTATTACAAAGCCAGTTTAATGTCTTACAACTTGATTTAAATAAGTCAATTTACCGCTTGAACTTAAACGCAATGGCACAGCGCTTTGAACACGACTGGGTACAACCGTTGTATGCCAAACGCACGCAGGCCAAAATTGCTTACGAAGCGGAACAAGCACGGATTGCGGCCGAGAACAAACGCAAGGAACTAGAACGGCAAAAGCTGTTAGCAGAATTGAAAGCCAAAGCTGAGCATTACCAAAAGATCAAGCAAGCACGGGAAAACATGCTAAAGGGCTTAAAGAGTATTACTGATTTTGTCAAGTTCTGA", "aa": "MKAFRKLLLGLALPTTIGPLLGLLLTNTDTVKNESLTTVRHRSSINQDFDGIFHTVKLLEPIQQSNADPAASFALFQQKFPNLKRVANSTLNTFDVYNLLSGWKSSLTAYLNQVLALQQRIKAADQIFPNQKETLPKDENPNIFEVLGAYGGKGFFPTLGSNGLHLPPQLFQFFRDFQLSSFTIKDFEVALVSEPDIVQHDKVRYAFQVQFNLVLQLQINRNPVLFDFNLQLKTNNFANQTSFDELFNEKTNPLNWQFFSKLKVNHLVYEGNDITQLANTLLQSQFNVLQLDLNKSIYRLNLNAMAQRFEHDWVQPLYAKRTQAKIAYEAEQARIAAENKRKELERQKLLAELKAKAEHYQKIKQARENMLKGLKSITDFVKF", "aa_hexdigest": "47b06f7267b664b8cc264cc9e25d3c55", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 41333, "stop": 42787, "observations": { "mutation": [ 42482, 42590, 42656 ], "stop": [ 42482, 42590, 42656 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75079", "query_cov": 1.0, "subject_cov": 0.575075075075075, "identity": 1.0, "score": 740.0, "evalue": 5.56e-265, "gene_start": 1, "gene_end": 383, "reference_start": 1, "reference_end": 383, "reference_sequence": "MKAFRKLLLGLALPTTIGPLLGLLLTNTDTVKNESLTTVRHRSSINQDFDGIFHTVKLLEPIQQSNADPAASFALFQQKFPNLKRVANSTLNTFDVYNLLSGWKSSLTAYLNQVLALQQRIKAADQIFPNQKETLPKDENPNIFEVLGAYGGKGFFPTLGSNGLHLPPQLFQFFRDFQLSSFTIKDFEVALVSEPDIVQHDKVRYAFQVQFNLVLQLQINRNPVLFDFNLQLKTNNFANQTSFDELFNEKTNPLNWQFFSKLKVNHLVYEGNDITQLANTLLQSQFNVLQLDLNKSIYRLNLNAMAQRFEHDWVQPLYAKRTQAKIAYEAEQARIAAENKRKELERQKLLAELKAKAEHYQKIKQARENMLKGLKSITDFVKFWKSPDRLLVGFNKQDDITTRAGVYKALQIAYANYPQWTFYLTLQGWKNGSELLLKRPSWTNLLSDIHFQKAFNLKNTVSEQTLGAATLPGYGYYGLRMSDWLRWALGYYSYIHMGVPQNVQTKFTGTPDNEQQVWIANEPFEWNKHYGVGPKYKDKAYRFNMEISFELEGWIAVKWWAWAFKGSIPGNWKGKLKVTHLFDGMVPVWELGPVNTHLPQYSFTDQQQLLFVPHSIQKIEAIGADKGINDLLKTQNLHNLERLSYESTNPIDLISYLLYAIQYIKV" }, "paralog": false, "identity": 0.993801652892562, "score": 952.0, "evalue": 0.0, "description": "Internal stop codon at 42482, 42590, 42656. Nonsense mutation around 42482, 42590, 42656.", "psc": { "uniref90_id": "UniRef90_P75079", "product": "Uncharacterized protein MPN_035", "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75079", "UniRef:UniRef50_P75078" ] }, "pscc": { "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078" ], "product": "Uncharacterized protein MPN_036" } }, "id": "GIHJHMOALN_113", "locus": "GCA009939745_00073" }, { "type": "cds", "sequence": "contig_1", "start": 42773, "stop": 42994, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAGTGACTGGTTGCGCTGGGCTTTGGGCTATTACTCCTATATCCATATGGGCGTACCGCAAAATGTACAGACCAAGTTTACGGGCACCCCAGATAATGAACAACAAGTCTGGATTGCTAACGAACCGTTTGAATGGAACAAACATTACGGTGTTGGTCCTAAATACAAGGATAAGGCCTACCGCTTTAACATGGAAATTAGTTTTGAACTAGAGGGTTGA", "aa": "MSDWLRWALGYYSYIHMGVPQNVQTKFTGTPDNEQQVWIANEPFEWNKHYGVGPKYKDKAYRFNMEISFELEG", "aa_hexdigest": "84fb77351fc69cd429b6d2c85d627e64", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 69, "aa_cov": 0.9452054794520548, "hmm_cov": 0.9420289855072463, "evalue": 3.8858086747876357e-19, "score": 64.58252716064453, "start": 5, "stop": 73 } ], "seq_stats": { "molecular_weight": 8738.680300000002, "isoelectric_point": 5.5347196578979485 }, "id": "GIHJHMOALN_114", "locus": "GCA009939745_00074" }, { "type": "cds", "sequence": "contig_1", "start": 43085, "stop": 43333, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGGTACCAGTGTGGGAATTGGGCCCAGTCAATACCCATTTACCCCAATACAGCTTTACCGATCAGCAACAACTGTTGTTTGTACCCCACTCCATTCAAAAGATTGAAGCGATTGGTGCGGACAAAGGTATAAACGATCTTTTAAAAACCCAAAACCTCCATAACCTAGAGCGCTTGTCATACGAATCAACTAACCCAATTGACCTCATTTCTTACCTCTTGTACGCGATCCAGTACATTAAGGTTTAG", "aa": "MVPVWELGPVNTHLPQYSFTDQQQLLFVPHSIQKIEAIGADKGINDLLKTQNLHNLERLSYESTNPIDLISYLLYAIQYIKV", "aa_hexdigest": "7edf4e6e1b6f8935ebc8151ddee24830", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 36, "aa_cov": 0.43902439024390244, "hmm_cov": 1.0, "evalue": 6.818893407173506e-12, "score": 41.1209716796875, "start": 2, "stop": 37 } ], "seq_stats": { "molecular_weight": 9438.723299999994, "isoelectric_point": 5.375115776062012 }, "id": "GIHJHMOALN_115", "locus": "GCA009939745_00075" }, { "type": "cds", "sequence": "contig_1", "start": 43505, "stop": 43816, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGAAAACACTGCACAAGTTGCTGTTGGGGCTTTCCTTACCAGCAATCCTCGGTCCGTTGTTTGGTCTACTAGTTACAAAAACTGATGCTGAATCACCAGTTGCAGCATTACATAAATCGAAATCTTTTAGCAAAAACTTAGATAGTTTCCGCGCCTTATTTGTGGGCGAGGATATTGAGGAACAACTAGCTAAGGTACAACAACAAACACCAGAAGTTAGCTTTGCTAGCTTTCAACAAAAGTTTCCTGATAAGGCCAGTTTAAGAAACGGTTTTCAACCAATTGATGTTTACAACTTTTTAAGTGGTTGA", "aa": "MKTLHKLLLGLSLPAILGPLFGLLVTKTDAESPVAALHKSKSFSKNLDSFRALFVGEDIEEQLAKVQQQTPEVSFASFQQKFPDKASLRNGFQPIDVYNFLSG", "aa_hexdigest": "c35a577a259b83e413296f3984acc7c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 40, "aa_cov": 0.3786407766990291, "hmm_cov": 1.0, "evalue": 1.584560891429048e-11, "score": 39.49834060668945, "start": 65, "stop": 103 } ], "seq_stats": { "molecular_weight": 11350.95979999999, "isoelectric_point": 8.027168083190919 }, "id": "GIHJHMOALN_116", "locus": "GCA009939745_00076" }, { "type": "cds", "sequence": "contig_1", "start": 43838, "stop": 44278, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "TTGGCTAAGGTAGTTGAACTGCAAAAGAAGATTAAAGCAGCTGACGATATTTTCCCGAACCAAAAGAAAAATCCCGACAAGAAAGATAACCCAAATGTTTTAGAAGTGTTAGGTGAATACGGTGGAGATGGTTTCTTTCCTACATTAGGTAATAACGGTTTAAACATCCCGGACCAAGTTTTCCAATCCTTTAATAACTTCCAAATTGAGAACTATAAGATTAGTGACTTTAAGGTGGACATTGCCAGTGAACGTGACATTGTCCAACACGACAAGTTCCGTTTTAGTTATGTCGTTAACATTGGTTTAGAGTTAGCACTTTTAGTCAATAAGCGTCCGGTTTACTTTAACTTTAGCTTGGATCTGCGGACCAATAACTTCTCCAACCAAGCCGGTTTTAACGAAATCTTTAATGCGCCTGGTAACGCAGCTACTAACTGA", "aa": "MAKVVELQKKIKAADDIFPNQKKNPDKKDNPNVLEVLGEYGGDGFFPTLGNNGLNIPDQVFQSFNNFQIENYKISDFKVDIASERDIVQHDKFRFSYVVNIGLELALLVNKRPVYFNFSLDLRTNNFSNQAGFNEIFNAPGNAATN", "aa_hexdigest": "e7988e96cf7e736d18cf425a3e5c8a27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 143, "aa_cov": 0.9794520547945206, "hmm_cov": 1.0, "evalue": 1.744144965732361e-66, "score": 219.39715576171875, "start": 2, "stop": 144 } ], "seq_stats": { "molecular_weight": 16523.340499999988, "isoelectric_point": 5.385744667053221 }, "id": "GIHJHMOALN_117", "locus": "GCA009939745_00077" }, { "type": "cds", "sequence": "contig_1", "start": 44300, "stop": 44662, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "GTGAAACAGTTGAACTATGATGGTAATGACAGTACCCATTTAGCCAATACCCTCTTACAAGATCAGTTCAATACCTTAAACTTAAACTTGCAAAAGTCGATCTATGAGTTGCCATTAACCGAAATGGAAACTAAGTTCCAGCAAGAATATGTGGAACTTTTACTAGCTAAACGTAGAGAGGAAAAACGACTTTGGGATGAAGAACAAAAACGAATTGAAGCAGAACGGAAACAAAAAGAAGCAGAGCTAGCACAAATTCAGCGTGAACTTAAAGAAAAGGCTGAGAAGGATGCGGCGGTTAAACAAGCTCAAACTAACTTAAAAACTGCTCTAAGTAGTGTGGAAGGCTTTGTGAAATTCTGA", "aa": "MKQLNYDGNDSTHLANTLLQDQFNTLNLNLQKSIYELPLTEMETKFQQEYVELLLAKRREEKRLWDEEQKRIEAERKQKEAELAQIQRELKEKAEKDAAVKQAQTNLKTALSSVEGFVKF", "aa_hexdigest": "1a8d0ee571f925de26a377aeb83b8535", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 34, "aa_cov": 0.2833333333333333, "hmm_cov": 1.0, "evalue": 9.785912314131707e-14, "score": 46.73021697998047, "start": 2, "stop": 35 } ], "seq_stats": { "molecular_weight": 14157.850599999985, "isoelectric_point": 5.581100273132324 }, "id": "GIHJHMOALN_118", "locus": "GCA009939745_00078" }, { "type": "cds", "sequence": "contig_1", "start": 45020, "stop": 45193, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "GTGAAAGCCACCATTGATGGTAATCCAAGTGATACCACCAAAGTATGGATTGCACCAGAAGACTTTAAACAGGGTGTACACATTGGTGTGGGGGAACACTTCAAAGGTAAGGCTTACAAGTTTAAGTTTGAAGTTAGTTTTGAATTAGAAGGCTCGATTGCAGCCCACTGGTGA", "aa": "MKATIDGNPSDTTKVWIAPEDFKQGVHIGVGEHFKGKAYKFKFEVSFELEGSIAAHW", "aa_hexdigest": "cc0b7608021588bb904448ec69e0ed11", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 56, "aa_cov": 0.9824561403508771, "hmm_cov": 0.9821428571428571, "evalue": 2.6039897871565532e-15, "score": 52.1909294128418, "start": 2, "stop": 57 } ], "seq_stats": { "molecular_weight": 6381.144599999998, "isoelectric_point": 6.247423744201661 }, "id": "GIHJHMOALN_119", "locus": "GCA009939745_00079" }, { "type": "cds", "sequence": "contig_1", "start": 45660, "stop": 45935, "strand": "-", "frame": 2, "gene": null, "product": "DUF16 family-like protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031ACD5D", "UniRef:UniRef100_A0AB38W8A6", "UniRef:UniRef50_A0AB38W8A6", "UniRef:UniRef90_A0AB38W8A6" ], "nt": "GTGAACACTGAATACGTAACGCGTAAAGAGTTTAACGAGTACAAAAAATCAAAAGACCAACGACTTACAAAGATTGAAACTGAGTTAAAGAGTCAGGGTCAAAGGATTCAAGTTGTTGAGGACAAAGTTGATCGACTAACTGAAGTTGTTATCGTTCAAGGCCAACAAATTAAAGAGCTTCAAGTTGAACAAAAAAATCAAGGAGAAACACTTAGATTAATTTTGAAAGCACTTGAAGGCATAAACAGACGTCTAGACAATTTGGAATCTAAATAA", "aa": "MNTEYVTRKEFNEYKKSKDQRLTKIETELKSQGQRIQVVEDKVDRLTEVVIVQGQQIKELQVEQKNQGETLRLILKALEGINRRLDNLESK", "aa_hexdigest": "02da2a5cb0267e820093654141b6962d", "start_type": "GTG", "rbs_motif": "AACAA", "ups": { "uniparc_id": "UPI00031ACD5D", "uniref100_id": "UniRef100_A0AB38W8A6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031ACD5D", "UniRef:UniRef100_A0AB38W8A6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8A6", "uniref90_id": "UniRef90_A0AB38W8A6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8A6", "product": "DUF16 family-like protein", "uniref50_id": "UniRef50_A0AB38W8A6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8A6" ], "product": "DUF16 family-like protein" }, "genes": [], "id": "GIHJHMOALN_120", "locus": "GCA009939745_00080" }, { "type": "cds", "sequence": "contig_1", "start": 46366, "stop": 46716, "strand": "-", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_038", "db_xrefs": [ "RefSeq:WP_010874395.1", "SO:0001217", "UniParc:UPI0000139F43", "UniRef:UniRef100_P75076", "UniRef:UniRef50_P75076", "UniRef:UniRef90_P75076" ], "nt": "TTGTTTAAGAAAAGACTAAATAAAGATAAGATTAATGATTGTTATACATGGGAAGAAGAACTACCTGATGGTAGCTACGACATGGGATTTCATGGCAATTTAAACCATATGGAAAAAGGTAAAAGTGGATACGTCACCCATAAACAACTGGATAAAAAACTAGAAGTGTTCAAACAAGATCTTCTAGTTGAACTTAGTGAAAAATTTGTAACTAAGGAAGAATTCAGAGCTCAGGGCAAGCAAATCAAAGAACTTCAAATTGAGCAAAAGGCTCAAGGCAAGACACTTCAATTAATACTCGAAGCTCTCCAGGGTATAAACAAACGTTTAGACAAATTGGAATCTAAGTAG", "aa": "MFKKRLNKDKINDCYTWEEELPDGSYDMGFHGNLNHMEKGKSGYVTHKQLDKKLEVFKQDLLVELSEKFVTKEEFRAQGKQIKELQIEQKAQGKTLQLILEALQGINKRLDKLESK", "aa_hexdigest": "a093b243fb02ab51931a319ab54c7036", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139F43", "ncbi_nrp_id": "WP_010874395.1", "uniref100_id": "UniRef100_P75076", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139F43", "RefSeq:WP_010874395.1", "UniRef:UniRef100_P75076" ] }, "ips": { "uniref100_id": "UniRef100_P75076", "uniref90_id": "UniRef90_P75076" }, "psc": { "uniref90_id": "UniRef90_P75076", "product": "UPF0134 protein MPN_038", "uniref50_id": "UniRef50_P75076" }, "pscc": { "uniref50_id": "UniRef50_P75076", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75076" ], "product": "UPF0134 protein MPN_038" }, "genes": [], "id": "GIHJHMOALN_121", "locus": "GCA009939745_00081" }, { "type": "cds", "sequence": "contig_1", "start": 47108, "stop": 47257, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAGGCGCCAGTGCGTTGTAAGGCAGTTGGAACCGGTTACTTATTGACATCAGTACTAGTAATAAGCAAACTAGAAAACGGTATAAGTGAGACAGAAAAGGTTAACCCCAGTAACAATTTAGGGAGTTTTGTCATTAATTATTAG", "aa": "MAEAPVRCKAVGTGYLLTSVLVISKLENGISETEKVNPSNNLGSFVINY", "aa_hexdigest": "2d7d6401867844d1a68f4c74c3dfd267", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5227.960300000001, "isoelectric_point": 6.04683895111084 }, "id": "GIHJHMOALN_122", "locus": "GCA009939745_00082" }, { "type": "cds", "sequence": "contig_1", "start": 47301, "stop": 47438, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGGAGCAAATTGAACAGCTCCAACAATTTACGCCAGCGCAGCGCTTTACCCAATTTAAAAAGAAATTTCCCAACCAAAAATTACTCAGTCAAAGTGAGTTAAGTCCAGTTGATGTTTACAATTTTTTAAGTGGTTGA", "aa": "MEQIEQLQQFTPAQRFTQFKKKFPNQKLLSQSELSPVDVYNFLSG", "aa_hexdigest": "0cfc38b0e33a12a594af4774dc28b39b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 41, "aa_cov": 0.9111111111111111, "hmm_cov": 1.0, "evalue": 1.6689268444270266e-13, "score": 45.97133255004883, "start": 5, "stop": 45 } ], "seq_stats": { "molecular_weight": 5319.996000000001, "isoelectric_point": 8.140181159973146 }, "id": "GIHJHMOALN_123", "locus": "GCA009939745_00083" }, { "type": "cds", "sequence": "contig_1", "start": 47448, "stop": 47906, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_039", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75075", "UniRef:UniRef90_P75075" ], "nt": "TTGGTCTCTTTCTTGGATAGAGTAATTAAATTACAAGGCAAGGTTAAGGAAGCAAATGAAATTTTTAATCCAAATGTAGGTGATCAAATAGTTTTACCTAAAAAGGAAAACCCGAATGTTTTGGAAGTATTGGGCGAGTATAACGGTTTTGGTTTCTTCCCTACATTGGGTAAAAACGGATTAAACCTACCACAGCAAATCTTTGAAAACTTTACTGACTTTAAAGTAGAGAGTTACCAAATTAACGATTTCAAGGTAAGTCTAGTGGGCGAGCGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAACTGCTTGTTAACAACCAAAAGGTTACTTTCAATATTACGGTTGATTTGAGAACCAATAACTTTTCTACCCAAGAGACTTTCAATGATTTATTCAACAATGGAACTGCACCAACCAACTGA", "aa": "MVSFLDRVIKLQGKVKEANEIFNPNVGDQIVLPKKENPNVLEVLGEYNGFGFFPTLGKNGLNLPQQIFENFTDFKVESYQINDFKVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNDLFNNGTAPTN", "aa_hexdigest": "49ae170815a8e828560d47f96ed40d8b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 47148, "stop": 48134, "observations": { "mutation": [ 47214, 47436, 47904 ], "stop": [ 47214, 47436, 47904 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75075", "query_cov": 1.0, "subject_cov": 0.44970414201183434, "identity": 0.993, "score": 301.0, "evalue": 5.47e-101, "gene_start": 1, "gene_end": 152, "reference_start": 111, "reference_end": 262, "reference_sequence": "MTKLPKLLLGLTFSVSLIPFSSLLITSTDVNKQPVPTALQRTGASAIHEGSFQTITLGQSLMEQIEQLQQFTPAQRFTQFKKKFPNQKLLSQSELSPVDVYNFLSGWQDALVSFLDRVIKLQGKVKEANEIFNPNVGDQIVLPKKENPNVLEVLGEYNGFGFFPTLGKNGLNLPQQIFENFTDFKVESYQINDFKVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNDLFNNGTAPTNWQFFSRIKVNKLHYDQTDATHLANTLLQDQFNALNLDLQKSIYDLNLDDLEERFEEEYAKPLREKRTQQKKEWEEE" }, "paralog": false, "identity": 0.9847560975609756, "score": 634.0, "evalue": 1.97e-234, "description": "Internal stop codon at 47214, 47436, 47904. Nonsense mutation around 47214, 47436, 47904.", "psc": { "uniref90_id": "UniRef90_P75075", "product": "Uncharacterized protein MPN_039", "uniref50_id": "UniRef50_P75075", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75075", "UniRef:UniRef50_P75075" ] }, "pscc": { "uniref50_id": "UniRef50_P75075", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75075" ], "product": "Uncharacterized protein MPN_039" } }, "id": "GIHJHMOALN_124", "locus": "GCA009939745_00084" }, { "type": "cds", "sequence": "contig_1", "start": 47937, "stop": 48134, "strand": "+", "frame": 3, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF9F4C5", "UniRef:UniRef90_UPI000AF9F4C5" ], "nt": "TTGCACTATGACCAAACTGATGCCACCCATTTAGCTAACACCCTACTACAAGACCAGTTTAATGCGTTAAACCTTGATTTACAAAAATCGATTTACGACCTTAACCTTGATGGTTTAGAAGAAAGGTTTGAAGAGGAATACGCTAAGCCACTTCGTGAAAAAAGAACTCAGCAAAAGAAAGAATGGGAAGAGGAATAG", "aa": "MHYDQTDATHLANTLLQDQFNALNLDLQKSIYDLNLDGLEERFEEEYAKPLREKRTQQKKEWEEE", "aa_hexdigest": "cccfa81d6f589aa24b73ad76951de46e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 47712, "stop": 48134, "observations": { "mutation": [ 47904 ], "stop": [ 47904 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF9F4C5", "query_cov": 1.0, "subject_cov": 0.4642857142857143, "identity": 0.985, "score": 125.0, "evalue": 1.65e-35, "gene_start": 1, "gene_end": 65, "reference_start": 76, "reference_end": 140, "reference_sequence": "MGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNDLFNNGTAPTNWQFFSRIKVNKLHYDQTDATHLANTLLQDQFNALNLDLQKSIYDLNLDGLEERFEEEYAKPLREKRTQQKKEWEEE" }, "paralog": false, "identity": 0.9857142857142858, "score": 278.0, "evalue": 1.95e-98, "description": "Internal stop codon at 47904. Nonsense mutation around 47904.", "psc": { "uniref90_id": "UniRef90_UPI000AF9F4C5", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000AF9F4C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF9F4C5", "UniRef:UniRef50_UPI000AF9F4C5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF9F4C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF9F4C5" ], "product": "DUF240 domain-containing protein" } }, "id": "GIHJHMOALN_125", "locus": "GCA009939745_00085" }, { "type": "cds", "sequence": "contig_1", "start": 48154, "stop": 48318, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAACTGCAAAAAGAAATTGAAGAACAACAAAAGAAACAAAGAGAACAAGCAAAAAACGATGCTGAGCTCAAAAAAGCACAAGATGATCTCAAAGTTGCGCTTAATAGTCTTGACGGTTTTGTTAAATTCTTTACATCAAACGAAGGTCGCTTTAAATTAG", "aa": "MLNCKKKLKNNKRNKENKQKTMLSSKKHKMISKLRLIVLTVLLNSLHQTKVALN", "aa_hexdigest": "736e452a7e7efcc8a81098d493c761a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6329.709699999999, "isoelectric_point": 11.003479957580566 }, "id": "GIHJHMOALN_126", "locus": "GCA009939745_00086" }, { "type": "cds", "sequence": "contig_1", "start": 48933, "stop": 49154, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "GTGCGTCCACCACAATACAGTTTTAAGGAACAAGAAAAACAACTTTTATTTGTACCACGTCATGTACAAAAAATTGAAGCTGAAGGTAAACACCTTGAAATAATTAATCCGTTCTTAAAAGATCAACATTTAGATTTTTTTGAACACTACCACCCAGATTTAACGCAGCCTTTAGACATGGTGTCATACCTAATGTATGCCATCGCTGACAAGGTGAAATAA", "aa": "MRPPQYSFKEQEKQLLFVPRHVQKIEAEGKHLEIINPFLKDQHLDFFEHYHPDLTQPLDMVSYLMYAIADKVK", "aa_hexdigest": "f62d6569114a02f37a7eac1ee64640fa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 27, "aa_cov": 0.3698630136986301, "hmm_cov": 0.9629629629629629, "evalue": 2.4440992384483764e-07, "score": 26.370834350585938, "start": 2, "stop": 28 } ], "seq_stats": { "molecular_weight": 8811.087799999996, "isoelectric_point": 6.16506404876709 }, "id": "GIHJHMOALN_127", "locus": "GCA009939745_00087" }, { "type": "cds", "sequence": "contig_1", "start": 49216, "stop": 49521, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGAAAAAACTCCACAAAATTTTATTGGGTTTATCTTTGCCCGCATCTTTGGCGCCCTTATCTGGGTTTATTAGTACTGATGCTGATAACTCACAGTTTTCACTAAAGAAGTCTAGCACTGAAATTAAAGGTACACAATCCATTCACCTGCTTAATTTAGGGGAAAGTTTAACTGAAGAAATTAAAGAGGCACAAAAGCGTACCCCAGAAACTAGTTTTGCCAGTTTCAAACAAAAGTTTCCCAATAAGGAAAGTTTTGTTAAAGGTTTTCAACCAATTGATGTCTACAACTTACTAAGTGGTTGA", "aa": "MKKLHKILLGLSLPASLAPLSGFISTDADNSQFSLKKSSTEIKGTQSIHLLNLGESLTEEIKEAQKRTPETSFASFKQKFPNKESFVKGFQPIDVYNLLSG", "aa_hexdigest": "d06a012651ff44db3191783cbd8a3a38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 40, "aa_cov": 0.38613861386138615, "hmm_cov": 1.0, "evalue": 2.813602965861801e-11, "score": 38.68211364746094, "start": 63, "stop": 101 } ], "seq_stats": { "molecular_weight": 11127.642499999985, "isoelectric_point": 9.260899543762207 }, "id": "GIHJHMOALN_128", "locus": "GCA009939745_00088" }, { "type": "cds", "sequence": "contig_1", "start": 49648, "stop": 50484, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_042", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078", "UniRef:UniRef90_P75072" ], "nt": "GTGCTAAACGTTTTGGGCTCATATGGTGGTGAAGGGTTCTTTCCTGCATTGGGTAAAAACGGGTTGAACCTACCACAACAAATCTTTGAAAACTTCACTGATTTCAAAGTTGTTAGTCATAAAATTCATGACTTCCAAGTAAGTTTGGTAGGCGAACGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAGCTGCTTGTTAACAACCAAAAAGTTACTTTCAACATTACGGTTGATTTGAGAACCAATAATTTCTCTACCCAAGAAACTTTTAACGAGCTGTTCAATAAATGCATTGGCCCCGTTAACTGGCAATTCTTTAGTAGGGTTAAAGTAGATAAATTGCACTACGATCAAACTGACGCTACTCATTTAGCTAACACGTTGTTACAAGACCAGTTCAATGCGTTAAATCTTGATTTAGAAAAGTCCATCTATGACTTGGAACTACCTAGATTAGAAGCAGAGTTCCAACAAAAATACGTTGATCCTTTAATTGAGAAAAAGCAAAGACAAAAAGCAGAGTGGGAAGAAGCGGAACGTATCCGTAAGGAAGAAGAGGAAAAACACCAAAAGGAGCTTGAAGAACAACAAAGAATTCAAGCAGAAAAAGCGAAAAATGACGAACAGTTACGTAAAGCGCAAACTGAATTGAAGAAGGCGCTTGGTGGCATCGACTCCTTTGTTGAATTCTTTACGAATAATGATTTACGTCTTAAACTAGGTTACACCAAAGAAGACAACGTTAGAACCTGCGCTGGACTTTTTAGAGCACTAGAAGTTTCCTTTGGTAATTACCGCGCTTGA", "aa": "MLNVLGSYGGEGFFPALGKNGLNLPQQIFENFTDFKVVSHKIHDFQVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNELFNKCIGPVNWQFFSRVKVDKLHYDQTDATHLANTLLQDQFNALNLDLEKSIYDLELPRLEAEFQQKYVDPLIEKKQRQKAEWEEAERIRKEEEEKHQKELEEQQRIQAEKAKNDEQLRKAQTELKKALGGIDSFVEFFTNNDLRLKLGYTKEDNVRTCAGLFRALEVSFGNYRA", "aa_hexdigest": "8a01af7c8284353185d688c913b9c618", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 49348, "stop": 50784, "observations": { "mutation": [ 49519, 50482, 50509 ], "stop": [ 49519, 50482, 50509 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75072", "query_cov": 1.0, "subject_cov": 0.41369047619047616, "identity": 0.978, "score": 503.0, "evalue": 1.48e-173, "gene_start": 1, "gene_end": 278, "reference_start": 145, "reference_end": 422, "reference_sequence": "MKKLHKILLGLSLPASLAPLSGFISTDADNSQFSLKKSSTEIKGTQSIHLLNLGESLTEEIKEAQKRTPETSFASFKQKFPNKESFVKGFQPIDVYNLLSGWKDAISSFLDKVVELQKKIEEANKIFNTNIGNQIDLPEDENPNVLNVLGSYGGEGFFPTLGKNGLNLPQQIFENFTDFKVVSHKIHDFQVSLVGERDIIKNDKVRFSYAVQIPLNLELLVNNQKVTFNITVDLRTNNFSTQETFNELFNKCIGPVNWQFFSRVKVDKLHYDQTDATHLANTLLQDQFNALNLDLEKSIYDLELPRLEAEFQQKYVDPLIEKKQRQKAEWEEAERIRKEEEEKHQKELEEQQRIQAEKAKNDEQLQKPQTELKKALGGIDSFVEFFTNNDLRLKLGYTKEDNVRTRAGLFRALEVSFGNYRAWTFYITLLGWKDTTEKIFKKAKWQDIRDDEKFRKAFGLSPKATEKDVGKVTNPGYGYQGIYIKDSLRDGIAKYSDSTVSEPKNVKVSLPGTVGDNEEGKIWIASHNFRQNHEWGAGEKFKYSAYRFKFDVTVDYDVEVSAKWWTWAFRGSIPGYWRGKFKVTYSFDGVVPSWKYGHIQVRTPQYSFNKQEQKILFVPHAIQKIAAEGSNLDLINPFLKDQKLDEFEHYHPDLTKPLDLVAYLLYAITTRS" }, "paralog": false, "identity": 0.9832635983263598, "score": 900.0, "evalue": 0.0, "description": "Internal stop codon at 49519, 50482, 50509. Nonsense mutation around 49519, 50482, 50509.", "psc": { "uniref90_id": "UniRef90_P75072", "product": "Uncharacterized protein MPN_042", "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75072", "UniRef:UniRef50_P75078" ] }, "pscc": { "uniref50_id": "UniRef50_P75078", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75078" ], "product": "Uncharacterized protein MPN_036" } }, "id": "GIHJHMOALN_129", "locus": "GCA009939745_00089" }, { "type": "cds", "sequence": "contig_1", "start": 51016, "stop": 51234, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "GTGCGTACGCCACAATACAGCTTTAATAAGCAGGAACAAAAGATTCTGTTTGTACCCCATGCTATCCAAAAGATTGCTGCTGAAGGTAGCAACTTAGATTTAATTAATCCATTCTTAAAGGATCAAAAATTAGATGAATTTGAGCACTACCACCCAGATTTAACTAAACCATTAGATTTAGTAGCTTATCTGTTATACGCAATAACCACGAGATCATAG", "aa": "MRTPQYSFNKQEQKILFVPHAIQKIAAEGSNLDLINPFLKDQKLDEFEHYHPDLTKPLDLVAYLLYAITTRS", "aa_hexdigest": "4b877f46287aea38b401af53343dd049", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 27, "aa_cov": 0.375, "hmm_cov": 0.9629629629629629, "evalue": 7.958802146160542e-08, "score": 27.948936462402344, "start": 2, "stop": 28 } ], "seq_stats": { "molecular_weight": 8417.559499999992, "isoelectric_point": 6.245775413513184 }, "id": "GIHJHMOALN_130", "locus": "GCA009939745_00090" }, { "type": "cds", "sequence": "contig_1", "start": 51558, "stop": 51725, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATTTAAGTGATTTTAGTGAATTACCACGTTGGATTGGTGCCGAATTTTTAGGCACATTCTTTTTAATTCTTTTAGGTAACGGTGCTGGTTCGCAGCTAACACTCAATAAGATGTTTGTTAAAGAATCGAAAGCAAAGTTACTAACTGCTGCTTTTGCTTGA", "aa": "MFNLSDFSELPRWIGAEFLGTFFLILLGNGAGSQLTLNKMFVKESKAKLLTAAFA", "aa_hexdigest": "649f67f9b4957bab2ab9da7fdf44e849", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6056.0573, "isoelectric_point": 8.190466499328615 }, "id": "GIHJHMOALN_131", "locus": "GCA009939745_00091" }, { "type": "cds", "sequence": "contig_1", "start": 51993, "stop": 52241, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTACCCATTGTACTAACCCAGCAATCTTTAACATTCCTCGCAACTTTGCAACTGAGTTTGTAGCAACTAGCGTTTTAATTGCTAGTTTACTGGTGGCTGGTAGTTTTGGTGCCAACCGATTTGACCAGTCACCACGAGGGGTTGTACCAATGTTAGTGGTTACTGGCTTAATTATGTCTTTTGGTGCCGCTACTGGTACTGCAATTAACCCAGCCCGTGATTTAGGTCCGAGAATTGTTTACTGA", "aa": "MVTHCTNPAIFNIPRNFATEFVATSVLIASLLVAGSFGANRFDQSPRGVVPMLVVTGLIMSFGAATGTAINPARDLGPRIVY", "aa_hexdigest": "f85be29309d75ea99616540dc29d6a91", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8622.974299999996, "isoelectric_point": 9.486087608337403 }, "id": "GIHJHMOALN_132", "locus": "GCA009939745_00092" }, { "type": "cds", "sequence": "contig_1", "start": 52323, "stop": 52799, "strand": "-", "frame": 2, "gene": "tdk", "product": "thymidine kinase", "db_xrefs": [ "EC:2.7.1.21", "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897", "KEGG:K00857", "SO:0001217", "UniRef:UniRef50_P47280", "UniRef:UniRef90_P75070" ], "nt": "GTGAAATCCCGTAACGGTCACAGTGATGAAGCCATTGAAATTAATTCACCTTTAGAAATCTATGACTACCTCACTAAAGATCGTTTTGATGTGGTTGCCATTGATGAAGCCCAATTCTTTTCGAGTGAAATTGTCGAAGTGGTTAAATCACTCAATGATCTCGGCATTAACGTCATTGTTTCTGGTTTAGACACGGACTTCCGCGCTGAACCCTTTGGTAGCATTCCCCAACTGTTAGCGATTGCCGACAAGATTTGTAAGTTAGATGCCGTTTGTAATGTCTGTGGTCAGTTAGCCCAACGCACCCAGCGCATAGTGTCCAAGAGCAACGAAACCGTTTTAATTGGTGATATTGAAGCTTATGAACCACGTTGTAAGTTGCACCACCACGTGCAGGGTAAGCCAGTTACAGTTAAGACCAAGCAGTTTAAAGAACGCTTTAAGGAACTAAACGATAACAGCCACAAGCACACCTAA", "aa": "MKSRNGHSDEAIEINSPLEIYDYLTKDRFDVVAIDEAQFFSSEIVEVVKSLNDLGINVIVSGLDTDFRAEPFGSIPQLLAIADKICKLDAVCNVCGQLAQRTQRIVSKSNETVLIGDIEAYEPRCKLHHHVQGKPVTVKTKQFKERFKELNDNSHKHT", "aa_hexdigest": "a4658b649b4d25227492bb57443cb055", "start_type": "GTG", "rbs_motif": null, "genes": [ "tdk" ], "truncated": "3-prime", "pseudogene": { "start": 52323, "stop": 52997, "observations": { "mutation": [ 52865, 52934 ], "stop": [ 52865, 52934 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75070", "query_cov": 1.0, "subject_cov": 0.7053571428571429, "identity": 0.9940000000000001, "score": 313.0, "evalue": 4.15e-107, "gene_start": 1, "gene_end": 158, "reference_start": 67, "reference_end": 224, "reference_sequence": "MFNYNWATMSFSQVFHQSPRGWIEVICGPMFSGKTEELLRKIKRWKLAKIPVIIFKPKIDTRQQHLVKSRNGHSDEAIEINSPLEIYDYLTKDRFDVVAIDEAQFFSSEIVEVVKSLNDLGINVIVSGLDTDFRAEPFGSIPQLLAIADKICKLDAVCNVCGQLAQRTQRIVSKSNETVLIGDIEAYEPRCKLHHHVQGKPVTVKTKQFKERFKELNDNSHKHT" }, "paralog": false, "identity": 0.9866071428571429, "score": 443.0, "evalue": 9.27e-161, "description": "Internal stop codon at 52865, 52934. Nonsense mutation around 52865, 52934.", "psc": { "uniref90_id": "UniRef90_P75070", "gene": "tdk", "product": "thymidine kinase", "ec_ids": [ "2.7.1.21" ], "uniref50_id": "UniRef50_P47280", "kegg_orthology_id": "K00857", "go_ids": [ "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75070", "EC:2.7.1.21", "UniRef:UniRef50_P47280", "KEGG:K00857", "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897" ] }, "pscc": { "uniref50_id": "UniRef50_P47280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47280" ], "product": "Thymidine kinase" } }, "id": "GIHJHMOALN_133", "locus": "GCA009939745_00093" }, { "type": "cds", "sequence": "contig_1", "start": 53132, "stop": 53527, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30", "PFAM:PF01409.25", "PFAM:PF13393.11" ], "nt": "TTGGAATACGCTGAAGCCTTTAAGCGCACCAATGCCAACGCCGATATTGTCAAGAAGGAGTTGTATGAATTCCATGACAAATCGAACCGGCTGTTAGCCCTGCGTCCCGAGGCTACTGCTGGGATAGTGCGCTTGGTCTGTGAGAACAAACTACTCCAACCACAAAACTACCCGTTAAGGTTGTTTACCATTGGCACCATGTACCGGTATGAACGCCCCCAAAGTAACCGTTACCGCGAGCACTACCAGTTTAGTTGTGAAGTGATAGGTGACACTAATCCAACCGTGTTACTCGACACTTTACTGTTGGGTCATGCCATTATCCAGCAATTGGGAATTGAAGGAGTGATCCTCAAACTTAACAACTTAGGTAACAGTGCTACCATTCAACAATGA", "aa": "MEYAEAFKRTNANADIVKKELYEFHDKSNRLLALRPEATAGIVRLVCENKLLQPQNYPLRLFTIGTMYRYERPQSNRYREHYQFSCEVIGDTNPTVLLDTLLLGHAIIQQLGIEGVILKLNNLGNSATIQQ", "aa_hexdigest": "250c2e5a0a23376fea71c4fd1bd25ebd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 105, "aa_cov": 0.7557251908396947, "hmm_cov": 0.9904761904761905, "evalue": 1.1522626062207222e-12, "score": 43.79168701171875, "start": 22, "stop": 120 }, { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 74, "aa_cov": 0.549618320610687, "hmm_cov": 0.9324324324324325, "evalue": 1.3081305322924564e-05, "score": 20.253562927246094, "start": 21, "stop": 92 }, { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 123, "aa_cov": 0.9389312977099237, "hmm_cov": 0.9512195121951219, "evalue": 1.9628744107941551e-19, "score": 65.43927001953125, "start": 2, "stop": 124 } ], "seq_stats": { "molecular_weight": 15053.090699999984, "isoelectric_point": 7.7830907821655275 }, "id": "GIHJHMOALN_134", "locus": "GCA009939745_00094" }, { "type": "cds", "sequence": "contig_1", "start": 53741, "stop": 54040, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "GTGCCGTTTGAGTGGAATCCAACCCTAGTCCGGGGCTTGGACTACTATACTGGGGTAGTGTTTGAATTTGTTAAGGATGACACCACGGTACTAGCTGGTGGGGTGTATGATAACCTAGTGGAAGAGTTAGGTGGTACACCTACTAAAGCGCTGGGTTTTGCTTGTGGCATTGAACGCTCAATTAATTGTTTAAGTGCGGTTAAAAAGCAAGCAATTTTGGCCAATCAACCCCCACGATTACTGGTTATAGGTCTCACCGAAGCAGCGTTGGAAAAGCTGTTACAACTTTCATTGGGTTGA", "aa": "MPFEWNPTLVRGLDYYTGVVFEFVKDDTTVLAGGVYDNLVEELGGTPTKALGFACGIERSINCLSAVKKQAILANQPPRLLVIGLTEAALEKLLQLSLG", "aa_hexdigest": "200267869656c59ceb5b281052b89fee", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 57, "aa_cov": 0.5555555555555556, "hmm_cov": 0.9824561403508771, "evalue": 7.649910983342461e-13, "score": 43.79265213012695, "start": 4, "stop": 58 } ], "seq_stats": { "molecular_weight": 10683.288399999989, "isoelectric_point": 4.761254692077636 }, "id": "GIHJHMOALN_135", "locus": "GCA009939745_00095" }, { "type": "cds", "sequence": "contig_1", "start": 54056, "stop": 54214, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "GTGACTATTTATCCTAAAGTTATTAGAATTATCAATGGCATCAGAGCCGCGCAACGGTTAGGTTACCGCTTTTTAGGGGTCATTGGTGGTAATAATTTAGAACAGCAAACCATTACCGTTAAAGACCTGGCTACTGAACAGCAAACAACATACACTTGA", "aa": "MTIYPKVIRIINGIRAAQRLGYRFLGVIGGNNLEQQTITVKDLATEQQTTYT", "aa_hexdigest": "edd2793ff4e7153f8d48217d15fe296e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 39, "aa_cov": 0.75, "hmm_cov": 1.0, "evalue": 2.9359740939355375e-07, "score": 26.083879470825195, "start": 12, "stop": 50 } ], "seq_stats": { "molecular_weight": 5868.7206000000015, "isoelectric_point": 9.815069770812986 }, "id": "GIHJHMOALN_136", "locus": "GCA009939745_00096" }, { "type": "cds", "sequence": "contig_1", "start": 54216, "stop": 54659, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAATTTAGACAACGCCAAGTGCTTTAAGCAACGGGTTTTTATTGGCAATTTAACTTCCGAGCACCTCAATAAAACCGTTACTATTGCCGGATGGGTCAAACGGATTAAGAAGTTAGGTGAACTTAACTTTGTTATTGTCGGGGACAAGACTAATACTATTCAGGTTACCTGTAAAAATAAGGAACAGGTCAAGTACTTAACCAGGGAAGACTTGGTGATTGTCAAGGGCAAACTGAAACGCTTAGACAGTGTTCGGTTTGAAATTACCAATCCTACTATTACCTTATTTGCCAAGTCCAAAACACCCCCTCTTATCATTGAGGACAAAACTGATGCTTTAGAAGAAGTGCGTTTGCGTTACCGCTACTTAGACTTACGCCGTCCGGTGATGCAAAAGCGCTTGGCGTTACGCCATAAGGTAACTTTAGCCGTCCGGAACTGA", "aa": "MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKNKEQVKYLTREDLVIVKGKLKRLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLRYRYLDLRRPVMQKRLALRHKVTLAVRN", "aa_hexdigest": "4494097b7192b7952a9407b945689d6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 74, "aa_cov": 0.47619047619047616, "hmm_cov": 1.0, "evalue": 1.82077624307953e-15, "score": 52.1613655090332, "start": 27, "stop": 96 } ], "seq_stats": { "molecular_weight": 17120.06379999999, "isoelectric_point": 10.203362846374514 }, "id": "GIHJHMOALN_137", "locus": "GCA009939745_00097" }, { "type": "cds", "sequence": "contig_1", "start": 54669, "stop": 55346, "strand": "+", "frame": 3, "gene": "aspS", "product": "aspartate--tRNA ligase", "db_xrefs": [ "COG:COG0173", "COG:J", "EC:6.1.1.-", "EC:6.1.1.12", "GO:0003676", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422", "SO:0001217", "UniRef:UniRef50_P75068", "UniRef:UniRef90_P75068" ], "nt": "ATGGGGTTTATTGAAGTAGAAACACCTACGCTCACGAAGTCCACACCGGAGGGTGCTAGGGACTTTTTGGTACCGGCCAGAATTAGGGAACATAGCTTTTACGCTTTGCCCCAAAGTCCACAAATTTATAAACAGTTATTAATGGTTGGTGGTACGGAAAAGTACTTCCAAATTGCCCATGTTTACCGCGATGAAGACAGCCGCAAAGACCGTCAACCTGAACACACCCAAATTGACTTAGAGGTGGCCTTTTACACCAAGGAAATGGTGATGGATTTAATCCAAAGGTTATTTGTCGATGTCTTTCGCCAAGTGTTAAACATTAAGCTCAAAAAACCGTTTCCAGTGTTAAAGTTTGCTGAAGCCTTTAACCGTTTTGGCAGTGACAAACCGGATTTACGTTATGGTTTCGAACTGGAAGACTGCACTGATTTATTCCAAGACAGTCCGAACCAATTCACTAACTTAATTAATGCTGGCGGCATAGTCGGTGGGATCCAATTACCGAATCTTTATTTAGACGAAGTTTCTTTTAAAGCATTGAGGAAACTAGCTAAGGACAACGGGGTTTCCTTAGAGTTTTACTCTGATAAAGCTAGTTCCCTAAAACAACCCCTGGATTTACCGTTAGCAGGCACCATCTTACTGGTAGCACACAAAAGTAAAACGCAAGCTTGA", "aa": "MGFIEVETPTLTKSTPEGARDFLVPARIREHSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEMVMDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSPNQFTNLINAGGIVGGIQLPNLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPLAGTILLVAHKSKTQA", "aa_hexdigest": "3eb50f3ccdf669972a263758bb687614", "start_type": "ATG", "rbs_motif": null, "genes": [ "aspS" ], "truncated": "both", "pseudogene": { "start": 54369, "stop": 55649, "observations": { "mutation": [ 54657, 55344, 55422 ], "stop": [ 54657, 55344, 55422 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75068", "query_cov": 1.0, "subject_cov": 0.40394973070017953, "identity": 1.0, "score": 448.0, "evalue": 2.88e-154, "gene_start": 1, "gene_end": 225, "reference_start": 152, "reference_end": 376, "reference_sequence": "MNLDNAKCFKQRVFIGNLTSEHLNKTVTIAGWVKRIKKLGELNFVIVGDKTNTIQVTCKNKEQVKYLTREDLVIVKGKLKRLDSVRFEITNPTITLFAKSKTPPLIIEDKTDALEEVRLRYRYLDLRRPVMQKRLALRHKVTLAVRNWLDQMGFIEVETPTLTKSTPEGARDFLVPARIREHSFYALPQSPQIYKQLLMVGGTEKYFQIAHVYRDEDSRKDRQPEHTQIDLEVAFYTKEMVMDLIQRLFVDVFRQVLNIKLKKPFPVLKFAEAFNRFGSDKPDLRYGFELEDCTDLFQDSPNQFTNLINAGGIVGGIQLPNLYLDEVSFKALRKLAKDNGVSLEFYSDKASSLKQPLDLPLAGTILLVAHKSKTQAWTALGAIRNELKYHLNLVKPNQYSFCWIVDFPLYEFDEKEQKWVSAHNMFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGVDPQQFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVDIKDITTIWSKHPVK" }, "paralog": false, "identity": 0.9929577464788732, "score": 839.0, "evalue": 8.21e-311, "description": "Internal stop codon at 54657, 55344, 55422. Nonsense mutation around 54657, 55344, 55422.", "psc": { "uniref90_id": "UniRef90_P75068", "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.-", "6.1.1.12" ], "uniref50_id": "UniRef50_P75068", "cog_id": "COG0173", "cog_category": "J", "go_ids": [ "GO:0003676", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75068", "EC:6.1.1.-", "EC:6.1.1.12", "UniRef:UniRef50_P75068", "COG:COG0173", "COG:J", "GO:0003676", "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422" ] }, "pscc": { "uniref50_id": "UniRef50_P75068", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75068" ], "product": "Aspartate--tRNA ligase" } }, "id": "GIHJHMOALN_138", "locus": "GCA009939745_00098" }, { "type": "cds", "sequence": "contig_1", "start": 55488, "stop": 55889, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGTTCTCCAATCCCCAACCGCAGTGGTTGGTTAACTTTGAAAACCACAAGGCTGAGGCGTTGAGTGAACAGTACGACCTAGTTTTAAATGGGTTTGAACTGGGTAGTGGTTCAATTCGGATCCATGACCCAGAAGTACAAACCCGTTTAATGCAATCTTTAGGGGTGGATCCCCAACAGTTTGGCTTTGTGATGGAGGCTTACCAGTACGGTGCTCCTGTACACGCTGGCATGGGTTTGGGATTAGACCGCTTAATGATGATCATTAACAATGTGGACAACATTAGGGAAGTGATGGCCTTTCCGAAAAATGCCCAAGGGATAGAGATGCACACTAATGCCCCTGATCAGGTTGACATTAAAGACATAACAACAATATGGTCCAAACACCCAGTGAAATAA", "aa": "MFSNPQPQWLVNFENHKAEALSEQYDLVLNGFELGSGSIRIHDPEVQTRLMQSLGVDPQQFGFVMEAYQYGAPVHAGMGLGLDRLMMIINNVDNIREVMAFPKNAQGIEMHTNAPDQVDIKDITTIWSKHPVK", "aa_hexdigest": "23ff0b70d9f50eeac3df90f955241f03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 93, "aa_cov": 0.6691729323308271, "hmm_cov": 1.0, "evalue": 3.530619135130408e-33, "score": 110.4085464477539, "start": 16, "stop": 104 } ], "seq_stats": { "molecular_weight": 15009.934499999992, "isoelectric_point": 5.019872093200683 }, "id": "GIHJHMOALN_139", "locus": "GCA009939745_00099" }, { "type": "cds", "sequence": "contig_1", "start": 55865, "stop": 56026, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCCAAACACCCAGTGAAATAAATACCCACCTTAAACACCTCTTGGCCTGTGATGCTTATAAGCTATCACACCGCTTAATGTATCCTAATGACACGACCAACTTATACAGTTGTTTAACAGCCCGGGGTGGTCGTGGTGGTTTTCCAAATTTTGTGTGA", "aa": "MVQTPSEINTHLKHLLACDAYKLSHRLMYPNDTTNLYSCLTARGGRGGFPNFV", "aa_hexdigest": "a54b422c165d3831a2a41c843d2a73cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5953.767000000001, "isoelectric_point": 8.770359611511232 }, "id": "GIHJHMOALN_140", "locus": "GCA009939745_00100" }, { "type": "cds", "sequence": "contig_1", "start": 56060, "stop": 56371, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGGTAACTTTTGTGATAGTGTGTTAGCAGTCCAAAACGATCCTGGGTTGGCCCAAGCGCTCACTGACAAGGTGACCACTGTTTTTGGTGATCCCCAGTTTGGGTTGGAGTTTACCCAGCACATCTGTTATTTAGCTAACTTTTTAAAACAACACCACCAGTTACCCTTAACGGTAAAGATTCACCAGAGTTCTGAGGGTTTAGCCTTCCGTACACCCTTAGTAACTATTACAGGTTCGGACCAAATGGTTCCCGAGTTAGTTTGGTTAGTCAACTATTTTGAAACCGTATTATTGGAAAACATCTGA", "aa": "MFGNFCDSVLAVQNDPGLAQALTDKVTTVFGDPQFGLEFTQHICYLANFLKQHHQLPLTVKIHQSSEGLAFRTPLVTITGSDQMVPELVWLVNYFETVLLENI", "aa_hexdigest": "e8f9f37f8bfa9a4aa3e2a29b4e605e02", "start_type": "GTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11552.11759999999, "isoelectric_point": 4.8213903427124025 }, "id": "GIHJHMOALN_141", "locus": "GCA009939745_00101" }, { "type": "cds", "sequence": "contig_1", "start": 56372, "stop": 57127, "strand": "+", "frame": 2, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:2.4.2.12", "EC:6.3.4.21", "GO:0004514", "GO:0009435", "GO:0047280", "SO:0001217", "UniRef:UniRef50_P75067", "UniRef:UniRef90_P75067" ], "nt": "TTGTACCAAACTACTTTAACTGTCGCCCAAAGCTTAAAGCTGCTGTTGGAACGCTATGCTAACGAAACCGCGGATAATACTGAATTTACCCACTTTCAGTGTCATGATTTTAGTATGCGTGGTATGAGTAGTCTTCAAAGTGCTTTATATGTAGCTAATGCTCACCTGCAGTACTTTAGTGGCAGTGACACCATTTTAGGCGGTGTAGCTGCTAAATCGATCTTAGCTAGTGAACACTCGGTGATGTGTGCTGATGGTCAAGAGGGTGAATTGAATACCTTTAAACGTTTATTGGAGCAGTTTCCTAACAAAAATCTGTCCTTAGTGATTGATTCTTATGACATGTGGCATGTTCTAGATAACATCTTACCCCAACTCAAGGACTTAGTTTTACAACGACAGGAGAAGCTGTACCTCCGACCTGACTCGGGCAACTTTGAAACCCTTATTTGTCAGGGCAAACGGTTTAACCCCGAGGACAAAACTACCTGGGGAGTAATTGATTACTTAGATTACCACTTTGGTTCCACTGTTAACCAAAAAGGTTATAAGGTTTTGAACCAAAAGTTGGGTATTGTTTATGGCGATGGCATTACCTATGAGCGGATTGAGTACATTTTGGAACAGCTCAAACAACGTGGGTTTTGTTCTTCCAACATTGTCTTTGGGGTTGGTAGTACAACTTACCAAAACCTTAACCGTGATACGTTGGGATTTGTGTATAAGTTGACAGCCATCAAAAAGGGTAATACCTGA", "aa": "MYQTTLTVAQSLKLLLERYANETADNTEFTHFQCHDFSMRGMSSLQSALYVANAHLQYFSGSDTILGGVAAKSILASEHSVMCADGQEGELNTFKRLLEQFPNKNLSLVIDSYDMWHVLDNILPQLKDLVLQRQEKLYLRPDSGNFETLICQGKRFNPEDKTTWGVIDYLDYHFGSTVNQKGYKVLNQKLGIVYGDGITYERIEYILEQLKQRGFCSSNIVFGVGSTTYQNLNRDTLGFVYKLTAIKKGNT", "aa_hexdigest": "4cdaf15c782691db99387183e9b5587d", "start_type": "TTG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "both", "pseudogene": { "start": 56072, "stop": 57220, "observations": { "mutation": [ 56369, 57125 ], "stop": [ 56369, 57125 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75067", "query_cov": 1.0, "subject_cov": 0.5565410199556541, "identity": 0.996, "score": 507.0, "evalue": 1.38e-178, "gene_start": 1, "gene_end": 251, "reference_start": 170, "reference_end": 420, "reference_sequence": "MVQTPSEINTHLKHLLACDAYKLSHRLMYPNDTTNLYSCLTARGGRGGFPNFVWNHEFAKKIILEVFGNFCDSVLAVQNDPGLAQALTDKVTTVFGDPQFGLEFTQHICYLANFLKQHHQLPLTVKIHQSSEGLAFRTPLVTITGSDQMVPELVWLVNYFETVLLENIWLYQTTLTVAQSLKLLLERYANETADNTEFTHFQCHDFSMRGMSSLQSALYVANAHLQYFSGSDTILGGVAAKSILASEHSVMCADGQEGELNTFKRLLEQFPNKNLSLVIDSYDMWHVLDNILPQLKDLVLQRQEKLYLRPDSGNFETLICQGKRFNPEDKTTWGVIDYLDYHFGSTVNQKGYKVLNQKLGIVYGDGITYERIEYILEQLKQRGFCSSNIVFGVGSTTYQNLNRDTLGFVYKLTAIKKGNTWHDVTKSPITDPTKQSIGGRFDNPNLIQVYG" }, "paralog": false, "identity": 0.9947643979057592, "score": 768.0, "evalue": 4.7e-284, "description": "Internal stop codon at 56369, 57125. Nonsense mutation around 56369, 57125.", "psc": { "uniref90_id": "UniRef90_P75067", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "2.4.2.12", "6.3.4.21" ], "uniref50_id": "UniRef50_P75067", "cog_id": "COG1488", "cog_category": "H", "go_ids": [ "GO:0004514", "GO:0009435", "GO:0047280" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75067", "EC:2.4.2.12", "EC:6.3.4.21", "UniRef:UniRef50_P75067", "COG:COG1488", "COG:H", "GO:0004514", "GO:0009435", "GO:0047280" ] }, "pscc": { "uniref50_id": "UniRef50_P75067", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75067" ], "product": "Nicotinamide phosphoribosyltransferase" } }, "id": "GIHJHMOALN_142", "locus": "GCA009939745_00102" }, { "type": "cds", "sequence": "contig_1", "start": 57525, "stop": 57680, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGCATTGCGCAACAACAGTAGTTTAAGCCGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGCCTTTGCTAAACAAAAGGATTGA", "aa": "MSQHGSSTDPSFVALRNNSSLSRDGKSRLLEFGNDLGFSKEETDAFAKQKD", "aa_hexdigest": "864a58654bf19d804a07fed2c7e58588", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5607.013900000001, "isoelectric_point": 5.658912849426269 }, "id": "GIHJHMOALN_143", "locus": "GCA009939745_00103" }, { "type": "cds", "sequence": "contig_1", "start": 57810, "stop": 58235, "strand": "+", "frame": 3, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C", "UniRef:UniRef90_UPI000A7C0B9C" ], "nt": "ATGAACGAGTTGCAAACCAAAGTCAATGAGGCTAATAATATTTTCCCAGTTGAAGCCTTCAAGGTACCTAAGGTTCCAGAAAAACTGTTTGGTTTTGTCAACCAAGGCTTCTTTCCTAAACTAAACCCTAAGGGCTTAAACATTGCTGATAACGTGGCTTCCTTATTTGAACAATACAGCTTAAAGCAAGCAAGTCTTAAGGACTTTGACATATTATTGGAAAAGAAAAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTAACTTTGAAACCACTTATGTTGGTACTGGTGGGGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACTACTAACTTTAGTAGCTTAGAAGAACTCCAAGCTAGCTTTAGTAAAACAGGCGATAACCTTACTGCCCAATTGTTCTGA", "aa": "MNELQTKVNEANNIFPVEAFKVPKVPEKLFGFVNQGFFPKLNPKGLNIADNVASLFEQYSLKQASLKDFDILLEKKNDIVLEHKVRYNFALQFNFETTYVGTGGEINLQFALQASTTNFSSLEELQASFSKTGDNLTAQLF", "aa_hexdigest": "5ba7808a9df5895b500f696b61ed0ffa", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 57525, "stop": 58265, "observations": { "mutation": [ 57678, 58233 ], "stop": [ 57678, 58233 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A7C0B9C", "query_cov": 1.0, "subject_cov": 0.5145985401459854, "identity": 0.9790000000000001, "score": 273.0, "evalue": 5.33e-91, "gene_start": 1, "gene_end": 141, "reference_start": 96, "reference_end": 236, "reference_sequence": "MSQHGSSTDPSFVVLRNNSSLSRDGKSRLLEFGNDLGFSKEETDAFAKQKDWNQNFVKFQKQFENKLLDPKNFSLTDVYNLFSGFQQSVTATVQLMNELQTKVNEANNIFPVEAFKVPKVPEKLFGFVNQGFFPKLNPKGLNIADNVASLFEQYSLKQASLKDFDILLEKKNDIVLEHKVRYNFALQFHFETTYIGSGGEINLQFALQASTTNFSSLEELQASFSKTGDNLTAQLFWKPTVTKLVSTLKPLMLVLVGKLTYSLHCKLPLLTLVA" }, "paralog": false, "identity": 0.9715447154471545, "score": 471.0, "evalue": 3.26e-171, "description": "Internal stop codon at 57678, 58233. Nonsense mutation around 57678, 58233.", "psc": { "uniref90_id": "UniRef90_UPI000A7C0B9C", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000A7C0B9C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A7C0B9C", "UniRef:UniRef50_UPI000A7C0B9C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A7C0B9C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C" ], "product": "DUF240 domain-containing protein" } }, "id": "GIHJHMOALN_144", "locus": "GCA009939745_00104" }, { "type": "cds", "sequence": "contig_1", "start": 58434, "stop": 58553, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCATTAAGAAAGCTGAAGAAGAGAAAATTAAAAAAGAACTTGAAGAGCAAAAGAAGCGTCAAGAAGAATTAGCAAAACAACAACGAGACAAAGAGGCATTACAAAAGTCACTTTGA", "aa": "MAIKKAEEEKIKKELEEQKKRQEELAKQQRDKEALQKSL", "aa_hexdigest": "39fbb92d0982d849d79b07c8a791bc5e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4711.3996, "isoelectric_point": 9.04905643463135 }, "id": "GIHJHMOALN_145", "locus": "GCA009939745_00105" }, { "type": "cds", "sequence": "contig_1", "start": 58671, "stop": 58940, "strand": "+", "frame": 3, "gene": null, "product": "DUF237 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AE7A69C", "UniRef:UniRef90_UPI000AE7A69C" ], "nt": "TTGTTAATTGCCGGTTTTAGAAGTGCAATTCAGACTTATTACAAAGATGGCAAAGCAGATCAAAGCCAAAACGCTAAAATTGCTTTTGGCGAAAAGGGTATTCAATTCCCTAAATCTGGCCCAGGACTTGACGGTATTTTCATGTCAGATTTCCTTCGTGGTAACCTTACCGGCAACGCTCACTTTGATTTAAAACTTAAAAAAGTTGAAGTTAAAAACACACAAGGAAAAGACGCACAAGGCAATGATAAAAAAGCCTCCATTAATTGA", "aa": "MLIAGFRSAIQTYYKDGKADQSQNAKIAFGEKGIQFPKSGPGLDGIFMSDFLRGNLTGNAHFDLKLKKVEVKNTQGKDAQGNDKKASIN", "aa_hexdigest": "4fee2ed8116e4876e8dbdb85b3183d26", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 58671, "stop": 59243, "observations": { "mutation": [ 58938, 59088 ], "stop": [ 58938, 59088 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AE7A69C", "query_cov": 1.0, "subject_cov": 0.43842364532019706, "identity": 1.0, "score": 178.0, "evalue": 3.19e-55, "gene_start": 1, "gene_end": 89, "reference_start": 1, "reference_end": 89, "reference_sequence": "MLIAGFRSAIQTYYKDGKADQSQNAKIAFGEKGIQFPKSGPGLDGIFMSDFLRGNLTGNAHFDLKLKKVEVKNTQGKDAQGNDKKASINWQAKQNNFPFRQVNPWDFSFEVELKYEGSYGLYPGARFLNLFGSLGIPNDWKGEMSVKFVLDGKTPQWIADKPDYPGSLFKFEKNQLKFTPHVKEHVHVENKQFMEKLKESKLA" }, "paralog": false, "identity": 0.9842105263157894, "score": 387.0, "evalue": 7.46e-140, "description": "Internal stop codon at 58938, 59088. Nonsense mutation around 58938, 59088.", "psc": { "uniref90_id": "UniRef90_UPI000AE7A69C", "product": "DUF237 domain-containing protein", "uniref50_id": "UniRef50_UPI000AE7A69C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AE7A69C", "UniRef:UniRef50_UPI000AE7A69C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AE7A69C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AE7A69C" ], "product": "DUF237 domain-containing protein" } }, "id": "GIHJHMOALN_146", "locus": "GCA009939745_00106" }, { "type": "cds", "sequence": "contig_1", "start": 59100, "stop": 59282, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAGCGTTAAGTTTGTGCTCGATGGTAAGACACCTCAATGGATTGCTGATAAACCAGATTACCCAGGTTCCTTGTTTAAATTTGAAAAGAACCAGTTAAAGTTCACTCCCCACGTTAAGGAGCATGTACATGTTGAAAACAAACAATTTATGGAAAAGTTGAAAGAGTCAAAACTTGCATAA", "aa": "MSVKFVLDGKTPQWIADKPDYPGSLFKFEKNQLKFTPHVKEHVHVENKQFMEKLKESKLA", "aa_hexdigest": "b182c67bca38873455c7b539de984f60", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 48, "aa_cov": 0.7833333333333333, "hmm_cov": 1.0, "evalue": 5.035702527474922e-18, "score": 60.979270935058594, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 7073.1571, "isoelectric_point": 9.302739524841307 }, "id": "GIHJHMOALN_147", "locus": "GCA009939745_00107" }, { "type": "cds", "sequence": "contig_1", "start": 59544, "stop": 60332, "strand": "+", "frame": 3, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C" ], "nt": "ATGCGCTTTCGGTATAAGGCGTTTCTACTAACTTTATTTGCGTCAACCACAACGCTTACTGGATTCATAATACCTACTTTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGCATTGCGCAACAACAGTAGTTTAAGCTGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGTGTTAGCGAAACAGAAAAACTGGGAGCAGAATTACACTAACTTTAAAAAGCAGTTTGAACACAAGCTTTTAGATCCGAAGAGCTTTAGCTTAACTGATGTGTATAACCTATTTAGTGGTTTTCAACAAAGTGTAGCTGACACGGTTAAGCTAATGAATGAATTGCAAACTCAGGTAAATAAAGCTAACGACATTTACCCAGTGGAAAGCTTTCAAGTGCCTAAGGTACCACAAAAGCTATTTGGATTTGTCGACCAGGGCTTCTTTCCGAAGTTAAACCCGAAGGGCTTGAACATTGCTGATAACGTGGCTTACTTGTTTGAGAAGTACAGCCTAAAACAAGCAACCCTTAAAGACTTTGACATCGTTTTGGAAAAGAAGAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTCACTTTGAAACTACATACATAGGTAGCGGTGGCGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACCACTAACTTTAGTAGTTTAGAGGAATTGCAAGCTAGCTTTAGTAAGGTAGGTAATAATTTAACTGCTCAATTGTTCTGA", "aa": "MRFRYKAFLLTLFASTTTLTGFIIPTLSQHGSSTDPSFVALRNNSSLSCDGKSRLLEFGNDLGFSKEETDVLAKQKNWEQNYTNFKKQFEHKLLDPKSFSLTDVYNLFSGFQQSVADTVKLMNELQTQVNKANDIYPVESFQVPKVPQKLFGFVDQGFFPKLNPKGLNIADNVAYLFEKYSLKQATLKDFDIVLEKKNDIVLEHKVRYNFALQFHFETTYIGSGGEINLQFALQASTTNFSSLEELQASFSKVGNNLTAQLF", "aa_hexdigest": "e14ddca2b71544650656a86e88412dcd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A7C0B9C", "query_cov": 0.9007633587786259, "subject_cov": 0.8613138686131386, "identity": 0.873, "score": 409.0, "evalue": 2.66e-142, "valid": false, "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000A7C0B9C" }, "pscc": { "uniref50_id": "UniRef50_UPI000A7C0B9C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A7C0B9C" ], "product": "DUF240 domain-containing protein" }, "genes": [], "id": "GIHJHMOALN_148", "locus": "GCA009939745_00108" }, { "type": "cds", "sequence": "contig_1", "start": 60759, "stop": 61013, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTCAATTATTAACTGCTGGAATGCGTGAAGGTATTAAGGAGTATTACAAACATAATAAACCTGACCAAAGCGCTAATGCTAAAAAAGCCTTTGGCCAAAATGGTCTTGCATTTCCGCGAACTGGCTTTGATGGTATCTACATGTCTGACTGGCTTCGTGGTGAATTGAGGAATAAGGGTAACATCAATCTCCATCTCAAACAAAACGAAACCACCGTTAAAAAGATTAGGGATGACATTAGTATCGAATGA", "aa": "MFQLLTAGMREGIKEYYKHNKPDQSANAKKAFGQNGLAFPRTGFDGIYMSDWLRGELRNKGNINLHLKQNETTVKKIRDDISIE", "aa_hexdigest": "52118a002728cc58b72aebcc472f0521", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9658.885399999994, "isoelectric_point": 9.520384788513184 }, "id": "GIHJHMOALN_149", "locus": "GCA009939745_00109" }, { "type": "cds", "sequence": "contig_1", "start": 62043, "stop": 62441, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02782.22" ], "nt": "ATGTACTCGGCAGCTGAAAGTGACTTTTATGCTAAGCTAGCACAAAAGGAAGAACAGGAAGTGGTGTTTGTCCCAGCCTTCACTGGTTTAGGCGCACCTTACTGGGATGCTTCAGCAAGAGGCGCCATCTTTGGGATAGAAGCTAATACCAAAAGGGAACATTTAGTAAAAGCTACCTTGGAAGCAATTGCCTTTCAAGCTAATGACCTGATTAAGGCAATGGCTAGTGATTTAAACAGTTCTATTAAAAAAATTAAGGCTGATGGCGGTGCTTGCAATTCCAACTATTTAATGCAATTTCAAGCTGATATTGCTAACTTAGAGGTGATTATTCCTAAGAATGTGGAAACCACTGCCATGGGTGCGGCCTTCCTAGCTGGTTTAGCAGTTAATTATTGA", "aa": "MYSAAESDFYAKLAQKEEQEVVFVPAFTGLGAPYWDASARGAIFGIEANTKREHLVKATLEAIAFQANDLIKAMASDLNSSIKKIKADGGACNSNYLMQFQADIANLEVIIPKNVETTAMGAAFLAGLAVNY", "aa_hexdigest": "38e76e5f49cf0e126bc0b130ac86a101", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02782.22", "name": "FGGY_C", "length": 119, "aa_cov": 0.8939393939393939, "hmm_cov": 1.0, "evalue": 3.5063857955417465e-34, "score": 113.78630828857422, "start": 12, "stop": 129 } ], "seq_stats": { "molecular_weight": 14170.988799999977, "isoelectric_point": 4.910002326965332 }, "id": "GIHJHMOALN_150", "locus": "GCA009939745_00110" }, { "type": "cds", "sequence": "contig_1", "start": 62463, "stop": 62705, "strand": "-", "frame": 2, "gene": null, "product": "FGGY-family carbohydrate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030F9964F" ], "nt": "ATGGTACCTATTCGTGGGGTTGCCGGTGACCAACAAGCAGCCTTGTTTGGTCAGCTCTGCGTGGAACCAGCGATGGTTAAAAACACCTATGGTACAGGTTGTTTTATGTTGATGAACATCGGTAACGAACTGAAATACTCCCAACATAACTTATTAACAACTGTAGCTTGGCAGTTAGAAAACCAAAAACCGGTGTATGCCTTAGAAGGTAGTGTTTTTGTGGCTGGCGCAGCCTTAAAGTGA", "aa": "MVPIRGVAGDQQAALFGQLCVEPAMVKNTYGTGCFMLMNIGNELKYSQHNLLTTVAWQLENQKPVYALEGSVFVAGAALK", "aa_hexdigest": "859321ebd9901156384390060af99b60", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030F9964F", "query_cov": 0.9625, "subject_cov": 0.9625, "identity": 0.5579999999999999, "score": 90.9, "evalue": 1.94e-22, "valid": false, "product": "FGGY-family carbohydrate kinase", "uniref50_id": "UniRef50_UPI0030F9964F" }, "pscc": { "uniref50_id": "UniRef50_UPI0030F9964F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030F9964F" ], "product": "FGGY-family carbohydrate kinase" }, "genes": [], "id": "GIHJHMOALN_151", "locus": "GCA009939745_00111" }, { "type": "cds", "sequence": "contig_1", "start": 63156, "stop": 63245, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGTGCTAAAAATAAGGCACAAATTAAATCAAACAACATTGCTGCTGTAGGGATTACCAACCAACGGGAAACCATTGTGTTATGA", "aa": "MQSAKNKAQIKSNNIAAVGITNQRETIVL", "aa_hexdigest": "e00dcb1327047a07f6c13396c7acb3ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3141.6012, "isoelectric_point": 10.288976860046386 }, "id": "GIHJHMOALN_152", "locus": "GCA009939745_00112" }, { "type": "cds", "sequence": "contig_1", "start": 63264, "stop": 63428, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00370.27" ], "nt": "ATGGATCTAAAACAACAATACATTCTTGCTCTAGATGAAGGAACCAGTTCTTGCCGCACGATTGTGTTTGACAAGGATTTAAACCAAGTAGCGATTGCCCAAAACGAATTTAACCAGTTCTTTCCTAAGAGTGGTTGGGTCGAACAAGATCCCTTAGAAATTTGA", "aa": "MDLKQQYILALDEGTSSCRTIVFDKDLNQVAIAQNEFNQFFPKSGWVEQDPLEI", "aa_hexdigest": "f6d119b44bcbba3c45013df43b23ba03", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920FAT8", "query_cov": 0.9074074074074074, "subject_cov": 0.8909090909090909, "identity": 0.612, "score": 70.5, "evalue": 4.25e-15, "valid": false, "product": "Carbohydrate kinase FGGY N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A920FAT8" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00370.27", "name": "FGGY_N", "length": 48, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 4.894950720242333e-12, "score": 41.419010162353516, "start": 7, "stop": 54 } ], "seq_stats": { "molecular_weight": 6234.928899999999, "isoelectric_point": 4.1646726608276365 }, "id": "GIHJHMOALN_153", "locus": "GCA009939745_00113" }, { "type": "cds", "sequence": "contig_1", "start": 63449, "stop": 63871, "strand": "-", "frame": 3, "gene": null, "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "nt": "GTGGTGGTAACTAATCAGGGCGAACTCCACTTAAACTCAATGGTTTTCATGGTACCAACAATCCATGGTAAAGGGGTAATTGTGTCACCCATGTTAGATGGCAACTTCTTAGTCGGACCCACCGCTTTAGATGGGGTAGACAAGGAAGCTACTCGTTACATTACCAAAGATGCTCCATGTATGTTGACTAAGATTGGCAAACATATGGTGCCAAGTCTAAACATTAATAATGCCTTAATAAGCTTTGCTGGTTCGCGTCCAATTGATAAAGCCACTAATGACTTTATTATTAGAATAGCTCACAATGACCCTGACTTTGTCATTTTAGGGGGTATGAAATCCCCAGGGCTAACTGCAGCACCAGCCATTGCCCGGGAAGCTGTCAGACTTTTAAATTGGAAATTAACTAAAAAGCCGAACTGA", "aa": "MVVTNQGELHLNSMVFMVPTIHGKGVIVSPMLDGNFLVGPTALDGVDKEATRYITKDAPCMLTKIGKHMVPSLNINNALISFAGSRPIDKATNDFIIRIAHNDPDFVILGGMKSPGLTAAPAIAREAVRLLNWKLTKKPN", "aa_hexdigest": "9c41a12d09746eef9ba1da1d184c3d52", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449B2Q2", "query_cov": 0.9, "subject_cov": 0.9264705882352942, "identity": 0.516, "score": 135.0, "evalue": 2.28e-38, "valid": false, "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A449B2Q2" }, "pscc": { "uniref50_id": "UniRef50_A0A449B2Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "product": "Glycerol-3-phosphate dehydrogenase" }, "genes": [], "id": "GIHJHMOALN_154", "locus": "GCA009939745_00114" }, { "type": "cds", "sequence": "contig_1", "start": 64001, "stop": 64114, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGCGAGCAAATGTTTAGCTTTAGCAGCACAGCAAAACAAAGTACAAATTTGCACTAATACTGAGGTTACTAATATTAGTAAGCAAGTTGATGGGACTTATTTAGTTTGA", "aa": "MLASKCLALAAQQNKVQICTNTEVTNISKQVDGTYLV", "aa_hexdigest": "74125aa4a908644cccc537cbd170a1fc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3997.6159, "isoelectric_point": 7.789279747009277 }, "id": "GIHJHMOALN_155", "locus": "GCA009939745_00115" }, { "type": "cds", "sequence": "contig_1", "start": 64127, "stop": 64282, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGCGAACAATTAGAAGTACTAAAGCAAAGAGGCATTGCCAATCAAATTAATCTGGAAGATATTCAAATGCTCTCTAAAGAAGAAACCTTAAAGTTAGAGCCTTACGTTAATCCGGAAATTGTGGCTGGATTAAAAGTCGAGGGTAGCTGA", "aa": "MEREQLEVLKQRGIANQINLEDIQMLSKEETLKLEPYVNPEIVAGLKVEGS", "aa_hexdigest": "5b38d9513bf9171fc49916c66c7c925d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5824.6353, "isoelectric_point": 4.605061149597168 }, "id": "GIHJHMOALN_156", "locus": "GCA009939745_00116" }, { "type": "cds", "sequence": "contig_1", "start": 64355, "stop": 64573, "strand": "-", "frame": 3, "gene": null, "product": "FAD-dependent oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00296496E8" ], "nt": "ATGGAAACAAGAGATGTTTTAATAGTTGGCGGTGGTGTAATAGGCTGTGCCACTGCTTATGAATTGAGCCAGTACAAGCTCAAAGTGACACTGGTAGAAAAGCACCATTATTTGGCACAAGAGACTAGCCATGCCAACAGTGGTGTTATCCACACTGGCATTGATCCCAATCCACACAAGTTAACTGCTAAATACAACATTTTGGGTAAAAAGCTCTGA", "aa": "METRDVLIVGGGVIGCATAYELSQYKLKVTLVEKHHYLAQETSHANSGVIHTGIDPNPHKLTAKYNILGKKL", "aa_hexdigest": "5e84aaeae96c19411d08c02039616fdf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00296496E8", "query_cov": 0.9583333333333334, "subject_cov": 0.92, "identity": 0.522, "score": 76.3, "evalue": 7.58e-17, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_UPI00296496E8" }, "pscc": { "uniref50_id": "UniRef50_UPI00296496E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00296496E8" ], "product": "FAD-dependent oxidoreductase" }, "genes": [], "id": "GIHJHMOALN_157", "locus": "GCA009939745_00117" }, { "type": "cds", "sequence": "contig_1", "start": 64756, "stop": 65019, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGAAAAGGGTTGGTCAAAAAAGGCCTTTTATTTTTTAGTGGCGTGAGTACTATGGCCGTCTTTTTAGTGTCTTGTGGTGCTACTAGAATTTGGGAATCTTCGATTCAACTGTTAGTTTCCAATGATGAAGCTACATTAGCTGACAAATCCTTTTCTGAGATGTCCTATGAAGGGATTAGAAGGTACTTTAGATCTCAAAAACATATTGAATTACCATCACCAAATAGTTCCCTGTTACAAGACGGCAACGGCCTTTGA", "aa": "MFGKGLVKKGLLFFSGVSTMAVFLVSCGATRIWESSIQLLVSNDEATLADKSFSEMSYEGIRRYFRSQKHIELPSPNSSLLQDGNGL", "aa_hexdigest": "7fd589358455c31304d46f75cc35efe1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9593.904499999991, "isoelectric_point": 7.894234275817872 }, "id": "GIHJHMOALN_158", "locus": "GCA009939745_00118" }, { "type": "cds", "sequence": "contig_1", "start": 65089, "stop": 66102, "strand": "+", "frame": 1, "gene": "med", "product": "putative lipo MG040-like protein", "db_xrefs": [ "COG:COG1744", "COG:T", "GO:0005886", "KEGG:K07335", "SO:0001217", "UniRef:UniRef50_P47286", "UniRef:UniRef90_P75062" ], "nt": "GTGATTGTAGCTACTGGATTTAACCAACAAGAAGCACTACAAGCAATTAGCTCTGATGACCGTCGTTATTTAGCTGATAAAAACGACCTTGCTAAAGTTGGTTTCATTTTTGTCGATGGCCAAATTGAAAAGGAATATAACGTGATTAATAAAACGCCACAATTTAGATCAACCCCACTAAACATTTCATCCGTTGCCTTCCGCAGTGATGATGGTTCCTTCTTAACTGGAGTAGCAACTGCAGTTTATTTGAATTTAAATCAAGATTACTTTTTAAAGAAGAATGGAGCAACCAATAATAGTAGCCAAGATTTAACCGTTTCTGGTTTTGTCGGTGTTCCTATTCCTTCAACCCTTTCTTTTTTAAACGGTTTCCGTTTGGGCATTGCTTATTTTAATGAGGTGATTTACACCCATTTAAGTGATGCTGAAACAACATCTGATAACAAGAGTAATTCTTCAAGTGCTAGCAACTCTGTTTTAGTGCAACTTAAACAGATGCAAGGAAACGACAAAAAGATTAAAAAGATTAAATGGATATCTCCTAAACAAGGAAGCGATAATAATTCGAACCTCAGTATCGATGATCACAAGTCTGGTTCTTTCTCCAGCACTGAACCACGAGCAACAACTATCATTAATAATCTTTTAGACAAGGGTGTGAGTGCCATTATTCCTGTTGCCGGACCGCAAGTAAACTTAGCAGTTAACGAGGTGGCACGTCGCAAAGCGCACACTGCTATTATTGGTGTTGATAGTGCGCAAGAATTATTAGATATTAACCAAGATGCACCTGATAAAGATCAGTTAATCAAAGGTAATAAAAAAATTATTCCTTTCTCTTCTATTAAAGCCTTGGATGTTGCCATTGAAAACATGTTGATTGCCATTCAAAAAGGCAGTGATAATAATGGATATAAAGGATTTGGTTATAACAACATTGGTACGGTTGGAACTAGTTCAGTAGGTATTAGTGAGGCTGGTTATGAATTCTTAATTGATCCAGTTTTTTGA", "aa": "MIVATGFNQQEALQAISSDDRRYLADKNDLAKVGFIFVDGQIEKEYNVINKTPQFRSTPLNISSVAFRSDDGSFLTGVATAVYLNLNQDYFLKKNGATNNSSQDLTVSGFVGVPIPSTLSFLNGFRLGIAYFNEVIYTHLSDAETTSDNKSNSSSASNSVLVQLKQMQGNDKKIKKIKWISPKQGSDNNSNLSIDDHKSGSFSSTEPRATTIINNLLDKGVSAIIPVAGPQVNLAVNEVARRKAHTAIIGVDSAQELLDINQDAPDKDQLIKGNKKIIPFSSIKALDVAIENMLIAIQKGSDNNGYKGFGYNNIGTVGTSSVGISEAGYEFLIDPVF", "aa_hexdigest": "9787d68f39a84e05c118fc11d53cf86d", "start_type": "GTG", "rbs_motif": null, "genes": [ "med" ], "truncated": "both", "pseudogene": { "start": 64789, "stop": 66405, "observations": { "mutation": [ 65017, 66100 ], "stop": [ 65017, 66100 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75062", "query_cov": 1.0, "subject_cov": 0.512937595129376, "identity": 0.997, "score": 639.0, "evalue": 5.84e-226, "gene_start": 1, "gene_end": 337, "reference_start": 112, "reference_end": 448, "reference_sequence": "MFGKGLVKKSLLFFSGVSTMAVFLVSCGATRIWESSIQLLVSNDEATLADKSFSEMSYEGIRRYFRSQKHIELPSPNSSLLQDGNGLWKRPGRTLSDRIATFKNIKNDGSDVIVATGFNQQEALQAISSDDRRYLADKNDLAKVGFIFVDGQIEKEYNVINKTPQFRSTPLNISSVAFRSDDGSFLTGVATAVYLNLNQDYFLKKNGATNNSSQDLTVSGFVGVPIPSTLSFLNGFRLGIAYFNEVIYTHLSDAETTSDNKSNSSSASNSVLVQLKQMQGNDKKIKKIKWISPKQGSDNNSNLSIDDHKSGSFSSTEPRATTIINNLLDKGVSAIIPVAGPQVNLAVNEVARRKAHTAIIGVDSAQELLDINQDAPDKDQLIKGNKKIIPFSSIKALDVAIENMLIAIQKGSDNNGYKGFGYNNIGTVGTSSVGISEAGYEFLIDPVFWKTTQSQGKSMATNMTNLKRLSSDDTNTKKALKEVSTHKNGSDKDGIIGKYSNLLTKKSTTVTAVAQKSMTDNNSGTEQKKNLSEVDTKKKEKESKGKTQSNGQDSGQQNGKETNDIISKYSKLLTMTTMNNKVMSSKKQSSDDNSFKKTSENGDWVIKGDELTKKKSTELPAFAKGADYPTFPTEAVSVINGSTALDGKGFKWSFKQI" }, "paralog": false, "identity": 0.9962825278810409, "score": 1038.0, "evalue": 0.0, "description": "Internal stop codon at 65017, 66100. Nonsense mutation around 65017, 66100.", "psc": { "uniref90_id": "UniRef90_P75062", "gene": "Med", "product": "Uncharacterized lipoprotein MG040 homolog", "uniref50_id": "UniRef50_P47286", "cog_id": "COG1744", "cog_category": "T", "kegg_orthology_id": "K07335", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75062", "UniRef:UniRef50_P47286", "COG:COG1744", "COG:T", "KEGG:K07335", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47286", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47286" ], "product": "Uncharacterized lipoprotein MG040" } }, "id": "GIHJHMOALN_159", "locus": "GCA009939745_00119" }, { "type": "cds", "sequence": "contig_1", "start": 66130, "stop": 66567, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCACAAATATGACTAATTTAAAACGTTTATCATCTGATGATACAAACACTAAGAAAGCATTAAAAGAGGTAAGCACCCATAAAAATGGTAGTGATAAAGATGGGATTATTGGTAAATATTCCAATTTATTAACTAAAAAAAGCACAACAGTAACTGCTGTTGCTCAAAAATCTATGACAGACAATAATAGTGGAACTGAGCAAAAAAAGAATCTTTCTGAAGTAGATACTAAGAAGAAAGAGAAAGAAAGTAAAGGTAAAACTCAAAGTAATGGTCAGGACAGTGGCCAACAAAACGGAAAAGAAACAAACGATATTATTTCTAAGTATTCTAAGCTGTTAACTATGACAACTATGAACAATAAAGTAATGTCATCTAAAAAACAAAGTAGCGATGATAACAGTTTTAAAAAGACTTCAGAAAACGGAGACTGA", "aa": "MATNMTNLKRLSSDDTNTKKALKEVSTHKNGSDKDGIIGKYSNLLTKKSTTVTAVAQKSMTDNNSGTEQKKNLSEVDTKKKEKESKGKTQSNGQDSGQQNGKETNDIISKYSKLLTMTTMNNKVMSSKKQSSDDNSFKKTSENGD", "aa_hexdigest": "3e707f1c1aff50d4d726a2f89400c525", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15895.454799999989, "isoelectric_point": 9.602001762390138 }, "id": "GIHJHMOALN_160", "locus": "GCA009939745_00120" }, { "type": "cds", "sequence": "contig_1", "start": 66747, "stop": 67013, "strand": "+", "frame": 3, "gene": "ptsH", "product": "phosphocarrier protein HPr", "db_xrefs": [ "BlastRules:WP_000411080", "BlastRules:WP_010874410", "COG:COG1925", "COG:TG", "GO:0005737", "GO:0009401", "RefSeq:WP_010874410.1", "SO:0001217", "UniParc:UPI00001328F5", "UniRef:UniRef100_P75061", "UniRef:UniRef50_P75061", "UniRef:UniRef90_P75061" ], "nt": "ATGAAGAAGATTCAAGTAGTCGTTAAAGACCCTGTGGGCATTCACGCCCGTCCTGCTAGTATTATTGCTGGTGAGGCTAATAAATTTAAATCGGAATTAAAGCTAGTATCACCAAGCGGCGTTGAAGGTAACATCAAGTCGATCATAAACTTAATGTCTTTAGGCATTAAGCAAAACGACCACATTACCATCAAGGCAGAAGGAACTGATGAAGAAGAGGCACTAAATGCTATTAAAGCAGTTTTAGAAAAACACCAAGTTATTTAA", "aa": "MKKIQVVVKDPVGIHARPASIIAGEANKFKSELKLVSPSGVEGNIKSIINLMSLGIKQNDHITIKAEGTDEEEALNAIKAVLEKHQVI", "aa_hexdigest": "ea7f7abc14674640ebb74226629ff732", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI00001328F5", "ncbi_nrp_id": "WP_010874410.1", "uniref100_id": "UniRef100_P75061", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001328F5", "RefSeq:WP_010874410.1", "UniRef:UniRef100_P75061" ] }, "ips": { "uniref100_id": "UniRef100_P75061", "uniref90_id": "UniRef90_P75061" }, "psc": { "uniref90_id": "UniRef90_P75061", "gene": "ptsH", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_P75061", "cog_id": "COG1925", "cog_category": "TG", "go_ids": [ "GO:0005737", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75061" ], "product": "Phosphocarrier protein HPr" }, "expert": [ { "type": "expert_proteins", "source": "BlastRules", "rank": 80, "id": "WP_010874410", "gene": "ptsH", "product": "phosphocarrier protein HPr", "query_cov": 1.0, "subject_cov": 1.0, "identity": 1.0, "score": 168.0, "evalue": 1.27e-56, "db_xrefs": [ "BlastRules:WP_000411080", "BlastRules:WP_010874410" ] } ], "genes": [ "ptsH" ], "id": "GIHJHMOALN_161", "locus": "GCA009939745_00121" }, { "type": "cds", "sequence": "contig_1", "start": 67090, "stop": 67215, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTACTACGACGACGAGGATGAATATGACACTTCTAATTCTGGTGGTGTCGTAGATTTAGCATTTTTAGCACTAATAGTAGTTTTTCTATTTACCGCTTTTATTTCTTGGTTTGGGGCATGA", "aa": "MLYYDDEDEYDTSNSGGVVDLAFLALIVVFLFTAFISWFGA", "aa_hexdigest": "722943f8e320b6775e88410304ac02b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4616.109799999998, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_162", "locus": "GCA009939745_00122" }, { "type": "cds", "sequence": "contig_1", "start": 67942, "stop": 68166, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI000B1E1D42", "UniRef:UniRef100_A0AB38W877", "UniRef:UniRef50_A0AB38W877", "UniRef:UniRef90_A0AB38W877" ], "nt": "GTGTTTCTCAAATATTTCTCTAAGAATGAGATTTTTATTCTTTGGTATTTCCACGTTATTTATCGAAATAATCTCTTTTTATTTCTAGAAAATTACTTTGATTTTTGTCTAAGGATTCTCAACTTAGTCCCTAAATTTTCTCCGCTTTTTAGCGAAGAATATTTCAATTTATTCTTTGATTTTTATCTAAAAACTATCCCCTTAGTTACTTTAAATTTCTGTTAA", "aa": "MFLKYFSKNEIFILWYFHVIYRNNLFLFLENYFDFCLRILNLVPKFSPLFSEEYFNLFFDFYLKTIPLVTLNFC", "aa_hexdigest": "e953e932fffa4f1103799e920d23464a", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000B1E1D42", "uniref100_id": "UniRef100_A0AB38W877", "db_xrefs": [ "SO:0001217", "UniParc:UPI000B1E1D42", "UniRef:UniRef100_A0AB38W877" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W877", "uniref90_id": "UniRef90_A0AB38W877" }, "psc": { "uniref90_id": "UniRef90_A0AB38W877", "uniref50_id": "UniRef50_A0AB38W877" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W877", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W877" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9324.917199999989, "isoelectric_point": 6.527128410339355 }, "id": "GIHJHMOALN_163", "locus": "GCA009939745_00123" }, { "type": "cds", "sequence": "contig_1", "start": 68539, "stop": 68769, "strand": "+", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_054", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75060", "UniRef:UniRef50_UPI001FCEF156", "UniRef:UniRef90_P75060", "UniRef:UniRef90_UPI001FCEF156" ], "nt": "ATGAAGATTAAATATTTCTTTATTCCCTTGTTTTCTTCAGTTATATTATTTAGTGCCTGTTCTTCTATTCAGTCTGATCTTAGGAATCTTATTAAAGAAACAACTGGCAAAGATTTTGACGTATCTAAATTAATTAAAACCTCTGAAGGAAGAAAAAACTTAATAAATTCTCTCAAAAAGTCTTACGAATCTAAGCCCGATGAAACAGCTAGCTTATTACTAATTGCTTGA", "aa": "MKIKYFFIPLFSSVILFSACSSIQSDLRNLIKETTGKDFDVSKLIKTSEGRKNLINSLKKSYESKPDETASLLLIA", "aa_hexdigest": "2d543206ab483ba012141d764e9c031f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCEF156", "query_cov": 0.9473684210526315, "subject_cov": 0.9863013698630136, "identity": 1.0, "score": 137.0, "evalue": 4.77e-41, "valid": true, "uniref50_id": "UniRef50_UPI001FCEF156" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCEF156", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCEF156" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 68521, "stop": 68910, "observations": { "mutation": [ 68767 ], "stop": [ 68767 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75060", "query_cov": 1.0, "subject_cov": 0.5891472868217055, "identity": 0.987, "score": 142.0, "evalue": 3.79e-42, "gene_start": 1, "gene_end": 76, "reference_start": 7, "reference_end": 82, "reference_sequence": "RYLSNLMKIKYFFIPLFSSAILFSACSSIQSDLRNLIKETTGKDFDVSKLIKTSEGRKNLINSLKKSYESKPDETASLLLIAWKQSAEMGEIGFDDIKEGGIYTRDNDPFKLEQKVEYFNMEYKILVMF" }, "paralog": false, "identity": 0.9844961240310077, "score": 249.0, "evalue": 1.02e-86, "description": "Internal stop codon at 68767. Nonsense mutation around 68767.", "psc": { "uniref90_id": "UniRef90_P75060", "product": "Uncharacterized lipoprotein MPN_054", "uniref50_id": "UniRef50_P75060", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75060", "UniRef:UniRef50_P75060", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75060", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75060" ], "product": "Uncharacterized lipoprotein MPN_054" } }, "id": "GIHJHMOALN_164", "locus": "GCA009939745_00124" }, { "type": "cds", "sequence": "contig_1", "start": 68785, "stop": 68910, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGAAATAGGTTTTGATGATATTAAAGAAGGAGGAATTTATACCAGAGACAATGATCCTTTCAAATTGGAACAAAAGGTTGAGTACTTCAATATGGAATACAAAATTTTAGTGATGTTTTAA", "aa": "MGEIGFDDIKEGGIYTRDNDPFKLEQKVEYFNMEYKILVMF", "aa_hexdigest": "43d7734811b693ec99330b72d074c09f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4924.5788999999995, "isoelectric_point": 4.399986076354981 }, "id": "GIHJHMOALN_165", "locus": "GCA009939745_00125" }, { "type": "cds", "sequence": "contig_1", "start": 70316, "stop": 71203, "strand": "+", "frame": 2, "gene": "potA", "product": "Spermidine/putrescine import ATP-binding protein PotA", "db_xrefs": [ "COG:COG3839", "COG:G", "EC:7.6.2.11", "GO:0005524", "GO:0005886", "GO:0015417", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75059", "UniRef:UniRef90_P75059" ], "nt": "TTGAACGAACGTTTTTTAATTGAAATCGAAGGTTTAAACAAAACTTTCGATGATGGTTTTGTATCAGTACGTGATATTAACCTCAAAATTAAGAAGGGCGAATTTATTACGATTCTCGGTCCTTCTGGTTGTGGCAAAACAACTACTCTTAGGTTGTTAGCAGGTTTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTCTTGACATTAAAGACTTGCCCATTCATAAGCGTCCCTTTGCTACTGTCTTCCAAGACTATGCCTTGTTTTCACACTTAACTGTGTACAAGAACATTGCTTATGGCTTGAAGGCAATGTACACAAAGCTGGATCCGATTGATAAGCTAGTGGAACAATACCACCAAAGCTTACTGGACAAGCAACACCGCTTGCACAAGAGGATTGAACGCTTAGAAAAAAGTAATGCTAATCCACAACTGTTAGAACAACTTAAACAAACGGTTGTCGTTCAACAAAAACAGTTTAAACAACAAACAGAGACTTTTAAACAAAAGGAAAATGCTCGACGTGATGCCATTCAACAACGCTTGGTGCAACTTACCAAGGAATGGGAAAGCTTATCAAAGCAGAAGTTGCAACAATTAGAAGCGGAGAAAAAGTTGTTAGATAAAAAGTTTGAACAAACTGAGCGCAAATACCAAAAGGATGCTTGGATGGCAACCCACAGTGAAATGCGTTTAAAGCAGTTCAAACAAGAGGTGTTAGCTTTAAAACAACTAATTAAAACTAAGTTCAAACAAAATGAACCAGTTGACAAGTTGCAGTTAAAGCTGCAAACCTTAAAGCAAAAGTATGCTGCTAAAAGACAAATTGATAAGGAATACGATAAGTTGGTCTTAGCCTACAACAAGAAGGACTATTGA", "aa": "MNERFLIEIEGLNKTFDDGFVSVRDINLKIKKGEFITILGPSGCGKTTTLRLLAGFEDPTYGKIKVNGLDIKDLPIHKRPFATVFQDYALFSHLTVYKNIAYGLKAMYTKLDPIDKLVEQYHQSLLDKQHRLHKRIERLEKSNANPQLLEQLKQTVVVQQKQFKQQTETFKQKENARRDAIQQRLVQLTKEWESLSKQKLQQLEAEKKLLDKKFEQTERKYQKDAWMATHSEMRLKQFKQEVLALKQLIKTKFKQNEPVDKLQLKLQTLKQKYAAKRQIDKEYDKLVLAYNKKDY", "aa_hexdigest": "3c064fba9f648766d04a231e95ea2d8b", "start_type": "TTG", "rbs_motif": "AAAAA", "genes": [ "potA" ], "truncated": "3-prime", "pseudogene": { "start": 70316, "stop": 71506, "observations": { "mutation": [ 71201 ], "stop": [ 71201 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75059", "query_cov": 1.0, "subject_cov": 0.5267857142857143, "identity": 1.0, "score": 550.0, "evalue": 2.35e-193, "gene_start": 1, "gene_end": 295, "reference_start": 1, "reference_end": 295, "reference_sequence": "MNERFLIEIEGLNKTFDDGFVSVRDINLKIKKGEFITILGPSGCGKTTTLRLLAGFEDPTYGKIKVNGLDIKDLPIHKRPFATVFQDYALFSHLTVYKNIAYGLKAMYTKLDPIDKLVEQYHQSLLDKQHRLHKRIERLEKSNANPQLLEQLKQTVVVQQKQFKQQTETFKQKENARRDAIQQRLVQLTKEWESLSKQKLQQLEAEKKLLDKKFEQTERKYQKDAWMATHSEMRLKQFKQEVLALKQLIKTKFKQNEPVDKLQLKLQTLKQKYAAKRQIDKEYDKLVLAYNKKDYWTSYWETYSLQQQEAFEKRYLSRKLTKQEQHQKVCAVIELVGLKGNEDKLPEELSGGMKQRVALARSLVIEPDILLLDEPLSALDAKVRKNLQKELQKIHQQSGLTFILVTHDQEEALVLSNRIVVMNEGNILQVGSPADIYDSPKTEWIANFIGQANIFKGTYLGDLKIKLHSGEVIKTDVDNNYVVGKEYKILIRPEDFDIVPKNKGFFNVRVIDKTYKGLLWKITTKLVDDTIVDLESVNDIEVDKTFGVTFDPIDVHLMEV" }, "paralog": false, "identity": 0.9949494949494949, "score": 775.0, "evalue": 5.55e-284, "description": "Internal stop codon at 71201. Nonsense mutation around 71201.", "psc": { "uniref90_id": "UniRef90_P75059", "gene": "potA", "product": "Spermidine/putrescine import ATP-binding protein PotA", "ec_ids": [ "7.6.2.11" ], "uniref50_id": "UniRef50_P75059", "cog_id": "COG3839", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0015417", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75059", "EC:7.6.2.11", "UniRef:UniRef50_P75059", "COG:COG3839", "COG:G", "GO:0005886", "GO:0015417", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75059", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75059" ], "product": "Spermidine/putrescine import ATP-binding protein PotA" } }, "id": "GIHJHMOALN_166", "locus": "GCA009939745_00126" }, { "type": "cds", "sequence": "contig_1", "start": 71300, "stop": 71875, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F197FDC", "UniRef:UniRef90_UPI001F197FDC" ], "nt": "GTGTGTGCTGTAATTGAGTTAGTTGGTTTAAAGGGTAACGAGGACAAACTCCCAGAAGAATTGTCTGGGGGAATGAAGCAACGGGTAGCCTTAGCGCGTTCCTTGGTAATTGAACCAGACATTCTCTTATTGGATGAACCCTTGTCTGCTTTAGATGCTAAGGTACGTAAAAACTTGCAAAAGGAATTGCAAAAGATTCACCAACAAAGTGGTTTAACCTTTATCTTAGTTACTCACGACCAGGAAGAAGCCTTGGTCTTGTCCAACCGCATTGTGGTAATGAATGAGGGTAACATTCTCCAAGTTGGTAGTCCGGCTGATATTTACGATTCACCCAAAACCGAGTGGATTGCTAACTTCATTGGTCAAGCCAATATCTTTAAGGGTACTTACCTAGGTGACCTTAAGATTAAACTGCACAGCGGTGAAGTGATTAAAACTGATGTGGACAATAACTATGTGGTTGGTAAGGAATACAAAATCCTAATTCGTCCCGAAGACTTTGACATTGTGCCCAAAAACAAAGGCTTCTTTAATGTTCGGGTAATTGATAAAACCTACAAAGGATTACTCTGA", "aa": "MCAVIELVGLKGNEDKLPEELSGGMKQRVALARSLVIEPDILLLDEPLSALDAKVRKNLQKELQKIHQQSGLTFILVTHDQEEALVLSNRIVVMNEGNILQVGSPADIYDSPKTEWIANFIGQANIFKGTYLGDLKIKLHSGEVIKTDVDNNYVVGKEYKILIRPEDFDIVPKNKGFFNVRVIDKTYKGLL", "aa_hexdigest": "c9980fc5d079bbf83c983fbc1d91f765", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "both", "pseudogene": { "start": 71171, "stop": 71998, "observations": { "mutation": [ 71201, 71873 ], "stop": [ 71201, 71873 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F197FDC", "query_cov": 0.9842931937172775, "subject_cov": 0.6836363636363636, "identity": 0.883, "score": 337.0, "evalue": 4.74e-115, "gene_start": 4, "gene_end": 191, "reference_start": 47, "reference_end": 234, "reference_sequence": "MVVAYNKKDYWTSYWETYTLQQKEAFEKRYLSRKLTKAEQNKKVSDVIEMVGLKGKEDRLPDELSGGMKQRVALARSLVVEPEILLLDEPLSALDAKVRKNLQKELQQIHKKSGLTFILVTHDQEEALVLSDRIVVMNEGNILQVGNPVDIYDSPKTEWIANFIGQANIFKGTYLGEKKIQLQSGEIIQTDVDNNYVVGKQYKILIRPEDFDLVPENKGFFNVRVIDKNYKGLLWKITTQLKDNTIVDLESVNEVDVNKTFGVLFDPIDVHLMEV" }, "paralog": false, "identity": 0.850909090909091, "score": 473.0, "evalue": 3.95e-171, "description": "Internal stop codon at 71201, 71873. Nonsense mutation around 71201, 71873.", "psc": { "uniref90_id": "UniRef90_UPI001F197FDC", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI001F197FDC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F197FDC", "UniRef:UniRef50_UPI001F197FDC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F197FDC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F197FDC" ], "product": "ABC transporter ATP-binding protein" } }, "id": "GIHJHMOALN_167", "locus": "GCA009939745_00127" }, { "type": "cds", "sequence": "contig_1", "start": 72000, "stop": 72176, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTATCGAAAAAATACCTCTTAGCTGTACCATTCTTTGTTTTAATGGTCATCTTCTTTGTAGTACCAATGGCATGGATTATTGTTAGTGGACTCCAAAATGAAAACGGTGCAAGTATAACGGAAAAGTACCAACCCTTAGTTGGTGGTTATTCTTTCTTTCAGAGTTTTTGA", "aa": "MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSF", "aa_hexdigest": "175b6ae90ee0fa8116a19eac8dbc2b8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6595.827299999999, "isoelectric_point": 9.224861717224119 }, "id": "GIHJHMOALN_168", "locus": "GCA009939745_00128" }, { "type": "cds", "sequence": "contig_1", "start": 72198, "stop": 72860, "strand": "+", "frame": 3, "gene": "potB", "product": "Spermidine/putrescine transport system permease PotB-like protein", "db_xrefs": [ "COG:COG1176", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47289", "UniRef:UniRef90_P75058" ], "nt": "GTGACAGTACTAGTAGCCTTACTGGTTGCCTTTCCCTTTTGTTACTTTTTATCACAAAGTAAAAACAAGGTATTCCGCAGTTTTGTGATTGCCTTAGCTACTGCTCCCATATGGAGTAGTTTTCTCATTAAGTTAATTGGGTTAAAAACACTGTTGGACTTAGTTTTGGGGTTAGCGTTGAACCGGGTGGGTGATAATAACCTCACCTTTGGTAGTGGTTATACCTTGATTGGGATGATCTACCTGTTCACCCCCTTCATGTTTTTACCACTTTACAACAACTTTTGTATCTTACCGAAAAACTTAATCTTAGCTAGTCAAGATTTGGGTTACAACTGGATTACGAGTTTTATTAAGGTAGTTATTCCCTTTTCTAAAACTGCCATTCTGTCAGGGATTGCGTTAACTTTCTTTCCGTCTTTAACCTCGGTAGCGATTGCCCAGTTCTTGGACAACTCGAATCAAAATAACACCTTAGGTAACTATGTCTTTACTTTAGGTAACAATGGTTATGACAGTGCGATTGAGCGCGGCCGTGCTTCTGGTGCCATTATTATTGCGGCTTTAATTACCTTTGCCTTTTACTTTGTGGTGATCTTTGCTCCAAGAATAGTGCGCTTAATCCAGACTAAGTGTTTGAAATACAGGAGGGTTAATGTTTAA", "aa": "MTVLVALLVAFPFCYFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVLGLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNNFCILPKNLILASQDLGYNWITSFIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSAIERGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLKYRRVNV", "aa_hexdigest": "74deb28a5e8186ae46d2c289a5eb4c4c", "start_type": "GTG", "rbs_motif": null, "genes": [ "potB" ], "truncated": "5-prime", "pseudogene": { "start": 72000, "stop": 72860, "observations": { "mutation": [ 72174, 72186 ], "stop": [ 72174, 72186 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75058", "query_cov": 0.9590909090909091, "subject_cov": 0.7377622377622378, "identity": 1.0, "score": 404.0, "evalue": 5.11e-141, "gene_start": 10, "gene_end": 220, "reference_start": 76, "reference_end": 286, "reference_sequence": "MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSFWTSLWTATVTVLVALLVAFPFCYFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVLGLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNNFCILPKNLILASQDLGYNWITSFIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSAIERGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLKYRRVNV" }, "paralog": false, "identity": 0.9370629370629371, "score": 520.0, "evalue": 6.89e-189, "description": "Internal stop codon at 72174, 72186. Nonsense mutation around 72174, 72186.", "psc": { "uniref90_id": "UniRef90_P75058", "gene": "potB", "product": "Spermidine/putrescine transport system permease protein PotB homolog", "uniref50_id": "UniRef50_P47289", "cog_id": "COG1176", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75058", "UniRef:UniRef50_P47289", "COG:COG1176", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47289" ], "product": "Spermidine/putrescine transport system permease protein PotB homolog" } }, "id": "GIHJHMOALN_169", "locus": "GCA009939745_00129" }, { "type": "cds", "sequence": "contig_1", "start": 72921, "stop": 73130, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATCGGGATCGAGACAATGACCGTAATTAAGCCAATAATGACAGAGTTGAGTAAAGGGATGGCAAAGTCCGCTTCCCCTAATCTTAGATATGCTGACTTGGCATCCGGGTTAGGGTTTAAAACATTACCAAAGTCTAAGACAATGTTACCCCTAGTCGAAGAACCGTTAAAACTAACTAAGACAACTATTATTAAAGGGAGGTAG", "aa": "MAIGIETMTVIKPIMTELSKGMAKSASPNLRYADLASGLGFKTLPKSKTMLPLVEEPLKLTKTTIIKGR", "aa_hexdigest": "2b3c3c00e5a33e4d820a425e9bb38ff8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7461.976099999998, "isoelectric_point": 9.996741676330569 }, "id": "GIHJHMOALN_170", "locus": "GCA009939745_00130" }, { "type": "cds", "sequence": "contig_1", "start": 73132, "stop": 73587, "strand": "+", "frame": 1, "gene": "potC", "product": "Spermidine/putrescine transport system permease PotC-like protein", "db_xrefs": [ "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47290", "UniRef:UniRef90_P75057" ], "nt": "ATGACTGCCTTTGCTTTATTGCGATCCAGGCAATGGCTCAATAAAACAGTCTTTGGGATTGCTAACTTTTCTCTAGCAACTCCCGATATTATTACCGGGATATCGCTAGTGTTGTTATTTGCCAACACCTGGTTGAGCTTTAACCAACAGTTAGGTTTCTTTACTATTATTAGTTCCCACATCTCCTTTTCGGTGCCTTATGCCTTGGTGTTAATTTACCCAAAGATGCAAAAGTTAAACCGTAACTTAATCCTAGCGAGTCAGGATTTGGGTTACTCACCCATTGCTACCTTCTTTCACATTACCTTACCTTACTTACTACCGAGTATCTTGTCAGCCATTCTAGTAGTGTTTGCCACGAGCTTTGATGACTATGTGATTACTTCCTTAGTTCAAGGTTCAGTTAAAACAGTCGCCAGTGAGCTCTATTCCTTTAGAAAGGGGATTAAGGCCTGA", "aa": "MTAFALLRSRQWLNKTVFGIANFSLATPDIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPYALVLIYPKMQKLNRNLILASQDLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRKGIKA", "aa_hexdigest": "f2806938ae3e849e0fff7f1ce6a673f7", "start_type": "ATG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "both", "pseudogene": { "start": 72853, "stop": 73713, "observations": { "mutation": [ 72880, 73585, 73699 ], "stop": [ 72880, 73585, 73699 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75057", "query_cov": 1.0, "subject_cov": 0.527972027972028, "identity": 1.0, "score": 283.0, "evalue": 1.89e-94, "gene_start": 1, "gene_end": 151, "reference_start": 94, "reference_end": 244, "reference_sequence": "MFKMKSCKLWLRGSFFVIVLVLIYLPLIIVVLVSFNGSSTRGNIVLDFGNVLNPNPDAKSAYLRLGEADFAIPLLNSVIIGLITVIVSIPIAIMTAFALLRSRQWLNKTVFGIANFSLATPDIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPYALVLIYPKMQKLNRNLILASQDLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRKGIKAWAIAFGTILILVSILAVLLVTLHKYLRFKHKEMLRVKQWKNS" }, "paralog": false, "identity": 0.986013986013986, "score": 533.0, "evalue": 3.23e-195, "description": "Internal stop codon at 72880, 73585, 73699. Nonsense mutation around 72880, 73585, 73699.", "psc": { "uniref90_id": "UniRef90_P75057", "gene": "potC", "product": "Spermidine/putrescine transport system permease protein PotC homolog", "uniref50_id": "UniRef50_P47290", "cog_id": "COG1177", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75057", "UniRef:UniRef50_P47290", "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47290" ], "product": "Spermidine/putrescine transport system permease protein PotC homolog" } }, "id": "GIHJHMOALN_171", "locus": "GCA009939745_00131" }, { "type": "cds", "sequence": "contig_1", "start": 73698, "stop": 73982, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAGTTAAAGTATTGGTTTAGTCTAATGGGACTAACCATGTCAGTTGGGTTAACTGCTTGTAGTTCCAACCAGTTTGTGTTTGCTAACTTTGAGTCGTATGTGTCGCCCTTACTTTTAGAAAGGGCAGAAGCCAAGCAACCAATGACCTTTCTTACTTATCCTACTAACGAAAAGTTAATTAACGGATTTGCTAACAATACCTATACGGTAGCAGTGGCCTCGAGTTATGCTGTGAGTGAACTGCAGCAACAAGGCCACTTACTACCAATTGATTGA", "aa": "MKKQLKYWFSLMGLTMSVGLTACSSNQFVFANFESYVSPLLLERAEAKQPMTFLTYPTNEKLINGFANNTYTVAVASSYAVSELQQQGHLLPID", "aa_hexdigest": "6dc6f5b0027591970040725680a8f495", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10535.000199999991, "isoelectric_point": 6.545373725891113 }, "id": "GIHJHMOALN_172", "locus": "GCA009939745_00132" }, { "type": "cds", "sequence": "contig_1", "start": 74292, "stop": 75152, "strand": "+", "frame": 3, "gene": "potD", "product": "putative lipo MG045-like protein", "db_xrefs": [ "COG:COG0687", "COG:E", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75056", "UniRef:UniRef90_P75056" ], "nt": "GTGCACCATGAGGTTAAGAATACCACGGTAGATGTTAATCCTACAGGTAGCACCTTAAACTACTTTGGCAACGTTTACGAAAGTTTTGCAAATTTAGGTCTAAAACGTGATAACCTCAACACGCTCTTTGTCAATTCGGACTCCAACATTATTATTAATGAATTAGCTAATGGCAGAAGACAAGGTGGCATTGTTTACAATGGTGATGCCGTATATGCCGCTTTGGGCGGTGATCTCCGCGATGAAATTAATGAGAATAACTTGCCCAACGGCGATAACTTCCACATTGTGCAACCGAAACACTCGCCTGTGGCCCTAGACTTTCTCATTATTAACCAGCAACAAACTCACTTTAGAGATGCTGCACACCAGTTAATTTACCAGTTGGCCTTAGAAGGCGCTGATCAAACAGCAGAAGAATTATTAAAAACTGATGAAGAGAAAGGTACCAGTGATGAGGACTATTACACTTATGGTGCAATGCAGAATTTTAGTTACGTTAACTATGTTTCGCCTTTAAAGAATATTTCTGATGAAACCACTGGGATTGTGTTTAAGGAAAACAAACAAGCTGATACTAAGCGAGTTGTCAAACAACAAAGTCAATCAGAACAACAATCTGAATCAGCAGAAAAGGAAGAAACGGAACAAGATGATTTCTACACTGCTACTCTCAAAAGTTTGTTGAAAGCGGACAGTTTAGATGACAAAGCTAAGAAGTTAGTAGACACAATTAAAAAGACTTACAAGATCAAAAAAGCCGACAATATTAATTGGGCTAACTTAATCGAGAAACCAATTACCCCATTACAAAGGAGTAATTTAACATTATCGTGGCTTGACTTTAAGCAACGCTTTTGA", "aa": "MHHEVKNTTVDVNPTGSTLNYFGNVYESFANLGLKRDNLNTLFVNSDSNIIINELANGRRQGGIVYNGDAVYAALGGDLRDEINENNLPNGDNFHIVQPKHSPVALDFLIINQQQTHFRDAAHQLIYQLALEGADQTAEELLKTDEEKGTSDEDYYTYGAMQNFSYVNYVSPLKNISDETTGIVFKENKQADTKRVVKQQSQSEQQSESAEKEETEQDDFYTATLKSLLKADSLDDKAKKLVDTIKKTYKIKKADNINWANLIEKPITPLQRSNLTLSWLDFKQRF", "aa_hexdigest": "394d4ce4bd64700c3123ea2c05a6d377", "start_type": "GTG", "rbs_motif": null, "genes": [ "potD" ], "truncated": "5-prime", "pseudogene": { "start": 73992, "stop": 75152, "observations": { "mutation": [ 74187 ], "stop": [ 74187 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75056", "query_cov": 1.0, "subject_cov": 0.5896907216494846, "identity": 0.972, "score": 531.0, "evalue": 3.44e-187, "gene_start": 1, "gene_end": 286, "reference_start": 199, "reference_end": 484, "reference_sequence": "MKKQLKYWFSLMGLTMSVGLTACSSNQFVFANFESYVSPLLLERAEAKQPMTFLTYPTNEKLINGFANNTYTVAVASSYAVSELQQQGHLLPIDWAKFNLKKTQNGSNQATIQNKEDAKELFTKEIGDISGELLNWGVPYFLQDLVFVYRGEKIQELEGQDVTWSTIIKAIVNHKDRFNNNRLALIDDARTIFSLANVVHHEVKNTTVDVNPTGSTLNYFGNVYESFANLGLKRDNLNTLFVNSDSNIIINELANGRRQGGIVYNGDAVYAALGGDLRDEINENNLPNGDNFHIVQPKHSPVALDFLIINQQQTHFRDAAHQLIYQLALEGADQTAEELLKTDEEKGTSDEDYYTYGAMQNFSYVNYVSPLKNISDETTGIVFKENKQADTKQVVKQQSQSEQQSESAEKEETEQDDFYTATLKSLLKADSLDDKAKKLVDTIKKTYKIKKADNINWANLIEKPITPLQRSNLTLSWLDFKQRFW" }, "paralog": false, "identity": 0.9792746113989638, "score": 751.0, "evalue": 4.71e-276, "description": "Internal stop codon at 74187. Nonsense mutation around 74187.", "psc": { "uniref90_id": "UniRef90_P75056", "gene": "PotD", "product": "Uncharacterized lipoprotein MG045 homolog", "uniref50_id": "UniRef50_P75056", "cog_id": "COG0687", "cog_category": "E", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75056", "UniRef:UniRef50_P75056", "COG:COG0687", "COG:E", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75056" ], "product": "Uncharacterized lipoprotein MG045 homolog" } }, "id": "GIHJHMOALN_173", "locus": "GCA009939745_00133" }, { "type": "cds", "sequence": "contig_1", "start": 75155, "stop": 75769, "strand": "+", "frame": 2, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711", "SO:0001217", "UniRef:UniRef50_P75055", "UniRef:UniRef90_P75055" ], "nt": "ATGGAACAACCTTTGTGTATCTTGGGAATTGAAACCACTTGTGATGACACTAGCATAGGGGTAATCACTGAGAGTAAGGTACAAGCACACATTGTTTTATCATCCGCCAAATTACACGCCCAAACTGGTGGCGTAGTGCCCGAAGTTGCAGCTAGGAGTCACGAACAAAACTTGCTTAAAGCACTGCAACAATCAGGGGTTGTTTTAGAACAAATTACTCACATTGCTTATGCCGCTAATCCGGGATTACCCGGTTGTCTGCATGTGGGAGCTACCTTTGCGCGCAGTTTAAGCTTCTTATTGGACAAACCCTTATTGCCCATCAACCACCTTTATGCCCACATCTTCTCGGCCTTAATTGACCAAGATATTAATCAGTTAAAACTCCCCGCTTTAGGCTTAGTGGTGTCCGGGGGGCACACTGCCATTTACCTAATTAAGTCCTTGTTTGACTTGGAGTTAATTGCTGAGACTAGCGATGATGCGATTGGCGAAGTTTATGATAAGGTGGGCCGCGCTATGGGTTTTCCTTATCCAGCTGGACCCCAGTTAGATAGTTTGTTTCAACCGGAGTTAGTTAAGTCACATTATTTCTTTCGTCCTTCCACGAAGTGA", "aa": "MEQPLCILGIETTCDDTSIGVITESKVQAHIVLSSAKLHAQTGGVVPEVAARSHEQNLLKALQQSGVVLEQITHIAYAANPGLPGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSALIDQDINQLKLPALGLVVSGGHTAIYLIKSLFDLELIAETSDDAIGEVYDKVGRAMGFPYPAGPQLDSLFQPELVKSHYFFRPSTK", "aa_hexdigest": "4be1fae36d08950b16846e51b5ffc889", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 75155, "stop": 76072, "observations": { "mutation": [ 75767 ], "stop": [ 75767 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75055", "query_cov": 1.0, "subject_cov": 0.6394984326018809, "identity": 1.0, "score": 407.0, "evalue": 3.64e-142, "gene_start": 1, "gene_end": 204, "reference_start": 1, "reference_end": 204, "reference_sequence": "MEQPLCILGIETTCDDTSIGVITESKVQAHIVLSSAKLHAQTGGVVPEVAARSHEQNLLKALQQSGVVLEQITHIAYAANPGLPGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSALIDQDINQLKLPALGLVVSGGHTAIYLIKSLFDLELIAETSDDAIGEVYDKVGRAMGFPYPAGPQLDSLFQPELVKSHYFFRPSTKWTKFSYSGLKSQCFTKIKQLRERKGFNPQTHDWNEFASNFQATIIDHYINHVKDAIQQHQPQMLLLGGGVSANKYLREQVTQLQLPYLIAPLKYTSDNGAMIGFYANLLINGKNN" }, "paralog": false, "identity": 0.9967213114754099, "score": 608.0, "evalue": 1.73e-223, "description": "Internal stop codon at 75767. Nonsense mutation around 75767.", "psc": { "uniref90_id": "UniRef90_P75055", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P75055", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75055", "EC:2.3.1.234", "UniRef:UniRef50_P75055", "COG:COG0533", "COG:J", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ] }, "pscc": { "uniref50_id": "UniRef50_P75055", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75055" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "GIHJHMOALN_174", "locus": "GCA009939745_00134" }, { "type": "cds", "sequence": "contig_1", "start": 75791, "stop": 76114, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGTCACAGTGTTTTACCAAGATTAAACAGTTAAGGGAAAGGAAAGGCTTTAATCCCCAAACCCACGATTGGAATGAGTTTGCTTCTAACTTCCAGGCCACCATTATTGATCACTACATCAATCATGTCAAGGATGCCATTCAGCAACACCAACCGCAAATGTTGCTGTTAGGTGGTGGGGTAAGTGCTAATAAATACTTAAGAGAACAGGTAACACAGTTACAGCTGCCTTACTTAATTGCGCCGCTCAAGTACACGAGCGATAACGGGGCGATGATTGGTTTTTATGCTAATCTTTTAATTAATGGCAAAAACAATTAA", "aa": "MKSQCFTKIKQLRERKGFNPQTHDWNEFASNFQATIIDHYINHVKDAIQQHQPQMLLLGGGVSANKYLREQVTQLQLPYLIAPLKYTSDNGAMIGFYANLLINGKNN", "aa_hexdigest": "3504ff01fa778645fb7059f8541d679e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12279.903899999987, "isoelectric_point": 9.335940742492678 }, "id": "GIHJHMOALN_175", "locus": "GCA009939745_00135" }, { "type": "cds", "sequence": "contig_1", "start": 76098, "stop": 76328, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00438.25" ], "nt": "ATGGCAAAAACAATTAAACATCCCCGATGGGGTAGATATGTAGCAGAAGCAGTTGGTCGTGGTCACCCCGATAAGATCTGTGACCAGATTGCGGACAGTATCTTAGATGAATGCATTAAGCAAAGTCCGACCAGTCACGTTGCTTGTGAGGTCTTTGCTTCCAAAAATCTGATTATGGTGGGCGGGGAAATCCTTACCACTGGTTATGTTGATGTGGTCCAAACCGGTTGA", "aa": "MAKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMVGGEILTTGYVDVVQTG", "aa_hexdigest": "5e3f238bd5d55ef4d35541e6737fd9b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00438.25", "name": "S-AdoMet_synt_N", "length": 60, "aa_cov": 0.7894736842105263, "hmm_cov": 1.0, "evalue": 8.995500879692238e-25, "score": 82.66057586669922, "start": 14, "stop": 73 } ], "seq_stats": { "molecular_weight": 8256.410599999996, "isoelectric_point": 6.243501853942871 }, "id": "GIHJHMOALN_176", "locus": "GCA009939745_00136" }, { "type": "cds", "sequence": "contig_1", "start": 76344, "stop": 76958, "strand": "+", "frame": 3, "gene": "metK", "product": "methionine adenosyltransferase", "db_xrefs": [ "COG:COG0192", "COG:H", "EC:2.5.1.6", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005737", "GO:0006556", "GO:0006730", "KEGG:K00789", "SO:0001217", "UniRef:UniRef50_P78003", "UniRef:UniRef90_P78003" ], "nt": "TTGGGTTACACCGAAAATGACTTTAGCTTTTTGAGTTGTATTAACTCCCAGTCCAGTGAAATTAACCAAGCTGTGCAAAGTAATGACGAAATAGGCGCGGGCGACCAGGGCATTACCGTTGGTTATGCTTGTAGTGAAACCGAGCAATTAATGCCCTTAGGGAGTATCGTAGCGCAAGCGCTGGTGCAACGCGCTGCTCGTATCATTGACCAATACCCCTTCATTAAACACGACATGAAGAGTCAGGTAGTGCTCAACTATACTGGTAACAAAGTTCAATGTGAATCAGTTTTAATGTCGGTACAACATACTCAGGACGTAAGTTTAGACCAACTGCGCCAAACAATTATCAACCAAGTGATCCTCCCCGTATTAACGGAGTACGGTTTAAATGATCCTAAAATCAAACACCTAGTCAATCCCGGGGGTTCGTTTGTCGTTGGGGGCCCGATGGCCGATACGGGGTTAACCGGACGCAAGATTATTGTGGACACCTATGGTCCGTACGCTAATCATGGTGGGGGGAGTTTTAGTGGTAAAGACCCTACCAAGGTCGACCGCACTGGAGCTTACTTTGCGCGCTTTATTGCTAAACACATTGTTAGTTTGGGTTGA", "aa": "MGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGITVGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHDMKSQVVLNYTGNKVQCESVLMSVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTGRKIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLG", "aa_hexdigest": "546d2582b694afbac1d70544e267a248", "start_type": "TTG", "rbs_motif": null, "genes": [ "metK" ], "truncated": "both", "pseudogene": { "start": 76098, "stop": 77249, "observations": { "mutation": [ 76326, 76956, 77094, 77193 ], "stop": [ 76326, 76956, 77094, 77193 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78003", "query_cov": 1.0, "subject_cov": 0.5326370757180157, "identity": 0.995, "score": 405.0, "evalue": 1.92e-140, "gene_start": 1, "gene_end": 204, "reference_start": 83, "reference_end": 286, "reference_sequence": "MAKTIKHPRWGRYVAEAVGRGHPDKICDQIADSILDECIKQSPTSHVACEVFASKNLIMVGGEILTTGYVDVVQTGWKVLNRLGYTENDFSFLSCINSQSSEINQAVQSNDEIGAGDQGITVGYACSETEQLMPLGSIVAQALVQRAARIIDQYPFIKHDMKSQVVLNYTGNKVQCESVLMSVQHTQDVSLDQLRQTIINQVILPVLTEYGLNDPKIKHLVNPGGSFVVGGPMADTGLTGRKIIVDTYGPYANHGGGSFSGKDPTKVDRTGAYFARFIAKHIVSLGWAEECEVSISWVFSQPLPQSIQVKCFNINKEFSEQLINQVISQYFNWSVAKIIAKLKLLDQVEYFRYAVYGHFGHQTAPWEQLSERDSLQCLIKNFQ" }, "paralog": false, "identity": 0.9895561357702349, "score": 755.0, "evalue": 8.45e-281, "description": "Internal stop codon at 76326, 76956, 77094, 77193. Nonsense mutation around 76326, 76956, 77094, 77193.", "psc": { "uniref90_id": "UniRef90_P78003", "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_P78003", "cog_id": "COG0192", "cog_category": "H", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005737", "GO:0006556", "GO:0006730" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78003", "EC:2.5.1.6", "UniRef:UniRef50_P78003", "COG:COG0192", "COG:H", "KEGG:K00789", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005737", "GO:0006556", "GO:0006730" ] }, "pscc": { "uniref50_id": "UniRef50_P78003", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78003" ], "product": "S-adenosylmethionine synthase" } }, "id": "GIHJHMOALN_177", "locus": "GCA009939745_00137" }, { "type": "cds", "sequence": "contig_1", "start": 77225, "stop": 77386, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XDC4", "UniRef:UniRef90_A0AAN4XDC4" ], "nt": "ATGTTTAATCAAAAATTTCCAATAAAAGCTCCTAAAGCACCATTTTTTAAAAAGAAAACACCACCAGTACCACCAAAGAATCCTGTGGTAACCAGCACTACCCCAGAACTCAAGAAAACCAAAATTAAAAAAGTAAAGGAAGCAGGGGATACCAATCTTTGA", "aa": "MFNQKFPIKAPKAPFFKKKTPPVPPKNPVVTSTTPELKKTKIKKVKEAGDTNL", "aa_hexdigest": "770a43b7562971f7b4b06ca4e25595b0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 77225, "stop": 77506, "observations": { "mutation": [ 77384, 77471 ], "stop": [ 77384, 77471 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN4XDC4", "query_cov": 1.0, "subject_cov": 0.5698924731182796, "identity": 1.0, "score": 102.0, "evalue": 2.44e-27, "gene_start": 1, "gene_end": 53, "reference_start": 1, "reference_end": 53, "reference_sequence": "MFNQKFPIKAPKAPFFKKKTPPVPPKNPVVTSTTPELKKTKIKKVKEAGDTNLWDKIKAKVKKHPYLFAVLVSFILLIALLGWLFAIPISQIP" }, "paralog": false, "identity": 0.9247311827956989, "score": 166.0, "evalue": 8.24e-55, "description": "Internal stop codon at 77384, 77471. Nonsense mutation around 77384, 77471.", "psc": { "uniref90_id": "UniRef90_A0AAN4XDC4", "uniref50_id": "UniRef50_A0AAN4XDC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN4XDC4", "UniRef:UniRef50_A0AAN4XDC4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAN4XDC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XDC4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5935.075700000002, "isoelectric_point": 10.352284812927245 }, "id": "GIHJHMOALN_178", "locus": "GCA009939745_00138" }, { "type": "cds", "sequence": "contig_1", "start": 77537, "stop": 77755, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02978.25" ], "nt": "ATGACGCGTGAAGAACGCCGGCACCCCAAATTAATTAATCGTGACCCATCGCGAAAACAACGCATCATTAAGGGTTCGGGACGTAAAATGGATGAACTCAATAAGCTAATGAAGGAGTGGAGTAAACTGCAAACGAAAACAGCAGAAATGGGCCGGATGCTGAAAGGTGGTAAAAATCCCTTTAGTGGCTTTGGTGGACTTGGTGGCTTAGGTTTTTAA", "aa": "MTREERRHPKLINRDPSRKQRIIKGSGRKMDELNKLMKEWSKLQTKTAEMGRMLKGGKNPFSGFGGLGGLGF", "aa_hexdigest": "f4b5c3aba86d1e43626ba913840e938b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02978.25", "name": "SRP_SPB", "length": 45, "aa_cov": 0.625, "hmm_cov": 0.9555555555555556, "evalue": 1.1478918682799941e-12, "score": 43.64171600341797, "start": 1, "stop": 45 } ], "seq_stats": { "molecular_weight": 8217.570200000002, "isoelectric_point": 11.053314018249512 }, "id": "GIHJHMOALN_179", "locus": "GCA009939745_00139" }, { "type": "cds", "sequence": "contig_1", "start": 77774, "stop": 78496, "strand": "-", "frame": 3, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "COG:COG0541", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501", "KEGG:K03106", "SO:0001217", "UniRef:UniRef50_P75054", "UniRef:UniRef90_P75054" ], "nt": "ATGTTAGTGGCACTAGACATCTACCGCCCCGCTGCGATTGACCAGTTAGCGACCCTAGCCGAACAAACCGAGTCTGTCTTCTTTGCCAAGGGTACCCAAGCCCCCGACCAAACCACCAAGGAGGCCGTTAAGACCTTTAAGGAAAGCGGTTGTCAAGCAATAATCTGTGACACGGCGGGACGGTTACAAACTAATCAGGAATTAATGGATGAGTTGGTAGCAATTAAAAACGAACTCCACCCCGATGAAATCCTAATGGTGGTCGATGGTTTAAGTGGCCAGGAAATTATTAATGTTGCCAAGGAGTTTCATAACCGTTTAAAACTCACCGGTTTTATTATTACCAAGCTTGACAGTGATGCCCGCGCTGGAGCGGCTTTGTCCTTAACGAGTTTGTTGGAAGTTCCGATTAAACTAATGGGGACATCAGAAAAACTCACAGGTCTAGAACAATTTCACCCAGAACGGATTGCCAGTCGGATCTTGGGTTTAGGGGATGTGATGACCTTAGTGGAAAAAGTGGAAGAAGTATTTGACAAACAGAGCTTAACCAAAACAGTTAGCAAGATGTTCTTGGGCAAGATGGACCTGGAAGATCTGTTGCTTTACATGGAACAGATGAACCAAATGGGTTCTGTTAGTAGTATTGTCAAGATGCTCCCGGGTAACTTAACGGTATCGGATGATCATGTAGAGAGCATTGAAGCCAAGGTTAAACTGTGA", "aa": "MLVALDIYRPAAIDQLATLAEQTESVFFAKGTQAPDQTTKEAVKTFKESGCQAIICDTAGRLQTNQELMDELVAIKNELHPDEILMVVDGLSGQEIINVAKEFHNRLKLTGFIITKLDSDARAGAALSLTSLLEVPIKLMGTSEKLTGLEQFHPERIASRILGLGDVMTLVEKVEEVFDKQSLTKTVSKMFLGKMDLEDLLLYMEQMNQMGSVSSIVKMLPGNLTVSDDHVESIEAKVKL", "aa_hexdigest": "19be4caa2a94c2bdb055ad2629b6b0c1", "start_type": "ATG", "rbs_motif": null, "genes": [ "ffh" ], "truncated": "both", "pseudogene": { "start": 77564, "stop": 78796, "observations": { "mutation": [ 77776, 78526, 78670 ], "stop": [ 77776, 78526, 78670 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75054", "query_cov": 1.0, "subject_cov": 0.5333333333333333, "identity": 0.996, "score": 453.0, "evalue": 1.64e-157, "gene_start": 1, "gene_end": 240, "reference_start": 132, "reference_end": 371, "reference_sequence": "MFKSMISSIVMRSMQKKINAQTISETDVQAVLKEIRIALLDADVNLLVVKNFIKAIREQTVGQTVEPGQDLQKWLLKVIKQELINILSQPNQEITSKRPLKVMVVGLQGSGKTTTCGKLAVWLKKQFQQKAMLVALDIYRPAAIDQLATLAEQTESVFFAKGTQAPDQTTKEAVKTFKESGCQAIICDTAGRLQTNQELMDELVAIKNELHPDEILMVVDGLSGQEIINVAKEFHNRLKLTGFIITKLDSDARAGAALSLTSLLEVPIKLMGTSEKLTGLEQFHPERIASRILGLGDVMTLVEKAEEVFDKQSLTKTVSKMFLGKMDLEDLLLYMEQMNQMGSVSSIVKMLPGNLTVSDDHVESIEAKVKLWKVLINSMTREERRHPKLINRDPSRKQRIIKGSGRKMDELNKLMKEWSKLQTKTAEMGRMLKGGKNPFSGFGGLGGLGF" }, "paralog": false, "identity": 0.9902439024390244, "score": 773.0, "evalue": 4.35e-286, "description": "Internal stop codon at 77776, 78526, 78670. Nonsense mutation around 77776, 78526, 78670.", "psc": { "uniref90_id": "UniRef90_P75054", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P75054", "cog_id": "COG0541", "cog_category": "U", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75054", "EC:3.6.5.4", "UniRef:UniRef50_P75054", "COG:COG0541", "COG:U", "KEGG:K03106", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_P75054", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75054" ], "product": "Signal recognition particle protein" } }, "id": "GIHJHMOALN_180", "locus": "GCA009939745_00140" }, { "type": "cds", "sequence": "contig_1", "start": 78668, "stop": 78889, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02881.24" ], "nt": "ATGTTTAAGTCAATGATCTCGAGCATCGTTATGCGCTCGATGCAAAAAAAGATCAACGCGCAAACGATCAGTGAAACCGATGTCCAAGCCGTTTTAAAGGAAATTCGGATTGCTCTTTTAGACGCTGACGTGAACCTGTTGGTGGTCAAAAACTTCATTAAGGCGATTCGCGAACAAACCGTTGGTCAAACCGTAGAACCCGGTCAGGACTTACAGAAGTGA", "aa": "MFKSMISSIVMRSMQKKINAQTISETDVQAVLKEIRIALLDADVNLLVVKNFIKAIREQTVGQTVEPGQDLQK", "aa_hexdigest": "bbb26c70de1b627167fec426a9fd998a", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02881.24", "name": "SRP54_N", "length": 60, "aa_cov": 0.7945205479452054, "hmm_cov": 1.0, "evalue": 1.0599991878104925e-15, "score": 53.18986892700195, "start": 16, "stop": 73 } ], "seq_stats": { "molecular_weight": 8202.591699999997, "isoelectric_point": 9.222411918640137 }, "id": "GIHJHMOALN_181", "locus": "GCA009939745_00141" }, { "type": "cds", "sequence": "contig_1", "start": 78945, "stop": 79661, "strand": "+", "frame": 3, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152", "KEGG:K03784", "RefSeq:WP_014325316.1", "SO:0001217", "UniParc:UPI0001DD6496", "UniRef:UniRef100_A0AAV5N7K0", "UniRef:UniRef50_P47295", "UniRef:UniRef90_P75053" ], "nt": "ATGACACCGCACATAAACGCAAAAAAAGATGACATTGCTAAGGTTGTTTTAATGCCCGGTGATCCCTTACGTGCTAAGTGGATTGCGGAACAATTTATGGAAAAGCCCCGGCTCGTCAACGAAGTGCGGGGCATGTTAGCTTTTACCGGTCAGTATAAGGGTAAGACCATTACCATTATGGGCCATGGCATGGGGATTCCCTCAATTGGGATTTATTCTTATGAACTGATGAAGTTCTATGAGGTTAATACCATTATCCGAATTGGTAGTTGCGGGGCGTTGCAAGATTCCTTAAACCTCCAGGACCTCATTATTGCCGCTAAGGCTTGGAGTGAGTCGATTTACGCTAACGACATGGGTGTGGAAGTGCCAGCAGACAAGATCTTAATGGCCTCCCCGCAACTAGTAGAGTTAGCTAAAAAGACCGCTAACCAACTTCAACTGGCCTTCCATGAGGGCTTGGTGTTCTGTGAGGATGCCTTCCACCAAACCAGGAAAGACGTGTTAAAGCTAGCACAAGAAAAACACGCTTTAGCAGTGGAAATGGAAGCGCATGCTCTGTACGCTAATGCCATGCTGCTCAACAAGCAAGCGTTAACGATGCTCACGGTGTCCGATTCTTTGGTAACCCATGCTGCTCTACCAGCGGAACAACGCCAAGCCACCTTTAAGAACATGGCTATCTTATCCTTGGAAATGGCTAGCCAACTCGTTTAA", "aa": "MTPHINAKKDDIAKVVLMPGDPLRAKWIAEQFMEKPRLVNEVRGMLAFTGQYKGKTITIMGHGMGIPSIGIYSYELMKFYEVNTIIRIGSCGALQDSLNLQDLIIAAKAWSESIYANDMGVEVPADKILMASPQLVELAKKTANQLQLAFHEGLVFCEDAFHQTRKDVLKLAQEKHALAVEMEAHALYANAMLLNKQALTMLTVSDSLVTHAALPAEQRQATFKNMAILSLEMASQLV", "aa_hexdigest": "8ca92d861fe0e9828a7e17179010bc50", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6496", "ncbi_nrp_id": "WP_014325316.1", "uniref100_id": "UniRef100_A0AAV5N7K0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6496", "RefSeq:WP_014325316.1", "UniRef:UniRef100_A0AAV5N7K0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N7K0", "uniref90_id": "UniRef90_P75053" }, "psc": { "uniref90_id": "UniRef90_P75053", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_P47295", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152" ] }, "pscc": { "uniref50_id": "UniRef50_P47295", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47295" ], "product": "Purine nucleoside phosphorylase DeoD-type" }, "genes": [ "deoD" ], "id": "GIHJHMOALN_182", "locus": "GCA009939745_00142" }, { "type": "cds", "sequence": "contig_1", "start": 79665, "stop": 80339, "strand": "+", "frame": 3, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "db_xrefs": [ "COG:COG0274", "COG:F", "EC:4.1.2.4", "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052", "KEGG:K01619", "RefSeq:WP_014325317.1", "SO:0001217", "UniParc:UPI0002386E36", "UniRef:UniRef100_A0AB33HM42", "UniRef:UniRef50_P09924", "UniRef:UniRef90_P09924" ], "nt": "ATGAAATTAGAATACAACCGCATTATTGACAGTACACTACTTAAAGCTGATACCTTACCCCACGAAATTGGTGCTTTATGTGCTGATGCTCATAAGTACCAGTTTTACGCAGTTTGTGTCAATCCTAGCTATGTGCGGTATGCCAAAAATATCCTTAAGGGTACGGGGGTGAAACTGTGCACTGTCGTTGGTTTTCCCTTAGGACAAACAACACAACGCCAGAAGGTGTATGAAACTAAGATTGCCATTAAGGAAGGTGCGGATGAAATTGACATGGTAATGAACATTGCCGAGTTCAAAAAGCGCTGTGCCTGTGTCATTAGTGAAATAAGGGCAGTCAAAAAGGTTTGTGGTAAGCGAACGCTCAAAGTCATTATTGAAACGGCTTTGTTAAACCAGGACGAAATTCGTGATGCCGTTAATGTCTGTATTGATGGTAACGCTGACTTTGTCAAAACTTCTACCGGGTTTTCCATGCGTGGTGCATCATTGGAAGATATCACAATTATGCGCGAAGCTAGTGGTAATCTCATTAAGATCAAAGCATCGGGTGGGGTTCAAACAGCGCAGCAATTCCTTGACTTCTTTAATGCTGGAGTTAGTCGGATTGGCACTTCCAATGCGGTCAAGATTATGGAAGAATTGCACAAACTAGAAAGCCATGAACATCGTTAA", "aa": "MKLEYNRIIDSTLLKADTLPHEIGALCADAHKYQFYAVCVNPSYVRYAKNILKGTGVKLCTVVGFPLGQTTQRQKVYETKIAIKEGADEIDMVMNIAEFKKRCACVISEIRAVKKVCGKRTLKVIIETALLNQDEIRDAVNVCIDGNADFVKTSTGFSMRGASLEDITIMREASGNLIKIKASGGVQTAQQFLDFFNAGVSRIGTSNAVKIMEELHKLESHEHR", "aa_hexdigest": "f7ea55dd7ca6bc87ac99d49cbb8e8f0d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386E36", "ncbi_nrp_id": "WP_014325317.1", "uniref100_id": "UniRef100_A0AB33HM42", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386E36", "RefSeq:WP_014325317.1", "UniRef:UniRef100_A0AB33HM42" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HM42", "uniref90_id": "UniRef90_P09924" }, "psc": { "uniref90_id": "UniRef90_P09924", "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_P09924", "cog_id": "COG0274", "cog_category": "F", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052" ] }, "pscc": { "uniref50_id": "UniRef50_P09924", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P09924" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [ "deoC" ], "id": "GIHJHMOALN_183", "locus": "GCA009939745_00143" }, { "type": "cds", "sequence": "contig_1", "start": 80326, "stop": 80463, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02885.22" ], "nt": "ATGAACATCGTTAACCTTATTAGTAAAAAGCAACGCGGCAAGGCGTTAACGGAAACAGAAATTAACTGGTTTGTCCATTCCGTTAATAACAAAAGCTTGGCCGATTATCAGGTCAGTGCCTTTTTAATGGCAGTGTGA", "aa": "MNIVNLISKKQRGKALTETEINWFVHSVNNKSLADYQVSAFLMAV", "aa_hexdigest": "2ba27ac4f98986486a333c854ad6f3ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02885.22", "name": "Glycos_trans_3N", "length": 41, "aa_cov": 0.9111111111111111, "hmm_cov": 1.0, "evalue": 3.548436955481189e-12, "score": 41.433345794677734, "start": 5, "stop": 45 } ], "seq_stats": { "molecular_weight": 5109.8771000000015, "isoelectric_point": 9.398990821838382 }, "id": "GIHJHMOALN_184", "locus": "GCA009939745_00144" }, { "type": "cds", "sequence": "contig_1", "start": 80473, "stop": 81114, "strand": "+", "frame": 1, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "nt": "ATGAATTCCAAGGAGTTGTTTTGTCTCACCAAAGCGATGGTGAAATCAGGAGAGAGCCTCCACTTTAACCACCACAGCAAGTTAAGTGTTGACAAACACTCCACTGGTGGTATTGGTGACAAGGTTTCCATTGCTTTAATTCCAATCTTAACTGCCTTAGATTACAGTGTCGCAAAACTGTCGGGTCGTGGTTTGGGCTACACTGGTGGCACAATTGACAAATTGGAAGCAGTGGGAGTAAAAACAGATTTTACACCAACAGAGGCGCAGAACTTACTAGACCAAAATGACTGCTTTATTATTGGTCAAAGTGAGGGCATTGCTCCCGTAGACAAGGTGTTGTATGCACTACGCGATACTACGGCAACTGTGGACAGTCTGCCCTTAATTGCTTCCAGTGTCATGTCGAAGAAGTTGGCAATTAACAACGACTACATCTTCATTGACCTTAAGTACGGTAAGGGCGCTTTCTGTAAAACGAAGACGATGGCTAAAGAGCTAGCGCAGTACATGTACAGTATTGCCAAGCAGTTTAAGCGCAAACTCTACATTAAACTAAGTGACATGAACCAGGTTTTGGGTAAAACGATTGGTAATGCGTTAGAGGTCTTGGAAGTAGTGCACTTCTTAAAACGGAATTGA", "aa": "MNSKELFCLTKAMVKSGESLHFNHHSKLSVDKHSTGGIGDKVSIALIPILTALDYSVAKLSGRGLGYTGGTIDKLEAVGVKTDFTPTEAQNLLDQNDCFIIGQSEGIAPVDKVLYALRDTTATVDSLPLIASSVMSKKLAINNDYIFIDLKYGKGAFCKTKTMAKELAQYMYSIAKQFKRKLYIKLSDMNQVLGKTIGNALEVLEVVHFLKRN", "aa_hexdigest": "655979a94d3c0d66a21478ef55532664", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A66C754", "query_cov": 0.9295774647887324, "subject_cov": 0.8016194331983806, "identity": 0.5, "score": 185.0, "evalue": 2.75e-55, "valid": false, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0A354QR12", "go_ids": [ "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0A354QR12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "GIHJHMOALN_185", "locus": "GCA009939745_00145" }, { "type": "cds", "sequence": "contig_1", "start": 81121, "stop": 81591, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "GTGGGTGCTGACTTTATTCAGTTGATGGAGCAAATAGTCACTGAAATCCTTATAGAAACAAAACGTGCACCAAACAAACGGGCAGCTGTAGCACTTTACCACGCTACATTAGAGGGCGAAAAACCATGGCAGCGTTTTTTAAAATTTATTGAGCTGCAAGGTAGTAGTCGGGAACGTTTTTTGGATTTAAAAGAGCTGTTTAATCCCCAGTATAAAGCGCCAGTGTTAGCATCTCAAAGTGGTACTTTGAGCTATACCAGTCCGGTGGATTTAGCCATGGTCTCGATTAGCTTAGGTGCTGGTCGAATGGTTAAAACAGATCTGATTGACCCAATGGCGGGGATTAAGTTAGTGAAACAAGCTAACGAAGTGGTAAAAGCTGGGGACACAGTCTTAGAACTTTACAGCTCCAAACCAATTACGCCTGCCCACATAGAAGCTGCGCAGCACACCATTATTATTAAGCAGTAA", "aa": "MGADFIQLMEQIVTEILIETKRAPNKRAAVALYHATLEGEKPWQRFLKFIELQGSSRERFLDLKELFNPQYKAPVLASQSGTLSYTSPVDLAMVSISLGAGRMVKTDLIDPMAGIKLVKQANEVVKAGDTVLELYSSKPITPAHIEAAQHTIIIKQ", "aa_hexdigest": "c9b5b2c9513f642a177bfe52f3babe3a", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 73, "aa_cov": 0.46153846153846156, "hmm_cov": 1.0, "evalue": 5.630747869587622e-22, "score": 72.7433090209961, "start": 83, "stop": 154 } ], "seq_stats": { "molecular_weight": 17278.940199999994, "isoelectric_point": 7.942005348205567 }, "id": "GIHJHMOALN_186", "locus": "GCA009939745_00146" }, { "type": "cds", "sequence": "contig_1", "start": 81644, "stop": 82003, "strand": "+", "frame": 2, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802", "KEGG:K01489", "SO:0001217", "UniRef:UniRef50_P75051", "UniRef:UniRef90_P75051" ], "nt": "GTGGTCAATCATGCCTACACGCCTTTTTCAAAGTTTAAGGTAGCTTGTATGTTGGTGGCCAATAACCAGGCATTTTATGGAGTCAATATTGAAAACGCTTCTTACCCAGTAACCCTGTGTGCGGAACGTAGTGCGATTGCTAACATGGTCACTAGTATCGGCAAGGCCACAATTGATTATGTCTTTGTGTACTTTGACACTAAAACTCCAACCAATTCACCTTGTGGCATGTGTCGGCAAAACATCTTTGAGTTTGCCACTGATAAGACCCAGTTATTCTGCATTGAGAAGGACAAGAGCTTTAAACAATTTACTATTCCCGAAATTTTAAAGGGAGGTTTTCGATCATATGAACAGTAA", "aa": "MVNHAYTPFSKFKVACMLVANNQAFYGVNIENASYPVTLCAERSAIANMVTSIGKATIDYVFVYFDTKTPTNSPCGMCRQNIFEFATDKTQLFCIEKDKSFKQFTIPEILKGGFRSYEQ", "aa_hexdigest": "5c45effc65b270d0ef05793c0fa32ae9", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75051", "query_cov": 1.0, "subject_cov": 0.8947368421052632, "identity": 0.992, "score": 246.0, "evalue": 1.24e-82, "valid": true, "gene": "cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_P75051", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802" ] }, "pscc": { "uniref50_id": "UniRef50_P75051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75051" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "GIHJHMOALN_187", "locus": "GCA009939745_00147" }, { "type": "cds", "sequence": "contig_1", "start": 81993, "stop": 82838, "strand": "+", "frame": 3, "gene": "manB", "product": "Phosphomannomutase", "db_xrefs": [ "COG:COG1109", "COG:G", "EC:5.4.2.8", "GO:0000287", "GO:0004615", "GO:0005975", "GO:0006166", "GO:0008973", "SO:0001217", "UniRef:UniRef50_P47299", "UniRef:UniRef90_P75050" ], "nt": "ATGAACAGTAACGCATACTTGGAAGCTCAAAGGTGGCTAAGCCATCCGCGGGTAAAACCCAACTTAAAGGAGGTCATTACGGCTATGAGTGCTGAGGAAATCGAGCACTTCTTTAGCTTAAAGAAACCGAGTTTTGGCACTGCTGGGGTACGGGGCAAAATGGCACCGGGGTACCATGGGATGAATGTGTTTTCCTATGCCTACTTAACCCAGGGTTATGTCAACTACATCCAGTCGTTAAACCCAACTAAAAAACCATTACGCTTTTTAGTAGCCAGAGACACTAGAAAACACGGGGCATTGTTTAATGGCATTGTGTGTGATGTGATTACCTCCATGGGTCATGTGGTCTATATGTTTGATAACAATGAACCAACACCAACACCATTAGTATCTTATGTGATTAAGAAGTACCACTTTGATGGTGGGGTGAATGTCACTGCTAGTCATAATCCTAAAACCGATAATGGCTTTAAGATTTACGATGGTCATGGCGCGCAGTTACTAGACTTCCAAACTGATCAATTAATCGCAATGTTACCCCCAGTGGTAACAATGTTAGATTTCGAACCACGTGGTAACAACGAGCTGTTACACTTTTTGGATAACGAAGTGGTCTACAAAAACTACTTTGATGATCTTAAGGAAAGCCTAGTAGTGGACAACGACAGCTTTAAAAACCTCCCGGTGGTCTTTACCGGGTTACATGGTACTTCGGTGAAGTTGTTACCAAGGTTTTTAACCTATTTGGGTTACAGCAACATTATTAGTGTCCAACCACAAAACGTGTTTGACGCTAACTTTGCTAATGCTGACCACCTCAACCCCGAAAGTAAGGATACCTGA", "aa": "MNSNAYLEAQRWLSHPRVKPNLKEVITAMSAEEIEHFFSLKKPSFGTAGVRGKMAPGYHGMNVFSYAYLTQGYVNYIQSLNPTKKPLRFLVARDTRKHGALFNGIVCDVITSMGHVVYMFDNNEPTPTPLVSYVIKKYHFDGGVNVTASHNPKTDNGFKIYDGHGAQLLDFQTDQLIAMLPPVVTMLDFEPRGNNELLHFLDNEVVYKNYFDDLKESLVVDNDSFKNLPVVFTGLHGTSVKLLPRFLTYLGYSNIISVQPQNVFDANFANADHLNPESKDT", "aa_hexdigest": "4dd14b0aa519f837ae19bfa0fa1c675f", "start_type": "ATG", "rbs_motif": null, "genes": [ "manB" ], "truncated": "3-prime", "pseudogene": { "start": 81993, "stop": 83141, "observations": { "mutation": [ 82836, 82926 ], "stop": [ 82836, 82926 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75050", "query_cov": 1.0, "subject_cov": 0.5072202166064982, "identity": 1.0, "score": 576.0, "evalue": 1.02e-203, "gene_start": 1, "gene_end": 281, "reference_start": 1, "reference_end": 281, "reference_sequence": "MNSNAYLEAQRWLSHPRVKPNLKEVITAMSAEEIEHFFSLKKPSFGTAGVRGKMAPGYHGMNVFSYAYLTQGYVNYIQSLNPTKKPLRFLVARDTRKHGALFNGIVCDVITSMGHVVYMFDNNEPTPTPLVSYVIKKYHFDGGVNVTASHNPKTDNGFKIYDGHGAQLLDFQTDQLIAMLPPVVTMLDFEPRGNNELLHFLDNEVVYKNYFDDLKESLVVDNDSFKNLPVVFTGLHGTSVKLLPRFLTYLGYSNIISVQPQNVFDANFANADHLNPESKDTWELARQYASNTKAKLMMAIDPDADRFAIAEWNPQTQDWHYFSGNESGVMVAYYKLKHKQFKRQPYIVTTVVSTDLVDKIAKKYGAFVKRTNVGFKFIGQAVNHFSKDNELVVAFEEAIGMMASDGLNREKDSFQAAAIMLEIARYCHNKGISLLEFYRGEIFGEFGDYYNWTVPHTIHGVNWKEKMEQVLHQLTTATIKEVVGHKITKIKNYVDINLVEYVLENGNWIKFRISGTEPKLKLYFNLSNGYLAALKHEAKKMHEFLVRLLNLDKA" }, "paralog": false, "identity": 0.9947643979057592, "score": 774.0, "evalue": 5.18e-284, "description": "Internal stop codon at 82836, 82926. Nonsense mutation around 82836, 82926.", "psc": { "uniref90_id": "UniRef90_P75050", "gene": "manB", "product": "Phosphomannomutase", "ec_ids": [ "5.4.2.8" ], "uniref50_id": "UniRef50_P47299", "cog_id": "COG1109", "cog_category": "G", "go_ids": [ "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75050", "EC:5.4.2.8", "UniRef:UniRef50_P47299", "COG:COG1109", "COG:G", "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_P47299", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47299" ], "product": "Phosphomannomutase" } }, "id": "GIHJHMOALN_188", "locus": "GCA009939745_00148" }, { "type": "cds", "sequence": "contig_1", "start": 82980, "stop": 83348, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "ATGGTGGCTTACTATAAGCTCAAACACAAGCAGTTTAAGCGTCAACCTTACATTGTAACGACGGTAGTCTCTACTGATCTAGTGGATAAGATTGCCAAGAAGTACGGGGCTTTTGTCAAGCGCACAAATGTCGGCTTTAAGTTTATTGGTCAAGCTGTTGATCACTTCTCCAAAGACAATGAACTAGTAGTGGCCTTTGAAGAAGCGATTGGTATGATGGCTAGTGATGGTTTGAACCGCGAAAAGGATTCCTTTCAAGCAGCGGCCATCATGCTTGAAATTGCGCGCTACTGTCATAATAAAGGAATTAGCTTACTAGAGTTCTACCGTGGTGAGATCTTCGGTGAATTTGGTGATTACTATAACTGA", "aa": "MVAYYKLKHKQFKRQPYIVTTVVSTDLVDKIAKKYGAFVKRTNVGFKFIGQAVDHFSKDNELVVAFEEAIGMMASDGLNREKDSFQAAAIMLEIARYCHNKGISLLEFYRGEIFGEFGDYYN", "aa_hexdigest": "6ee8b8e379ef7ade73fb9f010b5881fb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 102, "aa_cov": 0.8360655737704918, "hmm_cov": 0.9215686274509803, "evalue": 3.0111921611840937e-16, "score": 55.0925407409668, "start": 3, "stop": 104 } ], "seq_stats": { "molecular_weight": 13995.943299999992, "isoelectric_point": 8.59100856781006 }, "id": "GIHJHMOALN_189", "locus": "GCA009939745_00149" }, { "type": "cds", "sequence": "contig_1", "start": 83391, "stop": 83657, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00408.26" ], "nt": "ATGGAACAGGTATTACACCAGTTAACCACAGCTACCATTAAAGAGGTTGTGGGACACAAGATTACCAAGATTAAAAACTATGTCGACATTAACCTAGTCGAATATGTCTTGGAAAACGGTAACTGGATTAAGTTCCGCATTTCCGGTACAGAACCGAAGTTAAAGCTGTACTTTAACCTATCTAAAGGTTACTTAGCAGCTTTAAAACACGAAGCGAAAAAGATGCACGAGTTCCTAGTGCGATTGCTCAACCTCGATAAAGCTTAG", "aa": "MEQVLHQLTTATIKEVVGHKITKIKNYVDINLVEYVLENGNWIKFRISGTEPKLKLYFNLSKGYLAALKHEAKKMHEFLVRLLNLDKA", "aa_hexdigest": "bdee44ac741da2c61bdd4ad82e7d05d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00408.26", "name": "PGM_PMM_IV", "length": 34, "aa_cov": 0.38636363636363635, "hmm_cov": 0.9117647058823529, "evalue": 9.9809024087676e-07, "score": 24.284587860107422, "start": 26, "stop": 59 } ], "seq_stats": { "molecular_weight": 10238.96979999999, "isoelectric_point": 9.522512245178223 }, "id": "GIHJHMOALN_190", "locus": "GCA009939745_00150" }, { "type": "cds", "sequence": "contig_1", "start": 83625, "stop": 83879, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAATTCCTTACTTGGGTAAATACGAAATTGTCGAAGGTGACACTAAGGTCGATCAACTGCAACAATTTAGTGTTGGTAACTTAGTGGAAGTCCACTTAACTGGTGCGATTCACATTCAAGGTCAAATTAAGGCTCTATACCAAGGAACAATTAACAAAGCGGTAGTGGAAGTCGAATTAACCACCAAAACGCAGTTAATTAACTTGCCTTTAGAAAACCTAAGCTTTATCGAGGTTGAGCAATCGCACTAG", "aa": "MLIPYLGKYEIVEGDTKVDQLQQFSVGNLVEVHLTGAIHIQGQIKALYQGTINKAVVEVELTTKTQLINLPLENLSFIEVEQSH", "aa_hexdigest": "27954ce4ce3ea4788752e13f3c5fb45b", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9389.689299999996, "isoelectric_point": 4.931999015808104 }, "id": "GIHJHMOALN_191", "locus": "GCA009939745_00151" }, { "type": "cds", "sequence": "contig_1", "start": 83931, "stop": 84158, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAGGGTATTTCGGTCAATCCGAAAAAGCGGGTGTTAGTGACCAACACTGCCATTGTGGAAATGGATGCAGACAAGTTTGACGAAAAGTTTCAGTACATTCTCAAGCACAAACAAGTTAAACCCGAAGCAATTGCCCAAGTTAACGAATCTGGTGAAATCATTGATACTAACCAGTTTGCGCAAGCTCTAATGGAAGCTAATAAGGCCGAACAAGACGAATGA", "aa": "MLKGISVNPKKRVLVTNTAIVEMDADKFDEKFQYILKHKQVKPEAIAQVNESGEIIDTNQFAQALMEANKAEQDE", "aa_hexdigest": "a5de885eef23d32d9bdd17caf7f8484f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8491.595299999999, "isoelectric_point": 5.112064933776855 }, "id": "GIHJHMOALN_192", "locus": "GCA009939745_00152" }, { "type": "cds", "sequence": "contig_1", "start": 84264, "stop": 84362, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGATGAAGACCGTTATTTAAAGGTGAAGATTAGCGAGAAGAATTTACTAGGTCGTTACATCTACATTAAGATGATCTACAGTGAAGATGCATGA", "aa": "MLDEDRYLKVKISEKNLLGRYIYIKMIYSEDA", "aa_hexdigest": "b3662008d42eb2cf2cc38dec11d7d1b6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3911.5431000000017, "isoelectric_point": 6.060196113586425 }, "id": "GIHJHMOALN_193", "locus": "GCA009939745_00153" }, { "type": "cds", "sequence": "contig_1", "start": 84366, "stop": 84521, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGAAACCTCAAAGCTAAGGTAAAAGCTTTGGGATTTGACAACGAAATCTTAGATGTAAGGGTTTTGAAAGAACGCGAAGTCATCGAAGAAGTCTTTTCCTTAAAATCCGGCAAGCTGCCCCGTTCATTAAAGAACACCGCTTTTACAAAGTGA", "aa": "MRNLKAKVKALGFDNEILDVRVLKEREVIEEVFSLKSGKLPRSLKNTAFTK", "aa_hexdigest": "ee1c3213417f222481e8cba1a563eafc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5860.871099999999, "isoelectric_point": 9.991777610778808 }, "id": "GIHJHMOALN_194", "locus": "GCA009939745_00154" }, { "type": "cds", "sequence": "contig_1", "start": 84595, "stop": 84972, "strand": "-", "frame": 1, "gene": "secE", "product": "preprotein translocase subunit SecE", "db_xrefs": [ "COG:COG0690", "COG:U", "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952", "RefSeq:WP_010874425.1", "SO:0001217", "UniParc:UPI00001391AC", "UniRef:UniRef100_P75048", "UniRef:UniRef50_P47301", "UniRef:UniRef90_P75048" ], "nt": "ATGGAAAAAAAGAAACTCCCTTTTGGTTTAAAGAACAAGGAAAAACTCACCGCCTACAACGATGAGAAGATCCACGAGTTACACCGTCAACTAAAGGCCAAAATAGAAGCCAAGAAGGCCAAAGAGAAACAAGACAGTAAAACTAAAGACACAGATAAAAAAGTGGACCAAACCCCAAAGGTTAAGGTGCCTTTTACCAAGAAGTTTTCGAACCTGTGGTTTGGGATTGACAAAGAGGTTAACAAGATTGTCTGGGTAACAAGCAAGAAATTAATTACGATCTTTTTGTTAATTGTTTTAGTTAGTGCCATTATGATAGGGATCTACTTTGGCATTAACCACCTCTTTATTGCGCTTGGAGTTTTTAAAGGTAAATAA", "aa": "MEKKKLPFGLKNKEKLTAYNDEKIHELHRQLKAKIEAKKAKEKQDSKTKDTDKKVDQTPKVKVPFTKKFSNLWFGIDKEVNKIVWVTSKKLITIFLLIVLVSAIMIGIYFGINHLFIALGVFKGK", "aa_hexdigest": "98528c5cc6aa9788f61bf33ebaabfa7a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001391AC", "ncbi_nrp_id": "WP_010874425.1", "uniref100_id": "UniRef100_P75048", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391AC", "RefSeq:WP_010874425.1", "UniRef:UniRef100_P75048" ] }, "ips": { "uniref100_id": "UniRef100_P75048", "uniref90_id": "UniRef90_P75048" }, "psc": { "uniref90_id": "UniRef90_P75048", "gene": "secE", "product": "preprotein translocase subunit SecE", "uniref50_id": "UniRef50_P47301", "cog_id": "COG0690", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_P47301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47301" ], "product": "Uncharacterized protein MG055" }, "genes": [ "secE" ], "id": "GIHJHMOALN_195", "locus": "GCA009939745_00155" }, { "type": "cds", "sequence": "contig_1", "start": 85221, "stop": 85523, "strand": "-", "frame": 2, "gene": null, "product": "MPN070 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75047", "UniRef:UniRef90_P75047" ], "nt": "ATGAAAGTGATTAAGAAGCTCAATCTGCTCAATGAACTAACGATGCTGGTGTGCATCTTCTTCTTTGTGTGCACTATCTCCCTAATTGGCATTGGCATTATGTATGACCTCATTAACAGAACTTCATTGAGTGCACCACGTCGTGATCCGATTTTTCGGAACCTCAACACAGTCTTAATTGTTTTAGGGGTGTTAGAAATCCTTTTAATGGTGGCCCAATTAGTTATGTCCAATATGGCAGCCAACATTATTAACGAGGTTGCCGAAAACGTCGAGCAAAAGTTTGCTAAAGCCTTGAAATGA", "aa": "MKVIKKLNLLNELTMLVCIFFFVCTISLIGIGIMYDLINRTSLSAPRRDPIFRNLNTVLIVLGVLEILLMVAQLVMSNMAANIINEVAENVEQKFAKALK", "aa_hexdigest": "50d308f747f8078bac6dedf0b9c74f0e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 85140, "stop": 85523, "observations": { "mutation": [ 85223 ], "stop": [ 85223 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75047", "query_cov": 1.0, "subject_cov": 0.7874015748031497, "identity": 1.0, "score": 178.0, "evalue": 4.56e-56, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MKVIKKLNLLNELTMLVCIFFFVCTISLIGIGIMYDLINRTSLSAPRRDPIFRNLNTVLIVLGVLEILLMVAQLVMSNMAANIINEVAENVEQKFAKALKWSRFLPFGLLQLYCYHKIKLVTQTDNI" }, "paralog": false, "identity": 0.9921259842519685, "score": 243.0, "evalue": 5.92e-84, "description": "Internal stop codon at 85223. Nonsense mutation around 85223.", "psc": { "uniref90_id": "UniRef90_P75047", "product": "MPN070 family protein", "uniref50_id": "UniRef50_P75047", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75047", "UniRef:UniRef50_P75047", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75047", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75047" ], "product": "Uncharacterized protein MG055.2 homolog" } }, "id": "GIHJHMOALN_196", "locus": "GCA009939745_00156" }, { "type": "cds", "sequence": "contig_1", "start": 85510, "stop": 86340, "strand": "-", "frame": 1, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677", "KEGG:K07056", "RefSeq:WP_014325321.1", "SO:0001217", "UniParc:UPI0001DD649B", "UniRef:UniRef100_A0AAV5N9J1", "UniRef:UniRef50_P47302", "UniRef:UniRef90_P75046" ], "nt": "ATGCCAGCTCTTAAAGTAATTGCCACCCCCATTGGCAACATTGAGGAAGTTAGTCCGCGAGTTAAAGCGGCTTTAACCAAGTGTGAAGTGCTCTTTTGTGAGGACACCAGAGTAACCAAAAAACTCTTGGGCTTACTCGGCATTGACTTTTCAAATAAACAGTTCATTATTAACAACGAGTTTAAGGAAAAGCAAAACTTAAGTAAGGTAGCTAACTTAATCCACCAGTATCACTGTGGTTTGGTCAGTGATGCGGGTTATCCCTCAGTATCCGATCCCGGGCACATATTGGTGGAGTATGTCAGAACGCAACTACCCCAAATCGCAATAGAAGTAATTAATGGTCCTAGTGCGCTGGTTTGTGGTTTGGTAACCAGTGGCTTCCCCGAAAGTCCACTACTGTTCTTGGGCTTCTTGGACCACAAACCCACTCAAATAACCCAAACACTCAAACACTACCAAAACTTCCAAGGCACCATTGTTTTATTTGAAGCGGTACACCGTTTACAACAAACACTGGAAGTAATCCAAACGGTGTTTAGTAATACTGAAGTTTTTGTAGGGCGGGAACTGACCAAGCTCCACGAAAGCCACTACTGGTTTAATACAAGTGCACCGCTGCCAGACATTACTCTAAAGGGCGAGTTTGTGATTGTGATTAACAACCACCACACCCAACCGGTGGGGCAATATTCCGACCAACTCTTAAAACAAGAAATTACCCAATTAGTCCAAATGGGCGTTAAGGTTAAGGATGCTTGTCATTATTTAGCTAAACGATTGCAATTAAAATCAAATAAGCTCTACACCTTATTCCATGAAAGTGATTAA", "aa": "MPALKVIATPIGNIEEVSPRVKAALTKCEVLFCEDTRVTKKLLGLLGIDFSNKQFIINNEFKEKQNLSKVANLIHQYHCGLVSDAGYPSVSDPGHILVEYVRTQLPQIAIEVINGPSALVCGLVTSGFPESPLLFLGFLDHKPTQITQTLKHYQNFQGTIVLFEAVHRLQQTLEVIQTVFSNTEVFVGRELTKLHESHYWFNTSAPLPDITLKGEFVIVINNHHTQPVGQYSDQLLKQEITQLVQMGVKVKDACHYLAKRLQLKSNKLYTLFHESD", "aa_hexdigest": "e7b611fc76802afab10b3fb68d0e176f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD649B", "ncbi_nrp_id": "WP_014325321.1", "uniref100_id": "UniRef100_A0AAV5N9J1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD649B", "RefSeq:WP_014325321.1", "UniRef:UniRef100_A0AAV5N9J1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N9J1", "uniref90_id": "UniRef90_P75046" }, "psc": { "uniref90_id": "UniRef90_P75046", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_P47302", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677" ] }, "pscc": { "uniref50_id": "UniRef50_P47302", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47302" ], "product": "Ribosomal RNA small subunit methyltransferase I" }, "genes": [ "rsmI" ], "id": "GIHJHMOALN_197", "locus": "GCA009939745_00157" }, { "type": "cds", "sequence": "contig_1", "start": 86322, "stop": 86858, "strand": "-", "frame": 2, "gene": "rnmV", "product": "ribonuclease M5", "db_xrefs": [ "COG:COG1658", "COG:J", "EC:3.1.26.8", "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872", "KEGG:K05985", "RefSeq:WP_010874429.1", "SO:0001217", "UniParc:UPI00001391B7", "UniRef:UniRef100_P75045", "UniRef:UniRef50_P75045", "UniRef:UniRef90_P75045" ], "nt": "ATGGATAAAAAAACACGTCTAAAACTAGATGGGGTGATAGTGTGTGAGGGCAAAACTGACCAAGCTCGCTTACAACAGCTGTTTGATGTTAGTGTTATTACCACGAACGGTTCAGCTTTAAACCAAAGAACCATTAACCTGATTAAGGCTGTAGCTAAAAAACAACCGGTGATCCTCTTCTTGGACCCCGATGTTGCTGGTCAGAAAATTCGCCGGCAGTTAGAACAACACTTAGACAAGTATGAAAGTTGTTTTATAGCTCGGAAAGACATGAAACCCAACAGCACTAAAATTGGGGTAGCGGAAGCAACTGATGCAGCTTTAATCCAAGCGTTACAACAACGTCAGGTGTTCACTAAAACAACCCAACCCACTTTAAGCTGGGAACAATATTTGGAATTAAACCTCAACAGTAAAAGTAAACGGTTAGCGTTGTGTAACAAACTACACTTAAGCTACTTTAACCACAAGCAGCTCTTTCGCAAACTTAATTTACTCCAACTTACCTTTGACCAAGTATGCCAGCTCTTAAAGTAA", "aa": "MDKKTRLKLDGVIVCEGKTDQARLQQLFDVSVITTNGSALNQRTINLIKAVAKKQPVILFLDPDVAGQKIRRQLEQHLDKYESCFIARKDMKPNSTKIGVAEATDAALIQALQQRQVFTKTTQPTLSWEQYLELNLNSKSKRLALCNKLHLSYFNHKQLFRKLNLLQLTFDQVCQLLK", "aa_hexdigest": "44a08099613c1fb03f33930446392a15", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001391B7", "ncbi_nrp_id": "WP_010874429.1", "uniref100_id": "UniRef100_P75045", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391B7", "RefSeq:WP_010874429.1", "UniRef:UniRef100_P75045" ] }, "ips": { "uniref100_id": "UniRef100_P75045", "uniref90_id": "UniRef90_P75045" }, "psc": { "uniref90_id": "UniRef90_P75045", "gene": "rnmV", "product": "ribonuclease M5", "ec_ids": [ "3.1.26.8" ], "uniref50_id": "UniRef50_P75045", "cog_id": "COG1658", "cog_category": "J", "kegg_orthology_id": "K05985", "go_ids": [ "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75045", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75045" ], "product": "Ribonuclease M5" }, "genes": [ "rnmV" ], "id": "GIHJHMOALN_198", "locus": "GCA009939745_00158" }, { "type": "cds", "sequence": "contig_1", "start": 86837, "stop": 87823, "strand": "-", "frame": 3, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "GO:0000287", "GO:0002189", "GO:0004749", "GO:0005524", "GO:0005737", "GO:0006015", "GO:0006164", "GO:0009156", "GO:0016301", "KEGG:K00948", "RefSeq:WP_041913424.1", "SO:0001217", "UniParc:UPI000306857F", "UniRef:UniRef100_UPI000306857F", "UniRef:UniRef50_P47304", "UniRef:UniRef90_P75044" ], "nt": "ATGGATCGCCATAACCACGTTGTTTTTAGCTTATCGAAAACCCACGACTTAGTTAGTCGTATTTGTCAGAAACTCAAAATGCCCATGGGGTTAATTACCCATAATGAGTTTGCTGATGGGGAGACCTACATCCGCTTTGAGGAGTCTGTGCGCAATAAGGATGTTTTTATCTTTCAGTCTACCTGTGCGCCAGTCAACGATTCCTTAATGGAATTGTTAATAGCAATTGATGCCTTAAAACGGGGTAGTGCAAAAAGTATTACCGCCATTTTGCCGTACTATGGTTATGCACGGCAAGATCGCAAAACAATGGGACGCGAACCGATAACTAGTAAATTAGTAGCGGATTTGTTAACCACGGCTGGGGTGAGCCGCGTAGCTTTAACCGACATCCACAGCGACCAAACCCAGGGTTTCTTTAATATACCCGTGGATACATTGCAAACCTACCACGTCTTTTTAACCCGCACTGTAGAGCTGTTAGGTAAAAAAGACCTTGTGGTGGTGTCCCCCGATTATGGCGGTGTAAAGCGGGCAAGGTTAATTGCAACATCTTTGGAACTACCGCTAGCGATTATTGACAAGCGTCGCCCAGCCCACAATGTAGCGGAATCAATTAATGTCTTGGGTGAAGTAGCGAACAAAAACTGTCTAATAGTGGACGACATGATTGACACCGGTGGTACGGTAATAGCAGCAGCTAAACTACTCCGGGAACACCATGCCAAAAAGGTCTGTGTCATGGCCACCCATGGTTTGTTTAACGGTGAAGCACCGCAACGCTTCCAAAAGGCCTTTAACGAAGGCTTAGTGGACTATTTGTTTGTCTCCAACTCCATTCCTCAAACTAAGTTTGACCAGTGTCCCCAGTTTCAGGTAATTGATCTAGCACCGTTATTTGAAGAGGTGTTATTGTGTTACGCTAATAACTCTTCCATTTCTGCCATTTATACGCGCCACATTGAATGGATAAAAAAACACGTCTAA", "aa": "MDRHNHVVFSLSKTHDLVSRICQKLKMPMGLITHNEFADGETYIRFEESVRNKDVFIFQSTCAPVNDSLMELLIAIDALKRGSAKSITAILPYYGYARQDRKTMGREPITSKLVADLLTTAGVSRVALTDIHSDQTQGFFNIPVDTLQTYHVFLTRTVELLGKKDLVVVSPDYGGVKRARLIATSLELPLAIIDKRRPAHNVAESINVLGEVANKNCLIVDDMIDTGGTVIAAAKLLREHHAKKVCVMATHGLFNGEAPQRFQKAFNEGLVDYLFVSNSIPQTKFDQCPQFQVIDLAPLFEEVLLCYANNSSISAIYTRHIEWIKKHV", "aa_hexdigest": "47ccfca505d59627124ccbb8b5318610", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000306857F", "ncbi_nrp_id": "WP_041913424.1", "uniref100_id": "UniRef100_UPI000306857F", "db_xrefs": [ "SO:0001217", "UniParc:UPI000306857F", "RefSeq:WP_041913424.1", "UniRef:UniRef100_UPI000306857F" ] }, "ips": { "uniref100_id": "UniRef100_UPI000306857F", "uniref90_id": "UniRef90_P75044" }, "psc": { "uniref90_id": "UniRef90_P75044", "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_P47304", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948", "go_ids": [ "GO:0005737", "GO:0002189", "GO:0005524", "GO:0016301", "GO:0000287", "GO:0004749", "GO:0006015", "GO:0006164", "GO:0009156" ] }, "pscc": { "uniref50_id": "UniRef50_P47304", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47304" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [ "prs" ], "id": "GIHJHMOALN_199", "locus": "GCA009939745_00159" }, { "type": "cds", "sequence": "contig_1", "start": 87810, "stop": 88253, "strand": "-", "frame": 2, "gene": "smpB", "product": "SsrA-binding protein", "db_xrefs": [ "COG:COG0691", "COG:O", "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929", "KEGG:K03664", "RefSeq:WP_010874431.1", "SO:0001217", "UniParc:UPI0000136022", "UniRef:UniRef100_P75043", "UniRef:UniRef50_P75043", "UniRef:UniRef90_P75043" ], "nt": "ATGCGAGTACTTGTCAACAATCCCAGAGCGCAATATGACTATTACCTTTTAACGGGTTATTGTGCTGGCTTAGTCTTAAAAGGTAGTGAAGTCAAATCGCTAGCTTTAGGGCAAGGTAGCTTAAAGGAAGCCTATGTTTTTATTGACAAGCACGAGGTCTATATTAAAGATTTTAGCATTTCGCCCTATGCCTTTTCAGGCGAGTTCAACCACCCCTTCAAACGGGTGAAAAAGCTCCTTTTAAACCGGAATGAGATTAAACAAATTACGGCACGCCAAAAGCAAGAAGGACTTTCCATTATTCCACTTAAAGTGTTCTTTAAAAATGGCAAAATTAAAATGGAAATCTGGTTGGCCAAACCTAAGAAAAAATTTGATAAACGTGAAGCCATCAAAAGTAAAACGATCCAGCGCGAATTGCGCCAACAATATGGATCGCCATAA", "aa": "MRVLVNNPRAQYDYYLLTGYCAGLVLKGSEVKSLALGQGSLKEAYVFIDKHEVYIKDFSISPYAFSGEFNHPFKRVKKLLLNRNEIKQITARQKQEGLSIIPLKVFFKNGKIKMEIWLAKPKKKFDKREAIKSKTIQRELRQQYGSP", "aa_hexdigest": "9ab4b4e87d11e641a2c854b507837d00", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000136022", "ncbi_nrp_id": "WP_010874431.1", "uniref100_id": "UniRef100_P75043", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000136022", "RefSeq:WP_010874431.1", "UniRef:UniRef100_P75043" ] }, "ips": { "uniref100_id": "UniRef100_P75043", "uniref90_id": "UniRef90_P75043" }, "psc": { "uniref90_id": "UniRef90_P75043", "gene": "smpB", "product": "SsrA-binding protein", "uniref50_id": "UniRef50_P75043", "cog_id": "COG0691", "cog_category": "O", "kegg_orthology_id": "K03664", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929" ] }, "pscc": { "uniref50_id": "UniRef50_P75043", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75043" ], "product": "SsrA-binding protein" }, "genes": [ "smpB" ], "id": "GIHJHMOALN_200", "locus": "GCA009939745_00160" }, { "type": "cds", "sequence": "contig_1", "start": 88260, "stop": 88637, "strand": "+", "frame": 3, "gene": "wcaA", "product": "putative glycosyltransferase MG060-like protein", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.-.-", "GO:0016757", "SO:0001217", "UniRef:UniRef50_P47306", "UniRef:UniRef90_P75042" ], "nt": "ATGAAAATCTCTGTTATTATCTCCACTTACAACTGTGGTGCTTTAATTGTGAAAGCACTATGTTCATTAGTGTCAAACCAAACACCAGCATGTGAGTTAGAAGTGCTTGTGATTGATGATGGATCCATTGATAATACTAGGCAAATTATCAAAAAATTTCAAGCAAAAGTTAGCTTTACTTTAAAGTACTTTTACAAAAAAAACGGAAATTGGGGTAGTGTAATTAACTATGTCAAGGAAAACCGTTTGGCCAACGGCGATTGGATTACTGTTTTAGACAGTGATGATACCTTAAAACCTAATACACTCAACAAGTTGGCTAATTTAGTGGAAAAAGCGGATTATGACTTAGTAGTTTTTGACTACACTAAGTGCTGA", "aa": "MKISVIISTYNCGALIVKALCSLVSNQTPACELEVLVIDDGSIDNTRQIIKKFQAKVSFTLKYFYKKNGNWGSVINYVKENRLANGDWITVLDSDDTLKPNTLNKLANLVEKADYDLVVFDYTKC", "aa_hexdigest": "f298052b3cb4ab81b483fdf9067e1fd2", "start_type": "ATG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 88260, "stop": 88940, "observations": { "mutation": [ 88635, 88677, 88908 ], "stop": [ 88635, 88677, 88908 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75042", "query_cov": 1.0, "subject_cov": 0.4180602006688963, "identity": 1.0, "score": 249.0, "evalue": 2.29e-81, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MKISVIISTYNCGALIVKALCSLVSNQTPACELEVLVIDDGSIDNTRQIIKKFQAKVSFTLKYFYKKNGNWGSVINYVKENRLANGDWITVLDSDDTLKPNTLNKLANLVEKADYDLVVFDYTKCWKKIKLKIHTYPTWWKNMTRELQKQTPFCIPLGKFLKRNLFYKLPKLKEKVSFQDALYTASSLKLAKKVRHVNQSGGNYHFKRAGNSMSIPWNIKRFSAELDICKDLIRLNAQEIALVHLLRQQFRVQLKEKQIQLAVTRDFNFSGFSWYTRCFLWMVYQTMLKRYFYLQTTKQ" }, "paralog": false, "identity": 0.9867256637168141, "score": 447.0, "evalue": 1.18e-161, "description": "Internal stop codon at 88635, 88677, 88908. Nonsense mutation around 88635, 88677, 88908.", "psc": { "uniref90_id": "UniRef90_P75042", "gene": "WcaA", "product": "Uncharacterized glycosyltransferase MG060 homolog", "ec_ids": [ "2.4.-.-" ], "uniref50_id": "UniRef50_P47306", "cog_id": "COG0463", "cog_category": "M", "go_ids": [ "GO:0016757" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75042", "EC:2.4.-.-", "UniRef:UniRef50_P47306", "COG:COG0463", "COG:M", "GO:0016757" ] }, "pscc": { "uniref50_id": "UniRef50_P47306", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47306" ], "product": "Uncharacterized glycosyltransferase MG060" } }, "id": "GIHJHMOALN_201", "locus": "GCA009939745_00161" }, { "type": "cds", "sequence": "contig_1", "start": 88686, "stop": 88910, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCCGGGAACTACAAAAGCAAACACCGTTTTGTATTCCTCTAGGTAAGTTTTTGAAACGCAACCTGTTTTACAAGTTGCCCAAGCTCAAAGAAAAGGTGAGTTTTCAGGATGCCTTGTATACGGCTAGTAGTTTAAAGCTCGCTAAAAAAGTGCGTCATGTTAATCAGTCTGGTGGCAACTACCACTTTAAGCGTGCTGGTAATTCAATGAGTATTCCCTGA", "aa": "MTRELQKQTPFCIPLGKFLKRNLFYKLPKLKEKVSFQDALYTASSLKLAKKVRHVNQSGGNYHFKRAGNSMSIP", "aa_hexdigest": "d2894568273e4512d45f3cb13c0b2d79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8528.9778, "isoelectric_point": 10.389483070373537 }, "id": "GIHJHMOALN_202", "locus": "GCA009939745_00162" }, { "type": "cds", "sequence": "contig_1", "start": 88935, "stop": 89159, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACATTTGTAAAGACTTAATTCGTTTAAATGCTCAGGAAATTGCCCTAGTGCACTTATTGCGACAACAGTTTCGGGTGCAACTTAAAGAAAAGCAGATACAACTAGCGGTTACCCGTGACTTTAACTTTAGTGGGTTCTCTTGGTACACCCGCTGCTTTTTATGGATGGTATACCAAACGATGTTAAAGCGCTACTTTTACTTACAAACAACTAAACAATAA", "aa": "MDICKDLIRLNAQEIALVHLLRQQFRVQLKEKQIQLAVTRDFNFSGFSWYTRCFLWMVYQTMLKRYFYLQTTKQ", "aa_hexdigest": "43eac58589072e5fdffa7564562ad337", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9156.707199999993, "isoelectric_point": 9.755694389343262 }, "id": "GIHJHMOALN_203", "locus": "GCA009939745_00163" }, { "type": "cds", "sequence": "contig_1", "start": 89265, "stop": 89723, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTCATGAATGCCTTAATGGTGATTGTAATCGTATTGTTTGCAGCCACTCTAGGTGTGGGTACTGCTGTTGGCTTTGCCTTTGTGGCGATTGCAACCTTTATTGGTGGTTCGTTTGCTTGGAGTATGCAAAGTACCATTTTGATCCTCCCGCACGAATTCAAAGAGTACAAGCGTAGTGAAGTGTCCGTGTTGTTTGGTTACATCTGGGGCTTTGGTTATGTGATCTACACCGCCTTTGACATTACTAACTCAATGTTCTTAGAAGCACCGAAACTAGCAAACCCTGGAATGAAAGGAGTAAGCATCCTCCCAGGGGCAATTGCTGGTGTGGCGCTGTTTGCTGGTTTGTTGTTAGCAGCCATTGCGATTGTGGTAACACTGCCATCTTCGTATCTTAAGAATGGCGATGAACTGGTAAGTGAAATGACCAAAAAGTGGAAGTTAAACCAGTGA", "aa": "MFFMNALMVIVIVLFAATLGVGTAVGFAFVAIATFIGGSFAWSMQSTILILPHEFKEYKRSEVSVLFGYIWGFGYVIYTAFDITNSMFLEAPKLANPGMKGVSILPGAIAGVALFAGLLLAAIAIVVTLPSSYLKNGDELVSEMTKKWKLNQ", "aa_hexdigest": "4da9470b75af747b10a78319ffb11673", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16400.338599999985, "isoelectric_point": 7.947485160827637 }, "id": "GIHJHMOALN_204", "locus": "GCA009939745_00164" }, { "type": "cds", "sequence": "contig_1", "start": 89733, "stop": 89912, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTCCAGCGAGTTCGTTTAATGTTAAAGATGGTGTTAAAACATTAAAACCACTAGAGGGTGCTGGTGGTTACTTTGCCGGTCTCCCAACCTTAGCAATCCTCTGGGTATTAGGTTATGGTATGGGTTACATGGTGTTTTCACCATTTAACAAAACGGTTTACGATCGCAAGCGTTGA", "aa": "MSPASSFNVKDGVKTLKPLEGAGGYFAGLPTLAILWVLGYGMGYMVFSPFNKTVYDRKR", "aa_hexdigest": "309e4e3c168cbb206e83d532e66bab84", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6448.490000000002, "isoelectric_point": 9.69728603363037 }, "id": "GIHJHMOALN_205", "locus": "GCA009939745_00165" }, { "type": "cds", "sequence": "contig_1", "start": 89994, "stop": 90671, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTCCTGAAGTTTGGTTACCGTTATGCGGTCATGATTATGATGGGGCTAATGTGTCTCTGTTTCCCCTTCTTAATTATTGGTGATCCATTAGGGGGAAATAACCAGTTAACTTTATTGCGACCACTTTCAAAGGAAGTAATGGGTAAACTTTCTAGTCTATCTTCGCAATTACATGAAGGTCAATTGTTGGGGCCTGTTATGGCAGAAGGCAAGACCATGTTAGCAGATGGTCAAACTATTGATTTAGTTCAAGGTTTAGATAAAAATTTAATTGGTACCAGTTCATCAATTGCTGGTTATGCGTTGTTTATTATTTTCCGTTCCACGATTGCTATTGGTGGTACAACTTTAGTAACTTACTCCCAACCGTTAATTGCTTCACTTTCAACGCAACGTCGTAAGTCAGTGTTATCCAACGCGAACCTCTGGGGCTTTAACAGTGGGATTGTAGTTGCTTTTGTACCGTTCTTATTCCAATCTGTTCAACAAGCTGGTACTAAGTACTGGGTCTTTATCCTAACTGCTTTAATCTTGATTGGTTTTGGCATTCTCTGTGTGTTTGCTTGGTTTGAAAAGCAAATGGATCCGTTTATGCCCCAAAAGCAAACCAAGGAACAAATGCAGTTGGGTAACCAACCAAGTGCTGGTGATATCTTAAAGCGAAAAGCGACCTGA", "aa": "MVLKFGYRYAVMIMMGLMCLCFPFLIIGDPLGGNNQLTLLRPLSKEVMGKLSSLSSQLHEGQLLGPVMAEGKTMLADGQTIDLVQGLDKNLIGTSSSIAGYALFIIFRSTIAIGGTTLVTYSQPLIASLSTQRRKSVLSNANLWGFNSGIVVAFVPFLFQSVQQAGTKYWVFILTALILIGFGILCVFAWFEKQMDPFMPQKQTKEQMQLGNQPSAGDILKRKAT", "aa_hexdigest": "99fdaee3a16aeb0639d4665cbff6b736", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24641.918399999988, "isoelectric_point": 9.454304695129395 }, "id": "GIHJHMOALN_206", "locus": "GCA009939745_00166" }, { "type": "cds", "sequence": "contig_1", "start": 91495, "stop": 91929, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCCCAAAAGCAATCAAAAACCAATCAAAGCTTGAGTGTGAGACCAAGTGCGTTATCAATTTTGAAACGCAAAACAACTTGGAAGCTGTTGGCAATAGCTGGTGTAGGTACAATTCTCTTAATTAACCCGTTAACCCAAACCTGGTTTAACAGCTTGTTAGCTATTTCAGGTGCTAAAAAAGCAATTATTCCAACTGCCCGTCCAATCTTATTAATTCTCTGGGTAATGGGTTACCTCTTGGGTTACTTCTTACTGTCACCGTTTAACAAAACAATTTATGACAAGAAGCGTTGGCTCCACTTTATCTTTACGGCCAACGCCGTGTTGGTGTTGTTAATAGTAATCTTTGCTGCAACGCTTGGTTTAAATACTGTGGTTGGCTTTACGTTTGTGGGTATCTTTACCTTTATTGCTGGTGGTTTTGGTTGA", "aa": "MLPQKQSKTNQSLSVRPSALSILKRKTTWKLLAIAGVGTILLINPLTQTWFNSLLAISGAKKAIIPTARPILLILWVMGYLLGYFLLSPFNKTIYDKKRWLHFIFTANAVLVLLIVIFAATLGLNTVVGFTFVGIFTFIAGGFG", "aa_hexdigest": "c9bc3d292f9563757b86074e006a3536", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15868.987999999968, "isoelectric_point": 10.94539394378662 }, "id": "GIHJHMOALN_207", "locus": "GCA009939745_00167" }, { "type": "cds", "sequence": "contig_1", "start": 92089, "stop": 92520, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGTGAAGTTTGGTTACCGTCATGCAGTGATGATCATGATGGGACTCATGTGTGTTTGTTTTCCTTTCTTAATTATTGGTAGTCCGTTAGGTGGTCATAACGAGTTGACCTTGTTGCGACCGGCTTCAAGTGAAGTAATAAGTAAACTTACTAAAATTTCTTCCAGTTTACAACAAGGTCAACTGTTAGGTCCTGTTCAAGTGGGTAGTCAAACTATGTTGGCTGACGGCACTCCTGTTAGCTTAATTAAAGGAATTAACGGCAACGAAATTGGTACCAGTGCGTCAATGACTGGTTATGCGTTTTTCATTATTTTCCGTTCTACGATTGCTATTGGTGGTACGACCTTAATTGCTTATGCGCAACCAATTATTGCTTCCCTTTCTTCAAACCGCAAGAAATCGATTTTATCGAATGCTAACTTCTGA", "aa": "MVVKFGYRHAVMIMMGLMCVCFPFLIIGSPLGGHNELTLLRPASSEVISKLTKISSSLQQGQLLGPVQVGSQTMLADGTPVSLIKGINGNEIGTSASMTGYAFFIIFRSTIAIGGTTLIAYAQPIIASLSSNRKKSILSNANF", "aa_hexdigest": "c22c84bb559ba32bb83c1a8ea59c2d6a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15126.707199999984, "isoelectric_point": 9.757821846008298 }, "id": "GIHJHMOALN_208", "locus": "GCA009939745_00168" }, { "type": "cds", "sequence": "contig_1", "start": 92566, "stop": 92724, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGTTTGTGATTGAATGGTTTGTGATTGACTTCATTAGAGGTAGTGCGGCTTCCCTTTTAACTGAACAAACTAGTGCTGTTCCCCAGTACGGTGGTTGGTTCAGTTCTTTTTTTGTAGCGGACGCTGGTTTTATTCCAGGTCAAGCTACTAACTGA", "aa": "MLFVIEWFVIDFIRGSAASLLTEQTSAVPQYGGWFSSFFVADAGFIPGQATN", "aa_hexdigest": "f91f5412a1721c9bc8b97dfb63678239", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5691.3793, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_209", "locus": "GCA009939745_00169" }, { "type": "tRNA", "sequence": "contig_1", "start": 92964, "stop": 93052, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 76.9, "nt": "GGGAGCGTACTCAAGTGGTTAAGAGGACACCCTGCTAAGGTGTTAGATCGTTCTACGGTGCGTGGGTTCGAATCCCACCGCTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 93017, 93019 ], "id": "GIHJHMOALN_34", "locus": "GCA009939745_00170" }, { "type": "cds", "sequence": "contig_1", "start": 93283, "stop": 94350, "strand": "+", "frame": 1, "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "db_xrefs": [ "COG:COG1762", "COG:GT", "GO:0005351", "GO:0005886", "GO:0009401", "GO:0016301", "GO:0022877", "GO:0090563", "SO:0001217", "UniRef:UniRef50_P47308", "UniRef:UniRef90_P75039" ], "nt": "ATGTCAAAACTGGCATTGCCCCACATGCAAAGTGCTACCGTGTTAAAACCGTTTGTGGCGGTGTTTAAGGTCAACAATGTGGACTGGCAATCCTTAGACAACCAACCGGTTAAACTAATCTTTTTAATTGGCGTACCCAAAGATCAAGGCAACCTCCACTTGGAATTTATTTCGCAGTTTTCCAAGTTAATGTTGCAGGACGAGTTTGCCAACAAAGTGCCCAACATTCGCAGCTTTAACGGGTTGATCAATTTAATTGACAGTTTTCAACAAACTGCAGTTGCAAGTCAACCTGTTGTTAACGAAGCAGCAGCTCAGACTGAAGAGCCAAAGGACACAAACACTCAGTATGACTTTGTGGCGGTAACGGCGTGTCCCACCGGGATTGCTCACACCTTTATGGCCAAGGAAGCGCTGGAAAAGTTTGCCCGCGATCACAACCTCAAAGTTAAGGTCGAAACTCAAGGAACTGATGGGATTCAGAACCAGTTAACCGAGTCTGATTTAAACAATACCAAAGGCATTATTTTGGCCTGTGACCGCTTAATTGATTTAACGCGTTTTTACGGTCACGCAAATGTGGTGGAAGTGTCCACAACCAAAGCAATTAAAACACCACAAACAGTTTATGACCAAGTGGTAAAGAAGGAAGGCAAACTACTCGGTAATAAGTCCAGTGATAGTGCTAGTCAAACGGAACTCAAAGAAACCACTGAACAGCTCTCGTTTAAAGACTTTCACAAACGGATTTACCGCGCCATTTTAAGTGGGGTTTCTTACATGCTGCCATTTGTCGTGTTTGGTGGCATCTTAATTGCAATTGCCTTTTTAATTGACATTAATAATGCTGGCAATGCTGGTAAACAATTTGGCTCTAAAGATCCCATAGCTAACTGGTTTAAAACACTCGGTGGGTTGTCGTTTGGTTTAATTGTTCCCATTTTAAGTGCTTACATTGCCTTTGCTCTAGTCGGCCGGCAAGGGTTACTGCCCGGCTTTATTGTGGGGTTAATTTCAGCTGGTAAGTTTTTACTCAACATTGACATTGTTACTGGAAAAATTGATTGA", "aa": "MSKLALPHMQSATVLKPFVAVFKVNNVDWQSLDNQPVKLIFLIGVPKDQGNLHLEFISQFSKLMLQDEFANKVPNIRSFNGLINLIDSFQQTAVASQPVVNEAAAQTEEPKDTNTQYDFVAVTACPTGIAHTFMAKEALEKFARDHNLKVKVETQGTDGIQNQLTESDLNNTKGIILACDRLIDLTRFYGHANVVEVSTTKAIKTPQTVYDQVVKKEGKLLGNKSSDSASQTELKETTEQLSFKDFHKRIYRAILSGVSYMLPFVVFGGILIAIAFLIDINNAGNAGKQFGSKDPIANWFKTLGGLSFGLIVPILSAYIAFALVGRQGLLPGFIVGLISAGKFLLNIDIVTGKID", "aa_hexdigest": "0363d2a8b599c9665c25ea3afe23be4c", "start_type": "ATG", "rbs_motif": null, "genes": [ "fruA" ], "truncated": "both", "pseudogene": { "start": 93103, "stop": 94653, "observations": { "mutation": [ 93142, 94348, 94531 ], "stop": [ 93142, 94348, 94531 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75039", "query_cov": 1.0, "subject_cov": 0.5115273775216138, "identity": 1.0, "score": 688.0, "evalue": 1.56e-244, "gene_start": 1, "gene_end": 355, "reference_start": 61, "reference_end": 415, "reference_sequence": "MFKPLLSAELFFNWTAKDFKDKTSFLKQACRVLQDKNCIKEEQIALTALKEREAQITTGIMSKLALPHMQSATVLKPFVAVFKVNNVDWQSLDNQPVKLIFLIGVPKDQGNLHLEFISQFSKLMLQDEFANKVPNIRSFNGLINLIDSFQQTAVASQPVVNEAAAQTEEPKDTNTQYDFVAVTACPTGIAHTFMAKEALEKFARDHNLKVKVETQGTDGIQNQLTESDLNNTKGIILACDRLIDLTRFYGHANVVEVSTTKAIKTPQTVYDQVVKKEGKLLGNKSSDSASQTELKETTEQLSFKDFHKRIYRAILSGVSYMLPFVVFGGILIAIAFLIDINNAGNAGKQFGSKDPIANWFKTLGGLSFGLIVPILSAYIAFALVGRQGLLPGFIVGLISAGKFLLNIDIVTGKIDWATESKVSSGFFGAIFGGLLAAVLIIVQQRYIYRKLPQALQGIKNILFIPLLGTLVTAALFWVINIPLIYLNYGLSKFLQIMDKPYLAPLLGLVIGLMMCFDLGGPVNKAAYVFGVVSLESQNSGTVAMASAILSGMVPPLGIAIAATIRKQCFDKEELPAAYACYVMGLSFISEGAIPFVAKRPKIMLAANLIGGAVCGVLTGAFALTIRAPHGGVFVFALLKTNLEGIAGNTLQIGAGVGLALLALIVSSFISAGIIIGHNLLVVRKKTKQLVNTNA" }, "paralog": false, "identity": 0.9922480620155039, "score": 991.0, "evalue": 0.0, "description": "Internal stop codon at 93142, 94348, 94531. Nonsense mutation around 93142, 94348, 94531.", "psc": { "uniref90_id": "UniRef90_P75039", "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "uniref50_id": "UniRef50_P47308", "cog_id": "COG1762", "cog_category": "GT", "go_ids": [ "GO:0005886", "GO:0005351", "GO:0016301", "GO:0022877", "GO:0090563", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75039", "UniRef:UniRef50_P47308", "COG:COG1762", "COG:GT", "GO:0005886", "GO:0005351", "GO:0016301", "GO:0022877", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47308", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47308" ], "product": "PTS system fructose-specific EIIABC component" } }, "id": "GIHJHMOALN_210", "locus": "GCA009939745_00171" }, { "type": "cds", "sequence": "contig_1", "start": 94414, "stop": 94533, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAATTATTGTGCAGCAACGTTACATCTATAGAAAATTACCGCAAGCATTACAAGGCATTAAGAACATTCTCTTTATTCCGTTACTAGGAACGCTAGTCACAGCAGCCTTGTTCTGA", "aa": "MLIIVQQRYIYRKLPQALQGIKNILFIPLLGTLVTAALF", "aa_hexdigest": "4a6316b7bf7b970071d88b097025d84a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4471.4836000000005, "isoelectric_point": 10.446408653259276 }, "id": "GIHJHMOALN_211", "locus": "GCA009939745_00172" }, { "type": "cds", "sequence": "contig_1", "start": 94558, "stop": 95187, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACTATGGTCTGTCCAAGTTCTTGCAAATTATGGACAAACCATATTTGGCTCCCTTACTTGGTTTAGTGATTGGACTCATGATGTGCTTTGACTTGGGTGGACCGGTCAACAAAGCGGCCTATGTGTTTGGGGTAGTTTCGCTAGAGAGCCAAAACAGTGGTACGGTTGCAATGGCTAGTGCTATCTTATCGGGGATGGTGCCACCATTGGGTATTGCCATTGCTGCGACCATTCGCAAGCAGTGCTTTGACAAGGAAGAATTACCAGCAGCGTATGCTTGTTATGTGATGGGATTAAGTTTTATTAGTGAAGGGGCGATTCCTTTTGTTGCTAAACGGCCTAAGATTATGTTAGCTGCTAACCTAATTGGTGGTGCTGTGTGTGGTGTTTTAACGGGAGCATTTGCGCTAACGATTCGTGCCCCTCATGGTGGCGTGTTTGTCTTTGCCTTATTGAAAACTAACTTAGAGGGCATTGCCGGCAACACACTCCAAATCGGCGCTGGGGTTGGCTTAGCACTTCTAGCGCTAATAGTATCCAGCTTCATTAGTGCTGGCATTATTATTGGTCATAACCTGCTTGTAGTGCGTAAAAAAACAAAACAACTAGTAAATACCAATGCTTAA", "aa": "MNYGLSKFLQIMDKPYLAPLLGLVIGLMMCFDLGGPVNKAAYVFGVVSLESQNSGTVAMASAILSGMVPPLGIAIAATIRKQCFDKEELPAAYACYVMGLSFISEGAIPFVAKRPKIMLAANLIGGAVCGVLTGAFALTIRAPHGGVFVFALLKTNLEGIAGNTLQIGAGVGLALLALIVSSFISAGIIIGHNLLVVRKKTKQLVNTNA", "aa_hexdigest": "d8d4f8ca229ac002fca436f86be1a120", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21653.737600000008, "isoelectric_point": 9.38996524810791 }, "id": "GIHJHMOALN_212", "locus": "GCA009939745_00173" }, { "type": "cds", "sequence": "contig_1", "start": 95267, "stop": 96082, "strand": "+", "frame": 2, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "db_xrefs": [ "COG:COG1105", "COG:G", "EC:2.7.1.56", "GO:0005524", "GO:0005829", "GO:0008662", "SO:0001217", "UniRef:UniRef50_Q49396", "UniRef:UniRef90_P75038" ], "nt": "GTGTTAACACCCGGTGGTAAGGGAATTAACATGGCGATTGTAATGGCTTTATTTGGCATCAAACCAACCGTGTTAACCTTTTTGGGTCAACCTACTAAAGATTTATTCTTGCAGTTGTTAAAACCGTACCAACTGGACTTAGTTAGCTTTCCAGCGACTACCCAAACACGGATTAACGTGAAGCTGTTGGATGGTGCCCAAACAACTGAAATTAACGATGTCACCCCGTTAATGGAGGAACAAGCAGTCCATGAAATGATTGCTTACCTTAAGGCTAACGTTAAGCCCAATGACCTGTTGGTGTTGAACGGCCGTTTTTTACAACGCGATTTAGTTAAGTTGTTAGATGTTGCTTTTAGCTTAACCAAGTATGTTGTATTAGATGTTGATGAACCTCAACTATTACAACTGTTAAACCAGCGCCAACCCTGGTTAATGAAACCGAACCGTGATGAGTTTGTAGCAATGGTTAATGCAAATAACAGTAACGTTGACCAACAAGAGTTGGTGCAGTTAATAAAGCAGTTTCAGACTACACAGAACTTATTAATGTCAGACGGCGCGCAGGGTGCTTACTTCTTTGATCAACAGCAACTGTTATTTATGGAAGCGATTCCTCCCCAACAGTTAGTTTCCACTACGGGAGCGGGAGATACATTGCTGGGTGTTTTCCTAGCTAACTTACTGTTAGATAAGGATCCAGTGGGTAGTTTAAAGGTAGCGGTTAACTATGCTAGTGCTACGATTAGCAAGCTCGGTGTGGTCAACAGTAATGACCAAATAGTGCTAAAAGCTACTAATTATTACTATTTATAA", "aa": "MLTPGGKGINMAIVMALFGIKPTVLTFLGQPTKDLFLQLLKPYQLDLVSFPATTQTRINVKLLDGAQTTEINDVTPLMEEQAVHEMIAYLKANVKPNDLLVLNGRFLQRDLVKLLDVAFSLTKYVVLDVDEPQLLQLLNQRQPWLMKPNRDEFVAMVNANNSNVDQQELVQLIKQFQTTQNLLMSDGAQGAYFFDQQQLLFMEAIPPQQLVSTTGAGDTLLGVFLANLLLDKDPVGSLKVAVNYASATISKLGVVNSNDQIVLKATNYYYL", "aa_hexdigest": "a6d4e6e3c937ecfd1a7eed9676f574e1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75038", "query_cov": 1.0, "subject_cov": 0.9033333333333333, "identity": 0.9890000000000001, "score": 516.0, "evalue": 4.38e-184, "valid": true, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "ec_ids": [ "2.7.1.56" ], "uniref50_id": "UniRef50_Q49396", "cog_id": "COG1105", "cog_category": "G", "go_ids": [ "GO:0005829", "GO:0008662", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_Q49396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49396" ], "product": "Putative 1-phosphofructokinase" }, "genes": [ "fruK" ], "id": "GIHJHMOALN_213", "locus": "GCA009939745_00174" }, { "type": "cds", "sequence": "contig_1", "start": 96090, "stop": 96251, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574865.1", "SO:0001217", "UniParc:UPI0001DD64A0", "UniRef:UniRef100_A0AB38W8P1", "UniRef:UniRef50_A0AB38W8P1", "UniRef:UniRef90_A0AB38W8P1" ], "nt": "ATGTCAACTAAAGAACTAGCCAAACAGCCAAAGGTCAATCAAGAGAGGGAATCGATTTTGTTACCCGTTTTGCTAGCTGTTTTCAGTATGCTCTTCTTTATGCTACTGTTAGCTTTAGTGTTGTTGTACGCTATTCCAGGTAAAGTTAATAACTTGAACTAG", "aa": "MSTKELAKQPKVNQERESILLPVLLAVFSMLFFMLLLALVLLYAIPGKVNNLN", "aa_hexdigest": "d439b785fba30d91bfa41ab139de3492", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64A0", "ncbi_nrp_id": "WP_014574865.1", "uniref100_id": "UniRef100_A0AB38W8P1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64A0", "RefSeq:WP_014574865.1", "UniRef:UniRef100_A0AB38W8P1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8P1", "uniref90_id": "UniRef90_A0AB38W8P1" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8P1", "uniref50_id": "UniRef50_A0AB38W8P1" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8P1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5973.246899999998, "isoelectric_point": 9.398732948303223 }, "id": "GIHJHMOALN_214", "locus": "GCA009939745_00175" }, { "type": "cds", "sequence": "contig_1", "start": 96319, "stop": 97425, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGCAACTTAATTAAAAACGTTTTTCGCTCTTTTCGCACCGCTAAGATTGCCTTAATTGCGCTTACCTTTTTAATCTTTGTCGCGGTTGGTAGTTTCGTGTTATTAAACAATACCGTTAATAACTTTAACGCTTCCTTTCAACACGTTACCGAACAAGGACAATTGTCTAACGCCATTATTAACGAGCGCTATGACTTTGGTAAACTCGAGTTCCAAAAAACGAGTAATGATGCTACTAGTTCTGCCCAACCTAGTGTTAGTACTCGAAGTACTTTAACTGCCCAAAAAGCAAATGGCGCTAATTCCAGTTTTACTCTGACCTTAACACCTGACTCACGGACTTCATTTATTAACAACGCACTGCAACTAAAACCGGATGTTTATAACAGTTTAGTTACCGTTACTTTTAGTTATGGCAGTGAATCTGATCAAACCAAGAAAACCCAAATAGAAGAACAGTCCAAGTTAATTGCCGCAAACAATTTAAGCCGGGCATTACAAACCGATAAGGAGTTATTAGTTACGGGACAGTTAGAACAGTTAAAGGCTACCTTTCGCACTTATAAGGCAATTAACATTACTGATCAAGGAGTGTTCAAGAAGCTCATAGCCTCTGAACCGACCGATCAAGTTAACCGTCTCGTTCTGTTTGACGGACATCAACTAGCACGTTCCCGCCAAGTCGAATTTAACGAGCTGGTTAGTCAGTTTACCCAGGTGCAAGCTAAGGGTAAAGATCAGTTATCCACTACTCTAAAGAATGGTAATTACGAACAACTTTTGCAAGCGATCGCTAACAACTTTGGTAGTAGTGAAACTGAGTTACTTAAAGAAATTCAAGATGCTTTAAAGCAAACGAATAGTTCCACCACCCTTGCCCAAACCCAAAGTAGTCAACAATTTAGTAAGTTAAAAAACCTGTTTGATTCTGGTAGTACCTTTACTAACATTGCCGGCAAACTCACGTTGCAGTGGATGGAAGGTACAAACAAAAAGCAACTGGTAATCTTTGACCCGAGTAGCTATGAAACGGTAGTAGCACCGGGTAACTGGAGTTACGAACAGCAACAGGGCAAAGCGGTGTATCAGGACATTAACCACTGA", "aa": "MRNLIKNVFRSFRTAKIALIALTFLIFVAVGSFVLLNNTVNNFNASFQHVTEQGQLSNAIINERYDFGKLEFQKTSNDATSSAQPSVSTRSTLTAQKANGANSSFTLTLTPDSRTSFINNALQLKPDVYNSLVTVTFSYGSESDQTKKTQIEEQSKLIAANNLSRALQTDKELLVTGQLEQLKATFRTYKAINITDQGVFKKLIASEPTDQVNRLVLFDGHQLARSRQVEFNELVSQFTQVQAKGKDQLSTTLKNGNYEQLLQAIANNFGSSETELLKEIQDALKQTNSSTTLAQTQSSQQFSKLKNLFDSGSTFTNIAGKLTLQWMEGTNKKQLVIFDPSSYETVVAPGNWSYEQQQGKAVYQDINH", "aa_hexdigest": "c29dac8e35c6acd2a0f398e27f2abc18", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 40980.39510000004, "isoelectric_point": 9.000382804870608 }, "id": "GIHJHMOALN_215", "locus": "GCA009939745_00176" }, { "type": "cds", "sequence": "contig_1", "start": 97495, "stop": 97752, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGTCGACAAAATTGAGTACCTCATCTTGGGAGTGGGAATTACACCAGACTTTGTCTACCCCGTCTTTAGTGAATCACTGTTAATTCCCAATACGGCTAACGAGCAGTTGTATTATGTCAACCAAACTGGTTATGAACGTACCGCTAGCTCCTTTTTAACTAATCCCACCGAAACTGGCATAGTAGCACGGTTACCTAACCTCAACCATGACCTTGATGTAATTAACCAGTGGGCTGTTAAAAACATGTCCTGA", "aa": "MLVDKIEYLILGVGITPDFVYPVFSESLLIPNTANEQLYYVNQTGYERTASSFLTNPTETGIVARLPNLNHDLDVINQWAVKNMS", "aa_hexdigest": "2f3edb560e1258c6123ebab8a737b2cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9577.764099999995, "isoelectric_point": 4.395041084289551 }, "id": "GIHJHMOALN_216", "locus": "GCA009939745_00177" }, { "type": "cds", "sequence": "contig_1", "start": 97894, "stop": 98211, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "GTGGCCATCTTTGTCACCATTTTAATCTTGGTTAGTTACCTCAAAAAGAACACGGAACAAATCGGCATCTTAAAGGCTAATGGGGTGAGTGGCCGGAAGATTAACTTAAGCCTGTTAATCTTTAGTTTAATCCCGGGGATAGTGGGGTCCTTTATTGGTTATACGTTAGGTATTAGTATGCAAAACGTGGCCATTAACCTCTTTAGTAACTACTGGTTTATCCCCACGCAAACTTCGAGTTTCTCGGTGTTAGGGTTACTCTTCTTTGCCTTATTAGTGGTTCTTATGATGAGTGGGATTAGTCTGTTTGTGGGGTGA", "aa": "MAIFVTILILVSYLKKNTEQIGILKANGVSGRKINLSLLIFSLIPGIVGSFIGYTLGISMQNVAINLFSNYWFIPTQTSSFSVLGLLFFALLVVLMMSGISLFVG", "aa_hexdigest": "5f22642b17d0925359a7c7a29844e4e6", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 102, "aa_cov": 0.9619047619047619, "hmm_cov": 1.0, "evalue": 6.298402079896276e-16, "score": 54.036476135253906, "start": 2, "stop": 102 } ], "seq_stats": { "molecular_weight": 11409.65179999999, "isoelectric_point": 9.873542594909669 }, "id": "GIHJHMOALN_217", "locus": "GCA009939745_00178" }, { "type": "cds", "sequence": "contig_1", "start": 98317, "stop": 98532, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCAGATTTCGTGTCGCTTTAGCCTTTAATGCGCCGTGGAAACTGGTCTTTTTAACCTTAATGAGTGCACTAACGATGATGATCCTAAACCTTAGTTTTGCCACTAAGGATGCCTTTCAGGTAGCACAAACAAAGACTACTTTAACCCAATCGAACCACCAGTACGAGTTTGAACTAGCATCGCCAACGGAGCAGAGTGGCTTGCTCAAGTGA", "aa": "MTRFRVALAFNAPWKLVFLTLMSALTMMILNLSFATKDAFQVAQTKTTLTQSNHQYEFELASPTEQSGLLK", "aa_hexdigest": "721e075f6949e99f2db9ca17c102f9d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8043.3189999999995, "isoelectric_point": 9.397636985778806 }, "id": "GIHJHMOALN_218", "locus": "GCA009939745_00179" }, { "type": "cds", "sequence": "contig_1", "start": 98731, "stop": 99333, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACTATTCACTGGGTTTTGGTAACGTTTCTTCCAATCCGTGGAAGCTGAGTGAAACATTAATGCCCGCCAATCAAGCATCCGCTTCCAACACGGCTTTCCAAAACTTTTTAAAGGCGATTATTGTAATTGACCCTAACGAGGGCGGTAAGTACATTAAACAAACTAAGGATGAAGTTACTGTGCGTTTTTTATACGAAATAGACAGTAGTAAAGCTTTAAAAAACGGCAACACCAAATCAGTTAATAAAATACAAAAAAGTACCGCAGCACCCCAAGCCCAACAAGGACAAAGTAACCAGTTAATGCTTGACAATGATTTTGCTAAATTCTTGTACAAGCAGTTTAACCTAATCAAGGAAGGGGGCAAAGCAGATCCGGTTGATCTCAATAACTTTGACTTTGCTAATCAGAATCAGATTAAGCGTTTTTACAGTAAATACAACGCCTTACCACCGGTAGATTACAAGCTGGCCTTTAACGTGATCGGTCTCCCTAAAGAAACAATTGCCAACCAAAAGGATGCGCCCCTGTTTGGCTTTTTAACCTTGCAAGCGCAACTGGGCAATGACGCGATTAAGATTAAGGGGATTAAAGACTGA", "aa": "MDYSLGFGNVSSNPWKLSETLMPANQASASNTAFQNFLKAIIVIDPNEGGKYIKQTKDEVTVRFLYEIDSSKALKNGNTKSVNKIQKSTAAPQAQQGQSNQLMLDNDFAKFLYKQFNLIKEGGKADPVDLNNFDFANQNQIKRFYSKYNALPPVDYKLAFNVIGLPKETIANQKDAPLFGFLTLQAQLGNDAIKIKGIKD", "aa_hexdigest": "2813de51a8f8e0d84be81da9514a6e04", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22253.004199999996, "isoelectric_point": 9.204554176330568 }, "id": "GIHJHMOALN_219", "locus": "GCA009939745_00180" }, { "type": "cds", "sequence": "contig_1", "start": 99361, "stop": 100479, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F", "UniRef:UniRef90_UPI0011815B2F" ], "nt": "GTGTTGCGTACCAATGAGGACGAGATTATTAACCAGCGCTTGTTTAGTAAACCCAACGGTATTATTATCAACAGTTCAGCTGCTAAAAAGTACCGCTTAAAGGAAGGTAGTCAGCTCCATGTCAAAATTCACAACGGTTACAAACGAGTGAATGCTAAGTTAGTTAACCAAGATCCCACTTTAACTGCCACCTTTAACATTGTTGGCATTAACAATTCGGTGCACGAACCGGAGTTTTACACGAGTTACAAAACGGCAGCAACACTATTGCAATACCCCAATGAATGGATCGAAAAGCAGTTACCCTTTAACAGCTTTTATGCCAATTCGATCTTGCCCTTTACCCAGTCTACTTCCCTCTTTTCCGAATCTGGCTTATTCCCTGGTACCAGTAGCTTTGCCGCTAACAACACGGTGCTTACTGAAGTAATTAAGAAAACGATTGAAGGTGGTAAAACTAACAGTAAAACTGATGTGCAAAAGCGCGCTGCTCAAGCTACTGCTAACGGTAAGGATAACAGTAGTCAAAACAACAAGATGAATTATGAGCGCTTACAAAAGGCACTGGGCATTACCAGTGATTTAGATAGCTCTAAAGCCGGTGAATACGCCACCATCTTAGAGCGGGTGTATGATGGTTTGCCATACAACTCCACTATTAGCTTTATTAGTAATGTGCAGGCCAACGATGCCTTGTTTGCTAACATTGCTAACACCACCCAGCAAATCCAAACAGCGGCCATTGGCATTATTATTCCGATTATTATGCTAATTGTGTTGTTAGTATCAACTACCCTAATTCAGGAATTGAAGAAGATTGCGATTCGTTTAAAAGCATTGGGCTATTCCAACCCGAAGATCTTGCTGTCCTTCTTGTCGATTTACCTCCCGTTATTTATCTTTGGTTTGTTAATTTCGACCCCAATTTCGATTTATCTAATTGCACTCCACAATCAAGTTATTTTCTCCAGTGCTGCCATCTTACTGGAAGCCACATTAAACTTCCCCACGGTCATTCTGTCGATGTTAGTGTTAAGTGGGGTGTTATCAATTACCTTTGTGTTGAATTGGTTGGAGTTGAACAAAATCAAAATTGCCAAGGAGATTAAGAATAGCTAA", "aa": "MLRTNEDEIINQRLFSKPNGIIINSSAAKKYRLKEGSQLHVKIHNGYKRVNAKLVNQDPTLTATFNIVGINNSVHEPEFYTSYKTAATLLQYPNEWIEKQLPFNSFYANSILPFTQSTSLFSESGLFPGTSSFAANNTVLTEVIKKTIEGGKTNSKTDVQKRAAQATANGKDNSSQNNKMNYERLQKALGITSDLDSSKAGEYATILERVYDGLPYNSTISFISNVQANDALFANIANTTQQIQTAAIGIIIPIIMLIVLLVSTTLIQELKKIAIRLKALGYSNPKILLSFLSIYLPLFIFGLLISTPISIYLIALHNQVIFSSAAILLEATLNFPTVILSMLVLSGVLSITFVLNWLELNKIKIAKEIKNS", "aa_hexdigest": "e2a1e0215a876d97c17815cb3d841804", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0011815B2F", "query_cov": 1.0, "subject_cov": 0.8551724137931035, "identity": 0.997, "score": 679.0, "evalue": 6.56e-245, "valid": true, "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI0011815B2F" }, "pscc": { "uniref50_id": "UniRef50_UPI0011815B2F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F" ], "product": "ABC transporter permease" }, "genes": [], "id": "GIHJHMOALN_220", "locus": "GCA009939745_00181" }, { "type": "cds", "sequence": "contig_1", "start": 100479, "stop": 101870, "strand": "+", "frame": 3, "gene": "lolD", "product": "Putative ABC transporter ATP-binding MG065-like protein", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75612", "UniRef:UniRef90_P75612" ], "nt": "ATGCGATACTTTGCCCTCACTGATTTAACCAAGCCCACTGCAAAATTTACCACTGATGCCATGGAACTGCGTCAGATATCCAATGCTTACCTCAACCAAGCCCAAGCATATTTGCAAAAGGGTTTAAAGCAACTCAAAAAGGATTACAAGAATGCCATTTTGTATACCCCCAAAACGGAGTACAAGCGCTTTTTGAAATGGAAGCAAACTTTTCTTCAAGATTTAAACCAAACCCAAAAGCGCTTCTTTATTGTTCGTGCCCAACATTTTTCTTACGTGTTGTTGTTTAACTTATTGGATGAACAGGTGGAAGCGGTGATTGCGACCTTTAACAACTTCTTGGATGAACACCGCTTGGAGGCACAATCCAAGCAGTTTGATTTAGACACAGCTGTCAATGAACTGCATGATTACTTTGACCAACTGCAAAAGAACACCGCTTACAGCGAGGATTTACCAACTCACCTCACGCAAAAAACCGAGCAATTAATTAACGCTCGTAATACCCAGTTAACGAATTTGTTAAACAAGATTGCTACTACCAAACCACCCCTAAACAAACAGCAACGTTTGTTAGCCAGTTTTCGCAATTACCACGAACACCTCTTTTTAAAGAACGAGGTCAAAAAGGTGACTTGGTTAAACGAACCACGCGCCAAAAAAGAGTCTGTTACCCCTGATGAAGAACACATTATTGAACTTAAAAACGTTTACAAGTACATTACTAATGGGGTGACTACCAATGCCGTTTTAAAGGGCATAGATCTCAAACTCAAAGCCCATGATTTCATTGTGATTTTAGGTCCCTCTGGCTCGGGGAAAACGACCCTGCTCAACATTATTTCCGGGATGGACCGGCCATCAAGCGGATCTGTTGTCGTTAATGGTCAGGAAATGATTTGCATGAACGACCGTCAGTTAACTAACTTTCGCCGCAACTATGTCGGTTACATCTTCCAGCAGTACGGGCTTTTACCCAACCTAACTGTAAGGGAAAACGTTGAAGTGGGTGCTAACTTGCAACGTAACCCTGATAAGCGGATTAACATTGATGAACTGTTAGAAGCAGTAGGGATGAAACACCTCCAAAAGAAACTCCCCAACGAGTTGAGTGGTGGACAACAACAACGGGTCTCGATTGCACGGGCGTTTGCTAAAAATCCCTTACTCATTTTTGGTGATGAACCAACTGGGGCTTTGGATTTGGAAATGACCCAAATTGTGCTCAAACAGTTTTTAGCCATTAAACAACGCTATAAAACCACGATGGTCATAGTGACGCACAATAACCTAATTGCCCAACTAGCCGATTTAGTCATTTATGTGGCAGATGGCAAAATTCAAGCACTGCAAGCAAACCCTAATCCCAAACAGGTTGAGGACATTAACTGA", "aa": "MRYFALTDLTKPTAKFTTDAMELRQISNAYLNQAQAYLQKGLKQLKKDYKNAILYTPKTEYKRFLKWKQTFLQDLNQTQKRFFIVRAQHFSYVLLFNLLDEQVEAVIATFNNFLDEHRLEAQSKQFDLDTAVNELHDYFDQLQKNTAYSEDLPTHLTQKTEQLINARNTQLTNLLNKIATTKPPLNKQQRLLASFRNYHEHLFLKNEVKKVTWLNEPRAKKESVTPDEEHIIELKNVYKYITNGVTTNAVLKGIDLKLKAHDFIVILGPSGSGKTTLLNIISGMDRPSSGSVVVNGQEMICMNDRQLTNFRRNYVGYIFQQYGLLPNLTVRENVEVGANLQRNPDKRINIDELLEAVGMKHLQKKLPNELSGGQQQRVSIARAFAKNPLLIFGDEPTGALDLEMTQIVLKQFLAIKQRYKTTMVIVTHNNLIAQLADLVIYVADGKIQALQANPNPKQVEDIN", "aa_hexdigest": "08a651a5d28d33956c8c41142d0fd463", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75612", "query_cov": 1.0, "subject_cov": 0.9956989247311828, "identity": 1.0, "score": 894.0, "evalue": 0.0, "valid": true, "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG065 homolog", "uniref50_id": "UniRef50_P75612", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75612", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75612" ], "product": "Putative ABC transporter ATP-binding protein MG065 homolog" }, "genes": [ "lolD" ], "id": "GIHJHMOALN_221", "locus": "GCA009939745_00182" }, { "type": "cds", "sequence": "contig_1", "start": 101897, "stop": 102511, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00456.26" ], "nt": "ATGAAAAACCTGTTTGCTTGCCAACACTTAGCGTTAAGTGCTATTCAACACGCTAAGGGTGGTCATGTGGGAATGGCCTTAGGTGCTAGTCCTATTTTGTACACCTTATGGACTAAACACATCCAGTTTAACCCTAACTGTCCCAAATGGATTAACCGGGACCGTTTAGTGATGAGTGCAGGTCATGGTAGTATGGCCTTATACCCGATCTTACACTTTGCGGGGTTAATTACGAAGCAGGAAATGCTCCATCACAAATACGGGCAAGTCAACACCTCTTCCCATCCCGAGTATGCCCCTAATAACTTTATTGATGCGTCCACTGGTCCCTTAGGACAAGGCCTAGGAATGGCTGTCGGGATGGCCTTGACACAACGGGTACTAGCAGCTGAATTTAAAGCGTTAAGTCCCAAACTGTTTGACCACTTTACCTATGTGGTTGTGGGTGATGGTGATCTCCAGGAAGGGGTGAGTTATGAGGTAGCTCACTTAGCAGGGGTCTACCAGTTAAATAAGCTAATAGTGCTCCATGATTCCAACCGGGTGCAGATGGACAGTGTCGTGCGTGATGTGTCCTTGGAAAACCTGCAAACCCGGTTTACCAACATGGGGTGA", "aa": "MKNLFACQHLALSAIQHAKGGHVGMALGASPILYTLWTKHIQFNPNCPKWINRDRLVMSAGHGSMALYPILHFAGLITKQEMLHHKYGQVNTSSHPEYAPNNFIDASTGPLGQGLGMAVGMALTQRVLAAEFKALSPKLFDHFTYVVVGDGDLQEGVSYEVAHLAGVYQLNKLIVLHDSNRVQMDSVVRDVSLENLQTRFTNMG", "aa_hexdigest": "2e6a12f53cfc188c3415e6b1af99b6bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00456.26", "name": "Transketolase_N", "length": 198, "aa_cov": 0.9656862745098039, "hmm_cov": 1.0, "evalue": 1.8079122876555216e-62, "score": 206.6949920654297, "start": 7, "stop": 203 } ], "seq_stats": { "molecular_weight": 22438.606300000003, "isoelectric_point": 7.886175727844237 }, "id": "GIHJHMOALN_222", "locus": "GCA009939745_00183" }, { "type": "cds", "sequence": "contig_1", "start": 102599, "stop": 102853, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCACACGACAATTGCTAAAAACACCACCTTGGAAGACCAACCCGCTGGTCACTGGTTTATTCCTACCGACAAGGACTTTGCGCGCTTTAACAGCAACACCAAGACTAACTTTACCCCGTTTGAATATCCCCAAACGGTGTATGACTTCTTTCACAAGCAGGTCATAGCACGCCAAGCTAAACCAGTGCAAGCTTACAAGGAATTACTGGAAAAACTAAAGGACAAACCGCTGTATACTAAGTTTATTAATTGA", "aa": "MHTTIAKNTTLEDQPAGHWFIPTDKDFARFNSNTKTNFTPFEYPQTVYDFFHKQVIARQAKPVQAYKELLEKLKDKPLYTKFIN", "aa_hexdigest": "8e35bb182d4d48773d49830a5ca94b70", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9954.206299999994, "isoelectric_point": 9.222992134094238 }, "id": "GIHJHMOALN_223", "locus": "GCA009939745_00184" }, { "type": "cds", "sequence": "contig_1", "start": 102875, "stop": 103327, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02779.29" ], "nt": "TTGTACCTCAACCAGTTAGATGAACGCAAGGTAGCACAAACCAATGCGGCAACCCGTAACTACCTCAAGGACTTTTTAGGCCAAATTAACAACAGTAATTCCAACTTGTACTGTCTCAATGCTGATGTAGCACGTTCGTGTAACATTAAGTTAGGTGATGATAATTTACATACCAATCCGCATTCGCGCAACATTCAGGTGGGTATTCGGGAATTTGGTATGTCCACCATTATGAACGGGATGGCACTCCATGGTGGGGTCAAAGTGATGGGTGGTACCTTCCTAGCGTTTGCTGATTATTCCAAACCCGCAATTCGCTTAGGGGCGTTAATGAACCTCCCCACCTTTTATGTGTACACCCATGACTCTTACCAAGTCGGTGGTGATGGACCAACCCACCAACCGTACGATCAACTCCCGATGTTAAGGGCCATTGAAAACGTTCAGGTTTGA", "aa": "MYLNQLDERKVAQTNAATRNYLKDFLGQINNSNSNLYCLNADVARSCNIKLGDDNLHTNPHSRNIQVGIREFGMSTIMNGMALHGGVKVMGGTFLAFADYSKPAIRLGALMNLPTFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVQV", "aa_hexdigest": "41ea7780e1f6ce05a0b2adb71f09525b", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02779.29", "name": "Transket_pyr", "length": 137, "aa_cov": 0.9, "hmm_cov": 0.9927007299270073, "evalue": 6.097285001954609e-36, "score": 119.15585327148438, "start": 16, "stop": 150 } ], "seq_stats": { "molecular_weight": 16669.72029999999, "isoelectric_point": 6.952681922912597 }, "id": "GIHJHMOALN_224", "locus": "GCA009939745_00185" }, { "type": "cds", "sequence": "contig_1", "start": 103373, "stop": 103843, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22613.1" ], "nt": "TTGTTAAGCCAAGACCAAACTAACGTATTAATTTTGACCCGTCAAGCTTTGCCTTCCCTAGAACAATCTGATAGTGTACAGACCTTGAAAGGGGGCTATATTATTAGTAATAGAAAACAACCCGATGTCATTGTGGCCGCTAGTGGCAGTGAAGTTCAGCTGGCATTGCAACTAGAACAAGCTCTAAACGAACAACAATTAAAGACGCGGGTGGTGTCAGTGCCCAACATTAACATGTTGTTAAGTCAACCCCAAAGCTACTTGCAACAACTGTTTGATCCTAACAGTGTTTTACTCACCTTAGAAGCGAGTGCTAGCATGGAGTGGTATGCACTAGCAAAATACGTCAAAAAGCACACCCACTTAGGTGCTTTTAGCTTTGGTGAGTCCAATGACGGGCAAGTCGTGTATGAACACAAGGGCTTTAATGTGACTAACTTACTCAAGTTGATTAAAACTCTAAAGAGTTAA", "aa": "MLSQDQTNVLILTRQALPSLEQSDSVQTLKGGYIISNRKQPDVIVAASGSEVQLALQLEQALNEQQLKTRVVSVPNINMLLSQPQSYLQQLFDPNSVLLTLEASASMEWYALAKYVKKHTHLGAFSFGESNDGQVVYEHKGFNVTNLLKLIKTLKS", "aa_hexdigest": "5a3123250bc381e5427d9d4d9ec847a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22613.1", "name": "Transketolase_C_1", "length": 115, "aa_cov": 0.7243589743589743, "hmm_cov": 0.9826086956521739, "evalue": 4.161304380936107e-28, "score": 93.36075592041016, "start": 31, "stop": 143 } ], "seq_stats": { "molecular_weight": 17330.6251, "isoelectric_point": 6.829852867126465 }, "id": "GIHJHMOALN_225", "locus": "GCA009939745_00186" }, { "type": "cds", "sequence": "contig_1", "start": 104399, "stop": 104527, "strand": "+", "frame": 2, "gene": null, "product": "DUF31 family putative serine protease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F4E8A2E", "UniRef:UniRef90_UPI001F4E8A2E" ], "nt": "GTGATTGAGCAGAAGCTCAAGGAAGATTCGCTGTTGTTTATCCAAACGAGTACGTTACCTAAAACAGCATACGCAGCAATTGATCCGGTGAACTTTAGCTATAACCCAACCCGTACCAACGGTTTTTGA", "aa": "MIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGF", "aa_hexdigest": "2f1356b19045b9a466b124ba6dfd607d", "start_type": "GTG", "rbs_motif": "AAAA", "genes": [], "truncated": "both", "pseudogene": { "start": 104283, "stop": 104538, "observations": { "deletion": [ 104316, 104523 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F4E8A2E", "query_cov": 1.0, "subject_cov": 0.49411764705882355, "identity": 0.976, "score": 85.5, "evalue": 6.18e-21, "gene_start": 1, "gene_end": 42, "reference_start": 40, "reference_end": 81, "reference_sequence": "MVGVNHILTSKKTQSFLLGKYTKPNVQLVKTNYEKPEDAVIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGFLNSR" }, "paralog": false, "identity": 1.0, "score": 160.0, "evalue": 8.43e-53, "description": "Frameshift due to deletion around 104316, 104523.", "psc": { "uniref90_id": "UniRef90_UPI001F4E8A2E", "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_UPI001F4E8A2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F4E8A2E", "UniRef:UniRef50_UPI001F4E8A2E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F4E8A2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F4E8A2E" ], "product": "DUF31 family putative serine protease" } }, "id": "GIHJHMOALN_226", "locus": "GCA009939745_00187" }, { "type": "cds", "sequence": "contig_1", "start": 104591, "stop": 105115, "strand": "+", "frame": 2, "gene": null, "product": "DUF31 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AB22BFD", "UniRef:UniRef90_UPI000AB22BFD" ], "nt": "GTGATTGAAGTACCGTTAGTACTATCCAACCCCAATGATGCCAAGATCTACCAAGAGTGGATTAGACCAGCTACCCAAGCCTACAAATATTTAGGTGATGTAGAAGGTTTGTTTGCTAAGAAGGGCTATCGCAGTTACATCCAAGATTTCTACCACTTATTGGGTTATCCGGTAACTAAGAACACTAAATCAGAGTTTATTTTGGGACAAAGTCAAGGTACTGTGAACCACATGAGTTTTAGTAATGATGAGTCCAATACTACTAATACTATTACTAAAGCTAGCTTAACTCAGCAACCCCACAAGGAACAATCAGCTTACGTGGTACGTGAAAGTGGACTGCCAACCCTAACAATGAATGTGGACAAGTACACTGGTGCAAAGGGAACTCACCTAGTTAACGTTGACCAAATTACTGACCTTAGTTTGGGTGATGGTTTAATTGACTTTGGTGGATTATCACGCTTTATCTTGCAGTACCACAATGTGAACTATAAGCAATTTGGTTATGGCACGATCTTGTGA", "aa": "MIEVPLVLSNPNDAKIYQEWIRPATQAYKYLGDVEGLFAKKGYRSYIQDFYHLLGYPVTKNTKSEFILGQSQGTVNHMSFSNDESNTTNTITKASLTQQPHKEQSAYVVRESGLPTLTMNVDKYTGAKGTHLVNVDQITDLSLGDGLIDFGGLSRFILQYHNVNYKQFGYGTIL", "aa_hexdigest": "3cb5f5faca0d6a4e7f2e23a9e0102508", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 104293, "stop": 105418, "observations": { "insertion": [ 104317 ], "stop": [ 104525, 105113 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AB22BFD", "query_cov": 1.0, "subject_cov": 0.4496124031007752, "identity": 0.9940000000000001, "score": 349.0, "evalue": 1.4e-118, "gene_start": 1, "gene_end": 174, "reference_start": 101, "reference_end": 274, "reference_sequence": "MGSTISLPATQSFLLGKYTKPNVQLVKTNYEKPEDAVIEQKLKEDSLLFIQTSTLPKTAYAAIDPVNFSYNPTRTNGFWTAGKYNVYNGGNSIGNYADFAVIEVPLVLSNPNDAKIYQEWIRPATQAYKYLGDVEGLFAKKGYRSYIQDFYHLLGYPVTKNTKSEFILGQSQGTVNHMSFSNDESNTTNTITKASLTQQPHKEQSAYVVRESGLPTLTMNVDKYTGAKGTHLVNVDQITDLSLGDGLIDFGGLSRFILQYHNVNYKQFGYGTILWDTNFAGGSSGSAIFNQNKQINSIYFGALVNVTTDRNENVGLGLGQILRAPNTFNSSHEVPYSYDLIFGDVNTTNFYAQFAKKHNTHMWSKIQSTQNGEIGFHKNSKTGQQRH" }, "paralog": false, "identity": 0.9919786096256684, "score": 739.0, "evalue": 1.05e-274, "description": "Internal stop codon at 104525, 105113. Frameshift due to insertion around 104317.", "psc": { "uniref90_id": "UniRef90_UPI000AB22BFD", "product": "DUF31 family protein", "uniref50_id": "UniRef50_UPI000AB22BFD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AB22BFD", "UniRef:UniRef50_UPI000AB22BFD" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AB22BFD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AB22BFD" ], "product": "DUF31 family protein" } }, "id": "GIHJHMOALN_227", "locus": "GCA009939745_00188" }, { "type": "cds", "sequence": "contig_1", "start": 105230, "stop": 105454, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTTTAGGCTTAGGGCAAATTCTCCGTGCTCCCAATACCTTCAACTCTTCCCACGAAGTGCCCTATTCTTATGACCTCATCTTTGGTGATGTTAACACCACCAATTTTTACGCCCAGTTTGCGAAAAAGCACAACACCCACATGTGGAGCAAAATTCAAAGTACGCAGAATGGAGAAATTGGTTTCCACAAAAATTCAAAAACCGGTCAACAGCGGCATTAA", "aa": "MGLGLGQILRAPNTFNSSHEVPYSYDLIFGDVNTTNFYAQFAKKHNTHMWSKIQSTQNGEIGFHKNSKTGQQRH", "aa_hexdigest": "e9707c9fa9ae8863d20c8fdbe5f5697e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8426.264599999999, "isoelectric_point": 9.522254371643069 }, "id": "GIHJHMOALN_228", "locus": "GCA009939745_00189" }, { "type": "cds", "sequence": "contig_1", "start": 105504, "stop": 106046, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGTTACTCCGCTCTGTTTGGTATAAGCTTGGTAGTCTGTTAATCATCTTACCGCTAACGGGTTGTGGTTATGTCAGACTAAAGCGTGCTAATTTCCAAACAGACTTTACCATTAACCGCATTCCCACCCAAGGGGATGTGTACCATGATAACTATGACCTGACTTTTTCACTCAACTTTGCTACTAGTTCCAAAGATTCTTATGGTACGGGTTGGTTAATTGACTGGAAGGGTGATGAGAATAAGCCTAGCAATACCGATCCCTTCCTGATTTACTTAGCGACTAACCTACACGTAGTCGATGCCTTGCGCAATCCCCAAGACTATGAACCTTACAATAAGGATAGTAATGGTCAGGACTATGGTAATCGTGATATAACCCATTTCTTTGCATTGGGTAAATACACTGATCCAGGTTTATTGGGTGTTGAAACAAAAGAGCAATCAGCCTTTATTTCGATTCAAACATCAGCAATCCCCAAAACCGCTTATACGGCTAATGATTTTGTTGATTATCAGTATGATAGTCTTACAAAACAGTGA", "aa": "MLLRSVWYKLGSLLIILPLTGCGYVRLKRANFQTDFTINRIPTQGDVYHDNYDLTFSLNFATSSKDSYGTGWLIDWKGDENKPSNTDPFLIYLATNLHVVDALRNPQDYEPYNKDSNGQDYGNRDITHFFALGKYTDPGLLGVETKEQSAFISIQTSAIPKTAYTANDFVDYQYDSLTKQ", "aa_hexdigest": "adadb7b863850d210c0b140423272ece", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 148, "aa_cov": 0.7277777777777777, "hmm_cov": 0.9391891891891891, "evalue": 5.95143436285073e-37, "score": 123.53508758544922, "start": 46, "stop": 176 } ], "seq_stats": { "molecular_weight": 20493.577099999988, "isoelectric_point": 5.059659385681152 }, "id": "GIHJHMOALN_229", "locus": "GCA009939745_00190" }, { "type": "cds", "sequence": "contig_1", "start": 106146, "stop": 107078, "strand": "+", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_084", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47314", "UniRef:UniRef90_P75609" ], "nt": "GTGCCATTGTTCTTAGACAACGTCCGTGACAAACAGGTCTTTGATTATTTTGTCAAACCAGCCATTGAGACCTATAAAAAGCTTGGTGATACGGCTCAATTATTTACTGAGCAAAACCTAAAGGAATTGGAAAAGGAAACCTATATTATGCTGGGCTATCCGGTAGTGGAGAGCAATATTTATGCCCACATTTTAGGTCAAGGAAAAGAGTTGCGTGTGACTCAACAAGTCAACAACCAACCAGTTAAAAAGAACTATGTTTTGCAAACCATTACTAAAGAGCAACACACTTTGGATATTACGCGTGAAATACCAACCTTAATCCAAAACAAGAGTTTATCGGGTGATTTTGTTGGTAGTAAGCTCTTATCTCAAGAAGAACAACAGCAAGAACATGCTTTTTTGGGTAACTTAAACCAAGGGATAATTGACTTTGCTAGATTGTCAAACTTTAACTTGCAGTACCACAACCGCGAATACCAACAATACGGTAAGGGTTTAGCATTAGCTAACACTAACTTTAGTGGTGGTTCATCCGGTACCTTAGTTTTAAATCAACAAAAACAAATTTCCGGGGTTTACTTTGGCGTGTTAGAGTTTGGTGGCACCAACGGCACTAACCGTGAAAGTAGTATTGGTGTTGGTCAAATTTTGCGAGTAAAAGATGACGCTCAACTCCAACAACACAGTCACAACAACTTACTTTCACAATTAGGTTCCAGTCATAACTCGATTACTTACGACATTATTTTTGGTAACAAAGACACTAAAAACTACTACGCCCAGTTTGCCAAAAAACATCAAACACATTTGTACAGCCAAATAAGTTCAAGTAATCAGCAAGAACTCAAATACGTCGATAACGATCCAAATCTCAAGATTAAAGAAGAGGCTGCCAAAAGCACTGTTCAGAATTTAACTGTCTACAGTTAA", "aa": "MPLFLDNVRDKQVFDYFVKPAIETYKKLGDTAQLFTEQNLKELEKETYIMLGYPVVESNIYAHILGQGKELRVTQQVNNQPVKKNYVLQTITKEQHTLDITREIPTLIQNKSLSGDFVGSKLLSQEEQQQEHAFLGNLNQGIIDFARLSNFNLQYHNREYQQYGKGLALANTNFSGGSSGTLVLNQQKQISGVYFGVLEFGGTNGTNRESSIGVGQILRVKDDAQLQQHSHNNLLSQLGSSHNSITYDIIFGNKDTKNYYAQFAKKHQTHLYSQISSSNQQELKYVDNDPNLKIKEEAAKSTVQNLTVYS", "aa_hexdigest": "0b1614891baa0c2f003b77c7375ee4f0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 105846, "stop": 107078, "observations": { "mutation": [ 106044, 106104 ], "stop": [ 106044, 106104 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75609", "query_cov": 1.0, "subject_cov": 0.5893536121673004, "identity": 0.997, "score": 600.0, "evalue": 2.31e-213, "gene_start": 1, "gene_end": 310, "reference_start": 217, "reference_end": 526, "reference_sequence": "MPCLLRSVWYKLGSLLIILPLTGCGYVRLKRANFQTDFTINRIPTQGDVYHDNYDLTFSLNFATSSKDSYGTGWLIDWKGDENKPSNTDPFLIYLATNLHVVDALRNPQDYEPYNKDSNGQDYGNRDITHFFALGKYTDPGLLGVETKEQSAFISIQTSAIPKTAYTANDFVDYQYDSLTKQWNKKSDQKDQTQEQTVTGQSWSYKPAYADFAVIEVPLFLDNVRDKQVFDYFVKPAIETYKKLGDTAQLFTEQNLKELEKETYIMLGYPVVESNIYAHILGQGKELRVTQQVNNQPVKKNYVLQTITKEQHTLDITREIPTLIQNKSLSGDFVGSKLLSQEEQQQEHAFLGNLNQGIIDFARLSNFNLQYHNREYQQYGKGLALANTNFSGGSSGTLVLNQQKQISGVYFGVLEFGGTNGTNRESSIGVGQILRVKDDAQLQQHSHNNLLSQLGSSHNSITYDIIFGNKDTKNYYAQFAKKHQTHLYSQISSSNQQELKYVDNDPNLKIKEEAAKSTVQNLTVYS" }, "paralog": false, "identity": 0.9926829268292683, "score": 807.0, "evalue": 3.31e-297, "description": "Internal stop codon at 106044, 106104. Nonsense mutation around 106044, 106104.", "psc": { "uniref90_id": "UniRef90_P75609", "product": "Uncharacterized lipoprotein MPN_084", "uniref50_id": "UniRef50_P47314", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75609", "UniRef:UniRef50_P47314", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47314", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47314" ], "product": "Uncharacterized lipoprotein MG068" } }, "id": "GIHJHMOALN_230", "locus": "GCA009939745_00191" }, { "type": "cds", "sequence": "contig_1", "start": 108160, "stop": 108483, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTAATATTAATATGGGAGAGCAATCATCCAAAAACATGAATTCTTTTGAAAATCATGAATCAAATGAACGAAATAGTCTTCAAATTTATCAACAACATTCATCTATGATGTCAGAAACAAAGAGAAAACAAAGTAGCTTAAGTTATGATGCCAGAATTCTTTTACCTAAAAGTCCCTATAACACAAAGAAAACTTTATTTTTAATTATTTTCTTTTCCATTATCTCCTTGATATTGGCAACTATAGGTAGTGTGTTCATTTCTTTTGCTATAGTTCAAATTTCTATACCTTTTTACGTTATAGGTGGAGTTATTTGA", "aa": "MNININMGEQSSKNMNSFENHESNERNSLQIYQQHSSMMSETKRKQSSLSYDARILLPKSPYNTKKTLFLIIFFSIISLILATIGSVFISFAIVQISIPFYVIGGVI", "aa_hexdigest": "f546d3e2fc5d59c17b13b5b9171c7e6a", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12154.950799999988, "isoelectric_point": 9.397250175476074 }, "id": "GIHJHMOALN_231", "locus": "GCA009939745_00192" }, { "type": "cds", "sequence": "contig_1", "start": 108767, "stop": 108976, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_086", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75607", "UniRef:UniRef90_P75607" ], "nt": "ATGGAGTCTGCACTAAACCAAGAGTTTCAAATTGATTTTTGTGTAAAAAACAAAAAACTGTTAAAAATTTTAGCTAACGTTCTTATTGCTTCTTGGTTATCGTTTGTTGTTTTTTTAATCCTAGGTTGTATAGCTATTGATCTTTTTCGATTTGATCTTTATTCCCAATTTTTCTTTAATCACTTAAGTACTTTATCTGCTTTAGCATGA", "aa": "MESALNQEFQIDFCVKNKKLLKILANVLIASWLSFVVFLILGCIAIDLFRFDLYSQFFFNHLSTLSALA", "aa_hexdigest": "1343b7ae5fddda2403c543ec02b2302e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 108767, "stop": 109084, "observations": { "mutation": [ 108974 ], "stop": [ 108974 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75607", "query_cov": 1.0, "subject_cov": 0.6571428571428571, "identity": 1.0, "score": 131.0, "evalue": 1.77e-38, "gene_start": 1, "gene_end": 69, "reference_start": 1, "reference_end": 69, "reference_sequence": "MESALNQEFQIDFCVKNKKLLKILANVLIASWLSFVVFLILGCIAIDLFRFDLYSQFFFNHLSTLSALAWTFFVLAILFGAATLAINGFFYKEIRKKSAFKEDSK" }, "paralog": false, "identity": 0.9904761904761905, "score": 202.0, "evalue": 5.91e-69, "description": "Internal stop codon at 108974. Nonsense mutation around 108974.", "psc": { "uniref90_id": "UniRef90_P75607", "product": "Uncharacterized protein MPN_086", "uniref50_id": "UniRef50_P75607", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75607", "UniRef:UniRef50_P75607", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75607" ], "product": "Uncharacterized protein MPN_086" } }, "id": "GIHJHMOALN_232", "locus": "GCA009939745_00193" }, { "type": "cds", "sequence": "contig_1", "start": 109756, "stop": 110043, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532737.1", "SO:0001217", "UniParc:UPI0003669FDE", "UniRef:UniRef100_A0AAV5N6K4", "UniRef:UniRef50_A0AAV5N6K4", "UniRef:UniRef90_A0AAV5N6K4" ], "nt": "ATGGAAAAAGAAACAAAGAAAGATTTATTAAGTGAAGTGAAAAATAGCTTTCATTCTTCTAACAAAAATATTAAAAGCGAAATTATTTTGAAGAACACTTCAGAAAATAATATTTATTTAAGAAGCGATGCGAGAAGGCTTTTACCTAGAATCCCTTATAACACAAAGAGTGCATTATTATTCACCCTTTTATTTGCATTTCTATCTGGCCTTTCTTATGGTCTTGCATCTTTATGTATTAACTTCTCGTTTACTTCGCCACAAATTTTCATTGTCTTAATTTATTAG", "aa": "MEKETKKDLLSEVKNSFHSSNKNIKSEIILKNTSENNIYLRSDARRLLPRIPYNTKSALLFTLLFAFLSGLSYGLASLCINFSFTSPQIFIVLIY", "aa_hexdigest": "c31071c134f3730ab7ce49904dec96ae", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003669FDE", "ncbi_nrp_id": "WP_017532737.1", "uniref100_id": "UniRef100_A0AAV5N6K4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003669FDE", "RefSeq:WP_017532737.1", "UniRef:UniRef100_A0AAV5N6K4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N6K4", "uniref90_id": "UniRef90_A0AAV5N6K4" }, "psc": { "uniref90_id": "UniRef90_A0AAV5N6K4", "uniref50_id": "UniRef50_A0AAV5N6K4" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N6K4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N6K4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10880.54749999999, "isoelectric_point": 9.561386680603025 }, "id": "GIHJHMOALN_233", "locus": "GCA009939745_00194" }, { "type": "cds", "sequence": "contig_1", "start": 110296, "stop": 110751, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532738.1", "SO:0001217", "UniParc:UPI00037AA697", "UniRef:UniRef100_A0AAX0S4M2", "UniRef:UniRef50_A0AAX0S4M2", "UniRef:UniRef90_A0AAX0S4M2" ], "nt": "GTGAAACAGAATTTAAGTAGTAGAGACTTTATTAAAAAGTCAGAAAAAACAGTATCAACTACTGTAAATATAAATACAGAAGCTAGTAGTTATATTGCCAAAAATCTAGAAAACCAAAAGGCAGTTCTTTCAGCAAAGGAATTAGAAAAACAAGAGCTTTTACTAGTGCGCTTAAATGCAGTAAAAAGATTTCCGTGCTATCCATACAATACAAAAAAGGCATTTAATATTTCTGTTTTATTTGTTCTATTAGCTGGTCTATTTTTTACTGCGTCTTATTTCTCTATTTTTTATTCGATTTTTACTAGGTTTAAAGCTTTACTTATTTTCTTAGGAGTAGTTTTTATTCTTATAACTTTCATTATGCTAATTCATTGCTATATATGGTCAGTAAGGTTCAAATCAGAGTACCTTGCTAATCGTATTTATGCACTGAATTTTCAAAAAGGAGCCTAA", "aa": "MKQNLSSRDFIKKSEKTVSTTVNINTEASSYIAKNLENQKAVLSAKELEKQELLLVRLNAVKRFPCYPYNTKKAFNISVLFVLLAGLFFTASYFSIFYSIFTRFKALLIFLGVVFILITFIMLIHCYIWSVRFKSEYLANRIYALNFQKGA", "aa_hexdigest": "494e3fbfd8244fffa4a5a0f0feff0929", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00037AA697", "ncbi_nrp_id": "WP_017532738.1", "uniref100_id": "UniRef100_A0AAX0S4M2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00037AA697", "RefSeq:WP_017532738.1", "UniRef:UniRef100_A0AAX0S4M2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S4M2", "uniref90_id": "UniRef90_A0AAX0S4M2" }, "psc": { "uniref90_id": "UniRef90_A0AAX0S4M2", "uniref50_id": "UniRef50_A0AAX0S4M2" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0S4M2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S4M2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17498.497899999988, "isoelectric_point": 9.856780815124512 }, "id": "GIHJHMOALN_234", "locus": "GCA009939745_00195" }, { "type": "cds", "sequence": "contig_1", "start": 110801, "stop": 111028, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_088", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64A6", "UniRef:UniRef100_P75605", "UniRef:UniRef50_P75605", "UniRef:UniRef90_P75605" ], "nt": "ATGATAGAAATAAATAATTTGACCGCTTTAGAAAGGGATGAAAAAGTAGAAACGGAAGACATTAATACCAATGTAATAGCAAATACTTATACAGTGCAAGCCAATGTTCCAACTGCATTTCAAACCAGTGAAACAAATAGTAATACTGTTACTAACGTCCCAAAATCTCCAGAGTTAATAAGTATTCAAAGATTAGAAAGATTAAAAAATAGGTATACTTTCCTATAA", "aa": "MIEINNLTALERDEKVETEDINTNVIANTYTVQANVPTAFQTSETNSNTVTNVPKSPELISIQRLERLKNRYTFL", "aa_hexdigest": "63509dd9769b595e72105a39ef4b8b97", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64A6", "uniref100_id": "UniRef100_P75605", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64A6", "UniRef:UniRef100_P75605" ] }, "ips": { "uniref100_id": "UniRef100_P75605", "uniref90_id": "UniRef90_P75605" }, "psc": { "uniref90_id": "UniRef90_P75605", "product": "Uncharacterized protein MPN_088", "uniref50_id": "UniRef50_P75605" }, "pscc": { "uniref50_id": "UniRef50_P75605", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75605" ], "product": "Uncharacterized protein MPN_088" }, "genes": [], "id": "GIHJHMOALN_235", "locus": "GCA009939745_00196" }, { "type": "cds", "sequence": "contig_1", "start": 111388, "stop": 111567, "strand": "+", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD5284", "UniRef:UniRef90_UPI0019169453" ], "nt": "GTGGAGATTAAAACTTACAAAATTAAAGATATTTGTGAAGCTTCACATGGAAGAGAAATAAATACTAAATATCTCCGTGAAAATCAAGGAATTTATCCTGTTTATTCATCAGCAACTTCTAATGAAGGTGAAATGGGCAGAATCAAAACTTACGATTTTGATGGTGAATATGTTACCTGA", "aa": "MEIKTYKIKDICEASHGREINTKYLRENQGIYPVYSSATSNEGEMGRIKTYDFDGEYVT", "aa_hexdigest": "fad4a179cb312d5d5e4ee0fcbc69cd9b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 111388, "stop": 111624, "observations": { "mutation": [ 111565, 111577 ], "stop": [ 111565, 111577 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019169453", "query_cov": 1.0, "subject_cov": 0.7468354430379747, "identity": 1.0, "score": 127.0, "evalue": 3.64e-37, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MEIKTYKIKDICEASHGREINTKYLRENQGIYPVYSSATSNEGEMGRIKTYDFDGEYVTWTTRWSYAGSIYYRNGKFSG" }, "paralog": false, "identity": 0.9615384615384616, "score": 155.0, "evalue": 1.11e-50, "description": "Internal stop codon at 111565, 111577. Nonsense mutation around 111565, 111577.", "psc": { "uniref90_id": "UniRef90_UPI0019169453", "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_UPI0034DD5284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019169453", "UniRef:UniRef50_UPI0034DD5284" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034DD5284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD5284" ], "product": "restriction endonuclease subunit S" } }, "id": "GIHJHMOALN_236", "locus": "GCA009939745_00197" }, { "type": "cds", "sequence": "contig_1", "start": 111509, "stop": 111646, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGTGAAATGGGCAGAATCAAAACTTACGATTTTGATGGTGAATATGTTACCTGAACTACACGTTGAAGTTATGCCGGTAGCATTTACTATCGAAATGGTAAATTTAGTGCTTCCTCAAATTGTGGAATTTTAA", "aa": "MKVKWAESKLTILMVNMLPELHVEVMPVAFTIEMVNLVLPQIVEF", "aa_hexdigest": "8d282147aec9d86659585dac6a39468c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5212.366299999999, "isoelectric_point": 5.012824058532714 }, "id": "GIHJHMOALN_237", "locus": "GCA009939745_00198" }, { "type": "cds", "sequence": "contig_1", "start": 112054, "stop": 112446, "strand": "+", "frame": 1, "gene": null, "product": "Putative type I specificity subunit S-MpnORF289P C-terminus", "db_xrefs": [ "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75487", "UniRef:UniRef90_P75487" ], "nt": "TTGGTTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGCCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTACCTAAAGAAGAATTTGATATAAGGTTTTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGCTCTGCAACAGGCCAGTTAACAGTGGCACAATTTAAGGAGTATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCGCTGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTATTCCTGCTGAAATTGAACTGTGCAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGA", "aa": "MVYSKTFRVEEKSITVSARGTIGAVFYRDFAYLPAVSLICFVPKEEFDIRFLFHALRAIKFKKQGSATGQLTVAQFKEYGIHVPSLKKQKEIAAILDPLYSFFTDLNEGIPAEIELCKKQLDYYQNFLFN", "aa_hexdigest": "978c860a9603f0d19093b37a587f3b67", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75487", "query_cov": 1.0, "subject_cov": 0.896551724137931, "identity": 0.977, "score": 254.0, "evalue": 2.61e-85, "valid": true, "product": "Putative type I specificity subunit S.MpnORF289P C-terminus", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75487", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75487", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75487" ], "product": "Putative type I specificity subunit S.MpnORF289P C-terminus" }, "genes": [], "id": "GIHJHMOALN_238", "locus": "GCA009939745_00199" }, { "type": "cds", "sequence": "contig_1", "start": 113608, "stop": 113730, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7C9", "UniRef:UniRef90_A0AB38W7C9" ], "nt": "ATGGGCGGCGCGCACAACATCCAGCCCCCCATTGAGCGGTGTCGTAAAGGGCGTGTAGGGGGTGAGGGTGTTGTGGTCTTGGAGGAGGTAGGTGTTGGAAACTATTTATTAATTTTTAAGTAA", "aa": "MGGAHNIQPPIERCRKGRVGGEGVVVLEEVGVGNYLLIFK", "aa_hexdigest": "f7368d3308c1261e8c6603226c024eca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W7C9", "query_cov": 0.925, "subject_cov": 0.925, "identity": 0.919, "score": 73.2, "evalue": 1.43e-16, "valid": true, "uniref50_id": "UniRef50_A0AB38W7C9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7C9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7C9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4292.982899999999, "isoelectric_point": 7.9588960647583 }, "id": "GIHJHMOALN_239", "locus": "GCA009939745_00200" }, { "type": "cds", "sequence": "contig_1", "start": 113764, "stop": 114222, "strand": "+", "frame": 1, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_A0AB38W808" ], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGA", "aa": "MGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESG", "aa_hexdigest": "624c797a5e6239e80827d7a7c87242db", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 113716, "stop": 114429, "observations": { "mutation": [ 113758, 114220, 114328 ], "stop": [ 113758, 114220, 114328 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W808", "query_cov": 1.0, "subject_cov": 0.6413502109704642, "identity": 0.993, "score": 288.0, "evalue": 2.83e-97, "gene_start": 1, "gene_end": 152, "reference_start": 17, "reference_end": 168, "reference_sequence": "MRAAHLHTSYELVDWKVGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESGWDNQRSQKNLTNNPGPKAVTGFKLDKGRAYRKLNESWPVSNPSIRPRLARGKVPQNEKRRRQMLRVMLP" }, "paralog": false, "identity": 0.9831223628691983, "score": 455.0, "evalue": 2.26e-165, "description": "Internal stop codon at 113758, 114220, 114328. Nonsense mutation around 113758, 114220, 114328.", "psc": { "uniref90_id": "UniRef90_A0AB38W808", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W808", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "GIHJHMOALN_240", "locus": "GCA009939745_00201" }, { "type": "cds", "sequence": "contig_1", "start": 114444, "stop": 114641, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGGCTGGAAGTGCTTTTGGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAGACCGCTCTTGAAGCACACCGCCCAAATTAATTTAAGGCAAACCCAAGACAACGCGCAAACTGGTAAATTCTCCAAATACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MGAGSAFGLQGNGSNSSGLRPLLKHTAQINLRQTQDNAQTGKFSKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "9672aa81bacb828e10d4ef5ef14e6332", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6855.643300000002, "isoelectric_point": 9.992099952697757 }, "id": "GIHJHMOALN_241", "locus": "GCA009939745_00202" }, { "type": "cds", "sequence": "contig_1", "start": 114682, "stop": 114882, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "GTGGTGTTTCAGTCCAAGCAGCGACTCGGCTCGACTTCTACAAATGAAGATCTGCCGAATGTAATAACAAAGTTATACCACACCTCCACGCCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTTGTATTGGGTTGTCGGGGAGGACCAGGAATCGGGTCGCGCAACCTGA", "aa": "MVFQSKQRLGSTSTNEDLPNVITKLYHTSTPQLAYLNGQIVVMGSNAVPSLLYWVVGEDQESGRAT", "aa_hexdigest": "2b2256ab2563e2e90e9a3d3f31f045fc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 24, "aa_cov": 0.36363636363636365, "hmm_cov": 1.0, "evalue": 3.8153132430460915e-09, "score": 31.684894561767578, "start": 43, "stop": 66 } ], "seq_stats": { "molecular_weight": 7242.074800000001, "isoelectric_point": 5.53852787017822 }, "id": "GIHJHMOALN_242", "locus": "GCA009939745_00203" }, { "type": "cds", "sequence": "contig_1", "start": 114943, "stop": 115260, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F374488" ], "nt": "TTGGGTTTTAAAGATGACTCCAATTCGGATTCCAAGAATTCGAATTTGAAGGCTCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGAGCACGGAAAACAGTGCTGCCACCAAAGACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGCTCAACGGTCCGATTAATGGCTTGTTTACCCTGCTCGACAGCTTTGCCTATGTGA", "aa": "MGFKDDSNSDSKNSNLKAQGLTQPAYLIAGLDVVADHLVFAAFKAGAVGYDMSTENSAATKDQALAWSTTAGLDSAGGYKALVENTAGSTVRLMACLPCSTALPM", "aa_hexdigest": "117a739795def962c550dafa0f8bec37", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F3174D6", "query_cov": 0.8380952380952381, "subject_cov": 0.8712871287128713, "identity": 0.8859999999999999, "score": 151.0, "evalue": 1.16e-45, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI001F374488" }, "pscc": { "uniref50_id": "UniRef50_UPI001F374488", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F374488" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "GIHJHMOALN_243", "locus": "GCA009939745_00204" }, { "type": "cds", "sequence": "contig_1", "start": 115227, "stop": 115580, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0", "UniRef:UniRef90_UPI001F305329" ], "nt": "TTGTTTACCCTGCTCGACAGCTTTGCCTATGTGACCCCGGTGAGTGGCATGAAAGGGGGTAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGCGCGCTTGCCGTCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAATCAACTTCCGACGCTAATTCGAACACCAAAGTCAAGTGA", "aa": "MFTLLDSFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLNEARLPSRTDQLLVYGIVNESELKSARENAQSTSDANSNTKVK", "aa_hexdigest": "201987779b49ffe3547990682ad1dc22", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F305329", "query_cov": 0.8803418803418803, "subject_cov": 0.9035087719298246, "identity": 0.961, "score": 194.0, "evalue": 2.38e-62, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_A0AB38W8G0" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "GIHJHMOALN_244", "locus": "GCA009939745_00205" }, { "type": "cds", "sequence": "contig_1", "start": 116314, "stop": 116568, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAACACAGAATACGTAACGCGTAAAGAGTTTAATGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATAAGAATCCAGGGTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGAAAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGTTTGAACAAGATGGATGTTCGTCTAGATAAACTAGAGTCTAAGTAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLTKIETTLAAQGEQIRIQGEQIKELQIEQKAQGKTLKLILQTLQKMSDRLNKMDVRLDKLESK", "aa_hexdigest": "e9c72dd93cd27b9025b69c313a390df8", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 88, "aa_cov": 0.9642857142857143, "hmm_cov": 1.0, "evalue": 1.368048525687911e-26, "score": 88.55352783203125, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 9913.243099999994, "isoelectric_point": 8.924567985534669 }, "id": "GIHJHMOALN_245", "locus": "GCA009939745_00206" }, { "type": "cds", "sequence": "contig_1", "start": 116776, "stop": 117024, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0A0H3DJX7" ], "nt": "TTGAAAGTTTTATTTACTTTCCAAACCTTACAAAGAAAGGAGGATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCGAAGAAAAGCTTAAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTACTGATAACGAAGTAAAAAGCGAGGAAAAGCCTTTAGGCACTTGA", "aa": "MKVLFTFQTLQRKEDLNMKKVKALRNLFLEKAEEKLKYPKIASMVAYFDPKEDGTCEFIMKIKTVNEVTDNEVKSEEKPLGT", "aa_hexdigest": "bf2e33f63321979240f6528f7b20ff2f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0H3DJX7", "query_cov": 1.0, "subject_cov": 0.9647058823529412, "identity": 1.0, "score": 156.0, "evalue": 2.89e-48, "valid": true, "uniref50_id": "UniRef50_A0A0H3DJX7" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9589.156999999996, "isoelectric_point": 8.663857841491701 }, "id": "GIHJHMOALN_246", "locus": "GCA009939745_00207" }, { "type": "cds", "sequence": "contig_1", "start": 117086, "stop": 117388, "strand": "+", "frame": 2, "gene": null, "product": "Adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75267", "UniRef:UniRef90_A0A0H3DN54" ], "nt": "TTGCTGTTGGTATTTTTATTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGACTGTTTTGGACCAGGTGTTGGATTATGTTCCCTGGATTGGGAATGGGTACAGGTATGGGAATGACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAACGTCCAAGCGACGCTCGGGGGCAGTCAGACGACGACTACCAAGGGTTCGCCTTGA", "aa": "MLLVFLFSPTLSRFSTGFNLAGTVLDQVLDYVPWIGNGYRYGNDHRGSNSSTSGVTTQGQSQNASSNEPAPTFSNVGVGLKANVQATLGGSQTTTTKGSP", "aa_hexdigest": "47290fd7a38e5beb455e8da216e2716a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 117086, "stop": 117691, "observations": { "mutation": [ 117386, 117419, 117434, 117683 ], "stop": [ 117386, 117419, 117434, 117683 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A0H3DN54", "query_cov": 1.0, "subject_cov": 0.4291845493562232, "identity": 1.0, "score": 192.0, "evalue": 3.05e-60, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MLLVFLFSPTLSRFSTGFNLAGTVLDQVLDYVPWIGNGYRYGNDHRGSNSSTSGVTTQGQSQNASSNEPAPTFSNVGVGLKANVQATLGGSQTTTTKGSPWRTLDPANLQLWTGAGWRNDKASNKQSDENHTTFKSATGMGQQGQSGTSAGNPDALKQDKITKENSGNLKTEDGTATNSTTAFASNYTNLPPTADWPNALSFTNKNNAQRAQLFLRGLLGSIPVLVNKSGENN" }, "paralog": false, "identity": 0.9552238805970149, "score": 377.0, "evalue": 3e-135, "description": "Internal stop codon at 117386, 117419, 117434, 117683. Nonsense mutation around 117386, 117419, 117434, 117683.", "psc": { "uniref90_id": "UniRef90_A0A0H3DN54", "product": "Adhesin P1", "uniref50_id": "UniRef50_P75267", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A0H3DN54", "UniRef:UniRef50_P75267" ] }, "pscc": { "uniref50_id": "UniRef50_P75267", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75267" ], "product": "Putative adhesin P1-like protein MPN_131" } }, "id": "GIHJHMOALN_247", "locus": "GCA009939745_00208" }, { "type": "cds", "sequence": "contig_1", "start": 117503, "stop": 117685, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCCGATGCGTTAAAGCAGGATAAAATCACCAAGGAAAACTCGGGCAATCTCAAAACGGAGGATGGTACTGCTACTAATTCGACGACGGCATTCGCGTCGAATTACACCAACCTCCCCCCCAACCTCCCCCCCACCGCTGATTGA", "aa": "MGQQGQSGTSAGNPDALKQDKITKENSGNLKTEDGTATNSTTAFASNYTNLPPNLPPTAD", "aa_hexdigest": "07173cece760e85708463c27f1a972b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6167.520900000003, "isoelectric_point": 4.654795265197754 }, "id": "GIHJHMOALN_248", "locus": "GCA009939745_00209" }, { "type": "rRNA", "sequence": "contig_1", "start": 118195, "stop": 119710, "strand": "+", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9889106327462491, "score": 1446.2, "evalue": 2.1e-44, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGAAAGTAGTAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAGACTAGCTAATACCGCATAAGAACTTTGGTTCGCATGAATCAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTGGGGTAACGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTAAGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTTTAGCAGGTAATGGCTAGAGTTTGACTGTACCATTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATTAATCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGGGCGATCCCCTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTCTAGCGAGACTGCTAATGCAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTCGCCAGCTTGTAAAAGTGAGCAAATCTGTAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGTCGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCATTAGGAAGCGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTT", "id": "GIHJHMOALN_38", "locus": "GCA009939745_00210" }, { "type": "rRNA", "sequence": "contig_1", "start": 119938, "stop": 122843, "strand": "+", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9935042735042735, "score": 2681.3, "evalue": 8.6e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "CAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAATAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGTAGGTGGTAAGAACCTCAGATCCGGAGATTTCCGAATGGAGCAATCCGGTAGTTGGAAACAGCTATCATTAATTGATGAATAAATAGTCATATTAAAGCAATACGTGGTGAAGTGAAACATCTCAGTAGCCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACTTATCATTAGATAGGGGTTGTAGGGCTTGCAATGTGGACTTGAAAACGATAGAAGAAGCTGTTGGAAAGCAGCGCGCAAAAGGGTGATAGCCCCGTATTTGAAATTGTTTTCATACCTAGCGAGATCCCTGAGTAGCTCGGAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAAAGAGCGTTTGTTTGTTATTATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATCAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTGAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTCGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATTTATATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAAAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTTTCCACCCCCATAATTGGATTTGGGGATAAATCATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACATTGAGTGATGATGTCGAGTGAATGAGTGATCAAGTAGCGAAGGTGGTATTAATCATGCTTTCAAGAAAAGCTTCTAGGGTTAATCTAGCTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATGTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGAAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAGGACATTATCATGTAGAGAATAGGTAGGAGCAATCGATGCAAGTTCGCTAGGACTTGTTGATGCGAAAGGTGGAATACTACCCTTGGTTGTGTGCTGTTCTAATTGGTAACTGTTATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGTACAAAGGTACCTTCAGTACGGTTGGAAATCGTATGTAGAGTGTAATGGTGTAAGGGTGCTTGACTGTGAGACATACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTCCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCGTAGGAAGATTGAAGAGTGTTGCTTCTAGTACGAGAGGACCGAAGCGAGGACACCTCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCGCAAGAAAGTAAGAGCCGTCAAAGACGATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGA", "id": "GIHJHMOALN_40", "locus": "GCA009939745_00211" }, { "type": "rRNA", "sequence": "contig_1", "start": 122881, "stop": 122988, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.907563025210084, "score": 71.9, "evalue": 1.1e-11, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TTTGGTGCCCATGTCGCTGTGGAAACACCTGGTTCCATTTCGAACCCAGCAGTTAAGCACAGTGGAGCCGAATGTAGCTGTTTCAGTGAGAATAGGAAAGCACCAAGC", "id": "GIHJHMOALN_39", "locus": "GCA009939745_00212" }, { "type": "cds", "sequence": "contig_1", "start": 123139, "stop": 123438, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTACTAGTCAAAACCAAAGCCGATTTTTTCATGAACCAACAATTAAATACCACCAGGAAAAGTACAGCTGCTAGGGGCAGAATGGGACTTGTTGGTGGTATTTTACTCGTCATTGGTACCTGCATAGGGGCAGGTATTTTCTTTAAGAGTGAACGGGTGTTGCAAAACATGGGCGGAAACACCACTTTAGCCTTACTGGTGTGGTTAATGGCAGGGATTACCGTGATCTTAATGGGGTTGGCGCTGGTTGAAATTACTGCTAAAGCAGCCTTTGATGACTTGGCATTATTGTCATGA", "aa": "MVLVKTKADFFMNQQLNTTRKSTAARGRMGLVGGILLVIGTCIGAGIFFKSERVLQNMGGNTTLALLVWLMAGITVILMGLALVEITAKAAFDDLALLS", "aa_hexdigest": "79253b3f25f17ac7ef6f4756829d1455", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10516.56659999999, "isoelectric_point": 9.857876777648926 }, "id": "GIHJHMOALN_249", "locus": "GCA009939745_00213" }, { "type": "cds", "sequence": "contig_1", "start": 123754, "stop": 124062, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01490.24" ], "nt": "TTGGGTGCAAGTGCTGAACAAATGGAACACCAACCAGTGGTTCCAGTCAAAGATTTTACTGCCATTTTACCCTTCTTTGGTTGGTTTAGTGCTATGGGCGCGATTTTCTTTGCTTTTGATGGTTTTTACGTGTCAGCAGCAGCGAAAACGCAGTTGAAAAAACCAAAAAAATTACCGCAAGTAATGTTGTTGGGCTTAGGGATTGTGGTACTGATTTACAGCTTAATTGCACTAAGTGTGAGTTTAACCACACCTAACGGTGCCTTTTCGGGACTTGGTGATTGGTTAAAGCACAAAAAGCTTGGTTGA", "aa": "MGASAEQMEHQPVVPVKDFTAILPFFGWFSAMGAIFFAFDGFYVSAAAKTQLKKPKKLPQVMLLGLGIVVLIYSLIALSVSLTTPNGAFSGLGDWLKHKKLG", "aa_hexdigest": "715b5bd35c18b9c0707f2f14d30e3697", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01490.24", "name": "Aa_trans", "length": 76, "aa_cov": 0.696078431372549, "hmm_cov": 1.0, "evalue": 1.382798772637696e-07, "score": 25.98923683166504, "start": 10, "stop": 80 } ], "seq_stats": { "molecular_weight": 11002.971199999993, "isoelectric_point": 9.574667167663574 }, "id": "GIHJHMOALN_250", "locus": "GCA009939745_00214" }, { "type": "cds", "sequence": "contig_1", "start": 124222, "stop": 124668, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTTTAATCCATGCAGGGATTTTGATGGTGTTAACTACCGTAGCACTTTCTAGTTTAGGTGGTTTGTTGTACTTACCTAAAGTTAATGCTTCCTATGACGGCAAGGGCTTTAAATCAATGGGGTGCTTACTGGAGTTTGCTGACTTAATTGCCACTTGGACTAGTGTCGGGATCTTTTGGTTCTTGGGCTTAGTTTTACTGGGTGGTTTATTGCAAATCAAGAAACCGAAGCGGTGGTATTTTAGAACAACGGGTTGGCTAGCTGTAGTAGTGATTGGTCTCACTACTTTAGTGGTCATGGTGCAACCGTTTGTAGATTTAGGAATAGCAGTGTTTAACCGTAGTTATGAACGGATTGTGGCTAACACTATTTTGATTGCTATTCTTGTGATAATTGTTTTAGTAATGTTCTTTCCAACCGAACCAATTAAGCTAAGACTTTGA", "aa": "MGLIHAGILMVLTTVALSSLGGLLYLPKVNASYDGKGFKSMGCLLEFADLIATWTSVGIFWFLGLVLLGGLLQIKKPKRWYFRTTGWLAVVVIGLTTLVVMVQPFVDLGIAVFNRSYERIVANTILIAILVIIVLVMFFPTEPIKLRL", "aa_hexdigest": "e330b724742793b6c8bf951155f9565f", "start_type": "GTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16314.708199999994, "isoelectric_point": 9.77651767730713 }, "id": "GIHJHMOALN_251", "locus": "GCA009939745_00215" }, { "type": "cds", "sequence": "contig_1", "start": 124907, "stop": 125542, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTTAAGTATGGTGCGCTAATTTTTAGCGGCTTTTTAGGACTTTCAATAGTTCTAGCTAGTTGTGGGGCGAGGGGTAAATTTGATCAAGTTGATGATGGTAAGATTAAGCTAGCTTCATCTTTAACTTCTAGAAGTGCGTCAGCGGCTTTACAAAAAGTTGTTGAAAAGTACAACAAGGTTAAAGGTGTAAATGATTACCCCATTGAGATTACCCAGATTGCTGGTGGTTATGATGGCGGGCGAACTGATCTCCAAACCCGGGTTAGTGTAAAGGATAAAACCAGCTTTTACAACATGATTCTAAACTACCCTGATTTAGTGTCGGTGTTAGCCCGTAACGGGATGGAATTACCGTTTGATGGAGTCAATGTTGACAAGTTATCTCCCAACTTCTTAAAGTTCAATGAACGCATTAGTGGCGTAGCGAAAAAAGCTAACTATGCCATTCCCATTTCGATGTCAACCGATATCTTAATCCTGAATGCACCGGTGTTGCACTATATTTTGAACAGTGTTAAGAAGAATGATGGCAACACTAAAGTTCAAGTTAAAGCTCAGTCAAAAGATTCACAAACAAAAGTTAAAGGTACTATGGAAATTGGCACTGATGAAAGTACTAAAAACTTATGA", "aa": "MQFKYGALIFSGFLGLSIVLASCGARGKFDQVDDGKIKLASSLTSRSASAALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVSVKDKTSFYNMILNYPDLVSVLARNGMELPFDGVNVDKLSPNFLKFNERISGVAKKANYAIPISMSTDILILNAPVLHYILNSVKKNDGNTKVQVKAQSKDSQTKVKGTMEIGTDESTKNL", "aa_hexdigest": "8dd927650f18be9a3c50385b1f965cb6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22995.160200000017, "isoelectric_point": 9.421361351013182 }, "id": "GIHJHMOALN_252", "locus": "GCA009939745_00216" }, { "type": "cds", "sequence": "contig_1", "start": 125783, "stop": 126532, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75595", "UniRef:UniRef90_P75595" ], "nt": "TTGATCGATTTTTCAACGCGCGTAGCAAAATCATTTAAAAACAAAGTTAGTGATATTTCCACTAAAAAGGGCACTGACATTCAGGGCGTTTTAGGTCTAGACAGTACACCAAATGCTTTGTTTACCTCAGTTTTTGCTGCAGGTGATAGTAACTTTGATAACTTCTTTTATAAGGTTAAAGATGGTCGCGCTGACTTTAGTAACTTTAACGAAAACGGTACCTCTTACAAAAATCTGGAAAAGGTTTTTAATGACTATAAAAAACTCACAGACAGCAACGGTTTGTTTGTCAATAAAGGGGGTTCTTACACCTCTAACTTCCAGAAGTTTCACCAGTTAGCCTACAGCATTTCCTCCTCTTCTGGTTATGCTTATGCGTTTGCTGGTGAAAATTCAAAACGTTTAAAGTTCAACGACGATACTTTCATTGAGTACCCTAGTTTTACCCAGGAAATTCATGCGCCTGGTCAAAGTAGTCAAAAAGAAGGTGGACAGCAACAAAGCAACAGTAAAGATAACGGCAATCTTTTAGGCACTTTTACTATTGAAGCAGCGAAAAGTAAAACAAAAACAGAAGTTAAAAAAACTGAAGACACACAAAATCAAGGCAAGAAGGCTGAAGGCACACCTAACCAAGGTAAAAAAGCCGAAGGGACCGAAAACCAAGGTAAGACTATTTTCCTTTATAAAACATCAATTCCTAATGATAAGCAAGATGGTGTTGACGCTGTATTAATTAAAGACAAGTAG", "aa": "MIDFSTRVAKSFKNKVSDISTKKGTDIQGVLGLDSTPNALFTSVFAAGDSNFDNFFYKVKDGRADFSNFNENGTSYKNLEKVFNDYKKLTDSNGLFVNKGGSYTSNFQKFHQLAYSISSSSGYAYAFAGENSKRLKFNDDTFIEYPSFTQEIHAPGQSSQKEGGQQQSNSKDNGNLLGTFTIEAAKSKTKTEVKKTEDTQNQGKKAEGTPNQGKKAEGTENQGKTIFLYKTSIPNDKQDGVDAVLIKDK", "aa_hexdigest": "895fc2063a6d9c9b6b57d5f5d34dc806", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 125483, "stop": 126532, "observations": { "mutation": [ 125540, 125774 ], "stop": [ 125540, 125774 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75595", "query_cov": 1.0, "subject_cov": 0.4602587800369686, "identity": 0.996, "score": 425.0, "evalue": 3.96e-145, "gene_start": 1, "gene_end": 249, "reference_start": 293, "reference_end": 541, "reference_sequence": "MQFKYGALIFSGFLGLSIVLASCGARGKFDQVDDGKIKLASSLTSRSASAALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVSVKDKTSFYNMILNYPDLVSVLARNGMELPFDGVNVDKLSPNFLKFNERISGVAKKANYAIPISMSTDILILNAPVLHYILNSAKKNDGNTKVQVKAQSKDSQTKVKGTMEIGTDESTKNLWSDIQKKAGENGKATTEGTKKAAAKSTHLTLLTKSEQSTQGNNGASESDKKIEETWGTYSEVDGGLKNYTFKADVFDTWHGLIDFSTRVAKSFKNKVSDISTKKGTDIQGVLGLDSTPNALFTSVFAAGDSNFDNFFYKVKDGRADFSNFNENGTSYKNLEKVFNDYKKLTDSNGLFVNKGGSYTSNFQKFHQLAYSISSSSGYAYAFAGENSKRLKFNDDTFIEYPSFTQEIHAPGQSSQKEGGQQQSNSKDNGNLLGTFTIEAAKSKTKTEVKKTEDTQNQGKKAEGTPNQGKKAEGTENQGKTIFLYKTSIPNDKQDGVDAVLIKDK" }, "paralog": false, "identity": 0.994269340974212, "score": 632.0, "evalue": 8.41e-229, "description": "Internal stop codon at 125540, 125774. Nonsense mutation around 125540, 125774.", "psc": { "uniref90_id": "UniRef90_P75595", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75595", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75595", "UniRef:UniRef50_P75595", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75595" ], "product": "Uncharacterized lipoprotein MPN_097" } }, "id": "GIHJHMOALN_253", "locus": "GCA009939745_00217" }, { "type": "cds", "sequence": "contig_1", "start": 126830, "stop": 126934, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGACTCAAGGTCCTAATCTAATTGGTATCCATACTAATACCAAGGAAAATGAAGAAACGCAAAAGTTTGTTAACTGGTTCTTAAATACCAGTCTAACTTGA", "aa": "MMTQGPNLIGIHTNTKENEETQKFVNWFLNTSLT", "aa_hexdigest": "2047a8bc4f0b3da0607b140375041861", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3938.3996000000006, "isoelectric_point": 5.478164863586425 }, "id": "GIHJHMOALN_254", "locus": "GCA009939745_00218" }, { "type": "cds", "sequence": "contig_1", "start": 127124, "stop": 127273, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTTACAGTGATCCAAGCGATTTCAGGGGTGGTAAATTCCGCGAATCAATTGGTTCTGCCTTTAACGCCACGGTCAATTCACACGTTGACTTTAATACTTTCGTTGCTAACTTCCGTGCTAACTTAGGTTCTGGTTACGATAAATAG", "aa": "MGYSDPSDFRGGKFRESIGSAFNATVNSHVDFNTFVANFRANLGSGYDK", "aa_hexdigest": "f1815f7d0d0feaaebcea9c9fb1c32008", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5352.7325, "isoelectric_point": 6.516329002380371 }, "id": "GIHJHMOALN_255", "locus": "GCA009939745_00219" }, { "type": "cds", "sequence": "contig_1", "start": 127245, "stop": 127685, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0010051FB8", "UniRef:UniRef100_A0AB38W7Y3", "UniRef:UniRef50_A0AB38W7Y3", "UniRef:UniRef90_A0AB38W7Y3" ], "nt": "GTGGTTTTCGTTGCCTTAGGTGTTATTCCCAGTAGCCTTATCATTCCTTCACCCCGCCCCCGTTCAGAGCTGGAGGTTGGCTTGGTCGAGGGTGGGTCATTTGGGAATATCTTTCCCCGTAGTCGTCGTCTGACTGCCCCCGAGGGTGGCTTGGACATTCGCTTTGAGACCGACGCCGACATTCGAAAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTTAGGCGCGGTTATATCATCCACACCCCGGTGATCATTCCCATACCTGTGCCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCGAGGAGTGCCCCCGCGAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGGGATTAACTATTTATCGTAACCAGAACCTAAGTTAG", "aa": "MVFVALGVIPSSLIIPSPRPRSELEVGLVEGGSFGNIFPRSRRLTAPEGGLDIRFETDADIRKRREKGTRTTCVRTSSAGRPRAGFRRGYIIHTPVIIPIPVPIPNPGNIIQHLVEECPREVEPGGETGKRGGEVGLTIYRNQNLS", "aa_hexdigest": "ce37e5c0bd7b24764ba1e49899f0ee2a", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0010051FB8", "uniref100_id": "UniRef100_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0010051FB8", "UniRef:UniRef100_A0AB38W7Y3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7Y3", "uniref90_id": "UniRef90_A0AB38W7Y3" }, "psc": { "uniref90_id": "UniRef90_A0AB38W7Y3", "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15948.147299999986, "isoelectric_point": 10.137669563293457 }, "id": "GIHJHMOALN_256", "locus": "GCA009939745_00220" }, { "type": "cds", "sequence": "contig_1", "start": 127977, "stop": 128258, "strand": "+", "frame": 3, "gene": null, "product": "Adhesin P1-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6", "UniRef:UniRef90_A0AB33HTF6" ], "nt": "GTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCTCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCTTTGGTAGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNRSGSDSNKFQATDQKWSYTDLQSDQTKLNLSAYGEVNGLLNPALVETYFGTTRTSSTANQNSTTVPGIGFKIPEQNNDSKATLITPGLA", "aa_hexdigest": "e42bb8ca944d16dfc92afce773255570", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 127905, "stop": 128554, "observations": { "deletion": [ 128334 ], "mutation": [ 128256 ], "stop": [ 128256, 128400 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HTF6", "query_cov": 1.010752688172043, "subject_cov": 0.42727272727272725, "identity": 0.862, "score": 155.0, "evalue": 7.97e-46, "gene_start": 1, "gene_end": 93, "reference_start": 25, "reference_end": 118, "reference_sequence": "MSFTNKNNAQRVQLFLRGLLGSIPVLVNKSGQDDNSKFNSTDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTLTSSTANQNSTTVPSIGFKIPEQNNDSKAVLITPGLAWTPQDVGNLVVSGTSLSFQLGGWLVTVHGLYQTPRGLFGPPVKWPGCQWQRPKGVNLGQAALSGISWQLGQPVGTGRECVGFEGGVGGSSSQRGVRVASSYK" }, "paralog": false, "identity": 0.8454545454545455, "score": 358.0, "evalue": 2.05e-128, "description": "Internal stop codon at 128256, 128400. Frameshift due to deletion around 128334. Nonsense mutation around 128256.", "psc": { "uniref90_id": "UniRef90_A0AB33HTF6", "product": "Adhesin P1 homolog", "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HTF6", "UniRef:UniRef50_A0AB33HTF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6" ], "product": "Adhesin P1 homolog" } }, "id": "GIHJHMOALN_257", "locus": "GCA009939745_00221" }, { "type": "cds", "sequence": "contig_1", "start": 128481, "stop": 129002, "strand": "+", "frame": 3, "gene": null, "product": "Putative adhesin P1-like protein MPN_099", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593", "UniRef:UniRef90_P75593" ], "nt": "GTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTGCCACCACCACCGCAACAGGGGCTACCTTACCGGAACACCCGAATGCTTTGGCGTACCAAATTAGCTATACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCTCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTCGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTGTTTGGAGCCATGAGTGGTAACCTCGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGGCAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACCATTAATAGGAATTTTTCTTATTAA", "aa": "MESVWDLKGVWADQAQLAAQAATSSATTTATGATLPEHPNALAYQISYTDKDSYKASTQGSGQTNSQNNSLYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGAMSGNLGSVLSGGGAGGAGSTNSVDLSPVERVSGSLTINRNFSY", "aa_hexdigest": "afabdaf6bdce724eb0ef285727cd104f", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 128181, "stop": 129002, "observations": { "mutation": [ 128256, 128439 ], "stop": [ 128256, 128439 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75593", "query_cov": 1.0, "subject_cov": 0.4357682619647355, "identity": 0.9940000000000001, "score": 324.0, "evalue": 9.56e-109, "gene_start": 1, "gene_end": 173, "reference_start": 225, "reference_end": 397, "reference_sequence": "TTQDGNATGQQEATNYTNLPPNLTPTADWPNALSFTNKNNAQRAQLFLRGLLGSIPVLVNRSGSDSNKFQATDQKWSYTDLQSDQTKLNLSAYGEVNGLLNPALVETYFGTTRTSSTANQNSTTVPGIGFKIPEQNNDSKATLITPGLAWTPQDVGNLVVSGTTVSFQLGGWLVTFTDFVKPRAGYLGLQLSGLDASDSDQRELIWAPRPWAAFRGSWVNRLGRVESVWDLKGVWADQAQLAAQAATSSATTTATGATLPEHPNALAYQISYTDKDSYKASTQGSGQTNSQNNSLYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGAMSGNLGSVLSGGGAGGAGSTNSVDLSPVERVSGSLTINRNFSY" }, "paralog": false, "identity": 0.9926739926739927, "score": 539.0, "evalue": 1.59e-195, "description": "Internal stop codon at 128256, 128439. Nonsense mutation around 128256, 128439.", "psc": { "uniref90_id": "UniRef90_P75593", "product": "Putative adhesin P1-like protein MPN_099", "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75593", "UniRef:UniRef50_P75593" ] }, "pscc": { "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593" ], "product": "Putative adhesin P1-like protein MPN_099" } }, "id": "GIHJHMOALN_258", "locus": "GCA009939745_00222" }, { "type": "cds", "sequence": "contig_1", "start": 129090, "stop": 129341, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_015344874.1", "SO:0001217", "UniParc:UPI0002B8420C", "UniRef:UniRef100_A0AB38WA43", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0A0H3DNZ5" ], "nt": "ATGAAATTAGTTAAATTAACGAGGCTTCACCGCTTTTTAAAACAAGTTCAATTGCAACTTGTTCCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTATTGGCGCTTCTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCAGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAA", "aa": "MKLVKLTRLHRFLKQVQLQLVPLFSQNFLQVLLALLNKPVNLFSENTTMKTNKKYAFAALLMQAQAKLPNSFKFFLSKKGGMA", "aa_hexdigest": "18d85a11dacc9036768befe2855820f5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B8420C", "ncbi_nrp_id": "WP_015344874.1", "uniref100_id": "UniRef100_A0AB38WA43", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B8420C", "RefSeq:WP_015344874.1", "UniRef:UniRef100_A0AB38WA43" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38WA43", "uniref90_id": "UniRef90_A0A0H3DNZ5" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DNZ5", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9528.455399999993, "isoelectric_point": 10.966668510437014 }, "id": "GIHJHMOALN_259", "locus": "GCA009939745_00223" }, { "type": "cds", "sequence": "contig_1", "start": 129509, "stop": 129892, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254", "UniRef:UniRef90_P75592" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAATTTAACGAATATAAAGTTTCAAACGACCAACGGCTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAGGTTGAGCAAATTAAAGCTCAAGGAAAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCACTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAG", "aa": "MNTEYVTRKEFNEYKVSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQVEQKAQVEQIKAQGKQIKAQGKTLKSILQALGGINKRLDKIDPPK", "aa_hexdigest": "ebca61d28ac22e2cebbba92a3f60f342", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 129341, "stop": 129892, "observations": { "mutation": [ 129422 ], "stop": [ 129422 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75592", "query_cov": 1.0, "subject_cov": 0.6939890710382514, "identity": 0.89, "score": 95.5, "evalue": 2.68e-22, "gene_start": 1, "gene_end": 127, "reference_start": 57, "reference_end": 183, "reference_sequence": "MKEKIPFYNEKEFHDMVKKTKKGTFSGWYIIDKDNKSVEFSGSFNRQFKLNKPVIPVNTEYVTRKEFNEYKVSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQVEQKAQGEQIKAQGKQIKAQGKTLKSILQALGGINKRLDKIDPPK" }, "paralog": false, "identity": 0.9234972677595629, "score": 211.0, "evalue": 2.37e-70, "description": "Internal stop codon at 129422. Nonsense mutation around 129422.", "psc": { "uniref90_id": "UniRef90_P75592", "product": "UPF0134 protein MPN_100", "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75592", "UniRef:UniRef50_P75254" ] }, "pscc": { "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254" ], "product": "UPF0134 protein MPN_524" } }, "id": "GIHJHMOALN_260", "locus": "GCA009939745_00224" }, { "type": "cds", "sequence": "contig_1", "start": 130349, "stop": 130606, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGACAACAGCGCCAAGGGCATCACTGCCGGTAGTGAAAGTCAACAAACCACGTATGATCCCACTCGAACCGAATCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTAGTAGGTTAAACCCACAGACACCAACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNSAKGITAGSESQQTTYDPTRTESALTASTTFALRRYDLAGRALYDLDFSRLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "3aa39a00115a9c9bb29a4a7b736df92d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 57, "aa_cov": 0.6705882352941176, "hmm_cov": 0.8947368421052632, "evalue": 8.100973488650042e-09, "score": 30.690603256225586, "start": 28, "stop": 84 } ], "seq_stats": { "molecular_weight": 9206.930499999995, "isoelectric_point": 6.072189140319824 }, "id": "GIHJHMOALN_261", "locus": "GCA009939745_00225" }, { "type": "cds", "sequence": "contig_1", "start": 130637, "stop": 131155, "strand": "+", "frame": 2, "gene": null, "product": "Adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571", "UniRef:UniRef90_A0AAX0SQU5" ], "nt": "GTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGAACAAGGCACCGAACAGGTGGAACTTGAATCTAATGATCTCGCCAACGTTCCCATTAAGCGGGGCGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGDESKSSNGSSSTSTTTQRGGSSGDTKVKALQVAVKKKSGSQGNSGEQGTEQVELESNDLANVPIKRGEESGQSVQLKAADFGTTPSSSGSGGNSNPGSPTP", "aa_hexdigest": "14416f07bfcd6de52dccf2fbaea94ead", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "both", "pseudogene": { "start": 130349, "stop": 131458, "observations": { "mutation": [ 130604, 131153, 131201, 131315, 131330, 131354 ], "stop": [ 130604, 131153, 131201, 131315, 131330, 131354 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAX0SQU5", "query_cov": 1.0, "subject_cov": 0.4047058823529412, "identity": 0.9420000000000001, "score": 271.0, "evalue": 1.16e-87, "gene_start": 1, "gene_end": 172, "reference_start": 97, "reference_end": 268, "reference_sequence": "MRDNSAKGITAGSESQQTTYDPTRTESALTASTTFALRRYDLAGRALYDLDFSRLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKNKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGDESKSSNGSSSTSTTTQRGGSSGDTKVKALQVAVKKKSGSQGNSGEQGTEQVELESNDLANVPIKRGEESGQSVQLKAADFGTTPSSSGSGGNSNPGSPTPWRPWLATEQIHKDLPKWSASILILYDAPYARNRTVIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDQGQAVADNTQTGFDTDDTDNKKQGFQKEADSDKPAPIALPFEAYFANIGNLTWFGQALLVFGICLS" }, "paralog": false, "identity": 0.964769647696477, "score": 683.0, "evalue": 4.98e-252, "description": "Internal stop codon at 130604, 131153, 131201, 131315, 131330, 131354. Nonsense mutation around 130604, 131153, 131201, 131315, 131330, 131354.", "psc": { "uniref90_id": "UniRef90_A0AAX0SQU5", "product": "Adhesin P1", "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAX0SQU5", "UniRef:UniRef50_P75571" ] }, "pscc": { "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571" ], "product": "Uncharacterized protein MPN_205" } }, "id": "GIHJHMOALN_262", "locus": "GCA009939745_00226" }, { "type": "cds", "sequence": "contig_1", "start": 131117, "stop": 131593, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCGAACCAGGTGAGGTTGCCAATGTTGGCGAAGTACGCTTCAAACGGGAGGGCGATCGGGGCCGGCTTGTCGGAGTCAGCTTCCTTTTGAAAGCCTTGTTTTTTATTATCGGTGTCATCTGTATCAAACCCGGTTTGGGTATTATCCGCGACCGCTTGGCCCTGGTCAAACCACTCGGGGTGGCGCCGCGGGTTGAAGAACCCGGTGGTTTGGAGCAAAACATCTCTGGCCTTTCAGTCCCACAACCCGTGGTGGTTTCACTTGGGCGTTCTTCAACTGGGCGGATAGTTCGCGGTCATCACCTTGGGATCCAAGTGATCAACGCGATCAATGACGGTACGATTGCGCGCATAAGGCGCATCGTACAGAATCAGGATCGAGGCGGATCATTTGGGGAGGTCCTTGTGAATTTGCTCGGTCGCAAGCCACGGCCTTCAGGGGGTGGGGGAACCGGGGTTGGAGTTGCCGCCTGA", "aa": "MPEPGEVANVGEVRFKREGDRGRLVGVSFLLKALFFIIGVICIKPGLGIIRDRLALVKPLGVAPRVEEPGGLEQNISGLSVPQPVVVSLGRSSTGRIVRGHHLGIQVINAINDGTIARIRRIVQNQDRGGSFGEVLVNLLGRKPRPSGGGGTGVGVAA", "aa_hexdigest": "55f4d07df8dcf4ad6b3e40dce0d12851", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16565.13849999998, "isoelectric_point": 11.222479057312011 }, "id": "GIHJHMOALN_263", "locus": "GCA009939745_00227" }, { "type": "cds", "sequence": "contig_1", "start": 131769, "stop": 132206, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAGGCTTAATGATGGGACGAGTTCGGATTCCAATACCACCACTGATGGGGCGCTTACGTTTGACACGAATGAAAGTCAAAAAGCTTTAGGCTCCACGGACACGCAGGGTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGA", "aa": "MGQNKLVALVRSALVRVRLNDGTSSDSNTTTDGALTFDTNESQKALGSTDTQGSGSNSQDFASYILIFKAAPRATWVFERKIKLALPYVKNESGAGDSTTTNSGSLYKTLQDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESG", "aa_hexdigest": "fca8adc49ca3ff2021bc087765450749", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15382.876899999977, "isoelectric_point": 6.564755821228027 }, "id": "GIHJHMOALN_264", "locus": "GCA009939745_00228" }, { "type": "cds", "sequence": "contig_1", "start": 132318, "stop": 132926, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028006D2D" ], "nt": "GTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTTCTAGTACCAGTTTGGGCAATGTTGGGGACACCACTGCCCTAACTCCACTTTTAAGAGGTGCTACTACTACTACCGTCCAGCTAAAACAAACAGATTCTAACAGTCAAGATCAACAAAAATTCCAAAAATATTTAAACACCGCCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCAGAACTTAGCCAAGGCGGTTGGCAAAGCCAAACAGCGCGGCACAGTTCCACCCGGGGACTACAGTCAGCCGGGGCCAATCGTGGCGCTAGTTTAGCGGCACGCACCACCACAACTGTGGGGGTGGGTAGAAGAGATTCCACTTCCACCACCTTGGAACTACCAAACGTCATCACCCAGCTTTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGA", "aa": "MYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGSTGSQMASSTSLGNVGDTTALTPLLRGATTTTVQLKQTDSNSQDQQKFQKYLNTAQALHQMGVIVPELSQGGWQSQTARHSSTRGLQSAGANRGASLAARTTTTVGVGRRDSTSTTLELPNVITQLYHTSTSQLAYLNGQIVVMGSNAVPSLWYWVVDERTTSGRAT", "aa_hexdigest": "9ef0a791cf7feb3dfb0a34ddf039f94b", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028006D2D", "query_cov": 0.8217821782178217, "subject_cov": 0.9651162790697675, "identity": 0.59, "score": 146.0, "evalue": 3.51e-41, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI0028006D2D" }, "pscc": { "uniref50_id": "UniRef50_UPI0028006D2D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028006D2D" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "GIHJHMOALN_265", "locus": "GCA009939745_00229" }, { "type": "cds", "sequence": "contig_1", "start": 132942, "stop": 133475, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W870" ], "nt": "TTGAACTTCGGCACTGAAGTTCAAAAAAACTTCGTCGAAAACCAGTTGGGGTTTAAATCCGAAGACAACTCCAACACCTCCTTGACGAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACTTAATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGTACAACAACCTGGTGACGAATACTGCGGGGTTAAACGGTCCGATTAACGGCTTGTTTACCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATAACGAAGAAGTGCAAACGACCTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCGTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGTGGATAATGGCGATAAAAAAGCCATCCACCTAAGGTGA", "aa": "MNFGTEVQKNFVENQLGFKSEDNSNTSLTNFKSQGLTQPAYLISGLDVVADHLVFAAFKAGAVGYDMTTDSNASTYNQALTWSTTAGLDSDGGYNNLVTNTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSKNNEEVQTTYPVKSDQKATAKIASLINASPLNSYVDNGDKKAIHLR", "aa_hexdigest": "e476f124e83d58d2b0b2634383c5e0d0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W870", "query_cov": 0.9152542372881356, "subject_cov": 0.8901098901098901, "identity": 0.698, "score": 187.0, "evalue": 1.22e-57, "valid": false, "product": "MgpC protein", "uniref50_id": "UniRef50_A0AB38W870" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W870", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W870" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "GIHJHMOALN_266", "locus": "GCA009939745_00230" }, { "type": "cds", "sequence": "contig_1", "start": 133552, "stop": 133770, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00059C39D0", "UniRef:UniRef100_A0AB38W8B4", "UniRef:UniRef50_A0AB38W8B4", "UniRef:UniRef90_A0AB38W8B4" ], "nt": "ATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTACCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAGTAGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTACAACTCCATCTAACTATTAGTAGGGTTTTCACTTTTGAAAATTTTTTTGGATTAATTTTGGCACCCCGCCTTTTTTAA", "aa": "MNLLNLFKSAGAGSFFQHKSLYLLPLNKLRQIFKVAKEFEYFCNLGLQLHLTISRVFTFENFFGLILAPRLF", "aa_hexdigest": "6c7d16b8c20ece8415b2c8db7655b76c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00059C39D0", "uniref100_id": "UniRef100_A0AB38W8B4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00059C39D0", "UniRef:UniRef100_A0AB38W8B4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8B4", "uniref90_id": "UniRef90_A0AB38W8B4" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8B4", "uniref50_id": "UniRef50_A0AB38W8B4" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8B4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8415.915199999992, "isoelectric_point": 9.897847175598145 }, "id": "GIHJHMOALN_267", "locus": "GCA009939745_00231" }, { "type": "cds", "sequence": "contig_1", "start": 134008, "stop": 134583, "strand": "+", "frame": 1, "gene": null, "product": "DUF16 family-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75565", "UniRef:UniRef90_A0AB38W7Z0" ], "nt": "ATGATGAGTAAAAATAAATTCTTTACCATTACTAAAGAACAGTACAGGAAGTTTACACGCAATAAAATTGAACTTCTTTTTAAGGTCCAATGTACCGAAAAGAAAAAGGGTAAACGTAGGTTCAAAATATTTCAGCAAGTCGAGGATAAACCAAAACCACCAGTTAAAATAATTGAAAAGGAATCTAAGGGACCGGAAGAACCAAAGGATCCTGGTATTAAAAATGGACCTAAGGGTCCCGGTGGACCAAAAGGACCTAAAAAGCCACCTGTTCCCGATGATCCTGATACACCAGATGTTCCTAAAGGCCCCAAAGAACCAGGCGGTTCAAAAGGTCCAGGTGGTCCCGATGATCCTAATGGAGGAAAGAAGAAAGTGCCTAAACCAGATAAGTATGTAACCCATCGCGAGCTTGATGAGAAACTTACTGAGTTTCATGACAAGCTTATGGTAGAACTTGATGGTAAGTTTGCTACTAAAGCTGATTTAAAACGGGTTGAAGATAAAGTTGATGTTTTGTTTGAATTGCAAAAAACGCAAGATGAACAAATCAAGGTTCAAGGAAAACAAATCTAA", "aa": "MMSKNKFFTITKEQYRKFTRNKIELLFKVQCTEKKKGKRRFKIFQQVEDKPKPPVKIIEKESKGPEEPKDPGIKNGPKGPGGPKGPKKPPVPDDPDTPDVPKGPKEPGGSKGPGGPDDPNGGKKKVPKPDKYVTHRELDEKLTEFHDKLMVELDGKFATKADLKRVEDKVDVLFELQKTQDEQIKVQGKQI", "aa_hexdigest": "50833bf10d8c9adc3ac0f437fc726cad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W7Z0", "query_cov": 0.9947643979057592, "subject_cov": 1.0, "identity": 1.0, "score": 261.0, "evalue": 2.5e-86, "valid": true, "product": "DUF16 family-like protein", "uniref50_id": "UniRef50_P75565" }, "pscc": { "uniref50_id": "UniRef50_P75565", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75565" ], "product": "UPF0134 protein MPN_104" }, "genes": [], "id": "GIHJHMOALN_268", "locus": "GCA009939745_00232" }, { "type": "cds", "sequence": "contig_1", "start": 134966, "stop": 135145, "strand": "+", "frame": 2, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCY4", "UniRef:UniRef90_A0AAN5BCY4" ], "nt": "ATGAAAGATGAAACAAATATTTGTTCTCAATCTTTAAAAAGACCTTTAAGCGAAGACCAATTGATAGAGTATTTGAGTACTTATGAAATAACTTTATTTGAGGTTTATGCTGAGGATATGCTAGATGGCAATTGCAAAATTTATATACATGCTAAAGGACGTCCAAAAGACCCAGATTGA", "aa": "MKDETNICSQSLKRPLSEDQLIEYLSTYEITLFEVYAEDMLDGNCKIYIHAKGRPKDPD", "aa_hexdigest": "402782f5382e4b9b10aba387c56fea46", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 134966, "stop": 135232, "observations": { "mutation": [ 135143 ], "stop": [ 135143 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN5BCY4", "query_cov": 1.0, "subject_cov": 0.6704545454545454, "identity": 1.0, "score": 122.0, "evalue": 2.25e-35, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MKDETNICSQSLKRPLSEDQLIEYLSTYEITLFEVYAEDMLDGNCKIYIHAKGRPKDPDWKRKTDKKSLEIERYVKKFIKFLKREQKK" }, "paralog": false, "identity": 0.9886363636363636, "score": 177.0, "evalue": 3.52e-59, "description": "Internal stop codon at 135143. Nonsense mutation around 135143.", "psc": { "uniref90_id": "UniRef90_A0AAN5BCY4", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAN5BCY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN5BCY4", "UniRef:UniRef50_A0AAN5BCY4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAN5BCY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCY4" ], "product": "Family K-like protein" } }, "id": "GIHJHMOALN_269", "locus": "GCA009939745_00233" }, { "type": "cds", "sequence": "contig_1", "start": 135786, "stop": 136628, "strand": "+", "frame": 3, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "COG:COG0016", "COG:J", "EC:6.1.1.20", "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_P75564", "UniRef:UniRef90_P75564" ], "nt": "TTGGAAAGCACCTTGCAAACACTCCATGATACACAGCTACAAGTCTTTACCCAAGCCCAGTCTAGCAGTGTATTAACTCATGGTGATGTGATGTTGCTAGCAACCTCTTTTGCCCCTGGTTCTAGCAACATTATTTACCAGGTAATTGATGAGTTAGTGAACTATTTTAAGAAGTTCTTGTTTACGGTTAACTATGACAGTGAATTGACTACTATTGCAGACTGTTTTGACCTGCTAAACATTCCCAAAGACCATCCTAGTCGTAACCTTACTGACACCTTTTACTTAGACAAAAACCGCTTACTGAGAACACATTGTACTGCTGCTACTTTACGTGCTGTTAAGGAAACGAAAAAGAGTAATAACCCCGATATTCGGATTGCTTCCTTTGGTGCTGTTTTTCGTAAAGATGATGACGATGCCACCCACTCACACCAGTTCAACCAACTGGACTTCATGTGGATTAAAAAGGATTTTTCTTTAACTAACCTCAAGTGGTTTATGCAGAATATGATTAACCACATCTTTGGCGAAAATACTTCAGCGCGTTTTCGTCTGTCCCACTTCCCTTTTACCGAACCTTCCTTTGAAATTGACATCCGTTGCTGGTTGTGTCAGAATGGTTGTGGTGTATGTAAGAAAACACGTTGGATTGAGGTATTAGGGGCGGGTATCCTCCACCCTCAAGTGATGGCTAACATGGGCTTTAGTGACACGGATAATATAAGGGGTATAGCAGCTGGCATTGGGATTGAGCGGTTGGTAATGTTGAAACACGGCATTAGTGACATCCGTGACCTTTACGACAATAACTTCAAATTTTTAGCCCAGTTTACTGATTAG", "aa": "MESTLQTLHDTQLQVFTQAQSSSVLTHGDVMLLATSFAPGSSNIIYQVIDELVNYFKKFLFTVNYDSELTTIADCFDLLNIPKDHPSRNLTDTFYLDKNRLLRTHCTAATLRAVKETKKSNNPDIRIASFGAVFRKDDDDATHSHQFNQLDFMWIKKDFSLTNLKWFMQNMINHIFGENTSARFRLSHFPFTEPSFEIDIRCWLCQNGCGVCKKTRWIEVLGAGILHPQVMANMGFSDTDNIRGIAAGIGIERLVMLKHGISDIRDLYDNNFKFLAQFTD", "aa_hexdigest": "8632576b4a53936657e85c6b3d35df72", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75564", "query_cov": 1.0, "subject_cov": 0.8211143695014663, "identity": 0.996, "score": 567.0, "evalue": 1.53e-203, "valid": true, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_P75564", "cog_id": "COG0016", "cog_category": "J", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_P75564", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75564" ], "product": "Phenylalanine--tRNA ligase alpha subunit" }, "genes": [ "pheS" ], "id": "GIHJHMOALN_270", "locus": "GCA009939745_00234" }, { "type": "cds", "sequence": "contig_1", "start": 136631, "stop": 137362, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGTTAATTTCTAAAAAAACTTTAGCTGTTTTAATTCCCGAAATTACCCACGTTTCCAACAATGAAATTTGTGAAAAGTTACAGCAAATCGGGATTGAAGTGGAAGCAATTCGCGCCTTTAAAAATCCTGATTACTTACAGTTGGGTATTCTCCGCGCTGTTACCCCCCACCCCCATGACAACCACTTGTATGTGTGTCAAGTTCAAATAGACAAAAACAAACAGCTCAATGTGGTGACTGGGGCAGTTAACATAGTTGATCCCAATAATCTCAATAAACACGTGATTGTCGCCAAAAAAGGGGCAGAACTGCTCAATGGTTTAATCATTAAAACGAAGAACATTAAGGGAATTATCTCGGACGGGATGTTGTGTAGTTATGTTGATATTAATCCGTTTAGTAAGCATTTAATTGCTGACGGTGATGACACGCATGCGATTGTGCTAGACAACATTAACCGCGATGAATTTGGTGATTATTTAAGTTTTTTAAACCTCGATGACGTGGTGTTTGAGGTTACTTTACCTACCAACCGTTCTGATTTACAGAGCTTAATCTTCCTCGCTAAGGAACTAGCAGCAGTGTTGAAACGTCCGATCTTCTTGGAACAGAAAACCACCATGACCCTGCGCGAGTTCTACCGCTTTCCGCTTAACTTAAGAAACCGTGCTCAGGCCAATTTCTTTGGTGGACTTTTTTTGCGTGATGTAGCAATTACCAGTTCCCCTTGA", "aa": "MLISKKTLAVLIPEITHVSNNEICEKLQQIGIEVEAIRAFKNPDYLQLGILRAVTPHPHDNHLYVCQVQIDKNKQLNVVTGAVNIVDPNNLNKHVIVAKKGAELLNGLIIKTKNIKGIISDGMLCSYVDINPFSKHLIADGDDTHAIVLDNINRDEFGDYLSFLNLDDVVFEVTLPTNRSDLQSLIFLAKELAAVLKRPIFLEQKTTMTLREFYRFPLNLRNRAQANFFGGLFLRDVAITSSP", "aa_hexdigest": "856a218a20336e72f3cc3f8d7bcfb2ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 85, "aa_cov": 0.34156378600823045, "hmm_cov": 0.8823529411764706, "evalue": 3.219328930913425e-13, "score": 45.12896728515625, "start": 48, "stop": 130 } ], "seq_stats": { "molecular_weight": 27315.34370000002, "isoelectric_point": 6.653595161437989 }, "id": "GIHJHMOALN_271", "locus": "GCA009939745_00235" }, { "type": "cds", "sequence": "contig_1", "start": 137375, "stop": 138034, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03483.22" ], "nt": "TTGTTAATTAACCAGGAGTTACGTCCAGTCAACTGCTTTGTGGACCAGGCTAATATGGTAACTGTTTACACTGGTCAACCAATCCACTGCCATGATGCTGACAAGATCCATGGAAGTGTGGAACTTAAACTAGCCACCCAATTGGAAACGATGTTGGCCTTAGACAACAAGGAGTATGAGATTAAACCGGGTGATCTAGTGGTAGCTGATGAACAGGGCACAATTGCCATAGTCGGTATTATTGGTAGTAAGCGCACTATGGTCGACAATACGACCAACAACATCTTTTTTGAAGTTGTTAACTATAACCATGAGCGAATTAAGCAAACCGCTCAGCGTTTAGGAGTTGCTAACTTTGCTAGTAGGTTAATGAGTAAACCAATTAGTTTACAAGCTACCGAAAACTGCTTGAACTATTTGCAAAATAACTTCCTCAATCCCGAAAGCATTGGCAAGATTAGTAAGTTTTCCAGCACCATTAAAGCCCCAGCTTTTAACCGCAAAATTTACCTCAACTTTAATCAGTTACGGGAGTTAATTGGAGTGACTAAAAAGCAGTTAAATGACTACATGATCCGTAACTACCTTACTAGTTTAGGGTTCAAAATGGAAAACCAAATTGCCCGCGCCCCCGCTTACCGGCAAGACATTACGGTTTGA", "aa": "MLINQELRPVNCFVDQANMVTVYTGQPIHCHDADKIHGSVELKLATQLETMLALDNKEYEIKPGDLVVADEQGTIAIVGIIGSKRTMVDNTTNNIFFEVVNYNHERIKQTAQRLGVANFASRLMSKPISLQATENCLNYLQNNFLNPESIGKISKFSSTIKAPAFNRKIYLNFNQLRELIGVTKKQLNDYMIRNYLTSLGFKMENQIARAPAYRQDITV", "aa_hexdigest": "10ed1f56ab4a5036700e6912ac4ceb4e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03483.22", "name": "B3_4", "length": 143, "aa_cov": 0.6529680365296804, "hmm_cov": 1.0, "evalue": 5.344980857857591e-44, "score": 145.3409881591797, "start": 2, "stop": 144 } ], "seq_stats": { "molecular_weight": 24831.27690000002, "isoelectric_point": 8.692675209045408 }, "id": "GIHJHMOALN_272", "locus": "GCA009939745_00236" }, { "type": "cds", "sequence": "contig_1", "start": 138053, "stop": 138439, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "TTGTTAAAGATCTTGGACCTCAACAAGATTAAAGAGGATGAAATTTTAAGCACCACTAAACTAGAGAAACACGAAAAACTCAATGCTTACGATGCGTTGCAAAAGCTACGCACTAAACTGCAAACCTTGGGCTTTCACAATGTGATTACTTACCAGTTAATTTCACCAGAACGAGCGCGTAACTTTAACCTGTTTGGGTTGAGTAATTTGTGGGAAATTAAAAACCCGCTTTCCAACGAACGGAGTGTTTTACGGGTTGGTTTAATTGACTCCTTGTTGCGAGTAATCCAAAAGAATGCCGCATACAAAAATAAGTTAGGCAACATCTTTGAGTTTTCTTTTGTCAAAACTAAGGACAGTAACCAACTCCACATTGCTGCTCTTTGA", "aa": "MLKILDLNKIKEDEILSTTKLEKHEKLNAYDALQKLRTKLQTLGFHNVITYQLISPERARNFNLFGLSNLWEIKNPLSNERSVLRVGLIDSLLRVIQKNAAYKNKLGNIFEFSFVKTKDSNQLHIAAL", "aa_hexdigest": "ca1e487d8471db22b18d8e51c488f62c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 111, "aa_cov": 0.8125, "hmm_cov": 1.0, "evalue": 6.593010382835894e-20, "score": 66.95671844482422, "start": 18, "stop": 121 } ], "seq_stats": { "molecular_weight": 14809.074999999983, "isoelectric_point": 9.733388328552245 }, "id": "GIHJHMOALN_273", "locus": "GCA009939745_00237" }, { "type": "cds", "sequence": "contig_1", "start": 138449, "stop": 139048, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "ATGTTTGGTTCTACCTACCAAAAGGATCAAGGCGTTAGTGTCGATATTCCCGCCATGAAGGGCTTAGCGCAGTTAATTATTTCCAACTTTGGTTTTAACTGCGACTTTGAACCAATTACTGAAGGTGAGTACTTCACTAAGAACGTCGGTTTAAAACTAGTGGTGTTTAACGAACAGATTGGTTATGTTGGTTTAATTAAGGATGAGCTGTTGGCGCCTTATGATTTAAAGGGTAGACCGGTGTATGGTTTGGAAATTAACCTAGACCGCTTGTTGAACTCCTTAAACCGGTTGGAGCGCAGTTATACCCCGATTAGCAAACTACAAGATGTCTTTAAGGATATTACCTTTAGCTTCCCCCGTGATGAAAGTCACTTTGAAACGTTTGTGAAAGCGATTAAAAAACTACAAACGATTTTTAAATGGGAATTGATCTCGGTGTTTGACACCGAAAAGGATGGTGTACCAATCACCAAATATACAGTGCGCTATTACTTAAAGAATTTCACTAATACACCATTAACGTTAGAACAAATTAAAGCAGTGGAAACGCAGTTAAAACAACAGTGTGAACTAGCTAAAATAGCACTCGATCTTTAG", "aa": "MFGSTYQKDQGVSVDIPAMKGLAQLIISNFGFNCDFEPITEGEYFTKNVGLKLVVFNEQIGYVGLIKDELLAPYDLKGRPVYGLEINLDRLLNSLNRLERSYTPISKLQDVFKDITFSFPRDESHFETFVKAIKKLQTIFKWELISVFDTEKDGVPITKYTVRYYLKNFTNTPLTLEQIKAVETQLKQQCELAKIALDL", "aa_hexdigest": "3080ec9fa8f0eeee8f5be8ddf20281b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 81, "aa_cov": 0.4020100502512563, "hmm_cov": 0.9753086419753086, "evalue": 3.54478709893245e-09, "score": 31.887662887573242, "start": 9, "stop": 88 } ], "seq_stats": { "molecular_weight": 22883.141000000014, "isoelectric_point": 5.5789403915405265 }, "id": "GIHJHMOALN_274", "locus": "GCA009939745_00238" }, { "type": "cds", "sequence": "contig_1", "start": 139161, "stop": 139916, "strand": "+", "frame": 3, "gene": "ytxK", "product": "putative protein MPN_107", "db_xrefs": [ "COG:COG0827", "COG:L", "RefSeq:WP_014325347.1", "SO:0001217", "UniParc:UPI0002386F70", "UniRef:UniRef100_A0AB33HQH8", "UniRef:UniRef50_P75562", "UniRef:UniRef90_P75562" ], "nt": "ATGACAAACCAAAACCTACAAGACTTTCTGGACGGAGTTTTGTTAAAAGAGGAAAATTTAAACAAAATTACTGAAGTTTCCCTAGAAAAGGGATTACCGCACCTTTTAGATGAGGGTATCCATAACGAAGATAACTTTCAGATCTTTACGAGCCAAAAGATTGTGGCAGACATGATCAAAATGGTCGGTGTAAAGGAAATGAAAAATGTCCACACTACTGTTTTAGAACCAACTAGTGGTGATGGTGCTTTTACTTGTGCTATTTTGGATTTACGTTTAAAAAACATTAAAAAATCAGACAGCTTTTTAAAAGAAGCTTTAACCGGTCTATCCACCATTTATTCAGTTGAAATTGACGAAGAGTTGGTTTGGTTACAACGCAATAACCTTTACTCTATCTTTTTAGCCTTCTTAAAAAAGCACGGTATTTTTAACGAAGAGTTAAGTCTCTTAGTGAAAAAAATCTTGATGATTAATGTGAGTTGGGGCGAGTTAATCACAGAAACCAAAGATAACGAGAACTTCGTTTGTAACTACGATTATTTTTTAAGGAATAAAAAGACAAAGAAAAAACCCGCAAAGCATAAACATGAATTTCCAGAGGGTTTATTTTCCAATGGAGAAGGTAAGTGCAAAAGTTTGTTTGTTAATTGGTCATTTGCTGATGTCATTAATGAAATTGCTCATAATTCCAATTTTGACTTTGCCAAAATAGCAAAGATACCTGGTGAAATAATTCAATTTCAAATTAATTAG", "aa": "MTNQNLQDFLDGVLLKEENLNKITEVSLEKGLPHLLDEGIHNEDNFQIFTSQKIVADMIKMVGVKEMKNVHTTVLEPTSGDGAFTCAILDLRLKNIKKSDSFLKEALTGLSTIYSVEIDEELVWLQRNNLYSIFLAFLKKHGIFNEELSLLVKKILMINVSWGELITETKDNENFVCNYDYFLRNKKTKKKPAKHKHEFPEGLFSNGEGKCKSLFVNWSFADVINEIAHNSNFDFAKIAKIPGEIIQFQIN", "aa_hexdigest": "fd361566dde6ea7cdb5baeface33f39f", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI0002386F70", "ncbi_nrp_id": "WP_014325347.1", "uniref100_id": "UniRef100_A0AB33HQH8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386F70", "RefSeq:WP_014325347.1", "UniRef:UniRef100_A0AB33HQH8" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HQH8", "uniref90_id": "UniRef90_P75562" }, "psc": { "uniref90_id": "UniRef90_P75562", "gene": "YtxK", "product": "Uncharacterized protein MPN_107", "uniref50_id": "UniRef50_P75562", "cog_id": "COG0827", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_P75562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75562" ], "product": "Uncharacterized protein MPN_107" }, "genes": [ "ytxK" ], "id": "GIHJHMOALN_275", "locus": "GCA009939745_00239" }, { "type": "cds", "sequence": "contig_1", "start": 139918, "stop": 140064, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTCAAAATGGCGATAAAGACCACGAATTGATTCAGGGAAAGGTCAAAAGCAAAACTGAAATAGAAGATTCCGTTATATTGGATCAAGACACTTTGCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGA", "aa": "MSQNGDKDHELIQGKVKSKTEIEDSVILDQDTLQGKGEEKTKLDELIR", "aa_hexdigest": "f3f5d9b52d27eb5d5157aa0bf9fa26b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5440.999399999999, "isoelectric_point": 4.749886894226075 }, "id": "GIHJHMOALN_276", "locus": "GCA009939745_00240" }, { "type": "cds", "sequence": "contig_1", "start": 140272, "stop": 140454, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAAGTTATACCGGATGAATATGAACGCAAGAAACACATCATGACCAAGATGCTTTTTAGTATTGACTTAACTAAATTTACTAGCTTGATGGTTCGTCGTACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAACTAGTGAAGATGAGGGCTGTGCAATTGCAAATGGCGCATGA", "aa": "MAKVIPDEYERKKHIMTKMLFSIDLTKFTSLMVRRTLYYCIKANKRKTSEDEGCAIANGA", "aa_hexdigest": "13a6ddf81ceaae4e007af63f4d0b1fd7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6960.175300000001, "isoelectric_point": 9.468423271179201 }, "id": "GIHJHMOALN_277", "locus": "GCA009939745_00241" }, { "type": "cds", "sequence": "contig_1", "start": 140423, "stop": 140614, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGGGCTGTGCAATTGCAAATGGCGCATGATTTAATAACGAATGCGGTAATGTTGTTAAACCTTACAAGGAACATTATTTCGACAAACAAGGAAAAAATAAAAAATGTAAGTTTTGTAGAACAGATGAGAAATTTAAATACCAACAATCTTCTTCAAATGAAAAATACGCCTATGACTTCATTCATTTAA", "aa": "MRAVQLQMAHDLITNAVMLLNLTRNIISTNKEKIKNVSFVEQMRNLNTNNLLQMKNTPMTSFI", "aa_hexdigest": "0fdad38dbf56bf509e89c74389e95626", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7290.559700000002, "isoelectric_point": 10.432676887512205 }, "id": "GIHJHMOALN_278", "locus": "GCA009939745_00242" }, { "type": "cds", "sequence": "contig_1", "start": 140659, "stop": 140823, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07669.17" ], "nt": "ATGAAATTTGATGTAATTATTGGTAATCCACCTTATCAGTTAGCAAATAATCGTGGTGGTGATGCTGGTGATGGTAATGGAGCAAACCCCATTTTTCAAGAATTTATCTTAAGAGCACTTGAATTACAACCAAAATATTTAGCAATGATTGTTCCTGCAAGATGA", "aa": "MKFDVIIGNPPYQLANNRGGDAGDGNGANPIFQEFILRALELQPKYLAMIVPAR", "aa_hexdigest": "0be93b2c684b66dce19192d4acd70eb0", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07669.17", "name": "Eco57I", "length": 65, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.7734658577762864e-14, "score": 49.69576644897461, "start": 1, "stop": 54 } ], "seq_stats": { "molecular_weight": 5915.756799999999, "isoelectric_point": 6.0008562088012685 }, "id": "GIHJHMOALN_279", "locus": "GCA009939745_00243" }, { "type": "cds", "sequence": "contig_1", "start": 141467, "stop": 141718, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2B7000", "UniRef:UniRef90_UPI00132F963A" ], "nt": "ATGCCCAGAATAACTGGTAGCGGTGTGTTTGGTGATTACAGACCCAATATAAAAATTGTTGAACCTACTGGAGTTTGCACTCATAGTTATGTAGTTGCTTATTTAACGACAAATAAGTTTGAAGTTGAAAATGTCTTTTTATACATGAAAACTAAATTTTTTAGATTTTTTGTCGAGATTTTTAAAGCAACCATCAATATCAGTTCACATAACTTCAAATACGTTCCCATTCAAGATTTTTCCCGTCCTTGA", "aa": "MPRITGSGVFGDYRPNIKIVEPTGVCTHSYVVAYLTTNKFEVENVFLYMKTKFFRFFVEIFKATINISSHNFKYVPIQDFSRP", "aa_hexdigest": "836e99bdcc47ec468f96fde1a0b7ec41", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 141467, "stop": 141805, "observations": { "mutation": [ 141716, 141764 ], "stop": [ 141716, 141764 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00132F963A", "query_cov": 1.0, "subject_cov": 0.7410714285714286, "identity": 0.988, "score": 168.0, "evalue": 1.29e-52, "gene_start": 1, "gene_end": 83, "reference_start": 1, "reference_end": 83, "reference_sequence": "MPRITGSGVFGDYIPNIKIVEPTGVCTHSYVVAYLTTNKFEVENVFLYMKTKFFRFFVEIFKATINISSHNFKYVPIQDFSRPWNDRELYQKYNLTQEEWQYIENNISSYEN" }, "paralog": false, "identity": 0.9642857142857143, "score": 221.0, "evalue": 6.29e-76, "description": "Internal stop codon at 141716, 141764. Nonsense mutation around 141716, 141764.", "psc": { "uniref90_id": "UniRef90_UPI00132F963A", "uniref50_id": "UniRef50_UPI001F2B7000", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00132F963A", "UniRef:UniRef50_UPI001F2B7000" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2B7000", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2B7000" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9694.13059999999, "isoelectric_point": 9.415430259704593 }, "id": "GIHJHMOALN_280", "locus": "GCA009939745_00244" }, { "type": "cds", "sequence": "contig_1", "start": 142363, "stop": 142524, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGAAAGATACTTTCAAGCCAATCCCGAAAACGGTAGGTTTTTAATGAACTGCAAAATGCGGTTCGGTAAATGTTTCACCTTGTACTCTTATGCACAAAAGAATAACATTAACAAGATTTTGATCCTAACGTTTGTTCCAGCTGTCGAAGAATCTTGA", "aa": "MLERYFQANPENGRFLMNCKMRFGKCFTLYSYAQKNNINKILILTFVPAVEES", "aa_hexdigest": "874a998ad47ce4006dee46d92458f397", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6283.305899999999, "isoelectric_point": 9.138022804260256 }, "id": "GIHJHMOALN_281", "locus": "GCA009939745_00245" }, { "type": "cds", "sequence": "contig_1", "start": 142579, "stop": 143433, "strand": "+", "frame": 1, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HLI1", "UniRef:UniRef90_UPI0036F40F46" ], "nt": "TTGCAAAAATCTAATTTTGATTTAAAGAATCAAAACGAGCCTTATGTAGTCTTTTTATCACTGCAAAACGTCTTAGGTAAACAAAGAATAGATGGCGCCAAAACTGATTTTGATAAAGAGCGAATTAGTAAGCTACAAGAGATTGATTTTGATTTAATTGTTTTTGATGAGTACCACTACGGAGCCAACAAAAAACGAACCCAAATCAAAGTTGAAAAAGTCAATAAAAAGATTGATAATCCAGAACAGCAAGATAATCAAGATGTTGCTGAGGAAGAATTGGCAAGTACTTTTAAGTTAAAAGACATCAAAAGTAAATTTCAATTTTCATACAAACAATTAGTTTGTCTGTCAGGTACTCCTTTTTCCTCTTTAAGAAATAATGAGTTTTCCAGTAAAGATCAGGTGTTTACTTATTCTTACTTTGATGAACAGAAGGCCAAATCAGCAGAAAATCACCCACTCAAATTAGGGCAATACGGAATATTTCCGGAAATGAATATTTACTGTTTTGAATTGGCGGAAATTTTTACTGCACAAGAACAAGAAATATTCATAACTCCAGGTAAAGGTAAAAATAAGTTACCTGAAATTAGTTTTCGAAAGCTGTTTCAAACGGAAAACGTTTCTAAAGAATCAAGCAAACCAGTTTACAGATTTGTAAATGAAAACCTGGTTGAAAAGCTTATAGATTCTTTAATTGATAAACGTAAAGGTTTTAGCCACACACCTTTAAGTTGGGAAAACATTGACAAGCATAAGCATTCCTTATTAATTTTGCCAACTAGAGTTGCTTGTTTTGCGTTGGCCAATTTACTTAAAAATCACTGGTACTTTGAAAACAATGATTTTTAA", "aa": "MQKSNFDLKNQNEPYVVFLSLQNVLGKQRIDGAKTDFDKERISKLQEIDFDLIVFDEYHYGANKKRTQIKVEKVNKKIDNPEQQDNQDVAEEELASTFKLKDIKSKFQFSYKQLVCLSGTPFSSLRNNEFSSKDQVFTYSYFDEQKAKSAENHPLKLGQYGIFPEMNIYCFELAEIFTAQEQEIFITPGKGKNKLPEISFRKLFQTENVSKESSKPVYRFVNENLVEKLIDSLIDKRKGFSHTPLSWENIDKHKHSLLILPTRVACFALANLLKNHWYFENNDF", "aa_hexdigest": "babdcd386b70a0f192cecaf4bbe24ac6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 142280, "stop": 143529, "observations": { "deletion": [ 142313 ], "stop": [ 142523, 143432 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0036F40F46", "query_cov": 1.0, "subject_cov": 0.6604651162790698, "identity": 0.993, "score": 562.0, "evalue": 4.36e-200, "gene_start": 1, "gene_end": 284, "reference_start": 113, "reference_end": 396, "reference_sequence": "MKIRISLNILAKFLDSFAHLLFLKNKEIYTPRKEQAACVEVLERYFQANPENGRFLMNCKMRFGKCFTLYSYAQKNNINKILILTFVPAVEESLKDDLNHIEKNYKFYTDDDLQKSNFDLKNQNEPYVVFLSLQNVLGKQRIDGAKTDFDKERISKLQEIDFDLIVFDEYHYGANKKRTQIKVEKVNKKIDNPEQQDNQDDAEEELASTFKLKDIKSKFQFSYKQLVCLSGTPFSSLRNNEFSSKDQVFTYSYFDEQKAKSAENHPLKLGQYGIFPEMNIYCFELAEIFTAQEQEIFITPGKGKNKLPEISFRKLFQTENVSKESSKPVYRFVNENLVEKLIDSLIDKRKGFSHTPLSWENIDKHKHSLLILPTRVACFALANLLKNHWYFENNDFQIINMSESQFGNGKKALIELNKHLDEAKKTNKIL" }, "paralog": false, "identity": 0.9903846153846154, "score": 826.0, "evalue": 1.58e-306, "description": "Internal stop codon at 142523, 143432. Frameshift due to deletion around 142313.", "psc": { "uniref90_id": "UniRef90_UPI0036F40F46", "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_A0AB33HLI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0036F40F46", "UniRef:UniRef50_A0AB33HLI1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HLI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HLI1" ] } }, "id": "GIHJHMOALN_282", "locus": "GCA009939745_00246" }, { "type": "cds", "sequence": "contig_1", "start": 143443, "stop": 143583, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAGAGCCAGTTTGGTAATGGCAAAAAAGCTTTAATTGAGCTTAATAAACACTTAGATGAAGCTAAAAAGACTAATAAAAATACTTTAACTATTACTGTAGCTAAATTAACTATTGGTATTACAGTTAAAGAGTGA", "aa": "MSESQFGNGKKALIELNKHLDEAKKTNKNTLTITVAKLTIGITVKE", "aa_hexdigest": "35032b3c70f7297e321738f585ca3582", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5056.831800000001, "isoelectric_point": 9.523866081237792 }, "id": "GIHJHMOALN_283", "locus": "GCA009939745_00247" }, { "type": "cds", "sequence": "contig_1", "start": 143599, "stop": 143787, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGGATTTAAAGGGTGCTGAATCTTATTTCCAAACGATTTTTAGAATCCAAACTCCATATATAAAGAACTGTAAAAATCTGAAAGAAATTTGTTATGTTTACGACTTCAACATGTATAGGTGTTTGGAAGTTACTAACGAGTATTCAAAACAAACACAAACAGACCCTAAATTTAGTGCTAGTTGA", "aa": "MKDLKGAESYFQTIFRIQTPYIKNCKNLKEICYVYDFNMYRCLEVTNEYSKQTQTDPKFSAS", "aa_hexdigest": "793492b363d0808a3826746ea9fc44b2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7435.424700000002, "isoelectric_point": 8.458783912658692 }, "id": "GIHJHMOALN_284", "locus": "GCA009939745_00248" }, { "type": "cds", "sequence": "contig_1", "start": 143917, "stop": 144162, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAAAGTAACATTATCGACATAGATGTACTTTGCAATGTTGGGCAAGATGAAGATGCGCAAAAAATATTGAAAAAAATATTAGCTCATAAAAAATTTAAATCATCCAAAAAGAAGCGAGAGTTTGAAGACGTTCATTTGGAAAAAAGTCCAAAAAGTGAGGCATTTAGTGAAGGCGTTCGCTCTGGTAAGGATTACGCCGCTGAACAAGGTAATGTTTTAGAAGAACTTGAAAATTTCTGA", "aa": "MDESNIIDIDVLCNVGQDEDAQKILKKILAHKKFKSSKKKREFEDVHLEKSPKSEAFSEGVRSGKDYAAEQGNVLEELENF", "aa_hexdigest": "ffba8b18e2dc67ab777a0ede53ab4c82", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9226.243599999996, "isoelectric_point": 5.168903923034666 }, "id": "GIHJHMOALN_285", "locus": "GCA009939745_00249" }, { "type": "cds", "sequence": "contig_1", "start": 144305, "stop": 144472, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCAGAATAAAATCAAAGACTTTAAAAAAAGAAAAGGAAGAACAACTTCAACTTTTAAAAAATGAAAATGGTTATATTTCTGATGAGAATAACTTAAAAAAGCTAAGAGATTTAATTAAAGAATATGTACAAAACAGAAGCAACATACCTCCAGAATTAGACTGA", "aa": "MFRIKSKTLKKEKEEQLQLLKNENGYISDENNLKKLRDLIKEYVQNRSNIPPELD", "aa_hexdigest": "1eecdc65706310bc9296577b921531cf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6633.5424, "isoelectric_point": 9.003348350524902 }, "id": "GIHJHMOALN_286", "locus": "GCA009939745_00250" }, { "type": "cds", "sequence": "contig_1", "start": 144599, "stop": 144853, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU34", "UniRef:UniRef90_A0AB33HU34" ], "nt": "ATGCATACATGGGCACGTGTTCTGCACTTTCTTTATGTATCTGTTTATAGAGAAAAAACTTTAAGAGATGTGTTGAATAAAACTGATCCCAAAGTTTTTTCAGTGGCAGTCGGAATTGAAATGGATGAGTTTTCCAAAATCCAAAAATTTTTTAAAGTGGAAAATATGAATAGACTCATCGAACAGTTTAGTTTTAGAGAAAGTGTTAAAACTGAAAACACAGAAAAATCACAGTATAGAAACAGTTTCAATTGA", "aa": "MHTWARVLHFLYVSVYREKTLRDVLNKTDPKVFSVAVGIEMDEFSKIQKFFKVENMNRLIEQFSFRESVKTENTEKSQYRNSFN", "aa_hexdigest": "e12e6933ae29fb8dd708477d305bc870", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 144305, "stop": 144886, "observations": { "mutation": [ 144470, 144851 ], "stop": [ 144470, 144851 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HU34", "query_cov": 1.0, "subject_cov": 0.43523316062176165, "identity": 1.0, "score": 166.0, "evalue": 9.79e-51, "gene_start": 1, "gene_end": 84, "reference_start": 99, "reference_end": 182, "reference_sequence": "LFRIISKTLKKEKEEQLQLLKNENGYISDENNLKKLRDLIKEYVQNRSNIPPELDWFFYRDRFIQLFEKSCYQHFKKKTLTEEQKEKKKKVNDNFDSLMHTWARVLHFLYVSVYREKTLRDVLNKTDPKVFSVAVGIEMDEFSKIQKFFKVENMNRLIEQFSFRESVKTENTEKSQYRNSFNWFGFGPDSLKS" }, "paralog": false, "identity": 0.9844559585492227, "score": 379.0, "evalue": 6.79e-137, "description": "Internal stop codon at 144470, 144851. Nonsense mutation around 144470, 144851.", "psc": { "uniref90_id": "UniRef90_A0AB33HU34", "uniref50_id": "UniRef50_A0AB33HU34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HU34", "UniRef:UniRef50_A0AB33HU34" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HU34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU34" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10157.494499999995, "isoelectric_point": 9.344708442687988 }, "id": "GIHJHMOALN_287", "locus": "GCA009939745_00251" }, { "type": "cds", "sequence": "contig_1", "start": 144903, "stop": 145049, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAATAGTGGTAAAGACTACGAATCGATTCAAGGTAAGATTAAGAGTAAAGCTGAAATAGAAGATTCAGTTATATTGGAAAAAGAAACTTTGCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGA", "aa": "MNKNSGKDYESIQGKIKSKAEIEDSVILEKETLQGKGEEKTKLDELIR", "aa_hexdigest": "49cd77569ed271ca55b0f3753a38abbd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5452.108199999999, "isoelectric_point": 5.364145851135253 }, "id": "GIHJHMOALN_288", "locus": "GCA009939745_00252" }, { "type": "cds", "sequence": "contig_1", "start": 145302, "stop": 145799, "strand": "+", "frame": 3, "gene": null, "product": "Eco57I restriction-modification methylase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132FCD8E", "UniRef:UniRef90_UPI00133023F0" ], "nt": "ATGACCAAGATGCTTTTTAGCATTGGCTTAACTAAATTTACTAGTTTGATGGTGCGTCGCACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAATCAGCGAAGATGAGGGTTGTGCAATTGCAAATGGCGCACGATTTAATAATGAATTTGGCAATGTTGTTACCCCTTATAAAGAACACTATTTTGGAAAAGAATCGAAAAGCAAAAACTGTCACTTTTGTGGGACAAATAAGAATTCTAAATATGTAAATTCTATGACTGAAGAAAAGTATGCCTATGACTTCATTCATTTAAATTCGGATGAATATGAAAACTACTTTAAAACTAATTTTGGTGTTATGAAATTTGATGTAATTATTGGAAATCCGCCTTACCAGCTATCTAATGGTAGCGGTTCTGATGGCAATGGAGCCAAAGCAATTTTTCAAGATTTTGTTTTAAAAGCAATTGACTTAGAACCAAAATATTTAGCGATGATTATCCCAGCAAAATGA", "aa": "MTKMLFSIGLTKFTSLMVRRTLYYCIKANKRKISEDEGCAIANGARFNNEFGNVVTPYKEHYFGKESKSKNCHFCGTNKNSKYVNSMTEEKYAYDFIHLNSDEYENYFKTNFGVMKFDVIIGNPPYQLSNGSGSDGNGAKAIFQDFVLKAIDLEPKYLAMIIPAK", "aa_hexdigest": "c9304ab361307b4525c7e6e2730f9ee2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 145002, "stop": 145837, "observations": { "deletion": [ 145815 ], "mutation": [ 145047, 145170, 145191, 145797 ], "stop": [ 145047, 145170, 145191, 145797 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00133023F0", "query_cov": 1.0, "subject_cov": 0.532258064516129, "identity": 0.9940000000000001, "score": 334.0, "evalue": 4.45e-114, "gene_start": 1, "gene_end": 165, "reference_start": 134, "reference_end": 298, "reference_sequence": "MNKNSGKDYESIQGKIKSKAEIEDSVILEKETFQGKGEEKTKLDELIRWEAEKNDLLKLIDNVGNDEVFTPVETCQRMLDELFPEDHEVWSNPDLKWLNPCDKNGVFFREIALRLDKGLTKIIPDEYARKKHIMTKMLFSIGLTKFTSLMVRRTLYYCIKANKRKTSEDEGCAIANGARFNNEFGNVVTPYKEHYFGKESKSKNCHFCGTNKNSKYVNSMTEEKYAYDFIHLNSDEYENYFKTNFGVMKFDVIIGNPPYQLSNGSGSDGNGAKAIFQDFVLKAIDLEPKYLAMIIPAKWMISAKYFFKSK" }, "paralog": false, "identity": 0.9784172661870504, "score": 542.0, "evalue": 2.98e-198, "description": "Internal stop codon at 145047, 145170, 145191, 145797. Frameshift due to deletion around 145815. Nonsense mutation around 145047, 145170, 145191, 145797.", "psc": { "uniref90_id": "UniRef90_UPI00133023F0", "product": "Eco57I restriction-modification methylase domain-containing protein", "uniref50_id": "UniRef50_UPI00132FCD8E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00133023F0", "UniRef:UniRef50_UPI00132FCD8E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00132FCD8E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132FCD8E" ], "product": "Eco57I restriction-modification methylase domain-containing protein" } }, "id": "GIHJHMOALN_289", "locus": "GCA009939745_00253" }, { "type": "cds", "sequence": "contig_1", "start": 145800, "stop": 146087, "strand": "+", "frame": 3, "gene": null, "product": "Eco57I restriction-modification methylase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F3844D4", "UniRef:UniRef90_UPI001F3844D4" ], "nt": "ATGATTTCTGCTGAAAATATTTTTTTAAATCTAAGAGATAAATTAAAGAAAAATAAAGGTATAAAAGAAATTAATATTTTTTTCGACTCTAAAGACTGTTTTCCAAAGAGAGAAATAAAAGGTGGCATTTGTTACTTTATCTGGCAGAATAACTATCAAGGTAAGACTCTTATAAATACCAAATTTTCAAAGAAAGGAAAAAGCACAGAGATCGATAGTTCTAAAAGAAACTTATTTGTTTCCGTTTTAAACAACACTGAACAAATCATTTTTCGCAAAAGGATTTGA", "aa": "MISAENIFLNLRDKLKKNKGIKEINIFFDSKDCFPKREIKGGICYFIWQNNYQGKTLINTKFSKKGKSTEIDSSKRNLFVSVLNNTEQIIFRKRI", "aa_hexdigest": "55bb0d021f9cc803407b3e29c114b6ed", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 145815, "stop": 146171, "observations": { "mutation": [ 146085 ], "stop": [ 146085 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F3844D4", "query_cov": 0.9473684210526315, "subject_cov": 0.7627118644067796, "identity": 0.978, "score": 179.0, "evalue": 1.38e-56, "gene_start": 6, "gene_end": 95, "reference_start": 1, "reference_end": 90, "reference_sequence": "NNFLNLRDKLKKNKDIKEINIFFDSKDCFPKREIKGGICYFIWQNNYQGKTLINTKFSKKGKSTEIDSSKRNLFVSVLNNTEQIIFRKRIWQDIYTKIDSISASEMKNTHTEREQFRS" }, "paralog": false, "identity": 0.9745762711864406, "score": 231.0, "evalue": 1.31e-79, "description": "Internal stop codon at 146085. Nonsense mutation around 146085.", "psc": { "uniref90_id": "UniRef90_UPI001F3844D4", "product": "Eco57I restriction-modification methylase domain-containing protein", "uniref50_id": "UniRef50_UPI001F3844D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F3844D4", "UniRef:UniRef50_UPI001F3844D4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F3844D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F3844D4" ], "product": "Eco57I restriction-modification methylase domain-containing protein" } }, "id": "GIHJHMOALN_290", "locus": "GCA009939745_00254" }, { "type": "cds", "sequence": "contig_1", "start": 146254, "stop": 146724, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_026088310.1", "SO:0001217", "UniParc:UPI0004755848", "UniRef:UniRef100_A0AB38WA20", "UniRef:UniRef50_A0AB38WA20", "UniRef:UniRef90_A0AB38WA20" ], "nt": "TTGAAAGCAGAAAAATTTCCCGGTTTGATTGCAATTTATGGAAGTCATTCGGTTATTAAACACGTGGATATTAAAGATATTCCGCAAATAAAAAATAATGAAGAAATTGCTAAATACAAAATTATTGTTCCACACGTTTATAGCCGTGGCAATGGAACTTTTGGAAATTACAAACCAAAAGTAAAAATTATTAAACCTAATGAAATTTGCACCGAAACTTATTTAGTGGTATATCCCACCGAAAGTAAAGTTGAGATTGAAAACGTCGCTTCTTATATGAGAACTAAATTTTTCAGATTTTTAGTTGAAATTTTTAAGGATTCTATTAACACAAATTCTCAAAATTTCAAGTTTATTCCTCTACAAGATTTTTCACGTCCTTGGAACGATAGAGAACTTTATGAAAAATACGGTTTAACACTCGAAGAACAGCAATACATCGAGGCGAACATAAGTGCTTACGAGGACTAA", "aa": "MKAEKFPGLIAIYGSHSVIKHVDIKDIPQIKNNEEIAKYKIIVPHVYSRGNGTFGNYKPKVKIIKPNEICTETYLVVYPTESKVEIENVASYMRTKFFRFLVEIFKDSINTNSQNFKFIPLQDFSRPWNDRELYEKYGLTLEEQQYIEANISAYED", "aa_hexdigest": "c0716801b80d9d4d8bec1f38971cef67", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0004755848", "ncbi_nrp_id": "WP_026088310.1", "uniref100_id": "UniRef100_A0AB38WA20", "db_xrefs": [ "SO:0001217", "UniParc:UPI0004755848", "RefSeq:WP_026088310.1", "UniRef:UniRef100_A0AB38WA20" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38WA20", "uniref90_id": "UniRef90_A0AB38WA20" }, "psc": { "uniref90_id": "UniRef90_A0AB38WA20", "uniref50_id": "UniRef50_A0AB38WA20" }, "pscc": { "uniref50_id": "UniRef50_A0AB38WA20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38WA20" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18312.711499999994, "isoelectric_point": 6.843323707580566 }, "id": "GIHJHMOALN_291", "locus": "GCA009939745_00255" }, { "type": "cds", "sequence": "contig_1", "start": 147031, "stop": 147423, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_112", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874469.1", "SO:0001217", "UniParc:UPI000013A1CB", "UniRef:UniRef100_P75450", "UniRef:UniRef50_P75450", "UniRef:UniRef90_P75450" ], "nt": "ATGCTAGATAAATTACTCCAGAAATTTCGAGACCAAAAGAAGCCCGTCTTCCACAAAGAAGAGGGCTACTGGGAAATTAGTGCCTTGCGGAAATGGGCAGCCATTTTAATAATTGCCTTTGGAGCTGGCATTATTTACATTGTGCCCTACTTTGCCTTCTTTCAGTTTAAGACAGCAGTAGCTAATGTAACGGGAGTGGAGCCCAACAGAATCTCCCTTCTCTTAACTGCTTACGGGATTGTTTCATTGCTGTTTTATATTCCTGGTGGTTGGTTAGCTGACCGGATTTCAGCTAAAGCGCTGTTTTCGGTATCAATGTTTGGTACTGGCATTATTACCTTCTGGTACTTCTTAGTTGGTTTGAAGGGCATAGTGTGGATTACACCCAACTAG", "aa": "MLDKLLQKFRDQKKPVFHKEEGYWEISALRKWAAILIIAFGAGIIYIVPYFAFFQFKTAVANVTGVEPNRISLLLTAYGIVSLLFYIPGGWLADRISAKALFSVSMFGTGIITFWYFLVGLKGIVWITPN", "aa_hexdigest": "09afb655a9cf37dc9cec4897c8f01479", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A1CB", "ncbi_nrp_id": "WP_010874469.1", "uniref100_id": "UniRef100_P75450", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A1CB", "RefSeq:WP_010874469.1", "UniRef:UniRef100_P75450" ] }, "ips": { "uniref100_id": "UniRef100_P75450", "uniref90_id": "UniRef90_P75450" }, "psc": { "uniref90_id": "UniRef90_P75450", "product": "Uncharacterized protein MPN_112", "uniref50_id": "UniRef50_P75450", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75450", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75450" ], "product": "Uncharacterized protein MPN_112" }, "genes": [], "id": "GIHJHMOALN_292", "locus": "GCA009939745_00256" }, { "type": "cds", "sequence": "contig_1", "start": 147405, "stop": 147893, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTACACCCAACTAGTTATTATCCACGTCCTGTATGCCTTTAGTACGGTCTTAACCTTCTGGAGTGCCTTCATTAAAAGTATTAAGTTATTGGGTCCAGCGGATGAACAAGCGAGGTTGTATTCCACATCTGAAGGATCACGCTACTTAATCCAAGTAATTACTGGTTACATTAGTGCTGGTTTGGGTGCTATTGTCCTTATTAGTACTCCCTTTCTCATTTACAAAGCACCAACTGCTGCTGACCCTAAAAACTTAGCTTCTTCCTCAGGGATCTTCTTTGCCTTACTGTTTTATGCCATTTGTTATGTGTTAGCTGGTTTACTGTCACTGTTTGTTTTACCAGGAAAGTTCTGGGAAAAGCGCAAGATTTACTTGGAAGATGAACGAATTGAATACTTTAACCCTGTGGACCAGGCTTATCACATCTTTAAATCGGAAGCAGAATTAGAAGCCTTTAAGAAACATAACACTAAGGTTTTTTGA", "aa": "MDYTQLVIIHVLYAFSTVLTFWSAFIKSIKLLGPADEQARLYSTSEGSRYLIQVITGYISAGLGAIVLISTPFLIYKAPTAADPKNLASSSGIFFALLFYAICYVLAGLLSLFVLPGKFWEKRKIYLEDERIEYFNPVDQAYHIFKSEAELEAFKKHNTKVF", "aa_hexdigest": "47bbaebd9abd6fbff6716384a52a23bc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18341.16129999999, "isoelectric_point": 7.786636543273926 }, "id": "GIHJHMOALN_293", "locus": "GCA009939745_00257" }, { "type": "cds", "sequence": "contig_1", "start": 147972, "stop": 148256, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGTGTACCAAACGATTGCTAACTTTGGTACTAACATTTTGGAATCGCTAGGACTAGAGGCAGCACTCTCCCCAACTTTAAGTAATACCTATATCTATGGGATTCCGGTGTTAGGGGCAATCTTTTCTGGTTTGATTAACAAAAAGTTAACTAAATCAACCGCTAAAAGTCTGATTGTGCAAGCCTTGTTTGTCATTTTGGGTACCTTAGCCTTTTTAGTAATAACACTAGCTTCCCCAGCCAAACCAGAAACTGGTGCATTTAACCAAGCTACCTTGTGA", "aa": "MNVYQTIANFGTNILESLGLEAALSPTLSNTYIYGIPVLGAIFSGLINKKLTKSTAKSLIVQALFVILGTLAFLVITLASPAKPETGAFNQATL", "aa_hexdigest": "c62f5efa1006dbd723ea99eb0a027294", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9873.500899999988, "isoelectric_point": 9.158330345153807 }, "id": "GIHJHMOALN_294", "locus": "GCA009939745_00258" }, { "type": "cds", "sequence": "contig_1", "start": 148284, "stop": 148502, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTTATTGGCGCGAACCGTTCCATTTTTTGGAGTACGATCACAGAACTGAAAGTCAATAAGGAAATAGTAGGACTGGCAGTTGGTTTTATTTCGATTATTGGTTTTTCCAAAGATGTGTGGTTAAGTCCCTTACTGACTGGTACTACTAACCAGTTCATTGTCAAAAACAGTCAGGGTACCTCGTTTTACAGCCAGCAAGCCTTGGTAGCCTGA", "aa": "MFFIGANRSIFWSTITELKVNKEIVGLAVGFISIIGFSKDVWLSPLLTGTTNQFIVKNSQGTSFYSQQALVA", "aa_hexdigest": "7ad1448c6ae1df7dfa260762d181f4f9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7932.1071999999995, "isoelectric_point": 9.398732948303223 }, "id": "GIHJHMOALN_295", "locus": "GCA009939745_00259" }, { "type": "cds", "sequence": "contig_1", "start": 148633, "stop": 148782, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACACTAAATTAACACCACCCTGGTTGCGTCAAGAAAATAACCTGCTCAACCCCGAAGTTACCCAACGTTTGTTTATCAACCAGGACAACATTCCGAACTTACCCACTGAAGCCCCCCAAGTTTATTTAGCCCGTTTTTTGGAATGA", "aa": "MDTKLTPPWLRQENNLLNPEVTQRLFINQDNIPNLPTEAPQVYLARFLE", "aa_hexdigest": "d35db3a995426340778277bb19f514a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5792.5352, "isoelectric_point": 4.800644111633301 }, "id": "GIHJHMOALN_296", "locus": "GCA009939745_00260" }, { "type": "cds", "sequence": "contig_1", "start": 148813, "stop": 149016, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGCACAAGCAGCAGTTCAGGATTACCTCAATTCTAAAGCCTGTGCACAAATTGAAACCATTATTGCAGAACGGGCGCAAAACACCCATTCGAGTTGGTTAGCTAACTGGTGGGTGCAGTACGCTTATTTAACTTCGACTGGCCCGGTTAGTCCGGAAGTTAATGCACCCTATTATTTAGAGCTCCCGACTGTTGGTTGA", "aa": "MKAQAAVQDYLNSKACAQIETIIAERAQNTHSSWLANWWVQYAYLTSTGPVSPEVNAPYYLELPTVG", "aa_hexdigest": "b09559ff02a9e4e227171cbef723aa7f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7460.283500000001, "isoelectric_point": 4.957690238952636 }, "id": "GIHJHMOALN_297", "locus": "GCA009939745_00261" }, { "type": "cds", "sequence": "contig_1", "start": 149134, "stop": 149409, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATCAATCTTTGCTAGTTGCCAGATTCACCGCGCTGATGGGGATGTGTACTTTGTTAATGATCAACCTGCTAACTTCATTGTGGTGATTAAGAACAATGTCTTCTATAAGTTAGTAATAGATAACAGTGGTAGCTTAGAACAACTGCAAGCGCAACTGCAGTTAAGCTTTGTGCAAATCTTAGACAATGAGCTAAGTCACCCACCGCACTGGAACTTACTCACTGCTACCACAACCAAAGCGGAGTCACAAAGTTTACTCGATCAGTTGTGA", "aa": "MQSIFASCQIHRADGDVYFVNDQPANFIVVIKNNVFYKLVIDNSGSLEQLQAQLQLSFVQILDNELSHPPHWNLLTATTTKAESQSLLDQL", "aa_hexdigest": "e3f5d7731d015054c906275db0fc817e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10274.47999999999, "isoelectric_point": 4.721694755554199 }, "id": "GIHJHMOALN_298", "locus": "GCA009939745_00262" }, { "type": "cds", "sequence": "contig_1", "start": 149425, "stop": 149517, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTGGACATCTATAACAGTGCCTTTATTGTGAACTTGGACAACGTCGAATTAACGACTCCCTTACAACTGTTGCGCAATTCGACATGA", "aa": "MLLDIYNSAFIVNLDNVELTTPLQLLRNST", "aa_hexdigest": "c17b110897154ee35c51f32e14948af8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3421.9122000000007, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_299", "locus": "GCA009939745_00263" }, { "type": "cds", "sequence": "contig_1", "start": 149557, "stop": 150207, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCATTACTAAGAACGCTCAGTTAGTCATTCTAGCGGATCACACTAGTTTTGACGGTTCTAGTGTAGCTACCCTAGCCAACATCTTTGTCTCTAAACTCCAAAAGGTCAACACTGAGGGTGCCAGCGCTTTAACACCGACCATGCTTTCCTTTCCAACAGTAGACCAGGATAAGCAAAAGTATTTTAAGCAATTGAGCAAAAATTTTAAGGACTATGTGTACAATGCCGTTATGTTTGAACTGAAATGGGACTGGTTTACGAAGCCACTTATCAAAGCCAAAGGGATTAAGAACTCCGAAGCCTTTATCCATCTCTGTTACCAAATTGCCCAGTACCAAACGAACAAAAAGTTGCAGAACACCTATGTGGCCGTGGACATGCGTCAGTACTTCCGGGGACGCACCGAGTGTCTTCGTCCTTTAAGTAAACAATCAGTTGCCTTTGTCAAACGCTACTGCAAAGATCCGAAAGGTACCTTAAAGCAGTTCCGAAAGTACTACCCAGCGATTGAAAGTCTCCACTTTGAAAAAACCCGGTTAGCGCAAAAGGGTAGTGGGGTCAACCGTCACTTGCTGGGAGCATATTTGGCTTGGAATGAACACCAAGACACAATAGCTAAACCCGCTTTATTTGAAACAAAGGCTTGA", "aa": "MVITKNAQLVILADHTSFDGSSVATLANIFVSKLQKVNTEGASALTPTMLSFPTVDQDKQKYFKQLSKNFKDYVYNAVMFELKWDWFTKPLIKAKGIKNSEAFIHLCYQIAQYQTNKKLQNTYVAVDMRQYFRGRTECLRPLSKQSVAFVKRYCKDPKGTLKQFRKYYPAIESLHFEKTRLAQKGSGVNRHLLGAYLAWNEHQDTIAKPALFETKA", "aa_hexdigest": "d7166ce915cb106519eeea40626def43", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24880.50170000001, "isoelectric_point": 9.722557640075681 }, "id": "GIHJHMOALN_300", "locus": "GCA009939745_00264" }, { "type": "cds", "sequence": "contig_1", "start": 150247, "stop": 150435, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGACAAGTATTTGCGCAACTTTTCCTTCGACCCCGTAGAGCCAAACGGCATTGGTATTGCCTATGCCATTGATGACACTAATTTCCGTGCCATTCTCAGTGTGTATCAGCACAACTTACAGTACCTCAAAGATTGGATGAAACACTTTGAACAAACAGTGAAAACAATCCTTAAAACTCTTAAATAA", "aa": "MDKYLRNFSFDPVEPNGIGIAYAIDDTNFRAILSVYQHNLQYLKDWMKHFEQTVKTILKTLK", "aa_hexdigest": "84e55af061b4a9a5d65ce1d90d3f824d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7380.4155, "isoelectric_point": 8.025427436828615 }, "id": "GIHJHMOALN_301", "locus": "GCA009939745_00265" }, { "type": "cds", "sequence": "contig_1", "start": 150525, "stop": 150941, "strand": "+", "frame": 3, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022", "SO:0001217", "UniRef:UniRef50_P47438", "UniRef:UniRef90_P78024" ], "nt": "ATGCCGTTGATTCTTGATCAGGGCTGTTTTGTTAGTGAAAGTAGATTTCATAGATTGGCACAGAACATAAAACAAGGTTCCAGGCGTGAGCGCGAACAAAAACCGTTAATTAACGATAAAATTGGCTTTAATGAGTTCATTTTAATTGACGAAAATGGCTCCAATTTGGGGACGGTTAGAAGGACCGATGCCCTCAAGATGGCTGAGGAAAAACAACTCGATTTAGTCTTAATTGGTTCAAACCCAGCCAAACCCATTGTTAAGCTCTTAGACTTTGGTCGTTACACTTATGATTTAAAACGCAAAAAACGCCAGTCCAAGAAAAACCAAACCATTATCCAAATAAAGGAAGTGGTAGTGAAACCGACAATCGCTAAACATGATCTGGAATTTAAAGCTAAGCAAACGACGGGATGA", "aa": "MPLILDQGCFVSESRFHRLAQNIKQGSRREREQKPLINDKIGFNEFILIDENGSNLGTVRRTDALKMAEEKQLDLVLIGSNPAKPIVKLLDFGRYTYDLKRKKRQSKKNQTIIQIKEVVVKPTIAKHDLEFKAKQTTG", "aa_hexdigest": "9f687e2474f49e8dd47239253ae7c8a5", "start_type": "ATG", "rbs_motif": null, "genes": [ "infC" ], "truncated": "3-prime", "pseudogene": { "start": 150525, "stop": 151130, "observations": { "mutation": [ 150939 ], "stop": [ 150939 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78024", "query_cov": 1.0, "subject_cov": 0.6865671641791045, "identity": 1.0, "score": 267.0, "evalue": 1.59e-89, "gene_start": 1, "gene_end": 138, "reference_start": 1, "reference_end": 138, "reference_sequence": "MPLILDQGCFVSESRFHRLAQNIKQGSRREREQKPLINDKIGFNEFILIDENGSNLGTVRRTDALKMAEEKQLDLVLIGSNPAKPIVKLLDFGRYTYDLKRKKRQSKKNQTIIQIKEVVVKPTIAKHDLEFKAKQTTGWAEKGYHVKFVVRAFGRVSTRIELIEKVFNDFYLLVEPAVEVQKPLTASSKTMYSALLVPRKK" }, "paralog": false, "identity": 0.9900497512437811, "score": 389.0, "evalue": 3.84e-140, "description": "Internal stop codon at 150939. Nonsense mutation around 150939.", "psc": { "uniref90_id": "UniRef90_P78024", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_P47438", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78024", "UniRef:UniRef50_P47438", "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P47438", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47438" ], "product": "Translation initiation factor IF-3" } }, "id": "GIHJHMOALN_302", "locus": "GCA009939745_00266" }, { "type": "cds", "sequence": "contig_1", "start": 150972, "stop": 151130, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGCGCTTTTGGTAGAGTAAGTACTAGGATTGAGTTAATTGAAAAAGTCTTTAATGACTTTTATCTGCTAGTAGAACCAGCAGTGGAGGTCCAAAAACCACTAACTGCTTCTTCCAAAACAATGTATTCTGCTTTGTTAATTCCCCGTAAGAAATAA", "aa": "MRAFGRVSTRIELIEKVFNDFYLLVEPAVEVQKPLTASSKTMYSALLIPRKK", "aa_hexdigest": "843a93a505593f211b12e21fab2f7c5d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5986.057400000001, "isoelectric_point": 9.867998313903808 }, "id": "GIHJHMOALN_303", "locus": "GCA009939745_00267" }, { "type": "cds", "sequence": "contig_1", "start": 151136, "stop": 151315, "strand": "+", "frame": 2, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02916", "RefSeq:WP_010874473.1", "SO:0001217", "UniParc:UPI0000133FF9", "UniRef:UniRef100_P75447", "UniRef:UniRef50_P75447", "UniRef:UniRef90_P75447" ], "nt": "ATGAAAGTAAAAAGTGCTGCAAAGAAGCGTTTTAAGCTCACTAAGTCTGGCCAAATTAAGCGCAAACACGCTTATACTTCCCACTTAGCACCGCACAAAACGACGAAGCAAAAGCGCCACTTGCGCAAGCAAGGAACTGTCAGTGCGAGTGACTTTAAACGCATTGGTAATTTAATTTAA", "aa": "MKVKSAAKKRFKLTKSGQIKRKHAYTSHLAPHKTTKQKRHLRKQGTVSASDFKRIGNLI", "aa_hexdigest": "5ed192a5600e39cb80ef8f07a769af7e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FF9", "ncbi_nrp_id": "WP_010874473.1", "uniref100_id": "UniRef100_P75447", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FF9", "RefSeq:WP_010874473.1", "UniRef:UniRef100_P75447" ] }, "ips": { "uniref100_id": "UniRef100_P75447", "uniref90_id": "UniRef90_P75447" }, "psc": { "uniref90_id": "UniRef90_P75447", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_P75447", "cog_id": "COG0291", "cog_category": "J", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75447" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "GIHJHMOALN_304", "locus": "GCA009939745_00268" }, { "type": "cds", "sequence": "contig_1", "start": 151326, "stop": 151709, "strand": "+", "frame": 3, "gene": "rplT", "product": "50S ribosomal protein L20", "db_xrefs": [ "COG:COG0292", "COG:J", "GO:0000027", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904", "RefSeq:WP_010874474.1", "SO:0001217", "UniParc:UPI0000133D19", "UniRef:UniRef100_P78023", "UniRef:UniRef50_Q7NBC0", "UniRef:UniRef90_P78023" ], "nt": "ATGAGAATTAAAGGTGGAAAACAAACCCGCGTACGTCGCAAGAAGTGGTTAAAGCAAGCTAGTGGTAGCTTTGGTACCCGACATGCCTCTTATAAGGTGGCAAAGCAAACGGTGATCCAAGCCGCTAAGTATGCTTACCGCGATCGTCGTAACAAAAAGCGCGATTTCCGTTCGTTGTGGATCTTGCGCTTAAACGCCGCTTTGCGCGAACAGGGCATGACCTATTCGGTCTTTATCAATCTATTGAAGAAGCACAACATTGAGATTAACCGCAAAGTGTTGTCGGAACTAGCCATTAAAGAACCAAGTAAATTTAACCTAATTGTGCAAAAGGTTAAAAGTGAACAACCGAAAGCCGCTAAGCCAGCTGCTTTAGGAAACTAG", "aa": "MRIKGGKQTRVRRKKWLKQASGSFGTRHASYKVAKQTVIQAAKYAYRDRRNKKRDFRSLWILRLNAALREQGMTYSVFINLLKKHNIEINRKVLSELAIKEPSKFNLIVQKVKSEQPKAAKPAALGN", "aa_hexdigest": "035eea5ede4fafe36c06d492b11e94cb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D19", "ncbi_nrp_id": "WP_010874474.1", "uniref100_id": "UniRef100_P78023", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D19", "RefSeq:WP_010874474.1", "UniRef:UniRef100_P78023" ] }, "ips": { "uniref100_id": "UniRef100_P78023", "uniref90_id": "UniRef90_P78023" }, "psc": { "uniref90_id": "UniRef90_P78023", "gene": "rplT", "product": "50S ribosomal protein L20", "uniref50_id": "UniRef50_Q7NBC0", "cog_id": "COG0292", "cog_category": "J", "go_ids": [ "GO:0000027", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q7NBC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7NBC0" ], "product": "Large ribosomal subunit protein bL20" }, "genes": [ "rplT" ], "id": "GIHJHMOALN_305", "locus": "GCA009939745_00269" }, { "type": "cds", "sequence": "contig_1", "start": 151684, "stop": 152394, "strand": "-", "frame": 1, "gene": "rnhC", "product": "Ribonuclease HIII", "db_xrefs": [ "COG:COG1039", "COG:L", "EC:3.1.26.4", "GO:0003723", "GO:0004523", "GO:0005737", "GO:0006298", "GO:0032299", "GO:0043137", "GO:0046872", "RefSeq:WP_010874475.1", "SO:0001217", "UniParc:UPI0000139442", "UniRef:UniRef100_P75446", "UniRef:UniRef50_P75446", "UniRef:UniRef90_P75446" ], "nt": "TTGCAACACGCAAACACAACCGCACTTTACCTAATTGGCAGTGATGAATCTGGCAAGGGTGATAGCTTTGGCGGAATTGCTGTAAGTGCTGTTTTAATTCATAAGGACAAAATCAATACACTCCACCAAATTGGTGTGGGGGATTCGAAGCAATTTAACGATTACCAAATTAAAGCTTTAGTGCCTAAAATCAAGGCAGCGGTTCATGACCAAGTTACATTAAGCGTTGATGCTAAAACTTACAACCAACTAGTGCAAAGCTTTAAAAATGTCAACGTAATGTTGACCTTTTTGCACTGCAAGGTGTACCACCAGTTGTTGCAACAAAACAAACTAACAGCACAACAATGTGACATTAGCATTGATGAGTTTGCTAATGTCAAACTGTTCACCCAATACACGCAAAAACTCACCAGCCTGCAAAATGAGTTAAAGGAATTAGTTATTCCCAACCACTTCTTAATTAGGGGTGAGAGCTATTCTAAGGTAATTGCCGCTGCTTCGATCTTAGCACGTGCTGCTTTTATTGCACAAATGGAACAGTTAAGCCACCAGTACGGTGTACAGTTTCCCAAGGGTAGTGCCCATGGCATTGTGGAAGCGTTACACTTACTAAAAACAAAAAGGCAGTTTCACAAGTTCGCCCAGTACAGTGCGGTCTGTAAAACTACCTTTAAGAATGTCGCTAGTTTCCTAAAGCAGCTGGCTTAG", "aa": "MQHANTTALYLIGSDESGKGDSFGGIAVSAVLIHKDKINTLHQIGVGDSKQFNDYQIKALVPKIKAAVHDQVTLSVDAKTYNQLVQSFKNVNVMLTFLHCKVYHQLLQQNKLTAQQCDISIDEFANVKLFTQYTQKLTSLQNELKELVIPNHFLIRGESYSKVIAAASILARAAFIAQMEQLSHQYGVQFPKGSAHGIVEALHLLKTKRQFHKFAQYSAVCKTTFKNVASFLKQLA", "aa_hexdigest": "a21daf5ea682856f414fb271068b4c4c", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139442", "ncbi_nrp_id": "WP_010874475.1", "uniref100_id": "UniRef100_P75446", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139442", "RefSeq:WP_010874475.1", "UniRef:UniRef100_P75446" ] }, "ips": { "uniref100_id": "UniRef100_P75446", "uniref90_id": "UniRef90_P75446" }, "psc": { "uniref90_id": "UniRef90_P75446", "gene": "rnhC", "product": "Ribonuclease HIII", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_P75446", "cog_id": "COG1039", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0032299", "GO:0046872", "GO:0003723", "GO:0004523", "GO:0043137", "GO:0006298" ] }, "pscc": { "uniref50_id": "UniRef50_P75446", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75446" ], "product": "Ribonuclease HIII" }, "genes": [ "rnhC" ], "id": "GIHJHMOALN_306", "locus": "GCA009939745_00270" }, { "type": "cds", "sequence": "contig_1", "start": 152450, "stop": 153175, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00226.36" ], "nt": "ATGGCAGAGGCAAAACGTGATTATTATGAAGTCTTAGGGCTTAGTCGGGATGCTGATGATAACGACATAAAAAAGGCCTTTCGTAAACTAGCCAAAAAGTACCACCCCGACCGCAATAAGGCACCGGATGCGGCTCAAATTTTTGCCGAAATTAACGAGGCCAATGACGTGTTGTCTAACCCCAAAAAACGTGCTAACTATGACAAATACGGCCATGATGGGGTGGACAATGAAGGTGGTTTTGCCTTTCAAGCTGATGTGTTTGATTCTTTTTTTGAGGAGATTGAAAAATCGGGTGCCTTTGATAATTTAAGTGAGTCTAACACCAAAAAGAAAGAGAAGACTAAAACTAAGAAGAAGGGTTGGTTTTGGGGCAAATCCAAACAGGAAGAATCTACCAGCGATACTACTGAATATGCCGATGTTGACGCTGGTTTAGAAGATTATCCGCCTCAATCTGATTATCCAGATGATATTCCTGATGTGGATGCCAGGATCGAAGAAGTGGATCAATCTGCCTATGCCGATGACATTCCCGATGTGGATGCGGGAATGGATTGGGAACAAAACGCAGAAGTAGCAAATTCTGCTAGTGAAATCATCCCCGATGTCGATGCTGGTTTAGCGGATGAGTTTAATACTAGTAGTGCAGCACCCCAAGCTAGTGACTGGGAAGCAATGATTGGTAATCCTGAGTACGGTTACTTTGATGCTGCTGGTGAGTGA", "aa": "MAEAKRDYYEVLGLSRDADDNDIKKAFRKLAKKYHPDRNKAPDAAQIFAEINEANDVLSNPKKRANYDKYGHDGVDNEGGFAFQADVFDSFFEEIEKSGAFDNLSESNTKKKEKTKTKKKGWFWGKSKQEESTSDTTEYADVDAGLEDYPPQSDYPDDIPDVDARIEEVDQSAYADDIPDVDAGMDWEQNAEVANSASEIIPDVDAGLADEFNTSSAAPQASDWEAMIGNPEYGYFDAAGE", "aa_hexdigest": "b22a96e26c130cdba3c187414383c4f4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00226.36", "name": "DnaJ", "length": 63, "aa_cov": 0.2572614107883817, "hmm_cov": 1.0, "evalue": 1.3730986984720544e-27, "score": 91.14558410644531, "start": 7, "stop": 68 } ], "seq_stats": { "molecular_weight": 26822.45639999997, "isoelectric_point": 4.249249076843263 }, "id": "GIHJHMOALN_307", "locus": "GCA009939745_00271" }, { "type": "cds", "sequence": "contig_1", "start": 153371, "stop": 154123, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACCGGAAACTGAACCAGAACCAATTATTACCTTAAGTTCAGAGCCAGTGGAAGCACCAGCTTCTGTTGTGATTGAACCTACCCCTGAAACTGAGGAAACAACTAGTGCTGTAGAAATGGATGCTTCAGTTAAAGCAGATGTTTCTGATGAAGCTGACGCAACTAATGAACCAACTGAACAGGATACCATTTCAGAGCCAGAACAAGAAACAGACGCGGCTGCATTAGAAGAAATTAACCACACCACTGCTGATTTGGAACCAGCAGAAGTTAGTGCTACTAATGATCTAGAACAAGATGTAGTAGAGAAAGTTAATTTTTCAGAACCAGAATCAACAGTAGATACTGCTGCAACCGATCCTGTTGTAGAACAAGCTACGGAAACCTCCACCAATGGTTTCAAATTCTTTAACTTTAGTTCGTTTGTTTTAAGTGACCAAAACCCCAATCCACAAACACCCACCCACCATGAAGAAGATGCTGCAGCCCCCGAGCCAACCGTTGATGAAACTTCTGGAGAATCCACAGCCCCCGAAGTTACCATAGCTGAGTCAACTGTAGAATTGGAAACCGCAGCGGAAATTAATAACCCCGCTACCTTTGTGGAAGAATACCTCCAACCAACTAAAACTACGGTTGTTGATAAATTAGATGAACCAACTGTAGCAAAACCTACTGTTAGCGATTCTGAAAATTCAGTTGCACCAGAACCGGAATTTGTTGCTGGACCGGAACAAACCTTTAGCTGA", "aa": "MQPETEPEPIITLSSEPVEAPASVVIEPTPETEETTSAVEMDASVKADVSDEADATNEPTEQDTISEPEQETDAAALEEINHTTADLEPAEVSATNDLEQDVVEKVNFSEPESTVDTAATDPVVEQATETSTNGFKFFNFSSFVLSDQNPNPQTPTHHEEDAAAPEPTVDETSGESTAPEVTIAESTVELETAAEINNPATFVEEYLQPTKTTVVDKLDEPTVAKPTVSDSENSVAPEPEFVAGPEQTFS", "aa_hexdigest": "99b832c60caa1eb0ae0d7749e70ec51e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26726.076700000005, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_308", "locus": "GCA009939745_00272" }, { "type": "cds", "sequence": "contig_1", "start": 154571, "stop": 155182, "strand": "+", "frame": 2, "gene": "dnaJ", "product": "DnaJ C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557D12" ], "nt": "TTGAACGAACAGCTCAAGGAAATTAGGTACACCCGTAAGTTAGTCGATCCACAAACTCAGGTAACCACCACTGAATCAATTACCCTAGAAGTTCAGCTATCACATAAATCCCAAACGGAAGCAATTGCCATCTTTAAGGGCTTTGGTCATGACTATGGTAGTGGCTGTGGTGACCTTAAGGTAGTACTTAAAGTGATCCCTAGTGTCTTTTTCCAACTGCAAGCTGATGGCTTACACACCGCTGCCCTAGTCGACCCTCTAGTGGCTTACAACGGTGGTTTAATTGATTTCTTTGGTCCAGTTAACAGCTTTAAAGTTGATATTGAAGGTGGGATTGCTAACAACGATTTAATTGAGTTTAACCAGTTAGGAGTACTAAGAACTAAGACCAAACGTGGTAGTTTATACGTCCACTTGTACTATAGTAGTGTAGCCAAAAAGGGCACTAAGACTAACTGCCAAGTGCAACAGTTCTTTGATCTAGTTCATGTGGAATACAAGCTGTTAAACTACAACTTAAAACAACTCCACAACTACCACTCGGCTCTCACTGCCCAGAAAAAAACCTTGGACCGCAAAAGCTATCAATGTTTAGCAGTGGAAAGCCATTAA", "aa": "MNEQLKEIRYTRKLVDPQTQVTTTESITLEVQLSHKSQTEAIAIFKGFGHDYGSGCGDLKVVLKVIPSVFFQLQADGLHTAALVDPLVAYNGGLIDFFGPVNSFKVDIEGGIANNDLIEFNQLGVLRTKTKRGSLYVHLYYSSVAKKGTKTNCQVQQFFDLVHVEYKLLNYNLKQLHNYHSALTAQKKTLDRKSYQCLAVESH", "aa_hexdigest": "9c19cea1e031089383b878fc031ac15a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557D12", "query_cov": 0.9014778325123153, "subject_cov": 0.9838709677419355, "identity": 0.536, "score": 193.0, "evalue": 1.92e-59, "valid": false, "product": "DnaJ C-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI0000557D12" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557D12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557D12" ], "product": "DnaJ C-terminal domain-containing protein" }, "genes": [ "dnaJ" ], "id": "GIHJHMOALN_309", "locus": "GCA009939745_00273" }, { "type": "cds", "sequence": "contig_1", "start": 155205, "stop": 155657, "strand": "+", "frame": 3, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "GO:0000774", "GO:0005737", "GO:0006457", "GO:0042803", "GO:0051082", "GO:0051087", "KEGG:K03687", "SO:0001217", "UniRef:UniRef50_P47443", "UniRef:UniRef90_P78017" ], "nt": "ATGAGCGAAAACTCACTCACAATTACCGAAATTCTCAGTTCCATCCGTACGCTTTTAGTCAAACACAATAAGGCTAAAGTAACCCAAATTGAAAAGGAACTTTTACAAGCAGTTGCTGAACTGGAAAAGAAGTTTAAGCAACAAGTACAAAACTTCAACGAGTTGCAACAAAAGATTCCTAACTTACAAAAAGTCAACGAGGAATTTCGCTTAAAAGTGGAAAAAATCCAGGAAGAAGCGCAGAAGAAAATTCAGGAAAAAGTGGCAGAACTGACCATTAAAAGTAAGGAAGAGTTGGAAAATGCAAAGAAGTATGTGATCGAAAAGTCGATTGATCAACCCTTAATTATTATTGACCAGTTCGAAATTGCACTTTCCTATGCTCAAAAGGATCCCCAAGTAAAAAACTATACCACTGGGTTTAACATGGTGTTGGATGCCTTTTCGCGCTGA", "aa": "MSENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIPNLQKVNEEFRLKVEKIQEEAQKKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQFEIALSYAQKDPQVKNYTTGFNMVLDAFSR", "aa_hexdigest": "b4cb6dcd106da9db7a74149b5bbc8cc3", "start_type": "ATG", "rbs_motif": null, "genes": [ "grpE" ], "truncated": "3-prime", "pseudogene": { "start": 155205, "stop": 155858, "observations": { "mutation": [ 155655 ], "stop": [ 155655 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78017", "query_cov": 1.0, "subject_cov": 0.6912442396313364, "identity": 1.0, "score": 266.0, "evalue": 6.31e-89, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MSENSLTITEILSSIRTLLVKHNKAKVTQIEKELLQAVAELEKKFKQQVQNFNELQQKIPNLQKVNEEFRLKVEKIQEEAQKKIQEKVAELTIKSKEELENAKKYVIEKSIDQPLIIIDQFEIALSYAQKDPQVKNYTTGFNMVLDAFSRWLEGFGVTKIAIEPGAQFDEKVMAALEVVPSDQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE" }, "paralog": false, "identity": 0.9907834101382489, "score": 407.0, "evalue": 7.55e-147, "description": "Internal stop codon at 155655. Nonsense mutation around 155655.", "psc": { "uniref90_id": "UniRef90_P78017", "gene": "grpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_P47443", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687", "go_ids": [ "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78017", "UniRef:UniRef50_P47443", "COG:COG0576", "COG:O", "KEGG:K03687", "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ] }, "pscc": { "uniref50_id": "UniRef50_P47443", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47443" ], "product": "Protein GrpE" } }, "id": "GIHJHMOALN_310", "locus": "GCA009939745_00274" }, { "type": "cds", "sequence": "contig_1", "start": 155721, "stop": 155858, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCACTGGAAGCTGTGCCTTCTGATCAACCGGCTAATACGGTGGTGAAGGTATCGAAATCGGGTTACAAACTCCACGACAAGGTCATTCGCTTTGCTTCCGTAGTGGTAAGCCAAGGTAATAAAACAGAATAA", "aa": "MAALEAVPSDQPANTVVKVSKSGYKLHDKVIRFASVVVSQGNKTE", "aa_hexdigest": "30c7f6f32836c5f5415e21333296a8de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4800.448800000002, "isoelectric_point": 9.302868461608885 }, "id": "GIHJHMOALN_311", "locus": "GCA009939745_00275" }, { "type": "cds", "sequence": "contig_1", "start": 155858, "stop": 156223, "strand": "+", "frame": 2, "gene": null, "product": "MPN121 family protein", "db_xrefs": [ "RefSeq:WP_010874478.1", "SO:0001217", "UniParc:UPI0000139461", "UniRef:UniRef100_P75353", "UniRef:UniRef50_P75353", "UniRef:UniRef90_P75353" ], "nt": "ATGAGCACAAATAAAAGAAGAACCATTCAGATTGAAATCACTGAAGAACACTTCAAGGATTTAGAAAAAGCCTTGGAAGCCTTGAAGGGTACCCAATTACCCTTCTCCACTACCGTAGAACAGTTTGTGGAATTAATTTTGTCCAACTATGTCGCTACTTCCAACAAGATTTCTAATTTAGCTGAAAGTGGCTTTGATGTGGCTTCTATTCAACAAGAATTAGAAAAAATTGGCAGCGCTGCAGGGGCGGATGATGCGCTCAAAAGCTTCTTGGATGAGTTGTTAAAAACATCGCAAAAAAGCTTTAGCAATACCAAAGATGGCAAAAAAAATGATGACGATAATAATTCGTCATCAAAATCATAA", "aa": "MSTNKRRTIQIEITEEHFKDLEKALEALKGTQLPFSTTVEQFVELILSNYVATSNKISNLAESGFDVASIQQELEKIGSAAGADDALKSFLDELLKTSQKSFSNTKDGKKNDDDNNSSSKS", "aa_hexdigest": "58ad45def04906f3e98d12365a0a2c20", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139461", "ncbi_nrp_id": "WP_010874478.1", "uniref100_id": "UniRef100_P75353", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139461", "RefSeq:WP_010874478.1", "UniRef:UniRef100_P75353" ] }, "ips": { "uniref100_id": "UniRef100_P75353", "uniref90_id": "UniRef90_P75353" }, "psc": { "uniref90_id": "UniRef90_P75353", "product": "MPN121 family protein", "uniref50_id": "UniRef50_P75353" }, "pscc": { "uniref50_id": "UniRef50_P75353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75353" ], "product": "Uncharacterized protein MG202 homolog" }, "genes": [], "id": "GIHJHMOALN_312", "locus": "GCA009939745_00276" }, { "type": "tRNA", "sequence": "contig_1", "start": 156241, "stop": 156314, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 62.8, "nt": "GCCGAATTAGTTTAGGGGCAAAACAGATCCATGGTAAGGATCAGAGAACAGTTCGACTCTGTTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 156280, 156282 ], "id": "GIHJHMOALN_33", "locus": "GCA009939745_00277" }, { "type": "cds", "sequence": "contig_1", "start": 156349, "stop": 158004, "strand": "+", "frame": 1, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47445", "UniRef:UniRef90_P78016" ], "nt": "ATGAAAAATAATTACAGCGAAGCGAATATCAAAATTCTCAAGGGGCTAGATGCTGTAAAAAAACGCCCGGGAATGTACATCGGCTCAACCGATAGTCGCGGTTTTCACCACTTGCTGTGGGAAATTTTAGACAACTGTGTCGATGAAGTTTTATCGGGCTTTGCTAATACTATTGCCGTTGTTTTACATGCCGAAAACCAGATAACTGTTAGTGACAACGGTCGTGGTATTCCCTTCGAAACACACTCCGATTCCAAGATTTCTACGATTGACACCGTCTTTACCTACCTCCATGCCGGTGGTAAGTTTGATAACGATTCTTATAAGATTGCTGGAGGGTTGCACGGGGTGGGGGCTTCGGTGGTGAATGCCTTGAGTGACCAGTTACAAGTAACGGTTAAACGTCAGGGCAAGGTGTACCGCTCGGTGTATGAAAATGGTGGTAAAATTAAGCAAAAAGCGCATTGCATTGGTAATGCCAAAATTGACGAACACGGTACCAGTGTGACTTTTCGTCCTGACCCAAAGGTCTTTAAAAAGATCCACTTTGACAGCGAACTAATTCGTGCGCGGTTAAAGGAATTAGCCTTCCTTTTCAAAAAGTTACAACTGACCTTCGTTGATGAAACCGGCAGTGGGGAAAAAGAGGTGTTCTTTACCGAAGCCGGTATTAGTCAGTACTTGGACGAACTTAACGCTGATTCCAAACAAATTGCGCAAAAGATCTTTGTTAGTGGCACTGAAGATGACATTGAACTGGAAGCCGTGTTTCAGTTTATTGATGGGGAGGATGAAAAACTACTTTCGTTTGCCAATTCGATTCGCACCAGCGAAGGGGGTAGTCATGAAGCGGGTTTTCGTCAATCTGTTGGTGATGTGATTAACAACTACTGCCGCAAGTACAACTTCCTAAAGGAGCGTGACAAGAACTTTGAGGCCAGTGAAATCCGTGAAGGCTTAAACGGCATTATTAAGGTCAATCTCCCCGAAAAGATTATTGCCTTTGAAGGGCAAACGAAGAGCAAACTGTTCTCCAAGGAAGTTAAAGCGGTAGTGCAAAAGCTAACCCAAAAGCACTTCTTCCAGTTCTTGGAACGTAACAGTGTTGATGCTAAATTAATTGTCGAAAAGCTGTTTTATAACCGCAAACTGCGCCAAGAACTCAAACAACAACGCCAAATTAAAAAGAACCTTTCTAATCCGAAGGCCGAGCGCATTTTGTTTGGTAAATTAGCACCGGCGCAGTCCAAAAAAGTGGCTGAACGCGAACTGTTTGTGGTGGAAGGTGACAGTGCTGGTGGTACGGCTAAAATGGGACGAGATCGCTTTCTACAAGCCATCTTACCGTTACGCGGTAAGGTGTTAAATGTGGAGAAAATTAACAACAAGAAGGAAGCGATTAACAACGAAGAGCTGTTAACACTCATCTTCTGTATCGGTACTGGTATTGGTAACAACTTCACCATTCGCGATCGTAAATACGACAAGATTATTATCATGACTGATGCTGACAATGACGGTGCGCACATCCAGATTTTACTGCTCACCTTCTTCTACCGTTACATGAAACCCCTAATTGAAAAAGGACACATTTACCTAGCACTTCCACCACTTTATAAGTTTGAAGGTCGTGATAAAAAAGCCCGTTATCTCTGA", "aa": "MKNNYSEANIKILKGLDAVKKRPGMYIGSTDSRGFHHLLWEILDNCVDEVLSGFANTIAVVLHAENQITVSDNGRGIPFETHSDSKISTIDTVFTYLHAGGKFDNDSYKIAGGLHGVGASVVNALSDQLQVTVKRQGKVYRSVYENGGKIKQKAHCIGNAKIDEHGTSVTFRPDPKVFKKIHFDSELIRARLKELAFLFKKLQLTFVDETGSGEKEVFFTEAGISQYLDELNADSKQIAQKIFVSGTEDDIELEAVFQFIDGEDEKLLSFANSIRTSEGGSHEAGFRQSVGDVINNYCRKYNFLKERDKNFEASEIREGLNGIIKVNLPEKIIAFEGQTKSKLFSKEVKAVVQKLTQKHFFQFLERNSVDAKLIVEKLFYNRKLRQELKQQRQIKKNLSNPKAERILFGKLAPAQSKKVAERELFVVEGDSAGGTAKMGRDRFLQAILPLRGKVLNVEKINNKKEAINNEELLTLIFCIGTGIGNNFTIRDRKYDKIIIMTDADNDGAHIQILLLTFFYRYMKPLIEKGHIYLALPPLYKFEGRDKKARYL", "aa_hexdigest": "31e29ceb8f1bad27701229cbeb646df2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P78016", "query_cov": 1.0, "subject_cov": 0.8677165354330708, "identity": 0.998, "score": 1073.0, "evalue": 0.0, "valid": true, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47445", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47445", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47445" ], "product": "DNA topoisomerase 4 subunit B" }, "genes": [ "parE" ], "id": "GIHJHMOALN_313", "locus": "GCA009939745_00278" }, { "type": "cds", "sequence": "contig_1", "start": 158017, "stop": 158256, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_T0TWZ4" ], "nt": "TTGGAACAGTACCGTGCTAAGCATAGCCACTTTAATGTGCAACGGTACAAGGGTTTAGGGGAAATGAATGCTGATCAGCTCTGGGAAACCACGATGAACCCGATGACGCGTAAACTAATTCAGGTCAAATTGGATAACTTTATCCAAGCTGAAAAGCAAATTAATGTTTTTATGGGTGATAAAACCGAATTGCGTAAAAGCTGGATTGAAGCTAACATTAACTTTAGTAGTGAGAACTAA", "aa": "MEQYRAKHSHFNVQRYKGLGEMNADQLWETTMNPMTRKLIQVKLDNFIQAEKQINVFMGDKTELRKSWIEANINFSSEN", "aa_hexdigest": "5b4366a3a2c34657f08d4ad6e69b2d63", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1Q6V0H2", "query_cov": 0.8607594936708861, "subject_cov": 0.8395061728395061, "identity": 0.618, "score": 91.3, "evalue": 1.35e-22, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_T0TWZ4" }, "pscc": { "uniref50_id": "UniRef50_T0TWZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_T0TWZ4" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "GIHJHMOALN_314", "locus": "GCA009939745_00279" }, { "type": "cds", "sequence": "contig_1", "start": 158256, "stop": 158543, "strand": "+", "frame": 3, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "nt": "ATGGAAAAGAATAAACAAGCGTTATTACTCCAGGCCATCGAAGATGTCTTTGCCTTTAGCTTTAGTAAGTATGCTAAGTACATTATCCAAGACCGGGCTTTACCTGATCTACGTGATGGGCTTAAACCAGTTCAACGCCGCATCCTCTATGGCATGTACCAAATGGGCTTAAAACCCACTTCACCCTACAAAAAGTCAGCTCGGGCAGTCGGAGAAATTATGGGTAAGTACCACCCCCATGGCGATGCATCGATCTATGATGCGATTGTAAGGATGTCACAAGCCTGA", "aa": "MEKNKQALLLQAIEDVFAFSFSKYAKYIIQDRALPDLRDGLKPVQRRILYGMYQMGLKPTSPYKKSARAVGEIMGKYHPHGDASIYDAIVRMSQA", "aa_hexdigest": "5a748c3ede98040626375442c755abc5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028540D82", "query_cov": 0.8736842105263158, "subject_cov": 0.83, "identity": 0.6629999999999999, "score": 124.0, "evalue": 3.5e-35, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_Q9XCU2" }, "pscc": { "uniref50_id": "UniRef50_Q9XCU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "GIHJHMOALN_315", "locus": "GCA009939745_00280" }, { "type": "cds", "sequence": "contig_1", "start": 158613, "stop": 160625, "strand": "+", "frame": 3, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335", "SO:0001217", "UniRef:UniRef50_P47446", "UniRef:UniRef90_P75352" ], "nt": "ATGCGCTATACGGAAGCGCGTTTAAGTCCGTACGGGTTTGAACTACTCAAAGACATTGAAAAGCAGTTAGTACCGTTTGTGAATAACTTTGATGACAGTGAGGTCGAACCGAGCGTGTTACCAACCCTGTTACCTAACCTCTTTATTAACGGTACCAGTGGAATAGCGGCGGGTTATGCCACTAATATTGCACCCCATAATGTCGGTGAACTGCTTGATGGTTTGAGTTACCGGATTGAAAACCCTGATTGTGATTTAAAGGCCATCTTAAAGATTGTTAAGGGACCGGATTTTCCAACTGGTGGATTAGTGTACTTTGAGCAAGAGTTGGCGAACATCTACCAAACTGGGAAGGGTAAGTTTGTGATTCAGGCCAAATATGAAACGAACACTGCGTTTGGCCAAAACCAGATTGTAATCACCGAAATCCCATACGAAACGGTGAAGGCCAATATTGTTAAACAAATTGAAGAGTTAATTAGTGACAATAAACTCAGTGCCTTGGAAAGTGTGATTGACAGCTCTGATCGTAGTGGTATTCGGATCATTATTAACCACAAGGACTTTTTGAGTGCTGACAAGATTATGGCGTTCTTGTTCAAACACACCCAACTCCAAGTAAACTTTAACCTCAACAATACGGTAATTGCCAACCGTTGTCCGGTACGGGTGGGGTTGTTAGCTTACTTTGATCAGTTCCTAGCCTTTGCCCACGAATTGATCATTAACAGCGCCAAGTATGATTTAGCATTGGCCAATAAGCGCTTGGAAATCATTAAGGGCTTAATTAAGGCGGTTTCGATGATTGATGAAATTATTCGTTTAATTCGCCGTGCTACTGACAAACAGGATGCGAAAACCAAGCTCATTGACAAGTATGCTTTTACCCTAAATCAAGCCGAAGCGATTGTGTCGTTGCGTTTGTACCAGTTAACTAACACCGACATTAAGGTCCTGTTTGCCGAACAAAAAGAGTTAGAGCAAACGATCCAAACAGCCGAATGCTTAATTGCTAAACCGCAAGCACGAAACCAGTTGTTACAAGCACAGTTTTTCCAATACAAAAAGCAGTTTAACCAACCACGACGCGCACAAATAGTTGGTTTAATTGAAAAGCAAAAGGTACAAGACAGTGACTTCATCGAACACAAGGAAGTGGGCTTACTCATTAGTCACGATGGCATCTACTTTAAGTTTGAACCAGAACAGTTAGCGAAGCACCTAGTGGAATTTAAAAGTGAACAAGACCAACTGATTTTTGGAGGCGTAGTACAAAACTCCGATTACTTTTTTATGGTGACTTCACTCGGTAACATTATTACCGTTCCCATTTACAAAACGTTAAGTAACACCAAAACCAAGATGAACGAGTTGTTGGCCAAAAAACCGATCCTAATGGAAGATGAAAAATTGGTACTAGCAGGGATTGTAAACCCAGACAAAATGGAACAACAGCTCTTGGTGTTAACGTCACAGTGCGGTATGGTCAAACGGGTCGAGTTGAGCAAAGTCATTAACACGAAGCAAATTAAGTCAAGCTGCTGCATGGCACTCCGGGAACGCGACAAACTGGTCAACGCCTTTGTTCAAACTAAAAGGGAACCTAAACTAGTTTGTCTGGTGTCATCTTCCAACAGTTTTGCTACCTTCTTAGCGGAAGAAATTCCAATTATTAGTAACAAGGGAATTGGGGTGAAGGGGATTAAACTCAAAGCTGAGGAAAAGGTACGGTTTGCAATGCCGTTACAGGATAATGATGCCTTAGTAGTGATTAACAGTGATGGTGGTGTCTATAACTTTGAAGTAGTGGAACTGGCAGTCGCTAGCCGCATGAGTGTTGGTAAAAAACTAATTCCCAAAACCAAAACCCCGGTAAGCTGTTTTGCTGCTAATAAGCACAGTGAGATTATTGGTCATCGTGGTAAAAATGGTAGTGATCTGTTTACCCTAAATGAGTTAAACCGTTTACCAAAGTCAACCGTTAGTCAAATGCGTTTGTTTAAGTGGAGCTAG", "aa": "MRYTEARLSPYGFELLKDIEKQLVPFVNNFDDSEVEPSVLPTLLPNLFINGTSGIAAGYATNIAPHNVGELLDGLSYRIENPDCDLKAILKIVKGPDFPTGGLVYFEQELANIYQTGKGKFVIQAKYETNTAFGQNQIVITEIPYETVKANIVKQIEELISDNKLSALESVIDSSDRSGIRIIINHKDFLSADKIMAFLFKHTQLQVNFNLNNTVIANRCPVRVGLLAYFDQFLAFAHELIINSAKYDLALANKRLEIIKGLIKAVSMIDEIIRLIRRATDKQDAKTKLIDKYAFTLNQAEAIVSLRLYQLTNTDIKVLFAEQKELEQTIQTAECLIAKPQARNQLLQAQFFQYKKQFNQPRRAQIVGLIEKQKVQDSDFIEHKEVGLLISHDGIYFKFEPEQLAKHLVEFKSEQDQLIFGGVVQNSDYFFMVTSLGNIITVPIYKTLSNTKTKMNELLAKKPILMEDEKLVLAGIVNPDKMEQQLLVLTSQCGMVKRVELSKVINTKQIKSSCCMALRERDKLVNAFVQTKREPKLVCLVSSSNSFATFLAEEIPIISNKGIGVKGIKLKAEEKVRFAMPLQDNDALVVINSDGGVYNFEVVELAVASRMSVGKKLIPKTKTPVSCFAANKHSEIIGHRGKNGSDLFTLNELNRLPKSTVSQMRLFKWS", "aa_hexdigest": "c0e9c81703a425db3fd07f8fe110609c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75352", "query_cov": 1.0, "subject_cov": 0.8491761723700887, "identity": 0.997, "score": 1283.0, "evalue": 0.0, "valid": true, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47446", "cog_id": "COG0188", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47446", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47446" ], "product": "DNA topoisomerase 4 subunit A" }, "genes": [ "parC" ], "id": "GIHJHMOALN_316", "locus": "GCA009939745_00281" }, { "type": "cds", "sequence": "contig_1", "start": 160612, "stop": 160863, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCTTTATGAACCAAAACAACCAGACTACTAATATTACGTTTGGTGACAAGTTAGGATTAGAAGGGGAAGAGGTTTCGGTAGCTTCTACTCTTATTAATACCACCAACGAAAGTAAGCACCAACTAGCGATAGTGGGTCCCACCCGGATGGACTACCAAAAGGTGAAAGCGTTATTGTTGACCCTCAAAGAAGAGATCGAGGAGTACGATAAGCAACTTCACGGTGGTAAAACCACTAGCTCCACTTAA", "aa": "MAFMNQNNQTTNITFGDKLGLEGEEVSVASTLINTTNESKHQLAIVGPTRMDYQKVKALLLTLKEEIEEYDKQLHGGKTTSST", "aa_hexdigest": "6158fecdc70c3a4303ea66eef2e7d5af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9202.240299999998, "isoelectric_point": 5.237963294982909 }, "id": "GIHJHMOALN_317", "locus": "GCA009939745_00282" }, { "type": "cds", "sequence": "contig_1", "start": 160870, "stop": 161667, "strand": "-", "frame": 1, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "GO:0045892", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_P75351", "UniRef:UniRef90_P75351" ], "nt": "ATGAAAAATTTAACTACCCGACAAGCCCAAATTCTCAAGGCCATCATCAACGAATATATTGCCTATCCAGTACCTGTTGGTAGTAAATTATTAACTAAAAAATATTTTAAAAACCTCTCTGGCGGTACACTCCGAAACGAAATGGCCGTTTTGGAAAAAGAGGGTTATCTCAAAAAGAACCACATCTCTTCAGGACGAATCCCCTCCCAGTTGGGCTATCAGTACTATGTCAAGTTACTCACTAAAAATGATGATAAGAGTAACCTTAAAACCCGCTTAAGGGCGATTATCTTGCAAAAACACAAAACAATTGATGAAATTATTGAACTGGGGGTAAAGTTTATCAACGAAATGGTAAACCTACCAGTGGTGTTAACCCACTTTAGTAGTGATGAAGTCTTAAAAAAGATTGATCTCATTATGCTCGATCAGTCCTGTGCACTGCTACTGTTAGTGTCAGCTAGTGGTAACGTCTTTAAGAAAACGATTAGCTATGCTAACCAAAGACAGTTTGAAGACATCATGGTATGTGTCAGGATCTTTAATGATCGCATTATCGATACACGTTTCTGTGATATTGCCCAACACTTAGATGTTTTAAAGGAAATTATTCGCAGCAAGGTACACGAGTACCAGTATGTCATTGACGAAATCCTGTTTAAGCTGTTTAACTTTGAGGAATTCCAACAAGCACGTAAGCAGGTTTATGGCATCCATTACCTAGCCCAACAACCAGAGTTTGCCAACCAGGAAAGATTGACGCGTATCTTAAACTTACTGGAAGATACTAGTGTCTGA", "aa": "MKNLTTRQAQILKAIINEYIAYPVPVGSKLLTKKYFKNLSGGTLRNEMAVLEKEGYLKKNHISSGRIPSQLGYQYYVKLLTKNDDKSNLKTRLRAIILQKHKTIDEIIELGVKFINEMVNLPVVLTHFSSDEVLKKIDLIMLDQSCALLLLVSASGNVFKKTISYANQRQFEDIMVCVRIFNDRIIDTRFCDIAQHLDVLKEIIRSKVHEYQYVIDEILFKLFNFEEFQQARKQVYGIHYLAQQPEFANQERLTRILNLLEDTSV", "aa_hexdigest": "53d5a70a4a46a8ca087e08370db515f7", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 160612, "stop": 161667, "observations": { "mutation": [ 160872 ], "stop": [ 160872 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75351", "query_cov": 1.0, "subject_cov": 0.7549857549857549, "identity": 1.0, "score": 510.0, "evalue": 2.98e-181, "gene_start": 1, "gene_end": 265, "reference_start": 1, "reference_end": 265, "reference_sequence": "MKNLTTRQAQILKAIINEYIAYPVPVGSKLLTKKYFKNLSGGTLRNEMAVLEKEGYLKKNHISSGRIPSQLGYQYYVKLLTKNDDKSNLKTRLRAIILQKHKTIDEIIELGVKFINEMVNLPVVLTHFSSDEVLKKIDLIMLDQSCALLLLVSASGNVFKKTISYANQRQFEDIMVCVRIFNDRIIDTRFCDIAQHLDVLKEIIRSKVHEYQYVIDEILFKLFNFEEFQQARKQVYGIHYLAQQPEFANQERLTRILNLLEDTSVWQQMAFMNQNNQTTNITFGDKLGLEGEEVSVASTLINTTNESKHQLAIVGPTRMDYQKVKALLLTLKEEIEEYDKQLHGGKTTSST" }, "paralog": false, "identity": 0.9971509971509972, "score": 682.0, "evalue": 3.54e-251, "description": "Internal stop codon at 160872. Nonsense mutation around 160872.", "psc": { "uniref90_id": "UniRef90_P75351", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_P75351", "cog_id": "COG1420", "cog_category": "K", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355", "GO:0045892" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75351", "UniRef:UniRef50_P75351", "COG:COG1420", "COG:K", "KEGG:K03705", "GO:0003677", "GO:0006355", "GO:0045892" ] }, "pscc": { "uniref50_id": "UniRef50_P75351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75351" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "GIHJHMOALN_318", "locus": "GCA009939745_00283" }, { "type": "cds", "sequence": "contig_1", "start": 161792, "stop": 162781, "strand": "+", "frame": 2, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_P47448", "UniRef:UniRef90_P75350" ], "nt": "TTGTATGTCGGGAAGGCTAAGGACATCTTTAAGCGGGTACACCATTACTTTAATCCAAACCGTTCCTTTAAAACACGGGCACTAGTGGAGCGAATTGCGGATGTCGAATACGTCATTTTGAAAAATGAAAATGATGCTTTGAACTTAGAGGCCAAACTAATTAAACAGTACAAACCGCGCTTTAATTTAGTTTTAAAGGAAAATAATGGTTATCTCTATTTCTTTATTACCGCCAGTGTTAAACCCACCTTGGAATTGGGCCGCCGGTATGAGTTTTCCAAGAATAAGTACTTCGGTCCGTTTGCATCGAGTAAGTTTCGACTGCGTGACATTTATGACCTGTTGTTAAAGCTTTTTCCATTACGCAAGTGCGCGCCCCATGAACGGGGTCACCCCTGCTTTTACTATCAGCTTAAAATGTGCATGGGTCAGTGTATGGGCGAGGATACACCGGAACGTTACCAGACCACCGTCAAGGGAATTGAACAGTTTTTTAACCACGGTCCCGAGCAGGTTTTAAACCACTTGCAACAGCAAGAAATCAAGGCTAGTGAACAACAAAACTTTGAAGCAGCACGCCACTTCTTGGACTTACAGAAAGCCGTTTTAGAACTAGTGAACATGCAACAAACGGCCTTCATTAAAGCAAAGCAAAGCCATGACTTTATTGGTTATGTCTTTGAGAAAAATGTGTTAGCCATTACCGTCTTTGCTTACGTTGATAACCAACTAATAGGCAAAAACCAGCAGGTCTTTGAACTACCACAGGACGATGAAAAGGAAGTTGAAAGTGCTTTGGTTACCTTTATTTACCACTATTATTCCACCAATAAGATACCCAAAACCCTTACAGTTTCTTTAAGCGAAGAGAATTTGAGCTTACTGGCCAACAGTCTTAAAATTAATGTTACGCAACCAAAAAACGGCGAGCAAAAATCCATTTTGCAAACCGTGATTGACAACGCCCGTTATGCCTTAAACACCAAGTGA", "aa": "MYVGKAKDIFKRVHHYFNPNRSFKTRALVERIADVEYVILKNENDALNLEAKLIKQYKPRFNLVLKENNGYLYFFITASVKPTLELGRRYEFSKNKYFGPFASSKFRLRDIYDLLLKLFPLRKCAPHERGHPCFYYQLKMCMGQCMGEDTPERYQTTVKGIEQFFNHGPEQVLNHLQQQEIKASEQQNFEAARHFLDLQKAVLELVNMQQTAFIKAKQSHDFIGYVFEKNVLAITVFAYVDNQLIGKNQQVFELPQDDEKEVESALVTFIYHYYSTNKIPKTLTVSLSEENLSLLANSLKINVTQPKNGEQKSILQTVIDNARYALNTK", "aa_hexdigest": "0bb300c07fadff740364e38d732f90b3", "start_type": "TTG", "rbs_motif": null, "genes": [ "uvrC" ], "truncated": "both", "pseudogene": { "start": 161699, "stop": 163084, "observations": { "mutation": [ 161768, 162779 ], "stop": [ 161768, 162779 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75350", "query_cov": 1.0, "subject_cov": 0.5483333333333333, "identity": 0.997, "score": 619.0, "evalue": 4.72e-219, "gene_start": 1, "gene_end": 329, "reference_start": 32, "reference_end": 360, "reference_sequence": "MIIVNNTLAFKLKNAPHKPGCYLWKDDAGQVLYVGKAKDIFKRVHHYFNPNRSFKTRALVERIADVEYVILKNENDALNLEAKLIKQYKPRFNLVLKENNGYLYFFITASVKPTLELGRRYEFSKNKYFGPFASSKFRLRDIYDLLLKLFPLRKCAPHERGHPCFYYQLKMCMGQCMGEDTPERYQTTVKGIEQFFNHGPEQVLNHLQQQEIKASEQQNFEAARHFLDLQKAVLELVNMQQTAFIKAKQSHDFIGYVFEKNVLAITVFAYVDNQLIGKNQQVFELPQDDEKEVESALVTFIYHYYSTNKIPKTLTVSLSEENLSLLANSLKINVTQPKNGEQKSILQTVIDNARYALNTKWTGFINNLNRAEVHQQLAQLLQVPSIQSLEIIDISFYDKDHVVGAMLRYENGKRMKALSRRYNINIDHKGDTNYMADVVYRRIISSIQTHKQLPLSDLLIVDGGIAQINTVTKVFASFPNVTQPIIIGLAKNTRHQTDHIVLTDNTTINIDKNTPLFAYLTTIQEEVDSFAKHNAFKRVSRARFQNPLLQIEGVGRKTVQILLDNFQTMQNIEAASLSELSQFIPANLAQKIKTFLKEND" }, "paralog": false, "identity": 0.9934924078091106, "score": 871.0, "evalue": 6.05e-321, "description": "Internal stop codon at 161768, 162779. Nonsense mutation around 161768, 162779.", "psc": { "uniref90_id": "UniRef90_P75350", "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_P47448", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75350", "UniRef:UniRef50_P47448", "KEGG:K03703", "GO:0003677", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P47448", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47448" ], "product": "UvrABC system protein C" } }, "id": "GIHJHMOALN_319", "locus": "GCA009939745_00284" }, { "type": "cds", "sequence": "contig_1", "start": 162815, "stop": 163501, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14520.12", "PFAM:PF08459.17" ], "nt": "GTGCACCAGCAATTAGCGCAGCTTTTACAAGTTCCTTCGATTCAAAGTCTGGAAATTATTGACATCTCCTTTTATGACAAAGACCACGTGGTGGGGGCGATGTTACGGTACGAAAATGGCAAACGGATGAAAGCGTTATCACGCCGTTACAACATTAACATTGACCATAAGGGTGACACTAACTATATGGCTGATGTGGTGTACCGCCGGATTATTTCGTCCATCCAAACCCATAAACAGCTACCTTTAAGTGATCTTTTAATTGTCGATGGTGGGATTGCCCAAATTAATACGGTAACCAAGGTGTTTGCCTCTTTCCCTAACGTTACCCAACCGATCATTATTGGCTTAGCGAAAAACACCCGTCACCAAACTGACCACATTGTGTTAACGGATAACACTACCATTAACATTGACAAAAATACGCCTTTGTTTGCTTATCTTACCACCATTCAAGAAGAGGTCGATAGCTTTGCCAAACACAATGCCTTTAAGCGAGTTAGTCGCGCTCGCTTCCAAAATCCACTCTTACAAATTGAAGGGGTTGGGCGAAAAACTGTCCAAATATTACTTGATAATTTTCAAACAATGCAAAACATTGAAGCTGCTTCTTTGAGCGAATTATCACAGTTTATTCCTGCTAATTTAGCACAAAAAATTAAAACTTTTCTAAAGGAAAATGACTAA", "aa": "MHQQLAQLLQVPSIQSLEIIDISFYDKDHVVGAMLRYENGKRMKALSRRYNINIDHKGDTNYMADVVYRRIISSIQTHKQLPLSDLLIVDGGIAQINTVTKVFASFPNVTQPIIIGLAKNTRHQTDHIVLTDNTTINIDKNTPLFAYLTTIQEEVDSFAKHNAFKRVSRARFQNPLLQIEGVGRKTVQILLDNFQTMQNIEAASLSELSQFIPANLAQKIKTFLKEND", "aa_hexdigest": "89a07411c6a504027f0cf30b313274b6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14520.12", "name": "HHH_5", "length": 54, "aa_cov": 0.2236842105263158, "hmm_cov": 1.0, "evalue": 3.722611915036173e-08, "score": 29.2736759185791, "start": 176, "stop": 226 }, { "id": "PF08459.17", "name": "UvrC_RNaseH_dom", "length": 161, "aa_cov": 0.6710526315789473, "hmm_cov": 0.9751552795031055, "evalue": 5.201793044828769e-34, "score": 112.88227081298828, "start": 7, "stop": 159 } ], "seq_stats": { "molecular_weight": 25918.44150000002, "isoelectric_point": 9.046477699279784 }, "id": "GIHJHMOALN_320", "locus": "GCA009939745_00285" }, { "type": "cds", "sequence": "contig_1", "start": 163554, "stop": 163973, "strand": "+", "frame": 3, "gene": "yfcE", "product": "putative metallophosphoesterase MPN_126", "db_xrefs": [ "COG:COG0622", "COG:R", "EC:3.1.4.-", "GO:0016787", "GO:0046872", "KEGG:K07095", "SO:0001217", "UniRef:UniRef50_P47449", "UniRef:UniRef90_P75349" ], "nt": "ATGAAGTTGCACAACCCTGATGTAGTAATTCATGCTGGTGATCATTTAACTACTAAAAAGTTTATGGATCAAAATGCCACTTTTTGGGTTGCTGGTAATCATGATGTTGTTGGCGAAGAGATTCAAATGTTTGAGTTAGAGGGAATCCAGTTTGTTTTAATGCACGGCCATCAAGCGCCCCGTCATGATCTCAAGCAGTGGTATAAAATGCTGGTTGATCAAGCTAAAAGCTATCTTTGTGATGTTTTAATTGTGGGTCACAGTCACATAGAGCACTATGAAACTATTGATGGGATCCAGGTTATTAACCCCGGTTCTCTAGAAATTCCCCGTAATCCGAGAAAGCTACCTACCTACTGTAATTTAAACTTAAGTCAGGGCAGGATAAGTGATTTAACGTTCCATTTTCCTCGGGATTAA", "aa": "MKLHNPDVVIHAGDHLTTKKFMDQNATFWVAGNHDVVGEEIQMFELEGIQFVLMHGHQAPRHDLKQWYKMLVDQAKSYLCDVLIVGHSHIEHYETIDGIQVINPGSLEIPRNPRKLPTYCNLNLSQGRISDLTFHFPRD", "aa_hexdigest": "a7871139f2662e9bb2626c69be59a706", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75349", "query_cov": 1.0, "subject_cov": 0.8742138364779874, "identity": 1.0, "score": 297.0, "evalue": 5.05e-102, "valid": true, "gene": "YfcE", "product": "Probable metallophosphoesterase MPN_126", "ec_ids": [ "3.1.4.-" ], "uniref50_id": "UniRef50_P47449", "cog_id": "COG0622", "cog_category": "R", "kegg_orthology_id": "K07095", "go_ids": [ "GO:0016787", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47449", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47449" ], "product": "Probable metallophosphoesterase MG207" }, "genes": [ "yfcE" ], "id": "GIHJHMOALN_321", "locus": "GCA009939745_00286" }, { "type": "cds", "sequence": "contig_1", "start": 164422, "stop": 165180, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22", "PFAM:PF23991.1", "PFAM:PF04102.17" ], "nt": "GTGACGAACCACCTTCTTAAACATTTTTACGTTTTTCCAATCAATAATAAAGGAGGTCTTGCCATTAAACGCATAATTTCACTTGCTTTGTTTAAGAAAAGACTTAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGGATTTAATGGCAATTTCAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAACAATCAGCGTCTCATAAAGATTGAAACTACTTTGGCTATCCAAGGCGAACAAATCAACAAATTGACTCAAACTGTTGAAAAGCAAGGCGAACAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCAGGGCGAGCAAATTAGAGAACTTCAAGTGGAGCAAAAAGCACAAAGACAAGAGTTTAATGCCCGCATGGATCGTTTGGAAAATCTTTTGGTGGAAAGTATAGAATCTACCAATAATCGCTTCGACTCTATGGAAAGACGTTTAGACTCTATGGATAGTCGTCTTGATTCTATGGAAAATCGCTTGGTTTCAATGGAAAGCCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTTGTCTTGATTTTGTTGAAGGACGCTTAGACTCTATGGAAACTCGTTTAGACTCTATGGAAACTCGCCTGGACAAAGTCGATCCGCCCAAATAG", "aa": "MTNHLLKHFYVFPINNKGGLAIKRIISLALFKKRLNKDKINNCHVWEEELPDGSYDMGFNGNFNHMEKRKSGYVTQKQFSEFKDANNQRLIKIETTLAIQGEQINKLTQTVEKQGEQINQLVQVVLLQGEQIRELQVEQKAQRQEFNARMDRLENLLVESIESTNNRFDSMERRLDSMDSRLDSMENRLVSMESRLDSMENRLDSMEGRLDSMENRLDSMEGCLDFVEGRLDSMETRLDSMETRLDKVDPPK", "aa_hexdigest": "a30c580dfad6aaa4d466e23cd3b21ad9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 68, "aa_cov": 0.25793650793650796, "hmm_cov": 1.0, "evalue": 2.265764889048572e-39, "score": 129.62060546875, "start": 59, "stop": 123 }, { "id": "PF23991.1", "name": "DUF7310", "length": 57, "aa_cov": 0.2261904761904762, "hmm_cov": 1.0, "evalue": 3.4423436846481874e-14, "score": 48.62373352050781, "start": 190, "stop": 246 }, { "id": "PF04102.17", "name": "SlyX", "length": 58, "aa_cov": 0.23015873015873015, "hmm_cov": 1.0, "evalue": 1.4450551950796683e-08, "score": 30.75282096862793, "start": 87, "stop": 144 } ], "seq_stats": { "molecular_weight": 29513.11560000001, "isoelectric_point": 5.3499361038208 }, "id": "GIHJHMOALN_322", "locus": "GCA009939745_00287" }, { "type": "cds", "sequence": "contig_1", "start": 165437, "stop": 165718, "strand": "+", "frame": 2, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "RefSeq:WP_015344878.1", "SO:0001217", "UniParc:UPI0002B84587", "UniRef:UniRef100_A0AAP8E4D3", "UniRef:UniRef50_A0AAP8E4D3", "UniRef:UniRef90_A0AAP8E4D3" ], "nt": "ATGAGTAATACAAAAAAATTAAGAATAACTTCAATCGAAGAAACAATAGAAATAATAGTTAATTCAAAACTATTAGCTTTTGGTTTTTACCCAGTTGGTTTAGAAGATGGAACTTACGAACTTAATGTAAGGATTATTTTTAAAGAAAAAACTGCAGATTCTGAGAAGAGTAGCAGTGTACATCCTTTACAAAAGGAGTATTATGTAAGGGAATTTTTGAGATTTCTTGAGCGCCATAAAGATTCTCTGGCGCAACAACTATTAGTAGTAGAAGACAATTAA", "aa": "MSNTKKLRITSIEETIEIIVNSKLLAFGFYPVGLEDGTYELNVRIIFKEKTADSEKSSSVHPLQKEYYVREFLRFLERHKDSLAQQLLVVEDN", "aa_hexdigest": "6535a3d15727147d2970eafc4bef2012", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B84587", "ncbi_nrp_id": "WP_015344878.1", "uniref100_id": "UniRef100_A0AAP8E4D3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B84587", "RefSeq:WP_015344878.1", "UniRef:UniRef100_A0AAP8E4D3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E4D3", "uniref90_id": "UniRef90_A0AAP8E4D3" }, "psc": { "uniref90_id": "UniRef90_A0AAP8E4D3", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D3" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D3" ], "product": "Family K-like protein" }, "genes": [], "id": "GIHJHMOALN_323", "locus": "GCA009939745_00288" }, { "type": "cds", "sequence": "contig_1", "start": 165971, "stop": 166228, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGAACCTGCGCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNSAKGITAGSGSQQTTYEPARTEAALTASTTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "54aa34d2360fc2a5f30de0b05c27255a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 2.6707607909721974e-09, "score": 32.272003173828125, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9074.855599999997, "isoelectric_point": 8.138311576843265 }, "id": "GIHJHMOALN_324", "locus": "GCA009939745_00289" }, { "type": "cds", "sequence": "contig_1", "start": 166259, "stop": 166852, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_128", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75347", "UniRef:UniRef90_P75347" ], "nt": "GTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGAGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATCCAGCAAGTCCAACTCAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGAAGGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCCTTTAA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRELGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNPASPTQGSRLRHHPIQFGIWSIRHPHPLKAVACDRANSQGPPQMIRLDPHSVRCALCL", "aa_hexdigest": "685101d07832070a93dbdfcf293db11c", "start_type": "GTG", "rbs_motif": "AATAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 165959, "stop": 166852, "observations": { "mutation": [ 166226 ], "stop": [ 166226 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75347", "query_cov": 1.0, "subject_cov": 0.5225464190981433, "identity": 0.995, "score": 379.0, "evalue": 2.56e-130, "gene_start": 1, "gene_end": 197, "reference_start": 181, "reference_end": 377, "reference_sequence": "MISPKLDWLILTATASLAAGLTVVGHFTSTTTTLKRQQFSYTRPDEVALRHTNAINPRLTPWTYRNTSFSSLPLTGENPGAWALVRDNSAKGITAGSGSQQTTYEPARTEAALTASTTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKNKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRELGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNPASPTQGSRLRHHPIQFGIWSIRHPHPLKAVACDRANSQGPPQMIRLDPHSVRCALCL" }, "paralog": false, "identity": 0.9966329966329966, "score": 592.0, "evalue": 2.79e-216, "description": "Internal stop codon at 166226. Nonsense mutation around 166226.", "psc": { "uniref90_id": "UniRef90_P75347", "product": "Uncharacterized protein MPN_128", "uniref50_id": "UniRef50_P75347", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75347", "UniRef:UniRef50_P75347" ] }, "pscc": { "uniref50_id": "UniRef50_P75347", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75347" ], "product": "Uncharacterized protein MPN_128" } }, "id": "GIHJHMOALN_325", "locus": "GCA009939745_00290" }, { "type": "cds", "sequence": "contig_1", "start": 167552, "stop": 167908, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_129", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75346", "UniRef:UniRef90_P75346" ], "nt": "ATGGTCTCTTTTTTTGATATATATACCAAAGCATCGATTGTAGAAGCTGTACGTTTTTCTTTTTTGAATACCTTTCAATTAAAGGAACTTGGTTTACCGATTAAAGAAATTGCTGTACCATTAGGCACATTAGTCTTTCTTTTTGTTGTTATTATTACCTTAATTCCGTTGTTAATTATTGGTAATTTAATTTGGACTAACTTGCGTTTAATAGAACGGGAAAATACACAGCAGTACCAACTCGTTTTTGGTTACAGTTTGATAGTTAGTGATATTGTTGGTTTTGCTATTGTTTTCTTCGGTGCCATTTTAGGTACAAATTTAAAAAGTGTTGAATTATTCATAGCATTGGGTTGA", "aa": "MVSFFDIYTKASIVEAVRFSFLNTFQLKELGLPIKEIAVPLGTLVFLFVVIITLIPLLIIGNLIWTNLRLIERENTQQYQLVFGYSLIVSDIVGFAIVFFGAILGTNLKSVELFIALG", "aa_hexdigest": "cd2e740ca967c78b750e6197fc5c17b1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 167552, "stop": 168001, "observations": { "mutation": [ 167906 ], "stop": [ 167906 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75346", "query_cov": 1.0, "subject_cov": 0.7919463087248322, "identity": 1.0, "score": 214.0, "evalue": 9.18e-70, "gene_start": 1, "gene_end": 118, "reference_start": 1, "reference_end": 118, "reference_sequence": "MVSFFDIYTKASIVEAVRFSFLNTFQLKELGLPIKEIAVPLGTLVFLFVVIITLIPLLIIGNLIWTNLRLIERENTQQYQLVFGYSLIVSDIVGFAIVFFGAILGTNLKSVELFIALGWMMMLGSLIALGTTANLVSSIYLYIKLALKR" }, "paralog": false, "identity": 0.9932885906040269, "score": 278.0, "evalue": 1.79e-97, "description": "Internal stop codon at 167906. Nonsense mutation around 167906.", "psc": { "uniref90_id": "UniRef90_P75346", "product": "Uncharacterized protein MPN_129", "uniref50_id": "UniRef50_P75346", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75346", "UniRef:UniRef50_P75346", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75346", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75346" ], "product": "Uncharacterized protein MPN_129" } }, "id": "GIHJHMOALN_326", "locus": "GCA009939745_00291" }, { "type": "cds", "sequence": "contig_1", "start": 167984, "stop": 168391, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ], "nt": "GTGGTTTTCGTCACTTTGTCCGTTCTTATCATTCCTTCACCCCGCGCCGGTTCAGAGCAGGAGGTTGGCTTGGTCGAGGGTGGGTCATTTGGAGCCGCCTGTAGTCGTCTTCTGACTGCCACCAAGCGTGGCTTGGACGTTGGCTTTGAGGCCAACGCCGACATTCGAGAACGTAGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTTAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTCCCATACCTGTACCCATTCCCAATCCAGGGCACATAATCCAACACCTAGTCGAGAACCGACCCCGCGAGGTTGAACCCGGTGGAGAAGCGCGTGAGCGTGGGGGAGAAGTGTGTCTATCTCTTCAATGCTAG", "aa": "MVFVTLSVLIIPSPRAGSEQEVGLVEGGSFGAACSRLLTATKRGLDVGFEANADIRERREKGTRTTCVRTSSAGRPRAGFRRGYIIHAPVVIPIPVPIPNPGHIIQHLVENRPREVEPGGEARERGGEVCLSLQC", "aa_hexdigest": "67335385041c51049ca065e41dce58c8", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "query_cov": 0.9851851851851852, "subject_cov": 0.9432624113475178, "identity": 0.812, "score": 203.0, "evalue": 2.6e-65, "valid": false, "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14521.533299999986, "isoelectric_point": 9.238915824890135 }, "id": "GIHJHMOALN_327", "locus": "GCA009939745_00292" }, { "type": "cds", "sequence": "contig_1", "start": 168970, "stop": 169167, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0AAN4XEA6" ], "nt": "ATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTTTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGCTGACACTGAGACGACAAATAAGGAAAAGCCTTTAGGCACTTGA", "aa": "MKKVKALRNLFLEKASEKLEYPKIASMVAYFDPKEDGTCEFIMKIKTVNEVADTETTNKEKPLGT", "aa_hexdigest": "8f5463db56fabcd688e705e868eeecf6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XEA6", "query_cov": 1.0, "subject_cov": 0.8552631578947368, "identity": 0.985, "score": 124.0, "evalue": 3.56e-36, "valid": true, "uniref50_id": "UniRef50_A0A0H3DJX7" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7412.577499999999, "isoelectric_point": 7.800045967102052 }, "id": "GIHJHMOALN_328", "locus": "GCA009939745_00293" }, { "type": "cds", "sequence": "contig_1", "start": 169308, "stop": 169565, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D96482" ], "nt": "GTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAATGTCAACGGCACCTTAAGTGGTTCGCGAACTACGCCTAATCAGCAAGGCACTCCCTGGTTAACCCTCGACCAAGCCAACCTCCAGCTCTGA", "aa": "MLDYVPWIGNGYRYGNNHRGSNSSTSGVTTQGQSQNASSNEPAPTFSNVGVGLKANVNGTLSGSRTTPNQQGTPWLTLDQANLQL", "aa_hexdigest": "f682510355e5f0014a5647b598ae3463", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002D96482", "query_cov": 1.0705882352941176, "subject_cov": 0.8504672897196262, "identity": 0.593, "score": 95.9, "evalue": 5.27e-24, "valid": false, "uniref50_id": "UniRef50_UPI0002D96482" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D96482", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D96482" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8982.590799999998, "isoelectric_point": 8.209807014465333 }, "id": "GIHJHMOALN_329", "locus": "GCA009939745_00294" }, { "type": "cds", "sequence": "contig_1", "start": 169856, "stop": 170179, "strand": "+", "frame": 2, "gene": null, "product": "Adhesin P1-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6", "UniRef:UniRef90_A0AB33HTF6" ], "nt": "GTGCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTCAATAAGAGTGGGCAAGATGATAACAGTAAGTTTAATTCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCTTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCAGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MQLFLRGLLGSIPVLVNKSGQDDNSKFNSTDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTLTSSTANQNSTTVPSIGFKIPEQNNDSKAVLITPGLA", "aa_hexdigest": "bec1a9e68a7a9049e29073db24c1c2a0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 169823, "stop": 170482, "observations": { "mutation": [ 170177, 170321 ], "stop": [ 170177, 170321 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HTF6", "query_cov": 1.0, "subject_cov": 0.4863636363636364, "identity": 0.991, "score": 207.0, "evalue": 3.23e-66, "gene_start": 1, "gene_end": 107, "reference_start": 12, "reference_end": 118, "reference_sequence": "MSFTNKNNAQRVQLFLRGLLGSIPVLVNKSGQDDNSKFNSTDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTLTSSTANQNSTTVPSIGFKIPEQNNDSKAVLITPGLAWTPQDVGNLVVSGTSLSFQLGGWLVTVHGLYQTPRGLFGPPVKWPGCQWQRPKGVNLGQAALSGISWQLGQPVGTGRECVGFEGGVGGSSSQRGVRVASSYK" }, "paralog": false, "identity": 0.9863013698630136, "score": 437.0, "evalue": 3.09e-159, "description": "Internal stop codon at 170177, 170321. Nonsense mutation around 170177, 170321.", "psc": { "uniref90_id": "UniRef90_A0AB33HTF6", "product": "Adhesin P1 homolog", "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HTF6", "UniRef:UniRef50_A0AB33HTF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HTF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HTF6" ], "product": "Adhesin P1 homolog" } }, "id": "GIHJHMOALN_330", "locus": "GCA009939745_00295" }, { "type": "cds", "sequence": "contig_1", "start": 170394, "stop": 171041, "strand": "+", "frame": 3, "gene": null, "product": "Putative adhesin P1-like protein MPN_132", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75266", "UniRef:UniRef90_P75266" ], "nt": "TTGGGACGGGTCGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGACACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTCCACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGCTCCGGTCAAACCAATTCCCAAAACACCTCCCCCTACCTGCATTTGATTAAACCTAAAAAAGTTACTGCTTCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGGGTGAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAACCCCAACCCCAAGCCCTCAAAACAACGACACCGGTGTTTGGTACGAATAGTGGTAACCTCGGTAGTGTGCTTAGTGATGGGGGTACCACGCAGGACTCAAGCACCACCAATCAACTGTCACCCGTTGAACGGGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGAGGTGGTGGGGGTGGGAGATTTGTCTAAGCGAGCTTCCATTGAATCAAGTCGTTTGTGA", "aa": "MGRVESVWDLKGVWADQAHSAVSESQAATSSTTTTATGDTLPEHPNALAYQISSTDKDSYKASTQGSGQTNSQNTSPYLHLIKPKKVTASDKLDDDLKNLLDPNEVRVKLRQSFGTDHSTQPQPQALKTTTPVFGTNSGNLGSVLSDGGTTQDSSTTNQLSPVERVSGWLVGQLPSTSDGNTSSTNNLAPNTNTGNEVVGVGDLSKRASIESSRL", "aa_hexdigest": "e0704ab5106e9253f09f5194f21a5020", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75266", "query_cov": 1.0093023255813953, "subject_cov": 0.8410852713178295, "identity": 0.977, "score": 394.0, "evalue": 9.93e-138, "valid": true, "product": "Putative adhesin P1-like protein MPN_132", "uniref50_id": "UniRef50_P75266" }, "pscc": { "uniref50_id": "UniRef50_P75266", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75266" ], "product": "Putative adhesin P1-like protein MPN_132" }, "genes": [], "id": "GIHJHMOALN_331", "locus": "GCA009939745_00296" }, { "type": "cds", "sequence": "contig_1", "start": 171038, "stop": 171205, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATAGCATTAAAACCTTAGCTAACGCTAACTTTGGGCGATTTAATCCACTTAGTTTTGAACTCCTTAAATTTGTGTTTTTCTTGACTTTGCTAAAAAAACAATTCAAAGAGGCTTGCAATGAACAGGTCAGCACTGCTAAATTAGTGAGTGCAAGAACTAAATAA", "aa": "MNSIKTLANANFGRFNPLSFELLKFVFFLTLLKKQFKEACNEQVSTAKLVSARTK", "aa_hexdigest": "15f3853397ef10fa7c81b8e6b4e63f76", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6284.353900000002, "isoelectric_point": 10.135606575012208 }, "id": "GIHJHMOALN_332", "locus": "GCA009939745_00297" }, { "type": "cds", "sequence": "contig_1", "start": 171517, "stop": 171993, "strand": "+", "frame": 1, "gene": "yncB", "product": "putative lipo MG186-like protein", "db_xrefs": [ "COG:COG1525", "COG:L", "GO:0004519", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75265", "UniRef:UniRef90_P75265" ], "nt": "ATGAAAGGGTTTTCTTGCTCCAGACCGGGCTATCTTACTGGTCTCTTGCTCCTCGCTGTAGCCCCTATTCTCACTGCTTGTACCCGCGACTATACTACCAAGAACGAATTTCAATTAACAACAGCTCAACAGGCCAAATTAAAGCCAGCCACCATCGAATATTGGCGTGATGGTGATACGCCTGAGATCAATTACGCAAGCGAAGAGCGCCGAAAGGAAGCTGAACAAAAATCTAAAGAAAACGCTAAGAAAGAAGACAAAAAAGAGGAAAAGAAAACTGAAGATTCCCAAGATTCTTCTTCTGCATCCACGCAAGTTCGTAGTAGTAAACACGGTCTCCGCATCTATGGAATAGATACACCCGAAAAACATGTAAGTAGCAAAGGCGATAGCACCGGTGATGAAAAAATCGAAGCCGAAAAGGCCTCTAACTATGCTGAAAAGCTAATCCCTAAAGGCAGTACTGTTTGGGTTTGA", "aa": "MKGFSCSRPGYLTGLLLLAVAPILTACTRDYTTKNEFQLTTAQQAKLKPATIEYWRDGDTPEINYASEERRKEAEQKSKENAKKEDKKEEKKTEDSQDSSSASTQVRSSKHGLRIYGIDTPEKHVSSKGDSTGDEKIEAEKASNYAEKLIPKGSTVWV", "aa_hexdigest": "8111908103a440578a0df78a47b4c3c9", "start_type": "ATG", "rbs_motif": null, "genes": [ "yncB" ], "truncated": "3-prime", "pseudogene": { "start": 171517, "stop": 172296, "observations": { "mutation": [ 171991 ], "stop": [ 171991 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75265", "query_cov": 1.0, "subject_cov": 0.5249169435215947, "identity": 1.0, "score": 257.0, "evalue": 6.1e-84, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MKGFSCSRPGYLTGLLLLAVAPILTACTRDYTTKNEFQLTTAQQAKLKPATIEYWRDGDTPEINYASEERRKEAEQKSKENAKKEDKKEEKKTEDSQDSSSASTQVRSSKHGLRIYGIDTPEKHVSSKGDSTGDEKIEAEKASNYAEKLIPKGSTVWVWSLNTYSYDREVGALFFKSNPKQTFFQSFEVAMVEAGHAIPIAGTGLNLIADPELSADDPLSVIGLQLANAANKAYNAKINIWSHDTDGYRSLTAVYKLRGADISWTRFLDEANGYSSASAGTGASLYQLWDQRQAKLAQKGS" }, "paralog": false, "identity": 0.9961389961389961, "score": 458.0, "evalue": 1.75e-165, "description": "Internal stop codon at 171991. Nonsense mutation around 171991.", "psc": { "uniref90_id": "UniRef90_P75265", "gene": "YncB", "product": "Uncharacterized lipoprotein MG186 homolog", "uniref50_id": "UniRef50_P75265", "cog_id": "COG1525", "cog_category": "L", "go_ids": [ "GO:0005886", "GO:0004519" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75265", "UniRef:UniRef50_P75265", "COG:COG1525", "COG:L", "GO:0005886", "GO:0004519" ] }, "pscc": { "uniref50_id": "UniRef50_P75265", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75265" ], "product": "Uncharacterized lipoprotein MG186 homolog" } }, "id": "GIHJHMOALN_333", "locus": "GCA009939745_00298" }, { "type": "cds", "sequence": "contig_1", "start": 172024, "stop": 172308, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCGCTTTATTCTTTAAGTCTAACCCTAAGCAAACCTTCTTCCAATCCTTTGAAGTAGCTATGGTTGAGGCTGGTCATGCTATTCCAATTGCTGGGACTGGTTTAAATTTAATCGCTGATCCGGAATTAAGTGCTGATGATCCTTTAAGTGTCATTGGTCTACAGTTAGCCAACGCTGCCAATAAGGCTTACAACGCTAAAATTAACATTTGGAGCCATGACACTGATGGTTATCGTTCCTTGACTGCTGTTTACAAGTTAAGGGGCGCTGATATTAGTTGA", "aa": "MGALFFKSNPKQTFFQSFEVAMVEAGHAIPIAGTGLNLIADPELSADDPLSVIGLQLANAANKAYNAKINIWSHDTDGYRSLTAVYKLRGADIS", "aa_hexdigest": "c9560ff980c34fa65a91b6462f8af8b7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10070.29969999999, "isoelectric_point": 5.466001319885254 }, "id": "GIHJHMOALN_334", "locus": "GCA009939745_00299" }, { "type": "cds", "sequence": "contig_1", "start": 172446, "stop": 172790, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU7Q6T1" ], "nt": "ATGGAAAAGATACAAGCGGAAAAATCCCAAAGTGCAATTGAGTTTAAGAATATTGTAGTCGACTTTGGTGAGTCAATCGCAATTGATAACATTAACCTCACGGTTAAAAAGAAGGAGTTAGTTACCTTACTAGGTCCTTCTGGGTGTGGGAAAACAACTTCGCTTTCCGTGATTGCGGGTCTTATCGCCCCTACTAGTGGTCAGGTTTTATTTAATGGTTATGATGTCACAAAAAAGCCACCACAACAACGCAAACTGGGATTAGTGTTCCAAAACTATGCCTTGTATCCGCACATGAGTGTGTTTGAAAACATTGTCTTCCCACTCTACAGTGATACGTCTTGA", "aa": "MEKIQAEKSQSAIEFKNIVVDFGESIAIDNINLTVKKKELVTLLGPSGCGKTTSLSVIAGLIAPTSGQVLFNGYDVTKKPPQQRKLGLVFQNYALYPHMSVFENIVFPLYSDTS", "aa_hexdigest": "875cdab9b3219eea9ca66ce6958763fc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAU7Q6T1", "query_cov": 0.8859649122807017, "subject_cov": 0.8938053097345132, "identity": 0.505, "score": 116.0, "evalue": 1.69e-31, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0AAU7Q6T1" }, "pscc": { "uniref50_id": "UniRef50_A0AAU7Q6T1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU7Q6T1" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "GIHJHMOALN_335", "locus": "GCA009939745_00300" }, { "type": "cds", "sequence": "contig_1", "start": 172842, "stop": 173210, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCTTAAAGGCTAATGGTGCTACTAGTGAGGAGCTGGCAGAGCTCAATCGTTTAATGCAACAACGAATCGATGAGCCCAAACGAATGGCTTACCAGATTAATGATTTGATGGTAAGTGTGTTTCAAAAACAGAGTGAACTGGAAGCTAACCTAAAGTTAATTCCCCGCAAAAAACAGTTTGCCATTATTTCACTCAGCAAGGAAACACTAAGTCAAATAAGGGATGTTGAAACTAAAGCCAAGGCTGCATTGGAAACCGCTGATAGTGCTGAAGTGGAGCAGACCATTAAGAGTGAACTTAAGCAAAAACTAAGTGAAATTAAGGCTAATTACCACGATGAAAAGGCCAACATTAAAGCTTACTGA", "aa": "MILKANGATSEELAELNRLMQQRIDEPKRMAYQINDLMVSVFQKQSELEANLKLIPRKKQFAIISLSKETLSQIRDVETKAKAALETADSAEVEQTIKSELKQKLSEIKANYHDEKANIKAY", "aa_hexdigest": "8e8b9dee8047eaeb172646f3bb40250d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13922.866499999982, "isoelectric_point": 7.878310585021973 }, "id": "GIHJHMOALN_336", "locus": "GCA009939745_00301" }, { "type": "cds", "sequence": "contig_1", "start": 173217, "stop": 173306, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGCCAATATTAAGACCGAATTAAAAACCGAAAAAACTGCCATTAAGCAAACTAATGACTATGCCAAATTGAAGGAATTGAAGTGA", "aa": "MLANIKTELKTEKTAIKQTNDYAKLKELK", "aa_hexdigest": "e1eaa73ae50a081dc00917ea6413f12d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3364.949300000001, "isoelectric_point": 9.523737144470214 }, "id": "GIHJHMOALN_337", "locus": "GCA009939745_00302" }, { "type": "cds", "sequence": "contig_1", "start": 173325, "stop": 173894, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGAATTTAAAGAAGCAGTACCGCAGTTACTTTAAACAACTAAAAGCCAAATACAGCTTAAAAGATGGCAATTTAACTGAAAGCGAATTAAGTCAAATTGAGGAGTTGCAAAAACGGATTGTTTCGCTCAAAGATTTCATTAACCGCACTGCTAAAGAAGTAGCTGAAAAACTGGAAATTACTAAGATTCTCCACAAGCGCCCCGCTAATATTTCTGGGGGTCAACAACAACGGGTAGCTATTGCTAGAGCAATTGTTAGAAGACCTAAGGTTCTACTTATGGACGAACCGTTGTCCAACTTAGATGCGAAGTTGCGGGTACAAACACGGCAATGGATCCGTAAGTTCCAGCAAGACCTGCAAATTACTACTGTCTTTGTGACTCACGATCAGGAAGAGGCAATGAGTATTAGCGACACGATTGTCTGCATGTCAACTGGTAAGGTACAGCAAATAGGCTCCCCTTCCGAGTTGTACTTAAAACCGGCCAATGAGTTCGTTGCTACCTTTTTGGGTAGCCCGGAAATGAATATCGTCAACGCTACGGTTAAAGCTGGACAGCTGTTGTGA", "aa": "MNLKKQYRSYFKQLKAKYSLKDGNLTESELSQIEELQKRIVSLKDFINRTAKEVAEKLEITKILHKRPANISGGQQQRVAIARAIVRRPKVLLMDEPLSNLDAKLRVQTRQWIRKFQQDLQITTVFVTHDQEEAMSISDTIVCMSTGKVQQIGSPSELYLKPANEFVATFLGSPEMNIVNATVKAGQLL", "aa_hexdigest": "fc9d91ecccb12c6f47af9d5cf661e7e6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 57, "aa_cov": 0.2804232804232804, "hmm_cov": 1.0, "evalue": 1.2433115915860745e-14, "score": 50.5290412902832, "start": 46, "stop": 98 } ], "seq_stats": { "molecular_weight": 21531.76039999999, "isoelectric_point": 9.680137443542481 }, "id": "GIHJHMOALN_338", "locus": "GCA009939745_00303" }, { "type": "cds", "sequence": "contig_1", "start": 174024, "stop": 174206, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAACCTAGGGAAACACATGGTTGGGGTTGTGGAGAGCAATGGGGTACAGCTCAATGTGCGCTTAGAATTGAGCCACCAGTTTGAAGTAGGTAATGCAGTTAAGTTTACGATTAAGCCTAATGGCTTACACTTCTTTGATCCCCAAACAACGCAACGGGTGGAGGTAAAACATGTTTAA", "aa": "MENLGKHMVGVVESNGVQLNVRLELSHQFEVGNAVKFTIKPNGLHFFDPQTTQRVEVKHV", "aa_hexdigest": "d0b0d10ab17a162e6af31251d2a1c603", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6784.6942, "isoelectric_point": 6.901981544494629 }, "id": "GIHJHMOALN_339", "locus": "GCA009939745_00304" }, { "type": "cds", "sequence": "contig_1", "start": 174289, "stop": 174573, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTGTTTTGCCCAGCTTTATTAACTACCTTTCTCTTTACTTTAGTACCGTTCTTCCTTACGTTACAGAAAGGGTTTAGTCACAATGAGGACATCTACCGGGTTGATTCCCAACAGTTTGGCTTTCAAACTTTCGCTAACCTTTTTAGTGAGTCCAACTTTATTTTGGGCTTGCGCAATTCCTTTTTGTACTCGATTATTTCCCTACCACTGACAATTGTGCTAGCAATTATTATTTCTAGTGCCATTGTCTTTGTGTACCGGAAGTTAGCACGGGGCTTTTGA", "aa": "MLFCPALLTTFLFTLVPFFLTLQKGFSHNEDIYRVDSQQFGFQTFANLFSESNFILGLRNSFLYSIISLPLTIVLAIIISSAIVFVYRKLARGF", "aa_hexdigest": "c7d05f9b2e85e6f87ed8946a8956561a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10754.560499999989, "isoelectric_point": 9.032939338684084 }, "id": "GIHJHMOALN_340", "locus": "GCA009939745_00305" }, { "type": "cds", "sequence": "contig_1", "start": 174640, "stop": 175134, "strand": "+", "frame": 1, "gene": "ugpA", "product": "putative ABC transporter permease MG188-like protein", "db_xrefs": [ "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75263", "UniRef:UniRef90_P75263" ], "nt": "TTGGACTCCTCTTCGGGAATCTTAAACAACATCTTTCATGTCAACATTAAGTGGTTAGATTCGGGGGAACGTGATACCTTTAATGCCTTATGGGGAATTCTTATCTTTGGAATTTGGAAGAACATGGCTTTTAATGTGCTGGTAATATCAACCGCCATGTTAAGTGTGGATCCTACTTTATATAAGGTAGCTAACTTAGATGGTGCAAAACCAATTAGGCAGTTCTTTAAGATTACCTTACCTTCAATCCGCCCAACCTTAATTTTCCTGTTGACCCTGTTAATTTTGGGCGGAATGCAGGTGTTTCCAATTTCGTTGTTTAACGGGAATGATAGTGAAGCTGTAACCAATGGGGGTTCGACCATCTTGCTTTACATCTTCCAAAAGATTCGTGATCAGAATAACAATTTTGCTGGTGCTGCTACCTTGGTGTTGTTCATCTTGGGGGTTTGTTATGGGTTAGTGTTGCGCAATGGTTTTCGCTTAATTGAATGA", "aa": "MDSSSGILNNIFHVNIKWLDSGERDTFNALWGILIFGIWKNMAFNVLVISTAMLSVDPTLYKVANLDGAKPIRQFFKITLPSIRPTLIFLLTLLILGGMQVFPISLFNGNDSEAVTNGGSTILLYIFQKIRDQNNNFAGAATLVLFILGVCYGLVLRNGFRLIE", "aa_hexdigest": "d7c01eccbfee5aff83d8ab306d5babb7", "start_type": "TTG", "rbs_motif": null, "genes": [ "ugpA" ], "truncated": "both", "pseudogene": { "start": 174340, "stop": 175188, "observations": { "mutation": [ 174571, 175132, 175141 ], "stop": [ 174571, 175132, 175141 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75263", "query_cov": 1.0, "subject_cov": 0.49848024316109424, "identity": 0.9940000000000001, "score": 323.0, "evalue": 2.94e-109, "gene_start": 1, "gene_end": 164, "reference_start": 148, "reference_end": 311, "reference_sequence": "MFKWLLKHRSKPTPLELGIFDQKVSFWKPFLLFCPALLTTFLFTLVPFFLTLQKGFSHNEDIYRVDSQQFGFQTFANLFSESNFILGLRNSFLYSIISLPLTIVLAIIISSAIVFVYRKLARGFWQTVFFLPYVTSGVAVSIAFIYILDSSSGILNNIFHVNIKWLDSGERDTFNALWGILIFGIWKNMAFNVLVISTAMLSVDPTLYKVANLDGAKPIRQFFKITLPSIRPTLIFLLTLLILGGMQVFPISLFNGNDSEAVTNGGSTILLYIFQKIRDQNNNFAGAATLVLFILGVCYGLVLRNGFRLIEWAQWKIKRHYVQTKLNLV" }, "paralog": false, "identity": 0.9893617021276596, "score": 541.0, "evalue": 1.16e-197, "description": "Internal stop codon at 174571, 175132, 175141. Nonsense mutation around 174571, 175132, 175141.", "psc": { "uniref90_id": "UniRef90_P75263", "gene": "UgpA", "product": "Probable ABC transporter permease protein MG188 homolog", "uniref50_id": "UniRef50_P75263", "cog_id": "COG1175", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75263", "UniRef:UniRef50_P75263", "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75263" ], "product": "Probable ABC transporter permease protein MG188 homolog" } }, "id": "GIHJHMOALN_341", "locus": "GCA009939745_00306" }, { "type": "cds", "sequence": "contig_1", "start": 175256, "stop": 175546, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGGGTTTAATCTTTAAAACGGTGGTGTTGTGCTTCTTTGGTTTAATGGTCATCTTTCCCTTTTATTTGATGCTGGTGGTAGCACTCACCAGTGATGAAGTGGTCTTAAACATTCGTGAACCAATTCTCAAATCCGATGGGTGGCACTTCGAGAACTTCCGCCGTGTTTTGGAAGATGGTAAATACCTTAATGCCATTTGGATTAACAGCTTAGTAACAATTTTGTCGATTATCCTACGGCTTTTCTTTACCGTTAGCATGGGCTATGCCTTTTCGTTAAAAAAGTGA", "aa": "MLGLIFKTVVLCFFGLMVIFPFYLMLVVALTSDEVVLNIREPILKSDGWHFENFRRVLEDGKYLNAIWINSLVTILSIILRLFFTVSMGYAFSLKK", "aa_hexdigest": "5766d4828d1bc3b61aa50077b9d04042", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11140.34179999999, "isoelectric_point": 8.888788032531739 }, "id": "GIHJHMOALN_342", "locus": "GCA009939745_00307" }, { "type": "cds", "sequence": "contig_1", "start": 175651, "stop": 175839, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTACTACGTACCATTGGAAAGGCAATTTTGAGGAAGTACCTCAACCCTGTACAACCATCAACAAAGGCCGATTCTTTAATTCGTGGTGGAATTGCTTCAAAGGCAGTACGGAACATAAACCCAGAGAACACAGAAGCGACAAATGGCATTGTTAAGCCCAAGATAATCGCTGGTTTTTCTGGTTGA", "aa": "MVLRTIGKAILRKYLNPVQPSTKADSLIRGGIASKAVRNINPENTEATNGIVKPKIIAGFSG", "aa_hexdigest": "9574a1f301bef378bdbcaa5b0e7ef95f", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6589.668500000002, "isoelectric_point": 10.690099143981932 }, "id": "GIHJHMOALN_343", "locus": "GCA009939745_00308" }, { "type": "cds", "sequence": "contig_1", "start": 175898, "stop": 176119, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTACTAACAAACAGACCCGATCTCAACATTAACCTGTGGGTGTTGGCGCAAGGGACTGATGGCAATGCCGGGCAAAGTGACGAACAAATCCGGGTGTTGTTGAATTTAAAGATGGCAGCAGCCATTTTAGCAATTCTGCCAATGTTTATTGTTTACTTCTTGTTTAGGAAAAGGATTATGAAAGCCGTGGGATCACGTGCCAATACGATTAAAGGATAG", "aa": "MLLTNRPDLNINLWVLAQGTDGNAGQSDEQIRVLLNLKMAAAILAILPMFIVYFLFRKRIMKAVGSRANTIKG", "aa_hexdigest": "f555fe81ac1cf6d78dcb039d05c4ddb1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8129.638099999993, "isoelectric_point": 10.519902610778807 }, "id": "GIHJHMOALN_344", "locus": "GCA009939745_00309" }, { "type": "cds", "sequence": "contig_1", "start": 176697, "stop": 177104, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22", "PFAM:PF23991.1" ], "nt": "GTGAACACGGAATACGTAACGCGCAAAGAGTTTAACGAGTACAAAGACTCAAACGACCAACGACTCATAAAGATTGAAACCACTTTGGCTGCCCAAGGTGAACAAATAAGAATCCAGGGCGAGCAAATTAGAGAACTTCAAGTGGAGCAAAAAGCGCAAAGACAAGAGTTTAACGCCCGCATGGATCGTTTGGAAAACCTTTTGGTTGAAAGTATAGAATCCACCAATAAACGCTTCGACTCTATTGAAGGTCGTCTAGACTCTATGGATAGTCGTCTTGATTCTATGGAAAATCGCTTGGATTCAATAGAAGGTTGCCTTGATTCTGTTGAAGGACGTTTAGACTCTATGGAAAATCGCTTGGATTCAATGGAAACTCGCTTAGACAAAATCGATCCACCCAAATAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLIKIETTLAAQGEQIRIQGEQIRELQVEQKAQRQEFNARMDRLENLLVESIESTNKRFDSIEGRLDSMDSRLDSMENRLDSIEGCLDSVEGRLDSMENRLDSMETRLDKIDPPK", "aa_hexdigest": "ff28bdc9d3b467bde90dea85fcde23d3", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 90, "aa_cov": 0.6148148148148148, "hmm_cov": 0.9888888888888889, "evalue": 5.784052011282017e-32, "score": 105.82064819335938, "start": 2, "stop": 84 }, { "id": "PF23991.1", "name": "DUF7310", "length": 59, "aa_cov": 0.43703703703703706, "hmm_cov": 1.0, "evalue": 3.133198244895504e-12, "score": 42.34527587890625, "start": 71, "stop": 129 } ], "seq_stats": { "molecular_weight": 15860.443099999991, "isoelectric_point": 4.626091575622559 }, "id": "GIHJHMOALN_345", "locus": "GCA009939745_00310" }, { "type": "cds", "sequence": "contig_1", "start": 177509, "stop": 177889, "strand": "-", "frame": 3, "gene": null, "product": "DUF16 family-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8A6" ], "nt": "GTGGAATTCTCTGGCAGTTTCAACAGACAATTTAAACTTAACAAACCAGTTATACCAGTGAACACAGAATACGTAACGCGTAAAGAATTTAACGAGTACAAAGACTCAAACGACCAAAAACTTACAAAGATTGAAACTGAGTTAAAGAGTCAGGGTCAAAGGATTCAAGTTGTTGAGGACAAAGTTGATCGACTAACTGAAGTTGTTATGGTTCAGAGTCAACAAATCAAAACCCAGGGTGAAGCAATGAACGCCCGCATGGATCGCTTTGAAAGTCTTGTGCTTAAAAGTCTAGAATCAATCGGAAACACTTTGACTGATTTTGGTAAACGCCTCGACGTAATAGAAACTCGTTTAGACAAAATCGATCCGCCTAAATAA", "aa": "MEFSGSFNRQFKLNKPVIPVNTEYVTRKEFNEYKDSNDQKLTKIETELKSQGQRIQVVEDKVDRLTEVVMVQSQQIKTQGEAMNARMDRFESLVLKSLESIGNTLTDFGKRLDVIETRLDKIDPPK", "aa_hexdigest": "7344efc113a109584ff42b25811f186a", "start_type": "GTG", "rbs_motif": "AATAA", "psc": { "uniref90_id": "UniRef90_A0AB38W8A6", "query_cov": 0.9920634920634921, "subject_cov": 1.1261261261261262, "identity": 0.6559999999999999, "score": 140.0, "evalue": 9.4e-41, "valid": false, "product": "DUF16 family-like protein", "uniref50_id": "UniRef50_A0AB38W8A6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8A6" ], "product": "DUF16 family-like protein" }, "genes": [], "id": "GIHJHMOALN_346", "locus": "GCA009939745_00311" }, { "type": "cds", "sequence": "contig_1", "start": 178251, "stop": 178454, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGCCAAGTACACCGCAAGGGCTCTATTGCAGAAGCAGTTAGTGCCATCCAAGCTCATGATAAGATCGTGATCTTCCACCACATTCGTCCTGATGGCGATTGTTTGGGCGCACAACACGGCTTAGCGCGTTTAATCCAAACTAACTTTCCCCACAAGCAGGTCTTCTGTGTTGGTGATCCCAAACACAACTTTCCCTGA", "aa": "MNSQVHRKGSIAEAVSAIQAHDKIVIFHHIRPDGDCLGAQHGLARLIQTNFPHKQVFCVGDPKHNFP", "aa_hexdigest": "34d9eb2d6a77d3af142893ad558c069f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7434.424999999999, "isoelectric_point": 8.807944679260256 }, "id": "GIHJHMOALN_347", "locus": "GCA009939745_00312" }, { "type": "cds", "sequence": "contig_1", "start": 178461, "stop": 178793, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGGTTTTCACTCCAAAGGAACAGATTACCCCGGAGTTAATGCAACAAGCCTTAGCCGTTATTGTTGATGCCAACTATAAGGAACGGATTGAGTGCCGGGACTTATTAGACCAAAACCAGTTTAAGGCAGTATTGCGGATTGACCACCACCCCAATGAGGACGATCTCAATACGACCCATAACTTCGTTGATGCGTCTTACATTGCCGCGGCTGAACAAGTGGTAGATCTAGCGGTGCAGGCCAAATGGAAGCTTAGCCCCCCAGCGGCTACGGCGCTGTATTTAGGTATTTATACAGATAGTAATAGGTTTCTATATAGTAATACATCATGA", "aa": "MVFTPKEQITPELMQQALAVIVDANYKERIECRDLLDQNQFKAVLRIDHHPNEDDLNTTHNFVDASYIAAAEQVVDLAVQAKWKLSPPAATALYLGIYTDSNRFLYSNTS", "aa_hexdigest": "8f0850adfe18df42c267cf5894436a9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 90, "aa_cov": 0.7454545454545455, "hmm_cov": 0.9555555555555556, "evalue": 6.094035085328097e-08, "score": 28.507266998291016, "start": 16, "stop": 97 } ], "seq_stats": { "molecular_weight": 12483.917699999985, "isoelectric_point": 4.811841392517089 }, "id": "GIHJHMOALN_348", "locus": "GCA009939745_00313" }, { "type": "cds", "sequence": "contig_1", "start": 178815, "stop": 179225, "strand": "+", "frame": 3, "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.-.-", "GO:0003676", "GO:0004527", "GO:0008441", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_P75144", "UniRef:UniRef90_P75144" ], "nt": "ATGCTATATAGAGCTCAAGCTAATATAGCTAAGATCCATGATGAGTTAAACCACACTTCCTTAAAGGACATCCAGTTTAAACAATATGTCTTTAAAAACTTTCAGACCTTTCAGAATGTTATTTACTTTGTGGCCGATAAGAAGTTCCAAAAGAAATTAAAGGTAACACCCTTAGAATGTGCACGGGTAAATATCCTAGCTAACATTGAACAATTCCACATTTGGCTGTTCTTTATAGAAGAGGGTAAGAACCACTATCGGGTCGAATTCCGTAGTAACGGAATTAACGTACGCGAAGTAGCTTTAAAGTATGGTGGCGGGGGTCATATTCAGGCCAGCGGTGCAGTTCTTAAAAGCAAGCGCGACATAATTCGTGTAGTTCAAGATTGCCAAAAGCAAATTGCTGTATAA", "aa": "MLYRAQANIAKIHDELNHTSLKDIQFKQYVFKNFQTFQNVIYFVADKKFQKKLKVTPLECARVNILANIEQFHIWLFFIEEGKNHYRVEFRSNGINVREVALKYGGGGHIQASGAVLKSKRDIIRVVQDCQKQIAV", "aa_hexdigest": "6fdbde93fb658b294c76837d7d885a14", "start_type": "ATG", "rbs_motif": null, "genes": [ "nrnA" ], "truncated": "5-prime", "pseudogene": { "start": 178515, "stop": 179225, "observations": { "mutation": [ 178791 ], "stop": [ 178791 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75144", "query_cov": 1.0, "subject_cov": 0.41975308641975306, "identity": 1.0, "score": 276.0, "evalue": 2.68e-91, "gene_start": 1, "gene_end": 136, "reference_start": 189, "reference_end": 324, "reference_sequence": "MNSQVHRKGSIAEAVSAIQAHDKIVIFHHIRPDGDCLGAQHGLARLIQTNFPHKQVFCVGDPKHNFPWLEMVFTPKEQITPELMQQALAVIVDANYKERIECRDLLDQNQFKAVLRIDHHPNEDDLNTTHNFVDASYIAAAEQVVDLAVQAKWKLSPPAATALYLGIYTDSNRFLYSNTSWRTLYLGSMLYRAQANIAKIHDELNHTSLKDIQFKQYVFKNFQTFQNVIYFVADKKFQKKLKVTPLECARVNILANIEQFHIWLFFIEEGKNHYRVEFRSNGINVREVALKYGGGGHIQASGAVLKSKRDIIRVVQDCQKQIAV" }, "paralog": false, "identity": 0.9957627118644068, "score": 471.0, "evalue": 1.63e-170, "description": "Internal stop codon at 178791. Nonsense mutation around 178791.", "psc": { "uniref90_id": "UniRef90_P75144", "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75144", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "go_ids": [ "GO:0008441", "GO:0004527", "GO:0003676" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75144", "EC:3.1.-.-", "UniRef:UniRef50_P75144", "COG:COG0618", "COG:FT", "KEGG:K06881", "GO:0008441", "GO:0004527", "GO:0003676" ] }, "pscc": { "uniref50_id": "UniRef50_P75144", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75144" ], "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA" } }, "id": "GIHJHMOALN_349", "locus": "GCA009939745_00314" }, { "type": "cds", "sequence": "contig_1", "start": 179238, "stop": 179444, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17E6AB", "UniRef:UniRef90_UPI001F17E6AB" ], "nt": "ATGCACCAAACCAAAAAAACTGCCTTGTCCAAGTCCACTTGGATTCTCATCCTCACCGCCACCGCCTCCCTCGCGACGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTAACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGA", "aa": "MHQTKKTALSKSTWILILTATASLATGLTVVGHFTSTTTTLKRQQFSYTRPNEVALRHTNAINPRLTP", "aa_hexdigest": "67f182a537085863489381967217a6d4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F17E6AB", "query_cov": 0.8823529411764706, "subject_cov": 0.8695652173913043, "identity": 0.9670000000000001, "score": 113.0, "evalue": 1.26e-31, "valid": true, "uniref50_id": "UniRef50_UPI001F17E6AB" }, "pscc": { "uniref50_id": "UniRef50_UPI001F17E6AB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17E6AB" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7523.588499999999, "isoelectric_point": 11.592334175109865 }, "id": "GIHJHMOALN_350", "locus": "GCA009939745_00315" }, { "type": "cds", "sequence": "contig_1", "start": 179511, "stop": 179768, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCGCAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNSAKGITAGSGSQQTTYDPTRTEAALTASTTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "e38d0c68fd329b9f24cab5f38593397d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 2.266225270234489e-09, "score": 32.50608444213867, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9090.854999999996, "isoelectric_point": 8.137860298156738 }, "id": "GIHJHMOALN_351", "locus": "GCA009939745_00316" }, { "type": "cds", "sequence": "contig_1", "start": 179799, "stop": 180311, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCAGACAGCACTAACCAAACAGGCGCAATGTTTGGCTTGAAGGTGAAGGATGCAACCGTGGATAGTTCGAAGCAATCAACGGAAAGCTTAAAGGGCGAAGAATCGAGTTCCAGTTCCACCACATCTTCCACCTCCACCACCCAACGTGGGGGTTCGTCAAATGAAAACAAAATCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSDSTNQTGAMFGLKVKDATVDSSKQSTESLKGEESSSSSTTSSTSTTQRGGSSNENKIKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTP", "aa_hexdigest": "ffece62585209cbedd61841a249cdcc6", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17666.820299999985, "isoelectric_point": 5.474299812316894 }, "id": "GIHJHMOALN_352", "locus": "GCA009939745_00317" }, { "type": "cds", "sequence": "contig_1", "start": 180321, "stop": 180794, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCCGACTTGGTAACATGGCCATTGCCACCAAACACCAAAAGCGCTTGCCCGAACCAGGTGAGGTTGCCAATGTTGGCGAAGTACGCTTCAAACGGGAGGGCGATGGGGGCAGGGGATTGTTCGCCTTGTTTGCGAAAGCCCTGCTTTTTATTATCGGTGTCATCTGTATCAAACCCGGTTTGGGTATTATCCGCGACCGTCTGCCCGCCATCAAACCACTCGGGGTGGCGGCGCGGGTTGAAGAACCCGGTGGTTTGGAGCAAAACATCGCGCGCCTTTCAGTCCCACAAACCGTGGTGGTTTCACTTGGGCGTTCTTCAACTGGGCGGATAGTTCGCGGTCATGGCCTTGGGATCCAAGTGATCAACGCGGTCAATGGCGGTACGGTTGCGCGCATAAGGCGCATCGTACAGAATCAGGATCGAGGCGGCGAATTTGGCGGGGTCGTTGTGAATTTGCTCAGTCGTTAA", "aa": "MGRLGNMAIATKHQKRLPEPGEVANVGEVRFKREGDGGRGLFALFAKALLFIIGVICIKPGLGIIRDRLPAIKPLGVAARVEEPGGLEQNIARLSVPQTVVVSLGRSSTGRIVRGHGLGIQVINAVNGGTVARIRRIVQNQDRGGEFGGVVVNLLSR", "aa_hexdigest": "fc51dfde4ec71012dcb8d18f4a401764", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16628.31029999998, "isoelectric_point": 11.513489341735841 }, "id": "GIHJHMOALN_353", "locus": "GCA009939745_00318" }, { "type": "cds", "sequence": "contig_1", "start": 180894, "stop": 181151, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "ATGGTCAACAAACAAACTGACGGGTTAAAGAATCTACCCTTTAACAATAACCGCTGGTTTGAATATGTACCACGGATGGCAGTTGCTGGCGCTAAGTTCGTTGGTAGGGAACTCGTTTTAGCGGGTACCATTACCATGGGTGATACCGCTACCGTACCTCGCTTACTGTACGATGAACTTGAAAGCAACCTGAACTTAGTAGCGCAAGGCCAAGGTCTTTTACGCGAAGACTTGCAACTCTTCACACCCTACGGATGA", "aa": "MVNKQTDGLKNLPFNNNRWFEYVPRMAVAGAKFVGRELVLAGTITMGDTATVPRLLYDELESNLNLVAQGQGLLREDLQLFTPYG", "aa_hexdigest": "5e7fb3bd21f7268e599038e7bfc5918b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 31, "aa_cov": 0.35294117647058826, "hmm_cov": 1.0, "evalue": 2.715488318017865e-08, "score": 28.966754913330078, "start": 1, "stop": 30 } ], "seq_stats": { "molecular_weight": 9501.790699999994, "isoelectric_point": 5.212101554870604 }, "id": "GIHJHMOALN_354", "locus": "GCA009939745_00319" }, { "type": "cds", "sequence": "contig_1", "start": 181452, "stop": 181595, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTGCTATTCTCAATCCGGAATTGTTAGCAGCTCTTCCCGACAAGGTTAAATACGGTAAGGAAAACGAGTTTGCTGCTAACGAGTACGAGCGCTTTAACCAGAAGTTAACGGTAGCTCCTACCCAAGGAACAAACTGA", "aa": "MFAAILNPELLAALPDKVKYGKENEFAANEYERFNQKLTVAPTQGTN", "aa_hexdigest": "d59c1f3728201f00d30247ea40cfac70", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5256.895600000003, "isoelectric_point": 5.1057558059692365 }, "id": "GIHJHMOALN_355", "locus": "GCA009939745_00320" }, { "type": "cds", "sequence": "contig_1", "start": 181563, "stop": 181916, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAGGGTTCTTCGAGGCGAACCGCCTGTAATCATCGTCTGACTGCCCCCGAGGGTGGCTTGGACATTCGCTTTGAGGCCGACGCCGATGTTGGAAAACGTCGGGAGAGAGGAACGCGAACCACTTGTGTTCGCACTAATTCCGCTGGACGACCCCGCGCTGTTTTGAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTGCCATACCTGTACCCATTCCCAATCCAGGGCACATAATCCAACACCTGGTCGAGCACCGACCCCACAAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGGGATCAGTTTGTTCCTTGGGTAGGAGCTACCGTTAA", "aa": "MVEGSSRRTACNHRLTAPEGGLDIRFEADADVGKRRERGTRTTCVRTNSAGRPRAVLRRGYIIHAPVVIAIPVPIPNPGHIIQHLVEHRPHKVEPGGETGKRGGEVGSVCSLGRSYR", "aa_hexdigest": "0fe593c29efb11717abe774c3ffd4463", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12733.39139999999, "isoelectric_point": 10.722526741027831 }, "id": "GIHJHMOALN_356", "locus": "GCA009939745_00321" }, { "type": "cds", "sequence": "contig_1", "start": 182013, "stop": 182186, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCCAGCAGGAACAATCAGGTACCTCCGCGGGGAATCCCGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACAGCAATGCGATGGATCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGA", "aa": "MGQQEQSGTSAGNPDSLKQDKISKSGDSLTTQDSNAMDQQEATNYTNLPPNLTPTAD", "aa_hexdigest": "536ba9928dea65cfbe785516d90a84d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6028.3012000000035, "isoelectric_point": 4.089076805114746 }, "id": "GIHJHMOALN_357", "locus": "GCA009939745_00322" }, { "type": "cds", "sequence": "contig_1", "start": 182268, "stop": 182558, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTTAATAAGTCCGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGAGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAGTGGCACAAACACAACGTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNKSGQDDNSKFKAEDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRASGSGSNTTSSPGIGFKIPEQSGTNTTSKAVLITPGLA", "aa_hexdigest": "890f22afdbdbb58513e9f1ad165ff405", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10171.074699999988, "isoelectric_point": 5.170722770690916 }, "id": "GIHJHMOALN_358", "locus": "GCA009939745_00323" }, { "type": "cds", "sequence": "contig_1", "start": 182781, "stop": 183812, "strand": "+", "frame": 3, "gene": "mgpA", "product": "adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009E1D434", "UniRef:UniRef90_UPI0009E1D434" ], "nt": "GTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAAGGGACGCCTTACCGGAGCACCCGAATGCTTTGGCGTACCAAATTAGCTATACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGTTCGGGTCAAAACAATTCGCTCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTCGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTGTTTGGAGCCATGAGTGGTAACCTCAGTAGTGTGCTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAAAAAGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGATGTGGTGGGGGTTGGTCGACTTTCTGAAAGCAACGCCGCAAAGATGAACGACGATGTTGATGGTATTGTACGCACCCCACTCGCTGAACTGTTAGATGGGGAAGGACAAACAGCTGACACTGGTCCACAAAGCGTGAAGTTCAAGTCTCCTGACCAAATTGACTTCAACCGCTTGTTTACCCACCCAGTCACCGATCTGTTTGATCCGGTAACTATGTTGGTGTATGACCAGTACATACCGCTGTTTATTGATATCCCAGCAAGTGTGAGCCCTAAAATGGTTCGTTTAAAGGTCTTGAGCTTTGACACCAACGAACAGAGCTTAGGTCTCCGCTTAGAGTTCTTTAAACCTGATCAAGATACCCAACCAAACAACAACGTTCAGGTCAATCCGAATAACGGTGACTTCTTACCACTGTTAACGGCCTCCAGTCAAGGTCCCCAAACCTTGTTTAGTCCGTTTAACCAGTGA", "aa": "MESVWDLKGVWADQAQSDSQGSTTTATRDALPEHPNALAYQISYTDKDSYKASTQGSGQNNSLYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGAMSGNLSSVLSGGGAGGGSSGSGQSGVDLSPVEKVSGWLVGQLPSTSDGNTSSTNNLAPNTNTGNDVVGVGRLSESNAAKMNDDVDGIVRTPLAELLDGEGQTADTGPQSVKFKSPDQIDFNRLFTHPVTDLFDPVTMLVYDQYIPLFIDIPASVSPKMVRLKVLSFDTNEQSLGLRLEFFKPDQDTQPNNNVQVNPNNGDFLPLLTASSQGPQTLFSPFNQ", "aa_hexdigest": "b70f8e5abc89c319dd2da4c5c5a2ac30", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpA" ], "truncated": "both", "pseudogene": { "start": 182481, "stop": 184091, "observations": { "mutation": [ 182556, 182739, 183810 ], "stop": [ 182556, 182739, 183810 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0009E1D434", "query_cov": 1.0116618075801749, "subject_cov": 0.5822147651006712, "identity": 0.9109999999999999, "score": 602.0, "evalue": 2.45e-212, "gene_start": 1, "gene_end": 343, "reference_start": 142, "reference_end": 487, "reference_sequence": "SDQTKLNLPAYGEVNGLLNPALVETYFGNTRAGGSGSNTTSSPGIGFKIPEQNNDSKATLITPGLAWTPQDVGNLVVSGTTVSFQLGGWLVTFTDFVKPRAGYLGLQLSGLNASDSDQRELIWAPRPWAAFRGSWVNRLGRVESVWDLKGVWADQAQSDSQGSTTTATRNALPEHPNALAFQVSVVEASAYKPNTSSGQTQSTNSSPYLHLVKPKKVTQSDKLDDDLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGTSSGNLSSVLSGGGAGGGSSGSGQSGVDLSPVEKVSGWLVGQLPSTSDGNTSSTNNLAPNTNTGNDVVGVGRLSESNAAKMNDDVDGIVRTPLAELLDGEGQTADTGPQSVKFKSPDQIDFNRLFTHPVTDLFDPVTMLVYDQYIPLFIDIPASVNPKMVRLKVLSFDTNEQSLGLRLEFFKPDQDTQPNNNVQVNPNNGDFLPLLTASSQGPQTLFSPFNQWPDYVLPLAITVPIVVIVLSVTLGLAIGIPMHKNKQALKAGFALSNQKVDVLTKAVGSVFKEIINRTGISQAPKRLKQTSAAKPGAPRPPVPPKPGAPKPPVQPPKKPA" }, "paralog": false, "identity": 0.9109461966604824, "score": 956.0, "evalue": 0.0, "description": "Internal stop codon at 182556, 182739, 183810. Nonsense mutation around 182556, 182739, 183810.", "psc": { "uniref90_id": "UniRef90_UPI0009E1D434", "gene": "mgpA", "product": "adhesin P1", "uniref50_id": "UniRef50_UPI0009E1D434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0009E1D434", "UniRef:UniRef50_UPI0009E1D434" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0009E1D434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009E1D434" ], "product": "adhesin P1" } }, "id": "GIHJHMOALN_359", "locus": "GCA009939745_00324" }, { "type": "cds", "sequence": "contig_1", "start": 183822, "stop": 184139, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGCCGTTAGCGATCACTGTACCTATTGTTGTGATTGTGCTCAGTGTTACCTTAGGACTTGCCATTGGAATCCCAATGCACAAGAACAAACAGGCCTTGAAGGCTGGGTTTGCGCTATCAAACCAAAAGGTTGATGTGTTGACCAAAGCGGTTGGTAGTGTCTTTAAGGAAATCATTAACCGCACAGGTATCAGTCAAGCGCCAAAACGCTTGAAACAAACCAGTGCGGCTAAACCAGGAGCACCCCGCCCACCAGTACCACCAAAGCCAGGGGCTCCTAAGCCACCAGTGCAACCACCTAAAAAACCCGCTTAG", "aa": "MLPLAITVPIVVIVLSVTLGLAIGIPMHKNKQALKAGFALSNQKVDVLTKAVGSVFKEIINRTGISQAPKRLKQTSAAKPGAPRPPVPPKPGAPKPPVQPPKKPA", "aa_hexdigest": "73882c2df7ebb5c3c334b6e0860da49d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10932.12489999998, "isoelectric_point": 11.35315647125244 }, "id": "GIHJHMOALN_360", "locus": "GCA009939745_00325" }, { "type": "cds", "sequence": "contig_1", "start": 184145, "stop": 184345, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCGAAGCTAAAGTTAAAACGTTATTTACTGTTTTTACCACTTTTACCGCTAGGGACGTTGTCACTAGCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGCGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGA", "aa": "MKSKLKLKRYLLFLPLLPLGTLSLANTYLLQDHNTLTPYTPFTTPLNGGLDVVRAAHLHPSYELVD", "aa_hexdigest": "305064b624de3835b370f99498e1956b", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7434.653600000002, "isoelectric_point": 9.157943534851075 }, "id": "GIHJHMOALN_361", "locus": "GCA009939745_00326" }, { "type": "cds", "sequence": "contig_1", "start": 184352, "stop": 184918, "strand": "+", "frame": 2, "gene": null, "product": "Putative MgpC-like protein MPN_149", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037", "UniRef:UniRef90_P75037" ], "nt": "GTGGGGGATACCAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGATCAAAGTAATACCAACCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTATGTCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGATCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGA", "aa": "MGDTKLVALVRSALVRVKFQDTTSSDQSNTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYVLIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYKTLQDLLVEQPVTPYTPNAGLARVNGVAQDTVHFGSDQESSWNSQRSQKGLKNNPGPKAVTGFKLDKGRAYRKLNES", "aa_hexdigest": "21ee961df357f32fd42cbe48d8e4c8ff", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 184316, "stop": 185221, "observations": { "mutation": [ 184343, 184916, 184976 ], "stop": [ 184343, 184916, 184976 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75037", "query_cov": 1.0106382978723405, "subject_cov": 0.4377880184331797, "identity": 0.868, "score": 304.0, "evalue": 5.86e-100, "gene_start": 1, "gene_end": 188, "reference_start": 22, "reference_end": 210, "reference_sequence": "MAVGIFILSLNPSYELVDWKRVGDTKLVALVRSALVRVKFNDGTSSDSNNQDTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLELPYVKNESGAGDSTTTNSGSLYTTLQDLLVEQPVTPYTPNAGLARVNGAAQDTVHFGSGQESSWNSQRSQKVLKNNPGPKAVTGFKLDKGRAYRKLNEAWPVYEPLDSTKDGKGKDESSWNSSEKTTAESDAPLVGSTGSQMAAVTDSQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLETWAGENKYWNRYPCCWWCFSPSSDPAIVFHKWRSTECNHPVIPYFNRPTRLLKWPDRCDGFERGTEFVVLGRGWANHVRKGYLMSPHRVELGHRQAEGVCGESAWFQWNQRHRLA" }, "paralog": false, "identity": 0.8514851485148515, "score": 492.0, "evalue": 3.66e-176, "description": "Internal stop codon at 184343, 184916, 184976. Nonsense mutation around 184343, 184916, 184976.", "psc": { "uniref90_id": "UniRef90_P75037", "product": "Putative MgpC-like protein MPN_149", "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75037", "UniRef:UniRef50_P75037" ] }, "pscc": { "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037" ], "product": "Putative MgpC-like protein MPN_149" } }, "id": "GIHJHMOALN_362", "locus": "GCA009939745_00327" }, { "type": "cds", "sequence": "contig_1", "start": 185021, "stop": 185248, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTCAACCGAGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MSSTESQMAAVTDSQQSGHNSGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "01a905cfd228e3b6c2671a39b4f01a08", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7844.479600000001, "isoelectric_point": 5.985339164733887 }, "id": "GIHJHMOALN_363", "locus": "GCA009939745_00328" }, { "type": "cds", "sequence": "contig_1", "start": 185507, "stop": 186184, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI002246AD21" ], "nt": "TTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGAGCACGGAAAACAGTGCTGCCACCAAAGACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGAACAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGAGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCACGGGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGA", "aa": "MNWGTDKQKQFVENQLGFKDDSNSSLTNFKSQGLTQPAYLIAGLDVVQDHLVFAAFKAGAVGYDMSTENSAATKDQALAWSTTAGLDSDGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSEQKATAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLNEERLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVK", "aa_hexdigest": "4a7b11d1d72df5ce47094d1e9f2897a6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002246AD21", "query_cov": 0.8711111111111111, "subject_cov": 0.8521739130434782, "identity": 0.903, "score": 340.0, "evalue": 1.65e-116, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "GIHJHMOALN_364", "locus": "GCA009939745_00329" }, { "type": "cds", "sequence": "contig_1", "start": 186212, "stop": 187600, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFF1225", "UniRef:UniRef90_UPI000AFF1225" ], "nt": "GTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGAAGGCGAGCGGAGCAGCGGAATGGGGTGAAGATTAGCACCTTGGAATCGCAAGCCACTGATGGCTTTGCCAACTCGTTACTTAACTTTGGTACCGGTCTTAAAGCCGGTGTTGACCCAGCTCCAGTAGCACGGGGTCATAAACCGAACTATAGTGCAGTACTACTAGTGCGTGGTGGCGTTGTAAGGTTAAACTTTAACCCCGATACTGATAAACTGTTGGATTCTACTGACAAAAACAGTGAACCTATCTCCTTCTCCTATACCCCATTTGGGTCTGCTGAAAGTGCCGTAGACCTCACCACGTTGAAGGATGTGACCTATATTGCTGAAAGTGGTCTGTGGTTCTATACCTTTGACAATGGTGAAAAACCAACGTACGATGGTAAACAACAACAGGTTAAAAACCGCAAGGGTTATGCTGTGATTACCGTATCACGTACCGGAATTGAATTTAACGAGGACGCTAATACCACAACCTTAAGCCAAGCCCCAGCTGCTTTGGCTGTCCAAAACGGGATTGCTTCCAGTCAGGACGACCTCACAGGCATCCTACCGTTATCCGATGAGTTCTCCGCTGTGATTACCAAGGATCAAACATGGACCGGTAAGGTTGATATCTATAAGAACACCAACGGGTTGTTTGAAAAGGATGATCAGCTATCGGAAAACGTGAAGAGGCGTGACAACGGTTTGGTCCCTATTTACAACGAAGGTATCGTCGATATTTGGGGCAGAGTGGATTTTGCTGCCAACAGTGTTTTGCAAGCGCGTAACCTCACTGATAAAACGGTTGATGAGGTGATCAATAACCCCGATATCCTCCAAAGCTTCTTTAAGTTTACCCCAGCCTTTGATAACCAAAGAGCAATGCTAGTGGGGGAAAAGACATCGGATACTACCTTAACGGTTAAACCGAAGATTGAGTACTTGGATGGTAACTTCTATGGTGAGGATTCCAAGATTGCTGGAATTCCGCTCAACATTGATTTCCCTTCCCGGATTTTTGCTGGCTTTGCTGCTTTACCGTCCTGGGTCATTCCGGTATCAGTCGGTTCATCGGTGGGCATTCTCTTAATCCTGCTCATCTTAGGCCTTGGTATTGGAATTCCAATGTATAAGGTCCGCAAGCTTCAAGACTCCAGCTTTGTTGATGTGTTTAAAAAGGTGGATACGTTGACAACCGCTGTGGGTAGCGTGTACAAGAAGATTATCACCCAAACGAGTGTGATCAAAAAAGCTCCTAGTGCGTTGAAAGCTGCTAATAACGCTGCTCCTAAAGCACCAGTTAAACCAGCTGCTCCAACAGCTCCAAGACCACCAGTCCAACCACCTAAAAAGGCTTAA", "aa": "MPYYYSANFPEAGNRRRAEQRNGVKISTLESQATDGFANSLLNFGTGLKAGVDPAPVARGHKPNYSAVLLVRGGVVRLNFNPDTDKLLDSTDKNSEPISFSYTPFGSAESAVDLTTLKDVTYIAESGLWFYTFDNGEKPTYDGKQQQVKNRKGYAVITVSRTGIEFNEDANTTTLSQAPAALAVQNGIASSQDDLTGILPLSDEFSAVITKDQTWTGKVDIYKNTNGLFEKDDQLSENVKRRDNGLVPIYNEGIVDIWGRVDFAANSVLQARNLTDKTVDEVINNPDILQSFFKFTPAFDNQRAMLVGEKTSDTTLTVKPKIEYLDGNFYGEDSKIAGIPLNIDFPSRIFAGFAALPSWVIPVSVGSSVGILLILLILGLGIGIPMYKVRKLQDSSFVDVFKKVDTLTTAVGSVYKKIITQTSVIKKAPSALKAANNAAPKAPVKPAAPTAPRPPVQPPKKA", "aa_hexdigest": "ee794a8dfd26ecf0921297c89b312275", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "5-prime", "pseudogene": { "start": 185912, "stop": 187600, "observations": { "mutation": [ 186182 ], "stop": [ 186182 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AFF1225", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 0.998, "score": 855.0, "evalue": 8.33e-309, "gene_start": 1, "gene_end": 462, "reference_start": 232, "reference_end": 693, "reference_sequence": "AFKAGAVGYDMTTDSSASTYNQALAWSTTAGLDSDGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSDQKATAKILNGPINDLFTLLDTFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLNEERLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVKWTNTASHYLPVPYYYSANFPEAGNRRRAEQRNGVKISTLESQATDGFANSLLNFGTGLKAGVDPAPVARGHKPNYSAVLLVRGGVVRLNFNPDTDKLLDSTDKNSEPISFSYTPFGSAESAVDLTTLKDVTYIAESGLWFYTFDNGEKPTYDGKQQQVKNRKGYAVITVSRTGIEFNEDANTTTLSQAPAALAVQNGIASSQDDLTGILPLSDEFSAVITKDQTWTGKVDIYKNTNGLFEKDDQLSENVKRRDNGLVPIYNEGIVDIWGRVDFAANSVLQARNLTDKTVDEVINNPDILQSFFKFTPAFDNQRAMLVGEKTSDTTLTVKPKIEYLDGNFYGEDSKIAGIPLNIDFPSRIFAGFAALPSWVIPVSVGSSVGILLILLILGLGIGIPMYKVRKLQDSSFVDVFKKVDTLTTAVGSVYKKIITQTSVIKKAPSALKAANNAAPKAPVKPAAPTAPRPPVQPPKKA" }, "paralog": false, "identity": 0.9150326797385621, "score": 1036.0, "evalue": 0.0, "description": "Internal stop codon at 186182. Nonsense mutation around 186182.", "psc": { "uniref90_id": "UniRef90_UPI000AFF1225", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI000AFF1225", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AFF1225", "UniRef:UniRef50_UPI000AFF1225" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AFF1225", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFF1225" ], "product": "MgpC family cytadherence protein" } }, "id": "GIHJHMOALN_365", "locus": "GCA009939745_00330" }, { "type": "cds", "sequence": "contig_1", "start": 187717, "stop": 187998, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_143", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75143", "UniRef:UniRef90_P75143" ], "nt": "ATGAGTCATAAAGATTTTAATGGGCTTCAAGCCCCTCAGTTGTTAAGTTCGAGTTCCCCTGTTGCAAAGAAACAAAGTAGTCATAAACTCAGGCATGCCTTAAAGCACGCCCGTTACTTAAACCATAGTAGTAAGCGTACCCTCAAGCATGCCTTAGAGCTGCATGAAGACAACCAGGTCTTACTGGAAAAAGAAGGTAGTCCTAACTTCCAGGACTGGCTTTCTAAGCAACCTGGCGTCAATAAAACCAGCCTTAAGTACAACAAAAGCTTGGGTAGTTGA", "aa": "MSHKDFNGLQAPQLLSSSSPVAKKQSSHKLRHALKHARYLNHSSKRTLKHALELHEDNQVLLEKEGSPNFQDWLSKQPGVNKTSLKYNKSLGS", "aa_hexdigest": "6fb5f06c63730a34196b2433ee85fb01", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 187717, "stop": 188244, "observations": { "mutation": [ 187996 ], "stop": [ 187996 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75143", "query_cov": 1.0, "subject_cov": 0.5314285714285715, "identity": 1.0, "score": 179.0, "evalue": 3.88e-56, "gene_start": 1, "gene_end": 93, "reference_start": 1, "reference_end": 93, "reference_sequence": "MSHKDFNGLQAPQLLSSSSPVAKKQSSHKLRHALKHARYLNHSSKRTLKHALELHEDNQVLLEKEGSPNFQDWLSKQPGVNKTSLKYNKSLGSWISKESKPKKRFPPYFTYKGSKTTPEEAKALQQMKQSQKRFFHENMHSFLNEVAHNPMIQRFKQKQAKRAANTRQRTYKYRQ" }, "paralog": false, "identity": 0.9828571428571429, "score": 348.0, "evalue": 5.39e-125, "description": "Internal stop codon at 187996. Nonsense mutation around 187996.", "psc": { "uniref90_id": "UniRef90_P75143", "product": "Uncharacterized protein MPN_143", "uniref50_id": "UniRef50_P75143", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75143", "UniRef:UniRef50_P75143" ] }, "pscc": { "uniref50_id": "UniRef50_P75143", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75143" ], "product": "Uncharacterized protein MPN_143" } }, "id": "GIHJHMOALN_366", "locus": "GCA009939745_00331" }, { "type": "cds", "sequence": "contig_1", "start": 188095, "stop": 188244, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAATCCCAAAAGCGCTTCTTTCACGAAAATATGCACAGCTTTCTCAATGAAGTTGCACATAACCCAATGATCCAGCGCTTTAATCAAAAGCAGGCCAAACGGGCTGCTAATACGCGACAACGCACTTACAAATACCGTCAGTAA", "aa": "MKQSQKRFFHENMHSFLNEVAHNPMIQRFNQKQAKRAANTRQRTYKYRQ", "aa_hexdigest": "97ec09b9e2f3be8f4311b6b0653e5851", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6124.919300000003, "isoelectric_point": 11.422459983825682 }, "id": "GIHJHMOALN_367", "locus": "GCA009939745_00332" }, { "type": "cds", "sequence": "contig_1", "start": 188247, "stop": 188378, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W9U9", "UniRef:UniRef90_A0AB38W9U9" ], "nt": "ATGCACAAGATTAAACACTATAAACCCTTAAAGCGCAAGACCTGGTTAAACAAGGTTTCCAAGCAACGCCGCGAGCGCATGTCCGCTAAGTGCTATCAACCACCTAAGAGAGAAGTGATGCAGCCAACTTGA", "aa": "MHKIKHYKPLKRKTWLNKVSKQRRERMSAKCYQPPKREVMQPT", "aa_hexdigest": "7feeb616217eaab4132de434a7c43250", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W9U9", "query_cov": 1.0, "subject_cov": 0.9347826086956522, "identity": 1.0, "score": 86.7, "evalue": 8.35e-22, "valid": true, "uniref50_id": "UniRef50_A0AB38W9U9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W9U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W9U9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5366.414400000002, "isoelectric_point": 10.8630033493042 }, "id": "GIHJHMOALN_368", "locus": "GCA009939745_00333" }, { "type": "cds", "sequence": "contig_1", "start": 188350, "stop": 188718, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAGGGTTCTTCAAGGCGAACCGCCCACAGTCGTCGTCTGACTGCCCCCGAGGGTGGCTTGGACATTCGCTTTGAGGCCAACGCCGATGTTGGAAAACGTCGGGAAAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCTGGTTTGAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTCCCATACCTGTACCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCCAGAACAGACCCCACAAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGGGATCAGGTGGTGCTAACTCTTTCAAGTTGGCTGCATCACTTCTCTCTTAG", "aa": "MVEGSSRRTAHSRRLTAPEGGLDIRFEANADVGKRREKGTRTTCVRTSSAGRPRAGLRRGYIIHAPVVIPIPVPIPNPGNIIQHLVQNRPHKVEPGGETGKRGGEVGSGGANSFKLAASLLS", "aa_hexdigest": "bf8e6c0e0c21f2e3291fed050395feef", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12935.592499999986, "isoelectric_point": 11.435998344421389 }, "id": "GIHJHMOALN_369", "locus": "GCA009939745_00334" }, { "type": "cds", "sequence": "contig_1", "start": 188818, "stop": 188991, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCAGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGACCGGTCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGA", "aa": "MGQQGQSGTSAGNPDSLKQDKISKSGDSLTTQDGNATGQQEATNYTNLPPNLTPTAD", "aa_hexdigest": "05af81cce8b055a65f0b9ea419c5cb62", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5838.084300000002, "isoelectric_point": 4.306201744079591 }, "id": "GIHJHMOALN_370", "locus": "GCA009939745_00335" }, { "type": "cds", "sequence": "contig_1", "start": 189073, "stop": 189351, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNRSGSDSNKFQATDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRAGGSGSNTTSSPGIGFKIPEQNNDSKATLITPGLA", "aa_hexdigest": "39714ed164769acd21b8843301f92ef5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9768.59219999999, "isoelectric_point": 5.086487388610839 }, "id": "GIHJHMOALN_371", "locus": "GCA009939745_00336" }, { "type": "cds", "sequence": "contig_1", "start": 189574, "stop": 189879, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD7934", "PFAM:PF03257.18" ], "nt": "GTGGAGAGTGTGTGGGATTTAAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTGAAGCTTCCGGGTCAGCTTTGGCACCGCACCCGAATGCTTTGGCGTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAGCTCTTCAACCTCAAGTTCGGGTTCCGGGTCAAGTTCAAACACCTCCCCCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCTAA", "aa": "MESVWDLKGVWADQAQLAAQAATSEASGSALAPHPNALAFQVSVVEASAYSSSTSSSGSGSSSNTSPYLHLIKPKKVESTTQLDQGLKNLLDPNQVRTKLR", "aa_hexdigest": "7e44cf76d5fe8a97612c72a3c3bea716", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034DD7934", "query_cov": 0.8415841584158416, "subject_cov": 0.9883720930232558, "identity": 0.541, "score": 79.0, "evalue": 2.64e-17, "valid": false, "uniref50_id": "UniRef50_UPI0034DD7934" }, "pscc": { "uniref50_id": "UniRef50_UPI0034DD7934", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD7934" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 56, "aa_cov": 0.5544554455445545, "hmm_cov": 1.0, "evalue": 8.855709360434896e-28, "score": 92.62002563476562, "start": 46, "stop": 101 } ], "seq_stats": { "molecular_weight": 10601.623699999987, "isoelectric_point": 6.7117414474487305 }, "id": "GIHJHMOALN_372", "locus": "GCA009939745_00337" }, { "type": "cds", "sequence": "contig_1", "start": 190436, "stop": 190975, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_145", "db_xrefs": [ "RefSeq:WP_014325374.1", "SO:0001217", "UniParc:UPI0001DD64CE", "UniRef:UniRef100_A0AAX0SQH7", "UniRef:UniRef50_P75141", "UniRef:UniRef90_P75141" ], "nt": "ATGTCTTACTCACCTTCACTTAAGGAAATTATTGCTATTTTGCAAAAATACACTTCGAAAAACTACCAGTCAAATTGCAAAACTAGACCCGATGGTAAGCTTGAATTATCATTAAATGGAGTTTTTGAAGAAATCGTGAAAACACCCGGTAAAACTAGGTACGTAACCCATAAACAGCTTGATCAGAAATTAAAAGATTTCAAGCAAGATCTGATGGTTGAGTTGCACGACACGTTTGCTACTAAAACTGATCTTAAAGAATCGGAAGCACGCATTAACCAAAAGTTAGAAGCACTAATTCAAATTGTTATGGTTCAGGGTGAGCAGATAAAAGTTCACGGCGAGCAAATTAACAAACTGACTCAAACTGTTGAAAAACAGGGCGAGAAAATTGAAGCTCAAGGACAACAAATTCAAAAAGTTAATGAAACTTTGAATTTTGTAGTTGAAAGCCTTGGATCTATCCACAAAAGACTCGATTCAATGGAAGGCCGCTTGGACTCAATGGAAAACCGTCTTGACAAACTAGAGTCCAAGTAG", "aa": "MSYSPSLKEIIAILQKYTSKNYQSNCKTRPDGKLELSLNGVFEEIVKTPGKTRYVTHKQLDQKLKDFKQDLMVELHDTFATKTDLKESEARINQKLEALIQIVMVQGEQIKVHGEQINKLTQTVEKQGEKIEAQGQQIQKVNETLNFVVESLGSIHKRLDSMEGRLDSMENRLDKLESK", "aa_hexdigest": "45147d98a56f0de92a7f04d6ba2c62a5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64CE", "ncbi_nrp_id": "WP_014325374.1", "uniref100_id": "UniRef100_A0AAX0SQH7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64CE", "RefSeq:WP_014325374.1", "UniRef:UniRef100_A0AAX0SQH7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQH7", "uniref90_id": "UniRef90_P75141" }, "psc": { "uniref90_id": "UniRef90_P75141", "product": "UPF0134 protein MPN_145", "uniref50_id": "UniRef50_P75141" }, "pscc": { "uniref50_id": "UniRef50_P75141", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75141" ], "product": "UPF0134 protein MPN_145" }, "genes": [], "id": "GIHJHMOALN_373", "locus": "GCA009939745_00338" }, { "type": "cds", "sequence": "contig_1", "start": 191241, "stop": 191861, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_146", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140", "UniRef:UniRef90_P75140" ], "nt": "ATGCGATTTAAATATGGTGCAGTTGCTTTTAGTGGCTTTTTAGGTCTAGCAGTGATGTTGGCTAGTTGTGGTGCTAAAGGGCGTTTTGACCAGGTTGATGATGGCAAAATTAAGCTAGCTTCTTCACTTACCGGTAAGCGCGATGTGATACTGAAAGAAGTGCTCAACAAATATAACAGTCGCAAAGCCAAGGACGATTATCCGATTGAAATAACCAAAATAGCCGGTAGCTATGATGGTGGTCGATCTGATCTGCAAACGCGGTTGAGTGTCAAGGATAAAACAACCTTTTACAACATGATCTTGAATTATTCTGACACCATTTCTACTTTAGGTCGCAGTAACATGGAACTACCATTAGATAGCGTTGACGTATCACAGTTTTCAGAAAACTTCTTGAGCTTTAATGACCGCATAAGTGGTATTTCCAGAAAAGGCATTTACGGCATTCCGGTATCAATGTCAACTGATATCCTAGTAATCAACGGGCCGGTATTGCACTATATTTTGAATAGTGCAAAAAAGAAAGATGGTGCAGTAACTAAGAAAAACGCAAGTAATTCCAATGGTAACGAAGGAACGTTAACAGTAAATAACGATCAACAAACCACTGAACTATGA", "aa": "MRFKYGAVAFSGFLGLAVMLASCGAKGRFDQVDDGKIKLASSLTGKRDVILKEVLNKYNSRKAKDDYPIEITKIAGSYDGGRSDLQTRLSVKDKTTFYNMILNYSDTISTLGRSNMELPLDSVDVSQFSENFLSFNDRISGISRKGIYGIPVSMSTDILVINGPVLHYILNSAKKKDGAVTKKNASNSNGNEGTLTVNNDQQTTEL", "aa_hexdigest": "6386f4654261516e21c20b60a87aab8c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 191241, "stop": 192092, "observations": { "mutation": [ 191859, 192021 ], "stop": [ 191859, 192021 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75140", "query_cov": 1.0, "subject_cov": 0.7279151943462897, "identity": 1.0, "score": 324.0, "evalue": 1.19e-109, "gene_start": 1, "gene_end": 206, "reference_start": 1, "reference_end": 206, "reference_sequence": "MRFKYGAVAFSGFLGLAVMLASCGAKGRFDQVDDGKIKLASSLTGKRDVILKEVLNKYNSRKAKDDYPIEITKIAGSYDGGRSDLQTRLSVKDKTTFYNMILNYSDTISTLGRSNMELPLDSVDVSQFSENFLSFNDRISGISRKGIYGIPVSMSTDILVINGPVLHYILNSAKKKDGAVTKKNASNSNGNEGTLTVNNDQQTTELWKKIEEAAKTNGKTTQEQTKRDAKQSTSLIQLKEGSANTTEGNASESDKEIKKSWGNYQEVDGGLKGYTFKASVFEN" }, "paralog": false, "identity": 0.9929328621908127, "score": 479.0, "evalue": 4.85e-173, "description": "Internal stop codon at 191859, 192021. Nonsense mutation around 191859, 192021.", "psc": { "uniref90_id": "UniRef90_P75140", "product": "Uncharacterized protein MPN_146", "uniref50_id": "UniRef50_P75140", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75140", "UniRef:UniRef50_P75140" ] }, "pscc": { "uniref50_id": "UniRef50_P75140", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140" ], "product": "Uncharacterized protein MPN_146" } }, "id": "GIHJHMOALN_374", "locus": "GCA009939745_00339" }, { "type": "cds", "sequence": "contig_1", "start": 192099, "stop": 193184, "strand": "+", "frame": 3, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75139", "UniRef:UniRef90_P75139" ], "nt": "ATGGTTGATTTTGCCACTAGGGTAGCGAAATCATTTAAGGATAAAGTTAGTGGTATCGATAATAAAAAAGGCACTGACATCCAAGGTGTTTTAGGTCTAGACAGTACACCAAACGTTTTGTTCGCTTCAGTATTTGCAGCTGGTGGTGGTAGCTATGACAACTTCTTTTACAAGGTAGAAAATGGCCGGGCTGATTTCCGTAATTTTGCAAATAAAGGTACTTCTTACAAAAACTTAGAGAAGGTTTACAACGATTACAAGAATTTAATCGGTAGCAACGGTCTTTTTGCTAGCAAAGATGGCTCTTACTCATCTAACTTTGAAAAGTTCCACCAGTTAGCTTTTTATGTAGGTTCTAGCTCTGGTTATAACTATGCTTTTGCTGATGAAACTGCTAAACGGTTAAAGTTTGGTGATAGTGCCATTGAGTATCCTAACGATACGCTTGAAATTAAGGCACCGACAAATAATAGTCAAAGTGGTGATGGGAATGGTGGCACCAATAACGACAATCTCTTAGGTACTTTTGACATTAGGGAAAAGAGTAACGGCAAAAAAGGTGAGAGCAACGGTAAACAAGGAAATGGTCAAGATAAAAAGACAATTTCGCTTTATAAAGATTCGATTCCTAAAGAAAAAACTGAAGGCACTGATGCAATTCTAATTAGCGATAATCAACTCATTAATCAATTACAAACTGCCGCTAAAACTAGTAGTTCACAAAATAAAGCTAACACTGCAGCTATAACTTTTAAGCAAAGCAATAAAAGTGAAACTGACCAAAGTACAACTAGTCAGTTTATCGGCTACACAACAACTGCTTCATTGAAAGCTGATAAGAAAAATATCTTTGATGTGAAGAAGCTAAATAACGAAAAGTCTGAGCGCAAAATCATTGTCGGTGCCACGGTGGAAACCTTAAATCAAGCAAACACATTGCAAGCTAATGAAGCCATTATTAAACCAGCACCTGGTAAGTACCAAAGTACTGATTCACACAAGGTCATGATTACTCAAGGCCCGAATATTGTCGGAATTCATGCCAACGAAAAAGAGGACAAAGAAACACAAAAGTTTATTAACTGA", "aa": "MVDFATRVAKSFKDKVSGIDNKKGTDIQGVLGLDSTPNVLFASVFAAGGGSYDNFFYKVENGRADFRNFANKGTSYKNLEKVYNDYKNLIGSNGLFASKDGSYSSNFEKFHQLAFYVGSSSGYNYAFADETAKRLKFGDSAIEYPNDTLEIKAPTNNSQSGDGNGGTNNDNLLGTFDIREKSNGKKGESNGKQGNGQDKKTISLYKDSIPKEKTEGTDAILISDNQLINQLQTAAKTSSSQNKANTAAITFKQSNKSETDQSTTSQFIGYTTTASLKADKKNIFDVKKLNNEKSERKIIVGATVETLNQANTLQANEAIIKPAPGKYQSTDSHKVMITQGPNIVGIHANEKEDKETQKFIN", "aa_hexdigest": "4b2ea03af66f97c746952e82af37566d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 192099, "stop": 193487, "observations": { "mutation": [ 193182, 193206 ], "stop": [ 193182, 193206 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75139", "query_cov": 1.0, "subject_cov": 0.7443298969072165, "identity": 0.997, "score": 674.0, "evalue": 5.08e-242, "gene_start": 1, "gene_end": 361, "reference_start": 1, "reference_end": 361, "reference_sequence": "MVDFATRVAKSFKDKVSGIDNKKGTDIQGVLGLDSTPNVLFASVFAAGGGSYDNFFYKVENGRADFRNFANKGTSYKNLEKVYNDYKNLIGSNGLFASKDGSYSSNFEKFHQLAFYVGSSSGYNYAFADETAKRLKFGDSAIEYPNDTLEIKAPTNNSQSGDGNGGTNNDNLLGTFDIREKSNGKKGESNGKQGNGQDKKTISLYKDSIPKEKTEGTDAILISDNQLINQLQTAAKTSSSQNKANTAAITFKQSNKSETDQSTTSQVIGYTTTASLKADKKNIFDVKKLNNEKSERKIIVGATVETLNQANTLQANEAIIKPAPGKYQSTDSHKVMITQGPNIVGIHANEKEDKETQKFINWYLNKEESWSVQNSGSTTTKKQTAAQYFAEQSSYITPLKNNFKADQSATKDSTVNTNYFTKQTFDLFKEVNDGKVLGFNDPSDFRSGKFRNTIGSTFNATISSKVDFNKFFENFKASLGSGFER" }, "paralog": false, "identity": 0.9935064935064936, "score": 894.0, "evalue": 0.0, "description": "Internal stop codon at 193182, 193206. Nonsense mutation around 193182, 193206.", "psc": { "uniref90_id": "UniRef90_P75139", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75139", "go_ids": [ "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75139", "UniRef:UniRef50_P75139", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75139", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75139" ], "product": "Uncharacterized protein MPN_147" } }, "id": "GIHJHMOALN_375", "locus": "GCA009939745_00340" }, { "type": "cds", "sequence": "contig_1", "start": 193212, "stop": 193556, "strand": "+", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_098", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594" ], "nt": "GTGCAAAACAGCGGTTCAACTACAACTAAAAAGCAAACTGCTGCTCAATATTTTGCCGAACAGTCCTCTTACATCACTCCTTTAAAAAATAACTTTAAAGCAGATCAAAGTGCAACAAAAGATAGCACTGTTAACACTAACTATTTCACTAAACAAACATTTGATCTCTTCAAAGAAGTTAATGATGGAAAAGTGCTTGGTTTTAATGATCCAAGTGACTTTAGGAGTGGTAAGTTCCGCAATACTATTGGTTCTACTTTTAACGCAACCATCAGTTCTAAAGTTGACTTTAACAAGTTTTTTGAAAACTTTAAAGCTTCTTTAGGGTCTGGTTTTGAGCGTTAA", "aa": "MQNSGSTTTKKQTAAQYFAEQSSYITPLKNNFKADQSATKDSTVNTNYFTKQTFDLFKEVNDGKVLGFNDPSDFRSGKFRNTIGSTFNATISSKVDFNKFFENFKASLGSGFER", "aa_hexdigest": "d4ca02fa10a084a1bd117b29b5139ba6", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XG79", "query_cov": 0.9385964912280702, "subject_cov": 0.8492063492063492, "identity": 0.523, "score": 110.0, "evalue": 4.69e-29, "valid": false, "product": "Lipoprotein", "uniref50_id": "UniRef50_P75594" }, "pscc": { "uniref50_id": "UniRef50_P75594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594" ], "product": "Uncharacterized lipoprotein MPN_098" }, "genes": [], "id": "GIHJHMOALN_376", "locus": "GCA009939745_00341" }, { "type": "cds", "sequence": "contig_1", "start": 193721, "stop": 194005, "strand": "+", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGCACAAAAAGAAGTTTTTATCAAGAACTTCCTTTGCTTCTTTGTTTTTGCTTTTAGGAACAGTATTGAGTGCTTGTAGCAAAGTAGAGTCCACAAAAAGTGTTCATCCTGTAAAAAGTTTTAAATCTGATCTAAAAAGTCTTCTTAAAGAAACAATTAAACAAGATATTGATTTATCTAAAACAATCAAAACTTCAGAAGGCAAACAACAGATAATAACTTCTTTAAAAAAGTCTTACGGAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCATGA", "aa": "MHKKKFLSRTSFASLFLLLGTVLSACSKVESTKSVHPVKSFKSDLKSLLKETIKQDIDLSKTIKTSEGKQQIITSLKKSYGVNPKDTTKLLLDA", "aa_hexdigest": "fa1f03d69dc5fd69fe4f6f3ed7b80375", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.9306930693069307, "identity": 0.606, "score": 100.0, "evalue": 9.49e-26, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "GIHJHMOALN_377", "locus": "GCA009939745_00342" }, { "type": "cds", "sequence": "contig_1", "start": 194090, "stop": 194179, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGGTTGATCATTTCCAAATGACATACCAAAGTTTTAAGGATTTATCAATTAAAGCTAAGTTGAGCTATACCTTTAATTGA", "aa": "MERKVDHFQMTYQSFKDLSIKAKLSYTFN", "aa_hexdigest": "893f67d2558641e21e4c66ec2ae765db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3557.0605000000005, "isoelectric_point": 9.302803993225098 }, "id": "GIHJHMOALN_378", "locus": "GCA009939745_00343" }, { "type": "cds", "sequence": "contig_1", "start": 194505, "stop": 194651, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64D2", "UniRef:UniRef100_A0AB38W7S6", "UniRef:UniRef50_A0AB38W7S6", "UniRef:UniRef90_A0AB38W7S6" ], "nt": "ATGAGCTTTTGGAAGTGTCAACAAAATTGCAAAAAAGTTTTTGATGAAATTAATTTGAATAAAAATAAAAAGCCATCTGTTCAAGGCGAAAAGCAAGTGGCTAAAATTTTTCCAAGTACAACCAAGCAATCACAACAAATTACCTAA", "aa": "MSFWKCQQNCKKVFDEINLNKNKKPSVQGEKQVAKIFPSTTKQSQQIT", "aa_hexdigest": "dc6a5950de81f626c12ae291ce84ec9f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64D2", "uniref100_id": "UniRef100_A0AB38W7S6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64D2", "UniRef:UniRef100_A0AB38W7S6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7S6", "uniref90_id": "UniRef90_A0AB38W7S6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W7S6", "uniref50_id": "UniRef50_A0AB38W7S6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7S6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7S6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5587.413200000003, "isoelectric_point": 9.7619478225708 }, "id": "GIHJHMOALN_379", "locus": "GCA009939745_00344" }, { "type": "cds", "sequence": "contig_1", "start": 194832, "stop": 195038, "strand": "+", "frame": 3, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4", "UniRef:UniRef90_A0AAP8E4D4" ], "nt": "ATGATAGAAGTATTAAGTGATTCTGAAATTTTATCTTATTTATTAAGTAATAAATACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAG", "aa": "MIEVLSDSEILSYLLSNKYTADGVNEIYVTMNIKDKDSNTQRKPDKMSLELELFLNQFIEFLKEQRKS", "aa_hexdigest": "1c2b98a315da9777c8492c7fded3d0bd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAP8E4D4", "query_cov": 1.0, "subject_cov": 0.8095238095238095, "identity": 1.0, "score": 130.0, "evalue": 3.71e-38, "valid": true, "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D4" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4" ], "product": "Family K-like protein" }, "genes": [], "id": "GIHJHMOALN_380", "locus": "GCA009939745_00345" }, { "type": "cds", "sequence": "contig_1", "start": 195128, "stop": 195697, "strand": "+", "frame": 2, "gene": null, "product": "Putative MgpC-like protein MPN_149", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037", "UniRef:UniRef90_P75037" ], "nt": "GTGGGTGATACCAAGTTAGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTAATGATGGGACGAGTTCGGATTCTAATAACCAAGATACGAATCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTAGAGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGCTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGTCCTTAAAAACAACCCCGGACCCAAAGTCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTAAATGAAGCTTGA", "aa": "MGDTKLVALVRSALVRVKFNDGTSSDSNNQDTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLELPYVKNESGAGDSTTTNSGSLYTTLQDLLVEQPVTPYTPNAGLARVNGAAQDTVHFGSGQESSWNSQRSQKVLKNNPGPKVVTGFKLDKGRAYRKLNEA", "aa_hexdigest": "ceca23a010009fdea17d89071a801007", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 195065, "stop": 196000, "observations": { "mutation": [ 195119, 195695 ], "stop": [ 195119, 195695 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75037", "query_cov": 1.0, "subject_cov": 0.43548387096774194, "identity": 0.9890000000000001, "score": 357.0, "evalue": 5.43e-121, "gene_start": 1, "gene_end": 189, "reference_start": 22, "reference_end": 210, "reference_sequence": "MAVGIFILSLNPSYELVDWKRVGDTKLVALVRSALVRVKFNDGTSSDSNNQDTNQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLELPYVKNESGAGDSTTTNSGSLYTTLQDLLVEQPVTPYTPNAGLARVNGAAQDTVHFGSGQESSWNSQRSQKVLKNNPGPKAVTGFKLDKGRAYRKLNEAWPVYEPLDSTKDGKGKDESSWNSSEKTTAESDAPLVGSTGSQMAAVTDSQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLETWAGENKYWNRYPCCWWCFSPSSDPAIVFHKWRSTECNHPVIPYFNRPTRLLKWPDRCDGFERGTEFVVLGRGWANHVRKGYLMSPHRVELGHRQAEGVCGESAWFQWNQRHRLA" }, "paralog": false, "identity": 0.9903536977491961, "score": 603.0, "evalue": 6.31e-220, "description": "Internal stop codon at 195119, 195695. Nonsense mutation around 195119, 195695.", "psc": { "uniref90_id": "UniRef90_P75037", "product": "Putative MgpC-like protein MPN_149", "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75037", "UniRef:UniRef50_P75037" ] }, "pscc": { "uniref50_id": "UniRef50_P75037", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75037" ], "product": "Putative MgpC-like protein MPN_149" } }, "id": "GIHJHMOALN_381", "locus": "GCA009939745_00346" }, { "type": "cds", "sequence": "contig_1", "start": 195701, "stop": 196117, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAACCCCTGGATTCGACCAAGGATGGCAAGGGGAAGGATGAGAGCTCTTGGAACAGTTCGGAAAAAACAACGGCGGAAAGTGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATACCTCAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGGGCGGGGGAAAACAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCCACAAATGA", "aa": "MYEPLDSTKDGKGKDESSWNSSEKTTAESDAPLVGSTGSQMAAVTDSQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLETWAGENKYWNRYPCCWWCFSPSSDPAIVFHK", "aa_hexdigest": "1088188cd4fd0a9a1f87456e227f457c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14993.192999999997, "isoelectric_point": 5.013051414489746 }, "id": "GIHJHMOALN_382", "locus": "GCA009939745_00347" }, { "type": "cds", "sequence": "contig_1", "start": 196287, "stop": 196841, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "nt": "TTGAACTGGGGCACCGACAAGCAGAAGGAGTTTGTGGAGAATCAGCTTGGTTTCAATGAAACCAGCGCCACCGACTCGCATAACTTTAAAAAAGCGCTGTTACATCAACCCGCCTACCTCATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTCGAGCCGGCGCGGTGGGGTATGATATGACGACTGATACGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAACGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACGACTTATCCGGTCAAGTCCGACCAAAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATAAAAAAAGCCATCCACCTAAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACGATTGA", "aa": "MNWGTDKQKEFVENQLGFNETSATDSHNFKKALLHQPAYLISGLDVVADHLVFAAFRAGAVGYDMTTDTNASTYNQALTWSTTAGLDSAGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSKNTEAVQTTYPVKSDQKASAKIASLINASPLNSYGDKKSHPPKVKGRQLQNIFDD", "aa_hexdigest": "ebb441199d47eb2d0462a23a5d0b4aa5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCE6DDF", "query_cov": 1.0054347826086956, "subject_cov": 0.8409090909090909, "identity": 0.789, "score": 279.0, "evalue": 2.59e-93, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "GIHJHMOALN_383", "locus": "GCA009939745_00348" }, { "type": "cds", "sequence": "contig_1", "start": 197273, "stop": 197755, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_151", "db_xrefs": [ "RefSeq:WP_014574897.1", "SO:0001217", "UniParc:UPI0001DD64D4", "UniRef:UniRef100_A0AAX0SNI3", "UniRef:UniRef50_P75490", "UniRef:UniRef90_P75035" ], "nt": "TTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGAATTTAATGGCAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAACGAATATAAAGACTCAAACGACCAACGGCTCATAAAGATTGAAACTACTTTGGCTGCCCAAGGTGAGCAAATTAGTCAGCTAGTTCAAATTGTGTTCCTCCAAGGAAAACAAATTAAAGAACTTCAAGCGGAGCAAAAAGCACAAAGAGTTGAATTTAACGCGCGCATGGATCGCTTTGAAAGCCTTGTGCTTAAGAGTCTGGAATCAATCGGAAACACTTTGACTGATTTTGGTAAACGACTCGATTCAATGGAAACTCGTTTGGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTAGACAAAATCGATCCACCTAAATAG", "aa": "MFKKRLNKDKINNCHVWEEELPDGSYDMEFNGNLNHMEKRKSGYVTQKQFNEYKDSNDQRLIKIETTLAAQGEQISQLVQIVFLQGKQIKELQAEQKAQRVEFNARMDRFESLVLKSLESIGNTLTDFGKRLDSMETRLDSMDGRLDSMETRLDKIDPPK", "aa_hexdigest": "1e7763080aa0ad97420f12daf9c242b6", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64D4", "ncbi_nrp_id": "WP_014574897.1", "uniref100_id": "UniRef100_A0AAX0SNI3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64D4", "RefSeq:WP_014574897.1", "UniRef:UniRef100_A0AAX0SNI3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SNI3", "uniref90_id": "UniRef90_P75035" }, "psc": { "uniref90_id": "UniRef90_P75035", "product": "UPF0134 protein MPN_151", "uniref50_id": "UniRef50_P75490" }, "pscc": { "uniref50_id": "UniRef50_P75490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75490" ], "product": "UPF0134 protein MPN_287" }, "genes": [], "id": "GIHJHMOALN_384", "locus": "GCA009939745_00349" }, { "type": "cds", "sequence": "contig_1", "start": 198039, "stop": 198689, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTAAGTACGGCGCTATTGTTTTTAGTTGTCTTTTGGGCGTTTCGGCCATTTTAGCTGCCTGTGGGGCAAGGGGCAAATTTAACCAAGTTGATGATGGCAAGATTAAGCTAGCTTCATCTTTAACTTCCAGAAGTGCGTCAACAGCTTTACAAAAAGTTGTTGAAAAGTACAACAAGGTTAAAGGCGTAAATGATTACCCCATTGAAATTACCCAAATTGCTGGCGGTTATGATGGCGGGCGGACTGATCTACAAACCCGAGTTAACGTTAAAGACACGACTAATTTTTATAACCTCATCTTGAACTATCCCGACTTAGTTTCCACTTTGGGACGTGTTGGGATGGAACTACCTTTTGATAAGGTTAAAACTGATAAGTTAGCTGACCGTTTTTTGGATTTCAACAACCGTATTAGTGCCATTTCCAAAAAGGGCATTTATGGCATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATTAATGGACCCGTTTTGCACTATATTTTGAACAGTGCACAAGGAAAGGTTAGTTCTGTCACTTCGGTGCAAAGAACAGCAGCTTCTGGTAGTGGAACAACAAATAATAATGGAGTAACAAAACCTTTGAAGATTGATAAAGAAAATGAATCTACAAAGAAACTTTGA", "aa": "MKFKYGAIVFSCLLGVSAILAACGARGKFNQVDDGKIKLASSLTSRSASTALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELPFDKVKTDKLADRFLDFNNRISAISKKGIYGIPVSLSTEVLSINGPVLHYILNSAQGKVSSVTSVQRTAASGSGTTNNNGVTKPLKIDKENESTKKL", "aa_hexdigest": "ea40edbbeed660d617dde646146b80b4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23288.397800000013, "isoelectric_point": 9.607932853698731 }, "id": "GIHJHMOALN_385", "locus": "GCA009939745_00350" }, { "type": "cds", "sequence": "contig_1", "start": 198774, "stop": 198917, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCACTCAAACTCAACCTACTGACAATAGTAACGATGCTAATCAAAGCGACCAGGCGATAGAAAAGACTTGGGGTAAATATCAGGAGGTTGAAGGTGGTTTAAAAGATTACACCTTCAAAGCCAGTGTGTTTGAAAACTGA", "aa": "MSTQTQPTDNSNDANQSDQAIEKTWGKYQEVEGGLKDYTFKASVFEN", "aa_hexdigest": "20fa1cb098c572dd2efe284f89084f46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5302.5751, "isoelectric_point": 4.285114479064942 }, "id": "GIHJHMOALN_386", "locus": "GCA009939745_00351" }, { "type": "cds", "sequence": "contig_1", "start": 198924, "stop": 200120, "strand": "+", "frame": 3, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P75034" ], "nt": "TTGCTTGACTTTTCTAATAGGGTAGCTAAGTCATTTAGCAATATAAACACTAACACCAATAAAAAGGGCACCGATATTCAAGGTGTTCTGGGAATCGATAGCACTCCTAACTCCTTATTTACCTCTGTTTTTGCTGCAGGTGGCGGTAACTATGACAACTTCTTTTATAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCTTACCAAAACTTACAAAAAGTTTATGGTGATTACAAAGGCTTGATTGACAGCAATGGTCTCTTTGTTAATAAGGGTGGTTCTTATTCATCGAACTTCCAAAAGTTTCACCAATTAGCTTACAGTATTTCCTCGACTTCCGGCTTCTTCTACTCGTTTGCTGGTGAAAACTCAAAGCGCTTGAAGTTTGATGGCGGTAATTTTATTGAGTTTCCTGGATTTACTCAAGCAATTTATGCGCCTGAAAATAATAGTCAAAGTGGTGGAGAGAGTGGCGATCAAACCACAAATAATGAAGCCAATCTTTTAGGCACTTTCAAAATAAAAAGCTCTAATAAATCTAAATCTTCAAGTTCCAAATCAAGCACTAAAGCCGAAACAGGCAAAACTAGTGGTGGAGACCAAAACCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAACGGAGGTGAAGAAACAACTATTTACCTTTATAAAAACTCAATTCCATCTGGAAAAAATAAAGGCGAAAATGCAATTCTTATAGATAACAAAACTATTGTGGAACAACTAGAAAGTGCGGCTAAAAAAGAAGATAAGAAGAGTGGCGAATCTACCACAGAACAGACACAAATACAAAGTAAAAGTGTAACAGAACAAAAACAACCCAAAATCATCGGTTACACTACCACTGGCAATGTTCACGAAGACAACAAACACATTTTCCCAATTGACAAACTTACATCTGATCGAAATTTTGACCGCAAAATTATTGTTGGTGCCACTGAAGAAACACTCGATAAATCGAATACTTTGCAGTCCAATGAAGCCATTGTTTTACCAGCACCCTCTAAGTACAAAAGTACTGACACTAACAAAGTAACCATCACCCAAGGTCCGAATATCATTGGAATTCACGCCAACGAAAAGGAAAATGCAGAAACACAAAAGTTTGTGGATTGA", "aa": "MLDFSNRVAKSFSNINTNTNKKGTDIQGVLGIDSTPNSLFTSVFAAGGGNYDNFFYKVANGRADFSNFKNKGTSYQNLQKVYGDYKGLIDSNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGENSKRLKFDGGNFIEFPGFTQAIYAPENNSQSGGESGDQTTNNEANLLGTFKIKSSNKSKSSSSKSSTKAETGKTSGGDQNQGKKGEGAQNQGKKGEGAQNQGKNGGEETTIYLYKNSIPSGKNKGENAILIDNKTIVEQLESAAKKEDKKSGESTTEQTQIQSKSVTEQKQPKIIGYTTTGNVHEDNKHIFPIDKLTSDRNFDRKIIVGATEETLDKSNTLQSNEAIVLPAPSKYKSTDTNKVTITQGPNIIGIHANEKENAETQKFVD", "aa_hexdigest": "0eab26311ca660acbccde69ba9c1b775", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 198624, "stop": 200423, "observations": { "mutation": [ 198687, 198915, 200118, 200142 ], "stop": [ 198687, 198915, 200118, 200142 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75034", "query_cov": 1.0, "subject_cov": 0.4830097087378641, "identity": 0.997, "score": 697.0, "evalue": 1.61e-245, "gene_start": 1, "gene_end": 398, "reference_start": 296, "reference_end": 693, "reference_sequence": "MKFKYGAIVFSCLLGVSAILAACGARGKFNQVDDGKIKLASSLTSRSASTALQKVVEKYNKVKGVNDYPIEITQIAGGYDGGRTDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELPFDKVKTDKLADRFLDFNNRISAISKKGIYGIPVSLSTEVLSINGPVLHYILNSAQGKVSSVTSVQRTAASGSGTTNNNGVTKPLKIDKENESTKKLWEEIENKAKENGKKTSSSRRKRNLSSSKQMSTQTQPTDNSNDANQSDQAIEKTWGKYQEVEGGLKDYTFKASVFENWHDLLDFSNRVAKSFSNINTNTNKKGTDIQGVLGIDSTPNSLFTSVFAAGGGNYDNFFYKVANGRADFSNFKNKGTSYQNLQKVYGDYKGLIDSNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGENSKRLKFDGGNFIEFPGFTQAIYAPENNSQSGGESGDQTTNNEANLLGTFKIKSSNKSKSSSSKSSTKAETGKTSGGDQNQGKKGEGAQNQGKKGEGAQNQGKNGGEETTIYLYKNSIPSGKNKGENAILIDNKTIVEQLESAAKKEDKKSGESTTEQTQIQSKSVTEQKQPKIIGYTTTGNVHEDNKHIFPIDKLTSDRNFDRKIIVGATEETLDKSNTLQSNEAIVLPAPSKYKSTDTNKVTITQGPNIIGIHANEKENAETQKFVDWFLNSPQTWEGKGKGKEQTNKTAAEFFAESASYILPLKEIFEQKNEKKEGSDQKDSKSNGRGKQTNLYTEKALELFRGISTDQIVSYSDPSDFRSGRFRDGIGATFNAAVSSKASFEKFVQNFIATLGSDI" }, "paralog": false, "identity": 0.993322203672788, "score": 1114.0, "evalue": 0.0, "description": "Internal stop codon at 198687, 198915, 200118, 200142. Nonsense mutation around 198687, 198915, 200118, 200142.", "psc": { "uniref90_id": "UniRef90_P75034", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75034", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "GIHJHMOALN_387", "locus": "GCA009939745_00352" }, { "type": "cds", "sequence": "contig_1", "start": 200220, "stop": 200513, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCACTCAAGGAAATTTTTGAGCAAAAGAATGAAAAGAAAGAAGGCAGCGATCAAAAAGATAGTAAAAGCAATGGTAGAGGTAAACAAACTAACCTCTACACCGAAAAGGCCTTGGAACTATTTAGAGGTATTAGTACCGATCAAATAGTAAGTTACAGTGACCCAAGTGACTTTAGGAGTGGTCGTTTCCGTGATGGAATAGGTGCCACTTTTAACGCAGCAGTAAGCTCTAAAGCATCTTTTGAAAAGTTTGTTCAAAACTTTATTGCAACTTTAGGTAGTGATATTTAG", "aa": "MPLKEIFEQKNEKKEGSDQKDSKSNGRGKQTNLYTEKALELFRGISTDQIVSYSDPSDFRSGRFRDGIGATFNAAVSSKASFEKFVQNFIATLGSDI", "aa_hexdigest": "7e6d7d2e5f9ef87dd24074610f5d1eac", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10805.850299999987, "isoelectric_point": 7.944713020324707 }, "id": "GIHJHMOALN_388", "locus": "GCA009939745_00353" }, { "type": "cds", "sequence": "contig_1", "start": 200834, "stop": 201052, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGCATTGAAGGTAATGTTTACAAACACTTCCAAACTGAACTTAGTCGCTTTTACAAGCAAACGCGCAAACACTTTCGCGAAACTGGTTCGGAAAGTCTCTTTTTAGGTTTACCAGTAATTGAGGGCATTAACGAGTTTAACGATGTCTTTCGTGCTCCTTTACTCTATGTGGGGGTTAAGTTAAAAGTAGCACCACGACTTGAACGCTTTTGA", "aa": "MFGIEGNVYKHFQTELSRFYKQTRKHFRETGSESLFLGLPVIEGINEFNDVFRAPLLYVGVKLKVAPRLERF", "aa_hexdigest": "d192c8883fe593f4effd274614fb1862", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8465.699599999993, "isoelectric_point": 9.518192863464353 }, "id": "GIHJHMOALN_389", "locus": "GCA009939745_00354" }, { "type": "cds", "sequence": "contig_1", "start": 201053, "stop": 201976, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGGAAATAAACCGCGAGGAAATCTTTTTAAATCCGACCATCATCGGGGTTGAAATTAACAAGCGTAACAGCTTGTTTAAAAATAACTATGACACCACTAAGGTTGATATTAACCAGGCATTGGAAATCTTTCGTGAGTTGGAATACCAATTTCGGATGCCACTCACCTCGGAGTTAAAAAGTTTTTCCAAAAAGGCTAAGAGTGACTTTAACACTGAAAAACGGACTAACTTCCTCACTAATAACGTTCTTTTAGGAATCTTTGACGTTAAGGGGGATCAACTGTTCCAAAACTTTAACGAAATTCTAAACACTGATCCTGATGTTTTGGATGAATTGTTAAAGGATCGCCGCGATCTCTTATACGACAACCGTGAGTTTCGTGAAAACTTTAACTTAAAGGGCACTTACCTCTTTAGCCACTTAGACATCTTCCAGCAGTACGCTGTCAAACAGGCATTTGATGGGGATGTCATTATTGAAGGTCCACCTGGTACTGGTAAGAGTGAAACGATTGTCAACATCTTGGTCAACCTCGCTTTAAACAAAAAGAAGGTGTTGTTTGTGTCAGAAAAGGTAACTGCCTTAGATGTCGTTTACAACCGGCTAGGTAGCTTTAAGCACATTGCCTTATTTAATGCTTCGGTAGCGAGTGAGAAGAAGCGCTTTTACAGCCAGTTCGCTGATTATGAAAGCTTCTTTACCGATAACTTTTCCAAGAAGGATTTAGTTAACGAAATGCCGGTGTTTGACGGGCAGTGGGTTGATAAGATCCTAAGTGAATTCACTAACCTTCAAAACATTTACGATACCCAAATTAATTCGGGTAACCAGAGCTATTCCTTCAAGGAAATCCTAAGTAGTTTTCCAATACTTGATGTCAGTTACATTAAGATCAAGGAACACGATCGTTTTGATGAATGA", "aa": "MEINREEIFLNPTIIGVEINKRNSLFKNNYDTTKVDINQALEIFRELEYQFRMPLTSELKSFSKKAKSDFNTEKRTNFLTNNVLLGIFDVKGDQLFQNFNEILNTDPDVLDELLKDRRDLLYDNREFRENFNLKGTYLFSHLDIFQQYAVKQAFDGDVIIEGPPGTGKSETIVNILVNLALNKKKVLFVSEKVTALDVVYNRLGSFKHIALFNASVASEKKRFYSQFADYESFFTDNFSKKDLVNEMPVFDGQWVDKILSEFTNLQNIYDTQINSGNQSYSFKEILSSFPILDVSYIKIKEHDRFDE", "aa_hexdigest": "aacb909093bef0fa201f8ade522348c4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 73, "aa_cov": 0.20846905537459284, "hmm_cov": 1.0, "evalue": 1.32066410614636e-09, "score": 33.647674560546875, "start": 142, "stop": 205 } ], "seq_stats": { "molecular_weight": 35840.027000000024, "isoelectric_point": 5.108881950378417 }, "id": "GIHJHMOALN_390", "locus": "GCA009939745_00355" }, { "type": "cds", "sequence": "contig_1", "start": 202118, "stop": 202351, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGTGCTATGTGTTGGATTACTTCGAGCAGAACAACAGCATAATCAAACCGAAGCGGGTATTGCTCTATACCCCCACTGAACGCGGGCAAAAGCAGTTGCACCAACTCCAGCAAGACGTTGCTAAGTACAACAGCTTGCAACGTTTTAAATCGGCTGCTAAGTTTGAAACAATTAAGCTCAATTTAGCTAACAAACTAGCACAAAACGCCAAGCCTTTTTTCTTTTCTTGA", "aa": "MLCYVLDYFEQNNSIIKPKRVLLYTPTERGQKQLHQLQQDVAKYNSLQRFKSAAKFETIKLNLANKLAQNAKPFFFS", "aa_hexdigest": "b38c3cda4b3cce2177ce1bfc3b606855", "start_type": "GTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9069.429899999994, "isoelectric_point": 9.812168693542482 }, "id": "GIHJHMOALN_391", "locus": "GCA009939745_00356" }, { "type": "cds", "sequence": "contig_1", "start": 202379, "stop": 203071, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGGAAAATCTCGTACAAACCCAAAAGCAATTGGTTAAGGCTAAGCAAAGCTACTTAAGCAAAATTGAGCAGTATGTAGTTAGTTGTAAACGGATTCTCAAAGCTACCATTTTAGCCAACTTCTTTGAGTTGTACCAAACCAATAAGAATGAACTATTGGATATCTGTCGGGAAGCCAAAAACCCTGTTTTAAAGGAAATTACCTGGTGGTTTAAGAAGAACTTTGCACTGTTATCAAAGCTATTCCCCGTCCACATTATGACGTTTGAATCAGCCGCCTTATTAACCCCGAACCAGCGCCGTTTGTATGACTATGTGGTGATTGATGAAGCTAGCCAGGTTTATCTAGAACGTGCCATTCCAATCTTATACCGTGGCGCTAAGTACATTATTGCTGGAGACACCAAACAATTAAAACCATCCAATTTCTTCCAAGCCCGGGCTGAGTATGATGTGGATGAAGAGTTTGAAGATGGTAACGTTGAAGCTGCGGTGCACTCGACTTCGTTACTACACTTTTTGAAGAACCGTTCGCGCATTTTAACGCTGCTCAAATTCCACTACCGCAGTGATTCCGCTAACCTAATTGCCTTTACTAATAATCGCATTTATAACAACGAGCTCATCTTTATGAATAAAGCAACCGCTGACAAGCAGGTCTTTATTGTCCATGATGTCATCGATGGTATTTGA", "aa": "MENLVQTQKQLVKAKQSYLSKIEQYVVSCKRILKATILANFFELYQTNKNELLDICREAKNPVLKEITWWFKKNFALLSKLFPVHIMTFESAALLTPNQRRLYDYVVIDEASQVYLERAIPILYRGAKYIIAGDTKQLKPSNFFQARAEYDVDEEFEDGNVEAAVHSTSLLHFLKNRSRILTLLKFHYRSDSANLIAFTNNRIYNNELIFMNKATADKQVFIVHDVIDGI", "aa_hexdigest": "d305f28155a62318e0b718863ee1659c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 42, "aa_cov": 0.1782608695652174, "hmm_cov": 1.0, "evalue": 1.2470017357622415e-09, "score": 33.72935485839844, "start": 100, "stop": 140 } ], "seq_stats": { "molecular_weight": 26809.62560000002, "isoelectric_point": 8.885886955261231 }, "id": "GIHJHMOALN_392", "locus": "GCA009939745_00357" }, { "type": "cds", "sequence": "contig_1", "start": 203252, "stop": 203863, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "GTGAACGCTCAGGGCGAGTATGTCGGTTTGTTTGTCAAAAACATTGAAAACGTTCAGGGTGATGAACGGGACATTATTATCTTCTCGTTGGGCTATGACCGTTCTGTCAACAGCTATGGCCCGATTTCCAAGCAAGGTGGGGAGAACCGTTTGAATGTAGCGATTACCCGTGCTAAGCAGCGGATTGAACTGTTCAAGACTAACCGTGCTAGTGATTACAATGGTTTGAGCTCCAATTCCTTGGGTAGTAAGTTATTGGTGGAATACTTACTGTACTGTGAGGCAATGGCCAACAACCAGGGTGAGAGCTTAGACTTCCAAGCAACCCAAAAACAAGCACCCAAGGCCAAGTACGAATTGGAATTGGAAAACCAGTTCTTTAATGAGTTGGAACTTATTTTTGGTGAGCAGTTTACGATTAAACGCAATGTCAATGAGGGTGCATACAGCTTTAGCTTTGTCTTTTACTTTAACGAAAACCCTTACCTAGCGGTTGACTTTAACCCTGCTTTACCCCACTCCAGAAAGGAAGTGAGTGAAAACATTATTTACCGCGAACAATTTTTGAAAAAGCGGAAATGGAACCTCGTTAACATTTGGTTAGACGAGTGA", "aa": "MNAQGEYVGLFVKNIENVQGDERDIIIFSLGYDRSVNSYGPISKQGGENRLNVAITRAKQRIELFKTNRASDYNGLSSNSLGSKLLVEYLLYCEAMANNQGESLDFQATQKQAPKAKYELELENQFFNELELIFGEQFTIKRNVNEGAYSFSFVFYFNENPYLAVDFNPALPHSRKEVSENIIYREQFLKKRKWNLVNIWLDE", "aa_hexdigest": "542c68ba3f61a75ff8891bc9036adde9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 53, "aa_cov": 0.26108374384236455, "hmm_cov": 0.9433962264150944, "evalue": 1.0559912783388579e-09, "score": 33.67409133911133, "start": 10, "stop": 62 } ], "seq_stats": { "molecular_weight": 23528.12060000002, "isoelectric_point": 5.281274604797362 }, "id": "GIHJHMOALN_393", "locus": "GCA009939745_00358" }, { "type": "cds", "sequence": "contig_1", "start": 204018, "stop": 204638, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "ATGAACAATCAATCAAATCACTTTACCAATCCTTTGTTACAACTGATTAAGAACGTTGCCGAGACCAAGAATTTAGCCATCGATGATGTTGTTTTGTGTCTTAAAACAGCATTAGAACAAACATACAAGAAGCATTTGAACTATGTCAATGTGGAAGTCAACATTGACTTTAACAAGGGCTTAATGCAAATTGAACAGCTCTTCGATGTTGTAGATGATAACAATGAGGATTATGATGACTTTTTGGAAATGCCTTTAAGTGAGGCTAAGAAGCTCAATCCGAACCTAGAAGTTGGCGGGGTGTTGCGCAAACCGGTTTCACTCAAAGACATTAAGGGCGATCTCATTAGCAAAATGGTATTACTCTTCAACCAAAAGATTAACGAAACAGCTTTTAAAACGGTGATGAGTGACTTCATTAATGAAGTCGGTCAGGTAATTGAAGCTAGAGTAGAGGACATTGATACGAACAAAGATGGTGGATTGAAGGGTTACATTGTTAACTTGGAAACCACCAAGGGTTACATGCCTAAACGTGAGTTGAGTAAGGGCGAAAAGCTAGATATTGGCAAGAAGTACCTCTTTGTGATTAAGGAAATCCAAAAGCAATCTTCGATGTGA", "aa": "MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALEQTYKKHLNYVNVEVNIDFNKGLMQIEQLFDVVDDNNEDYDDFLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMVLLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDGGLKGYIVNLETTKGYMPKRELSKGEKLDIGKKYLFVIKEIQKQSSM", "aa_hexdigest": "4064af306544f97da505e54264cad8e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 120, "aa_cov": 0.5776699029126213, "hmm_cov": 0.9583333333333334, "evalue": 7.086953808034977e-20, "score": 66.93895721435547, "start": 12, "stop": 130 } ], "seq_stats": { "molecular_weight": 23473.773300000008, "isoelectric_point": 5.225913429260252 }, "id": "GIHJHMOALN_394", "locus": "GCA009939745_00359" }, { "type": "cds", "sequence": "contig_1", "start": 204681, "stop": 204887, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13184.12" ], "nt": "TTGCTAAATTCCAACACTCCCGAAATTGCTAACGGTACGATCGAAATTAAAAAGATGGAACGCTCTCCTGGTACCAAGTCTAAGGTGGCCGTGATTTCCAAAGACCCAGTAGTTGATCCGATTGCGGCCATTTTGGGTCCCAAGGGCGAGCGCATTCGCGGCATTAGTGAAGAGTTTAACGGTGAGATCATTGATATTGTCATCTGA", "aa": "MLNSNTPEIANGTIEIKKMERSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVI", "aa_hexdigest": "3cc5c7e3d8110d3a85095e519a519cb2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13184.12", "name": "KH_5", "length": 45, "aa_cov": 0.6617647058823529, "hmm_cov": 1.0, "evalue": 2.1117877776959867e-13, "score": 45.73484802246094, "start": 23, "stop": 67 } ], "seq_stats": { "molecular_weight": 7301.397599999999, "isoelectric_point": 5.311058235168456 }, "id": "GIHJHMOALN_395", "locus": "GCA009939745_00360" }, { "type": "cds", "sequence": "contig_1", "start": 204909, "stop": 205640, "strand": "+", "frame": 3, "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "db_xrefs": [ "GO:0003700", "GO:0003723", "GO:0005829", "GO:0006353", "GO:0031564", "SO:0001217", "UniRef:UniRef50_P47387", "UniRef:UniRef90_P75591" ], "nt": "TTGGTCAACGCTGTTTTACCGGCTGAAGTGGTCGGTTACAACATCCTTCAAGACGATGAGCGCGATACCAGCATTGAAATCGTGGTACCCGCCAACCAGATTGCCAACGTCTTTGGTTTTAAAGGAATTAACATTCGCTTAATTAGTAACCTAACGGGTTGGAGTAGTGTGGACGTTTACACCGAAAAAGATGCCGCTGAACAAGGGATTGAATACACCCGGGTCAACTTTCAACCCCAAGGTATTTTTGGCATCAAAAAGCGCCGCGACAAGATCTCCAACAATCCACGCAACAACAACCAACAGCTCGCATCTGATAAGGTGTTTTACACTTCGAAGGCCAATGTGGTTGATGATGAGATCATAGTCGATCTAGCAAAGCAAGCGGAAGCCAAACGGGTCAAGCAAATCAAGCAGGAAGCAACTAAACCCGAGTTGCAATTACAACAAGAACTTAACCTCGAAGCAACCCCTAAGGTAGCTGCTCCAACCCCAACCCCTGCACCCCAACCAACTCCCGCACCAACTAAAGTTGAGCCAGTACCACCACCAGTAAGCGTTACACCTAAGCCAATCCCCAAGGTCAATAAGCCCAAGCCAGTGGTTAAACCAAAATCAGTCTTTTCGATTACGGTTGAAGCGGATGACAGTAAGACTAAACCAGAAAAGTCAAGCGCTAAAACTAACACCCCACAAACGAAACAAACCTTTGATAACTTTGACGATCTCTAG", "aa": "MVNAVLPAEVVGYNILQDDERDTSIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDAAEQGIEYTRVNFQPQGIFGIKKRRDKISNNPRNNNQQLASDKVFYTSKANVVDDEIIVDLAKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPPPVSVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL", "aa_hexdigest": "2160ce94dd7a92c2d39f420208ca3dfd", "start_type": "TTG", "rbs_motif": null, "genes": [ "nusA" ], "truncated": "5-prime", "pseudogene": { "start": 204609, "stop": 205640, "observations": { "mutation": [ 204636, 204885 ], "stop": [ 204636, 204885 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75591", "query_cov": 1.0, "subject_cov": 0.45, "identity": 0.992, "score": 374.0, "evalue": 5.66e-125, "gene_start": 1, "gene_end": 243, "reference_start": 298, "reference_end": 540, "reference_sequence": "MNNQSNHFTNPLLQLIKNVAETKNLAIDDVVLCLKTALAQTYKKHLNYVNVEVNIDFNKGLMQIEQLFDVVDDNNEDYDDFLEMPLSEAKKLNPNLEVGGVLRKPVSLKDIKGDLISKMVLLFNQKINETAFKTVMSDFINEVGQVIEARVEDIDTNKDGGLKGYIVNLETTKGYMPKRELSKGEKLDIGKKYLFVIKEIQKQSSMWPITLSRSDSRLLEFLLNSNTPEIANGTIEIKKMERSPGTKSKVAVISKDPVVDPIAAILGPKGERIRGISEEFNGEIIDIVIWNEDKLKFLVNAVLPAEVVGYNILQDDERDTSIEIVVPANQIANVFGFKGINIRLISNLTGWSSVDVYTEKDAAEQGIEFTRVNFQPQGIFGIKKRRDKISNNPRNNNQQLASDKVFYTSKANVVDDEIIVDLAKQAEAKRVKQIKQEATKPELQLQQELNLEATPKVAAPTPTPAPQPTPAPTKVEPVPPPVSVTPKPIPKVNKPKPVVKPKSVFSITVEADDSKTKPEKSSAKTNTPQTKQTFDNFDDL" }, "paralog": false, "identity": 0.9912536443148688, "score": 576.0, "evalue": 9.56e-207, "description": "Internal stop codon at 204636, 204885. Nonsense mutation around 204636, 204885.", "psc": { "uniref90_id": "UniRef90_P75591", "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "uniref50_id": "UniRef50_P47387", "go_ids": [ "GO:0005829", "GO:0003700", "GO:0003723", "GO:0006353", "GO:0031564" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75591", "UniRef:UniRef50_P47387", "GO:0005829", "GO:0003700", "GO:0003723", "GO:0006353", "GO:0031564" ] }, "pscc": { "uniref50_id": "UniRef50_P47387", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47387" ], "product": "Transcription termination/antitermination protein NusA" } }, "id": "GIHJHMOALN_396", "locus": "GCA009939745_00361" }, { "type": "cds", "sequence": "contig_1", "start": 205665, "stop": 205934, "strand": "+", "frame": 3, "gene": "ylxR", "product": "Transcription termination protein", "db_xrefs": [ "COG:AJ", "COG:COG2740", "KEGG:K07742", "RefSeq:WP_015344887.1", "SO:0001217", "UniParc:UPI0002B84478", "UniRef:UniRef100_A0AB38W8E2", "UniRef:UniRef50_A0AB38W8E2", "UniRef:UniRef90_A0AB38W8E2" ], "nt": "ATGCAAAGTTGGCGCCAATGTTTAGTTACCAGAAAGTCTTTTGCTAAGCCGCTACTTTTGCGTCTTGTAAAATTAGAGCATCAACTACGGGTGGATTTAGCGCAAACACTACCAGGTCGGGGGTATTATTTAAGTGTAGCTGCTTTAAAACTGGAGCGGAAAAACTTTAAAGCACTGTTACAAAAACGCTTAAAGGTAAGCTGTTCTGACGCAGAGCTAGATCACATTATTGCTTGTTTAAAGGAAAGGGAAAGCGATGTCAAAGCATAA", "aa": "MQSWRQCLVTRKSFAKPLLLRLVKLEHQLRVDLAQTLPGRGYYLSVAALKLERKNFKALLQKRLKVSCSDAELDHIIACLKERESDVKA", "aa_hexdigest": "4776d9d969246ce69f2cdc0f210ccdfa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B84478", "ncbi_nrp_id": "WP_015344887.1", "uniref100_id": "UniRef100_A0AB38W8E2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B84478", "RefSeq:WP_015344887.1", "UniRef:UniRef100_A0AB38W8E2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8E2", "uniref90_id": "UniRef90_A0AB38W8E2" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8E2", "gene": "YlxR", "product": "Transcription termination protein", "uniref50_id": "UniRef50_A0AB38W8E2", "cog_id": "COG2740", "cog_category": "AJ", "kegg_orthology_id": "K07742" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8E2" ], "product": "Transcription termination protein" }, "genes": [ "ylxR" ], "id": "GIHJHMOALN_397", "locus": "GCA009939745_00362" }, { "type": "cds", "sequence": "contig_1", "start": 205921, "stop": 207774, "strand": "+", "frame": 1, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413", "KEGG:K02519", "RefSeq:WP_014325382.1", "SO:0001217", "UniParc:UPI0001DD64D7", "UniRef:UniRef100_A0AAX0SR00", "UniRef:UniRef50_P47388", "UniRef:UniRef90_P75590" ], "nt": "ATGTCAAAGCATAAACCACGTCATTTTCAAAAGAATAAGTTTGACAACCGGGCTAAAACGAGTGCCAAACAACAGTTTCGCCAGGTAAAAACCGGTGTTAAAGATGGTGTCTTTGTCTATAAGGGACCACTCACTGTTAGTGAGTTTTGTCTCAAAACCAATATTCCGACGGCCAACATCATCAAGCACTTCTTTTTAAATGGGGTTCCCTTAACCTTAAATTCGGTGTTATCGACCGAACAACTAGCTGACGCTTGTGTTAACTTTGGCTTTGACTTTAAGGTAGAAACCGAAATCACCCACGACAACATTATCTCCAACATTAAGTTTGACGATGATCCCACCCAGTTAAGTCCAAGACCCCCGATCGTTACCATTATGGGTCACGTGGATCACGGGAAAACATCCTTGTTAGACGCCATTCGCCAAACTAATACAGCAGCCAAAGAGTTTGGTGGCATTACCCAAAAGATTGGGGCGTACCAGGTCAAAAACCAGGAGGGCAAAACGATTACCTTCATTGATACCCCCGGTCATGAAGCCTTTACCGGCATGCGCGCCCGTGGTGCTCAAGTGACTGACATTGTTGTCTTAGTGGTAGCGGGTGATGATGGCTTAAAACAACAAACTGAGGAAGCGATTTCCCACGCCAAGAGTGCCAAAACCCCGATCATTGTCTTTATTAACAAGATGGATAAACCAACCGCTAACCCCGACATGGTGATCCAACAGCTCAACAAGTTTGATTTAGTCCCTGAAGAATGGGGTGGTGATACCATCTTTGTCAAGGGCAGTGCCTTAACCAAAGAGGGGATCCAGGAGCTCTTGGACAGTATTCTGTTAGTAGCGGAAGTGGAAGATTACAAGGCCAACTTTAACGCCCACTCATCGGGTTATGCGATTGAGGTGCAAACCACCAAGGGGTTGGGTCCCACCGCTACCATTATTGTTAAACGTGGTACCCTAAAGATTGGTGACATCGTTGTCTTGGGTCCCGCTTGGGGTAAGGTTCGTACCATGCAAGACGAAAACGGAGTCCATCTCCAAGAAGCTATGCCCTCTAAACCAGTACAAATTTCGGGGTTTGACATTGTCCCAGTTGCTGGGGAAAAATTCATTGTCTTTGACGATGAAAAGGATGCGAAGTTAATTGCCAATAAGTTCCGCGAACAGCAAAAACAAAAGCTCAATACCACCCAAATTAACGAGGTGCTCAAACAAAAGATTAAGAGCAAGGAAATTAAGGTTTTAAATCTGATTTTTAAGGTTGACAGTGATGGTAGTTTAGCGGCCATTAAACAAGCTATGCAGAGCATTGATGTGCCGGGGATGAGCGTCAACATTATCCACTCAGGTGTTGGTTTAATCTCGGAAAACGACATTATGTTAGCCAAGGCGTCCGGGGCATTACTGTTTAGCTTGAACCTCGGTTTAAGTCAAGTTGTTAAGAACATTGCTAGTCTTCAAGGAGTGAAGGTCGATGTCCACTACCACATCCCTAAGTTAGCGGAAGAGATCGAAAACATCTTAAAGGGCCAGTTAGAACCAGTGTACGAAGACGTGGAACTGGGGCGTGCTGAAGTGTTACAACTCTGGTACCACTCCAAGGTGGGGCACATTGCTGGTACCTTAGTTAAAACAGGTAAGGTTAAACGGGGCGCCTTGTGTAAGCTGTTACGCCGTAACGAAACCATCTATGAAGGCCGGGTGGACTCATTGAAGAGTGAAAAGAACCCGGTCAACCAGATGGAAGCGGGGAAGAACTGCGGTATTGTTATTAATGGGTGTGAGGACATTCAGGTGTGCGACATTATCTTGGTGTACGAAAAGCAGGAGGTCAAGTCCAAAAGTTAA", "aa": "MSKHKPRHFQKNKFDNRAKTSAKQQFRQVKTGVKDGVFVYKGPLTVSEFCLKTNIPTANIIKHFFLNGVPLTLNSVLSTEQLADACVNFGFDFKVETEITHDNIISNIKFDDDPTQLSPRPPIVTIMGHVDHGKTSLLDAIRQTNTAAKEFGGITQKIGAYQVKNQEGKTITFIDTPGHEAFTGMRARGAQVTDIVVLVVAGDDGLKQQTEEAISHAKSAKTPIIVFINKMDKPTANPDMVIQQLNKFDLVPEEWGGDTIFVKGSALTKEGIQELLDSILLVAEVEDYKANFNAHSSGYAIEVQTTKGLGPTATIIVKRGTLKIGDIVVLGPAWGKVRTMQDENGVHLQEAMPSKPVQISGFDIVPVAGEKFIVFDDEKDAKLIANKFREQQKQKLNTTQINEVLKQKIKSKEIKVLNLIFKVDSDGSLAAIKQAMQSIDVPGMSVNIIHSGVGLISENDIMLAKASGALLFSLNLGLSQVVKNIASLQGVKVDVHYHIPKLAEEIENILKGQLEPVYEDVELGRAEVLQLWYHSKVGHIAGTLVKTGKVKRGALCKLLRRNETIYEGRVDSLKSEKNPVNQMEAGKNCGIVINGCEDIQVCDIILVYEKQEVKSKS", "aa_hexdigest": "91d8a369556c2040bd580e7b0a30a727", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64D7", "ncbi_nrp_id": "WP_014325382.1", "uniref100_id": "UniRef100_A0AAX0SR00", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64D7", "RefSeq:WP_014325382.1", "UniRef:UniRef100_A0AAX0SR00" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SR00", "uniref90_id": "UniRef90_P75590" }, "psc": { "uniref90_id": "UniRef90_P75590", "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_P47388", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_P47388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47388" ], "product": "Translation initiation factor IF-2" }, "genes": [ "infB" ], "id": "GIHJHMOALN_398", "locus": "GCA009939745_00363" }, { "type": "cds", "sequence": "contig_1", "start": 207777, "stop": 208127, "strand": "+", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024", "KEGG:K02834", "RefSeq:WP_010874513.1", "SO:0001217", "UniParc:UPI00001331B1", "UniRef:UniRef100_P75589", "UniRef:UniRef50_P47389", "UniRef:UniRef90_P75589" ], "nt": "ATGGCTAGTTACAAAAAGGAACGTCTGGAAAACGACATTATTCGTTTAATTAACCGCACTGTTATTCACGAAATCTACAACGAAACGGTCAAAACCGGTCATGTAACCCACGTGAAACTATCGGATGATTTACTCCATGTAACAGTTTACCTAGACTGTTATAACCGTGAGCAAATTGACCGCGTGGTGGGTGCCTTTAACCAGGCCAAAGGGGTGTTTAGCCGCGTGTTAGCACACAATTTGTACTTAGCCAAAGCCGTGCAAATCCATTTTGTCAAGGACAAAGCAATTGATAACGCAATGCGCATTGAGTCTATCATCAACTCCCTTAAAAAATCAAAGCCTAATTAA", "aa": "MASYKKERLENDIIRLINRTVIHEIYNETVKTGHVTHVKLSDDLLHVTVYLDCYNREQIDRVVGAFNQAKGVFSRVLAHNLYLAKAVQIHFVKDKAIDNAMRIESIINSLKKSKPN", "aa_hexdigest": "ea805a36f7a0eafb24a3f0e2c3411375", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001331B1", "ncbi_nrp_id": "WP_010874513.1", "uniref100_id": "UniRef100_P75589", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001331B1", "RefSeq:WP_010874513.1", "UniRef:UniRef100_P75589" ] }, "ips": { "uniref100_id": "UniRef100_P75589", "uniref90_id": "UniRef90_P75589" }, "psc": { "uniref90_id": "UniRef90_P75589", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_P47389", "cog_id": "COG0858", "cog_category": "J", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0005829", "GO:0006364", "GO:0030490", "GO:0043024" ] }, "pscc": { "uniref50_id": "UniRef50_P47389", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47389" ], "product": "Ribosome-binding factor A" }, "genes": [ "rbfA" ], "id": "GIHJHMOALN_399", "locus": "GCA009939745_00364" }, { "type": "cds", "sequence": "contig_1", "start": 208143, "stop": 208988, "strand": "+", "frame": 3, "gene": null, "product": "putative MG144-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75588", "UniRef:UniRef90_P75588" ], "nt": "ATGAAACCACCTAAAAAGCCAGTCAACCCACCGTTTGGTAAGCTAACTGATTCTAAAGCTGGAGTAGTCAAAGCTACCTCTAGTCAAGAGACCAAAAAAGTAGCTGACACCAAACCCAAAAATAAGGGTGGTTTGTTTAGCTTCTTTAAAAAAGACAAAACGGAAAAACCAGCTAAAGCAGCCAAACCCAAAGATGCCTTTAAGAGTGCGATAGCTGAATTGAATCCGAAAGCCAATCCTAAGACAGTCAAAGCGGATGTTGCACCGGCTAAAATTCCCCACAGCGATAAGGGTACGGTTACCCCTGTAGAGTTAAAACCCCAAACGGAAGCACCGTTACCACCAGGAGTTAAACAACCTGATCCTAAAAAAGATAAACCTAAGGGCGGTTTATTTGGCTTCTTCAAAAAAGATAAGAACAAAGATGTCAAAAAGGAGCCTGCTAAGCCAGCTACTCCAGTAAAAACTGAACCAACCTCACCTAAGGTAGAACCAACTAAGGTCAAACCACCGGGCGGTGTACCGACTAAGCCGGTAGTGGAAAAACCAGTGTCAGCTCAACCAACGGTACCATTACAACCAGAACCTACTTTCCCAGTCAAAGCAGCACCACTACCACCAGGTGTAAAAGCAGAACCGGAAAGCAAAAAACGCTTTGGTTTGTTTAAGGCATTCCAGAAAGATGACGCTAAACAACCCAAGCAAAAGCTCAATCTCCAAGACCAACAGGATCGCTTTATTGATGACCCCACCGCCAAAAAGCACTTTAGCGCCTTTAACCAAAAAGTTGGTAACTTACTGAAGGACAAAAAGACCCGTAACCGTGATTGGAAGATTGTGGGTTGA", "aa": "MKPPKKPVNPPFGKLTDSKAGVVKATSSQETKKVADTKPKNKGGLFSFFKKDKTEKPAKAAKPKDAFKSAIAELNPKANPKTVKADVAPAKIPHSDKGTVTPVELKPQTEAPLPPGVKQPDPKKDKPKGGLFGFFKKDKNKDVKKEPAKPATPVKTEPTSPKVEPTKVKPPGGVPTKPVVEKPVSAQPTVPLQPEPTFPVKAAPLPPGVKAEPESKKRFGLFKAFQKDDAKQPKQKLNLQDQQDRFIDDPTAKKHFSAFNQKVGNLLKDKKTRNRDWKIVG", "aa_hexdigest": "92342a83eaeca0e6f20fa17fcc07da2c", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 208143, "stop": 209291, "observations": { "mutation": [ 208986 ], "stop": [ 208986 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75588", "query_cov": 1.0, "subject_cov": 0.6990049751243781, "identity": 1.0, "score": 407.0, "evalue": 2.77e-139, "gene_start": 1, "gene_end": 281, "reference_start": 1, "reference_end": 281, "reference_sequence": "MKPPKKPVNPPFGKLTDSKAGVVKATSSQETKKVADTKPKNKGGLFSFFKKDKTEKPAKAAKPKDAFKSAIAELNPKANPKTVKADVAPAKIPHSDKGTVTPVELKPQTEAPLPPGVKQPDPKKDKPKGGLFGFFKKDKNKDVKKEPAKPATPVKTEPTSPKVEPTKVKPPGGVPTKPVVEKPVSAQPTVPLQPEPTFPVKAAPLPPGVKAEPESKKRFGLFKAFQKDDAKQPKQKLNLQDQQDRFIDDPTAKKHFSAFNQKVGNLLKDKKTRNRDWKIVGWIHGLILLFFIPLLAIMNKFVTLPAQSYPAVSLQVSINNALWGIAIFVIANIALPFITMFVLFLTGVRDVHASRPVHYFIWVLMLLNLTFLVISCCLLAAAYANLDLYNIWRNLQALDPNN" }, "paralog": false, "identity": 0.9973821989528796, "score": 625.0, "evalue": 1.03e-227, "description": "Internal stop codon at 208986. Nonsense mutation around 208986.", "psc": { "uniref90_id": "UniRef90_P75588", "product": "Uncharacterized protein MG144 homolog", "uniref50_id": "UniRef50_P75588", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75588", "UniRef:UniRef50_P75588", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75588", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75588" ], "product": "Uncharacterized protein MG144 homolog" } }, "id": "GIHJHMOALN_400", "locus": "GCA009939745_00365" }, { "type": "cds", "sequence": "contig_1", "start": 209034, "stop": 209318, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTGTGACCCTCCCAGCACAAAGCTATCCTGCTGTATCACTACAAGTATCGATTAACAATGCGTTGTGGGGGATTGCGATCTTTGTGATAGCCAACATTGCTCTACCCTTCATTACGATGTTCGTGCTCTTCTTGACTGGGGTCAGAGATGTGCATGCTAGTCGGCCAGTGCACTATTTCATCTGGGTGTTAATGTTGTTAAACCTTACCTTCTTAGTAATTAGTTGTTGTTTACTGGCTGCTGCTTATGCTAACTTAGACCTTTACAACATCTGA", "aa": "MNKFVTLPAQSYPAVSLQVSINNALWGIAIFVIANIALPFITMFVLFLTGVRDVHASRPVHYFIWVLMLLNLTFLVISCCLLAAAYANLDLYNI", "aa_hexdigest": "8abd6db1127ed8df2c152ac3a05b85c9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10504.50679999999, "isoelectric_point": 7.825124168395997 }, "id": "GIHJHMOALN_401", "locus": "GCA009939745_00366" }, { "type": "cds", "sequence": "contig_1", "start": 209364, "stop": 209867, "strand": "+", "frame": 3, "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "db_xrefs": [ "COG:COG0196", "COG:H", "EC:2.7.1.26", "EC:2.7.7.2", "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0008531", "GO:0009231", "GO:0009398", "KEGG:K11753", "SO:0001217", "UniRef:UniRef50_P75587", "UniRef:UniRef90_P75587" ], "nt": "ATGCAGCAAACCTTGATCATCGGTGCTTTTGATGGTTTACACAAGGGTCATCAATTGTTAGCACAAGCGGCTAGTGGTCCAGTGGTGGCCTTGTTAATTGCTAACATTCCCAGTCTACAAAGTGACTGGCTATACGAACCGAAGCAACGGCAAGTGCAGTTACAACAACACTTTCAGTCCGTGGTGCACAGCTATGATGTGATTGAACACAACATTAGTGCCCAAGCGTTTTTCGATCAAATCATTAGCCCACTACGGTGTCAGCAATTAGTCGTGGGTGCGGACTTTTGCTTTGGTAAAGACAACCAGAATGCTGATTTTTTAAGACGTTTATTTCCCAATACCACGATTATTCCCAAAGACAGTCAAACGCTGTCCAGTTCTACCGTTCGCCAATGGTTAAAGCAGGGCCAGATAGAACAAGCCAACGCCGTGTTATTGGAACCCTACTTTAGGGAAGGGGTGGTTATTCGTGGTAACCAGCAAGCCCGGTTTTTGGGCTGA", "aa": "MQQTLIIGAFDGLHKGHQLLAQAASGPVVALLIANIPSLQSDWLYEPKQRQVQLQQHFQSVVHSYDVIEHNISAQAFFDQIISPLRCQQLVVGADFCFGKDNQNADFLRRLFPNTTIIPKDSQTLSSSTVRQWLKQGQIEQANAVLLEPYFREGVVIRGNQQARFLG", "aa_hexdigest": "e278a7cdaa4c00ab56a988266a69e4a2", "start_type": "ATG", "rbs_motif": null, "genes": [ "ribF" ], "truncated": "3-prime", "pseudogene": { "start": 209364, "stop": 210170, "observations": { "mutation": [ 209865 ], "stop": [ 209865 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75587", "query_cov": 1.0, "subject_cov": 0.620817843866171, "identity": 0.9940000000000001, "score": 332.0, "evalue": 9.76e-114, "gene_start": 1, "gene_end": 167, "reference_start": 1, "reference_end": 167, "reference_sequence": "MQQTLIIGAFDGLHKGHQLLAQAASGPVVALLIANIPSLQSDWLYEPKQRQVQLQQHFQSVVHSYDVIEHNISAQAFFDQIISPLRCQQLVVGADFCFGKDNQNADFLRRLFPNTTIIPKDSQTLSSSTIRQWLKQGQIEQANAVLLEPYFREGVVIRGNQQARFLGWPTANITLKPYMVPLRCGSYVITVTYHQTNYPGVGFISYKNDQLVCETHLIGFSGDLYGKQLRFTFNQFIRPQQKFSGVQALQKAISGDLKQAQKWFAQSTN" }, "paralog": false, "identity": 0.9925373134328358, "score": 538.0, "evalue": 1.67e-197, "description": "Internal stop codon at 209865. Nonsense mutation around 209865.", "psc": { "uniref90_id": "UniRef90_P75587", "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_P75587", "cog_id": "COG0196", "cog_category": "H", "kegg_orthology_id": "K11753", "go_ids": [ "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0008531", "GO:0009231", "GO:0009398" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75587", "EC:2.7.1.26", "EC:2.7.7.2", "UniRef:UniRef50_P75587", "COG:COG0196", "COG:H", "KEGG:K11753", "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0008531", "GO:0009231", "GO:0009398" ] }, "pscc": { "uniref50_id": "UniRef50_P75587", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75587" ], "product": "Bifunctional riboflavin kinase/FMN adenylyltransferase" } }, "id": "GIHJHMOALN_402", "locus": "GCA009939745_00367" }, { "type": "cds", "sequence": "contig_1", "start": 209898, "stop": 210173, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01687.23" ], "nt": "ATGGTGCCGTTGCGCTGTGGGAGCTATGTGATTACGGTGACTTACCACCAAACCAATTACCCGGGTGTAGGGTTTATCAGTTACAAAAACGACCAGTTAGTCTGTGAAACCCACTTAATTGGTTTTAGTGGTGATCTTTACGGTAAACAACTACGCTTTACCTTCAACCAGTTTATTCGCCCCCAGCAAAAGTTTAGTGGGGTGCAAGCGTTGCAAAAAGCGATTAGTGGTGATTTAAAGCAAGCGCAGAAGTGGTTTGCTCAATCAACAAATTAA", "aa": "MVPLRCGSYVITVTYHQTNYPGVGFISYKNDQLVCETHLIGFSGDLYGKQLRFTFNQFIRPQQKFSGVQALQKAISGDLKQAQKWFAQSTN", "aa_hexdigest": "39d300f6a3c9eeaae46e8384c823eb85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01687.23", "name": "Flavokinase", "length": 87, "aa_cov": 0.9010989010989011, "hmm_cov": 1.0, "evalue": 1.137954484724492e-19, "score": 66.33644104003906, "start": 5, "stop": 86 } ], "seq_stats": { "molecular_weight": 10353.698899999992, "isoelectric_point": 9.469003486633301 }, "id": "GIHJHMOALN_403", "locus": "GCA009939745_00368" }, { "type": "cds", "sequence": "contig_1", "start": 210199, "stop": 210648, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGGAAAGTGCCCCCAGTGGTGGCCTGTTGGCCTTAATTATTATCAGTATTATCCTCCTGGCTTGTATTAGTGCCGTGGTTTCGGCTTACGAAACCGCCATTACCTCAATTACATCATACAAGTGGAGCAACTATGTCAAAACGCATAACAAGCAAAAGAAGTTAACGACCAAGATTGTTAACCACTTCCAAAAGAACTATTCGGCTTGTTTAATTACCATCTTAGTGGCCAATAACATTGTCGCCATTTTGGTTTCCAACATCCTCTTTTTGGCTTTAGATCAATCGATTAAGAATCCGGCCATTTCCAGTGCCTTGAACCTCTTAATTAGTGGGGTGTTATTGTTAATGCTGTGTGAAATTACTCCCAAAACCTTAGCACGGATTAACATCATCCGCGTCTTGGTCTACTTTGCGGTGGTAGTGTATTTTTTCTACATCCTTTTTTGA", "aa": "MESAPSGGLLALIIISIILLACISAVVSAYETAITSITSYKWSNYVKTHNKQKKLTTKIVNHFQKNYSACLITILVANNIVAILVSNILFLALDQSIKNPAISSALNLLISGVLLLMLCEITPKTLARINIIRVLVYFAVVVYFFYILF", "aa_hexdigest": "ad7724b1de6b65499b3fe5f323baff98", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 135, "aa_cov": 0.9060402684563759, "hmm_cov": 0.9629629629629629, "evalue": 1.5331815643332482e-20, "score": 69.0667495727539, "start": 15, "stop": 149 } ], "seq_stats": { "molecular_weight": 16423.589599999985, "isoelectric_point": 9.484218025207518 }, "id": "GIHJHMOALN_404", "locus": "GCA009939745_00369" }, { "type": "cds", "sequence": "contig_1", "start": 210844, "stop": 211473, "strand": "+", "frame": 1, "gene": "mpfA", "product": "UPF0053 MG146-like protein", "db_xrefs": [ "COG:COG1253", "COG:PT", "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q49399", "UniRef:UniRef90_P75586" ], "nt": "GTGGTGTACTGTTATGAAGGTGATCCAGTCAAGACCATTAAGGAAAAGTTCTTGCACGGCCAGTTTTCCAGAATGCCCGTACTCGACCAGACAAGCAATGAAGTGGTTGGTTTTATCCACCTCAAGGACTTGTTTAGTTCACTCGAAAAGAGCAACGAGCCGTTTGTTTTACAGGAGTTATTGTATCCAGCTGTTTTAGTAAGTAACACCACACCCATCAAGCAAGCTTTGCGCCAAATGCGGTTACACCGGGCCCACTTAGCGGTCGTGCAGGACAAACACCACCACACGATCGGGATTGTTTCTATGGAAGACATCATTGAGGAATTAGTCGGGGAAATTTACGATGAACATGATGAGGTGGAGGCAGTGCAAACGTTGGACAACAACACCTGGTTAGTGTTACCGAACGTAAAAGCCGCCTTTTTCTTTAACAAGTGGATTAAACGGGATTTAGTAAAATCAAAAAACATGACGATCCAACGTTACTTGGCATCGTTTGAAAATGATGGGTTAAACACCCAGAATAAACTAGAAACGCCGTGGTTTATTGCTGAAGCAATTGTCGACAGCGAGAACCCCGAGCAAATTCGGTACGAAATTCGCAAAAAGAGTGATGTTGTGGACTAG", "aa": "MVYCYEGDPVKTIKEKFLHGQFSRMPVLDQTSNEVVGFIHLKDLFSSLEKSNEPFVLQELLYPAVLVSNTTPIKQALRQMRLHRAHLAVVQDKHHHTIGIVSMEDIIEELVGEIYDEHDEVEAVQTLDNNTWLVLPNVKAAFFFNKWIKRDLVKSKNMTIQRYLASFENDGLNTQNKLETPWFIAEAIVDSENPEQIRYEIRKKSDVVD", "aa_hexdigest": "1382fc4d7ae0a4de2be93c9c47d66841", "start_type": "GTG", "rbs_motif": null, "genes": [ "mpfA" ], "truncated": "5-prime", "pseudogene": { "start": 210544, "stop": 211473, "observations": { "mutation": [ 210646, 210835 ], "stop": [ 210646, 210835 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75586", "query_cov": 1.0, "subject_cov": 0.49061032863849763, "identity": 0.995, "score": 414.0, "evalue": 6.5e-143, "gene_start": 1, "gene_end": 209, "reference_start": 218, "reference_end": 426, "reference_sequence": "MTYGKCPSGGLLALIIISIILLACISAVVSAYETAITSITSYKWSNYVKTHNKQKKLTTKIVNHFQKNYSACLITILVANNIVAILVSNILFLALDQSIKNPAISSALNLLISGVLLLMLCEITPKTLARINIIRVLVYFAVVVYFFYILFWPITKLASIIFAKYEKAPPVSRRDVYFFIDEIEQNGLFTKEDGQLIKRTLIFDQVLVDQIMIKWNRVVYCYEGDPVKTIKEKFLHGQFSRMPVLDQTSNEVVGFIHLKDLFSSLEKSNEPFVLQELLYPAVLVSNTTPIKQALRQMRLHRAHLAVVQDKHHHTIGIVSMEDIIEELVGEIYDEHDEVEAVQTLDNNTWLVLPNVKAAFFFNKWIKRDLVKSKNMTIQRYLASFENDGLNTQNKLETPWFIAEAIVDSENPEQIRYEIRKKSDVVD" }, "paralog": false, "identity": 0.9935275080906149, "score": 608.0, "evalue": 1.08e-221, "description": "Internal stop codon at 210646, 210835. Nonsense mutation around 210646, 210835.", "psc": { "uniref90_id": "UniRef90_P75586", "gene": "MpfA", "product": "UPF0053 protein MG146 homolog", "uniref50_id": "UniRef50_Q49399", "cog_id": "COG1253", "cog_category": "PT", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75586", "UniRef:UniRef50_Q49399", "COG:COG1253", "COG:PT", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q49399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49399" ], "product": "UPF0053 protein MG146" } }, "id": "GIHJHMOALN_405", "locus": "GCA009939745_00370" }, { "type": "cds", "sequence": "contig_1", "start": 211460, "stop": 212197, "strand": "+", "frame": 2, "gene": null, "product": "MPN160 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47393", "UniRef:UniRef90_P75585" ], "nt": "ATGTTGTGGACTAGAGCCTTAATCTTAGAGTTAAAAACCAATAAGCAAAGCCGCTTGTTGTGGTTACTTGCAATTCCCTTACTAATATCGTTAACCTTACTAACGTACGGTTTAGTGTTGTTTAGTTCCAGTGGTCGAATTGATCATGGTGATCACTTCCACTTACGTGAGCGCTTTGTGTTAACGACCGAAGAGTTAGTTACCTTTGTGGTAGCGAGTGTCGTGTTTGCGCTAACTGTTGCCTTGTTTGGGCTGGGTTGCTGGAAGTTGCTCCAAGGTCCAAAAGTGGATAGAACAAACATAAAGCTAGCAAACAGCAATCCTGCTCCCCAAGCCGTGGTATTGCAAGCTGATTGTGATCACTTTCAGGTAGGGGATCACTGTGTGTTTAGCGCTGAAAAGCAACACTTTAAACAACAGTTCAAACAGGACTTTTTGGGTAAAAGTAAGTTTTCCTTTCGCAATGAACTGTACCGTTTTTGTCTCATTGGTGTGTTAATTTCGCTGAATTTAGCGCTCAGTATGGTGGAAATCCCCGGCATTGTTCTACCGTGGGGCAGTTCAATCCAGTTCCGCTTTTTTAACACCGCCATCTTGTTTATTGCGATTCGCTTTGTTGGGTTACTCTCCACATCGTTAATTGCGATTATTACTCCATGGATTCACCTCCTCCTTCACCCCGTTCATACCCCGATTAGTACCGTTTTTTACATGGGCAATGACTTGGTAGTTCTGTGA", "aa": "MLWTRALILELKTNKQSRLLWLLAIPLLISLTLLTYGLVLFSSSGRIDHGDHFHLRERFVLTTEELVTFVVASVVFALTVALFGLGCWKLLQGPKVDRTNIKLANSNPAPQAVVLQADCDHFQVGDHCVFSAEKQHFKQQFKQDFLGKSKFSFRNELYRFCLIGVLISLNLALSMVEIPGIVLPWGSSIQFRFFNTAILFIAIRFVGLLSTSLIAIITPWIHLLLHPVHTPISTVFYMGNDLVVL", "aa_hexdigest": "51ce5f08758fd1099edbd881ce19b60a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 211460, "stop": 212500, "observations": { "mutation": [ 212195 ], "stop": [ 212195 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75585", "query_cov": 1.0, "subject_cov": 0.649867374005305, "identity": 1.0, "score": 471.0, "evalue": 1.61e-165, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 245, "reference_sequence": "MLWTRALILELKTNKQSRLLWLLAIPLLISLTLLTYGLVLFSSSGRIDHGDHFHLRERFVLTTEELVTFVVASVVFALTVALFGLGCWKLLQGPKVDRTNIKLANSNPAPQAVVLQADCDHFQVGDHCVFSAEKQHFKQQFKQDFLGKSKFSFRNELYRFCLIGVLISLNLALSMVEIPGIVLPWGSSIQFRFFNTAILFIAIRFVGLLSTSLIAIITPWIHLLLHPVHTPISTVFYMGNDLVVLWIFYFFYYHIFKAEVKQTTTVVNNKEFSQLVNTHKTKVAKALALIPVNLICGFIEGLGFYVGYFLILGKFGSVGHKIFYDSQANRDLINSANVIYFLLTTTTIFSLKYLFELLFFYSVEKGILNISRHFGLY" }, "paralog": false, "identity": 0.9971098265895953, "score": 687.0, "evalue": 3.18e-253, "description": "Internal stop codon at 212195. Nonsense mutation around 212195.", "psc": { "uniref90_id": "UniRef90_P75585", "product": "MPN160 family protein", "uniref50_id": "UniRef50_P47393", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75585", "UniRef:UniRef50_P47393", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47393" ], "product": "Uncharacterized protein MG147" } }, "id": "GIHJHMOALN_406", "locus": "GCA009939745_00371" }, { "type": "cds", "sequence": "contig_1", "start": 212318, "stop": 212593, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCGTTAATTCCAGTTAATTTAATCTGTGGTTTTATTGAGGGTTTGGGTTTCTATGTCGGTTACTTCTTGATTTTGGGTAAATTTGGTAGTGTAGGGCATAAGATTTTCTATGACAGTCAAGCTAACCGTGACCTAATTAACAGTGCTAACGTGATCTATTTCTTACTCACTACTACCACCATTTTTAGCTTAAAGTACCTGTTTGAACTACTGTTCTTTTACAGTGTCGAGAAGGGTATACTTAACATTTCGCGCCACTTTGGTTTGTATTAA", "aa": "MALIPVNLICGFIEGLGFYVGYFLILGKFGSVGHKIFYDSQANRDLINSANVIYFLLTTTTIFSLKYLFELLFFYSVEKGILNISRHFGLY", "aa_hexdigest": "11c90ec030d761a2f8b55e1f1a9cbb06", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10391.103999999994, "isoelectric_point": 7.863224983215332 }, "id": "GIHJHMOALN_407", "locus": "GCA009939745_00372" }, { "type": "cds", "sequence": "contig_1", "start": 212747, "stop": 212998, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGACGTCAAATACTTACAGGTCGAAATTGTCGATAAAAACACCATTCGCCTCCAGGAAGATGGCAAAAAGGGTCAACAAATTAGGTTAGATCAAATTATTAAGGTTGATCAAAGCAACATCTTAAACACTTTGGAGCAGGCACAACGCGAGGCATACGAAAGGGAGGCGCAAAGCCGTTACCAAACTAAGTTGGCCAAAGAACTGAGTGAAAAAGACAACACTTTTTTAAAGCAAGAAGCAGCTTGA", "aa": "MSDVKYLQVEIVDKNTIRLQEDGKKGQQIRLDQIIKVDQSNILNTLEQAQREAYEREAQSRYQTKLAKELSEKDNTFLKQEAA", "aa_hexdigest": "6b6a89f0b9f9f73823c7475d6f530285", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9712.791099999993, "isoelectric_point": 5.46429615020752 }, "id": "GIHJHMOALN_408", "locus": "GCA009939745_00373" }, { "type": "cds", "sequence": "contig_1", "start": 213179, "stop": 213325, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATACGAACGTAAAGCAAATAAAACTAAGGAAGAAAATCAACAAGAATTAGAAAGACAACGCCAGTACTTTTTAGAACAGTTAGAACAAGCCCAAAAGGACATTGAAGAGCTCAAAACGCGTGAAGAGCGTTTTAGTAAGTGA", "aa": "MEYERKANKTKEENQQELERQRQYFLEQLEQAQKDIEELKTREERFSK", "aa_hexdigest": "d147e97b9e9c59a795c2412b96d1a0c6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6106.661499999998, "isoelectric_point": 5.169983863830565 }, "id": "GIHJHMOALN_409", "locus": "GCA009939745_00374" }, { "type": "cds", "sequence": "contig_1", "start": 213350, "stop": 214084, "strand": "+", "frame": 2, "gene": null, "product": "putative MG148-like protein", "db_xrefs": [ "COG:COG4487", "COG:S", "SO:0001217", "UniRef:UniRef50_P75584", "UniRef:UniRef90_P75584" ], "nt": "TTGGAAAAGTGGTGTTGGGAAGCTTACAAAAATTACGAAGAGTTGTTCCAAAACTGTGTGTTTGCCCCTTACAGTGAGCTAGTTAAGGGTGCCAAAAAGAGCATAGGTGTAGAAGACGATGAGAGCAATATTAACGAAAAGGCTGACTTTATCTTCCAGGTATTTAATCCCAATAATGACAAAGAACCCTTCTTTTCAATTTGCTGTGAAATGAAAACAGAGTTTACTGAAAGCAAGAGTCGCACTAAAAACGAAGATCATGTCAAAAAACTGATTGCCGACGCTAAGCGCGCTAAGTGCCAATACGGTTTTTTAGTAAGTGAATTGGAACTCAACACTGAAAACGATGTCCAAGTGCAACGGATGCATACCTCGAACAGTGAAGTGGAGGTTTACCTAGTGCGCCCAATGTTCTTCATTGTGATGTTGCGGTTGTTTTACTTTTTGGCCAAAAAGATGTTTGCCCAAGTTGATGTAAACAGCGAGTACTTAGATAAAGAAGCTTTAAATGCTAGCTTTAGCGATTTAAAGAAAAGCTTACTTGAAAAAACCTTTACTGATCTCAACAAGGTCTTTCAAAACAACATTGATGAACTAGAAAAGATTGAAGGATTAGTAACAAAGTTGAAAGCCGCTAATGAAAAGGCCTTAAATAGTCGTTTAAATAACTGGGAAGAAAAGATCCGTAAGTTTGAATTTAAGCTCAATAAAGACATTGTTAAAAAGCTTGAATAA", "aa": "MEKWCWEAYKNYEELFQNCVFAPYSELVKGAKKSIGVEDDESNINEKADFIFQVFNPNNDKEPFFSICCEMKTEFTESKSRTKNEDHVKKLIADAKRAKCQYGFLVSELELNTENDVQVQRMHTSNSEVEVYLVRPMFFIVMLRLFYFLAKKMFAQVDVNSEYLDKEALNASFSDLKKSLLEKTFTDLNKVFQNNIDELEKIEGLVTKLKAANEKALNSRLNNWEEKIRKFEFKLNKDIVKKLE", "aa_hexdigest": "0d53eec1d7093812876b189bce4ba508", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 213050, "stop": 214084, "observations": { "mutation": [ 213323 ], "stop": [ 213323 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75584", "query_cov": 1.0, "subject_cov": 0.5483146067415731, "identity": 0.996, "score": 470.0, "evalue": 3.33e-164, "gene_start": 1, "gene_end": 244, "reference_start": 202, "reference_end": 445, "reference_sequence": "MSDVKYLQVEIVDKNTIRLQEDGKKGQQIRLDQIIKVDQSNILNTLEQAQREAYEREAQSRYQTKLAKELSEKDNTFLKQKAAWNQAHNTQIQQLYQQITNLENQVNNIKRETESKKDNEYQQQIVKLETQLQSIKKETESQKDLEYERKANKTKEENQQELERQRQYFLEQLEQAQKDIEELKTREERFSKWAIAKKGKELEKWCWEAYKNYEELFQNCVFAPYSELVKGAKKSIGVEDDESNINEKADFIFQVFNPNNDKEPFFSICCEMKTEFTESKSRTKNEDHVKKLIADAKRAKCQYGFLVSELELNTENDVQVQRMHTSNSEVEVYLVRPMFFIVMLRLFYFLAKKMFAQVDVNSEYLDKEALNASFSDLKKSLLEKTFTDLNKVFQNNIDELEKIEGLVTKLKAANEKALNSRLNNWEEKIRKFEFKLNKDIVKKLE" }, "paralog": false, "identity": 0.9767441860465116, "score": 620.0, "evalue": 1.14e-225, "description": "Internal stop codon at 213323. Nonsense mutation around 213323.", "psc": { "uniref90_id": "UniRef90_P75584", "product": "Uncharacterized protein MG148 homolog", "uniref50_id": "UniRef50_P75584", "cog_id": "COG4487", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75584", "UniRef:UniRef50_P75584", "COG:COG4487", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_P75584", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75584" ], "product": "Uncharacterized protein MG148 homolog" } }, "id": "GIHJHMOALN_410", "locus": "GCA009939745_00375" }, { "type": "cds", "sequence": "contig_1", "start": 214206, "stop": 214559, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTAAATCTGCGATTTCCTAGCTATTTTTTACCCGTCGTTGCCGCTAGTGCTTTCCTTGTTAGTTGCGCCACACCTAACACTTACGAAGTACAACGCGCTGCCTTGATTCAACTAGTTGAAGAAGACGAACGACAAAACTACATTCAAAAGGGTAGTATGGGTGCAAATGCTGTTATGACTGCTGCAAAAGCAGAAACCAAAACAGCAGAAAAAACAGCAACTTCTACCAAAGCTGCTTCAATAGAACTTAAAAAAACTGATACTGATATTAAGACTACAACTACTACCGAAAACAAATCAGCATCCGGATACAAACTAGATACCCTCTTTGGTGACTACATCTTGTGA", "aa": "MKLNLRFPSYFLPVVAASAFLVSCATPNTYEVQRAALIQLVEEDERQNYIQKGSMGANAVMTAAKAETKTAEKTATSTKAASIELKKTDTDIKTTTTTENKSASGYKLDTLFGDYIL", "aa_hexdigest": "a457cf17742fb2ebbb26bf4b0fb3dd76", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12752.366599999976, "isoelectric_point": 7.770879173278809 }, "id": "GIHJHMOALN_411", "locus": "GCA009939745_00376" }, { "type": "cds", "sequence": "contig_1", "start": 214650, "stop": 215168, "strand": "+", "frame": 3, "gene": null, "product": "MPN162 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75583", "UniRef:UniRef90_P75583" ], "nt": "GTGATTTTAGACGGTGGTAACTTGACCGTGGAAAAAAATCATGAACATGGCCATACTCATAAAAATGGTGAAACTCACGAACATGATCATGATCACCACGAAGGTGAAGAGGAAGTAATCGTTGGTCGTGCCCTTTCTTTTGCTAATGGTCTCTTCTTAGTTGTGGATCTTAAGGAAGAAAAACACGAAGAGAAGAAGGAAGCAAAATCTGAAATGTCAATGAACAGTAAAGATATGGTGATGATGACCAAAACTGAAATGATGTCTAAGGAAATGAAATCCGAACAAAAAATGGAGAAGAAGGAGGAACACGAACATCATCACAAAAAGCTGAGCCTCAGTACCACGGCTTACAAGTTTGGTCAAAGCTTTAACATCCTGGAATTTACTGGTGCTATGCACCACAAAACGGCACACAATAACGAAACCGAATTTAAAAATTTAGGCAAAAAATACGGTGGTATGACCGAGTACACGATTGTCGACTTTGACTTTAATCCACCAAAACCAACTAAGTAA", "aa": "MILDGGNLTVEKNHEHGHTHKNGETHEHDHDHHEGEEEVIVGRALSFANGLFLVVDLKEEKHEEKKEAKSEMSMNSKDMVMMTKTEMMSKEMKSEQKMEKKEEHEHHHKKLSLSTTAYKFGQSFNILEFTGAMHHKTAHNNETEFKNLGKKYGGMTEYTIVDFDFNPPKPTK", "aa_hexdigest": "431194463bd7e667d33c3c1996538570", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 214350, "stop": 215168, "observations": { "mutation": [ 214557 ], "stop": [ 214557 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75583", "query_cov": 1.0, "subject_cov": 0.5375, "identity": 0.9590000000000001, "score": 260.0, "evalue": 1.19e-84, "gene_start": 1, "gene_end": 172, "reference_start": 149, "reference_end": 320, "reference_sequence": "MKLNLRFPSYFLPVVAASAFLVSCATPNTYEVQRAALIQLVEEDERQNYIQKGSMGANAVMTAAKAETKTAEKTATSTKAASIELKKTDTDIKTTTTTENKSASGYKLDTLFGDYILWVVDHLSGLLFSPKTNNSTTTQKIQLITEDKMILDGGNLTVEKNHEHGHTHKNGETHEHDHDHHEGEEEVIVGRALSFANGLFLVVDLKEEKHEEKKEAKSEMSMNSKDMVMMTKTEMMSKEMKSEQKMEKKEEHEHPHKKLSLSTTAYKFGQSFNILEFTGAMHHKTAHNNETEFKNLGKKYGGMTEYTIVDFDFNPPKPTK" }, "paralog": false, "identity": 0.9669117647058824, "score": 412.0, "evalue": 1.43e-146, "description": "Internal stop codon at 214557. Nonsense mutation around 214557.", "psc": { "uniref90_id": "UniRef90_P75583", "product": "MPN162 family protein", "uniref50_id": "UniRef50_P75583", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75583", "UniRef:UniRef50_P75583", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75583", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75583" ], "product": "Uncharacterized lipoprotein MG149 homolog" } }, "id": "GIHJHMOALN_412", "locus": "GCA009939745_00377" }, { "type": "cds", "sequence": "contig_1", "start": 215367, "stop": 215753, "strand": "+", "frame": 3, "gene": null, "product": "MPN163 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014574900.1", "SO:0001217", "UniParc:UPI0001DD64DA", "UniRef:UniRef100_A0A0H3DMB4", "UniRef:UniRef50_Q9ZB80", "UniRef:UniRef90_P75582" ], "nt": "TTGGAAAAAGATAAGGAATTAGTCAGTTCAATTGTGACTGCTAAAAGCATGATTGACCGGTTTTTCTGGTCTATTTTGAGCTTTCTGCTCACTAACTTAGTCTTTTTATTTGTGGCTTTTTTAATTCTGATTATTTACTTAATTTCCGAAATCACCCAACAGTTTGCCTTTGCTTTTATCGCAACGATTGTCTTCATCATTTTTTACAACATCCTCTTTTTGAGCTACCTGTTGACGATGTACATTAAAGGCTTAAAACAGATTGAGCAAAAGTCACGTTATTTACTCTTAGTGCTTGATGTTAAGGCTGATGAACTGCTTCCTTTCTCCTTTTTAGGTAGTTTAAGAAAGAGTCACATGCTTGAAGAAATGTTGTTAGAGCAGTAA", "aa": "MEKDKELVSSIVTAKSMIDRFFWSILSFLLTNLVFLFVAFLILIIYLISEITQQFAFAFIATIVFIIFYNILFLSYLLTMYIKGLKQIEQKSRYLLLVLDVKADELLPFSFLGSLRKSHMLEEMLLEQ", "aa_hexdigest": "a53a8e4bcd321239e32ee769b1a386f7", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64DA", "ncbi_nrp_id": "WP_014574900.1", "uniref100_id": "UniRef100_A0A0H3DMB4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64DA", "RefSeq:WP_014574900.1", "UniRef:UniRef100_A0A0H3DMB4" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DMB4", "uniref90_id": "UniRef90_P75582" }, "psc": { "uniref90_id": "UniRef90_P75582", "product": "MPN163 family protein", "uniref50_id": "UniRef50_Q9ZB80", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB80" ], "product": "Uncharacterized protein MG149.1" }, "genes": [], "id": "GIHJHMOALN_413", "locus": "GCA009939745_00378" }, { "type": "cds", "sequence": "contig_1", "start": 215847, "stop": 216173, "strand": "+", "frame": 3, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627", "RefSeq:WP_010874521.1", "SO:0001217", "UniParc:UPI0000134BB6", "UniRef:UniRef100_P75581", "UniRef:UniRef50_O52331", "UniRef:UniRef90_P75581" ], "nt": "ATGAACGCCGCTAACGCAGTTAAATACCCTGAGCTCAAGATTAAGCTCGAGTCCTATGACAGCACCCTTTTAGACCTCACTACGAAAAAGATAGTGGAGGTAGTAAAGGGTGTCGATGTCAAAATTAAAGGTCCATTGCCTCTACCTACCAAAAAAGAGGTAATTACGATCATCCGTTCACCCCATGTGGATAAAGCATCACGCGAACAGTTTGAAAAAAACCGCCACAAACGTTTAATGATCTTGGTGGATGTTAACCAAGGTGCGATTGACAGCTTGAAGCGGATCAAGATCCCCGTTGGAGTAACTTTAAGATTTTCAAAGTAG", "aa": "MNAANAVKYPELKIKLESYDSTLLDLTTKKIVEVVKGVDVKIKGPLPLPTKKEVITIIRSPHVDKASREQFEKNRHKRLMILVDVNQGAIDSLKRIKIPVGVTLRFSK", "aa_hexdigest": "24ae859f4f5db52be5dbf177e84f99ca", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134BB6", "ncbi_nrp_id": "WP_010874521.1", "uniref100_id": "UniRef100_P75581", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134BB6", "RefSeq:WP_010874521.1", "UniRef:UniRef100_P75581" ] }, "ips": { "uniref100_id": "UniRef100_P75581", "uniref90_id": "UniRef90_P75581" }, "psc": { "uniref90_id": "UniRef90_P75581", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_O52331", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52331", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52331" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "GIHJHMOALN_414", "locus": "GCA009939745_00379" }, { "type": "cds", "sequence": "contig_1", "start": 216187, "stop": 217050, "strand": "+", "frame": 1, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "RefSeq:WP_014325386.1", "SO:0001217", "UniParc:UPI0002385E50", "UniRef:UniRef100_A0AB33HMP0", "UniRef:UniRef50_P75580", "UniRef:UniRef90_P75580" ], "nt": "ATGGAAATTCGTGGTATTTTTGGGGTTAAAGTAGGCATGAGCCAGGTGTTTACAACCAATAATGAGCGCCTACCTATTACCGTTATTTATTGTGAACCTAACCAGGTAGCAGGTGTTAAGAATGAGGCTAAGGACAAGTACAGTGCTACCTTGTTGAGTTTTGACACCGTTGAAAACAAAAAGCTCAACAAGCCTCAACAAGGCTTCTTTGAAAAAAACAACTTAAAGCCAACCAAGCACTTACAGGAAATCCGCAATATGACCGGGTTTGAAATGGGGCAACAAATCACACCGCAAAATCTCTTCCAAGTGGGTGAGTATGTTGATGTAAGTGCCATTAGTAAAGGTCGTGGGTTTACTGGTGCCATTAAGCGCTGGAACTTTAAGATCGGTCCGTTAGGTCATGGGGCTGGTTATCCCCACCGCTTCCAAGGTTCAGTCCAAGCTGGTCGTGGGGGTGCGAGTGCACAAAGAGTGTTTAAGGGCAAAAAGATGTCCGGACACTATGGTCATGAAAAAGTAACGGTACAAAACTTACGCATTGTTGGTTTTGATGAAGCTAACATGTTGGTATTAGTATCAGGGGCGATCGCAGGTCCTGAAGGTGGTGTGGTCTTCATCCGTACCGCTAAGAAAAAACCGGGGGTAGTGAAACCAATTGAGTTAGCTGTTCAAACCGAAAAGGCACCGGAAGCAAAACCGGCCAAGCTTTCCAAAAAGAAACAAGCCAAGGAGTTAGCGAAAGCACAAGCAGCTAACCAGCAAACTGTTGAAGCTAAGGTAGACACTCCAGTAGTAGAACCAAAACCAACTGAAGTTAAAAAAGCAGCACCAGTTGTAGAAAAGAAAGGGAAAGATAAATAA", "aa": "MEIRGIFGVKVGMSQVFTTNNERLPITVIYCEPNQVAGVKNEAKDKYSATLLSFDTVENKKLNKPQQGFFEKNNLKPTKHLQEIRNMTGFEMGQQITPQNLFQVGEYVDVSAISKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGASAQRVFKGKKMSGHYGHEKVTVQNLRIVGFDEANMLVLVSGAIAGPEGGVVFIRTAKKKPGVVKPIELAVQTEKAPEAKPAKLSKKKQAKELAKAQAANQQTVEAKVDTPVVEPKPTEVKKAAPVVEKKGKDK", "aa_hexdigest": "7a836fb8343b949ba8c8684089464daa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002385E50", "ncbi_nrp_id": "WP_014325386.1", "uniref100_id": "UniRef100_A0AB33HMP0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002385E50", "RefSeq:WP_014325386.1", "UniRef:UniRef100_A0AB33HMP0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HMP0", "uniref90_id": "UniRef90_P75580" }, "psc": { "uniref90_id": "UniRef90_P75580", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_P75580", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75580", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75580" ], "product": "Large ribosomal subunit protein uL3" }, "genes": [ "rplC" ], "id": "GIHJHMOALN_415", "locus": "GCA009939745_00380" }, { "type": "cds", "sequence": "contig_1", "start": 217050, "stop": 217184, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAGCTTAAACTAATTAAAATCGACGGTTCCTTTGAAACTGAACCAGTGAAACTAAGCCCTGGTTTAATTGCTAAGGAACTCAAACAACAACCGGTGTTTGATGCCGTGTTAGTAGAACAAGCTTCCTGA", "aa": "MAKLKLIKIDGSFETEPVKLSPGLIAKELKQQPVFDAVLVEQAS", "aa_hexdigest": "606d57c287ba36a74d465242d00bebc1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4810.649100000002, "isoelectric_point": 6.090491294860839 }, "id": "GIHJHMOALN_416", "locus": "GCA009939745_00381" }, { "type": "cds", "sequence": "contig_1", "start": 217317, "stop": 217688, "strand": "+", "frame": 3, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:0046677", "GO:1990904", "SO:0001217", "UniRef:UniRef50_P75579", "UniRef:UniRef90_P75579" ], "nt": "GTGTTTGGTCCCAAGCCCAATCGTAACTACAGCTTAAAACTCAACAAGAAAGCGCACACGGCAGCCTTGCACACTGTTTGGAGTGAAAAGTTAGCTTCAGACAACACCCACCTAGTGGACCAAAACCTCTTTAACAAAACTGAGGGTAAAACCAAGGTGATGGTGCAATTTTTAAAGAGTGCCAAGCTCTTGGACAAAAACGTGTTGTTTGTAGTTAATACCCTTAACACCAACTTAGAACAAAGCACCAGCAACATTAAAAACGTGCAGGTCAAACACCTAGACAAGGTTTCTGTAAGGGATCTGATGTTAGCTAACGCTCTTTTAGTTGAAAAAGAAGTTTTAAAAGCTTTGGAGGGCAAATTTAAATAA", "aa": "MFGPKPNRNYSLKLNKKAHTAALHTVWSEKLASDNTHLVDQNLFNKTEGKTKVMVQFLKSAKLLDKNVLFVVNTLNTNLEQSTSNIKNVQVKHLDKVSVRDLMLANALLVEKEVLKALEGKFK", "aa_hexdigest": "d8daaef8ac53e53dfb3e1f423f3bea66", "start_type": "GTG", "rbs_motif": null, "genes": [ "rplD" ], "truncated": "5-prime", "pseudogene": { "start": 217050, "stop": 217688, "observations": { "mutation": [ 217182 ], "stop": [ 217182 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75579", "query_cov": 1.0, "subject_cov": 0.5801886792452831, "identity": 0.9840000000000001, "score": 234.0, "evalue": 1.08e-76, "gene_start": 1, "gene_end": 123, "reference_start": 90, "reference_end": 212, "reference_sequence": "MAKLKLIKIDGSFETEPVKLSPGLIAKELKQQPVFDAVLVEQASWRQGTHSILTKGEVRGGGKKPYKQKHTGKARQGSTRNPHFVGGGIVFGPKPNRNYSLKLNKKAHTAALHTVWSEKLASDNTHLVDQNLFNKTEGKTKVMMQFLKSAKLLDKNVLFVVNTLNTNLEQSTSNIKNVQVKHLDKVSVRDLMLANALLVEKEVLKALEGKFK" }, "paralog": false, "identity": 0.9905660377358491, "score": 412.0, "evalue": 2.39e-149, "description": "Internal stop codon at 217182. Nonsense mutation around 217182.", "psc": { "uniref90_id": "UniRef90_P75579", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_P75579", "cog_id": "COG0088", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:0046677", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75579", "UniRef:UniRef50_P75579", "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:0046677", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P75579", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75579" ], "product": "Large ribosomal subunit protein uL4" } }, "id": "GIHJHMOALN_417", "locus": "GCA009939745_00382" }, { "type": "cds", "sequence": "contig_1", "start": 217691, "stop": 218404, "strand": "+", "frame": 2, "gene": "rplW", "product": "Large ribosomal subunit protein uL23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874524.1", "SO:0001217", "UniParc:UPI0000133DA4", "UniRef:UniRef100_P75578", "UniRef:UniRef50_P75578", "UniRef:UniRef90_P75578" ], "nt": "ATGGATGTAACGAACGTACTGCTAAAACCAGTTCTAACCGAAAAGGTGTACTTCAACCAAATGGGTGAAACCAAGAAGTACGTCTTTGTGGTGAACCCTAAAGCAAGTAAAACACGCGTTAAACTAGCTTTTGAATTAGTGTATGGCATTAAGCCACTGAAGGTTAACACCTTAATTAGAAAACCAACCACCATTCGCGGTGGGAGCCGTTTTCCTGGTTTGAGCAAACTAGAAAAGTTGGCTGTGATTACCTTACCAAAGGGTATTGCCATTTCAGTTACTGGTGAAGCGCCAGAAAAAACCGATAAGCCAGCGGACAAAACCACCCTTAAGGAAAGTACTGTTAAGGAGATTAAGGACACCAAAAACAGTCCAGAAGCTGTTGTGAAAACTGCTGTGGAAGCGTTGCAAATTAAACCAACTGCCGCTCCTGTAACCACCGCCCCTCTACAAACCGTGGCGGTGAAGGTAGCTAAAGAAGTTAAAGAAGTTAAGGTAGAAAAGCCGGTTAAAGTGGAAAAACCAACCAAACCGGCAAAGGTAGCAAAAGAAGCTAAAACGACCAAAGTAGCTAAGGAAACTAAAGCTGAAAAGTCAGTTCAAACAACCAAAGTAGCTAAGGAAACTAAAACTGAAAAGTCAGCTAAAACTACCAAAACTACAGCTACAAAAACTACTAAAACTAAAACCACCAAAAAGGAGGTTAAAAAGTAA", "aa": "MDVTNVLLKPVLTEKVYFNQMGETKKYVFVVNPKASKTRVKLAFELVYGIKPLKVNTLIRKPTTIRGGSRFPGLSKLEKLAVITLPKGIAISVTGEAPEKTDKPADKTTLKESTVKEIKDTKNSPEAVVKTAVEALQIKPTAAPVTTAPLQTVAVKVAKEVKEVKVEKPVKVEKPTKPAKVAKEAKTTKVAKETKAEKSVQTTKVAKETKTEKSAKTTKTTATKTTKTKTTKKEVKK", "aa_hexdigest": "d08555cd895ec3b00883a94da7e8e52e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DA4", "ncbi_nrp_id": "WP_010874524.1", "uniref100_id": "UniRef100_P75578", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DA4", "RefSeq:WP_010874524.1", "UniRef:UniRef100_P75578" ] }, "ips": { "uniref100_id": "UniRef100_P75578", "uniref90_id": "UniRef90_P75578" }, "psc": { "uniref90_id": "UniRef90_P75578", "gene": "rplW", "product": "Large ribosomal subunit protein uL23", "uniref50_id": "UniRef50_P75578", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:1990904", "GO:0005840", "GO:0019843", "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P75578", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75578" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "GIHJHMOALN_418", "locus": "GCA009939745_00383" }, { "type": "cds", "sequence": "contig_1", "start": 218404, "stop": 219267, "strand": "+", "frame": 1, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "COG:COG0090", "COG:J", "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843", "KEGG:K02886", "RefSeq:WP_010874525.1", "SO:0001217", "UniParc:UPI0000133ECF", "UniRef:UniRef100_P75577", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_P75577" ], "nt": "ATGCCTATTAAGAAAATTATTAGTCGTTCTAACAGTGGGATCCACCACTCCACGGTAATTGACTATAAGAAGTTATTAACCACCAATAAGAACAAACCAGAAAAGTCGTTGTTAGTTACCCTTAAAAAACACGGGGGTCGCAACAACCAAGGGAAAATTACGGTGCGCCACCAGGGTGGACGCAACAAGCGCAAGTACCGCATTATTGACTTTAAACGAACGCACTATGACAACATCGAAGCGACCGTTAAGTCGATCGAGTACGATCCGAACCGTTCTTGCTTTGTGTCGTTAATTACTTACGCTAACGGTGCCAAGAGTTACATCATTAGCCCTGATGGCATTAAGGTAGGTGATAAGATCTTGGCTTCCGAACACCCGATTGACATTAAACCGGGCTTTAGTATGCCCTTAGCCTTTATCCCTGAAGGTACCCAAGTGCATAACATTGAACTCCACCCTAAGGGAGGTGGTCAAATTGCCAGGAGTGCGGGGAGTTATGCCCGCATTTTGGGTCAAGACGAAACTGGTAAGTATGTCATTTTGCAACTTTTAAGTGGTGAAACACGCAAGTTCTTAAAGGAGTGCCGTGCTACTGTGGGAGTAGTTTCTAACTTAGACCACAACCTCGTAGTCATTGGTAAGGCTGGTCGCAACCGTCACCGCGGCATCCGTCCAACCGTTCGTGGTTCGGCCATGAACCCCAATGACCACCCCCACGGTGGTGGTGAAGGTCGTAGTCCCGTGGGTCGGGATGCACCCCGGACTCCATGGGGTAAGCGCCACATGGGTGTGAAGACCCGCAACATGAAGAAAGCATCCACTAACTTAATTATTCGTAACCGGAAAGGAGAACAATACTAA", "aa": "MPIKKIISRSNSGIHHSTVIDYKKLLTTNKNKPEKSLLVTLKKHGGRNNQGKITVRHQGGRNKRKYRIIDFKRTHYDNIEATVKSIEYDPNRSCFVSLITYANGAKSYIISPDGIKVGDKILASEHPIDIKPGFSMPLAFIPEGTQVHNIELHPKGGGQIARSAGSYARILGQDETGKYVILQLLSGETRKFLKECRATVGVVSNLDHNLVVIGKAGRNRHRGIRPTVRGSAMNPNDHPHGGGEGRSPVGRDAPRTPWGKRHMGVKTRNMKKASTNLIIRNRKGEQY", "aa_hexdigest": "84ec1e77c99929a1da5f4d6fe0e5aad7", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI0000133ECF", "ncbi_nrp_id": "WP_010874525.1", "uniref100_id": "UniRef100_P75577", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133ECF", "RefSeq:WP_010874525.1", "UniRef:UniRef100_P75577" ] }, "ips": { "uniref100_id": "UniRef100_P75577", "uniref90_id": "UniRef90_P75577" }, "psc": { "uniref90_id": "UniRef90_P75577", "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "cog_id": "COG0090", "cog_category": "J", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "GIHJHMOALN_419", "locus": "GCA009939745_00384" }, { "type": "cds", "sequence": "contig_1", "start": 219267, "stop": 219530, "strand": "+", "frame": 3, "gene": "rpsS", "product": "30S ribosomal protein S19", "db_xrefs": [ "COG:COG0185", "COG:J", "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "RefSeq:WP_010874526.1", "SO:0001217", "UniParc:UPI0000134DEC", "UniRef:UniRef100_P75576", "UniRef:UniRef50_Q6ME59", "UniRef:UniRef90_P75576" ], "nt": "ATGTCACGCAGTGCTAAAAAAGGAGCCTTTGTTGATGCTCACCTCTTAAAGAAAGTGATCGATATGAACAAACAAGAGAAAAAACGTCCGATCAAAACCTGGTCCCGTCGTAGTACCATCTTCCCCGAATTTGTCGGTAATACCTTTGCTGTACATAACGGCAAGACCTTCATTAATGTTTATGTAACTGATGACATGGTGGGGCACAAACTCGGTGAGTTCTCCCCGACCCGTAACTTCAAACAACACACCGCTAACCGTTAG", "aa": "MSRSAKKGAFVDAHLLKKVIDMNKQEKKRPIKTWSRRSTIFPEFVGNTFAVHNGKTFINVYVTDDMVGHKLGEFSPTRNFKQHTANR", "aa_hexdigest": "9fa790bb912197d37c1ffb6630f98a3c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134DEC", "ncbi_nrp_id": "WP_010874526.1", "uniref100_id": "UniRef100_P75576", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DEC", "RefSeq:WP_010874526.1", "UniRef:UniRef100_P75576" ] }, "ips": { "uniref100_id": "UniRef100_P75576", "uniref90_id": "UniRef90_P75576" }, "psc": { "uniref90_id": "UniRef90_P75576", "gene": "rpsS", "product": "30S ribosomal protein S19", "uniref50_id": "UniRef50_Q6ME59", "cog_id": "COG0185", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q6ME59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6ME59" ], "product": "Small ribosomal subunit protein uS19" }, "genes": [ "rpsS" ], "id": "GIHJHMOALN_420", "locus": "GCA009939745_00385" }, { "type": "cds", "sequence": "contig_1", "start": 219532, "stop": 220011, "strand": "+", "frame": 1, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "GO:0046677", "RefSeq:WP_014574902.1", "SO:0001217", "UniParc:UPI0001DD64DD", "UniRef:UniRef100_A0AAP8JFH9", "UniRef:UniRef50_P75575", "UniRef:UniRef90_P75575" ], "nt": "ATGATCGCCTTTGCAAAACAATTCCGTGTGAGAATCTCACCCCAAAAAGCCCGCTTAGTGTGTCAACTCATTGTGGGTAAGAAAACCGCTGATGCCCAAAACATCCTTTCCAATACACCCAAAAAAGCGGCTACCTTAATCGCTAAACTACTCAACAGTGCGATTGCCAACGCTACCAATAACCACGGCATGAATGGGGATGCCCTTTATGTCTTTGAGTGTGTGGCTAACCAAGGTCCGAGTATGAAGCGCACTATTCCCCGCGCTAAGGGCTCGAGTAACATGATTACCAAGCGCTCTTCTAATTTAGTGGTGAAACTATCGGATAACCCGAATGAACGTCAAGAATTAATTAAACAACAAAAGGCCCTAGTTAAAAAACGGGTCGAAGGTCAACAAAAAGCAAAGATGGCACGTCAAAAAGCAGTAACACCAGTGGTAAAAGCGCCAAGTAAAACTCAAGGAGGTGTACAAAAGTAA", "aa": "MIAFAKQFRVRISPQKARLVCQLIVGKKTADAQNILSNTPKKAATLIAKLLNSAIANATNNHGMNGDALYVFECVANQGPSMKRTIPRAKGSSNMITKRSSNLVVKLSDNPNERQELIKQQKALVKKRVEGQQKAKMARQKAVTPVVKAPSKTQGGVQK", "aa_hexdigest": "d2a87f364f2e381fc97661d6c0982c33", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64DD", "ncbi_nrp_id": "WP_014574902.1", "uniref100_id": "UniRef100_A0AAP8JFH9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64DD", "RefSeq:WP_014574902.1", "UniRef:UniRef100_A0AAP8JFH9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8JFH9", "uniref90_id": "UniRef90_P75575" }, "psc": { "uniref90_id": "UniRef90_P75575", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_P75575", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "GO:0046677" ] }, "pscc": { "uniref50_id": "UniRef50_P75575", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75575" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "GIHJHMOALN_421", "locus": "GCA009939745_00386" }, { "type": "cds", "sequence": "contig_1", "start": 220178, "stop": 220834, "strand": "+", "frame": 2, "gene": "rpsC", "product": "30S ribosomal protein S3", "db_xrefs": [ "COG:COG0092", "COG:J", "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627", "KEGG:K02982", "SO:0001217", "UniRef:UniRef50_O52338", "UniRef:UniRef90_P41205" ], "nt": "GTGGAGATTGAACGTACTCAAGCAACCGTGGACGTATTTGTGTACGCCGCCCAACCCGCTTTTCTCATTGGTAGTGAAAACAAGAACATCCAAAAGATCACCAAGCAAATTAAGCAAATTATTGGTCGTACCACCAATTTAGACCTCACCATTAACGAAATTGGCTCCCCTATGTTATCAGCGCGCATTATTGCGCGGGATCTAGCCAACGCGATTGAAGCACGGGTACCGTTACGAACGGCAATGCGCCAATCCTTAATTAAGGTGTTGAAGGCTGGCGCTAACGGGATTAAGGTGTTGGTATCGGGTCGGTTAAACGGTGCCGAAATTGCGCGCGACAAGATGTACATCGAGGGCAATATGCCACTGTCAACCCTGCGTGCTGACATTGACTATGCCTTGGAAAAAGCGCAAACTACGTACGGTGTCATTGGTGTTAAGGTATGGATTAATCGCGGCATGATTTACACCAAGGGCTTAAACCGCACCCCGGCCCACATTCTCCACCCACAAAAGAAACAACCAAACCGCCAAAACCAACAACCACGCCACTTTAACCAGGGCCAGGTGTTGAGTGCTAATAAGTTAACTGGTAGTGATGTGGAGACCAGCAGTATCCAAGCACTTACTAAGCCTAACAAGGAGGACAAACAATAA", "aa": "MEIERTQATVDVFVYAAQPAFLIGSENKNIQKITKQIKQIIGRTTNLDLTINEIGSPMLSARIIARDLANAIEARVPLRTAMRQSLIKVLKAGANGIKVLVSGRLNGAEIARDKMYIEGNMPLSTLRADIDYALEKAQTTYGVIGVKVWINRGMIYTKGLNRTPAHILHPQKKQPNRQNQQPRHFNQGQVLSANKLTGSDVETSSIQALTKPNKEDKQ", "aa_hexdigest": "93133787fde845f73905639b469b8ff7", "start_type": "GTG", "rbs_motif": null, "genes": [ "rpsC" ], "truncated": "5-prime", "pseudogene": { "start": 220013, "stop": 220834, "observations": { "mutation": [ 220076 ], "stop": [ 220076 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P41205", "query_cov": 1.0, "subject_cov": 0.7985347985347986, "identity": 0.995, "score": 413.0, "evalue": 6.56e-145, "gene_start": 1, "gene_end": 218, "reference_start": 56, "reference_end": 273, "reference_sequence": "MGQKVNSNGLRFGINKNWISRWTANSHAQTAKWLIEDEKIRNLFFVNYRNAQVSNVEIERTQATVDVFVYAAQPAFLIGSENKNIQKITKQIKQIIGRTTNLDLTINEIGSPMLSARIIARDLANAIEARVPLRTAMRQSLIKVLKAGANGIKVLVSGRLNGAEIARDKMYIEGNMPLSTLRADIDYALEKAQTTYGVIGVKVWINRGMIYTKGLNRTPAHILHPQKKQPNRQNQQPRHFNQGQVLSANKLTGSDVETSSIQALTKPNKEDKQ" }, "paralog": false, "identity": 0.9963369963369964, "score": 532.0, "evalue": 3.77e-194, "description": "Internal stop codon at 220076. Nonsense mutation around 220076.", "psc": { "uniref90_id": "UniRef90_P41205", "gene": "rpsC", "product": "30S ribosomal protein S3", "uniref50_id": "UniRef50_O52338", "cog_id": "COG0092", "cog_category": "J", "kegg_orthology_id": "K02982", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P41205", "UniRef:UniRef50_O52338", "COG:COG0092", "COG:J", "KEGG:K02982", "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52338" ], "product": "Small ribosomal subunit protein uS3" } }, "id": "GIHJHMOALN_422", "locus": "GCA009939745_00387" }, { "type": "cds", "sequence": "contig_1", "start": 220834, "stop": 221220, "strand": "+", "frame": 1, "gene": "rplP", "product": "50S ribosomal protein L16", "db_xrefs": [ "COG:COG0197", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02878", "SO:0001217", "UniRef:UniRef50_P02415", "UniRef:UniRef90_P47404" ], "nt": "ATGTTACAACCAAAAAGAACAAAGTACCGCAAACCTCACAACGTGAGTTACGAGGGCAAGGCTAAGGGCAACAGCTATGTTGCTTTTGGTGAATATGGTCTAGTAGCTACTAAGGGTAACTGGATCGATGCCCGTGCCATAGAATCAGCCCGGATTGCCATTAGTAAGTGTTTGGGTAAAACCGGTAAGATGTGGATCCGCATCTTCCCCCACATGTCCAAGACTAAAAAACCGTTAGAAGTACGGATGGGTTCTGGTAAGGGTAACCCGGAATTCTGGGTAGCAGTAGTAAAACAGGGCACGGTGATGTTTGAGGTAGCTAACATCCCTGAAAGCCAAATGATTAAGGCTTTAACCCGTGCTGGTCACAAGTTACCCGTGACCTGA", "aa": "MLQPKRTKYRKPHNVSYEGKAKGNSYVAFGEYGLVATKGNWIDARAIESARIAISKCLGKTGKMWIRIFPHMSKTKKPLEVRMGSGKGNPEFWVAVVKQGTVMFEVANIPESQMIKALTRAGHKLPVT", "aa_hexdigest": "a065ea1cc9e1d1d733ac3d40561c3b20", "start_type": "ATG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_P47404", "query_cov": 1.0, "subject_cov": 0.920863309352518, "identity": 1.0, "score": 262.0, "evalue": 1.23e-88, "valid": true, "gene": "rplP", "product": "50S ribosomal protein L16", "uniref50_id": "UniRef50_P02415", "cog_id": "COG0197", "cog_category": "J", "kegg_orthology_id": "K02878", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P02415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P02415" ], "product": "Large ribosomal subunit protein uL16" }, "genes": [ "rplP" ], "id": "GIHJHMOALN_423", "locus": "GCA009939745_00388" }, { "type": "cds", "sequence": "contig_1", "start": 221253, "stop": 221447, "strand": "+", "frame": 3, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "SO:0001217", "UniRef:UniRef50_Q50310", "UniRef:UniRef90_Q50310" ], "nt": "ATGACCGTTGCAAAAGAATTACGCCAAAAGAGTTCCGAAGAGTTGGTAAAACTCGTGATTAAGCTCAAAGGGGAACTGTTAGAGTACCGCTTTAAGTTAGCACACGGGGAGCTAGACAAACCGCACTTAATTAACCAAACCAGGCGGTTATTGGCTACCATTTTAACGATTCTTACCGAGCGTAAGTTGAACTGA", "aa": "MTVAKELRQKSSEELVKLVIKLKGELLEYRFKLAHGELDKPHLINQTRRLLATILTILTERKLN", "aa_hexdigest": "3c1ca8354d0101d23fdc06853121825e", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpmC" ], "truncated": "3-prime", "pseudogene": { "start": 221253, "stop": 221588, "observations": { "mutation": [ 221445 ], "stop": [ 221445 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50310", "query_cov": 1.0, "subject_cov": 0.5765765765765766, "identity": 1.0, "score": 119.0, "evalue": 9.11e-34, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MTVAKELRQKSSEELVKLVIKLKGELLEYRFKLAHGELDKPHLINQTRRLLATILTILTERKLNWQEEQAKYKLLTKKTNEAAVNAWKQHLEANKAKLLKSRAKREDASKK" }, "paralog": false, "identity": 0.990990990990991, "score": 210.0, "evalue": 5.6e-72, "description": "Internal stop codon at 221445. Nonsense mutation around 221445.", "psc": { "uniref90_id": "UniRef90_Q50310", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_Q50310", "cog_id": "COG0255", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50310", "UniRef:UniRef50_Q50310", "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q50310", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50310" ], "product": "Large ribosomal subunit protein uL29" } }, "id": "GIHJHMOALN_424", "locus": "GCA009939745_00389" }, { "type": "cds", "sequence": "contig_1", "start": 221588, "stop": 221845, "strand": "+", "frame": 2, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "COG:COG0186", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_014325389.1", "SO:0001217", "UniParc:UPI0002385E52", "UniRef:UniRef100_A0AB33HP98", "UniRef:UniRef50_P47406", "UniRef:UniRef90_Q50309" ], "nt": "ATGAAACGTAATCAAAGAAAGGTCCTAATTGGGATTGTTAAAAGTACTAAAAACGCCAAGACCGCTACGGTTCAAGTAGAATCACGGTTTAAGCACCCTTTGTACCATAAGTCCGTAGTGCGAAACAAGAAGTACCAAGCCCACAATGAGGGTGAAGTACTCGCTAAGGACGGGGACAAGGTACAAATTGTGGAAACGCGACCGTTGTCTGCAACCAAGCGCTTCCGCATTGCCAAAATTATTGAGAGGGCTAAATAG", "aa": "MKRNQRKVLIGIVKSTKNAKTATVQVESRFKHPLYHKSVVRNKKYQAHNEGEVLAKDGDKVQIVETRPLSATKRFRIAKIIERAK", "aa_hexdigest": "b9e5aedc166ca703c8cc756adedb0e22", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002385E52", "ncbi_nrp_id": "WP_014325389.1", "uniref100_id": "UniRef100_A0AB33HP98", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002385E52", "RefSeq:WP_014325389.1", "UniRef:UniRef100_A0AB33HP98" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HP98", "uniref90_id": "UniRef90_Q50309" }, "psc": { "uniref90_id": "UniRef90_Q50309", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_P47406", "cog_id": "COG0186", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47406" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "GIHJHMOALN_425", "locus": "GCA009939745_00390" }, { "type": "cds", "sequence": "contig_1", "start": 221861, "stop": 222217, "strand": "+", "frame": 2, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02874", "SO:0001217", "UniRef:UniRef50_Q5SHP8", "UniRef:UniRef90_P47407" ], "nt": "ATGACAAGACTAAATGTTGCCGATAACACGGGCGCCAAGCAAGTGGGCATCATTAAGGTATTAGGTTCCACCCGGAAACGTTATGCCTTTTTGGGTGATGTGGTAGTGGTATCAGTTAAGGATGCTATCCCTAGCGGTATGGTCAAAAAGGGTCAAGTGCTACGGGCAGTAATTGTCCGCACTAAAAAGGGCCAACAACGTAAGGATGGTACCCACCTTAAGTTTGATGACAACGCTTGTGTGTTAATTAAGGAAGACAAGTCACCAAGAGGCACACGGATCTTTGGTCCGGTAGCGCGTGAACTACGGGAACGTGGTTACAACAAAATTTTGAGTTTAGCAGTGGAGGTAGTGTAA", "aa": "MTRLNVADNTGAKQVGIIKVLGSTRKRYAFLGDVVVVSVKDAIPSGMVKKGQVLRAVIVRTKKGQQRKDGTHLKFDDNACVLIKEDKSPRGTRIFGPVARELRERGYNKILSLAVEVV", "aa_hexdigest": "1d4d34751b9f8d42d5606a6c0bb09451", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47407", "query_cov": 1.0, "subject_cov": 0.9672131147540983, "identity": 0.9490000000000001, "score": 220.0, "evalue": 1.37e-72, "valid": true, "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_Q5SHP8", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHP8" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "GIHJHMOALN_426", "locus": "GCA009939745_00391" }, { "type": "cds", "sequence": "contig_1", "start": 222217, "stop": 222552, "strand": "+", "frame": 1, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874533.1", "SO:0001217", "UniParc:UPI0000133DCD", "UniRef:UniRef100_Q50307", "UniRef:UniRef50_O52343", "UniRef:UniRef90_Q50307" ], "nt": "ATGCAACGAATTAAAAAAGGTGATAAAGTAGTCGTAATTACCGGTAAGAATAAGGGTGGTAGTGGCATTGTTTTGAAAATTATGCCCGCGCGCCAACAAGCAATTGTAGAGGGACTCAACAAAGTAACACGCCACAAAAAGAAGGACCAAACCACAAAGCGCGCTGCTAAACAATCGACCGGTAAAGTGCAACAAGAAGCGCCAATCTTCTTGAGCAAGTTAGCTTTATTTGACCAAAAGGCAAAACAACAAACAATTGGCAAAATTAAGTACGTTATGGACCCTAAGACTAATAAGAAGACCCGGGTCTTTAAGAAATCCAACAACACGCTCTAG", "aa": "MQRIKKGDKVVVITGKNKGGSGIVLKIMPARQQAIVEGLNKVTRHKKKDQTTKRAAKQSTGKVQQEAPIFLSKLALFDQKAKQQTIGKIKYVMDPKTNKKTRVFKKSNNTL", "aa_hexdigest": "b672114d9ef8bdd0628ab567967334f1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DCD", "ncbi_nrp_id": "WP_010874533.1", "uniref100_id": "UniRef100_Q50307", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DCD", "RefSeq:WP_010874533.1", "UniRef:UniRef100_Q50307" ] }, "ips": { "uniref100_id": "UniRef100_Q50307", "uniref90_id": "UniRef90_Q50307" }, "psc": { "uniref90_id": "UniRef90_Q50307", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_O52343", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_O52343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52343" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "GIHJHMOALN_427", "locus": "GCA009939745_00392" }, { "type": "cds", "sequence": "contig_1", "start": 222555, "stop": 223097, "strand": "+", "frame": 3, "gene": "rplE", "product": "50S ribosomal protein L5", "db_xrefs": [ "COG:COG0094", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02931", "RefSeq:WP_010874534.1", "SO:0001217", "UniParc:UPI0000134132", "UniRef:UniRef100_Q50306", "UniRef:UniRef50_Q50306", "UniRef:UniRef90_Q50306" ], "nt": "ATGAATAATCTCAAAGCACATTACCAAAAAACAATTGCCAAAGAGCTGCAAAAGAGCTTTGCGTTTTCCTCGATTATGCAAGTACCGCGCTTGGAAAAAATTGTGATTAACATGGGGGTGGGTGACGCCATTCGCGACAGTAAGTTCTTGGAATCAGCCTTAAACGAACTCCACCTCATTAGCGGTCAAAAACCAGTTGCTACTAAGGCTAAAAACGCCATTTCCACCTATAAGCTGCGCGCTGGTCAACTAATCGGTTGTAAGGTTACCTTGCGCGGTGAGCGGATGTGGGCCTTTTTGGAAAAACTGATTTATGTTGCCCTACCTCGGGTGCGGGATTTCCGCGGCCTTTCTTTAAAGTCTTTTGATGGTCGGGGTAACTATACCATTGGAATTAAAGAACAAATTATTTTCCCGGAAATTGTCTATGACGACATTAAACGGATCCGCGGGTTTGACGTTACTCTGGTAACTTCAACCAATAAGGACAGCGAAGCATTAGCTTTACTTAGAGCGTTAAACCTCCCACTTGTAAAAGGATAA", "aa": "MNNLKAHYQKTIAKELQKSFAFSSIMQVPRLEKIVINMGVGDAIRDSKFLESALNELHLISGQKPVATKAKNAISTYKLRAGQLIGCKVTLRGERMWAFLEKLIYVALPRVRDFRGLSLKSFDGRGNYTIGIKEQIIFPEIVYDDIKRIRGFDVTLVTSTNKDSEALALLRALNLPLVKG", "aa_hexdigest": "edbdda012ca37706b0304b21ef094906", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134132", "ncbi_nrp_id": "WP_010874534.1", "uniref100_id": "UniRef100_Q50306", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134132", "RefSeq:WP_010874534.1", "UniRef:UniRef100_Q50306" ] }, "ips": { "uniref100_id": "UniRef100_Q50306", "uniref90_id": "UniRef90_Q50306" }, "psc": { "uniref90_id": "UniRef90_Q50306", "gene": "rplE", "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_Q50306", "cog_id": "COG0094", "cog_category": "J", "kegg_orthology_id": "K02931", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q50306", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50306" ], "product": "Large ribosomal subunit protein uL5" }, "genes": [ "rplE" ], "id": "GIHJHMOALN_428", "locus": "GCA009939745_00393" }, { "type": "cds", "sequence": "contig_1", "start": 223099, "stop": 223281, "strand": "+", "frame": 1, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "COG:COG0199", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_P12878", "UniRef:UniRef90_P47410" ], "nt": "ATGGCCAAAAAATCATTAAAAGTAAAACAAACTAGAATCCCCAAATTTGCCGTACGGGCTTACACCCGCTGTCAGCGTTGTGGTCGAGCCCGGGCAGTTTTAAGCCACTTTGGTGTGTGCCGTTTGTGCTTCCGCGAACTAGCATACGCTGGCGCAATTCCAGGAGTTAAAAAAGCATCATGA", "aa": "MAKKSLKVKQTRIPKFAVRAYTRCQRCGRARAVLSHFGVCRLCFRELAYAGAIPGVKKAS", "aa_hexdigest": "e4be5aeb126691b9d760f7afa251758a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47410", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 0.9, "score": 103.0, "evalue": 3.34e-28, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_P12878", "cog_id": "COG0199", "cog_category": "J", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P12878", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P12878" ], "product": "Small ribosomal subunit protein uS14B" }, "genes": [ "rpsZ" ], "id": "GIHJHMOALN_429", "locus": "GCA009939745_00394" }, { "type": "cds", "sequence": "contig_1", "start": 223278, "stop": 223706, "strand": "+", "frame": 3, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874536.1", "SO:0001217", "UniParc:UPI000013516F", "UniRef:UniRef100_Q50304", "UniRef:UniRef50_Q9PQP6", "UniRef:UniRef90_Q50304" ], "nt": "ATGATAACAACTACAAAACCAATTAAAGCCCACTTTGATCCAGTGGCTGACTTACTAACGAAGATTAACAATGCGCGCAAAGCGAAGTTAATGACGGTAACCACAATTGCTTCCAAGCTCAAGATTGCCATCTTGGAAATTTTGGTCAAGGAAGGTTATTTAGCTAACTTCCAGGTCTTGGAAAACAAGAGCAAGACCAAGCGGATTGTCACCTTTAACTTAAAATACACGCAGCGCCGGATTCCCTCCATTAACGGGGTGAAACAAATTTCCAAGCCGGGTCTGCGGATCTACCGGCCCTTTGAAAAGCTCCCGTTAGTTTTAAACGGTCTTGGGATTGCCATTATTTCCACTAGTGATGGCGTGATGACTGATAAGGTAGCGCGCTTAAAGAAAATTGGAGGGGAGATTCTCGCTTACGTTTGGTAA", "aa": "MITTTKPIKAHFDPVADLLTKINNARKAKLMTVTTIASKLKIAILEILVKEGYLANFQVLENKSKTKRIVTFNLKYTQRRIPSINGVKQISKPGLRIYRPFEKLPLVLNGLGIAIISTSDGVMTDKVARLKKIGGEILAYVW", "aa_hexdigest": "7d47000330783529090b357afb242771", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013516F", "ncbi_nrp_id": "WP_010874536.1", "uniref100_id": "UniRef100_Q50304", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013516F", "RefSeq:WP_010874536.1", "UniRef:UniRef100_Q50304" ] }, "ips": { "uniref100_id": "UniRef100_Q50304", "uniref90_id": "UniRef90_Q50304" }, "psc": { "uniref90_id": "UniRef90_Q50304", "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q9PQP6", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP6" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "GIHJHMOALN_430", "locus": "GCA009939745_00395" }, { "type": "cds", "sequence": "contig_1", "start": 223713, "stop": 224174, "strand": "+", "frame": 3, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "SO:0001217", "UniRef:UniRef50_P47412", "UniRef:UniRef90_Q50303" ], "nt": "ATGTCAAAAATAGGAAACAGAACGATTACACTAGACCCCGCTAAGGTCAACCTTAACTTCCAGAAGGACCACATTGCCGTTAAGGGTCCTTTGGGTCAAATTGAGCTCAAACTACCCCCTAACTTACCGCTCAAGTTTGAACTGAAGGATAATAACCTCCAAATTACGCGGAACAACGAGTTAAAGCAATCCAAGATCTTCCATGGTACTTACAACGCGTTAATTACCAACGCCATTATTGGGGTTACTCAAGGCTTTGAAAAGAAGCTGCGCTTAGTCGGTGTGGGTTACCGTGCTAACGTGGAAGGGGAAACGCTCAACCTCCAGTTGGGTTACTCCCATCCCATTAAGGAAAAGATTCCCAAAGGCTTAACAGTTAAAGTAGAAAAGAACACCGAGATTACCATTTCGGGAATTAGTAAGGAGTTAGTGGGTCAGTTTGCCACTGAGGTGCGCAAGTGA", "aa": "MSKIGNRTITLDPAKVNLNFQKDHIAVKGPLGQIELKLPPNLPLKFELKDNNLQITRNNELKQSKIFHGTYNALITNAIIGVTQGFEKKLRLVGVGYRANVEGETLNLQLGYSHPIKEKIPKGLTVKVEKNTEITISGISKELVGQFATEVRK", "aa_hexdigest": "148b1fbb56239c07daf79d0ce4fc7212", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q50303", "query_cov": 1.0, "subject_cov": 0.8315217391304348, "identity": 1.0, "score": 302.0, "evalue": 2.22e-103, "valid": true, "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_P47412", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47412" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "GIHJHMOALN_431", "locus": "GCA009939745_00396" }, { "type": "cds", "sequence": "contig_1", "start": 224268, "stop": 224618, "strand": "+", "frame": 3, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "COG:COG0256", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625", "RefSeq:WP_010874538.1", "SO:0001217", "UniParc:UPI0000133C7F", "UniRef:UniRef100_Q50302", "UniRef:UniRef50_Q9PQP4", "UniRef:UniRef90_Q50302" ], "nt": "ATGAAAACACGAACAGAACAACGGCGCTTACGCCATAAAAGGATTGTGAAGAAGATCCGCGCCACTAACCACGACAACCGCGTTGTGTTAATGGTGATTAAGTCCTTAAACCATATTAGTGTGCAAGCGTGGGACTTTTCCCAGAACATTGTTTTAGCGTCCTCTTCCTCACTTGCTTTAAAATTAAAGAATGGTAACAAGGACAATGCCAAGTTAGTGGGGCAAGACATTGCTGATAAACTAGTGAAGCTCAAACTAACCAACGTTGTCTTTGACACTGGTGGTAGTAAGTACCACGGCCGGATTGCGGCATTGGCCGAAGCAGCCCGCGAAAGGGGGTTAAACTTCTAA", "aa": "MKTRTEQRRLRHKRIVKKIRATNHDNRVVLMVIKSLNHISVQAWDFSQNIVLASSSSLALKLKNGNKDNAKLVGQDIADKLVKLKLTNVVFDTGGSKYHGRIAALAEAARERGLNF", "aa_hexdigest": "32ec61768f43ea6307058b5153b62ce2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133C7F", "ncbi_nrp_id": "WP_010874538.1", "uniref100_id": "UniRef100_Q50302", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133C7F", "RefSeq:WP_010874538.1", "UniRef:UniRef100_Q50302" ] }, "ips": { "uniref100_id": "UniRef100_Q50302", "uniref90_id": "UniRef90_Q50302" }, "psc": { "uniref90_id": "UniRef90_Q50302", "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_Q9PQP4", "cog_id": "COG0256", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP4" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "GIHJHMOALN_432", "locus": "GCA009939745_00397" }, { "type": "cds", "sequence": "contig_1", "start": 224622, "stop": 225281, "strand": "+", "frame": 3, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "COG:COG0098", "COG:J", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02988", "RefSeq:WP_010874539.1", "SO:0001217", "UniParc:UPI0000135061", "UniRef:UniRef100_Q50301", "UniRef:UniRef50_Q50301", "UniRef:UniRef90_Q50301" ], "nt": "ATGACTGATCAAAACCAAAAAGCAAATCAAGGTAACGGCTTGCAAACCACTAACCTCCAAGCCCACGCACAGCGTAAACACAACCTCCGTCCTTCCTCTGAAGGGATTAAGAAAGCCGTGTCCAAAAAGGAAGGTGGTGGTCACAACCGTAACAACCAAAACCGTCGCTTCCAAAAACCGGCTTTCAAGAGTGAGTTTGAAGAACGCATCGTCAAACTAAAGCGCATTTCTAAAACCACTAAGGGTGGACGGAACATGCGGTTTAGTGTGCTGGTCGTAGTTGGTAACCGCAAGGGCAAGATCGGTTATGGGATTGCTAAGGCCTTGGAAGTACCGAATGCGATTAAGAAAGCCATTAAAGCAGCGCACAACTCACTCCACACAATTGAAATCCACAAGGGTTCAATTTACCACGAAGTAATTGGCCGTAGTGGGGCGAGTCGCGTGTTGCTCAAACCCGCTCCTCAAGGTACGGGAATTATTGCTGGTGGTGCCATTCGGGCCATTATTGAGCTCGCTGGTTACAGTGACATTTACACCAAGAACCTCGGTCGCAACACGCCAATTAACATGATCCATGCCACCATGGATGGGATCTTAAAGCAGCTCTCTCCACGTCGGGTAGCGATCTTACGTAACAAAAACCTCAACGAACTTTAG", "aa": "MTDQNQKANQGNGLQTTNLQAHAQRKHNLRPSSEGIKKAVSKKEGGGHNRNNQNRRFQKPAFKSEFEERIVKLKRISKTTKGGRNMRFSVLVVVGNRKGKIGYGIAKALEVPNAIKKAIKAAHNSLHTIEIHKGSIYHEVIGRSGASRVLLKPAPQGTGIIAGGAIRAIIELAGYSDIYTKNLGRNTPINMIHATMDGILKQLSPRRVAILRNKNLNEL", "aa_hexdigest": "02492a98fc2901ada19459181bae0900", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135061", "ncbi_nrp_id": "WP_010874539.1", "uniref100_id": "UniRef100_Q50301", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135061", "RefSeq:WP_010874539.1", "UniRef:UniRef100_Q50301" ] }, "ips": { "uniref100_id": "UniRef100_Q50301", "uniref90_id": "UniRef90_Q50301" }, "psc": { "uniref90_id": "UniRef90_Q50301", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_Q50301", "cog_id": "COG0098", "cog_category": "J", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q50301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50301" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "GIHJHMOALN_433", "locus": "GCA009939745_00398" }, { "type": "cds", "sequence": "contig_1", "start": 225285, "stop": 225740, "strand": "+", "frame": 3, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02876", "RefSeq:WP_014325390.1", "SO:0001217", "UniParc:UPI0001DD64E0", "UniRef:UniRef100_A0AAV5N9G4", "UniRef:UniRef50_P47415", "UniRef:UniRef90_Q50300" ], "nt": "ATGGAACTCAATCAACTCAAAAGTGTCCCCAAAGCGCGAAACCACAAGACCAAAACTTTAGGTCGTGGGCACGGCTCGGGTTTAGGTAAAACATCTGGTCGAGGCCAGAAGGGGCAAAAAGCGCGCAAGTCCGGTTTAACCCGTCCTGGTTTTGAAGGAGGACAAACGCCACTGTACCGTCGTTTACCGAAGTTTGGTAACGCACGCAAGGGCTTTTTAAAGCAGGAGTGGGTGGTGTTAAACCTCAATAAAATTGCTAAATTAAAGCTCGACAAAATTAACCGCGCTAGCTTAATTGAAAAGCAAGTAATTAGCGCAAAGAGCCAATTACCAATTAAACTAATTGGCAACACTAAGCTCGAAAAGCCGTTGCACTTTGAGGTGCACAAGGTTTCCAAGCAAGCTTTGAAAGCGGTTGAAAACGCCAACGGTAGTGTGAAGCTGTTAGAGAAATAA", "aa": "MELNQLKSVPKARNHKTKTLGRGHGSGLGKTSGRGQKGQKARKSGLTRPGFEGGQTPLYRRLPKFGNARKGFLKQEWVVLNLNKIAKLKLDKINRASLIEKQVISAKSQLPIKLIGNTKLEKPLHFEVHKVSKQALKAVENANGSVKLLEK", "aa_hexdigest": "de1dc562ff0880811058f25e3bf5aab2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64E0", "ncbi_nrp_id": "WP_014325390.1", "uniref100_id": "UniRef100_A0AAV5N9G4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64E0", "RefSeq:WP_014325390.1", "UniRef:UniRef100_A0AAV5N9G4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N9G4", "uniref90_id": "UniRef90_Q50300" }, "psc": { "uniref90_id": "UniRef90_Q50300", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_P47415", "cog_id": "COG0200", "cog_category": "J", "kegg_orthology_id": "K02876", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47415" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "GIHJHMOALN_434", "locus": "GCA009939745_00399" }, { "type": "cds", "sequence": "contig_1", "start": 225740, "stop": 227173, "strand": "+", "frame": 2, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0005886", "GO:0006605", "GO:0015031", "GO:0016020", "GO:0043952", "GO:0065002", "RefSeq:WP_014325391.1", "SO:0001217", "UniParc:UPI0001DD64E1", "UniRef:UniRef100_A0AAX0SR74", "UniRef:UniRef50_O52351", "UniRef:UniRef90_Q59548" ], "nt": "ATGCAAGCCAAACCAACAACTGCAGTTAAACAAAAACAGAATTTTGGGCAACGTTTGTTCACTTTGTTGCGCAACCGTGACTTCATGATCTCGTTTTTAATAACGGTCGTGCTGTTGGTTTTGTTTCGTGTTTTAGCGATTATTCCGCTCCCCGGCATCCAAGTCAACCAAACCGGGCTAGACCAAAACTCAAACGATTTCTTCTCACTGTTTAACCTACTTGGTGGGGGTGGATTGAACCAACTATCGCTGTTTGCGGTGGGAATTAGTCCGTATATTTCGGCGCAAATTATTATGCAGCTGTTGTCAACGGACTTAATTCCCCCGTTATCTAAACTGGTGAACAGTGGGGAAGTGGGTCGGCGTAAAATCGAGATGATTACGCGGATTATTACGCTACCGTTTGCGTTGGTGCAATCGTTTGCTGTGATCCAAATTGCCACTAATTCCGGTGGCGGTTCTTCACCTATTACCTTAAAAAACAACGGTTCGGATTTTGTCGCGTTTTACATCATTGCCATGACTGCTGGCACCTACCTGTCGGTCTTTTTAGGTGACACCATTTCCAAAAAGGGAATTGGTAACGGGATCACCCTGTTAATTCTTTCGGGGATTTTAGCCCAGCTACCCGAAGGATTTATAGCTGCTTACAGCGTGTTATCAGGTGTAGTGGTAACCATTAACGCAACCTTAACCACTGCCATTAGCTTCTTCATTTACTTCATGGCGTTTTTGGCATTACTGTTTGCCACTACCTTTATTACCCAAGCGACCCGGAAGATTCCGATTCAACAATCCGGTCAGGGTTTAGTAACCGAATCCTCTGCGTTACCTTACCTCCCAATTAAGGTCAACTCCGCCGGGGTAATTCCGGTGATCTTTGCGTCTTCGATTATGTCAATTCCGGTGACGATTGCCCAGTTCCAACCGCAAACCGAATCACGCTGGTTTGTGGAGGACTATCTTTCACTGTCCAAACCAACTGGCATTGTCCTGTATGGTATCTTGGTGATTCTCTTTAGCTTCTTTTACAGCTACATCCAGATCAATCCAGAACGGTTAGCCAAAAACTTCGAGAAGTCCGGGCGTTTTATCCCGGGTATTCGTCCCGGTAAGGATACTGAGAAACACATTGCCAGAGTACTCGTGCGGATTAACTTTATTGGCGCCCCTTTCCTAACGGTGATTGCCATTATTCCTTACATAGTGTCAGCCTTAATCCACTTACCCAACTCCTTAAGTTTAGGGGGTACGGGGATTATTATCATTGTTACCGCTGTGGTTGAGTTTATGTCAGCACTCCGCTCTGCAGCCACCGCTACTAACTACCAACAACTGCGCCGCAACTTGGCGATTGAAGTGCAAAAAACAGCCCAACAAGACAAGGAGGAACAACTGCGCGCTGAAACCCCTGGTATTGGTAACCTCTGGTAA", "aa": "MQAKPTTAVKQKQNFGQRLFTLLRNRDFMISFLITVVLLVLFRVLAIIPLPGIQVNQTGLDQNSNDFFSLFNLLGGGGLNQLSLFAVGISPYISAQIIMQLLSTDLIPPLSKLVNSGEVGRRKIEMITRIITLPFALVQSFAVIQIATNSGGGSSPITLKNNGSDFVAFYIIAMTAGTYLSVFLGDTISKKGIGNGITLLILSGILAQLPEGFIAAYSVLSGVVVTINATLTTAISFFIYFMAFLALLFATTFITQATRKIPIQQSGQGLVTESSALPYLPIKVNSAGVIPVIFASSIMSIPVTIAQFQPQTESRWFVEDYLSLSKPTGIVLYGILVILFSFFYSYIQINPERLAKNFEKSGRFIPGIRPGKDTEKHIARVLVRINFIGAPFLTVIAIIPYIVSALIHLPNSLSLGGTGIIIIVTAVVEFMSALRSAATATNYQQLRRNLAIEVQKTAQQDKEEQLRAETPGIGNLW", "aa_hexdigest": "2b796d7ff43988d8d6d129625471b5aa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64E1", "ncbi_nrp_id": "WP_014325391.1", "uniref100_id": "UniRef100_A0AAX0SR74", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64E1", "RefSeq:WP_014325391.1", "UniRef:UniRef100_A0AAX0SR74" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SR74", "uniref90_id": "UniRef90_Q59548" }, "psc": { "uniref90_id": "UniRef90_Q59548", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_O52351", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0006605", "GO:0015031", "GO:0016020", "GO:0043952", "GO:0065002" ] }, "pscc": { "uniref50_id": "UniRef50_O52351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52351" ], "product": "Protein translocase subunit SecY" }, "genes": [ "secY" ], "id": "GIHJHMOALN_435", "locus": "GCA009939745_00400" }, { "type": "cds", "sequence": "contig_1", "start": 227173, "stop": 227814, "strand": "+", "frame": 1, "gene": "adk", "product": "Adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0004017", "GO:0005524", "GO:0005737", "GO:0008270", "GO:0043231", "GO:0044209", "SO:0001217", "UniParc:UPI0001DD64E2", "UniRef:UniRef100_Q50299", "UniRef:UniRef50_Q50299", "UniRef:UniRef90_Q50299" ], "nt": "ATGGAAAATAAGTTCTTGTTTTTAGGCGCTCCGGGTGTGGGTAAGGGTACACTAGCTAAGCAAGTAGCCAACACCACCGGATTGTTTCACCTTTCCACTGGTGACATCTTTCGCAGTGTGATGCAAGAGCAGGGTGCGTTGAGTCAAACGCTAGCGCACTATATGAACCAGGGTTTGTATGTCCCTGATGAATTGACTAACCAAACCTTTTGGCACTTTGTCACTACCCACCAAAATGAACTCCACAAGGGCTTTATCCTAGATGGGTATCCCCGTACCTTAAACCAACTCGAGTTTTTGCAAAGCAAGTTGCAATTAGACCAGGTGTTTCACCTTAAGCTAAGTGATCCCCAAGTGCTAGTAGCGCGGATCTTAAACCGTTTGGTTTGTCCTAGTTGTGGTAGTGTATACAACAAGCAGAGCAAACCACCATTGAAGGCCAATCAGTGTGATCGTTGCCATGCTACGTTACAAGCGCGCAATGATGACACGGAAGCGGTGATCTTAAAGCGTTTAACGCTTTACGAAGACACCGTTAAACCCTTAATTGAGGCCTTCACCAAGCAGGGCATTCTCACCGTCATTGAAGCCCAATTACCGCTCGAACAACAGGTCAATTTGGTTCAACAGTTAGTGCATTAA", "aa": "MENKFLFLGAPGVGKGTLAKQVANTTGLFHLSTGDIFRSVMQEQGALSQTLAHYMNQGLYVPDELTNQTFWHFVTTHQNELHKGFILDGYPRTLNQLEFLQSKLQLDQVFHLKLSDPQVLVARILNRLVCPSCGSVYNKQSKPPLKANQCDRCHATLQARNDDTEAVILKRLTLYEDTVKPLIEAFTKQGILTVIEAQLPLEQQVNLVQQLVH", "aa_hexdigest": "fd4a177fd8fd988c3ebaacc33fb1ce32", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64E2", "uniref100_id": "UniRef100_Q50299", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64E2", "UniRef:UniRef100_Q50299" ] }, "ips": { "uniref100_id": "UniRef100_Q50299", "uniref90_id": "UniRef90_Q50299" }, "psc": { "uniref90_id": "UniRef90_Q50299", "gene": "adk", "product": "Adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q50299", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q50299", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50299" ], "product": "Adenylate kinase" }, "genes": [ "adk" ], "id": "GIHJHMOALN_436", "locus": "GCA009939745_00401" }, { "type": "cds", "sequence": "contig_1", "start": 227814, "stop": 228560, "strand": "+", "frame": 3, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006", "KEGG:K01265", "RefSeq:WP_010874543.1", "SO:0001217", "UniParc:UPI0000125A05", "UniRef:UniRef100_Q11132", "UniRef:UniRef50_P47418", "UniRef:UniRef90_Q11132" ], "nt": "ATGGTTTATTTAAAGTCTGCGCGTGAGGTCGAACAGATCCGCCAAGCATGTAAGATTTTCCAAGAAGCAAAGGCATATTTTACAATTGAAAGGCTACTTGGCAAATCTTTAACAGCAATTGATCAAGCGTTAAAGCAGTTTATTGAGAGCAAAGGTGCAACTTGTGCCTTTCATAAATACCAAAATTTCCCGGGATTTAACTGCCTTTCGTTAAACGAAACGGTAATCCATGGCATAGCGGACAACCGTGTTTTTGGTGTTAAGGATAAACTGACGTTGGATATTGGCATTAACCTTAACGGTTATATTTGCGATGCAGCTTTTACGGTTTTGGGCCCCAAGGCTCCAGAGCCCATGCAAACGCTGTTAGAGGTAACGGAAGCTTGTTTTACTGCTGTGGTGGAGCCTCAACTGCGCCCCAATAACCCCACTGGTAATGTTTCCCATGCCATCCAAACCTACTTTGAATCTAAGGGTTATTACCTTTTAAAACAGTTTGGTGGTCATGGTTGTGGGATTAAGGTGCACGAAGAACCGCTGATTTTGAACTATGGTAAGCCTGATACAGGTACTAAGTTAGAACCAGGGATGGTCTTGTGCATTGAGCCCATGGTAATGACTGACAGTGATGCAATGGTAATGCATAACAACAGCTGGAATGTATTAACCCCTAAAAGCCGTTACAATTGTCATGTAGAACAGATGTATGTGATCACCACAAGTGGATTTGAATGTTTAACTAATTAA", "aa": "MVYLKSAREVEQIRQACKIFQEAKAYFTIERLLGKSLTAIDQALKQFIESKGATCAFHKYQNFPGFNCLSLNETVIHGIADNRVFGVKDKLTLDIGINLNGYICDAAFTVLGPKAPEPMQTLLEVTEACFTAVVEPQLRPNNPTGNVSHAIQTYFESKGYYLLKQFGGHGCGIKVHEEPLILNYGKPDTGTKLEPGMVLCIEPMVMTDSDAMVMHNNSWNVLTPKSRYNCHVEQMYVITTSGFECLTN", "aa_hexdigest": "2f1b193bf84ffb3adf81d5e2d92c6692", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125A05", "ncbi_nrp_id": "WP_010874543.1", "uniref100_id": "UniRef100_Q11132", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125A05", "RefSeq:WP_010874543.1", "UniRef:UniRef100_Q11132" ] }, "ips": { "uniref100_id": "UniRef100_Q11132", "uniref90_id": "UniRef90_Q11132" }, "psc": { "uniref90_id": "UniRef90_Q11132", "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_P47418", "cog_id": "COG0024", "cog_category": "J", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_P47418", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47418" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "GIHJHMOALN_437", "locus": "GCA009939745_00402" }, { "type": "cds", "sequence": "contig_1", "start": 228560, "stop": 228796, "strand": "+", "frame": 2, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022", "KEGG:K02518", "RefSeq:WP_010874544.1", "SO:0001217", "UniParc:UPI000012D263", "UniRef:UniRef100_Q50298", "UniRef:UniRef50_Q50298", "UniRef:UniRef90_Q50298" ], "nt": "ATGCAACCAAAGTTTAATAACCAAGCAAAGCAGGACAAACTCGTCTTAACGGGCAAAATCTTGGAAATCATCCACGGTGACAAGTTTCGCGTGTTGTTGGAAAACAACGTTGAAGTAGATGCTCACTTAGCGGGCAAAATGCGAATGCGCCGGTTACGAATTTTACCGGGCGATTTAGTTGAAGTGGAATTTTCCCCTTATGATTTAAAGTTAGGTCGAATAATAGGTAGAAAATAA", "aa": "MQPKFNNQAKQDKLVLTGKILEIIHGDKFRVLLENNVEVDAHLAGKMRMRRLRILPGDLVEVEFSPYDLKLGRIIGRK", "aa_hexdigest": "95747ef8ca2e0f64bb414f5618436f45", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012D263", "ncbi_nrp_id": "WP_010874544.1", "uniref100_id": "UniRef100_Q50298", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D263", "RefSeq:WP_010874544.1", "UniRef:UniRef100_Q50298" ] }, "ips": { "uniref100_id": "UniRef100_Q50298", "uniref90_id": "UniRef90_Q50298" }, "psc": { "uniref90_id": "UniRef90_Q50298", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q50298", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q50298", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50298" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "GIHJHMOALN_438", "locus": "GCA009939745_00403" }, { "type": "cds", "sequence": "contig_1", "start": 228920, "stop": 229177, "strand": "+", "frame": 2, "gene": null, "product": "30S ribosomal protein S13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "nt": "ATGGCACGGATTTTAGGTATTGACATTCCCAACCAGAAACGCATAGAAATTGCGTTAACCTATATTTTTGGTATAGGGCTTTCGCGCTCCCAAGCCATCTTAAAGCAAGCGAACATTAACCCCGATAAACGGGTCAAAGACTTAACCGAAGAGGAGTTTGTTGCCATTAGGAATGTAGCTAGTGCTTACAAGATTGAGGGTGATTTGCGTCGTGAAATTGCACTCAACATTAAGCATTTGAGTGAAATTGGAGCATGA", "aa": "MARILGIDIPNQKRIEIALTYIFGIGLSRSQAILKQANINPDKRVKDLTEEEFVAIRNVASAYKIEGDLRREIALNIKHLSEIGA", "aa_hexdigest": "7d2df25be7fa43522d3bb68be647b043", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031B5D25D", "query_cov": 1.0, "subject_cov": 0.9770114942528736, "identity": 0.565, "score": 95.5, "evalue": 4.27e-24, "valid": false, "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_A0A535XWH1", "kegg_orthology_id": "K02952" }, "pscc": { "uniref50_id": "UniRef50_A0A535XWH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "product": "30S ribosomal protein S13" }, "genes": [], "id": "GIHJHMOALN_439", "locus": "GCA009939745_00404" }, { "type": "cds", "sequence": "contig_1", "start": 229294, "stop": 229404, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAGAAGAAAAAGATTAATGTATCTAGTGGAATTATCCACGTATCCTGTTCCCCAAATAACACAATTGTATCCGCTAGTGATCCGGGGGGCAACGTGTTGTGCTGA", "aa": "MAKKKKINVSSGIIHVSCSPNNTIVSASDPGGNVLC", "aa_hexdigest": "6fa3ed66a08dcd96c26be2d606006744", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3670.243200000001, "isoelectric_point": 9.303384208679198 }, "id": "GIHJHMOALN_440", "locus": "GCA009939745_00405" }, { "type": "cds", "sequence": "contig_1", "start": 229420, "stop": 229659, "strand": "+", "frame": 1, "gene": "rpsK", "product": "30S ribosomal protein S11", "db_xrefs": [ "COG:COG0100", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02948", "SO:0001217", "UniRef:UniRef50_Q50296", "UniRef:UniRef90_Q50296" ], "nt": "ATGGGCTTTAAGGGATCGCGCAAAAAGACCCCGTACTCAGCCGGCATAGCAGCCGATAAGGTGGCCAAAACCGTCAAGGAAATGGGGATGGCGACCGTTAAGCTCTTTGTCAAGGGTACAGGTCGGGGTAAGGATACCGCCATTCGCAGCTTTGCTAATGCGGGACTTTCTATTACTGAAATTAACGAAAAGACCCCGATTCCCCACAACGGTTGCAAACCACCAAAACGACCACGCTAA", "aa": "MGFKGSRKKTPYSAGIAADKVAKTVKEMGMATVKLFVKGTGRGKDTAIRSFANAGLSITEINEKTPIPHNGCKPPKRPR", "aa_hexdigest": "7f2dd1aa2c4078964274d355c8fc4a0b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A7Z0ENF1", "query_cov": 1.0, "subject_cov": 0.8061224489795918, "identity": 0.544, "score": 94.7, "evalue": 9.23e-24, "valid": false, "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_A0A2H5B013", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "genes": [ "rpsK" ], "truncated": "5-prime", "pseudogene": { "start": 229294, "stop": 229659, "observations": { "mutation": [ 229402 ], "stop": [ 229402 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50296", "query_cov": 1.0, "subject_cov": 0.6528925619834711, "identity": 1.0, "score": 161.0, "evalue": 8.04e-50, "gene_start": 1, "gene_end": 79, "reference_start": 43, "reference_end": 121, "reference_sequence": "MAKKKKINVSSGIIHVSCSPNNTIVSASDPGGNVLCWASSGTMGFKGSRKKTPYSAGIAADKVAKTVKEMGMATVKLFVKGTGRGKDTAIRSFANAGLSITEINEKTPIPHNGCKPPKRPR" }, "paralog": false, "identity": 0.9917355371900827, "score": 241.0, "evalue": 9.45e-84, "description": "Internal stop codon at 229402. Nonsense mutation around 229402.", "psc": { "uniref90_id": "UniRef90_Q50296", "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_Q50296", "cog_id": "COG0100", "cog_category": "J", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50296", "UniRef:UniRef50_Q50296", "COG:COG0100", "COG:J", "KEGG:K02948", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q50296", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50296" ], "product": "Small ribosomal subunit protein uS11" } }, "id": "GIHJHMOALN_441", "locus": "GCA009939745_00406" }, { "type": "cds", "sequence": "contig_1", "start": 229665, "stop": 229991, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01193.29" ], "nt": "ATGGAAAAGTTTTTAAAGTACGAAATAAAGGTAAATAACGAACAAGCACGCGCTAACCCCAACTACGGCATCTTTGAAGTTGGACCACTCGAATCGGGCTTTGGCATTACCATCGGTAATGCTATGCGCCGGGTGCTGTTGAGCTGTATTCCAGGTGCCAGTGTGTTTGCACTCTCAATTTCGGGTGCCAAGCAAGAGTTTGCTGCTGTGGAAGGGATGAAGGAAGATGTCACTGAAGTGGTGTTAAACTTCAAGCAATTGGTAGTGAAGATTTCTGACCTTCTGTTTGAAGATGGTGAAATGGTCGAACCACCACTAGAACGTTGA", "aa": "MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFGITIGNAMRRVLLSCIPGASVFALSISGAKQEFAAVEGMKEDVTEVVLNFKQLVVKISDLLFEDGEMVEPPLER", "aa_hexdigest": "a567bbe0c202bfbdc5329a463bb79ed1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01193.29", "name": "RNA_pol_L", "length": 64, "aa_cov": 0.5740740740740741, "hmm_cov": 0.765625, "evalue": 1.7386825237633103e-10, "score": 35.988704681396484, "start": 29, "stop": 90 } ], "seq_stats": { "molecular_weight": 11923.654199999995, "isoelectric_point": 4.7362455368042 }, "id": "GIHJHMOALN_442", "locus": "GCA009939745_00407" }, { "type": "cds", "sequence": "contig_1", "start": 229995, "stop": 230648, "strand": "+", "frame": 3, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_P47423", "UniRef:UniRef90_Q50295" ], "nt": "TTGTTAACGGTAACAGCTGAGAAAGCGGGACCGGTGTATGCGAAGGACTTGGAGTGCCCAGCTGGCTTTGAAGTGGTCAACAAGGACTTGTACCTGTTCTCGTTGCAAACTGACAAAAAGGTAACAGTTAACGTGTATGTGAAACAAGGACGGGGGTTTGTTACTTTCTTGGAAAACCGTGAAATGATTAATTCTTTGGGGATTATTGCCACTGACTCGAACTTCTCCCCGGTATTACACTGTGGTTATGAAGTACAGGAATTGAAAACTTCCAAGCAAAAGATTACTGATCACTTAACTTTCAAAATTGCTACTAACGGTGCCATATCCGCTGTGGATGCCTTTGCAATGGCTGCAAAGATCTTGATCGAACACCTCAATCCGATTGTTAATGTCAACGAATCGATTAAGGCGTTGAATATTATCCAAGAGAAAGCCGAAGAACGCCGTGTCCGTTCGTTTGCGAAGCAAATTGAAGAGTTGGACTTTACCGTGCGTACCTTTAACTGTTTGAAGCGTAGTGGGATCCACACCTTACAAGAGTTGTTGTCCAAGTCATTAGCTGATATTCGTGAAATCCGTAACCTCGGTAAGAAATCGGAACGCGAAATTATTAAGAAGGTACACGAATTAGGACTCAAATTAAGATCTTAG", "aa": "MLTVTAEKAGPVYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQGRGFVTFLENREMINSLGIIATDSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIVNVNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIREIRNLGKKSEREIIKKVHELGLKLRS", "aa_hexdigest": "cbf888f5bf323cea9f11ef7210ca5bfd", "start_type": "TTG", "rbs_motif": null, "genes": [ "rpoA" ], "truncated": "5-prime", "pseudogene": { "start": 229695, "stop": 230648, "observations": { "mutation": [ 229989 ], "stop": [ 229989 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50295", "query_cov": 1.0, "subject_cov": 0.6636085626911316, "identity": 0.995, "score": 414.0, "evalue": 1.49e-144, "gene_start": 1, "gene_end": 217, "reference_start": 111, "reference_end": 327, "reference_sequence": "MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFVITIGNAMRRVLLSCIPGASVFALSISGAKQEFAAVEGMKEDVTEVVLNFKQLVVKISDLLFEDGEMVEPPLERWPLLTVTAEKAGPVYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQGRGFVTFLENREMINSLGIIATDSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIVNVNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIREIRNLGKKSEREIIKKVHELGLKLRS" }, "paralog": false, "identity": 0.9936908517350158, "score": 607.0, "evalue": 1.11e-222, "description": "Internal stop codon at 229989. Nonsense mutation around 229989.", "psc": { "uniref90_id": "UniRef90_Q50295", "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47423", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50295", "EC:2.7.7.6", "UniRef:UniRef50_P47423", "COG:COG0202", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P47423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47423" ], "product": "DNA-directed RNA polymerase subunit alpha" } }, "id": "GIHJHMOALN_443", "locus": "GCA009939745_00408" }, { "type": "cds", "sequence": "contig_1", "start": 230696, "stop": 231025, "strand": "+", "frame": 2, "gene": "rplQ", "product": "50S ribosomal protein L17", "db_xrefs": [ "COG:COG0203", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02879", "SO:0001217", "UniRef:UniRef50_P47424", "UniRef:UniRef90_Q59547" ], "nt": "ATGACCGTGCGCCAACAGGTAAGCGCTGTTTTGGCTTATGGCAAGATTGAAACAACCTTAAAGAAGGCCAAGAACACCCAAAAGCGTTTAGATAAGTTAATTACCTTAGCTAAAGTTGATAACTTTAATAACCGTCGTCAAGTCAAAAAGTGGTTGTTAAACACCAACTTGTTCGATGTTGATCAACTAATGGATCACTTGTTTAGCAAAGTTGCCCCTAAATACGAAAAAACCCCCGGTGGTTACAGCCGTGTTTTAAAATTAGGGCCACGTCGCGGAGATGCCACGGAAATGGCCATCTTGCAACTAACTGATGCTAAATACAAATAA", "aa": "MTVRQQVSAVLAYGKIETTLKKAKNTQKRLDKLITLAKVDNFNNRRQVKKWLLNTNLFDVDQLMDHLFSKVAPKYEKTPGGYSRVLKLGPRRGDATEMAILQLTDAKYK", "aa_hexdigest": "58f9dbd146e31873ccf00c49f2709be5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q59547", "query_cov": 1.0, "subject_cov": 0.8790322580645161, "identity": 1.0, "score": 215.0, "evalue": 1.38e-70, "valid": true, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P47424", "cog_id": "COG0203", "cog_category": "J", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47424" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [ "rplQ" ], "id": "GIHJHMOALN_444", "locus": "GCA009939745_00409" }, { "type": "cds", "sequence": "contig_1", "start": 231009, "stop": 231824, "strand": "+", "frame": 3, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:7.-.-.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47425", "UniRef:UniRef90_Q50294" ], "nt": "ATGCTAAATACAAATAAACAAGCAGCTGCAAGCTTTCAAGGTGTTTATTTTTCTTACAGCGAATTACCATTAATTCGCAACCTCTCCTTTGATGTTTTCGAAGGGGAGTATGTTTGCATAGTTGGCCATAACGGCTCGGGTAAATCCACCATCTCGAAGCTGTTAACGGGCTTGTTAAAACCACAAGCGGGTACCATCAAAATCTTTGGTAAGACGATTTCGAGTGAAAACGTGACTTACCTACGCAACCACATTGGCATTATCTTCCAAAACCCCGATAACCAGTTTATTGGTATTACCGTGGAAGATGACATTGCTTTTGGACTGGAAAACCGCCGTTATACGCGCGAAAAGATGAAGAGCATCATTGAGGATGTAGCCCAACAAGCGGGAATAACGGAGTTATTGCAAAAAGAACCGCATAACCTTTCGGGTGGGCAAAAGCAACGCGTTGCCATTGCCTCGGTGTTAGCCTTAAACCCAGCCATCATTATCTTTGATGAGTCGACTTCGATGTTGGATCCTAAAGCGAAGCGTACCATTAAGCAGTTCATGGTGGAACTGCGCAACCAGGGCAAGTGTGTTATCTCCATTACCCATGACATGGAAGAGGTTACCAAAGCAGACAAGGTTTTGGTCATGAATGAAGGTCGTTTAATTCGTCAAGGTAAACCCAAGGATGTCTTTAACAGTGCAGCCGAATTGCAAAAAATCCGCTTGGATATTCCCTTTTCTTTAAGCCTTTCCACGAAGGTTAATGGTGTGACTAGTACAATAGACTACCAACAACTAATTGAGGCCATTGGTAAGCTATGA", "aa": "MLNTNKQAAASFQGVYFSYSELPLIRNLSFDVFEGEYVCIVGHNGSGKSTISKLLTGLLKPQAGTIKIFGKTISSENVTYLRNHIGIIFQNPDNQFIGITVEDDIAFGLENRRYTREKMKSIIEDVAQQAGITELLQKEPHNLSGGQKQRVAIASVLALNPAIIIFDESTSMLDPKAKRTIKQFMVELRNQGKCVISITHDMEEVTKADKVLVMNEGRLIRQGKPKDVFNSAAELQKIRLDIPFSLSLSTKVNGVTSTIDYQQLIEAIGKL", "aa_hexdigest": "3c727d3ba5186aa4c199538b80687fe0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q50294", "query_cov": 1.0, "subject_cov": 0.9890510948905109, "identity": 1.0, "score": 525.0, "evalue": 5.27e-188, "valid": true, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "7.-.-.-" ], "uniref50_id": "UniRef50_P47425", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_P47425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47425" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [ "ecfA1" ], "id": "GIHJHMOALN_445", "locus": "GCA009939745_00410" }, { "type": "cds", "sequence": "contig_1", "start": 231821, "stop": 232732, "strand": "+", "frame": 2, "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:3.6.3.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_010874551.1", "SO:0001217", "UniParc:UPI00001393F6", "UniRef:UniRef100_Q50293", "UniRef:UniRef50_Q50293", "UniRef:UniRef90_Q50293" ], "nt": "ATGAAAAAAATAGTTGAACTCAAAAACCCCTTAAGGGATGGAGAAATCCTCAGTGTTAGTAACCTTTCGTGCTTTTTTAACGAAAAAACAGTCCATGAAGTCAAGGTAATTGACAACTTTAGCTATACCTTTGCTAAAAATAAGGTCTACTGCATTATTGGTGACAGTGGTAGTGGTAAATCAACCTTAGTCAACCATTTTAATGGTTTAATAAAGCCCGCTTACGGAGATATTTGGGTCAAGGATATTTACATTGGTCAAAAGCAACGCAAAATTAAGGACTTTAAACGCTTGCGCAAAACAATTTCGATTGTCTTTCAGTTTCCCGAATACCAACTGTTTAAGGACACAGTGGAAAAGGACATTATGTTCGGTCCAATTGCTTTGGGTCAATCGAAAAACGAAGCACGCCAAAAGGCAGCTTACTACCTCGAAAAAATGGGGTTAAAGTACACTTTTTTAGAGCGCAATCCGTTTGAGTTGAGCGGGGGGCAAAAGCGCCGTGTAGCGATTGCTGGGATTTTAGCAATTGAACCCGAAGTTTTAATCTTTGATGAACCTACCGCTGGACTAGATCCTGAAGGGGAACGAGAAATGATGCGTTTAATTAAGGATGCTAAAGCTAGTGGTCGCACCGTCTTTATGATTACCCACCAAATGGAAAATGTTTTGGAAGTAGCGGATGAAGTGCTGGTGTTGTCCAAGGGCCAACTCGTTAAATCCGGTGATCCTTACGAAGTTTTTATGGATGAAGCATTTTTGGCACACACCACCATTATTATGCCTCCAGTCATTCAAGTTATCAAAGACCTAATTAACTTAAACCCGAAGTTTAGTTGTTTATTAGACTTTAAACCGCGCAACTTAGACCAGTTGGCGGATGCCATTAACAACACAATAGCTCATGGATAG", "aa": "MKKIVELKNPLRDGEILSVSNLSCFFNEKTVHEVKVIDNFSYTFAKNKVYCIIGDSGSGKSTLVNHFNGLIKPAYGDIWVKDIYIGQKQRKIKDFKRLRKTISIVFQFPEYQLFKDTVEKDIMFGPIALGQSKNEARQKAAYYLEKMGLKYTFLERNPFELSGGQKRRVAIAGILAIEPEVLIFDEPTAGLDPEGEREMMRLIKDAKASGRTVFMITHQMENVLEVADEVLVLSKGQLVKSGDPYEVFMDEAFLAHTTIIMPPVIQVIKDLINLNPKFSCLLDFKPRNLDQLADAINNTIAHG", "aa_hexdigest": "6d515d943aee2ee12155c598a96e60ca", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001393F6", "ncbi_nrp_id": "WP_010874551.1", "uniref100_id": "UniRef100_Q50293", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393F6", "RefSeq:WP_010874551.1", "UniRef:UniRef100_Q50293" ] }, "ips": { "uniref100_id": "UniRef100_Q50293", "uniref90_id": "UniRef90_Q50293" }, "psc": { "uniref90_id": "UniRef90_Q50293", "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "3.6.3.-" ], "uniref50_id": "UniRef50_Q50293", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_Q50293", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50293" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" }, "genes": [ "ecfA2" ], "id": "GIHJHMOALN_446", "locus": "GCA009939745_00411" }, { "type": "cds", "sequence": "contig_1", "start": 232725, "stop": 233066, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGTTTTTTTATTAATGGTTATGTACCACGAGATACCTTTATCCACCGGCTCCACCCGACAACGAAACTGTTAATCTTTTTGCTGTTCGTGATCCTGGTGTTTGTACCCATTGGCTTTGTTTTTCAAAGTGTGATCTTTGTCTTTGCCACAGTTATTTTCTTTGTAGCCAAACTACCCGGACGGTTTTACCTCTCCTCGATTAAGTCAATCTCACTGTTATTCTTACTACTGTTATTTGTCAACTGGTTTACCTTCCGTGACCCCGGGTTCTACATTACCGCTGATCAGGTTAATACAGTAAAACCACACTTTAACGGTAATAACTTTAACTTCTGA", "aa": "MDSFFINGYVPRDTFIHRLHPTTKLLIFLLFVILVFVPIGFVFQSVIFVFATVIFFVAKLPGRFYLSSIKSISLLFLLLLFVNWFTFRDPGFYITADQVNTVKPHFNGNNFNF", "aa_hexdigest": "db2dfa0461e906685a91c52d88b2aa7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13196.49359999998, "isoelectric_point": 9.868062782287598 }, "id": "GIHJHMOALN_447", "locus": "GCA009939745_00412" }, { "type": "cds", "sequence": "contig_1", "start": 233097, "stop": 233570, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTCGCAAGTGTTTAACTTTAACCGTGCCAACATGACAGAGTTAAACAAGATTAACTTCTTCTTTAAGGAAACAGCTAATGCTAATGCATACACCAAGGTAACGGGGATTGATAAATTGGCAGAAATGTTGGCTTCAAAGAATCTCTTTAAGTTCAACGGTTCTACCAATGGCATTGATAAAAACAAGATTCTTGGTGCCTTTCTAGATCATAAGATTGCCGTTTACCTCGGTCGTAGTTGGGGTGGTGACTTTAGTGGTTTTGTGATTGACGTAAGTGTAAGTGATAAAACATCTACCTTCACGATTAAACCCTTTTTAGCTAACTCGAACTATGTGTTGACACTGCGTGCCATTATTCTAGCCTTTTACGTTACCCAAAAGATCTTGATCATGATCATTTTGGCAACAGTGTTAACTTCCACTTCAAGCTCGGTTGAGTTGGCATACGGAATTGAACGCTTGCTGTGA", "aa": "MFSQVFNFNRANMTELNKINFFFKETANANAYTKVTGIDKLAEMLASKNLFKFNGSTNGIDKNKILGAFLDHKIAVYLGRSWGGDFSGFVIDVSVSDKTSTFTIKPFLANSNYVLTLRAIILAFYVTQKILIMIILATVLTSTSSSVELAYGIERLL", "aa_hexdigest": "15aba9bee48ef64822d4ee107cc53f6f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17461.085499999997, "isoelectric_point": 9.486925697326662 }, "id": "GIHJHMOALN_448", "locus": "GCA009939745_00413" }, { "type": "cds", "sequence": "contig_1", "start": 233574, "stop": 234029, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGCTGTTACGGGTTCCAGTCAATGTGTTTGCGATGACAATTGCAATTGCAATTCGCTTTGTCCCTTCTTTATTGTTGGAATCACAACGGATTTTAAATGCCCAAGCATCACGCGGTTTGGACTTTAAGAACGGCAATTTCTTTGTCAAAATGCGTTCATTGTCTTCCTTGGTTGTACCGATGATTTCGATTGCATTCCGCAATGCTGGGGAGTTGGCGAGTGCCATGGAAGCACGTGGTTATGATCCAACTAAGAAACGTACTACCTACCGCAAGTTTAAGATTGATTGGGTTGATGCCACAGCTTTAATTCTCACCGCTCTTTACTTTGTGGTGATTATTTTCTTAACCGTTAAGGGTGCAGTGTTCCTAGATTTAGGGACACCTGAGTGGTTGCTAACCGGCAAGATTAAAGAGCAGGTAGAGCGCTCATTATCAGTTAAAAGTGCCTAG", "aa": "MKLLRVPVNVFAMTIAIAIRFVPSLLLESQRILNAQASRGLDFKNGNFFVKMRSLSSLVVPMISIAFRNAGELASAMEARGYDPTKKRTTYRKFKIDWVDATALILTALYFVVIIFLTVKGAVFLDLGTPEWLLTGKIKEQVERSLSVKSA", "aa_hexdigest": "092427315b7d96369286f153f0d841c0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16885.89759999999, "isoelectric_point": 10.232824897766111 }, "id": "GIHJHMOALN_449", "locus": "GCA009939745_00414" }, { "type": "cds", "sequence": "contig_1", "start": 234184, "stop": 234405, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATGCCATTAACCAAACCTTTCACGTTGATCTACCTGATAAGTTACCACTCAAACAGTTAATTGCCAAACTAAACCAATTAAACTACCCTGACATTTTTGTAAAGGCCTTAGTTCCAGTAGCACCGCGATTTCACGCGCGGTTTGCAGTAAAAACTAAGGTTTATGAATACCTTATTAACACCCGAGCTTTTAACCCAGCACAGTACCACACCGTGTGA", "aa": "MHAINQTFHVDLPDKLPLKQLIAKLNQLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQYHTV", "aa_hexdigest": "0b4ed275fcc0223680c33b317db5effd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8488.865399999997, "isoelectric_point": 9.820742988586424 }, "id": "GIHJHMOALN_450", "locus": "GCA009939745_00415" }, { "type": "cds", "sequence": "contig_1", "start": 234421, "stop": 234753, "strand": "+", "frame": 1, "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "db_xrefs": [ "COG:COG0101", "COG:J", "EC:5.4.99.12", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147", "SO:0001217", "UniRef:UniRef50_P47428", "UniRef:UniRef90_Q50291" ], "nt": "TTGGATTTAGCACAACTTGAAGCGGATACCACGCTCTTTTTAGGCAGGAAGGATTTTTGGAGTTTTAGCACCGCTAGCCAAAAGGACACGCAGCGCACAATTAGTAAAATTATCGTGCAACAAGATCACACTGGTACGGTTAAACTGACCTTTTTTGGCAACGGTTTTTTAAGAAACCAGATTCGAATGATGGTAGCTTCTCTAGTGGCCTTAAACAATCGAACCCTGATGCGAGAAACAGTTCAACAGCTGTTTGATCAACCACAAAAGGGTGGGTGTAAGGTTAAAGCCCCCGCTTGTGGTCTTTATTTAAAAACTGTCTTATATGAATAA", "aa": "MDLAQLEADTTLFLGRKDFWSFSTASQKDTQRTISKIIVQQDHTGTVKLTFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTVLYE", "aa_hexdigest": "f5672fd11b674d80eb5d9d5e690c2004", "start_type": "TTG", "rbs_motif": null, "genes": [ "truA" ], "truncated": "5-prime", "pseudogene": { "start": 234121, "stop": 234753, "observations": { "mutation": [ 234403 ], "stop": [ 234403 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50291", "query_cov": 1.0, "subject_cov": 0.45267489711934156, "identity": 0.991, "score": 220.0, "evalue": 7.31e-71, "gene_start": 1, "gene_end": 110, "reference_start": 134, "reference_end": 243, "reference_sequence": "MPRYLAIVAYNGAPFRGWAKQPGYHTVQGQIEQNLALIYQTPINIYGSGRTDKGVHAINQTFHVDLPDKLPLKQLIAKLNQLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQYHTVWQYNHPLDLAQLEADTTLFLGRKDFWSFSTASQKDTQRTISKIIVQQDHTGTVKLTFFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKVKAPACGLYLKTVLYE" }, "paralog": false, "identity": 0.9952380952380953, "score": 421.0, "evalue": 1.39e-152, "description": "Internal stop codon at 234403. Nonsense mutation around 234403.", "psc": { "uniref90_id": "UniRef90_Q50291", "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "ec_ids": [ "5.4.99.12" ], "uniref50_id": "UniRef50_P47428", "cog_id": "COG0101", "cog_category": "J", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50291", "EC:5.4.99.12", "UniRef:UniRef50_P47428", "COG:COG0101", "COG:J", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147" ] }, "pscc": { "uniref50_id": "UniRef50_P47428", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47428" ], "product": "tRNA pseudouridine synthase A" } }, "id": "GIHJHMOALN_451", "locus": "GCA009939745_00416" }, { "type": "cds", "sequence": "contig_1", "start": 234950, "stop": 235147, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTAACAAGCACGCTGAAAACAGTTTGGACAAAACGATTAACGACGCTTTATTTCAGTACGAAATGTTTAAAAGTGAACATGCCTTGGTGTTTGTTGACTTTGAAAAGAACCTGTTTAAGCACGAAAAGGTGATTCGCGCATACCTCCAAGACCCTGCTTTAAAACAATACCAAAGGGATTTTGAACTAGTATGA", "aa": "MSNKHAENSLDKTINDALFQYEMFKSEHALVFVDFEKNLFKHEKVIRAYLQDPALKQYQRDFELV", "aa_hexdigest": "e91f966ec6cab7e0ec89fe83b197b7ff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7822.796200000001, "isoelectric_point": 5.83767147064209 }, "id": "GIHJHMOALN_452", "locus": "GCA009939745_00417" }, { "type": "cds", "sequence": "contig_1", "start": 235187, "stop": 235390, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGCGCAAATTAGTCCGGCATTAAACCAAGCGGACAAGATCTTTAATGTGTTATCTACCGCTGACCTAAACCTCCAACCAGTCGTATACAAGGGTAAAACTTACGTTATTAACGCGGTTAGTGATTATCAAAGTCTCTTGGAAAATAAGGATCGTGGCCTGCGTGAAGCTGCTTACAAAGTGTGGTTGGAAATTTACTGA", "aa": "MLAQISPALNQADKIFNVLSTADLNLQPVVYKGKTYVINAVSDYQSLLENKDRGLREAAYKVWLEIY", "aa_hexdigest": "96c7581ad1c48d323b02b29c4bc4a772", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7604.645400000003, "isoelectric_point": 6.080260276794433 }, "id": "GIHJHMOALN_453", "locus": "GCA009939745_00418" }, { "type": "cds", "sequence": "contig_1", "start": 235412, "stop": 235768, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTTTAGTGGAAAACTACATCCAATTAGAAACCTTTGCTAAGTTAAAGAAACACCCCAATTACATTGCCAAAACAGCTTTTGATGATGAAATTGATGTGGCTTTCATTGACTTTGTTTATGAGCAAGTGGCTAGCTTTGCCCCGACCTTTAAAGCCTTTCAAAGTTTAAGGAAGCAAATTTACAAGCACGTTTTAAAACTAGACAAAGCCCAACCCTGGGACTTGTCGGTACCGTTGTTTAAGGCTAGTGGTGACTACACCATTGAACAAGCCCAAACTGATGCGCTTAAGATATTAGCACCTATGGGTAGTGAATACCTGGAAGTGGTAAAGGAAGCCTTTCGGGAGCGCTGA", "aa": "MSLVENYIQLETFAKLKKHPNYIAKTAFDDEIDVAFIDFVYEQVASFAPTFKAFQSLRKQIYKHVLKLDKAQPWDLSVPLFKASGDYTIEQAQTDALKILAPMGSEYLEVVKEAFRER", "aa_hexdigest": "32012e5235e7afde35b71028d3387805", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13640.518099999988, "isoelectric_point": 5.671246910095214 }, "id": "GIHJHMOALN_454", "locus": "GCA009939745_00419" }, { "type": "cds", "sequence": "contig_1", "start": 235853, "stop": 236500, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTGATAAAACCCGCGCTTCATTAAATACCCTAGTCCATGAATTGGGTCACAGTGTCCATTCTTGGTATGCATCTAAGTACCAAACGCAAAACTTGGATCCTACAATTTTCTATGCTGAAATTGCTTCCATTTGTAACGAATTACTGTTGTGTTACCACGACATTATTAACTATGAAAACCGCAATCCGCAGCAGTTAATCCGTAGTTTAATGGAACAAATTAGCCACTTCTTTGGCGCTACCACGAGACAGTTAATGTTCTCTCAGTTTGAACAAGACACCTTAAAGTTAATTCAGCAAAACCAAAAGCCTGACTTTAAGACCCTAGTGGAAATTTATGGTAAGACCGCCATTAAGTACCAAGCTGCTAATGCTGACGCTATTACGAAAAAGCTCAAGCAAACCAAGTACCAAAAGTCATTGGCTTACATTACCTCCATTCCGCATTTTTACGCTGGTAACTTCTATGTTTACAAGTACGCCATTGGTCAAGTTGTGGGTACCTTAGTTGGTAAAAAACTGAGTGCTGGTGACAGTAATATGCTCGCTGCTTACAAGCGCTTCTTGTCTTCAGGCTCCACCTTACCACCACTAGAAACAATTAAATTGTTAGGGATTGACCTTACCCAACCAGAACCGTGA", "aa": "MNFDKTRASLNTLVHELGHSVHSWYASKYQTQNLDPTIFYAEIASICNELLLCYHDIINYENRNPQQLIRSLMEQISHFFGATTRQLMFSQFEQDTLKLIQQNQKPDFKTLVEIYGKTAIKYQAANADAITKKLKQTKYQKSLAYITSIPHFYAGNFYVYKYAIGQVVGTLVGKKLSAGDSNMLAAYKRFLSSGSTLPPLETIKLLGIDLTQPEP", "aa_hexdigest": "9b815534645efd0e635c0cdcc39bc895", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24490.7961, "isoelectric_point": 9.026621437072755 }, "id": "GIHJHMOALN_455", "locus": "GCA009939745_00420" }, { "type": "cds", "sequence": "contig_1", "start": 236581, "stop": 237060, "strand": "+", "frame": 1, "gene": "mte1", "product": "Type II methyltransferase M-MpnI", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0032259", "SO:0001217", "UniRef:UniRef50_Q50290", "UniRef:UniRef90_Q50290" ], "nt": "GTGCATTACTTTAACCGCGCCAAAAAGGCAAAGAACAATGAATTTTATACCTTGTTTGAAGACATTGCCGCTGAAGTGGCATGTTATCCCAATGCCTTTAAGGGTAAGGTAGTGTTGTGTAATTGTAATGATGGTTACCAATCGAACTTCTGGCAGTTTTTTCAAAGTCAGTTTCATGCTTTAGGACTCAAAAAACTAGTTGCGATTGCTTTTAATCCACTTGGCAACAGTTATCAACTCAACTTCGATGGAAAGGAAATAAAGGAACTACCTTTAGCTGGTAATGGCAGCTTTGACAGTGCGGAAGCCATTGTTCTTTTAAAGCAAAGTGACATTGTGGTAACTAATCCACCGTTTAGCTTGTTTCAGGACTTTGTTTGTCTTTTAGCAGAGCATGGCAAACAGTTTTTGGTTTTAGGTCACAATGGTGCTGTCGGTTATAATCAGATCTTTAAGCTGTTTAAGGAAGAACAGTTGTGA", "aa": "MHYFNRAKKAKNNEFYTLFEDIAAEVACYPNAFKGKVVLCNCNDGYQSNFWQFFQSQFHALGLKKLVAIAFNPLGNSYQLNFDGKEIKELPLAGNGSFDSAEAIVLLKQSDIVVTNPPFSLFQDFVCLLAEHGKQFLVLGHNGAVGYNQIFKLFKEEQL", "aa_hexdigest": "53cfc47731aef104e2fba8fda9c411f3", "start_type": "GTG", "rbs_motif": null, "genes": [ "mte1" ], "truncated": "3-prime", "pseudogene": { "start": 236581, "stop": 237363, "observations": { "mutation": [ 237058, 237223, 237328 ], "stop": [ 237058, 237223, 237328 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50290", "query_cov": 1.0, "subject_cov": 0.49843260188087773, "identity": 1.0, "score": 330.0, "evalue": 1.56e-112, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MHYFNRAKKAKNNEFYTLFEDIAAEVACYPNAFKGKVVLCNCNDGYQSNFWQFFQSQFHALGLKKLVAIAFNPLGNSYQLNFDGKEIKELPLAGNGSFDSAEAIVLLKQSDIVVTNPPFSLFQDFVCLLAEHGKQFLVLGHNGAVGYNQIFKLFKEEQLWYGHTVNSSMLFQVQSNFKLYDPKSVNFVKKDGQLFQKVPGISWFTNLKKNQQPAWLKTKSRYQGNEHKYPKFDWYDAIFVSKVKEIPLDWFGYMGVPLTFLNCFNPKQFELIDCLANPYATLDTLKTNAYVRSHHGDVRNVKGKRRYVRVVIKQRQNVI" }, "paralog": false, "identity": 0.9846153846153847, "score": 528.0, "evalue": 1.41e-192, "description": "Internal stop codon at 237058, 237223, 237328. Nonsense mutation around 237058, 237223, 237328.", "psc": { "uniref90_id": "UniRef90_Q50290", "gene": "mte1", "product": "Type II methyltransferase M.MpnI", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_Q50290", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50290", "EC:2.1.1.72", "UniRef:UniRef50_Q50290", "GO:0003677", "GO:0009007", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_Q50290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50290" ], "product": "Type II methyltransferase M.MpnI" } }, "id": "GIHJHMOALN_456", "locus": "GCA009939745_00421" }, { "type": "cds", "sequence": "contig_1", "start": 237085, "stop": 237225, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTCCAAGTGCAGTCTAACTTTAAACTGTATGACCCCAAAAGCGTTAACTTTGTTAAAAAGGATGGTCAACTGTTTCAAAAAGTACCGGGAATTTCCTGGTTTACAAACCTCAAGAAAAACCAACAACCAGCTTGA", "aa": "MLFQVQSNFKLYDPKSVNFVKKDGQLFQKVPGISWFTNLKKNQQPA", "aa_hexdigest": "04aa19e2bef512210a762ba521dde680", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5388.203800000001, "isoelectric_point": 10.000222969055176 }, "id": "GIHJHMOALN_457", "locus": "GCA009939745_00422" }, { "type": "cds", "sequence": "contig_1", "start": 237340, "stop": 237540, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGGTGCCATTAACCTTTCTCAATTGCTTTAATCCCAAACAGTTTGAGCTAATTGATTGTCTAGCCAATCCCTACGCTACCCTAGACACATTAAAAACTAATGCTTATGTGAGATCACACCACGGTGATGTGAGGAATGTGAAGGGTAAGCGAAGGTATGTTAGGGTAGTGATTAAACAACGTCAAAATGTGATTTAA", "aa": "MGVPLTFLNCFNPKQFELIDCLANPYATLDTLKTNAYVRSHHGDVRNVKGKRRYVRVVIKQRQNVI", "aa_hexdigest": "741a38653363bee739e4e54424ad7a9f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7660.864300000004, "isoelectric_point": 10.024140739440917 }, "id": "GIHJHMOALN_458", "locus": "GCA009939745_00423" }, { "type": "cds", "sequence": "contig_1", "start": 237634, "stop": 238428, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTCAAAATATTTTTGGGCAGCAGCTTTTTTGGTGTTGCCACCTTACTTAGTGCTTGTGGTACTGGTCGCTTTGATCAAATCGATGATGGCAAAATTAAGCTTGCTGTTGTCACATCCCCTAGTGCCGCTAGTTCACTGCAAACCTTATTAGATCAATACAACAACACTAAAGCACCAGCTGATTATCCAGTGGAAGTAGTTTCACTAGACAGTACTGGGAGCTATACTAAAGGGAAATTTGATACCCAAAAACGGTTAGCTGCTAAAGATAAAAATAACTTTTATAACCTAACCTTTAACTACCCCGACTTAGTTTCATCTTTAGCAATTAACGGAATGGAACTCAACCTTGATGGGGTTAATGTTTCTAACTTCGAACAGTCTTTCTTAGACTTCAACAAGAACATTAGTGGCGTACGCAAACCCGGCATTTACGCCTTACCAGCTACCATGAGTGGTGAAGTTTTGGTTTTAAATGGGCCAGTTCTCCACTACATCTTGAACAGTGCCAAGAAGAAGGATGGTACCGAATCAAAAATTAAAACTGCTCAATTAAAAACAGCTTCAACTCAGGCAGAAGTTAAGGGTACTATGACCATGGCTAGTGATGAACAAACCACAAAGCTTTGGACTAATATTCAAAACGCCGCTAAGGAAAATGCCGATAACGGTACAACTGAGAAAGCGGAAAAAACAGTTTCCGCTAGTTCGTTGCAATTAAAAAATACGAATAAAACCACCGAAGGTACCCTAAAAATTGGTACTGATGACAACACTAAAAATCTTTGA", "aa": "MKFKIFLGSSFFGVATLLSACGTGRFDQIDDGKIKLAVVTSPSAASSLQTLLDQYNNTKAPADYPVEVVSLDSTGSYTKGKFDTQKRLAAKDKNNFYNLTFNYPDLVSSLAINGMELNLDGVNVSNFEQSFLDFNKNISGVRKPGIYALPATMSGEVLVLNGPVLHYILNSAKKKDGTESKIKTAQLKTASTQAEVKGTMTMASDEQTTKLWTNIQNAAKENADNGTTEKAEKTVSASSLQLKNTNKTTEGTLKIGTDDNTKNL", "aa_hexdigest": "42ada610b3fb073b460af693806b07d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28508.791700000016, "isoelectric_point": 8.71536808013916 }, "id": "GIHJHMOALN_459", "locus": "GCA009939745_00424" }, { "type": "cds", "sequence": "contig_1", "start": 238831, "stop": 239649, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAACATTAAAAAAGGTCGCGCTGATTTTAGTAACTTCTTTAACCACAATTCAAAAACTTTCCAAAGTCTAAGAGATATTTTCAACAGCTTTAAGCCTTTAATTGACCAAAAGGGTTTAATTAATAACAAACACTTTGATACTCCAGTGAATAATTACGCTAAATTTCACCAATTGGCATTCTATGTATCATCGACAGCTCGCTTTCCTTACTCGTTTGCCAAAGACAATGTAAAACGCTTAATTATTGGTAAACGCGAATTGGAAGTAAACCCTAAATCAATGTTTGCCATTAAGAAGGAAAACGGCAATAACGGTAACAGCAACCTCTTAGGTAAAGTTGCACTAGATAACAATAAATCTATTGAACTTTACGAAAATAATATTCCTAACGGTAAAACTGATGCCATCTTAATTAAGAACCAAACTTTAATTAGTGCGTTGAAGAATCCAAAACAGACAAAGAGCTCTCAAAGATCTTTTCAGTCTACAAACACCCAAAATGATGCCATTTGTTACTTAGCCTTCAACAGCAAGATTAGACCAGACGATAAAGACATTTTCCTATTAGACAAATTTGGTGAAAAATTTGTCACCGCAATTGTTAACTTTGAAGAAAAAACGGAGGTCAAAATTAATACGCTCCAAGAAAAGGAAGCTGTTGTTTTACCAGCACCACAAAAGTTTAAACCTACTGATCCAAAATCAGTGGCACTTGTTCAAGGTCCAAGTTTAATCGGTATTCACGCTAACGCTAACGAAGACCGTTCAACCATTAAGTTTCTCAACTGGTACCTTAATGCTGAAGTTGATTGA", "aa": "MFNIKKGRADFSNFFNHNSKTFQSLRDIFNSFKPLIDQKGLINNKHFDTPVNNYAKFHQLAFYVSSTARFPYSFAKDNVKRLIIGKRELEVNPKSMFAIKKENGNNGNSNLLGKVALDNNKSIELYENNIPNGKTDAILIKNQTLISALKNPKQTKSSQRSFQSTNTQNDAICYLAFNSKIRPDDKDIFLLDKFGEKFVTAIVNFEEKTEVKINTLQEKEAVVLPAPQKFKPTDPKSVALVQGPSLIGIHANANEDRSTIKFLNWYLNAEVD", "aa_hexdigest": "1787dfbfd99d9e969212ed5d37e6f945", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30901.769800000024, "isoelectric_point": 9.520384788513184 }, "id": "GIHJHMOALN_460", "locus": "GCA009939745_00425" }, { "type": "cds", "sequence": "contig_1", "start": 239707, "stop": 240069, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGTTTAAAAAAGTATTAGAAAAATCCAAGCAAAACATAAAAGCGACCTCTAAGGAAGGAGAGCAAAATCAAGGCAAGAAAGGTGATGGTGCACAGAACCAAGGTAAGAAGGGCGATGGAGCTCAAAATGGTAAGAATGATAAAGCAAAGCACAATGGTTTTGTCGATATTGCCGTCAGCCAATTTTTAAACGGTTCTGTCGCTAAGTTTGTGGAACCAGCTGACTTTTTAGGTATTCGCTTACGTGATGCCGTTAAGAGTACCTTTAACGCTGCCATTAACAACACCTCAATTAACTTTGACAAGTTCATTCAGTACATTAACGATTCTTTAGGTAGCGACTTTAAGCAAAGACAGTAG", "aa": "MPFKKVLEKSKQNIKATSKEGEQNQGKKGDGAQNQGKKGDGAQNGKNDKAKHNGFVDIAVSQFLNGSVAKFVEPADFLGIRLRDAVKSTFNAAINNTSINFDKFIQYINDSLGSDFKQRQ", "aa_hexdigest": "7483ec5773002d78fc25461d4bb3f3d0", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13166.572299999983, "isoelectric_point": 9.697608375549319 }, "id": "GIHJHMOALN_461", "locus": "GCA009939745_00426" }, { "type": "cds", "sequence": "contig_1", "start": 240199, "stop": 240999, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTAAGTATGGTGCCATTGTTTTCAGTGGTCTTTTAGGAGTCTCTGCCATTTTAGCTGCTTGTGGTACACGCGGTAAATTTGACCAAATTGATGATGGCAAAATTAAGCTAGCATCTTCGTTAACTTCCAAAGGTGCCGCTAATGCTTTACAAGCAATTGTGAAGAAGTATAACGAGGTTAAAAAGCCTGGTGATTACCCAATTGAAATAACCCAAATTGCGGGTGGTTACGACCAAGCACGCGTTGACTTACAATCACGGGTTGGTGTTAAGGATAAAACCAATTTTTACAACCTCATTTTGAACTATCCCGATGTAGTTTCAGTGCTAGCACGTAACCAAATGGAACTGCCATTTGATGGTGTTGATGTTTCTAAAATTTCACCTAACTTTTTAAATTTTAACGAACGTATTAGCGGAGTTTCTAAAAAGGCTAACTATGCCATTCCGGTCTCTGTTTCGACTGATATTTTAGTGCTCAATGGACCAGTTTTGCACTACATTTTAAACAGTGCTAAAGGTGAAAGTAAGGGCGCACAAAAAGATAACAAGAGTGCTGAAGTTCAAAGAAAAAGTACAGGTCAAAAGACCGTTACCCAACCTTTAACTATTGCAACTGATTCTGCTACAAACGGTCTTTGGAAAAAGATTGAAGATGCTGCAAAAGTCAACGGTAAAAAGAAAGAAGAGAAAAAGAGTACACGCAGTAAAAGAGCCACAGAAGGCACACAAACCACCAAAGAAAATACGGGTGGTGATGCTGCAACAAGTGATACCAAAATTAAAGAATCTTGA", "aa": "MKFKYGAIVFSGLLGVSAILAACGTRGKFDQIDDGKIKLASSLTSKGAANALQAIVKKYNEVKKPGDYPIEITQIAGGYDQARVDLQSRVGVKDKTNFYNLILNYPDVVSVLARNQMELPFDGVDVSKISPNFLNFNERISGVSKKANYAIPVSVSTDILVLNGPVLHYILNSAKGESKGAQKDNKSAEVQRKSTGQKTVTQPLTIATDSATNGLWKKIEDAAKVNGKKKEEKKSTRSKRATEGTQTTKENTGGDAATSDTKIKES", "aa_hexdigest": "3720b466520c4994075f19e1a0f1c1e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28665.232800000027, "isoelectric_point": 9.57415142059326 }, "id": "GIHJHMOALN_462", "locus": "GCA009939745_00427" }, { "type": "cds", "sequence": "contig_1", "start": 241159, "stop": 242253, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_Q50288" ], "nt": "GTGTTAGGTGTTGATAATTCACCTAATGCTTTACTATCTTCGGTGTTTGCTGCTGGTAACAGTGACTATAACAACTTCTTTTATAAGGTGCAAAATGGTCGGGCTGACTTTAGCAACTTTAACAATAAAGGCAGTTCTTATCAAAACCTTAAAAATGTCTTTAACGACTATAAGAACCTAATTGCACAAAATGGTCTTTATGTAAATAAAGGCGGCTCTTACTCTTCAAACTTCCAAAAGTTTCACCAGTTAGCTTACAGCATTTCCTCTACTTCTGGTTTTGCTTATTCGTTTGCTGGACAAAATTCCAAACGTTTTAAATTTACTGATGATGGTACTTTTGTTGAGTATCCTAGTTATACAACTGAAGTTAATGCACCAGAAAGTAATAATGGCAATGATGGAAAGCAACAAGGTCAAAGTGATCAAGGAAACCTTTTAGGAACTTTTGAAGTTGTAGATAAAAGTACTAGTGATATAAAGGTGAAACCTAAAACTCAAGCGGAATCAAAGAAAAGTAGTGATAGTAAACAAACTGCAAACACAGGCAAAGGCAGTAATTCAAAACAACAAACTCCGAAGAAAACTATTTCGCTTTATAAAACCAAAATTCCTCAAGATAAAACAGAAAACGTTGACGCTTTTTTAGTTACTGATTCAGAATTAATTAGCAAACTTGAAAAAGCTAAAAATAAAAAGGAAGAAACTAAAGCTTCAGGTAAATCTGCATCTGCACGTGTGGCAGTTCAAGCAACTCAAAAGAAACAGTCTAACGAAAAGCAGATAGTTGGTTACACTACTACATCCGCATTAAGCGAAGACGGCAAACACATTTTTAAATTGGGTAAACTCAACAGCGAGAACTATGAGCGCAAAATCATTGTTGGTGCTACTGTAGAAACACTAGAACAATCCACTACCTTGCAAAGTGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAGTACAAGGATAGTGATCAAAAGAGAGTTATGATCACCCAAGGTCCTAACCTAATAGGGGTTCATGCTAATACTAAAGAAAATGAAGAGACTAAGAAATTTGTTAACTGGTTTTTAAATAAAACCGAAAGTTGA", "aa": "MLGVDNSPNALLSSVFAAGNSDYNNFFYKVQNGRADFSNFNNKGSSYQNLKNVFNDYKNLIAQNGLYVNKGGSYSSNFQKFHQLAYSISSTSGFAYSFAGQNSKRFKFTDDGTFVEYPSYTTEVNAPESNNGNDGKQQGQSDQGNLLGTFEVVDKSTSDIKVKPKTQAESKKSSDSKQTANTGKGSNSKQQTPKKTISLYKTKIPQDKTENVDAFLVTDSELISKLEKAKNKKEETKASGKSASARVAVQATQKKQSNEKQIVGYTTTSALSEDGKHIFKLGKLNSENYERKIIVGATVETLEQSTTLQSEEAIVLAAPGKYKDSDQKRVMITQGPNLIGVHANTKENEETKKFVNWFLNKTES", "aa_hexdigest": "7c378696e96dc07d36c06ef8d4558876", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 240859, "stop": 242556, "observations": { "mutation": [ 240997, 241066, 242251 ], "stop": [ 240997, 241066, 242251 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50288", "query_cov": 1.0, "subject_cov": 0.45614035087719296, "identity": 0.995, "score": 677.0, "evalue": 9.39e-239, "gene_start": 1, "gene_end": 364, "reference_start": 321, "reference_end": 684, "reference_sequence": "MKFKYGAIVFSGLLGVSAILAACGTRGKFDQIDDGKIKLASSLTSKGAANALQAIVKKYNEVKKPGDYPIEITQIAGGYDQARVDLQSRVGVKDKTNFYNLILNYPDVVSVLARNQMELPFDGVDVSKISPNFLNFNERISGVSKKANYAIPVSVSTDILVLNGPVLHYILNSAKGESKGAQKDNKSAEVQRKSTGQKTVTQPLTIATDSATNGLWKKIEDAAKVNGKKKEEKKSTRSKRATEGTQTTKENTGGDAATSDTKIKESWGAYQEVEGGLKGYQFKAIVFENWHDLIDFSTRVAKSFSKVKDNSNKKGNEIQGVLGVDNSPNALLSSVFAAGNSDYNNFFYKVQNGRADFSNFNNKGSSYKNLKNVFNDYKNLIAQNGLYVNKGGSYSSNFQKFHQLAYSISSTSGFAYSFAGQNSKRFKFTDDGTFVEYPSYTTEVNAPESNNGNDGKQQGQSDQGNLLGTFEVVDKSTSDIKVKPKTQAESKKSSDSKQTANTGKGSNSKQQTPKKTISLYKTKIPQDKTENVDAFLVTDSELISKLEKAKNKKEETKASGKSASARVAVQATQKKQSNEKQIVGYTTTSALSEDGKHIFKLGKLNSENYERKIIVGATVETLEQSTTLQSEEAIVLAAPGKYKDSDQKRVMITQGPNLIGVHANTKENEETKKFVNWFLNKTESWEVKGNGKDSQTTKSLTPAQYFAESASYILPLKETVEKDHKEQTNKNTYVAKALEMLKEVSENKSVSYSDPSDFRSGRFRDALGANFNATINSKANFEKFFQGFKAALGSDFDK" }, "paralog": false, "identity": 0.9929203539823008, "score": 1098.0, "evalue": 0.0, "description": "Internal stop codon at 240997, 241066, 242251. Nonsense mutation around 240997, 241066, 242251.", "psc": { "uniref90_id": "UniRef90_Q50288", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50288", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "GIHJHMOALN_463", "locus": "GCA009939745_00428" }, { "type": "cds", "sequence": "contig_1", "start": 242338, "stop": 242595, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGCTAAAGGAAACTGTTGAAAAAGATCATAAAGAACAAACTAATAAAAACACCTATGTTGCCAAAGCTTTAGAAATGCTCAAGGAAGTTAGTGAAAACAAATCAGTCAGCTATAGTGATCCGAGTGATTTTAGAAGTGGTAGATTCCGTGATGCACTTGGTGCTAACTTTAATGCCACTATTAATTCAAAAGCAAATTTTGAAAAGTTTTTCCAAGGTTTTAAAGCTGCATTAGGCTCTGATTTTGATAAATAG", "aa": "MPLKETVEKDHKEQTNKNTYVAKALEMLKEVSENKSVSYSDPSDFRSGRFRDALGANFNATINSKANFEKFFQGFKAALGSDFDK", "aa_hexdigest": "aca2633dd13680634e95ce37c493b8f6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9616.597199999993, "isoelectric_point": 7.948774528503418 }, "id": "GIHJHMOALN_464", "locus": "GCA009939745_00429" }, { "type": "cds", "sequence": "contig_1", "start": 242700, "stop": 242879, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGAGATTAAAACTTACAAAATCAAAGATATTTGTGATATTACACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGA", "aa": "MEIKTYKIKDICDITRGRVISKLDIKKDPGVFPVYSAATNNDGEFGRINSYDFDGEYVT", "aa_hexdigest": "5e30dbd3b8714ccd24b8094a741d69d4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 61, "aa_cov": 0.9661016949152542, "hmm_cov": 0.9836065573770492, "evalue": 1.6724861096036106e-10, "score": 36.86839294433594, "start": 3, "stop": 59 } ], "seq_stats": { "molecular_weight": 6708.495900000002, "isoelectric_point": 5.161514854431151 }, "id": "GIHJHMOALN_465", "locus": "GCA009939745_00430" }, { "type": "cds", "sequence": "contig_1", "start": 242860, "stop": 242955, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGA", "aa": "MVNMLLEQLTVMEGLFFIVMVNLASQIFVVF", "aa_hexdigest": "4165f2c157215872847f65036bab0f87", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3577.3857, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_466", "locus": "GCA009939745_00431" }, { "type": "cds", "sequence": "contig_1", "start": 243069, "stop": 243410, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HM83", "UniRef:UniRef90_A0AB33HM83", "PFAM:PF01420.25" ], "nt": "ATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGGGCGCGCAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTTAATCAAGAAAATATCCGAAAAATTTATGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACACATTAAAGATTGCGACTTTGATGGTGAATACATCACTTGA", "aa": "MAEIPIDFPPLKIQEKIATILDTFTELRARKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGHIKDCDFDGEYIT", "aa_hexdigest": "9e0fab2716bb870ba94d3a5e0a852fcd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 243099, "stop": 243713, "observations": { "mutation": [ 243408 ], "stop": [ 243408 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HM83", "query_cov": 0.9823008849557522, "subject_cov": 0.45491803278688525, "identity": 0.883, "score": 197.0, "evalue": 7.77e-62, "gene_start": 11, "gene_end": 113, "reference_start": 1, "reference_end": 111, "reference_sequence": "MQYQEKIATILDTFTELSAELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYITWTTNGYAGVVFYRNGKFNASQDCGVLKVQNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.9245283018867925, "score": 389.0, "evalue": 1.4e-139, "description": "Internal stop codon at 243408. Nonsense mutation around 243408.", "psc": { "uniref90_id": "UniRef90_A0AB33HM83", "uniref50_id": "UniRef50_A0AB33HM83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HM83", "UniRef:UniRef50_A0AB33HM83" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HM83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HM83" ] } }, "hypothetical": true, "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 62, "aa_cov": 0.5132743362831859, "hmm_cov": 0.9838709677419355, "evalue": 4.1016514491996766e-18, "score": 61.630435943603516, "start": 56, "stop": 113 } ], "seq_stats": { "molecular_weight": 13108.738399999984, "isoelectric_point": 5.838808250427246 }, "id": "GIHJHMOALN_467", "locus": "GCA009939745_00432" }, { "type": "cds", "sequence": "contig_1", "start": 243597, "stop": 243764, "strand": "+", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00190F3D87", "UniRef:UniRef90_UPI00190F3D87" ], "nt": "ATGGCTGAAATCGAACTCTCTTTCCCACCTCTAGAAATCCAAGAAAAGATTGCCGATATTCTCTTTGCTTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGA", "aa": "MAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "bbbc1991845ad67ac2a240d1c026608d", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 243450, "stop": 243809, "observations": { "mutation": [ 243762, 243783 ], "stop": [ 243762, 243783 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00190F3D87", "query_cov": 1.0, "subject_cov": 0.46218487394957986, "identity": 1.0, "score": 114.0, "evalue": 1.06e-31, "gene_start": 1, "gene_end": 55, "reference_start": 50, "reference_end": 104, "reference_sequence": "GKCNASQDCGVLKVKNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.8907563025210085, "score": 207.0, "evalue": 8.7e-71, "description": "Internal stop codon at 243762, 243783. Nonsense mutation around 243762, 243783.", "psc": { "uniref90_id": "UniRef90_UPI00190F3D87", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00190F3D87", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00190F3D87", "EC:3.1.21.3", "UniRef:UniRef50_UPI00190F3D87", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00190F3D87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00190F3D87" ], "product": "restriction endonuclease subunit S" } }, "id": "GIHJHMOALN_468", "locus": "GCA009939745_00433" }, { "type": "cds", "sequence": "contig_1", "start": 243815, "stop": 244240, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_308010181.1", "SO:0001217", "UniParc:UPI0027E159D2", "UniRef:UniRef100_UPI0027E159D2", "UniRef:UniRef50_A0AB38W7Y3", "UniRef:UniRef90_UPI0027E159D2" ], "nt": "GTGGTTTTCGTTGCCTTGGGTGTTATTCCCAGTAGTCTTATCATTCCTTCACCCCGCCCCCGTTCAGGGCTGGAGGTTGGCTTTGTCGAGGGTGGATCATTTGGAGCCGCCTGTAGTCGTCGTCTGACTGCCACCGAGCGTGGCTTGGACGTTGGCTTTGAGACCAACGCCGACATTCGAGAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTGAGGCGCGGTTATATCATCCACGCCCCGGTGGTTATTGCCATACCTGTACCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCGAGCACCGACCCCGCGAGGTTGAACCCGGTGGAGAAGCGGGTGAGCGTGGGGGAGAAGTGGGATCAGTTTGTGGTTTGCTGAACTAA", "aa": "MVFVALGVIPSSLIIPSPRPRSGLEVGFVEGGSFGAACSRRLTATERGLDVGFETNADIRERREKGTRTTCVRTSSAGRPRAGLRRGYIIHAPVVIAIPVPIPNPGNIIQHLVEHRPREVEPGGEAGERGGEVGSVCGLLN", "aa_hexdigest": "90cc5db1e0963df56dd341c84f152ab9", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0027E159D2", "ncbi_nrp_id": "WP_308010181.1", "uniref100_id": "UniRef100_UPI0027E159D2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0027E159D2", "RefSeq:WP_308010181.1", "UniRef:UniRef100_UPI0027E159D2" ] }, "ips": { "uniref100_id": "UniRef100_UPI0027E159D2", "uniref90_id": "UniRef90_UPI0027E159D2" }, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14928.934299999983, "isoelectric_point": 9.585820198059082 }, "id": "GIHJHMOALN_469", "locus": "GCA009939745_00434" }, { "type": "cds", "sequence": "contig_1", "start": 244265, "stop": 244432, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAGTCAAAACCAGGGTTCTACAACAACTACCTCAGCGGGCAATCCCGATTCTTTAGTTACTGATAAAGTTGATCAAAAAGGTCAGGTGCAAACAAGTGGTCAAAATTTAAGTGAGACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACATCCGACTGA", "aa": "MGSQNQGSTTTTSAGNPDSLVTDKVDQKGQVQTSGQNLSETNYTNLPPNLTPTSD", "aa_hexdigest": "93d85bf968dc2fb08c123730dd6d176c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5726.9822, "isoelectric_point": 4.1962182998657225 }, "id": "GIHJHMOALN_470", "locus": "GCA009939745_00435" }, { "type": "cds", "sequence": "contig_1", "start": 244514, "stop": 244774, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTTAATAAGAGTGGCGAAAATAACGAAAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGGCTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGA", "aa": "MLVNKSGENNEKFQATDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVETYFGTTRTSSTANQNSTTVPGIGLKFPNKIMIQRLC", "aa_hexdigest": "eb9d38ae99f201314af7b3da8d2accf4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9556.690999999993, "isoelectric_point": 8.818066215515138 }, "id": "GIHJHMOALN_471", "locus": "GCA009939745_00436" }, { "type": "cds", "sequence": "contig_1", "start": 245017, "stop": 245277, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD7934", "UniRef:UniRef90_UPI0034DD7934", "PFAM:PF03257.18" ], "nt": "GTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCTTTGGCCTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGACCAAACCCAATCCACTAACAGTTCCCCCTACCTGCACTTGGTGAAGCCTAAGAAAGTTACCAAATCCGACAAAGTTAGACGACGATCTTAA", "aa": "MESVWDLKGVWADQAQSDSQGSTTTATGATLPEHPNALAFQVSVVEASAYKPNTSSDQTQSTNSSPYLHLVKPKKVTKSDKVRRRS", "aa_hexdigest": "bb2c6b6b1154ee2210982f45b11f1a88", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034DD7934", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.988, "score": 163.0, "evalue": 4.44e-51, "valid": true, "uniref50_id": "UniRef50_UPI0034DD7934" }, "pscc": { "uniref50_id": "UniRef50_UPI0034DD7934", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034DD7934" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 38, "aa_cov": 0.43023255813953487, "hmm_cov": 1.0, "evalue": 2.4527035757122853e-15, "score": 52.50333786010742, "start": 46, "stop": 82 } ], "seq_stats": { "molecular_weight": 9335.184199999996, "isoelectric_point": 9.09972858428955 }, "id": "GIHJHMOALN_472", "locus": "GCA009939745_00437" }, { "type": "cds", "sequence": "contig_1", "start": 245312, "stop": 245506, "strand": "+", "frame": 2, "gene": null, "product": "Putative adhesin P1-like protein MPN_203", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50284", "UniRef:UniRef50_UPI0027E1A8D0", "UniRef:UniRef90_Q50284", "UniRef:UniRef90_UPI0027E1A8D0" ], "nt": "ATGCTCAAATCATTTGGTACAGAAAATTTCACCCAGCCCCAACCCCAACCCCAAGCCCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACCTCGGTAGTGTGCTTAGTGGTGGGGGGTACCACGCAGGGCTCAAGCACCACCAATCAACTGTCACCCGTTCAACGGGTGAGTGGGTGGACCGCTAA", "aa": "MLKSFGTENFTQPQPQPQALKTTTPVFGTSSGNLGSVLSGGGYHAGLKHHQSTVTRSTGEWVDR", "aa_hexdigest": "a8395a495eecbe98e740e258af41e552", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E1A8D0", "query_cov": 0.890625, "subject_cov": 1.0, "identity": 0.982, "score": 119.0, "evalue": 3.97e-34, "valid": true, "uniref50_id": "UniRef50_UPI0027E1A8D0" }, "pscc": { "uniref50_id": "UniRef50_UPI0027E1A8D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027E1A8D0" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 245123, "stop": 245506, "observations": { "mutation": [ 245141, 245228 ], "stop": [ 245141, 245228 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50284", "query_cov": 1.0, "subject_cov": 0.5039370078740157, "identity": 1.0, "score": 134.0, "evalue": 1.68e-39, "gene_start": 1, "gene_end": 64, "reference_start": 64, "reference_end": 127, "reference_sequence": "MLWPFRWVWWKRVLTSQTRAPAKPNPLTVPPTCTWWSLRKLPNPTKLDDDLKNLLDPNEVRARMLKSFGTENFTQPQPQPQALKTTTPVFGTSSGNLGSVLSGGGYHAGLKHHQSTVTRSTGEWVDR" }, "paralog": false, "identity": 0.9763779527559056, "score": 263.0, "evalue": 1.47e-92, "description": "Internal stop codon at 245141, 245228. Nonsense mutation around 245141, 245228.", "psc": { "uniref90_id": "UniRef90_Q50284", "product": "Putative adhesin P1-like protein MPN_203", "uniref50_id": "UniRef50_Q50284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50284", "UniRef:UniRef50_Q50284" ] }, "pscc": { "uniref50_id": "UniRef50_Q50284", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50284" ], "product": "Putative adhesin P1-like protein MPN_203" } }, "id": "GIHJHMOALN_473", "locus": "GCA009939745_00438" }, { "type": "cds", "sequence": "contig_1", "start": 245618, "stop": 245890, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574907.1", "SO:0001217", "UniParc:UPI0001DD64E7", "UniRef:UniRef100_A0AB38WAH1", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0AB38WAH1" ], "nt": "ATGAAATTGATTAAATTAGCGAGGTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTTCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTGTTGACACCTTTAAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAAAAGGAGGTATTACTTAATGAAAGAAAAAACTCCCTTTAA", "aa": "MKLIKLARFHRFLKQDQLQLVSLFSQNFLQVLLTPLNKPVNLFSENTTMKTNKKYAFAALLMQAQAKLPNSFKFFLSKKEVLLNERKNSL", "aa_hexdigest": "c706c9c9d50737294f2b4bb24c0c5725", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64E7", "ncbi_nrp_id": "WP_014574907.1", "uniref100_id": "UniRef100_A0AB38WAH1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64E7", "RefSeq:WP_014574907.1", "UniRef:UniRef100_A0AB38WAH1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38WAH1", "uniref90_id": "UniRef90_A0AB38WAH1" }, "psc": { "uniref90_id": "UniRef90_A0AB38WAH1", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10546.470099999991, "isoelectric_point": 10.505590629577636 }, "id": "GIHJHMOALN_474", "locus": "GCA009939745_00439" }, { "type": "cds", "sequence": "contig_1", "start": 246036, "stop": 246314, "strand": "+", "frame": 3, "gene": null, "product": "UPF0134 protein MPN_410", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572", "UniRef:UniRef90_P75572" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTAAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAGCAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGCTTGGACAAAATTGATCCATCTAAATAG", "aa": "MNTEYVTRKELNEYKDSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPSK", "aa_hexdigest": "05e342d677ef9c6d60d35fd88b1e7dc3", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 245868, "stop": 246314, "observations": { "mutation": [ 245949 ], "stop": [ 245949 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75572", "query_cov": 1.0, "subject_cov": 0.6216216216216216, "identity": 0.9890000000000001, "score": 111.0, "evalue": 1.31e-29, "gene_start": 1, "gene_end": 92, "reference_start": 57, "reference_end": 148, "reference_sequence": "MKEKTPFKNEKEFHEMVKKTKKGTFSGWYIIDKDNNSVEFSGKFNRQFKLNKPVITVNTEYVTRKELNEYKDSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPSK" }, "paralog": false, "identity": 0.9864864864864865, "score": 225.0, "evalue": 8.52e-77, "description": "Internal stop codon at 245949. Nonsense mutation around 245949.", "psc": { "uniref90_id": "UniRef90_P75572", "product": "UPF0134 protein MPN_410", "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75572", "UniRef:UniRef50_P75572" ] }, "pscc": { "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572" ], "product": "UPF0134 protein MPN_204" } }, "id": "GIHJHMOALN_475", "locus": "GCA009939745_00440" }, { "type": "cds", "sequence": "contig_1", "start": 246775, "stop": 247029, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGATAACACTGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCTGCGCGAACCGAGGCCACCTTGACCACCACCACCTTTGCGCTGCGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCAACGCGTGATGCCAACGGCCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGCAGTGCACCCCAACAGTGA", "aa": "MRDNTAKGITAGSGSQQTTYDPARTEATLTTTTFALRRYDLAGRALYDLDFSKLNPQTPTRDANGQITFNPFGGFGLSGSAPQQ", "aa_hexdigest": "1580632283a954a4f2070cb0b8e1ed18", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 57, "aa_cov": 0.6666666666666666, "hmm_cov": 0.8947368421052632, "evalue": 2.853755535080112e-08, "score": 28.89597511291504, "start": 28, "stop": 83 } ], "seq_stats": { "molecular_weight": 9019.777099999998, "isoelectric_point": 8.137860298156738 }, "id": "GIHJHMOALN_476", "locus": "GCA009939745_00441" }, { "type": "cds", "sequence": "contig_1", "start": 247060, "stop": 247560, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCCAAGACCCCACCGATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGTGTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGTTCGACCAATTCCGACACCAAAGTCAAGGCTTTAAAAATAGAGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGCTGCAGTTAGAAAAAAATGATCTCGCCAACGCTCCCATTAAGCGGGGTGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGA", "aa": "MAQDPTDPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGDESKSSNGSSSTSTTTQRGSTNSDTKVKALKIEVKKKSDSEDNGQLQLEKNDLANAPIKRGEESGQSVQLKADDFGTAPSSSGSGGNSNPGSPTP", "aa_hexdigest": "7a57e927cdc38ee00ed47581fc7b4353", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17354.60639999997, "isoelectric_point": 6.317733573913575 }, "id": "GIHJHMOALN_477", "locus": "GCA009939745_00442" }, { "type": "cds", "sequence": "contig_1", "start": 247522, "stop": 248046, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCCGACTTGGTAACATGGCCATTGCCACCAAACACCAAAAGCGCTTGCCCGAACCAGGTGAGGTTGCCAATGTTGGCGAAGTACGCTTCAAACGGGAGGGCGATGGGGGCCGACTTGTCGGAGTCAGCTTCCTTTTGAAAGCCCTGCTTGGTGTTCGAAGTATTACTTTCATCAAACCCGGTTTGGGTATTATCCGCGACCGCTTGGCCCTGGTCAAACCACTCGGGGTGGCGGCGCGGGTTGAAGAACCCGGTGGTTTGGAACAAAACATCGCGCGCCTTTCAGTCCCACAAACCGTGGTGGTTTCACTTGGGCGTTCTTCAACTGGGCGGATAGTTCGCGGTCATGGCCTTGGGATCCAAGTGATCAACGCGGTCAATGGCGGTACGGTTGCGCGCATAAGGCGCATCGTACAGAATCAGGATCGAGGCGGATCATTTGGGGAGGTCCTTGTGAATTTGCTCAGTCGCAAGCCACGGCCTTCAGGGGGTGGGGGAACCGGGGTTGGAGTTGCCGCCTGA", "aa": "MGRLGNMAIATKHQKRLPEPGEVANVGEVRFKREGDGGRLVGVSFLLKALLGVRSITFIKPGLGIIRDRLALVKPLGVAARVEEPGGLEQNIARLSVPQTVVVSLGRSSTGRIVRGHGLGIQVINAVNGGTVARIRRIVQNQDRGGSFGEVLVNLLSRKPRPSGGGGTGVGVAA", "aa_hexdigest": "135e833192f0291be6cf0b3ab844164a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18147.94999999998, "isoelectric_point": 11.777165031433107 }, "id": "GIHJHMOALN_478", "locus": "GCA009939745_00443" }, { "type": "cds", "sequence": "contig_1", "start": 248740, "stop": 248964, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCCGATTGCCGTTTTACCTATTGCGGGTATTTTTTTGGGTGTTGGTGATGCAATTGCCGGAAATGCAGGTGATTTAACTGGTCTGCGTTATTTCGGACTTTTCATTAAAAATGGTGGTGATGTTGTTTTTGCTAACCTACCCATTTTGTTTGCTATTGCTATAGCAATAACGTTCTCACAAGACGCTGGGGTCGCCGGATTTTCGGCCTTTGTCTTTTGA", "aa": "MIPIAVLPIAGIFLGVGDAIAGNAGDLTGLRYFGLFIKNGGDVVFANLPILFAIAIAITFSQDAGVAGFSAFVF", "aa_hexdigest": "14e5d9a47c587e87911697fbcf380fdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7518.7656, "isoelectric_point": 4.138697242736816 }, "id": "GIHJHMOALN_479", "locus": "GCA009939745_00444" }, { "type": "cds", "sequence": "contig_1", "start": 248971, "stop": 249297, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACGGGTTTATGAGCTCGTTAATATTGCCTTTTGATAAAGCAGGCAAAATTATTACTGACACTTCGACTCCAATTGCTGGTTTTAAAGTACTTTACTACAAGAGTGTCCCTGTTCATGCTATTGCTACAACCTTGGGACTGCGAACATTATCAACCTCAGTATTCGGTGGGATTATTGTAGGTGCCTTAACTTCAGTACTTTACAAGAAGTTTTATGCCATTAGGTTACCTGATGTAATTGGATTCTTTAGCGGTACTCGTTTTGTCCCTATTATTTGTTTTGTGGTAGCGATTCCTGTTGCGTTAATCTTGTTAATGATCTGA", "aa": "MNGFMSSLILPFDKAGKIITDTSTPIAGFKVLYYKSVPVHAIATTLGLRTLSTSVFGGIIVGALTSVLYKKFYAIRLPDVIGFFSGTRFVPIICFVVAIPVALILLMI", "aa_hexdigest": "ae6474d1b9b520b7a4a9996aa1460cab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11629.966399999988, "isoelectric_point": 9.843049049377441 }, "id": "GIHJHMOALN_480", "locus": "GCA009939745_00445" }, { "type": "cds", "sequence": "contig_1", "start": 249787, "stop": 250245, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTCTGAACGGTTCTGGTATTTTAGGTAAAAAATTGTTACTAAGTGATGGTAATGTTTACACTATTACCTTTAAAACCTTTGCCAACACTACTCCAATTGCTTGGAGTAAAGGTGCACAGGCAGTGTTACCTTTAAATGCTAGCTCTACAATCGTTAACAATCCAACCGCTTTAGCAGCAGCTACACAAAGTAATAATAACACTAACAACATTAAACTTTACCCGGTAAATTCCTTTAGGGTAGCTGTTGAAAGTTTAAATCCAGCGCAATACTCCCAGGGTAAATTCCCCTTCATGTTGTTTGGAATTCCAGCAGCTGGTGTAGCCATGATCTTGGCAGCACCTAAAGATAGAAGAAAGGAAGCAGCTTCGATTGTTGGTAGTGCAGCATTTACCAGCTTCTTAACTGGTATTACTGAACCATTTGAATTTACCTTCTTGTTCTTAGCGCCTTGA", "aa": "MGLNGSGILGKKLLLSDGNVYTITFKTFANTTPIAWSKGAQAVLPLNASSTIVNNPTALAAATQSNNNTNNIKLYPVNSFRVAVESLNPAQYSQGKFPFMLFGIPAAGVAMILAAPKDRRKEAASIVGSAAFTSFLTGITEPFEFTFLFLAP", "aa_hexdigest": "e731937ab6c91344ce87cd581481d22b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16049.296199999986, "isoelectric_point": 9.75440502166748 }, "id": "GIHJHMOALN_481", "locus": "GCA009939745_00446" }, { "type": "cds", "sequence": "contig_1", "start": 250294, "stop": 251433, "strand": "+", "frame": 1, "gene": "ptsG", "product": "PTS system glucose-specific EIICBA component", "db_xrefs": [ "COG:COG2190", "COG:G", "EC:2.7.1.199", "GO:0005886", "GO:0008982", "GO:0009401", "GO:0016301", "GO:0090563", "SO:0001217", "UniRef:UniRef50_P47315", "UniRef:UniRef90_P75569" ], "nt": "ATGAACATCTTGGGTGCTAATGTGGGTCAAACCTTCTCTGGTTCCTTTATTGACTTTATTCTCTATGGTGCTTTACCAGATGGTCGTCGTTGGTTAGCAAACTCTTACTTAGTACCTATTATTGGACTGTTCTTAGCTGCCATCTACTTCCCAACCTTCTACTTCTTAATTAAGCACTTTAACTTGGCAACTCCAGGTCGTGGTGGTAAGTTAATTACCAAGAAGGAATACTTAGCAAGCAAAGCAGCCGCTAAAGCAGAGGGTGTAAGTGGTGTAGCAGAAAACTTTACCCAAACGCAAATTGAAGCGGGCATCTTACTCCAAGCTTACGGTGGCAAGGAAAACATTGTTGAACTAGGTGCATGTATTACCAAGTTACGGGTTACTGTTAAGAACCCCGAACTAGTTAAGGAAGAACCTATTAAAGAACTTGGAGCTGCTGGGGTAATGCGTACCACCCCAACCTTCTTTGTAGCCGTTTTTGGTACCCGCGCTGCCGTTTACAAATCTGCTATGCAGGACATTATCCAGGGCAAGGTTAACTGGGAAGCGTTGCAAAAGGTTATTAATACAGATCAACTAGCTGTTGAGCCTAAAGAAACCACTCCACCAAAGGAAGTAATGCCGGTGGTACAAGACGAAATTGTGATTCTGTCCCCAGTTAACGGTACCTTAAAATCACTCAATCAAGTACCCGATGAAACCTTTAAGCAAAAGCTAGTGGGTGAAGGTGTTGCCATTGTACCGAGCGATGGTCACTTTAAAGCCCCTGGTGAAGCAGGTGTGAAAACGGAGTTAGCTTTCCCTGGTGGTCACGCTTACATCTTTGACATTGATGGCATTAAGGTAATGTTGCACATTGGGATTGACACTGTGCAAATTAACGCCAAAAAACAACCGGGCGAACCACTAGAAGTGTTTGACATTAAGACCAAACAGGGTGAGTACACTAAGGAAAAGAGTGAAAGTGTAGTGGAAGTTGATTTAAAGAAACTCAGCAAGAAGTACAACCCAATTACCCCATTTGTGGTAATGAAGGAATCACTAGAAAACTTTAAATTAGTGCCAATCCGCCAACGCGGTGAGATTAAAGTGGGCCAACCAATCTTTAAACTAGTCTACAAGAAGTCACAGGCATAA", "aa": "MNILGANVGQTFSGSFIDFILYGALPDGRRWLANSYLVPIIGLFLAAIYFPTFYFLIKHFNLATPGRGGKLITKKEYLASKAAAKAEGVSGVAENFTQTQIEAGILLQAYGGKENIVELGACITKLRVTVKNPELVKEEPIKELGAAGVMRTTPTFFVAVFGTRAAVYKSAMQDIIQGKVNWEALQKVINTDQLAVEPKETTPPKEVMPVVQDEIVILSPVNGTLKSLNQVPDETFKQKLVGEGVAIVPSDGHFKAPGEAGVKTELAFPGGHAYIFDIDGIKVMLHIGIDTVQINAKKQPGEPLEVFDIKTKQGEYTKEKSESVVEVDLKKLSKKYNPITPFVVMKESLENFKLVPIRQRGEIKVGQPIFKLVYKKSQA", "aa_hexdigest": "a5a856f4cedb2962e2a773fd71348971", "start_type": "ATG", "rbs_motif": null, "genes": [ "ptsG" ], "truncated": "5-prime", "pseudogene": { "start": 249994, "stop": 251433, "observations": { "mutation": [ 250243 ], "stop": [ 250243 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75569", "query_cov": 1.0, "subject_cov": 0.4031914893617021, "identity": 1.0, "score": 731.0, "evalue": 1.55e-257, "gene_start": 1, "gene_end": 379, "reference_start": 562, "reference_end": 940, "reference_sequence": "MQIKAQDTGQQKKSCLLSNIRNKWKNRNRGSFRQWVGKLSNGLMIPIAVLPIAGIFLGVGDAIAGNAGDLTGLRYFGLFIKNGGDVVFANLPILFAIAIAITFSQDAGVAGFSAFVFWAAMNGFMSSLILPFDKAGKIITDTSTPIAGFKVLYNKSVPVHAIATTLGLRTLSTSVFGGIIVGALTSVLYKKFYAIRLPDVIGFFSGTRFVPIICFVVAIPVALILLMIWPAVSIGLNAIGTGLGFLGGKGYGANSLIFGYIERSLIPFGVHHAFYAPLWYTSAGGSLQEIVNQQVWIRPDFHLSDNYVARVIGWVDPNNSSMYIIPGALNGQNGSSTGNTMSKDLNGALSAYMSKESTAFLTWKDLVDGLTFKGNFDKMAENGLLDGSNKIWLGLNGSGILGKKLLLSDGNVYTITFKTFANTTPIAWSKGAQAVLPLNASSTIVNNPTALAAATQSNNNTNNIKLYPVNSFRVAVESLNPAQYSQGKFPFMLFGIPAAGVAMILAAPKDRRKEAASIVGSAAFTSFLTGITEPFEFTFLFLAPWLFYGVHAVLAAVSFWLMNILGANVGQTFSGSFIDFILYGALPDGRRWLANSYLVPIIGLFLAAIYFPTFYFLIKHFNLATPGRGGKLITKKEYLASKAAAKAEGVSGVAENFTQTQIEAGILLQAYGGKENIVELGACITKLRVTVKNPELVKEEPIKELGAAGVMRTTPTFFVAVFGTRAAVYKSAMQDIIQGKVNWEALQKVINTDQLAVEPKETTPPKEVMPVVQDEIVILSPVNGTLKSLNQVPDETFKQKLVGEGVAIVPSDGHFKAPGEAGVKTELAFPGGHAYIFDIDGIKVMLHIGIDTVQINAKKQPGEPLEVFDIKTKQGEYTKEKSESVVEVDLKKLSKKYNPITPFVVMKESLENFKLVPIRQRGEIKVGQPIFKLVYKKSQA" }, "paralog": false, "identity": 0.9979123173277662, "score": 938.0, "evalue": 0.0, "description": "Internal stop codon at 250243. Nonsense mutation around 250243.", "psc": { "uniref90_id": "UniRef90_P75569", "gene": "ptsG", "product": "PTS system glucose-specific EIICBA component", "ec_ids": [ "2.7.1.199" ], "uniref50_id": "UniRef50_P47315", "cog_id": "COG2190", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0016301", "GO:0008982", "GO:0090563", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75569", "EC:2.7.1.199", "UniRef:UniRef50_P47315", "COG:COG2190", "COG:G", "GO:0005886", "GO:0016301", "GO:0008982", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47315", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47315" ], "product": "PTS system glucose-specific EIICBA component" } }, "id": "GIHJHMOALN_482", "locus": "GCA009939745_00447" }, { "type": "cds", "sequence": "contig_1", "start": 252043, "stop": 252378, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAACTGATTACAACACCTGTAGAAACCACCGCCAAAGCGGAACTCGTTTCCCTAGCTAAGCTCGGGGAAATGCGTACCCATGTCGGAATGGTTAAGCGTTACTGGAACCCCAAAATGGGCTTTTTTATTGAACCAGAACGCAAGCACAATAACGATCACTTTGTATTAGAGTTACAACGTCAATCTTTGCAAACAGCTTACAACTACGTCAAGGAAGTAGCACAAAACAACGGTCAAATTCTCTTCGTTGGTACTAAGAACGATTATGTGAAAAAACTCGTTAATAATATCGCTAAACGGGTTCATGTTGCTTTTATTACTCAGCGCTGA", "aa": "MSELITTPVETTAKAELVSLAKLGEMRTHVGMVKRYWNPKMGFFIEPERKHNNDHFVLELQRQSLQTAYNYVKEVAQNNGQILFVGTKNDYVKKLVNNIAKRVHVAFITQR", "aa_hexdigest": "ede2eb867c81c97584ea6d007c805a42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12861.739699999984, "isoelectric_point": 9.740608787536619 }, "id": "GIHJHMOALN_483", "locus": "GCA009939745_00448" }, { "type": "cds", "sequence": "contig_1", "start": 252379, "stop": 252927, "strand": "+", "frame": 1, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02967", "SO:0001217", "UniRef:UniRef50_UPI00132F76CA", "UniRef:UniRef90_UPI00132F76CA" ], "nt": "TTGGGTGGTACTTTAACTAACTTTAAAACACTTTCAATTTCAATTAATAAACTCAATAAGTTAGTGGAAAAACAAGCGGAAAACGCGGCTGATTTAACTAAAAAGGAAAACTTGATGCTTTCCCGCGAAATTGAACGCTTGGAGAAGTTCTTTGGCGGGGTAAAATCACTTAAACGTTTACCGAACTTACTAATTGTGGATGACCCGGTATACGAAAAGAATGCGGTAGCGGAGGCTAACAGCTTGAGAATTCCTGTAGTAGCGTTGTGTAACACTAACACCAATCCGGAATTAGTTGACTTTATTATTCCCGCTAACAACCACCAACCCCAATCAACCTGCTTGTTGATGAACTTATTAGCTGATGCGGTGGCTGAAGCTAAGGCAATGCCAACCATGTTTGCTTACAAACCGGATGAGGAAATTCAAATTGAAATTCCCCAAAAACAAGAGGCACCACGCCAAGTTGTAAACCGTGCAAATTCTAAACAAATTACCTCTCAACGGTTAAACATTACTCGCAACCCAGAAGTTCTCACCCGTGAATAA", "aa": "MGGTLTNFKTLSISINKLNKLVEKQAENAADLTKKENLMLSREIERLEKFFGGVKSLKRLPNLLIVDDPVYEKNAVAEANSLRIPVVALCNTNTNPELVDFIIPANNHQPQSTCLLMNLLADAVAEAKAMPTMFAYKPDEEIQIEIPQKQEAPRQVVNRANSKQITSQRLNITRNPEVLTRE", "aa_hexdigest": "2d2a9480b5bba35f7e805dbaaced282e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00132F76CA", "query_cov": 1.0, "subject_cov": 0.9054726368159204, "identity": 0.995, "score": 344.0, "evalue": 3.18e-119, "valid": true, "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_UPI00132F76CA", "kegg_orthology_id": "K02967", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00132F76CA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132F76CA" ], "product": "30S ribosomal protein S2" }, "genes": [ "rpsB" ], "id": "GIHJHMOALN_484", "locus": "GCA009939745_00449" }, { "type": "cds", "sequence": "contig_1", "start": 253181, "stop": 253735, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGCCAACTCCCTGATTGGCTCCATTCAGGAATTTAAAGCGCAAAAGTCAGCTCACACCTTAAAGTCCCTAACGCAACCCTTTACGCGGGTATTCCGTGAAGAAGGCTTGGTTTCGTTACCGGTGGGTGAAGTTGTTGTCGGTGACATTATCTTTCTGGAAGCGGGTGATATTATTCCCGCTGACGGGAAGGTGCTGCAAGCGAACCACCTCCGCTGTATGGAGAGCTTTTTAACTGGTGAATCAGTCCCAGTTGATAAAAGTGTGGTTAACACCGGTGGCAAGGGTTTGTTGGAACAAACAAACCTACTCTTTTCTGGTGCCCAAGTGGTCTTTGGTAGTGGTGTATTCGAAGTAACTGCTGTGGGGTTAAATACCCAAGTCGGTCAAATTGTCAAAACAGTGGACAGTAGTGCAACTAAGCTATCGCCCCTACAGCAAAAACTGGAAAAAGTGGGGAAGTGGTTTAGCTGGTTTGGCCTTGGTTTATTCGTTGTCGTTTTCCTAGTGCAGTTAGGGTTACTTGGTTTTCACAACTTTAGCGCTAACTGA", "aa": "MLANSLIGSIQEFKAQKSAHTLKSLTQPFTRVFREEGLVSLPVGEVVVGDIIFLEAGDIIPADGKVLQANHLRCMESFLTGESVPVDKSVVNTGGKGLLEQTNLLFSGAQVVFGSGVFEVTAVGLNTQVGQIVKTVDSSATKLSPLQQKLEKVGKWFSWFGLGLFVVVFLVQLGLLGFHNFSAN", "aa_hexdigest": "cde4ae56015048cc887e0fd91da2a783", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19722.599000000002, "isoelectric_point": 6.286415290832521 }, "id": "GIHJHMOALN_485", "locus": "GCA009939745_00450" }, { "type": "cds", "sequence": "contig_1", "start": 253793, "stop": 254644, "strand": "+", "frame": 2, "gene": null, "product": "cation-transporting P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A405064", "UniRef:UniRef90_UPI000A405064" ], "nt": "GTGACCTTTATTAACGTCATTTTTGCCTTAAGTGTGCAGAAGCTCACCAAACAAAAGGCAATTATTAAGTACCTAGCTGCAATCGAAACACTCGGTGGCGTGCAAATCATTTGTACCGACAAAACTGGTACTTTAACACAAAACAAGATGAAGGTCGTGGACTATTTCTGTTTTAGTAACACTACCCAAACAGACTTAGCACGTGCTTTGTGTTTGTGCAACAATGCTACAGTCAACACTAACGGAGACAGCACCGGTGATCCAACTGAAATTGCCCTTTTGCAATGGTTGGACCGTGATGGTTTGGAATTAAACCACTACACACGCGTGTACGAAAAGGCATTTGATTCCAACCGCAAGCTTATGAGTGTGGTGGTGCAAAAGGACAACCGTTTTATTGTGATTGTCAAGGGCGCACACGATGTCTTGTTACCGTTATGTAAGGGCTTGGATAGCAACCAAATTAAGCCTTTAATTGATGAACGTGCTAGCAACGGCTTGCGCAACTTAGCGGTGGGACTCAAGGTTTTATACTGCTTTGATCCAGAAAATACCCAAACTGTCAACGAATTGGAAAGCGAACTCGACTTTTTGGGTTTTGTTAGCTTACAAGATCCACCACGAATTGAAAGTAAAGCTGCCATTATGGCGTGTCACCAAGCCAACATTACCCCAATTATGATCACTGGTGATCACCTCAAAACCGCTACTGCAATTGCCAAAGAATTGGGCATTCTGACCGATGAAAGGCAAGCCATTTTAGGAGTCGATTTAGACCCCGCTAAAATTATGGAGTACCGCGTCTTTGCCAGAGTAACGCCGCAGCAAAAGCTAGAAATAGTTAATGCCTGA", "aa": "MTFINVIFALSVQKLTKQKAIIKYLAAIETLGGVQIICTDKTGTLTQNKMKVVDYFCFSNTTQTDLARALCLCNNATVNTNGDSTGDPTEIALLQWLDRDGLELNHYTRVYEKAFDSNRKLMSVVVQKDNRFIVIVKGAHDVLLPLCKGLDSNQIKPLIDERASNGLRNLAVGLKVLYCFDPENTQTVNELESELDFLGFVSLQDPPRIESKAAIMACHQANITPIMITGDHLKTATAIAKELGILTDERQAILGVDLDPAKIMEYRVFARVTPQQKLEIVNA", "aa_hexdigest": "e5039c6d0d8a6fe02b4597dc799f2781", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 253493, "stop": 254555, "observations": { "insertion": [ 254480 ], "mutation": [ 253733 ], "stop": [ 253733, 254529 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A405064", "query_cov": 0.8056537102473498, "subject_cov": 0.41834862385321103, "identity": 1.0, "score": 450.0, "evalue": 2.47e-154, "gene_start": 2, "gene_end": 229, "reference_start": 293, "reference_end": 520, "reference_sequence": "MNKWTGLSAAAVLESRAQHGANLIPTKKLTPFWLLFLEQFKSLVVILLLVATILSLVVAIISGVNANWLFDHNLVIEWTQPFVILITVLANSLIGSIQEFKAQKSAHTLKSLTQPFTRVFREEGLVSLPVGEVVVGDIIFLEAGDIIPADGKVLQANHLRCMESFLTGESVPVDKSVVNTGGKGLLEQTNLLFSGAQVVFGSGVFEVTAVGLNTQVGQIVKTVDSSATKLSPLQQKLEKVGKWFSWFGLGLFVVVFLVQLGLLGFHNFSANWSIALIGAIALVVAIIPEGLVTFINVIFALSVQKLTKQKAIIKYLAAIETLGGVQIICTDKTGTLTQNKMKVVDYFCFSNTTQTDLARALCLCNNATVNTNGDSTGDPTEIALLQWLDRDGLELNHYTRVYEKAFDSNRKLMSVVVQKDNRFIVIVKGAHDVLLPLCKGLDSNQIKPLIDERASNGLRNLAVGLKVLYCFDPENTQTVNELESELDFLGFVSLQDPPRIESKAAIMACHQANITPIMITXVITSKPLLQLPKNWAFWPMKGKPF" }, "paralog": false, "identity": 0.9915254237288136, "score": 680.0, "evalue": 5.17e-247, "description": "Internal stop codon at 253733, 254529. Frameshift due to insertion around 254480. Nonsense mutation around 253733.", "psc": { "uniref90_id": "UniRef90_UPI000A405064", "product": "cation-transporting P-type ATPase", "uniref50_id": "UniRef50_UPI000A405064", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A405064", "UniRef:UniRef50_UPI000A405064" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A405064", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A405064" ], "product": "cation-transporting P-type ATPase" } }, "id": "GIHJHMOALN_486", "locus": "GCA009939745_00451" }, { "type": "cds", "sequence": "contig_1", "start": 254729, "stop": 255124, "strand": "+", "frame": 2, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA", "UniRef:UniRef90_UPI001FB434DA" ], "nt": "ATGGGACAAACGGGGGTTGACATTGCCAAAGATGCCGCTGATGTCATTATTAGCGATGACAACTTTGCCACAATTGTTAACGGGATTGAACAGGGTCGTAAAACCTTTTTGACCTGTAAACGGGTGTTGTTTAACCTCTTTTTAACCTCAATTGCTGGCACGATTGTCGTGTTATTGGGTTTATTTGTATTAGGGGAAGTCTTTCGTGAACAACTCAGCAAAGCAAACCATAACTTCCAGGTCTTTACCCCTACGCAACTGCTAATTATTAACCTCTTTGTTCACGGCTTTCCCGCTGTGGCCTTAGCGATCCAACCCGTCCAGGAAAAACTGATGTTAAAACCGTTTTCCACCAAAAATCTGTTTTACAACCGTGGCGGGTTCGATTTAATCTGA", "aa": "MGQTGVDIAKDAADVIISDDNFATIVNGIEQGRKTFLTCKRVLFNLFLTSIAGTIVVLLGLFVLGEVFREQLSKANHNFQVFTPTQLLIINLFVHGFPAVALAIQPVQEKLMLKPFSTKNLFYNRGGFDLI", "aa_hexdigest": "dbc40f381a642a7fb508399d7de6d4db", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 254519, "stop": 255346, "observations": { "mutation": [ 254642, 255122 ], "stop": [ 254642, 255122 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FB434DA", "query_cov": 1.0, "subject_cov": 0.4729241877256318, "identity": 1.0, "score": 255.0, "evalue": 7.69e-84, "gene_start": 1, "gene_end": 131, "reference_start": 71, "reference_end": 201, "reference_sequence": "MGILTDERQAILGVDLDPAKIMEYRVFARVTPQQKLEIVNAWKQAGYTVAVTGDGVNDAPALVTSDVGCCMGQTGVDIAKDAADVIISDDNFATIVNGIEQGRKTFLTCKRVLFNLFLTSIAGTIVVLLGLFVLGEVFREQLSKANHNFQVFTPTQLLIINLFVHGFPAVALAIQPVQEKLMLKPFSTKNLFYNRGGFDLIWQSLLLSFLTLLFYSLGMVYAINDPELGKSGDLINRAGATCGFMVLGGSAALNSLNLMVDRPLVATNPKHYGIVXX" }, "paralog": false, "identity": 0.9890909090909091, "score": 531.0, "evalue": 4.29e-195, "description": "Internal stop codon at 254642, 255122. Nonsense mutation around 254642, 255122.", "psc": { "uniref90_id": "UniRef90_UPI001FB434DA", "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FB434DA", "UniRef:UniRef50_UPI001FB434DA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA" ], "product": "HAD-IC family P-type ATPase" } }, "id": "GIHJHMOALN_487", "locus": "GCA009939745_00452" }, { "type": "cds", "sequence": "contig_1", "start": 255137, "stop": 255346, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCTTTTTAACCCTACTTTTCTACAGTTTAGGGATGGTCTATGCCATTAATGATCCGGAGTTAGGCAAATCCGGTGATTTAATTAACCGGGCTGGTGCGACCTGTGGTTTTATGGTGTTAGGGGGCAGTGCAGCTTTAAATTCCTTAAACCTAATGGTGGATCGACCATTGGTAGCTACTAACCCAAAGCACTATGGCATTGTGTGA", "aa": "MSFLTLLFYSLGMVYAINDPELGKSGDLINRAGATCGFMVLGGSAALNSLNLMVDRPLVATNPKHYGIV", "aa_hexdigest": "90d89e9adaf1a3081a1b760d3f328d08", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7320.5107000000025, "isoelectric_point": 6.508485221862792 }, "id": "GIHJHMOALN_488", "locus": "GCA009939745_00453" }, { "type": "cds", "sequence": "contig_1", "start": 255356, "stop": 255538, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCCTCGATCTTTGTCTTCTTATTAATTATTTTCATTAACCCCTTGGGGTTAGTGTTTAGTACATTAAAAGACCTTACTGCCCACCCGGTTTTAATTGGTTATAGCTTTGGGGGCGTGCTTTTATATATGACAATTAACGAAGTTGTTAAACTTATTAGGTTAAGTTATGGGTCTGTTTAA", "aa": "MSSIFVFLLIIFINPLGLVFSTLKDLTAHPVLIGYSFGGVLLYMTINEVVKLIRLSYGSV", "aa_hexdigest": "38c61e2afc5f1f446e6fe3015195232c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6634.966199999999, "isoelectric_point": 8.160553169250488 }, "id": "GIHJHMOALN_489", "locus": "GCA009939745_00454" }, { "type": "cds", "sequence": "contig_1", "start": 255525, "stop": 257687, "strand": "+", "frame": 3, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002", "KEGG:K03070", "SO:0001217", "UniRef:UniRef50_P47318", "UniRef:UniRef90_P75559" ], "nt": "ATGGGTCTGTTTAACTTCTTGAAGTTAGTCTCCCCGCGTCATCGGATTTACCATAAGGCAAGTAAGATTGCCAACGAAGTCGAGGGACACAAAAATTACTACCGCAACCTCACTGATGTACAGCTGCTGGAAGAGTCCAATAAGCTAGTGGATTTAGTGACAAAGCAAAACTACACCATTTTGGATGTGGCGGTGGCTGCTTTGGCCTTAATTCGCGAAGTGGTCTACCGTGAAACCGGGGAGTTTGCTTACAGAGTGCAAATTATTGGTGCTTACATCGTCTTGATCGGTGACTTTGCTGAAATGATGACTGGGGAAGGGAAAACCCTGACCATTGTTTTAGCAGCCTATGTCTCGGCGTTAGAAAAACGCGGTGTCCATGTGGTTACGGTCAATGAATACCTAGCGCAGCGGGATGCTACTAACGCCACCAAGATCTTAAAGCGTGTTGGCATGACCGTTGGTTGTAACTTTGCTAATCTAGCACCACATTTAAAGCAAGCCGCGTTTGCTTGTGATGTAACTTACACCACTAACAGTGAACTCGGTTTTGATTATCTCAGGGACAATATGGTGCACCGCTTTGAGGACAAGAAGATCCGCGAGTTGCACTTTGCGATAGTAGACGAAGGCGATTCGGTTCTCATTGATGAAGCGCGCACCCCTTTAATTATTTCTGGTCCTGCCAAAAACGAGTTTGCAGCATACGTGGCTGTTGATCGCTTTGTCAAAAAGCTCAAGGAAGACGAGTACAAGATTGACCCCGAATCACGTGCCCCCGCTTTAACAGAACTTGGCATTAAGCACGCGGAAAAGAACTTTAAAACCGATAACCTGTTTGCGCTAGAAAACAGTGACCTGTTCCACAAGATTATTAACGCTTTAACCGCCGTTAAAGTTTTTGAGCAGGGCAAGGAGTACATTGTGCGTGATGGTAAGGTGTTAATTGTGGACCACTTTACTGGCCGAATCCTGGAGGGCAGGAGCTACAGTAATGGGCTCCACCAAGCGGTTCAGGCCAAGGAAATGGTCGAAATTGAACCAGAAAATGTGATAGTAGCTACGATTACCTACCAATCGTTTTTCCGTCTGTACAACCGGTTGTCAGCGGTATCGGGTACCGCTTTTACTGAATCGGAGGAATTCCTCAAGATTTACAACATGGTGGTAGTACCCGTACCTACTAACCGACCCAATATCCGCAAAGACCGTGCTGACAGCGTCTTTGGTACGCCCAACATTAAGTGGCTAGCAGTGGTCAAGGAAGTCAAACGGATCCACGAAACCGGGCGACCAATCCTAATTGGGACCGCCAACATTGATGACTCGGAACTACTTCACAACTACCTCCAAGAAGCCAACATTCCCCACGAAGTTCTCAACGCCAAGAACCACTCTCGGGAAGCGGAAATAGTCGCTAAGGCGGGCCAAAAAGGGGCGGTGACCATTTCTACCAATATGGCGGGAAGAGGTACTGACATTCGTTTAGGTGAAGGGGTCGCTGAAATGGGCGGGTTGTATGTCCTTGGTACGGAACGCAACGAATCGCGCCGGATTGACAACCAACTGCGCGGTCGGGCGGGTCGGCAAGGCGATCGCGGTGAGACCAAGTTCTTTATCTCCTTGGGTGATGCCCTGTTCAAACGCTTTGCCCATGACCGGATTGAACGCGCCATTACTAAGTTAGGGAACGATACCTTTGACAGCTCCTTCTTTTCCAAGATGTTAAGCCGTACCCAAAAACGGGTCGAAGCAATTAACTTTGACACCAGAAAGAACTTAATTGATTATGACCATGTGTTGGCCAGTCAACGGGAATTGATCTACAAACAACGCGATAAGTTCTTATTAGCCACTGATCTCAGTGACATGATCGACAAGATGTTGGAAAAGTTTGTCGAGCAGTTCTGTGACCAGTACCGCAACCCTAAGAACCAAAACCTCGTTAACCACATTGCTTTATCAGAGGCCTTAAACCTGGAATTAAACATGCACGGTGTTATTAGTCCGAAGCTGTTTGAAAACATGACTTTTGATGCGACTGTCCATAAAACCCACAGTTTAATTGGTGAAAAGATTACCAATAAGGTCAAGGTCTTAACCCCCCCAATTGCCCTCATCCGTTTTCGTGAAATTATGATCACAGCAATGGACAAGCACTGA", "aa": "MGLFNFLKLVSPRHRIYHKASKIANEVEGHKNYYRNLTDVQLLEESNKLVDLVTKQNYTILDVAVAALALIREVVYRETGEFAYRVQIIGAYIVLIGDFAEMMTGEGKTLTIVLAAYVSALEKRGVHVVTVNEYLAQRDATNATKILKRVGMTVGCNFANLAPHLKQAAFACDVTYTTNSELGFDYLRDNMVHRFEDKKIRELHFAIVDEGDSVLIDEARTPLIISGPAKNEFAAYVAVDRFVKKLKEDEYKIDPESRAPALTELGIKHAEKNFKTDNLFALENSDLFHKIINALTAVKVFEQGKEYIVRDGKVLIVDHFTGRILEGRSYSNGLHQAVQAKEMVEIEPENVIVATITYQSFFRLYNRLSAVSGTAFTESEEFLKIYNMVVVPVPTNRPNIRKDRADSVFGTPNIKWLAVVKEVKRIHETGRPILIGTANIDDSELLHNYLQEANIPHEVLNAKNHSREAEIVAKAGQKGAVTISTNMAGRGTDIRLGEGVAEMGGLYVLGTERNESRRIDNQLRGRAGRQGDRGETKFFISLGDALFKRFAHDRIERAITKLGNDTFDSSFFSKMLSRTQKRVEAINFDTRKNLIDYDHVLASQRELIYKQRDKFLLATDLSDMIDKMLEKFVEQFCDQYRNPKNQNLVNHIALSEALNLELNMHGVISPKLFENMTFDATVHKTHSLIGEKITNKVKVLTPPIALIRFREIMITAMDKH", "aa_hexdigest": "1ac3bdcbdafd767446dd7a8ddd0a26a8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75559", "query_cov": 1.0, "subject_cov": 0.8910891089108911, "identity": 1.0, "score": 1370.0, "evalue": 0.0, "valid": true, "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_P47318", "cog_id": "COG0653", "cog_category": "U", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0005829", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ] }, "pscc": { "uniref50_id": "UniRef50_P47318", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47318" ], "product": "Protein translocase subunit SecA" }, "genes": [ "secA" ], "id": "GIHJHMOALN_490", "locus": "GCA009939745_00455" }, { "type": "cds", "sequence": "contig_1", "start": 257742, "stop": 257951, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAACGAGTCCCCTCAACGTTTACATTCGCGAAACCGACATCTTGTTCCAAACAATGTTGCAAAAGATTGCTCGGGACGTCATTATCCAAATTGCTAACTTAGCGACTCCCGAAGAGTTTGATGAGGAACTAATGAAGGCTAACGCCTTGAAGAAGTTGCAAGCACTTAGAGAAGCTCATGAAAAGTCCAACCAAGGACAGTAA", "aa": "MEQTSPLNVYIRETDILFQTMLQKIARDVIIQIANLATPEEFDEELMKANALKKLQALREAHEKSNQGQ", "aa_hexdigest": "1117da0cfbdeb69604f76a8b26d378b9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7957.036599999999, "isoelectric_point": 4.997818565368651 }, "id": "GIHJHMOALN_491", "locus": "GCA009939745_00456" }, { "type": "cds", "sequence": "contig_1", "start": 257926, "stop": 259899, "strand": "+", "frame": 1, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887", "KEGG:K03702", "RefSeq:WP_010874568.1", "SO:0001217", "UniParc:UPI0000137EBA", "UniRef:UniRef100_P75558", "UniRef:UniRef50_P47319", "UniRef:UniRef90_P75558" ], "nt": "ATGAAAAGTCCAACCAAGGACAGTAAACTGTTCCACCTCAAGAGTAACTTTGCCCCCACTGGGGACCAACCAGCGGCGATTGCTAAGTTAGCTGAGTTTCAAACCAATGAACAGGTCTTACTCGGTGCTACGGGTACCGGTAAGACCTTTACGATTGCCAACGTTATTCAAAAGGTACAACTGCCTACAGTAGTGATTGCCCACAATAAAACGTTAGCGGGACAGCTTTATCAGGAGTTGAAGGAGTTATTCCCTAATAATGCGGTGGAGTACTTCATCTCCTACTTTGACTTTTACCAACCAGAGGCTTATCTGCCCGCTAAGGGGGTTTACATTGAAAAGAGTGCAACTGTTAACGAAGAGATTAAACGGTTACGAGTGTCCACCTTACATTCACTCTCTACCCGCAAGGATGTGATTGTGGTGGGTTCGGTAGCCAGTATTTACCCAACTTCTTCTCCCGCTGATTTCGCCCAGTACTCGCTCTGGTTAGTAGTTGGTAAGGAATACGGTTTAAGTGAGTTAAAAACGCAGTTAATTCACCTAAACTATGTTGTTAATAAACAACAGTTAACTCCGGGAAAGTTCCGCTTTCAGGGCGATGTTGTTGAAGTGTTTCCTGGTTATGCGCAGGATTATGTCTTGCGGCTTTCCTTCTTTGACCAGCAACTAGAACAAATTGCTCGCATCGATCCCTTAACTAACAAGGTATTGGAAACCCTTAACAGCTTTAAGTTAGGTCCCGCTGATGAGTACATAGTAAACCAAAATGACTTAGGAGTAGCCTTAGACACGATTAAGGCCGAACTCAAAGATCGCTTAAAGTACTTTGAACGGTTAAACTTCCCCGAACGTGCCCAGCGCTTACAAACGATTACCGAACACGATTTAGCAGATCTGAAGGCCTGGGGGGTATGTAGTGGCGTGGAAAACTATGCGCGGCATTTAGAACACCGCCCACCGCACTCCAAGCCGTATAACATCTTTGATTACTTTACTAAAGGTGAATGGCTATTGGTAGTTGATGAATCCCACCAAACGCTACCGCAAATTAAGGGGATGTACAACACCGATATTTCACGGAAACAAAGCCTAATTGAGTACGGTTTTCGCCTCCCTTCAGCTTTAGACAACCGTCCTTTATCCTATGAGGAATTCCGCCAGGGAATCAACAAGGTCATTTATGTATCCGCTACCCCTAGGGAAGAGGAAATTCAGTTAAGTCACAACAACGTGGTCGAACAGTTAGTGCGTCCCACCTACCTCTTGGACCCGGAAGTGATTGTGAAACCGAAGGACAACCAGGTTGAAGACCTAGTGAGTGAAATCATTAACCAAAGAAAACACAACGGTCGTACCTTTGTCACGGTGTTAACGATTAAGATGGCGGAAAACCTGACTGACTTTTTGAAGGAGCGCAACATTAAGGTAGCGTACATCCACAAGGATATTAAAGCCTTGGAACGTTTAATTCTGTTAACGGATTTACGCAAAGGTGAGTATGAGTGTTTAGTGGGGATTAACTTACTGCGAGAAGGTTTAGATGTTCCTGAAGTTTCACTCGTAGCCATCTTTGACGCTGACATTCCTGGATTACCACGCGATGAGCGCAGTTTAATCCAGATAATTGGCCGCGCTGCGCGAAACGTTCATGGCCGGGTGATTATGTATGCCAATACCATTAGCGAACAAATGGATAAGGCGATTAAGGAAACGCAACGCCGGCGCACCATCCAAATGGCTTACAACGAACAACACCACAAAACACCAATGACGGTGCAAAAACCAATTACCTTAAACCAGCCGATTAAGTTAAAGACCAAAAGCTCAGAGCAGCAAAAAGCTGCTTTAATTAAGCAGCTAACCAAGGAAATGAAGCAAGCAGCCGCTAACCAAAACTATGAGTTAGCGATTGAGATCCGCGACTCGATCTTTGAATTGGAAAAGCAATTTCGTGGTAAAATTAAGAGCTAG", "aa": "MKSPTKDSKLFHLKSNFAPTGDQPAAIAKLAEFQTNEQVLLGATGTGKTFTIANVIQKVQLPTVVIAHNKTLAGQLYQELKELFPNNAVEYFISYFDFYQPEAYLPAKGVYIEKSATVNEEIKRLRVSTLHSLSTRKDVIVVGSVASIYPTSSPADFAQYSLWLVVGKEYGLSELKTQLIHLNYVVNKQQLTPGKFRFQGDVVEVFPGYAQDYVLRLSFFDQQLEQIARIDPLTNKVLETLNSFKLGPADEYIVNQNDLGVALDTIKAELKDRLKYFERLNFPERAQRLQTITEHDLADLKAWGVCSGVENYARHLEHRPPHSKPYNIFDYFTKGEWLLVVDESHQTLPQIKGMYNTDISRKQSLIEYGFRLPSALDNRPLSYEEFRQGINKVIYVSATPREEEIQLSHNNVVEQLVRPTYLLDPEVIVKPKDNQVEDLVSEIINQRKHNGRTFVTVLTIKMAENLTDFLKERNIKVAYIHKDIKALERLILLTDLRKGEYECLVGINLLREGLDVPEVSLVAIFDADIPGLPRDERSLIQIIGRAARNVHGRVIMYANTISEQMDKAIKETQRRRTIQMAYNEQHHKTPMTVQKPITLNQPIKLKTKSSEQQKAALIKQLTKEMKQAAANQNYELAIEIRDSIFELEKQFRGKIKS", "aa_hexdigest": "75e1c30c7f4ab8519fd501ad689d7074", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137EBA", "ncbi_nrp_id": "WP_010874568.1", "uniref100_id": "UniRef100_P75558", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137EBA", "RefSeq:WP_010874568.1", "UniRef:UniRef100_P75558" ] }, "ips": { "uniref100_id": "UniRef100_P75558", "uniref90_id": "UniRef90_P75558" }, "psc": { "uniref90_id": "UniRef90_P75558", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "uniref50_id": "UniRef50_P47319", "cog_id": "COG0556", "cog_category": "L", "kegg_orthology_id": "K03702", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47319", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47319" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "GIHJHMOALN_492", "locus": "GCA009939745_00457" }, { "type": "cds", "sequence": "contig_1", "start": 259902, "stop": 260309, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPNE_0244", "db_xrefs": [ "RefSeq:WP_010874569.1", "SO:0001217", "UniParc:UPI00001391FC", "UniRef:UniRef100_P0CJ80", "UniRef:UniRef50_P0CJ80", "UniRef:UniRef90_P0CJ80" ], "nt": "ATGCGTAAACTAATTAAATTAAACGTCATTGTCTTTGTCTTGTTGTACTTGGGCGAGCTGTTTGCCAGCCTTTCGTTCAAGTTAATCAGTTGCCTCAAGACACGCAACCAGTACTCCTTAAACGGGTACTATGCGTTGTTTGTCTTTGTCAACATCATCCAAAAGATGGCTAACTCTTTCCAAAAGTTAGCTTCCTCAGTTGTGTTGTTTGAAACTGAAATTAACGAATTTTTAGTTCTCTTTACTGATACAAAGAATAAGCGTGAGGAGAGTGAACCAGTGCGCCAGGTGTCAACAACCCAAGAGTATCACCAGGTTACGCTCGACCAACAACACTACTTTAACCACAAACTGAGCGATTACTTCCGTTTGTTTAAGGACAAAACTTTCTTCTTTGAAATTATCTAG", "aa": "MRKLIKLNVIVFVLLYLGELFASLSFKLISCLKTRNQYSLNGYYALFVFVNIIQKMANSFQKLASSVVLFETEINEFLVLFTDTKNKREESEPVRQVSTTQEYHQVTLDQQHYFNHKLSDYFRLFKDKTFFFEII", "aa_hexdigest": "7839f1432cde41d0c75f09a4d6153c07", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI00001391FC", "ncbi_nrp_id": "WP_010874569.1", "uniref100_id": "UniRef100_P0CJ80", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001391FC", "RefSeq:WP_010874569.1", "UniRef:UniRef100_P0CJ80" ] }, "ips": { "uniref100_id": "UniRef100_P0CJ80", "uniref90_id": "UniRef90_P0CJ80" }, "psc": { "uniref90_id": "UniRef90_P0CJ80", "product": "Uncharacterized protein MPNE_0244", "uniref50_id": "UniRef50_P0CJ80" }, "pscc": { "uniref50_id": "UniRef50_P0CJ80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0CJ80" ], "product": "Uncharacterized protein MG074 homolog" }, "genes": [], "id": "GIHJHMOALN_493", "locus": "GCA009939745_00458" }, { "type": "cds", "sequence": "contig_1", "start": 260414, "stop": 263302, "strand": "+", "frame": 2, "gene": null, "product": "putative MG075-like protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47321", "UniRef:UniRef90_P75556" ], "nt": "ATGAAGCTTAGTGCTATTATCTCCCTATCAGTCGCTGGTACTGTGGGAACAACTGCGGTGGTAGTACCTACAACTATAACGCTTGTAAATAAGACCCACCAAGTAGAACATGAATCAGAACAATCGGATTTTCAAGATATTCGCTTTGGTCTTAATAGTGTTAAGTTGCCAAAAGCACAGCCAGCTGCGGCAACTAGAATTACCGTGGAAAACGGGACTGATAAATTAGTCAACTATAAGTCCTCACCACAACAACTCTTTTTAGCGAAGAACGCGCTTAAGGATAAACTCCAAGGTGAGTTTGATAAATTCCTAAGTGATGCGAAGGCCTTCCCAGCGCTAACCGCTGATTTACAGGAATGGGTTGACCAACAGCTGTTTAATCCAAACCAAAGTTTCTTTGATTTAAGTGCGCCCAGGTCAAACTTTACCCTTTCATCTGACAAAAAGGCTAGTTTAGACTTTATCTTCCGCTTTACTAACTTCACCGAATCCGTTCAGTTGTTAAAACTACCAGAAGGTGTATCGGTTGTAGTTGACTCCAAACAAAGCTTTGATTACTATGTCAATGCTAGTGCCCAAAAATTATTAGTTCTACCGCTGTCTTTACCAGATTACACTTTGGGTTTAAACTATATGTTTGACCACATTACTTTAAACGGTAAGGTTGTCAATAAATTTAGTTTTAATCCGTTCAAAACGAATTTAAACCTCGCCTTTAGCAACGTTTACAATGGCGTTGATGTGTTTGAAGCACAAAAGAATTTAGTAGGTAAGGGTAAATACCTCAACACCCACGTGAAGGCTGAAGACGTAAAGAAGGACGTTAATGCCAACATTAAAAACCAATTTGACATTGCCAAAATTATCGCTGAGCTAATGGGTAAAGCCCTTAAAGAATTTGGCAATCAACAAGAAGGTCAACCATTATCCTTCCTAAAGGTAATGGATAAAGTTAAAGAAGATTTTGAAAAACTGTTTAACTTAGTCCGTCCTGGATTGGGTAAATTTGTTAAGGACTTAATCCAAAGTAGTAGTCAAGCAGAAAACAAGATAACTGTCTACAAGTTAATCTTTGACAACAAAAAGACCATCTTAAACCTACTTAAAGAGCTTTCCATTCCGGAATTAAACTCTTCTTTAGGTTTAGTGGACGTCTTGTTTGATGTCATTACTGACTCTGATGGTCTCTATGAAAGGTTGCAATCTTTCAAAGACTTAATCGTTCCAGCAGTTAAAACGAATGAAAAAACCGCGGCTTTAAGTCCATTAATTGAAGAGTTATTAACCCAAAAGGATACCTATGTGTTTGACTTAATTCAAAAACACAAGGGTATCTTGACTAACTTGTTAAAGAACTTCTTAGCTGATTTCCAAAAATCAACGCCGTTTATGGCTGATCAAGTAGCCATCTTCACTGAGTTATTTGACAACGAAGGTGCGTTTGATTTATTTGGTGAGGCTGACTTTGTTGACAAGATTGCCGAACTCTTCTTAACAAAGCGTACTGTTAAAAATGGTGAAAAAATTGAAACTAAAGATTCCCTACTGGTAACATCATTAAAGAGTCTTTTAGGGGAAAAGGTAGCTGCCTTAGATGATTTGTTAGATAGCTACATCTTTAAAAATGAATTACTTAACCGCAGTGTAGAAGTGGCTAAGGCTGAAGCTAAGGACACTAAAGGTGCTACCGATTACAAAAAGGAACAAGCTAAGGCGCTCAAAAAACTCTTTAAACACATTGGTGAAAACACTTTAAGTAAAACCAATCTCGATAAAATCACCTTAAAAGAAGTTAAAAACACCGAAAATGTTGAATTAGAAGAAACTGAAACAACCTTAAAGGTTAAAAAACTCGATGTTGAATACAAGGTAGAACTTGGCAACTTTGAAATCAAGAATGGCTTGATTAAGGCAATGCTCGAATTCTTGCCAGACCCTAAAGATTTAGAAACAACTTTAGATAAACTCTTGTTCAAAGGGGAAAGCTACAAAGCGATGAAAGACAAGTACATCAAGGAAGGTTTCCCTGGTTATGGTTGGGCTAAAGGAGTAGTCCCTGGGGCCTTTGAATCTATTGAAAATACTTTTAAGAGTGCTATTGATAAAACCAAGTCCATTCGTGATCTCTTTGGCGACATGCTCTTTGGTAACGATTTAAGCAGCGTTAAAGAAACTGATTCATTCATCACCCTAGGTGGTTCGTTCGACATTAAGTATGGCGGTGAAAATCTCAATGTGTTGCCAGCTTACTACTCTTTAATTAACAGCGAAATTGGCTATCAAATTATTGGTGTAGATACCACAATTGATGCAACTAAGGTAAAAGTTGAATTAAAAAACAAAGAGTATAAGGGTAAATCGCCAGCTATTAACGGTCAGGTGAAGTTGTCACAATCATTCTTTAATGTTTGGACAAATATGTTTGACAGCATTACCAAACAAATCTTCCAAAAGAAATACGAGTTCAAAGATAACATCCAAGTGTTTGCGCGAAACGAAGATAACACATCACGTTTAGAACTTGACATTTCTGATCCTGAACAACGGGTAATTCCATTTGCTTTTGTTGATGGCTTTGGCATTCAACTCAAAGCAGTTGACAAAAACATTACGAAAGAAGCAGGTAATACTGAGCCAAAATCTCCTGTAATTCAACTTTATGAGGCACTTAATAAAGAAAAAGATCAAAAACAACAAAGTAAACAATCTCCAAAACAACTTGATACTAAAACACAGTTGGGTTACCTATTGAAATTAGGCGACAATTGGAGTAAAGATGATTACAAAAGCTTAATTGATGATACGATCATCAATAACAACTATTTAGAGGCCAGCTTTAATTCCAAGATAACGGTTGATCGCTTGGGTATTCCTATTGACCTTTGA", "aa": "MKLSAIISLSVAGTVGTTAVVVPTTITLVNKTHQVEHESEQSDFQDIRFGLNSVKLPKAQPAAATRITVENGTDKLVNYKSSPQQLFLAKNALKDKLQGEFDKFLSDAKAFPALTADLQEWVDQQLFNPNQSFFDLSAPRSNFTLSSDKKASLDFIFRFTNFTESVQLLKLPEGVSVVVDSKQSFDYYVNASAQKLLVLPLSLPDYTLGLNYMFDHITLNGKVVNKFSFNPFKTNLNLAFSNVYNGVDVFEAQKNLVGKGKYLNTHVKAEDVKKDVNANIKNQFDIAKIIAELMGKALKEFGNQQEGQPLSFLKVMDKVKEDFEKLFNLVRPGLGKFVKDLIQSSSQAENKITVYKLIFDNKKTILNLLKELSIPELNSSLGLVDVLFDVITDSDGLYERLQSFKDLIVPAVKTNEKTAALSPLIEELLTQKDTYVFDLIQKHKGILTNLLKNFLADFQKSTPFMADQVAIFTELFDNEGAFDLFGEADFVDKIAELFLTKRTVKNGEKIETKDSLLVTSLKSLLGEKVAALDDLLDSYIFKNELLNRSVEVAKAEAKDTKGATDYKKEQAKALKKLFKHIGENTLSKTNLDKITLKEVKNTENVELEETETTLKVKKLDVEYKVELGNFEIKNGLIKAMLEFLPDPKDLETTLDKLLFKGESYKAMKDKYIKEGFPGYGWAKGVVPGAFESIENTFKSAIDKTKSIRDLFGDMLFGNDLSSVKETDSFITLGGSFDIKYGGENLNVLPAYYSLINSEIGYQIIGVDTTIDATKVKVELKNKEYKGKSPAINGQVKLSQSFFNVWTNMFDSITKQIFQKKYEFKDNIQVFARNEDNTSRLELDISDPEQRVIPFAFVDGFGIQLKAVDKNITKEAGNTEPKSPVIQLYEALNKEKDQKQQSKQSPKQLDTKTQLGYLLKLGDNWSKDDYKSLIDDTIINNNYLEASFNSKITVDRLGIPIDL", "aa_hexdigest": "d9efd22db196a3ee0a9a99b1dc873665", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75556", "query_cov": 1.0, "subject_cov": 0.9339805825242719, "identity": 0.997, "score": 1826.0, "evalue": 0.0, "valid": true, "product": "Uncharacterized protein MG075 homolog", "uniref50_id": "UniRef50_P47321", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47321" ], "product": "Uncharacterized protein MG075" }, "genes": [], "id": "GIHJHMOALN_494", "locus": "GCA009939745_00459" }, { "type": "cds", "sequence": "contig_1", "start": 263342, "stop": 263506, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGGTTCCTTACAACTTTACAGTAGTAGTGTTATCTTCCCATACGGTATTTATGACACCAGTGTTCAAGATGCTACGAAGATTGTGAAGCGTCTGAACTTTACTGACATGGGCTTCAAACTCAACGATCCAAAACCTAACTTCTGGTTCGTTGGTTTTTAG", "aa": "MQGSLQLYSSSVIFPYGIYDTSVQDATKIVKRLNFTDMGFKLNDPKPNFWFVGF", "aa_hexdigest": "b2df7e44a22662d28d9049daa275eeab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6213.053599999999, "isoelectric_point": 8.06275463104248 }, "id": "GIHJHMOALN_495", "locus": "GCA009939745_00460" }, { "type": "cds", "sequence": "contig_1", "start": 263503, "stop": 263736, "strand": "-", "frame": 1, "gene": null, "product": "MPN214 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47322", "UniRef:UniRef90_P75555" ], "nt": "GTGTGTCTCCAAGGTATAGTGCGCTTAAAGACCTGTGATGGAGCACGGTACGAAATTAGCAAGTTTAACCAATATCTAAAACTAGGTAGTATATACGCCAAGCCCAACATTAGTTTTGACGAGTATAAAGCAAAAAGTTCATCCTACCGTAAACAAACCCGCGGGTTTTGGTGGATGAACTTCTCGCTTTACCTTCTAGGTAGCTTAATCAGTATAGTAGTTAGTCTGCTCTAA", "aa": "MCLQGIVRLKTCDGARYEISKFNQYLKLGSIYAKPNISFDEYKAKSSSYRKQTRGFWWMNFSLYLLGSLISIVVSLL", "aa_hexdigest": "5d740eb08b7127b983a78fc504f4c845", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 263503, "stop": 263967, "observations": { "mutation": [ 263745 ], "stop": [ 263745 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75555", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.987, "score": 154.0, "evalue": 1.62e-46, "gene_start": 1, "gene_end": 77, "reference_start": 78, "reference_end": 154, "reference_sequence": "MLVVQNILQKNDTSKYMLGTQTTNSKPREYGGLIVSTIYIVLFFAILNLTVFFNKTNNINLILKNSCVVSFVVVWLLVCLQGIVRLKTCDGARYEISKFNQYLKLGSIYAKPNISFDEYKAKSSSYRKQTRGFWWMNFSLYLLGSLISIVVSLL" }, "paralog": false, "identity": 0.987012987012987, "score": 300.0, "evalue": 1.05e-106, "description": "Internal stop codon at 263745. Nonsense mutation around 263745.", "psc": { "uniref90_id": "UniRef90_P75555", "product": "MPN214 family protein", "uniref50_id": "UniRef50_P47322", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75555", "UniRef:UniRef50_P47322", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47322" ], "product": "Uncharacterized protein MG076" } }, "id": "GIHJHMOALN_496", "locus": "GCA009939745_00461" }, { "type": "cds", "sequence": "contig_1", "start": 263743, "stop": 263961, "strand": "-", "frame": 1, "gene": null, "product": "MPN214 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47322", "UniRef:UniRef90_P75555" ], "nt": "GTGGTTCAAAACATTCTACAAAAAAACGATACTAGTAAGTACATGTTAGGTACACAAACCACAAATTCGAAGCCGCGTGAATACGGCGGGTTAATAGTAAGCACAATTTACATTGTGCTTTTCTTCGCCATTTTGAATTTAACCGTTTTTTTCAATAAAACTAACAACATTAACCTAATTCTGAAAAACAGTTGTGTTGTTAGTTTTGTCGTTGTTTGA", "aa": "MVQNILQKNDTSKYMLGTQTTNSKPREYGGLIVSTIYIVLFFAILNLTVFFNKTNNINLILKNSCVVSFVVV", "aa_hexdigest": "35dc1714782f8651273b97459afd46a7", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 263503, "stop": 263967, "observations": { "mutation": [ 263745 ], "stop": [ 263745 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75555", "query_cov": 1.0, "subject_cov": 0.4675324675324675, "identity": 0.986, "score": 134.0, "evalue": 7.54e-39, "gene_start": 1, "gene_end": 72, "reference_start": 3, "reference_end": 74, "reference_sequence": "MLVVQNILQKNDTSKYMLGTQTTNSKPREYGGLIVSTIYIVLFFAILNLTVFFNKTNNINLILKNSCVVSFVVVWLLVCLQGIVRLKTCDGARYEISKFNQYLKLGSIYAKPNISFDEYKAKSSSYRKQTRGFWWMNFSLYLLGSLISIVVSLL" }, "paralog": false, "identity": 0.987012987012987, "score": 300.0, "evalue": 8.72e-107, "description": "Internal stop codon at 263745. Nonsense mutation around 263745.", "psc": { "uniref90_id": "UniRef90_P75555", "product": "MPN214 family protein", "uniref50_id": "UniRef50_P47322", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75555", "UniRef:UniRef50_P47322", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47322" ], "product": "Uncharacterized protein MG076" } }, "id": "GIHJHMOALN_497", "locus": "GCA009939745_00462" }, { "type": "cds", "sequence": "contig_1", "start": 264150, "stop": 264350, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCAAGTATATTATTAAACGTTTGGGTTTGGCAGTGTTAGCGATGTTTATCGTGATGACCATTGTCTTCTTTTTGGTTAACTCCACAGGTCAAACGCCCTTATCAGCAACCTCTTCTAAGGATTTGGAGGCTGTCAAAACACAACTAGATGCCTTTGGTTTTAACGATCCGTTAATTGTGCGATATGGTCGTTACTGA", "aa": "MFKYIIKRLGLAVLAMFIVMTIVFFLVNSTGQTPLSATSSKDLEAVKTQLDAFGFNDPLIVRYGRY", "aa_hexdigest": "3809ea6829e43cd6717152c73b74461a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7421.7404000000015, "isoelectric_point": 9.599229621887208 }, "id": "GIHJHMOALN_498", "locus": "GCA009939745_00463" }, { "type": "cds", "sequence": "contig_1", "start": 264372, "stop": 265112, "strand": "+", "frame": 3, "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "db_xrefs": [ "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75554", "UniRef:UniRef90_P75554" ], "nt": "TTGGGTACTTATTACAGCTCCCCGAACCAAACAATTGACCAAATTGTCTTTGGTCGGGTACCTAACACCCTTTACGTTGTTTTAATTTCTTTCTTTATCGGCTCTTTGTTGGGCATTATCTTCGGGATGATTTCCGGATTATTCCGTGGCAAGTTAATTGATGCGGTAATTAACGTGCTCGTTGTTTTATTTGTTTCGATTCCTTCCTTTGTGGTTGGTTTAGGTTTACTTAAGGCAGCTGGTTTGTTCCGTTTACCACCGCGCTTTATTAACTTCGATGATGCTAACTTTAACTTTGGTAACTTCTTGTTAGCCTCCATTATTCCTATTTTATCCTTGGTATTTTACACCTCAGCGGCCTTTACTTATCGGGTACGGAATGAGGTCGTGGAAGTAATGAACCAGGACTACATAAAAACAGCACGTAGTAAAGGTTTGAGTACTTTTGCTGTAGCGCTTTACCACATCTTCCGTAACTCAATTATTCCTTCTGTACCTTTATTTGTCTTTGGGATCTCTGGTGCCTTTTCGGGTGGTTTTATTATTGAATCCTTATTTGGTGTTCAGGGGGTATCACGGATCTTAATTGACTCGGTGCAAAGTAACGAAACTAACCTCGTGATGTTTAACATTATGTTCATCCAGGGGATTCCCTTACTAGCAAGTGTCTTCATTGAGTTGATTTATGTCCTAGTGGATCCTAGGATTCGGATTGCTAGTGCCGGTGGGGTGAGTCTGTGA", "aa": "MGTYYSSPNQTIDQIVFGRVPNTLYVVLISFFIGSLLGIIFGMISGLFRGKLIDAVINVLVVLFVSIPSFVVGLGLLKAAGLFRLPPRFINFDDANFNFGNFLLASIIPILSLVFYTSAAFTYRVRNEVVEVMNQDYIKTARSKGLSTFAVALYHIFRNSIIPSVPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQSNETNLVMFNIMFIQGIPLLASVFIELIYVLVDPRIRIASAGGVSL", "aa_hexdigest": "927a60e96aff7d810b1bbbb0bc289e61", "start_type": "TTG", "rbs_motif": null, "genes": [ "oppB" ], "truncated": "both", "pseudogene": { "start": 264150, "stop": 265364, "observations": { "mutation": [ 264348, 265110, 265146, 265158 ], "stop": [ 264348, 265110, 265146, 265158 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75554", "query_cov": 1.0, "subject_cov": 0.6089108910891089, "identity": 0.996, "score": 429.0, "evalue": 8.08e-149, "gene_start": 1, "gene_end": 246, "reference_start": 75, "reference_end": 320, "reference_sequence": "MFKYIIKRLGLAVLAMFIVMTIVFFLVNSTGQTPLSATSSKDLEAVKTQLDAFGFNDPLIVRYGRYWQTLFSGSLGTYYSSPNQTIDQIVFGRVPNTLYVVLISFFIGSLLGIIFGMISGLFRGKLIDAVINVLVVLFVSIPSFVVGLGLLKAAGLFRLPPRFINFDDANFNFGNFLLASIIPILSLVFYTSAAFTYRVRNEVVEVMNQDYIKTARSKGLSTFAVALYHIFRNSIIPSVPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQSNETNLVMFNIMFIQGIPLLASVFIELIYVLVDPRIRIASAGGVSLWTKLKFVYLRQAWLRKWRRINHTNSHNVLFNSPQHRQLLELKAIDYKHNTISLTEQQKTTLKIEPTANFVLLGTKCLKIITIHG" }, "paralog": false, "identity": 0.9876237623762376, "score": 732.0, "evalue": 1.38e-270, "description": "Internal stop codon at 264348, 265110, 265146, 265158. Nonsense mutation around 264348, 265110, 265146, 265158.", "psc": { "uniref90_id": "UniRef90_P75554", "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "uniref50_id": "UniRef50_P75554", "cog_id": "COG0601", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75554", "UniRef:UniRef50_P75554", "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75554", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75554" ], "product": "Oligopeptide transport system permease protein OppB" } }, "id": "GIHJHMOALN_499", "locus": "GCA009939745_00464" }, { "type": "cds", "sequence": "contig_1", "start": 265191, "stop": 265364, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGTTCAATTCGCCCCAACACCGCCAGCTGTTAGAGCTCAAAGCAATTGATTACAAGCACAATACCATTAGCTTAACTGAACAGCAAAAAACCACCCTTAAAATTGAACCAACTGCTAACTTTGTTTTGTTAGGAACTAAATGTTTAAAGATTATTACGATCCATGGATAA", "aa": "MLFNSPQHRQLLELKAIDYKHNTISLTEQQKTTLKIEPTANFVLLGTKCLKIITIHG", "aa_hexdigest": "19b9a9f4a652cc1778d49be5d039f633", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6532.632099999999, "isoelectric_point": 9.360825538635254 }, "id": "GIHJHMOALN_500", "locus": "GCA009939745_00465" }, { "type": "cds", "sequence": "contig_1", "start": 265357, "stop": 265494, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAGCACCAAAAATTTGACCAAAGCCTGTTTCAACGGGTAGACATTAATGTCTTAAAACGCTCTGACCAGTTGATTGGCAAACCAACCACTAACTTTGTCGAAATTATGAAGCGGTTGTTCCAAAATAAGTGA", "aa": "MDKHQKFDQSLFQRVDINVLKRSDQLIGKPTTNFVEIMKRLFQNK", "aa_hexdigest": "894d7b30ce7129b6cb20d3a36f168ca6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5436.275199999999, "isoelectric_point": 9.991906547546389 }, "id": "GIHJHMOALN_501", "locus": "GCA009939745_00466" }, { "type": "cds", "sequence": "contig_1", "start": 265519, "stop": 265842, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAATTATTTTGTTAGCCATCATTGTACCCTTAGCATCACCTTATTCAGCCGTTACTCCAGTATCGAACAACGCTTTGGCACAGAACCTACCACCACGATACCTCTGGAACGGCGCGGGTGACATTCAAGTAGAGAAAATTACGGCACGCTCGATTGCTGAAGTGGCCCAATCAAGTGGTGTTTTGGTAGGGAAATTACCGGAGGCAAGTAGCAATCCACTCGCTACTAACGTTAAGTACAACATTGCGCCTTACCAACTAGCAGAGCTGAAAAACTACTATCCATTATTAGGCACTAACGGTTTAGGGGTTGATATTTGA", "aa": "MLIILLAIIVPLASPYSAVTPVSNNALAQNLPPRYLWNGAGDIQVEKITARSIAEVAQSSGVLVGKLPEASSNPLATNVKYNIAPYQLAELKNYYPLLGTNGLGVDI", "aa_hexdigest": "8b299a0697ca8830e9c05c81a8acf52f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11331.995999999988, "isoelectric_point": 6.0894681930541985 }, "id": "GIHJHMOALN_502", "locus": "GCA009939745_00467" }, { "type": "cds", "sequence": "contig_1", "start": 265888, "stop": 266115, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTAGCCTTAGTTTCAATGGTCTTCGGCACCATTTACGGCGCGATTGCTGGGAGCTTTGTTGGACGCGCTGCTGACAACATTATGAGCCGGATCATTGAAATTATTGATTTGGTTCCTTCAATTCTCTGGATCATTGTCTTGGGAGCAACATTCCGCTTTGGTGGGGTAAAACAATTTGATGACAGTGTGGTGATCTTTACCTTAATCTTTGTCTTTTGGACCTGA", "aa": "MVALVSMVFGTIYGAIAGSFVGRAADNIMSRIIEIIDLVPSILWIIVLGATFRFGGVKQFDDSVVIFTLIFVFWT", "aa_hexdigest": "47561f5ccf9a58cd93b6b075eb5b32ac", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8230.744499999997, "isoelectric_point": 4.777965354919433 }, "id": "GIHJHMOALN_503", "locus": "GCA009939745_00468" }, { "type": "cds", "sequence": "contig_1", "start": 266221, "stop": 266487, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACCAGTTGTAACTGGTCGTTTAGCAGTCGTCTTTGTGAGTTTGATTCCCGCTGTGATTGGTTATGAAGCATCACTGGTTTTCTTAGGGCTTAAACCTGCTACCGAAGTGGGACTTGGTGCTTTATTAAACCAGGTCACCAGTAGTGGTAACATAGCACTGATTACCAGCTCAATTGTGAGCTTTGCAATTCTCACCGTTTCGACCCGCGTGTTTGCTAACGCTTTAAATGACGCAATTGACCCACGGGTAATAAGGAGGTAA", "aa": "MLPVVTGRLAVVFVSLIPAVIGYEASLVFLGLKPATEVGLGALLNQVTSSGNIALITSSIVSFAILTVSTRVFANALNDAIDPRVIRR", "aa_hexdigest": "5315e020ae5c14c46bf1431cf9d7c664", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9179.767299999989, "isoelectric_point": 9.961541938781735 }, "id": "GIHJHMOALN_504", "locus": "GCA009939745_00469" }, { "type": "cds", "sequence": "contig_1", "start": 266489, "stop": 266788, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGCACTAAAAGCAACTAATTTTTTTACAGAACCAGAGTACAAACTGCAGGATGATTTGATCTTAGACATCCGTGATCTTCACGTGAGTTTTAAGGTTAAAGACGGCATTATGCAAGCTGTCCGTGGTGTTGATCTCAAAGTCAAAAAGGGCGCCATTGTCGGCATTGTGGGCGAATCTGGTAGTGGTAAATCGGTCTGTGTTAAGTCGATTATTGGCTTTAACGATGGCGCAAAAACGACCGCTAAGTTAATGAACTTTAAGAACATTGACATTAGCAAGATTAAAAAGCACCAGTGA", "aa": "MALKATNFFTEPEYKLQDDLILDIRDLHVSFKVKDGIMQAVRGVDLKVKKGAIVGIVGESGSGKSVCVKSIIGFNDGAKTTAKLMNFKNIDISKIKKHQ", "aa_hexdigest": "89a6349195c0e7dc09fdebe82169a6d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 42, "aa_cov": 0.42424242424242425, "hmm_cov": 1.0, "evalue": 2.2499753892513962e-09, "score": 33.50126647949219, "start": 42, "stop": 83 } ], "seq_stats": { "molecular_weight": 10851.64399999999, "isoelectric_point": 9.553457069396973 }, "id": "GIHJHMOALN_505", "locus": "GCA009939745_00470" }, { "type": "cds", "sequence": "contig_1", "start": 266810, "stop": 267760, "strand": "+", "frame": 2, "gene": "oppD", "product": "Oligopeptide transport ATP-binding protein OppD", "db_xrefs": [ "COG:COG0444", "COG:EP", "EC:7.4.2.6", "GO:0005524", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75552", "UniRef:UniRef90_P75552" ], "nt": "GTGTCTTACATTTCCCAAGATCCACTGTTCTCGTTAAACCCCACCATGACCATCGGTCGCCAGGTTAAAGAAGCGATTTATGTGTCGTGTAAGCGCCGATATTACCAAACTAAGAGTGACCTCAAATACGATTTGCAAACAGAACGAATTGACCTGGACACTTACAAGGAAAAACTAGCACAAGCCAAGGAAACTTACAAAGCTAAAACGACTAAGGCGGCGGTCCATGCAAAAACGCTAGAAATCCTTAACTTCATTGGCATTGACCAAGCTGAAAAGCGCTTAAAGGCCTTTCCAAGTGAGTTTTCCGGGGGAATGCGGCAACGGATTGTGATCGCGATTGCCGTAGCCACCGAACCGGATTTAATTATTGCCGATGAACCGACCACGGCGTTGGATGTGACCATCCAAGCGAAGGCGTTGAACCTAATTAAACAGCTGCGTGACCTGCTCAACATTACCATTATTTTTATTAGTCACAACATCTCGTTAATTGCTAATTTCTGTGACTTTGTATACGTAATGTACGCTGGTCGATTAGTGGAAAAGGGCTTAGTTGAGGAAATCTTTACCAATCCGGTGCACCCCTACACTTGGGCATTAATGGCTTCTATCCCTGAAGGTACGGACAAAAACGCTCCGCTAACCTCAATTCCTGGGTATATTCCCAATATGTTATCCCCGCCTAAGGGCGATGCCTTTGCAGCGCGGAACCAGTTTGCACTTGCCATTGATTTCGAGCACCAACCGCCGTTCTTTGATGTAACGGAAACTCATAAGGCCGCTACCTGGCTACTCCATCCCCAAGCACCGAAGGTAGAGATGCCCGCCGATGTCAAGGAAAAGATTGCCATTACCCGCAAAGCACTCGCCATTAAAATGCCATCTCAACCTGTAAAACAACCAAAAAGCAATGGAAACAAAAAAACAAAAACAAAATCCGCTCGTTAA", "aa": "MSYISQDPLFSLNPTMTIGRQVKEAIYVSCKRRYYQTKSDLKYDLQTERIDLDTYKEKLAQAKETYKAKTTKAAVHAKTLEILNFIGIDQAEKRLKAFPSEFSGGMRQRIVIAIAVATEPDLIIADEPTTALDVTIQAKALNLIKQLRDLLNITIIFISHNISLIANFCDFVYVMYAGRLVEKGLVEEIFTNPVHPYTWALMASIPEGTDKNAPLTSIPGYIPNMLSPPKGDAFAARNQFALAIDFEHQPPFFDVTETHKAATWLLHPQAPKVEMPADVKEKIAITRKALAIKMPSQPVKQPKSNGNKKTKTKSAR", "aa_hexdigest": "aae043a35d3fe6f11b8df9911628f209", "start_type": "GTG", "rbs_motif": null, "genes": [ "oppD" ], "truncated": "5-prime", "pseudogene": { "start": 266510, "stop": 267760, "observations": { "mutation": [ 266786 ], "stop": [ 266786 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75552", "query_cov": 1.0, "subject_cov": 0.7470449172576832, "identity": 0.997, "score": 603.0, "evalue": 4.45e-216, "gene_start": 1, "gene_end": 316, "reference_start": 108, "reference_end": 423, "reference_sequence": "MALKATNFFTEPEYKLQDDLILDIRDLHVSFKVKDGIMQAVRGVDLKVKKGSIVGIVGESGSGKSVCVKSIIGFNDGAKTTAKLMNFKNIDISKMKKHQWQYYRGTYVSYISQDPLFSLNPTMTIGRQVKEAIYVSCKRRYYQTKSDLKYDLQTERIDLDTYKEKLAQAKETYKAKTTKAAVHAKTLEILNFIGIDQAEKRLKAFPSEFSGGMRQRIVIAIAVATEPDLIIADEPTTALDVTIQAKALNLIKQLRDLLNITIIFISHNISLIANFCDFVYVMYAGRLVEKGLVEEIFTNPVHPYTWALMASIPEGTDKNAPLTSIPGYIPNMLSPPKGDAFAARNQFALAIDFEHQPPFFDVTETHKAATWLLHPQAPKVEMPADVKEKIAITRKALAIKMPSQPVKQPKSNGNKKTKTKSAR" }, "paralog": false, "identity": 0.9927884615384616, "score": 817.0, "evalue": 1.37e-302, "description": "Internal stop codon at 266786. Nonsense mutation around 266786.", "psc": { "uniref90_id": "UniRef90_P75552", "gene": "oppD", "product": "Oligopeptide transport ATP-binding protein OppD", "ec_ids": [ "7.4.2.6" ], "uniref50_id": "UniRef50_P75552", "cog_id": "COG0444", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75552", "EC:7.4.2.6", "UniRef:UniRef50_P75552", "COG:COG0444", "COG:EP", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75552", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75552" ], "product": "Oligopeptide transport ATP-binding protein OppD" } }, "id": "GIHJHMOALN_506", "locus": "GCA009939745_00471" }, { "type": "cds", "sequence": "contig_1", "start": 267723, "stop": 268520, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGAAACAAAAAAACAAAAACAAAATCCGCTCGTTAATGTTAAAGCCTTGAGCATGTTGTTCAAGGTCCGCGGTAGCTTCTTTAAAGCCTTAGATGAAATTGACTTTACCGTCAATGAAGGTGACTTCTTTGGGGTCATTGGCGAATCGGGTAGTGGTAAGTCAACCACGGGGAAGTGTTTAATCCGGTTAAATATCCCGAGTGGCGGTAAGGTGGAAATTGCTAACCACCTTATTTCTGGTAAAAAACTAACGCGCGAAAACGACCACTGGTTAAAGCAAAACGTGCAGATGGTCTTTCAAGACCCATACTCTTCTTTAAACCCTACCAAGAATGTTTTAACGGTCATTTCCGAACCGTTAGTAATTACCAAAACGGTGTATGGTGAGGTAAAGGAATATCTTAAAACGCTCGCTAAACTGTCCTTTAAAACCAAGAAGGAATTGTTAAGGGAAGACTTTGAACTGGAAACCCAGTTTTACGAAAAGTTCTTTTCAAAGGTACTGTTTCACTTAGAAACTACGATTAACAAGTTTGCCCTACTCCAAGAGAGTAATAACAATAGTTCAGCCGAGTTAGCACAAACCATTTTGGGTCACACTGATGATCTAATTGAAGCATTACGGCAAGAGTTCGGTCTGGTCTATGAGTTTTCCTCATCCCAAAGCGAACCGCTACAAAAGGCCCTCAAAGACAAACAGGAAACCTTAGCACAGGACACGATTGACAAGTTAAAGCAAGAGCTGTATACAACGCAGCAAAAAGCTAAAGTTTCCACCCAGGCCTTTGCTACTTGA", "aa": "METKKQKQNPLVNVKALSMLFKVRGSFFKALDEIDFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKVEIANHLISGKKLTRENDHWLKQNVQMVFQDPYSSLNPTKNVLTVISEPLVITKTVYGEVKEYLKTLAKLSFKTKKELLREDFELETQFYEKFFSKVLFHLETTINKFALLQESNNNSSAELAQTILGHTDDLIEALRQEFGLVYEFSSSQSEPLQKALKDKQETLAQDTIDKLKQELYTTQQKAKVSTQAFAT", "aa_hexdigest": "03505af828e34312a190c749216153ea", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 111, "aa_cov": 0.4188679245283019, "hmm_cov": 0.8558558558558559, "evalue": 8.616961717511182e-16, "score": 54.283424377441406, "start": 31, "stop": 141 } ], "seq_stats": { "molecular_weight": 30032.951200000047, "isoelectric_point": 7.727852058410644 }, "id": "GIHJHMOALN_507", "locus": "GCA009939745_00472" }, { "type": "cds", "sequence": "contig_1", "start": 268527, "stop": 269516, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACAAACTAAGCAGAACTTAAAAGCGTACCGTGCCCAAATGGCGGAAGAGTTGCAAAACAAACCACGGATTTACCTCAATGCATGGTTGTTAACAACGAAGAACTACATTAAGGATTCACGCCAAAACACGCAGTTAACTGACGATGTCTTTGCTTTTTCTTACAACGACATGGTCGACAAAAAGCGCCGCTTAGTGTTAGTGTTATCGGAGTATTACAAGGCGTTGCCGTTCTTTTATGACAATTGGATCCACCAGAACGCCGATCGTTTTGATGAATTGACCAACGCAGTCTTCTTTGACTTGATTGATGTGGTGATTGCCTTAAACCGTGATTTTGCTAATGTGGAGAGTGACGCTAAGGCCGAGTTAATCCGTTTTGTTCAGTTTATCCGTCGCTTGTGTGACTTACGCTTTGCTGCTTTGAAAAAGAGTTTTAAGAAGCAAACTAACTATAGTTTTGACTTTAACCGCGAAACCGAGTTGTTATACGCCAACAGCTGTTATGACATTAAGGAGCTCCCCCAAGTAATCCAACCATACTGGGAAAAGCTCTTTAGTGATGCTAACTATGACAAGATTGCCAAATCAGTGCAGGAACTCAACGATATTATTAGCACTGACATTGAAAAGGCATCGAATATAGCCAGTGAAATTAATACCAAGATTAGTAGTTTTAAAACTGAAATTGCGGAGTTAAAAGCTACCTTTAAAACAGAAAAAAAAGCAGAAGATCACAGTGCCCAAATTACTGGCTTAAAAACGCAAATTGCTGAAATTCAAACGCAAATCAAGCAGCAAAAACGCGAAGTCCAAAGTACCGAAAAGGCCGCTTTAAAACCAGTATTAAAGCAGTACAAGAGTGCGCTGCACCTCTATAAACGCTTTAAACAGTTGTTACGCCAATTTACCAAGCAGTTAAACCTCCTTGTCAAAAAACAGCAGGAATTAGAAAAGATTGAGGAAGGCTTGGATCTAACCATTTGA", "aa": "MQQTKQNLKAYRAQMAEELQNKPRIYLNAWLLTTKNYIKDSRQNTQLTDDVFAFSYNDMVDKKRRLVLVLSEYYKALPFFYDNWIHQNADRFDELTNAVFFDLIDVVIALNRDFANVESDAKAELIRFVQFIRRLCDLRFAALKKSFKKQTNYSFDFNRETELLYANSCYDIKELPQVIQPYWEKLFSDANYDKIAKSVQELNDIISTDIEKASNIASEINTKISSFKTEIAELKATFKTEKKAEDHSAQITGLKTQIAEIQTQIKQQKREVQSTEKAALKPVLKQYKSALHLYKRFKQLLRQFTKQLNLLVKKQQELEKIEEGLDLTI", "aa_hexdigest": "c948cd74efcfd32189b59f5096becb41", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38752.906800000026, "isoelectric_point": 9.032294654846194 }, "id": "GIHJHMOALN_508", "locus": "GCA009939745_00473" }, { "type": "cds", "sequence": "contig_1", "start": 269532, "stop": 269678, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCCACCCGGTCGAAGGTGATCTCAAGAGTGAACTCAAAACACGCTTAAAGTCGTTTGGGGTGATTAACTTTGAGTACAAACGGGCTGTGCGTGAATCCCGTGTCTTTCGCTTAGTCCACTTTGGTCATGACGTGATGAAGTGA", "aa": "MFHPVEGDLKSELKTRLKSFGVINFEYKRAVRESRVFRLVHFGHDVMK", "aa_hexdigest": "bb6849b501534a7bde8abeb0c0c6821b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5735.6476, "isoelectric_point": 9.986491203308105 }, "id": "GIHJHMOALN_509", "locus": "GCA009939745_00474" }, { "type": "cds", "sequence": "contig_1", "start": 269709, "stop": 270278, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "nt": "ATGCGTAACAAGGTGTATGAAGCATTAGACAGTGTGGGCCTCAAACGCGAGCACGCTTATCGCTATCCGCACGAGTTTTCTGGGGGTCAGCGGCAACGGATTGCGATTGCCCGCGCTTTAATTACCAAACCTAAGTTAATTATTGCAGATGAGCTTATTAGTGCTTTGGATGTATCCATTCAAGCGCAGGTGATTAACATCTTGAAGGACTTGGCTAAAAAACACAACTTAACGGTGCTCTTTATTGCCCACGACCTTTCGATGGTACAAACAGTGTGCAACCGTTTAATTATTATGCACCGCGGCAAAATTGTGGAACGGGGTAGTACGGATGAAATCTTCGCGCACCCGGTCCACCCTTACACCCGTTCCTTGATTAAGGCATCGCCAAAGTTGAGTAAGATTAACATTGACCTAGCCTCCTTTGACGAGAAGTTTACGTATGACAGTGATTATTCACTCACGAATATGCCAAGCTTCTTGAAGGTGCCAAATACCCAAGAACACGAGTTGTACTGTACTCAAGGCCAGTTTGACAGTTGGATCAAAGGAGCAAGTCGGATAAATTAA", "aa": "MRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSTDEIFAHPVHPYTRSLIKASPKLSKINIDLASFDEKFTYDSDYSLTNMPSFLKVPNTQEHELYCTQGQFDSWIKGASRIN", "aa_hexdigest": "f94b1f67f71eb4fdf6171244cd681cf4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PJB1", "query_cov": 0.9417989417989417, "subject_cov": 0.9888888888888889, "identity": 0.6459999999999999, "score": 241.0, "evalue": 1.35e-78, "valid": false, "product": "Phosphate ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A4R0XTG3" }, "pscc": { "uniref50_id": "UniRef50_A0A4R0XTG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "GIHJHMOALN_510", "locus": "GCA009939745_00475" }, { "type": "cds", "sequence": "contig_1", "start": 270334, "stop": 270747, "strand": "+", "frame": 1, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180", "KEGG:K02867", "RefSeq:WP_010874576.1", "SO:0001217", "UniParc:UPI0000165F90", "UniRef:UniRef100_P75550", "UniRef:UniRef50_P47327", "UniRef:UniRef90_P75550" ], "nt": "ATGGCAAAAAAGACTATTACAAGAATCGCTAAGATTAACCTCTTAGGCGGGCAAGCAAAGCCCGGTCCCGCACTCGCATCTGTTGGGATTAACATGGGTGAATTCACCAAACAATTTAACGAAAAAACCAAGGACAAACAGGGCGAAATGATCCCCTGTGTGATCACCGCTTACAACGATAAGTCGTTCGACTTTATCTTAAAAACTACCCCAGTTAGCATCTTGCTTAAGCAGGCTGCAAAACTGGAAAAGGGGGCGAAAAACGCCAAAACAATTGTGGGTAAGATTACGATGGCCAAGGCCAAGGAAATTGCGCAGTACAAGTTAGTGGATCTGAACGCTAACACCGTGGAAGCAGCCCTTAAAATGGTTTTAGGTACTGCTAAACAAATGGGAATCGAGGTGATCGAATAA", "aa": "MAKKTITRIAKINLLGGQAKPGPALASVGINMGEFTKQFNEKTKDKQGEMIPCVITAYNDKSFDFILKTTPVSILLKQAAKLEKGAKNAKTIVGKITMAKAKEIAQYKLVDLNANTVEAALKMVLGTAKQMGIEVIE", "aa_hexdigest": "053c87ebf539a426ad2f8de866f82280", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F90", "ncbi_nrp_id": "WP_010874576.1", "uniref100_id": "UniRef100_P75550", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F90", "RefSeq:WP_010874576.1", "UniRef:UniRef100_P75550" ] }, "ips": { "uniref100_id": "UniRef100_P75550", "uniref90_id": "UniRef90_P75550" }, "psc": { "uniref90_id": "UniRef90_P75550", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_P47327", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P47327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47327" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "GIHJHMOALN_511", "locus": "GCA009939745_00476" }, { "type": "cds", "sequence": "contig_1", "start": 270747, "stop": 271064, "strand": "+", "frame": 3, "gene": null, "product": "Large ribosomal subunit protein uL1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZQ2" ], "nt": "ATGGCTAAACTATCAAAGAAAATGAAGATAGCTGTTGGTTTGGTCGATAAAACCAAGCTGTACCCGCTCCAAGAAGCAGTTGATTTAGTCAAAAAAACTTCAATTACCAAATTTAATGGCTCAGTTGATATTGCCGTTAGCCTTAATTTAGACACCACCAAGGCGGAACAACAGCTCCGCGGTGCGATTGCTTTTCCTCACAGTGTTGGCAAACCAATCCGTATTCTAGCGATCACCGATGATGAAAAGGCGGCACTGGAAGCAGGTGCTGACTTTGTTGGTGGCATTGACAAGATCAATGACATTAAAAACGGTTGA", "aa": "MAKLSKKMKIAVGLVDKTKLYPLQEAVDLVKKTSITKFNGSVDIAVSLNLDTTKAEQQLRGAIAFPHSVGKPIRILAITDDEKAALEAGADFVGGIDKINDIKNG", "aa_hexdigest": "5b19d4bfed94ee26523fc0082d49746b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZZQ2", "query_cov": 1.0, "subject_cov": 0.8467741935483871, "identity": 0.514, "score": 101.0, "evalue": 1.38e-25, "valid": false, "product": "Large ribosomal subunit protein uL1", "uniref50_id": "UniRef50_A0A448ZZQ2", "kegg_orthology_id": "K02863" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZQ2" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [], "id": "GIHJHMOALN_512", "locus": "GCA009939745_00477" }, { "type": "cds", "sequence": "contig_1", "start": 271101, "stop": 271427, "strand": "+", "frame": 3, "gene": null, "product": "Ribosomal protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "nt": "ATGGCCGCATTAGGTAAACTCGGGAAGCTGTTAGGTACCAAGGGTTTAATGCCTAACCCAAAAACAGAAACCGTTACTGATGATGTTCCAGCTGCTGTACGCGCTTACAAGAAGGGGAAAAAGGAATACCGCGCTGACAGCTTTGGTAACATCCACATGTCCTTAGGCCGCGTGGACAGTGCGTCCAACCACTTGGTTGAAAACGCTTTGGCACTGCTTGATCTCATTAAATCACGTAAACCAGCAACCGTTAAAGGAATTTACATTAAGAACATTGCCTTGACCACTACCATGGGTCCGAGCTTAAAGGTTAAGCTCCCTGATTAA", "aa": "MAALGKLGKLLGTKGLMPNPKTETVTDDVPAAVRAYKKGKKEYRADSFGNIHMSLGRVDSASNHLVENALALLDLIKSRKPATVKGIYIKNIALTTTMGPSLKVKLPD", "aa_hexdigest": "58ffddf1d8b4f0081ae864d6799677f9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2P6FDS1", "query_cov": 0.9629629629629629, "subject_cov": 0.9629629629629629, "identity": 0.5770000000000001, "score": 117.0, "evalue": 4.06e-32, "valid": false, "product": "Ribosomal protein", "uniref50_id": "UniRef50_A0A2P6FDS1", "kegg_orthology_id": "K02863" }, "pscc": { "uniref50_id": "UniRef50_A0A2P6FDS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "product": "Ribosomal protein" }, "genes": [], "id": "GIHJHMOALN_513", "locus": "GCA009939745_00478" }, { "type": "cds", "sequence": "contig_1", "start": 271427, "stop": 271651, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01195.25" ], "nt": "ATGGACAAGTTGCGCCTCGTTGTTGGTTTAGGTAATTTAGGTAAGCAGTACGCTGAAACCCGTCATAACGCGGGGTTTAAGGTAATTGATCGTTTACTAAGCCTTTACCATGTCCAGTTAGAGGAACGCAACAACTTAGGTGAATTCATCCTTTTAAGAAAACATAAGGTAGTGTTAGCTAAGCCAAACACTTACATGAACCACAGTGGTAAGTTTGTTAAATGA", "aa": "MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLAKPNTYMNHSGKFVK", "aa_hexdigest": "1cbf33d032f4bf75a9e1bde68f8f53dd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01195.25", "name": "Pept_tRNA_hydro", "length": 71, "aa_cov": 0.9324324324324325, "hmm_cov": 1.0, "evalue": 2.0900241201327966e-24, "score": 81.8519287109375, "start": 6, "stop": 74 } ], "seq_stats": { "molecular_weight": 8560.959699999996, "isoelectric_point": 10.070106697082519 }, "id": "GIHJHMOALN_514", "locus": "GCA009939745_00479" }, { "type": "cds", "sequence": "contig_1", "start": 271688, "stop": 271993, "strand": "+", "frame": 2, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "GO:0005737", "GO:0006412", "SO:0001217", "UniRef:UniRef50_P47329", "UniRef:UniRef90_P78034" ], "nt": "ATGGTGGTTTATGATGAATTAGCCTTCCCGTTAGGTACTGTTCGACTAAAAATGCAGGGTTCAGCCAACAATCATAATGGCATAAAGTCAGTAATTGCGCATTTAAATACTGAACACTTCAACCGCCTCCGGTTCGGAATTAAGTCCGATAATACTAGTAACATTCTCCATGAAGTGGTAATGTCGGAGTTTACCCCAGCCGAGCGTAATTTACTTGAAACAGCGCTTACAAAAGCGATTGAAGCACTCAAGGGTTACATTGATGGTGTGACAATGCTTAAATTAATGGAAGTTTTCAATGCCTAA", "aa": "MVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHLNTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLETALTKAIEALKGYIDGVTMLKLMEVFNA", "aa_hexdigest": "15015e4edb697318eee9f8f9aaf981fa", "start_type": "ATG", "rbs_motif": null, "genes": [ "pth" ], "truncated": "5-prime", "pseudogene": { "start": 271427, "stop": 271993, "observations": { "mutation": [ 271649, 271664 ], "stop": [ 271649, 271664 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78034", "query_cov": 1.0, "subject_cov": 0.5372340425531915, "identity": 1.0, "score": 198.0, "evalue": 4.05e-63, "gene_start": 1, "gene_end": 101, "reference_start": 88, "reference_end": 188, "reference_sequence": "MDKLRLVVGLGNLGKQYAETRHNAGFKVIDRLLSLYHVQLEERNNLGEFILLRKHKVVLAKPNTYMNHSGKFVKWACQNWNIKPDKVMVVYDELAFPLGTVRLKMQGSANNHNGIKSVIAHLNTEHFNRLRFGIKSDNTSNILHEVVMSEFTPAERNLLETALTKAIEALKGYIDGVTMLKLMEVFNA" }, "paralog": false, "identity": 0.9893617021276596, "score": 367.0, "evalue": 2.83e-132, "description": "Internal stop codon at 271649, 271664. Nonsense mutation around 271649, 271664.", "psc": { "uniref90_id": "UniRef90_P78034", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_P47329", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045", "GO:0005737", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78034", "EC:3.1.1.29", "UniRef:UniRef50_P47329", "COG:COG0193", "COG:J", "GO:0004045", "GO:0005737", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P47329", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47329" ], "product": "Peptidyl-tRNA hydrolase" } }, "id": "GIHJHMOALN_515", "locus": "GCA009939745_00480" }, { "type": "cds", "sequence": "contig_1", "start": 271986, "stop": 272195, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAACACAATACATAGCTGGAGTATCAGGTGGTCCTGATTCAATGTTGTTGTTAAAACTGTACCACAAGAAGATTGCCTGTGTTGTACACGTTAACTACAATAAGCGGACTACTGCACTCCGCGACCAAAAAATGGTCGAAGAATACTGTAAACAGCTCAAAGTGCCCCTCATTGTGCACACGGTACCTGAAGATACCGTTTGA", "aa": "MPKTQYIAGVSGGPDSMLLLKLYHKKIACVVHVNYNKRTTALRDQKMVEEYCKQLKVPLIVHTVPEDTV", "aa_hexdigest": "59eb6744c086998d12ffa2eacba17473", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7841.224099999999, "isoelectric_point": 9.230728340148925 }, "id": "GIHJHMOALN_516", "locus": "GCA009939745_00481" }, { "type": "cds", "sequence": "contig_1", "start": 272322, "stop": 272420, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGTTGGACGCACGCAAGCGTGCCATGTATTATGGCATTAAAACACGTGGCGAATTGTATGGGATGAAGGTCTATCGTCCCTTTATTAAGTACTGA", "aa": "MQLDARKRAMYYGIKTRGELYGMKVYRPFIKY", "aa_hexdigest": "ced749f3222818e496a77cb11ec9c2b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3947.6959000000015, "isoelectric_point": 10.087642097473143 }, "id": "GIHJHMOALN_517", "locus": "GCA009939745_00482" }, { "type": "cds", "sequence": "contig_1", "start": 272577, "stop": 272855, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCGCGTCATTGGTCAAAAGCTCTTTTCCTTAATGAAACGTGCAAAACAGTGGTTAGCTCATCCTGATGTACGTGAATTAAAGCGTTACCCACTTCCCGATCAACGTCAGTTGGTTTACAGCTTTTTAATTACCCACCACATTGATGTAACGGGGGACAAGATTGATGCCATTTTAGACTTTATCCAACCGTTTCAACAAAAGCATTACCGTCTCAAGGATGAGATCTTCTTATCCATTAAAGACGAACGGCTAACTTTACTGTACAAGAGTTAG", "aa": "MNRVIGQKLFSLMKRAKQWLAHPDVRELKRYPLPDQRQLVYSFLITHHIDVTGDKIDAILDFIQPFQQKHYRLKDEIFLSIKDERLTLLYKS", "aa_hexdigest": "e986e4fbe5ec5270d2f8cd4b97801e66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11109.88659999999, "isoelectric_point": 9.643519401550293 }, "id": "GIHJHMOALN_518", "locus": "GCA009939745_00483" }, { "type": "cds", "sequence": "contig_1", "start": 272856, "stop": 273794, "strand": "+", "frame": 3, "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "db_xrefs": [ "COG:COG1493", "COG:T", "EC:2.7.11.-", "EC:2.7.4.-", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109", "KEGG:K06023", "RefSeq:WP_014325409.1", "SO:0001217", "UniParc:UPI00023860AF", "UniRef:UniRef100_A0AB33HMD9", "UniRef:UniRef50_P75548", "UniRef:UniRef90_P75548" ], "nt": "ATGAAAAAGTTATTAGTCAAGGAGTTAATCGAACAATTTCAGGATTGTGTTAACCTCATTGATGGGCACACTAACACCAGTAATGTAATTAGAGTACCCGGATTAAAGCGGGTGGTGTTTGAAATGCTGGGGTTGTTTTCCAGCCAAATTGGCTCGGTAGCCATTTTGGGCAAACGCGAATTTGGCTTTTTGAGTCAAAAAACGCTGGTTGAACAACAGCAAATTCTCCACAACTTACTCAAACTCAACCCACCAGCGATTATTTTGACCAAGTCCTTTACTGACCCCACGGTACTTTTACAGGTTAACCAGACTTATCAGGTACCGATCCTAAAGACCGATTTCTTTTCCACGGAACTTTCCTTTACGGTCGAAACCTACATTAACGAACAGTTTGCTACTGTTGCACAAATTCACGGCGTCTTGCTGGAAGTTTTTGGTGTAGGTGTCTTATTAACTGGTCGCAGTGGCATTGGTAAATCGGAGTGTGCCTTAGATCTAATTAACAAGAACCACCTCTTTGTCGGTGATGATGCGATTGAAATATACCGTTTGGGTAATCGCTTGTTTGGTCGCGCGCAGGAAGTAGCGAAAAAGTTTATGGAAATCCGTGGTCTTGGTATTATTAACGTCGAGCGCTTTTACGGATTGCAAATTACTAAACAGCGCACCGAAATCCAGTTAATAGTGAACTTACTGAGTTTAGAAAAACAAACGACCGTGACCTTTGAGCGTTTAGGTACCGAATTGAAAAAACAACGTTTACTCGGAGTTGATTTGAGCTTTTATGAAATTCCGATTTCCCCCGGGCGGAAGACGAGTGAAATCATTGAAAGCGCTGTAATTGATTTTAAACTTAAGCACAGTGGCTATAACTCTGCTTTAGACTTTATTGAAAACCAGAAGGCCATTTTAAAACGTAAAAAAGATGAATCCTAG", "aa": "MKKLLVKELIEQFQDCVNLIDGHTNTSNVIRVPGLKRVVFEMLGLFSSQIGSVAILGKREFGFLSQKTLVEQQQILHNLLKLNPPAIILTKSFTDPTVLLQVNQTYQVPILKTDFFSTELSFTVETYINEQFATVAQIHGVLLEVFGVGVLLTGRSGIGKSECALDLINKNHLFVGDDAIEIYRLGNRLFGRAQEVAKKFMEIRGLGIINVERFYGLQITKQRTEIQLIVNLLSLEKQTTVTFERLGTELKKQRLLGVDLSFYEIPISPGRKTSEIIESAVIDFKLKHSGYNSALDFIENQKAILKRKKDES", "aa_hexdigest": "79cc13f76fab6f16cbca7453fa38b673", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00023860AF", "ncbi_nrp_id": "WP_014325409.1", "uniref100_id": "UniRef100_A0AB33HMD9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00023860AF", "RefSeq:WP_014325409.1", "UniRef:UniRef100_A0AB33HMD9" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HMD9", "uniref90_id": "UniRef90_P75548" }, "psc": { "uniref90_id": "UniRef90_P75548", "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "ec_ids": [ "2.7.11.-", "2.7.4.-" ], "uniref50_id": "UniRef50_P75548", "cog_id": "COG1493", "cog_category": "T", "kegg_orthology_id": "K06023", "go_ids": [ "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0006109" ] }, "pscc": { "uniref50_id": "UniRef50_P75548", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75548" ], "product": "HPr kinase/phosphorylase" }, "genes": [ "hprK" ], "id": "GIHJHMOALN_519", "locus": "GCA009939745_00484" }, { "type": "cds", "sequence": "contig_1", "start": 273901, "stop": 274713, "strand": "+", "frame": 1, "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "db_xrefs": [ "COG:COG0682", "COG:M", "EC:2.5.1.145", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158", "SO:0001217", "UniRef:UniRef50_P47332", "UniRef:UniRef90_P75547" ], "nt": "ATGTTTGTCTTGAAGTTAATTTACTTTTACAAGATTCAAGATAACAGCTTTTACTTCTTTATCTTCATTGCAGTTTTAACAATGGTATTGGGCGCACGAGCTTGGTATTTTTTGATTGAAGCGGTTGATGGTCGTTCAAGTGGGAGCAACTTCTTTGACTTCCGCAACGGTGGGTTAGCAATCCAAGGTGGTGTGTTGTTAACTACGTTAGCGGGGATTATCTACTTTAACGTCTTTTTAAACATGAAGACGACCAAAACCAAAACCACCGCTAAACTATTAAACAACAAGAACCAGATTAAAACGGTTTATGTCGAGCGTAACATCTCAGTGTTTGTGATGTTGGATCTCATCGCTCCCTGTGTGTTAATTGGTCAAGCCATTGGTCGTTGGGGTAACTTCTTTAACGCGGAAGTTTATGGCGCAGCTTTAGTGGGTTCCAAAAATGATACCCTGTCAGCTGCTAACACCACCTGGGGCTTTTTAAGAATCTTAATGCCCAAAGTGTGGGACGGAATGTTTATCAATGGTAGCTTCCGCATTCCGCTTTTCTTAATCGAATCGTTCTTTAATACGATCTTCTTTGTCTTTATCTACTTTGTAATGGACCACATTAAGGGGATCCGCAGTGGGACAATTGGCTTTAGTTACTTCTTAGCGACTGGCATAGTTCGCTTAATCTTGGAAACGCAGCGTGATGAAGCGTTTAAGTACAATACCTCGATTGTGTTTAGTGCACTCTTAATTTTGGTGGGAATAGTAGGGATTATTTACTGTCAAACGTTAGCAATTAAATTGCGTGGTTATTTCTGA", "aa": "MFVLKLIYFYKIQDNSFYFFIFIAVLTMVLGARAWYFLIEAVDGRSSGSNFFDFRNGGLAIQGGVLLTTLAGIIYFNVFLNMKTTKTKTTAKLLNNKNQIKTVYVERNISVFVMLDLIAPCVLIGQAIGRWGNFFNAEVYGAALVGSKNDTLSAANTTWGFLRILMPKVWDGMFINGSFRIPLFLIESFFNTIFFVFIYFVMDHIKGIRSGTIGFSYFLATGIVRLILETQRDEAFKYNTSIVFSALLILVGIVGIIYCQTLAIKLRGYF", "aa_hexdigest": "d809685d1086983575577ed9ac438c26", "start_type": "ATG", "rbs_motif": null, "genes": [ "lgt" ], "truncated": "both", "pseudogene": { "start": 273784, "stop": 274953, "observations": { "mutation": [ 273814, 274711 ], "stop": [ 273814, 274711 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75547", "query_cov": 1.0, "subject_cov": 0.6940874035989717, "identity": 1.0, "score": 521.0, "evalue": 7.79e-185, "gene_start": 1, "gene_end": 270, "reference_start": 40, "reference_end": 309, "reference_sequence": "MNPSVSSRPPWSTAFYLGPGFPIQWYGIIVAIGIAFGILMFVLKLIYFYKIQDNSFYFFIFIAVLTMVLGARAWYFLIEAVDGRSSGSNFFDFRNGGLAIQGGVLLTTLAGIIYFNVFLNMKTTKTKTTAKLLNNKNQIKTVYVERNISVFVMLDLIAPCVLIGQAIGRWGNFFNAEVYGAALVGSKNDTLSAANTTWGFLRILMPKVWDGMFINGSFRIPLFLIESFFNTIFFVFIYFVMDHIKGIRSGTIGFSYFLATGIVRLILETQRDEAFKYNTSIVFSALLILVGIVGIIYCQTLAIKLRGYFWTYFFLYGWYKVAAFFTTLFMKDRTQACSSKFAFYEKSLPEKERSFFQLKYYNDVLPPKIYRLYDHEMLMFDKLEAVPEA" }, "paralog": false, "identity": 0.9948586118251928, "score": 775.0, "evalue": 1.75e-287, "description": "Internal stop codon at 273814, 274711. Nonsense mutation around 273814, 274711.", "psc": { "uniref90_id": "UniRef90_P75547", "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "ec_ids": [ "2.5.1.145" ], "uniref50_id": "UniRef50_P47332", "cog_id": "COG0682", "cog_category": "M", "go_ids": [ "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75547", "EC:2.5.1.145", "UniRef:UniRef50_P47332", "COG:COG0682", "COG:M", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ] }, "pscc": { "uniref50_id": "UniRef50_P47332", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47332" ], "product": "Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase" } }, "id": "GIHJHMOALN_520", "locus": "GCA009939745_00485" }, { "type": "cds", "sequence": "contig_1", "start": 274771, "stop": 274953, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGATAGGACACAGGCTTGTTCTAGTAAGTTTGCATTTTACGAAAAGAGCTTACCTGAAAAGGAACGTTCCTTCTTTCAGTTGAAGTACTACAACGATGTTTTACCACCGAAAATTTACCGTTTGTACGACCATGAAATGCTCATGTTTGACAAGCTAGAAGCAGTGCCGGAAGCTTAG", "aa": "MKDRTQACSSKFAFYEKSLPEKERSFFQLKYYNDVLPPKIYRLYDHEMLMFDKLEAVPEA", "aa_hexdigest": "6ce78c1ca986456bc495a774dc51c15c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7287.329, "isoelectric_point": 6.553331184387207 }, "id": "GIHJHMOALN_521", "locus": "GCA009939745_00486" }, { "type": "cds", "sequence": "contig_1", "start": 274982, "stop": 275401, "strand": "+", "frame": 2, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02950", "RefSeq:WP_014325410.1", "SO:0001217", "UniParc:UPI0001DD64F2", "UniRef:UniRef100_A0AB38W7X5", "UniRef:UniRef50_P92532", "UniRef:UniRef90_P47333" ], "nt": "ATGGCTACAATCGCACAATTAATTAGAAAACCGCGGAAAAAGAAGAAGGTTAAATCTAAATCACCCGCTTTACATTACAACCTCAATTTACTCAACAAAAAGGTAACCAACGTGTATTCACCACTTAAACGTGGTGTGTGTACCCGGGTTGGTACCATGACTCCAAAAAAACCGAACTCTGCTTTGCGCAAATATGCCAAGGTAAGACTTACTAACGGTTTTGAGGTGTTGGCTTATATCCCTGGTGAAGGACACAACCTCCAAGAACACAGTGTAACGCTACTCCGTGGCGGTCGGGTGAAAGACCTCCCTGGTGTGCGTTACCACATTGTTCGTGGTACCTTAGACACTGTTGGTGTGGAAAAACGCCGTCAACAACGTTCAGCTTACGGGGCTAAGAAACCAAAAGCCAAGTCCTAA", "aa": "MATIAQLIRKPRKKKKVKSKSPALHYNLNLLNKKVTNVYSPLKRGVCTRVGTMTPKKPNSALRKYAKVRLTNGFEVLAYIPGEGHNLQEHSVTLLRGGRVKDLPGVRYHIVRGTLDTVGVEKRRQQRSAYGAKKPKAKS", "aa_hexdigest": "a3ad0284a3ab459e11c3b8aec2ce0e50", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64F2", "ncbi_nrp_id": "WP_014325410.1", "uniref100_id": "UniRef100_A0AB38W7X5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64F2", "RefSeq:WP_014325410.1", "UniRef:UniRef100_A0AB38W7X5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7X5", "uniref90_id": "UniRef90_P47333" }, "psc": { "uniref90_id": "UniRef90_P47333", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "genes": [ "rpsL" ], "id": "GIHJHMOALN_522", "locus": "GCA009939745_00487" }, { "type": "cds", "sequence": "contig_1", "start": 275455, "stop": 275760, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "nt": "ATGCGAAAAAATCGTGCTCCCAAACGGACTGTTTTACCCGATCCCGTTTTTAACAATACGCTGGTAACCCGGATTATTAACGTCATCATGGAAGATGGCAAGAAGGGTTTAGCACAACGAATCTTATACGGTGCTTTTGATCTCATTGAACAACGCACTAAAGAAAAACCCTTAACCGTGTTTGAACGCGCTGTGGACAACGTGATGCCGCGCTTGGAACTCCGGGTACGGCGGATTGCGGGATCCAACTATCAGGTACCTACCGAAGTACCTCAAGACCGCAAGATTGCTCTAGCACTCCGTTGA", "aa": "MRKNRAPKRTVLPDPVFNNTLVTRIINVIMEDGKKGLAQRILYGAFDLIEQRTKEKPLTVFERAVDNVMPRLELRVRRIAGSNYQVPTEVPQDRKIALALR", "aa_hexdigest": "61a6be463fd1b42d2fdc4b4247f07a1e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A381KMK1", "query_cov": 0.9900990099009901, "subject_cov": 0.8264462809917356, "identity": 0.52, "score": 113.0, "evalue": 1.5e-30, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A381KMK1", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A381KMK1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "product": "Ribosomal protein S7" }, "genes": [], "id": "GIHJHMOALN_523", "locus": "GCA009939745_00488" }, { "type": "cds", "sequence": "contig_1", "start": 275767, "stop": 275922, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00177.27" ], "nt": "ATGTTTGCGCGTAAGCGTCATGAAAAAACGATGTTAGAGAAGATTGCTAACGAAATTATTGATGCATCGAATAATACTGGTGCAGCCATTAAAAAGAAGGACGATACGCATAAGATGGCTGAAGCCAACAAGGCCTTTGCCCACATGCGTTGGTAA", "aa": "MFARKRHEKTMLEKIANEIIDASNNTGAAIKKKDDTHKMAEANKAFAHMRW", "aa_hexdigest": "7ac9d132e00cba7d2f89c12b1637683f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 0.8627450980392157, "subject_cov": 0.88, "identity": 0.75, "score": 72.8, "evalue": 4.05e-16, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00177.27", "name": "Ribosomal_S7", "length": 43, "aa_cov": 0.8431372549019608, "hmm_cov": 1.0, "evalue": 3.405736407289401e-18, "score": 61.14462661743164, "start": 2, "stop": 44 } ], "seq_stats": { "molecular_weight": 5899.768200000001, "isoelectric_point": 9.774519157409667 }, "id": "GIHJHMOALN_524", "locus": "GCA009939745_00489" }, { "type": "cds", "sequence": "contig_1", "start": 275945, "stop": 276304, "strand": "+", "frame": 2, "gene": null, "product": "Elongation factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2S3U2I5" ], "nt": "ATGGCGCGTACAGTAGATTTAATTAACTTCCGTAATTTCGGAATTATGGCCCACATTGATGCGGGTAAAACCACCACCTCAGAGCGAATTTTATTTCACTCAGGCCGAATCCACAAGATTGGGGAAACCCATGACGGGGAGTCCGTGATGGACTGGATGGAGCAGGAAAAGGAACGCGGTATTACGATTACCTCCGCAGCTACTTCAGTTAGTTGGAAGAACTGCAGCTTAAACCTAATTGACACCCCCGGTCACGTTGACTTTACCGTAGAAGTAGAACGCAGTCTCCGTGTCCTTGATGGTGCCATTGCTGTGTTAGATGCTCAAATGGGGGTAGAACCACAAACCGAAACGGTGTGA", "aa": "MARTVDLINFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERGITITSAATSVSWKNCSLNLIDTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETV", "aa_hexdigest": "66d3b63bb2431530da923cf91c9296ef", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2S3U2I5", "query_cov": 0.9663865546218487, "subject_cov": 0.8984375, "identity": 0.7909999999999999, "score": 184.0, "evalue": 2.62e-58, "valid": false, "product": "Elongation factor", "uniref50_id": "UniRef50_A0A2S3U2I5" }, "pscc": { "uniref50_id": "UniRef50_A0A2S3U2I5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2S3U2I5" ], "product": "Elongation factor" }, "genes": [], "id": "GIHJHMOALN_525", "locus": "GCA009939745_00490" }, { "type": "cds", "sequence": "contig_1", "start": 276326, "stop": 278011, "strand": "+", "frame": 2, "gene": null, "product": "Elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHN5" ], "nt": "GTGCCCCGGATTATCTTTGTCAATAAGATGGACAAAACGGGGGCTAACTTCGAGCGTTCTGTACAATCGATTCAACAGCGCTTAGGAGTAAAGGCGGTACCTATCCAATTACCAATTGGTGCGGAAAACGACTTTAACGGCATTATTGACCTGATTGAAGAGAAAGTTTACTTCTTTGATGGTGGTAAGGAAGAAAAGGCCGAGGAAAAACCAATTCCCGACCAGTTTAAGGATCAGGTCAAACAAATGCGTGCGCACCTAGTGGAAGAGGTGGCTAACTTTGACGATCAGTTAATGGCTGATTACCTTGAAGGTAAGGAAATTAGTATTGCCGACATCAAGCGCTGTATCCGCAAAGGGGTAATAGGTTGTCAGTTCTTCCCGGTGCTTTGTGGTTCTGCCTTTAAAAACAAGGGGATCAAACTGTTATTGGATGCTGTAGTAGATTTCTTACCTTCCCCAGTTGACGTACCGCAAGCCAAGGCTTATGGTGAAGATGGCAATGAGGTGTTAATCTCGGCTAGTGATGATGCACCGTTTGTTGGTTTAGCCTTTAAGGTAGCGACTGACCCATTTGTGGGACGTTTAACCTTTGTCCGCGTTTACTCCGGAGTACTTAAGTCCGGTTCTTACGTTAAGAATGTAAGAAAGAACAAAAAGGAACGGGTATCACGTTTGGTAAAAATGCACGCGCAGAACCGCAATGAAATCGAGGAAATTCGTGCTGGTGATATCTGTGCTATTATTGGATTAAAGGACACCACTACTGGAGAAACCCTAGTTGATGACAAGATTGATGTCCAACTAGAAGCGATGCAGTTCGCACAACCCGTGATCTCTTTAGCGGTGGAACCAAAAACCAAGGCAGATCAGGAAAAAATGTCGATTGCACTCTCGAAGTTAGCGGAAGAAGATCCGACCTTTAAGACCTTCACTGATCCCGAAACTGGGCAAACGATTATTGCGGGAATGGGTGAATTGCACCTAGACATCCTAGTGGATCGGATGCGCCGCGAGTTTAAGGTTGAAGTTAATGTGGGCGCACCACAAGTAAGCTTCCGTGAAACCTTCAATAAAGAAAGTGAAGTTGAGGGTAAATACATTAAACAATCGGGTGGCCGCGGTCAGTATGGTCATGTCAAGATTCGCTTTGAACCAAACAAGGACAAGGGCTTTGAGTTTGTCGACAAGATTGTTGGTGGTCGGATTCCGCGTGAGTACATTAAACCAGTGCAAGCGGGTTTGGAAAACGCTATGGCATCAGGTCCGCTAGCTGGTTATCCGATGATTGACATCAAAGCTACCTTGTTTGATGGTTCGTTCCACGAAGTTGATTCGAGTGAAATGGCCTTCAAGATTGCGGCATCCTTAGCTTTAAAAGAGGCGGGCAAGGTATGTAGTCCGGTACTGTTAGAGCCAATTATGGCAATTGAAGTGACGGTGCCAGAACAATACTTTGGGGACACCATGGGTGACATATCATCGAGAAGAGGTCTCATTGAGGGGACTGAACAACGTGATAACGTGCAGGTAATTAAGGCCAAAGTACCACTCAAAGAGATGTTTGGTTACGCCACTGATTTACGTTCCTTCTCTCAAGGTCGGGGTAACTATGTGATGCAGTTTAGCCATTACGCTGAAACCCCTAAGAGTGTGGTGAATGAGATAATTGCCACCAAGAAATAG", "aa": "MPRIIFVNKMDKTGANFERSVQSIQQRLGVKAVPIQLPIGAENDFNGIIDLIEEKVYFFDGGKEEKAEEKPIPDQFKDQVKQMRAHLVEEVANFDDQLMADYLEGKEISIADIKRCIRKGVIGCQFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPQAKAYGEDGNEVLISASDDAPFVGLAFKVATDPFVGRLTFVRVYSGVLKSGSYVKNVRKNKKERVSRLVKMHAQNRNEIEEIRAGDICAIIGLKDTTTGETLVDDKIDVQLEAMQFAQPVISLAVEPKTKADQEKMSIALSKLAEEDPTFKTFTDPETGQTIIAGMGELHLDILVDRMRREFKVEVNVGAPQVSFRETFNKESEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQAGLENAMASGPLAGYPMIDIKATLFDGSFHEVDSSEMAFKIAASLALKEAGKVCSPVLLEPIMAIEVTVPEQYFGDTMGDISSRRGLIEGTEQRDNVQVIKAKVPLKEMFGYATDLRSFSQGRGNYVMQFSHYAETPKSVVNEIIATKK", "aa_hexdigest": "f72eaf62423e569b69770962b63c4b0f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75544", "query_cov": 1.0, "subject_cov": 0.815406976744186, "identity": 0.8859999999999999, "score": 978.0, "evalue": 0.0, "valid": false, "gene": "fusA", "product": "elongation factor G", "uniref50_id": "UniRef50_Q5SHN5", "cog_id": "COG0480", "cog_category": "J", "kegg_orthology_id": "K02355", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006414", "GO:0032790" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHN5" ], "product": "Elongation factor G" }, "genes": [], "id": "GIHJHMOALN_526", "locus": "GCA009939745_00491" }, { "type": "cds", "sequence": "contig_1", "start": 278068, "stop": 278715, "strand": "+", "frame": 1, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "GO:1990904", "KEGG:K02990", "RefSeq:WP_010874585.1", "SO:0001217", "UniParc:UPI00001350BA", "UniRef:UniRef100_P75543", "UniRef:UniRef50_P47336", "UniRef:UniRef90_P75543" ], "nt": "ATGCAATACAACATTATTCTTTTAGTTGATGGTTCGCTCTCGTTAGAGCAAGCTAACCAAGTGAATGAAAAACAACAGCAAACGTTAACCAACGTTGAGGGTTTGCAAACAGAATACCTTGGTTTAAAGGAGTTGGCATACCCAATTAAGAAACAACTCTCAGCGCATTATTACCGATGGAAGTTTAGTGGTGATAACCAATCCACGAAGGACTTTAAGCGTACCGCTAACATTAACAAGCAAGTATTACGTGAACTGATTATTAACTTAGAACGTGAGTACGGCTACTTAGCTTCGATAAATCCAAAAAAGCAACAGCTGGCACTGCAAAAACGCGCTAAGTATGACGAAATCATTGCGCGGGAAAACAACCCCGAAAACCCGGATGTACCAGTAACATCAGGTTTAGCTTCCACCCAACCACGCTTATCACGTACTGAAAAGGCACAAAAACCAAAGGAAGAGCTGTGGGATGTGGTGCAAAAGATGGGAAACTTTGACAGCGTTCAAGCCAACCCATACCGCCCACGTTTCAAACGCTTTAACGCGGAACACGTTAACCAACGGCAAAACCAACAAAACAATAACAATAACCGTTTTGACCGCAACCGCAACCGTCAGCACAACCGCTTTAAGGATAAGCAATAA", "aa": "MQYNIILLVDGSLSLEQANQVNEKQQQTLTNVEGLQTEYLGLKELAYPIKKQLSAHYYRWKFSGDNQSTKDFKRTANINKQVLRELIINLEREYGYLASINPKKQQLALQKRAKYDEIIARENNPENPDVPVTSGLASTQPRLSRTEKAQKPKEELWDVVQKMGNFDSVQANPYRPRFKRFNAEHVNQRQNQQNNNNNRFDRNRNRQHNRFKDKQ", "aa_hexdigest": "873e68ea666bf750c16ec1ee7254aa62", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001350BA", "ncbi_nrp_id": "WP_010874585.1", "uniref100_id": "UniRef100_P75543", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001350BA", "RefSeq:WP_010874585.1", "UniRef:UniRef100_P75543" ] }, "ips": { "uniref100_id": "UniRef100_P75543", "uniref90_id": "UniRef90_P75543" }, "psc": { "uniref90_id": "UniRef90_P75543", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_P47336", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47336" ], "product": "Small ribosomal subunit protein bS6" }, "genes": [ "rpsF" ], "id": "GIHJHMOALN_527", "locus": "GCA009939745_00492" }, { "type": "cds", "sequence": "contig_1", "start": 278715, "stop": 279215, "strand": "+", "frame": 3, "gene": "ssb", "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "COG:COG0629", "COG:L", "GO:0003697", "GO:0006260", "GO:0009295", "RefSeq:WP_010874586.1", "SO:0001217", "UniParc:UPI0000135FAD", "UniRef:UniRef100_P75542", "UniRef:UniRef50_P75542", "UniRef:UniRef90_P75542" ], "nt": "ATGAACCGCGTTTTTTTATTTGGTAAGCTCTCGTTTGATCCTAATAAATTGCAAACCCGTACCAACAATATTGGCGCGTCCTTTTCCTTAGCGTGCATTGATTCGAGCGGTTTTAATGACAGTAAGTCCTATATTAGGATTACTGCTTGGGGTAAAGTTGCTAGCTTTGTTTTAACTTTAAAACCCGGTGACAGTGTTTTTGTGGAGGGGAGATTGTCCACTTACAAGATGAACAACCGCAGTGATGATCCCAATTCGAAGGCCACTTACGCGCTCCAAGTTATCGCTGATAAGGTTTACCGTCCCGATGAGGAAAACAGTTTAGAGCAACCTGTTGATAAGGCTACGGTAATTGATTCACCCTTTTTAGCAGCGAAAACCAATGCTACTGAAAATGAATTAGCCCAAGCATTTCCGATTAGTCTGGATGATGAAGATGATGACATTAACCCCATTTTAAACAACGATTCCCAACTAGAGGAGGAGAGTGATGATGAATAA", "aa": "MNRVFLFGKLSFDPNKLQTRTNNIGASFSLACIDSSGFNDSKSYIRITAWGKVASFVLTLKPGDSVFVEGRLSTYKMNNRSDDPNSKATYALQVIADKVYRPDEENSLEQPVDKATVIDSPFLAAKTNATENELAQAFPISLDDEDDDINPILNNDSQLEEESDDE", "aa_hexdigest": "b6ac7ad7b813f9741825c5f23f1a44b2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135FAD", "ncbi_nrp_id": "WP_010874586.1", "uniref100_id": "UniRef100_P75542", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135FAD", "RefSeq:WP_010874586.1", "UniRef:UniRef100_P75542" ] }, "ips": { "uniref100_id": "UniRef100_P75542", "uniref90_id": "UniRef90_P75542" }, "psc": { "uniref90_id": "UniRef90_P75542", "gene": "ssb", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_P75542", "cog_id": "COG0629", "cog_category": "L", "go_ids": [ "GO:0009295", "GO:0003697", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_P75542", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75542" ], "product": "Single-stranded DNA-binding protein" }, "genes": [ "ssb" ], "id": "GIHJHMOALN_528", "locus": "GCA009939745_00493" }, { "type": "cds", "sequence": "contig_1", "start": 279205, "stop": 279519, "strand": "+", "frame": 1, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181", "KEGG:K02963", "RefSeq:WP_010874587.1", "SO:0001217", "UniParc:UPI0000134DA9", "UniRef:UniRef100_P75541", "UniRef:UniRef50_P47338", "UniRef:UniRef90_P75541" ], "nt": "ATGATGAATAACGAACACGATAATTTCCAAAAAGAAGTTGAAACTACAACTGAAACAACCTTCAACAGAGAAGAAGGCAAAAGAATGGTACGTCCTTTATTTAAGCGTTCTAAAAAGTACTGCCGTTTTTGTGCCATTGGTCAACTTAGAATTGACTTAATTGATGATTTAGAAGCGTTAAAGCGTTTCTTGAGTCCCTACGCTAAGATTAACCCACGCCGGATTACTGGTAACTGCCAAATGCATCAGCGCCATGTAGCCAAGGCCTTAAAGCGTGCACGTTACTTAGCCTTAGTTCCGTTTGTAAAAGATTAG", "aa": "MMNNEHDNFQKEVETTTETTFNREEGKRMVRPLFKRSKKYCRFCAIGQLRIDLIDDLEALKRFLSPYAKINPRRITGNCQMHQRHVAKALKRARYLALVPFVKD", "aa_hexdigest": "13d5ab5bae3dc0a1963c684bbcdb0aa6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134DA9", "ncbi_nrp_id": "WP_010874587.1", "uniref100_id": "UniRef100_P75541", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DA9", "RefSeq:WP_010874587.1", "UniRef:UniRef100_P75541" ] }, "ips": { "uniref100_id": "UniRef100_P75541", "uniref90_id": "UniRef90_P75541" }, "psc": { "uniref90_id": "UniRef90_P75541", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_P47338", "cog_id": "COG0238", "cog_category": "J", "kegg_orthology_id": "K02963", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47338" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "GIHJHMOALN_529", "locus": "GCA009939745_00494" }, { "type": "cds", "sequence": "contig_1", "start": 279522, "stop": 279971, "strand": "+", "frame": 3, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02939", "RefSeq:WP_010874588.1", "SO:0001217", "UniParc:UPI00001341F3", "UniRef:UniRef100_P75540", "UniRef:UniRef50_P75540", "UniRef:UniRef90_P75540" ], "nt": "ATGAAGGTAATTTTGAAGCAAGATGTTAGCAACCTAGGCAAACGCTTTGATGTGGTTGATGTTAAAGATGGCTATGCAATCCACTTTTTGTTTCCTAAAAAACTGGCTGCACCTTTAACGAAAAAGAGTTTACAAGACCGTGATTTGTTCTTAAAGAAACAACAAGAACACTATGAGATTAACAAGGCGTTAAGCCACAAGTTAAAGGAAGTAATTGAACAAACAGAACTCCACTTTAGCTTAAAGGAGCACAACGGTCGGCCATATGGCTCGATTATTACCAAGCAAATTATTAACCAAGCCCACACTAAAGGGATGGCGCTGCAAAAGTTTATGTTTAAAGACAACGTGCGCTTAGGGTTTGGGGATCACGAGATTACCTTACACATCTTTGAAGATACAACCGCAGTTTTAAAAGTTAAGGTAACCCCGGATAATGGTGTCAAATAA", "aa": "MKVILKQDVSNLGKRFDVVDVKDGYAIHFLFPKKLAAPLTKKSLQDRDLFLKKQQEHYEINKALSHKLKEVIEQTELHFSLKEHNGRPYGSIITKQIINQAHTKGMALQKFMFKDNVRLGFGDHEITLHIFEDTTAVLKVKVTPDNGVK", "aa_hexdigest": "d5dbdcbeda3b0120f27d9dcdc51fef08", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001341F3", "ncbi_nrp_id": "WP_010874588.1", "uniref100_id": "UniRef100_P75540", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341F3", "RefSeq:WP_010874588.1", "UniRef:UniRef100_P75540" ] }, "ips": { "uniref100_id": "UniRef100_P75540", "uniref90_id": "UniRef90_P75540" }, "psc": { "uniref90_id": "UniRef90_P75540", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_P75540", "cog_id": "COG0359", "cog_category": "J", "kegg_orthology_id": "K02939", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P75540", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75540" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "GIHJHMOALN_530", "locus": "GCA009939745_00495" }, { "type": "cds", "sequence": "contig_1", "start": 279958, "stop": 280593, "strand": "+", "frame": 1, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P75539" ], "nt": "ATGGTGTCAAATAAGCTTGAACAGACCTTTAAGTTTGCCAACGACAAGAACATTGAACGTGCTGAGCGTGTTTTAATGCAAGCTTTAGTACAAGATGCCGTTGGTGTGGACTTAATCTTAACCAAGCTTGAACCGAAGGACTTCTTTGGTTTTCCGTTTAACTTTATCTTTCAAACAGCACAGGAAAACTATTCTGAGGGCAATAAGTTGTTTGGTTCGGCGCTTTTAGAGGCCGTTAAGTTTAAGCTGGATACCGATTCCAAAACACAAAGGGAAATTGAAGGTTTATTTGAGGACGTTTTACTCAAAGGTCTTCCCTTTTTGGAAAGGGACATCAAGGTCTTTGTTGATGTTGTCAAAAAAGCTTCGATTTTTCGCCAACTTAAACAGTTCGCCAAAAAGGTTGAAAAGGAAGAGTTTAAGGTCAAAGATGACCGTTTTGAGGGCTATTTACAAGCAATTCAAAACGACTTTACCAAGATTATTCACAGCGCCTTTGTTTCTATTCCCGGTTTGAGTTATGACGAAATTGGTCGTGAAGAGGAAGAGTTAATTCGCAAAGTGTACCGTGGTGAATTAACTGTAAAAGGGCTTAAAAGTGGTTTTTACCCTTTAGATCAATTAACCTCTGGTTGA", "aa": "MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIGREEEELIRKVYRGELTVKGLKSGFYPLDQLTSG", "aa_hexdigest": "f06d5a9e348bb88eb54d786ec7ffaa2e", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "3-prime", "pseudogene": { "start": 279958, "stop": 280896, "observations": { "mutation": [ 280591 ], "stop": [ 280591 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75539", "query_cov": 1.0, "subject_cov": 0.44608879492600423, "identity": 0.991, "score": 400.0, "evalue": 4.83e-137, "gene_start": 1, "gene_end": 211, "reference_start": 1, "reference_end": 211, "reference_sequence": "MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIVREEEELIPKVYRGELTVKGLKSGFYPLDQLTSGWKEGELIVVAARPGRGKTALLINFLQGAVNDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS" }, "paralog": false, "identity": 0.9903846153846154, "score": 597.0, "evalue": 1.65e-216, "description": "Internal stop codon at 280591. Nonsense mutation around 280591.", "psc": { "uniref90_id": "UniRef90_P75539", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75539", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "GIHJHMOALN_531", "locus": "GCA009939745_00496" }, { "type": "cds", "sequence": "contig_1", "start": 280612, "stop": 281379, "strand": "+", "frame": 1, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P75539" ], "nt": "GTGGTAGCAGCGCGTCCAGGTCGCGGGAAAACAGCGCTTCTAATTAACTTTTTGCAGGGCGCGGTTAATGACAGCGAAAAGTTTGATAAAAACAAGGATGTGTTGCTGTTTTTTAGCTTAGAAATGCGTAACCGTGAAATTTATCAGCGCCATTTAATGCTAGAAAGCCAGGTAAATTACACACTCACGAGTCGTCAAAGGCTCGCTAATGTGTATGATGATTTAATTAAAGCATCAGAAGTATTGCGCGATCTTCCTATTAAAATTTTTGATTACAGCACGATTACGTTGGACGAAATTCGTGCTCAAATCACGGAAGTTTCAAAAACCAATAACGTTAAGTTGGTCGTAATTGACTATCTCCAACTAGTTAATGCATTTAAAAACAGCTATAACATTTCGCGTCAACAAGAGGTCACCATGATTTCCAAATCACTTAAAAGCTTTGCCAAGGAGTTTAACGTACCAATTATTGCCGCTGCACAGCTTTCTCGAAGAATAGAAGAACGCAAAGATTCGCGTCCCATTTTGTCGGATTTACGTGAATCGGGTTCGATTGAACAGGATGCTGACATGGTTTTATTCATCCACCGCGTCAAGGATGAGGACAGTGAAAATCAAGAAGCAATTGGTCACAACAATATTTTTCAAGTGGAGTTAATCTTGGAAAAAAACCGTAGTGGTCCGACTGGTAAAGTACAGTTTGACTTTCAAAGTGACGTTTCTACCTTTCGGGTTCGGGAATACAATCCTGATGGTTACAGTTAG", "aa": "MVAARPGRGKTALLINFLQGAVNDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS", "aa_hexdigest": "ecb9c031c3a78514b207c1530bb8a05d", "start_type": "GTG", "rbs_motif": "AAAA", "genes": [ "dnaB" ], "truncated": "5-prime", "pseudogene": { "start": 280312, "stop": 281379, "observations": { "mutation": [ 280591 ], "stop": [ 280591 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75539", "query_cov": 1.0, "subject_cov": 0.5391120507399577, "identity": 0.996, "score": 485.0, "evalue": 1.61e-169, "gene_start": 1, "gene_end": 255, "reference_start": 219, "reference_end": 473, "reference_sequence": "MVSNKLEQTFKFANDKNIERAERVLMQALVQDAVGVDLILTKLEPKDFFGFPFNFIFQTAQENYSEGNKLFGSALLEAVKFKLDTDSKTQREIEGLFEDVLLKGLPFLERDIKVFVDVVKKASIFRQLKQFAKKVEKEEFKVKDDRFEGYLQAIQNDFTKIIHSAFVSIPGLSYDEIVREEEELIPKVYRGELTVKGLKSGFYPLDQLTSGWKEGELIVVAARPGRGKTALLINFLQGAVNDSEKFDKNKDVLLFFSLEMRNREIYQRHLMLESQVNYTLTSRQRLANVYDDLIKASEVLRDLPIKIFDYSTITLDEIRAQITEVSKTNNVKLVVIDYLQLVNAFKNSYNISRQQEVTMISKSLKSFAKEFNVPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRVKDEDSENQEAIGHNNIFQVELILEKNRSGPTGKVQFDFQSDVSTFRVREYNPDGYS" }, "paralog": false, "identity": 0.9915492957746479, "score": 677.0, "evalue": 2.11e-247, "description": "Internal stop codon at 280591. Nonsense mutation around 280591.", "psc": { "uniref90_id": "UniRef90_P75539", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75539", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "GIHJHMOALN_532", "locus": "GCA009939745_00497" }, { "type": "cds", "sequence": "contig_1", "start": 281383, "stop": 281793, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTACAAAACAGTTAAATCGATCCCGCTTTTTTTGTTAGGCTCGATTGTGTTCACTGCTTGTTCAACACCACAATCAACCTTTCATTTACCTGTGCAAACGACCGTTAGCGCAATCAAAAAAGATATTAGTGGGAAAACAGCTACTGCTGTTAAGGCTGCTAGTAGTTCATCTAGTACTACTACATCTAACGATGACAACAACCAAAAGGGTTACTTCTTAGAAACTAATCGTTCCACCGGTACTTACGACCCTAACAATTCCACCCGGTTAATTAAATTAGGTGAAAGTGGTGATTTTCATGCAGCTGACCAGAATAAACCGGAAGAAGCCTTGTTTGAACGACTTTACGGCGGCATTGCTTCCTTATTAAATTTCCGCATTATTAAACCTGCTTTAACTTATTGA", "aa": "MKYKTVKSIPLFLLGSIVFTACSTPQSTFHLPVQTTVSAIKKDISGKTATAVKAASSSSSTTTSNDDNNQKGYFLETNRSTGTYDPNNSTRLIKLGESGDFHAADQNKPEEALFERLYGGIASLLNFRIIKPALTY", "aa_hexdigest": "3c9bf81ed1388f87208e723ee440fe3a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14759.450199999985, "isoelectric_point": 9.239302635192868 }, "id": "GIHJHMOALN_533", "locus": "GCA009939745_00498" }, { "type": "cds", "sequence": "contig_1", "start": 281887, "stop": 282513, "strand": "+", "frame": 1, "gene": null, "product": "putative lipo MG095-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75538", "UniRef:UniRef90_P75538" ], "nt": "TTGGCTAATAACTTAATTGTCGCTGATGATGGCAACAACAACTTTTGGTTTGGTTTAAAGTCACTTAGCTTTAATGCAGCTAAGTTGACTGACAGCGCCCAAACACAAATGGCGCAAAAAACCACTCAAGCAGTTACCTTGAAAAGTCAAGCGCAAATGTCAAGTACAAATACCAAAAACACCAACAAAAAGATTGATTTGCGCGATAAGATTACTTTATCGAGTACGATGAATACTCAAGGAAGTGGTGATAACAAAAACCCATCTAGTGGTCTTATTCAAAAGTTAGTGAGTGTCGAAAATATTGAAGCTGAATTTAGTTTTGTTAAAACTGGCTTCAACGGTAATGAAATTAAATTTGGCGATTTTGTAACTGAGAATTCACCCACAACCACTCAACTTAAGCAAGTTTGGAAAAAGAAGTGGGGCACGGAATTGAAAAAGACAAACTATAAGCTTCAATTGAACAACTTTAGTCTATTGCTAACTTACACACCAGAAGTTAATAAAGTTGAAAAAGGTAACAATGGTGATTCCAATAAAGGCACGATCGCTACGCCAAACGGTTTTAGCTTCTTGTACCCAGCTAATTTAAATGAGACACCAAGCAGTAGCAGTAGTTATTGA", "aa": "MANNLIVADDGNNNFWFGLKSLSFNAAKLTDSAQTQMAQKTTQAVTLKSQAQMSSTNTKNTNKKIDLRDKITLSSTMNTQGSGDNKNPSSGLIQKLVSVENIEAEFSFVKTGFNGNEIKFGDFVTENSPTTTQLKQVWKKKWGTELKKTNYKLQLNNFSLLLTYTPEVNKVEKGNNGDSNKGTIATPNGFSFLYPANLNETPSSSSSY", "aa_hexdigest": "997ab548c6713105ff3b405ba37f5068", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 281590, "stop": 282747, "observations": { "mutation": [ 281791, 282511 ], "stop": [ 281791, 282511 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75538", "query_cov": 1.0, "subject_cov": 0.4581497797356828, "identity": 0.986, "score": 393.0, "evalue": 2.49e-134, "gene_start": 1, "gene_end": 208, "reference_start": 169, "reference_end": 376, "reference_sequence": "MKYKTVKSIPLFLLGSIVFTACSTPQSTFHLPVQTTVSAIKKDISGKTATAVKAASSSSSTTTSNDDNNQKGYFLETNRSTGTYDPNNSTRLIKLGESGDFHAADQNKPEEALFERLYGGIASLLNFRIIKPALTYWNTVTPSLKAIGKSSNLITFSQDIDETELQRALANNLIVADDGNNNFWFGLKSLSFNSAKLTDNAQTQMAQKTTQAVTLKSQAQMSSTNTKNTNKKIDLRDKITLSSTMNTQGSGDNKNPSSGLIQKLVSVENIEAEFSFVKTGFNGNEIKFGDFVTENSPTTTQLKQVWKKKWGTELKKTNYKLQLNNFSLLLTYTPEVNKVEKGNNGDSNKGTIATPNGFSFLYPANLNETPSSSSSYWTNVTDLTKAATDTENTNLLNDLQKSQEQVNQFVAAITQNHLDVSEAALTKKQFGSLSISDFFKAIFKENGKDTKAKS" }, "paralog": false, "identity": 0.9896103896103896, "score": 749.0, "evalue": 5.56e-277, "description": "Internal stop codon at 281791, 282511. Nonsense mutation around 281791, 282511.", "psc": { "uniref90_id": "UniRef90_P75538", "product": "Uncharacterized lipoprotein MG095 homolog", "uniref50_id": "UniRef50_P75538", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75538", "UniRef:UniRef50_P75538", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75538", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75538" ], "product": "Uncharacterized lipoprotein MG095 homolog" } }, "id": "GIHJHMOALN_534", "locus": "GCA009939745_00499" }, { "type": "cds", "sequence": "contig_1", "start": 282577, "stop": 282747, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAAGAGTCAGGAACAAGTCAATCAATTTGTTGCTGCTATTACCCAAAATCACCTCGATGTTTCCGAAGCTGCTTTAACTAAAAAACAGTTTGGTAGCCTTAGCATTAGTGATTTCTTTAAAGCCATCTTTAAGGAAAATGGCAAAGACACTAAAGCTAAAAGTTAA", "aa": "MQKSQEQVNQFVAAITQNHLDVSEAALTKKQFGSLSISDFFKAIFKENGKDTKAKS", "aa_hexdigest": "2b4ec1a1cb8e74bb232977eaeff3cd23", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6233.969299999999, "isoelectric_point": 9.158652687072756 }, "id": "GIHJHMOALN_535", "locus": "GCA009939745_00500" }, { "type": "cds", "sequence": "contig_1", "start": 283175, "stop": 284218, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_234", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75537", "UniRef:UniRef90_P75537" ], "nt": "TTGACTGGTGTACTTGAAACAATTCCCCAACTGCAAAAGGAAATAAAAGCTGCTAATGACATTTTCCCTGTCAATGAACAAAGTCAAATTCCCCGTGTAGAAGCACGCTTATTTGACTTTTTGGGCAGTGGCTTTTTCCCGGTATTGAACCCGAAAGGTTTAGGGTTAGCGGACAACGTTGCTAGTTTGTTTGAACAGTTTCAGCTAGCATCAATAAACTTAAGTGGTTTTGAAGTCAAACTGATTAAAACGCATGACATTGTCATTAAGAGTAAGGTACGTTACAGCTTTGAAGTGCAAATGCAGTTCCATGCTGTTTACCAAAATAACGACACTAAAGCTAATTTAGATTTTGCGCTCAATATTAGTACGATTAACTTCGCTACCTTACAAGAATTACAAAACAGTTTTGATCTTCAAGGTAGTGATTTAACGGCTGGGTTGTTTTACAAATACAGCGTTAATAAGTTAACTAGTGGTACTAACGATTTAACCACAATTGCTAAAACCGCTTTAGGTGAAAACATTATCCAAAAGCAAGTGAGCCTAACACAATCAATTATTAAGCCACGCCTAGAAGCTGCAAAAACTCAGTACAAACAGGATATTATTGCACCATTTGCAAAGGAACGTCAAGCTGCTTTGGCACAACATTTAAAAGAAATAGAAGAGGCCAAACAACGTGCCGAGCAATTATTAAAAGAACAGCAAGAAGCGGAAAAACGGCGCCAAGAAGAAGTTAAGAATGTCGCAGAAACACAACAGTTTAATGATAGCTTAACTTCAGCACAAAAATTTAAAGAGTACTGGTTAAAACAAGGTAAGGATGTTACCAAAAAGGTTGAACTCATTCAGGCGCTGAAATCATCTTTCTTCCGCAACCAAAACCGTACCTTTAACTTTTTAATTGCCGGTTTTCGAACTGCCATTGATTGGTACTACAATCAAGAGAAAAATAATACCACTGCTAAAAATAACGCTTTTGGTAAGAATGGCATTCAGTTCCCTGTGGCTGGCTTTCAGGGAATTTACATGTCACAGTGA", "aa": "MTGVLETIPQLQKEIKAANDIFPVNEQSQIPRVEARLFDFLGSGFFPVLNPKGLGLADNVASLFEQFQLASINLSGFEVKLIKTHDIVIKSKVRYSFEVQMQFHAVYQNNDTKANLDFALNISTINFATLQELQNSFDLQGSDLTAGLFYKYSVNKLTSGTNDLTTIAKTALGENIIQKQVSLTQSIIKPRLEAAKTQYKQDIIAPFAKERQAALAQHLKEIEEAKQRAEQLLKEQQEAEKRRQEEVKNVAETQQFNDSLTSAQKFKEYWLKQGKDVTKKVELIQALKSSFFRNQNRTFNFLIAGFRTAIDWYYNQEKNNTTAKNNAFGKNGIQFPVAGFQGIYMSQ", "aa_hexdigest": "9cf2a8e6f1af61ca881d13d8d0705a27", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 282875, "stop": 284521, "observations": { "mutation": [ 283064, 283163, 284216, 284408, 284450 ], "stop": [ 283064, 283163, 284216, 284408, 284450 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75537", "query_cov": 1.0, "subject_cov": 0.5481832543443917, "identity": 0.997, "score": 630.0, "evalue": 8.41e-223, "gene_start": 1, "gene_end": 347, "reference_start": 117, "reference_end": 463, "reference_sequence": "MKFPKKRWQKWAFLTGSFTVLPTLIAFFSILPHSSVLEPSHLRFNSTSQRYQSDNSHLLNLHGEAFSDADLNAVAEHTDWDANFALFQQKFKTKPITVNQLGLVDVFNLLSGWKQSLTGVLETIPQLQKEIKAANDIFPVNEQSQIPRVEARLFDFLGSGFFPVLNPKGLGLADNVASLFEQFQLASINLSGFEVKLIKTHDIVIKSKVRYSFEVQMQFHAVYQNNDTKANLDFALNISTINFATLQELQNSFDLQGSDLTAGLFYKYSVNKLTSGTNDLTTIAKTALGENIIQKQVSLTQSIIKPRLEAAKTQYKQDIIAPFAKERQAALAQHLKEIEEAKQRAEQLLKEQQEAEKRRQEEVKNVAETQQFNDSLTSAQKFKEYWLKQGKDVTKKVELIQALKSSFFRNQNRTFNFLIAGFRTAIDWYYNQEKNNTTAKNNAFGKNGIQFPVAGFQGIYMSQWLRDELSGKTDIKLNLKSLSVQNENKNSSINWNKQKRIEIKQVKPFNYSFEINLKYTGSYNVSLWYLIGAAIGGIPTSWSGTMDMKFIVDGDLDSGIVTKQDYPGSKFEFTEDKLWFTLHVKQQIKVKEQGFMNLLKGQSLDNLDLRTGTTKPPVVDLASYLHFVILTAK" }, "paralog": false, "identity": 0.9908759124087592, "score": 1021.0, "evalue": 0.0, "description": "Internal stop codon at 283064, 283163, 284216, 284408, 284450. Nonsense mutation around 283064, 283163, 284216, 284408, 284450.", "psc": { "uniref90_id": "UniRef90_P75537", "product": "Uncharacterized protein MPN_234", "uniref50_id": "UniRef50_P75537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75537", "UniRef:UniRef50_P75537" ] }, "pscc": { "uniref50_id": "UniRef50_P75537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75537" ], "product": "Uncharacterized protein MPN_234" } }, "id": "GIHJHMOALN_536", "locus": "GCA009939745_00501" }, { "type": "cds", "sequence": "contig_1", "start": 284219, "stop": 284410, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "TTGCGTGACGAGCTCTCTGGTAAGACCGACATTAAGCTCAACTTAAAAAGTTTAAGTGTTCAGAATGAAAATAAAAATTCTTCCATTAATTGGAACAAGCAAAAAAGAATTGAGATTAAACAAGTAAAACCATTTAACTATTCTTTTGAAATTAACTTGAAGTACACTGGTAGCTATAATGTCAGTCTTTGA", "aa": "MRDELSGKTDIKLNLKSLSVQNENKNSSINWNKQKRIEIKQVKPFNYSFEINLKYTGSYNVSL", "aa_hexdigest": "b220fc0c1ec9eeda9cb2e3f0537a031c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 70, "aa_cov": 0.9841269841269841, "hmm_cov": 1.0, "evalue": 6.96358993060381e-19, "score": 63.76200866699219, "start": 1, "stop": 62 } ], "seq_stats": { "molecular_weight": 7395.322900000001, "isoelectric_point": 9.697672843933105 }, "id": "GIHJHMOALN_537", "locus": "GCA009939745_00502" }, { "type": "cds", "sequence": "contig_1", "start": 284462, "stop": 284728, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGGATATGAAGTTTATCGTTGATGGTGATTTGGATAGCGGTATTGTTACCAAGCAAGATTATCCCGGTTCTAAGTTTGAATTTACCGAAGACAAACTGTGGTTTACCCTGCACGTTAAACAACAAATTAAAGTTAAAGAGCAGGGCTTTATGAACTTGCTTAAGGGTCAAAGTTTGGACAACTTGGATCTACGCACTGGTACAACTAAACCGCCAGTAGTTGATTTAGCGAGCTACCTCCACTTTGTTATTTTGACAGCTAAGTAG", "aa": "MDMKFIVDGDLDSGIVTKQDYPGSKFEFTEDKLWFTLHVKQQIKVKEQGFMNLLKGQSLDNLDLRTGTTKPPVVDLASYLHFVILTAK", "aa_hexdigest": "26088d073aaa33af3526bbdd31353688", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 48, "aa_cov": 0.5340909090909091, "hmm_cov": 1.0, "evalue": 5.0729470287032136e-18, "score": 60.96890640258789, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 10034.49619999999, "isoelectric_point": 6.040245628356933 }, "id": "GIHJHMOALN_538", "locus": "GCA009939745_00503" }, { "type": "cds", "sequence": "contig_1", "start": 284753, "stop": 285094, "strand": "+", "frame": 2, "gene": "ung", "product": "uracil-DNA glycosylase", "db_xrefs": [ "COG:COG0692", "COG:L", "EC:3.2.2.27", "GO:0004844", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0097510", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_P75536", "UniRef:UniRef90_P75536" ], "nt": "ATGGAACAACTTTTAGACCAGTTTTTAAGTGGTGTTTTAAGCGAATGGAAGGCTTTTATTTTGCAAGAAGCCAAACAGACCTATTTTCAAGAATTGTTAGCTAAACTAAAAAATGTCGAGCAAGAGCTAACTCCCAAAGCCTCTCAAGTCTTTCGTCCCTTTAGCTTTTTTGCACCAAATAACACTAAACTAATTATTTACGGTCAGGATCCATATCCTAATCCACAACACGCTTGTGGTCTTTCGTTTGCCTCTAACGCCCCTAAACTACCACAAAGCTTAAAGCGGATGATTCTGCGCTTGCAGCAAGAGTATCCGGAACTAGCAGGTCAAAATCACTGA", "aa": "MEQLLDQFLSGVLSEWKAFILQEAKQTYFQELLAKLKNVEQELTPKASQVFRPFSFFAPNNTKLIIYGQDPYPNPQHACGLSFASNAPKLPQSLKRMILRLQQEYPELAGQNH", "aa_hexdigest": "49a9ea9bbb4c4529cd72f5f243a6f61a", "start_type": "ATG", "rbs_motif": null, "genes": [ "ung" ], "truncated": "3-prime", "pseudogene": { "start": 284753, "stop": 285397, "observations": { "mutation": [ 285092, 285116, 285197 ], "stop": [ 285092, 285116, 285197 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75536", "query_cov": 1.0, "subject_cov": 0.4708333333333333, "identity": 1.0, "score": 226.0, "evalue": 1.96e-73, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MEQLLDQFLSGVLSEWKAFILQEAKQTYFQELLAKLKNVEQELTPKASQVFRPFSFFAPNNTKLIIYGQDPYPNPQHACGLSFASNAPKLPQSLKRMILRLQQEYPELAGQNHWTKQLLEGWAQQGILLLNGVFTTNAFQTNAHRNWGWEQFNCHLLDFLLSQKLYVLLVFLGKQTENFVLKKIGGASQFASLSYPHPSPLTGRKFFDHPDALFKQINQWLKHHNHTPIDWTNGQVQYQF" }, "paralog": false, "identity": 0.985981308411215, "score": 425.0, "evalue": 4.22e-154, "description": "Internal stop codon at 285092, 285116, 285197. Nonsense mutation around 285092, 285116, 285197.", "psc": { "uniref90_id": "UniRef90_P75536", "gene": "ung", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_P75536", "cog_id": "COG0692", "cog_category": "L", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0097510" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75536", "EC:3.2.2.27", "UniRef:UniRef50_P75536", "COG:COG0692", "COG:L", "KEGG:K03648", "GO:0004844", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0097510" ] }, "pscc": { "uniref50_id": "UniRef50_P75536", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75536" ], "product": "Uracil-DNA glycosylase" } }, "id": "GIHJHMOALN_539", "locus": "GCA009939745_00504" }, { "type": "cds", "sequence": "contig_1", "start": 285673, "stop": 286638, "strand": "+", "frame": 1, "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "db_xrefs": [ "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681", "SO:0001217", "UniRef:UniRef50_P47344", "UniRef:UniRef90_P75535" ], "nt": "ATGGATAAATTAATAAATAAACCTCAACCATTAACTTCTGATATTTCCACTTCTGGATTTATTTACTTTGCGGTTGTTTTTGTAGCCATTATGGGCTACCTTTTATTCAAAAATCTACTGTTTTTATTTTTCTTTAAACGCTATCCAAAAAACACTACCAAAATTGGGGTAGGTAACATTACCACGATTGCCATGATCATTGCTGTGGCGGTGTCGATTGTTTTAGTGTTAATGGCACTAGCAGGCGGTTTAGCCGCTGCTTTGTTCCGTGGTTATCCTGGTTTTCGGGTCACCTTGGAGTTAATCCTTGTAAAGATTTCGGGACTGTTGTTTGGTCCAATTGTCGGCATTTTTTCCGCGGCCACAATTGACTTTTTAACGGTCATCTTTTCGGGTGGGGTTTTTAACGTTGGTTATGTTTTAGGTGCCATCTTAACCGGGATGATTGCTGGCATACTGCGTGAAGTTTTAATTTCAACAGCACTTTTACACAACCGTAATTTATCGGATTTTGCCTACCTAGTGCTCTCGATTGGCATGGTCATTGCCGCCTTTTTAATTACCCAGTTCTTTGTGCTTGGGATAAGTAATAACCTTAAGGAGATTAAGGGTGATGAAGAGTTTCGCTTGAAATTTAACGCACCACCAATTGTCTTTGAGCTTTCGTTAACCCAGTACGCCAACATTCTGCTCTACTTTACCATTGCCATAGTTATTGCTATGCTGGTGTTGTACATTGTTTGGTTAGTCAAACAGCGTCACTTAAGTTTTGAGCACTCTCGTTTCTTTTACCGCAGTTACAAACATGCTAATCACCAGTTTACCCTGTTTGTACTAACTAAGGAAAACTGGTTCTATTTAATCTTGAATGTAATCACCTTAGCTTCCACTAGTTTGTTGATGATCAACATTGCCTTCATTCCGATCTTTGACACGCAAACAACTGGTCAAACATACGAGTTTTGA", "aa": "MDKLINKPQPLTSDISTSGFIYFAVVFVAIMGYLLFKNLLFLFFFKRYPKNTTKIGVGNITTIAMIIAVAVSIVLVLMALAGGLAAALFRGYPGFRVTLELILVKISGLLFGPIVGIFSAATIDFLTVIFSGGVFNVGYVLGAILTGMIAGILREVLISTALLHNRNLSDFAYLVLSIGMVIAAFLITQFFVLGISNNLKEIKGDEEFRLKFNAPPIVFELSLTQYANILLYFTIAIVIAMLVLYIVWLVKQRHLSFEHSRFFYRSYKHANHQFTLFVLTKENWFYLILNVITLASTSLLMINIAFIPIFDTQTTGQTYEF", "aa_hexdigest": "75011c6b5b8046edf9c4bdcc2e2ca262", "start_type": "ATG", "rbs_motif": null, "genes": [ "gatC" ], "truncated": "3-prime", "pseudogene": { "start": 285673, "stop": 286941, "observations": { "mutation": [ 286636 ], "stop": [ 286636 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75535", "query_cov": 1.0, "subject_cov": 0.6701461377870563, "identity": 0.9940000000000001, "score": 576.0, "evalue": 3.7e-204, "gene_start": 1, "gene_end": 321, "reference_start": 1, "reference_end": 321, "reference_sequence": "MDKLINKPQPLTSDISTSGFIYFAVVFVAIMGYLLFKNLLFLFFFKRYPKNTPKIGVGNITTIAMIIAVAVSIVLVLMALAGGLAAALFRGYPGFRVTLELILVKISGLLFGPIVGIFSAATIDFLTVIFSGGVFNVGYVLGAILTGMIAGILREVLISTALLHNRNLSDFAYLVLSIGMVIAAFLITQFFVLGISNNLKEIKGDEEFRLKFNAPSIVFELSLTQYANILLYFTIAIVIAMLVLYIVWLVKQRHLSFEHSRFFYRSYKHANHQFTLFVLTKENWFYLILNVITLASTSLLMINIAFIPIFDTQTTGQTYEFWLLARLLFAPVIFLLDIIVIYPILLLLTPLMLKGFKTAVSKNQRKTLKQSFTDLQSVVLPIINKRKHQQLRQEELKRLARATHFDLTEGEMEKLLVEFKTITQSFDRVMNIDTTSVEPMYAPFNTSPTPLRKDKVIVEKHPEKLLANCKEMSVGFVKV" }, "paralog": false, "identity": 0.9928909952606635, "score": 800.0, "evalue": 9.78e-295, "description": "Internal stop codon at 286636. Nonsense mutation around 286636.", "psc": { "uniref90_id": "UniRef90_P75535", "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "uniref50_id": "UniRef50_P47344", "cog_id": "COG0721", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75535", "UniRef:UniRef50_P47344", "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P47344", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47344" ], "product": "Uncharacterized protein MG098" } }, "id": "GIHJHMOALN_540", "locus": "GCA009939745_00505" }, { "type": "cds", "sequence": "contig_1", "start": 286675, "stop": 287112, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGGACATTATTGTGATCTATCCAATTCTGCTGTTATTAACCCCGCTAATGCTCAAAGGTTTTAAAACAGCAGTTTCTAAAAACCAACGCAAAACACTAAAACAAAGCTTTACTGATTTGCAAAGTGTAGTGTTGCCCATTATTAACAAACGTAAACACCAGCAGTTGCGCCAAGAAGAGTTAAAACGCTTAGCACGAGCCACTCACTTTGATTTGACAGAAGGCGAAATGGAAAAGCTCTTGGTCGAATTTAAAACTATTACCCAGAGCTTTGACCGGGTGATGAACATTGATACCACCAGTGTGGAACCAATGTACGCACCTTTCAATACATCACCAACGCCACTGCGTAAAGATAAGGTAATAGTGGAAAAGCATCCCGAAAAATTATTAGCCAACTGTAAGGAAATGAGTGTTGGCTTTGTCAAAGTATAG", "aa": "MLDIIVIYPILLLLTPLMLKGFKTAVSKNQRKTLKQSFTDLQSVVLPIINKRKHQQLRQEELKRLARATHFDLTEGEMEKLLVEFKTITQSFDRVMNIDTTSVEPMYAPFNTSPTPLRKDKVIVEKHPEKLLANCKEMSVGFVKV", "aa_hexdigest": "b81d3988cf6cbfa4502208105427e717", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16753.69129999999, "isoelectric_point": 9.605740928649901 }, "id": "GIHJHMOALN_541", "locus": "GCA009939745_00506" }, { "type": "cds", "sequence": "contig_1", "start": 287367, "stop": 287516, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "TTGGAAGACTATATCCCGCCGTTTTCAGCAACTGTGTTTGAATTGCTAAACAACAGTGGCGCTTTATTAGTTGGTAAAGCGAACCTCGACGAGTTTGGTTTAGGCGGTACGGGGCTCCATTCGGGTTTTGGTTTTGTCCACCACCCTTGA", "aa": "MEDYIPPFSATVFELLNNSGALLVGKANLDEFGLGGTGLHSGFGFVHHP", "aa_hexdigest": "1f21a5e9cf2fece19ab7d8f96b820894", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 48, "aa_cov": 0.9795918367346939, "hmm_cov": 1.0, "evalue": 7.272735623224899e-13, "score": 43.77743148803711, "start": 2, "stop": 49 } ], "seq_stats": { "molecular_weight": 5174.751499999999, "isoelectric_point": 4.834747505187988 }, "id": "GIHJHMOALN_542", "locus": "GCA009939745_00507" }, { "type": "cds", "sequence": "contig_1", "start": 287565, "stop": 287924, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "GTGGCGCGCGGCATTGTTCCCTTTTCAATTGGTACGGACACGGGAGATTCGGTACGCCGTCCAGCTAGCATTTGTAACATTGTTGGTTTTAAGCCCACCTATGGTTTGATATCGCGGAACGGAGTGTATCCTTACGCGCCTAGTTTAGACCATGTCGGGATCTTTGCGCGGTATGTGTATGATGTAGCACTTGTTAGTGATGAAATCATTAAACATGACAAAGCTGATTTTTCCGCTCAAAAATCTCCTGATGCTGGCAAGTTTACCAGGTCCTTAAAAGAGTCCTTCAACAAGCAAATCAAGATAGGTTATCTAAAACCGCTAGAGGAATGGTTTGACATTGAACTGAGCAAAAAGTGA", "aa": "MARGIVPFSIGTDTGDSVRRPASICNIVGFKPTYGLISRNGVYPYAPSLDHVGIFARYVYDVALVSDEIIKHDKADFSAQKSPDAGKFTRSLKESFNKQIKIGYLKPLEEWFDIELSKK", "aa_hexdigest": "d40eabf11527c5f792c7ba569a20d339", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 113, "aa_cov": 0.9495798319327731, "hmm_cov": 0.9911504424778761, "evalue": 6.126625652164352e-41, "score": 136.30691528320312, "start": 2, "stop": 114 } ], "seq_stats": { "molecular_weight": 13303.072799999987, "isoelectric_point": 8.989681053161618 }, "id": "GIHJHMOALN_543", "locus": "GCA009939745_00508" }, { "type": "cds", "sequence": "contig_1", "start": 287949, "stop": 288428, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "TTGGAGGGTTGTGAGTTAATTCCGTTTCACTTTCCGCTTGAACTGCTAGAGGTAATTGATCCAGTTTACAAGCTGATTAGTTACAGTGAAGCGGTGAGCTGTTACAGTAATCTCACTGGGATTGTCTTTGGTCAAAAATTGTTTGAACCCAACCAAGCGAGTGACTTTTCCAAAACAATTACTGCCAACCGCGACCGTTTCTTTGGTGAGCAGTTAAAACGCCGCTTTATTATTGGTGCTTTTGGTACTGACAAGAATAACTTTACCAAGTACTTTGAAAAGGCTCAAAAGATCCGCCGGGTAATGGTTGATGCTTACTTAAACCTGTTTAAAGAAGCGGATTTCATTGTGTCACCTAGTGCATCCGGCTTTACTAAAACAATAGCTGCGGTGCAAAAGGGTGAGAGCTTTACTAACCTCGTGGATGACTTTTTACAACTAGCTAACTTTGCTGGTAATCCTTCAATTACTATTCCCTGA", "aa": "MEGCELIPFHFPLELLEVIDPVYKLISYSEAVSCYSNLTGIVFGQKLFEPNQASDFSKTITANRDRFFGEQLKRRFIIGAFGTDKNNFTKYFEKAQKIRRVMVDAYLNLFKEADFIVSPSASGFTKTIAAVQKGESFTNLVDDFLQLANFAGNPSITIP", "aa_hexdigest": "dc2a056738c208e9952c47c45cafb867", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 162, "aa_cov": 0.9874213836477987, "hmm_cov": 0.9382716049382716, "evalue": 8.476151240353718e-27, "score": 89.70026397705078, "start": 3, "stop": 159 } ], "seq_stats": { "molecular_weight": 17931.30759999999, "isoelectric_point": 5.8598955154418935 }, "id": "GIHJHMOALN_544", "locus": "GCA009939745_00509" }, { "type": "cds", "sequence": "contig_1", "start": 288544, "stop": 289977, "strand": "+", "frame": 1, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "COG:COG0064", "COG:J", "EC:6.3.5.-", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681", "KEGG:K02434", "RefSeq:WP_014574916.1", "SO:0001217", "UniParc:UPI000012B157", "UniRef:UniRef100_P75533", "UniRef:UniRef50_P75533", "UniRef:UniRef90_P75533" ], "nt": "ATGATTAACTTTGAAGCAATTATTGGGATTGAGGTACACGTGGTCTTAAACACTGCTACCAAAATGTTTTCCCCAGCGCCCAACCAAGCGCAAAACGCCACACCAAACCAGTTCATAAACGCGATTGATTTGGGCTTGCCTGGTACCATGCCCCAGGTCAATGAGCAGGCGGTACAAAAGGCGTTAATCCTTGCTGACGCGCTCAACATGCAACGGGTACAGCCAGTCTTAGTGTTTGACCGTAAGCACTACTTTTACCAAGATCTACCTAAGGGTTTTCAGATTACCCAGCAAAACTTTCCGATTGCGCAAAACGGTTACGTTGAAATTGTGGAAAACAACAAAGCGCAGCGGATTATCATTGAACGGTTTCACCTAGAGGAAGATACTGCCAAGCAACATTTTGTCGATGGGCAAATTCTGCTCGACTTTAACCGTTGTGGTGCACCGCTAATTGAAGTAGTTACCGCGCCCGTGATTAAGAGTGCTAAGCAGTCTAAGGCATACTTGCAAAAGCTACGTCAAATTTTGATTGTTAACAACATTTCCAACGCTAAGCTCGAAGATGGCTCGATGCGCAGTGACTGTAATGTATCAGTGCGCTTAAAGGGACAAACTGCTTTTGGCACCAAGGTCGAAATTAAGAACATTAACTCACTCAATAATGTGGAAAAGGCAATTGATTTGGAAATAGCACGTCAAGTCAAAGCACTAATTAAAGGCGAACCGGTTCAACAAGTTACCCTAACTTATGACGACAAAACCAATACCAATGTCTTCATGCGTAAAAAGGACAATTCAGTCGATTACCGTTATTTCATTGAACCCAACATTATGAGCAGTAACATTGATGAACTGCTACAAAAACCCAACAAGGCCTTTAACTTAACTGAGTTCTTTACCAAGCTCAAGGAAGCTGGGGTTAATGAGCAGCTAAATCAATTAGTAGTCGATGATCTGACTCTTTTTAATGCTTACACCAAGATTAACAGTGTCATTAACAGTCCCCAAGATACCATTCGCTGGTTGTGCATTGAGTTAATGGGACAACTCAACAAGCTACAAAAACCGCTCAAGGACAAAACGATTGATCACTTAATTATCCTAATCCAAATGGTACAGAAGGGCACCGTGAACCAAAAACAAGCAAAGCAGTTAATTGAGTTAATGTTAGACAATGGGCAAGACCCCCAATCGCTGGCTAAACTACACAACTTAGAACAGATAACTGATGAAAAACAACTAACGCAAATCATCCAACAAATCTTTAAGGAAAACGAGGGTGAAATCCTTAAGAACTTAGATCGAGTTGAACGAATTCAAAAGCTAATTATAGGTCAAGTTATGCAAAGAACCCACAATCGCGCCAATCCCCAGCAAGTTTTTATAATTGTTGAAAAGTTGCTTCATGATTTCAGCGAAAGAGCAAGCTAA", "aa": "MINFEAIIGIEVHVVLNTATKMFSPAPNQAQNATPNQFINAIDLGLPGTMPQVNEQAVQKALILADALNMQRVQPVLVFDRKHYFYQDLPKGFQITQQNFPIAQNGYVEIVENNKAQRIIIERFHLEEDTAKQHFVDGQILLDFNRCGAPLIEVVTAPVIKSAKQSKAYLQKLRQILIVNNISNAKLEDGSMRSDCNVSVRLKGQTAFGTKVEIKNINSLNNVEKAIDLEIARQVKALIKGEPVQQVTLTYDDKTNTNVFMRKKDNSVDYRYFIEPNIMSSNIDELLQKPNKAFNLTEFFTKLKEAGVNEQLNQLVVDDLTLFNAYTKINSVINSPQDTIRWLCIELMGQLNKLQKPLKDKTIDHLIILIQMVQKGTVNQKQAKQLIELMLDNGQDPQSLAKLHNLEQITDEKQLTQIIQQIFKENEGEILKNLDRVERIQKLIIGQVMQRTHNRANPQQVFIIVEKLLHDFSERAS", "aa_hexdigest": "4e03be2185b8f4886ffdaf587fc56391", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B157", "ncbi_nrp_id": "WP_014574916.1", "uniref100_id": "UniRef100_P75533", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B157", "RefSeq:WP_014574916.1", "UniRef:UniRef100_P75533" ] }, "ips": { "uniref100_id": "UniRef100_P75533", "uniref90_id": "UniRef90_P75533" }, "psc": { "uniref90_id": "UniRef90_P75533", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.-" ], "uniref50_id": "UniRef50_P75533", "cog_id": "COG0064", "cog_category": "J", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P75533", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75533" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" }, "genes": [ "gatB" ], "id": "GIHJHMOALN_545", "locus": "GCA009939745_00510" }, { "type": "cds", "sequence": "contig_1", "start": 289952, "stop": 290233, "strand": "+", "frame": 2, "gene": "mngR", "product": "putative HTH-type transcriptional regulator MG101-like protein", "db_xrefs": [ "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700", "SO:0001217", "UniRef:UniRef50_P75532", "UniRef:UniRef90_P75532" ], "nt": "ATGATTTCAGCGAAAGAGCAAGCTAAAAAAGGTCAAATAATTTACCGTTACATTCTCCTTAAAATTCAAAGCTTTAAATGGCCTGCTGGCACCCGCATCTTCTCTGAACGCCAGCTCGAAATTCGTTTCGCTTCATCTAGAAGTCTCATTCGTACTATCCTCAATACCCTCTTAGGTAAAGATCTAATTCGCCACACTAAAGGTAATGCTGGTTACTTTGTCGCTAAGAATGCTGGTTTTAGTTTCTTTCATAAAACCCAGGACAACAAATTGCCCAAGTGA", "aa": "MISAKEQAKKGQIIYRYILLKIQSFKWPAGTRIFSERQLEIRFASSRSLIRTILNTLLGKDLIRHTKGNAGYFVAKNAGFSFFHKTQDNKLPK", "aa_hexdigest": "f903fecc7b0700eb7a22266261b6d67a", "start_type": "ATG", "rbs_motif": null, "genes": [ "mngR" ], "truncated": "3-prime", "pseudogene": { "start": 289952, "stop": 290536, "observations": { "mutation": [ 290231 ], "stop": [ 290231 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75532", "query_cov": 1.0, "subject_cov": 0.4189189189189189, "identity": 1.0, "score": 187.0, "evalue": 2.17e-58, "gene_start": 1, "gene_end": 93, "reference_start": 1, "reference_end": 93, "reference_sequence": "MISAKEQAKKGQIIYRYILLKIQSFKWPAGTRIFSERQLEIRFASSRSLIRTILNTLLGKDLIRHTKGNAGYFVAKNAGFSFFHKTQDNKLPKWAKLSTLMKNHLREVDRDVFSSIDSGVDFGNFKGLEAKLFDENKKNFLNLSFFGKDDVLQIFSEQNLQEQFFKDFAYNGIVVERKSSLICVDDETKNLLIYDLFYDDNNKFIVAMRSEYLNPRIKIINA" }, "paralog": false, "identity": 0.9948453608247423, "score": 381.0, "evalue": 3.29e-137, "description": "Internal stop codon at 290231. Nonsense mutation around 290231.", "psc": { "uniref90_id": "UniRef90_P75532", "gene": "MngR", "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog", "uniref50_id": "UniRef50_P75532", "cog_id": "COG2188", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003700" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75532", "UniRef:UniRef50_P75532", "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700" ] }, "pscc": { "uniref50_id": "UniRef50_P75532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75532" ], "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog" } }, "id": "GIHJHMOALN_546", "locus": "GCA009939745_00511" }, { "type": "cds", "sequence": "contig_1", "start": 290252, "stop": 290620, "strand": "+", "frame": 2, "gene": "mngR", "product": "putative HTH-type transcriptional regulator MG101-like protein", "db_xrefs": [ "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700", "SO:0001217", "UniRef:UniRef50_P75532", "UniRef:UniRef90_P75532" ], "nt": "ATGAAGAACCACCTACGCGAAGTTGACCGTGATGTTTTCAGCTCAATTGACAGTGGCGTTGACTTTGGTAACTTTAAGGGTTTGGAAGCCAAGTTATTTGATGAAAACAAGAAGAATTTTTTAAACCTTAGCTTCTTTGGCAAGGATGATGTGCTCCAAATTTTTAGCGAGCAAAACCTCCAGGAACAATTCTTTAAGGACTTTGCTTACAACGGCATTGTGGTAGAGCGCAAAAGCTCCTTAATCTGTGTGGATGATGAGACTAAGAACTTACTGATCTACGACCTCTTTTATGATGACAACAATAAGTTCATAGTAGCAATGCGCTCTGAATATTTAAACCCCCGCATCAAAATTATTAATGCTTAA", "aa": "MKNHLREVDRDVFSSIDSGVDFGNFKGLEAKLFDENKKNFLNLSFFGKDDVLQIFSEQNLQEQFFKDFAYNGIVVERKSSLICVDDETKNLLIYDLFYDDNNKFIVAMRSEYLNPRIKIINA", "aa_hexdigest": "4af42ae1fda77222ae6c7c66b9bf9861", "start_type": "ATG", "rbs_motif": null, "genes": [ "mngR" ], "truncated": "5-prime", "pseudogene": { "start": 289952, "stop": 290620, "observations": { "mutation": [ 290231 ], "stop": [ 290231 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75532", "query_cov": 1.0, "subject_cov": 0.5495495495495496, "identity": 1.0, "score": 246.0, "evalue": 2.77e-81, "gene_start": 1, "gene_end": 122, "reference_start": 101, "reference_end": 222, "reference_sequence": "MISAKEQAKKGQIIYRYILLKIQSFKWPAGTRIFSERQLEIRFASSRSLIRTILNTLLGKDLIRHTKGNAGYFVAKNAGFSFFHKTQDNKLPKWAKLSTLMKNHLREVDRDVFSSIDSGVDFGNFKGLEAKLFDENKKNFLNLSFFGKDDVLQIFSEQNLQEQFFKDFAYNGIVVERKSSLICVDDETKNLLIYDLFYDDNNKFIVAMRSEYLNPRIKIINA" }, "paralog": false, "identity": 0.9954954954954955, "score": 437.0, "evalue": 8.43e-159, "description": "Internal stop codon at 290231. Nonsense mutation around 290231.", "psc": { "uniref90_id": "UniRef90_P75532", "gene": "MngR", "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog", "uniref50_id": "UniRef50_P75532", "cog_id": "COG2188", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003700" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75532", "UniRef:UniRef50_P75532", "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700" ] }, "pscc": { "uniref50_id": "UniRef50_P75532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75532" ], "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog" } }, "id": "GIHJHMOALN_547", "locus": "GCA009939745_00512" }, { "type": "cds", "sequence": "contig_1", "start": 290613, "stop": 291560, "strand": "+", "frame": 3, "gene": "trxB", "product": "thioredoxin-disulfide reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "EC:1.8.1.9", "GO:0004791", "GO:0005737", "GO:0019430", "GO:0071949", "RefSeq:WP_014325415.1", "SO:0001217", "UniParc:UPI0001DD64F9", "UniRef:UniRef100_A0AAV5N8K6", "UniRef:UniRef50_P47348", "UniRef:UniRef90_P75531" ], "nt": "ATGCTTAAAGTAAAGTCAGATTTTTTAACTAAAGATCAGGTTATTTACGATGTAGCGATAGTGGGTGCTGGGCCAGCTGGCATAGCAGCGGGTATTTATGGCAAACGCGCTAACCTCAATCTAGCCATTATTGAAGGTAGTACTCCCGGCGGTAAGGTTGTCAAAACCAACATTGTCGAGAACTATCCGGGTTACAAGTCAATTACCGGACCTGACTTGGGGTTGGAAATGTACAACCACCTAATTGATTTAGAGCCCACCTTTTTCTATGCTAACCTAATTAAGTTAGATAAGGCAGCAGATACCTTTATCTTGTACCTCGATGACAAAACTGTGGTGTTTGCCAAAACCGTTATTTACGCTACGGGAATGTTGGAGCGTAAACTGGGCGTAGCCAAAGAAGACCACTTTTACGGTAAGGGAATTAGTTACTGTGCCATTTGTGATGGGTCCTTGTACAAAGACCAGGTGGTGGGTGTTGTTGGTGGGGGGAACTCTGCCATTCAGGAAGCGTTGTATTTAGCTGGCATGGCTAAAACGGTGCACTTAATCCACCGCCGTGAGGGCTTTCGTGCTGATGAAACGGCGTTAAATAAACTGAGAAACTTACCGAATGTTGTTTTTCACTTGAACTATACCGTAAAAGAATTGTTGGGTAACAATACCCTAAACGGCATAGTGCTTCAAAACACGCTCGATCATTCAACAAAACAGATAGATCTCAATTGTGTCTTTCCCTATATTGGTTTTGAAAGTATTACCAAACCGGTGGAACACCTCAACTTAAAGTTAGATCCCCAGGGTTTTTTAATTACCAATGAACAGATGGAAACAAGTTTAAAGGGTCTGTTTGCAGCAGGTGATTGTCGTTCTAAGCACTTCCGTCAAATTGGTACTGCCATTAATGACGGCATTATCGCGGTGTTAACGATCCGTGATGTCCTTTAG", "aa": "MLKVKSDFLTKDQVIYDVAIVGAGPAGIAAGIYGKRANLNLAIIEGSTPGGKVVKTNIVENYPGYKSITGPDLGLEMYNHLIDLEPTFFYANLIKLDKAADTFILYLDDKTVVFAKTVIYATGMLERKLGVAKEDHFYGKGISYCAICDGSLYKDQVVGVVGGGNSAIQEALYLAGMAKTVHLIHRREGFRADETALNKLRNLPNVVFHLNYTVKELLGNNTLNGIVLQNTLDHSTKQIDLNCVFPYIGFESITKPVEHLNLKLDPQGFLITNEQMETSLKGLFAAGDCRSKHFRQIGTAINDGIIAVLTIRDVL", "aa_hexdigest": "cd6e1a03f59190c61bc4a710042cc1c1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64F9", "ncbi_nrp_id": "WP_014325415.1", "uniref100_id": "UniRef100_A0AAV5N8K6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64F9", "RefSeq:WP_014325415.1", "UniRef:UniRef100_A0AAV5N8K6" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N8K6", "uniref90_id": "UniRef90_P75531" }, "psc": { "uniref90_id": "UniRef90_P75531", "gene": "trxB", "product": "thioredoxin-disulfide reductase", "ec_ids": [ "1.8.1.9" ], "uniref50_id": "UniRef50_P47348", "cog_id": "COG0492", "cog_category": "O", "go_ids": [ "GO:0004791", "GO:0005737", "GO:0019430", "GO:0071949" ] }, "pscc": { "uniref50_id": "UniRef50_P47348", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47348" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "GIHJHMOALN_548", "locus": "GCA009939745_00513" }, { "type": "cds", "sequence": "contig_1", "start": 291706, "stop": 292392, "strand": "+", "frame": 1, "gene": "whiA", "product": "putative cell division protein WhiA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47349" ], "nt": "TTGTTTAGCAATGCACTCCAGTTTAGTTTTCAAATCCACGAAACGAGCACTCACTGTGAGTTTAGTTTTAGGGCTTCGCAAACAGAGGTGGATCAGTTGTTAGCCTTTGATGCAACTCAGAGCGATTTACCGCTACAGAAGGCCTATGTCATTGGTGCCTTTTTGAGTGGTGGTAGTGTGAGCGATTTACTCCATTCCAGTAACTTTCACCTCCAAATTAGCACTAACAACGAACTGCAAATAAAACAGTTAATGAAGCTGTTTCATCCTTTTAAACAAACCACTAAGCGCCACCAGCTTTTGGTCTATGTTAAAAGCTATCAGGCCATTTGTGACTTCTTTAAACTAGTCGAAGCTTTTGATGGTTATCTAGCCTTTGAAGAAAACCAATTGCAAAAAAATTTTGCCTTAGAACAAGTGCGTAAAAGTAACTTAGAAATTGCTAACCTAATGCGCACTTTAAAGACCAGCACAAGCACAGCGGAACAGCTAAAAATCCTCATAAACAGTGCTGACTTTAAAAAGCAATCGCTCAACTTTCAGCGCTTTTGTTTAGTCAAACTAGACCATCCTGACTGGTCATTAGAACAAATTGCCCAGTTTTTTGAAAGGAAATACAAAGTGCAAATTACCCGTTCGGGAATTCAACACCTCAACGCTAAGTTAAAAAAACTAAACCAAAATTAA", "aa": "MFSNALQFSFQIHETSTHCEFSFRASQTEVDQLLAFDATQSDLPLQKAYVIGAFLSGGSVSDLLHSSNFHLQISTNNELQIKQLMKLFHPFKQTTKRHQLLVYVKSYQAICDFFKLVEAFDGYLAFEENQLQKNFALEQVRKSNLEIANLMRTLKTSTSTAEQLKILINSADFKKQSLNFQRFCLVKLDHPDWSLEQIAQFFERKYKVQITRSGIQHLNAKLKKLNQN", "aa_hexdigest": "d398808f0140b4e45111776700f91edf", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47349", "query_cov": 0.9868421052631579, "subject_cov": 0.8035714285714286, "identity": 0.569, "score": 258.0, "evalue": 3.35e-83, "valid": false, "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_P47349", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_P47349", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47349" ], "product": "Probable cell division protein WhiA" }, "genes": [ "whiA" ], "id": "GIHJHMOALN_549", "locus": "GCA009939745_00514" }, { "type": "cds", "sequence": "contig_1", "start": 292425, "stop": 292655, "strand": "+", "frame": 3, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020", "RefSeq:WP_010874599.1", "SO:0001217", "UniParc:UPI00001357BB", "UniRef:UniRef100_Q9EXD0", "UniRef:UniRef50_P58061", "UniRef:UniRef90_Q9EXD0" ], "nt": "ATGGACGCAATTCAAATAGTAATGTTTGTAATGGCAATCCTTTGTTTAATCATCGGTTTATTGCTATCCAACCATGGTTCTACTGGCGGACTTGCTTCGCTTTCAGGTCAAGATTTGGAAATTTTTCGCAAAACCAAAGACCGCGGAATTGTCAAAATTCTCCAAATTACGATGTTTATTTTAGTAGTGCTTTTTCTTATTTTAGGGTTAGTTTTTCATTTTGCGCTTTAA", "aa": "MDAIQIVMFVMAILCLIIGLLLSNHGSTGGLASLSGQDLEIFRKTKDRGIVKILQITMFILVVLFLILGLVFHFAL", "aa_hexdigest": "45076910ab79b362bfaf44991ea0252c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001357BB", "ncbi_nrp_id": "WP_010874599.1", "uniref100_id": "UniRef100_Q9EXD0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001357BB", "RefSeq:WP_010874599.1", "UniRef:UniRef100_Q9EXD0" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD0", "uniref90_id": "UniRef90_Q9EXD0" }, "psc": { "uniref90_id": "UniRef90_Q9EXD0", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_P58061", "go_ids": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P58061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P58061" ], "product": "Probable protein-export membrane protein SecG" }, "genes": [ "secG" ], "id": "GIHJHMOALN_550", "locus": "GCA009939745_00515" }, { "type": "cds", "sequence": "contig_1", "start": 292666, "stop": 292827, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTATTAACTGATTTACAAAAGCGAATCTTCGCGATTGTGAAAAAAGAGAACGGCAAACCTATTCCTCCGGGAATAGTTGTCAGGATGATGGAGAATCAAGCTGGTTTTCCGGGAAAGCAACAGGTATACAGAGCAATTGATGACTTGTTAGAATGA", "aa": "MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLE", "aa_hexdigest": "29b02c844d00215c9ae3be8493903c03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6025.118200000003, "isoelectric_point": 9.599229621887208 }, "id": "GIHJHMOALN_551", "locus": "GCA009939745_00516" }, { "type": "cds", "sequence": "contig_1", "start": 292897, "stop": 293844, "strand": "+", "frame": 1, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_P47350", "UniRef:UniRef90_P75529" ], "nt": "TTGGATCAAAAATTTCAGGGCATTCTCAATTTAGGTAACAAGAACACTGGTTTTGTCCGTCCCTTAGATGATGACAAAACGGTGTACTATATCCATTTTTCCAACCTAGCGGGAGCACTGGATGGTGATCTAGTTGAATTCTGTCCGCTCGACAAACCACAGGTAGGTGATAAGTTTGATGCTGCAGTGCTCAAAATTGTGAAGCGCTCCCGGGTGCTGTACGCTGGTAACTTTTTGATTGAGTACTCCGATTTTGGTCAGGAGTTTCGCATTGTTGCTGACAATCCTCGTTTTTACCTCACCCCGATTGTCAATAAGGCCTCAGTTCCAGCTGAACTGGAGAGCAATACTAAGGTCGCTTTTCAAATTGATGAGTATGATCCTGCAAATAACCTGTGTAAAGTCTCCATTCAGCAAATCTTGGGGAATAACGATGAACCGTTAATTAACCTCAAGGCGATTATGTTAGACCACTCGATTGTCTTTGAAAAGAATGATGTGGTCGAACAACAAGCAGCGAAGTTACAGTTTGATGAAAAAGAGCAGAGTAAGCCCTACCGCAAGGACTTAACCGAACTTGCCTTTGTCACGATTGACCCCGCCACTTCCAAGGACTTGGATGATGCGATTTACGTCAAGCGCACCGACAAAGGTTTTGTTTTGTATGTTGCCATTGCTGATGTGGCTTACTATGTGCAGCGCAACAGTGAACTTGACATTGAAGCCCGTCATAAAACAAGCTCAATTTACCTCCCGGGATACTATGTGGTACCGATGTTACCAGAACGTTTATCAAACGAGCTGTGTTCTTTAAACCCAAATGAAAAGCGGTATGTGGTGGTGTGTGAGTTGAACTTTGATCATGAAGCACGCTTAAACTTTAGTGAAGTGTATCCCGCTACCATTGTCTCCCAACGTCGCTTTGCTTACAGTGAAGTTAATGATTGA", "aa": "MDQKFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDLVEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYSDFGQEFRIVADNPRFYLTPIVNKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFEKNDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVAIADVAYYVQRNSELDIEARHKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCELNFDHEARLNFSEVYPATIVSQRRFAYSEVND", "aa_hexdigest": "ed2d343e9e2f9c6656a2befbd9af0dfb", "start_type": "TTG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "both", "pseudogene": { "start": 292666, "stop": 294147, "observations": { "mutation": [ 292825, 293842, 294091 ], "stop": [ 292825, 293842, 294091 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75529", "query_cov": 1.0, "subject_cov": 0.43388429752066116, "identity": 0.9940000000000001, "score": 621.0, "evalue": 1.65e-218, "gene_start": 1, "gene_end": 315, "reference_start": 78, "reference_end": 392, "reference_sequence": "MKVLTDLQKRIFAIVKKENGKPIPPGIVVRMMENQAGFPGKQQVYRAIDDLLEWHIFRKSGGATNQLLINYELADPVLDQKFQGILNLGNKNTGFVRPLDDDKTVYYIHFSNLAGALDGDLVEFCPLDKPQVGDKFDAAVLKIVKRSRVLYAGNFLIEYSDFGQEFRIVADNPRFYLTPIVNKASVPAELESNTKVAFQIDEYDPANNLCKVSIQQILGNNDEPLINLKAIMLDHSIVFEDNDVVEQQAAKLQFDEKEQSKPYRKDLTELAFVTIDPATSKDLDDAIYVKRTDKGFVLYVAIADVAYYVQRNSELDIEARHKTSSIYLPGYYVVPMLPERLSNELCSLNPNEKRYVVVCELNFDHEARLNFSEVYPATIVSQRRFAYSEVNDWLEDSDALKDESATVLESLKAGFTLSELIAEQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVAWTLTNHKVHLPYRVHPRPSKKKLQMLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVILLLRTLGKAFYIVNPLIHFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTEREQLNAELEQICETINDTEIKINGCERTANDYLTTLYLSKQVGQTFHGFISAITSFGIFMRMDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKRAILTLV" }, "paralog": false, "identity": 0.9918864097363083, "score": 965.0, "evalue": 0.0, "description": "Internal stop codon at 292825, 293842, 294091. Nonsense mutation around 292825, 293842, 294091.", "psc": { "uniref90_id": "UniRef90_P75529", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_P47350", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75529", "EC:3.1.13.1", "UniRef:UniRef50_P47350", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ] }, "pscc": { "uniref50_id": "UniRef50_P47350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47350" ], "product": "Ribonuclease R" } }, "id": "GIHJHMOALN_552", "locus": "GCA009939745_00517" }, { "type": "cds", "sequence": "contig_1", "start": 293914, "stop": 294093, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00773.24" ], "nt": "TTGAGCGAATTGATTGCTGAACAGCGCAAAAAGAAGGGGACTATTGACCTATCTCACAGTGAAACAGAAGTAGTCGTTGATCAAAACTACTACCCGATTGAGATCCGTTTTCTCACCCACGGCAAGGCGGAAACAATGATCGAAAACCTAATGGTGGTTGCCAATGAAGCGGTGGCTTGA", "aa": "MSELIAEQRKKKGTIDLSHSETEVVVDQNYYPIEIRFLTHGKAETMIENLMVVANEAVA", "aa_hexdigest": "077f519de39ec05950be0135225b340a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00773.24", "name": "RNB", "length": 59, "aa_cov": 0.9830508474576272, "hmm_cov": 1.0, "evalue": 2.3706750695819954e-10, "score": 35.65190887451172, "start": 2, "stop": 59 } ], "seq_stats": { "molecular_weight": 6691.574899999998, "isoelectric_point": 4.951040077209471 }, "id": "GIHJHMOALN_553", "locus": "GCA009939745_00518" }, { "type": "cds", "sequence": "contig_1", "start": 294163, "stop": 294846, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00773.24", "PFAM:PF00575.29" ], "nt": "ATGTTGTTGGAAAACATTGTCGAGCTCAAAATTACCCAACCAAACTTTGTACTCGATACGGTCACTTCCACCCAAATTGCGGCTTGGTTAAAGGAAAATAAGGACAACCCGAGCTATGACATCTTTGTCATCTTGTTATTAAGAACGCTAGGTAAAGCCTTTTACATTGTTAATCCACTGATACACTTTAGCATTGGTTCACACCACTATACCCATTTCACTTCGCCCATCCGTCGCTATGCTGATTTAACGGTACACCGTTTGTTGTGGATGAATCTGTTTACTCCAGAGCGTTTTACTGACACTGAGCGTGAACAGCTCAACGCCGAGCTAGAACAAATTTGTGAAACCATTAACGATACTGAGATTAAGATTAATGGTTGTGAACGGACTGCCAACGATTACTTAACTACGCTGTATTTGTCTAAACAAGTGGGACAAACTTTCCACGGTTTTATTTCCGCTATTACTAGTTTTGGCATCTTTATGCGAATGGATGAAAACAACTTTGATGGACTTATTAAGATTACCTCCATTCCCGAAGACTTCTTTGTCTTTGAAAAGGATCGGATGGTGTTGCGCGGCAAACGCACCAACAAGGTTTTTAGAATTGGTGATAGGTTAACCGCGAAACTGACTGAAATTGATACAGTGCAGAAACGCGCCATTCTAACCCTGGTTTAA", "aa": "MLLENIVELKITQPNFVLDTVTSTQIAAWLKENKDNPSYDIFVILLLRTLGKAFYIVNPLIHFSIGSHHYTHFTSPIRRYADLTVHRLLWMNLFTPERFTDTEREQLNAELEQICETINDTEIKINGCERTANDYLTTLYLSKQVGQTFHGFISAITSFGIFMRMDENNFDGLIKITSIPEDFFVFEKDRMVLRGKRTNKVFRIGDRLTAKLTEIDTVQKRAILTLV", "aa_hexdigest": "bdab34d4f3f66ca21b8330091a27f971", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00773.24", "name": "RNB", "length": 75, "aa_cov": 0.32599118942731276, "hmm_cov": 0.9466666666666667, "evalue": 4.14733016061755e-21, "score": 71.00458526611328, "start": 20, "stop": 93 }, { "id": "PF00575.29", "name": "S1", "length": 82, "aa_cov": 0.36123348017621143, "hmm_cov": 0.8536585365853658, "evalue": 2.142304010921419e-11, "score": 39.560325622558594, "start": 144, "stop": 225 } ], "seq_stats": { "molecular_weight": 26401.13100000002, "isoelectric_point": 6.312959098815918 }, "id": "GIHJHMOALN_554", "locus": "GCA009939745_00519" }, { "type": "cds", "sequence": "contig_1", "start": 294846, "stop": 295454, "strand": "+", "frame": 3, "gene": "cdaM", "product": "diadenylate cyclase CdaM", "db_xrefs": [ "COG:COG1624", "COG:T", "EC:2.7.7.85", "GO:0004016", "GO:0005524", "GO:0005886", "GO:0006171", "GO:0106408", "KEGG:K18672", "RefSeq:WP_010874601.1", "SO:0001217", "UniParc:UPI000013928C", "UniRef:UniRef100_P75528", "UniRef:UniRef50_P47351", "UniRef:UniRef90_P75528" ], "nt": "ATGATGACAGTGGAAGTCTTTTCGGTTATTATCCTTGTCCTTTTATTCTTAATCTTAGCGCTTACCTTGTTGTTTGTTTTACTCAACAAGCGTACTAGAAGCTTTGTTATTCGTACCTTTACTGGATTATTTAGATCAAAGCATACTACCAGTCAAAAGAACTTTTATGACAACTTGACCTCAACCTTACTGAGGTTATCAACTGATAAGATTGGTGCCATTATTGCCATTGAAAACCAGGACTCACTCGAGTCTTACGTCAATATTGGCTACCGCGTGACTTCGGACTTTTCCCCCGAGTTGTTAGTAACGATCTTTTACAACAAGCAGTCGCCACTTCACGATGGAGCGGTAATTGTGCGGGACTACCAGATTGTGAGTGTGTCCTCTTACTTCCCAATGACACGGCAACTAATTGATGTGTCATACGGTTCCAGACACCGCAGCGCGTTAGGTCTAACCGAAAAGTGTGATGCCATAGTCTTTATTGTTAGTGAAACAACAGGCAAAATTTCGGTGGCTGTGCGCGGCGTGATTAAAACACTGTCGAGTAACTCCGACCGTTTACAAGACCAGATTATTCACTATCTGACGGTAAAGCCGGGTTAA", "aa": "MMTVEVFSVIILVLLFLILALTLLFVLLNKRTRSFVIRTFTGLFRSKHTTSQKNFYDNLTSTLLRLSTDKIGAIIAIENQDSLESYVNIGYRVTSDFSPELLVTIFYNKQSPLHDGAVIVRDYQIVSVSSYFPMTRQLIDVSYGSRHRSALGLTEKCDAIVFIVSETTGKISVAVRGVIKTLSSNSDRLQDQIIHYLTVKPG", "aa_hexdigest": "bd9fb3567248746504c0b4dd43ffbc94", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013928C", "ncbi_nrp_id": "WP_010874601.1", "uniref100_id": "UniRef100_P75528", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013928C", "RefSeq:WP_010874601.1", "UniRef:UniRef100_P75528" ] }, "ips": { "uniref100_id": "UniRef100_P75528", "uniref90_id": "UniRef90_P75528" }, "psc": { "uniref90_id": "UniRef90_P75528", "gene": "cdaM", "product": "diadenylate cyclase CdaM", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_P47351", "cog_id": "COG1624", "cog_category": "T", "kegg_orthology_id": "K18672", "go_ids": [ "GO:0005886", "GO:0004016", "GO:0005524", "GO:0106408", "GO:0006171" ] }, "pscc": { "uniref50_id": "UniRef50_P47351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47351" ], "product": "Diadenylate cyclase" }, "genes": [ "cdaM" ], "id": "GIHJHMOALN_555", "locus": "GCA009939745_00520" }, { "type": "cds", "sequence": "contig_1", "start": 295600, "stop": 295785, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01327.26" ], "nt": "ATGTTCTACATCCACCTAATGGATGGTGGGGTGGAACACAAGTGTCTGTTAATTAATCCCAAGATTATCAACTTAAGTGCCAACAAATCCTTTTTAAAGAGCGGTGAGGGTTGTTTGAGTGTTCCCAAAATGCACCAGGGTTATGTTATTCGCCACGAATGGATTACGATCACTGGTTTTGATTGA", "aa": "MFYIHLMDGGVEHKCLLINPKIINLSANKSFLKSGEGCLSVPKMHQGYVIRHEWITITGFD", "aa_hexdigest": "159f11430901299627617eef6d196b42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01327.26", "name": "Pep_deformylase", "length": 63, "aa_cov": 1.0, "hmm_cov": 0.9523809523809523, "evalue": 3.567415863977442e-13, "score": 44.89892578125, "start": 1, "stop": 61 } ], "seq_stats": { "molecular_weight": 6932.0999, "isoelectric_point": 7.846785545349122 }, "id": "GIHJHMOALN_556", "locus": "GCA009939745_00521" }, { "type": "cds", "sequence": "contig_1", "start": 295792, "stop": 295974, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTGGACGATGTTAAGGAAATTAACGAACCGACTAAACCAGTTCAGTTTCCCTTAGACCAAGCGAGTCTCGATTGCATTGCCAAAATGATGGCTTACGTTGATGCATCTTACAACGGTGATGCTGAGAAGTATGGCATTATTCCCGGCATTGGGATTGCCGCTAACCAAATTGGTTATTGA", "aa": "MLDDVKEINEPTKPVQFPLDQASLDCIAKMMAYVDASYNGDAEKYGIIPGIGIAANQIGY", "aa_hexdigest": "9066c405d33e14da462147f6c20165a3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6507.353900000002, "isoelectric_point": 4.122554969787596 }, "id": "GIHJHMOALN_557", "locus": "GCA009939745_00522" }, { "type": "cds", "sequence": "contig_1", "start": 296045, "stop": 296266, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33", "PFAM:PF00625.27" ], "nt": "ATGGTCGATACAGGGAGAATTTTTGTCATTACCGGACCGAGCGGTGTTGGCAAAAGTAGCCTTGTTAGATGTTTAATTGACCATTTCAAAGATAAACTGCGCTACAGCATTTCCGCTACCACCCGTAAAATGCGTAACAGCGAAACTGAGGGTGTGGATTACTTCTTTAAAGATAAAGCGGAGTTTGAAAAACTGATTGCTGCTGATGCCTTTGTGGAGTGA", "aa": "MVDTGRIFVITGPSGVGKSSLVRCLIDHFKDKLRYSISATTRKMRNSETEGVDYFFKDKAEFEKLIAADAFVE", "aa_hexdigest": "bb55ab2126a223b29d544b78bb1acdbb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 67, "aa_cov": 0.8767123287671232, "hmm_cov": 1.0, "evalue": 1.0562812905888031e-07, "score": 28.087451934814453, "start": 2, "stop": 65 }, { "id": "PF00625.27", "name": "Guanylate_kin", "length": 68, "aa_cov": 0.9315068493150684, "hmm_cov": 1.0, "evalue": 2.876532877103215e-19, "score": 64.94884490966797, "start": 6, "stop": 73 } ], "seq_stats": { "molecular_weight": 8237.359199999997, "isoelectric_point": 7.869220542907715 }, "id": "GIHJHMOALN_558", "locus": "GCA009939745_00523" }, { "type": "cds", "sequence": "contig_1", "start": 296270, "stop": 296614, "strand": "+", "frame": 2, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "COG:COG0194", "COG:F", "EC:2.7.4.8", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163", "SO:0001217", "UniRef:UniRef50_P47353", "UniRef:UniRef90_P75526" ], "nt": "ATGTATAACGATAACTACTATGGTACCCTTAAATCCCAAGCTGAACAAATTATTCACAACGGTGGCAACTTGGTGTTAGAAATTGAATATCAAGGTGCTTTACAAGTTAAGCAAAAGTATCCCAATGATGTGGTGCTAATTTTTATTAAACCACCTTCAATGGAAGAGTTGTTAGTGCGCTTAAAGAAGCGTAATGACGAGGATGCAATAACAATTCAAAACCGATTGAAACAAGCTGAGAAGGAATGTCAACAAATTGGTCACTTTAAGTATGTGGTTACCAACAACGAGTTTGACAAGACCTTAGCGGAGTTACAAGCAATTTTACTAGCTGAATTTAATTAA", "aa": "MYNDNYYGTLKSQAEQIIHNGGNLVLEIEYQGALQVKQKYPNDVVLIFIKPPSMEELLVRLKKRNDEDAITIQNRLKQAEKECQQIGHFKYVVTNNEFDKTLAELQAILLAEFN", "aa_hexdigest": "b9b1766fd16e18151b3704ab3886d132", "start_type": "ATG", "rbs_motif": null, "genes": [ "gmk" ], "truncated": "5-prime", "pseudogene": { "start": 295970, "stop": 296614, "observations": { "mutation": [ 296264 ], "stop": [ 296264 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75526", "query_cov": 1.0, "subject_cov": 0.4769874476987448, "identity": 1.0, "score": 224.0, "evalue": 1.62e-72, "gene_start": 1, "gene_end": 114, "reference_start": 126, "reference_end": 239, "reference_sequence": "MQSRLAWSKGNWTGLVGSLISLTSSSTSHALVGNNSILICGKIKIILNIEMVDTGRIFVITGPSGVGKSSLVRCLIDHFKDKLRYSISATTRKMRNSETEGVDYFFKDKAEFEKLIAADAFVEWAMYNDNYYGTLKSQAEQIIHNGGNLVLEIEYQGALQVKQKYPNDVVLIFIKPPSMEELLVRLKKRNDEDAITIQNRLKQAEKECQQIGHFKYVVTNNEFDKTLAELQAILLAEFN" }, "paralog": false, "identity": 0.9953271028037384, "score": 419.0, "evalue": 1.15e-151, "description": "Internal stop codon at 296264. Nonsense mutation around 296264.", "psc": { "uniref90_id": "UniRef90_P75526", "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_P47353", "cog_id": "COG0194", "cog_category": "F", "go_ids": [ "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75526", "EC:2.7.4.8", "UniRef:UniRef50_P47353", "COG:COG0194", "COG:F", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_P47353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47353" ], "product": "Guanylate kinase" } }, "id": "GIHJHMOALN_559", "locus": "GCA009939745_00524" }, { "type": "cds", "sequence": "contig_1", "start": 296614, "stop": 296988, "strand": "+", "frame": 1, "gene": "pTC1", "product": "Putative protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "GO:0017018", "GO:0033192", "GO:0140791", "GO:0180004", "GO:0180005", "GO:0180006", "GO:0180007", "GO:0180008", "GO:1990439", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_P47354", "UniRef:UniRef90_P75525" ], "nt": "ATGGACAGCACCAACCAAAACCTGTTCGCGTCATTGTCCAAAAAAGGACTCGTTCGCAAAGAAAATCAGGACTTTAGTGTTGTTACTTTCAACCGCTTTGGTCAGTTAATGAGCCTTGTTTGTGATGGTTTAGGCGGTTATAAGGGCGGTAAGATGGCTAGTGCCTTGGTGAGTGAAGTTTTTACCAAGTCCTTTACAGTCTTTGATTTTCATAGTCAAACTGAAAGGGCAGTGAAACAGTGGTTTGAAATTACTTTAATTGAAGCGCGGCGCACCTTAGAACAGTACTTTCAAACAATTAAACGTAACCAAGTCCAGTTTGCGCGAATGGCGACTACCTTAGTGTTGAGTATTATAAGTAAGCAAAACATTTGA", "aa": "MDSTNQNLFASLSKKGLVRKENQDFSVVTFNRFGQLMSLVCDGLGGYKGGKMASALVSEVFTKSFTVFDFHSQTERAVKQWFEITLIEARRTLEQYFQTIKRNQVQFARMATTLVLSIISKQNI", "aa_hexdigest": "7160d456dad552a1d881329e44c14a79", "start_type": "ATG", "rbs_motif": null, "genes": [ "pTC1" ], "truncated": "3-prime", "pseudogene": { "start": 296614, "stop": 297291, "observations": { "mutation": [ 296986 ], "stop": [ 296986 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75525", "query_cov": 1.0, "subject_cov": 0.47876447876447875, "identity": 0.992, "score": 237.0, "evalue": 3.16e-77, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MDSTNQNLFASLSKKGPVRKENQDFSVVTFNRFGQLMSLVCDGLGGYKGGKMASALVSEVFTKSFTVFDFHSQTERAVKQWFEITLIEARRTLEQYFQTIKRNQVQFARMATTLVLSIISKQNIWTFWVGDSRAYLINSYQSLQITEDHNLYNQLLQMHATPDVIASYKDKLLALTATVSKDQERQLKYSFRCDVVNAWDFLLLCSDGLYNFLDPNCFYEVITSAPNLKKAVTQLAKLSLDNASNDNITLNLINLKQWH" }, "paralog": false, "identity": 0.9911111111111112, "score": 443.0, "evalue": 8.96e-161, "description": "Internal stop codon at 296986. Nonsense mutation around 296986.", "psc": { "uniref90_id": "UniRef90_P75525", "gene": "PTC1", "product": "Putative protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_P47354", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "go_ids": [ "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75525", "EC:3.1.3.16", "UniRef:UniRef50_P47354", "COG:COG0631", "COG:T", "KEGG:K20074", "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ] }, "pscc": { "uniref50_id": "UniRef50_P47354", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47354" ], "product": "Putative protein phosphatase" } }, "id": "GIHJHMOALN_560", "locus": "GCA009939745_00525" }, { "type": "cds", "sequence": "contig_1", "start": 296998, "stop": 297393, "strand": "+", "frame": 1, "gene": "pTC1", "product": "Putative protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "GO:0017018", "GO:0033192", "GO:0140791", "GO:0180004", "GO:0180005", "GO:0180006", "GO:0180007", "GO:0180008", "GO:1990439", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_P47354", "UniRef:UniRef90_P75525" ], "nt": "GTGGGTGATTCACGTGCTTATTTAATTAACAGTTACCAAAGCTTGCAAATTACCGAAGACCATAACCTGTACAACCAACTGTTACAGATGCACGCTACCCCTGATGTGATTGCCTCTTATAAAGACAAGCTGTTAGCCCTCACTGCTACCGTTTCCAAAGATCAGGAACGCCAGTTGAAATACAGCTTTAGGTGTGATGTAGTAAACGCTTGGGACTTTTTACTGTTGTGCTCTGATGGTCTGTACAACTTCTTGGATCCCAACTGCTTTTATGAGGTCATTACTAGTGCACCTAACCTCAAAAAGGCCGTTACACAACTAGCAAAGTTGAGCTTGGATAACGCTAGTAATGACAACATTACGCTCAACTTGATTAATTTAAAACAATGGCACTAA", "aa": "MGDSRAYLINSYQSLQITEDHNLYNQLLQMHATPDVIASYKDKLLALTATVSKDQERQLKYSFRCDVVNAWDFLLLCSDGLYNFLDPNCFYEVITSAPNLKKAVTQLAKLSLDNASNDNITLNLINLKQWH", "aa_hexdigest": "cdfd83a2d804c7344bee24f8c1941817", "start_type": "GTG", "rbs_motif": null, "genes": [ "pTC1" ], "truncated": "5-prime", "pseudogene": { "start": 296698, "stop": 297393, "observations": { "mutation": [ 296986 ], "stop": [ 296986 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75525", "query_cov": 1.0, "subject_cov": 0.5057915057915058, "identity": 0.992, "score": 265.0, "evalue": 4.78e-88, "gene_start": 1, "gene_end": 131, "reference_start": 129, "reference_end": 259, "reference_sequence": "MDSTNQNLFASLSKKGPVRKENQDFSVVTFNRFGQLMSLVCDGLGGYKGGKMASALVSEVFTKSFTVFDFHSQTERAVKQWFEITLIEARRTLEQYFQTIKRNQVQFARMATTLVLSIISKQNIWTFWVGDSRAYLINSYQSLQITEDHNLYNQLLQMHATPDVIASYKDKLLALTATVSKDQERQLKYSFRCDVVNAWDFLLLCSDGLYNFLDPNCFYEVITSAPNLKKAVTQLAKLSLDNASNDNITLNLINLKQWH" }, "paralog": false, "identity": 0.9956709956709957, "score": 460.0, "evalue": 2.35e-167, "description": "Internal stop codon at 296986. Nonsense mutation around 296986.", "psc": { "uniref90_id": "UniRef90_P75525", "gene": "PTC1", "product": "Putative protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_P47354", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "go_ids": [ "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75525", "EC:3.1.3.16", "UniRef:UniRef50_P47354", "COG:COG0631", "COG:T", "KEGG:K20074", "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004" ] }, "pscc": { "uniref50_id": "UniRef50_P47354", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47354" ], "product": "Putative protein phosphatase" } }, "id": "GIHJHMOALN_561", "locus": "GCA009939745_00526" }, { "type": "cds", "sequence": "contig_1", "start": 297384, "stop": 298352, "strand": "+", "frame": 3, "gene": "sPS1", "product": "Putative serine/threonine-protein kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "EC:2.7.11.1", "GO:0004676", "GO:0004677", "GO:0004679", "GO:0004711", "GO:0005524", "GO:0035175", "GO:0035402", "GO:0035403", "GO:0035979", "GO:0044022", "GO:0044023", "GO:0044024", "GO:0044025", "GO:0072354", "GO:0072371", "GO:0072518", "GO:0106310", "GO:0140823", "GO:0140855", "GO:0140857", "GO:1990244", "SO:0001217", "UniRef:UniRef50_P47355", "UniRef:UniRef90_P75524" ], "nt": "ATGGCACTAAATTTAAAGATTGGTGACATTGTCCAAAACAAGTACCGGATTGAAAAGCTAATCAACCGTGGCGGGATGAACTCTTACCTGTTTTTAGCGAGCAACCTCCACGTCCAGGAATTTGGTCCACTGCAAAAGCGCCAGTTTACCCGGTTGGTGTTAAAGGTAGTACAACGCACCGACAAGATTAACGACAACAACTGGAAGAAGTTTTTGGATGAAACCATTACCACGACACGGGTGTCACACAAAAATCTGGTGCAAACCTTTGATGTAGTTAGCCCCCGTTTAAGCGTTTTGAGTGAAAACCAGGTGATCGTTTTAGAGGACACGGTGATGATTGTAATGGAGTATGTTGATGGTCCTTCTTTGCGTGAAATGCTCAACCAGAAGGGTTACTTTAGTGTGCAGGAGGTGGTGTACTACTTTACAAAGCTAGTCAAAGTAATTAACTACCTCCACAGTTTTGAACACCAAATTATTCACCGTGATCTAAAGCCAGAAAACATCCTGTTTACGAGTAACTTGACTGACATTAAACTGCTTGACTTTGGCATTGCTTCCGCTGTTATTCGTAACGCTGAGAAAACTGAAGTGTTAACGGACGAAAACTCGTTGTTTGGTACAGTTAGTTATATGACACCCGAAGTGTTGGAAAGTACTGTCAACAAAGAAGGCAAACGGATTCGTAAACCCCCTACAGTGCAGTATGACATCTATTCATTAGGAGTTATTTTGTTTGAAATGTTAGTCGGACGCGTACCGTTCAATAAATCGATTGATCCTAAAAAAGAACGGGAAACCATCCAAAAGGCGCGTAACTTTGATGTACCTTTAATGGGAAACTTGCGCAGTGATGTTCCGGTTAGTTTGGAAAACATTGTCTTTAAATGTACGGCTGTTAAACGTGAAAACAGTAAGTGGATGTATAGTGACACGAAGCAACTCTTGGCTGATTTGGCGCAATGA", "aa": "MALNLKIGDIVQNKYRIEKLINRGGMNSYLFLASNLHVQEFGPLQKRQFTRLVLKVVQRTDKINDNNWKKFLDETITTTRVSHKNLVQTFDVVSPRLSVLSENQVIVLEDTVMIVMEYVDGPSLREMLNQKGYFSVQEVVYYFTKLVKVINYLHSFEHQIIHRDLKPENILFTSNLTDIKLLDFGIASAVIRNAEKTEVLTDENSLFGTVSYMTPEVLESTVNKEGKRIRKPPTVQYDIYSLGVILFEMLVGRVPFNKSIDPKKERETIQKARNFDVPLMGNLRSDVPVSLENIVFKCTAVKRENSKWMYSDTKQLLADLAQ", "aa_hexdigest": "f8adeb2f37efb586f36c8ba3d21b2912", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75524", "query_cov": 1.0, "subject_cov": 0.8277634961439588, "identity": 0.997, "score": 625.0, "evalue": 4.83e-225, "valid": true, "gene": "SPS1", "product": "Putative serine/threonine-protein kinase", "ec_ids": [ "2.7.11.1" ], "uniref50_id": "UniRef50_P47355", "cog_id": "COG0515", "cog_category": "T", "go_ids": [ "GO:0004676", "GO:0004679", "GO:0005524", "GO:0004677", "GO:0044024", "GO:0072371", "GO:1990244", "GO:0035979", "GO:0044025", "GO:0140823", "GO:0035175", "GO:0044022", "GO:0140855", "GO:0035402", "GO:0072354", "GO:0140857", "GO:0035403", "GO:0044023", "GO:0106310", "GO:0072518", "GO:0004711" ] }, "pscc": { "uniref50_id": "UniRef50_P47355", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47355" ], "product": "Putative serine/threonine-protein kinase" }, "genes": [ "sPS1" ], "id": "GIHJHMOALN_562", "locus": "GCA009939745_00527" }, { "type": "cds", "sequence": "contig_1", "start": 298380, "stop": 298553, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCACGAACGTATTCTAGAAGGACAAAACGAAATGCGTGAACTAATGGTAAGTAATTACCTACCGTGGTATTTAAGGAAGGGTGTTTTGATCTTTTTTTCTGTTGTTTTATTAGCACTCTTAATTGCTGTAGTTAGCTTTTTTATTATTACTGGGGTAGTTGTCCATTCGTAA", "aa": "MHERILEGQNEMRELMVSNYLPWYLRKGVLIFFSVVLLALLIAVVSFFIITGVVVHS", "aa_hexdigest": "1b2f91b1dccc1b0c699623e34bd32845", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6592.876599999998, "isoelectric_point": 6.70656909942627 }, "id": "GIHJHMOALN_563", "locus": "GCA009939745_00528" }, { "type": "cds", "sequence": "contig_1", "start": 298813, "stop": 299343, "strand": "+", "frame": 1, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872", "KEGG:K06949", "SO:0001217", "UniRef:UniRef50_P75523", "UniRef:UniRef90_P75523" ], "nt": "TTGTTTAAATTGTTAATCCATTTTCACGCTCAAAACGTGGCACGGTTAGTGTTAGTCATTACTAAAAACGACCTTGAGTTTGAACCTGCTGAAAAAGCCCGTTTAAGTGAACTAACCAGCTTTGGTTACCAACTCTTTTTTACAGCCCCTAATGCTGATTTACCTCTCACTTTATTTACAGAATTGCAAAACCGTTTTAGTGTATTCATGGGACAATCAGGGGTCGGTAAGTCTAGCCTTATTAACCGTTTGGACAGTCAAATTCACCAAGCGATCCAAGCGCTTTCAGCACACCAGTTCGGTAAAAACACCACCACTTCCACGGTGATGTTTCCCTTCCAAAACGGCTTTATTTGTGACACCCCTGGCTTTAATGTAATTGACTTTCCCAACCTCAAACAATTAGCAGCCCAACACTTTGTCGGTTTTGCTAGCTTAATTGGTCAATGCCATTTTTCCAACTGCACCCATCAAAGCGAAAAAGGTTGTTTTATTGTAAGTGCTGTAACCCAAAAATCGTATTCCTTGTGA", "aa": "MFKLLIHFHAQNVARLVLVITKNDLEFEPAEKARLSELTSFGYQLFFTAPNADLPLTLFTELQNRFSVFMGQSGVGKSSLINRLDSQIHQAIQALSAHQFGKNTTTSTVMFPFQNGFICDTPGFNVIDFPNLKQLAAQHFVGFASLIGQCHFSNCTHQSEKGCFIVSAVTQKSYSL", "aa_hexdigest": "8fb81df9a2babc17ea5d87eaf5e50f5f", "start_type": "TTG", "rbs_motif": null, "genes": [ "rsgA" ], "truncated": "both", "pseudogene": { "start": 298540, "stop": 299376, "observations": { "mutation": [ 298645, 298804, 299341 ], "stop": [ 298645, 298804, 299341 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75523", "query_cov": 1.0, "subject_cov": 0.6330935251798561, "identity": 0.9940000000000001, "score": 352.0, "evalue": 2.33e-121, "gene_start": 1, "gene_end": 176, "reference_start": 92, "reference_end": 267, "reference_sequence": "MSIRKTGIVFQRFGKQFKVFVENQTLVAFAQKKLQWKRDFKLLVGDKVTLENGVIVAVEERKNTLVRPKVVNVDQVVIVQSLIEPKINWQQLFKLLIHFHAQNVARLVLVITKNDLEFEPAEKARLSELTSFGYQLFFTAPNADLPLTLFTELQNRFSVFMGQSGVGKSSLINRLDSQIHQAIQALSAHQFGKNTTTSTVMFPFQNGFICDTPGFNVIDFPNLKQLAAQHFVGFASLIGQCHFSNCTHQSEKGCFIVSAVTQKSYSLWLYESYLKLIN" }, "paralog": false, "identity": 0.9856115107913669, "score": 535.0, "evalue": 7.76e-196, "description": "Internal stop codon at 298645, 298804, 299341. Nonsense mutation around 298645, 298804, 299341.", "psc": { "uniref90_id": "UniRef90_P75523", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.6.1.-" ], "uniref50_id": "UniRef50_P75523", "cog_id": "COG1162", "cog_category": "J", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75523", "EC:3.6.1.-", "UniRef:UniRef50_P75523", "COG:COG1162", "COG:J", "KEGG:K06949", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75523" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" } }, "id": "GIHJHMOALN_564", "locus": "GCA009939745_00529" }, { "type": "cds", "sequence": "contig_1", "start": 299398, "stop": 299790, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00342.24" ], "nt": "ATGGAAAGTAAATGGTTAACAGTTGACACTAAGCACCTCTATGGTTTTGATGAAGCGATTTTTATTCAGAAATACCAAAAAAAAGTTAACCAAATTCACCAACAGTTTTTAAATCAACAACTACCCGATGGTCACATGAATGGCTGGTATAGTCAACCCGATCAAGACCACAAGGGTTTGCTAAAACAAATTAACACAATTGCCAAACAGTTTAATGCTCTAAAAGTAACTGACATAGTGTATTTAGGTATTGGTGGTTCTTATACTGGCATTCGTGCCATCTTAGACTTTTTAAAACCAGAACAGAAGGCGAATATCAAAGTGCACTTTGTTCCTGACATATCGGCCTTTAACATTGCTGCAGTTGCTAAAGCAATTAAGGGTAAATCATGA", "aa": "MESKWLTVDTKHLYGFDEAIFIQKYQKKVNQIHQQFLNQQLPDGHMNGWYSQPDQDHKGLLKQINTIAKQFNALKVTDIVYLGIGGSYTGIRAILDFLKPEQKANIKVHFVPDISAFNIAAVAKAIKGKS", "aa_hexdigest": "093b57f5ee34d0a00a2c8b155210631a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00342.24", "name": "PGI", "length": 55, "aa_cov": 0.4230769230769231, "hmm_cov": 0.9818181818181818, "evalue": 4.998243633592616e-07, "score": 24.126863479614258, "start": 73, "stop": 127 } ], "seq_stats": { "molecular_weight": 14757.835499999981, "isoelectric_point": 9.398410606384278 }, "id": "GIHJHMOALN_565", "locus": "GCA009939745_00530" }, { "type": "cds", "sequence": "contig_1", "start": 299794, "stop": 300153, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAGTAACTTCTAAATCTGGTCGCACACTCGAGCCGGCTGTTACCTTTCGGTACTTCCGTAATTTATTGCACAAACAATACAAGCAAAAACATGCTTTACGCACAGTTGTGATTACCGATGCCGTTAAGGGTTTACTAGTTGGCATGAGTAACCAGTACGGTTATGCACACTTAACTATTCCAAGCAATATTGGTGGGCGTTTTTCAACTCTTTCACCCGCCGGTTTACTGTTAGCTAAACTTTGTGGTCATGATCCGAAACAGCTCTTATTGGGTACTTTGACAGCCAAACAAGAGTTAGCAAACAGTGATTTAAACACTAATTCGGCTTATTATTATGCTGCTTTGCGTCACTGA", "aa": "MVVTSKSGRTLEPAVTFRYFRNLLHKQYKQKHALRTVVITDAVKGLLVGMSNQYGYAHLTIPSNIGGRFSTLSPAGLLLAKLCGHDPKQLLLGTLTAKQELANSDLNTNSAYYYAALRH", "aa_hexdigest": "41cde99de6a42c799c7e91b033a01f86", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13119.045899999983, "isoelectric_point": 9.947874641418458 }, "id": "GIHJHMOALN_566", "locus": "GCA009939745_00531" }, { "type": "cds", "sequence": "contig_1", "start": 300184, "stop": 300690, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACGGTAGCATACCACAGTGCTTATGAGTACCTCTTGTTGCAACACCGCCAACTGTTCGGTGAATCAGAGGGTAAAGGTGGTAAGTCCCTGTTCCCTACCTTTTCGCTATTTACCACTGACTTACACTCAATGGGGCAGTTGTACCAAGAAGGGGAAAAGAACTTCTTTGAAACAGTGATACAAGTGCAAACACAATTTCACGACTTGGAACTACCACCATCAGACTTTAACAATGATGATCAGCTAGATTACTTATTGGCTAAAAGTATGAATGAAATTTCAAACACTGCCCTAGAAGCAGTTGTGGAAGCTCATTTTCAATCAAACGTTAACATCATTAAGTTAACGCTCAAAGAAAGAACAACCTTTATGTTTGGTTACTTTTACTTCTGGTTATCAATGGCCACAATGATGAGTGGTTCACTGCTGGGACACAACGTCTTTGATCAACCAGGGGTGGAAGTGTACAAGCAGTTAATGTTTGCTAAACTTGGTCGTGAATAG", "aa": "MTVAYHSAYEYLLLQHRQLFGESEGKGGKSLFPTFSLFTTDLHSMGQLYQEGEKNFFETVIQVQTQFHDLELPPSDFNNDDQLDYLLAKSMNEISNTALEAVVEAHFQSNVNIIKLTLKERTTFMFGYFYFWLSMATMMSGSLLGHNVFDQPGVEVYKQLMFAKLGRE", "aa_hexdigest": "966487e1881d2146614e255c06480633", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19352.730699999975, "isoelectric_point": 5.042266654968262 }, "id": "GIHJHMOALN_567", "locus": "GCA009939745_00532" }, { "type": "cds", "sequence": "contig_1", "start": 300683, "stop": 301315, "strand": "+", "frame": 2, "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "EC:5.1.3.1", "GO:0004750", "GO:0006098", "GO:0019323", "GO:0046872", "SO:0001217", "UniParc:UPI0001DD64FF", "UniRef:UniRef100_A0AB38W7C5", "UniRef:UniRef50_P47358", "UniRef:UniRef90_P75522" ], "nt": "GTGAATAGGGAAATTGCCTTTTCGCTTTTACCACTGTTACACCAGTTTGACCGCAAGTTGTTAGAACAATTCTTTGCGGACGGCTTGCGCTTAATCCACTATGACGTGATGGACCATTTTGTGGACAACACTGTCTTTCAAGGGGAACATTTAGATGAATTACAACAAATTGGTTTTCAGGTCAATGTACATTTGATGGTACAAGCATTAGAACAAATCTTGCCAGTATACCTCCACCATCAAGCCGTTAAGCGTATTTCCTTTCACGTTGAACCGTTTGATATCCCTACCATCAAACACTTTATTGCGCAAATTAAACAAGCGGGAAAACAAGTCGGTTTAGCGTTTAAATTCACCACTCCGCTTGTAAATTACGAGCGCTTGGTGCAACAACTGGACTTTGTTACCTTAATGAGTGTCCCCCCAGGAAAGGGCGGGCAAGCCTTTAACAGTGCTGTTTTTAATAACTTAAAACAAGCCCACAAATACCACTGCTCAATTGAAATCGATGGTGGGATCAAATTAGATAACATTCACCAAATCCAGGATGATGTCAATTTTATTGTCATGGGAAGTGGCTTTATAAAATTAGAACGATGGCAGCGCCAACAGCTGCTCAAAACCAATCAATAA", "aa": "MNREIAFSLLPLLHQFDRKLLEQFFADGLRLIHYDVMDHFVDNTVFQGEHLDELQQIGFQVNVHLMVQALEQILPVYLHHQAVKRISFHVEPFDIPTIKHFIAQIKQAGKQVGLAFKFTTPLVNYERLVQQLDFVTLMSVPPGKGGQAFNSAVFNNLKQAHKYHCSIEIDGGIKLDNIHQIQDDVNFIVMGSGFIKLERWQRQQLLKTNQ", "aa_hexdigest": "179887d09df0e062c70b45ea8ae49ba4", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64FF", "uniref100_id": "UniRef100_A0AB38W7C5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64FF", "UniRef:UniRef100_A0AB38W7C5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7C5", "uniref90_id": "UniRef90_P75522" }, "psc": { "uniref90_id": "UniRef90_P75522", "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "ec_ids": [ "5.1.3.1" ], "uniref50_id": "UniRef50_P47358", "cog_id": "COG0036", "cog_category": "G", "go_ids": [ "GO:0004750", "GO:0046872", "GO:0019323", "GO:0006098" ] }, "pscc": { "uniref50_id": "UniRef50_P47358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47358" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [ "rpe" ], "id": "GIHJHMOALN_568", "locus": "GCA009939745_00533" }, { "type": "cds", "sequence": "contig_1", "start": 301400, "stop": 302689, "strand": "+", "frame": 2, "gene": "asnS", "product": "asparagine--tRNA ligase", "db_xrefs": [ "COG:COG0017", "COG:J", "EC:6.1.1.22", "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421", "KEGG:K01893", "SO:0001217", "UniRef:UniRef50_P47359", "UniRef:UniRef90_P75521" ], "nt": "TTGAAAAACAAACGTACTAGCGCCAACATTATCTTTTTGGAAGTTAATGATGGATCAACGCTGTTAAACCTACAAGCGGTGGTTAAACAAGACCAACCGGAGTTGTTTGCATTAGCTGAATCAATTAGTTTAGCGAGTGCTGTAAGTGTTTCTGGTACTGTAGCGTTAACACCCAAATCCAAACAACCCTTGGAATTAGTAGTCAAACAAATTAATGTATTGAGTACTGCGCGAGCAGACTATCCACTCCAAAAAAAGGAACACAGCTTGGAGTTTTTCCGCAACAACGCCTATTTGCGCGTACGAGCCCGTACCTACTTTGCCATCATGAAAGTGCGCAGTCTGTTGTCACAAGCCATTTTTGACTACTTCTTTAAGAATGACTTTGTTTTAGTGCACAGTCCAATTCTTACTAGCAACGACTGTGAGGGGGCAGGTGAGACCTTTGAATTAAAGCAAGGTAAGGAATTCTTTAATAAGACTACTTACCTTACTGTCAGTGGTCAGTTTGGTGCGGAGTGTTACGCCCAAGCCTTTAAAAAAGTGTTTACCTTCGGGCCGACTTTTCGCGCTGAAAAATCACACACTTCACGGCACCTTAGTGAGTTTTGGATGATTGAACCAGAAGTTGCTTTTGCCAACCTCAAGGACTTAATTAAGCTAATTGAAAGCACTGTCAAAACCGTGATTAAACAGGTTATGCAAAAAGCCAAGCAGGAACTCGACTTTTTAGAAAAGCAGTTTGATGTCAAATTAATGGAACGCTTAAAGCAAATTACAAGTACCAAAAACTTCCATGTTTTGGAATACATCAAGGCCTTAGAGATTTTAAAAACAGCCCAAGCTAGTGGTCAAGCTAACTTTGAGGTACAAGACTTTAACTTCGGTTTAGATCTCAAAACGGAACACGAACGCTTTTTGTGTGAACAGCATTTTCACAACCAACCGGTGTTTGTAATTAACTACCCCAAGGACCTTAAGGCCTTTTACATGAAGCAAAATGCTGATGGTAGAACTGTTGGTGCGGTTGACCTTTTATTTCCCCAAATTGGTGAGATCTGTGGTGGGAGTGAACGGGAAGGTAATCTAGAAAAACTAGTGGAACGTTGCCAAGCAATGCAAATTGATACCCAAACACTTAACTGGTATTTAGATATGCGTAAATGGGGTTACTTTGCCTCAGCTGGCTTTGGTTTAGGGTTTGATCGTTTACTAGCTTACATCTGTGGATTGGAAAACATCCGTGATGCGATCCCCTTTCCCCGTGCCCATGGTTCCATTAACTATTAA", "aa": "MKNKRTSANIIFLEVNDGSTLLNLQAVVKQDQPELFALAESISLASAVSVSGTVALTPKSKQPLELVVKQINVLSTARADYPLQKKEHSLEFFRNNAYLRVRARTYFAIMKVRSLLSQAIFDYFFKNDFVLVHSPILTSNDCEGAGETFELKQGKEFFNKTTYLTVSGQFGAECYAQAFKKVFTFGPTFRAEKSHTSRHLSEFWMIEPEVAFANLKDLIKLIESTVKTVIKQVMQKAKQELDFLEKQFDVKLMERLKQITSTKNFHVLEYIKALEILKTAQASGQANFEVQDFNFGLDLKTEHERFLCEQHFHNQPVFVINYPKDLKAFYMKQNADGRTVGAVDLLFPQIGEICGGSEREGNLEKLVERCQAMQIDTQTLNWYLDMRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRAHGSINY", "aa_hexdigest": "c5a00f7bd214d3f146213dfe2a3f1015", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75521", "query_cov": 1.0, "subject_cov": 0.9428571428571428, "identity": 0.993, "score": 842.0, "evalue": 9.91e-308, "valid": true, "gene": "asnS", "product": "asparagine--tRNA ligase", "ec_ids": [ "6.1.1.22" ], "uniref50_id": "UniRef50_P47359", "cog_id": "COG0017", "cog_category": "J", "kegg_orthology_id": "K01893", "go_ids": [ "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421" ] }, "pscc": { "uniref50_id": "UniRef50_P47359", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47359" ], "product": "Asparagine--tRNA ligase" }, "genes": [ "asnS" ], "id": "GIHJHMOALN_569", "locus": "GCA009939745_00534" }, { "type": "cds", "sequence": "contig_1", "start": 302964, "stop": 303122, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTATTAACGGTGTCTTAATTGTCCTAGTAGCATATGGCTACTTTCACTTTAGTTTTTTAATTGTGATTGTGCTGCGTGATTTAGTGCTAGACGGATTGCGCTTTTACGCTCAGGAAAAACAGCTAATTATTCCCGCCAACCAATGGGGTAAGTGA", "aa": "MIINGVLIVLVAYGYFHFSFLIVIVLRDLVLDGLRFYAQEKQLIIPANQWGK", "aa_hexdigest": "54e7496a4971a6b611a7e82135b38c35", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6009.154299999999, "isoelectric_point": 8.16035976409912 }, "id": "GIHJHMOALN_570", "locus": "GCA009939745_00535" }, { "type": "cds", "sequence": "contig_1", "start": 303228, "stop": 303329, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATTTGCCCTATTATTTAGCTACAGCCTTCTCCTTGGTCTCGTTTGGTATTTACGCCCAACAAATATATAAAACCATTAAGGTTAAAGTAAAGTTATAA", "aa": "MHLPYYLATAFSLVSFGIYAQQIYKTIKVKVKL", "aa_hexdigest": "b18b99d24366c3ffd08f935b0d49e79d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3835.5974000000006, "isoelectric_point": 9.778580665588379 }, "id": "GIHJHMOALN_571", "locus": "GCA009939745_00536" }, { "type": "cds", "sequence": "contig_1", "start": 303351, "stop": 303824, "strand": "+", "frame": 3, "gene": "pncC", "product": "Protein MG115-like protein", "db_xrefs": [ "COG:COG1546", "COG:H", "EC:3.5.1.42", "KEGG:K03743", "RefSeq:WP_014325421.1", "SO:0001217", "UniParc:UPI0001DD6502", "UniRef:UniRef100_A0A0H3DKL5", "UniRef:UniRef50_P47361", "UniRef:UniRef90_Q9EXC9" ], "nt": "GTGTACGCGCGCTTAATTGCTGAAAAACTGCTAAATCATAAGCTCACCATTGCCACTGCAGAGTCAGTTACTGGGGGCCTTTTGAGTAGTAGCTTAACCGACATCGCTGGTGCTTCTCGTTTCTTTAAGGGTGCCATTGTGGCATACAGCAATGAATTGAAAAAATCACTTTTAAATGTCAAACAATCAACCTTAATTAACCACGGTGCTGTATCGCGTTATTGTGTTCGTGAAATGGCGTTAGGTTTAATGCAAAAATTCAATGTTGATATTGCCGTGGCCTGCAGTGGGTTAGCGGGTCCTGATGCATTGGAAAACCAAGCAGTTGGTAGCTTGTTTTTCTGTGTGATTGTTGCCAACAAGGCTTATGACTTTGAAACAAAACTACCAGCTGGCTCTAGAAACGAATTGCGCCAGCTTTTTGTGCAAAAAATCTTGCAAACAGTTGAACACATTTTAAGTGAAATTTCTTAG", "aa": "MYARLIAEKLLNHKLTIATAESVTGGLLSSSLTDIAGASRFFKGAIVAYSNELKKSLLNVKQSTLINHGAVSRYCVREMALGLMQKFNVDIAVACSGLAGPDALENQAVGSLFFCVIVANKAYDFETKLPAGSRNELRQLFVQKILQTVEHILSEIS", "aa_hexdigest": "ff2963340c8573ce9368a56cf29ef3ea", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6502", "ncbi_nrp_id": "WP_014325421.1", "uniref100_id": "UniRef100_A0A0H3DKL5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6502", "RefSeq:WP_014325421.1", "UniRef:UniRef100_A0A0H3DKL5" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DKL5", "uniref90_id": "UniRef90_Q9EXC9" }, "psc": { "uniref90_id": "UniRef90_Q9EXC9", "gene": "PncC", "product": "Protein MG115 homolog", "ec_ids": [ "3.5.1.42" ], "uniref50_id": "UniRef50_P47361", "cog_id": "COG1546", "cog_category": "H", "kegg_orthology_id": "K03743" }, "pscc": { "uniref50_id": "UniRef50_P47361", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47361" ], "product": "Protein MG115" }, "genes": [ "pncC" ], "id": "GIHJHMOALN_572", "locus": "GCA009939745_00537" }, { "type": "cds", "sequence": "contig_1", "start": 303850, "stop": 304605, "strand": "+", "frame": 1, "gene": "ispD", "product": "putative MG116-like protein", "db_xrefs": [ "COG:COG1211", "COG:I", "RefSeq:WP_010874612.1", "SO:0001217", "UniParc:UPI00001392BE", "UniRef:UniRef100_P75519", "UniRef:UniRef50_P75519", "UniRef:UniRef90_P75519" ], "nt": "ATGCAGCGTACTTTAAATGTTGGGGTGATCCTGTGTGAAAGCTTTATTTCCAACGCCCAAAACCCAGTTAACTCCTACATTAAGATTTACGAAAACGTGAGGATGTTTGAGTTTGCGATTAAGCTACTTCAAGAATCGCGAATTAACTTCCAAAAGATCCTTTTATACGTTTTGGAAGAGCAAATTCCACTTGTGGAAAAAGTAGTTGCCAAGTACGAAAACTGCTGGGTCTTTCGTTCCAAGCACAACGAGGTTGAGGACATTTATGAAGCCAAGGGCTTTATTGAAGACAAGTACAAAATTGGTTTAAAAAGTTCTAAAAACCAAGCCTCTTCGTATTATGACAACTGTTGTTTTGTTGTCTTGGAAGCATGTCGACCTTTAACTTCCAAGAAGGTTGTTAAAAACGTGTATGAAAAGGCAATGATTGACGGTGCCGCTGTAGCTGTACTACCGTTTGAAAGGCAGCTTGTTTGTGGTGATAATACTAAGGCGGTGCGCCAGTTAAAGGACAAGGGCAACATGAACTATTGGCGTCAAAGTAGTACTTGGGAGTTGCAATTTCCCCAGGCTTATACGCTCAATAAGTTAAACCAGTATCATAAAAACCTCTTTATGAAGGCGCGGAACATGCTTGATTTAATGAACATTTCCGCTAAGAATCCCCTAAGCATTGTCGATGGTAGTGCTTACAGCTTTCGTGTAGTTACTAGCCTGGACTTTGAAATTTTGTTAGGTATTTTGCGCAATGGATAG", "aa": "MQRTLNVGVILCESFISNAQNPVNSYIKIYENVRMFEFAIKLLQESRINFQKILLYVLEEQIPLVEKVVAKYENCWVFRSKHNEVEDIYEAKGFIEDKYKIGLKSSKNQASSYYDNCCFVVLEACRPLTSKKVVKNVYEKAMIDGAAVAVLPFERQLVCGDNTKAVRQLKDKGNMNYWRQSSTWELQFPQAYTLNKLNQYHKNLFMKARNMLDLMNISAKNPLSIVDGSAYSFRVVTSLDFEILLGILRNG", "aa_hexdigest": "ffac60a049bc33a21b2f7b4654c78aad", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001392BE", "ncbi_nrp_id": "WP_010874612.1", "uniref100_id": "UniRef100_P75519", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001392BE", "RefSeq:WP_010874612.1", "UniRef:UniRef100_P75519" ] }, "ips": { "uniref100_id": "UniRef100_P75519", "uniref90_id": "UniRef90_P75519" }, "psc": { "uniref90_id": "UniRef90_P75519", "gene": "IspD", "product": "Uncharacterized protein MG116 homolog", "uniref50_id": "UniRef50_P75519", "cog_id": "COG1211", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_P75519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75519" ], "product": "Uncharacterized protein MG116 homolog" }, "genes": [ "ispD" ], "id": "GIHJHMOALN_573", "locus": "GCA009939745_00538" }, { "type": "cds", "sequence": "contig_1", "start": 304598, "stop": 304822, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGAAAAATAGTTCAACTAATTCATAACTTTAGCGGTACAGAAAAACTAAAAGCAGCTGTTTTTTCACACGACGAACAGTGTTTCTTTGACTTTGTTAGCTTCGATGAATTAAACAATAAGCTAACTGGGTTTCTGTTATTTGACAGCTTGGAGAAGCTGTTCAAACTAGTAGAAACAATTCAGCAAAAGCGCAGTTGGCTTTATGTTGATGAATTGTGA", "aa": "MDRKIVQLIHNFSGTEKLKAAVFSHDEQCFFDFVSFDELNNKLTGFLLFDSLEKLFKLVETIQQKRSWLYVDEL", "aa_hexdigest": "c5102e24bef098b293a4c1a3c04ac7d6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8805.008599999994, "isoelectric_point": 5.2073270797729485 }, "id": "GIHJHMOALN_574", "locus": "GCA009939745_00539" }, { "type": "cds", "sequence": "contig_1", "start": 304877, "stop": 305257, "strand": "+", "frame": 2, "gene": null, "product": "MPN256 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75518", "UniRef:UniRef90_P75518" ], "nt": "GTGAAAAAGGAGCTTGCGCAAGTCGGTGTTTTAACCCAATTGGATACTAGTTTAGTCAAGCTTTTAATGGCTTCCAAAAATATTGACAGTGCCCTTTACAACACTTACATTAAACCAGTGGAGTTGCAACAGTTTACCCAAACACCAGCTCCTGATAACGTTAATGCAGAGCAATCGCATTTAACCTTGGAGTCTACCACCGACTTGAACAACAGTCAACTAGCCAATACTCCAGCGTTGTGGGAAGTGGAACAAACGACCCAGGAATTGTTACCAACCATGGACTTTTCTAAGTTTATTGATGAATTAGATCAAATTACCAAAAACTTTTCTGATTTAGAGTTAGAACCACTTAGTTTTAACGAAGGTTTTGATGAGTGA", "aa": "MKKELAQVGVLTQLDTSLVKLLMASKNIDSALYNTYIKPVELQQFTQTPAPDNVNAEQSHLTLESTTDLNNSQLANTPALWEVEQTTQELLPTMDFSKFIDELDQITKNFSDLELEPLSFNEGFDE", "aa_hexdigest": "7923285ce1811759232f8d4ae91412a2", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 304598, "stop": 305269, "observations": { "mutation": [ 304820, 305255 ], "stop": [ 304820, 305255 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75518", "query_cov": 0.9920634920634921, "subject_cov": 0.5605381165919282, "identity": 0.992, "score": 234.0, "evalue": 1.24e-76, "gene_start": 2, "gene_end": 126, "reference_start": 95, "reference_end": 219, "reference_sequence": "MDRKIVQLIHNFSGTEKLKAAVFSHDEQCFFDFVSFDELNSKLTGFLLFDSLEKLFKLVETIQQKRSWLYVDELWLINTATENQQLNEVSVWLVKKELAQVGVLTQLDTSLVKLLMASKNTDSALYNTYIKPVELQQFTQTPAPDNVNAEQSHLTLESTTDLNNSQLANTPALWEVEQTTQELLPTMDFSKFIDELDQITKNFSDLELEPLSFNEGFDEWNQE" }, "paralog": false, "identity": 0.9775784753363229, "score": 429.0, "evalue": 1.13e-155, "description": "Internal stop codon at 304820, 305255. Nonsense mutation around 304820, 305255.", "psc": { "uniref90_id": "UniRef90_P75518", "product": "MPN256 family protein", "uniref50_id": "UniRef50_P75518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75518", "UniRef:UniRef50_P75518" ] }, "pscc": { "uniref50_id": "UniRef50_P75518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75518" ], "product": "Uncharacterized protein MG117 homolog" } }, "id": "GIHJHMOALN_575", "locus": "GCA009939745_00540" }, { "type": "cds", "sequence": "contig_1", "start": 305250, "stop": 305987, "strand": "+", "frame": 3, "gene": null, "product": "NAD-dependent epimerase/dehydratase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D" ], "nt": "ATGAGTGAAACCAAGAGTAAAGTACTCGTTTTAGGCGGTTTAGGTTACATTGGGAGCTGTTTTATTGACCAGCTTCTAAAACAGTACCCTGATGTAACTGTTAGTGTAATTGACATTAACCACACTAGTCTGGCGCTACAACTGTTACCAAGACAGGTCAACGTTCACTTTGTTAACTTACTTGACCGTGCACAGTTAACAGATACGATTGCTCAAATTAACCCTGATGTCGTGTTTCACTTTGCGGCAAAAACAAGCGTAAAGGAATCGACCGAACAACCACTTACATATTTTGACCATAACCTAGTCGGTACGCTCAACTTACTCCACGCTTTAAAAGAACTACAAAAACCGATCCAGCTGTTCTTCTCCTCCACTGCTGCAGTCTTTGGTAGTGCTAGTACACTTCCAATTCCAGAAAACTTAGTTTTAGAGGAAACACTCGCTTCCAATCCGTATGGCATTAGTAAGTTTTTAAGTGAAATAGTACTGCAAACACTTACCCGTAGCCCCCATTTTCAAGTAATAGCGTTGCGCTACTTTAACGTAGCGGGTGCTAGTAATCCCTTTGGTAACTTCAATAAAAACACGACCTTACTGATTCCTAACCTGATTAAAGCCTTTATGGAAAAGCGCACCTTCTTTCTGTATGGTGATGACTATGACACTAAAGATGGGAGCTGTATCCGTGATTACATCCATGTAGTAGACTTGTGTGATGCCCATTTGTTGGCGTGA", "aa": "MSETKSKVLVLGGLGYIGSCFIDQLLKQYPDVTVSVIDINHTSLALQLLPRQVNVHFVNLLDRAQLTDTIAQINPDVVFHFAAKTSVKESTEQPLTYFDHNLVGTLNLLHALKELQKPIQLFFSSTAAVFGSASTLPIPENLVLEETLASNPYGISKFLSEIVLQTLTRSPHFQVIALRYFNVAGASNPFGNFNKNTTLLIPNLIKAFMEKRTFFLYGDDYDTKDGSCIRDYIHVVDLCDAHLLA", "aa_hexdigest": "1c4773f5a4ecc519cd36a8a96af491eb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557C9D", "query_cov": 0.8816326530612245, "subject_cov": 0.9432314410480349, "identity": 0.537, "score": 236.0, "evalue": 3.71e-75, "valid": false, "product": "NAD-dependent epimerase/dehydratase family protein", "uniref50_id": "UniRef50_UPI0000557C9D" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557C9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D" ], "product": "NAD-dependent epimerase/dehydratase family protein" }, "genes": [], "id": "GIHJHMOALN_576", "locus": "GCA009939745_00541" }, { "type": "cds", "sequence": "contig_1", "start": 305994, "stop": 306266, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGCCAATCCTAAAGTGCGCTTTGAAAGCTTTAACTTAGGTAGTGGTCAGGGCTTTTCCAATTGGGAGGTCATTAATACTGCTCAAGCAATCTTTGCGCCTGAACAATTACAGCTCAAAATTGAATCACGAAGGGCTGGTGATCCACCTGTTTTAGTGGTTGATTGCACGAAGGCAAAACGCTTACTCAACTTCCAACCAACCCGCTCATTACACAAAATGCTGAGCGATGAAACAATTTTTTATCGGGATTTTTATAATAGGCTTTAA", "aa": "MQANPKVRFESFNLGSGQGFSNWEVINTAQAIFAPEQLQLKIESRRAGDPPVLVVDCTKAKRLLNFQPTRSLHKMLSDETIFYRDFYNRL", "aa_hexdigest": "bb297810e6c1c35ac8b02d6256f7e693", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10390.765999999992, "isoelectric_point": 9.355990409851074 }, "id": "GIHJHMOALN_577", "locus": "GCA009939745_00542" }, { "type": "cds", "sequence": "contig_1", "start": 306302, "stop": 307225, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030B82AEE" ], "nt": "ATGGAAAATATCTGCAAGAGTTTTGACAATGGTAGGGTCAAAGCTAATGTAGATGTTAACCTCACGGTTTACGAAAATACTGTCCACACATTGCTGGGTGAAAATGGTGCGGGCAAATCTACTTTAACTTCCATTTTGTTCGGTTTATACCAACCCGACAGCGGCAAAATTTTTATTGGTGAAGAGGAAGTACACTTCAAGTCCTCCAAGGATGCCGTGCAGCACAAAATTGGGATGGTGCACCAGCACTTTAAGCTGGTTGATAACTACACCGTCTTAGACAACATTATTTTGGGCAATGAAAGTAGTTTTTCCATTCCTTTTACCAACGGTAAGCTTAAACTACCTTTATTGCACCGTAAAGCTAGCGAAGCCAAAATTCAAGCAATGATGGAACGGTATGACCTCCATGTGAACTTGCACCAAAAGGTAAGCCGCTTAACGGTGGGTCAGCAACAACGGGTCGAGATCTTGAAGGTGTTATTCCGTGACAGTGACATTCTCATCTTTGATGAACCAACCGCTGTTTTGAGCGACCAGGAGATCAAGAGCTTTTTAAACATCATTAAAAACTTCAAGAAGATGGGTAAAACGATTGTGCTCATCTCCCACAAACTCAACGAAATTAAGGAAGTAGCGGAGACTGCTACCATTTTGCGTCAAGGTCACTCCGTTGGCACCTTCCAAATTAAGGACACTTCAATTGATGAAATGGCACGCTTAATGATGGGAAAGGAGTTGAAGAAAACCAAAAACAATACTCAGTTTACCGCCAAAGGTGAGCCCGTTTTAAAGGTCGAAAACCTCCATCTTTATTTAAACCAAAACTGGTTTTACAAGTTAATTGCGCGCTGGAACCAAAAGCGAATTAATCAACTCCAAAAACAGGGTAAGCCAGCGAAAACACTGTGGTTAAAGAGTTGA", "aa": "MENICKSFDNGRVKANVDVNLTVYENTVHTLLGENGAGKSTLTSILFGLYQPDSGKIFIGEEEVHFKSSKDAVQHKIGMVHQHFKLVDNYTVLDNIILGNESSFSIPFTNGKLKLPLLHRKASEAKIQAMMERYDLHVNLHQKVSRLTVGQQQRVEILKVLFRDSDILIFDEPTAVLSDQEIKSFLNIIKNFKKMGKTIVLISHKLNEIKEVAETATILRQGHSVGTFQIKDTSIDEMARLMMGKELKKTKNNTQFTAKGEPVLKVENLHLYLNQNWFYKLIARWNQKRINQLQKQGKPAKTLWLKS", "aa_hexdigest": "a8abefd1433abf18a4b202ec900ef133", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030B82AEE", "query_cov": 0.8664495114006515, "subject_cov": 0.9779411764705882, "identity": 0.508, "score": 243.0, "evalue": 1.77e-76, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI0030B82AEE" }, "pscc": { "uniref50_id": "UniRef50_UPI0030B82AEE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030B82AEE" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "GIHJHMOALN_578", "locus": "GCA009939745_00543" }, { "type": "cds", "sequence": "contig_1", "start": 307235, "stop": 307603, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGGCAGCTATTGAAAAAACACCCCGTTTTATTCGTGGAATCGTTAATAATTTAGGTTTTGGTAGCCAACAGGTGTTTGATAAAGGGATTAGTTTTGAGATCCATAAGGGTGAAATTTTTGCCATTGCCGGGGTGGAAGGTAACGGTCAAAACCAATTAATAGATCTTATTTGTGGCTTGGAAAAAGCAGCACCAAAAAAGGTCTTCTTCAATGGTTTTGACATTTCACGTTATTCCATTCGCAAGCGGATTAACGCTGGTATTGGTTTTGTGTTAGAAGACCGCCATAAATACGGATTAATCTTAGACCAAACGGTGCGTTTTAACGCGGTGAATAACCAAATTGATCGTAAGCAATTTAGCAGTTGA", "aa": "MAAIEKTPRFIRGIVNNLGFGSQQVFDKGISFEIHKGEIFAIAGVEGNGQNQLIDLICGLEKAAPKKVFFNGFDISRYSIRKRINAGIGFVLEDRHKYGLILDQTVRFNAVNNQIDRKQFSS", "aa_hexdigest": "ed13da761070c5bc4fd52bc221665a26", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 82, "aa_cov": 0.6721311475409836, "hmm_cov": 0.9512195121951219, "evalue": 3.835653987002905e-09, "score": 32.75098419189453, "start": 28, "stop": 109 } ], "seq_stats": { "molecular_weight": 13676.539799999979, "isoelectric_point": 9.607094764709473 }, "id": "GIHJHMOALN_579", "locus": "GCA009939745_00544" }, { "type": "cds", "sequence": "contig_1", "start": 307619, "stop": 307999, "strand": "+", "frame": 2, "gene": "mglA", "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "nt": "ATGCAAATAGCTGTTTACACTAACCAAATCGTCGATAAATTTGATGTTCGTGGAGCGGTACAAGGTACAGCAATAGTGCGTTTACTTTCCGGGGGTAATCAGCAAAAGCTGATTATTGGTCGGGAACTGACAAAGCAGAACGAGCTGTTAGTGTTTGCTCAAGTAACCCGGGGTTTGGATGTTGGTGCCATTTCGTTTATCCACCAAAAGATTTTGGATGCAAAGAAACAAAATAATGCAATCTTGTTAGTGTCATATGAGTTAGATGAAATTTTAGCTATTGCCGATACCATTGGCGTAATTAACAAAGGCAACCTGTTGGAAGTCAATAAACGTGATGTAATGACGCGTGAACGCATAGGCAAACTAATCATGCAATAA", "aa": "MQIAVYTNQIVDKFDVRGAVQGTAIVRLLSGGNQQKLIIGRELTKQNELLVFAQVTRGLDVGAISFIHQKILDAKKQNNAILLVSYELDEILAIADTIGVINKGNLLEVNKRDVMTRERIGKLIMQ", "aa_hexdigest": "5ac53fec40fc3a7da533f40dc03ef0b4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M965", "query_cov": 0.9206349206349206, "subject_cov": 0.9354838709677419, "identity": 0.5429999999999999, "score": 126.0, "evalue": 4.14e-35, "valid": false, "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "uniref50_id": "UniRef50_A0A223M965" }, "pscc": { "uniref50_id": "UniRef50_A0A223M965", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "product": "Galactose/methyl galactoside import ATP-binding protein MglA" }, "genes": [ "mglA" ], "id": "GIHJHMOALN_580", "locus": "GCA009939745_00545" }, { "type": "cds", "sequence": "contig_1", "start": 308016, "stop": 308672, "strand": "+", "frame": 3, "gene": "nupP", "product": "putative MG120-like protein", "db_xrefs": [ "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857", "SO:0001217", "UniRef:UniRef50_P75515", "UniRef:UniRef90_P75515" ], "nt": "ATGAATGCTTTAAATTACCTCAAACACCGTTTTCTGTTTAGTAAAGACAAGTTTTGGTACGCCCCTTTTAAACAAAAGCAACGTCGGAGCATCTACTCCACCTTTTCGTTGATTGTTCTCTCCTTTATTGTTTCGTTCTTTTTAATAGTCGCCATTCCCGGCATTAAAGGCGGTACCTTCTTAGAAATCTTTACCCGTTTATTTAAAGATAGGGTTAACATCGAAAACTTTGCACGACAAATTGCGATTTACACCCTAGCTGCTTTGGCGTTTAGCTTTTGTATGAGTGTTGGGGTGTTTAACATTGGGATTTCGGGACAAATGATGGCTGGCGCTAATTTTGGTTTCATGATGATCCTCAAGGTTTTCCCCGAGTCATTCCGTCCTGCCTTTGGTGGTCAGATCATTACCATCCTCTTAATGATTCTAGGTAGTGTGACCGTTGCTATGGTGGTAGCTGCTTTAAAGGTCTTCTTTAAGGTTAATGAAGTGGTTAGTGCCATTATGCTCAACTGGGTAATTGTGCTGGTTAGCGCTTATCTAGTGGGTACTTACATTAAACCGGAAAAAACTGATACTTCCCAGTTTTACTCGATTGAACTACCGGATGCCTTTGCGCTGTATAACTTCTCTGATGTTCAACAAAAGTATGGTTGA", "aa": "MNALNYLKHRFLFSKDKFWYAPFKQKQRRSIYSTFSLIVLSFIVSFFLIVAIPGIKGGTFLEIFTRLFKDRVNIENFARQIAIYTLAALAFSFCMSVGVFNIGISGQMMAGANFGFMMILKVFPESFRPAFGGQIITILLMILGSVTVAMVVAALKVFFKVNEVVSAIMLNWVIVLVSAYLVGTYIKPEKTDTSQFYSIELPDAFALYNFSDVQQKYG", "aa_hexdigest": "64070d3705970c83c38547397748ff36", "start_type": "ATG", "rbs_motif": null, "genes": [ "nupP" ], "truncated": "3-prime", "pseudogene": { "start": 308016, "stop": 308975, "observations": { "mutation": [ 308670 ], "stop": [ 308670 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75515", "query_cov": 1.0, "subject_cov": 0.42166344294003866, "identity": 0.995, "score": 412.0, "evalue": 1.06e-140, "gene_start": 1, "gene_end": 218, "reference_start": 1, "reference_end": 218, "reference_sequence": "MNALNYLKHRFLFSKDKFWYAPFKQKQRRSIYSTFSLIVLSFIVSFFLIVAIPGIKGGTFLEIFTRLFKDRINIENFARQIAIYTLAALAFSFCMSVGVFNIGISGQMMAGANFGFMMILKVFPESFRPAFGGQIITILLMILGSVTVAMVVAALKVFFKVNEVVSAIMLNWVIVLVSAYLVGTYIKPEKTDTSQFYSIELPDAFALYNFSDVQQKYGWLTSLVIAIAAAIFIAVLMKFTVFGHKLKSTGLSVTGSQAAGYSVKKYQFLSFVISGILSGLLAAVVYTASFEKQLTFSDVGDFGITSVPITGFDGIAIGLIALNSPARIVIVSTIISFVTIGAKPAGLNAATASLVLGIMMYFAAIYNLMIYIKPWRMIVKLNISKMNEAAYDEFQNEMAANLETLSFQRFLDKQKRKHDKERMVWFDTKRFEEYQKKKQATLQTFHENSSQNLLQYWKQQLLVADVKRLTFKWDFLTFKHQQKYILRWYKGKNKKQTALENEFASLNEAISQKLEEK" }, "paralog": false, "identity": 0.9937304075235109, "score": 610.0, "evalue": 7.03e-221, "description": "Internal stop codon at 308670. Nonsense mutation around 308670.", "psc": { "uniref90_id": "UniRef90_P75515", "gene": "NupP", "product": "Uncharacterized protein MG120 homolog", "uniref50_id": "UniRef50_P75515", "cog_id": "COG4603", "cog_category": "F", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75515", "UniRef:UniRef50_P75515", "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P75515", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75515" ], "product": "Uncharacterized protein MG120 homolog" } }, "id": "GIHJHMOALN_581", "locus": "GCA009939745_00546" }, { "type": "cds", "sequence": "contig_1", "start": 308682, "stop": 309386, "strand": "+", "frame": 3, "gene": "nupP", "product": "putative MG120-like protein", "db_xrefs": [ "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857", "SO:0001217", "UniRef:UniRef50_P75515", "UniRef:UniRef90_P75515" ], "nt": "TTGGTTATTGCCATAGCAGCTGCCATATTCATTGCAGTTTTAATGAAGTTCACCGTTTTTGGACACAAACTCAAGTCCACGGGTTTAAGTGTGACGGGTTCGCAAGCCGCTGGTTACAGTGTTAAGAAGTATCAGTTCCTCTCGTTTGTGATTTCAGGAATCCTTTCTGGTTTACTAGCAGCTGTAGTTTATACCGCATCCTTTGAAAAGCAGTTAACGTTCTCAGATGTGGGTGACTTTGGGATTACATCGGTACCGATTACTGGGTTTGATGGAATTGCAATTGGTTTAATTGCGCTCAACAGTCCAGCGCGAATTGTAATAGTTTCCACCATTATTTCATTTGTAACCATTGGTGCTAAACCAGCGGGGTTAAACGCTGCTACTGCGAGCTTGGTGTTGGGTATCATGATGTATTTCGCTGCCATTTATAACCTGATGATTTACATCAAGCCATGGCGCATGATTGTCAAGCTCAATATTGGTAAGATGAACGAAGCGGCATATGATGAATTCCAAAACGAGATGGCTGCTAATTTAGAAACGCTGTCGTTCCAGCGCTTTTTAGATAAACAAAAACGGAAGCATGATAAAGAGCGAATGGTTTGGTTTGACACCAAGCGCTTTGAGGAGTACCAGAAAAAGAAGCAAGCAACTCTACAAACCTTCCATGAAAATTCCTCGCAAAACTTACTGCAGTACTGA", "aa": "MVIAIAAAIFIAVLMKFTVFGHKLKSTGLSVTGSQAAGYSVKKYQFLSFVISGILSGLLAAVVYTASFEKQLTFSDVGDFGITSVPITGFDGIAIGLIALNSPARIVIVSTIISFVTIGAKPAGLNAATASLVLGIMMYFAAIYNLMIYIKPWRMIVKLNIGKMNEAAYDEFQNEMAANLETLSFQRFLDKQKRKHDKERMVWFDTKRFEEYQKKKQATLQTFHENSSQNLLQY", "aa_hexdigest": "b82c9393085780e9f97cd05add242d8b", "start_type": "TTG", "rbs_motif": null, "genes": [ "nupP" ], "truncated": "both", "pseudogene": { "start": 308382, "stop": 309569, "observations": { "mutation": [ 308670, 309384, 309432 ], "stop": [ 308670, 309384, 309432 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75515", "query_cov": 1.0, "subject_cov": 0.4526112185686654, "identity": 0.991, "score": 430.0, "evalue": 1.52e-147, "gene_start": 1, "gene_end": 234, "reference_start": 223, "reference_end": 456, "reference_sequence": "MNALNYLKHRFLFSKDKFWYAPFKQKQRRSIYSTFSLIVLSFIVSFFLIVAIPGIKGGTFLEIFTRLFKDRINIENFARQIAIYTLAALAFSFCMSVGVFNIGISGQMMAGANFGFMMILKVFPESFRPAFGGQIITILLMILGSVTVAMVVAALKVFFKVNEVVSAIMLNWVIVLVSAYLVGTYIKPEKTDTSQFYSIELPDAFALYNFSDVQQKYGWLTSLVIAIAAAIFIAVLMKFTVFGHKLKSTGLSVTGSQAAGYSVKKYQFLSFVISGILSGLLAAVVYTASFEKQLTFSDVGDFGITSVPITGFDGIAIGLIALNSPARIVIVSTIISFVTIGAKPAGLNAATASLVLGIMMYFAAIYNLMIYIKPWRMIVKLNISKMNEAAYDEFQNEMAANLETLSFQRFLDKQKRKHDKERMVWFDTKRFEEYQKKKQATLQTFHENSSQNLLQYWKQQLLVADVKRLTFKWDFLTFKHQQKYILRWYKGKNKKQTALENEFASLNEAISQKLEEK" }, "paralog": false, "identity": 0.9898734177215189, "score": 750.0, "evalue": 5.66e-276, "description": "Internal stop codon at 308670, 309384, 309432. Nonsense mutation around 308670, 309384, 309432.", "psc": { "uniref90_id": "UniRef90_P75515", "gene": "NupP", "product": "Uncharacterized protein MG120 homolog", "uniref50_id": "UniRef50_P75515", "cog_id": "COG4603", "cog_category": "F", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75515", "UniRef:UniRef50_P75515", "COG:COG4603", "COG:F", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P75515", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75515" ], "product": "Uncharacterized protein MG120 homolog" } }, "id": "GIHJHMOALN_582", "locus": "GCA009939745_00547" }, { "type": "cds", "sequence": "contig_1", "start": 309569, "stop": 309778, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACAGTCTTTAATTTCTTTTAGTAACTTAGATAACTGGTTGTTTGTCGCTCCTGCACTGCTACTCGCTGTATTGAGTGGTTACTTATCTGAGCGGGTTGGTATTGTCAATATTGCCATTAACGGGGGCATGGTGTTTGGCGGGATGTTCTTATCCTTAATGTCTTATGCCTTTGTGCCCAATGCAAATGATTCTGCACCATCTTGA", "aa": "MSQSLISFSNLDNWLFVAPALLLAVLSGYLSERVGIVNIAINGGMVFGGMFLSLMSYAFVPNANDSAPS", "aa_hexdigest": "c9258b06627954a98cba854935a5f624", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7300.407999999999, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_583", "locus": "GCA009939745_00548" }, { "type": "cds", "sequence": "contig_1", "start": 309872, "stop": 310303, "strand": "+", "frame": 2, "gene": "nupQ", "product": "putative MG121-like protein", "db_xrefs": [ "COG:COG1079", "COG:F", "GO:0005886", "GO:0022857", "KEGG:K23536", "SO:0001217", "UniRef:UniRef50_P47367", "UniRef:UniRef90_P75514" ], "nt": "GTGATTGTTGGCACAGGGGTTAACTTACTGGGCACGGGAATTAATTTCTTTGTGGCACAAAATGCCCGCTCATTGCTTAACGATACTGATTTAAGGGTACGATACAGCTTTGTAAGAACAGGAAACTCTGTAAGTATTGAGGGTATAGCAATCTTTGCCTTTGCAATTATCTTTGTTTTATTAGTGTGGTATTTGATGAACTTTACTAAAACGGGCTTGCGTTACCGTGCTGTTGGGGAAAATCCTAATGTGATTGATACCCAAGGTATTAGTGTGTATAAATACCAGTGGTTTGGGGTAATGGCTTCGACGATGGTAGCAGCTTTAGCGGGTTGTTGTTTTGCTTTAAGTCCACAGGTACCTAGCTTTTCTAGCGGGGATGTGAGCGGGTTTGGTTTTATTGCCATTGCCATTATGATCATTTCGATGTGA", "aa": "MIVGTGVNLLGTGINFFVAQNARSLLNDTDLRVRYSFVRTGNSVSIEGIAIFAFAIIFVLLVWYLMNFTKTGLRYRAVGENPNVIDTQGISVYKYQWFGVMASTMVAALAGCCFALSPQVPSFSSGDVSGFGFIAIAIMIISM", "aa_hexdigest": "acee666409cc5e121e0b09cfee537c26", "start_type": "GTG", "rbs_motif": null, "genes": [ "nupQ" ], "truncated": "both", "pseudogene": { "start": 309572, "stop": 310504, "observations": { "mutation": [ 309776, 310301 ], "stop": [ 309776, 310301 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75514", "query_cov": 1.0, "subject_cov": 0.45980707395498394, "identity": 0.993, "score": 278.0, "evalue": 4.11e-92, "gene_start": 1, "gene_end": 143, "reference_start": 102, "reference_end": 244, "reference_sequence": "MSQSLISFSNLDNWLFVAPALLLAVLSGYLSERVGIVNIAINGGMVFGGMFLSLMSYAFVPNANDSAPSWSLAISIPLSVIFASAVGFLFGIAAIKLKADHVIVGTGVNLLGTGINFFVAQNARSLLNDTDLRVRYSFVRTGNSVSIEGIAIFAFAIIFVLLVWYLMNFTKTGLRYRAVGENPNVIDTQGISVYKYQWFGVMASTMVAALAGCCFALSPQVPSFSSGDVSGFGFIAIAIMIISMWRIIPSIVISPLFALAYVLTTGVVGNANNTYLLRTIPFIISLLVMMVFGYLNVGPKNVGKHFDKGLR" }, "paralog": false, "identity": 0.9935483870967742, "score": 594.0, "evalue": 3.54e-219, "description": "Internal stop codon at 309776, 310301. Nonsense mutation around 309776, 310301.", "psc": { "uniref90_id": "UniRef90_P75514", "gene": "NupQ", "product": "Uncharacterized protein MG121 homolog", "uniref50_id": "UniRef50_P47367", "cog_id": "COG1079", "cog_category": "F", "kegg_orthology_id": "K23536", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75514", "UniRef:UniRef50_P47367", "COG:COG1079", "COG:F", "KEGG:K23536", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P47367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47367" ], "product": "Uncharacterized protein MG121" } }, "id": "GIHJHMOALN_584", "locus": "GCA009939745_00549" }, { "type": "cds", "sequence": "contig_1", "start": 310322, "stop": 310504, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTCGCCCTTGTTTGCACTAGCTTATGTGCTTACTACCGGTGTGGTAGGTAATGCTAATAACACTTACTTATTGAGAACTATTCCGTTTATTATTTCGCTGTTGGTAATGATGGTATTTGGATATCTCAATGTAGGGCCGAAAAACGTTGGTAAGCATTTCGATAAGGGTTTGCGATAG", "aa": "MISPLFALAYVLTTGVVGNANNTYLLRTIPFIISLLVMMVFGYLNVGPKNVGKHFDKGLR", "aa_hexdigest": "c3c0b9758f28338dd47dce61416b593b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6599.869200000001, "isoelectric_point": 9.99467868804932 }, "id": "GIHJHMOALN_585", "locus": "GCA009939745_00550" }, { "type": "cds", "sequence": "contig_1", "start": 310541, "stop": 310708, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAAATCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACGTTGCAAAAATATTTACCGAATGACTTTGAAATAGTCTCTACCATTGGTCATATTCGGGAAATGGTGCACAAAAACTTTGGTTTTAACGAAGCCGATTACTCACCAGTTTGGGAAGACTGA", "aa": "MSKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWED", "aa_hexdigest": "233f7614c4ddde2fe0020814a1fc0412", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6409.217500000001, "isoelectric_point": 5.53830051422119 }, "id": "GIHJHMOALN_586", "locus": "GCA009939745_00551" }, { "type": "cds", "sequence": "contig_1", "start": 310892, "stop": 311173, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGAAAAGGACAAGTCGAAGTGCCAACGCATTACCTTTAACGAAATTACCAAGAACGCCGTTTTAGATGCACTGAAAAACCCACGGGAAATTGATCAGAGTTGGGTGCAGAGTCAGTTTGCGCGCCAAATTCTTGACCGGATGATTGGTTTTCGCCTTTCAAGGCTGTTAAACAATTACCTCTCAGCTAAGTCAGCGGGACGGGTACAATCAGTAGCACTGCGTTTTTTAGAAGAACGTGAACAGGAAATTCGTAGCTTTGTACCACGGTTTTGGTGA", "aa": "MDEKDKSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFARQILDRMIGFRLSRLLNNYLSAKSAGRVQSVALRFLEEREQEIRSFVPRFW", "aa_hexdigest": "86088825daf4df9ad4be62be951e9cf5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11062.560499999992, "isoelectric_point": 9.883535194396973 }, "id": "GIHJHMOALN_587", "locus": "GCA009939745_00552" }, { "type": "cds", "sequence": "contig_1", "start": 311183, "stop": 312676, "strand": "+", "frame": 2, "gene": "topA", "product": "type I DNA topoisomerase", "db_xrefs": [ "COG:COG0551", "COG:L", "EC:5.6.2.1", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872", "KEGG:K03168", "SO:0001217", "UniRef:UniRef50_P47368", "UniRef:UniRef90_P78032" ], "nt": "GTGTTGTTAAATCCCAAGGCTGAAGGTGTTAGGGAGGCGTGTGCTAATCGTTCAATTCCAATTGTCTTGCGTGAAATTAATCCTGCTTTACGTGCTGGCTTGAAATTTGAAGAGGAGAAGAGCGTATCGGGAATTGACTTTTTAGATGAAGCATCAGCTAAAAAGTTTGGTGAACAGCTCAAGGGTGTCTTTGAGGTGTACAACATTGATGAAACCAAACACTACAGCAGTTCGCCCAATTCCGCTTACACCACCGCTTCATTGCAAAAGGATGCCATCAACAAACTAGGTTGGTCTTCCAAAAAGGTAACCTTAATTGCCCAACACCTGTATGAAGGGGTGAGTATTAATGGAGAACAAACCGCTTTAATTAGCTATCCCCGTACCGACTCAACTCGTTTATCAGCGCAATTTCAACAAAGTTGTAAAGAATACATCTTAAACCATTACGGAGAAAAGTACCTCTCTAATCGGATTGTAAGCGCTAAGGGAAAAAAAGGTGAAAAAATTATCCAAGACGCCCATGAAGCAATTCACCCAACTGACATGAACATTACCCCGGAAATGGTCAAGAATGCGATTAAGAAGGATGAGTTCTTACTGTACCGCTTAATCTGGATCCGTACGGTCGCTAGTTTAATGGCGGATTGTAAAAAATCACATACCCACATCCGGTTTATTAACGATGGTAATAAGTTTTACGCTTCTTCCAAGTCATTAGTATTTGATGGTTATCGCAAAATTTACGAACACTTTGAAAATAAGGAAAGTAATGATCTCTACATTGATTTGGATAAGATTAGGGTTGGTGATCGTTTCATGGCTAAAGACATTAAGATCACAGCCCGCCAAACCCATCCAGCAGCCCGTTATACCCAAGCTAGTTTAATTGAAGCGTTAGAAAAGTCCAATATTGGTCGACCTTCCACCTATAACACGATGGCTAGTGTTAATTTAGACCGGGGTTATGCTAGTTTAAATAAACATGCTTTTCACGTAACACAACTAGGGGAGCAGGTTAATGAAGAATTGTCCAAACACTTTGGTAAGATCATTAACAAAGAGTTCACTAAAAACATGGAGAAGTCTTTGGATGAAATTGCTGAAAATAAAAAGAACTACCAGGAGTTTCTCCGTGATTTTTGGTCAAACTTTAAGGAAGAGGTAAAGCTAGCGGAGGGTTCGATTCAAAGAGTGAAAAAGGAAAAGGAATTTGTTGGTCGTGATTGTCCAAGCTGTGCTAGTCCCTTGTTGTACCGCTATACTAAACGGGGTAATGAAAAGTTTGTTGGGTGCAGTAAATTTCCCAACTGTAAATACAACGAGTTTAGCCAAAATAAACCGAATTTAACTTTGGAAAAGCTCGAAGAACTGTGTCCGGAGTGTAACAGCCAACTAGTGAAAAGACGCACTAAGTTTAATCCCAATAAAACCTTTGTTGGTTGTAGTAATTTCCCTAGGTGCCGCTACATAAAGAAGGATAATGCTAGCTAG", "aa": "MLLNPKAEGVREACANRSIPIVLREINPALRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQKDAINKLGWSSKKVTLIAQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNHYGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDMNITPEMVKNAIKKDEFLLYRLIWIRTVASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDKIRVGDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKHAFHVTQLGEQVNEELSKHFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEVKLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGNEKFVGCSKFPNCKYNEFSQNKPNLTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS", "aa_hexdigest": "339721df32ded96fd33d4771316c0d48", "start_type": "GTG", "rbs_motif": null, "genes": [ "topA" ], "truncated": "5-prime", "pseudogene": { "start": 310883, "stop": 312676, "observations": { "mutation": [ 311171 ], "stop": [ 311171 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78032", "query_cov": 1.0, "subject_cov": 0.69901547116737, "identity": 0.9940000000000001, "score": 931.0, "evalue": 0.0, "gene_start": 1, "gene_end": 497, "reference_start": 215, "reference_end": 711, "reference_sequence": "MSKNLVVIESPNKVKTLQKYLPNDFEIVSTIGHIREMVHKNFGFNEADYSPVWEDWTKSKKKFSSLSFKGNLKGKKLLSKYDIIKSIKEKASKATNIYLATDPDREGEAISWHVYDVLDEKDKSKCQRITFNEITKNAVLDALKNPREIDQSWVQSQFARQILDRMIGFRLSRLLNNYLSAKSAGRVQSVALRFLEEREQEIRSFVPRFWWTLDVLLNPKAEGVREACANRSIPIVLREINPALRAGLKFEEEKSVSGIDFLDEASAKKFGEQLKGVFEVYNIDETKHYSSSPNSAYTTASLQKDAINKLGWSSKKVTLIAQHLYEGVSINGEQTALISYPRTDSTRLSAQFQQSCKEYILNHYGEKYLSNRIVSAKGKKGEKIIQDAHEAIHPTDINITPEMVKNAIKKDEFLLYRLIWIRTVASLMADCKKSHTHIRFINDGNKFYASSKSLVFDGYRKIYEHFENKESNDLYIDLDKIRVGDRFMAKDIKITARQTHPAARYTQASLIEALEKSNIGRPSTYNTMASVNLDRGYASLNKHAFHVTQLGEQVNEELSKHFGKIINKEFTKNMEKSLDEIAENKKNYQEFLRDFWSNFKEEVKLAEGSIQRVKKEKEFVGRDCPSCASPLLYRYTKRGNEKFVGCSNFPNCKYNEFSQNKPNLTLEKLEELCPECNSQLVKRRTKFNPNKTFVGCSNFPRCRYIKKDNAS" }, "paralog": false, "identity": 0.9949748743718593, "score": 1128.0, "evalue": 0.0, "description": "Internal stop codon at 311171. Nonsense mutation around 311171.", "psc": { "uniref90_id": "UniRef90_P78032", "gene": "topA", "product": "type I DNA topoisomerase", "ec_ids": [ "5.6.2.1" ], "uniref50_id": "UniRef50_P47368", "cog_id": "COG0551", "cog_category": "L", "kegg_orthology_id": "K03168", "go_ids": [ "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78032", "EC:5.6.2.1", "UniRef:UniRef50_P47368", "COG:COG0551", "COG:L", "KEGG:K03168", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47368", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47368" ], "product": "DNA topoisomerase 1" } }, "id": "GIHJHMOALN_588", "locus": "GCA009939745_00553" }, { "type": "cds", "sequence": "contig_1", "start": 312578, "stop": 312772, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGATAATAAGCCAAATGAGGATACTTAAGATCAAAATGGCACTTACCAACGCTCAAATTATTGAGAAGGAAGTGGCACTTCTGCTCGTTAAACTAGCTAGCATTATCCTTCTTTATGTAGCGGCACCTAGGGAAATTACTACAACCAACAAAGGTTTTATTGGGATTAAACTTAGTGCGTCTTTTCACTAG", "aa": "MVIISQMRILKIKMALTNAQIIEKEVALLLVKLASIILLYVAAPREITTTNKGFIGIKLSASFH", "aa_hexdigest": "c3c5dfc23ed23bc10bd50b17dc902371", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7080.638099999997, "isoelectric_point": 10.118586921691893 }, "id": "GIHJHMOALN_589", "locus": "GCA009939745_00554" }, { "type": "cds", "sequence": "contig_1", "start": 312885, "stop": 313139, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGACTTTTACCAACAGCAGATTAACACTACTGCAACTGAGCTGAAGTGGTTGGATGGTCAGTTTCAGCAAATTGATGAAACCGATAAAAAGAAAGCGCATCAAATTACAATTAGACTAGCGCGGAACCAACTGTTACAACAGCTTAGTGTAAAGTTAGACCAAAAGCAGTTTAGTCAACGCGCTAATAATGAACTGCAAAAGTTAAAGTTGTCTAACTTAGAGAGCTTTACTGACCAAAAGATTAAATGA", "aa": "MLDFYQQQINTTATELKWLDGQFQQIDETDKKKAHQITIRLARNQLLQQLSVKLDQKQFSQRANNELQKLKLSNLESFTDQKIK", "aa_hexdigest": "1c9032a66d703ffe3a30586fcb0ff01c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9993.246099999993, "isoelectric_point": 9.346320152282715 }, "id": "GIHJHMOALN_590", "locus": "GCA009939745_00555" }, { "type": "cds", "sequence": "contig_1", "start": 313317, "stop": 313616, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGACTGCAAGCAAAAAACCTATTTAATAAGTTAATTGCTCAAAGTAGCAGTGTCAAGATGAGCGAAAAACTGATTAACAATGCTTTGCAATTAGTTCAGGACAATTTGGCATTACAAGCGAGTGAAAGTGGCAATAAGCTGCTCAAGGAGTTTGAATTATCGTGCACTAACACACAGCTAGTCCAACTGTTGGGCTTTCAGCAGTTTTACTTCGGTACAAACCTGCTTAGCTTACTTGATTTGAGCCGTTCAATCGCTGTTTTAGTGCGCTTTTTAAATGAGCACTGTAAGTGA", "aa": "MKRLQAKNLFNKLIAQSSSVKMSEKLINNALQLVQDNLALQASESGNKLLKEFELSCTNTQLVQLLGFQQFYFGTNLLSLLDLSRSIAVLVRFLNEHCK", "aa_hexdigest": "3b3dc50dad6cd8181041ef113159253d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11202.95829999999, "isoelectric_point": 9.342516517639162 }, "id": "GIHJHMOALN_591", "locus": "GCA009939745_00556" }, { "type": "cds", "sequence": "contig_1", "start": 313638, "stop": 314093, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAACTGCTTTGTTTAACAACTTGCAATGGGTTAATAACAACGACTTCTTTTTAAAGTCACACAATGATTTAAAACAGCTGCACCTCTCCGCTGAACAGTTGGCGATTATTGAACAGCAAAACCGTCCCTTTTACATTGATGCTTATGCTTTATTGATTGCTGGCGTAAAGCAAATGCTGATGGAGCACGATGCTGTGGAACCAAAACAAATTCACTTTCATAATGCCAAAAAGGTAATGGAAAGCTTCCAGCTGTTTGGCATTGACCAGTTAGCGTTAATAGAGTACAACAACTGTCTCTATGGCTTTGTCACCACTAAACTCTATGAGATTAAACAATTAGATGATTTAGCTTTATTTAAAGTTTTGTTTAAAAGCTTTTTGAACAAGCATTTGAAACAAAAATTTGCAACAATCAGTTTATTTGTAAATACCCAAACACTCATGATCTAA", "aa": "METALFNNLQWVNNNDFFLKSHNDLKQLHLSAEQLAIIEQQNRPFYIDAYALLIAGVKQMLMEHDAVEPKQIHFHNAKKVMESFQLFGIDQLALIEYNNCLYGFVTTKLYEIKQLDDLALFKVLFKSFLNKHLKQKFATISLFVNTQTLMI", "aa_hexdigest": "2a1997b59ce9fb766075ff61232ee127", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17700.409200000002, "isoelectric_point": 6.579590797424316 }, "id": "GIHJHMOALN_592", "locus": "GCA009939745_00557" }, { "type": "cds", "sequence": "contig_1", "start": 314297, "stop": 314467, "strand": "+", "frame": 2, "gene": "trxA", "product": "thioredoxin", "db_xrefs": [ "COG:COG3118", "COG:O", "GO:0005737", "GO:0015035", "SO:0001217", "UniRef:UniRef50_P47370", "UniRef:UniRef90_P75512" ], "nt": "GTGAGCACTGTAGCGTTTGCCAAGGTAAATGTCGATGAACAAACTGACATTGCCGCTGCTTACAAAATTACCTCACTACCTACAATTGTTTTATTTGAAAAAGGCCAGGAAAAGCACCGTGCTATTGGTTTTATGCCTAAAGCCAAGATCGTGCAACTTGTCAGCCAATAA", "aa": "MSTVAFAKVNVDEQTDIAAAYKITSLPTIVLFEKGQEKHRAIGFMPKAKIVQLVSQ", "aa_hexdigest": "96c9b5bd4ff0c3b29b76367e283794c2", "start_type": "GTG", "rbs_motif": null, "genes": [ "trxA" ], "truncated": "5-prime", "pseudogene": { "start": 314159, "stop": 314467, "observations": { "mutation": [ 314234 ], "stop": [ 314234 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75512", "query_cov": 1.0, "subject_cov": 0.5490196078431373, "identity": 0.982, "score": 107.0, "evalue": 5.32e-29, "gene_start": 1, "gene_end": 56, "reference_start": 47, "reference_end": 102, "reference_sequence": "MVTEIKSLKQLGELFASNNKVIIDFWAEWCGPCKITGPEFAKAASEVSTVAFAKVNVDEQTDIAAAYKITSLPTIVLFEKGQEKHRAIGFMPKAKIVQLVSQ" }, "paralog": false, "identity": 0.9901960784313726, "score": 198.0, "evalue": 2.28e-67, "description": "Internal stop codon at 314234. Nonsense mutation around 314234.", "psc": { "uniref90_id": "UniRef90_P75512", "gene": "trxA", "product": "thioredoxin", "uniref50_id": "UniRef50_P47370", "cog_id": "COG3118", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0015035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75512", "UniRef:UniRef50_P47370", "COG:COG3118", "COG:O", "GO:0005737", "GO:0015035" ] }, "pscc": { "uniref50_id": "UniRef50_P47370", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47370" ], "product": "Thioredoxin" } }, "id": "GIHJHMOALN_593", "locus": "GCA009939745_00558" }, { "type": "cds", "sequence": "contig_1", "start": 314479, "stop": 314883, "strand": "+", "frame": 1, "gene": "cof", "product": "Putative phosphatase MPN_264", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "SO:0001217", "UniRef:UniRef50_P47371", "UniRef:UniRef90_P75511" ], "nt": "ATGATTGACTTGCTCGGGTTAGATCTTGATGGCACTTTGTTGTCTAGAACAAGGCAAATTAACGATCCTACTAAACAAGCTTTGGCTAATTTAATCCAAAAGAAGCCTAGCTTAAAGGTAATGATTCTAACCGGTAGATCATTATTCTCCACCTTGAAGTACGTTCAAGAGCTCAACGAGCTGTGCAAAAAACCGCTAGTGGAGTACTTTTGCTGTTATGGTGGGGCTAAGTTGTACCAACTCAATAACAACCAACCCCAAGAACAGTACAAGTTTTTAATTGACAGCCGGCAAGTTAAAACAGTGTTTGAAATTGTGGAACAACACAAGGGTTTGTTTTTGGCATACCTCGATAAACCAAAGGCACCCTACATTATTTTGGGGGCAAACCAGTTTTATGCTTGA", "aa": "MIDLLGLDLDGTLLSRTRQINDPTKQALANLIQKKPSLKVMILTGRSLFSTLKYVQELNELCKKPLVEYFCCYGGAKLYQLNNNQPQEQYKFLIDSRQVKTVFEIVEQHKGLFLAYLDKPKAPYIILGANQFYA", "aa_hexdigest": "5e427497861c4538a115bcba94e94620", "start_type": "ATG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 314479, "stop": 315186, "observations": { "mutation": [ 314881 ], "stop": [ 314881 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75511", "query_cov": 1.0, "subject_cov": 0.47686832740213525, "identity": 1.0, "score": 272.0, "evalue": 2.04e-90, "gene_start": 1, "gene_end": 134, "reference_start": 1, "reference_end": 134, "reference_sequence": "MIDLLGLDLDGTLLSRTRQINDPTKQALANLIQKKPSLKVMILTGRSLFSTLKYVQELNELCKKPLVEYFCCYGGAKLYQLNNNQPQEQYKFLIDSRQVKTVFEIVEQHKGLFLAYLDKPKAPYIILGANQFYAWLIKQFWYKQRCEYFKNDHLTDGILKINVYFACPLRLKKVYQIIKRQFQDTLNVVNFSKHLIEITHKDGNKGYAIEAIAKKQGLSLKRMAVIGDSLNDRSMFEKVQYSFAMSKSPDELKLLATEIGTKTNRFRFSSLVDLITEKIIN" }, "paralog": false, "identity": 0.9914893617021276, "score": 468.0, "evalue": 3.73e-170, "description": "Internal stop codon at 314881. Nonsense mutation around 314881.", "psc": { "uniref90_id": "UniRef90_P75511", "gene": "Cof", "product": "Putative phosphatase MPN_264", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P47371", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75511", "EC:3.1.3.-", "UniRef:UniRef50_P47371", "COG:COG0561", "COG:HR", "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P47371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47371" ], "product": "Putative phosphatase MG125" } }, "id": "GIHJHMOALN_594", "locus": "GCA009939745_00559" }, { "type": "cds", "sequence": "contig_1", "start": 314965, "stop": 315324, "strand": "+", "frame": 1, "gene": "cof", "product": "Putative phosphatase MPN_264", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "SO:0001217", "UniRef:UniRef50_P47371", "UniRef:UniRef90_P75511" ], "nt": "GTGTACTTCGCGTGTCCTTTAAGGTTGAAGAAGGTGTACCAGATCATTAAGCGTCAGTTTCAAGACACGCTTAACGTTGTGAGTTTTTCCAAGCACTTAATTGAAATTACCCACAAGGATGGAAATAAGGGCTATGCAATTGAAGCAATTGCCAAAAAACAAGGACTTTCGTTAAAACGGATGGCGGTGATTGGTGATTCCTTAAATGACCGTTCGATGTTTGAAAAGGTACAGTATTCGTTTGCAATGTCGAAATCACCCGATGAACTCAAACTGTTAGCCACTGAAATTGGCACTAAAACAAACCGCTTTCGTTTCAGTAGTTTAGTTGATTTAATTACCGAAAAAATTATTAACTAA", "aa": "MYFACPLRLKKVYQIIKRQFQDTLNVVSFSKHLIEITHKDGNKGYAIEAIAKKQGLSLKRMAVIGDSLNDRSMFEKVQYSFAMSKSPDELKLLATEIGTKTNRFRFSSLVDLITEKIIN", "aa_hexdigest": "69fb60fcf813f16bb097ff835c6cb12a", "start_type": "GTG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "5-prime", "pseudogene": { "start": 314665, "stop": 315324, "observations": { "mutation": [ 314881 ], "stop": [ 314881 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75511", "query_cov": 1.0, "subject_cov": 0.4234875444839858, "identity": 0.983, "score": 231.0, "evalue": 1.96e-74, "gene_start": 1, "gene_end": 119, "reference_start": 163, "reference_end": 281, "reference_sequence": "MIDLLGLDLDGTLLSRTRQINDPTKQALANLIQKKPSLKVMILTGRSLFSTLKYVQELNELCKKPLVEYFCCYGGAKLYQLNNNQPQEQYKFLIDSRQVKTVFEIVEQHKGLFLAYLDKPKAPYIILGANQFYAWLIKQFWYKQRCEYFKNDHLTDGILKINVYFACPLRLKKVYQIIKRQFQDTLNVVNFSKHLIEITHKDGNKGYAIEAIAKKQGLSLKRMAVIGDSLNDRSMFEKVQYSFAMSKSPDELKLLATEIGTKTNRFRFSSLVDLITEKIIN" }, "paralog": false, "identity": 0.9908675799086758, "score": 437.0, "evalue": 3.13e-158, "description": "Internal stop codon at 314881. Nonsense mutation around 314881.", "psc": { "uniref90_id": "UniRef90_P75511", "gene": "Cof", "product": "Putative phosphatase MPN_264", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P47371", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75511", "EC:3.1.3.-", "UniRef:UniRef50_P47371", "COG:COG0561", "COG:HR", "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P47371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47371" ], "product": "Putative phosphatase MG125" } }, "id": "GIHJHMOALN_595", "locus": "GCA009939745_00560" }, { "type": "cds", "sequence": "contig_1", "start": 315332, "stop": 316369, "strand": "-", "frame": 3, "gene": "trpS", "product": "tryptophan--tRNA ligase", "db_xrefs": [ "COG:COG0180", "COG:J", "EC:6.1.1.2", "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436", "SO:0001217", "UniParc:UPI0001DD650A", "UniRef:UniRef100_A0AB38W7A3", "UniRef:UniRef50_P75510", "UniRef:UniRef90_P75510" ], "nt": "ATGAAACGCGCTCTTACCGGGATTCAAGCATCGGGCAAACAGCATTTAGGCAACTATTTGGGGGTAATGCAGTCGTTAATTGAACTCCAAGAGCAATGCCAACTGTTTGTATTTGTCGCTGATTTACACTCAATTACCGTTGATTTTCAACCCCAAGCACTGAAACAAAATAACTTTGATTTGGTACGTACTTTATTAGCGGTTGGTCTAGATCCACAAAAAGCTTGTCTCTTTTTACAAAGCGATCTTTTGGAGCACAGTATGATGGGTTATTTAATGATGGTGCAAAGCAATCTCGGTGAACTGCAACGGATGACGCAGTTCAAGGCCAAAAAAGCGGAACAAACCCGTAACCCAAACGGCACCTTAAACATTCCCACCGGACTGTTAACTTATCCAGCCCTAATGGCGGGAGATATCTTGCTGTACCAACCTGACATTGTGCCCGTTGGTAATGACCAAAAACAACACCTAGAGCTTACTCGTGATTTAGCGCAGCGAATTCAAAAGAAGTTTAAGCTCAAGCTGCGTCTACCCCAGTTTGTCCAAAACAAAGATACCAACCGTATTATGGACTTATTTGACCCAACCAAAAAGATGAGTAAGTCCTCAAAGAACCAAAACGGAGTAATTTACTTGGATGACCCAAAAGAAGTAGTGGTTAAAAAAATTCGTCAAGCTACCACTGACAGCTTTAACAAAATCCGTTTTGCTTCCAAAACGCAGCCAGGGGTGACCAATATGCTCACAATTCTCAAGGCACTTTTAAAAGAGCCTGTCAATCAATCCTTAACCAATCAGTTAGGGAATGATCTAGAAGCTTACTTTAGTACTAAATCTTATTTAGATCTCAAAAACGCACTTACAGAAGCAACCGTTAATCTGCTAGTTAACATTCAGAGAAAACGGGAACAGATTAGTCGTGAACAGGTCTTTAACTGCCTCCAGGCGGGTAAAAATCAAGCCCAAGCAACGGCCAGAACAACACTAGCACTTTTCTATGATGGCTTTGGACTAGGAAGCCAAAACATCAAGTAA", "aa": "MKRALTGIQASGKQHLGNYLGVMQSLIELQEQCQLFVFVADLHSITVDFQPQALKQNNFDLVRTLLAVGLDPQKACLFLQSDLLEHSMMGYLMMVQSNLGELQRMTQFKAKKAEQTRNPNGTLNIPTGLLTYPALMAGDILLYQPDIVPVGNDQKQHLELTRDLAQRIQKKFKLKLRLPQFVQNKDTNRIMDLFDPTKKMSKSSKNQNGVIYLDDPKEVVVKKIRQATTDSFNKIRFASKTQPGVTNMLTILKALLKEPVNQSLTNQLGNDLEAYFSTKSYLDLKNALTEATVNLLVNIQRKREQISREQVFNCLQAGKNQAQATARTTLALFYDGFGLGSQNIK", "aa_hexdigest": "56a41ca3b949572a4723ff93bd46b54e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD650A", "uniref100_id": "UniRef100_A0AB38W7A3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD650A", "UniRef:UniRef100_A0AB38W7A3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7A3", "uniref90_id": "UniRef90_P75510" }, "psc": { "uniref90_id": "UniRef90_P75510", "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_P75510", "cog_id": "COG0180", "cog_category": "J", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_P75510", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75510" ], "product": "Tryptophan--tRNA ligase" }, "genes": [ "trpS" ], "id": "GIHJHMOALN_596", "locus": "GCA009939745_00561" }, { "type": "cds", "sequence": "contig_1", "start": 316429, "stop": 316866, "strand": "+", "frame": 1, "gene": "arsC", "product": "putative MG127-like protein", "db_xrefs": [ "COG:COG1393", "COG:P", "GO:0016491", "KEGG:K16509", "RefSeq:WP_010874623.1", "SO:0001217", "UniParc:UPI0000139303", "UniRef:UniRef100_P75509", "UniRef:UniRef50_P75509", "UniRef:UniRef90_P75509" ], "nt": "ATGCTTAAGAAAAAAGTTAATAATGATGCTGGTAAGACGTTTATCTTAATTTCATCATCTTGCTCGTCTTGCCAAAAGGCAATCGAATACTTTAAGGAAAATAATTTGAGTTATACGATCGAAAACTTTTACAAAAAACCGATAAGTGACAAGCGCTTTCGTGACATTTTAAGTTTGAGTGAAGATGGCACCGAAAGTCTGTTTTCCAAGCGTGCTGACCAAATTCGTAGTAACACTAATCAATCAGTGGAAGATTTGACCATTCCTGAATTGATTAAGTTAATTCGCGAACGTCCTTCAATTTTGCGTCGACCAATTATTATCCAGTACAACTCTTCGGGGATTCCCAAGCGGATGCGCATTGGTTATAACGCCGCTGAAATTAAGGTATTTGAACGCAGCTTAATGGAACCTAAAGTGCGTACAGTAAAGAAGTAA", "aa": "MLKKKVNNDAGKTFILISSSCSSCQKAIEYFKENNLSYTIENFYKKPISDKRFRDILSLSEDGTESLFSKRADQIRSNTNQSVEDLTIPELIKLIRERPSILRRPIIIQYNSSGIPKRMRIGYNAAEIKVFERSLMEPKVRTVKK", "aa_hexdigest": "f928a8bde45f22565b6347623ee5e9b0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139303", "ncbi_nrp_id": "WP_010874623.1", "uniref100_id": "UniRef100_P75509", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139303", "RefSeq:WP_010874623.1", "UniRef:UniRef100_P75509" ] }, "ips": { "uniref100_id": "UniRef100_P75509", "uniref90_id": "UniRef90_P75509" }, "psc": { "uniref90_id": "UniRef90_P75509", "gene": "ArsC", "product": "Uncharacterized protein MG127 homolog", "uniref50_id": "UniRef50_P75509", "cog_id": "COG1393", "cog_category": "P", "kegg_orthology_id": "K16509", "go_ids": [ "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75509", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75509" ], "product": "Uncharacterized protein MG127 homolog" }, "genes": [ "arsC" ], "id": "GIHJHMOALN_597", "locus": "GCA009939745_00562" }, { "type": "cds", "sequence": "contig_1", "start": 316866, "stop": 317645, "strand": "+", "frame": 3, "gene": "nadK", "product": "NAD(+) kinase", "db_xrefs": [ "COG:COG0061", "COG:H", "EC:2.7.1.23", "GO:0003951", "GO:0005524", "GO:0005737", "GO:0006741", "GO:0019674", "GO:0046872", "GO:0051287", "KEGG:K00858", "RefSeq:WP_010874624.1", "SO:0001217", "UniParc:UPI00001320B9", "UniRef:UniRef100_P75508", "UniRef:UniRef50_P75508", "UniRef:UniRef90_P75508" ], "nt": "ATGAAGTACAAGATTTTTGCATCAACAACACCACAAACCGAGCCAGTTTTGCAAAAGTTAAAGCAGGTGTTGAAAGGTTGTGAGGCAGTTGAAAAGGGCTTTGATTATCTCTTTGTATTAGGTGGTGATGGTTTCTTTGTTTCTACTGTGGCCAACTATAACTGCCACAATTGTAGGGTAGTAGGGATTAACACTGGTCATCTAGGTTTTTACACATCGTTTAATGAAAAGGATTTGGATGATAATTTCCTCCAAAAGCTCCAGCAATGTCACTTTCAACGGATTAGTTTGTTGGAAGTTAGTGTCAATGGCCAGCAGCACTTGGTTTTGAATGAACTGGCAGTTTACACCAACACAGCTTACCCAATTAACATCTTTATTGATGGTGAAGCGTGGGAGTTTTACCGCGGTTCAGGTTTATTAATCGGTCCGCGTACTGGTTCGACTGCACTGGCTAAATCTGCTAAAGGGGCGGTGATTTTCCCTGGCATTGATGTGCTGCAAATTATTGAAATGAATCCACTTTTGCACCCCAATCAAGTTACTATCCAATCACCAATTATTTTGCCCAAAGAAACGCAAGTGGAATTTGTGGTCAAAAAAGCCTTTAACCCACAACAGTTTCCCACCTTTTACTGTGACGGGAGAAAGTTGGAATTGCCAAACGCTGACACAACTTTAGCGCTTAAACTAGTGCAATCCACACCGATGTTTAACATTTCACTCAAAACACAGGACTTTATCAATAAACTTAAGTCCACTTTTATTAAACAAAGCTAA", "aa": "MKYKIFASTTPQTEPVLQKLKQVLKGCEAVEKGFDYLFVLGGDGFFVSTVANYNCHNCRVVGINTGHLGFYTSFNEKDLDDNFLQKLQQCHFQRISLLEVSVNGQQHLVLNELAVYTNTAYPINIFIDGEAWEFYRGSGLLIGPRTGSTALAKSAKGAVIFPGIDVLQIIEMNPLLHPNQVTIQSPIILPKETQVEFVVKKAFNPQQFPTFYCDGRKLELPNADTTLALKLVQSTPMFNISLKTQDFINKLKSTFIKQS", "aa_hexdigest": "2a76f8b4329ae23c917b427157968987", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001320B9", "ncbi_nrp_id": "WP_010874624.1", "uniref100_id": "UniRef100_P75508", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001320B9", "RefSeq:WP_010874624.1", "UniRef:UniRef100_P75508" ] }, "ips": { "uniref100_id": "UniRef100_P75508", "uniref90_id": "UniRef90_P75508" }, "psc": { "uniref90_id": "UniRef90_P75508", "gene": "nadK", "product": "NAD(+) kinase", "ec_ids": [ "2.7.1.23" ], "uniref50_id": "UniRef50_P75508", "cog_id": "COG0061", "cog_category": "H", "kegg_orthology_id": "K00858", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0051287", "GO:0003951", "GO:0019674", "GO:0006741" ] }, "pscc": { "uniref50_id": "UniRef50_P75508", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75508" ], "product": "NAD kinase" }, "genes": [ "nadK" ], "id": "GIHJHMOALN_598", "locus": "GCA009939745_00563" }, { "type": "cds", "sequence": "contig_1", "start": 317672, "stop": 317998, "strand": "+", "frame": 2, "gene": "ptsG2", "product": "Putative phosphotransferase enzyme IIB component MPN_268", "db_xrefs": [ "COG:COG1264", "COG:G", "EC:2.7.1.-", "GO:0008982", "GO:0009401", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75507", "UniRef:UniRef90_P75507" ], "nt": "GTGTTGAGCTTTGGCTTACTTTACCTTTATTTGGTAAAACGTGCTAAGAGGGCCGCGCTACAATTAAACAACAAACTAGTCGAATCACACACAATTCCTTTTGCTGTACGTGACTTTATTGCCGCTTGCGGGGGTAGAACTAACTTTGTTAGTTTACGCACCTCACCCACCCAATTAATTGTTAGTTTTGCCAAGCCAGAGTTAGTTCAAATTGCCGCTTTGCAAAAGCTTGGAATTAAAGGGATTAATAAGAGTCAGAACCAGTACCGCTTTGTATTGGGCAATTTTGTCAATCAATTAAAACAGCAAATTGAAAATGAGCGCTAA", "aa": "MLSFGLLYLYLVKRAKRAALQLNNKLVESHTIPFAVRDFIAACGGRTNFVSLRTSPTQLIVSFAKPELVQIAALQKLGIKGINKSQNQYRFVLGNFVNQLKQQIENER", "aa_hexdigest": "13d04c6ff2e45ac7835b0b3ce2bab351", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75507", "query_cov": 0.9907407407407407, "subject_cov": 0.9145299145299145, "identity": 1.0, "score": 204.0, "evalue": 1.39e-66, "valid": true, "gene": "PtsG2", "product": "Putative phosphotransferase enzyme IIB component MPN_268", "ec_ids": [ "2.7.1.-" ], "uniref50_id": "UniRef50_P75507", "cog_id": "COG1264", "cog_category": "G", "go_ids": [ "GO:0016020", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75507", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75507" ], "product": "Putative phosphotransferase enzyme IIB component MPN_268" }, "genes": [ "ptsG2" ], "id": "GIHJHMOALN_599", "locus": "GCA009939745_00564" }, { "type": "cds", "sequence": "contig_1", "start": 317988, "stop": 319301, "strand": "+", "frame": 3, "gene": "rny", "product": "Ribonuclease Y", "db_xrefs": [ "COG:COG1418", "COG:JR", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0005886", "GO:0006402", "SO:0001217", "UniRef:UniRef50_P47376", "UniRef:UniRef90_P75506" ], "nt": "ATGAGCGCTAAGTTAACGTTAGAAAGCATTGCAAAAACATTTGCCGAAACATCGATCTTTGCGATCTTGTTTTTAATTATTGTCATCCTCAATTTAGGTTCACTTGTTTTTTTAGCGTATCAGTACCGTGTTTACAAAAAGAAGCAAAGAGCAAACCTCACCAAGCAGACTTATAACAATGACTATGGCCAAATTGTTAATTTAAAACAGCAAAACGCTGCCAAGATAAAGGAGCTTGCTAACTTAAAAGATCAGTTAAGTGAATTAGGCCAAAAATTTAACACCACACTGAGTGAAATCATTAACAAACCACTTGTCAATGTGATTGATGAATACTTGGATGAACAGTTTAAACAAGCTGCTAATTTTCGTGAAGCAGAACTTAATGCCGTTTTAGATAGTAATGACCAGAAAACAGTGTTCCATAAGCGTTTGTTCAATAAATTTCACTTTGGTGTCGACAAGCTAGCTAATATTAATGTCAAAAATCCGCTCAATTTATGCTGGGTTGACAGCGCTAGTTTTACCGTGATTGAAAGTGATTTCCGCAAACTTAATGGTGTTGGCGGGATTAATAAAAAGTTGTTAATTGAAAAGTTAAGGATTGAAGATATTATCTTTACCAATATCGATAAGAAGTACTATGAGGTACAGATACTATCTGACTCGCCAGTAAAGGTACAAAAAACAGTGTTAACTATTCGCAACATCTTGATTAATGATTACGTGGACAATGAAAAAATTGAGTCATATGCTAGGGAAGCTAACGGTTACTTCAATGACCACTGTAAACTGATTGGTAAGCAGGTGTTAGAACGACTCAATATCTTTGAAATTAGCCCTAAACTACACAAGTTTTTTGGTTTATTAGCGTTTCGTTATTCTTTCGGTCAAAACGTTTTATCACACTGTCTGGAAACTGGTTTTCTCACCGCTTACCTAGCGCTTTTAGTTAACTTTAAACCTGATGTTGCATTAAAGTGCGGTTTGTATCATGATATTGGTAAAGCGGATGATGAAAACGGGAAGAAGAACCACACGGTTACAGGTGCCAAGATCGGTGATGAATTTTACTTTGAAAACGATGTCAAATACACAATTGCCAACCACCATAATAAAAATGTCGATAACGTATACTGTCGTTTAACTCAAATTGGCGATAAGCTTTCAGCAGGGCGTTTGGGTGCGCGCTCTGACAGTTCGGTTTTATTTAACCAACTCAAACAAGAATTAAAGCAAATTGTGGAAGAAACACTAGCACAATTTAAAACAACCATCCTTTTAGGACAAAGTGGACGCCGATTAGTAATTTGA", "aa": "MSAKLTLESIAKTFAETSIFAILFLIIVILNLGSLVFLAYQYRVYKKKQRANLTKQTYNNDYGQIVNLKQQNAAKIKELANLKDQLSELGQKFNTTLSEIINKPLVNVIDEYLDEQFKQAANFREAELNAVLDSNDQKTVFHKRLFNKFHFGVDKLANINVKNPLNLCWVDSASFTVIESDFRKLNGVGGINKKLLIEKLRIEDIIFTNIDKKYYEVQILSDSPVKVQKTVLTIRNILINDYVDNEKIESYAREANGYFNDHCKLIGKQVLERLNIFEISPKLHKFFGLLAFRYSFGQNVLSHCLETGFLTAYLALLVNFKPDVALKCGLYHDIGKADDENGKKNHTVTGAKIGDEFYFENDVKYTIANHHNKNVDNVYCRLTQIGDKLSAGRLGARSDSSVLFNQLKQELKQIVEETLAQFKTTILLGQSGRRLVI", "aa_hexdigest": "5e960d97b12ececc64f2e46c2c8d9e84", "start_type": "ATG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_P75506", "query_cov": 1.0091533180778032, "subject_cov": 0.8873239436619719, "identity": 0.973, "score": 823.0, "evalue": 9.65e-300, "valid": true, "gene": "rny", "product": "Ribonuclease Y", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47376", "cog_id": "COG1418", "cog_category": "JR", "go_ids": [ "GO:0005886", "GO:0003723", "GO:0004521", "GO:0006402" ] }, "pscc": { "uniref50_id": "UniRef50_P47376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47376" ], "product": "Ribonuclease Y" }, "genes": [ "rny" ], "id": "GIHJHMOALN_600", "locus": "GCA009939745_00565" }, { "type": "cds", "sequence": "contig_1", "start": 319302, "stop": 319469, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAACCAACCAGCACAATAACATTATTGACAACCAACAGTTAACTGATTTGGCTACTACCATTAAAAGCAAAATCGTGCAAAACAACATTACTAATCGCTTTCCAATTAAGGTAGTATTACGCTATAACTTCGAACACAGCTTTGATACAAAGGACAAGAACTAG", "aa": "METNQHNNIIDNQQLTDLATTIKSKIVQNNITNRFPIKVVLRYNFEHSFDTKDKN", "aa_hexdigest": "a8d39b1aa755cf66f792235a554d7531", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6504.217700000001, "isoelectric_point": 8.140245628356933 }, "id": "GIHJHMOALN_601", "locus": "GCA009939745_00566" }, { "type": "cds", "sequence": "contig_1", "start": 319472, "stop": 319759, "strand": "+", "frame": 2, "gene": null, "product": "putative MG131-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874627.1", "SO:0001217", "UniParc:UPI000013931E", "UniRef:UniRef100_Q9EXD1", "UniRef:UniRef50_Q9EXD1", "UniRef:UniRef90_Q9EXD1" ], "nt": "ATGCAATACACCGTTTTAATTCCACTGTTTATTTTTATAGGAGCCATGGTGTTGTTTGGTTTTAGCTTTCAGAAAAAGCAACCACAACGCCGCATCGTTCAAATTCTGTTCTTAGCTTACTGTGTGGATTTTTTAGCACTAATTATTGCAGTGATGATGCTCACATTCTTAAGTTATGATGACTTAATGCTGGGCGTTTTAATTCCAGTATTAATCCTTTCAATCATTATGTTCTTTGTGATGATTATTGCCCACTATCCACTAATGAAACGCTTGTTTGGACATTAG", "aa": "MQYTVLIPLFIFIGAMVLFGFSFQKKQPQRRIVQILFLAYCVDFLALIIAVMMLTFLSYDDLMLGVLIPVLILSIIMFFVMIIAHYPLMKRLFGH", "aa_hexdigest": "c68edcf8234a323dcf7d6b19cf7a548a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013931E", "ncbi_nrp_id": "WP_010874627.1", "uniref100_id": "UniRef100_Q9EXD1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013931E", "RefSeq:WP_010874627.1", "UniRef:UniRef100_Q9EXD1" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD1", "uniref90_id": "UniRef90_Q9EXD1" }, "psc": { "uniref90_id": "UniRef90_Q9EXD1", "product": "Uncharacterized protein MG131 homolog", "uniref50_id": "UniRef50_Q9EXD1", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD1" ], "product": "Uncharacterized protein MG131 homolog" }, "genes": [], "id": "GIHJHMOALN_602", "locus": "GCA009939745_00567" }, { "type": "cds", "sequence": "contig_1", "start": 320005, "stop": 320199, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTTTTGCCTTCTTTAGTTTTAATTGCTTTAGACAAATCAATGTCTTTACCAGTGGTTTCTTTAATAAGATTTCTAAGATCAGCTTGGATACCAGTGCAAGCACTTAACAATGTTCCACATAATAAAAATAGAGAACTAAAAGAAAACCTTGACAAAAACTTCTTTTTACGCATTAACAGTAAAAAATAA", "aa": "MFFLPSLVLIALDKSMSLPVVSLIRFLRSAWIPVQALNNVPHNKNRELKENLDKNFFLRINSKK", "aa_hexdigest": "fb79d38aa40892fca75e530f2cfb7137", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7465.829599999998, "isoelectric_point": 10.620408821105958 }, "id": "GIHJHMOALN_603", "locus": "GCA009939745_00568" }, { "type": "cds", "sequence": "contig_1", "start": 320356, "stop": 320445, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGGTTGATCATTTTCAAATGACATACCAAAGTTTTAAGGATTTGTTAGTTGAGGCTAGGCTGAGCTATACCTTTAATTGA", "aa": "MERKVDHFQMTYQSFKDLLVEARLSYTFN", "aa_hexdigest": "e34c401a42d6c8923a0331cea5fd4f57", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3598.0693, "isoelectric_point": 6.518261528015137 }, "id": "GIHJHMOALN_604", "locus": "GCA009939745_00569" }, { "type": "cds", "sequence": "contig_1", "start": 320465, "stop": 320617, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTCACTGCTAAGAGAGGTGACAAGCATTATTTTTATCTTTTCCTTAAAATTAAATCTGATCCAAAAAAACAATTTTCAGCAAAAAAATTTCTTACTGAAGGAGAAGCGTTTACTGATCAAGAAGGAAAACAAACTACAAGAAATTTAG", "aa": "MISLLREVTSIIFIFSLKLNLIQKNNFQQKNFLLKEKRLLIKKENKLQEI", "aa_hexdigest": "fff8c858299082528508dd4a4a4b764c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6060.288099999998, "isoelectric_point": 10.214515876770019 }, "id": "GIHJHMOALN_605", "locus": "GCA009939745_00570" }, { "type": "cds", "sequence": "contig_1", "start": 321793, "stop": 322044, "strand": "-", "frame": 1, "gene": null, "product": "Restriction-modification enzyme subunit S1B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W825", "UniRef:UniRef90_A0AB38W825" ], "nt": "TTGCAAACATCTAAGTTTGTTAATTATGCTTGCCCTATTCCTAAATTAGGAATAAGTAAATTAGCAGAAATTGAACTTACTTTCCCACCTCTAGAAATTCAAGAAAAGATTGCCGATATCCTCTTTGCTTTTGAAAAAAATGTAACGATCTTACCGAAAGTATTCCCAAAGAAATTGAAATGCGTAAAAAGCAATTGGATTATTACCAAAACTATTTATTCGATTGAGTTCAAAAACAAAAAGAAGGCTTAA", "aa": "MQTSKFVNYACPIPKLGISKLAEIELTFPPLEIQEKIADILFAFEKNVTILPKVFPKKLKCVKSNWIITKTIYSIEFKNKKKA", "aa_hexdigest": "6b9dc874c7682bf42e295363c3f2e7a2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 321793, "stop": 322236, "observations": { "mutation": [ 322176 ], "stop": [ 322176 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W825", "query_cov": 1.0, "subject_cov": 0.564625850340136, "identity": 0.988, "score": 166.0, "evalue": 2.25e-51, "gene_start": 1, "gene_end": 83, "reference_start": 65, "reference_end": 147, "reference_sequence": "MNDGEVGKIKDCDRDGEYVTWTTGGSYAGAVFYRKGQFNARQDCGVLKVKNEEINPKFLAYALRLQTSKFVNYACPIPKLGISKLAEIELTFPPLEIQEKIADILFAFEKNVTILPKVFPKKLKCVKSNWIITKTIYSIEFKNKKKA" }, "paralog": false, "identity": 0.9863945578231292, "score": 295.0, "evalue": 1.39e-104, "description": "Internal stop codon at 322176. Nonsense mutation around 322176.", "psc": { "uniref90_id": "UniRef90_A0AB38W825", "product": "Restriction-modification enzyme subunit S1B", "uniref50_id": "UniRef50_A0AB38W825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W825", "UniRef:UniRef50_A0AB38W825" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W825" ], "product": "Restriction-modification enzyme subunit S1B" } }, "id": "GIHJHMOALN_606", "locus": "GCA009939745_00571" }, { "type": "cds", "sequence": "contig_1", "start": 322480, "stop": 322683, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_272", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_Q9EXD2", "UniRef:UniRef90_Q9EXD2" ], "nt": "ATGAACCGTCCTACTCCCAATTTTGAAGCAATCGATAAGAAGATTAGTGCCTTTGTCACTAACCACGATAACTTATTGGATAAGTTACTCAAACAACAAACCGAATTGTTAACTAGTGAAATTACGACTAATTTTGAGGTAACCCAACAAATCCAGGAAGAAGTAGCCAAAAAGACGAAGCAACACTCTAAAAACTACAAGTGA", "aa": "MNRPTPNFEAIDKKISAFVTNHDNLLDKLLKQQTELLTSEITTNFEVTQQIQEEVAKKTKQHSKNYK", "aa_hexdigest": "669edc04f61a0ea699ba98706a6e0463", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 322480, "stop": 322761, "observations": { "mutation": [ 322681 ], "stop": [ 322681 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9EXD2", "query_cov": 1.0, "subject_cov": 0.7204301075268817, "identity": 1.0, "score": 128.0, "evalue": 1.89e-37, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MNRPTPNFEAIDKKISAFVTNHDNLLDKLLKQQTELLTSEITTNFEVTQQIQEEVAKKTKQHSKNYKWLVTVVLANGVVSLFLLGGLIYLFSK" }, "paralog": false, "identity": 0.978494623655914, "score": 177.0, "evalue": 3.76e-59, "description": "Internal stop codon at 322681. Nonsense mutation around 322681.", "psc": { "uniref90_id": "UniRef90_Q9EXD2", "product": "Uncharacterized protein MPN_272", "uniref50_id": "UniRef50_Q9EXD2", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9EXD2", "UniRef:UniRef50_Q9EXD2", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD2" ], "product": "Uncharacterized protein MPN_272" } }, "id": "GIHJHMOALN_607", "locus": "GCA009939745_00572" }, { "type": "cds", "sequence": "contig_1", "start": 322738, "stop": 323172, "strand": "-", "frame": 1, "gene": "hinT", "product": "putative HIT-like protein", "db_xrefs": [ "COG:COG0537", "COG:FR", "GO:0003824", "GO:0009117", "RefSeq:WP_014325433.1", "SO:0001217", "UniParc:UPI0001DD650D", "UniRef:UniRef100_A0AAX0SPP5", "UniRef:UniRef50_P47378", "UniRef:UniRef90_P75504" ], "nt": "ATGGTGCAAAAACAAAGTATGGCAAACAATAACTGTATCTTCTGTGGAATTGTAGAGGGGAATGTGAAATCCTTTAAGGTTGGTGAAAACGAACACGCTGTTGCCTTTTTAGATGCCTTTCCAGTTGCTGATGGTCATACGCTTGTAATTCCCAAAAAACACGCCGTTAACTATTCCTCGACTGATGATGAGAGTTTGAAGGCGGTTAGTTTACTCGCCAAAGAAATGGCTTTAAAGCTACAACAACGGCTCCAACCAGCTGGATTAAACTATGTTGTCAATGAAGGGGCTAAGGCAGGGCAGGAAGTATTTCACTACCACATGCATGTAGTACCAAAATACGAAACTGGTTTAGGCTTTTGTTACAACGTTAAAAAGACGAACAACCGCTCAATTGAAGCTAATTGGGAACTACTTACTAAAGAGGTAGATTAA", "aa": "MVQKQSMANNNCIFCGIVEGNVKSFKVGENEHAVAFLDAFPVADGHTLVIPKKHAVNYSSTDDESLKAVSLLAKEMALKLQQRLQPAGLNYVVNEGAKAGQEVFHYHMHVVPKYETGLGFCYNVKKTNNRSIEANWELLTKEVD", "aa_hexdigest": "4b99a9b8fd1a3154440d371ca496d6d7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD650D", "ncbi_nrp_id": "WP_014325433.1", "uniref100_id": "UniRef100_A0AAX0SPP5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD650D", "RefSeq:WP_014325433.1", "UniRef:UniRef100_A0AAX0SPP5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SPP5", "uniref90_id": "UniRef90_P75504" }, "psc": { "uniref90_id": "UniRef90_P75504", "gene": "HinT", "product": "Uncharacterized 16.1 kDa HIT-like protein", "uniref50_id": "UniRef50_P47378", "cog_id": "COG0537", "cog_category": "FR", "go_ids": [ "GO:0003824", "GO:0009117" ] }, "pscc": { "uniref50_id": "UniRef50_P47378", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47378" ], "product": "Uncharacterized HIT-like protein MG132" }, "genes": [ "hinT" ], "id": "GIHJHMOALN_608", "locus": "GCA009939745_00573" }, { "type": "cds", "sequence": "contig_1", "start": 323240, "stop": 323419, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCGAGCCGCTACATGAATTGGGTAAGTTTTTACTCAAGGCAGGGGTGTACCCAAGCCTTTATGCCTTTTATTTACAAACAGTACCTTTTCTAAAGATATGGGACAACGGGCATGATTCGTACTCGGTCTATGGTTTCTTCTCCCAAACCAAATATAACAGCTATGTTTGGTTTTGA", "aa": "MREPLHELGKFLLKAGVYPSLYAFYLQTVPFLKIWDNGHDSYSVYGFFSQTKYNSYVWF", "aa_hexdigest": "6600a0c7effaa00232f4fb117449c3e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7063.991900000001, "isoelectric_point": 8.003379249572756 }, "id": "GIHJHMOALN_609", "locus": "GCA009939745_00574" }, { "type": "cds", "sequence": "contig_1", "start": 323504, "stop": 323941, "strand": "-", "frame": 3, "gene": null, "product": "putative MG133-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47379", "UniRef:UniRef90_P75503" ], "nt": "ATGAAAAAAACAATAGGACTCGCTTACCGTTTTTTCTATCTTAACAATAATTGCGACTTTTATTTACTTTTTCTCGCGCCGTTTTCCCTGTTTAACCTTGGGGTAATGGTAGCTAGTGCTGTTATCAGTGTGGTTTATAACAACCAACCACAATTAATCTGGTTTACTAACTTTGATACCTTTACCTACCAGAGCAACACAATTGCCGCTGTTTGTGTCTTAATGTACCTCTGTAAACGACGCTGTAAGCTGTTTGACAATAATGCGTTGTTTTTAAGTGCTGCAGGTTACTTGGTCTTTACCGTCATCTTTTTTAACCTGTATGTACTGTCACGTGTTACTGGATTTGTTAATGTAGAAGAACACGTTAAGGGTTGGTTTTCGACTATTACGAGTGAAATGCCATACAGTTTTAGTGGTAATCCTTTAACTGATTGA", "aa": "MKKTIGLAYRFFYLNNNCDFYLLFLAPFSLFNLGVMVASAVISVVYNNQPQLIWFTNFDTFTYQSNTIAAVCVLMYLCKRRCKLFDNNALFLSAAGYLVFTVIFFNLYVLSRVTGFVNVEEHVKGWFSTITSEMPYSFSGNPLTD", "aa_hexdigest": "169616fd36fea1a5825ef43ee09f50b5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 323201, "stop": 323941, "observations": { "mutation": [ 323242, 323443, 323506 ], "stop": [ 323242, 323443, 323506 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75503", "query_cov": 1.0, "subject_cov": 0.5451127819548872, "identity": 1.0, "score": 293.0, "evalue": 5.94e-99, "gene_start": 1, "gene_end": 145, "reference_start": 1, "reference_end": 145, "reference_sequence": "MKKTIGLAYRFFYLNNNCDFYLLFLAPFSLFNLGVMVASAVISVVYNNQPQLIWFTNFDTFTYQSNTIAAVCVLMYLCKRRCKLFDNNALFLSAAGYLVFTVIFFNLYVLSRVTGFVNVEEHVKGWFSTITSEMPYSFSGNPLTDWISFAQLFLHVIYPASFFGFIWIFFKTYKMREPLHELGKFLLKAGVYPSLYAFYLQTVPFLKIWDNGHDSYSVYGFFSQTKYNSYVWFWSIPIFASMFLILWGLFVINNRYYGAKTKYGKQ" }, "paralog": false, "identity": 0.9878048780487805, "score": 497.0, "evalue": 1.79e-181, "description": "Internal stop codon at 323242, 323443, 323506. Nonsense mutation around 323242, 323443, 323506.", "psc": { "uniref90_id": "UniRef90_P75503", "product": "Uncharacterized protein MG133 homolog", "uniref50_id": "UniRef50_P47379", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75503", "UniRef:UniRef50_P47379", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47379", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47379" ], "product": "Uncharacterized protein MG133" } }, "id": "GIHJHMOALN_610", "locus": "GCA009939745_00575" }, { "type": "cds", "sequence": "contig_1", "start": 323963, "stop": 324265, "strand": "+", "frame": 2, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "KEGG:K09747", "RefSeq:WP_010874632.1", "SO:0001217", "UniParc:UPI000013932F", "UniRef:UniRef100_P75502", "UniRef:UniRef50_P47380", "UniRef:UniRef90_P75502" ], "nt": "ATGAGTTTTAAAAAGATTACCGAAATGATGCGCCAAGCTGAACGACAAAGCAAGCAAAAAGCGCTTGATTTCGAGCAAAAATTGTTTGAGTACAGCTATAAGAATGCTGCCATCAAGATTATCATTTTTGGTAATCTCACCATTAAGTCTATCACAATCGATCCCGCATTAATTGACCCAGAAGACAAGGTCACTTTAGAGGAGATGATTACAGAAGCTGTCAATGAGGCTGTGGGTGATGTTAAGGCCAAGTACGACCAGTTAATGGAAGAGGCTATGCCACAGATGCCTGGGCTTTTTTAA", "aa": "MSFKKITEMMRQAERQSKQKALDFEQKLFEYSYKNAAIKIIIFGNLTIKSITIDPALIDPEDKVTLEEMITEAVNEAVGDVKAKYDQLMEEAMPQMPGLF", "aa_hexdigest": "85a8ee1bfe713a32c945844c5f7bc40e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013932F", "ncbi_nrp_id": "WP_010874632.1", "uniref100_id": "UniRef100_P75502", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013932F", "RefSeq:WP_010874632.1", "UniRef:UniRef100_P75502" ] }, "ips": { "uniref100_id": "UniRef100_P75502", "uniref90_id": "UniRef90_P75502" }, "psc": { "uniref90_id": "UniRef90_P75502", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_P47380", "cog_id": "COG0718", "cog_category": "K", "kegg_orthology_id": "K09747", "go_ids": [ "GO:0003677" ] }, "pscc": { "uniref50_id": "UniRef50_P47380", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47380" ], "product": "Uncharacterized protein MG134" }, "genes": [ "ybaB" ], "id": "GIHJHMOALN_611", "locus": "GCA009939745_00576" }, { "type": "cds", "sequence": "contig_1", "start": 324237, "stop": 324422, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCCTCAAGCTAGATCCAAAAAATGCTGATTTAATCTTTGGAGTTAGCTTTGGGTTGAGCTATTTCGCCATTGTATTGTTGACAGTTCTCGTCAACATTCCAACCTTTATACAGCTCACTAAAGATAAACAAATGGTTAAGCTAGCACTGCAGCTTAAAAAAGCCCAGGCATCTGTGGCATAG", "aa": "MFLKLDPKNADLIFGVSFGLSYFAIVLLTVLVNIPTFIQLTKDKQMVKLALQLKKAQASVA", "aa_hexdigest": "70b638538c507ceb45fa1c599637e4d5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6754.133800000002, "isoelectric_point": 9.778516197204588 }, "id": "GIHJHMOALN_612", "locus": "GCA009939745_00577" }, { "type": "cds", "sequence": "contig_1", "start": 324450, "stop": 324548, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGCGCTAGCATATCTCAGTTATGTGGCCTTGATGGTTAACATCTTTAAGGGACAAATTACCGGTAATACTACCGGCATTTTGGCTGAAGATTGA", "aa": "MFALAYLSYVALMVNIFKGQITGNTTGILAED", "aa_hexdigest": "8ae28675fb334f1ce0469fc7933e585d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3466.0291000000007, "isoelectric_point": 4.667413520812987 }, "id": "GIHJHMOALN_613", "locus": "GCA009939745_00578" }, { "type": "cds", "sequence": "contig_1", "start": 324588, "stop": 325094, "strand": "-", "frame": 2, "gene": null, "product": "putative MG135-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47381", "UniRef:UniRef90_P75501" ], "nt": "ATGCAGTCGCTTAACTATCTTGTAGTCATCCTAACAGTAGCCGGGGTGCTAGTTATCTTGGGATTTACCCCATTAATTCGCAAATTAAAAATCCAGTTTTATTGTTTACAAGTTTTTGCAGCTATTCTCTTTCTCTATGTCTTTTTCGGGCGGCAAATTATCTATATTTTTCCCGATATTTACGGCACTGCAGCCAAGGCTAAGAATGCTGTTGCAAATGTTCCTTTAGATTCACTAAGGTTGAGCAGGATCTTTTTGTTAGATCTCTGCCCCTTCTTTGCCTTAATCGGCCCGATCTTTATCTTTTTACGCCAGAAAAAGGTGGCGGGTGTGTTGGCGATCTTTGGTTTTTACGGTGCTGCAATAACCTTATTTGGCGAGTTAATTTTTACTCCATTAAAACAAGAGGAGATTGTCAAATTTCTCTTTGTTGGTTTGGAAAACAACCAGGTTTACTTCATGATGCACTTCTTGAGTTTCTTGTTGAGTCTGGCTGTCTTTTTATGA", "aa": "MQSLNYLVVILTVAGVLVILGFTPLIRKLKIQFYCLQVFAAILFLYVFFGRQIIYIFPDIYGTAAKAKNAVANVPLDSLRLSRIFLLDLCPFFALIGPIFIFLRQKKVAGVLAIFGFYGAAITLFGELIFTPLKQEEIVKFLFVGLENNQVYFMMHFLSFLLSLAVFL", "aa_hexdigest": "1c2b5ac40e079a69c01ed1fba2dcbafb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 324285, "stop": 325094, "observations": { "mutation": [ 324452, 324590 ], "stop": [ 324452, 324590 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75501", "query_cov": 1.0, "subject_cov": 0.5894736842105263, "identity": 1.0, "score": 313.0, "evalue": 3.54e-106, "gene_start": 1, "gene_end": 168, "reference_start": 1, "reference_end": 168, "reference_sequence": "MQSLNYLVVILTVAGVLVILGFTPLIRKLKIQFYCLQVFAAILFLYVFFGRQIIYIFPDIYGTAAKAKNAVANVPLDSLRLSRIFLLDLCPFFALIGPIFIFLRQKKVAGVLAIFGFYGAAITLFGELIFTPLKQEEIVKFLFVGLENNQVYFMMHFLSFLLSLAVFLWDDGFSLISFFYIHVFALAYLSYVALMVNIFKGQITGNTTGILAEDWLSGEYKNVAVFLKLDPKNADLIFGVSFGLSYFAIVLLTVLVNIPTFIQLTKDKQMVKLALQLKKAQASVA" }, "paralog": false, "identity": 0.9925650557620818, "score": 514.0, "evalue": 1.23e-187, "description": "Internal stop codon at 324452, 324590. Nonsense mutation around 324452, 324590.", "psc": { "uniref90_id": "UniRef90_P75501", "product": "Uncharacterized protein MG135 homolog", "uniref50_id": "UniRef50_P47381", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75501", "UniRef:UniRef50_P47381", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47381", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47381" ], "product": "Uncharacterized protein MG135" } }, "id": "GIHJHMOALN_614", "locus": "GCA009939745_00579" }, { "type": "cds", "sequence": "contig_1", "start": 325139, "stop": 325564, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAGCGATCGTTTAAATGATCAAGCACAAAATCGCTTGCAAAAACTCCTAAACCTCAAACAAACTGGCAACGATCCCTACCTCATTACTAAGATAGAAAACACCCACTCAGCGGCTAGTTTTCAAAAAGCATTTGCCAACCAATCTGACGCTGAGTTAAAGCAGTACCAAGTGATCTTAACCGGTCGGATTATTGCGCTCCGGCAGACCTTTATTATTATCCAAGACTTTAGTGGGAAAATGCAGTTGTACATTAATAAAAAAACTGCCCCCGAACTGTTTGAATATTTCAATAATTACATTGATTTGGGTGATCAAATAGTAGCCACTGGCCATCCAATGATGACCAAAACAGGTGTTTTAACGCTGAACGTAGAGCGATTACAAATTGTCGCTAAATGCTTACAAACCCCACCTGAAAAATGA", "aa": "MSDRLNDQAQNRLQKLLNLKQTGNDPYLITKIENTHSAASFQKAFANQSDAELKQYQVILTGRIIALRQTFIIIQDFSGKMQLYINKKTAPELFEYFNNYIDLGDQIVATGHPMMTKTGVLTLNVERLQIVAKCLQTPPEK", "aa_hexdigest": "313ab771441699e812771a1b274ce2a8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 79, "aa_cov": 0.524822695035461, "hmm_cov": 0.9493670886075949, "evalue": 1.5331075457155018e-12, "score": 42.78839874267578, "start": 58, "stop": 131 } ], "seq_stats": { "molecular_weight": 16075.34399999999, "isoelectric_point": 9.014179039001466 }, "id": "GIHJHMOALN_615", "locus": "GCA009939745_00580" }, { "type": "cds", "sequence": "contig_1", "start": 325571, "stop": 326017, "strand": "+", "frame": 2, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6F12" ], "nt": "TTGACCGATCCAGAAGCGCGAGCACGAAAGCGTTACTTGGATTTGACCTACAACCGTGCGCAGGTCGAAGTCTTTTTGAAACGCACCCAGATTATTACAGCTATCCGTACCTTTTTAAATGAAGCGGGCTTTCTAGAAGTGGAAACACCCATTTTACAAGCCGTTTTAGGGGGTGCTAACGCCAAGCCCTTTAAAACACACTATAACGCCTTAAAGGGTGATTTTTACTTGCGGATTGCAAATGAAATAGCACTCAAAAAGCTCATTGTGGGCGGTTTGCCGAAAGTGTATGAAATGGGGCGGATGTTTCGTAACGAAGGGGTGGATACTACCCATAATCCGGAGTTTACGAGCATTGAAATGTATCAGGCTAACGCTGACTATGCGGTCATGATGGATTTAACCGAAAACCTCATTAAGTTTGTTTGCAAAACACTCAATCAGTGA", "aa": "MTDPEARARKRYLDLTYNRAQVEVFLKRTQIITAIRTFLNEAGFLEVETPILQAVLGGANAKPFKTHYNALKGDFYLRIANEIALKKLIVGGLPKVYEMGRMFRNEGVDTTHNPEFTSIEMYQANADYAVMMDLTENLIKFVCKTLNQ", "aa_hexdigest": "278eab2d7f0f5af08b4da585945887c5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000AFE6F12", "query_cov": 0.8918918918918919, "subject_cov": 0.8048780487804879, "identity": 0.598, "score": 160.0, "evalue": 9.83e-48, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI000AFE6F12" }, "pscc": { "uniref50_id": "UniRef50_UPI000AFE6F12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6F12" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "GIHJHMOALN_616", "locus": "GCA009939745_00581" }, { "type": "cds", "sequence": "contig_1", "start": 326075, "stop": 326608, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGGTAGATTTAATCTGCCAAGTTATTGGGGTTAATTTTGATGATGTTAAGGATGATCAAACAGCCATTGCGCTTGCAAAGCAGCACAAAGTAGAGTTAAAAAAGCACGAGCAGAACAAACAACACATTATTAACCGTTTCTTTGAACAGTTCTGTGAGCATACCTTAATCCAACCCACCTTTGTCACACACTATCCCAAGGCCGTTTCCCCGCTAGCTAAACAAGATCCCCACAACCCCGAGTTTACTGAGCGCTTTGAACTTTTTATTAATACAAAAGAGTTAGCGAACGCTTACAGTGAGTTGAATGATCCCCTAGAACAACGTGCGCGCTTTGAACAACAGTTGCAGGAAAAACGCATGGGTAATGATGAAGCGAGTGAATTAGATGAATCCTTTTTGGATGCTTTGAGTTTTGGTCTCCCGCCAACAGGCGGCCTTGGGATTGGGGTTGATCGGTTAGTGATGTTATTGTGCGAGTGCAGTTCCATCCGTGACGTTGTTTTCTTTCCCCAACTACGTGAATTGAAATAA", "aa": "MVDLICQVIGVNFDDVKDDQTAIALAKQHKVELKKHEQNKQHIINRFFEQFCEHTLIQPTFVTHYPKAVSPLAKQDPHNPEFTERFELFINTKELANAYSELNDPLEQRARFEQQLQEKRMGNDEASELDESFLDALSFGLPPTGGLGIGVDRLVMLLCECSSIRDVVFFPQLRELK", "aa_hexdigest": "42229ba3e2e73c02465eca748c8a0689", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031D0E1C7", "query_cov": 0.9830508474576272, "subject_cov": 0.8093023255813954, "identity": 0.557, "score": 189.0, "evalue": 8.52e-58, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A349PV97", "kegg_orthology_id": "K04567" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 127, "aa_cov": 0.6892655367231638, "hmm_cov": 0.968503937007874, "evalue": 1.2216583852536869e-37, "score": 125.05966186523438, "start": 52, "stop": 173 } ], "seq_stats": { "molecular_weight": 20373.939299999987, "isoelectric_point": 5.039197349548339 }, "id": "GIHJHMOALN_617", "locus": "GCA009939745_00582" }, { "type": "cds", "sequence": "contig_1", "start": 326736, "stop": 327413, "strand": "+", "frame": 3, "gene": "glf", "product": "UDP-galactopyranose mutase", "db_xrefs": [ "COG:COG0562", "COG:M", "EC:5.4.99.9", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660", "KEGG:K01854", "SO:0001217", "UniRef:UniRef50_P75499", "UniRef:UniRef90_P75499" ], "nt": "TTGGATGAAACCACCGGGTTACTGTTCCACCAGTACGGTCCTCATATCTTTCATACTGATAATCAGAAAGTAATGGATTTTATCCAACCCTTTTTTGAACTAAACAATTACCAGCACCGTGTTGGCTTACAACTGGACAACAATTTGGATTTAACGCTTCCATTTGACTTTAGCCAAATGCGCAAGTTGTTGGATACAAAAACAGCTAGCAGTTTAATTAACTTCTTTCAACAGCACTTTCCAGCTGAAAAGCACTTAACACTGATGCAGTTACAAACAATTAACTTTGCTCCAGTCCAACAGCTGTACCAGTTTTTAAAAACAAAGGTGTACGGTCCTTACAGTGTGAAAATGTGGGGCATGCCATTAGAACAAATTGATCCTAGTGTATTGGGTCGAGTCAAAATTAGTTTAAGTGAAAACAGTAGCTATTTCCCTACAGCCACTATTCAAGGTCTACCTAAGGGTGGTTATACAAAGGCCTTTACAAAGATGGTGGATCATCCTCTGATTGATTTAAGGTTAAACTGTCCAGCTAACTTAATTAGCGTAAACAATAATCAACTCTTGTTTGCAAATCAACCAATTACCAAACCAGTGGTTTATTGTGGTTTAATTGATCAGCTGTTTGGCTATTGTTTTGGGCGATTGCAGTACCGCTCGCTCCACTTTGAATGA", "aa": "MDETTGLLFHQYGPHIFHTDNQKVMDFIQPFFELNNYQHRVGLQLDNNLDLTLPFDFSQMRKLLDTKTASSLINFFQQHFPAEKHLTLMQLQTINFAPVQQLYQFLKTKVYGPYSVKMWGMPLEQIDPSVLGRVKISLSENSSYFPTATIQGLPKGGYTKAFTKMVDHPLIDLRLNCPANLISVNNNQLLFANQPITKPVVYCGLIDQLFGYCFGRLQYRSLHFE", "aa_hexdigest": "0c698a9a94ff84fe7a9a69d7797f6445", "start_type": "TTG", "rbs_motif": null, "genes": [ "glf" ], "truncated": "both", "pseudogene": { "start": 326568, "stop": 327716, "observations": { "mutation": [ 326601, 327411 ], "stop": [ 326601, 327411 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75499", "query_cov": 1.0, "subject_cov": 0.5639097744360902, "identity": 0.991, "score": 463.0, "evalue": 1.2e-162, "gene_start": 1, "gene_end": 225, "reference_start": 57, "reference_end": 281, "reference_sequence": "MTLFSFPNYVNWNKFDFIVLGAGISGIVLSHVLAQHGKSVLLLEKRNQLGGNCYDKLDETTGLLFHQYGPHIFHTDNQKVMDFIQPFFELNNYQHRVGLQLDNNLDLTLPFDFSQMRKLLDTKTASSLINFFQQHFPAEKHLTLMQLQTINFAPVQQLYQFLKIKVYGPYSVKMWGMPLEQIDPSVLGRVKISLSENSSYFPTATIQGLPKGGYTKAFTKMVDHPLIDLRLNCPANLISVNNNQLLFANQPITKPVVYCGLIDQLFGYCFGRLQYRSLHFEWKRYAVKQHQAYPVMNWPLHPTITRQVEYKQLTQEGLESNQTIVSCETPGAFREGDPRFMEPYYPLNDVSNNALFARYLKLANAIPNIHLLGRLALYQYIDMDRAIAQSLAKAEQLLQ" }, "paralog": false, "identity": 0.9895287958115183, "score": 776.0, "evalue": 3e-288, "description": "Internal stop codon at 326601, 327411. Nonsense mutation around 326601, 327411.", "psc": { "uniref90_id": "UniRef90_P75499", "gene": "glf", "product": "UDP-galactopyranose mutase", "ec_ids": [ "5.4.99.9" ], "uniref50_id": "UniRef50_P75499", "cog_id": "COG0562", "cog_category": "M", "kegg_orthology_id": "K01854", "go_ids": [ "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75499", "EC:5.4.99.9", "UniRef:UniRef50_P75499", "COG:COG0562", "COG:M", "KEGG:K01854", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75499" ], "product": "UDP-galactopyranose mutase" } }, "id": "GIHJHMOALN_618", "locus": "GCA009939745_00583" }, { "type": "cds", "sequence": "contig_1", "start": 327453, "stop": 327767, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTGGCCACTCCACCCAACAATAACGCGCCAAGTGGAATACAAACAGTTAACGCAAGAGGGTTTGGAGTCTAACCAAACGATTGTTTCGTGTGAAACTCCTGGTGCTTTTAGGGAAGGCGATCCCCGCTTTATGGAGCCGTACTACCCGCTAAATGATGTAAGTAATAATGCCTTGTTTGCGCGTTATTTAAAGTTAGCTAACGCTATTCCTAACATACATTTGTTAGGTCGGTTGGCACTGTACCAGTACATTGATATGGATAGAGCAATTGCTCAAAGCTTAGCTAAAGCCGAACAACTATTACAGTAA", "aa": "MNWPLHPTITRQVEYKQLTQEGLESNQTIVSCETPGAFREGDPRFMEPYYPLNDVSNNALFARYLKLANAIPNIHLLGRLALYQYIDMDRAIAQSLAKAEQLLQ", "aa_hexdigest": "85fcaea78d001ecb592da9f15d933603", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11938.490099999986, "isoelectric_point": 5.580077171325683 }, "id": "GIHJHMOALN_619", "locus": "GCA009939745_00584" }, { "type": "cds", "sequence": "contig_1", "start": 327767, "stop": 329563, "strand": "+", "frame": 2, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "RefSeq:WP_161611425.1", "SO:0001217", "UniParc:UPI00137615DA", "UniRef:UniRef100_UPI00137615DA", "UniRef:UniRef50_B1X3K4", "UniRef:UniRef90_P75498" ], "nt": "ATGGAACAACAAAAAATTAGGAACTTTTCAATTATTGCCCACATTGACCATGGTAAGTCAACGCTGTCAGATCGCTTAATTGAACGCAGCATTGGCTTTGAAAAACGCTTACTGCAAGCGCAAATGTTGGACACCATGGCGATTGAGCGTGAACGGGGTATTACTATTAAACTCAACGCCGTGCAGCTCAAAATGGCCCAAGGCAACCAGCAGTACTTGTTTCACCTAGTTGATACTCCGGGACACGTTGACTTTACTTACGAGGTATCACGCTCTTTAGCAGCTTGTGAGGGTGTTTTACTGTTAGTTGATGCTACCCAAGGGATTCAAGCACAAACGATTTCCAACACTTACTTAGCATTGGAAAACAACCTCGAAATTATTCCGGTAATTAACAAGGTGGACATGGAGAGTGCTGATGTCGAGAAAACCAAACAAGCCTTTCACCAACTGCTAGGGGTGGATCCCAACACCATCCCCTTAGTGTCCGCTAAAACCGGGCTTGGTATTGACCGGTTAATCACTACCATTATTGAAAAAGTACCCCCTCCAAAGGGCGATGAAAGTAAACCACTCAAAGCCTTACTGTTTGACAGCTATTACGATCCTTATAAGGGGGTAGTGTGCTTTATTCGAATCTTTGAGGGGAGTTTAAAACTCAATGATAAGATCCGCTTTGCTAGAAGTAACTCGGTTTACCAAATTGTGGAATTGGGAATTAAAAACCCCTTTTTTGAAAAACAAGACGTGTTAAAAGCCGGGGAGATTGGTTGGTTTTCCGCTGGGATTAAAAAACTAAGGGATGTAACGGTTGGTGACACGATTGTTCACGCTGAGGACACGACCACTCCACCCTTACCGGGTTACAAAAAGGTGTTACCGATGATTTACTGTGGTCTGTACCCGATTGACAACAATGATTACCAAAACCTCAAAATGGCCATGGAAAAGATTATTTTGAGCGATGCGGCCTTGGAGTATGAATATGAAACATCACAAGCATTGGGCTTTGGGGTGCGTTGTGGTTTTTTGGGGTTGTTGCACATGGATGTAATTAAGGAGCGGTTGGAGCGCGAGTACAACCTTAAGTTAATTTCCGCTCCTCCTTCAGTGCGGTATAAGGTGTTGTTAACCAACGGGGAGGAATTGGAACTCGATAACCCTTCACTCTTACCAGAGCGCTCGCGAATTAAATCAATTAGCGAACCGTTTGTACGCGTTTACATTGATCTACCCGATCATTATTTAGGTACAGTAATTGATCTGTGTCAAAACTTCCGTGGGCAATACGAAAAGTTAGAGGAAATTGACATTGACCGCAAACGCTTGGTCTACTTAATGCCCTTGGGTGAAATTATCTACAGTTTCTTTGATAAGTTGAAATCAATCACTAAGGGTTATGCTTCGCTCAATTACGAGTTTGACCAGTACCAGGTGTCCCAATTGGCTAAGGTGGAAATTATGTTAAACAAACAGAAGGTGGACGCTTTATCTTTTATTGCCCACCATGACTTTGCCTTTCAACGGGCCAAGAAGTTCTGTGTCAAATTAAAGGAACTAATTCCCAAGCACCTGTTTGAAATTCCAATTCAAGCCACAATTGGAAGTAAGGCAATTGCTAGAGAAACGATTAAAGCAGTGCGTAAAGATGTAACAGCAAAGCTGTATGGCGGGGATGTCACGCGGAAAAAGAAGCTGTTGGAAAAACAAAAGGAAGGGAAGAAAAGACTGAAAGCAATTGGTAGTGTGGAGCTCCCCCAAGAGCTGTTTAGTCATTTGCTCAAAGATGAAGATTAA", "aa": "MEQQKIRNFSIIAHIDHGKSTLSDRLIERSIGFEKRLLQAQMLDTMAIERERGITIKLNAVQLKMAQGNQQYLFHLVDTPGHVDFTYEVSRSLAACEGVLLLVDATQGIQAQTISNTYLALENNLEIIPVINKVDMESADVEKTKQAFHQLLGVDPNTIPLVSAKTGLGIDRLITTIIEKVPPPKGDESKPLKALLFDSYYDPYKGVVCFIRIFEGSLKLNDKIRFARSNSVYQIVELGIKNPFFEKQDVLKAGEIGWFSAGIKKLRDVTVGDTIVHAEDTTTPPLPGYKKVLPMIYCGLYPIDNNDYQNLKMAMEKIILSDAALEYEYETSQALGFGVRCGFLGLLHMDVIKERLEREYNLKLISAPPSVRYKVLLTNGEELELDNPSLLPERSRIKSISEPFVRVYIDLPDHYLGTVIDLCQNFRGQYEKLEEIDIDRKRLVYLMPLGEIIYSFFDKLKSITKGYASLNYEFDQYQVSQLAKVEIMLNKQKVDALSFIAHHDFAFQRAKKFCVKLKELIPKHLFEIPIQATIGSKAIARETIKAVRKDVTAKLYGGDVTRKKKLLEKQKEGKKRLKAIGSVELPQELFSHLLKDED", "aa_hexdigest": "9d33a8d22f65342a37c4c471ecb4f8f6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00137615DA", "ncbi_nrp_id": "WP_161611425.1", "uniref100_id": "UniRef100_UPI00137615DA", "db_xrefs": [ "SO:0001217", "UniParc:UPI00137615DA", "RefSeq:WP_161611425.1", "UniRef:UniRef100_UPI00137615DA" ] }, "ips": { "uniref100_id": "UniRef100_UPI00137615DA", "uniref90_id": "UniRef90_P75498" }, "psc": { "uniref90_id": "UniRef90_P75498", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_B1X3K4", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "genes": [ "lepA" ], "id": "GIHJHMOALN_620", "locus": "GCA009939745_00585" }, { "type": "cds", "sequence": "contig_1", "start": 329642, "stop": 331345, "strand": "+", "frame": 2, "gene": "rnj", "product": "Ribonuclease J", "db_xrefs": [ "COG:COG0595", "COG:J", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0004534", "GO:0005737", "GO:0006364", "GO:0008270", "SO:0001217", "UniRef:UniRef50_P75497", "UniRef:UniRef90_P75497" ], "nt": "ATGATTAAGGATTTTGATCCTAGTGAGTTTGTCGGTAAAACTCCTACCAAGATTTTTGCCTTTGGTGGTATCCAAGAAGTTGGAAAAAACATGTATGGAATCGAGTACGATGATGAGATTATCATCATCGACTGCGGTATTAAGTTTGCTAGTGATGATCTACTAGGGATTGATGGTATTATCCCGAGCTTCGAATACCTCATTGAAAACCAAGCTAAGGTTAAAGCACTGTTTATCACCCACGGCCATGAAGACCACATTGGTGGGGTGCCTTACTTGTTAAAACAAGTCGATGTACCGGTGATTTACGCACCCCGGATTGCCGCTTCTTTGATCTTAAAGAAGGTCAACGAACACAAGGATGCTAAGCTCAATAAAGTGGTCGTATATGATGACTTTAGCAACTTTGAAACCAAGCACTTCAAGATTGACTTTTACCGCGTCAACCACTCGATTCCTGACGCCTTTGGTGTCTGTGTTCAAACTCCCAACGGTAACATTGTTGAGAGTGGTGACTTTCGCTTTGACTTTGCCGCTGGCGGGGAAATGCTCGATGTGCATAAGGTCGTGAAGATTGCCGAGCGCAATGTCCATGTCTTTATGTGTGAGACTACTAACGCCGAAATCCCGGGGTTCTCCCAGAGCGAAAAGTTAATTTACCGCAACATCAACAAGATTATTAAAGAAGCACGTGGTCGGGTCATTCTCACTACCTTTGCTTCCAACATTACCCGGATTAACGAAATTATTGAGATTGCTGTTAACAATAAACGGAAGGTCTGCTTGTTGGGTAAGTCGATGGATGTGAACGTCAACATCTCGCGTAAGATTGGCTTAATGGACATTGACAGCAATGACATTGTGGAAGTCCGTGACATTAAGAATTACCCCGACCGCAGTATCTTAATCTTGTGTACGGGTTCCCAAGGTGAAGACTCAGCAGCCTTAAACACAATGGCACGGGGTAAACATAACTGGGTTAGCTTAAAGTCCACTGACACCATTATTATGTCCTCCAACCCGATTCCGGGGAACTATGCAGCGGTGGAAAATCTCTTGAATGAGCTTTCGAAGTACGGGGTGACCATCTTTGAAAACTCACCGAACATGAAGCTACACGCCTCTGGTCACGCTACCCAGCAAGAGTTGCAGTTAATGTTAAACCTCGTGTTTCCAAGGTACTTAATTCCGATTCACGGGGAGTACAAGATGATGCGCACCATCAAAAACATAGCGCAAGAATGTGGCATTAACGGTGATGATGTTGGTTTGCTAGCTAACGGTCAGGTGATGTACCTAATTGATGGCAAGCTGTATTACTCAGGGGAAGTGATTAATGCTGACCCGATTTACATTGAAAGCCGCAATTCTTCTCCAGACTTAGCGCGGGTAATTAAACAACGCCAAATCTTGAGTCGGGAGGGAATGTTTGCTGTGATTGTAGTCTTTGACAAAAACAATAACATTTTAGGGATGCCTACTCTGATTACTCGGGGTTGTTTCTTTGCTCTAGATTCCAGTCCGTTAATGACTAAGATTACCCACTCAATTAAACGCGGTTTGGAAAACGTTATTCAAAACAAGCGTTTTAATACCCGTGAACAGATGATCAAGGAATTGAAACGAGTATGTAAGGAAACGGTGTCTTACTTCATTTGGAAGAACAAGAGTCGTAACCCGTTGATTTCAACCGTATTGTCTTGA", "aa": "MIKDFDPSEFVGKTPTKIFAFGGIQEVGKNMYGIEYDDEIIIIDCGIKFASDDLLGIDGIIPSFEYLIENQAKVKALFITHGHEDHIGGVPYLLKQVDVPVIYAPRIAASLILKKVNEHKDAKLNKVVVYDDFSNFETKHFKIDFYRVNHSIPDAFGVCVQTPNGNIVESGDFRFDFAAGGEMLDVHKVVKIAERNVHVFMCETTNAEIPGFSQSEKLIYRNINKIIKEARGRVILTTFASNITRINEIIEIAVNNKRKVCLLGKSMDVNVNISRKIGLMDIDSNDIVEVRDIKNYPDRSILILCTGSQGEDSAALNTMARGKHNWVSLKSTDTIIMSSNPIPGNYAAVENLLNELSKYGVTIFENSPNMKLHASGHATQQELQLMLNLVFPRYLIPIHGEYKMMRTIKNIAQECGINGDDVGLLANGQVMYLIDGKLYYSGEVINADPIYIESRNSSPDLARVIKQRQILSREGMFAVIVVFDKNNNILGMPTLITRGCFFALDSSPLMTKITHSIKRGLENVIQNKRFNTREQMIKELKRVCKETVSYFIWKNKSRNPLISTVLS", "aa_hexdigest": "d21bd145e41819c6527a6f3dc701c2a8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75497", "query_cov": 1.0, "subject_cov": 0.9964850615114236, "identity": 1.0, "score": 1127.0, "evalue": 0.0, "valid": true, "gene": "rnj", "product": "Ribonuclease J", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75497", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0004534", "GO:0003723", "GO:0004521", "GO:0008270", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_P75497", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75497" ], "product": "Ribonuclease J" }, "genes": [ "rnj" ], "id": "GIHJHMOALN_621", "locus": "GCA009939745_00586" }, { "type": "cds", "sequence": "contig_1", "start": 331562, "stop": 332191, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75496", "UniRef:UniRef90_P75496" ], "nt": "ATGAAATTTAAGTATGGAACGGTTGTTTTGGGCAGTTTTTTAGGGCTTTCTGTGGTGTTAGCTGCCTGTGGTGCTCGTGGTAAATTTGACCAATTTGATGATGGCAAAATTAAGCTAGCATCATCCTTAACTTCCAAAGCAGCTGCCAATGCTTTACAAACAATAATAGAGAAGTACAACATTGTCAAAAGCGGCAAGGATTACCCCATTGAAATTACCCAAATTGCTGGTGGTTATGACGGCGGGCGGACTGATCTCCAAACCCGAGTTAGTGTTAAGGATAAAACGAACTTTTATAACCTTATTTTGAACTATCCAGATTTAGTTTCTGTGTTAGCGCGCAGTGGAATGGAACTGCTTTTTGACAAAGTTAATGTTGATAAATTAGAGCCGAACTTCTTAAAGTTTAATGAACAGATTAGTGGAGTCGCTAAAAGTGGTAACTATGGTATTCCCGTGTCCCTTTCAACTGACATTTTGGTCTTAAATGGTCCGGTGTTGCACTATATTTTGAACAGTGCGAAGAAAGAAGAGGCTAATTCTCAAGTTAAAAGTCAAATAAAAACATCACAAATTCAAGCTAAAGGTTCATTAACAATTGATACAGATGAAAATACCAAATCACTTTGA", "aa": "MKFKYGTVVLGSFLGLSVVLAACGARGKFDQFDDGKIKLASSLTSKAAANALQTIIEKYNIVKSGKDYPIEITQIAGGYDGGRTDLQTRVSVKDKTNFYNLILNYPDLVSVLARSGMELLFDKVNVDKLEPNFLKFNEQISGVAKSGNYGIPVSLSTDILVLNGPVLHYILNSAKKEEANSQVKSQIKTSQIQAKGSLTIDTDENTKSL", "aa_hexdigest": "c1111ab55381545f965bdb3047286aef", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 331562, "stop": 332494, "observations": { "mutation": [ 332189, 332360 ], "stop": [ 332189, 332360 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75496", "query_cov": 1.0, "subject_cov": 0.5543766578249337, "identity": 1.0, "score": 401.0, "evalue": 9.03e-139, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MKFKYGTVVLGSFLGLSVVLAACGARGKFDQFDDGKIKLASSLTSKAAANALQTIIEKYNIVKSGKDYPIEITQIAGGYDGGRTDLQTRVSVKDKTNFYNLILNYPDLVSVLARSGMELLFDKVNVDKLEPNFLKFNEQISGVAKSGNYGIPVSLSTDILVLNGPVLHYILNSAKKEEANSQVKSQIKTSQIQAKGSLTIDTDENTKSLWEKIQNSAKANGETNQKGRKAAKSNKTALVQLKNGADTTTNEENKDTKTSDDKVKQTWGDYVEKDDGLKNYTFRASVFENWHDFLDFSTRVAKSFTEKVSNITNKKGTDIQGVLGVDSSPNVLFASVFAAGGGNYENFFYKLKDGRADFSNFKNKGSSYQNLQSVFKD" }, "paralog": false, "identity": 0.9935483870967742, "score": 599.0, "evalue": 4.03e-219, "description": "Internal stop codon at 332189, 332360. Nonsense mutation around 332189, 332360.", "psc": { "uniref90_id": "UniRef90_P75496", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75496", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75496", "UniRef:UniRef50_P75496", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75496", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75496" ], "product": "Uncharacterized lipoprotein MPN_281" } }, "id": "GIHJHMOALN_622", "locus": "GCA009939745_00587" }, { "type": "cds", "sequence": "contig_1", "start": 332417, "stop": 332695, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGAAAACTGGCATGATTTCCTCGATTTTTCGACAAGGGTAGCTAAATCTTTTACTGAAAAGGTAAGCAACATTACCAATAAAAAGGGCACTGATATTCAGGGAGTGCTTGGTGTCGACAGTTCTCCTAATGTTTTATTTGCCTCTGTATTTGCTGCAGGTGGTGGTAATTATGAAAACTTCTTTTATAAGGTGAAAGATGGTCGGGCTGATTTTAGTAACTTTAAGAACAAGGGTAGTTCTTACCAAAACCTCCAAAGCGTTTTTAAAGACTAA", "aa": "MFENWHDFLDFSTRVAKSFTEKVSNITNKKGTDIQGVLGVDSSPNVLFASVFAAGGGNYENFFYKVKDGRADFSNFKNKGSSYQNLQSVFKD", "aa_hexdigest": "43fb4cdc938be627dcbc21d2ae921c39", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10307.276099999994, "isoelectric_point": 7.965665245056153 }, "id": "GIHJHMOALN_623", "locus": "GCA009939745_00588" }, { "type": "cds", "sequence": "contig_1", "start": 332840, "stop": 333550, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7P6", "UniRef:UniRef90_A0AB38W7P6" ], "nt": "TTGAAGTTTAGTGACGACAACACCTTCGTTGAATATCCTGATTTTACTCAGGAAATTAAGTCGCCAGACCAACAAAATGGTCAAAATACTGATGACAATAACAGTAAAAAAGAAGAAAACCTTTTAGGTACCTTTGAAGTTAAAGATTACTCGAAATCTAATAAAGGCGGGAAAGGAAAAAGTAAGGGTAAAGGCGGAAGCAGCAGTAATATGAAGACTATTTACATTTACAAAGGCTCTATTCCTGAAGATAAAAAGAAAGGTGATAATGCCATTTTAGTTACTGAGAAAAAAATTATTGATGCTTTAGAAAAAGCTGCTAATTCTAATGGTGAAAGTACACAATCTAATCCAAAAACCATAGCACTTCAAGCAGCTAAAAAAGAAACTAAAAAGAGCAATAAGAAAGAATCAAAAACGATTGGATATACAACTACTGGTTATGTCAATGCTGATGGTAAACATATTTTTGATACAGACAGAATTAACAATGAGAGGTTTGATCGTAAAATTATTATTGGTGCTACTCTAGAAACTTTAGACCAATCTAGTACACTCCAAAGTGGTGAAGCAATTGTCTTACCAGCACCTGGTAAATATAAGAGTAGTGATCAAAAAACGGTTATGACAGCGCAAGGCCCCAAGATAATCGGGATCCATGCTAATGCTAAAGAGAACGAAGAAACACAAAAATTTGTTAATTGGTTTTAA", "aa": "MKFSDDNTFVEYPDFTQEIKSPDQQNGQNTDDNNSKKEENLLGTFEVKDYSKSNKGGKGKSKGKGGSSSNMKTIYIYKGSIPEDKKKGDNAILVTEKKIIDALEKAANSNGESTQSNPKTIALQAAKKETKKSNKKESKTIGYTTTGYVNADGKHIFDTDRINNERFDRKIIIGATLETLDQSSTLQSGEAIVLPAPGKYKSSDQKTVMTAQGPKIIGIHANAKENEETQKFVNWF", "aa_hexdigest": "7b870a354c1bbbfa9ac9f5abfdb7abb5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W7P6", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.992, "score": 442.0, "evalue": 1.34e-156, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AB38W7P6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7P6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7P6" ], "product": "Lipoprotein" }, "genes": [], "id": "GIHJHMOALN_624", "locus": "GCA009939745_00589" }, { "type": "cds", "sequence": "contig_1", "start": 333646, "stop": 333921, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594", "UniRef:UniRef90_A0AAN4XG79" ], "nt": "TTGCCCTTAAAAGAAAACTTTGAAAAGGTTCAAATTAACGATAAAGGTGAGAAAAATGAAAATGACACAAAGGGCAATACTTATACCGCTAAAGCTTTAGAACTCTTTAAAAAAGTTTCTCAAAATGAAATCGTGAGTTACAGTGACCCAAGTGACTTTAGAAGTGGTAAGTTTCGTGATGCGATTGGCTCTAACTTTAACGCCATTATTAATTCCAAAGGAGACTTCAATAAATTTATTAGTAGTTTTACCGCAAGTTTAGGCTCAGACTTTTAG", "aa": "MPLKENFEKVQINDKGEKNENDTKGNTYTAKALELFKKVSQNEIVSYSDPSDFRSGKFRDAIGSNFNAIINSKGDFNKFISSFTASLGSDF", "aa_hexdigest": "f0d0eb731e90b937640d853f7361250a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 333541, "stop": 333921, "observations": { "mutation": [ 333565 ], "stop": [ 333565 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN4XG79", "query_cov": 1.0, "subject_cov": 0.7222222222222222, "identity": 0.9890000000000001, "score": 175.0, "evalue": 5.05e-55, "gene_start": 1, "gene_end": 91, "reference_start": 36, "reference_end": 126, "reference_sequence": "MVLNTPETWESKRNKKNSNEKQTAAQYFAQEASYILPLKENFEKVQINDKGEKNENDTKGNTYTAKALELFKKVSQNEIVSYSDPSDFRSGKFRDAIGSNFNAIINSKGDFNKFISSFTASLGSDF" }, "paralog": false, "identity": 0.9841269841269841, "score": 246.0, "evalue": 1.27e-85, "description": "Internal stop codon at 333565. Nonsense mutation around 333565.", "psc": { "uniref90_id": "UniRef90_A0AAN4XG79", "product": "Lipoprotein", "uniref50_id": "UniRef50_P75594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN4XG79", "UniRef:UniRef50_P75594" ] }, "pscc": { "uniref50_id": "UniRef50_P75594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75594" ], "product": "Uncharacterized lipoprotein MPN_098" } }, "id": "GIHJHMOALN_625", "locus": "GCA009939745_00590" }, { "type": "cds", "sequence": "contig_1", "start": 334446, "stop": 334763, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6513", "UniRef:UniRef100_A0A0H3DL71", "UniRef:UniRef50_A0A0H3DL71", "UniRef:UniRef90_A0A0H3DL71" ], "nt": "TTGAAGTTATTTTGTCACATTTACTTTTTGGGCAATTTAGATCATGAATACCGAAAAAGTATAAGATTCATTAAGTTAACGAGGTATCACCTCTTTTTAAAACAAGCCCAATCGCAACTCGTTTCTTTGTTTAACCAAAACTTTTTGCAAGTTTTGTTGGCATTTCTAAATAAATCTTTCAATTTATTTAGTGATGACACAAAAATTAATATGTTGAACTTTTTAAATAAATCAACAATGGTATTGATCCCCGTTGGCAGCAGTGCTCCAAGTTCTAGTTCCTTTTTAAATCACCTCAAGAAAGAAGGTATTGCTTAA", "aa": "MKLFCHIYFLGNLDHEYRKSIRFIKLTRYHLFLKQAQSQLVSLFNQNFLQVLLAFLNKSFNLFSDDTKINMLNFLNKSTMVLIPVGSSAPSSSSFLNHLKKEGIA", "aa_hexdigest": "a72389eee8571bf51ad05d84a99f5ac5", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6513", "uniref100_id": "UniRef100_A0A0H3DL71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6513", "UniRef:UniRef100_A0A0H3DL71" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DL71", "uniref90_id": "UniRef90_A0A0H3DL71" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DL71", "uniref50_id": "UniRef50_A0A0H3DL71" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DL71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DL71" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12175.143799999983, "isoelectric_point": 9.868578529357908 }, "id": "GIHJHMOALN_626", "locus": "GCA009939745_00591" }, { "type": "cds", "sequence": "contig_1", "start": 334763, "stop": 334873, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAGAAAATTTCTAAAAAGGAATACAATGCCTTAATCCGTAAAACTGGCGAAAAGCACTTTGACGGCGAAAAGGAAGAGTACGGTGATGGCACCGTTGGACTTTGA", "aa": "MKEKISKKEYNALIRKTGEKHFDGEKEEYGDGTVGL", "aa_hexdigest": "d16543a5863734ff111d693cff88e39d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4129.604900000002, "isoelectric_point": 6.5516260147094725 }, "id": "GIHJHMOALN_627", "locus": "GCA009939745_00592" }, { "type": "cds", "sequence": "contig_1", "start": 334910, "stop": 335110, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAAAGTTAAATACGTAACGCAAGAAGAGTTTGGCGAATTCAAAGATGCCACCAACCAGCGGCTCACAAAGATTGAGAACGCTTTGGTCGCTCAGGGCGAACAAATTAGAGCTCAGGGTGAGCAATTAAGTCAACTAATTAAAGTTGTGCTTCTTCAGGGCAAACAAATAAAATCTCAAGGTGAGCAAATAAAATCTTAG", "aa": "MKVKYVTQEEFGEFKDATNQRLTKIENALVAQGEQIRAQGEQLSQLIKVVLLQGKQIKSQGEQIKS", "aa_hexdigest": "11840b37ae2af7d8cb4eb7115dfd54e0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 64, "aa_cov": 0.9696969696969697, "hmm_cov": 1.0, "evalue": 5.9153044572450576e-15, "score": 51.165557861328125, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 7475.494, "isoelectric_point": 9.099599647521973 }, "id": "GIHJHMOALN_628", "locus": "GCA009939745_00593" }, { "type": "cds", "sequence": "contig_1", "start": 335548, "stop": 335796, "strand": "+", "frame": 1, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "RefSeq:WP_015344899.1", "SO:0001217", "UniParc:UPI0002B8464C", "UniRef:UniRef100_A0AAP8JFL4", "UniRef:UniRef50_A0AAP8E4D4", "UniRef:UniRef90_A0AAP8JFL4" ], "nt": "ATGAAAAAAGTTAAAGTTCTAGAGAAGCCATCTGTTGAAAGAATGATAGAAGTTTTAAGCGACTCTGAAATATTGAAGTTTGATTTTTCTTGCTATTATTTACCTGATGGTACTAATGAGACGCTCATAAGAATCTTTTTTCAAGAAAAAGTTTCAAGTTCTGTAGTAACAAAAGATAAAAATTCTTTAAGACTTGATGAATACTTAAAAGACTTTATTAGCTTCTTAAAAACACAAAGAAAAAACTAA", "aa": "MKKVKVLEKPSVERMIEVLSDSEILKFDFSCYYLPDGTNETLIRIFFQEKVSSSVVTKDKNSLRLDEYLKDFISFLKTQRKN", "aa_hexdigest": "6dad6782f1b05f336bf0127f2ff65eb9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002B8464C", "ncbi_nrp_id": "WP_015344899.1", "uniref100_id": "UniRef100_A0AAP8JFL4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002B8464C", "RefSeq:WP_015344899.1", "UniRef:UniRef100_A0AAP8JFL4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8JFL4", "uniref90_id": "UniRef90_A0AAP8JFL4" }, "psc": { "uniref90_id": "UniRef90_A0AAP8JFL4", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D4" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4" ], "product": "Family K-like protein" }, "genes": [], "id": "GIHJHMOALN_629", "locus": "GCA009939745_00594" }, { "type": "cds", "sequence": "contig_1", "start": 336054, "stop": 336836, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTGAAATATGGAACCATTATTTTCAGTGGTCTTTTGGGAGTTTCCGCCATTTTAGCTGCCTGTGGTGCACGCGGCAAATTTGACCAAATTGATGATGGCAAAATTGTTTTAGCTTCTTCTCTTACATCTAAGGGTGCAGCTAATGCCTTGCAAACAATTGTTAAAAAGTACAACGAAGTTAAAAATATTGATGACTATCCCATTGAAATTATCCAAATTGCCGGTGGTTATGATGGCGGTCGTGGTAACTTACAAACAAAACTAAGTGTTAAAGACAAAAATAGTTTCTATAACTTAATTTTGAACTACCCAGACGTAGTTTCTGTTTTAGGTCGAGTAGGCATGGAATTGCCCTTCGATAAGGTTAGAACAGATAAATTATCACCCCGTTTTCTTGATTTCAACAAGCGAATTAGTGCCATATCTAAGCAAGGTATTTACGGCATTCCAGTCTCTTTGTCTACTGAAGTTTTAGTTTTAAATGGTCCGGTATTGCACTATATTTTGAGCAGTGCAAAGGGTAGTAGTGACAAAACGCAAGTAAGTCAAACATCATCAGGATTAAATCAACAAAAAACTCTTCAAAAACCCTTAAAGATTGATACAAGCGATTCAAGCACTTCTTCTCTTTGGACTCAAATTGAGAACGCCGCTAAAAATAACGGTAAAAAAGCAAATAATAGCAAAAGCAATAGAAGATCCACAGATCAAAGTACACAAACACATAACGATCAAGGCGATGCTTCTGAAAGTGATAAAAAGATTAAAGAATCTTGA", "aa": "MKLKYGTIIFSGLLGVSAILAACGARGKFDQIDDGKIVLASSLTSKGAANALQTIVKKYNEVKNIDDYPIEIIQIAGGYDGGRGNLQTKLSVKDKNSFYNLILNYPDVVSVLGRVGMELPFDKVRTDKLSPRFLDFNKRISAISKQGIYGIPVSLSTEVLVLNGPVLHYILSSAKGSSDKTQVSQTSSGLNQQKTLQKPLKIDTSDSSTSSLWTQIENAAKNNGKKANNSKSNRRSTDQSTQTHNDQGDASESDKKIKES", "aa_hexdigest": "76fb099f4fa871e16b03a4ae902ccf32", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28164.512700000025, "isoelectric_point": 9.487441444396975 }, "id": "GIHJHMOALN_630", "locus": "GCA009939745_00595" }, { "type": "cds", "sequence": "contig_1", "start": 336852, "stop": 338054, "strand": "+", "frame": 3, "gene": null, "product": "P80 family lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001330F80B", "UniRef:UniRef90_UPI001330F80B" ], "nt": "GTGGATGGTGGCTTAAAAGGCTTTACTTTTAAAGCTAGCATCTTTGATAATTGGCACGATTTACTCGATTTTTCTACTAGAGCGGCTAAATCTTTTAAAAAGATAAAAGATAACAATACTAAAAAAGGAACTGATATCCAAGGCATTCTTGGTGTAGATAGTTCAGCCAATTCCTTATTTACTTCTGTTTTTGCAGCTGGTAATGGTGACTATGACAACTTCTTTTACAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAGAGGTAGTTCCTTCCAAAACTTACAAAGTGTTTTTAATGATTACAAGGGTTTAATTGATCAAAACGGTCTCTTTGTTAATAAGGGCGGTTCTTACTCTTCTAACTTTCAAAAGTTTCACCAGTTAGCTTACAGTATCTCCTCTACCTCTGGTTTCTATTATTCATTTGCTGGTAATAGTGCTAAGCGCTTAAAGTTTGGTGATAATTCTTTCATTGAGTATCCACAATATACTGTTCCAATTAAAGCTCCTTCAAAGAATGGTGATGGAAATAGCACAAACAGCAATAGCGATCTTTTAGGCACTTTCACTTTAAGTAGTGGTAAAAAGTCAACAGATAAAAGTAAAAGTGATAGTCAACAAAATCAAGGCAAGAAAGTAGAAGGAACACCTAACCAGGGTAAAAAGGCAGAAGGTGCCCAGAACCAAGGCAAAAAAGAAAATAACAGTACAACTATTGAAATTTACAAGAACAAAATTCCTGATGGTAAAAATGCTGGGAAAGATGCCATCTTAATAAAAGATAACAATCTCATTAAACAGCTAGAAGATGCTGCTAAGAAAAACGGTGCAGAAAGTAATCAAAAACAAGGTGGAGAGAGTAATGGTCAGAAAGAACAAATTATTGGTTATACCACCACTGGTAACGTTCGTGAAGATGGTAATCACATTTTTAGAGTAGACAAAATTAATGACGAACAATACGATCGCAAAATTATTGTTGGCGTTACTGTGGAAACTTTAGAACAGTCTAGCACTTTGCAAAGCGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAATATAAAAGCACTGATAAAAAGAAGGTAACAATTACCCAAGGTCCGAATATTATTGGTATCCATGCTAACGAAAAAGAAAATGCAGAAACCCAAAAGTTTGTTGACTGGTTTTTAAATACAGAAGTAGATTGA", "aa": "MDGGLKGFTFKASIFDNWHDLLDFSTRAAKSFKKIKDNNTKKGTDIQGILGVDSSANSLFTSVFAAGNGDYDNFFYKVANGRADFSNFKNRGSSFQNLQSVFNDYKGLIDQNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFYYSFAGNSAKRLKFGDNSFIEYPQYTVPIKAPSKNGDGNSTNSNSDLLGTFTLSSGKKSTDKSKSDSQQNQGKKVEGTPNQGKKAEGAQNQGKKENNSTTIEIYKNKIPDGKNAGKDAILIKDNNLIKQLEDAAKKNGAESNQKQGGESNGQKEQIIGYTTTGNVREDGNHIFRVDKINDEQYDRKIIVGVTVETLEQSSTLQSEEAIVLAAPGKYKSTDKKKVTITQGPNIIGIHANEKENAETQKFVDWFLNTEVD", "aa_hexdigest": "62672c6de014e2348fc6458f22184733", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001330F80B", "query_cov": 1.0, "subject_cov": 0.8528784648187633, "identity": 0.998, "score": 734.0, "evalue": 1.68e-265, "valid": true, "product": "P80 family lipoprotein", "uniref50_id": "UniRef50_UPI001330F80B" }, "pscc": { "uniref50_id": "UniRef50_UPI001330F80B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001330F80B" ], "product": "P80 family lipoprotein" }, "genes": [], "id": "GIHJHMOALN_631", "locus": "GCA009939745_00596" }, { "type": "cds", "sequence": "contig_1", "start": 338528, "stop": 338707, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGA", "aa": "MEIKTYKIKDICDIKRGRVISKLDIKKDPGVFPVYSAATNNDGEFGRINSYDFDGEYVT", "aa_hexdigest": "6973df725003fab248d0403e95c16b3e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 61, "aa_cov": 0.9661016949152542, "hmm_cov": 0.9836065573770492, "evalue": 3.8072953778139694e-11, "score": 38.95970153808594, "start": 3, "stop": 59 } ], "seq_stats": { "molecular_weight": 6735.564300000002, "isoelectric_point": 6.130108070373535 }, "id": "GIHJHMOALN_632", "locus": "GCA009939745_00597" }, { "type": "cds", "sequence": "contig_1", "start": 338688, "stop": 338783, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGA", "aa": "MVNMLLEQLTVMEGLFFIVMVNLASQIFVVF", "aa_hexdigest": "4165f2c157215872847f65036bab0f87", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3577.3857, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_633", "locus": "GCA009939745_00598" }, { "type": "cds", "sequence": "contig_1", "start": 338828, "stop": 339358, "strand": "+", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q50287", "UniRef:UniRef90_UPI0027E16080" ], "nt": "TTGAAACTAGAAGCACCGAAATTCACAAACCGTGTTTTTAAAAATAGACCTAAGTTAACACATAAAACAATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGAGAGCGCAGGAAGCAGTATGACTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAATAAGGGATATTTGTGAAATAAATCGTGGTCGAGAAATAAATGAAAAATACCTTCGAGAAAATCCAGGTGAATTTCCAGTTTATTCCTCAGCAACAACAAATGGTGGTTTAATTGGAAAAATTAATGATTACGATTTTCATGGTGAATATGTTACTTGA", "aa": "MKLEAPKFTNRVFKNRPKLTHKTMAEIPIDFPPLKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELRERRKQYDFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVT", "aa_hexdigest": "ac16bd82d9a47e6541c278cb6597e84e", "start_type": "TTG", "rbs_motif": "AAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 338855, "stop": 339661, "observations": { "mutation": [ 339356 ], "stop": [ 339356 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0027E16080", "query_cov": 0.9943181818181818, "subject_cov": 0.5681818181818182, "identity": 0.9309999999999999, "score": 234.0, "evalue": 2.72e-74, "gene_start": 10, "gene_end": 176, "reference_start": 1, "reference_end": 175, "reference_sequence": "NRVFKNRPKLTHKTMAEIPIDFPPLKIQEKIATILDTFTELSTELSTELSTELSAELSAELSAELSAELSAELSAELSAELSAELSAELRERRKQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVTWTTGGAHAGNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAEIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFNWVQNKKLESLKSL" }, "paralog": false, "identity": 0.9528985507246377, "score": 432.0, "evalue": 1.37e-154, "description": "Internal stop codon at 339356. Nonsense mutation around 339356.", "psc": { "uniref90_id": "UniRef90_UPI0027E16080", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q50287", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0027E16080", "EC:3.1.21.3", "UniRef:UniRef50_Q50287", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_Q50287", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50287" ], "product": "Putative type I specificity subunit S.MpnORF201P" } }, "id": "GIHJHMOALN_634", "locus": "GCA009939745_00599" }, { "type": "cds", "sequence": "contig_1", "start": 339431, "stop": 339718, "strand": "+", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0AB33HM83", "UniRef:UniRef90_UPI0019133825" ], "nt": "TTGTTAGAAGTAAAGAACAAAAATAAATTTAGTTCCAAATTCTTATGTTTTGCACTAAAACTACAATCTAAAAAATTTGTGAATTATGCCTCTGCAATTCCGGTTCTAACAATAAAGAGAATTGCAGAAATCGAACTCTCCTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTTTGTTTGCATTTGAAAAACTTTGCAACGATTTAACCGAGGGGATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGA", "aa": "MLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAEIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "a11db2ee396e898e6d3a89d9fdf70479", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 339131, "stop": 339727, "observations": { "mutation": [ 339356, 339716 ], "stop": [ 339356, 339716 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019133825", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 0.9890000000000001, "score": 184.0, "evalue": 1.83e-57, "gene_start": 1, "gene_end": 95, "reference_start": 108, "reference_end": 202, "reference_sequence": "KQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVTWTTGGAHAGNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAEIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFNWVQKIRN" }, "paralog": false, "identity": 0.98989898989899, "score": 397.0, "evalue": 9.01e-144, "description": "Internal stop codon at 339356, 339716. Nonsense mutation around 339356, 339716.", "psc": { "uniref90_id": "UniRef90_UPI0019133825", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AB33HM83", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019133825", "EC:3.1.21.3", "UniRef:UniRef50_A0AB33HM83", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HM83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HM83" ] } }, "id": "GIHJHMOALN_635", "locus": "GCA009939745_00600" }, { "type": "cds", "sequence": "contig_1", "start": 339767, "stop": 340105, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ], "nt": "TTGGTCGAGGGTGGGTCATTTGGGAATATCTTTCCCCGTAGTCGTCGTCTGACTGCCACCCAGGGTGCCCTGGACATTCGCTTTGAGGCCAACGCCGACATTCGAAAAAGTGGGTGCGGGTTCGGTTCCGGATGCATTTTGCGATTGTTGTCCCTGGGTTGTTACCCCACTCGTACTACTGTTGCTCCCCCGGTGGTCATTCCCATACCTGTGCCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCGAGAACCGACCCCGCGAGGTTGAACCCGGTGGAGAAGCGCGTGAGCGTGGGGGAGAAGTGGGATCAGTTTGTGGTTTGCTGAACTAA", "aa": "MVEGGSFGNIFPRSRRLTATQGALDIRFEANADIRKSGCGFGSGCILRLLSLGCYPTRTTVAPPVVIPIPVPIPNPGNIIQHLVENRPREVEPGGEARERGGEVGSVCGLLN", "aa_hexdigest": "a6dda59632b6d32ed353fd37be435579", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "query_cov": 1.0357142857142858, "subject_cov": 0.8226950354609929, "identity": 0.586, "score": 107.0, "evalue": 1.54e-27, "valid": false, "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11865.52089999999, "isoelectric_point": 8.504556465148926 }, "id": "GIHJHMOALN_636", "locus": "GCA009939745_00601" }, { "type": "cds", "sequence": "contig_1", "start": 340463, "stop": 340789, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTGAATAAGAGTGGCCAAGATGATTCCAACAAGTTCCAAGCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAGGTGTACGGTCTGAGTTCCACTCAAGATTCTAGCACCGGAGCCGGTGGCGCTGGAGGTAACACCGGAGGCGACACCAATACCCAGACTTATGCACGACCCGGAATCGGCTTTAAATTACCCTCCACGGACTCGGAATCTTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNKSGQDDSNKFQATDQKWSYTELKSDQTKLNLPAYGEVNGLLNPALVEVYGLSSTQDSSTGAGGAGGNTGGDTNTQTYARPGIGFKLPSTDSESSKATLITPGLA", "aa_hexdigest": "3599a4e0ed2bff56344026874b743ef7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11204.079199999984, "isoelectric_point": 4.654454231262207 }, "id": "GIHJHMOALN_637", "locus": "GCA009939745_00602" }, { "type": "cds", "sequence": "contig_1", "start": 341022, "stop": 341243, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03257.18" ], "nt": "GTGTGGGATTTCAAGGGGGTGTGGCAGGATCAAGCTCGGTCCGACTCGCAAGCAGCTACAAGTACCACCACCGCAACGAAGGCTACCTTATCGGAGCACACCAATGCTTTGGCCTTTCAGGTGAGTTATACCGACCAGGATTCGTACAAGGCTTCCACTCAAAGCTCCGGCCAAAACCAAAACACCTCCCCCTACCTGCACTTGGTGCAGGGAAAAAAGTAG", "aa": "MWDFKGVWQDQARSDSQAATSTTTATKATLSEHTNALAFQVSYTDQDSYKASTQSSGQNQNTSPYLHLVQGKK", "aa_hexdigest": "94616c3ebb89eb4b96ee75d1db6d8df7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 31, "aa_cov": 0.3972602739726027, "hmm_cov": 1.0, "evalue": 3.291176109014823e-10, "score": 35.97065734863281, "start": 45, "stop": 73 } ], "seq_stats": { "molecular_weight": 8045.573400000003, "isoelectric_point": 6.699748420715332 }, "id": "GIHJHMOALN_638", "locus": "GCA009939745_00603" }, { "type": "cds", "sequence": "contig_1", "start": 341282, "stop": 341500, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03257.18" ], "nt": "TTGGACCCCAACGAGGTTCGCGCCAAGCTGCGCCAAAGCTTTGGTACAGAAAATTCTACCCAACCCCAACCTAAAGCCCTCAAAACAACCACACCGGTATTTGGGACGAATAGTGGTAACCTCGGCAGTGTGCTTGGTGGTGGGGGTACCACGCAGGGCTCAGGCTCAGGTCAATCTGGTGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAA", "aa": "MDPNEVRAKLRQSFGTENSTQPQPKALKTTTPVFGTNSGNLGSVLGGGGTTQGSGSGQSGVDLSPVERVSGH", "aa_hexdigest": "5a981fa84af8ad3f9787c4ddc4f4a68c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 72, "aa_cov": 1.0, "hmm_cov": 0.9861111111111112, "evalue": 6.312775026603139e-27, "score": 89.86980438232422, "start": 1, "stop": 72 } ], "seq_stats": { "molecular_weight": 7270.845000000002, "isoelectric_point": 8.275049018859864 }, "id": "GIHJHMOALN_639", "locus": "GCA009939745_00604" }, { "type": "cds", "sequence": "contig_1", "start": 341819, "stop": 342343, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_287", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D866ED", "UniRef:UniRef100_P75490", "UniRef:UniRef50_P75490", "UniRef:UniRef90_P75490" ], "nt": "TTGCTGCCCACCTCTTTAAACACTTTTACGTTTTTCCAATTAATAATAAAGGAGGTCTTCACCATTAAACGCACAATTTCACTTACTTTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTATGACATGGGATTTAGTGACAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAATAATCAAAGGCTGATAAAGATTGAAACTACTTTAGCTACTCAAGGTGAACAATTAAATCAATTAATTAAAGTTGTTATTCTTCAGGGCGAACAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAAGGAGAACAAATAAAAGCTAAAGGAGAACAAATAAAAGCTCAAAGCGAGCAAATAAAAACTCAAGGAGAAACGCTTAAATTGATCCTACAAGCACTTGGAGGAATAAATAAGCGCTTAGACAAAGTTGATCCGCCTAAATAG", "aa": "MLPTSLNTFTFFQLIIKEVFTIKRTISLTLFKKRLNKDKINNCHVWEEELPDGSYDMGFSDNLNHMEKRKSGYVTQKQFSEFKDANNQRLIKIETTLATQGEQLNQLIKVVILQGEQIKELQVEQKAQGEQIKAKGEQIKAQSEQIKTQGETLKLILQALGGINKRLDKVDPPK", "aa_hexdigest": "4f27146806afe1ad4690dfc75aa0b72c", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002D866ED", "uniref100_id": "UniRef100_P75490", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D866ED", "UniRef:UniRef100_P75490" ] }, "ips": { "uniref100_id": "UniRef100_P75490", "uniref90_id": "UniRef90_P75490" }, "psc": { "uniref90_id": "UniRef90_P75490", "product": "UPF0134 protein MPN_287", "uniref50_id": "UniRef50_P75490" }, "pscc": { "uniref50_id": "UniRef50_P75490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75490" ], "product": "UPF0134 protein MPN_287" }, "genes": [], "id": "GIHJHMOALN_640", "locus": "GCA009939745_00605" }, { "type": "cds", "sequence": "contig_1", "start": 342940, "stop": 343581, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75595", "UniRef:UniRef90_A0AB38WA11" ], "nt": "ATGAAATTTAAGTATTGTGCCATCTTTTTCAGTGGCTTTCTAGGTCTTTCTGCCATCTTAGCTGCTTGTGGAGCGCGGGGCAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTTCTCTTTAACTTCAAAAAGTGCATCAAACGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCATATTGAAATTACCCAAATCGCTGGTGGTTATGATGGTGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAGGACACCACTAACTTTTACAACCTTATCTTGAATTATCCTGACTTAGTTTCTACTTTAGGTCGTGTTGGGATGGAACTACTGTTTGATAAAGTTAATACAGATAAATTATCCGACCGTTTCTTAGATTTCAATAAACGAATTAGTGCAATTTCTAAACCAGGGATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAAAAGTGCAAAAGGTGATAGTGACAATGTTAAAGTAAGTCAAAGGACTGGAGAAACTACTCAAAAAAGTAAAGTAACTAATCCTTTGAAAATTAATACTCAAAACGATCCTGCTACAAAAGATCTTTGA", "aa": "MKFKYCAIFFSGFLGLSAILAACGARGKFDQVDDGKIKLAFSLTSKSASNALQAIVKKYNEVKKPGDYHIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDL", "aa_hexdigest": "43c47b40f91aac03cb97426358e1305f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 342940, "stop": 343884, "observations": { "mutation": [ 343579, 343759, 343828 ], "stop": [ 343579, 343759, 343828 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38WA11", "query_cov": 1.0, "subject_cov": 0.44375, "identity": 1.0, "score": 415.0, "evalue": 1.13e-142, "gene_start": 1, "gene_end": 213, "reference_start": 1, "reference_end": 213, "reference_sequence": "MKFKYCAIFFSGFLGLSAILAACGARGKFDQVDDGKIKLAFSLTSKSASNALQAIVKKYNEVKKPGDYHIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDLWEKIEASAKANGKSNKDGQTKGKKKVEKSSSSSLVNLKQSTDQTTTDDGSQKSDNKIKESWGEYQEVDGGLKNFEFKASIFENWHDLLDFSTRVAKSFTNVKGKDIKKGTDIQGVLGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFGDNFIEFPRFTQEIKAPSTENGGQSNLLGTFEVKDSSKSKEVKKN" }, "paralog": false, "identity": 0.9904458598726115, "score": 606.0, "evalue": 5.13e-220, "description": "Internal stop codon at 343579, 343759, 343828. Nonsense mutation around 343579, 343759, 343828.", "psc": { "uniref90_id": "UniRef90_A0AB38WA11", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75595", "go_ids": [ "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38WA11", "UniRef:UniRef50_P75595", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75595" ], "product": "Uncharacterized lipoprotein MPN_097" } }, "id": "GIHJHMOALN_641", "locus": "GCA009939745_00606" }, { "type": "cds", "sequence": "contig_1", "start": 343858, "stop": 344940, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P75489" ], "nt": "GTGGCTAAGTCGTTTACAAATGTAAAGGGAAAAGATATCAAAAAGGGAACTGATATCCAAGGTGTACTTGGTGTTGACAGTACGCCCAACTCATTATTCACCTCTGTTTTTGCAGCTGGTGGCGGTGATTACAATAATTTCTTCTACAAAATTGAAAACGGCCGTGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCCTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTTCAAAAGTTCCATCAATTAGCTTACAGTATTTCCTCTACTTCCGGTTTTTTCTATTCGTTTGCCGGTAACAGCGCTAAACGCTTAAAGTTTGGTGATAACTTTATTGAATTTCCTCGTTTTACTCAGGAAATTAAAGCACCTTCTACTGAAAATGGCGGCCAAAGCAATCTTTTAGGAACCTTTGAAGTTAAAGATAGCAGTAAATCTAAGGAAGTGAAAAAAACTAATAGGAAAGAAGATGGTGCTCAAAATCAAGGTAAAAAAGAAAGCGATAAGAAAACCATTTACCTTTACAAAAGTCAAATTCCATCAGACAAAACAGAAGGCGAAAATGCCATCTTAATCAAAGAACAAAACGTTATTAATCAGCTAGAACAAGCAGCTAAAAAAGATGAAAAAGGAGAGACTGTTACAAACAAAGTTGCATCATTAGAAACCAAAGCTGCTAATGCCAAAAAGGATAGTAGTAAAACCATTATTGGTTACACCACAACTGATAATGTTCATGAAGACGGTAAAAACATTTTCAAAATTAACAAACTCAAAACTGAAGACTATGATCGTAAGATTATTGTCGGTGCCACTGAAGAAGTGTTAGAGCAATCTAGCACATTACAAAGTGATGAAGCAATTGTGTTACCAGCTCCTGGTAAGTATCAAAGTGGTGACGCTAAGAAAGTAACCATCACCCAAGGTCCTAATATCATTGGCATTCACGCTAACGAAAAGGAAAACGCCGAAACGCAAAAGTTTGTTAACTGGTTTTTAAACAATACAGAAAGTTGA", "aa": "MAKSFTNVKGKDIKKGTDIQGVLGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFGDNFIEFPRFTQEIKAPSTENGGQSNLLGTFEVKDSSKSKEVKKTNRKEDGAQNQGKKESDKKTIYLYKSQIPSDKTEGENAILIKEQNVINQLEQAAKKDEKGETVTNKVASLETKAANAKKDSSKTIIGYTTTDNVHEDGKNIFKINKLKTEDYDRKIIVGATEEVLEQSSTLQSDEAIVLPAPGKYQSGDAKKVTITQGPNIIGIHANEKENAETQKFVNWFLNNTES", "aa_hexdigest": "821b452638e11938494d378b5c1fb560", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 343558, "stop": 345243, "observations": { "mutation": [ 343579, 343759, 343828, 344938 ], "stop": [ 343579, 343759, 343828, 344938 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75489", "query_cov": 1.0, "subject_cov": 0.4235294117647059, "identity": 0.997, "score": 686.0, "evalue": 2.9e-241, "gene_start": 1, "gene_end": 360, "reference_start": 370, "reference_end": 729, "reference_sequence": "MKFKYCAIFFSGFLGLSAILAACGTKGKFDQVDDGKIKLASSLTSKSASKALQAIVKKYNEVKKPGDYPIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKDRFLDFNNRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDLWEKIEASAKANGKSNKDGQTKGKKKVEKSSSSSLVNLKQSTDQTTTDDGSQKSDNKIKESWGEYQEVDGGLKNFEFKASIFENWHDLLDFSTRVAKSFTNVKGKDIKKGTDIQGVLGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFGDNFIEFPRFTQEIKAPSTENGGQSNLLGTFEVKDSSKSKEVKKTNRKEDGAQNQGKKESDKKTIYLYKSQIPSDKTEGENAILIKEQNVINQLEQAAKKDEKGETVTNKVASLETKAANAKKDSSKTIIGYTTTDNVHEDGKNIFKINKLKTEDYDRKIIVGATEEVLEQSSTLQSDEAIVLPAPGKYQSGDAKKVTITQGPNIIGIHANEKENAETQKFVNWFLNNTESWEVKNGKDSSTKQQTAAEFFAESASYILPLKEIFDKDNKKATENKDNNTSNKKQANTYAEKALDLFQQISKGDIVSYSDPSDFRSGKFRDGIGSNFNAAVSSKADFDRFVKGFIATLGSEI" }, "paralog": false, "identity": 0.9928698752228164, "score": 1092.0, "evalue": 0.0, "description": "Internal stop codon at 343579, 343759, 343828, 344938. Nonsense mutation around 343579, 343759, 343828, 344938.", "psc": { "uniref90_id": "UniRef90_P75489", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75489", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "GIHJHMOALN_642", "locus": "GCA009939745_00607" }, { "type": "cds", "sequence": "contig_1", "start": 345019, "stop": 345303, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTAAAAGAAATCTTTGACAAAGATAACAAAAAAGCTACTGAAAATAAGGACAATAATACTAGCAATAAAAAACAAGCTAATACCTATGCTGAAAAGGCGTTAGATTTGTTCCAACAAATTAGTAAAGGTGATATAGTTAGTTACAGCGACCCTAGCGATTTTAGAAGTGGTAAATTCCGCGATGGGATTGGCTCTAATTTTAACGCTGCAGTAAGTTCCAAAGCTGATTTTGACAGGTTTGTTAAAGGCTTCATCGCCACTTTGGGTAGTGAAATTTAA", "aa": "MPLKEIFDKDNKKATENKDNNTSNKKQANTYAEKALDLFQQISKGDIVSYSDPSDFRSGKFRDGIGSNFNAAVSSKADFDRFVKGFIATLGSEI", "aa_hexdigest": "ccda41382d0f2345a6eb88c671150457", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10433.430199999992, "isoelectric_point": 7.908417320251465 }, "id": "GIHJHMOALN_643", "locus": "GCA009939745_00608" }, { "type": "cds", "sequence": "contig_1", "start": 345393, "stop": 345572, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGCAAATCAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAACTATATATCAAAAACAATCCAGGCGAATTCCCTGTTTATTCATCGGCTACAGTTAATAATGGTGAAATTGGCAGAATAAAAGATTGTGATCTTAAGGGTGAGTATGTGACTTGA", "aa": "MQIKTYKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGRIKDCDLKGEYVT", "aa_hexdigest": "586c91d0b4398bcdcba1cdfcc9a437c9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 60, "aa_cov": 0.9491525423728814, "hmm_cov": 0.9833333333333333, "evalue": 7.859326532128574e-11, "score": 37.9355354309082, "start": 4, "stop": 59 } ], "seq_stats": { "molecular_weight": 6724.738800000003, "isoelectric_point": 9.230019187927244 }, "id": "GIHJHMOALN_644", "locus": "GCA009939745_00609" }, { "type": "cds", "sequence": "contig_1", "start": 345750, "stop": 345998, "strand": "+", "frame": 3, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF289P N-terminus", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75488", "UniRef:UniRef90_P75488" ], "nt": "ATGCAAGGAACTTTAGCAGAAATTGAGCTAGATTTCCCAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGTCTTCTATAGCGATTATTTACTTAACCCAAAAAATTGA", "aa": "MQGTLAEIELDFPSKKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELRERKKQYVFYSDYLLNPKN", "aa_hexdigest": "8b753cd7dc4cfd2d39362101878bcc44", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 345450, "stop": 346004, "observations": { "mutation": [ 345570, 345996 ], "stop": [ 345570, 345996 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75488", "query_cov": 1.048780487804878, "subject_cov": 0.41545893719806765, "identity": 0.953, "score": 80.5, "evalue": 5.71e-17, "gene_start": 1, "gene_end": 82, "reference_start": 120, "reference_end": 205, "reference_sequence": "MQIKTYKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGRIKDCDLKGEYVTWTTDGAQAGSVFYRNGQFNATNVCGILKVNNDEIYPKFLAYALRLKAPKFVNYACPIPKLMQGTLAEIELDFPSKKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELSAELRERKKQYVFYSDYLLNPKNWK" }, "paralog": false, "identity": 0.9680851063829787, "score": 281.0, "evalue": 2.19e-98, "description": "Internal stop codon at 345570, 345996. Nonsense mutation around 345570, 345996.", "psc": { "uniref90_id": "UniRef90_P75488", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF289P N-terminus", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75488", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75488", "EC:3.1.21.3", "UniRef:UniRef50_P75488", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75488", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75488" ], "product": "Putative type I specificity subunit S.MpnORF289P N-terminus" } }, "id": "GIHJHMOALN_645", "locus": "GCA009939745_00610" }, { "type": "cds", "sequence": "contig_1", "start": 346143, "stop": 346535, "strand": "+", "frame": 3, "gene": null, "product": "Putative type I specificity subunit S-MpnORF289P C-terminus", "db_xrefs": [ "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75487", "UniRef:UniRef90_P75487" ], "nt": "TTGGGTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGTCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTCCCTAAAGAAGAATTTGATATAAGGTTCTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGCTCTGCAACAGGCCAGTTAACAGTGGCACAATTTAAGGAATATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCGCCGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTCTTCCTGCTGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTTTTATTTAACTGA", "aa": "MGYSKTFRVEEKSITVSARGTIGVVFYRDFAYLPAVSLICFVPKEEFDIRFLFHALRAIKFKKQGSATGQLTVAQFKEYGIHVPSLKKQKEIAAILDPLYSFFTDLNEGLPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "f42dc3e4dac22884f74136c0cd6c8b10", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75487", "query_cov": 1.0, "subject_cov": 0.896551724137931, "identity": 0.992, "score": 256.0, "evalue": 3.17e-86, "valid": true, "product": "Putative type I specificity subunit S.MpnORF289P C-terminus", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75487", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75487", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75487" ], "product": "Putative type I specificity subunit S.MpnORF289P C-terminus" }, "genes": [], "id": "GIHJHMOALN_646", "locus": "GCA009939745_00611" }, { "type": "cds", "sequence": "contig_1", "start": 346573, "stop": 347163, "strand": "-", "frame": 1, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "COG:COG1214", "COG:J", "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_014325445.1", "SO:0001217", "UniParc:UPI0001DD651C", "UniRef:UniRef100_A0AAP8E4Y3", "UniRef:UniRef50_P47450", "UniRef:UniRef90_P75486" ], "nt": "ATGCGCTTTTTCAATAAGTACAAGCTCTTTTTGGACTGTGCTTACAAACACCTCAATATCGTACTGTTGGATTTTAAAACGAATACTGTGGTGGATCAACTAACAATACCAGTACAACAAAACCTCACGGAGTTGGCTGTGTATCACTTGGAAAAATTGTTAAAGAAAAACAAAGTACGCAATAACACTGTTAGACAGTTTTACGTAACCACTGGACCGGGTAGCTTTACTGGTCAACGCGTTGGTGCCATTATTGCTAAAACTTGGTGCACAGTAAACCCTAACTGTCAACTCTTTGCTTTAAACTCACTCCGCCTTCAAATTCCTTATGGCTGTGGCATTAGCAAAATTAGTGCTGGCAATGAAAAGAACTACTGTGGTTTGTTTACCGAAACTACGAGTGAAATAGCACTCTTAGCTAAACCAGATTTTGTCAAGCTGTGCAAAGCAAATACCGAGTTACCGTTATATGAAAACTTTGAAAACATCGACAGTATTGAGGAGTTATTCCTAAACAACATCGAACGGTTTGAACTAGTGGAAAATCCCCAAAATTTAGAACTACTCTACCTTAAAGATCCCGTTAATTAG", "aa": "MRFFNKYKLFLDCAYKHLNIVLLDFKTNTVVDQLTIPVQQNLTELAVYHLEKLLKKNKVRNNTVRQFYVTTGPGSFTGQRVGAIIAKTWCTVNPNCQLFALNSLRLQIPYGCGISKISAGNEKNYCGLFTETTSEIALLAKPDFVKLCKANTELPLYENFENIDSIEELFLNNIERFELVENPQNLELLYLKDPVN", "aa_hexdigest": "4637762e47aa82473716d0c7947bb369", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0001DD651C", "ncbi_nrp_id": "WP_014325445.1", "uniref100_id": "UniRef100_A0AAP8E4Y3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD651C", "RefSeq:WP_014325445.1", "UniRef:UniRef100_A0AAP8E4Y3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E4Y3", "uniref90_id": "UniRef90_P75486" }, "psc": { "uniref90_id": "UniRef90_P75486", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P47450", "cog_id": "COG1214", "cog_category": "J", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_P47450", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47450" ], "product": "Uncharacterized protein MG208" }, "genes": [ "tsaB" ], "id": "GIHJHMOALN_647", "locus": "GCA009939745_00612" }, { "type": "cds", "sequence": "contig_1", "start": 347147, "stop": 347497, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGAACAGTTCTAACAAGGCCAAGAATGCTTTTACTAAGTTTACGGTGTTAAACCAAAACGAAAAGGCTGCTTTAATTAAGTGTGAACTGTTAACCGGACGAACTCACCAAATTAGGGTACACTTGCAGTTTATTAAACACCCCGTTTACAACGATCCCTTATACGGTTTGAAGGGTGAGCAAGCTACTGAATATGGCCAGTATTTACACGCGCAACAAATTAGTTTTATCCACCCCACTTTGAATAAGGAAATGGGTTTTGAAGCACAGTTAGATAAAACTTTTAGCGATAAGTTAGATAATTTAAACTTGAAAATTGCTAATTCCCTTTATGCGCTTTTTCAATAA", "aa": "MGNSSNKAKNAFTKFTVLNQNEKAALIKCELLTGRTHQIRVHLQFIKHPVYNDPLYGLKGEQATEYGQYLHAQQISFIHPTLNKEMGFEAQLDKTFSDKLDNLNLKIANSLYALFQ", "aa_hexdigest": "384f69b6fa9ffd4eb57c45f6ee9075d6", "start_type": "GTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13256.989299999987, "isoelectric_point": 9.015726280212402 }, "id": "GIHJHMOALN_648", "locus": "GCA009939745_00613" }, { "type": "cds", "sequence": "contig_1", "start": 347501, "stop": 347926, "strand": "-", "frame": 3, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG209-like protein", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0120159", "KEGG:K06180", "SO:0001217", "UniRef:UniRef50_P47451", "UniRef:UniRef90_P75485" ], "nt": "GTGCAACCAGAAGACCGGGTACACGTTAATTGGAGTGAGGAACTGTTTGAAAAAGTGCCAGTTGAAGTCCAGCCCTATGACTTTCCCTTGGACATCTTATATGAAGATGAGCAAATTATGGTGGTCAATAAACCCAATGGTTTAATTAGCCACCCGACTAGTTTTAATGAAAGTGAAAGTTTACTGGGTGCCGCTTTGTTTCACTGCAACCACCAACCGGTGTTTTTAGTCCACCGCCTAGACCGTGATACGAGTGGTGTGATTATGCTAGCGAAAAACCAAAGTAGCTTGCTGCACTTGCAAAAACAGCTCCAGCAACGGGTTATGAAGCGTTATTACTTAGCTTTAGTCCATTTTCCCCTAGACAGTTTGTCTGGTACCATTAGCGCCCCGTTAGAACGAGTGGGTAACAATAAGGTCATGTGA", "aa": "MQPEDRVHVNWSEELFEKVPVEVQPYDFPLDILYEDEQIMVVNKPNGLISHPTSFNESESLLGAALFHCNHQPVFLVHRLDRDTSGVIMLAKNQSSLLHLQKQLQQRVMKRYYLALVHFPLDSLSGTISAPLERVGNNKVM", "aa_hexdigest": "f4b07f53c49b25a332940f18f3a24ad4", "start_type": "GTG", "rbs_motif": null, "genes": [ "rluA" ], "truncated": "both", "pseudogene": { "start": 347198, "stop": 348076, "observations": { "mutation": [ 347503, 347932 ], "stop": [ 347503, 347932 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75485", "query_cov": 1.0, "subject_cov": 0.45483870967741935, "identity": 0.993, "score": 282.0, "evalue": 5.52e-94, "gene_start": 1, "gene_end": 141, "reference_start": 51, "reference_end": 191, "reference_sequence": "MEQTFSVTTAQRLDTFLATLLNLSRVKVAKLIVDGLVSVNGKKITKNGWLVQPEDRVHVNWSEELFEKVPVEVQPYDFPLDILYEDEQIMVVNKPNGLISHPTSFNESESLLGAALFHCNHQPVFLVHRLDRDTSGVIMLAKNQSSLLHLQKQLQQRVMKRYYLALVHFPLDSLSGTISAPLERVGNNKVMWKVGNSSNKAKNAFTKFTVLNQNEKAALIKXCELLTGRTHQIRVHLQFIKHPVYNDPLYGLKSEQATEYGQYLHAQQISFIHPTLNKEMGFEAQLDKTFSDKLDNLNLKIANSLYALFQ" }, "paralog": false, "identity": 0.9863481228668942, "score": 568.0, "evalue": 3.59e-209, "description": "Internal stop codon at 347503, 347932. Nonsense mutation around 347503, 347932.", "psc": { "uniref90_id": "UniRef90_P75485", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG209 homolog", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47451", "cog_id": "COG0564", "cog_category": "J", "kegg_orthology_id": "K06180", "go_ids": [ "GO:0003723", "GO:0120159", "GO:0000455" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75485", "EC:5.4.99.-", "UniRef:UniRef50_P47451", "COG:COG0564", "COG:J", "KEGG:K06180", "GO:0003723", "GO:0120159", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47451", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47451" ], "product": "Uncharacterized RNA pseudouridine synthase MG209" } }, "id": "GIHJHMOALN_649", "locus": "GCA009939745_00614" }, { "type": "cds", "sequence": "contig_1", "start": 347930, "stop": 348076, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01479.30" ], "nt": "ATGGAACAAACTTTTAGTGTTACTACAGCCCAACGCTTAGATACCTTTTTGGCCACACTACTCAACCTTTCCCGGGTTAAAGTAGCCAAGTTAATTGTCGATGGTTTGGTGAGTGTTAACGGTAAAAAAATTACTAAAAATGGTTGA", "aa": "MEQTFSVTTAQRLDTFLATLLNLSRVKVAKLIVDGLVSVNGKKITKNG", "aa_hexdigest": "611db4e9f4b8d7087542eda5e7a7ab1c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01479.30", "name": "S4", "length": 38, "aa_cov": 0.7708333333333334, "hmm_cov": 1.0, "evalue": 9.300516969376866e-12, "score": 40.05647277832031, "start": 11, "stop": 47 } ], "seq_stats": { "molecular_weight": 5220.094100000002, "isoelectric_point": 10.165906715393067 }, "id": "GIHJHMOALN_650", "locus": "GCA009939745_00615" }, { "type": "cds", "sequence": "contig_1", "start": 348076, "stop": 348177, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAACTTAGCGGACTGTTGCATTACCTTTAGTTTTATTGGTCTGTTCCTCTCCTTTTTAATTCAGTTCTTCAAAGAAATGAAGCAAACTAAATCTTAA", "aa": "MFNLADCCITFSFIGLFLSFLIQFFKEMKQTKS", "aa_hexdigest": "54ad894d5bc253b4bf89f0e5464f84d7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3899.661100000001, "isoelectric_point": 7.799981498718263 }, "id": "GIHJHMOALN_651", "locus": "GCA009939745_00616" }, { "type": "cds", "sequence": "contig_1", "start": 348280, "stop": 348630, "strand": "-", "frame": 1, "gene": "lspA", "product": "signal peptidase II", "db_xrefs": [ "COG:COG0597", "COG:MU", "EC:3.4.23.36", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306", "KEGG:K03101", "SO:0001217", "UniRef:UniRef50_P75484", "UniRef:UniRef90_P75484" ], "nt": "ATGGCAAAAGCCCCAACTTTTTTTTCAAAATTGCTAAAGCAAATCCTTTTTGCTAACCGCAAACCCTTTTTGTACTATAAGCTTGCCTTAATCCTGTTTGTCAGTTTTGTCATTTTGTTTCAGGTATTTATGCTGCGTGCTGCCTTAAATGGTGAGAAGGGTATTAATGGTGCTAATGGTACAGATGTAGCTCGCAGTTCCTTTATTAGTATCTATGTTATCGGTAACAAGGGGGTTGGTTTTAGCTTATTGGCAGACCAACCAGGACTTGTTTACTTTCTCCAGGGTTTTCTATCTTTCATAGCACTGTTCTTTTTGGTTTTCTCTACTAGTTACAACTACATCTTTTGA", "aa": "MAKAPTFFSKLLKQILFANRKPFLYYKLALILFVSFVILFQVFMLRAALNGEKGINGANGTDVARSSFISIYVIGNKGVGFSLLADQPGLVYFLQGFLSFIALFFLVFSTSYNYIF", "aa_hexdigest": "9212a5f487d26899dfce32f9e9c61d0f", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [ "lspA" ], "truncated": "5-prime", "pseudogene": { "start": 348076, "stop": 348630, "observations": { "mutation": [ 348282 ], "stop": [ 348282 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75484", "query_cov": 1.0, "subject_cov": 0.6304347826086957, "identity": 0.991, "score": 214.0, "evalue": 2.67e-69, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MAKAPTFFSKLLKQILFANRKPFLYYKLALILFVGFVILFQVFMLRAALNGEKGINGANGTDVARSSFISIYVIGNKGVGFSLLADQPGLVYFLQGFLSFIALFFLVFSTSYNYIFWITTLAFGSLGNFFDRLTSGSGEVLDYFVFSGGNSVFNLADCCITFSFIGLFLSFLIQFFKEMKQTKS" }, "paralog": false, "identity": 0.9891304347826086, "score": 357.0, "evalue": 5.55e-128, "description": "Internal stop codon at 348282. Nonsense mutation around 348282.", "psc": { "uniref90_id": "UniRef90_P75484", "gene": "lspA", "product": "signal peptidase II", "ec_ids": [ "3.4.23.36" ], "uniref50_id": "UniRef50_P75484", "cog_id": "COG0597", "cog_category": "MU", "kegg_orthology_id": "K03101", "go_ids": [ "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75484", "EC:3.4.23.36", "UniRef:UniRef50_P75484", "COG:COG0597", "COG:MU", "KEGG:K03101", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ] }, "pscc": { "uniref50_id": "UniRef50_P75484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75484" ], "product": "Lipoprotein signal peptidase" } }, "id": "GIHJHMOALN_652", "locus": "GCA009939745_00617" }, { "type": "cds", "sequence": "contig_1", "start": 348669, "stop": 349289, "strand": "+", "frame": 3, "gene": "yajL", "product": "putative protein MPN_294", "db_xrefs": [ "COG:COG0693", "COG:V", "RefSeq:WP_010874651.1", "SO:0001217", "UniParc:UPI000013A574", "UniRef:UniRef100_P75483", "UniRef:UniRef50_P75483", "UniRef:UniRef90_P75483" ], "nt": "ATGGATTTGCTTACAACCGCAAACAAAACGCCGCGAATCGCCAAATCACGTCCTGTTCGAATTGCCATCATTATAACGCAGAAGACTAATGATCTGCACGCTACTGTACCCTGCTTTTTATGGCGCAAAGCAAGGTATGCTGTTGACCTGATTTCCGCTGAAGCCAAAGCTAGCATTATGTTAGAGATGGGGATTCATGTGCGCTGTGACAACACTTTGTCCAAAACTAACTTTAACCAGTATACCGCTGCTTTTATTCCCCATGGCAACACTACTAGGTTGGTGGAAATAGACAAACTAAGAAAGGACTTGGAGAAGTTTGTCTATAAACCCAAGGGACCAATGCGCTGGTTGTTTAGCAGTGGTAATGGTGCTTGTGTTTTGAAGGAGTTTGACTTGATTGCCCCTGACCAATTAGTCACCGTTCAAAACGAAAAGGAAATGGTTAAACTTTTGGGAAAAAATTTCATCAAGCAACCAGTACATGTGGACAAAAACATTATTAGTTGTGCCAATTCTTGTGGCTTGACTAAATTCTCCTTTAAGGTAATTGAAGAGCTTTCAGGAATAGAACTAGCGCGCAAAACGGCGAATCTTGTTGACCATATCTACAAAGGATAA", "aa": "MDLLTTANKTPRIAKSRPVRIAIIITQKTNDLHATVPCFLWRKARYAVDLISAEAKASIMLEMGIHVRCDNTLSKTNFNQYTAAFIPHGNTTRLVEIDKLRKDLEKFVYKPKGPMRWLFSSGNGACVLKEFDLIAPDQLVTVQNEKEMVKLLGKNFIKQPVHVDKNIISCANSCGLTKFSFKVIEELSGIELARKTANLVDHIYKG", "aa_hexdigest": "8eb1170c0b77bc3c543ae945ca8b91cc", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI000013A574", "ncbi_nrp_id": "WP_010874651.1", "uniref100_id": "UniRef100_P75483", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A574", "RefSeq:WP_010874651.1", "UniRef:UniRef100_P75483" ] }, "ips": { "uniref100_id": "UniRef100_P75483", "uniref90_id": "UniRef90_P75483" }, "psc": { "uniref90_id": "UniRef90_P75483", "gene": "YajL", "product": "Uncharacterized protein MPN_294", "uniref50_id": "UniRef50_P75483", "cog_id": "COG0693", "cog_category": "V" }, "pscc": { "uniref50_id": "UniRef50_P75483", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75483" ], "product": "Uncharacterized protein MPN_294" }, "genes": [ "yajL" ], "id": "GIHJHMOALN_653", "locus": "GCA009939745_00618" }, { "type": "cds", "sequence": "contig_1", "start": 349551, "stop": 349697, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCACTAAGCAAATTAAAGCTGGCAGTGTTAACGAAGTGTTAGCGCAAAAATTAGTCCAACTCGATGCGCTGCGCACTAACTATCTCATTGGCGCTTTGTACTCCACCAATAAAACACAAAAGAAGCTGTTTAAGCAATCGTGA", "aa": "MFTKQIKAGSVNEVLAQKLVQLDALRTNYLIGALYSTNKTQKKLFKQS", "aa_hexdigest": "9baa1dc809fb450e3e5ff1f7f9ef57b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5430.325400000001, "isoelectric_point": 10.09466915130615 }, "id": "GIHJHMOALN_654", "locus": "GCA009939745_00619" }, { "type": "cds", "sequence": "contig_1", "start": 349740, "stop": 350000, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTTAGTCGGTTTGCCGTTTGAAAAAGGGGAATATCATAAGCAACTGTATGCGCATTCCCGTCAAGAGATTTTGGATCTAGTGGAAGCGGTCAAAAAGCTGTATCTCAGACCAGAAAAGGATGACAAACTGGAGTTTGCTGATTCTTCCAAGGTAAGTGAGTCTAAGAGTATTAAGGTAACCAATGCAGTAACCTTACCAAGTGATGATTTAGATAAAGAGCTGTTTGAATTTAGCGGGGAAGGGGGTGATGAATAA", "aa": "MTLVGLPFEKGEYHKQLYAHSRQEILDLVEAVKKLYLRPEKDDKLEFADSSKVSESKSIKVTNAVTLPSDDLDKELFEFSGEGGDE", "aa_hexdigest": "5d5f7dade4ba0bf05da7074ab771a95f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9733.791899999997, "isoelectric_point": 4.793652915954591 }, "id": "GIHJHMOALN_655", "locus": "GCA009939745_00620" }, { "type": "cds", "sequence": "contig_1", "start": 350000, "stop": 350182, "strand": "+", "frame": 2, "gene": "rpsU", "product": "30S ribosomal protein S21", "db_xrefs": [ "COG:COG0828", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_010874653.1", "SO:0001217", "UniParc:UPI0000134E7D", "UniRef:UniRef100_P57079", "UniRef:UniRef50_Q8EUK4", "UniRef:UniRef90_P57079" ], "nt": "ATGCCAAAGATTGAAGTTAAAAACGATGATTTAGAGTTAGCTTTAAAAAAGTTTAAACGGGTTTCGCTAGAAATTCGCCGTTTAGCACAGCGTCACGAATACCACCTTCGAAAAGGAATGCGCTTGCGTGAAAAGCGAAAGATTGCGCAGAAGAAACGCCGTAAGTTTCGTAATATGGTCTAG", "aa": "MPKIEVKNDDLELALKKFKRVSLEIRRLAQRHEYHLRKGMRLREKRKIAQKKRRKFRNMV", "aa_hexdigest": "225e633bab1c8eb7c1d04d1005d5a625", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E7D", "ncbi_nrp_id": "WP_010874653.1", "uniref100_id": "UniRef100_P57079", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E7D", "RefSeq:WP_010874653.1", "UniRef:UniRef100_P57079" ] }, "ips": { "uniref100_id": "UniRef100_P57079", "uniref90_id": "UniRef90_P57079" }, "psc": { "uniref90_id": "UniRef90_P57079", "gene": "rpsU", "product": "30S ribosomal protein S21", "uniref50_id": "UniRef50_Q8EUK4", "cog_id": "COG0828", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EUK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EUK4" ], "product": "Small ribosomal subunit protein bS21" }, "genes": [ "rpsU" ], "id": "GIHJHMOALN_656", "locus": "GCA009939745_00621" }, { "type": "cds", "sequence": "contig_1", "start": 350207, "stop": 350656, "strand": "+", "frame": 2, "gene": null, "product": "putative MG211-like protein", "db_xrefs": [ "RefSeq:WP_014325447.1", "SO:0001217", "UniParc:UPI0001DD651E", "UniRef:UniRef100_A0AAX0SQI7", "UniRef:UniRef50_P47453", "UniRef:UniRef90_P75481" ], "nt": "ATGGCTAACGATAAAAAGGATAAGATTATTTTGAGTGGTGAGTTAACTAACCATCGCTTTAATTTTACCAAGGATGGGGAAAACGGCTATAGTGCTTATGAAGTAGACCGTTTTCTAGATCAGCTAGTTCACACTTTAACCCATTACGAAGCGCAGCGTAACCGTGAGGAGGAGATGAAAACGGCTTACGAAAAGCTCTTTCAAGACCGCGACGAAATTTTAAAGCGTTGCTCCAAACTAGAAGCAGAGCTCAATAACTTTTATGAAAACGGTTATTCCAACCGTGTGTTAATTAGTCGGGTTCAAGCCTTGGAAAACAAGATCGAAAGCCTCCCTAGTGGTCAAAATGACCGTCTAGAGCGCATCGAAAAGTTGCTAAAAAGGGTGATTAAGCACTGGACTGACGGGGAGGATTTATCCTATGGTGACTTCGATGATGATTTCTTTTAA", "aa": "MANDKKDKIILSGELTNHRFNFTKDGENGYSAYEVDRFLDQLVHTLTHYEAQRNREEEMKTAYEKLFQDRDEILKRCSKLEAELNNFYENGYSNRVLISRVQALENKIESLPSGQNDRLERIEKLLKRVIKHWTDGEDLSYGDFDDDFF", "aa_hexdigest": "a9346c4cb76ffb286b164bf8d335ef4a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD651E", "ncbi_nrp_id": "WP_014325447.1", "uniref100_id": "UniRef100_A0AAX0SQI7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD651E", "RefSeq:WP_014325447.1", "UniRef:UniRef100_A0AAX0SQI7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQI7", "uniref90_id": "UniRef90_P75481" }, "psc": { "uniref90_id": "UniRef90_P75481", "product": "Uncharacterized protein MG211 homolog", "uniref50_id": "UniRef50_P47453" }, "pscc": { "uniref50_id": "UniRef50_P47453", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47453" ], "product": "Uncharacterized protein MG211" }, "genes": [], "id": "GIHJHMOALN_657", "locus": "GCA009939745_00622" }, { "type": "cds", "sequence": "contig_1", "start": 350659, "stop": 351009, "strand": "+", "frame": 1, "gene": "acpS", "product": "holo-ACP synthase", "db_xrefs": [ "COG:COG0736", "COG:I", "EC:2.7.8.7", "GO:0000287", "GO:0005737", "GO:0006633", "GO:0008897", "SO:0001217", "UniRef:UniRef50_P75480", "UniRef:UniRef90_P75480" ], "nt": "ATGATTCTAGGCATAGGGATTGATTTAGTCGAAATCAAACGCTTTGAACAATTAGCTAGACAAACTGATAACTGTTTTGCGAAACGCTTGTTGACTTCAACGGAGTACGCGCACTATGCGAAGCTGCGTAAAGATAGTGAAAAGTCGAGTTTTTTAGCAGTCCACTGGTCATTAAAAGAAGCGATTTATAAGGCTGTTAACCACATCAAGCCATTGTTTAGCCAACTGGAAATTACAAAGAAAAACCAACGTTATAACTGCCAGATTGACCCTAAGATTGAACTTTTGCTCTCGGTTAGTTACAGCAGTAATAACATTACTGCCATCTGTTTGGCACAACAAACACCATGA", "aa": "MILGIGIDLVEIKRFEQLARQTDNCFAKRLLTSTEYAHYAKLRKDSEKSSFLAVHWSLKEAIYKAVNHIKPLFSQLEITKKNQRYNCQIDPKIELLLSVSYSSNNITAICLAQQTP", "aa_hexdigest": "6e0ae46a45c7e4b65b3016037358e773", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75480", "query_cov": 1.0, "subject_cov": 0.9747899159663865, "identity": 1.0, "score": 230.0, "evalue": 1.76e-76, "valid": true, "gene": "acpS", "product": "holo-ACP synthase", "ec_ids": [ "2.7.8.7" ], "uniref50_id": "UniRef50_P75480", "cog_id": "COG0736", "cog_category": "I", "go_ids": [ "GO:0005737", "GO:0008897", "GO:0000287", "GO:0006633" ] }, "pscc": { "uniref50_id": "UniRef50_P75480", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75480" ], "product": "Holo-[acyl-carrier-protein] synthase" }, "genes": [ "acpS" ], "id": "GIHJHMOALN_658", "locus": "GCA009939745_00623" }, { "type": "cds", "sequence": "contig_1", "start": 351006, "stop": 351383, "strand": "+", "frame": 3, "gene": "plsC", "product": "putative 1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "EC:2.3.1.51", "GO:0003841", "GO:0006654", "GO:0016020", "GO:0016024", "SO:0001217", "UniRef:UniRef50_Q49402", "UniRef:UniRef90_P75479" ], "nt": "ATGAAAAAATTAACACAAGCTTTTTTGCGTTTTTGTCTGAGATTCTTACAACTGCTCAGTTTAGTTTTGGTTTTACCCGTTTTTGTGTTAATGCTCATCTCTAGCCTAATTAGTGCTAAAAATTATGAAAGCATCCCGGAAAACTATCCCCCAGAAATCCGCTTTAAGAAGGTGTACCGCTTGGTATCACTTTTCCTCTACATTAAAGGAGTTAAAGTGGTTATTGTTAACCCTGAAAATGTGCCTAAAAAGGCTGTTTTAGTGGTAGCTAATCATAAGTCCAATTTAGATCCCCTCATCCTCATTAAGGCCTTTGGTAAAACTGAAGGGGTACCACCGTTAACCTTTATTGCCAAGATTGAACTGCAAGATACCTGA", "aa": "MKKLTQAFLRFCLRFLQLLSLVLVLPVFVLMLISSLISAKNYESIPENYPPEIRFKKVYRLVSLFLYIKGVKVVIVNPENVPKKAVLVVANHKSNLDPLILIKAFGKTEGVPPLTFIAKIELQDT", "aa_hexdigest": "49f75760ef28420f696218f266615553", "start_type": "ATG", "rbs_motif": null, "genes": [ "plsC" ], "truncated": "3-prime", "pseudogene": { "start": 351006, "stop": 351686, "observations": { "mutation": [ 351381 ], "stop": [ 351381 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75479", "query_cov": 1.0, "subject_cov": 0.4699248120300752, "identity": 1.0, "score": 228.0, "evalue": 1.28e-73, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MKKLTQAFLRFCLRFLQLLSLVLVLPVFVLMLISSLISAKNYESIPENYPPEIRFKKVYRLVSLFLYIKGVKVVIVNPENVPKKAVLVVANHKSNLDPLILIKAFGKTEGVPPLTFIAKIELQDTWLFKIMKLIDCVFIDRKNLRQMAASLEQQQQIIRQGTALCVFPEGTRVLSRQIGEFKSGALKVAYNAFVPIVPLTIVGSMGHMESKKRLQKAQVERDRGYKIQVIFNTPINPINFNQIDSQNVANNVWREISQTYAQYCQD" }, "paralog": false, "identity": 0.995575221238938, "score": 434.0, "evalue": 7.65e-157, "description": "Internal stop codon at 351381. Nonsense mutation around 351381.", "psc": { "uniref90_id": "UniRef90_P75479", "gene": "plsC", "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase", "ec_ids": [ "2.3.1.51" ], "uniref50_id": "UniRef50_Q49402", "cog_id": "COG0204", "cog_category": "I", "go_ids": [ "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75479", "EC:2.3.1.51", "UniRef:UniRef50_Q49402", "COG:COG0204", "COG:I", "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ] }, "pscc": { "uniref50_id": "UniRef50_Q49402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49402" ], "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "GIHJHMOALN_659", "locus": "GCA009939745_00624" }, { "type": "cds", "sequence": "contig_1", "start": 351396, "stop": 351806, "strand": "+", "frame": 3, "gene": "plsC", "product": "putative 1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "EC:2.3.1.51", "GO:0003841", "GO:0006654", "GO:0016020", "GO:0016024", "SO:0001217", "UniRef:UniRef50_Q49402", "UniRef:UniRef90_P75479" ], "nt": "ATGAAGCTGATTGACTGTGTCTTTATTGATCGAAAAAACCTGCGTCAAATGGCAGCATCCTTGGAACAACAGCAGCAAATTATCCGCCAGGGCACTGCTCTGTGTGTTTTTCCTGAGGGTACACGGGTACTCTCTAGGCAAATTGGTGAGTTTAAATCGGGCGCTTTAAAGGTCGCTTACAACGCCTTTGTGCCGATTGTGCCACTAACGATTGTGGGTAGCATGGGACACATGGAGTCCAAAAAGCGTTTACAAAAAGCCCAGGTAGAGCGCGATCGTGGTTATAAAATCCAGGTCATCTTTAACACACCTATTAACCCAATTAACTTTAACCAGATTGATTCGCAAAATGTGGCTAATAATGTCTGGCGTGAAATTAGTCAAACTTATGCGCAATATTGCCAAGACTAG", "aa": "MKLIDCVFIDRKNLRQMAASLEQQQQIIRQGTALCVFPEGTRVLSRQIGEFKSGALKVAYNAFVPIVPLTIVGSMGHMESKKRLQKAQVERDRGYKIQVIFNTPINPINFNQIDSQNVANNVWREISQTYAQYCQD", "aa_hexdigest": "5b0247043a500f3e2c5ff4c822c7bc1a", "start_type": "ATG", "rbs_motif": null, "genes": [ "plsC" ], "truncated": "5-prime", "pseudogene": { "start": 351096, "stop": 351806, "observations": { "mutation": [ 351381 ], "stop": [ 351381 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75479", "query_cov": 1.0, "subject_cov": 0.5112781954887218, "identity": 1.0, "score": 268.0, "evalue": 4.49e-89, "gene_start": 1, "gene_end": 136, "reference_start": 131, "reference_end": 266, "reference_sequence": "MKKLTQAFLRFCLRFLQLLSLVLVLPVFVLMLISSLISAKNYESIPENYPPEIRFKKVYRLVSLFLYIKGVKVVIVNPENVPKKAVLVVANHKSNLDPLILIKAFGKTEGVPPLTFIAKIELQDTWLFKIMKLIDCVFIDRKNLRQMAASLEQQQQIIRQGTALCVFPEGTRVLSRQIGEFKSGALKVAYNAFVPIVPLTIVGSMGHMESKKRLQKAQVERDRGYKIQVIFNTPINPINFNQIDSQNVANNVWREISQTYAQYCQD" }, "paralog": false, "identity": 0.9957627118644068, "score": 461.0, "evalue": 1.28e-167, "description": "Internal stop codon at 351381. Nonsense mutation around 351381.", "psc": { "uniref90_id": "UniRef90_P75479", "gene": "plsC", "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase", "ec_ids": [ "2.3.1.51" ], "uniref50_id": "UniRef50_Q49402", "cog_id": "COG0204", "cog_category": "I", "go_ids": [ "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75479", "EC:2.3.1.51", "UniRef:UniRef50_Q49402", "COG:COG0204", "COG:I", "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ] }, "pscc": { "uniref50_id": "UniRef50_Q49402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49402" ], "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "GIHJHMOALN_660", "locus": "GCA009939745_00625" }, { "type": "cds", "sequence": "contig_1", "start": 351930, "stop": 352259, "strand": "+", "frame": 3, "gene": null, "product": "dihydrofolate reductase", "db_xrefs": [ "EC:1.5.1.3", "KEGG:K00287", "SO:0001217", "UniRef:UniRef50_UPI000A8432F8", "UniRef:UniRef90_UPI000A8432F8" ], "nt": "ATGGGTTACAACACCTTTCAGGAGTTAGGTGACAAGCTAACTGATCACAACGTGGTGGTGTTGAGTAAAAAACACTTTGAAGAATTGCAAAACAACACCAACATTAAGGTGTTCAATTCAATTGAAAAGCTCTTGCAACACCACTTTAACCGTGATTTGTATGTGATTGGGGGCAAGCAAATCTTCCACCACTTTATTGAACTGGCTGATCGTTTAATTATTTCGGTTTTACCAGTGGACTTTAAGTGTAACCTTCGCTTAAAGTTGGGCTTGGACAGCTTTGAATTAATGCAAGAACAACAACACAGCCAATTTAAGGTGCAGTACTGA", "aa": "MGYNTFQELGDKLTDHNVVVLSKKHFEELQNNTNIKVFNSIEKLLQHHFNRDLYVIGGKQIFHHFIELADRLIISVLPVDFKCNLRLKLGLDSFELMQEQQHSQFKVQY", "aa_hexdigest": "583e60cf937ca482f3b2525aa60a4782", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 351807, "stop": 352344, "observations": { "insertion": [ 352311 ], "mutation": [ 351870, 352257 ], "stop": [ 351870, 352257 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A8432F8", "query_cov": 1.0, "subject_cov": 0.6089385474860335, "identity": 1.0, "score": 221.0, "evalue": 2.17e-72, "gene_start": 1, "gene_end": 109, "reference_start": 42, "reference_end": 150, "reference_sequence": "MITLIWCQDKHFGIGRDNTIPWKLTEANQHFYNTTKNQTVVMGYNTFQELGDKLTDHNVVVLSKKHFEELQNNTNIKVFNSIEKLLQHHFNRDLYVIGGKQIFHHFIELADRLIISVLPVDFKCNLRLKLGLDSFELMQEQQHSQFKVQYWHKKHPERLSFNVFLEDYXMGLYQTCWNF" }, "paralog": false, "identity": 0.9832402234636871, "score": 357.0, "evalue": 5.08e-128, "description": "Internal stop codon at 351870, 352257. Frameshift due to insertion around 352311. Nonsense mutation around 351870, 352257.", "psc": { "uniref90_id": "UniRef90_UPI000A8432F8", "product": "dihydrofolate reductase", "ec_ids": [ "1.5.1.3" ], "uniref50_id": "UniRef50_UPI000A8432F8", "kegg_orthology_id": "K00287", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A8432F8", "EC:1.5.1.3", "UniRef:UniRef50_UPI000A8432F8", "KEGG:K00287" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A8432F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A8432F8" ], "product": "dihydrofolate reductase" } }, "id": "GIHJHMOALN_661", "locus": "GCA009939745_00626" }, { "type": "cds", "sequence": "contig_1", "start": 352299, "stop": 352790, "strand": "+", "frame": 3, "gene": "scpA", "product": "segregation/condensation protein A", "db_xrefs": [ "KEGG:K05896", "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF", "UniRef:UniRef90_UPI000AF5CFDF" ], "nt": "TTGGAAGATTACAATGGGACTTTACCAAACTTGCTGGAACTTTTAATTGATAAGAAGTTCAACTTGCACCAGGTCGATATTGCCAAAATTACGACACAATACCTCCACTTAATTAACACTAACTTAAATAAACAAGCAATTGAACCAATTACCGACTACCTTGTCATTACTAGTCGAATTGTGGAGCAGAAGGCCAATAACTTACTGCAAATTAATGATATTGCCCTAGACTCTGACTTTTTAGACAACAAGCTGCGTGATAAACTAGTAGCACAGTTAGTGGAGTACAAACGTTACCGTGAATCACTCGATGACTTTGAGAAGCTAAGGATCAATCGCTTAGCTTACTTTTCCAAAGATAATGACTTTAACCGCTTTATCCAAACAGTTGACAAGAGTAATACAGAACCCGTGAAAATTGAGGACGAGCTACCCAATTATGTAAGTGTCTTAAAGTTGCATCACGCCATGAATAAGCTAATGCAGCGCTGA", "aa": "MEDYNGTLPNLLELLIDKKFNLHQVDIAKITTQYLHLINTNLNKQAIEPITDYLVITSRIVEQKANNLLQINDIALDSDFLDNKLRDKLVAQLVEYKRYRESLDDFEKLRINRLAYFSKDNDFNRFIQTVDKSNTEPVKIEDELPNYVSVLKLHHAMNKLMQR", "aa_hexdigest": "a1dd2b5742512480cabdfbcb33583015", "start_type": "TTG", "rbs_motif": null, "genes": [ "scpA" ], "truncated": "3-prime", "pseudogene": { "start": 352329, "stop": 353093, "observations": { "mutation": [ 352788 ], "stop": [ 352788 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "query_cov": 0.9386503067484663, "subject_cov": 0.4608433734939759, "identity": 0.993, "score": 299.0, "evalue": 8.16e-100, "gene_start": 11, "gene_end": 163, "reference_start": 1, "reference_end": 153, "reference_sequence": "MLELLIDKKFNLHQVDIAKITTQYLHLINTNLNKQAIEPITDYLVITSRIVEQKANNLLQINDIALDSDFLDNKLRDKLVAQLVEYKRYRESLDDFEKLRINRLAYFSKDNDFNRFIQTVDKSNTEPVKIEDELPNYVSVLKLHHAMNKLMQRWRAQFLANKNISIQELSIEQVQAEILATIKQFGYHSVSLKRVLLKVNHHISLMYFITAFVALLVLINNQIIDIEQTSFDDELYICLLDSSRIEQLQETPEAMVERAVKQRQEAQELARQVAREKAIANAQKREAYLKAKYGKDYLTREQFLKLSPEERAAHVAKMKQLKLVKNDNGRDN" }, "paralog": false, "identity": 0.9881889763779528, "score": 485.0, "evalue": 1.45e-175, "description": "Internal stop codon at 352788. Nonsense mutation around 352788.", "psc": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "gene": "scpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_UPI000AF5CFDF", "kegg_orthology_id": "K05896", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF5CFDF", "UniRef:UniRef50_UPI000AF5CFDF", "KEGG:K05896" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF5CFDF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "product": "segregation/condensation protein A" } }, "id": "GIHJHMOALN_662", "locus": "GCA009939745_00627" }, { "type": "cds", "sequence": "contig_1", "start": 352863, "stop": 353327, "strand": "+", "frame": 3, "gene": "scpA", "product": "segregation/condensation protein A", "db_xrefs": [ "KEGG:K05896", "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF", "UniRef:UniRef90_UPI000AF5CFDF" ], "nt": "TTGGCTACAATTAAGCAATTTGGCTATCATAGTGTTTCGTTAAAACGGGTACTGTTAAAGGTCAACCACCACATTTCCTTAATGTATTTCATTACCGCTTTTGTCGCTTTATTGGTGTTAATTAACAACCAGATTATCGACATTGAGCAGACTAGCTTTGATGATGAGCTTTACATCTGCTTGTTAGACAGTTCGCGGATTAAACAGCTCCAAGAAACGCCAGAAGCCATGGTGGAACGTGCTGTGAAACAACGCCAGGAAGCACAGGAATTAGCCCGTCAAGTAGCTAGAGAAAAAGCAATTGCCAACGCGCAGAAACGCGAAGCTTACCTAAAGGCAAAATATGGCAAGGATTACTTAACACGTGAACAGTTTTTAAAACTGTCTCCCGAAGAACGTGCTGCCCATGTGGCTAAAATGAAACAATTAAAACTAGTAAAGAACGACAATGGACGCGACAATTAA", "aa": "MATIKQFGYHSVSLKRVLLKVNHHISLMYFITAFVALLVLINNQIIDIEQTSFDDELYICLLDSSRIKQLQETPEAMVERAVKQRQEAQELARQVAREKAIANAQKREAYLKAKYGKDYLTREQFLKLSPEERAAHVAKMKQLKLVKNDNGRDN", "aa_hexdigest": "dd3b258a3c792f465deae3bef8eca132", "start_type": "TTG", "rbs_motif": "AACAA", "genes": [ "scpA" ], "truncated": "5-prime", "pseudogene": { "start": 352563, "stop": 353327, "observations": { "mutation": [ 352788 ], "stop": [ 352788 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "query_cov": 1.0, "subject_cov": 0.463855421686747, "identity": 0.987, "score": 274.0, "evalue": 4.15e-90, "gene_start": 1, "gene_end": 154, "reference_start": 179, "reference_end": 332, "reference_sequence": "MLELLIDKKFNLHQVDIAKITTQYLHLINTNLNKQAIEPITDYLVITSRIVEQKANNLLQINDIALDSDFLDNKLRDKLVAQLVEYKRYRESLDDFEKLRINRLAYFSKDNDFNRFIQTVDKSNTEPVKIEDELPNYVSVLKLHHAMNKLMQRWRAQFLANKNISIQELSIEQVQAEILATIKQFGYHSVSLKRVLLKVNHHISLMYFITAFVALLVLINNQIIDIEQTSFDDELYICLLDSSRIEQLQETPEAMVERAVKQRQEAQELARQVAREKAIANAQKREAYLKAKYGKDYLTREQFLKLSPEERAAHVAKMKQLKLVKNDNGRDN" }, "paralog": false, "identity": 0.9921259842519685, "score": 485.0, "evalue": 1.03e-175, "description": "Internal stop codon at 352788. Nonsense mutation around 352788.", "psc": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "gene": "scpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_UPI000AF5CFDF", "kegg_orthology_id": "K05896", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF5CFDF", "UniRef:UniRef50_UPI000AF5CFDF", "KEGG:K05896" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF5CFDF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "product": "segregation/condensation protein A" } }, "id": "GIHJHMOALN_663", "locus": "GCA009939745_00628" }, { "type": "cds", "sequence": "contig_1", "start": 353311, "stop": 353937, "strand": "+", "frame": 1, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304", "KEGG:K06024", "RefSeq:WP_010874658.1", "SO:0001217", "UniParc:UPI0000139488", "UniRef:UniRef100_P75477", "UniRef:UniRef50_P75477", "UniRef:UniRef90_P75477" ], "nt": "ATGGACGCGACAATTAAGGTAACTAAACCCGTTTTAAAACAAAAGGATAGCAGCGCTGCTAATTTAGTTGCTGCCATTTATGGGTTGTTGTTTGTAAGTGGCGAAAAGGGCTTAACGTTAGCGGAATTAAACCGCGTATTGCGCAAGGTGGGTTTGGAAAAGATTAAGGCCGCTTTAGTGCAGTTAGAGCGCAAGTTAAGTTTAGATGATGAAAGCGGGATTGAAATTAAAAAGTTTGGTCACAGCTTTCGCTTGGTCACCAAAATGGAGATCAAGGACTTTATCCACCGTTACCTACCCAACAAGATTAAAAATCCGCTCAATTCCAAAACAATGGAAGTGTTAGCAATTATTGCTTACAACCAACCCTGCACCCGTCCGCGCATTAATGAAATTAGGGGTGCAGATTCCTTTCAGATTGTCGATGACTTACTAGAAAAGGAGTTAATTGTTGAATTGGGCCGCAAAGACACACCTGGTCGTCCCTTTATTTATGAAGTGTCGCCTAAGTTCTATGATCTGTTTGGGATTAACAGCTTGGATGAACTGCCTAAAGTCGAGAATTTTGATCTCGACAAATTCCGCCAAGGTAGCTTTTTTGACTCTAACCGTTACGGGGATGATTAA", "aa": "MDATIKVTKPVLKQKDSSAANLVAAIYGLLFVSGEKGLTLAELNRVLRKVGLEKIKAALVQLERKLSLDDESGIEIKKFGHSFRLVTKMEIKDFIHRYLPNKIKNPLNSKTMEVLAIIAYNQPCTRPRINEIRGADSFQIVDDLLEKELIVELGRKDTPGRPFIYEVSPKFYDLFGINSLDELPKVENFDLDKFRQGSFFDSNRYGDD", "aa_hexdigest": "79f84eaf58ca309c2c01271745e51478", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139488", "ncbi_nrp_id": "WP_010874658.1", "uniref100_id": "UniRef100_P75477", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139488", "RefSeq:WP_010874658.1", "UniRef:UniRef100_P75477" ] }, "ips": { "uniref100_id": "UniRef100_P75477", "uniref90_id": "UniRef90_P75477" }, "psc": { "uniref90_id": "UniRef90_P75477", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_P75477", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304" ] }, "pscc": { "uniref50_id": "UniRef50_P75477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75477" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "GIHJHMOALN_664", "locus": "GCA009939745_00629" }, { "type": "cds", "sequence": "contig_1", "start": 353957, "stop": 354943, "strand": "+", "frame": 2, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005829", "GO:0005945", "GO:0006002", "GO:0016208", "GO:0030388", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0070095", "RefSeq:WP_010874659.1", "SO:0001217", "UniParc:UPI000012DB5C", "UniRef:UniRef100_P75476", "UniRef:UniRef50_P75476", "UniRef:UniRef90_P75476" ], "nt": "ATGAGTCCAAAAACAACCAAAAAAATTGCCATTTTAACCTCTGGTGGTGATGCTCCAGGGATGAATGCCACACTAGTTTACCTAACGCGTTATGCCACTAGTTCTGAAATTGAAGTTTTCTTTGTGAAAAACGGTTATTATGGGCTGTACCATGATGAGTTAGTACCCGCTCATCAATTAGATTTGTCAAACAGCTTGTTTAGTGCTGGCACCGTGATTGGTAGTAAGCGCTTTGTCGAATTTAAGGAGTTAAAGGTCCGCGAGCAAGCAGCACAGAATCTTAAAAAGCGCCAAATCGATTACCTAGTGGTTATTGGTGGTGATGGTAGTTACATGGGCGCTAAATTGCTAAGCGAGTTGGGCGTTAACTGTTACTGTTTGCCGGGTACGATTGACAACGACATAAATTCGAGTGAGTTTACAATTGGTTTTCTCACGGCTTTGGAGTCGATTAAGGTCAATGTCCAGGCCGTGTACCACACTACGAAATCACATGAACGGGTCGCCATTGTGGAGGTGATGGGTCGCCATTGTGGTGATTTAGCCATTTTTGGTGCCTTAGCCACCAATGCTGACTTTGTGGTGACTCCTAGCAATAAAATGGATTTAAAGCAGCTAGAAAGTGCTGTCAAAAAGATCTTGCAACACCAAAACCACTGTGTGGTGATTGTTAGTGAAAACATCTATGGCTTTGATGGTTATCCCTCACTTACAGCGATCAAACAGCACTTTGATGCTAACAACATGAAGTGTAACCTGGTGTCCTTGGGTCACACGCAACGCGGATTTGCCCCTACCAGCCTCGAGTTAGTCCAAATTAGTTTAATGGCGCAACACACGATTAACTTAATTGGACAAAACAAGGTTAACCAGGTCATTGGTAATAAGGCCAATGTGCCCGTTAACTATGACTTTGATCAAGCCTTTAACATGCCACCGGTTGATCGCAGCGCTTTAATTGCAGTCATTAATAAAAATATTATTTAA", "aa": "MSPKTTKKIAILTSGGDAPGMNATLVYLTRYATSSEIEVFFVKNGYYGLYHDELVPAHQLDLSNSLFSAGTVIGSKRFVEFKELKVREQAAQNLKKRQIDYLVVIGGDGSYMGAKLLSELGVNCYCLPGTIDNDINSSEFTIGFLTALESIKVNVQAVYHTTKSHERVAIVEVMGRHCGDLAIFGALATNADFVVTPSNKMDLKQLESAVKKILQHQNHCVVIVSENIYGFDGYPSLTAIKQHFDANNMKCNLVSLGHTQRGFAPTSLELVQISLMAQHTINLIGQNKVNQVIGNKANVPVNYDFDQAFNMPPVDRSALIAVINKNII", "aa_hexdigest": "626b61bc27bf7ea64beb6d495aa274b8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012DB5C", "ncbi_nrp_id": "WP_010874659.1", "uniref100_id": "UniRef100_P75476", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012DB5C", "RefSeq:WP_010874659.1", "UniRef:UniRef100_P75476" ] }, "ips": { "uniref100_id": "UniRef100_P75476", "uniref90_id": "UniRef90_P75476" }, "psc": { "uniref90_id": "UniRef90_P75476", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_P75476", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005945", "GO:0005829", "GO:0003872", "GO:0016208", "GO:0005524", "GO:0070095", "GO:0042802", "GO:0046872", "GO:0048029", "GO:0061621", "GO:0030388", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_P75476", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75476" ], "product": "Probable ATP-dependent 6-phosphofructokinase" }, "genes": [ "pfkA" ], "id": "GIHJHMOALN_665", "locus": "GCA009939745_00630" }, { "type": "cds", "sequence": "contig_1", "start": 355027, "stop": 355812, "strand": "+", "frame": 1, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0006096", "GO:0016020", "GO:0016301", "GO:0030955", "GO:0031639", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_P78031", "UniRef:UniRef90_P78031" ], "nt": "ATGTTAGATGACCCAGCTTACGCTGCGATGAAAGCAGAAGCTTACGCCAACATTGAAAATATTATTAAAAACGGCGTAACGGTAATCCGCTTAAACTTCTCCCATGGTAACCATGAAGAGCAGGCTGTAAGGATTAAGATTGTTCGTGATGTGGCTAAAAAGCTCAACCTCCCGGTGTCAATTATGTTAGACACCAACGGTCCGGAAATCCGCGTCTTTGAAACGGCCCCTGAAGGGTTAAAGATCCTCAAGGACAGTGAGGTGGTTATTAACACCACCACTAAGGAAGTGGCTAAAAATAACCAGTTTAGTGTGAGTGATGCTAGTGGCACTTACAACATGGTCAACGATGTTAAGGTGGGACAAAAAATCCTCGTTGATGATGGTAAGTTAAGCTTAGTCGTAAAGCGGATTGATACGAAGAACAACCAGGTTATTTGTGTTGCCCAAAATGACCACACTATCTTTACCAAAAAGCGCTTAAACCTCCCCAATGCCGACTACTCAATTCCCTTTTTAAGTGCTAAAGACCTACGCGACATTGACTTTGGTTTAACCCACCAAATTGACTACATTGCCGCTTCCTTTGTCAATACAACGGAAAACATCAAGCAACTGCGTGACTACCTCGCTTCTAAGAATGCCAAGCATGTCAAGCTTATTGCCAAAATTGAGTCCAACCACGCTTTAAACAACATTGATGGGATTATTAAAGCGAGTGATGGCATTATGGTAGCACGGGGTGATTTGGGCTTGGAGATCCCATATTACAAGGTGCCTTACTGA", "aa": "MLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSHGNHEEQAVRIKIVRDVAKKLNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTKEVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLVVKRIDTKNNQVICVAQNDHTIFTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKNAKHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPY", "aa_hexdigest": "6cb764dfa725510c574b570bf5492780", "start_type": "ATG", "rbs_motif": null, "genes": [ "pyk" ], "truncated": "both", "pseudogene": { "start": 354949, "stop": 356115, "observations": { "mutation": [ 355015, 355810 ], "stop": [ 355015, 355810 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78031", "query_cov": 1.0, "subject_cov": 0.5137795275590551, "identity": 1.0, "score": 509.0, "evalue": 1.28e-178, "gene_start": 1, "gene_end": 261, "reference_start": 27, "reference_end": 287, "reference_sequence": "MIHHLKRTKIIATCGPALTKKLWTLAMLDDPAYAAMKAEAYANIENIIKNGVTVIRLNFSHGNHEEQAVRIKIVRDVAKKLNLPVSIMLDTNGPEIRVFETAPEGLKILKDSEVVINTTTKEVAKNNQFSVSDASGTYNMVNDVKVGQKILVDDGKLSLVVKRIDTKNNQVICVAQNDHTIFTKKRLNLPNADYSIPFLSAKDLRDIDFGLTHQIDYIAASFVNTTENIKQLRDYLASKNAKHVKLIAKIESNHALNNIDGIIKASDGIMVARGDLGLEIPYYKVPYWQRYMIKACRFFNKRVITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDKQSETFFDYQYNLNYYMANSKARHSEFWKQVVLPLAQKTAPKRKLINSDFKYDFVVHATNNLNEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANVAIKHYQQHGEISQLKQCLGVFHNKIISL" }, "paralog": false, "identity": 0.9948453608247423, "score": 758.0, "evalue": 7.52e-279, "description": "Internal stop codon at 355015, 355810. Nonsense mutation around 355015, 355810.", "psc": { "uniref90_id": "UniRef90_P78031", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_P78031", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0005524", "GO:0006096", "GO:0016020", "GO:0016301", "GO:0030955", "GO:0031639" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78031", "EC:2.7.1.40", "UniRef:UniRef50_P78031", "COG:COG0469", "COG:G", "KEGG:K00873", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0006096", "GO:0016020", "GO:0016301", "GO:0030955", "GO:0031639" ] }, "pscc": { "uniref50_id": "UniRef50_P78031", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78031" ], "product": "Pyruvate kinase" } }, "id": "GIHJHMOALN_666", "locus": "GCA009939745_00631" }, { "type": "cds", "sequence": "contig_1", "start": 355822, "stop": 356115, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27" ], "nt": "ATGATAAAAGCGTGTCGTTTCTTTAACAAGCGTGTTATTACCGCAACTCAAATGTTGGACTCTTTGGAAAAAAACATCCAACCAACTCGCGCTGAAGTTACTGATGTGTACTTTGCCGTGGACCGCGGCAATGATGCCACCATGTTAAGTGGAGAAACCGCAAATGGGGCGTTTCCCCTTAATGCTGTGTATGTAATGAAGATGATTGATAAACAGTCCGAAACGTTCTTTGATTACCAGTACAACTTGAACTATTACATGGCCAACTCCAAGGCCCGTCACAGTGAGTTTTGA", "aa": "MIKACRFFNKRVITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETANGAFPLNAVYVMKMIDKQSETFFDYQYNLNYYMANSKARHSEF", "aa_hexdigest": "7c59f3e088c1d3bc519e2f601048efa3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 63, "aa_cov": 0.6494845360824743, "hmm_cov": 1.0, "evalue": 1.6146333627341641e-24, "score": 82.03192901611328, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 11198.605099999997, "isoelectric_point": 6.551739692687988 }, "id": "GIHJHMOALN_667", "locus": "GCA009939745_00632" }, { "type": "cds", "sequence": "contig_1", "start": 356122, "stop": 356475, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTGTTACCGTTAGCACAAAAAACAGCACCAAAACGCAAACTGATTAACAGTGACTTTAAATACGACTTTGTGGTGCACGCCACCAACAACCTTAATGAAATCTATGCCTTGTCCAACGCTCGCTTAGCGGCTGCGGTAATTATCTTGACCAATGACCCCCAAGTATACACCGGGCACGGCGTTGATTATGGCATCTTCCCTTACTTAATTGACCAAAAGCCGCAAAGCTTAAGTAAAGCTGAGTTTAAGAGCTTAGCTAATGTTGCCATTAAACACTACCAGCAGCATGGGGAAATTAGTCAATTAAAGCAGTGTTTGGGAGTATTTCACAATAAGATCATTAGCTTATAA", "aa": "MVLPLAQKTAPKRKLINSDFKYDFVVHATNNLNEIYALSNARLAAAVIILTNDPQVYTGHGVDYGIFPYLIDQKPQSLSKAEFKSLANVAIKHYQQHGEISQLKQCLGVFHNKIISL", "aa_hexdigest": "e13dcbdd189b0752fd87ad3cd15a034b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13110.00559999999, "isoelectric_point": 9.240269660949707 }, "id": "GIHJHMOALN_668", "locus": "GCA009939745_00633" }, { "type": "tRNA", "sequence": "contig_1", "start": 356515, "stop": 356591, "strand": "+", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 60.0, "nt": "GGTGCCTTAGCCAAGTGGATTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 356550, 356552 ], "id": "GIHJHMOALN_1", "locus": "GCA009939745_00634" }, { "type": "tRNA", "sequence": "contig_1", "start": 356643, "stop": 356719, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 83.1, "nt": "CGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 356660, 356662 ], "id": "GIHJHMOALN_2", "locus": "GCA009939745_00635" }, { "type": "tRNA", "sequence": "contig_1", "start": 356724, "stop": 356800, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 82.2, "nt": "GGCTGGATACCTCAGTTGGTTAGAGGACCCGGTTCATACCCGGGTTGTCGTGAGTTCGAATCTCACTCCAGCCACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 356758, 356760 ], "id": "GIHJHMOALN_3", "locus": "GCA009939745_00636" }, { "type": "tRNA", "sequence": "contig_1", "start": 356811, "stop": 356886, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 74.7, "nt": "GGATCTATAGCTCAATGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCGAGTCCTGTTAGATCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "GIHJHMOALN_4", "locus": "GCA009939745_00637" }, { "type": "tRNA", "sequence": "contig_1", "start": 356927, "stop": 357017, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 78.9, "nt": "GGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTGAAAAGCACGCGAGGGTTCGAATCCCTCAGTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 356946, 356948 ], "id": "GIHJHMOALN_5", "locus": "GCA009939745_00638" }, { "type": "tRNA", "sequence": "contig_1", "start": 357030, "stop": 357106, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 77.7, "nt": "CGCGGGATAGAGCAGTCGGTTAGCTCGTCAGGCTCATAATCTGAAGGTCGGGGGTTCGAATCCCTCTCCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "GIHJHMOALN_6", "locus": "GCA009939745_00639" }, { "type": "tRNA", "sequence": "contig_1", "start": 357108, "stop": 357181, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 55.6, "nt": "GGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 357140, 357142 ], "id": "GIHJHMOALN_7", "locus": "GCA009939745_00640" }, { "type": "tRNA", "sequence": "contig_1", "start": 357183, "stop": 357258, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 77.8, "nt": "GGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 357216, 357218 ], "id": "GIHJHMOALN_8", "locus": "GCA009939745_00641" }, { "type": "cds", "sequence": "contig_1", "start": 357455, "stop": 357730, "strand": "+", "frame": 2, "gene": null, "product": "arginine deiminase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132F68E0", "UniRef:UniRef90_UPI00132F68E0" ], "nt": "ATGAAGTACAACATCAACGTTCATTCCGAAATTGGTCAACTGCAAACCGTTTTAGTCCACACCCCTGGTAATGAAATTAGACGGATTTCACCAAGACGGTTGGATGATTTACTGTTTTCAGCAGTAATAGAACCAGATACTGCCATCCAGGAACACCAAACCTTTTGTCAGTTACTGCAAGAACAAAACATTGAAGTGGTGCAACTGACTGATTTAACAGCCACCACCTTTGACAAGGCAAATGCTACAGCGCAAAACCAGTTTATTGAAACTTGA", "aa": "MKYNINVHSEIGQLQTVLVHTPGNEIRRISPRRLDDLLFSAVIEPDTAIQEHQTFCQLLQEQNIEVVQLTDLTATTFDKANATAQNQFIET", "aa_hexdigest": "e88084dfe637df752a322788b98e60e0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 357455, "stop": 358009, "observations": { "mutation": [ 357728 ], "stop": [ 357728 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00132F68E0", "query_cov": 1.0, "subject_cov": 0.4945652173913043, "identity": 1.0, "score": 179.0, "evalue": 9.54e-56, "gene_start": 1, "gene_end": 91, "reference_start": 1, "reference_end": 91, "reference_sequence": "MKYNINVHSEIGQLQTVLVHTPGNEIRRISPRRLDDLLFSAVIEPDTAIQEHQTFCQLLQEQNIEVVQLTDLTATTFDKANATAQNQFIETWLDQAEPKLTPEHRKVAKQYLLEQKAKSTLSMVRSMMGGIDKRKVAAANTINGDFLVDPMPNLYFTRDPFASIGHGISINRMKYLTRRRQTLF" }, "paralog": false, "identity": 0.9891304347826086, "score": 360.0, "evalue": 1.31e-129, "description": "Internal stop codon at 357728. Nonsense mutation around 357728.", "psc": { "uniref90_id": "UniRef90_UPI00132F68E0", "product": "arginine deiminase family protein", "uniref50_id": "UniRef50_UPI00132F68E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00132F68E0", "UniRef:UniRef50_UPI00132F68E0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00132F68E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00132F68E0" ], "product": "arginine deiminase family protein" } }, "id": "GIHJHMOALN_669", "locus": "GCA009939745_00642" }, { "type": "cds", "sequence": "contig_1", "start": 357821, "stop": 358135, "strand": "+", "frame": 2, "gene": null, "product": "Arginine deiminase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCR3", "UniRef:UniRef90_A0AAN5BCR3" ], "nt": "ATGGTACGCTCGATGATGGGTGGGATTGATAAGCGTAAGGTAGCTGCTGCTAACACCATTAACGGTGATTTTTTAGTAGATCCGATGCCTAACCTTTACTTTACCCGCGATCCGTTTGCCTCGATTGGCCATGGTATTAGTATTAACCGGATGAAGTATTTAACGCGGCGGCGTGAAACCTTATTTGCTAGTTTTATTTTGCCAACCACCCCGATTATTGCCGCACGCAAATTTACTTTAAACCAATTGACATGGGCACAATTGAGGGTGGTGATATTTTTGTATATGACCAACAAACAGTGGTGGTGGGGTTGA", "aa": "MVRSMMGGIDKRKVAAANTINGDFLVDPMPNLYFTRDPFASIGHGISINRMKYLTRRRETLFASFILPTTPIIAARKFTLNQLTWAQLRVVIFLYMTNKQWWWG", "aa_hexdigest": "d91aeb3693cb177712dd3533e303bb58", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 357521, "stop": 358171, "observations": { "mutation": [ 357728, 358133 ], "stop": [ 357728, 358133 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN5BCR3", "query_cov": 1.0, "subject_cov": 0.4369747899159664, "identity": 1.0, "score": 215.0, "evalue": 3.24e-69, "gene_start": 1, "gene_end": 104, "reference_start": 123, "reference_end": 226, "reference_sequence": "MKYNINVHSEIGQLQTVLVHTPGNEIRRISPRRLDDLLFSAVIEPDTAIQEHQTFCQLLQEQNIEVVQLTDLTATTFDKANATAQNQFIETWLDQAEPKLTPEHRKVAKQYLLEQKAKSTLSMVRSMMGGIDKRKVAAANTINGDFLVDPMPNLYFTRDPFASIGHGISINRMKYLTRRRETLFASFILPTTPIIAARKFTLNQLTWAQLRVVIFLYMTNKQWWWGWVNGQPKLPLMF" }, "paralog": false, "identity": 0.9907407407407407, "score": 427.0, "evalue": 6.82e-155, "description": "Internal stop codon at 357728, 358133. Nonsense mutation around 357728, 358133.", "psc": { "uniref90_id": "UniRef90_A0AAN5BCR3", "product": "Arginine deiminase domain-containing protein", "uniref50_id": "UniRef50_A0AAN5BCR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN5BCR3", "UniRef:UniRef50_A0AAN5BCR3" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAN5BCR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN5BCR3" ], "product": "Arginine deiminase domain-containing protein" } }, "id": "GIHJHMOALN_670", "locus": "GCA009939745_00643" }, { "type": "cds", "sequence": "contig_1", "start": 358120, "stop": 358365, "strand": "+", "frame": 1, "gene": null, "product": "Arginine deiminase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742", "UniRef:UniRef90_A0AB38W742" ], "nt": "GTGGTGGTGGGGTTGAGTGAACGGACAACCGAAGCTGCCATTAATGTTTTAGCGAAAAAGATTCAACAAGACAGTAGCACTTCCTTTAAGCGGATCTTTGTCATTAACGTTCCACAACTACCGAACTTAATGCATTTAGACACGTGGCTGACAATGTTAGACCGCAACAAGTTCTTGTACTCCCCGAACATGTTGGCGGTTTTAAAGGCGTGGCGGATTGACTTGACTGATCCGGCTTTAAAGTGA", "aa": "MVVGLSERTTEAAINVLAKKIQQDSSTSFKRIFVINVPQLPNLMHLDTWLTMLDRNKFLYSPNMLAVLKAWRIDLTDPALK", "aa_hexdigest": "05457bc82bc16dba783c736c0e48e5fb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 358072, "stop": 358668, "observations": { "mutation": [ 358363 ], "stop": [ 358363 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W742", "query_cov": 1.0, "subject_cov": 0.4090909090909091, "identity": 0.988, "score": 160.0, "evalue": 1.91e-48, "gene_start": 1, "gene_end": 81, "reference_start": 17, "reference_end": 97, "reference_sequence": "MGTIEGGDIFVYDQQTVVVGLSERTTEAAINVLAKKIQQDSSTSFKRIFVINVPQLPNLMHLDTWLTMLDRNKFLYSPNMLAVLKAWRIDLTDPALKWNEIAGDLSTILHTIIGQKPMLIPIAGADANQTEIDIETHFDGTNYLTIAPSVVVGYARNKLTHQALEAAGVKVIAFKGNQLSLGMGSARCMSMPLVRKPL" }, "paralog": false, "identity": 0.9949494949494949, "score": 387.0, "evalue": 2.27e-140, "description": "Internal stop codon at 358363. Nonsense mutation around 358363.", "psc": { "uniref90_id": "UniRef90_A0AB38W742", "product": "Arginine deiminase", "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W742", "UniRef:UniRef50_A0AB38W742" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742" ], "product": "Arginine deiminase" } }, "id": "GIHJHMOALN_671", "locus": "GCA009939745_00644" }, { "type": "cds", "sequence": "contig_1", "start": 358423, "stop": 358668, "strand": "+", "frame": 1, "gene": null, "product": "Arginine deiminase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742", "UniRef:UniRef90_A0AB38W742" ], "nt": "ATGCTCATTCCCATTGCCGGAGCGGATGCTAACCAAACCGAAATTGACATTGAAACCCACTTTGATGGCACCAACTACCTCACCATCGCTCCATCGGTAGTAGTGGGGTATGCGCGCAATAAATTAACACACCAGGCCTTGGAAGCGGCTGGGGTTAAAGTAATTGCCTTTAAGGGGAACCAGCTGTCTTTGGGAATGGGTTCTGCACGTTGTATGTCGATGCCATTAGTACGCAAACCACTTTAA", "aa": "MLIPIAGADANQTEIDIETHFDGTNYLTIAPSVVVGYARNKLTHQALEAAGVKVIAFKGNQLSLGMGSARCMSMPLVRKPL", "aa_hexdigest": "fdbd022901da6bce238cd7922f0467da", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 358123, "stop": 358668, "observations": { "mutation": [ 358363 ], "stop": [ 358363 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W742", "query_cov": 1.0, "subject_cov": 0.4090909090909091, "identity": 1.0, "score": 161.0, "evalue": 9.48e-49, "gene_start": 1, "gene_end": 81, "reference_start": 118, "reference_end": 198, "reference_sequence": "MGTIEGGDIFVYDQQTVVVGLSERTTEAAINVLAKKIQQDSSTSFKRIFVINVPQLPNLMHLDTWLTMLDRNKFLYSPNMLAVLKAWRIDLTDPALKWNEIAGDLSTILHTIIGQKPMLIPIAGADANQTEIDIETHFDGTNYLTIAPSVVVGYARNKLTHQALEAAGVKVIAFKGNQLSLGMGSARCMSMPLVRKPL" }, "paralog": false, "identity": 0.994475138121547, "score": 353.0, "evalue": 8.08e-127, "description": "Internal stop codon at 358363. Nonsense mutation around 358363.", "psc": { "uniref90_id": "UniRef90_A0AB38W742", "product": "Arginine deiminase", "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W742", "UniRef:UniRef50_A0AB38W742" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W742" ], "product": "Arginine deiminase" } }, "id": "GIHJHMOALN_672", "locus": "GCA009939745_00645" }, { "type": "cds", "sequence": "contig_1", "start": 358696, "stop": 358893, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATTAACTTAAAAGGTCGTAGCCTTGACTCTGCTTTAAACTTTACTACTGCTCAAATTAACTATTTAATTGACCTCGCCATTGATTTAAAACGCAGTAAATACCAAGTTGCACATCCAGAACCGTCCCTTAGTGGGGAAGAACATTGTTTTACTGTTCCAAAAAGATTCTACCCGTACCCGTTGTGCCTTTGA", "aa": "MPINLKGRSLDSALNFTTAQINYLIDLAIDLKRSKYQVAHPEPSLSGEEHCFTVPKRFYPYPLCL", "aa_hexdigest": "dc06425fa9cd9654cf3f7b8eb3c9d9a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7425.520999999999, "isoelectric_point": 7.799336814880372 }, "id": "GIHJHMOALN_673", "locus": "GCA009939745_00646" }, { "type": "cds", "sequence": "contig_1", "start": 358832, "stop": 359080, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02729.26" ], "nt": "GTGGGGAAGAACATTGTTTTACTGTTCCAAAAAGATTCTACCCGTACCCGTTGTGCCTTTGAAGTGGCTGCCTTTGACTTGGGTATGGGTTGCACTTACATCGGCCCTTCTGGTTCCAATTTTGGTAAGAAGGAATCGATTGAAGACACTGCTAAAGTGTTGGGTTCAATGTATGACGGAATTGAATTCCGTGGCTTTAAGCAAAGCGATGTTGATGCTTTGGTAAAGTACTCTGGTGTTCCTGTTTGA", "aa": "MGKNIVLLFQKDSTRTRCAFEVAAFDLGMGCTYIGPSGSNFGKKESIEDTAKVLGSMYDGIEFRGFKQSDVDALVKYSGVPV", "aa_hexdigest": "7fd32847496358fd8ec335c9af44c23c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02729.26", "name": "OTCace_N", "length": 87, "aa_cov": 0.9878048780487805, "hmm_cov": 1.0, "evalue": 1.1723018636947005e-24, "score": 82.61307525634766, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 8894.083699999996, "isoelectric_point": 6.150058555603027 }, "id": "GIHJHMOALN_674", "locus": "GCA009939745_00647" }, { "type": "cds", "sequence": "contig_1", "start": 359087, "stop": 359419, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00185.30" ], "nt": "TTGACTGATGCGGAACACCCTACCCAAATGCTAGCGGATTACATGACGATTAAGGAGTTGAAGGGTGATTTGAAGGGGCGCAAAATTGTCTTTGCTGGTGATATTAAAAATAATGTGGCACGCTCGTTGATGATTGGGGCAGCTTTTGTTGGTATGGACATAGTGCTAGTAGGTCCTAAAGCACAGCATGAATTAGTTCAAAATGGTGCTGGGTATAAAGAGGTCTTTGAGCAGTGTCAAGCATTGTTTCAACTCAATGGTGGTAGTGTTAGCTTTAGCACTGATAAGCTGCAAGCGGCTAAGAATGCTGATGTAATTTACACCGATGTGTGA", "aa": "MTDAEHPTQMLADYMTIKELKGDLKGRKIVFAGDIKNNVARSLMIGAAFVGMDIVLVGPKAQHELVQNGAGYKEVFEQCQALFQLNGGSVSFSTDKLQAAKNADVIYTDV", "aa_hexdigest": "334c7426cbdec7e6abf218bd0e67c9d4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00185.30", "name": "OTCace", "length": 86, "aa_cov": 0.7818181818181819, "hmm_cov": 0.9186046511627907, "evalue": 2.550390267288773e-19, "score": 65.3192138671875, "start": 25, "stop": 110 } ], "seq_stats": { "molecular_weight": 11936.594999999994, "isoelectric_point": 5.569902992248534 }, "id": "GIHJHMOALN_675", "locus": "GCA009939745_00648" }, { "type": "cds", "sequence": "contig_1", "start": 359426, "stop": 359737, "strand": "+", "frame": 2, "gene": null, "product": "Ornithine carbamoyltransferase chain F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "nt": "TTGGGTGAAGACTTTAGCTTATTTGAAGAACGGATTCAGGAGCTGGGTCAATTTCAAGTTGACATGGCTATGATCAAAGCAGCTAAGAGTGATGTGATCTTCTTGCACTGTCTCCCCGCTTTCCATGATGATCACACTAGCTTTTCACTGGAAATTAAACAAAAGTTAGGTAAAAAATATCCGGTGGTGAAAACGGGTGCGATGGAAGTGACTGATACGGTGTTTCAGTCCAAGCATAACATGGCCTTTATCCAAGCAGAAAACCGCTTGCACACGATTAAGGCCGTTATTTTAGCTACTTTAGGGTATTAA", "aa": "MGEDFSLFEERIQELGQFQVDMAMIKAAKSDVIFLHCLPAFHDDHTSFSLEIKQKLGKKYPVVKTGAMEVTDTVFQSKHNMAFIQAENRLHTIKAVILATLGY", "aa_hexdigest": "f2e0a1078b4b5f84a9b7db0a05cf2ea8", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4U9HLN2", "query_cov": 0.8349514563106796, "subject_cov": 0.9885057471264368, "identity": 0.5, "score": 79.0, "evalue": 2.91e-17, "valid": false, "product": "Ornithine carbamoyltransferase chain F", "uniref50_id": "UniRef50_A0A4U9HLN2" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9HLN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "product": "Ornithine carbamoyltransferase chain F" }, "genes": [], "id": "GIHJHMOALN_676", "locus": "GCA009939745_00649" }, { "type": "cds", "sequence": "contig_1", "start": 359740, "stop": 360669, "strand": "+", "frame": 1, "gene": "arcC", "product": "Carbamate kinase-like protein", "db_xrefs": [ "COG:COG0549", "COG:E", "GO:0005829", "GO:0008804", "GO:0019546", "RefSeq:WP_010874663.1", "SO:0001217", "UniParc:UPI0000125DAF", "UniRef:UniRef100_P78030", "UniRef:UniRef50_P78030", "UniRef:UniRef90_P78030" ], "nt": "ATGCAAAAAATTGTCATAGCATTGGGTGGTAATGCATTAGGTAACAATCCCACCGAACAAAAGGACTTGGTACAACTACCAGCACGACAGGCGGTTGCTTTGTTAAAGCAGGGTTATCAGATATTATTGGGTCATGGTAACGGTCCACAGGTTGGCATGATCTACAACGCCTTTAGTGCGGCCAAACAAGTCAACCCTAATACACCCACTGTACCATTTGCTGAAAGTGGGGCAATGTCTCAGGGTTACATTGGACTGCATTTGTTAACAGCATTGTACAATGAGCTGCTACACCAAAAACTACCCCATCAAGCCGTGTACTTTTTAACACAAACCTTAGTAGAAGCGAGCGATCCTGCTTTTCAAAACCCAAATAAACCAGTTGGTCCGTTTTACAACACAGAGGAAACAGCACGCTCAGCTAATCCAAACAGCACCGTGGTGGAAGATGCAGGGCGGGGTTGGCGTAAAGTAGTAGCGTCCCCCAAACCAGTTGATGTTTTGGGAATTGATGCCATTAAAAGTAGCTTTAACCAAGGCAACTTGGTGATTGTTGGTGGCGGTGGCGGTGTACCCACAATTAAGACCAAAAGTGGTTATGCAACGGTTGATGGGGTGATTGATAAGGATCTAGCATTGAGTGAAATTGCCATTAAGGTAGAGGCTGACCTGTTTGTTATTCTCACTGCCGTGGACTTTGTCTACATTAACTATGGTCAACCAAACGAGCAAAAGCTAACCTGCATTAACACCAAGGAAGCTAAGACCTTAATGGCCGCAAACCAGTTTGCTAAAGGTAGTATGCTCCCCAAGGTAGAAGCGTGTTTAAACTTTGTCCAGTCGGGTACCAATAAGACGGCCATTATTGCCCAATTAGACCAATTAGCTGCGGCCATTGCGGGCAAGATTGGTACTAAAATTGTCAAGTAG", "aa": "MQKIVIALGGNALGNNPTEQKDLVQLPARQAVALLKQGYQILLGHGNGPQVGMIYNAFSAAKQVNPNTPTVPFAESGAMSQGYIGLHLLTALYNELLHQKLPHQAVYFLTQTLVEASDPAFQNPNKPVGPFYNTEETARSANPNSTVVEDAGRGWRKVVASPKPVDVLGIDAIKSSFNQGNLVIVGGGGGVPTIKTKSGYATVDGVIDKDLALSEIAIKVEADLFVILTAVDFVYINYGQPNEQKLTCINTKEAKTLMAANQFAKGSMLPKVEACLNFVQSGTNKTAIIAQLDQLAAAIAGKIGTKIVK", "aa_hexdigest": "993d22e63285c239bdd26b61078511c6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125DAF", "ncbi_nrp_id": "WP_010874663.1", "uniref100_id": "UniRef100_P78030", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125DAF", "RefSeq:WP_010874663.1", "UniRef:UniRef100_P78030" ] }, "ips": { "uniref100_id": "UniRef100_P78030", "uniref90_id": "UniRef90_P78030" }, "psc": { "uniref90_id": "UniRef90_P78030", "gene": "ArcC", "product": "Carbamate kinase-like protein", "uniref50_id": "UniRef50_P78030", "cog_id": "COG0549", "cog_category": "E", "go_ids": [ "GO:0005829", "GO:0008804", "GO:0019546" ] }, "pscc": { "uniref50_id": "UniRef50_P78030", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78030" ], "product": "Carbamate kinase-like protein" }, "genes": [ "arcC" ], "id": "GIHJHMOALN_677", "locus": "GCA009939745_00650" }, { "type": "cds", "sequence": "contig_1", "start": 360672, "stop": 361475, "strand": "+", "frame": 3, "gene": null, "product": "amino acid permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244EE166" ], "nt": "ATGAAACAACAAAAACCCAAAATTAGCTTTATAGCAGCGATGCTAATTGTTATCGGCTCGTCCATAGGTGCTGGTATCTTTTTTAAATCCAGCACTGTTTTGGAAAATTCCCAGGCCTCTTTAGTGCTAGCGATTTTTAACTGGTTGGTAGCTTCAGTGGCAGTGATTGCTATGGCCTTGGCATTAATTGAAATTGCCAGTGTTCGCAACGATAATCTCTCGATTATTAGCTGGGTCAAAGTGTTTAACCGGCGCTGGTTGTACCATGGTTGTAAGAATTTTATGACCTATTTGTACCTACCGTTGACCTTCTTCTTTATGCCGCTGTACTTTATCTGTTCCATTCAAGATGGGTTTCGCGGTTTGTTGGGACTGGAAACCGGAGCGCATTTCAATACTAGTGTGGACTGGTTAATCTGGTTAGCTTTAGCACTTATTATTACCACTTACTTCTTGACAATTCCACCACTGTACGCTAAGGTAGGGAACATCCAAAACATGGTGGTAAGCGCCGTGAAGTTTATTCCTTTAGTGTTTGTGCCTATCATCGGTTTTATTGTAGCGGGTACAGGTAACGGTGAATTAAAAAACGTTAAGGCATTAGTGCAACCCCCACAAATTAATGGTGCGACTGCTTCCTTTACCCAATTAGTACAAGCGGGGTATGGAATTACTCGTTTTACGGGAATTGGTGCTGGGATGGGTTCCTTTATTTCGATTGCCGCCATTTTCTTTGCGTACGATGGTTTTTACGTAACAGCTGGACTGCAGTCAGAGATGCGTGAACCGAAAAAAACACCGTGA", "aa": "MKQQKPKISFIAAMLIVIGSSIGAGIFFKSSTVLENSQASLVLAIFNWLVASVAVIAMALALIEIASVRNDNLSIISWVKVFNRRWLYHGCKNFMTYLYLPLTFFFMPLYFICSIQDGFRGLLGLETGAHFNTSVDWLIWLALALIITTYFLTIPPLYAKVGNIQNMVVSAVKFIPLVFVPIIGFIVAGTGNGELKNVKALVQPPQINGATASFTQLVQAGYGITRFTGIGAGMGSFISIAAIFFAYDGFYVTAGLQSEMREPKKTP", "aa_hexdigest": "ccea19e1dcd65445d1d7b1eb7f7c3e4b", "start_type": "ATG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_UPI00244EE166", "query_cov": 0.9775280898876404, "subject_cov": 0.8642384105960265, "identity": 0.552, "score": 275.0, "evalue": 3.12e-89, "valid": false, "product": "amino acid permease", "uniref50_id": "UniRef50_UPI00244EE166" }, "pscc": { "uniref50_id": "UniRef50_UPI00244EE166", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244EE166" ], "product": "amino acid permease" }, "genes": [], "id": "GIHJHMOALN_678", "locus": "GCA009939745_00651" }, { "type": "cds", "sequence": "contig_1", "start": 361479, "stop": 361682, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTAGGTTTACTAATTACTACGCTGTTTTACCTTATCTTAGCAGTGGCACTTTCCATTAACGGTGGTTTGTTTAGTGGTATGGAAGAGTCCATGGGAAAGCTGTTTAACAATAAGCGTGCCGGGCAAATTGTCTTTGGTGTTGTTAACCTAATGATTGGCATAGGTGTCTTGGGTATTATTAACGGTTTTGCACTGTGA", "aa": "MFLGLLITTLFYLILAVALSINGGLFSGMEESMGKLFNNKRAGQIVFGVVNLMIGIGVLGIINGFAL", "aa_hexdigest": "33b2f0ba50e7efbad43f86d9b5ef9918", "start_type": "TTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7092.5383999999995, "isoelectric_point": 8.25416126251221 }, "id": "GIHJHMOALN_679", "locus": "GCA009939745_00652" }, { "type": "cds", "sequence": "contig_1", "start": 361740, "stop": 361949, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGGGCAGATTGAACCCCAACAAGCCAGTGGTGGGGGTAATTTACTGTTTGGTACTTTCTTTGACTGTTCAAGTGCTGTTTACGGTAATAGGTGCTTTGGCTTACCTCCCGACAGTAGCGGATTACAAAAATTACGTTAACACTGAAATTGACAAGCTCAATTCGATGCAATGGTTGTATTCGTTTTCCGATTTAATGGCTACTTGA", "aa": "MQGRLNPNKPVVGVIYCLVLSLTVQVLFTVIGALAYLPTVADYKNYVNTEIDKLNSMQWLYSFSDLMAT", "aa_hexdigest": "e0149582a18217d9572dc2a154a50fd6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7745.0167, "isoelectric_point": 5.916052436828613 }, "id": "GIHJHMOALN_680", "locus": "GCA009939745_00653" }, { "type": "cds", "sequence": "contig_1", "start": 362076, "stop": 362369, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTAACTGCATTAGTGTTTTTGTAACTATCATTGAGCCCTTCATTAACCTCTTTTTACTGTTTGGTTATGATGAAACAGTGGCACACACAGTGCTCGGTAATGACTTCATTGAATTGAATGAATTAGTGATAGGACGGGTAATGCTGATAGTAGTGTTAGTTTTCTTTGCCATAATTAGCTTTCTCCCTGTTTATGTGGAGGATCAATACCACAAACGTAAGTTTGGTAGTTTAGCTAACTATCAGCAATATGTACAACAACATTTAGCACACTCCACCATTAACGGTTAA", "aa": "MVNCISVFVTIIEPFINLFLLFGYDETVAHTVLGNDFIELNELVIGRVMLIVVLVFFAIISFLPVYVEDQYHKRKFGSLANYQQYVQQHLAHSTING", "aa_hexdigest": "f9b3ac9992a53fa9f0deb599d6c69132", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11138.88329999999, "isoelectric_point": 5.32839412689209 }, "id": "GIHJHMOALN_681", "locus": "GCA009939745_00654" }, { "type": "cds", "sequence": "contig_1", "start": 362425, "stop": 363063, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCGGTACTAGTAACATAGTAAGGATCGGTGTAAGCGTTTGGGTCCTGATAAGCATTGGGATCTACATAGGCGTTAAGATCGGTGTAAGCGTTTGGGTCCTGATAAGCATTGGGATCGGCATAGTACGCTTGGTTGGGATCTTGAGCATAGTCTTGTGGATATGCTTGACCATCCTGACCATAGCCGTAATAAGCATTGGGATCGGTGTAGTTATTAGGATCTACATAAGCGTTTGGGTCCTGATAAGCATTGGGATCTACATAGGCGTTAGGATCGGTGTAAGCGTTTGGGTCCTGATAAGCATTGGGATCGGCATAATACGCTTGGTTGGGATCTTGAGCATAGTCTTGTGGATATGCTTGACCATCCTGACCATAGCCGTAATAAGCCGCTGCATCAGGATAGGTATTCGGATCGGGGTAATACGCTTGGTTGGGATCAACTGCTTGGTCGGGGTCGTAATAAGTACTACCATCACCATAGTACTGCTGCTGATTGGGATCGTATGCAGTAGCTTGTTGGTCAGACTGATTTCAACCTGGTTTATTTATATCCATTTACTGTCTTTAAAAGGTGCCAACAGCAAAGCTGACACTATATCATTAATTTTAATTTATCTAAAAAAATGCAAATGA", "aa": "MIGTSNIVRIGVSVWVLISIGIYIGVKIGVSVWVLISIGIGIVRLVGILSIVLWICLTILTIAVISIGIGVVIRIYISVWVLISIGIYIGVRIGVSVWVLISIGIGIIRLVGILSIVLWICLTILTIAVISRCIRIGIRIGVIRLVGINCLVGVVISTTITIVLLLIGIVCSSLLVRLISTWFIYIHLLSLKGANSKADTISLILIYLKKCK", "aa_hexdigest": "afb2e17e9b4c9d6b3464d208918dc958", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22830.327700000045, "isoelectric_point": 10.419009590148924 }, "id": "GIHJHMOALN_682", "locus": "GCA009939745_00655" }, { "type": "cds", "sequence": "contig_1", "start": 363307, "stop": 363504, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCAAAGCGATTGATACTGTCAAGGAAAACAGCGCTAACTTGCAAATTATGAAGCAGAACTTTGAACGCCAACTCAAAGAACAGCAAACCCAACGGCTTACAAGTAATACTGACGCGGAAAAGATTGGTGCTAAGATCAACCAATTAGAGGAACGCATGCAACGTTTATCGCGCACTATGGAAAGTGTGGAGTGA", "aa": "MRKAIDTVKENSANLQIMKQNFERQLKEQQTQRLTSNTDAEKIGAKINQLEERMQRLSRTMESVE", "aa_hexdigest": "039a7f886538e5d6573fdcfaf0014f14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7666.626500000002, "isoelectric_point": 9.297517585754395 }, "id": "GIHJHMOALN_683", "locus": "GCA009939745_00656" }, { "type": "cds", "sequence": "contig_1", "start": 363595, "stop": 363774, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATGTTTGAAAAGGTATTAATGATGAACTTACTGCGCAGCACCACTCCAGTCCAACCTCCAGTGCAGTACTTTACTCCTCAACCCCTTACAGCATCGCCACGTCCAGTGTATGAAGAACCGATCTCCGCTTCGTTCCGCCGTCGTGGTTACCGTGGTGATGAATTTTACGAATAA", "aa": "MSMFEKVLMMNLLRSTTPVQPPVQYFTPQPLTASPRPVYEEPISASFRRRGYRGDEFYE", "aa_hexdigest": "16f35960ec594689398f294edc8c290c", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6943.915500000001, "isoelectric_point": 8.140503501892091 }, "id": "GIHJHMOALN_684", "locus": "GCA009939745_00657" }, { "type": "cds", "sequence": "contig_1", "start": 363794, "stop": 364207, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATACTGACAAGAAGTTCCCCTTGCAACCAGTTTATGACACTGGGTTTGATGATGGGTATTTACAGCGTGATTATGAAAAGCGTTTAGAAAGTGCTGCTGCTAATGATGCACAAACAGTGGAACTACAAACCCAGCTGTTAGCAGAGATTAAAAACCTTGAAAACGAAATCAAGGCCCTAAAGGCGCAGGAATCACGCCAACCCGATCCGCACAACAATGCCCGGATTCAAAGCTTAGAAGCTAGCTTAAACCGTTTGGTCAATGAGTACAACAACTTTGAATTCCAAAAGAACTATATGGTTGATCGGGTAGCAGAGTTAAATAACAAAGCGCGTTTTTTTAAGGACGAATTAAAGCGTCTCCAACAAGAAAACGCCGCTTTTGTTAATTCCCGTTATGCCAATTGA", "aa": "MNDTDKKFPLQPVYDTGFDDGYLQRDYEKRLESAAANDAQTVELQTQLLAEIKNLENEIKALKAQESRQPDPHNNARIQSLEASLNRLVNEYNNFEFQKNYMVDRVAELNNKARFFKDELKRLQQENAAFVNSRYAN", "aa_hexdigest": "b4229804fecd50ef046c4e9a4b826547", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16034.536799999983, "isoelectric_point": 5.114622688293457 }, "id": "GIHJHMOALN_685", "locus": "GCA009939745_00658" }, { "type": "cds", "sequence": "contig_1", "start": 364448, "stop": 364570, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCATCGAGTATGAAGCCACATACCAGGACTTGGTGAGTGCGGATGCGGAGTTGCAAAACGTTTATGAAACAATTGCCCAAAACCAGGCCAACTTCCAAAAGCAGTGTGATGCTTATTGA", "aa": "MGIEYEATYQDLVSADAELQNVYETIAQNQANFQKQCDAY", "aa_hexdigest": "255ce307c715f42848a3b54350a7048e", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4576.8905, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_686", "locus": "GCA009939745_00659" }, { "type": "cds", "sequence": "contig_1", "start": 364619, "stop": 365515, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGATGAAGAATCGGCACTTAAGGTGCGTTTAAATGACGCTGATTTTTACATTAACTCACGCTTAGCAGAATTAGATGATTTAACTAGCAAGATTAACGAACGTGATTTTGTTTCTAAAGAACAAGCTCAAGACGTTAAAGCTAGTTTAGCTAACCTCACTAAGGAAAAAGAACGTTTGAGCGCTGAGAAGGATAGCTTTGAAAGGTTAAGGAACACCGCTTTAAACGACATTAACCGAATGGAGCAGGAAAACGCGTTGTTTGCTAAACACTTAGAACAGCAACAATACGAGTTTGAAAGAAAGCAACAGGAAAGCTTATTAAAGCTAGAAACAGAGCATAAACAGCTCCAAAAACGAATTGGTGAGTTCAAAATCGAGAGTGAAGCTAAAAGTGAAGCGTTACTGATACAAGAACGTGAACTGTTGGAAAAACGCCGGGAAATTGATGATTTGTTAACCCAAGCCAGCTTGGAGTATGAACAACAACGCCAGACGAATCAGGTCTTAAAGGAAAAGCACCGTCAAGTCCAACAACACTTCCAAAACCTCGTGCATGCTAAAAAGAAATTGGATCAAAAGCGCCATTATTTAGCGGAACAAAAGCGGATTGATGAAGAACAGATTTTTAAGTTAAAGGAAAAGATTGCCACTGAACGCCGCGAATTGGAGAAGCTTTATCTAGTTAAAAAACAAAAGCAAGACCAAAAGGAAAACGATTTACTGATTTTTGAAAAACAGTTAAGGCAGTACCAAGCTGACTTTGAAAACGAAATAGAAGAGAAGCAAAACGAGCTGTTTGCATCCCAAAAATCACTCCAAAAATCGTTTACACAACTCAAAAATAAGGAAGCGGAATTAAACCAAAAGGCACAAAAGATTGCAGAGGATTGA", "aa": "MVDEESALKVRLNDADFYINSRLAELDDLTSKINERDFVSKEQAQDVKASLANLTKEKERLSAEKDSFERLRNTALNDINRMEQENALFAKHLEQQQYEFERKQQESLLKLETEHKQLQKRIGEFKIESEAKSEALLIQERELLEKRREIDDLLTQASLEYEQQRQTNQVLKEKHRQVQQHFQNLVHAKKKLDQKRHYLAEQKRIDEEQIFKLKEKIATERRELEKLYLVKKQKQDQKENDLLIFEKQLRQYQADFENEIEEKQNELFASQKSLQKSFTQLKNKEAELNQKAQKIAED", "aa_hexdigest": "253137fb854deee897549680b74679a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35790.806600000054, "isoelectric_point": 5.902581596374512 }, "id": "GIHJHMOALN_687", "locus": "GCA009939745_00660" }, { "type": "cds", "sequence": "contig_1", "start": 365609, "stop": 368677, "strand": "+", "frame": 2, "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0020035", "SO:0001217", "UniRef:UniRef50_P75471", "UniRef:UniRef90_P75471" ], "nt": "TTGGAAGCACGTACGCAGTTTGACAACCGTGTCAGTTTGCTCTCAGCACGCTTTAAACAAAAACAAGCAGAATTAGTTAAACAAAAACAAAGCTTGGAACAACTCACCGCTGCCTTTAATAAAGAGCAGGAAGCGGTAGAGCGTGATTGGAAGGATCGCTTAGCTAACTTAGAAAAGCAAAAGGAAATGCTGGGGGATAAGGTACACCAGTTTGATGAAAATTCGCTCAATATTTCCAAAAAGCTAGCGGAGCGGGAGCTAGCGATTAAATTTAAAGAAAAGGAGCTGGAGGCTGCACAAAAACAGCTAAGTTTAGACAACAACAATAACGCTGGGTTAAAGTTGCAGTTAGATAAATTGAGCGAATCACTCAAAACGGAACGCCTGGAATTAGAAGCATCTAAAGAACGTATTTTGGATTTTTACGATGAATCATCACGCCGAATTGCAGATTATGAAAGCGATTTACAAGCACGCTTAGCTGAGGTTAAGACACTAGAAAAAAACCAGCAGGAAACAGCGGCTAAAAGTGAGCGCGAACTCAAAGTAGCCTTAGAAAAGTTAAACCAAGCAAAAAAGGCTTTTTTACAAATTCGCAAACAGCAATTGCTCGAAATTGCTTCTGTAAAACAACAACTAGCACAAAAAGCTAATTTGTTGAAAAACCAACAAGCAGAGCTAGATAAGCAAACAGAAGAGTTAGAAGCAGCTTTTTTAGAGCAAGATACGGACAAAAAGGAGTTGGAAAAAGCACTCCACAGCGTTAAGTCCAAGCAAGAATTATTAGAGCGCGAACGCAGTTTTTTACTGCAAAAACAACGTGAATTTGCCGAACATGTAGCCGGCTTTAAGCGCCAAGTGCACTTTAAAACAACGCAAATGCAGCGTTTAAGTGAGTTCAACAAACAACAACAAAGTGAACAGATAAAGCGCGAAACGGAGCTCAAAATTGCTTTTGCTGATCTAAAAAAGGATTACCAGCTGTTTGAATTGCAAAAAAACCAGGAGTTCCAGCAAATCGAACAAAAGCACAAAGAACTAGAACTATTAGCACAAAAACAGGCCGAACTCAAACAGGAGTTAGAGCAAAAAGCAACAGCTTTAGCTAGCCAAGATCAAGACACAGTACAGGCCAAGCTAGATTTAGCGCGTCAACAGCATGAATTGGAATTGAAGCAAAATGCCTTTAACCAAGCTAGTCTTTCGCTCAACAAACAACGCGAACAGCTCACAAACCAAGTCAAGGTATTGCACGGTGAGTTGAAAAAACGCCATGAAAAGCTAACTTTAAAGGATCGTTTACTAGCTGAAAAGGAAAAGGATCAGCACAAAAAAGATGCAGAAATTAACCAACGCTTTAAGCAGTTTGAAAATGAATACGCTGATTTTGACCAAGCCAAAAAGCGCGAATTGCAAGAGTTAAACCAAATCCGTCGAAACTTGGAGCAGAGCAATGCATCACTGCTAAAGAAGCGCAACCAACTAACGCTTGATTTTGCTTTACTACGTAAAGTGCAACACAATACGCAAACTAACCGTGTCCAACTCAACACGCAAATTAAGGAGTTTTTACTAGAGAAGAAGAACTTTCAAAAGGCGAGTGATGAAGCCGCTTTACAAAAAGCACTGTTAATTAAACGCTTACGTAGTTTTGCTTCTAAATTACAGTTGCAACGCGAAGCTTTAGCGATTCAAAAACTGGAATTTGATAAGCGTGATGAACAACAAAAGAGTGAGATTAACAACGCTAAGTTACAGTTAGAGCAGTTCAAACTAGAAAAGCAGAACTTTGATGAAGCGAAACAAAAGCAGTTAATTGAGTTTAAGGATCAGTGCCAACGACTTGATGTTGAAAAACGTCTGTTAAAGCAAAAGTTAGTGCAGCTCAAAAACCTATCTAAGAGCTATCTTACTTACAAAAACCGTGCTGATCTGTCCCAACAACAGCTGCAACACAAGTATGCTAACTTACTGGAGTTGAAGGAAAAGCTGCAAACCGCTAAGCGTGCCTTGGACAAAAAACACCGTGCCATTTATGGCAAAATGGCACAGTTTGTCAGTGAGCTCCGCCAAGAAAAGAAGCAGCTTTTAAGTGCGCAAAAACAAGTTGATGACAAGTCACGTTTACTGGAACAAAACCAACGCCATTTACAAAACCTTTCCAGTGAAACCAAAAAGAAACGCCAGTCTTTAGAGCATGACATTAATAAGTTTGATCAAAGACGCAAGGAAGCGGTTTCTTCCATTTTAAACTCGCACAAAAAACTCAAGCAAAAAGAGGGAGAATTGCAAGGCATTTTGCAAAAGCTTAGCTTGAAAAAAACACAAATTGAACAAGAGTTTTCCAAGCTTTATCAGCAACGCGAAAAGTTAGATCGCCAACGCACTACGCTCTCTAAACTCCACAGAGAGCTAAAAGCCCAAAATGAAGCTACGGCACACAAAAATCGTGAAGTTTTAGAGATTGAGAACTATTACAAGAAGGAGTTGCAACGACTCACCACGGAAAAGAGTGAGTTTGATAACAACAAAAATCGCTTGTTTGAATACTTCCGTAAAATTCGCAATGAAATTGAAAAGAAGGAAGCACACATTAAAACCGTTTTAGAAGAAACACAAAAAAAGCGTCACCTAGTCGAAACGGAAGCGGTCAAACTACATTTACAAAAGCAATCTATCATTTCTAAGGGTCAAGAACTTAAAGAAATCAAGGAACGGGTTAGCCGTGACATTAGCCACACCAACAAACAACGCGAAGAGTTAAACAGCTTATTGCACCAAAACAAACTGTTGCAAAAGAATTTGGCTGAGCGTGAACGTGAAATTAATAATAAGGATTCACTGTTAACCCAAAAGATTCAAACAGCTAAACAAAAGTTAAGCGAAAAAGAAGCACGCATCTTAAAGCTGTTGGAAAAAATGCGTGCGGTAGAACAGCAATACCAAGCGGAAATTACCCGTTTAAAGACACGTAATGCTGATTTGGAGAAGAATGACAATAAGCACTTATTTCCACCGTTATTTAAAATTAACGGTAATGATATGAACTATCCTTACCCATATCCTTGA", "aa": "MEARTQFDNRVSLLSARFKQKQAELVKQKQSLEQLTAAFNKEQEAVERDWKDRLANLEKQKEMLGDKVHQFDENSLNISKKLAERELAIKFKEKELEAAQKQLSLDNNNNAGLKLQLDKLSESLKTERLELEASKERILDFYDESSRRIADYESDLQARLAEVKTLEKNQQETAAKSERELKVALEKLNQAKKAFLQIRKQQLLEIASVKQQLAQKANLLKNQQAELDKQTEELEAAFLEQDTDKKELEKALHSVKSKQELLERERSFLLQKQREFAEHVAGFKRQVHFKTTQMQRLSEFNKQQQSEQIKRETELKIAFADLKKDYQLFELQKNQEFQQIEQKHKELELLAQKQAELKQELEQKATALASQDQDTVQAKLDLARQQHELELKQNAFNQASLSLNKQREQLTNQVKVLHGELKKRHEKLTLKDRLLAEKEKDQHKKDAEINQRFKQFENEYADFDQAKKRELQELNQIRRNLEQSNASLLKKRNQLTLDFALLRKVQHNTQTNRVQLNTQIKEFLLEKKNFQKASDEAALQKALLIKRLRSFASKLQLQREALAIQKLEFDKRDEQQKSEINNAKLQLEQFKLEKQNFDEAKQKQLIEFKDQCQRLDVEKRLLKQKLVQLKNLSKSYLTYKNRADLSQQQLQHKYANLLELKEKLQTAKRALDKKHRAIYGKMAQFVSELRQEKKQLLSAQKQVDDKSRLLEQNQRHLQNLSSETKKKRQSLEHDINKFDQRRKEAVSSILNSHKKLKQKEGELQGILQKLSLKKTQIEQEFSKLYQQREKLDRQRTTLSKLHRELKAQNEATAHKNREVLEIENYYKKELQRLTTEKSEFDNNKNRLFEYFRKIRNEIEKKEAHIKTVLEETQKKRHLVETEAVKLHLQKQSIISKGQELKEIKERVSRDISHTNKQREELNSLLHQNKLLQKNLAEREREINNKDSLLTQKIQTAKQKLSEKEARILKLLEKMRAVEQQYQAEITRLKTRNADLEKNDNKHLFPPLFKINGNDMNYPYPYP", "aa_hexdigest": "554e862b292ba705b0d4e11583b148cd", "start_type": "TTG", "rbs_motif": "AACAA", "genes": [ "hmw2" ], "truncated": "both", "pseudogene": { "start": 365309, "stop": 368980, "observations": { "mutation": [ 365513, 368675 ], "stop": [ 365513, 368675 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75471", "query_cov": 1.0, "subject_cov": 0.5621562156215621, "identity": 0.985, "score": 1639.0, "evalue": 0.0, "gene_start": 1, "gene_end": 1022, "reference_start": 606, "reference_end": 1627, "reference_sequence": "MNDTDKKFPLQPVYDTGFDDGYLQRDYEKCLESAAANDAQTVELQTQLLAEIKNLENEIKALKAQESRQPDPHNNARIQSLEASLNRLVNEYNNFEFQKNYMVDRVAELNNKARFFKDELKRLQQENAAFVNSRYANWADFQSNYQLKLDQFQALIDQQNQTIKQLNEQIAANQGLIDQNVQRLQQNHSLDQQERDALLYEVDHLYNELYELENQKRLVGIEYEATYQDLVSADAELQNVYETIAQNQANFQKQCDAYWAQLKQVEQQIQTTKQELVDEESTLKVRLNDADFYINSRLAELDDLTSKINERDFVSKEQAQDVKASLANLTKEKERLSAEKDSFERLRNTALNDINRMEQENALFAKHLEQQQYEFERKQQESLLKLETEHKQLQKRIGEFKIESEAKSEALLIQERELLEKRREIDDLLTQASLEYEQQRRTNQVLKEKHRQVQQHFQNLVHAKKKLDQKRHYLAEQKRIDEEQIFKLKEKIATERRELEKLYLVKKQKQDQKENDLLIFEKQLRQYQADFENEIEEKQNELFASQKSLQKSFTQLKNKEAELNQKAQKIAEDWAHLKQNKHHHADLEIFLEGEFNHLQQEKHKLLEARTQFDNRVSLLSARFKQKQAELVKQKQSLEQLTAAFNKEQEAVERDWKDRLANLEKQKEMLGDKVHQFDENSLNISKKLAERELAIKFKEKELEAAQKQLSLDNNNNAGLKLQLDKLSESLKTERLELEASKERILDFYDESSRRIADYESDLQARLAEVKTLEKNQQETAAKSERELKVALEKLNQAKKAFLQIRKQQLLEIASVKQQLAQKANLLKNQQAELDKQTEELEAAFLEQDTDKKELEKALHSVKSKQELLERERSFLLQKQREFAEHVAGFKRQVHFKTTQMQRLSEFNKQQQSEQIKRETELKIAFADLKKDYQLFELQKNQEFQQIEQKHKELELLAQKQAELKQELEQKATALASQDQDTVQAKLDLARQQHELELRQNAFNQASLSLNKQREQLTNQVKVLHGELKKRHEKLTLKDRLLAEKEKDQHKKDAEINQRFKQFENEYADFDQAKKRELQELNQIRRNLEQSNASLLKKRNQLTLDFALLRKVQHNTQTNRVQLNTQIKEFLLEKKNFQKASDEAALQKALLIKRLRSFASKLQLQREALAIQKLEFDKRDEQQKSEINNAKLQLEQFKLEKQNFDEAKQKQLIEFKDQCQRLDVEKRLLKQKLVQLKNLSKSYLTYKNRADLSQQQLQHKYANLLELKEKLQTAKRALDKKHRAIYGKMAQFVSELRQEKKQLLSAQKQVDDKSRLLEQNQRHLQNLSSETKKKRQSLEHDINKFDQRRKEAVSSILNSHKKLKQKEGELQGILQKLSLKKTQIEQEFSKLYQQREKLDRQRTTLSKLHRELKAQNEATAHKNREVLEIENYYKKELQRLTTEKSEFDNNKNRLFEYFRKIRNEIEKKEAHIKTVLEETQKKRHLVETEAVKLHLQKQSIISKGQELKEIKERVSRDISHTNKQREELNSLLHQNKLLQKNLAEREREINNKDSLLTQKIQTAKQKLSEKEARILKLLEKMRAVEQQYQAEITRLKTRNADLEKNDNKHLFPPLFKINGNDMNYPYPYPWFYPQQKQEDSSNQIRHLFEQQLQFMQQRYENELTELRRQRALLEKKLDQIQLESQLSAKKNDFEKVEQMMQKLLEKTEQKLSAFDQKINALAEQINTQKAEHADSEKQQLLLRIEQLEKQNLAQAVQTPQPVQPVVQAPAVVPQVIQPQVVQSQPAFLATQQSISKQQQIAQLNAEINSIKKLIAQKAAK" }, "paralog": false, "identity": 0.9918233851185609, "score": 2163.0, "evalue": 0.0, "description": "Internal stop codon at 365513, 368675. Nonsense mutation around 365513, 368675.", "psc": { "uniref90_id": "UniRef90_P75471", "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "uniref50_id": "UniRef50_P75471", "cog_id": "COG1196", "cog_category": "D", "go_ids": [ "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75471", "UniRef:UniRef50_P75471", "COG:COG1196", "COG:D", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_P75471", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75471" ], "product": "Cytadherence high molecular weight protein 2" } }, "id": "GIHJHMOALN_688", "locus": "GCA009939745_00661" }, { "type": "cds", "sequence": "contig_1", "start": 368750, "stop": 369250, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAGCGTTACGAAAACGAATTAACTGAGTTGCGTCGCCAACGTGCTTTATTGGAAAAAAAACTAGACCAAATTCAACTCGAATCACAGTTAAGCGCGAAGAAGAACGACTTTGAAAAGGTTGAGCAAATGATGCAAAAGTTGTTGGAAAAAACTGAGCAAAAACTCAGTGCCTTTGACCAAAAAATTAATGCTTTGGCAGAACAGATTAACACACAAAAAGCAGAGCACGCTGACAGTGAAAAGCAACAGTTGTTACTAAGGATTGAACAGTTAGAAAAGCAAAACCTAGCTCAAGCTGTTCAAACACCCCAACCAGTACAACCAGTGGTACAAGCACCAGCTGTAGTACCCCAAGTGATCCAACCACAGGTAGTGCAGTCCCAACCAGCTTTTTTAGCAACGCAGCAAAGTATTTCTAAACAGCAACAAATCGCGCAATTAAACGCTGAGATCAATAGTATTAAAAAGCTAATTGCCCAAAAAGCAGCTAAATAA", "aa": "MQQRYENELTELRRQRALLEKKLDQIQLESQLSAKKNDFEKVEQMMQKLLEKTEQKLSAFDQKINALAEQINTQKAEHADSEKQQLLLRIEQLEKQNLAQAVQTPQPVQPVVQAPAVVPQVIQPQVVQSQPAFLATQQSISKQQQIAQLNAEINSIKKLIAQKAAK", "aa_hexdigest": "92065eea48fde9f1034a1024184f916b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18971.563099999985, "isoelectric_point": 9.097601127624515 }, "id": "GIHJHMOALN_689", "locus": "GCA009939745_00662" }, { "type": "cds", "sequence": "contig_1", "start": 369265, "stop": 370338, "strand": "+", "frame": 1, "gene": null, "product": "putative MG218-1-like protein", "db_xrefs": [ "RefSeq:WP_014325454.1", "SO:0001217", "UniParc:UPI0001DD6523", "UniRef:UniRef100_A0AAV5N9F2", "UniRef:UniRef50_Q9ZB78", "UniRef:UniRef90_P75470" ], "nt": "ATGACTAATGATTACCAACAATTAAATTATTTAGTGGAAACTGATGATGAAGCTGACATCATCATAGCTAACCTCGTCAAACAACTCAATGAATTGAAGGAAATTCTGCTTTCATTGGACAATCAAGATTTAGAAATTAATCAGGTTAACCATCGCACCACAGTGCACAATACTAGTTCTAACACTTCTAATAATAGCACTAGCAATAATAGTGCTGATGGTAATTACAACAGCAATTTCTTTCACAACTTTAGTAAAGAAACGCTGCAGACACAAGCAAAACGTGGCTTTCTTTTACTAGAACGCTGTTCCTTGGTAGGTCTACAACAATTGGAGTTGGAATACCTCAACGTATTGGGTCGTAGCTTTGAATCACATCAAGACAAAATTAACTTTTTGGTCAATTTACGTGATTTAACTAACGATCACTTGTTGGACACGGAAAAGATTGTCAGTACCCTAGAGCAGATCTTTAACGTTATTGGTGGCACGGAATACACCCCGATTCTCAATTCCTTTTTTAACCAATCACTTAACGATCCTGACCCAATTCAGCGCGAAATTGGACTCAAACAGTTTGTGGCTAACTTACGCCAACGCTTTAAAACGCTATTGCAAAAAACTAACAACTCAATTCAACAGTTAGAAGCGGAAATTCAAATTCCAACTACTCATATTAAGAGTGATGAGGTAATGTTTGGCCCACCGGACATGAACGAACGCTTAGTGTTAAATGACAGTGAAACCGATGCCATTCTACGCTCAATTGAAGCGGAGTTGGAAAGCGCTTTACAAAATAAAAAGCAGGTAGTGGTTACAGCAGTCCCACCAATAGCTGCTAACTTAGCTACCGATAGTATTAGCCAAGAAACTTTAGAACACAACCTTAATAACACTGAAACGGTTAACACCACTACTGTCACGGTAACATCAGCTAGTGAAACTCAAGTGCGTAAACCACAGATTTCATTACGCCCCATTTTTCAAGGCAACTTTCCCAAACGCTTAAGTAGAGAAGACATCCAGCGCTATGCCCACCAGTTACAGGAATTAGAACAAAGTAATGAAGGATAG", "aa": "MTNDYQQLNYLVETDDEADIIIANLVKQLNELKEILLSLDNQDLEINQVNHRTTVHNTSSNTSNNSTSNNSADGNYNSNFFHNFSKETLQTQAKRGFLLLERCSLVGLQQLELEYLNVLGRSFESHQDKINFLVNLRDLTNDHLLDTEKIVSTLEQIFNVIGGTEYTPILNSFFNQSLNDPDPIQREIGLKQFVANLRQRFKTLLQKTNNSIQQLEAEIQIPTTHIKSDEVMFGPPDMNERLVLNDSETDAILRSIEAELESALQNKKQVVVTAVPPIAANLATDSISQETLEHNLNNTETVNTTTVTVTSASETQVRKPQISLRPIFQGNFPKRLSREDIQRYAHQLQELEQSNEG", "aa_hexdigest": "944df2fbb3876d7242ffc8a48bc88855", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI0001DD6523", "ncbi_nrp_id": "WP_014325454.1", "uniref100_id": "UniRef100_A0AAV5N9F2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6523", "RefSeq:WP_014325454.1", "UniRef:UniRef100_A0AAV5N9F2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N9F2", "uniref90_id": "UniRef90_P75470" }, "psc": { "uniref90_id": "UniRef90_P75470", "product": "Uncharacterized protein MG218.1 homolog", "uniref50_id": "UniRef50_Q9ZB78" }, "pscc": { "uniref50_id": "UniRef50_Q9ZB78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB78" ], "product": "Uncharacterized protein MG218.1" }, "genes": [], "id": "GIHJHMOALN_690", "locus": "GCA009939745_00663" }, { "type": "cds", "sequence": "contig_1", "start": 370328, "stop": 370882, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75469", "UniRef:UniRef90_P75469" ], "nt": "ATGAAGGATAGTGCACTAACACTAAAACGGGTACGAATTGGAAAATTTTCCGAGTCAATGGTTGAAGAACGACCAACACTCAACCTGTTCGAAAAGGTCGAATTCAACCCAGTACCGACTGCTTTAGTGGATCAATTGCCAACTGAACCACTTGTTGAAGCAACTCTTTTAGAAAAGGAAGCAATTACTTTTGTCGATACTTACGCAACTTCGGAAGCTCATGATCAAATAGCTACCTTTGTTTTAGAACAATCAATGGAAACGGAGGTGGTTGAAAAAGAAGCGATTGAAACAGCAATAGTTGCACCAGCACCAGACTTAGTTGAAGAGAAAGCTGTTTTAGTGGAAGAGGTTTTAGTTGAACCAACTGCAACGGAAGCAGTTACAACTGAAGAAAATCAAGTTTCAACTACATCCGTAACTAAGATTAAAACTAAGCGCAGCAATACTAAAAAGGTGACATCAGAAACACTTGTGGCTTCTAAAAGTGTGAAAACTAAAAAGTTAATTACCCCTAACCGGGTAAGTTCGGGCAACGTTAATATAACACTTTGA", "aa": "MKDSALTLKRVRIGKFSESMVEERPTLNLFEKVEFNPVPTALVDQLPTEPLVEATLLEKEAITFVDTYATSEAHDQIATFVLEQSMETEVVEKEAIETAIVAPAPDLVEEKAVLVEEVLVEPTATEAVTTEENQVSTTSVTKIKTKRSNTKKVTSETLVASKSVKTKKLITPNRVSSGNVNITL", "aa_hexdigest": "5b386ea693dd4068c2a82f5e25a6b8e8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75469", "query_cov": 1.0, "subject_cov": 0.8440366972477065, "identity": 1.0, "score": 270.0, "evalue": 1.55e-89, "valid": true, "product": "Uncharacterized protein MPN_312", "uniref50_id": "UniRef50_P75469" }, "pscc": { "uniref50_id": "UniRef50_P75469", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75469" ], "product": "Uncharacterized protein MPN_312" }, "genes": [], "id": "GIHJHMOALN_691", "locus": "GCA009939745_00664" }, { "type": "cds", "sequence": "contig_1", "start": 371067, "stop": 371261, "strand": "-", "frame": 2, "gene": null, "product": "MPN313 family protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniParc:UPI0001DD6525", "UniRef:UniRef100_P75468", "UniRef:UniRef50_Q49403", "UniRef:UniRef90_P75468" ], "nt": "ATGATTGTAAACGCCTTTAGTACGTTAATTAATACCGCGTTAGACATCAGCGAAGCTGCTAACGCTAATAATGCCAACGGCAATGGCAGCAGTTATAGCTATAAAAGAAGGAATTCACAAAAAGACTACTTTAGTACCGGTCGGTTCCGATTAGGTTTAACCCCTGGTAAATCGTCCTATTCTTTTCCCGTTTAA", "aa": "MIVNAFSTLINTALDISEAANANNANGNGSSYSYKRRNSQKDYFSTGRFRLGLTPGKSSYSFPV", "aa_hexdigest": "faaba2bcf9aacc473b9113f13bb76ee0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6525", "uniref100_id": "UniRef100_P75468", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6525", "UniRef:UniRef100_P75468" ] }, "ips": { "uniref100_id": "UniRef100_P75468", "uniref90_id": "UniRef90_P75468" }, "psc": { "uniref90_id": "UniRef90_P75468", "product": "MPN313 family protein", "uniref50_id": "UniRef50_Q49403", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q49403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49403" ], "product": "Uncharacterized protein MG220" }, "genes": [], "id": "GIHJHMOALN_692", "locus": "GCA009939745_00665" }, { "type": "tRNA", "sequence": "contig_1", "start": 371409, "stop": 371485, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(gcg)", "amino_acid": "Arg", "anti_codon": "gcg", "score": 65.2, "nt": "GTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 371443, 371445 ], "id": "GIHJHMOALN_9", "locus": "GCA009939745_00666" }, { "type": "cds", "sequence": "contig_1", "start": 371740, "stop": 372165, "strand": "+", "frame": 1, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143", "RefSeq:WP_010874670.1", "SO:0001217", "UniParc:UPI000012F547", "UniRef:UniRef100_P75467", "UniRef:UniRef50_P47463", "UniRef:UniRef90_P75467" ], "nt": "ATGCTTTTAGGAACCTTCAACATTACCCTTGATGCAAAAAACAGGATCAGTCTGCCAGCGAAGCTGCGGGCTTTCTTTGAAGGTAGCATTGTTATCAACCGTGGCTTTGAAAACTGCTTGGAAGTGCGTAAACCACAAGACTTCCAAAAGTACTTTGAGCAGTTCAACAGCTTTCCCTCCACCCAAAAAGACACTAGAACACTCAAGCGCTTAATTTTTGCCAACGCTAACTTTGTGGATGTAGATACAGCGGGACGGGTTTTAATTCCCAACAATCTGATTAACGATGCCAAGCTAGACAAGGAAATTGTTTTAATTGGCCAGTTTGACCACTTAGAGATTTGGGACAAGAAGCTGTATGAGGACTACCTAGCCAACTCTGAGTCGTTGGAAACGGTAGCGGAAAGGATGAAAGATGTTAAGTAA", "aa": "MLLGTFNITLDAKNRISLPAKLRAFFEGSIVINRGFENCLEVRKPQDFQKYFEQFNSFPSTQKDTRTLKRLIFANANFVDVDTAGRVLIPNNLINDAKLDKEIVLIGQFDHLEIWDKKLYEDYLANSESLETVAERMKDVK", "aa_hexdigest": "8b2975df80e77adad7ccac97fe29d2f1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012F547", "ncbi_nrp_id": "WP_010874670.1", "uniref100_id": "UniRef100_P75467", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012F547", "RefSeq:WP_010874670.1", "UniRef:UniRef100_P75467" ] }, "ips": { "uniref100_id": "UniRef100_P75467", "uniref90_id": "UniRef90_P75467" }, "psc": { "uniref90_id": "UniRef90_P75467", "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "uniref50_id": "UniRef50_P47463", "cog_id": "COG2001", "cog_category": "J", "go_ids": [ "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143" ] }, "pscc": { "uniref50_id": "UniRef50_P47463", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47463" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "GIHJHMOALN_693", "locus": "GCA009939745_00667" }, { "type": "cds", "sequence": "contig_1", "start": 372155, "stop": 373081, "strand": "+", "frame": 2, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424", "RefSeq:WP_014325456.1", "SO:0001217", "UniParc:UPI0001DD6526", "UniRef:UniRef100_A0AAV5NAY0", "UniRef:UniRef50_P47464", "UniRef:UniRef90_P75466" ], "nt": "ATGTTAAGTAATCAACCCCACAAAAGTGTCTTACTTGATGAAGTCATCCACAACCTCAACATTAAAGGTGATGGTAACTATTTAGATCTTACTGTTGGTTTTGGCGGTCATAGTCAGCACATTTTAGAGCAGCTTACCACTGGCACTTTAACTGGCAATGACATGGATAAGGACAGCATTACCTTTTGTACCGAGCTGTTCAAAGACAAAAAGAACGTTGTTTTAGTCCATGATAACTTCGCTAACTTTTTTAACCACTTAAAGCAGCTCAAAGTAACGAAGTTTGACGGCATCTTAATTGATTTAGGGGTTTCGAGCTACCAGCTAGATAAACCAGAACGCGGTTTTAGTTTTAAACACGCCGGTCCATTTGACATGCGCATGCACCAAAGCGATCGCGTACCAACTGCTTTAGATATCTTGGAGAGGTTGTCTGAGGAAGAATTGACACATGTTTTAAAGAAGTTTGGTGAAATAGCCCACCCCAAACCAATCGCCAAGGCCTTAAAAACACTCATTAATAACATTAAAAATCCAACTACGGTAGAGGTAGCGGAAACAGTTAAGGCAGCTGCTAGCAACTTTGAAAAGTACAAATCACGCAATTACTTAGCTAAAGTTTTCCAAGCCATTCGCATTTACCTCAACCGTGAACTGGAAAGTTTGGAAATTGTGCTGCAACACATTCCCAAGCTGTTGAACAATAAGGGTCGTTTTCTAGTGATTGTCTTCCATTCACTCGAGGAAAAATTGGTGCGGAACTACATTTTTAAACTTACTCACTTTGTCCAACCCCCTGAACTACCCGTTAAATTAACCCCACCGTTTGAACTCATTACTAAAAAACCCATTTTGCCAACTGAACAAGAAATTAAAACGAACCCCCGTGCTCGAAGTGCCAAACTGTTTGTCATTGAAAAGAAATAA", "aa": "MLSNQPHKSVLLDEVIHNLNIKGDGNYLDLTVGFGGHSQHILEQLTTGTLTGNDMDKDSITFCTELFKDKKNVVLVHDNFANFFNHLKQLKVTKFDGILIDLGVSSYQLDKPERGFSFKHAGPFDMRMHQSDRVPTALDILERLSEEELTHVLKKFGEIAHPKPIAKALKTLINNIKNPTTVEVAETVKAAASNFEKYKSRNYLAKVFQAIRIYLNRELESLEIVLQHIPKLLNNKGRFLVIVFHSLEEKLVRNYIFKLTHFVQPPELPVKLTPPFELITKKPILPTEQEIKTNPRARSAKLFVIEKK", "aa_hexdigest": "847daf484b3924d2245557a8b918bf52", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6526", "ncbi_nrp_id": "WP_014325456.1", "uniref100_id": "UniRef100_A0AAV5NAY0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6526", "RefSeq:WP_014325456.1", "UniRef:UniRef100_A0AAV5NAY0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NAY0", "uniref90_id": "UniRef90_P75466" }, "psc": { "uniref90_id": "UniRef90_P75466", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_P47464", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424" ] }, "pscc": { "uniref50_id": "UniRef50_P47464", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47464" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "GIHJHMOALN_694", "locus": "GCA009939745_00668" }, { "type": "cds", "sequence": "contig_1", "start": 373084, "stop": 374199, "strand": "+", "frame": 1, "gene": null, "product": "MPN316 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47465", "UniRef:UniRef90_P75465" ], "nt": "ATGTATAACCTCAAAAACATCTACGCCTCCATTACCCTTTACCCCCATGAAATTAACTTGGTGGTATCGGACAACAACAATCAGTTTCATGTCTTGTATGAAAACTCGATTGCTAACGATGAGCTTTACACGCACGCGGGCATTACTAATAAGGCAAAGTTCAAGGTAGTTTTAAACCAGCTCATTAACAATGCAAACGATTATTTGGGTTTTAAACTCGAAAAAGTAATTGTGGTTTTAGCGGAGTTAGTTGATGATCTTGAAATTAACCGCTTTAAAACAGATGTCTTTTTCACTGGTTATGACTTTAACCACCAGGACATGTTGAGCAGTGAAAAGAACCGCTTCTTGCTCAAAAACCGTCATATCAACCCCAATGAAGTTTTGGACTTAGTGGCTTTAAACTACCGTGATTTAACCACTGGTAAAATTAGCAAAAACTTTAAGTACAACTGCAGTTATCGGGCAAATGTAATTTATTACACCACTAAAAACTCACTAGTCAAGGAGCTCAAACCGTTTTTAAAGCGCAATATTATTGTCAAAATTGACAAAATTGTCACCCACCACATGGTGTTAGCCCACTCAATTAAACAAATCAACAAAAACAACCTCTTTGTGTATTTAGGGGAACACACTACTGACCTCATGTTGTTTATGGACAATGCCTTAGTTGATTTAGTAAGTGAACCCTTTGGTCGGGTTAACTTCTTGGAATCTGACAATGACAGTGAAAACAAAGCGCTGTTGGAATTCCTAGTGGACTCTACTGCACGCATTGGTGACTCGGGCTCGATTGGCATGGCTTACACCGATGGTTCTACTTATAAGGAAATTAGAGCTGTTACGATTGATGACCTCATTCAAAGTGTTAATGAAAAAACGAAGTACTTAATTGATTTCATTAACACTAACACGGAAATCTTCTTCCAAAAGTACGGCTTTTTACCCAGCACTTTACTGTTTTACACTAAGAGTAAACAGTTGCTTAACAACTTTCAACTCAACCAAAACCTGTTGAGTAACCACTTCAAAACTGTAACCATCTTTAAAAACGAAGTTCAGTTTGTGAGTCGCAAATACTTATTGAACTGTCAAGCAATTGCGCTTTCTTGA", "aa": "MYNLKNIYASITLYPHEINLVVSDNNNQFHVLYENSIANDELYTHAGITNKAKFKVVLNQLINNANDYLGFKLEKVIVVLAELVDDLEINRFKTDVFFTGYDFNHQDMLSSEKNRFLLKNRHINPNEVLDLVALNYRDLTTGKISKNFKYNCSYRANVIYYTTKNSLVKELKPFLKRNIIVKIDKIVTHHMVLAHSIKQINKNNLFVYLGEHTTDLMLFMDNALVDLVSEPFGRVNFLESDNDSENKALLEFLVDSTARIGDSGSIGMAYTDGSTYKEIRAVTIDDLIQSVNEKTKYLIDFINTNTEIFFQKYGFLPSTLLFYTKSKQLLNNFQLNQNLLSNHFKTVTIFKNEVQFVSRKYLLNCQAIALS", "aa_hexdigest": "1d06c9d7e5ad8a72784804a53d16e1cc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75465", "query_cov": 1.0026954177897573, "subject_cov": 0.8857142857142857, "identity": 0.997, "score": 723.0, "evalue": 2.2e-262, "valid": true, "product": "MPN316 family protein", "uniref50_id": "UniRef50_P47465" }, "pscc": { "uniref50_id": "UniRef50_P47465", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47465" ], "product": "Uncharacterized protein MG223" }, "genes": [], "id": "GIHJHMOALN_695", "locus": "GCA009939745_00669" }, { "type": "cds", "sequence": "contig_1", "start": 374454, "stop": 375245, "strand": "+", "frame": 3, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "COG:COG0206", "COG:D", "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258", "KEGG:K03531", "SO:0001217", "UniRef:UniRef50_P47466", "UniRef:UniRef90_P75464" ], "nt": "ATGTTGCGCATGCACCCCGAATTGCAAACGGCTAACGTCCAGTTCTTTGCTTTAAACACTGATTTACAGCACTTAAAAACAAAGCGCTATGTCCAAAATAAGGCTGTGATTCAGTTTGAAGAATCCAAGGGCTTAGGTGTTGGTGGTGATCCCCAAAAAGGTGCCGTTTTAGCACACCATTTTTTAGAGCAATTTCACAAACTTAGTGACAGCTTTGATTTCTGTATTTTAGTCGCTGGTTTTGGTAAGGGGACTGGTACTGGTGCTACCCCGGTTTTTAGTAAGTTTCTTAGCAACAAAGGGGTGTTAAATCTCTCGATTGTCAGTTACCCCGCAATGTGTGAAGGGTTAAAGGCACGCGAAAAAGCAGCTAAAGGTTTAGAACGCTTAAACCAAGCGACTGACAGTTTTATGCTGTTCCGCAATGATCGCTGTACTGATGGCATTTACCAACTAGCAAACGTTGCCATTGTCAAAACAATTAAAAACATTATTGAACTAATTAATCTACCACTACAGCAAAACATTGATTTTGAAGACATTCGTTCTTTCTTTAAAAAACCAGCACAACGTTTAGAGAATGAAGCTAACTTGTTTCGCGTAACTAATACTTTTACTTTTAGCTTTGACGCGCACAATACAATTGAGCACTTTAGCCACAAATTGAAAAACTTTGAGTATGAAGGTTTTTTTGACCATAAGGTAGAAGGGGCGCAAAAGGTAATTTTAAAGGTGTTAGTCAACCAAGGTTTATATCCGCTTGATTTAACCCAAATTCAAGAGATTATTTGA", "aa": "MLRMHPELQTANVQFFALNTDLQHLKTKRYVQNKAVIQFEESKGLGVGGDPQKGAVLAHHFLEQFHKLSDSFDFCILVAGFGKGTGTGATPVFSKFLSNKGVLNLSIVSYPAMCEGLKAREKAAKGLERLNQATDSFMLFRNDRCTDGIYQLANVAIVKTIKNIIELINLPLQQNIDFEDIRSFFKKPAQRLENEANLFRVTNTFTFSFDAHNTIEHFSHKLKNFEYEGFFDHKVEGAQKVILKVLVNQGLYPLDLTQIQEII", "aa_hexdigest": "bbc85d5f84792e73cad421505bb2db29", "start_type": "ATG", "rbs_motif": "AACAA", "genes": [ "ftsZ" ], "truncated": "both", "pseudogene": { "start": 374349, "stop": 375491, "observations": { "mutation": [ 374355, 375243 ], "stop": [ 374355, 375243 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75464", "query_cov": 1.0, "subject_cov": 0.6921052631578948, "identity": 1.0, "score": 524.0, "evalue": 2.57e-186, "gene_start": 1, "gene_end": 263, "reference_start": 36, "reference_end": 298, "reference_sequence": "MDWIQTAGAGTQLPENNIKIAVFGIGGAGNNIIDDMLRMHPELQTANVQFFALNTDLQHLKTKRYVQNKAVIQFEESKGLGVGGDPQKGAVLAHHFLEQFHKLSDSFDFCILVAGFGKGTGTGATPVFSKFLSNKGVLNLSIVSYPAMCEGLKAREKAAKGLERLNQATDSFMLFRNDRCTDGIYQLANVAIVKTIKNIIELINLPLQQNIDFEDIRSFFKKPAQRLENEANLFRVTNTFTFSFDAHNTIEHFSHKLKNFEYEGFFDHKVEGAQKVILKVLVNQGLYPLDLTQIQEIIWAKIDNHNLEVQLGVDFTDANPSVQLFFLMEKKQAVSSDFIQKPAFISVKEVNQKPAKPFQVLNDLKELGLKYVKQQTGFNY" }, "paralog": false, "identity": 0.9947368421052631, "score": 750.0, "evalue": 1.07e-277, "description": "Internal stop codon at 374355, 375243. Nonsense mutation around 374355, 375243.", "psc": { "uniref90_id": "UniRef90_P75464", "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_P47466", "cog_id": "COG0206", "cog_category": "D", "kegg_orthology_id": "K03531", "go_ids": [ "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75464", "UniRef:UniRef50_P47466", "COG:COG0206", "COG:D", "KEGG:K03531", "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258" ] }, "pscc": { "uniref50_id": "UniRef50_P47466", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47466" ], "product": "Cell division protein FtsZ" } }, "id": "GIHJHMOALN_696", "locus": "GCA009939745_00670" }, { "type": "cds", "sequence": "contig_1", "start": 375312, "stop": 375491, "strand": "+", "frame": 3, "gene": "ftsZ", "product": "Cell division protein FtsZ", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8D0", "UniRef:UniRef90_A0AB38W8D0" ], "nt": "GTGCAACTGTTTTTCTTAATGGAGAAAAAGCAAGCAGTGTCCTCTGACTTTATTCAAAAACCAGCATTTATCTCAGTTAAAGAAGTAAATCAGAAACCGGCCAAACCGTTCCAAGTTTTGAATGATTTAAAGGAGCTGGGACTGAAATACGTCAAACAGCAAACCGGATTTAATTATTAG", "aa": "MQLFFLMEKKQAVSSDFIQKPAFISVKEVNQKPAKPFQVLNDLKELGLKYVKQQTGFNY", "aa_hexdigest": "16a3b4888b1450bbcb56766c25c74535", "start_type": "GTG", "rbs_motif": null, "genes": [ "ftsZ" ], "truncated": "5-prime", "pseudogene": { "start": 375117, "stop": 375491, "observations": { "mutation": [ 375243 ], "stop": [ 375243 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8D0", "query_cov": 0.9830508474576272, "subject_cov": 0.46774193548387094, "identity": 1.0, "score": 115.0, "evalue": 7.25e-32, "gene_start": 2, "gene_end": 59, "reference_start": 67, "reference_end": 124, "reference_sequence": "MKNFEYEGFFDHKVEGAQKVILKVLVNQGLYPLDLTQIQEIIWAKIDNHNLEVQLGVDFTDANPSVQLFFLMEKKQAVSSDFIQKPAFISVKEVNQKPAKPFQVLNDLKELGLKYVKQQTGFNY" }, "paralog": false, "identity": 0.9838709677419355, "score": 243.0, "evalue": 6.28e-85, "description": "Internal stop codon at 375243. Nonsense mutation around 375243.", "psc": { "uniref90_id": "UniRef90_A0AB38W8D0", "product": "Cell division protein FtsZ", "uniref50_id": "UniRef50_A0AB38W8D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8D0", "UniRef:UniRef50_A0AB38W8D0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8D0" ], "product": "Cell division protein FtsZ" } }, "id": "GIHJHMOALN_697", "locus": "GCA009939745_00671" }, { "type": "cds", "sequence": "contig_1", "start": 375534, "stop": 375656, "strand": "+", "frame": 3, "gene": null, "product": "amino acid permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8", "UniRef:UniRef90_UPI0027DFE8A8" ], "nt": "ATGGCTGTTGCAAGTAAGAAGTTCACTGAACGTCAATTTTTATTTTTTGTTGTCAACTATATTGCTGGCTTTGGTTTTATTTCCACTGCCCTATCACTGTTTGAGCTAGGACCACTTTCATGA", "aa": "MAVASKKFTERQFLFFVVNYIAGFGFISTALSLFELGPLS", "aa_hexdigest": "4ff39760f5312f6112037f106ce43c56", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 375534, "stop": 375800, "observations": { "mutation": [ 375654, 375756 ], "stop": [ 375654, 375756 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0027DFE8A8", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 1.0, "score": 79.3, "evalue": 1.68e-18, "gene_start": 1, "gene_end": 40, "reference_start": 1, "reference_end": 40, "reference_sequence": "MAVASKKFTERQFLFFVVNYIAGFGFISTALSLFELGPLSWVIFFIVSLISLIVTLAFARVSAIDKNNYGGAYLWAKKAFNSERYSHR" }, "paralog": false, "identity": 0.9659090909090909, "score": 163.0, "evalue": 5.32e-54, "description": "Internal stop codon at 375654, 375756. Nonsense mutation around 375654, 375756.", "psc": { "uniref90_id": "UniRef90_UPI0027DFE8A8", "product": "amino acid permease", "uniref50_id": "UniRef50_UPI0027DFE8A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0027DFE8A8", "UniRef:UniRef50_UPI0027DFE8A8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0027DFE8A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8" ], "product": "amino acid permease" } }, "id": "GIHJHMOALN_698", "locus": "GCA009939745_00672" }, { "type": "cds", "sequence": "contig_1", "start": 376038, "stop": 377009, "strand": "+", "frame": 3, "gene": null, "product": "APC family permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB1D5B2", "UniRef:UniRef90_UPI001FB1D5B2" ], "nt": "GTGCTTTTGAGCACCTTAATAGTAGCACTTATAGTTATCGGCAAAAATGGTAATGGTTATGCCATTAACAACGACATAAAACAAGGCCCTTTTAAAGAACGTGAAATTACCTTGGGTGCAGTTGCTACTGTCTTTATTACCTTCTTTTATTCCTACAGTGGTGCGGAGGACATTTCAACAATGACACCGGATGTGAAAACCACTAACTTCCGCAAGATTTTAATCATCTCTTTTATTGCGGTATTCTTGTTTTACTTTGTGGGCATTTTGATCTTTAATGGTTTATTGAATGTTTCACAAAGCGATGATGATAAGGGGAAAGATGGAAGAATAGCTAGTGTAGCTGATATTTTTAGATTAGCTGGCGGTTTAGGGGCACTCATTTTTTACGGTGTGGGTGCTTTATTCAATAACGTATCTACCAGACTTTCGACCATTATTGCTAACTCACGCAAGATCTTACCGTTAGCACAAGATAACTATTTACCCACTACCTTTAGCAAAGCTAACAGTAAGGGCGAGTTCCAAAATGCAATTTGGTTTACCTTTGGTACTACCATCATTACGATGACGGTACTGTTCTTTGTTCCGCTAATAACCAGCAACTTTGACTTCGACAAATCAACCGAGTATGCCGCCTCGATAGGTTCGGCCGCTACACTGGTACAATATATCCTTGTATTTTTTATAATTTTTAGGCTTATTTATAAAAAAGAGCAACTTTATCAAAAAAACTGGACACGCGAGTTAGAACAAATACTGTTTGGGATTGGTGTCTTTATTATTCTTCTCATGTTGCTCTTTTATCTCTTTCCAGTAATTGATGGAAAAACTAAATGGCAATTGAAAAATACCCTCACTTTAGTGGTGTATGGGGTGTTGATTTTAATTGGATTTGCCCTCTTTATGACCCAAGAATATAAGCGCAATAAACAAAAGGAAAGCTATGTCACACCAAGAGCAGTTACATAA", "aa": "MLLSTLIVALIVIGKNGNGYAINNDIKQGPFKEREITLGAVATVFITFFYSYSGAEDISTMTPDVKTTNFRKILIISFIAVFLFYFVGILIFNGLLNVSQSDDDKGKDGRIASVADIFRLAGGLGALIFYGVGALFNNVSTRLSTIIANSRKILPLAQDNYLPTTFSKANSKGEFQNAIWFTFGTTIITMTVLFFVPLITSNFDFDKSTEYAASIGSAATLVQYILVFFIIFRLIYKKEQLYQKNWTRELEQILFGIGVFIILLMLLFYLFPVIDGKTKWQLKNTLTLVVYGVLILIGFALFMTQEYKRNKQKESYVTPRAVT", "aa_hexdigest": "eb16bf6966326977e9ed64777290fb65", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FB1D5B2", "query_cov": 1.0, "subject_cov": 0.8801089918256131, "identity": 0.9940000000000001, "score": 613.0, "evalue": 8.3e-221, "valid": true, "product": "APC family permease", "uniref50_id": "UniRef50_UPI001FB1D5B2" }, "pscc": { "uniref50_id": "UniRef50_UPI001FB1D5B2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB1D5B2" ], "product": "APC family permease" }, "genes": [], "id": "GIHJHMOALN_699", "locus": "GCA009939745_00673" }, { "type": "cds", "sequence": "contig_1", "start": 376984, "stop": 377295, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D8CD42" ], "nt": "ATGTCACACCAAGAGCAGTTACATAAACCTAACCGGCAACAGTTTAGCGAAAAGCAATTTATTGCTTTTGCTTTTAACTATGTTGCTGGGTTCGGCTTTATTTCCGTGGTGCTAACCATGTTTAAGCTTGGCCCGTTTTCTTACTTGATCCTAGGGCTAGCAGCTTTAGGTATTTTGGGGGTAATGTTGTCCTTTTCGCGCCTTTCAATTATTTGTGGGAGCAAGGCTTACGGTGGTAGTTATTTAATAGCCAAAAAAGCACTTGGTGCGAAAACAATCACGGCGCGTTTCTTTACTTTTTTGAGTGGTTGA", "aa": "MSHQEQLHKPNRQQFSEKQFIAFAFNYVAGFGFISVVLTMFKLGPFSYLILGLAALGILGVMLSFSRLSIICGSKAYGGSYLIAKKALGAKTITARFFTFLSG", "aa_hexdigest": "af51c3148a720006783fe73542ca4d54", "start_type": "ATG", "rbs_motif": "AATAA", "psc": { "uniref90_id": "UniRef90_UPI0002D8CD42", "query_cov": 0.9223300970873787, "subject_cov": 0.8878504672897196, "identity": 0.705, "score": 134.0, "evalue": 6.51e-39, "valid": false, "uniref50_id": "UniRef50_UPI0002D8CD42" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D8CD42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D8CD42" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11281.24149999998, "isoelectric_point": 10.08564357757568 }, "id": "GIHJHMOALN_700", "locus": "GCA009939745_00674" }, { "type": "cds", "sequence": "contig_1", "start": 377332, "stop": 377508, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAGCGGTGCTAGTTCTCTCGTTTGCTGATATTAAAGCAGTGAAGGATAACAATGGTGCTTTAATTGGCTTACTGGTCGGGGGCTTTGTTTTATTTGGGGCATTAAACTTCATCTCCCTGTTTGGCCTTAAAATGAATAAGAACGCCATTCTCTTCTTTGCCATAGTGAAGTGA", "aa": "MPAVLVLSFADIKAVKDNNGALIGLLVGGFVLFGALNFISLFGLKMNKNAILFFAIVK", "aa_hexdigest": "ef8547207aa41428e2ef7e35cd19c12f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6155.446099999997, "isoelectric_point": 9.82448215484619 }, "id": "GIHJHMOALN_701", "locus": "GCA009939745_00675" }, { "type": "cds", "sequence": "contig_1", "start": 377533, "stop": 378063, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00324.27" ], "nt": "TTGGGAATCTACCTCATCGGTACGAACCACGGCCATGGTTTTGTCGAAAACAACACTTTAGGTGAACACATTGAAGATCTCAGTTTCCTTAAAGTTATTTCTACTACTGTGGGGATGTTGGTGGCCTTTGCTGGTACCGAAGATCTTACTGCGATTACCCCGGATGTGAAGTCAAAAAACATCCGTAAGTGTTTCTTATTGATGTTTGGTGCAGTTACCCTGCTTTATCTAATCGGTTTTGTCATTATTAGTGGGATTTCTGGTTTAAATGGTTATGGCCTTGATGGTAAAGAAAAGAATGAAAAAGCGATTAACACCTTTGGTTCGATTTACTTTCAAGCAGGCGGTAAGTATTTAGGCATCCCACTGTTGGTCATCTTTGGTCTGGGGTTTTTACTCAATTCCTTGGCATCACGATTAGGCATGACCATTACGACAGCGCGCAAATACGTTGCGTTAGCCCAAGATGGTTTCTTGCCGTCCTTTATTAACGAACAAAACAAGCACCACGAGTACCATAAGGCAGTGTGA", "aa": "MGIYLIGTNHGHGFVENNTLGEHIEDLSFLKVISTTVGMLVAFAGTEDLTAITPDVKSKNIRKCFLLMFGAVTLLYLIGFVIISGISGLNGYGLDGKEKNEKAINTFGSIYFQAGGKYLGIPLLVIFGLGFLLNSLASRLGMTITTARKYVALAQDGFLPSFINEQNKHHEYHKAV", "aa_hexdigest": "d078806c438e1273d34bd674bb283a15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00324.27", "name": "AA_permease", "length": 169, "aa_cov": 0.9431818181818182, "hmm_cov": 0.9349112426035503, "evalue": 1.6783182520579556e-13, "score": 45.527122497558594, "start": 11, "stop": 176 } ], "seq_stats": { "molecular_weight": 19119.021000000008, "isoelectric_point": 7.866964149475098 }, "id": "GIHJHMOALN_702", "locus": "GCA009939745_00676" }, { "type": "cds", "sequence": "contig_1", "start": 378076, "stop": 378291, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTTAGCAGTGATGGTGCTGATGATCATTGTCCCCTTTTTACCTAATGATGAAAACCCCGGTAAACAGCTCGTTATGTTCGATGCCATTAGTGTTTTAGTTGAAGTGGCGATTGAACTAGCAGTGTTAATTTCCTTAATCCAGTACTTTATTACCTACATCTTCTTCTTTATGATTCTGGCCAAAAAAGAGGGTAGTGCCAGTGTTTCGTGA", "aa": "MTLAVMVLMIIVPFLPNDENPGKQLVMFDAISVLVEVAIELAVLISLIQYFITYIFFFMILAKKEGSASVS", "aa_hexdigest": "19ea4c89d06288c76d5d0ab287d188c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7897.521499999995, "isoelectric_point": 4.3588346481323255 }, "id": "GIHJHMOALN_703", "locus": "GCA009939745_00677" }, { "type": "cds", "sequence": "contig_1", "start": 378867, "stop": 379145, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00303.24" ], "nt": "ATGAGCTTTAAAATAACCGTTTTAAGTTATTGCAAAAACTCTTTTAAAAAAGTTATTCGCTTATTTTGCAAGCTTAAAAAAAGATTTTTATACAATGCTACACCATCGAAGTTTAGGAGAGTTATGCAGCAGTATCTAGATTTAGCCCGTTATGTTTTAGAGCACGGAAAATACCGTCCAAATCGCACTGATACTGCTGGCATTGGTGTCTTTGGTTACCAGATGCACTTTGATATTTCAAAACACTTTCCGCTCTTGACTACGAAGAAGGTGCACTGA", "aa": "MSFKITVLSYCKNSFKKVIRLFCKLKKRFLYNATPSKFRRVMQQYLDLARYVLEHGKYRPNRTDTAGIGVFGYQMHFDISKHFPLLTTKKVH", "aa_hexdigest": "8badef1771ba78bade1c445ee768f036", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00303.24", "name": "Thymidylat_synt", "length": 51, "aa_cov": 0.5434782608695652, "hmm_cov": 1.0, "evalue": 3.082204895258385e-15, "score": 51.4938850402832, "start": 43, "stop": 92 } ], "seq_stats": { "molecular_weight": 10995.94419999999, "isoelectric_point": 10.29677753448486 }, "id": "GIHJHMOALN_704", "locus": "GCA009939745_00678" }, { "type": "cds", "sequence": "contig_1", "start": 379482, "stop": 379853, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00303.24" ], "nt": "ATGTTGCTCCCGCCTTGTCATTCACTGTACCAGTTTTACGTGCAGGACGGGCAGTTGAGCTGCCAGTTGTACCAGCGCAGTGGTGATATTTTTCTAGGTATCCCCTTCAACATTGCGTCCTACTCTCTTCTGGTGTACTTAGTTGCTAAGGAAACTAACCTAAAACCAGGGAGCTTTGTCCACACCATTGGCGATGCCCACATTTACGAAAATCACTTGGAACAAATTAAGTTGCAATTAACACGTCAACCCAAACCATTGCCCAAGGTAGTTTTAAAGAGTGATAAATCAATTTTTGATTATCAATTCGATGATATTGAACTGGTGGATTACGACCACCACCCCACAATTAAAGGAGAGGTAGCAGTTTAA", "aa": "MLLPPCHSLYQFYVQDGQLSCQLYQRSGDIFLGIPFNIASYSLLVYLVAKETNLKPGSFVHTIGDAHIYENHLEQIKLQLTRQPKPLPKVVLKSDKSIFDYQFDDIELVDYDHHPTIKGEVAV", "aa_hexdigest": "299a13719ecd353eabe1e055efd5df75", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00260828A0", "query_cov": 1.008130081300813, "subject_cov": 0.8104575163398693, "identity": 0.637, "score": 174.0, "evalue": 8.86e-54, "valid": false, "gene": "thyA", "product": "thymidylate synthase", "ec_ids": [ "2.1.1.45" ], "uniref50_id": "UniRef50_A0A059X7H5", "kegg_orthology_id": "K00560", "go_ids": [ "GO:0004799", "GO:0006231" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00303.24", "name": "Thymidylat_synt", "length": 126, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.317900300773307e-56, "score": 186.31985473632812, "start": 1, "stop": 123 } ], "seq_stats": { "molecular_weight": 14107.032299999983, "isoelectric_point": 5.7831628799438475 }, "id": "GIHJHMOALN_705", "locus": "GCA009939745_00679" }, { "type": "cds", "sequence": "contig_1", "start": 379978, "stop": 380337, "strand": "+", "frame": 1, "gene": "folA", "product": "Dihydrofolate reductase", "db_xrefs": [ "COG:COG0262", "COG:H", "EC:1.5.1.3", "GO:0004146", "GO:0005829", "GO:0006730", "GO:0046452", "GO:0046654", "GO:0046655", "GO:0050661", "KEGG:K00287", "SO:0001217", "UniRef:UniRef50_P78028", "UniRef:UniRef90_P78028" ], "nt": "ATGGGCTCGGCTACCTATCTTTCCTTACCGCCGGTGTTTAGTGAGCGTAACGTTTATATCCTCACCCGTAACCTCAACTTTAACCCGCCTGATAAGGGCTGTTTGACTAAAGTAATCCATGAATATGAAAACTTTATCCAACCTTATTTGCACCATCCCGATAAACACCTCTACATCTGTGGTGGGGCACAAGTTTATGAACAGCTCATTCCCCGGTGTGATGCGTTAATTGTCTCTACCATTTTCGGTAAATACACCGGGGACAAATACCTCAAAGTGGATTTTAGTCCCTTTGAATTGACCAAAGAGATCAGTTTTGCTGAGTTTAAGGTCGCTTACTATCACAAAATTGCACGATAA", "aa": "MGSATYLSLPPVFSERNVYILTRNLNFNPPDKGCLTKVIHEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTIFGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR", "aa_hexdigest": "06c5e173ec0cec6d25ce0aa36513a4ef", "start_type": "ATG", "rbs_motif": null, "genes": [ "folA" ], "truncated": "5-prime", "pseudogene": { "start": 379855, "stop": 380337, "observations": { "mutation": [ 379870, 379918 ], "stop": [ 379870, 379918 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78028", "query_cov": 1.0, "subject_cov": 0.74375, "identity": 1.0, "score": 251.0, "evalue": 3.2e-84, "gene_start": 1, "gene_end": 119, "reference_start": 42, "reference_end": 160, "reference_sequence": "MVKAIWAMDQNGLIGNGNSLPWRIKAELQHFRQTTLHQDVLMGSATYLSLPPVFSERNVYILTRNLNFNPPDKGCLTKVIHEYENFIQPYLHHPDKHLYICGGAQVYEQLIPRCDALIVSTIFGKYTGDKYLKVDFSPFELTKEISFAEFKVAYYHKIAR" }, "paralog": false, "identity": 0.9875, "score": 325.0, "evalue": 1.12e-115, "description": "Internal stop codon at 379870, 379918. Nonsense mutation around 379870, 379918.", "psc": { "uniref90_id": "UniRef90_P78028", "gene": "folA", "product": "Dihydrofolate reductase", "ec_ids": [ "1.5.1.3" ], "uniref50_id": "UniRef50_P78028", "cog_id": "COG0262", "cog_category": "H", "kegg_orthology_id": "K00287", "go_ids": [ "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78028", "EC:1.5.1.3", "UniRef:UniRef50_P78028", "COG:COG0262", "COG:H", "KEGG:K00287", "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ] }, "pscc": { "uniref50_id": "UniRef50_P78028", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78028" ], "product": "Dihydrofolate reductase" } }, "id": "GIHJHMOALN_706", "locus": "GCA009939745_00680" }, { "type": "cds", "sequence": "contig_1", "start": 380344, "stop": 380436, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAACACAAAAAAATACTTTTTAGAATCAGTTTCTCCCTTAGAATATGCACAAAAAAAGTTTCAGGGCAACTTGCGCTCGGTTAACTGA", "aa": "MANTKKYFLESVSPLEYAQKKFQGNLRSVN", "aa_hexdigest": "fa66f01242337ad0ed68da612541c86a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3491.9673000000003, "isoelectric_point": 9.69799518585205 }, "id": "GIHJHMOALN_707", "locus": "GCA009939745_00681" }, { "type": "cds", "sequence": "contig_1", "start": 380692, "stop": 381354, "strand": "+", "frame": 1, "gene": null, "product": "ribonucleoside-diphosphate reductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A376CKW9" ], "nt": "ATGGTGGGGGTACACGCGCGCTCTTATGGCACCATCTTTTCCACTCTGTGTACTTCAGAACAAATTACTGAAGCACATGAATGGGTGGTGAAAACCGAAAGCTTGCAAAAGCGCGCTAAGGCTTTAATTCCCTATTACACCGGGAAAGACCCATTAAAGTCTAAGGTAGCAGCCGCTTTAATGCCCGGGTTCTTACTGTATGGTGGGTTTTATTTACCGTTTTACCTCTCCTCGCGTAAACAGCTACCCAACACTTCGGATATTATTCGCTTAATTTTGCGCGATAAGGTAATCCATAACTATTACAGTGGGTATAAGTTCCAGCGTAAAGTGGAAAAGATGTCCAAGGAAAAACAGGCTGAGATGAAACGGTTTGTCTTTGATTTAATGTACGAACTGATTGAGTTGGAAAAGGCATACCTCAAGGAACTGTATGAAGGGTTTGGGATTGTAGAAGATGCCATTAAATTCAGTATTTATAACGCTGGTAAGTTTTTGCAAAACTTAGGGTATGACTCACCGTTTACAGAAGAAGAAACGCGGATTAAACCAGAAATTTTTGCCCAATTGTCAGCCAGAGCCGATGAAAACCACGATTTCTTTTCTGGTAATGGTTCGTCATATGTGATGGGAATTAGTGAAGAGACCGAAGATAAGGATTGA", "aa": "MVGVHARSYGTIFSTLCTSEQITEAHEWVVKTESLQKRAKALIPYYTGKDPLKSKVAAALMPGFLLYGGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKFQRKVEKMSKEKQAEMKRFVFDLMYELIELEKAYLKELYEGFGIVEDAIKFSIYNAGKFLQNLGYDSPFTEEETRIKPEIFAQLSARADENHDFFSGNGSSYVMGISEETEDKD", "aa_hexdigest": "4567b7e2d05af6d18aceebc0821d9282", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A9E3ENY0", "query_cov": 1.0, "subject_cov": 0.9565217391304348, "identity": 0.505, "score": 227.0, "evalue": 4.71e-72, "valid": false, "gene": "nrdF", "product": "class 1b ribonucleoside-diphosphate reductase subunit beta", "ec_ids": [ "1.17.4.1" ], "uniref50_id": "UniRef50_A0A376CKW9", "go_ids": [ "GO:0004748", "GO:0005971", "GO:0009263" ] }, "pscc": { "uniref50_id": "UniRef50_A0A376CKW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A376CKW9" ], "product": "ribonucleoside-diphosphate reductase" }, "genes": [], "id": "GIHJHMOALN_708", "locus": "GCA009939745_00682" }, { "type": "cds", "sequence": "contig_1", "start": 381366, "stop": 381827, "strand": "+", "frame": 3, "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "db_xrefs": [ "COG:COG1780", "COG:F", "GO:0009263", "GO:0010181", "GO:0036211", "KEGG:K03647", "RefSeq:WP_014325461.1", "SO:0001217", "UniParc:UPI0001DD652A", "UniRef:UniRef100_A0AAV5NAT1", "UniRef:UniRef50_P47472", "UniRef:UniRef90_P75460" ], "nt": "ATGCATAAAGATATCAAAATAGTCGACGCTAGTGCGATCGTTAAACCAACTGGAACACCGTATGTAGTGTATTTTTCCTCCATTTCTAATAACACCCACCGTTTTATTGAAAAATTGGGGTTTGAACACACCCGCATTCCGGTTAATTTAGATGAACAGATTGAAGTAAACCAGGAGTATGTGCTCTTTTGCCCCACTTACAGTGGTGGTGGGGAATACACATCGGGGGCGGTACCCAAACAGGTAATCCACTTTTTAAACAACAAGCACAACCGTGATTTGTGTCGTGGCGTAATTTCGTCCGGGAACACTAATTTCGGCAATACTTTTGCCATTGCCGGGCCGATTTTGTCGAAAAAGTTAAACGTACCACTTTTATACCAGTTTGAACTTTTAGGCACGAAAAACGATGTAGAACAAGTTCAAACCATTATTACCAATTTCTTTGGTAAAGCCAAATAA", "aa": "MHKDIKIVDASAIVKPTGTPYVVYFSSISNNTHRFIEKLGFEHTRIPVNLDEQIEVNQEYVLFCPTYSGGGEYTSGAVPKQVIHFLNNKHNRDLCRGVISSGNTNFGNTFAIAGPILSKKLNVPLLYQFELLGTKNDVEQVQTIITNFFGKAK", "aa_hexdigest": "8416fb583e6ec22566c1c84e88a63f1d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD652A", "ncbi_nrp_id": "WP_014325461.1", "uniref100_id": "UniRef100_A0AAV5NAT1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD652A", "RefSeq:WP_014325461.1", "UniRef:UniRef100_A0AAV5NAT1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NAT1", "uniref90_id": "UniRef90_P75460" }, "psc": { "uniref90_id": "UniRef90_P75460", "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "uniref50_id": "UniRef50_P47472", "cog_id": "COG1780", "cog_category": "F", "kegg_orthology_id": "K03647", "go_ids": [ "GO:0009263", "GO:0010181", "GO:0036211" ] }, "pscc": { "uniref50_id": "UniRef50_P47472", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47472" ], "product": "Protein NrdI" }, "genes": [ "nrdI" ], "id": "GIHJHMOALN_709", "locus": "GCA009939745_00683" }, { "type": "cds", "sequence": "contig_1", "start": 381851, "stop": 383479, "strand": "+", "frame": 2, "gene": null, "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "nt": "ATGTCAGTTAAAGAAAAGATTCCTGCTTTCAACACCCAAGAAGACTTAGAAAGCTATATTTCCTTAAATGCCTACACTAAGGTGTACGGTGATTTTAAGATGGATTTACACGCTGTGGAGGCTTACATCCAAGAGCATGTCAAACCCAAAACAAAGGTGTTTCACTCCACCAAGGAACGCTTAGACTTTCTAGTAAAGAATGATTATTACGATGAGAACATCATCAACATGTACAGCTTTGAGCAGTTTGAGGAAATTACACGTAAGGCTTACGCTTACCGTTTTCGTTATGCTAACTTTATGGGGGCATTTAAGTTTTACAACGCTTATGCACTGAAAACCTTTGATGGGAAGTGGTATTTAGAAAACTATGAAGACCGCGTTGTCATGAACGTGCTCTTTTTAGCCAATGGTAACTATAACAAGGCTTTAAAGTTACTCAAACAAATTATTACCAACCGCTTTCAACCAGCTACCCCAACTTTTTTAAATGCGGGACGAAAAAAACGTGGTGAGTTTGTGTCGTGTTACTTACTGCGCATAGAAGACAACATGGAATCAATTGGTCGTGCCATTACCACTACCCTACAGCTCTCCAAGCGTGATGGTGGGGTGGCGTTATTGCTCACTAACATCCGCGAATCGGGTGCGCCAATTAAAAAAATAGAAAACCAGTCATCAGGCATTATTCCAATTATGAAGCTGTTGGAAGACTCTTTTTCTTACGCCAACCAATTAGGGCAAAGACAGGGAGCAGGAGCAGTGTATCTCCATGCCCACCACCCCGATGTCATGCAGTTTTTAGACACAAAGCGCGAAAACGCTGATGAAAAGATCCGGATTAAGTCGCTGTCTTTGGGTCTAGTTATTCCCGACATTACCTTTACTTTAGCTAAAAACAATGAGGAGATGGCCTTGTTTAGTCCGTATGATGTGTATGAGGAGTATGGCAAACCCTTGTCAGACATTTCAGTCACGGAAATGTATTACGAACTGTTAGCGAACCAACGCATTAAAAAGACCTTTATTAATGCGCGCAAGTTCTTCCAAACAGTGGCGGAACTGCACTTTGAAAGCGGTTATCCTTACATCTTATTTGACGATACGGTTAACCGGCGCAACGCGCACCCCAACCGCATTGTCATGTCTAACCTCTGTAGTGAAATTGTCCAACCCTCCACTCCATCGGAGTTCCACCACGATTTGGCTTTTAAAAAGGTTGGTAATGACATTAGCTGTAACTTGGGTAGCTTAAACATTGCCAAAGCAATGGAAAGCGGACCGGAGTTCTCGGAGTTGGTTAAACTAGCGATCGAATCACTTGATTTAGTTTCAAGGGTAAGCAATTTAGAAACGGCTCCATCGATCCAAAAGGGTAATTCCGAAAATCATGCTTTGGGATTAGGGGCAATGAACTTGCACGGTTTTTTAGCAACTAACCAAATTTATTACAACTCCCCTGAAGCAATCGACTTTACTAACATCTTTTTCTATACGGTAGCTTACCATGCCTTTAAAGCATCCAGTGAGCTAGCTTTAGAGAAGGGTAAGTTTAAAAACTTTGAAAACACCAAGTTTGCTGATGGGAGCTACTTTGATAAATATATCAAAGTAGAACCAGACTTTTGA", "aa": "MSVKEKIPAFNTQEDLESYISLNAYTKVYGDFKMDLHAVEAYIQEHVKPKTKVFHSTKERLDFLVKNDYYDENIINMYSFEQFEEITRKAYAYRFRYANFMGAFKFYNAYALKTFDGKWYLENYEDRVVMNVLFLANGNYNKALKLLKQIITNRFQPATPTFLNAGRKKRGEFVSCYLLRIEDNMESIGRAITTTLQLSKRDGGVALLLTNIRESGAPIKKIENQSSGIIPIMKLLEDSFSYANQLGQRQGAGAVYLHAHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFTLAKNNEEMALFSPYDVYEEYGKPLSDISVTEMYYELLANQRIKKTFINARKFFQTVAELHFESGYPYILFDDTVNRRNAHPNRIVMSNLCSEIVQPSTPSEFHHDLAFKKVGNDISCNLGSLNIAKAMESGPEFSELVKLAIESLDLVSRVSNLETAPSIQKGNSENHALGLGAMNLHGFLATNQIYYNSPEAIDFTNIFFYTVAYHAFKASSELALEKGKFKNFENTKFADGSYFDKYIKVEPDF", "aa_hexdigest": "cd26c88bade2a43f72ffb6d4e5e9a016", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001C049A88", "query_cov": 0.9022140221402214, "subject_cov": 0.9683168316831683, "identity": 0.6809999999999999, "score": 682.0, "evalue": 5.51e-242, "valid": false, "product": "ribonucleoside-diphosphate reductase subunit alpha", "ec_ids": [ "1.17.4.1" ], "uniref50_id": "UniRef50_UPI0021AFF10F", "go_ids": [ "GO:0004748", "GO:0005971", "GO:0009265" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021AFF10F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha" }, "genes": [], "id": "GIHJHMOALN_710", "locus": "GCA009939745_00684" }, { "type": "cds", "sequence": "contig_1", "start": 383585, "stop": 384016, "strand": "+", "frame": 2, "gene": null, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "nt": "TTGGCCAATTCCCACTTACTGGCAATTGCTCCAACGGGTTCAATTTCCTATCTCTCCTCCTGTACTCCTTCACTCCAACCGGTAGTATCCCCGGTAGAAGTGCGCAAGGAAGGTCGGTTGGGACGGATTTATGTACCCGCTTACCAACTCAATAAAGACAGTTACCCTTTTTATAAAGATGGTGCTTACGAACTGGGTCCCGAACCAATTATTAACATAGCAGCAGCGGCACAACAACACGTCGATCAAGCGATTTCGCTCACCCTATTTATGACTGATAAGGCTACCACTCGTGACCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTTCTTCCATTTACTATGTCCGAGTGCGCCAGGAAGTACTAGAGGACAGTGAGGATCACACCATTCAAATGCAGCAGTGTGAGGCCTGTGTGATTTAA", "aa": "MANSHLLAIAPTGSISYLSSCTPSLQPVVSPVEVRKEGRLGRIYVPAYQLNKDSYPFYKDGAYELGPEPIINIAAAAQQHVDQAISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQEVLEDSEDHTIQMQQCEACVI", "aa_hexdigest": "fb6271ea63a8efc0623df6849ecd1699", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A655JDN6", "query_cov": 0.986013986013986, "subject_cov": 0.9929577464788732, "identity": 0.518, "score": 147.0, "evalue": 4.24e-43, "valid": false, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "uniref50_id": "UniRef50_A0A655JDN6" }, "pscc": { "uniref50_id": "UniRef50_A0A655JDN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "product": "Ribonucleotide-diphosphate reductase subunit alpha" }, "genes": [], "id": "GIHJHMOALN_711", "locus": "GCA009939745_00685" }, { "type": "cds", "sequence": "contig_1", "start": 384163, "stop": 384465, "strand": "+", "frame": 1, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02888", "RefSeq:WP_010874681.1", "SO:0001217", "UniParc:UPI0000133D48", "UniRef:UniRef100_P78026", "UniRef:UniRef50_P47474", "UniRef:UniRef90_P78026" ], "nt": "ATGCATGCTATTGTAGTCTGTGGATCAAAGCAATATTTAGTCCACGAAAATGACACTTTTTTTGTTGAAAAACTTGAAGCGCCAGTTGGTAAAGAAATCCAACTCGACAAGGTTTTAATGTTGGATGAAAAGATTGGTGCGCCATACTTGGAAAAAGCGCGAGTAGTGTGTGTGGTGGAAAAGCACGGTTTACAACGCAAAGTAAACGTGATTAAACACATCTCACAAAAACACCACTTAAAAAAGTATGGTCACCGTCAACCTTACACTAAGTTAAAGGTAGTGCGCTTTGTCCATGATTAA", "aa": "MHAIVVCGSKQYLVHENDTFFVEKLEAPVGKEIQLDKVLMLDEKIGAPYLEKARVVCVVEKHGLQRKVNVIKHISQKHHLKKYGHRQPYTKLKVVRFVHD", "aa_hexdigest": "f4fd694f151cc62406782937221bdfff", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D48", "ncbi_nrp_id": "WP_010874681.1", "uniref100_id": "UniRef100_P78026", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D48", "RefSeq:WP_010874681.1", "UniRef:UniRef100_P78026" ] }, "ips": { "uniref100_id": "UniRef100_P78026", "uniref90_id": "UniRef90_P78026" }, "psc": { "uniref90_id": "UniRef90_P78026", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_P47474", "cog_id": "COG0261", "cog_category": "J", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47474", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47474" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "GIHJHMOALN_712", "locus": "GCA009939745_00686" }, { "type": "cds", "sequence": "contig_1", "start": 384458, "stop": 384760, "strand": "+", "frame": 2, "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "db_xrefs": [ "COG:COG2868", "COG:S", "EC:3.4.22.-", "GO:0006508", "GO:0008234", "GO:0042254", "KEGG:K07584", "RefSeq:WP_010874682.1", "SO:0001217", "UniParc:UPI00001394CB", "UniRef:UniRef100_P75459", "UniRef:UniRef50_P75459", "UniRef:UniRef90_P75459" ], "nt": "ATGATTAAACTAACTGTGTCGCACCACAAACTCACCGCTTCCGGGCACGCTTTATTTGCCAAAAAGGGGCAAGACATTGTGTGCGCTGCTGTTAGTGGTATTATCTTTGGCGCTTTGCCCTGGTTTGAAACCAACAGCATTGCCGTTCAAGAAGATGCTACTGTACCCAGTTTAAGCTTGGAGTTAGTGCAACCAACCGCTAAGCTTATCACAGGATTGAGTGTGGTGATTATGCAACTTAAAACCCTCGCGCATTCTTACCCGCAGTTTATTTCGTTTGAAGATCAACGTAAAGATGAATAA", "aa": "MIKLTVSHHKLTASGHALFAKKGQDIVCAAVSGIIFGALPWFETNSIAVQEDATVPSLSLELVQPTAKLITGLSVVIMQLKTLAHSYPQFISFEDQRKDE", "aa_hexdigest": "74427003d8133d4a4fdf9c3971842635", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394CB", "ncbi_nrp_id": "WP_010874682.1", "uniref100_id": "UniRef100_P75459", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394CB", "RefSeq:WP_010874682.1", "UniRef:UniRef100_P75459" ] }, "ips": { "uniref100_id": "UniRef100_P75459", "uniref90_id": "UniRef90_P75459" }, "psc": { "uniref90_id": "UniRef90_P75459", "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "ec_ids": [ "3.4.22.-" ], "uniref50_id": "UniRef50_P75459", "cog_id": "COG2868", "cog_category": "S", "kegg_orthology_id": "K07584", "go_ids": [ "GO:0008234", "GO:0006508", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75459", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75459" ], "product": "Ribosomal processing cysteine protease Prp" }, "genes": [ "prp" ], "id": "GIHJHMOALN_713", "locus": "GCA009939745_00687" }, { "type": "cds", "sequence": "contig_1", "start": 384753, "stop": 385067, "strand": "+", "frame": 3, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "SO:0001217", "UniParc:UPI0001DD652D", "UniRef:UniRef100_A0AAX0SQQ5", "UniRef:UniRef50_P05657", "UniRef:UniRef90_P75458" ], "nt": "ATGAATAACAAATATTTTTTAACCAAAATTGACTTACAGTTTTTTGCTTCCAAAAAGGGAGTTGGTTCAACCAAGAACGGCCGTGATTCTCATGCTAAGCGTTTAGGTGCTAAGAAGGCTGATGGTCAGATGATCCGTACTGGTCAAATTATTTACCGCCAACGCGGTACTAGGGTATGTCCCGGTGTAAATGTTGGCCTTGGTAGTGATGACACCTTGTTTGCTTTAAGCGACGGCTTGGTAAAATACCAAAAGTTTGGACCTAAACAGGGCAAGACCCGCGTGAGTGTGGTCAAACACAAACTAGATGCCTAA", "aa": "MNNKYFLTKIDLQFFASKKGVGSTKNGRDSHAKRLGAKKADGQMIRTGQIIYRQRGTRVCPGVNVGLGSDDTLFALSDGLVKYQKFGPKQGKTRVSVVKHKLDA", "aa_hexdigest": "a847ff3e5cabef129e95365463dcb5c7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD652D", "uniref100_id": "UniRef100_A0AAX0SQQ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD652D", "UniRef:UniRef100_A0AAX0SQQ5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQQ5", "uniref90_id": "UniRef90_P75458" }, "psc": { "uniref90_id": "UniRef90_P75458", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_P05657", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P05657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05657" ], "product": "Large ribosomal subunit protein bL27" }, "genes": [ "rpmA" ], "id": "GIHJHMOALN_714", "locus": "GCA009939745_00688" }, { "type": "cds", "sequence": "contig_1", "start": 385060, "stop": 385905, "strand": "+", "frame": 1, "gene": "nfo", "product": "putative endonuclease 4", "db_xrefs": [ "COG:COG0648", "COG:L", "EC:3.1.21.2", "GO:0003677", "GO:0003906", "GO:0006284", "GO:0008081", "GO:0008270", "GO:0008833", "SO:0001217", "UniRef:UniRef50_P47477", "UniRef:UniRef90_P75457" ], "nt": "ATGCCTAAACTCCTAGGAAGCTTTATTTCGTTTAAATCACCCCATTATCTAGTGGGGAGTGTTCGTGACGCTGTTAGCATTAAAGCACAAGCGTTCATGATTTTTTTGGGCGCACCACACACGGCACTACGAGTCGATCCTAATCGTATGCAAATTGACGAGGGTCACACATTGATGGAACAACATAACCTCAGTAAGTCTGGGATGGTTGTCCATGCTCCTTACATTATTAACTGTGCCTCCAAAGATCCGGTAAAGCAAACCTTTGCGATTGACGTTCTGACAAGGGAGGTTAAACTGTGTCATGCCGTGGGTGCTAAACTAATCGTTCTCCACCCCGGTTCAGCGGTTGAGCAAACTCAAACCCAAGCGTTAGATCACCTAATCAAGGTCTTAAATACAGTGATTGCCAACACTAAAGAAGTCATTATTTGTCTCGAAACGATGGCTGGTAAAGGTAATGAAATTGGCCGTGATTTAGACCAGTTAAAGTATGTGATTAACCACATTGAACAACAAGAGCGGATTGGTGTTTGTCTAGATACCTGTCACTTCCATGACAGTGGTAATGACTTTAACAATACTGCCGAAATTATGGAAACGATTGACACTAAACTAGGCTTTGAGTTTTTAAAGGTCATCCACTTAAACGAGTCCAAAAACGTTTGTGGTTCCAAAAAAGACCGTCATGCCAATCTAGGTGAGGGGATGATTGGGTTTGATAATTTAATGCGCTTTATTGCCCAACCGCAAATAAAGCAAATACCGATTGTTTTAGAAACCCCATCTGACAAGCATAATTATCCCGCTGTATATGGTGCTGAAATCGAAAGGATCCGTGCTTGA", "aa": "MPKLLGSFISFKSPHYLVGSVRDAVSIKAQAFMIFLGAPHTALRVDPNRMQIDEGHTLMEQHNLSKSGMVVHAPYIINCASKDPVKQTFAIDVLTREVKLCHAVGAKLIVLHPGSAVEQTQTQALDHLIKVLNTVIANTKEVIICLETMAGKGNEIGRDLDQLKYVINHIEQQERIGVCLDTCHFHDSGNDFNNTAEIMETIDTKLGFEFLKVIHLNESKNVCGSKKDRHANLGEGMIGFDNLMRFIAQPQIKQIPIVLETPSDKHNYPAVYGAEIERIRA", "aa_hexdigest": "62a774368a256e0f18ae38b14afee857", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75457", "query_cov": 1.0, "subject_cov": 0.9825174825174825, "identity": 1.0, "score": 566.0, "evalue": 6e-204, "valid": true, "gene": "nfo", "product": "Probable endonuclease 4", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_P47477", "cog_id": "COG0648", "cog_category": "L", "go_ids": [ "GO:0008833", "GO:0003677", "GO:0003906", "GO:0008081", "GO:0008270", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_P47477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47477" ], "product": "Probable endonuclease 4" }, "genes": [ "nfo" ], "id": "GIHJHMOALN_715", "locus": "GCA009939745_00689" }, { "type": "cds", "sequence": "contig_1", "start": 385874, "stop": 386350, "strand": "+", "frame": 2, "gene": "fur", "product": "putative MG236-like protein", "db_xrefs": [ "COG:COG0735", "COG:P", "GO:0000976", "GO:0003700", "GO:0008270", "GO:0045892", "GO:1900376", "RefSeq:WP_010874685.1", "SO:0001217", "UniParc:UPI00001394D1", "UniRef:UniRef100_P75456", "UniRef:UniRef50_P75456", "UniRef:UniRef90_P75456" ], "nt": "ATGGTGCTGAAATCGAAAGGATCCGTGCTTGATTTGGAGCACGATAACCAAACACTAAAGGATTACATTAGTGTGTTTGAACGCAATAAGATGCGTTTAACCCAATCACGCTTAATGTTGCTCCAATGCTTAGTGCAACACCGTGACTGGCACACCTTAGCTGAGCTCAAACACCACTTGGAACAAAACCAACAGCGCACTACCTTAGCTTCTATCTATAACAACCTCAAGATTTTTGCTAAGTTAAAGCTAATTAATATTTTTGTAGATACCAATCGCTTTGAAACGTATTATTGCTTAAGACACGAAAACCACAAGCACATTTACTTCTTTGACGAAAACCAGCGCAAGTTTTTAACCCTACCACTACAAGACAAGGAAGCGTTGAGTTTAATTGGACATAAAAGTAAACATGGCAAAATTAAACTGAATGACTTTTACATAGTCGCTAGCGGTACACTTGAAGATGATCAATAA", "aa": "MVLKSKGSVLDLEHDNQTLKDYISVFERNKMRLTQSRLMLLQCLVQHRDWHTLAELKHHLEQNQQRTTLASIYNNLKIFAKLKLINIFVDTNRFETYYCLRHENHKHIYFFDENQRKFLTLPLQDKEALSLIGHKSKHGKIKLNDFYIVASGTLEDDQ", "aa_hexdigest": "6ae7dda45d01c26158f5d460a185332a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D1", "ncbi_nrp_id": "WP_010874685.1", "uniref100_id": "UniRef100_P75456", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D1", "RefSeq:WP_010874685.1", "UniRef:UniRef100_P75456" ] }, "ips": { "uniref100_id": "UniRef100_P75456", "uniref90_id": "UniRef90_P75456" }, "psc": { "uniref90_id": "UniRef90_P75456", "gene": "Fur", "product": "Uncharacterized protein MG236 homolog", "uniref50_id": "UniRef50_P75456", "cog_id": "COG0735", "cog_category": "P", "go_ids": [ "GO:0003700", "GO:0000976", "GO:0008270", "GO:0045892", "GO:1900376" ] }, "pscc": { "uniref50_id": "UniRef50_P75456", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75456" ], "product": "Uncharacterized protein MG236 homolog" }, "genes": [ "fur" ], "id": "GIHJHMOALN_716", "locus": "GCA009939745_00690" }, { "type": "cds", "sequence": "contig_1", "start": 386340, "stop": 387224, "strand": "+", "frame": 3, "gene": null, "product": "putative MG237-like protein", "db_xrefs": [ "RefSeq:WP_010874686.1", "SO:0001217", "UniParc:UPI00001394D4", "UniRef:UniRef100_P75455", "UniRef:UniRef50_P75455", "UniRef:UniRef90_P75455" ], "nt": "ATGATCAATAAGCCAAATCAGTTTGTCAACCACTTAAGTGCTTTAAAAAAACACTTTGCCTCTTATAAGGAGCTCCGTGAAGCGTTTAACGATTATCACAAGCATAACGGTGATGAGTTGACCACCTTCTTTTTGCACCAGTTTGATAAGGTGATGGAACTAGTCAAGCAGAAGGACTTTAAAACTGCCCAGTCTCGCTGTGAGGAGGAATTAGCCGCCCCTTATCTACCAAAACCTTTAGTAAGCTTTTTCCAATCGCTACTCCAGCTCGTGAACCATGATTTATTGGAACAGCAAAACGCGGCCTTGGCTAGTTTACCCGCAGCCAAAATCATAGAGCTTGTTTTACAGGATTACCCCAATAAACTCAACATGATTCACTACCTTTTGCCCAAAACGAAGGCGTTTGTCAAACCACATTTGTTACAACGCTTACAGTTTGTGTTAACTGATTCGGAGTTGTTGGAATTAAAGCGCTTTTCCTTCTTTCAAGCACTCAACCAGATCCCGGGCTTTCAGGGTGAGCAAGTTGAGTACTTTAACAGTAAGCTCAAACAAAAGTTTACCTTGACCTTAGGGGAGTTTGAAATTGCCCAACAGCCAGATGCCAAAGCATACTTTGAGCAGTTAATTACCCAAATCCAACAGCTCTTTTTAAAAGAGCCGGTTAACGCTGAGTTTGCCAATGAGATTATTGATGCCTTTTTGGTGAGCTATTTCCCCCTCCATCCCCCAGTACCTTTGGCACAATTAGCTGCCAAGATTTATGAGTATGTCAGCCAAATTGTGCTTAACGAAGCGGTTAACCTAAAGGATGAACTGATCAAACTAATCGTTCATACGCTTTACGAACAGTTAGATCGTCCTGTTGGGGATGAAAATTAA", "aa": "MINKPNQFVNHLSALKKHFASYKELREAFNDYHKHNGDELTTFFLHQFDKVMELVKQKDFKTAQSRCEEELAAPYLPKPLVSFFQSLLQLVNHDLLEQQNAALASLPAAKIIELVLQDYPNKLNMIHYLLPKTKAFVKPHLLQRLQFVLTDSELLELKRFSFFQALNQIPGFQGEQVEYFNSKLKQKFTLTLGEFEIAQQPDAKAYFEQLITQIQQLFLKEPVNAEFANEIIDAFLVSYFPLHPPVPLAQLAAKIYEYVSQIVLNEAVNLKDELIKLIVHTLYEQLDRPVGDEN", "aa_hexdigest": "e70a6b3f75a97b33ae1981c5cba6968a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D4", "ncbi_nrp_id": "WP_010874686.1", "uniref100_id": "UniRef100_P75455", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D4", "RefSeq:WP_010874686.1", "UniRef:UniRef100_P75455" ] }, "ips": { "uniref100_id": "UniRef100_P75455", "uniref90_id": "UniRef90_P75455" }, "psc": { "uniref90_id": "UniRef90_P75455", "product": "Uncharacterized protein MG237 homolog", "uniref50_id": "UniRef50_P75455" }, "pscc": { "uniref50_id": "UniRef50_P75455", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75455" ], "product": "Uncharacterized protein MG237 homolog" }, "genes": [], "id": "GIHJHMOALN_717", "locus": "GCA009939745_00691" }, { "type": "cds", "sequence": "contig_1", "start": 387372, "stop": 388583, "strand": "+", "frame": 3, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "COG:COG0544", "COG:O", "EC:5.2.1.8", "GO:0003755", "GO:0005737", "GO:0005854", "GO:0006457", "GO:0015031", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0140839", "GO:0140840", "SO:0001217", "UniRef:UniRef50_P47480", "UniRef:UniRef90_P75454" ], "nt": "ATGAAAATCAAGGGTTTTCGTAAAGGTAAAGTGCCTCCAACTTTAGCTAAGGATTACTTAGACCGTGCCGAGTTGTTGCAACGTAGCGCCCAAGCGGTAATTGATGCCATCTTCCAGCCGTTACAACAAGAAGCAGTAATTGCTGACAACGAGAATGTAATTGAGGACTTTCCTACCATTGACTTTAAAACAATCAATGAGAACGATTGTGTTATTCTGTTTGACTTTGATCTCGTACCCCAGTTTGAACTACCTGATTACAAGCACATCAAGGATTTAAGCCCGATTGTACCCCTAAAGGATGAGGAGTTCAACAAGGAGTTGCACAACATCGAAAAGAACAAAGGGAAACTAGTTGATGTTAGTGACAAAGCATTGGCCAACAATGATATTGCCGTCATTGACTTTGTTGGCAAGGTAGATGGTAAGGTGCTTGAAAGCGCTACTGCCAAACAATACGAATTAACGATTGGCTCCAACAGCTTTATTGATGGCTTTGAAAGTGGTTTAATTGGCATGAAAGTAGGGGACAAGCGTCAGTTAAAGTTAAAGTTCCCCAAGGACTACCATGCTGAAGAATTGAAGGGTAAACCAGTAGAGTTTGACATAGAGCTCAAAGCAATTAAGCAGTTAGAGATCACCCCCATGGATGAAACTAACTTTAAGGAGTATTTACCCGCACAATACCAGGGTTTCAACTCACTCAAGGAATTTAAAACTTACTTTCATAAGTTAGTTTCCGCTAAGAAACTGGAAATTACCCTACAAGAAAACAGTGTTAAAATTCGCCAGTTCTTTTTAGCTAACACCACCTTACCTTACATTCCTGATTCCTTAATTAAATTGGAATCCGATCGCTTACTGAGAGCGCAAAAGGATCAAGCGGAGCAGTACAAGATTCCCTTTGAGAGATTGTTAGCAGCTTCCAAGCTTTCATTGGAGCAGTTGCAACAACGTAACATAAAAGAAGCACGTGATAACGTTACCTTTGCTTTAGTGATGAAACGAATCGCTGATGTGGAAAAAATCAAGGTTGATAACAAAAAGATTCACAGCGAAATCGAATCGATTATTGATGTTGAATACCCCTTTGTTAATGCCGAGTTGAAAAAACAAATGTTCCACAACATGGAACAACAAAAGGACTTTGTGGAATCGATAATTTTGAACCGTTTAACCACTACCAAGATAATTGAGTACTCCACTCATTAG", "aa": "MKIKGFRKGKVPPTLAKDYLDRAELLQRSAQAVIDAIFQPLQQEAVIADNENVIEDFPTIDFKTINENDCVILFDFDLVPQFELPDYKHIKDLSPIVPLKDEEFNKELHNIEKNKGKLVDVSDKALANNDIAVIDFVGKVDGKVLESATAKQYELTIGSNSFIDGFESGLIGMKVGDKRQLKLKFPKDYHAEELKGKPVEFDIELKAIKQLEITPMDETNFKEYLPAQYQGFNSLKEFKTYFHKLVSAKKLEITLQENSVKIRQFFLANTTLPYIPDSLIKLESDRLLRAQKDQAEQYKIPFERLLAASKLSLEQLQQRNIKEARDNVTFALVMKRIADVEKIKVDNKKIHSEIESIIDVEYPFVNAELKKQMFHNMEQQKDFVESIILNRLTTTKIIEYSTH", "aa_hexdigest": "70eea71f7649d9473dd157ddb7dfcae4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75454", "query_cov": 1.0049627791563276, "subject_cov": 0.9080717488789237, "identity": 0.995, "score": 765.0, "evalue": 5.22e-278, "valid": true, "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_P47480", "cog_id": "COG0544", "cog_category": "O", "go_ids": [ "GO:0003755", "GO:0005737", "GO:0005854", "GO:0006457", "GO:0015031", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0140839", "GO:0140840" ] }, "pscc": { "uniref50_id": "UniRef50_P47480", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47480" ], "product": "Trigger factor" }, "genes": [ "tig" ], "id": "GIHJHMOALN_718", "locus": "GCA009939745_00692" }, { "type": "cds", "sequence": "contig_1", "start": 388654, "stop": 389634, "strand": "+", "frame": 1, "gene": null, "product": "LON peptidase substrate-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E", "UniRef:UniRef90_UPI001F21836E" ], "nt": "ATGCCAGCTGTAAAAAAACCACAAATTCTCGTTGTGCGTAACCAAGTTATCTTTCCGTACAACGGCTTTGAACTTGATGTCGGTCGTGAACGCTCGAAAAAACTAATTAAAGCACTCAAAAACCTCAAAACCAAACGTTTGGTGTTAGTCACCCAAAAGAATTCAGATCAGCTCAACCCTGAGTTTGATGACATTTACCATTGCGGTACTTTATGTGACATTGATGAAATTATTGAAGTACCGAGTGAAGATGGCAAGACAGCTGACTATAAGATTAAGGGTAAAGGATTACAGCGCGTTGCCATTACAAGCTTCTCTGATGCTGATTTAACCAAGTACGACCATCACTTTCTCAACTCTACCCTAACAGAAAATAAAGCGTTAGATAAGTTGTTGGAACGGATCTTTCCCGATAAAGAAGACTTTGCTGAAATTTTAGACAGCTTAAACAGCTTCTTGGAACTGCAAGAGTTAAAGAAGCTATCTAAGGTACCAAAAGACATAAAACGGTATGACATTATTACCTTCAAGCTTGCTAGCTTAATTTTCAAAGACATTACCCTCCAACAAGCCATTTTGGAAGAAAATGACATTGAAAAACGGTTACAGAAGATCATTGGTTCTGGCATCGAGGATTTAGGTCACATCAGCGAAGAAGCCCGCGCTAAACAACGCGAAAGTGAGTTTGACAAGATTGACAACCGCATCACGCGCAAAGTTAACGAACAGTTATCGCGCCAACAACGTGACTTTTACCTCCGGGAAAAACTCCGGGTTATCCGTGAAGAGATCGGGATGACTTCCAAGAAGGAAGATGAAGTATCCAACATTCGCAAAAAGCTAGAGGAAAACCCATATCCAGAGCACATTAAAAAACGGATTTTAAGTGAGTTAGATCACTTTGAAAACTCCTCCTCTTCATCACAAGAATCAACCTTAACCAAGACTTACATCGACACTTTGATGAACTTGCCATGGTGA", "aa": "MPAVKKPQILVVRNQVIFPYNGFELDVGRERSKKLIKALKNLKTKRLVLVTQKNSDQLNPEFDDIYHCGTLCDIDEIIEVPSEDGKTADYKIKGKGLQRVAITSFSDADLTKYDHHFLNSTLTENKALDKLLERIFPDKEDFAEILDSLNSFLELQELKKLSKVPKDIKRYDIITFKLASLIFKDITLQQAILEENDIEKRLQKIIGSGIEDLGHISEEARAKQRESEFDKIDNRITRKVNEQLSRQQRDFYLREKLRVIREEIGMTSKKEDEVSNIRKKLEENPYPEHIKKRILSELDHFENSSSSSQESTLTKTYIDTLMNLPW", "aa_hexdigest": "f5dded132c55a2ffa45747c08e59f906", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F21836E", "query_cov": 0.8006134969325154, "subject_cov": 1.0, "identity": 0.985, "score": 490.0, "evalue": 1.52e-173, "valid": true, "product": "LON peptidase substrate-binding domain-containing protein", "uniref50_id": "UniRef50_UPI001F21836E" }, "pscc": { "uniref50_id": "UniRef50_UPI001F21836E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E" ], "product": "LON peptidase substrate-binding domain-containing protein" }, "genes": [], "id": "GIHJHMOALN_719", "locus": "GCA009939745_00693" }, { "type": "cds", "sequence": "contig_1", "start": 389680, "stop": 391041, "strand": "+", "frame": 1, "gene": "lon", "product": "endopeptidase La", "db_xrefs": [ "COG:COG0466", "COG:O", "EC:3.4.21.53", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565", "SO:0001217", "UniRef:UniRef50_P47481", "UniRef:UniRef90_P78025" ], "nt": "TTGAACAAGAACCACTCAGGACTAGATAAGGTAAAGGAACGAGTGGTTGAGTATTTAGCAGTACAGCTCCGTACCAAAAAGCTTAAGGGTCCGATTATGTGTTTAGTCGGTCCACCTGGAGTTGGTAAATCCAGTTTAGCCAAATCAATTGCTGAAGCGTTAAACAAGCGCTTTGTTAAGGTTTCATTGGGCGGGGTGCATGATGAATCGGAAATCCGTGGTCACCGTAAAACCTATTTGGGTTCGATGCCAGGACGGATTTTAAAGGGCATGGCACGTGCTAAGGTTATTAACCCACTGTTCTTGTTAGATGAAATTGACAAGATGACATCCTCCAATCAAGGTTACCCTTCCGGCGCTTTGTTAGAGGTATTAGATCCGGAGTTAAACAATAAGTTTAGCGATAACTATGTTGAGGAGGATTATGACCTGTCTAAGGTAATGTTTGTAGCCACCGCTAACTATATCGAAGATATTCCGGAAGCCTTGTTAGACCGGATGGAAGTGATTGAACTTACCTCTTACACCGAACAGGAAAAGCTACAAATTACAAAGAGCCACTTAGTTAAGCGTTGTTTAGATGACGCTGAGATTAAAACAGATGACCTCAAGTTTACCGATGAGGGTATTAGCTATATCATTAAGTTTTACACGAGGGAAGCGGGGGTGAGACAGTTAGAACGCTTAATCCAGCAAATTGTGCGTAAGTACATTGTTAACTTGCAAAAAACCGGCGAACAACAGGTAGTAGTTGATGTAGATTTGGTAAAGAAATACCTCAAGAAAGAAATCTTTGACTATACCGTGCGCGATGAAGATGCACTTCCGGGAATTGTTAACGGCATGGCTTACACCCCAACTGGTGGTGATCTGTTACCAATTGAAGTAACCCATGTAGCTGGTAAGGGTGATTTAATTCTGACCGGCAATTTAAAGCAAACAATGCGTGAAAGTGCTAGCGTAGCGTTAGGTTATGTCAAGGCCAATGCGCAGAGCTTTAACATTAACCCTAACCTCTTTAAAAAGGTTGACATTAACATCCATGTTCCAGGTGGTGGTATTCCCAAGGATGGCCCGAGTGCTGGCGCGGCTTTAGTTACTGCCATTATCTCGTCTTTAACTGGCAAAAAGGTTGACCCTAAGATTGCCATGACCGGGGAGATTACCCTAAGGGGTAAAGTAATGACCATAGGCGGGGTGAAAGAAAAAACCATTTCCGCCTACCGCGGTGGAGTACGCACCATCTTTATGCCCGAAAAGAATGAGCGTTACTTAGATGAAGTGCCTAAAGACATCGTTAAGGACTTGGAGATTATCCTAGTAAAGGAATACAAGGACATCTACAACAAGATATTTAATTAA", "aa": "MNKNHSGLDKVKERVVEYLAVQLRTKKLKGPIMCLVGPPGVGKSSLAKSIAEALNKRFVKVSLGGVHDESEIRGHRKTYLGSMPGRILKGMARAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFVATANYIEDIPEALLDRMEVIELTSYTEQEKLQITKSHLVKRCLDDAEIKTDDLKFTDEGISYIIKFYTREAGVRQLERLIQQIVRKYIVNLQKTGEQQVVVDVDLVKKYLKKEIFDYTVRDEDALPGIVNGMAYTPTGGDLLPIEVTHVAGKGDLILTGNLKQTMRESASVALGYVKANAQSFNINPNLFKKVDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPKIAMTGEITLRGKVMTIGGVKEKTISAYRGGVRTIFMPEKNERYLDEVPKDIVKDLEIILVKEYKDIYNKIFN", "aa_hexdigest": "c5b33583c7d31917b30b13905c76d26b", "start_type": "TTG", "rbs_motif": "AAAAA", "psc": { "uniref90_id": "UniRef90_UPI0025901479", "query_cov": 0.9867549668874173, "subject_cov": 0.8799212598425197, "identity": 0.523, "score": 493.0, "evalue": 7.23e-169, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A7C6Y0Z4", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "genes": [ "lon" ], "truncated": "5-prime", "pseudogene": { "start": 389380, "stop": 391041, "observations": { "mutation": [ 389632 ], "stop": [ 389632 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78025", "query_cov": 1.0, "subject_cov": 0.5490909090909091, "identity": 0.998, "score": 879.0, "evalue": 2.53e-316, "gene_start": 1, "gene_end": 453, "reference_start": 373, "reference_end": 825, "reference_sequence": "MSTPLISTQSIRVLKNVLQFSILQPLILITMPAVKKPQILVVRNQVIFPYNGFELDVGRERSKKLIKALKNLKTKRLVLVTQKNSDQLNPEFDDIYHCGTLCDIDEIIEVPSEDGKTADYKIKGKGLQRVAITSFSDADLTKYDHHFLNSTLTENKALDKLLERIFPDKEDFAEILDSLNSFLELQELKKLSKVPKDIKRYDIITFKLASLIFKDITLQQAILEENDIEKRLQKIIGSGIEDLGHISEEARAKQRESEFDKIDNRITRKVNEQLSRQQRDFYLREKLRVIREEIGMTSKKEDEVSNIRKKLEENPYPEHIKKRILSELDHFENSSSSSQESTLTKTYIDTLMNLPWWQESKDNADVKNLIKILNKNHSGLDKVKERVVEYLAVQLRTKKLKGPIMCLVGPPGVGKSSLAKSIAEALNKRFVKVSLGGVHDESEIRGHRKTYLGSMPGRILKGMARAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFVATANYIEDIPEALLDRMEVIELTSYTEQEKLQITKSHLVKRCLDDAEIKTDDLKFTDEGISYIIKFYTREAGVRQLERLIQQIVRKYIVNLQKTGEQQVVVDVDLVKKYLKKEIFDYTVRDEDALPGIVNGMAYTPTGGDLLPIEVTHVAGKGDLILTGNLKQTMRESASVALGYVKANAQSFNINPNLFKKVDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPKIAMTGEITLRGKVMTIGGVKEKTISAYRGGVRTIFMPEKNERYLDEVPKDIVKDLEIILVKEYKDIYNKIFN" }, "paralog": false, "identity": 0.9981916817359855, "score": 1078.0, "evalue": 0.0, "description": "Internal stop codon at 389632. Nonsense mutation around 389632.", "psc": { "uniref90_id": "UniRef90_P78025", "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_P47481", "cog_id": "COG0466", "cog_category": "O", "go_ids": [ "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78025", "EC:3.4.21.53", "UniRef:UniRef50_P47481", "COG:COG0466", "COG:O", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ] }, "pscc": { "uniref50_id": "UniRef50_P47481", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47481" ], "product": "Lon protease" } }, "id": "GIHJHMOALN_720", "locus": "GCA009939745_00694" }, { "type": "cds", "sequence": "contig_1", "start": 391353, "stop": 391892, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12679.13" ], "nt": "GTGACACTGTTAGCGGGCAGTATTACCGCTGACATCTTTATTGAAGGGTACAACAACGGGATTGAGCTGTTCTTGTTCTCTAAACCTATTAGCCGTGACCGCATTTTGCTCATTAAGTTACTAGTGCTGTTAATGTACTTGAGTATTGTAGCCGTACTATCCACTGCTGTAGTGAGCTTGGGTTACTTTACGCGCAATGAGATTGAGAGTTATTTAGATGATTTAGCTTTAAGTATCTTTGTCGGTACCATTTTAAACGGCATTATCTTTAGCGCTGTAACTATTATGTTAGCCACGCGCTTTAGCAACATCCAGAGCTTAATCATTTTGCTCTTGTTTGTGTCGTTGTTTAGCTTTTCCTCACCAATTGCGCGGTTAATCTTCGACACGCCTGCTAAAATGTTGAGCCAACAAGGTTACAGCGTTCGCCAAATTAACGGAATTGCGCACAGCGAAAGCCCTAATGTAGAGCGTAAAATTTACGTTTCGGTCAAAAAGCGGCGTTTAACTGGGGTGGATCGCGAAGAAAAAGAGCTTTGA", "aa": "MTLLAGSITADIFIEGYNNGIELFLFSKPISRDRILLIKLLVLLMYLSIVAVLSTAVVSLGYFTRNEIESYLDDLALSIFVGTILNGIIFSAVTIMLATRFSNIQSLIILLLFVSLFSFSSPIARLIFDTPAKMLSQQGYSVRQINGIAHSESPNVERKIYVSVKKRRLTGVDREEKEL", "aa_hexdigest": "20febea09cbc846dfa573b01ed52a144", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12679.13", "name": "ABC2_membrane_2", "length": 128, "aa_cov": 0.7039106145251397, "hmm_cov": 0.9921875, "evalue": 1.2854775576427303e-09, "score": 33.192134857177734, "start": 3, "stop": 128 } ], "seq_stats": { "molecular_weight": 19998.346099999995, "isoelectric_point": 8.92314968109131 }, "id": "GIHJHMOALN_721", "locus": "GCA009939745_00695" }, { "type": "cds", "sequence": "contig_1", "start": 391929, "stop": 392336, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGAGGAGGCACAACGAACCTCCTCTTACATTAATTCCTTTTACACCAACATTGGTTACTTATTTGGCTCGCTTTACCGCTTGGGTGCGTTAGCTGATGAAAATAACTTTGATGCATCAGTAGCAACCCAAAACACCAAAATTCGCTTTGGTAAGTTAGTTGATTTAGATAAGAGTACTGACATTATTGGTTATCGCACTAAAGACCGCTTTGGCCGTTTCTTTCGCTTTCACTTGCGCTTTCCAACGCTCAACTTTAAGCAAAGCTTGAGTAACAGCGGCGTTTTCTTTATCAATAAACGGGGATTAGTTAAAGCTACTTTTAGTGCTGGCGATGATGCCTTTAAACGTGACCCGAAGATAATGCAAAAGGTGTTTGACACTTTTCTAGAAAGTTTTAGTTGA", "aa": "MYEEAQRTSSYINSFYTNIGYLFGSLYRLGALADENNFDASVATQNTKIRFGKLVDLDKSTDIIGYRTKDRFGRFFRFHLRFPTLNFKQSLSNSGVFFINKRGLVKATFSAGDDAFKRDPKIMQKVFDTFLESFS", "aa_hexdigest": "ecd16d1d22dc9eb0d09bd42a00d2966c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15598.45779999998, "isoelectric_point": 9.652222633361816 }, "id": "GIHJHMOALN_722", "locus": "GCA009939745_00696" }, { "type": "cds", "sequence": "contig_1", "start": 392526, "stop": 392633, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAGTGAAAGCACTGCCACCGGTATTTCTAGTGATCCCCAAAGTCAGGGCAAAATGAACAACAAAAGTGAGGAACAAAAAAAGAAAGAAAAAGAACTCACTTGA", "aa": "MSSESTATGISSDPQSQGKMNNKSEEQKKKEKELT", "aa_hexdigest": "0601b64c4b95ea4f51e647d93046f2b4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3844.1550000000007, "isoelectric_point": 6.117035102844238 }, "id": "GIHJHMOALN_723", "locus": "GCA009939745_00697" }, { "type": "cds", "sequence": "contig_1", "start": 392697, "stop": 393488, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGGGACAATGTAGCTTTTTTAAATAAAGTGAACGCTGAAGACCAAAACGGGGATAAAAACCAGTTTGCCCAAGCAATGGCATCACTCAATAAGCAAATTAACATGTTTTACGTGTTGAATGAAATTACCAACAAGGAACACAATTTGCCAACTAAATACGGTGCTTATTCTGATCAATTAAAAAAGGTCATAAACGAAAACTTTGAAAAGATAGAAAATAAGAAAAAGGAGATAGAGGAAAAACAAAATAAAGAATTTCAGGAAAACAAGAATGGTGCATCAAATAATCAAGACACAAAGCAAGACGCACAAAAAGGTGATACCAATACCCAAAGCACTGAGTTAAAAGCTCGTCAAGCACAACAAGTGCAAAAGGATCAACAAAATGACAGTAAAGGCAATACTGCTACAAACAGTGATACTGGTAAATCGGACAATAAAACCGATACCACTGAAGATGAAAAAAACACATATAAACCCTTAACAGCTAGGGAGAAAAAAACCAAAATTAAGGAGCTTACTAAGGACATCTTCTTCTCTACAGCGCAGAACTATTTGTTCTTAGTGCAAGGTGGGTATAACAACAAGTTGTTTGCTGGTGGTTTACCAGAGCTCCACCAACGCTTCTTGTCAGACGTAGTGGTTGATTTCTCTGAGGAAAAACTCGTTTATGAAGTACAAGGTGAACCGATTGTATCCAGTGTAGCCACCATTGTGATTACCCTATTTTTAACAGTTGTTTTATTAGCTATTGCCTTTTTCTTCTTTAAATACCGCAACGTTAAATAG", "aa": "MWDNVAFLNKVNAEDQNGDKNQFAQAMASLNKQINMFYVLNEITNKEHNLPTKYGAYSDQLKKVINENFEKIENKKKEIEEKQNKEFQENKNGASNNQDTKQDAQKGDTNTQSTELKARQAQQVQKDQQNDSKGNTATNSDTGKSDNKTDTTEDEKNTYKPLTAREKKTKIKELTKDIFFSTAQNYLFLVQGGYNNKLFAGGLPELHQRFLSDVVVDFSEEKLVYEVQGEPIVSSVATIVITLFLTVVLLAIAFFFFKYRNVK", "aa_hexdigest": "fc672e2a964a620b8511d19cc8977dbd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30068.203000000023, "isoelectric_point": 6.2375337600708 }, "id": "GIHJHMOALN_724", "locus": "GCA009939745_00698" }, { "type": "cds", "sequence": "contig_1", "start": 393587, "stop": 393856, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCCCGGTGAGTTCCATGCCTTTATTGGTCAAAATGGTGCGGGGAAAACTACCACCATTAAGTGTTTAATTTCATCTTATCAGCGCTTTAAGGACGAAATTAACATCGATGGCATTAGCAATAAAAACGCCAAGTCTAAGGGTGTAATTAGTTATATCCCTGAATATGCTGTGTTTCCCAAACACCTCAATACCCATGAATACCTCTACACGTTGGGTAAACTGTCAGGTTGTAGTTTGCAAACCATTAAGGAAAAGGTTGATTACTGA", "aa": "MPGEFHAFIGQNGAGKTTTIKCLISSYQRFKDEINIDGISNKNAKSKGVISYIPEYAVFPKHLNTHEYLYTLGKLSGCSLQTIKEKVDY", "aa_hexdigest": "e43cb07d3092f0b783e035908a936b46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 69, "aa_cov": 0.7415730337078652, "hmm_cov": 1.0, "evalue": 2.441288145212061e-11, "score": 39.8638801574707, "start": 2, "stop": 67 } ], "seq_stats": { "molecular_weight": 9961.279999999993, "isoelectric_point": 8.887047386169435 }, "id": "GIHJHMOALN_725", "locus": "GCA009939745_00699" }, { "type": "cds", "sequence": "contig_1", "start": 393974, "stop": 394228, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAGCCCACTGCTAACTTAGATCCCAAAACACGTAATGAGTTTATGGATGTGTGTTACGAACTTAATGTACGTAACAAGATGGCGGTGTTTGTTTCATCCCATATTTTGGCTGAATTGGAAAACTATTGTGATTCGCTCACTGTTATTCATGAAGGGCAAATTCTTTTTAATGGCAAAACAAAGAACATTGCCAAAGACAACTTTGGTTACCGTTTAAAGGTTAACCATTCCGATAGTTTACGTAAGTGA", "aa": "MDEPTANLDPKTRNEFMDVCYELNVRNKMAVFVSSHILAELENYCDSLTVIHEGQILFNGKTKNIAKDNFGYRLKVNHSDSLRK", "aa_hexdigest": "1d12abd41efc0d6efdb48e53de64b691", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9691.906899999987, "isoelectric_point": 6.261519813537597 }, "id": "GIHJHMOALN_726", "locus": "GCA009939745_00700" }, { "type": "cds", "sequence": "contig_1", "start": 394674, "stop": 395315, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912123E", "UniRef:UniRef90_UPI001912123E" ], "nt": "ATGTCGAGTTTAATCATTCCTTTCATTGCTCTGTTTGCCACCATTATTACCTTTCAAGTCTTTATTAATGGTTATTACAACGGTCTAGAGATCTTATTAATTACCCGCTTTTTTACTCGCGGTCGGTTATTCTTTGCTCGCTTAGCAGTCTTGTTTATTTGGATTACTGGCACGGCATTTTTAAGTGGTCTTTTTGTTAGTTTAACTGCTACTTTAGGTGCTACATCACAAACAGTTACTGACCTTGTTTTAAGCGTCTTTTTTGGAGTGCTTTTACTTAGTTTATTGTTTAGCTGTGTCTTGATTATTATCGCGCAGTTTTTAAACCGCACCCAAAGTATGTTGTTGTTACTGTTAGGGGTTTCCCTAGCTAGCTTTACAACCGTTATCTTGGCCTTTTCAATTAAGCCACCGAGTGAAAATCTCCGTGATAATAGTTACCAACCACTAAACCTAAGTCTCATCAGTAAGTCTAAGTCCAAGGGTGTCGAAAATGTCTTTACCATCATTGACTCTTCAAATGTATCCGATCCGCGCAATAAAAAGAAATCGATTGTAAAAACCAATCCGCAAGCGATTTGGGATGAATATGGGGAGAGTAGCTTCTTTCAAAGCCAGTATTACTGGAATGTAGGGTATTGA", "aa": "MSSLIIPFIALFATIITFQVFINGYYNGLEILLITRFFTRGRLFFARLAVLFIWITGTAFLSGLFVSLTATLGATSQTVTDLVLSVFFGVLLLSLLFSCVLIIIAQFLNRTQSMLLLLLGVSLASFTTVILAFSIKPPSENLRDNSYQPLNLSLISKSKSKGVENVFTIIDSSNVSDPRNKKKSIVKTNPQAIWDEYGESSFFQSQYYWNVGY", "aa_hexdigest": "1552749d2db6258031c2ea01960eba80", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 394458, "stop": 395618, "observations": { "mutation": [ 394476, 394557, 394566, 394653, 395313 ], "stop": [ 394476, 394557, 394566, 394653, 395313 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001912123E", "query_cov": 1.0, "subject_cov": 0.49882903981264637, "identity": 1.0, "score": 384.0, "evalue": 3.89e-131, "gene_start": 1, "gene_end": 213, "reference_start": 73, "reference_end": 285, "reference_sequence": "MKLFKNWSDVNNVKQRQLLLAYFRFILKQTVKSWLLWVTAGLVLFLLALVLLIIPAFTKQDPLFLWSHPVVQMSSLIIPFIALFATIITFQVFINGYYNGLEILLITRFFTRGRLFFARLAVLFIWITGTAFLSGLFVSLTATLGATSQTVTDLVLSVFFGVLLLSLLFSCVLIIIAQFLNRTQSMLLLLLGVSLASFTTVILAFSIKPPSENLRDNSYQPLNLSLISKSKSKGVENVFTIIDSSNVSDPRNKKKSIVKTNPQAIWDEYGESSFFQSQYYWNVGYWINSLFRLNSLSDYRNFDVNLYFLNTKLNFDREVDTDKMVDFVSFRDQRHLYFLSYSFFINTHNLPTQPLPRILTYDNNGAFLEKIVPSFDDIKLDPPRVKKVYKFFSDTIIQSFRDYESEEKERQEKEEKEKAEKDNGNGQ" }, "paralog": false, "identity": 0.9870466321243523, "score": 734.0, "evalue": 1.52e-271, "description": "Internal stop codon at 394476, 394557, 394566, 394653, 395313. Nonsense mutation around 394476, 394557, 394566, 394653, 395313.", "psc": { "uniref90_id": "UniRef90_UPI001912123E", "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI001912123E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001912123E", "UniRef:UniRef50_UPI001912123E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001912123E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912123E" ], "product": "ABC transporter permease" } }, "id": "GIHJHMOALN_727", "locus": "GCA009939745_00701" }, { "type": "cds", "sequence": "contig_1", "start": 395427, "stop": 396683, "strand": "+", "frame": 3, "gene": "nosY", "product": "putative protein MPN_335", "db_xrefs": [ "COG:COG1277", "COG:O", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75443", "UniRef:UniRef90_P75443" ], "nt": "ATGGTCGACTTTGTTAGCTTTCGCGATCAAAGACACCTTTACTTTTTAAGTTATTCCTTTTTTATCAATACCCATAACCTGCCCACACAACCGTTACCCCGGATTCTTACTTATGACAATAACGGTGCCTTTTTAGAGAAAATAGTCCCTTCCTTTGATGACATAAAACTAGATCCACCCCGGGTCAAAAAGGTTTACAAATTCTTTAGTGACACCATTATCCAGTCCTTCCGTGATTATGAAGCGGAGGAAAAAGAAAGGCAGGAAAAGGAAGAGAAAGAAAAGGCTGAAAAGGACAATGGCAACGGCCAGGATTCCAATAAAGTTAACTCTGTCAGCACGGAGCCTAACAATAAGAATTCATCAGATGCGGATTCTAAGGATAACAATGATTCATCCGATTCCCAAGGCAAAGACTCAAGTAAGAGTAAGCCTAAGTTTAGACCTAGGCTGCCACAATTCTTTGATCGTGTTTCCATTAACTACAGTAAGTTTTCCAGTAGCTTTAACACACAACTAAAACACATTCATCAAGGACTTTCTACCCGTGATGAGGCCATGGAAATCTTTAAAGATAAGGTAGCGTTATTTTATGCCCTATCAATGGCTTATGACAACTTTACTTTCCAAAAGGACAGTGGCATTATTGATAACACTAGCTTAAACAAATTTAAAACAGAGTTTAACAAGAAGCAAAAAGAGCTAACTGAAAAAAACAAGCAAAAACAGGACGGTAAAGACCAAAAGAGTCAAAGAATGACACAAGGTGCTGATAAAGCAGTCACTGTTGAGCCAAAAGCTATGCAGTCTGAAATTGGAATGACTGATCAAACTAATGACCAAAGCAGTTCGGAAACAAAGGACAGCATGGATAGTTCAGACAGTAGTGATACTGTTGATAACACTGATGAATCAGAAGATAAACAAAGTGAAGAGGAGGAAAAGTTCGACGAGGAAATTGAGAATGCCAAAAAAATGCCTAAGGCTGAAGACGCCTTTTTTAATACTGCCTCTATCTGGTTGAGCTCACCATTTCTCTTTTTTGAAAATGGTGCAAAACGCGAACAACGATACGAAATCCATTTGCTCAATTCAAACACACAGGTTAATAACGAAATAATTAAAACTAACAGCTTTTATTACGTTAGTGGTGAACCGATTGTAGGCCAAGAAGTCATTATTGCAATGGTGCTGGTGGTAACACTAGGTCTGTTAGTTGGTAGTTTCTTCGCTTACCAAAAACGCGATATTAAGTAA", "aa": "MVDFVSFRDQRHLYFLSYSFFINTHNLPTQPLPRILTYDNNGAFLEKIVPSFDDIKLDPPRVKKVYKFFSDTIIQSFRDYEAEEKERQEKEEKEKAEKDNGNGQDSNKVNSVSTEPNNKNSSDADSKDNNDSSDSQGKDSSKSKPKFRPRLPQFFDRVSINYSKFSSSFNTQLKHIHQGLSTRDEAMEIFKDKVALFYALSMAYDNFTFQKDSGIIDNTSLNKFKTEFNKKQKELTEKNKQKQDGKDQKSQRMTQGADKAVTVEPKAMQSEIGMTDQTNDQSSSETKDSMDSSDSSDTVDNTDESEDKQSEEEEKFDEEIENAKKMPKAEDAFFNTASIWLSSPFLFFENGAKREQRYEIHLLNSNTQVNNEIIKTNSFYYVSGEPIVGQEVIIAMVLVVTLGLLVGSFFAYQKRDIK", "aa_hexdigest": "4804eeb1070233fb5a8adc7548ab2efd", "start_type": "ATG", "rbs_motif": null, "genes": [ "nosY" ], "truncated": "5-prime", "pseudogene": { "start": 395127, "stop": 396683, "observations": { "mutation": [ 395313 ], "stop": [ 395313 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75443", "query_cov": 1.0, "subject_cov": 0.5580774365821095, "identity": 1.0, "score": 592.0, "evalue": 9.55e-205, "gene_start": 1, "gene_end": 418, "reference_start": 332, "reference_end": 749, "reference_sequence": "MLLLSVNKMKLFKNWSDVNNVKQRQLLLAYFRFILKQTVKSWLLWVTAGLVLFLLALVLLIIPAFTKQDPLFLWSHPVVQMSSLIIPFIALFATIITFQVFINGYYNGLEILLITRFFTRGRLFFARLAVLFIWITGTAFLSGLFVSLTATLGATSQTVTDLVLSVFFGVLLLSLLFSCVLIIIAQFLNRTQSMLLLLLGVSLASFTTVILAFSIKPPSENLRDNSYQPLNLSLISKSKSKGVENVFTIIDSSNVSDPRNKKKSIVKTNPQAIWDEYGESSFFQSQYYWNVGYWINSLFRLNSLSDYRNFDVNLYFLNTKLNFDREVDTDKMVDFVSFRDQRHLYFLSYSFFINTHNLPTQPLPRILTYDNNGAFLEKIVPSFDDIKLDPPRVKKVYKFFSDTIIQSFRDYEAEEKERQEKEEKEKAEKDNGNGQDSNKVNSVSTEPNNKNSSDADSKDNNDSSDSQGKDSSKSKPKFRPRLPQFFDRVSINYSKFSSSFNTQLKHIHQGLSTRDEAMEIFKDKVALFYALSMAYDNFTFQKDSGIIDNTSLNKFKTEFNKKQKELTEKNKQKQDGKDQKSQRMTQGADKAVTVEPKAMQSEIGMTDQTNDQSSSETKDSMDSSDSSDTVDNTDESEDKQSEEEEKFDEEIENAKKMPKAEDAFFNTASIWLSSPFLFFENGAKREQRYEIHLLNSNTQVNNEIIKTNSFYYVSGEPIVGQEVIIAMVLVVTLGLLVGSFFAYQKRDIK" }, "paralog": false, "identity": 0.9980694980694981, "score": 795.0, "evalue": 3.5e-287, "description": "Internal stop codon at 395313. Nonsense mutation around 395313.", "psc": { "uniref90_id": "UniRef90_P75443", "gene": "NosY", "product": "Uncharacterized protein MPN_335", "uniref50_id": "UniRef50_P75443", "cog_id": "COG1277", "cog_category": "O", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75443", "UniRef:UniRef50_P75443", "COG:COG1277", "COG:O", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75443", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75443" ], "product": "Uncharacterized protein MPN_335" } }, "id": "GIHJHMOALN_728", "locus": "GCA009939745_00702" }, { "type": "cds", "sequence": "contig_1", "start": 396966, "stop": 397313, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTACAACAGTTTAACGTCCGACTGTTGGGTAAGTGTCCTTTAGATTTGGCATCGAGTGAAATGTTTGGTAGTCATAAATTTCGACAAATTCCCGCTAAGGTGTTGCACTATATCCACCAACACAACATTTATCTAAAAACCATTTTGCAAACACTGTTAGATGAACCGCGCATGCAACACTGTTTGCGCGTTGGTCAGTTAGCTAAAACTTTAGCAGTGGCAAATAAACTCGATGGTAAAACGGCTTATACTGCTGGTGCTTACCATGATCTAGCAAAGCAACTACCCCAAGCACAATTAGAGAAATTAGCCAAAGTAGCGGGTGTAAATGATTACCCTAGCTGA", "aa": "MVQQFNVRLLGKCPLDLASSEMFGSHKFRQIPAKVLHYIHQHNIYLKTILQTLLDEPRMQHCLRVGQLAKTLAVANKLDGKTAYTAGAYHDLAKQLPQAQLEKLAKVAGVNDYPS", "aa_hexdigest": "b0ff642501f2cd9c8351a8382548fdd1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12909.93339999998, "isoelectric_point": 9.48163928985596 }, "id": "GIHJHMOALN_729", "locus": "GCA009939745_00703" }, { "type": "cds", "sequence": "contig_1", "start": 397389, "stop": 397718, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01467.32" ], "nt": "GTGATCTTTGGTGGTGCATTTGATCCCCTCCACCAAGCGCATATCTACATTGCTAAGCGTGCTGTACAAGCCATTAAAGCACAAAAGCTTTACTTTGTACCAACTGCTAAAGCCTTTTTTAAATCACCGATTAAAGCATCTAATCAAGCACGTTTAGCAATGTTAAGGGTTGCGCTAAAAGCATTGCCTCAGATGGCAGTATCTAACTTTGACATTAAAGCGCAAAACGGTTTTTCGTTCAATACAGTACAACACTTTAAGCAGCGCTTTCCCAATGCTGAGTTGTACTTTTTAATTGGCAGTGATAAATTAAGCGAGTTGGCCAAATGA", "aa": "MIFGGAFDPLHQAHIYIAKRAVQAIKAQKLYFVPTAKAFFKSPIKASNQARLAMLRVALKALPQMAVSNFDIKAQNGFSFNTVQHFKQRFPNAELYFLIGSDKLSELAK", "aa_hexdigest": "63fda029442a289b48af7ffea810f352", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01467.32", "name": "CTP_transf_like", "length": 106, "aa_cov": 0.963302752293578, "hmm_cov": 0.8773584905660378, "evalue": 2.1450187817845986e-12, "score": 42.86591720581055, "start": 2, "stop": 106 } ], "seq_stats": { "molecular_weight": 12231.238799999988, "isoelectric_point": 10.246040916442869 }, "id": "GIHJHMOALN_730", "locus": "GCA009939745_00704" }, { "type": "cds", "sequence": "contig_1", "start": 397803, "stop": 398264, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAAAAAGGAACTAGAAATTTACAACCTCTATACTTTTCAAATAGACCTCGATAAAAAGCTTTTATTTGAAAAAGCAGATAATCAACAGAATTACTCTAAGATTAGAGCTCGTTATTTTAAAAATTCTGCACGTAATCAACAAGCAGTTTTTCTCAATAAAAACCTGATTAAAAACACTTTTAATAAGGCACTGTTAAACTTCTCGGATTTTTTGAGTGGTAGTGGTGTAGAGAGCATTTTTAAGCAGGTCATTGATGATCAACCTGAAGTTTTAAACTACCTCAAACAGGTCAAAAAGGAAGATAGTTGCGATGGTCACAGCGAAGCGTCACAGTTAGTATTTAATGTAGTCATTAACCCTAAAAATACGCTCGCTAACTTCTTTGAGGAATTAACCATTTACCTCCACTTTAATGAAGAGAACAGCACAGTTGTCGGGAGCTTTTCGTTAAAGTGA", "aa": "MVKKELEIYNLYTFQIDLDKKLLFEKADNQQNYSKIRARYFKNSARNQQAVFLNKNLIKNTFNKALLNFSDFLSGSGVESIFKQVIDDQPEVLNYLKQVKKEDSCDGHSEASQLVFNVVINPKNTLANFFEELTIYLHFNEENSTVVGSFSLK", "aa_hexdigest": "74d4e5d709dccd41c377983b0a39602a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17761.914500000003, "isoelectric_point": 6.737716865539551 }, "id": "GIHJHMOALN_731", "locus": "GCA009939745_00705" }, { "type": "cds", "sequence": "contig_1", "start": 398460, "stop": 398612, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGCAAAAGTACCCTTTTTCCACGATTCACAAAAACTTAAAGATTGTCAATTCTGATTTCTTTGATGCTTTTTTTGTGATCTTGTTGTTAATTTGTCACCTCAATAATAACTTGTTGTGGTTGTGTGAAAAAACTGAGCACTTTGAATGA", "aa": "MEQKYPFSTIHKNLKIVNSDFFDAFFVILLLICHLNNNLLWLCEKTEHFE", "aa_hexdigest": "6526f5c90980bb80098122c6ab14354d", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6058.011799999996, "isoelectric_point": 5.81379909515381 }, "id": "GIHJHMOALN_732", "locus": "GCA009939745_00706" }, { "type": "cds", "sequence": "contig_1", "start": 398673, "stop": 399158, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTTGTTTTTAAAGGACTGGTACTTTGAAAACCAAGCCGTTACTAATGAAGACATTGAAAAGGTTGATGAAGTGGAAGACATTGGTAAGTTAGTGGAAAAATACAGCGCTAATCAACCACAAAAGTTGTCTTCTAATTCAACCGTTTATGTATTTGAACCAGACAAAAAACAGTGCTTTTTAAAGAATGATGACTTTTTCAACACTAACGAAGCTAAGCTGTTGTTTTTAATCACAATGCAACCCAATGTTTTTGGTCTGGATGATACTGCCATTGCCAATGACTTAAACCTAAGGGAAATTGGTGACTTCTTTAAGGAAATTGACTTTACTGACAGCGATGTGCTCAATGACTTTCAACAACAAAAAGAAACTTTGTTAGTACGGCGTACCTTTAACCAACTGTTGTTTATGAACAGTAATACCGATGTTTTGAGTATTGTTAACAATAAGTTCAAATCAGCGATCCATAACATTGTCTGA", "aa": "MLLFLKDWYFENQAVTNEDIEKVDEVEDIGKLVEKYSANQPQKLSSNSTVYVFEPDKKQCFLKNDDFFNTNEAKLLFLITMQPNVFGLDDTAIANDLNLREIGDFFKEIDFTDSDVLNDFQQQKETLLVRRTFNQLLFMNSNTDVLSIVNNKFKSAIHNIV", "aa_hexdigest": "1b14b4b571ea121106574fc9cd30a994", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18764.926, "isoelectric_point": 4.459667015075683 }, "id": "GIHJHMOALN_733", "locus": "GCA009939745_00707" }, { "type": "cds", "sequence": "contig_1", "start": 399183, "stop": 399500, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCAAGGCATTTGATTACTCCAAGATCTTTGAGCAAAACCGCACTAACGATCCTTCTCTCTTGCGTTCTAACCTCAACAGCATTAACCGGCTGCGGTATCTCAGTGAATACTTCCGTACCGCTTCAGTTAAGTATGACCAGCTGTACACCAAGGTAAAGGAGTACATGCAATTAGATCAGTTCTTAGTAGACATGATTAACCAGGTCAACCATGAAGATGAGATCTTTGGTAAATACAAGGAACGGGTTTATTTGAGTTTGGGAATAGTTACTGCTGTTGTCTTTGGTATTATTGAGTTCTTTAACTGTGTTTGA", "aa": "MLKAFDYSKIFEQNRTNDPSLLRSNLNSINRLRYLSEYFRTASVKYDQLYTKVKEYMQLDQFLVDMINQVNHEDEIFGKYKERVYLSLGIVTAVVFGIIEFFNCV", "aa_hexdigest": "c5cde1f6fda7d44f9e08dadb56cfb401", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12541.246299999995, "isoelectric_point": 6.566063117980956 }, "id": "GIHJHMOALN_734", "locus": "GCA009939745_00708" }, { "type": "cds", "sequence": "contig_1", "start": 399671, "stop": 400651, "strand": "+", "frame": 2, "gene": null, "product": "MPN338 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47484", "UniRef:UniRef90_P75440" ], "nt": "ATGGAATTCAATAAGTTACAACTCAGTCACTGTATTTCCTTTTACATTTCAGACGTTTCGGAAGTGTTCTTTGAAAGTATTAACCAACACCCTAGTCGTGATTTTGTCAACAACATCCTGCAAAAGATTAAGAGCACTCTCAGTGAAGAGGAATTGGAAAAGCTCAACACCATTGAGGAAGTTACTAAGGATGAGAAAATTTTGATCATGCTCAACCATGTGTTGAAGAAGATTGTTTCTAAAACGGGTAGTTCTAGCTGTGACTTATTTCAAATAGTGAAACACGACCGCTTTAACGAGCCGGTTTACATCCAAAGTGTCAATGCCTTTGAAAACAATTTGATTAACAATGAATTTGCGGAAAGGCGGTATGACTATTTAATTGAAATTAATAAACACTCTTATTTAAGGAAGTATGTCAATGCCATTCGCATCTCCTTCTTTTTAGATTTAAGGGCGCAAATCTTGTCCGGTTCCTTTACTTTGGATGTGATTAACAAACAGTTGGAACACCAAAATAAGGAAGAGTTGTTCCAAGCGATTTACTTACGCTCCTTAATTAAGCATTTTATCTCGAACCAACCTTACCCGATTTCCTTAAATTCGTTTATTTTTGGTGATAAAAACCGTGAAAACAAGACGGTTGAAAACGACAAGCTTAGTGTGCTTAAAAACAATTGGAATCAAATCTTCTTTTCTAAGTACTTTGATTTTGTTGCCAAAAACAAAGAAGAGCGTGTTGTCGATAACAACTGCGATGAGCTGTTTTACGCCACCATGAACACGCTCTTGATTATGTTGATCATTATTGAAGAGCTCCGGGTATACTTCAACAGTAAAGAGCCAGCCTTAATCTTAAAGCTACTAGATAACAAGGTTTCACTGCGCGAAGACCCCGACCAAAATCCAGAAACTGATTTGCATGAGTTAATTAAGTTTGCTGAAAAGAATTACCTCGAAAAGGAAAAAACAAGTCGCTGA", "aa": "MEFNKLQLSHCISFYISDVSEVFFESINQHPSRDFVNNILQKIKSTLSEEELEKLNTIEEVTKDEKILIMLNHVLKKIVSKTGSSSCDLFQIVKHDRFNEPVYIQSVNAFENNLINNEFAERRYDYLIEINKHSYLRKYVNAIRISFFLDLRAQILSGSFTLDVINKQLEHQNKEELFQAIYLRSLIKHFISNQPYPISLNSFIFGDKNRENKTVENDKLSVLKNNWNQIFFSKYFDFVAKNKEERVVDNNCDELFYATMNTLLIMLIIIEELRVYFNSKEPALILKLLDNKVSLREDPDQNPETDLHELIKFAEKNYLEKEKTSR", "aa_hexdigest": "40dcca5b103ddb45eb80196061ac079e", "start_type": "ATG", "rbs_motif": "AACAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 399671, "stop": 400954, "observations": { "mutation": [ 400649 ], "stop": [ 400649 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75440", "query_cov": 1.0, "subject_cov": 0.5158227848101266, "identity": 0.997, "score": 628.0, "evalue": 2.84e-222, "gene_start": 1, "gene_end": 326, "reference_start": 1, "reference_end": 326, "reference_sequence": "MEFNKLQLSHCISFYISDVSEVFFESINQHPSRDFVNNILQKIKSTLSEEELEKLNTIEEVTKDEKILIMLNHVLKKIVSKTGSSSCDLFQIVKHDRFNEPVYIQSVNAFENNLINNEFAERRYDYLIEINKHSYLRKYVNAIRISFFLDLRAQILSGSFTLDVINKQLEHQNKEELFQAIYLRSLIKHFISNQLYPISLNSFIFGDKNRENKTVENDKLSVLKNNWNQIFFSKYFDFVAKNKEERVVDNNCDELFYATMNTLLIMLIIIEELRVYFNSKEPALILKLLDNKVSLREDPDQNPETDLHELIKFAEKNYLEKEKTSRWHKKRVKSLEELLEEIKQINLETKNESLAYPDEIVELELDNVHNFVSTKQVFRHQLDLQTLHGIVINPERYGIGMWSNHFVDWEEFKDLIEQITDAENGSDLYGFEKDLDESICQVNKKYLTFISSDSSSFLIIKNDQTKVISNYVWAQLYFETRRWIINDIEYDLYEKGFDKSHFASNIALLESLNFNWLDPFYGLTSIKEIMQKIDSKSNLKTSIAEMVAKFKHEQRISKKDNERVLMIFAYVAAAVVGFINFFSMVFTILTVSDLNAGLTPANIVVIAIASLLALFLIVIAVLFRFRWKYIKH" }, "paralog": false, "identity": 0.9953161592505855, "score": 842.0, "evalue": 6.25e-309, "description": "Internal stop codon at 400649. Nonsense mutation around 400649.", "psc": { "uniref90_id": "UniRef90_P75440", "product": "MPN338 family protein", "uniref50_id": "UniRef50_P47484", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75440", "UniRef:UniRef50_P47484", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47484" ], "product": "Uncharacterized protein MG242" } }, "id": "GIHJHMOALN_735", "locus": "GCA009939745_00709" }, { "type": "cds", "sequence": "contig_1", "start": 400751, "stop": 401218, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAATTAGAGTTAGATAACGTTCACAACTTTGTCTCTACTAAACAGGTTTTTCGTCACCAGTTAGACTTACAAACTTTACATGGTATAGTCATCAACCCTGAGCGCTATGGTATTGGCATGTGGAGTAACCACTTTGTCGATTGGGAGGAGTTTAAAGATTTAATTGAACAGATTACTGATGCTGAAAATGGTAGTGATCTCTATGGTTTTGAAAAGGACTTGGATGAGAGCATTTGCCAAGTTAACAAGAAGTACTTAACCTTTATTAGTAGTGACAGTAGCTCTTTCTTAATTATTAAGAATGACCAAACTAAGGTTATCTCTAATTATGTCTGGGCGCAACTGTACTTTGAAACGCGCCGGTGGATTATTAATGATATTGAATACGATCTGTATGAAAAGGGGTTTGACAAGAGCCACTTTGCTAGTAACATTGCCCTTTTAGAGAGCTTGAATTTTAACTGA", "aa": "MELELDNVHNFVSTKQVFRHQLDLQTLHGIVINPERYGIGMWSNHFVDWEEFKDLIEQITDAENGSDLYGFEKDLDESICQVNKKYLTFISSDSSSFLIIKNDQTKVISNYVWAQLYFETRRWIINDIEYDLYEKGFDKSHFASNIALLESLNFN", "aa_hexdigest": "6f14ab6eb370bebc7a0ac39820e35106", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18331.2391, "isoelectric_point": 4.609608268737793 }, "id": "GIHJHMOALN_736", "locus": "GCA009939745_00710" }, { "type": "cds", "sequence": "contig_1", "start": 401258, "stop": 401551, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAGATTGACAGTAAGAGTAATTTAAAAACATCGATTGCCGAAATGGTCGCTAAGTTTAAACACGAACAGCGCATCTCCAAAAAGGACAATGAGCGGGTGTTGATGATCTTTGCTTATGTAGCCGCTGCGGTAGTTGGTTTTATTAACTTTTTTTCGATGGTGTTTACCATTCTTACAGTCAGTGATCTCAATGCTGGTCTCACCCCCGCTAACATTGTTGTTATTGCAATTGCGAGCTTATTGGCCTTATTTTTAATTGTTATTGCTGTTTTATTTCGGTTTAGGTGA", "aa": "MQKIDSKSNLKTSIAEMVAKFKHEQRISKKDNERVLMIFAYVAAAVVGFINFFSMVFTILTVSDLNAGLTPANIVVIAIASLLALFLIVIAVLFRFR", "aa_hexdigest": "939c945166e706743f54903491bbe67b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10793.860399999985, "isoelectric_point": 9.991777610778808 }, "id": "GIHJHMOALN_737", "locus": "GCA009939745_00711" }, { "type": "cds", "sequence": "contig_1", "start": 401569, "stop": 401871, "strand": "+", "frame": 1, "gene": null, "product": "putative MG243-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75439", "UniRef:UniRef90_P75439" ], "nt": "ATGCAGGTTAAAGTAATTGATGAAAACAGCAATACAACCGCTGTCCTTAACTGCGCTAAAGCGAAAACGCGAGCGCTAGCTTGGTTGTGTGATACAGTACTGTTAGCGATCTTACTCGCAATTATTTACGGCATTAGTAGCATTTTCATCAAAGAACAGTCCAGTGTGTTTTTAATTATGACGGTAAGTCAAGCAGTCTTGTGGTTGACTTACTTTGTCATTTTACCGGGTTTATGGAAGGGCAAAACGCTCTTTCGTGCTTTACTGGGTTTAAGTTTACTCATCTTTAAAAAACGCTTTTGA", "aa": "MQVKVIDENSNTTAVLNCAKAKTRALAWLCDTVLLAILLAIIYGISSIFIKEQSSVFLIMTVSQAVLWLTYFVILPGLWKGKTLFRALLGLSLLIFKKRF", "aa_hexdigest": "133ea20dfc5d0d5ac66f93d9121aa6fb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 401569, "stop": 402174, "observations": { "mutation": [ 401869, 401899 ], "stop": [ 401869, 401899 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75439", "query_cov": 1.0, "subject_cov": 0.44642857142857145, "identity": 1.0, "score": 186.0, "evalue": 6.22e-58, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MQVKVIDENSNTTAVLNCAKAKTRALAWLCDTVLLAILLAIIYGISSIFIKEQSSVFLIMTVSQAVLWLTYFVILPGLWKGKTLFRALLGLSLLIFKKRFWNLLVHELFLWVWYSVIFLALAIYFFVNRDDPKILQAFFDNQNSNLSWIFVKILLSVISVLQLVFVVYFCFSSQKQALQDLLSKSFMVQKAIKVKDCKSELKSTNTIKTHSDLPGDIDLEQLGD" }, "paralog": false, "identity": 0.9850746268656716, "score": 382.0, "evalue": 2.29e-137, "description": "Internal stop codon at 401869, 401899. Nonsense mutation around 401869, 401899.", "psc": { "uniref90_id": "UniRef90_P75439", "product": "Uncharacterized protein MG243 homolog", "uniref50_id": "UniRef50_P75439", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75439", "UniRef:UniRef50_P75439", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75439", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75439" ], "product": "Uncharacterized protein MG243 homolog" } }, "id": "GIHJHMOALN_738", "locus": "GCA009939745_00712" }, { "type": "cds", "sequence": "contig_1", "start": 402028, "stop": 402243, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAAGTGTTATTAGTGTCTTACAACTAGTATTTGTGGTGTATTTCTGTTTCAGCTCCCAAAAGCAAGCGTTGCAAGATTTGTTATCTAAGAGTTTTATGGTGCAAAAAACAATTAAAGTAAAGGATTGTAAAAGTGAATTGAAGTCCACTAACACTATAAAAACGCACTCTGATTTACCCGGCGACATTGATTTAGAACAATTAGGTGATTAA", "aa": "MLSVISVLQLVFVVYFCFSSQKQALQDLLSKSFMVQKTIKVKDCKSELKSTNTIKTHSDLPGDIDLEQLGD", "aa_hexdigest": "2defa3eb3389342dfea20488ba5def0e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7981.239799999999, "isoelectric_point": 6.53377857208252 }, "id": "GIHJHMOALN_739", "locus": "GCA009939745_00713" }, { "type": "cds", "sequence": "contig_1", "start": 402247, "stop": 403764, "strand": "+", "frame": 1, "gene": "uvrD", "product": "putative DNA helicase MPN_340", "db_xrefs": [ "COG:COG0210", "COG:L", "EC:5.6.2.4", "GO:0000725", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0033202", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "SO:0001217", "UniRef:UniRef50_P75438", "UniRef:UniRef90_P75438" ], "nt": "ATGGAACATTTAAATCAGGAACAGAAAGCTGCTGTTACTTGTGATAACGGTGTTAACGTGGTGTATTCCGGTGCTGGCACGGGTAAAACAACCGTTATTGCTGAACGCTTTGCTTACTTAGTCAATGAAAAAGGCGTTAATCCTCAAAGTATTTTGGCCTTTACCTTCACTGATAAGGCAGCGAGTGAAATGCGCCAACGCATTATTAAGTTAATTCCACAAAAAAGCTTGCAGGATCTGCACATCTATACCTTTCACAGTTTTGCCAACAGGTTTTTGCAAAAACATGGCAAAAGTGACTTTGCCATCTTGAGCGATTCTAACCGCTTCTTTAGTGATTATGAAATGGGTGATCAACTACAAACAGTGGTGGAAATTTATAAAAACAAAGTAGTTGATTTAGAACTTGACAACCTAGAGTATAACTCCGCTTTTAGAGATGCTTGTACAGATACTTTTAACGAGGATTTTAGTACCATCTCCAATGGTCAGTTTCGTAAACGGGCTGCGACTGCTTTACGGGCTTACCAGAACTATTTAATTACCAATAACCTATTTGATTTTAGCGATTTAATTATTGAAACTTGCCACCTTTTAAAAGGTAATAGTGAATTGTTGCAAGCATTTACCGAAAGTGTGCATTATATCTTAGTAGATGAATTTCAAGACACTAACCTAGCCCAGTACGAATTGGTAAAGTTACTAGCTACAACTCATCCTAATCTCTTTTTGGTGGGTGATAGCAACCAGATGATTTACGGTTGGCGGGGTGCTGTTGTAGAGATCTTTGAACTTTTAAAAAATGATTTTCAAACAGTCAAAGAGTTTTATACCACCCAAAACTACCGCAGCAGCCAAGCGGTGTTAGCAGTAGCGAATGATGTACTAACCGCAATTGCTAGAAAAGAGAGGAAAGCGCTCGTGTTGTTGCACTCTAGCATTGATTCTAAAGCTGTACCGGTACACTATAAGGCCAATTCTTTGAAAAACCAAGACCAGTGGATTATTTACCAAATGAAACAGTTGCATTTAAACAATGGTGTACCTTATGATCAGATGGCGGTGTTATTTCGTAAGAATAAACACCTTGACGCTTTTAGTCAAACAGTGCTGGAAGATGGTGATTTACCTTTAGCTAAATTAAACCTTTTAACAATTCACGCTGCCAAGGGTTTGGAATTTGAGGCAGTATTTGTTTATGGCTTGGTTGAACGCGCTTTTCCTAGTTTGCACTGGGATGGAAGTGATAAGCACAAGTTACTAGAAGAAATGAAACTGTTTTACGTAGCGATTACTCGTGCTAAACAGTTTTTATTTTTAGTTTCTGTTAGTGTGGAAAGTTTCAATGCGTATTATGAGCCGTCACGCTTTTTAAAACTAATTGAAAAGGAACATTTGCAAACACAAAAGGCAGCGTACTTTAAAGAACAATTAAAGACACAGCCAAAACCAGTTAATTTGTATACAGAGACAGAAAACGCGGAAAACTTACAAAAAGCCACTGACTCTAAAAAGTGA", "aa": "MEHLNQEQKAAVTCDNGVNVVYSGAGTGKTTVIAERFAYLVNEKGVNPQSILAFTFTDKAASEMRQRIIKLIPQKSLQDLHIYTFHSFANRFLQKHGKSDFAILSDSNRFFSDYEMGDQLQTVVEIYKNKVVDLELDNLEYNSAFRDACTDTFNEDFSTISNGQFRKRAATALRAYQNYLITNNLFDFSDLIIETCHLLKGNSELLQAFTESVHYILVDEFQDTNLAQYELVKLLATTHPNLFLVGDSNQMIYGWRGAVVEIFELLKNDFQTVKEFYTTQNYRSSQAVLAVANDVLTAIARKERKALVLLHSSIDSKAVPVHYKANSLKNQDQWIIYQMKQLHLNNGVPYDQMAVLFRKNKHLDAFSQTVLEDGDLPLAKLNLLTIHAAKGLEFEAVFVYGLVERAFPSLHWDGSDKHKLLEEMKLFYVAITRAKQFLFLVSVSVESFNAYYEPSRFLKLIEKEHLQTQKAAYFKEQLKTQPKPVNLYTETENAENLQKATDSKK", "aa_hexdigest": "fa02f073947d464e451bc536b7fcde2a", "start_type": "ATG", "rbs_motif": "AACAA", "psc": { "uniref90_id": "UniRef90_P75438", "query_cov": 1.0, "subject_cov": 0.9546313799621928, "identity": 0.996, "score": 983.0, "evalue": 0.0, "valid": true, "gene": "UvrD", "product": "Probable DNA helicase MPN_340", "ec_ids": [ "5.6.2.4" ], "uniref50_id": "UniRef50_P75438", "cog_id": "COG0210", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0033202", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0000725" ] }, "pscc": { "uniref50_id": "UniRef50_P75438", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75438" ], "product": "Probable DNA helicase MPN_340" }, "genes": [ "uvrD" ], "id": "GIHJHMOALN_740", "locus": "GCA009939745_00714" }, { "type": "cds", "sequence": "contig_1", "start": 403899, "stop": 404549, "strand": "+", "frame": 3, "gene": null, "product": "ATP-dependent helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013300ABE", "UniRef:UniRef90_UPI0013300ABE" ], "nt": "ATGGCATTTAACATTAGCACTCAACTCAATAAGGAACAACGAGCCGCTGTTACCTGTGGTAAGGGTGTCAACATTGTCTATTCTGGTGCTGGTACTGGTAAGACCACCATTATTTCCCAACGCTTTGCTTATTTATTTAACCAAAAGCGAATTAATCCTAGCAACATTTTGGCGTTAACCTATACAAGGAAAGCGGCGAGTGAGATGAAGCAGCGGATTTTGGAGCTGTTACCGGAAAAGTATCATAAGGATGTCAACATTTACACCTTCCACAGCTTTTGTAGCCGTTTTTTACGTGAAGAAGGGCAAAAAAACTTTGTGATTGATGATGACTTAAGTAACTTTCTAAAAGACTTTCTCAAAGAGAGTGAACTAAAAAGTCAAAAGGTATTACAAATTATTGACGGTTTTAAAAACGCTTACTTTGATTTTGATACCAACAGCTTAAAGGATGATGAAAGATTGGTCGAGTTGTGTGAGTTTCATTTAGACCCCCAAGAGCGTAACTTTCAACTTTTCAAAGAAACCGCTATTGCTGCTTTTGTTGAATATGAAAAAGGTAAACAACAGAACAATAAAATTGACTTTGCTGATTTATTAATTAAAACTTGCACTTTACTGAGCAAAGATCACAAATTATTGCGCAAGTGA", "aa": "MAFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALTYTRKAASEMKQRILELLPEKYHKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFLKESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCEFHLDPQERNFQLFKETAIAAFVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRK", "aa_hexdigest": "d196bf69d5447ba638200a87db8c29ec", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 403899, "stop": 404852, "observations": { "mutation": [ 404547, 404685 ], "stop": [ 404547, 404685 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013300ABE", "query_cov": 1.0, "subject_cov": 0.494279176201373, "identity": 1.0, "score": 429.0, "evalue": 7.38e-149, "gene_start": 1, "gene_end": 216, "reference_start": 1, "reference_end": 216, "reference_sequence": "MAFNISTQLNKEQRAAVTCGKGVNIVYSGAGTGKTTIISQRFAYLFNQKRINPSNILALTYTRKAASEMKQRILELLPEKYHKDVNIYTFHSFCSRFLREEGQKNFVIDDDLSNFLKDFLKESELKSQKVLQIIDGFKNAYFDFDTNSLKDDERLVELCEFHLDPQERNFQLFKETAIAAFVEYEKGKQQNNKIDFADLLIKTCTLLSKDHKLLRKWSKKFQYILGDEFQDTNQIQYELIKMLASHHQNLFLVGDNNQMIYRWRGAVSDIIDSLKSDFKVRPENEFYITQNYRCDQNILTVANSILGTIYAKENQPDSTKGFLFSAIKSNRLPVYFQASSVEGQHSWIINKIKNLHKNHGIQYKDMAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLALKSLRVWTNVLNKSH" }, "paralog": false, "identity": 0.9936908517350158, "score": 627.0, "evalue": 5.25e-229, "description": "Internal stop codon at 404547, 404685. Nonsense mutation around 404547, 404685.", "psc": { "uniref90_id": "UniRef90_UPI0013300ABE", "product": "ATP-dependent helicase", "uniref50_id": "UniRef50_UPI0013300ABE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013300ABE", "UniRef:UniRef50_UPI0013300ABE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013300ABE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013300ABE" ], "product": "ATP-dependent helicase" } }, "id": "GIHJHMOALN_741", "locus": "GCA009939745_00715" }, { "type": "cds", "sequence": "contig_1", "start": 404994, "stop": 405185, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTTGTTTCGCACCAACAGAAACATGGATTCGATGACTGAAGCGCTTGAAGCTGATGGCAGTATTCCCCTTAAGCAAAATAAGGGTTTTTTTAAACAGTTAGAGACTTTTAAAAAGGTATTAGTGGCACTCATAACTAGGTCAAACTATGACATTAAACTAGCTTTAAAAAGTTTGCGGGTTTGA", "aa": "MAILFRTNRNMDSMTEALEADGSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLALKSLRV", "aa_hexdigest": "eca3d2b96738a907cdf33187523e4ba2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7218.467499999999, "isoelectric_point": 9.991777610778808 }, "id": "GIHJHMOALN_742", "locus": "GCA009939745_00716" }, { "type": "cds", "sequence": "contig_1", "start": 405225, "stop": 406004, "strand": "+", "frame": 3, "gene": null, "product": "ATP-dependent helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310682", "UniRef:UniRef90_UPI0013310682" ], "nt": "GTGAATTTAGATAAAATCTTGCAAAACTTAGAGCAAGCCATTTTTTTAGATGAGCATACTCGGGGTGAGTTGACGGAAGCTGCTAAAGTTTTTACGCGTTTAATTAAGTTTGTTGAAGAACAACAGTTTGAAGCTTTATTAGCCTTTACTTTTGAGGCATTAAATACTGATCAGTTTGTCTGCAACTTTATTTTTAGAACATTGCAAAAACTGCAAACTGAAAATAAAAACTTCACCATTACCGATTTTATTAACGAGTTAAGGTTCCAACAAGATGAGTTAAAGCAGTCCAAAGATAATGTGATTAATTTAATTACAGTGCACTCTGCTAAGGGCTTGGAATTTGAAGCTGTCTTTATTTATGGGATCAATCAAGACAACTTTCCGCTCAAATCGAAGAATCAAATAATAGATCTACAAAGAGAATTGGATGAACTACGCCTTTTTTATGTGGCCTTGACAAGGGCAAAGAAATACTTGTTTTTACTTTCAGTTTATCAAATGAGTGGTGAAATTATTTACCCCTCTAAATTCATTCGTTTTATCAATAAAGAGGATAGACTAGAAATTGCCACCATTAACCATAAAGTTCACCAGGATGATGAATTCTTCGATTCTTCTAAAACAGAAGACTATCAAAAAAAGTATTTAACAGAAAATACGGATTTTGTTAATGGTGACTCTGTTAGCCACCGCACTTATGGTAAGGGCGTTGTTGTTGAAGTTAGAGAAGATGCAGTCTGTGTCGCTTTTAAAAACAGTAAGTACGGACAGAGGTGA", "aa": "MNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFVEEQQFEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVINLITVHSAKGLEFEAVFIYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFLLSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVHQDDEFFDSSKTEDYQKKYLTENTDFVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQR", "aa_hexdigest": "243e4dd0ac70085592bd8a9bfcd458a3", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 405045, "stop": 406046, "observations": { "mutation": [ 405183, 406002 ], "stop": [ 405183, 406002 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013310682", "query_cov": 1.0, "subject_cov": 0.7777777777777778, "identity": 0.996, "score": 501.0, "evalue": 5.51e-178, "gene_start": 1, "gene_end": 259, "reference_start": 61, "reference_end": 319, "reference_sequence": "LEADCSIPLKQNKGFFKQLETFKKVLVALITRSNYDIKLALKSLRVWPNVLNKSLTVNEQVNLDKILQNLEQAIFLDEHTRGELTEAAKVFTRLIKFVEEQQFEALLAFTFEALNTDQFVCNFIFRTLQKLQTENKNFTITDFINELRFQQDELKQSKDNVINLITVHSAKGLEFEAVFIYGINQDNFPLKSKNQIIDLQRELDELRLFYVALTRAKKYLFLLSVYQMSGEIIYPSKFIRFINKEDRLEIATINHKVHQDDEFFDSSKTEDYQKKYLTENTDFVNGDSVSHRTYGKGVVVEVREDAVCVAFKNSKYGQRWIVKNHRDLVKAVY" }, "paralog": false, "identity": 0.990990990990991, "score": 642.0, "evalue": 6.91e-236, "description": "Internal stop codon at 405183, 406002. Nonsense mutation around 405183, 406002.", "psc": { "uniref90_id": "UniRef90_UPI0013310682", "product": "ATP-dependent helicase", "uniref50_id": "UniRef50_UPI0013310682", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013310682", "UniRef:UniRef50_UPI0013310682" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013310682", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013310682" ], "product": "ATP-dependent helicase" } }, "id": "GIHJHMOALN_743", "locus": "GCA009939745_00717" }, { "type": "cds", "sequence": "contig_1", "start": 406535, "stop": 407167, "strand": "+", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N2BQM8" ], "nt": "TTGCGCGATATCTTCGATGATATTGAAAAGTCTACTGAACTAGGTGATGGAAGAAGTAAAGAATCCTTTAAAGGTCTTTTCAAAGACTTTAATGTTTCTGAGGTTAAGTTAGGTTCTACTTTAACGATCCGAACAGAGAAATTAAAAGAACTTTTAACAAGCATTGACACAATGGAGCTCGATGAATTTGAGAAAAATTCTATCGATGCATTTGGTGATGCATATGAGTTTTTAATTTCCATGTATGCACAAAATGCTGGAAAAAGTGGGGGTGAGTTTTTTACACCCCAAGATGTATCTGAACTTCTCGCTAGAATTGCGATTGGCAAAAAGGACACCGTTGATGATGTTTATGACATGGCTTGTGGCAGTGGTTCTTTACTTTTACAAGTAATTAAAGTTTTAGGTAAAGAAAAAACTTCACTGGTTAGCTATTACGGTCAGGAAATAAACCACACAACTTACAACCTTTGTCGGATGAATATGATTCTCCACAATATTGATTACGCTAATTTCAATATTATTAACGCTGATACTTTAACAACGAAGGAGTGGGAGAAGCATTACGTAAATTGCTCAAATGAAAATGGGTTTGAAGTTGTGGTTTCCAATCCACCATATTCCATTAGTTGA", "aa": "MRDIFDDIEKSTELGDGRSKESFKGLFKDFNVSEVKLGSTLTIRTEKLKELLTSIDTMELDEFEKNSIDAFGDAYEFLISMYAQNAGKSGGEFFTPQDVSELLARIAIGKKDTVDDVYDMACGSGSLLLQVIKVLGKEKTSLVSYYGQEINHTTYNLCRMNMILHNIDYANFNIINADTLTTKEWEKHYVNCSNENGFEVVVSNPPYSIS", "aa_hexdigest": "7ffbfab5b7e041f92c69c9f8afe28638", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_N2BQM8", "query_cov": 0.8857142857142857, "subject_cov": 0.9946524064171123, "identity": 0.5429999999999999, "score": 176.0, "evalue": 8.36e-53, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_N2BQM8", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_N2BQM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N2BQM8" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "GIHJHMOALN_744", "locus": "GCA009939745_00718" }, { "type": "cds", "sequence": "contig_1", "start": 407225, "stop": 407818, "strand": "+", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A132HPU2" ], "nt": "TTGGCACCTAATTCAAAAGCTGATTTAGCTTTTGTTTTACATGCTCTATATGTATTAGGTCAAGAAGGTACAGCAGCAATTGTTTGTTTCCCTGGAATCTTGTACCGTGAAGGTAAAGAGCAAACCATTAGAAAATACCTAGTTGATCAAAACTTTGTTGATGCAGTGATCCAATTACCAAGTAATCTTTTTAGCACAACATCAATCGCTACTTCAATTCTTGTTCTCAAGAAAAATCGTGATAAAAAAGATCCCATTTTCTTCATTGATGGTTCTAACGAATTTGTTAGAGAAAAGAAGAATAACCGCTTAAGTCCAAAAAATATTGAAAAGATTGTTGATTGCTTTAATAGCAAAAAAGAAGAAGCTAATTTTGCAAAGGCGGTTGAAAGGGACAAAATTAGGGAATCTAACTACGACCTTACAGTAGGTAAATACGTAAACTCAGAGGCTGAAAAGGAAGAATTAGATATTAAGGTTTTGAATCACTCTATTGATGAAATAGTAGATAAACAAAAAGATCTAAGAACTAAAATTAAGGATATTATTCAGGATATCAAAGTAGATTTTGACAACATTGATATTAATAACTAG", "aa": "MAPNSKADLAFVLHALYVLGQEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVDAVIQLPSNLFSTTSIATSILVLKKNRDKKDPIFFIDGSNEFVREKKNNRLSPKNIEKIVDCFNSKKEEANFAKAVERDKIRESNYDLTVGKYVNSEAEKEELDIKVLNHSIDEIVDKQKDLRTKIKDIIQDIKVDFDNIDINN", "aa_hexdigest": "b7090f8053296bb5eac7d605b83109b1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028898C0F", "query_cov": 0.9593908629441624, "subject_cov": 0.8873239436619719, "identity": 0.508, "score": 179.0, "evalue": 1.45e-53, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A132HPU2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A132HPU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A132HPU2" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "GIHJHMOALN_745", "locus": "GCA009939745_00719" }, { "type": "cds", "sequence": "contig_1", "start": 407888, "stop": 408067, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGCAAATTAAAACTTACAAAATCAAGGATATTTGCGAGATCTCAAGAGGCAAAGCAATTACTAAAAAATACATTAAGGATAATCCTGGTCAATATCCGGTTTATTCATCTACTACTGCTAATAACGGTGAAATTGGCAGAATAAAGGATTACGATCTTGATGGCGAATACGTTACCTGA", "aa": "MQIKTYKIKDICEISRGKAITKKYIKDNPGQYPVYSSTTANNGEIGRIKDYDLDGEYVT", "aa_hexdigest": "ab2463771057210eb59fcb3706209e77", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 60, "aa_cov": 0.9491525423728814, "hmm_cov": 0.9833333333333333, "evalue": 7.439818796956333e-12, "score": 41.26675796508789, "start": 4, "stop": 59 } ], "seq_stats": { "molecular_weight": 6735.562900000004, "isoelectric_point": 8.708276557922364 }, "id": "GIHJHMOALN_746", "locus": "GCA009939745_00720" }, { "type": "cds", "sequence": "contig_1", "start": 408197, "stop": 408610, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "ATGGAGGCTCCTAAATTTGTTAATAATGCTTGTCCTATTCCTAACCTCAATTTATCAAGAACTGAGGAAATTGAATTAGATTTCCCACCCTTGCAAATTCAGCAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATAACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGA", "aa": "MEAPKFVNNACPIPNLNLSRTEEIELDFPPLQIQQKIATILDTFTELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYIT", "aa_hexdigest": "a6da9a14205d811eaf0fab804d797772", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 62, "aa_cov": 0.4233576642335766, "hmm_cov": 0.9838709677419355, "evalue": 1.1300390846313142e-21, "score": 73.21320343017578, "start": 80, "stop": 137 } ], "seq_stats": { "molecular_weight": 15855.740199999982, "isoelectric_point": 5.107972526550292 }, "id": "GIHJHMOALN_747", "locus": "GCA009939745_00721" }, { "type": "cds", "sequence": "contig_1", "start": 408797, "stop": 408964, "strand": "+", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00190F3D87", "UniRef:UniRef90_UPI00190F3D87" ], "nt": "ATGGCTGAAATTGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAATTTCTTATTTAATTGA", "aa": "MAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFN", "aa_hexdigest": "bbbc1991845ad67ac2a240d1c026608d", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 408650, "stop": 409009, "observations": { "mutation": [ 408962 ], "stop": [ 408962 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00190F3D87", "query_cov": 1.0, "subject_cov": 0.46218487394957986, "identity": 1.0, "score": 114.0, "evalue": 1.06e-31, "gene_start": 1, "gene_end": 55, "reference_start": 50, "reference_end": 104, "reference_sequence": "GKCNASQDCGVLKVKNKKICTKFLSFLLKIEAPKFVHNLASRPKLSQKVMAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIELRKKQLDYYQNFLFNWVQEQKKNSLSTNLN" }, "paralog": false, "identity": 0.9831932773109243, "score": 231.0, "evalue": 4.77e-80, "description": "Internal stop codon at 408962. Nonsense mutation around 408962.", "psc": { "uniref90_id": "UniRef90_UPI00190F3D87", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00190F3D87", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00190F3D87", "EC:3.1.21.3", "UniRef:UniRef50_UPI00190F3D87", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00190F3D87", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00190F3D87" ], "product": "restriction endonuclease subunit S" } }, "id": "GIHJHMOALN_748", "locus": "GCA009939745_00722" }, { "type": "cds", "sequence": "contig_1", "start": 409232, "stop": 409882, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_344", "db_xrefs": [ "RefSeq:WP_014325475.1", "SO:0001217", "UniParc:UPI0001DD653A", "UniRef:UniRef100_A0AAX0SQN8", "UniRef:UniRef50_P75100", "UniRef:UniRef90_P75434" ], "nt": "ATGCCTAAAATTATTTCTAAACGAGAATTCAACATTCTTAGAAAAACAAAAGAAGAAATCTTTTCTCAAATTGTTTACACTAAGAAGAGAAACGGTCTGCACAAGGCTGCCAAAGAATATTACTTCAAGGACAAAGACTTTACAATAATAGAAGATCAACAGGATCGACCTGATAAACCTGAGGAACTGGACACACCGGATATTCCAAAGCCACCAAAACCTCCCAAGGGTCCTGATCAACCCGAGGAACCAGGCCAACCAGGTGGTCCCGATGATCCTAATTCAGGAAATAAAAAGATGCCAAAGCCAGACGAATTTGTAACCCACAGACAACTTCAAGAATTTAAGAAGGATCTTTTAGTGGAACTTCACGAAATCTTTCCTACAAAACCTGAATTAAAACGGGTTGAAGAAAAAGTTGATGTTCTGTTTGAACTTCAAAAAGCTCAGGGTGAACAAATAAGAATCCAAGGTGAACAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGGGCGAAACCCTACAGCTAATCTTGCAAACACTACAAAAGATGAACGATCGTCTGGATAAGATCGAAGGTAAAATGGACAAAATGGAAAGTAGAATGGATAAAATGGAAACTCGCTTAGACAAACTAGAGTCCAAATAA", "aa": "MPKIISKREFNILRKTKEEIFSQIVYTKKRNGLHKAAKEYYFKDKDFTIIEDQQDRPDKPEELDTPDIPKPPKPPKGPDQPEEPGQPGGPDDPNSGNKKMPKPDEFVTHRQLQEFKKDLLVELHEIFPTKPELKRVEEKVDVLFELQKAQGEQIRIQGEQIKELKVEQKAQGETLQLILQTLQKMNDRLDKIEGKMDKMESRMDKMETRLDKLESK", "aa_hexdigest": "34ea9fd1a79390b9fa9a44c828a1dc24", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD653A", "ncbi_nrp_id": "WP_014325475.1", "uniref100_id": "UniRef100_A0AAX0SQN8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD653A", "RefSeq:WP_014325475.1", "UniRef:UniRef100_A0AAX0SQN8" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQN8", "uniref90_id": "UniRef90_P75434" }, "psc": { "uniref90_id": "UniRef90_P75434", "product": "UPF0134 protein MPN_344", "uniref50_id": "UniRef50_P75100" }, "pscc": { "uniref50_id": "UniRef50_P75100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75100" ], "product": "UPF0134 protein MPN_013" }, "genes": [], "id": "GIHJHMOALN_749", "locus": "GCA009939745_00723" }, { "type": "cds", "sequence": "contig_1", "start": 411175, "stop": 411447, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACAACCAAACGATCGAAGAATATGAAAAGTTTCAAGCGGGTTCTGTTAGCGAAACTGAGAACACAAATGATCTTAAAGAAAAGATATTAGACGATAGTACCGCCACACGTGCAATTGTTACTACTATTCATAAACTTAAGAGGTTAATAGATCAGAGAAGTAAGCTTAAAGACGAAGATCTTAAAAAGAAAAACATCGTTCTTATATTCGATGAATGTCATCGATCTCAATTTGGAAAAATGAAACAAGAAATCGATGAGTTTTTTTAA", "aa": "MDNQTIEEYEKFQAGSVSETENTNDLKEKILDDSTATRAIVTTIHKLKRLIDQRSKLKDEDLKKKNIVLIFDECHRSQFGKMKQEIDEFF", "aa_hexdigest": "552960d73368a392dc7a5ca5419658c9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10608.871499999996, "isoelectric_point": 5.391087532043457 }, "id": "GIHJHMOALN_750", "locus": "GCA009939745_00724" }, { "type": "cds", "sequence": "contig_1", "start": 411577, "stop": 411924, "strand": "+", "frame": 1, "gene": null, "product": "Putative type I restriction enzyme MpnIIP endonuclease subunit middle part", "db_xrefs": [ "RefSeq:WP_010874702.1", "SO:0001217", "UniParc:UPI000013A844", "UniRef:UniRef100_P75432", "UniRef:UniRef50_P75432", "UniRef:UniRef90_P75432" ], "nt": "ATGTCTGATGCTTTGGATGACGAAAATGTACTTTGTTTTAGGTATAAGAGTTATTACATTAAAGATGATGGAAGCCGAATTGATACATTAAATGAAGAGAGAATTAAGCATATTGTTGATTTCACTTTAGATAAATATCTAGATGCAACACAAAATACTCCGAAATTTGATAATTTTGAAAATCCTGTAAAAGAAGGGTTTAATTCGATTTTTGCCTGTGAGAGTAAAGAAGCAGCAATCGAATACTATAAAGAATTCAAAAAACAAATTGAACAAAAGAAGCTACCAATTAAAATTGCAAGCATTATAGCGCAAACGAAGGTAGTGATGAACAAGAAATTAGTGTAA", "aa": "MSDALDDENVLCFRYKSYYIKDDGSRIDTLNEERIKHIVDFTLDKYLDATQNTPKFDNFENPVKEGFNSIFACESKEAAIEYYKEFKKQIEQKKLPIKIASIIAQTKVVMNKKLV", "aa_hexdigest": "baa01d1bcd6606b7f793ac25aacdf3f2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A844", "ncbi_nrp_id": "WP_010874702.1", "uniref100_id": "UniRef100_P75432", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A844", "RefSeq:WP_010874702.1", "UniRef:UniRef100_P75432" ] }, "ips": { "uniref100_id": "UniRef100_P75432", "uniref90_id": "UniRef90_P75432" }, "psc": { "uniref90_id": "UniRef90_P75432", "product": "Putative type I restriction enzyme MpnIIP endonuclease subunit middle part", "uniref50_id": "UniRef50_P75432" }, "pscc": { "uniref50_id": "UniRef50_P75432", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75432" ], "product": "Putative type I restriction enzyme MpnIIP endonuclease subunit middle part" }, "genes": [], "id": "GIHJHMOALN_751", "locus": "GCA009939745_00725" }, { "type": "cds", "sequence": "contig_1", "start": 413307, "stop": 413441, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574953.1", "SO:0001217", "UniParc:UPI0001DD653D", "UniRef:UniRef100_A0AB38W8M6", "UniRef:UniRef50_A0AB38W8M6", "UniRef:UniRef90_A0AB38W8M6" ], "nt": "ATGGAAAAGAAAAATAAAAAAAATACTGCAACCGTTCCCACCAAAGAACAATTGGATGGCATGTTTAAATCTCTTGAAAAAGAAGAGAACTATGATGTCTTTGTTCAAGTCATAAAAGCTTTAGGTAAGATGTAG", "aa": "MEKKNKKNTATVPTKEQLDGMFKSLEKEENYDVFVQVIKALGKM", "aa_hexdigest": "9b3c3574738bab55abda7bca2ce6156a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD653D", "ncbi_nrp_id": "WP_014574953.1", "uniref100_id": "UniRef100_A0AB38W8M6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD653D", "RefSeq:WP_014574953.1", "UniRef:UniRef100_A0AB38W8M6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8M6", "uniref90_id": "UniRef90_A0AB38W8M6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8M6", "uniref50_id": "UniRef50_A0AB38W8M6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8M6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8M6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5089.902800000002, "isoelectric_point": 9.050087928771973 }, "id": "GIHJHMOALN_752", "locus": "GCA009939745_00726" }, { "type": "cds", "sequence": "contig_1", "start": 413444, "stop": 413998, "strand": "+", "frame": 2, "gene": null, "product": "Death-on-curing family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S2Z8", "UniRef:UniRef90_A0AAX0S2Z8" ], "nt": "GTGCGTGAAATAAACGTTTATTATTTAAGCAACGACTCAAAAAGTGATAAAGACGATATCGATTATTTAATAGAAGAACTTTTAGTTGAAGGTTCTCGTTTTAAAAATAGTCTTGTTACGTTTTTAGAAAAAGAAAAAGACTGCCAATCTTGCATTTGTTTATTTAAATCTAAGCTTTCTTCTTCATTCGAAATAAGAGTCAAATTTAATGAATTAAATTTAGATGCAAGTGATGAAAAAACTAAGGAATTTTTAAGATACATAGTTAGTAAATCAATAAACAATGCAAAATTCCTATCTTCTGAAGACTTTGATGACAAAAAAGAAGACGAAATTCTAAGCATCATTGAGAATACCTTTAATAGTTATTGCTACAAAAACGATTTACTAAGTCCAAGTTTGTATTGTAAAGAAGGCCGCTTGAACATAATTGATTTAATTAACGATATTTTTTGCAAGGTTTTAACTTTTCATAAATTGTCGAACGGAAATAAAAGACTAGCTACAGCCTTATTAGTTAATTTTCTTTACACTTTTGGATATTATTTCAAATGA", "aa": "MREINVYYLSNDSKSDKDDIDYLIEELLVEGSRFKNSLVTFLEKEKDCQSCICLFKSKLSSSFEIRVKFNELNLDASDEKTKEFLRYIVSKSINNAKFLSSEDFDDKKEDEILSIIENTFNSYCYKNDLLSPSLYCKEGRLNIIDLINDIFCKVLTFHKLSNGNKRLATALLVNFLYTFGYYFK", "aa_hexdigest": "499da2d38f605189eaa291819c797a1c", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 413444, "stop": 414178, "observations": { "mutation": [ 413996, 414158 ], "stop": [ 413996, 414158 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAX0S2Z8", "query_cov": 1.0, "subject_cov": 0.7540983606557377, "identity": 0.995, "score": 359.0, "evalue": 1.79e-124, "gene_start": 1, "gene_end": 184, "reference_start": 1, "reference_end": 184, "reference_sequence": "MREINVYYLSNDSKSDKDDIDYLIEELLVEGSRFKNSLVTFLEKEKDCQSCICLFKSKLSSSFEIRVKFNELNLDASDEKTKEFLRYIVSKSINNAKFLSSEDFDDKKEDEILSIIENTFNSYCYKSDLLSPSLYCKEGRLNIIDLINDIFCKVLTFHKLSNGNKRLATALLVNFLYTFGYYFKWTIPLKKEEYFKYAHMIKNFVVSFEEHKRENRDEFLAKQTKKFIMDNILLALNFWETKLN" }, "paralog": false, "identity": 0.9836065573770492, "score": 478.0, "evalue": 1.03e-173, "description": "Internal stop codon at 413996, 414158. Nonsense mutation around 413996, 414158.", "psc": { "uniref90_id": "UniRef90_A0AAX0S2Z8", "product": "Death-on-curing family protein", "uniref50_id": "UniRef50_A0AAX0S2Z8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAX0S2Z8", "UniRef:UniRef50_A0AAX0S2Z8" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAX0S2Z8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S2Z8" ], "product": "Death-on-curing family protein" } }, "id": "GIHJHMOALN_753", "locus": "GCA009939745_00727" }, { "type": "cds", "sequence": "contig_1", "start": 414041, "stop": 414160, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAAAAACTTTGTAGTTAGTTTTGAAGAACATAAAAGAGAAAACAGGGATGAATTTCTAGCAAAGCAAACAAAGAAATTTATCATGGACAACATCCTTTTAGCTCTCAATTTTTGA", "aa": "MIKNFVVSFEEHKRENRDEFLAKQTKKFIMDNILLALNF", "aa_hexdigest": "2f3b8c462bd921c9950515ac9f9d514c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4758.521099999999, "isoelectric_point": 8.162164878845214 }, "id": "GIHJHMOALN_754", "locus": "GCA009939745_00728" }, { "type": "cds", "sequence": "contig_1", "start": 414216, "stop": 414710, "strand": "+", "frame": 3, "gene": "fAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "db_xrefs": [ "COG:COG0212", "COG:H", "EC:6.3.3.2", "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872", "RefSeq:WP_010874704.1", "SO:0001217", "UniParc:UPI00001394EC", "UniRef:UniRef100_P75430", "UniRef:UniRef50_P75430", "UniRef:UniRef90_P75430" ], "nt": "ATGGACAAAAATGCCTTAAGAAAACAAATTCTGCAAAAAAGAATGGCATTAAGTACCATTGAAAAAAGTCACTTAGATCAAAAGATTAATCAAAAATTAGTTGCTTTTTTAACTCCTAAGCCATGCATTAAAACAATTGCACTTTATGAACCCATTAAAAATGAGGTTACTTTTGTTGACTTCTTCTTTGAGTTTTTAAAGATTAACCAAATAAGAGCTGTTTACCCCAAGGTAATAAGTGATACCGAAATTATCTTTATTGATCAGGAGACAAATACATTCGAACCTAATCAAATAGACTGCTTCCTAATACCATTGGTAGGCTTTAATAAAGACAATTACCGTCTAGGCTTTGGCAAGGGCTATTATGACCGTTATTTAATGCAATTAACTAGACAACAACCTAAAATAGGAATAGCGTACAGTTTCCAAAAAGGTGATTTTTTAGCCGATCCTTGGGATGTACAACTAGACTTAATTATTAATGATGAATAG", "aa": "MDKNALRKQILQKRMALSTIEKSHLDQKINQKLVAFLTPKPCIKTIALYEPIKNEVTFVDFFFEFLKINQIRAVYPKVISDTEIIFIDQETNTFEPNQIDCFLIPLVGFNKDNYRLGFGKGYYDRYLMQLTRQQPKIGIAYSFQKGDFLADPWDVQLDLIINDE", "aa_hexdigest": "a9956fc88c9aea3f4484912cbd983518", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394EC", "ncbi_nrp_id": "WP_010874704.1", "uniref100_id": "UniRef100_P75430", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394EC", "RefSeq:WP_010874704.1", "UniRef:UniRef100_P75430" ] }, "ips": { "uniref100_id": "UniRef100_P75430", "uniref90_id": "UniRef90_P75430" }, "psc": { "uniref90_id": "UniRef90_P75430", "gene": "FAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "ec_ids": [ "6.3.3.2" ], "uniref50_id": "UniRef50_P75430", "cog_id": "COG0212", "cog_category": "H", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75430", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75430" ], "product": "5-formyltetrahydrofolate cyclo-ligase" }, "genes": [ "fAU1" ], "id": "GIHJHMOALN_755", "locus": "GCA009939745_00729" }, { "type": "cds", "sequence": "contig_1", "start": 414700, "stop": 415545, "strand": "+", "frame": 1, "gene": "ymdB", "product": "Putative phosphatase/phosphodiesterase MPN_349", "db_xrefs": [ "COG:COG1692", "COG:T", "EC:3.1.-.-", "GO:0004113", "GO:0046872", "KEGG:K09769", "RefSeq:WP_010874705.1", "SO:0001217", "UniParc:UPI00001394F4", "UniRef:UniRef100_P75429", "UniRef:UniRef50_P75429", "UniRef:UniRef90_P75429" ], "nt": "ATGATGAATAGCATTAAATTCATTTTCCTAGGTGACGTCTATGGCAAGGCCGGACGTAATATTATTAAGAATAATCTAGCCCAACTCAAATCAAAGTATCAAGCTGATTTAGTCATTGTTAATGCCGAAAATACGACTCACGGTAAAGGACTTAGTTTAAAGCACTATGAGTTTCTAAAAGAGGCAGGCGTTAATTACATTACTATGGGTAATCATACCTGGTTTCAGAAGCTAGATTTAGCTGTTGTAATTAATAAAAAGGACTTAGTGCGTCCTTTAAACTTAGACACCAGTTTTGCTTTTCATAACTTAGGGCAAGGTAGTCTTGTATTTGAATTTAATAAAGCAAAGATTAGGATAACTAATTTACTGGGTACTAGTGTGCCCTTACCCTTTAAAACAACTAATCCCTTTAAAGTTTTAAAAGAACTAATTTTAAAGCGCGATTGTGATCTTCATATAGTTGATTTTCACGCTGAAACCACCAGTGAAAAAAACGCCTTTTGCATGGCTTTTGATGGTTATGTCACAACCATTTTCGGCACCCACACCCACGTGCCTAGTGCTGATTTAAGAATCACCCCCAAAGGTAGTGCTTACATTACCGATGTCGGGATGTGTGGTCCGGGATTTGGTAGTGTTATTGGTGCTAACCCCGAACAGTCGATCAGACTCTTTTGTGCTGGTAGCAGAGAACACTTTGAGGTATCTAAATGCGGAGCACAACTCAATGGTGTCTTCTTTGAGGTTGATGTAAATACAAAAAAGGTGATTAAAACAGAGGCGATTAGGATTGTGGAGGACGATCCTAGATACTTAAAACAAGACTACTTTAACCTAATTTAA", "aa": "MMNSIKFIFLGDVYGKAGRNIIKNNLAQLKSKYQADLVIVNAENTTHGKGLSLKHYEFLKEAGVNYITMGNHTWFQKLDLAVVINKKDLVRPLNLDTSFAFHNLGQGSLVFEFNKAKIRITNLLGTSVPLPFKTTNPFKVLKELILKRDCDLHIVDFHAETTSEKNAFCMAFDGYVTTIFGTHTHVPSADLRITPKGSAYITDVGMCGPGFGSVIGANPEQSIRLFCAGSREHFEVSKCGAQLNGVFFEVDVNTKKVIKTEAIRIVEDDPRYLKQDYFNLI", "aa_hexdigest": "f7efcd5505e4e1a5cd1366968b50b3de", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394F4", "ncbi_nrp_id": "WP_010874705.1", "uniref100_id": "UniRef100_P75429", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394F4", "RefSeq:WP_010874705.1", "UniRef:UniRef100_P75429" ] }, "ips": { "uniref100_id": "UniRef100_P75429", "uniref90_id": "UniRef90_P75429" }, "psc": { "uniref90_id": "UniRef90_P75429", "gene": "YmdB", "product": "Putative phosphatase/phosphodiesterase MPN_349", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75429", "cog_id": "COG1692", "cog_category": "T", "kegg_orthology_id": "K09769", "go_ids": [ "GO:0004113", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75429", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75429" ], "product": "Putative phosphatase/phosphodiesterase MPN_349" }, "genes": [ "ymdB" ], "id": "GIHJHMOALN_756", "locus": "GCA009939745_00730" }, { "type": "cds", "sequence": "contig_1", "start": 415739, "stop": 415921, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGCTAAAATAAAGAAGGTAACTAAGCTAGCTAAAGAAACGTACTTGGTTATTAAAGTAACTAGCAATCACAAAACTAAGCAAATAACAAATCACCACAGCGAAATTGCCAGCAAAGAACCGCCTGTAGTAGCGATGGCTTTACCACCTTTAAACTTAAAGTACAGTGGAAAAATATGA", "aa": "MIAKIKKVTKLAKETYLVIKVTSNHKTKQITNHHSEIASKEPPVVAMALPPLNLKYSGKI", "aa_hexdigest": "7e73270642d0ba9ca62ab575e34a0813", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6680.9648, "isoelectric_point": 10.06707668304443 }, "id": "GIHJHMOALN_757", "locus": "GCA009939745_00731" }, { "type": "cds", "sequence": "contig_1", "start": 415834, "stop": 416256, "strand": "-", "frame": 1, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "db_xrefs": [ "COG:COG0344", "COG:I", "EC:2.3.1.15", "EC:2.3.1.275", "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772", "KEGG:K08591", "SO:0001217", "UniRef:UniRef50_P75428", "UniRef:UniRef90_P75428" ], "nt": "ATGAACGCAGCTAGCGCCATCGCTCTACTTATTGTCTTTAGCCTTGTTATTGGCTATTTAATGGGTTCAGTGATGTTTGCTGATGTTTTTGGCAAGATCTTAAACAAAGATGTCAGGAAATTGGGCTCCAAGAATCCGGGGGCTACTAACTCAATCCGTGTTTTTGGTTTGAAAATAGGCTTCTTAGTTGGTTTATGTGATGCTTTAAAGGGGTTTTTGGCGTTTGTTTTCAGCTTTTTAATCTTTAGCTTTTGGTTACAACAATACCTTAATGTAAACCAGTATCAAAAGGTTTATTACCTCACTTATTTAAGTTGTTTTGCAGCTACCATTGGTCATATTTTTCCACTGTACTTTAAGTTTAAAGGTGGTAAAGCCATCGCTACTACAGGCGGTTCTTTGCTGGCAATTTCGCTGTGGTGA", "aa": "MNAASAIALLIVFSLVIGYLMGSVMFADVFGKILNKDVRKLGSKNPGATNSIRVFGLKIGFLVGLCDALKGFLAFVFSFLIFSFWLQQYLNVNQYQKVYYLTYLSCFAATIGHIFPLYFKFKGGKAIATTGGSLLAISLW", "aa_hexdigest": "0c841b5f00599d8a747333815bfa2b44", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "plsY" ], "truncated": "5-prime", "pseudogene": { "start": 415537, "stop": 416256, "observations": { "mutation": [ 415605, 415629, 415812, 415836 ], "stop": [ 415605, 415629, 415812, 415836 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75428", "query_cov": 1.0, "subject_cov": 0.5857740585774058, "identity": 1.0, "score": 270.0, "evalue": 3.75e-90, "gene_start": 1, "gene_end": 140, "reference_start": 1, "reference_end": 140, "reference_sequence": "MNAASAIALLIVFSLVIGYLMGSVMFADVFGKILNKDVRKLGSKNPGATNSIRVFGLKIGFLVGLCDALKGFLAFVFSFLIFSFWLQQYLNVNQYQKVYYLTYLSCFAATIGHIFPLYFKFKGGKAIATTGGSLLAISLWWFVICLVLWLLVTLITKYVSLASLVTFFILAIIILVPWLDYLYFFKPNPINAISYQNDWYIILFFVLWYWPLTIAVFWLHRKNIHRLLNKTENKVTQLN" }, "paralog": false, "identity": 0.9832635983263598, "score": 464.0, "evalue": 6.86e-169, "description": "Internal stop codon at 415605, 415629, 415812, 415836. Nonsense mutation around 415605, 415629, 415812, 415836.", "psc": { "uniref90_id": "UniRef90_P75428", "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "ec_ids": [ "2.3.1.15", "2.3.1.275" ], "uniref50_id": "UniRef50_P75428", "cog_id": "COG0344", "cog_category": "I", "kegg_orthology_id": "K08591", "go_ids": [ "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75428", "EC:2.3.1.15", "EC:2.3.1.275", "UniRef:UniRef50_P75428", "COG:COG0344", "COG:I", "KEGG:K08591", "GO:0005886", "GO:0006654", "GO:0008374", "GO:0008654", "GO:0043772" ] }, "pscc": { "uniref50_id": "UniRef50_P75428", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75428" ], "product": "Glycerol-3-phosphate acyltransferase" } }, "id": "GIHJHMOALN_758", "locus": "GCA009939745_00732" }, { "type": "cds", "sequence": "contig_1", "start": 416243, "stop": 416452, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAAAACAAAGGCGCAAACCCAGTATTTAACTGAAGAACAAATGATCTTGGGTCCGCATTTGGTTAACTTTACTGATAAACAAATGTTAGTGAAATACTACAACGGCTTGCTAAAAAACTTTACAGCACGGTTAAATCCAAGCCAGTTTGATAGTCAAGTAATCCATGTATTAAATAAAATTATTAAAGCATATGAACGCAGCTAG", "aa": "MDKTKAQTQYLTEEQMILGPHLVNFTDKQMLVKYYNGLLKNFTARLNPSQFDSQVIHVLNKIIKAYERS", "aa_hexdigest": "5ba0c7c6b4b3478b5eb2ca5eacda8290", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8104.299299999999, "isoelectric_point": 9.347029304504396 }, "id": "GIHJHMOALN_759", "locus": "GCA009939745_00733" }, { "type": "cds", "sequence": "contig_1", "start": 416510, "stop": 416902, "strand": "-", "frame": 3, "gene": "trmK", "product": "Putative tRNA methyltransferase MPN_351", "db_xrefs": [ "COG:COG2384", "COG:J", "EC:2.1.1.-", "GO:0005737", "GO:0008033", "GO:0032259", "GO:0160105", "KEGG:K06967", "SO:0001217", "UniRef:UniRef50_P47490", "UniRef:UniRef90_P75427" ], "nt": "ATGAAAAGAAGGATTAGCACAATTGCTAATTTAGTCTTACAGCAAAAACCCAAAGCCTTTTATGACATTGGTTGTGACCATGGCTATTTAGCACGTGAACTAGTTCAACAAAACCCTAGTTTAGTGGGCGTTAACAGTGATATTAGTGCTAATGCATTAGGTGCTGCTAAAGGGTTGTTAAAAGATCACACTAACATGCACTTTATTACCAGTGACGGGTTTGACCTGTTACCTAACTTAAACTTAGATCCTGCTGTTGGTGTTATTGCTGGTTTAGGTGGGTTGAAAATTATGCAAATACTAGCACAGCACAATAACTTTTTAAAGCACTTTGTACTCCAACCGCAGTCCAACATCATTGAATTGCGCCGCTTTTTAAGTGCTAATCAATGA", "aa": "MKRRISTIANLVLQQKPKAFYDIGCDHGYLARELVQQNPSLVGVNSDISANALGAAKGLLKDHTNMHFITSDGFDLLPNLNLDPAVGVIAGLGGLKIMQILAQHNNFLKHFVLQPQSNIIELRRFLSANQ", "aa_hexdigest": "b75d3079488211a97740c68c3bcbca6a", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmK" ], "truncated": "5-prime", "pseudogene": { "start": 416243, "stop": 416902, "observations": { "mutation": [ 416512 ], "stop": [ 416512 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75427", "query_cov": 1.0, "subject_cov": 0.593607305936073, "identity": 0.992, "score": 256.0, "evalue": 3.84e-85, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MKRRISTIANLVLQQKPKAFYDIGCGHGYLARELVQQNPSLVGVNSDISANALGAAKGLLKDHTNMHFITSDGFDLLPNLNLDPAVGVIAGLGGLKIMQILAQHNNFLKHFVLQPQSNIIELRRFLSANQWTITTETLVEERGFIYLVLAVDKTKAQTQYLTEEQMILGPHLVNFTDKQMLVKYYNGLLKNFTARLNPSQFDSQVIHVLNKIIKAYERS" }, "paralog": false, "identity": 0.9908675799086758, "score": 426.0, "evalue": 1.33e-154, "description": "Internal stop codon at 416512. Nonsense mutation around 416512.", "psc": { "uniref90_id": "UniRef90_P75427", "gene": "TrmK", "product": "Putative tRNA methyltransferase MPN_351", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47490", "cog_id": "COG2384", "cog_category": "J", "kegg_orthology_id": "K06967", "go_ids": [ "GO:0005737", "GO:0160105", "GO:0032259", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75427", "EC:2.1.1.-", "UniRef:UniRef50_P47490", "COG:COG2384", "COG:J", "KEGG:K06967", "GO:0005737", "GO:0160105", "GO:0032259", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_P47490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47490" ], "product": "Putative tRNA methyltransferase MG248" } }, "id": "GIHJHMOALN_760", "locus": "GCA009939745_00734" }, { "type": "cds", "sequence": "contig_1", "start": 416886, "stop": 418385, "strand": "-", "frame": 2, "gene": "sigA", "product": "RNA polymerase sigma factor", "db_xrefs": [ "COG:COG0568", "COG:K", "GO:0003677", "GO:0005737", "GO:0006352", "GO:0016987", "RefSeq:WP_010874708.1", "SO:0001217", "UniParc:UPI0000134830", "UniRef:UniRef100_P78022", "UniRef:UniRef50_P78022", "UniRef:UniRef90_P78022" ], "nt": "ATGTCATCGCCAAAGAAAAATTTCAAAAAACCTCAGCCTAAAACTGAAAATCAAAAACAAGCACTTAACGAAGAGCGCATTGCTGAACTTAAAAAGAGTCGTATCCTCGGTAAAAACCGTCCGTTTAAAAAGATGATTTACGTTGACACTAAGGCACAACGTAAACAAAAACACGAAAACGTAGCCTTTTTAAAAACGCTTCAAGAAAACAAGGAGAGTGATGTACCAAAGAAGCGTCGCGGTCGAAAGCCAAAACACGCACCTTTAAAGGAAAAGAACAACCTTAAGCTGTTTGATATTTTAGAAGGCTCTCTAAAGAGTCATACCGAAAATGATGATACTAACAAGGTAATTAGCCTGTTGGTGGAGGTGTGGGAGAAAAAGAACAAAAAGAAAGACAATAACTCGCTTTCCAACAAGGACATTGTTAATGTTTTGTCCAAGTTTGAACTCCCAGATGATGAAATTATCTTTGTTTTGGATGAACTGCGTGACAAGGGAATTGAACTACCACATGATGTGGAAGAGCACATCCATGAGTTCCGTGCTAACCAAGACTTGTCGATCATTGACGAGGACATTGAAGAATTAACCACCAAAAACATCTCTAATCGTGATAAGGTTGATGACAACGTACGTTTCTTTCTAGGTTCGCTCGATTCTTCCAAGATGTTGGACTTTGAGTCTGAGCAGCGAATTGCCAAAGTTTTAAACAGTACTGATGAAGAATCACGCAAGTATGCCATTAATCAACTGGTAACCTCTAACCTTAGGTTAGTGGTCTCCATTGCTAAAAAACACTTGGAAAGAGGATTGGATTTTAATGACCTTATCCAGGAAGGTAACCTAGGACTTTTAAAGGCCATTTCCAAGTTTAACTGGTCATTGGGTAATAAGTTTTCCACTTATGCTACTTGGTGGATTAAACAAGCGATTACTCGTGCTATTGCTGATCAAGCACGTACGGTTCGGATTCCCGTACACATGGTGGAAACTATTAACCGTTTAGCTAAAGCTGAACGTGCCTTAAACCAAGAATTAGGACGCGAACCCACCGCGGAAGAGTTAGCGGAAAAAATGGGTGGTCAAGCTGAAGGGTTTACAGTTAAAAAGATTGCTGAAATTAAACGTTTAAGCTTAGATCCTGTTTCTTTAGATAAAACAGTGGGCCACGATGAAGAATCGCAGTTCGGTGACTTTGTGCGTGATACTGATGCCCAAATGCCCGATGAGTTTACCGAAAGCCGTTCTAATTACGAAAAGATTGATGAGTTGTTAAACAACTGTTTGTCCGAACAGGAAGAATTAATTGTGCGGATGCGCATTGGTATGCCACCTTACAACGAAACCAAAACACTCGATGAGGTAAGTCAAAAGATTAAGATTCCCCGCGAAAAGATTCGGCAAATAGAAACCAAAGCCATTCGCAAACTGCGTCAAGCAGTGCGTAATAACCACATGAGCCTTTCCTTCATGAGGGGAAATGAAAAGAAGGATTAG", "aa": "MSSPKKNFKKPQPKTENQKQALNEERIAELKKSRILGKNRPFKKMIYVDTKAQRKQKHENVAFLKTLQENKESDVPKKRRGRKPKHAPLKEKNNLKLFDILEGSLKSHTENDDTNKVISLLVEVWEKKNKKKDNNSLSNKDIVNVLSKFELPDDEIIFVLDELRDKGIELPHDVEEHIHEFRANQDLSIIDEDIEELTTKNISNRDKVDDNVRFFLGSLDSSKMLDFESEQRIAKVLNSTDEESRKYAINQLVTSNLRLVVSIAKKHLERGLDFNDLIQEGNLGLLKAISKFNWSLGNKFSTYATWWIKQAITRAIADQARTVRIPVHMVETINRLAKAERALNQELGREPTAEELAEKMGGQAEGFTVKKIAEIKRLSLDPVSLDKTVGHDEESQFGDFVRDTDAQMPDEFTESRSNYEKIDELLNNCLSEQEELIVRMRIGMPPYNETKTLDEVSQKIKIPREKIRQIETKAIRKLRQAVRNNHMSLSFMRGNEKKD", "aa_hexdigest": "8bec5144e8d4fcd777528620d647dbbf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134830", "ncbi_nrp_id": "WP_010874708.1", "uniref100_id": "UniRef100_P78022", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134830", "RefSeq:WP_010874708.1", "UniRef:UniRef100_P78022" ] }, "ips": { "uniref100_id": "UniRef100_P78022", "uniref90_id": "UniRef90_P78022" }, "psc": { "uniref90_id": "UniRef90_P78022", "gene": "sigA", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_P78022", "cog_id": "COG0568", "cog_category": "K", "go_ids": [ "GO:0005737", "GO:0003677", "GO:0016987", "GO:0006352" ] }, "pscc": { "uniref50_id": "UniRef50_P78022", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78022" ], "product": "RNA polymerase sigma factor SigA" }, "genes": [ "sigA" ], "id": "GIHJHMOALN_761", "locus": "GCA009939745_00735" }, { "type": "cds", "sequence": "contig_1", "start": 418438, "stop": 418653, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAGCCTGATTGGGCTGTGTTTAACAGTATTTTAGGGGAACAACAAGCAATGTTTCCAACAACTTTTTTAGCGCAAATTAAGGAGTTCTTTTTAAACAAATCACTCTCATATGACCCTGAGGATTATGAGGAAGATTTACAGTTTTTTCGGCAACTAATTGTGAAACAAAAAGAGCTTTTAAAATATTTTAAAAGCATGGTTGAGCACTAA", "aa": "MQEPDWAVFNSILGEQQAMFPTTFLAQIKEFFLNKSLSYDPEDYEEDLQFFRQLIVKQKELLKYFKSMVEH", "aa_hexdigest": "86b33f35e0a08ef27e88393a2e9f8caa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8599.727399999998, "isoelectric_point": 4.637345695495605 }, "id": "GIHJHMOALN_762", "locus": "GCA009939745_00736" }, { "type": "cds", "sequence": "contig_1", "start": 418828, "stop": 419229, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTAGCTGAAGGTAGTGATGCGGTTAAAGCTAAGACTCACAGGGTTTTAAACCTCGTGGAATACTTAGTGGCTTACTTTAAAACCAAGGGCTTTGATGAGCGGATTACCGTCAATAAAGTTATCGACATTATTGCACAAAACCAGAAAGTAACAGCTGACACTAGCTTTAGCCGCTTTTTGTGTCAGAAATTACAGCAATTGTTGCAATACAGTGATGTGGAAACACTGTTCACACAATTACAGCAGCAAAAGCTCAAAGTAAAAGTTAATAAAACTACAACTTTTACCCAAAGAGCCCCGATTTACGAATCAGTGGTTGGTGTGGTTGATAATAGTTTTAGAAATGAATCGCAGCCGGTTGCCATTACCAAGGAGTTTTTAGTCGAAAACAACTGA", "aa": "MFLAEGSDAVKAKTHRVLNLVEYLVAYFKTKGFDERITVNKVIDIIAQNQKVTADTSFSRFLCQKLQQLLQYSDVETLFTQLQQQKLKVKVNKTTTFTQRAPIYESVVGVVDNSFRNESQPVAITKEFLVENN", "aa_hexdigest": "8447be9a46f79bab4d6608b95f274484", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15239.282399999996, "isoelectric_point": 9.013985633850098 }, "id": "GIHJHMOALN_763", "locus": "GCA009939745_00737" }, { "type": "cds", "sequence": "contig_1", "start": 419260, "stop": 419661, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08275.17", "PFAM:PF13662.12" ], "nt": "ATGATTCCGATCCATGACCTTAAGGGTAATCCAGTAGGTTTTTCAGGGCGTGCACTGCAAAAAACGGAAAAGATTAAATACAAAAACAGTGCAGAACACCAGTGGTTTAAGAAGAGTGAATTGTTATTTAACTTCCACCGGATTGATAAAAACACACTAAAGCTGTATTTAGTTGAGGGTTACTTCGATGTCTTTGCCCTTACTAGCGCTGGGATTGGTGATGTAGTGGGCTTAATGGGTTTAGCCTTGAGTGAAAGCCACATTATTGCCTTTCAACAGCAACTAAAAGCGCTAGAAACAGTGGTCTTGGCACTCGATAATGATACAGCGGGTCACGATGCTACTTTTAAGCTATTACAAGAGTTAAACGCACACGGCATTATTGTGGAAGTGGTCGATTGA", "aa": "MIPIHDLKGNPVGFSGRALQKTEKIKYKNSAEHQWFKKSELLFNFHRIDKNTLKLYLVEGYFDVFALTSAGIGDVVGLMGLALSESHIIAFQQQLKALETVVLALDNDTAGHDATFKLLQELNAHGIIVEVVD", "aa_hexdigest": "52237bdf68ef7cdbe2c613521183cc7f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08275.17", "name": "DNAG_N", "length": 47, "aa_cov": 0.3533834586466165, "hmm_cov": 1.0, "evalue": 1.1937251748437458e-17, "score": 59.711673736572266, "start": 1, "stop": 47 }, { "id": "PF13662.12", "name": "Toprim_4", "length": 82, "aa_cov": 0.5864661654135338, "hmm_cov": 1.0, "evalue": 4.417346807872776e-16, "score": 54.42559051513672, "start": 55, "stop": 132 } ], "seq_stats": { "molecular_weight": 14807.884399999984, "isoelectric_point": 6.041268730163575 }, "id": "GIHJHMOALN_764", "locus": "GCA009939745_00738" }, { "type": "cds", "sequence": "contig_1", "start": 420061, "stop": 420300, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01807.25" ], "nt": "ATGACCAGTCCCACTAGCCTAGATCAGCTCAAACAGCAAATCAAAATAGCTCCCATAGTGGAGCACTATGCCATTAAGCTAAAAAAGAAGGGGAAGGACTTTGTGGCACTCTGTCCCTTTCATGCTGACCAAAACCCCTCGATGACGGTGTCAGTTGCAAAGAACATCTTTAAGTGTTTCTCGTGTCAGGTGGGCGGTAATGGGATTGCTTTTATCCAAAAAATTGATCAGGTAGATTGA", "aa": "MTSPTSLDQLKQQIKIAPIVEHYAIKLKKKGKDFVALCPFHADQNPSMTVSVAKNIFKCFSCQVGGNGIAFIQKIDQVD", "aa_hexdigest": "892d3f54cfc6476dc5b8e05308af2bc7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01807.25", "name": "Zn_ribbon_DnaG", "length": 76, "aa_cov": 0.9620253164556962, "hmm_cov": 0.9868421052631579, "evalue": 2.6129126265005945e-32, "score": 106.07622528076172, "start": 4, "stop": 79 } ], "seq_stats": { "molecular_weight": 8723.151799999998, "isoelectric_point": 9.125064659118653 }, "id": "GIHJHMOALN_765", "locus": "GCA009939745_00739" }, { "type": "cds", "sequence": "contig_1", "start": 420491, "stop": 421222, "strand": "-", "frame": 3, "gene": "glyS", "product": "glycine--tRNA ligase", "db_xrefs": [ "COG:COG0423", "COG:J", "EC:6.1.1.14", "GO:0000166", "GO:0004820", "GO:0005524", "GO:0005737", "GO:0006426", "GO:0046983", "KEGG:K01880", "SO:0001217", "UniRef:UniRef50_P75425", "UniRef:UniRef90_P75425" ], "nt": "TTGTTCCAAACTGAAATTGGCGTTGTCAACAGCGAAAAACGCCTGGTTTACCTTCGTCCGGAAACAGCTCAGGGCATCTTCATTAACTTTAAACAGCTGTTACAACTCAAAAAGCGTCCTTTACCGTTTGGCGTAGCCCAGTTTGGTAAGAGCTTTCGCAATGAAGTGACCCCGGGAAACTTTTTGTTTCGCGTTCGTGAATTTGAACAGTTTGAAATGGAGTGGTTCTGCAATCCTCAAGCCAGTTTAAGCGTTTTTGAAAGCCAACAACAAGCAATTGCTCACTTTTTGTTTAAGGTACTGCAATTAAACCCAGCATTGGTTAAACAATACGAGTATGACAAGAATGAATTAGCACACTATGCCAATAAAACAGTGGATTTTTTATTCCAGTTTCCCCATGGCTTGAGGGAATTGTGGGGTTTAGCTGATCGTGGTACCTTTGATTTAGAACAACACCAAAAGTACGCCAAAAAGCCCTTAGACTTCTTTGATGGAGAGAATAACGAACACTTTATTCCAGCTGTAGTGGAGCCTTCGGTGGGAATTGAGCGCTTGTTTTATGCCTTGATTGTTAGCAGCTATCAACAAGAGCAGTTGGAAGGGGAAATGCGCGAAGTGTTACGTTTACCCTTTCACTTATGTCCAGAACAAATTGTAGTGCTGCCCTTAGTCAATAAACTCAAAGAAACAGCACAAACATTATTTGAAGCGCTGAGTCAAACCCACTGA", "aa": "MFQTEIGVVNSEKRLVYLRPETAQGIFINFKQLLQLKKRPLPFGVAQFGKSFRNEVTPGNFLFRVREFEQFEMEWFCNPQASLSVFESQQQAIAHFLFKVLQLNPALVKQYEYDKNELAHYANKTVDFLFQFPHGLRELWGLADRGTFDLEQHQKYAKKPLDFFDGENNEHFIPAVVEPSVGIERLFYALIVSSYQQEQLEGEMREVLRLPFHLCPEQIVVLPLVNKLKETAQTLFEALSQTH", "aa_hexdigest": "f9147e9b9cf98d319e9b7c8c23760b72", "start_type": "TTG", "rbs_motif": null, "genes": [ "glyS" ], "truncated": "both", "pseudogene": { "start": 420284, "stop": 421522, "observations": { "mutation": [ 420493, 421294, 421408 ], "stop": [ 420493, 421294, 421408 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75425", "query_cov": 1.0, "subject_cov": 0.5412026726057907, "identity": 0.996, "score": 493.0, "evalue": 1.95e-173, "gene_start": 1, "gene_end": 243, "reference_start": 138, "reference_end": 380, "reference_sequence": "MAQVYSQEVYVQYLKRYGFVFQSSELYNGLANSWDFGPLGAVLKQQIKTALYNFFIKNKRDVLLIDTPIILNEQIWKASGHLANFTDALVDCKSCKLRFRVDHLDEQIKSATQWNPKQVNCPNCKANNWSEVRDFNLLFQTEIGVVNSEKRLVYLRPETAQGIFINFKQLLQLKKRPLPFGVAQFGKSFRNEVTPGNFLFRVREFEQFEMEWFCNPQASLSVFESQQQAIAHFLFKVLQLNPALVKQYEYDKNELAHYANKTVDFLFQFPHGLRELWGLADRGTFDLEQHQKYAKKPLDFFDGENNEHFIPAVVEPSVGIERLFYALIVSSYQQEQLEGEMREVLRLPFHLCPEQIVVLPLVNKLKETAQTLFEALSQTHWRIGFESAGSIGKRYRKADAIGTKFAITFDFESLEDQAVTIRERDSLKQVRVPIKELKAWFAQHDDQSH" }, "paralog": false, "identity": 0.9927184466019418, "score": 821.0, "evalue": 6.07e-305, "description": "Internal stop codon at 420493, 421294, 421408. Nonsense mutation around 420493, 421294, 421408.", "psc": { "uniref90_id": "UniRef90_P75425", "gene": "glyS", "product": "glycine--tRNA ligase", "ec_ids": [ "6.1.1.14" ], "uniref50_id": "UniRef50_P75425", "cog_id": "COG0423", "cog_category": "J", "kegg_orthology_id": "K01880", "go_ids": [ "GO:0000166", "GO:0004820", "GO:0005524", "GO:0005737", "GO:0006426", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75425", "EC:6.1.1.14", "UniRef:UniRef50_P75425", "COG:COG0423", "COG:J", "KEGG:K01880", "GO:0000166", "GO:0004820", "GO:0005524", "GO:0005737", "GO:0006426", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P75425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75425" ], "product": "Glycine--tRNA ligase" } }, "id": "GIHJHMOALN_766", "locus": "GCA009939745_00740" }, { "type": "cds", "sequence": "contig_1", "start": 421406, "stop": 421633, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCAAGTATATTCCCAAGAAGTGTATGTGCAATACCTCAAGCGCTATGGCTTTGTCTTTCAAAGTAGTGAGCTTTACAATGGACTTGCCAACAGCTGGGACTTCGGTCCGTTGGGTGCCGTTTTAAAGCAACAAATTAAAACGGCACTGTACAACTTCTTCATTAAAAATAAGCGGGATGTATTGTTGATTGACACGCCAATTATTCTCAACGAACAGATCTGA", "aa": "MAQVYSQEVYVQYLKRYGFVFQSSELYNGLANSWDFGPLGAVLKQQIKTALYNFFIKNKRDVLLIDTPIILNEQI", "aa_hexdigest": "8a04040f23482aca9578b1a785557d31", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8752.998099999997, "isoelectric_point": 7.961281394958497 }, "id": "GIHJHMOALN_767", "locus": "GCA009939745_00741" }, { "type": "cds", "sequence": "contig_1", "start": 421874, "stop": 422383, "strand": "-", "frame": 3, "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "db_xrefs": [ "COG:COG0566", "COG:J", "EC:2.1.1.-", "GO:0003723", "GO:0005829", "GO:0006396", "GO:0008173", "GO:0032259", "SO:0001217", "UniRef:UniRef50_P47494", "UniRef:UniRef90_P75424" ], "nt": "ATGAAGCGGATGCAAGAAACCAGTTTTTTATTTGGTAGTAAGGCCTTTTTGGAAGCGTTAGATAACCAACTGCAAATTAAAAAGGTCAATTTATCTGATAAGCACCAAAAGCTGTTGGGCTTAATTAAGAAACGGGGATTGCGGTATGAAATGCACTCCAGCCAGTGGTTTCACCAGCAGTTTCGCCACATTAACCACCAGGAATTTGTTTGTGTAATCAACCCTAACCAAATGCTCAAAACAATTGAGCAGTTAATCCAAATTACAGACAGCAAGTCCACCAGTACCCTGGTAATGCTCGATGAGATCCAAGATCTGCATAACTTTGGCGCCATCTTGCGCACCTGTATGGCAGCTGAAGTGGATGGGATTATCTTTAAAAAGCATAACCAGGCGCCCATTAACAGTACCGTTATTAGAACCAGTATGGGAACTGTCTTTTACCAGAACTTAGTGCAGGTTACTAACCTCAGTTATGCCATTACTACCCTGCAAAAACACGGCTTTTGA", "aa": "MKRMQETSFLFGSKAFLEALDNQLQIKKVNLSDKHQKLLGLIKKRGLRYEMHSSQWFHQQFRHINHQEFVCVINPNQMLKTIEQLIQITDSKSTSTLVMLDEIQDLHNFGAILRTCMAAEVDGIIFKKHNQAPINSTVIRTSMGTVFYQNLVQVTNLSYAITTLQKHGF", "aa_hexdigest": "1d35decbdb7125c536a9cf03cabbbcfb", "start_type": "ATG", "rbs_motif": "AAAAA", "genes": [ "rlmB" ], "truncated": "5-prime", "pseudogene": { "start": 421655, "stop": 422383, "observations": { "mutation": [ 421666, 421876 ], "stop": [ 421666, 421876 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75424", "query_cov": 1.0, "subject_cov": 0.6983471074380165, "identity": 0.988, "score": 327.0, "evalue": 2.8e-112, "gene_start": 1, "gene_end": 169, "reference_start": 1, "reference_end": 169, "reference_sequence": "MKRMQETSFLFGSKAFLEALDNQLQIKKVNLSDKHQKLLGLIKKRGLRYEMHSSQWFHQQFRHINHQEFVCVINPNQMLKTIEQLIQITDSKSTSTLVMLHEIQDPHNFGAILRTCMAAEVDGIIFKKHNQAPINSTVIRTSMGTVFYQNLVQVTNLSYAITTLQKHGFWTVATTLDERLKPKDYRQVDFDKRILLVGNEDKGLNALLLKNADLKVKIPMNPKLNSLNVSVAVGIILFGWKS" }, "paralog": false, "identity": 0.9834710743801653, "score": 465.0, "evalue": 8.11e-169, "description": "Internal stop codon at 421666, 421876. Nonsense mutation around 421666, 421876.", "psc": { "uniref90_id": "UniRef90_P75424", "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47494", "cog_id": "COG0566", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0006396", "GO:0008173", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75424", "EC:2.1.1.-", "UniRef:UniRef50_P47494", "COG:COG0566", "COG:J", "GO:0003723", "GO:0005829", "GO:0006396", "GO:0008173", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_P47494", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47494" ], "product": "Uncharacterized tRNA/rRNA methyltransferase MG252" } }, "id": "GIHJHMOALN_768", "locus": "GCA009939745_00742" }, { "type": "cds", "sequence": "contig_1", "start": 422536, "stop": 422808, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09190.17" ], "nt": "TTGGATTTAAGCCAATCTTTAATAGAGCAAGCTTGCAGTGATATTAAAAGGATCCAAAAGGCAGTCAATGTGTGCCGCACTTGGTTTGTGTATTCTGAACAATCCGCCATTCCCGCGCCCAAACAGTTTGAACCGGTGTTTAAGGCACTTCTCAATAACCTCAACTTTGCTAATGCCATTACCCACATTTGGAAGTTAGTCAAACAAATTAACCATGATGTAAGCAAGCAAAACTTGAGCGGTTTAAAGGAACATTTAAGCCATTTGGAGTGA", "aa": "MDLSQSLIEQACSDIKRIQKAVNVCRTWFVYSEQSAIPAPKQFEPVFKALLNNLNFANAITHIWKLVKQINHDVSKQNLSGLKEHLSHLE", "aa_hexdigest": "3eff6f093267ab8f71ec489b00920ee5", "start_type": "TTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09190.17", "name": "DALR_2", "length": 45, "aa_cov": 0.4444444444444444, "hmm_cov": 1.0, "evalue": 4.133310128452691e-06, "score": 22.796756744384766, "start": 48, "stop": 87 } ], "seq_stats": { "molecular_weight": 10315.780699999996, "isoelectric_point": 8.664438056945801 }, "id": "GIHJHMOALN_769", "locus": "GCA009939745_00743" }, { "type": "cds", "sequence": "contig_1", "start": 422839, "stop": 423093, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01406.26" ], "nt": "GTGGAGTGTGCCTTTTTAATTGATTACAGCTTTAAAGACCAGTTGACAATTCATGGTGGTGGGGTGGATTTGAAGTTTCCCCATCATGAAAACGAAAACGCAATGCATATGGCACTGTATGACAAACCATTAACGCAGCATTGGATGCACATTGGCCACTTAATGTTTGAAAACCAAAAGATGTCCAAGTCACTGCAAAACTTTCTTTTAGCAGTTGACTTTTTGACAATCCATGACTTTCGCATTTTGCGCTGA", "aa": "MECAFLIDYSFKDQLTIHGGGVDLKFPHHENENAMHMALYDKPLTQHWMHIGHLMFENQKMSKSLQNFLLAVDFLTIHDFRILR", "aa_hexdigest": "16460efba93f13186417598b445de777", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 84, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.314620195868986e-36, "score": 121.94621276855469, "start": 1, "stop": 84 } ], "seq_stats": { "molecular_weight": 9957.436399999991, "isoelectric_point": 6.196439170837402 }, "id": "GIHJHMOALN_770", "locus": "GCA009939745_00744" }, { "type": "cds", "sequence": "contig_1", "start": 423157, "stop": 423699, "strand": "-", "frame": 1, "gene": "cysS", "product": "cysteine--tRNA ligase", "db_xrefs": [ "COG:COG0215", "COG:J", "EC:6.1.1.16", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270", "KEGG:K01883", "SO:0001217", "UniRef:UniRef50_P47495", "UniRef:UniRef90_P75423" ], "nt": "ATGAACCAATTTGAACCTAAATTTACCCTCATTGACACGGTGAGCAATCAGAGCGTGGTATTAGAACAAAAGCAAATTAACATTTATCTGTGTGGACCGACTGTTTACAATGATTTGCACCTAGGCAATACCCGTCCCTTAATTGTGTTTGATGTGCTACAAAGGGTGTTGCAAGCAGCGCAGTACAAGGTGCAGTTTGTCCAAAACATCACTGATATTGACGACAAGATTATCAAGATCGCGCAGCAACAGGAAATTAGTGAAGCGCAGTTGTGCAAACAGCAAATCACCGCTTACAAAAGCTTGTTGAAAAAGCTCAACATTTTGCCGATTAAACACCTCCAAGTCACCGATAAAATTGACAAAATGCCCGGTTACATTGCCCGGTTAGTCCAAATGGGCTTTGCTTACGTTTCGCCTTTGGGCAACACGTACTTTAGTGTAAGCCAATTACCCCAGTATGGTATTTTAGCCAACCGTGTTGTGGAAACAATTGAGGATGAAGCTACTGACAAACGCAATAAACTGGACTTTGTTTTGTGA", "aa": "MNQFEPKFTLIDTVSNQSVVLEQKQINIYLCGPTVYNDLHLGNTRPLIVFDVLQRVLQAAQYKVQFVQNITDIDDKIIKIAQQQEISEAQLCKQQITAYKSLLKKLNILPIKHLQVTDKIDKMPGYIARLVQMGFAYVSPLGNTYFSVSQLPQYGILANRVVETIEDEATDKRNKLDFVL", "aa_hexdigest": "2aed8abd60af96646db94d4d2e6e8bb8", "start_type": "ATG", "rbs_motif": null, "genes": [ "cysS" ], "truncated": "5-prime", "pseudogene": { "start": 422854, "stop": 423699, "observations": { "mutation": [ 423099, 423120, 423132, 423159 ], "stop": [ 423099, 423120, 423132, 423159 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75423", "query_cov": 1.0, "subject_cov": 0.41189931350114417, "identity": 0.9890000000000001, "score": 347.0, "evalue": 5.14e-117, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MNQFEPKFTLIDTVSNQSVVLEQKQINIYLCGPTVYNDLHLGNTRPLIVFDVLQRVLQAAQYKVQFVQNITDIDDKIIKIAQQQEISEAQLCKQQITAYKSLLKKLNILPIKHLQVTDKIDKMPGYIARLVKKGFAYVSPLGNTYFSVSQLPQYGILANRVVETIEDEATDKRNKLDFVLWKQTTAGVKWNSPWGWGRPGWHVECAFLIDYSFKDQLTIHGGGVDLKFPHHENENAMHMALYDKPLTQHWMHIGHLMFENQKMSKSLQNFLLAVDFLTIHDFRILRWLFYQKHYYHPLDLSQSLIEQACSDIKRIQKAVNVCRTWFVYSEQSAIPAPKQFEPVFKALLNNLNFANAITHIWKLVKQINHDVSKQNLSGLKEHLSHLEWALNILGIGFKSIHTKLNVQLIKKWASLRKNGQLDKADEVRQKLIKKGLL" }, "paralog": false, "identity": 0.9786476868327402, "score": 551.0, "evalue": 8.38e-200, "description": "Internal stop codon at 423099, 423120, 423132, 423159. Nonsense mutation around 423099, 423120, 423132, 423159.", "psc": { "uniref90_id": "UniRef90_P75423", "gene": "cysS", "product": "cysteine--tRNA ligase", "ec_ids": [ "6.1.1.16" ], "uniref50_id": "UniRef50_P47495", "cog_id": "COG0215", "cog_category": "J", "kegg_orthology_id": "K01883", "go_ids": [ "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75423", "EC:6.1.1.16", "UniRef:UniRef50_P47495", "COG:COG0215", "COG:J", "KEGG:K01883", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006423", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47495", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47495" ], "product": "Cysteine--tRNA ligase" } }, "id": "GIHJHMOALN_771", "locus": "GCA009939745_00745" }, { "type": "cds", "sequence": "contig_1", "start": 423686, "stop": 424933, "strand": "-", "frame": 3, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "COG:COG0272", "COG:L", "EC:6.5.1.2", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47496", "UniRef:UniRef90_P78021" ], "nt": "GTGTCCAAAGCGGTGGCTAGCTTTGATCGACCCGAAGCATTAATTGAGTACCTCGATCAGTTTGACCAAACACGTGATCAGTTGAACTTCCAAGTCGATGGCCTAGTGGTTAAACTAGATAACTTTCACTTTTACGACCAGTTAGGGTTTACTAGTAAGTTTCCCCGTTGGGCGTTGGCGTACAAGTTTAAACCGAAGTTTGTCCAAACCAAACTGCGTGGTGTCTTGGTAACAGTGGGACGCACTGGCAAGATTAACTATACCGCGCAGTTAGATCCAGTTAACTTAGAGGGGACAGTAGTATCAGCAGCCACACTGCATAACTTTGACTACATTAAGGAGAAGGACATTCGTTTAAACGATACAGTGATAATTTACAAAGCGGGGGAAATTATCCCTAAGGTATTGGAGGTGTGTTTACCGTTAAGAACAGCAGCGAATCAACCGATTCCCGAACAAACCCACTGTCCGGCATGTCAAGCCCCGTTAGTGCAGTATAAGGATGAGGTAGATCAGTACTGTACCAATGAACGTTGTGACCAGCGTAACCTTGAAGCGATTAATTACTTTGTGTCCAAAACGGCTATGGACATCCAGGGGCTAAGTATCCAGACCATTAGTAAGCTGTATGAAAATAACTTAGTGCGCAGCGTCGTTGATTTATACCACTTAAAGAAGCACAAAGCCGCGGTGTTAGCCTTGGAACTTAAAATTGGTGAAGTGCTGTTCAATAAACTAATCAGCAGCATCGAACAGTCCAAAACCAAGGGGATGGCACGCTTGTTAACTGGGCTTGGCATTAAACATGTGGGGCAAGTGTTAGCCAAAAGCTTAACGAAGCACTTTGAAAACATCGATGCCTTACAATCCGCCAGTATGGAAACACTGTTAGAACTCCCTGATGTGGGACCGACTGTGGCAGAATCGTTGTTTAACTGGTTTCACAATGACAATAACTTACAGCTGTTAGCTGCACTTAAAGCAGTTGGAGTGCAAATGGATGCTTTAAAGAGCAACACTAACTTTGACACCGCGAGCATTTACTTTCAAAAGAGTTTTGTTATTACTGGCAGCTTTCCAATTAGTCGCGATACCATTAAAAACTTGCTCGCCAATAAGTATGATTGTCGTTTTACAAATAACGTCACTAGTAAAGTTGATTTTGTGTTAGCTGGCATTAAGGCTACACCTCGTAAACTTGAACAAGCTAAAGCCCTCAATATTCCCATTATTAATGAACCAATTTGA", "aa": "MSKAVASFDRPEALIEYLDQFDQTRDQLNFQVDGLVVKLDNFHFYDQLGFTSKFPRWALAYKFKPKFVQTKLRGVLVTVGRTGKINYTAQLDPVNLEGTVVSAATLHNFDYIKEKDIRLNDTVIIYKAGEIIPKVLEVCLPLRTAANQPIPEQTHCPACQAPLVQYKDEVDQYCTNERCDQRNLEAINYFVSKTAMDIQGLSIQTISKLYENNLVRSVVDLYHLKKHKAAVLALELKIGEVLFNKLISSIEQSKTKGMARLLTGLGIKHVGQVLAKSLTKHFENIDALQSASMETLLELPDVGPTVAESLFNWFHNDNNLQLLAALKAVGVQMDALKSNTNFDTASIYFQKSFVITGSFPISRDTIKNLLANKYDCRFTNNVTSKVDFVLAGIKATPRKLEQAKALNIPIINEPI", "aa_hexdigest": "44c1f3348600c1bce90b74d42b4a2b7c", "start_type": "GTG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "both", "pseudogene": { "start": 423680, "stop": 425233, "observations": { "mutation": [ 423688, 424945 ], "stop": [ 423688, 424945 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78021", "query_cov": 1.0, "subject_cov": 0.6306990881458967, "identity": 0.99, "score": 700.0, "evalue": 6.8e-249, "gene_start": 1, "gene_end": 415, "reference_start": 242, "reference_end": 656, "reference_sequence": "MAKVAQIRAIVEQLKRYDYHYYVLDDPLVSDFEYDQLYKQLQALEQAHPELIQPDSPTQRVGGIVVEAFNKFPHQNPMLSLDNAFDTATIASFIANINNLTGVQNRFVVEPKIDGVSISLTYENGLLTRALTRGDGLVGEDVLSNVKTIKSIPLTIPFHKQVEIRGEIFVDKKTFAQINENLAKPFANARNLAAGTLRNLNSEVSAKRKLKAFFYFVPNALTLGCRSQSAVLAQLKQWNFPVSKAVASFDRPEALIEYLEQFDQTRDQLNFQVDGLVVKLDNFHFYDQLGFTSKFPRWALAYKFKPKFVQTKLRGVLVTVGRTGKINYTAQLDPVNLEGTVVSAATLHNFDYIKEKDIRLNDTVIIYKAGEIIPKVLEVCLPLRTAANQPIPEQTHCPACQAPLVQYKDEVDQYCTNERCDQRNLEAINYFVSKTAMDIQGLSIQTISKLYENNLVRSVVDLYHLKEHKAAVLALELKIGEVLFNKLISSIEQSKTKGMARLLTGLGIKHVGQVLAKSLTKHFENIDALQSASMETLLELPDVGPTVAESLFNWFHNDNNLQLLAALKAVGVQMDALKSNTNFDTASIYFQKSFVITGSFPISRDTIKNLLVNKYDCRFTNNVTSKVDFVLAGIKATPRKLEQAKALNIPIINEPIWT" }, "paralog": false, "identity": 0.9903288201160542, "score": 896.0, "evalue": 0.0, "description": "Internal stop codon at 423688, 424945. Nonsense mutation around 423688, 424945.", "psc": { "uniref90_id": "UniRef90_P78021", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_P47496", "cog_id": "COG0272", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78021", "EC:6.5.1.2", "UniRef:UniRef50_P47496", "COG:COG0272", "COG:L", "GO:0003677", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47496", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47496" ], "product": "DNA ligase" } }, "id": "GIHJHMOALN_772", "locus": "GCA009939745_00746" }, { "type": "cds", "sequence": "contig_1", "start": 424943, "stop": 425656, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23", "PFAM:PF22745.1" ], "nt": "ATGGCAAAGGTCGCACAAATTCGAGCAATAGTTGAACAGTTAAAACGCTATGATTACCATTACTATGTTTTGGACGATCCGCTGGTCAGTGACTTTGAATACGACCAGTTATACAAACAGCTGCAAGCGTTGGAGCAAGCCCATCCTGAGTTAATCCAACCCGATTCACCAACCCAACGTGTTGGGGGAATTGTAGTGGAAGCATTTAATAAGTTTCCTCACCAAAATCCAATGCTTTCCCTAGACAATGCCTTCGACACAGCAACAATTGCTAGTTTTATTGCCAACATTAACAACTTAACTGGAGTGCAAAACCGCTTTGTGGTGGAACCCAAAATTGATGGTGTAAGCATCTCTTTAACTTACGAAAACGGATTGTTAACTCGGGCTTTAACCCGCGGTGATGGACTTGTGGGTGAGGATGTTTTGAGTAATGTTAAAACGATTAAGAGCATTCCCCTAACGATTCCTTTTCACAAACAGGTGGAAATCCGCGGTGAGATCTTTGTCGATAAAAAAACATTTGCGCAAATTAACGAGAATTTAGCAAAACCGTTTGCCAATGCCCGCAATTTAGCAGCAGGTACACTACGGAACCTCAACAGTGAGGTGAGTGCCAAGCGCAAACTTAAGGCCTTCTTTTACTTTGTTCCCAACGCCTTAACTTTGGGTTGTAGGAGTCAAAGTGCCGTGTTAGCACAGTTAAAGCAGTGA", "aa": "MAKVAQIRAIVEQLKRYDYHYYVLDDPLVSDFEYDQLYKQLQALEQAHPELIQPDSPTQRVGGIVVEAFNKFPHQNPMLSLDNAFDTATIASFIANINNLTGVQNRFVVEPKIDGVSISLTYENGLLTRALTRGDGLVGEDVLSNVKTIKSIPLTIPFHKQVEIRGEIFVDKKTFAQINENLAKPFANARNLAAGTLRNLNSEVSAKRKLKAFFYFVPNALTLGCRSQSAVLAQLKQ", "aa_hexdigest": "fa3048915e09a50043b520f9e07b07fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 181, "aa_cov": 0.7172995780590717, "hmm_cov": 1.0, "evalue": 4.595850554622437e-68, "score": 224.92138671875, "start": 68, "stop": 237 }, { "id": "PF22745.1", "name": "Nlig-Ia", "length": 37, "aa_cov": 0.15611814345991562, "hmm_cov": 1.0, "evalue": 1.7897750506164144e-08, "score": 30.469568252563477, "start": 17, "stop": 53 } ], "seq_stats": { "molecular_weight": 26510.046300000016, "isoelectric_point": 8.543688774108887 }, "id": "GIHJHMOALN_773", "locus": "GCA009939745_00747" }, { "type": "tRNA", "sequence": "contig_1", "start": 425720, "stop": 425793, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 78.9, "nt": "GCAGATATAGTTCAATGGCAGAACATAACCTTGCCAAGGTTAAGATGCGGGTTCGATTCCCGTTATCTGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 425759, 425761 ], "id": "GIHJHMOALN_32", "locus": "GCA009939745_00748" }, { "type": "cds", "sequence": "contig_1", "start": 425824, "stop": 426114, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B2F78A2", "UniRef:UniRef90_UPI000B2F78A2" ], "nt": "ATGGAAACGCAAAACCAAATAGAAACGTTGCGCTATATTTTTAATCAGCTCAACAACCAAGACAAACCACAAATAATTTGGTTTTCCGGTGAAGGTGAGGATGAAAAAATTAACTTTTTAATCCGTTTGGACAATTATTTCCAACCTACCTTTGTCCAAGACCTTACTATTAACTTTCTCCCCGCTTTTGTTAAGCGAAACAAGAAAAATCCACCCAATACTTTAGCTAAGGGTAACTTTGTCAACATAGCTAATAAGTTATTGGCAGTGTTAGCGCGTTCCTTGAGCTGA", "aa": "METQNQIETLRYIFNQLNNQDKPQIIWFSGEGEDEKINFLIRLDNYFQPTFVQDLTINFLPAFVKRNKKNPPNTLAKGNFVNIANKLLAVLARSLS", "aa_hexdigest": "5b337a5ef0e9e2ad55eaf229c825958c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 425824, "stop": 426415, "observations": { "insertion": [ 426391 ], "mutation": [ 426112, 426262 ], "stop": [ 426112, 426262 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000B2F78A2", "query_cov": 1.0, "subject_cov": 0.48484848484848486, "identity": 1.0, "score": 192.0, "evalue": 8.67e-61, "gene_start": 1, "gene_end": 96, "reference_start": 1, "reference_end": 96, "reference_sequence": "METQNQIETLRYIFNQLNNQDKPQIIWFSGEGEDEKINFLIRLDNYFQPTFVQDLTINFLPAFVKRNKKNPPNTLAKGNFVNIANKLLAVLARSLSWKQLNKPQQKWLLWLLVPFLLLRQLWLKKKVSKIFQFVNERGILSFIKEQWPILTTLVTVGTTLGTPIFSITISQQKAILENAGHGAFVFLVIFRCLQLRWV" }, "paralog": false, "identity": 0.9897959183673469, "score": 379.0, "evalue": 1.29e-136, "description": "Internal stop codon at 426112, 426262. Frameshift due to insertion around 426391. Nonsense mutation around 426112, 426262.", "psc": { "uniref90_id": "UniRef90_UPI000B2F78A2", "uniref50_id": "UniRef50_UPI000B2F78A2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000B2F78A2", "UniRef:UniRef50_UPI000B2F78A2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000B2F78A2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B2F78A2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11172.653899999981, "isoelectric_point": 9.098374748229983 }, "id": "GIHJHMOALN_774", "locus": "GCA009939745_00749" }, { "type": "cds", "sequence": "contig_1", "start": 426289, "stop": 426981, "strand": "+", "frame": 1, "gene": null, "product": "putative MG255-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75422", "UniRef:UniRef90_P75422" ], "nt": "GTGGGTACTACCTTAGGTACACCCATCTTTTCGATTACCATTTCCCAGCAAAAAGCAATTTTGGAAAACGCTGGTCATGGTGCGTTTGTCTTTTTAGTAATCTTTTCGGTGTTTGCAATTGCGCTGGGTTTAGTCTCATCGTTAATCTTTTTAGTCTCATCGTTATTTTCAATTCGTCAAAAAAAGAGCTTACAACAGCTCCACCAAATTCTCTCTCGGTTAATTAACAAGTACTTCTGTTTGGCTAACAGTGAACAAAATCAAACCGGGCGGTACCAACTGAAAAATACCGGGGTGTGCTTCTTTTATGGCTTTGACTTTGAGGAAAAGGAATACATAACCCAAGCGATGAACCTACTGTTGCTCTTGAAGCAAACGAACTGCTTTGTGTTAGTCGGTTGTAAAGAGAGCAACATGTTGTTAATTAAAAACAAGGTGGAACCCGACATTAACCTTAAACAAAGTAGTCTGTACCTTGATTTAAAGAGTCAAATTAGTCCGTTGGCACAAATTAGTAAGTACAACCTCTTGTTTGAAGAGTTGGCCCTGGATGCTGACATGTTTTACTTAGAGGACTTCTTCGCCTTGTTAAAGACTCCGCGGCAAATTGTCAACTTTTTGTTTCGTATAAAACAAAACTTAAAGGAGTTTCACCAACCCCAAACGTTGTGGTTTGACTATTTGGCCTTGTGA", "aa": "MGTTLGTPIFSITISQQKAILENAGHGAFVFLVIFSVFAIALGLVSSLIFLVSSLFSIRQKKSLQQLHQILSRLINKYFCLANSEQNQTGRYQLKNTGVCFFYGFDFEEKEYITQAMNLLLLLKQTNCFVLVGCKESNMLLIKNKVEPDINLKQSSLYLDLKSQISPLAQISKYNLLFEELALDADMFYLEDFFALLKTPRQIVNFLFRIKQNLKEFHQPQTLWFDYLAL", "aa_hexdigest": "1580ff4d72680da9c7e4ae0feb568e8c", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 425989, "stop": 427284, "observations": { "mutation": [ 426112, 426262, 426979 ], "stop": [ 426112, 426262, 426979 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75422", "query_cov": 1.0, "subject_cov": 0.4307116104868914, "identity": 0.991, "score": 434.0, "evalue": 4.82e-149, "gene_start": 1, "gene_end": 230, "reference_start": 156, "reference_end": 385, "reference_sequence": "METQNQIETLRYIFNQLNNQDKPQIIWFSGEGEDEKINFLIRLDNYFQPTFVQDLTINFLPAFVKRNKKNPPNTLAKGNFVNIANKLLAVLARSLSWKQLNKPQQKWLLWLLVPFLLLRQLWLKKKVSKIFQFVNERGILSFIKEQWPILTTLVTVGTTLGTPIFSITISQQKAILENAGHGAFVFLVIFSVFAIALGLVSSLIFLVSSLFSIRQKKSLQQLHQILSRLINKYFCFANSEQNQTGRYQLKNTGVCFFYGFDFEEKEYITQAMNLLLLLKQTNCFVLVGCKESNMLLIKNKVEPDINLKQSSLYLDLKSQISPLAQISKYNLLFEELALDADMFYLEDFFALLKTPRQIVNFLFRIKQNLKEFHQPQTLWFDYLALWALVIATDFEFNNVLWSFNDYLSLTNKQKEDYASVNLTAFFNRSLKNHKDNSLLFKPELFNTHAYIPETYTQVTLENIDSDKRAQLVPLNWFSQQKFSDFIEEKINFWQTQQAENKVFYLTLGERIFFLVLVNKKFKQIKLEAALKYLN" }, "paralog": false, "identity": 0.988399071925754, "score": 836.0, "evalue": 1e-309, "description": "Internal stop codon at 426112, 426262, 426979. Nonsense mutation around 426112, 426262, 426979.", "psc": { "uniref90_id": "UniRef90_P75422", "product": "Uncharacterized protein MG255 homolog", "uniref50_id": "UniRef50_P75422", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75422", "UniRef:UniRef50_P75422", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75422" ], "product": "Uncharacterized protein MG255 homolog" } }, "id": "GIHJHMOALN_775", "locus": "GCA009939745_00750" }, { "type": "cds", "sequence": "contig_1", "start": 427021, "stop": 427302, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGGTCATTTAACGACTATTTAAGTTTAACGAACAAGCAAAAGGAAGATTATGCATCAGTTAACCTCACCGCCTTTTTTAACCGTAGTTTAAAGAACCATAAGGACAACAGCCTGCTCTTTAAACCAGAATTGTTCAACACTCACGCTTATATTCCTGAAACTTACACTCAAGTTACCTTAGAAAACATTGATTCAGACAAACGTGCGCAACTAGTGGCATTGAACTGGTTTTCACAGCAAAAGTTTAGCGATTTTATAGAAGAGAAAATTAACTTTTGA", "aa": "MWSFNDYLSLTNKQKEDYASVNLTAFFNRSLKNHKDNSLLFKPELFNTHAYIPETYTQVTLENIDSDKRAQLVALNWFSQQKFSDFIEEKINF", "aa_hexdigest": "02b2d9beeea0f06bd61d6cf547f58b5e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11092.25799999999, "isoelectric_point": 5.575132179260252 }, "id": "GIHJHMOALN_776", "locus": "GCA009939745_00751" }, { "type": "cds", "sequence": "contig_1", "start": 427327, "stop": 427428, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTACCTCACTTTGGGCGAGCGGATCTTCTTTTTAGTATTAGTTAACAAGAAGTTCAAACAAATTAAACTAGAAGCAGCGTTGAAGTACCTCAACTAA", "aa": "MFYLTLGERIFFLVLVNKKFKQIKLEAALKYLN", "aa_hexdigest": "80607b1e5c74462264a515b250e1e760", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3992.853900000001, "isoelectric_point": 9.873542594909669 }, "id": "GIHJHMOALN_777", "locus": "GCA009939745_00752" }, { "type": "tRNA", "sequence": "contig_1", "start": 427429, "stop": 427503, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 55.1, "nt": "CTTCCCTTGGTGTAATGGATAACACGACTGAGTCCTAATCAGTAAATGGAGGTTCGATTCCTCTAGGGAAGGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 427433, 427435 ], "id": "GIHJHMOALN_10", "locus": "GCA009939745_00753" }, { "type": "tRNA", "sequence": "contig_1", "start": 427577, "stop": 427650, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 74.2, "nt": "GGGGGTGTAGTTTAGTGGCAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 427609, 427611 ], "id": "GIHJHMOALN_11", "locus": "GCA009939745_00754" }, { "type": "cds", "sequence": "contig_1", "start": 427658, "stop": 428050, "strand": "+", "frame": 2, "gene": null, "product": "putative MG256-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47498", "UniRef:UniRef90_P75421" ], "nt": "ATGCATTACAGTTACAGCTTTAAAGAATACATCGAACGGTTTGCCAAAAAGGTTAATTCGATTGACAGCGAGTACTTCGAATTTTCCTCTTACATAGAGCGCATGCGCACTGTGTTTGGTTTACTGATTGCACTGATATGCTTTAGTAACGTTCTCTGTTTTCTTTTTATTGCAACTTGGTTTAGCACAAAGGGATTTGGTCAACACTACCGGGCGTTAATATTTACCTTGTTTATTCCCTTTGTCACTTCACTTTTAGCCAACATTATCTTCATTAACCTTAATCGCGCTTTTCGCGAATACTTTAAGATCAGCAGTAAAAGCCGTTCTTTTTTGGTAATCTGTGCCTTCTCCTCATTACCGATTGTGAACATCTGGCTAATGTTGTGGTGA", "aa": "MHYSYSFKEYIERFAKKVNSIDSEYFEFSSYIERMRTVFGLLIALICFSNVLCFLFIATWFSTKGFGQHYRALIFTLFIPFVTSLLANIIFINLNRAFREYFKISSKSRSFLVICAFSSLPIVNIWLMLW", "aa_hexdigest": "5be2940fbd06b38a856224268460776e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 427658, "stop": 428353, "observations": { "mutation": [ 428048 ], "stop": [ 428048 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75421", "query_cov": 1.0, "subject_cov": 0.5038759689922481, "identity": 0.992, "score": 247.0, "evalue": 4.38e-81, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MNYSYSFKEYIERFAKKVNSIDSEYFEFSSYIERMRTVFGLLIALICFSNVLCFLFIATWFSTKGFGQHYRALIFTLFIPFVTSLLANIIFINLNRAFREYFKISSKSRSFLVICAFSSLPIVNIWLMLWWVAMIKRFTSNYAFAIFDKYNGLTSGVFIFDFADNVNFEGKLVSFDNTKDTNRDFVHFYSEAKLKRDKITLQTNPIPHERMYVNRMYYQQKLSMGANQNSPSTAFANLKRYVEHKQQKIIKIKQFILT" }, "paralog": false, "identity": 0.9913419913419913, "score": 463.0, "evalue": 1.87e-168, "description": "Internal stop codon at 428048. Nonsense mutation around 428048.", "psc": { "uniref90_id": "UniRef90_P75421", "product": "Uncharacterized protein MG256 homolog", "uniref50_id": "UniRef50_P47498", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75421", "UniRef:UniRef50_P47498", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47498", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47498" ], "product": "Uncharacterized protein MG256" } }, "id": "GIHJHMOALN_778", "locus": "GCA009939745_00755" }, { "type": "cds", "sequence": "contig_1", "start": 428057, "stop": 428434, "strand": "+", "frame": 2, "gene": null, "product": "putative MG256-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47498", "UniRef:UniRef90_P75421" ], "nt": "ATGATTAAGCGCTTTACTAGTAACTATGCCTTTGCTATCTTTGATAAGTACAACGGTTTGACTAGTGGTGTGTTTATCTTTGACTTTGCTGATAATGTCAATTTTGAGGGTAAACTAGTGAGCTTTGACAATACCAAGGACACCAACCGTGACTTTGTCCACTTTTACAGTGAGGCCAAACTTAAACGCGATAAAATTACGCTACAAACGAATCCAATTCCACACGAAAGGATGTATGTTAACCGCATGTACTATCAGCAAAAGCTCAGTATGGGTGCTAACCAAAACAGCCCTTCAACGGCATTTGCTAACTTAAAGCGGTATGTGGAACATAAACAGCAAAAAATTATCAAGATTAAGCAGTTTATCTTGACCTAA", "aa": "MIKRFTSNYAFAIFDKYNGLTSGVFIFDFADNVNFEGKLVSFDNTKDTNRDFVHFYSEAKLKRDKITLQTNPIPHERMYVNRMYYQQKLSMGANQNSPSTAFANLKRYVEHKQQKIIKIKQFILT", "aa_hexdigest": "96c3cce8e61a487fbe8823ac25ab6f0f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 427757, "stop": 428434, "observations": { "mutation": [ 428048 ], "stop": [ 428048 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75421", "query_cov": 1.0, "subject_cov": 0.4844961240310077, "identity": 1.0, "score": 247.0, "evalue": 5.05e-81, "gene_start": 1, "gene_end": 125, "reference_start": 134, "reference_end": 258, "reference_sequence": "MNYSYSFKEYIERFAKKVNSIDSEYFEFSSYIERMRTVFGLLIALICFSNVLCFLFIATWFSTKGFGQHYRALIFTLFIPFVTSLLANIIFINLNRAFREYFKISSKSRSFLVICAFSSLPIVNIWLMLWWVAMIKRFTSNYAFAIFDKYNGLTSGVFIFDFADNVNFEGKLVSFDNTKDTNRDFVHFYSEAKLKRDKITLQTNPIPHERMYVNRMYYQQKLSMGANQNSPSTAFANLKRYVEHKQQKIIKIKQFILT" }, "paralog": false, "identity": 0.9955555555555555, "score": 450.0, "evalue": 1.63e-163, "description": "Internal stop codon at 428048. Nonsense mutation around 428048.", "psc": { "uniref90_id": "UniRef90_P75421", "product": "Uncharacterized protein MG256 homolog", "uniref50_id": "UniRef50_P47498", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75421", "UniRef:UniRef50_P47498", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47498", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47498" ], "product": "Uncharacterized protein MG256" } }, "id": "GIHJHMOALN_779", "locus": "GCA009939745_00756" }, { "type": "cds", "sequence": "contig_1", "start": 428479, "stop": 428772, "strand": "+", "frame": 1, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_010874716.1", "SO:0001217", "UniParc:UPI0000133F18", "UniRef:UniRef100_P78020", "UniRef:UniRef50_P47499", "UniRef:UniRef90_P78020" ], "nt": "ATGAAAAAAGATTTTCATTTTCCAAGCCAATCCGTTAGCTTCAAGTGTGCTTCTTGCAGCAATAGTTTCACCATTGAATCAACCCTAAAACAAAAAGAAATTACGATTGATATTTGTGGTAAATGTCACCCGTTTTACATTGGTGAATTAACCAAACAAACAGTCCACGGTCGTGCGGAAAAGCTTTCTGGAAAGTTCAATGCTGGCAAAGCCTTTTTAGAGAATAAAACTCCTAAAAAAGCGAAAGGCAAAACAGAAGAATATACCAAACACCGTTCTTTAAACGAACTGTAG", "aa": "MKKDFHFPSQSVSFKCASCSNSFTIESTLKQKEITIDICGKCHPFYIGELTKQTVHGRAEKLSGKFNAGKAFLENKTPKKAKGKTEEYTKHRSLNEL", "aa_hexdigest": "3c44c7a45b1bd5b7f6d5e80e4c2c5463", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133F18", "ncbi_nrp_id": "WP_010874716.1", "uniref100_id": "UniRef100_P78020", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133F18", "RefSeq:WP_010874716.1", "UniRef:UniRef100_P78020" ] }, "ips": { "uniref100_id": "UniRef100_P78020", "uniref90_id": "UniRef90_P78020" }, "psc": { "uniref90_id": "UniRef90_P78020", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_P47499", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47499" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "GIHJHMOALN_780", "locus": "GCA009939745_00757" }, { "type": "cds", "sequence": "contig_1", "start": 428773, "stop": 429213, "strand": "+", "frame": 1, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_P47500", "UniRef:UniRef90_P75420" ], "nt": "GTGGAGTTTGACAAGCAGTTCTTTTCTAGTGTTGAAAAGATTGTTGAACTTGCTGAACAACTCGAAAAGGACTTAAATAAACCCGATTTGACCTTTGAACAGATCAAAGCGATCAACAAGGAACTGAAACATAAACAACCCTTAGTTGTTAAATTCAAAGAATTTAAACGGCTCATAGATCAAGCTTTGGAAGCGGAGGCCATTTTGGAAAACAACGAGCTCAAGGAGCTACATGATGAGGCCAAAAAAGAGCTGGAACGGGTGCGCAGTGTTGTCCCTGAATACGAAGAAGCGTTAAAACTGTTGTTATTACCAATTGATGAGAATAACCAGAAAAACGTGATTGTAGAGCTTCGTCCCGCTGCTGGTGGGGATGAATCGTGCATCTTTTTAGCTGATTTATTCAATATGTACCGCAACTTTTGTACTAACAAGGGTTGA", "aa": "MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLIDQALEAEAILENNELKELHDEAKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRPAAGGDESCIFLADLFNMYRNFCTNKG", "aa_hexdigest": "c0c64d44ddf7ae8abd90fcd93b1ef3eb", "start_type": "GTG", "rbs_motif": null, "genes": [ "prfA" ], "truncated": "3-prime", "pseudogene": { "start": 428773, "stop": 429516, "observations": { "mutation": [ 429211 ], "stop": [ 429211 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75420", "query_cov": 1.0, "subject_cov": 0.40668523676880225, "identity": 0.993, "score": 272.0, "evalue": 2.81e-89, "gene_start": 1, "gene_end": 146, "reference_start": 1, "reference_end": 146, "reference_sequence": "MEFDKQFFSSVEKIVELAEQLEKDLNKPDLTFEQIKAINKELKHKQPLVVKFKEFKRLIDQALEAEAILENNELKELHDEAKKELERVRSVVPEYEEALKLLLLPIDENNQKNVIVELRPAAGGDESCIFLADLFNMYRNFCSNKGWKLQINEMIPSSVGLNFVSFEVNGVDVFAKLKFESGVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNRTESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGSGDRAEKIRTYNYPQNRVTDHRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS" }, "paralog": false, "identity": 0.9878542510121457, "score": 475.0, "evalue": 1.78e-171, "description": "Internal stop codon at 429211. Nonsense mutation around 429211.", "psc": { "uniref90_id": "UniRef90_P75420", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_P47500", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75420", "UniRef:UniRef50_P47500", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_P47500", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47500" ], "product": "Peptide chain release factor 1" } }, "id": "GIHJHMOALN_781", "locus": "GCA009939745_00758" }, { "type": "cds", "sequence": "contig_1", "start": 429232, "stop": 429852, "strand": "+", "frame": 1, "gene": null, "product": "Peptide chain release factor 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1W9TI19" ], "nt": "ATGATCCCATCCAGTGTCGGGTTAAACTTCGTTTCCTTTGAAGTTAATGGTGTCGATGTGTTTGCCAAGCTCAAGTTTGAATCTGGCGTACACCGGGTACAACGCGTACCCGCTACTGAATCCAAGGGGAGAGTACACACTTCAACCGTTACGGTTGCTGTTTTACCACAACTAGAAGCGGTTGAAGTTCATATTAACCCAGCTGATCTTAGAGTAGATACCTACCGTGCTTCGGGTGCTGGGGGTCAACACGTTAACCGGACTGAAAGTGCAGTGCGGATTACCCATTTACCAACTGGCATAGTAGTTTCGTGTCAAGAGGGTAAATCACAATTTACTAACCGTGATACGGCAATGAAGATGCTACGTGCTAAACTGTGGGAAAAAGCGCAGAACGAACAGTTATCAACCCAAGCAGGTTTGCGTAAAAGTCAGGTTGGGTCGGGTGATCGTGCCGAAAAGATCCGCACATATAACTATCCCCAAAACCGGGTCACAGACCACCGCATTAAACTTACCGTCAATAAGCTTAACACGATTATTTTAGGTGACCTTGATGAAATTATTGAAGCGTTGCAGGCAGATGAAAAGAAACAACAACTAGAGAACTTTTTCAGTTAA", "aa": "MIPSSVGLNFVSFEVNGVDVFAKLKFESGVHRVQRVPATESKGRVHTSTVTVAVLPQLEAVEVHINPADLRVDTYRASGAGGQHVNRTESAVRITHLPTGIVVSCQEGKSQFTNRDTAMKMLRAKLWEKAQNEQLSTQAGLRKSQVGSGDRAEKIRTYNYPQNRVTDHRIKLTVNKLNTIILGDLDEIIEALQADEKKQQLENFFS", "aa_hexdigest": "76d49743f1eca11ec3aa76929feb9462", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1W9TI19", "query_cov": 0.9660194174757282, "subject_cov": 0.8223140495867769, "identity": 0.643, "score": 248.0, "evalue": 1.85e-80, "valid": false, "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A0A1W9TI19", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0006415" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1W9TI19", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1W9TI19" ], "product": "Peptide chain release factor 1" }, "genes": [], "id": "GIHJHMOALN_782", "locus": "GCA009939745_00759" }, { "type": "cds", "sequence": "contig_1", "start": 429852, "stop": 429977, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTGTATGAACTTTTCTTGAACCAAAAGCTGCTGTATGGCACCGATCCGCACTTTAACGGTGTTTTTCTCACCCTATTAGAAAAGTTTGGTCTGCACTTTAAGGATTTAACAGCACTGTGA", "aa": "MNLYELFLNQKLLYGTDPHFNGVFLTLLEKFGLHFKDLTAL", "aa_hexdigest": "f75fee177eed71695c8a8abee943c0ea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4815.5853, "isoelectric_point": 5.977779579162598 }, "id": "GIHJHMOALN_783", "locus": "GCA009939745_00760" }, { "type": "cds", "sequence": "contig_1", "start": 430464, "stop": 430889, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTGATGGTAACGGAATTTTGTTTTACCAAAAGCTGTATAACTTTTTATTGGGTAACCGCCAAGTTAAACAAGTGATACTGGAGTGCTCACCAACCCAGAAAAAAGAGTTTTTAGCATTATTTAGCATTTTTAAAACTAGTGAAATTTACACAAGCCACAAGCAATTCATCGGTTTGAGTATTGACAACACTAAACTACCAGTCTTAAAAATTGCGCAAACCAAGCAAATTAAAGCACTGCTAGACAAGGGGATGACGGCCATTATCCCCACCGACACGCAAATAGGATTAATGAGTTATTGCCAACAAGATTTAGACCACATTAAACAACGCGATCCCAACAAGCACTACGTCCAGTTTTTAGCACCTAGTCAAATTAACCAGTTACCCAAACAATTACAAAAGCTAGCCAAACTGTTTTGA", "aa": "MADGNGILFYQKLYNFLLGNRQVKQVILECSPTQKKEFLALFSIFKTSEIYTSHKQFIGLSIDNTKLPVLKIAQTKQIKALLDKGMTAIIPTDTQIGLMSYCQQDLDHIKQRDPNKHYVQFLAPSQINQLPKQLQKLAKLF", "aa_hexdigest": "6a44a0a2d0cf2713a58c0cbc4ca36782", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16171.81199999998, "isoelectric_point": 9.543722343444824 }, "id": "GIHJHMOALN_784", "locus": "GCA009939745_00761" }, { "type": "cds", "sequence": "contig_1", "start": 431058, "stop": 431213, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGCAGAACTGTTTTATTGTGCAAAACAGCTTTAAAAGTAATAACGAACCGTCCTTAATTTACAACTTGGATACGAAGCAAATTGTTCGTGGTAGTTCAACCCAATTACAACGTTTCCAAGCGTTACTAGCAAAACACAAATTAAGACACTAA", "aa": "MQQNCFIVQNSFKSNNEPSLIYNLDTKQIVRGSSTQLQRFQALLAKHKLRH", "aa_hexdigest": "5629f11c7c49c8c20d46cdc6852e5a84", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5974.7895, "isoelectric_point": 10.182410621643069 }, "id": "GIHJHMOALN_785", "locus": "GCA009939745_00762" }, { "type": "cds", "sequence": "contig_1", "start": 431345, "stop": 431980, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTAAGTATGGTGCTACCCTTTTTAGTGGCTTTTTAGGGCTTTCAGCGATCCTAGCTGCTTGTGGTGCAAAGGGTAAATTTGACCAAGTTGATGATGGCAAAATTGTGTTAGCCTCTTCATTAACTTCTAAAAATGCCGCTAATGCTCTACAAGCTGTGGTGGAAAAATACAACCAGGTTAAGGGTGGCAATGATTATCCTATTGAAATTACCCAAATTACCGGTGGTTATGACGGTGGACGTGGTAACTTACAAACCAAACTTAGCGTTAAAGACAAAACGACCTTTTACAACCTCATCTTAAACTATCCCGACTTAGTTTCCACCCTAGGACGTGTTGGGATGGAACTGCCACTAGACAACGTTGATCTCTCCAAGTTATCACCTAACTTTTTGAAGTTCAACGAACGCATTAGCGGTGTAGCCAAAAAGGCCAATTACGCCATTCCCGTATCACTTTCAACTGAAGTTTTAGCTTTAAATGGACCGGTATTACACTATATCTTGAACAGCGCGAAGGGTGGTAATGAAACAGCTGTAACACAAAGAACTGCTAGCACAAACCAACAAAATACACTTCAAAATTCGTTAAAAATTAACGAAAGCGACCCCATAACCAAAGAGCTTTGA", "aa": "MKFKYGATLFSGFLGLSAILAACGAKGKFDQVDDGKIVLASSLTSKNAANALQAVVEKYNQVKGGNDYPIEITQITGGYDGGRGNLQTKLSVKDKTTFYNLILNYPDLVSTLGRVGMELPLDNVDLSKLSPNFLKFNERISGVAKKANYAIPVSLSTEVLALNGPVLHYILNSAKGGNETAVTQRTASTNQQNTLQNSLKINESDPITKEL", "aa_hexdigest": "cb19b2b7bd546923c9a5f7602f28676f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22635.497400000015, "isoelectric_point": 9.025267601013184 }, "id": "GIHJHMOALN_786", "locus": "GCA009939745_00763" }, { "type": "cds", "sequence": "contig_1", "start": 432173, "stop": 433264, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P75418" ], "nt": "GTGGAGAATGGCCTTAAGGGTTATACCTTTAAAGCTAGTGTCTTTGAAAACTGGCATGAACTGCTCGATTTTTCCGCAAGGATAGCTAGATCATTTACTAATATTCACACCAAAGAAACCAAAAAGGGAACGGACATTCAAGGTGTACTTGGGATTGACAGTACACCTAACGCCTTATTTACTTCAGTATTTGCTGCAGGTGGTGGTAACTATGACAACTTCTTTTATAAGGTGCAAGATGGTCGGGCTGACTTTAGTAACTTTAAAAATAAGGAAACCTCTTACCAAAACTTACAAAAAGTATTTAGCGATTATAAGGAGTTAATTGATAAAAACGGTTTGTTTGTCAATAAGGGTGGCTCTTACTCTTCTAACTTCCAGAAGTTCCACCAATTAGCTTACAGTATTTCTTCCACTTCAGGTTTCTTTTATTCGTTTGCTGGTAACAGTGCTAAACGCTTAAAGTTTGATGACGGTAACTTTATTGAGTTTCCGCGCTTCACTCAACCAGTTAAAGCGCCTAATAAAACAACTCAAAGTGGTGGAGAAAATAACAATAAAAGTGAAAATAATCTTCTTGGAACTTTTGAAATAGAAGACAAAAGCAAAAAGCGCGAAAGCGTTGAATCTAGTGAAAAATCAAGTTCTGGGACTCAATCCAAAAGCAAGAAAGGTAAAAGTATGAAAACCATTTACCTTTATAAAGCACAAATTCCAGAAGGAAAAAACAAAGGTGATAATGCCATCTTAATTACTAACCAAACTGTTATTGATAAACTAGAAAAAGCTGCTAAAGAAGACGGTAAAAGTGGAACTCAAGTTTCAACTGTTGCAAGTAAATCAACAACTAAAGAGAACCAAGAAAACAAAATAATTGGTTACACATCGACTGATAACCTTCGTGAAGACAATAAGAACATTTTCGATATCGATGATCTTAACGATGAAAAATATGACCGTAAGATTATTGTTAGTGCTACTGAAGAAGTATTGGAACAATCTAACACTTTACAGAGTGAAGAAGCAGTTGTTCTACCGGCACTTGCTAAATATAAAGCTAGTGATTCCACAAAAATAGCAATCACTCAATAA", "aa": "MENGLKGYTFKASVFENWHELLDFSARIARSFTNIHTKETKKGTDIQGVLGIDSTPNALFTSVFAAGGGNYDNFFYKVQDGRADFSNFKNKETSYQNLQKVFSDYKELIDKNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFDDGNFIEFPRFTQPVKAPNKTTQSGGENNNKSENNLLGTFEIEDKSKKRESVESSEKSSSGTQSKSKKGKSMKTIYLYKAQIPEGKNKGDNAILITNQTVIDKLEKAAKEDGKSGTQVSTVASKSTTKENQENKIIGYTSTDNLREDNKNIFDIDDLNDEKYDRKIIVSATEEVLEQSNTLQSEEAVVLPALAKYKASDSTKIAITQ", "aa_hexdigest": "8e7610dfec47f1672aaf2f8ae185d61d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 431873, "stop": 433567, "observations": { "mutation": [ 431978, 432155, 433262 ], "stop": [ 431978, 432155, 433262 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75418", "query_cov": 1.0, "subject_cov": 0.45775535939470363, "identity": 0.997, "score": 682.0, "evalue": 8.17e-241, "gene_start": 1, "gene_end": 363, "reference_start": 277, "reference_end": 639, "reference_sequence": "MKFKYGATLFSGFLGLSAILAACGAKGKFDQVDDGKIVLASSLTSKNAANALQAVVEKYNQVKGGNDYPIEITQITGGYDGGRGNLQTKLSVKDKTTFYNLILNYPDLVSTLGRVGMELPLDNVDLSKLSPNFLKFNERISGVAKKANYAIPVSLSTEVLALNGPVLHYILNSAKGGNETAVTQRTASTNQQNTLQNSLKINESDPITKELWEKIQKAAETNGKAKEQGKSKKQGTEKTTTLTLLKTESSQQSNTTKDGKATSDEAIKKTWGDYQKVENGLKGYTFKASVFENWHELLDFSARIARSFTNIHTKETKKGTDIQGVLGIDSTPNALFTSVFAAGGGNYDNFFYKVQDGRADFSNFKNKETSYQNLQKVFSDYKELIDKNGLFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGNSAKRLKFDDGNFIEFPRFTQPVKAPNKTTQSGGENNNKSENNLLGTFEIEDKSKKRESVESSEKSSSGTQSKSKKGKSMKTIYLYKAQIPEGKNKGDNAILITNQTVIDKLEKAAKEDGKSGTQVSTVASKSTTKENQENKIIGYTSTDNLREDNKNIFDIDDLNDEKYDRKIIVSATEEVLEQSNTLQSEEAVVLPALAKYKASDSTKIAITQEPNLIGIHANEKENEETRKFVNWFLNSEQTWESKKYSKNKQNSNEKRTPAQFFADSASYILPLKKTFDNANKKEDQSKANNKTNSQRTNTYIEKALEIFKGISQNQITSYSDPSDFRSGRFRDGIGSNFNAVVNSKVDFNKFINNFIASLGFDI" }, "paralog": false, "identity": 0.9946808510638298, "score": 1082.0, "evalue": 0.0, "description": "Internal stop codon at 431978, 432155, 433262. Nonsense mutation around 431978, 432155, 433262.", "psc": { "uniref90_id": "UniRef90_P75418", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75418", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "GIHJHMOALN_787", "locus": "GCA009939745_00764" }, { "type": "cds", "sequence": "contig_1", "start": 433764, "stop": 433943, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGAGATTTCTAGGGGCAAAGCAATTACCAAAGAATACATAAGAGCTAACCCTGGTGAATATCCTGTTTATTCTGGTTCAACATTAAATGATGGTGAAATAGGTCGCATAGATGAATGCGAATTTGATGGTGAATATGTAACTTGA", "aa": "MEIKTYKIKDICEISRGKAITKEYIRANPGEYPVYSGSTLNDGEIGRIDECEFDGEYVT", "aa_hexdigest": "6dc7da85cb8446a93bd914f3b9f7c3ac", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 61, "aa_cov": 0.9661016949152542, "hmm_cov": 0.9836065573770492, "evalue": 2.8752451363818785e-11, "score": 39.35646438598633, "start": 3, "stop": 59 } ], "seq_stats": { "molecular_weight": 6710.4442, "isoelectric_point": 4.644507408142091 }, "id": "GIHJHMOALN_788", "locus": "GCA009939745_00765" }, { "type": "cds", "sequence": "contig_1", "start": 434070, "stop": 434483, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "ATGGAAGCGCCTAAACATGTTAATAATGCTTGTGTTATTCCTAATCTAACTTTAAAAAAGATGAGAGAAATAGAACTAGATTTTCCAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGA", "aa": "MEAPKHVNNACVIPNLTLKKMREIELDFPSKKIQEKIATILDTFTELSAELRERKKQYAFYRDYLLNQENIRKIYGANIPFETFQVKDICEIRRGRAITKAYIRNNPGENPVYSAATTNDGELGRIKDCDFDGEYIT", "aa_hexdigest": "76b6e8516d96bbb35b19364825e78b37", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 62, "aa_cov": 0.4233576642335766, "hmm_cov": 0.9838709677419355, "evalue": 4.407403530403583e-21, "score": 71.28997039794922, "start": 80, "stop": 137 } ], "seq_stats": { "molecular_weight": 15910.972899999979, "isoelectric_point": 7.65106258392334 }, "id": "GIHJHMOALN_789", "locus": "GCA009939745_00766" }, { "type": "cds", "sequence": "contig_1", "start": 434869, "stop": 434991, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI001004EC6A", "UniRef:UniRef100_A0AB38W7C9", "UniRef:UniRef50_A0AB38W7C9", "UniRef:UniRef90_A0AB38W7C9" ], "nt": "ATGGGCGGCCCGCACGACATCCAGCCCCCCATTGAGCGGTGTGGTAAAGGGCGTGTAGGGGGTGAGGGTGTTGTGGTCTTGGAGGAGGTAGGTGTTGGAAACTATTTATTAAGCATTACATAA", "aa": "MGGPHDIQPPIERCGKGRVGGEGVVVLEEVGVGNYLLSIT", "aa_hexdigest": "0783f67634c017e7377b3fa28055f056", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001004EC6A", "uniref100_id": "UniRef100_A0AB38W7C9", "db_xrefs": [ "SO:0001217", "UniParc:UPI001004EC6A", "UniRef:UniRef100_A0AB38W7C9" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7C9", "uniref90_id": "UniRef90_A0AB38W7C9" }, "psc": { "uniref90_id": "UniRef90_A0AB38W7C9", "uniref50_id": "UniRef50_A0AB38W7C9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7C9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7C9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4133.705699999999, "isoelectric_point": 4.957690238952636 }, "id": "GIHJHMOALN_790", "locus": "GCA009939745_00767" }, { "type": "cds", "sequence": "contig_1", "start": 435028, "stop": 435588, "strand": "+", "frame": 1, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_P75415" ], "nt": "GTGGGACAGAACAAGTTAGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGACTCTAATAACCAAGATACGAGTCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTAGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTATACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAACAACCCCGCTCCCAAGGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGA", "aa": "MGQNKLVALVRSALVRVKFQDTTSSDSNNQDTSQNALSFDTQESQKALNGSQSGSSDTSGSNSQDLASYILIFQAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLQDLLVEQPVTPYTPNAGLARVNGVAQDTVHFGSGQESSWNSQRSQKGLKTTPLPRPSPALSSIRAARTGS", "aa_hexdigest": "2941ccc9fcf17790f3040ad278ca3ebf", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 434962, "stop": 435777, "observations": { "mutation": [ 435019, 435586 ], "stop": [ 435019, 435586 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75415", "query_cov": 1.0, "subject_cov": 0.6838235294117647, "identity": 0.9840000000000001, "score": 345.0, "evalue": 1.57e-118, "gene_start": 1, "gene_end": 186, "reference_start": 24, "reference_end": 209, "reference_sequence": "MGGLDVVRAAHLHPSYELVDWKRVGQNKLVALVRSALVRVKFQDTTSSDSNNQDTSQNALSFDTQESQKALNGSQSGSSDTSGSNSQDFASYILIFQAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLQDLLVEQPVTPYTPNAGLARVNGVAQDTVHFGSGQESSWNSQRSQKGLKTTPLPMPSPALSSIRAARTGSWMKVDRCMNPWIRPRRGRGRMRALGKIRKKQRRKMMPRWWGWLEVVRLEVLLVYKAMARTVRG" }, "paralog": false, "identity": 0.985239852398524, "score": 529.0, "evalue": 1.76e-193, "description": "Internal stop codon at 435019, 435586. Nonsense mutation around 435019, 435586.", "psc": { "uniref90_id": "UniRef90_P75415", "product": "Putative MgpC-like protein MPN_366", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75415", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "GIHJHMOALN_791", "locus": "GCA009939745_00768" }, { "type": "cds", "sequence": "contig_1", "start": 435603, "stop": 436019, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75414", "UniRef:UniRef90_UPI001F33AF27" ], "nt": "GTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGGATGGTTGGAAGTGGTGCGGCTGGAAGTGCTTCTAGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAAATCGCTCTTGAGATCAGCACCTGTCAGTGTTCCACCAAGCAGTACAAGTAATCAAACTTTAAGCTTATCTAACCCCGCTCCTGTGGGCCCACAAGCGGTTGTAAGCCAACCCGCGGGGGGTGCTACGGCAGCAGTGTCCGTCAATCGCACAGCGAGTGACACCGCCACCTTTAGCAAGTACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATTGTTCCGGGGTTGGAAAAATGA", "aa": "MYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSASSLQGNGSNSSGLKSLLRSAPVSVPPSSTSNQTLSLSNPAPVGPQAVVSQPAGGATAAVSVNRTASDTATFSKYLNTAQALHQMGVIVPGLEK", "aa_hexdigest": "8843f90dd8ff213ac750e6c3d8273e11", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "3-prime", "pseudogene": { "start": 435603, "stop": 436322, "observations": { "mutation": [ 436017 ], "stop": [ 436017 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F33AF27", "query_cov": 1.0, "subject_cov": 0.4726027397260274, "identity": 0.993, "score": 246.0, "evalue": 7.28e-80, "gene_start": 1, "gene_end": 138, "reference_start": 1, "reference_end": 138, "reference_sequence": "MYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSASSLQGNGSNSSGLKSLLRSAPVSVPPSSTSNQTLSLSNPAPVGPQAVVSQPAGGATAAVSVNRTASDTSTFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVASRRDATSTNLPHAAGASQTGLGTGSPREPALTATSQRAVTVVAGPLRAGNSSETDALPNVITQLYHTSTAQLAYLNGQIVVMGSDRVPSLWYWVVGEDQESGKATWWAKTELNWGTDKQKQFVENQLGFKDDSNSDSKNSNLK" }, "paralog": false, "identity": 0.9707112970711297, "score": 447.0, "evalue": 2.15e-161, "description": "Internal stop codon at 436017. Nonsense mutation around 436017.", "psc": { "uniref90_id": "UniRef90_UPI001F33AF27", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_P75414", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F33AF27", "UniRef:UniRef50_P75414" ] }, "pscc": { "uniref50_id": "UniRef50_P75414", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75414" ], "product": "Putative MgpC-like protein MPN_367" } }, "id": "GIHJHMOALN_792", "locus": "GCA009939745_00769" }, { "type": "cds", "sequence": "contig_1", "start": 436044, "stop": 436328, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTAGCCGACGGGATGCTACTTCCACTAACCTGCCCCATGCGGCAGGTGCTTCCCAAACGGGTTTGGGAACTGGTTCGCGGGGCGAACCAGCTTTAATCGCAACGTCACAGCGTGCCGTCACGGTGGTTGCTGGCCCCCTTCGTGCGGGCAATAGCAGTGAAACTGATGCCCTACCGAATGTCATCACCCAGCTCTATCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGAGTTCCGACCGGGTACCGAGTCTTTGGTATTGA", "aa": "MASRRDATSTNLPHAAGASQTGLGTGSRGEPALIATSQRAVTVVAGPLRAGNSSETDALPNVITQLYHTSTAQLAYLNGQIVVMSSDRVPSLWY", "aa_hexdigest": "2c98bc9a8d7a462fbbcd90005bfcb819", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9754.7728, "isoelectric_point": 8.291746330261233 }, "id": "GIHJHMOALN_793", "locus": "GCA009939745_00770" }, { "type": "cds", "sequence": "contig_1", "start": 436673, "stop": 436972, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC protein", "db_xrefs": [ "RefSeq:WP_236253458.1", "SO:0001217", "UniParc:UPI000AAE07FB", "UniRef:UniRef100_A0AB38W8G0", "UniRef:UniRef50_A0AB38W8G0", "UniRef:UniRef90_A0AB38W8G0" ], "nt": "GTGGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGTTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAG", "aa": "MENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSQNNEEVQTTYPVKSDQKATAKIASLINASPLNSYGDDGDKKAIYPEVRGRQLQNIFDDWKTNKSC", "aa_hexdigest": "b113843f3eac0f70a4ce1a1d7df36ae1", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000AAE07FB", "ncbi_nrp_id": "WP_236253458.1", "uniref100_id": "UniRef100_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniParc:UPI000AAE07FB", "RefSeq:WP_236253458.1", "UniRef:UniRef100_A0AB38W8G0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W8G0", "uniref90_id": "UniRef90_A0AB38W8G0" }, "psc": { "uniref90_id": "UniRef90_A0AB38W8G0", "product": "MgpC protein", "uniref50_id": "UniRef50_A0AB38W8G0" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "GIHJHMOALN_794", "locus": "GCA009939745_00771" }, { "type": "cds", "sequence": "contig_1", "start": 437534, "stop": 437908, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16526.10", "PFAM:PF01519.22", "PFAM:PF03670.18" ], "nt": "GTGAACACAGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAAATAAGATCGATAAGCTTGAAATTAAAGTTGATAAGCTAAAGAAGAAAGTCGATAAGCTGGAAGTTAAGGTTGATAAACTAGTCGAAACTGTTAATGCTCAAGGAGAAGATCTTAACAACTTCAAAGTTGAAGTGAGAGGAACACTTCAAAGCCAAGGCGAAACCCTGCAATTAATTTTGCAAACGCTACAAGACATGAGTAAGCGGCTTGATTCTGTTGAAGGACGTTTAGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTGGACAAAATCGATCCGCTAAAATAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLTKIENKIDKLEIKVDKLKKKVDKLEVKVDKLVETVNAQGEDLNNFKVEVRGTLQSQGETLQLILQTLQDMSKRLDSVEGRLDSMDGRLDSMETRLDKIDPLK", "aa_hexdigest": "63a3a07640eca0d545c9955a00cbaec6", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16526.10", "name": "CLZ", "length": 46, "aa_cov": 0.3709677419354839, "hmm_cov": 1.0, "evalue": 2.1839933843131974e-07, "score": 26.727916717529297, "start": 19, "stop": 64 }, { "id": "PF01519.22", "name": "DUF16", "length": 100, "aa_cov": 0.8064516129032258, "hmm_cov": 0.89, "evalue": 1.7966718975406421e-34, "score": 113.87850189208984, "start": 2, "stop": 101 }, { "id": "PF03670.18", "name": "UPF0184", "length": 45, "aa_cov": 0.3629032258064516, "hmm_cov": 1.0, "evalue": 2.3258906064566232e-06, "score": 23.44395637512207, "start": 12, "stop": 56 } ], "seq_stats": { "molecular_weight": 14444.300699999993, "isoelectric_point": 5.350731849670409 }, "id": "GIHJHMOALN_795", "locus": "GCA009939745_00772" }, { "type": "cds", "sequence": "contig_1", "start": 438040, "stop": 438132, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACAAATTTTTAAGTACCTTTGCTAATAATACAGAAGGCTATTCATCAGATATTAATCTCTTCATTTACTTGGAATATTTAATTAAGTAA", "aa": "MNKFLSTFANNTEGYSSDINLFIYLEYLIK", "aa_hexdigest": "b0e26d257c1f12e45212b995b9cda380", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3549.9948000000013, "isoelectric_point": 4.677076148986816 }, "id": "GIHJHMOALN_796", "locus": "GCA009939745_00773" }, { "type": "cds", "sequence": "contig_1", "start": 438196, "stop": 438465, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGATTGAATATTTCCAGATGACATACCAAAGTTTTAAGGATTTGTCAATTGAAGCTAAGTTGAGCTATACCTTTAATTGGTTTGGAGATTATTCTTCAGGTGATTTCACTGCTAAGAAAGGTGACAAGCATTATTTTGATCTTTTCCTTAAAATTAAATCTAATTCTGATCCAGGAAAACAATTTACTAAAGAAAAATTTCTTACTAAAGAAGAAAAAATTAAAGTTAATGGAAAAGAAATTACAAGAAATTTAGAGTGA", "aa": "MERKIEYFQMTYQSFKDLSIEAKLSYTFNWFGDYSSGDFTAKKGDKHYFDLFLKIKSNSDPGKQFTKEKFLTKEEKIKVNGKEITRNLE", "aa_hexdigest": "c8d1174f41623a12b9d71d61afaf0a69", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10627.933599999991, "isoelectric_point": 9.047638130187988 }, "id": "GIHJHMOALN_797", "locus": "GCA009939745_00774" }, { "type": "cds", "sequence": "contig_1", "start": 438553, "stop": 438897, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_369", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75412", "UniRef:UniRef90_P75412" ], "nt": "ATGTTCAACCCGAAATTCCAATTCAAGTTCTTATCCCAAAAAGGCTTTTTACAAAAAAAAAAAAAAAGCAAAATTAAAGAGTTATTTTTACTGTTGATGCGCAAAAAGAAGTTTTTGTCAAAGGTTTCCTTTGGTTCTTTATTTTTGTTATGTGGAACAGTTTTAAGCGCCTGTACTGGTATCCAAGCAGATCTTAGAAATCTTATTAAAGAAGCCACCGGTAAAGACATTGATGTGTATAAAGCAATTAAAACTACAGAAGGCAAAAAGAACATGATAACTTCTCTAAAGAAGTCTTACAAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCCTGA", "aa": "MFNPKFQFKFLSQKGFLQKKKKSKIKELFLLLMRKKKFLSKVSFGSLFLLCGTVLSACTGIQADLRNLIKEATGKDIDVYKAIKTTEGKKNMITSLKKSYKVNPKDTTKLLLDA", "aa_hexdigest": "77c0be0dd73a61cd6a9d2b3202fec012", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 438250, "stop": 438897, "observations": { "mutation": [ 438555 ], "stop": [ 438555 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75412", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 212.0, "evalue": 4.87e-67, "gene_start": 1, "gene_end": 114, "reference_start": 1, "reference_end": 114, "reference_sequence": "MFNPKFQFKFLSQKGFLQKKKKSKIKELFLLLMRKKKFLSKVSFGSLFLLCGTVLSACTGIQADLRNLIKEATGKDIDVYKAIKTTEGKKNMITSLKKSYKVNPKDTTKLLLDAWKQSAEKSELGIPDFDFDDVIYPASKDPFTMERKIEYFQMTYQSFKDLSIEAKLSYTFNWFGDYSSGDFTAKKGDKHYFDLFLKIKSNSDPGKQFTTEKFLTKEEKIKVNGKEITRNLEWIEFSASLSFSLKGKDDVSEKSVNKFLSTFANNTEGYSSDINLFIYLEYLIK" }, "paralog": false, "identity": 0.9906976744186047, "score": 425.0, "evalue": 2.2e-153, "description": "Internal stop codon at 438555. Nonsense mutation around 438555.", "psc": { "uniref90_id": "UniRef90_P75412", "product": "Uncharacterized lipoprotein MPN_369", "uniref50_id": "UniRef50_P75412", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75412", "UniRef:UniRef50_P75412", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75412" ], "product": "Uncharacterized lipoprotein MPN_369" } }, "id": "GIHJHMOALN_798", "locus": "GCA009939745_00775" }, { "type": "cds", "sequence": "contig_1", "start": 439279, "stop": 439398, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0AAN4XEA6" ], "nt": "ATGGTAGCTTACTTTGATCCAAAGGAAGACGGTACTTGTGAATTCATTATGAAAATTAAAACTGTAAATGAAGTTGCTGACACTGAGATAACAAATAAGGAAAAGCCTTTAGGCACTTGA", "aa": "MVAYFDPKEDGTCEFIMKIKTVNEVADTEITNKEKPLGT", "aa_hexdigest": "230972b44fd8b8153b632bd9c81fe607", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 439177, "stop": 439407, "observations": { "mutation": [ 439258, 439396 ], "stop": [ 439258, 439396 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAN4XEA6", "query_cov": 1.0, "subject_cov": 0.5131578947368421, "identity": 1.0, "score": 83.2, "evalue": 3.49e-20, "gene_start": 1, "gene_end": 39, "reference_start": 35, "reference_end": 73, "reference_sequence": "EKRKEHLNMKKVKALRNLFLEKASEKLEYPKIASMVAYFDPKEDGTCEFIMKIKTVNEVADTEITNKEKPLGTWKS" }, "paralog": false, "identity": 0.9605263157894737, "score": 145.0, "evalue": 3.27e-47, "description": "Internal stop codon at 439258, 439396. Nonsense mutation around 439258, 439396.", "psc": { "uniref90_id": "UniRef90_A0AAN4XEA6", "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAN4XEA6", "UniRef:UniRef50_A0A0H3DJX7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4407.0016000000005, "isoelectric_point": 4.554986000061036 }, "id": "GIHJHMOALN_799", "locus": "GCA009939745_00776" }, { "type": "cds", "sequence": "contig_1", "start": 439450, "stop": 439782, "strand": "+", "frame": 1, "gene": null, "product": "Adhesin P1-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQB0", "UniRef:UniRef90_A0AB33HQB0" ], "nt": "GTGTTAAAACTTGCTGTTGGTATTTTTATTTCCCCCACGCTCACCAGGTTTAGTACCGGGTTCAACCTCGCGGTGTCGGTGCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGCACAGGTATGGGAATAACCACCGGGGCGTGAATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTGGGTGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGA", "aa": "MLKLAVGIFISPTLTRFSTGFNLAVSVLDQVLDYVPWIGNGHRYGNNHRGVNDITAPKTGAGSSSGTSTNTSGSRSFLPTFSNVGVGLKANVQGTLGGSQTTTTGKDIPK", "aa_hexdigest": "106d63c92a7ffc5007284fc190e94f68", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 439450, "stop": 440085, "observations": { "mutation": [ 439780, 439813, 439828 ], "stop": [ 439780, 439813, 439828 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HQB0", "query_cov": 1.0, "subject_cov": 0.41509433962264153, "identity": 0.982, "score": 208.0, "evalue": 7.38e-66, "gene_start": 1, "gene_end": 110, "reference_start": 1, "reference_end": 110, "reference_sequence": "MLKLAVGIFISPTLTRFSTGFNLAGSVLDQVLDYVPWIGNGHRYGNNHRGVDDITAPKTGAGSSSGTSTNTSGSRSFLPTFSNVGVGLKANVQGTLGGSQTTTTGKDIPKWPTLDPAKLQLWTGAGWRNDKASNKQSDENHTTFKSATGSGQQGGSTTGGSAGNPDSLKQDKISKSGQNLTTQDGAPQSNSTTESASNYDHLPPPTSPPHPIDRTRCHSPTRTTRSAPSSSSAACWAASRCWWIEVGQMIPTNSKPPTKNGLTPT" }, "paralog": false, "identity": 0.9575471698113207, "score": 402.0, "evalue": 1.25e-144, "description": "Internal stop codon at 439780, 439813, 439828. Nonsense mutation around 439780, 439813, 439828.", "psc": { "uniref90_id": "UniRef90_A0AB33HQB0", "product": "Adhesin P1 homolog", "uniref50_id": "UniRef50_A0AB33HQB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HQB0", "UniRef:UniRef50_A0AB33HQB0" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HQB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQB0" ], "product": "Adhesin P1 homolog" } }, "id": "GIHJHMOALN_800", "locus": "GCA009939745_00777" }, { "type": "cds", "sequence": "contig_1", "start": 440166, "stop": 440447, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTGAATCGAAGTGGGTCAGATGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCTTACACCGACTTAAAGTCGGACCAAACCAAGCTCAACCTCCCCGCTTATGGTGAAGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNRSGSDDSNKFQATDQKWSYTDLKSDQTKLNLPAYGEVNGLLNPALVETYFGTTRAGGSGSNTTSSPGIGFKIPEQNNDSKAVLITPGLA", "aa_hexdigest": "2649d38c15df25edcb4701e922358f22", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9868.75109999999, "isoelectric_point": 5.125819969177246 }, "id": "GIHJHMOALN_801", "locus": "GCA009939745_00778" }, { "type": "cds", "sequence": "contig_1", "start": 440942, "stop": 441259, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_019830409.1", "SO:0001217", "UniParc:UPI000364214F", "UniRef:UniRef100_UPI000364214F", "UniRef:UniRef50_UPI000364214F", "UniRef:UniRef90_UPI000364214F", "PFAM:PF03257.18" ], "nt": "TTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAAGCCAAACCCCAATCCCTCAAAAAAACCACACCGGTGTTTGGGACGAATAGTGGTAACATTGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGACAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTGGCTTGTGGGGCAATTACCCAGTGGGGGTGGGGGGAATAGTAGTGAGGATATTAAAAGTGTGCAAGACACTCCTATTTATGATCTATATTCATTAGTATATTCTTTTTAA", "aa": "MDPNQVRTKLRQSFGTDHSTQAKPQSLKKTTPVFGTNSGNIGSVLSGGGAGGADSTNSVDLSPVERVSGWLVGQLPSGGGGNSSEDIKSVQDTPIYDLYSLVYSF", "aa_hexdigest": "f7bdc227d18dc70836735476be5a44cb", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000364214F", "ncbi_nrp_id": "WP_019830409.1", "uniref100_id": "UniRef100_UPI000364214F", "db_xrefs": [ "SO:0001217", "UniParc:UPI000364214F", "RefSeq:WP_019830409.1", "UniRef:UniRef100_UPI000364214F" ] }, "ips": { "uniref100_id": "UniRef100_UPI000364214F", "uniref90_id": "UniRef90_UPI000364214F" }, "psc": { "uniref90_id": "UniRef90_UPI000364214F", "uniref50_id": "UniRef50_UPI000364214F" }, "pscc": { "uniref50_id": "UniRef50_UPI000364214F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000364214F" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 71, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 1.8973057413593976e-26, "score": 88.32891082763672, "start": 1, "stop": 70 } ], "seq_stats": { "molecular_weight": 10906.823099999998, "isoelectric_point": 5.600084495544433 }, "id": "GIHJHMOALN_802", "locus": "GCA009939745_00779" }, { "type": "cds", "sequence": "contig_1", "start": 441786, "stop": 442277, "strand": "+", "frame": 3, "gene": null, "product": "Adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321", "UniRef:UniRef90_A0AB38W8Y4" ], "nt": "GTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALQVAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTP", "aa_hexdigest": "4bcf7107068d6cb7dd24f3a658688ab8", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "both", "pseudogene": { "start": 441636, "stop": 442433, "observations": { "mutation": [ 441753, 442275 ], "stop": [ 441753, 442275 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8Y4", "query_cov": 1.0, "subject_cov": 0.6150943396226415, "identity": 0.9940000000000001, "score": 254.0, "evalue": 2.71e-83, "gene_start": 1, "gene_end": 163, "reference_start": 51, "reference_end": 213, "reference_sequence": "MYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKNKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKNAEADTAKSNEKLQGAEATGSSTTSGSGQSTQRGGSSGDTKVKALQVAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTPWTPWLTTEQIHNDPAKFAASILILYDAPYARNRTVIDRVDHLDPKVMVDNYC" }, "paralog": false, "identity": 0.9886792452830189, "score": 475.0, "evalue": 1.64e-172, "description": "Internal stop codon at 441753, 442275. Nonsense mutation around 441753, 442275.", "psc": { "uniref90_id": "UniRef90_A0AB38W8Y4", "product": "Adhesin P1", "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8Y4", "UniRef:UniRef50_P75321" ] }, "pscc": { "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321" ], "product": "Uncharacterized protein MPN_462" } }, "id": "GIHJHMOALN_803", "locus": "GCA009939745_00780" }, { "type": "cds", "sequence": "contig_1", "start": 442496, "stop": 442939, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02917.20", "PFAM:PF22596.2" ], "nt": "ATGCCAAATCCTGTTAGATTTGTTTACCGTGTTGATTTGAGAAGCCCTGAAGAAATTTTTGAACATGGCTTTTCAACTTTAGGTGATGTGAGAAATTTCTTTGAACACATTCTCTCCACTAATTTTGGTAGAAGCTATTTTATTTCCACTTCAGAAACACCCACAGCAGCTATTCGCTTCTTTGGTAGCTGGTTACGGGAATATGTACCAGAGCACCCCAGAAGGGCTTACTTATATGAAATTCGTGCCGACCAACACTTTTACAATGCCCGCGCCACTGGGGAGAACTTGTTAGATTTAATGCGTCAAAGACAAGTAGTATTTGACTCTGGTGATCGAGAAATGGCACAAATGGGAATTAGAGCTTTACGCACTTCCTTTGCGTATCAACGTGAATGGTTTACCGATGGTCCAATTGCAGCAGCTAATGTCCGTAGTGCTTGA", "aa": "MPNPVRFVYRVDLRSPEEIFEHGFSTLGDVRNFFEHILSTNFGRSYFISTSETPTAAIRFFGSWLREYVPEHPRRAYLYEIRADQHFYNARATGENLLDLMRQRQVVFDSGDREMAQMGIRALRTSFAYQREWFTDGPIAAANVRSA", "aa_hexdigest": "991ddd04da404e2e6271f5eeddd807ba", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02917.20", "name": "Pertussis_S1", "length": 146, "aa_cov": 0.9727891156462585, "hmm_cov": 1.0, "evalue": 2.625474298680195e-83, "score": 274.9464111328125, "start": 3, "stop": 145 }, { "id": "PF22596.2", "name": "Scabin-like", "length": 114, "aa_cov": 0.7278911564625851, "hmm_cov": 0.9298245614035088, "evalue": 4.6042616378280084e-08, "score": 28.53148651123047, "start": 8, "stop": 114 } ], "seq_stats": { "molecular_weight": 17225.12139999999, "isoelectric_point": 6.919317436218263 }, "id": "GIHJHMOALN_804", "locus": "GCA009939745_00781" }, { "type": "cds", "sequence": "contig_1", "start": 443354, "stop": 443587, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22509.2" ], "nt": "ATGTTACAAGCAGATCCGCAAAATAACAATGTCTTTTTGGTCGAAGTAAACCCCAAACAAAAGTCCAGCTTTCCCCAAACCATCTTCTTTTGGGATGTTTATCAACGAATTTGTCTCAAGGATTTAACTGGTGCACAAATCAGTCTTTCGCTTACTGCCTTTACTACTCAGTATGCTGGTCAGCTCAAAGTGCACCTTAGTGTTAGCGCGGTTAATGCCGTGAACCAAAAGTGA", "aa": "MLQADPQNNNVFLVEVNPKQKSSFPQTIFFWDVYQRICLKDLTGAQISLSLTAFTTQYAGQLKVHLSVSAVNAVNQK", "aa_hexdigest": "6150e794e3b170ad6ba22a8f853ffe68", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22509.2", "name": "CARDS-D2_trefoil", "length": 84, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 6.033932892872781e-38, "score": 125.75577545166016, "start": 1, "stop": 77 } ], "seq_stats": { "molecular_weight": 8644.801099999999, "isoelectric_point": 8.931723976135256 }, "id": "GIHJHMOALN_805", "locus": "GCA009939745_00782" }, { "type": "cds", "sequence": "contig_1", "start": 443891, "stop": 444019, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22346.2" ], "nt": "ATGAAAACACCAGATGGACAGATATTCTATGATCTAAAAACTTCGAAAATCTTCTTTGTCCAGGACAACCAAAACGTGTTCTTTCTCCATAATAAACTCAACAAACAAACTGGTTACAGCTGGGATTGA", "aa": "MKTPDGQIFYDLKTSKIFFVQDNQNVFFLHNKLNKQTGYSWD", "aa_hexdigest": "04c19e688bad2cd46fd20e5ed0ad5150", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22346.2", "name": "CARDS_D3", "length": 42, "aa_cov": 1.0, "hmm_cov": 0.9761904761904762, "evalue": 1.0367479997672343e-17, "score": 59.96404266357422, "start": 1, "stop": 42 } ], "seq_stats": { "molecular_weight": 5057.648300000002, "isoelectric_point": 8.085834312438966 }, "id": "GIHJHMOALN_806", "locus": "GCA009939745_00783" }, { "type": "cds", "sequence": "contig_1", "start": 444041, "stop": 444271, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22346.2" ], "nt": "ATGAATGAGGACAAAGACGAAAACTTTAAATGGTACTTTTCGCGTGATGACCTTACCATTCCTTCCGTTGAAGGGCTTAACTTCCGCCACATTCGCTGTTACGCTGACAACCAGCAGTTAAAGGTGATCATAAGCGGTTCACGTTGGGGCGGTTGGTACTCCACTTACGATAAAGTTGAAAGTAATGTCGAAGATAAGATTTTGGTCAAAGATGGTTTTGATCGCTTTTAG", "aa": "MNEDKDENFKWYFSRDDLTIPSVEGLNFRHIRCYADNQQLKVIISGSRWGGWYSTYDKVESNVEDKILVKDGFDRF", "aa_hexdigest": "16378f58107b3c4b6ceee164ce8966fb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22346.2", "name": "CARDS_D3", "length": 68, "aa_cov": 0.8947368421052632, "hmm_cov": 1.0, "evalue": 4.80551438772093e-34, "score": 112.91462707519531, "start": 1, "stop": 68 } ], "seq_stats": { "molecular_weight": 9086.969899999996, "isoelectric_point": 4.984916114807128 }, "id": "GIHJHMOALN_807", "locus": "GCA009939745_00784" }, { "type": "cds", "sequence": "contig_1", "start": 444288, "stop": 445145, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BC084C", "UniRef:UniRef90_UPI0035BC084C" ], "nt": "TTGAAGTCCAACGAGAGTTCTAAACGTGGTGTTTTAAGTCAAGCTGCTAGTGGTGCGGTGGTTGGTTATGTAGAGAGACTAACTAACAAGAAGGTTTTCTTAAAGGACGAAATTAAGTTTGATGTTGAAAAGGATGGCATCAAGCTAGACTTAAAAGCCGACTTCTCGCTTGAACAAAATGGCTCTTTAGGTTTGAATGACCCAAATGATGTCAACAAATTGGCACAGGAAGCACAATCTTACACAGTTATGGTAAGTGATGGCGGTGCCCAAACTGACAATAATGCTGAAGGTGGTAATCTAAGAATTGCCTTAACTAAGAATGCATTCAACCCAAACCAAAGCACTACGGTTGATATTCCTTACAAAATAGAAAATAGAAGTGTGGGAAATAATAAGGAGCAGAAAACACTTGTGTTTGATTTCTCTGGACTCAATCCGTATGAGTACAACATGATTGTAGGGGCATTGTTTACCGACAGCTCATTTATTAATGATGCTTATGCACCAATTCAAAGTACCTTCCAAAGACAATTGAAGGAGTTCTTACAAGTTAAGTACGAAAACCAAGTTGGTGCTAATGGTTCATTTGACCTCTTCAAACCACGCTCATTGTCTAGCCAACAACTAGTTCAAGGTGAAAGATCACTAGATGGCTTTACCGTTGAATTGAATGCAAATGGTGGCAGTTTCAACTTCTTAACCCACGTAGATCCGTTGGTAGCTGGTTTAACTGTGGCTGCTATAGCTAGTGTAGTAGCTGCTGGTGCTGTAACTTATTTAGTAGTACGTCGTTACCGCAAGCGCAACGAGTTTGTCGACAAGATCTTTGCTTCTAACATTAGAGCAAAACAGTGA", "aa": "MKSNESSKRGVLSQAASGAVVGYVERLTNKKVFLKDEIKFDVEKDGIKLDLKADFSLEQNGSLGLNDPNDVNKLAQEAQSYTVMVSDGGAQTDNNAEGGNLRIALTKNAFNPNQSTTVDIPYKIENRSVGNNKEQKTLVFDFSGLNPYEYNMIVGALFTDSSFINDAYAPIQSTFQRQLKEFLQVKYENQVGANGSFDLFKPRSLSSQQLVQGERSLDGFTVELNANGGSFNFLTHVDPLVAGLTVAAIASVVAAGAVTYLVVRRYRKRNEFVDKIFASNIRAKQ", "aa_hexdigest": "eae73a543d62150cda61429de4350ec9", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0035BC084C", "query_cov": 1.0, "subject_cov": 0.9930313588850174, "identity": 0.996, "score": 540.0, "evalue": 1.2e-193, "valid": true, "uniref50_id": "UniRef50_UPI0035BC084C" }, "pscc": { "uniref50_id": "UniRef50_UPI0035BC084C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BC084C" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31304.719899999993, "isoelectric_point": 6.643023109436036 }, "id": "GIHJHMOALN_808", "locus": "GCA009939745_00785" }, { "type": "cds", "sequence": "contig_1", "start": 445224, "stop": 445517, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACGGAAATGAAAGCAATGGTCACTTCTATGTGGTAGTAGATGTAACTAACGCTAACAACCTTGGTAACCAACGTCGTATTGCTAACCCTAGAAACTATTTCTATTACCTAGAAGGTTTAGACAAGAACGCGCAAAGTACTTACATTATCCGTTTTGAAACAAAGGATAAGTTCTACTCACTCGATGCACTTAGGTTCGACAACAACGGTATATACGTCGATAATGCCTCAAGGGACGCTGTAATTCAAGCAAGACAAAAATCTAAACTGTATTTCAACACTAGTGATTGA", "aa": "MNGNESNGHFYVVVDVTNANNLGNQRRIANPRNYFYYLEGLDKNAQSTYIIRFETKDKFYSLDALRFDNNGIYVDNASRDAVIQARQKSKLYFNTSD", "aa_hexdigest": "81e8e0c4f9a1f5f5b261add98bfc2149", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11273.250799999985, "isoelectric_point": 7.946776008605957 }, "id": "GIHJHMOALN_809", "locus": "GCA009939745_00786" }, { "type": "cds", "sequence": "contig_1", "start": 445937, "stop": 446077, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCATTTACGCTAACGTTACCCTAGGACAAAAAGTAACTAAGGAAGTTAAAGTGTGGGATGGACAAAACTTCCAGTTCTTAGCAATTGAAAACAATGAAGACCAAGCTCAATGCTTTAGCAAACGAAAACAAAGCTGA", "aa": "MAIYANVTLGQKVTKEVKVWDGQNFQFLAIENNEDQAQCFSKRKQS", "aa_hexdigest": "4652c0566a95aeb7e765497ed42c4a6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5306.939300000001, "isoelectric_point": 7.884370613098144 }, "id": "GIHJHMOALN_810", "locus": "GCA009939745_00787" }, { "type": "cds", "sequence": "contig_1", "start": 446180, "stop": 446614, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_374", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75407", "UniRef:UniRef90_P75407" ], "nt": "GTGAAAGAGGTGATTGACACTAACACCACTGGAACTAACTTTAAGTTTGAAAACAGAAATTACAAACTACAAAACCAAAGCTTTAGTTTTGTCCGTTACAAAAACTTTAACTTAGATAAGCTGTTGATCCCTAAAACAAACGCTAATCTAAATTCAACTAGAAACGGTGTTTTAACTCAAGCACCTACCTTAGATTTTGTTTTAAATCCAGTTTTTGTTAATGCAATCACTAACGTTTACAACCTTTCTGAACAAGTAAAGGATTTAGAACAACGTTTAGAGAGCAAAAGTAACGAAAGTGAAAAAGCTGGCATTAACAGCGTTTTAACGCAAATTAAAAACAAGCAAGTTGATTACCTAAAGGTAAAAGAATACATTAGTAGCCTAGATAAAGATACCAATAAAGTAAGTACTCCAATTAACTCAACCGGTTGA", "aa": "MKEVIDTNTTGTNFKFENRNYKLQNQSFSFVRYKNFNLDKLLIPKTNANLNSTRNGVLTQAPTLDFVLNPVFVNAITNVYNLSEQVKDLEQRLESKSNESEKAGINSVLTQIKNKQVDYLKVKEYISSLDKDTNKVSTPINSTG", "aa_hexdigest": "8cec4020a07a8bbd04c976782f08d0e6", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 445925, "stop": 446614, "observations": { "mutation": [ 445939, 446116, 446182 ], "stop": [ 445939, 446116, 446182 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75407", "query_cov": 1.0, "subject_cov": 0.62882096069869, "identity": 1.0, "score": 272.0, "evalue": 3.86e-91, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MKEVIDTNTTGTNFKFENRNYKLQNQSFSFVRYKNFNLDKLLIPKTNANLNSTRNGVLTQAPTLDFVLNPVFVNAITNVYNLSEQVKDLEQRLESKSNESEKAGINSVLTQIKNKQVDYLKVKEYISSLDKDTNKVSTPINSTGWVKWYQEANKELGLNLNQEPKDWDQFLKLVASYFSMAIYANVTLGQKVTKEVKVWDGQNFQFLAIENNEDQAQCFSKRKQSWNYC" }, "paralog": false, "identity": 0.982532751091703, "score": 446.0, "evalue": 7.96e-162, "description": "Internal stop codon at 445939, 446116, 446182. Nonsense mutation around 445939, 446116, 446182.", "psc": { "uniref90_id": "UniRef90_P75407", "product": "Uncharacterized protein MPN_374", "uniref50_id": "UniRef50_P75407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75407", "UniRef:UniRef50_P75407" ] }, "pscc": { "uniref50_id": "UniRef50_P75407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75407" ], "product": "Uncharacterized protein MPN_374" } }, "id": "GIHJHMOALN_811", "locus": "GCA009939745_00788" }, { "type": "cds", "sequence": "contig_1", "start": 446960, "stop": 447151, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_375", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75406", "UniRef:UniRef90_P75406" ], "nt": "GTGCTCATTGAAAGGGGTGAACTTCACCGTCAAGCAGATTTAAAAAAATCTGACCCCGCAACAAACGAAACTTCCAAAACCGTTTGGGGTAGCATTAAAGAAAAGCTGTTACAAAACGTCAACAACCTCCACTCAGAGAAAGGAGTATTCTTATGGTTCCGTCAATCTGGTTTTACAACTACTAGAAACTAG", "aa": "MLIERGELHRQADLKKSDPATNETSKTVWGSIKEKLLQNVNNLHSEKGVFLWFRQSGFTTTRN", "aa_hexdigest": "fed5dd3bc384590074c0c74989a3d5c8", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 446960, "stop": 447349, "observations": { "mutation": [ 447193 ], "stop": [ 447193 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75406", "query_cov": 1.0, "subject_cov": 0.4883720930232558, "identity": 0.9840000000000001, "score": 129.0, "evalue": 2.32e-37, "gene_start": 1, "gene_end": 63, "reference_start": 67, "reference_end": 129, "reference_sequence": "MSFGLVGTVNNNGWKSPFRHETKYRAGYDKFKYYKTHYRGAKKAGTNDDRWRWTAWFDLDFAHQKIVLIERGELHRQADLKKSDPATNETSKTVWGSIKEKLLQNVNNLHSEKGVFLWFRQSGFTTTRN" }, "paralog": false, "identity": 0.9922480620155039, "score": 273.0, "evalue": 2.39e-96, "description": "Internal stop codon at 447193. Nonsense mutation around 447193.", "psc": { "uniref90_id": "UniRef90_P75406", "product": "Uncharacterized protein MPN_375", "uniref50_id": "UniRef50_P75406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75406", "UniRef:UniRef50_P75406" ] }, "pscc": { "uniref50_id": "UniRef50_P75406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75406" ], "product": "Uncharacterized protein MPN_375" } }, "id": "GIHJHMOALN_812", "locus": "GCA009939745_00789" }, { "type": "cds", "sequence": "contig_1", "start": 447151, "stop": 447291, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAAAATACCGAGCAGGGTATGATAAGTTCAAGTATTACAAAACCCACTACCGGGGTGCAAAAAAAGCTGGAACCAATGATGATCGATGGAGATGAACTGCTTGGTTTGACCTTGACTTTGCCCACCAAAAGATAG", "aa": "MKQNTEQGMISSSITKPTTGVQKKLEPMMIDGDELLGLTLTLPTKR", "aa_hexdigest": "87d9816f44c48cfed52cef25a76fc9b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5061.912899999999, "isoelectric_point": 8.1404390335083 }, "id": "GIHJHMOALN_813", "locus": "GCA009939745_00790" }, { "type": "cds", "sequence": "contig_1", "start": 447867, "stop": 449459, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_376", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75405", "UniRef:UniRef90_P75405" ], "nt": "ATGAAGGTTTGGGATGGTGCTAAGTTTGAGTTCATCCCGATTGAAAACACTGAAAAACAATCAGAACAACTAGCAAATGAAAACCGCGCTGAGATTGCTGTAAGCGCCATTGGTTTTCAAGATGAAGGCGGTTTACGTGATGCTAGTTTTATCAATAAGGTAGCTCTAACCCCTAAGAGTAGTAAAACTAAAATTGCTAACGGTGATGCTAGCAAAATAGAAAAAGCAGCCAACGAAATTTCTTATAAGTACCATTACCGACAAAACTTTAAACAAGCTAGCTGGGACAAACAAAACTCACAAACCAAGAGTATTGTAGTACAAAGCACTGATTTGAACGATGAGCGCGAACGCTTCCAAAAGGACATTAACAACTATTTAAAGGTACAAGGCATCTCTGAAACTGAAATTAAAGTAAATGCTGTTCATAAAGTTGATGCGATGTTAAACGCCCGCAAAAGTGATGATCCGAAGCTCGCTAGCGTTCAAAGTACGGCAAACAAGTATGGTTTAAACCTACGATCTAACCCATACACTGGTCAGTTTTATGTAGTAGTGGATGTAACTAATGCCAATGACTTGGGTAACCAACGCCGGGCAAATAACGCTAAGAGTTACTTTTACTATATTGAAGGGTTGGACAAAGGAGCACAAAGTTCGTACTTAGTGCGCTTTGAAAACAAACAAAAGCTGTATTCACTCGAATCACTAGCGGTTGATAGTCGTGGCCTTTATGTGAAAAATGTGTCCAAGGATGCAATTATCCAAGCCAAGCAAAACCAAAACCTTTATCTCGATACTCATAACTGGAACGCTGCTTTGAAGGCTAACCTCACCAACGCTGAACTTACTTTGCCTACAGCAAGTGCTGACAATTCAGCTAAACTATCTACACCTAACGCTGAGAATGATGAGGGTTTTTTGAGTGAAAATGTCAGTGGTTCGATCTTAGGCTATGTTGAGCGAATGACTGGTAAAAAGCTCTTTTTAAAGGAAAGGGTGAGCTTTAATAAGGAAGACAAAAATAATTTAAAGCTACGTTTAACATCGAACTTTACCTTAGACAAGAAGGGTAATTTAGAGGTTAAAGACCCAAGTGTAATTAACCAAATTGTAGAAGAAGCTAAGGGTTACAATGTCTTGGTTTCAGAAGAAAAAGGTGATGATCCAGAATCAGACAAAAACATCTTCAAGATTACTTTAACCACCAACCCTGAACAAAGTACCGTTATTAAACTGCCTTACTGGATTGTTACCAAGAAATCCAAAACTAACAAGGATGGTACGGTTAGAGAGCAGAAAAACTTGGTGTTTGACTTCTCTAACCTAAACAACTTCGAATACAACACAGTTGTTAGTCTGTTGTTTACCGACAGCTCCTTTATTAAGAATGCCTACGCTCCATTGCAAACAGAATTTAGAAAGCAGCTCAAAACCGTTTTAGAACACAAATACCAAGCACCAATTAAGACTGGTCAACTACCTTTATTGACCAAGGTACAATTGGCAAATAACCAAAAACAAATTGATAACTTCACCTTTGATCTACACAAAAACATTTTCAATAAAGAAGACATCAATAAGATTAACTGA", "aa": "MKVWDGAKFEFIPIENTEKQSEQLANENRAEIAVSAIGFQDEGGLRDASFINKVALTPKSSKTKIANGDASKIEKAANEISYKYHYRQNFKQASWDKQNSQTKSIVVQSTDLNDERERFQKDINNYLKVQGISETEIKVNAVHKVDAMLNARKSDDPKLASVQSTANKYGLNLRSNPYTGQFYVVVDVTNANDLGNQRRANNAKSYFYYIEGLDKGAQSSYLVRFENKQKLYSLESLAVDSRGLYVKNVSKDAIIQAKQNQNLYLDTHNWNAALKANLTNAELTLPTASADNSAKLSTPNAENDEGFLSENVSGSILGYVERMTGKKLFLKERVSFNKEDKNNLKLRLTSNFTLDKKGNLEVKDPSVINQIVEEAKGYNVLVSEEKGDDPESDKNIFKITLTTNPEQSTVIKLPYWIVTKKSKTNKDGTVREQKNLVFDFSNLNNFEYNTVVSLLFTDSSFIKNAYAPLQTEFRKQLKTVLEHKYQAPIKTGQLPLLTKVQLANNQKQIDNFTFDLHKNIFNKEDINKIN", "aa_hexdigest": "870cdcc48cbd6877478a28c061d72697", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 447729, "stop": 449759, "observations": { "mutation": [ 447764, 447788, 447869, 449546, 449654 ], "stop": [ 447764, 447788, 447869, 449546, 449654 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75405", "query_cov": 1.0, "subject_cov": 0.4649122807017544, "identity": 1.0, "score": 1013.0, "evalue": 0.0, "gene_start": 1, "gene_end": 530, "reference_start": 565, "reference_end": 1094, "reference_sequence": "MKRLQYRFLLFGFALGSFGWFVASSAFTSVVKSNNETLSSDGGVHMRYLKGKGDVLSILKSKSPAITQRFDLKWNNGKKTEVYLNNVNNYISESLYAQEALKQIERTAQLSIQNGELPDVKVYTNSGASFGLVGWHTDNGRTWAFRHENKYDKNVHFSWNQTYYKYSNRERTASNPYYWKWVAWFDLGYANQRIGLVENDEYHIDKRVPEPTPRKWDKNKPLWGDIRSKILYSAERLDPDKGIFIWFNQTGFNTKGTKGWANSGFFTDFWDTNNNPNAFTTNITSEGGNSNWHSPDWGSHTTDTRFFLKLEPYSKLFYKENGQERSITVSDYIRKAKSTKTNYQWVNKNQIKTLVRKTRSIDLGLGSAVRQTYTTKSDIASNQQLKYKLKDSTFSIDTYNNFKLDKLLVPKTNEDATAIKNGVFVKQPTLSFDFNPVLTNAIVNIHNLFAQTLDLKEHLKSDQPYNESDKAAINKVIEQIKNKEVDYIQVADFIGKLKNWSQNPGSIESKGENTAQWYADAKREFGLNLNDDVNTWTQLSSLIASYFSKDIFANVKLNGAKERRMKVWDGAKFEFIPIENTEKQSEQLANENRAEIAVSAIGFQDEGGLRDASFINKVALTPKSSKTKIANGDASKIEKAANEISYKYHYRQNFKQASWDKQNSQTKSIVVQSTDLNDERERFQKDINNYLKVQGISETEIKVNAVHKVDAMLNARKSDDPKLASVQSTANKYGLNLRSNPYTGQFYVVVDVTNANDLGNQRRANNAKSYFYYIEGLDKGAQSSYLVRFENKQKLYSLESLAVDSRGLYVKNVSKDAIIQAKQNQNLYLDTHNWNAALKANLTNAELTLPTASADNSAKLSTPNAENDEGFLSENVSGSILGYVERMTGKKLFLKERVSFNKEDKNNLKLRLTSNFTLDKKGNLEVKDPSVINQIVEEAKGYNVLVSEEKGDDPESDKNIFKITLTTNPEQSTVIKLPYWIVTKKSKTNKDGTVREQKNLVFDFSNLNNFEYNTVVSLLFTDSSFIKNAYAPLQTEFRKQLKTVLEHKYQAPIKTGQLPLLTKVQLANNQKQIDNFTFDLHKNIFNKEDINKINWPLIAITFTGSAALLSTIIASGVVLHRWRKSRKHFWEQMLKARKVK" }, "paralog": false, "identity": 0.9911242603550295, "score": 1306.0, "evalue": 0.0, "description": "Internal stop codon at 447764, 447788, 447869, 449546, 449654. Nonsense mutation around 447764, 447788, 447869, 449546, 449654.", "psc": { "uniref90_id": "UniRef90_P75405", "product": "Uncharacterized protein MPN_376", "uniref50_id": "UniRef50_P75405", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75405", "UniRef:UniRef50_P75405", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75405" ], "product": "Uncharacterized protein MPN_376" } }, "id": "GIHJHMOALN_814", "locus": "GCA009939745_00791" }, { "type": "cds", "sequence": "contig_1", "start": 449652, "stop": 449927, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAAAAACCAACGAGGATGCTACGGCAATTAAGAATGGTGTTTTTGTCAAACAACCAACACTGTCCTTTGACTTTAACCCCGTTTTGACAAACGCCATTGTCAACATTCACAATCTGTTTGCGCAAACACTTGATTTAAAGGAGCATTTAAAGTCTGATCAACCTTACAATGAAAGTGACAAAGCAGCAATCAATAAGGTTATAGAACAAATCAAAAATAAGGAAGTTGACTACATTCAAGTAGCTGATTTTATTGGCAAGCTGAAAAATTGA", "aa": "MPKTNEDATAIKNGVFVKQPTLSFDFNPVLTNAIVNIHNLFAQTLDLKEHLKSDQPYNESDKAAINKVIEQIKNKEVDYIQVADFIGKLKN", "aa_hexdigest": "c56e106ad43a8b3a1c109fc22c6d28d9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10286.595499999992, "isoelectric_point": 6.056785774230956 }, "id": "GIHJHMOALN_815", "locus": "GCA009939745_00792" }, { "type": "cds", "sequence": "contig_1", "start": 450261, "stop": 450608, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTGGTTTGATTTGGGTTATGCCAACCAACGCATAGGTTTGGTGGAAAATGATGAATATCATATTGACAAGCGTGTTCCAGAACCAACTCCCAGAAAATGGGATAAAAACAAACCTTTATGGGGTGATATTCGCTCGAAGATTCTCTACAGTGCTGAGCGGTTAGATCCAGATAAGGGAATCTTCATCTGGTTCAACCAAACTGGTTTTAACACCAAGGGTACTAAGGGCTGGGCTAACAGTGGCTTTTTTACCGATTTTTGGGATACCAACAATAACCCCAATGCCTTTACCACCAACATCACTTCTGAAGGTGGTAACTCCAATTGGCACAGTCCTGATTGA", "aa": "MAWFDLGYANQRIGLVENDEYHIDKRVPEPTPRKWDKNKPLWGDIRSKILYSAERLDPDKGIFIWFNQTGFNTKGTKGWANSGFFTDFWDTNNNPNAFTTNITSEGGNSNWHSPD", "aa_hexdigest": "1557b8f18486d770c223a0cfc85380d7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13330.473299999983, "isoelectric_point": 5.623558998107909 }, "id": "GIHJHMOALN_816", "locus": "GCA009939745_00793" }, { "type": "tRNA", "sequence": "contig_1", "start": 451416, "stop": 451492, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 74.9, "nt": "GCGCCCATAGCTCAATTGGATAGAGTGTCTGGCTTCGGACCAGAAGGTTATGGGTTCAAGTCCTATTGGGCGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 451456, 451458 ], "id": "GIHJHMOALN_31", "locus": "GCA009939745_00794" }, { "type": "cds", "sequence": "contig_1", "start": 451578, "stop": 451802, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_377", "db_xrefs": [ "RefSeq:WP_014325500.1", "SO:0001217", "UniParc:UPI0001DD63C9", "UniRef:UniRef100_A0AAX0SQJ9", "UniRef:UniRef50_Q9EXD3", "UniRef:UniRef90_Q9EXD3" ], "nt": "ATGTCAAAGGATAAAAAAAATAAGGTTGAACAGCTTGAACCAGTAGACCTTTTTGAGCGGACTAAGCTAGAGGACACCCAAGTTTTAAATGATGTTGAACTAGATGACATCAAGAAACTAGAAGAGTTGAAAAAAGAATTGGAAAACACCTTTGATCCACGTACCAGAATTGAAATTAAACGTGAAATCAAAGAGTTGGAACGTAAACTAAGAAGGAACCGTTAG", "aa": "MSKDKKNKVEQLEPVDLFERTKLEDTQVLNDVELDDIKKLEELKKELENTFDPRTRIEIKREIKELERKLRRNR", "aa_hexdigest": "b2fb131c574bc736a14e56dd6c917ad9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63C9", "ncbi_nrp_id": "WP_014325500.1", "uniref100_id": "UniRef100_A0AAX0SQJ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63C9", "RefSeq:WP_014325500.1", "UniRef:UniRef100_A0AAX0SQJ9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQJ9", "uniref90_id": "UniRef90_Q9EXD3" }, "psc": { "uniref90_id": "UniRef90_Q9EXD3", "product": "Uncharacterized protein MPN_377", "uniref50_id": "UniRef50_Q9EXD3" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD3" ], "product": "Uncharacterized protein MPN_377" }, "genes": [], "id": "GIHJHMOALN_817", "locus": "GCA009939745_00795" }, { "type": "cds", "sequence": "contig_1", "start": 451806, "stop": 452342, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTAACCTCCACACTAACTCTTACTATAACTTCCTCAACTCCACACTTTCACCGCAAAAGCTAGTTGACCTAGCTGTTCAAGACCAACAGGTAGCTGTTTGTTTAACCGATCCCAACTTATTTGGTGCTACCGAGTTTTTTTTGGCCTGTCAAAAAGCGCACATTAAACCCTTAATTGGGTTGAGTGTTACTGTTCGACATTACGAACAAAACGTTAATTTACTAGTAATTGCCCAAACCAACCGGGGTTATCAGAACCTAATGTGCTTAGCGTTAGTTAAAGACCAACCAGATTTGCAATTAGAACCCTTTTTGGATGGTAATGTGGTAATTATTTGCACACAAACTGAACTGCGCCTCAACACCGCAAACCCCGTTTACTTAGCACATGGTTTGAGTGGTCGTTATCCTAAAATTGCAGTAACACAAAAACCAGTTAAGTGTCAAAACACCAATAAGGACTTAACACTGCTCTTAACACTTAAGCAAATTAGTCAAATTAACAGCGAACACTTCCAGCCACTGGAATGA", "aa": "MFVNLHTNSYYNFLNSTLSPQKLVDLAVQDQQVAVCLTDPNLFGATEFFLACQKAHIKPLIGLSVTVRHYEQNVNLLVIAQTNRGYQNLMCLALVKDQPDLQLEPFLDGNVVIICTQTELRLNTANPVYLAHGLSGRYPKIAVTQKPVKCQNTNKDLTLLLTLKQISQINSEHFQPLE", "aa_hexdigest": "3209869950fa494f0036ef272393cc74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20022.923600000006, "isoelectric_point": 6.943928718566894 }, "id": "GIHJHMOALN_818", "locus": "GCA009939745_00796" }, { "type": "cds", "sequence": "contig_1", "start": 452529, "stop": 453929, "strand": "+", "frame": 3, "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "COG:COG0587", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0008408", "SO:0001217", "UniRef:UniRef50_Q49405", "UniRef:UniRef90_P75404" ], "nt": "GTGTCACACAACGATGCACAAATGTTAAGCGAGCAGTGTCAACTAGCCTTAACTGAATTTTTAAAGCTCAATCCGCAATTAAACAAACAGCTCTATGAAGAGCGGTTAGCCAAAGAATTGGAGATAATTAACTCACTCCACTTTGCTAGTTACTTTTTAGTGGTGAGCGACTTAGTTCAGTTTGCCCATAATAACGATATCCTCATCGGTCCTGGTCGAGGATCTGCAGTGGGTTCTTTAGTGGCCTTTCTGCTCAAAATTACCCAAATTGATCCAGTAGCCAACAATCTCATTTTTGAGCGCTTTATTTCCCGTCACCGCCAGGGCTTACCTGACATTGACATTGACATTATGGAAACTAAACGTGATTTAGTAATTGACTATGTCATGCAAAAATATGGCCGGGAACAGTGTGCACAAATAGTTACTTTCCAAAAATTTAAGACCCGCTCTGCGCTGCGTGATGTCGGTAAGGTATTTAAACACATTGAGGGTGCTGAAGATTTACTGGGTAAACTACCAAAAGACAAAAGCTTATTGGAGCTTGATGTAAACGGTGTTACTGATCCGGTTTTACAACTTAGTTTAAAGAGCTTTCGCTTGTTGTGGGAGGTGGCGCGTGAAATTATTAACTTTCCGCGCCAACCAAGCATTCACGCTTCTGGTGTTGTGATTGTCACTGAACCTTTAATTACCACGATCCCTTTAATGGTGGGTAATAACAACAACTACGTCACCCAAGTGTCGATGGACTGGTTAGAATGGTACAACCTCAATAAGTTTGACTTATTGGGTTTAATTAACCTCACCATGATCCATGAGGTTGTGCAAGCGGTAAAACCCAAGGAAGTTAGCGTACAGCAATTCCTCCAACAAATACCGCTAGATGATGAAGCTACTTTTACTAACCTCACTAACCAAGCCACTTTAGGTGTTTTTCAACTAGAGTCGTTTGGGATGAAAAAGGTGCTCAAACAAATTAAACCGCACAACCTCCATGATTTAGCAATTGTTTTAGCACTTTACCGCCCCGGTCCACAGGACAACATTAATACCTTTATTGCTAACCGTAACTTAGGGTTTGACACATCAGACATCGATCCGCGAATTTTGCCAATCCTCAAAGAAACTTACGGAGTGTTAATCTTTCAAGAACAGGTTATTAACATCGCCAAAACTGTTGCTAACTATTCATTGGAAACCGCTGATAGCTTTCGCCGTGCCATTTCCAAAAAAAACTTGCAAGTAATTCAGGACAACATGCGCAGCTTTTATGAAGGCGCGCTAGCCAATAACTTTAGCTTAAAAGCAGCGACTACTATCTTTAATTACATCCAGCGCTTTGCTGGATATGGTTTTAACTTGTCCCACGCCTTGGGCTATGCTTTATTGAGTTACTGA", "aa": "MSHNDAQMLSEQCQLALTEFLKLNPQLNKQLYEERLAKELEIINSLHFASYFLVVSDLVQFAHNNDILIGPGRGSAVGSLVAFLLKITQIDPVANNLIFERFISRHRQGLPDIDIDIMETKRDLVIDYVMQKYGREQCAQIVTFQKFKTRSALRDVGKVFKHIEGAEDLLGKLPKDKSLLELDVNGVTDPVLQLSLKSFRLLWEVAREIINFPRQPSIHASGVVIVTEPLITTIPLMVGNNNNYVTQVSMDWLEWYNLNKFDLLGLINLTMIHEVVQAVKPKEVSVQQFLQQIPLDDEATFTNLTNQATLGVFQLESFGMKKVLKQIKPHNLHDLAIVLALYRPGPQDNINTFIANRNLGFDTSDIDPRILPILKETYGVLIFQEQVINIAKTVANYSLETADSFRRAISKKNLQVIQDNMRSFYEGALANNFSLKAATTIFNYIQRFAGYGFNLSHALGYALLSY", "aa_hexdigest": "09e8ed2c51ef07f67ac42e50f862bde9", "start_type": "GTG", "rbs_motif": null, "genes": [ "dnaE" ], "truncated": "both", "pseudogene": { "start": 452229, "stop": 454232, "observations": { "mutation": [ 452340, 452493, 453927, 453936, 453975 ], "stop": [ 452340, 452493, 453927, 453936, 453975 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75404", "query_cov": 1.0, "subject_cov": 0.5344036697247706, "identity": 0.996, "score": 907.0, "evalue": 0.0, "gene_start": 1, "gene_end": 466, "reference_start": 242, "reference_end": 707, "reference_sequence": "MFVNLHTNSYYNFLNSTLSPQKLVDLAVQDQQVAVCLTDPNLFGATEFFLACQKAHIKPLIGLSVTVRHYEQNVNLLVIAQTNRGYQNLMCLALVKDQPDLQLEPFLDGNVVIICTQTELRLNTANPVYLAHGLSGRYPKIAVTQKPVKCQNTNKDLTLLLTLKQISQINSEHFQPLEWKLSRSLNEIQLDPPLLQQLRHQPFLSQKAAQQIFSEEELGNLQKLVEQSQWDLTKLKASSLQVSHNDAQMLSEQCQLALTEFLKLNPQLNKQLYEERLAKELEIINSLHFASYFLVVSDLVQFAHNNDILIGPGRGSAVGSLVAFLLKITQIDPVANNLIFERFISRHRQGLPDIDIDIMETKRDLVIDYVMQKYGREQCAQIVTFQKFKTRSALRDVGKVFNHIEGAEDLLGKLPKDKSLLELDVNGVTDPVLQLSLKSFRLLWEVAREIINFPRQPSIHASGVVIVTEPLITTIPLMVGNNNNYVTQVSMDWLEWYNLNKFDLLGLINLTMIHEVVQAVKPKEVSVQQFLQQIPLDDEATFTNLTNQATLGVFQLESFGMKKVLKQIKPHNLHDLAIVLALYRPGPQDNINTFIANRNLGFDTSDIDPRILPILKETYGVLIFQEQVINIAKTVANYSLETADSFRRAISKKNLQVIQDNMRSFYEGALANNFSLKAATTIFNYIQRFAGYGFNLSHALGYALLSYWTAWLKTHYFEQFNLWWLNHEQGKKEKQKQLLNEFISSGYEICPPLINKAKSDFSVQDKKLYLGFKLINGIGDKQAHALEHVQEVLKQNPNLSLIATVNLCLSKTVGGLELKDITLLQQAGCFNCFNYTVDFNLAKSFWVQSNHELFPKIPLDQPPVINWKSFGF" }, "paralog": false, "identity": 0.9910044977511244, "score": 1290.0, "evalue": 0.0, "description": "Internal stop codon at 452340, 452493, 453927, 453936, 453975. Nonsense mutation around 452340, 452493, 453927, 453936, 453975.", "psc": { "uniref90_id": "UniRef90_P75404", "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q49405", "cog_id": "COG0587", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75404", "EC:2.7.7.7", "UniRef:UniRef50_Q49405", "COG:COG0587", "COG:L", "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q49405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49405" ], "product": "DNA polymerase III subunit alpha" } }, "id": "GIHJHMOALN_819", "locus": "GCA009939745_00797" }, { "type": "cds", "sequence": "contig_1", "start": 454161, "stop": 454406, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACATGTTCAGGAAGTTTTAAAGCAAAACCCCAATTTATCCTTAATAGCTACCGTTAATTTGTGTTTGAGCAAAACGGTAGGGGGTTTAGAACTTAAAGACATTACATTGCTCCAACAAGCAGGTTGTTTTAATTGCTTTAATTACACGGTTGACTTTAATTTGGCCAAAAGCTTTTGGGTGCAAAGTAACCATGAATTATTTCCAAAAATACCACTAGATCAACCACCCGTAATTAACTGA", "aa": "MEHVQEVLKQNPNLSLIATVNLCLSKTVGGLELKDITLLQQAGCFNCFNYTVDFNLAKSFWVQSNHELFPKIPLDQPPVIN", "aa_hexdigest": "014008d0d84c6781f1abcd1553ee6881", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9129.473399999993, "isoelectric_point": 5.453553581237792 }, "id": "GIHJHMOALN_820", "locus": "GCA009939745_00798" }, { "type": "cds", "sequence": "contig_1", "start": 454740, "stop": 455303, "strand": "+", "frame": 3, "gene": "polA", "product": "5'-3' exonuclease", "db_xrefs": [ "COG:COG0258", "COG:L", "EC:3.1.11.-", "GO:0003677", "GO:0008409", "GO:0017108", "GO:0033567", "SO:0001217", "UniRef:UniRef50_P75403", "UniRef:UniRef90_P75403" ], "nt": "GTGTGTGAACAAGACGGGTTTGAGGCAGATGATCTAATTGGTAGTTATGCGAGGTTAATGACACAAAACAATGTGGCAGTAGACATCTACAGCTCTGACCGTGATTTACTACAGTTAGTCGATAGTATGACTAGTGTTTGGCTTTGTGTAAAGGGCACAAAAGAGATGAAGGAATATAACACTGACAACTTTGCTGAGCAGTTTTTTGGCTTAACACCAAAACAAGTGATTGAATATAAAGGTTTGGTAGGGGATAACAGTGATAACCTCACTGGCATTAAGGGAATTGGTCCGAAAAAAGGGATTGATCTACTCAAACAATACGGTACCATTGACAATATTTTTGCTAACTTTGACAAGCTATCCAAAGCATTGCAAACGATTCTCCAAGGCCAAATTGACACTGCTAAGAAATTCAGCTTTTTAGCGAGCATTAAAACAGATATTAAACTTAATGACGATATTGTCCACGCTGCTTTAAAACCAATCGATAAACAAGCTTTATTAGAGCTTTTAGATAAATATGGCATTAAAGCACTAGCGCAAAAATTTAGTCAACTGTAA", "aa": "MCEQDGFEADDLIGSYARLMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEMKEYNTDNFAEQFFGLTPKQVIEYKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTAKKFSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL", "aa_hexdigest": "daef5f8d5d43e87d2333fe85ad5f3a91", "start_type": "GTG", "rbs_motif": null, "genes": [ "polA" ], "truncated": "5-prime", "pseudogene": { "start": 454440, "stop": 455303, "observations": { "mutation": [ 454488, 454563 ], "stop": [ 454488, 454563 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75403", "query_cov": 1.0, "subject_cov": 0.6426116838487973, "identity": 0.995, "score": 369.0, "evalue": 8.07e-128, "gene_start": 1, "gene_end": 187, "reference_start": 105, "reference_end": 291, "reference_sequence": "MKNAILIDGNSLAYRAYFATWQQVEFAKLHNLPFNNAIRTMLMMCWNLLQSKQYDYGVISFDTKAPTFRDQLYSEYKSKRSKTPSELLVQIPVVKESLRHLGFLVCEQDGFEADDLIGSYARLMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEMKEYNTDNFAEQFFGLTPKQVIEYKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTAKKFSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL" }, "paralog": false, "identity": 0.9930313588850174, "score": 560.0, "evalue": 2.43e-205, "description": "Internal stop codon at 454488, 454563. Nonsense mutation around 454488, 454563.", "psc": { "uniref90_id": "UniRef90_P75403", "gene": "polA", "product": "5'-3' exonuclease", "ec_ids": [ "3.1.11.-" ], "uniref50_id": "UniRef50_P75403", "cog_id": "COG0258", "cog_category": "L", "go_ids": [ "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75403", "EC:3.1.11.-", "UniRef:UniRef50_P75403", "COG:COG0258", "COG:L", "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ] }, "pscc": { "uniref50_id": "UniRef50_P75403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75403" ], "product": "5'-3' exonuclease" } }, "id": "GIHJHMOALN_821", "locus": "GCA009939745_00799" }, { "type": "cds", "sequence": "contig_1", "start": 455303, "stop": 456136, "strand": "+", "frame": 2, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "COG:COG0266", "COG:L", "EC:3.2.2.23", "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078", "KEGG:K10563", "RefSeq:WP_010874736.1", "SO:0001217", "UniParc:UPI000012AB8D", "UniRef:UniRef100_P75402", "UniRef:UniRef50_P55825", "UniRef:UniRef90_P75402" ], "nt": "ATGCCTGAACTACCTGAAGTTGCCACTGTAATTACCGAACTTAAGAGCTGTGTTTTAAACAAACCTGTCAAGCAAGTTAAGGTGCACTTAGACAAGGTGTTGAAAAATACTAATGTCAAGCAGCTTAACGATGCGTTAGTCAACCACAGCTTTGTGGACATTAAGCGCCGCGGCAAGTACATTATTTTTTGTTTAAGTAACGGTTTGTTTTTAGTGTCACACTTACGCATGGAAGGCAAATATTTCTTTGAAGCTAAAGGCAGTCAGTTTGACTTAAACCATGTTTTAGTCGAGTTTTTATTCCAAGATGGTGATCAACTTAACTATCATGATACGCGTCAATTTGGTACCTTTCACTTGTTCAATAGATATCAGTTTGAAAACGCACGTGAATTGAACAAATTAGCACTCGATCCCTTAGATCAGGAATTTAACCATCAAGCGATCTTTAACAAAGGCCATAAGTCCAACAAGAAGATTAAAACCTTTATTTTAGATCAGACCAACATTAGCGGTATTGGCAATATTTACGCTGATGAAATTTTGTTTGCAAGTAAGATTCACCCTGAAACTTTAGCTAAAAATTTGAACTTATCGCAGTATCAGCTAATTTGTCAAAATGCAACTGATATTTTAAAGAAAGCAGTTGAAATGAAGGGTACTACCATTGGCACTTTTACCTTTAAAAAAGATCATACCGGTGGTTACCAACACTTCTTGAAAATTCATGGCAAAAAGGGGAAGCAATGTCAAAGCTGTAACACCACTATTATTAAGAAAAAGATTAATGGTCGGGGCAGCTATATTTGTGAAAAGTGTCAAATACAGCGTTAA", "aa": "MPELPEVATVITELKSCVLNKPVKQVKVHLDKVLKNTNVKQLNDALVNHSFVDIKRRGKYIIFCLSNGLFLVSHLRMEGKYFFEAKGSQFDLNHVLVEFLFQDGDQLNYHDTRQFGTFHLFNRYQFENARELNKLALDPLDQEFNHQAIFNKGHKSNKKIKTFILDQTNISGIGNIYADEILFASKIHPETLAKNLNLSQYQLICQNATDILKKAVEMKGTTIGTFTFKKDHTGGYQHFLKIHGKKGKQCQSCNTTIIKKKINGRGSYICEKCQIQR", "aa_hexdigest": "d964a67a07a0bb109692ac793c81463c", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI000012AB8D", "ncbi_nrp_id": "WP_010874736.1", "uniref100_id": "UniRef100_P75402", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012AB8D", "RefSeq:WP_010874736.1", "UniRef:UniRef100_P75402" ] }, "ips": { "uniref100_id": "UniRef100_P75402", "uniref90_id": "UniRef90_P75402" }, "psc": { "uniref90_id": "UniRef90_P75402", "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23" ], "uniref50_id": "UniRef50_P55825", "cog_id": "COG0266", "cog_category": "L", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003684", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078" ] }, "pscc": { "uniref50_id": "UniRef50_P55825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55825" ], "product": "Formamidopyrimidine-DNA glycosylase" }, "genes": [ "mutM" ], "id": "GIHJHMOALN_822", "locus": "GCA009939745_00800" }, { "type": "cds", "sequence": "contig_1", "start": 456456, "stop": 456659, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "GTGACTGGCCGTGATTACTGTCGCGTTCGTTGGTTAGAAAAGTACCTAAACACTGATCTACCAACAATTACCCTCGATGGCGCCATTATTTACTTCCGCGATGAAATTATTCGGCAAGAAGTGCTTGATAAGGAATTTATCCATGGCATTAACCAGATTGTCAAACGTTACCCTACAGCAGCCTTTAAGCTCAACATGGGTTGA", "aa": "MTGRDYCRVRWLEKYLNTDLPTITLDGAIIYFRDEIIRQEVLDKEFIHGINQIVKRYPTAAFKLNMG", "aa_hexdigest": "10eb145111f806d704660ca97303c7db", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 58, "aa_cov": 0.8507462686567164, "hmm_cov": 1.0, "evalue": 1.844487487960902e-07, "score": 26.570499420166016, "start": 2, "stop": 58 } ], "seq_stats": { "molecular_weight": 7964.164399999998, "isoelectric_point": 7.879986763000487 }, "id": "GIHJHMOALN_823", "locus": "GCA009939745_00801" }, { "type": "cds", "sequence": "contig_1", "start": 456789, "stop": 457037, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAAAGTAAATCAGGTTTACTTTGACACATTTACTTGCCCTGAACCAATGATCCAAGAGCTCGATAACTTAGTTGAAAAAAGTGATGTAACCGCCAAAAGCTACCGCCATTCACTTTACATTATTAAAAAAGGGGTATCGAAGGCTAGTGCACTACAAAACCTCCAACGAGACTTTTTAGTGGAAATGAAGCCTGCTAACACGATTGTCTTTGGTGATGGGGATAATGACATTGAAATGATGCAGTGA", "aa": "MKVNQVYFDTFTCPEPMIQELDNLVEKSDVTAKSYRHSLYIIKKGVSKASALQNLQRDFLVEMKPANTIVFGDGDNDIEMMQ", "aa_hexdigest": "39b9727459c323d7ef78bc4b7d0bf32e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 68, "aa_cov": 0.8292682926829268, "hmm_cov": 0.9705882352941176, "evalue": 4.00514977115385e-14, "score": 48.40382766723633, "start": 15, "stop": 82 } ], "seq_stats": { "molecular_weight": 9389.674399999998, "isoelectric_point": 4.93211269378662 }, "id": "GIHJHMOALN_824", "locus": "GCA009939745_00802" }, { "type": "cds", "sequence": "contig_1", "start": 457050, "stop": 457160, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCGTTAACTGGTAGTGATCCCGAGTGTTATAAGTTAGCTAAGTACCACACCGACAGCGTTGACGATGATGGGATTGCCAAATGGATTAATAAAAATCTGTTATGTTAA", "aa": "MSLTGSDPECYKLAKYHTDSVDDDGIAKWINKNLLC", "aa_hexdigest": "5b405e6f40582844bc0e46a4ed34cd66", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4045.5279999999993, "isoelectric_point": 4.822015571594237 }, "id": "GIHJHMOALN_825", "locus": "GCA009939745_00803" }, { "type": "cds", "sequence": "contig_1", "start": 457154, "stop": 457432, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01121.26" ], "nt": "ATGTTAATCGCTGTTGTAGGCAAAGCTGGAGTTGGTAAAACCACCGTTTTGCAATACATCGCTGACTATTTTCATTTTCCCGTTTTTTTTGCTGATCGGTTTATCCATCAACAATACGCAAATGGTCAAGCTGGTTATGCAATTGTAAAACAGCAATTTGGTGCTCAGTTTGTCAATCATGAAGCAGTTGATCGCAAACAGTTAGCACAATACGTTTTTAATCAACCTGATGAGTTAAAGCGTTTGTCCAACTTAACCAAACCCCTAGTACAAGAGTGA", "aa": "MLIAVVGKAGVGKTTVLQYIADYFHFPVFFADRFIHQQYANGQAGYAIVKQQFGAQFVNHEAVDRKQLAQYVFNQPDELKRLSNLTKPLVQE", "aa_hexdigest": "7443c091d42745058c1f67b8fe99bff9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01121.26", "name": "CoaE", "length": 92, "aa_cov": 0.9891304347826086, "hmm_cov": 0.9891304347826086, "evalue": 3.272145629744956e-14, "score": 48.453372955322266, "start": 1, "stop": 91 } ], "seq_stats": { "molecular_weight": 10475.864699999995, "isoelectric_point": 9.004637718200684 }, "id": "GIHJHMOALN_826", "locus": "GCA009939745_00804" }, { "type": "cds", "sequence": "contig_1", "start": 457686, "stop": 458534, "strand": "-", "frame": 2, "gene": "cof", "product": "Putative phosphatase MPN_383", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "KEGG:K21064", "RefSeq:WP_010874739.1", "SO:0001217", "UniParc:UPI0000139536", "UniRef:UniRef100_P75399", "UniRef:UniRef50_P75399", "UniRef:UniRef90_P75399" ], "nt": "ATGAAACCAAAAGTACAAAACTTAATTTTTGACCTCGATGGTACTTTATTGTCTTGGGGTCATGAACCACTACCACAAACTGTTACTTTTCTCAAAGAGTTGCAAAAGCAGGGGTTTAAAATTACCTTTGCGACTGGGCGCAGTCATATCTTAATCCGCAATACCACTCAGTTTATTCAACCTGATCTACCGGTTATTTCCTCTAACGGTGCTTTAATTTATGACTTTGCTCGGGAAAAAGCGCTGCATATGACCCAGTTAGCACCCCAAAGTGTGGTACCCATTATGCGTTTACTGTTGCAATTGGAAGAAAGCTTTTGCATTTACACTGATAAAAAGGTGTTTGGTTTTGAAAAACCCGGTATACCTTGCAAACGGTTACGCACTACTCAAAGCAAAATTGTAGAACCTGACATTACACAAAATAACTTTACCATTAATCCGTTAACTGATGCTAGCAAGTTTGATTTTGCCACACAAAACATTACCAAGATTCTTTTAATCACTGAAGACAGGGGTCGCATTAGTAAAATAACCAAACATCTCGATGCGATTGAAAACATTAGCTATGTCAGTTCTATGACCTTTGCGCTTGACATTATGCACAAAGATGTCAATAAGGCATACGGTCTCAAAGCATTAGAACAGCAAACCGGTTTAGATCCCCAAATGACCATGGTCTTTGGCGATGGGGACAATGATGTGGAAATTTTTAATGCTGTCAAGTACTCTGTCGCAATGGCTAACGGTAGTGATTTAGCAAAACAAAACGCGACCTTTATTTCGGAGTTTGATAATGACCATGATGGCATTTATCACTTTTTGCAATGCTTTCTAAAAATTGAGTAA", "aa": "MKPKVQNLIFDLDGTLLSWGHEPLPQTVTFLKELQKQGFKITFATGRSHILIRNTTQFIQPDLPVISSNGALIYDFAREKALHMTQLAPQSVVPIMRLLLQLEESFCIYTDKKVFGFEKPGIPCKRLRTTQSKIVEPDITQNNFTINPLTDASKFDFATQNITKILLITEDRGRISKITKHLDAIENISYVSSMTFALDIMHKDVNKAYGLKALEQQTGLDPQMTMVFGDGDNDVEIFNAVKYSVAMANGSDLAKQNATFISEFDNDHDGIYHFLQCFLKIE", "aa_hexdigest": "1ad92bee8a24b5640789e142d243a3bb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139536", "ncbi_nrp_id": "WP_010874739.1", "uniref100_id": "UniRef100_P75399", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139536", "RefSeq:WP_010874739.1", "UniRef:UniRef100_P75399" ] }, "ips": { "uniref100_id": "UniRef100_P75399", "uniref90_id": "UniRef90_P75399" }, "psc": { "uniref90_id": "UniRef90_P75399", "gene": "Cof", "product": "Putative phosphatase MPN_383", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P75399", "cog_id": "COG0561", "cog_category": "HR", "kegg_orthology_id": "K21064", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P75399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75399" ], "product": "Putative phosphatase MPN_383" }, "genes": [ "cof" ], "id": "GIHJHMOALN_827", "locus": "GCA009939745_00805" }, { "type": "cds", "sequence": "contig_1", "start": 458537, "stop": 459052, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "GTGCACAATTTCTTTCACAAGGAAAACATTATTAAAGAAACGGTAGATCAAGAAACTGTTTATGGTTATAACCTCTTTTTAAAGCGTAGTTACGAGCACTTAGAGAAACAAGAACTCAATTTGGTAATTAGCCAAATGATGATCTTTTTAAACCTGCTTTACAAAACAAAACAATTAACCCTGGCTTATGCAAAGGGCTTTTTGACGGTGTTATCGTTTTTTGCACCTTATTTAGCGGAAGAATTAAACGCTAAACTAGGGATGGAGCCGTTTATTGTTCATGCTCAACTACCAAATGTTGATAACAGCGTACTTGAAACCGATAAGGTTAAAATTATTCTTTCGGTCAACGGTCGCTTCAAAGGTACCAAGGAATTTGCTAAAGGGGTTGATGAACAGACTGTCTTAAAAGCCTTTAAGACAGATCCGGAATTCCAAGCGTTGTTCGACCAACCCTTAGCGCGTGTTGTTTTTGTCCCTAATCGTATTATTAATGTTCTTTTAAAATCCGAATAG", "aa": "MHNFFHKENIIKETVDQETVYGYNLFLKRSYEHLEKQELNLVISQMMIFLNLLYKTKQLTLAYAKGFLTVLSFFAPYLAEELNAKLGMEPFIVHAQLPNVDNSVLETDKVKIILSVNGRFKGTKEFAKGVDEQTVLKAFKTDPEFQALFDQPLARVVFVPNRIINVLLKSE", "aa_hexdigest": "e305be601141d12026cba9449c168731", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 143, "aa_cov": 0.672514619883041, "hmm_cov": 0.993006993006993, "evalue": 3.3575217738877222e-12, "score": 41.97935104370117, "start": 20, "stop": 134 } ], "seq_stats": { "molecular_weight": 19760.796199999993, "isoelectric_point": 6.921022605895996 }, "id": "GIHJHMOALN_828", "locus": "GCA009939745_00806" }, { "type": "cds", "sequence": "contig_1", "start": 459095, "stop": 459532, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGAAACAAGGTAATCAAACTTTTACTCGGGAAACGAGCACTATGCCCCAGTGGGCTGGTTCTTGTTGGTATTACTTGGGTTATTTGATGTTAATTAATAACGAAAACTTTTGGCCAATTGACTCCAAGGAAGCGAAAGATCTTTTTGAGCGTTACCTACCAGTGGATCTGTATGTGGGTGGTGCCGAACATGCCGTGTTGCACCTCTTGTACGCGCGCTTTTGGCACCAATTCTTGTACCAAAAGGGAATAGTCACCACTAAAGAGCCCTTTAAAAAGTTAATTAACCAGGGCATGGTATTGGGACCAGATGGCAAAAAGATGTCCAAATCCAAGGGCAACACAATCAACCCTACGCCACTAATTGATTCTCATGGTGCTGATGCCTTGCGACTTTACTTGATGTTTATGGGGCCGATTAGCGCGGCGTTGACCTGA", "aa": "MKQGNQTFTRETSTMPQWAGSCWYYLGYLMLINNENFWPIDSKEAKDLFERYLPVDLYVGGAEHAVLHLLYARFWHQFLYQKGIVTTKEPFKKLINQGMVLGPDGKKMSKSKGNTINPTPLIDSHGADALRLYLMFMGPISAALT", "aa_hexdigest": "fa35e8e3f6bbdb15f8ff6a03f6f758ff", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0012B0E3D9", "query_cov": 0.8551724137931035, "subject_cov": 0.9323308270676691, "identity": 0.621, "score": 157.0, "evalue": 7.79e-47, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A359BCU9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 138, "aa_cov": 0.9448275862068966, "hmm_cov": 0.8695652173913043, "evalue": 5.0178642286009785e-20, "score": 67.05760192871094, "start": 3, "stop": 139 } ], "seq_stats": { "molecular_weight": 16568.020999999982, "isoelectric_point": 8.946422767639163 }, "id": "GIHJHMOALN_829", "locus": "GCA009939745_00807" }, { "type": "cds", "sequence": "contig_1", "start": 459554, "stop": 460258, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF13603.12" ], "nt": "ATGCAAACCAACTGGATTGGTAAAAGTGAAGGGGTAGAGGTAACGTTTGACATAGAAAACTCCAAGGAAACGATTACCATTTTTACCACTAAGGTGCAAACTATTTTTGGTGTAACCTTTTTAGCGCTTTCCACTAGCCATCCCTTAGTAAAAGAGGTAGCTAAAACCAACCCCAAGATAGCACAGTTTCTCCAAAAACAAGCACAGAAAACCACCACTGTAAAGCAACCTGAGACGCTCCATGACGGGGTTGATTTAAAACTTAAAGCAATTAATCCAGCAACTAATACTGCCATTCCGTTGTATGTAGCTAACTATGTAGTGGAAGGTTATGGTACGGATGCTGTGATGGGTGTGCCAGCGCATAACGAAAACGATAACTTCTTCGCGCGGAAGCAAAAGCTACCGATTATTACCGTCATTGATAAACAGGAACGCTTGCAACACTCCGGGCAGTTTAGCGGGCTCAACTCCCAAACTGCTAACACCCAAATTACGCAAATGTTAGTCGAGCGTCAAAAAGCTAAAAAAACAACGGTGTACAAACTCCGTGACTGGATTTTTAGCCGTCAACGTTACTGGGGTGAACCTTTTCCGATTTTGTTTGACGAAAACAACCAACCCCATTTAGTTAAAGAGCTCCCCGTTACCCTACCAGCTTTGGCTAACTATCAACCTGATGGTTCGACTAACCCACCATTGTGA", "aa": "MQTNWIGKSEGVEVTFDIENSKETITIFTTKVQTIFGVTFLALSTSHPLVKEVAKTNPKIAQFLQKQAQKTTTVKQPETLHDGVDLKLKAINPATNTAIPLYVANYVVEGYGTDAVMGVPAHNENDNFFARKQKLPIITVIDKQERLQHSGQFSGLNSQTANTQITQMLVERQKAKKTTVYKLRDWIFSRQRYWGEPFPILFDENNQPHLVKELPVTLPALANYQPDGSTNPPL", "aa_hexdigest": "7b7a2d5b566bfd2bacbf2855331d8ea3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 39, "aa_cov": 0.16666666666666666, "hmm_cov": 1.0, "evalue": 1.4923236728469402e-07, "score": 25.971345901489258, "start": 170, "stop": 208 }, { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 180, "aa_cov": 0.6965811965811965, "hmm_cov": 1.0, "evalue": 1.204590925909463e-54, "score": 180.0886688232422, "start": 4, "stop": 166 } ], "seq_stats": { "molecular_weight": 26338.714400000004, "isoelectric_point": 8.942103385925293 }, "id": "GIHJHMOALN_830", "locus": "GCA009939745_00808" }, { "type": "cds", "sequence": "contig_1", "start": 460277, "stop": 460474, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGGAGATTGATGTCAACTGGTGTGCTGAATTGGGCACCGTTTTAGCCAACGAAGAGGTGTTGATTGACAGTAATGGTAATGCGGTGAGTGAGCGTGGCGAGTTCCCGGTAACCAAGCGAAAAATGAAGCAATGGGTGTTGAAAATAACTGCTTTTGCTGAAAGTTTACTCAATGGTTTGGCTGAGCTGGATTGA", "aa": "MKEIDVNWCAELGTVLANEEVLIDSNGNAVSERGEFPVTKRKMKQWVLKITAFAESLLNGLAELD", "aa_hexdigest": "2ab89cd3ebffe9460b2bc0c35556143d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7236.2179, "isoelectric_point": 4.67252902984619 }, "id": "GIHJHMOALN_831", "locus": "GCA009939745_00809" }, { "type": "cds", "sequence": "contig_1", "start": 460625, "stop": 460810, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTCCCTTATCCTTCAGCGGCTGGTTTACACCTGGGACATGTGCGCGCTTACACCATTACCGATGTTTTGAGTCGTTATCATAAAGCGAAGGGTTTTAATGTGATTCACCCGATTGGCTTTGATGCTTTTGGGTTACCAGCAGAACAATACGCGATTGCCTCCAATAAGCATCCTGGTGACTGA", "aa": "MFPYPSAAGLHLGHVRAYTITDVLSRYHKAKGFNVIHPIGFDAFGLPAEQYAIASNKHPGD", "aa_hexdigest": "f22d21b9d83767ebec682861ab446d36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 47, "aa_cov": 0.7540983606557377, "hmm_cov": 1.0, "evalue": 1.2426560071788628e-09, "score": 32.821102142333984, "start": 5, "stop": 50 } ], "seq_stats": { "molecular_weight": 6666.495200000001, "isoelectric_point": 8.155846977233889 }, "id": "GIHJHMOALN_832", "locus": "GCA009939745_00810" }, { "type": "cds", "sequence": "contig_1", "start": 460919, "stop": 461038, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCACTTCTTTATTCCAGTTGGTTTTGGTTTACTTTTTGGGATTGCTGTAGCCATTATAGCTGACCTGTTCTACCATTTAATTAAGTATTTAATTAAGCGCTCGTTCAAAAAAAACTAG", "aa": "MHFFIPVGFGLLFGIAVAIIADLFYHLIKYLIKRSFKKN", "aa_hexdigest": "ab688bbc77cdb5775449223d56b3321a", "start_type": "GTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4542.519700000001, "isoelectric_point": 9.997515296936037 }, "id": "GIHJHMOALN_833", "locus": "GCA009939745_00811" }, { "type": "cds", "sequence": "contig_1", "start": 461084, "stop": 461263, "strand": "-", "frame": 3, "gene": null, "product": "MPN385 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47509", "UniRef:UniRef90_P75397" ], "nt": "ATGCTGCTTAAACGGCTCATTAAACTAGCGATTTTCCTCTTCTTTGTCGCGGTTGGTTTTATTATTTTTATTGGCTCTTTTTGGCTCAATACCTACAATACTAAGGAGTGGGCTAATTTACTAGCTGAAAAGGATGCTAGTGGGCTAATTGTGCAAATTATTCCCAATATTAATCAATGA", "aa": "MLLKRLIKLAIFLFFVAVGFIIFIGSFWLNTYNTKEWANLLAEKDASGLIVQIIPNINQ", "aa_hexdigest": "9fb11d38dc51af3270ccbf0b7f3f43b8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 460911, "stop": 461263, "observations": { "deletion": [ 460927 ], "mutation": [ 461086 ], "stop": [ 461086 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75397", "query_cov": 1.0, "subject_cov": 0.5042735042735043, "identity": 1.0, "score": 114.0, "evalue": 8.39e-32, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MLLKRLIKLAIFLFFVAVGFIIFIGSFWLNTYNTKEWANLLAEKDASGLIVQIIPNINQWFKGTVEQQQKLFQTLVHFFIPVGFGLLFGIAVAIIADLFYHLIKYLIKRSFKKKLDV" }, "paralog": false, "identity": 0.9914529914529915, "score": 223.0, "evalue": 7.96e-77, "description": "Internal stop codon at 461086. Frameshift due to deletion around 460927. Nonsense mutation around 461086.", "psc": { "uniref90_id": "UniRef90_P75397", "product": "MPN385 family protein", "uniref50_id": "UniRef50_P47509", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75397", "UniRef:UniRef50_P47509", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47509", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47509" ], "product": "Uncharacterized protein MG267" } }, "id": "GIHJHMOALN_834", "locus": "GCA009939745_00812" }, { "type": "cds", "sequence": "contig_1", "start": 461298, "stop": 461666, "strand": "-", "frame": 2, "gene": "dck", "product": "putative MG268-like protein", "db_xrefs": [ "COG:COG1428", "COG:F", "GO:0005524", "GO:0005737", "GO:0019136", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_P75396" ], "nt": "TTGTTCGCCAAGCAAAACATCCACCGTCCTTCCATCTTTACTTACTATAACCACTTGTGGAATGGGATTGTTAAAGAACTAATCTTTAAACATGGTATTCCCGCTTTGTACGTTATCTTAGAAGGTGATTGGGAGTTGTTTGAACAAAGATTGTTTCAACGTAACCGTAAAGTAGAAATTGATAACTTTGCCAAAAATAAGGACTACTTTAAGAACCTGTACAAGATTTATGGTGAATTTATTAAAAACGTTTGTTATGACTTTGGTATTAGCCATTGCATAGTAAACGCTAACCAATCAGTAGAATCCATCACCAAACAAGTACTAGAGGTATTAAAGTCAAAAAACTTAGACTGGGAAATTATCTAG", "aa": "MFAKQNIHRPSIFTYYNHLWNGIVKELIFKHGIPALYVILEGDWELFEQRLFQRNRKVEIDNFAKNKDYFKNLYKIYGEFIKNVCYDFGISHCIVNANQSVESITKQVLEVLKSKNLDWEII", "aa_hexdigest": "a6fb0518e0e5df9168fc660f22ac9fea", "start_type": "TTG", "rbs_motif": null, "genes": [ "dck" ], "truncated": "3-prime", "pseudogene": { "start": 461298, "stop": 461966, "observations": { "mutation": [ 461669 ], "stop": [ 461669 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75396", "query_cov": 1.0, "subject_cov": 0.5327510917030568, "identity": 0.992, "score": 258.0, "evalue": 6.68e-86, "gene_start": 1, "gene_end": 122, "reference_start": 108, "reference_end": 229, "reference_sequence": "MKTPLKPKFQPAKIANAVVVGGMIAFGKTTIAESLAKHLKGSKVIYELEEQDQLADLLLAKMYERNDELLYAPLFQLYFTLNRFNKYRKECNNKTPTIFDRSIFEDWLFAKQNIHRPSIFTYYNHLWNGIVKELIFKHGIPALYVILEGDWELFEQRLFQRNRKVEIDNFAKNKDYFKNLYKIYGEFIKNVCYDFGISHCIVNANQSVESITKQVLEVLKSKNLDWEII" }, "paralog": false, "identity": 0.9954954954954955, "score": 449.0, "evalue": 1.02e-163, "description": "Internal stop codon at 461669. Nonsense mutation around 461669.", "psc": { "uniref90_id": "UniRef90_P75396", "gene": "Dck", "product": "Uncharacterized protein MG268 homolog", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0019136" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75396", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F", "GO:0005737", "GO:0005524", "GO:0019136" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "GIHJHMOALN_835", "locus": "GCA009939745_00813" }, { "type": "cds", "sequence": "contig_1", "start": 461667, "stop": 461987, "strand": "-", "frame": 2, "gene": "dck", "product": "putative MG268-like protein", "db_xrefs": [ "COG:COG1428", "COG:F", "GO:0005524", "GO:0005737", "GO:0019136", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_P75396" ], "nt": "ATGAAGACTCCCTTAAAACCCAAATTTCAACCAGCTAAGATTGCTAACGCCGTTGTGGTTGGTGGCATGATTGCTTTTGGTAAAACGACGATAGCTGAAAGTTTAGCTAAACACTTAAAGGGTTCTAAGGTGATTTACGAACTGGAAGAACAAGACCAGCTAGCTGATTTATTGTTAGCCAAAATGTATGAGCGTAACGACGAGTTGCTCTACGCCCCTTTATTTCAACTGTACTTTACCTTAAACCGCTTTAATAAATACCGCAAGGAATGTAACAATAAAACACCAACTATCTTTGACCGCTCGATCTTTGAAGATTGA", "aa": "MKTPLKPKFQPAKIANAVVVGGMIAFGKTTIAESLAKHLKGSKVIYELEEQDQLADLLLAKMYERNDELLYAPLFQLYFTLNRFNKYRKECNNKTPTIFDRSIFED", "aa_hexdigest": "0105850491dd30c47ea318e0d7109897", "start_type": "ATG", "rbs_motif": null, "genes": [ "dck" ], "truncated": "5-prime", "pseudogene": { "start": 461364, "stop": 461987, "observations": { "mutation": [ 461669 ], "stop": [ 461669 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75396", "query_cov": 1.0, "subject_cov": 0.462882096069869, "identity": 1.0, "score": 214.0, "evalue": 5.33e-69, "gene_start": 1, "gene_end": 106, "reference_start": 1, "reference_end": 106, "reference_sequence": "MKTPLKPKFQPAKIANAVVVGGMIAFGKTTIAESLAKHLKGSKVIYELEEQDQLADLLLAKMYERNDELLYAPLFQLYFTLNRFNKYRKECNNKTPTIFDRSIFEDWLFAKQNIHRPSIFTYYNHLWNGIVKELIFKHGIPALYVILEGDWELFEQRLFQRNRKVEIDNFAKNKDYFKNLYKIYGEFIKNVCYDFGISHCIVNANQSVESITKQVLEVLKSKNLDWEII" }, "paralog": false, "identity": 0.9951690821256038, "score": 423.0, "evalue": 1.78e-153, "description": "Internal stop codon at 461669. Nonsense mutation around 461669.", "psc": { "uniref90_id": "UniRef90_P75396", "gene": "Dck", "product": "Uncharacterized protein MG268 homolog", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0019136" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75396", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F", "GO:0005737", "GO:0005524", "GO:0019136" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "GIHJHMOALN_836", "locus": "GCA009939745_00814" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 462021, "stop": 462116, "strand": "-", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 60.8, "evalue": 1.1e-07, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "ATTTAGAGCCGTCACATCATTACGGTCGAATCATGTCAGGCCAGAAATGGAGCAGCATTAAGACTATTTAATGAGTGTGATGGTTTTTTATTGCAT", "id": "GIHJHMOALN_42", "locus": "GCA009939745_00815" }, { "type": "cds", "sequence": "contig_1", "start": 462135, "stop": 463211, "strand": "-", "frame": 2, "gene": null, "product": "putative MG269-like protein", "db_xrefs": [ "RefSeq:WP_010874743.1", "SO:0001217", "UniParc:UPI0000139546", "UniRef:UniRef100_P75395", "UniRef:UniRef50_Q49407", "UniRef:UniRef90_P75395" ], "nt": "ATGACTAACTTTGAGTATTACCGTGATTTTGATGACTTTCAAAGAAGAGAGACAATTAACTTCTTTCTTTCAAAGTTTCCACTAGCGAGTCAAAAACAGCTCCAGGACTTCCTAGAGCAAGCGCGTCAGGCTTATGTGCAGTTACGCCAAACCAACCCTGCACACCTTGACTGGAACCAAACATTGCTGTATTTAGCACAGAAATTTCTCCCCGAAAAGCAATCAGAGAAGGATCGCTTAAAGAAGATCTTGGTGTTGCAAGAACAGCTCAAAGTACGGTATGAAGGGGAAATTAAACGCCAGAGCCAACAAAACAGTGAGCTGTTAATCCAGTTAGGTCAACGTGATGAGGAAATTATCCAGATGCAGCAGCTATTTAAAGAAAAGGAACGGCAACTAGAGGTGTATCAAAAGCAGCTCAATGAAGCCAAGGAGTACAACCACAAGCTTGAGGAACACTACAACAAAACCCTGGAAGAAGCGTTGAAGGAGTACGAGCAGCAGTGTACTGATGCCATTCACAGGCGTGATGAGGAAATCCAGGCCATCTTTACAAGTAAGTTAAACGAAAAGAACAGTGAGATTACCCAACTGCAAACTTACTTGCAAAGTGCGGTTGATGAAAATGAGGCGTTGCAAAAGCAGCACAAACTAGTGTTGTTTAAAAACCAAAAGTACGAAAAGATGGTGAGTGACTTGCAAGTCGATTTAGCCCGGATTCAGGAAATTAACAATTCACTTACCAGTGAAAAGCGTGACTTTCAGCGCGCTAACAACGATTTGGTCAAGCAGTACAACAAACTCAAAAACCGCTTGGAACAAAAGTTAGGTGAAATTACTAACGCGCAAGTTAATGGTCAGACCCATACCGTTAGCCTAGCTGACACTTCACAGCAATTCCACCGTCCTCAAGAGGCTGTTATCCCGCAAACCCAAGTTATTAGCTATACACTCGATGATATGGATGATGACATGGAAGTGGAAGAAACTCCACCAACAACCAATAAAGATCTACCTCGCGGCGCGACCCAGCCGAAGCGTAATTCGATTAAGCGCGTAAGCAAATTAATTGATTAA", "aa": "MTNFEYYRDFDDFQRRETINFFLSKFPLASQKQLQDFLEQARQAYVQLRQTNPAHLDWNQTLLYLAQKFLPEKQSEKDRLKKILVLQEQLKVRYEGEIKRQSQQNSELLIQLGQRDEEIIQMQQLFKEKERQLEVYQKQLNEAKEYNHKLEEHYNKTLEEALKEYEQQCTDAIHRRDEEIQAIFTSKLNEKNSEITQLQTYLQSAVDENEALQKQHKLVLFKNQKYEKMVSDLQVDLARIQEINNSLTSEKRDFQRANNDLVKQYNKLKNRLEQKLGEITNAQVNGQTHTVSLADTSQQFHRPQEAVIPQTQVISYTLDDMDDDMEVEETPPTTNKDLPRGATQPKRNSIKRVSKLID", "aa_hexdigest": "e68b6232f2acd9e744b6f05bfa25e0e0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139546", "ncbi_nrp_id": "WP_010874743.1", "uniref100_id": "UniRef100_P75395", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139546", "RefSeq:WP_010874743.1", "UniRef:UniRef100_P75395" ] }, "ips": { "uniref100_id": "UniRef100_P75395", "uniref90_id": "UniRef90_P75395" }, "psc": { "uniref90_id": "UniRef90_P75395", "product": "Uncharacterized protein MG269 homolog", "uniref50_id": "UniRef50_Q49407" }, "pscc": { "uniref50_id": "UniRef50_Q49407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49407" ], "product": "Uncharacterized protein MG269" }, "genes": [], "id": "GIHJHMOALN_837", "locus": "GCA009939745_00816" }, { "type": "cds", "sequence": "contig_1", "start": 463204, "stop": 463590, "strand": "-", "frame": 1, "gene": null, "product": "putative MG269-1-like protein", "db_xrefs": [ "RefSeq:WP_014325503.1", "SO:0001217", "UniParc:UPI0001DD63CC", "UniRef:UniRef100_A0AAV5NAZ6", "UniRef:UniRef50_Q9EXD4", "UniRef:UniRef90_Q9EXD4" ], "nt": "ATGAGTAACTTCTTTGAGAAATACATTAACGGTTTTATTGAAACTTTAGACCAAATTGACGCAGCTGACTTTCAACGCATCCAACACGACTTTGACCCTAACCAGTTTCCGTATGACTGGGTGGTGGAACGGGTTAGTGATGTCAAGGATTATTTGCTCAATCCGCGTGATTTTAGCGATGTAGAAACTTTTAAAAGTACTATGCGTGCTAAGATTAAGCACTTTTATGCGTGTTATTCGAGCAAAATCCCCTTCTTTTTGTTTACTAGTTTTGTGTTAGCAATCTTTAACAGTGTTGGGCAGTATGTCAAGTACCACTGTGACTTGGACTTTACTAATCCTGATGCTGTAATTATCTTTTTCCGTGAAAAGGCCTTAAATGACTAA", "aa": "MSNFFEKYINGFIETLDQIDAADFQRIQHDFDPNQFPYDWVVERVSDVKDYLLNPRDFSDVETFKSTMRAKIKHFYACYSSKIPFFLFTSFVLAIFNSVGQYVKYHCDLDFTNPDAVIIFFREKALND", "aa_hexdigest": "89d7fb0eb7f125a51c13369f87c67d0e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63CC", "ncbi_nrp_id": "WP_014325503.1", "uniref100_id": "UniRef100_A0AAV5NAZ6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63CC", "RefSeq:WP_014325503.1", "UniRef:UniRef100_A0AAV5NAZ6" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NAZ6", "uniref90_id": "UniRef90_Q9EXD4" }, "psc": { "uniref90_id": "UniRef90_Q9EXD4", "product": "Uncharacterized protein MG269.1 homolog", "uniref50_id": "UniRef50_Q9EXD4" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD4" ], "product": "Uncharacterized protein MG269.1 homolog" }, "genes": [], "id": "GIHJHMOALN_838", "locus": "GCA009939745_00817" }, { "type": "cds", "sequence": "contig_1", "start": 463583, "stop": 463804, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10437.14" ], "nt": "GTGCAGGTAGATCAGGGTAAAGTAGTCGACATTGAGTTCTATGGGGACTTTTTGAGTGTTATCGACATTACCCCCGTTACCCAGCAGTTGGTAGGACAAAAATACGATTACCAAATCTTTGCTAAAATATTGGGTAACATTGATAACTTTAAGGAGTACTTTGGTACCTTAACACCTCAACAAATGCTCGAAGTTATCTTTGATAACAAAAAGGATGAGTAA", "aa": "MQVDQGKVVDIEFYGDFLSVIDITPVTQQLVGQKYDYQIFAKILGNIDNFKEYFGTLTPQQMLEVIFDNKKDE", "aa_hexdigest": "eb8f8748cca5a619ac10ea17491740ef", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10437.14", "name": "Lip_prot_lig_C", "length": 66, "aa_cov": 0.9041095890410958, "hmm_cov": 0.9848484848484849, "evalue": 1.2557079167400777e-16, "score": 55.89114761352539, "start": 1, "stop": 66 } ], "seq_stats": { "molecular_weight": 8483.539199999997, "isoelectric_point": 4.293867683410644 }, "id": "GIHJHMOALN_839", "locus": "GCA009939745_00818" }, { "type": "cds", "sequence": "contig_1", "start": 463880, "stop": 464602, "strand": "-", "frame": 3, "gene": "lplA", "product": "putative lipoate-protein ligase A", "db_xrefs": [ "COG:COG0095", "COG:H", "EC:6.3.1.20", "GO:0005524", "GO:0005737", "GO:0016979", "GO:0017118", "KEGG:K03800", "SO:0001217", "UniRef:UniRef50_P47512", "UniRef:UniRef90_P75394" ], "nt": "ATGAAAACTTACATTCTCACTTCCCCTAAAAACATTCCTTACTTCAACGCAGCGTTAGAAGAATGGCTACTCACCGAATTTAAAAAGGGCGAGGAAATTAAAGTCATTTACTTCTGGCAAAACGCCAACACGATTGTGGTGGGGCGCAACCAAAATACGTACGCTGAGGTTAACCTTAGTGAAGTGGAAAAGGACAAGGTTAATTTGTTTCGCCGTTTTTCTGGAGGTGGGACGGTGTTTCATGACATGGGTAACATCTGTTTCTCAATTATTTTGCCCAAAGCAAAAAAGGAAATGGAAAACGCTTATGAAGAAACAACCCGTAATGTGGTGAAGTTCTTAAACAGTGTAGGTGTACCCGCACAGTTTCATGGCCGCAATGACTTGGAAATTGAGGGCAAGAAGTTCTCTGGTTTAGCGGAATACCTCTCCAAAGACCGCGTGTTGGTCCACGGTACGTTGTTGTTTGATACCGACTTTACCAAGCTAGCGAAGTACCTCAATGTGGATAAAACAAAAATGGTAAGTAAGGGAATTGAAAGTGTCCAAAAACGGGTAGTTAACGTAAAAGAATACCTCCCCAACCTCTCTACCCCAACCTTCTTGGAAAAGATGGTGCAGTTCTTTACCGAAACCGAACATGCTGAAACGATTCATTTGGATGAAAGCAGCATTAAGATGGTGGAAAAACGTGCACAGGAACACTTCCAATCCTGGGATTGA", "aa": "MKTYILTSPKNIPYFNAALEEWLLTEFKKGEEIKVIYFWQNANTIVVGRNQNTYAEVNLSEVEKDKVNLFRRFSGGGTVFHDMGNICFSIILPKAKKEMENAYEETTRNVVKFLNSVGVPAQFHGRNDLEIEGKKFSGLAEYLSKDRVLVHGTLLFDTDFTKLAKYLNVDKTKMVSKGIESVQKRVVNVKEYLPNLSTPTFLEKMVQFFTETEHAETIHLDESSIKMVEKRAQEHFQSWD", "aa_hexdigest": "32f7335f3d99bc83074ce66df3c5ab47", "start_type": "ATG", "rbs_motif": null, "genes": [ "lplA" ], "truncated": "5-prime", "pseudogene": { "start": 463583, "stop": 464602, "observations": { "mutation": [ 463882 ], "stop": [ 463882 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75394", "query_cov": 1.0, "subject_cov": 0.7079646017699115, "identity": 0.996, "score": 477.0, "evalue": 1.25e-168, "gene_start": 1, "gene_end": 240, "reference_start": 1, "reference_end": 240, "reference_sequence": "MKTYILTSPKNIPYFNAALEEWLLTEFKKGEEIKVIYFWQNANTIVVGRNQNTYAEVNLSEVEKDKVNLFRRFSGGGAVFHDMGNICFSIILPKAKKEMENAYEETTRNVVKFLNSVGVPAQFHGRNDLEIEGKKFSGLAEYLSKDRVLVHGTLLFDTDFTKLAKYLNVDKTKMVSKGIESVQKRVVNVKEYLPNLSTPTFLEKMVQFFTETEHAETIHLDESSIKMVEKRAQEHFQSWDWNFGKTADYNFKNKKRFEGAGIFECNVQVDQGKVVDIKFYGDFLSVIDITPVTQQLVGQKYDYQTFAKILGNIDNFKEYFGTLTPQQMLEVIFDNKKDE" }, "paralog": false, "identity": 0.9882005899705014, "score": 676.0, "evalue": 2.33e-249, "description": "Internal stop codon at 463882. Nonsense mutation around 463882.", "psc": { "uniref90_id": "UniRef90_P75394", "gene": "lplA", "product": "Probable lipoate-protein ligase A", "ec_ids": [ "6.3.1.20" ], "uniref50_id": "UniRef50_P47512", "cog_id": "COG0095", "cog_category": "H", "kegg_orthology_id": "K03800", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016979", "GO:0017118" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75394", "EC:6.3.1.20", "UniRef:UniRef50_P47512", "COG:COG0095", "COG:H", "KEGG:K03800", "GO:0005737", "GO:0005524", "GO:0016979", "GO:0017118" ] }, "pscc": { "uniref50_id": "UniRef50_P47512", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47512" ], "product": "Probable lipoate-protein ligase A" } }, "id": "GIHJHMOALN_840", "locus": "GCA009939745_00819" }, { "type": "cds", "sequence": "contig_1", "start": 464629, "stop": 465759, "strand": "-", "frame": 1, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG1249", "EC:1.8.1.4", "GO:0004148", "GO:0005829", "GO:0006090", "GO:0006103", "GO:0016020", "GO:0050660", "KEGG:K00382", "SO:0001217", "UniRef:UniRef50_P75393", "UniRef:UniRef90_P75393" ], "nt": "TTGCTGGAGCAAAAAGGTAAGGTAGTGAGTAAGTTAGTTGGTGGGGTTAAAGCGATTATTGCTTCCGCTAAAGCTGAAACGGTAATGGGAGAAGCCAAGGTTTTGGATCCCAACACCGTGGAGGTAGCGGGTAAAACTTACACCACCAAAAGCATTGTGGTCGCAACTGGTTCACGACCCCGTTACTTAACGCTTCCGGGTTTTGCAGAAGCGCGTCAAAACGGTTTTGTAATTGACTCGACCCAAGCGCTCTCTTTGGAAGGGGTACCACGCAAGTTAGTTGTTGTTGGTGGTGGAGTGATTGGTATTGAATTTGCCTTTCTCTACGCTTCATTGGGTAGTGAAGTGACTATCTTACAAGGGGTGGATAGGATCTTGGAAATCTTTGACACTGAGGTATCAGATTTAGTCGCTAAACTCTTGCAAACCAAGAATGTCAAGATTATTACCAACGCACAGGTAACCCGTGCTAACAATAATGAAGTCTTTTATTCGCAAAACGGGCAGGAAGGCAGTGTGGTTGGTGATCGCATTTTGGTCTCGATTGGTCGCATTCCCAACACGGAATGTCTTGATGGGTTGAACTTACAACGTGATGAGCGCAACCGGATTGTGCTCAATCAAGATCTCCAAACCTCAATTCCCAACATCTACATAGTTGGCGATGCCAATGCCCAGTTAATGTTGGCCCACTTTGCCTACCAACAAGGGCGGTATGCGGTGAACCATATCTTAAACAAGAAGCAGGTCAAACCAGCGCAAAAGTTAACTTGCCCTTCCTGTATTTACACCAATCCGGAAGTAGCATCGGTGGGTTACACCGAAATGGAGTTGAAAAAACAGGGCATTCCTTATGTCAAAACCAATCTTGTTTTAGCGCACTGTGGAAAAGCAATCGCTGACAATGAAACCAACGGTTTTGTCAAAATGATGTTTGACCCACAAACCGGTAAGATCTTGGGTTGCTGTATTATTGCTGCTACTGCTAGTGACATGATTGCCGAGCTAGCGTTGGCTATGGATGCAGGTTTGACAGTATTTGACATTGCCAACTCCATTTCACCACATCCTACCATCAACGAAATGATCGCTGATGTGTGCAAAAAAGCTCTTTTTGATCACTTTAAATAA", "aa": "MLEQKGKVVSKLVGGVKAIIASAKAETVMGEAKVLDPNTVEVAGKTYTTKSIVVATGSRPRYLTLPGFAEARQNGFVIDSTQALSLEGVPRKLVVVGGGVIGIEFAFLYASLGSEVTILQGVDRILEIFDTEVSDLVAKLLQTKNVKIITNAQVTRANNNEVFYSQNGQEGSVVGDRILVSIGRIPNTECLDGLNLQRDERNRIVLNQDLQTSIPNIYIVGDANAQLMLAHFAYQQGRYAVNHILNKKQVKPAQKLTCPSCIYTNPEVASVGYTEMELKKQGIPYVKTNLVLAHCGKAIADNETNGFVKMMFDPQTGKILGCCIIAATASDMIAELALAMDAGLTVFDIANSISPHPTINEMIADVCKKALFDHFK", "aa_hexdigest": "b0024de008550d30ce99b54069ab5097", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75393", "query_cov": 1.0, "subject_cov": 0.8209606986899564, "identity": 0.995, "score": 723.0, "evalue": 1.11e-261, "valid": true, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "ec_ids": [ "1.8.1.4" ], "uniref50_id": "UniRef50_P75393", "cog_id": "COG1249", "cog_category": "C", "kegg_orthology_id": "K00382", "go_ids": [ "GO:0004148", "GO:0005829", "GO:0006090", "GO:0006103", "GO:0016020", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75393" ], "product": "Dihydrolipoyl dehydrogenase" }, "genes": [ "lpdA" ], "id": "GIHJHMOALN_841", "locus": "GCA009939745_00820" }, { "type": "cds", "sequence": "contig_1", "start": 465766, "stop": 466002, "strand": "-", "frame": 1, "gene": null, "product": "FAD-dependent oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "nt": "ATGAATTACGATCTCATTATTATAGGGGCTGGTCCCGCTGGGTATGTGGCCGCTGAGTACGCGGGTAAGCATAAACTTAAAACACTCGTTGTCGAAAAGGAATACTTTGGTGGCGTGTGTTTAAATGTTGGTTGTATTCCAACGAAAACACTGTTAAAACGGGCCAAGATTGTCGATTACCTCCGCCATGCGCAGGACTATGGTATTTCAATTAATGGTCAAGTAGCACTTAACTGA", "aa": "MNYDLIIIGAGPAGYVAAEYAGKHKLKTLVVEKEYFGGVCLNVGCIPTKTLLKRAKIVDYLRHAQDYGISINGQVALN", "aa_hexdigest": "c60591b31d3004a81de8c260af0c4654", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0010C3BE0E", "query_cov": 0.9358974358974359, "subject_cov": 0.9358974358974359, "identity": 0.589, "score": 100.0, "evalue": 1.85e-26, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A7X1ZRV2" }, "pscc": { "uniref50_id": "UniRef50_A0A7X1ZRV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "product": "FAD-dependent oxidoreductase" }, "genes": [], "id": "GIHJHMOALN_842", "locus": "GCA009939745_00821" }, { "type": "cds", "sequence": "contig_1", "start": 466003, "stop": 467211, "strand": "-", "frame": 1, "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "db_xrefs": [ "COG:C", "COG:COG0508", "EC:2.3.1.12", "GO:0004742", "GO:0005829", "GO:0009986", "GO:0016020", "GO:0031405", "RefSeq:WP_010874747.1", "SO:0001217", "UniParc:UPI0000130BFE", "UniRef:UniRef100_P75392", "UniRef:UniRef50_P75392", "UniRef:UniRef90_P75392" ], "nt": "ATGGCAAATGAGTTTAAGTTCACCGATGTTGGTGAGGGCTTACATGAGGGTAAAGTAACTGAAATCCTCAAAAAAGTGGGCGATACGATCAAGGTCGATGAAGCCCTTTTTGTAGTGGAAACCGATAAGGTAACCACCGAACTTCCATCCCCTTATGCTGGGGTTATTACCGCCATTACCACTAATGTTGGTGATGTGGTGCACATTGGTCAGGTGATGGCCGTTATTGATGATGGCGCTGGTGCTGCTGCACCTGCTGCGCCCCAACCAGTATCTGCACCAGCTCCTGCCCCAACCCCAACATTTACCCCAACACCAGCACCTGTAACCACCGAACCAGTAGTGGAAGAGGCGGGTGCCTCGGTAGTGGGTGAAATTAAGGTATCGAACAGTGTGTTTCCTATTTTTGGTGTGCAGCCAAGTGCGCCCCAACCAACACCAGCTCCTGTAGTACAACCTACATCCGCTCCAACTCCCACTCCAGCTCCAGCATCTGCAGCAGCACCTAGTGGTGAGGAAACGATTGCGATCACGACAATGCGTAAAGCAATTGCTGAAGCAATGGTGAAATCACACGAAAACATTCCCGCTACGATTCTCACCTTCTATGTTAACGCTACTAAGTTAAAGCAGTACCGTGAGAGTGTTAACGGCTTAGCTTTAAGCAAGTACAACATGAAGATTTCCTTCTTTGCTTTCTTTGTTAAAGCAATTGTTAATGCTTTGAAGAAGTTTCCTGTTTTCAATGGTCGTTATGACAAGGAACGCAACCTAATTGTGTTAAACAAGGATGTTAACGTAGGTATTGCCGTGGACACTCCCGATGGTCTGATTGTGCCTAACATTAAGCAAGCACAAACGAAGTCGGTAGTCGACATTGCCAAGGACATTGTTGATCTTGCTAACCGTGCCCGCAGTAAGCAAATTAAGCTTCCTGATTTAAGTAAGGGAACGATCTCGGTAACTAACTTTGGTTCTTTAGGCGCTGCTTTTGGTACCCCGATTATTAAGCACCCGGAAATGTGTATTGTCGCTACCGGTAACATGGAAGAACGTGTTGTGCGTGCTGAAGGTGGGGTAGCCGTTCATACCATCCTACCGTTAACAATTGCTGCCGACCACCGCTGGGTCGATGGCGCTGATGTGGGTCGTTTTGGTAAGGAAATTGCCAAGCAAATCGAAGAATTAATTGATTTAGAAGTAGCTTAG", "aa": "MANEFKFTDVGEGLHEGKVTEILKKVGDTIKVDEALFVVETDKVTTELPSPYAGVITAITTNVGDVVHIGQVMAVIDDGAGAAAPAAPQPVSAPAPAPTPTFTPTPAPVTTEPVVEEAGASVVGEIKVSNSVFPIFGVQPSAPQPTPAPVVQPTSAPTPTPAPASAAAPSGEETIAITTMRKAIAEAMVKSHENIPATILTFYVNATKLKQYRESVNGLALSKYNMKISFFAFFVKAIVNALKKFPVFNGRYDKERNLIVLNKDVNVGIAVDTPDGLIVPNIKQAQTKSVVDIAKDIVDLANRARSKQIKLPDLSKGTISVTNFGSLGAAFGTPIIKHPEMCIVATGNMEERVVRAEGGVAVHTILPLTIAADHRWVDGADVGRFGKEIAKQIEELIDLEVA", "aa_hexdigest": "eb234ec07be3b945730db8634bb71aab", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0000130BFE", "ncbi_nrp_id": "WP_010874747.1", "uniref100_id": "UniRef100_P75392", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000130BFE", "RefSeq:WP_010874747.1", "UniRef:UniRef100_P75392" ] }, "ips": { "uniref100_id": "UniRef100_P75392", "uniref90_id": "UniRef90_P75392" }, "psc": { "uniref90_id": "UniRef90_P75392", "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "ec_ids": [ "2.3.1.12" ], "uniref50_id": "UniRef50_P75392", "cog_id": "COG0508", "cog_category": "C", "go_ids": [ "GO:0009986", "GO:0005829", "GO:0016020", "GO:0004742", "GO:0031405" ] }, "pscc": { "uniref50_id": "UniRef50_P75392", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75392" ], "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex" }, "genes": [ "pdhC" ], "id": "GIHJHMOALN_843", "locus": "GCA009939745_00822" }, { "type": "cds", "sequence": "contig_1", "start": 467530, "stop": 467766, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02780.25" ], "nt": "GTGTTTAACTCCGTGAAGAAAACCGGGCGCTTGCTCGTAGTTACCGAAGCGGCTAAAACCTTTACCACCAGTGGGGAAATTATTGCATCGGTAACTGAAGAATTATTTAGCTATCTCAAGGCAGCCCCACAACGGGTAACCGGTTGGGACATTGTGGTACCGTTAGCACGGGGTGAACACTACCAGTTCAATTTAAATGCCCGCATTTTGGAAGCTGTTAACCAACTTTTAAAGTAA", "aa": "MFNSVKKTGRLLVVTEAAKTFTTSGEIIASVTEELFSYLKAAPQRVTGWDIVVPLARGEHYQFNLNARILEAVNQLLK", "aa_hexdigest": "42e4b34466ce553931a8307c3bb2b9f6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02780.25", "name": "Transketolase_C", "length": 60, "aa_cov": 0.7692307692307693, "hmm_cov": 1.0, "evalue": 1.5353895232584515e-13, "score": 46.22168731689453, "start": 3, "stop": 62 } ], "seq_stats": { "molecular_weight": 8694.967899999996, "isoelectric_point": 9.221831703186034 }, "id": "GIHJHMOALN_844", "locus": "GCA009939745_00823" }, { "type": "cds", "sequence": "contig_1", "start": 467779, "stop": 468513, "strand": "-", "frame": 1, "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "db_xrefs": [ "COG:C", "COG:COG0022", "EC:1.2.4.1", "GO:0001968", "GO:0004739", "GO:0005829", "GO:0009897", "GO:0009986", "GO:0016020", "GO:0031639", "GO:0033111", "GO:0051919", "SO:0001217", "UniRef:UniRef50_P75391", "UniRef:UniRef90_P75391" ], "nt": "ATGTCAAAAACAATTCAAGCAAATAACATTGAAGCGTTGGGTAACGCAATGGATCTAGCGTTAGAGCGCGACCCCAACGTTGTTTTATACGGTCAGGATGCTGGCTTTGAAGGTGGTGTGTTCCGTGCTACTAAGGGTTTACAAAAGAAGTACGGTGAGGAGCGTGTTTGGGACTGTCCGATTGCCGAAGCAGCTATGGCTGGTATTGGTGTGGGCGCTGCCATTGGTGGACTTAAACCAATCGTGGAAATCCAGTTCTCCGGTTTTTCTTTCCCCGCAATGTTCCAAATTTTTACCCACGCAGCACGGATCCGCAACCGTTCTCGCGGTGTGTATACCTGTCCGATTATTGTCCGCATGCCAATGGGTGGTGGGATTAAAGCACTTGAACACCACAGTGAGACCTTGGAAGCGATTTACGGTCAAATTGCCGGACTTAAAACAGTGATGCCGTCCAACCCTTACGACACCAAGGGCTTATTCTTAGCAGCAGTGGAATCACCTGACCCTGTGGTCTTCTTTGAACCAAAGAAGCTGTACCGTGCCTTCCGTCAGGAAATTCCAGCAGATTATTACACTGTGCCAATTGGTCAAGCTAACTTAATTAGCCAGGGTAACAACCTTACTATTGTTAGTTACGGTCCTACCATGTTTGACCTAATTAACATGGTCTATGGGGGTGAGTTGAAGGACAAGGGCATTGAACTCATTGACTTGCGTACCATTTCCCCTTGA", "aa": "MSKTIQANNIEALGNAMDLALERDPNVVLYGQDAGFEGGVFRATKGLQKKYGEERVWDCPIAEAAMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFTHAARIRNRSRGVYTCPIIVRMPMGGGIKALEHHSETLEAIYGQIAGLKTVMPSNPYDTKGLFLAAVESPDPVVFFEPKKLYRAFRQEIPADYYTVPIGQANLISQGNNLTIVSYGPTMFDLINMVYGGELKDKGIELIDLRTISP", "aa_hexdigest": "60542a124bde51fe8466edcf9360fd6d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FDA3C09", "query_cov": 0.930327868852459, "subject_cov": 0.9700854700854701, "identity": 0.555, "score": 267.0, "evalue": 3.08e-87, "valid": false, "product": "transketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A537VMS1" }, "genes": [ "pdhB" ], "truncated": "5-prime", "pseudogene": { "start": 467530, "stop": 468513, "observations": { "mutation": [ 467781 ], "stop": [ 467781 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75391", "query_cov": 1.0, "subject_cov": 0.746177370030581, "identity": 1.0, "score": 490.0, "evalue": 4.45e-174, "gene_start": 1, "gene_end": 244, "reference_start": 1, "reference_end": 244, "reference_sequence": "MSKTIQANNIEALGNAMDLALERDPNVVLYGQDAGFEGGVFRATKGLQKKYGEERVWDCPIAEAAMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFTHAARIRNRSRGVYTCPIIVRMPMGGGIKALEHHSETLEAIYGQIAGLKTVMPSNPYDTKGLFLAAVESPDPVVFFEPKKLYRAFRQEIPADYYTVPIGQANLISQGNNLTIVSYGPTMFDLINMVYGGELKDKGIELIDLRTISPWDKETVFNSVKKTGRLLVVTEAAKTFTTSGEIIASVTEELFSYLKAAPQRVTGWDIVVPLARGEHYQFNLNARILEAVNQLLK" }, "paralog": false, "identity": 0.9969418960244648, "score": 646.0, "evalue": 9.28e-238, "description": "Internal stop codon at 467781. Nonsense mutation around 467781.", "psc": { "uniref90_id": "UniRef90_P75391", "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "ec_ids": [ "1.2.4.1" ], "uniref50_id": "UniRef50_P75391", "cog_id": "COG0022", "cog_category": "C", "go_ids": [ "GO:0033111", "GO:0009986", "GO:0005829", "GO:0009897", "GO:0016020", "GO:0001968", "GO:0004739", "GO:0031639", "GO:0051919" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75391", "EC:1.2.4.1", "UniRef:UniRef50_P75391", "COG:COG0022", "COG:C", "GO:0033111", "GO:0009986", "GO:0005829", "GO:0009897", "GO:0016020", "GO:0001968", "GO:0004739", "GO:0031639", "GO:0051919" ] }, "pscc": { "uniref50_id": "UniRef50_P75391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75391" ], "product": "Pyruvate dehydrogenase E1 component subunit beta" } }, "id": "GIHJHMOALN_845", "locus": "GCA009939745_00824" }, { "type": "cds", "sequence": "contig_1", "start": 468535, "stop": 468771, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAGTGACCCTGTGAAGCGGTTACGTAACTTCTTGTTTGACCGTGGTATTCTAACCCCACAACAAGAAGAGGAAATGGTAGCTAAGATTGAACAAGAAGTGCAAGCTGCATATGAGGTAATGGTTAGCAAAACGCCAGTTACCTTGGATGAAGTGTTTGATTACAACTATGAAAAGTTGACTCCTGATCTAGCGCGCCAAAAGGCTGAAGCTAAAAAATACTTTAAAGACTAA", "aa": "MKSDPVKRLRNFLFDRGILTPQQEEEMVAKIEQEVQAAYEVMVSKTPVTLDEVFDYNYEKLTPDLARQKAEAKKYFKD", "aa_hexdigest": "22a018bb5e0a188eee3497f3ddf07ced", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9202.413899999992, "isoelectric_point": 5.180442237854003 }, "id": "GIHJHMOALN_846", "locus": "GCA009939745_00825" }, { "type": "cds", "sequence": "contig_1", "start": 469060, "stop": 469170, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTGCACTACAAGAAACTCCCTAACGTAGCCGTTACGATGATTGGGGATGGTGGTACAGCTGAAGGTGAGTTTTATGAAGCGATGAACATTGCTTCCATTCACAAGTGA", "aa": "MLHYKKLPNVAVTMIGDGGTAEGEFYEAMNIASIHK", "aa_hexdigest": "cd628000bc1b3f624d4cf663fe100233", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 34, "aa_cov": 0.9444444444444444, "hmm_cov": 1.0, "evalue": 1.8965348561940097e-09, "score": 32.36134338378906, "start": 3, "stop": 36 } ], "seq_stats": { "molecular_weight": 3937.5207, "isoelectric_point": 5.9887495040893555 }, "id": "GIHJHMOALN_847", "locus": "GCA009939745_00826" }, { "type": "cds", "sequence": "contig_1", "start": 469273, "stop": 469425, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTGAACTTTGCGCCTAACTTAGGTGAGGAAGCACTGCAAGTTGGTATGGGCATGGGTTTAAATGAAAATGACTGGTTCTGCCCTACCTTCCGTTCCGGTGCGTTAATGTTGTACCGTGGTGTTAAACCAGAACAGCTTTTACTTTACTGA", "aa": "MLNFAPNLGEEALQVGMGMGLNENDWFCPTFRSGALMLYRGVKPEQLLLY", "aa_hexdigest": "fdbb75e2166b67bf059591c6d23abef4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 45, "aa_cov": 0.9, "hmm_cov": 1.0, "evalue": 7.473533470686637e-08, "score": 27.122459411621094, "start": 5, "stop": 49 } ], "seq_stats": { "molecular_weight": 5636.5244999999995, "isoelectric_point": 4.584314918518066 }, "id": "GIHJHMOALN_848", "locus": "GCA009939745_00827" }, { "type": "cds", "sequence": "contig_1", "start": 469441, "stop": 469611, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTTTGATTAAAAACAAAGTCCCTACTACACTTTACCAAGTGTATGACAATGAGGGTAAGTTAATGGACCCTAATCACAAGATTACACTTAGCAACGAACAGTTAAAGCACGCTTTTTACCTCATGAATCTTTCCCGAATCATGGACAAGAAGATGCTTGTTTGA", "aa": "MAILIKNKVPTTLYQVYDNEGKLMDPNHKITLSNEQLKHAFYLMNLSRIMDKKMLV", "aa_hexdigest": "5bab2ac35b6accac1ac55ef5124ba67c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6594.8087, "isoelectric_point": 9.398539543151855 }, "id": "GIHJHMOALN_849", "locus": "GCA009939745_00828" }, { "type": "cds", "sequence": "contig_1", "start": 469632, "stop": 469760, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGATCAGTGAACTGGGTTTAGTAGACGTTTACTTTCTTCCCCACTACAATAAGCCGTTCAACTTTGTGTTAGCAGCCGTGTTGCAAGCGCTTGGTTTTAGTTACTATACCCCTAAAAATAAATAA", "aa": "MLISELGLVDVYFLPHYNKPFNFVLAAVLQALGFSYYTPKNK", "aa_hexdigest": "445622aaa8dfa37c456841e475e603d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4822.619100000001, "isoelectric_point": 8.085060691833494 }, "id": "GIHJHMOALN_850", "locus": "GCA009939745_00829" }, { "type": "cds", "sequence": "contig_1", "start": 469815, "stop": 470480, "strand": "-", "frame": 2, "gene": "nox", "product": "NADH oxidase", "db_xrefs": [ "COG:COG0446", "COG:I", "EC:1.6.3.4", "GO:0008137", "SO:0001217", "UniRef:UniRef50_Q49408", "UniRef:UniRef90_P75389" ], "nt": "ATGTTGGACAAACCAGCTGGCAATAACTTTGATGAAGAGTTTACCAACGAGTTGGAAAAAGCCATGAAGAAAGCGGGCATTAACCTAATGATGGGTAGTGCCGTTAAGGGCTTTATTGTTGATGCGGACAAAAATGTCGTTAAAGGTGTTGAAACCGATAAGGGTCGGGTGGATGCTGACCTTGTCATCCAATCGATTGGGTTCCGCCCAAACACCCAGTTTGTCCCTAAAGACAGGCAGTTTGAGTTTAACCGTAACGGTTCGATTAAGGTTAATGAATACCTCCAAGCCTTAAATCATGAAAATGTGTATGTTATTGGTGGGGCTGCTGCCATTTATGATGCAGCTAGTGAACAATACGAAAACATTGACTTAGCTACCAACGCTGTGAAAAGTGGTTTAGTCGCTGCTATGCACATGATTGGTAGCAAGGCTGTAAAGTTAGAGTCCATTGTTGGTACCAATGCGCTGCACGTATTTGGTTTAAATCTAGCAGCTACTGGTTTAACCGAAAAACGTGCCAAAATGAACGGTTTTGATGTGGGAGTGAGCATTGTTGATGACAACGACCGTCCCGAATTTATGGGGACCTTTGACAAGGTGCGCTTTAAGTTAATTTACGACAAGAAGACGCTCCGTTTGTTGGGTGCCCAATTACTTTCGTGA", "aa": "MLDKPAGNNFDEEFTNELEKAMKKAGINLMMGSAVKGFIVDADKNVVKGVETDKGRVDADLVIQSIGFRPNTQFVPKDRQFEFNRNGSIKVNEYLQALNHENVYVIGGAAAIYDAASEQYENIDLATNAVKSGLVAAMHMIGSKAVKLESIVGTNALHVFGLNLAATGLTEKRAKMNGFDVGVSIVDDNDRPEFMGTFDKVRFKLIYDKKTLRLLGAQLLS", "aa_hexdigest": "3f23ecc26d2f5074f2ab9cc1410e1184", "start_type": "ATG", "rbs_motif": null, "genes": [ "nox" ], "truncated": "both", "pseudogene": { "start": 469632, "stop": 470780, "observations": { "mutation": [ 469817, 470513, 470729 ], "stop": [ 469817, 470513, 470729 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75389", "query_cov": 1.0, "subject_cov": 0.4613778705636743, "identity": 1.0, "score": 429.0, "evalue": 7.4e-148, "gene_start": 1, "gene_end": 221, "reference_start": 198, "reference_end": 418, "reference_sequence": "MKKVIVIGVNHAGTSFIRTLLSKSKDFQVNAYDRNTNISFLGCGIALAVSGVVKNTEDLFYSTPEELKAMGANVFMAHDVVGLDLDKKQVIVKDLATGKETVDHYDQLVVASGAWPICMNVENEVTHTQLQFNHTDKYCGNIKNLISCKLYQHALTLIDSFRHDKSIKSVAIVGSGYIGLELAEAAWQCGKQVTVIDMLDKPAGNNFDEEFTNELEKAMKKAGINLMMGSAVKGFIVDADKNVVKGVETDKGRVDADLVIQSIGFRPNTQFVPKDRQFEFNRNGSIKVNEYLQALNHENVYVIGGAAAIYDAASEQYENIDLATNAVKSGLVAAMHMIGSKAVKLESIVGTNALHVFGLNLAATGLTEKRAKMNGFDVGVSIVDDNDRPEFMGTFDKVRFKLIYDKKTLRLLGAQLLSWNTNHSEIIFYIALAVQKKMLISELGLVDVYFLPHYNKPFNFVLAAVLQALGFSYYTPKNK" }, "paralog": false, "identity": 0.9921465968586387, "score": 746.0, "evalue": 3.92e-275, "description": "Internal stop codon at 469817, 470513, 470729. Nonsense mutation around 469817, 470513, 470729.", "psc": { "uniref90_id": "UniRef90_P75389", "gene": "nox", "product": "NADH oxidase", "ec_ids": [ "1.6.3.4" ], "uniref50_id": "UniRef50_Q49408", "cog_id": "COG0446", "cog_category": "I", "go_ids": [ "GO:0008137" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75389", "EC:1.6.3.4", "UniRef:UniRef50_Q49408", "COG:COG0446", "COG:I", "GO:0008137" ] }, "pscc": { "uniref50_id": "UniRef50_Q49408", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49408" ], "product": "NADH oxidase" } }, "id": "GIHJHMOALN_851", "locus": "GCA009939745_00830" }, { "type": "cds", "sequence": "contig_1", "start": 470511, "stop": 470717, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGTGGAAAACGAAGTAACCCACACCCAATTGCAATTCAACCACACTGATAAGTACTGTGGCAATATTAAGAACTTAATTAGTTGTAAGTTGTACCAACACGCCCTTACCTTAATTGACAGCTTCCGTCATGACAAGTCAATTAAATCAGTAGCCATTGTGGGCTCGGGTTACATTGGTTTAGAGTTAGCAGAAGCGGCTTGA", "aa": "MNVENEVTHTQLQFNHTDKYCGNIKNLISCKLYQHALTLIDSFRHDKSIKSVAIVGSGYIGLELAEAA", "aa_hexdigest": "2dc24f838a546b751a95cb4b1fe21983", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7592.557099999999, "isoelectric_point": 6.380313301086425 }, "id": "GIHJHMOALN_852", "locus": "GCA009939745_00831" }, { "type": "cds", "sequence": "contig_1", "start": 470727, "stop": 471071, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07992.20" ], "nt": "ATGAAGAAAGTGATTGTTATCGGTGTCAATCACGCCGGCACCAGTTTTATCAGAACCCTTTTAAGCAAGAGCAAAGACTTTCAGGTCAACGCTTATGATCGCAATACCAACATCTCCTTTTTGGGTTGTGGAATTGCCTTGGCAGTTAGTGGTGTAGTTAAAAACACCGAAGACCTGTTCTACTCCACCCCTGAAGAGCTAAAAGCTATGGGTGCTAATGTCTTTATGGCTCATGATGTAGTTGGTCTTGATCTAGACAAAAAACAAGTAATCGTGAAGGACTTGGCCACTGGTAAGGAAACTGTGGACCACTATGATCAATTGGTGGTAGCCTCTGGAGCTTGA", "aa": "MKKVIVIGVNHAGTSFIRTLLSKSKDFQVNAYDRNTNISFLGCGIALAVSGVVKNTEDLFYSTPEELKAMGANVFMAHDVVGLDLDKKQVIVKDLATGKETVDHYDQLVVASGA", "aa_hexdigest": "5b3d93c88d0a16c4b7e8d15b26fa8f75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07992.20", "name": "Pyr_redox_2", "length": 115, "aa_cov": 0.9912280701754386, "hmm_cov": 0.9478260869565217, "evalue": 2.993339449342785e-16, "score": 55.137691497802734, "start": 2, "stop": 114 } ], "seq_stats": { "molecular_weight": 12303.003799999991, "isoelectric_point": 6.256517982482911 }, "id": "GIHJHMOALN_853", "locus": "GCA009939745_00832" }, { "type": "cds", "sequence": "contig_1", "start": 471139, "stop": 471672, "strand": "-", "frame": 1, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209", "KEGG:K00759", "RefSeq:WP_054173813.1", "SO:0001217", "UniParc:UPI0006BA42EF", "UniRef:UniRef100_UPI0006BA42EF", "UniRef:UniRef50_P75388", "UniRef:UniRef90_P75388" ], "nt": "GTGAAACAGAAACTCCAAGCGCTTGATCGAGCTATTAAGCGATTTAATGACTTTCCCACTCCGGGAATCCTCTTTTACGACATTACGCCAATTTTTTTAAACAGTGAACTGTTTGAGTTTGTACTGGAACAAATGGCACAGTTTATCCAGGAAGTTAAAGCTGATGGCATCGTATGTCCCGAAGCGCGTGGTTTTATCTTTGGTGGTGCTTTGGCTTCCAAAACCAAACTACCCCTAGTGTTAGTGCGCAAACCCCATAAGCTATCAGGTGAGTTAGCGCGTGAAACCTATGATTTGGAATACCGCCAAAACTCAATCTTGGAAATGCGGGTTGATGCACTAGAAAACTACAAGCGCTGTGTGATTGTTGATGACTTACTAGCTACTGCTGGTACGGTTGCAGCGATTGACAAACTAATAGCGCGTTTAGGTAGTCAAACTGTGGGTTATTGTTTTTTAATTGAACTGCAAAAACTGCACGGTAAAGCAAAGCTACAACCAAATGTGGCAACCAAGATCTTGCTCCACTATTAA", "aa": "MKQKLQALDRAIKRFNDFPTPGILFYDITPIFLNSELFEFVLEQMAQFIQEVKADGIVCPEARGFIFGGALASKTKLPLVLVRKPHKLSGELARETYDLEYRQNSILEMRVDALENYKRCVIVDDLLATAGTVAAIDKLIARLGSQTVGYCFLIELQKLHGKAKLQPNVATKILLHY", "aa_hexdigest": "6b0902ba3fc69f0aa98b2046342546d8", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0006BA42EF", "ncbi_nrp_id": "WP_054173813.1", "uniref100_id": "UniRef100_UPI0006BA42EF", "db_xrefs": [ "SO:0001217", "UniParc:UPI0006BA42EF", "RefSeq:WP_054173813.1", "UniRef:UniRef100_UPI0006BA42EF" ] }, "ips": { "uniref100_id": "UniRef100_UPI0006BA42EF", "uniref90_id": "UniRef90_P75388" }, "psc": { "uniref90_id": "UniRef90_P75388", "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_P75388", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_P75388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75388" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "GIHJHMOALN_854", "locus": "GCA009939745_00833" }, { "type": "cds", "sequence": "contig_1", "start": 471707, "stop": 471958, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTACGCTGCCTTTAACTTTGGTGGTATTAACGGCACGATTAACCTAGTGATCTTCTACTTAATTGCGATTGTGGCCTTGTTTAGCGTGTTTGTCAATGGCTTACCGCTGTTTAGCTTTGGTTTAATCAATGGTTGGTTAAGTCCGTACAACTATGTCCAAATCCACATTACCTTACGCCACAAAAAGCAAATGTTTAAGGAAGTGGATCAAATTGAAGAACAGTTGATTAGTGGAATTAACAGCTTTTAA", "aa": "MYAAFNFGGINGTINLVIFYLIAIVALFSVFVNGLPLFSFGLINGWLSPYNYVQIHITLRHKKQMFKEVDQIEEQLISGINSF", "aa_hexdigest": "fc43ab3fc82cab266e16351a1a6b4022", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9445.953199999996, "isoelectric_point": 6.702078819274903 }, "id": "GIHJHMOALN_855", "locus": "GCA009939745_00834" }, { "type": "cds", "sequence": "contig_1", "start": 472004, "stop": 472555, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACCAAAACATCGAGTAACTACTCGATCTTAACGGAAAACAACGACATCCAAACGGACTTCTTTATCAGTAAGAACCAGCACGACTTCTTTGGTAAGAAAAAGTGGAGCTTGCTGTTTATTTGGTTGACAGTTCTCGCCTTTGGGGTGGTGATGCTCGGCCTTTATCTGGGCGCACCACAACTGTTAGGTAACTTTTTAGCCGCTGACATCGAATCGAGTACTGGCATTATGTTAGGTGTGGGGGTGATTAGCTTGCTCTATCTCTTGTATTCGCTACCGCGCTATGGTGTCGCTTATGGGATTAGCTACCTAATTGCTTTAATTCTGTTAGCCGCTGGACTTTTTGGGGCAATGTACCTCGCTAACTTTATCTTTAGGATTGACCAGTCCACAATTCAACTAATTATCTTTGTGTACCTGTTCTGGCTGTTCTTCCAAGCCCGCATTGGTCAAACTACCATTTGGACTTACTGTTACTGGTTTAAACGCAGCTTGAAGGACCGGGTCTTTGTGAAAAACCTCTTTAACGACAATGTTAACAGCCAATGA", "aa": "MTKTSSNYSILTENNDIQTDFFISKNQHDFFGKKKWSLLFIWLTVLAFGVVMLGLYLGAPQLLGNFLAADIESSTGIMLGVGVISLLYLLYSLPRYGVAYGISYLIALILLAAGLFGAMYLANFIFRIDQSTIQLIIFVYLFWLFFQARIGQTTIWTYCYWFKRSLKDRVFVKNLFNDNVNSQ", "aa_hexdigest": "76a70d4e574f6633854a977e17852675", "start_type": "TTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20991.397200000003, "isoelectric_point": 9.161811637878419 }, "id": "GIHJHMOALN_856", "locus": "GCA009939745_00835" }, { "type": "cds", "sequence": "contig_1", "start": 472589, "stop": 472771, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGAAACCCACATTTGCTGGTTATTGAGTGCCTTGGTCGTGATTTACCTCTCCAACTACCAGGTACAACAGTTAGGTACCTTAATGGCAGTTTCAGCGATTACCTCTTACTTCTTAAACTATGGTTTAGGAGTAATCTTAGTGTGGTTGTTTGTGTCATCCCATTCCGGGTTAGAATGA", "aa": "MIETHICWLLSALVVIYLSNYQVQQLGTLMAVSAITSYFLNYGLGVILVWLFVSSHSGLE", "aa_hexdigest": "ac302e42dfe3dbde8c9e7e7eca00c1a4", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6706.840099999998, "isoelectric_point": 5.364145851135253 }, "id": "GIHJHMOALN_857", "locus": "GCA009939745_00836" }, { "type": "cds", "sequence": "contig_1", "start": 472802, "stop": 473731, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF44FC6", "UniRef:UniRef90_UPI000AF44FC6" ], "nt": "GTGCGCAACATCTTTAACGTTCCTAAAGGTGGTACTGATTACCTCACCTTTAACGAACGTGAAAAGAACCTCTGGGACTTCTTGCACGCTGAGGGTAACTTTTCCTCTGGTGATAATTTGTTTTTGCAAAAAAATAATAATGGTAATTCACCAATTACCAAAGCTAGTGACATTACCTTAAAAAATATCTATTACATCTATGCAGCACCGACACATTTCTCACAAGTTGCAACCAAAGACAATAACAATAAGGACGCTGCTGATGTTGTCACTGTAGCTAACAGCGCGGACACTAGAAAACTGCGCAGCGGTGATGCTACTGGTTTTTCTGGTTTATTCTCCAATTACATCTTGGCAGAAATTAGAACAGAAGATCCGGTAGCTAAGAATGGTGATCCGGTTAAGGTAAACGCCACTTTACAAAGCTTTTTGGATAGCAATAACCAACGCTTAGAACACGGTGGTAACATTAAGTTCCAAGTAGCTAACTTTGTTAACAATGATGGTTCATACGTTCCTGCTTCTTACTTGGAAGCTAGTAGGGTAAAGGTGCTGTTGAGCAATGGTTTTCCCGAAACCGCTACCATTGCAGCTGCTAACACCAAGTTAGTTAATGCACCGCTTTCCAACACTTTAGCACGCGATGTTTCTAACTTTGCTTCGAGCTTCATTGCCTTGGGTGTAATTATCCTGCTCGCTACAATTGCGTTAGGTATTCGTTACAAGCTACTTGGCCTTTACAAAGCCTTAGCCTTAGCGCTTAGTGCCATCTCTTCGCTCGCCTTCTTTAATGCCGTTGGTGGTGTCGTTGATGTCTTTTCGTTTGTCGGCATCTTCTTTGTGGTTGCCGTTAACATTATTAACCTGATCACTCTGGCGGAACTGTTTTTGCGTAACATTAAGAACAACGCCTCAATTGTGGAATCGTGA", "aa": "MRNIFNVPKGGTDYLTFNEREKNLWDFLHAEGNFSSGDNLFLQKNNNGNSPITKASDITLKNIYYIYAAPTHFSQVATKDNNNKDAADVVTVANSADTRKLRSGDATGFSGLFSNYILAEIRTEDPVAKNGDPVKVNATLQSFLDSNNQRLEHGGNIKFQVANFVNNDGSYVPASYLEASRVKVLLSNGFPETATIAAANTKLVNAPLSNTLARDVSNFASSFIALGVIILLATIALGIRYKLLGLYKALALALSAISSLAFFNAVGGVVDVFSFVGIFFVVAVNIINLITLAELFLRNIKNNASIVES", "aa_hexdigest": "a7530daa11969610a7f7d29b90b59a08", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 472499, "stop": 473902, "observations": { "mutation": [ 472591, 472804, 473818 ], "stop": [ 472591, 472804, 473818 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF44FC6", "query_cov": 1.0, "subject_cov": 0.42270861833105333, "identity": 0.997, "score": 576.0, "evalue": 1.17e-200, "gene_start": 1, "gene_end": 309, "reference_start": 58, "reference_end": 366, "reference_sequence": "MDFIKSKSLTDSDYNTGNGQKSVEGSGKWFKDKANGSAQNGNKTLVLWKDKEGAVQYVRNIFNVPKGGTDYLTFNEREKNLWDFLHAEGNFSSGDNLFLQKNNNGNSPITKASDITLKNIYYIYAAPTHFSQVATKDNNNKDAADVVTVANSADTRKLRSGDATGFSGLFSNYILAEIRTEDPVAKNGDPVKVNATLQSFLDSNNQRLEHGGNIKFQVANFVNNDGSYVPASYLEASRVKVLLSNGFPETATIAAANTKLVNAPLSNTLARDVSNFASSFIALGVIILLATIALGIRYKLLGLYKALALALSAISSLAFFNAVGGVVDVFSFVGIFFVVAVNIINLITLAELFLRNIKNNASIVESWKLCLKRSFFTMIETHICWLLSALVVIYLSNYQVQQLGTLMAVSAITSYFLNYGLGVILVWLFVSSHSGLEWRFFLYKKDAQALTKTSSNYSILTENNDIQTDFFISKNQHDFFGKKKWSLLFIWLTVLAFGVVMLGLYLGAPQLLGNFLAADIESSTGIMLGVGVISLLYLLYSLPRYGVAYGISYLIALILLAAGLFGAMYLANFIFRIDQSTIQLIIFVYLFWLFFQARIGQTTIWTYCYWFKRSLKDRVFVKNLFNDNVNSQWRLDLIGSSVLILLFIVYAAFNFGGINGTINLVIFYLIAIVALFSVFVNGLPLFSFGLINGWLSPYNYVQIHITLRHKKQMFKEVDQIEEQLISGINSF" }, "paralog": false, "identity": 0.9914346895074947, "score": 900.0, "evalue": 0.0, "description": "Internal stop codon at 472591, 472804, 473818. Nonsense mutation around 472591, 472804, 473818.", "psc": { "uniref90_id": "UniRef90_UPI000AF44FC6", "uniref50_id": "UniRef50_UPI000AF44FC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF44FC6", "UniRef:UniRef50_UPI000AF44FC6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF44FC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF44FC6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 33403.45650000003, "isoelectric_point": 7.841628074645997 }, "id": "GIHJHMOALN_858", "locus": "GCA009939745_00837" }, { "type": "cds", "sequence": "contig_1", "start": 474215, "stop": 474622, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACTGAAAAAGCGGGTTTCCTTTGACTGGCTCTTAAAGATTGGAATTATCCTGGTCTTAGCTTTTTTGGGCCTGTTTGGAATTATTTTTGGTAGTTATAAGCTTCTAAACGACAGTCGCTTAGGGGTAGTGTTCAACGGTTCCACTACCACCACTGTTTATTTTTTAAACCATAAGTCCAAGGACGCTAACAAGGAGTTAGATCCTACCCAGAAAAAGGACGGCAATGGCAACGGTAACAATACTGAAATGATTACCGATGTGAACGGTTTTCTCAACAATATTGAAAAGAGTTACGCCAACAGCCTCTACGCCCAGGGTTTTTCGTCTGTTAACATCACGAAAAACTCACACGATACAAGTGCAAAAGAACTTTCAGATAAAGGAGTTTTTGACAGTTCTTGA", "aa": "MGLKKRVSFDWLLKIGIILVLAFLGLFGIIFGSYKLLNDSRLGVVFNGSTTTTVYFLNHKSKDANKELDPTQKKDGNGNGNNTEMITDVNGFLNNIEKSYANSLYAQGFSSVNITKNSHDTSAKELSDKGVFDSS", "aa_hexdigest": "7b13d6e5c114d116c04d20bbdbc27d57", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14821.56319999998, "isoelectric_point": 8.803883171081544 }, "id": "GIHJHMOALN_859", "locus": "GCA009939745_00838" }, { "type": "cds", "sequence": "contig_1", "start": 474665, "stop": 474775, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCACTTTAGTTGAAATTGAACGCGATTTTTTACAAAAAACAGCCCAAAAATTTGCACCTGAAGTGGTTGCGTTGATTACTAAAGCACTCGATTACTCCAAAAAGTGA", "aa": "MATLVEIERDFLQKTAQKFAPEVVALITKALDYSKK", "aa_hexdigest": "ddd94cdd00635392bc3c5ea8e08e66f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4096.786800000001, "isoelectric_point": 8.099243736267091 }, "id": "GIHJHMOALN_860", "locus": "GCA009939745_00839" }, { "type": "cds", "sequence": "contig_1", "start": 474857, "stop": 476320, "strand": "+", "frame": 2, "gene": "spoT", "product": "putative guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "db_xrefs": [ "COG:COG0317", "COG:TK", "EC:3.1.7.2", "GO:0005886", "GO:0008893", "GO:0015970", "SO:0001217", "UniRef:UniRef50_P75386", "UniRef:UniRef90_P75386" ], "nt": "ATGGACAGCAACACCGTGTGTGCTGGTCTGTTACACGACATTATTGAAGATACCCAGGTCACTGAAGCTGATTTAACTGCCATTTTTGGTAAAGAAATTACTGATTTAGTGGTAAAAGTTACCAAAATTACCAGTGAATCCAAAAAACAACGCCAGCTAAACCGAAAGAAAGAAGATTTAAACCTCAAAAGTTTGGTTAACATTGCGATGAGCTCGCAGCAAGAGGTCAATGCTTTGGTGTTAAAGTTGGCCGACCGACTCGACAACATCTCCTCAATTGAATTTTTAGCAGTCGAAAAACAAAAGATTATTGCTAAAGAAACACTCGAACTGTATGCCAAGATAGCCGGTCGAATTGGAATGTATCCCGTTAAAACTCAACTAGCCGATCTTTCCTTTAAGGTATTAGATCCAAAAAACTTTAACAATACGCTGTCCAAGATTAACCAGCAAAAGGTCTTTTATGACAACGAATGGGACAACTTTAAAAAACAGTTGGAAGAGATGCTGGAACAAAACCAGATTGAATACCGCTTAGAAAGTCGAATTAAAGGCATTTATTCTACTTATCAAAAATTAACCTTTCACGAGCAGAACATTGCCAAAATCCACGATCTGTTCGCCATTCGTTTAATTGTGAAGTCAGAGTTGGATTGTTACCACTTACTTGGTTTAATCCACCTCAACTTTACCGTGTTAATGAAACACTTTAAGGATTACATAGCATCCCCCAAACAAAACTTTTACCAATCAATCCACACGACTGTGCGCTTGAAGGGTTTAAATGTGGAAATCCAGATTAGAACCCAACGAATGGATCATGTGAGCAAGTACGGTTTTGCTAGCCACTGGATTTACAAAGAGAAAAAGGAAGGGTTATTGGCAAGTGCTTTACAAGTAAATTACTTAAACAGTAAACAAATGCATTCGCGCGATTTTTTTAAACGAATCTTTGGTACTGATATTATTAAGGTTAATGTAAGTTCCGATAACGAACCTAACATAGTCAAAAAACTCAATGTTGAAAGTAACAGCAAGTTGCTCGATATTGCTTATGAGTTGTATCCCAAACAGTTCAATAAATTAGAAAAAATCAAACTCGATGGGGTTGAGGTTATGTCGTTTGACGTAACTGCCGAAAACGAAATGGTAATTGAGTTTTGTTTTGGTAAAACCAATAACCTCAAACGGCGTTGGTTACGTTATATGAATAACCCCGTATTTCGCGAACGGGTTAAAAAGGATTTAAACAAGTTAAAGAAAGCGGTTAAGTACAGTGAACTACCTCTATACGAAAAGGCCTTGGAAGAGTTGCACTTAAAGCTAGCTGATGAAACCCAGATTAAACAACGCTTAAATGCTTTAGGGATCAAAAAACTGACAGAGTTCTTGGAATTAATCGAATACCCCCACTTCCCTAAAAATGAGCATTTGTACTTTTTAGCTAGTAACAATCAGAAGTGA", "aa": "MDSNTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSESKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKLADRLDNISSIEFLAVEKQKIIAKETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWDNFKKQLEEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLIHLNFTVLMKHFKDYIASPKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIYKEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDIIKVNVSSDNEPNIVKKLNVESNSKLLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYMNNPVFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLELIEYPHFPKNEHLYFLASNNQK", "aa_hexdigest": "7d78d64061dc10d15ea814f4569315b2", "start_type": "ATG", "rbs_motif": null, "genes": [ "spoT" ], "truncated": "both", "pseudogene": { "start": 474626, "stop": 476623, "observations": { "mutation": [ 474638, 474773, 474851, 476318, 476558 ], "stop": [ 474638, 474773, 474851, 476318, 476558 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75386", "query_cov": 1.0, "subject_cov": 0.6643929058663028, "identity": 0.996, "score": 933.0, "evalue": 0.0, "gene_start": 1, "gene_end": 487, "reference_start": 78, "reference_end": 564, "reference_sequence": "MFYNWLKLYKFSKMATLVEIERDFLQKTAQKFAPEVVALITKALDYSKKWHGEQKRLSGEPFFIHPLRTALRLVEWNMDSNTVCAGLLHDIIEDTQVTEADLTAIFGKEITDLVVKVTKITSESKKQRQLNRKKEDLNLKSLVNIAMSSQQEVNALVLKLADRLDNISSIEFLAVEKQKIIAKETLELYAKIAGRIGMYPVKTQLADLSFKVLDPKNFNNTLSKINQQKVFYDNEWGNFKKQLEEMLEQNQIEYRLESRIKGIYSTYQKLTFHEQNIAKIHDLFAIRLIVKSELDCYHLLGLIHLNFTVLMKHFKDYIASPKQNFYQSIHTTVRLKGLNVEIQIRTQRMDHVSKYGFASHWIYKEKKEGLLASALQVNYLNSKQMHSRDFFKRIFGTDIIKVNVSSDNEPNIVKKLNVESNSKLLDIAYELYPKQFNKLEKIKLDGVEVMSFDVTAENEMVIEFCFGKTNNLKRRWLRYMNNHVFRERVKKDLNKLKKAVKYSELPLYEKALEELHLKLADETQIKQRLNALGIKKLTEFLELIEYPHFPKNEHLYFLASNNQKWRELIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCTKIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKIIPLYWNAHQLKMRPRKFRCQINIRGVWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQITTKNISYVWKYL" }, "paralog": false, "identity": 0.9894736842105263, "score": 1289.0, "evalue": 0.0, "description": "Internal stop codon at 474638, 474773, 474851, 476318, 476558. Nonsense mutation around 474638, 474773, 474851, 476318, 476558.", "psc": { "uniref90_id": "UniRef90_P75386", "gene": "spoT", "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "ec_ids": [ "3.1.7.2" ], "uniref50_id": "UniRef50_P75386", "cog_id": "COG0317", "cog_category": "TK", "go_ids": [ "GO:0005886", "GO:0008893", "GO:0015970" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75386", "EC:3.1.7.2", "UniRef:UniRef50_P75386", "COG:COG0317", "COG:TK", "GO:0005886", "GO:0008893", "GO:0015970" ] }, "pscc": { "uniref50_id": "UniRef50_P75386", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75386" ], "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase" } }, "id": "GIHJHMOALN_861", "locus": "GCA009939745_00840" }, { "type": "cds", "sequence": "contig_1", "start": 476327, "stop": 476560, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAACCCATTAAGTTCGCTTTATCACAAGCTGTGTTCCAAAACTCTTACTTTGAACAAATTGAGGGAATTTACATCACCAAAATTGTGATTGAAACCTGTTGCACAAAGATTCCTGACATGCCAGAACAAGTTATTGGCATCTTAATGAAAAACATTCTCAGGGTACACCTTCATGATTGTAGGGAATTAGCTAATCAAAAGCAACCCAAAATTATTCCACTTTATTGA", "aa": "MIKPIKFALSQAVFQNSYFEQIEGIYITKIVIETCCTKIPDMPEQVIGILMKNILRVHLHDCRELANQKQPKIIPLY", "aa_hexdigest": "694f63dd98f8263921d95be02114cff8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8959.653899999994, "isoelectric_point": 8.504556465148926 }, "id": "GIHJHMOALN_862", "locus": "GCA009939745_00841" }, { "type": "cds", "sequence": "contig_1", "start": 476579, "stop": 476815, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGACCACGTAAATTCCGCTGTCAAATTAACATTCGCGGTGTATGGAGTGAAACAACGGTCAATAAAATTGTTCAAACCATTATTGAAGGTGATAGCTATTTAGAACGAATTATTCCCAAGATCGATAAACAAAAAGATGAGTTTGAGCTCAACATTACTATGTTTATCGACAACTATCACCAACTAATCACAATTATGGAGCAGATTACTACTAAGAATATTAGCTACGTTTGA", "aa": "MRPRKFRCQINIRGVWSETTVNKIVQTIIEGDSYLERIIPKIDKQKDEFELNITMFIDNYHQLITIMEQITTKNISYV", "aa_hexdigest": "ed0906eba75f04ab6eb63e460804548d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9362.786699999993, "isoelectric_point": 7.868124580383301 }, "id": "GIHJHMOALN_863", "locus": "GCA009939745_00842" }, { "type": "cds", "sequence": "contig_1", "start": 476824, "stop": 477480, "strand": "-", "frame": 1, "gene": null, "product": "putative MG279-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014325509.1", "SO:0001217", "UniParc:UPI0001DD63D2", "UniRef:UniRef100_A0AAV5N8B0", "UniRef:UniRef50_P47521", "UniRef:UniRef90_P75385" ], "nt": "ATGTTTAAGTTTTTAAAAAAGCTTTCCACTTTCCTAATTGTTTTGATTGGTATTCTACTTGTTGGTGGCATTACAGCAGCGGGTTACTTTGCCTTTGAAAACCGTGAACCTATTAATAACTATTACAAGGAAGGTTATAACAAGGTTAAACAATATAACGAGGAAATTAAAAAAGTTTCAAAGTCACTTTCTAGTAACGAACTTGTAAAAACGTTAGGTGATGTTGAAAGTTCAATTAAAGAAGGAAAAGAATTGACAAAATTACTGGATGACAGTGCTTTAGAAAGCAGTTTTAACCAACTCGAAGATTCATTAAGCAAGGTTAACAACTTCTCAAAAGGATCTACTTTTACTGAAGTTAAGAACACAATTGAAAAGATTAATCAATACGTGGACGAAATCTTAAAACGTTTTCCAAATCCTAACGAAAATGATCAGTTCAAGGAATACGTAACTAATATTTCACAAATTGTCTTTTATGTCGGGGTATCTATTATTGGTACATTTGTTGTTTCTGGTGCCTTATTGTTTATTTTCACCAAACGCGTTTATGGGGTACGAGTATCGCGCTTTAACCCACAAAGGCTATTGAAGAAACACTTAGTGTTACTTTTACAAAAGAACCAAGATGTTTATGACGAAGTATTTGAAAGTTAA", "aa": "MFKFLKKLSTFLIVLIGILLVGGITAAGYFAFENREPINNYYKEGYNKVKQYNEEIKKVSKSLSSNELVKTLGDVESSIKEGKELTKLLDDSALESSFNQLEDSLSKVNNFSKGSTFTEVKNTIEKINQYVDEILKRFPNPNENDQFKEYVTNISQIVFYVGVSIIGTFVVSGALLFIFTKRVYGVRVSRFNPQRLLKKHLVLLLQKNQDVYDEVFES", "aa_hexdigest": "8195bf9a6b05dd97d88947838b5de441", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63D2", "ncbi_nrp_id": "WP_014325509.1", "uniref100_id": "UniRef100_A0AAV5N8B0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63D2", "RefSeq:WP_014325509.1", "UniRef:UniRef100_A0AAV5N8B0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N8B0", "uniref90_id": "UniRef90_P75385" }, "psc": { "uniref90_id": "UniRef90_P75385", "product": "Uncharacterized protein MG279 homolog", "uniref50_id": "UniRef50_P47521", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47521", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47521" ], "product": "Uncharacterized protein MG279" }, "genes": [], "id": "GIHJHMOALN_864", "locus": "GCA009939745_00843" }, { "type": "cds", "sequence": "contig_1", "start": 477480, "stop": 478343, "strand": "-", "frame": 2, "gene": null, "product": "putative MG280-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014325510.1", "SO:0001217", "UniParc:UPI0001DD63D3", "UniRef:UniRef100_A0AAV5NB22", "UniRef:UniRef50_P75384", "UniRef:UniRef90_P75384" ], "nt": "ATGCTCCTTTTTATTAATCGCTTTGCCAAAACAATCATTCTGTTCTTCGGGATGCTTGTCTTTTTGGTTTTGTTAGGTCTTGGCGGTGCTGCACTTTACTTTAAAGACAACGCAGCTAAACTTTACATTGATACCCGCAAATCGATCGACAGTTCCTTTGACTCTTCTCAAGCGTTTATAGATACTTATAATGGAAGTTCCAGTAAGTTTAGTGTTGAATCCATTAATAAACAAATAGAAGAGGTTAAGAAGAAAGTTGAAGAATCAACTAAAAAGTTAGAGGAGTACGAAAAGCAAATAAACCAAGCTAAAGGCTTAAACGGCTATCTTGTGAGTCCGGAAAAACTAAAGGAGTTACAAGAAGCTAAGAAGAGTCTACAAGCTACTAAGAGTCAAATAGAAAAGTATGCAAATACTTTAAAAACCGCTAATAATGGTAAAACTGGTCAAAATGGCACATCTTCTTCGACTATTCCAATTACTAAGATTTCTGGTTCAACAATTAGTGTAAGTACCAGAGATACTAACGGTAAAACCAATAGTGCTTTGAAAGATATTCAAGAGTTCTCTACACAAGCAAATGACATCATTAAACAGTACAAAGAAATTAAAAACAAAATTCCAACAGAAAAACAGTTTAATGAATATTACACCATAGGCGCAATTACCTTAGTTAGTGTTTCTGGTGGCGTTTTAGCAGTGCTAATAGTCTCTACTGTAATGACCTTCTTAGGTTCGAAAAAGTTAGGACTTAGAACGTTTTCGCGTTTAACCAGTACTGATCAAATTGCTGATCATGTTAATGACATCTTAGATCGTTATCCAGAGTTGGAAGATGCTGTTTTAGAGGAGCTTGATCAATAA", "aa": "MLLFINRFAKTIILFFGMLVFLVLLGLGGAALYFKDNAAKLYIDTRKSIDSSFDSSQAFIDTYNGSSSKFSVESINKQIEEVKKKVEESTKKLEEYEKQINQAKGLNGYLVSPEKLKELQEAKKSLQATKSQIEKYANTLKTANNGKTGQNGTSSSTIPITKISGSTISVSTRDTNGKTNSALKDIQEFSTQANDIIKQYKEIKNKIPTEKQFNEYYTIGAITLVSVSGGVLAVLIVSTVMTFLGSKKLGLRTFSRLTSTDQIADHVNDILDRYPELEDAVLEELDQ", "aa_hexdigest": "0342094c0c3ceb97afe2967795ea16bc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63D3", "ncbi_nrp_id": "WP_014325510.1", "uniref100_id": "UniRef100_A0AAV5NB22", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63D3", "RefSeq:WP_014325510.1", "UniRef:UniRef100_A0AAV5NB22" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NB22", "uniref90_id": "UniRef90_P75384" }, "psc": { "uniref90_id": "UniRef90_P75384", "product": "Uncharacterized protein MG280 homolog", "uniref50_id": "UniRef50_P75384", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75384", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75384" ], "product": "Uncharacterized protein MG280 homolog" }, "genes": [], "id": "GIHJHMOALN_865", "locus": "GCA009939745_00844" }, { "type": "cds", "sequence": "contig_1", "start": 478405, "stop": 478911, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22806.1", "PFAM:PF20757.2", "PFAM:PF22805.1" ], "nt": "GTGAAAAACACTAATGAAAAAAATGTCAATAAAGACAACGACACCGTTTACGCTGCTCTAAAAGACATTAATCTCCACTTAGAGGAAACTTACACTCATGGCGGCAACACGATGTACCGGGTGAACGAAAATTACTATCCGGGTGCCAGTGCTTACGAAGCGGAACGCGCTACCCGTGATAGTGAGTTCCAAAAGGAAATTGTACAACGCGCTGAACTTATTGGGGTTGTCTTTGAATATGGAGTGAAAAACCTCCGTCCGGGTTTAAAGTACACCGTGAAGTTTGAAAGTCCACAAGAACAAGTGGCATTAAAGAGTACCGATAAGTTCCAACCAGTTATTGGTAGTGTCACTGATATGAGCAAATCAGTTACTGATTTAATCGGTGTATTACGTGATAACGCCGAAATTCTCAATATTACTAATGTTTCTAAAGATGAAACCGTTGTAGCGGAATTGAAAGAAAAATTGGATCGGGAAAATGTTTTCCAAGAGATTCGAACTTAA", "aa": "MKNTNEKNVNKDNDTVYAALKDINLHLEETYTHGGNTMYRVNENYYPGASAYEAERATRDSEFQKEIVQRAELIGVVFEYGVKNLRPGLKYTVKFESPQEQVALKSTDKFQPVIGSVTDMSKSVTDLIGVLRDNAEILNITNVSKDETVVAELKEKLDRENVFQEIRT", "aa_hexdigest": "0390aac5cde1d52b2939ce76a7f62944", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22806.1", "name": "M_C", "length": 99, "aa_cov": 0.5833333333333334, "hmm_cov": 1.0, "evalue": 9.84714300112677e-54, "score": 175.40232849121094, "start": 71, "stop": 168 }, { "id": "PF20757.2", "name": "M_large_dom", "length": 42, "aa_cov": 0.25, "hmm_cov": 1.0, "evalue": 3.660250907861363e-19, "score": 64.40087890625, "start": 1, "stop": 42 }, { "id": "PF22805.1", "name": "M_smaller_dom", "length": 28, "aa_cov": 0.16666666666666666, "hmm_cov": 1.0, "evalue": 1.244294847572403e-22, "score": 74.97817993164062, "start": 43, "stop": 70 } ], "seq_stats": { "molecular_weight": 19127.170200000015, "isoelectric_point": 5.087453651428221 }, "id": "GIHJHMOALN_866", "locus": "GCA009939745_00845" }, { "type": "cds", "sequence": "contig_1", "start": 478939, "stop": 479781, "strand": "-", "frame": 1, "gene": null, "product": "IgG-blocking protein M", "db_xrefs": [ "GO:0005515", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47523", "UniRef:UniRef90_P75383" ], "nt": "GTGCCTAAGGTAGAGGTGAGCGGTTTGATTAAAACCAGTTCTACCTTTATCACGCCAAACTTTAAAGCGGGTTACTACGACCAAGTAGCAGCGGACGGGAAAACGCTCAAATATTACCAATCAACCGAATACTTTAACAACCGGGTAGTAATGCCAATTTTGCAAACTACCAACGGCACTTTAACTGCTAACAATAGGGCTTACGATGATATCTTTGTCGATCAAGGTGTCCCTAAGTTCCCTGGTTGGTTCCATGACGTGGATAAGGCTTACTATGCTGGCTCCAATGGACAAAGCGAGTACTTGTTTAAAGAGTGGAATTACTATGTTGCTAACGGTTCACCGTTATACAACGTTTACCCAAACCATCACTTTAAGCAAATAAAAACGATTGCCTTTGATGCTCCAAGGATTAAGCAAGGTAATACTGACGGCATTAACCTCAATTTAAAACAACGTAATCCTGATTACGTCATCATTAATGGTTTAACTGGTGATGGTTCTACCTTAAAGGATTTGGAATTACCAGAAAGCGTTAAAAAGGTGTCAATTTATGGTGACTACCACTCGATTAATGTTGCTAAGCAAATTTTTAAAAACGTGTTAGAGTTGGAGTTTTACTCTACTAACCAAGACAATAACTTTGGTTTTAATCCACTAGTTTTAGGCGATCACACTAATATTATCTATGACCTTTTTGCTTCCAAGCCGTTTAATTACATTGATTTAACTAGTTTAGAACTTAAAGATAACCAAGACAATATTGATGCATCTAAGTTAAAGCGTGCTGTTAGTGACATTTACATTCGTCGTCGTTTTGAACGACAAATGCAAGGTTACTGA", "aa": "MPKVEVSGLIKTSSTFITPNFKAGYYDQVAADGKTLKYYQSTEYFNNRVVMPILQTTNGTLTANNRAYDDIFVDQGVPKFPGWFHDVDKAYYAGSNGQSEYLFKEWNYYVANGSPLYNVYPNHHFKQIKTIAFDAPRIKQGNTDGINLNLKQRNPDYVIINGLTGDGSTLKDLELPESVKKVSIYGDYHSINVAKQIFKNVLELEFYSTNQDNNFGFNPLVLGDHTNIIYDLFASKPFNYIDLTSLELKDNQDNIDASKLKRAVSDIYIRRRFERQMQGY", "aa_hexdigest": "94e1cc0758c17af4793dcbe08767d015", "start_type": "GTG", "rbs_motif": "AAAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 478636, "stop": 480081, "observations": { "mutation": [ 478941 ], "stop": [ 478941 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75383", "query_cov": 1.0, "subject_cov": 0.48109965635738833, "identity": 0.996, "score": 570.0, "evalue": 6.89e-201, "gene_start": 1, "gene_end": 280, "reference_start": 125, "reference_end": 404, "reference_sequence": "MKLNFKIKDKKTLKRLKKGGFWALGLFGAAINAFSAVLIVNEVLRLQSGETLIASGRSGNLSFQLYSKVNQNAKSKLNSISLTDGGYRSEIDLGDGSNFREDFRNFANNLSEAITDAPKDLLRPVPKVEVSGLIKTSSTFITPNFKAGYYDQVAADGKTLKYYQSTEYFNNRVVMPILQTTNGTLTANNRAYDDIFVDQGVPKFPGWFHDVDKAYYAGSNGQSEYLFKEWNYYVANGSPLYNVYPNHHFKQIKTIAFDAPRIKQGNTDGINLNLKQRNPDYVIINGLTGDGSTLKDLELPESVKKVSIYGDYHSINVAKQIFKNVLELEFYSTNQDNNFGFNPLVLGDHTNIIYDLFASKPFNYIDLTSLELKDNQDNIDASKLKRAVSDIYIRRRFERQMQGYWAGGYIDRYLVKNTNEKNVNKDNDTVYAALKDINLHLEETYTHGGNTMYRVNENYYPGASAYEAERATRDSEFQKEIVQRAELIGVVFEYGVKNLRPGLKYTVKFESPQEQVALKSTDKFQPVIGSVTDMSKSVTDLIGVLRDNAEILNITNVSKDETVVAELKEKLDRENVFQEIRT" }, "paralog": false, "identity": 0.997920997920998, "score": 968.0, "evalue": 0.0, "description": "Internal stop codon at 478941. Nonsense mutation around 478941.", "psc": { "uniref90_id": "UniRef90_P75383", "product": "IgG-blocking protein M", "uniref50_id": "UniRef50_P47523", "go_ids": [ "GO:0005515", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75383", "UniRef:UniRef50_P47523", "GO:0005515", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47523" ], "product": "Uncharacterized protein MG281" } }, "id": "GIHJHMOALN_867", "locus": "GCA009939745_00846" }, { "type": "tRNA", "sequence": "contig_1", "start": 480441, "stop": 480514, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 74.9, "nt": "GCGAGTATAGTTTAGTGGTAGAACATCAGCCTTCCAAGCTGATCGTGTCGGTTCGATTCCGATTACTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 480480, 480482 ], "id": "GIHJHMOALN_30", "locus": "GCA009939745_00847" }, { "type": "tRNA", "sequence": "contig_1", "start": 480521, "stop": 480606, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 70.5, "nt": "GCCCAAGTGGCGGAATGGTAGACGCATGGGATTTAAGATCCCACGCTAGCAATAGCGTGCCGGTTCAAGTCCGGCTTTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 480571, 480573 ], "id": "GIHJHMOALN_29", "locus": "GCA009939745_00848" }, { "type": "tRNA", "sequence": "contig_1", "start": 480607, "stop": 480681, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 77.0, "nt": "GACTCACTAGCTCAGCGGTAGAGCATTTGACTTTTAATCAAAGGGTCCCGAGTTCGATCCTCGGGTGAGTCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 480654, 480656 ], "id": "GIHJHMOALN_28", "locus": "GCA009939745_00849" }, { "type": "tRNA", "sequence": "contig_1", "start": 480725, "stop": 480799, "strand": "-", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 70.6, "nt": "TGGGATGTAGCCAAGCGGTAAGGCAATAGACTTTGACTCTATCATGCGATGGTTCGATCCCATCCATCCCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 480765, 480767 ], "id": "GIHJHMOALN_27", "locus": "GCA009939745_00850" }, { "type": "tRNA", "sequence": "contig_1", "start": 480805, "stop": 480891, "strand": "-", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 72.9, "nt": "GGACAGGTAGCGAAGTGGCTAAACGCTTCTGACTGTAGATCAGACACCTTCATGGTTTCGGGAGTTCGAATCTCTCCCTGTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 480883, 480885 ], "id": "GIHJHMOALN_26", "locus": "GCA009939745_00851" }, { "type": "cds", "sequence": "contig_1", "start": 480921, "stop": 481403, "strand": "-", "frame": 2, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "COG:COG0782", "COG:K", "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063", "RefSeq:WP_014325511.1", "SO:0001217", "UniParc:UPI0001DD63D4", "UniRef:UniRef100_A0AAX0SP23", "UniRef:UniRef50_P47524", "UniRef:UniRef90_P78019" ], "nt": "ATGGAACTTAACAAAAATTACCTTACCGAAGAGGGGCTAAAACAATTAGAAGCGGAGCTGGAACACCTCATTCAAGTAAAACGTCCCGCTATTATTAAGTTATTGCAAGAAGCCCGTGACCAGGGTGACTTGAGTGAAAACGCTGATTACGATGCAGCAAAAGCACAGCAGGGTGAAATTGAAACCCGCATTGCTGAAATCCAAGACATTTTAGCCAACGTTAAGTTAATTAATGAGTCGCAAACAAAAAAGGCTAATAAGGTTACCCTGGGTAGTACGGTCGAAATTTACGATTACAGTTCGAAAACACACGAAAAGTACACCATTGTGGGAGCTTTAGAAGCTAATCCCGAAGAACACCGCATCTCCAATGAATCCCCTTTGGCACACGCCATTTATGGTCGTTTAGTGGATGATGAATGTGACGTAGTTGGCATAGAAGTGCCCTACCGGGTCAAAATTGTTAAGATCATTAACGGATAA", "aa": "MELNKNYLTEEGLKQLEAELEHLIQVKRPAIIKLLQEARDQGDLSENADYDAAKAQQGEIETRIAEIQDILANVKLINESQTKKANKVTLGSTVEIYDYSSKTHEKYTIVGALEANPEEHRISNESPLAHAIYGRLVDDECDVVGIEVPYRVKIVKIING", "aa_hexdigest": "3f77c1d22e63dd1d57a5b5856630c969", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63D4", "ncbi_nrp_id": "WP_014325511.1", "uniref100_id": "UniRef100_A0AAX0SP23", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63D4", "RefSeq:WP_014325511.1", "UniRef:UniRef100_A0AAX0SP23" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SP23", "uniref90_id": "UniRef90_P78019" }, "psc": { "uniref90_id": "UniRef90_P78019", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_P47524", "cog_id": "COG0782", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_P47524", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47524" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "GIHJHMOALN_868", "locus": "GCA009939745_00852" }, { "type": "cds", "sequence": "contig_1", "start": 481534, "stop": 481674, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014574968.1", "SO:0001217", "UniParc:UPI0001DD63D5", "UniRef:UniRef100_A0AB38W775", "UniRef:UniRef50_A0AB38W775", "UniRef:UniRef90_A0AB38W775" ], "nt": "ATGACAACGAAAGACAATTCTGGCATTAAAAGTAAAAGACGGATTCGTTTTTTAGCTTTAGGAATTATTATTTTGATTCTTGTAATTGCTGTAATCACCATTTTATTTGTCACGGGTGTAATTACCCGCGCTAACCAATAA", "aa": "MTTKDNSGIKSKRRIRFLALGIIILILVIAVITILFVTGVITRANQ", "aa_hexdigest": "86e8640e47c62ed2fa104c8b81b0bc6b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63D5", "ncbi_nrp_id": "WP_014574968.1", "uniref100_id": "UniRef100_A0AB38W775", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63D5", "RefSeq:WP_014574968.1", "UniRef:UniRef100_A0AB38W775" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W775", "uniref90_id": "UniRef90_A0AB38W775" }, "psc": { "uniref90_id": "UniRef90_A0AB38W775", "uniref50_id": "UniRef50_A0AB38W775" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W775" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5082.190099999999, "isoelectric_point": 11.999967765808105 }, "id": "GIHJHMOALN_869", "locus": "GCA009939745_00853" }, { "type": "cds", "sequence": "contig_1", "start": 481910, "stop": 482134, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGCAAGTTCAACTACCGACCCTTTCCTTTTACAGTGAGTACCAAAAGGAAAAGCGTCATGTGGAGGGCTTTAATCCTGAGCTTTTTTTAATAGAGCAAATAGGTACTAAAAAACTCCATGATCCGCTAGTACTACGTCCTACTAGTGAAATTGCTTTTTGTAACTTATGGAAAAAACAGAGCTTTTCTTACCAAAACCTTCCCGTTATCTATAACCAGTGA", "aa": "MEQVQLPTLSFYSEYQKEKRHVEGFNPELFLIEQIGTKKLHDPLVLRPTSEIAFCNLWKKQSFSYQNLPVIYNQ", "aa_hexdigest": "3447f991c38ca5aaebaa72b61424f513", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8832.037399999997, "isoelectric_point": 6.714697074890137 }, "id": "GIHJHMOALN_870", "locus": "GCA009939745_00854" }, { "type": "cds", "sequence": "contig_1", "start": 482375, "stop": 482572, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCGATGGGCAGGCATTGCAGTGTGCCACGAGCCATGATTTAGGGCAAACCTTTACCAAACCGTTTGGGCTAACCTTTCAAAACCAAGCCAACCAACAGGCCATTCCCTACAGCTTTAGTTGTGGAATTTCTACAAGAATCCTCGGTGCTTTACTGTTAACCCACAGTAATGACTTTGGACTGATACTACCATGA", "aa": "MPDGQALQCATSHDLGQTFTKPFGLTFQNQANQQAIPYSFSCGISTRILGALLLTHSNDFGLILP", "aa_hexdigest": "ee295cf368ce324433738c37e3c7d644", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6996.867099999999, "isoelectric_point": 5.944358253479003 }, "id": "GIHJHMOALN_871", "locus": "GCA009939745_00855" }, { "type": "cds", "sequence": "contig_1", "start": 482651, "stop": 483136, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09180.16" ], "nt": "GTGCAAACGTTGTTGGAACAATTAGCAATCCGTTTTCAATTTATTAAGGTCGAAAACCAGCTCGGTAAACAGTTAGGACAGGGTGAAGTCAACGGCATTCCCTTTCAACTAATAGTTGACAATCCCCAAACAGTCAACATCTTTAACCGGTTAACCAGGGTCAAAACAGCTTACAGTTTCGAACAGTTGGCAAGCCGATTTGTGGAGCTCGTGCAACAGTACCATCAAGCAATGTATGACAAAGCCAAAGCAGTGGTACAGCAAAAGGTGGTGCAAGCCACTACTTTAAAGCAAATTGAACAAGCCTTTAATGACAAAAAGGCTGTTTTGTGTGCGGTTCGTTTAACCGATACATTAGAACAGCAATTAAAGGAACGGTACCAGGTCACGGTGCGCTGTTGCTTAGAGCAGCTTCAAAAGCCCCAAATTTGTCCGTTTAGCGGTGAGTCAGCACAGGACTATGTCCTGATTGCGCGTGCCTATTAA", "aa": "MQTLLEQLAIRFQFIKVENQLGKQLGQGEVNGIPFQLIVDNPQTVNIFNRLTRVKTAYSFEQLASRFVELVQQYHQAMYDKAKAVVQQKVVQATTLKQIEQAFNDKKAVLCAVRLTDTLEQQLKERYQVTVRCCLEQLQKPQICPFSGESAQDYVLIARAY", "aa_hexdigest": "46cf1f9a1daf19c4c12de05acbaa0777", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09180.16", "name": "ProRS-C_1", "length": 70, "aa_cov": 0.40372670807453415, "hmm_cov": 1.0, "evalue": 3.165648316778672e-11, "score": 38.782405853271484, "start": 97, "stop": 161 } ], "seq_stats": { "molecular_weight": 18543.232399999994, "isoelectric_point": 8.897168922424317 }, "id": "GIHJHMOALN_872", "locus": "GCA009939745_00856" }, { "type": "cds", "sequence": "contig_1", "start": 483281, "stop": 483649, "strand": "+", "frame": 2, "gene": null, "product": "putative MG284-like protein", "db_xrefs": [ "RefSeq:WP_010874759.1", "SO:0001217", "UniParc:UPI0000139587", "UniRef:UniRef100_P75381", "UniRef:UniRef50_P47526", "UniRef:UniRef90_P75381" ], "nt": "ATGAGTTTTAAAGTTAAAAACCAGAGTAAGCACCTATACTCTTTAATGACGAAATTTAAACGTTCGCAACTGATCTTGAAGCACCAAAGCAATAACTTTGCCTCAGAGTTGTGGAACGAAGAGGACATTATCCGCTCCAAGCAGTTTATCGAACTTATAGAAGACACGCTGCTCCACCTCAAGAAGGATACGGTCGACTTTATTTACGACATCTTCATTTATGGGAAGAAGCCGTGTGATATTAGTTACTCCAATTCGACCTATTACAAAAAGTTAAACAAGGCTGCAAATAGTTTTTTTGACCATTTTGTCTGGGAGGCTCCAATTTATAAGACAAAGGAGCTTAAAAATGACAATTCTCACAGTTAG", "aa": "MSFKVKNQSKHLYSLMTKFKRSQLILKHQSNNFASELWNEEDIIRSKQFIELIEDTLLHLKKDTVDFIYDIFIYGKKPCDISYSNSTYYKKLNKAANSFFDHFVWEAPIYKTKELKNDNSHS", "aa_hexdigest": "3bb59ed69b6a3d1cb6f566a83d959a59", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139587", "ncbi_nrp_id": "WP_010874759.1", "uniref100_id": "UniRef100_P75381", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139587", "RefSeq:WP_010874759.1", "UniRef:UniRef100_P75381" ] }, "ips": { "uniref100_id": "UniRef100_P75381", "uniref90_id": "UniRef90_P75381" }, "psc": { "uniref90_id": "UniRef90_P75381", "product": "Uncharacterized protein MG284 homolog", "uniref50_id": "UniRef50_P47526" }, "pscc": { "uniref50_id": "UniRef50_P47526", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47526" ], "product": "Uncharacterized protein MG284" }, "genes": [], "id": "GIHJHMOALN_873", "locus": "GCA009939745_00857" }, { "type": "cds", "sequence": "contig_1", "start": 483630, "stop": 483860, "strand": "+", "frame": 3, "gene": null, "product": "DUF5443 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HMP7", "UniRef:UniRef90_A0AB33HMP7" ], "nt": "ATGACAATTCTCACAGTTAGACAAATTCAAAATAATTACTATACGGAATACTACATGGGTCCGGTTAGAAACTTTGCTGACAAAAAGGAAGTTTACTTTGATGCTAAGGTAAACAATATTGAAAGCAAAGTCAACAAGGATAAAGCCTTGTTACACATAACCCTGGCGTTAAAGTACGACAGTAACTTTCCCAGTAATATGTTCCAAGCTCAGTTTAAACTGGGTAATTGA", "aa": "MTILTVRQIQNNYYTEYYMGPVRNFADKKEVYFDAKVNNIESKVNKDKALLHITLALKYDSNFPSNMFQAQFKLGN", "aa_hexdigest": "3db421df654ed2b4b39175648bdcad95", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 483630, "stop": 484013, "observations": { "mutation": [ 483858 ], "stop": [ 483858 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HMP7", "query_cov": 1.0, "subject_cov": 0.5984251968503937, "identity": 1.0, "score": 154.0, "evalue": 6.73e-47, "gene_start": 1, "gene_end": 76, "reference_start": 1, "reference_end": 76, "reference_sequence": "MTILTVRQIQNNYYTEYYMGPVRNFADKKEVYFDAKVNNIESKVNKDKALLHITLALKYDSNFPSNMFQAQFKLGNWSSEKIPLQKAPDKNPIWKIRFTIFMPLWKCHELPLLNGKLTALVSIFVKN" }, "paralog": false, "identity": 0.9921259842519685, "score": 258.0, "evalue": 1.51e-90, "description": "Internal stop codon at 483858. Nonsense mutation around 483858.", "psc": { "uniref90_id": "UniRef90_A0AB33HMP7", "product": "Family of unknown function (DUF5443)", "uniref50_id": "UniRef50_A0AB33HMP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HMP7", "UniRef:UniRef50_A0AB33HMP7" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HMP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HMP7" ] } }, "id": "GIHJHMOALN_874", "locus": "GCA009939745_00858" }, { "type": "cds", "sequence": "contig_1", "start": 483947, "stop": 484207, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCACGAACTGCCCTTATTAAACGGGAAATTAACCGCATTAGTGAGTATATTCGTGAAGAATTAACAATTGCTTTTCGCTTAAATGACAGTAGTGATCGCTACCATTGGAGTAACTATAACCTGTTCGATACAGTGGCAGCTGATATGTACCAAACGGTCATTAAAGAAACCGTTACCTTTGGTAACATGATTAGACTAAACCAACTCGAGGGCGAGAAGGAAGGTAACATCCAACAAAGTGAACTTAAATATGGTTGA", "aa": "MPRTALIKREINRISEYIREELTIAFRLNDSSDRYHWSNYNLFDTVAADMYQTVIKETVTFGNMIRLNQLEGEKEGNIQQSELKYG", "aa_hexdigest": "9a95947fe0086d9d3decd8c19b46e65b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10178.338500000002, "isoelectric_point": 5.350277137756348 }, "id": "GIHJHMOALN_875", "locus": "GCA009939745_00859" }, { "type": "cds", "sequence": "contig_1", "start": 484211, "stop": 484669, "strand": "+", "frame": 2, "gene": null, "product": "DUF5443 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W764", "UniRef:UniRef90_A0AB38W764" ], "nt": "ATGTTGGACTACCGCAATATGGGGCCGCTGGAAGAGGTTAATAATTTAATTAATATTAAGTTTAACCAACCAGTAAAAGTGGTAAGTGTTGGTGTGACCGTAAGCTATACAGCGCCTGATGGTAAACGCCAAGAGCTCAAAGATCAAGCTGAATACCAGCACACCCTCAACCCCAATGAAGAGTTCAAACTGAACTTTCCCAGACGGCTAAGCTTTAACAGGGTAACTAAAAAGTTGGACTTTGACCCCAATGGTAGTGCCGTGTTCCTACCACAGGGTGGCTTTGGTAGCTATGAGATCAAACTGGAGGCTAATGTCGGCAACCAGTTCTACACAATTGTGGCTACTAACAGCTTTGAGTACGCCCATCCGTTTGACGATCCCAAGACCAATGACTTCTTTCTGGTGCAGTACGCTCCAGTCTACAGTCTGTTTAACTTTAGCGATTTAATTCAGTAA", "aa": "MLDYRNMGPLEEVNNLINIKFNQPVKVVSVGVTVSYTAPDGKRQELKDQAEYQHTLNPNEEFKLNFPRRLSFNRVTKKLDFDPNGSAVFLPQGGFGSYEIKLEANVGNQFYTIVATNSFEYAHPFDDPKTNDFFLVQYAPVYSLFNFSDLIQ", "aa_hexdigest": "e0cfce36bda916afae4f31fcb9de000c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 483947, "stop": 484669, "observations": { "mutation": [ 484205 ], "stop": [ 484205 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W764", "query_cov": 1.0, "subject_cov": 0.6333333333333333, "identity": 1.0, "score": 311.0, "evalue": 3.25e-106, "gene_start": 1, "gene_end": 152, "reference_start": 89, "reference_end": 240, "reference_sequence": "MPRTALIKREINRISEYIREELTIAFRLNDSSDRYHWSNYNLFDTVAADMYQTVIKETVTFGNMIRLNQLEGEKEGNIQQSELKYGWTMLDYRNMGPLEEVNNLINIKFNQPVKVVSVGVTVSYTAPDGKRQELKDQAEYQHTLNPNEEFKLNFPRRLSFNRVTKKLDFDPNGSAVFLPQGGFGSYEIKLEANVGNQFYTIVATNSFEYAHPFDDPKTNDFFLVQYAPVYSLFNFSDLIQ" }, "paralog": false, "identity": 0.9916666666666667, "score": 482.0, "evalue": 5.54e-176, "description": "Internal stop codon at 484205. Nonsense mutation around 484205.", "psc": { "uniref90_id": "UniRef90_A0AB38W764", "product": "Family of unknown function (DUF5443)", "uniref50_id": "UniRef50_A0AB38W764", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W764", "UniRef:UniRef50_A0AB38W764" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W764", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W764" ] } }, "id": "GIHJHMOALN_876", "locus": "GCA009939745_00860" }, { "type": "cds", "sequence": "contig_1", "start": 484676, "stop": 485188, "strand": "+", "frame": 2, "gene": null, "product": "putative MG286-like protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47528", "UniRef:UniRef90_P75379" ], "nt": "ATGATCTTCTCAATTAGTAAACGCAAGTTAATTTGTGGCTTTTCCCTAGTAGCCCTTACAATCGCTGGGATTGTTGGTGGGGTTTACTTAGTCACCAAAAACAATCAACAAACCACACCACAAACAAATCACTTTAATGTAGCTGAACCAGTCAACAAGGTCCCTAATTGGCGCAAACTAGGTCCAGAAACACAACGCGAACTGCGCGATCGTCTGTATCCCTTAGATGACACAGGTTACTTTATTTACAAGTACGGTGCCATTAGTCGCTACCTCCAAAGCCAAAAGGAACTGGATGAACTAGTCGATTACCGCACCGTGTTACCCTCCACCCAAAAGCACTTTAAGTATGATTCCTTTAACCAAAGCGTGTTGGAAAGTAAGCTGCGTAAGTGGTTAATGAAAGCCATTAAACAACACCCGTACTTTCAGCACTTTGAGTTTGATCCGGTGCTTAAAGCACAGTACAATATCAACATTCCTGCTCAAAAAATTACGGTGAATGCAGTTTGA", "aa": "MIFSISKRKLICGFSLVALTIAGIVGGVYLVTKNNQQTTPQTNHFNVAEPVNKVPNWRKLGPETQRELRDRLYPLDDTGYFIYKYGAISRYLQSQKELDELVDYRTVLPSTQKHFKYDSFNQSVLESKLRKWLMKAIKQHPYFQHFEFDPVLKAQYNINIPAQKITVNAV", "aa_hexdigest": "a2e5132a8632cee9d8dbfd34225ebd28", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75379", "query_cov": 1.0, "subject_cov": 0.8629441624365483, "identity": 0.988, "score": 340.0, "evalue": 3.99e-118, "valid": true, "product": "Uncharacterized protein MG286 homolog", "uniref50_id": "UniRef50_P47528", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47528", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47528" ], "product": "Uncharacterized protein MG286" }, "genes": [], "id": "GIHJHMOALN_877", "locus": "GCA009939745_00861" }, { "type": "tRNA", "sequence": "contig_1", "start": 485549, "stop": 485623, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 70.7, "nt": "AGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCA", "db_xrefs": [], "id": "GIHJHMOALN_12", "locus": "GCA009939745_00862" }, { "type": "cds", "sequence": "contig_1", "start": 485813, "stop": 485956, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SQH8", "UniRef:UniRef90_A0AAX0SQH8" ], "nt": "GTGGATGATCAAACCGGTGTGCTCGACATTAAGCAGTCTGTACCACCCAAACTAAAGGTTAACAAACAAGGCTTTGAACCTGTTTTCATCACCTTTACTAACACTAACAACAACGAGCAAGTTACCGTGGTTTTAAACAACTGA", "aa": "MDDQTGVLDIKQSVPPKLKVNKQGFEPVFITFTNTNNNEQVTVVLNN", "aa_hexdigest": "4d88cce42c741a7f190bb9682c1e8a27", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 485663, "stop": 485956, "observations": { "mutation": [ 485776, 485806, 485815 ], "stop": [ 485776, 485806, 485815 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAX0SQH8", "query_cov": 1.0, "subject_cov": 0.4845360824742268, "identity": 1.0, "score": 95.5, "evalue": 1.17e-24, "gene_start": 1, "gene_end": 47, "reference_start": 1, "reference_end": 47, "reference_sequence": "MDDQTGVLDIKQSVPPKLKVNKQGFEPVFITFTNTNNNEQVTVVLNNWSQWETHKTDFAPWLKCKHWNIDVKQIRRVKTTFRDQENANFFELIESKQ" }, "paralog": false, "identity": 0.9587628865979382, "score": 189.0, "evalue": 6.25e-64, "description": "Internal stop codon at 485776, 485806, 485815. Nonsense mutation around 485776, 485806, 485815.", "psc": { "uniref90_id": "UniRef90_A0AAX0SQH8", "uniref50_id": "UniRef50_A0AAX0SQH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAX0SQH8", "UniRef:UniRef50_A0AAX0SQH8" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SQH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SQH8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5275.897100000002, "isoelectric_point": 4.8610639572143555 }, "id": "GIHJHMOALN_878", "locus": "GCA009939745_00863" }, { "type": "cds", "sequence": "contig_1", "start": 486044, "stop": 486214, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCTTACAGTTAGCTAAAGTGAGTGGGATTGATATTAATGAAAACAAACTGTTTAACACTGATTTTTTAAAGAAGTCGTTTTACTTTGCCGATAAATTTTCCCACCTTTCAGTGAACGGTAATGTGGAATACATGCGCAATATCTTAACAACTGTTGGGTTTGATTGA", "aa": "MFLQLAKVSGIDINENKLFNTDFLKKSFYFADKFSHLSVNGNVEYMRNILTTVGFD", "aa_hexdigest": "1c83aba5ab5176eff1197662a976d41e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6482.352200000001, "isoelectric_point": 6.53480167388916 }, "id": "GIHJHMOALN_879", "locus": "GCA009939745_00864" }, { "type": "cds", "sequence": "contig_1", "start": 486296, "stop": 486679, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13472.12" ], "nt": "TTGTTTATTGGTGATTCGATGACTCATGGTGTTGGTACTGACGGATACAGTAGTGCCCCACAACTGTTGGAACAAAGCTTGAAAAATGACTTTGGTCGTTACCGTGATGTAGTGATCAACAGTGCCATTAATGGTAGTAACACCTCCTTAGAGTTGTACATGCAAAATCATCGCTTTAAGCAGTACAACAACATTGATGTTTATGTACTTAATTTGGGTACTAATGACATTAACCAGTTAGCGCAAGGGGTTTACACAGTCGAGCAATACAAACATAATCTTACCCAAATACTGGATCTGTTACATACGCAAAGTCCTCAAGCCCATGTTGTTTTAGCCAACATTGCTCCATCTAGCTTACTGCGAAGCTCTGAAAAGAACTGA", "aa": "MFIGDSMTHGVGTDGYSSAPQLLEQSLKNDFGRYRDVVINSAINGSNTSLELYMQNHRFKQYNNIDVYVLNLGTNDINQLAQGVYTVEQYKHNLTQILDLLHTQSPQAHVVLANIAPSSLLRSSEKN", "aa_hexdigest": "6cd9378016ec3d861fc4cff1a90e9399", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13472.12", "name": "Lipase_GDSL_2", "length": 118, "aa_cov": 0.9133858267716536, "hmm_cov": 0.9491525423728814, "evalue": 9.92436067205337e-20, "score": 67.23892211914062, "start": 2, "stop": 117 } ], "seq_stats": { "molecular_weight": 14213.683699999985, "isoelectric_point": 5.870183372497558 }, "id": "GIHJHMOALN_880", "locus": "GCA009939745_00865" }, { "type": "cds", "sequence": "contig_1", "start": 487034, "stop": 487348, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTGAAGACAAAGTTAGCTTAAGTTTGCAAGGAGAATTTGCGATTGGTAAACAGTTAGCACAAGTGTTAAAAGCTACTGGCGCTGAACCATTGCCAGTGGAAGTTACAGAACCATCACTAGAAGCTATACCAATTAATGAAGAAACCCAGTTTGATGTAAAAAATTACTACAGCTTTGGCCATTACCATGACACCAATGTCATTAATGACAGGATTGATTTTGATGATCCCAACTACAACTTAGAAACGGACTTTTTCAATAATGCTAAAGCTAATTTGGTAACTACTTCCAATCCCTTTACTGTTACTTGA", "aa": "MSEDKVSLSLQGEFAIGKQLAQVLKATGAEPLPVEVTEPSLEAIPINEETQFDVKNYYSFGHYHDTNVINDRIDFDDPNYNLETDFFNNAKANLVTTSNPFTVT", "aa_hexdigest": "c39648a9d1d8352e338100dbdf228006", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11644.65189999998, "isoelectric_point": 4.223273658752441 }, "id": "GIHJHMOALN_881", "locus": "GCA009939745_00866" }, { "type": "cds", "sequence": "contig_1", "start": 487517, "stop": 487777, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGCTAGTGCTCGGTTTGAAGGTAAGATAGCAAACTTTGGTTTTGCCGGGCAGACTATGAAAGATATTAATGAGAGCTTTCAAGAACGGGTACTTTCAATTTACAAACCACGTACGCTAGTTTACCTCACTTCCCAAGCGGATGCAGAGCGGGTTGATTATTACGAGGAGTTTGTCAATAATCTAACCGCACTCATTAAAAAGCAAGTTGGTGAAAAGGCTGAAAAGGACAACTTTTTCATTTTGCAATTGCACTGA", "aa": "MFASARFEGKIANFGFAGQTMKDINESFQERVLSIYKPRTLVYLTSQADAERVDYYEEFVNNLTALIKKQVGEKAEKDNFFILQLH", "aa_hexdigest": "10e8c22a9cf686b7f2745657e6e1dd78", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9974.213899999993, "isoelectric_point": 5.775148582458496 }, "id": "GIHJHMOALN_882", "locus": "GCA009939745_00867" }, { "type": "cds", "sequence": "contig_1", "start": 487973, "stop": 488209, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCACTCAGAGTGATGTTTCACCACTCTTTGTGAAAAATATCAACATCCAACTACCGAGCTCCACCCATCAACAAAAGATTGAACCCTTAAATTTACCAACTAACATTAGCAAAACAGAAATAGCGAGTTGGGGTAGTGAATTCCAAAGCAAGGTATTGGACTTTTATGCTAGGGATGGTGAAGAACATGACAACAAAATTAGGTACCAACTCCCATCCTTAAGCTTTGCTTGA", "aa": "MGTQSDVSPLFVKNINIQLPSSTHQQKIEPLNLPTNISKTEIASWGSEFQSKVLDFYARDGEEHDNKIRYQLPSLSFA", "aa_hexdigest": "c33f8049d6badddb7a1fe65f6e5d6cd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8836.776099999992, "isoelectric_point": 5.513689231872558 }, "id": "GIHJHMOALN_883", "locus": "GCA009939745_00868" }, { "type": "cds", "sequence": "contig_1", "start": 488530, "stop": 489162, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTAAGAAGGGTTTTTTCGCGTTATCAAGTTGCACATTAGGTCTAGGTCTTATTTTAACTGCATGTGGTGCTAGGGGTAAATTTGACCAAGTTGACGATGGTGTTATTAAGCTAGCTACTTCAATCCAAAATAAGGACAGTATCGCTGCTTTAAACACCATTTACAAGAAGTATAAGGAACGCCATCCAGGGAGTTATCCAGTTCAAAATTTTCAGGTTCCCGGTGGTTACAGTGGTTTACGGAACGACATTAGAACGCGTTTATCAGCTAAAGATGGCAACAACTTTTACAACATAGTACTCAACTATCCTGATGTTGTCTCCTCATTGGCTACAAGTGACATGCAACTAATATTGGATAACGTTGACACCAAGCTCCTTTCGAAAAATTTCTTGAGCTTTAACGAGCGAATTGGTGGTGTACGACAAAAGGGGATTTATGCAATTCCCATTTCCCTTTCCACTGAATTAATGGTGCTCAACGGACCGGTATTGCACTATATTTTGAACAGTGCTAAGAAGAAGGAAAACGGTGCTCAAATGATTAAAAGATCAGGTAGTTTTTCCACTGTTCAAAAAGGTACCATGGCAATCGACATGAATGATCAAAAAACCAAAGATCTTTGA", "aa": "MVIKKGFFALSSCTLGLGLILTACGARGKFDQVDDGVIKLATSIQNKDSIAALNTIYKKYKERHPGSYPVQNFQVPGGYSGLRNDIRTRLSAKDGNNFYNIVLNYPDVVSSLATSDMQLILDNVDTKLLSKNFLSFNERIGGVRQKGIYAIPISLSTELMVLNGPVLHYILNSAKKKENGAQMIKRSGSFSTVQKGTMAIDMNDQKTKDL", "aa_hexdigest": "fcd1555589bc2af8d9d9776fd99df763", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23061.360500000003, "isoelectric_point": 9.57634334564209 }, "id": "GIHJHMOALN_884", "locus": "GCA009939745_00869" }, { "type": "cds", "sequence": "contig_1", "start": 489694, "stop": 490476, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTACATTAATAAAGGTGGTGCTTATTCCAGTAACTTCTTAAAGTTTCACCAGTTAGCTTTTAGTGTGGGTTCCAGTTCTGGTTACAATTTTGCTTTTGCTGGTGAAAGCGCTAAACGCTTGGAATTTGGCCAGAAAGCAATCGAGTACCCACGTGACACTTACGAAATTAAGGCACCTACAAATAGCCAAAATGGTAATGGTACCCTTCTAGGTAGTTTTACTAAAAGTAAAAGTAATGGCAAAGAACAAAGTGGGCAAGATGAGGATAACCAGACCAGTGAAACGATTGAGCTTTACAAAAGCAGCGTTCCTAGTGGTAAAGAGGCGGGTAAAAATGCGCTTGCTATTACCAATCAACAACTAATTTCGGCTTTAGAAAATGCTGCAAAAGATAATAAAACCTCCCAACCACAAGCACGATCACTTACAGCTTCTGATCAAGTTCAAATTACACAAAGTTCAGATAAGGTTATTGGTTACATCACCACATCTAATTTGGACATTGACAACAACAATACCTTTGATGTTGGTAAGTTAAACGGTGATAAGTCAACCAGCAAAATCATTGTTAACGCTACTTTAAAAACCTTAAACAAGATCAACACCTTGCAAAGCGAAGAGGGCATTATTCTGCCCCATCCACAAAAATATAAGAGTACTGATCCACAAGCTGTCGCTACCGTTCAGGGTCCAAGTATTATTGGTGTTCATGCTAACGCTAAGGAAAACGCTGAAACCCAAAAATTTATCAACTGGTTTATTAACCAAAAAGAAACTTGA", "aa": "MYINKGGAYSSNFLKFHQLAFSVGSSSGYNFAFAGESAKRLEFGQKAIEYPRDTYEIKAPTNSQNGNGTLLGSFTKSKSNGKEQSGQDEDNQTSETIELYKSSVPSGKEAGKNALAITNQQLISALENAAKDNKTSQPQARSLTASDQVQITQSSDKVIGYITTSNLDIDNNNTFDVGKLNGDKSTSKIIVNATLKTLNKINTLQSEEGIILPHPQKYKSTDPQAVATVQGPSIIGVHANAKENAETQKFINWFINQKET", "aa_hexdigest": "7ae7bafaccd93631191795a44a500021", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28211.81710000002, "isoelectric_point": 7.787539100646972 }, "id": "GIHJHMOALN_885", "locus": "GCA009939745_00870" }, { "type": "cds", "sequence": "contig_1", "start": 490519, "stop": 490812, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGCAGCACAATACTTTGCTAAGAGCTCTGGTTATGTTTTACCTTACAGTGAAACGTTTACTAAACAAAGTGAAGACGAACACAGCAAAACTAAAGACGCTTATAAGATCTTGAAAGATGTAAACGATGGTAAGTTAGTTGGTTACAGTGATCCAAGTGATTTCCGTTCTGGTAAATTCCGTGACACAATTGTCGCTGCTTTTAGTGGTGCTGTAAGCTCTAAAGCTGACTTTAATAAGTTCTTTAAAGATTTTGAACAACAATTAGGTCAAGAATACAGAAGGGGTTAA", "aa": "MTAAQYFAKSSGYVLPYSETFTKQSEDEHSKTKDAYKILKDVNDGKLVGYSDPSDFRSGKFRDTIVAAFSGAVSSKADFNKFFKDFEQQLGQEYRRG", "aa_hexdigest": "0494f99cd7036ad6b4430385e379a99f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10974.982999999993, "isoelectric_point": 7.886820411682128 }, "id": "GIHJHMOALN_886", "locus": "GCA009939745_00871" }, { "type": "cds", "sequence": "contig_1", "start": 490786, "stop": 491202, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ], "nt": "GTGGTTTTCGTCACTTTGTCCGTTCTTATCATTCCTTCACCCCGCGCCGGTTCAGAGCAGGAGGTTGGCTTGGTCGAGGGTGGGTCATTTGGAGCCGCCTGTAGTCGTCGTCTGACTGCCACCAAGCGTGGCTTGGACGTTGGCTTTGAGGCCAACGCCGACATTCGAGAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCCGGTTTTAGGCGCGGTTATATCATCCACGCCCCGGTGGTTCTTCCCATACCTGTACCCATTCCCAATCCAGGGCACGTAATCCAACACCTGGTCAAGGAGTGCCCCCGCGAGGTTGAACCCGGTGGAGAACCGGGAAAGGGTGGGGGAGAAGTGGGATTAACCCCTTCTGTATTCTTGACCTAA", "aa": "MVFVTLSVLIIPSPRAGSEQEVGLVEGGSFGAACSRRLTATKRGLDVGFEANADIRERREKGTRTTCVRTSSAGRPRAGFRRGYIIHAPVVLPIPVPIPNPGHVIQHLVKECPREVEPGGEPGKGGGEVGLTPSVFLT", "aa_hexdigest": "dba157ce30a27db0a75f7266c2a55337", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027E159D2", "query_cov": 0.9710144927536232, "subject_cov": 0.950354609929078, "identity": 0.657, "score": 154.0, "evalue": 6.25e-46, "valid": false, "uniref50_id": "UniRef50_A0AB38W7Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14606.652599999985, "isoelectric_point": 9.635009574890137 }, "id": "GIHJHMOALN_887", "locus": "GCA009939745_00872" }, { "type": "cds", "sequence": "contig_1", "start": 491992, "stop": 492513, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03257.18" ], "nt": "GTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAACTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTGGTACCACCACCGCAACAGGGGCTGCCTTACCGGAGCACCCGAATGCTTTGGCGTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCATTTGATTAAACCTAAGAAAGTTACCCAATCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGCGCCAGAATGCTCCAATCATTTGGTACAGAAAATTCCACCCAAGCCCAGCCCCAGTCGCTCAAAACAACGACACCGGTGTTTGGGGCCATGAGTGGTAACCTCGGCAGTGTGCTTAGTGGTGGGGGTACCACGCAGGGCTCAAGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACTATTAATAGGAACTTTTCTTATTAA", "aa": "MWDFKGVWADQTHSAVSESQAATSGTTTATGAALPEHPNALAFQVSVVEASAYKPNTSSGQTQSTNSSPYLHLIKPKKVTQSDKLDDDLKNLLDPNEVRARMLQSFGTENSTQAQPQSLKTTTPVFGAMSGNLGSVLSGGGTTQGSSSGQSGVDLSPVERVSGSLTINRNFSY", "aa_hexdigest": "4e60f2541ab001f58a0fe9dc731f622d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03257.18", "name": "Adhesin_P1_C", "length": 116, "aa_cov": 0.6647398843930635, "hmm_cov": 0.9913793103448276, "evalue": 2.6775843710949633e-55, "score": 181.34796142578125, "start": 49, "stop": 163 } ], "seq_stats": { "molecular_weight": 18139.678299999978, "isoelectric_point": 5.85585994720459 }, "id": "GIHJHMOALN_888", "locus": "GCA009939745_00873" }, { "type": "cds", "sequence": "contig_1", "start": 492599, "stop": 492850, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532788.1", "SO:0001217", "UniParc:UPI0002D77CA5", "UniRef:UniRef100_A0AB38W9C2", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0A0H3DNZ5" ], "nt": "ATGAAATTAGTTAAATTAGCGAGTTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTATTGGCGCTTTTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGTGTTACTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAA", "aa": "MKLVKLASFHRFLKQDQLQLVPLFSPNFLQVLLALFNKPVNLFSENTTMKTNKKYAFAVLLMQAQAKLPNSFKFFLSKKGGMA", "aa_hexdigest": "a0d78bba7fd026ec85ef7e81cbeb0658", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002D77CA5", "ncbi_nrp_id": "WP_017532788.1", "uniref100_id": "UniRef100_A0AB38W9C2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D77CA5", "RefSeq:WP_017532788.1", "UniRef:UniRef100_A0AB38W9C2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W9C2", "uniref90_id": "UniRef90_A0A0H3DNZ5" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DNZ5", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9510.348899999994, "isoelectric_point": 10.471680259704591 }, "id": "GIHJHMOALN_889", "locus": "GCA009939745_00874" }, { "type": "cds", "sequence": "contig_1", "start": 493018, "stop": 493296, "strand": "+", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_410", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572", "UniRef:UniRef90_P75572" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTATAAAGATTGAAACCACTTTGACCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGTTTGGACAAAATTGATCCACCCAAATAG", "aa": "MNTEYVTRKEFNEYKDSNDQRLIKIETTLTAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPPK", "aa_hexdigest": "f539444a590abb9fb0a5e5098d17bb04", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 492850, "stop": 493296, "observations": { "mutation": [ 492931 ], "stop": [ 492931 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75572", "query_cov": 1.0, "subject_cov": 0.6216216216216216, "identity": 0.935, "score": 105.0, "evalue": 4.96e-27, "gene_start": 1, "gene_end": 92, "reference_start": 57, "reference_end": 148, "reference_sequence": "MKEKTPFKNEKEFHEMVKKTKKGTFSGWYIIDKDNNSVEFSGKFNRQFKLNKPVITVNTEYVTRKELNEYKDSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQIRELQVEQKAQGEQIKAQGETLKLILQTLQKMSDRLDKIDPSK" }, "paralog": false, "identity": 0.8918918918918919, "score": 201.0, "evalue": 2.92e-67, "description": "Internal stop codon at 492931. Nonsense mutation around 492931.", "psc": { "uniref90_id": "UniRef90_P75572", "product": "UPF0134 protein MPN_410", "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75572", "UniRef:UniRef50_P75572" ] }, "pscc": { "uniref50_id": "UniRef50_P75572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75572" ], "product": "UPF0134 protein MPN_204" } }, "id": "GIHJHMOALN_890", "locus": "GCA009939745_00875" }, { "type": "cds", "sequence": "contig_1", "start": 493567, "stop": 493860, "strand": "-", "frame": 1, "gene": null, "product": "lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75101", "UniRef:UniRef90_UPI0013300FD7" ], "nt": "ATGGAGCGGAAAGTTGAGTATTTCAATATGGAATATAAAAGCTTTAGTGACTTTTCAGTTAATGCTAGGTTAAGCTATATCTTTAATTGGTATGGGAGTTATTTTGGAGAAAAAAGTTTCACTGCCAATAATGGCGGTAAGCATAATTTCGATCTTTTCCTTAGTATTAAATCTCATTCAAAAAAACAATTTACAGAAAAAAGTTTCATTGTTAAAGATGAAAATTTTCAAGATCAAGATAAAAGTCAACAAATAATTACAGAATGGATTCAGCTTGATCTTTCACTGATTTGA", "aa": "MERKVEYFNMEYKSFSDFSVNARLSYIFNWYGSYFGEKSFTANNGGKHNFDLFLSIKSHSKKQFTEKSFIVKDENFQDQDKSQQIITEWIQLDLSLI", "aa_hexdigest": "3d7eea4762cde666b25b7dc2b6c60885", "start_type": "ATG", "rbs_motif": "AAAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 493615, "stop": 494157, "observations": { "mutation": [ 493950 ], "stop": [ 493950 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013300FD7", "query_cov": 0.8350515463917526, "subject_cov": 0.41968911917098445, "identity": 1.0, "score": 162.0, "evalue": 3.91e-49, "gene_start": 1, "gene_end": 81, "reference_start": 113, "reference_end": 193, "reference_sequence": "MRKKKFLSRFSFSSLFLLCGTLLSACTGIQADLRNLIKETTGKDIDLSKAIKTKEGKKNIITSLKKSYEVNPKDTTKLLLDAWKQSFEEGKLGIAELAFDQATNPTKNSDFKMERKVEYFNMEYKSFSDFSVNARLSYIFNWYGSYFGEKSFTANNGGKHNFDLFLSIKSHSKKQFTEKSFIVKDENFQDQDK" }, "paralog": false, "identity": 0.9666666666666667, "score": 351.0, "evalue": 1.02e-125, "description": "Internal stop codon at 493950. Nonsense mutation around 493950.", "psc": { "uniref90_id": "UniRef90_UPI0013300FD7", "product": "lipoprotein", "uniref50_id": "UniRef50_P75101", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013300FD7", "UniRef:UniRef50_P75101" ] }, "pscc": { "uniref50_id": "UniRef50_P75101", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75101" ], "product": "Uncharacterized protein MPN_012" } }, "id": "GIHJHMOALN_891", "locus": "GCA009939745_00876" }, { "type": "cds", "sequence": "contig_1", "start": 493948, "stop": 494196, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGCGCAAAAAGAAATTTTTGTCAAGGTTTGCTTTTGGTTCTTTATTTTTGTTATGTGGAACAATTTTAAGCGCTTGCACTGGTATCCAAGCTGATCTTAGAAATCTTATTAAAGAAGCCACCGGTAAAGACATTGATTTGTCTAAATCAATTAAAACTAAAGATGGTAAAAAGAACATAATAACTTCTTCAAAGAAGTCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCATGA", "aa": "MRKKKFLSRFAFGSLFLLCGTILSACTGIQADLRNLIKEATGKDIDLSKSIKTKDGKKNIITSSKKSYEVNPKDTTKLLLDA", "aa_hexdigest": "013702273c8f33805e08906a36769aed", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 0.8540000000000001, "score": 136.0, "evalue": 4e-40, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "GIHJHMOALN_892", "locus": "GCA009939745_00877" }, { "type": "cds", "sequence": "contig_1", "start": 494703, "stop": 495155, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321", "UniRef:UniRef50_UPI001375E5B8", "UniRef:UniRef90_Q9EXD5", "UniRef:UniRef90_UPI001375E5B8" ], "nt": "GTGGTGTACTATGAGCAGTTGCAGCGGGGGTTGAGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCAACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGGATGCCCAAAAAGACGACCAGAAGTCCTCGGAATATTTAAGTGGGGAGGCGGGAAGTCAAGCGGGTGGATCCACCCAAGGTACTTCCACCACCACCCAACGTAGGGGTTCGTCAAATGAAAACAAAGTCAAGGTGTTGCAGGTGGCGATGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACCACCTCCAGTTCGGAATCTGGTCAATCAGGTACTCAAGGATCCACCCCAAGTAATCCGGGACCCTGA", "aa": "MVYYEQLQRGLSLPQQRTENGQNTSTTGAMFGLKVKDAQKDDQKSSEYLSGEAGSQAGGSTQGTSTTTQRRGSSNENKVKVLQVAMKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTTSSSESGQSGTQGSTPSNPGP", "aa_hexdigest": "532e40a9f393c2a2b370fb8f0e9f68c0", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001375E5B8", "query_cov": 0.86, "subject_cov": 0.8486842105263158, "identity": 0.9840000000000001, "score": 232.0, "evalue": 2.55e-76, "valid": true, "uniref50_id": "UniRef50_UPI001375E5B8" }, "pscc": { "uniref50_id": "UniRef50_UPI001375E5B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001375E5B8" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 494703, "stop": 495458, "observations": { "mutation": [ 495153, 495315, 495330, 495354 ], "stop": [ 495153, 495315, 495330, 495354 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9EXD5", "query_cov": 0.9, "subject_cov": 0.43973941368078173, "identity": 0.993, "score": 242.0, "evalue": 6.21e-78, "gene_start": 1, "gene_end": 135, "reference_start": 1, "reference_end": 135, "reference_sequence": "MVYYEQLQRGLGLPQQRTENGQNTSTTGAMFGLKVKDAQKDDQKSSEYLSGEAGSQAGGSTQGTSTTTQRRGSSNENKVKVLQVAMKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTTSSSESGQSGTQGSTPSNPGPWTPWLTTEQIHNDPAKFAASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFGQALLVFGICLS" }, "paralog": false, "identity": 0.9043824701195219, "score": 447.0, "evalue": 4.11e-161, "description": "Internal stop codon at 495153, 495315, 495330, 495354. Nonsense mutation around 495153, 495315, 495330, 495354.", "psc": { "uniref90_id": "UniRef90_Q9EXD5", "product": "Uncharacterized protein MPN_412", "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9EXD5", "UniRef:UniRef50_P75321" ] }, "pscc": { "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321" ], "product": "Uncharacterized protein MPN_462" } }, "id": "GIHJHMOALN_893", "locus": "GCA009939745_00878" }, { "type": "cds", "sequence": "contig_1", "start": 495372, "stop": 495626, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAA", "aa": "MLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFGQALLVFGICLS", "aa_hexdigest": "4a15d86ad48e1cca9c8912204bb43c80", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 77, "aa_cov": 0.9166666666666666, "hmm_cov": 0.6623376623376623, "evalue": 2.274810953133893e-17, "score": 58.75332260131836, "start": 4, "stop": 80 } ], "seq_stats": { "molecular_weight": 9349.227799999997, "isoelectric_point": 4.498829078674317 }, "id": "GIHJHMOALN_894", "locus": "GCA009939745_00879" }, { "type": "cds", "sequence": "contig_1", "start": 495771, "stop": 496235, "strand": "+", "frame": 3, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_A0AB38W808" ], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGA", "aa": "MGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPKATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLEQTTQ", "aa_hexdigest": "13df9b633592fd302001eeaf1764e1aa", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 495720, "stop": 496407, "observations": { "insertion": [ 496350 ], "mutation": [ 495762, 496233, 496341 ], "stop": [ 495762, 496233, 496341 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W808", "query_cov": 0.974025974025974, "subject_cov": 0.6329113924050633, "identity": 0.987, "score": 284.0, "evalue": 1.45e-95, "gene_start": 1, "gene_end": 150, "reference_start": 17, "reference_end": 166, "reference_sequence": "MRAAHLHTSYELVDWKVGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESGWDNQRSQKNLTNNPGPKAVTGFKLDKGRAYRKLNESWPVSNPSIRPRLARGKVPQNEKRRRQMLRVMLP" }, "paralog": false, "identity": 0.8859649122807017, "score": 380.0, "evalue": 1.12e-135, "description": "Internal stop codon at 495762, 496233, 496341. Frameshift due to insertion around 496350. Nonsense mutation around 495762, 496233, 496341.", "psc": { "uniref90_id": "UniRef90_A0AB38W808", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W808", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "GIHJHMOALN_895", "locus": "GCA009939745_00880" }, { "type": "cds", "sequence": "contig_1", "start": 496446, "stop": 496673, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTTTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGTGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MSSTGSQMAAVTDSQQSGHNSGLVSLAQRSTTVAVQKSDSSGFQGQGTTDNKFQKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "77a80305a02f969b876e134cf9a2a425", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7832.513400000002, "isoelectric_point": 6.697645378112793 }, "id": "GIHJHMOALN_896", "locus": "GCA009939745_00881" }, { "type": "cds", "sequence": "contig_1", "start": 496932, "stop": 497615, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI002246AD21" ], "nt": "TTGAACTTCGGCACTGAAGTTCAAAAAAGCTTCGTTGAAAACCAGTTGGGGTTTAAAGATGACTCCAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGACCTCACCCAACCCGCCTACCTCATCACTGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCGTTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGATCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGA", "aa": "MNFGTEVQKSFVENQLGFKDDSNSDSKNSNLKAQDLTQPAYLITGLDVVQDHLVFAAFKAGAVGYDMTTDSNASTKDQALAWSTTAGLDSAGGYNNLVENTAGLNGPINDLFTLLDTFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLDEARLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVK", "aa_hexdigest": "4a4457435d58dd588483e6fdbdfef737", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002246AD21", "query_cov": 0.8678414096916299, "subject_cov": 0.8565217391304348, "identity": 0.995, "score": 377.0, "evalue": 6.06e-131, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "GIHJHMOALN_897", "locus": "GCA009939745_00882" }, { "type": "cds", "sequence": "contig_1", "start": 497965, "stop": 498420, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAAAAAGAAAAAAACTGCTCCAGGGTTTCTTGAAATTTTTACCGCTTATCATTCCCGCAACCATTTTTGTTAGCTGTGCAAGACGTGAAAGTAATCACCTCATCTTCAACATTTCACTCGATCACGACGCTGATGCTTCTATTAGTAAGTTCTTTGAACTTTACTCTAATAATTTAAGTAAAAAACTAGATAAAAAGGTTACCGTTAGCTTCAACATTATTGATGACTCTTTTACCAAAATTAGCAACATTCAAACTGCTAAAGCTGACTTTGCTTTCGTTAATTCTCAAAGTATTAAAGATAACGGAATAGAAGGATTTAATCTTATTCTTCAAACTCAAACTGATGCATTTAAGGAAGACACCAATTTAGATTATTACAGTGACGGTCAGTTGAAATCTAAAGCAGAAAAAATGACCACTTTATTTTCAAAAACGCCATATAAAGATTGA", "aa": "MLKRKKLLQGFLKFLPLIIPATIFVSCARRESNHLIFNISLDHDADASISKFFELYSNNLSKKLDKKVTVSFNIIDDSFTKISNIQTAKADFAFVNSQSIKDNGIEGFNLILQTQTDAFKEDTNLDYYSDGQLKSKAEKMTTLFSKTPYKD", "aa_hexdigest": "5c58614fea87685bf30c1110ff3049d5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17205.491699999977, "isoelectric_point": 8.887885475158694 }, "id": "GIHJHMOALN_898", "locus": "GCA009939745_00883" }, { "type": "cds", "sequence": "contig_1", "start": 498580, "stop": 498792, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTATGGCATCGGTCATGGCTCAAGTGGTTCAGCTGGAAAGTTCCAACTACCTGATCTTTTATTAAGAAAGCACTTTGGCTCTAGTTACCCCGGTTTGCAAAATGCAATTAACCAAAACCCTGATAAGTTTGCTAATGTTAGAGGTCGAGAAATAGGTAGAGACAATAAAATCAAAATTGTCTTTGATGATGCTAATTCCTTTGCTTGA", "aa": "MNYGIGHGSSGSAGKFQLPDLLLRKHFGSSYPGLQNAINQNPDKFANVRGREIGRDNKIKIVFDDANSFA", "aa_hexdigest": "469fe2f99e433fdb535c27c8a25b915a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7651.465000000001, "isoelectric_point": 9.598198127746581 }, "id": "GIHJHMOALN_899", "locus": "GCA009939745_00884" }, { "type": "cds", "sequence": "contig_1", "start": 498916, "stop": 499107, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTATGACATTGGCATTGTAAGTGATACTTTAAGTGATCGCTATCAAAAAGCAATTGCTGAAGTTTTTGTGGAACTAGCTAAAACAAAACAAGATATTTATGGCCCTTCATATGGTTACAACGGTTACAATTTGATTACGGACCCTAACAAAGAAATCTTGGATGTAATTCATAAAACATATGGTTAA", "aa": "MLYDIGIVSDTLSDRYQKAIAEVFVELAKTKQDIYGPSYGYNGYNLITDPNKEILDVIHKTYG", "aa_hexdigest": "d5362b939a7c09d9e9b55ef7033bf097", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7161.999900000001, "isoelectric_point": 4.749375343322753 }, "id": "GIHJHMOALN_900", "locus": "GCA009939745_00885" }, { "type": "cds", "sequence": "contig_1", "start": 499100, "stop": 499402, "strand": "+", "frame": 2, "gene": "p29", "product": "putative ABC transporter ATP-binding protein p29", "db_xrefs": [ "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47532", "UniRef:UniRef90_P75370" ], "nt": "ATGGTTAATCCAGTCTTAGTTTTTGATCAAGTTTCCCTTAAATATAACGGAGCACCACTTTTAGAAAACATTAACTTTACAATTTCACCTGGTGAACACATTTGCTTGTTGGGTAAATCTGGTGTTGGTAAAACTAGTCTTTTAAACTGCATTACTAACACCAAAACAATTTCGAAGGGAACCATTTATTTCAATGGTATTGCTTCTAATAACAAGGATTACAAACAATTAAAAAAACAGTTCAGTTTTTTAGATCAAGTACCTAATTTAATCGACACTGACTTCGTTTATGACGCCATTTGA", "aa": "MVNPVLVFDQVSLKYNGAPLLENINFTISPGEHICLLGKSGVGKTSLLNCITNTKTISKGTIYFNGIASNNKDYKQLKKQFSFLDQVPNLIDTDFVYDAI", "aa_hexdigest": "d724ed711a36eab42415198643243f55", "start_type": "ATG", "rbs_motif": null, "genes": [ "p29" ], "truncated": "3-prime", "pseudogene": { "start": 499100, "stop": 499705, "observations": { "mutation": [ 499400, 499430 ], "stop": [ 499400, 499430 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75370", "query_cov": 1.0, "subject_cov": 0.4098360655737705, "identity": 0.99, "score": 202.0, "evalue": 6.86e-64, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MVNPVLVFDQVSLRYNGAPLLENINFTISPGEHICLLGKSGVGKTSLLNCITNTKTISKGTIYFNGIASNNKDYKQLKKQFSFLDQVPNLIDTDFVYDAIWREAKNNLKWWQRLFLVEPQSLREQIIQILEEVNLKEYVTYIIKDLSGGQKQRVEVAKLFFANSQVLLVDEPTTGLDLINAHKIMELIIQFARQKAMTLIFVTHDVEFALKYSDRIIALKNKALVLDQATNKLTKQKLMQIYHD" }, "paralog": false, "identity": 0.9800995024875622, "score": 386.0, "evalue": 9.89e-139, "description": "Internal stop codon at 499400, 499430. Nonsense mutation around 499400, 499430.", "psc": { "uniref90_id": "UniRef90_P75370", "gene": "p29", "product": "Probable ABC transporter ATP-binding protein p29", "uniref50_id": "UniRef50_P47532", "cog_id": "COG1120", "cog_category": "PH", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75370", "UniRef:UniRef50_P47532", "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47532" ], "product": "Probable ABC transporter ATP-binding protein p29" } }, "id": "GIHJHMOALN_901", "locus": "GCA009939745_00886" }, { "type": "cds", "sequence": "contig_1", "start": 499448, "stop": 499834, "strand": "+", "frame": 2, "gene": "p29", "product": "putative ABC transporter ATP-binding protein p29", "db_xrefs": [ "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47532", "UniRef:UniRef90_P75370" ], "nt": "GTGGAACCTCAATCGCTAAGAGAACAAATTATCCAAATCCTTGAAGAGGTTAACCTAAAGGAATATGTCACTTACATTATTAAGGACTTGTCAGGTGGTCAAAAACAACGTGTGGAAGTAGCTAAATTGTTCTTTGCCAATTCCCAAGTTTTATTAGTTGATGAGCCCACTACTGGACTTGATTTAATCAATGCGCACAAAATCATGGAACTAATTACCCAATTTGCGCGCCAAAAAGCAATGACCTTAATCTTTGTGACCCATGATGTTGAGTTTGCGCTCAAATACAGTGACCGTATTATTGCTCTAAAAAATAAGGCATTAGTTTTAGATCAAGCTACAAATAAGCTTACCAAACAAAAGTTAATGCAAATTTATCATGACTAG", "aa": "MEPQSLREQIIQILEEVNLKEYVTYIIKDLSGGQKQRVEVAKLFFANSQVLLVDEPTTGLDLINAHKIMELITQFARQKAMTLIFVTHDVEFALKYSDRIIALKNKALVLDQATNKLTKQKLMQIYHD", "aa_hexdigest": "35b12e31d8b5d7238a7513f96e01fa03", "start_type": "GTG", "rbs_motif": null, "genes": [ "p29" ], "truncated": "5-prime", "pseudogene": { "start": 499148, "stop": 499834, "observations": { "mutation": [ 499400, 499430 ], "stop": [ 499400, 499430 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75370", "query_cov": 1.0, "subject_cov": 0.5245901639344263, "identity": 0.9840000000000001, "score": 239.0, "evalue": 2.81e-78, "gene_start": 1, "gene_end": 128, "reference_start": 117, "reference_end": 244, "reference_sequence": "MVNPVLVFDQVSLRYNGAPLLENINFTISPGEHICLLGKSGVGKTSLLNCITNTKTISKGTIYFNGIASNNKDYKQLKKQFSFLDQVPNLIDTDFVYDAIWREAKNNLKWWQRLFLVEPQSLREQIIQILEEVNLKEYVTYIIKDLSGGQKQRVEVAKLFFANSQVLLVDEPTTGLDLINAHKIMELIIQFARQKAMTLIFVTHDVEFALKYSDRIIALKNKALVLDQATNKLTKQKLMQIYHD" }, "paralog": false, "identity": 0.9868421052631579, "score": 439.0, "evalue": 4.08e-159, "description": "Internal stop codon at 499400, 499430. Nonsense mutation around 499400, 499430.", "psc": { "uniref90_id": "UniRef90_P75370", "gene": "p29", "product": "Probable ABC transporter ATP-binding protein p29", "uniref50_id": "UniRef50_P47532", "cog_id": "COG1120", "cog_category": "PH", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75370", "UniRef:UniRef50_P47532", "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47532" ], "product": "Probable ABC transporter ATP-binding protein p29" } }, "id": "GIHJHMOALN_902", "locus": "GCA009939745_00887" }, { "type": "cds", "sequence": "contig_1", "start": 500031, "stop": 500273, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCTCTTACCTACTGCAAACCATTTTTTATGTCGTGAGTGGTAGTATTTTGGGATTTGTCATAGCCCTGTGGTTTTCGTACCTCACTGCCTTTAAAATCCAGCCACTTTACATCGCTTTACCAACTCGCTTATTCACCATTTTTCTGCGTTCCTTTCCAGTACTTGTGTTTGCTTTTTTGTTTAACAATTTGTTTAACAAACAACTAACAGCAACCTTAACGATTACCTGGTTTAGTTGA", "aa": "MGSYLLQTIFYVVSGSILGFVIALWFSYLTAFKIQPLYIALPTRLFTIFLRSFPVLVFAFLFNNLFNKQLTATLTITWFS", "aa_hexdigest": "3275dc4062964590309d04ffad3c665b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9256.969499999988, "isoelectric_point": 9.994614219665529 }, "id": "GIHJHMOALN_903", "locus": "GCA009939745_00888" }, { "type": "cds", "sequence": "contig_1", "start": 500460, "stop": 500678, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTATTGCTGGCATTACTGGAATTGGTGAGCTCATTGCCACACCTCTAGGGGGCACAGTGCAGCTTAACTTAGTTTTAATACCGATGTTAACTTTGATCGGCTTTTTACTGTTTTTAGAAGCGAGTGTTTTTCTGTTAACTAAGTTTGTTTTACAAAAACAGAGCCAAGCCGGTGATTACTTTTTACAAGCTAAAACACTGCAAAAGAGAAAGTGA", "aa": "MSIAGITGIGELIATPLGGTVQLNLVLIPMLTLIGFLLFLEASVFLLTKFVLQKQSQAGDYFLQAKTLQKRK", "aa_hexdigest": "f988381b71b2440f54ca07680c3befa8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7822.357799999995, "isoelectric_point": 9.69799518585205 }, "id": "GIHJHMOALN_904", "locus": "GCA009939745_00889" }, { "type": "cds", "sequence": "contig_1", "start": 500688, "stop": 501191, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTACATCCTAGCTTTGGTTTTAGCGGCTTTTACCTTGGCTAATTTAGTACAGTTGGACTATACGGTTAAAGCGCCAGGGTTTGTGGCTGACTTTTTTAAACAGTTCTTTCAAACAAAAACAGCTTTTTTAATTAGTGAAGACGCTAACATTAACCCACTTTTAATGTTGTTAAAACTCACAACTCAAGCGATTAGTTTAATTACCTTAGTGTTTGTGTTAGCACTCTTGTTTGGCTTTTTAGCATCAAAGCTGTTTTCCACTATAACCTCAATTAGCTTAAAACTGTTACTTTTAGTGATTAGGGTAATTCCCAGTGTCTTACTGTTCCGCTTATTTGATCCAATTATTTTTCGCCCAGAAACTACCATTATCTTTGTTTTAGCAATCCACAGTGCTGCTAGTTATGGTCAACTGATAACGATTAACTTTGATAATGCCAACGAAGGGGTAATTAACAACATGCAGAACCACGGCTTTAGTAGGTTTTACATCTTGTGA", "aa": "MIYILALVLAAFTLANLVQLDYTVKAPGFVADFFKQFFQTKTAFLISEDANINPLLMLLKLTTQAISLITLVFVLALLFGFLASKLFSTITSISLKLLLLVIRVIPSVLLFRLFDPIIFRPETTIIFVLAIHSAASYGQLITINFDNANEGVINNMQNHGFSRFYIL", "aa_hexdigest": "af800cc8c3311ccb8242ea5532686aef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18696.141499999987, "isoelectric_point": 9.048734092712401 }, "id": "GIHJHMOALN_905", "locus": "GCA009939745_00890" }, { "type": "cds", "sequence": "contig_1", "start": 501273, "stop": 501437, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGGGATCTTTGGTGGTTCGATTATTGGGGGGCGAATTAACAATTTCTTTGAGCGAGCACAGTACTCTGAATTGGGCACAATAACCTTACCCTTAATGGTTTACTTAATGGTGTTTGAAGTCATTTTAATGGCAGTACGGTTAAACAAACTCAAAGTGTGA", "aa": "MFGIFGGSIIGGRINNFFERAQYSELGTITLPLMVYLMVFEVILMAVRLNKLKV", "aa_hexdigest": "358558c19e8255f9d64aafba5048e67b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6112.340400000001, "isoelectric_point": 9.518902015686034 }, "id": "GIHJHMOALN_906", "locus": "GCA009939745_00891" }, { "type": "cds", "sequence": "contig_1", "start": 501456, "stop": 501878, "strand": "-", "frame": 2, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "COG:COG0816", "COG:JLK", "EC:3.1.-.-", "EC:3.1.21.10", "GO:0000967", "GO:0004518", "GO:0005829", "GO:0006364", "RefSeq:WP_014325523.1", "SO:0001217", "UniParc:UPI0001DD63E0", "UniRef:UniRef100_A0AB33HMQ4", "UniRef:UniRef50_Q9ZB76", "UniRef:UniRef90_P57114" ], "nt": "GTGCAGTACATTTTAGGCATTGATTTTGGTTTGAAGAGAATTGGTACAGCACTAGCCAATACCATTGACCGCTTTCCAAGTCCCTTCCGCGTGTTTGCGGTACAAAATAATTTGCAACAAGCCGTTAATACCCTTTTTAAGGATTTAAAGCAAGCTGGTTATGAATTAGTGCAAATAGTGATTGGCTTTCCCCACTTCCACTACCAGTCAAGTATCCAAGTGAGCATTCACAAGTTTGTGGAACTAATAAAAACCCGCTTTAATGTACCAGTAACACTCATTGATGAAAGTGGTACTACGAGTGAGGTAAAAGCTAACTTGCAGGAATTAGGCTTAAAAAACAGAACCTTTAAAAAGGCCAAGGACACCTTAGCGGCTACCTTAATCTTAGAGCGTTTCTTAAACCAACAAAAACCCAATTAA", "aa": "MQYILGIDFGLKRIGTALANTIDRFPSPFRVFAVQNNLQQAVNTLFKDLKQAGYELVQIVIGFPHFHYQSSIQVSIHKFVELIKTRFNVPVTLIDESGTTSEVKANLQELGLKNRTFKKAKDTLAATLILERFLNQQKPN", "aa_hexdigest": "fd720376636ad9189676bc3c1a3257ed", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63E0", "ncbi_nrp_id": "WP_014325523.1", "uniref100_id": "UniRef100_A0AB33HMQ4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63E0", "RefSeq:WP_014325523.1", "UniRef:UniRef100_A0AB33HMQ4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HMQ4", "uniref90_id": "UniRef90_P57114" }, "psc": { "uniref90_id": "UniRef90_P57114", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.-.-", "3.1.21.10" ], "uniref50_id": "UniRef50_Q9ZB76", "cog_id": "COG0816", "cog_category": "JLK", "go_ids": [ "GO:0000967", "GO:0004518", "GO:0005829", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB76" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "GIHJHMOALN_907", "locus": "GCA009939745_00892" }, { "type": "cds", "sequence": "contig_1", "start": 501878, "stop": 502366, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACAAGCAAAAAACTGCTTTAACTAAGCAACTAAATCACTTTCACTTGCCGCAAGTAATTACTGATTTACGTAAATGCCAAGCTTTACTAAATGAACTCAAAATAACGGTTAATGAACTGAAAACGGAAGACTTTAAGTGGAAGCAAAAGCAGTTAGCGGAAAAGATCAAACAGGAATTGTTAGAGCTAGCAAAGCAAGACAAAGCTTATGTTCTCGCTAGCTTTGCAGCAGTTGATCCGAAACTGTTATCACAAGTAGCCCAAGCAGTCTTGAACCAACACAAAAACAAGCTGTTTGTACTCCTCAACCAATTCAACAACAGTCCGTCCTTTATGTTATTAGGGCAAGATGTTTCTAAATGTATTCAGCTTTTAAAAGCTCACTTTGAACTTAAGGGTGGGGGTAGTAATAACTTCTTTAGGGGTTCGTTTAACGAGTCGGTGGATGTAAGTAAACTACAAGCTATCCTCGATACCCTTCAATAG", "aa": "MNKQKTALTKQLNHFHLPQVITDLRKCQALLNELKITVNELKTEDFKWKQKQLAEKIKQELLELAKQDKAYVLASFAAVDPKLLSQVAQAVLNQHKNKLFVLLNQFNNSPSFMLLGQDVSKCIQLLKAHFELKGGGSNNFFRGSFNESVDVSKLQAILDTLQ", "aa_hexdigest": "858125cfe8d0ecc94c0f72852f2ab4f4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18422.22019999999, "isoelectric_point": 9.439734840393069 }, "id": "GIHJHMOALN_908", "locus": "GCA009939745_00893" }, { "type": "cds", "sequence": "contig_1", "start": 502487, "stop": 502738, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "GTGGAACACAGGATTCGACAAATTATTCAACAAGACATTCAGCGCGAAGAGATCTTTACTGATCTAGAGGGTAGTCAAAAACTTAATGCGATTGCCTACTTTGAAGAGGAGTATTCGAACCACGAGTTGTTACGGGTAATTCGCTTTGGTGATTTTAGTGTCGAGCTTTGTGGTGGTACCCATGTGGAACACACTGGTTTAATTGAGAACTGTTTTATTACTGATTACTATGCTCGTGGCACAGGGCGTTGA", "aa": "MEHRIRQIIQQDIQREEIFTDLEGSQKLNAIAYFEEEYSNHELLRVIRFGDFSVELCGGTHVEHTGLIENCFITDYYARGTGR", "aa_hexdigest": "906ac5d966814cf2bbcd1d4b368bde07", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 37, "aa_cov": 0.4457831325301205, "hmm_cov": 1.0, "evalue": 5.1037164031738117e-14, "score": 47.535125732421875, "start": 44, "stop": 80 } ], "seq_stats": { "molecular_weight": 9751.746899999991, "isoelectric_point": 4.915629386901855 }, "id": "GIHJHMOALN_909", "locus": "GCA009939745_00894" }, { "type": "cds", "sequence": "contig_1", "start": 502754, "stop": 503299, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAGCACCGTTTGATCTCCAAACAAAACAGTAATACGTTGAGCTTTGAAAAGCAAAATGAACACTTAGTGAACTTTAAAACAGCGAGTGAGTTCTTATACGAGGCTAATGAGATCACCGCCAAGGTCATTGGGCTGTTTGATGAACAGTACCAACCGGTGCAAAAACTGCACAACCAAAGTGGTTATGTGGTGTTTGACCAAACGGTGCTGTATGCCACATCAGGCGGTCAGCGTTATGATGAGGGGTACTGTATTAACCACAGCCAAAATGATCAAAGAGTAAGCTTTCAGGGCGTGTTTAAAGGCCCTAATAAACAACACTTCCACTTCTTTTTAACTGGTAGTTTTCAACTAGGTGACAAAGTGATCCTAGTACATGACGGTAAGTGGCGCCAACTAGTGAAAAACAACCACAGTTTAGAACACTTACTGCACGCGGCATTGCAAAAGGAAATTGACCCCTTGATTAAACAAGACGGCGCCTTTAAGTCAGCGCAAAAAGCTACCATTGACTTTAATTTTTCGCGAGCCTTAACCTGA", "aa": "MEQHRLISKQNSNTLSFEKQNEHLVNFKTASEFLYEANEITAKVIGLFDEQYQPVQKLHNQSGYVVFDQTVLYATSGGQRYDEGYCINHSQNDQRVSFQGVFKGPNKQHFHFFLTGSFQLGDKVILVHDGKWRQLVKNNHSLEHLLHAALQKEIDPLIKQDGAFKSAQKATIDFNFSRALT", "aa_hexdigest": "b183fc40ff4877e61fecd2dd9fcfc333", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20830.082300000013, "isoelectric_point": 7.165941810607911 }, "id": "GIHJHMOALN_910", "locus": "GCA009939745_00895" }, { "type": "cds", "sequence": "contig_1", "start": 503318, "stop": 503449, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTTTGAAAAGAGTATTGCTCATAATGCACTCAAACCCCAAGTAGCCTTCCAGTTGTACGAAACCTATGGCTTTCCGATTGAAATGATTAAGGAACTGGTGGATAAGCGCCAACTCCAGGTAGACTGA", "aa": "MLFEKSIAHNALKPQVAFQLYETYGFPIEMIKELVDKRQLQVD", "aa_hexdigest": "ea8e8dd49d402ee4e2404e79108d020f", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5082.889900000002, "isoelectric_point": 5.648738670349121 }, "id": "GIHJHMOALN_911", "locus": "GCA009939745_00896" }, { "type": "cds", "sequence": "contig_1", "start": 503492, "stop": 503989, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAGTGTGGAACATTGTCTTTAGTCAGTTTAACAATGATGGCAATGGTAACTACAGTGAGTTAGCGCAGAAGAACATCGATACTGGGGCCGGTATTGAACGCCTAGTAGCAATCTTACAGGACGCGCCGACTAACTTTGACACGGATATCTTCTTAAAGTTAATTGGCATTATTGAACAGCACTGTAAGCATAAGTATGATACCAACTTATACTTTAAGTTTGACCAAAAGTTAAACGAAGCGCAAAGTGCCTTTCGCATTATTAGTGACCACTTTAAGGCAATTACCTTCACCATTGCCGAAGGCGTATTGCCAGGACCCAACGAGCGCAGTTACATTGTGCGCCGGTTACTGCGCCGCGCTTTGTTGGCCTGCAAAAAACTGGACTTAGACTTAAAGTTTATTGATCCGATGGTCGATGCCATTATTAGCGTTTATGGGTCTTACTACCAGCAGTTGCAGGGGAAAAACCAGGTGGTGCAACAAGCAATCTGA", "aa": "MEVWNIVFSQFNNDGNGNYSELAQKNIDTGAGIERLVAILQDAPTNFDTDIFLKLIGIIEQHCKHKYDTNLYFKFDQKLNEAQSAFRIISDHFKAITFTIAEGVLPGPNERSYIVRRLLRRALLACKKLDLDLKFIDPMVDAIISVYGSYYQQLQGKNQVVQQAI", "aa_hexdigest": "b6913b4e3ad5fad4030215ddddc32e9e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18876.393899999977, "isoelectric_point": 6.283743858337403 }, "id": "GIHJHMOALN_912", "locus": "GCA009939745_00897" }, { "type": "cds", "sequence": "contig_1", "start": 504170, "stop": 504316, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGGTAACTTCTCGATTGATGATTACTTTAAGGAAGAGGCCATTGGCTTTGCCAATGATCTTTTAGTCAATCATTACCACTTGGATCCGAAGCGCTTTTACATTACAGTGTACCAAGATGATGAATTGACCTTTAACACCTGA", "aa": "MLGNFSIDDYFKEEAIGFANDLLVNHYHLDPKRFYITVYQDDELTFNT", "aa_hexdigest": "b36c6a83d323a92b7010809069bd0133", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5734.2734, "isoelectric_point": 4.304610252380372 }, "id": "GIHJHMOALN_913", "locus": "GCA009939745_00898" }, { "type": "cds", "sequence": "contig_1", "start": 504851, "stop": 505357, "strand": "-", "frame": 3, "gene": "ugpQ", "product": "putative MG293-like protein", "db_xrefs": [ "COG:COG0584", "COG:I", "GO:0006629", "GO:0008081", "SO:0001217", "UniRef:UniRef50_P75367", "UniRef:UniRef90_P75367" ], "nt": "ATGCTTAAACGACAACTTCTGCTAGCACACCGTGGGTACAGTGATATTGCGCCTGAAAACACCCAGCTAGCGTTTGAATTAGCGTTCCAATATCGCTTTGATGGCGTGGAGTTAGATGTGCACTTGACCAAGGACGGGGAGTTAGTGATTATCCATGATGAAACTACCACCAGAACAGCGCTAGTCGACAAAACAATCGAACTGGAAACACTCGCTAGTTTAAAGCAAGATGATCACAGTGCTTTCTTTAAGTTCAAAACGCAACCCCAGCCGATTATGACCTTAAAGGAGTTCTTTGATCAGTACTTAGATAAATTTCAACTAATTAACGTGGAAATTAAAACTGACCAAAAGGAATACCCGGGAATTGAAGCTAAAATTGATGCTTTGGCCCAACAGTATGGCAAAAAAGTAATTGAAAAGGTAGTGTTCTCCTCGTTTAACTTTGCTTCCTTACAGCGCTTGTATGACATTAACCCCAACTACCAAATAGCCTTTTTGTTTTGA", "aa": "MLKRQLLLAHRGYSDIAPENTQLAFELAFQYRFDGVELDVHLTKDGELVIIHDETTTRTALVDKTIELETLASLKQDDHSAFFKFKTQPQPIMTLKEFFDQYLDKFQLINVEIKTDQKEYPGIEAKIDALAQQYGKKVIEKVVFSSFNFASLQRLYDINPNYQIAFLF", "aa_hexdigest": "9150a4b1420c7ec40b6892eac91394d1", "start_type": "ATG", "rbs_motif": null, "genes": [ "ugpQ" ], "truncated": "5-prime", "pseudogene": { "start": 504632, "stop": 505357, "observations": { "mutation": [ 504721, 504784, 504853 ], "stop": [ 504721, 504784, 504853 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75367", "query_cov": 1.0, "subject_cov": 0.6970954356846473, "identity": 1.0, "score": 330.0, "evalue": 2.23e-113, "gene_start": 1, "gene_end": 168, "reference_start": 1, "reference_end": 168, "reference_sequence": "MLKRQLLLAHRGYSDIAPENTQLAFELAFQYRFDGVELDVHLTKDGELVIIHDETTTRTALVDKTIELETLASLKQDDHSAFFKFKTQPQPIMTLKEFFDQYLDKFQLINVEIKTDQKEYPGIEAKIDALAQQYGKKVIEKVVFSSFNFASLQRLYDINPNYQIAFLFWTKKQFQAVDALKIKQVCQYLHPWTNIYEKFPDMVLSLQLPLGLWTLNSEVKFHQFRQDRMVYAQIANKKFEV" }, "paralog": false, "identity": 0.9875518672199171, "score": 468.0, "evalue": 4.56e-170, "description": "Internal stop codon at 504721, 504784, 504853. Nonsense mutation around 504721, 504784, 504853.", "psc": { "uniref90_id": "UniRef90_P75367", "gene": "UgpQ", "product": "Uncharacterized protein MG293 homolog", "uniref50_id": "UniRef50_P75367", "cog_id": "COG0584", "cog_category": "I", "go_ids": [ "GO:0008081", "GO:0006629" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75367", "UniRef:UniRef50_P75367", "COG:COG0584", "COG:I", "GO:0008081", "GO:0006629" ] }, "pscc": { "uniref50_id": "UniRef50_P75367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75367" ], "product": "Uncharacterized protein MG293 homolog" } }, "id": "GIHJHMOALN_914", "locus": "GCA009939745_00899" }, { "type": "cds", "sequence": "contig_1", "start": 505407, "stop": 505997, "strand": "-", "frame": 2, "gene": null, "product": "putative MG294-like protein", "db_xrefs": [ "GO:0022857", "KEGG:K26602", "SO:0001217", "UniRef:UniRef50_P75366", "UniRef:UniRef90_P75366" ], "nt": "ATGGGAATGTATGTCTTTCAAAGTACGTTTGCTTATTATCTTTTACAAATGATGCAAAACGCCTTTTTGGCACCAGTGATCTTAGGGACTGTACTGGGTGGTGTAAGGACGTATGTTTTGCGCAGTGCTGTAAGTGTGTACTTGGGCAGGTTAGCTGATAAGTTTAGGAGCTACATCCTCTTTTTAATGTTGTGTACAGGACTTGGCATTATCTTTGTCTTGATGTTTATTCTCTTAGGTTTTGGGCAAGTGGGCCAACAGCAAAACTATGCCTTGATTATCGTATCGGCGATCCTCTACATTCTCACCGGGGTGTTGTCTTGGGGTATGGTAACCGTTAGGTACAACCAGGTAGCGGAAATAGAGATTGGTAAAAACAACTATGCTTCTAGTGTTGGTTTACTTTCCTTTATTGGTTTTTCGACTGATGGTTGGCTCTACACGGTTACAAGTGTCGTGGGTAAGGCTTATACCCCAGACGGTCAGAAGAATACCTCGATTCAGGGTTACCAAATTATTGCAGCTATTTGTCTAGGAATAGCCCTGTTTGGTTTATTGTGTGGTACGATTGTCTTTTTGGTTAATACTTGA", "aa": "MGMYVFQSTFAYYLLQMMQNAFLAPVILGTVLGGVRTYVLRSAVSVYLGRLADKFRSYILFLMLCTGLGIIFVLMFILLGFGQVGQQQNYALIIVSAILYILTGVLSWGMVTVRYNQVAEIEIGKNNYASSVGLLSFIGFSTDGWLYTVTSVVGKAYTPDGQKNTSIQGYQIIAAICLGIALFGLLCGTIVFLVNT", "aa_hexdigest": "d292932f2a7c8b84e976e4ff46349281", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 505350, "stop": 506297, "observations": { "mutation": [ 505409, 506018 ], "stop": [ 505409, 506018 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75366", "query_cov": 1.0, "subject_cov": 0.4126315789473684, "identity": 1.0, "score": 367.0, "evalue": 3.04e-124, "gene_start": 1, "gene_end": 196, "reference_start": 261, "reference_end": 456, "reference_sequence": "METKLSLKKRLQQFSKKQLIALIILGAADVFVIAAPYYIKNVVPNLHLYLGITEDEVATLTSIIGYVTLATQLPGGFLANRFSSRKLLFLSEISTGVITFWLATNILTRESQKSNALFVQYCVIWGLWGITSTLIFWTPLWKLASQQATQENQALGFGIQGAANGVWGFIFIFLIALIITAVAYPAGGESSANNPAPFAIYAFIIGGMLLVTGFTVLFFVPEKPIEKYDSHTSLKTAKKNFEQILITLKNWKLWLLSFFLMGMYVFQSTFAYYLLQMMQNAFLAPVILGTVLGGVRTYVLRSAVSVYLGRLADKFRSYILFLMLCTGLGIIFVLMFILLGFGQVGQQQNYALIIVSAILYILTGVLSWGMVTVRYNQVAEIEIGKNNYASSVGLLSFIGFSTDGWLYTVTSVVGKAYTPDGQKNTSIQGYQIIAAICLGIALFGLLCGTIVFLVNTWELKRLGKTDYRWRTLDNA" }, "paralog": false, "identity": 0.9936507936507937, "score": 615.0, "evalue": 7.02e-224, "description": "Internal stop codon at 505409, 506018. Nonsense mutation around 505409, 506018.", "psc": { "uniref90_id": "UniRef90_P75366", "product": "Uncharacterized protein MG294 homolog", "uniref50_id": "UniRef50_P75366", "kegg_orthology_id": "K26602", "go_ids": [ "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75366", "UniRef:UniRef50_P75366", "KEGG:K26602", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P75366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75366" ], "product": "Uncharacterized protein MG294 homolog" } }, "id": "GIHJHMOALN_915", "locus": "GCA009939745_00900" }, { "type": "cds", "sequence": "contig_1", "start": 506016, "stop": 506315, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTTTTGGTATTCAGGGCGCTGCTAATGGTGTTTGGGGTTTTATTTTTATTTTCTTAATTGCCTTGATTATTACAGCAGTAGCTTACCCAGCCGGTGGTGAAAGTAGTGCTAATAATCCCGCACCGTTTGCCATTTATGCCTTTATTATTGGTGGCATGTTGCTCGTTACTGGGTTTACCGTGCTGTTCTTTGTACCAGAAAAACCAATTGAAAAGTACGACAGTCATACCAGTTTAAAAACTGCCAAAAAGAACTTTGAACAGATTCTCATTACCTTAAAGAACTGGAAGTTGTGA", "aa": "MGFGIQGAANGVWGFIFIFLIALIITAVAYPAGGESSANNPAPFAIYAFIIGGMLLVTGFTVLFFVPEKPIEKYDSHTSLKTAKKNFEQILITLKNWKL", "aa_hexdigest": "63a585f44eef0f019808b1faa2201401", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10771.587999999987, "isoelectric_point": 9.050474739074708 }, "id": "GIHJHMOALN_916", "locus": "GCA009939745_00901" }, { "type": "cds", "sequence": "contig_1", "start": 506367, "stop": 506777, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACGAAACTTAGTTTAAAGAAACGGTTGCAGCAGTTTTCCAAAAAGCAGCTCATAGCACTAATTATTCTCGGTGCGGCTGATGTTTTTGTAATTGCGGCCCCGTACTATATTAAGAATGTGGTGCCTAACTTGCACCTGTATTTAGGGATTACTGAAGATGAAGTGGCCACCTTAACCTCGATTATTGGTTATGTGACCTTAGCCACGCAGTTACCTGGTGGTTTTTTAGCGAACCGGTTTAGTTCGCGGAAACTATTGTTCTTGTCAGAAATTTCAACGGGTGTAATTACCTTTTGGCTAGCTACTAACATTCTTACTCGTGAAAGCCAAAAATCCAATGCCTTGTTTGTACAGTACTGTGTTATTTGGGGGCTTTGGGGTATTACTTCGACCTTAATCTTTTGA", "aa": "METKLSLKKRLQQFSKKQLIALIILGAADVFVIAAPYYIKNVVPNLHLYLGITEDEVATLTSIIGYVTLATQLPGGFLANRFSSRKLLFLSEISTGVITFWLATNILTRESQKSNALFVQYCVIWGLWGITSTLIF", "aa_hexdigest": "1e1de476bf12f7f72e44b0cc1490fdea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15201.783599999988, "isoelectric_point": 9.518063926696776 }, "id": "GIHJHMOALN_917", "locus": "GCA009939745_00902" }, { "type": "cds", "sequence": "contig_1", "start": 506908, "stop": 507189, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20258.4" ], "nt": "TTGTATGTCTCGAGTGACCCAGAAGATTTACAGCGTCGTGGCATTACCCTTTCCCACTTTAACTGGTTGTACCAACCCAATCCATTGACCCAAACCGTTCTGGTACGGATTCGCCATGCCCAACCGTTAGTGCAAGGGCACATAACAGTACAACCAAACAACGTCGTGCAAGTACAACTAGATCAACCAATTGACCGCGTTACCAACGGTCAGTATGGTGTTTTGTATACCCAGAATGGCATTTGTTTGGGGAGTGGTATCATTACCGCTAGCCAAATCTAA", "aa": "MYVSSDPEDLQRRGITLSHFNWLYQPNPLTQTVLVRIRHAQPLVQGHITVQPNNVVQVQLDQPIDRVTNGQYGVLYTQNGICLGSGIITASQI", "aa_hexdigest": "dce2dc16aa520893f5e25662d3119ee3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20258.4", "name": "tRNA_Me_trans_C", "length": 77, "aa_cov": 0.8172043010752689, "hmm_cov": 0.987012987012987, "evalue": 2.5735918424724424e-25, "score": 84.11976623535156, "start": 13, "stop": 88 } ], "seq_stats": { "molecular_weight": 10424.715799999996, "isoelectric_point": 6.807856178283691 }, "id": "GIHJHMOALN_918", "locus": "GCA009939745_00903" }, { "type": "cds", "sequence": "contig_1", "start": 507319, "stop": 507693, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03054.22" ], "nt": "ATGTTGTGTAACCGTTTAATTAAATTTGGGCTAATGCGTGATTACTGCAAGCAGTTAGATCCCAACAGTGACTTTGCTACAGGGCATTATGCGGCACTTAGCTGGGACAACAACCAACCTTTACTGGCCATTCCCAAAGACAAGCACAAGGACCAAACCTATTTTTTAGCGCATGTCAAACCAGCGCAACTACAAGACGTGGTGTTTCCCTTGGCGCATTTACTGAAAACGGAGGTGCGGCAAATAGCGTTAGCACACCACTTTAGTGTGGCTACTAAAAAGGACTCAACCGGCATTTGCTTTATTGGGGAACGCCACTTTAGTGACTTCTTAAAGAATTATTTACCAGTCAAACCGGGGGTTATTTATGATTGA", "aa": "MLCNRLIKFGLMRDYCKQLDPNSDFATGHYAALSWDNNQPLLAIPKDKHKDQTYFLAHVKPAQLQDVVFPLAHLLKTEVRQIALAHHFSVATKKDSTGICFIGERHFSDFLKNYLPVKPGVIYD", "aa_hexdigest": "ba9d228296723ef3e820f3960e70ff9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 106, "aa_cov": 0.8387096774193549, "hmm_cov": 0.9905660377358491, "evalue": 1.5215725419431024e-38, "score": 127.94937133789062, "start": 1, "stop": 104 } ], "seq_stats": { "molecular_weight": 14199.280199999983, "isoelectric_point": 8.776741981506348 }, "id": "GIHJHMOALN_919", "locus": "GCA009939745_00904" }, { "type": "cds", "sequence": "contig_1", "start": 507754, "stop": 508020, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGCAAAATAAAACCGTTTTTGTCGGAATATCAGGTGGAGTGGACTCAGCTGTGAGTGCGCTCCTTTTAAAGCAACAGTACCGCGAAGTGATTGGCATTTTTATGGAGTGCTGGGACAATACACTAAATAACGACCAGTTAGGTCACCGCGCCTTTAACGAGCATAAGTCGGGTTGTTCGTCCAAAGAAGATTTTCGCGAAGCGCAAGCAATTGCCCAATTACTAGGAATCAAACTAATTAAGCAAAACCTGGTGGAACCGTACTGA", "aa": "MQNKTVFVGISGGVDSAVSALLLKQQYREVIGIFMECWDNTLNNDQLGHRAFNEHKSGCSSKEDFREAQAIAQLLGIKLIKQNLVEPY", "aa_hexdigest": "1932614864dae796562766c5f9c6253f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 82, "aa_cov": 0.9318181818181818, "hmm_cov": 0.7804878048780488, "evalue": 6.434436834393287e-08, "score": 27.460763931274414, "start": 3, "stop": 84 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 85, "aa_cov": 0.9659090909090909, "hmm_cov": 0.8941176470588236, "evalue": 2.0276479412517708e-22, "score": 75.30052185058594, "start": 4, "stop": 88 } ], "seq_stats": { "molecular_weight": 9856.130999999994, "isoelectric_point": 6.052977561950684 }, "id": "GIHJHMOALN_920", "locus": "GCA009939745_00905" }, { "type": "cds", "sequence": "contig_1", "start": 508021, "stop": 508410, "strand": "-", "frame": 1, "gene": null, "product": "putative MG296-like protein", "db_xrefs": [ "RefSeq:WP_010874779.1", "SO:0001217", "UniParc:UPI00001395B3", "UniRef:UniRef100_P75364", "UniRef:UniRef50_P75364", "UniRef:UniRef90_P75364" ], "nt": "ATGAAACCACAACTTTTAGCCTTGAAGCAGTTTGTGCAAACTGAGTTTGAAAAAGTTGATTTTGAGACCTTTCGCCAAAACTTTAATCGTTGTTTGGAACGCGAACAGAGTACCCTGTTAATTTATGAAGATGATGATTATGATGATCAGTCCTTCTTTTTAAAACCGATGCTCAGTGACGCCTTTTTTATTAGTAGTGAAGTGGTCAAACAACTGGATTTGTTAGCTGTTTTAGTGGACAATCCTAAAGGTGATGTCAAAAGTTGTTGTCAGAGCTTTTATGAAGCGTTAACCTTATTTATTTCAGCGCTGGCCATAACTAAGGGTGTTGATGTCGGGCGTTACCACCAGCAGTTGGGTAAGCGCTTTGGTGTTTTAACAGTTTACTAG", "aa": "MKPQLLALKQFVQTEFEKVDFETFRQNFNRCLEREQSTLLIYEDDDYDDQSFFLKPMLSDAFFISSEVVKQLDLLAVLVDNPKGDVKSCCQSFYEALTLFISALAITKGVDVGRYHQQLGKRFGVLTVY", "aa_hexdigest": "bd500a8f610e597845d6216cb1c1a641", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001395B3", "ncbi_nrp_id": "WP_010874779.1", "uniref100_id": "UniRef100_P75364", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001395B3", "RefSeq:WP_010874779.1", "UniRef:UniRef100_P75364" ] }, "ips": { "uniref100_id": "UniRef100_P75364", "uniref90_id": "UniRef90_P75364" }, "psc": { "uniref90_id": "UniRef90_P75364", "product": "Uncharacterized protein MG296 homolog", "uniref50_id": "UniRef50_P75364" }, "pscc": { "uniref50_id": "UniRef50_P75364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75364" ], "product": "Uncharacterized protein MG296 homolog" }, "genes": [], "id": "GIHJHMOALN_921", "locus": "GCA009939745_00906" }, { "type": "cds", "sequence": "contig_1", "start": 508410, "stop": 508718, "strand": "-", "frame": 2, "gene": "ylxM", "product": "YlxM family DNA-binding protein", "db_xrefs": [ "COG:COG2739", "COG:K", "GO:0048500", "KEGG:K09787", "RefSeq:WP_010874780.1", "SO:0001217", "UniParc:UPI000013A7F1", "UniRef:UniRef100_P75363", "UniRef:UniRef50_P75363", "UniRef:UniRef90_P75363" ], "nt": "ATGAACCAAACCAAACTTAACCAGCGTTTAAAGCAACAGCAACTATTTGATATTTACGGGGAACTTTTAACCAAACGGCAAGCGTGTTACTTTAACGAGTACATTAACCTGGATTTGAGCATGCAAGAGATTGCTGATAAATACCAAGTTAAGAAAAGTTCAATCCACCAACACATCAAAACCTGCAACCTGATCTTTAACCGTTTTGAAGCGAAATTGCAGTTGTTTAAAAAGCAACAGCTCCGTTTAAAGTTGTACGAAAAAATAACAGATCCCCAATTACGCGAGCAACTTATTAAACTAAGGTAA", "aa": "MNQTKLNQRLKQQQLFDIYGELLTKRQACYFNEYINLDLSMQEIADKYQVKKSSIHQHIKTCNLIFNRFEAKLQLFKKQQLRLKLYEKITDPQLREQLIKLR", "aa_hexdigest": "65aa099fec66c2c2366037bd6aea5dbc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013A7F1", "ncbi_nrp_id": "WP_010874780.1", "uniref100_id": "UniRef100_P75363", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013A7F1", "RefSeq:WP_010874780.1", "UniRef:UniRef100_P75363" ] }, "ips": { "uniref100_id": "UniRef100_P75363", "uniref90_id": "UniRef90_P75363" }, "psc": { "uniref90_id": "UniRef90_P75363", "gene": "ylxM", "product": "YlxM family DNA-binding protein", "uniref50_id": "UniRef50_P75363", "cog_id": "COG2739", "cog_category": "K", "kegg_orthology_id": "K09787", "go_ids": [ "GO:0048500" ] }, "pscc": { "uniref50_id": "UniRef50_P75363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75363" ], "product": "UPF0122 protein MPN_424" }, "genes": [ "ylxM" ], "id": "GIHJHMOALN_922", "locus": "GCA009939745_00907" }, { "type": "cds", "sequence": "contig_1", "start": 508699, "stop": 509130, "strand": "-", "frame": 1, "gene": null, "product": "Cell division protein FTSY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "nt": "GTGATCTTCCGTGGGGTGCAACAAGGCATTCAAAACGAATACGACTTTGTGTTGTGTGACACCTCGGGACGGTTGCAAAACAAAACCAACTTAATGAATGAGTTGAAAAAGATTTACCAAATTGTCCAGAAGGTGAGTAGCGCTAAACCCCAAGAAACACTGCTCGTTTTAGATGGTACTACCGGACAATCGGGGTTAGCACAGGCCAAGGTCTTTAATGAATTTACCGAGCTTACCGGGATTATCCTTACCAAAATGGACAGCTCCTCTAAGGGTGGGATTATCCTAGCGATTAAAGACCTGTTTAATTTACCGGTAAAACTGATTGGTTTTGGAGAAACAACTGCTGATTTAGCGGCCTTTGATTTAGAGCAATATGTACTGGGTCTTACCAAGAATTTAAGCCTCAACCATGAACCAAACCAAACTTAA", "aa": "MIFRGVQQGIQNEYDFVLCDTSGRLQNKTNLMNELKKIYQIVQKVSSAKPQETLLVLDGTTGQSGLAQAKVFNEFTELTGIILTKMDSSSKGGIILAIKDLFNLPVKLIGFGETTADLAAFDLEQYVLGLTKNLSLNHEPNQT", "aa_hexdigest": "7ec3940ce35358f300c5d2fe3e475f24", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PYR8", "query_cov": 0.8601398601398601, "subject_cov": 0.968503937007874, "identity": 0.675, "score": 169.0, "evalue": 1.15e-51, "valid": false, "product": "Cell division protein FTSY", "uniref50_id": "UniRef50_A0A3B0PYR8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PYR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "product": "Cell division protein FTSY" }, "genes": [], "id": "GIHJHMOALN_923", "locus": "GCA009939745_00908" }, { "type": "cds", "sequence": "contig_1", "start": 509188, "stop": 509745, "strand": "-", "frame": 1, "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "db_xrefs": [ "COG:COG0552", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006614", "GO:0009306", "GO:0016887", "KEGG:K03110", "SO:0001217", "UniRef:UniRef50_P75362", "UniRef:UniRef90_P75362" ], "nt": "ATGAGTTTTATCCACAAGCTAATCCAGAAGTTTAAACCGAAAAAGAAACTGGTCGACCAAGTGCAACAAGCTGTCCAGGAAAAGAGCTTCTTTCAAGCCAACCAAAAGAGCTATTACCAGGGTTTAAACAAGTCGGCTAACAGCTTTGCCAATACCATTAATAAACTAGCAGCTAACTATGTAACGGTTAACGAACAGTTTCAAGAAAGTTTGTTTGAGGAGTTAGTGTTGCTTGATATTGGTTACCATGCGGCTACTAAAATCTGTGATGCGATTGTCCAAGAACTCAAACTACAACGAGTGAGTGATCCTCAGTTAATCCAGGAAATTATTGTCGATAAGTTAATTGTTTACTATATCCAAGATAAGTTGTTTGAAACTGATTTAACGGTTGAAGCTAATAAAACCAACGTGTATTTGTTTGTCGGGGTTAATGGTGTTGGCAAAACAACTTCATTAGCAAAACTAGCTGACCAGCTTACCAAGCAAAACAAACGGGTGTTAATGGTCGCTGGGGATACCTTTCGCGCTGGGGCAGTGGCCCAGTTAGCAGAATGA", "aa": "MSFIHKLIQKFKPKKKLVDQVQQAVQEKSFFQANQKSYYQGLNKSANSFANTINKLAANYVTVNEQFQESLFEELVLLDIGYHAATKICDAIVQELKLQRVSDPQLIQEIIVDKLIVYYIQDKLFETDLTVEANKTNVYLFVGVNGVGKTTSLAKLADQLTKQNKRVLMVAGDTFRAGAVAQLAE", "aa_hexdigest": "72e09c2c86b35f85ac3ac3bab2b294cf", "start_type": "ATG", "rbs_motif": null, "genes": [ "ftsY" ], "truncated": "5-prime", "pseudogene": { "start": 508885, "stop": 509745, "observations": { "mutation": [ 509190 ], "stop": [ 509190 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75362", "query_cov": 1.0, "subject_cov": 0.5316091954022989, "identity": 1.0, "score": 345.0, "evalue": 2.83e-117, "gene_start": 1, "gene_end": 185, "reference_start": 1, "reference_end": 185, "reference_sequence": "MSFIHKLIQKFKPKKKLVDQVQQAVQEKSFFQANQKSYYQGLNKSANSFANTINKLAANYVTVNEQFQESLFEELVLLDIGYHAATKICDAIVQELKLQRVSDPQLIQEIIVDKLIVYYIQDKLFETDLTVEANKTNVYLFVGVNGVGKTTSLAKLADQLTKQNKRVLMVAGDTFRAGAVAQLAEWAQRIGCDIVLPNPKEETPAVIFRGVQQGIQNEYDFVLCDTSGRLQNKTNLMNELKKIYQIVQKVSSAKPQETLLVLDGTTGQSGLAQAKVFNEFTELTGIILTKMDSSSKGGIILAIKDLFNLPVKLIGFGETTADLAAFDLEQYVLGLTKNLSLNHEPNQT" }, "paralog": false, "identity": 0.9965034965034965, "score": 551.0, "evalue": 5.8e-201, "description": "Internal stop codon at 509190. Nonsense mutation around 509190.", "psc": { "uniref90_id": "UniRef90_P75362", "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P75362", "cog_id": "COG0552", "cog_category": "U", "kegg_orthology_id": "K03110", "go_ids": [ "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006614", "GO:0009306", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75362", "EC:3.6.5.4", "UniRef:UniRef50_P75362", "COG:COG0552", "COG:U", "KEGG:K03110", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006614", "GO:0009306", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75362", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75362" ], "product": "Signal recognition particle receptor FtsY" } }, "id": "GIHJHMOALN_924", "locus": "GCA009939745_00909" }, { "type": "cds", "sequence": "contig_1", "start": 509751, "stop": 512246, "strand": "-", "frame": 2, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261", "KEGG:K03529", "SO:0001217", "UniRef:UniRef50_P75361", "UniRef:UniRef90_P75361" ], "nt": "GTGGAAGCCAAACCAGAGGAGCGACGTAAGATCTTTGAGGACGCTTCGGGGATTGGGCGGTACACTAAGCGCAAAGAAGAGGTTACCAACCAATTAGCGCGTACCGTGCAAAATCTCAAACAGGTCAGCATTGTTTTAAATGAACTCAAAAAGGACTTAAAGAAGCTTACCATTCAAGCGGACAAGGCCCAGTGCTTTGTCAAACTTAAGGAAGAATTAAAGGAGCTGGAGCTGAGTGTCTTGGTCGCAGATTACCTCAAGTCCCAGGGTGAGTTAGACCGCTTTAACCACCAAATTGGTTACATCGAACAGGACTTTAAACTACACGAACCGCAGTTACAACTGCTAGAAGACCAGCTCAACATCTTTAACCAGCGCTTTCGTGATGCTGATGAACAGTCCATCAAACTACAACAAGAACTGCAAGCGGTCTACCAAACGATTAACGAGTTGGAACAACGCAAGGCCGTGATTGATGTGCAGCTCAAAAACGAGTTGTCCAAAAAGGACGAAAAGCACAAAATCCAGGCGTTAAAAAAGTTAATCCGGGTAGACCAAGCGCAACTAGAAAGCTTACAAGCGCAAGTACTCAAAACAACCAGTGAAATTACCCTGTTAACCAATGAGTTGAGCACCGTCCAAACTGAGTTGGACACCACAAAGTTGAATCTTAACCAAAACAGTGCGGCGTTAGTCTACCAACAAGCGCAGCAAGAGTTTTTAAAAGCGCAAAACGAGGAGTGGGTCAAAACGAATCCGGCCCATGTTTTGGTCAAAAATGTCAAGGCCCTCACCGGTCTCTTAAACACGCTCAACACTTTTTTAAAGTTTGAAAAGCAGTACGAAAAGGCCTTGTTAAAGGCGTTGGGTAAATCAATTGGTTATCTGGTTGTTAACAACAACTTGGCGGCTTTAAAGGCAATTGATTTTTTGTTAACTAACCAGATTGGTCAAGTTACCTTTTTACCAATTGACGACATTGCCTTTGATACTAAGATAGTTCCCGAGCACATGGAAATTTTGCAACAACTCGATGGCTTTTTGGGTGTTGGTAGTGACCATGTGAGTTGTGATGAAAGGTTACAACCAATTGTTAATGCTTTATTGGGTCAAGTAATTATTGCCAGTGACCTTCAAGCCGCTTTAAAACTGTCCAGTTATACCTATAAGCTGTACCGCGTTGTCACCCTCAACGGGGAAACGGTGTACGCTGGAGGGATTATCCAGGGTGGTTATGTCAAGGACAACTTAAGCTTGTATAACCTCCAGGAAAAGTTAGCTTCTAGTGAAGCTAACATTACCCAACTAGAACATAACGAAAAGCAACTACGTACCAATCTCACTAGCTTAGAGACTAAACTCAACGAGCTCAACAAAAAGTTAAAGTACGAAGAAATCCTACTGGAAAAGTTTAACGAACGTGTTAACCACACCAATAAGGCAATTTTGAGCTATAAGATTGAGTATGAGCAGCTCACCAACGAAAGCTTTGACGGAACCCCGCACAGCTTTGATGAAACCAGATTGGTTGAATCATTAAACCGTGCTTGGGCTGAACGTGACGAACTCAACTCACAGTTAAAGCTCAACCAGGAATTGAAAGAAACACTGGCCAAATCGATTAAACTAGCCGAAGCCAAAACCGCTGACCTCCGTGCCCTTTTAGATGAACAACGCTCCCAGTTAGTGTTGGCACGCGAAGGCAAAATCCGCTTTGAAAACACAATCCACAACATTACCGACAAGATTAACGGTGGGTATAAACTAACGATGGAGTTTGCGATCGCTAACTATAACAAACCAATTAAGCTCTCCACCATGCAAGCGCAAAACAAGATTGCCAGGATGCAGAGCCAACTCGATGAAATGGGTCCAATTAACCTGGAGTCGATTGCTGAAATTGCTGATAAGCAAAAGCGCTTTGATGACATTAACGGTGAGTATGAATCACTGCAAACGGCGATTAAGGATTTGCAAACCGCGATTGGTGAAATTGATGAATTGGCCTGCAAGGAATTTGACGAGCTCATCCAAAAAGTTAATGCAGAACTACCTAAAACCTTTAACTACCTCTTTGGCGGTGGTTCGTGTCAAATTCGCTACACGGATACTGATAACGTCTTGTTGTCAGGCATAGAAGTGTTTGCTAATCCGCCTGGCAAAAATGTCGCTAACTTAATGTTGTTATCGGGTGGGGAGAAAACACTCGTGGCCTTGTCGGTCCTCTTTAGTATTTTGAGAGTTAGTGCCTTTCCTCTAGTAATTTTAGATGAAGCGGAGAGTGCCTTAGATCCGGCGAACGTGGAGCGCTTTGCTAACATTATTGGCAATTCCAGTAACAACACCCAGTTCTTAATTATTACCCACCGTCAGGGCACGATGATGAAGTGTGACATGTTACTGGGAGCGGCAATGCAGACCAAGGGAGTGACCAAGACGTTTGCCGTTTCTTTGGAAAAAGCAGAGCAGTACATTAGTAAAGATAAGCAGAACTAA", "aa": "MEAKPEERRKIFEDASGIGRYTKRKEEVTNQLARTVQNLKQVSIVLNELKKDLKKLTIQADKAQCFVKLKEELKELELSVLVADYLKSQGELDRFNHQIGYIEQDFKLHEPQLQLLEDQLNIFNQRFRDADEQSIKLQQELQAVYQTINELEQRKAVIDVQLKNELSKKDEKHKIQALKKLIRVDQAQLESLQAQVLKTTSEITLLTNELSTVQTELDTTKLNLNQNSAALVYQQAQQEFLKAQNEEWVKTNPAHVLVKNVKALTGLLNTLNTFLKFEKQYEKALLKALGKSIGYLVVNNNLAALKAIDFLLTNQIGQVTFLPIDDIAFDTKIVPEHMEILQQLDGFLGVGSDHVSCDERLQPIVNALLGQVIIASDLQAALKLSSYTYKLYRVVTLNGETVYAGGIIQGGYVKDNLSLYNLQEKLASSEANITQLEHNEKQLRTNLTSLETKLNELNKKLKYEEILLEKFNERVNHTNKAILSYKIEYEQLTNESFDGTPHSFDETRLVESLNRAWAERDELNSQLKLNQELKETLAKSIKLAEAKTADLRALLDEQRSQLVLAREGKIRFENTIHNITDKINGGYKLTMEFAIANYNKPIKLSTMQAQNKIARMQSQLDEMGPINLESIAEIADKQKRFDDINGEYESLQTAIKDLQTAIGEIDELACKEFDELIQKVNAELPKTFNYLFGGGSCQIRYTDTDNVLLSGIEVFANPPGKNVANLMLLSGGEKTLVALSVLFSILRVSAFPLVILDEAESALDPANVERFANIIGNSSNNTQFLIITHRQGTMMKCDMLLGAAMQTKGVTKTFAVSLEKAEQYISKDKQN", "aa_hexdigest": "ad0984b63266a27acef35892758d9ba1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75361", "query_cov": 1.0, "subject_cov": 0.8462321792260692, "identity": 0.995, "score": 1431.0, "evalue": 0.0, "valid": true, "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_P75361", "cog_id": "COG1196", "cog_category": "D", "kegg_orthology_id": "K03529", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_P75361", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75361" ], "product": "Chromosome partition protein Smc" }, "genes": [ "smc" ], "id": "GIHJHMOALN_925", "locus": "GCA009939745_00910" }, { "type": "cds", "sequence": "contig_1", "start": 512250, "stop": 512699, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13175.12", "PFAM:PF13304.12", "PFAM:PF13476.12", "PFAM:PF13555.12", "PFAM:PF02463.25" ], "nt": "ATGGTTTTTCTCAAACGGTTTCGTGCGTATGGTTTTAAGTCATATGCTGACGAAATAACAATTAATTTTACCCACTCAATGACCGGTATAGTCGGTCCTAATGGTTCTGGCAAGTCCAACGTTGTCGATGCTTTGAAGTGGGTCTTAGGGGAACGCAGCATGAAGCACTTGCGCAGTAAGTCCGGTGATGATATGATCTTCTTTGGTTCTAAGGACAAGCCCGCTAGTAAGCTAGCGGAAGTGGAACTGACCTTTGACAATTCCCAAAAACTACTGCACGATCCCCGACCAGAGATCTCGGTGATGCGCCGGATCTATCGTGGTAGTGGTCAGAGTGAGTACTATATTAACGGCGAGTTAGTAACGCTAAAAGAAATTAGTGGGATCTTTGCTGATATTGGTTTGGAAAAAGGCTCGTTGGGGATTATTTCCCAGGGCAGTGTGTCGTGA", "aa": "MVFLKRFRAYGFKSYADEITINFTHSMTGIVGPNGSGKSNVVDALKWVLGERSMKHLRSKSGDDMIFFGSKDKPASKLAEVELTFDNSQKLLHDPRPEISVMRRIYRGSGQSEYYINGELVTLKEISGIFADIGLEKGSLGIISQGSVS", "aa_hexdigest": "a809eed79516bc538aaff6562854ba4f", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13175.12", "name": "AAA_15", "length": 65, "aa_cov": 0.4228187919463087, "hmm_cov": 0.7692307692307693, "evalue": 1.0279055379724189e-10, "score": 37.22410202026367, "start": 11, "stop": 73 }, { "id": "PF13304.12", "name": "AAA_21", "length": 67, "aa_cov": 0.3221476510067114, "hmm_cov": 1.0, "evalue": 1.996851048597718e-09, "score": 33.38786315917969, "start": 27, "stop": 74 }, { "id": "PF13476.12", "name": "AAA_23", "length": 124, "aa_cov": 0.8053691275167785, "hmm_cov": 0.9354838709677419, "evalue": 6.762178628412659e-18, "score": 61.50141525268555, "start": 11, "stop": 130 }, { "id": "PF13555.12", "name": "AAA_29", "length": 37, "aa_cov": 0.24161073825503357, "hmm_cov": 0.972972972972973, "evalue": 4.136921838432816e-08, "score": 28.39857292175293, "start": 14, "stop": 49 }, { "id": "PF02463.25", "name": "SMC_N", "length": 137, "aa_cov": 0.912751677852349, "hmm_cov": 0.9854014598540146, "evalue": 8.107914764587819e-43, "score": 141.84580993652344, "start": 3, "stop": 138 } ], "seq_stats": { "molecular_weight": 16498.679799999987, "isoelectric_point": 9.124742317199708 }, "id": "GIHJHMOALN_926", "locus": "GCA009939745_00911" }, { "type": "cds", "sequence": "contig_1", "start": 512884, "stop": 513756, "strand": "+", "frame": 1, "gene": "cof", "product": "Putative phosphatase MPN_427", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "RefSeq:WP_014325526.1", "SO:0001217", "UniParc:UPI0001DD63E3", "UniRef:UniRef100_A0AAX0S2J5", "UniRef:UniRef50_P75360", "UniRef:UniRef90_P75360" ], "nt": "ATGACAAAGACAAGTAAAGCATCCGGGTTAAGCTGGTTTTTTTGTGATTTAGATGGCACTTTACTGCGTTACCAAAACAACCAGCACTTAATTGAACCAACTACGAAGCGAGCAGTGGCACAGTTAGTGGAGAGTGGTGCCAACTTTGTTGTAGCTACTGGACGCAAACCGAGTGACGTGCGCAACATTTACAAGGAGTTGGGGATTGAACAAGCCAGTCCCTACTTAATTGCCAACAACGGCGCGGTAGTGTGGGATTTAAAGCGCAATAGCTACCTCAACAAGCAAACCTTGTCTTTAAGTGACTTTGACTTAATTGACCACATTAACCAAACGTTAAACCAATTAAACCACGAGTATGGCTGCATTCTGTATGGCCTCAACGACCAGGTGTACTTTTATCATATCCACGCTCCTGACTCACAGGCCTTCAAACAGTACTTTGCTTTTTATGAAGGCGAATTTGTCCAAAACCAGTATCTGGAAATAGATGGGCTCAAAACTGAATACAATCTAGTTAAAGCCATTTGGTTTTTTAAGGAAGTGCACCAGCAAAAGGCGGTGATTGCGCAACACTTTACTAACCAAGAACGCTTGGTCATTACTTCAGCACACTCGTTTGAACTAGTACCGCTTAATGTATCAAAGGGTCATGCGATAAACCTAATTAAACAACAAGTAAAAATCACCGATAACCAGATTATGGTCCTCGGTGACTCTTACAATGACTTGCCAATGTTTCAACATGGTGTAGTTAAGGTAACTAATCACCTCGCACCCGACAACCTCAAACAACTAGCTACAAGGGTTTATGACCTACCAGCTTCACTGTTTGTGGGTCAAGCGTTGAACGACTACTTTAAGTTCGATTAA", "aa": "MTKTSKASGLSWFFCDLDGTLLRYQNNQHLIEPTTKRAVAQLVESGANFVVATGRKPSDVRNIYKELGIEQASPYLIANNGAVVWDLKRNSYLNKQTLSLSDFDLIDHINQTLNQLNHEYGCILYGLNDQVYFYHIHAPDSQAFKQYFAFYEGEFVQNQYLEIDGLKTEYNLVKAIWFFKEVHQQKAVIAQHFTNQERLVITSAHSFELVPLNVSKGHAINLIKQQVKITDNQIMVLGDSYNDLPMFQHGVVKVTNHLAPDNLKQLATRVYDLPASLFVGQALNDYFKFD", "aa_hexdigest": "ea889d1df022db654c8c8fbf827c0aa8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63E3", "ncbi_nrp_id": "WP_014325526.1", "uniref100_id": "UniRef100_A0AAX0S2J5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63E3", "RefSeq:WP_014325526.1", "UniRef:UniRef100_A0AAX0S2J5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S2J5", "uniref90_id": "UniRef90_P75360" }, "psc": { "uniref90_id": "UniRef90_P75360", "gene": "Cof", "product": "Putative phosphatase MPN_427", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P75360", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P75360", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75360" ], "product": "Putative phosphatase MPN_427" }, "genes": [ "cof" ], "id": "GIHJHMOALN_927", "locus": "GCA009939745_00912" }, { "type": "cds", "sequence": "contig_1", "start": 513762, "stop": 514634, "strand": "-", "frame": 2, "gene": "pta", "product": "phosphate acetyltransferase", "db_xrefs": [ "COG:C", "COG:COG0280", "EC:2.3.1.8", "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959", "SO:0001217", "UniRef:UniRef50_P47541", "UniRef:UniRef90_P75359" ], "nt": "ATGGAAGCAGTTAACCAGCTCCAGCAAGCGGGTATTTTAACCCCAGCGGTGATCTTCCGCACCCGCAGTGAAGTACCAGCTGGTTTTAACACCGCCATCAAGCACTATGTGATTGAGGAAATGGACTTGACAAAGTACGCTAACTTCGTTTATGAAAAGCGCAAGCACAAGGGCATGGAAATGCGTGAAGCACAAAAGTTTGTCCGTGATGCCAGTTCCCTAGCAGCCACTTTAGTAGCACTCAACGAAGTGGATGGGGAAGTGTGTGGTAAGGAATACGCTACGAAGGATACTCTCCGTCCTGCTTTACAACTGTTAGGTACTGGTAACTTTGTGTCGAGTGTGTTCATCATGGAAAAGAATGAGGAACGCTTGTACTTTACCGACTGTGCTTTTGCTGTTTACCCTTCACCCCAAGAATTAGCAGTTGTAGCGGAAAACACCTTTAAGTTTGCCCAAAGTATGGGGGAACCAGAACTCAAGATGGTCTTTTTGAGCTATTCAACACTGGGCAGTGGTAAAGGGGAAGCGGTGGACAAAGTAGTCAGTGCGACCCAAATCTTCTTGGAAAAACACCCCGAACTCAAAGCTAATGTTTGTGGTGAATTACAGTTTGACAGTGCTTTTGTGGAAAAGGTACGCAAACAAAAAGCCCCTAATTTGACTTGGAATGGATCAGCTAACATCTATGTGTTCCCGAACCTAGATGCTGGTAACATTGGATACAAGATTGCGCAACGTTTAGGTGGTTATGAAGCAATTGGTCCAATTGTTTTAGGTCTAGCACGTCCTTTTAACGACCTTTCCCGTGGTGCCAGTGTCAGTGATGTCTTTAACGTGGGCATTATTACCGCAGCGCAAACACTTAAATAA", "aa": "MEAVNQLQQAGILTPAVIFRTRSEVPAGFNTAIKHYVIEEMDLTKYANFVYEKRKHKGMEMREAQKFVRDASSLAATLVALNEVDGEVCGKEYATKDTLRPALQLLGTGNFVSSVFIMEKNEERLYFTDCAFAVYPSPQELAVVAENTFKFAQSMGEPELKMVFLSYSTLGSGKGEAVDKVVSATQIFLEKHPELKANVCGELQFDSAFVEKVRKQKAPNLTWNGSANIYVFPNLDAGNIGYKIAQRLGGYEAIGPIVLGLARPFNDLSRGASVSDVFNVGIITAAQTLK", "aa_hexdigest": "151464f33bb12b05af6bc4ab739144ff", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75359", "query_cov": 1.0, "subject_cov": 0.90625, "identity": 0.997, "score": 570.0, "evalue": 9.08e-205, "valid": true, "gene": "pta", "product": "phosphate acetyltransferase", "ec_ids": [ "2.3.1.8" ], "uniref50_id": "UniRef50_P47541", "cog_id": "COG0280", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959" ] }, "pscc": { "uniref50_id": "UniRef50_P47541", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47541" ], "product": "Phosphate acetyltransferase" }, "genes": [ "pta" ], "id": "GIHJHMOALN_928", "locus": "GCA009939745_00913" }, { "type": "cds", "sequence": "contig_1", "start": 514762, "stop": 515076, "strand": "-", "frame": 1, "gene": null, "product": "phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021F8CEA1" ], "nt": "ATGTCCTTGGATGTGGGTAGCAAAACAGTGGCCCTGTTTAGCAGTTACCTCGCCAAGGCCAAAACAATCTTTTGGAATGGTCCCATGGGCGTATTTGAATTTAGCAACTATGCTCAGGGTACCTTAGCAATTGGTAAAGCGATTGCGCAAAACCAACAAGCCTTTAGTGTCATTGGCGGTGGTGATTCTGCAGCCGCTGCTAAACAACTCCAGATTGCTGATCAATTTAGTTTTATTTCCACTGGTGGTGGTGCATCTTTAGCACTAATAGGTGGGGAAGAGCTAGTGGGAATTAGCGATATTCAAAAAAAGTAA", "aa": "MSLDVGSKTVALFSSYLAKAKTIFWNGPMGVFEFSNYAQGTLAIGKAIAQNQQAFSVIGGGDSAAAAKQLQIADQFSFISTGGGASLALIGGEELVGISDIQKK", "aa_hexdigest": "583382ee057a7fee52f78e5db83ec487", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021F8CEA1", "query_cov": 0.8269230769230769, "subject_cov": 0.8958333333333334, "identity": 0.605, "score": 105.0, "evalue": 8.99e-28, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_UPI0021F8CEA1", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_UPI0021F8CEA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021F8CEA1" ], "product": "phosphoglycerate kinase" }, "genes": [], "id": "GIHJHMOALN_929", "locus": "GCA009939745_00914" }, { "type": "cds", "sequence": "contig_1", "start": 515098, "stop": 515544, "strand": "-", "frame": 1, "gene": null, "product": "phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000851E545" ], "nt": "TTGGGTGACATCTTTGTCAATGACGCCTTTGGCACCGCCCACCGCCGTCACGCTTCCAACGCTGGCATTGCCAAGTACATCAAAAACTCCTGCATTGGTCTTTTAATGGAGCGGGAATTGATCAACCTCTACCGCTTAATTAATAACCCACCCAAACCGTTTGTGGTTGTGTTGGGTGGCGCTAAGGTTTCTGATAAGTTACAGGTAGTAAATAACCTGCTCAAGATTGCGGATCATATTTTAATAGGTGGGGGCATGGTCAACACCTTCCTCAAAGCTCTGGGTAACGAAGTTGGCACTTCCTTAGTGGAGGAAGACTTAGTCCAAACCGCCAAGCAAATCCTTGACAGTGACAAGGACAAGAAAATTGTCTTGGGGGTAGACCAAATGCTTCATGCTAGCTTTAAGGATGAACCAGGCGTGGAGTGTGTTGTAGCCCCCCAATGA", "aa": "MGDIFVNDAFGTAHRRHASNAGIAKYIKNSCIGLLMERELINLYRLINNPPKPFVVVLGGAKVSDKLQVVNNLLKIADHILIGGGMVNTFLKALGNEVGTSLVEEDLVQTAKQILDSDKDKKIVLGVDQMLHASFKDEPGVECVVAPQ", "aa_hexdigest": "3d559ab8796abd2c7dd5ef7fa4b9b687", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00210B3D86", "query_cov": 0.9054054054054054, "subject_cov": 1.0, "identity": 0.5, "score": 134.0, "evalue": 1.17e-37, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_UPI000851E545", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_UPI000851E545", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000851E545" ], "product": "phosphoglycerate kinase" }, "genes": [], "id": "GIHJHMOALN_930", "locus": "GCA009939745_00915" }, { "type": "cds", "sequence": "contig_1", "start": 515551, "stop": 515991, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGGTTGACTTTAAAACAGTCCAAGCGTTTGACTTTCAGGGTAAAACCGTAGTCTTACGTGCTGACCTCAATGTGCCGATGAAGGACGGGGTCATTACCGACAACGAACGCATACTAGCTAGCTTAGACACGATTAAGTACCTCTTGGGTCACAATTGCAAAATTGTGTTACTGTCCCATTTGTCACGAGTCAAATCGCTCGATGACAAAAAGGGTAAGAAGTCACTCCAACCAGTAGCTAGTGCTTTACAAAATTTATTAAAGGACACCAAGGTTCACTTTTGTCCGGAAAACACGGGTGACAAGGTCAAAGTCGCAGTCAATCAACTCCCATTAGGGGAAATTTTGGTGTTGGAAAACACGCGTTACTGTGATGTGAATGAGGCTGGTGAAGTGGTTAAACACGAAAGCAAGAATAACGCTGAGTTAGCACAGTTCTGA", "aa": "MVDFKTVQAFDFQGKTVVLRADLNVPMKDGVITDNERILASLDTIKYLLGHNCKIVLLSHLSRVKSLDDKKGKKSLQPVASALQNLLKDTKVHFCPENTGDKVKVAVNQLPLGEILVLENTRYCDVNEAGEVVKHESKNNAELAQF", "aa_hexdigest": "5192ccd40eec708169cefd0dfc7f8ec9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 137, "aa_cov": 0.9315068493150684, "hmm_cov": 0.9051094890510949, "evalue": 1.3292808823011058e-36, "score": 121.81835174560547, "start": 8, "stop": 143 } ], "seq_stats": { "molecular_weight": 16230.581599999983, "isoelectric_point": 7.714608573913575 }, "id": "GIHJHMOALN_931", "locus": "GCA009939745_00916" }, { "type": "cds", "sequence": "contig_1", "start": 515994, "stop": 516716, "strand": "-", "frame": 2, "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "COG:COG0057", "COG:G", "EC:1.2.1.12", "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0016020", "GO:0016620", "GO:0031639", "GO:0034394", "GO:0050661", "GO:0051287", "SO:0001217", "UniRef:UniRef50_P47543", "UniRef:UniRef90_P75358" ], "nt": "GTGGTTGAATCCACTGGTCGCTTTGTTAGTGAAGAAGGTGCTAGTTTGCACTTGCAAGCTGGTGCTAAACGCGTGATTATTTCCGCTCCAGCTAAACAAAAAACAATTAAAACCGTGGTTTACAACGTGAACCACAAGATTATTAACGCTGAGGACAAAATTATCTCAGCTGCTAGCTGTACCACCAATTGCTTAGCACCAATGGTACACGTCTTGGAAAAGAACTTCGGCATCTTACACGGTACCATGGTAACGGTACACGCTTACACCGCCGACCAACGTTTACAAGACGCACCCCACAGCGATCTTCGTCGTGCCCGTGCTGCTGCTTGCAACATTGTACCTACCACCACTGGAGCTGCCAAAGCAATTGGCCTAGTAGTGCCAGAAGCTACTGGTAAGCTCAACGGTATGGCCCTTCGTGTTCCCGTACTAACTGGTTCGATCGTTGAACTTTGTGTAGCCCTTGAAAAGGATGCTACCGTAGAACAAATTAACCAAGCAATGAAGAAGGCGGCTTCCGCTTCCTTTAGGTACTGTGAGGATGAAATTGTGTCCAGTGACATTGTCGGCTCGGAACACGGTTCCATCTTTGACTCCAAGCTAACCAACATTATTGAAGTGGATGGTAACAAGCTTTACAAGGTTTACGCATGGTATGACAACGAATCCTCTTACGTAAACCAGTTGGTACGGGTAGTTAACTATTGTGCCAAGCTTTAA", "aa": "MVESTGRFVSEEGASLHLQAGAKRVIISAPAKQKTIKTVVYNVNHKIINAEDKIISAASCTTNCLAPMVHVLEKNFGILHGTMVTVHAYTADQRLQDAPHSDLRRARAAACNIVPTTTGAAKAIGLVVPEATGKLNGMALRVPVLTGSIVELCVALEKDATVEQINQAMKKAASASFRYCEDEIVSSDIVGSEHGSIFDSKLTNIIEVDGNKLYKVYAWYDNESSYVNQLVRVVNYCAKL", "aa_hexdigest": "49f813cf80852db2683f96dfcbf204c1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0012BB6B15", "query_cov": 0.9958333333333333, "subject_cov": 0.9409448818897638, "identity": 0.544, "score": 230.0, "evalue": 1.84e-72, "valid": false, "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A960BYG6", "go_ids": [ "GO:0006006", "GO:0016620", "GO:0050661", "GO:0051287" ] }, "genes": [ "gap" ], "truncated": "3-prime", "pseudogene": { "start": 515994, "stop": 517007, "observations": { "mutation": [ 516740 ], "stop": [ 516740 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75358", "query_cov": 1.0, "subject_cov": 0.712166172106825, "identity": 0.996, "score": 419.0, "evalue": 7.96e-146, "gene_start": 1, "gene_end": 240, "reference_start": 98, "reference_end": 337, "reference_sequence": "MLAKSKTIRVAINGFGRIGRLVFRALLSQKNIEIVAVNDLTHPDTLAHLLKYDSAHGEFKKKVVAKDNTLMIDKKKVLVFSEKDPANLPWAEHNIDIVVESTGRFVSEEGASLHLQAGAKRVIISAPAKQKTIKTVVYNVNHKIINAEDKIISAASCTTNCLAPMVHVLEKNFGILHGTMVTVHAYTADQRLQDAPHSDLRRARAAACNIVPTTTGAAKAIGLVVPEATGKLNGMALRVPVLTGSIVELCVALEKDATVEQINQAMKKAASASFRYCEDEIVSSDIVGSEHGSIFDSKLTNIIEVDGNKLYKVYAWYDNESSYVNQLVRVVNYCAKL" }, "paralog": false, "identity": 0.9970326409495549, "score": 608.0, "evalue": 6.78e-223, "description": "Internal stop codon at 516740. Nonsense mutation around 516740.", "psc": { "uniref90_id": "UniRef90_P75358", "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "ec_ids": [ "1.2.1.12" ], "uniref50_id": "UniRef50_P47543", "cog_id": "COG0057", "cog_category": "G", "go_ids": [ "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0016020", "GO:0016620", "GO:0031639", "GO:0034394", "GO:0050661", "GO:0051287" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75358", "EC:1.2.1.12", "UniRef:UniRef50_P47543", "COG:COG0057", "COG:G", "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0016020", "GO:0016620", "GO:0031639", "GO:0034394", "GO:0050661", "GO:0051287" ] }, "pscc": { "uniref50_id": "UniRef50_P47543", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47543" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" } }, "id": "GIHJHMOALN_932", "locus": "GCA009939745_00917" }, { "type": "cds", "sequence": "contig_1", "start": 516738, "stop": 517007, "strand": "-", "frame": 2, "gene": null, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X0XC69" ], "nt": "ATGCTAGCAAAGAGTAAGACTATCCGTGTTGCTATCAATGGATTTGGACGTATTGGACGCCTTGTTTTTCGTGCACTTTTAAGCCAAAAAAATATTGAGATCGTTGCCGTTAACGACCTCACCCATCCCGATACTTTAGCACACTTACTAAAGTACGACTCCGCTCACGGTGAGTTTAAGAAAAAGGTAGTAGCTAAGGACAACACCTTAATGATTGACAAAAAGAAGGTGTTAGTGTTTAGTGAAAAAGATCCTGCTAACCTGCCATGA", "aa": "MLAKSKTIRVAINGFGRIGRLVFRALLSQKNIEIVAVNDLTHPDTLAHLLKYDSAHGEFKKKVVAKDNTLMIDKKKVLVFSEKDPANLP", "aa_hexdigest": "c1be61464e39cd0954b4529b83755861", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X0XC69", "query_cov": 0.9213483146067416, "subject_cov": 0.9425287356321839, "identity": 0.537, "score": 92.8, "evalue": 5.82e-23, "valid": false, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A2X0XC69" }, "pscc": { "uniref50_id": "UniRef50_A0A2X0XC69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X0XC69" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [], "id": "GIHJHMOALN_933", "locus": "GCA009939745_00918" }, { "type": "cds", "sequence": "contig_1", "start": 517167, "stop": 517739, "strand": "-", "frame": 2, "gene": "ecfT", "product": "putative MG302-like protein", "db_xrefs": [ "COG:COG0619", "COG:H", "GO:0005886", "KEGG:K16785", "SO:0001217", "UniRef:UniRef50_P47544", "UniRef:UniRef90_P75357" ], "nt": "TTGGCCAGTCAAAATGAAAATTTTTTAGGTCATTTTATTTATTCAGGCGGTGAACAGTTTGGTGGGGTGAGTTGGTGGAGTGTCAATACCCGCTCTTTACTACGTTCATTGGTAATAGCACTACGAATTAGCATGCTGTTTGCCACGTCCTTTCTCTTAACTGCGTCCACTTCGATTTATGAGTTAGCGTTTGGGGTCGAACGGTTGTGTACCCCTTTGCGCTACCTCAAAATTAAAACACAACCGTTAAGCATCTTATTTGCACTGGTGTTTAAACTGTTACCCATTGTTAAGGGTGAATTAAAACGGATTAAACAAGCGCAAGCAATCCGTGGTTTTAAGTACGGTAAGCTAGCCTTTTTAAATCCAGTCAAACTCAAAACGCTCTTTATTCCGGTGTTATTGTCAACGGTCAAAAAAACCGAGGCAGTCGCGTTTGCGCTACAAGCCAAGGGTTACCAGTTGGACAATCCTAATAAAACGCACTATCTCCAAAAGTACAACCTTTGGGGTGGGATAGTCTTTCTGGGGCTTTTTGTCTTACTTAGTTGCTTATTAATGGTGAACAACTGA", "aa": "MASQNENFLGHFIYSGGEQFGGVSWWSVNTRSLLRSLVIALRISMLFATSFLLTASTSIYELAFGVERLCTPLRYLKIKTQPLSILFALVFKLLPIVKGELKRIKQAQAIRGFKYGKLAFLNPVKLKTLFIPVLLSTVKKTEAVAFALQAKGYQLDNPNKTHYLQKYNLWGGIVFLGLFVLLSCLLMVNN", "aa_hexdigest": "a75fbd3f2fd7e37bc8f9df0b6be3664d", "start_type": "TTG", "rbs_motif": null, "genes": [ "ecfT" ], "truncated": "both", "pseudogene": { "start": 517038, "stop": 517991, "observations": { "mutation": [ 517154, 517169, 517808, 517937 ], "stop": [ 517154, 517169, 517808, 517937 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75357", "query_cov": 1.0, "subject_cov": 0.5993690851735016, "identity": 0.995, "score": 365.0, "evalue": 7.46e-126, "gene_start": 1, "gene_end": 190, "reference_start": 85, "reference_end": 274, "reference_sequence": "MTSKNFLSQLPPVLKLWWWIISLVVAFLPLGLHGLIIINAIFFALVIVVERKLKTFAIIFGWLLFFFWFNIVVNGFIFLPNSSALASQNENFLGHFIYSGGEQFGGVSWWSVNTRSLLRSLVIALRISMLFATSFLLTASTSIYELAFGVERLCTPLRYLKIKTQPLSILFALVFKLLPIVKGELKRIKQAQAIRGFKYGKLAFLNPVKLKTLFIPVLLSTVKKTEAVAFALQAKGYQLDNPNKTHYLQKYNLWGGIVFLGLFVLLSCLLMVNNWHLVYWTNPHYSFTFTHQNFCFFKQISSPQLLAFWQLELLAIG" }, "paralog": false, "identity": 0.9873817034700315, "score": 616.0, "evalue": 5.89e-227, "description": "Internal stop codon at 517154, 517169, 517808, 517937. Nonsense mutation around 517154, 517169, 517808, 517937.", "psc": { "uniref90_id": "UniRef90_P75357", "gene": "EcfT", "product": "Uncharacterized protein MG302 homolog", "uniref50_id": "UniRef50_P47544", "cog_id": "COG0619", "cog_category": "H", "kegg_orthology_id": "K16785", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75357", "UniRef:UniRef50_P47544", "COG:COG0619", "COG:H", "KEGG:K16785", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47544", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47544" ], "product": "Uncharacterized protein MG302" } }, "id": "GIHJHMOALN_934", "locus": "GCA009939745_00919" }, { "type": "cds", "sequence": "contig_1", "start": 517981, "stop": 518910, "strand": "-", "frame": 1, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding MG303-like protein", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P75356", "UniRef:UniRef90_P75356" ], "nt": "GTGCACCCTTTTTATTATTTAACAGCTAAAAAACACACCCCGCTGTTTAACCAGCAGTTAGTTGATTTAGCGCAAAGTACGCTGTATTTTTACAACCTTAGTGTCTTTGTTGATAAAAGCAATGCTGGTCAAATTATTAAAAACGTTACCGGTAGTGTGGAACCTAACCAAATTACCGTTATTTTTGGTCCTAGTGGCAGTGGCAAAACGACCCTAATTAAACAGTTAGGTTTAGTTGAAAATCCGACCTGTGGTTTTTTGAACTGTGGTAACTTTTATTACTTTGCCAACCAAAAACATAACCGTGCCACAAAGCAGTTCCAAAACAGCATTGGCTATGTGTTACAAAAAGCCGAAGAACAGTTTTTCTGTGATTCCGTTTTAGAAGAGGTGTTAACGGGAGCGATTAACCTCGGTTTGTGTCAAAAGGGTGATGTCAACTTTGCCAAAAAGTACTTAGAGATGTGTGGTTTGGACCACATTCCCCTAATTAAAAACCCCTTGGAGTTGAGTGGCGGACAAAAGAAACGCTTGGCATTGGCGAGTGTATTAGCCATGCAAGTGCAATTTCTCATTTTGGACGAACCAACGGTCGGGTTAGACCAGGAGGGTAAAGCACTTAAAAGTGCTTTACTCAAACAATTAAAACAGGTCACGCGCATTATGATTGTCAGTCACGACGTTGATTTCATTTACGAAACCGCTGACAGTCTGATTCAATTAGAGGCTGGTCAAATTGTCGACCAAATGAGTGTTGCAGATTTCTTTAACAACATGCAGCTTTTACAACGGTACGAAATTACCCCACCACTTGTGGTGCAAACCATTCAGTTGTTACAAGCAAAGGGTGTGCAGTTAAACGATCCCTTAGCCATTAAAACCGTACACGATCTGATTGATCAACTCAAACCGCTCTTCCATGACCAGTAA", "aa": "MHPFYYLTAKKHTPLFNQQLVDLAQSTLYFYNLSVFVDKSNAGQIIKNVTGSVEPNQITVIFGPSGSGKTTLIKQLGLVENPTCGFLNCGNFYYFANQKHNRATKQFQNSIGYVLQKAEEQFFCDSVLEEVLTGAINLGLCQKGDVNFAKKYLEMCGLDHIPLIKNPLELSGGQKKRLALASVLAMQVQFLILDEPTVGLDQEGKALKSALLKQLKQVTRIMIVSHDVDFIYETADSLIQLEAGQIVDQMSVADFFNNMQLLQRYEITPPLVVQTIQLLQAKGVQLNDPLAIKTVHDLIDQLKPLFHDQ", "aa_hexdigest": "6fda083b9336e7474d6d4b2bc34b984a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75356", "query_cov": 1.0, "subject_cov": 0.8089005235602095, "identity": 0.997, "score": 611.0, "evalue": 9.55e-220, "valid": true, "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG303 homolog", "uniref50_id": "UniRef50_P75356", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P75356", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75356" ], "product": "Putative ABC transporter ATP-binding protein MG303 homolog" }, "genes": [ "ecfA2" ], "id": "GIHJHMOALN_935", "locus": "GCA009939745_00920" }, { "type": "cds", "sequence": "contig_1", "start": 519126, "stop": 519836, "strand": "-", "frame": 2, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding MG304-like protein", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47546", "UniRef:UniRef90_P75355" ], "nt": "TTGCTCAACGTTACCAACCTCAGTTTTACCTATCCGCGCCACAGTCAACCGGCCTTAGAAAAGCTCAATTTTAGCCTCAAACCCAACACCCATGTAGCCATTATTGGCCACAATGGTTCGGGTAAATCGACACTGGTCAAACTGTTAGGCGGATTTTTAAAAGCACCGAAACAAACAATCTTTTTTAGGGGTCAACCCTTGGAAGAAGTTGGTTTTCGCCAAATTGGTATCTTACTGCAAGATCCCGACATGCAACTGTTGGGTGATACGCTGCACCAAGAGCTCATTTTTAGCTTGGAAAACCACGGGGTGGATCCTAAACAAATGGACCGAGTGATTGCTGATGTTTTAGCGGTTGTAGAACTACAAGGTAAACAGTTCACACCGCTCAGTAAGCTGTCGTTTGGCGAAAAACAACGGGCCGTGTTTGCTTGTTTACTGGCGGTGAAACCCGAACTTTATTTACTAGATGAAGCCTTTAGTATGTTGGATGGTAAGCTAGCTAGCAAACTAAAGCGGTTCATTTTTAAGGTAATTAAAGAACAGCAAAAAACAGTTATTAATGTTACCCATGACTTTAATGACCTCTTCTTAGCGGATGAAATTATCTTTTTAAGTAAGGGACAGCTGTTAAAACAGTTTTCACCCGCTGCCATATACAAACAGCTCCACTTGTTCCACCAGCACCACTTTACCCTCCCGTTTCCATGA", "aa": "MLNVTNLSFTYPRHSQPALEKLNFSLKPNTHVAIIGHNGSGKSTLVKLLGGFLKAPKQTIFFRGQPLEEVGFRQIGILLQDPDMQLLGDTLHQELIFSLENHGVDPKQMDRVIADVLAVVELQGKQFTPLSKLSFGEKQRAVFACLLAVKPELYLLDEAFSMLDGKLASKLKRFIFKVIKEQQKTVINVTHDFNDLFLADEIIFLSKGQLLKQFSPAAIYKQLHLFHQHHFTLPFP", "aa_hexdigest": "d6dc03ce116bce752013486a5f58541f", "start_type": "TTG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_P75355", "query_cov": 1.0, "subject_cov": 0.8676470588235294, "identity": 0.996, "score": 466.0, "evalue": 2.48e-165, "valid": true, "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG304 homolog", "uniref50_id": "UniRef50_P47546", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P47546", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47546" ], "product": "Putative ABC transporter ATP-binding protein MG304" }, "genes": [ "ecfA2" ], "id": "GIHJHMOALN_936", "locus": "GCA009939745_00921" }, { "type": "cds", "sequence": "contig_1", "start": 520054, "stop": 521781, "strand": "-", "frame": 1, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_P9WMJ9", "UniRef:UniRef90_P47547" ], "nt": "ATGAGTACAGATAACGGCTTAATTATCGGCATTGACCTTGGTACCACTAACTCCTGTGTGTCGGTCATGGAGAATGGACGCCCAGTAGTGTTGGAAAACCCTGAAGGTAAACGCACCACCCCTTCGATTGTTTCTTACAAGAACAACGAAATTATTGTGGGTGATGCTGCGAAACGGCAAATGGTAACTAACCCTAATACTATTGTTTCCATTAAGCGTTTAATGGGTACCTCCAATAAGGTAACCGTTAAGAATCCTGATGGTTCTACCAAAGAGTTAACTCCTGAAGAGGTATCAGCGCAAATCTTGAGCTACCTCAAGGACTATGCGGAAAAGAAGATTGGTAAAACGATTTCCCGTGCTGTTATTACCGTACCTGCTTACTTTAACGATGCAGAACGGAACGCTACTAAAACCGCTGGTAAGATTGCTGGTTTAAACGTTGAGCGGATTATTAACGAACCTACCGCCGCTGCATTGGCTTATGGGATCGACAAGTCTAACCGAGAAATGAAAGTCTTGGTGTACGACCTTGGTGGTGGTACCTTTGACGTTTCCTTACTTGACATTGCTGAAGGTACCTTCGAAGTATTAGCCACTGCTGGGGACAACCGTTTGGGTGGTGATGACTGGGACAACAAGATTATTGAGTTCATCTTAGCGCACATTGCCCAAGAACACAATGGGCTTAACTTGTCCAATGACAAGATGGCTATGCAACGCTTAAAGGAAGCGGCTGAACGTGCTAAGATTGAACTTTCCGCCCAACTAGAAGCAATTATCTCTTTACCGTTCTTAACGGTTACCGAAAAGGGTCCGGTAAACGTTGAACTTAAGCTAACCCGTGCTAAGTTTGAAGAAATTACCAAACAATTACTAGAACGTACTCGCAACCCAATTTCGGATGTTTTACGTGAAGCCAAGATTAAACCAGAAGAAATTAATGAAATCTTGTTGGTGGGTGGATCGACCCGGATGCCAGCAGTGCAAAAACTAGTGGAATCAATGGTACCAGGACACAGTCCAAACCGCTCAATTAACCCGGATGAGGTGGTAGCCATTGGTGCTGCCATCCAAGGGGGTGTGTTACGCGGTGATGTAAAGGACGTGTTACTGTTGGACGTTACTCCTTTAACGCTCTCGATTGAAACCCTTGGTGGTGTAGCAACTCCGTTAATTAAGCGTAACACCACCATTCCTGTAAGTAAGAGTCAAATCTTCTCTACAGCGCAAGACAACCAAGAATCAGTGGATGTGGTGGTTTGTCAAGGGGAACGCCCAATGGCACGTGACAACAAGTCTTTGGGTCGCTTTAACTTAGGGGGCATCCAACCAGCACCCAAGGGTAAACCCCAAATTGAAATTACCTTTAGCTTGGACGCCAACGGGATCTTAAACGTGAAGGCTAAAGATTTAACCACTCAAAAGGAAAACAGTATTACTATTAGTGACAACGGCAACTTGTCCGAAGAGGAAATCCAAAAGATGATTCGTGATGCGGAAGCCAACAAGGAGCGTGACAATGTGATTCGTGAACGCATTGAGCTCCGTAACGAAGGTGAAAGCATCGTGAGCACGATTAAGGAGATTCTCCAAAGTCCCGAAGCGAAGGACTTCCCTAAAGAAGAGAAGGAAAAACTCGACAAGATTACCGGTGGTATTGATGCAGCAATTAAGGCCAATGACTACACCAAGTTAAAAGCCGAAATCGAAAACTTCAAGAAGTGA", "aa": "MSTDNGLIIGIDLGTTNSCVSVMENGRPVVLENPEGKRTTPSIVSYKNNEIIVGDAAKRQMVTNPNTIVSIKRLMGTSNKVTVKNPDGSTKELTPEEVSAQILSYLKDYAEKKIGKTISRAVITVPAYFNDAERNATKTAGKIAGLNVERIINEPTAAALAYGIDKSNREMKVLVYDLGGGTFDVSLLDIAEGTFEVLATAGDNRLGGDDWDNKIIEFILAHIAQEHNGLNLSNDKMAMQRLKEAAERAKIELSAQLEAIISLPFLTVTEKGPVNVELKLTRAKFEEITKQLLERTRNPISDVLREAKIKPEEINEILLVGGSTRMPAVQKLVESMVPGHSPNRSINPDEVVAIGAAIQGGVLRGDVKDVLLLDVTPLTLSIETLGGVATPLIKRNTTIPVSKSQIFSTAQDNQESVDVVVCQGERPMARDNKSLGRFNLGGIQPAPKGKPQIEITFSLDANGILNVKAKDLTTQKENSITISDNGNLSEEEIQKMIRDAEANKERDNVIRERIELRNEGESIVSTIKEILQSPEAKDFPKEEKEKLDKITGGIDAAIKANDYTKLKAEIENFKK", "aa_hexdigest": "c4c9d98b7612066c9a72eb32f77d0427", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47547", "query_cov": 1.0, "subject_cov": 0.9663865546218487, "identity": 0.934, "score": 1022.0, "evalue": 0.0, "valid": true, "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_P9WMJ9", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_P9WMJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P9WMJ9" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "GIHJHMOALN_937", "locus": "GCA009939745_00922" }, { "type": "cds", "sequence": "contig_1", "start": 521996, "stop": 522313, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGGTTTAGAAATTAAACGTTTAGGCTTGACGATCTTCAAATTACTAGCCAATATCTTAATAGCAGTGGTGGGTTACTTTAGTGTGTTTGCTGTTTTTGCCATTGTCAAAAAGGAAAACGCAACTAACAACGTTATTGACCAGTACTCTACGGCTGACATTTTCAACATCACTAACATTGCCATTACCGCTTCTAACTTTATTCCCGTACCTAGTGGGGAAGGTGCTACCCAGTTTGTAATGACAAGCTTTTTAAATGCCTTTAAATCGGCTGTTGGTATTGAATCGCAAGTAAAGCAAGGGGTCTTTTTGTGA", "aa": "MYGLEIKRLGLTIFKLLANILIAVVGYFSVFAVFAIVKKENATNNVIDQYSTADIFNITNIAITASNFIPVPSGEGATQFVMTSFLNAFKSAVGIESQVKQGVFL", "aa_hexdigest": "706b7193aaa74d2f01bbae0e318addc0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11385.160599999992, "isoelectric_point": 8.00776309967041 }, "id": "GIHJHMOALN_938", "locus": "GCA009939745_00923" }, { "type": "cds", "sequence": "contig_1", "start": 522383, "stop": 522484, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTGATGTGGTGGTAGCCATCTTATTTATCTTTATTGCCTACAGTAAAAGGATGCATGTCTTAATTTACTCCGGGGTAAACCAGTTTCGTAAGTGA", "aa": "MIFDVVVAILFIFIAYSKRMHVLIYSGVNQFRK", "aa_hexdigest": "38a2d1f03e38d08d6a172cce833d66d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3919.7433, "isoelectric_point": 9.992035484313966 }, "id": "GIHJHMOALN_939", "locus": "GCA009939745_00924" }, { "type": "cds", "sequence": "contig_1", "start": 522572, "stop": 522727, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGTTCTTTCAAATTGTCACCCCACTTTCCTTGGGTCAAGATCCCTTTCGCTTGTACTGGTTCATTAAAAAGGGCATGAAGAAGCAGACAGCGGTCTTATTAGTTACTTCCACGGGCGCCTTTTGGAACCTTGCCCAAGCGTTAATTACTTGA", "aa": "MQFFQIVTPLSLGQDPFRLYWFIKKGMKKQTAVLLVTSTGAFWNLAQALIT", "aa_hexdigest": "a1c0539050506c6ffe39ed05945a04fb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5848.919200000001, "isoelectric_point": 10.171064186096192 }, "id": "GIHJHMOALN_940", "locus": "GCA009939745_00925" }, { "type": "cds", "sequence": "contig_1", "start": 522857, "stop": 523024, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAACCCCTAAAAGCGCTAGTTTTTTTACTAGAAAAAATATTCTTGCCTTTAGCTTTTTTATTGCCTTTTTGGTGGTTGTTAGTGTATTGGTGACCGTCTTCTTTTTAGACATTAAAACTGGTGATGTTAAAACGATTATTAACACCATTAACAGAACTAACTGA", "aa": "MSTPKSASFFTRKNILAFSFFIAFLVVVSVLVTVFFLDIKTGDVKTIINTINRTN", "aa_hexdigest": "e0141c928abccabe149aedb1b9e4099d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6174.254499999999, "isoelectric_point": 10.446408653259276 }, "id": "GIHJHMOALN_941", "locus": "GCA009939745_00926" }, { "type": "cds", "sequence": "contig_1", "start": 523034, "stop": 524488, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_437", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75341" ], "nt": "TTGGAAAACATCCAAACTAAAGTAAATGATTTTGTTAAAAACAAACTCAAACCTGCTTTAGCACCCCGACCAGCTTACAGTAACGTCATTTTGTTAAACGTTAACAATGACAAAGTACTTTCTAGTGGTGCTAACTGGAGCTTAGCTAGCTTATTACAGAGCGACAAAGTCAACCCGCTTTCGTTTATGTTGCTCAAACAAGCGTTCGATAATAACGATTTATTTAAGAAAGCACAAAAGTTGTTTAAAGACATTCAGGAAAAGTCCAGTAATAATGGTGGAATGCAAAGTAGTTCCACAACCAACAGTGATGCCGATGCATTAAGTAAAGTTATCGGTAACTATTACTACACTACTTGGGCTAAACTCACGGACAAATCGATTTACGGTAACCCGAAGGACAACAAGTTTGATGAGCTTTTCAAACTGGCTTTTGAAGCTAGCATCGACGAAAAGTCCTTTAACGTTGATTACAAAGCGGTCATTGACCATTACCGTTTTATCTATACCTTACAGTGGCTAGTGGACCAAAAATTAAAGAACTTTAAGTCACTGTTAAAAACAAACCTTAAGTTTGGTGAAGTTGCTTTTATAGCTTACAAGAACACTGAAACCACTAACTTCTCTAATCCCCAAGGCGTATTTGGTTCCTACTTCAACTATGAGAACTCCGCTAACGAAGTTAAAGAATCCACTCAAACACTAGATCCCAATAACTTCTTTTACAAAACAACAACCAAGCCAACGGTACAAGCCACTCAACAAGTTGCTAGCTTAGCATTAGTACAAAAACAACAAATGCAACAAAATTCAACTGATCACTATGGTTTTACTGGTTTGAGTACCAGCACTAGTTCGATGTTCGATGCTAGTTCCCGTGATGCCATTTTGCAGCAAATTACGAAAACCTCCTTGCAACAGTACGGTTCCAAGGATCAACTCAAAAAGATCATTCAAGGGACTAATAACCAATTGTTACTAGACCGGATTGCAGTCCAGTTAAGTGGATTAAATCCTTCCACAACTAATGGTGGCAGTGGCAAAACAATTGCGACCTACTTCCAAGTGGATGCCGTTGGCAACCCAACTTTGGACTTCCAAGCTAAGCGTCAACTGTTATTAGACCTCTTGGACCAGTACCAAAACTACTTTGGTAATGGTGCCCAAAAGAGCCAAAGGGATTCTACTCCAAGTGGAACTGGCAACTACCTCACTTACCAAAATGGTAGTGACAAGTACACCTACACCCAGTTCACTTACCAAGACATTGACAGCTTGAGTCTAACAACTACAAGCGGTACCAACAATAAAATTGCCAGTGATGTTGTGGCAGCGCTGCTTTTATTCCAAGCAGCAGACAAGGGCACACAACAGTTGGCACTTAGTGCCATTAATAAGCCGCAGTTAAATATTGGTGATAAACGGATAGAAAGTGGGTTAAAATTGCTCAAATAG", "aa": "MENIQTKVNDFVKNKLKPALAPRPAYSNVILLNVNNDKVLSSGANWSLASLLQSDKVNPLSFMLLKQAFDNNDLFKKAQKLFKDIQEKSSNNGGMQSSSTTNSDADALSKVIGNYYYTTWAKLTDKSIYGNPKDNKFDELFKLAFEASIDEKSFNVDYKAVIDHYRFIYTLQWLVDQKLKNFKSLLKTNLKFGEVAFIAYKNTETTNFSNPQGVFGSYFNYENSANEVKESTQTLDPNNFFYKTTTKPTVQATQQVASLALVQKQQMQQNSTDHYGFTGLSTSTSSMFDASSRDAILQQITKTSLQQYGSKDQLKKIIQGTNNQLLLDRIAVQLSGLNPSTTNGGSGKTIATYFQVDAVGNPTLDFQAKRQLLLDLLDQYQNYFGNGAQKSQRDSTPSGTGNYLTYQNGSDKYTYTQFTYQDIDSLSLTTTSGTNNKIASDVVAALLLFQAADKGTQQLALSAINKPQLNIGDKRIESGLKLLK", "aa_hexdigest": "919334446a07c5d82f1198222e4619ab", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75341", "query_cov": 0.9772727272727273, "subject_cov": 0.8269230769230769, "identity": 0.7190000000000001, "score": 634.0, "evalue": 9.21e-223, "valid": false, "product": "Uncharacterized protein MPN_437", "uniref50_id": "UniRef50_P75341" }, "pscc": { "uniref50_id": "UniRef50_P75341", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75341" ], "product": "Uncharacterized protein MPN_437" }, "genes": [], "id": "GIHJHMOALN_942", "locus": "GCA009939745_00927" }, { "type": "cds", "sequence": "contig_1", "start": 524582, "stop": 525670, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGCAAATTAAGGAGCTCAAAGAAGACCTTAACAACACGATTGTTGTCGATAAAAGTAAGGACGTAGAAAAAGCTTCCAAAACTAACGCACTGTTTTACAGTGATCAAGAAGGTAAGCAACAACAAAGTGACTCAGATCCAATTGCTGGCGCTTTAGATGACATTTTTGCTCAAAACACAAGCGAAGGCACTAACTTAAGTAAGTTAGCGGAGCAGGTAAAGAAAGCAGCTGCAACAAAAATGGAAGCGAAAACAGCTGTTTTAAGAACTAACAATTCTAAGGGCCAACAAAACAATTACGTTGTTTTAGATGCAGCTATTCCTACATTTAATTCAACAACATCAAAATCGAAAAATAATAGCGCTTCTAATGAAGTTTTAGTTGCCTTAAAATCTGGATCTATAAATTTAAGGCAGGTTCAGCAAACTGATCAAAACAGTTACAGTCCCATTAAATTCCGGATTGTGCGTAATAGCACCGGAGTAACTGTTTTCGGCCTTGATGGTGGGAGCTATTATTTAAAACAAGATTCAACAAATAAAAAATCAGTTTCTAAGCAAAGTTTAACCTTATTAACTAAATCTAGTTCAGGTAACAGTAATAAAGTATTAAGAGACCTTGACAAGCAAAAACAATTCTTAAAGTTTCGTGCCTTTCAAGCTAAAACTAACACTTTCTACAGTACTAACTTTGCCTTTAGTTTTCCCTTAAACGAAACGCTAAAAAGTTGGTTTGATAAACACCGGGAACTAATTTTGGCCAACGCCTTAGTTAACGCTAGTCTTGACCAAAAGGATAAAGCTAGCAAAGCTTTAACTGAAGCTTTTAATCCTTATAAAGAGTTAATTAAAGAATTTGCGCCGGTGGCTTTAGCAACCACAATGATCAGCTTTTATTTTGATCAAATGAAAGCGCTCAATAACAAGTTGCTAGAGCGCGCCCGTAATCTTAACCAAAACGTCAACCAAGCCAATCCAACCCCTTGGTTGAACGGTTTGTCAGCCAAGTTACCTTACGTTAATACTAACGGTAATTACGAAAAGTTAAACAACTACTTTACCTTCTTAATCACAAAAACATTGTGA", "aa": "MPQIKELKEDLNNTIVVDKSKDVEKASKTNALFYSDQEGKQQQSDSDPIAGALDDIFAQNTSEGTNLSKLAEQVKKAAATKMEAKTAVLRTNNSKGQQNNYVVLDAAIPTFNSTTSKSKNNSASNEVLVALKSGSINLRQVQQTDQNSYSPIKFRIVRNSTGVTVFGLDGGSYYLKQDSTNKKSVSKQSLTLLTKSSSGNSNKVLRDLDKQKQFLKFRAFQAKTNTFYSTNFAFSFPLNETLKSWFDKHRELILANALVNASLDQKDKASKALTEAFNPYKELIKEFAPVALATTMISFYFDQMKALNNKLLERARNLNQNVNQANPTPWLNGLSAKLPYVNTNGNYEKLNNYFTFLITKTL", "aa_hexdigest": "4fba2028c855791ba036ed9cbad188d9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 40403.07009999999, "isoelectric_point": 9.588463401794431 }, "id": "GIHJHMOALN_943", "locus": "GCA009939745_00928" }, { "type": "cds", "sequence": "contig_1", "start": 525932, "stop": 526117, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGTACACTGATCCCACTTTAATTAGTCAAGTTAACTATAAGTATTCTGCTCCCAGTCAAGGGTTAGGTCAGATCTATAACAGAGAGAAGTTAAAGGATAAACTAACACCTTCTTATGCCTTTCCTTTCTTTGCCGAAGAAAAAGACATTGCACCCAACCAAAACGTTGGTAATAAGCGCTGA", "aa": "MEYTDPTLISQVNYKYSAPSQGLGQIYNREKLKDKLTPSYAFPFFAEEKDIAPNQNVGNKR", "aa_hexdigest": "cf66cb80295171112421c4c0f42a0c3f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7015.779500000002, "isoelectric_point": 7.961990547180176 }, "id": "GIHJHMOALN_944", "locus": "GCA009939745_00929" }, { "type": "cds", "sequence": "contig_1", "start": 526205, "stop": 526570, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_439", "db_xrefs": [ "GO:0098552", "SO:0001217", "UniRef:UniRef50_P75339", "UniRef:UniRef90_P75339" ], "nt": "GTGGCCAACAAACAATTTATCGCTGCACCACTTTTAAAAGCACTAGAAGCTTGGTACGAAAACAACGAAGACAAAAAGATTACCCAGTTTTTAAAAGACACTAAGTCCAATGTTGACAGTCAGTACACAACGGCAGTCGATAAAGTGGTATCAGCATCACGCAATAAATCACTTTTTGTGCAACAAGATTTGTTGGATAACGCCGGTGGTAGTGAAGCAACCTGGAAAGCGCAAAAGCTGCTTGAACAGCTCATTAGTGACTTTGCTAGTCGGGTTTTCCAAAAGAACTACCTCAATTACAAAAAAGATGGACAAGTTTCTACTGGTCCATTTACTTATGATGAACTACACAAGGAAGAAAGCTGA", "aa": "MANKQFIAAPLLKALEAWYENNEDKKITQFLKDTKSNVDSQYTTAVDKVVSASRNKSLFVQQDLLDNAGGSEATWKAQKLLEQLISDFASRVFQKNYLNYKKDGQVSTGPFTYDELHKEES", "aa_hexdigest": "f6b67903329ec1df8755fa34cab58ee5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 525942, "stop": 526768, "observations": { "deletion": [ 525982 ], "mutation": [ 526207, 526738 ], "stop": [ 526207, 526738 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75339", "query_cov": 0.9586776859504132, "subject_cov": 0.4218181818181818, "identity": 0.81, "score": 192.0, "evalue": 1.8e-59, "gene_start": 3, "gene_end": 118, "reference_start": 69, "reference_end": 184, "reference_sequence": "MKSFLRKPKFWLLLLGGLSTSSIILSACATPSNSALQAVFKPTSNQFFNGEHGTIQSALNTALRDPETNKKFVAAPLLKALEAWYENNQDKNITQFLKDTKTNVDNQYKTVVDKVVSAPRNKSLFVQQDLLDSSGGSEATWKARKLFEQLISDFASRVFQKNYLSYKENGKVSAGPFLYDTISKNSNWQNIVFDAGNFPETNDDFFAQIQSEVFDQWAEYTDPTIISSVTLKYSAPNQGLGQIYNKDKLKDKLTPSYAFPFFCWGKRYSGKSKRW" }, "paralog": false, "identity": 0.7381818181818182, "score": 414.0, "evalue": 7.4e-149, "description": "Internal stop codon at 526207, 526738. Frameshift due to deletion around 525982. Nonsense mutation around 526207, 526738.", "psc": { "uniref90_id": "UniRef90_P75339", "product": "Uncharacterized lipoprotein MPN_439", "uniref50_id": "UniRef50_P75339", "go_ids": [ "GO:0098552" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75339", "UniRef:UniRef50_P75339", "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75339", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75339" ], "product": "Uncharacterized lipoprotein MPN_439" } }, "id": "GIHJHMOALN_945", "locus": "GCA009939745_00930" }, { "type": "cds", "sequence": "contig_1", "start": 527021, "stop": 527704, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATGCAACAAAAAATGCAAAGTACCATGCAAGATAGTAATCATTATGGTTTTACCGGGCTAAACACCAGTACTAGTTCAATGTTAGGTGCCGCTACGCAGCAAGCCATTTTGGATCAAATAACCAAGAATTCCTTGCAACAATATGGTTCGCAGCAAGAACTGAAAACTTTGATTGAAAAGACTAATAACCAACTTTTATTAGACCGAATCGCTAGTCAATTAAGTGGATTAAATCCTTCAACCACTGGAAATAGTAATAATGGAAAGGGTAAAAATATTGCTACTTATTTCCAATTAGATGCCATTGGCAACCCAACCCTTAGCTTTCAACAAAAACGAAAACTTTTATTAGATGTTTTAGATCAGTACAAAGATTTCTTTGGTACAAATACTCAGGCTGCACAAAGAGATTCTGGTAAAGGTGGACATGGAAGCTATTCAACTTACCAAGATGGCAGTGACAAGATCACTTACCTTCAGTTCTCCTATAAAGATATAGATAACTTAAGTTTGAGTGATAAAGGAAATAGTAAGCTTGCTAGCGATGTTGTGGCTGCCCTTTTACTTTTCCAAGCAGCGGATAAAGGTACCCAACAATTAGCCTTGAGTGCTATTAATAAACCACAACTACCAATTGGTGATAAGCGGATTAAAACAGGAATAGATTTACTTAAATAG", "aa": "MVMQQKMQSTMQDSNHYGFTGLNTSTSSMLGAATQQAILDQITKNSLQQYGSQQELKTLIEKTNNQLLLDRIASQLSGLNPSTTGNSNNGKGKNIATYFQLDAIGNPTLSFQQKRKLLLDVLDQYKDFFGTNTQAAQRDSGKGGHGSYSTYQDGSDKITYLQFSYKDIDNLSLSDKGNSKLASDVVAALLLFQAADKGTQQLALSAINKPQLPIGDKRIKTGIDLLK", "aa_hexdigest": "a6a0378525066007380e86d7be863627", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24783.535799999987, "isoelectric_point": 9.097665596008301 }, "id": "GIHJHMOALN_946", "locus": "GCA009939745_00931" }, { "type": "cds", "sequence": "contig_1", "start": 527966, "stop": 528118, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTGATAAATCCATTTATGGAAATCCTAAGGACAACAAATTTGATGACTTATTTAAATTAGCTTTTGAAGATAGTATTAACGAAAAGTCCTTTAATGTTGATTACAAAGCGGTCATTGAACACTACCGCTTTATCTATACCTTGGAATGA", "aa": "MTDKSIYGNPKDNKFDDLFKLAFEDSINEKSFNVDYKAVIEHYRFIYTLE", "aa_hexdigest": "6489b238ee66db2e3891a3bff0400f39", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6013.649899999999, "isoelectric_point": 4.9303506851196275 }, "id": "GIHJHMOALN_947", "locus": "GCA009939745_00932" }, { "type": "cds", "sequence": "contig_1", "start": 528125, "stop": 528307, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTCAAACAAACGTTTGACCAAAACGACTTGTTTAAGAAAGCTAAGAAACTTTTTGAAGATATTCAAAGTAAAACAAATGGTGGAAGTTCAGGTGGGATGCAAGGTAGCAATACCTCGAGTAGTGAAGGAGCTGATGCCTTGAGTAAAGTAATTGGCAACTATTACTACAACAGTTGA", "aa": "MLLKQTFDQNDLFKKAKKLFEDIQSKTNGGSSGGMQGSNTSSSEGADALSKVIGNYYYNS", "aa_hexdigest": "162176d8455fd8b24da4fbb08368e614", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6501.096200000004, "isoelectric_point": 7.9830717086792005 }, "id": "GIHJHMOALN_948", "locus": "GCA009939745_00933" }, { "type": "cds", "sequence": "contig_1", "start": 528374, "stop": 528907, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAGGCAAATTTTGATCAAAAGGATAAAGCAAACAAAGCTTTAACTGAAGCCTTAAAGCCTTACAAGGATTTACTGGCAGAATATGCACCAATTGGTTTGGCCACAACCATGATTAACTTCTTGTTTAACCAAATGAACGCGTTCAATAAGTTTTTGGAGCGCGCTAAAGCATTAAACCAAAATGTGAATCAGGCCAATCCTACACCTTGGTTAAATGGTTTATCAGCTAAATTGCCTTATGTTAGAACAAATGGTAATTACGAAAAACTCAACAATTACTTCACTTTCTTAATTGTTAAATACATGTGGAAGAAGGTCGGTAATGAAGACGCTTCATTATCTAAAGATAGTAGCACTAACAAGCTCAAAACTAAGACCGAAGACGTTAACAAAATTAGAGATAAAATCTTAGAAGACATTCAAAAGAAGGTTCAAGAATTTGTTAAAAACAAGCTTAAACCAACCTTAGCACCACGACAAACTTACAGTAATGTAATTTTGTTAAACGTTAACAACGATAAGGTTTGA", "aa": "MVKANFDQKDKANKALTEALKPYKDLLAEYAPIGLATTMINFLFNQMNAFNKFLERAKALNQNVNQANPTPWLNGLSAKLPYVRTNGNYEKLNNYFTFLIVKYMWKKVGNEDASLSKDSSTNKLKTKTEDVNKIRDKILEDIQKKVQEFVKNKLKPTLAPRQTYSNVILLNVNNDKV", "aa_hexdigest": "cdf9a0eef25e221c927749cd8536c321", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20337.24010000001, "isoelectric_point": 9.718044853210447 }, "id": "GIHJHMOALN_949", "locus": "GCA009939745_00934" }, { "type": "cds", "sequence": "contig_1", "start": 528944, "stop": 529399, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACAATCTGATGAAAAATATGTGTTATTAGATGCTGCGATCCCGGCGTTTGAAACTTCTTCACAATCACCACAAAGCAGTGGAACTATTTCTGGTAGTGGCGCTTTAGTTAATTTGACTTCTACTAGCGTCAATTTAACTCAACAAAAAAGTAGTACAACGCCTTTAAACCAGCGCATTGTTCGAAATAGTACTGGTGTAAAATTGCTGGCTCTTGAAGGCTTGCTTTACTACGTTGTTGGATCTTCCAATAAACAACAAAGTAGCTCTTCTACGATAGCCACTTCTCTCGCTCAAAACCAAAGTAATAATAATCAAGAACAAGAGATTAATAGCAGTCTTCAAAAAAATTTATTGAAGTTTCGTGCCTTTCAAGCTAAAAATAGAGTTTTCAAACAAGGCGGCACCAAATATGCCTTTTCGTTTCCGTTAAATGAAACCTTGAAAGGTTGA", "aa": "MAQSDEKYVLLDAAIPAFETSSQSPQSSGTISGSGALVNLTSTSVNLTQQKSSTTPLNQRIVRNSTGVKLLALEGLLYYVVGSSNKQQSSSSTIATSLAQNQSNNNQEQEINSSLQKNLLKFRAFQAKNRVFKQGGTKYAFSFPLNETLKG", "aa_hexdigest": "cd443c0a8be3ca122be5bb461a415f76", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16270.939899999985, "isoelectric_point": 9.742027091979981 }, "id": "GIHJHMOALN_950", "locus": "GCA009939745_00935" }, { "type": "cds", "sequence": "contig_1", "start": 529436, "stop": 529681, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGACACCATTGTAGCTAAATCTGATAACTTAGAGAAAACTTCTAAAACTAACAGTACTTTTTACAAAGATAATGGTGATAGTAGCGAAAAACAAGGAAGTGCTGAAAGCAAAAATTCACCTAAAACTCAATTAAAGGAAATTTTTAATGGTCAAAATGGCGATAGTGGTAATTTAGGTAAGCTAGCTGAACAGTTACAAAAAGCTGACAGTACTAAAATGGAAGCTAGAAGTCAGACATGA", "aa": "MNDTIVAKSDNLEKTSKTNSTFYKDNGDSSEKQGSAESKNSPKTQLKEIFNGQNGDSGNLGKLAEQLQKADSTKMEARSQT", "aa_hexdigest": "4fb514a8a4301a9f7423220b1822eac3", "start_type": "TTG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8802.437799999998, "isoelectric_point": 6.217185401916503 }, "id": "GIHJHMOALN_951", "locus": "GCA009939745_00936" }, { "type": "cds", "sequence": "contig_1", "start": 530394, "stop": 530777, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_442", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "nt": "TTGTTGGGTGGCCTTTCCACTAGTAGTATTATTCTCAGTGCCTGTGCAACACCTTCAAACTCAGCACTGCAAGCAGTTTTTAAACCAACTTCCAACCAATTTTTTAACGGTGAGCATGGTACCATTCAAAGCGCTTTAAACACCGCTTTAAGAGATCCGGAAACTAACAAAAAATTTGTAGCTGCTCCACTTTTAAAAGCATTAGAAGCTTGGTACGAGAATAATCAAGATAAAAACATTACTCAATTCTTAAAAGACACCAAGACTAATGTTGATAACCAATACAAAACCGTTGTAGATAAAGTAGTTTCAGCACCACGCAATAAATCCTTATTTGTACAACAAGATTTATTGGACAGTAGCGGTGGTAGTGAGGCCACTTGA", "aa": "MLGGLSTSSIILSACATPSNSALQAVFKPTSNQFFNGEHGTIQSALNTALRDPETNKKFVAAPLLKALEAWYENNQDKNITQFLKDTKTNVDNQYKTVVDKVVSAPRNKSLFVQQDLLDSSGGSEAT", "aa_hexdigest": "3c229614f401cf9a42d083d70b5c7567", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75336", "query_cov": 0.9606299212598425, "subject_cov": 0.8079470198675497, "identity": 0.762, "score": 182.0, "evalue": 4.37e-57, "valid": false, "product": "Uncharacterized lipoprotein MPN_442", "uniref50_id": "UniRef50_P75336", "go_ids": [ "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "product": "Uncharacterized lipoprotein MPN_442" }, "genes": [], "id": "GIHJHMOALN_952", "locus": "GCA009939745_00937" }, { "type": "cds", "sequence": "contig_1", "start": 530980, "stop": 532011, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_440", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75338", "UniRef:UniRef90_P75338" ], "nt": "TTGGCTTTTGACAGTAGTATTAACGAAAAGTCCTTTAATGTAGATTATAAGGCAGTGATTGAACACTACCGCTTTATCTATACCTTAGAGTGGTTGGTAGACAAAAACCTAAAGAACTTCAAGGATTTATTAAAGGCAAACCTCAAGTTTGGTGAAATTGCTTTTATTGCTTACAAGAATACTGAAACGCAGAACTTCTCTAACCCGCAAGGTATATTCGGTTCTTACTTTAATTACGAAAACGAAACGAATGCGGCTAAGAGTGCTACGCAAATTATAGACCCCAACAGTTTCTTCTATAAAACCACCACTAAACCAGAAGCGAAAACCACTCAAAGTGCTAATACAGCTGTGATGGTTCAAAATACGCAGATGAACAATCAGCAAACTAACAGTTATGGCTTTACTGGTTTGAGTACCAGTAGTGGTTCGATGTTAGGTGCTGCTACCCAGCAAGCCATTTTGGATCAAATAACCAAAACTTCCTTGCAACAGTATGGTTCTCAAGCTGACCTGAAAAAGATCATTGGCGAAACTAAAAATCAATTATTATTAGACCGAATTGCCAACCAACTAATAGCCTTAAAACCGAATACAAGTGGCAACAGTGGTACCCAAAAAACAATTGCTGCATACTTCCAAACAGATGCGGTTGGCAATCCTACTTTGGACTTTAAAGCGAAGCAAAAACTCTTATTGGATGTTTTAGATCAATACAAAGATTTCTTTGGTAATAATGCACAAGCAGTTCAAAGAGATTCTGGTAAGAGTGGAACTGGCAACTATTTAACCTATACCGACGGTAGTGATAAGATCACTTATTTGCAGTTTTCCTATAAAGATATTGACGGTTTAAGTTTGAGTAGTTCAAACGGAACTAGCAGCAAATTTGCCAGTGATGTTGTAGCAGCGCTTTTATTATTCCAGGCAGCCGATAAAGGTACTCAACAACTGGCTTTAAGTTCCATCAATAAACCACAATTACCAATTGGCGATAAACGCATAAAAACAGGGATCGATTTACTGAAATAG", "aa": "MAFDSSINEKSFNVDYKAVIEHYRFIYTLEWLVDKNLKNFKDLLKANLKFGEIAFIAYKNTETQNFSNPQGIFGSYFNYENETNAAKSATQIIDPNSFFYKTTTKPEAKTTQSANTAVMVQNTQMNNQQTNSYGFTGLSTSSGSMLGAATQQAILDQITKTSLQQYGSQADLKKIIGETKNQLLLDRIANQLIALKPNTSGNSGTQKTIAAYFQTDAVGNPTLDFKAKQKLLLDVLDQYKDFFGNNAQAVQRDSGKSGTGNYLTYTDGSDKITYLQFSYKDIDGLSLSSSNGTSSKFASDVVAALLLFQAADKGTQQLALSSINKPQLPIGDKRIKTGIDLLK", "aa_hexdigest": "00e45c316bfb5cdbf56c8f155aa2e7d5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 530980, "stop": 532311, "observations": { "mutation": [ 532080, 532302 ], "stop": [ 532080, 532302 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75338", "query_cov": 1.0, "subject_cov": 0.4724517906336088, "identity": 0.9940000000000001, "score": 649.0, "evalue": 6.13e-229, "gene_start": 1, "gene_end": 343, "reference_start": 384, "reference_end": 726, "reference_sequence": "MQQQGETKDQYNTFGLRLVRNSVGVSVLGLDGFVKFIKGGSGGSNGGSSSAKKIDKEEQKKFLKFRAFQAKIGTFYNTNFAFSFPLNETLKGWFDKHRGLILANALVKVTLDTKEKASKALVDAFSSYKNWLSEYTPVGLATTMISFYFDQMKALNNKLLERVRSLNQNVNQANPTPWLNGLSAKLPYVNTNGNYEKLNNYFTFLITKVLWPKVGTEDTNVSEEKSKLKTKTEDVNKIREKILNNIDSKLKTFVQKLKPTLAPRPAYSNVILLNINNDKVWSAGANWSLAVLLDPKKVNPLSFMLLKQMFDQNSLFKKAKTLFENIQNKAKTSGSGKSGTTTNDDADALSKVIGNYYYNTWAKLTDKSIYGNLKDDKFDDLFKLAFDSSINEKSFNVDYKAVIEHYRFIYTLEWLVDKNLKNFKDLLKANLKFGEIAFIAYKNTETQNFSNPQGIFGSYFNYENETNAAKSATQIIDPNSFFYKTTTKPEAKTTQSANTAVMVQNTQMNNQQTNSYGFTGLSTSSGSMLGAATQQAILDQITKTSLQQYGSQADLKKIIGETKNQLLLDRIANQLIALKPNTSGNSGTQKTIAAYFQTDAVGNPTLDFKAKQKLLLDVLDQYKDFFGNNAQAVQRDSGKSGTGNYLTYTDGSDKITYLQFSYKDIDGLSLSSSNGTSSKFASDVVAALLLFQAAYKGTQQLALSSINKPQLPIGDKRIKTGIDLLK" }, "paralog": false, "identity": 0.9909706546275395, "score": 855.0, "evalue": 1.75e-312, "description": "Internal stop codon at 532080, 532302. Nonsense mutation around 532080, 532302.", "psc": { "uniref90_id": "UniRef90_P75338", "product": "Uncharacterized protein MPN_440", "uniref50_id": "UniRef50_P75338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75338", "UniRef:UniRef50_P75338" ] }, "pscc": { "uniref50_id": "UniRef50_P75338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75338" ], "product": "Uncharacterized protein MPN_440" } }, "id": "GIHJHMOALN_953", "locus": "GCA009939745_00938" }, { "type": "cds", "sequence": "contig_1", "start": 532078, "stop": 532296, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGTTTTACTAGATCCGAAAAAGGTTAACCCGCTTTCGTTTATGCTGCTCAAACAAATGTTTGATCAAAACAGTTTGTTTAAAAAAGCAAAGACTTTATTCGAAAACATTCAAAATAAAGCAAAAACTAGTGGAAGTGGTAAAAGTGGTACAACCACCAACGATGATGCCGATGCTTTGAGCAAAGTCATTGGCAACTATTACTACAACACTTGA", "aa": "MAVLLDPKKVNPLSFMLLKQMFDQNSLFKKAKTLFENIQNKAKTSGSGKSGTTTNDDADALSKVIGNYYYNT", "aa_hexdigest": "db33fac5edd172cc0438b4f386071f20", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7995.079200000001, "isoelectric_point": 9.477771186828615 }, "id": "GIHJHMOALN_954", "locus": "GCA009939745_00939" }, { "type": "cds", "sequence": "contig_1", "start": 532300, "stop": 532521, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCACTGAGGATACTAATGTCAGTGAAGAAAAAAGCAAACTCAAAACTAAAACTGAAGATGTTAACAAGATTAGGGAAAAGATTTTGAACAACATCGACAGTAAGCTCAAAACTTTTGTCCAAAAACTCAAACCTACCTTAGCACCCCGACCAGCTTACAGTAACGTAATCTTGTTAAACATTAACAATGATAAGGTGTGGTCTGCTGGCGCTAATTGA", "aa": "MGTEDTNVSEEKSKLKTKTEDVNKIREKILNNIDSKLKTFVQKLKPTLAPRPAYSNVILLNINNDKVWSAGAN", "aa_hexdigest": "ffd08ece77589e5777c47a77399b7a75", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8214.322999999999, "isoelectric_point": 9.522189903259278 }, "id": "GIHJHMOALN_955", "locus": "GCA009939745_00940" }, { "type": "cds", "sequence": "contig_1", "start": 532528, "stop": 532767, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTGAATACACCCCGATTGGTTTAGCTACCACCATGATTAGCTTTTATTTTGACCAAATGAAAGCTCTCAACAATAAGCTGTTAGAACGAGTACGGAGCTTAAACCAAAATGTTAACCAAGCCAATCCTACCCCTTGGTTAAATGGGTTATCGGCTAAACTACCTTACGTTAACACTAACGGTAATTACGAAAAGTTAAACAACTACTTTACCTTTTTAATTACCAAAGTTTTGTGA", "aa": "MSEYTPIGLATTMISFYFDQMKALNNKLLERVRSLNQNVNQANPTPWLNGLSAKLPYVNTNGNYEKLNNYFTFLITKVL", "aa_hexdigest": "d0f94f7a39f9b25156f0ab0375b712cd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9092.348699999997, "isoelectric_point": 9.397701454162597 }, "id": "GIHJHMOALN_956", "locus": "GCA009939745_00941" }, { "type": "cds", "sequence": "contig_1", "start": 532768, "stop": 533160, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAGCAAGGAGAAACTAAAGATCAATATAACACTTTTGGCCTGAGACTTGTGCGTAACAGTGTTGGCGTATCAGTTTTAGGACTTGATGGTTTTGTTAAATTCATAAAAGGCGGTAGCGGAGGTAGCAATGGCGGTTCTAGTAGTGCCAAAAAGATTGATAAAGAAGAGCAAAAGAAATTCTTAAAGTTCCGTGCTTTTCAAGCCAAAATTGGCACATTTTATAACACTAACTTTGCCTTTAGTTTTCCCCTAAACGAAACTTTAAAAGGTTGGTTTGACAAACACCGCGGATTGATCTTAGCGAACGCCTTAGTTAAAGTTACTTTAGACACAAAGGAAAAAGCAAGTAAGGCCTTAGTGGATGCTTTTAGTTCTTATAAAAACTGA", "aa": "MQQQGETKDQYNTFGLRLVRNSVGVSVLGLDGFVKFIKGGSGGSNGGSSSAKKIDKEEQKKFLKFRAFQAKIGTFYNTNFAFSFPLNETLKGWFDKHRGLILANALVKVTLDTKEKASKALVDAFSSYKN", "aa_hexdigest": "526b1f0da89f6d803e10eacb4e5892cd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14354.209699999976, "isoelectric_point": 9.910160636901853 }, "id": "GIHJHMOALN_957", "locus": "GCA009939745_00942" }, { "type": "cds", "sequence": "contig_1", "start": 533167, "stop": 533910, "strand": "-", "frame": 1, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W962", "UniRef:UniRef90_A0AB38W962" ], "nt": "ATGAAATTGCTGGGAGTCAAAATGTCGGCAATAAACGCTGAAAACAACTGGTTAAAGGTGAAGAAGCGATTGAAGAAAAGCCAAAGCAACAACAAGATGGCAGTCAAACAAAAGGAGTTCAAAAAAGAAGGCCTTACCAAGTATGTACGGGGAACCGACAGAGGCCAAGTAATTGACTTTCCGTTAAGCTTATCAGATACTAACGAGACCAAAAAAGTCGTGGATGCCACCAACATTGTCGATCAATTAGAGGCAGCTGATTTAGCCGCTGTGCTCAACATTAAACTAGACCGACTGGGTCAAGAGAAGGATGAGCTACTCGAATTTAAGGAGCTCAAAGATGACCTTAACAACGTGATTGTGATTGATAAGAGTAGTGATGCACAAAATGTGAGCAAAACTAATTCTTCTTTTTATGAGGATAATCAAAACTCTGGAACCACAAATACCGATCCCATTGCCCAAGCTTTAGATGACATCTTTGCCACAAACGTGGAACAAAAAACTAATCTAGGCAAGTTAGCAGAACAAATTAAAAAGACAGCGGAAATTAAAACGCAAACCCCACAAGCAAGAGGCACTGTTGTTTTGCGAAATAAATCGCAAACTCAACAAAAGAACAGTTATGTTCTATTAGATGCTGGTATTCCTACCTTTAAACAGCAAGTCAGCACCCAGGGTCAAGGTTCAGTGAGTCTTCATAACGGTACCTTAATTTCTTTAAACACATCTACAGTTGCCTAA", "aa": "MKLLGVKMSAINAENNWLKVKKRLKKSQSNNKMAVKQKEFKKEGLTKYVRGTDRGQVIDFPLSLSDTNETKKVVDATNIVDQLEAADLAAVLNIKLDRLGQEKDELLEFKELKDDLNNVIVIDKSSDAQNVSKTNSSFYEDNQNSGTTNTDPIAQALDDIFATNVEQKTNLGKLAEQIKKTAEIKTQTPQARGTVVLRNKSQTQQKNSYVLLDAGIPTFKQQVSTQGQGSVSLHNGTLISLNTSTVA", "aa_hexdigest": "1042874f348cd24c962b98227c35d4bb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W962", "query_cov": 1.0, "subject_cov": 0.96484375, "identity": 1.0, "score": 449.0, "evalue": 7.75e-159, "valid": true, "product": "Protein of unknown function (DUF3713)", "uniref50_id": "UniRef50_A0AB38W962" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W962", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W962" ] }, "genes": [], "id": "GIHJHMOALN_958", "locus": "GCA009939745_00943" }, { "type": "cds", "sequence": "contig_1", "start": 533870, "stop": 534271, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU13", "UniRef:UniRef90_A0AB33HU13" ], "nt": "TTGTTTGAACAGCTCATTAGCGATTTTGCTAGTCGGGTCTTTCAAAAGAACTACCTCGCTTATAAGGATGGTAATAAGGTATCATCAGGTCCTTTCTTATTTGACGAAATTTCTAAATCGAGCAATTGGGACAAAATTGTCTTTGATGCGGGCCAGTTTCCCGAAATCAATGATGCCTTCTTTGCCCAGATTCAAAGCGAAGTTTTCGATCAATGGGCGGAATACACCGATCCCACTGTTATTAGTTTCACTACCTTAAAGTACTCGGCTCCTAACCAGGGATTAGGGCAAATCTATAACCAGGACAAACTAAAGGATAAATTAACCCCTTCCTATGCCTTTCCGTTTTTTGCTGCAGCGGATGAAATTGCTGGGAGTCAAAATGTCGGCAATAAACGCTGA", "aa": "MFEQLISDFASRVFQKNYLAYKDGNKVSSGPFLFDEISKSSNWDKIVFDAGQFPEINDAFFAQIQSEVFDQWAEYTDPTVISFTTLKYSAPNQGLGQIYNQDKLKDKLTPSYAFPFFAAADEIAGSQNVGNKR", "aa_hexdigest": "bd4544cde609fd15eacd3da6158bab46", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 533708, "stop": 534333, "observations": { "deletion": [ 534303 ], "stop": [ 533736, 533871, 534285 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HU13", "query_cov": 1.0, "subject_cov": 0.6394230769230769, "identity": 0.992, "score": 272.0, "evalue": 1.71e-91, "gene_start": 1, "gene_end": 133, "reference_start": 22, "reference_end": 154, "reference_sequence": "MSNKTCWIAPGGGLETWKAQKLFEQLISDFASRVFQKNYLAYKDGNKVSSGPFLFDEISKSSNWDKIVFDAGQFPEINDAFFAQIQSEVFDQWAEYTDPTVISFTTLKYSAPNQGLGQIYNQDKLKDKLTPSYAFPFFAAADEIAGSQNVGNKRWKQLVKGEEAIEEKPKQQQDGSQTKGVQKRRPYQVCTGNRQRPSNWLSVKLIRY" }, "paralog": false, "identity": 0.9807692307692307, "score": 406.0, "evalue": 8.25e-147, "description": "Internal stop codon at 533736, 533871, 534285. Frameshift due to deletion around 534303.", "psc": { "uniref90_id": "UniRef90_A0AB33HU13", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AB33HU13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HU13", "UniRef:UniRef50_A0AB33HU13" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HU13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HU13" ], "product": "Lipoprotein" } }, "id": "GIHJHMOALN_959", "locus": "GCA009939745_00944" }, { "type": "cds", "sequence": "contig_1", "start": 534284, "stop": 534466, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGCTTGGTATGAAAATAACCAAGACAAACAGGTCACCCAGTTCTTTAAAGACACCAAAAAGAGTGTTGATGAGCAATACAACCAAGCCGTAGATAAGGTGGTATCGGCTAGCCGAAACAAAAACCTCTTTGTCCAACAAGACTTGCTGGATAGCGCCGGGGGGGGGTTAGAAACTTGA", "aa": "MTAWYENNQDKQVTQFFKDTKKSVDEQYNQAVDKVVSASRNKNLFVQQDLLDSAGGGLET", "aa_hexdigest": "d8b650e4ef43004052478233f4f2c679", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6829.399000000003, "isoelectric_point": 4.764949226379394 }, "id": "GIHJHMOALN_960", "locus": "GCA009939745_00945" }, { "type": "cds", "sequence": "contig_1", "start": 534703, "stop": 535035, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAGCTTTTACATCCACCGTTCTGGTAGAGTAGCGCGTTTTAACAGTTGGGGCAATTCCTATGTGTTTGATTTACCGCATAACCAACACAAATTAACTAAGTTTGCCCATAAAGGTATTTTGTTTAACAGTGTTCATTTAGAACGTGATGGTACTTTAAAGTTCCCCCAACTTAAACAAAGCAAACAAAAACCAGCAGTATCCAGTGAACTTAAAACTGCCATTAAGCGGATTAAGGCAGGATACAAGAAGGTCAAGCCTAACTATAAGAAGCGCCAAAAGCAGCAGATTGCGGAACTCTTTGCCAAGCGCAAACAACGCCGTTCATGA", "aa": "MDSFYIHRSGRVARFNSWGNSYVFDLPHNQHKLTKFAHKGILFNSVHLERDGTLKFPQLKQSKQKPAVSSELKTAIKRIKAGYKKVKPNYKKRQKQQIAELFAKRKQRRS", "aa_hexdigest": "78f6aee323532ab0e972ed95b1855ea9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12969.92439999998, "isoelectric_point": 10.869127845764158 }, "id": "GIHJHMOALN_961", "locus": "GCA009939745_00946" }, { "type": "cds", "sequence": "contig_1", "start": 535048, "stop": 535350, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37" ], "nt": "GTGAAACACTTTCTGCTTAACCTCAACGGTCAAGAACCGTTTGCTGGTTTATTAGCTTTACTCAACTACCACCATAACGAACAGGTCTTGGTCTTTTGTTCCAACGCTAAATCACTCAAACAACTCACTAGTTTACTAGCCCACAACCAAGTTAGCTTTAAAAGCCTCCATGGTCAACTAACGCCCAATGAGCGCAAACACATCTTTACTAGTGCCGCTAATAACACGGTGAGGGTACTAGTGGTATCTGACTTACTAGCACGCGGCATTGATCTGCCCCACTTTAGTGTGGTCATTTCATGA", "aa": "MKHFLLNLNGQEPFAGLLALLNYHHNEQVLVFCSNAKSLKQLTSLLAHNQVSFKSLHGQLTPNERKHIFTSAANNTVRVLVVSDLLARGIDLPHFSVVIS", "aa_hexdigest": "b110d01ea686b7a5cb8275d2b90feb1a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 83, "aa_cov": 0.83, "hmm_cov": 1.0, "evalue": 2.0167399503800276e-19, "score": 65.38690185546875, "start": 17, "stop": 99 } ], "seq_stats": { "molecular_weight": 11126.746099999988, "isoelectric_point": 9.428775215148928 }, "id": "GIHJHMOALN_962", "locus": "GCA009939745_00947" }, { "type": "cds", "sequence": "contig_1", "start": 535363, "stop": 535818, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35" ], "nt": "GTGGTTGGCATAGCCGAAACTGGTAGTGGTAAAACATTCGCTTACCTCTTACCAGCGCTCAACCAAATTGATGTCACGCTCAACAAGCCCCAAGCGGTGGTGTTTGTACCAACTAAGGAGTTGAAGTGGCAAATTTTAACGATCCTCAACGACATTAAAGCCAGCTTTACTGGTTTAAAGGTCAGTGAGAACTTCAACAGTAATGCCCACCTGATTGTGAGCTTAATTGGCAAAGACATTATTCTCCACGAAACGATTAAGTACGTTGTCTTTGATGAGGTCGACATGTTTTTGGAGGACAGTTCCCAAGCAGAGTGGATTAACTGTGTCCAACTGTTTCAAAAACATAAACCTCACTTTGGCTTTTTTAGTGCCACGTTATTTAACGAACAGCTCCAGCAAATTCGCAAGCAAGTCGCACCTTTAACGGTCGTCAACCAAAAGAAACGTGCTTGA", "aa": "MVGIAETGSGKTFAYLLPALNQIDVTLNKPQAVVFVPTKELKWQILTILNDIKASFTGLKVSENFNSNAHLIVSLIGKDIILHETIKYVVFDEVDMFLEDSSQAEWINCVQLFQKHKPHFGFFSATLFNEQLQQIRKQVAPLTVVNQKKRA", "aa_hexdigest": "eb333483a33dc08916a189b699169f10", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 152, "aa_cov": 0.8874172185430463, "hmm_cov": 0.9868421052631579, "evalue": 1.6291310306409567e-25, "score": 85.22837829589844, "start": 1, "stop": 134 } ], "seq_stats": { "molecular_weight": 17089.640799999994, "isoelectric_point": 7.862386894226074 }, "id": "GIHJHMOALN_963", "locus": "GCA009939745_00948" }, { "type": "cds", "sequence": "contig_1", "start": 535927, "stop": 536946, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACCGGGCTGCTGATGACACTTCAAGCTTCTTCTACACTAAGTCTAGTAGTAGTACTGGCGCAGCACAAAGTAGTGCCACTGTTTTAAGGAGTTTAAATCAAGCTAGTGGCATGACCACAAAAACTGCCAAAAACCGCTATGGCTTTAGGGGTATTGTAACGAGTAGCACTAGTGGTAGTTTACCCGAAGCGGTAAGTAGACGTTTGTTTAAGCAGTTTGTTAACCAAACAGAAAAAGGCGTTAAAGTTGGTGGTCAAATGCTAATTACCACAGCTAAAAGTGGTAAAGCTACCTTAGTTTTAAAACAACAAGCTGACGACGCCGAATCCACCACCAACAACGCTTACAAAGGTGCCTTGTTTAGCTTTGGTTCGATGGATAACTTAAAGAACATCATCAACGGAATCCAAACGCAAACCGAGTTCGATGCGCTTTACAACCACTTAACTAGTGACCTCAACATTGATGTTACTGGGGTTGATAAAAACAAAACTTTAACGGAACAGAAAACTAGTTTAACGAGTTTTGTTGATTCTAACTTTAAGCAGAGTACTCAATCTGCACAGAGAGGCGATACAAGTGCACGCAGTGCAAGATCTGCAACAGTACAAATTAAGAAAACACAAGAAGATAACCAAAATACCAACTATAAAGACGTCTTTAGCCGCTTTGATGGTTACATTGGTGATAACAAGGTAGAGGAAAAGAACTACACTTCCTACCAATTCTTAAGTGACGGTGGCAAATACCACGCTACCTTTGTCAAACAGGTCAACCTTGATGATGTGGAGAAGATCGGCACTGATTCTCTAAAGCAAGAAGATTCCAGCAAAGACAAACGCCTCAACCTCTCCCTCGAAGAATTCCTCGCTGCAATTGCGCTAGAAGCCTTGGATCCTAATAATCAAACCCAAGCCATTAACGCCTTAATTAGTGGTAATAAGAAGGGTTTAGTTAAAGTAGGTGACTTTAGGATCTTTAGTTCTATTAGTGCGCAATGGGTAAGACGTTTTTAA", "aa": "MNRAADDTSSFFYTKSSSSTGAAQSSATVLRSLNQASGMTTKTAKNRYGFRGIVTSSTSGSLPEAVSRRLFKQFVNQTEKGVKVGGQMLITTAKSGKATLVLKQQADDAESTTNNAYKGALFSFGSMDNLKNIINGIQTQTEFDALYNHLTSDLNIDVTGVDKNKTLTEQKTSLTSFVDSNFKQSTQSAQRGDTSARSARSATVQIKKTQEDNQNTNYKDVFSRFDGYIGDNKVEEKNYTSYQFLSDGGKYHATFVKQVNLDDVEKIGTDSLKQEDSSKDKRLNLSLEEFLAAIALEALDPNNQTQAINALISGNKKGLVKVGDFRIFSSISAQWVRRF", "aa_hexdigest": "7c26ad8d8078b8382ec0416d93a10d3b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37203.80570000003, "isoelectric_point": 9.271343421936034 }, "id": "GIHJHMOALN_964", "locus": "GCA009939745_00949" }, { "type": "cds", "sequence": "contig_1", "start": 536968, "stop": 537177, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAGCGAAAAGAATAAAAATGATTCACAATCAACTTTATCCAGTACCGCTTCAAGCACAAGTAATACTGGCTTAATTCAGTTAAGATCAGTCGTTTCCCTAGCACAAAATCAAGCAGCTGGCCAAGGCGGTGATAATAATAGTGACATCACCCAAACCGAAGTCAAAAACCCGAACTTTGTGTTTGGTTCGAGTGTGTATGATTGA", "aa": "MDSEKNKNDSQSTLSSTASSTSNTGLIQLRSVVSLAQNQAAGQGGDNNSDITQTEVKNPNFVFGSSVYD", "aa_hexdigest": "c53e9fdcbed1ac14f7763e28c1f4e525", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7242.607900000003, "isoelectric_point": 4.4446615219116214 }, "id": "GIHJHMOALN_965", "locus": "GCA009939745_00950" }, { "type": "cds", "sequence": "contig_1", "start": 537181, "stop": 537450, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACTTATTTGAATCACAAGCCCAAAAATCAATCTTTGGTCATGTTGGTTCATCTGATAAAAATTCTTCAACTAAGACGGGCATCGAAGGTGTCTTGGACACACTGTACAGCTCGCTAAACTTAGAAGAGCGCTTAGATAGTGATGATGTAATTGATTATCTGTCTTACCTCTACACGGCCCATTGGTTGTTAAAGGACAACTTAAAGAACTATAAGCAAAGCTTACAGTCGAAGTTGTCGCGTACTTCCAATGCCTTTTTAGTTTGA", "aa": "MDLFESQAQKSIFGHVGSSDKNSSTKTGIEGVLDTLYSSLNLEERLDSDDVIDYLSYLYTAHWLLKDNLKNYKQSLQSKLSRTSNAFLV", "aa_hexdigest": "a4d773122e2f7ea61da954a7f99a45c4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10083.09349999999, "isoelectric_point": 5.152136421203611 }, "id": "GIHJHMOALN_966", "locus": "GCA009939745_00951" }, { "type": "cds", "sequence": "contig_1", "start": 537475, "stop": 539271, "strand": "-", "frame": 1, "gene": null, "product": "putative lipo MG309-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47551", "UniRef:UniRef90_P75334" ], "nt": "GTGTACGCGGCCATCCAAGACAAACTGTTAGACCAGTTTATTACCAATGAAAACCCCAACTTGGTATCTCGGGTCGTGTTTACCAATGAAACTCCCGTTGACGGGTTTGATAATTACTTTAACACTAAAGTAATTCAAAGTCCAACCCCTTCCTACCAGTTTCAGGTCTTTAACAAATACAACCAACAGAGTGGTGGCACTAAGGGAGCGAATGGCTTTAACTTATTGGCTAGCAACCTCAAGAGTTACAAAAACGACCAGAGCAAAGGGATTGATATTCCCAATAAGTTTTCCAGTGATAGTGGTGGCAAATTGTTGTTAAAGGCGAGCGACATGTTCGACACCTTTGATCCGAGTTTTTCTGCTGCTTTCATTCAGGGCTATTTAGCACTGCAGAAAAAGTCGAAGGGCGCTGATAGTAAAGAAGTTGACAGTCTCATTAAGGATAAGAGTATTATTGAAAACTTTTTTGTTGACAATAATACACAGGCAGCCGCCGCTGCAAGGGCTGCGAGTTCATCATCTGAAGGAACTATTCAATTAAAAACTGCCAGTGATGGTGGTGGAACTACACAAAGTACCGTCCACAAAACTGACTTAGTCAAAATCTTTGGTGATAAAGATGTCTTTGCTGGTGAGTATAAACAACAAATAGGTAATACTAATGCTAATCAAACAGGTGGCGGAGGTTCTGGAGGCGGCGGTGGCACCTCTACCGGCAGTAGCACCGGATCTTCTACTGAAACTACTACTGGCAACAGTAGTAAGGCGGTTGTTGACTTAATTGAAGTTAAAAAAGATTCTAGTAGTCAACCAGACTACATCCTCTCCCGTGGTAAAGACGGCATCCACTTAATGGCCGTGGATGGTGGTAGTCATTATCTTACTGAAATTGGTCGTGATGTCGCTAAACAAAAGGAATTCTTATTGTTCCGGGCTTTGCAAACCAAGTACGGTTTAGTGGATACCGACACCACATACGACTTTAAGTTATTTGATGAGGTCAAAAAGTACTTTGACACTAACCGGATCTTATTCTTATTTGAAGCTTTATTAGATCTTTCTAGTGATACAAACAACAAAGACAACTTCTTAAGCTATCCCCAGTTTAAGAAGTTTGCTGACAGTATTAAGTCAATTGAGAAGGATTTAAAAGAACTTGTTCAAGCCCACTATAAACAAGCAGTCTTTAACGAAACTGCAGTGGCGGAAAACAAGGTTACCTTAAAGCTAGCAGAACGTAACCAACCTTTTATTGACAACGAGCGCAATAACCAGATTGAACAGAACGGTTTGGCAGCTAAGTTACCTTACGAACAAGATGCTAAAACCGGTCACTATAATGACCTTGGTAACTATTACAAGGACATTATTGATAATGTTGATAAAAAAGGAACTAGTACTGTTAAAACAACCTCTTCCAATACAGGACAAACTAAAAACTTCTCTGAAGAAGTAGTTAGCAAGCTTAAAGATAATAAGAAGAAGGTAGAAGAAGCTGCTAAAAAACATGTAGAGGCACTCAAAGTCTTCACCATTCCTTCCCCACTCTACTCCCAGGTAATCTTAGTGCAAACTAAGTTGTCTTTTACCCCTGAAAGTACCTCTTTAGGACTTAACTTAGCTCTAAACAACTATTTAACCAGTACTGAGCTCCAAAACAGCATTAAGCTCAGTTACTTCCAGGAAGATGAAGCGTTTAAAAAGATTATTGATATTACGAATTTAACTTTTAGTCAACAAAGTGGTGGTACTGGTGGCACCAACGGTAACAACAACCTCACCGCTGACAACTGA", "aa": "MYAAIQDKLLDQFITNENPNLVSRVVFTNETPVDGFDNYFNTKVIQSPTPSYQFQVFNKYNQQSGGTKGANGFNLLASNLKSYKNDQSKGIDIPNKFSSDSGGKLLLKASDMFDTFDPSFSAAFIQGYLALQKKSKGADSKEVDSLIKDKSIIENFFVDNNTQAAAAARAASSSSEGTIQLKTASDGGGTTQSTVHKTDLVKIFGDKDVFAGEYKQQIGNTNANQTGGGGSGGGGGTSTGSSTGSSTETTTGNSSKAVVDLIEVKKDSSSQPDYILSRGKDGIHLMAVDGGSHYLTEIGRDVAKQKEFLLFRALQTKYGLVDTDTTYDFKLFDEVKKYFDTNRILFLFEALLDLSSDTNNKDNFLSYPQFKKFADSIKSIEKDLKELVQAHYKQAVFNETAVAENKVTLKLAERNQPFIDNERNNQIEQNGLAAKLPYEQDAKTGHYNDLGNYYKDIIDNVDKKGTSTVKTTSSNTGQTKNFSEEVVSKLKDNKKKVEEAAKKHVEALKVFTIPSPLYSQVILVQTKLSFTPESTSLGLNLALNNYLTSTELQNSIKLSYFQEDEAFKKIIDITNLTFSQQSGGTGGTNGNNNLTADN", "aa_hexdigest": "cf7b5186ef16fc7460e9eba5e499cfb2", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 537172, "stop": 539571, "observations": { "mutation": [ 537183, 537477, 539481 ], "stop": [ 537183, 537477, 539481 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75334", "query_cov": 1.0, "subject_cov": 0.44165435745937964, "identity": 0.998, "score": 1068.0, "evalue": 0.0, "gene_start": 1, "gene_end": 598, "reference_start": 211, "reference_end": 808, "reference_sequence": "MHTFTRKVKWPFMFTAIGLTFGIVAVACAQPTASTVEGLFKPSSAFADRTDFSLSSILQKSLINRESFNQYLAMRLAPVLRTFYDDNYDTDIKERLNGFTADTDNAFVSQEQNLRNQFRENYLVHLQTDIFDNTGGNQAAWKLRDVNNKIIDDFISRIFAKNFVEYVQDGVGPLTKPTKSLIENTSNFKNIKLQPKFVNKNAKLKINNDAVYAAIQDKLLDQFITNENPNLVSRVVFTNETPVDGFDNYFNTKVIQSPTPSYQFQVFNKYNQQSGGTKGANGFNLLASNLKSYKNDQSKGIDIPNKFSSDSGGKLLLKASDMFDTFDPSFSAAFIQGYLALQKKSKGADSKEVDSLIKDKSIIENFFVDNNTQAAAAARAASSSSEGTIQLKTASDGGGTTQSTVHKTDLVKIFGDKDVFAGEYKQQIGNTNANQTGGGGSGGGGGTSTGSSTGSSTETTTGNSSKAVVDLIEVKKDSSSQPDYILSRGKDGIHLMAVDGGSHYLTEIGRDVAKQKEFLLFRALQTKYGLVDTDTTYDFKLFDEVKKYFDTNRILFLFEALLDLSSDTNNKDNFLSYPQFKKFADSIKSIEKDLKELVQAHYKQAVFNETAVAENKVTLKLAERNQPFIDNERNNQIEQNGLAAKLPYEQDAKTGHYNDLGNYYKDIIDNVDKKGTSTVKTTSSNTGQTKNFSEEVVSKLKDNKKKVEEAAKKHVEALKVFTIPSPLYSQVILVQTKLSFTPESTSLGLNLALNNYLTSTELQNSIKLSYFQEDEAFKKIIDITNLTFSQQSGGTGGTNGNNNLTADNWKIFKETYLLDLFESQAQKSIFGHVGSSDKNSSTKTGIEGVLDTLYSSLNLEERLDSDDVIDYLSYLYTAHWLLKDNLKNYKQSLQSKLSRTSNAFLVWSVDSEKNKNDSQSTLSSTASSTSNTGLIQLRSVVSLAQNQAAGQGGDNNSDITQTEVKNPNFVFGSSVYDWTNSKTPEVNRAADDTSSFFYTKSSSSTGAAQSSATVLRSLNQASGMTTKTAKNRYGFRGIVTSSTSGSLPEAVSRRLFKQFVNQTEKGVKVGGQMLITTAKSGKATLVLKQQADDAESTTNNAYKGALFSFGSMDNLKNIINGIQTQTEFDALYNHLTSDLNIDVTGVDKNKTLTEQKTSLTSFVDSNFKQSTQSAQRGDTSARSARSATVQIKKTQEDNQNTNYKDVFSRFDGYIGDNKVEEKNYTSYQFLSDGGKYHATFVKQVNLDDVEKIGTDSLKQEDSSKDKRLNLSLEEFLAAIALEALDPNNQTQAINALISGNKKGLVKVGDFRIFSSISAQWVRRFLNHYALHSTTPSVLTNEAHCWIFIYSTSCV" }, "paralog": false, "identity": 0.9962453066332916, "score": 1484.0, "evalue": 0.0, "description": "Internal stop codon at 537183, 537477, 539481. Nonsense mutation around 537183, 537477, 539481.", "psc": { "uniref90_id": "UniRef90_P75334", "product": "Uncharacterized lipoprotein MG309 homolog", "uniref50_id": "UniRef50_P47551", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75334", "UniRef:UniRef50_P47551", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47551", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47551" ], "product": "Uncharacterized lipoprotein MG309" } }, "id": "GIHJHMOALN_967", "locus": "GCA009939745_00952" }, { "type": "cds", "sequence": "contig_1", "start": 539330, "stop": 539491, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAAAGTTAGAGGTGTTTTCAATTAAGCTCTTGGTGGGTTTAGTTAAAGGACCTACACCGTCCTGGACATACTCGACAAAGTTCTTGGCAAAGATCCGTGAGATAAAGTCATCAATTATCTTGTTGTTAACATCACGCAGTTTTCAAGCGGCTTGA", "aa": "MFLKLEVFSIKLLVGLVKGPTPSWTYSTKFLAKIREIKSSIILLLTSRSFQAA", "aa_hexdigest": "c1eaf45a16f3e6331b3d1fb162cd170c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5972.174799999998, "isoelectric_point": 10.383809852600098 }, "id": "GIHJHMOALN_968", "locus": "GCA009939745_00953" }, { "type": "cds", "sequence": "contig_1", "start": 539479, "stop": 539901, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATACGTTTACGCGTAAAGTCAAGTGGCCCTTTATGTTCACCGCAATCGGGCTCACTTTTGGTATAGTCGCGGTTGCCTGTGCGCAGCCTACAGCCAGCACTGTGGAGGGGCTTTTTAAGCCCAGTAGTGCTTTTGCTGATCGCACTGACTTTAGTCTTAGCTCGATTCTGCAAAAGTCACTAATTAACCGCGAGAGTTTTAACCAGTATCTAGCAATGCGTTTAGCACCCGTTTTGCGCACCTTTTATGACGACAATTATGATACTGATATTAAAGAGCGCTTAAACGGCTTTACCGCTGACACGGATAATGCGTTTGTCAGTCAAGAACAAAACCTACGGAACCAGTTTCGCGAAAACTATTTAGTGCACCTGCAAACTGATATCTTTGACAACACTGGCGGTAATCAAGCCGCTTGA", "aa": "MHTFTRKVKWPFMFTAIGLTFGIVAVACAQPTASTVEGLFKPSSAFADRTDFSLSSILQKSLINRESFNQYLAMRLAPVLRTFYDDNYDTDIKERLNGFTADTDNAFVSQEQNLRNQFRENYLVHLQTDIFDNTGGNQAA", "aa_hexdigest": "6d4051e664a05ea3df7894393d909b56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15877.619099999978, "isoelectric_point": 5.594173240661621 }, "id": "GIHJHMOALN_969", "locus": "GCA009939745_00954" }, { "type": "cds", "sequence": "contig_1", "start": 539956, "stop": 540585, "strand": "+", "frame": 1, "gene": "menH", "product": "Putative esterase/lipase 1", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0016020", "GO:0052689", "SO:0001217", "UniRef:UniRef50_P75333", "UniRef:UniRef90_P75333" ], "nt": "ATGCGGCTCGAAATTGAAAACGGCTTGGAATTTGTGTGTGATCCCTTTTTAAATGAGCGCGGCAAAATCCTTTTTTTACACGCCTTTACCGGTAATATCACCAATAAATTAAGCTTTCGCACCCACTTTAAAGATTACAGTTTTTACGGGATTAACTTTCCCGGACACGGTAACAGTGTCATTCACAACCAAAGTGAATTAGACTTTAACTTCTGGATTAAGTTAGTGCAACAGTTCTTTAATAAATACCAATTAAAGAACGTGGTTTTATTTGGTCACTCGATTGGTGGCGGACTAGCGATTGCCCTCACCCAAGTTTTAACTAAGGAACAAATTAAAGGCATTATCCTCGAAGCGCCCCTAAACCCGGGGATTCGCGCTACCCCACCAAGTATTATTAGTGCCTTGGTACCTGACACCAACGAAGACTTTGAAGCGGTACAAAGGGCATTGATCTACAACATTGAACAACGCTTTGGGGCGAACTTTAAGGACTTTTGTGCTAAACAAAAACAGAAGATGATCCAAAAGTATGCACCCTTAAAGGTAATGTTGCAACCCGAACAAGCCGAACAACGGCTCCAGTTAATTGATGCGGCTTTTAAACGGTTAAGTTACCCAACCCTGTGA", "aa": "MRLEIENGLEFVCDPFLNERGKILFLHAFTGNITNKLSFRTHFKDYSFYGINFPGHGNSVIHNQSELDFNFWIKLVQQFFNKYQLKNVVLFGHSIGGGLAIALTQVLTKEQIKGIILEAPLNPGIRATPPSIISALVPDTNEDFEAVQRALIYNIEQRFGANFKDFCAKQKQKMIQKYAPLKVMLQPEQAEQRLQLIDAAFKRLSYPTL", "aa_hexdigest": "e8ccc20fedc624d2e571c0e4234552d9", "start_type": "ATG", "rbs_motif": null, "genes": [ "menH" ], "truncated": "both", "pseudogene": { "start": 539896, "stop": 540765, "observations": { "mutation": [ 539947, 540583 ], "stop": [ 539947, 540583 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75333", "query_cov": 1.0, "subject_cov": 0.7231833910034602, "identity": 1.0, "score": 414.0, "evalue": 2.81e-145, "gene_start": 1, "gene_end": 209, "reference_start": 21, "reference_end": 229, "reference_sequence": "MHSRSKFGIILKFNHFLWPGMRLEIENGLEFVCDPFLNERGKILFLHAFTGNITNKLSFRTHFKDYSFYGINFPGHGNSVIHNQSELDFNFWIKLVQQFFNKYQLKNVVLFGHSIGGGLAIALTQVLTKEQIKGIILEAPLNPGIRATPPSIISALVPDTNEDFEAVQRALIYNIEQRFGANFKDFCAKQKQKMIQKYAPLKVMLQPEQAEQRLQLIDAAFKRLSYPTLWIHGQEDGIVRYLPSKAYLESLHNPLIELVGLSNTAHTTFFEQPQQFLQLVEQFLNKLNK" }, "paralog": false, "identity": 0.9930795847750865, "score": 577.0, "evalue": 1.5e-211, "description": "Internal stop codon at 539947, 540583. Nonsense mutation around 539947, 540583.", "psc": { "uniref90_id": "UniRef90_P75333", "gene": "MenH", "product": "Putative esterase/lipase 1", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75333", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0016020", "GO:0052689" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75333", "EC:3.1.-.-", "UniRef:UniRef50_P75333", "COG:COG0596", "COG:HR", "GO:0016020", "GO:0052689" ] }, "pscc": { "uniref50_id": "UniRef50_P75333", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75333" ], "product": "Putative esterase/lipase 1" } }, "id": "GIHJHMOALN_970", "locus": "GCA009939745_00955" }, { "type": "cds", "sequence": "contig_1", "start": 540619, "stop": 540765, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCTCGAAGGCTTACCTGGAATCCTTGCACAATCCTTTAATTGAACTAGTGGGCTTATCCAACACGGCCCACACCACTTTCTTTGAGCAGCCCCAGCAGTTTCTCCAACTAGTGGAACAGTTTTTAAACAAGTTAAACAAATAA", "aa": "MPSKAYLESLHNPLIELVGLSNTAHTTFFEQPQQFLQLVEQFLNKLNK", "aa_hexdigest": "9aec1057b88e71a1af9acc00c25d6d9b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5558.321899999999, "isoelectric_point": 5.99926471710205 }, "id": "GIHJHMOALN_971", "locus": "GCA009939745_00956" }, { "type": "cds", "sequence": "contig_1", "start": 540826, "stop": 541443, "strand": "-", "frame": 1, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274", "KEGG:K02986", "RefSeq:WP_010874802.1", "SO:0001217", "UniParc:UPI000013500D", "UniRef:UniRef100_P46775", "UniRef:UniRef50_P46775", "UniRef:UniRef90_P46775" ], "nt": "ATGAAATACACTGGTAGTATTTTCAAACGATCACGCCGCTTAGGTTTTTCGTTACTAGAAAACAACAAGGAGTTTTCGAAAGGGAAGAAGCGGAAAACGATTCCTGGACAACACGGGAACCGTTTCCGTAGTTCCACTATGTCTGGTTATGCACAACAGTTGCAAGAAAAGCAACGGATGCAATACATGTATGGGATTACTGATAAACAGTTTAGACGCTTGTTTAGACTGGTATTAAAACAACGGGGTAACTTAGCGGTTAACCTGTTCCGTGTTTTAGAGTCACGTTTAGACAACATTGTGTACCGCATGGGTTTTGCTCCAACCCGTCGCAGTGCGCGCCAATTAGTTAACCACGGCCACGTATTACTCAACGATCGTACCGTGGACACCCCTTCCATTATTCTCAATCCAGGGGATAAGGTACGCTTGAAGGCCAAAACAATTAAGATTCCAATTGTTAAGGCGGCGAGCGAAAGCGGTGTGGTCTCCCCGTTTGTGGAAACCAACAACAAGACCTTTGAAGGGACATACGTGCGCTTCCCAGAACGTAGTGAACTCCCTGCTGGTATTAACGAATCGTACGTGGTCGAATGGTACAAGCGTTTGGTTAAGTAG", "aa": "MKYTGSIFKRSRRLGFSLLENNKEFSKGKKRKTIPGQHGNRFRSSTMSGYAQQLQEKQRMQYMYGITDKQFRRLFRLVLKQRGNLAVNLFRVLESRLDNIVYRMGFAPTRRSARQLVNHGHVLLNDRTVDTPSIILNPGDKVRLKAKTIKIPIVKAASESGVVSPFVETNNKTFEGTYVRFPERSELPAGINESYVVEWYKRLVK", "aa_hexdigest": "8a9fa98fa0470c7da55eccdbcc2f8093", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013500D", "ncbi_nrp_id": "WP_010874802.1", "uniref100_id": "UniRef100_P46775", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013500D", "RefSeq:WP_010874802.1", "UniRef:UniRef100_P46775" ] }, "ips": { "uniref100_id": "UniRef100_P46775", "uniref90_id": "UniRef90_P46775" }, "psc": { "uniref90_id": "UniRef90_P46775", "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_P46775", "cog_id": "COG0522", "cog_category": "J", "kegg_orthology_id": "K02986", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274" ] }, "pscc": { "uniref50_id": "UniRef50_P46775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P46775" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "GIHJHMOALN_972", "locus": "GCA009939745_00957" }, { "type": "cds", "sequence": "contig_1", "start": 541466, "stop": 542470, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAACCAAAGAGGCTTCATCAAATGTTGATGTGGTACAACAACCAGTCAAACCACTCATGCCAGTGATGGTAGAACAGCTCAGAACAACGGAGTTACAGCCAACTACTGAAATTAATCTGTTTGCCAACAGCGACATTAACAGCATTATTGCCGAGTTAAAGCAAGGTCGTTCTAACCCTGCCATTAACTTCGATGACATCTTTAAAATGTCAAGTTACCAAATGGTGGTCAAAAAGTCGTTTGTCCAAATTAGTGACTTTATTACTAATTCCAAAACCGACATTACCAACCGCTTCTTGTTAATCAAAAAGGAATTACAAGCCGAATTGACCCGCTTAATTGAAGAAAACGAACAGCTCAAAGCGGAGTTTTTAAACGCTAAAGACCTTTCGGTGTACCAAAAGGATGAACTGCTACGCTCTTTATCCAACGACTTTACGATTGCTCACCGTCCAAGTGACAGTTACGAACAATTGCAAAAGTCCGGTGAGTTAGTGCGCAACATTCAAAAAGCGATCTTGGAAAACGAAAGCAAGATTAAAAACATCCAGATTACGCTAAAAGAACTTAAAGCCGTTTATAAGCTCTGCAGTGATACTGTTTTAAACGGGATGGCGAAGCTAGACAGTGTGTTGCGCTTTAACAAGAAGGAAAAGGATCCTTTATTGTTAAACAGCATGGAAACATTGTCTAGCTTTGAGACTGAACCGCAAGCCATTATTGAAGATCTCTTGGACTTCTCCAGTAGCTTTGACAAGATGTCCAATGAACAATTGGATGAGTTTGTCTACCAAAACCTAGACAGTGGGTTAAACCTTGATTTAGATGGGTTTGATCATCAGTTAAGCAGCATGAACATCCACGGGTTAGAGCCATTAGATCCAATGAAGTTAGATGACTTTGATTTTGAAACTTTAACTCCAGATAAAACCTCTAACCTCTCTAGTATCTTGGATGATGAACTAATGGAAAACGGTGGGGATTTTAATCTTGATTATTAA", "aa": "METKEASSNVDVVQQPVKPLMPVMVEQLRTTELQPTTEINLFANSDINSIIAELKQGRSNPAINFDDIFKMSSYQMVVKKSFVQISDFITNSKTDITNRFLLIKKELQAELTRLIEENEQLKAEFLNAKDLSVYQKDELLRSLSNDFTIAHRPSDSYEQLQKSGELVRNIQKAILENESKIKNIQITLKELKAVYKLCSDTVLNGMAKLDSVLRFNKKEKDPLLLNSMETLSSFETEPQAIIEDLLDFSSSFDKMSNEQLDEFVYQNLDSGLNLDLDGFDHQLSSMNIHGLEPLDPMKLDDFDFETLTPDKTSNLSSILDDELMENGGDFNLDY", "aa_hexdigest": "0ff80898b9ddaa1a42b984d2a32d594b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38118.626200000086, "isoelectric_point": 4.484448814392089 }, "id": "GIHJHMOALN_973", "locus": "GCA009939745_00958" }, { "type": "cds", "sequence": "contig_1", "start": 542627, "stop": 544024, "strand": "-", "frame": 3, "gene": "hmw1", "product": "Cytadherence high molecular weight protein 1", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q50365", "UniRef:UniRef90_Q50365" ], "nt": "TTGGAAGCAGATGTACCAGCACCAGAAGTTGCTGAACCTAGCTATGAGGTACAACCAGAGGTAGCTGCTGAGCCTGTTTACGATGTTCAACCAGAAGTAGCTGTAGAACCGGTGGGAGAAACTACTGCAACAGTAGAACCACAAGCAGTAGAAATTCAACCAGAAGTAGTTGTAGAACCAATTGTTGAATCACAACTAGAACAACCTGTGGAAGTTCAAGCTGAAATGGTTCAACCAGAAGTAGCTGTAGAGCCACAATTAGAAGTAAGTCTAGATCCAATTGGAGAAACTGCACCAATATTGGAACAAGTTGAACCACAAGCTGTACAAACCCAACCAGAGATTCCAGCTGAACAATCAGCAGTAGAGCTTCAACCAGAACCAGTTACAGAAGTTCAATCTGAAATGGTTCAACCAGAAGCAGCTGCAGAGCCAGTTACAGAAGCACAACAAACTGAACCAACACCAGTTGTTGAAACTATTGCAGAGATTACACCTCAGGTAGTTACAGAGCCTGTAGTAGCTGTTGTAGAACATCAACCGGAAGCTGTTGCCGAGCCACTTCCAGTAGAACCAGCTGTGGCAGGTGTGTCTGAATTAATTCCAACGGAACAAGTTCAACCAGAAGTGGTTGTGGAATCGACTCCAGTTGCAGAAGTTCAATCTGAAATGGTTCAACCAGAAGTAGCTGTAGAACCGATTGTTGAACCACAACCAGAACAACCTGTGGAAGTCCAACCAGAAGTAATTACAACCCCGGAAGTAGCTTCAGTTTTAGAAGTTCAACCTGAAAATCCTGTTGTAGAAGTGGAACAAGTAGTTGAACCACAACCTGAAACCCCTGTAGAAGTTCAACCAGAACCAGTTGTTGAAACTGTTCAAGAAGCTGTGGCTGAACCAACCCCAGTAGTTGAACCACAACCACAAGCTGCACCTCAACCAGCGGTTTATGAATGGAATCTAACTCCAGAAGCTGCTCCTGTTGAACAACCAGAAGTAATTCCAGTAACAGTAGTAGAAAGCCAAGCTACCGCTACCGCAGAACCTCAACCAGCAGTAGCACCCGTTGCTGATATGGATTATGTCTTACATCTCACCGATACAGTAAAAAACCAACCACAGACTGCACCAGTACAACCAACTACCCCAATTAAAATTGAAGTGGCGGAATCAACTCCTACCGTTACAACCAGTCCAGTTGAACCAACAATTGCCCCACCACTGTTTGAAATTGAACTCAATAATACAACTTCATCTGACTTACCATTAGTTGAGGTTGTGGACTTTAAGCACAATCAACACGGTGCTGTCGGTACCCACAGCTTTGATGATTTTACCCCACCAGAAGTGGGTATGGAAAGCAAGACCCACTGTCACAGTAACAGTGAGGTTGTATGA", "aa": "MEADVPAPEVAEPSYEVQPEVAAEPVYDVQPEVAVEPVGETTATVEPQAVEIQPEVVVEPIVESQLEQPVEVQAEMVQPEVAVEPQLEVSLDPIGETAPILEQVEPQAVQTQPEIPAEQSAVELQPEPVTEVQSEMVQPEAAAEPVTEAQQTEPTPVVETIAEITPQVVTEPVVAVVEHQPEAVAEPLPVEPAVAGVSELIPTEQVQPEVVVESTPVAEVQSEMVQPEVAVEPIVEPQPEQPVEVQPEVITTPEVASVLEVQPENPVVEVEQVVEPQPETPVEVQPEPVVETVQEAVAEPTPVVEPQPQAAPQPAVYEWNLTPEAAPVEQPEVIPVTVVESQATATAEPQPAVAPVADMDYVLHLTDTVKNQPQTAPVQPTTPIKIEVAESTPTVTTSPVEPTIAPPLFEIELNNTTSSDLPLVEVVDFKHNQHGAVGTHSFDDFTPPEVGMESKTHCHSNSEVV", "aa_hexdigest": "309400ab5ee078d4cbf6202b37500909", "start_type": "TTG", "rbs_motif": null, "genes": [ "hmw1" ], "truncated": "both", "pseudogene": { "start": 542324, "stop": 544324, "observations": { "mutation": [ 542629, 544147 ], "stop": [ 542629, 544147 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50365", "query_cov": 1.0, "subject_cov": 0.4567779960707269, "identity": 0.946, "score": 437.0, "evalue": 2.19e-140, "gene_start": 1, "gene_end": 465, "reference_start": 167, "reference_end": 631, "reference_sequence": "MKKSKEAVFEDKDYTEENPEQIFGNLYDGKLTVQDGKVKIAYDGDGNGYYIAFNSETGVYYDPYGDTEYDISVLFDANGNSFVFADAPTVEVLAGEQEQTEAEPDYLQYVGNEAYGYYDEAGEWVWSGYFEGDQWISTLPQTEAEEKQFGFEDNIETTPTASEDFGLEADVPAPEVAEPSYEVQPEVAAEPVYDVQPEVAVEPVGETTATVEPQAVEIQPEVVVEPIVESQLEQPVEVQAEMVQPEVAVEPQLEVSLDPIGETAPILEQVEPQAVQTQPEIPAEQSAVELQPEPVAEVQSEMVQPEAAAEPVTEAQQTEPTPVVETIAEITPQVVTEPVVAVVEHQPEAVAEPLPVEPAVAGVSELIPTEQVQPEVVVESTPVAEVQSEMVQPEVAVEPIVEPQPEQPVEVQPEVITTPEVASVLEVQPENPVVEVEQVVEPQPETPVEVQPEPVVETVQEAVAEPTQVVEPQPQAAPQPAVYEWNLTPEAAPVEQPEVIPVTVVESQATATAEPQPAVAPVADMDYVLHLTDTVKNQPQTAPVQPTTPIKIEVAESTPTVTTSPVEPTIAPPLFEIELNNTTSSDLPLVEVVDFKHNQHGAVGTHSFDDFTPPEVGMESKTHCHSNSEVVWRVSEPKTVPVPPAVSSINIQTVNRVVEPTISTPTTPVVESAPAIEIFVDTPPVETKEASSNVDVVQQPVKPLMPVMVEQLRTTELQPTTEINLFANSDINSIIAELKQGRSNPAINFDDIFKMSSYQMVVKKSFVQISDFITNSKTDITNRFLLIKKELQAELTRLIEENEQLKAEFLNAKDLSVYQKDELLRSLSNDFTIAHRPSDSYEQLQKSGELVRNIQKAILENESKIKNIQITLKELKAVYKLCSDTVLNGMAKLDSVLRFNKKEKDPLLLNSMETLSSFETEPQAIIEDLLDFSSSFDKMSNEQLDEFVYQNLDSGLNLDLDGFDHQLSSMNIHGLEPLDPMKLDDFDFETLTPDKTSNLSSILDDELMENGGDFNLDY" }, "paralog": false, "identity": 0.9594594594594594, "score": 854.0, "evalue": 1.86e-305, "description": "Internal stop codon at 542629, 544147. Nonsense mutation around 542629, 544147.", "psc": { "uniref90_id": "UniRef90_Q50365", "gene": "hmw1", "product": "Cytadherence high molecular weight protein 1", "uniref50_id": "UniRef50_Q50365", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50365", "UniRef:UniRef50_Q50365", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q50365", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50365" ], "product": "Cytadherence high molecular weight protein 1" } }, "id": "GIHJHMOALN_974", "locus": "GCA009939745_00959" }, { "type": "cds", "sequence": "contig_1", "start": 544145, "stop": 544522, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16713.10" ], "nt": "ATGAAGAAAAGCAAAGAAGCAGTTTTTGAAGACAAGGATTACACTGAGGAAAATCCCGAACAGATCTTTGGTAATTTGTACGATGGTAAATTAACTGTTCAAGATGGCAAAGTCAAGATTGCCTATGACGGTGATGGCAATGGTTATTACATTGCCTTTAACAGTGAAACTGAAGTTTACTATGATCCTTATGGTGACACGGAATACGACATTAGCGTGTTGTTTGACGCCAATGGTAATCCGTTCGTGTTTGCTGATGCACCAACAGTGGAAGTTTTAGCGGGGGAACAGGAACAAACCGAAGCAGAACCGGATTACCTTCAGTATGTCGGTAATGAGGCTTATGGTTACTATGATGAAGCTGGGGAGTGGGTTTGA", "aa": "MKKSKEAVFEDKDYTEENPEQIFGNLYDGKLTVQDGKVKIAYDGDGNGYYIAFNSETEVYYDPYGDTEYDISVLFDANGNPFVFADAPTVEVLAGEQEQTEAEPDYLQYVGNEAYGYYDEAGEWV", "aa_hexdigest": "7175e18e03056a3fd19b3fec8aa18cff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16713.10", "name": "EAGR_box", "length": 22, "aa_cov": 0.176, "hmm_cov": 1.0, "evalue": 9.485989948792235e-10, "score": 33.555137634277344, "start": 104, "stop": 125 } ], "seq_stats": { "molecular_weight": 14159.973599999998, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_975", "locus": "GCA009939745_00960" }, { "type": "cds", "sequence": "contig_1", "start": 544586, "stop": 544867, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTAAACCTCTACATCTTAATGATGGCCTTACTAATGACCTTAGTGGTATCATTGTTAATTGGCACTATAGCCTCGATTGAATACCTCGTCTTTTTAAGTGGTAAACCAAGTAAGAACTTCGTTAAGTATGGTAGCTATTTCTTTTTAATGCCAAGGGTCTTGGTACAAGCGTTGTTAATGCCCTTGTACTTGGCCTTGTTTAAGCCCTTAATTAGGATTGCTGAAAATAATTTAAGAAATTATTTAAGGGTATACAACCTCTCATGGAAAAGATAA", "aa": "MILNLYILMMALLMTLVVSLLIGTIASIEYLVFLSGKPSKNFVKYGSYFFLMPRVLVQALLMPLYLALFKPLIRIAENNLRNYLRVYNLSWKR", "aa_hexdigest": "74f4eb010f34818aa31f324444d287d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10864.250999999987, "isoelectric_point": 10.136186790466308 }, "id": "GIHJHMOALN_976", "locus": "GCA009939745_00961" }, { "type": "cds", "sequence": "contig_1", "start": 544898, "stop": 545041, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACCTGACTGATGGCAATTTTCAAGCTGCTGGTAACCAGAGCTTTGGCCGGGGCTTTGATGTCAATGTCCACATCCTACAAGGCATTACGATGGCAGCCTTTATTAGCTTTTTTGTGGGGTTAGAAATCTTTTTGGGTTGA", "aa": "MYLTDGNFQAAGNQSFGRGFDVNVHILQGITMAAFISFFVGLEIFLG", "aa_hexdigest": "bb18af03e57aff4bd5780654a1f755b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5115.793199999997, "isoelectric_point": 4.535092353820801 }, "id": "GIHJHMOALN_977", "locus": "GCA009939745_00962" }, { "type": "cds", "sequence": "contig_1", "start": 545111, "stop": 545377, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACTAAGCTTTATCTTTAATATTTTTTCCATTAATGTCACTAGTGTGCTCAAGGTGAGTTTCACAAGAATTCCCTTTGCCTTAATTGGTTGGATGTTTGGACCGGTGTGGGGCTTTACCTTTGGCGCCATTGCCGACACGATGGACTGGCTTACTAGGGGCTATACCTGGTTTTGGTTGTTTGCGATTCAAAAACCCATGTTCTGCTTTTTAGCGGGGCTGGTTAAGGGCGTTTATCAGGTACGCCAAAGTAGTACTAACTGA", "aa": "MALSFIFNIFSINVTSVLKVSFTRIPFALIGWMFGPVWGFTFGAIADTMDWLTRGYTWFWLFAIQKPMFCFLAGLVKGVYQVRQSSTN", "aa_hexdigest": "f28142e6e63ca6ca4e1b4291e79cfbc1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10104.854499999996, "isoelectric_point": 9.860197639465333 }, "id": "GIHJHMOALN_978", "locus": "GCA009939745_00963" }, { "type": "cds", "sequence": "contig_1", "start": 545494, "stop": 546219, "strand": "-", "frame": 1, "gene": null, "product": "MPN449 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363", "UniRef:UniRef90_Q50363" ], "nt": "ATGTTGTTAATTGGAGCGGCCGCCATTGCTTACCTCGTGGTGGTAAACCTCTTGTTTTACAGGTATGTAAACCCTGACAGTAACCTAACTAAAGCGGTTACTGGTCAGAATAATACCGACAGGCCAGCGTTAGTTGATTTAAACGGTGGCATTAACCTCTTTTTCCCTGTTAGTGCTTCTACTTTAATTACCTTGATCTTCTTGGGTTTTGGTAGTACTTCCTTCCTTTTAGCCTTTCAAGGTAAGCCCTACAGTTTTGCTACTAGAAGTCAAACCTTTAAGGCAATGCACTTTCTCAAGCACCAGTTTGGGGTAACTGATTTTCCCAGGATCAATGACAACTACCGGTATAAGGTCAGAATTAAGTGGATCTTCTGGACAATCTTCTTGTTTGTGGCACTCAATGCCCTACCTGGGGGTAACATTATTACAGGTGGTATTTTAAACCCCTCTTTTCTGTTAAATGCCTTAAGGTCTAATGAATTAAAAATTAACAGTGAGACCTTTAAAACTATCTTTGTTGGTTTTTCCATATTTTACCTAGCGAGCATTATTCCCTTTGCCCTCATTAGTATTATTGCCTTTGTTTTGAGTCCTAAACCAAGTTCGGATCAAACAAACGAAGTGTTGAACCGTTACGTGCAAGAGGAAATGCAACAGCCATTTAAAACGGATTGTGATCCTAACAACGACAATGATTTAACACCCCCAGCTGTTTTTGGGTAA", "aa": "MLLIGAAAIAYLVVVNLLFYRYVNPDSNLTKAVTGQNNTDRPALVDLNGGINLFFPVSASTLITLIFLGFGSTSFLLAFQGKPYSFATRSQTFKAMHFLKHQFGVTDFPRINDNYRYKVRIKWIFWTIFLFVALNALPGGNIITGGILNPSFLLNALRSNELKINSETFKTIFVGFSIFYLASIIPFALISIIAFVLSPKPSSDQTNEVLNRYVQEEMQQPFKTDCDPNNDNDLTPPAVFG", "aa_hexdigest": "6d75d32db0a653f77671fa6947da4dca", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 545494, "stop": 546519, "observations": { "mutation": [ 546246, 546255 ], "stop": [ 546246, 546255 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50363", "query_cov": 1.0, "subject_cov": 0.5379464285714286, "identity": 1.0, "score": 472.0, "evalue": 4.01e-165, "gene_start": 1, "gene_end": 241, "reference_start": 208, "reference_end": 448, "reference_sequence": "MTFSDLLTKLQDNLDIVFNANALKERIQSDPAFAKTIREQLKLLYFLEQKQAKAKTKKKDFQPVFQNLEARFVSLGQTKLANTELNLKLDLTDATDLANYLPIAVCNLFNRDLNSFSKLSSVQPTAVEKTTNGANKPTVTIDLNQPRIHTTGTVSPELENFVNESLEARARQRAFMRVTSERMVGKIFEFQFKSQWIKWAQLAIFISMLLIGAAAIAYLVVVNLLFYRYVNPDSNLTKAVTGQNNTDRPALVDLNGGINLFFPVSASTLITLIFLGFGSTSFLLAFQGKPYSFATRSQTFKAMHFLKHQFGVTDFPRINDNYRYKVRIKWIFWTIFLFVALNALPGGNIITGGILNPSFLLNALRSNELKINSETFKTIFVGFSIFYLASIIPFALISIIAFVLSPKPSSDQTNEVLNRYVQEEMQQPFKTDCDPNNDNDLTPPAVFG" }, "paralog": false, "identity": 0.9941348973607038, "score": 667.0, "evalue": 4.43e-244, "description": "Internal stop codon at 546246, 546255. Nonsense mutation around 546246, 546255.", "psc": { "uniref90_id": "UniRef90_Q50363", "product": "MPN449 family protein", "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50363", "UniRef:UniRef50_Q50363" ] }, "pscc": { "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363" ], "product": "Uncharacterized protein MG314 homolog" } }, "id": "GIHJHMOALN_979", "locus": "GCA009939745_00964" }, { "type": "cds", "sequence": "contig_1", "start": 546253, "stop": 546840, "strand": "-", "frame": 1, "gene": null, "product": "MPN449 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363", "UniRef:UniRef90_Q50363" ], "nt": "ATGACTTTTAGCGATCTTTTAACCAAGTTACAAGACAATTTAGACATTGTTTTTAATGCCAATGCCCTCAAAGAGCGGATCCAAAGCGACCCGGCGTTTGCTAAAACAATTCGCGAACAGTTAAAGCTGTTGTACTTTTTAGAGCAAAAGCAAGCTAAGGCTAAAACCAAGAAAAAGGACTTTCAACCTGTTTTTCAAAACTTAGAAGCGCGCTTTGTTAGTTTAGGGCAAACCAAACTAGCGAACACCGAACTTAACCTCAAACTTGATTTAACTGATGCCACGGACTTGGCTAACTATTTACCAATTGCCGTGTGCAATCTCTTTAACCGTGATTTAAATAGCTTTAGTAAGTTAAGTAGCGTTCAACCAACAGCTGTTGAAAAAACAACCAATGGAGCTAATAAACCGACGGTTACAATTGATTTAAACCAACCACGGATACATACAACTGGTACTGTAAGCCCAGAGTTGGAAAACTTTGTCAACGAATCACTCGAAGCACGTGCACGCCAACGTGCCTTTATGCGGGTCACCTCGGAACGGATGGTAGGCAAAATCTTTGAATTCCAGTTTAAAAGCCAGTGA", "aa": "MTFSDLLTKLQDNLDIVFNANALKERIQSDPAFAKTIREQLKLLYFLEQKQAKAKTKKKDFQPVFQNLEARFVSLGQTKLANTELNLKLDLTDATDLANYLPIAVCNLFNRDLNSFSKLSSVQPTAVEKTTNGANKPTVTIDLNQPRIHTTGTVSPELENFVNESLEARARQRAFMRVTSERMVGKIFEFQFKSQ", "aa_hexdigest": "d7f164fbd3bcdda1858d2b919b7ec8c9", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 545950, "stop": 546840, "observations": { "mutation": [ 546246, 546255 ], "stop": [ 546246, 546255 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50363", "query_cov": 1.0, "subject_cov": 0.43526785714285715, "identity": 1.0, "score": 369.0, "evalue": 4.35e-125, "gene_start": 1, "gene_end": 195, "reference_start": 1, "reference_end": 195, "reference_sequence": "MTFSDLLTKLQDNLDIVFNANALKERIQSDPAFAKTIREQLKLLYFLEQKQAKAKTKKKDFQPVFQNLEARFVSLGQTKLANTELNLKLDLTDATDLANYLPIAVCNLFNRDLNSFSKLSSVQPTAVEKTTNGANKPTVTIDLNQPRIHTTGTVSPELENFVNESLEARARQRAFMRVTSERMVGKIFEFQFKSQWIKWAQLAIFISMLLIGAAAIAYLVVVNLLFYRYVNPDSNLTKAVTGQNNTDRPALVDLNGGINLFFPVSASTLITLIFLGFGSTSFLLAFQGKPYSFATRSQTFKAMHFLKHQFGVTDFPRINDNYRYKVRIKWIFWTIFLFVALNALPGGNIITGGILNPSFLLNALRSNELKINSETFKTIFVGFSIFYLASIIPFALISIIAFVLSPKPSSDQTNEVLNRYVQEEMQQPFKTDCDPNNDNDLTPPAVFG" }, "paralog": false, "identity": 0.9932432432432432, "score": 565.0, "evalue": 1.5e-204, "description": "Internal stop codon at 546246, 546255. Nonsense mutation around 546246, 546255.", "psc": { "uniref90_id": "UniRef90_Q50363", "product": "MPN449 family protein", "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50363", "UniRef:UniRef50_Q50363" ] }, "pscc": { "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363" ], "product": "Uncharacterized protein MG314 homolog" } }, "id": "GIHJHMOALN_980", "locus": "GCA009939745_00965" }, { "type": "cds", "sequence": "contig_1", "start": 547029, "stop": 547751, "strand": "-", "frame": 2, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360", "SO:0001217", "UniRef:UniRef50_Q50362", "UniRef:UniRef90_Q50362" ], "nt": "ATGACCGTAGTTTATGGCGCCGATATTGGGTTAATTCACCAGCAGTTAAACCAACTGCTCAACCCAGCCGCTTGTAAACAGGTCTGGTTTCAAGATGTTAATAAGCTGTATGATGTGGTGTTAAACCAGAATCTGTTTGCTGAGGACACTAAACCGATCCTCATTCACAACTGCAGTTTTTTGGAAAAGAACAACTTAACTAAGGCTGAATTACACTGCTTAAAAACACTTAAAGATACTGATGTGGTGGTCACCATTTACAGCGACAGTCCCGCTAATGCCCTCATTAATGACCGGGCAATTACCAAGTATGCTTGTAAACCAGTCACAGCTAAAACCATCCACCAGGTGATCAGTAAGGCCGCTAAGACACTCAAACTCAACTTAAACCCTGATTTAATTGACCACTTAGCCACGATTCTACCCTTTAACTTAGGGGTCATTGAACAGGAGTTGCGTAAGTTAACTCTACTCAGCCCGGCAGAATTACAGGACAAAAAAATGTTAGAAGCCGTTTTGTGTGATTACCAAACCAGTCAAATCTTACAATTAACTGACGCCATGGTGCGTTTGCAAACAGCTAAAGCCTTAAAGTTGATTGAACGCTTGTTTTTACCAAAGCAGCTCACACCACCGCAATTCCTAGAATTTTTAGCAAATGAACTACTTTTGGCCTTGATGGTCAAAGGCGTTAAACCACAAGCTGTTTTCCAACTACAGTGA", "aa": "MTVVYGADIGLIHQQLNQLLNPAACKQVWFQDVNKLYDVVLNQNLFAEDTKPILIHNCSFLEKNNLTKAELHCLKTLKDTDVVVTIYSDSPANALINDRAITKYACKPVTAKTIHQVISKAAKTLKLNLNPDLIDHLATILPFNLGVIEQELRKLTLLSPAELQDKKMLEAVLCDYQTSQILQLTDAMVRLQTAKALKLIERLFLPKQLTPPQFLEFLANELLLALMVKGVKPQAVFQLQ", "aa_hexdigest": "086fc53dabb39ca3579aac98dda307f0", "start_type": "ATG", "rbs_motif": null, "genes": [ "holA" ], "truncated": "5-prime", "pseudogene": { "start": 546852, "stop": 547796, "observations": { "mutation": [ 546980, 547031 ], "stop": [ 546980, 547031 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50362", "query_cov": 1.0, "subject_cov": 0.7643312101910829, "identity": 1.0, "score": 462.0, "evalue": 4.44e-163, "gene_start": 1, "gene_end": 240, "reference_start": 16, "reference_end": 255, "reference_sequence": "MGCGACCILWVQSFTMTVVYGADIGLIHQQLNQLLNPAACKQVWFQDVNKLYDVVLNQNLFAEDTKPILIHNCSFLEKNNLTKAELHCLKTLKDTDVVVTIYSDSPANALINDRAITKYACKPVTAKTIHQVISKAAKTLKLNLNPDLIDHLATILPFNLGVIEQELRKLTLLSPAELQDKKMLEAVLCDYQTSQILQLTDAMVRLQTAKALKLIERLFLPKQLTPPQFLEFLANELLLALMVKGVKPQAVFQLQWNVNPFRLKAIQSQYRFWSTNQLTALINAIWQLDIKIKRNDGLAIHLLKHFVLRFFAQK" }, "paralog": false, "identity": 0.9936305732484076, "score": 615.0, "evalue": 1.06e-225, "description": "Internal stop codon at 546980, 547031. Nonsense mutation around 546980, 547031.", "psc": { "uniref90_id": "UniRef90_Q50362", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q50362", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50362", "EC:2.7.7.7", "UniRef:UniRef50_Q50362", "COG:COG1466", "COG:L", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_Q50362", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50362" ], "product": "Probable DNA polymerase III subunit delta" } }, "id": "GIHJHMOALN_981", "locus": "GCA009939745_00966" }, { "type": "cds", "sequence": "contig_1", "start": 547939, "stop": 548073, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACTTCCACCTTAATCCTCATTGTGGTCAGTCCGATTACTTTGCACTTAAATCGAAAATTAAACGCTTTAAGTGTGTTTCATAACCTCTTGTTTACTCCCATAGCCTTGTTTTACTTCTGTGCTAGCTGA", "aa": "MLTSTLILIVVSPITLHLNRKLNALSVFHNLLFTPIALFYFCAS", "aa_hexdigest": "c80a687094b032b51a396136492493e0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4946.955299999999, "isoelectric_point": 9.304415702819824 }, "id": "GIHJHMOALN_982", "locus": "GCA009939745_00967" }, { "type": "cds", "sequence": "contig_1", "start": 548089, "stop": 548409, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAGTGGTTTTCACCTTAGTTTTTTGTTTGGTGTCATGGAGCGCTGGTTGTACAAGCGCTTTTACATCAATAAAATAACGGGTTTTGTAATGCTGTTGCTGTACCTCTTTTTGGTCGGATTTGCCTTTTCAGCTTTACGGGTTTTTGTCTCAAAGCTGTTACGGCAAATGTTTCCCAAGCAACTACCCGAAAATAATTTGGGCTTAACGGCGCTGTTAATCATCTTAATGAGTCCGGGAGCGTTACACAACTTTGGCTTTAACTTTTCCTTTTTAGCGTGCTTTGTGTTGTTTGCCATTAATAAGGTGAAGCTGTGA", "aa": "MISGFHLSFLFGVMERWLYKRFYINKITGFVMLLLYLFLVGFAFSALRVFVSKLLRQMFPKQLPENNLGLTALLIILMSPGALHNFGFNFSFLACFVLFAINKVKL", "aa_hexdigest": "0a5e240b53b4d9b5b8e822d756a33c53", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12265.821599999981, "isoelectric_point": 10.294843482971189 }, "id": "GIHJHMOALN_983", "locus": "GCA009939745_00968" }, { "type": "cds", "sequence": "contig_1", "start": 548551, "stop": 548685, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATAGTTAACCTAATTAGTTTAACGGTACACTGGAAGTTTGGCTTACTGTTAATCAGTTGTAGTGCCGCTTTACTAGCATACTGTCAGTTAATGCCAGAGTTTCAACTAGCCAAACTAATGAAAGCGAACTGA", "aa": "MIVNLISLTVHWKFGLLLISCSAALLAYCQLMPEFQLAKLMKAN", "aa_hexdigest": "ac259c2c66f82dcac581e709e9b4dad9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4938.056199999999, "isoelectric_point": 8.803689765930176 }, "id": "GIHJHMOALN_984", "locus": "GCA009939745_00969" }, { "type": "cds", "sequence": "contig_1", "start": 548824, "stop": 549090, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGTAAACTACCGCAACCCAGCGAGTTTGAACTTCGACAGCCTTAACAGCTTTGGCGCTTACCGCAGTCCAAGTAGCTTTTACCCACTTAGAAGACCATTGGAATTGTCGAGCTTAAGGCGTAACCGCAGTTCCTTTTTCAACACGCACCGCTTTGATTTAGGGAGCAATTACACCAGTTTCACCCCGCGTTACCGCTCACCGTTACGTGGTGGTTTGTCTCAACGCTTTCCGCTCCGTTCCAGCTGGAGTAAAGAGTTTTAA", "aa": "MAVNYRNPASLNFDSLNSFGAYRSPSSFYPLRRPLELSSLRRNRSSFFNTHRFDLGSNYTSFTPRYRSPLRGGLSQRFPLRSSWSKEF", "aa_hexdigest": "b4490d7ee4ccee62d3db0bd631e5022c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10322.40539999999, "isoelectric_point": 11.510265922546388 }, "id": "GIHJHMOALN_985", "locus": "GCA009939745_00970" }, { "type": "cds", "sequence": "contig_1", "start": 549157, "stop": 549393, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAACCACAACCGTGTCAGTCAACTTGTGTTAATAATGAAAGTGGTGCAATTACCATTCACACCACCAACCGTTCCTTATTGTTGGAAAAGCTAGCGAGTTTGGGTCACCTCCACGATGCTTCTACCCGTACGCCTTTACCGCACGAGCGCTACCAGTTAGCTCCACCGAGTGAATATGTGGCTACCAAGTACAACGAACCGCTGTTTAACCTTCCAGCAATCCGCAACAGTTGA", "aa": "MEPQPCQSTCVNNESGAITIHTTNRSLLLEKLASLGHLHDASTRTPLPHERYQLAPPSEYVATKYNEPLFNLPAIRNS", "aa_hexdigest": "0ffd67c7474e9ae00ab90b044496e46d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8684.680499999995, "isoelectric_point": 6.386338233947753 }, "id": "GIHJHMOALN_986", "locus": "GCA009939745_00971" }, { "type": "cds", "sequence": "contig_1", "start": 549415, "stop": 550842, "strand": "-", "frame": 1, "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q50360", "UniRef:UniRef90_Q50360" ], "nt": "ATGACAGATAAAGAAAGAGCAAAGCTAGCGAAAGCGTATGGTAAGCTAGCACAAAAGATCCAAAAGAGCTATCCCGACATTAATGTGGTTTATGGTCGTGATGCTAAAAATAAGCTTCACGCCCTGTACCAGGATCCTGAAACCGGGAACATCTTTTCTTTGGAAAAAAGAAAACAGCTCCCAGCGGATTATCCTCTGTTCGAATTGGACAGTGATGAACCAATTAGCTTTGCCCCCAAGATTATTCCACTCACTGCCTTTGATGGCAACAACAATGAGGTAATAGTCCAATACGACCAGGTCAACAATACCTTTTACGACCAAGATGGCAATGTTTTGGATGTGAGTGGTTACCGTGATGGGGAAAACATTCCCCTAGTGGACTATTTAAACTATGGTGGTAGTACCGCAAGTGCAGATACCACTACAAGCGAACCATTGAGTGGTGAGGGCTATCCGGACATTGATGCTGGTTTACCAGTTGTGGATCCCGATGCTACACCCGAACAGCAAGCTGATCAACTGTTCGGACTCGATCCACTACCTCAAGCACCAGACGAATATCAGGATACTACAGCACCACCAGCTTATGATCAGACCTTTGATCAAGCAACATACGACCAGCAAGCGTATGACCAAAACTATGATCCGAACGCTTACTATGACCAGCAAGCTTACGATCAGTCCTTTGATCAACAAGCGTATGATCAAGCCTATGATGCTAATGCTTACAATACACAGAACTATGATCAAGCTCACGATCCGAACGCTTACTATGATTCCCAAGCTTACAGCGATCCTGATCAAGCTAGTGCTGTAGCACCAATAGAGGTAGCACCACTCCAACCAGAACCAGTTGCACCAGTAGTGGAGCCAACTGCTGTTCCTATTGTTGAATCTGCTCCAATAGTAGAGGTTACTCCAACAGTAGAACCTACCCCAACACCAGTGGTAGAGACCGCACCAGTAGTGGAAGCTCCTAAGGTAGTGGAACCTACCCCAACACCAGTGGTAGAAGCTACTCCTGCCCCTAAAGTAGAGCCTAAGGTAGTGGAACAACCACAACCAACACCTGTAACTGTTGAGGTTGACTCACCTAAAGTTGAAATACCGAAGGTAGTAACAGCAAAAGTTGCACTCCAAGTGGCCCAACCAACCCCTGTTCCAGCTGTACCTAAAGTAGCACCACAACCAACCCCTGCTCCAGTAGTTGTCCAACCAACTGCTGTAGTTCAACCAGTTGTTAAGGCAGAACCGAAGGTAGTAACACCAACGCCAGCACCACAGGTAGTAGTTACTCCTCAAGTAGCTACACCTAAAGTGACACCTAAGGTAGTCCAAACAACTCCTGCGGTTCCACCAGTTGTAGTTCAACCAGAAGTGGTAGTCCAACCAATTATCCGTCCAACACAACCCGAACCGGAATGA", "aa": "MTDKERAKLAKAYGKLAQKIQKSYPDINVVYGRDAKNKLHALYQDPETGNIFSLEKRKQLPADYPLFELDSDEPISFAPKIIPLTAFDGNNNEVIVQYDQVNNTFYDQDGNVLDVSGYRDGENIPLVDYLNYGGSTASADTTTSEPLSGEGYPDIDAGLPVVDPDATPEQQADQLFGLDPLPQAPDEYQDTTAPPAYDQTFDQATYDQQAYDQNYDPNAYYDQQAYDQSFDQQAYDQAYDANAYNTQNYDQAHDPNAYYDSQAYSDPDQASAVAPIEVAPLQPEPVAPVVEPTAVPIVESAPIVEVTPTVEPTPTPVVETAPVVEAPKVVEPTPTPVVEATPAPKVEPKVVEQPQPTPVTVEVDSPKVEIPKVVTAKVALQVAQPTPVPAVPKVAPQPTPAPVVVQPTAVVQPVVKAEPKVVTPTPAPQVVVTPQVATPKVTPKVVQTTPAVPPVVVQPEVVVQPIIRPTQPEPE", "aa_hexdigest": "70100edd875ae52fafe40f6298207f4f", "start_type": "ATG", "rbs_motif": null, "genes": [ "hmw3" ], "truncated": "5-prime", "pseudogene": { "start": 549112, "stop": 550842, "observations": { "mutation": [ 549159, 549417 ], "stop": [ 549159, 549417 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50360", "query_cov": 0.9136842105263158, "subject_cov": 0.6439169139465876, "identity": 0.975, "score": 551.0, "evalue": 5.62e-189, "gene_start": 1, "gene_end": 432, "reference_start": 1, "reference_end": 434, "reference_sequence": "MTDKERAKLAKAYGKLAQKIQKSYPDINVVYGRDAKNKLHALYQDPETGNIFSLEKRKQLPADYPLFELDSDEPISFAPKIIPLTAFDGNNNEVIVQYDQVNNTFYDQDGNVLDVSGYRDGENIPLVDYLNYGGSTASADTTTSEPLSGEGYPDIDAGLPVVDPDATPEQQADQLFGLDPLPQAPDEYQDTTAPPAYDQTFDQATYDQQAYDQNYDPNAYYDQQAYDQSFDQQAYDQAYDANAYNTQNYDQAHDPNAYYDSQAYSDPDQAXXSAVAPIEVAPLQPEPVAPVVEPTAVPIVESAPIVEVTPTVEPTPTPVVETAPVVEAPKVVEPTPTPVVEATPAPKVEPKVVEQPQPTPVTVEVDSPKVEIPKVVTAKVALQVAQPTPVPAVPKVAPQPTPAPVVVQPTAVVQPVVKAEPKVVTPTPAPQVVVTPQVATPKVTPKVVQTTPAVPPVVVQPEVVVQPIIRPTQPEPEWKPSPASVVEPQPCQSTCVNNESGAITIHTTNRSLLLEKLASLGHLHDASTRTPLPHERYQLAPPSEYVATKYNEPLFNLPAIRNSWARFTRPTVESTPIASRFTGVTPMAVNYRNPASLNFDSLNSFGAYRSPSSFYPLRRPLELSSLRRNRSSFFNTHRFDLGSNYTSFTPRYRSPLRGGLSQRFPLRSSWSKEF" }, "paralog": false, "identity": 0.9792387543252595, "score": 773.0, "evalue": 1.87e-278, "description": "Internal stop codon at 549159, 549417. Nonsense mutation around 549159, 549417.", "psc": { "uniref90_id": "UniRef90_Q50360", "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "uniref50_id": "UniRef50_Q50360", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50360", "UniRef:UniRef50_Q50360", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q50360", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50360" ], "product": "Cytadherence high molecular weight protein 3" } }, "id": "GIHJHMOALN_987", "locus": "GCA009939745_00972" }, { "type": "cds", "sequence": "contig_1", "start": 550856, "stop": 551632, "strand": "-", "frame": 3, "gene": "p30", "product": "P30 adhesin", "db_xrefs": [ "GO:0005886", "GO:0007155", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniParc:UPI0002EA0F8A", "UniRef:UniRef100_A0AB38W863", "UniRef:UniRef50_P75330", "UniRef:UniRef90_P75330" ], "nt": "ATGCTAGTGCTGTTCAGCGCTTTAATAGTGCTTGCAACCTTAATTTTGGTACAGCACAACAATACCGAACTGACAGAAGTTAAGAGTGAATTGAGTCCCCTTAACGTTGTTTTACACGCAGAAGAGGATACAGTACAAATTCAGGGCAAGCCGATTACTGAGCAAGCATGGTTTATTCCTACAGTTGCTGGTTGCTTTGGTTTTAGTGCCCTAGCCATCATCTTGGGCCTTGCTATAGGACTGCCAATTGTGAAGCGCAAGGAAAAACGCTTATTGGAGGAAAAGGAACGCCAAGAACAGTTAGCGGAACAGCTACAACGCATTTCTGCCCAACAAGAAGAGCAACAAGCGTTAGAACAACAAGCAGCTGCTGAAGCCCATGCTGAAGCGGAAGTTGAACCAGCACCACAACCAGTACCAGTACCACCTCAACCCCAAGTCCAAATTAACTTCGGTCCCCGTACTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCAGGTATGCCGCCACACCCCGGTATGGCTCCAAGATCTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCAGGTATGCCGCCACACCCCGGTATGGCTCCAAGACCTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCAGGTATGCCGCCACACCCCGGTATGGCTCCAAGACCTGGTTTCCCACCTCAACCCGGTATGGCGCCTCGTCCCGGAATGCAACCACCACGTCCTGGCATGCCACCCCAACCCGGTTTTCCACCAAAACGCTAA", "aa": "MLVLFSALIVLATLILVQHNNTELTEVKSELSPLNVVLHAEEDTVQIQGKPITEQAWFIPTVAGCFGFSALAIILGLAIGLPIVKRKEKRLLEEKERQEQLAEQLQRISAQQEEQQALEQQAAAEAHAEAEVEPAPQPVPVPPQPQVQINFGPRTGFPPQPGMAPRPGMPPHPGMAPRSGFPPQPGMAPRPGMPPHPGMAPRPGFPPQPGMAPRPGMPPHPGMAPRPGFPPQPGMAPRPGMQPPRPGMPPQPGFPPKR", "aa_hexdigest": "5b2bf3ad1256a2814f7fa2658be17397", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002EA0F8A", "uniref100_id": "UniRef100_A0AB38W863", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002EA0F8A", "UniRef:UniRef100_A0AB38W863" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W863", "uniref90_id": "UniRef90_P75330" }, "psc": { "uniref90_id": "UniRef90_P75330", "gene": "p30", "product": "P30 adhesin", "uniref50_id": "UniRef50_P75330", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035", "GO:0007155" ] }, "pscc": { "uniref50_id": "UniRef50_P75330", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75330" ], "product": "P30 adhesin" }, "genes": [ "p30" ], "id": "GIHJHMOALN_988", "locus": "GCA009939745_00973" }, { "type": "cds", "sequence": "contig_1", "start": 551701, "stop": 552282, "strand": "-", "frame": 1, "gene": null, "product": "MG319/MPN454 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014325547.1", "SO:0001217", "UniParc:UPI0001DD63F5", "UniRef:UniRef100_A0AAX0SQD6", "UniRef:UniRef50_P47561", "UniRef:UniRef90_P75329" ], "nt": "ATGGGTTTCTTCAAATTGCTCTTTAAGCTAGCCTTCTTAGCGGTACTGACGGTAGCAATTGCTTACCTCTTTTTAGCAATCTTTTACTTTGGTACTGTTAGCCCATCCTTTAAACTAGCACAACCAATGGATGTGTTTAACCGTTTTTTCTCTAAGGAAGCGCTTGACACGGTTACTACTAGTGGTGTAGGCGCTACGGTAACTGCACCTAAAGCAGCTGCAGCATCACAGTCCCACGCCTTGATACTAGCAGCAGAACAAGTAGCTAATAATGCTAACAAGCTAGATCCCCGCTTTCCGATTGATGGTAGTTTGTTTAGCCAAATCCCAGGGTATGTCGACTTTTTAAAGAAACCCAATATCCCCGCCTTTTTAGAAAAGGTTGGTCCCTACCTTACTAAGTACATTATTCCGTTGGGCATGGCCTTAGTTTCAGGGCTTATAGGTGCCTTAATTATTAACTTTATCCTGTCCAAGATTACCCGTGCCATCATCAAAAAGCGCCGTAAAGCAAAACGGGCACAACAAAGTCGTGATGATGATTACTACTATGATGAAGCACCCCGTCGCCGCCGTCGTTAG", "aa": "MGFFKLLFKLAFLAVLTVAIAYLFLAIFYFGTVSPSFKLAQPMDVFNRFFSKEALDTVTTSGVGATVTAPKAAAASQSHALILAAEQVANNANKLDPRFPIDGSLFSQIPGYVDFLKKPNIPAFLEKVGPYLTKYIIPLGMALVSGLIGALIINFILSKITRAIIKKRRKAKRAQQSRDDDYYYDEAPRRRRR", "aa_hexdigest": "aed0d4a4d258b1ca7b40e9a5501c3336", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63F5", "ncbi_nrp_id": "WP_014325547.1", "uniref100_id": "UniRef100_A0AAX0SQD6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63F5", "RefSeq:WP_014325547.1", "UniRef:UniRef100_A0AAX0SQD6" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQD6", "uniref90_id": "UniRef90_P75329" }, "psc": { "uniref90_id": "UniRef90_P75329", "product": "MG319/MPN454 family protein", "uniref50_id": "UniRef50_P47561", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47561", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47561" ], "product": "Uncharacterized protein MG319" }, "genes": [], "id": "GIHJHMOALN_989", "locus": "GCA009939745_00974" }, { "type": "cds", "sequence": "contig_1", "start": 552371, "stop": 552673, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAACTTTACGGTGTTCCGCGGTGGTCGCACTAATATCTTGGATTTACTGTTCCCCGGACTTTCGTTAATAGTTGGTGGGATCTTTTCCTTGTTATCGGTTTACTTTGTTTGTCTACAAATTCGCAACGAACAGGACTTGGTGAAACTGTATGAAAGTCAAGACCCAGCTTTAGCCAAAGCACAAATGTGGCGTAGTGCGCTCTTTTTTGGCGCTTGGTTGGTAAGTAGCTTCATGAACTTGGTCTACTTTATCCTGCGCATTATTTTGTTAACGCGTAATTTAAGCAGAGCTTTTTAA", "aa": "MLNFTVFRGGRTNILDLLFPGLSLIVGGIFSLLSVYFVCLQIRNEQDLVKLYESQDPALAKAQMWRSALFFGAWLVSSFMNLVYFILRIILLTRNLSRAF", "aa_hexdigest": "9c4b94a219939d2077e8cef7ce3052bb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11483.538399999992, "isoelectric_point": 9.851107597351074 }, "id": "GIHJHMOALN_990", "locus": "GCA009939745_00975" }, { "type": "cds", "sequence": "contig_1", "start": 552713, "stop": 553234, "strand": "-", "frame": 3, "gene": null, "product": "putative MG320-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47562", "UniRef:UniRef90_P75328" ], "nt": "ATGATCAATCAAGCTAATAATCAGGTCAATTTTTCCGAGCAACAGTTTGTCCATCACAAGCGGTTCTCGGTCATTCGCTTGACCTTTTCTGTTGCCGCGATTGGCATTCTGTTTATCTTCTTAATTGGTTTTGGGGTACAACAGCTGTTAACCAACACTACTAGCCTTGGCACACTGGCTAGTGATATAAGAACGTTGGGTACGATTGCTTTTGTTGCTAGTTTGGTTTCCTTAATCCTTTACTTTGTCACGGCTTTTAAACTAAGGAACCGTAACACGACCTTAGCGTGGTTCTGGGGCTTAATTATTGCCGATGTGATTAGTTATGGGATTACATTAGGAGTATTGTTAACATTAGCCACCACACAATTACGTGAATTGATTGACTTTAAGTTTAGTGACATTGTCTTTGCCTTTTTAGGGGCAGCATTAGTGTATGGGACGGTATGGGGCTTGAGTGCATTACCGAGCCAGCAACGCCGTTACCAACAAACCCAAACGCTGTTTCGTATCTTTATTTGA", "aa": "MINQANNQVNFSEQQFVHHKRFSVIRLTFSVAAIGILFIFLIGFGVQQLLTNTTSLGTLASDIRTLGTIAFVASLVSLILYFVTAFKLRNRNTTLAWFWGLIIADVISYGITLGVLLTLATTQLRELIDFKFSDIVFAFLGAALVYGTVWGLSALPSQQRRYQQTQTLFRIFI", "aa_hexdigest": "a3768fd76e5e46b3b01c7b669d5381d5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 552410, "stop": 553234, "observations": { "mutation": [ 552715 ], "stop": [ 552715 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75328", "query_cov": 1.0, "subject_cov": 0.6027874564459931, "identity": 1.0, "score": 324.0, "evalue": 2.55e-110, "gene_start": 1, "gene_end": 173, "reference_start": 1, "reference_end": 173, "reference_sequence": "MINQANNQVNFSEQQFVHHKRFSVIRLTFSVAAIGILFIFLIGFGVQQLLTNTTSLGTLASDIRTLGTIAFVASLVSLILYFVTAFKLRNRNTTLAWFWGLIIADVISYGITLGVLLTLATTQLRELIDFKFSDIVFAFLGAALVYGTVWGLSALPSQQRRYQQTQTLFRIFIWAFFISIIASLLTFVLNFTVFRGGRTNILDLLFPGLSLIVGGIFSLLSVYFVCLQIRNEQDLVKLYESQDPALAKAQMWRSALFFGAWLVSSFMNLVYFILRIILLTRNLSRAF" }, "paralog": false, "identity": 0.9963503649635036, "score": 526.0, "evalue": 3.06e-192, "description": "Internal stop codon at 552715. Nonsense mutation around 552715.", "psc": { "uniref90_id": "UniRef90_P75328", "product": "Uncharacterized protein MG320 homolog", "uniref50_id": "UniRef50_P47562", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75328", "UniRef:UniRef50_P47562", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47562" ], "product": "Uncharacterized protein MG320" } }, "id": "GIHJHMOALN_991", "locus": "GCA009939745_00976" }, { "type": "tRNA", "sequence": "contig_1", "start": 553304, "stop": 553394, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(gga)", "amino_acid": "Ser", "anti_codon": "gga", "score": 69.6, "nt": "GGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAACACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 553304, 553306 ], "id": "GIHJHMOALN_13", "locus": "GCA009939745_00977" }, { "type": "tRNA", "sequence": "contig_1", "start": 553396, "stop": 553484, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 75.0, "nt": "GGAGACTTACCCAAGTGGTTTAAGGGGGCGCTCTCGAAAAGCGTTAGGTGTTTTTGCATGCGTGGGTTCGAATCCCACAGTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 553430, 553432 ], "id": "GIHJHMOALN_14", "locus": "GCA009939745_00978" }, { "type": "cds", "sequence": "contig_1", "start": 553752, "stop": 554345, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACGCAGCCACCCAATTGAACGATCAAGGTGGCGGTGATGTTATTACCTACTCCTCGGGCGCTCACATTGTACGTACGTTGTTGTTAGCTGCGAGTCAAAAAGATGTACATAGTAAATTCACACAAAAAATAGATCAACAAAATACAGCTTCAACCACATCTGATGTGACTGTTAAGAAAGCAGATTCATCTCAAGATAGCAGCAAGAGTAATACTGAAGAAGAAAAGTGGGATGACGTAACTTCCGCTGACCTCTTCAAAGATGACCCTTACGTACTAAAGAACTTTGGTGATGCTAAAGCACAAGCTGCACAAAGAAGCACCGGTTCTACAACTAGTGGTAACGGCACTCAAGCATCTTTAGAGTTCACCAAAAAGGCTTTAAGCTTGCTCAAATTCTTAGTGGATAATGGCATTCTAGACAAAGAAAAAGTAAAGGAAGCTATCAAAGATCCAAATAAGTACCTTGGAAAGCGTGACAAGATAGAGAACGGTACAAATAAACCAAGTAAAAACGAAGATTTCATTGGTTACGAGTTGAAAGATATCTATAAGAACGCCGCCCAGCTCAACCGCTTTAACAGTATTTGA", "aa": "MDAATQLNDQGGGDVITYSSGAHIVRTLLLAASQKDVHSKFTQKIDQQNTASTTSDVTVKKADSSQDSSKSNTEEEKWDDVTSADLFKDDPYVLKNFGDAKAQAAQRSTGSTTSGNGTQASLEFTKKALSLLKFLVDNGILDKEKVKEAIKDPNKYLGKRDKIENGTNKPSKNEDFIGYELKDIYKNAAQLNRFNSI", "aa_hexdigest": "adf1f0a43c5c833a608a8b021a19c4b0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21577.56740000001, "isoelectric_point": 6.1571065902709945 }, "id": "GIHJHMOALN_992", "locus": "GCA009939745_00979" }, { "type": "cds", "sequence": "contig_1", "start": 554376, "stop": 555011, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAACTCCGGTGACCTGAACTATTCGATCGTACCTTTTGGCATCTTTAAGGAAAAGGGTAAAAATGGTGCAAGTGTTCAACAAAAAGCTGTCTCAACTACCGAAGGTTCAGATCTGATGAACGATTACTACTATAAGATCGAGAAGGAACAGCGCCTGGGCTTGATTCCACAGCGTGAAGGTAACTACGAAAAGAATAAAAACGTTTTGGAGAGTGCGACTGTTAAGATTAATTATTATAGCAGTAAAGCAACAAGTGGTCAGGCTGGAGCAGCTGCCTCTGCAGCGTTTGCTAAGAACAACAACACATCAGACAACACACAACAAAACCAAACCTCTTCAGTAGAAGCTAAGAGTGTAAACGTAACAAAGCACAGCTTTGTTCAAGCTTTAAAGAAGGTTGGTTTCTCTGGTAGCAATCCCCTCCACTTCAACATGAAGTTAGGGAACTCTTCGCTGTCCGCTAACGGGGTGGATTACTACAACGCCGTGAAGCAAGCCCTCACTGAATTGGGTACTGCTGACAACGGAGAAAAACTAATAGTACCAGAAATTATCTTAGGTGATGCCCAAGGACCAACTCGAAATGAGTGGTACATTGGGTTGTCCTCGGTGTTAGGTTTTAGTTCCTGA", "aa": "MPNSGDLNYSIVPFGIFKEKGKNGASVQQKAVSTTEGSDLMNDYYYKIEKEQRLGLIPQREGNYEKNKNVLESATVKINYYSSKATSGQAGAAASAAFAKNNNTSDNTQQNQTSSVEAKSVNVTKHSFVQALKKVGFSGSNPLHFNMKLGNSSLSANGVDYYNAVKQALTELGTADNGEKLIVPEIILGDAQGPTRNEWYIGLSSVLGFSS", "aa_hexdigest": "9040aa5d797a24641725794bf7631afc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22721.937099999992, "isoelectric_point": 8.58217639923096 }, "id": "GIHJHMOALN_993", "locus": "GCA009939745_00980" }, { "type": "cds", "sequence": "contig_1", "start": 555033, "stop": 555488, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGCCTTACTATGTCGAGAGTTTTAACCAGAGCCAGATTGTCTTTAAGCGTAACAGTGAGTATGATACTCATATAACTCCCAACTTACCAAAAACGCGGGAAGAAAACGAAAAACCCATCCCAACGATGATTAACTACTTCCAACCAGGCGCTACCCCTGAAGTGTTTTACTCCAACTATATCGCTGGAGGATTATCTTCTGCGGAGGTGCCATACTCCCAACAAGAGGATGCTAGGTCACGATTTGCGGGTACGGGGGACTTACGCTGGGTTAAAGTGCAAAAGACCGCGCAAAGTGCCCAGATTACCTATTCATCACGACCTTACGTAGTGGAAGGTGAAACCGTTAAAACTAACTCCAACATTACCGAAACCGAGGCTAAATTCTTGTACAACAGTGAGTCAGAAGAAGCGTTAACGATTCGCGCTGGCATTAACGGTTTAATTAACTGA", "aa": "MGPYYVESFNQSQIVFKRNSEYDTHITPNLPKTREENEKPIPTMINYFQPGATPEVFYSNYIAGGLSSAEVPYSQQEDARSRFAGTGDLRWVKVQKTAQSAQITYSSRPYVVEGETVKTNSNITETEAKFLYNSESEEALTIRAGINGLIN", "aa_hexdigest": "751b86784319df3985cddd785068e8b1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17005.5827, "isoelectric_point": 5.081542396545408 }, "id": "GIHJHMOALN_994", "locus": "GCA009939745_00981" }, { "type": "cds", "sequence": "contig_1", "start": 555504, "stop": 555881, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTGGATGTGAAAAAGACCGTGGGGATGACCAAGAAGAATGGTACCCAAGTTCAAATGAAGGTTGCTTCATCTGATGAAAAAGATGGTGAAGAAAAAACTGTTAAGATAACGGATGATGCTTACAATCCTGAGGATTATCAAAGCACTGATGACAGCAAATTCAACGTCTACCTCACCTCGCCCTTCCCCTTCTTGTTGAGTATGATGTCTAAGGAATTCTTCTTTCCTATTCCTCATACCCACCCGAAGGTAAAAGCAATTAAGGTAGGTAAGGACTCACCATTGGTCTACAACGAAAAGAATGGGTCGAAAATCTTAGACCAAACCAAAACTAACTTTGATGGTATCTATGGTGGTGGGGTTAACGCATGA", "aa": "MGLDVKKTVGMTKKNGTQVQMKVASSDEKDGEEKTVKITDDAYNPEDYQSTDDSKFNVYLTSPFPFLLSMMSKEFFFPIPHTHPKVKAIKVGKDSPLVYNEKNGSKILDQTKTNFDGIYGGGVNA", "aa_hexdigest": "42b0ac68c334af1a8071d8df97aa7caf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13890.575299999986, "isoelectric_point": 6.726576423645019 }, "id": "GIHJHMOALN_995", "locus": "GCA009939745_00982" }, { "type": "cds", "sequence": "contig_1", "start": 556170, "stop": 556577, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTCAGCGAAAATACTGGGGTTTGTTATCAACCTTAGGGGTAAGTTCAGCAGTAGCACTCAGTGCCTGCGCTGCCCAAGCCCGCGATGTGTATGTCACCTCATCCGCATCCGACTTGTTAAAGAACAATTCGGTACCGATGAGTATGTTCAACGTGTCCCCGACCTCTTCCTTTTTTGGCAGCAAGTATGCAGGCCTAACCACTTACATTGCCACTGGTTCCAACAAAGATGATGGTGTCAATGTAACCACTCAAACCCAAGAGAAATTGGTGTTGGAATTGGCTACCTCAGTGAAGGGGTATAAGAAGAAGGATAAAGCTACTTCAAGTCAAAAAACTTCAACTAATTCTTCTTGCACTACCACTTCTAGTGGTACTAGCACTAGTGGTGAAGATGATTGA", "aa": "MKFQRKYWGLLSTLGVSSAVALSACAAQARDVYVTSSASDLLKNNSVPMSMFNVSPTSSFFGSKYAGLTTYIATGSNKDDGVNVTTQTQEKLVLELATSVKGYKKKDKATSSQKTSTNSSCTTTSSGTSTSGEDD", "aa_hexdigest": "2377da1e52e23b097a7147ead82f8f3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14212.632599999977, "isoelectric_point": 9.098052406311037 }, "id": "GIHJHMOALN_996", "locus": "GCA009939745_00983" }, { "type": "tRNA", "sequence": "contig_1", "start": 556801, "stop": 556885, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 64.4, "nt": "GCACTCGTGGCGGAATGGTAGACGCGCTAGACTTAGGATCTAGTTTCATTGCGGAGTGGGGGTTCAAGTCCCTTCGAGTGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 556865, 556867 ], "id": "GIHJHMOALN_25", "locus": "GCA009939745_00984" }, { "type": "tRNA", "sequence": "contig_1", "start": 556890, "stop": 556965, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 85.2, "nt": "GCATCTTTAGCTCAGTTGGTAGAGCAAATGACTCTTAATCATTGGGTCGGGGGTTCGAGCCCCTCAAGATGCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 556959, 556961 ], "id": "GIHJHMOALN_24", "locus": "GCA009939745_00985" }, { "type": "tRNA", "sequence": "contig_1", "start": 556978, "stop": 557053, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 89.8, "nt": "GCCGACTTAGCTCAGTTGGTAGAGCAATTGACTTGTAATCAGTAGGTCGTAGGTTCGAATCCTATAGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 557018, 557020 ], "id": "GIHJHMOALN_23", "locus": "GCA009939745_00986" }, { "type": "tRNA", "sequence": "contig_1", "start": 557055, "stop": 557130, "strand": "-", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 81.7, "nt": "GGATTGTTAGCTCAGCTGGGAGAGCATATGCCTTACAAGCATAGGGTCGGGGGTTCGATCCCCTCACAATCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 557095, 557097 ], "id": "GIHJHMOALN_22", "locus": "GCA009939745_00987" }, { "type": "tRNA", "sequence": "contig_1", "start": 557138, "stop": 557212, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 81.5, "nt": "GCCGAATTAGCTCAAGGGTAGAGCGATGCACTCGTAATGCATAGGCTACAGGTTCAATTCCTGTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 557178, 557180 ], "id": "GIHJHMOALN_21", "locus": "GCA009939745_00988" }, { "type": "tRNA", "sequence": "contig_1", "start": 557214, "stop": 557290, "strand": "-", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 58.9, "nt": "GGCGCGTTGGTGAAGAGACTTAACACACACGCCTTTCACGCGTGCATTCACGGGTTTGAATCCCGTACGCGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 557254, 557256 ], "id": "GIHJHMOALN_20", "locus": "GCA009939745_00989" }, { "type": "tRNA", "sequence": "contig_1", "start": 557299, "stop": 557373, "strand": "-", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 84.0, "nt": "GGCCACATAGCTCAGCGGTAGAGCAACCGGCTGTTAACCGGTTGGTCACAGGTTCGATCCCTGTTGTGGCCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 557339, 557341 ], "id": "GIHJHMOALN_19", "locus": "GCA009939745_00990" }, { "type": "cds", "sequence": "contig_1", "start": 557401, "stop": 557880, "strand": "-", "frame": 1, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027D97117", "UniRef:UniRef90_UPI0027D97117" ], "nt": "ATGGTGATTGCGATGTTTATCGGGGGCAGTCCGTCTTCTACCGCCGGAGGGATTCGCACTACTACTTTAGCGGTCATCTTTTTGACGTTAAAAGCGAAGTTCCGCGGTCAAAAAGAGGTCAAGGTCTTTAAGCGTAGTATTGACGGGCAAACGGTGATTAATGCCTTCTTGGTAGCCGTCTTTGGCTTAGTGTCCGTGGTTCTTATTGCCATTCTGTTGCCGTTAAGTATGCAACAACCGCTTAGCTTTGTCGACAGCCTGTTTGAAACCACTTCTGCTTTTGGAACTGTCGGACTGTCTAGTGGGGCCACGAAGATCATGGCACTAGAGCCAACACGGAACCTGTTTAACTACTTAACGTTAGGTTTACTAATGATTATGGGGCAGGTCGGGGTGAGCAGTTCGGTACTCACTTTTGTTAAAAAACACCCTCAAGGAAACAGCTTTTCTTATCCGCGGGAAGATGTTAAAGTGGGCTAG", "aa": "MVIAMFIGGSPSSTAGGIRTTTLAVIFLTLKAKFRGQKEVKVFKRSIDGQTVINAFLVAVFGLVSVVLIAILLPLSMQQPLSFVDSLFETTSAFGTVGLSSGATKIMALEPTRNLFNYLTLGLLMIMGQVGVSSSVLTFVKKHPQGNSFSYPREDVKVG", "aa_hexdigest": "8d738b66a126f157973e098d96f98f66", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 557401, "stop": 558039, "observations": { "mutation": [ 557913 ], "stop": [ 557913 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0027D97117", "query_cov": 1.0, "subject_cov": 0.7464788732394366, "identity": 1.0, "score": 295.0, "evalue": 3.01e-100, "gene_start": 1, "gene_end": 159, "reference_start": 55, "reference_end": 213, "reference_sequence": "VLKNKSNQELQSLIFGPIPAASRVMQLWFGVISSRSAGFSVFPWSAESDIIKGIMVIAMFIGGSPSSTAGGIRTTTLAVIFLTLKAKFRGQKEVKVFKRSIDGQTVINAFLVAVFGLVSVVLIAILLPLSMQQPLSFVDSLFETTSAFGTVGLSSGATKIMALEPTRNLFNYLTLGLLMIMGQVGVSSSVLTFVKKHPQGNSFSYPREDVKVG" }, "paralog": false, "identity": 0.9952830188679245, "score": 400.0, "evalue": 7.11e-144, "description": "Internal stop codon at 557913. Nonsense mutation around 557913.", "psc": { "uniref90_id": "UniRef90_UPI0027D97117", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI0027D97117", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0027D97117", "UniRef:UniRef50_UPI0027D97117" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0027D97117", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027D97117" ], "product": "potassium transporter TrkG" } }, "id": "GIHJHMOALN_997", "locus": "GCA009939745_00991" }, { "type": "cds", "sequence": "contig_1", "start": 557911, "stop": 558660, "strand": "-", "frame": 1, "gene": "trkG", "product": "putative cation transporter MG322-like protein", "db_xrefs": [ "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001", "SO:0001217", "UniRef:UniRef50_P75323", "UniRef:UniRef90_P75323" ], "nt": "ATGTTGCAATCAGAACGGGGTGGTTCCAAGTTAGGGACCACTAGTGAAATGATTGTGGTGTCGGTGTTATTCCTCTTTATGGTGGAGTTACTGTATGGCTTTCTGTACACCATTTTGTTTTATTTTATCCCCGCGTTTGAGTCTGCTAGTGTTTTTCAAAGTAGTGGTAAGGTGTCCAACCAATTGAAGGCCCTCATAGTCGATTCCACCAAAAGATTACCCGTAGTTCATAACCTTAACTTAGCCTTTCAGTATGGTTTTTTCCACTCCTTGTCAGCGGTTAATAATGCTGGCATTGACCTGTTGGGCGCTAATTCGTTTGCCCCGTATCGCACTAACTGGGGCATCGTGATCCAGTGGTTAGCGATTAGCCAAATTATCTTTGGTGGCATTGGTTATCCGGTGTTATTTGATGCTTATGAAGCGATTAAGAAACGGCGATTGTATGGTAAGTATTACAAACACCAGTTTAGTTTGTTTACGAAGTTAGCGGTACTCACTAACCTGATTGTCACGGCTTGGTGTTTTTTAATGCTGTTAATGGTGGAGTTTATTGTTATAACTAGTTTGACCAATACGATTGCCCATCTCAATGTCGAGAAGGCCTACTTAGTGGAGGGACTGAAAAATAAATCCAATCAAGAACTCCAAAGCTTAATCTTTGGTCCCATACCAGCGGCTAGTCGGGTGATGCAACTGTGGTTTGGGGTGATTTCGTCCCGGTCCGCTGGCTTTAGTGTGTTTCCCTGA", "aa": "MLQSERGGSKLGTTSEMIVVSVLFLFMVELLYGFLYTILFYFIPAFESASVFQSSGKVSNQLKALIVDSTKRLPVVHNLNLAFQYGFFHSLSAVNNAGIDLLGANSFAPYRTNWGIVIQWLAISQIIFGGIGYPVLFDAYEAIKKRRLYGKYYKHQFSLFTKLAVLTNLIVTAWCFLMLLMVEFIVITSLTNTIAHLNVEKAYLVEGLKNKSNQELQSLIFGPIPAASRVMQLWFGVISSRSAGFSVFP", "aa_hexdigest": "2e8f4cfaf0c0bd006df29af5d3a18073", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "both", "pseudogene": { "start": 557608, "stop": 558960, "observations": { "mutation": [ 557913, 558714 ], "stop": [ 557913, 558714 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75323", "query_cov": 1.0, "subject_cov": 0.43915343915343913, "identity": 1.0, "score": 477.0, "evalue": 4.5e-165, "gene_start": 1, "gene_end": 249, "reference_start": 147, "reference_end": 395, "reference_sequence": "MTAAHKQKAKLLAWLKLILWGDSISQRIFHFYIYCILLGAVLLFLPFALKTDYQKVISYEVDLQGHTISKQTASYGFLDALFLAVSAFSDTGLSTTVVSETYSVFGQTVLAILLQLGGIGFVVIAFLVWRLFKLHKKGKYSFYEKLMLQSERGGSKLGTTSEMIVVSVLFLFMVELLYGFLYTILFYFIPAFESASVFQSSGKVSNQLKALIVDSTKRLPVVHNLNLAFQYGFFHSLSAVNNAGIDLLGANSFAPYRTNWGIVIQWLAISQIIFGGIGYPVLFDAYEAIKKRRLYGKYYKHQFSLFTKLAVLTNLIVTAWCFLMLLMVEFIVITSLTNTIAHLNVEKAYLVEGLKNKSNQELQSLIFGPIPAASRVMQLWFGVISSRSAGFSVFPWSAESDIIKGIMVIAMFIGGSPSSTAGGIRTTTLAVIFLTLKAKFRGQKEVKVFKRSIDGQTVINAFLVXXXVFGLVSVVLIAILLPLSMQQPLSFVDSLFETTSAFGTVGLSSGATKIMALEPTRNLFNYLTLGLLMIMGQVGVSSSVLTFVKKHPQGNSFSYPREDVKVG" }, "paralog": false, "identity": 0.9889380530973452, "score": 851.0, "evalue": 8.44e-315, "description": "Internal stop codon at 557913, 558714. Nonsense mutation around 557913, 558714.", "psc": { "uniref90_id": "UniRef90_P75323", "gene": "TrkG", "product": "Uncharacterized cation transporter MG322 homolog", "uniref50_id": "UniRef50_P75323", "cog_id": "COG0168", "cog_category": "P", "go_ids": [ "GO:0005886", "GO:0008324", "GO:0030001" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75323", "UniRef:UniRef50_P75323", "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001" ] }, "pscc": { "uniref50_id": "UniRef50_P75323", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75323" ], "product": "Uncharacterized cation transporter MG322 homolog" } }, "id": "GIHJHMOALN_998", "locus": "GCA009939745_00992" }, { "type": "cds", "sequence": "contig_1", "start": 558712, "stop": 559098, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGCTGCTCATAAACAGAAAGCTAAACTACTCGCTTGGTTAAAACTAATCCTGTGGGGTGACAGTATTTCCCAGCGCATTTTCCACTTTTACATCTATTGCATCCTGTTGGGGGCTGTACTGCTTTTCCTCCCGTTTGCGCTCAAAACCGACTACCAAAAGGTTATTAGCTATGAGGTGGATTTACAGGGTCATACCATCAGTAAACAAACGGCTTCCTATGGCTTTTTGGATGCTTTGTTTTTAGCGGTCAGTGCCTTTAGTGATACGGGGCTCTCTACTACGGTAGTGAGTGAAACCTACAGTGTGTTTGGGCAAACGGTGTTGGCCATTTTACTGCAGTTAGGGGGCATTGGTTTTGTGGTGATTGCTTTCCTTGTTTGA", "aa": "MTAAHKQKAKLLAWLKLILWGDSISQRIFHFYIYCILLGAVLLFLPFALKTDYQKVISYEVDLQGHTISKQTASYGFLDALFLAVSAFSDTGLSTTVVSETYSVFGQTVLAILLQLGGIGFVVIAFLV", "aa_hexdigest": "ebe8dac325ed4fa585280446d063174f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14113.476699999981, "isoelectric_point": 7.861161994934083 }, "id": "GIHJHMOALN_999", "locus": "GCA009939745_00993" }, { "type": "cds", "sequence": "contig_1", "start": 559133, "stop": 559828, "strand": "+", "frame": 2, "gene": "trkA", "product": "putative MG323-like protein", "db_xrefs": [ "COG:COG0569", "COG:PT", "GO:0006813", "GO:0008324", "RefSeq:WP_010874817.1", "SO:0001217", "UniParc:UPI000013961A", "UniRef:UniRef100_P75322", "UniRef:UniRef50_P75322", "UniRef:UniRef90_P75322" ], "nt": "ATGAAACGTGCCGATTTTTGCATTATTGGCCTTGGTAGGTTTGGGATGCAAGTAGCACATTCTTTAAAAGAGAACAACTTTACCCTGGTTTTGATCGATAATGATCAACACAAAACCAATACTGCTGCCCAGGAATTTGACCATGTAGTGTGTTGTGATGGGAGTAACTTAACGGCGTTAGCTGAGCTCCAACTAGAGGAGTTTTCGGCAGTCATAGTTGGGGTAACTAACATAGAAGCGAGCATTATGATTTGTGCTAACCTTCGGGAATTGGGTCAAAAAAACATTATTGCCCGTGCTAAAAACGAAGTGCATAACCGGGTGTTACGTACTATGGGTATTAGGGAAGCTTTGATCCCGGAAAAGATTGTCGGCAAGAACCTTGTCATTCGTTTAATTCACGGCATGGAAACCGAAATTATTAACCTTGGCAATGACATCCTCTTTGTTCGCGCTCCGGTGAACAACAAGAAGCTCTTTAATCGTACGTTGGGGGAAATTAACATCCGTGAACACACGAATGCTAACCTCATCTCAATTATGCGGAATGGCAAAGATGTCGTCTTTCCTTTGGGTCCGAATACCCAGATTCAACCAGGGGATGTAATTACAGCCGTGTGTCAATTAAGTGGCGTGAACCAGTATTTGCGCTACATTAATCCTAACGACAAGAATAAGTACAAAGCGAGTGAATAA", "aa": "MKRADFCIIGLGRFGMQVAHSLKENNFTLVLIDNDQHKTNTAAQEFDHVVCCDGSNLTALAELQLEEFSAVIVGVTNIEASIMICANLRELGQKNIIARAKNEVHNRVLRTMGIREALIPEKIVGKNLVIRLIHGMETEIINLGNDILFVRAPVNNKKLFNRTLGEINIREHTNANLISIMRNGKDVVFPLGPNTQIQPGDVITAVCQLSGVNQYLRYINPNDKNKYKASE", "aa_hexdigest": "ed910f8d08c6738e9a02d4fb3d1effa7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013961A", "ncbi_nrp_id": "WP_010874817.1", "uniref100_id": "UniRef100_P75322", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013961A", "RefSeq:WP_010874817.1", "UniRef:UniRef100_P75322" ] }, "ips": { "uniref100_id": "UniRef100_P75322", "uniref90_id": "UniRef90_P75322" }, "psc": { "uniref90_id": "UniRef90_P75322", "gene": "TrkA", "product": "Uncharacterized protein MG323 homolog", "uniref50_id": "UniRef50_P75322", "cog_id": "COG0569", "cog_category": "PT", "go_ids": [ "GO:0008324", "GO:0006813" ] }, "pscc": { "uniref50_id": "UniRef50_P75322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75322" ], "product": "Uncharacterized protein MG323 homolog" }, "genes": [ "trkA" ], "id": "GIHJHMOALN_1000", "locus": "GCA009939745_00994" }, { "type": "cds", "sequence": "contig_1", "start": 559890, "stop": 560324, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MPN_462", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321", "UniRef:UniRef50_UPI001375E5B8", "UniRef:UniRef90_P75321" ], "nt": "GTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCAGACAGCACTAACCAAACAGGCGCAATGTTTGGCTTGAAGGTGAAGGATGCAACCGTGGATAGTTCGAAGCAATCAACGGAAAGCTTAAAGGGCGAAGAATCGAGTTCCAGTTCCACCACATCTTCCACCTCCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGA", "aa": "MVYYEQLQRGLALPNQGSSSGSDSTNQTGAMFGLKVKDATVDSSKQSTESLKGEESSSSSTTSSTSTTQRGGSSGDTKVKALQVAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTAPSSSESGQSGTPTP", "aa_hexdigest": "41565d62a1343546e1f95d063ffe9e18", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001375E5B8", "query_cov": 0.9097222222222222, "subject_cov": 0.8618421052631579, "identity": 0.6559999999999999, "score": 142.0, "evalue": 1.13e-40, "valid": false, "uniref50_id": "UniRef50_UPI001375E5B8" }, "pscc": { "uniref50_id": "UniRef50_UPI001375E5B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001375E5B8" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 559890, "stop": 560627, "observations": { "mutation": [ 560322, 560370, 560484, 560499, 560523 ], "stop": [ 560322, 560370, 560484, 560499, 560523 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75321", "query_cov": 1.0, "subject_cov": 0.48, "identity": 0.993, "score": 226.0, "evalue": 9.11e-72, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MVYYEQLQRGLALPNQGSSSGSDSTNQTGAMFGLKVKDATVDSSNQSTESLKGEESSSSSTTSSTSTTQRGGSSGDTKVKALQVAVKKKSDSEDNGQIELETNNLANAPIKRGSNNNQQVQLKADDFGTAPSSSESGQSGTPTPWTPWLATEQIHKDLPKWSASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDQGQAVADNTQTGFDTDDTDNKKQGFRKQGEQSPAPIALPFEAYFANIGNLTWFGQALLVFGICLS" }, "paralog": false, "identity": 0.9714285714285714, "score": 434.0, "evalue": 2.66e-156, "description": "Internal stop codon at 560322, 560370, 560484, 560499, 560523. Nonsense mutation around 560322, 560370, 560484, 560499, 560523.", "psc": { "uniref90_id": "UniRef90_P75321", "product": "Uncharacterized protein MPN_462", "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75321", "UniRef:UniRef50_P75321" ] }, "pscc": { "uniref50_id": "UniRef50_P75321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75321" ], "product": "Uncharacterized protein MPN_462" } }, "id": "GIHJHMOALN_1001", "locus": "GCA009939745_00995" }, { "type": "cds", "sequence": "contig_1", "start": 560304, "stop": 560759, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCGAACCAGGTGAGGTTGCCAATGTTGGCGAAGTACGCTTCAAACGGGAGGGCGATGGGGGCAGGGGATTGTTCGCCTTGTTTGCGAAAGCCCTGCTTTTTATTATCGGTGTCATCTGTATCAAACCCGGTTTGGGTATTATCCGCGACCGCTTGGCCCTGGTCAAACCACTCTGGGTGGCGGCGCGGGTTGAAGAACCCGGTGGTTTGGAGCAGGACATCGCGCGCCTTTCAGTCCCACAACCCGTGGTGGTTTCACTTGGGCGTTCTTCAACTGGGCGGATAGTTCGCGGTCATCACCTTGGGATCCAAGTGATCAACGCGATCAATGGCGGTACGATTGCGCGCATAAGGCGCATCGTACAGAATCAGGATCGAGGCGGATCATTTGGGGAGGTCCTTGTGAATTTGCTCGGTCGCAAGCCACGGCGTTCAGGGGGTGGGGGTGCCTGA", "aa": "MPEPGEVANVGEVRFKREGDGGRGLFALFAKALLFIIGVICIKPGLGIIRDRLALVKPLWVAARVEEPGGLEQDIARLSVPQPVVVSLGRSSTGRIVRGHHLGIQVINAINGGTIARIRRIVQNQDRGGSFGEVLVNLLGRKPRRSGGGGA", "aa_hexdigest": "a30dbf08a756d3c04d6bcdca05587bd0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16026.549699999983, "isoelectric_point": 11.366952705383298 }, "id": "GIHJHMOALN_1002", "locus": "GCA009939745_00996" }, { "type": "cds", "sequence": "contig_1", "start": 560935, "stop": 561510, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGTCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAACTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGTCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCAGAAGTTAAATGAAGCTTGA", "aa": "MGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQVLQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYKTLQDLLVEQPVTPYTPNAGLVRVNGVAQDTVHFGSGQESSWNSQRSQKGLKNNPGPKAVTGFKLDKGRAYQKLNEA", "aa_hexdigest": "ca647ad375acb6498a92f0869d1fadab", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20772.74409999999, "isoelectric_point": 9.396218681335448 }, "id": "GIHJHMOALN_1003", "locus": "GCA009939745_00997" }, { "type": "cds", "sequence": "contig_1", "start": 561514, "stop": 561840, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAACCCCTGGATTCGACGAAGGAGGGGAAGGAGAAAAACGAATCGGAGTGGAAGTCTTCCGGGGCGAGTGAGGCAAAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCTGTTACAGACAATCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCACAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MYEPLDSTKEGKEKNESEWKSSGASEAKNDAPLVGSTGSQMAAVTDNQQSGDNNGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTTQALHQMGVIVPSLET", "aa_hexdigest": "c75d6f54ce32396215e55f305233594a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11411.227499999995, "isoelectric_point": 4.809567832946779 }, "id": "GIHJHMOALN_1004", "locus": "GCA009939745_00998" }, { "type": "cds", "sequence": "contig_1", "start": 562144, "stop": 562782, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI002246AD21" ], "nt": "TTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGGGCAGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGTGCTCGCCTGGTCGACCACGGCCGGATTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGATTGAACGGACCGATTAATGGCTTGTTTACCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCCAAAATTGCTTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACATCCGATGATAATTCAAACACCAAAGTCAAGTGA", "aa": "MGFKDDSNSDSKNSNLKAQGLTQPAYLIAGLDVVADHLVFAAFKAGAVGYDMTTDSNASTKDQVLAWSTTAGLDSDGGYKALVENTAGLNGPINGLFTLLDTFAYVTPVSGMKGGSKNTEAVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLDEARLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVK", "aa_hexdigest": "6c57a213ded435cc3e8788e92409378d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002246AD21", "query_cov": 0.9292452830188679, "subject_cov": 0.8565217391304348, "identity": 0.9390000000000001, "score": 350.0, "evalue": 7.6e-121, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "GIHJHMOALN_1005", "locus": "GCA009939745_00999" }, { "type": "cds", "sequence": "contig_1", "start": 563536, "stop": 563898, "strand": "+", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_100", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254", "UniRef:UniRef90_P75592" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAAACTCAAACAACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGCGAGCAAATTAACAAACTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAGAGAACTTCAAGTAGAGCAAAAATTTCAGGGTGAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCGCTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAG", "aa": "MNTEYVTRKEFNEYKNSNNQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIRELQVEQKFQGEQIKAQGKTLKSILQALGGINKRLDKIDPPK", "aa_hexdigest": "4dbddefcbd4c1ec3dd8b4b788f5295c9", "start_type": "GTG", "rbs_motif": "AACAA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 563368, "stop": 563898, "observations": { "mutation": [ 563449 ], "stop": [ 563449 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75592", "query_cov": 1.0583333333333333, "subject_cov": 0.6939890710382514, "identity": 0.8190000000000001, "score": 76.6, "evalue": 4.32e-15, "gene_start": 1, "gene_end": 120, "reference_start": 57, "reference_end": 183, "reference_sequence": "MKEKIPFYNEKEFHDMVKKTKKGTFSGWYIIDKDNKSVEFSGSFNRQFKLNKPVIPVNTEYVTRKEFNEYKVSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQVEQKAQGEQIKAQGKQIKAQGKTLKSILQALGGINKRLDKIDPPK" }, "paralog": false, "identity": 0.8415300546448088, "score": 182.0, "evalue": 5.28e-59, "description": "Internal stop codon at 563449. Nonsense mutation around 563449.", "psc": { "uniref90_id": "UniRef90_P75592", "product": "UPF0134 protein MPN_100", "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75592", "UniRef:UniRef50_P75254" ] }, "pscc": { "uniref50_id": "UniRef50_P75254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75254" ], "product": "UPF0134 protein MPN_524" } }, "id": "GIHJHMOALN_1006", "locus": "GCA009939745_01000" }, { "type": "cds", "sequence": "contig_1", "start": 564364, "stop": 564453, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCGAAAGATTGAACATTTCCAGATGACATACCAAAGTTTTAAGGATTTGTCAGTTGAGGATAAGTTAAGCTATACCTTTAATTGA", "aa": "MERKIEHFQMTYQSFKDLSVEDKLSYTFN", "aa_hexdigest": "2a16b359f26e6c4d1c4f87aadc43554c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3616.038300000001, "isoelectric_point": 5.53852787017822 }, "id": "GIHJHMOALN_1007", "locus": "GCA009939745_01001" }, { "type": "cds", "sequence": "contig_1", "start": 564541, "stop": 564789, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75316", "UniRef:UniRef90_P75316" ], "nt": "ATGCGCAAAAAGAGATTGTTGTCAAGGATTTCCTTTAGTTCTCTATTTTTATTATGTGGAACATTGTTAAGTGCTTGCACTGGTATCCAAGCAGATCTTAGAAATCTTATTAAAGAAACCACTGGTAAAGACATTGATTTGTCTAAAGCAATTAAAACTAAAGAAGGCAAAAAGAACATAATAGCTTCTTTAAAAAAGTCTTACGAAGTTAATCCGAGAGATACAACAAAATTGTTGTTAGATGCTTGA", "aa": "MRKKRLLSRISFSSLFLLCGTLLSACTGIQADLRNLIKETTGKDIDLSKAIKTKEGKKNIIASLKKSYEVNPRDTTKLLLDA", "aa_hexdigest": "13dfe1101522dd7d52ba9752cb93c633", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 1.0, "score": 153.0, "evalue": 5.5e-47, "valid": true, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "GIHJHMOALN_1008", "locus": "GCA009939745_01002" }, { "type": "cds", "sequence": "contig_1", "start": 565186, "stop": 565383, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7", "UniRef:UniRef90_A0AAN4XEA6" ], "nt": "ATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGATACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGTTGACACTGAGACGACAAATAAAGAAAAGCCTTTAGGCACTTGA", "aa": "MKKVKALRNLFLEKASEKLEYPKIASMVAYFDPKEDDTCEFIMKIKTVNEVVDTETTNKEKPLGT", "aa_hexdigest": "9f1295d6f8b633aff52d35b521d941bb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XEA6", "query_cov": 1.0, "subject_cov": 0.8552631578947368, "identity": 0.9540000000000001, "score": 118.0, "evalue": 9.83e-34, "valid": true, "uniref50_id": "UniRef50_A0A0H3DJX7" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DJX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DJX7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7498.6667, "isoelectric_point": 6.245093345642091 }, "id": "GIHJHMOALN_1009", "locus": "GCA009939745_01003" }, { "type": "cds", "sequence": "contig_1", "start": 565422, "stop": 565754, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCACCCACAGTCGTCGTCTGACTGTCACCAAGCGTGCCCTGGACATTCGCTTTGAGACCAACGCCGACATTGCTAAAAGTGGGTGCAACATCATTGGACTGCGTGGTGGAACTTGTGTTGCCCGCCGGCGAGCCGTTGGTGGCGCTGTGACCCGCTCCAGCGACCCCCTTATATCCATTCCCATACCTGTACCCATTCCCAATCCAGGGAATATAATCCAACACCTGGTCAAGGAGTGCCCCCGCGAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAATAAAAATACCAACAGCAAGTTTTAACACTTACCGTTGGTAA", "aa": "MATHSRRLTVTKRALDIRFETNADIAKSGCNIIGLRGGTCVARRRAVGGAVTRSSDPLISIPIPVPIPNPGNIIQHLVKECPREVEPGGETGKRGGEIKIPTASFNTYRW", "aa_hexdigest": "69588fd5fd7208701a1d7b0c2b2ba7aa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11887.59829999999, "isoelectric_point": 10.277694892883297 }, "id": "GIHJHMOALN_1010", "locus": "GCA009939745_01004" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 566216, "stop": 566545, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 265.8, "evalue": 6.9e-29, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "GCTGTCGGTCAGTTGCTGCTCAATGAGTAGAGGAAAGTCCATGCTTGCACTTGCTGGAACGCAAGTAGTGTTTGTGTAAATTGAACAAATAAGATTTAGGACGCTAATAGACGTCACGACTGGACTAGTCTAAGGTCTTTGACTATGATGATGTCCGTAAAGTACCACAGAGACGAGCTTAGTGAAAACTAAGTATGAAACGCGGTAAACTCCACAAGCAAGAAACCTAAATTTTGGTAAGGGAATGTTACTTTAAGAAAAATAATTAAAGTAATGACAGGATTACTGTAGATAAATAACTGACTTTAAAACAGAACATGGCTTATTGGC", "id": "GIHJHMOALN_41", "locus": "GCA009939745_01005" }, { "type": "tmRNA", "sequence": "contig_1", "start": 566570, "stop": 566956, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 566653, "stop": 566733, "aa": "DKNNDEILVDPMLIANQQASINYAFA", "nt": "GACAAAAATAACGACGAAATTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAG" }, "nt": "GGGGATGTAGAGGTTTTGACATAATGTTGAAAGGAAAACAGTGGCAGTGGGGTATGCCCCTTACAGCTCTAGGTATAATAACCGACAAAAATAACGACGAAATTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAGAACATACTAAAGCTACACGAATTGAATCGCCATAGTTTGGTTCGTGTCACAGTTTATGGCTCGGGGTTAACTGGTTCAACTTAATCCTTAAATTATGAACTTATCGTTTACTTGTTTGTCTTATGATCTAAAGTAAGCGAGACATTAAAACATAAGACTAAACTGTAGAAGCTGTTTTACCAATCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA", "id": "GIHJHMOALN_37", "locus": "GCA009939745_01006" }, { "type": "cds", "sequence": "contig_1", "start": 567047, "stop": 567262, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTCTTTTTGGTGTTATCGGACCAACACTTTTTCCCGCGTACGACAACGCAACTAAAAAGTAATCCTAACCTGTCACTGTTCTTTCGGATTTCTCACAAAGACAACGTTTTTAGTTCGCGCTTAAACCAACTAGGAGCGTTTGCCACCGCTTTATGTATTACGCTTGTGGTGTATGAATTGCCTTTTTTAGGACTGTTTGCCTTTAACTGA", "aa": "MLLFLVLSDQHFFPRTTTQLKSNPNLSLFFRISHKDNVFSSRLNQLGAFATALCITLVVYELPFLGLFAFN", "aa_hexdigest": "5ce299020c964f1922fcedb3f2bebbe7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8125.423799999997, "isoelectric_point": 9.18495578765869 }, "id": "GIHJHMOALN_1011", "locus": "GCA009939745_01007" }, { "type": "cds", "sequence": "contig_1", "start": 567494, "stop": 567730, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAGATCAACGATAAACTCACCGAAATTAATACCGTTGAATACGAAGTTGCTTCCAAACACAGTCAGTACCTCTTCTACAGCCGTTTTGGTCTGTTAGATACCGCTGCTTACTTTTTGTTCTTGCTGTCTTTTTTTGTGACAGCAGTGATGTTCTTGGTGGGTATCTTTCACACCGAGCAGTTTACCTTAAACGATCAAAACCAGGGCATTAGTGGTTTTTACTTGTTTTGA", "aa": "MSKINDKLTEINTVEYEVASKHSQYLFYSRFGLLDTAAYFLFLLSFFVTAVMFLVGIFHTEQFTLNDQNQGISGFYLF", "aa_hexdigest": "2d8ba9e1b0fd9fff7c4b0f778e406837", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9082.277099999992, "isoelectric_point": 5.006230735778809 }, "id": "GIHJHMOALN_1012", "locus": "GCA009939745_01008" }, { "type": "cds", "sequence": "contig_1", "start": 567771, "stop": 568835, "strand": "-", "frame": 2, "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "db_xrefs": [ "COG:COG0006", "COG:E", "EC:3.4.11.9", "GO:0004177", "GO:0006508", "GO:0008237", "GO:0046872", "RefSeq:WP_014325558.1", "SO:0001217", "UniParc:UPI0001DD63FB", "UniRef:UniRef100_A0AAX0SPT1", "UniRef:UniRef50_P47566", "UniRef:UniRef90_P75313" ], "nt": "ATGCATAACGAACTGCAACAAAAGCTAGCAGTTTTACACAAACTGCTCCAAGACAATAAAGCTGATGCCATCTTAATTGGCTCAGACCAAAACCGCTTTTGGCTTACTGGTTTTCCCTCCAGTGCTGGTTGGTTAGTGGTTCACAAGCAAAGGGTTAACCTCTTTATTGACGGGCGTTACTTTGAAGCCGCTAAAACGGCAATTGATCCCCTAGTCAAAGTGGAATTATTTACCACTTATAAACAAGTAAAGGCATTATGTGAACAAGTGGGTGTGAAACACCTCTTGATTGAAGGGGATTACCTCACTTTTAACTACCAAAATTTCATTAAGAAGTTGTGTGCGCAGTACACGGTCATTAACGCCCAGGAAATTCGCCGACAAAAGCTCCCGAGTGAAATCTTAGCGATTGAAAAGGTAGTGGAAATTACCCGCAAAGTAGCGGTAAAACTCAAGCGCTTTATCCAACCAGGCATGACGGAGTTGTTCATTGCGCAGTGGATTACTGACCAATTGGTCAAAGCTGGTGGGGCAAAAAACTCGTTTGACCCTATTGTAGCTACTGGTAAGAACGGTGCTAATCCACACCACAAGCCTTCCAAGTTAAAGGTAAAATCAGGTGATTTTGTAACCTGTGACTTTGGCACCATTTACAACGGTTACTGTTCCGATATTACCCGCACCTTCTTAGTTGGTAAAAAACCTAATAATGAAGTGTTGCTCAAGGCCTATAAAAAGGTTGATGAAGCCAATATGGCCGGCATTAACGCTGCTAATACCCAACTCACCGGTGCGGAAGTTGACAAGGTGTGCCGTGACATTATTGAAGCATCGGAGTTCAAAGATTACTTTGTTCACAGCACTGGTCATGGTGTTGGACTTGACATTCACGAAATGCCAAACGTATCAACGTCCTATAACAAGTTGTTGTGTGAAAATGCTGTCATTACGATTGAACCGGGTATTTATATCCCTGGTGTTGGTGGCATCCGCATTGAGGACATGGTGTTAGTTAAAGACCACAAGAGTGTGTGGTTATCAGCCAAGATTCCGCGTGCCTTCTAA", "aa": "MHNELQQKLAVLHKLLQDNKADAILIGSDQNRFWLTGFPSSAGWLVVHKQRVNLFIDGRYFEAAKTAIDPLVKVELFTTYKQVKALCEQVGVKHLLIEGDYLTFNYQNFIKKLCAQYTVINAQEIRRQKLPSEILAIEKVVEITRKVAVKLKRFIQPGMTELFIAQWITDQLVKAGGAKNSFDPIVATGKNGANPHHKPSKLKVKSGDFVTCDFGTIYNGYCSDITRTFLVGKKPNNEVLLKAYKKVDEANMAGINAANTQLTGAEVDKVCRDIIEASEFKDYFVHSTGHGVGLDIHEMPNVSTSYNKLLCENAVITIEPGIYIPGVGGIRIEDMVLVKDHKSVWLSAKIPRAF", "aa_hexdigest": "935fcd33c50928d84f1d840212a7b301", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63FB", "ncbi_nrp_id": "WP_014325558.1", "uniref100_id": "UniRef100_A0AAX0SPT1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63FB", "RefSeq:WP_014325558.1", "UniRef:UniRef100_A0AAX0SPT1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SPT1", "uniref90_id": "UniRef90_P75313" }, "psc": { "uniref90_id": "UniRef90_P75313", "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "ec_ids": [ "3.4.11.9" ], "uniref50_id": "UniRef50_P47566", "cog_id": "COG0006", "cog_category": "E", "go_ids": [ "GO:0004177", "GO:0046872", "GO:0008237", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47566", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47566" ], "product": "Putative Xaa-Pro aminopeptidase" }, "genes": [ "pepP" ], "id": "GIHJHMOALN_1013", "locus": "GCA009939745_01009" }, { "type": "cds", "sequence": "contig_1", "start": 569015, "stop": 569896, "strand": "-", "frame": 3, "gene": "degV", "product": "DegV domain-containing MG326-like protein", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "RefSeq:WP_010874828.1", "SO:0001217", "UniParc:UPI0000139625", "UniRef:UniRef100_P75312", "UniRef:UniRef50_P75312", "UniRef:UniRef90_P75312" ], "nt": "ATGAAGACCGCGATAATTACTGACTCGACTGCCTCAATTAAGGAAGGAGAGATCCAAGACGTTTACGTGTTACCTTTACAGGTCATTATTAACGGTCAAGACACTTACCGCGATGGTAAGGACATTGACTATGATCGAGTATACCAGTTGTTGAAGGAACACCCCCAAGGGTTAAACATTTCCACATCACTACCACGCCAAGCTGACTTAATTGAGTTAATTGAAGCCATTAAAGACAAGTACGATCGCTTTGTTTTTTTACCTCTCAGTAAGGGTTTGAGTGGTACGTATGACATGATTGTCCAAGCGGTTAAGCCACTCAGTACGCCCAAAAAAGAGTTTGTGGTCTTGGAAACAAGTGACATTGCCATTTCCCTGAAATGGTTAGTACAAGAGGTGAAGGCATTAACTGACACTAACTGCCCAACTAAAGCAATTGCTGAAGTGGTTGATCAGCACAAACAAAGTATTTTTACAGCTGTGACTGTAAAAAACTTAGTGCAGCTACGTAAAGGTGGGCGCATTTCTGGATTAAAGAAAATTATTGCCACACTTTTACGGGTTAAACCGATTATCTTCTTTGATAAAGGGGTAAATACTTTGAGCGGCAAGGCCTTTACCTTTGTCCAAGCATTGGAGAAAATCTTTACCTTTGTCAAATCAAAATTTGGTGATAACTTTAAGATCAAACGGATTGGTTTTTGCAATGCTTTTACCCCAGTTAAGGCCAAGGAAGTTAAAGCTTTAATTTTGGATTTTCTCCACACTAACAAAATCACTTTACAACGTGAAATCGAAAGCTCGTTTATCACAAGTGCCATTATTGCGCATACCGGAATAGATGCGTTTTCGATCAGTTTATTACTCGATAAAAATAAATAA", "aa": "MKTAIITDSTASIKEGEIQDVYVLPLQVIINGQDTYRDGKDIDYDRVYQLLKEHPQGLNISTSLPRQADLIELIEAIKDKYDRFVFLPLSKGLSGTYDMIVQAVKPLSTPKKEFVVLETSDIAISLKWLVQEVKALTDTNCPTKAIAEVVDQHKQSIFTAVTVKNLVQLRKGGRISGLKKIIATLLRVKPIIFFDKGVNTLSGKAFTFVQALEKIFTFVKSKFGDNFKIKRIGFCNAFTPVKAKEVKALILDFLHTNKITLQREIESSFITSAIIAHTGIDAFSISLLLDKNK", "aa_hexdigest": "46556300cca56294085e003b00954d5f", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI0000139625", "ncbi_nrp_id": "WP_010874828.1", "uniref100_id": "UniRef100_P75312", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139625", "RefSeq:WP_010874828.1", "UniRef:UniRef100_P75312" ] }, "ips": { "uniref100_id": "UniRef100_P75312", "uniref90_id": "UniRef90_P75312" }, "psc": { "uniref90_id": "UniRef90_P75312", "gene": "DegV", "product": "DegV domain-containing protein MG326 homolog", "uniref50_id": "UniRef50_P75312", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P75312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75312" ], "product": "DegV domain-containing protein MG326 homolog" }, "genes": [ "degV" ], "id": "GIHJHMOALN_1014", "locus": "GCA009939745_01010" }, { "type": "cds", "sequence": "contig_1", "start": 569902, "stop": 570708, "strand": "-", "frame": 1, "gene": "menH", "product": "Putative esterase/lipase 2", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0016020", "GO:0052689", "RefSeq:WP_010874829.1", "SO:0001217", "UniParc:UPI000012A1B6", "UniRef:UniRef100_P75311", "UniRef:UniRef50_P75311", "UniRef:UniRef90_P75311" ], "nt": "ATGGCAAGCAGTAAGAGCGATCCCTTTCAATCAATCTTTGCCTTTAAACCACACCGTAAGCGCCATAACTTTATCTTTCTCCATGGTTTTGGCAGTGAGTATTCCAGCTTTAAACACGTGTTTAAACTGTTTGAAAAAAAGCGCTGGTCGTTCTTTGCCTTTAATTTTCCGGGACACGGCAATAATCAGAGTAACAGTGTTGATGAATTGAAGTTGAAACACTATGTTGAATTGGTGTGTGACTTTATCATCCAAAAGCGCTTGAAAAAGGTGGTATTGGTAGGGCACAGTATGGGCGGGGCGATTGCGGTGTTGGTCAATGCTGTATTGCGTGAACGCATTAAGGCCTTAGTGTTGGTAGCACCGATGAATCAAACATCGTTTGTTGTAAGTAAAAAACGGATCTTGGATACCTTGTTTACCAGGAGTCCTAAAAACCAGCAGGACTTCATAGAACACACCGATGATAAGAAGTCGATTGTTAACTTCTTTGTTGGTGCTTTTAAGAAGCGAGTCAACTTTAAAACACTTTACAGTGACATGGTGCAAAACGCCAAGTATGGTAATGATTATCTCGAAGCGGGTTACAACGCAATTAAGGACAAACCCACCTTAGTGGTTCTCGGTTCCAATGATATTGTCACCCCCACAAAGGCTTCTGTGGAATACTTAGCTAAACACAGCGAAACGATCATTTTCAAAATTATTGATGGTGTAGGACATTCACCCCACTACTATGCACCCAAACTTTTTTTTGATTACATTGGGGAGTTTCTAGATAATATAAAGCGAAATAAGGACAAATAG", "aa": "MASSKSDPFQSIFAFKPHRKRHNFIFLHGFGSEYSSFKHVFKLFEKKRWSFFAFNFPGHGNNQSNSVDELKLKHYVELVCDFIIQKRLKKVVLVGHSMGGAIAVLVNAVLRERIKALVLVAPMNQTSFVVSKKRILDTLFTRSPKNQQDFIEHTDDKKSIVNFFVGAFKKRVNFKTLYSDMVQNAKYGNDYLEAGYNAIKDKPTLVVLGSNDIVTPTKASVEYLAKHSETIIFKIIDGVGHSPHYYAPKLFFDYIGEFLDNIKRNKDK", "aa_hexdigest": "77a8a3ac89beaa0abf5b2a128db2a15a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012A1B6", "ncbi_nrp_id": "WP_010874829.1", "uniref100_id": "UniRef100_P75311", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012A1B6", "RefSeq:WP_010874829.1", "UniRef:UniRef100_P75311" ] }, "ips": { "uniref100_id": "UniRef100_P75311", "uniref90_id": "UniRef90_P75311" }, "psc": { "uniref90_id": "UniRef90_P75311", "gene": "MenH", "product": "Putative esterase/lipase 2", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75311", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0016020", "GO:0052689" ] }, "pscc": { "uniref50_id": "UniRef50_P75311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75311" ], "product": "Putative esterase/lipase 2" }, "genes": [ "menH" ], "id": "GIHJHMOALN_1015", "locus": "GCA009939745_01011" }, { "type": "cds", "sequence": "contig_1", "start": 570695, "stop": 574117, "strand": "-", "frame": 3, "gene": "smc", "product": "putative MG328-like protein", "db_xrefs": [ "COG:COG1196", "COG:D", "RefSeq:WP_014574996.1", "SO:0001217", "UniParc:UPI0001DD63FC", "UniRef:UniRef100_A0AAX0S3B7", "UniRef:UniRef50_P75310", "UniRef:UniRef90_P75310" ], "nt": "ATGACAGCAGAAAAAGAGCTAGGTATCCACGATTTTGACGAAAACTTAAACGAACAAAGTTTGCTCAAGCAACTCATGCCCAAAACCCGTAACATTACCCTAGAACCAACAAGGGAAACAATAACAGTTCCCCTAGAACGTAACATTGTAGAACGTAGTTTCTTTGGCGAATTAGATCAGTTAGAGAAAGAACAATCAATTCGCAATTTTTTTCACTTTATGGTAGATATGGGTGTGGTCAAAAGCCAACCCCATGTTGATGTGTTAAACCACTTCGCTAACCAACTCCAAGTTCATAAACCGCAAACCGTTGATCTAACAATGGAGTTTTTAGAACAAGAAGGCCAGGAAGTACTAACAAAGGAAATTAAGGCTGGTTTTTGTGAAATTACCCCCTCTAGTATTACCGAACAAACGACCAAGCCCCAGTTAGATGAAACTCAACTAGTCGATGAATACGTGCACACCAAAGAGTTAGAAACAACACCAATACCTATATCCTTTGCCACTAAGGAAGTACTGTTTGAAGAGGTCTTTAATACTCCTTCAACCCAACAAGTCGATGAAAGTGTGCTTGTCAACGAATACATTGAACTAACCCAACAAATCAAAAACGCATCAGAACAAGTTTCATCAAACCATACCCATCAGTTTAGTGTGGCAACAGAACCAGCAGCCACAAAAGCTGTTAGTGAAACTATGTTGTTGGATGACTATGTCGAAATGGTGGAACAGGATGTTCAAGCCCAAACTGCTTTACCCCAAGCAGCACTTGATCCTACTGTTAGCTTGACCTTTAGCAGTCCGATTGACAGTAATGCCATTTTGGTGTACCCGGAAATGAAGGTACCTCATGTATTTGACACAGTTGCACCAACTACCACAACTGTTCCGTTAGACCAAACACAACTGCTTGACGAACTAGTGGAAGTGCCAGTTTTAACTCACACTGTAACTCCAGCACCGTTACAACCAAAAGCAGCACCAACTAACTTTGCACTTGACCAAACCCAGTTAGTCGATGAATTAGTAACAGTACCACTCACCCATACATTAGTCAACGAGTCTGCACCAGTTACACCTGTTGTTGTGACATCACCTGCAGCTGAACATAGCTTCTCGATTACAACGGTGGACAAGGCTAATTTAACTAATGCTTTGAGCCAAACAGTGGTTATAAAACCCGCTGAAGACAGCGCTCATCAAAGTGCCGTTTTAGACAAGGAAATTGCCACCAAACAAGTGCAGTTACAACAATTGCAAGCCCAAATTGAACTACGCCAAGCTCAACTAGAAACACCTCCTGTAACTTACATGGGTGTGGAAGAATACAAGCTACTGCCTGTTCAAGATGTAGTGCCAGTACAACCTACCGTGAGTTTTGAAATGACCCTGCTCCAAGAACAGTTAGATAAGGCCTTGAAGCACAACGCTGCTTTACAAATCCAGCTAGAAGAGCAGTTAGCAAAGCCACTTCAGTACGACCAAAGTCCGGTGTTACAGGAACGGATTGAACTGTTGCAAAACCAAAATACTAACCTCACTCAGGAGTTGAATGAACTGCAACAAAAGCTGTTTAAGAGCCAAAACAACAGCTTACTCTTAGCACGTTTGGAAGAGGAAAACCGTACGCTTAAACAGCATTTACAAAACAACCTACCGGAAGCTAACCAGTTAAACTTTGTCTTGGAAAAACAGCTTGAACAACTCCAACAAGACAAACACAGCTTAACACTCCAAATCGAACAGTACAAGTTTGATTCCAAAAAACACCAGGAACAACTGGCCTTAATTCCGTCGTTACGCAGTGAAATTAACAGTTTGGAAACGGAAGTAATTAGCTTAAAGCAAACTAACCAACGCTTAAGCCTAATTGAGCGTGAAAACAACTTCCTCAAAACCGAAATTAAACAGCTACGTGAAACTAAGTTAAACGATGAAAACACCAAGTACCGTAATCTTTTAAAGCAGTATGAGTTAATGCGTGCTGACAGTGATGCTAAGTTAAAGGAATTGGAACACGAACAACACCTAGCACACCAACACCACCAGGAACAGTTAGCACAATTACAACGCCACAACGAAGCTTTAGTAAAAGAGTTAGACCAAGTTAAGGCCACTAACTTTGAGTTAGGTCTCGCAGCACAAGGTTTTGAACAGCAAAAAGTGGTACTAGAACAAAAAAACAGCAGTTTACTTGCAAGCTTACAAGCAGCTGAGGAAAACGTCCATGCCTTGGGTATTACTAACAGTGAACTACAAAACCAACTAAATGTTTTAGAATTTACCCACAAGGAAAAAACTGCGTTTGACAGCAAAACGCTAACACTCACAAAGCAACAGCTAGAACAAACCCAGTTTGACCTTAGCTTAACCCAAGAACAGTTAGCTACCTTCAAACAACAAAACCAAAGCCTAACTGACAAACTAATGGCTAGCGAAACCCAGTTAAACCACTTGCAACAGAGTGATGAAAACTTAACCCAGCTCCAAACGCAGCACGAGCTGTTGCAAGAGAGTTACAACAAATTGCAAGATGAAGCTAATCACACCCAGCAACAATTCCATCAAGCACAAAATGAACTTGATGCTGCCCACCAACAGTTAGCACTCTTTAAGCAAAACAACGAGGAATTAACTGATAAATGCAGTAACATTCAAAATGAGTTGCATGATTTAAACCGGGTAAAGACTAACTGGGAGAACCTCAATACAGAGCATAACTTGTTGCAAGACAAATATGCTCAACAAAAAGAGCAAATGCAACATGAACACAGTAATTTAGCCCAAATCCAAGCGGAGCATGAGTTACTGCAAGAGAGTTACAACAAGGTCAAAGCAGAATTAAACGAAATTCAAATTACTAACCTTAATGAGGCCAACGCCCAGTACCAGGACTTGCTCTCCGCTTACGAGTTGTTACAAAGTAATCACAACAAGCTCAAGCAAGAGTTGCAAGTTTTAAACCAGGTCAATTTGGAAAAACAACAGTTAGCGCAAAAGTTACACAACACACACCAAAGTTTAAGTCAAACCCATGCAGAGCTAACCCAACTCCAAGCTGCTTACAATAACCTCCAAGCAACTCCACCGGTAAGCGATGAATTGTTAGAACAATTTAACCAAGTGCAGCTAGAAAAACAACGCTTACTGCAACAAAACTTAGCCTTAGTACACGAGTTGCAATACTTCAACGAACTCAACTCGAGTCAAACCCACGAAATTAAAACAAAGCAAGACGAAACTGTTAAAGAGGTCATTATTGTGGAAAAGGAAATTCCAGTGCTACCCGAAAAGAAACCACGGCTAAAAAAACGTGATATTGTGATCGAAAACAAGGAAGATGCTTTGGGCAAGTTATCCAAAAAGGAACGGATCCAAGCGTACGCTGAACGCCTAGCTAAGATCAATGGCAAGCAGTAA", "aa": "MTAEKELGIHDFDENLNEQSLLKQLMPKTRNITLEPTRETITVPLERNIVERSFFGELDQLEKEQSIRNFFHFMVDMGVVKSQPHVDVLNHFANQLQVHKPQTVDLTMEFLEQEGQEVLTKEIKAGFCEITPSSITEQTTKPQLDETQLVDEYVHTKELETTPIPISFATKEVLFEEVFNTPSTQQVDESVLVNEYIELTQQIKNASEQVSSNHTHQFSVATEPAATKAVSETMLLDDYVEMVEQDVQAQTALPQAALDPTVSLTFSSPIDSNAILVYPEMKVPHVFDTVAPTTTTVPLDQTQLLDELVEVPVLTHTVTPAPLQPKAAPTNFALDQTQLVDELVTVPLTHTLVNESAPVTPVVVTSPAAEHSFSITTVDKANLTNALSQTVVIKPAEDSAHQSAVLDKEIATKQVQLQQLQAQIELRQAQLETPPVTYMGVEEYKLLPVQDVVPVQPTVSFEMTLLQEQLDKALKHNAALQIQLEEQLAKPLQYDQSPVLQERIELLQNQNTNLTQELNELQQKLFKSQNNSLLLARLEEENRTLKQHLQNNLPEANQLNFVLEKQLEQLQQDKHSLTLQIEQYKFDSKKHQEQLALIPSLRSEINSLETEVISLKQTNQRLSLIERENNFLKTEIKQLRETKLNDENTKYRNLLKQYELMRADSDAKLKELEHEQHLAHQHHQEQLAQLQRHNEALVKELDQVKATNFELGLAAQGFEQQKVVLEQKNSSLLASLQAAEENVHALGITNSELQNQLNVLEFTHKEKTAFDSKTLTLTKQQLEQTQFDLSLTQEQLATFKQQNQSLTDKLMASETQLNHLQQSDENLTQLQTQHELLQESYNKLQDEANHTQQQFHQAQNELDAAHQQLALFKQNNEELTDKCSNIQNELHDLNRVKTNWENLNTEHNLLQDKYAQQKEQMQHEHSNLAQIQAEHELLQESYNKVKAELNEIQITNLNEANAQYQDLLSAYELLQSNHNKLKQELQVLNQVNLEKQQLAQKLHNTHQSLSQTHAELTQLQAAYNNLQATPPVSDELLEQFNQVQLEKQRLLQQNLALVHELQYFNELNSSQTHEIKTKQDETVKEVIIVEKEIPVLPEKKPRLKKRDIVIENKEDALGKLSKKERIQAYAERLAKINGKQ", "aa_hexdigest": "315cdd9ace9c2b83a954a0d547d1a3f2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD63FC", "ncbi_nrp_id": "WP_014574996.1", "uniref100_id": "UniRef100_A0AAX0S3B7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD63FC", "RefSeq:WP_014574996.1", "UniRef:UniRef100_A0AAX0S3B7" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S3B7", "uniref90_id": "UniRef90_P75310" }, "psc": { "uniref90_id": "UniRef90_P75310", "gene": "Smc", "product": "Uncharacterized protein MG328 homolog", "uniref50_id": "UniRef50_P75310", "cog_id": "COG1196", "cog_category": "D" }, "pscc": { "uniref50_id": "UniRef50_P75310", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75310" ], "product": "Uncharacterized protein MG328 homolog" }, "genes": [ "smc" ], "id": "GIHJHMOALN_1016", "locus": "GCA009939745_01012" }, { "type": "cds", "sequence": "contig_1", "start": 574154, "stop": 574576, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14714.11" ], "nt": "ATGGCTAAGTTTAAAAAGCAGTTACAAAGTCAGTTTCAACACCTCAGCTTTGCTCCCATTGTTTTTATTAGTGTCAAAAACAACAAGCGCTTACATACCATCTTTGAACAGCTCCAAATTATCCAGGAACAGCTCACCAAAAAGATTTCAACTTCCCTGTTAAACGATGTCATCCAACAAGCACAACTGTTTAACCAAGCACCCCTGTTTAAGGGTGGGAGGTTACAGGTAACTTATGCGGTGCAAACTCACAGTCAAACACCGCACTTTGTACTGTTCTGTAATGACCCTAAGTTTGTCCACTTTTCTTATGCGCGCTTTTTAGAGAACAAGATTAGGGAAAGCTTTGGTTTTAGTGCTGTACCTATTACTCTTTATTTCAAGTCCAAAAATGCACGGATAAGAGGTGTCGCAAAAACCTAA", "aa": "MAKFKKQLQSQFQHLSFAPIVFISVKNNKRLHTIFEQLQIIQEQLTKKISTSLLNDVIQQAQLFNQAPLFKGGRLQVTYAVQTHSQTPHFVLFCNDPKFVHFSYARFLENKIRESFGFSAVPITLYFKSKNARIRGVAKT", "aa_hexdigest": "baa0285819b27dedec0c8312a740e54d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14714.11", "name": "KH_dom-like", "length": 81, "aa_cov": 0.5785714285714286, "hmm_cov": 1.0, "evalue": 3.5728950157000198e-31, "score": 102.84827423095703, "start": 48, "stop": 128 } ], "seq_stats": { "molecular_weight": 16233.708699999988, "isoelectric_point": 10.332235145568848 }, "id": "GIHJHMOALN_1017", "locus": "GCA009939745_01013" }, { "type": "cds", "sequence": "contig_1", "start": 574604, "stop": 575503, "strand": "-", "frame": 3, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "SO:0001217", "UniRef:UniRef50_P75309", "UniRef:UniRef90_P75309" ], "nt": "GTGTTTACTGTTGCCATTATCGGCCGACCTAACGTTGGCAAATCGAGTTTGTTCAATCGCTTAATCCAAAAACCCTACGCGATTATTTCCGATACGCCCAATACCACAAGGGACCGCATTTATGGTGTTGGTGAATGGTTAACGCGGCAAATTGCCTTTATTGATACTGGGGGATTAATTTCCCAAAAGACCCCGCTCCAACAACAAATTGAAGTCCAAGTTCGAGCAGCCTTAAGTCAAGCTAATGCGATTATCTTCTTGGTGTCTTACCAAGAACAGATTAGCAGTGATGACTTTTATGTAGCTAAGGTATTGAAAAAGATTAAGGACAAACCGATTTTGTTGGTCGTCAATAAATCAGAAAACCTCAAACCAGATGCTTATGAACCCAACCTACAACAGTTCTACAGCTTTGGGTTTGGCCAACCCGTTTGTGTGAGTGCTAGCCATGGCATTGGCATTGGTAACCTAATGGATCGACTAGTTAAGGACAACCAACTCCCCCCTTACCATGGTAGTAGTGAAACCAATCCCGAAGTGCGCTTTTGTGTAATTGGTAAACCCAATGTTGGCAAGAGTTCTTTAATTAACCAACTGGTGCAGCAAAACCGGGTGTTAGTGTCCGATGAGAGTGGTACTACCCGTGATGCAATTGATATTCCCTTAAGGGTTAATGGGCAAAACTACCTTTTAATTGATACGGCTGGGATTCGCCGTAAGGGGAAAATAGCCCCCGGAATTGAAGCGGCCTCGTATGGGAAAACCCAGTTAGCAATCGCTAGGAGCAACATTATTCTGTTAATGGTTGATGGTTCCAAACCGTTGAGTGAACAGGACGAGATTATCGGTGGATTAGCCCAAGCCGCTTTAATTCCGGTGATTATTCTGGTCAACAAATGA", "aa": "MFTVAIIGRPNVGKSSLFNRLIQKPYAIISDTPNTTRDRIYGVGEWLTRQIAFIDTGGLISQKTPLQQQIEVQVRAALSQANAIIFLVSYQEQISSDDFYVAKVLKKIKDKPILLVVNKSENLKPDAYEPNLQQFYSFGFGQPVCVSASHGIGIGNLMDRLVKDNQLPPYHGSSETNPEVRFCVIGKPNVGKSSLINQLVQQNRVLVSDESGTTRDAIDIPLRVNGQNYLLIDTAGIRRKGKIAPGIEAASYGKTQLAIARSNIILLMVDGSKPLSEQDEIIGGLAQAALIPVIILVNK", "aa_hexdigest": "5ea8883a405ca73f4a52e9ab9e63f8cc", "start_type": "GTG", "rbs_motif": null, "genes": [ "der" ], "truncated": "5-prime", "pseudogene": { "start": 574301, "stop": 575503, "observations": { "mutation": [ 574606 ], "stop": [ 574606 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75309", "query_cov": 1.0, "subject_cov": 0.6659242761692651, "identity": 1.0, "score": 582.0, "evalue": 1.92e-207, "gene_start": 1, "gene_end": 299, "reference_start": 1, "reference_end": 299, "reference_sequence": "MFTVAIIGRPNVGKSSLFNRLIQKPYAIISDTPNTTRDRIYGVGEWLTRQIAFIDTGGLISQKTPLQQQIEVQVRAALSQANAIIFLVSYQEQISSDDFYVAKVLKKIKDKPILLVVNKSENLKPDAYEPNLQQFYSFGFGQPVCVSASHGIGIGNLMDRLVKDNQLPPYHGSSETNPEVRFCVIGKPNVGKSSLINQLVQQNRVLVSDESGTTRDAIDIPLRVNGQNYLLIDTAGIRRKGKIAPGIEAASYGKTQLAIARSNIILLMVDGSKPLSEQDEIIGGLAQAALIPVIILVNKWDLVQKDSNTMAKFKKQLQSQFQHLSFAPIVFISVKNNKRLHTIFEQLQIIQEQLTKKISTSLLNDVIQQAQLFNQAPLFKGGRLQVTYAVQTHSQTPHFVLFCNDPKFVHFSYARFLENKIRESFGFSAVPITLYFKSKNARIRGVAKT" }, "paralog": false, "identity": 0.995, "score": 773.0, "evalue": 4.3e-285, "description": "Internal stop codon at 574606. Nonsense mutation around 574606.", "psc": { "uniref90_id": "UniRef90_P75309", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75309", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75309", "EC:3.6.5.-", "UniRef:UniRef50_P75309", "COG:COG1160", "COG:J", "KEGG:K03977", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P75309", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75309" ], "product": "GTPase Der" } }, "id": "GIHJHMOALN_1018", "locus": "GCA009939745_01014" }, { "type": "cds", "sequence": "contig_1", "start": 575505, "stop": 576158, "strand": "-", "frame": 2, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "COG:COG0283", "COG:F", "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431", "RefSeq:WP_010874832.1", "SO:0001217", "UniParc:UPI000012DCF0", "UniRef:UniRef100_P75308", "UniRef:UniRef50_P47572", "UniRef:UniRef90_P75308" ], "nt": "ATGTATTTTCAAATTGCCATTGACGGTCCTAGTAGTTCTGGTAAATCTAGTGTTGCTAAAACAGTGGCCCGACAGCTCGGTTTTGAGTACTTTTCCACTGGCAAAATGTACCGGGCCTTCGCTTACGTGATGCAGGTCAACCGCTTGGATGTCAACCTTTTGTTAAAGGTGATTAACCAAATTAACTGGCGCTTTGAACACGAAAAGGTGTTTTATAACAACGCTGATATCTCAGAGGTTATCCTAAACCAGGAAATTGCCCAACTAGCGAGCAATTTAGCAACCAATCCCGAAGTGCGTAAGATGGCGGTATTGCGCCAACAAGCGTTAGCTAAAAACACCAACATTGTGATGGATGGACGCGACATTGGTACCGTTGTATTGAAGGACGCGCAGTTAAAGTACTTCTTGGATGCTAAACCGGAAATAAGAGCGCAACGACGCGCCCAAGATCTTGGCATAGCATATGACAGTGACAAGGCCTTTCAGGAACTAGTAGCGGAAATTAAGCACCGTGATGCGGTGGATACTTCCAGAACTGCTGATCCCTTAGTGCAAGCCCCCGATGCGATTTACATCGACAGTTCTAACCTTACTTTTCAGCAAGTAGTCGAGTTAATGGTGCAACAAGCGCGTACAGTTTTTAAACTTTAA", "aa": "MYFQIAIDGPSSSGKSSVAKTVARQLGFEYFSTGKMYRAFAYVMQVNRLDVNLLLKVINQINWRFEHEKVFYNNADISEVILNQEIAQLASNLATNPEVRKMAVLRQQALAKNTNIVMDGRDIGTVVLKDAQLKYFLDAKPEIRAQRRAQDLGIAYDSDKAFQELVAEIKHRDAVDTSRTADPLVQAPDAIYIDSSNLTFQQVVELMVQQARTVFKL", "aa_hexdigest": "2ab2763f60287f23f9a9edc9ce80afdf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012DCF0", "ncbi_nrp_id": "WP_010874832.1", "uniref100_id": "UniRef100_P75308", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012DCF0", "RefSeq:WP_010874832.1", "UniRef:UniRef100_P75308" ] }, "ips": { "uniref100_id": "UniRef100_P75308", "uniref90_id": "UniRef90_P75308" }, "psc": { "uniref90_id": "UniRef90_P75308", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_P47572", "cog_id": "COG0283", "cog_category": "F", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ] }, "pscc": { "uniref50_id": "UniRef50_P47572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47572" ], "product": "Cytidylate kinase" }, "genes": [ "cmk" ], "id": "GIHJHMOALN_1019", "locus": "GCA009939745_01015" }, { "type": "cds", "sequence": "contig_1", "start": 576185, "stop": 576694, "strand": "-", "frame": 3, "gene": null, "product": "putative MG331-like protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75307", "UniRef:UniRef90_P75307" ], "nt": "TTGAGCCCGAAAATTCTGTCGCACTACCAGCCTTTTAGTGAATTGAGTGCTAATCCGGTGAAACGCAAAAGTGAACCCAATCAGGTCTTACAATCACTGCAAACTCTCATTAACAACTTCCAAACATCGGATTTTCAAGCCATCGCCACCCAAGCTGATGCCTTGTGGTTACAAATTAAACACCACGATAGCGCGACTGGTTATGAAAGTTGGATTACTGATGACCGGGGGATGGATAAAATAGATAACCTAAAGCGCTTTTTTGAGGACAACGAACAACAGTTTATTCAGCAAACGCAAACTTTCGTAGTGCAATTGGGGAACTATAATAAGGCACTAATGGCACTCACTAACGAAAAAGTGAGCTTTACCACCACTGATTTAAGCCAGCCCTTAGCGAGCAGTTACAACCCGTTATTTAAGCGGATTTTGCTCAGTTTATTAGTATTCTTTATTAGTAGTTTAGTGGGTTTGGTTGTTTTACTAACCCTCTTATTCACTTCCCTTTAA", "aa": "MSPKILSHYQPFSELSANPVKRKSEPNQVLQSLQTLINNFQTSDFQAIATQADALWLQIKHHDSATGYESWITDDRGMDKIDNLKRFFEDNEQQFIQQTQTFVVQLGNYNKALMALTNEKVSFTTTDLSQPLASSYNPLFKRILLSLLVFFISSLVGLVVLLTLLFTSL", "aa_hexdigest": "b5b40653b8a922273dadba5fbd55fb84", "start_type": "TTG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_P75307", "query_cov": 1.0, "subject_cov": 0.8086124401913876, "identity": 0.9940000000000001, "score": 315.0, "evalue": 9.49e-108, "valid": true, "product": "Uncharacterized protein MG331 homolog", "uniref50_id": "UniRef50_P75307", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P75307", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75307" ], "product": "Uncharacterized protein MG331 homolog" }, "genes": [], "id": "GIHJHMOALN_1020", "locus": "GCA009939745_01016" }, { "type": "cds", "sequence": "contig_1", "start": 576713, "stop": 576814, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACGCTTAGAGAAGTTTCGCTTTTACCGCCAGAACGATGACAATAATACGACTGTTCAAAAAGCGTTACTGAAAGCGCAAAAACAGGTCGCTAGTTGA", "aa": "MGRLEKFRFYRQNDDNNTTVQKALLKAQKQVAS", "aa_hexdigest": "cd0a4e426ba0cf40f9ec792e15d2b977", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3899.396700000001, "isoelectric_point": 10.160878181457516 }, "id": "GIHJHMOALN_1021", "locus": "GCA009939745_01017" }, { "type": "cds", "sequence": "contig_1", "start": 576817, "stop": 577524, "strand": "-", "frame": 1, "gene": "tACO1", "product": "putative transcriptional regulatory protein MPN_478", "db_xrefs": [ "COG:COG0217", "COG:KJ", "GO:0003677", "GO:0005829", "GO:0006355", "RefSeq:WP_010874834.1", "SO:0001217", "UniParc:UPI000013963C", "UniRef:UniRef100_P75306", "UniRef:UniRef50_P47574", "UniRef:UniRef90_P75306" ], "nt": "ATGCCGCGGAAGCATTTAATTGCCAGTCAAACAAACAAAAAACAACAATCCAACGCGAAGCAACTACAAAAGTTAGCGAAGCGGATCGCTGCTGCTGTCAAAAAAGGTGGCAGTAACATTGATGCCAACCCCCAGCTAAAGGTGGCAGTCGAACTAGCGTTAGCTCATGGTTTAAGTGCGGATTCAATTAAACGCAACATCCATGGCAGTGAAAAGGATCCCACAAAACTAAGCGAGTTTTGCTATGAGATCTTTGGTCCCAATGGTGTTGGCATCATTGTCTTTGGTCTAACCGATAATCCGAACCGGTTGTTGAGTAGTTTAAACGGTTACATTGCTAAATTAAAGGCACAGCTAGCCAAGCCCAACAGTGTTAAAATTAACTTTGAGGAAAAGGGCATTGCGCTAGTTAAACACAATAACTTTACCCAAGACGAGTTAATTGAACTGTTAATTAGTAACAACATTAATTTGTTAGATCTCAATGAAGATGATGACAGCTTTGAAGTAGTGGTCGATTCACCGAGTTACTTTGCGTTGAAGGATTTACTCGTTAAAAACAGTTTTACGATTGAAGCGAGTGAGTTGCGCTTAATTCCGCTGTTAACGGTGGAACTTAACGCCGAACAACACACCCTCTTAAACCGCTTTTTAAATGCGTGTGAAGAGGATGATGACATCCAAACAGTAGTGCATAATGCACTGTAG", "aa": "MPRKHLIASQTNKKQQSNAKQLQKLAKRIAAAVKKGGSNIDANPQLKVAVELALAHGLSADSIKRNIHGSEKDPTKLSEFCYEIFGPNGVGIIVFGLTDNPNRLLSSLNGYIAKLKAQLAKPNSVKINFEEKGIALVKHNNFTQDELIELLISNNINLLDLNEDDDSFEVVVDSPSYFALKDLLVKNSFTIEASELRLIPLLTVELNAEQHTLLNRFLNACEEDDDIQTVVHNAL", "aa_hexdigest": "94cd58cb630adeb32042c4dea2725291", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013963C", "ncbi_nrp_id": "WP_010874834.1", "uniref100_id": "UniRef100_P75306", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013963C", "RefSeq:WP_010874834.1", "UniRef:UniRef100_P75306" ] }, "ips": { "uniref100_id": "UniRef100_P75306", "uniref90_id": "UniRef90_P75306" }, "psc": { "uniref90_id": "UniRef90_P75306", "gene": "TACO1", "product": "Probable transcriptional regulatory protein MPN_478", "uniref50_id": "UniRef50_P47574", "cog_id": "COG0217", "cog_category": "KJ", "go_ids": [ "GO:0005829", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47574", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47574" ], "product": "Probable transcriptional regulatory protein MG332" }, "genes": [ "tACO1" ], "id": "GIHJHMOALN_1022", "locus": "GCA009939745_01018" }, { "type": "cds", "sequence": "contig_1", "start": 577702, "stop": 578121, "strand": "-", "frame": 1, "gene": "azoR", "product": "FMN-dependent NADH:quinone oxidoreductase", "db_xrefs": [ "COG:C", "COG:COG1182", "EC:1.6.5.-", "GO:0009055", "GO:0010181", "GO:0016652", "GO:0016655", "KEGG:K01118", "SO:0001217", "UniRef:UniRef50_P47575", "UniRef:UniRef90_P75305" ], "nt": "ATGAAGAAGGTAATTATTATTGATGCTTCTGTATCACCTAGTGGTTCTTACACCCATTTATTGTTAGAACGCTTTTTAGCTACCTTTCAAGCTAAAAACAAGGACGTAGAGCTCTCCACTTGGAACCTCAACGAACTACCAGTGGGACAAATTTCCTATAACACCCAAAACGCTGGAACTTTCTTTTCTGTAGAAAACTCCGATAAGTACATTGATGCGCTCAAAGCAGCGCACGGTGTTATTATCTTGGCGCCAATGACCAACTTTAACTACCCCGCAACGTTAAAGAACTTTATTGACCATGTCTTTGTGGCAAACAAAACCTTTAAGGATAAGTATGTCACTAAGGGCGCATCATCAGGAATGTTGGGTAACCTCAAGGTAGTCGTTTTGGGTTCCCAAGGCGCACCGTTAGGTTGA", "aa": "MKKVIIIDASVSPSGSYTHLLLERFLATFQAKNKDVELSTWNLNELPVGQISYNTQNAGTFFSVENSDKYIDALKAAHGVIILAPMTNFNYPATLKNFIDHVFVANKTFKDKYVTKGASSGMLGNLKVVVLGSQGAPLG", "aa_hexdigest": "c15fd1d27fece49966a775d820ee2fcf", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "azoR" ], "truncated": "5-prime", "pseudogene": { "start": 577528, "stop": 578121, "observations": { "mutation": [ 577704 ], "stop": [ 577704 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75305", "query_cov": 1.0, "subject_cov": 0.7055837563451777, "identity": 1.0, "score": 275.0, "evalue": 6.42e-93, "gene_start": 1, "gene_end": 139, "reference_start": 1, "reference_end": 139, "reference_sequence": "MKKVIIIDASVSPSGSYTHLLLERFLATFQAKNKDVELSTWNLNELPVGQISYNTQNAGTFFSVENSDKYIDALKAAHGVIILAPMTNFNYPATLKNFIDHVFVANKTFKDKYVTKGASSGMLGNLKVVVLGSQGAPLGWYPWGDHVNSLRGLFGFAGVASFASVIIDGTKLLYKDKSKSEVVDMFAKQVDAIANEF" }, "paralog": false, "identity": 0.9949238578680203, "score": 388.0, "evalue": 9.89e-140, "description": "Internal stop codon at 577704. Nonsense mutation around 577704.", "psc": { "uniref90_id": "UniRef90_P75305", "gene": "azoR", "product": "FMN-dependent NADH:quinone oxidoreductase", "ec_ids": [ "1.6.5.-" ], "uniref50_id": "UniRef50_P47575", "cog_id": "COG1182", "cog_category": "C", "kegg_orthology_id": "K01118", "go_ids": [ "GO:0009055", "GO:0010181", "GO:0016652", "GO:0016655" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75305", "EC:1.6.5.-", "UniRef:UniRef50_P47575", "COG:COG1182", "COG:C", "KEGG:K01118", "GO:0009055", "GO:0010181", "GO:0016652", "GO:0016655" ] }, "pscc": { "uniref50_id": "UniRef50_P47575", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47575" ], "product": "FMN-dependent NADH:quinone oxidoreductase" } }, "id": "GIHJHMOALN_1023", "locus": "GCA009939745_01019" }, { "type": "cds", "sequence": "contig_1", "start": 578149, "stop": 578667, "strand": "-", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6", "UniRef:UniRef90_UPI001EFE51D6" ], "nt": "ATGCTAAGTACCGTTACTCCCTTCTTGGCGGAACGGATCTACCAACAGTTTCACAGTGGCAGTGTCATGCAAGCAAGTTGGCCAACTGCCAAAGCAGTTAAACCACCGAAACTGTTTGCTGATGTTGTTGAAGCCGTGTCCAGCTTGCGCCACTACAAGGCCAACAACCAACTAGTAGCTAACCAGAATTTAACCGTTGTTTTAAGTGGTAAAGCTGCTCCTGTAGTGCAAAACTACTTCCACTTTAACTGGGTCGATCTTAGGATAGAAGTTAACAAAACTCCAGGCTTCCAAATCAAAATCGTTGACAATGCAGCCAACAACTTAGCCCACTTAGAAAAACAACGGAGCTTTTATTTAGCGGAAGTCCAACGTAGTCAAGCGATTAACACTAACCCTGCCTTTCTAAAAAAAGCACCACCGCACAAAGTTAAGGCGGAGCTTTTAAAGTTAGAGGAATATCAAAAAAAATTAGCGGAAGTCAATCATTTAATCGCTAAATTAACTAAAGCTGAGTAG", "aa": "MLSTVTPFLAERIYQQFHSGSVMQASWPTAKAVKPPKLFADVVEAVSSLRHYKANNQLVANQNLTVVLSGKAAPVVQNYFHFNWVDLRIEVNKTPGFQIKIVDNAANNLAHLEKQRSFYLAEVQRSQAINTNPAFLKKAPPHKVKAELLKLEEYQKKLAEVNHLIAKLTKAE", "aa_hexdigest": "7a8e46fe17aab007175f7e64626681e5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 578149, "stop": 578967, "observations": { "mutation": [ 578775 ], "stop": [ 578775 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFE51D6", "query_cov": 1.0, "subject_cov": 0.46112600536193027, "identity": 0.988, "score": 332.0, "evalue": 5.55e-112, "gene_start": 1, "gene_end": 172, "reference_start": 202, "reference_end": 373, "reference_sequence": "MPTDLLVTGYDILFFWVLRMTFNSYFQTKQLPFKQVLIHGLVRDAQNRKMSKSLNNGINPMDLIRDYGADATRLFLTSNHTPGDDLIFNEQKLKSAANFLNKLWNVTKYVLQLGEQAKTVPSTHLPSTLSERWIWAKLKQLIVQTTKLLDKYQLALANQALVNFIWNDFCNTFIETIKQEDTALLPQLYTTAKTVLSTAVVMLSTVTPFLAERIYQQFHSGSVMQASWPTAKAVKPPKLFADVVEAVSSLRHYKANNQLVANQNLAVVLSGKAAPVVQNYFHFNWVDLRIEVNKTPGFQIKIVDNAANNLAHLEKQRSFYLAEVQRSQAITTNPAFLKKAPPHKVKAELLKLEEYQKKLAEVNHLIAKLTKAE" }, "paralog": false, "identity": 0.9889705882352942, "score": 529.0, "evalue": 5.82e-192, "description": "Internal stop codon at 578775. Nonsense mutation around 578775.", "psc": { "uniref90_id": "UniRef90_UPI001EFE51D6", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFE51D6", "UniRef:UniRef50_UPI001EFE51D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6" ], "product": "class I tRNA ligase family protein" } }, "id": "GIHJHMOALN_1024", "locus": "GCA009939745_01020" }, { "type": "cds", "sequence": "contig_1", "start": 578773, "stop": 579270, "strand": "-", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6", "UniRef:UniRef90_UPI001EFE51D6" ], "nt": "GTGCCAACTGATCTTTTGGTAACCGGGTATGACATCCTCTTCTTTTGGGTCTTACGGATGACCTTTAACTCGTACTTTCAAACCAAGCAGTTACCTTTTAAACAAGTGTTAATTCACGGTCTAGTCCGCGATGCCCAAAACCGCAAAATGTCCAAGTCCCTAAACAACGGGATTAACCCAATGGACTTAATCCGTGATTATGGGGCCGATGCCACGCGCTTATTCTTAACTTCCAATCACACTCCGGGTGATGATTTAATCTTCAACGAACAGAAGTTAAAGAGTGCCGCTAACTTCTTGAACAAGTTGTGGAATGTGACCAAGTACGTGTTGCAGTTAGGAGAACAAGCCAAAACTGTACCAAGTACTCACTTACCCTCTACATTGAGTGAGCGCTGGATCTGGGCTAAATTGAAACAACTCATTGTCCAAACCACCAAGTTATTAGACAAGTACCAGTTAGCTTTGGCCAACCAAGCGTTAGTCAACTTTATTTGA", "aa": "MPTDLLVTGYDILFFWVLRMTFNSYFQTKQLPFKQVLIHGLVRDAQNRKMSKSLNNGINPMDLIRDYGADATRLFLTSNHTPGDDLIFNEQKLKSAANFLNKLWNVTKYVLQLGEQAKTVPSTHLPSTLSERWIWAKLKQLIVQTTKLLDKYQLALANQALVNFI", "aa_hexdigest": "0247efda14e376f468612d986773bbb0", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 578473, "stop": 579270, "observations": { "mutation": [ 578775 ], "stop": [ 578775 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFE51D6", "query_cov": 1.0, "subject_cov": 0.44235924932975873, "identity": 1.0, "score": 331.0, "evalue": 5.92e-112, "gene_start": 1, "gene_end": 165, "reference_start": 1, "reference_end": 165, "reference_sequence": "MPTDLLVTGYDILFFWVLRMTFNSYFQTKQLPFKQVLIHGLVRDAQNRKMSKSLNNGINPMDLIRDYGADATRLFLTSNHTPGDDLIFNEQKLKSAANFLNKLWNVTKYVLQLGEQAKTVPSTHLPSTLSERWIWAKLKQLIVQTTKLLDKYQLALANQALVNFIWNDFCNTFIETIKQEDTALLPQLYTTAKTVLSTAVVMLSTVTPFLAERIYQQFHSGSVMQASWPTAKAVKPPKLFADVVEAVSSLRHYKANNQLVANQNLAVVLSGKAAPVVQNYFHFNWVDLRIEVNKTPGFQIKIVDNAANNLAHLEKQRSFYLAEVQRSQAITTNPAFLKKAPPHKVKAELLKLEEYQKKLAEVNHLIAKLTKAE" }, "paralog": false, "identity": 0.9924528301886792, "score": 523.0, "evalue": 5.98e-190, "description": "Internal stop codon at 578775. Nonsense mutation around 578775.", "psc": { "uniref90_id": "UniRef90_UPI001EFE51D6", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFE51D6", "UniRef:UniRef50_UPI001EFE51D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFE51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFE51D6" ], "product": "class I tRNA ligase family protein" } }, "id": "GIHJHMOALN_1025", "locus": "GCA009939745_01021" }, { "type": "cds", "sequence": "contig_1", "start": 579421, "stop": 580179, "strand": "-", "frame": 1, "gene": "valS", "product": "valine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.9", "GO:0000166", "GO:0004832", "GO:0005524", "GO:0006438", "SO:0001217", "UniRef:UniRef50_UPI001EFEBE54", "UniRef:UniRef90_UPI001EFEBE54" ], "nt": "TTGTTTGAACAGGGCTTTATCTACCAAGCTGAAACCTTGGTTAACTGGGACACCAAGCTAAACACTGCCATCTCGAACATTGAAGTCATTAATAAACCAGTTGACCAACAGCTGTATTACATTGCTTATAAGTTAGCTAATAATCCCAAAAAACGTTTAGTGGTGGCAACTACTAGACCAGAAACGATCTTTGTTGATGTGTGTTTGTTTGTCCATCCCAAAGACAAGCACTACCACAGCTTTGTCAAACAAAAGGTAGTTAATCCGCTCACTGGTGCGTTAATGCCAGTGTTCACCGATAGTTATGTCGACAAGAAGTTTGGCACCGGTGTGTTGAAGTGCACCCCCGCCCATGACTTTAACGACTTTGCCTTAAACGAAAAGTACCGTTTACCGTTTGTAAGTTGCATTGACCACAATGGTTTGTTAAATGAGCACGCTAAGCAATTTACTGGTTTAACCGTAAGTGCAGCACGTCAGCAGGTAGTCGAGTTTTTACAAACGCAAAAACTACTAGTGAAAACTATGCCACTAACCAGTAACGTTGGTTTTTCCGAGCGCAGTGACACGGTCGTAGAACCGTTATTATCCAAGCAGTGGTTTGTCGATTTACCGAAACTCAAAAAAGCACTCGCCATTAAGAAATACCCCGAGTTAATTCCCAAGCGTTTTAACAAACAGGTAACAAGGTGGTTGAGTCAGCTCAAACCGTGGTGCATATCGCGCCAATTAATTTGGGGTCACCCTATTCCGGTGTGA", "aa": "MFEQGFIYQAETLVNWDTKLNTAISNIEVINKPVDQQLYYIAYKLANNPKKRLVVATTRPETIFVDVCLFVHPKDKHYHSFVKQKVVNPLTGALMPVFTDSYVDKKFGTGVLKCTPAHDFNDFALNEKYRLPFVSCIDHNGLLNEHAKQFTGLTVSAARQQVVEFLQTQKLLVKTMPLTSNVGFSERSDTVVEPLLSKQWFVDLPKLKKALAIKKYPELIPKRFNKQVTRWLSQLKPWCISRQLIWGHPIPV", "aa_hexdigest": "9f1bbed346d36710fa071d13504163d2", "start_type": "TTG", "rbs_motif": null, "genes": [ "valS" ], "truncated": "both", "pseudogene": { "start": 579203, "stop": 580479, "observations": { "deletion": [ 579348 ], "mutation": [ 579423, 580245 ], "stop": [ 579240, 579261, 579423, 580245 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFEBE54", "query_cov": 1.0, "subject_cov": 0.5163934426229508, "identity": 0.996, "score": 517.0, "evalue": 5.77e-182, "gene_start": 1, "gene_end": 252, "reference_start": 163, "reference_end": 414, "reference_sequence": "MDKQFSFQGQYDFKTVSTGLYDSWSSASFFKPQKNKVPFTAILPPPNLTGTLHIGHAFEVSITDQIMRFKRLRGYGVNWIPGFDHAGIATQTKYEKLARETNPEYFQAPRKQKVKMIMDWALTQGDTIQSQIKSLGASLNWNQVNFTLSKKASQIVNDSFIQLFEQGFIYQAETLVNWDTKLNTAISNIEVINKPVDQQLYYIAYKLANNPKKRLVVATTRPETIFVDVCLFVHPKDKHYHSFVKQKVVNPLTGALMPVFTDSYVDKKFGTGVLKCTPAHDFNDFALNEKYRLPFVSCIDHNGLLNEHAKQFTGLTVSAARQQVVEFLQTQKLLVKTMPLTSNVGFSERSDTVVEPLLSKQWFVDLPKLKKALAIKKYPELIPKRFNKQVTRWLSQLKPWCISRQLIWGHPIPVWTHKQSGALHVGSTAPTDKQNYTQSXHRCTRYLIFLITVTVNLFRLTQKQALCANWSFGNRVWHPLLLGLTDDL" }, "paralog": false, "identity": 0.9859154929577465, "score": 839.0, "evalue": 1.84e-311, "description": "Internal stop codon at 579240, 579261, 579423, 580245. Frameshift due to deletion around 579348. Nonsense mutation around 579423, 580245.", "psc": { "uniref90_id": "UniRef90_UPI001EFEBE54", "gene": "valS", "product": "valine--tRNA ligase", "ec_ids": [ "6.1.1.9" ], "uniref50_id": "UniRef50_UPI001EFEBE54", "go_ids": [ "GO:0000166", "GO:0004832", "GO:0005524", "GO:0006438" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFEBE54", "EC:6.1.1.9", "UniRef:UniRef50_UPI001EFEBE54", "GO:0000166", "GO:0004832", "GO:0005524", "GO:0006438" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFEBE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFEBE54" ], "product": "valine--tRNA ligase" } }, "id": "GIHJHMOALN_1026", "locus": "GCA009939745_01022" }, { "type": "cds", "sequence": "contig_1", "start": 580243, "stop": 580488, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGAGTATCACTGACCAAATTATGCGCTTTAAGCGACTGCGGGGTTATGGCGTGAACTGGATTCCCGGATTTGACCATGCGGGGATTGCTACGCAAACCAAATACGAAAAGTTAGCACGGGAAACTAATCCCGAGTACTTCCAAGCGCCGCGTAAACAAAAGGTCAAAATGATTATGGATTGGGCCTTAACACAAGGGGACACCATTCAAAGTCAGCTCAAAAGTTTAGGGGCATCCTTAAACTGA", "aa": "MSITDQIMRFKRLRGYGVNWIPGFDHAGIATQTKYEKLARETNPEYFQAPRKQKVKMIMDWALTQGDTIQSQLKSLGASLN", "aa_hexdigest": "833acd389589ce28da4f8483f2d2daf6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 80, "aa_cov": 0.9753086419753086, "hmm_cov": 1.0, "evalue": 1.1214507307361907e-14, "score": 49.437538146972656, "start": 2, "stop": 80 } ], "seq_stats": { "molecular_weight": 9319.644399999996, "isoelectric_point": 9.81829319000244 }, "id": "GIHJHMOALN_1027", "locus": "GCA009939745_01023" }, { "type": "cds", "sequence": "contig_1", "start": 580649, "stop": 581230, "strand": "-", "frame": 3, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872", "RefSeq:WP_010874837.1", "SO:0001217", "UniParc:UPI000003226E", "UniRef:UniRef100_P75303", "UniRef:UniRef50_P47577", "UniRef:UniRef90_P75303" ], "nt": "ATGGAGGCACGTTTTTTAAAAAGTGCAAGTGACTTAGAGAGTTGTCCCCAAGACAGTGTTAAGGAAGTCTGTTTTATGGGGCGGAGTAATGTCGGCAAATCAAGCCTCATTAATGCCTTTTTTCAAAAAAAGCTAGCTAAAACTTCCGCGACGCCGGGACGTACACAACTGTTAAACTTCTTTGAATACAACCGTAAACGCTTTGTCGATTTACCAGGTTATGGCTTTGCTAAACTGAGTAAAGTGCAAAAGGAAGCAATTACTAATTTATTAACGCAGTTCCTCAATTTTCGCCAAAACTTAACTGGTGTGGTACTAGTGATTGACAGCGGTGTAGTGACCGTTCAGGACCAGGAAGTGGTCAAAACCATTCTCCAAACGGGGTTAAATTTTTTGGTGATAGCCAATAAGTTTGATAAGCTCAACCAAAGCGAGCGCTTTCATACCCAGAATAAACTGGCACACTTTTTAAAAGTCAATCCTAATAAGTGTTTATTTGTTAGTGCCAAAACCGGGTATAACCTCCAAGTAATGCACAAACAAATCTTTGAGTTATTTAAAGCAGATGGACAAGCAATTTAG", "aa": "MEARFLKSASDLESCPQDSVKEVCFMGRSNVGKSSLINAFFQKKLAKTSATPGRTQLLNFFEYNRKRFVDLPGYGFAKLSKVQKEAITNLLTQFLNFRQNLTGVVLVIDSGVVTVQDQEVVKTILQTGLNFLVIANKFDKLNQSERFHTQNKLAHFLKVNPNKCLFVSAKTGYNLQVMHKQIFELFKADGQAI", "aa_hexdigest": "abd56841e4c3a5c5f3ceb4780214e371", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000003226E", "ncbi_nrp_id": "WP_010874837.1", "uniref100_id": "UniRef100_P75303", "db_xrefs": [ "SO:0001217", "UniParc:UPI000003226E", "RefSeq:WP_010874837.1", "UniRef:UniRef100_P75303" ] }, "ips": { "uniref100_id": "UniRef100_P75303", "uniref90_id": "UniRef90_P75303" }, "psc": { "uniref90_id": "UniRef90_P75303", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_P47577", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47577", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47577" ], "product": "Probable GTP-binding protein EngB" }, "genes": [ "yihA" ], "id": "GIHJHMOALN_1028", "locus": "GCA009939745_01024" }, { "type": "cds", "sequence": "contig_1", "start": 581238, "stop": 581459, "strand": "-", "frame": 2, "gene": "yneF", "product": "UPF0154 MG335-1-like protein", "db_xrefs": [ "COG:COG3763", "COG:S", "GO:0016020", "KEGG:K09976", "RefSeq:WP_014574997.1", "SO:0001217", "UniParc:UPI00001833B6", "UniRef:UniRef100_Q9EXD7", "UniRef:UniRef50_Q9EXD7", "UniRef:UniRef90_Q9EXD7" ], "nt": "ATGAATGATCTTGCGCTTGCCTTAGGTTTGGGAATTCCCCTTTCCCTGTTAGTCGGTGTCATTATTGGCTATTTTATTTCTATCAAAATCTTTAAAAAACAGATTCGTGATAACCCACCAATCACCGAAAACCAAATTAAGGCGATGTATGCCAAAATGGGTCGTAAGCTTTCTGAAACACAAGTCAAGGAAATTATGCGCTCCATTAAAAACCAGAAGTAA", "aa": "MNDLALALGLGIPLSLLVGVIIGYFISIKIFKKQIRDNPPITENQIKAMYAKMGRKLSETQVKEIMRSIKNQK", "aa_hexdigest": "6b8518efc9d5c23459413584c515b25c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001833B6", "ncbi_nrp_id": "WP_014574997.1", "uniref100_id": "UniRef100_Q9EXD7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001833B6", "RefSeq:WP_014574997.1", "UniRef:UniRef100_Q9EXD7" ] }, "ips": { "uniref100_id": "UniRef100_Q9EXD7", "uniref90_id": "UniRef90_Q9EXD7" }, "psc": { "uniref90_id": "UniRef90_Q9EXD7", "gene": "YneF", "product": "UPF0154 protein MG335.1 homolog", "uniref50_id": "UniRef50_Q9EXD7", "cog_id": "COG3763", "cog_category": "S", "kegg_orthology_id": "K09976", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD7" ], "product": "UPF0154 protein MG335.1 homolog" }, "genes": [ "yneF" ], "id": "GIHJHMOALN_1029", "locus": "GCA009939745_01025" }, { "type": "cds", "sequence": "contig_1", "start": 581518, "stop": 582147, "strand": "+", "frame": 1, "gene": "wcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.1.-", "GO:0005886", "GO:0006071", "GO:0046467", "GO:0046509", "KEGG:K19004", "SO:0001217", "UniRef:UniRef50_Q9ZB73", "UniRef:UniRef90_P75302" ], "nt": "ATGAACAAGCTAATTAGCATTCTGGTTCCTTGTTACCAATCCCAGCCCTTTCTCGATCGCTTTTTCAAATCACTTTTAAAGCAAGACTGGAACGGTGTTAAAGTAATCTTCTTTAATGATAACAAGCCAGATCCAACATACGAGATCCTAAAACAATTTCAACAAGCACATCCACAACTAGCGATTGAAGTACATTGTGGTGAGAAAAATGTCGGTGTAGGCGGTTCACGTGATCAACTAATAAATTATGTAGACACACCCTACTTTTACTTTGTTGATCCTGATGATGAATTTAGTGATCCCAACTGTTTTAAGGCAATAGTGGAAACAATTCAAGGAGAAAACTTTGATATTGCAGTGTTAAACAGTATTGTTTACCTCCAAATGTTGAAGAATGACTTTTTAATTAAACACATTCCGCTCAAAAACATCTTTCAGGGCAAGGTAAAACTCAATCCTGATAACACCGTAAATCATTTGCACTATATCCAGAATAACGACCAGTACATTTGGAACATTGTCATCAACACCGCCTTTTTCAAAGCATTGGATTTGCAGTTTGTTAACCGCTTTATTGAAGACATTGCGGTGTGGTTTCCGATTATGTTTAAAGCCCAAAAAGTGCTGTGA", "aa": "MNKLISILVPCYQSQPFLDRFFKSLLKQDWNGVKVIFFNDNKPDPTYEILKQFQQAHPQLAIEVHCGEKNVGVGGSRDQLINYVDTPYFYFVDPDDEFSDPNCFKAIVETIQGENFDIAVLNSIVYLQMLKNDFLIKHIPLKNIFQGKVKLNPDNTVNHLHYIQNNDQYIWNIVINTAFFKALDLQFVNRFIEDIAVWFPIMFKAQKVL", "aa_hexdigest": "116bcb9caa314d6839faa26b1fee38b6", "start_type": "ATG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 581518, "stop": 582450, "observations": { "mutation": [ 582145, 582322 ], "stop": [ 582145, 582322 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75302", "query_cov": 1.0, "subject_cov": 0.6129032258064516, "identity": 1.0, "score": 436.0, "evalue": 7.44e-153, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MNKLISILVPCYQSQPFLDRFFKSLLKQDWNGVKVIFFNDNKPDPTYEILKQFQQAHPQLAIEVHCGEKNVGVGGSRDQLINYVDTPYFYFVDPDDEFSDPNCFKAIVETIQGENFDIAVLNSIVYLQMLKNDFLIKHIPLKNIFQGKVKLNPDNTVNHLHYIQNNDQYIWNIVINTAFFKALDLQFVNRFIEDIAVWFPIMFKAQKVLWIDVNGVNYYLRPNSASTQKNSIKLLSFIEAYERLYFHLKKVGKLADFIDPNNKIESRFWRRQAFIWFSFINVSWMKAEFEQTKSVLQKLFDFMEANGIYDRVFTNKHHGIYLLWVNRLKHFKKLVQAQPHL" }, "paralog": false, "identity": 0.9935483870967742, "score": 631.0, "evalue": 3.48e-232, "description": "Internal stop codon at 582145, 582322. Nonsense mutation around 582145, 582322.", "psc": { "uniref90_id": "UniRef90_P75302", "gene": "WcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "ec_ids": [ "2.4.1.-" ], "uniref50_id": "UniRef50_Q9ZB73", "cog_id": "COG0463", "cog_category": "M", "kegg_orthology_id": "K19004", "go_ids": [ "GO:0005886", "GO:0046509", "GO:0006071", "GO:0046467" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75302", "EC:2.4.1.-", "UniRef:UniRef50_Q9ZB73", "COG:COG0463", "COG:M", "KEGG:K19004", "GO:0005886", "GO:0046509", "GO:0006071", "GO:0046467" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB73" ], "product": "Processive diacylglycerol beta-glycosyltransferase" } }, "id": "GIHJHMOALN_1030", "locus": "GCA009939745_01026" }, { "type": "cds", "sequence": "contig_1", "start": 582175, "stop": 582324, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGACCGAACAGTGCTTCCACCCAGAAAAACAGCATTAAATTACTCTCCTTTATTGAGGCTTACGAACGGTTGTATTTCCACTTAAAGAAGGTGGGTAAGCTAGCTGATTTTATTGACCCTAACAATAAGATTGAAAGTCGTTTTTGA", "aa": "MRPNSASTQKNSIKLLSFIEAYERLYFHLKKVGKLADFIDPNNKIESRF", "aa_hexdigest": "9997683b229596b41da09e2cef7b4904", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5759.5947, "isoelectric_point": 9.77445468902588 }, "id": "GIHJHMOALN_1031", "locus": "GCA009939745_01027" }, { "type": "cds", "sequence": "contig_1", "start": 582370, "stop": 582489, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCGGAATTTGAACAGACCAAAAGTGTGTTGCAAAAACTGTTTGACTTCATGGAAGCCAACGGCATCTACGACAGGGTATTTACTAATAAGCACCACGGTATTTACTTATTGTGA", "aa": "MKAEFEQTKSVLQKLFDFMEANGIYDRVFTNKHHGIYLL", "aa_hexdigest": "a6aa4d2c1b90fa3fa9ade9edace266a8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4663.333899999999, "isoelectric_point": 6.704352378845214 }, "id": "GIHJHMOALN_1032", "locus": "GCA009939745_01028" }, { "type": "cds", "sequence": "contig_1", "start": 582554, "stop": 582850, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_017532763.1", "SO:0001217", "UniParc:UPI0002F4AA32", "UniRef:UniRef100_UPI0002F4AA32", "UniRef:UniRef50_A0A0H3DKQ0", "UniRef:UniRef90_A0A0H3DKQ0" ], "nt": "TTGGCAACCGAGTTGCAAGTGGTAGCTATTTTTTCAATCTCCAAAAAACAAATATTAATTATGAAAAATAAAAAAATTATCAAACTACCTTTTATTGATCACGCAATGAATTCACTCAATCAAAGTGAGCTAATTTCATTTGTAGCTTTTAACGAAGCCAATGAAGATAATAAACGCACACTTTACATAAAGATTCACGCTAATCAAAAGGAAATGGTTGTACAAGCCCAAGATGGAAAACAAACTCTAGAAAGAGAACAACTTTTTAAAAAGCTACTCGAAGCTTTAGCTTGTTAG", "aa": "MATELQVVAIFSISKKQILIMKNKKIIKLPFIDHAMNSLNQSELISFVAFNEANEDNKRTLYIKIHANQKEMVVQAQDGKQTLEREQLFKKLLEALAC", "aa_hexdigest": "9836dd356fafd039539fa67574de5c51", "start_type": "TTG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0002F4AA32", "ncbi_nrp_id": "WP_017532763.1", "uniref100_id": "UniRef100_UPI0002F4AA32", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F4AA32", "RefSeq:WP_017532763.1", "UniRef:UniRef100_UPI0002F4AA32" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002F4AA32", "uniref90_id": "UniRef90_A0A0H3DKQ0" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DKQ0", "uniref50_id": "UniRef50_A0A0H3DKQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DKQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DKQ0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11276.158399999993, "isoelectric_point": 9.098954963684083 }, "id": "GIHJHMOALN_1033", "locus": "GCA009939745_01029" }, { "type": "cds", "sequence": "contig_1", "start": 583048, "stop": 583359, "strand": "-", "frame": 1, "gene": null, "product": "UPF0134 protein MPN_484", "db_xrefs": [ "RefSeq:WP_010874840.1", "SO:0001217", "UniParc:UPI000013AB4F", "UniRef:UniRef100_P75301", "UniRef:UniRef50_P75076", "UniRef:UniRef90_P75301" ], "nt": "ATGGGCTTTTATGGCAATTTAAACCATATGGAAAAACGAAAAAGTGGTTACGTTACCCAAAAGCAGTTTAATGATTTCAAAAATTCCAACAATCAGCGGCTGATAAAAATTGAGAACACTTTGGTTTCTCAAGGTGAACAAATTAGTCAGCTAATCAAAGTTTCAATTCTTCAGGGTGAACAAATAAATAAGCTCACTGAAACTGTTGAAAAGCAGGGCGAACAAATTCAAACCCAAGGGGAAACGCTTAAGTTAATTCTTGAAACTCTTCAAGTAATTAACAAACGTTTGGATCGTTTGGAGTCTAAGTAG", "aa": "MGFYGNLNHMEKRKSGYVTQKQFNDFKNSNNQRLIKIENTLVSQGEQISQLIKVSILQGEQINKLTETVEKQGEQIQTQGETLKLILETLQVINKRLDRLESK", "aa_hexdigest": "9af49134a83744b70dcf9c7cd697c5d8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AB4F", "ncbi_nrp_id": "WP_010874840.1", "uniref100_id": "UniRef100_P75301", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AB4F", "RefSeq:WP_010874840.1", "UniRef:UniRef100_P75301" ] }, "ips": { "uniref100_id": "UniRef100_P75301", "uniref90_id": "UniRef90_P75301" }, "psc": { "uniref90_id": "UniRef90_P75301", "product": "UPF0134 protein MPN_484", "uniref50_id": "UniRef50_P75076" }, "pscc": { "uniref50_id": "UniRef50_P75076", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75076" ], "product": "UPF0134 protein MPN_038" }, "genes": [], "id": "GIHJHMOALN_1034", "locus": "GCA009939745_01030" }, { "type": "cds", "sequence": "contig_1", "start": 583776, "stop": 584072, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTCGGGTCAGGGGACCCAAGTTAAGGGCAGTGTGCAGGGGAGTAGCCGAGCAGCTTCTGTTAGCGTTCAAACTACCCAGCAAAGCAGACAGCAATCGACTGATACCCAAGAATCGGAAGTGGTTAAGTTAGCCAAAGCGCTGTTGAAATCAAGTGCGGATTTGGCCAAACCCTTCACTGATAACCCCACCTTTAAAAAGGCCTTAACCGACATCCAATCCGAGTACAAGGATTATTTGGCTGCTGCGGGGAAGTTAAGTGAGTTTAAGAAAGATTTGGGGGAGGTGAGTTAA", "aa": "MGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKALLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAGKLSEFKKDLGEVS", "aa_hexdigest": "7acd74ec4be84e3f0a1153eba477fb91", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10415.452099999991, "isoelectric_point": 8.925663948059086 }, "id": "GIHJHMOALN_1035", "locus": "GCA009939745_01031" }, { "type": "cds", "sequence": "contig_1", "start": 584121, "stop": 584888, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A8A20B3", "UniRef:UniRef90_UPI000A8A20B3" ], "nt": "GTGACCAAGTCCAACCTGCGCGGGGCCTTTAAGGGCGTGGGGTTAGCTACGGCCATTATTGACCAGTACGAGTACCTGGTGGGTGGGAGTAAGACGAGTTCGTTGCCCGAGGTCAAGGTAGATTCGAATAAGTCCAACCAAAATCCGCTGGATAGTTTTTTTATGGAGGGGAAGGATGCCGTGGCGATTAGATCCATAGTTTCGCGTGCGAAAATTGCCATGACCGACCAAACCCCCGGGTTTAAGGTCGACCCGGCGTTTGTCAAGGTGAAGCAGAATAATCAAACCGATGCGTTTTACACAACCCAGCGCAAACTGAGCGGCGGGCAAACTAATGGGGGTAGTAATAATTCCAATGATAAACACCACTACCTCCAAGACGCGGTGCGGTTGACGAGTAGTCAAGCAATGGCGGCAGCTTCCACTGGGGCAGATTCTAGTAGTGGTACCAATGTTGGTGGTAGCTCCGGGGGCAACTCTGTTTTAATTCCGCTCCCAAGGAGTGCCGCCCTTACCCACACCCAGCAACAAGTTCAACAAACCACCTCCACACTCCAAACCCCTGTTTACGCCCGTGGTGACGATGGTACATACGCCCTGGCAATTGACGGTGGTGATTATTTTTTGGCGAACAACAAACGCGATTTCACCAAACAAGCCGACATCTTGCTGTACCGCTATTTACAAGCTAAGAGTAATAACTTTAAGGAAAACGGCGTCGAGTTTAGTTTGAACTTACTGGAATCTGGCAGCCTGTTCCAAACGTGA", "aa": "MTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVDPAFVKVKQNNQTDAFYTTQRKLSGGQTNGGSNNSNDKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQT", "aa_hexdigest": "5c7a14f2b9f6e46b102105e77875d2cf", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 583818, "stop": 585002, "observations": { "mutation": [ 584123, 584921 ], "stop": [ 584123, 584921 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A8A20B3", "query_cov": 1.0, "subject_cov": 0.625, "identity": 0.996, "score": 403.0, "evalue": 4.03e-138, "gene_start": 1, "gene_end": 255, "reference_start": 39, "reference_end": 293, "reference_sequence": "MFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVDPAFVKVKQNNQTDAFYTTQRKLSGGQTNGGSNNSNDKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQTWAQTGLTAKLYGALVAMMGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKALLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAGKLSEFKKDLGEVS" }, "paralog": false, "identity": 0.9898477157360406, "score": 620.0, "evalue": 3.18e-226, "description": "Internal stop codon at 584123, 584921. Nonsense mutation around 584123, 584921.", "psc": { "uniref90_id": "UniRef90_UPI000A8A20B3", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI000A8A20B3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A8A20B3", "UniRef:UniRef50_UPI000A8A20B3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A8A20B3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A8A20B3" ], "product": "DUF3713 domain-containing protein" } }, "id": "GIHJHMOALN_1036", "locus": "GCA009939745_01032" }, { "type": "cds", "sequence": "contig_1", "start": 584919, "stop": 585221, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE", "UniRef:UniRef90_UPI0006B4FEFE" ], "nt": "TTGCTCTATCTGTTTCCCCTCACCCAAGCGTTCTTCGCGTACCAAGCACCCAAGGACGGGCTCGACAGCTTATACGACCAAGCCGCGATTGGTAGTGCCTTACAGTTGGTCTATGCCTTCCCCGCGTTTCGCGAGCCCAATAATGGACAAAGCCAAGGTAAAACCACCTTCGACCCCACCCCCAACAGTGCCCAAAACTTCGGGGATTTCATTAAGGCGGTGTTTCCGGAGCAGAAGAATGGTCAAACCCAACAATCCAATACATCCTCCCGCACCGGGTTGTTTGACTGGCAAACCAAGTGA", "aa": "MLYLFPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTK", "aa_hexdigest": "5f3381ebf7f1d74115b541bf5cae3c11", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 584690, "stop": 585221, "observations": { "insertion": [ 584732 ], "mutation": [ 584921 ], "stop": [ 584921 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "query_cov": 1.0, "subject_cov": 0.5681818181818182, "identity": 1.0, "score": 205.0, "evalue": 3.38e-66, "gene_start": 1, "gene_end": 100, "reference_start": 1, "reference_end": 100, "reference_sequence": "MLYLFPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFLWRGRMPWRLDP" }, "paralog": false, "identity": 0.9886363636363636, "score": 349.0, "evalue": 2.54e-125, "description": "Internal stop codon at 584921. Frameshift due to insertion around 584732. Nonsense mutation around 584921.", "psc": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0006B4FEFE", "UniRef:UniRef50_UPI0006B4FEFE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE" ], "product": "DUF3713 domain-containing protein" } }, "id": "GIHJHMOALN_1037", "locus": "GCA009939745_01033" }, { "type": "cds", "sequence": "contig_1", "start": 585315, "stop": 585698, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGACCAAAACTAAGTTTAATCCGTACCAATTTCGCAAACAATTTAAGTGGTTTAAAAACAACCCCCAATGGGTTAACTTTGACAACGCTGCTACCTCAATTGCGCTAGACACCGTTAGTCAAGCTTGCAAGGAATACTATGAGCTTTTTAGTGTCAACCCCCACAATAAAACCCCTGATCTCAACAACCAGATTATTGCCATTATTGCTGAAACCCGTCAACTAGTAGCGGACTGGTTTAATGTCACTGCACCTGAAATTATCTTTACAAGTTCAGCCACGGAGTCCATTAATTTGTTTGCCCATGGCTTAAAGCCCTGGATTAAACCCGGTGATGAAATTGTCTTAAAGGGTGATGAACACTCGGCTAACGTTTTGCCCTGA", "aa": "MTKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPDLNNQIIAIIAETRQLVADWFNVTAPEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDEHSANVLP", "aa_hexdigest": "9885f69ee1cc4c5f4ea2b762be9c8623", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 104, "aa_cov": 0.8110236220472441, "hmm_cov": 1.0, "evalue": 1.682610016527444e-31, "score": 104.9789810180664, "start": 25, "stop": 127 } ], "seq_stats": { "molecular_weight": 14529.281099999984, "isoelectric_point": 6.269079399108887 }, "id": "GIHJHMOALN_1038", "locus": "GCA009939745_01034" }, { "type": "cds", "sequence": "contig_1", "start": 585699, "stop": 586541, "strand": "+", "frame": 3, "gene": "csd", "product": "putative cysteine desulfurase", "db_xrefs": [ "COG:COG0520", "COG:E", "EC:2.8.1.7", "GO:0031071", "SO:0001217", "UniRef:UniRef50_Q49420", "UniRef:UniRef90_P75298" ], "nt": "GTGGCACTAGCTAAACAAACAAAGGCCAGACTAGTATGGGTCGAAAAGCAAGCTAACCAATCCTTAGAAGACACTTTTAAGTCATTGATTAACCCGAAAACTAAAGTAGTAGCCATTACCGCTACTTCCAATTTGTTTGGCAATTCAATTGACTTTGCTCAAATTGCCGATTACCTCAAACAAGTCAATCCTAAAGCTTTTGTAGCCGTGGATGCAGTGCAAACGGTGCAGCACAAGCAAATTGACATTGCCAAAACCCAGATCGACTTCCTAGCCTTTTCTACTCATAAGTTTTACGGTCCCACTGGACTGGGGGTAGCTTACATTAAAAAAAGCTTACAACCCCAATTACAACCCCTCAAACTCGGGGGTGATATCTTTACCCAGATTGACCCGGACAATACAATTCACTTTAAAAGTACACCCCTAAAGTTTGAAGCCGGTACCCCCAACATTATGGCCATCTATGCCTTAAACAAGCTGTTGCGCTTCTTTAAACAAAAGTTTAATTTTGCCCAAATGATGGCTTATGGTCACCAACTTAAACAACAAGCTTACGAACTGTTAAACAGTAATCCGCAAATTGTGCTGGCCAACCACGACCAGGATGTCCCGATTTTTTCCTTTAAACACCGCCAATTGGCTACCATTGATCTAGCCACTTTCCTAAACATCAATAAGATCATGGTACGCCAGGGCTCCATTTGTGTGGGACGTTACCGCAATAAGGATTACTTTGTGAGAGTTTCGTTAATGCACTATAACACAGTGAAGGAGTTGCAGTATTTAGCTAAACTCCTAGCAACTGATACCAAGACAATTATTAAAAACGTAATTAAGTAA", "aa": "MALAKQTKARLVWVEKQANQSLEDTFKSLINPKTKVVAITATSNLFGNSIDFAQIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKSLQPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQMMAYGHQLKQQAYELLNSNPQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQGSICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTKTIIKNVIK", "aa_hexdigest": "bbba49cb3e5110c6dc2b63919d160bbf", "start_type": "GTG", "rbs_motif": null, "genes": [ "csd" ], "truncated": "5-prime", "pseudogene": { "start": 585399, "stop": 586541, "observations": { "mutation": [ 585696 ], "stop": [ 585696 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75298", "query_cov": 1.0, "subject_cov": 0.6862745098039216, "identity": 0.996, "score": 548.0, "evalue": 3.58e-195, "gene_start": 1, "gene_end": 280, "reference_start": 129, "reference_end": 408, "reference_sequence": "MTKTKFNPYQFRKQFKWFKNNPQWVNFDNAATSIALDTVSQACKEYYELFSVNPHNKTPDLNNQIIAIIAETRQLVADWFNVTALEIIFTSSATESINLFAHGLKPWIKPGDEIVLKGDEHSANVLPWVALAKQTKARLVWVEKQANQSLEDTFKSLINPKTKVVAITATSNLFGNSIDFAQIADYLKQVNPKAFVAVDAVQTVQHKQIDIAKTQIDFLAFSTHKFYGPTGLGVAYIKKSLQPQLQPLKLGGDIFTQIDPDNTIHFKSTPLKFEAGTPNIMAIYALNKLLRFFKQKFNFAQMMAYGHQLKQQAYELLNSNPQIVLANHDQDVPIFSFKHRQLATIDLATFLNINKIMVRQGSICVGRYRNKDYFVRVSLMHYNTVKELQYLAKLLATDTKTIIKNVIK" }, "paralog": false, "identity": 0.9947368421052631, "score": 746.0, "evalue": 2.84e-275, "description": "Internal stop codon at 585696. Nonsense mutation around 585696.", "psc": { "uniref90_id": "UniRef90_P75298", "gene": "csd", "product": "Probable cysteine desulfurase", "ec_ids": [ "2.8.1.7" ], "uniref50_id": "UniRef50_Q49420", "cog_id": "COG0520", "cog_category": "E", "go_ids": [ "GO:0031071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75298", "EC:2.8.1.7", "UniRef:UniRef50_Q49420", "COG:COG0520", "COG:E", "GO:0031071" ] }, "pscc": { "uniref50_id": "UniRef50_Q49420", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49420" ], "product": "Probable cysteine desulfurase" } }, "id": "GIHJHMOALN_1039", "locus": "GCA009939745_01035" }, { "type": "cds", "sequence": "contig_1", "start": 586544, "stop": 586966, "strand": "+", "frame": 2, "gene": "iscU", "product": "putative MG337-like protein", "db_xrefs": [ "COG:COG0822", "COG:O", "GO:0005506", "GO:0016226", "GO:0051536", "RefSeq:WP_010874844.1", "SO:0001217", "UniParc:UPI0000139648", "UniRef:UniRef100_P75297", "UniRef:UniRef50_P75297", "UniRef:UniRef90_P75297" ], "nt": "ATGGATCGCAAAATAAGGGAGCAAATTATTGCCATTTACAGCAACTTACAACACAAACAGGACTTAACTCAGTTTGCCCATTGTTTAACCACTAAGGCTGATGATAACTGTGAGGACTTCTTTAACTTAGGCTTACAGTTTCAAAACAACCAACTAACAGCACTTGGTTTTAACGGGGAGGGTTGTATTATTTCGACAATTGCTAGTGAACTGACTTTAAGTGCGTTGGAAGGCAAAACAATCAAAGAAGCCATTGTCTTGTTAGAACAGTTTATGGAAGCTGTACAAACTGGTAGCTTAAGCACGCCACTACCCCAAGCTTTACAACTGTTGTGGGACTTTCAGATTGACCAGAAGCGTTTAAACTGCTTACTCCTAACACCACAGAATTTGCTACAGTGGCTTAAAGATTTTTCGCACTAA", "aa": "MDRKIREQIIAIYSNLQHKQDLTQFAHCLTTKADDNCEDFFNLGLQFQNNQLTALGFNGEGCIISTIASELTLSALEGKTIKEAIVLLEQFMEAVQTGSLSTPLPQALQLLWDFQIDQKRLNCLLLTPQNLLQWLKDFSH", "aa_hexdigest": "3a5136abf5d565a383c1df3dff163993", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI0000139648", "ncbi_nrp_id": "WP_010874844.1", "uniref100_id": "UniRef100_P75297", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139648", "RefSeq:WP_010874844.1", "UniRef:UniRef100_P75297" ] }, "ips": { "uniref100_id": "UniRef100_P75297", "uniref90_id": "UniRef90_P75297" }, "psc": { "uniref90_id": "UniRef90_P75297", "gene": "IscU", "product": "Uncharacterized protein MG337 homolog", "uniref50_id": "UniRef50_P75297", "cog_id": "COG0822", "cog_category": "O", "go_ids": [ "GO:0005506", "GO:0051536", "GO:0016226" ] }, "pscc": { "uniref50_id": "UniRef50_P75297", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75297" ], "product": "Uncharacterized protein MG337 homolog" }, "genes": [ "iscU" ], "id": "GIHJHMOALN_1040", "locus": "GCA009939745_01036" }, { "type": "cds", "sequence": "contig_1", "start": 586947, "stop": 587123, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005B22982", "UniRef:UniRef90_UPI0005B22982" ], "nt": "ATGGCTTTACTAGTAGAACAAGCCTTGGACGCTGGTACGCAAGAGTTAGCCTTTTTAGACTTAGTGCGCGCTAACCAGGTGCAAAACAAACGTAGTCACCAGCAAATCCTGGTGGGTGACAAACGCTTGTATGATGCGTTAGGGCAAACGCTAGCGCTTAGTGCGAAAAATCTTTAA", "aa": "MALLVEQALDAGTQELAFLDLVRANQVQNKRSHQQILVGDKRLYDALGQTLALSAKNL", "aa_hexdigest": "d3438a7ea1725d724407af1ebccd8493", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 586947, "stop": 587225, "observations": { "mutation": [ 587171 ], "stop": [ 587171 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005B22982", "query_cov": 1.0, "subject_cov": 0.6304347826086957, "identity": 1.0, "score": 109.0, "evalue": 3.72e-30, "gene_start": 1, "gene_end": 58, "reference_start": 35, "reference_end": 92, "reference_sequence": "MSYVIQITNEDVNKISASWKTDKNSALGLKKEIFMALLVEQALDAGTQELAFLDLVRANQVQNKRSHQQILVGDKRLYDALGQTLALSAKNL" }, "paralog": false, "identity": 0.9782608695652174, "score": 171.0, "evalue": 1.02e-56, "description": "Internal stop codon at 587171. Nonsense mutation around 587171.", "psc": { "uniref90_id": "UniRef90_UPI0005B22982", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI0005B22982", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005B22982", "UniRef:UniRef50_UPI0005B22982" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005B22982", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005B22982" ], "product": "DUF3713 domain-containing protein" } }, "id": "GIHJHMOALN_1041", "locus": "GCA009939745_01037" }, { "type": "cds", "sequence": "contig_1", "start": 587169, "stop": 587657, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCGACTGCTAGTGCCGGACTCACGGAACAAATCTTTAACAACTTTGGTCAGTTAGTAACAAGTAGTGACAAATCCGGGGCCTTGAGCCAGTACAAGGATAAAGCAACCCTGAAACGCTTAATCCAAAACACTAATTCCGATGCCGAGCTCAATGCCTTTGGTGAAGTGTTGCACCGTGCAGTTAATGTCGATACCAGTAACTTAGGACGTTTTAACTCCAGTGGGGAACCGTTGATTAGCTTTGACAACAAGAAGAAGTTCTTGGTTGATGTAGTGGACAAACTGGATGATGGTTACTTTAACAAGTTCGAAGGTTATGTTGGCCAAACCAAGGTGAAGATGTCTGATTCAAGTAGTAGTTCCCAAGGTAACGAAGACTATAAGAAAGCCAAAGCCGTACCACTCACCAAGGACTCGAGTGTCAAGACTTTGAGCTATGTCATTCAGATTACCAACGAGGACGTTAACAAAATTTCTGCTAGTTGA", "aa": "MSTASAGLTEQIFNNFGQLVTSSDKSGALSQYKDKATLKRLIQNTNSDAELNAFGEVLHRAVNVDTSNLGRFNSSGEPLISFDNKKKFLVDVVDKLDDGYFNKFEGYVGQTKVKMSDSSSSSQGNEDYKKAKAVPLTKDSSVKTLSYVIQITNEDVNKISAS", "aa_hexdigest": "ddb6b5ca80046837f5ec6f411d81cd2d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17679.421000000002, "isoelectric_point": 6.587207221984863 }, "id": "GIHJHMOALN_1042", "locus": "GCA009939745_01038" }, { "type": "cds", "sequence": "contig_1", "start": 587838, "stop": 587993, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTATGATGATACGGCTACGGCATCAGCAGCCGCAGCAAAAGCGCAAGTAGCAGTATTAAAGACAGCTCAGGCAACAAATACTCAAAGCGACAACCCCTTCAATAAGTTTGTCCAAAACCCCGATTATGTCCAGGGTTCGGAAACCAACTGA", "aa": "MSYDDTATASAAAAKAQVAVLKTAQATNTQSDNPFNKFVQNPDYVQGSETN", "aa_hexdigest": "345d1816f56bb0a4f4457f4db144b964", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5381.7197000000015, "isoelectric_point": 4.359459877014161 }, "id": "GIHJHMOALN_1043", "locus": "GCA009939745_01039" }, { "type": "cds", "sequence": "contig_1", "start": 588078, "stop": 588254, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCACTTTCAAGTAGCGCAGCAGTTGCAAATGCTGTAGCTTCAAGTGGCATGCAAAAATCTGGTGATCAAACCCTCTTGGAACTCGGTAAAAAAGCGTTTGAAAGTGAACTGGAAGCATCTAGTAGTGATGGGCAGTACAAGTACCTGCGCTTCTTGTCCACCTTAATGTGA", "aa": "MVSLSSSAAVANAVASSGMQKSGDQTLLELGKKAFESELEASSSDGQYKYLRFLSTLM", "aa_hexdigest": "b379422fe7e084317980c5e62fef166e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6121.832899999998, "isoelectric_point": 5.052213478088378 }, "id": "GIHJHMOALN_1044", "locus": "GCA009939745_01040" }, { "type": "cds", "sequence": "contig_1", "start": 588600, "stop": 589247, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTCGGGTCAGGGGACCCAAGTTAAGGGCAGTGTGCAGGGGAGTAGCCGAGCAGCTTCTGTTAGCGTTCAAACTACCCAGCAAAGCAGACAGCAATCGACTGATACCCAAGAATCGGAAGTGGTTAAGTTAGCCAAGTCCCTGCTGAAATCAAGTGCGGATTTGGCCAAACCCTTCACTGATAACCCCACCTTTAAAAAGGCCTTAACCGACATCCAATCCGAGTACAAGGATTATTTGGCTGCTGCGGAGAAGTTAAGTGAGTTTAAGAAAGATTTGGGGGAGGTGAGTGGCTTACAGCAAGCTATTATTGACCGCGCTGATAAGTACATTCAACTAGAAAAACAAGCCCAAAAGAGTGCGATTGGCTTGGGCCAACCGCTCCCTTACCAACGCGCTAGTGATGGTAGTTACCCCGCTTTAGAGAAGTTCTTTATTCCGGAAGATAGTGCAGCAGATGGAAAAGTAAAAGCATCTGAATCAGGATCTGCCGCTTTAGTAACACTAAAGACAACAGATAGTCAAAAAAGCACAAACACCGTTAAACAACCGGACATCAAACCGACTCGGGAAAACAACGACAAAAAGTTAAAGCAGTTAACTAGTGATGTAGAAACTAAAGCATCTAGTTTAATCACCAAATGA", "aa": "MGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKSLLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAEKLSEFKKDLGEVSGLQQAIIDRADKYIQLEKQAQKSAIGLGQPLPYQRASDGSYPALEKFFIPEDSAADGKVKASESGSAALVTLKTTDSQKSTNTVKQPDIKPTRENNDKKLKQLTSDVETKASSLITK", "aa_hexdigest": "7094b0dce8567c22b73531966c1282a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23165.593100000002, "isoelectric_point": 9.030682945251463 }, "id": "GIHJHMOALN_1045", "locus": "GCA009939745_01041" }, { "type": "cds", "sequence": "contig_1", "start": 589296, "stop": 590057, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_485", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75300", "UniRef:UniRef90_P75300" ], "nt": "GTGACCAAGTCCAACCTGCGCGGGGCCTTTAAGGGCGTGGGGTTAGCTACGGCCATTATTGACCAGTACGAGTACCTGGTGGGTGGGAAAAAAACGAGTTCGTTGCCCGAGGTCAAGGTAGATTCGAATAAGTCCAACCAAAATCCGCTGGATAGTTTTTTTATGGAGGGGAAGGATGCGGTGGCGATTAGATCCATAGTTTCGCGTGCGAAAATTGCCATGACCGACCAAACCCCCGGGTTTAAGGTCAACCCGGCGTTTGTCAAGGTGAAGCAGAGTCAGCAGAACGATACCTTTTACCAAAACCAGCGCAAACTGAGCGGCGGGCAAAGTGGGGATAATAACTCCCAGGGTAAACACCACTACCTCCAAGACGCGGTGCGGTTGACGAGTAGTCAAGCAATGGCGGCAGCTTCTACTGGGGCAGATTCTAGTAGTGGTACCAATGTTGGTGGTAGCTCCGGGGGCAACTCTGTTTTAATTCCGCTCCCAAGGAGTGCCGCCCTTACCCACACCCAGCAACAAGTTCAACAAACCACCTCCACACTCCAAACTCCTGTTTACGCCCGTGGTGACGATGGTACATACGCCCTGGCAATTGACGGTGGTGATTATTTTTTGGCGAACAACAAACGCGATTTCACCAAACAAGCCGACATCTTGCTGTACCGCTATTTACAAGCTAAGAGTAATAACTTTAAGGAAAACGGCGTCGAGTTTAGTTTGAACTTACTGGAATCTGGCAGCCTGTTCCAAACGTGA", "aa": "MTKSNLRGAFKGVGLATAIIDQYEYLVGGKKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVNPAFVKVKQSQQNDTFYQNQRKLSGGQSGDNNSQGKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQT", "aa_hexdigest": "0d8906a50234f53318bc3abe30f76133", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 588993, "stop": 590357, "observations": { "mutation": [ 589298, 590090 ], "stop": [ 589298, 590090 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75300", "query_cov": 1.0, "subject_cov": 0.49317738791423, "identity": 0.992, "score": 478.0, "evalue": 4.41e-166, "gene_start": 1, "gene_end": 253, "reference_start": 102, "reference_end": 354, "reference_sequence": "FFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFFMEGKDAVAIRSIVSRAKIAMTDQTPGFKVNPAFVKVKQSQQNDTFYQNQRKLSGGQSGDNNSQGKHHYLQDAVRLTSSQAMAAASTGADSSSGTNVGGSSGGNSVLIPLPRSAALTHTQQQVQQTTSTLQTPVYARGDDGTYALAIDGGDYFLANNKRDFTKQADILLYRYLQAKSNNFKENGVEFSLNLLESGSLFQTWAQTGLTAKLYGALVAMMGSGQGTQVKGSVQGSSRAASVSVQTTQQSRQQSTDTQESEVVKLAKSLLKSSADLAKPFTDNPTFKKALTDIQSEYKDYLAAAEKLSEFKKDLGEVSGLQQAIIDRADKYIQLEKQAQKSAIGLGQPLPYQRASDXVVTPL" }, "paralog": false, "identity": 0.9889867841409692, "score": 832.0, "evalue": 5.45e-308, "description": "Internal stop codon at 589298, 590090. Nonsense mutation around 589298, 590090.", "psc": { "uniref90_id": "UniRef90_P75300", "product": "Uncharacterized protein MPN_485", "uniref50_id": "UniRef50_P75300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75300", "UniRef:UniRef50_P75300" ] }, "pscc": { "uniref50_id": "UniRef50_P75300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75300" ], "product": "Uncharacterized protein MPN_485" } }, "id": "GIHJHMOALN_1046", "locus": "GCA009939745_01042" }, { "type": "cds", "sequence": "contig_1", "start": 590088, "stop": 590393, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE", "UniRef:UniRef90_UPI0006B4FEFE" ], "nt": "GTGGAGGAAAACCCCCTCCCCCTCACCCAAGCCTTCTTCGCGTACCAAGCACCCAAGGACGGGCTCGACAGCTTATACGACCAAGCCGCGATTGGTAGTGCCTTACAGTTGGGCTATGCCTTCCCCGCGTTTCGCGAGCCCAATAATGGACAAAGCCAAGGTAAAACCACCTTCGACCCCACCCCCAACAGTGCCCAAAACTTCGGGGATTTCATTAAGGCGGTGTTTCCGGAGCAGAAGAATGGTCAAACCCAACAATCCAATACATCCTCCCGCACCGGGTTGTTTGACTGGCAAACCAAGTGA", "aa": "MEENPLPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLGYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTK", "aa_hexdigest": "ed2067ba64981a82eb1574b3db4a6a8e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 589859, "stop": 590375, "observations": { "insertion": [ 589901 ], "mutation": [ 590090 ], "stop": [ 590090 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "query_cov": 0.9405940594059405, "subject_cov": 0.5397727272727273, "identity": 0.9890000000000001, "score": 191.0, "evalue": 1.07e-60, "gene_start": 7, "gene_end": 101, "reference_start": 6, "reference_end": 100, "reference_sequence": "MLYLFPLTQAFFAYQAPKDGLDSLYDQAAIGSALQLVYAFPAFREPNNGQSQGKTTFDPTPNSAQNFGDFIKAVFPEQKNGQTQQSNTSSRTGLFDWQTKWNSNGAANKLLVTKSNLRGAFKGVGLATAIIDQYEYLVGGSKTSSLPEVKVDSNKSNQNPLDSFLWRGRMPWRLDP" }, "paralog": false, "identity": 0.9766081871345029, "score": 333.0, "evalue": 9.19e-119, "description": "Internal stop codon at 590090. Frameshift due to insertion around 589901. Nonsense mutation around 590090.", "psc": { "uniref90_id": "UniRef90_UPI0006B4FEFE", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0006B4FEFE", "UniRef:UniRef50_UPI0006B4FEFE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0006B4FEFE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0006B4FEFE" ], "product": "DUF3713 domain-containing protein" } }, "id": "GIHJHMOALN_1047", "locus": "GCA009939745_01043" }, { "type": "cds", "sequence": "contig_1", "start": 590397, "stop": 590549, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGTTGACTGATGGTAGTGATCAGTTTAAACCCACTACCAAAGATAACCTCGACAGTACTAGCAACAAAATCAAGTTTGTTAATTCCAAACCAAATGATCCTAACGGGGAGTTCTTTGCTAACCTCCAGGCTTATCTATTTGCCCAGTGA", "aa": "MQLTDGSDQFKPTTKDNLDSTSNKIKFVNSKPNDPNGEFFANLQAYLFAQ", "aa_hexdigest": "55cc7661bf6274e0c4f2776d45f597bc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5627.124100000001, "isoelectric_point": 4.854868507385253 }, "id": "GIHJHMOALN_1048", "locus": "GCA009939745_01044" }, { "type": "cds", "sequence": "contig_1", "start": 590676, "stop": 590984, "strand": "-", "frame": 2, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE6F233", "UniRef:UniRef90_UPI002FE6F233" ], "nt": "GTGGTGCTAGCGGCGTGTTCTGCACTCAATACCTCCAATCTGTTTCCCCGTCAAAACCGCTCCAAGCAGTTGATTGGGTTTACCGAAAACAACATCATTAAACCTGAAGCTGTACTAAAAGCCGCTTTAGCTGAGGACAATGGTACGGAAACCATTTTAAGGGTTAACTTTGGTGAAGCGTTAAAGAGTTGGTACCAAAACAATAAGGATCGCAACATTGCTACCCGTTTAACTATCTTTAGTGAAAACGTGGAGGATGAACATGATAACCTGTTGGACCAAAAACAACAGGCCGAACCGATTAATTGA", "aa": "MVLAACSALNTSNLFPRQNRSKQLIGFTENNIIKPEAVLKAALAEDNGTETILRVNFGEALKSWYQNNKDRNIATRLTIFSENVEDEHDNLLDQKQQAEPIN", "aa_hexdigest": "807c034373d4ba9a80798388f61d708a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 590373, "stop": 590981, "observations": { "mutation": [ 590399, 590624, 590678 ], "stop": [ 590399, 590624, 590678 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE6F233", "query_cov": 0.9901960784313726, "subject_cov": 0.4469026548672566, "identity": 0.99, "score": 197.0, "evalue": 4.02e-62, "gene_start": 2, "gene_end": 102, "reference_start": 1, "reference_end": 101, "reference_sequence": "MLAACSALNTSNLFPRQNRSKQLIGFTENNIIKPEAVLKAALAEDNGTETILRVNFGEALKSWYQNNKDRNIATRLTIFSENVEDEHDNLLDQKQQAEPINWPIELQKEYDQWGGSESSWKALKLYDRLIADFQSLIFSNVVANVQLTDGSDQFKPTTKDNLDSTSNKIKFVNSKPNDPNGEFFANLQAYLFAQWVVEENPLPLTQAFFAYQAPKDGLDSLYDQAA" }, "paralog": false, "identity": 0.9752475247524752, "score": 390.0, "evalue": 8.39e-141, "description": "Internal stop codon at 590399, 590624, 590678. Nonsense mutation around 590399, 590624, 590678.", "psc": { "uniref90_id": "UniRef90_UPI002FE6F233", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI002FE6F233", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE6F233", "UniRef:UniRef50_UPI002FE6F233" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE6F233", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE6F233" ], "product": "DUF3713 domain-containing protein" } }, "id": "GIHJHMOALN_1049", "locus": "GCA009939745_01045" }, { "type": "cds", "sequence": "contig_1", "start": 591547, "stop": 592557, "strand": "-", "frame": 1, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "COG:COG0468", "COG:L", "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005829", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664", "KEGG:K03553", "RefSeq:WP_014325564.1", "SO:0001217", "UniParc:UPI00023869D3", "UniRef:UniRef100_A0AB33HMT0", "UniRef:UniRef50_P78014", "UniRef:UniRef90_P78014" ], "nt": "ATGGTACAAAAAGAAATGATTAATAAGAAAATTAGCCAAGACAACAGTTTTATTCAAAACAATAACTTGGCAGACTTTGATTTTTTAGATGCAAAGAAAAACAGTGAAATTAAAACTGTCTCAACTGGAAGCCTTCATTTAGATGAAGCGCTCGGCACTGGTGGTTTGCCACTAGGGCGCATTGTAGAACTTTATGGTAACGAGTCCTCGGGTAAAACCACGGTCGCGTTACATGCAGTCGCTAGCTTTCAAAAAGCGGGCAAAGTGGCCTGTTACATTGATGCTGAGGGTGCACTAGATTTAAGTTACGCCAAGGCAATTGGGATTGACTTGGGTAAATTACTAGTAGCCCATCCCAAGCATGGGGAAAACGCCTTTGCCCTAATGGAGTCACTGATTAAGACCAACAAAGTGGCCTTAATTGTGGTGGACTCCGTGGCGGCTTTGATTCCTAAGCAAGAACTGGAGGGCAACATGGATGACCAGACAATAGGACTCCATGCGCGGATGATGTCTAAGGGACTACGCAGTGTGCAGTCACTCTTACCTGAATCTGACACCTGCTTACTGTTTATTAACCAACTCCGGGAAAAACCCGGAGTCATGTTTGGTAATGGGGAAGTAACTACTGGGGGTCGGGCGTTGAAGTTCTACGCTTCGATGCGGATGGAGGCCAAACGCAGTGAACTGCTCAAAGACCGCTTTGGTAACTATGTCGGCATTAAGAGTAAGTTAACCGTGTCCAAGAATAAGGTAGCGCGCCCGTTTGGTGTTGCCTTTTTGGAAATTATGTTTAACCGCGGTATTGTGTATGAACACGAAGTGATTGAGTTAGCGCTTAAACACAATGTGGTGGTGCGCAGTGACAATGCATATTCGTTTAAGAGCCAAAACATTGCGATTGGCAAAGAGAAGCTCTTTAGCGTGTTGGCGGAAAAACCAGAGTTGTTTGAACAAATTAAGCAGCTCACGATTAAGCAAATCCACAGCCCGCCACCCCCAGCGAGCTAG", "aa": "MVQKEMINKKISQDNSFIQNNNLADFDFLDAKKNSEIKTVSTGSLHLDEALGTGGLPLGRIVELYGNESSGKTTVALHAVASFQKAGKVACYIDAEGALDLSYAKAIGIDLGKLLVAHPKHGENAFALMESLIKTNKVALIVVDSVAALIPKQELEGNMDDQTIGLHARMMSKGLRSVQSLLPESDTCLLFINQLREKPGVMFGNGEVTTGGRALKFYASMRMEAKRSELLKDRFGNYVGIKSKLTVSKNKVARPFGVAFLEIMFNRGIVYEHEVIELALKHNVVVRSDNAYSFKSQNIAIGKEKLFSVLAEKPELFEQIKQLTIKQIHSPPPPAS", "aa_hexdigest": "9d4a0c3fc0b38f1a3e74a8eb4cc8ec16", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00023869D3", "ncbi_nrp_id": "WP_014325564.1", "uniref100_id": "UniRef100_A0AB33HMT0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00023869D3", "RefSeq:WP_014325564.1", "UniRef:UniRef100_A0AB33HMT0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HMT0", "uniref90_id": "UniRef90_P78014" }, "psc": { "uniref90_id": "UniRef90_P78014", "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_P78014", "cog_id": "COG0468", "cog_category": "L", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005829", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664" ] }, "pscc": { "uniref50_id": "UniRef50_P78014", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78014" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "GIHJHMOALN_1050", "locus": "GCA009939745_01046" }, { "type": "cds", "sequence": "contig_1", "start": 592631, "stop": 593521, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_491", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75295", "UniRef:UniRef90_P75295" ], "nt": "ATGAAATTAACACTTTGGCTGGTGTTGGGCGCTGTCGGAGTCGGTGCAGTTGGTACGGGTGTGGGCTTTGGTACCAAGTACTTTTTAGACCACAAAGCCAAAAGTAACGTGGACGTTGCGTTTAAACAACGTTCACTACGCAAACAGGTCAATGTGGGCTTTTGGAATGCACTCAACTTCTTGGGTAGTGAAAGAAGTTTCGGCAACCAAAACAGTAAGGTTAAAACCAAGGGGATTGCTGAAATTATTAACCTCCTTAAGTACGACATTGTCGGTTTAGCGGAAATTCAACCATCCACTGAAAGTACTGCTGAACGGTTTGTCGACCAACTCAACGAGTACGCTGATCCTTATACCTCCTGGTCTTATGATCTATCTCCCGTAACTACCAGTAAGACTGCTAGTGAAGGCCAGAAGGAACGGATCTTAATTGTTTACAACAGCCAGAGTGTCAAAATGGAGGGACAAGGTTGGTTTTATGACAATCCCGAAATGGACATTAGTAACTATGTCAGTGATGGGAGTTCTTCCAAAACAAGAACACCTAAGAAAACTAAAACTAGTAAAAAGAAGCCTACTAAAAAGAAAAGTTCAAGAAGCAAATCTTCCAAAGGTAGTAAGAAACAAAAAACTACCAATGAAAGTGAAAGTGAAACTTTAGAACTCAAACTGGAACAACGCCGTGTAACAACACGCTCACAATCAAAGCAACAAAAGGGGCAAGAACAAGCAACTGATCAAACTGATAGTGAGGGTGTGACTACTGAAGAAGGAGCAGATAACACTGACACTGAATTAGTTGAAACCACAGCGGAAACAACCGAACAAGAAGCTACCACGGAAAGTACAAAAGACACTAAGGAAACTAAAGATAGAATCAAAGTGGACTGA", "aa": "MKLTLWLVLGAVGVGAVGTGVGFGTKYFLDHKAKSNVDVAFKQRSLRKQVNVGFWNALNFLGSERSFGNQNSKVKTKGIAEIINLLKYDIVGLAEIQPSTESTAERFVDQLNEYADPYTSWSYDLSPVTTSKTASEGQKERILIVYNSQSVKMEGQGWFYDNPEMDISNYVSDGSSSKTRTPKKTKTSKKKPTKKKSSRSKSSKGSKKQKTTNESESETLELKLEQRRVTTRSQSKQQKGQEQATDQTDSEGVTTEEGADNTDTELVETTAETTEQEATTESTKDTKETKDRIKVD", "aa_hexdigest": "ec14b66b91a21cd741a88872ee11b0df", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 592631, "stop": 593824, "observations": { "mutation": [ 593519 ], "stop": [ 593519 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75295", "query_cov": 1.0, "subject_cov": 0.6244725738396625, "identity": 0.9590000000000001, "score": 469.0, "evalue": 1.45e-162, "gene_start": 1, "gene_end": 296, "reference_start": 1, "reference_end": 296, "reference_sequence": "MKLTLWLVLGAVGVGAVGTGVGFGTKYFLDHKAKSNVDVAFKQRSLRKQVNVGFWNALNFLGSERSFGNQNSKVKTKGIAEIINLLKYDIVGLAEIQPSTESTAERFVDQLNEYADPYTSWSYDLSPVTTSKTASEGQKERILIVYNSQSVKMEGQGWFYDNPEMDISNYVSDGSSSKTRTPKKTKTSKKKPIKKKSSKSKSSKGSKKQKTTNESESETLELKLEQRRVTTRSQSKQQKGQEQATDQTDSEGVTTEEGADNTDTELVETTAETTEQEATTKSTKDTKETKDRTKVDWSRPPYSAEFSTQKGKVVVAFGHFDSPGANTKRGEKIAKMGKGQGAHEYFEAQTTFKAMDSIKQHFNNENILFMADTNIKFGNQAAAFGESKDYTFLTEDNEAWKSSLGTKSGYANPYDKIITSNSFKNKVLDQAETYWNYFDRYLKTKGHNSYLFDLASWGTKPRTISDHAPVGVLF" }, "paralog": false, "identity": 0.9697732997481109, "score": 694.0, "evalue": 1.08e-253, "description": "Internal stop codon at 593519. Nonsense mutation around 593519.", "psc": { "uniref90_id": "UniRef90_P75295", "product": "Uncharacterized protein MPN_491", "uniref50_id": "UniRef50_P75295", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75295", "UniRef:UniRef50_P75295", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P75295", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75295" ], "product": "Uncharacterized protein MPN_491" } }, "id": "GIHJHMOALN_1051", "locus": "GCA009939745_01047" }, { "type": "cds", "sequence": "contig_1", "start": 593573, "stop": 593830, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTTTGGTCATTTTGACTCTCCGGGGGCAAACACTAAGCGGGGTGAAAAAATCGCTAAGATGGGCAAGGGTCAAGGTGCCCATGAATACTTTGAAGCGCAAACTACCTTCAAGGCAATGGATTCCATTAAGCAGCACTTTAACAACGAAAACATCCTGTTTATGGCTGACACTAACATTAAATTCGGTAATCAAGCCGCTGCTTTTGGTGAAAGCAAAGATTACACCTTTTTAACTGAAGATAATGAAGCGTGA", "aa": "MAFGHFDSPGANTKRGEKIAKMGKGQGAHEYFEAQTTFKAMDSIKQHFNNENILFMADTNIKFGNQAAAFGESKDYTFLTEDNEA", "aa_hexdigest": "d8169903d19ba3a30488b160954a6716", "start_type": "GTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9459.345299999994, "isoelectric_point": 5.492601966857909 }, "id": "GIHJHMOALN_1052", "locus": "GCA009939745_01048" }, { "type": "cds", "sequence": "contig_1", "start": 594302, "stop": 594949, "strand": "-", "frame": 3, "gene": "ulaE", "product": "L-ribulose-5-phosphate 3-epimerase", "db_xrefs": [ "COG:COG3623", "COG:G", "EC:5.1.3.22", "GO:0005975", "GO:0016861", "GO:0019854", "GO:0034015", "SO:0001217", "UniRef:UniRef50_P75294", "UniRef:UniRef90_P75294" ], "nt": "TTGAGCATTGATGAATCGGATGCTAGGTTACAACGCTTGGATTGGAGTGATACCGAAATTAACCAACTCCACAATGAGTTACAAGCACAAACCTTTTGTTTAAACTCAATGTGTTTAAGTGCCCACCGGCGCTTTCCTTTGGGTTCCAAGAATAAAACTACTGTCCAACAGGGCTTAACCATTTTTGAAAAGGCCTGTGTGTTAGCACGCAAACTAGGGATTCGTATTATCCAGTTAGCGGCTTATGATGTGTATTATGAGCCACATGACACAGAAACAGAACGCAATTTCATTACTAACATGCGGAAAGTGGCAGAGTTAGCGCAAAAGTACGCTGTTACCATTGCCTTTGAGGTGATGGACACGCCGTTTGCTGGCACGATTGTGCGTTGTTTAAACTTGATTAAGCGGATTGGTAAGGCTAACATTTTGCTCTATCCGGATATTGGTAACCTATCGCAATTTAGTACGGCAGTCTTTGATGAAATCGCATTAGGTCAAGACAAGATAGTGGGCTTTCACTTTAAAGATACTTTACCCAAGCAGTTTAAGGAAGTACCGTTTGGCACAGGTACAGCCCAATTTGAAGCAGCTTTAAAAGCCATTCACCAGTATGTACCAACAGTACCAATTCTAATTGAAATGTGA", "aa": "MSIDESDARLQRLDWSDTEINQLHNELQAQTFCLNSMCLSAHRRFPLGSKNKTTVQQGLTIFEKACVLARKLGIRIIQLAAYDVYYEPHDTETERNFITNMRKVAELAQKYAVTIAFEVMDTPFAGTIVRCLNLIKRIGKANILLYPDIGNLSQFSTAVFDEIALGQDKIVGFHFKDTLPKQFKEVPFGTGTAQFEAALKAIHQYVPTVPILIEM", "aa_hexdigest": "5a012f6ffce7f5176551567a9fdeded2", "start_type": "TTG", "rbs_motif": null, "genes": [ "ulaE" ], "truncated": "both", "pseudogene": { "start": 594194, "stop": 595111, "observations": { "mutation": [ 594304, 595000 ], "stop": [ 594304, 595000 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75294", "query_cov": 1.0, "subject_cov": 0.7049180327868853, "identity": 0.995, "score": 427.0, "evalue": 4.19e-150, "gene_start": 1, "gene_end": 215, "reference_start": 55, "reference_end": 269, "reference_sequence": "MLVIHFKPYNNLKMSFTSTENKHLLGVYEKAINNKFAWKDKIAIAKQASFDFIELSIDESDARLQRLDWSDTEINQLHNELQAQTFCLNSMCLSAHRRFPLGSKNKTTVQQGLTIFEKACVLARKLGIRIIQLAAYDVYYEPHDTETERNFITNMRKVAELAQKYAVTIAFEVMDTPFAGTIVRCLNLIKRIGKANILLYPDIGNLSQFSTAVFDEIALGQDKIVGFHFKDTLPKQFKEVPFGTGTAQFEAALKAIHQYVPTVPILIEMWSKNDPAESTVQNVAQLKQAKQFYEQQWDLALKRVK" }, "paralog": false, "identity": 0.9934426229508196, "score": 599.0, "evalue": 3.54e-220, "description": "Internal stop codon at 594304, 595000. Nonsense mutation around 594304, 595000.", "psc": { "uniref90_id": "UniRef90_P75294", "gene": "ulaE", "product": "L-ribulose-5-phosphate 3-epimerase", "ec_ids": [ "5.1.3.22" ], "uniref50_id": "UniRef50_P75294", "cog_id": "COG3623", "cog_category": "G", "go_ids": [ "GO:0005975", "GO:0016861", "GO:0019854", "GO:0034015" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75294", "EC:5.1.3.22", "UniRef:UniRef50_P75294", "COG:COG3623", "COG:G", "GO:0005975", "GO:0016861", "GO:0019854", "GO:0034015" ] }, "pscc": { "uniref50_id": "UniRef50_P75294", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75294" ], "product": "Probable L-ribulose-5-phosphate 3-epimerase UlaE" } }, "id": "GIHJHMOALN_1053", "locus": "GCA009939745_01049" }, { "type": "cds", "sequence": "contig_1", "start": 595053, "stop": 595232, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACGCTTAGCGGATTTGGGCTTTAAGGTCACCGTTACCGGCGGCATTACCCTCAACGACATCCAGTTATTTAAGGATATTCCCATTTATATCTTTATTGCTGGGCGTACCATTAGGGATGCTAGTGATCCACTTCAAACCGTACAACAATTTAAAGATGAGTTTCACAAGTACTGA", "aa": "MKRLADLGFKVTVTGGITLNDIQLFKDIPIYIFIAGRTIRDASDPLQTVQQFKDEFHKY", "aa_hexdigest": "cf70789c30d3148d68fda7796cf1e1f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6787.792700000002, "isoelectric_point": 8.085318565368652 }, "id": "GIHJHMOALN_1054", "locus": "GCA009939745_01050" }, { "type": "cds", "sequence": "contig_1", "start": 595323, "stop": 595703, "strand": "-", "frame": 2, "gene": null, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "nt": "ATGGCTTTACCTTTAATCCAAATTGCGCTTGATAACCTCAGTTTAGCGAGTGCTTTAAATGACCTGGCTAAAGTCGGGGATGCTGTTGATGTTATTGAAGTGGGCACTATCTTACTAACAGCGGAGGGCGTTAATGCTGTTAAAGAAATTGCCAAACGCTATCCCCATAAGTTAATAGTGGCTGATGGCAAGATTGCTGATGCGGGGAAGGTGTTTAGCCAAATGTTCTTTGATGCTGGGGCACACTTTACCACGGTCATTTGTGCAGCGGAATTGCCCACCGTAAAGGATGTGGTAACTGTGGGTAACAGTTACACGCCCATAAAAGAAACCCAGGTGGAAATGACCAGTAACTTCACTTGGGAGCAGGTAACGCAGTGA", "aa": "MALPLIQIALDNLSLASALNDLAKVGDAVDVIEVGTILLTAEGVNAVKEIAKRYPHKLIVADGKIADAGKVFSQMFFDAGAHFTTVICAAELPTVKDVVTVGNSYTPIKETQVEMTSNFTWEQVTQ", "aa_hexdigest": "67d4e42584165d37aaba7faed86f9005", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387B8401", "query_cov": 0.8095238095238095, "subject_cov": 0.9902912621359223, "identity": 0.5, "score": 107.0, "evalue": 6.18e-28, "valid": false, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "uniref50_id": "UniRef50_UPI00387B8401" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B8401", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein" }, "genes": [], "id": "GIHJHMOALN_1055", "locus": "GCA009939745_01051" }, { "type": "cds", "sequence": "contig_1", "start": 595705, "stop": 595980, "strand": "-", "frame": 1, "gene": "ulaC", "product": "Ascorbate-specific PTS system EIIA component", "db_xrefs": [ "COG:COG1762", "COG:GT", "GO:0005737", "GO:0009401", "GO:0016301", "SO:0001217", "UniRef:UniRef50_P75292", "UniRef:UniRef90_P75292" ], "nt": "ATGCCTCATGCCCAGAATAACGGTACGGTACAGCAAAACTGCTTCTCTTTAGTGACCCTAAAGGAACCAGTTTACTTTGACAACGATCCCCGCCCGGTGCGCTTGTTAATTGGATTAGCGGCTACCAGCGCTGCCATTCATACGACAGAAGCTTTACCACAAATTGCGGCCCTAGTAGAAGATACTCAAGTGGTTAAGGACTTGTTGGACTGTTGTGATGAGACCAGCTTGTTTGCTGTGATTGATCGGGTTAACTTACACAAATATTTAACTTAA", "aa": "MPHAQNNGTVQQNCFSLVTLKEPVYFDNDPRPVRLLIGLAATSAAIHTTEALPQIAALVEDTQVVKDLLDCCDETSLFAVIDRVNLHKYLT", "aa_hexdigest": "2ccbafedad258642cb98db42bf2d82eb", "start_type": "ATG", "rbs_motif": null, "genes": [ "ulaC" ], "truncated": "3-prime", "pseudogene": { "start": 595705, "stop": 596184, "observations": { "mutation": [ 596112 ], "stop": [ 596112 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75292", "query_cov": 1.0, "subject_cov": 0.5723270440251572, "identity": 1.0, "score": 186.0, "evalue": 7.85e-59, "gene_start": 1, "gene_end": 91, "reference_start": 69, "reference_end": 159, "reference_sequence": "MTKLDFKAVLKQHHTVRLHQTAQDWKQAIQLCIHPLIAAKLVQPAYFDDILKSVAQYGPYFIIAENVAMPHAQNNGTVQQNCFSLVTLKEPVYFDNDPRPVRLLIGLAATSAAIHTTEALPQIAALVEDTQVVKDLLDCCDETSLFAVIDRVNLHKYLT" }, "paralog": false, "identity": 0.9937106918238994, "score": 316.0, "evalue": 1.22e-112, "description": "Internal stop codon at 596112. Nonsense mutation around 596112.", "psc": { "uniref90_id": "UniRef90_P75292", "gene": "ulaC", "product": "Ascorbate-specific PTS system EIIA component", "uniref50_id": "UniRef50_P75292", "cog_id": "COG1762", "cog_category": "GT", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75292", "UniRef:UniRef50_P75292", "COG:COG1762", "COG:GT", "GO:0005737", "GO:0016301", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75292", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75292" ], "product": "Ascorbate-specific PTS system EIIA component" } }, "id": "GIHJHMOALN_1056", "locus": "GCA009939745_01052" }, { "type": "cds", "sequence": "contig_1", "start": 596186, "stop": 596473, "strand": "-", "frame": 3, "gene": "ulaB", "product": "Ascorbate-specific PTS system EIIB component", "db_xrefs": [ "COG:COG3414", "COG:G", "EC:2.7.1.194", "GO:0005737", "GO:0008982", "GO:0009401", "GO:0016301", "RefSeq:WP_014325567.1", "SO:0001217", "UniParc:UPI0001DD6405", "UniRef:UniRef100_A0AAX0SP99", "UniRef:UniRef50_Q9EXD8", "UniRef:UniRef90_Q9EXD8" ], "nt": "ATGGAAAATAAGAAATTACACATTATTGCTGCTTGTGGTAACGGCATGGGTACGAGCATGCTAATCAAGATTAAGGTTGAAAAGATTATGAAGGAGTTGGGCTACACCGCCAAAGTGGAAGCTTTGTCAATGGGACAAACCAAGGGGATGGAGCACAGCGCTGACATTATTATTTCCTCGATTCACTTAACGAGTGAGTTTAACCCGAATGCTAAGGCCAAGATTGTTGGGGTGTTAAACCTGATGGATGAAAACGAAATTAAGCAAGCGTTAAGTAAGGTGCTTTAA", "aa": "MENKKLHIIAACGNGMGTSMLIKIKVEKIMKELGYTAKVEALSMGQTKGMEHSADIIISSIHLTSEFNPNAKAKIVGVLNLMDENEIKQALSKVL", "aa_hexdigest": "485936463dac84eda5cb31773b764513", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6405", "ncbi_nrp_id": "WP_014325567.1", "uniref100_id": "UniRef100_A0AAX0SP99", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6405", "RefSeq:WP_014325567.1", "UniRef:UniRef100_A0AAX0SP99" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SP99", "uniref90_id": "UniRef90_Q9EXD8" }, "psc": { "uniref90_id": "UniRef90_Q9EXD8", "gene": "ulaB", "product": "Ascorbate-specific PTS system EIIB component", "ec_ids": [ "2.7.1.194" ], "uniref50_id": "UniRef50_Q9EXD8", "cog_id": "COG3414", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD8" ], "product": "Ascorbate-specific PTS system EIIB component" }, "genes": [ "ulaB" ], "id": "GIHJHMOALN_1057", "locus": "GCA009939745_01053" }, { "type": "cds", "sequence": "contig_1", "start": 596476, "stop": 596592, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTACCGTTGGTTTAATCTTGCTGGCACAAATTATTGACAATGGTAAGCAAACTAAAGTAACTAAATTACAACAATTACTCAAAATTAACCCACAATTAGATTTAAATAACTAA", "aa": "MATVGLILLAQIIDNGKQTKVTKLQQLLKINPQLDLNN", "aa_hexdigest": "ea7e76d9ecf550aeada399359f77c771", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4201.970200000001, "isoelectric_point": 9.524575233459473 }, "id": "GIHJHMOALN_1058", "locus": "GCA009939745_01054" }, { "type": "cds", "sequence": "contig_1", "start": 596635, "stop": 597348, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGCCAGCTTAATTGCCATTATGACTGGGGTAAGGATGTTTATCACTGAGTTACAACAGGCGTTTCAAGGTATTAGTGAAAAGGTAGTACCAGGGGCCGTGGTAGCGGTTGATATTGCAGCTGTTTATGGGTTTTCAATTAACTCCGTGACCTTTGGGTTTTTAAGTGGGGTCATTGGTCAGTTTTTAGCTGTTGCCATTATGGCTGGCATTAGCTATATTCCGGGTAACCAGTTTAGCTTTGTGGCGATTCCTTTGTTTATTACACTCTTCTTTAACAGTGGTGCCTTTGGCGTGTATGCTAATGCCGAGGGTGGTTGGAAGGCGGCCTTACTGCTCCCGGGTATTATTGGCTTTTTAGAAATTATTGTGATTTCATTTGCGCTGCGCACCGTATCAAACGCTTACCAGGCTTCGGCACTGTTAGATGCTAAACAAAGCTTTGATTTAAAGGCGTTAATGGGTAATAGTTTGGACAATAACAACGGTTCGGCATTAAAAACAGCCGTAGAAAAGGTTGTAAATGCTACTGGTGTTAACCGGATAACCGAAGCGCAAAGCTTTGTTAAATCCGATAGCTTTAACAGTTTAAAAGCTGTTAACAGTACGCTAGCACAAGCAATTGAGTGGATCAGTAAAAGTGCATCACCCGTTGACAACGGTTTTATTGGAATGGCTGACTGGAATCTTTACTTTGGGTTGTTAGTTTGA", "aa": "MVASLIAIMTGVRMFITELQQAFQGISEKVVPGAVVAVDIAAVYGFSINSVTFGFLSGVIGQFLAVAIMAGISYIPGNQFSFVAIPLFITLFFNSGAFGVYANAEGGWKAALLLPGIIGFLEIIVISFALRTVSNAYQASALLDAKQSFDLKALMGNSLDNNNGSALKTAVEKVVNATGVNRITEAQSFVKSDSFNSLKAVNSTLAQAIEWISKSASPVDNGFIGMADWNLYFGLLV", "aa_hexdigest": "fa2c958b629bea6f4d56c8ada9842548", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25030.702300000004, "isoelectric_point": 5.06540012359619 }, "id": "GIHJHMOALN_1059", "locus": "GCA009939745_01055" }, { "type": "cds", "sequence": "contig_1", "start": 597406, "stop": 597885, "strand": "-", "frame": 1, "gene": null, "product": "PTS ascorbate transporter subunit IIC", "db_xrefs": [ "KEGG:K03475", "SO:0001217", "UniRef:UniRef50_UPI002244F519", "UniRef:UniRef90_UPI002244F519" ], "nt": "ATGGTTACTGGACACGTTATGTTGCAACAAGCAGCGGTAGTTACTACGCTGTTCTATATTGTCTTGTTCCGCCAAATTCCGTTGTTGGGTACGGGCATTGCTTACGGTGCCCAAGCCGGTCTAGTGATTATTAGTGGTATCTTTTTGGGAGTTTACTGGTCTACGGCCTCTACGGGTACCTATTTAGTGACCAATAAGGTAACCAATAATGCTGGTTTTTCGATTGGTCACCAGCAGATGTTAGGGATTATGACGGTTGCTAAATTGGGCAAGTACTTTGGTGACAAAAACGATAGTGCCGAGCACAAAAAACTGCCTAAGGCCCTAAAGATCTTTGAAGATAATATCTTCACCCAAACGATCATTATCCTTAGTTTGTTTGTGGTGTTGTTTATTGTGATTTTGGCTAGTTACAAGGGTGATAAACCACTGTTAATTAACTGGGACAAATTTGGTGCCATTAATGGTTTAGAGATTTGA", "aa": "MVTGHVMLQQAAVVTTLFYIVLFRQIPLLGTGIAYGAQAGLVIISGIFLGVYWSTASTGTYLVTNKVTNNAGFSIGHQQMLGIMTVAKLGKYFGDKNDSAEHKKLPKALKIFEDNIFTQTIIILSLFVVLFIVILASYKGDKPLLINWDKFGAINGLEI", "aa_hexdigest": "c5228fc0191eff5d5115782c24e927bd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 597450, "stop": 598185, "observations": { "insertion": [ 597459 ], "mutation": [ 597906 ], "stop": [ 597906 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002244F519", "query_cov": 0.8930817610062893, "subject_cov": 0.42388059701492536, "identity": 1.0, "score": 270.0, "evalue": 1.29e-88, "gene_start": 1, "gene_end": 142, "reference_start": 192, "reference_end": 333, "reference_sequence": "MLNKIKLQQKRKLIIGWSVFALINLVVILLTVLLRAGLPNLVSKGVTPFSAQAFGDAFIFLITRVYLDNFLRQPALLLGVITLIGYLALGRGGVQSVVGALKTVIGFILLSIGSGVLVSTARPVFDTIKGLGGTGVVLLDPYFSLASANDFFTNSFLNNDYVSLIAFSLLVGFIVNIIFVGLKRWTNTNSIMVTGHVMLQQAAVVTTLFYIVLFRQIPLLGTGIAYGAQAGLVIISGIFLGVYWSTASTGTYLVTNKVTNNAGFSIGHQQMLGIMTVAKLGKYFGDKNDSAEHKKLPKALKIFEDNIFTQTIIILSLFVVLFIVILASYKGDKHC" }, "paralog": false, "identity": 0.9959016393442623, "score": 464.0, "evalue": 3.21e-167, "description": "Internal stop codon at 597906. Frameshift due to insertion around 597459. Nonsense mutation around 597906.", "psc": { "uniref90_id": "UniRef90_UPI002244F519", "product": "PTS ascorbate transporter subunit IIC", "uniref50_id": "UniRef50_UPI002244F519", "kegg_orthology_id": "K03475", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002244F519", "UniRef:UniRef50_UPI002244F519", "KEGG:K03475" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002244F519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002244F519" ], "product": "PTS ascorbate transporter subunit IIC" } }, "id": "GIHJHMOALN_1060", "locus": "GCA009939745_01056" }, { "type": "cds", "sequence": "contig_1", "start": 597904, "stop": 598383, "strand": "-", "frame": 1, "gene": null, "product": "PTS ascorbate transporter subunit IIC", "db_xrefs": [ "KEGG:K03475", "SO:0001217", "UniRef:UniRef50_UPI002244F519", "UniRef:UniRef90_UPI002244F519" ], "nt": "GTGGTAATTCTCTTGACAGTATTACTGCGTGCAGGATTACCGAACTTAGTTAGTAAGGGAGTAACCCCGTTTTCGGCCCAAGCCTTTGGTGATGCCTTTATCTTTTTAATTACGCGGGTATACCTCGATAACTTTTTGCGTCAACCAGCGCTATTGTTAGGTGTTATTACCCTAATTGGTTACCTTGCTTTAGGTCGTGGTGGGGTGCAATCGGTGGTGGGGGCACTAAAGACCGTAATTGGCTTTATCCTGTTAAGCATTGGTAGTGGCGTGTTGGTGTCCACCGCCCGTCCCGTTTTTGACACGATTAAGGGTTTAGGGGGTACTGGTGTGGTACTATTGGATCCTTACTTTTCCTTAGCATCAGCGAACGATTTCTTTACGAACAGTTTCTTGAACAATGACTATGTGTCCTTGATTGCCTTTTCGCTGTTAGTGGGCTTTATTGTCAACATTATCTTTGTGGGGTTAAAGCGTTGA", "aa": "MVILLTVLLRAGLPNLVSKGVTPFSAQAFGDAFIFLITRVYLDNFLRQPALLLGVITLIGYLALGRGGVQSVVGALKTVIGFILLSIGSGVLVSTARPVFDTIKGLGGTGVVLLDPYFSLASANDFFTNSFLNNDYVSLIAFSLLVGFIVNIIFVGLKR", "aa_hexdigest": "727f362a7aa2c1218afdd2e34c123151", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 597601, "stop": 598458, "observations": { "mutation": [ 597906, 598410 ], "stop": [ 597906, 598410 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002244F519", "query_cov": 0.9937106918238994, "subject_cov": 0.4716417910447761, "identity": 1.0, "score": 288.0, "evalue": 9.59e-96, "gene_start": 2, "gene_end": 159, "reference_start": 27, "reference_end": 184, "reference_sequence": "MLNKIKLQQKRKLIIGWSVFALINLVVILLTVLLRAGLPNLVSKGVTPFSAQAFGDAFIFLITRVYLDNFLRQPALLLGVITLIGYLALGRGGVQSVVGALKTVIGFILLSIGSGVLVSTARPVFDTIKGLGGTGVVLLDPYFSLASANDFFTNSFLNNDYVSLIAFSLLVGFIVNIIFVGLKRWTNTNSIMVTGHVMLQQAAVVTTLFYIVLFRQIPLLGTGIAYGAQAGLVIISGIFLGVYWSTASTGTYLVTNKVTNNAGFSIGHQQMLGIMTVAKLGKYFGDKNDSAEHKKLPKALKIFEDNIFTQTIIILSLFVVLFIVILASYKGDKHC" }, "paralog": false, "identity": 0.9929824561403509, "score": 537.0, "evalue": 3.96e-196, "description": "Internal stop codon at 597906, 598410. Nonsense mutation around 597906, 598410.", "psc": { "uniref90_id": "UniRef90_UPI002244F519", "product": "PTS ascorbate transporter subunit IIC", "uniref50_id": "UniRef50_UPI002244F519", "kegg_orthology_id": "K03475", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002244F519", "UniRef:UniRef50_UPI002244F519", "KEGG:K03475" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002244F519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002244F519" ], "product": "PTS ascorbate transporter subunit IIC" } }, "id": "GIHJHMOALN_1061", "locus": "GCA009939745_01057" }, { "type": "cds", "sequence": "contig_1", "start": 598458, "stop": 598601, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F32274", "UniRef:UniRef100_A0AB38W6G9", "UniRef:UniRef50_A0AB38W6G9", "UniRef:UniRef90_A0AB38W6G9" ], "nt": "GTGGGAGTTTCCGATGCGGCCATTAACACCATCTTGGTCGAAAATCCCGCTGAAATCCTGGCCTTTGATGCACCGCGGAAGTTTAATCCCAAGGCTGTGCATCCACGGGTATTAGCACTTAAAAAACAACTAAAAATAGAGTAA", "aa": "MGVSDAAINTILVENPAEILAFDAPRKFNPKAVHPRVLALKKQLKIE", "aa_hexdigest": "d2481e296c7fa3b06f9740f390960dd9", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002F32274", "uniref100_id": "UniRef100_A0AB38W6G9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F32274", "UniRef:UniRef100_A0AB38W6G9" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6G9", "uniref90_id": "UniRef90_A0AB38W6G9" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6G9", "uniref50_id": "UniRef50_A0AB38W6G9" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6G9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5168.0659, "isoelectric_point": 9.397636985778806 }, "id": "GIHJHMOALN_1062", "locus": "GCA009939745_01058" }, { "type": "cds", "sequence": "contig_1", "start": 598667, "stop": 598822, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002FEEE66", "UniRef:UniRef100_A0AB38W6Q4", "UniRef:UniRef50_A0AB38W6Q4", "UniRef:UniRef90_A0AB38W6Q4" ], "nt": "GTGACCCTGTGTTTTGATGGGCCCGATCGGGTGAAGTATTACCCCGATTGTTTATTGGCTAAACACATTAAATACTTAGTGGACTTGGGCTTTGTTAAACACATTACCCTGGCCTTAGATGCGGGGCGGGTACTGTACCAAAACACTATGGTTTAG", "aa": "MTLCFDGPDRVKYYPDCLLAKHIKYLVDLGFVKHITLALDAGRVLYQNTMV", "aa_hexdigest": "4c2ee55de2c9cbfbff0f2f645c7c6ee5", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002FEEE66", "uniref100_id": "UniRef100_A0AB38W6Q4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002FEEE66", "UniRef:UniRef100_A0AB38W6Q4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6Q4", "uniref90_id": "UniRef90_A0AB38W6Q4" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6Q4", "uniref50_id": "UniRef50_A0AB38W6Q4" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6Q4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5859.9022, "isoelectric_point": 7.79804744720459 }, "id": "GIHJHMOALN_1063", "locus": "GCA009939745_01059" }, { "type": "cds", "sequence": "contig_1", "start": 598886, "stop": 599317, "strand": "-", "frame": 3, "gene": null, "product": "Phosphotriesterase-related protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00023869D4", "UniRef:UniRef100_A0AAV5N8W9", "UniRef:UniRef50_A0AAV5N8W9", "UniRef:UniRef90_A0AAV5N8W9" ], "nt": "ATGGATCCACCGAATGTGGGCCGTGATGTCAAACGGATGGTAGCGATTGCAGAACAACTAAAGGGCAAGCTGAACATTATTATGGCCACTGGTTTTCACAAGGCTGCTTTCTATGACAAGGGAAGTTCTTGGTTAGCACAAGTGCCAGTTAATGAAATTGTGCCGATGTTGGTCGCTGAAATAGAAGAGGGTATGGATTTGTATAACTATTCCGGTCCCGTGGTTAAACGGGGCAAGGCTAAGGCAGGCATTATTAAAGCTGGTACGGGCTATGCTGCGATTGACCGGTTGGAATTAAAGGCCTTGGAAGCAGTCGCGATTACTTCGATTACTACTGGTGCTCCCGTACTCGTGCACACCCAACTCGGTACCATGGCATATGAAGCAGTACAACACCTGATTGATTTTGGCGTTAATCCGCGGAAAATTTAA", "aa": "MDPPNVGRDVKRMVAIAEQLKGKLNIIMATGFHKAAFYDKGSSWLAQVPVNEIVPMLVAEIEEGMDLYNYSGPVVKRGKAKAGIIKAGTGYAAIDRLELKALEAVAITSITTGAPVLVHTQLGTMAYEAVQHLIDFGVNPRKI", "aa_hexdigest": "505079639ef0fffb8c756657b2841ab0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00023869D4", "uniref100_id": "UniRef100_A0AAV5N8W9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00023869D4", "UniRef:UniRef100_A0AAV5N8W9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N8W9", "uniref90_id": "UniRef90_A0AAV5N8W9" }, "psc": { "uniref90_id": "UniRef90_A0AAV5N8W9", "product": "Phosphotriesterase-related protein", "uniref50_id": "UniRef50_A0AAV5N8W9" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N8W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N8W9" ], "product": "Phosphotriesterase-related protein" }, "genes": [], "id": "GIHJHMOALN_1064", "locus": "GCA009939745_01060" }, { "type": "cds", "sequence": "contig_1", "start": 599351, "stop": 599512, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_497", "db_xrefs": [ "GO:0008270", "GO:0009056", "GO:0016787", "SO:0001217", "UniParc:UPI0001DD6406", "UniRef:UniRef100_P75290", "UniRef:UniRef50_Q4A724", "UniRef:UniRef90_P75290" ], "nt": "ATGAAAAGATTTGTAAGAACAGTTTTAGGGGACATTGACCCTAAGGATTTAGGTATTTGTGACTGTCACGATCATTTAATTAAGAATTGGGGTCCGGAAGCCAAAGAGCACCCTGATTTTGTGATGTTATCGAATGAAGCTGCTATTAAAGAGTGTTTGTAA", "aa": "MKRFVRTVLGDIDPKDLGICDCHDHLIKNWGPEAKEHPDFVMLSNEAAIKECL", "aa_hexdigest": "72db58d0e68b6a7a87116caca0a28e08", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6406", "uniref100_id": "UniRef100_P75290", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6406", "UniRef:UniRef100_P75290" ] }, "ips": { "uniref100_id": "UniRef100_P75290", "uniref90_id": "UniRef90_P75290" }, "psc": { "uniref90_id": "UniRef90_P75290", "product": "Uncharacterized protein MPN_497", "uniref50_id": "UniRef50_Q4A724", "go_ids": [ "GO:0016787", "GO:0008270", "GO:0009056" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A724", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A724" ], "product": "Phospho-furanose lactonase" }, "genes": [], "id": "GIHJHMOALN_1065", "locus": "GCA009939745_01061" }, { "type": "cds", "sequence": "contig_1", "start": 599628, "stop": 600356, "strand": "+", "frame": 3, "gene": "ulaF", "product": "putative L-ribulose-5-phosphate 4-epimerase UlaF", "db_xrefs": [ "COG:COG0235", "COG:EG", "EC:5.1.3.4", "GO:0005829", "GO:0008270", "GO:0008742", "GO:0016832", "GO:0019323", "GO:0019852", "GO:0019854", "RefSeq:WP_014325569.1", "SO:0001217", "UniParc:UPI0001DD6407", "UniRef:UniRef100_A0AAX0S215", "UniRef:UniRef50_P75289", "UniRef:UniRef90_P75289" ], "nt": "ATGGATCAAAAAATGATAAATGACCTCAAAGAACAGGTTTTTCAAACGAATTTATTACTACCCAAATATGGCTTAGTCATCCACACTTGGGGTAATGTTTCGATGATTGCCCCGAACCGTCAGTTCTTTGTCATTAAACCAAGTGGTGTGAGCTATGACAAGATGCGTGCCCAAGATATGGTAGTGGTCGATTTGGACAACAATGTTCTAGATACCAACGGCCTCAAACCATCAAGTGATACCCCAACCCATGCGTTAATGTACAAACATTGTCCAGATATTAAAGCTATTGTGCACACCCACTCTACTTTAGCGACATCGTTTGCTCAAGCCGATAAGCCGATTCCTTGTCTTGGTACTACCCATGCTGATAACTTCTTTGGACCAATCCCGTGTACTAGGGCTTTAAGTGACAGTGAAATTAATGGAGCTTATGAACATAATACTGGTTTAGTCATTCTGGAACACCTCAAAAACAACCAGGTTGATGTCAATGCTTGTGCGGCCATCTTAGTCAAAGAACACGGTTCGTTTGTTTGGTCCAATAAGAATGGCAAGGATGCAGTGGACCGGGCGTTAACTTTAGAACAAGTGGCCCAAATGGCCTTATATACCCAAATGATCAATCCCCACATGAAAGAAGCAAACCCAGCGTTACAACAAAAGCACTATAACCGCAAACATGGTAAAGATGCTTACTATGGACAAAATACTAAGCAAGAAGATTAA", "aa": "MDQKMINDLKEQVFQTNLLLPKYGLVIHTWGNVSMIAPNRQFFVIKPSGVSYDKMRAQDMVVVDLDNNVLDTNGLKPSSDTPTHALMYKHCPDIKAIVHTHSTLATSFAQADKPIPCLGTTHADNFFGPIPCTRALSDSEINGAYEHNTGLVILEHLKNNQVDVNACAAILVKEHGSFVWSNKNGKDAVDRALTLEQVAQMALYTQMINPHMKEANPALQQKHYNRKHGKDAYYGQNTKQED", "aa_hexdigest": "e1ac05b81cef137a8c8257c0649af3bd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6407", "ncbi_nrp_id": "WP_014325569.1", "uniref100_id": "UniRef100_A0AAX0S215", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6407", "RefSeq:WP_014325569.1", "UniRef:UniRef100_A0AAX0S215" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S215", "uniref90_id": "UniRef90_P75289" }, "psc": { "uniref90_id": "UniRef90_P75289", "gene": "ulaF", "product": "Probable L-ribulose-5-phosphate 4-epimerase UlaF", "ec_ids": [ "5.1.3.4" ], "uniref50_id": "UniRef50_P75289", "cog_id": "COG0235", "cog_category": "EG", "go_ids": [ "GO:0005829", "GO:0016832", "GO:0008742", "GO:0008270", "GO:0019854", "GO:0019852", "GO:0019323" ] }, "pscc": { "uniref50_id": "UniRef50_P75289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75289" ], "product": "Probable L-ribulose-5-phosphate 4-epimerase UlaF" }, "genes": [ "ulaF" ], "id": "GIHJHMOALN_1066", "locus": "GCA009939745_01062" }, { "type": "cds", "sequence": "contig_1", "start": 600310, "stop": 600801, "strand": "+", "frame": 1, "gene": null, "product": "MPN499 family protein", "db_xrefs": [ "RefSeq:WP_010874855.1", "SO:0001217", "UniParc:UPI000013AB8A", "UniRef:UniRef100_P75288", "UniRef:UniRef50_P75288", "UniRef:UniRef90_P75288" ], "nt": "ATGGTAAAGATGCTTACTATGGACAAAATACTAAGCAAGAAGATTAAAGTGAACTGGTTGGGCGGGGTGTTTTGGTTATTACCTAACTTACTCGATCTGTTTTCCGCTAGCAAACGTAAAGCGAGTGTGCGACCCTACCAAAGCTTATTGGAACTGGTTCAAGAAAACTTCTTGAACCGGTATGACCTCGTGCACTTTAGCTTTAATGGTGACCATGACTTCTTTCACTTTAATGATTTACAAGCCATTAGGAGCTTTAACTTTACGATAGAAGAGGAACAACTAGGAGCAATGCAACCAGATGAGGTTTTACTGTTTGAACCTGTGGATCGTGTAACAGTGGAATTAGATCAAAAGGGTTTGAGTTTAATCCACAGTGGCAAAGCTTTTTGTGCTAGTGCAAACTACTTTAAGCACTGGCTAAAACGCGTACCACAGCAAGACAAAGTGACCCTAGTGTGGCGAAAATCAGGTTTTGAATTAAAGCAGTAG", "aa": "MVKMLTMDKILSKKIKVNWLGGVFWLLPNLLDLFSASKRKASVRPYQSLLELVQENFLNRYDLVHFSFNGDHDFFHFNDLQAIRSFNFTIEEEQLGAMQPDEVLLFEPVDRVTVELDQKGLSLIHSGKAFCASANYFKHWLKRVPQQDKVTLVWRKSGFELKQ", "aa_hexdigest": "9c705784d6f0e311001497b1cc97e276", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AB8A", "ncbi_nrp_id": "WP_010874855.1", "uniref100_id": "UniRef100_P75288", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AB8A", "RefSeq:WP_010874855.1", "UniRef:UniRef100_P75288" ] }, "ips": { "uniref100_id": "UniRef100_P75288", "uniref90_id": "UniRef90_P75288" }, "psc": { "uniref90_id": "UniRef90_P75288", "product": "MPN499 family protein", "uniref50_id": "UniRef50_P75288" }, "pscc": { "uniref50_id": "UniRef50_P75288", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75288" ], "product": "Uncharacterized protein MPN_499" }, "genes": [], "id": "GIHJHMOALN_1067", "locus": "GCA009939745_01063" }, { "type": "cds", "sequence": "contig_1", "start": 600790, "stop": 601146, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCGAGGGTGGGTCATTTGGGAATATTGTTCCCCGTAGTCGTCGTCTGACTGCCCCCGAGGGTGCCCTGGACATTCGCTTTGAGGCCAACGCCGACATTCGAAAACGTCGGGAGAAAGGAACGCGAACCACTTGTGTTCGTACTAGTTCCGCTGGACGACCCCGCGCTGGTTTGAGGCGCGGTTATATCATCCACACCCCGGTGGTTATTCCCATACCTGTACCCATTCCCAATCCAGGGAATATAGTCCAACACCTGGTCGAGCACCGACCCCGCGAGGTTGAACCCGGTGGAGAAACGGGAAAGCGTGGGGGAGAAGTGCTATATAGTATTAGAGTAATTCTACTGCTTTAA", "aa": "MVEGGSFGNIVPRSRRLTAPEGALDIRFEANADIRKRREKGTRTTCVRTSSAGRPRAGLRRGYIIHTPVVIPIPVPIPNPGNIVQHLVEHRPREVEPGGETGKRGGEVLYSIRVILLL", "aa_hexdigest": "3493e9d32e5569d42c9a76f124f0b156", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12977.882299999988, "isoelectric_point": 11.19572467803955 }, "id": "GIHJHMOALN_1068", "locus": "GCA009939745_01064" }, { "type": "cds", "sequence": "contig_1", "start": 601513, "stop": 601803, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTTAATAAGTCCGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGAGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAGTGGCACAAACACAACGTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGA", "aa": "MLVNKSGQDDNSKFKAEDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRASGSGSNTTSSPGIGFKIPEQSGTNTTSKAVLITPGLA", "aa_hexdigest": "890f22afdbdbb58513e9f1ad165ff405", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10171.074699999988, "isoelectric_point": 5.170722770690916 }, "id": "GIHJHMOALN_1069", "locus": "GCA009939745_01065" }, { "type": "cds", "sequence": "contig_1", "start": 602026, "stop": 602529, "strand": "+", "frame": 1, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593", "UniRef:UniRef90_UPI001330D5D0" ], "nt": "GTGGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTATACCGACAAGGATTTGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCCCTACCTCCATTTTATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGAGGAGTAGTGGTAACCTCAGTAGTGTGTTTAGTGGTAGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAA", "aa": "MESVWDFKGVWADQAQLAAQAATSSTTTTATGATLPEHPNALAYQISYTDKDLYKASTQGSGQTNSQNNSPYLHFIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGRSSGNLSSVFSGRGAGGGSSGSGQSGVDLSPVERVSGH", "aa_hexdigest": "f024ade581ead5db44fe8f6d892c32ab", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 601726, "stop": 602529, "observations": { "mutation": [ 601801, 601984 ], "stop": [ 601801, 601984 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001330D5D0", "query_cov": 1.0, "subject_cov": 0.46005509641873277, "identity": 0.988, "score": 306.0, "evalue": 4.83e-102, "gene_start": 1, "gene_end": 167, "reference_start": 197, "reference_end": 363, "reference_sequence": "ALSFTNKNNAQRAQLFLRGLLGSIPVLVNKSGQDDNSKFKAEDQKWSYTDLQSDQTKLNLPAYGEVNGLLNPALVETYFGNTRASGSGSNTTSSPGIGFKIPEQSGTNTTSKAVLITPGLAWTPQDVGNLVVSGTSFSFQLGGWLVTFTDFIKPRAGYLGLQLTGLDVSEATQRELIWAKRPWAAFRGSWVNRLGRVESVWDFKGVWADQAQLAAQAATSSTTTTATGATLPEHPNALAYQISYTDKDLYKASTQGSGQTNSQNNSPYLHFIKPKKVESTTQLDQGLKNLLDPNQVRTKLRQSFGTDHSTQPQPQSLKTTTPVFGTSSGNLSSVFSGRGAGGGSSGSGQSGVDLSPVERVSGH" }, "paralog": false, "identity": 0.9887640449438202, "score": 513.0, "evalue": 5.77e-186, "description": "Internal stop codon at 601801, 601984. Nonsense mutation around 601801, 601984.", "psc": { "uniref90_id": "UniRef90_UPI001330D5D0", "product": "adhesin", "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001330D5D0", "UniRef:UniRef50_P75593" ] }, "pscc": { "uniref50_id": "UniRef50_P75593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75593" ], "product": "Putative adhesin P1-like protein MPN_099" } }, "id": "GIHJHMOALN_1070", "locus": "GCA009939745_01066" }, { "type": "cds", "sequence": "contig_1", "start": 602596, "stop": 602895, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640A", "UniRef:UniRef100_A0A0H3DNZ5", "UniRef:UniRef50_A0A0H3DNZ5", "UniRef:UniRef90_A0A0H3DNZ5" ], "nt": "GTGTCTTTACCAACTTTAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGGCTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTGTTGGCGCTTCTCAATAAACTGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAA", "aa": "MSLPTLSNLVNEYPKSMKLVKLARLHRFLKQDQLQLVPLFSPNFLQVLLALLNKLVNLFSENTTMKTNKKYAFAALLMQAQAKLPNSFKFFLSKKGGMA", "aa_hexdigest": "9cdb1adeccaa0056dddaece30460748d", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD640A", "uniref100_id": "UniRef100_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640A", "UniRef:UniRef100_A0A0H3DNZ5" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DNZ5", "uniref90_id": "UniRef90_A0A0H3DNZ5" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DNZ5", "uniref50_id": "UniRef50_A0A0H3DNZ5" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DNZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DNZ5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11274.444899999984, "isoelectric_point": 10.390707969665527 }, "id": "GIHJHMOALN_1071", "locus": "GCA009939745_01067" }, { "type": "cds", "sequence": "contig_1", "start": 603063, "stop": 603485, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAACACGGAATACGTAACGCGTAAAGAGTTTAACGAGTACAAAAACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGCGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGCTTGGACAAGATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAA", "aa": "MNTEYVTRKEFNEYKNSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQIEQKAQGETLKLILQTLQKMSDRLDKMEVKMDKMEVKMDKMEVKMDKMEKRIDKLESK", "aa_hexdigest": "98cf4b48d6fb587165a7a107173fd118", "start_type": "GTG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 54, "aa_cov": 0.38571428571428573, "hmm_cov": 1.0, "evalue": 4.7005369214085677e-39, "score": 128.6022491455078, "start": 2, "stop": 55 } ], "seq_stats": { "molecular_weight": 16485.8895, "isoelectric_point": 6.6398401260375985 }, "id": "GIHJHMOALN_1072", "locus": "GCA009939745_01068" }, { "type": "cds", "sequence": "contig_1", "start": 603929, "stop": 604186, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGCGCGATAACATTGCCAAGGGCATCACTGCCGGTAGTAACACCCAACAAACCACGTATGATCCCACCCGAACCGAGGCCACCTTGACCACCGCCACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGGCTGAGTGGGGCTGCACCCCAACAGTGA", "aa": "MRDNIAKGITAGSNTQQTTYDPTRTEATLTTATTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQ", "aa_hexdigest": "f069261336431c534981970471d38417", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 58, "aa_cov": 0.6823529411764706, "hmm_cov": 0.896551724137931, "evalue": 5.152436318228413e-09, "score": 31.335514068603516, "start": 27, "stop": 84 } ], "seq_stats": { "molecular_weight": 9232.065799999997, "isoelectric_point": 8.137860298156738 }, "id": "GIHJHMOALN_1073", "locus": "GCA009939745_01069" }, { "type": "cds", "sequence": "contig_1", "start": 604217, "stop": 604726, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_502", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571", "UniRef:UniRef90_P75285" ], "nt": "GTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAAAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCTATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCCAACGTGGGGGTTCGTCAAATGAAAACAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGA", "aa": "MAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGYESKSSNGSSSTSTTQRGGSSNENKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTP", "aa_hexdigest": "780379d1e8039b126587944a38c67f1a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 603929, "stop": 605029, "observations": { "mutation": [ 604184, 604724, 604886, 604901, 604925 ], "stop": [ 604184, 604724, 604886, 604901, 604925 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75285", "query_cov": 1.0, "subject_cov": 0.4004739336492891, "identity": 0.9940000000000001, "score": 255.0, "evalue": 2.89e-81, "gene_start": 1, "gene_end": 169, "reference_start": 97, "reference_end": 265, "reference_sequence": "MRDNIAKGITAGSNTQQTTYDPTRTEATLTTATTFALRRYDLAGRALYDLDFSKLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKDKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGYESKSSNGSSSTSTTQRGGSSNENKVKALQVAVKKKSGSQGNSGDQGTEQVELESNDLANAPIKRGSNNNQQVQLKADDFGTAPSSSGSGTQDGTPTPWTPWLTTEQIHNDPAKFAASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFEQALLVFGICLS" }, "paralog": false, "identity": 0.9836065573770492, "score": 662.0, "evalue": 4.67e-244, "description": "Internal stop codon at 604184, 604724, 604886, 604901, 604925. Nonsense mutation around 604184, 604724, 604886, 604901, 604925.", "psc": { "uniref90_id": "UniRef90_P75285", "product": "Uncharacterized protein MPN_502", "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75285", "UniRef:UniRef50_P75571" ] }, "pscc": { "uniref50_id": "UniRef50_P75571", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75571" ], "product": "Uncharacterized protein MPN_205" } }, "id": "GIHJHMOALN_1074", "locus": "GCA009939745_01070" }, { "type": "cds", "sequence": "contig_1", "start": 604943, "stop": 605197, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGAGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAA", "aa": "MLQTTGFFNPRRHPEWFDGGQTVADNEKTGFDVDNSENTKQGFQKEADSDKSAPIALPFEAYFANIGNLTWFEQALLVFGICLS", "aa_hexdigest": "451778bce0a189a6b072b668f7a1372b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 77, "aa_cov": 0.9166666666666666, "hmm_cov": 0.6623376623376623, "evalue": 1.9778957158717566e-17, "score": 58.95265197753906, "start": 4, "stop": 80 } ], "seq_stats": { "molecular_weight": 9421.290499999997, "isoelectric_point": 4.418060874938965 }, "id": "GIHJHMOALN_1075", "locus": "GCA009939745_01071" }, { "type": "cds", "sequence": "contig_1", "start": 605342, "stop": 605806, "strand": "+", "frame": 2, "gene": null, "product": "Putative MgpC-like protein MPN_366", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415", "UniRef:UniRef90_A0AB38W808" ], "nt": "GTGGAGGATACCAAGTTGGTGGCGCTGGTCCGTTCAGCGTTGGTCAAGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACTTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGA", "aa": "MEDTKLVALVRSALVKVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPKATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYKTLQDFLVEQPVTPYTPNAGLARVNGVAQDKVHGLEQTTQ", "aa_hexdigest": "7286dd7ec89d30fb731a7c766a8c771c", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 605291, "stop": 605978, "observations": { "insertion": [ 605921 ], "mutation": [ 605333, 605804, 605912 ], "stop": [ 605333, 605804, 605912 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W808", "query_cov": 0.948051948051948, "subject_cov": 0.6160337552742616, "identity": 0.966, "score": 269.0, "evalue": 1.25e-89, "gene_start": 5, "gene_end": 150, "reference_start": 21, "reference_end": 166, "reference_sequence": "MRAAHLHTSYELVDWKVGQNKLVALVRSALVRVKFQDSSSQQGTESNGQDQNALSFDTTKSQQALQQAGDQSGTSSNSTQEDFASYILIFKAAPRATWVFERKIKLALPYVKQESQGSDDQGSNGKGSLYTTLKDLLVEQPVTPYTPNAGLARVNGVAQDKVHGLESGWDNQRSQKNLTNNPGPKAVTGFKLDKGRAYRKLNESWPVSNPSIRPRLARGKVPQNEKRRRQMLRVMLP" }, "paralog": false, "identity": 0.8508771929824561, "score": 364.0, "evalue": 1.9e-129, "description": "Internal stop codon at 605333, 605804, 605912. Frameshift due to insertion around 605921. Nonsense mutation around 605333, 605804, 605912.", "psc": { "uniref90_id": "UniRef90_A0AB38W808", "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W808", "UniRef:UniRef50_P75415" ] }, "pscc": { "uniref50_id": "UniRef50_P75415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75415" ], "product": "Putative MgpC-like protein MPN_366" } }, "id": "GIHJHMOALN_1076", "locus": "GCA009939745_01072" }, { "type": "cds", "sequence": "contig_1", "start": 606017, "stop": 606244, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCTCAACCGAGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGA", "aa": "MSSTESQMAAVTDSQQSGHNSGLVSLAQRSTTVAVQKSDSSGSQGQGTTDNKFQKYLNTAQALHQMGVIVPSLET", "aa_hexdigest": "01a905cfd228e3b6c2671a39b4f01a08", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7844.479600000001, "isoelectric_point": 5.985339164733887 }, "id": "GIHJHMOALN_1077", "locus": "GCA009939745_01073" }, { "type": "cds", "sequence": "contig_1", "start": 606503, "stop": 607060, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21", "UniRef:UniRef90_UPI001FCE6DDF" ], "nt": "TTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCTTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCAAAGATTGCCTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTTGA", "aa": "MNWGTDKQKQFVENQLGFKDDSNSSLTNFKSQGLTQPAYLIAGLDVVQDHLVFAAFKAGAVGYDMTTDSNASTKDQALAWSTTAGLDSAGGYNNLVENTAGLNGPINDLFTLLDTFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGDKKAIYPEVRGRQLQNIF", "aa_hexdigest": "e3d23938cf56cea06e957840f36290b2", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FCE6DDF", "query_cov": 1.0, "subject_cov": 0.8409090909090909, "identity": 0.9890000000000001, "score": 367.0, "evalue": 5.03e-128, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI002246AD21" }, "pscc": { "uniref50_id": "UniRef50_UPI002246AD21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002246AD21" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "GIHJHMOALN_1078", "locus": "GCA009939745_01074" }, { "type": "cds", "sequence": "contig_1", "start": 607466, "stop": 607576, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAGAAAATTTCCGAAAAGGAATACAAAGCTTTAATCCGTAAAACTGGCAAAGAGCACTTTGATGGTGAAAAAGAAGAGTATGGTGATGGCACAGTTGGAGTTTGA", "aa": "MKEKISEKEYKALIRKTGKEHFDGEKEEYGDGTVGV", "aa_hexdigest": "435a21db0dde7c895a3c3aec2dc0ec06", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4130.589700000002, "isoelectric_point": 5.72024211883545 }, "id": "GIHJHMOALN_1079", "locus": "GCA009939745_01075" }, { "type": "cds", "sequence": "contig_1", "start": 607613, "stop": 607846, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAAAGTTAAATACGTAACCCAAGAACAATTTCAGGAATATAAAGATTCCAACAATCAGCGACTCATAAAGATTGAAAATAAGGTTGATAAATTAGTTGAAATTGTTCAAATTCACGGCGAACAAATTAAAGCACAGGGAGAAACCCTGCAATTAATTTTGCAAACGCTGCAAAAGATGAGTGATCGCTTGGACAAGATGGAAAAAAGAATAGACAAACTTGAGTCAAAATAA", "aa": "MKVKYVTQEQFQEYKDSNNQRLIKIENKVDKLVEIVQIHGEQIKAQGETLQLILQTLQKMSDRLDKMEKRIDKLESK", "aa_hexdigest": "21002b18daba759e88e7f408c2bc702c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 88, "aa_cov": 0.8701298701298701, "hmm_cov": 1.0, "evalue": 4.0040556429707194e-20, "score": 67.77593231201172, "start": 2, "stop": 68 } ], "seq_stats": { "molecular_weight": 9203.579399999995, "isoelectric_point": 8.89162464141846 }, "id": "GIHJHMOALN_1080", "locus": "GCA009939745_01076" }, { "type": "cds", "sequence": "contig_1", "start": 608070, "stop": 608312, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATACCAAAGTTTTAAGGGTTTGTCAATTGAAGCTAAGTTGAGCTATAACTTTAATTGGTTTGGAGATTATTCTTCAGGTGGTTTCACTGCTAAGAAAGGTGACAAGCATTATTTTGATCTTTTCCTTAAAATTAAATCTAATTCTGATCCAAAAAAACAATTTACTACAGAAAAATTTCTTACTAAAGAAGAAAAAAATAAAGTTAATGGAAAAGAAATTACAAGAAATTTAGAGTGA", "aa": "MTYQSFKGLSIEAKLSYNFNWFGDYSSGGFTAKKGDKHYFDLFLKIKSNSDPKKQFTTEKFLTKEEKNKVNGKEITRNLE", "aa_hexdigest": "87b6dd737675d38b306775e92f2c9848", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9344.441899999996, "isoelectric_point": 9.456109809875489 }, "id": "GIHJHMOALN_1081", "locus": "GCA009939745_01077" }, { "type": "cds", "sequence": "contig_1", "start": 608427, "stop": 608675, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75316", "UniRef:UniRef90_P75316" ], "nt": "ATGCGCAAAAAGAGATTGTTGTCAAGGATTTCCTTTAGTTCTCTATTTTTATTATGTGGAACAGTTTTAAGCGCCTATACTGGCATCCAAGCAGATCTCAGAAATCTTATCAAGGAAACCACCAAAAAAGACATTGATGTTTACAAGGCAATTAAAACTACAGAAGGAAAAAAGAACATAATAACTTCTTTAAAGAAATCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCCTGA", "aa": "MRKKRLLSRISFSSLFLLCGTVLSAYTGIQADLRNLIKETTKKDIDVYKAIKTTEGKKNIITSLKKSYEVNPKDTTKLLLDA", "aa_hexdigest": "ed29bf6947df9f89923155db9a0c3d5a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 1.0, "subject_cov": 0.8118811881188119, "identity": 0.902, "score": 137.0, "evalue": 1.98e-40, "valid": true, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "GIHJHMOALN_1082", "locus": "GCA009939745_01078" }, { "type": "cds", "sequence": "contig_1", "start": 608695, "stop": 608799, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAAATTTCAGTTTTTTAATTAATGTTCAAACCGAAATTTCAATTAGCTTTCCGTTTTTAAAGTTGCTTTTCCAAAAAAAAAAAAAAAACAAAAATTAA", "aa": "MIENFSFLINVQTEISISFPFLKLLFQKKKKNKN", "aa_hexdigest": "62fa2233dff43946a889b13e31954269", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4088.8537000000015, "isoelectric_point": 10.000287437438963 }, "id": "GIHJHMOALN_1083", "locus": "GCA009939745_01079" }, { "type": "cds", "sequence": "contig_1", "start": 609111, "stop": 609317, "strand": "+", "frame": 3, "gene": null, "product": "Family K-like protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640D", "UniRef:UniRef100_A0AAP8E4R5", "UniRef:UniRef50_A0AAP8E4D4", "UniRef:UniRef90_A0AAP8E4D4" ], "nt": "ATGATAGAAGTATTAAGTGATTCTGAAATTTTGTCTTATTTATTAAGTAATAAGCACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAG", "aa": "MIEVLSDSEILSYLLSNKHTADGVNEIYVTMNIKDKDSNTQRKPDKMSLELELFLNQFIEFLKEQRKS", "aa_hexdigest": "71d7b59af7957e717843d8a92e09190f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD640D", "uniref100_id": "UniRef100_A0AAP8E4R5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD640D", "UniRef:UniRef100_A0AAP8E4R5" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E4R5", "uniref90_id": "UniRef90_A0AAP8E4D4" }, "psc": { "uniref90_id": "UniRef90_A0AAP8E4D4", "product": "Family K-like protein", "uniref50_id": "UniRef50_A0AAP8E4D4" }, "pscc": { "uniref50_id": "UniRef50_A0AAP8E4D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAP8E4D4" ], "product": "Family K-like protein" }, "genes": [], "id": "GIHJHMOALN_1084", "locus": "GCA009939745_01080" }, { "type": "cds", "sequence": "contig_1", "start": 609599, "stop": 610402, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MPN_146", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140" ], "nt": "ATGAAATTTAAGTATGGTGCCATCTTTTTTAGTGGCTTTCTAGGTCTTTCTGCCATTTTGGCTGCCTGTGGTACAAAGGGTAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTCCTCTTTAACTTCTAAAAGTGCATCAAAAGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCCTATTGAAATTACCCAAATCGCTGGTGGTTATGATGGCGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAAGACACCACTAACTTTTACAACTTAATCTTAAACTACCCTGATCTAGTTTCAACTTTAGGTCGTGTTGGTATGGAACTGCCGTTTGACAATGTAAAGGTTGACAAACTATCACCCCGTTTTTTAGATTTCAACAACCGCATTAGTGCAATTTCTAAACCAGGAATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAACAACGCTAAAAAGAAAGAAGGTACTTTAAACCAAAAAATGACAAGTTCTTCTGAAGGAAAAAACAGCAGTGGCACTTTAACAGTAGCAACTGATACTGAAACAAGTAGTTTATGGAAAAAGATAGAAGATTCTGCAAAAGCTAACGGTAAAAGCGATGAAAAAGGAAAAGGTAAGAAGAAAGATAATAAAAGTGCAACTTTTTCGCTTGTACAACTAAAACAAACTCAAGAAAAAACAGATGATTCCCAAGACACTAAAAATAGTGATGATCAAGTTAAAAAATCTTGA", "aa": "MKFKYGAIFFSGFLGLSAILAACGTKGKFDQVDDGKIKLASSLTSKSASKALQAIVKKYNEVKKPGDYPIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELPFDNVKVDKLSPRFLDFNNRISAISKPGIYGIPVSLSTEVLSINGPVLHYILNNAKKKEGTLNQKMTSSSEGKNSSGTLTVATDTETSSLWKKIEDSAKANGKSDEKGKGKKKDNKSATFSLVQLKQTQEKTDDSQDTKNSDDQVKKS", "aa_hexdigest": "3e99355df59412f73486d842bc3f3442", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75140", "query_cov": 1.0, "subject_cov": 0.9434628975265018, "identity": 0.539, "score": 242.0, "evalue": 2.49e-76, "valid": false, "product": "Uncharacterized protein MPN_146", "uniref50_id": "UniRef50_P75140" }, "pscc": { "uniref50_id": "UniRef50_P75140", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75140" ], "product": "Uncharacterized protein MPN_146" }, "genes": [], "id": "GIHJHMOALN_1085", "locus": "GCA009939745_01081" }, { "type": "cds", "sequence": "contig_1", "start": 610592, "stop": 611596, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P75280" ], "nt": "TTGTTTACCTCTGTTTTTGCAGCTGGCGGCGGGGATTACAATAATTTCTTTTACAAGATTGAAAATGGTCGCGCTGACTTTAGTAACTTTAAGAATAAAGGGACTTCTTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTCCAAAAGTTCCACCAATTAGCTTACAGCATTTCCTCTACTTCCGGTTTCTTCTATTCGTTTGCCGGTAAAAGTGCAAAGCGTTTAAATTTTGGTGATAGCTTTATTGAGTATCCGCGGTTTACTCAAGAAATTAAAGCACCATCAAAAAATGGAGAAAATGGCCAAACAAATGAAGGAAATAGTACCAATGGCGAACAAAATCTCTTAGGAACTTTCGAAGTCAAGGATGACAGTAAGCCTAAAGAAGAAGTTAAAAGTAATAAAAATAGTGGTAAAGAAAGTAGCCAAAATCAGGGTAAAAAATCTAACAATAACAAGACTATTTACCTTTATGAAACAAAAATTCCAGATGGAAAAACTGCAGGTGATAATGCTATCTTAATCAAAGATAAGAACGTTATCGAAAAGCTTAAAAGTGCAGCTAAAGAAGAAAATAAAGAACAAACTGCTGAAGCAACTAAAGCTGCAATTACAAGTAATAAAGCAAAAAGCACTAAAAAAGAAAGCAGTAAAGTTATCGGTTACACCACAACTGATAGTGTTCGCGAAGATGGCAAAAACATTTTCGCAATAGATAGAGTTAATGGTGAAAACTATGACCGTAAGATTATTGTAGGTGCTAAAGCAGAAACTTTAAACCAATCTAGCACCTTACAAAGTGAAGAAGCGATTGTTTTGCCTGCCCCTGGAAAGTATCTAAATGGTGATCCCAAGAAAGTGACAATTACCCAAGGACCTAACATCATTGGCATTCATGCTAACGAAAAGGAAAATGCAGAAACGCAAAAGTTCGTAGATTGA", "aa": "MFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGKSAKRLNFGDSFIEYPRFTQEIKAPSKNGENGQTNEGNSTNGEQNLLGTFEVKDDSKPKEEVKSNKNSGKESSQNQGKKSNNNKTIYLYETKIPDGKTAGDNAILIKDKNVIEKLKSAAKEENKEQTAEATKAAITSNKAKSTKKESSKVIGYTTTDSVREDGKNIFAIDRVNGENYDRKIIVGAKAETLNQSSTLQSEEAIVLPAPGKYLNGDPKKVTITQGPNIIGIHANEKENAETQKFVD", "aa_hexdigest": "f7e6ece2e523049b72374748f2386385", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 610292, "stop": 611899, "observations": { "mutation": [ 610400, 610469, 611594, 611618 ], "stop": [ 610400, 610469, 611594, 611618 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75280", "query_cov": 1.0, "subject_cov": 0.41802252816020025, "identity": 0.997, "score": 617.0, "evalue": 2.52e-215, "gene_start": 1, "gene_end": 334, "reference_start": 338, "reference_end": 671, "reference_sequence": "MKFKYCAIFFSGFLGLSAILAACGARGKFDQVDDGKIKLAFSLTSKSASNALQAIVKKYNEVKKPGDYHIEITQIAGGYDGGRSDLQTRVNVKDTTNFYNLILNYPDLVSTLGRVGMELLFDKVNTDKLSDRFLDFNKRISAISKPGIYGIPVSLSTEVLSINGPVLHYILKSAKGDSDNVKVSQRTGETTQKSKVTNPLKINTQNDPATKDLWEKIEASAKANGKSNKDGQTKGKKKVEKSSSSSLVNLKQSTDQTTTDDGSQKSDNKIKESWGEYQEVDGGLKNFEFKASIFENWHDLLDFSTRVAKSFKKIHENSNKKGNDIQGILGVDSTPNSLFTSVFAAGGGDYNNFFYKIENGRADFSNFKNKGTSYQNLQKVFGDFKGLIDKNGIFVNKGGSYSSNFQKFHQLAYSISSTSGFFYSFAGKSAKRLNFGDSFIEYPRFTQEIKAPSKNGENGQTNEGNSTNGEQNLLGTFEVKDDSKPKEEVKSNKNSGKESSQNQGKKSNNNKTIYLYETKIPDGKTAGDNAILIKDKNVIEKLKSAAKEENKEQTAEATKAAITSNKAKSTKKESSKVIGYTTTDSVREDGKNIFAIDRVNGENYDRKIIVGAKAETLNQSSTLQSEEAIVLPAPGKYLNGDPKKVTITQGPNIIGIHANEKENAETQKFVDWFLNSPQTWEKQSRDKKGSSEKQTAAEFFAESASYILPLKEIFDKNDTKTEKGKNSKTQQRTNTYAEKALELFKQISQNQIVSYSDPSDFRSGKFRDAIGATFNAAVSSKADFNKFVQNFTATLGSDI" }, "paralog": false, "identity": 0.9495327102803738, "score": 981.0, "evalue": 0.0, "description": "Internal stop codon at 610400, 610469, 611594, 611618. Nonsense mutation around 610400, 610469, 611594, 611618.", "psc": { "uniref90_id": "UniRef90_P75280", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75280", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "GIHJHMOALN_1086", "locus": "GCA009939745_01082" }, { "type": "cds", "sequence": "contig_1", "start": 611801, "stop": 611980, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A45C19C" ], "nt": "TTGGAACTCTTCAAACAAATTAGTCAAAATCAAATAGTAAGCTACAGTGATCCAAGTGACTTTAGGAGTGGAAAATTCCGTGATGCAATTGGTGCTACTTTCAATGCTGCAGTTAGTTCCAAAGCAGACTTCAATAAATTCGTCCAAAACTTTACCGCTACATTAGGTAGTGACATTTAA", "aa": "MELFKQISQNQIVSYSDPSDFRSGKFRDAIGATFNAAVSSKADFNKFVQNFTATLGSDI", "aa_hexdigest": "5c0aacd6d3654e50d73802bf158724bc", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A45C19C", "query_cov": 0.9152542372881356, "subject_cov": 0.9473684210526315, "identity": 0.815, "score": 92.4, "evalue": 1.1e-23, "valid": false, "product": "Putative mycoplasma lipoprotein, C-terminal region", "uniref50_id": "UniRef50_UPI000A45C19C" }, "pscc": { "uniref50_id": "UniRef50_UPI000A45C19C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A45C19C" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6512.145100000002, "isoelectric_point": 5.987158012390137 }, "id": "GIHJHMOALN_1087", "locus": "GCA009939745_01083" }, { "type": "cds", "sequence": "contig_1", "start": 612100, "stop": 612279, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGCAAATTAGGACTTACAAAATCAAAGATATTTGCGACATTCAACGTGGTCGTGGTATCACTAAGGAATACATTAAAAATAACTCTGGAAAATATCCTGTTTATTCTGCCGCTACTACCAATAACGGTGAGTTAGGTTTTATTAATACTTACGATTTTGCTGGTGAATATGTTACCTGA", "aa": "MQIRTYKIKDICDIQRGRGITKEYIKNNSGKYPVYSAATTNNGELGFINTYDFAGEYVT", "aa_hexdigest": "c88d0c6f6d89cfd8664377c21c3e12f3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 59, "aa_cov": 0.9322033898305084, "hmm_cov": 0.9830508474576272, "evalue": 3.587493412712404e-12, "score": 42.29743957519531, "start": 5, "stop": 59 } ], "seq_stats": { "molecular_weight": 6731.4927000000025, "isoelectric_point": 8.786992454528807 }, "id": "GIHJHMOALN_1088", "locus": "GCA009939745_01084" }, { "type": "cds", "sequence": "contig_1", "start": 612463, "stop": 613101, "strand": "+", "frame": 1, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF507P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_P75279", "UniRef:UniRef90_P75279" ], "nt": "GTGGTAGCTGAAATCTCCTTAGATTTTCCACCTCTAGAAGTTCAAGAAAAAATTGCTCATTTTCTCAAATCTTTTAATGAATTAAGCAGCCAGTTAAAAGCTGAATTAATTAAACGACAAAAGCAATACGCATTTTACAGTGATTATCTACTCAATCCTAAACATTCACAAGGTGAAGAATACAAACTCTTTAAACTCAAAGATATAGCTAAAAAGATTTTAGTTGGCGGTGAAAAGCCAAGTGATTTCCAAAAAGAAAAGGATCAAGTTTATAAATACCCTATTCTTTCTAATAGTCGCAAAGCAGACGATTTTTTAGGCTACAGCAAAACGTTTAGAATTGCTGAAAAATCAATTACAGTTAGTGCAAGAGGCACAATTGGTGCTGTTTTTTACCGTGATTTTTCCTACTTACCCGCTGTTAGTCTAATTTGCTTTATTCCTAAGCCAGAATTTAATATTAAGTTTTTGTTTCATGCTTTAAAAGCGACTAAATTCCACAAACAAGGTTCTGGCACAGGACAGTTAACCATGGCACAGTTTAAAGAATATCAGGTTTATATTCCATCTTTAAAAAAACAACAAGAAATTGCTGCCACTTTGGATCCACTTTACTATATCTTTGCAAATTCTAATTGA", "aa": "MVAEISLDFPPLEVQEKIAHFLKSFNELSSQLKAELIKRQKQYAFYSDYLLNPKHSQGEEYKLFKLKDIAKKILVGGEKPSDFQKEKDQVYKYPILSNSRKADDFLGYSKTFRIAEKSITVSARGTIGAVFYRDFSYLPAVSLICFIPKPEFNIKFLFHALKATKFHKQGSGTGQLTMAQFKEYQVYIPSLKKQQEIAATLDPLYYIFANSN", "aa_hexdigest": "5d4b5140063308675344e44d8f8391b1", "start_type": "GTG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 612163, "stop": 613191, "observations": { "mutation": [ 612277, 613099, 613168 ], "stop": [ 612277, 613099, 613168 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75279", "query_cov": 1.0, "subject_cov": 0.5840220385674931, "identity": 0.991, "score": 412.0, "evalue": 4.94e-143, "gene_start": 1, "gene_end": 212, "reference_start": 122, "reference_end": 333, "reference_sequence": "MQIRTYKIKDICDIQRGRGITKEYIKNNSGKYPVYSAATTNNGELGFINTYDFAGEYVTWTTNGYAGVVFYRNGKFSASQDCGVLKVRNKEINAQFLAFALSLKTPQFVHNLGSRPKLNRKVVAEISLDFPPLEVQEKIAHFLKSFNELSSQLKAELIKRQKQYAFYSDYLLNPKHSQGEEYKLFKLKDIAKKILVGGEKPSDFQKEKDQVYKYPILSNSRKADDFLGYSKTFRIAEKSITVSARGTIGAVFYRDFSYLPAVSLICFIPKPEFNINFLFHALKATKFHKQGSGTGQLTMAQFKEYQVYIPSLKKQQEIAATLDPLYYIFANSNWGIYKEIELRKKQMQYYQERLFQWIENQKV" }, "paralog": false, "identity": 0.9853801169590644, "score": 669.0, "evalue": 7.41e-247, "description": "Internal stop codon at 612277, 613099, 613168. Nonsense mutation around 612277, 613099, 613168.", "psc": { "uniref90_id": "UniRef90_P75279", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF507P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75279", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75279", "EC:3.1.21.3", "UniRef:UniRef50_P75279", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75279", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75279" ], "product": "Putative type I specificity subunit S.MpnORF507P" } }, "id": "GIHJHMOALN_1089", "locus": "GCA009939745_01085" }, { "type": "cds", "sequence": "contig_1", "start": 613219, "stop": 613485, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAACTTAAAGTTCATTGTTGATGGATCAATTCCAGTTTACACCGTTGCTAAAAAAGACTATCCTGGTTCTCTCTTCCAGTTTAATGACAAGGATGAATTGTTATTTACATTGTATGTCAAAGAACAGATTTCATTTGCCGATCCAAACTTTATGAATTTATTGAGAGGACAAAACTTGCATGATTTAGAACTAGTCACCGGAGCTACCAAACCACCGGTAGTTGATCTAGCCAGCTATTTACACTTTGTTCTTTTAAGCGCTTAA", "aa": "MNLKFIVDGSIPVYTVAKKDYPGSLFQFNDKDELLFTLYVKEQISFADPNFMNLLRGQNLHDLELVTGATKPPVVDLASYLHFVLLSA", "aa_hexdigest": "c229286079eed9429ab7835a6239f359", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 47, "aa_cov": 0.5340909090909091, "hmm_cov": 1.0, "evalue": 4.1683546787886167e-17, "score": 58.00649642944336, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 9936.369199999992, "isoelectric_point": 5.066252708435058 }, "id": "GIHJHMOALN_1090", "locus": "GCA009939745_01086" }, { "type": "cds", "sequence": "contig_1", "start": 613729, "stop": 613902, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCAGATAGAAACAGAACTTTCAGTTTCTTAATTGCTGGATTTCGAACTGCCATTGATTGGTATTACAACGCTAAAAAAGAAAATAACGATGCGAAGCAAAAGGCCTTTGGTAGTCAAGGAATCCAATTCCCTAAAGATGGTTTAAATGGAATTTACATGTCCGATTGA", "aa": "MEADRNRTFSFLIAGFRTAIDWYYNAKKENNDAKQKAFGSQGIQFPKDGLNGIYMSD", "aa_hexdigest": "dfe380c494a1cd9798e2d5462de4b0aa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6541.214400000003, "isoelectric_point": 8.034259605407716 }, "id": "GIHJHMOALN_1091", "locus": "GCA009939745_01087" }, { "type": "cds", "sequence": "contig_1", "start": 613963, "stop": 614310, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATAAGCTAATTTCAAACGGCACAAATGATTTAACTACACTTGCTCAAACTGCTTTAGGTGATTCTTTATTTGATACTAAAGTTAACCTGACAGAATCAGTTATTGAAATAAATAACCAAACAGATGTTGCAACTAAATTTAGGGAAAAAGTACTCAATCCTTTCAAGCAAGAAAGAGAAAAAGCACATGCTGAGCACGTTGAAAAGTTAAGGAAACTAGAAGAGGAGCGTAAACTTCAAGAAGCAGAGGCCAAAGCAAAAGCAGAGGAAGTTAAAAAACTCGAAGCAGAAAGAGAAGCTTTTAACAAATCTCTAACAGCTGCATCAGAATTCAAACAATATTGA", "aa": "MHKLISNGTNDLTTLAQTALGDSLFDTKVNLTESVIEINNQTDVATKFREKVLNPFKQEREKAHAEHVEKLRKLEEERKLQEAEAKAKAEEVKKLEAEREAFNKSLTAASEFKQY", "aa_hexdigest": "feae9dad62df26e7bbb04880e7674d64", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13159.652299999987, "isoelectric_point": 5.972948265075684 }, "id": "GIHJHMOALN_1092", "locus": "GCA009939745_01088" }, { "type": "cds", "sequence": "contig_1", "start": 614356, "stop": 614748, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGAATCAACTTCAAGCAGAAATAAATGCAGCTAATGCTGTGTTTCCTGTTTCCGACAGCACTAAAATTCCTAAAGTGTCACAAAAGCTTTTTGGTTTACTTGGTGATGGTTTCTTTCCGCAATTACATCCAAAGGGTTTAAAGATTGCTGACAATATTGCTGCATTATTCGATCAATATAACCTTAAGTCGATTGCACTCAAAAACTTTGATTTAAATTTAGAGCGAAAAAACGACATAGTCATTCAGGGCAAAGTTTGTTATAGTTTTTCCATCAAAATGGATTTTGCAACTATTTATGAAGGTGATGGTAGCACGATAGATTTACAGTTTGCACTCAACGCATCCACTACTAATTTTGCCAATCTAACAGATTTACAAGACAGTTTCTGA", "aa": "MNQLQAEINAANAVFPVSDSTKIPKVSQKLFGLLGDGFFPQLHPKGLKIADNIAALFDQYNLKSIALKNFDLNLERKNDIVIQGKVCYSFSIKMDFATIYEGDGSTIDLQFALNASTTNFANLTDLQDSF", "aa_hexdigest": "cac3197d6f4b36faea8ace113ed0891f", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 134, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.4631827928708315e-43, "score": 143.6189727783203, "start": 1, "stop": 130 } ], "seq_stats": { "molecular_weight": 14356.163999999977, "isoelectric_point": 4.924496269226074 }, "id": "GIHJHMOALN_1093", "locus": "GCA009939745_01089" }, { "type": "cds", "sequence": "contig_1", "start": 614878, "stop": 615129, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGCAACTAAAAAAATTCGCTTCAAAACAAAAGCGTTTTTGCTGGCTTTTTGTACCACTACTGTAACTTTAGGCGCATTTTTATTTCCTACATTAACTCAAAAAGGTTCTAGTGCTGACATTAATACTTTAGCACTACACAATAAAAGTTTAGCTAGAGCCCATAATGACAGCTACTTGGTCAATCTTGCTAACGCATCTACTTTTAGTGAGGAAGAGTTAGCTGTAATAGCGAAAAATAAGGATTGA", "aa": "MKATKKIRFKTKAFLLAFCTTTVTLGAFLFPTLTQKGSSADINTLALHNKSLARAHNDSYLVNLANASTFSEEELAVIAKNKD", "aa_hexdigest": "ca99b181c10a4704c824a4da2ae646e7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9063.374699999995, "isoelectric_point": 9.666728019714355 }, "id": "GIHJHMOALN_1094", "locus": "GCA009939745_01090" }, { "type": "cds", "sequence": "contig_1", "start": 616430, "stop": 616693, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "TTGTTTAATGACTACAACTTAAATTCAATTAAGTTAAAAGCGTTTAACTTGCAGTTGCTCAAACAAAACGACATTGTTTTAAAGAACAAGGTGCGCTATAGTTTTGCCATCGAAATGAAATTTCACACCGTTTATGTTGGCAAAAATACCAAAATTGACCTTGATTTTGCTCTGCAAGCTCAAACGAATAACTTTAGCTCTTTAGAGGAATTACGTGAAAGTTTTACTAATAGTGGTCAAACACTCTCAACCCAATTGTTCTGA", "aa": "MFNDYNLNSIKLKAFNLQLLKQNDIVLKNKVRYSFAIEMKFHTVYVGKNTKIDLDFALQAQTNNFSSLEELRESFTNSGQTLSTQLF", "aa_hexdigest": "252eb302644e232664999629963a48f0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 87, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.97570171113824e-29, "score": 98.10533142089844, "start": 1, "stop": 87 } ], "seq_stats": { "molecular_weight": 10130.437499999993, "isoelectric_point": 9.004250907897951 }, "id": "GIHJHMOALN_1095", "locus": "GCA009939745_01091" }, { "type": "cds", "sequence": "contig_1", "start": 617559, "stop": 617801, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "TTGGCTAACTGGGGTCTTGGTGGCATTCCGCCAGAATGGAGTGGTGACATGGAATTGAAGTTCCAAATTGATGGTAAGCTAGCTAACTGGATTACCCAAAAGCAAGATTACCCTGGTTCGTTATTCCAATTTCAAAATGACAAACTGTTGTTTACCCTACACGTAGTGCAACGTATTACCGTTAAGGATCAACGTTTCATGAACCTGCTAAAGAAACAGCAATTGGATGTGATTGAACCTTGA", "aa": "MANWGLGGIPPEWSGDMELKFQIDGKLANWITQKQDYPGSLFQFQNDKLLFTLHVVQRITVKDQRFMNLLKKQQLDVIEP", "aa_hexdigest": "16af518b765bf6177910c10238d12513", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 59, "aa_cov": 0.725, "hmm_cov": 1.0, "evalue": 2.3173501901871558e-27, "score": 91.21879577636719, "start": 6, "stop": 63 } ], "seq_stats": { "molecular_weight": 9347.710299999997, "isoelectric_point": 6.54025821685791 }, "id": "GIHJHMOALN_1096", "locus": "GCA009939745_01092" }, { "type": "cds", "sequence": "contig_1", "start": 618309, "stop": 618611, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "GTGGTAGAACAATTAACCACAGATGGTGGCAATGATCTTACCAACATTGCTAAAACAGCAATTGGTGAAAGTTTGTTTAACCTCAAGGTTAACTTAACTGATTCAATAATTGATCAAAGCGTTTTACAGCAAGCGCAAAAATCATTTGAGGACAAGATCTTAGATCCGTTCCACGCTGAACGAGTTGAAGCTAAGCGCATTCATGATGAAGAAGCAAGACGCTTAGAGGCGGAACGAAAACGGATTGCAGCTGAATTGAAAGCCAAGGAAGATGAAGCTCGTCGTATTCGTGAAGAACATTGA", "aa": "MVEQLTTDGGNDLTNIAKTAIGESLFNLKVNLTDSIIDQSVLQQAQKSFEDKILDPFHAERVEAKRIHDEEARRLEAERKRIAAELKAKEDEARRIREEH", "aa_hexdigest": "b54a6cae6e0e8032f9d0c95e6e56fd6f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 36, "aa_cov": 0.36, "hmm_cov": 0.9722222222222222, "evalue": 2.9575107058013825e-08, "score": 28.79109764099121, "start": 2, "stop": 37 } ], "seq_stats": { "molecular_weight": 11482.709799999988, "isoelectric_point": 5.236599159240721 }, "id": "GIHJHMOALN_1097", "locus": "GCA009939745_01093" }, { "type": "cds", "sequence": "contig_1", "start": 618618, "stop": 618986, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGCTTACTGTCCCTAAAGTGCCGGAAAACTTCTTTAGTTTCTTTCAGGAAGATTACTTTCCGCGGTTAACTCCCCGTGGTTTGAACATTGCCGATAATGTGGCTTCCTTGTTTAATGACTACAACTTAAATTCAATTGACTTTACTGGTTTTAACCTAAAACTGTTGCGCCAAAACGACATTGTTTTAAAGAATAAGGTGCGTTACAACTTCGCTCTTCAAATGGGCTTTGACACGGTTTACGCTGGAAAGAACACCACTATTTTAAAGTTTAGTTTACAAGCCCAAACAATAAACTTCACTAGCTTACAAGAACTACGTGATAGTTTTGAAAACAATGGCAACACTCTTTCAACCCATCTATTCTGA", "aa": "MLTVPKVPENFFSFFQEDYFPRLTPRGLNIADNVASLFNDYNLNSIDFTGFNLKLLRQNDIVLKNKVRYNFALQMGFDTVYAGKNTTILKFSLQAQTINFTSLQELRDSFENNGNTLSTHLF", "aa_hexdigest": "ba99fc209b20e23f6fffaba29767945e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 120, "aa_cov": 0.9754098360655737, "hmm_cov": 1.0, "evalue": 3.3007011908005805e-36, "score": 120.28932189941406, "start": 4, "stop": 122 } ], "seq_stats": { "molecular_weight": 14103.79939999998, "isoelectric_point": 6.551228141784668 }, "id": "GIHJHMOALN_1098", "locus": "GCA009939745_01094" }, { "type": "cds", "sequence": "contig_1", "start": 619174, "stop": 619335, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTACCCACTTTTTCCCAAAATGGCTCAGCTACTGATCCACAAACTGTAAGTTTAAACAGCAGTAGCTTTGAAAAGCATAACAGTAAGAAATTCTTATTGAAGCGCAAAACTGCTTTAAGTAACGCTGAGTATGAAAACCTTTCCAAGCAAACTAACTGA", "aa": "MVPTFSQNGSATDPQTVSLNSSSFEKHNSKKFLLKRKTALSNAEYENLSKQTN", "aa_hexdigest": "09efebef237f494ae6594bddeff84250", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5905.4779, "isoelectric_point": 9.601679420471193 }, "id": "GIHJHMOALN_1099", "locus": "GCA009939745_01095" }, { "type": "cds", "sequence": "contig_1", "start": 619599, "stop": 619739, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAGCCAGAATTCATGGAATTAATTAAGTCACAAAACCTCCATAACTCAGAACTACGTACCGAGTCCACAAAGCCGCCAGTAGTGGATTTAGCGAGTTATTTACACTACCAACTGTTGAAGTTAAATCAACAGTAA", "aa": "MQEPEFMELIKSQNLHNSELRTESTKPPVVDLASYLHYQLLKLNQQ", "aa_hexdigest": "6592421a541ee2e8a427303cebd6bed2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5442.1408, "isoelectric_point": 5.461397361755371 }, "id": "GIHJHMOALN_1100", "locus": "GCA009939745_01096" }, { "type": "cds", "sequence": "contig_1", "start": 619932, "stop": 620381, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_511", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75275", "UniRef:UniRef90_P75275" ], "nt": "GTGCAAGCCTTTAAAGAATACTGGCAAAAGCAAAAGAAAGATGTTACCGATAAAAAGCAGCTGTTAGAAGCTTTAAAACTTTCCTTTGCCAAAGAACAAAATAAGACCTTTGCCTTTTTGATTAAGAACTTCCAGGACGGTATTAGCAATTATTACCCAAATGACCAAGAAGATCAAAGTGAGGCTGCTAAAACTGCTTTTGGTACACAAGGTATCGCCTTTCCCCAATCTGGTTTAAAGGGCATCTTTATGTCGGAATGGTTGCGTAAACAGCTAGGGGAAAAGGCCAAGATTAACTTAGACATAAAGAGCTTAAAGGTAACTGATTCAAAGATAAGTCCTACCATTAAATGGAACAAAGATATCGGTATTAAGCGAAATCAAGACAAACCTTACAACTTCCGCTTTGAGATTGACATTGAATATCAAGGTAATTACAAATTAAGTTGA", "aa": "MQAFKEYWQKQKKDVTDKKQLLEALKLSFAKEQNKTFAFLIKNFQDGISNYYPNDQEDQSEAAKTAFGTQGIAFPQSGLKGIFMSEWLRKQLGEKAKINLDIKSLKVTDSKISPTIKWNKDIGIKRNQDKPYNFRFEIDIEYQGNYKLS", "aa_hexdigest": "551226bacf23f303f751412bc9ba648a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 619629, "stop": 620381, "observations": { "mutation": [ 619754, 619841, 619889, 619934 ], "stop": [ 619754, 619841, 619889, 619934 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75275", "query_cov": 1.0, "subject_cov": 0.573076923076923, "identity": 1.0, "score": 297.0, "evalue": 2.44e-100, "gene_start": 1, "gene_end": 149, "reference_start": 1, "reference_end": 149, "reference_sequence": "MQAFKEYWQKQKKDVTDKKQLLEALKLSFAKEQNKTFAFLIKNFQDGISNYYPNDQEDQSEAAKTAFGTQGIAFPQSGLKGIFMSEWLRKQLGEKAKINLDIKSLKVTDSKISPTIKWNKDIGIKRNQDKPYNFRFEIDIEYQGNYKLSWLEAIIAKFSGIPGEWKGKLNLKFIVDGDLSWEIVQKPDYPGSLFQFDDQKQQLLFKLHVWEKITVQEPEFMELIKSQNLHNLELRTESTKPPVVDLASYLHYQLLKLNQQ" }, "paralog": false, "identity": 0.976, "score": 483.0, "evalue": 7.22e-176, "description": "Internal stop codon at 619754, 619841, 619889, 619934. Nonsense mutation around 619754, 619841, 619889, 619934.", "psc": { "uniref90_id": "UniRef90_P75275", "product": "Uncharacterized protein MPN_511", "uniref50_id": "UniRef50_P75275", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75275", "UniRef:UniRef50_P75275" ] }, "pscc": { "uniref50_id": "UniRef50_P75275", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75275" ], "product": "Uncharacterized protein MPN_511" } }, "id": "GIHJHMOALN_1101", "locus": "GCA009939745_01097" }, { "type": "cds", "sequence": "contig_1", "start": 620412, "stop": 620681, "strand": "-", "frame": 2, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029624419", "UniRef:UniRef90_UPI0029624419" ], "nt": "GTGTTGGGTAATAACAATGATTTAACAACTGTAGCAAAAGCAGCTGTTGGTGATAATTTATTTACCACCCAAGCTAATTTAACCAAGTCAGTTTTGGATCAAACCGTGCTAAAAGAGGCTGAATCAAGGTTTGAAGCAACTGTTTTAAAACCGTTCATTGAAGCACGGCAAAAGGCACTAGCGGAACACCAAGCACACCAAAAAGTCTTAGAGGAGCAACGCCAAAAACAGCTCGAAGAATTAAAACAAAAACAAAAGGAAGCTGAATAG", "aa": "MLGNNNDLTTVAKAAVGDNLFTTQANLTKSVLDQTVLKEAESRFEATVLKPFIEARQKALAEHQAHQKVLEEQRQKQLEELKQKQKEAE", "aa_hexdigest": "15ef2cc78a3a3556ba18ef463dc36d14", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 620463, "stop": 620756, "observations": { "mutation": [ 620705 ], "stop": [ 620705 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029624419", "query_cov": 1.0, "subject_cov": 0.7807017543859649, "identity": 0.9890000000000001, "score": 136.0, "evalue": 7.91e-40, "gene_start": 1, "gene_end": 89, "reference_start": 26, "reference_end": 114, "reference_sequence": "MQDSLVLRGQNLTTQLFWKPTVKRLVLGNNNDLTTVAKAAVGDNLFTTQANLTKSVLDQTVLKEAESRFEATVLKPFIEARQKALAEHQAHQKVLEEQRQKQLEELKQKQKEAE" }, "paralog": false, "identity": 0.979381443298969, "score": 182.0, "evalue": 1.12e-60, "description": "Internal stop codon at 620705. Nonsense mutation around 620705.", "psc": { "uniref90_id": "UniRef90_UPI0029624419", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI0029624419", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029624419", "UniRef:UniRef50_UPI0029624419" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029624419", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029624419" ], "product": "DUF240 domain-containing protein" } }, "id": "GIHJHMOALN_1102", "locus": "GCA009939745_01098" }, { "type": "cds", "sequence": "contig_1", "start": 620850, "stop": 621242, "strand": "-", "frame": 2, "gene": null, "product": "DUF240 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4F51D6", "UniRef:UniRef90_UPI000A4F51D6" ], "nt": "TTGCAAACTAAGTTTAAGGACAATTCGCTTAACCCACAACAGTTGGGTGTGATTGATGTTTACAACCTGTTGAGTGGATTGGAACAAAGTACGCGTGATACCGTTAAGTTAATTAATGACTTAAATGCAAAGGTATTACTAGCAAATGCCGTTTTTCCGGTGCACGAGGCATTTCAATTGCCCCAAGTACCCGAACAGCTGTTTACCATTTTGGGTAGTGATTTCTTTCCTCAATTAACCCCTAAGGGTTTAAATGTGACTGAGAATATTACGACAGTTTTGCAACAATACAAACTTCAAAGCATTGCGTTAAAGCAGTTTGATCTGAAACTAGAAAAACAACCGGACATTGTGAAGCGCTACAAGATGCGTTACAGCTTCGTAATCCAATGA", "aa": "MQTKFKDNSLNPQQLGVIDVYNLLSGLEQSTRDTVKLINDLNAKVLLANAVFPVHEAFQLPQVPEQLFTILGSDFFPQLTPKGLNVTENITTVLQQYKLQSIALKQFDLKLEKQPDIVKRYKMRYSFVIQ", "aa_hexdigest": "02e1bd1b17cff7fe0eb095975645971f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 620671, "stop": 621503, "observations": { "deletion": [ 620765 ], "mutation": [ 620852, 621263, 621416 ], "stop": [ 620852, 621263, 621416 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A4F51D6", "query_cov": 1.0, "subject_cov": 0.4693140794223827, "identity": 0.992, "score": 252.0, "evalue": 8.54e-83, "gene_start": 1, "gene_end": 130, "reference_start": 88, "reference_end": 217, "reference_sequence": "MKLIKKKRLLLKTLSLSLAGLTTTLAIVVWPTLTEKTNQSVSDFTLSNQRFEQNQSNRYQLQFNSGISQADLDYTTKHTNWKANFEQLQTKFKDNSLNPQQLGVIDVYNLLSGLEQSTRDTVKLINDLNAKVLLANAVFPVHEAFQLPQVPEQLFTILGSDFFPQLTPKGLNVTENITTVLQQYKLQSIALKQFDLKLEKQPDIVKRYKMRYSFVIQWDFETVYTGVNSTTNLAFSVKAMTTNFANLTGIARQLGFTWPELNYTTFLKANGQAVSVG" }, "paralog": false, "identity": 0.9855595667870036, "score": 523.0, "evalue": 9.27e-192, "description": "Internal stop codon at 620852, 621263, 621416. Frameshift due to deletion around 620765. Nonsense mutation around 620852, 621263, 621416.", "psc": { "uniref90_id": "UniRef90_UPI000A4F51D6", "product": "DUF240 domain-containing protein", "uniref50_id": "UniRef50_UPI000A4F51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A4F51D6", "UniRef:UniRef50_UPI000A4F51D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A4F51D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4F51D6" ], "product": "DUF240 domain-containing protein" } }, "id": "GIHJHMOALN_1103", "locus": "GCA009939745_01099" }, { "type": "cds", "sequence": "contig_1", "start": 621654, "stop": 622106, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_513", "db_xrefs": [ "RefSeq:WP_010874869.1", "SO:0001217", "UniParc:UPI000013ACFB", "UniRef:UniRef100_P75273", "UniRef:UniRef50_P75273", "UniRef:UniRef90_P75273" ], "nt": "ATGTACGACATGATTAATGCCAAGTACTTGGGCAAGTTAATCTTTTTGGATTTTGGCCAAACAATTACGAGTTTGGAAGAAACGAGTGATAACTCGACCTTTTTTATTGCTGATGAATTTGGTAATTTTGGGAGCCACTATGTGGGGGTGGTATTGGAGCAAGTCCGCTCGTTCAATACTGTAGTTATCCTGTCCTATCAATCATTTGCTAACCTGCGCAATATTGGTGGTGATAACTTTAAATCACAGATTATTAACAACACCAGTACCCTAATTGCCCACCGCTTAATTGACATGATGGAAGCAGAAGAAGTGGCTAACCTGTATGGGGTTGATATTACCTGTGCTAGTGAAATTAAAAGTTTAAAAGTGGGTCAAGCACTAGTGCGCAGTATCTCTAGTTATTATGGCGAAATCAAAGACTGGCTGGTGCAACAAATCGATAAAAGCTAA", "aa": "MYDMINAKYLGKLIFLDFGQTITSLEETSDNSTFFIADEFGNFGSHYVGVVLEQVRSFNTVVILSYQSFANLRNIGGDNFKSQIINNTSTLIAHRLIDMMEAEEVANLYGVDITCASEIKSLKVGQALVRSISSYYGEIKDWLVQQIDKS", "aa_hexdigest": "cb8379474cbdfd0cfb5bb4e6f49746f2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ACFB", "ncbi_nrp_id": "WP_010874869.1", "uniref100_id": "UniRef100_P75273", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ACFB", "RefSeq:WP_010874869.1", "UniRef:UniRef100_P75273" ] }, "ips": { "uniref100_id": "UniRef100_P75273", "uniref90_id": "UniRef90_P75273" }, "psc": { "uniref90_id": "UniRef90_P75273", "product": "Uncharacterized protein MPN_513", "uniref50_id": "UniRef50_P75273" }, "pscc": { "uniref50_id": "UniRef50_P75273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75273" ], "product": "Uncharacterized protein MPN_513" }, "genes": [], "id": "GIHJHMOALN_1104", "locus": "GCA009939745_01100" }, { "type": "cds", "sequence": "contig_1", "start": 622111, "stop": 622374, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014575013.1", "SO:0001217", "UniParc:UPI0001DD6414", "UniRef:UniRef100_A0A0H3DLB1", "UniRef:UniRef50_A0A0H3DLB1", "UniRef:UniRef90_A0A0H3DLB1" ], "nt": "TTGTTTAAAAAAATTCACAAAGAAAAACGCCGAAAAGAAATTTTAGAAATGAAAGAAAAAGGTCGCTTTCAACCTTCCAATCAAATTTTTGTAGAAAAAATTGATGAATACTATACGGAGCATGTTCAAACCATTGACACAAAGGACTTTGAAAATTTAAGAGAAAACCTCAGGGAACTAGCGGAAATTGGTTCAATCGATTCTAATGGTGTGGGATTGATAGATATTATCAAACTGCCAAGATGCTGTTTTGTTTTCCATTAG", "aa": "MFKKIHKEKRRKEILEMKEKGRFQPSNQIFVEKIDEYYTEHVQTIDTKDFENLRENLRELAEIGSIDSNGVGLIDIIKLPRCCFVFH", "aa_hexdigest": "d4a9f5f12a5637ee2633483fdccff83a", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6414", "ncbi_nrp_id": "WP_014575013.1", "uniref100_id": "UniRef100_A0A0H3DLB1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6414", "RefSeq:WP_014575013.1", "UniRef:UniRef100_A0A0H3DLB1" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DLB1", "uniref90_id": "UniRef90_A0A0H3DLB1" }, "psc": { "uniref90_id": "UniRef90_A0A0H3DLB1", "uniref50_id": "UniRef50_A0A0H3DLB1" }, "pscc": { "uniref50_id": "UniRef50_A0A0H3DLB1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0H3DLB1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10427.929099999992, "isoelectric_point": 6.830591773986817 }, "id": "GIHJHMOALN_1105", "locus": "GCA009939745_01101" }, { "type": "cds", "sequence": "contig_1", "start": 622623, "stop": 622985, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_514", "db_xrefs": [ "RefSeq:WP_010874870.1", "SO:0001217", "UniParc:UPI000013ACFE", "UniRef:UniRef100_P75272", "UniRef:UniRef50_P75272", "UniRef:UniRef90_P75272" ], "nt": "ATGGAACAGGACATCAATAACCAGACTGGTAAGAAAATCTTTTTAAACGAGGAGTGTTTTTTGGAACTGGACAAGCTCCCCCAGCACGTCTCGGTACTAGGTGTGAGTGGTTTTGGTAAGTCCAATATCTTGCTGCACTTTTTGAAGTATGCAATTGACAACGACCATCCCTTAATTTTTGTGAATGGTAAGGGTGATAAGGAATTAATTACCCAGTTTGAACACTACCAAACGAGCGCCAAACAACTAAGTCCCGAAGACGGTTTTGATACGGTTAAGTTAGTGGCACTCAAAAATACTAGTGCCAAAATTTGGAGCTTAGATGACAGAATTGCCACGATTAAGTACAACCCGTTTAAGTAA", "aa": "MEQDINNQTGKKIFLNEECFLELDKLPQHVSVLGVSGFGKSNILLHFLKYAIDNDHPLIFVNGKGDKELITQFEHYQTSAKQLSPEDGFDTVKLVALKNTSAKIWSLDDRIATIKYNPFK", "aa_hexdigest": "2b86db1c391cf7a1ecc8ae05bfe76987", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ACFE", "ncbi_nrp_id": "WP_010874870.1", "uniref100_id": "UniRef100_P75272", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ACFE", "RefSeq:WP_010874870.1", "UniRef:UniRef100_P75272" ] }, "ips": { "uniref100_id": "UniRef100_P75272", "uniref90_id": "UniRef90_P75272" }, "psc": { "uniref90_id": "UniRef90_P75272", "product": "Uncharacterized protein MPN_514", "uniref50_id": "UniRef50_P75272" }, "pscc": { "uniref50_id": "UniRef50_P75272", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75272" ], "product": "Uncharacterized protein MPN_514" }, "genes": [], "id": "GIHJHMOALN_1106", "locus": "GCA009939745_01102" }, { "type": "cds", "sequence": "contig_1", "start": 623147, "stop": 624670, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "GTGAAGGAAAAGGTGTCGAGTGAGATCCAGGACTTAATTAAACGTGAAGAGTACCGTGATAACTCGATTGTTGTCATGGCCGATTCCGGGGCGCGGGGTAATATCTCCAACTTTACCCAGCTGTTTGGGATGCGGGGGTTAATGAGTAAGAGCTTTAACTATGAACGCAACAACCAGTCCAAAATTATTAAGGACACGATTGAGGTTCCTATTAAGCACTCCTTCCTGGAAGGTTTAACGATTAACGAGTACTTCAACTCATCTTATGGGGCGCGTAAGGGGATGACCGATACCGCCATGAAAACGGCTAAGTCTGGTTACATGACCCGTAAACTGGTGGATGCAACCCACGAATTGATTATTAACCACGATGAATGTGGTACACGCAAGGGAATTGTGGTCGAAGCAATTGTGGAAACCAAAACCCGTTCGTTGGTGGAATCACTGTTTGACCGCATTGTCAACCGTTACACGATTGGACCAATTTTAGATCCCGAAACCAAGGCGGAAATTGTCCCTGCTAACAGCCTAATTACCCAAGAGTTAGCCAAGCAAATTTGTGCCACTTCGATTAAACAAGTGTTAGTGCGCTCTGTGATTTACTGTGAACGCGAAAACGGCGTGTGTCAGTACTGTTTCGGGGTCGATTTGTCCACCGGCAAACTGGTGGAATTGGGTACCGCTGTGGGTGTAATTGCAGCGCAATCGATCGGTGAACCAGGAACCCAGTTAACGATGCGTACCTTCCACACCGGGGGGGTTTCGACCGAAAACAACTTGGCCCAAGGGTTTGAACGTTTAAAGCAAATCTTTGAAGTAGTCGCCCCTAAGGATTACGAGCGCTGTGTCATTTCTGAAGTTAAGGGGGTAGTTAAATCGATTACTACCACCCAAAACGCGCAGGAAGTGTTGATTGAATCTAGTGTCGATGAACGCACTTACTCGATTCCGTTTAGTGCGCAACTGCGTGTCAAGGTCGGTGATGCAGTAGAACTAGGTTCGAAGATTACCGAAGGTTCGATTGACATTCGCCAGTTATTACGGGTTGCTGGGATCCAACGGGTACGCCAGTACATGATTGTCGAAATCCAAAAGGTGTACCGTATTCAGGGGATTGAGATTGCTGATAAGTATGTCGAAATTATTATCCGCCAGTTGACGAGCTTACTCCAAGTAACCGATGCTGGCAGCAGTAACCTGTTTGTGGGTCAACTGGTACACAGTCACCACCTCAATGAACTCAACAAGAGCTTGTTGCTGTCGGGGAAGATGCCAGTGATTGCCATTAACCAGGTGTTTGGGATCGATGAGGCGGCCAGCAAGTCGAACTCCTTCTTGAGTGCCGCATCCTTCCAAGACACCAAGAAGATCTTGACCGATGCTGCGGTAAAAACCCAAGTTGACTACCTCTTAGGGTTGAAAGAAAACGTGATCATTGGGGGTAAGATCCCGGCTGGTACTGGGTTTTTAACCGATGAAGAATTGGCCTACCTCGGTGCCAAAACGGTTCAAGAGGAGTATTAA", "aa": "MKEKVSSEIQDLIKREEYRDNSIVVMADSGARGNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFLEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDECGTRKGIVVEAIVETKTRSLVESLFDRIVNRYTIGPILDPETKAEIVPANSLITQELAKQICATSIKQVLVRSVIYCERENGVCQYCFGVDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVAPKDYERCVISEVKGVVKSITTTQNAQEVLIESSVDERTYSIPFSAQLRVKVGDAVELGSKITEGSIDIRQLLRVAGIQRVRQYMIVEIQKVYRIQGIEIADKYVEIIIRQLTSLLQVTDAGSSNLFVGQLVHSHHLNELNKSLLLSGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKTQVDYLLGLKENVIIGGKIPAGTGFLTDEELAYLGAKTVQEEY", "aa_hexdigest": "112c9511e2ee87c972bcfaf17e3b675e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 77, "aa_cov": 0.14398422090729784, "hmm_cov": 0.987012987012987, "evalue": 5.2670910198563985e-17, "score": 57.23020553588867, "start": 3, "stop": 75 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 341, "aa_cov": 0.6331360946745562, "hmm_cov": 0.7536656891495601, "evalue": 1.7350707372802482e-58, "score": 193.60702514648438, "start": 78, "stop": 398 } ], "seq_stats": { "molecular_weight": 56015.58430000006, "isoelectric_point": 6.255779075622559 }, "id": "GIHJHMOALN_1107", "locus": "GCA009939745_01103" }, { "type": "cds", "sequence": "contig_1", "start": 624677, "stop": 626860, "strand": "-", "frame": 3, "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "db_xrefs": [ "COG:COG0086", "COG:K", "EC:2.7.7.6", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270", "KEGG:K03046", "SO:0001217", "UniRef:UniRef50_P47582", "UniRef:UniRef90_P47582" ], "nt": "TTGTTTGATGAAGCCATTTTTGGTCCAGTGAAGGACTATGAGTGTGCTTGTGGGAAGTTTAAGAAGATTAAGTACCGTGGCGTGCGCTGTGATCGCTGTGGTGTTTGGGTAACCGAATCAATTGTGCGCCGGGAACGTATGGGCCACATTGCCCTAGTCAGTCCGGTAGCGCACATCTGGATGTCGAAGGAACTGCCTTCCCCTTCCAAGATCTCCTTAGTGTTAAACATCTCTTATAAGGAAGTGGAACAGGTCTTGTACTTTGTTAACTACATAGTATTAGATACTGGGAAGATCAAAGATCCCAAGATCATGCCGTTTAAGTTTAAGGAAGTGTTGGACTTAGCAGGCAAGGGTTCTTTAACCACGCGGCAAAAAATGCGCCGGGTGATCGGTTACATCTTCCGGAACCTGATTAAGAACAGGAGTAGCGAAGACTACCGCAAGGGGAAGATCTTTTATGAAAGCTTAAAGAACAGTTCGTTACCGTTCTCCTTAAATGATGCCTTTAACTACATTAAGAAGTACACTGGCTTCCGCGTTGGTATCGGGGCTGAAGCCATCTTAGAATTGCTCAACAAGATTGACCTTAACTATGAGTTTAGTAAGCTCAATGATGCGTTACGGAAGGCTAAGAAGGATAGTGTTGAGGATGCTAAGGTTAAAAAGATCTTGCGTCAATTGGAAACAATTAGCTGGTTTAGAAATTCGAAGCTGCACCCGAAAAACATGATCCTGCACACGGTGCCAGTGATCCCGCCAGACATCCGGCCGATTATCCAGTTAGATGGTGCTAAATTTACCACCAGTGACATTAATAACTTCTACAGAAGGGTAATTATCCGTAACGACCGTTTACGCCGCATCTTGGAAGATGGTACCGTACCTGCCATTGTGGTTAACAACGAAAAGCGCTTGTTACAAGAATCAGTTGATGCCTTGTTTGACAACTCGTCGCGCCACAAGCCAGCGTTATCCAAAGACAAACGTTCCTTGAAGTCACTCACCGACCGCTTAAAAGGGAAACAAGGGTTGTTCCGTCACAACCTTTTAGGTAAACGGGTGGACTATTCTGGTCGGAGTGTAATTGTGGTGGGTCCAGAACTAAAGATGTATGAGGTCGGGATTCCAGCATTAATGATCCTCAAGCTGTTCAAACCATTTATTATTCACGGGTTAATTAATAAGTTTGACAGCAACGGTAACGAAATTAGACCAATTGCGTCCTCGATTCGGCAAGCGGAGGACATGATTAAAAATCAGGACGACCTGATCTGGGGGATTGTGTATGATGTCATTAAGGACCGTCCGGTGTTATTAAACCGGGCACCTACTTTGCACCGTTTGGGAATACAGGCCTTTGAACCGCGGATTGTCGATGGGAAAGCGATTCGCCTACACCCGTTAGTGACCACTGCTTTTAACGCTGACTTTGACGGTGACCAGATGGCGGTGCACGTTCCGTTGAGCGAAAATGCCGTTAACGAAGCGCGTGCTATTCTGTTAGCTTCCAAGCACATCTTGGGACTAAAAGATGGCCGGCCAATTGTCACCCCAACCCAGGACATGGTGTTAGGTAACTATTACCTCACTACTGAGCGTAAGGGTCAAACGGGTGAAGGCATCATCTTTGGTACCGTGCATGAAGCCCGCGCGGCATACGAAGCGGGCAAGGTGCACCTCCACGCCATTGTCGGCATTAGCACCAAAGCCTTTCCAAACAAGCACTTTGAAGCTCAAGGTACTCTAATCACTACGGTTGGTAAGATTATCTTTAACGATGTGTTAGGGGACAACATCCCTTACATTAATGAAGGGGAATTTGATGAACATGCGTGTCCGCAAAAGTTCATAGTACCACCAAGTGGTGATGTGCGTGCTGCGATTGCCGCACACCAAGTGCTCCCTGCCTTTGGTAAGAAGGTCATCTCCAAGTTAATTGACTTACTTTACACGGTGGTTGAATTTAAGGATCTACCACGGATCTTGGACAACATTAAGGCCTTAGGCTTTAAGTACTCGACCCACTCTTCCACCACTGTATCGGTGTTTGATATTCCGAAGTACTCCAACAAACAGCAGTACTTTGATGAAGCGGACCAACAGGTCTTGAAGTACAAGCAGTTTTACAACAAGGGTTTACTGACCGATGATGAACGGTATAAACGGGTGGTGAAACTGTGA", "aa": "MFDEAIFGPVKDYECACGKFKKIKYRGVRCDRCGVWVTESIVRRERMGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDPKIMPFKFKEVLDLAGKGSLTTRQKMRRVIGYIFRNLIKNRSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNYEFSKLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPAIVVNNEKRLLQESVDALFDNSSRHKPALSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDSNGNEIRPIASSIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSENAVNEARAILLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQTGEGIIFGTVHEARAAYEAGKVHLHAIVGISTKAFPNKHFEAQGTLITTVGKIIFNDVLGDNIPYINEGEFDEHACPQKFIVPPSGDVRAAIAAHQVLPAFGKKVISKLIDLLYTVVEFKDLPRILDNIKALGFKYSTHSSTTVSVFDIPKYSNKQQYFDEADQQVLKYKQFYNKGLLTDDERYKRVVKL", "aa_hexdigest": "63784c63a4ef0c4a102a9a09cd476799", "start_type": "TTG", "rbs_motif": "AAAA", "genes": [ "rpoC" ], "truncated": "both", "pseudogene": { "start": 624374, "stop": 627016, "observations": { "mutation": [ 624679, 626929 ], "stop": [ 624679, 626929 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47582", "query_cov": 1.0, "subject_cov": 0.5626934984520123, "identity": 0.937, "score": 1348.0, "evalue": 0.0, "gene_start": 1, "gene_end": 727, "reference_start": 56, "reference_end": 782, "reference_sequence": "MTTTRRNKRNNKLYKNIKAIKLSIASNDTILNWSEGEVTKAETINYKSLKPEPGGLFDEAIFGPVKDYECACGKFKKIKYRGVRCDRCGVWVTESIVRRERMGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDDKIMPFKFKEVLDLTGKGSLSTRQKMRRVIGYIFRNLIKSKSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNLEFSRLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPSIVVNNEKRLLQESVDALFDNSSRHKPSLSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDENGNEIRPIAASIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSENAVNEARAVLLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQLGEGIIFSTVYEARAAYESQKVHLHAIVGISTKAFPNKKFACQGTLITTVGKIIFNDVLGNNVPYINDGEFDENACPEKFIVKQGEDVRQSILKHQIIPAFSKKVISKLIDLLYLLLEFKDLPKTLDNIKALGFKYSTFSSTTVSVFDIPKYTNKQNYFDSADQQVLKYKQFYNKGLLTDDERYKRVVKLWNNVKEKVSDEIQNLIKQEQYRDNSIVVMADSGARCNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFFEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDDCGTRKGIVVEAIVETKTKSLIESLFDRIVNRYSITPIVDPETQKTIVEANSLITTQLAKQICATSIKEVLVRSVIYCERENGICQYCFGIDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVTPKDFEKAVISEVKGTVKSITTVQNAQEVVIKSNVDERIYTIPFSAQIRVHVGDQVSPGSKITEGSVDIKQLLRIAGIQRVRQYMIVEIQKVYRIQGIDIADKYVEIIIRQLTNLLQVTDAGNSNLFVGQLVHSHYLNELNKSLLLAGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKNQVDYLLGLKENVIIGGKIPAGTGFLTDEELTFLGSKTVAEEY" }, "paralog": false, "identity": 0.9363636363636364, "score": 1637.0, "evalue": 0.0, "description": "Internal stop codon at 624679, 626929. Nonsense mutation around 624679, 626929.", "psc": { "uniref90_id": "UniRef90_P47582", "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47582", "cog_id": "COG0086", "cog_category": "K", "kegg_orthology_id": "K03046", "go_ids": [ "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47582", "EC:2.7.7.6", "UniRef:UniRef50_P47582", "COG:COG0086", "COG:K", "KEGG:K03046", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47582", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47582" ], "product": "DNA-directed RNA polymerase subunit beta'" } }, "id": "GIHJHMOALN_1108", "locus": "GCA009939745_01104" }, { "type": "cds", "sequence": "contig_1", "start": 626927, "stop": 627019, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAGCGTAATAAAAAGAACAACAAGCTGTACAAGAACATTAAGGCAATTAAGCTTTCGATTGCTTCCAACGACACGATCCTAAACTGA", "aa": "MTKRNKKNNKLYKNIKAIKLSIASNDTILN", "aa_hexdigest": "72f98435a436ea482b017ee4a2a46954", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3476.1007, "isoelectric_point": 10.468972587585448 }, "id": "GIHJHMOALN_1109", "locus": "GCA009939745_01105" }, { "type": "cds", "sequence": "contig_1", "start": 627023, "stop": 627319, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04560.25" ], "nt": "TTGGAAGCTTACGGCGCTGCTTACAACCTTCAGGAATTACTTACCATTAAGTCCGATGATGTGCAGGGCCGGAATAAGGCCTATGCCGCGATCGTTAAGGGCGCTGCCTTCCCCGAACCAGGTATCCCCGAATCGTTTAAACTGTTAACGAAGGAACTACAAGGGTTAGCGTTGAGTGTGTCCTTTATTTACGATGACAACACCCAACAAGATTCCAACAACGTTTCAATTCTCCAAGCTGATGGAGAACAGGACGATCTCTTTAATGACTTTGAATTTGACACGGAGGGTTATTAA", "aa": "MEAYGAAYNLQELLTIKSDDVQGRNKAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQADGEQDDLFNDFEFDTEGY", "aa_hexdigest": "f83241a1831aaa94eba3c9c027ad662e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04560.25", "name": "RNA_pol_Rpb2_7", "length": 73, "aa_cov": 0.6428571428571429, "hmm_cov": 1.0, "evalue": 3.4698260567095115e-19, "score": 64.57222747802734, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 10832.72439999999, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_1110", "locus": "GCA009939745_01106" }, { "type": "cds", "sequence": "contig_1", "start": 627323, "stop": 628471, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33" ], "nt": "GTGATTTCCGAGCGCTTAGTGAAGGATGATGTTCTAACCTCCTTAACGATTAACGAATACGTAGCCCAGTGTTTATCGACCAAAAACGGTGATGAACAAATTACCCGTGATATCCCCAACGTTAGTGATGCTAACAAGCGTTACTTAGATGAAAACGGCATTATCATGGTCGGTGCTGAAGTTAAGGAAGGTGATGTCCTTGTCGGTAAGGTATCCCCTAAGGGACAGGTTGAAGTATCACCTGAAGAAAAGCTGTTTAAGGCCATTTTCCCTGAAAGTGTGCAAAACGTCCGTGACTCTTCCTTAAAGCTCCCTCATGGTGGTGATGGGATTGTGTCCTGTGTCAAGCGTTTTAGCATTGCCAACGGTAATGAACTTAATGATGGTGTCATCGAAATGATTAAGGTCTATGTGGTGCAAAAACGCAAGATCCAAATCGGAGATAAGTTGGCAGGGCGCCATGGTAACAAAGGGGTTATCTCTAAGGTTGTCCCAGTAGCGGACATGCCCCACTTAGAAGATGGTACTCCAGTCGACATCCTCTTAAACCCACTGGGGGTACCGAGTCGGATGAATATTGGCCAAATCTTTGAAATGCACTTGGGTTATGCAGCACATAACCTTGCCAAGCGAATGTTAATTAGTGCTTGTTTTGATGACAAGAAGGCTCAAGCACTTTCAACCGAAATTAACCAGCCACAGTACAAACTTGACCGCTTAATTACTGGTTTAAAAGCCCAAATTACGAACCGCGGTCTTAAAGACGAGCAGGCAGCCTTGGCCCAACTCAATAACGGTGACATTGCTTTAGTGCTCAAGGAAATCGGCATGAGCTTTGATGACCTCCACTTTAAAGTAGCGACCCCGATCTTCCAAGGGGTGAACTTCCAAGACTTGCAAGACATCATGGATGAAGCAGGATTGAAACCAGCGGAAACGCACGGGAAGTTCAAGCTGATTGATGGTCGCACTGGTTTACCATTTGAAAAACCAATTTCCTTGGGAATCATGTACATGATGAAGTTAAACCACATGGTTGATGACAAGATCCATGCTCGCGCTGTCGGTCCTTACTCCAAGATTACCCAACAACCATTGGGTGGTAAATCACAAAATGGGGGCCAACGCTTTGGGGAAATGGAAGTGTGA", "aa": "MISERLVKDDVLTSLTINEYVAQCLSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKLPHGGDGIVSCVKRFSIANGNELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPVADMPHLEDGTPVDILLNPLGVPSRMNIGQIFEMHLGYAAHNLAKRMLISACFDDKKAQALSTEINQPQYKLDRLITGLKAQITNRGLKDEQAALAQLNNGDIALVLKEIGMSFDDLHFKVATPIFQGVNFQDLQDIMDEAGLKPAETHGKFKLIDGRTGLPFEKPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEV", "aa_hexdigest": "0313eaaf14e12719edb25ed8f4070b9e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 377, "aa_cov": 0.9712041884816754, "hmm_cov": 0.7559681697612732, "evalue": 9.69991270658408e-101, "score": 332.989501953125, "start": 1, "stop": 371 } ], "seq_stats": { "molecular_weight": 41965.923000000046, "isoelectric_point": 6.332909584045409 }, "id": "GIHJHMOALN_1111", "locus": "GCA009939745_01107" }, { "type": "cds", "sequence": "contig_1", "start": 628499, "stop": 631198, "strand": "-", "frame": 3, "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "COG:COG0085", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549", "SO:0001217", "UniRef:UniRef50_P47715", "UniRef:UniRef90_P78013" ], "nt": "ATGTCACAAAAACCTAGTTTCTTTCAGAAAAAATACTCACCCACTGCTACCCGCCGTTACTATGGCAAAATTGCCACTGATTTTGTGCAACCTAATCTGGCTGATATTCAGATTAGGAGCTATCAAACTTTTCTAGATCACGATCTGGAAAACCTGATTGCTGCTTATTTTCCAATTAAGTCCCCTAATGATCGTTACACGATCAACTTTAAGGGTTTGCGTCGTACCGCTCCTGAACGTAATGAAGCCCAATCACGCTCTGAGTCCAAGACCTATGAAATTGGTATTTACGCTGACTTAGAATTAATTGATTCCGCTACCGGAACCATTAAAAAGCCGCGCAAATCCAAGAAGAACAGTGCCACCAGTAGTGTCGATGGCGTTTTCTTAACTAACTTACCATTGATTACCCGCGATGGGGTCTTTATTGTCAACGGGATTGAGAAGTTTGTGATTGCCCAAATTACCCGTTCACCGGGGATCTACATGCTCACTAAGTCCCAGTTAAAATTGTCTAGTTCGCGTAAACGGGTACAAGAAGGTTATGTATGTGAAGTATTACCAGCCAACGGTTCGGTAATGCTGATCTACATCTCCAATAAAAAGAAGATTGAAGATGCCTTTGTGCAAATCTTGCTCCGGGATGCTGTACGTGAAGGGGCAAAGATCTTCCCGATTACCACCTTGCTCAAAGCCTTTGGGATGAGTGGCAAGGAAATCCTCAAAGTTTTCAAAAACAACGAGTTTATTACCCGTTCCTTAGAAGCGGAAGTCTACAACGCTAAGGATTTCTTAAACAACGTTGACCCTGAAATTAAAAACTTATTAAGGGAATTCCGCGATGGCAAAACTGATCTCCGCCGTAAGGGGATTGCCTCTGACCAAAAGATCCGCAGCTTAGTGAGTGACTATGTCCTTTTAGAAAAGGAACACAAAGCGCTCAGTGAAGCTAAACCAAATGACCCTAAAGTAGGTCAGTTAGAAGCGGACATGGATGAGTTGATGGATAAAATCATCACCGAACGGGCTGCTAAACACATTGTTCATGAATTGTCCATTTCACTACGTGGTTTAGAAAACACCGATGAATGTCCGGAAAACAGTTACCACGCTTTACTGTGTTCACGTTTCTTCCGTCAAAGAAGGTATAACCTCTCCGCTGCTGGTCGTTATAAGGTATCACGTAAGTTAAGAATTACCGAGCGGATTTACCAAAAAACACTTGCTTGTGACCTTCATTTAAAAAACGGGGAACTCTTATTAAAGAAGGGCACCTTATTAGTTAAAGAAGAAATCGACAAGATTAAGCAAGCTGCCCAAAACAACCAAATTGACTTTGTCCAAAAGATTAAGTTAACCACTGATGGTAGTGCTGTTAACCTTTCCCCTGAATCGTTGTTGTATGAATCACTTGATGTTTATGTGAACAACGACAACTTCGATGTATCCGTGCCAGTGGTGGGGATCCACAACGATAACGATCTCAATAAGGCAATTACTTTAAGTGACTTTATTGCCTCGATTAGTTATGTGATTAACATCCCTTCGGCCATCGGTAAGTACGATGACATTGATCACTTAGGTAACAAACGCGTCAAGTTAATTAACGAGTTAATTAGTTCCCGTTTGGAAAGTGGAATCACCCGCATGGAGCGCTTCTTAAAGGAAAAGCTCACGATTGCTGATGGAGTGAACCGTGGTCAACAAATTAACGAAGAGGGTCAAGTAATTGAACAAGCGGAAAAGAAGGAATTGACCATTAAATCCTTAATTAATTCCAAACCTATTCAAATTGTCATTCGTGACTTCTTCAACACTCACCAGTTGACCCAGTTCTTAGATCACCAAAATCCACTTTCAGAATTAAGTAATAAGCGCCGGATTTCCGCCATGGGTCCAGGGGGTATCTCCCGCGAAGACCCGAACTTGGATATCCGGGATGTGCACTATTCCCAATATGGTCGGATTTGTCCAATTGAAACACCGGAAGGGATGAACATTGGTTTAATCATGTCGTTGGCTTCCTTTGCCAAGATTGACGAAAACGGATTCCTAATGGCGCCTTACCGCAAGATTAAAAATGGTGTCATTACCGATGAAGTTGAATACCTCACGGCATTAAGGGAAGACGAGCACATTATTGCCGAAATCTCTTCGTTGGTCAACATTGACGAAAACAACAAGATCTTAGATAAGGAAATTATTGGTCGTTACCGCTCGATGCAAGGGTTGTATGATCCCTCGAAGATTGACTACATCGACGTGGCCCCTCATCAGGTAGTGTCCATCGGTTCGTCCTTAATTCCCTTCTTGGAAAACGATGACTCAGCGCGGGCATTGATGGGTACCAACATGCAACGCCAAGCCTATCCTTTAATTAAGCCTTACGCTCCCGTTGTGGGTACAGGTCAAGAGTACAAGATTGCTCGCGACTCCGGTTTGACGATGTTAGCACCTTGCTCAGGTACGGTAAAGTATGTAGACAACAGTAAGATCACGATTGAAAGTGACAGTGGTGAACAGCACACTTTGGATCTAATTAAGTTTGAACGTTCCAACCAAAATACCTGCTACAACCACGTTCCCTTAGTAGAAAAGGGGCAACGGGTAACTAAGGACGAAGTGATTGCTGATGGTCCTGCAGTTAACAAGAGTGAGCTGTCTTTAGGGCAAAATGTCCTTGTCGCCTTTACGACCTGA", "aa": "MSQKPSFFQKKYSPTATRRYYGKIATDFVQPNLADIQIRSYQTFLDHDLENLIAAYFPIKSPNDRYTINFKGLRRTAPERNEAQSRSESKTYEIGIYADLELIDSATGTIKKPRKSKKNSATSSVDGVFLTNLPLITRDGVFIVNGIEKFVIAQITRSPGIYMLTKSQLKLSSSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGMSGKEILKVFKNNEFITRSLEAEVYNAKDFLNNVDPEIKNLLREFRDGKTDLRRKGIASDQKIRSLVSDYVLLEKEHKALSEAKPNDPKVGQLEADMDELMDKIITERAAKHIVHELSISLRGLENTDECPENSYHALLCSRFFRQRRYNLSAAGRYKVSRKLRITERIYQKTLACDLHLKNGELLLKKGTLLVKEEIDKIKQAAQNNQIDFVQKIKLTTDGSAVNLSPESLLYESLDVYVNNDNFDVSVPVVGIHNDNDLNKAITLSDFIASISYVINIPSAIGKYDDIDHLGNKRVKLINELISSRLESGITRMERFLKEKLTIADGVNRGQQINEEGQVIEQAEKKELTIKSLINSKPIQIVIRDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKNGVITDEVEYLTALREDEHIIAEISSLVNIDENNKILDKEIIGRYRSMQGLYDPSKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPVVGTGQEYKIARDSGLTMLAPCSGTVKYVDNSKITIESDSGEQHTLDLIKFERSNQNTCYNHVPLVEKGQRVTKDEVIADGPAVNKSELSLGQNVLVAFTT", "aa_hexdigest": "ba37769ed89d798322ab4f5e11afd33b", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoB" ], "truncated": "5-prime", "pseudogene": { "start": 628196, "stop": 631198, "observations": { "mutation": [ 628501 ], "stop": [ 628501 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78013", "query_cov": 1.0, "subject_cov": 0.6458333333333334, "identity": 1.0, "score": 1738.0, "evalue": 0.0, "gene_start": 1, "gene_end": 899, "reference_start": 1, "reference_end": 899, "reference_sequence": "MSQKPSFFQKKYSPTATRRYYGKIATDFVQPNLADIQIRSYQTFLDHDLENLIAAYFPIKSPNDRYTINFKGLRRTAPERNEAQSRSESKTYEIGIYADLELIDSATGTIKKPRKSKKNSATSSVDGVFLTNLPLITRDGVFIVNGIEKFVIAQITRSPGIYMLTKSQLKLSSSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGMSGKEILKVFKNNEFITRSLEAEVYNAKDFLNNVDPEIKNLLREFRDGKTDLRRKGIASDQKIRSLVSDYVLLEKEHKALSEAKPNDPKVGQLEADMDELMDKIITERAAKHIVHELSISLRGLENTDECPENSYHALLCSRFFRQRRYNLSAAGRYKVSRKLRITERIYQKTLACDLHLKNGELLLKKGTLLVKEEIDKIKQAAQNNQIDFVQKIKLTTDGSAVNLSPESLLYESLDVYVNNDNFDVSVPVVGIHNDNDLNKAITLSDFIASISYVINIPSAIGKYDDIDHLGNKRVKLINELISSRLESGITRMERFLKEKLTIADGVNRGQQINEEGQVIEQAEKKELTIKSLINSKPIQIVIRDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKNGVITDEVEYLTALREDEHIIAEISSLVNIDENNKILDKEIIGRYRSMQGLYDPSKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPVVGTGQEYKIARDSGLTMLAPCSGTVKYVDNSKITIESDSGEQHTLDLIKFERSNQNTCYNHVPLVEKGQRVTKDEVIADGPAVNKSELSLGQNVLVAFTTWNGYNYEDAIVISERLVKDDVLTSLTINEYVAQCLSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKLPHGGDGIVSCVKRFSIANGNELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPVADMPHLEDGTPVXDILLNPLGVPSRMNIGQIFEMHLGYAAHNLAKRMLISACFDDKKAQALSTEINQPQYKLDRLITGLKAQITNRGLKDEQAALAQLNNGDIALVLKEIGMSFDDLHFKVATPIFQGVNFQDLQDIMDEAGLKPAETHGKFKLIDGRTGLPFEKPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEVWALEAYGAAYNLQELLTIKSDDVQGRNKAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQADGEQDDLFNDFEFDTEGY" }, "paralog": false, "identity": 0.999, "score": 1950.0, "evalue": 0.0, "description": "Internal stop codon at 628501. Nonsense mutation around 628501.", "psc": { "uniref90_id": "UniRef90_P78013", "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47715", "cog_id": "COG0085", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78013", "EC:2.7.7.6", "UniRef:UniRef50_P47715", "COG:COG0085", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "pscc": { "uniref50_id": "UniRef50_P47715", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47715" ], "product": "DNA-directed RNA polymerase subunit beta" } }, "id": "GIHJHMOALN_1112", "locus": "GCA009939745_01108" }, { "type": "cds", "sequence": "contig_1", "start": 631291, "stop": 631791, "strand": "-", "frame": 1, "gene": "ssuE", "product": "putative MG342-like protein", "db_xrefs": [ "COG:C", "COG:COG0431", "GO:0005524", "GO:0005829", "GO:0010181", "GO:0016491", "RefSeq:WP_010874873.1", "SO:0001217", "UniParc:UPI000013965D", "UniRef:UniRef100_P75270", "UniRef:UniRef50_P75270", "UniRef:UniRef90_P75270" ], "nt": "ATGAGTACCAAACCGTTAATCTTATTGTTAGCTAACTCCAAAAATTCAATTAACCGGAAGTTTGCTAAAGCTTTGGAACAACAACTCAATGCTGAATTAATTGAACTAGTTGACTACCAAGTGGATTTTTATTGTGAAGATTTGGAAAAGGATCATTTTCCCGAAAAAATTAAGAGTTTAGTGCGCAAACTACACGATCACAAAACGCTAATTTTTGTCACTCCAGAACACAACGGTTTTGTCCCTGCCTTTGCTAAAAACACAATCGACTGGATGACCCGCGATACCCAATACGGTAAAAACCAGTTTTTAAAGGAGCTTGATGGAATTATTTGCTGTGTCACTCCTGCAGCTAAGAGTGGTGGTAAGACAGTGCTGGAACTACTCACTAAATTCTTTAGTTTTAGTGGTCTCAACGTTAAGGGAGCCGTGTTAGTAAATGGCTATCATGATGGCTTTGATTTTCAGCCATTTATCACTGACGTACAAAAATTGATATAA", "aa": "MSTKPLILLLANSKNSINRKFAKALEQQLNAELIELVDYQVDFYCEDLEKDHFPEKIKSLVRKLHDHKTLIFVTPEHNGFVPAFAKNTIDWMTRDTQYGKNQFLKELDGIICCVTPAAKSGGKTVLELLTKFFSFSGLNVKGAVLVNGYHDGFDFQPFITDVQKLI", "aa_hexdigest": "e2a5d7c7594bd7c1481c1e3a67847f94", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013965D", "ncbi_nrp_id": "WP_010874873.1", "uniref100_id": "UniRef100_P75270", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013965D", "RefSeq:WP_010874873.1", "UniRef:UniRef100_P75270" ] }, "ips": { "uniref100_id": "UniRef100_P75270", "uniref90_id": "UniRef90_P75270" }, "psc": { "uniref90_id": "UniRef90_P75270", "gene": "SsuE", "product": "Uncharacterized protein MG342 homolog", "uniref50_id": "UniRef50_P75270", "cog_id": "COG0431", "cog_category": "C", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0010181", "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75270" ], "product": "Uncharacterized protein MG342 homolog" }, "genes": [ "ssuE" ], "id": "GIHJHMOALN_1113", "locus": "GCA009939745_01109" }, { "type": "cds", "sequence": "contig_1", "start": 632221, "stop": 632439, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6P5", "UniRef:UniRef90_A0AB38W6P5" ], "nt": "GTGAAAGTTTTGGTAGTTGTGAAACTAATAAAAAAAGATGTTTATTGTTTTCAATTCTTATTTAAGAAAAATTTAGAGGAAAACTTAGAGGATGATCATAAAGAAGAGGTATTGGATAGAATAGAAGGTTTATTCAAAAGTCTAAGAAAGGGAGGAAGCTTTAAAGAAGACGATCAGTCTAAAGATTTAATTAAGCATTTAAAAAGCATTAAAAAATGA", "aa": "MKVLVVVKLIKKDVYCFQFLFKKNLEENLEDDHKEEVLDRIEGLFKSLRKGGSFKEDDQSKDLIKHLKSIKK", "aa_hexdigest": "f9b4401fcf027851af0d30774ad05cf4", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 632026, "stop": 632439, "observations": { "mutation": [ 632061, 632133, 632223 ], "stop": [ 632061, 632133, 632223 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W6P5", "query_cov": 1.0, "subject_cov": 0.5255474452554745, "identity": 1.0, "score": 140.0, "evalue": 2.36e-41, "gene_start": 1, "gene_end": 72, "reference_start": 1, "reference_end": 72, "reference_sequence": "MKVLVVVKLIKKDVYCFQFLFKKNLEENLEDDHKEEVLDRIEGLFKSLRKGGSFKEDDQSKDLIKHLKSIKKWSENEDKKHLKELKKYLKSSMDTKLPSEDHWNAKEAKERLASAKKDALLELDSEWYTTFDHDKRE" }, "paralog": false, "identity": 0.9708029197080292, "score": 260.0, "evalue": 3.19e-91, "description": "Internal stop codon at 632061, 632133, 632223. Nonsense mutation around 632061, 632133, 632223.", "psc": { "uniref90_id": "UniRef90_A0AB38W6P5", "uniref50_id": "UniRef50_A0AB38W6P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W6P5", "UniRef:UniRef50_A0AB38W6P5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6P5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8552.959799999993, "isoelectric_point": 9.015017127990724 }, "id": "GIHJHMOALN_1114", "locus": "GCA009939745_01110" }, { "type": "cds", "sequence": "contig_1", "start": 632889, "stop": 633134, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTGTATTTTGAAAACTTGCAAGTGTTTGCCCAAGAAGCTAGCACTTTACAAGTTAATGCAATTGTGATTAAACAAGATGCCTATTTTATTGTTGAAGTAATGAAGGGTTACAACTATGACAATAATTGCATCTGTTTATTAGAGGCCAATAAAGATCAAAACGTTGGTTTAACCACCGTAAGCACTAATACTCTAATAGGTGACATCAAGCAATACGAGCCTTGTTTCTTTATTGAAAAGTAA", "aa": "MLYFENLQVFAQEASTLQVNAIVIKQDAYFIVEVMKGYNYDNNCICLLEANKDQNVGLTTVSTNTLIGDIKQYEPCFFIEK", "aa_hexdigest": "b89cb279c8548b1e591f5ffe155e6a8e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9280.518199999995, "isoelectric_point": 4.31762638092041 }, "id": "GIHJHMOALN_1115", "locus": "GCA009939745_01111" }, { "type": "cds", "sequence": "contig_1", "start": 633168, "stop": 633935, "strand": "-", "frame": 2, "gene": null, "product": "putative MG343-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75269", "UniRef:UniRef90_P75269" ], "nt": "ATGGCACAAACAACCATTAACGAGCAAACCTTGGTGAATGAGCAAAGACTTTTAGCTTACGATTGCTTTCAAAACACCAATAAAGTGGGATTACTTTCCGCTTTAGAATACTTAGATTTTGTCAATCACCTAGTTAAGTCGAATCAGATTAAATACTTATTAGTACCATCAGCGAGTCAAACAGTTTACCAACACGTTTTCTTTGATCACTTTCGCTTTTTAAGTTTTGACAATAACTTATTAAGTGGTTTTGGTTTAGCGATTAGGTTACATAAAGAAGCGGAAACAGTGTTTATGTTGGTCGAAAAAACCAAACAAACAGAAACAGAAATTAAAAAGCTGTTAGAAGCAGTTAAACAGTTTTCCGGGCTGAAATTAGTGGTTTTACTAGTTAATTCTCCGTTTGAAAATAAGCGAACAAAGCTTTCTTATGAAATCGTTAAAATCATTAAAAAGCACGGTTATCAGTACAAACGTTATTGCCGCAAAAACTTTGAAAAGAAGTTTTTTAATACTTGGAACCACCAAAAGCGCAAGCAAAAAACAAAGAATAACCTTTTCTTTGTCGAGTTAAATTCACAGTTTGCTTTTGGCACTTCTATGGCTAACAGTAACAGTTTTGACCTGAGCTTTACCAGTTTCAAAGAACGCTTTAATACTGAAAATAACTTAGACATTGATGTGCACTTCAACGTACCACACAAGTATTTACTCAAAAACTTGGAGAGTATTAAGCACCACTTACAAATAGATAACAATACCATTTGA", "aa": "MAQTTINEQTLVNEQRLLAYDCFQNTNKVGLLSALEYLDFVNHLVKSNQIKYLLVPSASQTVYQHVFFDHFRFLSFDNNLLSGFGLAIRLHKEAETVFMLVEKTKQTETEIKKLLEAVKQFSGLKLVVLLVNSPFENKRTKLSYEIVKIIKKHGYQYKRYCRKNFEKKFFNTWNHQKRKQKTKNNLFFVELNSQFAFGTSMANSNSFDLSFTSFKERFNTENNLDIDVHFNVPHKYLLKNLESIKHHLQIDNNTI", "aa_hexdigest": "bf619e378be14f057abde21c21c8ccb1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 632889, "stop": 633935, "observations": { "mutation": [ 633170 ], "stop": [ 633170 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75269", "query_cov": 1.0, "subject_cov": 0.7327586206896551, "identity": 1.0, "score": 492.0, "evalue": 3.64e-174, "gene_start": 1, "gene_end": 255, "reference_start": 1, "reference_end": 255, "reference_sequence": "MAQTTINEQTLVNEQRLLAYDCFQNTNKVGLLSALEYLDFVNHLVKSNQIKYLLVPSASQTVYQHVFFDHFRFLSFDNNLLSGFGLAIRLHKEAETVFMLVEKTKQTETEIKKLLEAVKQFSGLKLVVLLVNSPFENKRTKLSYEIVKIIKKHGYQYKRYCRKNFEKKFFNTWNHQKRKQKTKNNLFFVELNSQFAFGTSMANSNSFDLSFTSFKERFNTENNLDIDVHFNVPHKYLLKNLESIKHHLQIDNNTIWKQAFINFAGQLLLYFENLQVFAQEASTLQVNAIVIKQDAYFIVEVMKGYNYDNNCICLLEANKDQNVGLTTASTNTLIGDIKQYEPCFFIEK" }, "paralog": false, "identity": 0.9942528735632183, "score": 693.0, "evalue": 8.22e-256, "description": "Internal stop codon at 633170. Nonsense mutation around 633170.", "psc": { "uniref90_id": "UniRef90_P75269", "product": "Uncharacterized protein MG343 homolog", "uniref50_id": "UniRef50_P75269", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75269", "UniRef:UniRef50_P75269" ] }, "pscc": { "uniref50_id": "UniRef50_P75269", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75269" ], "product": "Uncharacterized protein MG343 homolog" } }, "id": "GIHJHMOALN_1116", "locus": "GCA009939745_01112" }, { "type": "cds", "sequence": "contig_1", "start": 633938, "stop": 634246, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGTTAGACAAGCATACTGCTATGTGCAAAAAACTCACCTTTCAGATCTTTAAGGAATTGTCGCGCTTAAACAGTGCTTATAAAACTATTACCATACCAGCCTTTTTACTGTTGGCTCAACATGATGATTTTATGCCTACAAAAGCTACTTTAAACTACTTTAACCGCTTTTTGATTAAGAAAGGTAACTTGCAATCAGGGGTTATCTTACACAGTCAACACCAAATGTTTAACAGCAAGCATGAAAGTTTCTGTAAAGCAATGCACGATATTTTGAAGCATAATAAACTAAGTAAAATTTACTAG", "aa": "MELDKHTAMCKKLTFQIFKELSRLNSAYKTITIPAFLLLAQHDDFMPTKATLNYFNRFLIKKGNLQSGVILHSQHQMFNSKHESFCKAMHDILKHNKLSKIY", "aa_hexdigest": "5dff4fa48a3661def8ac3fa04d03498e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11971.97859999999, "isoelectric_point": 9.69825305938721 }, "id": "GIHJHMOALN_1117", "locus": "GCA009939745_01113" }, { "type": "cds", "sequence": "contig_1", "start": 634250, "stop": 634456, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGGGGCAGTGGCTAGTTATGCATTGCAGTTTTTAAAACCACAACGAGTGGAAAAACTAGTGCTCTTAGCGCCACTGTCTTATTCCAACTTGTTGAATTACTACAAGATTAAAAAGGCTTTTAAAAAGAAGGACGAAAAGCTGTCTTACTTTAAGCGGATGTTTCAACCAAAGTTTCCTGATTTACAAAACGATGGACAGTGA", "aa": "MGGAVASYALQFLKPQRVEKLVLLAPLSYSNLLNYYKIKKAFKKKDEKLSYFKRMFQPKFPDLQNDGQ", "aa_hexdigest": "5aab33eaa30b1b6da90de7cda195fd41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7946.292999999999, "isoelectric_point": 9.889272880554199 }, "id": "GIHJHMOALN_1118", "locus": "GCA009939745_01114" }, { "type": "cds", "sequence": "contig_1", "start": 634577, "stop": 634756, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAGACAAAGTCAATATTAATACTGTTGATATTAGCCAACTAGAAGTATTTTTTCAACCAGCTAAAAAACCCGCTAAACAAACTATTGTGTTTGCGCACGGTTTTTCAGTGCACCATTCTTATTTTAAGAGCTTTTCTGAAACGCTGGTGGATTATGACTATTACGCACCCTTGTGA", "aa": "MRDKVNINTVDISQLEVFFQPAKKPAKQTIVFAHGFSVHHSYFKSFSETLVDYDYYAPL", "aa_hexdigest": "f3be089e3822adf74f66f4f7186c04ae", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6869.7207, "isoelectric_point": 6.813312721252441 }, "id": "GIHJHMOALN_1119", "locus": "GCA009939745_01115" }, { "type": "cds", "sequence": "contig_1", "start": 634759, "stop": 635163, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAAAAAGCCGGTTAGTGTAAACCTGTTTGCAGGACCAGCCATGTTTAAAGTTGCTAATGTTAAAGGGTTAGATCGCTTACACGAAAGCTTTAGCGCCATTAAAGACCGGGCTTATGCAGCCATTGAACAAGCACGTCAAAATGGTGTGATTACTAAAAACAACCAGGTTGTATTGACATTGGGTGTGGATTCTACTAGTGCAATTGATCAACGTCTTGTAAAACATTTAGCAGTTTGGCTAAACGTAAACCAGGTAAATATAACGAATGACCCCAACGAAATTAAGGTTGAACACACCGATCAAGTCATGTGTGAACGATGTTGGAACTTCCAAACCCAAATTTACCCTAAAAAAGGACACCAATTGTGTGCAAGGTGTTTTACTTTTCTTAAAAGGTAA", "aa": "MPKKPVSVNLFAGPAMFKVANVKGLDRLHESFSAIKDRAYAAIEQARQNGVITKNNQVVLTLGVDSTSAIDQRLVKHLAVWLNVNQVNITNDPNEIKVEHTDQVMCERCWNFQTQIYPKKGHQLCARCFTFLKR", "aa_hexdigest": "4f70531f0f68287ee56a6c328711dd4e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15209.438499999995, "isoelectric_point": 9.474805641174317 }, "id": "GIHJHMOALN_1120", "locus": "GCA009939745_01116" }, { "type": "cds", "sequence": "contig_1", "start": 635176, "stop": 635589, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "GTGGACAACCGCATAGGTGACAACATTATCAAGCAAATTGTCGAGCAATACCGCCGCATTCGCAACAGCTTATTGCGCTTTATATTGGGTAACCTCAACCACTTTAACTTTGGTGAGATGAAGGATTATCGTTTTGCTCTAGAGGACAAAATCGTAATCCATAAAACTAATGCATTGGTACAAGAATTGCACCAGTGGTTAAAGCAATACAACTTTTTAAATTGCTTAAAGGCCATTAATAAGTTTGTGTTGTGGTTATCGGGTTGGTATTTTGAAATTATTAAGGACACACTTTATTGTGATGCTAAAACTAATCCTAATCGGTTGGCTAAGCAGGCAGTATTGAACTATATCTTTACCCAGTTAATTGGTTTCTTAAATATCTTTATTCCCCATACTGCCGAAGATGCTTGA", "aa": "MDNRIGDNIIKQIVEQYRRIRNSLLRFILGNLNHFNFGEMKDYRFALEDKIVIHKTNALVQELHQWLKQYNFLNCLKAINKFVLWLSGWYFEIIKDTLYCDAKTNPNRLAKQAVLNYIFTQLIGFLNIFIPHTAEDA", "aa_hexdigest": "98e8e502d76f64856c5f398eb523986b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 89, "aa_cov": 0.6423357664233577, "hmm_cov": 0.9550561797752809, "evalue": 9.927321881540822e-18, "score": 59.92002868652344, "start": 49, "stop": 136 } ], "seq_stats": { "molecular_weight": 16338.79909999999, "isoelectric_point": 9.120100593566896 }, "id": "GIHJHMOALN_1121", "locus": "GCA009939745_01117" }, { "type": "cds", "sequence": "contig_1", "start": 635593, "stop": 636075, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGCCGATTCCCTTAATTTATGCAGATAACCAACCCTTACTGGATGTGACTACCATTAAATACACCATTCAACAGCTCAAAAAGTACGGGATTGACAGTTGGTTTGAAAAGGATATTAATTTTTTTTTAAACCCAAAGAAAATACAGCCAGGAGTTGAATATAAAAAGGAAACTGATACCTTGGAAGTATGGTTTGACTCTGGTTCAACTTACAACGTTTTAATTAGTAATAAACTGAACTTTCCCGCTGATCTTTATTTGGAGGGTAGTGACCAATACCGCGGTTGGTTTAACTCATCCGCCAGTTGCGGTATTATCCAAACTGACCAATTACCCTTCAAGAGTTTGATTTCCCATGGTTTTACCTTGGATGAACATGGTAATAAGATGTCCAAGTCACTCGGTAATGTGGTCGACCCTTTAAAGTTGTGTGATCAGTATGGCGCAGACATTCTGCGTTTATGGGTAACTAATGTTGATTGA", "aa": "MPIPLIYADNQPLLDVTTIKYTIQQLKKYGIDSWFEKDINFFLNPKKIQPGVEYKKETDTLEVWFDSGSTYNVLISNKLNFPADLYLEGSDQYRGWFNSSASCGIIQTDQLPFKSLISHGFTLDEHGNKMSKSLGNVVDPLKLCDQYGADILRLWVTNVD", "aa_hexdigest": "cdce0c59656cff9ae2a743dd0e82c19e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 167, "aa_cov": 0.98125, "hmm_cov": 0.9760479041916168, "evalue": 1.0736662587834312e-39, "score": 131.8479461669922, "start": 2, "stop": 158 } ], "seq_stats": { "molecular_weight": 18293.550699999996, "isoelectric_point": 4.967182350158691 }, "id": "GIHJHMOALN_1122", "locus": "GCA009939745_01118" }, { "type": "cds", "sequence": "contig_1", "start": 636079, "stop": 636246, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGATTTACCGTGCCTCCAAACAGCTCTTCATTAAAACTAAATCAATTCAAAGTAAGCTAAAGCGTCAAATTAAAAGGGTTAAGTTTGTTAATAACAAGAATAAAGAGCGCCTACAAGAAATGCTGTTGCAACGTGCTGAATGGTGTATTTCCAGACAACGAGTATGA", "aa": "MIYRASKQLFIKTKSIQSKLKRQIKRVKFVNNKNKERLQEMLLQRAEWCISRQRV", "aa_hexdigest": "252389a40024f64179a19fcfbe583db8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 54, "aa_cov": 0.9818181818181818, "hmm_cov": 1.0, "evalue": 4.3325171145410316e-12, "score": 40.91628646850586, "start": 2, "stop": 55 } ], "seq_stats": { "molecular_weight": 6793.0944, "isoelectric_point": 11.394609642028808 }, "id": "GIHJHMOALN_1123", "locus": "GCA009939745_01119" }, { "type": "cds", "sequence": "contig_1", "start": 636262, "stop": 636549, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACCCGGTGTTGTTGGGTAATCACGTTTTATGTAATGAGGGTACAGGACTCGTGCATACTTCACCAGCTTATGGTTTGGATGACTTTTATTTGTGCAAGCAAAACAAGTTAAATGAAGCGCTAGTTTCACTTGATGAAAAAGGAGTTTTTAATGATACGTTGAACGATCCAGTTTTAACGGGTTTGTTTTATCTAAAGGCTAATGATGTGATTATTGAACGCTTAAAGCAACACCATAACTTTGTCTTTAGTGAAAGCTTTTTGCACCGCGAACCCCACGATTGA", "aa": "MNPVLLGNHVLCNEGTGLVHTSPAYGLDDFYLCKQNKLNEALVSLDEKGVFNDTLNDPVLTGLFYLKANDVIIERLKQHHNFVFSESFLHREPHD", "aa_hexdigest": "fedd404a89f9918e5212c7f1fb1d71e8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10781.060099999992, "isoelectric_point": 5.345559501647949 }, "id": "GIHJHMOALN_1124", "locus": "GCA009939745_01120" }, { "type": "cds", "sequence": "contig_1", "start": 636901, "stop": 637194, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "GTGTTTATTCCTGGTTGGGATTGCCATGGTTTGCCCATAGAGCATGCGGTGAGCAAAAAAGATCCGCAACACTATGCGAGCTTATCATTGAGTGAAAAGCGCGATTTATGCAAGCAATTTGCCCTGTCACAAATTGCCATTCAAAAAGCCCAGTTTCAACGCTTAGGTCTGTTGAATGACTTTAGTAAGTATTACAAAACAATTGATGAAAGCTTTCAACAAAATGAGCTAGACCTGTTTTTACAAGCAGTGAAAAAGGATTTAATCTTCCAAGCGTTAAAACCAACTTACTGA", "aa": "MFIPGWDCHGLPIEHAVSKKDPQHYASLSLSEKRDLCKQFALSQIAIQKAQFQRLGLLNDFSKYYKTIDESFQQNELDLFLQAVKKDLIFQALKPTY", "aa_hexdigest": "6ba38bf4d506117874a4f2508a75eaf1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 98, "aa_cov": 0.9690721649484536, "hmm_cov": 1.0, "evalue": 5.087204286903894e-21, "score": 70.33189392089844, "start": 2, "stop": 95 } ], "seq_stats": { "molecular_weight": 11278.85899999999, "isoelectric_point": 7.773664283752441 }, "id": "GIHJHMOALN_1125", "locus": "GCA009939745_01121" }, { "type": "cds", "sequence": "contig_1", "start": 637366, "stop": 637458, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTAAAAAAGACACTGTTAATGCCCCAAACTGCATTTGAAATGCAGGGCAAATTAACCACTAAGGAACAGCAGTTTCAAGCTTTTTGA", "aa": "MNLKKTLLMPQTAFEMQGKLTTKEQQFQAF", "aa_hexdigest": "f6888f38c3847dcbb77891cbd9482cf2", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3532.1588, "isoelectric_point": 9.524639701843263 }, "id": "GIHJHMOALN_1126", "locus": "GCA009939745_01122" }, { "type": "cds", "sequence": "contig_1", "start": 637501, "stop": 637899, "strand": "+", "frame": 1, "gene": "trmL", "product": "tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL", "db_xrefs": [ "COG:COG0219", "COG:J", "EC:2.1.1.207", "GO:0002130", "GO:0003723", "GO:0005737", "GO:0006400", "GO:0008175", "GO:0141098", "GO:0141102", "KEGG:K03216", "SO:0001217", "UniRef:UniRef50_P47588", "UniRef:UniRef90_P75257" ], "nt": "ATGTATAAATCAGCGATTAATATCGTCTTATTTTGCCCAGAAATTCCTAATAACACTGGGAACATTGTTCGGAGCTGTACCGCTTTTAAAGCTAACTTGCATCTGATTAAGCCCTATGGCTTTTTTCTCAATGATAAGCGGATGGTGCGTGCTGGGTTAAATTGTTGGGATAAGGTGCAAATGTTTGAACACAAATCCTGGGAACACTTCATTGAAACAACACCACCAAATAAAGCAATTTGGTTGTTGACAAAGAGTGGTACTACTACCCCAGACCAAATTAACATGCAAACAGATAACAGGGAACAACTGTACTTTGTTTTTGGTCAAGAAACCAAGGGATTACCGCAAACACTCATGGAACAATACTCGCAGAACCAGGTACGGATTCCAATTTGA", "aa": "MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQMFEHKSWEHFIETTPPNKAIWLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLMEQYSQNQVRIPI", "aa_hexdigest": "f6f3fa676df99b31653d2ad76cc1e762", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmL" ], "truncated": "3-prime", "pseudogene": { "start": 637501, "stop": 638001, "observations": { "mutation": [ 637897 ], "stop": [ 637897 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75257", "query_cov": 1.0, "subject_cov": 0.7951807228915663, "identity": 1.0, "score": 283.0, "evalue": 1.49e-96, "gene_start": 1, "gene_end": 132, "reference_start": 1, "reference_end": 132, "reference_sequence": "MYKSAINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNCWDKVQMFEHKSWEHFIETTPPNKAIWLLTKSGTTTPDQINMQTDNREQLYFVFGQETKGLPQTLMEQYSQNQVRIPIWNPVRSINLSNTVACVLYEYAKQNHYFNLDKQCA" }, "paralog": false, "identity": 0.9939759036144579, "score": 347.0, "evalue": 3.38e-124, "description": "Internal stop codon at 637897. Nonsense mutation around 637897.", "psc": { "uniref90_id": "UniRef90_P75257", "gene": "trmL", "product": "tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase TrmL", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_P47588", "cog_id": "COG0219", "cog_category": "J", "kegg_orthology_id": "K03216", "go_ids": [ "GO:0002130", "GO:0003723", "GO:0005737", "GO:0006400", "GO:0008175", "GO:0141098", "GO:0141102" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75257", "EC:2.1.1.207", "UniRef:UniRef50_P47588", "COG:COG0219", "COG:J", "KEGG:K03216", "GO:0002130", "GO:0003723", "GO:0005737", "GO:0006400", "GO:0008175", "GO:0141098", "GO:0141102" ] }, "pscc": { "uniref50_id": "UniRef50_P47588", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47588" ], "product": "Putative tRNA (cytidine(34)-2'-O)-methyltransferase" } }, "id": "GIHJHMOALN_1127", "locus": "GCA009939745_01123" }, { "type": "cds", "sequence": "contig_1", "start": 637992, "stop": 638333, "strand": "+", "frame": 3, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_P47589", "UniRef:UniRef90_P75256" ], "nt": "GTGCGCTTAAGAAAAGTTAAAGATGCTCTTGTAAGGGTACAAGACAGCCCTTACTTTAGTAACCCAGAACAACTGTCAACTATTGAAAGTGAATCGCTCTTCGTGGAAATAGGTTGTGGTAAAAGTTGGTTTTTAAGCCAGCAAGCACAGCAAAACCCAAAGTGTTTTTTTGTGGGAATCGAGCGTGAACCCACGATTGTTTTAAAGGCAATTAATAAGGTAAACCGCTTAGAGGTTAAACCACGTAATTTATTAATAACGTGTTTGGATGCCAACCAGCTAACAGAGTACTTAAAACCACAATCGATTAGCAAAATCTTCATTAACTTCCCCGATCCTTGA", "aa": "MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDP", "aa_hexdigest": "51c22b221694814d10f5d606558cc1e1", "start_type": "GTG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 637992, "stop": 638624, "observations": { "mutation": [ 638331 ], "stop": [ 638331 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75256", "query_cov": 1.0, "subject_cov": 0.5380952380952381, "identity": 1.0, "score": 229.0, "evalue": 4.51e-75, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDPWPKKRHTLRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN" }, "paralog": false, "identity": 0.9857142857142858, "score": 412.0, "evalue": 2.15e-149, "description": "Internal stop codon at 638331. Nonsense mutation around 638331.", "psc": { "uniref90_id": "UniRef90_P75256", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_P47589", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75256", "EC:2.1.1.33", "UniRef:UniRef50_P47589", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_P47589", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47589" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "GIHJHMOALN_1128", "locus": "GCA009939745_01124" }, { "type": "cds", "sequence": "contig_1", "start": 638352, "stop": 638624, "strand": "+", "frame": 3, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_P47589", "UniRef:UniRef90_P75256" ], "nt": "TTGAGGCGACTAACTGCTCCACATTTCCTCAAGCAGTTTCACCAACTTTTAAAAAAGCAAGGTTTAATAGAATTCAAAACGGACAACGATCAGTTGTTTGAATTTACCTTAGAAGTCTTGCAGAAAACAGTAGCTCACTTTAAGATAATTGAGCAAACGACAGATCTGCACAATTCACCTTTGGCTAGTACCAATATTATGACCGAATACGAACAGCGCTTTGTTAGTTTAGGGGTTAAGATTAAAAAGCTAACGCTTGAAAAACTAAATTAG", "aa": "MRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPLASTNIMTEYEQRFVSLGVKIKKLTLEKLN", "aa_hexdigest": "04d7ec92c2dda8675b7d836dbeb7c203", "start_type": "TTG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "5-prime", "pseudogene": { "start": 638052, "stop": 638624, "observations": { "mutation": [ 638331 ], "stop": [ 638331 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75256", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 0.978, "score": 176.0, "evalue": 2.37e-54, "gene_start": 1, "gene_end": 90, "reference_start": 121, "reference_end": 210, "reference_sequence": "MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVKPRNLLITCLDANQLTEYLKPQSISKIFINFPDPWPKKRHTLRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVLQKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN" }, "paralog": false, "identity": 0.9894736842105263, "score": 375.0, "evalue": 2.59e-135, "description": "Internal stop codon at 638331. Nonsense mutation around 638331.", "psc": { "uniref90_id": "UniRef90_P75256", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_P47589", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75256", "EC:2.1.1.33", "UniRef:UniRef50_P47589", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_P47589", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47589" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "GIHJHMOALN_1129", "locus": "GCA009939745_01125" }, { "type": "tRNA", "sequence": "contig_1", "start": 638654, "stop": 638729, "strand": "+", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 71.9, "nt": "GCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 638660, 638662 ], "id": "GIHJHMOALN_15", "locus": "GCA009939745_01126" }, { "type": "cds", "sequence": "contig_1", "start": 638779, "stop": 639144, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTTTTATTCAAACAGTTTACAGCGGTAGTGTCAATAATCTTATTAACCAGTCCAATGTTCATGCCCAAACGGTTGGCACTGATGTAACTAAAGCAATTGTGATTGCTTTTCGCAAAAACAATTTAGATCAAATTAGTGCGCATTTCTATAATCCTGATAACAGTAACGGCAGTAATGCACCAGGTTCAAACCAACCAAATCAGGACAGTGGAAATAACGGTTCAACAACTCCAGCAGCTTCTGCTGCAGCCGCCGCTAAAAAATCCTATTCAGCTGGATCATTTGGCGTTAAACGCCATGCCGTGCAGGTAAGCATTAACTTTCTCAATTTCTTGGATAGTGTTTACACACCCTTAAACTAG", "aa": "MTFIQTVYSGSVNNLINQSNVHAQTVGTDVTKAIVIAFRKNNLDQISAHFYNPDNSNGSNAPGSNQPNQDSGNNGSTTPAASAAAAAKKSYSAGSFGVKRHAVQVSINFLNFLDSVYTPLN", "aa_hexdigest": "3362dfeb7399439e13907057fb6f45be", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12749.794599999983, "isoelectric_point": 9.1006311416626 }, "id": "GIHJHMOALN_1130", "locus": "GCA009939745_01127" }, { "type": "cds", "sequence": "contig_1", "start": 639376, "stop": 639696, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAGTGGTTTAGTTCAATTTCTAAAAAGAAAGTATCTTTTTCAACCTTACTCTTGTTAGGTTCAGGAATTGTTTTGAGTTCCTGTAGCAATATTGATAAACCAAATGTTTTTCGCACTCTTTCACAGCAAAGCGTAGAAAACAAAGTTGATTACAGTAAGCTACCTAAAGAAAATAAAACGGTTAGAAACTTAGTTTTTGGAACAGCGGAATACAATGATGGTAACTACGTTCTTGTAGTAACCACTGAAACTGACAGTTCTCAAATTAACTTTCTAAATGGTTCAAACAATCAAGCTGTTAGTACCGAAAATTGA", "aa": "MKKWFSSISKKKVSFSTLLLLGSGIVLSSCSNIDKPNVFRTLSQQSVENKVDYSKLPKENKTVRNLVFGTAEYNDGNYVLVVTTETDSSQINFLNGSNNQAVSTEN", "aa_hexdigest": "f436f68e8794e7874d1fb98a8b981eb7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11744.072799999993, "isoelectric_point": 9.09850368499756 }, "id": "GIHJHMOALN_1131", "locus": "GCA009939745_01128" }, { "type": "tRNA", "sequence": "contig_1", "start": 639711, "stop": 639796, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(gag)", "amino_acid": "Leu", "anti_codon": "gag", "score": 65.6, "nt": "GTCGGAGTGGTGGAATGGTAGACACGCAAGCTTGAGGGGCTTGTGGTCGCAAGACTGTGCCAGTTCAAGTCTGGTCTCCGACACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 639790, 639792 ], "id": "GIHJHMOALN_18", "locus": "GCA009939745_01129" }, { "type": "cds", "sequence": "contig_1", "start": 640286, "stop": 640624, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01519.22" ], "nt": "GTGAAAGTTAAATACGTTACCCAAGAACAATTTCAGGAATATAAAGATTCTAACAATCAGCGACTCATAAAGATTGAAACCACTTTGGCGGCCCAAGGTGAGCAAATAAGAATCCAGGGTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGCGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGCTTGGACAATATGGAAGTAAAAATGGACAAGATGGAAGGAAAAATAGACAAAATAGAAAATAGAATGGACAAGATGGAAGTAAAAATGGACAAAATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAA", "aa": "MKVKYVTQEQFQEYKDSNNQRLIKIETTLAAQGEQIRIQGEQIKELQIEQKAQGETLKLILQTLQKMSDRLDNMEVKMDKMEGKIDKIENRMDKMEVKMDKMEKRIDKLESK", "aa_hexdigest": "c1066b8d5a31c1c84f898ab18541d637", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01519.22", "name": "DUF16", "length": 88, "aa_cov": 0.6607142857142857, "hmm_cov": 1.0, "evalue": 7.668701284264513e-27, "score": 89.36125183105469, "start": 2, "stop": 75 } ], "seq_stats": { "molecular_weight": 13365.423299999991, "isoelectric_point": 7.832409095764161 }, "id": "GIHJHMOALN_1132", "locus": "GCA009939745_01130" }, { "type": "cds", "sequence": "contig_1", "start": 640661, "stop": 640771, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAAAAATTTCCGAAAAGGAATACAAAGCCTTAATCCGTAAAATTGGAAAAGAACACTTTGATGGTGAAAAAGAAGAGTACGGTGATGGCACAGTTGGAGTTTGA", "aa": "MKEKISEKEYKALIRKIGKEHFDGEKEEYGDGTVGV", "aa_hexdigest": "7ebb4537de1704cf08275a6cca7d1498", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4142.643400000003, "isoelectric_point": 5.72024211883545 }, "id": "GIHJHMOALN_1133", "locus": "GCA009939745_01131" }, { "type": "cds", "sequence": "contig_1", "start": 641364, "stop": 641480, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGCCGAACTATTACCGTGTCATCAAGAAAGCAACCTCATTTTCAGGGTTAGATATGATCTCTCATGCCTATGGTCAGATTGCTGGCGTTGAAGTTGTGGGTTTTTATTTATGA", "aa": "MQPNYYRVIKKATSFSGLDMISHAYGQIAGVEVVGFYL", "aa_hexdigest": "b5707dbd8c72970a7499dee8c5e630f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4254.8816, "isoelectric_point": 8.119486808776855 }, "id": "GIHJHMOALN_1134", "locus": "GCA009939745_01132" }, { "type": "cds", "sequence": "contig_1", "start": 641748, "stop": 642350, "strand": "+", "frame": 3, "gene": "dnaB2", "product": "putative MG349-like protein", "db_xrefs": [ "COG:COG3611", "COG:L", "KEGG:K03346", "SO:0001217", "UniRef:UniRef50_P75253", "UniRef:UniRef90_P75253" ], "nt": "ATGGGAAAACTGGAATATGACCGCAGCTGCCTTTCCTTTGAACAAATCGATAACATTCAGTTTGACAACGCACTGGAAATTACCGCTAACTTTGAAGCTAACTTTACCAGTAAGCAAGATTGTCTTAGCTTTAGTTTTGACTTTGAAGCTTTTCATAAACAATTAGTTAAACAAAATCTCTTTGTTAGTTTTAACCAGAAAACTAAGGCTGTTATTAATGGTTACTTTGAAAAATACCAAATAACACTAGAAGGCATATTGGAATGTGTTATCCCTAGTGTAGTTAATGATGAAGTTGACTTGGAACTGCTACAAAAATTACTAGAGCAGCTAGTTAAAAACAACACAGCTCCAATTGTTGATACTGTAATAAATGACCGCAACTTCTTTTATGATAATCAGAATTTAGCCACAGAAACAAAAGATGCAATTTCCCGTTGTCATTTGGAATACAACGCGGAAAAATACCTATTTCTCTTGTACGGTAAAGTAGAAAACGAGCAGTTAGATCTAATTCGCCGCTTAAGACAAGAGTTTGGAATAGCTGATAAGGTAATTAATCTAATAGTAGACTTCTCCTTTTGGAAAAACAACAGCATGTGA", "aa": "MGKLEYDRSCLSFEQIDNIQFDNALEITANFEANFTSKQDCLSFSFDFEAFHKQLVKQNLFVSFNQKTKAVINGYFEKYQITLEGILECVIPSVVNDEVDLELLQKLLEQLVKNNTAPIVDTVINDRNFFYDNQNLATETKDAISRCHLEYNAEKYLFLLYGKVENEQLDLIRRLRQEFGIADKVINLIVDFSFWKNNSM", "aa_hexdigest": "34fcba0d2bdc684b6bb5728a9e71afc9", "start_type": "ATG", "rbs_motif": "AAAA", "genes": [ "dnaB2" ], "truncated": "both", "pseudogene": { "start": 641448, "stop": 642605, "observations": { "mutation": [ 641478, 641592, 641676, 641697, 642348 ], "stop": [ 641478, 641592, 641676, 641697, 642348 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75253", "query_cov": 1.0, "subject_cov": 0.48426150121065376, "identity": 0.995, "score": 396.0, "evalue": 2e-136, "gene_start": 1, "gene_end": 200, "reference_start": 129, "reference_end": 328, "reference_sequence": "MQPNYYRVIKKATSFSGLDMISHAYGQIAGVEVVGFYLWLITEANAQAFNSEIRTPISRLQNAFNTTGTKNVNVPDWIYKTIGKLESLGLVRTFFSQEKSEMTFWIVEPLGWKEFNQKKHFKEKLIESMGKLEYDRNCLSFEQIDNIQFDNALEITANFEANFTSKQDCLSFSFDFEAFHKQLVKQNLFVSFNQKTKAVINGYFEKYQITLEGILECVIPSVVNDEVDLELLQKLLEQLVKNNTAPIVDTVINDRNFFYDNQNLATETKDAISRCHLEYNAEKYLFLLYGKVENEQLDLIRRLRQEFGIADKVINLIVDFSFWKNNSMWREQYILKIAESVERYRSHNNYQATLDNFIRASSLAKKQRTKTKIEKSEPETSAAPVNETDDVNYFLNRIKAINKKNRENGKHKR" }, "paralog": false, "identity": 0.9844155844155844, "score": 743.0, "evalue": 2.38e-275, "description": "Internal stop codon at 641478, 641592, 641676, 641697, 642348. Nonsense mutation around 641478, 641592, 641676, 641697, 642348.", "psc": { "uniref90_id": "UniRef90_P75253", "gene": "DnaB2", "product": "Uncharacterized protein MG349 homolog", "uniref50_id": "UniRef50_P75253", "cog_id": "COG3611", "cog_category": "L", "kegg_orthology_id": "K03346", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75253", "UniRef:UniRef50_P75253", "COG:COG3611", "COG:L", "KEGG:K03346" ] }, "pscc": { "uniref50_id": "UniRef50_P75253", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75253" ], "product": "Uncharacterized protein MG349 homolog" } }, "id": "GIHJHMOALN_1135", "locus": "GCA009939745_01133" }, { "type": "cds", "sequence": "contig_1", "start": 642586, "stop": 642888, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACACAAACGCTAAAACTCAACCAGCAATAAATGAGTTAGATAAGACTACAGTTCAAGACACAATTAAGAATTACGAACAACAACTTCAAAAAGAGCTTAATGAAGAACAACTAAAAGCTTTTAAAAAGCGCTTTAAGTATTTGAAGCTAACTAAAAGAGAAGTGATTGAAAAGGAAAGCAGGGAACTTTTATTGCAAGCATTTAGAGACTTTGAAAATTGTGAAAAGATAAAAGGCAATCATTGTCCTAATAAAGGCAATGCTCACCTCAACATCTTTCGCGAGCCACCTTTTTGA", "aa": "MENTNAKTQPAINELDKTTVQDTIKNYEQQLQKELNEEQLKAFKKRFKYLKLTKREVIEKESRELLLQAFRDFENCEKIKGNHCPNKGNAHLNIFREPPF", "aa_hexdigest": "e74725482ce126c15413da07ea46fdf6", "start_type": "ATG", "rbs_motif": "AACAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11923.459799999984, "isoelectric_point": 8.885951423645022 }, "id": "GIHJHMOALN_1136", "locus": "GCA009939745_01134" }, { "type": "tRNA", "sequence": "contig_1", "start": 643632, "stop": 643713, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 59.5, "nt": "CCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 643665, 643667 ], "id": "GIHJHMOALN_16", "locus": "GCA009939745_01135" }, { "type": "cds", "sequence": "contig_1", "start": 643851, "stop": 644195, "strand": "-", "frame": 2, "gene": null, "product": "MPN527 family putative ECF transporter permease subunit", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q9ZB72", "UniRef:UniRef90_P75251" ], "nt": "GTGAGTTTTACACGGTTGTACTTTCACATTTTTGCTCAAATTAAACTTCGTTGGTTATGGGTATTTAGTTTGGCTACTCTATCTAACACTTTGCTGTTAACAACGCTCAACGGTTTGTTAATTACCCCTTTATACTGGTATTGGTTTGGCTATGTGCCGACTGCTAACTTTGTCGAAGTGGCCAAGATATATAACAAAACACCATATTTTCACTTCTTTTTATTTGGCGTACCTAATTATTGGGTTGGCATCTTTGCTTTATACTCACTCTTTAATGTGATTAAATTCACACTAGTGAGTTTAATTGGTGTTCCAGTGATGCGTGCTTTCCAAAAGTTTTACTGA", "aa": "MSFTRLYFHIFAQIKLRWLWVFSLATLSNTLLLTTLNGLLITPLYWYWFGYVPTANFVEVAKIYNKTPYFHFFLFGVPNYWVGIFALYSLFNVIKFTLVSLIGVPVMRAFQKFY", "aa_hexdigest": "f71017177fd79b10087fa79c60faa8cb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 643827, "stop": 644495, "observations": { "mutation": [ 643853, 644297 ], "stop": [ 643853, 644297 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75251", "query_cov": 0.9912280701754386, "subject_cov": 0.5022222222222222, "identity": 0.991, "score": 226.0, "evalue": 1.26e-73, "gene_start": 2, "gene_end": 114, "reference_start": 105, "reference_end": 217, "reference_sequence": "MNGARIAFWPKKEQHQLFNLSFSAMMLALALIASFVSHFISIPFLSALKLTIDISSVFLIACAFFVSYSWALVITVALSLCSFIWDGNNWIGILTLTIANFAIVSFTRLYFHIFAQIKLRWLWVFSLATLSNTLLLTTLNGLLITPLYWYWFGYVPTANFVEVAKIYNKTPYFHFFLFGVPNYWGGIFALYSLFNVIKFTLVSLIGVPVMRAFQKFYWKKAQIVY" }, "paralog": false, "identity": 0.9864864864864865, "score": 439.0, "evalue": 1.2e-159, "description": "Internal stop codon at 643853, 644297. Nonsense mutation around 643853, 644297.", "psc": { "uniref90_id": "UniRef90_P75251", "product": "MPN527 family putative ECF transporter permease subunit", "uniref50_id": "UniRef50_Q9ZB72", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75251", "UniRef:UniRef50_Q9ZB72", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB72" ], "product": "Uncharacterized protein MG350.1" } }, "id": "GIHJHMOALN_1137", "locus": "GCA009939745_01136" }, { "type": "cds", "sequence": "contig_1", "start": 644295, "stop": 644504, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTGCAAGAATAGCTTTTTGGCCAAAAAAAGAACAACACCAACTGTTTAACCTTAGTTTTAGTGCCATGATGCTAGCGCTTGCACTAATTGCTTCGTTTGTTAGTCACTTTATCAGTATTCCGTTTTTGTCAGCACTTAAATTAACGATTGACATTTCATCAGTCTTTTTAATTGCTTGTGCTTTTTTTGTTTCTTATTCTTGA", "aa": "MNGARIAFWPKKEQHQLFNLSFSAMMLALALIASFVSHFISIPFLSALKLTIDISSVFLIACAFFVSYS", "aa_hexdigest": "550e5d77e2c6ceb6ad32534136d2f50c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7703.113999999996, "isoelectric_point": 9.1023717880249 }, "id": "GIHJHMOALN_1138", "locus": "GCA009939745_01137" }, { "type": "cds", "sequence": "contig_1", "start": 644559, "stop": 645113, "strand": "+", "frame": 3, "gene": "ppa", "product": "Inorganic pyrophosphatase", "db_xrefs": [ "COG:C", "COG:COG0221", "EC:3.6.1.1", "GO:0000287", "GO:0004427", "GO:0005737", "GO:0006796", "RefSeq:WP_014325585.1", "SO:0001217", "UniParc:UPI0001DD6421", "UniRef:UniRef100_A0AAV5N8E4", "UniRef:UniRef50_P47593", "UniRef:UniRef90_P75250" ], "nt": "ATGGACAAATTTTTAATCGATGTTACTGTAGAAATTCCTAAGAGCAGTAAGATCAAGTATGAATACGACCGCAAGACTAGTCAAATCCGTGTCGACAGGATTCTCTTTGGTAGTGAATCTTATCCACAAAACTATGGTTTTATCGCTAACACCTTAGACTGGGATGGTGATGAATTGGACTGTTTTATCTTCGCTGACCAAGCCTTTCTACCAGGTGTAGTAGTACCTACTAGAATTGTAGGAGCTTTAGAAATGGTAGATGACGGTGAATTGGATACCAAACTTTTAGGGGTCATTGATTGTGATCCGCGTTACAAAGAGATTAACTCCGTCAATGATTTACCAAAACACCGCGTTGATGAAATTATTGGTTTTTTCAAAACTTACAAATTACTACAAAAGAAAGAAGTAATTATTAAGGGAGTGCAAAGTTTGGAGTGGGCTAAGAAAGAATACCAAGTGTGTGTTGATTTAATGAAGCAATATGGTAAGTTGCCTAAGGATGAGTTTATCGCTAAAATGCAAAAACTCCATCCTGAACACTACCAAAAATAG", "aa": "MDKFLIDVTVEIPKSSKIKYEYDRKTSQIRVDRILFGSESYPQNYGFIANTLDWDGDELDCFIFADQAFLPGVVVPTRIVGALEMVDDGELDTKLLGVIDCDPRYKEINSVNDLPKHRVDEIIGFFKTYKLLQKKEVIIKGVQSLEWAKKEYQVCVDLMKQYGKLPKDEFIAKMQKLHPEHYQK", "aa_hexdigest": "ce32441bc6e16493b50aa434ceca1d07", "start_type": "ATG", "rbs_motif": "AATAA", "ups": { "uniparc_id": "UPI0001DD6421", "ncbi_nrp_id": "WP_014325585.1", "uniref100_id": "UniRef100_A0AAV5N8E4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6421", "RefSeq:WP_014325585.1", "UniRef:UniRef100_A0AAV5N8E4" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N8E4", "uniref90_id": "UniRef90_P75250" }, "psc": { "uniref90_id": "UniRef90_P75250", "gene": "ppa", "product": "Inorganic pyrophosphatase", "ec_ids": [ "3.6.1.1" ], "uniref50_id": "UniRef50_P47593", "cog_id": "COG0221", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0004427", "GO:0000287", "GO:0006796" ] }, "pscc": { "uniref50_id": "UniRef50_P47593", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47593" ], "product": "Inorganic pyrophosphatase" }, "genes": [ "ppa" ], "id": "GIHJHMOALN_1139", "locus": "GCA009939745_01138" }, { "type": "cds", "sequence": "contig_1", "start": 645257, "stop": 645640, "strand": "+", "frame": 2, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "EC:3.1.21.10", "GO:0006281", "GO:0006310", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_Q49422", "UniRef:UniRef90_E2I9M6" ], "nt": "GTGCAAGGAGCATTAACAGCGCAAAAGAAATCGAAAACTGACTATTACGGTGTTTATAAAGGCGAAATTTACTTTGACTTTGAAACTAAACAAACTAATAAAAGTAATTTTGCTCTAGCGCAGTTAGCAGAACACCAACTCAAACACCTTAAAAGAATTAATGAGATTGGTGGTATTAGCTTTTTGTTAATCTATTTTCAGGTACAAGACCTCTTATATGCGTTGCATACCAAGGATCTCTTGCAGATGATCGAAGCGCAAAAGGGTAAAAGTAAAACAATTAAGCGTGACCTTATTGATCAAAAAGCACAAAAGATAAATCTAATCTACCCAGGGGTTATAGATCTAATTGATGTAATAGAAAACTTTATAAAAGACTCTTAG", "aa": "MQGALTAQKKSKTDYYGVYKGEIYFDFETKQTNKSNFALAQLAEHQLKHLKRINEIGGISFLLIYFQVQDLLYALHTKDLLQMIEAQKGKSKTIKRDLIDQKAQKINLIYPGVIDLIDVIENFIKDS", "aa_hexdigest": "f7300cb1a6b5d99f6a2526c9b2b1d623", "start_type": "GTG", "rbs_motif": null, "genes": [ "recU" ], "truncated": "5-prime", "pseudogene": { "start": 645122, "stop": 645640, "observations": { "mutation": [ 645203 ], "stop": [ 645203 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_E2I9M6", "query_cov": 1.0, "subject_cov": 0.7383720930232558, "identity": 0.9840000000000001, "score": 244.0, "evalue": 2.61e-81, "gene_start": 1, "gene_end": 127, "reference_start": 46, "reference_end": 172, "reference_sequence": "MISANRGMFLETIVNQTIVRLGDHPDIWLEKRYLPIQPTAFKETRVQGALTAQKKSKTDYYGVYKGEIYFDFETKQTNKSNFALAQLAEHQLKHLKRINEIGGISFLLIYFQVQDLLYALDTKDLLQMIEAQKGKSKTIKRDLIDQKAQKINLIYPGVIDLIDVIENFIKDS" }, "paralog": false, "identity": 0.9883720930232558, "score": 332.0, "evalue": 2.96e-118, "description": "Internal stop codon at 645203. Nonsense mutation around 645203.", "psc": { "uniref90_id": "UniRef90_E2I9M6", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_Q49422", "kegg_orthology_id": "K03700", "go_ids": [ "GO:0006281", "GO:0006310" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_E2I9M6", "EC:3.1.21.10", "UniRef:UniRef50_Q49422", "KEGG:K03700", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_Q49422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49422" ], "product": "Holliday junction resolvase RecU" } }, "id": "GIHJHMOALN_1140", "locus": "GCA009939745_01139" }, { "type": "cds", "sequence": "contig_1", "start": 645652, "stop": 645966, "strand": "-", "frame": 1, "gene": "hupA", "product": "putative MG353-like protein", "db_xrefs": [ "COG:B", "COG:COG0776", "GO:0003677", "GO:0030527", "SO:0001217", "UniRef:UniRef50_P47595", "UniRef:UniRef90_P47595" ], "nt": "ATGGAAAAAACAACAACATCGAGTAAGCCATTGTCTCGTAGCGAAATTAACAAGATTATTGCAGTAGCTACTGGAGTAAAAGAGGCTAAGATAAAAGAGATCTTTAAATACCTCAACACCCTCTTACTTAATGAATTAGTAAGTCGTAGCGTTTGCATTTTGCCCGAAAATTTGGGTAAATTACGCATTACGATTCGTAACGCTAGAATTCAAAAAGACATGAAAACTGGTGAAATGAAACACATTCCACCAAAACCATTAGTGCGTTACAGTCCAAGCAAAACCATTAAGGAAACGGCTGCTAAGGTACGCTGA", "aa": "MEKTTTSSKPLSRSEINKIIAVATGVKEAKIKEIFKYLNTLLLNELVSRSVCILPENLGKLRITIRNARIQKDMKTGEMKHIPPKPLVRYSPSKTIKETAAKVR", "aa_hexdigest": "3b0cdc9dd4db5fa729fc2ead1a810834", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47595", "query_cov": 1.0, "subject_cov": 0.9541284403669725, "identity": 0.9129999999999999, "score": 178.0, "evalue": 2.14e-56, "valid": true, "gene": "HupA", "product": "Uncharacterized protein MG353 homolog", "uniref50_id": "UniRef50_P47595", "cog_id": "COG0776", "cog_category": "B", "go_ids": [ "GO:0003677", "GO:0030527" ] }, "pscc": { "uniref50_id": "UniRef50_P47595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47595" ], "product": "Uncharacterized protein MG353" }, "genes": [ "hupA" ], "id": "GIHJHMOALN_1141", "locus": "GCA009939745_01140" }, { "type": "cds", "sequence": "contig_1", "start": 646115, "stop": 646525, "strand": "+", "frame": 2, "gene": null, "product": "putative MG354-like protein", "db_xrefs": [ "RefSeq:WP_010874887.1", "SO:0001217", "UniParc:UPI0000139690", "UniRef:UniRef100_P75248", "UniRef:UniRef50_P47596", "UniRef:UniRef90_P75248" ], "nt": "ATGGAACCAAATAATTTAAAAGAAGAGCTAGTTTCAGTCTTTGAAAAAGCTTGTTCTTCACATAAAGAAAGGCTTGATTTTATCTGTTCTGTAAGAGAAAGCGATACCTTTTCCAATGTGGATGTACCGCTAGCTCCTATTAAAACTATTATCGAAATTGCTAAAAACGAGGAAAATCAAACCGAAATTCTCAAATTAGCGATCGAAAACATTAAGACTCTATCAACTGTTGGGTCTGGTCAATACATTGCATCGCACTTCTCAACCCATAACGAAGTTGCTATTATTTTCTGTATCTCTTACTTTTTGTATCACTTCAACTTCTTGCACGATGAAAACAAAAAGCAGTTGCTAAAACGTGCCTTTGAAGCAGTTGCTGAAAAAATAGCTGACTACTTAAACGAAAACTAA", "aa": "MEPNNLKEELVSVFEKACSSHKERLDFICSVRESDTFSNVDVPLAPIKTIIEIAKNEENQTEILKLAIENIKTLSTVGSGQYIASHFSTHNEVAIIFCISYFLYHFNFLHDENKKQLLKRAFEAVAEKIADYLNEN", "aa_hexdigest": "3b4796a373f6743d4de66b1311841eeb", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI0000139690", "ncbi_nrp_id": "WP_010874887.1", "uniref100_id": "UniRef100_P75248", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139690", "RefSeq:WP_010874887.1", "UniRef:UniRef100_P75248" ] }, "ips": { "uniref100_id": "UniRef100_P75248", "uniref90_id": "UniRef90_P75248" }, "psc": { "uniref90_id": "UniRef90_P75248", "product": "Uncharacterized protein MG354 homolog", "uniref50_id": "UniRef50_P47596" }, "pscc": { "uniref50_id": "UniRef50_P47596", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47596" ], "product": "Uncharacterized protein MG354" }, "genes": [], "id": "GIHJHMOALN_1142", "locus": "GCA009939745_01141" }, { "type": "cds", "sequence": "contig_1", "start": 646522, "stop": 647646, "strand": "-", "frame": 1, "gene": "clpB", "product": "Chaperone protein ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "GO:0005524", "GO:0005737", "GO:0016887", "GO:0034605", "SO:0001217", "UniRef:UniRef50_P47597", "UniRef:UniRef90_P75247" ], "nt": "TTGCAAACACGCTTAGAGCTCTACCAAACGGAAGGTAACTATGAAGCTGCTTCTAAGATTCTTTACTATGACATCCCCAAACTTAAAAACCAATTAGAACAAGCACAGAAAAAGTATGTTGATTCCAAACATGACTTGTTTAAGACAGAAGTGTCAGAAAACGAAGTGGCCGAAGTAGTATCACAAGCTACCGGTATTCCCTTAAAGAAGCTGTTAGAAACCGAAAAGGAAAAGCTTTTGCATTTAGGTGATGAAATAAAAAAACGGGTTAAGGGTCAAGATGCGGCCGTGGAGACAGTGGTCAATACCGTTATGCGTGGAAGGGTAAACCTCAATGACCCTAACCGGCCAATTGGTTCCTTTATCTTTTTAGGCTCTACTGGTGTTGGTAAAACGGAATTGGCTAAATCACTAGCAGAGGTCTTGTTTGATAATGAAAAAGCGATGATTCGCTTTGACATGAGTGAATACATGGAAAAGCATTCGGTCGCTAAGTTAATCGGAGCGCCTCCTGGATATGTAGGTTATGAACAATCTGGTTTACTAACTGAAGCAGTCCGACGCAAACCTTACTGTGTGCTTCTGTTCGATGAAATTGAAAAAGCACACCCTGATGTAACTAATATTCTTTTACAAGTATTAGATGATGGTACTTTAAAGGACTCTCAAGGTCGTTTAGTTAACTTTAAGAATACGATGATAATTATGACTTCCAACTTGGGTTCCAATTACATTATGGAAAACAAACGCGATTTAGCGATGGAAGCACTAAAAAAACACTTCCGTGCTGAATTTATTAACCGGATTGATGAAATCGTTTTCTTTAGCGTATTGCAAAAAACAACCGTTTTAGAAATTATTACAAATTTATTGGATCAATTAAATCAAAGATTAGCTAAACAAAACTTAAAGTTCACGTTTGACCCTAAGTTAAATGAGTTTATTTACAAATCTAGTTTCGACGAACAGTTTGGTGCTCGACCAATTAAACGTTTTATCGATCGGCAAATAGCTACTCTAATTGCCAAGCAAATTCTGGAAGGTATTATTACTAAAGATGTTAGTTACAACGTAATAGTTGAGAAAGATAAAGTGGCAATAGTAGCCAATAAGGTTAAATCTTAG", "aa": "MQTRLELYQTEGNYEAASKILYYDIPKLKNQLEQAQKKYVDSKHDLFKTEVSENEVAEVVSQATGIPLKKLLETEKEKLLHLGDEIKKRVKGQDAAVETVVNTVMRGRVNLNDPNRPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKAMIRFDMSEYMEKHSVAKLIGAPPGYVGYEQSGLLTEAVRRKPYCVLLFDEIEKAHPDVTNILLQVLDDGTLKDSQGRLVNFKNTMIIMTSNLGSNYIMENKRDLAMEALKKHFRAEFINRIDEIVFFSVLQKTTVLEIITNLLDQLNQRLAKQNLKFTFDPKLNEFIYKSSFDEQFGARPIKRFIDRQIATLIAKQILEGIITKDVSYNVIVEKDKVAIVANKVKS", "aa_hexdigest": "e8ac366f582e392158e748bda5bea9c0", "start_type": "TTG", "rbs_motif": "AAAAA", "psc": { "uniref90_id": "UniRef90_UPI00258F0CA4", "query_cov": 0.9545454545454546, "subject_cov": 0.8419811320754716, "identity": 0.535, "score": 362.0, "evalue": 5.07e-120, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A3D0HNG7" }, "genes": [ "clpB" ], "truncated": "3-prime", "pseudogene": { "start": 646522, "stop": 647946, "observations": { "mutation": [ 647706 ], "stop": [ 647706 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75247", "query_cov": 1.0, "subject_cov": 0.5223463687150838, "identity": 0.997, "score": 709.0, "evalue": 3.17e-252, "gene_start": 1, "gene_end": 374, "reference_start": 343, "reference_end": 716, "reference_sequence": "MDFSFTPTPDKRDFLKEMGRSINDEVLKNKVDPIIGRDNEIRRLIEILSRKNKNNPVLIGEPGVGKTAIVEGFVRRVVNNDVPLNLRDVEIYELSLSGLIAGTQYQGEFEKRVNGILKQVKESNGKIILFIDEIHQIVGLGRNXSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYVEKDGALERRFQKILVSEPSQQEALTIMRGLKTRWELFHNLTIFDSALVAAVEMSARYIPDRNLPDKAIDLIDEASAKIKTEMASEPVVIDTLKREIINLETEYAALKQDKENADNKKKQGHLDNLKQQLDELKKKRDSLTAEWKKEKTNFESINKLKKEIEDLQTRLELYQTEGNYEAASKILYYDIPKLKNQLEQAQKKYVDSKHDLFKTEVSENEVAEVVSQATGIPLKKLLETEKEKLLHLGDEIKKRVKGQDAAVETVVNTVMRGRVNLNDPNRPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKAMIRFDMSEYMEKHSVAKLIGAPPGYVGYEQSGLLTEAVRRKPYCVLLFDEIEKAHPDVTNILLQVLDDGTLKDSQGRLVNFKNTMIIMTSNLGSNYIMENKRDLAMEALKKHFRAEFINRIDEIVFFSVLQKTTVLEIITNLLDQLNQRLAKQNLKFTFDPKLNEFIYKSSFDEQFGARPIKRFIDRQIATLIAKQILEGIITKDVSYNVIVEKDKVAIVANKVKS" }, "paralog": false, "identity": 0.9957805907172996, "score": 909.0, "evalue": 0.0, "description": "Internal stop codon at 647706. Nonsense mutation around 647706.", "psc": { "uniref90_id": "UniRef90_P75247", "gene": "clpB", "product": "Chaperone protein ClpB", "uniref50_id": "UniRef50_P47597", "cog_id": "COG0542", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75247", "UniRef:UniRef50_P47597", "COG:COG0542", "COG:O", "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ] }, "pscc": { "uniref50_id": "UniRef50_P47597", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47597" ], "product": "Chaperone protein ClpB" } }, "id": "GIHJHMOALN_1143", "locus": "GCA009939745_01142" }, { "type": "cds", "sequence": "contig_1", "start": 647704, "stop": 648669, "strand": "-", "frame": 1, "gene": "clpB", "product": "Chaperone protein ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "GO:0005524", "GO:0005737", "GO:0016887", "GO:0034605", "SO:0001217", "UniRef:UniRef50_P47597", "UniRef:UniRef90_P75247" ], "nt": "ATGGATTTTAGTTTTACACCAACACCAGATAAGCGTGATTTCTTAAAGGAAATGGGGCGTAGCATTAACGATGAGGTGCTCAAAAATAAGGTTGATCCTATTATTGGTCGCGACAACGAAATTCGACGCTTAATTGAAATTTTAAGCCGGAAAAACAAGAACAATCCCGTTTTAATTGGTGAACCAGGCGTCGGGAAAACCGCCATTGTTGAAGGATTTGTAAGAAGGGTAGTTAATAATGACGTGCCTTTAAACTTGCGTGATGTTGAAATTTATGAACTTTCGTTGTCTGGTTTAATTGCTGGCACCCAATACCAAGGTGAGTTTGAAAAACGGGTTAACGGTATCTTAAAGCAAGTCAAAGAATCAAATGGCAAGATTATTTTATTCATTGATGAAATCCACCAAATAGTGGGGCTAGGTCGTAACTCCAGTAGTGGTGCCATGGACATTGCCAACATCTTAAAACCAATGTTAGCACGCGGTGAAATTAAGGTTATTGGTGCCACGACACTGAAAGAGTACCGTGAATATGTGGAGAAGGACGGCGCTTTAGAACGGCGCTTCCAGAAGATCCTTGTCAGTGAACCGAGTCAACAAGAAGCACTTACCATTATGCGGGGTTTGAAGACCCGTTGGGAACTTTTTCATAACCTCACCATCTTTGACAGTGCCTTGGTAGCTGCTGTAGAAATGTCAGCACGTTATATTCCTGATCGTAATTTACCCGATAAAGCGATTGATTTAATTGATGAAGCTAGTGCCAAGATTAAAACGGAAATGGCTTCAGAGCCAGTGGTAATTGACACCCTTAAGCGGGAAATTATTAACTTAGAAACTGAATATGCCGCTTTAAAACAAGATAAGGAAAATGCTGATAACAAGAAAAAACAAGAACACTTAGACAATCTAAAACAACAGTTAGATGAGTTAAAGAAAAAGCGTGATTCTCTAACTGCCGAATGA", "aa": "MDFSFTPTPDKRDFLKEMGRSINDEVLKNKVDPIIGRDNEIRRLIEILSRKNKNNPVLIGEPGVGKTAIVEGFVRRVVNNDVPLNLRDVEIYELSLSGLIAGTQYQGEFEKRVNGILKQVKESNGKIILFIDEIHQIVGLGRNSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYVEKDGALERRFQKILVSEPSQQEALTIMRGLKTRWELFHNLTIFDSALVAAVEMSARYIPDRNLPDKAIDLIDEASAKIKTEMASEPVVIDTLKREIINLETEYAALKQDKENADNKKKQEHLDNLKQQLDELKKKRDSLTAE", "aa_hexdigest": "9470523dff3ac16744684dd38a509faf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_U4KPM2", "query_cov": 0.8535825545171339, "subject_cov": 0.9415807560137457, "identity": 0.588, "score": 314.0, "evalue": 1.1e-103, "valid": false, "product": "ATP-dependent Clp protease (ATP-binding subunit, clpB), 1", "uniref50_id": "UniRef50_A0A930RWV4" }, "genes": [ "clpB" ], "truncated": "5-prime", "pseudogene": { "start": 647401, "stop": 648669, "observations": { "mutation": [ 647706 ], "stop": [ 647706 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75247", "query_cov": 1.0031152647975077, "subject_cov": 0.44972067039106145, "identity": 0.9940000000000001, "score": 599.0, "evalue": 6.63e-210, "gene_start": 1, "gene_end": 321, "reference_start": 1, "reference_end": 322, "reference_sequence": "MDFSFTPTPDKRDFLKEMGRSINDEVLKNKVDPIIGRDNEIRRLIEILSRKNKNNPVLIGEPGVGKTAIVEGFVRRVVNNDVPLNLRDVEIYELSLSGLIAGTQYQGEFEKRVNGILKQVKESNGKIILFIDEIHQIVGLGRNXSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYVEKDGALERRFQKILVSEPSQQEALTIMRGLKTRWELFHNLTIFDSALVAAVEMSARYIPDRNLPDKAIDLIDEASAKIKTEMASEPVVIDTLKREIINLETEYAALKQDKENADNKKKQGHLDNLKQQLDELKKKRDSLTAEWKKEKTNFESINKLKKEIEDLQTRLELYQTEGNYEAASKILYYDIPKLKNQLEQAQKKYVDSKHDLFKTEVSENEVAEVVSQATGIPLKKLLETEKEKLLHLGDEIKKRVKGQDAAVETVVNTVMRGRVNLNDPNRPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKAMIRFDMSEYMEKHSVAKLIGAPPGYVGYEQSGLLTEAVRRKPYCVLLFDEIEKAHPDVTNILLQVLDDGTLKDSQGRLVNFKNTMIIMTSNLGSNYIMENKRDLAMEALKKHFRAEFINRIDEIVFFSVLQKTTVLEIITNLLDQLNQRLAKQNLKFTFDPKLNEFIYKSSFDEQFGARPIKRFIDRQIATLIAKQILEGIITKDVSYNVIVEKDKVAIVANKVKS" }, "paralog": false, "identity": 0.9929078014184397, "score": 805.0, "evalue": 2.94e-293, "description": "Internal stop codon at 647706. Nonsense mutation around 647706.", "psc": { "uniref90_id": "UniRef90_P75247", "gene": "clpB", "product": "Chaperone protein ClpB", "uniref50_id": "UniRef50_P47597", "cog_id": "COG0542", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75247", "UniRef:UniRef50_P47597", "COG:COG0542", "COG:O", "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ] }, "pscc": { "uniref50_id": "UniRef50_P47597", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47597" ], "product": "Chaperone protein ClpB" } }, "id": "GIHJHMOALN_1144", "locus": "GCA009939745_01143" }, { "type": "cds", "sequence": "contig_1", "start": 648845, "stop": 648964, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCAAACACAGCAAATTATTGATTACAAAAAGTGGGTGCAAAAGCGCTTGAACTACTTTTTAAAGGCACTGTTCCCTAACACTTGGAAGATTGCCAAAACAATTCCGTTGTACTAG", "aa": "MPQTQQIIDYKKWVQKRLNYFLKALFPNTWKIAKTIPLY", "aa_hexdigest": "84e24c4ca8c6d5540c98f8f0f6fabad4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4827.7321, "isoelectric_point": 10.09466915130615 }, "id": "GIHJHMOALN_1145", "locus": "GCA009939745_01144" }, { "type": "cds", "sequence": "contig_1", "start": 648974, "stop": 649246, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01633.27" ], "nt": "GTGGAATTAACAGATAAACACAAAGCGATTCCCAAAACCTTGTGTCACCACGATTCCACCTTTGATAATTTGGTTTATACCCCAAAAAAGCAGGTCGTTTTAATTGACTTTGAGTGGTCTTGTGTGGACAACCCTTACTATGAGATTGCCAACATTATTCGCGAAGAGTTGACAATTGAAACAGCCAACCAAATTATTGACTTGTACGGTAGCTTAGACAAAAAACTAGTTTTTGAAACGGTAATTTACGTGTTGCTGTTTGCCTTTCAGTGA", "aa": "MELTDKHKAIPKTLCHHDSTFDNLVYTPKKQVVLIDFEWSCVDNPYYEIANIIREELTIETANQIIDLYGSLDKKLVFETVIYVLLFAFQ", "aa_hexdigest": "a90ae292dc3c2d7c044b849a270f7ab7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01633.27", "name": "Choline_kinase", "length": 47, "aa_cov": 0.5111111111111111, "hmm_cov": 1.0, "evalue": 4.61008884322878e-09, "score": 31.452850341796875, "start": 11, "stop": 56 } ], "seq_stats": { "molecular_weight": 10554.03859999999, "isoelectric_point": 4.813148689270019 }, "id": "GIHJHMOALN_1146", "locus": "GCA009939745_01145" }, { "type": "cds", "sequence": "contig_1", "start": 649334, "stop": 649693, "strand": "-", "frame": 3, "gene": "cotS", "product": "putative MG356-like protein", "db_xrefs": [ "COG:COG0510", "COG:H", "SO:0001217", "UniRef:UniRef50_Q49423", "UniRef:UniRef90_P75246" ], "nt": "ATGAATAAGGGCGATGCAATTGCTTTTCTTTGTGAAAAGTTAAACCTCCAACCAACTGCTGTAGCTCATGTGGAACAAATCCACTCGGGTTTTACTAATTTTTCCTTTTTTACAGAACTGAAAGATGGGAGTAAGTACCAAGTCAGGTTAGCGCGTAAAGATGTACCGTTGAACCGACGCAACGAGCAAATTATCCTGGACTTAGTGACCCCCATCTTTGGTAACCCCTTTGTTTACTTTGATGTAACTACCGGTAATGCCATTAAGAAGTGGATTGAAGGAAAACAACCAAAACGTCCCACAAGGCTGTTTTTAGAACAACTAGTGGCTTCTTTAAAAAAAGTACATGCAGTCGAGTGA", "aa": "MNKGDAIAFLCEKLNLQPTAVAHVEQIHSGFTNFSFFTELKDGSKYQVRLARKDVPLNRRNEQIILDLVTPIFGNPFVYFDVTTGNAIKKWIEGKQPKRPTRLFLEQLVASLKKVHAVE", "aa_hexdigest": "0de0c28ea31076910975993e6ffed7d8", "start_type": "ATG", "rbs_motif": null, "genes": [ "cotS" ], "truncated": "5-prime", "pseudogene": { "start": 649031, "stop": 649693, "observations": { "mutation": [ 649312, 649336 ], "stop": [ 649312, 649336 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75246", "query_cov": 1.0, "subject_cov": 0.4219858156028369, "identity": 0.983, "score": 238.0, "evalue": 3.75e-77, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MNKGDAIDFLCEKLNLQPTAVAHVEQIHSGFTNFSFFTELKDGSKYQVRLARKDVPLNRRNEQIILDLVTPIFGNPFVYFDVTAGNAIKKWIEGKQPKRPTRLFLEQLVASLKKVHAVEWKQFANEIWIFDPLIYFNQTKLPDFYKKLYVELTDKHKAIPKTLCHHDSTFDNLVYTPKKQVVLIDFEWSCVDNPYYEIANIIREELTIETANQIIDLYGSLDKKLVFETVIYVLLFAFQWTEIMPQTQQIIDYKKWVQKRLNYFLKALFPNTWKIAKTIPLY" }, "paralog": false, "identity": 0.9818181818181818, "score": 439.0, "evalue": 1.13e-158, "description": "Internal stop codon at 649312, 649336. Nonsense mutation around 649312, 649336.", "psc": { "uniref90_id": "UniRef90_P75246", "gene": "CotS", "product": "Uncharacterized protein MG356 homolog", "uniref50_id": "UniRef50_Q49423", "cog_id": "COG0510", "cog_category": "H", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75246", "UniRef:UniRef50_Q49423", "COG:COG0510", "COG:H" ] }, "pscc": { "uniref50_id": "UniRef50_Q49423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49423" ], "product": "Uncharacterized protein MG356" } }, "id": "GIHJHMOALN_1147", "locus": "GCA009939745_01146" }, { "type": "cds", "sequence": "contig_1", "start": 649701, "stop": 650873, "strand": "-", "frame": 2, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "GO:0000287", "GO:0005524", "GO:0005829", "GO:0006083", "GO:0006085", "GO:0008776", "KEGG:K00925", "RefSeq:WP_014325588.1", "SO:0001217", "UniParc:UPI0001DD6424", "UniRef:UniRef100_A0AB38W6H5", "UniRef:UniRef50_P75245", "UniRef:UniRef90_P75245" ], "nt": "ATGAACGACAACAAAATTTTAGTAGTCAATGCTGGCAGTAGCTCGATCAAATTCCAATTGTTTGATTATCACAAAAAAGTCCTAGCTAAAGCCCTTTGTGAACGAATTTTTGTCGATGGTTTTTTCAAACTTGAATTTAACGAACAGAAGGTTGAAGAAAAAGTAGCTTTCCCCGACCACCACGCCGCTGTAACCCACTTTTTAAACACACTTAAAAAGCACAAAATTATCCAGGAACTTAGTGACATTACTCTAGTTGGTCACCGCGTGGTGCAGGGCGCTAACTACTTTAAGGATTCGGTGATCGTAGATGCAGCAGCACTCGCTAAAATTAAGGAATTTATTAAGTTAGCGCCACTGCACAACAAGCCAGAGGCTGATGTGATTGAAATCTTTTTCAAAGAAGTTCCTAGTGCTAAGAATGTGGCAGTCTTTGACACAACCTTTCACACTACCATTCCCCAAGAAAACTACTTGTATGCGGTACCACGTTCATGGGAGCAAAAACACTTAGTACGTCGTTATGGCTTCCATGGTACTTCTTACAAGTTCATTAATAACTATTTAGAAAAGCACTTGAACAAGCAAAACCTGAACCTGATTGTGTGTCACTTGGGTAACGGTGCGAGTGTTTGTGCCATTAAGAATGGTAAGTCATTTAATACTTCAATGGGATTCACCCCGCTAGAAGGTCTTATTATGGGAACCCGTAGTGGTGACCTTGACCCTGCCATTATTGGTTATGTAGCAGAACAGGAAAACATGAGTGCTAGTGATGTGGTCAATGCGCTTAATAAAAAATCAGGGATGTTGGCGTTAACTGGTGCATCGGATATGCGTGATGTTTTCGCTAAACCCCAAGAAAACGCTGTGGCTATCAAAATGTATGTTAACCGGGTAGCGGATTACATTGCTAAATACTTGAACCAATTGGAAGGCAACATTGATGGTTTAGTGTTTACTGGTGGCATTGGCGAGAATGCTAGTGATTGTGTAGAGTTGTTTATTAACGCCGTTAAATCATTAGGCTTTGCGACTGATCTAAAACTGTTTGTCAAGTACGGTGATAGCTGTGTGGTATCCACGCCCCAAAGTAAGTACAAGATTTACCGGGTACGTACTAATGAAGAGTTAATGATTGTGGAAGACTCCATTCGACTAACTCAAAAATAG", "aa": "MNDNKILVVNAGSSSIKFQLFDYHKKVLAKALCERIFVDGFFKLEFNEQKVEEKVAFPDHHAAVTHFLNTLKKHKIIQELSDITLVGHRVVQGANYFKDSVIVDAAALAKIKEFIKLAPLHNKPEADVIEIFFKEVPSAKNVAVFDTTFHTTIPQENYLYAVPRSWEQKHLVRRYGFHGTSYKFINNYLEKHLNKQNLNLIVCHLGNGASVCAIKNGKSFNTSMGFTPLEGLIMGTRSGDLDPAIIGYVAEQENMSASDVVNALNKKSGMLALTGASDMRDVFAKPQENAVAIKMYVNRVADYIAKYLNQLEGNIDGLVFTGGIGENASDCVELFINAVKSLGFATDLKLFVKYGDSCVVSTPQSKYKIYRVRTNEELMIVEDSIRLTQK", "aa_hexdigest": "26ef898ff74f45ad64f2b172e0a811ad", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6424", "ncbi_nrp_id": "WP_014325588.1", "uniref100_id": "UniRef100_A0AB38W6H5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6424", "RefSeq:WP_014325588.1", "UniRef:UniRef100_A0AB38W6H5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6H5", "uniref90_id": "UniRef90_P75245" }, "psc": { "uniref90_id": "UniRef90_P75245", "gene": "ackA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_P75245", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925", "go_ids": [ "GO:0005829", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ] }, "pscc": { "uniref50_id": "UniRef50_P75245", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75245" ], "product": "Acetate kinase" }, "genes": [ "ackA" ], "id": "GIHJHMOALN_1148", "locus": "GCA009939745_01147" }, { "type": "cds", "sequence": "contig_1", "start": 650942, "stop": 651112, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XFQ7", "UniRef:UniRef90_A0AAN4XFQ7" ], "nt": "ATGAGTGCTAAAACTTCATATATTGTGGCTGCTGTTTCGACAGCTGTAGGTATTATTCTCTACTTTATCTCTTCCCTCATGGGGGTAATTGGTGTAAGTAATGATAAGACCTTAATCATTGTCTTTTTTGTATTAGCTTCAATCATCATTATTAACGCCTGTGTTTTTTGA", "aa": "MSAKTSYIVAAVSTAVGIILYFISSLMGVIGVSNDKTLIIVFFVLASIIIINACVF", "aa_hexdigest": "83b14a31674ea43c546bcd3a635081ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAN4XFQ7", "query_cov": 1.0, "subject_cov": 0.8115942028985508, "identity": 1.0, "score": 94.4, "evalue": 2.28e-24, "valid": true, "uniref50_id": "UniRef50_A0AAN4XFQ7" }, "pscc": { "uniref50_id": "UniRef50_A0AAN4XFQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XFQ7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5899.115099999997, "isoelectric_point": 7.915057563781738 }, "id": "GIHJHMOALN_1149", "locus": "GCA009939745_01148" }, { "type": "cds", "sequence": "contig_1", "start": 651214, "stop": 651636, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_534", "db_xrefs": [ "RefSeq:WP_010874891.1", "SO:0001217", "UniParc:UPI000013AD23", "UniRef:UniRef100_P75244", "UniRef:UniRef50_P75244", "UniRef:UniRef90_P75244" ], "nt": "ATGAATACTTTTAAAGAAACCCTCTTTTCATTAAAGAAAAGTAATTATTTGTTGAATGAGAGTGCTCAAATTCTCAATGATTGTGTAGTAAATAACAATGAAATCGATGAACGCTTACGCAATTCGATTAAATTTGCCCTTGGCATTATTAATTTAGCTAGTTACTGTCTTAAAAACGAACAAGAACAGCTAGACCATGAACTCAACCATGGTAATGAAGCGCCCTGGGAGTTTAGTTTCAATGAAGCTGAACAACTAATAGAATGCACTGTCAAAAATAACTTTGGCAACGAAAAACTAGTGGATTTAATTTTCCACATTGGTGATGCCATGGAGACTTACCGTACCACTAATATTAAGTTTAGAGTACCCAAGTCTTACTATGATGCCAAACAGCAAATTAGAAAGGTAATTAAAAACTAA", "aa": "MNTFKETLFSLKKSNYLLNESAQILNDCVVNNNEIDERLRNSIKFALGIINLASYCLKNEQEQLDHELNHGNEAPWEFSFNEAEQLIECTVKNNFGNEKLVDLIFHIGDAMETYRTTNIKFRVPKSYYDAKQQIRKVIKN", "aa_hexdigest": "19281e5bfc309493ec36856b74870de5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AD23", "ncbi_nrp_id": "WP_010874891.1", "uniref100_id": "UniRef100_P75244", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AD23", "RefSeq:WP_010874891.1", "UniRef:UniRef100_P75244" ] }, "ips": { "uniref100_id": "UniRef100_P75244", "uniref90_id": "UniRef90_P75244" }, "psc": { "uniref90_id": "UniRef90_P75244", "product": "Uncharacterized protein MPN_534", "uniref50_id": "UniRef50_P75244" }, "pscc": { "uniref50_id": "UniRef50_P75244", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75244" ], "product": "Uncharacterized protein MPN_534" }, "genes": [], "id": "GIHJHMOALN_1150", "locus": "GCA009939745_01149" }, { "type": "cds", "sequence": "contig_1", "start": 651642, "stop": 652262, "strand": "+", "frame": 3, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518", "RefSeq:WP_010874892.1", "SO:0001217", "UniParc:UPI0000135351", "UniRef:UniRef100_P75243", "UniRef:UniRef50_Q49424", "UniRef:UniRef90_P75243" ], "nt": "ATGATTGCGTCAATTTTTGGAAAAATTACCTTTGTTGGTAAGCGAAAAATTATTGTTGAAGCCAATTGTATTTCCTATTGGTTTAATGTGAAAGAAAACCATAGCTTCGAAAAGAACTTGGAAAAACCGCGCCAGGTCTTCTGTCAGATCATTAAAAGAATGGTTACGAACCAGATCTTGGAAGAGGGCTTTGCCTTCAATACCTTAGAGGAAAAGGAATGGTTTTCGAAGTTCATCGAACTCAATGGCATTGGTAGTAAAACTGCTTTAAACTTGCTCAATAACAACCTCGAGGAAATGCGTGATTATATTAAAAACAGTAACTACCATGCCTTGACTAAGTTAACTGGTTCTAACAGTAAGGTAGCTCGCGCATTGCTGGCACTGGAACTGTATGACCGAGACGATGGTGGTAAACGGATTAAACCCAACACTGCCATGGCCAATGACTATGATGAGATGTTTGACACCTTAAAGTCGTTGGGCTATAAACCCCAAGATATCCAAAACGCTTTGAGCAAAATTGAAATTAAACCTAACTTTGATGTAAGTGAAGTGATTGCTGAAGTGATTAAGTTAATGTCTTTCCAGAACAATGAAGTTACAAATAAAACCGCCTAA", "aa": "MIASIFGKITFVGKRKIIVEANCISYWFNVKENHSFEKNLEKPRQVFCQIIKRMVTNQILEEGFAFNTLEEKEWFSKFIELNGIGSKTALNLLNNNLEEMRDYIKNSNYHALTKLTGSNSKVARALLALELYDRDDGGKRIKPNTAMANDYDEMFDTLKSLGYKPQDIQNALSKIEIKPNFDVSEVIAEVIKLMSFQNNEVTNKTA", "aa_hexdigest": "b50832c041587a46bea8bff4b6db39d6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135351", "ncbi_nrp_id": "WP_010874892.1", "uniref100_id": "UniRef100_P75243", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135351", "RefSeq:WP_010874892.1", "UniRef:UniRef100_P75243" ] }, "ips": { "uniref100_id": "UniRef100_P75243", "uniref90_id": "UniRef90_P75243" }, "psc": { "uniref90_id": "UniRef90_P75243", "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_Q49424", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49424" ], "product": "Holliday junction branch migration complex subunit RuvA" }, "genes": [ "ruvA" ], "id": "GIHJHMOALN_1151", "locus": "GCA009939745_01150" }, { "type": "cds", "sequence": "contig_1", "start": 652237, "stop": 653160, "strand": "+", "frame": 1, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.-", "EC:3.6.4.12", "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "RefSeq:WP_014325589.1", "SO:0001217", "UniParc:UPI0001DD6426", "UniRef:UniRef100_A0AAV5NA15", "UniRef:UniRef50_Q49425", "UniRef:UniRef90_P75242" ], "nt": "ATGAAGTTACAAATAAAACCGCCTAACAATTTCGCTGAGTTTGTCGGTAAACAAGAAATTATTAACCAAATTCAACTCAGCATTAAAGCGTCCCGTATCAATAAGGCGCAGTTGGACCACATTCTTTTATACGGTCCACCTGGCGTTGGTAAAACCACATTAGCTCGCTTAATTGCGAGTGAAATGAACACCAAACTGCAAATTATTCAGGGTGGGCACTTACAGCGACCGAGTGATTTTCTCAACGCCGTTTCCCTCATTAAAAAGGGTGATGTACTGTTTGTGGATGAAATCCATGCAGTAGCACCCAGTGTGATGGAGTTAATGTTTCCCGTGATGGATGATTTTCGCGTCCAAGTCTTAATTGGCAAGGACTTTAATTCCAAGATGGTGGAAATGAAAGTTAATCCGTTTACCTTAATCGGAGCCACCACTCAGTTCGGTAAGATTATCAACCCGCTGGAAGATCGCTTTGGGATGATCCTTAACATTGACTACTACAGTAACCAAGAGATCGAGCGGATTGTAAGCATCTATGGTGAGCAAATGGAGTTGGAATTAAAACCGGAAGAAATTACCCAGATTACCCAACACAGTAAGCAAACCCCGCGGATTGCCATTCGAATTGTCAAGCGTTTGTTTGAACAGAAAATAGTCAACAAAAAGATTGATCTGGCTGCTCTGTTCAAAAGCTTAATGATTTACAAAAATGGTTTACAGAGCATTGATGTGCAATATCTCAAAGCACTCAACGGGCAGTACGAGCCTCAGGGTATTAAGTCAATTTGTTCGATGCTAGGGATTGATAAATCCACTGTCGAAAACAAGATTGAGCCCTTCTTGCTGCGGGAAAACATGATCCAAAAGACCAAGAAAGGGCGGATCATAACCCGCACTGGGCGTAATTATTTAACTAGCTGCTAA", "aa": "MKLQIKPPNNFAEFVGKQEIINQIQLSIKASRINKAQLDHILLYGPPGVGKTTLARLIASEMNTKLQIIQGGHLQRPSDFLNAVSLIKKGDVLFVDEIHAVAPSVMELMFPVMDDFRVQVLIGKDFNSKMVEMKVNPFTLIGATTQFGKIINPLEDRFGMILNIDYYSNQEIERIVSIYGEQMELELKPEEITQITQHSKQTPRIAIRIVKRLFEQKIVNKKIDLAALFKSLMIYKNGLQSIDVQYLKALNGQYEPQGIKSICSMLGIDKSTVENKIEPFLLRENMIQKTKKGRIITRTGRNYLTSC", "aa_hexdigest": "998350c82dbb5f055ce8de0f8252ab90", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6426", "ncbi_nrp_id": "WP_014325589.1", "uniref100_id": "UniRef100_A0AAV5NA15", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6426", "RefSeq:WP_014325589.1", "UniRef:UniRef100_A0AAV5NA15" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NA15", "uniref90_id": "UniRef90_P75242" }, "psc": { "uniref90_id": "UniRef90_P75242", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.-", "3.6.4.12" ], "uniref50_id": "UniRef50_Q49425", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49425" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "GIHJHMOALN_1152", "locus": "GCA009939745_01151" }, { "type": "cds", "sequence": "contig_1", "start": 653146, "stop": 653808, "strand": "-", "frame": 1, "gene": "dinP", "product": "putative MG360-like protein", "db_xrefs": [ "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0005829", "GO:0009432", "GO:0042276", "SO:0001217", "UniRef:UniRef50_P75241", "UniRef:UniRef90_P75241" ], "nt": "GTGGAACTTCCTGGGATAGGCAAACGACAACTTGACAACGCCTTTAAAAATAATTTTCACAAGATTGGAGACCTCGCTAAGTGTAAAGATGTGACCTTGTTCAAACGGGTGTTTGGTATTGCGTGGGAAAGCTTGCACGCTGTGGCTTTGGGTGAAACTTATACGCAAAGCGAGCAGGATGTCAAAAGTCGCTCAATTGCTGTAAGTGAAACGCTGGAAGATTTGAACTACTCTTCCAATCAACTCCAGCAAAAACTCACTAGTATTTTCAACGAGTTGTATGCTAGGTTACAACTGTCCTTTCAAATGTGCAAGGGAGTAGTGGTGCAGTTAAAGAGCAATGACTTTATTGTCAACTCCCACTCTCAATCGATCAAGAAATACACCGCTGATTACCAAACCCTACTCGTAATAGTCAAAAAGCTCTTTAACAGGCTATTAATGGGTGTGGGGCTTAACATCCGCTTAATTGGCGTGAGTTTTTTTGGATTAAAAAATAACCCCTCCAGTTCAAGACCAGAAGGGTTACTTTTTTATGAGTATCAGCAAGCTAAGCCTAAACAACAGACTGCTCACTTTGCTTTAGATCAAATGATCTATGAGATTAACCAAAGCTTTGGTTATGAGATTATCCAGCGTGCCAAAAAGTTAGCAGCTAGTTAA", "aa": "MELPGIGKRQLDNAFKNNFHKIGDLAKCKDVTLFKRVFGIAWESLHAVALGETYTQSEQDVKSRSIAVSETLEDLNYSSNQLQQKLTSIFNELYARLQLSFQMCKGVVVQLKSNDFIVNSHSQSIKKYTADYQTLLVIVKKLFNRLLMGVGLNIRLIGVSFFGLKNNPSSSRPEGLLFYEYQQAKPKQQTAHFALDQMIYEINQSFGYEIIQRAKKLAAS", "aa_hexdigest": "90bf47b9eb9554f52e98fc31ac938fa3", "start_type": "GTG", "rbs_motif": null, "genes": [ "dinP" ], "truncated": "3-prime", "pseudogene": { "start": 653146, "stop": 654108, "observations": { "mutation": [ 653823 ], "stop": [ 653823 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75241", "query_cov": 1.0, "subject_cov": 0.5339805825242718, "identity": 0.991, "score": 422.0, "evalue": 2.87e-146, "gene_start": 1, "gene_end": 220, "reference_start": 193, "reference_end": 412, "reference_sequence": "MIGTFTYLDPTIQADPNLLFFYFDFDAFFASVEEIENPELKNQPLIVGNRTSRSVVSTCNYLARSYGIKSGMPIAKALELCPQAIFATSHFRNYRKYSAKIFAMIAEQFNLEVHTLSIDEGFVCFRDLSPRKAFSLAKRIQRHVYEQLNFHISIGISNQFTLAKIFSNQAKPFGVKSCFSKEVKRKLWPLPIVELPGIGKRQLDNAFKNNFHKIGDLAKCKDVTLFKRVFGIAWESLHAVALGETYTQSEQDVKSRSIAVSETLEYLNYSSNQLQQKLTSIFNELYARLQLSFQMCKGVVVQLKSNDFIVNSHSQSIKKYTADYQTLLVIVKKLFNRLLMGVGLNIRLIGVSFFGLKNNPSSSRPEGLLFYEYQQAKPKQQTAHFALDQMIYEINQSFGYEIIQRAKKLAAS" }, "paralog": false, "identity": 0.99375, "score": 623.0, "evalue": 1.16e-227, "description": "Internal stop codon at 653823. Nonsense mutation around 653823.", "psc": { "uniref90_id": "UniRef90_P75241", "gene": "DinP", "product": "Uncharacterized protein MG360 homolog", "uniref50_id": "UniRef50_P75241", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75241", "UniRef:UniRef50_P75241", "COG:COG0389", "COG:L", "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75241", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75241" ], "product": "Uncharacterized protein MG360 homolog" } }, "id": "GIHJHMOALN_1153", "locus": "GCA009939745_01152" }, { "type": "cds", "sequence": "contig_1", "start": 653821, "stop": 654384, "strand": "-", "frame": 1, "gene": "dinP", "product": "putative MG360-like protein", "db_xrefs": [ "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0005829", "GO:0009432", "GO:0042276", "SO:0001217", "UniRef:UniRef50_P75241", "UniRef:UniRef90_P75241" ], "nt": "ATGATCGGCACTTTTACATATTTAGATCCAACCATACAAGCTGATCCTAATCTGCTTTTTTTCTATTTTGATTTTGATGCCTTTTTTGCATCAGTAGAAGAGATAGAAAACCCCGAGCTCAAAAATCAACCTTTAATTGTCGGTAACCGGACATCTAGGAGTGTGGTGTCGACTTGTAATTACCTTGCCCGGAGCTATGGCATTAAGTCGGGCATGCCAATTGCTAAGGCCTTGGAATTGTGTCCCCAAGCGATCTTTGCCACATCACACTTTCGTAACTACCGCAAGTATTCCGCTAAGATCTTTGCCATGATTGCTGAGCAGTTTAACCTAGAGGTACACACCCTTTCGATTGATGAGGGCTTTGTGTGTTTTCGTGACCTTTCACCCCGTAAAGCATTTAGTTTGGCTAAACGGATTCAGCGCCACGTTTATGAACAGTTAAACTTTCACATCTCCATTGGTATTTCCAACCAATTTACCCTAGCGAAAATCTTTTCCAATCAGGCTAAACCGTTTGGGGTTAAAAGCTGTTTTAGCAAGGAAGTTAAACGCAAACTCTGA", "aa": "MIGTFTYLDPTIQADPNLLFFYFDFDAFFASVEEIENPELKNQPLIVGNRTSRSVVSTCNYLARSYGIKSGMPIAKALELCPQAIFATSHFRNYRKYSAKIFAMIAEQFNLEVHTLSIDEGFVCFRDLSPRKAFSLAKRIQRHVYEQLNFHISIGISNQFTLAKIFSNQAKPFGVKSCFSKEVKRKL", "aa_hexdigest": "ef81b4fc58cc981941b32eeebdbd9fa7", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "dinP" ], "truncated": "5-prime", "pseudogene": { "start": 653518, "stop": 654384, "observations": { "mutation": [ 653823 ], "stop": [ 653823 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75241", "query_cov": 1.0, "subject_cov": 0.4538834951456311, "identity": 1.0, "score": 374.0, "evalue": 1.04e-127, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MIGTFTYLDPTIQADPNLLFFYFDFDAFFASVEEIENPELKNQPLIVGNRTSRSVVSTCNYLARSYGIKSGMPIAKALELCPQAIFATSHFRNYRKYSAKIFAMIAEQFNLEVHTLSIDEGFVCFRDLSPRKAFSLAKRIQRHVYEQLNFHISIGISNQFTLAKIFSNQAKPFGVKSCFSKEVKRKLWPLPIVELPGIGKRQLDNAFKNNFHKIGDLAKCKDVTLFKRVFGIAWESLHAVALGETYTQSEQDVKSRSIAVSETLEYLNYSSNQLQQKLTSIFNELYARLQLSFQMCKGVVVQLKSNDFIVNSHSQSIKKYTADYQTLLVIVKKLFNRLLMGVGLNIRLIGVSFFGLKNNPSSSRPEGLLFYEYQQAKPKQQTAHFALDQMIYEINQSFGYEIIQRAKKLAAS" }, "paralog": false, "identity": 0.9930555555555556, "score": 567.0, "evalue": 2.61e-206, "description": "Internal stop codon at 653823. Nonsense mutation around 653823.", "psc": { "uniref90_id": "UniRef90_P75241", "gene": "DinP", "product": "Uncharacterized protein MG360 homolog", "uniref50_id": "UniRef50_P75241", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75241", "UniRef:UniRef50_P75241", "COG:COG0389", "COG:L", "GO:0005829", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75241", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75241" ], "product": "Uncharacterized protein MG360 homolog" } }, "id": "GIHJHMOALN_1154", "locus": "GCA009939745_01153" }, { "type": "cds", "sequence": "contig_1", "start": 654502, "stop": 654987, "strand": "+", "frame": 1, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0070180", "KEGG:K02864", "RefSeq:WP_010874895.1", "SO:0001217", "UniParc:UPI0000133B3C", "UniRef:UniRef100_P75240", "UniRef:UniRef50_P36263", "UniRef:UniRef90_P75240" ], "nt": "ATGGAAGCTAAAAAAGATAAAGCTCAGCAGGTTGCTGATGTCTCCCATTTGCTTTCAACCAGTGCAGGATTTGTCATTTTTGACTATACCAGCATGTCAGCAATTGAAGCGACTTCTATTCGCAAGAAGTTGTTTAAGAATGGCAGCAAAATTAAGGTAATTAAAAACAATATTTTGCGCCGTGCTTTAAAAGCTGGTAAGTTTGAAGGAATTGACGAAACTGCCATTAAAGGCAAATTGGCGGTTGCTGTTGGAGTGAACGAAATTGTAGAAACGTTGAAAGCTGTTGACGGTGTGGTAAAGGCGAAGGAAGCGATGAACTTTGTTTGTGGTTACTTTGACAACCGTGCTTTTAACAGTGCTGATTTAGAGAAGATTGCAAAACTGCCAGGACGAAACGAACTTTATGGGATGTTTCTCTCGGTATTACAAGCTCCATTGCGCAAGTTCTTATACGCTTTGGAAGCAGTTAAGGCTGCAAAATAA", "aa": "MEAKKDKAQQVADVSHLLSTSAGFVIFDYTSMSAIEATSIRKKLFKNGSKIKVIKNNILRRALKAGKFEGIDETAIKGKLAVAVGVNEIVETLKAVDGVVKAKEAMNFVCGYFDNRAFNSADLEKIAKLPGRNELYGMFLSVLQAPLRKFLYALEAVKAAK", "aa_hexdigest": "82648b8a97d4dd48affe0eb54c8d0619", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133B3C", "ncbi_nrp_id": "WP_010874895.1", "uniref100_id": "UniRef100_P75240", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133B3C", "RefSeq:WP_010874895.1", "UniRef:UniRef100_P75240" ] }, "ips": { "uniref100_id": "UniRef100_P75240", "uniref90_id": "UniRef90_P75240" }, "psc": { "uniref90_id": "UniRef90_P75240", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_P36263", "cog_id": "COG0244", "cog_category": "J", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P36263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36263" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "GIHJHMOALN_1155", "locus": "GCA009939745_01154" }, { "type": "cds", "sequence": "contig_1", "start": 655025, "stop": 655393, "strand": "+", "frame": 2, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_014325591.1", "SO:0001217", "UniParc:UPI0001DD6428", "UniRef:UniRef100_A0AAP8E540", "UniRef:UniRef50_P36255", "UniRef:UniRef90_P36255" ], "nt": "ATGGCAAAACTAGATAAAAACCAATTAATTGAATCGTTGAAGGAAATGACCATCATGGAAATCGATGAAATCATTAAGGCTGTAGAAGAAGCTTTTGGAGTATCGGCAACACCTGTAGTAGCTGCTGGTGCTGTTGGTGGTACACAAGAAGCTGCTAGCGAAGTGACTGTGAAAGTTACTGGTTACGCTGACAACGCTAAATTAGCTGTGTTAAAGCTTTACCGCGAAATTGCTGGTGTTGGTTTAATGGAAGCTAAAACTGCTGTGGAAAAACTTCCTTGTGTTGTTAAGCAAGACATCAAACCTGAAGAAGCTGAAGAACTTAAAAAGCGTTTCGTTGAAGTTGGAGCAACTGTTGAAATCAAATAA", "aa": "MAKLDKNQLIESLKEMTIMEIDEIIKAVEEAFGVSATPVVAAGAVGGTQEAASEVTVKVTGYADNAKLAVLKLYREIAGVGLMEAKTAVEKLPCVVKQDIKPEEAEELKKRFVEVGATVEIK", "aa_hexdigest": "a01200b5df09d6b30a7501353e9cf82c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6428", "ncbi_nrp_id": "WP_014325591.1", "uniref100_id": "UniRef100_A0AAP8E540", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6428", "RefSeq:WP_014325591.1", "UniRef:UniRef100_A0AAP8E540" ] }, "ips": { "uniref100_id": "UniRef100_A0AAP8E540", "uniref90_id": "UniRef90_P36255" }, "psc": { "uniref90_id": "UniRef90_P36255", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_P36255", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P36255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36255" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "GIHJHMOALN_1156", "locus": "GCA009939745_01155" }, { "type": "cds", "sequence": "contig_1", "start": 655583, "stop": 655846, "strand": "-", "frame": 3, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02968", "RefSeq:WP_014325593.1", "SO:0001217", "UniParc:UPI0002386BED", "UniRef:UniRef100_A0AAX0S2C1", "UniRef:UniRef50_P55750", "UniRef:UniRef90_P75237" ], "nt": "ATGGCTAATATCAAATCGAACGAAAAACGTTTACGACAAAACATTAAACGTAATCTGAATAACAAGGGACAGAAAACTAAGTTAAAGACCAACGTGAAGAATTTTCACAAGGAAATTAACCTAGACAACCTTGGTAATGTTTATTCCCAAGCAGATCGCTTAGCACGTAAAGGTATTATTTCTACTAATCGTGCGCGTCGTTTAAAATCACGCAACGCTGCTGTTTTAAATAAAACACAAGTAACAGCTGTTGAAGGCAAATAA", "aa": "MANIKSNEKRLRQNIKRNLNNKGQKTKLKTNVKNFHKEINLDNLGNVYSQADRLARKGIISTNRARRLKSRNAAVLNKTQVTAVEGK", "aa_hexdigest": "356901d664cb93989653b6191ea76d9f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386BED", "ncbi_nrp_id": "WP_014325593.1", "uniref100_id": "UniRef100_A0AAX0S2C1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386BED", "RefSeq:WP_014325593.1", "UniRef:UniRef100_A0AAX0S2C1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0S2C1", "uniref90_id": "UniRef90_P75237" }, "psc": { "uniref90_id": "UniRef90_P75237", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_P55750", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P55750", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55750" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "GIHJHMOALN_1157", "locus": "GCA009939745_01156" }, { "type": "cds", "sequence": "contig_1", "start": 655907, "stop": 656065, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTGGTGGACAACAGGGTGGTTTTTTTGGCCTACTCGTCATAATTATCCCTGTCATATTGTTGATTGTCTTTTTCTCTAAAAAGAAGAATTCACAAAAAACAGAGTTTGGTGGTGAAGGGGGCAGCCGTGCTTCCAAAAAAGATGAAGTTTGA", "aa": "MNGGGQQGGFFGLLVIIIPVILLIVFFSKKKNSQKTEFGGEGGSRASKKDEV", "aa_hexdigest": "b3b6d63816216fe6ea79769fefa8c627", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5528.383600000002, "isoelectric_point": 9.69799518585205 }, "id": "GIHJHMOALN_1158", "locus": "GCA009939745_01157" }, { "type": "cds", "sequence": "contig_1", "start": 656150, "stop": 656563, "strand": "+", "frame": 2, "gene": null, "product": "putative MG364-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604", "UniRef:UniRef90_P75236" ], "nt": "TTGCACTCCAGGAAAGACCGGCAGTTTTTTAACCAAGAAGTGCAAGCTTACATTACAGCCCATAACTTAAGCAAAACAGCAGCCAAGCGTTACCGTCACGAGCAATTAAAACTCAAACAACGTGAACTGTACTGCATTTACTTCATTACCAAGGACGCTAAAACCAGCGTTTTTGATGAAGCACGCATTATTGAAGCTGAAGTTTACCAAAAGCCCAATAAAACGGGTAAGGGTGCACCAGAACGTTTAATTCGAATCTTAGGATTGAAAAACTTCAATGACGAAATGAAGTGGATTCAACCACTAATGGATCGCGAGGAAAAACGAAAGGAAAAAGAAGAGAAACGCAAGCGGGAATTGGCAGCACGTCAGTTAAAAAGACAAGAAAAGAAAAAACAAAAAACTTCCAAATAA", "aa": "MHSRKDRQFFNQEVQAYITAHNLSKTAAKRYRHEQLKLKQRELYCIYFITKDAKTSVFDEARIIEAEVYQKPNKTGKGAPERLIRILGLKNFNDEMKWIQPLMDREEKRKEKEEKRKRELAARQLKRQEKKKQKTSK", "aa_hexdigest": "be6a29ee600b559f269b992cea5c13c5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 656006, "stop": 656563, "observations": { "mutation": [ 656063 ], "stop": [ 656063 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75236", "query_cov": 1.0, "subject_cov": 0.6284403669724771, "identity": 0.993, "score": 233.0, "evalue": 9.5e-76, "gene_start": 1, "gene_end": 137, "reference_start": 82, "reference_end": 218, "reference_sequence": "MNGGGQQGGFFGLLVIIIPVILLIVFFSKKKNSQKTEFGGEGGSRASKKDEVWKTVKQFLQEQNERGKEIIKTFVAKNPNPLHSRKDRQFFNQEVQAYITAHNLSKTAAKRYRHEQLKLKQRELYCIYFITKDAKTSVFDEARIIEAEVYQKPNKTGKGAPERLIRILGLKNFNDEMKWIQPLMDREEKRKEKEEKRKRELAARQLKRQEKKKQKTSK" }, "paralog": false, "identity": 0.9837837837837838, "score": 327.0, "evalue": 2.85e-115, "description": "Internal stop codon at 656063. Nonsense mutation around 656063.", "psc": { "uniref90_id": "UniRef90_P75236", "product": "Uncharacterized protein MG364 homolog", "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75236", "UniRef:UniRef50_P47604" ] }, "pscc": { "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604" ], "product": "Uncharacterized protein MG364" } }, "id": "GIHJHMOALN_1159", "locus": "GCA009939745_01158" }, { "type": "cds", "sequence": "contig_1", "start": 656568, "stop": 656933, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00551.24" ], "nt": "ATGATTAAAGTGGTTTTCTTTGGGACATCCACCCTTTCAAAGTGTTGTTTAGAAGCAATTTTTCAAGATCCTGAGTTTGTGGTTTGCGGCGTAGTGACCCAGCCCGATAAGGTAAACGAGCGCAACAATAAAATTAACTTTTCTGCAGTTAAACAATTTTGTATAGAAAACAACATTCCCTGTTTTCAACCAGAAAAAAACATTCAGATTAAAACAGAGTTAGCCCAACTCCAAGCTGATATTGGCGTTTGTGTAGCCTTTGGCCAATACATCCACAACGATATTATTAACCTCTTTCCTTACAAGATTGCCAACCTACACCCCTCAAAATTACCACTACTGCGTGGTGGAGCACCATTGCACTGA", "aa": "MIKVVFFGTSTLSKCCLEAIFQDPEFVVCGVVTQPDKVNERNNKINFSAVKQFCIENNIPCFQPEKNIQIKTELAQLQADIGVCVAFGQYIHNDIINLFPYKIANLHPSKLPLLRGGAPLH", "aa_hexdigest": "384d25ffad9edeb7870b72daad1e208b", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00551.24", "name": "Formyl_trans_N", "length": 123, "aa_cov": 0.9752066115702479, "hmm_cov": 0.975609756097561, "evalue": 6.698421787643934e-16, "score": 54.031036376953125, "start": 3, "stop": 120 } ], "seq_stats": { "molecular_weight": 13547.68919999998, "isoelectric_point": 7.602692604064942 }, "id": "GIHJHMOALN_1160", "locus": "GCA009939745_01159" }, { "type": "cds", "sequence": "contig_1", "start": 657321, "stop": 657503, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02911.23" ], "nt": "ATGACAAGTACAACCGCAGTTGGTCAAATCACTAAAATTTCTAAACAAGGTATAGAAATCGCCTTAGCTAATGATGAAATTGTTCTACTCCAAATAATTCAAATTCCGGGAAAGCGGGCAATGGGAGTGTCAGAAATAATTAACGGTAAACATCCTTTTGCTGAAGGTAAATTTTTTCATTAA", "aa": "MTSTTAVGQITKISKQGIEIALANDEIVLLQIIQIPGKRAMGVSEIINGKHPFAEGKFFH", "aa_hexdigest": "19dbfaca56bd86a3e6a12aada593cd65", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02911.23", "name": "Formyl_trans_C", "length": 44, "aa_cov": 0.7333333333333333, "hmm_cov": 0.9772727272727273, "evalue": 3.4973340790946125e-08, "score": 29.089759826660156, "start": 7, "stop": 50 } ], "seq_stats": { "molecular_weight": 6491.536899999999, "isoelectric_point": 8.18195667266846 }, "id": "GIHJHMOALN_1161", "locus": "GCA009939745_01160" }, { "type": "cds", "sequence": "contig_1", "start": 657503, "stop": 658627, "strand": "+", "frame": 2, "gene": null, "product": "putative MG366-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47606", "UniRef:UniRef50_UPI00031D7E73", "UniRef:UniRef90_P75234" ], "nt": "ATGCTCACCAAGGCCTTTTTTTTAAAAAACTTTGATCGCTCAAAGGAGTTGATTCCTCTGTCTTACGCTGATGTTTTTGGCGCTGCTAGTCAATTGTTAAAAAAACACCATAAGCAGGTCGATACCGAAATTGATGTGCAAGAAGATCTAATTGAAGATCCGGTATTTGAAATAGATATTTTAGAGCTACTCAACGAAGCGCCAGAACTATTATTTGATAGTAAAAATCCTCGGGTTAAAGAGGCACAAATCATTATTGAAAAGGCCAAAAAAGATATTGCTAGCTATTTCCACTTAGACAATATTTTAGACACTGACAACTTAGGGTTAAAAGCCACTGTTACCGAAAAAATCCAATTTACCGAAAAACAAATCGAAGCTGCAGTACAAAACAAAAAAGCAGCGATTATTTTTAAACCAGTGTTTACCGTCAACCAGTGTTTAATCCAACCAGATGCTGTAGTAGTACATGCTAATGGTTTGTGTGAGTTTGTTGTTATTAAAGCGACAACAAATACCAAGAGAAAATTTATCTTGGAAATTATTTATGACTTCTTGCTGTTTGAAAAATTGGGCAAATACAAACTGGTTAACTATTACTTCTGCATAGTTAATTATGAACTGAAAAATAAACACAATGTTTCCTTCTTTTTAAACACCGAAATTAAAACAGCCAAAAACAGTAGTACGAGTAAAACTAAAGAAGAGCAAGTGTTGTACGGGCATCTCCCTTTTAATGATCCAAAAAAAATTGCTTACATTCATAGCAAGAAGAGTGGTGGTGTCAATGGTTTTCTGCTCGTCAAACTAGTGGACAACATTATTAGAAGCGGTGTTACTAATTTGGAACAAATTATTAGTTTTGTTTTTCGTGAACTGAATGCACCCAGCATTCGTAGCTTAAAGCAAATTATTAGTGAAGTAGCTAAAGTACAACTTGATTTTTGGAACATTATTGATGATGTTAAACAACACCAAGAGTTACAAGACAACCAAATTACCTTCAATTACAGTGATGCCTTCAACTCTTTTTGGAATAACTATTTATTGCGCAATCTAATTAAATTAGTCTTTGCTTATAAATACAGTGAAATCTTTCGACTTTCAGGAAAATTAGCTAAATGA", "aa": "MLTKAFFLKNFDRSKELIPLSYADVFGAASQLLKKHHKQVDTEIDVQEDLIEDPVFEIDILELLNEAPELLFDSKNPRVKEAQIIIEKAKKDIASYFHLDNILDTDNLGLKATVTEKIQFTEKQIEAAVQNKKAAIIFKPVFTVNQCLIQPDAVVVHANGLCEFVVIKATTNTKRKFILEIIYDFLLFEKLGKYKLVNYYFCIVNYELKNKHNVSFFLNTEIKTAKNSSTSKTKEEQVLYGHLPFNDPKKIAYIHSKKSGGVNGFLLVKLVDNIIRSGVTNLEQIISFVFRELNAPSIRSLKQIISEVAKVQLDFWNIIDDVKQHQELQDNQITFNYSDAFNSFWNNYLLRNLIKLVFAYKYSEIFRLSGKLAK", "aa_hexdigest": "d7297195fa447fccef86a89f0f9c2dd4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00031D7E73", "query_cov": 0.9973262032085561, "subject_cov": 0.8197802197802198, "identity": 0.627, "score": 470.0, "evalue": 7.74e-162, "valid": false, "uniref50_id": "UniRef50_UPI00031D7E73" }, "pscc": { "uniref50_id": "UniRef50_UPI00031D7E73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00031D7E73" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 657503, "stop": 658930, "observations": { "mutation": [ 658625, 658769, 658817 ], "stop": [ 658625, 658769, 658817 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75234", "query_cov": 1.0, "subject_cov": 0.5632530120481928, "identity": 1.0, "score": 721.0, "evalue": 1.44e-257, "gene_start": 1, "gene_end": 374, "reference_start": 1, "reference_end": 374, "reference_sequence": "MLTKAFFLKNFDRSKELIPLSYADVFGAASQLLKKHHKQVDTEIDVQEDLIEDPVFEIDILELLNEAPELLFDSKNPRVKEAQIIIEKAKKDIASYFHLDNILDTDNLGLKATVTEKIQFTEKQIEAAVQNKKAAIIFKPVFTVNQCLIQPDAVVVHANGLCEFVVIKATTNTKRKFILEIIYDFLLFEKLGKYKLVNYYFCIVNYELKNKHNVSFFLNTEIKTAKNSSTSKTKEEQVLYGHLPFNDPKKIAYIHSKKSGGVNGFLLVKLVDNIIRSGVTNLEQIISFVFRELNAPSIRSLKQIISEVAKVQLDFWNIIDDVKQHQELQDNQITFNYSDAFNSFWNNYLLRNLIKLVFAYKYSEIFRLSGKLAKWDEVVEAYKENKSVKIDGFLYELNQGKMKKTKNPATSQFNKIHFFLRAWNDKKGIAVGNKFKSVWQKLKEKKVYFDFETISSAVRVIDKSLPFTQIVTQCSLIVDDNTESDKSKLVCQNLIFDPLTIGIEDFKTVVDALYQKQCDQYSFVVYNKSFEKNRLLEMATFINEAPYQQRVQAIIENLFDLADIFGLENDCLAFKQLDGFSSIKKVLPMIDQRFLDASRTVSYQSLKVQKGDVAQELTLARFLNCLDEQQWAQTALELKQYCENDVRAMIAIELFIKDFITNQL" }, "paralog": false, "identity": 0.991578947368421, "score": 920.0, "evalue": 0.0, "description": "Internal stop codon at 658625, 658769, 658817. Nonsense mutation around 658625, 658769, 658817.", "psc": { "uniref90_id": "UniRef90_P75234", "product": "Uncharacterized protein MG366 homolog", "uniref50_id": "UniRef50_P47606", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75234", "UniRef:UniRef50_P47606" ] }, "pscc": { "uniref50_id": "UniRef50_P47606", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47606" ], "product": "Uncharacterized protein MG366" } }, "id": "GIHJHMOALN_1162", "locus": "GCA009939745_01161" }, { "type": "cds", "sequence": "contig_1", "start": 658913, "stop": 659395, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "GTGACCCAGTGTTCTCTTATTGTTGATGACAATACCGAGTCTGATAAAAGCAAGTTAGTTTGTCAAAACCTGATCTTTGATCCGCTAACTATTGGTATTGAGGACTTTAAAACAGTCGTTGATGTCCTTTACCAAAAGCAGTGTGACCAGTACAGTTTTGTCGTTTATAACAAATCGTTTGAAAAGAACAGGTTACTGGAAATGGCTACTTTTATCAATGAAGCTCCATACCAACAAAGAGTGCAAGCAATTATTGAAAATTTATTTGATTTAGCAGATATTTTTGGACTTGAAAATGATTGCTTAGCCTTTAAACAACTTGATGGTTTTTCATCAATTAAAAAGGTCTTGCCAATGATTGATCAACGCTTTTTAGATGCTAGTCGAACTGTTAGTTACCAATCATTAAAGGTACAAAAAGGTGATGTAGCACAAGAGCTTACTTTGGCACGCTTTTTAAATTGTTTAGACGAACAGCAATGA", "aa": "MTQCSLIVDDNTESDKSKLVCQNLIFDPLTIGIEDFKTVVDVLYQKQCDQYSFVVYNKSFEKNRLLEMATFINEAPYQQRVQAIIENLFDLADIFGLENDCLAFKQLDGFSSIKKVLPMIDQRFLDASRTVSYQSLKVQKGDVAQELTLARFLNCLDEQQ", "aa_hexdigest": "542f8fc604fa8451e9aa5057cf4fe7cd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 112, "aa_cov": 0.69375, "hmm_cov": 0.9017857142857143, "evalue": 1.2597412947665104e-14, "score": 50.1009635925293, "start": 2, "stop": 112 } ], "seq_stats": { "molecular_weight": 18413.83189999999, "isoelectric_point": 4.52236042022705 }, "id": "GIHJHMOALN_1163", "locus": "GCA009939745_01162" }, { "type": "cds", "sequence": "contig_1", "start": 659538, "stop": 660158, "strand": "-", "frame": 2, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "COG:COG0571", "COG:K", "EC:3.1.26.3", "GO:0003723", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47607", "UniRef:UniRef90_P75233" ], "nt": "TTGGAATTTTTAGGTGATGCCTTAATTGATTTCATAGTAGCGAAAAAACTGTTTGAGCTTTATCCCAATTACAACGAAGGTATGTTGACCAGAACTAAGATTGAAATTGTTAAGGGCGAAAACCTCAACCGTATTGGGAAGGAATTGAACTTTGGCAATTTCATTAAGTTAGGTAAAGGAATGCCGTATACTGAAACACTTTTTGGTGATGTACTAGAGGCGTTAGTGGCAGCCATTTACGAAGACCTTGGCATAGAGAAAGCGAATCAATTTGTCGAAGAACATATTTTTAAAAAAACTTACTCAGAAATTCTCAAGTACAACTTCTTTTCACTGTTTCAAGAACAAAAGCTACCAGAACCTAGGGTACGGGTTAGTTTAACTAGCAATAACCTTGTGTTGAGTATTATTGAGCTTAACGGTGACATTATCTGGTCACAAGCTGTACCTAACAGTAAACACTATGATGACAAGAGCGTGTTAGAACACAATGCAATGTCAGCCTTTACCCAGTTTCTTAAAAGTGGTAAAGGCATTAACTTTTTTAGTGACATTAAAAATAAGTTAGACAGTCAAAAGCCTATGCGAGCTTTAACAGTTAGACCGAAAAAGATTAACTGA", "aa": "MEFLGDALIDFIVAKKLFELYPNYNEGMLTRTKIEIVKGENLNRIGKELNFGNFIKLGKGMPYTETLFGDVLEALVAAIYEDLGIEKANQFVEEHIFKKTYSEILKYNFFSLFQEQKLPEPRVRVSLTSNNLVLSIIELNGDIIWSQAVPNSKHYDDKSVLEHNAMSAFTQFLKSGKGINFFSDIKNKLDSQKPMRALTVRPKKIN", "aa_hexdigest": "737f0a18fe7873f149c09b795d6154a2", "start_type": "TTG", "rbs_motif": null, "genes": [ "rnc" ], "truncated": "both", "pseudogene": { "start": 659481, "stop": 660329, "observations": { "mutation": [ 659540, 660236 ], "stop": [ 659540, 660236 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75233", "query_cov": 1.0, "subject_cov": 0.7304964539007093, "identity": 0.995, "score": 407.0, "evalue": 1.52e-142, "gene_start": 1, "gene_end": 206, "reference_start": 58, "reference_end": 263, "reference_sequence": "MKNKKDKTQKPKVIDEKFVAFFKSLNIEPQNWQFYEDAFVHSSYVNENEDARASYDRLEFLGDALIDFIVAKKLFELYPNYNEGMLTRTKIEIVKGENLNRIGKELNFGNFIKLGKGMPYTETLFGDVLEALVAAIYEDLGIEKANQFVEEHIFKKTYSEILKYNFFSLFQEQKLPEPRVRVSLTSNNLVLSIIELNGDIIWSQAVPNSKHYDDKSVLEHNAMSAFTQFLKSGKGINFFSDIKNKLDSQKPMRALTVRPKKINWKARKPKLKALKNKVKADS" }, "paralog": false, "identity": 0.9929078014184397, "score": 552.0, "evalue": 4.03e-202, "description": "Internal stop codon at 659540, 660236. Nonsense mutation around 659540, 660236.", "psc": { "uniref90_id": "UniRef90_P75233", "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_P47607", "cog_id": "COG0571", "cog_category": "K", "go_ids": [ "GO:0003723", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75233", "EC:3.1.26.3", "UniRef:UniRef50_P47607", "COG:COG0571", "COG:K", "GO:0003723", "GO:0004525", "GO:0005737", "GO:0006364", "GO:0006396", "GO:0006397", "GO:0008033", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47607" ], "product": "Ribonuclease 3" } }, "id": "GIHJHMOALN_1164", "locus": "GCA009939745_01163" }, { "type": "cds", "sequence": "contig_1", "start": 660234, "stop": 660329, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAGAAAGATAAAACTCAAAAACCTAAGGTCATTGATGAAAAGTTTGTCGCTTTTTTCAAGAGTTTAAACATTGAACCGCAAAACTGA", "aa": "MKNKKDKTQKPKVIDEKFVAFFKSLNIEPQN", "aa_hexdigest": "611bbaf44cf0d061be9744ba31977735", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3694.304400000001, "isoelectric_point": 9.778516197204588 }, "id": "GIHJHMOALN_1165", "locus": "GCA009939745_01164" }, { "type": "cds", "sequence": "contig_1", "start": 660319, "stop": 661305, "strand": "-", "frame": 1, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811", "KEGG:K03621", "RefSeq:WP_014325596.1", "SO:0001217", "UniParc:UPI0001DD642C", "UniRef:UniRef100_A0AAN4XGJ0", "UniRef:UniRef50_Q49427", "UniRef:UniRef90_P75232" ], "nt": "ATGGCTTTTAGACTAGCAGTTGATTGTTTAGGTTTTGAAAACCACCCGCGTGAAGCAATTGACGCAGTATTAGAATACTGGAGCTATCATCAAGAGTTGGAATTTATCTTAGTTGGTGATGAAAAGACCTTTGACGGACTTGATTATCTACCTAAAAACATTACCAAGCAATTAGCCAGCTCTTGCATAGATATGACCGACACACCACTAACCGCACGGCGCAAGGTTAACAACTCAATGCAGAAAGCGATTAACCTTGTGCGTGATGGTGCTGCTGATGTTGTGATTTCCGCTGGCTCCTCGGCCGTTTATGCCTCTTTAACATACGATGGCTTTGGCAAAATCCATAAAGATGTTAAAAGTGCTTTTATGTCTTATGTACCAACTGCCAATAACGACTGGTTTTACTTTTTAGATGTTGGCGCTAACAAGAACTTTACGGGTAAAGAGCTCTATTTTTTGGGCCTAATGGCGGATATTTTTGTCAAAAAGACCACCAACAAAATTAGTCCACGTATAGCATTGCTAAACATTGGTACCGAAATCAATAAAGGTTTTGATTACCACCAGGAAGGCTACCAATTGCTAAACGAAGATAAGCACCTTAACTTCACTGGTTTCATTGAACCACGCTTCTTACTTGACGGGGTGTGTGATATCTTAGTGGCTGATGGTTACAGCGGTAATCTTGTGTTGAAATCAATGGAAGGCACCTTTAAAACAATTGCGCGTCTTCTCAAGCAAGGTTATAAGCGTAACCCGTTGGCTGGGCTTTTTAGTTTGGGAATTTTGAAAAGAATTGCTAAGCGTTTTGATTACAAAAATAACGCAGGCGCCGTTGTTATCGGTTTAAATAAACTTGCTTTAAAAACCCATGGATCAGCTGATAAACAACAGTTTTTAAGCACCATTAGATTAGCACACACTAGCTTAAAGTCTGATCTAATTAACGCCATTAAAAGTTCCTTAGATAACTATGAAAAATAA", "aa": "MAFRLAVDCLGFENHPREAIDAVLEYWSYHQELEFILVGDEKTFDGLDYLPKNITKQLASSCIDMTDTPLTARRKVNNSMQKAINLVRDGAADVVISAGSSAVYASLTYDGFGKIHKDVKSAFMSYVPTANNDWFYFLDVGANKNFTGKELYFLGLMADIFVKKTTNKISPRIALLNIGTEINKGFDYHQEGYQLLNEDKHLNFTGFIEPRFLLDGVCDILVADGYSGNLVLKSMEGTFKTIARLLKQGYKRNPLAGLFSLGILKRIAKRFDYKNNAGAVVIGLNKLALKTHGSADKQQFLSTIRLAHTSLKSDLINAIKSSLDNYEK", "aa_hexdigest": "0a52ba0fd5334dfc6f9b4806153468a7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD642C", "ncbi_nrp_id": "WP_014325596.1", "uniref100_id": "UniRef100_A0AAN4XGJ0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD642C", "RefSeq:WP_014325596.1", "UniRef:UniRef100_A0AAN4XGJ0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAN4XGJ0", "uniref90_id": "UniRef90_P75232" }, "psc": { "uniref90_id": "UniRef90_P75232", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q49427", "cog_id": "COG0416", "cog_category": "I", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q49427", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49427" ], "product": "Phosphate acyltransferase" }, "genes": [ "plsX" ], "id": "GIHJHMOALN_1166", "locus": "GCA009939745_01165" }, { "type": "cds", "sequence": "contig_1", "start": 661305, "stop": 662444, "strand": "-", "frame": 2, "gene": "fakA", "product": "putative MG369-like protein", "db_xrefs": [ "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071", "SO:0001217", "UniRef:UniRef50_P75231", "UniRef:UniRef90_P75231" ], "nt": "ATGCTCCCAGTGTTAAAGGCCTCTGGTGTAGTAGATTCAGGTGCTTATGGGTTCGCTTGCTTTTTAGAGGGGATGTTGTCCTTTTATGGTGAAAAGGCTACTTTAAACGATGGTAAGCTCACCAGTGCTGAATTGAGCCAAATGACCATTAGCGGTGAAAAACACGTTACGGAAGAAGAGTTTGGTTACTGCACTGAATATGTCTTGAAACTGGGCATGTCAGTAAGTCAAGAAGTTGAAAAGCAGAAGTTTAACCAAAAGAAGTTTGAAAGTAAGGTGAGCAAAATTGCCACTTCGGTAGTAGTTGCTAGTGATAAAGATAACGGCTTTGTTAAAGTACACGCCCATACCGAAAAACCTAACCTGTTATTAGAACTTGGGCTTAACTATGGTGAGTTTGAACTCGTCAAAATTGAAAACATGAATTTACAGGTAGCGAAGCAAAAACCCGCTCCTGTGAAGCGCAACATTAAACCAGCCATAGTAGTGACTGTACCAACTGAAGCTTTTGCTGATCGGATTCGCGAAGACTATGACATTCAAGCTATCTTGTGTACCGATGACACCGGTGCACCTTCGGTCTTTTCGTTACTAGAAGCAGTCAAGCTAACTCATTCCAGTAACATTATTTTCTTGTTACATGACAAGAACTACTTTCTCTCTGCCAACGAAGCGTTAAAACAATTAAAGCACCAAAAGATTAGTGCTGATTGTGTGATGACTACCAACCCGATTGAATCACTAGCGGCGCTTACTGTTTTTAACAGTGACCTTAACATTCACACCAATGTCAAAACAATGCGCCGTTTTGTCAAAGGCTTTGCATCTGCAACCATTACTCAAGCTTCGAAAAAGTACAAGGAAAATCGGATTGAAGTTAATAAGGGTGACTTTATTGCTGTAGCTAATAACAGCATTTGTGTTAGTGAAAAAGAGCTTGTTCAATGCGTGTTCAACACCATTGACCACCTTTTGAAAAAGGTAAAAAAGCCGGAGTTTCTTTTAGCTTACTATGGTAAAGATATCACCGCTGAGGAAGCGGAAGCAATGAAGGAAAAAATAGAAAAGAAATACAAGCTTTTCTGTGAGTTTTCCCCAGGGGAACAAAAAGTTTTTTCTTATATACTAGGCATACAATAA", "aa": "MLPVLKASGVVDSGAYGFACFLEGMLSFYGEKATLNDGKLTSAELSQMTISGEKHVTEEEFGYCTEYVLKLGMSVSQEVEKQKFNQKKFESKVSKIATSVVVASDKDNGFVKVHAHTEKPNLLLELGLNYGEFELVKIENMNLQVAKQKPAPVKRNIKPAIVVTVPTEAFADRIREDYDIQAILCTDDTGAPSVFSLLEAVKLTHSSNIIFLLHDKNYFLSANEALKQLKHQKISADCVMTTNPIESLAALTVFNSDLNIHTNVKTMRRFVKGFASATITQASKKYKENRIEVNKGDFIAVANNSICVSEKELVQCVFNTIDHLLKKVKKPEFLLAYYGKDITAEEAEAMKEKIEKKYKLFCEFSPGEQKVFSYILGIQ", "aa_hexdigest": "1305cb2248b8843c5d3ec1e68f7fc9ab", "start_type": "ATG", "rbs_motif": null, "genes": [ "fakA" ], "truncated": "3-prime", "pseudogene": { "start": 661305, "stop": 662744, "observations": { "mutation": [ 662471 ], "stop": [ 662471 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75231", "query_cov": 1.0, "subject_cov": 0.6792114695340502, "identity": 1.0, "score": 732.0, "evalue": 1.09e-263, "gene_start": 1, "gene_end": 379, "reference_start": 180, "reference_end": 558, "reference_sequence": "MSTVNLSNFIDMLRLGCKNIANNFEYINQLNVFPVPDGDTGTNMKVTLSEAFKKLESEISHIKSFSDLGKSFTRDLLLFSRGNSGVIFSQIMKGFFSDMISTKTATETELGIEDFATAFIKAEEVAYKNVSKPVEGTMLTVIRLISTDFKNQKNRAKTVQKLFEQVIKTAWQTVKKTPQMLPVLKASGVVDSGAYGFACFLEGMLSFYGEKATLNDGKLTSAELSQMTISGEKHVTEEEFGYCTEYVLKLGMSVSQEVEKQKFNQKKFESKVSKIATSVVVASDKDNGFVKVHAHTEKPNLLLELGLNYGEFELVKIENMNLQVAKQKPAPVKRNIKPAIVVTVPTEAFADRIREDYDIQAILCTDDTGAPSVFSLLEAVKLTHSSNIIFLLHDKNYFLSANEALKQLKHQKISADCVMTTNPIESLAALTVFNSDLNIHTNVKTMRRFVKGFASATITQASKKYKENRIEVNKGDFIAVANNSICVSEKELVQCVFNTIDHLLKKVKKPEFLLAYYGKDITAEEAEAMKEKIEKKYKLFCEFSPGEQKVFSYILGIQ" }, "paralog": false, "identity": 0.9979123173277662, "score": 929.0, "evalue": 0.0, "description": "Internal stop codon at 662471. Nonsense mutation around 662471.", "psc": { "uniref90_id": "UniRef90_P75231", "gene": "FakA", "product": "Uncharacterized protein MG369 homolog", "uniref50_id": "UniRef50_P75231", "cog_id": "COG1461", "cog_category": "I", "go_ids": [ "GO:0004371", "GO:0006071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75231", "UniRef:UniRef50_P75231", "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071" ] }, "pscc": { "uniref50_id": "UniRef50_P75231", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75231" ], "product": "Uncharacterized protein MG369 homolog" } }, "id": "GIHJHMOALN_1167", "locus": "GCA009939745_01166" }, { "type": "cds", "sequence": "contig_1", "start": 662469, "stop": 662981, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTACAGTAAATTTAAGCAACTTTATTGACATGTTGCGTCTTGGTTGTAAGAATATAGCTAATAATTTCGAATACATTAATCAACTCAATGTTTTCCCGGTGCCAGATGGTGATACGGGGACAAACATGAAGGTCACGTTGAGTGAAGCGTTCAAAAAACTAGAGAGTGAAATTAGCCACATTAAGAGCTTTAGTGATTTGGGCAAAAGCTTTACCCGTGACCTTTTACTTTTCTCACGCGGTAACTCTGGTGTTATTTTTTCCCAAATTATGAAGGGCTTTTTTAGTGACATGATTAGCACTAAAACAGCCACCGAAACCGAACTTGGCATAGAGGATTTTGCTACAGCTTTTATAAAGGCCGAAGAGGTGGCATACAAGAATGTCTCCAAGCCCGTAGAGGGTACGATGCTCACGGTTATTCGCTTAATTAGCACAGACTTTAAAAACCAAAAGAACCGTGCCAAAACAGTGCAAAAACTGTTTGAACAAGTAATTAAAACAGCCTGA", "aa": "MSTVNLSNFIDMLRLGCKNIANNFEYINQLNVFPVPDGDTGTNMKVTLSEAFKKLESEISHIKSFSDLGKSFTRDLLLFSRGNSGVIFSQIMKGFFSDMISTKTATETELGIEDFATAFIKAEEVAYKNVSKPVEGTMLTVIRLISTDFKNQKNRAKTVQKLFEQVIKTA", "aa_hexdigest": "52f3e1424588af7cb74f9eee66caf169", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19027.6914, "isoelectric_point": 8.62466106414795 }, "id": "GIHJHMOALN_1168", "locus": "GCA009939745_01167" }, { "type": "cds", "sequence": "contig_1", "start": 663069, "stop": 663404, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAACAGTTAAGGCCAAACCATTTCCTAACGCTAAACCGATTACCACCATTTTTGAAAACAGTAAGTACTTAACTAAGTTAGATCTTTCGTTGCTTACTATACGCTTAGTAAGTGGTAAAACCCACCAAATTCGTGCTTGCTTAAACCTCTTGAATACCCAGTTAGTTGGTGATAGAAAATACCAATTACTGCAATTTAAAAACCGTAACCGCGATTTTAAACACCAAGCCTTACATGCCACAAATCTAAGTTTTGCCCAATTAGATAAGCAGAAATTTCCCTTACTGGCTAATTACTCGCAACGGCAGTTTAAAAGCCAACTAGTACCGTGA", "aa": "MVTVKAKPFPNAKPITTIFENSKYLTKLDLSLLTIRLVSGKTHQIRACLNLLNTQLVGDRKYQLLQFKNRNRDFKHQALHATNLSFAQLDKQKFPLLANYSQRQFKSQLVP", "aa_hexdigest": "c96dc94f449ef1bc5722ce1f013a8783", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12855.907599999984, "isoelectric_point": 10.4221040725708 }, "id": "GIHJHMOALN_1169", "locus": "GCA009939745_01168" }, { "type": "cds", "sequence": "contig_1", "start": 663426, "stop": 664016, "strand": "-", "frame": 2, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG370-like protein", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0120159", "SO:0001217", "UniRef:UniRef50_P47610", "UniRef:UniRef90_P75230" ], "nt": "ATGTCAACAGCTAAGTTTGTAGTGCCTAAAGCTGTTGAAAACGTTAGGATTGAAAAGTTTTGTTTAAAGTTGTTGCCCAACATTAAGCTAAGTCAGTTCTTTAAATTGTTGCGCTTAGGTAAGGTCTTGCTCAATAACACGAAGGCTAAATTAGGTCAGCGTGTTAGCAGCGGTGATACAATCGTTTTTCAGTTTGCAATTGAACCTTACTTACACAACCACAGCGAAGTAGTGGATTTAACCCAAGTCCAAGATGATTTGCAGGTAATCTTTGAGGATGAACAGCTCATTGTGGTGGACAAACCAGCGGGTGTAGTGTGTCAACCCGATGCTAAACACGAACTGTTTAATTTAGCTAACAGCTTACTGAAACACTGTGGTTATAATCAATACTTTAAAAACAGTCTTAGCTTCATACCACGGTTTGCGCACCGAATTGATCGTAATACCTGTGGTTTGGTAATAGGTGCTAAAACCAACCAGGCATTACAGGAGTTAGAGGCGGTTTTTCGCCATAACTTGTTAATGAAACAATACCAAGGATTAGTGTTTGGCCCATTTAATTTTAAGGGCACACAAAAAGCTTATTGA", "aa": "MSTAKFVVPKAVENVRIEKFCLKLLPNIKLSQFFKLLRLGKVLLNNTKAKLGQRVSSGDTIVFQFAIEPYLHNHSEVVDLTQVQDDLQVIFEDEQLIVVDKPAGVVCQPDAKHELFNLANSLLKHCGYNQYFKNSLSFIPRFAHRIDRNTCGLVIGAKTNQALQELEAVFRHNLLMKQYQGLVFGPFNFKGTQKAY", "aa_hexdigest": "6e8b5124e74d0f1360d9faa9fdf7b300", "start_type": "ATG", "rbs_motif": null, "genes": [ "rluA" ], "truncated": "5-prime", "pseudogene": { "start": 663123, "stop": 664016, "observations": { "mutation": [ 663428 ], "stop": [ 663428 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75230", "query_cov": 1.0, "subject_cov": 0.6012269938650306, "identity": 1.0, "score": 390.0, "evalue": 1.67e-135, "gene_start": 1, "gene_end": 196, "reference_start": 1, "reference_end": 196, "reference_sequence": "MSTAKFVVPKAVENVRIEKFCLKLLPNIKLSQFFKLLRLGKVLLNNTKAKLGQRVSSGDTIVFQFAIEPYLHNHSEVVDLTQVQDDLQVIFEDEQLIVVDKPAGVVCQPDAKHELFNLANSLLKHCGYNQYFKNSLSFIPRFAHRIDRNTCGLVIGAKTNQALQELEAVFRHNLLMKQYQGLVFGPFNFKGTQKAYWAKDAYQALVTVKAKPFPNAKPITTIFENSKYLTKLDLSLLTIRLVSGKTHQIRACLNLLNTQLVGDRKYQLLQFKNRNRDFKHQALHATNLSFAQLDKQKFPLLANYSQRQFKSQLVPWFASLIKGVSI" }, "paralog": false, "identity": 0.9966329966329966, "score": 588.0, "evalue": 1.37e-215, "description": "Internal stop codon at 663428. Nonsense mutation around 663428.", "psc": { "uniref90_id": "UniRef90_P75230", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG370 homolog", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47610", "cog_id": "COG0564", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0120159", "GO:0000455" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75230", "EC:5.4.99.-", "UniRef:UniRef50_P47610", "COG:COG0564", "COG:J", "GO:0003723", "GO:0120159", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47610", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47610" ], "product": "Uncharacterized RNA pseudouridine synthase MG370" } }, "id": "GIHJHMOALN_1170", "locus": "GCA009939745_01169" }, { "type": "cds", "sequence": "contig_1", "start": 664003, "stop": 664125, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTGGTGGTGGGCACAAGTATGCTGCTGCCTTTGTGTTGGATGACAAGCGTCAGTTTATGAAGTTAGTGGAAATTATGGATGACTTTTTAGCCAAACAAAAGCATGTCAACAGCTAA", "aa": "MFGGGGHKYAAAFVLDDKRQFMKLVEIMDDFLAKQKHVNS", "aa_hexdigest": "6ad50d8c0e8924b3ec1958914c3de93b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4544.239600000001, "isoelectric_point": 8.140052223205569 }, "id": "GIHJHMOALN_1171", "locus": "GCA009939745_01170" }, { "type": "cds", "sequence": "contig_1", "start": 664174, "stop": 664980, "strand": "-", "frame": 1, "gene": "nrnA", "product": "putative MG371-like protein", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.13.3", "EC:3.1.3.7", "GO:0003676", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_P75229", "UniRef:UniRef90_P75229" ], "nt": "ATGATTAACATCGATCCCCATTTTATTCATAACCTAACTAATAAACTCAAGACATTCGATAACTTTTCGTTATATGTGCATGTAAACCCGGACTTTGATGCCTTTGGCGCTGCTTTTGCCTTTAAGGCCTTTTTAGCGGTTTACTTCCCCCATAAAAAGGCTTACGTGATGGGATCACACAACATTAAGGCTGACGGGAAAGATTTGTTCCCCTTTGAAGCGGCACCTATTGATGATGCGTTTGTAAAAAACTCATTGGCAATTATCTTTGACACCTCCAACCAAGAACGAGTATTAACCCAAAAACATAAATTAGCTAAAGAAACCGTGCGGATTGATCACCATCCTAAAACCGAGAGTTTTGCTGATCTGGAATGGATTGACCCGGCTTTTTCAGCAGCAGCAGAAATGGTCGGTTACTTAATCTTGCAAATGGGTTATGAACTTAATGCTGAGATGGCTGCTTACATTTATGCGGGTATTATTACCGATACACAGCGCTTTTCCAGTTCCGCTACCACCCCACAAACCTTTGCTTTAACTGCTAAGTTACTAGAAACTGGATTTAACCGCAATAAGGTTCATGATGCAGTTTATCTAAAACCACTTTTAGAACACAAGTACTTTAGCTATGTCTTAAACAAAGCCAAGATCACCCCGAATGGTTTGGCTTACGCACTTCTTAAAAAAGGGACTTATAAGCAGTTTGGGGTGGTCTCACCACTACCGATGGTGCACGCTTTAAACAACATCAAAGGAGTCAAAATTTGGACGACTTGTTACTTTAACGAAGACATTAAGAAGTGA", "aa": "MINIDPHFIHNLTNKLKTFDNFSLYVHVNPDFDAFGAAFAFKAFLAVYFPHKKAYVMGSHNIKADGKDLFPFEAAPIDDAFVKNSLAIIFDTSNQERVLTQKHKLAKETVRIDHHPKTESFADLEWIDPAFSAAAEMVGYLILQMGYELNAEMAAYIYAGIITDTQRFSSSATTPQTFALTAKLLETGFNRNKVHDAVYLKPLLEHKYFSYVLNKAKITPNGLAYALLKKGTYKQFGVVSPLPMVHALNNIKGVKIWTTCYFNEDIKK", "aa_hexdigest": "7ecaa9433da3a58a934864d1378b3540", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75229", "query_cov": 1.0, "subject_cov": 0.8246153846153846, "identity": 1.0, "score": 538.0, "evalue": 9.88e-193, "valid": true, "gene": "NrnA", "product": "Uncharacterized protein MG371 homolog", "ec_ids": [ "3.1.13.3", "3.1.3.7" ], "uniref50_id": "UniRef50_P75229", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "go_ids": [ "GO:0003676" ] }, "pscc": { "uniref50_id": "UniRef50_P75229", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75229" ], "product": "Uncharacterized protein MG371 homolog" }, "genes": [ "nrnA" ], "id": "GIHJHMOALN_1172", "locus": "GCA009939745_01171" }, { "type": "cds", "sequence": "contig_1", "start": 664973, "stop": 665110, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACCTGTACTGTGTTTGCTCCCAAAAAACCAACTACTAAGGCTAAGTTAGCGATCGTTGAAAAGCTTGAGAGTGAACTTTTGTTTGTCCGTGAACTAATTGAAGCTGGTGTCAAGAAGCTGCAAAATGATTAA", "aa": "MDTCTVFAPKKPTTKAKLAIVEKLESELLFVRELIEAGVKKLQND", "aa_hexdigest": "b23bbd64bd2dfe4cacb12c9dbca1bfea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5046.941199999999, "isoelectric_point": 7.871863746643066 }, "id": "GIHJHMOALN_1173", "locus": "GCA009939745_01172" }, { "type": "cds", "sequence": "contig_1", "start": 665153, "stop": 666136, "strand": "-", "frame": 3, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "EC:2.8.1.4", "GO:0000049", "GO:0002937", "GO:0004810", "GO:0005524", "GO:0005829", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0140741", "KEGG:K03151", "SO:0001217", "UniRef:UniRef50_P47612", "UniRef:UniRef90_P75228" ], "nt": "ATGGGATTGCATAATGAACCAAATACTATTCTAATCTGCCGTTATGGCGAACTTGTTTTAAAGGGCAAGAACCGCTTGCAGTTTGTCAAACAACTCAAAAAGAACGTTAAACAAGCCTTTAAGAAACTAAGCATTACTAACCCAGTTGACTACCAGTTTGACATGTTGGTAGTGGGTGAAGTAATTTCCACCCAAAGATCGTTATTAAAAAATCTCTTTACCAGATTACCGGGTTTGAGTGTTTGCTTGTTTGCCTTACAAATCCCTCATGATGAAGCGCAATTGTTAGCACTGCTACAGCAAGTGGTACAATCCCACCCATCGTTCAAAATTGAGGTAAGACGGCGTGATAAATTATTTGCTTGCAATTCCAGTGCCTTCAAAAAGTACCTAGCTTTACAGTTATGGGAAAAATACCAATTGAAGGGTAAGTTAGTTGACCCTGCCATTACGGTGCATGTCGAAGTAACTAAGGAACATTTCCTAATAATTAGTGAAAGCTTTAATGGTATTGGTGGTCTACCGGTCTTTACTAGTGGTACTGCTTTGGCGTTACTGTCTGGTGGCATAGACAGTCCAGTAGCCGCTTCCCTAGTTTTACAACGAGGTTTTAATGTTGACTTTATTACTTTTATTAATGAGCCCGGACATAACGCTGCCACAATAGGCAAAATTCAACGGTTAGCTAACTTAGTTTCCCTTAACCAAACGCTTTGTACTGGGAGACTGTTTGTCTTTGACTTTACCGATTTACAGAAAGAGTTGAGTCATATTAGTCTGGAAGGATACCGGATTGTTTTAATGCGCCGTTGCTTTTACAAGATTGCTAGTTTGTTTAAATATGATTGTTTAATCACCGGGGAAGCATTGGGACAAGTAGCTTCACAAACAATTGATAATTTGAAGGTAATCCAAGCTGTTGTGCCCAATACCTTTGTGATTCGCCCGTTAATAGGCTTAAGTAAAGATAAAATAATTGAATGA", "aa": "MGLHNEPNTILICRYGELVLKGKNRLQFVKQLKKNVKQAFKKLSITNPVDYQFDMLVVGEVISTQRSLLKNLFTRLPGLSVCLFALQIPHDEAQLLALLQQVVQSHPSFKIEVRRRDKLFACNSSAFKKYLALQLWEKYQLKGKLVDPAITVHVEVTKEHFLIISESFNGIGGLPVFTSGTALALLSGGIDSPVAASLVLQRGFNVDFITFINEPGHNAATIGKIQRLANLVSLNQTLCTGRLFVFDFTDLQKELSHISLEGYRIVLMRRCFYKIASLFKYDCLITGEALGQVASQTIDNLKVIQAVVPNTFVIRPLIGLSKDKIIE", "aa_hexdigest": "88c17433a7a858e83239292eb5fef1b1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75228", "query_cov": 1.0, "subject_cov": 0.8449612403100775, "identity": 1.0, "score": 635.0, "evalue": 6e-229, "valid": true, "gene": "thiI", "product": "Probable tRNA sulfurtransferase", "ec_ids": [ "2.8.1.4" ], "uniref50_id": "UniRef50_P47612", "cog_id": "COG0301", "cog_category": "HJ", "kegg_orthology_id": "K03151", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0004810", "GO:0000049", "GO:0140741", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0002937" ] }, "pscc": { "uniref50_id": "UniRef50_P47612", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47612" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [ "thiI" ], "id": "GIHJHMOALN_1174", "locus": "GCA009939745_01173" }, { "type": "cds", "sequence": "contig_1", "start": 666147, "stop": 666794, "strand": "-", "frame": 2, "gene": null, "product": "MPN551 family DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227", "UniRef:UniRef90_P75227" ], "nt": "ATGGTCAAGATTTATGAGGATGAATTTGATGAAACACTCTCACGCGCGGGGCAAGTTATTGAACCCAAAATCCGTGATTATGTGATAGCTAAAACTGGTTTTAATTTCCATTCGTATGATCCCAAGGAGGTTAAGTGGGATCTCTTTCCTGAAAATCCAGTTTTTGGAGGAATTCCTGATGGGGAACCGGTCGATGTTTATGGAAAATTGGCTTACGATACCAATGCGCCGATGTTAGAAATTAAAACCACTTCCTGTGATTCTTTGGTCTATAAAAAGATTAACGGTAACCTCAAAATTGTGTTTGATGAAAACGGCATGCCGATTGTCAAAAAGATTAACGGAAAGAAAGATAGTTGGTTTGACATTAACGGCAAAATCGTGATTAGCCCGGCTTACTATTGCCAAATTGGGTTGTATTTGTACTTGCGCAATATTACCAAAGGAATGTTTGCGATTGCTTTTTTAGAACCACAAGACTATGTGCATCCCGAATGGTTTGAAGCAAAGCAACGCGACATCCGTTTGGTACCAGTACAAATTGATCGCAAGGCCTTTGAAGTTTTAACTAATAAAGCACAGCTGTGGTACAACAGTTTTATTCGTACTGGTAAAAGTCCACAATTAACCTCACAAGACTGGGAGTGA", "aa": "MVKIYEDEFDETLSRAGQVIEPKIRDYVIAKTGFNFHSYDPKEVKWDLFPENPVFGGIPDGEPVDVYGKLAYDTNAPMLEIKTTSCDSLVYKKINGNLKIVFDENGMPIVKKINGKKDSWFDINGKIVISPAYYCQIGLYLYLRNITKGMFAIAFLEPQDYVHPEWFEAKQRDIRLVPVQIDRKAFEVLTNKAQLWYNSFIRTGKSPQLTSQDWE", "aa_hexdigest": "737e749fd61bcb05428dcd1f1dfe05f4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 666123, "stop": 666968, "observations": { "mutation": [ 666149, 666803 ], "stop": [ 666149, 666803 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75227", "query_cov": 1.0, "subject_cov": 0.7651245551601423, "identity": 1.0, "score": 410.0, "evalue": 8.99e-144, "gene_start": 1, "gene_end": 215, "reference_start": 59, "reference_end": 273, "reference_sequence": "MGFTKQYKIDFDIIDNQIVLSNNYFQKHKGSFRKITGTRFGKVIGISEYETSLKTWANMVKIYEDEFDETLSRAGQVIEPKIRDYVIAKTGFNFHSYDPKEVKWDLFPENPVFGGIPDGEPVDVYGKLAYDTNAPMLEIKTTSCDSLVYKKINGNLKIVFDENGMPIVKKINGKKDSWFDSNGKIVISPAYYCQIGLYLYLRNITKGMFAIAFLEPQDYVHPEWFEAKQRDIRLVPVQIDRKAFEVLTNKAQLWYNSFIRTGKSPQLTSQDWEWLRENGIA" }, "paralog": false, "identity": 0.9928825622775801, "score": 534.0, "evalue": 1.11e-194, "description": "Internal stop codon at 666149, 666803. Nonsense mutation around 666149, 666803.", "psc": { "uniref90_id": "UniRef90_P75227", "product": "MPN551 family DNA-binding protein", "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75227", "UniRef:UniRef50_P75227" ] }, "pscc": { "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227" ], "product": "Uncharacterized protein MG373 homolog" } }, "id": "GIHJHMOALN_1175", "locus": "GCA009939745_01174" }, { "type": "cds", "sequence": "contig_1", "start": 666801, "stop": 666968, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTTACCAAACAGTACAAAATCGATTTTGATATTATTGACAACCAAATAGTTTTGTCAAACAACTATTTTCAAAAACATAAAGGTAGTTTTCGCAAGATTACCGGTACCCGATTTGGTAAAGTAATAGGTATTTCTGAGTATGAAACTTCACTTAAAACTTGA", "aa": "MGFTKQYKIDFDIIDNQIVLSNNYFQKHKGSFRKITGTRFGKVIGISEYETSLKT", "aa_hexdigest": "cc6b4530b501482e28ce0c7bb25e98ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6407.267899999999, "isoelectric_point": 9.630819129943848 }, "id": "GIHJHMOALN_1176", "locus": "GCA009939745_01175" }, { "type": "cds", "sequence": "contig_1", "start": 666975, "stop": 667637, "strand": "-", "frame": 2, "gene": null, "product": "MPN552 family protein", "db_xrefs": [ "GO:0005524", "SO:0001217", "UniRef:UniRef50_P75226", "UniRef:UniRef90_P75226" ], "nt": "GTGTATCTGTTAGATTTACTTTCACTCAACAACGTTGATGTAATTGCGGAATCAGGTGCCATTACCTGGTTTTGCAAAACCAATCAGTATGACTATCAAGCCTTTTTAGACCTCGCTAGTATTAATGTCATTATCCACCACGCTGTGATTACCAATTCCGGGATCTTTACCATGGGTAAAAGCCGCGTTGATGATACCGCTAATTTATCAGCTAACTATTTCATTTCACTACAAAAGTACAAGGACTTTAAGTCGCTGTGGTTAACGGACTTTGAACAAACCTTAAAGTACGAAAACTTTTTAAAACAACTCGGTAAATTGGAACTCAGTTCTATCTACGTCTTTAGTCCCCAATACCACATGGACTTAGCTTTTATTGACCAAATTGCTAGTGGTCAACCACGCTTTACCCACAGCAATTTCTATCCCAATAACCTGTTGTTTACAAGTAATAAAGTAACTAAATTTAATGCACTGGAAAAATACACTAAGACCCAAGGCTTAATGCTTAAAGATGTGCACTACATTAACTTAGATGAAACTTTGGTGCAAAACAGTGAGCAGTTAGCTAGTGCTGTTTTCATCAAGAAACAGAACAATGAAGGTTTAACAGTGCAGGACATTCCTGATGTGTTACAAAAGCTTTGTGCACAACTCTTGTAA", "aa": "MYLLDLLSLNNVDVIAESGAITWFCKTNQYDYQAFLDLASINVIIHHAVITNSGIFTMGKSRVDDTANLSANYFISLQKYKDFKSLWLTDFEQTLKYENFLKQLGKLELSSIYVFSPQYHMDLAFIDQIASGQPRFTHSNFYPNNLLFTSNKVTKFNALEKYTKTQGLMLKDVHYINLDETLVQNSEQLASAVFIKKQNNEGLTVQDIPDVLQKLCAQLL", "aa_hexdigest": "ff2b4e6e901bfbcab640c7cd4c669444", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75226", "query_cov": 1.0, "subject_cov": 0.8178438661710037, "identity": 0.995, "score": 438.0, "evalue": 7.68e-155, "valid": true, "product": "MPN552 family protein", "uniref50_id": "UniRef50_P75226", "go_ids": [ "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_P75226", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75226" ], "product": "Uncharacterized protein MG374 homolog" }, "genes": [], "id": "GIHJHMOALN_1177", "locus": "GCA009939745_01176" }, { "type": "cds", "sequence": "contig_1", "start": 667813, "stop": 668073, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGGCACCAGTACAAGTAGTGGTGATTCCGGTTAACATCGATAAACACGAAAAGGCTGCCAAAAAACTGTACCAAAAATTGTTAAAGGAAAACATTAGGGTCAGCCTAGATGAGAGCGAAGATCGTTTGGGCAAAAAAGTTCGCACAGCCATTGTTAACAAGATTCCACTGCAAATTATTGTCGGCGATAAGGAGATGCAGGATCTTACTCGAATAACTTGTCGTGGCTTTAAAGGTGAGAAGGTACAGAAACTGTATTGA", "aa": "MAPVQVVVIPVNIDKHEKAAKKLYQKLLKENIRVSLDESEDRLGKKVRTAIVNKIPLQIIVGDKEMQDLTRITCRGFKGEKVQKLY", "aa_hexdigest": "f0d2dd0b06db86d9cf87c7e0ae22a61e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 80, "aa_cov": 0.9069767441860465, "hmm_cov": 0.9875, "evalue": 1.8356686788419296e-21, "score": 71.61233520507812, "start": 5, "stop": 82 } ], "seq_stats": { "molecular_weight": 9857.613199999996, "isoelectric_point": 9.716755485534666 }, "id": "GIHJHMOALN_1178", "locus": "GCA009939745_01177" }, { "type": "cds", "sequence": "contig_1", "start": 668074, "stop": 668352, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "GTGTTTAAGGAGTTAAATGTGCCCTATAAAATTGATGTGGGTGCAGCTGCTTTTTATGGACCTAAAATTGACTTTCAGTTTAAAACGGCACTCAATAAGATGATTACCATTGCCACTATTCAACTGGATTTTTTACTGCCAGAACGCTTTGAATTGCGTTACTTTGATGAGCAGAGCAATGCTCAAACTCCCGTAATTATTCACGTTGGCATAGTCGGTACGTACGAGCGTTTTATTGCTGCTTTATTGGAAAAAACTCATGGCAATCTACCGTTGTGA", "aa": "MFKELNVPYKIDVGAAAFYGPKIDFQFKTALNKMITIATIQLDFLLPERFELRYFDEQSNAQTPVIIHVGIVGTYERFIAALLEKTHGNLPL", "aa_hexdigest": "fda05000102537b83a3dfef45eb140e3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 72, "aa_cov": 0.7608695652173914, "hmm_cov": 0.9722222222222222, "evalue": 3.769088826751491e-15, "score": 51.888248443603516, "start": 16, "stop": 85 } ], "seq_stats": { "molecular_weight": 10505.126599999989, "isoelectric_point": 6.054114341735839 }, "id": "GIHJHMOALN_1179", "locus": "GCA009939745_01178" }, { "type": "cds", "sequence": "contig_1", "start": 668380, "stop": 668898, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGGAAAAGGGAGCAGTTTTAAAAAAGATTATTGGTGATTTTGTCCACTACCAACAGTTAATGTTTGGTTTTAAAACGGTATCTTCACCAGTATTAGCTAACTTAGAGCTGTACAAGATTAGTGGTCACTATGTGCACTATAGTGAGGACATGTTCCCCTCTGTCAAATTAGAAAACCAAATGATGATGCTCCGGCCAATGACCTGTCCGCACCACTGTTTGATTTTCAAGCACAAACGCCGCTCTTACAAAGAGTTACCACAACGCTTCTGTGAGGATTCCATCTTGCACCGCTTTGAAGCATCGGGTGGGCTTACTGGTTTGGAACGGGTAAGGTGCATGACACTGCTCGACAACCACATCTTTTGTCGTACTGACCAAATTAAAGCTGAGATTAAAAATGCCTTTAAGTTAATCCAAACGGTCAACAACAAGTTTGGTTTTAGCTTTGACCGAATTGACTTGGCCTTACACGATCCTAATAACAAGGCCAAATTCATTGACAACGATCAGTTATGA", "aa": "MEKGAVLKKIIGDFVHYQQLMFGFKTVSSPVLANLELYKISGHYVHYSEDMFPSVKLENQMMMLRPMTCPHHCLIFKHKRRSYKELPQRFCEDSILHRFEASGGLTGLERVRCMTLLDNHIFCRTDQIKAEIKNAFKLIQTVNNKFGFSFDRIDLALHDPNNKAKFIDNDQL", "aa_hexdigest": "2b75d7c9c35898e5159a9b5f73e2e4ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 104, "aa_cov": 0.5930232558139535, "hmm_cov": 0.9807692307692307, "evalue": 2.676937878381744e-20, "score": 68.66114807128906, "start": 58, "stop": 159 } ], "seq_stats": { "molecular_weight": 20113.2449, "isoelectric_point": 8.953191947937015 }, "id": "GIHJHMOALN_1180", "locus": "GCA009939745_01179" }, { "type": "cds", "sequence": "contig_1", "start": 668899, "stop": 669363, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAAGAGCTTTTCCATTAAAGAGTTTGAACAGCTCGAACAGGACTTAAATGCTTATTTTAATCAGTTAAATAATGTTGTTCAGACAAAAATTGATAAGACAAAATCACTCAAATTTTTTCAAGCTGATCCGTTTACAACCACGCTTATTGAAAGTATAGAAAGCAACAGCCTTGTAGTTACCACCGTAAATGAACAAACATTTTGGGTGCATGATTTAGTGTTGCCATTACCCAAACAGGGCTTTGTGAAACTGCTCAATGTTAGTGCCAACTATTTTTTAGGTGATCCCACAAAACCACAACTACAAAGGCTTGTGGGAATTTTTGCAGCCTCTAAAGAACAGCTTAAAAGTCTTATTGCGGCTAATGAACAAAAATACCAAAATGATCACCGTGCTATTGGTAAGCGTTTAGAGATCTTTACCTTTGATCCCTTAGTCGGTGCAGGCTTTCCGATTTGA", "aa": "MEKSFSIKEFEQLEQDLNAYFNQLNNVVQTKIDKTKSLKFFQADPFTTTLIESIESNSLVVTTVNEQTFWVHDLVLPLPKQGFVKLLNVSANYFLGDPTKPQLQRLVGIFAASKEQLKSLIAANEQKYQNDHRAIGKRLEIFTFDPLVGAGFPI", "aa_hexdigest": "5b7ca69845cf2cf926b94364f10abb75", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17524.850199999983, "isoelectric_point": 6.116296195983886 }, "id": "GIHJHMOALN_1181", "locus": "GCA009939745_01180" }, { "type": "cds", "sequence": "contig_1", "start": 669543, "stop": 669698, "strand": "-", "frame": 2, "gene": null, "product": "putative MG376-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224", "UniRef:UniRef90_P75224" ], "nt": "ATGCGCTTTTTTGGCGAGCGTTTATTTACTGACTATTACGTCATTTATGCCAATGGTCAACTGGCTTACGAATTGGAGAAACACACCAAGAAGTACAAGACCATTAGTATTGAAGGCATTTTGCGCACTTATTTAGAGCGTAAGAGTGAAATTTGA", "aa": "MRFFGERLFTDYYVIYANGQLAYELEKHTKKYKTISIEGILRTYLERKSEI", "aa_hexdigest": "46c870f8326c63aabc3fc298666ec418", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 669471, "stop": 669785, "observations": { "mutation": [ 669545, 669740 ], "stop": [ 669545, 669740 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75224", "query_cov": 1.0, "subject_cov": 0.4636363636363636, "identity": 1.0, "score": 102.0, "evalue": 5.16e-27, "gene_start": 1, "gene_end": 51, "reference_start": 36, "reference_end": 86, "reference_sequence": "GGGGGGMLNRVFLEGEIESSCWSVKKTGFLVTIKQMRFFGERLFTDYYVIYANGQLAYELEKHTKKYKTISIEGILRTYLERKSEIWKTTIEIVKIFNPKNEIVIDYKEI" }, "paralog": false, "identity": 0.9807692307692307, "score": 200.0, "evalue": 4.32e-68, "description": "Internal stop codon at 669545, 669740. Nonsense mutation around 669545, 669740.", "psc": { "uniref90_id": "UniRef90_P75224", "product": "Uncharacterized protein MG376 homolog", "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75224", "UniRef:UniRef50_P75224" ] }, "pscc": { "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224" ], "product": "Uncharacterized protein MG376 homolog" } }, "id": "GIHJHMOALN_1182", "locus": "GCA009939745_01181" }, { "type": "cds", "sequence": "contig_1", "start": 669787, "stop": 670368, "strand": "-", "frame": 1, "gene": null, "product": "MPN555 family protein chaperone", "db_xrefs": [ "GO:0006457", "GO:0015031", "RefSeq:WP_014325598.1", "SO:0001217", "UniParc:UPI0001DD642D", "UniRef:UniRef100_A0AAX0SSF1", "UniRef:UniRef50_P75223", "UniRef:UniRef90_P75223" ], "nt": "ATGGCTACAAATCTTAAATCAACCGCTAAACTAGTTAAACCAATTCAGTACGATAAGGTAATTGAAGTAGAACGCATCTTTGCTGACCCTGCTTTTATTGAACAACACCGCCAACGGATCTTAGCTTCCTTTAAGGACGCTAAGGAAAGTGCGCTTTATCATGAACTAACGCACATTGTCATTAAGGACAACCTCTTTTCCTGTGCCATGAATGCTATTGTGGGTTACTTTGAATTCAACATCGATGAAGCTGAATTGAAGAATGTCATGGAAGGTCTAAAGCGTGATGTGATTCAGGGCGCTGAGGACAACACCGTGCAAGCAATTGCTGAAAAAATTATTAAGAAGGCTTTAGTGTTTAACCACCTCCAAAAAGAATGGAAAGTGGAAATCACTGATGAAGTGGTGAAGAATGTAATTAGTCTTTATTACGAAAAAACCAATCAAAGTGTGCGCGAATACTTGGATGATAAACAAAAGTTTGAGGGTGTGCGTACAGCCTTGCTAGAAGAGCGCATGGTGTTAGAAACCATCAACCACTTTAAATTTCACTTTAACTTAACTGGACAATTACCAAACTAA", "aa": "MATNLKSTAKLVKPIQYDKVIEVERIFADPAFIEQHRQRILASFKDAKESALYHELTHIVIKDNLFSCAMNAIVGYFEFNIDEAELKNVMEGLKRDVIQGAEDNTVQAIAEKIIKKALVFNHLQKEWKVEITDEVVKNVISLYYEKTNQSVREYLDDKQKFEGVRTALLEERMVLETINHFKFHFNLTGQLPN", "aa_hexdigest": "21a9954fee74d8d30aa974a9214b512b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD642D", "ncbi_nrp_id": "WP_014325598.1", "uniref100_id": "UniRef100_A0AAX0SSF1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD642D", "RefSeq:WP_014325598.1", "UniRef:UniRef100_A0AAX0SSF1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SSF1", "uniref90_id": "UniRef90_P75223" }, "psc": { "uniref90_id": "UniRef90_P75223", "product": "MPN555 family protein chaperone", "uniref50_id": "UniRef50_P75223", "go_ids": [ "GO:0006457", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75223" ], "product": "Uncharacterized protein MG377 homolog" }, "genes": [], "id": "GIHJHMOALN_1183", "locus": "GCA009939745_01182" }, { "type": "cds", "sequence": "contig_1", "start": 670411, "stop": 671199, "strand": "-", "frame": 1, "gene": "argS", "product": "arginine--tRNA ligase", "db_xrefs": [ "COG:COG0018", "COG:J", "EC:6.1.1.19", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420", "KEGG:K01887", "SO:0001217", "UniRef:UniRef50_P47618", "UniRef:UniRef90_P75222" ], "nt": "TTGAAGACTACGCTCTATGGTGATGATAAGGACCGGGTGTTAGTGCGTCAAGACAATCGGCCTTCTTACTTTGGTACCGATGTCGCTTACCACTTAGACAAGGCTGCGCGGGGCTTTGACCTCTTATACGACATTTGGGGCAGTGATCATGAAGGGCACATTAAACGGATGCACTGTGTTTATGAGGGACTAAAGATTCACCAAAAATGCCAGCTCAAAATTACTGCCTTACAGTTGGTAATGCTGTACAAGAATAAGGAAATAGTCAGATTGTCCAAACGTGCTGGCAACGTTATTACCATTAAGCAAATGTTGCAAATGCTCAGTGAGGATGCGGCGCGTTGGTTTATGTTGTCGCAAACCAACAACTCGATTATCAAGATTGATTTGGACACTGCTAACCTGCAAAACTCTTCCAATCCGGTTTATTACGTGCAATATGCGTATGCCAGAATGTGCAGTGTATTAAAGGTAGTGGATCAAGCACCACTAGCTCAAGTAAATGACTGCAGCTTATTAACGCATGAAAAGGAAATTGCTTTACTAGACCAGTTAGTGTACTTTAAGAGCTTACTGGAAAAAGTTCAGGTTAGCCATGAACTGCACCTCTTAACTAATTACTTATACGAGACAGCTACCTTGTTTCATTCTTGGTATAAGGCTTGCAAAATTAACGATCCAGCGCAGTACAATTTAACCCAGCAACGCTTACTTTTACTGCAATCATTGCACCATGTTTTTGGTCAGCTATTGCAAATCTTAAACATTACTGCACCACAACAAATGTAA", "aa": "MKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAPLAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM", "aa_hexdigest": "e9e16ec669ea9b851d64bc7e0005568b", "start_type": "TTG", "rbs_motif": null, "genes": [ "argS" ], "truncated": "3-prime", "pseudogene": { "start": 670411, "stop": 671499, "observations": { "mutation": [ 671202 ], "stop": [ 671202 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75222", "query_cov": 1.0, "subject_cov": 0.48789571694599626, "identity": 0.992, "score": 518.0, "evalue": 1.62e-181, "gene_start": 1, "gene_end": 262, "reference_start": 276, "reference_end": 537, "reference_sequence": "MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDKLSFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAIWLKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAALAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM" }, "paralog": false, "identity": 0.988950276243094, "score": 721.0, "evalue": 1.3e-263, "description": "Internal stop codon at 671202. Nonsense mutation around 671202.", "psc": { "uniref90_id": "UniRef90_P75222", "gene": "argS", "product": "arginine--tRNA ligase", "ec_ids": [ "6.1.1.19" ], "uniref50_id": "UniRef50_P47618", "cog_id": "COG0018", "cog_category": "J", "kegg_orthology_id": "K01887", "go_ids": [ "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75222", "EC:6.1.1.19", "UniRef:UniRef50_P47618", "COG:COG0018", "COG:J", "KEGG:K01887", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ] }, "pscc": { "uniref50_id": "UniRef50_P47618", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47618" ], "product": "Arginine--tRNA ligase" } }, "id": "GIHJHMOALN_1184", "locus": "GCA009939745_01183" }, { "type": "cds", "sequence": "contig_1", "start": 671200, "stop": 672024, "strand": "-", "frame": 1, "gene": "argS", "product": "arginine--tRNA ligase", "db_xrefs": [ "COG:COG0018", "COG:J", "EC:6.1.1.19", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420", "KEGG:K01887", "SO:0001217", "UniRef:UniRef50_P47618", "UniRef:UniRef90_P75222" ], "nt": "ATGCTTTTCATTAACACAGATCTTCAAGAATGTCTTAACGCTTTGAATCTAGAATTTGATGAGCACAAAGAGTTAGTCAAACTTGTTAAAAACAACAGCTTTAGTGGTTTTGCTTCCACCGTTGTGTTCCACTTAAAGGGTGTAAATCAAAAGGAAACCGCCCAACAGATTGCTGCTTGGTTGTTAAAACACAAAAAGGCGCATTACCGCCGTGTGTTTGTCGCTAATAACAACTTCATTAACTTTGAAATTAGCCCTCAAAAATACCTCGATTTTTTAAAAACCAAACCTACTTTTGCCCCTAAACCAACTAAGGTTCTAATTGAATGGGTGTCGGCTAACCCCACGGGTGAGTTGCACTTAGGTCATGTCCGCAATGCCTTCTTTGGTCATGTACTCAATAACCTGATGGTTTTTTTAGGCTTTCAAACAGTTCGGGAATACTGGGTCAATGACTATGGTCAACAAGCACGGGTGTTTGGTTTTAGTGTGTACCAAGCACTCCACTTACAACAAAACATTAAGGTCACTCCTCACCCTGATGGTTATGAGGGGGAGCTAGTGGACAGTATTGCCAAAACTATTACCGGTATTCCTTTAGATAACATCAGCTTTGAAGAGTTTTTACAACAACCCTTTCTGGACCAGTTACTAGCTGACTGTACTGCCAAAGTCTTGGAAGTAATTAAGCAAGACTTAGCTACAATCCACATTCACTTTGACAGCTGGAAGTTTGAAAGTCAAGTGGTTAAGGAAACAGATTACAAAAAACTGTTAACCCAGTTTAAGGACGAAGCGCACTATGAAAAGGATGGTGCCATTTGA", "aa": "MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDNISFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAI", "aa_hexdigest": "d647fd5d485d3172661a27ac2facc7e1", "start_type": "ATG", "rbs_motif": null, "genes": [ "argS" ], "truncated": "5-prime", "pseudogene": { "start": 670897, "stop": 672024, "observations": { "mutation": [ 671202 ], "stop": [ 671202 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75222", "query_cov": 1.0, "subject_cov": 0.5102420856610801, "identity": 0.993, "score": 552.0, "evalue": 7.94e-195, "gene_start": 1, "gene_end": 274, "reference_start": 1, "reference_end": 274, "reference_sequence": "MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDKLSFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAIWLKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAALAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM" }, "paralog": false, "identity": 0.992, "score": 760.0, "evalue": 6.33e-279, "description": "Internal stop codon at 671202. Nonsense mutation around 671202.", "psc": { "uniref90_id": "UniRef90_P75222", "gene": "argS", "product": "arginine--tRNA ligase", "ec_ids": [ "6.1.1.19" ], "uniref50_id": "UniRef50_P47618", "cog_id": "COG0018", "cog_category": "J", "kegg_orthology_id": "K01887", "go_ids": [ "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75222", "EC:6.1.1.19", "UniRef:UniRef50_P47618", "COG:COG0018", "COG:J", "KEGG:K01887", "GO:0004814", "GO:0005524", "GO:0005737", "GO:0006420" ] }, "pscc": { "uniref50_id": "UniRef50_P47618", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47618" ], "product": "Arginine--tRNA ligase" } }, "id": "GIHJHMOALN_1185", "locus": "GCA009939745_01184" }, { "type": "cds", "sequence": "contig_1", "start": 672052, "stop": 673890, "strand": "+", "frame": 1, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "COG:COG0445", "COG:J", "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660", "KEGG:K03495", "RefSeq:WP_010874914.1", "SO:0001217", "UniParc:UPI000012B445", "UniRef:UniRef100_P75221", "UniRef:UniRef50_P75221", "UniRef:UniRef90_P75221" ], "nt": "ATGTCTTTTACACTAACAGTTATTGGTGGCGGTCATGCCGGATTAGAGGCCGCATTTATTGCTAGTAAACTAGGGCTAAAAGTTAACCTCTTAGTACTAGATCCCAACCATGTGGGGTCGTGTCCCTGTAACCCGGCCATAGGAGGTCCTGCTAAAGGTATAGTAACCCGGGAAATCGATGTTTTAGGAGGGATGCAGGGTAAGGCCGCTGATGCCACTGCTTTACAATACAAGCTGCTCAACAGCTCGAAAGGTCCAGCAGTGCAAGCAATCCGCGCTCAAATAGACAAAATTGCTTATCAGAAGTGGTTTCGCCAGCAAATTGACCAAACGCCTAACATTGAGTTAATTGCTGGGGAAGCCGTTGATATCCTTGAAAGTAACGGTAAAGTTAAAGGTGTCGTGTTAGCTGATGGTAGTGAACTGGCATCAGATGCTGTTATTGTGACTACGGGAACTTATCTAAAGGCCAAAACTTACTGTGGTAGCTTAAGCAAAGAAGAAGGTCCTGACCGCGCTAAGCGCAGTGAATACCTTTCCACTAACTTAATTAAGCGGGGTTTTAAAACGTTACGACTAAAAACTGGGACACCACCACGGATCCTACGCGAATCACTCGACTTTAGCCAAATGGCTGTGGAAGCTAACACTACCCCTCATTTAGCCTTTAGCTTTACTACTAAGAATTATTTACCACTAGAACAGCAAGTGATCTGTCACTTAATCCATACCAATCCCCAGATCCATCAGTTAATTCTGGCCAACTTAAAACAGTCCGCTGTCTTTAACGGTAGTATTAAAGCAAACGGACCACTTTACTGTCCGAGTATTGAAGACAAGGTATTTCGCTTTCAAGACAAAGAACGGCACCAAATCTTTGTCGAACCTGAATCACTTAGTTTAGAAACGGTTTACCTAGCAGGGTTTTCCACTTCCTTTCCCCCAGAGGTGCAAGAGCACATTGTCCGTTTACTCCCTGGGTTTAAAAATGCCCGGTTTCAAAAGTATGGCTATGCCATTGAATATGATGCCTTTAGTTCGATACAACTCAAATCAACACTAGAAACTAAGTTAATTCAAAATCTGTATTTTGCCGGACAGATTAATGGTACCAGTGGGTATGAGGAAGCAGCTGGCCAGGGCTTAATCGCTGGGATTAACGCCGCTTTAAAGCTACAGCGTAAACCCGAATTTGTGTTACAGCGCAATGAGGCATACCTTGGTGTCATGATTAACGACCTGGTTACTAAGGAAATTAGTGATCCCTACCGCTTGTTAACTTCGCGCGCTGAACACCGTTTGTGGTTGCGCAATGATAACTTACAAGAACGTCTAATTGAAAAGAGCCGAGCCTTAGGTTTAGTGGAAGCAGATGTGTATGCCAATTACTTGGAACAACAACAAAAGAAAAAACAATTAATTGATTATTTACAAACCACTACCGTTGGGCAAATTGCTGCCTTGAAATTAAACTTTAAAAACACGGCCCAAACGCTGTTTGACTTTACCAAGCGTGCTGAAATTAAGCTGGTTGATTTAGTACAACTACTCCCCAAGCGTTTTGACTTGGATGTACAAAGTTTAAACCAGATTGACATTGACATTAAGTACGCTGGTTACATTAAGAAGAGTGAAAAGTACTTCAAGAGTTTAAACAACCTCAGTAGTGTCAAAATTCCCTTAAAGTTAAATTACCACAAAGTACCTAACTTAGCGAGTGAAGCAATTGTTAAACTCTCCAAAATTAGACCAACTGATCTCAGTGTAGCTAGTCAAGTAGCGGGCATTAACTTTAACGATATTTTAGCGATTAAACATTTTTTAGATAACCATGAATAA", "aa": "MSFTLTVIGGGHAGLEAAFIASKLGLKVNLLVLDPNHVGSCPCNPAIGGPAKGIVTREIDVLGGMQGKAADATALQYKLLNSSKGPAVQAIRAQIDKIAYQKWFRQQIDQTPNIELIAGEAVDILESNGKVKGVVLADGSELASDAVIVTTGTYLKAKTYCGSLSKEEGPDRAKRSEYLSTNLIKRGFKTLRLKTGTPPRILRESLDFSQMAVEANTTPHLAFSFTTKNYLPLEQQVICHLIHTNPQIHQLILANLKQSAVFNGSIKANGPLYCPSIEDKVFRFQDKERHQIFVEPESLSLETVYLAGFSTSFPPEVQEHIVRLLPGFKNARFQKYGYAIEYDAFSSIQLKSTLETKLIQNLYFAGQINGTSGYEEAAGQGLIAGINAALKLQRKPEFVLQRNEAYLGVMINDLVTKEISDPYRLLTSRAEHRLWLRNDNLQERLIEKSRALGLVEADVYANYLEQQQKKKQLIDYLQTTTVGQIAALKLNFKNTAQTLFDFTKRAEIKLVDLVQLLPKRFDLDVQSLNQIDIDIKYAGYIKKSEKYFKSLNNLSSVKIPLKLNYHKVPNLASEAIVKLSKIRPTDLSVASQVAGINFNDILAIKHFLDNHE", "aa_hexdigest": "60eda10628024d38f59690539339f17b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B445", "ncbi_nrp_id": "WP_010874914.1", "uniref100_id": "UniRef100_P75221", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B445", "RefSeq:WP_010874914.1", "UniRef:UniRef100_P75221" ] }, "ips": { "uniref100_id": "UniRef100_P75221", "uniref90_id": "UniRef90_P75221" }, "psc": { "uniref90_id": "UniRef90_P75221", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_P75221", "cog_id": "COG0445", "cog_category": "J", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0005829", "GO:0008033", "GO:0030488", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75221", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75221" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" }, "genes": [ "mnmG" ], "id": "GIHJHMOALN_1186", "locus": "GCA009939745_01185" }, { "type": "cds", "sequence": "contig_1", "start": 673883, "stop": 674458, "strand": "+", "frame": 2, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "COG:COG0357", "COG:J", "EC:2.1.1.-", "EC:2.1.1.170", "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043", "KEGG:K03501", "RefSeq:WP_010874915.1", "SO:0001217", "UniParc:UPI000012B477", "UniRef:UniRef100_P75220", "UniRef:UniRef50_P75220", "UniRef:UniRef90_P75220" ], "nt": "ATGAATAACAGTAAATTGAAACAGTATGTTCAACTAGTACAAACAGCCAACCAAAACTTTAACCTCACCGGTCTTAAAACTGAAGGCGAAATTTACGAACACTTAGTCCAAGAAATTATCGAGCTCTTTAATGAGTATGACAGTTACTTTGACCATAAAAAGGTTGCAGATTTGGGTAGTGGAAATGGTTGTCCTGGTGTCATCTTAAAGCTGTTGTTTCCCCAAATCAAAACTTTGGATTTAATTGATAGCAAACACAAAAAGGTTAACTTTTTAAAAGAGGTGATTCAAACACTGGAACTAAACAATACCCAAGCACTTTGTGCCAGAATTGAAAACCACACTGAACAATACGACACTTTATGCTCCCGCGGACTTGGCAGCATTATTGAGGTTAATGCCTTTGCTTTAAAACTGTTAAAGCCCAACGGCATTATCTTCCACATTAAACAAAGCTTAGATCAATACCTCGAGTTTGAGGATTCGGAGCAAAAAGACCAGTTTAAACCACTGTTCTTTAAGTTTTTCCATGGCAAGCGCCAGCAAATCCTGATTGCCATGAAGAAAAATGTTTAA", "aa": "MNNSKLKQYVQLVQTANQNFNLTGLKTEGEIYEHLVQEIIELFNEYDSYFDHKKVADLGSGNGCPGVILKLLFPQIKTLDLIDSKHKKVNFLKEVIQTLELNNTQALCARIENHTEQYDTLCSRGLGSIIEVNAFALKLLKPNGIIFHIKQSLDQYLEFEDSEQKDQFKPLFFKFFHGKRQQILIAMKKNV", "aa_hexdigest": "be8301c57ed36447e490b8d2609dca06", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B477", "ncbi_nrp_id": "WP_010874915.1", "uniref100_id": "UniRef100_P75220", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B477", "RefSeq:WP_010874915.1", "UniRef:UniRef100_P75220" ] }, "ips": { "uniref100_id": "UniRef100_P75220", "uniref90_id": "UniRef90_P75220" }, "psc": { "uniref90_id": "UniRef90_P75220", "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.-", "2.1.1.170" ], "uniref50_id": "UniRef50_P75220", "cog_id": "COG0357", "cog_category": "J", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043" ] }, "pscc": { "uniref50_id": "UniRef50_P75220", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75220" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "GIHJHMOALN_1187", "locus": "GCA009939745_01186" }, { "type": "cds", "sequence": "contig_1", "start": 674508, "stop": 674660, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTGCCAAACAGTTTTATGTAACTAGTAGTGCTGGTGCAATTCTGTTTGACCTTAAAAAGAGTCAGGGCTTAGACCAACTGCAAGCAATTGAAAAAACAAGGAAGGTTATTATTACAGAACAGTTTGCCAACCAAACTGGTAAATGA", "aa": "MEFAKQFYVTSSAGAILFDLKKSQGLDQLQAIEKTRKVIITEQFANQTGK", "aa_hexdigest": "71181d2970941635dc4be202fd1bff47", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5622.407499999999, "isoelectric_point": 9.22402362823486 }, "id": "GIHJHMOALN_1188", "locus": "GCA009939745_01187" }, { "type": "cds", "sequence": "contig_1", "start": 674664, "stop": 674885, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGACAAGGAAAACACTAAACGGATTAACAGCTTAGCGCAAGAGCACATTACTCCCTTAATAAAACGGATTTTACGCTTAGCGGACTTTAAAAATGTCTTGATTAATGTGCAGCACCACAAGAAGCTGCAAAAATGTCTGTTATGGGAAATTAACGGTTTAATCTGCTTAGTTGAAAGTTTACAGTTCATGGAAAATCCCACTGCAATCATGGAATGA", "aa": "MFDKENTKRINSLAQEHITPLIKRILRLADFKNVLINVQHHKKLQKCLLWEINGLICLVESLQFMENPTAIME", "aa_hexdigest": "907136105473540eec5b93e8c71ce90e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8614.161799999998, "isoelectric_point": 8.712273597717285 }, "id": "GIHJHMOALN_1189", "locus": "GCA009939745_01188" }, { "type": "tRNA", "sequence": "contig_1", "start": 675120, "stop": 675195, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 74.8, "nt": "GCGTCGGTAGCTCAGCGGATAGAGTACACGCCTTCTAAGTGTGTTGTCGTGGGTTCGAGTCCCACCCGATGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 675160, 675162 ], "id": "GIHJHMOALN_17", "locus": "GCA009939745_01189" }, { "type": "cds", "sequence": "contig_1", "start": 675197, "stop": 676513, "strand": "-", "frame": 3, "gene": "arcA", "product": "Arginine deiminase-like protein", "db_xrefs": [ "COG:COG2235", "COG:E", "GO:0016990", "GO:0019546", "RefSeq:WP_010874917.1", "SO:0001217", "UniParc:UPI0000125DB1", "UniRef:UniRef100_P75218", "UniRef:UniRef50_P75218", "UniRef:UniRef90_P75218" ], "nt": "ATGTCAAAAAAACAGCTTGTCAAAACCGATGGGCATAACCAATTAGATCAACCAAACACTAAAGCACTACAGTTAAAAAAGAAGCAGTTTAACAGTGGTGTACGCGTTACTAGTGAAATTAGTTTTTTAAGGGAAGTAATTGCCCATCATCCCGGTATCGAAACCGAACGAGTGATTGATAACCAGACCTTTGGCAGTGCAATGTACTTAGAACGCGCTCAAAAGGAACACCAGTTATTTATTAAGATTTTGCGCCAACATGGTACAAAGGTGCACTATTTACAAGATTTACTGTTAGAAGCTTTAAGTGCGGCTGACCCTAATGTGCGCCAGGACTTCATTAAGAACTTTTTATTGGAATCAGGCATTAAAAGTGTGAGTACCTTCGAAGCTTGCTTAAACTTCTTTCGCAGTTTAGATTCGCTTGTAGATGTGATTAAGGTCATGTTTGGTGGCATTAAGGTATCAGATGTACCACCGATTACCCCGCAACGGTTTGCCGACATTCATGTAAGTAATTCACCGTTTTTAATTAAACCGCTATCGTTTTCCCTTTATCCACATAAGTTCTTCAATACCCTAGGAACGGGTGTCGCTTTATTTGTCACTAATGATTCTGAGTTAAAGCGCCATTCGTTAGTGTATGAATACATCATGCGCTTTCACCCCCGTTTTGATGGAGTTAAGCTTTATACTAACCGTGACTTTAAGAACTGTTTGATTAACAGTAGTGACATTATTCAAATTAGCAATGAAATCTTGCTAATTGGTATTAGTCATGACACCGATGTGTTGGGAATTGAAAGCTTGGCACGTAATTTATTAAGCGATCACACTAATCCAATTAAGCAGATTATTGCAATTAATATCCACAAGTTTGGTGCTAAGACCAATCTCAATAAACTTATAGCGATGGTGGATGTCGACAAGTTCATTATTGCGCGCAAAGTGTTACAAGCTACCGAAATCTTTGAACTCACAGCCACCGCCCAGAGGGATGTGGATGGCTTAGCACAAATTAAATTCAAACCGCTAAAGTTTAATTTTGGTGAGATAATTGAAGCGATTATTGATAAACAACCCCGCTTTGTTATTATTGGTGGTGGTGATGAAGTGGCTGAGCGTAAAGAACTCTTGGACTGTGGTATGGGCGTATTAAACTTAAGCCCTGGAGAAATTGTGGTCTTTGATCGCAACCACTACACCAATAATCTTCTCAATGAACTGGGATTAATCATCCATAAGATCCCCGCTTCCGAACTTTCACGGGGTCCTAGTGGCCCCTTGGAAATGGTTTGCTCTCTCTGGAGAGAATAA", "aa": "MSKKQLVKTDGHNQLDQPNTKALQLKKKQFNSGVRVTSEISFLREVIAHHPGIETERVIDNQTFGSAMYLERAQKEHQLFIKILRQHGTKVHYLQDLLLEALSAADPNVRQDFIKNFLLESGIKSVSTFEACLNFFRSLDSLVDVIKVMFGGIKVSDVPPITPQRFADIHVSNSPFLIKPLSFSLYPHKFFNTLGTGVALFVTNDSELKRHSLVYEYIMRFHPRFDGVKLYTNRDFKNCLINSSDIIQISNEILLIGISHDTDVLGIESLARNLLSDHTNPIKQIIAINIHKFGAKTNLNKLIAMVDVDKFIIARKVLQATEIFELTATAQRDVDGLAQIKFKPLKFNFGEIIEAIIDKQPRFVIIGGGDEVAERKELLDCGMGVLNLSPGEIVVFDRNHYTNNLLNELGLIIHKIPASELSRGPSGPLEMVCSLWRE", "aa_hexdigest": "779244268347b9d84ffb03fd1fa41faa", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0000125DB1", "ncbi_nrp_id": "WP_010874917.1", "uniref100_id": "UniRef100_P75218", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125DB1", "RefSeq:WP_010874917.1", "UniRef:UniRef100_P75218" ] }, "ips": { "uniref100_id": "UniRef100_P75218", "uniref90_id": "UniRef90_P75218" }, "psc": { "uniref90_id": "UniRef90_P75218", "gene": "ArcA", "product": "Arginine deiminase-like protein", "uniref50_id": "UniRef50_P75218", "cog_id": "COG2235", "cog_category": "E", "go_ids": [ "GO:0016990", "GO:0019546" ] }, "pscc": { "uniref50_id": "UniRef50_P75218", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75218" ], "product": "Arginine deiminase-like protein" }, "genes": [ "arcA" ], "id": "GIHJHMOALN_1190", "locus": "GCA009939745_01190" }, { "type": "cds", "sequence": "contig_1", "start": 676567, "stop": 676773, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00485.24" ], "nt": "ATGGATAGTAAAAAAGGAATTTTAGTGGCAATTGGTGGCGGGAGTTGTTCTGGTAAAACTACGATCGCTGACATGATTTACCAACTTTTGCGTAAAAAGCTTAAAGTTGCTATTCTGCCCCAGGACAACTATTACAAACCATACAAGAACAAATCAATGGCGCAACGCAAAGCGATTAACTTCGATCACCCCGATGCCTTTGATTGA", "aa": "MDSKKGILVAIGGGSCSGKTTIADMIYQLLRKKLKVAILPQDNYYKPYKNKSMAQRKAINFDHPDAFD", "aa_hexdigest": "e1ab7c080a2409d72ea9958a36166eff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00485.24", "name": "PRK", "length": 69, "aa_cov": 0.8823529411764706, "hmm_cov": 1.0, "evalue": 6.783704920322642e-07, "score": 24.76234245300293, "start": 9, "stop": 68 } ], "seq_stats": { "molecular_weight": 7606.822200000001, "isoelectric_point": 9.629658699035645 }, "id": "GIHJHMOALN_1191", "locus": "GCA009939745_01191" }, { "type": "cds", "sequence": "contig_1", "start": 676807, "stop": 677052, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00485.24" ], "nt": "ATGGGTAAAACAGTGGCTGTGCCAATGTATGACTATGTTAATTACACCCGTAAAAAGGAAACCATAGAAATAGGTCCTTTAAATGTAGTCATCTTGGAAGGTTTAATGCCGTGGTTTGATGAACGGATTGCTAAATTGTGTAAGCTCAAGATCTTTGTGGAAGCGACTGGAGAGGAACGCTTAATTCGCCGGATTGAACGGGACTGGGAACGCGGTCGTGATGTCGCTTCTATCATTAAACAATGA", "aa": "MGKTVAVPMYDYVNYTRKKETIEIGPLNVVILEGLMPWFDERIAKLCKLKIFVEATGEERLIRRIERDWERGRDVASIIKQ", "aa_hexdigest": "77dc1989918fd2cfa2d3ab0aab7eea23", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00485.24", "name": "PRK", "length": 81, "aa_cov": 0.9876543209876543, "hmm_cov": 1.0, "evalue": 2.7934872115005956e-18, "score": 61.91554260253906, "start": 2, "stop": 81 } ], "seq_stats": { "molecular_weight": 9540.122499999992, "isoelectric_point": 8.816132164001466 }, "id": "GIHJHMOALN_1192", "locus": "GCA009939745_01192" }, { "type": "cds", "sequence": "contig_1", "start": 677071, "stop": 677208, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGAAATCTTTGTCGAAAAGATGAAACAGAAGGCAGATTTAATTATTCCCTGGAGTGAAAGACACGAAGTGAGTACTAATGTGCTCGACTTTGCAATTGAACACCTCTTTCGCAAACACGTGGATCCCAACTAG", "aa": "MYEIFVEKMKQKADLIIPWSERHEVSTNVLDFAIEHLFRKHVDPN", "aa_hexdigest": "6d882a79fb617e52dbfdf00aca6704cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5455.228799999999, "isoelectric_point": 5.80777416229248 }, "id": "GIHJHMOALN_1193", "locus": "GCA009939745_01193" }, { "type": "cds", "sequence": "contig_1", "start": 677205, "stop": 677870, "strand": "-", "frame": 2, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "COG:COG0171", "COG:H", "EC:6.3.1.5", "GO:0003952", "GO:0004359", "GO:0005524", "GO:0005737", "GO:0008795", "GO:0009435", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47623", "UniRef:UniRef90_P75216" ], "nt": "GTGGTTGTTGGATTGTCAGGTGGAGTTGATTCGGCAGTTGTAGCCGCCATGGCTAAAAAACTGTTTCCGCAAAACCACTTAACCTTGGTAATGCACATTAATAACTCCGAGTTAGATCACAAGGCCACAACTGCCTTAGTGGAACAATTACAACTCAACAATAAACAGGTGGATTTAGAACCACCTTACCGCGCTATGTTGCAAGCATTGACCATTGATCCCCAAAAGGAGTTAATGGTAGCCGGCAACCTCAAAGCACGTTTACGAATGGCTTGTCTATACACCCATGCCCAAAAGCACAACTACTTAGTTTTGGGTACTGGCAACTTCATTGAGTACAGCTTGGGCTACTTTACTAAATGGGGTGATGGGGCGTGTGATGTGGCACCCTTAGCGTTCTTGTTAAAGAGCGATGTTTATGCTTTATCCCAGCACTTCAATGTTCCTGAATTGGTCATTGAACGAGCCCCTACTGCTAGTCTGTTTGCCGGGCAGACTGACGAAGCGGAAATGGGTTTGACGTATAAGGAATTGGACCAGTACTTTCAGGGCCACTTACAGTTAAGTGCCACGAAACAACAACGGGTTGACCACTTGCGCCAAAGCTCCCAGCACAAGCGTAGTTTACCTAAAACCTTTAAACCACTTTATTCCTTCCAAATCTAG", "aa": "MVVGLSGGVDSAVVAAMAKKLFPQNHLTLVMHINNSELDHKATTALVEQLQLNNKQVDLEPPYRAMLQALTIDPQKELMVAGNLKARLRMACLYTHAQKHNYLVLGTGNFIEYSLGYFTKWGDGACDVAPLAFLLKSDVYALSQHFNVPELVIERAPTASLFAGQTDEAEMGLTYKELDQYFQGHLQLSATKQQRVDHLRQSSQHKRSLPKTFKPLYSFQI", "aa_hexdigest": "c08c3536d2d321b9d2b7bff552278c12", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75216", "query_cov": 1.0, "subject_cov": 0.8911290322580645, "identity": 0.991, "score": 431.0, "evalue": 2.08e-152, "valid": true, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "ec_ids": [ "6.3.1.5" ], "uniref50_id": "UniRef50_P47623", "cog_id": "COG0171", "cog_category": "H", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0004359", "GO:0046872", "GO:0003952", "GO:0008795", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_P47623", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47623" ], "product": "NH(3)-dependent NAD(+) synthetase" }, "genes": [ "nadE" ], "id": "GIHJHMOALN_1194", "locus": "GCA009939745_01194" }, { "type": "cds", "sequence": "contig_1", "start": 677944, "stop": 679110, "strand": "-", "frame": 1, "gene": "obgE", "product": "GTPase ObgE", "db_xrefs": [ "COG:COG0536", "COG:DL", "EC:3.6.5.-", "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_P75215", "UniRef:UniRef90_P75215" ], "nt": "GTGTTGCAAGCCGACCACAACTGCGATTCCCTCTTTTTTTTAAAGAATAAAAAACACCTGTTTGCTGAAAGTGGCGGCAATGGCAAACCCGATTTAGCCCATGGTAAAAACGGGGAAGATTTGGTTATTAAGGTACCAGTGGGAACTACGGTGCGTGACCTTGATACTAACCAGATCTTAATGGACTTTGTTCATGACCAACAGAGCTTTATTCTCTGTTACGGTGGCAAGGGCGGTAAGGGCAACGCAGCTTTTAAAAGTCCAATTATGCGCGCTCCCAATCTGTATGAAAACGGCGACAAGGGTCAAAGTTTGCATGTGAGCTTGGAAATAAAGTATTTAGCTAATGTGGGGATTGTGGGTTTTCCCAACACCGGTAAGTCCACTTTAATTTCCAAACTGTCCAACGCTAAACCCAAGATTGCTAACTACCGTTTTACAACCTTAGTGCCCGTATTGGGGGTAGTTAAACACAACGACCAAAGTTTGGTGTTTGCTGATATTCCCGGGTTAATTGAAAACGCCAGTGAGGGTAGTGGTCTGGGTCACTATTTCCTGCGCCACATTGAGCGTTGTGAAATCCTAATTCACTTAATTTCACTCGATCCCGTGGATCATGACGATCCTTGTCAAGCGTATGAACAAATTATGCGTGAACTGTCGAAGTACTCCCAGCTGTTAGTTAAAAAGAAGATGCTGGTCGTGGCCAATAAAACAGACGTTGACCTTGATGGTACCCGTTTTCAAAAGTTAGCACAATACCTCGAAAATAAAGGAATTCCCTTGTTCAAAATTAGTGCACTCAAACAAGAACTGGGTGATCTAGTGGCCCAAGTGTTTGCGCTCCACCAAAAAACGCTAGCGCAATTCGGTGCCAATAAGTTTCATTTACCCATGGAAATGGAGAAGCACTATGTCTTTGAACAGGCCAGTGAAACCGACCATGATCCACTCAACATTGAACGTGATGCCTTGGGGCGGTGGCATGTTGAATGCAAGCGTTTACATTATTGGTTTGACAAGATTCCCCAAACTACACTTGACAACATTCGCCGTTTAGGTAATAAGATTAAAGAAGTGGGCATAGAAGACCAATTAAAGGTGGCAGGTGCCAAAAAAGGCGATGTCATTGTGTTTGCTGGCCAGGAATTTGTAATTAATGACTAA", "aa": "MLQADHNCDSLFFLKNKKHLFAESGGNGKPDLAHGKNGEDLVIKVPVGTTVRDLDTNQILMDFVHDQQSFILCYGGKGGKGNAAFKSPIMRAPNLYENGDKGQSLHVSLEIKYLANVGIVGFPNTGKSTLISKLSNAKPKIANYRFTTLVPVLGVVKHNDQSLVFADIPGLIENASEGSGLGHYFLRHIERCEILIHLISLDPVDHDDPCQAYEQIMRELSKYSQLLVKKKMLVVANKTDVDLDGTRFQKLAQYLENKGIPLFKISALKQELGDLVAQVFALHQKTLAQFGANKFHLPMEMEKHYVFEQASETDHDPLNIERDALGRWHVECKRLHYWFDKIPQTTLDNIRRLGNKIKEVGIEDQLKVAGAKKGDVIVFAGQEFVIND", "aa_hexdigest": "7c5bbd50a3cee0623c8e3dc979003ce8", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75215", "query_cov": 1.0, "subject_cov": 0.8960739030023095, "identity": 0.997, "score": 785.0, "evalue": 2.08e-286, "valid": true, "gene": "obgE", "product": "GTPase ObgE", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75215", "cog_id": "COG0536", "cog_category": "DL", "go_ids": [ "GO:0000287", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75215", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75215" ], "product": "GTPase Obg" }, "genes": [ "obgE" ], "id": "GIHJHMOALN_1195", "locus": "GCA009939745_01195" }, { "type": "cds", "sequence": "contig_1", "start": 679449, "stop": 679799, "strand": "-", "frame": 2, "gene": null, "product": "NADP-dependent alcohol dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A645H7Y1", "UniRef:UniRef90_A0AB38W5V0" ], "nt": "ATGGAAAAGGTGTTAATTTCTGGTGGTGGTTCGACTATCTTAGAAACAGCGATTAAGGTTTTAAGACCAGGAGGCAAAATTGGTAACGTTAATTACTTTGGCGCTGGTGAGTTTTTAACTATTCCTAGAGTTGAATGGGGCGTTGGTATGGCTCACAAGGCCATTCATGGTGGTCTTATGCTTGGTGGTCGGTTAAGGTTAGAAAAATTAGCACGTTTAATTATGACCAAAAAACTCGATCCTTCCAAAATGATTACCCATCGCTTTAAGGGTTTTGAACACATTGAAGAAGCCCTGTTTTTAATGAAAGATAAACCGAAGGACTTAATTAAATCCGTGGTTATTTTTTAA", "aa": "MEKVLISGGGSTILETAIKVLRPGGKIGNVNYFGAGEFLTIPRVEWGVGMAHKAIHGGLMLGGRLRLEKLARLIMTKKLDPSKMITHRFKGFEHIEEALFLMKDKPKDLIKSVVIF", "aa_hexdigest": "3dab30da03fae1a49e874498de88cc9e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002ED5893F", "query_cov": 0.9482758620689655, "subject_cov": 0.8148148148148148, "identity": 0.5820000000000001, "score": 139.0, "evalue": 1.88e-40, "valid": false, "uniref50_id": "UniRef50_A0A352UL42" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 679449, "stop": 680069, "observations": { "mutation": [ 679817, 680018, 680027 ], "stop": [ 679817, 680018, 680027 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W5V0", "query_cov": 1.0, "subject_cov": 0.5631067961165048, "identity": 1.0, "score": 230.0, "evalue": 2.22e-75, "gene_start": 1, "gene_end": 116, "reference_start": 91, "reference_end": 206, "reference_sequence": "MYVVRHDSHRLSRQWTSWHPIRCRVVVFGAGPVGLMAIAGAALKGAGRIIVVDSRPDIVEIAKAYGATDYIDFKKVSVVDEILKWTNNEGMEKVLISGGGSTILETAIKVLRPGGKIGNVNYFGAGEFLTIPRVEWGVGMAHKAIHGGLMLGGRLRLEKLARLIMTKKLDPSKMITHRFKGFEHIEEALFLMKDKPKDLIKSVVIF" }, "paralog": false, "identity": 0.9854368932038835, "score": 397.0, "evalue": 1.81e-143, "description": "Internal stop codon at 679817, 680018, 680027. Nonsense mutation around 679817, 680018, 680027.", "psc": { "uniref90_id": "UniRef90_A0AB38W5V0", "product": "NADP-dependent alcohol dehydrogenase", "uniref50_id": "UniRef50_A0A645H7Y1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W5V0", "UniRef:UniRef50_A0A645H7Y1" ] } }, "id": "GIHJHMOALN_1196", "locus": "GCA009939745_01196" }, { "type": "cds", "sequence": "contig_1", "start": 679815, "stop": 679964, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00107.32" ], "nt": "ATGGCGATCGCTGGTGCAGCCTTAAAGGGTGCTGGACGCATTATTGTGGTGGATTCTAGACCAGATATTGTGGAAATTGCCAAAGCATACGGTGCGACTGATTACATTGATTTCAAAAAGGTTTCAGTTGTGGATGAAATCTTGAAGTGA", "aa": "MAIAGAALKGAGRIIVVDSRPDIVEIAKAYGATDYIDFKKVSVVDEILK", "aa_hexdigest": "f8c5e57a2fa40ab36b66ca698a395dbf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00107.32", "name": "ADH_zinc_N", "length": 48, "aa_cov": 0.9795918367346939, "hmm_cov": 0.9791666666666666, "evalue": 1.6102888872629132e-09, "score": 33.29688262939453, "start": 1, "stop": 48 } ], "seq_stats": { "molecular_weight": 5192.0359, "isoelectric_point": 6.049965095520019 }, "id": "GIHJHMOALN_1197", "locus": "GCA009939745_01197" }, { "type": "cds", "sequence": "contig_1", "start": 679964, "stop": 680203, "strand": "-", "frame": 3, "gene": null, "product": "NADP-dependent alcohol dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B0EC62", "UniRef:UniRef90_A0AB38W6U1" ], "nt": "ATGCACTCCGGTGGCATGCTGGGTGGTTGGAAGTTTTCCAACGTTAAAGATGGCATGTTTGCTGAATACTTTCACGTCAATGATGCTGAGGGTAACTTAGCATTGATGCCAGAAGGGATGGATTTAGCTGATGCATGTATGTTGTCAGACATGATTCCCACAGGCTTTCACGCCAATGAACTAGCTGACATCCAATACGGTGTCGCGTTGTCGTTTTTGGTGCTGGTCCAGTTGGTTTAA", "aa": "MHSGGMLGGWKFSNVKDGMFAEYFHVNDAEGNLALMPEGMDLADACMLSDMIPTGFHANELADIQYGVALSFLVLVQLV", "aa_hexdigest": "c9b1a6cf83e7543b1ce617904b9f4554", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 679964, "stop": 680503, "observations": { "mutation": [ 680236, 680464 ], "stop": [ 680236, 680464 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W6U1", "query_cov": 1.0, "subject_cov": 0.441340782122905, "identity": 1.0, "score": 160.0, "evalue": 6.95e-49, "gene_start": 1, "gene_end": 79, "reference_start": 101, "reference_end": 179, "reference_sequence": "MKAYAMLKIGATGWIEKPRPVCGPNDAIIRPLAVAPCTSDVHTVWEGGIGERHNMVLGHEGCGVVDEVGSEVKSFKVGDRVLVAAITPEWNSVNAQAGYPMHSGGMLGGWKFSNVKDGMFAEYFHVNDAEGNLALMPEGMDLADACMLSDMIPTGFHANELADIQYGVALSFLVLVQLV" }, "paralog": false, "identity": 0.9888268156424581, "score": 361.0, "evalue": 2.43e-130, "description": "Internal stop codon at 680236, 680464. Nonsense mutation around 680236, 680464.", "psc": { "uniref90_id": "UniRef90_A0AB38W6U1", "product": "NADP-dependent alcohol dehydrogenase", "uniref50_id": "UniRef50_B0EC62", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W6U1", "UniRef:UniRef50_B0EC62" ] } }, "id": "GIHJHMOALN_1198", "locus": "GCA009939745_01198" }, { "type": "cds", "sequence": "contig_1", "start": 680234, "stop": 680341, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08240.17" ], "nt": "ATGGTTTTAGGCCATGAGGGCTGTGGTGTTGTGGATGAAGTGGGGTCTGAGGTCAAAAGCTTTAAGGTTGGTGATCGCGTTTTAGTGGCAGCCATTACACCAGAATGA", "aa": "MVLGHEGCGVVDEVGSEVKSFKVGDRVLVAAITPE", "aa_hexdigest": "028f5933663f10cf6e1ec59bc2a80dd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08240.17", "name": "ADH_N", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 5.13138026624124e-11, "score": 38.2827033996582, "start": 1, "stop": 32 } ], "seq_stats": { "molecular_weight": 3628.1351999999997, "isoelectric_point": 4.781830406188964 }, "id": "GIHJHMOALN_1199", "locus": "GCA009939745_01199" }, { "type": "cds", "sequence": "contig_1", "start": 680783, "stop": 680950, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGTGTTGGTAGTGTTAATGCTGAGTTTCTTTGTAATTAATAAACTAACTAACTTAGTTTTACTACGTTCACGCTATGCCATGGTAGTAGCACAAGGACTGGTTCACACAGGTAAAGTCAAACAACAAAGCCAACAGGCTATGTCTGACATTAAAACGAAATGA", "aa": "MLVLVVLMLSFFVINKLTNLVLLRSRYAMVVAQGLVHTGKVKQQSQQAMSDIKTK", "aa_hexdigest": "87bf19a1e80e57d93829103f5e0d3f66", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6190.500700000004, "isoelectric_point": 10.65547962188721 }, "id": "GIHJHMOALN_1200", "locus": "GCA009939745_01200" }, { "type": "cds", "sequence": "contig_1", "start": 680978, "stop": 681271, "strand": "-", "frame": 3, "gene": null, "product": "MPN565 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75213", "UniRef:UniRef90_P75213" ], "nt": "TTGGTCTTTGTCTACTACATCATTGTGTGGGTGTTATTAGGGGAAATTAATGCCTTAGGTTTTAACTTTGTCATTGCCCCAAATAACCGTACTTTAATGGGTAGGGAAGTAGAACCCATTTTTGATTTAGTACTACTGTGGTTCTTACTAGTGCCCTTAGTAGTTTATGCGTTGCTTTTACTGCTTTTATTCTTTACAACCCCATACCTAATTGTGGAGGCAATTCCCTTTTGTTATGGAATTGCTTTGATGATGATTAGCCTCTTTATGAGTGGTATGTTTCCTCAAGCTTGA", "aa": "MVFVYYIIVWVLLGEINALGFNFVIAPNNRTLMGREVEPIFDLVLLWFLLVPLVVYALLLLLLFFTTPYLIVEAIPFCYGIALMMISLFMSGMFPQA", "aa_hexdigest": "2661d68f16c021f36a56fa4daaceb78f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 680717, "stop": 681343, "observations": { "mutation": [ 680785, 680980, 681298 ], "stop": [ 680785, 680980, 681298 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75213", "query_cov": 1.0, "subject_cov": 0.46634615384615385, "identity": 0.9790000000000001, "score": 162.0, "evalue": 8.7e-49, "gene_start": 1, "gene_end": 97, "reference_start": 25, "reference_end": 121, "reference_sequence": "MEQFFQSTAKRKTPAWLTGSFSFFLVFVYYIIVWVLLGEINALGFNFVIAPNNRTLMGREVEPIFDLVLLWFLLVPLVVYALLLLLLFFTTPYLIVEAIPFFYGIALMMISLFMSGMFPQAWNVWVIFGRFVLVLVVLMLSFFVINKLTNLVLLRSRYAMVVAQGLVHTGKVKQQSQQAMSDIKTKWDKEKSKTVVVTIKKKRVKSSD" }, "paralog": false, "identity": 0.9759615384615384, "score": 373.0, "evalue": 3.66e-134, "description": "Internal stop codon at 680785, 680980, 681298. Nonsense mutation around 680785, 680980, 681298.", "psc": { "uniref90_id": "UniRef90_P75213", "product": "MPN565 family protein", "uniref50_id": "UniRef50_P75213", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75213", "UniRef:UniRef50_P75213", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75213", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75213" ], "product": "Uncharacterized protein MG384.1 homolog" } }, "id": "GIHJHMOALN_1201", "locus": "GCA009939745_01201" }, { "type": "cds", "sequence": "contig_1", "start": 681345, "stop": 682058, "strand": "-", "frame": 2, "gene": "ugpQ", "product": "putative MG385-like protein", "db_xrefs": [ "COG:COG0584", "COG:I", "GO:0006629", "GO:0008081", "RefSeq:WP_014325603.1", "SO:0001217", "UniParc:UPI0001DD6431", "UniRef:UniRef100_A0AAV5N9Y2", "UniRef:UniRef50_P47625", "UniRef:UniRef90_P75212" ], "nt": "ATGCGCAAACAGTTTTTAATTGCACACCGTGGTTACAGCACCATTGCTCCTGAAAACACTCATTTAGCCTTTGCTGCTGCACAATTGTTTGGTTTTGATGGTTTGTGGATGGAAGTGCAACTCACTAAAGATAAACAAATAGTAGTCACTAATTTAGACAACTATAAGGTAGGAAATAAAACCCATAAACTGAGTGATATTAACCTAGTTGATCTCAAAAAGATTAACTTAGCGAAGCAGTTTAAGGTAAATGTCCAAGAACAGACAATCTTGACCTTAAAAGAGGTGTTAATGGAATTTTTAACCCCGTTTCGCTTTCTCTTGCTTTTTATTAAGGGCGAAGAAGAACAACAAAACCAGGTACTAGTAGAACAGTTAGCACAACTACTAGAAGGTTTTGAGTTAGCTAAAGAAAAGTTAATCTTGCTGTCCAGTCACTTTTCCACGATTAAGTACCTCAACGAAAAGTTAAAGAGCTTTAAAACTAGTTTTGTCTTTAACAGTAAAAAGCAGTTAGTTCATTTAACTAAAGATGAGATTACCCAGAACTGTCGTTTCTTAGCGCCCAATGATAGCTTTTACCACAAAAATTGTGCGGAGCTACAAAGCTATGGCCTGCCAATTATCCTCTGGGTAATTAAAGGGCTTTTACGTTACCAGTTTTACGAAAACGATCGCTTTGTTAAATTTCAAATAGCAGCACAACTTTACTAA", "aa": "MRKQFLIAHRGYSTIAPENTHLAFAAAQLFGFDGLWMEVQLTKDKQIVVTNLDNYKVGNKTHKLSDINLVDLKKINLAKQFKVNVQEQTILTLKEVLMEFLTPFRFLLLFIKGEEEQQNQVLVEQLAQLLEGFELAKEKLILLSSHFSTIKYLNEKLKSFKTSFVFNSKKQLVHLTKDEITQNCRFLAPNDSFYHKNCAELQSYGLPIILWVIKGLLRYQFYENDRFVKFQIAAQLY", "aa_hexdigest": "aea43eab7ddd6825a501ca6f9e39b8ad", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6431", "ncbi_nrp_id": "WP_014325603.1", "uniref100_id": "UniRef100_A0AAV5N9Y2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6431", "RefSeq:WP_014325603.1", "UniRef:UniRef100_A0AAV5N9Y2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N9Y2", "uniref90_id": "UniRef90_P75212" }, "psc": { "uniref90_id": "UniRef90_P75212", "gene": "UgpQ", "product": "Uncharacterized protein MG385 homolog", "uniref50_id": "UniRef50_P47625", "cog_id": "COG0584", "cog_category": "I", "go_ids": [ "GO:0008081", "GO:0006629" ] }, "pscc": { "uniref50_id": "UniRef50_P47625", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47625" ], "product": "Uncharacterized protein MG385" }, "genes": [ "ugpQ" ], "id": "GIHJHMOALN_1202", "locus": "GCA009939745_01202" }, { "type": "cds", "sequence": "contig_1", "start": 682454, "stop": 682570, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCATTATGAACCTAGTGGGGAATGGGTATGAGCTGGTTACTTTGATGACAACCAAATATGAACACCAGATGCTAGTGTTGAATGGGCACGTGAAAGTGATTACACAGATCTAA", "aa": "MVIMNLVGNGYELVTLMTTKYEHQMLVLNGHVKVITQI", "aa_hexdigest": "8f59a77be459679adb3303dbbee94c4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4332.179400000001, "isoelectric_point": 6.696679115295409 }, "id": "GIHJHMOALN_1203", "locus": "GCA009939745_01203" }, { "type": "cds", "sequence": "contig_1", "start": 682596, "stop": 683000, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAACGATTCCTGAACCAGAATCAATTGAAACTACTGAACCAGAACCTAATTTTGAACCAGAAGTAGAGCTTGAACCAGAAATTGAACCTAATTTCGAATCCGAAACTGAAGTTCAACAAGAGCTAGCACAAGAATCAAGTTTCGAGTCTGAACCAGAACCTAATTTTGAAACAGAAGTAGAAGTTCAACCTGAATCAGAAATTGAATTTAAATTCGAAGCCGAAGTTCAATCAGAGCCAAAAGTAAGTTTGAATTCAGATTTTGAAACTAAACCAGAAGCTCAAGCGGAGGTAACGCCAGAAACCTTGGAAGTAGAGGCAACATCTGAAGCTCCAGAACTTCAACCAGAAACTGAAGCAACAAAAGTAGTTGATGACGTTGAAGAAGAGCAACTTGATTGA", "aa": "METIPEPESIETTEPEPNFEPEVELEPEIEPNFESETEVQQELAQESSFESEPEPNFETEVEVQPESEIEFKFEAEVQSEPKVSLNSDFETKPEAQAEVTPETLEVEATSEAPELQPETEATKVVDDVEEEQLD", "aa_hexdigest": "c1d14925fd733753e610126553aa4a74", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15215.904899999992, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_1204", "locus": "GCA009939745_01204" }, { "type": "cds", "sequence": "contig_1", "start": 683178, "stop": 684425, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16713.10" ], "nt": "GTGTGGAAGGGTTTCTTTGACACCAAACTCAATTGGATTCCTGACCCAGTGCGCTTTACCTTGCCAATGGTTGAAAAAGCCACTACAACTACACCTGTTGTACAAATTGAATTACCAGCACCACCAACTGTAACTGTAGTAGACCAAACATCACCACCAACTGCTGCTGTCACCGTTTCTACATCACAGCCAGTCATAGAAGAGCAGACCACTGTATTTAATCAAACTACTCAGCTAGAACAGCTAAGTGTTAGCGCACCACTGTTAGACCAATCGGAAGTGGAAACGGAAATGGTAGAAGTGCCGTTTGTTGCACCGTCAACAACTACCACTCAACCTCAAGTGGTTACTGTACAAGCACAACCAGCATCAAGTTCGATTCAGTTTCAAGAACCAATTATTAAGGTTGAGTTTGTTAATGAAAGTTTTGATTTCAAAAAACCAAGCCAAACAGCAGCTGCAGCTAGTCAAGCCCCTTCTCAAGCAATTAACATTGCTTTAAATGAAGCTGACCTCATTGATGAATTAGTGGCGGTTGGCACCACAGCCACCACAGCCTTACCACAAAGCGAGCTGATTCAAGAGGTGGTGGTGATTGACAACGGTCAACCCCAACAAGCTGGGTTTCATTATGTAGTTGATTTTCTCACTAGCACTGCACCACTAACTGTAGCAGAAATAGAGTTGCAAGAGCAAGAGCTAGTCAATGAGTTTGTCACAACTACTAGTAGAGAAACAACTACTTTTGCATCAACTCCAGTTTTTGAACCAGTTGTAATTCCAACAGTAGAACCTGAAGAACAACTTTTAGAAAACGAGTTTGTTGAATCAACTGTTGTAAGTGCAACATCAAATGAACCTAATGTTGCATCAACTCCAGTTGTTGAAACGGTTGAACTTACAGAAACACCAGTAAGTTTAGAACCGCTTGAAACTGTTCAACTAGAAACAGCACCAGTTGTTACTGAAACTGTTACAGTTACTGAGAAAGCAGTAGAACCAGAAGTATTAGCAGTTGTTGAAGAAGCACCACTTGCTGTAGAACCAATTGTTGAAACTTCAACAACATTAGCTGCAGAGACCGTTGAAGAAGCGCAAGTTGAACAAGAGTCAACAGCTGTTGCAGTCGAACCAGCTATTGAAACCGAGTCAAAAGCAACTTCTGAAGCCCAAGCTGAACTGGATTGGGAGGCGTTAATTGGTAACAGTGAATACGGTTACTTTGATGCAGAGCAAAACTGGATTTGA", "aa": "MWKGFFDTKLNWIPDPVRFTLPMVEKATTTTPVVQIELPAPPTVTVVDQTSPPTAAVTVSTSQPVIEEQTTVFNQTTQLEQLSVSAPLLDQSEVETEMVEVPFVAPSTTTTQPQVVTVQAQPASSSIQFQEPIIKVEFVNESFDFKKPSQTAAAASQAPSQAINIALNEADLIDELVAVGTTATTALPQSELIQEVVVIDNGQPQQAGFHYVVDFLTSTAPLTVAEIELQEQELVNEFVTTTSRETTTFASTPVFEPVVIPTVEPEEQLLENEFVESTVVSATSNEPNVASTPVVETVELTETPVSLEPLETVQLETAPVVTETVTVTEKAVEPEVLAVVEEAPLAVEPIVETSTTLAAETVEEAQVEQESTAVAVEPAIETESKATSEAQAELDWEALIGNSEYGYFDAEQNWI", "aa_hexdigest": "ac3b7393163d434d5d3ea1605359081a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16713.10", "name": "EAGR_box", "length": 21, "aa_cov": 0.05060240963855422, "hmm_cov": 1.0, "evalue": 2.267200034251852e-11, "score": 38.74320983886719, "start": 395, "stop": 415 } ], "seq_stats": { "molecular_weight": 44802.23660000011, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_1205", "locus": "GCA009939745_01205" }, { "type": "cds", "sequence": "contig_1", "start": 684651, "stop": 685172, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAAACGATAAAAAAACAGAACCCATCGAACACTACATTACAGTACAAAAAGTATTTAGAACAGTCTAAAGAGAAAACTGCTAAAGCTAAAAATAAGGATGTTAGCATCGATGATTTACTCAAGAAGCCCTTCTTAGAGGAGATCAAAACCAATGTACTTAAGAAGAATAAAACTACCAGAGCTAGCACCGCTACCAGAGGTACTTCTAAAGTCAAAAAACAGATAGTTGAATCTTCTATTGACTTTTTTGACGAGAAAAAGCGTGGTGTTTTTATTGTGCCACCAGCTGGTACTAGTGTTATTAACGATGACCGGGGTGATAATAAAGCGGTAGAGGAGACTGTTAGCAAAACTGCTATTAGTCAAAATCAACTGGCACATTATGCCAACAGTGAGCTTGTTGAAACCGAACAATTTGAACTAAAACCGGTTGCTTTAGAACAGAATCAGGTTCTAACCAGCACCCGCCACAGTCAAGAACGTGAATCGATTTTTGAAAAAGCACAACTCTTTTGA", "aa": "MPKTIKKQNPSNTTLQYKKYLEQSKEKTAKAKNKDVSIDDLLKKPFLEEIKTNVLKKNKTTRASTATRGTSKVKKQIVESSIDFFDEKKRGVFIVPPAGTSVINDDRGDNKAVEETVSKTAISQNQLAHYANSELVETEQFELKPVALEQNQVLTSTRHSQERESIFEKAQLF", "aa_hexdigest": "a28868e9811f6e20fe891d751d4025fa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19616.997699999996, "isoelectric_point": 9.43464183807373 }, "id": "GIHJHMOALN_1206", "locus": "GCA009939745_01206" }, { "type": "cds", "sequence": "contig_1", "start": 685272, "stop": 685652, "strand": "-", "frame": 2, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "EC:3.6.5.-", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_P47627", "UniRef:UniRef90_P75210" ], "nt": "GTGAAACTACAAGCAAATGAGTTTCGTAAAGATGTTGAATTTTTAGATGCTGACACTGATAACTTTAAGATTTTAGAAGCGCTGCGCGAACAGATCATTAAGTACTGCAGCGAAGAAATTCCCCATGTAGTAAGGCTAGAGATTGTCGATAAGAGTTTTGATCAAACTAAAAACCTGTTGAAGCTACACCTTTCCATTAGTGTGCCTAAACTTAGTCAAAAAAAAATTATTATTGGCCGTAATGCCCAAATGGTGAAAAAGATCGGGATGCAAATGCGTCAAAAACTACTGGAGTACTATGACTGCAATGTCTTTGTGGAATTGTTTGTAAGGACTTATGACCCTAAACAAAAAAAGTCACCTTATGGCTCTTTAATTTAG", "aa": "MKLQANEFRKDVEFLDADTDNFKILEALREQIIKYCSEEIPHVVRLEIVDKSFDQTKNLLKLHLSISVPKLSQKKIIIGRNAQMVKKIGMQMRQKLLEYYDCNVFVELFVRTYDPKQKKSPYGSLI", "aa_hexdigest": "26ac3fc57838263fdb10097796a3177f", "start_type": "GTG", "rbs_motif": null, "genes": [ "era" ], "truncated": "3-prime", "pseudogene": { "start": 685272, "stop": 685952, "observations": { "mutation": [ 685670 ], "stop": [ 685670 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75210", "query_cov": 1.0, "subject_cov": 0.4329896907216495, "identity": 0.9840000000000001, "score": 244.0, "evalue": 1.23e-79, "gene_start": 1, "gene_end": 126, "reference_start": 166, "reference_end": 291, "reference_sequence": "MESIRIGVLGLTNAGKSTLVNQLHKANNLLVSPMNNTTLLAVSTNTITHEKQNITFIDVPGFSEKRHSSYELISQEIRKALSGIDVLLLVVRSDQQQKLPLLQTQLQPLKRYRDLTRVLLINNFFDVVLQAEDKQAIVLDFKPQAVLETDLLHFDATSFWNQFNEVKLQANEFRKDVEFLDADTDNFKILEALREQIIKYCSEEIPHVVRLEIVDKSFDQAKNLLKLHLSISVPKLSQKKIIIGRNAQMVKKIGMQMRQKLLEYYDCNVFVELFVRTYDPKQKKSPYGSLI" }, "paralog": false, "identity": 0.9911504424778761, "score": 434.0, "evalue": 9.67e-157, "description": "Internal stop codon at 685670. Nonsense mutation around 685670.", "psc": { "uniref90_id": "UniRef90_P75210", "gene": "era", "product": "GTPase Era", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P47627", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75210", "EC:3.6.5.-", "UniRef:UniRef50_P47627", "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47627", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47627" ], "product": "GTPase Era" } }, "id": "GIHJHMOALN_1207", "locus": "GCA009939745_01207" }, { "type": "cds", "sequence": "contig_1", "start": 685668, "stop": 686147, "strand": "-", "frame": 2, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "EC:3.6.5.-", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_P47627", "UniRef:UniRef90_P75210" ], "nt": "ATGGAATCGATAAGAATTGGTGTTTTAGGTCTAACCAACGCTGGTAAATCAACTCTCGTTAACCAATTACACAAAGCGAATAACTTGTTGGTTTCACCTATGAACAACACTACGTTGTTGGCGGTTAGCACTAATACTATTACCCATGAAAAGCAAAACATTACCTTCATTGATGTTCCTGGCTTTAGTGAAAAACGTCACAGCTCCTATGAGTTAATTAGTCAAGAGATTCGCAAGGCTTTAAGTGGGATAGATGTCTTGTTGTTAGTGGTGCGCAGTGATCAACAGCAAAAGTTACCCTTGTTACAAACGCAATTACAACCGTTAAAGCGTTACCGTGATTTAACTCGTGTTTTGTTAATTAATAACTTCTTTGATGTTGTTTTGCAAGCGGAGGACAAGCAAGCAATTGTGCTCGATTTTAAACCGCAAGCAGTACTGGAAACTGATCTGCTTCATTTTGATGCCACTTCTTTTTGA", "aa": "MESIRIGVLGLTNAGKSTLVNQLHKANNLLVSPMNNTTLLAVSTNTITHEKQNITFIDVPGFSEKRHSSYELISQEIRKALSGIDVLLLVVRSDQQQKLPLLQTQLQPLKRYRDLTRVLLINNFFDVVLQAEDKQAIVLDFKPQAVLETDLLHFDATSF", "aa_hexdigest": "dfe2d35fec5a8946cc6e9fd10130e6a3", "start_type": "ATG", "rbs_motif": null, "genes": [ "era" ], "truncated": "5-prime", "pseudogene": { "start": 685365, "stop": 686147, "observations": { "mutation": [ 685670 ], "stop": [ 685670 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75210", "query_cov": 1.0, "subject_cov": 0.5463917525773195, "identity": 1.0, "score": 297.0, "evalue": 7.31e-100, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MESIRIGVLGLTNAGKSTLVNQLHKANNLLVSPMNNTTLLAVSTNTITHEKQNITFIDVPGFSEKRHSSYELISQEIRKALSGIDVLLLVVRSDQQQKLPLLQTQLQPLKRYRDLTRVLLINNFFDVVLQAEDKQAIVLDFKPQAVLETDLLHFDATSFWNQFNEVKLQANEFRKDVEFLDADTDNFKILEALREQIIKYCSEEIPHVVRLEIVDKSFDQAKNLLKLHLSISVPKLSQKKIIIGRNAQMVKKIGMQMRQKLLEYYDCNVFVELFVRTYDPKQKKSPYGSLI" }, "paralog": false, "identity": 0.9923076923076923, "score": 495.0, "evalue": 4.42e-180, "description": "Internal stop codon at 685670. Nonsense mutation around 685670.", "psc": { "uniref90_id": "UniRef90_P75210", "gene": "era", "product": "GTPase Era", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P47627", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75210", "EC:3.6.5.-", "UniRef:UniRef50_P47627", "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47627", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47627" ], "product": "GTPase Era" } }, "id": "GIHJHMOALN_1208", "locus": "GCA009939745_01208" }, { "type": "cds", "sequence": "contig_1", "start": 686135, "stop": 686440, "strand": "-", "frame": 3, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "EC:3.1.-.-", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47628", "UniRef:UniRef90_P75209" ], "nt": "ATGAAGCGCTTAAACCGGCAGTTTCTCAACCGCAAAGGGGTAACTGATGTGATTAGCATCTGTTACAACGAAAATGAGGCGGGGTTTTCACCAGCCATTGGTGAAATCTTTTTGTGTCCCAAACACATCTTTAAGCAGGCCAAGCAGTTTGGTTGCACACCTTGGTTTTTACTAACACGCAACTTAATCCATGGTTTGCTACACCTGTTTGAGTTTGACCATGAGCAAAGTCTGGCCTTTGAAAGTGTAACGATGTTTTTTCAAGATGAAATTCATGAAACCGTTTTAAAACTATGGAATCGATAA", "aa": "MKRLNRQFLNRKGVTDVISICYNENEAGFSPAIGEIFLCPKHIFKQAKQFGCTPWFLLTRNLIHGLLHLFEFDHEQSLAFESVTMFFQDEIHETVLKLWNR", "aa_hexdigest": "81f26937fc4e70cc3ffaf4e7419feb3e", "start_type": "ATG", "rbs_motif": null, "genes": [ "ybeY" ], "truncated": "3-prime", "pseudogene": { "start": 686135, "stop": 686599, "observations": { "mutation": [ 686446 ], "stop": [ 686446 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75209", "query_cov": 1.0, "subject_cov": 0.6558441558441559, "identity": 1.0, "score": 213.0, "evalue": 1.08e-69, "gene_start": 1, "gene_end": 101, "reference_start": 54, "reference_end": 154, "reference_sequence": "MKPSFSINSNYLFKRFFGKNFEAGIELCLQIIKDSLQLSFQPEFALMIVSPWKMKRLNRQFLNRKGVTDVISICYNENEAGFSPAIGEIFLCPKHIFKQAKQFGCTPWFLLTRNLIHGLLHLFEFDHEQSLAFESVTMFFQDEIHETVLKLWNR" }, "paralog": false, "identity": 0.987012987012987, "score": 314.0, "evalue": 1.21e-111, "description": "Internal stop codon at 686446. Nonsense mutation around 686446.", "psc": { "uniref90_id": "UniRef90_P75209", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47628", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75209", "EC:3.1.-.-", "UniRef:UniRef50_P47628", "COG:COG0319", "COG:J", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47628", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47628" ], "product": "Endoribonuclease YbeY" } }, "id": "GIHJHMOALN_1209", "locus": "GCA009939745_01209" }, { "type": "cds", "sequence": "contig_1", "start": 686787, "stop": 686972, "strand": "-", "frame": 2, "gene": null, "product": "putative MG389-like protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47629", "UniRef:UniRef90_P75208" ], "nt": "ATGAAGCAAAAAATTATTGGCCTAACTCTAGCTTTCTTTGTTTTATTCTTAACAGCCGTTGCCATCTTATTTACGGTTAAGGTACAACGATACCTAACTACAAGCTTATGGGCAAAATTGTCTGAACAAACCTTTTTAGTTTTTAAAAACGAGCAGGATGAAGCCCAGTTTAACCAGGTATCTTGA", "aa": "MKQKIIGLTLAFFVLFLTAVAILFTVKVQRYLTTSLWAKLSEQTFLVFKNEQDEAQFNQVS", "aa_hexdigest": "fbf65018700e9ab47c995fe43f42d65b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 686583, "stop": 686972, "observations": { "mutation": [ 686789 ], "stop": [ 686789 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75208", "query_cov": 1.0, "subject_cov": 0.4728682170542636, "identity": 1.0, "score": 112.0, "evalue": 1.06e-30, "gene_start": 1, "gene_end": 61, "reference_start": 1, "reference_end": 61, "reference_sequence": "MKQKIIGLTLAFFVLFLTAVAILFTVKVQRYLTTSLWAKLSEQTFLVFKNEQDEAQFNQVSWTNFQAETTKKEDKKAFRLYKKKISLEQLENENQQQLFQAVNLSINLKQGWYNITILLPSKALFETVF" }, "paralog": false, "identity": 0.9922480620155039, "score": 248.0, "evalue": 1.7e-86, "description": "Internal stop codon at 686789. Nonsense mutation around 686789.", "psc": { "uniref90_id": "UniRef90_P75208", "product": "Uncharacterized protein MG389 homolog", "uniref50_id": "UniRef50_P47629", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75208", "UniRef:UniRef50_P47629", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47629", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47629" ], "product": "Uncharacterized protein MG389" } }, "id": "GIHJHMOALN_1210", "locus": "GCA009939745_01210" }, { "type": "cds", "sequence": "contig_1", "start": 686974, "stop": 687282, "strand": "-", "frame": 1, "gene": null, "product": "ABC-type enterochelin transport system, ATPase component", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002E79EA9", "UniRef:UniRef100_A0AAV5N885", "UniRef:UniRef50_A0AAV5N885", "UniRef:UniRef90_A0AAV5N885" ], "nt": "TTGTTAAACTGTAAAAATCCCCAAGTATACCAGCTGTTAGCCGACACCGGCATCCACAACCAGATTAAGTTAAGTTCAGGACAAAAACAGATCTTGCAACTGTTCTTATTACAAAACCTCAAAGACAAGGTGATTTTGTTAGATGAAACACTCAACGCCATTGCCACGGAGCTCAAACCGCGCGTGTATCAGCTTTTGATCAAACCGTTGACCTATAACAACTTTGTTCTGATGGTCGAACACGATTTACGCTTTGTAAACAGTGAGCAAGACCTAATTAACCTCAGTCCTTATTTACAGCAGACATAA", "aa": "MLNCKNPQVYQLLADTGIHNQIKLSSGQKQILQLFLLQNLKDKVILLDETLNAIATELKPRVYQLLIKPLTYNNFVLMVEHDLRFVNSEQDLINLSPYLQQT", "aa_hexdigest": "d1a60becec6b7c5bc540933862ca058b", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002E79EA9", "uniref100_id": "UniRef100_A0AAV5N885", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002E79EA9", "UniRef:UniRef100_A0AAV5N885" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N885", "uniref90_id": "UniRef90_A0AAV5N885" }, "psc": { "uniref90_id": "UniRef90_A0AAV5N885", "product": "ABC-type enterochelin transport system, ATPase component", "uniref50_id": "UniRef50_A0AAV5N885" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N885", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N885" ], "product": "ABC-type enterochelin transport system, ATPase component" }, "genes": [], "id": "GIHJHMOALN_1211", "locus": "GCA009939745_01211" }, { "type": "cds", "sequence": "contig_1", "start": 687276, "stop": 688268, "strand": "-", "frame": 2, "gene": "sunT", "product": "Putative ABC transporter ATP-binding MG390-like protein", "db_xrefs": [ "COG:COG2274", "COG:V", "GO:0005524", "GO:0006508", "GO:0008234", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P75207", "UniRef:UniRef90_P75207" ], "nt": "ATGCAAACATTACAAACCAAGAGTTTTGTCTACTTTAGCAATAAATCACCTAACCAATTAACCGAGCGTCAGTTTTACCTCAAGGAAGTGTTGTCCTTCTTTAATGTCCATATTCCTAACTTAATTATTAGCTGTACAGTAGCTTTAATTATTGGCACCTTAATTGGTATTAACCAAATGGAGTTCTTATGGATAGCAATTGCCCAAATTGTGGTGAATTGTGCCATTTTTCTTTATGACTTCTTTTTCACTAAACGGATTACGAAGCAAGCAATTCCGCAAATGGAGTTACAAAACAAGGTCAGTTTGCAGTTAGATGGCAATTTGCGTGATGAACAAAACGGCAAGCGCTTCAATTACTTAATGATGCAGTTGCGCAAAGCACTTATTAAAAACCAAAACATTAGTAATCAAAAGGAAGTGAACCACTTAGCGAGTGATGGTGTTAAAAGTTTTGCCCAACAGGTATTTGACTTTTTAATTTTGGCCTTGGGCATTATTGGCATTATTGAACAGCGCTACACCTTAGCCTTTCTCTTTTATATCTTTTCCATTCAGGCATTGTTTTCCGCTTATGCCACGAGAATCATCCAGTTTGGTGCAGCAGTTAACCTGTACCAGTTCTGTAAGGACAAACTAGTTACCTTGTTTGAAGACAAGGTTAATGATTGCAATTTCAAGGTCAGTTGGAAGTGTCCCAAAGTGATTAACTTAAACAACTGTAGTATCACTCTGAACCAAAATCTCGATTTGGCTAACCTCAACCTGAATTTAACTAATGGCATGGTTATTAGTGGTGAGAATGGTAGCGGTAAAAGTACCTTGCTGAAAATTCTGACTGGTCGAGGTTTGTCATACCAGGGTCAAATTAAGTTGGATGAATTAGACCTAAAGGATTTTAGTGCAAGCCAACTGTTTCACAACGTTTACTATTTAACGGGGCAGTTAACGGCCTACAACGACATTACTGACTTTACAGTGAAGCCTTGTTAA", "aa": "MQTLQTKSFVYFSNKSPNQLTERQFYLKEVLSFFNVHIPNLIISCTVALIIGTLIGINQMEFLWIAIAQIVVNCAIFLYDFFFTKRITKQAIPQMELQNKVSLQLDGNLRDEQNGKRFNYLMMQLRKALIKNQNISNQKEVNHLASDGVKSFAQQVFDFLILALGIIGIIEQRYTLAFLFYIFSIQALFSAYATRIIQFGAAVNLYQFCKDKLVTLFEDKVNDCNFKVSWKCPKVINLNNCSITLNQNLDLANLNLNLTNGMVISGENGSGKSTLLKILTGRGLSYQGQIKLDELDLKDFSASQLFHNVYYLTGQLTAYNDITDFTVKPC", "aa_hexdigest": "2cc1c18ca920706a12ef1485b2151151", "start_type": "ATG", "rbs_motif": null, "genes": [ "sunT" ], "truncated": "both", "pseudogene": { "start": 686974, "stop": 688568, "observations": { "deletion": [ 687293 ], "mutation": [ 688274, 688301 ], "stop": [ 688274, 688301 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75207", "query_cov": 0.9848484848484849, "subject_cov": 0.49242424242424243, "identity": 0.997, "score": 621.0, "evalue": 3.32e-219, "gene_start": 1, "gene_end": 325, "reference_start": 229, "reference_end": 553, "reference_sequence": "MKIIYQEQPNECGICVLGMLANELHEDKYAHDELLEQINLPASGLSFFELETYGKKFGLEIASYQLTLEELKQLEGKYFIVHFPKHFVVVHKKQDNLWEVFDPAKGKYLLNDEELKKQWTGYAATVQKSFKEIPPINKRNFFKHFFDLNLIIFYVFIELIIIGISTLLATASKTMIANTVDFGTSVNIVVFVVFFLVLKGLYLLLYALLQMVRNVLFWKQYRGYLGWIMQTLQTKSFVYFSNKSPNQLTERQFYLKEVLSFFNVHIPNLIISCTVALIIGTLIGINQMEFLWIAIVQIVVNCAIFLYDFFFTKRITKQAIPQMELQNKVSLQLDGNLRDEQNGKRFNYLMMQLRKALIKNQNISNQKEVNHLASDGVKSFAQQVFDFLILALGIIGIIEQRYTLAFLFYIFSIQALFSAYATRIIQFGAAVNLYQFCKDKLVTLFEDKVNDCNFKVSWKCPKVINLNNCSITLNQNLDLANLNLNLTNGMVISGENGSGKSTLLKILTGRGLSYQGQIKLDELDLKDFSASQLFHNVYYLTGQLTAYNDITDFGYSEALLNCKNPQVYQLLADTGIHNQIKLSSGQKQILQLFLLQNLKDKVILLDETLNAIATELKPRVYQLLIKPLTYNNFVLMVEHDLRFVNSEQDLINLSPYLQQT" }, "paralog": false, "identity": 0.9924812030075187, "score": 1021.0, "evalue": 0.0, "description": "Internal stop codon at 688274, 688301. Frameshift due to deletion around 687293. Nonsense mutation around 688274, 688301.", "psc": { "uniref90_id": "UniRef90_P75207", "gene": "SunT", "product": "Putative ABC transporter ATP-binding MG390 homolog", "uniref50_id": "UniRef50_P75207", "cog_id": "COG2274", "cog_category": "V", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0008234", "GO:0006508" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75207", "UniRef:UniRef50_P75207", "COG:COG2274", "COG:V", "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0008234", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P75207", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75207" ], "product": "Putative ABC transporter ATP-binding MG390 homolog" } }, "id": "GIHJHMOALN_1212", "locus": "GCA009939745_01212" }, { "type": "cds", "sequence": "contig_1", "start": 688299, "stop": 688577, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAAATCATTTAAAGAAATTCCGCCCATTAATAAGCGCAATTTCTTTAAGCACTTCTTTGATTTAAACCTGATTATTTTCTATGTCTTCATTGAACTAATCATTATTGGCATTTCAACACTTTTAGCAACGGCCTCTAAAACGATGATTGCCAACACCGTTGACTTTGGGACATCAGTTAACATTGTCGTTTTTGTGGTTTTCTTTTTAGTTTTAAAAGGCTTGTACTTACTTTTGTATGCCTTGTTGCAAATGGTGCGTAATGTTTTGTTTTGA", "aa": "MQKSFKEIPPINKRNFFKHFFDLNLIIFYVFIELIIIGISTLLATASKTMIANTVDFGTSVNIVVFVVFFLVLKGLYLLLYALLQMVRNVLF", "aa_hexdigest": "7666c791d5f5341b6690e0381e40269d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10638.804399999994, "isoelectric_point": 9.69728603363037 }, "id": "GIHJHMOALN_1213", "locus": "GCA009939745_01213" }, { "type": "cds", "sequence": "contig_1", "start": 688596, "stop": 688952, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTATTTATCAAGAACAACCCAACGAATGTGGCATTTGTGTGCTTGGCATGTTAGCCAATGAACTGCATGAGGATAAGTATGCCCATGATGAACTGTTGGAACAAATTAACCTACCAGCTAGTGGTTTGAGTTTCTTTGAACTAGAGACCTATGGCAAGAAGTTTGGTCTCGAAATTGCTAGTTACCAGCTAACCCTAGAAGAGCTCAAACAACTAGAGGGTAAATACTTTATTGTCCACTTCCCTAAGCATTTTGTGGTGGTGCATAAAAAACAAGATAATCTCTGGGAAGTATTTGACCCCGCTAAAGGGAAATATCTTTTAAATGATGAAGAATTAAAGAAACAGTGA", "aa": "MKIIYQEQPNECGICVLGMLANELHEDKYAHDELLEQINLPASGLSFFELETYGKKFGLEIASYQLTLEELKQLEGKYFIVHFPKHFVVVHKKQDNLWEVFDPAKGKYLLNDEELKKQ", "aa_hexdigest": "ce8098bad00b7f2b0d6cabca416d3d6c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13817.719499999987, "isoelectric_point": 5.183454704284666 }, "id": "GIHJHMOALN_1214", "locus": "GCA009939745_01214" }, { "type": "cds", "sequence": "contig_1", "start": 689056, "stop": 689286, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCACCCGGATTACCTAAAACCATTGGAATCAAAACTAGCGGACTTACAAAACTCCACTAGTGTTAAGGGAGCAGGTTCATCCCGGGCGGCATGTTTCTTAGCTGAATTTAGGGAAGGGGTACCTTTGATCCACTGTGATATTGCATCCACAGCTTCGATTCAAGATCTTGGTCAAGGGGTTTTAGTACGTACTTTATACGAACGTGCTGCGCAGCAAGCAAAGGAATAA", "aa": "MHPDYLKPLESKLADLQNSTSVKGAGSSRAACFLAEFREGVPLIHCDIASTASIQDLGQGVLVRTLYERAAQQAKE", "aa_hexdigest": "4fbf813e5ddb1cf735b4bca6149ef76a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 73, "aa_cov": 0.8552631578947368, "hmm_cov": 0.9863013698630136, "evalue": 4.090068262624576e-12, "score": 41.44631576538086, "start": 2, "stop": 66 } ], "seq_stats": { "molecular_weight": 8203.218999999997, "isoelectric_point": 6.064061164855956 }, "id": "GIHJHMOALN_1215", "locus": "GCA009939745_01215" }, { "type": "cds", "sequence": "contig_1", "start": 689296, "stop": 690393, "strand": "-", "frame": 1, "gene": "pepA", "product": "leucyl aminopeptidase", "db_xrefs": [ "COG:COG0260", "COG:E", "EC:3.4.11.1", "GO:0005737", "GO:0006508", "GO:0030145", "GO:0070006", "SO:0001217", "UniRef:UniRef50_P47631", "UniRef:UniRef90_P75206" ], "nt": "ATGAAATTAAATAAGTTATTTGACAGCAAGACGGTTGTTTTTAAAAAGTCCGATAAGTACGGCAAGAGTGATTGCTGTAAGACTAAGTGTGTTGAAGGCCAAATTGAATTTGGCGTAAAAATTCCGACTGATTTCCCCGCTTTCAACCGCGCTCTAGTTACCTTTTTGAAAACGCAAAAGAACCAACTAAACGTGGACTTAGACAGTTTTGTGGAACTCTACAAAGCAGAAAACCTCTGTTACAAAACAGCATTAAAGGTAGTGGTAGCTAGCATTACCTTCTGTGAAACAACACCTTTCACCATGAAGACCGAACCACAAAAGAATGTGGAAGTAGCAGTGAAGTGTGACAGCGAACACACGAGCCTCATTAAAGAATACGAGGTAGTGGGTAACTATGTCAACATGGCGCGTCAACTCCAAGACACCCCCTCTGACCAACTCTACCCAGAGGAATTTGTGAAGCGCTTTGAAAAGGCGGCTACTGGTTTAGGTGTTAAGATTACCGTTTTAAAGCAAGCTGATCTCATCAAAAAGAAGATGGGCTTACTGTTAGGGGTTAACAAGGGTTCGGAACGTGAAGCGCGTTTACTAGTAATTTCCTATAACAATAATAAGAAGAGCAGTGAAACTTTAGCTTTAGTTGGTAAGGGCATTACCTATGATTCGGGTGGGATGAACATTAAGACCGGTGACTACATGCGTGGTATGAAGTACGACATGAGTGGGGCAGCTATTGTGTGTTCCACCGTGTTGGCCTTAGCGAAAAACAAGGTGAAAACCAATGTAGTAGCAGTAGCTGCTTTAACAGAAAACCTACCAGGTCCCCACGCACAACGCCCTGATGACATCCAAACCGCTTACAATGGTAAAACGGTTGAAATTGACAATACCGATGCTGAAGGTCGCTTGGTGTTAGCCGATGCCATTAGCTATGCTGCTAAGGATCTTAAAGCTACCCAAATTATTGATGTCGCTACCTTAACCGGTTTAATGTCTTACATCTTAAGTACCACTTACACCGGGATCTTTAGTACCTGTGACATGGCATGGGATGCCTTTAAAAAAGCAGCTTGCTGTGCAGGCGAACCGGTATGA", "aa": "MKLNKLFDSKTVVFKKSDKYGKSDCCKTKCVEGQIEFGVKIPTDFPAFNRALVTFLKTQKNQLNVDLDSFVELYKAENLCYKTALKVVVASITFCETTPFTMKTEPQKNVEVAVKCDSEHTSLIKEYEVVGNYVNMARQLQDTPSDQLYPEEFVKRFEKAATGLGVKITVLKQADLIKKKMGLLLGVNKGSEREARLLVISYNNNKKSSETLALVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGPHAQRPDDIQTAYNGKTVEIDNTDAEGRLVLADAISYAAKDLKATQIIDVATLTGLMSYILSTTYTGIFSTCDMAWDAFKKAACCAGEPV", "aa_hexdigest": "fdd1ae83e24b3cd833bd1a60de804876", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75206", "query_cov": 1.0, "subject_cov": 0.8202247191011236, "identity": 1.0, "score": 709.0, "evalue": 9.43e-257, "valid": true, "gene": "pepA", "product": "leucyl aminopeptidase", "ec_ids": [ "3.4.11.1" ], "uniref50_id": "UniRef50_P47631", "cog_id": "COG0260", "cog_category": "E", "go_ids": [ "GO:0005737", "GO:0030145", "GO:0070006", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47631", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47631" ], "product": "Probable cytosol aminopeptidase" }, "genes": [ "pepA" ], "id": "GIHJHMOALN_1216", "locus": "GCA009939745_01216" }, { "type": "cds", "sequence": "contig_1", "start": 690407, "stop": 692038, "strand": "-", "frame": 3, "gene": "groL", "product": "chaperonin GroEL", "db_xrefs": [ "COG:COG0459", "COG:O", "EC:5.6.1.7", "GO:0005524", "GO:0005737", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0140662", "RefSeq:WP_010874930.1", "SO:0001217", "UniParc:UPI00001276EF", "UniRef:UniRef100_P78012", "UniRef:UniRef50_P47632", "UniRef:UniRef90_P78012" ], "nt": "ATGGCAAAGGAATTAGTATTTGGCAAGAATGCCCGCAATAAACTGCTAGCTGGTATTAATAAATTAGCAGACGCTGTTAAGGTAACCGTTGGTCCTAAAGGTCAAAACGTGATTTTAGGTCGGAAGTTTTCCAATCCTTTAATTACTAACGATGGAGTGACCATCGCTAAGGAAATTGAACTAACTGATCCTTTAGAAAACATTGGTGCTAAGGTTATTTCGGTAGCTGCTGTATCTACCAATGATATTGCTGGGGACGGTACTACCACTGCTACCATCTTGGCACAGGAAATGACTAACCGTGGTGTGGAAGCTGTTAATAATGGCGCTAATCCAGTTAATGTCCGCCGTGGGATTGAAGATGCTAGTCAGTTAATTATTACTGAGTTAGATAAGCGTTCCAAAAAGATTAATACCAACGAAGAAATCGAGCAAGTTGCAGCGATCTCTTCCGGTTCCAAGGAAATTGGGAAGCTGATTGCTCAAGCTATGGCCTTAGTTGGTAAAAACGGTGTCATTACCACCGATGATGCTAAGACGATTAACACTACCCTAGAAACCACTGAAGGGATTGAGTTTAAAGGTACTTACGCTTCCCCTTACATGGTTAGCGATCAAGAAAAAATGGAAGTGGTGTTAGACCAACCGAAGATCTTGGTCAGTGCGATGAAGATTAACACCATTAAGGAAATCTTACCGTTACTAGAAGGTAGCATGGAAAACGGTAACCCACTGTTAATCGTGGCACCTGACTTTGCTGAAGAAGTGGTAACTACTTTAGCGGTGAACAAGCTTCGCGGTACCATTAATGTAGTTGCTGTTAAATGTAACGAATACGGGGAACGCCAAAAGGCAGCTTTGGAAGATTTAGCCATTAGTACTGGCACCTTAGCTTACAACAATGAACTAGGTGGTGGCTTTAAGGATGTGACTGTAAACCACTTAGGGGAAGCTCGCCGTGTGCAAGTAGCCAAAGAAAAAACCACGGTTATTGGTGGTAAGGGTTCTAAGGAAACGATCCAAAAACACCTTGACCTTCTAAATGGTCGTTTAAAGCAAACTACCGAAAAGTACGATACCGATCTGTTAAAGGAACGGATTGCCCACCTCAGTCAAGGGGTAGCTGTAGTACGGGTTGGTGGTGCTACGGAACTAGCACAAAAAGAGTTAAAACTCCGCATTGAAGATGCCTTAAACTCGACTAAGGCAGCTGTTGAAGAAGGGATTATTTCCGGGGGTGGTATTGCTTTACTTAACGTATCCACTATCCTCAATGACAGTAAGTTGGCTGACAAGTACAAGGCAGAAACTAGTGCGGAAAACCTCAAGGAAATCCTGGTTGGTTATGAAATTGTGCGTAAGTCACTAGAAGCACCAGTGCGCCAAATTATTGAAAACTCCGGGGTTAACCCAGTTAAAGTATTTGCAGAACTACGCAGTGAAGCTGATGGTGTTGGTTTTGATGCTGAAACCAAAAAGAAGGTCGACATGATCCGCAGCGGTATTATTGACCCTACTAAGGTAACCAAAACTGCACTCGAAAAAGCTGCATCAGTAGCAAGTTCCTTAATTACCACCAGCGTTGCTGTGTATGATATTAAGGAAAACAAGGAAGGTAGCTTCCAAGAATAG", "aa": "MAKELVFGKNARNKLLAGINKLADAVKVTVGPKGQNVILGRKFSNPLITNDGVTIAKEIELTDPLENIGAKVISVAAVSTNDIAGDGTTTATILAQEMTNRGVEAVNNGANPVNVRRGIEDASQLIITELDKRSKKINTNEEIEQVAAISSGSKEIGKLIAQAMALVGKNGVITTDDAKTINTTLETTEGIEFKGTYASPYMVSDQEKMEVVLDQPKILVSAMKINTIKEILPLLEGSMENGNPLLIVAPDFAEEVVTTLAVNKLRGTINVVAVKCNEYGERQKAALEDLAISTGTLAYNNELGGGFKDVTVNHLGEARRVQVAKEKTTVIGGKGSKETIQKHLDLLNGRLKQTTEKYDTDLLKERIAHLSQGVAVVRVGGATELAQKELKLRIEDALNSTKAAVEEGIISGGGIALLNVSTILNDSKLADKYKAETSAENLKEILVGYEIVRKSLEAPVRQIIENSGVNPVKVFAELRSEADGVGFDAETKKKVDMIRSGIIDPTKVTKTALEKAASVASSLITTSVAVYDIKENKEGSFQE", "aa_hexdigest": "c64a2abdaf698cb2342265bde9053f1a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001276EF", "ncbi_nrp_id": "WP_010874930.1", "uniref100_id": "UniRef100_P78012", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001276EF", "RefSeq:WP_010874930.1", "UniRef:UniRef100_P78012" ] }, "ips": { "uniref100_id": "UniRef100_P78012", "uniref90_id": "UniRef90_P78012" }, "psc": { "uniref90_id": "UniRef90_P78012", "gene": "groL", "product": "chaperonin GroEL", "ec_ids": [ "5.6.1.7" ], "uniref50_id": "UniRef50_P47632", "cog_id": "COG0459", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_P47632", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47632" ], "product": "Chaperonin GroEL" }, "genes": [ "groL" ], "id": "GIHJHMOALN_1217", "locus": "GCA009939745_01217" }, { "type": "cds", "sequence": "contig_1", "start": 692041, "stop": 692391, "strand": "-", "frame": 1, "gene": "groES", "product": "co-chaperone GroES", "db_xrefs": [ "COG:COG0234", "COG:O", "GO:0005524", "GO:0005737", "GO:0044183", "KEGG:K04078", "RefSeq:WP_010874931.1", "SO:0001217", "UniParc:UPI0000127635", "UniRef:UniRef100_P75205", "UniRef:UniRef50_P47633", "UniRef:UniRef90_P75205" ], "nt": "ATGAAGATAACACCAATTCATGACAACATTTTGGTTTCACTAGTGGAATCAAACAAAGAAGAAGTGTCACAAAAAGGGATTATTACAGCACTAGCTAACCCTGATAAAAATGAAAGTGCCCACAAGGGAACTGTCGTTGCTTTAGGTGCAGGACCGGCATATGGTAAAAGCGAAAAACCGAAATATGCTTTTGGCATCGGTGACACGATCTACTTTAAGGAATACAGTGGTATTTCCTTTGAAGAGGAAGGTACCAAGTACAAGATTATTTCCTTGGAAGATGTCCTTGCTTTTGAAAAGCATGGTAATACAAAAACTACTACTGTAAAGAAAGGAGCTAAGAAAAAATAG", "aa": "MKITPIHDNILVSLVESNKEEVSQKGIITALANPDKNESAHKGTVVALGAGPAYGKSEKPKYAFGIGDTIYFKEYSGISFEEEGTKYKIISLEDVLAFEKHGNTKTTTVKKGAKKK", "aa_hexdigest": "ed0cbe8839984c80d949ab01ca30e622", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000127635", "ncbi_nrp_id": "WP_010874931.1", "uniref100_id": "UniRef100_P75205", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000127635", "RefSeq:WP_010874931.1", "UniRef:UniRef100_P75205" ] }, "ips": { "uniref100_id": "UniRef100_P75205", "uniref90_id": "UniRef90_P75205" }, "psc": { "uniref90_id": "UniRef90_P75205", "gene": "groES", "product": "co-chaperone GroES", "uniref50_id": "UniRef50_P47633", "cog_id": "COG0234", "cog_category": "O", "kegg_orthology_id": "K04078", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0044183" ] }, "pscc": { "uniref50_id": "UniRef50_P47633", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47633" ], "product": "Co-chaperonin GroES" }, "genes": [ "groES" ], "id": "GIHJHMOALN_1218", "locus": "GCA009939745_01218" }, { "type": "cds", "sequence": "contig_1", "start": 692579, "stop": 692965, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_575", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014325607.1", "SO:0001217", "UniParc:UPI0001DD6434", "UniRef:UniRef100_A0AAX0SS40", "UniRef:UniRef50_P75204", "UniRef:UniRef90_P75204" ], "nt": "ATGCTGCAAAATTTAGCTTTAAGCTTTCCTTTTATTACGCGTTTCTTCCAAAAGCAAATGCTTGGTTCCCAGAATTCTAGTGGTAAAACTCCTGGTTTCAATGAAGCCGAAGGAATAACGAGTAACATTTTCCAAATTGCTGGTGGAATTAGCCTTTTAGTTATTTTGTTGTTAATCATTGGTTTCTTGAGTTGTTTGTTGGGTGGCATCTTCCTACATAAGCACAAACACGCTGAAGTGGGTAGTCCCGCTCATGCAAAAACGAAAAATCTATTTGTTGCTTTCTTTGTTGTCGGTAGCTTATTGTTGTTAGTGGCCGTAGTCATGTTAATTGCTTTCGGTGTGCTTGATGCTAGTCTGCCACTTCCAAAAGAAAATAACTCTTAA", "aa": "MLQNLALSFPFITRFFQKQMLGSQNSSGKTPGFNEAEGITSNIFQIAGGISLLVILLLIIGFLSCLLGGIFLHKHKHAEVGSPAHAKTKNLFVAFFVVGSLLLLVAVVMLIAFGVLDASLPLPKENNS", "aa_hexdigest": "d40087e101e7fb6e59f9bb4b6e8b0ef0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6434", "ncbi_nrp_id": "WP_014325607.1", "uniref100_id": "UniRef100_A0AAX0SS40", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6434", "RefSeq:WP_014325607.1", "UniRef:UniRef100_A0AAX0SS40" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SS40", "uniref90_id": "UniRef90_P75204" }, "psc": { "uniref90_id": "UniRef90_P75204", "product": "Uncharacterized protein MPN_575", "uniref50_id": "UniRef50_P75204", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75204", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75204" ], "product": "Uncharacterized protein MPN_575" }, "genes": [], "id": "GIHJHMOALN_1219", "locus": "GCA009939745_01219" }, { "type": "cds", "sequence": "contig_1", "start": 693400, "stop": 693657, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "TTGCAACAAGCCAACATTGTGTTGAACATGAACACGGTTCCTTTCGATAAGAATCCTGCCATAAATCCTTCCGGTATTCGTATTGGTACACCTGCCATGACTACTCGTGGCTTTAAGGAAAAGCACTTCCTTTATGTTGCTGCCTTAATTGACAAGATTATTAAATCTGACGGTAATAAGAAAGTTATTAAAGAAGTAAAAAAAGCGGTTTTAAAACTGTTAGAACGTTTTCCGTTGTATAAGGGATTGGAGTATTAG", "aa": "MQQANIVLNMNTVPFDKNPAINPSGIRIGTPAMTTRGFKEKHFLYVAALIDKIIKSDGNKKVIKEVKKAVLKLLERFPLYKGLEY", "aa_hexdigest": "1c7ac642cdc9098ecaa540745a491ff2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 49, "aa_cov": 0.5764705882352941, "hmm_cov": 0.9795918367346939, "evalue": 1.5988883875459666e-14, "score": 48.78683853149414, "start": 2, "stop": 50 } ], "seq_stats": { "molecular_weight": 9617.351999999995, "isoelectric_point": 9.939171409606931 }, "id": "GIHJHMOALN_1220", "locus": "GCA009939745_01220" }, { "type": "cds", "sequence": "contig_1", "start": 693658, "stop": 694374, "strand": "-", "frame": 1, "gene": "glyA", "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "COG:COG0112", "COG:E", "EC:2.1.2.1", "GO:0004372", "GO:0005829", "GO:0019264", "GO:0030170", "GO:0035999", "KEGG:K00600", "SO:0001217", "UniRef:UniRef50_P47634", "UniRef:UniRef90_P78011" ], "nt": "GTGCAACCGCACTCCGGTTCTTCTGCTAACTATGCCGTGTACTTAGCTCTACTTAAACCTGGCGATGCCATTTTAGGGCTTGACCTTAACTGTGGTGGGCACTTAACTCACGGTAATAAATTTAACTTTTCGGGTAAACAGTATCAGCCTTATTCCTACACCATCAACCCCGAAACCGAGATGTTGGACTATGATGAGGTGCTACGTGTTGCTCGGGAGGTAAAACCTAAACTAATTATCTGCGGTTTTTCCAATTACTCGAGAACCGTTGACTTTGAGCGCTTTAGTGCAATTGCGAAAGAAGTTGGTGCTTACCTTTTAGCGGACATTGCCCACATTGCGGGATTGGTAGCAGCTGGCTTGCACCCCAATCCCTTACCTTACGCTGATGTGGTTACATCTACTACCCACAAAACACTGCGCGGTCCACGGGGAGGGTTAATTATGTCCAATAACGAAGCGATTATTCGCAAATTGGACAGCGGTGTCTTTCCGGGTTGTCAAGGTGGTCCCTTACAGCACGTAATTGCGGCTAAGTATGTTTGTTTTAAAGAAGCTTTACAACCAAAATACAAGCAGTACATCCAAAACGTTAAGACCAATGCAGCGAGCATGGCTAGTTGGTTCAAACAACAAGGTTACCGCGTCATTTCTAACGGAACTGATACCCACTTGTTCTCGCTTGATGTCGGTAAGGGTAAAGATGTTTCACAATGA", "aa": "MQPHSGSSANYAVYLALLKPGDAILGLDLNCGGHLTHGNKFNFSGKQYQPYSYTINPETEMLDYDEVLRVAREVKPKLIICGFSNYSRTVDFERFSAIAKEVGAYLLADIAHIAGLVAAGLHPNPLPYADVVTSTTHKTLRGPRGGLIMSNNEAIIRKLDSGVFPGCQGGPLQHVIAAKYVCFKEALQPKYKQYIQNVKTNAASMASWFKQQGYRVISNGTDTHLFSLDVGKGKDVSQ", "aa_hexdigest": "8cc5e5c0d67e5f8f13ea84f29180dfa4", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FEF2304", "query_cov": 0.9705882352941176, "subject_cov": 0.9352226720647774, "identity": 0.628, "score": 286.0, "evalue": 6.81e-95, "valid": false, "product": "serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A661GFR2", "kegg_orthology_id": "K00600" }, "genes": [ "glyA" ], "truncated": "both", "pseudogene": { "start": 693400, "stop": 694620, "observations": { "mutation": [ 693660, 694383 ], "stop": [ 693660, 694383 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78011", "query_cov": 1.0, "subject_cov": 0.5862068965517241, "identity": 0.992, "score": 484.0, "evalue": 2.25e-170, "gene_start": 1, "gene_end": 238, "reference_start": 83, "reference_end": 320, "reference_sequence": "MEPKIRRILNKELQRQRDCICLIASENYVSRDILEVTGSILTNKYAEGYPTRRFYEGCEVVDESESLAINTCKELFGAKWANVQPHSGSSANYAVYLALLKPGDAILGLDLNCGGHLTHGNKFNFSGKQYQPYSYTINPETEMLDYDEVLRVAREVKPKLIICGFSNYSRTVDFERFSAIAKEVGAYLLADIAHIAGLVAAGLHPNPLPYTDVVTSTTHKTLRGPRGGLIMSNNEAIIRKLDSGVFPGCQGGPLQHVIAAKYVCFKEALQPKYKQYIQNVKTNAASMASWFKQQGYRVISNGTDTHLFSLDVGKGKDVSQWLQQANIVLNMNTVPFDKNPAINPSGIRIGTPAMTTRGFKEKHFLYVAALIDKIIKSDGNKKVIKEVKKAVLKLLERFPLYKGLEY" }, "paralog": false, "identity": 0.9926108374384236, "score": 809.0, "evalue": 5.16e-301, "description": "Internal stop codon at 693660, 694383. Nonsense mutation around 693660, 694383.", "psc": { "uniref90_id": "UniRef90_P78011", "gene": "glyA", "product": "Serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_P47634", "cog_id": "COG0112", "cog_category": "E", "kegg_orthology_id": "K00600", "go_ids": [ "GO:0005829", "GO:0004372", "GO:0030170", "GO:0019264", "GO:0035999" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78011", "EC:2.1.2.1", "UniRef:UniRef50_P47634", "COG:COG0112", "COG:E", "KEGG:K00600", "GO:0005829", "GO:0004372", "GO:0030170", "GO:0019264", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_P47634", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47634" ], "product": "Serine hydroxymethyltransferase" } }, "id": "GIHJHMOALN_1221", "locus": "GCA009939745_01221" }, { "type": "cds", "sequence": "contig_1", "start": 694381, "stop": 694620, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "ATGGAACCAAAGATTAGAAGAATCCTCAATAAGGAATTGCAAAGACAAAGAGATTGCATTTGTTTAATAGCATCAGAGAACTATGTGAGTAGGGACATCCTAGAGGTCACTGGTTCCATTTTGACCAATAAGTACGCTGAGGGTTATCCTACTAGACGCTTTTATGAAGGTTGTGAGGTAGTTGATGAATCAGAGAGCTTAGCCATTAATACCTGTAAGGAATTATTTGGTGCGAAGTGA", "aa": "MEPKIRRILNKELQRQRDCICLIASENYVSRDILEVTGSILTNKYAEGYPTRRFYEGCEVVDESESLAINTCKELFGAK", "aa_hexdigest": "99b359e63f0562419aa7e22af2aef923", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1F2Y6L7", "query_cov": 0.9367088607594937, "subject_cov": 0.8505747126436781, "identity": 0.5539999999999999, "score": 83.6, "evalue": 1.76e-19, "valid": false, "product": "Serine hydroxymethyltransferase-like domain-containing protein", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A838MK70", "kegg_orthology_id": "K00600" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 77, "aa_cov": 0.9746835443037974, "hmm_cov": 1.0, "evalue": 1.8062091092474533e-29, "score": 98.00299072265625, "start": 2, "stop": 78 } ], "seq_stats": { "molecular_weight": 9119.335399999994, "isoelectric_point": 5.453383064270019 }, "id": "GIHJHMOALN_1222", "locus": "GCA009939745_01222" }, { "type": "cds", "sequence": "contig_1", "start": 694698, "stop": 694835, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI001004F7CA", "UniRef:UniRef100_A0AB38W5S7", "UniRef:UniRef50_A0AB38W5S7", "UniRef:UniRef90_A0AB38W5S7" ], "nt": "ATGAGTGTAGTGTTGTTTGGCAAATTGGGTATAGTAATGGGTAGTATTGCGGTTGCCAAAGATAAGATCATAACCAATGCTGCTCTAGTTCGGATTGAGGGAATTGCCCTCACCCTGAAAGCGCAGCATTTGGACTAA", "aa": "MSVVLFGKLGIVMGSIAVAKDKIITNAALVRIEGIALTLKAQHLD", "aa_hexdigest": "a682c9f0a1623f3cff503bf06613f5af", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001004F7CA", "uniref100_id": "UniRef100_A0AB38W5S7", "db_xrefs": [ "SO:0001217", "UniParc:UPI001004F7CA", "UniRef:UniRef100_A0AB38W5S7" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W5S7", "uniref90_id": "UniRef90_A0AB38W5S7" }, "psc": { "uniref90_id": "UniRef90_A0AB38W5S7", "uniref50_id": "UniRef50_A0AB38W5S7" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5S7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4719.697099999999, "isoelectric_point": 9.52289905548096 }, "id": "GIHJHMOALN_1223", "locus": "GCA009939745_01223" }, { "type": "cds", "sequence": "contig_1", "start": 694782, "stop": 694985, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGTATGGTCATGGTTTAGGAATCGATCAGGTTAACCTAAGAAAAGGGACTTCTAGTTCTTTAGTGATTAACCAAAAGCGTCAAATTGTGGGAATCTATTTTGCTACGGTAATTACTAATCCCAAGAAGGCTGTTCGTAACGATGTTGGTTTAGTCCAAATGCTGCGCTTTCAGGGTGAGGGCAATTCCCTCAATCCGAACTAG", "aa": "MYGHGLGIDQVNLRKGTSSSLVINQKRQIVGIYFATVITNPKKAVRNDVGLVQMLRFQGEGNSLNPN", "aa_hexdigest": "8973777533218428a702c0160bd05934", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 34, "aa_cov": 0.5074626865671642, "hmm_cov": 1.0, "evalue": 3.34120366949623e-08, "score": 28.994007110595703, "start": 2, "stop": 35 } ], "seq_stats": { "molecular_weight": 7357.3937000000005, "isoelectric_point": 10.277243614196774 }, "id": "GIHJHMOALN_1224", "locus": "GCA009939745_01224" }, { "type": "cds", "sequence": "contig_1", "start": 695046, "stop": 695687, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_577", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75203", "UniRef:UniRef90_P75203" ], "nt": "TTGAGCAATCCGAAGGACTATGCACCGTTTAATAAAGCTAGCATTGGCAACAGTTTAACTACGGTATTTTGTCTAGGGAAATATATTAACCCTCAATTGTTTAAGCTACGTACTGATGTAAGCAACGCCTTTGTGTCAATACAAACTTCCACTATTCCCAAAACAGCCTTTGTAGCACGCGACTTTGTGCCCTTACAAAACCGCGGTAACCAGTGGGTAGCACCAGTTCGTGCATCAGAAGATGATCCTGCACTAGCAAAAAGCTATTTGGATTTTGCGATCATAGAAGTTCCACTCTTTTTACACAACCAAATGGATAAACAGATCTATGACCACTTTATGCGCCCCGCCATCAATACCTATGAACGGTTAGGTAACAGTGTGGGAATCTTTGCTTACCAACCTATGGCAAGCTTTAAGCGAGATAGCTATTTTGCTTTAGGTTATCCCCAAGTAGAGAGCAATATTGCTGCACTTAACTTGAACCAAACTGAAGTTAAACCTACTCGCCCAGAGGATGTTGCTCAAGTTACTTTTAAAGAGCCTTGGTCAGTAGATCACCACCGCGAAATTCCTACTTTGACTACCAATCAACTTACCACCATTAAAACAAAACACTTTTCTGGTAGTAAGTTATCTTGA", "aa": "MSNPKDYAPFNKASIGNSLTTVFCLGKYINPQLFKLRTDVSNAFVSIQTSTIPKTAFVARDFVPLQNRGNQWVAPVRASEDDPALAKSYLDFAIIEVPLFLHNQMDKQIYDHFMRPAINTYERLGNSVGIFAYQPMASFKRDSYFALGYPQVESNIAALNLNQTEVKPTRPEDVAQVTFKEPWSVDHHREIPTLTTNQLTTIKTKHFSGSKLS", "aa_hexdigest": "0ad465293935d5aa7e54199c4b8c9619", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 694782, "stop": 695942, "observations": { "mutation": [ 695048, 695768, 695780, 695801, 695900 ], "stop": [ 695048, 695768, 695780, 695801, 695900 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75203", "query_cov": 1.0, "subject_cov": 0.5518134715025906, "identity": 0.995, "score": 429.0, "evalue": 2.31e-149, "gene_start": 1, "gene_end": 213, "reference_start": 86, "reference_end": 298, "reference_sequence": "MKTVTAAGGVKRKEWKTNYLINTIPKKSEALHDLYDLSFSMQLANNKWRVFGTGWLIDWKKPKRTHNLSEPFYLYLATNLHIAVALSNPKDYAPFNKASIGNSLTTVFCLGKYINPQLFKLRTDVSNAFVSIQTSTIPKTAFVARDFVPLQNRGNQWVAPVRASEDDPALAKSYLDFAIIEVPLFLHNQMDKQIYDHFMRPAINTYERLGNSVGIFAYQPMASFKRDSYFALGYPQVESNIAALNLNQTEVKPTRPEDVAQVTFKEPWSVDHHREIPTLTTNQLTTIKTKHFSGSKLSWPFDHTKSFKIKNKWLGQNYQMYGHGLGIDQVNLRKGTSSSLVINQKRQIVGIYFATVITNPKKAVRNDVGLVQMLRFQGEGNSLNPN" }, "paralog": false, "identity": 0.9844559585492227, "score": 759.0, "evalue": 2.86e-282, "description": "Internal stop codon at 695048, 695768, 695780, 695801, 695900. Nonsense mutation around 695048, 695768, 695780, 695801, 695900.", "psc": { "uniref90_id": "UniRef90_P75203", "product": "Uncharacterized protein MPN_577", "uniref50_id": "UniRef50_P75203", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75203", "UniRef:UniRef50_P75203" ] }, "pscc": { "uniref50_id": "UniRef50_P75203", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75203" ], "product": "Uncharacterized protein MPN_577" } }, "id": "GIHJHMOALN_1225", "locus": "GCA009939745_01225" }, { "type": "cds", "sequence": "contig_1", "start": 697049, "stop": 697471, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_580", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "RefSeq:WP_014325610.1", "SO:0001217", "UniParc:UPI0002386D12", "UniRef:UniRef100_A0AAV5N765", "UniRef:UniRef50_P75200", "UniRef:UniRef90_P75200" ], "nt": "ATGACCGCCCAGTATTACCAACTAGACGAAAAGTTTAATCAGCAAACCTATGAACAGTATGGTAAAGGCTTAGCCTTAAATGATTTGTATATCCGCAGGGGCGCTTCTGGTAGTTTAGTCTTCAATCAGGATCAACAAATATCAGGCATCTTTTTTGCAGTAGCTAGTCATTCCGACCCTAAACCTGGTGAAAAAACTCTGGTACAACTGTTACGCTTGCCAGTTGATGAAAGTACTACAGCGACGGTAAAGGACAACTGTGTGCCCTATGATTTAATCTTTGGCAACCGCAATACTACCCATTACTATACCCAATTTGCCAAGCAACACCACACCCATTTGTATGAGCAAATTCAACAAAGTAATGACCAGTTAATTAAGTTTATTGATCGCGAAAATGTGTCCTGTTCAATGGTTTCGTAA", "aa": "MTAQYYQLDEKFNQQTYEQYGKGLALNDLYIRRGASGSLVFNQDQQISGIFFAVASHSDPKPGEKTLVQLLRLPVDESTTATVKDNCVPYDLIFGNRNTTHYYTQFAKQHHTHLYEQIQQSNDQLIKFIDRENVSCSMVS", "aa_hexdigest": "ac553a85c54f196a69ccad62e60b5e83", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386D12", "ncbi_nrp_id": "WP_014325610.1", "uniref100_id": "UniRef100_A0AAV5N765", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386D12", "RefSeq:WP_014325610.1", "UniRef:UniRef100_A0AAV5N765" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N765", "uniref90_id": "UniRef90_P75200" }, "psc": { "uniref90_id": "UniRef90_P75200", "product": "Uncharacterized protein MPN_580", "uniref50_id": "UniRef50_P75200", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_P75200", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75200" ], "product": "Uncharacterized protein MPN_580" }, "genes": [], "id": "GIHJHMOALN_1226", "locus": "GCA009939745_01226" }, { "type": "cds", "sequence": "contig_1", "start": 697554, "stop": 697940, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_581", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75199", "UniRef:UniRef90_P75199" ], "nt": "ATGGCTTATGCAGCGGTGGACTTTGTTGATTATCGGTTTGACAATAAACATTGGAAATCCTTAAATGAAATTTGTCCGGAAGTTGGTTTAAAACCTTATGCTGATTTTGCCGTGTTAGAAGTACCCTTGTACTTAAACAACAAGTTGGATTACCAAGTATGGGAAAACTTTATCAAACCCGCCATTGCTACCTATAAGGCCTTAGGTGATAGTACAAACTTGTTTGCAAGTACGTCATCTTCTGATTTACAAAATGACACCTATTTTGCTCTCGGTTATCCCTTACTAGAGAGTAACATTGATGCGATTCACTTTAACCAAACAGGTGCCACTTTAACTAGTGTTCCGGTTAAAGACCGCATTAAAGTTAAAAACCAAAGCTTCTGA", "aa": "MAYAAVDFVDYRFDNKHWKSLNEICPEVGLKPYADFAVLEVPLYLNNKLDYQVWENFIKPAIATYKALGDSTNLFASTSSSDLQNDTYFALGYPLLESNIDAIHFNQTGATLTSVPVKDRIKVKNQSF", "aa_hexdigest": "7af1985afa574e988a4eafa9b8f1ad0b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 697539, "stop": 698240, "observations": { "mutation": [ 697556, 698174, 698207 ], "stop": [ 697556, 698174, 698207 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75199", "query_cov": 1.0, "subject_cov": 0.4830188679245283, "identity": 1.0, "score": 264.0, "evalue": 1.47e-87, "gene_start": 1, "gene_end": 128, "reference_start": 133, "reference_end": 260, "reference_sequence": "MQLEKVPRAEYQTKWATNTVPKPTDFYHQLYNLSFSLTLTSQRWNTYGTGWLIDWKDTSTNENKFTAYLATNFHVADTLKNPHDYPPYNQVDNTQDLTTSFRIGKYTDPSLFGLYTNVKHAFVDVQLAALPKMAYAAVDFVDYRFDNKHWKSLNEICPEVGLKPYADFAVLEVPLYLNNKLDYQVWENFIKPAIATYKALGDSTNLFASTSSSDLQNDTYFALGYPLLESNIDAIHFNQTGATLTSVPVKDRIKVKNQSFWTLNK" }, "paralog": false, "identity": 0.9871244635193133, "score": 468.0, "evalue": 2.35e-170, "description": "Internal stop codon at 697556, 698174, 698207. Nonsense mutation around 697556, 698174, 698207.", "psc": { "uniref90_id": "UniRef90_P75199", "product": "Uncharacterized protein MPN_581", "uniref50_id": "UniRef50_P75199", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75199", "UniRef:UniRef50_P75199" ] }, "pscc": { "uniref50_id": "UniRef50_P75199", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75199" ], "product": "Uncharacterized protein MPN_581" } }, "id": "GIHJHMOALN_1227", "locus": "GCA009939745_01227" }, { "type": "cds", "sequence": "contig_1", "start": 698427, "stop": 698534, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCAGTTTGCAAAAAAATAACACTCACTTTTATGGGCAAATTAAATCTGCGCGAGACCCTAGTTTAAAATATGTAGATAACGAATCAAAGGTAAAAAATACTTAA", "aa": "MPSLQKNNTHFYGQIKSARDPSLKYVDNESKVKNT", "aa_hexdigest": "0639fd0271805c332aaa09bb3493acd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4039.487000000001, "isoelectric_point": 9.601679420471193 }, "id": "GIHJHMOALN_1228", "locus": "GCA009939745_01228" }, { "type": "cds", "sequence": "contig_1", "start": 698617, "stop": 698772, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGTATGGCAAGGGAATTGGGATCACTAATGGTAGTTTAAGTCGTGGCGCTTCGGGTTCCTTAGTCTTAAATAACAAGCGACAGATTGTGGCCATTTACTTTGCTAGTAGAATCACTGAAACACAAGAATGGGGTCTAGCGCAGCTGTTACGCTGA", "aa": "MYGKGIGITNGSLSRGASGSLVLNNKRQIVAIYFASRITETQEWGLAQLLR", "aa_hexdigest": "2591b3d16ac1c27cebacf4a224baedf9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 34, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 1.5824917996884134e-10, "score": 36.63825225830078, "start": 2, "stop": 35 } ], "seq_stats": { "molecular_weight": 5540.316699999999, "isoelectric_point": 10.419847679138183 }, "id": "GIHJHMOALN_1229", "locus": "GCA009939745_01229" }, { "type": "cds", "sequence": "contig_1", "start": 699235, "stop": 699789, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MPN_582", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75198", "UniRef:UniRef90_P75198" ], "nt": "TTGGCACTAACTGTTTTAAGTGCTTGTTCAGCCTTTCGAAAACCTGATAAATTCGAAGGGTATTACATTAACAATATTCCTGGTAGTGCAGAAATCGATCATGCCAACTATGATTTAACCTTTTCTTTAGGTTTTTTTAACAGAATTGAAAAAACCGAGAAAGATAAAAGTCAAATAGATTTTGGTTCCATTCACGGAACGGGTTGGTTAATTGACTGGAAGGAAGTTGACAAGAAAGATAAAAAAGCTAATAAATTTACTGTTTACTTAGCTACTAATTTACACGTCATACAGGCCTTAAAAAACCGAGAAGATCATCCGCCTTACAACCAATTTGATATCAATTTTGTGCGAACCGTTGATTTTAGAATTGGCAAATACACTGATGTTAAACAATTTGTCGCACCAAGCAAATTAGGCTTACCCAACTCTACCCAAGCTTTTGTAGCAGCACAACCAACGGTACTTCCTAAAACTGCTTTCATTGCACATGACTTTGTGAACTACACTTTATCCAAAGATCAAAAGAATAAAAAACAAAGAGAACAGCAATGA", "aa": "MALTVLSACSAFRKPDKFEGYYINNIPGSAEIDHANYDLTFSLGFFNRIEKTEKDKSQIDFGSIHGTGWLIDWKEVDKKDKKANKFTVYLATNLHVIQALKNREDHPPYNQFDINFVRTVDFRIGKYTDVKQFVAPSKLGLPNSTQAFVAAQPTVLPKTAFIAHDFVNYTLSKDQKNKKQREQQ", "aa_hexdigest": "34495acd0dab58c2013c42c94769968b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 698932, "stop": 699831, "observations": { "mutation": [ 699237, 699828 ], "stop": [ 699237, 699828 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75198", "query_cov": 1.0, "subject_cov": 0.4043956043956044, "identity": 0.995, "score": 377.0, "evalue": 2.27e-128, "gene_start": 1, "gene_end": 184, "reference_start": 15, "reference_end": 198, "reference_sequence": "MWVALKRFCFLSGLLALTVLSACSAFRKPDKFEGYYINNIPGSAEIDHANYDLTFSLGFFNRIEKTEKDKSQIDFGSIHGTGWLIDWKEVDKKDKKANKFTVYLATNLHVIQALKNREDHPPYNQFDINFVRTVDFRIGKYTDVKQFVAPSKLGLPNSTQAFVAAQPTVLPKTAFIAHDFVNYTLSKDQKNKKQREQQWKVQIKPNKDEVHPYADFAVLELPLFLNNSSDRQIFDHFIQPAIRAYKQLGDSLNIFAYPTLDQFKHSHYYVLGYPYIAKKLPTLFVNQTGKEKTVPGETAQIPNDQPFVSTINEEGPHLGRIKSDKFNGGTWAWNHDNTKNFPFNRQFRGKEYQMYGKGIGITNGSLSRGASGSLVLNNKRQIVAIYFASRITETQEWGLAQLLRWKPRSVLNEEKDSVAYDLIFGNSNTKKYYAQFAKKITLTFMGKLNLRETLV" }, "paralog": false, "identity": 0.9933110367892977, "score": 605.0, "evalue": 1.35e-220, "description": "Internal stop codon at 699237, 699828. Nonsense mutation around 699237, 699828.", "psc": { "uniref90_id": "UniRef90_P75198", "product": "Uncharacterized lipoprotein MPN_582", "uniref50_id": "UniRef50_P75198", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75198", "UniRef:UniRef50_P75198", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75198", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75198" ], "product": "Uncharacterized lipoprotein MPN_582" } }, "id": "GIHJHMOALN_1230", "locus": "GCA009939745_01230" }, { "type": "cds", "sequence": "contig_1", "start": 699906, "stop": 700049, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCTATGACTTAATCTTTGGTAACAAAGACACTAAAAAGTACTACGCTCAATTTGCTAAACAACATAAAACCCATTTGTTTGAGCAAATTAAGCAAAGCAACGACCAACAATTTACCTTTGTTAAAAACCAAAAGTCTTAA", "aa": "MAYDLIFGNKDTKKYYAQFAKQHKTHLFEQIKQSNDQQFTFVKNQKS", "aa_hexdigest": "093ceb9e08a6c6cb4bb8c2259fa78da6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5687.358000000002, "isoelectric_point": 9.574667167663574 }, "id": "GIHJHMOALN_1231", "locus": "GCA009939745_01231" }, { "type": "cds", "sequence": "contig_1", "start": 700302, "stop": 700664, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_583", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197", "UniRef:UniRef90_P75197" ], "nt": "GTGTTAGAGCTAACCTTATTTTTAGATAACCCAGTTGATAAAAAGGTGTTTGACCATTTTATCCAACCAGCCATCAAAACTTATGAGCAATTAGGTAATAGCTTAAAGCTGTTTGCCAAGCCAACATTAACTGAACTCAAAAACCACCGCTATTATTTATCGGGTTATCCCTTCTTGGAAAACAAGGTTAATGTATTGAGTGTTAATCAAATTAAGAAAGATACATCTACAGAAACAACAATAAATGGACAAACAGTACAACAAAGTAACCACGAACAACCCCTTATTGTGCGATCGACAATAGCAAAGCCAACCCTAATTAGAAATCAAGGTGATGACTTTAATGGCAGTAACTGGGTTTGA", "aa": "MLELTLFLDNPVDKKVFDHFIQPAIKTYEQLGNSLKLFAKPTLTELKNHRYYLSGYPFLENKVNVLSVNQIKKDTSTETTINGQTVQQSNHEQPLIVRSTIAKPTLIRNQGDDFNGSNWV", "aa_hexdigest": "790d2211dd4e7a156dd27d13919386a5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 700269, "stop": 700946, "observations": { "mutation": [ 700304, 700748 ], "stop": [ 700304, 700748 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75197", "query_cov": 1.0, "subject_cov": 0.5333333333333333, "identity": 0.992, "score": 241.0, "evalue": 3.8e-79, "gene_start": 1, "gene_end": 120, "reference_start": 95, "reference_end": 214, "reference_sequence": "MPNNAQAFVNVQTTVIPKTAFTAHDFVNYTLPKDKKKQNDTENKKKQPKDGENDKQKEQAETQPFEWIQQKDADDKKESNTANTSDSLAYADFAVLELTLFLDNPVDKKVFDHFIQPAIKTYEQLGNSLKLFAKPTLTELKNHRYYLSGYPFLENKVNVLSVNQIKKDTSTETTINGQTVQQSNHEQPLIVRSTIAKPTLIRNQGDDFNGSNWVWNYDNSRVFRF" }, "paralog": false, "identity": 0.9866666666666667, "score": 448.0, "evalue": 2.35e-163, "description": "Internal stop codon at 700304, 700748. Nonsense mutation around 700304, 700748.", "psc": { "uniref90_id": "UniRef90_P75197", "product": "Uncharacterized protein MPN_583", "uniref50_id": "UniRef50_P75197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75197", "UniRef:UniRef50_P75197" ] }, "pscc": { "uniref50_id": "UniRef50_P75197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197" ], "product": "Uncharacterized protein MPN_583" } }, "id": "GIHJHMOALN_1232", "locus": "GCA009939745_01232" }, { "type": "cds", "sequence": "contig_1", "start": 700746, "stop": 700946, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCAATAATGCTCAAGCCTTTGTTAATGTCCAAACCACAGTAATTCCCAAAACCGCTTTTACAGCGCATGACTTTGTCAACTACACTTTGCCAAAAGACAAAAAGAAGCAAAATGATACAGAAAATAAAAAGAAGCAACCAAAGGATGGAGAAAACGATAAGCAAAAGGAGCAAGCGGAAACCCAACCGTTTGAGTGA", "aa": "MPNNAQAFVNVQTTVIPKTAFTAHDFVNYTLPKDKKKQNDTENKKKQPKDGENDKQKEQAETQPFE", "aa_hexdigest": "27f00c9c5972bf2d144a27d58588c4bf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7593.327200000004, "isoelectric_point": 7.967276954650878 }, "id": "GIHJHMOALN_1233", "locus": "GCA009939745_01233" }, { "type": "cds", "sequence": "contig_1", "start": 701142, "stop": 701387, "strand": "-", "frame": 2, "gene": null, "product": "DUF31 family putative serine protease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00143DB747", "UniRef:UniRef90_UPI00143DB747" ], "nt": "ATGATTGTGGCTTTCAAGCGCCTTGGTTTTCTCGCTAGCATAGCTTCCTTAACTGTATTAAGTGCTTGTGCTGCAGTGCCACTAGAAAAGTGGGATCAGTTCTATGTAAGTAATATTCCTAGTAGTGGTTCGATTCACCGTGCCAACTATGATTTAACCTTTGCTTTAACTTTTACTAATAAAACTACTAACAATGGGCAAGATAACTTTAGCGCCTTTTCTGGCACTGGCTGGCTCATTGATTGA", "aa": "MIVAFKRLGFLASIASLTVLSACAAVPLEKWDQFYVSNIPSSGSIHRANYDLTFALTFTNKTTNNGQDNFSAFSGTGWLID", "aa_hexdigest": "27a4ae5b6919c157144c26f8a16e0504", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00143DB747", "query_cov": 0.9629629629629629, "subject_cov": 0.896551724137931, "identity": 1.0, "score": 158.0, "evalue": 5.08e-49, "valid": true, "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_UPI00143DB747" }, "pscc": { "uniref50_id": "UniRef50_UPI00143DB747", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00143DB747" ], "product": "DUF31 family putative serine protease" }, "genes": [], "id": "GIHJHMOALN_1234", "locus": "GCA009939745_01234" }, { "type": "cds", "sequence": "contig_1", "start": 701451, "stop": 701606, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTATGATTTAATCTTTGGTAATAAAAATACCACAAAATACTATGCTCAGTTTGCCAAAGAACACAAAACCCATTTATACGAGCAAATTAAGCAAATTAACAATGATGAATTCCGCTTTGTCGAAAAGACTAAAAGGCATAACTTGGGGTAA", "aa": "MAYDLIFGNKNTTKYYAQFAKEHKTHLYEQIKQINNDEFRFVEKTKRHNLG", "aa_hexdigest": "282fc9ff7f7dc1613d642b8764a0caa5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6222.954599999999, "isoelectric_point": 9.347158241271973 }, "id": "GIHJHMOALN_1235", "locus": "GCA009939745_01235" }, { "type": "cds", "sequence": "contig_1", "start": 701643, "stop": 701858, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_584", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "SO:0001217", "UniRef:UniRef50_P75196", "UniRef:UniRef90_P75196" ], "nt": "TTGAACTATGATCTCTCCCAGAAATTTAAGGTTTATCTTGAGTTTTCGGGCAAAAAGTACCGAATCTATGGCCAAAGTATAGGCATTAGTCATCTTGATTTAGGCGGTGGATCTTCTGGTTCTTTGTTGTTAAATGATAAGAAACAAATCGCTGGCATTTACTTTGGCGTTGATGGTGCTAACAATGAATTAGGCTTAGCACAGTTATTACGCTGA", "aa": "MNYDLSQKFKVYLEFSGKKYRIYGQSIGISHLDLGGGSSGSLLLNDKKQIAGIYFGVDGANNELGLAQLLR", "aa_hexdigest": "1aa1fda7670c3abf5a06f733d472adf5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 701451, "stop": 701858, "observations": { "mutation": [ 701645 ], "stop": [ 701645 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75196", "query_cov": 1.0, "subject_cov": 0.5259259259259259, "identity": 1.0, "score": 141.0, "evalue": 5.23e-42, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MNYDLSQKFKVYLEFSGKKYRIYGQSIGISHLDLGGGSSGSLLLNDKKQIAGIYFGVDGANNELGLAQLLRWKPETYHNDEARSVAYDLIFGNKNTTKYYAQFVKEHKTHLYEQIKQINNDEFRFVEKTKRHNLG" }, "paralog": false, "identity": 0.9777777777777777, "score": 271.0, "evalue": 2.26e-95, "description": "Internal stop codon at 701645. Nonsense mutation around 701645.", "psc": { "uniref90_id": "UniRef90_P75196", "product": "Uncharacterized protein MPN_584", "uniref50_id": "UniRef50_P75196", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75196", "UniRef:UniRef50_P75196", "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_P75196", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75196" ], "product": "Uncharacterized protein MPN_584" } }, "id": "GIHJHMOALN_1236", "locus": "GCA009939745_01236" }, { "type": "cds", "sequence": "contig_1", "start": 701944, "stop": 702393, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75195", "UniRef:UniRef90_A0AB38W8P6" ], "nt": "TTGCCCAATAATGCTCAAGCCTTTGTTAATGTCCAAACCACAGTAATTCCCAAAACCGCTTTTGCTGCTAGGGACTTTGTGGATTACAGTTTTCCTCAAGAACAAAAAGATAAAGAAAAGAGAAAACAACAATGGGTGAAAAACAGTCATACTAAGAATTCTGATGTCCAGCCTTTTGCTGAGTTTGCAATTTTAGAAATTCCCTTATTTTCGAAAAGTAAAGTAGATCGCAAAATCTTTAATCACTTTATTCAACCAGCCATACGCACTTATAAACAGTTAGGTGATAGCCTTAACATTTTTGCTAACCCCACACTGGATCAATTAAAACAAAATCGCTATTATGTGTTAGGTTATCCTTTTTTAAAAAATAAAGTGTCATCACTATTTCTTAACCAAACGGGAAAAAAGAAAGGAATATTTAGGCGAAAATACACAAATATCCCATGA", "aa": "MPNNAQAFVNVQTTVIPKTAFAARDFVDYSFPQEQKDKEKRKQQWVKNSHTKNSDVQPFAEFAILEIPLFSKSKVDRKIFNHFIQPAIRTYKQLGDSLNIFANPTLDQLKQNRYYVLGYPFLKNKVSSLFLNQTGKKKGIFRRKYTNIP", "aa_hexdigest": "085eef393f2354cee99c2c57d0c11b25", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 701920, "stop": 702693, "observations": { "mutation": [ 701946, 702462, 702591 ], "stop": [ 701946, 702462, 702591 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W8P6", "query_cov": 1.0, "subject_cov": 0.4901315789473684, "identity": 0.993, "score": 297.0, "evalue": 1.06e-99, "gene_start": 1, "gene_end": 149, "reference_start": 148, "reference_end": 296, "reference_sequence": "MIVAFKRLGFLASIASLTVLSACAAVPLEKWDQFYVSNIPSSGSIHRANYDLTFALTFTNKTTNNGQDNFSAFSGTGWLIDWKGSGGGLNEPFRVYLATNLHVAAVLKNQQDYDPYKNSDTSADWTTAFRIGKYTHVRDFVEPSQVKLPNNAQAFVNVQTTVIPKTAFAARDFVDYSFPQEQKDKEKRKQQWVKNSHTKNSDVQPFAEFAILEIPLFSKSKVDRKIFNHFIQPAIRTYKQLGDSLNIFANPTLDQLKQNRYYVLGYPFLKNKVSSLFLNQTGKKKGIFRRKYTNIPWKTSYYLN" }, "paralog": false, "identity": 0.9883268482490273, "score": 514.0, "evalue": 1.18e-187, "description": "Internal stop codon at 701946, 702462, 702591. Nonsense mutation around 701946, 702462, 702591.", "psc": { "uniref90_id": "UniRef90_A0AB38W8P6", "product": "Lipoprotein", "uniref50_id": "UniRef50_P75195", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W8P6", "UniRef:UniRef50_P75195" ] }, "pscc": { "uniref50_id": "UniRef50_P75195", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75195" ], "product": "Uncharacterized lipoprotein MPN_585" } }, "id": "GIHJHMOALN_1237", "locus": "GCA009939745_01237" }, { "type": "cds", "sequence": "contig_1", "start": 702589, "stop": 702834, "strand": "-", "frame": 1, "gene": null, "product": "DUF31 family putative serine protease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00143DB747", "UniRef:UniRef90_UPI00143DB747" ], "nt": "ATGATTGTGGCTTTCAAGCGCCTTGGTTTTCTCGCTAGCATAGCTTCCTTAACTGTATTAAGTGCTTGTGCTGCAGTGCCACTAGAAAAGTGGGATCAGTTCTATGTAAGTAATATTCCTAGTAGTGGTTCGATTCACCGTGCCAACTATGATTTAACCTTTGCTTTAACTTTTACTAATAAAACTACTAACAATGGGCAAGATAACTTTAGCGCCTTTTCTGGCACTGGCTGGCTCATTGATTGA", "aa": "MIVAFKRLGFLASIASLTVLSACAAVPLEKWDQFYVSNIPSSGSIHRANYDLTFALTFTNKTTNNGQDNFSAFSGTGWLID", "aa_hexdigest": "27a4ae5b6919c157144c26f8a16e0504", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00143DB747", "query_cov": 0.9629629629629629, "subject_cov": 0.896551724137931, "identity": 1.0, "score": 158.0, "evalue": 5.08e-49, "valid": true, "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_UPI00143DB747" }, "pscc": { "uniref50_id": "UniRef50_UPI00143DB747", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00143DB747" ], "product": "DUF31 family putative serine protease" }, "genes": [], "id": "GIHJHMOALN_1238", "locus": "GCA009939745_01238" }, { "type": "cds", "sequence": "contig_1", "start": 702898, "stop": 703053, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTATGATTTAATCTTTGGTAATAAAAATACCACAAAATACTATGCTCAGTTTGCCAAAGAACACAAAACCCATTTATACGAGCAAATTAAGCAAATTAACAATGATGAATTCCGCTTTGTCGAAAAGACTAAAAGGCATAACTTGGGGTAA", "aa": "MAYDLIFGNKNTTKYYAQFAKEHKTHLYEQIKQINNDEFRFVEKTKRHNLG", "aa_hexdigest": "282fc9ff7f7dc1613d642b8764a0caa5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6222.954599999999, "isoelectric_point": 9.347158241271973 }, "id": "GIHJHMOALN_1239", "locus": "GCA009939745_01239" }, { "type": "cds", "sequence": "contig_1", "start": 703090, "stop": 703305, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_584", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "SO:0001217", "UniRef:UniRef50_P75196", "UniRef:UniRef90_P75196" ], "nt": "TTGAACTATGATCTCTCCCAGAAATTTAAGGTTTATCTTGAGTTTTCGGGCAAAAAGTACCGAATCTATGGCCAAAGTATAGGCATTAGTCATCTTGATTTAGGCGGTGGATCTTCTGGTTCTTTGTTGTTAAATGATAAGAAACAAATCGCTGGCATTTACTTTGGCGTTGATGGTGCTAACAATGAATTAGGCTTAGCACAGTTATTACGCTGA", "aa": "MNYDLSQKFKVYLEFSGKKYRIYGQSIGISHLDLGGGSSGSLLLNDKKQIAGIYFGVDGANNELGLAQLLR", "aa_hexdigest": "1aa1fda7670c3abf5a06f733d472adf5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 702898, "stop": 703305, "observations": { "mutation": [ 703092 ], "stop": [ 703092 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75196", "query_cov": 1.0, "subject_cov": 0.5259259259259259, "identity": 1.0, "score": 141.0, "evalue": 5.23e-42, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MNYDLSQKFKVYLEFSGKKYRIYGQSIGISHLDLGGGSSGSLLLNDKKQIAGIYFGVDGANNELGLAQLLRWKPETYHNDEARSVAYDLIFGNKNTTKYYAQFVKEHKTHLYEQIKQINNDEFRFVEKTKRHNLG" }, "paralog": false, "identity": 0.9777777777777777, "score": 271.0, "evalue": 2.26e-95, "description": "Internal stop codon at 703092. Nonsense mutation around 703092.", "psc": { "uniref90_id": "UniRef90_P75196", "product": "Uncharacterized protein MPN_584", "uniref50_id": "UniRef50_P75196", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75196", "UniRef:UniRef50_P75196", "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_P75196", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75196" ], "product": "Uncharacterized protein MPN_584" } }, "id": "GIHJHMOALN_1240", "locus": "GCA009939745_01240" }, { "type": "cds", "sequence": "contig_1", "start": 703391, "stop": 703810, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_585", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75195", "UniRef:UniRef90_P75195" ], "nt": "GTGCAAACAAGTGTAATTCCCAAAACCGCTTTTGCTGCTAGGGACTTTGTGGATTACAGTTTTCCTCAAGAACAAAAAGATAAAGAAAAGAGAAAACAACAATGGGTGAAAAACAGTCATACTAAGAATTCTGATGTCCAGCCTTTTGCTGAGTTTGCAATTTTAGAAATTCCCTTATTTTCGAAAAGTAAAGTAGATCGCAAAATCTTTAATCACTTTATTCAACCAGCCATACGCACTTATAAACAGTTAGGTGATAGCCTTAACATTTTTGCTAACCCCACACTGGATCAATTAAAACAAAATCGCTATTATGTGTTAGGTTATCCTTTTTTAAAAAATAAAGTGTCATCACTATTTCTTAACCAAACGGGAAAAAAGAAAGGAATATTTAGGCGAAAATACACAAATATCCCATGA", "aa": "MQTSVIPKTAFAARDFVDYSFPQEQKDKEKRKQQWVKNSHTKNSDVQPFAEFAILEIPLFSKSKVDRKIFNHFIQPAIRTYKQLGDSLNIFANPTLDQLKQNRYYVLGYPFLKNKVSSLFLNQTGKKKGIFRRKYTNIP", "aa_hexdigest": "db4ef5465f033749355a44ef45576c3d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 703367, "stop": 704110, "observations": { "mutation": [ 703393, 703909, 704038, 704050 ], "stop": [ 703393, 703909, 704038, 704050 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75195", "query_cov": 1.0, "subject_cov": 0.4602649006622517, "identity": 0.993, "score": 276.0, "evalue": 1.5e-91, "gene_start": 1, "gene_end": 139, "reference_start": 156, "reference_end": 294, "reference_sequence": "MLVVFKRLGFIVSIFSLTFLSACAAFDKWQEFYINNIPSSTEIHRFNYDLNFSLTFTNRITSNGEAKFSVTYGTGWLIDWKEPDKKKENDPFRAYLATNLHVAASLINPQDYEPYKNKDDAGWTTTFRLGKYTKVSDFVSPNQFGLPNAAQALVNVQTSVIPKTAFAARDFVDYSFPQEQKDKEKRKQQWVKNSHTKNSDVQPFAEFAILEIPLFSKSKVDRKIFNHFIQPAIRTYKQLGDSLNIFANPTLDQLKQNRYYVLGYPFLKNKVSSLFLNQTGKKKGIFRRKYTNIPWKTSYYLN" }, "paralog": false, "identity": 0.979757085020243, "score": 484.0, "evalue": 3.91e-176, "description": "Internal stop codon at 703393, 703909, 704038, 704050. Nonsense mutation around 703393, 703909, 704038, 704050.", "psc": { "uniref90_id": "UniRef90_P75195", "product": "Uncharacterized lipoprotein MPN_585", "uniref50_id": "UniRef50_P75195", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75195", "UniRef:UniRef50_P75195", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75195", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75195" ], "product": "Uncharacterized lipoprotein MPN_585" } }, "id": "GIHJHMOALN_1241", "locus": "GCA009939745_01241" }, { "type": "cds", "sequence": "contig_1", "start": 704048, "stop": 704275, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGTTGTTTTCAAGCGTCTTGGTTTTATTGTTAGCATATTCTCTTTAACTTTTTTAAGTGCTTGTGCAGCCTTTGATAAGTGGCAAGAGTTTTACATTAACAATATCCCTAGTAGTACGGAAATCCACCGATTCAACTATGACTTAAACTTCTCGCTTACTTTTACTAATAGAATCACTAGCAACGGTGAAGCTAAATTTAGTGTCACATATGGTACTGGTTGA", "aa": "MLVVFKRLGFIVSIFSLTFLSACAAFDKWQEFYINNIPSSTEIHRFNYDLNFSLTFTNRITSNGEAKFSVTYGTG", "aa_hexdigest": "a220d5ac26ea612ca60edbad7584f6ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8588.689499999993, "isoelectric_point": 7.907579231262207 }, "id": "GIHJHMOALN_1242", "locus": "GCA009939745_01242" }, { "type": "cds", "sequence": "contig_1", "start": 704924, "stop": 705262, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5NA61", "UniRef:UniRef90_A0AAV5NA61" ], "nt": "GTGCAAGCCCTTGTTAGAGCCCAAACCACGATAATTCCCAAGACGGCTTTTGCTGCTCGGGACTTTGTTAATTACAGTTTTACTAAAGAACAAAAAGATAATCAAAAGGAAGAATATCAATGGATTAAAGACAGTAAGACTAAGGATTCTGATACCCGGTCTTACTCTGATTTTGCGGTCTTAGAAATTCCCTTATTTCTGGATAATCCAACAGATCGCAAAATCTTTGATCACTTTATTCAACCAGCCATACGCACTTATAAACAGTTAGGTGATAGCCTTAACATTTTTGCTAACCCCACACTGGATCAATTAAAAAAAATCGCTATTATGCGTTAG", "aa": "MQALVRAQTTIIPKTAFAARDFVNYSFTKEQKDNQKEEYQWIKDSKTKDSDTRSYSDFAVLEIPLFLDNPTDRKIFDHFIQPAIRTYKQLGDSLNIFANPTLDQLKKIAIMR", "aa_hexdigest": "2f39ba38dacb9d1f49d229b2465fce31", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 704902, "stop": 705523, "observations": { "insertion": [ 704947 ], "mutation": [ 705343, 705469, 705481 ], "stop": [ 705343, 705469, 705481 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAV5NA61", "query_cov": 0.9553571428571429, "subject_cov": 0.5194174757281553, "identity": 0.981, "score": 209.0, "evalue": 6.25e-67, "gene_start": 1, "gene_end": 107, "reference_start": 88, "reference_end": 194, "reference_sequence": "MTKLIFIATTYGTGWLIDWKEPDKKKENEPFRAYLATNLYVAASLINPQDYEAYKDTVLGWTTTFRLGKYTNVKDFVAPHQFGLPNAVQALVRAQTTIIPKTAFAARDFVNYSFTKEQKDNQKEEYQWIKDSKTKDSDTRSYSDFAVLEIPFFLDNPTDRKIFDHFIQPAIRTYKQLGDSLNIFANPTLDQLKKSLLCVRLSVFKK" }, "paralog": false, "identity": 0.9805825242718447, "score": 397.0, "evalue": 1.55e-143, "description": "Internal stop codon at 705343, 705469, 705481. Frameshift due to insertion around 704947. Nonsense mutation around 705343, 705469, 705481.", "psc": { "uniref90_id": "UniRef90_A0AAV5NA61", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAV5NA61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAV5NA61", "UniRef:UniRef50_A0AAV5NA61" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAV5NA61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5NA61" ], "product": "Lipoprotein" } }, "id": "GIHJHMOALN_1243", "locus": "GCA009939745_01243" }, { "type": "cds", "sequence": "contig_1", "start": 705471, "stop": 705707, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HPD2", "UniRef:UniRef90_A0AB33HPD2" ], "nt": "TTGGAAGCGTTTTGGTTTTCTTGGTGGCGTATTGGCACTAACCTTTTTAAATGCTTGTGCAACTTTAAACTTCCCGATAAATTTCGTGATTTTTACATTAATAACATTCCTGGTAGTAGCGAAGTGCACCGCGCTAACTATGACCTAAGCTTTTCCTTAACCTTTACTAATAAGTACATTAATAATGACAAAACTTATATTCATAGCTACTACATATGGTACTGGTTGACTTATTGA", "aa": "MEAFWFSWWRIGTNLFKCLCNFKLPDKFRDFYINNIPGSSEVHRANYDLSFSLTFTNKYINNDKTYIHSYYIWYWLTY", "aa_hexdigest": "24db73c19566b8d618066ddce2107fdd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 705360, "stop": 705707, "observations": { "mutation": [ 705371, 705440, 705458, 705473 ], "stop": [ 705371, 705440, 705458, 705473 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HPD2", "query_cov": 0.9102564102564102, "subject_cov": 0.6173913043478261, "identity": 1.0, "score": 157.0, "evalue": 2.14e-48, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MEAFWFSWWRIGTNLFKCLCNFKLPDKFRDFYINNIPGSSEVHRANYDLSFSLTFTNKYINNDKTYIHSYYIWYWLTYWLKGTWQKEGEWTLPRLFSHELIRSSIINKSTRLWSL" }, "paralog": false, "identity": 0.8956521739130435, "score": 212.0, "evalue": 1.8e-72, "description": "Internal stop codon at 705371, 705440, 705458, 705473. Nonsense mutation around 705371, 705440, 705458, 705473.", "psc": { "uniref90_id": "UniRef90_A0AB33HPD2", "uniref50_id": "UniRef50_A0AB33HPD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HPD2", "UniRef:UniRef50_A0AB33HPD2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HPD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HPD2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9719.912499999995, "isoelectric_point": 8.581338310241701 }, "id": "GIHJHMOALN_1244", "locus": "GCA009939745_01244" }, { "type": "cds", "sequence": "contig_1", "start": 705721, "stop": 705867, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTTACGATTTAATCTTTGGTAACAGTAACACAAAACACTTTTATGCTCAGTTTGCCAAACAACAAAAGACCCATTTGTACGAGCAAATTACTAAAACCCGCGACCCGCAATTCCGTTTTGTGGAGAAAGCTAAAAAGAATTAG", "aa": "MAYDLIFGNSNTKHFYAQFAKQQKTHLYEQITKTRDPQFRFVEKAKKN", "aa_hexdigest": "ec97a6c703e14b988a2a24c6d97f3145", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5811.545000000001, "isoelectric_point": 9.821838951110838 }, "id": "GIHJHMOALN_1245", "locus": "GCA009939745_01245" }, { "type": "cds", "sequence": "contig_1", "start": 705901, "stop": 706104, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "GTGGCTGGTTTTCAATTTGGCCAAAATTTTCAAGGACGTCATTATCAACAATATGGCAAGGGAATTGTTTTAAACCATGCTGGTTTTACTGGTGGAGTTTCTGGTACCGTTGTTTTAAATGATCTGAAAAAAATTGCTGGCATTTACTTTGCTACCCATTTCACTGCCAATGGTGATTGGGGGGTAATCCAATTATTGCGTTGA", "aa": "MAGFQFGQNFQGRHYQQYGKGIVLNHAGFTGGVSGTVVLNDLKKIAGIYFATHFTANGDWGVIQLLR", "aa_hexdigest": "693e1a76ed33e3d518d2dfa52bdb3e72", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 44, "aa_cov": 0.6567164179104478, "hmm_cov": 1.0, "evalue": 2.1777515486502207e-11, "score": 39.47071838378906, "start": 8, "stop": 51 } ], "seq_stats": { "molecular_weight": 7317.221600000001, "isoelectric_point": 9.696576881408692 }, "id": "GIHJHMOALN_1246", "locus": "GCA009939745_01246" }, { "type": "cds", "sequence": "contig_1", "start": 706117, "stop": 706470, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGCCACTTTTTCTTGATAAAACCACTGATCGCAAAATCTTTAATCACTTTATTCAACCGGCCATTAATACTTATAAACAGTTAGGTGATAGTTTAAACATCTTTGCCAATCCAACCTTAGACCAGTTAAAGCAAAACCGCTATTATGTACTTGGTTATCCCAATTTAAGAAATAGTGTTAGAACTTTACTGGTAAACCAAACTAAAGCAAGCATAGCAGCAGCTAAAACAATACCTCAGCAAACAACGCAGGAAAGTAAAACTGAAAAACCCTTTGTTGTGGGTAAGAGTACGGTTAAACCTACCTTAATTAGAAATAAGGGCGATAAGTTTAATGGTAGCACAGTTGCTTGA", "aa": "MPLFLDKTTDRKIFNHFIQPAINTYKQLGDSLNIFANPTLDQLKQNRYYVLGYPNLRNSVRTLLVNQTKASIAAAKTIPQQTTQESKTEKPFVVGKSTVKPTLIRNKGDKFNGSTVA", "aa_hexdigest": "23907416ca35fdf332ef145e622db57b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 103, "aa_cov": 0.7435897435897436, "hmm_cov": 0.912621359223301, "evalue": 5.041266800511591e-11, "score": 38.271968841552734, "start": 4, "stop": 90 } ], "seq_stats": { "molecular_weight": 13170.98339999998, "isoelectric_point": 10.10234088897705 }, "id": "GIHJHMOALN_1247", "locus": "GCA009939745_01247" }, { "type": "cds", "sequence": "contig_1", "start": 706534, "stop": 706815, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGCACTTAGGTGATAATCTTCAAACTTTAGGTAACTATTCACCTTACAATAAATTAGATCCTGAATATGGTGTTACCAAAGCTTTTAGACTTGCTAAATACACAAGTGTCAAAGATTTTGTTTTACCTAGTCAATTAGGCTTACCTAATGACGCCAAAGCTTTTGTAACTGCGCAACCAACGGTGCTACCCAAAACAGCTTTTACTTCCAGGGATTTTGTCGATTACAGTTTGCCCAAAGATCAAGTAGATAAAAAAGAAGAATTAACAGATTTACATTGA", "aa": "MHLGDNLQTLGNYSPYNKLDPEYGVTKAFRLAKYTSVKDFVLPSQLGLPNDAKAFVTAQPTVLPKTAFTSRDFVDYSLPKDQVDKKEELTDLH", "aa_hexdigest": "c387eb4cfcd9141c9c92542c4fc88795", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 89, "aa_cov": 0.9032258064516129, "hmm_cov": 0.9887640449438202, "evalue": 4.351430989982152e-08, "score": 28.616724014282227, "start": 2, "stop": 85 } ], "seq_stats": { "molecular_weight": 10463.707699999994, "isoelectric_point": 6.040131950378417 }, "id": "GIHJHMOALN_1248", "locus": "GCA009939745_01248" }, { "type": "cds", "sequence": "contig_1", "start": 706885, "stop": 707121, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTGGCCTTCAAGCGTTTTGGTTTTCTAGCTAGCATAGCCTCTTTAACTCTTTTAAACGCTTGTGCAACAGTTCAATTACCTAATCAATTTGAAGGGTTTTACATCAATAATATTCCCAATAGTACGGAAATCCACCGTGCTAATTACGATTTAGTTTTTTCCTTGGACTTTACTAATAGATATAACCTTGAAAACAAAACTAAAGATTTTGCAATTTATGGCAGTGGTTGA", "aa": "MALAFKRFGFLASIASLTLLNACATVQLPNQFEGFYINNIPNSTEIHRANYDLVFSLDFTNRYNLENKTKDFAIYGSG", "aa_hexdigest": "4df87be822e10026f44d383f4f71d68d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8802.869899999994, "isoelectric_point": 6.526446342468262 }, "id": "GIHJHMOALN_1249", "locus": "GCA009939745_01249" }, { "type": "cds", "sequence": "contig_1", "start": 707338, "stop": 707436, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI001004E809", "UniRef:UniRef100_A0AB38W5Z1", "UniRef:UniRef50_A0AB38W5Z1", "UniRef:UniRef90_A0AB38W5Z1" ], "nt": "ATGCTCAAAGCTTCATCATTGCTCTTCTTCCGTGGTGTTCAGCGTAACAGTTACGCTAAACCCCAAGCCTGTTTAGAAGAACGGGTAGTAGCAAAGTAG", "aa": "MLKASSLLFFRGVQRNSYAKPQACLEERVVAK", "aa_hexdigest": "e6639d7445395d01b147bc219ef44ca9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001004E809", "uniref100_id": "UniRef100_A0AB38W5Z1", "db_xrefs": [ "SO:0001217", "UniParc:UPI001004E809", "UniRef:UniRef100_A0AB38W5Z1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W5Z1", "uniref90_id": "UniRef90_A0AB38W5Z1" }, "psc": { "uniref90_id": "UniRef90_A0AB38W5Z1", "uniref50_id": "UniRef50_A0AB38W5Z1" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5Z1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5Z1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3641.2713999999996, "isoelectric_point": 10.024334144592284 }, "id": "GIHJHMOALN_1250", "locus": "GCA009939745_01250" }, { "type": "cds", "sequence": "contig_1", "start": 707388, "stop": 707507, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "GTGCATGCTGATTTTAGTGGTGGTGTGTCAGGTGCATTAGTCTTAAATGACCAAAAGCAAATTGTGGGAATCTACTTTGCTACTACCCGTTCTTCTAAACAGGCTTGGGGTTTAGCGTAA", "aa": "MHADFSGGVSGALVLNDQKQIVGIYFATTRSSKQAWGLA", "aa_hexdigest": "3fbd61b5b57fffc00bfcb80c34e2a1f3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 26, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 4.659305024829756e-08, "score": 28.519092559814453, "start": 2, "stop": 27 } ], "seq_stats": { "molecular_weight": 4125.620600000001, "isoelectric_point": 8.27479114532471 }, "id": "GIHJHMOALN_1251", "locus": "GCA009939745_01251" }, { "type": "cds", "sequence": "contig_1", "start": 707592, "stop": 707918, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SSS5", "UniRef:UniRef90_A0AAX0SSS5" ], "nt": "TTGGATAATCCCACTGATCGCAACATTTTTGATCATTTGATCCAACCAGCCATACGCACTTATAAACAGTTAGGTGATAGTTTAAACATCTTTGTTGATAACCCAATTAATCAGCTCAAGAACCACAGCTATTATCTATTGGGTTATCCCAACTTACAGAGCAAGGTGAAAACCTTACTAATTAACCAAACTGGTAAAAAACAAATAAAACCCGAACAGATCCTGCAACAAACTAACCAAAACCGCTTTTTATTGTATTCAGGACGTGGTACACCCAACTTAATTAGCACTAAAGGTGCAACTTTCAATCAAAGTGAATTTGCTTGA", "aa": "MDNPTDRNIFDHLIQPAIRTYKQLGDSLNIFVDNPINQLKNHSYYLLGYPNLQSKVKTLLINQTGKKQIKPEQILQQTNQNRFLLYSGRGTPNLISTKGATFNQSEFA", "aa_hexdigest": "f64faec635a68added260c134e693053", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 707388, "stop": 708158, "observations": { "mutation": [ 707594, 707996 ], "stop": [ 707594, 707996 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAX0SSS5", "query_cov": 1.0, "subject_cov": 0.421875, "identity": 0.991, "score": 217.0, "evalue": 1.17e-69, "gene_start": 1, "gene_end": 108, "reference_start": 81, "reference_end": 188, "reference_sequence": "MGKYTDVKQFVLPSKLGLPNFEQAFVFTQLAVIPKTAFLARDFFDYTLPKDAKAWKQGNQSDKTVTHPYADFAVLELPLFLDNPTDRNIFDHLIQPAIRTYKQLGDSLNIFVDNPINQLKNHSYYLLGYPNLQSKVKTLLINQTGKKQIKPEQILQQTNQNRFLLYSGRGTPNLISTKGATFNQSEFAWNYEHSKSFKFKHQYTYKQYQAYGKGLAVVHADFSGGVSGALVLNDQKQIVGIYFATTRSSKQAWGLA" }, "paralog": false, "identity": 0.98828125, "score": 510.0, "evalue": 1.35e-187, "description": "Internal stop codon at 707594, 707996. Nonsense mutation around 707594, 707996.", "psc": { "uniref90_id": "UniRef90_A0AAX0SSS5", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AAX0SSS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAX0SSS5", "UniRef:UniRef50_A0AAX0SSS5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SSS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SSS5" ], "product": "Lipoprotein" } }, "id": "GIHJHMOALN_1252", "locus": "GCA009939745_01252" }, { "type": "cds", "sequence": "contig_1", "start": 707994, "stop": 708158, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCAAATACACCGATGTAAAGCAGTTTGTATTACCAAGCAAATTAGGTTTACCCAATTTTGAGCAAGCCTTTGTCTTTACCCAACTTGCTGTAATTCCCAAAACGGCTTTTTTAGCGAGAGACTTTTTCGATTACACCTTGCCTAAAGATGCTAAAGCATGA", "aa": "MGKYTDVKQFVLPSKLGLPNFEQAFVFTQLAVIPKTAFLARDFFDYTLPKDAKA", "aa_hexdigest": "3fe7e6e7347edc116ed1bd7d0fa092e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6128.098500000002, "isoelectric_point": 9.15762119293213 }, "id": "GIHJHMOALN_1253", "locus": "GCA009939745_01253" }, { "type": "cds", "sequence": "contig_1", "start": 708617, "stop": 708808, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAATCAAACTGGTGAAAACTCAGCTGTGGCTTACGACTTAATTTTTGGTAATAAGGACACCACCAAGTACTACGCCCAATTTGCTAAGAAAAACAGTACCCATTTTTACAAGCAAATTGAACAAGCAAAGGATCAAAGTTTGAAATACGTACAAAGTGATAAGACAACGGATGTTAAAAACTCTTAA", "aa": "MGNQTGENSAVAYDLIFGNKDTTKYYAQFAKKNSTHFYKQIEQAKDQSLKYVQSDKTTDVKNS", "aa_hexdigest": "554d358d99d933180cda197b490be490", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7188.818600000004, "isoelectric_point": 8.892591667175292 }, "id": "GIHJHMOALN_1254", "locus": "GCA009939745_01254" }, { "type": "cds", "sequence": "contig_1", "start": 708815, "stop": 708928, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCGCTTCTGGTTCTTTAGTGCTCAATGACAAGAAACAGATTGCCGGCATTTACTTTGGTACAACTTATTCGGACGTTGATGCTTGGGGAGTAGCCCAATTGTTGCGTTGA", "aa": "MGASGSLVLNDKKQIAGIYFGTTYSDVDAWGVAQLLR", "aa_hexdigest": "e472c169d777e1313afbe2f18f9b9d84", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3946.4412999999986, "isoelectric_point": 5.793905448913574 }, "id": "GIHJHMOALN_1255", "locus": "GCA009939745_01255" }, { "type": "cds", "sequence": "contig_1", "start": 709031, "stop": 709360, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGGAGAATCAAAATGATAAAAAGGTTTTTGATCACTTCATCGAGCCTGCCATGAGAACTTATAAGCTTTTAGGTGATAGCTTAAATCTATTCGCTAAACAATCACTAGATGAACTGAAAGGTAACCATTATTATTTGGCAGGTTATCCTTATCTAAAAAATAAGGTTAGATCTTTGTTTATTAACCAAACAGATAAAACCAAAAAAGAAGACGACCAAATAGTACAGCACAGTAACATCGACAAACCGTTTGATATTCGTCCTTCTTTGATGAAACCAACCTTAATTCGCAATAAAGGCGATGATTTTAATGACAGTACATTGGCATGA", "aa": "MENQNDKKVFDHFIEPAMRTYKLLGDSLNLFAKQSLDELKGNHYYLAGYPYLKNKVRSLFINQTDKTKKEDDQIVQHSNIDKPFDIRPSLMKPTLIRNKGDDFNDSTLA", "aa_hexdigest": "d17e9a1429be359582ab8ccb8a4bce86", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 104, "aa_cov": 0.7798165137614679, "hmm_cov": 0.9903846153846154, "evalue": 1.0568244896412925e-10, "score": 37.214847564697266, "start": 3, "stop": 87 } ], "seq_stats": { "molecular_weight": 12738.292799999981, "isoelectric_point": 7.913123512268066 }, "id": "GIHJHMOALN_1256", "locus": "GCA009939745_01256" }, { "type": "cds", "sequence": "contig_1", "start": 709496, "stop": 709720, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGCCAAGTAAAGTCAATTTAGCGAACAATGCCCAAGTCTTTGTCAATGTGCAAACAACGGTAATTCCCAAAACTGCTTTTACTGCTAGAGATTTTGTGAATTACAAATTGTCAGCAGATGAAGTAACAAAACAAAAGAAGCAATTACAAGAACTTATCGAAAAAGCACAAAAAGGAGAAGTACAAGAAGGACAACAAACTGACACGCAAGCAATTCAATGA", "aa": "MLPSKVNLANNAQVFVNVQTTVIPKTAFTARDFVNYKLSADEVTKQKKQLQELIEKAQKGEVQEGQQTDTQAIQ", "aa_hexdigest": "5e686c5cb8fe03bded4a2f2bf11638a4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8292.305400000001, "isoelectric_point": 8.008730125427245 }, "id": "GIHJHMOALN_1257", "locus": "GCA009939745_01257" }, { "type": "cds", "sequence": "contig_1", "start": 709901, "stop": 710149, "strand": "-", "frame": 3, "gene": null, "product": "DUF31 family putative serine protease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00143DB747" ], "nt": "ATGTTGGTTGCTTTTAAACGTTTCGGTTTCATTTTTAGCATAGGTGGCTTGGCCTTTTTAAGTGCTTGTGCTGCTTTTAAGCCTGATAAATTTGAAGGGTTTTACGTTAATAATATTCCCAGTAGTGCAGAAATACACCGCACTAACTATGATCTTAGTTTTGCCTTAACTTTTACTAACAAGTTTAACGGTAAAAATAATCAAACAGAATTCATTGCTACCTATGGTACTGGTTGGTTAATTGACTGA", "aa": "MLVAFKRFGFIFSIGGLAFLSACAAFKPDKFEGFYVNNIPSSAEIHRTNYDLSFALTFTNKFNGKNNQTEFIATYGTGWLID", "aa_hexdigest": "5207196de483e0adb325bc7ea3a2b7b7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00143DB747", "query_cov": 0.975609756097561, "subject_cov": 0.9195402298850575, "identity": 0.625, "score": 95.9, "evalue": 2.67e-24, "valid": false, "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_UPI00143DB747" }, "pscc": { "uniref50_id": "UniRef50_UPI00143DB747", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00143DB747" ], "product": "DUF31 family putative serine protease" }, "genes": [], "id": "GIHJHMOALN_1258", "locus": "GCA009939745_01258" }, { "type": "cds", "sequence": "contig_1", "start": 710225, "stop": 710368, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTGTATGATCTCATCTTTGGCAATCCAAACACGAGAAAATATTACGCCCAGTTTGCAAAGAAAAATAACACCCACTTGTACAAACAAATTGTTAAAAGTAGGAGCGCTGAGTTCCGTTTTGTGGAAAACTCCAATAACTAA", "aa": "MVYDLIFGNPNTRKYYAQFAKKNNTHLYKQIVKSRSAEFRFVENSNN", "aa_hexdigest": "70841bd54826ece1adbecd09ff9cb6eb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5646.312500000001, "isoelectric_point": 9.89874973297119 }, "id": "GIHJHMOALN_1259", "locus": "GCA009939745_01259" }, { "type": "cds", "sequence": "contig_1", "start": 710429, "stop": 710578, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGGGTAAAGATTACCAAATCTATGGCAAAGGCATAGGGATTAATCGTAGTGATTTGGGTGGTGGCGCTTCTGGATCTTTAGTTTTAAACGATAAAAAGCAGATTGCCGGTATTTATTTTGGTGCCAGTAGTTCTGGGCCAAATGAGTGA", "aa": "MGKDYQIYGKGIGINRSDLGGGASGSLVLNDKKQIAGIYFGASSSGPNE", "aa_hexdigest": "034b00dfe4c04d429f6478461a8b4fa5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 39, "aa_cov": 0.7959183673469388, "hmm_cov": 1.0, "evalue": 4.5209714016300204e-13, "score": 45.00447463989258, "start": 3, "stop": 41 } ], "seq_stats": { "molecular_weight": 4992.4908, "isoelectric_point": 8.064430809020994 }, "id": "GIHJHMOALN_1260", "locus": "GCA009939745_01260" }, { "type": "cds", "sequence": "contig_1", "start": 710627, "stop": 711220, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MPN_583", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197" ], "nt": "GTGGATTTTAGACTGGGCAAATATACTAACGTGAAAGACTTTGTGTCACCAAACGAAATAGGATTACCTAATGGTGCACAAGCCTTTGTATCAGCGCAGACAAGCACAATTCCCAAAACGGCTTTCACTGCGCACGATTTTGTGGATTACACCTTGCCCCAAGAACAAAAAGGTAAAGAACAAAAGGAGCAGCAGTGGATGAAGAACAGTCAAACTAAGGATACTGATGTTCATCCCTATGCTGATTTTGCAGTCTTAGAAATCCTTTTGTTTCCTGACAGTTCTACTGATCGCAAAATTTTTAATCACTTTATCCAACCTGCCATTAGAGCCTATAAACAATTAGGTGATAGCTTAAATATCTTTGCTAATCAAACCCTAGATCAACCTAAACATAACCGTTATTACTTGTTGGGTTATCCTTTTTTAAGAGAAAGGGGTCAATCTTTATTTGTAAGTCAAACAGGTGAAGAAAAGGAGATTTTGGGTCAAAAGGCACAAGTGCCCAATGAAGACCCCATCGTTTCTTTAAGAGTCCCATCTAAACCTACTTTATCTAGAAATAAGGGTGATGACTTTAATGGTAGTTCTTGA", "aa": "MDFRLGKYTNVKDFVSPNEIGLPNGAQAFVSAQTSTIPKTAFTAHDFVDYTLPQEQKGKEQKEQQWMKNSQTKDTDVHPYADFAVLEILLFPDSSTDRKIFNHFIQPAIRAYKQLGDSLNIFANQTLDQPKHNRYYLLGYPFLRERGQSLFVSQTGEEKEILGQKAQVPNEDPIVSLRVPSKPTLSRNKGDDFNGSS", "aa_hexdigest": "4c99003beebf927c1be2bb591c4115a5", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0006BA639D", "query_cov": 0.8984771573604061, "subject_cov": 0.8762376237623762, "identity": 0.836, "score": 296.0, "evalue": 6.67e-100, "valid": false, "product": "DUF31 family putative serine protease", "uniref50_id": "UniRef50_P75197" }, "pscc": { "uniref50_id": "UniRef50_P75197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75197" ], "product": "Uncharacterized protein MPN_583" }, "genes": [], "id": "GIHJHMOALN_1261", "locus": "GCA009939745_01261" }, { "type": "cds", "sequence": "contig_1", "start": 711347, "stop": 711601, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_587", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75193", "UniRef:UniRef90_P75193" ], "nt": "ATGGTAATTGCCTTAAAGCGGTTTAGTTTTCTAGCTAGCATAGCTACATTAACTGTGCTCAACGCTTGTGCAACAATTTCACTATCTGATAAATCTGGTGAGTTTTACATTAACAATATTCCTAGTAGTGCTGAATTACACCGGATTAACTATGACTTGACCTTCTCTTTAGGTTTTACCAACAGAATTCAGACTAAAAAGAAGAATGAAGTAAACTTCGTTGCCTTTTATGGTACTGGTTGGTTGATTGACTGA", "aa": "MVIALKRFSFLASIATLTVLNACATISLSDKSGEFYINNIPSSAELHRINYDLTFSLGFTNRIQTKKKNEVNFVAFYGTGWLID", "aa_hexdigest": "48ecbadbc1ae91162ddd6413354b574a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 711150, "stop": 711601, "observations": { "deletion": [ 711163 ], "mutation": [ 711226, 711349 ], "stop": [ 711226, 711349 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75193", "query_cov": 1.0, "subject_cov": 0.56, "identity": 0.988, "score": 159.0, "evalue": 1e-48, "gene_start": 1, "gene_end": 84, "reference_start": 1, "reference_end": 84, "reference_sequence": "MVIALKRFSFLASIATLTVLNACATISLSDKSGEFYINNIPSSAELHRINYDLTFSLGFTNRIQTKKKNEVNFVAFYGTGLLIDWKEGNQYQPFRVYLATNVHVAAGLKNEADYLPYSQPDAELGWTVDFRLGKYTNVKDFVSPNENRIT" }, "paralog": false, "identity": 0.98, "score": 285.0, "evalue": 1.43e-100, "description": "Internal stop codon at 711226, 711349. Frameshift due to deletion around 711163. Nonsense mutation around 711226, 711349.", "psc": { "uniref90_id": "UniRef90_P75193", "product": "Uncharacterized lipoprotein MPN_587", "uniref50_id": "UniRef50_P75193", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75193", "UniRef:UniRef50_P75193", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75193", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75193" ], "product": "Uncharacterized lipoprotein MPN_587" } }, "id": "GIHJHMOALN_1262", "locus": "GCA009939745_01262" }, { "type": "cds", "sequence": "contig_1", "start": 711659, "stop": 712105, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "GTGATCCAAGACAAAGGTGTGGTGAACTTTGCTTCCTTTAGCAACTTTACCTTGAAGTACCACGAAAAGCGGTTCCAACAATACGGCTATGGCTTAATGTTGAATGACACTAATTTTCCGGGTGGCTCTTCGGGTAGTCCTTTAATTGGTAAAGACAATAAATTGAACAGCATTTACTTTGGGGTGTTGGAAATTTACCAATCTGGTAGTCTTGCACGCAATGACATTGGGATGAGCCAAATCTTACGTACTCCACAGAACGATAAAGGTTCAAGTATTTCTAAGGGTAGCTATGACTTGATCTTTGGCGATAAGAACACCAAGAATTATTACGCTAAGTTTGCTAAAGATCATCAAACTCATTTGTATCAGAAGATTAAAGAAAGCAAAGACGAACAATTCCGCTTTGTGGAAGCACAAGAGACAACTAATAATTTAGGCAATTAA", "aa": "MIQDKGVVNFASFSNFTLKYHEKRFQQYGYGLMLNDTNFPGGSSGSPLIGKDNKLNSIYFGVLEIYQSGSLARNDIGMSQILRTPQNDKGSSISKGSYDLIFGDKNTKNYYAKFAKDHQTHLYQKIKESKDEQFRFVEAQETTNNLGN", "aa_hexdigest": "aa26f1b80ff1548c4b7791f553cbd8d1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 51, "aa_cov": 0.34459459459459457, "hmm_cov": 0.8823529411764706, "evalue": 5.476556833816668e-15, "score": 51.307559967041016, "start": 11, "stop": 61 } ], "seq_stats": { "molecular_weight": 16789.508199999986, "isoelectric_point": 8.981106758117676 }, "id": "GIHJHMOALN_1263", "locus": "GCA009939745_01263" }, { "type": "cds", "sequence": "contig_1", "start": 712241, "stop": 713254, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_588", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q50339", "UniRef:UniRef90_Q50339" ], "nt": "ATGAGACTGCAATTCAAGTTATTGGGTTTCTTAACACTGCTGGGTACCAGCACTATTCTTAGTGCCTGTGCTGCAACCCAGCCAAACTTTGAACCCAATAACATTGAAGAAAGTGGTCCCATTACACCTACCACACCTACAACTGATGTACCTAAACCAACCGCAGAGGTAGTGCCTGTTAACCGTGGCTTTCACTTCCAAACCAATAAGGTACCTAGTGAAAGTGATGTTTTTAAGCATAACTATGATCTAACTTTTTCGTTGAACTTTACCAATAAGTCTAACGATATCTATGGCACCGGCTGGTTATTTGACTGGAAGGGTGATGAAAAGGCATTAGGTATTGATGGCAGTTTTGTCCCTAGCATTACAAGCAACATTGATAACTCCCTTCTAAAGGATGATCAATTCACCGTTTATTTAGCCACTAACTTACACGTAGCTGATGCTTTAAGGAATGATCAGGATTACGAACCCTACAAAAAAGATCAGAACAAGCAAGATTTTACTGAGAATACTAAAACTGAATTCTTTTCTTTGGGTAAATACTTAGAAGGTGAGCAGTTAAAGCAGTACATTTCTAAAGAAGAAAATCAATCAGCCAATCAAACTGATAAAGCCTTGGTTTCGATTCAGGCCTCCAATATTCCCAAAACGGCTTACACTGCTACTGACTTTGTTGATATGAATAGTTACAGTTATAACAACATCACAACAAGTCTTCCTGGCAACTATGCTGACTTTGCCGTGATTGAAGTTAACCTAAACCTTAAAAATCAACGCGATCAGCAAATTCTTCATGACTTTGTCAAACCAGCAATTAAGGCTTACAAGGCTTTAGGTGATACTTTGGAACTGTTTAGTGCCAAACCACTCAACCAGTTCATCGAACAAAACTACTACTTGTTAGGTTATCCAGTGATTAATAAAGGAAACAATACCGCTAACCTTTTATTAGCGCAACAAAAGAGCTTTGATCACAACAATTCGGATCATAACCAAAAGAGCCAGTGA", "aa": "MRLQFKLLGFLTLLGTSTILSACAATQPNFEPNNIEESGPITPTTPTTDVPKPTAEVVPVNRGFHFQTNKVPSESDVFKHNYDLTFSLNFTNKSNDIYGTGWLFDWKGDEKALGIDGSFVPSITSNIDNSLLKDDQFTVYLATNLHVADALRNDQDYEPYKKDQNKQDFTENTKTEFFSLGKYLEGEQLKQYISKEENQSANQTDKALVSIQASNIPKTAYTATDFVDMNSYSYNNITTSLPGNYADFAVIEVNLNLKNQRDQQILHDFVKPAIKAYKALGDTLELFSAKPLNQFIEQNYYLLGYPVINKGNNTANLLLAQQKSFDHNNSDHNQKSQ", "aa_hexdigest": "cdd50434066eedfcc3904d9d1939c39a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 711938, "stop": 713254, "observations": { "mutation": [ 712243 ], "stop": [ 712243 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50339", "query_cov": 1.002967359050445, "subject_cov": 0.6353383458646616, "identity": 0.997, "score": 654.0, "evalue": 8.66e-234, "gene_start": 1, "gene_end": 337, "reference_start": 1, "reference_end": 338, "reference_sequence": "MRLQFKLLGFLTLLGTSTILSACAATQPNFEPNNIEESGPITPTTPTTDVPKPTAEVVPVNRGFHFQTNNKVPSESDVFKHNYDLTFSLNFTNKSNDIYGTGWLFDWKGDEKALGIDGSFVPSITSNIDNSLLKDDQFTVYLATNLHVADALRNDQDYEPYKKDQNKQDFTENTKTEFFSLGKYLEGEQLKQYISKEENQSANQTDKALVSIQASNIPKTAYTATDFVDMNSYSYNNITTSLPGNYADFAVIEVNLNLKNQRDQQILHDFVKPAIKAYKALGDTLELFSAKPLNQFIEQNYYLLGYPVINKGNNTANLLLAQQKSFDHNNSDHNQKSQWFTKDQSYINKLDRIPVLTNNYRAYNESTGSQLFANQQNESWLNGVVIQDKGVVNFASFSNFTLKYHEKRFQQYGYGLMLNDTNFPGGSSGSPLIGKDNKLNSIYFGVLEIYQSGSLARNDIGMSQILRTPQNDKGSSISKGSYDLIFGDKNTKNYYAKFAKDHQTHLYQKIKESKDEQFRFVETQETTNNLGN" }, "paralog": false, "identity": 0.9954441913439636, "score": 882.0, "evalue": 0.0, "description": "Internal stop codon at 712243. Nonsense mutation around 712243.", "psc": { "uniref90_id": "UniRef90_Q50339", "product": "Uncharacterized lipoprotein MPN_588", "uniref50_id": "UniRef50_Q50339", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50339", "UniRef:UniRef50_Q50339", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q50339", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50339" ], "product": "Uncharacterized lipoprotein MPN_588" } }, "id": "GIHJHMOALN_1264", "locus": "GCA009939745_01264" }, { "type": "cds", "sequence": "contig_1", "start": 713470, "stop": 713847, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_589", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "SO:0001217", "UniRef:UniRef50_Q50338", "UniRef:UniRef90_Q50338" ], "nt": "ATGCCAACACTTACCAAAAACTTAAGGCAAGGTCAACCAGTTGCTGGTAGTAAAGTGCACACATATTACAGTAACGGCTTCCTTAATGAATATTCCTTAAATCAAGGAATTGTTGATTTTGGTACTTTTAAAAGTGCTATCACTGATTATCATGGATATGAATATAAAAACCACGGCTATGGCTTAGCTCTAACTGATACTGATTTACTAGGTGGATCGAGTGGCAGTTTAGTGTTTAACCAAGACAAAAAGATTAGCAGTATTTATTCAGCTGCTACTGAATCAGATAGTGTTGGCTATGCCCAGCTATTGCCTGTTCCTAAAGATGTTAATGGGGTAAGTTTAGTTAAATACAGTTACGACCTTATACTTTGGTGA", "aa": "MPTLTKNLRQGQPVAGSKVHTYYSNGFLNEYSLNQGIVDFGTFKSAITDYHGYEYKNHGYGLALTDTDLLGGSSGSLVFNQDKKISSIYSAATESDSVGYAQLLPVPKDVNGVSLVKYSYDLILW", "aa_hexdigest": "87242a62519f9e884e9deda655e90b23", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 713470, "stop": 713940, "observations": { "mutation": [ 713895 ], "stop": [ 713895 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50338", "query_cov": 1.0, "subject_cov": 0.7961783439490446, "identity": 0.992, "score": 250.0, "evalue": 1.06e-83, "gene_start": 1, "gene_end": 125, "reference_start": 32, "reference_end": 156, "reference_sequence": "MGYPTLWTDDAKLFEWTKTEQRFNHDDFYGSMPTLTKNLRQGQPVAGSKVHTDYSNGFLNEYSLNQGIVDFGTFKSAITDYHGYEYKNHGYGLALTDTDLLGGSSGSLVFNQDKKISSIYSAATESDSVGYAQLLPVPKDVNGVSLVKYSYDLILWW" }, "paralog": false, "identity": 0.9807692307692307, "score": 315.0, "evalue": 7.94e-112, "description": "Internal stop codon at 713895. Nonsense mutation around 713895.", "psc": { "uniref90_id": "UniRef90_Q50338", "product": "Uncharacterized protein MPN_589", "uniref50_id": "UniRef50_Q50338", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50338", "UniRef:UniRef50_Q50338", "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_Q50338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50338" ], "product": "Uncharacterized protein MPN_589" } }, "id": "GIHJHMOALN_1265", "locus": "GCA009939745_01265" }, { "type": "cds", "sequence": "contig_1", "start": 714081, "stop": 714329, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "GTGAATGATTATGAGTTTTTCAATCAATTTGATTACTTTGACGATCCAACTGAATCTTTCACTTTAGGTAAGTTCGTTGATGGTAATGAAATAAAGCAAATAGTTCCTGATGCTATGCATGAACCTAGCTTAGTGCGGATTGAAACTTCAAAATTGCCAAAAACTGCTTACACGGCAACACTTTTTATTAATGATTTGGGGGAATATAGATTACCTGCCGCTGACTTTGCTGTATTGGAATCAATCTAG", "aa": "MNDYEFFNQFDYFDDPTESFTLGKFVDGNEIKQIVPDAMHEPSLVRIETSKLPKTAYTATLFINDLGEYRLPAADFAVLESI", "aa_hexdigest": "8b3e499ae1f978cc3789f34561e0ef49", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 95, "aa_cov": 0.926829268292683, "hmm_cov": 1.0, "evalue": 1.3208184294093466e-07, "score": 27.030988693237305, "start": 5, "stop": 80 } ], "seq_stats": { "molecular_weight": 9402.401499999996, "isoelectric_point": 4.195479393005371 }, "id": "GIHJHMOALN_1266", "locus": "GCA009939745_01266" }, { "type": "cds", "sequence": "contig_1", "start": 714405, "stop": 714626, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGTAAACAACAAAAGTGTTAGTGCAGTTCAACCAGAAGACAAGCAAGCATTAAAACCAGACCCTGCTTTAGGTGAATACACCTCTGACTTAAAGTTCAATACTAACTTCATTCCAAAAAAGGATGATCCCTTTCGTCCCTTTTATGATCTAAGTTTTACGCTCCAGTTTCAAGATCCATATACAGCTACTTACGGTACTGGTTGGCTCATTGATTGA", "aa": "MPVNNKSVSAVQPEDKQALKPDPALGEYTSDLKFNTNFIPKKDDPFRPFYDLSFTLQFQDPYTATYGTGWLID", "aa_hexdigest": "e8bd5c455fb50bb570a386a0bfaa2cff", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8334.204199999996, "isoelectric_point": 4.611427116394043 }, "id": "GIHJHMOALN_1267", "locus": "GCA009939745_01267" }, { "type": "cds", "sequence": "contig_1", "start": 714891, "stop": 715952, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MPN_591", "db_xrefs": [ "GO:0003723", "GO:0003724", "GO:0005524", "RefSeq:WP_010874948.1", "SO:0001217", "UniParc:UPI000013ADAA", "UniRef:UniRef100_Q50336", "UniRef:UniRef50_Q50336", "UniRef:UniRef90_Q50336" ], "nt": "GTGGCTAACAGCTTAAGGAACGTTAATGATTACGATCCTTTTAAATACCGCCCCAGTTATTTTGATCTAGAGGCACCAACCGAATCATTTGCTTTAGGTAAGTTTGTCGATGCGATTGAAGTTAAGCAGATGGCACTAGATGCCTTTAGTGAATCTAGCATGGTCAGTATTGCAACTTCACAGCTACCCAAAACAGCTTACACAGCTACAAGTTTTATTGAAAGCGAGTATTATAGTTTTCCTTATTTATTTAACGACCAAAAATATTACTGGGATTATTTTGATTACAAAATCCCTGCCGCTGACTTTGCCGTATTAGAGTTAGAACTGGATTTAAACAACCAGCAGGATCAGCAAATTAAAGATCATTTCATCGATCCAGCGATCAAGGCTTATAAACAATTAGGTGATAGTACCGGTTTGTTTGCCACCAAACCATTAACTGAATATCAAAACGATACCCATTACCTCTTGGGCTATCCGGTTGTACCTACTGATCATACACAACTGTGGGAATGCAAACAAGGGGCAGAGCGCTTTAGCTATGGTTATTTTTACAGTAATATGGCTAGACTAACCAAAAACCTAAGGCAGGGCGATCCAAACGCTGGTAGTAAGACACACATCGAATACAGCAATGAGTTACTCGATAAGGACTCAATGGATCAAGGTATTGTGCGTTTTAGTACCTTTTTGGGTGCTAACATTAATTACCACGATTACGATTACCGTCAACAGGGTTACGGTTTAACCTTAACCGACACTAACTTGCCAGGTGGATCGAGTGGCAGTTTAGTGTTTAACCAAGACAAAAAGATTAGCAGTATTTATTCAGCTGCTACTGAATCAGATAGTGTTGGCTATGCTCAGTTATTACGAACTCCTAGGGATGTCAACGGTATAAGTGTAGTTTCACAAAGCTATGACCTCATCTTTGGTGATAGCAACACTAAGCGTTACTATGCGATGTTTGCTAAAAAACAGCAAACTCACTTGTACAGTGAAATCCTTAAAAGTACTGATGAACAGTACCGTTATGTGGTAGACAAACAATTCAATTAA", "aa": "MANSLRNVNDYDPFKYRPSYFDLEAPTESFALGKFVDAIEVKQMALDAFSESSMVSIATSQLPKTAYTATSFIESEYYSFPYLFNDQKYYWDYFDYKIPAADFAVLELELDLNNQQDQQIKDHFIDPAIKAYKQLGDSTGLFATKPLTEYQNDTHYLLGYPVVPTDHTQLWECKQGAERFSYGYFYSNMARLTKNLRQGDPNAGSKTHIEYSNELLDKDSMDQGIVRFSTFLGANINYHDYDYRQQGYGLTLTDTNLPGGSSGSLVFNQDKKISSIYSAATESDSVGYAQLLRTPRDVNGISVVSQSYDLIFGDSNTKRYYAMFAKKQQTHLYSEILKSTDEQYRYVVDKQFN", "aa_hexdigest": "e61db966f3ccde618ac15ccaaa1edfdf", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ADAA", "ncbi_nrp_id": "WP_010874948.1", "uniref100_id": "UniRef100_Q50336", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ADAA", "RefSeq:WP_010874948.1", "UniRef:UniRef100_Q50336" ] }, "ips": { "uniref100_id": "UniRef100_Q50336", "uniref90_id": "UniRef90_Q50336" }, "psc": { "uniref90_id": "UniRef90_Q50336", "product": "Uncharacterized protein MPN_591", "uniref50_id": "UniRef50_Q50336", "go_ids": [ "GO:0005524", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_Q50336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50336" ], "product": "Uncharacterized protein MPN_591" }, "genes": [], "id": "GIHJHMOALN_1268", "locus": "GCA009939745_01268" }, { "type": "cds", "sequence": "contig_1", "start": 716475, "stop": 716930, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGGTACAAAATCAAATTAACTATTTACCCTTTGCACAAAATGACCCTAAAGGCGTAATGGACTTTAGCAAGTATTTCAATTACGTTTTTAACTATCACGAAAAGCAATACCAACACCATGGTTATGGTTTATTGTTAGAAGACACTGACTTCCCGGGTGGTTCCAGTGGTAGTCCGTTATTTAACCAAAACAAACAGATTAACAGTATTTACTTTGCGGCTTTACCAAGTAAAAGTTACGGTGTATCGCAAATTCTCCGCGCTACGCAAAACAAAGACAAGTCCAAAAACTACGATTTAATCTTTGGTGATAGCAACACCAAGAAATACTATGCTCAATTTGCTAAGGAGCACAAAACCCACTTGTACCATCAAATTCTTCAAAGTAACGATGAACAGTTCCGCTTTGTCGAAAACGATCAAACTGTAACAAGTCAAACACCATTTAAAAGCTAA", "aa": "MVQNQINYLPFAQNDPKGVMDFSKYFNYVFNYHEKQYQHHGYGLLLEDTDFPGGSSGSPLFNQNKQINSIYFAALPSKSYGVSQILRATQNKDKSKNYDLIFGDSNTKKYYAQFAKEHKTHLYHQILQSNDEQFRFVENDQTVTSQTPFKS", "aa_hexdigest": "cdad11514fddf82b12edb7cacff46c9e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 71, "aa_cov": 0.47019867549668876, "hmm_cov": 0.9154929577464789, "evalue": 1.967879681874661e-16, "score": 56.05778121948242, "start": 3, "stop": 73 } ], "seq_stats": { "molecular_weight": 17603.255599999982, "isoelectric_point": 7.887013816833496 }, "id": "GIHJHMOALN_1269", "locus": "GCA009939745_01269" }, { "type": "cds", "sequence": "contig_1", "start": 717039, "stop": 717482, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "TTGGGTAAATACATTGATGCCCAAACTGTGCAAAAGTTAACTCCAGAGTACCAAAATCTTAAGCACCTCAATAACCGGAATAGTGATGCATTAGTTTCAATTCAAACATCGAAGTTACCAAAAACTGCTTACACTGCCACTGACTTTATCAAAACTGCTCAGTACAAATACAATCACATAGTGGGTAACACAGTTTATGAGTTGGACTTATTCCAAAATGCCGTAAGTTACGCTGACTTTGCGGTGTTGGAATTAGAGTTAAATTTAGCCAACAATCGTGACCAGCAAATCTTTGACAGTTTTATAAATCCAGCGGTAACTGCTTACGAAAAGCTAGGTAACAGTTTGGGTCTCTTTTCCAACCTGCAATTAGATCAGTATGTTGATGATACCCATTATCTATTGGGTTATCCGTTACTTAAACGTGAAAAGACGTCTTACTGA", "aa": "MGKYIDAQTVQKLTPEYQNLKHLNNRNSDALVSIQTSKLPKTAYTATDFIKTAQYKYNHIVGNTVYELDLFQNAVSYADFAVLELELNLANNRDQQIFDSFINPAVTAYEKLGNSLGLFSNLQLDQYVDDTHYLLGYPLLKREKTSY", "aa_hexdigest": "740deee53a82aef4ed3d3f5766ff4a13", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 152, "aa_cov": 0.9659863945578231, "hmm_cov": 0.9473684210526315, "evalue": 1.2834894384227802e-30, "score": 102.7066879272461, "start": 2, "stop": 143 } ], "seq_stats": { "molecular_weight": 16841.695399999997, "isoelectric_point": 5.521191978454589 }, "id": "GIHJHMOALN_1270", "locus": "GCA009939745_01270" }, { "type": "cds", "sequence": "contig_1", "start": 717672, "stop": 718040, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGTTTAAACTCAAGGGTTTTGGTTTTCTCACACTGTTCGCCAGTCAAGCATTTCTAACTGCTTGCTCTGCTACGCTAACAGTAGCTAACACTAATCACAAAAATGAAAGTGATAAGTTTGTGATTTTTGAACCTCAACCACCATTGAGCCAAACTATCCCCAAACCAGAGGCTGAACCAGTTATAGAGCCCGATGCTGTTGCTACACCACCTGTGCAGAATGCCGAGGTGCAAATTAAGCCTGACAGCTCCAAGGGTGTTTACAGTCCTGGTTTTAAGTTCAACACTAACTTTATTCCTAAAGTAAATACTAAGTATCGGCCGGGCTATGATCTTAGCTTTGCCTTAAAGTTTGGTACTAGTTGA", "aa": "MGFKLKGFGFLTLFASQAFLTACSATLTVANTNHKNESDKFVIFEPQPPLSQTIPKPEAEPVIEPDAVATPPVQNAEVQIKPDSSKGVYSPGFKFNTNFIPKVNTKYRPGYDLSFALKFGTS", "aa_hexdigest": "b9906f6d0939d177fcf7279089c58415", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13317.044699999979, "isoelectric_point": 8.709501457214355 }, "id": "GIHJHMOALN_1271", "locus": "GCA009939745_01271" }, { "type": "cds", "sequence": "contig_1", "start": 718141, "stop": 718509, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_593", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010874950.1", "SO:0001217", "UniParc:UPI000013ADB0", "UniRef:UniRef100_Q50334", "UniRef:UniRef50_Q50334", "UniRef:UniRef90_Q50334" ], "nt": "ATGAACAAAAAAGAAAGCACTACCACTAAAAAGCAGTGGTTTAAAAAGTGTTCTTTTAAGAAGTTAAAAGCGGAAATTTGCAACATGTTGCCAACAACGCCGCACAACACCAAGCGCACATTAATCTGGGTGATTGTTTTTAGCTTTATTACTTTTCTTAGTTTTATTTTTGCTTATGTTTGCTTTAACTATGCTCCAGTATCCACCGGTTTCTTGTACTTCTTAGGAGCAGTGTTTTTACTAATTGGCTTTGCCTTTGCTATCTTGAGTTTTGTAGCAATGGTTAAATTTGTGGCTGATTATTTTGCCAATCGTTTTAGTAACACTCAGCTCAAAATGGACTGTGATTGTGCTAAAACGAAAAAGTAA", "aa": "MNKKESTTTKKQWFKKCSFKKLKAEICNMLPTTPHNTKRTLIWVIVFSFITFLSFIFAYVCFNYAPVSTGFLYFLGAVFLLIGFAFAILSFVAMVKFVADYFANRFSNTQLKMDCDCAKTKK", "aa_hexdigest": "e886a167d041086ca03c2e9933835d60", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013ADB0", "ncbi_nrp_id": "WP_010874950.1", "uniref100_id": "UniRef100_Q50334", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013ADB0", "RefSeq:WP_010874950.1", "UniRef:UniRef100_Q50334" ] }, "ips": { "uniref100_id": "UniRef100_Q50334", "uniref90_id": "UniRef90_Q50334" }, "psc": { "uniref90_id": "UniRef90_Q50334", "product": "Uncharacterized protein MPN_593", "uniref50_id": "UniRef50_Q50334", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q50334", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50334" ], "product": "Uncharacterized protein MPN_593" }, "genes": [], "id": "GIHJHMOALN_1272", "locus": "GCA009939745_01272" }, { "type": "cds", "sequence": "contig_1", "start": 718735, "stop": 719037, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_594", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniParc:UPI0001DD643B", "UniRef:UniRef100_P75191", "UniRef:UniRef50_P75191", "UniRef:UniRef90_P75191" ], "nt": "ATGAGAGGGAAAAATAGGAATAAATGCAACAGCATTATTTTTTTAACAGTTGGTTTACTGTTGTTTATAGCAGCTTTAGCGCTTGGTGTATTAGTACTTTTTAACGGGTATCAGGTTAATGTCAATGCTAATGGAGTTGACTTGAAACCCTTTGAGATTGTGCATTTTCCATTTGCGGTCAAAACTTTTCTTTCTGTTTTAACCTTTGTGTTAGCAGCCTTTGGTTTTGTGTGTATGGTAGCGAGTTTTCTCTACTTTGTCAGTTTTAAGAAGCTTAAACCAAAGGCTAATTCTGCTAGTTAG", "aa": "MRGKNRNKCNSIIFLTVGLLLFIAALALGVLVLFNGYQVNVNANGVDLKPFEIVHFPFAVKTFLSVLTFVLAAFGFVCMVASFLYFVSFKKLKPKANSAS", "aa_hexdigest": "652f9e3e0df54c69fe055bbf982575e8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD643B", "uniref100_id": "UniRef100_P75191", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD643B", "UniRef:UniRef100_P75191" ] }, "ips": { "uniref100_id": "UniRef100_P75191", "uniref90_id": "UniRef90_P75191" }, "psc": { "uniref90_id": "UniRef90_P75191", "product": "Uncharacterized protein MPN_594", "uniref50_id": "UniRef50_P75191", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75191", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75191" ], "product": "Uncharacterized protein MPN_594" }, "genes": [], "id": "GIHJHMOALN_1273", "locus": "GCA009939745_01273" }, { "type": "cds", "sequence": "contig_1", "start": 719021, "stop": 719479, "strand": "-", "frame": 3, "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "db_xrefs": [ "COG:COG0698", "COG:G", "EC:5.3.1.6", "GO:0004751", "GO:0009052", "GO:0019316", "KEGG:K01808", "RefSeq:WP_010874952.1", "SO:0001217", "UniParc:UPI000013973D", "UniRef:UniRef100_P53527", "UniRef:UniRef50_P47636", "UniRef:UniRef90_P53527" ], "nt": "ATGCAAATGAACCATCCAATTTACATTGCTTCTGACCACACTGGTTTAGAGCTCAAAAGCTTAGTGATAAAACACTTAGAACAACAAAAGTTGCAAGTGATTGATTTAGGTCCTACAGAGCTAGATCCACTTGATGACTACCCGGACTATGCCTTTTTGTTAGCGCAAACAATGCAAGCTAATCCTAATAGTTTAGGCATCCTTATTTGTGGTACTGGTGTGGGTGTTTGTATGGCCGCTAACAAGGCTAAGGGTATCTTAGCTGCTTTAGTGGTAGACAGTAAAACAGCAGCATTGGCACGCCAACATGATGACGCCAATGTGCTGTGTTTGTCTAGCCGCTTTGTGGTTCCTGAAGAAAACATTAAAATAGTAGATGAATTTCTCCAGGCGCAGTTTGAGGGTGGGCGCCATAGTAAACGTGTCGGTAAGATCATTGCTTATGAGAGGGAAAAATAG", "aa": "MQMNHPIYIASDHTGLELKSLVIKHLEQQKLQVIDLGPTELDPLDDYPDYAFLLAQTMQANPNSLGILICGTGVGVCMAANKAKGILAALVVDSKTAALARQHDDANVLCLSSRFVVPEENIKIVDEFLQAQFEGGRHSKRVGKIIAYEREK", "aa_hexdigest": "6b3e3d8771c22daa47888aaef1965a1e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013973D", "ncbi_nrp_id": "WP_010874952.1", "uniref100_id": "UniRef100_P53527", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013973D", "RefSeq:WP_010874952.1", "UniRef:UniRef100_P53527" ] }, "ips": { "uniref100_id": "UniRef100_P53527", "uniref90_id": "UniRef90_P53527" }, "psc": { "uniref90_id": "UniRef90_P53527", "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "ec_ids": [ "5.3.1.6" ], "uniref50_id": "UniRef50_P47636", "cog_id": "COG0698", "cog_category": "G", "kegg_orthology_id": "K01808", "go_ids": [ "GO:0004751", "GO:0009052", "GO:0019316" ] }, "pscc": { "uniref50_id": "UniRef50_P47636", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47636" ], "product": "Probable ribose-5-phosphate isomerase B" }, "genes": [ "rpiB" ], "id": "GIHJHMOALN_1274", "locus": "GCA009939745_01274" }, { "type": "cds", "sequence": "contig_1", "start": 719479, "stop": 721188, "strand": "-", "frame": 1, "gene": null, "product": "putative MG397-like protein", "db_xrefs": [ "GO:0016020", "RefSeq:WP_014325626.1", "SO:0001217", "UniParc:UPI0001DD643D", "UniRef:UniRef100_A0AAV5N757", "UniRef:UniRef50_P47637", "UniRef:UniRef90_Q50333" ], "nt": "ATGGTTGTCATTGCTGCTTTATTGGGATCGTTAGCTGTCTTAGCTTTTCTGTTTTACTTGTGGTATTTAACGATTTTCATTATTGTGCATAAAAACCAGCAGTACTTGGAACAGACCAAGGCGCTGTACAACAAGTTAAAACTCAGTAATTTCAACTCGGTAATTGTGCCGTTTCAACTGTTGCGCAGTGAAAAGAAAGCGCTGGAACAACCAGTGAAATTGCTCAAAAGTTTTCAAGAGAGCTTTAACAGCGAAATCAATTCCGCTTTAGAACAGCTGTTAGTTTTTAGTGAACCAAAAGCGCTTTACAACTGCTTTTGGTTTAACCGTAATATTAAGTTGTTGCGCAAAAACTTACAAGGATTACATGACAAGCAACAGCGTTATGTTAAGCTGACTAAAAACGCAATTGCGTACTTTGACAGTAGTTATGATACCCTAGTATTCTACCGACAAGCCTTTTGTTTACTAGTTAGTTTTATTAATGACTTTTTGGTGCATAAGTACGATTCCTTGTTTTACCTCAACATTATTAACCGCATTTCCTTATTGTTTAAGGATGTGGAACTACACATCAAAAATAAGGATGTGAAACAGCAAAACCAAGCGTTGAATAAACTCCACAATAACTTAGCACAAACCATTATTACCGCATCAAGACAATACTTCTTTGATGTCAAAGTAGGCTATTTGAACTACCACTTCCAAATTTTGAGTCAAAAGGTAACGCAATTGCGCTCGATGAAAAACAAAGCAATTAACTCCCAAGAGGTAGCTAAGTTTCAGGAATTAATTACTAATATTGGTGCTACTTTGGCGGAATGTAGCCAAGCGTTGGGCAACATTAACTTAGCGTTGTGTGAACATCGCATTAACCAAGCTAAGGAACAGATTGATGTGTTATCTAATGCTGTTAGTGTTAAAGAAAAAGCGATTAGCTTAGTGGTATCTAATGTGGATAGCTTCTTAAAGTCAATTAACCACTACCAACAACAAAACGCCTTGTTACAGACTTTAATGAAGGAAATTGAGTTAATGTTTCAAAACAACTTGGATACCGTTAACATCATTAACCAGATTAACGAACACTCGCTGTTAATTGCACAAAAAACCCAACTACTCAACCAGGAAAATGCGCTGACCGAATTCATTGATTATGAGAAGTTGTTTCGCTTAATGAGTGAAGCGATGAAGTTATTGGATGGATTGAAAAATCTGTTAAACGAGCTGTTTGCGCTGTCAGCTAATAAGTTTGATGATTACCGCTTTTTTGTCTATACCTTAGATGACTTTCGTTTTAAGTTTTTCCAAATTGAAAACTTAATTGCGAATGAAGAGTTAATTATTTCCGAAAAGACTTTAAAGATTATTAGTCAAGCTAAGCACCAGTTTGATGATATTTTTACCCAAGCGAAAAATGACTATGAGGGTGCTTTTAGCTATTTAAACGAAAAGGTGAGGTTCTTCCAAAACCAACTAACCCATGTGATTGTTGATGTAGTAGGTTTAATGAACCTGAGATCAATGTGTAAACATACCTTTATCTTTGCCAATAAGTACCGCCAAGAAAGCCCCCAAATTAACGAAAGCTTGGAAACGTTGACCCAGTTTTACCAACAGCAGAATTATTCGGAAACTTTGAGTGGTTTAATTAGTTTGTTGGGTAAAATTAAATCATCTGCTAAACAACACCACCTTGATTTAAACTAA", "aa": "MVVIAALLGSLAVLAFLFYLWYLTIFIIVHKNQQYLEQTKALYNKLKLSNFNSVIVPFQLLRSEKKALEQPVKLLKSFQESFNSEINSALEQLLVFSEPKALYNCFWFNRNIKLLRKNLQGLHDKQQRYVKLTKNAIAYFDSSYDTLVFYRQAFCLLVSFINDFLVHKYDSLFYLNIINRISLLFKDVELHIKNKDVKQQNQALNKLHNNLAQTIITASRQYFFDVKVGYLNYHFQILSQKVTQLRSMKNKAINSQEVAKFQELITNIGATLAECSQALGNINLALCEHRINQAKEQIDVLSNAVSVKEKAISLVVSNVDSFLKSINHYQQQNALLQTLMKEIELMFQNNLDTVNIINQINEHSLLIAQKTQLLNQENALTEFIDYEKLFRLMSEAMKLLDGLKNLLNELFALSANKFDDYRFFVYTLDDFRFKFFQIENLIANEELIISEKTLKIISQAKHQFDDIFTQAKNDYEGAFSYLNEKVRFFQNQLTHVIVDVVGLMNLRSMCKHTFIFANKYRQESPQINESLETLTQFYQQQNYSETLSGLISLLGKIKSSAKQHHLDLN", "aa_hexdigest": "cd32e1f5e6c683bf536750be336d0065", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD643D", "ncbi_nrp_id": "WP_014325626.1", "uniref100_id": "UniRef100_A0AAV5N757", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD643D", "RefSeq:WP_014325626.1", "UniRef:UniRef100_A0AAV5N757" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N757", "uniref90_id": "UniRef90_Q50333" }, "psc": { "uniref90_id": "UniRef90_Q50333", "product": "Uncharacterized protein MG397 homolog", "uniref50_id": "UniRef50_P47637", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47637", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47637" ], "product": "Uncharacterized protein MG397" }, "genes": [], "id": "GIHJHMOALN_1275", "locus": "GCA009939745_01275" }, { "type": "cds", "sequence": "contig_1", "start": 721188, "stop": 721589, "strand": "-", "frame": 2, "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "db_xrefs": [ "COG:C", "COG:COG0355", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0045261", "GO:0046933", "RefSeq:WP_010874954.1", "SO:0001217", "UniParc:UPI00001264FB", "UniRef:UniRef100_Q50332", "UniRef:UniRef50_P47638", "UniRef:UniRef90_Q50332" ], "nt": "ATGCAACCATTGCGCTTTTTAGTCTTAAGTCCCAGTGGTATTAAACTCGATCAAGCCATTATTAGTGCGCAAGTCAAAACCACTAATGGTTATTTGGGTTTGAACTATAACCGTGCGCCCTTAATTGCGGCCGTACAATCGCACTTTTGCAAAATTCTGTTTGCCAATAACACGCGGCGCAATGCCATCATAGGAGCGGGGTTAGTGCTCATTAAGAAAACTGAGGCCAAGATCTTTACCGAGAACTTTGTCTTTGAGGATGAAATAGATCTGGAAGCGACTTTGAAGCGTAAGGCCGAGTTAGAGCGGCAAATGAACCACTCGAAGGACTTAAAGCTCAACATTAAGATTGAACAAAACTTAATGTTTGAACTGTTAAAGCTTTCCAATAAGCAACGCTAA", "aa": "MQPLRFLVLSPSGIKLDQAIISAQVKTTNGYLGLNYNRAPLIAAVQSHFCKILFANNTRRNAIIGAGLVLIKKTEAKIFTENFVFEDEIDLEATLKRKAELERQMNHSKDLKLNIKIEQNLMFELLKLSNKQR", "aa_hexdigest": "cf26a0ac06b3935c1b93e9248ddb46c2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001264FB", "ncbi_nrp_id": "WP_010874954.1", "uniref100_id": "UniRef100_Q50332", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001264FB", "RefSeq:WP_010874954.1", "UniRef:UniRef100_Q50332" ] }, "ips": { "uniref100_id": "UniRef100_Q50332", "uniref90_id": "UniRef90_Q50332" }, "psc": { "uniref90_id": "UniRef90_Q50332", "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47638", "cog_id": "COG0355", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045261", "GO:0005524", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47638", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47638" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "GIHJHMOALN_1276", "locus": "GCA009939745_01276" }, { "type": "cds", "sequence": "contig_1", "start": 721592, "stop": 723019, "strand": "-", "frame": 3, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961", "RefSeq:WP_014325627.1", "SO:0001217", "UniParc:UPI0002386D1F", "UniRef:UniRef100_A0AB33HPE1", "UniRef:UniRef50_P50002", "UniRef:UniRef90_Q50331" ], "nt": "ATGAAAAAGGAAAACATTACATACGGTAAGGTCCACCAAGTGATTGGCCCGGTAGTTGATGTCATCTTTACGGAAAGTAGTCAGTTACCCCGCATTTACGATTGTTTGAGTGTTAAGTTAGCTGGGGAAGAACTGTTTTTGGAAGCCGCGCAGCTAATAGGCGATGACATTGTGCGCTGCATTGCTTTGGGTCCAACTGAAGGGTTAGCACGCAACGAAAAGGTGACTAACTATAACCACCCGATTGAAGTCCCAGTTGGCAAAAACGTCTTGGGCCGGATGTTTAATGTTTTGGGTAAACCCATTGACGGGAAGGAGGAGTTACCCAAAAAACCACAACTCCCGATTCACCGCAAACCACCTTCGTTTGATGACCAGTCCAATACGCTAGAAATCTTTGAAACAGGGATTAAGGTGATTGACTTGCTCACTCCCTATGCCCGTGGTGGTAAGATTGGTTTATTTGGTGGTGCTGGTGTTGGTAAAACGGTTTTAGTGCAAGAGTTAATCCACAACATTGCTAAGGAACACTCTGGTTTAAGTGTGTTTGCTGGTGTGGGGGAACGCACCCGGGAAGGTAATGATCTCCACTATGAAATGATCCAGGGTGGGGTGATTGACAAAACTGCTTTAGTGTTTGGTCAGATGAACGAACCCCCAGGAGCACGGATGCGGGTCGCTTTAACGGCGCTTACTATGGCCGAATACTTCCGTGACCATGACAACCAGGATGTCTTGCTCTTCATTGACAACATCTTCCGTTTTACCCAAGCTGGTAGTGAGGTATCAGCACTGTTAGGTCGGATGCCATCAGCCGTGGGTTACCAACCAACGTTGGCGACTGAAATGGGGCAATTACAAGAGCGGATTGCTTCCACTAAAACCGGTTCGATTACCTCGGTGCAAGCGATCTATGTGCCAGCTGATGACTTGACTGACCCCGCTCCGGCTACCACCTTTACCCACCTGGATGCTAAAACGGTGTTAGACCGGAACATAGCAGCGTTGGGAATTTTTCCAGCGATCAATCCCTTAGAGTCTACCAGTCGCTTATTGGATCCTAATATTGTCGGGATTAACCACTATAAGGTAGCGTTAGGGGTGCAAAACATCTTACAGCGCTTTGCGGAACTACAAGACATTATTGCCATCTTGGGAATTGATGAGTTAGCGGATGAGGACAAGATCATTGTGGAACGGGCACGCCGGATCCGCAACTTCCTCTCCCAACCGTTCTTTGTAGCGGAAAAGTTCTCGGGGATTGCTGGGAAGTATGTCCCTTTAAGTGACACGATCCAATCGTTTAAGGAAATCTTGGACGGTAAGCATGATGATCTCCCCGAACAGGCCTTCTTCTTTGTTGGTACCATTCAGGAAGCAGTGGAGAAAGCGAAGCGGCTTAAAAAAGCTACTGTTGAGGAGAAATAA", "aa": "MKKENITYGKVHQVIGPVVDVIFTESSQLPRIYDCLSVKLAGEELFLEAAQLIGDDIVRCIALGPTEGLARNEKVTNYNHPIEVPVGKNVLGRMFNVLGKPIDGKEELPKKPQLPIHRKPPSFDDQSNTLEIFETGIKVIDLLTPYARGGKIGLFGGAGVGKTVLVQELIHNIAKEHSGLSVFAGVGERTREGNDLHYEMIQGGVIDKTALVFGQMNEPPGARMRVALTALTMAEYFRDHDNQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGQLQERIASTKTGSITSVQAIYVPADDLTDPAPATTFTHLDAKTVLDRNIAALGIFPAINPLESTSRLLDPNIVGINHYKVALGVQNILQRFAELQDIIAILGIDELADEDKIIVERARRIRNFLSQPFFVAEKFSGIAGKYVPLSDTIQSFKEILDGKHDDLPEQAFFFVGTIQEAVEKAKRLKKATVEEK", "aa_hexdigest": "bbff0f3afd75ce65267b83c45176cffc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386D1F", "ncbi_nrp_id": "WP_014325627.1", "uniref100_id": "UniRef100_A0AB33HPE1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386D1F", "RefSeq:WP_014325627.1", "UniRef:UniRef100_A0AB33HPE1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB33HPE1", "uniref90_id": "UniRef90_Q50331" }, "psc": { "uniref90_id": "UniRef90_Q50331", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P50002", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P50002", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P50002" ], "product": "ATP synthase subunit beta, sodium ion specific" }, "genes": [ "atpD" ], "id": "GIHJHMOALN_1277", "locus": "GCA009939745_01277" }, { "type": "cds", "sequence": "contig_1", "start": 723019, "stop": 723453, "strand": "-", "frame": 1, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_Q50330" ], "nt": "TTGAGCTTTGATTACTGTCAACAGGTGTCCGACCAAATTATGGAGCAGTTTGATTTACACCAATTAGACCGTATTTGCATCATTTACACCCAGTTTAAAAACCCCTTAATTCAACACGCCAACAGCTTCCAGGTTTTTCCCTTTGATGTAGCGATGTTTAAGGCCTTCAACCCGGTCAAAATGGAACAAAAGCTCGACTTTGAACCCGATGAGGAGACAATTATTAAGCTCATTAGTCCCCAGTTCTTTGACATTGCGTTATACGGTGGGTTGGTAGAGACAAAGTTGTGTGAGTCAGCATCAAGACAAAACGCCATGGATGCCGCTGCTAAGAATGCCAAGGATCTGTATGAAAAGTACAGTATTCAATTTAATAAGTTAAGACAAAACTCTATTACCCAAGAAATTATTGAAATCATAGGAGGTATTAGTTAA", "aa": "MSFDYCQQVSDQIMEQFDLHQLDRICIIYTQFKNPLIQHANSFQVFPFDVAMFKAFNPVKMEQKLDFEPDEETIIKLISPQFFDIALYGGLVETKLCESASRQNAMDAAAKNAKDLYEKYSIQFNKLRQNSITQEIIEIIGGIS", "aa_hexdigest": "118938d06364c706d2bc1fb92b85c8bf", "start_type": "TTG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "3-prime", "pseudogene": { "start": 723019, "stop": 723753, "observations": { "mutation": [ 723513 ], "stop": [ 723513 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50330", "query_cov": 1.0, "subject_cov": 0.5161290322580645, "identity": 0.993, "score": 286.0, "evalue": 9.69e-96, "gene_start": 1, "gene_end": 144, "reference_start": 136, "reference_end": 279, "reference_sequence": "MAFIQEIKRKMTTVQSTIKITNAMKMVSRAKFVRFKKQFKEVNYFFNEFYKAVGQVVLSLKKMPKPLENPKTLWVMMSSSLGLCGQHNTNMNKLLQTKFQPGDKIFFLGRKNQSFWNKGDTDNPTVGYVDIQDRDLSFDYCQQVSDQIMEQFDLHQLDRICIIYTQFKNPLIQHANSFQVFPFDVAMFKAFNPVKMEQKLDFEPDEETIIKLISPQFFDIALYGGLVETKLCESASRQNAMDAAAKNAKDLYEKYSIQFNKLRQNSITQEIIEIIGGIS" }, "paralog": false, "identity": 0.9959016393442623, "score": 492.0, "evalue": 2.64e-179, "description": "Internal stop codon at 723513. Nonsense mutation around 723513.", "psc": { "uniref90_id": "UniRef90_Q50330", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50330", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "GIHJHMOALN_1278", "locus": "GCA009939745_01278" }, { "type": "cds", "sequence": "contig_1", "start": 723511, "stop": 723858, "strand": "-", "frame": 1, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_Q50330" ], "nt": "ATGGCCTTTATCCAGGAAATTAAGCGCAAAATGACCACGGTGCAGTCCACCATTAAGATTACTAACGCAATGAAAATGGTGTCCCGTGCTAAGTTTGTCCGCTTTAAAAAACAGTTTAAGGAAGTTAACTACTTCTTCAATGAGTTTTATAAGGCCGTTGGGCAAGTAGTTCTCAGCTTAAAGAAGATGCCCAAGCCCCTGGAAAATCCCAAAACCCTGTGGGTGATGATGAGTTCATCGTTGGGTCTTTGTGGACAGCACAACACTAACATGAACAAGTTGTTGCAAACGAAGTTCCAACCCGGTGACAAAATCTTCTTTTTGGGGCGCAAAAACCAGTCTTTTTGA", "aa": "MAFIQEIKRKMTTVQSTIKITNAMKMVSRAKFVRFKKQFKEVNYFFNEFYKAVGQVVLSLKKMPKPLENPKTLWVMMSSSLGLCGQHNTNMNKLLQTKFQPGDKIFFLGRKNQSF", "aa_hexdigest": "d84c18f22f3a98013e8fd9bae2cc8332", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "5-prime", "pseudogene": { "start": 723208, "stop": 723858, "observations": { "mutation": [ 723513 ], "stop": [ 723513 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50330", "query_cov": 1.0, "subject_cov": 0.4121863799283154, "identity": 1.0, "score": 228.0, "evalue": 1.27e-73, "gene_start": 1, "gene_end": 115, "reference_start": 1, "reference_end": 115, "reference_sequence": "MAFIQEIKRKMTTVQSTIKITNAMKMVSRAKFVRFKKQFKEVNYFFNEFYKAVGQVVLSLKKMPKPLENPKTLWVMMSSSLGLCGQHNTNMNKLLQTKFQPGDKIFFLGRKNQSFWNKGDTDNPTVGYVDIQDRDLSFDYCQQVSDQIMEQFDLHQLDRICIIYTQFKNPLIQHANSFQVFPFDVAMFKAFNPVKMEQKLDFEPDEETIIKLISPQFFDIALYGGLVETKLCESASRQNAMDAAAKNAKDLYEKYSIQFNKLRQNSITQEIIEIIGGIS" }, "paralog": false, "identity": 0.9953703703703703, "score": 436.0, "evalue": 1.44e-157, "description": "Internal stop codon at 723513. Nonsense mutation around 723513.", "psc": { "uniref90_id": "UniRef90_Q50330", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50330", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "GIHJHMOALN_1279", "locus": "GCA009939745_01279" }, { "type": "cds", "sequence": "contig_1", "start": 723858, "stop": 725414, "strand": "-", "frame": 2, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "db_xrefs": [ "COG:C", "COG:COG0056", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961", "RefSeq:WP_014325628.1", "SO:0001217", "UniParc:UPI0001DD644D", "UniRef:UniRef100_A0AB38W5W8", "UniRef:UniRef50_P33252", "UniRef:UniRef90_Q50329" ], "nt": "ATGGCAGACAAATTAAATGAATATGTAGCACTCATTAAAAATGAGATTAAGAAGTATTCCAAACAGATCTTTAACAGTGAAATTGGCAAGGTTATTAGTGTCGCTGATGGCATTGCTAAGGTCAGTGGTTTGGAAAACGCACTCTTAAACGAGTTAATTGAGTTTGAAAACAACGTGCAGGGGATTGCGCTTAACCTAGAGCAAAACACGGTTGGGGTGGCGTTGTTTGGTGATTACTCTAAAATCCGTGAAGGTAGTACGGCTAAACGGACCCACAACGTGATGCAAACTCCCGTGGGTGATGTAATGTTGGGCCGGATTGTCAATGCCCTGGGAGAACCGGTGGATGGTCGTGGTCCGATTAAGGCTGAAGAGTTTGACCAAGTGGAGAAGATTGCCCCTGGGGTAATGACCCGCAAAACCGTTAACCAACCACTGGAAACTGGAATCTTGACGATTGATGCGTTGTTTCCGATTGGTAAGGGACAACGGGAGTTAATTGTGGGGGACCGCCAAACTGGTAAGACCTCAATTGCAATTGACACCATTATTAACCAACGTGGTAAGGACGTGTACTGTGTCTATGTAGCGATGGGACAAAAGAATTCCTCGGTAGCGCAAATTGTCCACCAGTTAGAGGTAACTGATTCGATGAAGTACACCACGGTGGTGTGTGCTACAGCCAGTAATCCCGCTTCGATGATCTACCTCACGCCCTTTACTGGCATTACCATTGCTGAGTACTGGCTCAAACAGGGTAAGGATGTTCTAATTGTGTTTGATGATTTGTCCAAGCACGCGATTGCTTACCGTACCTTATCCTTACTGTTACGCCGACCACCAGGACGGGAAGCCTTTCCTGGGGATGTGTTCTACTTGCACTCGCGTTTACTGGAACGTGCTTGCCGTTTAAACGAGGAGCATGGTGGTGGCTCGATTACCGCTTTACCTATTATTGAAACGCAAGCTGGGGACATTTCCGCTTACATTCCGACCAACGTCATTTCGATTACCGATGGTCAACTGTTCATGGTGTCAAACCTGTTTAACTCCGGTCAACGCCCTGCCATCCACGTGGGGTTATCGGTGTCGCGGGTTGGTAGTGCAGCGCAAATTAAAGCAATTAAACAACAAACCGGGAGCTTAAAGTTAGAGCTAGCGCAGTACAGTGAATTAGACAGCTTTAGTCAGTTTGGTAGTGACTTGGACGAAAATACCAAGCAAATCTTGGAACGCGGTAAGCGGGTAATGGAGATGATTAAACAACCCAACGGTAAACCTTACTCACAAACCCATGAAGCGTTATTCTTGTTTGCGATTGCCAAGTCCTTCATTAAGTTTATTCCACTGGATTACATTGCTAAATTTAAGCAACGCATTATGGAGGAATTTGACAAGGAACACCCGATTTACAAGGAAATAGCGACCCAAAAGAGCTTTAGTGAAGCGTTGGAAGCACAAACTAACACTGCTTTTAAGGCACTAGTAAAACGCTTTGTTAGTGCTTTACCAGATTACGACATCACTAAATACGGAACGATGGAGGAGTTGGAATAA", "aa": "MADKLNEYVALIKNEIKKYSKQIFNSEIGKVISVADGIAKVSGLENALLNELIEFENNVQGIALNLEQNTVGVALFGDYSKIREGSTAKRTHNVMQTPVGDVMLGRIVNALGEPVDGRGPIKAEEFDQVEKIAPGVMTRKTVNQPLETGILTIDALFPIGKGQRELIVGDRQTGKTSIAIDTIINQRGKDVYCVYVAMGQKNSSVAQIVHQLEVTDSMKYTTVVCATASNPASMIYLTPFTGITIAEYWLKQGKDVLIVFDDLSKHAIAYRTLSLLLRRPPGREAFPGDVFYLHSRLLERACRLNEEHGGGSITALPIIETQAGDISAYIPTNVISITDGQLFMVSNLFNSGQRPAIHVGLSVSRVGSAAQIKAIKQQTGSLKLELAQYSELDSFSQFGSDLDENTKQILERGKRVMEMIKQPNGKPYSQTHEALFLFAIAKSFIKFIPLDYIAKFKQRIMEEFDKEHPIYKEIATQKSFSEALEAQTNTAFKALVKRFVSALPDYDITKYGTMEELE", "aa_hexdigest": "aa7c409612fcd9c1f0b44434d938fe62", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD644D", "ncbi_nrp_id": "WP_014325628.1", "uniref100_id": "UniRef100_A0AB38W5W8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD644D", "RefSeq:WP_014325628.1", "UniRef:UniRef100_A0AB38W5W8" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W5W8", "uniref90_id": "UniRef90_Q50329" }, "psc": { "uniref90_id": "UniRef90_Q50329", "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P33252", "cog_id": "COG0056", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0043531", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P33252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P33252" ], "product": "ATP synthase subunit alpha" }, "genes": [ "atpA" ], "id": "GIHJHMOALN_1280", "locus": "GCA009939745_01280" }, { "type": "cds", "sequence": "contig_1", "start": 725417, "stop": 725953, "strand": "-", "frame": 3, "gene": "atpH", "product": "ATP synthase F1 subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_014325629.1", "SO:0001217", "UniParc:UPI0001DD644E", "UniRef:UniRef100_A0AAV5NA96", "UniRef:UniRef50_P47642", "UniRef:UniRef90_Q50328" ], "nt": "ATGATTAACGCGCAGGCTTTTGGAATGGCACTCTTCCAACTTAGTGAAGAGGAAAAGAAGGTTAAGAAGGTCTATGACCAGAGCCATGACTTTTTAAAACTAGCGCGTAACTTTAAAGATGGATCACTAGCTGCTCTACTCAATACTTATACTTTAACCAAAAAGGAAAAGCTTAAGCTAATAGATAAGCTGTTTAAAAACCACTTTTGTGACCTCTTTGTTGACTTTTTAAAAACGATTGTGCTCAAGGGCTACTTTAACCTAGTCGAGCAAGCCTTAAAGTACTTCTTTGATTGTGTTGAGAATGAAAAACACGTCCAGTTCATTAGGATCATTACCGCTTTTGAACTGAGTGCGCCCCAACTCAAGCGAATCGTAGCGGCAATGGAAAAGAAGCTCAATTCCAAGATTGTTTACAAAACCGAAATTGACAAGAGCTTGATTTCGGGTATCCGAATTGAATCGAGCGCCCAGTTATTTGAAAAGAATATCCGTGATCAACTATCACGTTTAATGGAACAGTTTAAAGGGAATTAA", "aa": "MINAQAFGMALFQLSEEEKKVKKVYDQSHDFLKLARNFKDGSLAALLNTYTLTKKEKLKLIDKLFKNHFCDLFVDFLKTIVLKGYFNLVEQALKYFFDCVENEKHVQFIRIITAFELSAPQLKRIVAAMEKKLNSKIVYKTEIDKSLISGIRIESSAQLFEKNIRDQLSRLMEQFKGN", "aa_hexdigest": "13f5afe57158869d964d14340f523d4d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD644E", "ncbi_nrp_id": "WP_014325629.1", "uniref100_id": "UniRef100_A0AAV5NA96", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD644E", "RefSeq:WP_014325629.1", "UniRef:UniRef100_A0AAV5NA96" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NA96", "uniref90_id": "UniRef90_Q50328" }, "psc": { "uniref90_id": "UniRef90_Q50328", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47642", "cog_id": "COG0712", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47642", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47642" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "GIHJHMOALN_1281", "locus": "GCA009939745_01281" }, { "type": "cds", "sequence": "contig_1", "start": 725946, "stop": 726320, "strand": "-", "frame": 2, "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "db_xrefs": [ "COG:C", "COG:COG0711", "EC:7.1.2.2", "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961", "SO:0001217", "UniRef:UniRef50_Q50327", "UniRef:UniRef90_Q50327" ], "nt": "TTGGAAGAGCAAGTCAACCAGGCCAACAGCTTAGAACAACAAGCTCAAGCATTACTCCAACAAGCCAACCAACGCCATGAAAACTCACTGGTAGTTGCTAAGGAAATTGTAGACCAGGCCAACTATGAGGCGTTGCAACTTAAAAGTGAAATCGAAAAGAAGGCTAACCGGCAAGCTAACTTAATGATCTTCCAAGCGCGTCAGGAAATTGAAAAGGAAAAGCGCTTGATCCAAGAACAATCCTTAAAGGAAAGTGTCGAGCTAGCCATGTTAGCTGCTAAGGAATTGATTATCAAAAAGGTGGATGTCAAAGCCGACAAGGCCTTTATTGAAGAGTTTATCCGCGAACTAGAAGCAGAGGACGACCATGATTAA", "aa": "MEEQVNQANSLEQQAQALLQQANQRHENSLVVAKEIVDQANYEALQLKSEIEKKANRQANLMIFQARQEIEKEKRLIQEQSLKESVELAMLAAKELIIKKVDVKADKAFIEEFIRELEAEDDHD", "aa_hexdigest": "0a202e2ebfc50ed763cad45145aeba80", "start_type": "TTG", "rbs_motif": null, "genes": [ "atpF" ], "truncated": "3-prime", "pseudogene": { "start": 725946, "stop": 726569, "observations": { "mutation": [ 726362 ], "stop": [ 726362 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50327", "query_cov": 1.0, "subject_cov": 0.5990338164251208, "identity": 0.992, "score": 210.0, "evalue": 3.69e-67, "gene_start": 1, "gene_end": 124, "reference_start": 84, "reference_end": 207, "reference_sequence": "MKLRATFVFKTTLVALSFALFALFLVSCTENVKEIKSESVINELFPNLWVFLAHLLAFVILLFLLLFLFWKPTQKFLNQRKALLEEQVNQANSLEQQAQALLQQANQRHENSLVVAKEIVDQANYEALQLKSEIEKKANRQANLMIFQARQEIEKEKRLIQEQSLKESVELAMLAAKELIIKKVDVKADKAFIEEFIRELEAEDDHD" }, "paralog": false, "identity": 0.9951690821256038, "score": 386.0, "evalue": 4.64e-139, "description": "Internal stop codon at 726362. Nonsense mutation around 726362.", "psc": { "uniref90_id": "UniRef90_Q50327", "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q50327", "cog_id": "COG0711", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50327", "EC:7.1.2.2", "UniRef:UniRef50_Q50327", "COG:COG0711", "COG:C", "GO:0005886", "GO:0015078", "GO:0015986", "GO:0045263", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_Q50327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50327" ], "product": "ATP synthase subunit b" } }, "id": "GIHJHMOALN_1282", "locus": "GCA009939745_01282" }, { "type": "cds", "sequence": "contig_1", "start": 726360, "stop": 726569, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTACGAGCAACTTTTGTATTTAAAACGACCTTAGTTGCGCTTAGTTTTGCCCTGTTTGCCTTGTTTTTAGTTTCCTGTACCGAAAACGTTAAGGAAATTAAAAGTGAATCAGTCATCAACGAACTTTTTCCGAACCTCTGGGTCTTTTTAGCCCACTTGTTGGCCTTTGTGATCCTGTTGTTCTTGTTGTTGTTCCTCTTTTGA", "aa": "MKLRATFVFKTTLVALSFALFALFLVSCTENVKEIKSESVINELFPNLWVFLAHLLAFVILLFLLLFLF", "aa_hexdigest": "5878f6f07991407453cf537e44b74ec1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7955.630299999993, "isoelectric_point": 7.940844917297363 }, "id": "GIHJHMOALN_1283", "locus": "GCA009939745_01283" }, { "type": "cds", "sequence": "contig_1", "start": 726572, "stop": 726889, "strand": "-", "frame": 3, "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "db_xrefs": [ "COG:C", "COG:COG0636", "EC:7.1.2.2", "GO:0005886", "GO:0008289", "GO:0045263", "GO:0046933", "RefSeq:WP_010874960.1", "SO:0001217", "UniParc:UPI00001265D2", "UniRef:UniRef100_Q59550", "UniRef:UniRef50_P47644", "UniRef:UniRef90_Q59550" ], "nt": "ATGGAACACGTTAATGAAATTCTCGCCACAGTTGGCCGCATCTTACACGAAACAACAACAGCTAATACCAATGTTGCCAATAAAAGTACCGAACGTTTAGGGGCTTACATTGGGGCTGGGATTACCATGGTCGGTGGTGCGACCGTGGGTCTTGGTCAAGGTTACATCTTTGGGAAAGCGGTTGAAGCCGTAGCGCGGAACCCCGAAGTGGAAAAGCAGGTCTTTAAATTAATCTTTATTGGCTCTGCTATTAGTGAATCGAGTTCCATCTATTCCTTGTTAATAGCCTTCATTCTTATCTTTGTATCGGGCGCTTAA", "aa": "MEHVNEILATVGRILHETTTANTNVANKSTERLGAYIGAGITMVGGATVGLGQGYIFGKAVEAVARNPEVEKQVFKLIFIGSAISESSSIYSLLIAFILIFVSGA", "aa_hexdigest": "db463fb393f3e86270e9f374118cb4dc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001265D2", "ncbi_nrp_id": "WP_010874960.1", "uniref100_id": "UniRef100_Q59550", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001265D2", "RefSeq:WP_010874960.1", "UniRef:UniRef100_Q59550" ] }, "ips": { "uniref100_id": "UniRef100_Q59550", "uniref90_id": "UniRef90_Q59550" }, "psc": { "uniref90_id": "UniRef90_Q59550", "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47644", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045263", "GO:0008289", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47644" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "GIHJHMOALN_1284", "locus": "GCA009939745_01284" }, { "type": "cds", "sequence": "contig_1", "start": 726892, "stop": 727242, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00119.25" ], "nt": "TTGAGCTTCTTGGGTGAGTTTGCGCCACTGTTCTCGATTTCGCTTCGTTTGTGGGGCAATATCCTTGGGGGAACCTTGATTTTAGCGCTCTTTTATAACTTCTGGTTCTTTGCTTTTTCGACTTTAAGCAATAAGCCACTAGCGCTTAGTTTAGGTGCCATTTTTGCAGGAATTCTCACCCCAGCCCTACATGTATATTTTGATGTAGTAGTGGGTACACTGCAGGGATATGTCTTTGTGATGTTGACTTATAATTATTGGGCAAAAATGCGTAACATTGGCTTGGAAGAAAGTCAAGAAGCAGCGCAGCGTTTGCAAAATTTGGAGGTAGCAAAAGAAATAATTAATTAG", "aa": "MSFLGEFAPLFSISLRLWGNILGGTLILALFYNFWFFAFSTLSNKPLALSLGAIFAGILTPALHVYFDVVVGTLQGYVFVMLTYNYWAKMRNIGLEESQEAAQRLQNLEVAKEIIN", "aa_hexdigest": "965d7137397394ff0a697528c60a6335", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00119.25", "name": "ATP-synt_A", "length": 90, "aa_cov": 0.7413793103448276, "hmm_cov": 0.9, "evalue": 5.74249390389205e-19, "score": 64.29066467285156, "start": 2, "stop": 87 } ], "seq_stats": { "molecular_weight": 13047.127499999991, "isoelectric_point": 5.668689155578614 }, "id": "GIHJHMOALN_1285", "locus": "GCA009939745_01285" }, { "type": "cds", "sequence": "contig_1", "start": 727259, "stop": 727471, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGTACTTCTTGCCCTTAATCGTAATGTTGAAGGTAAAGCTTTTAAAGAAGGATCACCGTTGGTACCTAATCCCCATAATGACAATTCCCAAAAAGGTGGCTAGCCCTAAAGAAAAGGTAAAGGTCAGTGATGAAGTTGGTGGGGAAATGCCACCTAATAATCCAATCAAGTTACTGGAAACCAGGTACAACAGCAACATTAAAAAGTAA", "aa": "MEYFLPLIVMLKVKLLKKDHRWYLIPIMTIPKKVASPKEKVKVSDEVGGEMPPNNPIKLLETRYNSNIKK", "aa_hexdigest": "9e2672b52be875872eced49919f45324", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8192.855099999997, "isoelectric_point": 9.822548103332519 }, "id": "GIHJHMOALN_1286", "locus": "GCA009939745_01286" }, { "type": "cds", "sequence": "contig_1", "start": 728254, "stop": 729588, "strand": "-", "frame": 1, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "COG:COG0148", "COG:G", "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0005829", "GO:0006096", "GO:0009986", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_P75189", "UniRef:UniRef90_P75189" ], "nt": "ATGAGTGCACAAACTGGAACCGATCTTTTCAAGATTGCTGATCTTTTTGCTTACCAAGTTTTTGATTCACGGGGTTTTCCCACTGTTGCTTGTGTTGTGAAGTTAGCTAGTGGTCATACTGGAGAAGCGATGGTTCCATCTGGGGCTTCTACTGGGGAAAAAGAAGCGATTGAACTACGCGATGGTGATCCCAAAGCTTACTTTGGCAAAGGGGTGAGCCAAGCTGTGCAAAACGTTAACCAAACTATTGCCCCTAAACTAATTGGTTTAAATGCGACTGATCAAGCAGCCATTGATGCTTTAATGATCCAACTGGATGGTACCCCCAATAAGGCTAAGTTAGGTGCTAATGCCATTTTGGCAGTTTCTCTAGCTGTGGCTAAAGCAGCTGCTAGCGCCCAAAAGACTTCCTTGTTTAAGTACCTTGCTAACCAGGTCATGGGTCTAAATAAAACCGAGTTTATCCTCACCGTACCAATGCTAAACGTCATTAACGGTGGAGCACATGCTGACAATAACATTGACTTCCAGGAGTTCATGATTATGCCATTGGGTGCTAACTCGATGCACCAAGCATTGAAGATGGCGAGTGAAACCTTCCATGCTTTACAAAAGCTGTTAAAGCAACGGGGTTTAAACACCAATAAGGGTGATGAAGGTGGTTTTGCGCCTAACTTAAAGTTAGCGGAAGAAGCGCTTGATTTAATGGTCGAAGCAATTAAGGCAGCTGGGTACCAACCAGGTAGTGACATTGCGATTGCACTGGATGTAGCAGCGAGTGAGTTTTACGATGACACCACCAAGCGCTATGTCTTCAAAAAAGGAATTAAGGCCAAAATTCTTGACGAGAAGGAATGGAGTTTAACTACTGCCCAAATGATCGCTTACCTCAAGAAGCTTACTGAGCAGTACCCTATTATTTCCATAGAAGATGGTCTTAGCGAACACGACTGGGAAGGTATGGAAACATTAACTAAAACACTGGGTCAACACATTCAAATAGTTGGTGATGATCTGTACTGCACCAATCCAGCAATTGCTGAAAAAGGTGTTGCTCACAAAGCTACCAACTCGATCTTAATTAAACTCAACCAAATTGGTACCCTTACAGAAACAATTAAAGCGATTAACATTGCCAAGGACGCTAACTGGAGTCAGGTAATTTCCCACCGTAGTGGGGAAACCGAAGACACGACAATTGCTGATCTAGCAGTAGCGGCTTGCACTGGTCAAATTAAAACTGGTTCGATGTCCCGCTCCGAACGAATTGCCAAATACAATCGCTTATTGCAAATTGAACTGGAATTGGGCAATAATGCTAAGTACTTAGGTTGA", "aa": "MSAQTGTDLFKIADLFAYQVFDSRGFPTVACVVKLASGHTGEAMVPSGASTGEKEAIELRDGDPKAYFGKGVSQAVQNVNQTIAPKLIGLNATDQAAIDALMIQLDGTPNKAKLGANAILAVSLAVAKAAASAQKTSLFKYLANQVMGLNKTEFILTVPMLNVINGGAHADNNIDFQEFMIMPLGANSMHQALKMASETFHALQKLLKQRGLNTNKGDEGGFAPNLKLAEEALDLMVEAIKAAGYQPGSDIAIALDVAASEFYDDTTKRYVFKKGIKAKILDEKEWSLTTAQMIAYLKKLTEQYPIISIEDGLSEHDWEGMETLTKTLGQHIQIVGDDLYCTNPAIAEKGVAHKATNSILIKLNQIGTLTETIKAINIAKDANWSQVISHRSGETEDTTIADLAVAACTGQIKTGSMSRSERIAKYNRLLQIELELGNNAKYLG", "aa_hexdigest": "bafd65c3ff0d85920093de9bf30d0326", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75189", "query_cov": 1.0, "subject_cov": 0.9568965517241379, "identity": 1.0, "score": 859.0, "evalue": 2.48e-314, "valid": true, "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_P75189", "cog_id": "COG0148", "cog_category": "G", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0005576", "GO:0005829", "GO:0006096", "GO:0009986" ] }, "pscc": { "uniref50_id": "UniRef50_P75189", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75189" ], "product": "Enolase" }, "genes": [ "eno" ], "id": "GIHJHMOALN_1287", "locus": "GCA009939745_01287" }, { "type": "cds", "sequence": "contig_1", "start": 729643, "stop": 730116, "strand": "+", "frame": 1, "gene": "msrA", "product": "Peptide methionine sulfoxide reductase MsrA", "db_xrefs": [ "COG:COG0225", "COG:O", "EC:1.8.4.11", "GO:0005737", "GO:0008113", "GO:0034599", "GO:0036211", "GO:0036456", "RefSeq:WP_010874964.1", "SO:0001217", "UniParc:UPI000012F657", "UniRef:UniRef100_P75188", "UniRef:UniRef50_P75188", "UniRef:UniRef90_P75188" ], "nt": "ATGAAACAAATCTATTTTGGTGGTGGCTGTTTTTGGGGTACCCAAAAGTATTTTGATCTGATTAAGGGTGTCCAAAAGACCAGTGTGGGCTACCTCAATTCCAACATGAAAAATCCCACTTACGAACAAGTATGTAGTGGGCAGACCAACGCAGTGGAAGCAGTGTTCGTTGAATATGATGAAAACAAGGTAAGCTTAAACGAACTTATTGATGCTTTCTTTAAGGTCATTGATCCCACCATTAGAAACCGTCAGGGTAATGATATTGGTACCCAGTACCGTACCGGAGTTTATTGGGTAGATCCCCAAGACGAGCAGTTAATTACGCAAAAGTTTAGGGAACTACAGGCCAATTATCCAAAACCAATTGTGACGGAAAACCGTGCAATGGAAAACTATTTTTTAGCAGAAGAGTACCACCAGGATTACTTAAAGAAAAATCCTCATGGTTACTGTCACATTAAGTTTGACTAG", "aa": "MKQIYFGGGCFWGTQKYFDLIKGVQKTSVGYLNSNMKNPTYEQVCSGQTNAVEAVFVEYDENKVSLNELIDAFFKVIDPTIRNRQGNDIGTQYRTGVYWVDPQDEQLITQKFRELQANYPKPIVTENRAMENYFLAEEYHQDYLKKNPHGYCHIKFD", "aa_hexdigest": "a6f32d55a9980d1a2810341e441f7a7f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012F657", "ncbi_nrp_id": "WP_010874964.1", "uniref100_id": "UniRef100_P75188", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012F657", "RefSeq:WP_010874964.1", "UniRef:UniRef100_P75188" ] }, "ips": { "uniref100_id": "UniRef100_P75188", "uniref90_id": "UniRef90_P75188" }, "psc": { "uniref90_id": "UniRef90_P75188", "gene": "msrA", "product": "Peptide methionine sulfoxide reductase MsrA", "ec_ids": [ "1.8.4.11" ], "uniref50_id": "UniRef50_P75188", "cog_id": "COG0225", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0036456", "GO:0008113", "GO:0034599", "GO:0036211" ] }, "pscc": { "uniref50_id": "UniRef50_P75188", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75188" ], "product": "Peptide methionine sulfoxide reductase MsrA" }, "genes": [ "msrA" ], "id": "GIHJHMOALN_1288", "locus": "GCA009939745_01288" }, { "type": "cds", "sequence": "contig_1", "start": 730113, "stop": 730514, "strand": "-", "frame": 2, "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "db_xrefs": [ "COG:COG0704", "COG:P", "GO:0006817", "GO:0030643", "GO:0045936", "SO:0001217", "UniRef:UniRef50_P47649", "UniRef:UniRef90_P75187" ], "nt": "ATGTGTAGTCGTGATTTGGAACGCATTTGTGATTATGCCAATAACCTCACTAAGTTTGTCAAGCACTACCAGCACTTGGATGTCAGCATCTTTAGTAAGTTAGTTAATTTGCACAAAAGTGTGTTGAACAACTTAAAGCAAACGTTTGCCAGTTTACAAGATAAGGAAAAACCGCTTACTATTCAGTTTGAGAATGTCACTAAAATTCTGACAGAGTTTGAACAGCAGTACCGCGTCGTGTTAACTGAATACTATGACAAGGTTAAAGACGAGAAGTTGAGTGATCGCATCTTCTTAATTGACTTAATTCTGTCGGTGAAACACATTGAACGGATTAATGACTATTGCTACAACATCATTAAGGCATTCCTGTTTGTGAAAAACCCAGAAGTCTTTAACTAG", "aa": "MCSRDLERICDYANNLTKFVKHYQHLDVSIFSKLVNLHKSVLNNLKQTFASLQDKEKPLTIQFENVTKILTEFEQQYRVVLTEYYDKVKDEKLSDRIFLIDLILSVKHIERINDYCYNIIKAFLFVKNPEVFN", "aa_hexdigest": "2ef5fac641a11a628cb67912b411cd79", "start_type": "ATG", "rbs_motif": null, "genes": [ "phoU" ], "truncated": "3-prime", "pseudogene": { "start": 730113, "stop": 730790, "observations": { "mutation": [ 730574 ], "stop": [ 730574 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75187", "query_cov": 1.0, "subject_cov": 0.5911111111111111, "identity": 1.0, "score": 264.0, "evalue": 3.38e-88, "gene_start": 1, "gene_end": 133, "reference_start": 93, "reference_end": 225, "reference_sequence": "MESINYQILKRSEKKLLGLFFDYFQHVIKMHETLNKLLCEADVTKREKLIQAIYEMEDFSNKSEFKLINESIWAISKNSPLTNHLRLTITIIMCSRDLERICDYANNLTKFVKHYQHLDVSIFSKLVNLHKSVLNNLKQTFASLQDKEKPLTIQFENVTKILTEFEQQYRVVLTEYYDKVKDEKLSDRIFLIDLILSVKHIERINDYCYNIIKAFLFVKNPEVFN" }, "paralog": false, "identity": 0.9955555555555555, "score": 443.0, "evalue": 5.26e-161, "description": "Internal stop codon at 730574. Nonsense mutation around 730574.", "psc": { "uniref90_id": "UniRef90_P75187", "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "uniref50_id": "UniRef50_P47649", "cog_id": "COG0704", "cog_category": "P", "go_ids": [ "GO:0006817", "GO:0030643", "GO:0045936" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75187", "UniRef:UniRef50_P47649", "COG:COG0704", "COG:P", "GO:0006817", "GO:0030643", "GO:0045936" ] }, "pscc": { "uniref50_id": "UniRef50_P47649", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47649" ], "product": "Uncharacterized protein MG409" } }, "id": "GIHJHMOALN_1289", "locus": "GCA009939745_01289" }, { "type": "cds", "sequence": "contig_1", "start": 730572, "stop": 730790, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGCATTAACTACCAAATTTTAAAGCGTTCAGAAAAAAAGCTGTTAGGACTCTTTTTTGATTACTTTCAGCACGTCATTAAGATGCATGAAACACTCAATAAACTGTTATGTGAAGCGGATGTAACTAAGCGCGAAAAGTTGATTCAAGCAATTTACGAAATGGAAGACTTTTCCAATAAATCGGAGTTTAAACTAATTAACGAATCCATTTGA", "aa": "MESINYQILKRSEKKLLGLFFDYFQHVIKMHETLNKLLCEADVTKREKLIQAIYEMEDFSNKSEFKLINESI", "aa_hexdigest": "ef26c005b503b2183c85d4866faf7786", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8674.006699999994, "isoelectric_point": 6.086455726623535 }, "id": "GIHJHMOALN_1290", "locus": "GCA009939745_01290" }, { "type": "cds", "sequence": "contig_1", "start": 730793, "stop": 731341, "strand": "-", "frame": 3, "gene": "pstB", "product": "phosphate ABC transporter ATP-binding protein PstB", "db_xrefs": [ "COG:COG1117", "COG:P", "EC:7.3.2.1", "GO:0005524", "GO:0005886", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0016887", "GO:0035435", "GO:0055052", "SO:0001217", "UniRef:UniRef50_P47650", "UniRef:UniRef90_P75186" ], "nt": "TTGGGTAAAAACATTAATTCCGGAATTATTAATGATTTAACCTTGCGCACACGGGTTGGTATGGTGTTCCAGCAACTCACCCCTTTTAACTTTTCCATCTTTGAAAACATTGCCTATGGACTGAGAGCCCATGGCATTCATAACAAGCAAGCGATTCATGAAATTGTGGAACAGGCCTTAAAGTCAACCGCCTTATGGGACGAGGTCAAAGATAACTTACACCGTAATGCCAATACCCTTTCGGGTGGTCAACAACAACGGTTGTGTATTGCCAGAGCCATTGCTCTACAACCCGATGTCTTATTAATGGATGAACCAACTAGTGCGTTGGACTCAATTGCCACTAACTCGATTGAGCTGTTAATCCAGCAATTAAAGGAAAAATACACGATTATTATTGTGACTCACTCAATGGCACAAACTATTAGAATCACTGATGAAACGATTTTCTTTGCTAACGGAAGGGTAGTGGAACAAGGTACTACCAAGCAAATCTTTACGAGACCAAAGCAAAAGGAAACTAACCGTTACATTAGTGGGAGGAATTAA", "aa": "MGKNINSGIINDLTLRTRVGMVFQQLTPFNFSIFENIAYGLRAHGIHNKQAIHEIVEQALKSTALWDEVKDNLHRNANTLSGGQQQRLCIARAIALQPDVLLMDEPTSALDSIATNSIELLIQQLKEKYTIIIVTHSMAQTIRITDETIFFANGRVVEQGTTKQIFTRPKQKETNRYISGRN", "aa_hexdigest": "70ff4345f8e9c529a343819ee6637b4f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R5YHY1", "query_cov": 0.9175824175824175, "subject_cov": 0.9881656804733728, "identity": 0.593, "score": 199.0, "evalue": 1.34e-62, "valid": false, "product": "Phosphate ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A836TDX0" }, "genes": [ "pstB" ], "truncated": "3-prime", "pseudogene": { "start": 730793, "stop": 731641, "observations": { "mutation": [ 731362 ], "stop": [ 731362 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75186", "query_cov": 1.0, "subject_cov": 0.5531914893617021, "identity": 0.995, "score": 352.0, "evalue": 1.71e-120, "gene_start": 1, "gene_end": 182, "reference_start": 148, "reference_end": 329, "reference_sequence": "MKKGLKTIWHNFIQKREKVKQYRALYEKQIKQYQQKVAKLDPTTKAEEIANLQSEIDVLQRLIKIKNTKDDVVKQDFDKKNVFEIENLNFWYNKDKQVLFDINLKIKRNKITALIGKSGCGKSTFIRCLNKLNDLNENVRWNGKIFFLGKNINSGIINDLTLRTRVGMVFQQLTPFNFSIFENIAYGLRAHGIHNKQAIHEIVEQALKSTALWDEVKDNLHRNANTLSGGQQQRLCIARAIALQPDVLLMDEPTSALDSIATNSIELLIQQLKEKYTIIIVTHSMAQTIRITDETIFFANGRVVEQGTTKQIFTRPKQKETNRYISGRN" }, "paralog": false, "identity": 0.9964539007092199, "score": 553.0, "evalue": 4.47e-202, "description": "Internal stop codon at 731362. Nonsense mutation around 731362.", "psc": { "uniref90_id": "UniRef90_P75186", "gene": "pstB", "product": "phosphate ABC transporter ATP-binding protein PstB", "ec_ids": [ "7.3.2.1" ], "uniref50_id": "UniRef50_P47650", "cog_id": "COG1117", "cog_category": "P", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0016887", "GO:0035435", "GO:0055052" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75186", "EC:7.3.2.1", "UniRef:UniRef50_P47650", "COG:COG1117", "COG:P", "GO:0005524", "GO:0005886", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0016887", "GO:0035435", "GO:0055052" ] }, "pscc": { "uniref50_id": "UniRef50_P47650", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47650" ], "product": "Phosphate import ATP-binding protein PstB" } }, "id": "GIHJHMOALN_1291", "locus": "GCA009939745_01291" }, { "type": "cds", "sequence": "contig_1", "start": 731360, "stop": 731728, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "GTGAAGCAGTACCGTGCTTTATATGAAAAACAAATTAAGCAATACCAGCAAAAAGTTGCTAAGTTAGATCCCACTACCAAAGCTGAGGAAATTGCTAACTTGCAGAGTGAAATTGATGTGTTGCAACGCCTCATCAAAATTAAGAATACCAAGGACGATGTTGTCAAACAAGACTTTGACAAGAAGAATGTCTTTGAAATTGAAAACCTTAACTTCTGGTACAACAAGGACAAGCAGGTCTTATTTGACATTAACCTCAAAATTAAGCGCAATAAGATTACCGCTTTAATTGGTAAATCCGGTTGTGGTAAGTCCACCTTTATCCGTTGTTTAAATAAATTAAATGACCTCAATGAAAATGTGCGCTGA", "aa": "MKQYRALYEKQIKQYQQKVAKLDPTTKAEEIANLQSEIDVLQRLIKIKNTKDDVVKQDFDKKNVFEIENLNFWYNKDKQVLFDINLKIKRNKITALIGKSGCGKSTFIRCLNKLNDLNENVR", "aa_hexdigest": "c3d9d7c7bda39285a93dd15c52942bc5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 34, "aa_cov": 0.2786885245901639, "hmm_cov": 1.0, "evalue": 3.7747649983633016e-11, "score": 39.25088882446289, "start": 82, "stop": 115 } ], "seq_stats": { "molecular_weight": 14423.573699999977, "isoelectric_point": 9.562031364440916 }, "id": "GIHJHMOALN_1292", "locus": "GCA009939745_01292" }, { "type": "cds", "sequence": "contig_1", "start": 731809, "stop": 732342, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCTCGATTATTTACGGATTGTTTGGTCTATCCTTCTTTTTGCGAACACTGCAATTAAGTGCGGGTGGTGCGAATGGTACTAGTTTAATGGCGGGTATTCTTACTATTAGTGTAGTGGTCTTACCCTTTTTAATTCGCACATGTCAGGAAGCACTCAATAATGTCAGTTGGGACTTGCGGGTAAGTGCTTATGCCTTAGGTGTAAGTAAAAGGGAAGTAATTTGGAAAATTGTGTTACCAGGGGCGTTGAAGGGTTTAATTATTGCGCTTATTTTAACGATTAACAGAATTATTGCGGAAACAGCTCCTTTCTTTATTACCGCTGGGTTAGCATCCAGCAACTTGTTTGATTTAAGTCTACCGGGTCAAACCTTAACCACAAGAATTTATGGTCAGTTATTTTCCACTAACAGTACGGCGGTTGATGTAATGCTGGAAACCGCTTTGGTATCAATTGTCTTCCTGATGTTCTTAATCTTTTTAAGCTCTTACTTAATTCCTTACCTGTTTTCGTTTAATAAACAAAAGTGA", "aa": "MPSIIYGLFGLSFFLRTLQLSAGGANGTSLMAGILTISVVVLPFLIRTCQEALNNVSWDLRVSAYALGVSKREVIWKIVLPGALKGLIIALILTINRIIAETAPFFITAGLASSNLFDLSLPGQTLTTRIYGQLFSTNSTAVDVMLETALVSIVFLMFLIFLSSYLIPYLFSFNKQK", "aa_hexdigest": "842b7b2880b4c45e266f286c90844484", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19304.744499999986, "isoelectric_point": 9.461202812194827 }, "id": "GIHJHMOALN_1293", "locus": "GCA009939745_01293" }, { "type": "cds", "sequence": "contig_1", "start": 732412, "stop": 732588, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGGGATTCTCTTGTTTGTTTTTATTAATGGTAGTCAAGCGATTCAGCGCAGTGGTTCCACTGTCTTTTCGTTTGGTGTAGATACTACAGGGCGAGCTTTAGTCAATACCTTGGTGATTATTCTGGTTGCCATTGGTATTACCTTTCCAATCGCTTTGTTAATTGCCATTTGA", "aa": "MLGILLFVFINGSQAIQRSGSTVFSFGVDTTGRALVNTLVIILVAIGITFPIALLIAI", "aa_hexdigest": "f0a2977fc6b1f3b97b37e326e24a2fff", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6077.2666999999965, "isoelectric_point": 9.3493501663208 }, "id": "GIHJHMOALN_1294", "locus": "GCA009939745_01294" }, { "type": "cds", "sequence": "contig_1", "start": 732634, "stop": 732792, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGGTTTATTCCTAATAACATTGGCGCTCTTTTTACTGATTTAACTGCTAGAAGACAACAGGTTAAAAAGATTACAGCTGCTAATGTTGAGCAGCGCGCTACCTTCTTCAAGGAAAGGATGCAGACTAACCACTTAAATAAGGTTTACACATCGTGA", "aa": "MWFIPNNIGALFTDLTARRQQVKKITAANVEQRATFFKERMQTNHLNKVYTS", "aa_hexdigest": "241db1da5e0dfd144bc01120f48985f2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6128.9974, "isoelectric_point": 10.533698844909669 }, "id": "GIHJHMOALN_1295", "locus": "GCA009939745_01295" }, { "type": "cds", "sequence": "contig_1", "start": 732862, "stop": 733722, "strand": "-", "frame": 1, "gene": "pstA", "product": "phosphate ABC transporter permease PstA", "db_xrefs": [ "COG:COG0581", "COG:P", "GO:0005315", "GO:0005886", "GO:0006817", "GO:0015415", "GO:0035435", "GO:0055052", "SO:0001217", "UniRef:UniRef50_P75185", "UniRef:UniRef90_P75185" ], "nt": "GTGAAACAGAAAATTAAAAGCCGTTTAAAAAAAGACAACTGGCTGCGGTACTTATCGCAAACAGTGGCTGTTTGTTTTTTACTGCTCTTCATTAGCTTCTTTATTTTCCTCTTAATTGAAGCAGCTAAAACTGGTCCTGACTTTACCAAGTCCTTACTGGGTTTGGAATTTAACTTGGGTGCTAAAAAAGCGAGCATCTGGTTTCCGCTGTTAGTTAGTTTTGTGGTTTCGATTGGCTCGTTAATTATTGCGAGCTATATCGGTGTTCGCACTTCGATTTTCTTGGTTTATCGTTGCAAACCCCGGATTCGCAAAAAACTACTTTTAGTAATTGACATTCTGTCCGGGATCCCTTCAGTGATCTTTGGATTGTTTGCTACCCAAATTCTCAGCAGTATCTTCCGTGACGTGTTGCACTTGCCACCCTTATCACTGCTTAACGTGATTGTGATGCTGTCCTTCATGATCATTCCGATTGTGATTTCCTTAACCACCAATGCCTTACTGCACGTGGAATCTAGCTTAATGACGGTTGCCATTTCACTTGGTGAAAACAAAACCAGTGTGATTTATAAGGTAATTAAAAAGGAAATCAAAGCCCAACTAGTAGTAATCTTGGTATTAGCGTTTGGTCGTGCCATTAGCGAAACGATGGCTGTGAACTTTATTTTGCAGAGTGTTAACTACCAGGAAGTGATTGCTAATGATCGTTTCTTTACTTCAGATCTCAAAACTTTGGGTTCTGTTATCTCCACCTTTATCTTTTCGGAAAACGGCGATGAACAGGTTAGTGGGGTCTTATACACTTTTGGCATTATTATCTTTGTCTTAATTTCCTTCTTGAACTTTTTTGCCATTTGA", "aa": "MKQKIKSRLKKDNWLRYLSQTVAVCFLLLFISFFIFLLIEAAKTGPDFTKSLLGLEFNLGAKKASIWFPLLVSFVVSIGSLIIASYIGVRTSIFLVYRCKPRIRKKLLLVIDILSGIPSVIFGLFATQILSSIFRDVLHLPPLSLLNVIVMLSFMIIPIVISLTTNALLHVESSLMTVAISLGENKTSVIYKVIKKEIKAQLVVILVLAFGRAISETMAVNFILQSVNYQEVIANDRFFTSDLKTLGSVISTFIFSENGDEQVSGVLYTFGIIIFVLISFLNFFAI", "aa_hexdigest": "98c1f0a0e0b0ea8e6e84632324317ae2", "start_type": "GTG", "rbs_motif": null, "genes": [ "pstA" ], "truncated": "5-prime", "pseudogene": { "start": 732559, "stop": 733722, "observations": { "mutation": [ 732636, 732864 ], "stop": [ 732636, 732864 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75185", "query_cov": 1.0, "subject_cov": 0.4393241167434716, "identity": 1.0, "score": 506.0, "evalue": 9.16e-175, "gene_start": 1, "gene_end": 286, "reference_start": 1, "reference_end": 286, "reference_sequence": "MKQKIKSRLKKDNWLRYLSQTVAVCFLLLFISFFIFLLIEAAKTGPDFTKSLLGLEFNLGAKKASIWFPLLVSFVVSIGSLIIASYIGVRTSIFLVYRCKPRIRKKLLLVIDILSGIPSVIFGLFATQILSSIFRDVLHLPPLSLLNVIVMLSFMIIPIVISLTTNALLHVESSLMTVAISLGENKTSVIYKVIKKEIKAQLVVILVLAFGRAISETMAVNFILQSVNYQEVIANDRFFTSDLKTLGSVISTFIFSENGDEQVSGVLYTFGIIIFVLISFLNFFAIWSTRPKTLERYPFLKKISNFIYQVVWFIPNNIGALFTDLTARRQQVKKITAANVEQRATFFKERMQTNHLNKVYTSWKILQEIFCAVLAFGFVLGILLFVFINGSQAIQRSGSTVFSFGVDTTGRALVNTLVIILVAIGITFPIALLIAIWLNEYTKSRIAKNTFSFVIDSLSSMPSIIYGLFGLSFFLRTLQLSAGGANGTSLMAGILTISVVVLPFLIRTCQEALNNVSWDLRVSAYALGVSKREVIWKIVLPGALKGLIIALILTINRIIAETAPFFITAGLASSNLFDLSLPGQTLTTRIYGQLFSTNSTAVDVMLETALVSIVFLMFLIFLSSYLIPYLFSFNKQKWLQIKSKLQLWKKA" }, "paralog": false, "identity": 0.9922480620155039, "score": 728.0, "evalue": 1.81e-264, "description": "Internal stop codon at 732636, 732864. Nonsense mutation around 732636, 732864.", "psc": { "uniref90_id": "UniRef90_P75185", "gene": "pstA", "product": "phosphate ABC transporter permease PstA", "uniref50_id": "UniRef50_P75185", "cog_id": "COG0581", "cog_category": "P", "go_ids": [ "GO:0005315", "GO:0005886", "GO:0006817", "GO:0015415", "GO:0035435", "GO:0055052" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75185", "UniRef:UniRef50_P75185", "COG:COG0581", "COG:P", "GO:0005315", "GO:0005886", "GO:0006817", "GO:0015415", "GO:0035435", "GO:0055052" ] }, "pscc": { "uniref50_id": "UniRef50_P75185", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75185" ], "product": "Phosphate transport system permease protein PstA homolog" } }, "id": "GIHJHMOALN_1296", "locus": "GCA009939745_01296" }, { "type": "cds", "sequence": "contig_1", "start": 733722, "stop": 733898, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB5478A", "UniRef:UniRef90_UPI001FB5478A" ], "nt": "TTGTTATTTAGCAACAACAGTGAAATTAAGAAAATATACGATGACTTTGGTGTGTTAGAGTTAACGGCTGACGAAAAGAAAAAGATGTTTAAAACAGGTAATGCAGAAATGAGCAACATTGCCAACTTCTGGGTTGATGATTACAGTCTGAACAACCAAACCTTCGGTGCACTCTAG", "aa": "MLFSNNSEIKKIYDDFGVLELTADEKKKMFKTGNAEMSNIANFWVDDYSLNNQTFGAL", "aa_hexdigest": "db872504cdb65525c719520b4b55ed97", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F234BB1", "query_cov": 1.0, "subject_cov": 0.8529411764705882, "identity": 0.569, "score": 71.2, "evalue": 3.43e-15, "valid": false, "uniref50_id": "UniRef50_UPI001FB5478A" }, "pscc": { "uniref50_id": "UniRef50_UPI001FB5478A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB5478A" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 733722, "stop": 733967, "observations": { "mutation": [ 733901 ], "stop": [ 733901 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FB5478A", "query_cov": 1.0, "subject_cov": 0.7160493827160493, "identity": 0.983, "score": 118.0, "evalue": 8.42e-34, "gene_start": 1, "gene_end": 58, "reference_start": 24, "reference_end": 81, "reference_sequence": "MNSVVSLLPKQKTLPSIQRFFNWLLFSNNSEIKKIYDDFGVLELTADEKKKMFKTGNAEMSNIANFWVDDYSLNNQTFGAL" }, "paralog": false, "identity": 0.9753086419753086, "score": 159.0, "evalue": 1.77e-52, "description": "Internal stop codon at 733901. Nonsense mutation around 733901.", "psc": { "uniref90_id": "UniRef90_UPI001FB5478A", "uniref50_id": "UniRef50_UPI001FB5478A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FB5478A", "UniRef:UniRef50_UPI001FB5478A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FB5478A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB5478A" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6658.412000000002, "isoelectric_point": 4.563057136535646 }, "id": "GIHJHMOALN_1297", "locus": "GCA009939745_01297" }, { "type": "cds", "sequence": "contig_1", "start": 733899, "stop": 734879, "strand": "-", "frame": 2, "gene": "pstS", "product": "putative lipo MG412-like protein", "db_xrefs": [ "COG:COG0226", "COG:P", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47652", "UniRef:UniRef90_P75184" ], "nt": "ATGCCAGTTTTTCTAAAATTAACGCACACAATTAGAAAAGTGCTAAGAGTTGCAAGACTTTCTAGACTAGCATTACTATCACTAACCGCTGTTATTTTTAGCGGTTGTGCCAATATTAATTTAATTAGTGCTGTTGGTTCTTCTTCGGTACAACCGTTGTTAAGCAAACTCAGTTCGCACTATGTCTTGAACCACAATGACAAGGATAACCTTGTAGAAATTAGTGTCCAAGCGGGTGGCTCTAGTGCTGGGGTGAAAGCAATCACCAAGGGACTAGCTGACATTGGTAATGTCTCGAAAAACACCAAGAGCTATGCTGAGGAAAACAAGCAGTTGTGGATGGACAAAAAGCTCAAAACAATTACACTTGGCAAAGATGCCATTGCTGTTATTTATAAAGCACCATCAGAGTTTAAGGGCAAACTAGTTCTCACTAAAGACAACCTCAACGATCTTTACGATCTGTTCGCTGGTAGCAAAAGCGTTGACATTAATAAGTTTGTCGAAAACGGACAAACCACTAAAAACAGTAATCATAATTTGATAGGCTTCCCCCGTACTGGGGGCGCTTTTGCTTCTGGTACCGCTGAAGCTTTCTTGAAGTTTTCGGGTCTTACACAGACAAAAACTTTAGATAAAGATTCCAAAGAAATTTTGGAAGGTCAACGCAACTATGGCCCCAATGCGCGACCAACTAGTGAAACCAACATTGAGGCCTTTAATACCTTTGTCACAACTTTGCGACAACCCAATTTATACGGCATGGTGTACCTCAGTTTAGGGTTTGTGAATAACAACATGAACCTAATTAAGAGTGAAGGTTTTGAGGTTTTAAAAGTCAAATATGATAATAACGCAGTTACCCCCTCCAGTCAAGCAGTTTCTAGCAACACTTACAAATGGGTACGCCCGTTGAACTCAGTGGTTTCCCTGTTACCAAAACAAAAAACACTGCCAAGTATCCAACGCTTTTTTAACTGA", "aa": "MPVFLKLTHTIRKVLRVARLSRLALLSLTAVIFSGCANINLISAVGSSSVQPLLSKLSSHYVLNHNDKDNLVEISVQAGGSSAGVKAITKGLADIGNVSKNTKSYAEENKQLWMDKKLKTITLGKDAIAVIYKAPSEFKGKLVLTKDNLNDLYDLFAGSKSVDINKFVENGQTTKNSNHNLIGFPRTGGAFASGTAEAFLKFSGLTQTKTLDKDSKEILEGQRNYGPNARPTSETNIEAFNTFVTTLRQPNLYGMVYLSLGFVNNNMNLIKSEGFEVLKVKYDNNAVTPSSQAVSSNTYKWVRPLNSVVSLLPKQKTLPSIQRFFN", "aa_hexdigest": "2d738190940de6080edb27631c107cab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75184", "query_cov": 1.0, "subject_cov": 0.8467532467532467, "identity": 1.0, "score": 620.0, "evalue": 3.3e-223, "valid": true, "gene": "PstS", "product": "Uncharacterized lipoprotein MG412 homolog", "uniref50_id": "UniRef50_P47652", "cog_id": "COG0226", "cog_category": "P", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47652", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47652" ], "product": "Uncharacterized lipoprotein MG412" }, "genes": [ "pstS" ], "id": "GIHJHMOALN_1298", "locus": "GCA009939745_01298" }, { "type": "cds", "sequence": "contig_1", "start": 735121, "stop": 736068, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6450", "UniRef:UniRef100_A0AAX0SPZ2", "UniRef:UniRef50_A0AAX0SPZ2", "UniRef:UniRef90_A0AAX0SPZ2" ], "nt": "ATGGAAAAACTCAATGATTTTGAAAAGCAACAAATTGTTTTTGTCATTAAAAACAGTTTTGCTGGTGTTGATAAAACCGTGTTAGATGTAGATGCTAACAGCTTAACCTTTGTGGAAGATCCCCGACAATACAAACTTGATTTAGGAAACATGAACGAAAACAAAGGTTTCTTCTATGTGTTAGCCGACATCCACAACAGCAACGAACTGTTTAAGTTAGGTTCCACGATCGATCCCGAAATTATAAAAGGGCAAATGTACTTTGTTAAACAAAATCAAATAAAGCCCAACACACGCACTTACTTGTTCAAAATTAATACGCGCAAGCTGTTGCTTGACAAACAAAAGTTGTGGTTTAAACCCCACTTGGAAACCCAAACAGTGAGCTTTATGTTTGATGAATTCGAAATTGGCAAATTAGATATAGAAACATCCAACATCCAATTAATAGATGACTATGAGTTTGAGTTTCAATCAGACTTTAGTTTGCAAGATGAACAAGTGTTATTGGAAAAACTCAACATTGTTTTGAGTCAAATGAACCTCCATGTGGAAACGAAGAATGCTAGGTTGAATCCGGTAAATAAAATGGCTTATTTAACCGTTTTAAAAAACAACCAAAAGTTCAACTTAGAGTTCCATGTTGATCGCTATACTAGTCAGCTCTCCATTCAAGCAATGTTGGGTAGTCAAAGACTTTTTGCAATTAACTATGACTTAAAGCTATCACAAAATAAGCAAATGCTATATCTCATTAATAAAGATGGTCTAGAAAAGTCAGTTTGGTTCAACATTAAAAATGACAGTCAAACTAAGCTAGCACAACAACTAGCTAGATTCTTAAAGGAAAACAACTTCCAGTTTAGACAAAAACCAGTCTTTGATTTCCACACCCAAAACAAAGCCTTTGCACTGGAAAAGTTGGACAATAAAGATGACACTGATTAA", "aa": "MEKLNDFEKQQIVFVIKNSFAGVDKTVLDVDANSLTFVEDPRQYKLDLGNMNENKGFFYVLADIHNSNELFKLGSTIDPEIIKGQMYFVKQNQIKPNTRTYLFKINTRKLLLDKQKLWFKPHLETQTVSFMFDEFEIGKLDIETSNIQLIDDYEFEFQSDFSLQDEQVLLEKLNIVLSQMNLHVETKNARLNPVNKMAYLTVLKNNQKFNLEFHVDRYTSQLSIQAMLGSQRLFAINYDLKLSQNKQMLYLINKDGLEKSVWFNIKNDSQTKLAQQLARFLKENNFQFRQKPVFDFHTQNKAFALEKLDNKDDTD", "aa_hexdigest": "9c3d4f3fdc80a0549b0ed8a661e43592", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6450", "uniref100_id": "UniRef100_A0AAX0SPZ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6450", "UniRef:UniRef100_A0AAX0SPZ2" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SPZ2", "uniref90_id": "UniRef90_A0AAX0SPZ2" }, "psc": { "uniref90_id": "UniRef90_A0AAX0SPZ2", "uniref50_id": "UniRef50_A0AAX0SPZ2" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SPZ2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37164.97450000004, "isoelectric_point": 6.034732246398924 }, "id": "GIHJHMOALN_1299", "locus": "GCA009939745_01299" }, { "type": "cds", "sequence": "contig_1", "start": 736135, "stop": 736350, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACACCACAACAACACGCTTCCAACGAATACACCATTAACTTTTTAAGTTTGGATTTTATTGTTGACGGTTATGAATCAGAAAACTTGCACTTCCAAGTAGTTGATCAACTTTTTGGTGCAGTGAAAAACCTACCAACGATCAACAAAATTCCAGAAGGTTACCAGTACAAATTTAAATATCGCAGTAACTTTAGCAACCAGCAATTTAAATAA", "aa": "MTPQQHASNEYTINFLSLDFIVDGYESENLHFQVVDQLFGAVKNLPTINKIPEGYQYKFKYRSNFSNQQFK", "aa_hexdigest": "5c399516d4654f89bf44a7bf34839b3b", "start_type": "TTG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8382.256399999998, "isoelectric_point": 6.018874168395995 }, "id": "GIHJHMOALN_1300", "locus": "GCA009939745_01300" }, { "type": "cds", "sequence": "contig_1", "start": 736360, "stop": 736758, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATACTCTGTCCAAAACATTTAATGAAAAAGATCTATCTCAAAAAGATCAATTACAAATAATGTTCAAAAAGGAGTTTACTCAAGCGATTGCCCAAATTAGTAAGTTGAACGAAGAGATTGATTTAGCTACTGATAAAGCAACAGCGCTACAAAAGTTTGACTCTTTAAAGTCCAAGGACAGCAACGTTAATTTTGCTAGCTTGCAGTACCTCTATGACTTATTAGGGATGGAGCCTACCAATAAAGAACACCTTATCTTCATTAGGAACTTACCAGAGATGTTGCAAACTATTTTTGCACGAGCCAAACTTTTAGCTAAAGTCAAAATTGGTGATACCTTGCAAGAAATTACGTTGCTTAAAAACAATGTCAATGTGTTTGATATCAAAACTTGA", "aa": "MDTLSKTFNEKDLSQKDQLQIMFKKEFTQAIAQISKLNEEIDLATDKATALQKFDSLKSKDSNVNFASLQYLYDLLGMEPTNKEHLIFIRNLPEMLQTIFARAKLLAKVKIGDTLQEITLLKNNVNVFDIKT", "aa_hexdigest": "3fa208e72ec2bab3245796d2e4678c48", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15157.384399999984, "isoelectric_point": 6.5873208999633786 }, "id": "GIHJHMOALN_1301", "locus": "GCA009939745_01301" }, { "type": "cds", "sequence": "contig_1", "start": 736792, "stop": 737034, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCCTTTACTAGAAACCGCCAAGTAACCCAATCAATCTTGAATATGGGTAAAAACGTTGTAGTAAAGCCATTAACTGATGATGAGGAACAAAGTTTGTTTAGCAATAAGTGGGACATTTTCAAATACACCCCAATTATCCCAGTAAACGCTCAGCGCTTTGGTGTTGACTTTTATGTGGAACAACATAATGCAGATCTAAAACCACCTATTAGTTTTAGTCAACTTTCTAGGTTGTGA", "aa": "MEAFTRNRQVTQSILNMGKNVVVKPLTDDEEQSLFSNKWDIFKYTPIIPVNAQRFGVDFYVEQHNADLKPPISFSQLSRL", "aa_hexdigest": "898462f945d8a76462aab728de47360b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9302.476099999996, "isoelectric_point": 6.54571475982666 }, "id": "GIHJHMOALN_1302", "locus": "GCA009939745_01302" }, { "type": "cds", "sequence": "contig_1", "start": 737526, "stop": 737822, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HPZ9", "UniRef:UniRef90_A0AB33HPZ9" ], "nt": "TTGCTTAGAGAAAATAACTTTAATTGGTATAACAATATCTATTACATCAAAGATGTTAACTTAGGCATTGAAAGTAGCTATACTAGCAGTTTGGCATGGAACAAAACTTTTAGCTATCCCGGAGTTAGAAACTCCTTTAAATCAGATATACACGACCTTCAGTATTTCCACTTTCACAATGATAGTAAGTCTGGTTTTGGACCATCTCGCATTAATGACCCGTGGTATCCACAGCATGATAAATATGTGACATTAAGCGCTGGTCGAACTGCCGAAGAAGCTAAAAACGCAGGATGA", "aa": "MLRENNFNWYNNIYYIKDVNLGIESSYTSSLAWNKTFSYPGVRNSFKSDIHDLQYFHFHNDSKSGFGPSRINDPWYPQHDKYVTLSAGRTAEEAKNAG", "aa_hexdigest": "7a72347229b26f1751ab42b255f19204", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 737397, "stop": 738113, "observations": { "mutation": [ 737432, 737528, 737864, 737882 ], "stop": [ 737432, 737528, 737864, 737882 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HPZ9", "query_cov": 1.0, "subject_cov": 0.4117647058823529, "identity": 0.99, "score": 207.0, "evalue": 2.76e-66, "gene_start": 1, "gene_end": 98, "reference_start": 98, "reference_end": 195, "reference_sequence": "MRSYHYLPLLGFVAFPTLLANASLDKNLVDSSLYISNSAHHNQGFTVGIKLKDSYFRKDFNFENPLIYKPNNKTTFGWNYSTLWTQLADGYYDRYNQLLRENNFNWYNNIYYIKDVNLGIESSYTSSLAWNKTFSYPGVRNSFKSDIHDLQYFHFHNDSKSGFGPSRINDPWYPQHDKYVTLSAGRTAEEAKNAGWSIPYNLETQRLFSPVWNRNKDFKWINVFSVLWTVPYQLSHLT" }, "paralog": false, "identity": 0.9831932773109243, "score": 488.0, "evalue": 2.28e-179, "description": "Internal stop codon at 737432, 737528, 737864, 737882. Nonsense mutation around 737432, 737528, 737864, 737882.", "psc": { "uniref90_id": "UniRef90_A0AB33HPZ9", "uniref50_id": "UniRef50_A0AB33HPZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HPZ9", "UniRef:UniRef50_A0AB33HPZ9" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HPZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HPZ9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11434.36359999999, "isoelectric_point": 6.899025917053222 }, "id": "GIHJHMOALN_1303", "locus": "GCA009939745_01303" }, { "type": "cds", "sequence": "contig_1", "start": 737880, "stop": 738113, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCTCATATCATTATTTACCTTTATTAGGTTTTGTTGCGTTCCCAACTCTTTTAGCGAACGCGTCGTTGGATAAAAATCTTGTTGATAGTAGTTTGTATATTTCTAACTCAGCACACCACAATCAAGGGTTTACTGTTGGGATCAAATTAAAGGATAGTTATTTCCGTAAAGACTTTAATTTCGAAAATCCTTTAATTTATAAACCAAATAACAAAACTACTTTTGGTTGA", "aa": "MRSYHYLPLLGFVAFPTLLANASLDKNLVDSSLYISNSAHHNQGFTVGIKLKDSYFRKDFNFENPLIYKPNNKTTFG", "aa_hexdigest": "ec624bcf87c076c6f7c13776da001dba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8801.906699999992, "isoelectric_point": 9.347158241271973 }, "id": "GIHJHMOALN_1304", "locus": "GCA009939745_01304" }, { "type": "cds", "sequence": "contig_1", "start": 738228, "stop": 738890, "strand": "-", "frame": 2, "gene": null, "product": "putative MG415-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75182", "UniRef:UniRef90_P75182" ], "nt": "TTGCCTAACGTTGCTTTCTTTAACGATTACCAAGTAGATTTGGTTTCTACCTTAACGTTAGCTGATAAGCAACTTTTAATAAGAAGACTGAACCTTGCATTAGTTGGCATGAACTTAATGGTTGATGAACAAGCTACTAACATAGAAACTTTTCCCAAACAAATAAGACTCACTACAAAGTCAATAGATCAATCGTTTGATTTTGATGTTGAATTTAATCCAAAAGCTAGTGAACTTAATGTAATTGTTAAGGTTGACGAACAACCATTATTTAAGTTGGATTATGAACTAGTGATTAAAAATTCAAACACTCTACAACTTGTTAACCGCAACAAGCATTTAGGCATGTATATCTGGTCTGTTGCTGATCAACGATACCAAGCTAAATTGCTCGATACCTTGCAGTTATTTCTTCAAAGTAAGCAGATGTACTTAAAAGAAAAGATTCCGCTTAAACTGCAGGATAATACCGCAACTTTTAAAATCGCCAAACAGGCTCAACCAAATAATGGTGAAAATAAAAACACCAATTTTATTGGTTACTTAGTAATTGCTGCTAGTAGTCTGTTTTTAGTGGTAGTTGCTTTTAGTTTTTACTTTTACAAGCGCAAAAAGAGTTTGAAATCGCACAAACAAAAAGCAGTTGTCAAAACGGATAAATAA", "aa": "MPNVAFFNDYQVDLVSTLTLADKQLLIRRLNLALVGMNLMVDEQATNIETFPKQIRLTTKSIDQSFDFDVEFNPKASELNVIVKVDEQPLFKLDYELVIKNSNTLQLVNRNKHLGMYIWSVADQRYQAKLLDTLQLFLQSKQMYLKEKIPLKLQDNTATFKIAKQAQPNNGENKNTNFIGYLVIAASSLFLVVVAFSFYFYKRKKSLKSHKQKAVVKTDK", "aa_hexdigest": "c475fda39bb4f122e2c544a38bee10f7", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 738228, "stop": 739190, "observations": { "mutation": [ 738962, 739097 ], "stop": [ 738962, 739097 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75182", "query_cov": 1.0, "subject_cov": 0.6395348837209303, "identity": 0.991, "score": 414.0, "evalue": 6.13e-144, "gene_start": 1, "gene_end": 220, "reference_start": 125, "reference_end": 344, "reference_sequence": "MVFSFQDDTALTVALNSIKLVKNPEELIPGLIPTNPKTGIFYIAAEITNSNNFFKWGTDSNREIIKGQMYFLKKETKIPTIRTYLFQMRTHKIALNANDVWFQANNDKVKVIVDGIELDQFDPKLPNVAFFNDYQVDLVSTLTLADKQLLIRRLNLALVGMNLMVDEQATNIETFPKQIRLTTKSIDQSFDFDVEFNPKASELNVIVKVDEQPLFKLDYELVIKNSNTLQLVNRNKHLGMYIWSVADQRYQAKLLDTLQLFLQSKQMYLKEKIPLKLQDNTATFKIAKQAQPNNGENKNTNFIGYLVIAASSLFLVVVAFSFYFYKRKKNLKSHKQKAVVKTDK" }, "paralog": false, "identity": 0.990625, "score": 620.0, "evalue": 1.1e-227, "description": "Internal stop codon at 738962, 739097. Nonsense mutation around 738962, 739097.", "psc": { "uniref90_id": "UniRef90_P75182", "product": "Uncharacterized protein MG415 homolog", "uniref50_id": "UniRef50_P75182", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75182", "UniRef:UniRef50_P75182" ] }, "pscc": { "uniref50_id": "UniRef50_P75182", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75182" ], "product": "Uncharacterized protein MG415 homolog" } }, "id": "GIHJHMOALN_1305", "locus": "GCA009939745_01305" }, { "type": "cds", "sequence": "contig_1", "start": 738960, "stop": 739058, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACTTTCTCAAAAAAGAAACAAAGATTCCAACAATTCGTACTTACTTGTTTCAAATGAGAACACACAAAATTGCTTTGAATGCCAATGACGTTTGA", "aa": "MYFLKKETKIPTIRTYLFQMRTHKIALNANDV", "aa_hexdigest": "f55ad7917052de9d645a5a467e9e5e81", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3885.6002000000008, "isoelectric_point": 9.99467868804932 }, "id": "GIHJHMOALN_1306", "locus": "GCA009939745_01306" }, { "type": "cds", "sequence": "contig_1", "start": 739095, "stop": 739262, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTTTCATTTCAGGATGATACGGCATTAACTGTTGCCTTAAATTCAATCAAATTAGTTAAAAATCCTGAAGAACTAATTCCTGGTTTAATTCCGACAAATCCTAAAACGGGCATCTTCTATATTGCAGCAGAAATTACTAACAGCAATAATTTCTTTAAATGA", "aa": "MVFSFQDDTALTVALNSIKLVKNPEELIPGLIPTNPKTGIFYIAAEITNSNNFFK", "aa_hexdigest": "7d2cfff18e9a15ac49bde0fbe0e257d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6086.957300000002, "isoelectric_point": 4.93575038909912 }, "id": "GIHJHMOALN_1307", "locus": "GCA009939745_01307" }, { "type": "cds", "sequence": "contig_1", "start": 739240, "stop": 739488, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTCTTTATTTGAATCTGCTAAAAATAAACCAACCATTCAAAAAACAAACCAGGGCATTCAATACCGTTTAAGGTTTCGCTCAAATTACAACGAACAGTATTTCAATAAATATCGTTTTAGCATTCCCGTCGTTAATAACGGCAAGGAGTTTAGCGTTTTAGACATCCAAGATAAAGAATTGACTGAAGAGCAGAAAAACCAAATAGCTTTTGTAATTAAAAATGGTTTTTTCATTTCAGGATGA", "aa": "MFSLFESAKNKPTIQKTNQGIQYRLRFRSNYNEQYFNKYRFSIPVVNNGKEFSVLDIQDKELTEEQKNQIAFVIKNGFFISG", "aa_hexdigest": "37a9782533401d3c2661c13c76776856", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9736.900599999997, "isoelectric_point": 9.520900535583493 }, "id": "GIHJHMOALN_1308", "locus": "GCA009939745_01308" }, { "type": "cds", "sequence": "contig_1", "start": 739507, "stop": 739668, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGGGCAATTAAGAAAGATTACTTTAGTTAAAAACAACAATAACGTTTTTAATCTAGGCCAGTTTGAACAATTCTTAAATTTAGATCAAATTAGCATTAATTTATTTGAAGTTGAATTTCTCTCTTTTGATTTCATATCAGATCAATATCCTAGTTGA", "aa": "MGGQLRKITLVKNNNNVFNLGQFEQFLNLDQISINLFEVEFLSFDFISDQYPS", "aa_hexdigest": "f92003b0e74c2a2f68530ec69285d987", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6186.908599999997, "isoelectric_point": 4.295857048034668 }, "id": "GIHJHMOALN_1309", "locus": "GCA009939745_01309" }, { "type": "cds", "sequence": "contig_1", "start": 739801, "stop": 740175, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17BD43", "UniRef:UniRef90_UPI001F17BD43" ], "nt": "ATGGGTTTTTCATTAACCCCACTGTTGCTCTCTAGCGCAACTATCAATTCACATGTAATTGACAGCTCGTTTTACTTAACAAAAAATTTAGTAGAGAGCGAAAATCAATCACCTATTCAGTTCAATCAGCTATCGCGAATTATTTTAGTTCAATTAGAGTTTGACCCTGATTCAATAGTTGATAATACTTCTATCACCTTTAATAACCGAACGCTTGGCCAAAAAGCTCTTTTGAAATTACGTTTTAAAAGGGAATTTACCTTAGCGATTCAAGAAATTACAGAGCTTAATCAGTTAGTTGATCAAGCAATTGATAAAAATACAGTTTTACAAAATTTCTTAACGCTTAAAAATGTTGAACGAAAACAGCAATGA", "aa": "MGFSLTPLLLSSATINSHVIDSSFYLTKNLVESENQSPIQFNQLSRIILVQLEFDPDSIVDNTSITFNNRTLGQKALLKLRFKREFTLAIQEITELNQLVDQAIDKNTVLQNFLTLKNVERKQQ", "aa_hexdigest": "19607bc6a64832d6d7eace8082e8d799", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 739507, "stop": 740220, "observations": { "mutation": [ 739803, 740184 ], "stop": [ 739803, 740184 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F17BD43", "query_cov": 1.0, "subject_cov": 0.5232067510548524, "identity": 1.0, "score": 234.0, "evalue": 1.8e-76, "gene_start": 1, "gene_end": 124, "reference_start": 16, "reference_end": 139, "reference_sequence": "MNVKIAMPFLKYWKLMGFSLTPLLLSSATINSHVIDSSFYLTKNLVESENQSPIQFNQLSRIILVQLEFDPDSIVDNTSITFNNRTLGQKALLKLRFKREFTLAIQEITELNQLVDQAIDKNTVLQNFLTLKNVERKQQWERLSYLYKLLNFDFRDPQELSLVRDLPRLLKTIFESASINFQVRMGGQLRKITLVKNNNNVFNLGQFEQFLNLDQISINLFEFEFLSFDFISDQYPS" }, "paralog": false, "identity": 0.9831223628691983, "score": 448.0, "evalue": 6.02e-163, "description": "Internal stop codon at 739803, 740184. Nonsense mutation around 739803, 740184.", "psc": { "uniref90_id": "UniRef90_UPI001F17BD43", "uniref50_id": "UniRef50_UPI001F17BD43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F17BD43", "UniRef:UniRef50_UPI001F17BD43" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F17BD43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F17BD43" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14198.057499999977, "isoelectric_point": 5.770260429382325 }, "id": "GIHJHMOALN_1310", "locus": "GCA009939745_01310" }, { "type": "cds", "sequence": "contig_1", "start": 740288, "stop": 740485, "strand": "-", "frame": 3, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF615P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniParc:UPI0001DD6453", "UniRef:UniRef100_A0AB38W6H7", "UniRef:UniRef50_P75435", "UniRef:UniRef90_P75180" ], "nt": "ATGGCTGAAATTGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCTGAAATCGAAATGCGCAAAAAGCAATTGGATTATTACTACCATTTAATCTTTAGCAAGATTGCTCACTTTTCCAAACAATTAGCTTAG", "aa": "MAEIELSFPPLEIQEKIADILFAFEKLCNDLVEGIPAEIEMRKKQLDYYYHLIFSKIAHFSKQLA", "aa_hexdigest": "977700c6647ca33d66291832bb7844ef", "start_type": "ATG", "rbs_motif": "AAAA", "ups": { "uniparc_id": "UPI0001DD6453", "uniref100_id": "UniRef100_A0AB38W6H7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6453", "UniRef:UniRef100_A0AB38W6H7" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6H7", "uniref90_id": "UniRef90_P75180" }, "psc": { "uniref90_id": "UniRef90_P75180", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF615P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_P75435", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_P75435", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75435" ], "product": "Type I restriction enzyme MpnII specificity subunit" }, "genes": [ "hsdS" ], "id": "GIHJHMOALN_1311", "locus": "GCA009939745_01311" }, { "type": "cds", "sequence": "contig_1", "start": 740944, "stop": 741036, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "ATGCAAGGAATTTTAGCAGAAATTGAGCTAGATTTCCCACCTTTGCAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTCCGAGCTAA", "aa": "MQGILAEIELDFPPLQIQEKIATILDTFPS", "aa_hexdigest": "d6c3b8b447a38b294f3c45cb5f0eefd9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 26, "aa_cov": 0.8666666666666667, "hmm_cov": 1.0, "evalue": 1.2126773881133658e-06, "score": 24.307828903198242, "start": 3, "stop": 28 } ], "seq_stats": { "molecular_weight": 3371.8918000000003, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_1312", "locus": "GCA009939745_01312" }, { "type": "cds", "sequence": "contig_1", "start": 741214, "stop": 741393, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGTTGATTAAACGTTTCAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAACTATATATCAAAAACAATCCAGGAGAATTCCCTGTTTATTCATCAGCAACAGTTAATAACGGTGAAATCGGTAAGATAAAGGATTGTGATTTTAATGGAGAGTACGTCACTTGA", "aa": "MLIKRFKIKDICDIKRGRVISKLYIKNNPGEFPVYSSATVNNGEIGKIKDCDFNGEYVT", "aa_hexdigest": "148aa3d4c075baf84a3b0da6e83ab4d5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 60, "aa_cov": 0.9491525423728814, "hmm_cov": 0.9833333333333333, "evalue": 4.667390072324948e-11, "score": 38.6718864440918, "start": 4, "stop": 59 } ], "seq_stats": { "molecular_weight": 6740.782700000003, "isoelectric_point": 9.263607215881347 }, "id": "GIHJHMOALN_1313", "locus": "GCA009939745_01313" }, { "type": "cds", "sequence": "contig_1", "start": 741466, "stop": 741864, "strand": "-", "frame": 1, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627", "KEGG:K02996", "RefSeq:WP_014325637.1", "SO:0001217", "UniParc:UPI0001DD6454", "UniRef:UniRef100_A0AAV5N9T9", "UniRef:UniRef50_Q98Q72", "UniRef:UniRef90_P75179" ], "nt": "ATGGAAAAACAATCTTATTATGGACTTGGACGCCGTAAGTCTTCAAGTGCTAAAGTTTATCTAACGCCTACCCAAGACAAGGGTAAGATTACTGTTAACCGTCGTGATCCTAGTGAATACTTTCCCAATAAAATAGTGATCCAAGACATGGAACAACCTTTAGATCTCACTGATCTTAAAAAGAACTTTGATATCAATGTGGTAGTTAAAGGTGGTGGTTTTACCGGACAAGCTGGTGCAATTCGTTTAGGAATTGTTCGCGCGCTTTTACAGTTCAATCCAGAATTGAAAAAGATTCTCAAGTCCAAGAAACTAACTACCCGTGACAAGCGGGTTAAAGAACGGAAGAAGTTCGGTTTATACGGTGCCCGCCGTGCTCCACAATTTACCAAGCGTTAG", "aa": "MEKQSYYGLGRRKSSSAKVYLTPTQDKGKITVNRRDPSEYFPNKIVIQDMEQPLDLTDLKKNFDINVVVKGGGFTGQAGAIRLGIVRALLQFNPELKKILKSKKLTTRDKRVKERKKFGLYGARRAPQFTKR", "aa_hexdigest": "8710d2521cbc32120e7a560a0cc6fdf7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6454", "ncbi_nrp_id": "WP_014325637.1", "uniref100_id": "UniRef100_A0AAV5N9T9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6454", "RefSeq:WP_014325637.1", "UniRef:UniRef100_A0AAV5N9T9" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N9T9", "uniref90_id": "UniRef90_P75179" }, "psc": { "uniref90_id": "UniRef90_P75179", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q98Q72", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q72" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "GIHJHMOALN_1314", "locus": "GCA009939745_01314" }, { "type": "cds", "sequence": "contig_1", "start": 741948, "stop": 742310, "strand": "-", "frame": 2, "gene": "rplM", "product": "50S ribosomal protein L13", "db_xrefs": [ "COG:COG0102", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625", "KEGG:K02871", "SO:0001217", "UniRef:UniRef50_P47657", "UniRef:UniRef90_P75178" ], "nt": "ATGCAAAAAACATCAATGCTAACAAAAGAACAGGCCAACAAGCGACGACAGTGGTATATTGTCGACGCTGCTGGGCTCGTTCTCGGTAAGTTAGCGGTTAAAGCGGCCGATCTTATTCGCGGCAAGAATAAAGTTGATTTCACCCCTAACCAAGATTGTGGTGATTACTTGATCATTATTAACAGTGATCAAGTTGTGTTGACTGGCAATAAAAAAGAAAACGAGTTTTGGTACCACCACTCCCAATACATTGGTGGCATTAAGAAGGTAAGTGGTCGTGATATGTTGAAAAAACAATCCGACAAACTGGTCTATAATGCTGTTAAGGGCATGTTACCTGATAACCGTTTAAGCCGTCGTTGA", "aa": "MQKTSMLTKEQANKRRQWYIVDAAGLVLGKLAVKAADLIRGKNKVDFTPNQDCGDYLIIINSDQVVLTGNKKENEFWYHHSQYIGGIKKVSGRDMLKKQSDKLVYNAVKGMLPDNRLSRR", "aa_hexdigest": "2cd7e8a41898bbd90dc239a949ba520f", "start_type": "ATG", "rbs_motif": "AATAA", "psc": { "uniref90_id": "UniRef90_P75178", "query_cov": 1.0, "subject_cov": 0.821917808219178, "identity": 1.0, "score": 243.0, "evalue": 3.32e-81, "valid": true, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_P47657", "cog_id": "COG0102", "cog_category": "J", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47657" ], "product": "Large ribosomal subunit protein uL13" }, "genes": [ "rplM" ], "id": "GIHJHMOALN_1315", "locus": "GCA009939745_01315" }, { "type": "cds", "sequence": "contig_1", "start": 742341, "stop": 742562, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAAATTTTTCAACAAAACCTGTTCTTGAGTTCCTTTACTTTGAGTGAATATGAAACTAAGGTACTAGCTAAAGTGGAACAACTAACGCATAAACCCCAAGTGTTGCAATTACAACAGTTAGAACAGTTAAGTAGTACCGTTGTTAAAAAAGCTCAAAAAACAGCGGCACAGAAGATCGCTGATACGTTCTTCAAAGGGCTTTATGAAGAAAAGTAA", "aa": "MLEIFQQNLFLSSFTLSEYETKVLAKVEQLTHKPQVLQLQQLEQLSSTVVKKAQKTAAQKIADTFFKGLYEEK", "aa_hexdigest": "babae3458941ed446fb1fc0c1df2d9e7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8434.6664, "isoelectric_point": 7.986166191101074 }, "id": "GIHJHMOALN_1316", "locus": "GCA009939745_01316" }, { "type": "cds", "sequence": "contig_1", "start": 742599, "stop": 744386, "strand": "-", "frame": 2, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "COG:COG2812", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872", "KEGG:K02343", "SO:0001217", "UniRef:UniRef50_P47658", "UniRef:UniRef90_P75177" ], "nt": "ATGCGCAAGGTCCTTTACCAGAAATACCGTCCCACCAAGTTTAGCGATACTGTCGGTCAGGACAGCATTAAACGCATTATTGTTAATGCCATTACCCAAGACCAATTACCCCACGGTTACATCTTTGCGGGGGAGCGGGGTACGGGAAAAACGACCTTTGCCAAGATTATTGCCAAAGCCATTAACTGTTTAAACTGGAACGGCGATGTTTGCAACCAGTGTGAGGCATGCCAAGCGATTAACAGCAACAGTGCGATTGATGTGTTTGAAATTGATGCTGCTTCCAAAAACGGGATTAACGACATTAGGGAGCTAGCGGAAAATGTCTTTAACCTACCCTTTAAGTTCAAAAAGAAGGTTTACATTTTGGATGAAGCCCACATGCTCACCCCCCAATCTTGGAGTGGGCTTTTAAAGACCTTAGAAGAAGCACCCGATTATGTCTTGTTTATTTTTGCCACCACCGAATTTAACAAGATTCCGATCACGATCTTGTCGCGTTGCCAAAGCTTCTTCTTTAAACAAATTACTAACGATCTCATCCAGCAACGGTTAGCGGAAGTAGCGGCTAAGGAAAGCATTAAGATTACTACTGATGCTTTGGTCAAACTAGCTGATTTAGCGCAAGGTTCGTTGCGTGATGGGTTGAGCTTGTTGGACCAAATTAGTAACTTTAGTGAAAGTAAAACCATTAGTTTAGCTGATGTTGAAAAGACCTTTAACCTGCTTGACAAGGAACAAAAGTTTGGTTTTATTGAAGCAGTTTTAAGTGGTGATCTCAAACAATCCTTTCACTTAATTGACAACTTTGAAAGTCAAGGGATTAACTTTGTTCACTTTCTCAGGGAACTGTTTGCCCTAACAGTTGATTTATATGGTTATGTCAAAACGGGACAAATTGCAGTTGTAAAACCCAGCGACCAAACAATGGCGGCAAAGCTTCGTTTTCACCCCAAACAGTATGCTCTTTTGGTTCAAGCAATTGAAGCTAACAATGGTTATGGTCCTTCACAACTATCCTTAAGTGATCAGATTAAAGCAATTGTGATTCACTATAAAAATGCCGTTAGCAAAGAGCCACACATACCCGCTTATACGCCAGTGGTACAAACTCAATCACCAGCAAAAGAACCCGTTCCTTCAAAAACTGTAGAAGAGGCCAAGCCCCAACTACCAGCAAAAGAACCTGTTCTTTATAAAGTAATAGAAGAACCTAAAGTTTCATCATTCGTAAAAGATCCTGTTGCTTCTAAACTAATAGAACAACCCCAAACTATAGTGCAACCAGAAGCACAGCAAGAAATTACAGAAGTAGAACAACCAACAGAAACTTCACCAGCTCCAGCAACTGATTTATTTGGGTTAGCGATTAAACCAGAGGTGGTACGCAAACGCGGTCGTAGACCTCTTTCTGTAGAAAACACTGATTTTTTTCAGCCCGCTGTTAAAAAATTAATACAGCCTGTAAGCAAGCCTGCTCCTATTAAGTTAATAGAACCATCAGATAATAACCCAGTTTCCATCCCAATAAAACTTAACCGGGCTGTGATTGCCGTTTCTGTTTATGGGCACAATGATCCTAAACTAGTAGCCCACTTCCAACAGTTATTGGACAGCTTTAAAAGGGAGTTCACCCAAGCAGAAAAAACTAAAGACAGCAGTTACTTAAAGCAGTTTTCCGATAAGTTTACCGCTAGTGATTTAAGTAAAGCTATTAAGGTTCTAGCAGCATCACCCTTTGGTTTAGTCTTAATTTTCGAAGATAAAGAGATAGCAACAAGGTTGTGA", "aa": "MRKVLYQKYRPTKFSDTVGQDSIKRIIVNAITQDQLPHGYIFAGERGTGKTTFAKIIAKAINCLNWNGDVCNQCEACQAINSNSAIDVFEIDAASKNGINDIRELAENVFNLPFKFKKKVYILDEAHMLTPQSWSGLLKTLEEAPDYVLFIFATTEFNKIPITILSRCQSFFFKQITNDLIQQRLAEVAAKESIKITTDALVKLADLAQGSLRDGLSLLDQISNFSESKTISLADVEKTFNLLDKEQKFGFIEAVLSGDLKQSFHLIDNFESQGINFVHFLRELFALTVDLYGYVKTGQIAVVKPSDQTMAAKLRFHPKQYALLVQAIEANNGYGPSQLSLSDQIKAIVIHYKNAVSKEPHIPAYTPVVQTQSPAKEPVPSKTVEEAKPQLPAKEPVLYKVIEEPKVSSFVKDPVASKLIEQPQTIVQPEAQQEITEVEQPTETSPAPATDLFGLAIKPEVVRKRGRRPLSVENTDFFQPAVKKLIQPVSKPAPIKLIEPSDNNPVSIPIKLNRAVIAVSVYGHNDPKLVAHFQQLLDSFKREFTQAEKTKDSSYLKQFSDKFTASDLSKAIKVLAASPFGLVLIFEDKEIATRL", "aa_hexdigest": "30a4553e9061dba9d12a36c97762aee3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75177", "query_cov": 1.0, "subject_cov": 0.8737151248164464, "identity": 0.99, "score": 1030.0, "evalue": 0.0, "valid": true, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47658", "cog_id": "COG2812", "cog_category": "L", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47658", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47658" ], "product": "DNA polymerase III subunit gamma/tau" }, "genes": [ "dnaX" ], "id": "GIHJHMOALN_1317", "locus": "GCA009939745_01317" }, { "type": "cds", "sequence": "contig_1", "start": 744395, "stop": 746251, "strand": "-", "frame": 3, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "nt": "TTGTCTTTAATTAACCACTATCAAATTCCCCTAGACCAACCCTTAGGTCAAATGGATACTGATTTAGTACGGATGATCTTGGAGGGTAGTGATGAACCAATTGAGATTAAAACAGTATCAAACTCAGGTGCCAAAAATGTGCGCTTTGAACACTATGAGGGTGTGGCTCACTTAATTAAACGCCGTCACTTAGAGACTAGTAGTCAAGCCAGTCGTGAATGGTACTCGGCTTACATGTCTGAAATTACCTGTAAGAAGTGCCAGGGCAAGAAGCTTACTACTAATTCTTTAAGTGTCAAGTTAGGTGGGTTGGACATTATTAGCTTTACGGAACTCTCGATTGATAAAGCAATTGAATTCTTGTTGCAAATAGAACTGAATCAAGAACAGAAGAAGATTGGTGAGTTAGCTCTTAAAGAGATTATTAACCGTTTATCCTTCTTGAAGAATGTGGGCTTAGAATACCTTAACTTAGCGCGCCGGGCTTCTACCCTGTCAGGTGGTGAAGCACAACGGATTCGCTTAGCCACCCAAATTGGTTCGCAACTCACTGGCGTCCTTTATGTCTTGGATGAACCCTCAATTGGGCTACACCAAAAGGACAATGATCGCTTAATTAACACGATGATGGTAATGCGGGATTTGGGCAACACCTTACTCGTGGTAGAACACGACAGTGAAACCATGCTAGCAGCTGATTACCTCATTGATATTGGTCCTAAAGCGGGTAACCAAGGTGGGGAAGTAGTAGCTGCTGGGACACCGCTAGAGGTAATGGAAAATACTGACTCCTTAACGGGACAGTATCTCAGTGGCAAAAAGCAAATTGAGGTACCAAAAACCCGGCATGCTGGTAATGGTCGCACCTTAACCTTAAAAGGAGCCAAGGGCAACAACTTAAAGAACATTAATGTCACTATTCCCTTAAATAAATTAGTACTAGTGACCGGGGTATCGGGTTCAGGGAAATCAACCTTAATTAACCAAACCTTAGTACCGATCTTGGAACGCTTGGTGAACTATAAGAATGTTAAACCTGCACCCTATAAGGAAATCATAGGGGTCAACCACATTGATAAGGTGGTAGTGGTGTCGCAAGATCCGATCGGTCGCACTCCCCGCTCTAACCCCGCTACCTATGTCAGTGTATTTGATGACATTAGGGAGTTGTTTGCCAACACTAAGGAAGCCAAGGCTCGGGGTTACACTAACTCGCGCTTTTCCTTTAATGTAGCCGGTGGGCGCTGTGATAAGTGTTTTGGTGATGGGGTCATTCGGATTGAAATGCACTTTTTACCCGATGTTTATGTGACGTGTGAAATGTGTGATGGCAAGAAGTACAATCCCCAAACCCTGGAAGTTAAGTACTTAGGCAAATCGATCTTTGATGTGTTACAGATGTCATGTCAGGAAGCTTATGATTTTTTTAAAGCAATTCCGAATATTGCGAGAAAGTTAAAACTACTCTGTGATGTCGGTCTGGAATACCTCCAGTTAGGTCTTAATGTTACCTTCTTGTCAGGTGGGGAAGCACAACGAATTAAACTAGCCAAGTTTCTCCAGAAAAAGGCAACTGGTAAAACCTTGTACGTCCTGGATGAACCTTCAACGGGACTACACATAGAAGACATTAATAAGCTGTTAACGGTGATCCAACGGATTATTAAAAACGGTGATAGTGTCATTATTATTGAGCATAACCTAGATATTATTAAGATGGCGGACTACATCATTGATTTGGGTCCTGAAGGTGGGGAAAAAGGTGGACAAATTATTGCCCAAGGCACACCAGAACAACTGTTAAATCAGGTCGATAAGTCCTACACTGCCCAGTATCTCGCTAAAATCTTAAAGTAA", "aa": "MSLINHYQIPLDQPLGQMDTDLVRMILEGSDEPIEIKTVSNSGAKNVRFEHYEGVAHLIKRRHLETSSQASREWYSAYMSEITCKKCQGKKLTTNSLSVKLGGLDIISFTELSIDKAIEFLLQIELNQEQKKIGELALKEIINRLSFLKNVGLEYLNLARRASTLSGGEAQRIRLATQIGSQLTGVLYVLDEPSIGLHQKDNDRLINTMMVMRDLGNTLLVVEHDSETMLAADYLIDIGPKAGNQGGEVVAAGTPLEVMENTDSLTGQYLSGKKQIEVPKTRHAGNGRTLTLKGAKGNNLKNINVTIPLNKLVLVTGVSGSGKSTLINQTLVPILERLVNYKNVKPAPYKEIIGVNHIDKVVVVSQDPIGRTPRSNPATYVSVFDDIRELFANTKEAKARGYTNSRFSFNVAGGRCDKCFGDGVIRIEMHFLPDVYVTCEMCDGKKYNPQTLEVKYLGKSIFDVLQMSCQEAYDFFKAIPNIARKLKLLCDVGLEYLQLGLNVTFLSGGEAQRIKLAKFLQKKATGKTLYVLDEPSTGLHIEDINKLLTVIQRIIKNGDSVIIIEHNLDIIKMADYIIDLGPEGGEKGGQIIAQGTPEQLLNQVDKSYTAQYLAKILK", "aa_hexdigest": "08c69f143fb9187deb756f3e8ab47b77", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGE6", "query_cov": 0.8187702265372169, "subject_cov": 0.9980276134122288, "identity": 0.706, "score": 716.0, "evalue": 6.19e-254, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0PGE6", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PGE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "product": "UvrABC system protein A" }, "genes": [], "id": "GIHJHMOALN_1318", "locus": "GCA009939745_01318" }, { "type": "cds", "sequence": "contig_1", "start": 746261, "stop": 746611, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGACCGGATTGTCATTAACCAAGACAAGCAAACCTTGGGTCGGATTGTTGATGCGATTGAAGGTGTGATTAAACTAACTGAGGGTCGGATTGAAGTGTTGTTGGAAGATGGTAAGATACTTAGTTTTAATAAGAACCACGGTTGTGATCAGTGTGGTTTTTCAATTAGTGAACTAGAGCCACGCTTATTCTCCTTTAACTCCCCTTTAGGTTCGTGTGAGTACTGTAAGGGTTTAGGTTTTAGTTATGAACCGGATGTGGAAAAGATTATTCCCAATCCGTTGTTGTCCATCAATGAAGGTGGTATTGACATCTTTAAGAACATAGTGCACGGTACATCACTAGATTGA", "aa": "MDRIVINQDKQTLGRIVDAIEGVIKLTEGRIEVLLEDGKILSFNKNHGCDQCGFSISELEPRLFSFNSPLGSCEYCKGLGFSYEPDVEKIIPNPLLSINEGGIDIFKNIVHGTSLD", "aa_hexdigest": "c864bc54e6c03a8afa32be3527d46d3a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12827.505099999984, "isoelectric_point": 4.7428388595581055 }, "id": "GIHJHMOALN_1319", "locus": "GCA009939745_01319" }, { "type": "cds", "sequence": "contig_1", "start": 746885, "stop": 747241, "strand": "-", "frame": 3, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0G0SCP6", "UniRef:UniRef90_A0A0G0SCP6" ], "nt": "TTGAAACCAGAGCTAAAAACTAATGATTTTATTCGCGTTAAGGGCGCGCGTGAAAACAACCTGAAGAATGTAAACATCGATATTCCCAAAAACCAGTTTGTCGTTATTACGGGGTTGTCGGGTTCGGGTAAATCATCACTTGCCTTTAACACCATTTACGCGGAAGGCCGCCGTCGGTATTTGGAATCACTCTCGTCATATGCCAGACAGTTTCTCGGTAACAGTGATAAACCGGATGTAGATTCGATTGAAGGGTTATCCCCAGCCATTTCAATTGACCAAAAAACCACTTCACACAATCCCCGCTCCACTGTGGGAACAGTAACGGAAATTTACGATTACCTCCGTTTACTGTGA", "aa": "MKPELKTNDFIRVKGARENNLKNVNIDIPKNQFVVITGLSGSGKSSLAFNTIYAEGRRRYLESLSSYARQFLGNSDKPDVDSIEGLSPAISIDQKTTSHNPRSTVGTVTEIYDYLRLL", "aa_hexdigest": "3e2ffd1f63e310734d00ad5da4e8ac46", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_X0QG93", "query_cov": 0.940677966101695, "subject_cov": 0.8809523809523809, "identity": 0.82, "score": 178.0, "evalue": 9.2e-56, "valid": false, "product": "UvrABC system protein A", "uniref50_id": "UniRef50_X0QG93" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 746582, "stop": 747214, "observations": { "mutation": [ 746887 ], "stop": [ 746887 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A0G0SCP6", "query_cov": 0.923728813559322, "subject_cov": 0.40977443609022557, "identity": 0.835, "score": 186.0, "evalue": 6.48e-57, "gene_start": 10, "gene_end": 118, "reference_start": 4, "reference_end": 112, "reference_sequence": "MEQFIKIRGARQHNLKNVDLDIPKNKLVVLTGVSGSGKSSLAFDTLYAEGQRRYVESLSSYARQFLGIMEKPDVDSIEGLSPAISIDQKTTSHNPRSTVGTVTEIYDYLRLLFARIGHPHCPVCGREISHQTLDQIVDQTMNLIETTAKADKKAWFLVLSPMVVARKGEFSQLFENLKSKGYRQVRIDGFIHDLSEDLILIKTNKHTIEAVTDRVALSADSLKGQPSKAELKNRLADSIQQALNLSEGMLVLAHVQDKEFTQYQSS" }, "paralog": false, "identity": 0.5806451612903226, "score": 247.0, "evalue": 2.39e-83, "description": "Internal stop codon at 746887. Nonsense mutation around 746887.", "psc": { "uniref90_id": "UniRef90_A0A0G0SCP6", "product": "UvrABC system protein A", "uniref50_id": "UniRef50_A0A0G0SCP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A0G0SCP6", "UniRef:UniRef50_A0A0G0SCP6" ] } }, "id": "GIHJHMOALN_1320", "locus": "GCA009939745_01320" }, { "type": "cds", "sequence": "contig_1", "start": 747219, "stop": 747482, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTAGCGATTTAAATAAAATTAACACCAAGCAACGCTTTAAACTTTACCTGTTATTTAAGCTATTGTTACACCCCCAGTTGGTGTTGGTCGACTCCTTTGTCAACTTTAACAAACACACTTATAACTTTACCCGTGGTTTGTTAATTGCCCACCAAAACCAACAAGGCATTGCATATTTATTTAACGATCCGCACAATAACCTCGTAAAGGATTTTTTCACCCAAACAATTAACTTTGAAACCAGAGCTAAAAACTAA", "aa": "MIFSDLNKINTKQRFKLYLLFKLLLHPQLVLVDSFVNFNKHTYNFTRGLLIAHQNQQGIAYLFNDPHNNLVKDFFTQTINFETRAKN", "aa_hexdigest": "adb64aeb952abc6ab0434b9db13fe4f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10373.862199999989, "isoelectric_point": 9.820742988586424 }, "id": "GIHJHMOALN_1321", "locus": "GCA009939745_01321" }, { "type": "cds", "sequence": "contig_1", "start": 747483, "stop": 747803, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTACAAAAAAGAAATTAAGCAAAAGCAACAGCAAATTAAAGCACTGCTAAAAAATTATGGCACTTTAAATAAGCTGCTCAATACTCAAATTAGTAAAGTCAATAACGTGGTACGCAAAACCTTCTTTGTGGATTCAGAAGAGTGTGATTTAAACCGTTTACAGGCATCGAATAAGCTGCACTTTAATCTCTTAAACGCGATGGTGAATGTCATTAGTTTTTGTCTCAAAAAGTGCCGGCAAAATCCCAAAAAACTAAACCGTACTGCTAACCTCAAAATGCTGTTGGATAACACCTTTAAAAACGGTATTCCGAGCTGA", "aa": "MYKKEIKQKQQQIKALLKNYGTLNKLLNTQISKVNNVVRKTFFVDSEECDLNRLQASNKLHFNLLNAMVNVISFCLKKCRQNPKKLNRTANLKMLLDNTFKNGIPS", "aa_hexdigest": "405d74d65708e6fa8d928a0ccd470747", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12328.422499999982, "isoelectric_point": 10.112978172302242 }, "id": "GIHJHMOALN_1322", "locus": "GCA009939745_01322" }, { "type": "cds", "sequence": "contig_1", "start": 747852, "stop": 748001, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAAAACAGTTAATAAATACTTCGAAGCCAACTTGCACCACCCTCAAGCTTACTTGTTGTTACCTAATCGTAATGCCACTACTTTAACAGAAGCAAACACCACTACCATAAACCAACTTAACTTAAAAACCCAGCTCAAACAGTGA", "aa": "MDKTVNKYFEANLHHPQAYLLLPNRNATTLTEANTTTINQLNLKTQLKQ", "aa_hexdigest": "1b3e1ae7f1d1d2e8b3a0e3be866d8aef", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5655.358000000001, "isoelectric_point": 9.303061866760252 }, "id": "GIHJHMOALN_1323", "locus": "GCA009939745_01323" }, { "type": "cds", "sequence": "contig_1", "start": 748026, "stop": 748157, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACAAACAAACACAACACTTCTTTACCAAATACCAGTTTATTACCCATGGTGTGGTTGACTTTGTCTTTAACCAAGGTCGGAATCAAACTCAGTTTGCCAGCTTAAAGGCCAACTTAAACCGCGATTGA", "aa": "MHKQTQHFFTKYQFITHGVVDFVFNQGRNQTQFASLKANLNRD", "aa_hexdigest": "26159408fa34c49b1761e9617fcb62e9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5143.711000000001, "isoelectric_point": 9.992099952697757 }, "id": "GIHJHMOALN_1324", "locus": "GCA009939745_01324" }, { "type": "cds", "sequence": "contig_1", "start": 748212, "stop": 748574, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAGTTAACTAAGCCAATTGTTTTGACTCATGATGCGGTGATTGGTTTTTTAATTAAATCTCGTTTAGCTTTTTTGCAAGAATACGCTAAGGGCTTAAATCGCTGTGAGCAACACAACAAGCTGGTCAATGAGTTAAAGCAAAACGTTCTGTTAAACCAAAACCAGTATAAGGGTGAGGTTAGCCAAAGCTATTCGGTTGTAAACCAAAAGGCGGTTTTTAAGGGTTTTATCCAAACAGTTAAAGCAGCGCTTAACTACACTAAGTTAAAACACACCTTAGATCCGTTTAACCTAATGAATATTGTTCAAGAACGCTGTTTTGAACAACTCTTAACTACTTACGAAAAATTAGATTGA", "aa": "MEKLTKPIVLTHDAVIGFLIKSRLAFLQEYAKGLNRCEQHNKLVNELKQNVLLNQNQYKGEVSQSYSVVNQKAVFKGFIQTVKAALNYTKLKHTLDPFNLMNIVQERCFEQLLTTYEKLD", "aa_hexdigest": "9084ed01dc385e38f2f74d0a8d14c4ac", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13918.025699999986, "isoelectric_point": 9.250649070739744 }, "id": "GIHJHMOALN_1325", "locus": "GCA009939745_01325" }, { "type": "cds", "sequence": "contig_1", "start": 748599, "stop": 748733, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCTTACTTAATCCCGATTTGTTAGTGAGCACAATTGCTGAAAGTGAGCGCCTAGTAGAACAGTTAATGAGCAACATCCAGCGCTTGTACCAGCAAATTTTGCAGCTAAAACCAGGTCAATCACTTAACTGA", "aa": "MRLLNPDLLVSTIAESERLVEQLMSNIQRLYQQILQLKPGQSLN", "aa_hexdigest": "561e64c6286c3fb9f7101f765077ed75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5094.9057999999995, "isoelectric_point": 6.014099693298339 }, "id": "GIHJHMOALN_1326", "locus": "GCA009939745_01326" }, { "type": "cds", "sequence": "contig_1", "start": 748755, "stop": 749324, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCTTTAATGCACTCAAGAAGTTAAGCCAGTTTGTGGTGTTTGAACTAGATAACACCAATAAGTTAACGAGTGAAACACTTAGTCAGTTCTTCACCACTAAGTTTGTCAATAAGGTGTACTTTAACCAGATCCAGTTTGAGTACAACAACTTTGTTGGTTTATTGGAAGACCATGAACGTGACCTAGTGGAATTTAGCACCCACTTCTTTAACAGCTTCTTTGAACAACCTGATCACTTAACTAAAAGTTTTTTTGAACAGTACTATGCGCTTACAAGACAACGAGAACGTTTACTGCACAATTTAAAAGTTCAAAGCTATGAAACCAAACACGGGGTTAATACCTACTTTGAAAATTTGCAAATTACCCGTGCACAACAGCAAGCCAATCAGCTTAAGCGTGCCTTCAAAAAACAGCAGCGCAATTCGGTGCAGCTGATTAACCGCTTTATGTCGTCCTTGTGGTGGAGTCAAACCAAGATTAAGTTCAAGAGTATCTTTAACTTTTACAAAACCCGGATTTTGGAAAAACGGATTGTAGCCAAGCGAATCCAAATCAAAATTTGA", "aa": "MGFNALKKLSQFVVFELDNTNKLTSETLSQFFTTKFVNKVYFNQIQFEYNNFVGLLEDHERDLVEFSTHFFNSFFEQPDHLTKSFFEQYYALTRQRERLLHNLKVQSYETKHGVNTYFENLQITRAQQQANQLKRAFKKQQRNSVQLINRFMSSLWWSQTKIKFKSIFNFYKTRILEKRIVAKRIQIKI", "aa_hexdigest": "eadd1df54e193b03ba5c58100650fccf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22985.023500000014, "isoelectric_point": 9.958447456359863 }, "id": "GIHJHMOALN_1327", "locus": "GCA009939745_01327" }, { "type": "cds", "sequence": "contig_1", "start": 749784, "stop": 751469, "strand": "-", "frame": 2, "gene": "rnjA", "product": "putative MG423-like protein", "db_xrefs": [ "COG:COG0595", "COG:J", "GO:0003723", "GO:0005886", "GO:0046872", "RefSeq:WP_010874978.1", "SO:0001217", "UniParc:UPI00001397B4", "UniRef:UniRef100_P75174", "UniRef:UniRef50_P47662", "UniRef:UniRef90_P75174" ], "nt": "ATGGCTAAAATTAACTTCTTTGCCTTCGGTGGTCAGGACGAGAGGGGCAAGAATTGTTTTGTTCTCGAAATCAATAACGATGTTTTTATTTTTAACGTTGGTAGTTTGACACCAACAACCGCTGTTTTAGGGGTCAAAAAGATCATCCCTGACTTTAGTTGGATCCAGGAAAACCAGGCCCGCATTAAGGGCATCTTTATCGGTAATCCAGTAACGGAAAACATTGGTTCGCTGGAGTTCTTATTCCACACCGTTGGTTTCTTTCCGATTTACACCAGTACGATTGGCGCTGTGGTTATTAAAACTAAGATCCACGAAAACAAGCTCAATATTCCCCATGACGAGTTGGAAATCCACGAGCTTAAACCGTTAGAAACGGTCAAGATCGGGCACCATAACATTACGCCCTTTAAGGTATCGAGTTCGATTCCTTCCTCGTTTGGCTTTGCGCTCCACACTGATGATGGGTACATTGTCTATGTCGATGACTTCATTGTGCTCAATGATAAGAACATCGCCTTTGAAAACCAACTCAATCAGATTATTCCCCAAGTAGCGAACAAGACCCTTTTGTTAATTACTGGAGTTGGTCTAGTGGGACGTAACACTGGTTTTACAACCCCTAAGCACAAATCCTTGGAACAACTCAACCGCATTATTGCCTCCGCTAAGGGTCGGGTATTTGCTGCTTGTTATGATTCCAACGCTTACAGTGTGATGACCTTGGCCCAAATTGCACGGATGCAAAACCGCCCCTTTGTCATCTACTCCCATTCATTTGTCCACCTCTTCAACGCGATTGTGCGCCAAAAGCTGTTTAACAACACCCACTTAAACACTATTTCGATTGAAGAGATTAACAACTCCACAAACGCCATTGTTGTTTTAACCGCGCCACCAGATAAGTTGTATGCCAAGCTGTTTAAGATTGGTACTAATGAGGATGAACGGGTACGGTATCGCAAGACCGATAGCTTTATCTTCATGATTCCCCGCATAGCTGGGTATGAAGAGTTGGAAGCACAAATCTTGGACGATGTCGCGCGTAATGAGGTGAGTTACTACAACTTGGGTCGGGAAATCTTGTCGATCAATGCATCGGATGAAGACATGAAGTTTTTAGTAACTTCCTTAAAACCTAAGTACATTATTCCCACATCCGGTCTGTACCGTGACTTCATTAACTTTACCATGGTAATGAAGCAAGCTGGTGTGGAACAAAGTCAGGTCTTAATTCCCTTTAATGGTGAAGTCCTAGCCATTAACCACAAGCAAATTGACAACAAAAAACGGGAATTAAAGCTCAACCCTAAGTGTGTTGACTCCGCTGGTTTGCAGGAAATAGGGGCCAGCATCATGTTTGAGCGTGATCAGATGTCTGAAGCGGGTGTGGTTACCATCATCATCTACTATGACTCCAAGAAGAGTGAGTTCCTAAACGAAATTACCTATTCCTTTTTAGGAGTTAGCCTAGATAGTAACAATCAGGTTAAACTCAAGACCAAAATGGAAGAGTTAATTCGCAAACAAATTAACGACATTAAGGACTTTACGACAATTAAGCGACGTTTAGGCAAAGACACATCGAAGGAACTCAAGGTTTCTATTAAACGAGCTGTCATGAATTTATTTACCAAAATGACTGCTAAAGCACCACTAATCCTGTCAACTATTATTTCTATTTAG", "aa": "MAKINFFAFGGQDERGKNCFVLEINNDVFIFNVGSLTPTTAVLGVKKIIPDFSWIQENQARIKGIFIGNPVTENIGSLEFLFHTVGFFPIYTSTIGAVVIKTKIHENKLNIPHDELEIHELKPLETVKIGHHNITPFKVSSSIPSSFGFALHTDDGYIVYVDDFIVLNDKNIAFENQLNQIIPQVANKTLLLITGVGLVGRNTGFTTPKHKSLEQLNRIIASAKGRVFAACYDSNAYSVMTLAQIARMQNRPFVIYSHSFVHLFNAIVRQKLFNNTHLNTISIEEINNSTNAIVVLTAPPDKLYAKLFKIGTNEDERVRYRKTDSFIFMIPRIAGYEELEAQILDDVARNEVSYYNLGREILSINASDEDMKFLVTSLKPKYIIPTSGLYRDFINFTMVMKQAGVEQSQVLIPFNGEVLAINHKQIDNKKRELKLNPKCVDSAGLQEIGASIMFERDQMSEAGVVTIIIYYDSKKSEFLNEITYSFLGVSLDSNNQVKLKTKMEELIRKQINDIKDFTTIKRRLGKDTSKELKVSIKRAVMNLFTKMTAKAPLILSTIISI", "aa_hexdigest": "d8eed88c141103209b004c9f0e6aed2d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001397B4", "ncbi_nrp_id": "WP_010874978.1", "uniref100_id": "UniRef100_P75174", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001397B4", "RefSeq:WP_010874978.1", "UniRef:UniRef100_P75174" ] }, "ips": { "uniref100_id": "UniRef100_P75174", "uniref90_id": "UniRef90_P75174" }, "psc": { "uniref90_id": "UniRef90_P75174", "gene": "RnjA", "product": "Uncharacterized protein MG423 homolog", "uniref50_id": "UniRef50_P47662", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0046872", "GO:0003723" ] }, "pscc": { "uniref50_id": "UniRef50_P47662", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47662" ], "product": "Uncharacterized protein MG423" }, "genes": [ "rnjA" ], "id": "GIHJHMOALN_1328", "locus": "GCA009939745_01328" }, { "type": "cds", "sequence": "contig_1", "start": 751474, "stop": 751734, "strand": "-", "frame": 1, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_010874979.1", "SO:0001217", "UniParc:UPI0000134D13", "UniRef:UniRef100_P75173", "UniRef:UniRef50_P47663", "UniRef:UniRef90_P75173" ], "nt": "ATGCAAATTGATAAAAACGGGATCATTAAATCCGCACAACTCCACGACAAGGACGTGGGTAGTATTCAGGTACAAGTGTCCCTTTTAACGAGCCAAATTAAACAGCTCACCGACCACTTACTAGCTAACAAGAAGGACTTTATTTCCAAACGTGGTTTGTACGCGAAAGTATCAAAGCGCAAGCGCTTGTTGAAGTACTTAAAGCACAACGATTTAGAGGCTTACAGAAATTTAGTTAAAACCTTAAATCTCCGGGGTTAG", "aa": "MQIDKNGIIKSAQLHDKDVGSIQVQVSLLTSQIKQLTDHLLANKKDFISKRGLYAKVSKRKRLLKYLKHNDLEAYRNLVKTLNLRG", "aa_hexdigest": "e53f2d5f814b7b149311b178fd0d2b9b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134D13", "ncbi_nrp_id": "WP_010874979.1", "uniref100_id": "UniRef100_P75173", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134D13", "RefSeq:WP_010874979.1", "UniRef:UniRef100_P75173" ] }, "ips": { "uniref100_id": "UniRef100_P75173", "uniref90_id": "UniRef90_P75173" }, "psc": { "uniref90_id": "UniRef90_P75173", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P47663", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47663" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "GIHJHMOALN_1329", "locus": "GCA009939745_01329" }, { "type": "cds", "sequence": "contig_1", "start": 751892, "stop": 752233, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35" ], "nt": "ATGGACTCAACTTTTAATGAACTAGGCGTCTCTCCTGCCTTAATTGCCACACTCAAAGATAACAACATTAACCAACCAACTACGATTCAACAACTGGCTATTCCCCAGTTTCTCCAACATCAAAACCTGATTGTCCACTCCCCCACCGGCACTGGTAAAACCGCTGTTTTTGCCATTCCGGTCATTGAAACACTCCTTAAAAAACCAAGTAAAGGTACGACCCAAACATTGGTAGTTGCACCTACCCGTGAACTGGCCGAACAGATTAAAACCACCTTTATTAACTTTGCTAAACACACCCATTTAAAGGTAGTGAGCTTAATTGGTGGCATCCCCATTTGA", "aa": "MDSTFNELGVSPALIATLKDNNINQPTTIQQLAIPQFLQHQNLIVHSPTGTGKTAVFAIPVIETLLKKPSKGTTQTLVVAPTRELAEQIKTTFINFAKHTHLKVVSLIGGIPI", "aa_hexdigest": "6f28b63d51c4fb90203bf0d1d38e6b1a", "start_type": "ATG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 84, "aa_cov": 0.7433628318584071, "hmm_cov": 0.9761904761904762, "evalue": 4.864591825668513e-25, "score": 83.68274688720703, "start": 27, "stop": 110 } ], "seq_stats": { "molecular_weight": 12220.109599999982, "isoelectric_point": 9.523156929016114 }, "id": "GIHJHMOALN_1330", "locus": "GCA009939745_01330" }, { "type": "cds", "sequence": "contig_1", "start": 752282, "stop": 753229, "strand": "+", "frame": 2, "gene": "srmB", "product": "putative ATP-dependent RNA helicase MG425-like protein", "db_xrefs": [ "COG:COG0513", "COG:L", "EC:3.6.4.13", "GO:0003689", "GO:0003723", "GO:0003724", "GO:0005524", "GO:0005829", "GO:0016887", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "SO:0001217", "UniRef:UniRef50_P47664", "UniRef:UniRef90_P75172" ], "nt": "ATGGGGCGGGTGATGGACCTGTTAGAACGGGGTGTAATTAAGTTTGAACACCTAGAACACCTCATTATTGACGAGGTTGACCTAATGCTCGACCGTGGTTTTAAGAGAAAACTGTTTGATCTGTTAGGTCGCATTGAAAAGTTTGAACAAATTGCGGTTTATTCTGCTAGTTACAATGAAGAGACAATTGAAACAGCTAAACAAATTACCAAGAATGGTATTTTCCTAGCAGCACCAGAATTAAAACAAAATGCCCCTGAACCAGATAACAAACTCATTGATCAATTTGTTTGTTATCTTTTTTCTAACCGGAAGAAACAAGCCCTCTATTCTTTGGTATCGCAAACTAGAGCGAAGTCGATTATTGTTTTTTGTGACACCAAGAAGTTAGTTGATGAGCTGTGCATCTTCTTGCGCAAGAATGATGTCAAAACTTATCCACTTCACGGGGACAAAGCGCAGTTCATTCGCGAACGCAACTTAAAGCTGTTTGCTAACACAACTGCTCCTATAGTATTAGTAACAACTGATCTAATCGGTCGTGGTATCCATGTGGAAGGGGTGGACATGGTAGTCAACTATTCCGCTTGTGTAAACTTTGAAACTTATTTGCACCGGATGGGTCGCACAGGACGGAACAACCACAAGGGCTCATGTATTACCTTCTGTACCTCACACGAAAAGCAAGCATTTTTAAAGTTATTAGAACAAGTTAACGACAAGCGCATTAGCCCTCTCCGCCCCATGCGCTTAAGGTTAATTCCCTTGAAGTGCAAAACCCAACCCAAGAAGGGGAAACTTTCCTTACAGTCAGTGCAAAAGATTTACGTCAATCCTCGTTCCAATGGCACCTTTAAACGCGTACCTTTAGCGGGGGATTTGTTTAAAAGCCGGATGCGTCAACCTGAGCGGGACATGCAAAAGAACAAATTGCATGACAGTGATTGA", "aa": "MGRVMDLLERGVIKFEHLEHLIIDEVDLMLDRGFKRKLFDLLGRIEKFEQIAVYSASYNEETIETAKQITKNGIFLAAPELKQNAPEPDNKLIDQFVCYLFSNRKKQALYSLVSQTRAKSIIVFCDTKKLVDELCIFLRKNDVKTYPLHGDKAQFIRERNLKLFANTTAPIVLVTTDLIGRGIHVEGVDMVVNYSACVNFETYLHRMGRTGRNNHKGSCITFCTSHEKQAFLKLLEQVNDKRISPLRPMRLRLIPLKCKTQPKKGKLSLQSVQKIYVNPRSNGTFKRVPLAGDLFKSRMRQPERDMQKNKLHDSD", "aa_hexdigest": "eae0cc757cc6827ce282a203a09dbc70", "start_type": "ATG", "rbs_motif": null, "genes": [ "srmB" ], "truncated": "both", "pseudogene": { "start": 751982, "stop": 753244, "observations": { "mutation": [ 752231, 753227 ], "stop": [ 752231, 753227 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75172", "query_cov": 1.0, "subject_cov": 0.7, "identity": 0.997, "score": 624.0, "evalue": 9.71e-224, "gene_start": 1, "gene_end": 315, "reference_start": 131, "reference_end": 445, "reference_sequence": "MDSTFNELGVSPALIATLKDNNINQPTTIQQLAIPQFLQHQNLIVHSPTGTGKTAVFGIPVIETLLKKPSKGTTQTLVVAPTRELAEQIKTTFINFAKHTHLKVVSLIGGIPIWQQLKQLENQPEIVVGTMGRVMDLLERGVIKFEHLEHLIIDEVDLMLDRGFKRKLFDLLSRIEKFEQIAVYSASYNEETIETAKQITKNGIFLAAPELKQNAPEPDNKLIDQFVCYLFSNRKKQALYSLVSQTRAKSIIVFCDTKKLVDELCIFLRKNDVKTYPLHGDKAQFIRERNLKLFANTTAPIVLVTTDLIGRGIHVEGVDMVVNYSACVNFETYLHRMGRTGRNNHKGSCITFCTSHEKQAFLKLLEQVNDKRISPLRPMRLRLIPLKCKTQPKKGKLSLQSVQKIYVNPRSNGTFKRVPLAGDLFKSRMRQPERDMQKNKLHDSDWQSNM" }, "paralog": false, "identity": 0.9904761904761905, "score": 822.0, "evalue": 2.69e-304, "description": "Internal stop codon at 752231, 753227. Nonsense mutation around 752231, 753227.", "psc": { "uniref90_id": "UniRef90_P75172", "gene": "SrmB", "product": "Probable ATP-dependent RNA helicase MG425 homolog", "ec_ids": [ "3.6.4.13" ], "uniref50_id": "UniRef50_P47664", "cog_id": "COG0513", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003723", "GO:0003724" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75172", "EC:3.6.4.13", "UniRef:UniRef50_P47664", "COG:COG0513", "COG:L", "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003723", "GO:0003724" ] }, "pscc": { "uniref50_id": "UniRef50_P47664", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47664" ], "product": "Probable ATP-dependent RNA helicase MG425" } }, "id": "GIHJHMOALN_1331", "locus": "GCA009939745_01331" }, { "type": "cds", "sequence": "contig_1", "start": 753248, "stop": 753463, "strand": "+", "frame": 2, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "COG:COG0227", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "SO:0001217", "UniRef:UniRef50_Q6MTC5", "UniRef:UniRef90_P75171" ], "nt": "TTGTTACAATTATTTGATATGGCAAAAAAAGACCAACTTACTTTAAGAGGGCCTTTGTATGGCAACAATCGTTCCCACTCCAAGACTATTACGCGCAGAAAGTGGAATGTTAACCTCCAACCATGCAAGGTCAAAACTGCTGATGGCAAGACCACCAGAATCTTAGTTTCTACCAGAACACTGCGCACCTTAAAGAAACACAACCGCCTCTCTTAG", "aa": "MLQLFDMAKKDQLTLRGPLYGNNRSHSKTITRRKWNVNLQPCKVKTADGKTTRILVSTRTLRTLKKHNRLS", "aa_hexdigest": "74510e73d215b5087aac105bb3dc7cc6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75171", "query_cov": 0.9154929577464789, "subject_cov": 1.0, "identity": 1.0, "score": 127.0, "evalue": 4.1e-37, "valid": true, "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q6MTC5", "cog_id": "COG0227", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MTC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MTC5" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "GIHJHMOALN_1332", "locus": "GCA009939745_01332" }, { "type": "cds", "sequence": "contig_1", "start": 753507, "stop": 753932, "strand": "+", "frame": 3, "gene": "yhfA", "product": "Hydroperoxide reductase", "db_xrefs": [ "COG:COG1765", "COG:R", "EC:1.11.1.-", "GO:0004601", "GO:0005737", "RefSeq:WP_010874982.1", "SO:0001217", "UniParc:UPI00001397C2", "UniRef:UniRef100_P75170", "UniRef:UniRef50_P47666", "UniRef:UniRef90_P75170" ], "nt": "ATGGACAAAAAGTACGACATCACTGCTGTTTTAAACGAAGACAGCTCCATGACTGCCATCAGTGATCAGTTTCAAATTACACTCGATGCCCGTCCCAAGCACACCGCTAAGGGTTTTGGTCCTTTAGCTGCTCTGCTCTCTGGTTTAGCTGCTTGTGAATTGGCAACCGCTAACTTGATGGCACCAGCTAAAATGATTACCATCAATAAACTGTTGATGAATGTCACTGGTTCACGCTCAACCAACCCCACCGATGGTTATTTTGGTTTAAGGGAAATTAACCTGCACTGGGAAATCCACTCCCCTAACAGTGAAACGGAAATCAAGGAGTTCATTGACTTTGTTTCCAAACGCTGCCCAGCGCACAATACGCTCCAAGGTGTGAGTCAACTCAAGATTAACGTTAATGTAACTTTAGTGCATTAA", "aa": "MDKKYDITAVLNEDSSMTAISDQFQITLDARPKHTAKGFGPLAALLSGLAACELATANLMAPAKMITINKLLMNVTGSRSTNPTDGYFGLREINLHWEIHSPNSETEIKEFIDFVSKRCPAHNTLQGVSQLKINVNVTLVH", "aa_hexdigest": "72cd36b4ae77fb22d150dbc14598aff0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001397C2", "ncbi_nrp_id": "WP_010874982.1", "uniref100_id": "UniRef100_P75170", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001397C2", "RefSeq:WP_010874982.1", "UniRef:UniRef100_P75170" ] }, "ips": { "uniref100_id": "UniRef100_P75170", "uniref90_id": "UniRef90_P75170" }, "psc": { "uniref90_id": "UniRef90_P75170", "gene": "YhfA", "product": "Hydroperoxide reductase", "ec_ids": [ "1.11.1.-" ], "uniref50_id": "UniRef50_P47666", "cog_id": "COG1765", "cog_category": "R", "go_ids": [ "GO:0005737", "GO:0004601" ] }, "pscc": { "uniref50_id": "UniRef50_P47666", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47666" ], "product": "Hydroperoxide reductase" }, "genes": [ "yhfA" ], "id": "GIHJHMOALN_1333", "locus": "GCA009939745_01333" }, { "type": "cds", "sequence": "contig_1", "start": 754163, "stop": 754309, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAATTGCGCTCCACGCCGAGCATGACTCCAAGAAGCGCTATGACCCCAAATACGGTTTGAGCTTTGAGACCTACTTGAAGTTAAATGGTGCTAACTTCATTAAGTCGAGTTACCGTAGCTTAATGAATAAGGTTGATGACTGA", "aa": "MSIALHAEHDSKKRYDPKYGLSFETYLKLNGANFIKSSYRSLMNKVDD", "aa_hexdigest": "27e5f115f6e63c225c5d5c1df04044e8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5583.270300000002, "isoelectric_point": 9.049830055236818 }, "id": "GIHJHMOALN_1334", "locus": "GCA009939745_01334" }, { "type": "cds", "sequence": "contig_1", "start": 754325, "stop": 754585, "strand": "+", "frame": 2, "gene": null, "product": "putative MG428-like protein", "db_xrefs": [ "GO:0003700", "GO:0006352", "SO:0001217", "UniRef:UniRef50_P47667", "UniRef:UniRef90_P75169" ], "nt": "ATGAAAACACTCGAGGAACAAAACACAGTCTCTTACACTCCTGAAAACTATTTAAGAAGTGTAGAATTCCGTGAAATGGTGCACTTAGCGTTTAAGAAAGCCAAGAATGAGGATGAACGTCAGGTGTTTGCGCTTTATGTAAAGGGTTACAAAAATTTAGAGATTGCTAAAAAACTCAACCTAACGACCCGTAGAGTGCGCTACCTAATTTCAATCTTTAAGGATCACATTAAGGTATTGACTAAACGGTATGGTTACTAG", "aa": "MKTLEEQNTVSYTPENYLRSVEFREMVHLAFKKAKNEDERQVFALYVKGYKNLEIAKKLNLTTRRVRYLISIFKDHIKVLTKRYGY", "aa_hexdigest": "239f6dca832476888c6b6e2744552adc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 754067, "stop": 754585, "observations": { "mutation": [ 754121, 754307 ], "stop": [ 754121, 754307 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75169", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 168.0, "evalue": 6.81e-52, "gene_start": 1, "gene_end": 86, "reference_start": 87, "reference_end": 172, "reference_sequence": "MKLQIFNTKLGLLAAKIYWKSWRHLGLSEDEIVSIALHAEHDSKKRYDPKYGLSFETYLKLNGANFIKSSYRSLMNKVDDWIGFKDMKTLEEQNTVSYTPENYLRSVEFREMVHLAFKKAKNEDERQVFALYVKGYKNLEIAKKLNLTTRRVRYLISIFKDHIKVLTKRYGY" }, "paralog": false, "identity": 0.9825581395348837, "score": 334.0, "evalue": 1.12e-119, "description": "Internal stop codon at 754121, 754307. Nonsense mutation around 754121, 754307.", "psc": { "uniref90_id": "UniRef90_P75169", "product": "Uncharacterized protein MG428 homolog", "uniref50_id": "UniRef50_P47667", "go_ids": [ "GO:0003700", "GO:0006352" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75169", "UniRef:UniRef50_P47667", "GO:0003700", "GO:0006352" ] }, "pscc": { "uniref50_id": "UniRef50_P47667", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47667" ], "product": "Uncharacterized protein MG428" } }, "id": "GIHJHMOALN_1335", "locus": "GCA009939745_01335" }, { "type": "cds", "sequence": "contig_1", "start": 754582, "stop": 754812, "strand": "-", "frame": 1, "gene": null, "product": "PEP-utilising enzyme C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0K9GSR8" ], "nt": "ATGTGTGGTGAGATGGCCGGTGAACCGTTAGCGATTCCCCTACTTTTAGGTTTGGGTCTCAAAGAGTTTTCCATGAGTGCTAGTTCCATGTTTAAGGCGCGGATGATTATCGCTAAGCTCAATTACACAGAGTGTCAGACATTAGCACAAAAAGCATTAACCTTAGCGAACGCCAAGGAGGTGGAAAAGCTAGTGGAAAAATTCTTCAAGAAGAAGGATATCTTCATCTAG", "aa": "MCGEMAGEPLAIPLLLGLGLKEFSMSASSMFKARMIIAKLNYTECQTLAQKALTLANAKEVEKLVEKFFKKKDIFI", "aa_hexdigest": "34a06d14b9bb79439c625a730ce9b8d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A6L3V2M9", "query_cov": 0.8552631578947368, "subject_cov": 0.9027777777777778, "identity": 0.569, "score": 69.3, "evalue": 4.51e-14, "valid": false, "product": "PEP-utilising enzyme C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A0K9GSR8" }, "pscc": { "uniref50_id": "UniRef50_A0A0K9GSR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0K9GSR8" ], "product": "PEP-utilising enzyme C-terminal domain-containing protein" }, "genes": [], "id": "GIHJHMOALN_1336", "locus": "GCA009939745_01336" }, { "type": "cds", "sequence": "contig_1", "start": 754819, "stop": 755340, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "GTGTTAGAACAAGCGCACGGTGATTTAGTGATTATCCGCACCCTAGACATTGGTGGGGACAAGAAGCTTAATTACTATGAGTTTCCTCACGAAGATAACCCCTTCTTGGGCTACCGCGCTTTGCGTTTAACCTTAGACAAACAGGACATCTTTAAAACACAATTGCGGGCTTTACTACGTGCTGCTGATCACGGTCAGTTGGGCATTATGTTCCCGATGGTCGCTACCTTGGATGAACTGTTGCAAGCCAAGCAACTGTTAAACCAGGTGCACCAGGAGTTGGGTGGCAATAAACAGTTTAAGCTCGGGATTATGATCGAAATCCCCGCTGCTGTTTTAGCGGCTAACACCTTAAGTCACCACGTGGATTTCTTCTCCATTGGTACCAATGACCTGATCCAGTACAGCTTTGCGGCTGACCGGATGAATAAGAATGTTAGTTATCTCTACCAACCTTTAAACCCAGCTTTATTAAAACTCATTTACCTCACCATTGAAGGGGGTAAAGTGAATGATATTTGA", "aa": "MLEQAHGDLVIIRTLDIGGDKKLNYYEFPHEDNPFLGYRALRLTLDKQDIFKTQLRALLRAADHGQLGIMFPMVATLDELLQAKQLLNQVHQELGGNKQFKLGIMIEIPAAVLAANTLSHHVDFFSIGTNDLIQYSFAADRMNKNVSYLYQPLNPALLKLIYLTIEGGKVNDI", "aa_hexdigest": "0c7ee1613f3aa3f4bc83d4d45e76f441", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 174, "aa_cov": 0.976878612716763, "hmm_cov": 1.0, "evalue": 2.721245758839261e-71, "score": 235.5618133544922, "start": 1, "stop": 169 } ], "seq_stats": { "molecular_weight": 19522.377600000003, "isoelectric_point": 6.038313102722167 }, "id": "GIHJHMOALN_1337", "locus": "GCA009939745_01337" }, { "type": "cds", "sequence": "contig_1", "start": 755377, "stop": 756300, "strand": "-", "frame": 1, "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "db_xrefs": [ "COG:COG1080", "COG:G", "EC:2.7.3.9", "GO:0005737", "GO:0008965", "GO:0009401", "GO:0016301", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47668", "UniRef:UniRef90_P75168" ], "nt": "ATGAAAAAACTATCTGGTATCGGTGTATCTGATGGCATGGCCCTAGCCAAAGCCTTCCTTGTTAAAACACCGGAATTCGCTGTTAACAAATACCTCAAGCACCAATTAACGAAGGCGCAGGCCAAAAGACTCTTGGACTCAGCTTTCAAAAAAGCCGTTAAGGACTTAGAGGAAATTAAGGAAATTACCGTTAACAACATTAACACTGAAGCGGGCATGATCTTTGATGCGCACATTCAAATACTCAACGATCCCACGATCACCGAGCAACTAGAACAACAGTTAGCGCAAAATGTTCATCCTGTTATAGCCGTGGACACTGTCTTTAGCCAAACGGCTACCATGTTTAGCCAAATGCAGGACAAGTATTTCCAGGAACGTGCTGCAGACATTTTGGACTTGCGCCAACGCTTATTAGCTTATTTAACCGGACAAAAACCACATGACCTGGTCAAGATTAAGAGTGATGTCATTATTGTGGCCCATGACTTAACGCCGAGTCAAACTGCTACCCTTAACAAAAAATACGTCAAGGGTTTTTTAACCGAAATAGGTGGTCGTACCAGTCACGCTGCCATTATGGCGCGTTCACTGGAAATTCCCGCAGTGGTGGGCATTAAGGGCATTACTACTAAGGTCAAAGACGGTCAAATAGTTGGGGTTGATGGGCGCAAAGGAATAGCTGGCTTAGACCTAAACTCCAAGGACACTACCGAGTGGAAGAAACAAAAAGCGTTGGAAGAAAAATACCAACAAGAACTCAAGCAGTACACCAACAAGGAAACCGTGACTTTAGATGGTCATGCGGTTGTGGTGGCTGCTAACATTGGTAATGTGAAAGACATGGAATTGGCATGTCAGTACAATACCAACGGGGTTGGTTTATTCCGCACTGAGTTCCTCTACATGAACAGTCAAGAATGA", "aa": "MKKLSGIGVSDGMALAKAFLVKTPEFAVNKYLKHQLTKAQAKRLLDSAFKKAVKDLEEIKEITVNNINTEAGMIFDAHIQILNDPTITEQLEQQLAQNVHPVIAVDTVFSQTATMFSQMQDKYFQERAADILDLRQRLLAYLTGQKPHDLVKIKSDVIIVAHDLTPSQTATLNKKYVKGFLTEIGGRTSHAAIMARSLEIPAVVGIKGITTKVKDGQIVGVDGRKGIAGLDLNSKDTTEWKKQKALEEKYQQELKQYTNKETVTLDGHAVVVAANIGNVKDMELACQYNTNGVGLFRTEFLYMNSQE", "aa_hexdigest": "c1eb99295f1880dd5025e41e1a4dea03", "start_type": "ATG", "rbs_motif": "AATAA", "genes": [ "ptsI" ], "truncated": "5-prime", "pseudogene": { "start": 755074, "stop": 756300, "observations": { "mutation": [ 755379 ], "stop": [ 755379 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75168", "query_cov": 1.0, "subject_cov": 0.5367132867132867, "identity": 0.997, "score": 587.0, "evalue": 3.02e-207, "gene_start": 1, "gene_end": 307, "reference_start": 1, "reference_end": 307, "reference_sequence": "MKKLSGIGVSDGMALAKAFLVKTPEFAVNKYLKHQLTKAQAKRLLDSAFKKAVKDLEEIKEITVNNINTEAGMIFDAHIQMLNDPTITEQLEQQLAQNVHPVIAVDTVFSQTATMFSQMQDKYFQERAADILDLRQRLLAYLTGQKPHDLVKIKSDVIIVAHDLTPSQTATLNKKYVKGFLTEIGGRTSHAAIMARSLEIPAVVGIKGITTKVKDGQIVGVDGRKGIAGLDLNSKDTTEWKKQKALEEKYQQELKQYTNKETVTLDGHAVVVAANIGNVKDMELACQYNTNGVGLFRTEFLYMNSQEWPDEETQYQAYKAVLEQAHGDLVIIRTLDIGGDKKLNYYEFPHEDNPFLGYRALRLTLDKQDIFKTQLRALLRAADHGQLGIMFPMVATLDELLQAKQLLNQVHQELGGNKQFKLGIMIEIPAAVLAANTLSHHVDFFSIGTNDLIQYSFAADRMNKNVSYLYQPLNPALLKLIYLTIEGGKVNDIWTGMCGEMAGEPLAIPLLLGLGLKEFSMSASSMFKARMIIAKLNYTECQTLAQKALTLANAKEVEKLVEKFFKKKDIFI" }, "paralog": false, "identity": 0.9950980392156863, "score": 794.0, "evalue": 3.41e-291, "description": "Internal stop codon at 755379. Nonsense mutation around 755379.", "psc": { "uniref90_id": "UniRef90_P75168", "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "ec_ids": [ "2.7.3.9" ], "uniref50_id": "UniRef50_P47668", "cog_id": "COG1080", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75168", "EC:2.7.3.9", "UniRef:UniRef50_P47668", "COG:COG1080", "COG:G", "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47668", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47668" ], "product": "Phosphoenolpyruvate-protein phosphotransferase" } }, "id": "GIHJHMOALN_1338", "locus": "GCA009939745_01338" }, { "type": "cds", "sequence": "contig_1", "start": 756305, "stop": 757831, "strand": "-", "frame": 3, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0016020", "GO:0030145", "GO:0046537", "GO:0051919", "KEGG:K15633", "RefSeq:WP_014325644.1", "SO:0001217", "UniParc:UPI0001DD645C", "UniRef:UniRef100_A0AAX0SPX1", "UniRef:UniRef50_P75167", "UniRef:UniRef90_P75167" ], "nt": "ATGCATAAGAAGGTCTTACTAGCCATTTTGGATGGTTATGGTATTAGTAATAAGCAACACGGTAATGCGGTGTACCATGCTAAAACTCCAGCTTTAGACAGTTTGATTAAGGATTATCCCTGTGTCATGTTAGAAGCTTCGGGGGAAGCCGTTGGTCTACCACAAGGACAAATCGGTAACTCGGAAGTGGGTCACCTCAATATTGGGGCTGGTCGAATTGTTTACACTGGTTTATCGTTAATTAACCAAAACATTAAAACCGGGGCCTTTCACCACAACCAGGTGTTACTAGAGGCGATTGCTAGGGCCAAGGCTAACAATGCTAAGTTGCACTTAATTGGTCTGTTTTCCCATGGCGGGGTACACAGTCACATGGACCATTTGTATGCCTTGATTAAACTAGCAGCACCGCAGGTCAAAATGGTGCTCCACCTCTTTGGTGATGGGCGGGATGTAGCACCTTGCACGATGAAGAGTGACCTTGAAGCCTTCATGGTGTTCTTAAAGGATTACCACAATGTCATCATTGGTACCTTAGGTGGGCGTTACTATGGTATGGATCGTGACCAGCGCTGGGACAGAGAAGAGATTGCTTATAATGCCATTTTGGGTAACAGTAAGGCTAGCTTTACTGATCCGGTAGCTTATGTTCAAAGTGCCTATGACCAAAAGGTAACCGATGAGTTTTTGTACCCGGCTGTCAATGGTAATGTAGATAAAGAGCAGTTTGCGCTCAAAGATCATGACAGTGTGATCTTCTTTAACTTCCGTCCTGATCGTGCGCGGCAAATGTCACACATGCTCTTTCAAACAGATTACTATGACTACACCCCTAAAGCAGGACGAAAGCACAACCTCTTCTTTGTCACGATGATGAACTATGAGGGGATTAAACCAAGTGCCGTGGTCTTTCCGCCCGAAACTATTCCCAATACTTTTGGGGAAGTCATTGCGCACAATAAGTTAAAGCAACTGCGAATTGCAGAAACGGAAAAGTACGCCCATGTAACCTTCTTCTTTGATGGTGGAGTGGAAGTGGATCTACCCAATGAAACTAAGTGCATGGTACCTTCGTTAAAGGTGGCTACGTATGACTTAGCACCGGAAATGGCATGTAAGGGGATAACTGATCAATTACTAAATCAAATTAACCAGTTCGATTTGACGGTGTTGAACTTTGCCAACCCTGATATGGTAGGTCATACTGGTAACTATGCCGCTTGTGTGCAGGGCTTAGAGGCCTTGGATGTGCAAATACAGCGCATTATTGATTTCTGCAAGGCTAACCACATTACCCTGTTCTTAACTGCTGACCATGGTAACGCGGAAGAGATGATTGACAGCAACAATAACCCAGTTACCAAACACACTGTCAACAAGGTGCCGTTTGTATGTACTGATACAAACATTGACTTACAACAAGACAGTGCTAGTCTAGCCAATATTGCCCCAACCATTTTGGCTTACTTAGGGCTCAAACAGCCCGCAGAAATGACCGCAAACTCCCTTTTAATAAGTAAAAAGTAA", "aa": "MHKKVLLAILDGYGISNKQHGNAVYHAKTPALDSLIKDYPCVMLEASGEAVGLPQGQIGNSEVGHLNIGAGRIVYTGLSLINQNIKTGAFHHNQVLLEAIARAKANNAKLHLIGLFSHGGVHSHMDHLYALIKLAAPQVKMVLHLFGDGRDVAPCTMKSDLEAFMVFLKDYHNVIIGTLGGRYYGMDRDQRWDREEIAYNAILGNSKASFTDPVAYVQSAYDQKVTDEFLYPAVNGNVDKEQFALKDHDSVIFFNFRPDRARQMSHMLFQTDYYDYTPKAGRKHNLFFVTMMNYEGIKPSAVVFPPETIPNTFGEVIAHNKLKQLRIAETEKYAHVTFFFDGGVEVDLPNETKCMVPSLKVATYDLAPEMACKGITDQLLNQINQFDLTVLNFANPDMVGHTGNYAACVQGLEALDVQIQRIIDFCKANHITLFLTADHGNAEEMIDSNNNPVTKHTVNKVPFVCTDTNIDLQQDSASLANIAPTILAYLGLKQPAEMTANSLLISKK", "aa_hexdigest": "0c31da24fa2b730de551030ec80f6730", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD645C", "ncbi_nrp_id": "WP_014325644.1", "uniref100_id": "UniRef100_A0AAX0SPX1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD645C", "RefSeq:WP_014325644.1", "UniRef:UniRef100_A0AAX0SPX1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SPX1", "uniref90_id": "UniRef90_P75167" }, "psc": { "uniref90_id": "UniRef90_P75167", "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_P75167", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0006007", "GO:0006096", "GO:0016020", "GO:0030145", "GO:0046537", "GO:0051919" ] }, "pscc": { "uniref50_id": "UniRef50_P75167", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75167" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "GIHJHMOALN_1339", "locus": "GCA009939745_01339" }, { "type": "cds", "sequence": "contig_1", "start": 757824, "stop": 758006, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00121.24" ], "nt": "GTGTACCTTAGTGAACTGTACAACAAGGAAATTGCCCAACAAACCTCGATTCTCTATGGTGGTTCGGTTGCTAAGGACAACATTAAGGAGTTAGCACAAACCGAGCAAATTGATGGCTTTTTAGTGGGCAAGGCATCTTTGGATGTCAACGACTTTTTAGTAATGGCACAAGTATATGCATAA", "aa": "MYLSELYNKEIAQQTSILYGGSVAKDNIKELAQTEQIDGFLVGKASLDVNDFLVMAQVYA", "aa_hexdigest": "d51d19e6c8eaf0e8e0101c3f8fe5ec8f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00121.24", "name": "TIM", "length": 55, "aa_cov": 0.9166666666666666, "hmm_cov": 0.9818181818181818, "evalue": 7.00881635367173e-18, "score": 60.47081756591797, "start": 3, "stop": 57 } ], "seq_stats": { "molecular_weight": 6645.474500000002, "isoelectric_point": 4.285114479064942 }, "id": "GIHJHMOALN_1340", "locus": "GCA009939745_01340" }, { "type": "cds", "sequence": "contig_1", "start": 758070, "stop": 758492, "strand": "-", "frame": 2, "gene": "tpiA", "product": "triose-phosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_P78010", "UniRef:UniRef90_P78010" ], "nt": "GTGGAGCAATTTAAACAGCACCCTGCTAAAACTAAGGCGGTTTTGGGTATTGCCCCAGTCCACGTCCACTTAACCGAGGTAAACAAAGTCTTGCCTAATAACCTCTTACTGTTAGCCCAAGACGCTAACTTTATTGCTAGTGGTTCTTACACTGGTACGGTGAGCTATACCCAACTGCAAGACATTAAGGTCAACAGTGTCATTATTGGTCACTCGGAACGGCGAAAGTACTTTAACGAAACAGCGCAAGTTATTAATCAAAAACTAAAAGCCTGTTTACAAGCTGGAATGTTAGTCGTGCTCTGTATTGGTGAAACGGAAGGTCAACCAATTAGTTTTCTCAAAGAAGACTTAACCCAAGTCTTGCAAGGCATTGACCTAAGCTTACTCAAACAGTTAGTGATTGCTTACGAACCAATTTGA", "aa": "MEQFKQHPAKTKAVLGIAPVHVHLTEVNKVLPNNLLLLAQDANFIASGSYTGTVSYTQLQDIKVNSVIIGHSERRKYFNETAQVINQKLKACLQAGMLVVLCIGETEGQPISFLKEDLTQVLQGIDLSLLKQLVIAYEPI", "aa_hexdigest": "9c5023fcb743242a885b5d55a7d0cbe1", "start_type": "GTG", "rbs_motif": null, "genes": [ "tpiA" ], "truncated": "both", "pseudogene": { "start": 757824, "stop": 758558, "observations": { "mutation": [ 758072, 758531 ], "stop": [ 758072, 758531 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78010", "query_cov": 1.0, "subject_cov": 0.5737704918032787, "identity": 0.993, "score": 271.0, "evalue": 1.55e-90, "gene_start": 1, "gene_end": 140, "reference_start": 23, "reference_end": 162, "reference_sequence": "MRTKYLIGNWKTNKDLHQALAFVEQFKQHPAKTKAVLGIAPVHVHLTEVNKVLPNNLLLLAQDANFIASGSYTGTVSYTQLQDIKVNSVIIGHSERRKYFNETAQVINQKLKACLQAGMLVVLCIGETEGQPISFLKEDLTQVLQGIDLSLLKQLVIAYEPIWAIGTGKTATPEIANNTIAQIRVYLSELYNKEIAQQTSILYGGSVAKDNIKELAQTEQIDGFLVGKASLDVNDFLVMAQVYA" }, "paralog": false, "identity": 0.9918032786885246, "score": 466.0, "evalue": 1.43e-169, "description": "Internal stop codon at 758072, 758531. Nonsense mutation around 758072, 758531.", "psc": { "uniref90_id": "UniRef90_P78010", "gene": "tpiA", "product": "triose-phosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_P78010", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78010", "EC:5.3.1.1", "UniRef:UniRef50_P78010", "COG:COG0149", "COG:G", "KEGG:K01803", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ] }, "pscc": { "uniref50_id": "UniRef50_P78010", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78010" ], "product": "Triosephosphate isomerase" } }, "id": "GIHJHMOALN_1341", "locus": "GCA009939745_01341" }, { "type": "cds", "sequence": "contig_1", "start": 758611, "stop": 759825, "strand": "-", "frame": 1, "gene": "yitT", "product": "putative MG432-like protein", "db_xrefs": [ "COG:COG1284", "COG:S", "GO:0005886", "RefSeq:WP_014325645.1", "SO:0001217", "UniParc:UPI0002386E42", "UniRef:UniRef100_A0AB38W7K2", "UniRef:UniRef50_Q49432", "UniRef:UniRef90_P75166" ], "nt": "ATGCAAGATAAAAACGTCAAAATTCAGGGCAATCTGGTACGGGTACACCTTTCGGGATCGTTTCTGAAGTTCCAGGCAATTTACAAGGTGAAAAAGCTGTACTTACAGCTGTTAATTCTCTCCGTGATTGCCTTCTTTTGGGGCTTGTTAGGGGTTGTGTTTGTCCAGTTTTCTGGATTATATGACATTGGCATTGCTTCCATTAGTCAGGGCTTAGCACGGTTAGCGGATTATTTAATTAGGTCGAACAAGGTCAGTGTGGATGCTGACACCATTTACAACGTCATCTTCTGGTTGAGTCAAATTCTGATTAACATTCCCTTATTTGTTTTGGGTTGGTACAAGATTTCCAAAAAGTTTACCTTGTTAACCCTTTACTTTGTGGTAGTCTCCAACGTTTTTGGGTTTGCCTTCTCTTACATTCCGGGCGTGGAAAACTTCTTCTTGTTTGCTAATTTAACTGAACTTACTAAGGCCAACGGTGGCTTAGAACAAGCGATTAACAACCAAGGGGTGCAACTGATCTTTTGGGAACAAACCGCTGAAAAGCAAATTTCGTTAATGTTCTATGCGCTGATCTGGGGTTTTCTTCAAGCTGTGTTTTACTCAGTTATCCTAATTATTGATGCATCGAGTGGTGGGTTGGACTTTTTGGCCTTCTGGTATTCGGAAAAGAAACACAAGGACATTGGTGGTATTTTGTTTATTGTTAACACCCTTAGTTTCTTGATCGGTTACACCATTGGCACTTACCTTACCGGTAGCTTACTAGCACAAGGCTTTCAAGGAGATAGACAAAAACCGTTTGGAGTGGCTTTTTTCTTGTCCCCTAACTTAGTGTTTACGATCTTCATGAACATTATCTTAGGGATCTTTACCTCCTACTTCTTTCCTAAATACCAGTTTGTCAAAGTGGAAGTGTATGGTAAACACATGGAACAAATGCGCAACTACTTGTTGAGCAGTAACCAGTCCTTTGCGGTCACTATGTTCGAAGTGGAAGGGGGGTATTCGCGCCAAAAGAACCAGGTGTTAGTTACAAACTGTTTGTTTACGAAAACGGCCGAACTTTTAGAAGCTGTTAGACGAGTCGATCCGGATGCTCTGTTCTCAATTACCTTCATTAAAAAGTTGGATGGTTATATCTATGAAAGAAAAGCACCTGATAAAGTAGTCCCACCAGTAAAAGACCCAGTTAAAGCCCAGGAAAATTAA", "aa": "MQDKNVKIQGNLVRVHLSGSFLKFQAIYKVKKLYLQLLILSVIAFFWGLLGVVFVQFSGLYDIGIASISQGLARLADYLIRSNKVSVDADTIYNVIFWLSQILINIPLFVLGWYKISKKFTLLTLYFVVVSNVFGFAFSYIPGVENFFLFANLTELTKANGGLEQAINNQGVQLIFWEQTAEKQISLMFYALIWGFLQAVFYSVILIIDASSGGLDFLAFWYSEKKHKDIGGILFIVNTLSFLIGYTIGTYLTGSLLAQGFQGDRQKPFGVAFFLSPNLVFTIFMNIILGIFTSYFFPKYQFVKVEVYGKHMEQMRNYLLSSNQSFAVTMFEVEGGYSRQKNQVLVTNCLFTKTAELLEAVRRVDPDALFSITFIKKLDGYIYERKAPDKVVPPVKDPVKAQEN", "aa_hexdigest": "83eb33bcaa37ec7b0a27224995c053a9", "start_type": "ATG", "rbs_motif": "AAAAA", "ups": { "uniparc_id": "UPI0002386E42", "ncbi_nrp_id": "WP_014325645.1", "uniref100_id": "UniRef100_A0AB38W7K2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386E42", "RefSeq:WP_014325645.1", "UniRef:UniRef100_A0AB38W7K2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7K2", "uniref90_id": "UniRef90_P75166" }, "psc": { "uniref90_id": "UniRef90_P75166", "gene": "YitT", "product": "Uncharacterized protein MG432 homolog", "uniref50_id": "UniRef50_Q49432", "cog_id": "COG1284", "cog_category": "S", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q49432", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49432" ], "product": "Uncharacterized protein MG432" }, "genes": [ "yitT" ], "id": "GIHJHMOALN_1342", "locus": "GCA009939745_01342" }, { "type": "cds", "sequence": "contig_1", "start": 759912, "stop": 760028, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACAAAAATAGAGCTCATAAAAGAACTACGTAAAACAACACAAGCGAGCATGATGGACTGTAAAAAGGCTTTGGAACAAAATAACGATGATCTCGAAAAGGCCATTAAGTGA", "aa": "MATKIELIKELRKTTQASMMDCKKALEQNNDDLEKAIK", "aa_hexdigest": "f0cb3a4a31ad7258ac8d1163fc305f1b", "start_type": "ATG", "rbs_motif": "AAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4381.1236, "isoelectric_point": 7.870574378967285 }, "id": "GIHJHMOALN_1343", "locus": "GCA009939745_01343" }, { "type": "cds", "sequence": "contig_1", "start": 760029, "stop": 760808, "strand": "+", "frame": 3, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "SO:0001217", "UniRef:UniRef50_P47246", "UniRef:UniRef90_P78009" ], "nt": "TTGAGAGAAAACGGAATTGTTAAATCTGCTAAGAAATTAGGCAAAGTAGCCGCTGATGGTTGCATTGTGTTACACAGTGATCACCACAAGGCAGTGATGGTGGAAATTAATTCTCAAACGGACTTTGTAGCGCGTAGTCAAGAGTTAACGGACTTTGCGCAATTAATGATTAGTGAAGTCTTTAAAAAGGCTACCCCTACCACTACTATTGAAGAAGTAACCCAGTACGAACTCCAAGGCAAAGAAAAGGTAGCAGAACGTTTGGCTTTAGTCGCTTCCAAAACTGATGAAAAGATTGTGTTGCGCCGCTTAATGGTGTTTGAAAGCAAAACTAACCACATCTTTAGCTACCTCCACGCTAACAAACGGATTGGCGTAATCTTGGAAGTGGAAGGCAAGTTTGATGAACAAGATGGTAAACACTTAGCAATGCACATTGCCGCTAACTCACCGCAGTTCATTGATCAGGACAATGTGGATCAAACATGGTTAGCTAACGAAACCAGCATCATTAAGTCCCAAGCCAAACTAGAAGTCCAAGACAATCCGAAGAAAGCAGCGTTCTTAGACAAAACTATTGCTGGACGCGTGAACAAACTCTTAATTGACATTTGTCTGGTAAACCAAAAGTACTTGGTCGATGAATCCAAAACAGTTGGTCAATTCTTAAAGGAAAAGAACAGTAAAGTAATCCATTTTGTGCGCTTTGAAGTTGGTGAAGGCATTGTCAAAGAAGCAGTTGACTTCGCTAGTGAAGTAAGTGCGCAAATGAAAAAATAA", "aa": "MRENGIVKSAKKLGKVAADGCIVLHSDHHKAVMVEINSQTDFVARSQELTDFAQLMISEVFKKATPTTTIEEVTQYELQGKEKVAERLALVASKTDEKIVLRRLMVFESKTNHIFSYLHANKRIGVILEVEGKFDEQDGKHLAMHIAANSPQFIDQDNVDQTWLANETSIIKSQAKLEVQDNPKKAAFLDKTIAGRVNKLLIDICLVNQKYLVDESKTVGQFLKEKNSKVIHFVRFEVGEGIVKEAVDFASEVSAQMKK", "aa_hexdigest": "bdf2184fb757c8bf8769a32d2c5e8e8d", "start_type": "TTG", "rbs_motif": "AAAA", "psc": { "uniref90_id": "UniRef90_P78009", "query_cov": 1.0, "subject_cov": 0.8691275167785235, "identity": 0.996, "score": 496.0, "evalue": 2.1e-176, "valid": true, "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_P47246", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_P47246", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47246" ], "product": "Elongation factor Ts" }, "genes": [ "tsf" ], "id": "GIHJHMOALN_1344", "locus": "GCA009939745_01344" }, { "type": "cds", "sequence": "contig_1", "start": 760810, "stop": 761517, "strand": "+", "frame": 1, "gene": "pyrH", "product": "UMP kinase", "db_xrefs": [ "COG:COG0528", "COG:F", "EC:2.7.4.22", "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210", "KEGG:K09903", "SO:0001217", "UniParc:UPI0001DD6475", "UniRef:UniRef100_A0AAN4XGQ3", "UniRef:UniRef50_P47672", "UniRef:UniRef90_P75165" ], "nt": "ATGCGCCTAAAGATCCTAATCAAACTAAGCGGTGCTGGCATGAGCGCTGATAGTTCCGAACCGTTTTCCAACCAATTTTTAGAAACTGTCATCGCTCAACTAAAACAACTAGTACCCAACTACCAAATAGGCATTGTGATTGGTGGGGGCAACATTATGCGCGGTAAGTCTTGTAGTGACTACAACATTACCGAAATAGCAGGGCACCACTTGGGCATTATGGCCACGGTTATTAACGGCGCTTTTTTAAAGGCTAAGTTTGATAGTCATAAACTTAACAGCACCCTGTTGAGTGCAGTGAGCTGTCCTAGTTTAGCGACCCACATTGTGTCCCAGGCCACGATTGATGATGCCTTTAAAAACCATGACATTGTCATTTTTGCGGGCGGTACTGGTAACCCGTACTTTTCGACTGATACCGCTGTGGCGTTGCGTGCTACCCAAATGCAAGCCGATATTATTCTAATTGGCAAGAATGGTGTTGATGGGGTTTACACTGCGGATCCTAAAAAGGATAAGCATGCTAAGTTTTTAGCTAGCTTAACTTACGCTGAAGCGATTAAGAATGACCTGCAAATCATGGACATTACCGCCTTTACAATGTGTAAGGAAAATAACTTAAAGGTGATCATATTTAACATCAATGCCGAGCATGCCATCATTAAGGCATTGAGCAAACAAGGTAAATACACCTTAATAGAAAAATAA", "aa": "MRLKILIKLSGAGMSADSSEPFSNQFLETVIAQLKQLVPNYQIGIVIGGGNIMRGKSCSDYNITEIAGHHLGIMATVINGAFLKAKFDSHKLNSTLLSAVSCPSLATHIVSQATIDDAFKNHDIVIFAGGTGNPYFSTDTAVALRATQMQADIILIGKNGVDGVYTADPKKDKHAKFLASLTYAEAIKNDLQIMDITAFTMCKENNLKVIIFNINAEHAIIKALSKQGKYTLIEK", "aa_hexdigest": "510b04b8c0c148efd9efe0ac5d887d14", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6475", "uniref100_id": "UniRef100_A0AAN4XGQ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6475", "UniRef:UniRef100_A0AAN4XGQ3" ] }, "ips": { "uniref100_id": "UniRef100_A0AAN4XGQ3", "uniref90_id": "UniRef90_P75165" }, "psc": { "uniref90_id": "UniRef90_P75165", "gene": "pyrH", "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_P47672", "cog_id": "COG0528", "cog_category": "F", "kegg_orthology_id": "K09903", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210" ] }, "pscc": { "uniref50_id": "UniRef50_P47672", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47672" ], "product": "Uridylate kinase" }, "genes": [ "pyrH" ], "id": "GIHJHMOALN_1345", "locus": "GCA009939745_01345" }, { "type": "cds", "sequence": "contig_1", "start": 761689, "stop": 762474, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4BAEE8", "UniRef:UniRef90_UPI000A4BAEE8" ], "nt": "TTGATTGCCAATGCGATTGATGAACACCGCTTAAGCAACACCGCTTTCCCCGTAATTGAATTGCAAAAAGGCTTTCTAAACTCTTCTTTAGTGATTAAAGATTATGGTAGGGGTATTAAGTCCAATCACTTTATCCAAAACGAAAGTAGGGAAAAGGTTCAAAGTGAAAAAACTATTGGTAAGTTTGGCATTGGTCTCAAAGATGCCATAGCTGTTCTTTTCCGTCATAATGTCAAAGTTAGCTTTACCAGTAGTGAAGGCACTTTTACCCCTGTTGAGCGAATGAAGGAAGGTATGAAAGACGGCACCAAAACAATTCAAATAACAGTGGATGAAACCAAGAAGATTGACAAGGGCACTGACATCCTCATTAGCAAGATTAGTAGAAGTGATTACGAAAAAGCCATTGCTATTTTTTTGGAATTAAGAACTGGCTATCAAAAACTGGCTTCTTCCAAAAAGGGTGATATTTATCGCAGTGAAAATGGTTCAGAGATCTTTCTTAACGGGATGAAAATCGGAACCGATGAAAACTTCTTGTTTTCTTATGACATTAAAGAACCCAATAAGAAATTGCAAAAATCCTTAAACCGGGAGCGTAAAACACTTTCCCGTGATAGCTACCGTGACAATATCATTTCCATTTTGAAGTCCAGCATTAATAAAAATACCCAAACTTTAATTAACCAATTAATTGACAACCGCGATCAGTTTGAAACTGGTGAATGGAGTTTTATTGATGTCAAAAAGTTAGTAATGCAAAACACTAACCGAAAGATTTTGTGA", "aa": "MIANAIDEHRLSNTAFPVIELQKGFLNSSLVIKDYGRGIKSNHFIQNESREKVQSEKTIGKFGIGLKDAIAVLFRHNVKVSFTSSEGTFTPVERMKEGMKDGTKTIQITVDETKKIDKGTDILISKISRSDYEKAIAIFLELRTGYQKLASSKKGDIYRSENGSEIFLNGMKIGTDENFLFSYDIKEPNKKLQKSLNRERKTLSRDSYRDNIISILKSSINKNTQTLINQLIDNRDQFETGEWSFIDVKKLVMQNTNRKIL", "aa_hexdigest": "9c79440ad272f34732688b266ca1f096", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A4BAEE8", "query_cov": 0.9272030651340997, "subject_cov": 0.8642857142857143, "identity": 0.992, "score": 458.0, "evalue": 1.01e-161, "valid": true, "product": "ATP-binding protein", "uniref50_id": "UniRef50_UPI000A4BAEE8" }, "pscc": { "uniref50_id": "UniRef50_UPI000A4BAEE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A4BAEE8" ], "product": "ATP-binding protein" }, "genes": [], "id": "GIHJHMOALN_1346", "locus": "GCA009939745_01346" }, { "type": "cds", "sequence": "contig_1", "start": 762577, "stop": 762741, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATGATGCCGAATTTAAAAGCTATACCCTCAACGATTTTGGTGATAGATTTGTCAATGAATTCCAAACGGAAGAGGTGCCATTCCACAAATTAACTGAGATCGAAAAAGACAACTGGAATTGGGTAATGGCTAAGGTCAAAGAACTTACCAGGGTTTGA", "aa": "MENDAEFKSYTLNDFGDRFVNEFQTEEVPFHKLTEIEKDNWNWVMAKVKELTRV", "aa_hexdigest": "65a8f1754f43d7a31f7033ce0dddd929", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6570.2459, "isoelectric_point": 4.637345695495605 }, "id": "GIHJHMOALN_1347", "locus": "GCA009939745_01347" }, { "type": "cds", "sequence": "contig_1", "start": 763157, "stop": 763258, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00035C1894", "UniRef:UniRef100_A0AB38W6X6", "UniRef:UniRef50_A0AB38W6X6", "UniRef:UniRef90_A0AB38W6X6" ], "nt": "ATGTTTTATCTCGATTTAAACATTCAAAATATTCTTACCAATTGGGAGATAGCTGATGCTGTTCGCGAACTGATCGCTAACGCCATTGATGAGCACATTTAA", "aa": "MFYLDLNIQNILTNWEIADAVRELIANAIDEHI", "aa_hexdigest": "be05d12b720a62bb5b8bcb4ad1f3b971", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00035C1894", "uniref100_id": "UniRef100_A0AB38W6X6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00035C1894", "UniRef:UniRef100_A0AB38W6X6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6X6", "uniref90_id": "UniRef90_A0AB38W6X6" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6X6", "uniref50_id": "UniRef50_A0AB38W6X6" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6X6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6X6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3872.3594999999987, "isoelectric_point": 4.0500284194946286 }, "id": "GIHJHMOALN_1348", "locus": "GCA009939745_01348" }, { "type": "cds", "sequence": "contig_1", "start": 763462, "stop": 764019, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SSK4", "UniRef:UniRef90_A0AAX0SSK4" ], "nt": "GTGAGTTTTACAAGTCAGCAAGGTACTTTTATTCCTGTAGAACGGAGTAAAACTGAACTGGAAGGTAGCATCAAAACAATTCAAATGGAAGTTGATCCAGCTGAACAAATTGAAAAAGCGGGTACCTTAATCACAATTGAAGGATTGAAAAAAGATGACTATGACAAAGCTATTGTTAATTTCTTAGCTCTACGGGAAGATTTGCAATTATTAGCTGCTTCACCCAAAGGCGATGTTTACCGTAATACATCAGGTAACGGCGCAGAAATTTTTCTCAACGGGATGAAAATTGCAACCGATGAAGACTTTCTCTTTTCCTATCACATTAAAGAACCTAACAAAAAGTTACAGCGTTCCCTTAACCGGGAAAATAAAAATTTACCAAGAGATTGTTATCGTGAAAACATCATCACCATTCTTAAGTCCAACATTAACAATAGAACACAAACACTTATTGATGAATTAATAGATTCACGTGACCAATATGACAATGGCGAGTGGAGTTTTATTGATGTCAAAAAACTAATTGGTTTAAACACAAACAGAAATATTTTGTGA", "aa": "MSFTSQQGTFIPVERSKTELEGSIKTIQMEVDPAEQIEKAGTLITIEGLKKDDYDKAIVNFLALREDLQLLAASPKGDVYRNTSGNGAEIFLNGMKIATDEDFLFSYHIKEPNKKLQRSLNRENKNLPRDCYRENIITILKSNINNRTQTLIDELIDSRDQYDNGEWSFIDVKKLIGLNTNRNIL", "aa_hexdigest": "7fbf0fa706b5bb500aed686f813f1f3d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAX0SSK4", "query_cov": 1.0, "subject_cov": 0.9296482412060302, "identity": 1.0, "score": 358.0, "evalue": 7.67e-125, "valid": true, "uniref50_id": "UniRef50_A0AAX0SSK4" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SSK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SSK4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21158.681400000016, "isoelectric_point": 5.207952308654785 }, "id": "GIHJHMOALN_1349", "locus": "GCA009939745_01349" }, { "type": "cds", "sequence": "contig_1", "start": 764071, "stop": 764310, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACCAAAAAATATGAAATTTTGGCGTTAAATTCCTTGCAATACAGAAGCATGGAAAACGATGATCGTTTAAAAAAGCAAACACTGATGCATGTAAGTGAAAAGTTAAAACAACAAAGAATTGAAGAAGAGACTGAGAAAATCAAAGTTAAAGAACAAAAGCCACGAAAACGATTTGAGGAAGAAGATTTGCCAATAGAAGATTTAAATCCAATTGAAAGAGAAGGTTGGGATTGA", "aa": "MDTKKYEILALNSLQYRSMENDDRLKKQTLMHVSEKLKQQRIEEETEKIKVKEQKPRKRFEEEDLPIEDLNPIEREGWD", "aa_hexdigest": "6c9fa4400e08a790e0edac72f2fce74f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9689.885599999996, "isoelectric_point": 5.302191352844238 }, "id": "GIHJHMOALN_1350", "locus": "GCA009939745_01350" }, { "type": "cds", "sequence": "contig_1", "start": 764314, "stop": 764604, "strand": "+", "frame": 1, "gene": null, "product": "SprT-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S5E5", "UniRef:UniRef90_A0AAX0S5E5" ], "nt": "ATGGAAAAGGCCAGAGAATTATGTGGTTTTATAAGAGGATGGGAAAAACTTTATGAAGAATATCAGTTTGTTCTAATGGAAAAGAATCACAAATATGTCGGGCTTTGTTACACCGATCAAAAAATTATCAAACTCTCAAGAGAAATATTGAAAGATGAATACAGTTTATTGAATACCTTAGTGCATGAAATTTGTCATGCAACAACTAATGGTCGAGACGGAACGAAGAAGTTTGAAAGGGGTCTAACTGATGCTTTTCATCCATTATTTAAACTAGGGCAAAGTAAATAA", "aa": "MEKARELCGFIRGWEKLYEEYQFVLMEKNHKYVGLCYTDQKIIKLSREILKDEYSLLNTLVHEICHATTNGRDGTKKFERGLTDAFHPLFKLGQSK", "aa_hexdigest": "1911e99f4110e194e22ba1ec1d261d48", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 764020, "stop": 764604, "observations": { "mutation": [ 764059, 764308 ], "stop": [ 764059, 764308 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AAX0S5E5", "query_cov": 1.0, "subject_cov": 0.4485981308411215, "identity": 0.99, "score": 202.0, "evalue": 1.6e-64, "gene_start": 1, "gene_end": 96, "reference_start": 119, "reference_end": 214, "reference_sequence": "MEFYDVKKLIGLNTNRNILWADSSSKNIEKLIYSLYGMDTKKYEILALNSLQYRSMENDDRLKKQTLMHVSEKLKQQRIEEEAEKIKVKEQKPRKRFEEEDLPIEDLNPIEREGWDWAMEKARELCGFIRGWEKLYEEYQFVLMEKNHKYVGLCYTDQKIIKLSRGILKDEYSLLNTLVHEICHATTNGRDGTKKFERGLTDAFHPLFKLGQSK" }, "paralog": false, "identity": 0.979381443298969, "score": 383.0, "evalue": 3.39e-138, "description": "Internal stop codon at 764059, 764308. Nonsense mutation around 764059, 764308.", "psc": { "uniref90_id": "UniRef90_A0AAX0S5E5", "product": "SprT-like domain-containing protein", "uniref50_id": "UniRef50_A0AAX0S5E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AAX0S5E5", "UniRef:UniRef50_A0AAX0S5E5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAX0S5E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0S5E5" ], "product": "SprT-like domain-containing protein" } }, "id": "GIHJHMOALN_1351", "locus": "GCA009939745_01351" }, { "type": "cds", "sequence": "contig_1", "start": 764753, "stop": 765235, "strand": "+", "frame": 2, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023", "SO:0001217", "UniRef:UniRef50_P47673", "UniRef:UniRef90_P75161" ], "nt": "TTGAGCAAAATCCGTACTGGTAGACCTAATCCAAAACTGTTTGATAATATCCTTGTGGAAAGCTATGGTGACAGAATGCCAATGGTAGCATTAGCACAAATTGCGGTAAATCCACCGCGGGAAATTGTGATCAAGCCGTTTGACGTTAAAAACAATATTAATGCCATCTACAGCGAAATTCAACGTGCTAACTTAGGTGTACAACCAGTGATTGACGGAGATAAAATTCGCATTAACTTTCCCCCAATGACCCAAGAAAGTCGTTTGGAAAGCATTAAGCAAGCTAAAAAAGTAGTGGAACAAATCCACCAAGAGTTGCGCAGTGTGCGACGTGATACCCTTCAAATGATTAAGAAGGATGATCATAAAGACGAAGACTTTGAAGAATTCTTAAAAGAAGAAGTAGAAAAAGTTAACAAGCAGTATATTGCACAGTTAGAAACTATTCAAAAACAAAAAGAAAAAGAACTATTAGTGGTCTAA", "aa": "MSKIRTGRPNPKLFDNILVESYGDRMPMVALAQIAVNPPREIVIKPFDVKNNINAIYSEIQRANLGVQPVIDGDKIRINFPPMTQESRLESIKQAKKVVEQIHQELRSVRRDTLQMIKKDDHKDEDFEEFLKEEVEKVNKQYIAQLETIQKQKEKELLVV", "aa_hexdigest": "707cd85388fc8f405b301a1785ff56b9", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75161", "query_cov": 1.0, "subject_cov": 0.8648648648648649, "identity": 0.9940000000000001, "score": 299.0, "evalue": 5.07e-102, "valid": true, "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_P47673", "cog_id": "COG0233", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ] }, "pscc": { "uniref50_id": "UniRef50_P47673", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47673" ], "product": "Ribosome-recycling factor" }, "genes": [ "frr" ], "id": "GIHJHMOALN_1352", "locus": "GCA009939745_01352" }, { "type": "cds", "sequence": "contig_1", "start": 765439, "stop": 765648, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATCCTGACTTTAAGGCAGTCCGTTTTGCTTTTGGCTTAGTGATAGTTTTATTTATTAGCGTTATTGCCGTTTTAATGAATTGGGAGTTGTCTAACAGTATCTTTAAAAACTATGAAAAGCTCAACTTATCCTTATCACTACTTAGTGGAATTATGGTTTCAGGTGGGATGATTCCAACCTTTTTCGTAATCTATTTCCGTGAATGA", "aa": "MHPDFKAVRFAFGLVIVLFISVIAVLMNWELSNSIFKNYEKLNLSLSLLSGIMVSGGMIPTFFVIYFRE", "aa_hexdigest": "7833b4eeb50329d2239de1359567d57b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7876.366299999994, "isoelectric_point": 8.16248722076416 }, "id": "GIHJHMOALN_1353", "locus": "GCA009939745_01353" }, { "type": "cds", "sequence": "contig_1", "start": 765691, "stop": 766167, "strand": "+", "frame": 1, "gene": "cdsA", "product": "Putative phosphatidate cytidylyltransferase", "db_xrefs": [ "COG:COG0575", "COG:I", "EC:2.7.7.41", "GO:0004605", "GO:0005886", "GO:0016024", "KEGG:K00981", "SO:0001217", "UniRef:UniRef50_Q49433", "UniRef:UniRef90_P75160" ], "nt": "ATGATTGTCTTTTTATGGGCAGTGTACATGATTAGCACTTCCTTCATAAAGATCCGACCATCATTGCAAGTGATTTATAGCCTAGGTGCAGTGATCTGTTTCATCGCTTGCATAGGTACAATTTACTTTTCCGTAATTAGGGGATGGACTACCATTTTTCTCTTAATTGCTATTGGCGTTTGCACTGATACCTTTGCTTATCTGTTTGGTAAACGTTTTGGAAAAAATCCCCTCATTAAAATTTCTCCCTCAAAAACTTGGGAAGGTGCTTTCTTTGGGGTTACTGGTACAGTTTTAACCATTTCTATTATTTGTGTTTTGTACTCGATCCCTAACTATGTAAGACAACCTAGCATAAAGGATGCAAGTAAGACAGCACTACAAACACCACAAAACTATGATGTGCATAACCTTATTACCAATGTCTTTCTCATCTCCTTCATTTCCGGTGGTAGTACCTTTTACATCTACTGGTGA", "aa": "MIVFLWAVYMISTSFIKIRPSLQVIYSLGAVICFIACIGTIYFSVIRGWTTIFLLIAIGVCTDTFAYLFGKRFGKNPLIKISPSKTWEGAFFGVTGTVLTISIICVLYSIPNYVRQPSIKDASKTALQTPQNYDVHNLITNVFLISFISGGSTFYIYW", "aa_hexdigest": "c40ab368dd201c84e57d8d7d9d61fa57", "start_type": "ATG", "rbs_motif": null, "genes": [ "cdsA" ], "truncated": "both", "pseudogene": { "start": 765391, "stop": 766422, "observations": { "mutation": [ 765646, 765664, 765670, 766165 ], "stop": [ 765646, 765664, 765670, 766165 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75160", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 312.0, "evalue": 2.44e-104, "gene_start": 1, "gene_end": 158, "reference_start": 153, "reference_end": 310, "reference_sequence": "MDLKDNSFAKKRSTVFVVLLIVFCFFLMFSAFADGFNFWSPWSTDFNSRVIHIKSDGNIETTSIVSHELHPDFKAVRFAFGLVIVLFISVIAVLMNWELSNSIFKNYEKLNLSLSLLSGIMVSGGMIPTFFVIYFREWNATVNWIWTASFAGMIVFLWAVYMISTSFIKIRPSLQVIYSLGAVICFIACIGTIYFSVIRGWTTIFLLIAIGVCTDTFAYLFGKRFGKNPLIKISPSKTWEGAFFGVTGTVLTISIICVLYSIPNYVRQPSIKDASKTALQTPQNYDVHNLITNVFLISFISGGSTFYIYWWVSTLALIFTASIFAIGGDLFFSYIKRLTKIKDFSKLLGKHGGILDRFDSSSFLISFFFIYHVIAGISSNQRLLMEPNTYFSAVS" }, "paralog": false, "identity": 0.9854227405247813, "score": 660.0, "evalue": 1.29e-243, "description": "Internal stop codon at 765646, 765664, 765670, 766165. Nonsense mutation around 765646, 765664, 765670, 766165.", "psc": { "uniref90_id": "UniRef90_P75160", "gene": "cdsA", "product": "Putative phosphatidate cytidylyltransferase", "ec_ids": [ "2.7.7.41" ], "uniref50_id": "UniRef50_Q49433", "cog_id": "COG0575", "cog_category": "I", "kegg_orthology_id": "K00981", "go_ids": [ "GO:0005886", "GO:0004605", "GO:0016024" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75160", "EC:2.7.7.41", "UniRef:UniRef50_Q49433", "COG:COG0575", "COG:I", "KEGG:K00981", "GO:0005886", "GO:0004605", "GO:0016024" ] }, "pscc": { "uniref50_id": "UniRef50_Q49433", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49433" ], "product": "Putative phosphatidate cytidylyltransferase" } }, "id": "GIHJHMOALN_1354", "locus": "GCA009939745_01354" }, { "type": "cds", "sequence": "contig_1", "start": 766526, "stop": 767017, "strand": "+", "frame": 2, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF638P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_P75159" ], "nt": "ATGCTCGAAAAAGAGCTATTGGCGCCCGATGGTAAATACGAATACTTTAACGGTGGTATTAAAGCCTCTGGACGCACCAATGAGTTCAATACCTTTAAGAACACCATTAGCATTATCATAGGCGGTTCTTGTGGTTATGTCAGATTAGCCGATAAGGATTACTTCTGTGGCCAGAGTAGCTGTACCCTAACTGTTTTAGATCCCCTGGAAATTGACCTCAAGTTTGCTTATTATGCACTTAAATCACAAGAGGAAAAAATTACCAGTTTAGCTAGCGGAACTACCATTAAAAACATTCGCCTAAGTGATTTAAAGGATTTACCTATTCCGCTTGTTAAAAGCATTCAGGATCAACGAACTATTGCACATGCTTTAAGTGTGTTTGACCTTCGCATCGAACACCTCAATGAATTAATTGAAGTAAACCGTAAGCTCAGAGACGAGTATGCCCACAAATTATTTACCTTAGATCCAGACTTCTTAACACACTGA", "aa": "MLEKELLAPDGKYEYFNGGIKASGRTNEFNTFKNTISIIIGGSCGYVRLADKDYFCGQSSCTLTVLDPLEIDLKFAYYALKSQEEKITSLASGTTIKNIRLSDLKDLPIPLVKSIQDQRTIAHALSVFDLRIEHLNELIEVNRKLRDEYAHKLFTLDPDFLTH", "aa_hexdigest": "a1858b4425475498eaa5bee35fab524d", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 766442, "stop": 767320, "observations": { "mutation": [ 766478, 767015 ], "stop": [ 766478, 767015 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75159", "query_cov": 1.0, "subject_cov": 0.43466666666666665, "identity": 1.0, "score": 328.0, "evalue": 6.72e-111, "gene_start": 1, "gene_end": 163, "reference_start": 29, "reference_end": 191, "reference_sequence": "MTPKLKLNTNSNWTKKTLGSLFELKKGEMLEKELLAPDGKYEYFNGGIKASGRTNEFNTFKNTISIIIGGSCGYVRLADKDYFCGQSSCTLTVLDPLEIDLKFAYYALKSQEEKITSLASGTTIKNIRLSDLKDLPIPLVKSIQDQRTIAHALSVFDLRIEHLNELIEVNRKLRDEYAHKLFTLDPDFLTHWNLHELHEQMGEISLGEVFHLKSGKYLKADERFEDGKFPYYGAGIESTSFVNEPNTKGDTLSMIANGYSIGNIRYHTIPWFNGTGGIAMEALKPNKTYVPFFYCALKYMQKDLKERFKRDESPFISLKLAGEIKVPFVKSFALQRKAGKIIYLLDKTLEECKEEAKSLISIRDNLLGKLFPTLS" }, "paralog": false, "identity": 0.9897260273972602, "score": 583.0, "evalue": 2.17e-213, "description": "Internal stop codon at 766478, 767015. Nonsense mutation around 766478, 767015.", "psc": { "uniref90_id": "UniRef90_P75159", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF638P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75159", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "GIHJHMOALN_1355", "locus": "GCA009939745_01355" }, { "type": "cds", "sequence": "contig_1", "start": 767042, "stop": 767569, "strand": "+", "frame": 2, "gene": "hsdS", "product": "Putative type I specificity subunit S-MpnORF638P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_P75159" ], "nt": "ATGGGTGAAATTAGTTTAGGTGAAGTGTTTCACTTAAAGAGTGGTAAATACTTAAAGGCTGATGAGCGCTTTGAGGATGGCAAATTCCCTTATTATGGTGCTGGTATTGAAAGCACTAGCTTTGTCAATGAACCCAACACCAAAGGTGATACTCTTTCAATGATTGCTAACGGTTACTCCATCGGAAACATTCGCTACCATACTATTCCTTGGTTTAATGGTACTGGTGGTATTGCCATGGAAGCATTAAAACCTAATGAAACCTATGTGCCGTTCTTTTACTGTGCTCTAAAATACATGCAAAAGGATCTAAAGGAACGCTTTAAACGTGATGAATCACCGTTTATTTCCTTAAAACTAGCCGGTGAAATTAAGGTTCCTTTCGTTAAATCGTTTGCACTCCAAAGAAAAGCCGGTAAGATTATTTACTTGTTAGATAAAACTCTTGAAGAATACAAAGAAGAAGCTAAGTCTTTAATCAGTATTCGCGATAACTTATTGGGCAAATTGTTCCCAACACTATCTTAA", "aa": "MGEISLGEVFHLKSGKYLKADERFEDGKFPYYGAGIESTSFVNEPNTKGDTLSMIANGYSIGNIRYHTIPWFNGTGGIAMEALKPNETYVPFFYCALKYMQKDLKERFKRDESPFISLKLAGEIKVPFVKSFALQRKAGKIIYLLDKTLEEYKEEAKSLISIRDNLLGKLFPTLS", "aa_hexdigest": "9dccecccb9e9661e97321afb879f71b4", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "5-prime", "pseudogene": { "start": 766742, "stop": 767569, "observations": { "mutation": [ 767015 ], "stop": [ 767015 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75159", "query_cov": 1.0, "subject_cov": 0.4666666666666667, "identity": 0.9890000000000001, "score": 351.0, "evalue": 1.21e-119, "gene_start": 1, "gene_end": 175, "reference_start": 201, "reference_end": 375, "reference_sequence": "MTPKLKLNTNSNWTKKTLGSLFELKKGEMLEKELLAPDGKYEYFNGGIKASGRTNEFNTFKNTISIIIGGSCGYVRLADKDYFCGQSSCTLTVLDPLEIDLKFAYYALKSQEEKITSLASGTTIKNIRLSDLKDLPIPLVKSIQDQRTIAHALSVFDLRIEHLNELIEVNRKLRDEYAHKLFTLDPDFLTHWNLHELHEQMGEISLGEVFHLKSGKYLKADERFEDGKFPYYGAGIESTSFVNEPNTKGDTLSMIANGYSIGNIRYHTIPWFNGTGGIAMEALKPNKTYVPFFYCALKYMQKDLKERFKRDESPFISLKLAGEIKVPFVKSFALQRKAGKIIYLLDKTLEECKEEAKSLISIRDNLLGKLFPTLS" }, "paralog": false, "identity": 0.9890909090909091, "score": 541.0, "evalue": 9.51e-197, "description": "Internal stop codon at 767015. Nonsense mutation around 767015.", "psc": { "uniref90_id": "UniRef90_P75159", "gene": "HsdS", "product": "Putative type I specificity subunit S.MpnORF638P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75159", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "GIHJHMOALN_1356", "locus": "GCA009939745_01356" }, { "type": "cds", "sequence": "contig_1", "start": 767566, "stop": 767823, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_639", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386E46", "UniRef:UniRef100_P75158", "UniRef:UniRef50_P75158", "UniRef:UniRef90_P75158" ], "nt": "ATGAGTATGGGTCAAAATGAAGCTCTAGAAGCCACTTTTACATATAGTCCAGCTACTCAAGGCATCTTTGGCCGCGCTTCATTTGACCGTTTTACCAGTAACATCAAACTTAACACAAAGTTAAGAATTCAAGTTTCTAGTACGAGTGATCTAATGAAGAAGTTATTGGAGAATAGCTTAACCAGCAGTCTCAAAGACCAGAGCTTTGATAACGAAGGTGTTAACCTTTTCCCTTACACCTTATTTGCTTTGCTTTAA", "aa": "MSMGQNEALEATFTYSPATQGIFGRASFDRFTSNIKLNTKLRIQVSSTSDLMKKLLENSLTSSLKDQSFDNEGVNLFPYTLFALL", "aa_hexdigest": "625b9eba614ac16e3020c78f25fbac3b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386E46", "uniref100_id": "UniRef100_P75158", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386E46", "UniRef:UniRef100_P75158" ] }, "ips": { "uniref100_id": "UniRef100_P75158", "uniref90_id": "UniRef90_P75158" }, "psc": { "uniref90_id": "UniRef90_P75158", "product": "Uncharacterized protein MPN_639", "uniref50_id": "UniRef50_P75158" }, "pscc": { "uniref50_id": "UniRef50_P75158", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75158" ], "product": "Uncharacterized protein MPN_639" }, "genes": [], "id": "GIHJHMOALN_1357", "locus": "GCA009939745_01357" }, { "type": "cds", "sequence": "contig_1", "start": 767826, "stop": 768164, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HS23", "UniRef:UniRef90_A0AB33HS23", "PFAM:PF00938.23" ], "nt": "TTGCTTTCACAGAGAATATTAGAACGTTGGCAAACTGAAGTAGGCCGCTTTGCTAAATCCTTCGGCAACGGTAGTAACCAAAACAGTGTTCAACCCAATGCTACGCTTAAGGGTTTAAAGTTAAGTGAACGTTCAACAACTGAGATTCAATTATTAGCTGAACAAGCCATCACTGTTAGCAAGCAAGAAGTGAAGGAGTTTACCTACAAAGTTGAATCGAATAAGCAATTTGAGGCTACGGTTAAGATTAAGGCAGATTTAAAAATTGATCCAACCAAGGCGATGAGCCATATAGAGAATATTTTTAAGGATGATGAAACTAAGATCAAAATTCTTTAA", "aa": "MLSQRILERWQTEVGRFAKSFGNGSNQNSVQPNATLKGLKLSERSTTEIQLLAEQAITVSKQEVKEFTYKVESNKQFEATVKIKADLKIDPTKAMSHIENIFKDDETKIKIL", "aa_hexdigest": "f2e9b3a3b822c2e98f68abee3234298b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 767826, "stop": 768419, "observations": { "mutation": [ 768176, 768380, 768386 ], "stop": [ 768176, 768380, 768386 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB33HS23", "query_cov": 1.0, "subject_cov": 0.5685279187817259, "identity": 0.991, "score": 207.0, "evalue": 2.7e-66, "gene_start": 1, "gene_end": 112, "reference_start": 86, "reference_end": 197, "reference_sequence": "MLGSMALKLRKWIWASIPSLALILSSCSALVASVELKSLSELQNLADRNTELTKNKRTLITTLRESYGLNPKGTLNLLFEGWRYTLLSQRILERWQTEVGRFAKSFGNGSNQNSVQPNATLKGLKLSERSTTEIQLLAEQAITVSKQEVKEFTYKVESNKQFEATVKIKADLKIDPTKAMSHIENIFKDDETKIKIL" }, "paralog": false, "identity": 0.9847715736040609, "score": 364.0, "evalue": 1.38e-130, "description": "Internal stop codon at 768176, 768380, 768386. Nonsense mutation around 768176, 768380, 768386.", "psc": { "uniref90_id": "UniRef90_A0AB33HS23", "uniref50_id": "UniRef50_A0AB33HS23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB33HS23", "UniRef:UniRef50_A0AB33HS23" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB33HS23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HS23" ] } }, "hypothetical": true, "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 108, "aa_cov": 0.9553571428571429, "hmm_cov": 0.9907407407407407, "evalue": 1.1417389894848319e-35, "score": 118.66890716552734, "start": 2, "stop": 108 } ], "seq_stats": { "molecular_weight": 12757.440999999983, "isoelectric_point": 9.187405586242676 }, "id": "GIHJHMOALN_1358", "locus": "GCA009939745_01358" }, { "type": "cds", "sequence": "contig_1", "start": 768476, "stop": 768856, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75157" ], "nt": "ATGGATGGAAAGATTGATGAATCAAGCAAAGCATTGATTAAATCATTCTTAGAGGGTAATAGTTCGGGAGGTAAAGGTAGCAATGGTAAAAACAGTATTGATGAAAGTGAATACACTGGTGAAAAAGCCAAATTCACTGGTAACTTTATTTTTACCTATACCCCACCAACTGGAGGTGCTAGAAACTTCTCTGATAAATCCTTTGATGTACCAACTAGTTCGATTAACTTTCCCGCTAATGTCAAGATTGATGTAACAACTTCTCACACAAAGCTGAATGAATTATTAGAAAGCAACGAACAGGTCAAAAAGATGAAAAGTCGTCAATTAACAGGTAAGTTATTTAATTTATTACCGTTTTTTTACTACGCTCTGCTTTAA", "aa": "MDGKIDESSKALIKSFLEGNSSGGKGSNGKNSIDESEYTGEKAKFTGNFIFTYTPPTGGARNFSDKSFDVPTSSINFPANVKIDVTTSHTKLNELLESNEQVKKMKSRQLTGKLFNLLPFFYYALL", "aa_hexdigest": "1e19667fd9bf88131ba2f591a06c6b4e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 768427, "stop": 769156, "observations": { "insertion": [ 768499 ], "mutation": [ 768898, 769114 ], "stop": [ 768898, 769114 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75157", "query_cov": 0.9603174603174603, "subject_cov": 0.4033333333333333, "identity": 1.0, "score": 233.0, "evalue": 5.79e-75, "gene_start": 1, "gene_end": 121, "reference_start": 159, "reference_end": 279, "reference_sequence": "MKLKLLLIPLLGSSLLLSACSSATSQVISSLSSAQKYFEANKGELNKKNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLERNLDASRFASAFGSTSKKDDITPNISEKSLFLADTFPGISSEIAKVFKVEKQTVSGFSYSWNSPKKFQVNIQIKMDGKIDESSKALIKSFLEGNSSGGKGSNGKNSIDESEYTGEKAKFTGNFIFTYTPPTGGARNFSDKSFDVPTSSINFPANVKIDVTTSHTKLNELLESNEQVKKMKSRQLTGKLFNLLPFFTTLCFNSFSPFTVFAVIFTIV" }, "paralog": false, "identity": 0.9917355371900827, "score": 464.0, "evalue": 3.64e-168, "description": "Internal stop codon at 768898, 769114. Frameshift due to insertion around 768499. Nonsense mutation around 768898, 769114.", "psc": { "uniref90_id": "UniRef90_P75157", "product": "Uncharacterized lipoprotein MG439 homolog 5", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75157", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "GIHJHMOALN_1359", "locus": "GCA009939745_01359" }, { "type": "cds", "sequence": "contig_1", "start": 768896, "stop": 769099, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGATCAAAAGCTTTTAGAAAGAAATTTAGATGCTTCACGCTTTGCTAGCGCTTTTGGTTCTACTAGCAAGAAGGACGATATTACCCCTAACATTAGTGAAAAAAGTTTATTTTTAGCAGACACTTTTCCTGGTATTAGTTCAGAAATCGCTAAAGTATTTAAAGTTGAAAAGCAAACAGTTAGTGGTTTTTCTTACAGTTGA", "aa": "MDQKLLERNLDASRFASAFGSTSKKDDITPNISEKSLFLADTFPGISSEIAKVFKVEKQTVSGFSYS", "aa_hexdigest": "34706520f715a4c0fd24b91346f9bf69", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 68, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 3.542987794931671e-28, "score": 94.14722442626953, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 7380.2125, "isoelectric_point": 6.140964317321777 }, "id": "GIHJHMOALN_1360", "locus": "GCA009939745_01360" }, { "type": "cds", "sequence": "contig_1", "start": 769112, "stop": 769330, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAAACTTAAACTGTTATTAATTCCTTTATTAGGATCTAGTTTATTGCTGAGCGCTTGTTCCTCTGCAACTAGCCAGGTTATATCGAGTTTAAGTAGCGCGCAAAAATACTTTGAAGCAAATAAAGGTGAATTGAACAAAAAGAACGTTATCAACATTCTGAAAGATGGCTATAACTCTGATCCTAACAAAACAGTTAACGCTCTTTTAGCAGGTTGA", "aa": "MKLKLLLIPLLGSSLLLSACSSATSQVISSLSSAQKYFEANKGELNKKNVINILKDGYNSDPNKTVNALLAG", "aa_hexdigest": "3be1533c2100bb96a1ce5416f96fd50b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.5277777777777778, "hmm_cov": 1.0, "evalue": 1.411816062454621e-12, "score": 43.08498001098633, "start": 35, "stop": 72 } ], "seq_stats": { "molecular_weight": 7637.823099999997, "isoelectric_point": 9.46507091522217 }, "id": "GIHJHMOALN_1361", "locus": "GCA009939745_01361" }, { "type": "cds", "sequence": "contig_1", "start": 769330, "stop": 769497, "strand": "-", "frame": 1, "gene": null, "product": "putative lipo MG440-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniParc:UPI000310140D", "UniRef:UniRef100_P75156", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P75156" ], "nt": "ATGAAGAAAATTAATTTCAATACCACCTTAAAAATAGATATGGTTGGTAAAGACGAAATCATGAAAAAGATTCTTCAAAGTACTACCTTTACTAATAATTTGTCCAGTCAAACTTTCCAAGACCAATCAATTGATCTTTTACCTTACTTGCTTTATTCCATTCTCTAA", "aa": "MKKINFNTTLKIDMVGKDEIMKKILQSTTFTNNLSSQTFQDQSIDLLPYLLYSIL", "aa_hexdigest": "ed0e169656d16334e5d49495f57ca318", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000310140D", "uniref100_id": "UniRef100_P75156", "db_xrefs": [ "SO:0001217", "UniParc:UPI000310140D", "UniRef:UniRef100_P75156" ] }, "ips": { "uniref100_id": "UniRef100_P75156", "uniref90_id": "UniRef90_P75156" }, "psc": { "uniref90_id": "UniRef90_P75156", "product": "Uncharacterized lipoprotein MG440 homolog 3", "uniref50_id": "UniRef50_P47678", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" }, "genes": [], "id": "GIHJHMOALN_1362", "locus": "GCA009939745_01362" }, { "type": "cds", "sequence": "contig_1", "start": 769497, "stop": 770159, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386F78", "UniRef:UniRef100_A0AB38W594", "UniRef:UniRef50_A0AB38W594", "UniRef:UniRef90_A0AB38W594" ], "nt": "ATGAATAAGAAAAGACTATTACCCAAAGCTTCATTAGGTGCTTTATTCATGTTGTTCGGAACAGCTTTAACTGCTTGTTCTAATTCTGATTTTCAAACTAATTTAACAAGTTTGAATCAATTGAGGGAAGGCGTTAATCAAAATACCAGTCTAACCCAAGACAAGAAAGCTTTTGTGGAGTCACTGCAAAAAGCATTTGAAAATAATCCTGAAGGAACTACCAAAGTTTTATTAGACGCTTGGAAATTTACCTTATTAGATTCCAAAATTTTGGAGAGCAAAGACCCTTCTAGATTTGTCAAAGCTTTTGGCTCTGGAAAAAGTAATGAAGATGTTGAGCCTAATGCTAGTGTTAAAGGTTTAAGACTTGATAAACGATTTGAACCAAGCACTGCTAATATTATCAACAATGTTATTTCGCTAAATGAGCAAAAAGTAGAAGCATTTAATATCCAATACAAAAGTCGAACTAGTTTTAAAGTTCAAGTAAAACTTAATGCTCAAGGAAAATACCAAAAAAGTCAAGTTCAATCTTATCTACAACAAATTGGTTTAAATGATGGTGATTTGAAACAAGAAAGTGGCACTCTTTCTGCAGATCTTATTTTTACCTATACTGTACCTGAATCAAATCTTTTCTCTAGAAAGAACTTCGTACTTTAA", "aa": "MNKKRLLPKASLGALFMLFGTALTACSNSDFQTNLTSLNQLREGVNQNTSLTQDKKAFVESLQKAFENNPEGTTKVLLDAWKFTLLDSKILESKDPSRFVKAFGSGKSNEDVEPNASVKGLRLDKRFEPSTANIINNVISLNEQKVEAFNIQYKSRTSFKVQVKLNAQGKYQKSQVQSYLQQIGLNDGDLKQESGTLSADLIFTYTVPESNLFSRKNFVL", "aa_hexdigest": "86861385c155b139ed644e74d99c8666", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002386F78", "uniref100_id": "UniRef100_A0AB38W594", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002386F78", "UniRef:UniRef100_A0AB38W594" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W594", "uniref90_id": "UniRef90_A0AB38W594" }, "psc": { "uniref90_id": "UniRef90_A0AB38W594", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0AB38W594" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W594", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W594" ], "product": "Lipoprotein" }, "genes": [], "id": "GIHJHMOALN_1363", "locus": "GCA009939745_01363" }, { "type": "cds", "sequence": "contig_1", "start": 770162, "stop": 770527, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75155" ], "nt": "ATGGAAGGGATGATTAATGAAAAGAGTAAGGAAACAATTAAGTCTTTTTTGAGTAACAAAGATTCAAATGGAAGTAATAGCGTAGAAGAAAGTGAATATACTGGAGATAAAGCTAAATTCACTGCAGACTTTATCTTTTCCTATACTCCACCAACTGGAGGTACTAGATCTTTAACCGATAAATCATTTGATGTTATCACCAATATGATCAATTTCCCTGCTGATGTAAAGATTGATGTGAACACTTCGCATAATAAACTAAATGAACTGCTTGAGAAAAACGATCAAGTTAAGCGAATGAAAAGTCGTACATTTAATGGAAGAAATATTGACTTGCTACCCTTCTTTTACTATGCTCTACTTTAA", "aa": "MEGMINEKSKETIKSFLSNKDSNGSNSVEESEYTGDKAKFTADFIFSYTPPTGGTRSLTDKSFDVITNMINFPADVKIDVNTSHNKLNELLEKNDQVKRMKSRTFNGRNIDLLPFFYYALL", "aa_hexdigest": "0f6a0c56917a4bae56a36bb06b24bb2e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 770162, "stop": 770827, "observations": { "mutation": [ 770569 ], "stop": [ 770569 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75155", "query_cov": 1.0, "subject_cov": 0.4336917562724014, "identity": 1.0, "score": 234.0, "evalue": 1.22e-75, "gene_start": 1, "gene_end": 121, "reference_start": 159, "reference_end": 279, "reference_sequence": "MKLKLYLIPLLASGIILSACSSTTSQVISSLSSAQKYFEANKGELNKNNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLENNLDPSRFKNTFGPNKNKDDVTPNISEKGLFLNETFTGLGSQIAEVFNVQKQIVSGFSYSWTSPKSFKVNIHIKMEGMINEKSKETIKSFLSNKDSNGSNSVEESEYTGDKAKFTADFIFSYTPPTGGTRSLTDKSFDVITNMINFPADVKIDVNTSHNKLNELLEKNDQVKRMKSRTFNGRNIDLLPFFYYALL" }, "paralog": false, "identity": 0.995475113122172, "score": 441.0, "evalue": 9.46e-160, "description": "Internal stop codon at 770569. Nonsense mutation around 770569.", "psc": { "uniref90_id": "UniRef90_P75155", "product": "Uncharacterized lipoprotein MG439 homolog 4", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75155", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "GIHJHMOALN_1364", "locus": "GCA009939745_01364" }, { "type": "cds", "sequence": "contig_1", "start": 770567, "stop": 771001, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75155" ], "nt": "ATGAAACTTAAACTTTATTTAATTCCTCTTTTAGCTTCCGGCATTATTTTAAGTGCATGTTCTTCCACTACCAGCCAGGTTATATCGAGTTTAAGTAGCGCTCAAAAATACTTTGAAGCAAATAAAGGTGAGTTGAATAAAAATAACGTTATCAACATTCTGAAAGATGGCTATAACTCTGATCCTAACAAAACAGTTAACGCCTTACTAGCAGGCTGGAAGTACACCTTAATGGATCAAAAATTGTTGGAAAATAATTTAGATCCATCTCGTTTTAAAAATACTTTTGGCCCGAATAAAAATAAAGATGACGTTACTCCGAATATTAGCGAGAAAGGTTTATTTTTAAATGAAACTTTTACTGGTTTAGGAAGTCAAATTGCTGAAGTGTTTAATGTACAAAAACAAATTGTTAGTGGTTTTTCTTACAGCTGA", "aa": "MKLKLYLIPLLASGIILSACSSTTSQVISSLSSAQKYFEANKGELNKNNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLENNLDPSRFKNTFGPNKNKDDVTPNISEKGLFLNETFTGLGSQIAEVFNVQKQIVSGFSYS", "aa_hexdigest": "1d0e857d1833acb15c84bc7e24d1f425", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 770264, "stop": 771001, "observations": { "mutation": [ 770569 ], "stop": [ 770569 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75155", "query_cov": 1.0, "subject_cov": 0.5161290322580645, "identity": 1.0, "score": 275.0, "evalue": 1.23e-91, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MKLKLYLIPLLASGIILSACSSTTSQVISSLSSAQKYFEANKGELNKNNVINILKDGYNSDPNKTVNALLAGWKYTLMDQKLLENNLDPSRFKNTFGPNKNKDDVTPNISEKGLFLNETFTGLGSQIAEVFNVQKQIVSGFSYSWTSPKSFKVNIHIKMEGMINEKSKETIKSFLSNKDSNGSNSVEESEYTGDKAKFTADFIFSYTPPTGGTRSLTDKSFDVITNMINFPADVKIDVNTSHNKLNELLEKNDQVKRMKSRTFNGRNIDLLPFFYYALL" }, "paralog": false, "identity": 0.9959183673469387, "score": 482.0, "evalue": 1.69e-175, "description": "Internal stop codon at 770569. Nonsense mutation around 770569.", "psc": { "uniref90_id": "UniRef90_P75155", "product": "Uncharacterized lipoprotein MG439 homolog 4", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75155", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "GIHJHMOALN_1365", "locus": "GCA009939745_01365" }, { "type": "cds", "sequence": "contig_1", "start": 771001, "stop": 771459, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_643", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P75154" ], "nt": "GTGGAAAGATACACACAAGACACTGCCAATATAATTAATAACGTAATTAAACTGGACAAACAAAAAGTAGAAGCTTTTAGCATTCAATACAAAGATCCTAAAAACTTTAGAGTTCAAGTAAAAATTAATGCTAAAGGTAATTACAAAAAGGATACTGTAAAAACTTATTTAAGTCAAGTAGGTTTATCAGATGGTGATCTAAATGATACAGGTACATTAGAAGCTGAAATAATTTACACCTATATGCCACCTGCTGCCAGTTTCTTTTCTGCAAGTAAATTTGACAAACTAACAAGAGCCATCAACTTTAATACCAATTTACGAATACAAATTATAGGCAAAGATAGTGTGATGACTAAATTATTGCAACAAAGTAGTTTTGTAAAGCAACTAGCTGATCAAAAATTTCAAGACCAATCGATAAATCTCTTGCCTTACGTGCTTTACTCCATACTCTAA", "aa": "MERYTQDTANIINNVIKLDKQKVEAFSIQYKDPKNFRVQVKINAKGNYKKDTVKTYLSQVGLSDGDLNDTGTLEAEIIYTYMPPAASFFSASKFDKLTRAINFNTNLRIQIIGKDSVMTKLLQQSSFVKQLADQKFQDQSINLLPYVLYSIL", "aa_hexdigest": "9c3439f16ee6c689de554c12e96f4e75", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 771001, "stop": 771759, "observations": { "mutation": [ 771588 ], "stop": [ 771588 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75154", "query_cov": 1.0, "subject_cov": 0.5033112582781457, "identity": 0.993, "score": 290.0, "evalue": 4.3e-97, "gene_start": 1, "gene_end": 152, "reference_start": 151, "reference_end": 302, "reference_sequence": "MLKKLKVVRLLVNHLIYCPSIFMPYSKNMKKKIWNKTSLGALFMLFGTALTACSNSGFEANLTSLNQLRTSASKNTNLTQNKADLVTALKSAFENNPEGTTRVLLDAWKFTLLDAQILEKQDFSKFSKSFGSGRSIEDVEPSAGVRGLRLVERYTQDTANIINNVIKLDKQKVEAFSIQYKDPKNFRVQVKINAKGNYKKDTVKTYLSQVGLSDGDLNDTGTLEAEIIYTYMPPAASFFSASKFDKLTRAINFNTNLRIQIIGKDSVMTKLLQQSSFVKQLADQKFQDQSINLLPYVLYSIL" }, "paralog": false, "identity": 0.996031746031746, "score": 484.0, "evalue": 9.19e-176, "description": "Internal stop codon at 771588. Nonsense mutation around 771588.", "psc": { "uniref90_id": "UniRef90_P75154", "product": "Uncharacterized protein MPN_643", "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75154", "UniRef:UniRef50_P47678" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" } }, "id": "GIHJHMOALN_1366", "locus": "GCA009939745_01366" }, { "type": "cds", "sequence": "contig_1", "start": 771586, "stop": 771780, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGTTGTTTGGAACAGCTTTAACTGCTTGTTCTAATTCTGGTTTTGAAGCAAATCTTACTAGCTTGAATCAATTAAGAACCAGTGCAAGTAAAAATACTAATTTAACCCAAAATAAAGCTGATTTAGTTACAGCCTTAAAATCAGCTTTTGAAAATAATCCAGAAGGCACTACTAGGGTTTTATTAGACGCTTGA", "aa": "MLFGTALTACSNSGFEANLTSLNQLRTSASKNTNLTQNKADLVTALKSAFENNPEGTTRVLLDA", "aa_hexdigest": "1f85c5aa94dd5ce0d870cf36554211e7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.609375, "hmm_cov": 1.0, "evalue": 6.366118250602301e-11, "score": 37.6708984375, "start": 26, "stop": 64 } ], "seq_stats": { "molecular_weight": 6776.489100000002, "isoelectric_point": 6.036323738098144 }, "id": "GIHJHMOALN_1367", "locus": "GCA009939745_01367" }, { "type": "cds", "sequence": "contig_1", "start": 771830, "stop": 772192, "strand": "-", "frame": 3, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75153" ], "nt": "ATGATGCAAGGCATTGTTGGCAGTGGTGAAGAAAGTAATTCTTTAATTAAATCTTTCTTGAGCTCTGGAAATAATGGCAATGTTACTGAAAATGATTTTAATAATGGAAATGCAAACTTTGATGGCACATTCATATTCACTTTTACTCCGCCTACAGATGGAAGAAGATTTGCATTTAGTAACTTTGATCCAATCACTGGAACAATTAATTTCCCTGCTAATCTTCAAATTGATGCAAGCACAACTCATGAAAAACTCAATATCCTAATGCAAAACAACGAACATGTTAAAAAAATTAAAAGTCGTTCGTTTACTGGTAAATCATTTGATCTATTGCCCTTCTATTTTTATGCCTTACTCTAA", "aa": "MMQGIVGSGEESNSLIKSFLSSGNNGNVTENDFNNGNANFDGTFIFTFTPPTDGRRFAFSNFDPITGTINFPANLQIDASTTHEKLNILMQNNEHVKKIKSRSFTGKSFDLLPFYFYALL", "aa_hexdigest": "fe00e36e5c8379a0bf758de99fde893b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 771830, "stop": 772492, "observations": { "mutation": [ 772231, 772447 ], "stop": [ 772231, 772447 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75153", "query_cov": 1.0, "subject_cov": 0.42402826855123676, "identity": 1.0, "score": 236.0, "evalue": 1.15e-76, "gene_start": 1, "gene_end": 120, "reference_start": 164, "reference_end": 283, "reference_sequence": "MNKKRLLFRTPLDALFLLFGTALSACSSTATNVISSLSSAQKYFDAHKSELIKKNVINLLKEGYSTDSKATVNSLFAGWKYTLMDQKILERNLDASRFTKAFGTNKGKDDVIPSISEKGLFLDETYSGVSQQIAKVLGVQSQKVTGFSYSWSSTTNFKVVISFMMQGIVGSGEESNSLIKSFLSSGNNGNVTENDFNNGNANFDGTFIFTFTPPTDGRRFAFSNFDPITGTINFPANLQIDASTTHEKLNILMQNNEHVKKIKSRSFTGKSFDLLPFYFYALL" }, "paralog": false, "identity": 0.9863636363636363, "score": 429.0, "evalue": 1.1e-154, "description": "Internal stop codon at 772231, 772447. Nonsense mutation around 772231, 772447.", "psc": { "uniref90_id": "UniRef90_P75153", "product": "Uncharacterized lipoprotein MG439 homolog 3", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75153", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "GIHJHMOALN_1368", "locus": "GCA009939745_01368" }, { "type": "cds", "sequence": "contig_1", "start": 772229, "stop": 772432, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGATCAAAAAATACTGGAAAGAAATCTAGATGCTAGTCGCTTTACAAAAGCATTTGGTACTAACAAAGGCAAAGATGATGTCATTCCAAGTATTAGTGAGAAAGGTTTATTTTTAGATGAAACTTACAGTGGTGTAAGTCAACAAATAGTTAAAGTACTTGGAGTGCAAAGCCAAAAAGTAACTGGATTTTCTTACAGCTGA", "aa": "MDQKILERNLDASRFTKAFGTNKGKDDVIPSISEKGLFLDETYSGVSQQIVKVLGVQSQKVTGFSYS", "aa_hexdigest": "45669d012af78d63180a892ddc02fdca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 68, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 9.843388627190943e-30, "score": 99.24095916748047, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 7401.2778000000035, "isoelectric_point": 8.007634162902832 }, "id": "GIHJHMOALN_1369", "locus": "GCA009939745_01369" }, { "type": "cds", "sequence": "contig_1", "start": 772445, "stop": 772681, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAATAAGAAAAGGCTATTATTCAGAACTCCATTAGATGCTTTATTTTTATTGTTCGGCACTGCTTTAAGTGCCTGTTCATCAACTGCCACCAATGTTATTTCTAGTTTAAGTAGTGCGCAAAAATACTTTGATGCTCACAAAAGTGAACTAATCAAAAAGAATGTAATTAATCTTTTAAAAGAAGGTTACAGTACTGATTCAAAAGCTACAGTTAATTCACTTTTTGCTGGTTGA", "aa": "MNKKRLLFRTPLDALFLLFGTALSACSSTATNVISSLSSAQKYFDAHKSELIKKNVINLLKEGYSTDSKATVNSLFAG", "aa_hexdigest": "a27f1dd6ef62585b245e844e16b32470", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.48717948717948717, "hmm_cov": 1.0, "evalue": 5.789423703616081e-10, "score": 34.532745361328125, "start": 41, "stop": 78 } ], "seq_stats": { "molecular_weight": 8487.714099999994, "isoelectric_point": 9.666728019714355 }, "id": "GIHJHMOALN_1370", "locus": "GCA009939745_01370" }, { "type": "cds", "sequence": "contig_1", "start": 772684, "stop": 773058, "strand": "-", "frame": 1, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75152" ], "nt": "ATGAAGGGAAAAGTTGGAAGTGATTCAGAGAGTCAACAATTAATTAAATCTTTTTTAAGTTCTGATAACAATGGATCAAATCAAAATGGCGGAGTAAAAGAAACTGACTTTAATGGTGATTCTGCTAATTTCGATGGTTTCTTTACTTTTACTTATACACCTCCTACTCAAAGTAGAAAATTTGGTGCCACTTCTTTTGACCCTCTTACAACTAAGATTAACTTTCCAGCAGACTTACAAATAGATGTAAGCACTACTCACCAAAAGTTAAATACTTTAATGGAAGCCAATGAACAAGTAAAACAGATTAAAAGCCGTAAGTTTACTGGTAAAACCTTTGACTTGTTACCGTTCTTTTACTACGCACTACTTTAA", "aa": "MKGKVGSDSESQQLIKSFLSSDNNGSNQNGGVKETDFNGDSANFDGFFTFTYTPPTQSRKFGATSFDPLTTKINFPADLQIDVSTTHQKLNTLMEANEQVKQIKSRKFTGKTFDLLPFFYYALL", "aa_hexdigest": "418b1dd1c030be37cf8acbe8ca0300f6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 772684, "stop": 773358, "observations": { "mutation": [ 773100, 773313 ], "stop": [ 773100, 773313 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75152", "query_cov": 1.0, "subject_cov": 0.4381625441696113, "identity": 1.0, "score": 241.0, "evalue": 2.91e-78, "gene_start": 1, "gene_end": 124, "reference_start": 160, "reference_end": 283, "reference_sequence": "MKLKLKFLLISLLGSSLLLSACSSAATQVISSLSSAQKYFESSQGELNKKNVIKILKEGYESDANKAVHALLAGWKYTLMDQQLLSKEVDSRFIKAFGSGRDKGDVTPSVSEKGLYLNETYTGFSSQIAKVLGVQSQTVKQFNYKWSSNSDFKVQIQISMKGKVGSDSESQQLIKSFLSSDNNGSNQNGGVKETDFNGDSANFDGFFTFTYTPPTQSRKFGATSFDPLTTKINFPADLQIDVSTTHQKLNTLMEANEQVKQIKSRKFTGKTFDLLPFFYYALL" }, "paralog": false, "identity": 0.9910714285714286, "score": 437.0, "evalue": 5.79e-158, "description": "Internal stop codon at 773100, 773313. Nonsense mutation around 773100, 773313.", "psc": { "uniref90_id": "UniRef90_P75152", "product": "Uncharacterized lipoprotein MG439 homolog 2", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75152", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "GIHJHMOALN_1371", "locus": "GCA009939745_01371" }, { "type": "cds", "sequence": "contig_1", "start": 773098, "stop": 773298, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGACCAGCAATTACTTTCTAAAGAAGTTGATAGTCGCTTTATCAAAGCTTTTGGTAGCGGACGCGATAAAGGCGATGTAACTCCTAGTGTTAGTGAAAAGGGATTGTACTTAAACGAAACTTACACTGGCTTTAGTAGTCAAATTGCCAAAGTCTTAGGTGTACAAAGTCAAACTGTTAAACAATTTAATTACAAGTGA", "aa": "MDQQLLSKEVDSRFIKAFGSGRDKGDVTPSVSEKGLYLNETYTGFSSQIAKVLGVQSQTVKQFNYK", "aa_hexdigest": "6b02599e548b92439bb16ad32249be61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 68, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 9.434004752821307e-28, "score": 92.75507354736328, "start": 1, "stop": 66 } ], "seq_stats": { "molecular_weight": 7351.177600000002, "isoelectric_point": 9.04899196624756 }, "id": "GIHJHMOALN_1372", "locus": "GCA009939745_01372" }, { "type": "cds", "sequence": "contig_1", "start": 773311, "stop": 773535, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAAACTGAAACTTAAATTTCTATTAATTCCTCTTTTAGGTTCTAGTTTGTTGTTAAGCGCTTGTTCTTCAGCAGCTACTCAAGTAATTTCTAGTTTAAGTAGTGCTCAAAAATACTTTGAATCCAGTCAAGGTGAACTCAACAAAAAGAATGTCATCAAAATCTTAAAGGAAGGTTATGAATCTGATGCTAATAAGGCAGTCCATGCTTTGCTAGCTGGTTGA", "aa": "MKLKLKFLLIPLLGSSLLLSACSSAATQVISSLSSAQKYFESSQGELNKKNVIKILKEGYESDANKAVHALLAG", "aa_hexdigest": "1c78d29c554ad9396126998d01590024", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.5135135135135135, "hmm_cov": 1.0, "evalue": 2.589585454991122e-10, "score": 35.676395416259766, "start": 37, "stop": 74 } ], "seq_stats": { "molecular_weight": 7896.181899999997, "isoelectric_point": 9.41762218475342 }, "id": "GIHJHMOALN_1373", "locus": "GCA009939745_01373" }, { "type": "cds", "sequence": "contig_1", "start": 773535, "stop": 774098, "strand": "-", "frame": 2, "gene": null, "product": "putative lipo MG440-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P75151" ], "nt": "TTGGAAAAACAAGATCCAGTTAAATTTGTTAAAGCATTTGGTAGCGGTAAAAGTAAGGAAGACATAGAACCTAGTGCTGGCGTACGTGGTTTAAGATTTGTTGAACGTTTTGGTGATAACACAGCTAGTCTTATTAAAAATGCAATTTTATTGGATCAACAAAAAGTAGAGGTTTTTAATATTCGCTTTAAAAGTAGTCGTGATTTCACTATTCAGATTAAGCTGAATGCAAAAGGTAATTACAAAAAGAGTGAAGTCGAGAAATATAAGTCACAAATTGGTCTTCAAGATAATGAACTGGGCGATAAGGAAGAAGGCACTTTAGAAGCTGATTTAATCTTCACTTACACTCCACCTGCTTCAAATTTGTTTTCTAAGAGTAATTTTGACAAGTTAACTAAATCGATTAATTTCAATACCAATCTGAAATTAGAAATGGTTGGTAAAGACGAAGTAATGAGAAAGATTCTTAACAGTAGTGCCTTCACTAATAATCTTGCCAGTGTTAATTACCCAAACCAAGCAGTTAATTTACTGCCTTACGTACTTTACTCCATCCTCTAA", "aa": "MEKQDPVKFVKAFGSGKSKEDIEPSAGVRGLRFVERFGDNTASLIKNAILLDQQKVEVFNIRFKSSRDFTIQIKLNAKGNYKKSEVEKYKSQIGLQDNELGDKEEGTLEADLIFTYTPPASNLFSKSNFDKLTKSINFNTNLKLEMVGKDEVMRKILNSSAFTNNLASVNYPNQAVNLLPYVLYSIL", "aa_hexdigest": "e027f7d16dff0dcbebeacd5c60a4ebf1", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 773535, "stop": 774368, "observations": { "mutation": [ 774128, 774350 ], "stop": [ 774128, 774350 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75151", "query_cov": 1.0, "subject_cov": 0.6750902527075813, "identity": 0.9890000000000001, "score": 356.0, "evalue": 1.06e-122, "gene_start": 1, "gene_end": 187, "reference_start": 91, "reference_end": 277, "reference_sequence": "MNKKSIWSKTAFGSLFLLLGTAFTACSNYDFQANLTSLNQLRTSANKDINLTQDKNTLIDALKTAFENNSEGTTKVLLDAWKFSLYDAKILEKQDPVKFVKAFGSGKSKEDIEPSAGVRGLRFVERFGDNTASLIKNAILLDQQKVEVFNIRFKSSRDFTIQIKLNAKGNYKKSEVEKYKSQIGVQDNELGDKEEGTLEADLIFTYTPPASNLFSKSNFDKLTKSINFNTNLKLEMVGKDEVMRKILNSSAFTNNLASVNYPNQAVNLLPYVLYSIL" }, "paralog": false, "identity": 0.9891696750902527, "score": 530.0, "evalue": 1.09e-193, "description": "Internal stop codon at 774128, 774350. Nonsense mutation around 774128, 774350.", "psc": { "uniref90_id": "UniRef90_P75151", "product": "Uncharacterized lipoprotein MG440 homolog 1", "uniref50_id": "UniRef50_P47678", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75151", "UniRef:UniRef50_P47678", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" } }, "id": "GIHJHMOALN_1374", "locus": "GCA009939745_01374" }, { "type": "cds", "sequence": "contig_1", "start": 774126, "stop": 774326, "strand": "-", "frame": 2, "gene": null, "product": "lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912A3C4", "UniRef:UniRef90_UPI001912A3C4" ], "nt": "TTGTTTCTTTTATTAGGAACAGCGTTTACTGCTTGTTCTAACTATGACTTTCAAGCTAACTTAACTAGTTTGAACCAATTGAGAACTTCAGCCAATAAAGACATTAATTTAACTCAGGACAAAAATACCTTAATTGATGCTTTAAAAACTGCCTTTGAAAATAATTCAGAAGGTACCACTAAGGTTTTACTAGATGCCTGA", "aa": "MFLLLGTAFTACSNYDFQANLTSLNQLRTSANKDINLTQDKNTLIDALKTAFENNSEGTTKVLLDA", "aa_hexdigest": "c535947d0bfa283cab62c1a492dee459", "start_type": "TTG", "rbs_motif": "AAAA", "genes": [], "truncated": "both", "pseudogene": { "start": 774048, "stop": 774368, "observations": { "mutation": [ 774128, 774350 ], "stop": [ 774128, 774350 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001912A3C4", "query_cov": 1.0, "subject_cov": 0.6226415094339622, "identity": 0.985, "score": 124.0, "evalue": 1.27e-35, "gene_start": 1, "gene_end": 66, "reference_start": 15, "reference_end": 80, "reference_sequence": "MNKKSIWSKTAFGSLFLLLGTAFTACSNYDFQANLTSLNQLRTSANKDINLTQDKNTLIDALKTAFENNSEGTTKVLLDAWKFSLYDAKILEKQDRVKFVKAFGRG" }, "paralog": false, "identity": 0.9622641509433962, "score": 196.0, "evalue": 2.95e-66, "description": "Internal stop codon at 774128, 774350. Nonsense mutation around 774128, 774350.", "psc": { "uniref90_id": "UniRef90_UPI001912A3C4", "product": "lipoprotein", "uniref50_id": "UniRef50_UPI001912A3C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001912A3C4", "UniRef:UniRef50_UPI001912A3C4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001912A3C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001912A3C4" ], "product": "lipoprotein" } }, "id": "GIHJHMOALN_1375", "locus": "GCA009939745_01375" }, { "type": "cds", "sequence": "contig_1", "start": 774391, "stop": 774774, "strand": "-", "frame": 1, "gene": null, "product": "putative lipo MG439-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P75150" ], "nt": "ATGAAAGGAAAAGTTGGTAGTGATGGCACAAGTCAAACTTTAATTAAGTCTTTTTTAGGTACTGATAGCAAAGGCAGTGGAAGCAATAATCAAAATGGTGGTGTAACTGAAAAAGATTTTGAAGGTGACCAAGCTAACTTTGATGGCAATTTTATCTTTACTTACACTCAACCTTCCGATGGCAGAAGACTTGCGTCCAGCAACTTTGATCCAATTACTGGAACCATCAATTTTCCTGCTGATCTCCAAATTGAGGTAAGTACTAGTCATGAAAAGTTAAACACTTTAATGACAACTAATACTCAGGTAGGAATGATTAAGAACCGCTCTTTTAAAGGCAAATCATTTAATCTTTTGCCTTTTTTTTACTACGCCCTTCTCTAA", "aa": "MKGKVGSDGTSQTLIKSFLGTDSKGSGSNNQNGGVTEKDFEGDQANFDGNFIFTYTQPSDGRRLASSNFDPITGTINFPADLQIEVSTSHEKLNTLMTTNTQVGMIKNRSFKGKSFNLLPFFYYALL", "aa_hexdigest": "4318a218e250f7d97a8026f9bc23c215", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 774384, "stop": 775074, "observations": { "insertion": [ 774414 ], "mutation": [ 774816, 775029 ], "stop": [ 774816, 775029 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75150", "query_cov": 0.9606299212598425, "subject_cov": 0.4178082191780822, "identity": 1.0, "score": 239.0, "evalue": 1.76e-77, "gene_start": 1, "gene_end": 122, "reference_start": 164, "reference_end": 285, "reference_sequence": "MNKKSILSKTSLGSLFFLFGTALSACSSATTEVISSFSSAQKYFSANKKELNKRNLVTILKDSYNSDPKSTVNSLLAGWKYSLLDQKLLENPMDPSRFSKAFGSNTKDDVTPNISEKGLYLAETYPGVSSQIAQVLGVQSQKVTGFSYSWTSKTKFEVKILIKMKGKVGSDGTSQTLIKSFLGTDSKGSGSNNQNGGVTEKDFEGDQANFDGNFIFTYTQPSDGRRLASSNFDPITGTINFPADLQIEVSTSHEKLNTLMTTNTQVGMIKNRSFKGKSFNLLPFFTTPFSNF" }, "paralog": false, "identity": 0.9912663755458515, "score": 443.0, "evalue": 3.3e-160, "description": "Internal stop codon at 774816, 775029. Frameshift due to insertion around 774414. Nonsense mutation around 774816, 775029.", "psc": { "uniref90_id": "UniRef90_P75150", "product": "Uncharacterized lipoprotein MG439 homolog 1", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75150", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "GIHJHMOALN_1376", "locus": "GCA009939745_01376" }, { "type": "cds", "sequence": "contig_1", "start": 774814, "stop": 775017, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "TTGCTGGATCAAAAGTTATTGGAGAACCCAATGGATCCTTCCCGTTTTTCTAAAGCTTTTGGTTCTAACACTAAAGATGATGTTACCCCGAACATCAGTGAAAAAGGTTTATATCTTGCAGAAACTTATCCCGGTGTAAGTAGTCAAATCGCTCAAGTTCTTGGTGTCCAAAGCCAAAAGGTAACTGGTTTTTCTTATAGCTGA", "aa": "MLDQKLLENPMDPSRFSKAFGSNTKDDVTPNISEKGLYLAETYPGVSSQIAQVLGVQSQKVTGFSYS", "aa_hexdigest": "5a87f02c6a0b1acb82b95c677be11f7c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 69, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.6204136213812302e-29, "score": 98.53238677978516, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 7315.119000000002, "isoelectric_point": 5.042607688903809 }, "id": "GIHJHMOALN_1377", "locus": "GCA009939745_01377" }, { "type": "cds", "sequence": "contig_1", "start": 775027, "stop": 775263, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAACAAAAAATCGATTTTGTCAAAGACCAGTCTAGGTTCTTTGTTTTTCCTGTTTGGCACAGCTTTAAGTGCCTGCTCCTCTGCCACCACAGAAGTAATTTCCAGTTTTAGTAGTGCCCAGAAGTACTTTAGTGCCAACAAAAAAGAACTTAATAAACGTAATCTAGTAACCATTTTGAAGGATAGTTATAATTCTGATCCCAAGTCAACAGTTAATTCTTTATTAGCTGGCTGA", "aa": "MNKKSILSKTSLGSLFFLFGTALSACSSATTEVISSFSSAQKYFSANKKELNKRNLVTILKDSYNSDPKSTVNSLLAG", "aa_hexdigest": "40b50d430d2f5d5994d1915b01417aac", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.48717948717948717, "hmm_cov": 1.0, "evalue": 5.927172863892566e-12, "score": 41.045589447021484, "start": 41, "stop": 78 } ], "seq_stats": { "molecular_weight": 8388.492099999996, "isoelectric_point": 9.78032131195068 }, "id": "GIHJHMOALN_1378", "locus": "GCA009939745_01378" }, { "type": "cds", "sequence": "contig_1", "start": 775620, "stop": 775784, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGCCAATTTAAAGCAAATGTACTAGCTATCTTACTAGTCTCCTTGTTTTTAATTAACGGTCTTGTTTTTCTTTCGAAAACATTATTTAAGTTATTTAATTTTCGTGTCACCCACTCATTAAATAACTACTATACCTTTAATCTGTTAATTAAAGCTTGA", "aa": "MKSQFKANVLAILLVSLFLINGLVFLSKTLFKLFNFRVTHSLNNYYTFNLLIKA", "aa_hexdigest": "7b062f5e42e9a4aca979e4c01b7d1285", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6268.4995, "isoelectric_point": 10.390772438049318 }, "id": "GIHJHMOALN_1379", "locus": "GCA009939745_01379" }, { "type": "cds", "sequence": "contig_1", "start": 776671, "stop": 777081, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_649", "db_xrefs": [ "RefSeq:WP_010875006.1", "SO:0001217", "UniParc:UPI000013AF9D", "UniRef:UniRef100_P75148", "UniRef:UniRef50_P75148", "UniRef:UniRef90_P75148" ], "nt": "TTGAAAATTTCCTTAAGCACTAGTCTCTTTAGTATCGAACAAAAGGTTGAGTATTTCAATTTGAATTACCAAAGCCTTAGTGATTTTTCAGTTGTTGCCAAATTGAACTATACCTTTACATGGTATGGCAATGATTTTTCAATAGGTTTCGCTCCAAAAAAAGGTGAAAAGCTTTATTTTGATCTCTTTTTTACTTTTAAAGCTTCTCCCAACCATCCATTTGCAGCAGAAAACTTTAAACCTGATTCAGAAGCAATCGACTTTTATGTTTCATTTAGCTGGAGATTAGAAGGAAAAGACGAAGTTAGCAAAAAACTTTTTGAATTAAGTGTTTTTGGTAGAGCAAGAGCTTTTCAAATTGATGACTACAAAATCAACCTTTTTAGTTATTTAGTTTACGTAATTAGATAA", "aa": "MKISLSTSLFSIEQKVEYFNLNYQSLSDFSVVAKLNYTFTWYGNDFSIGFAPKKGEKLYFDLFFTFKASPNHPFAAENFKPDSEAIDFYVSFSWRLEGKDEVSKKLFELSVFGRARAFQIDDYKINLFSYLVYVIR", "aa_hexdigest": "74f3664669cc55e687dbc7fd4ab2c752", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AF9D", "ncbi_nrp_id": "WP_010875006.1", "uniref100_id": "UniRef100_P75148", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AF9D", "RefSeq:WP_010875006.1", "UniRef:UniRef100_P75148" ] }, "ips": { "uniref100_id": "UniRef100_P75148", "uniref90_id": "UniRef90_P75148" }, "psc": { "uniref90_id": "UniRef90_P75148", "product": "Uncharacterized protein MPN_649", "uniref50_id": "UniRef50_P75148" }, "pscc": { "uniref50_id": "UniRef50_P75148", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75148" ], "product": "Uncharacterized protein MPN_649" }, "genes": [], "id": "GIHJHMOALN_1380", "locus": "GCA009939745_01380" }, { "type": "cds", "sequence": "contig_1", "start": 777087, "stop": 777371, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_650", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75147", "UniRef:UniRef90_P75147" ], "nt": "ATGAAATTTAAATATCTATCAACTCCGTTATTATTTTCAGCTTTATTATTTAGCGCTTGTTCTAGCGTCAATGTTGGAGCTAATTTGAAAAGTCTTATTAAGGAAACCACCGATCAAGACCTTGATTTGTCTGAATCAGTTAGTACTTCTGAGGGAAAGAAGAATTTAATAAGTTCTTTAAAGAAGTCTTACGAAACTAACCCTAGCAAAACAGCTAGTGTACTCTTGAATGCCTGGAAGCAAAGTGTAGAAAACGGTCAAATAGAGAGTTCTGAAACTAATTGA", "aa": "MKFKYLSTPLLFSALLFSACSSVNVGANLKSLIKETTDQDLDLSESVSTSEGKKNLISSLKKSYETNPSKTASVLLNAWKQSVENGQIESSETN", "aa_hexdigest": "856656c27a4a32982dfc34ad2685c8b5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75147", "query_cov": 1.0, "subject_cov": 0.9306930693069307, "identity": 1.0, "score": 167.0, "evalue": 2.92e-52, "valid": true, "product": "Uncharacterized lipoprotein MPN_650", "uniref50_id": "UniRef50_P75147", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75147", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75147" ], "product": "Uncharacterized lipoprotein MPN_650" }, "genes": [], "id": "GIHJHMOALN_1381", "locus": "GCA009939745_01381" }, { "type": "cds", "sequence": "contig_1", "start": 777371, "stop": 777508, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036F3933", "UniRef:UniRef100_A0AB38W7G3", "UniRef:UniRef50_A0AB38W7G3", "UniRef:UniRef90_A0AB38W7G3" ], "nt": "ATGAGACTAGAAGTTTTTCCTAAGGATGAAATAACCAAAAAACTTTTAGATAATGGTTCTATTGGAAAATTGGTAGATAAAATTTACTTTGATTACAAAGTAAATTTACTGCTATATCTGGTTTATTCAGTCCTCTAA", "aa": "MRLEVFPKDEITKKLLDNGSIGKLVDKIYFDYKVNLLLYLVYSVL", "aa_hexdigest": "b33f894509a27877cd9987bb4301d6d1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00036F3933", "uniref100_id": "UniRef100_A0AB38W7G3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036F3933", "UniRef:UniRef100_A0AB38W7G3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W7G3", "uniref90_id": "UniRef90_A0AB38W7G3" }, "psc": { "uniref90_id": "UniRef90_A0AB38W7G3", "uniref50_id": "UniRef50_A0AB38W7G3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W7G3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W7G3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5308.276500000001, "isoelectric_point": 7.982878303527833 }, "id": "GIHJHMOALN_1382", "locus": "GCA009939745_01382" }, { "type": "cds", "sequence": "contig_1", "start": 777533, "stop": 777766, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5Y3", "UniRef:UniRef90_A0AB38W5Y3" ], "nt": "TTGTTTGACATTCAGGCAAACAATAAAAATGATTTTAAGGTTGTTGTTAAAATGAACACAACTTTTAATGCTGATATTAATCAAAAACCAAATCAAGGAGGGTTAATAATGTCGGGCTTTGGCAACTACTTGCTTAATTTTGATCTTATTTACACATACAAACCATTTAAAAACAAACCAATTCTAGAGACAAGTATTGTTAAAAACTCTGATAATAAATTAGGGAATTTGTGA", "aa": "MFDIQANNKNDFKVVVKMNTTFNADINQKPNQGGLIMSGFGNYLLNFDLIYTYKPFKNKPILETSIVKNSDNKLGNL", "aa_hexdigest": "639af4a3159d0034284dc2ca5fc6b675", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W5Y3", "query_cov": 1.0, "subject_cov": 0.927710843373494, "identity": 1.0, "score": 156.0, "evalue": 2.21e-48, "valid": true, "uniref50_id": "UniRef50_A0AB38W5Y3" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5Y3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8761.966999999995, "isoelectric_point": 9.302352714538571 }, "id": "GIHJHMOALN_1383", "locus": "GCA009939745_01383" }, { "type": "cds", "sequence": "contig_1", "start": 778287, "stop": 778439, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E56736E", "UniRef:UniRef90_UPI001E56736E" ], "nt": "GTGATTGCTGTCATTATTGCCATTGCTGTAATAGCTGCTGGTCAAACCGAAGCATTTACAAAAATTGTTGGTCAAAAATCAGTAATGTTTTTATGTGTGATGATATTCGTCCCAATTGCAGCGCTAATCTTAAAACACACTGAAAAGTTTTGA", "aa": "MIAVIIAIAVIAAGQTEAFTKIVGQKSVMFLCVMIFVPIAALILKHTEKF", "aa_hexdigest": "1524574f7646eb52f194d46a2ff77716", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 778287, "stop": 778499, "observations": { "mutation": [ 778437 ], "stop": [ 778437 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E56736E", "query_cov": 1.0, "subject_cov": 0.7142857142857143, "identity": 0.96, "score": 84.0, "evalue": 2.39e-20, "gene_start": 1, "gene_end": 50, "reference_start": 1, "reference_end": 50, "reference_sequence": "MIAVIIAIAVIAAGQTEAFTKIVGQKSVMFLGVMIFGPIAALILKHTEKFWIHRIKSGFEMLVNNFYLAF" }, "paralog": false, "identity": 0.9285714285714286, "score": 122.0, "evalue": 4.17e-38, "description": "Internal stop codon at 778437. Nonsense mutation around 778437.", "psc": { "uniref90_id": "UniRef90_UPI001E56736E", "uniref50_id": "UniRef50_UPI001E56736E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E56736E", "UniRef:UniRef50_UPI001E56736E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E56736E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E56736E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5390.663499999998, "isoelectric_point": 9.102758598327636 }, "id": "GIHJHMOALN_1384", "locus": "GCA009939745_01384" }, { "type": "cds", "sequence": "contig_1", "start": 778467, "stop": 779510, "strand": "+", "frame": 3, "gene": null, "product": "PTS mannitol transporter subunit IICB", "db_xrefs": [ "EC:2.7.1.197", "KEGG:K02800", "RefSeq:WP_235028915.1", "SO:0001217", "UniParc:UPI001F433E99", "UniRef:UniRef100_UPI001F2174FA", "UniRef:UniRef50_UPI001F2174FA", "UniRef:UniRef90_UPI001F2174FA" ], "nt": "ATGCTGGTAAATAATTTTTACCTTGGCTTTTTAGGTTTCGCCTTAATATTTCCTAGTTTTTACCTATCAATTTACTTAATTGGTTACATTCAATTAGGTTTAAAACTGCTTGTTGAAATAATGCAACAGTACAAACTTTATCCAATAGCTGCCATTGTGATAGAGCCGGCCAAAGTTTTATTCCTAAATAATGCCATTAACCATGGCGTGCTAACTCCATTAGGCTTACAACAAGTAAGAGACAGTGGTAAATCAATTCTTTTCTTATTGGAATCAAATCCTGGTCCAGGTTTGGGTCTCCTTGTTGCTTTCTTAATCTTTTTCTTTAAACGTGATAAAAAGCTTTCTAGTAATGCGGCATCTTCTGGTCCTATTCACCTCTTTGGTGGTATCCATGAAGTTTACTTTCCGTTTGTGCTATTAAAACCTGTGTTAATTCTGGCCACAATTGCTGGTGGTGTTGTGGGAAACGGCATTTTGCAAATATTTAATGCTGGGACAATTGCACCAGTGTCACCTGGTTCTGTTATTGCTGGTTTTCTCCAAATTAATAAAACACCTTTAGATGTAGCTGGTTATGCTTTGGCACTCGTTTTATCAGCTGTAACTTCGTTGTTAATTTCGCTTTTATTACTTTCTTTAACACGTAAAAAACAATTGAAAACACTGCAAGAAGCGCAAGCACAAGTTGCAGAAATGAAGCAAACGCCTGCAAAAAAACCAAGACAAAAAGATACTCCAGCAATTGCTACAAAGATAGACTTTTCCCAAGTTACCTTTGTTTGCGATGCAGGTATGGGTTCTTCTACTATGGGAGCAGCTATATTTAGAAAAGAACTAAAAAATCAAAATATTGAAGACATTACAGTGATTAATAAAGCAATAGTTGATTTAAAAGATGAAAAGGTAATTATTACCATTTCCCAACTTTATGATCGCGTAAAAGCGAAGCGTGCAGACGCTACGATTTATACCATCAATCAGTTCTTAGATAAACAAGGCTATTTAACTATTATTGAAAAGATTAAAAATGAAAAGAATTAA", "aa": "MLVNNFYLGFLGFALIFPSFYLSIYLIGYIQLGLKLLVEIMQQYKLYPIAAIVIEPAKVLFLNNAINHGVLTPLGLQQVRDSGKSILFLLESNPGPGLGLLVAFLIFFFKRDKKLSSNAASSGPIHLFGGIHEVYFPFVLLKPVLILATIAGGVVGNGILQIFNAGTIAPVSPGSVIAGFLQINKTPLDVAGYALALVLSAVTSLLISLLLLSLTRKKQLKTLQEAQAQVAEMKQTPAKKPRQKDTPAIATKIDFSQVTFVCDAGMGSSTMGAAIFRKELKNQNIEDITVINKAIVDLKDEKVIITISQLYDRVKAKRADATIYTINQFLDKQGYLTIIEKIKNEKN", "aa_hexdigest": "75a632c8ec0e9d3f847cef5f56bc99c0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001F433E99", "ncbi_nrp_id": "WP_235028915.1", "uniref100_id": "UniRef100_UPI001F2174FA", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F433E99", "RefSeq:WP_235028915.1", "UniRef:UniRef100_UPI001F2174FA" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F2174FA", "uniref90_id": "UniRef90_UPI001F2174FA" }, "psc": { "uniref90_id": "UniRef90_UPI001F2174FA", "product": "PTS mannitol transporter subunit IICB", "ec_ids": [ "2.7.1.197" ], "uniref50_id": "UniRef50_UPI001F2174FA", "kegg_orthology_id": "K02800" }, "pscc": { "uniref50_id": "UniRef50_UPI001F2174FA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2174FA" ], "product": "PTS mannitol transporter subunit IICB" }, "genes": [], "id": "GIHJHMOALN_1385", "locus": "GCA009939745_01385" }, { "type": "cds", "sequence": "contig_1", "start": 779497, "stop": 779700, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01232.29" ], "nt": "ATGAAAAGAATTAATGTATTGCACTTTGGCGCTGGTAATATAGGTAGAGGTGTTATATTACCGATTTACCAACAAAATGACTTTTCCATTGATTTAGTGGAATTGAATCAAAATACAGTTAATGAGTTACAAAAGCAAAAACAATACCAAGTTCATTACCTTGATTGTGATCAAAGTCAGTTAGTTAATGACTTTAATACTTGA", "aa": "MKRINVLHFGAGNIGRGVILPIYQQNDFSIDLVELNQNTVNELQKQKQYQVHYLDCDQSQLVNDFNT", "aa_hexdigest": "ddda0e9fd35972435ab7f37929199393", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01232.29", "name": "Mannitol_dh", "length": 62, "aa_cov": 0.9104477611940298, "hmm_cov": 1.0, "evalue": 1.0604355884499946e-21, "score": 73.26538848876953, "start": 5, "stop": 65 } ], "seq_stats": { "molecular_weight": 7779.6486, "isoelectric_point": 5.393133735656738 }, "id": "GIHJHMOALN_1386", "locus": "GCA009939745_01386" }, { "type": "cds", "sequence": "contig_1", "start": 779734, "stop": 780504, "strand": "+", "frame": 1, "gene": "mtlD", "product": "mannitol-1-phosphate 5-dehydrogenase", "db_xrefs": [ "COG:COG0246", "COG:G", "EC:1.1.1.17", "GO:0005829", "GO:0008926", "GO:0019592", "KEGG:K00009", "SO:0001217", "UniRef:UniRef50_P78008", "UniRef:UniRef90_P78008" ], "nt": "ATGGAAAGAGCTGATGTTATTTCCACTTCAATAGGTGCTAAAAACTTAGCTAGTTTAAAAACTTTGTTTGACAAAGCTAAGTTCCATAAAAGGGCTATAGTGCTTTGCTTTGAAAACGGCTTTCGGATTAGTTCTAACTTTAAAAACATTTTGCAATTAAATAACACACAAGTAAATTTTGTGGATGTGGTAATCGATACAATTGCACCTAACTTCGAAAAGAAAGCGAATTTCTTGGATATTTATTGCGAGAAGTATTCAGAAATTTATGCTGAAACTTTCCCGCTAGAAATTAAGGGAGTAAATCAAACGAATAGTTTAGATAGATTCATTATTAAAAAACTTTTATTAGTCAATGCACTGCACAGTGTTATTGGACTTTTAGGCTTTCAACAAAAGCTTAAGTATGTACATGAAACATTGCAAGTTAAAAGTAATTTGACTTTTGTAGAAAAATTAGCCCAACAAATTATTGATGCCCTTTGTGCTGAATATCCTGAATTTAATAAAAATAATTTGTTAAGTTATGGCAAAAATAACTTAGTCCGTTTTGCTAATCCCAAGATTCAAGATTTAAACACACGCTTAATTAGGGAACCATTACGTAAACTTAACCAAAATGAACGCTTTTATGCCATTTATAAACTCTTTAAAAAGAATAAGATTGCACTAAATAATATTTTGCAAGTTTATCTGATGGTTTTAAAAACTAATATAACAGATGATACCGAAAGCCAACAAATTGCTAAATTAATAAATGAAAAAGCTTGA", "aa": "MERADVISTSIGAKNLASLKTLFDKAKFHKRAIVLCFENGFRISSNFKNILQLNNTQVNFVDVVIDTIAPNFEKKANFLDIYCEKYSEIYAETFPLEIKGVNQTNSLDRFIIKKLLLVNALHSVIGLLGFQQKLKYVHETLQVKSNLTFVEKLAQQIIDALCAEYPEFNKNNLLSYGKNNLVRFANPKIQDLNTRLIREPLRKLNQNERFYAIYKLFKKNKIALNNILQVYLMVLKTNITDDTESQQIAKLINEKA", "aa_hexdigest": "72981174081e762d6173b285a6177393", "start_type": "ATG", "rbs_motif": null, "genes": [ "mtlD" ], "truncated": "both", "pseudogene": { "start": 779497, "stop": 780591, "observations": { "mutation": [ 779698, 780502, 780544 ], "stop": [ 779698, 780502, 780544 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P78008", "query_cov": 1.0, "subject_cov": 0.7032967032967034, "identity": 0.996, "score": 493.0, "evalue": 3.34e-174, "gene_start": 1, "gene_end": 256, "reference_start": 80, "reference_end": 335, "reference_sequence": "MKRINVLHFGAGNIGRGVILPIYQQNDFSIDLVELNQNTVNELQKQKQYQVHYLDCDQSQLVNDFNTWNLKDEAKIIELMERADVISTSIGAKNLASLKTLFDKAKFHKRAIVLCFENGFRISSNFKNILQLNNTQVNFVDVVIDTIAPNFEKKANFLDIYCEKYSEIYAETFPLEIKGVNQKNSLDRFIIKKLLLVNALHSVIGLLGFQQKLKYVHETLQVKSNLTFVEKLAQQIIDALCAEYPEFNKNNLLSYGKNNLVRFANPKIQDLNTRLIREPLRKLNQNERFYAIYKLFKKNKIALNNILQVYLMVLKTNITDDTESQQIAKLINEKAWTELAKLSSLEESEWNLIKQELSREITKK" }, "paralog": false, "identity": 0.989010989010989, "score": 697.0, "evalue": 3.29e-257, "description": "Internal stop codon at 779698, 780502, 780544. Nonsense mutation around 779698, 780502, 780544.", "psc": { "uniref90_id": "UniRef90_P78008", "gene": "mtlD", "product": "mannitol-1-phosphate 5-dehydrogenase", "ec_ids": [ "1.1.1.17" ], "uniref50_id": "UniRef50_P78008", "cog_id": "COG0246", "cog_category": "G", "kegg_orthology_id": "K00009", "go_ids": [ "GO:0005829", "GO:0008926", "GO:0019592" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P78008", "EC:1.1.1.17", "UniRef:UniRef50_P78008", "COG:COG0246", "COG:G", "KEGG:K00009", "GO:0005829", "GO:0008926", "GO:0019592" ] }, "pscc": { "uniref50_id": "UniRef50_P78008", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78008" ], "product": "Mannitol-1-phosphate 5-dehydrogenase" } }, "id": "GIHJHMOALN_1387", "locus": "GCA009939745_01387" }, { "type": "cds", "sequence": "contig_1", "start": 780572, "stop": 781003, "strand": "+", "frame": 2, "gene": "mtlF", "product": "Mannitol-specific phosphotransferase enzyme IIA component", "db_xrefs": [ "COG:COG4668", "COG:G", "GO:0005737", "GO:0005886", "GO:0009401", "GO:0016301", "GO:0090563", "RefSeq:WP_010875010.1", "SO:0001217", "UniParc:UPI000013295B", "UniRef:UniRef100_P75145", "UniRef:UniRef50_P75145", "UniRef:UniRef90_P75145" ], "nt": "GTGAAATTACTAAAAAATAACATTTACATTAACGTTTATTTAAAAAACAAACAAGAAATTTTTGAATTTGTTTTTAAAAAGTTTAAAGAAGATGGTGCTGTACTAGATAGTTTTTTACCAGCAATTGTGGAACGCGATAAAGCAGCTAGTGTAGCAATTGGTAATTACCTCTTTTTACCCCACCCAGTTTATGACGAAATAGCAAACATTCAAAAAGAAAAAATGGTGTTTATTGGTTTAAAAGATGTAATTAACATTGATGGTCAACCAATTAAATTTATCTGTGGTTTAGCCTTAAAAGGTGAGCACCAAATGGATGCGTTGCAATCTTTAGCAATAGCTTTTTCAGACCCTGAAGAAGTGGAAAAGTTAGTTAAAGATAAGGATTTAACACAAGATAAGGTACTTGAATTTTTAGCAAAACACAACTAA", "aa": "MKLLKNNIYINVYLKNKQEIFEFVFKKFKEDGAVLDSFLPAIVERDKAASVAIGNYLFLPHPVYDEIANIQKEKMVFIGLKDVINIDGQPIKFICGLALKGEHQMDALQSLAIAFSDPEEVEKLVKDKDLTQDKVLEFLAKHN", "aa_hexdigest": "c06514f8b7e06b0a390b0475ecc1436f", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013295B", "ncbi_nrp_id": "WP_010875010.1", "uniref100_id": "UniRef100_P75145", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013295B", "RefSeq:WP_010875010.1", "UniRef:UniRef100_P75145" ] }, "ips": { "uniref100_id": "UniRef100_P75145", "uniref90_id": "UniRef90_P75145" }, "psc": { "uniref90_id": "UniRef90_P75145", "gene": "mtlF", "product": "Mannitol-specific phosphotransferase enzyme IIA component", "uniref50_id": "UniRef50_P75145", "cog_id": "COG4668", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0005886", "GO:0016301", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75145", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75145" ], "product": "Mannitol-specific phosphotransferase enzyme IIA component" }, "genes": [ "mtlF" ], "id": "GIHJHMOALN_1388", "locus": "GCA009939745_01388" }, { "type": "cds", "sequence": "contig_1", "start": 781511, "stop": 781765, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "nt": "ATGTTAATGCGTAAAAAGAAACTTTTGTCAAGGATTTCCTTCGGTTCTTTGTTTTTGTTATGTGGAACAATATTAAGTGCTTGCACATGGATCCAAGCCAATCTTAGGAATCTTCTTAAAGAAACAACTGGCAAAGATTTTGATTTGTCTAAAGCAATTAAAATTCCGGAAGGTAGGCAAAACCTCATAAATTCTTTAAAGAAATCTTATGAAGTTAATCCAAAAGATACAACAAAGCTATTGTTAGACGCATGA", "aa": "MLMRKKKLLSRISFGSLFLLCGTILSACTWIQANLRNLLKETTGKDFDLSKAIKIPEGRQNLINSLKKSYEVNPKDTTKLLLDA", "aa_hexdigest": "1b5af5852a8f105a0151a9168d3fd15f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75316", "query_cov": 0.9761904761904762, "subject_cov": 0.8118811881188119, "identity": 0.8290000000000001, "score": 133.0, "evalue": 5.08e-39, "valid": false, "product": "Uncharacterized lipoprotein MPN_467", "uniref50_id": "UniRef50_P75316", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75316" ], "product": "Uncharacterized lipoprotein MPN_467" }, "genes": [], "id": "GIHJHMOALN_1389", "locus": "GCA009939745_01389" }, { "type": "cds", "sequence": "contig_1", "start": 782042, "stop": 782656, "strand": "+", "frame": 2, "gene": null, "product": "UPF0134 protein MPN_655", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6483", "UniRef:UniRef100_A0A0H3DML3", "UniRef:UniRef50_P75136", "UniRef:UniRef90_P75136" ], "nt": "ATGAGCAACAATAATAAATATTTCACTATAACTAAAAAACAGTACAAAAAAATGCGCCGCAATAAAATTGAACTTCTTTTTAATGTGAAAGTCCTAAAAAAGAAAAACGGTCGGCAAAAGTTCAAAATACTGCATGAAGTTGAGAATAAGCCAAAAATACCAATTAAAATAATTGAAGATCAACCGGAGTCGCCAAAGCCACCTAAAACTCCTAAACCCCCTAAACCGCCTAAAGGTCCTGACAATCCTGAGGAGCCGGATTCTCCAGAAGAACCCAAAGAAACAGATCAACCAGGTGGTCCCGATAATCCTAATGCAGGAAATAAAAAGATGCCAACACCTGAAGAATACGTAACTCGTAAAGAATTCAACGAATTCAAAGATTCAAACAATCAACGACTTACAAAGATTGAAAATAAGGTTGATAAGTTAGAAGTCAAAGTCGACAAATTAGCTGAAATTGTTCAAACTCAGGGCGAAGAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGGGTGAAACCCTACAGCTAATTTTGAAAACACTGCAAAAGATGAACGATCGTCTGGACAGAATGGAAACTCGCTTAGACAAGATCGATTCGCCCAAATAG", "aa": "MSNNNKYFTITKKQYKKMRRNKIELLFNVKVLKKKNGRQKFKILHEVENKPKIPIKIIEDQPESPKPPKTPKPPKPPKGPDNPEEPDSPEEPKETDQPGGPDNPNAGNKKMPTPEEYVTRKEFNEFKDSNNQRLTKIENKVDKLEVKVDKLAEIVQTQGEEIKELKVEQKAQGETLQLILKTLQKMNDRLDRMETRLDKIDSPK", "aa_hexdigest": "fff37ba2429770b9ee5da4c9d1bf9a3d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6483", "uniref100_id": "UniRef100_A0A0H3DML3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6483", "UniRef:UniRef100_A0A0H3DML3" ] }, "ips": { "uniref100_id": "UniRef100_A0A0H3DML3", "uniref90_id": "UniRef90_P75136" }, "psc": { "uniref90_id": "UniRef90_P75136", "product": "UPF0134 protein MPN_655", "uniref50_id": "UniRef50_P75136" }, "pscc": { "uniref50_id": "UniRef50_P75136", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75136" ], "product": "UPF0134 protein MPN_655" }, "genes": [], "id": "GIHJHMOALN_1390", "locus": "GCA009939745_01390" }, { "type": "cds", "sequence": "contig_1", "start": 782744, "stop": 783022, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5N8", "UniRef:UniRef90_A0AB38W5N8" ], "nt": "TTGTTGGCACTTTTTAATTGCTATTTCAATTTATTTAGTGGAGGAGCAAAATGCAATTTTTTTGATTTTCTAGAATCCTCTAGAAAGGAGGTGTTAATTATGAAATATAAAAAGATAGGTGATATTAAATCTAAAGGATTTTTCGAATCTAAAGAAGAATATAAGGAACTTTTAAAAAAATGTGAACTAATTTCTTTTGAAGCTTTTTCTGAAAAATTACCTAATGGTGATTATGAAGTGACTAAGAAACTTAAGCTTGTAGAAAAAGGTCCAAATTGA", "aa": "MLALFNCYFNLFSGGAKCNFFDFLESSRKEVLIMKYKKIGDIKSKGFFESKEEYKELLKKCELISFEAFSEKLPNGDYEVTKKLKLVEKGPN", "aa_hexdigest": "96ed48760334b5378b730723f4d0931c", "start_type": "TTG", "rbs_motif": "AAAAA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 782771, "stop": 783091, "observations": { "mutation": [ 783020 ], "stop": [ 783020 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB38W5N8", "query_cov": 0.9021739130434783, "subject_cov": 0.7830188679245284, "identity": 0.988, "score": 160.0, "evalue": 2.48e-49, "gene_start": 10, "gene_end": 92, "reference_start": 1, "reference_end": 83, "reference_sequence": "NLFSGWAKCNFFDFLESSRKEVLIMKYKKIGDIKSKGFFESKEEYKELLKKCELISFEAFSEKLPNGDYEVTKKLKLVEKGPNWKRKTYKQSLENEKILKWLIEHD" }, "paralog": false, "identity": 0.9811320754716981, "score": 210.0, "evalue": 1.74e-71, "description": "Internal stop codon at 783020. Nonsense mutation around 783020.", "psc": { "uniref90_id": "UniRef90_A0AB38W5N8", "uniref50_id": "UniRef50_A0AB38W5N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB38W5N8", "UniRef:UniRef50_A0AB38W5N8" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5N8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10712.412399999992, "isoelectric_point": 8.717108726501465 }, "id": "GIHJHMOALN_1391", "locus": "GCA009939745_01391" }, { "type": "cds", "sequence": "contig_1", "start": 783131, "stop": 783451, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCGATACCCCAGGAGTCTTCTTTAAACGAATTGATGAAATGGCTGTTGGTTACAAGTTAGTTTTAACTAACGTTATTAAAAGGGAAGTAGTCCCTTTGGAAGATGTAGGTGCGTTTGCTTTTTGTTATTTAAGCAAACATTATCCGCAATTGTTACCATATGAAGGTACAGACTTCACTGAGTTTTTACATAAATTTGCTATCAGTCGTAAGTTGTTGCAAAAATCGAATCAGCTCAACATTAATTTAGCGTGCGAACTGTTTGTTAGTGAACTGATAAACGGGAAGTACGGTAAACTGAGTTTTGAGTTAGATTAG", "aa": "MSDTPGVFFKRIDEMAVGYKLVLTNVIKREVVPLEDVGAFAFCYLSKHYPQLLPYEGTDFTEFLHKFAISRKLLQKSNQLNINLACELFVSELINGKYGKLSFELD", "aa_hexdigest": "9ca259566b1ccafaf0ec49739a05e34a", "start_type": "TTG", "rbs_motif": "AATAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12155.996799999988, "isoelectric_point": 6.096573066711424 }, "id": "GIHJHMOALN_1392", "locus": "GCA009939745_01392" }, { "type": "cds", "sequence": "contig_1", "start": 783476, "stop": 783946, "strand": "-", "frame": 3, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254", "SO:0001217", "UniRef:UniRef50_P75135", "UniRef:UniRef90_P75135" ], "nt": "ATGGATACATTTACCTCCGCAGTCAAGATTAACTGGTTTCCGGGTCACATGAAGAAGACCCATGACCAACTCAAAAAACTAGCTAGTAGTTTAGATGGTGTAATTGAGGTAGTTGATGCGCGTGCACCAACGTTAACCCAAAACCCCGAAATTACGGCTTACTTTACCAATAAACCGAAACTCACTTTAGCTTTAAAGGCTGATTTAGCACAAACAGTGGCTAACTCCAATATTTTATGGGGAACCTTAAAGCAAGGTTTGCAACTTAAACGCTTAGTTATTAAGAAACTGCAAACTTTGTTTCAAGCAAAAAAGAATCAGTTAAAAGCCAAAGGTTTATTAGTGCACCAGTTTCGGTTAGCGGTTATTGGAATGCCCAACGTTGGTAAATCCAGTTTAATTAACTTGTTACTAAATAAAAATCACTTACAGGTAGCTAATCGGGCTGGGGTTACCAAGTCAATGAGTTGA", "aa": "MDTFTSAVKINWFPGHMKKTHDQLKKLASSLDGVIEVVDARAPTLTQNPEITAYFTNKPKLTLALKADLAQTVANSNILWGTLKQGLQLKRLVIKKLQTLFQAKKNQLKAKGLLVHQFRLAVIGMPNVGKSSLINLLLNKNHLQVANRAGVTKSMS", "aa_hexdigest": "26aecbf46c5a5e62f936b20eae5dd902", "start_type": "ATG", "rbs_motif": null, "genes": [ "ylqF" ], "truncated": "5-prime", "pseudogene": { "start": 783173, "stop": 783946, "observations": { "mutation": [ 783478 ], "stop": [ 783478 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75135", "query_cov": 1.0, "subject_cov": 0.5756457564575646, "identity": 1.0, "score": 300.0, "evalue": 4.05e-101, "gene_start": 1, "gene_end": 156, "reference_start": 1, "reference_end": 156, "reference_sequence": "MDTFTSAVKINWFPGHMKKTHDQLKKLASSLDGVIEVVDARAPTLTQNPEITAYFTNKPKLTLALKADLAQTVANSNILWGTLKQGLQLKRLVIKKLQTLFQAKKNQLKAKGLLVHQFRLAVIGMPNVGKSSLINLLLNKNHLQVANRAGVTKSMSWNQISSEFYLSDTPGVFFKRIDEMAVGYKLVLTNVIKREVVPLEDVGAFAFCYLSKHYPQLLPYEGTDFTEFLHKFAISRKLLQKSNQLNINLACELFVSELINGKYGKLSFELD" }, "paralog": false, "identity": 0.9961089494163424, "score": 502.0, "evalue": 2.43e-183, "description": "Internal stop codon at 783478. Nonsense mutation around 783478.", "psc": { "uniref90_id": "UniRef90_P75135", "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_P75135", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75135", "UniRef:UniRef50_P75135", "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75135", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75135" ], "product": "Probable ribosome biogenesis GTPase A" } }, "id": "GIHJHMOALN_1393", "locus": "GCA009939745_01393" }, { "type": "cds", "sequence": "contig_1", "start": 783915, "stop": 784226, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "GTGTTATTTACGGGTACAGTAGTGAGTCACCTTTTCCCAAGGTATAACTTTGCTGAAATTAAGGTCTTTACTGACAAGATTGAAGAAGTGAGGTTGGCTCTGCTCAACGATAAAGCGACTCACAGTCTGTCCATTCAAGAAACGATGGGTGGATACTCATTAGCCAAAAAACGGATGATAGTTTCAGTAACTATGTATGTGGAAATTCCTAACCTCATCCGCATCATCCGGAAAATTGACAAAGATTGTTTAGTATCAATTACAAGGATCCGAGGGATCGATGGATACATTTACCTCCGCAGTCAAGATTAA", "aa": "MLFTGTVVSHLFPRYNFAEIKVFTDKIEEVRLALLNDKATHSLSIQETMGGYSLAKKRMIVSVTMYVEIPNLIRIIRKIDKDCLVSITRIRGIDGYIYLRSQD", "aa_hexdigest": "33dee91c770c25c59834e4626163ef0f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 52, "aa_cov": 0.5048543689320388, "hmm_cov": 1.0, "evalue": 2.5040859025912754e-11, "score": 38.782386779785156, "start": 44, "stop": 95 } ], "seq_stats": { "molecular_weight": 11882.873999999985, "isoelectric_point": 9.371398353576659 }, "id": "GIHJHMOALN_1394", "locus": "GCA009939745_01394" }, { "type": "cds", "sequence": "contig_1", "start": 784275, "stop": 784460, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGATCTTGTGCTGGGGGTGCCGATTTCCTAACGCAATACTTTGCGAGAAAGAAAAACCGCTCCGTGGGGCCAATTCTCTTTTATGTCAATACCTTTATTTTGATCATTGCGATCTTAATGGGTTCGTTTGTCGCTGGCAGTATTGTGCTCCAAGATATTCCCGATTACAAAAAAAGTGCTTGA", "aa": "MGSCAGGADFLTQYFARKKNRSVGPILFYVNTFILIIAILMGSFVAGSIVLQDIPDYKKSA", "aa_hexdigest": "0644a8e2ac08d27f410a46c58827d58e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6618.7403, "isoelectric_point": 9.35966510772705 }, "id": "GIHJHMOALN_1395", "locus": "GCA009939745_01395" }, { "type": "cds", "sequence": "contig_1", "start": 784557, "stop": 785024, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAGTGCCCTTTATGAGGCTAAAAAACCTTTAAAGTACACAATTGTCTACATCCTGGCTTTAGTGAACGCCTTCTTTTTGCTGGTGTTTATCCAACAAACGGGATTGTACAGCTTTGGGATTAGTTCGCTAACCCAAGGTTTTGCGCGTTTACTGTTTGTCTTGTTGAAGAACCTCGAAGATGGACAACGCAACCTAGTGTTCAACATCTTTTACTGGCTGTTTTATGTAATAGTCAACATTCCCCTGATTATTTTTTCTTACAAAAAAATTGGGAAACGTTTCACCATTCTGTCAACCCATTACGTGGTCGCTTCCAACGTCTTTGGTTTTATCTTTAGTATTATTCCGGGGGCTAATCAATTGCCATCGATGTTGTCCGCAGTGCACCACACTGAATTTTGGGAAGCTGCAAAAAAAGCTGAAGGTGTTGATCAAAGCGCGTTATTTGTACCGTTCTTATGA", "aa": "MFSALYEAKKPLKYTIVYILALVNAFFLLVFIQQTGLYSFGISSLTQGFARLLFVLLKNLEDGQRNLVFNIFYWLFYVIVNIPLIIFSYKKIGKRFTILSTHYVVASNVFGFIFSIIPGANQLPSMLSAVHHTEFWEAAKKAEGVDQSALFVPFL", "aa_hexdigest": "91321d7a6c83d5dd4d0d5bdd0788acb5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17692.718899999985, "isoelectric_point": 9.487441444396975 }, "id": "GIHJHMOALN_1396", "locus": "GCA009939745_01396" }, { "type": "cds", "sequence": "contig_1", "start": 785136, "stop": 785495, "strand": "-", "frame": 2, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "COG:COG0335", "COG:J", "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_010875015.1", "SO:0001217", "UniParc:UPI0000133CB4", "UniRef:UniRef100_P75133", "UniRef:UniRef50_Q8EWU9", "UniRef:UniRef90_P75133" ], "nt": "ATGAAAAAAATAAATAAACAAGCTTTAATAGATTTAGTAGAACAAAAGCAATTAAAGGCTTATGTGCCTGAGTTTAGCGCTGGTGACGAAGTGAATGTTGCCATCAAACTTAAAGAAAAGGAAAAGGTCAGAATCCAAAACTTTACGGGAACTGTCCTACGCCGTCGTGGTAAAGGTATTAGTGAAACCTTCATTGTGCGTAAAACCACTGATGGTATTCCGATTGAAAAAAACTTCCAAATTCATAACCCTAATATTTCAATTGAATTGAAAAGACGGGGTAAAGTAAGACGCGCTTACATTTCATATATGCGTGAGCGCTCTGGTAAAGCTGCCAAAATTAAAGAGCGAAAACAATAG", "aa": "MKKINKQALIDLVEQKQLKAYVPEFSAGDEVNVAIKLKEKEKVRIQNFTGTVLRRRGKGISETFIVRKTTDGIPIEKNFQIHNPNISIELKRRGKVRRAYISYMRERSGKAAKIKERKQ", "aa_hexdigest": "5d27fe9483784a60add336e9224bdb23", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133CB4", "ncbi_nrp_id": "WP_010875015.1", "uniref100_id": "UniRef100_P75133", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133CB4", "RefSeq:WP_010875015.1", "UniRef:UniRef100_P75133" ] }, "ips": { "uniref100_id": "UniRef100_P75133", "uniref90_id": "UniRef90_P75133" }, "psc": { "uniref90_id": "UniRef90_P75133", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_Q8EWU9", "cog_id": "COG0335", "cog_category": "J", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EWU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EWU9" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "GIHJHMOALN_1397", "locus": "GCA009939745_01397" }, { "type": "cds", "sequence": "contig_1", "start": 785479, "stop": 786021, "strand": "-", "frame": 1, "gene": null, "product": "tRNA (guanine-N(1)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "nt": "ATGGCTTACGGAGGAGGTGGCGGGATGATTCTAAAAGCTGAACCAATTCTCAAAGCTTTAAAGGCTTGTAGAACTCCACAATCGAAAGTAGTTTTATTATCACCTGAGGGACAACAGTTTTCCCAACCCATGGCGCAAGCTTTAACCCAAACGGAACACCTCATTTTGATCTGTGGTCACTATGAGGGCTTTGATTACCGCTTGTATAAACATGTCGATCAAATTATTTCGTTAGGTGACTTTGTGTTGAGTGGTGGGGAGTTAGTGGCTTTAAGTGTGATTGATGCCACTGTTCGTTTAATTAAGGGTGTCATTAATGATCAGAGCTTAATCCACGAATCATTTAACAACTACTTACTGGATTTCCCCGCTTACACTAGGCCATATGATCTAGACGGGGATAAGGTACCTGAAATATTATTATCAGGTGACCATAAAAAGATAGAGGCTTATCGCAAAGAACAACAACTGCTCAGAACGGCTCAATACCGTCCTGATTTGTATAAGCAATATTTAGCAAAGAAAGATGAAAAAAATAAATAA", "aa": "MAYGGGGGMILKAEPILKALKACRTPQSKVVLLSPEGQQFSQPMAQALTQTEHLILICGHYEGFDYRLYKHVDQIISLGDFVLSGGELVALSVIDATVRLIKGVINDQSLIHESFNNYLLDFPAYTRPYDLDGDKVPEILLSGDHKKIEAYRKEQQLLRTAQYRPDLYKQYLAKKDEKNK", "aa_hexdigest": "bbba8a27843b964f59f5fd9a127acf63", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A534NV99", "query_cov": 0.9444444444444444, "subject_cov": 0.821256038647343, "identity": 0.535, "score": 171.0, "evalue": 6.91e-51, "valid": false, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_B1GZ20", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_B1GZ20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [], "id": "GIHJHMOALN_1398", "locus": "GCA009939745_01398" }, { "type": "cds", "sequence": "contig_1", "start": 786288, "stop": 786437, "strand": "-", "frame": 2, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627", "SO:0001217", "UniRef:UniRef50_P47684", "UniRef:UniRef90_P75131" ], "nt": "ATGGGTCGTGTTCACTACCCAACCTACAGAATAGTAGCTGTTGATTCCAGAGTTAAACGTGATGGTAAGTACATTGCGTTAATTGGCCATCTCAATCCTGCATTAAAAGAAAATAAGTGCAAGATTGATGAAGCTGTGGCCTTGGAATGA", "aa": "MGRVHYPTYRIVAVDSRVKRDGKYIALIGHLNPALKENKCKIDEAVALE", "aa_hexdigest": "637d190f7323f0450db83dfbabd2a9be", "start_type": "ATG", "rbs_motif": "AAAAA", "genes": [ "rpsP" ], "truncated": "5-prime", "pseudogene": { "start": 786177, "stop": 786461, "observations": { "mutation": [ 786290 ], "stop": [ 786290 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75131", "query_cov": 1.0, "subject_cov": 0.5212765957446809, "identity": 1.0, "score": 102.0, "evalue": 1.49e-27, "gene_start": 1, "gene_end": 49, "reference_start": 9, "reference_end": 57, "reference_sequence": "MVKIRLMRMGRVHYPTYRIVAVDSRVKRDGKYIALIGHLNPALKENKCKIDEAVALEWLNKGAKPTDTVRSLFSQTGLWKKFVESKKKPVAKSK" }, "paralog": false, "identity": 0.9787234042553191, "score": 184.0, "evalue": 2.84e-62, "description": "Internal stop codon at 786290. Nonsense mutation around 786290.", "psc": { "uniref90_id": "UniRef90_P75131", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_P47684", "cog_id": "COG0228", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75131", "UniRef:UniRef50_P47684", "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47684", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47684" ], "product": "Small ribosomal subunit protein bS16" } }, "id": "GIHJHMOALN_1399", "locus": "GCA009939745_01399" }, { "type": "cds", "sequence": "contig_1", "start": 786525, "stop": 786749, "strand": "-", "frame": 2, "gene": "norM", "product": "putative MG447-like protein", "db_xrefs": [ "COG:COG0534", "COG:V", "GO:0005886", "GO:0015297", "GO:0042910", "RefSeq:WP_243679576.1", "SO:0001217", "UniParc:UPI000AC194D4", "UniRef:UniRef100_P75130", "UniRef:UniRef50_P47685", "UniRef:UniRef90_P75130" ], "nt": "ATGCAAGGGGTTATTGTTTTTGTACCCTTGTTGTTTATCTTTCAAGCAATTACCTTACAAACAAAAAACATTGAAATCTTTATCTGGTTATTAACGGCTAACGCTGCTCTGGCTGGATTGATTAACGTTTTGATTGGCCAAGTACACATCCACTTCTTTATGGATAAATACTTCGCTCAAAAACATAAGAGTCGGATTGTCCAGTTCATTGAGCGCTATTCTTAG", "aa": "MQGVIVFVPLLFIFQAITLQTKNIEIFIWLLTANAALAGLINVLIGQVHIHFFMDKYFAQKHKSRIVQFIERYS", "aa_hexdigest": "3b5ab56965889da8b0b63069a76e0208", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000AC194D4", "ncbi_nrp_id": "WP_243679576.1", "uniref100_id": "UniRef100_P75130", "db_xrefs": [ "SO:0001217", "UniParc:UPI000AC194D4", "RefSeq:WP_243679576.1", "UniRef:UniRef100_P75130" ] }, "ips": { "uniref100_id": "UniRef100_P75130", "uniref90_id": "UniRef90_P75130" }, "psc": { "uniref90_id": "UniRef90_P75130", "gene": "NorM", "product": "Uncharacterized protein MG447 homolog", "uniref50_id": "UniRef50_P47685", "cog_id": "COG0534", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015297", "GO:0042910" ] }, "pscc": { "uniref50_id": "UniRef50_P47685", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47685" ], "product": "Uncharacterized protein MG447" }, "genes": [ "norM" ], "id": "GIHJHMOALN_1400", "locus": "GCA009939745_01400" }, { "type": "cds", "sequence": "contig_1", "start": 786762, "stop": 788174, "strand": "-", "frame": 2, "gene": "norM", "product": "putative MG447-like protein", "db_xrefs": [ "COG:COG0534", "COG:V", "GO:0005886", "GO:0015297", "GO:0042910", "SO:0001217", "UniRef:UniRef50_P47685", "UniRef:UniRef90_P75130" ], "nt": "GTGAATAGCCAACAAGATCGTCTAGCGAAGCAAGTCCTCATCCAAAAATCCTTTGAATCGAAAAGACTGTTTCTAACGATCTTGCGCTTTGCTATTCCGACTTTTTTCTTTGCGCTGTTTTCCGCTGCTTATGTCTTTGTGGACCAAATTATGGTGATTAAGTTTGTCCCGCATGGACCACTTAATCCCGATTCAATCTTTACTGACCAGGCCTTAATTGAGGAATTTAAAGCATCCGCGTTTTATAAGGGTGGGGACATTCCCAACCACACGGAACTAACAGCTAGTCAATTAGTCAAAACAGTCTTAAATATTAGCCAGCCAATTGTAGTCATTCTCAACGCCATTACCATCTTTGTGCCGTTGGGTACGGGGGTGATCTTTTCCAAAACCATTGGTAAGGGTGATGAGAAAAAGATTAAGGATGCTTGGAATACCGGGTTAGTTTCCACTACCTTATTTGCCCTAGTGACCCAAATTATTGTGCTAGCGATTGCCAAAGAGTGGTTGCAGTTTAACTTGGATAAAGTCGATGAACAACACCACGTGCAAGTAGCGGATCAGTTTCAACACTTCTTTAACGAAAAGGCGGTTGCGATTGGTTCAGAGTACGTTTACATTTTGATTGGCTTTAACATCATACCGATGTTGAGCCGCTTGTTTTTTTACTTAGGCCAATCAGAGGGCCGGCAACTGTTTATTGCAATTGTGCCTCCTTTATCGAACTTACTGAACGTCCTGTTTGTTTTCTTGTTAGTGCGTTTTTCCACTTTAGGGGTTGTGGGCTCCGCGGTGGCTGCGATTTTGGTTTACTTCATTACCTTTATGGCTTATGTGGTGTACCTTATTAGTTTAAATAAACGTGGTTTAACCTACCTTTCGTTGAGGGACTTTTCCTTTAAACGGGTTAGTTTTAACCTCTTTTTGGTCATTTCAATGGTGGGGTTAGCCTCCTTTTTCCGTAATGGTTCGCTCTCAATTCTCAACACCTTTTACGAAAGCTTTTTGGTGAATTTGACCAAAACGCTTACGGATCAAAGTGATACCTTTTACTTGGTGTTACTAACGGGACCCATTGCAATTGCTAACCTAACTTCAGCCGCCATCTTTGGTGTTCTCCAAGGGGTGCGGACAGTGGTATCCTACAAGTTTGGTCAGGGCCAGTTGGCTGACATTAAAAGGATTAATGTTTATACGTTGTTAGTGTGTTTGGTATTTGCTGCTTTGCTGTATTTAATTCTGGCAGTTGGATTGGGCAAAGAAATCCTAGTGCACCTGTTTGATACAAGTGCAGCTACCTTAATGCTCGCTAATCAGTTTTCCTTAATTGTGCAAGCGCAAGTGTTCTTTGTGGCCATTGGCGCTACCAGTCAACAGTACTTCCAAAATACCAACCGGGTGTTGTATTCGTGA", "aa": "MNSQQDRLAKQVLIQKSFESKRLFLTILRFAIPTFFFALFSAAYVFVDQIMVIKFVPHGPLNPDSIFTDQALIEEFKASAFYKGGDIPNHTELTASQLVKTVLNISQPIVVILNAITIFVPLGTGVIFSKTIGKGDEKKIKDAWNTGLVSTTLFALVTQIIVLAIAKEWLQFNLDKVDEQHHVQVADQFQHFFNEKAVAIGSEYVYILIGFNIIPMLSRLFFYLGQSEGRQLFIAIVPPLSNLLNVLFVFLLVRFSTLGVVGSAVAAILVYFITFMAYVVYLISLNKRGLTYLSLRDFSFKRVSFNLFLVISMVGLASFFRNGSLSILNTFYESFLVNLTKTLTDQSDTFYLVLLTGPIAIANLTSAAIFGVLQGVRTVVSYKFGQGQLADIKRINVYTLLVCLVFAALLYLILAVGLGKEILVHLFDTSAATLMLANQFSLIVQAQVFFVAIGATSQQYFQNTNRVLYS", "aa_hexdigest": "839e66ae9cb97e64f5585f52f3ebfbf1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75130", "query_cov": 1.0, "subject_cov": 0.8561020036429873, "identity": 0.996, "score": 823.0, "evalue": 5.06e-298, "valid": true, "gene": "NorM", "product": "Uncharacterized protein MG447 homolog", "uniref50_id": "UniRef50_P47685", "cog_id": "COG0534", "cog_category": "V", "go_ids": [ "GO:0005886", "GO:0015297", "GO:0042910" ] }, "pscc": { "uniref50_id": "UniRef50_P47685", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47685" ], "product": "Uncharacterized protein MG447" }, "genes": [ "norM" ], "id": "GIHJHMOALN_1401", "locus": "GCA009939745_01401" }, { "type": "cds", "sequence": "contig_1", "start": 788175, "stop": 788363, "strand": "-", "frame": 2, "gene": null, "product": "peptide-methionine (R)-S-oxide reductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024947FD0" ], "nt": "ATGATCCGCACCGAAGTACGCGCCAAAAACTCCAACAGCCACCTAGGGCACGTCTTTCGTGATGGTCCCGAAGAGCATGGTGGTTTGCGTTACTGCATTAACTCCGCTGCTCTAAAGTTCATTCCGTTTGCGGAAATGGAATCGGCGGGGTATGGTGAATACCTCAAATTATTTAAGGAATCTAACTAA", "aa": "MIRTEVRAKNSNSHLGHVFRDGPEEHGGLRYCINSAALKFIPFAEMESAGYGEYLKLFKESN", "aa_hexdigest": "280923d08d9886123ebc2193d66ab6d1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024947FD0", "query_cov": 0.9193548387096774, "subject_cov": 0.8769230769230769, "identity": 0.7190000000000001, "score": 93.2, "evalue": 7.58e-24, "valid": false, "product": "peptide-methionine (R)-S-oxide reductase", "uniref50_id": "UniRef50_UPI0024947FD0" }, "pscc": { "uniref50_id": "UniRef50_UPI0024947FD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024947FD0" ], "product": "peptide-methionine (R)-S-oxide reductase" }, "genes": [], "id": "GIHJHMOALN_1402", "locus": "GCA009939745_01402" }, { "type": "cds", "sequence": "contig_1", "start": 788427, "stop": 788630, "strand": "-", "frame": 2, "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "db_xrefs": [ "COG:COG0229", "COG:O", "EC:1.8.4.12", "GO:0005737", "GO:0006979", "GO:0030091", "GO:0033743", "SO:0001217", "UniRef:UniRef50_Q9CJ17", "UniRef:UniRef90_P75129" ], "nt": "ATGAGTAAGTACCAAAAAAAGAGTGACGGGGAACTAAAGCGGACCTTAACTAAACTACAGTACGACGTTACCCAAAACGCCCACACCGAACCGCCCTATACTAATGAATATAACCGCCATTACGAAAAGGGTATTTACGTTGACATTACGTCCGGTGAACCGTTGTTTATCTCAACTGATAAGTTCAAATCCGGTTGTGGTTGA", "aa": "MSKYQKKSDGELKRTLTKLQYDVTQNAHTEPPYTNEYNRHYEKGIYVDITSGEPLFISTDKFKSGCG", "aa_hexdigest": "e6168f0acf4796d952af691d466aa850", "start_type": "ATG", "rbs_motif": null, "genes": [ "msrB" ], "truncated": "5-prime", "pseudogene": { "start": 788175, "stop": 788630, "observations": { "mutation": [ 788429 ], "stop": [ 788429 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75129", "query_cov": 1.0, "subject_cov": 0.44370860927152317, "identity": 1.0, "score": 143.0, "evalue": 1.77e-42, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MSKYQKKSDGELKRTLTKLQYDVTQNAHTEPPYTNEYNRHYEKGIYVDITSGEPLFISTDKFKSGCGWPAFTKPISQDLIANYRDESHGMIRTEVRAKNSNSHLGHVFRDGPEEHGGLRYCINSAALKFIPFAEMESAGYGEYLKLFKESN" }, "paralog": false, "identity": 0.9933774834437086, "score": 311.0, "evalue": 2.52e-111, "description": "Internal stop codon at 788429. Nonsense mutation around 788429.", "psc": { "uniref90_id": "UniRef90_P75129", "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "ec_ids": [ "1.8.4.12" ], "uniref50_id": "UniRef50_Q9CJ17", "cog_id": "COG0229", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0033743", "GO:0030091", "GO:0006979" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75129", "EC:1.8.4.12", "UniRef:UniRef50_Q9CJ17", "COG:COG0229", "COG:O", "GO:0005737", "GO:0033743", "GO:0030091", "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_Q9CJ17", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9CJ17" ], "product": "Peptide methionine sulfoxide reductase MsrB" } }, "id": "GIHJHMOALN_1403", "locus": "GCA009939745_01403" }, { "type": "cds", "sequence": "contig_1", "start": 788627, "stop": 789298, "strand": "-", "frame": 3, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "db_xrefs": [ "COG:COG0073", "COG:J", "GO:0000049", "SO:0001217", "UniRef:UniRef50_P47687", "UniRef:UniRef90_P75128" ], "nt": "ATGCGTTACGGTTTTTTTGATATTTCTCAGGACTTTATCACCATTTTTTGTCCCAAGAAAACACTTAAGAACTGTATGTTTGGTTTAATTGGTAGCCGCACGCAAGCTACTTTGCGCCAAGAAAAGAACCAGAACTTTAGCTTCTTTGTCAATGAAGCCAATGAGATTGCTGGGTTTAACTTCTTTGACATTAAAAAGAGCTTTCGCAGGGGATTAATTTCCCATCACTTTACCGCAGGGTTGAATTACCCTTCTTTGAAGTTGGTCAAAAAGATTAGTGAGCTGTTGAACTATGATTTAACACCCTTAGCCAAAAAGGTACCCTTTGTGGTTTGTGAGGTCATTTCGGCCATTCCGATTCCCAACACTCATTTGAAAAGGTGTAAGGTCAACACTGGTTCCAATAAGTCCTTGGATGTCGTGTGTGGGGCGGATAATGTTCGGGTTGGCCTTAAAACTGTTTTAGTCCATGTTGGTGGTGTTTTACCTGATGGCACGATTATCAAGAAGGCTAAAATTGCGGGGTATGATTCCATGGGAATGCTCTGTTCGGAAAAAGAGCTACATTTAAAACCTAAAAATCAGGGTATAATTGAAATTAAGTCGCACATCAAAATAGGCAAAAGTTTCCTTGATGTTTACTTAAATAATAGTGAAAAATTCAGTGCATGA", "aa": "MRYGFFDISQDFITIFCPKKTLKNCMFGLIGSRTQATLRQEKNQNFSFFVNEANEIAGFNFFDIKKSFRRGLISHHFTAGLNYPSLKLVKKISELLNYDLTPLAKKVPFVVCEVISAIPIPNTHLKRCKVNTGSNKSLDVVCGADNVRVGLKTVLVHVGGVLPDGTIIKKAKIAGYDSMGMLCSEKELHLKPKNQGIIEIKSHIKIGKSFLDVYLNNSEKFSA", "aa_hexdigest": "93a2d687b612a5aaf80bb0ed3450344b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75128", "query_cov": 1.0, "subject_cov": 0.9529914529914529, "identity": 0.996, "score": 447.0, "evalue": 5.38e-159, "valid": true, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "uniref50_id": "UniRef50_P47687", "cog_id": "COG0073", "cog_category": "J", "go_ids": [ "GO:0000049" ] }, "pscc": { "uniref50_id": "UniRef50_P47687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47687" ], "product": "Uncharacterized protein MG449" }, "genes": [ "ytpR" ], "id": "GIHJHMOALN_1404", "locus": "GCA009939745_01404" }, { "type": "cds", "sequence": "contig_1", "start": 789305, "stop": 790177, "strand": "-", "frame": 3, "gene": "degV", "product": "DegV domain-containing MG450-like protein", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "RefSeq:WP_014575051.1", "SO:0001217", "UniParc:UPI0001DD6489", "UniRef:UniRef100_A0AAV5N7U0", "UniRef:UniRef50_P47688", "UniRef:UniRef90_P75127" ], "nt": "GTGAGAGTCGCATTCTTAGTTGACTCTGTTAGTAACCTCAAGGAAAGCCAGGCACAACATCTGTATGTGCTACCGCTTTTCATCATTGAATCAACTCCTGAAGCTGAAACCACCTTTAGTTCGGGGTTTGACATTGATTTAAAAACCCTCACGGACAAGATGGCTAACGCTCCGAAGGGAGTGAAGTTCTCCACATCGCAAACTACTGAAGAAATAGTGCGCGCTAAGGTGAGTGAACTAGTGGAGCAGTATGACCTGATTATCGGCATTCCGATTGATAAGGAAATCTCCGCTTCGTACGCGAACTGGAAGTTAGTTGAAAAGGATTACGCTTCGAAGTTCCATGTATTGGATGCCAGAGCGGTGGAAATGATCATTGATTGGCTAATCAATGACATTAAGGGTTGGTTGAATACCAATCCTTATTCCAGGGAAGGCTTGGATCGCTTTGTGGAGCAGTACCGCAAGAAAACGGCCGCAGTGTTGTTTGTCACTGATACCAAACCACTAGTGGCAGGCGGGCGGCTTTCCAATCTTAAATCCTTTATTATCAAGAGCTTAAAGTTTCACTTACTGATTAGTTTTTTAGGTGAAAATGGTAAGTTACAGTTCTTTGATAAAGCGCGTTCGACTCATGATGCGCATAAGCTAGCAGTCAAGTTTTTAAAGAAACAGCTGTTAAAACACTCCGCTAAACTGAAACGGGGTGCCTTTCTTACCACCGTCTTTGACGAACAGACCAACACTAATTTAGTCCAAGAGTTTGATAAACTGTTGGATCATTCCATTAATATAGAACAGAGCTTACTGTCCCCGGTCATTTGTACCCACACGGGATTGAACAGCTATGTAATTGTGCTGCAAGGCGAATAG", "aa": "MRVAFLVDSVSNLKESQAQHLYVLPLFIIESTPEAETTFSSGFDIDLKTLTDKMANAPKGVKFSTSQTTEEIVRAKVSELVEQYDLIIGIPIDKEISASYANWKLVEKDYASKFHVLDARAVEMIIDWLINDIKGWLNTNPYSREGLDRFVEQYRKKTAAVLFVTDTKPLVAGGRLSNLKSFIIKSLKFHLLISFLGENGKLQFFDKARSTHDAHKLAVKFLKKQLLKHSAKLKRGAFLTTVFDEQTNTNLVQEFDKLLDHSINIEQSLLSPVICTHTGLNSYVIVLQGE", "aa_hexdigest": "dcda69a3c57f3cc450f1ec1172b476f9", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6489", "ncbi_nrp_id": "WP_014575051.1", "uniref100_id": "UniRef100_A0AAV5N7U0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6489", "RefSeq:WP_014575051.1", "UniRef:UniRef100_A0AAV5N7U0" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N7U0", "uniref90_id": "UniRef90_P75127" }, "psc": { "uniref90_id": "UniRef90_P75127", "gene": "DegV", "product": "DegV domain-containing protein MG450 homolog", "uniref50_id": "UniRef50_P47688", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P47688", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47688" ], "product": "DegV domain-containing protein MG450" }, "genes": [ "degV" ], "id": "GIHJHMOALN_1405", "locus": "GCA009939745_01405" }, { "type": "cds", "sequence": "contig_1", "start": 790177, "stop": 791361, "strand": "-", "frame": 1, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "COG:COG0050", "COG:J", "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020", "RefSeq:WP_010875022.1", "SO:0001217", "UniParc:UPI0000129DB8", "UniRef:UniRef100_P23568", "UniRef:UniRef50_P23568", "UniRef:UniRef90_P23568" ], "nt": "ATGGCAAGAGAGAAATTTGACCGATCTAAACCCCACGTTAATGTAGGTACTATTGGCCACATTGACCACGGTAAAACAACTTTAACAGCAGCTATTTGTACTGTATTAGCAAAAGAAGGTAAATCAGCTGCTACTCGTTACGACCAAATCGATAAGGCTCCGGAAGAAAAAGCACGGGGAATTACGATTAACTCCGCTCACGTGGAGTACTCCTCTGACAAGCGTCACTATGCTCACGTTGACTGTCCAGGACACGCTGACTACATTAAGAACATGATTACTGGTGCTGCACAAATGGATGGTGCCATTCTAGTAGTTTCAGCAACTGACAGTGTTATGCCCCAAACCCGTGAACACATTTTGTTGGCCCGCCAAGTGGGTGTGCCACGCATGGTAGTGTTCCTAAACAAGTGTGACATTGCAACTGATGAAGAAGTGCAAGAGTTAGTAGCAGAAGAGGTACGTGACTTATTAACTTCTTACGGCTTTGATGGCAAGAACACCCCTATTATTTATGGTTCTGCACTTAAAGCGCTTGAAGGTGATCCTAAGTGGGAAGCTAAGATCCATGATTTAATGAATGCAGTTGATGAATGGATTCCAACTCCTGAACGTGAAGTGGACAAACCCTTCTTGTTGGCAATCGAAGACACCATGACGATTACTGGCCGTGGTACCGTGGTTACCGGTCGGGTTGAACGTGGTGAATTGAAAGTAGGTCAAGAAATTGAAATCGTTGGTTTACGTCCAATCCGTAAAGCAGTTGTTACCGGAATCGAAATGTTCAAAAAGGAACTTGATTCAGCAATGGCTGGGGACAACGCTGGGGTATTACTCCGTGGTGTGGACCGTAAAGAAGTGGAACGTGGTCAAGTGTTAGCTAAACCAGGTTCGATTAAACCGCACAAGAAATTTAAAGCGGAAATCTATGCTTTAAAGAAGGAAGAAGGTGGTCGTCACACCGGTTTCTTAAACGGTTACCGTCCCCAATTCTACTTCCGTACTACAGACGTTACTGGTTCGATTTCCCTACCAGAAAACACCGAAATGGTGCTACCAGGTGACAATACCTCGATTACAGTTGAACTAATTGCACCAATTGCTTGTGAAAAAGGTAGTAAGTTCTCCATCCGTGAAGGTGGTCGAACGGTTGGTGCTGGTTCAGTCACGGAAGTGCTTGAATAG", "aa": "MAREKFDRSKPHVNVGTIGHIDHGKTTLTAAICTVLAKEGKSAATRYDQIDKAPEEKARGITINSAHVEYSSDKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSATDSVMPQTREHILLARQVGVPRMVVFLNKCDIATDEEVQELVAEEVRDLLTSYGFDGKNTPIIYGSALKALEGDPKWEAKIHDLMNAVDEWIPTPEREVDKPFLLAIEDTMTITGRGTVVTGRVERGELKVGQEIEIVGLRPIRKAVVTGIEMFKKELDSAMAGDNAGVLLRGVDRKEVERGQVLAKPGSIKPHKKFKAEIYALKKEEGGRHTGFLNGYRPQFYFRTTDVTGSISLPENTEMVLPGDNTSITVELIAPIACEKGSKFSIREGGRTVGAGSVTEVLE", "aa_hexdigest": "7ebf3457ab79c849138f493d5b5ed8d0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129DB8", "ncbi_nrp_id": "WP_010875022.1", "uniref100_id": "UniRef100_P23568", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129DB8", "RefSeq:WP_010875022.1", "UniRef:UniRef100_P23568" ] }, "ips": { "uniref100_id": "UniRef100_P23568", "uniref90_id": "UniRef90_P23568" }, "psc": { "uniref90_id": "UniRef90_P23568", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_P23568", "cog_id": "COG0050", "cog_category": "J", "go_ids": [ "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P23568", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P23568" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "GIHJHMOALN_1406", "locus": "GCA009939745_01406" }, { "type": "cds", "sequence": "contig_1", "start": 791512, "stop": 791907, "strand": "+", "frame": 1, "gene": null, "product": "putative MG452-like protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75125", "UniRef:UniRef90_P75125" ], "nt": "TTGGGGGTAATTCCCTTATTTTTTATTTGTTTTGTTTTTGTAATTGCTGACATTGTCATCAGTTTGCAAAATAAAGGCCACATTATAGAAGAGATAGATAAGTTTACTAACCAGAGCAATGTTATGTTGCTCATTTATGCATGTTGGTATGTAAGCAAACCAAAGAGCCATTATCTCAAGAACCAGCAGTTCTTTTTGAGTGCCTTTGCTTACATCATCTTTACTTTTTTAGGCTACAACGTCATTTTGGCGGCTTCACAACAAGCTTACAGTGATAAGGATGCTTATTCCTTGGCAAGTTCTGTCTTTCTCCATGTGTTGGCACCAATTGCTTTCTTGGTTGCAGGTATAGTTAAGATGAAAACGGACAAGGATGTAACCTTTAATCATTTTTGA", "aa": "MGVIPLFFICFVFVIADIVISLQNKGHIIEEIDKFTNQSNVMLLIYACWYVSKPKSHYLKNQQFFLSAFAYIIFTFLGYNVILAASQQAYSDKDAYSLASSVFLHVLAPIAFLVAGIVKMKTDKDVTFNHF", "aa_hexdigest": "45aa88c1ce0d70b2c955d8260f249865", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 791446, "stop": 792201, "observations": { "mutation": [ 791476, 791905, 792067 ], "stop": [ 791476, 791905, 792067 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75125", "query_cov": 1.0, "subject_cov": 0.5219123505976095, "identity": 0.992, "score": 255.0, "evalue": 3.3e-84, "gene_start": 1, "gene_end": 131, "reference_start": 23, "reference_end": 153, "reference_sequence": "MEQNNCKIRTWFAKLALAQKVFLGVIPLFFICFVFVIADIVISLQNKGHIIEEIDKFTNQSNVMLLIYACWYVSKPKSHYLKNQQFFLSAFAYIIFTFLGYNVILAASQQAYSDKDAYSLASSVFLHVLAPIAFLVAGIVKMKTDKDVTFNHFWKSLGYFMIYPLVYGLYLATIPYVRGHYVSDDGKSTTYVVYGEITNTKDNPIVAWPVVICFLFIYFPLSFLAVYALQCKLLNRPLKAQFKCATNKCPK" }, "paralog": false, "identity": 0.9880478087649402, "score": 497.0, "evalue": 5.98e-182, "description": "Internal stop codon at 791476, 791905, 792067. Nonsense mutation around 791476, 791905, 792067.", "psc": { "uniref90_id": "UniRef90_P75125", "product": "Uncharacterized protein MG452 homolog", "uniref50_id": "UniRef50_P75125", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75125", "UniRef:UniRef50_P75125", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75125", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75125" ], "product": "Uncharacterized protein MG452 homolog" } }, "id": "GIHJHMOALN_1407", "locus": "GCA009939745_01407" }, { "type": "cds", "sequence": "contig_1", "start": 791926, "stop": 792069, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTATCCCTTAGTGTATGGGTTGTACTTAGCGACCATTCCTTATGTAAGGGGTCATTATGTGAGTGATGATGGTAAGTCTACTACCTATGTGGTTTATGGGGAGATTACCAACACCAAGGATAACCCGATCGTCGCTTGA", "aa": "MIYPLVYGLYLATIPYVRGHYVSDDGKSTTYVVYGEITNTKDNPIVA", "aa_hexdigest": "785f9afb9451ddad465ab75d45e2a03a", "start_type": "ATG", "rbs_motif": "AAAAA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5271.944600000002, "isoelectric_point": 5.4105833053588865 }, "id": "GIHJHMOALN_1408", "locus": "GCA009939745_01408" }, { "type": "cds", "sequence": "contig_1", "start": 792189, "stop": 792725, "strand": "+", "frame": 3, "gene": null, "product": "UTP--glucose-1-phosphate uridylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0M420" ], "nt": "ATGCCCAAAATAAGAAAAGCCGTTATTCCCGCAGCCGGACTGGGTACCAGATTACTCCCAGCCACTAAGGCCATTCCCAAAGAGATGTTGCCCTTAGTCAATAAACCCACAATTCAGTACATTGTGGAAGAAGCAGTAGCGAGTGGCATTAAGGAAATCCTAGTAATTGTGTCTTCTAAAAAGGAAGCGATTATTGACCACTTTGACTATGACTTTATCTTGGAAAATGCTTTACTCCAAAAGCACAAAGATCAAGAACACCAAGAAATTAAGGATATTGCCAACTTAGCGCACATTTACTTTGTCCGCCAAAAACACCAACACGGTTTAGGAGATGCCATTTTGCACGCCAAGTCGTTTGTTGGTAACGAAGACTTTGCCGTCCTTTTAGGTGATGATGTCGTGTTTGGTGAACAACCGGCATTAGCACAATGTATTCAAGCTTATGAACAAACAGATTGTCAGGTAATAGGCGTGCAAGAAGTGCCCCATGACCAGGTCAACAAGTATGGCATAGTAACCCCTGAAGCTAATTGA", "aa": "MPKIRKAVIPAAGLGTRLLPATKAIPKEMLPLVNKPTIQYIVEEAVASGIKEILVIVSSKKEAIIDHFDYDFILENALLQKHKDQEHQEIKDIANLAHIYFVRQKHQHGLGDAILHAKSFVGNEDFAVLLGDDVVFGEQPALAQCIQAYEQTDCQVIGVQEVPHDQVNKYGIVTPEAN", "aa_hexdigest": "80fdc0b8a704ba45efd13621b614de99", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0020C167D8", "query_cov": 0.9831460674157303, "subject_cov": 0.9259259259259259, "identity": 0.589, "score": 199.0, "evalue": 2.2e-62, "valid": false, "product": "UTP--glucose-1-phosphate uridylyltransferase", "ec_ids": [ "2.7.7.9" ], "uniref50_id": "UniRef50_A0A3D0M420", "kegg_orthology_id": "K00963" }, "pscc": { "uniref50_id": "UniRef50_A0A3D0M420", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D0M420" ], "product": "UTP--glucose-1-phosphate uridylyltransferase" }, "genes": [], "id": "GIHJHMOALN_1409", "locus": "GCA009939745_01409" }, { "type": "cds", "sequence": "contig_1", "start": 792756, "stop": 793064, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGAAAAACCAGCTGTCAACGAAGCCAAGAGCAACCTAGCCATTTTGAGCCGTTACATCCTTAAACCCAGTATTTTTACAGCTCTTAAACAAGTACCGTTTGGTGTTGGAGGTGAGTTACAGTTGACTGATGGGTTGAACTATTGTTTACAGCAAGGGGAACCCTTCTTTGCCAAGCACTTTGGTGGTACCCGGTTTGATGTCGGTACTAAAAATGGTTTTATTAAAGCGAACCTGTACACGGCTTTAAAAACCGATGCCATTACTAAGGATGAAGTCTTGGCCATCCTCAAGGAATTTGTTTAA", "aa": "MVEKPAVNEAKSNLAILSRYILKPSIFTALKQVPFGVGGELQLTDGLNYCLQQGEPFFAKHFGGTRFDVGTKNGFIKANLYTALKTDAITKDEVLAILKEFV", "aa_hexdigest": "8e0e761a25a077d24bf11a4bec7e16df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11209.897899999985, "isoelectric_point": 8.733677101135253 }, "id": "GIHJHMOALN_1410", "locus": "GCA009939745_01410" }, { "type": "cds", "sequence": "contig_1", "start": 793074, "stop": 793496, "strand": "-", "frame": 2, "gene": "osmC", "product": "Organic hydroperoxide resistance protein-like", "db_xrefs": [ "COG:COG1764", "COG:V", "EC:1.11.1.28", "GO:0006979", "KEGG:K04063", "RefSeq:WP_010875025.1", "SO:0001217", "UniParc:UPI0000139829", "UniRef:UniRef100_P75123", "UniRef:UniRef50_P47692", "UniRef:UniRef90_P75123" ], "nt": "ATGGCAGTAATTTACAAAACAACAGCACACGCTAGCGCCGGCAGGGAAGGCGTTGTCCAAACCGTTGATGGTTTTACCGTTAGTTTAGCATTTCCTAAACCTGGTGCTACTCACCAGGACAAAAACAACCCCGAACAGCTGTTTGCTTCAGCTTATGCTGGTTGTTTTTCGCAAGCTGTGCGTGTGGTTTTACAACAGCACCAACTGCAATTAGCTACTCAACCTATTGTGGGTGTTAGTGTCGAACTGCATGACCAGGACGGTCTGTTCCACATTAAAGCCGGTGTTGAATTAGCGATTACGGGCGTGGACCAAACCACAGCGCAAACAGTCATTACGGCAGCGCATGCCATGTGTCCGTTTAGCCGTTTAATTAAACCAGAAAACTTCTTGGGTTTAACCTTGAACGGTGCCAAGCTTTAA", "aa": "MAVIYKTTAHASAGREGVVQTVDGFTVSLAFPKPGATHQDKNNPEQLFASAYAGCFSQAVRVVLQQHQLQLATQPIVGVSVELHDQDGLFHIKAGVELAITGVDQTTAQTVITAAHAMCPFSRLIKPENFLGLTLNGAKL", "aa_hexdigest": "b94a5980c1a9ce9401afd5a7f15cdac8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139829", "ncbi_nrp_id": "WP_010875025.1", "uniref100_id": "UniRef100_P75123", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139829", "RefSeq:WP_010875025.1", "UniRef:UniRef100_P75123" ] }, "ips": { "uniref100_id": "UniRef100_P75123", "uniref90_id": "UniRef90_P75123" }, "psc": { "uniref90_id": "UniRef90_P75123", "gene": "OsmC", "product": "Organic hydroperoxide resistance protein-like", "ec_ids": [ "1.11.1.28" ], "uniref50_id": "UniRef50_P47692", "cog_id": "COG1764", "cog_category": "V", "kegg_orthology_id": "K04063", "go_ids": [ "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_P47692", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47692" ], "product": "Organic hydroperoxide resistance protein-like" }, "genes": [ "osmC" ], "id": "GIHJHMOALN_1411", "locus": "GCA009939745_01411" }, { "type": "cds", "sequence": "contig_1", "start": 793522, "stop": 794721, "strand": "+", "frame": 1, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "db_xrefs": [ "COG:COG0162", "COG:J", "EC:6.1.1.1", "GO:0000166", "GO:0003723", "GO:0004831", "GO:0005524", "GO:0005829", "GO:0006437", "KEGG:K01866", "RefSeq:WP_014325672.1", "SO:0001217", "UniParc:UPI0001DD648B", "UniRef:UniRef100_A0AAV5NAV1", "UniRef:UniRef50_P47693", "UniRef:UniRef90_P75122" ], "nt": "ATGACAAAAGATCTCCTAGCACTTTTAAAAGAACGGGGCTTATTTGTACAAGCTAACTTTGAAAAGGAATTAAAGCAATTATTAAACCAAGGATCATTTGCTTTCTATGTTGGCTTTGACCCCACAGCACCCTCACTCCATATTGGCAACTATGTGCTGCTCCATGTAGCACAAATCTTCCAAGCAATGGGTCATATTCCCCACGTCTTGTTGGGTAGTGGGACGGCTTTAATAGGTGATCCTACTGGCAGAATGGAACTGCGCCAGATGATGAGTCGTGAAACAATTGCCGAAAATACGCGCAACATTAAAAAACAAATTAGGCGCTTTTTGGGTAGTAATGTAGTGTTTTGCCAAAACGAGACCTGGTTAAAGAAACTCAATTACATTGAGGTCATCAGGGAATTGGGACCCTGCTTTTCGGTCAACAAGATGTTAGCGACCGATGCCTTTAGTGCTCGTTGGGAACGCGGTTTAACGTTGATGGAATTGAACTACATGGTATTACAGGCCTATGACTTCTATTATTTAAACCAAAAGTATGGGGTGCAACTGCAAATAGGGGGCAGTGACCAGTGGGCTAATATCCTAGCGGGGGCTGATTTAATTAGACGCAAAACCCAAAAACAGGTGTACGGCATGACCACTAACTTATTAGTGAAAGCTAATGGGGAAAAGATGGGTAAGAGTGCTAGTGGAGCTTTATGGTTAGATCCACAAAAGACCAGTCCGTATGACTTCTACCAGTATTGGATTAACCTTGATGATGCTAGTTTGCAAAAGGTTTTCCTTATGCTCACTAAATTAGATACCAAGGCAATTGAAACCCTCTGCAACTTGAAGGGCGCTGCCATTAAGGAGGCCAAGGCAAAACTAGCCTTCGAGTTAACTGACGCTATTCATGGTACCAAGGCAGCTTTAGCCGCCCAAGCGAAAAGTGCCCGAATCTTTGCCTTTCAACCTGATACTGAAACCAAAACCGTGCGAGCTGGCACCAGGTTAGTGGATGTGATTGTTGATTTAGGTTTAGTTGTATCCAGATCAGAAGCGCGCCGGGTGATTCAGCAAGGTGGTTTAACGATTAACCAAGAAAAAGTGACTGATGTGGAAATGGTCCTACAAGCGAGTTCCCAACCACTAGTGATTGGTAAGGGCAAAAAGCGTTTTGTAACGGTGCAAGTGATAGCTAACACCAAATAA", "aa": "MTKDLLALLKERGLFVQANFEKELKQLLNQGSFAFYVGFDPTAPSLHIGNYVLLHVAQIFQAMGHIPHVLLGSGTALIGDPTGRMELRQMMSRETIAENTRNIKKQIRRFLGSNVVFCQNETWLKKLNYIEVIRELGPCFSVNKMLATDAFSARWERGLTLMELNYMVLQAYDFYYLNQKYGVQLQIGGSDQWANILAGADLIRRKTQKQVYGMTTNLLVKANGEKMGKSASGALWLDPQKTSPYDFYQYWINLDDASLQKVFLMLTKLDTKAIETLCNLKGAAIKEAKAKLAFELTDAIHGTKAALAAQAKSARIFAFQPDTETKTVRAGTRLVDVIVDLGLVVSRSEARRVIQQGGLTINQEKVTDVEMVLQASSQPLVIGKGKKRFVTVQVIANTK", "aa_hexdigest": "1cb0a501a91970a1ead4204c400ce684", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD648B", "ncbi_nrp_id": "WP_014325672.1", "uniref100_id": "UniRef100_A0AAV5NAV1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD648B", "RefSeq:WP_014325672.1", "UniRef:UniRef100_A0AAV5NAV1" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5NAV1", "uniref90_id": "UniRef90_P75122" }, "psc": { "uniref90_id": "UniRef90_P75122", "gene": "tyrS", "product": "tyrosine--tRNA ligase", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_P47693", "cog_id": "COG0162", "cog_category": "J", "kegg_orthology_id": "K01866", "go_ids": [ "GO:0000166", "GO:0003723", "GO:0004831", "GO:0005524", "GO:0005829", "GO:0006437" ] }, "pscc": { "uniref50_id": "UniRef50_P47693", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47693" ], "product": "Tyrosine--tRNA ligase" }, "genes": [ "tyrS" ], "id": "GIHJHMOALN_1412", "locus": "GCA009939745_01412" }, { "type": "cds", "sequence": "contig_1", "start": 794761, "stop": 795798, "strand": "-", "frame": 1, "gene": null, "product": "putative MG456-like protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010875027.1", "SO:0001217", "UniParc:UPI0000139830", "UniRef:UniRef100_P75121", "UniRef:UniRef50_P47694", "UniRef:UniRef90_P75121" ], "nt": "ATGCCAGAGCTAACTCGTTTTCAAAAGTTCTTTTTAACACCTGAAAAGTTCAATAAATTTACAAGAGTTGTTGGTTTTTGTGGTGTCTTTGCTTTAATTGCCCTTTCTTTAGGGATTTACAGCTATGTTGGTCAGGGTTCAATTGTTCCTAAAGTGGCAGCTCTTTTCCTAATTGCCTTAGGCGGGTTTACCTTGCTGTTGAGCTTTGTGATTAACTTTGTAGCTTTGTACAAACGCAGTCAGTTAATTCATTTGGTCAACCGTCAAGATCGTACTGATTTGTGGTTACAAAAGATGGCAAACAACAAGCAGTTTGAGCAATTTGAATTGTTTGAAAAAGGTCCAATTAGTGCGGATATTCTACCGACTTTTTACCCTGCTACCATCTATAACTTTGAGTTAGTTCCCAAGCAATTTAAGGTACAGTACAAGAATGGTCAAACGCTTAACTTTGCTAAGTTAAGTGCCATTAAGAGAAGTACTTCCAAAAATGAAAAGGTGGCTTGCTTAGTCGCTATTATTGATGCTGTTAGTGACCAACACTGGTTCTTAACTAAAAGCGATTTCCCCTTAATTAACACTGGTTTTTACGAATCTTTAACTGAAAGTAACCGCCAGAACAACGTTTTGCTCTACACTGAAAAAGACGCTAGTTTTAACTTTAATCAGTTAGATAAGGAGATGATAAAGCAGGTGTTGTTTAACCCGGTTAATGTGTATGCTAACTTTAACGTTTACAACAATACCACACACACTTACCTCATGATGAGTGTACCGATTACCTTCATGGATACTTCTTTAAGAATGGAGGAAGCTGTGGGTGATTTAGAACTCAATATTACCCGTCAAGCTGGTTATGACGCTGCTACCTTAGATTCATTCCATAAGGTAGTGGAACTGTTAAAGACCAAACTCATTGGTGATTTCAACAACGCTGAAACTACCAGTGCAACAGAAACAACAGTTGTGGCTGAAGTTACTGAACCCACAACCAATTCCAAGCGCAAACCAGTTAAAGCGAAGAAGGCTAAGAAATAA", "aa": "MPELTRFQKFFLTPEKFNKFTRVVGFCGVFALIALSLGIYSYVGQGSIVPKVAALFLIALGGFTLLLSFVINFVALYKRSQLIHLVNRQDRTDLWLQKMANNKQFEQFELFEKGPISADILPTFYPATIYNFELVPKQFKVQYKNGQTLNFAKLSAIKRSTSKNEKVACLVAIIDAVSDQHWFLTKSDFPLINTGFYESLTESNRQNNVLLYTEKDASFNFNQLDKEMIKQVLFNPVNVYANFNVYNNTTHTYLMMSVPITFMDTSLRMEEAVGDLELNITRQAGYDAATLDSFHKVVELLKTKLIGDFNNAETTSATETTVVAEVTEPTTNSKRKPVKAKKAKK", "aa_hexdigest": "5c92a724c8cf4ee52878013d9ef9b424", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139830", "ncbi_nrp_id": "WP_010875027.1", "uniref100_id": "UniRef100_P75121", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139830", "RefSeq:WP_010875027.1", "UniRef:UniRef100_P75121" ] }, "ips": { "uniref100_id": "UniRef100_P75121", "uniref90_id": "UniRef90_P75121" }, "psc": { "uniref90_id": "UniRef90_P75121", "product": "Uncharacterized protein MG456 homolog", "uniref50_id": "UniRef50_P47694", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47694", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47694" ], "product": "Uncharacterized protein MG456" }, "genes": [], "id": "GIHJHMOALN_1413", "locus": "GCA009939745_01413" }, { "type": "cds", "sequence": "contig_1", "start": 795786, "stop": 796367, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014575052.1", "SO:0001217", "UniParc:UPI0001DD648C", "UniRef:UniRef100_A0AAX0SQR6", "UniRef:UniRef50_A0AAX0SQR6", "UniRef:UniRef90_A0AAX0SQR6" ], "nt": "GTGAAACAAACAGTTAAGTTTGCTAGTTTTGTCGATCAGCTCCTAGTTAACCTCGCGCACTTGCAAAAGCAGCTCAACCACTTGCAACCCGATTTGGGCACCTATAGCTTTCAGTGTTTTACCACCCTCAACGATGTTGGTAAAACCCCGCAAAACTTTATTGCAGTCTTAAAGCAACACTCCAACTTGTTGGGTTGTGAAACCAATGCTGTGCTAACTTGGTGGGAGGCTGTAGAATACCTGTTGTTAGAAGAAAACAAGGATGTGATTACCGTGGTGAAAACCCTTGTTAAACACACCGAAAAATTGATACAAACTAAAGCAATTACTTTAGGTCACAAACGGGTGTGGGATTTGTTGTATTACTTTTGTAAAAGCTTCTTGCAATTAGTAAACCGCACCAAAAGGCAGTACAAGCAGCTCGATGGGGTGTTAGTTGAACTGTATGAACGGCTTTGTCAAACCCGGGCGCAGTTTGGCAAGGCGCAGTTTTTAGGGAAACGTTCTGTAGGGCAAACAACTCCAGCGTTGTGTTTAACTTATCTTTTCTTACAATCTTTTATATACGCATGCCAGAGCTAA", "aa": "MKQTVKFASFVDQLLVNLAHLQKQLNHLQPDLGTYSFQCFTTLNDVGKTPQNFIAVLKQHSNLLGCETNAVLTWWEAVEYLLLEENKDVITVVKTLVKHTEKLIQTKAITLGHKRVWDLLYYFCKSFLQLVNRTKRQYKQLDGVLVELYERLCQTRAQFGKAQFLGKRSVGQTTPALCLTYLFLQSFIYACQS", "aa_hexdigest": "79a6a66b652dc4e83b473ee39b988084", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD648C", "ncbi_nrp_id": "WP_014575052.1", "uniref100_id": "UniRef100_A0AAX0SQR6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD648C", "RefSeq:WP_014575052.1", "UniRef:UniRef100_A0AAX0SQR6" ] }, "ips": { "uniref100_id": "UniRef100_A0AAX0SQR6", "uniref90_id": "UniRef90_A0AAX0SQR6" }, "psc": { "uniref90_id": "UniRef90_A0AAX0SQR6", "uniref50_id": "UniRef50_A0AAX0SQR6" }, "pscc": { "uniref50_id": "UniRef50_A0AAX0SQR6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX0SQR6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22313.758100000003, "isoelectric_point": 9.203845024108887 }, "id": "GIHJHMOALN_1414", "locus": "GCA009939745_01414" }, { "type": "cds", "sequence": "contig_1", "start": 796351, "stop": 797808, "strand": "-", "frame": 1, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357JCK8" ], "nt": "GTGAGGTTTGACAACATTGCGGGACTCCAAGAAGAAAAACACGAGTTACTGGAAATTGTTGATTACCTCAAAAACCCCTTGAAGTATGCCCAAATGGGCGCTCGTTCCCCACGCGGTGTTATTCTTTACGGCCCTCCTGGTACGGGTAAAACGCTGTTAGCGAAAGCTGTTGCTGGTGAAGCTGGTGTGCCTTTCTTCCAGTCCACGGGTTCTGGCTTTGAAGATATGCTGGTTGGTGTGGGCGCCAAACGGGTACGGGACTTGTTCAATAAGGCTAAGAAAGCGGCCCCATGTATTATCTTCATCGATGAAATTGACTCGGTTGGTTCTAAACGGGGTCGCGTGGAGTTGTCCTCCTACTCGGTGGTCGAACAAACCTTAAACCAACTGTTAGCGGAAATGGATGGGTTTACTAGCCGCACTGGTGTTGTGGTGATGGCTGCTACCAACCGCTTGGATGTCCTTGATGATGCGTTATTACGCCCAGGTCGGTTTGATCGTCACATCCAGATTAACCTTCCCGACATTAAGGAACGCGAAGGCATCTTACAAGTGCACGCCAAGAACAAGAACTTGTCTTCCAAGATTAGCTTATTGGATGTTGCTAAACGTACCCCTGGTTTTAGTGGGGCTCAATTGGAAAACGTCATTAATGAAGCTACCCTGTTAGCAGTCCGTGATAACCGGACCACGATCAACATGAACGACATTGATGAAGCCATTGACCGGGTCATAGCTGGTCCTGCCAAAAAATCTCGGGTGGTCAGTGATGCTGATCGTAAACTAGTGGCCTATCACGAAGCAGGTCATGCTTTGGTTGGCCTCCATGTTCACAGTAACGATGAAGTGCAAAAGATTACCATTATTCCCCGGGGTCAAGCGGGTGGTTATACCTTGTCAACCCCTAAGAGTGGCGATCTCAATTTAAAGCGTAAATCTGACTTGTTAGCGATGATTGCTACAGCCATGGGCGGCCGTGCTGCAGAGGAAGAAATTTACGGTCCCCTAGAAATTACCACTGGCGCTTCCTCGGACTTTTATAAGGCGACCAATATTGCCCGTGCCATGGTTACCCAGTTAGGAATGTCCAAGTTAGGGCAAGTGCAATATGTACCTAGTCAAGGTACCGTCCCACCAGGTACCAAACTGTTCTCTGAGCAAACAGCAAAGGATATTGACTTTGAGATTAACGCCATTATTGAAGAACAGTACAAGAAGGCACGTACCATTATTAAAACGAACCGTAAGGAGTTGGAATTGTTAGTTGAAGCCTTGTTGATTGCCGAAACCATCTTAAAGAGTGACATCGATTACATTCACGAACACACTAAACTCCCTCCAGAGATCCTAGCGCAAAAGCAAGAACAGCAAGCCAAACAAAAGGCAGAAGCCAAGGAAGCTAAGTTAAATAAGAAGACTGAGAAGGATACAGAAAAAGACAGTGAAACAAACAGTTAA", "aa": "MRFDNIAGLQEEKHELLEIVDYLKNPLKYAQMGARSPRGVILYGPPGTGKTLLAKAVAGEAGVPFFQSTGSGFEDMLVGVGAKRVRDLFNKAKKAAPCIIFIDEIDSVGSKRGRVELSSYSVVEQTLNQLLAEMDGFTSRTGVVVMAATNRLDVLDDALLRPGRFDRHIQINLPDIKEREGILQVHAKNKNLSSKISLLDVAKRTPGFSGAQLENVINEATLLAVRDNRTTINMNDIDEAIDRVIAGPAKKSRVVSDADRKLVAYHEAGHALVGLHVHSNDEVQKITIIPRGQAGGYTLSTPKSGDLNLKRKSDLLAMIATAMGGRAAEEEIYGPLEITTGASSDFYKATNIARAMVTQLGMSKLGQVQYVPSQGTVPPGTKLFSEQTAKDIDFEINAIIEEQYKKARTIIKTNRKELELLVEALLIAETILKSDIDYIHEHTKLPPEILAQKQEQQAKQKAEAKEAKLNKKTEKDTEKDSETNS", "aa_hexdigest": "16d13bd606ae783af3b7d49c732ab51c", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5RJK9", "query_cov": 0.9237113402061856, "subject_cov": 0.8327137546468402, "identity": 0.5579999999999999, "score": 473.0, "evalue": 3.3e-160, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A357JCK8", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A357JCK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357JCK8" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "GIHJHMOALN_1415", "locus": "GCA009939745_01415" }, { "type": "cds", "sequence": "contig_1", "start": 797902, "stop": 798393, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTATCCTAGCCATTATTATTGGTATTTTGGTTTATATCCTAATGCCAAGGGCTACCACGGCTGTAATTGAAAAGTGGGAGCTTAGTGGAACTACACTTTCGGCTCAAATCAAAGGCTTATCAGGCAAACACACCTTCCAAAGAATTAATAATTCCACCTATGTCACCGATGACATTCTGCAGGTTTCCATTAGTTTCCAAGGGATAAATCCCATAGTAGTAACTGCCCACAAAGCTACTAATGGTAGTGGGGAAACTATCTTTAATATAGCAAACCTCTCGATTAATCAGTCAACTGGAAAGGCCATAGTTAATGGAATGATGACCCAAGACCAAAAAAGTAACAATGGCACTGAATTAGCTTCTATTAAAGGTCTCCATGACATCGGTACCTTTGTTGCTCCTGATACGAGAGCACGTGATGTGCTCAACATCTTCTTTGGCCTACTACCAATTATTATCTTTGTCATTTTCTTCTTACTCTTTTGA", "aa": "MVILAIIIGILVYILMPRATTAVIEKWELSGTTLSAQIKGLSGKHTFQRINNSTYVTDDILQVSISFQGINPIVVTAHKATNGSGETIFNIANLSINQSTGKAIVNGMMTQDQKSNNGTELASIKGLHDIGTFVAPDTRARDVLNIFFGLLPIIIFVIFFLLF", "aa_hexdigest": "fe17ecc576f14c70095f23a16dbcdfbf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17709.454599999994, "isoelectric_point": 8.068298912048338 }, "id": "GIHJHMOALN_1416", "locus": "GCA009939745_01416" }, { "type": "cds", "sequence": "contig_1", "start": 798467, "stop": 798640, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD648D", "UniRef:UniRef100_A0AB38W6Q2", "UniRef:UniRef50_A0AB38W6Q2", "UniRef:UniRef90_A0AB38W6Q2" ], "nt": "ATGCGATGGTTTAGTGTGTTGGTTTCCCTGGTAAAAAAGCACAAAAAAATCATCTTAATTACCTTTCTAACTTTGGCTGGTTTTGCAGCAGTGGGTGTATTAGTTACCCTTAGTGTTGTTAGAATAAAAACCAGTCAAATCACCCAACAAACAACAACAAATGAAAAAAAATAA", "aa": "MRWFSVLVSLVKKHKKIILITFLTLAGFAAVGVLVTLSVVRIKTSQITQQTTTNEKK", "aa_hexdigest": "b69880654c72a7dc3444d60a213cf24e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD648D", "uniref100_id": "UniRef100_A0AB38W6Q2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD648D", "UniRef:UniRef100_A0AB38W6Q2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W6Q2", "uniref90_id": "UniRef90_A0AB38W6Q2" }, "psc": { "uniref90_id": "UniRef90_A0AB38W6Q2", "uniref50_id": "UniRef50_A0AB38W6Q2" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W6Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W6Q2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6386.6795, "isoelectric_point": 11.30106601715088 }, "id": "GIHJHMOALN_1417", "locus": "GCA009939745_01417" }, { "type": "cds", "sequence": "contig_1", "start": 798661, "stop": 799188, "strand": "-", "frame": 1, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0634", "COG:F", "EC:2.4.2.8", "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657", "KEGG:K00760", "RefSeq:WP_014325673.1", "SO:0001217", "UniParc:UPI0001DD648E", "UniRef:UniRef100_A0AAV5N6V8", "UniRef:UniRef50_P47696", "UniRef:UniRef90_P75119" ], "nt": "ATGGGCATTAAATCTATCATCATTGATCAAAAACAGGTTGAAGCGGGTTGTAACGCTGCGCTCAAATGGTGTAATGAACATTTTGCTGGTAAACAGGTCATAGTATTAGGGATCTTGAAGGGTTGTATTCCCTTTTTGGGCAAACTTATTAGTCAGTTTACCTTTGATTTACAACTGGACTTTGTGGCAGTCGCTTCTTACCATGGTGGCAGTCGCCAACAAGAAGCACCGAAGATTGTCCTAGATATGTCCCATGACCCTAAAGGCAAGGACATTCTTTTAATAGAAGACATAATCGACAGTGGTCGTTCTATTAAATTAGTATTGGACTTACTCCACACCCGAAAGGCCAAAAGTGTGATCTTAGTGAGCTTCATTGAAAAGCTCAAACCCAGGGAAGCTGACATTAAGGTGGACTATTCTTGCTTTAAAAACCAAGACGAATTCTTGGTTGGTTTTGGGTTAGACTACCAGGGCTTTTATCGCAATTTACCCTATGTTGGTGTCTTTGATCCTGAAGATAATTAA", "aa": "MGIKSIIIDQKQVEAGCNAALKWCNEHFAGKQVIVLGILKGCIPFLGKLISQFTFDLQLDFVAVASYHGGSRQQEAPKIVLDMSHDPKGKDILLIEDIIDSGRSIKLVLDLLHTRKAKSVILVSFIEKLKPREADIKVDYSCFKNQDEFLVGFGLDYQGFYRNLPYVGVFDPEDN", "aa_hexdigest": "b7160731d2072f4fbc2daf194a054ec1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD648E", "ncbi_nrp_id": "WP_014325673.1", "uniref100_id": "UniRef100_A0AAV5N6V8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD648E", "RefSeq:WP_014325673.1", "UniRef:UniRef100_A0AAV5N6V8" ] }, "ips": { "uniref100_id": "UniRef100_A0AAV5N6V8", "uniref90_id": "UniRef90_P75119" }, "psc": { "uniref90_id": "UniRef90_P75119", "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_P47696", "cog_id": "COG0634", "cog_category": "F", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657" ] }, "pscc": { "uniref50_id": "UniRef50_P47696", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47696" ], "product": "Hypoxanthine-guanine phosphoribosyltransferase" }, "genes": [ "hpt" ], "id": "GIHJHMOALN_1418", "locus": "GCA009939745_01418" }, { "type": "cds", "sequence": "contig_1", "start": 799188, "stop": 799613, "strand": "-", "frame": 2, "gene": "ispF", "product": "putative MG459-like protein", "db_xrefs": [ "COG:COG0245", "COG:I", "GO:0008685", "GO:0016114", "SO:0001217", "UniRef:UniRef50_Q49436", "UniRef:UniRef90_P75118" ], "nt": "GTGGAAATAGAAGATTCCAAGTACATTTATGACCACGATGCTAGTGATGACGCCAGTGACGTTATCCAGCTAGCAGTAGCTGATGCTTTATTTGGTGCTACTGCCTTAGGTGATGGACAAATTGTCTTCAATAAAGAAAAAACCCAGATCCCTTTAAAGGGTAATCCGCGTAAAGAAGCGCCCCGTATTTTGGCACGGACTTACAACTTTATTCGCAAGAACTGGTACATCAATAACATTGATATCACCTTAGAAATTCCCTCACAACAAAAGATGGATGACTATAAGCATGCCATCTTTGACTTTATTTGTACGGCTTTACGGATTACCGAACTGACAATTAACCTCAAGGTACGTGAACCGTTGAATTCGAATGAAATTAGTTGTTTAGCGGTCGTGTTAGTGGAACGACAAAGATTAAAGTAA", "aa": "MEIEDSKYIYDHDASDDASDVIQLAVADALFGATALGDGQIVFNKEKTQIPLKGNPRKEAPRILARTYNFIRKNWYINNIDITLEIPSQQKMDDYKHAIFDFICTALRITELTINLKVREPLNSNEISCLAVVLVERQRLK", "aa_hexdigest": "c6853fe3a877d1c2bec26b1e72d9a64f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75118", "query_cov": 1.0, "subject_cov": 0.834319526627219, "identity": 0.986, "score": 275.0, "evalue": 2.68e-93, "valid": true, "gene": "IspF", "product": "Uncharacterized protein MG459 homolog", "uniref50_id": "UniRef50_Q49436", "cog_id": "COG0245", "cog_category": "I", "go_ids": [ "GO:0008685", "GO:0016114" ] }, "pscc": { "uniref50_id": "UniRef50_Q49436", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49436" ], "product": "Uncharacterized protein MG459" }, "genes": [ "ispF" ], "id": "GIHJHMOALN_1419", "locus": "GCA009939745_01419" }, { "type": "cds", "sequence": "contig_1", "start": 799815, "stop": 800807, "strand": "+", "frame": 3, "gene": "ldh", "product": "L-lactate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0039", "EC:1.1.1.27", "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006096", "GO:0016020", "KEGG:K00016", "SO:0001217", "UniParc:UPI0001DD6490", "UniRef:UniRef100_P78007", "UniRef:UniRef50_P78007", "UniRef:UniRef90_P78007" ], "nt": "ATGATTGGTGGCTTAAACCAAATTAAAATGAAGGCAAGCTTGAATAAATTAGTTATGAAGAGTCTTAAAGTAGCACTCATTGGTTCTGGTGCAGTGGGCACCAGCTTTCTCTACGCTGCCATGAGCCGTGGTTTAGCGAGTGAATACATGGTTATCGACATTAACGAAAAATCCCAAGTAGGGAATGTTTTTGACCTCCAAGATGCTGTACCATCATCACCGCAATACAGTAAGGTAATTGCTGGTGATTACAAACAACTCAAAGATTACGACTTTATCTTTATTGGTGCTGGTCGACCCCAAAAGCAAGGTGGGGAAACCCGGTTACAACTCCTTGAAGGTAATGTCGAAATTATGAAGAACATTGCCAAAGCCGTGAAGGAATCGGGCTTTAAGGGCATTACCTTAATTGCTTCCAACCCAGTCGACATTATGGCTTACACCTATTTAAAGGTAACGGGGTTTGAACCGAATAAGGTAATTGGTTCTGGAACCTTACTAGACAGCGCCCGGTTAAAGTTTGCGATTGCTGAAAAATACGGTATGAGCAGCAGGGATGTCCAAGCATATGTGTTAGGTGAACACGGGGATAGTTCAGTAAGTATTATTTCCTCCGCTAAGATTGCTGGCTTACCACTCAAACACTTCTCGAAAGCAAGTGATATAGAAAAAGAATTTGCTGAAATTGACCACTTTATTCGCCGTCGAGCTTATGAAATCATCGAACGCAAAGGAGCCACCTTTTATGGCATCGGGGAAGCAACTGCCGAAGTAGCAGAATTAATTCTCCGCGACACTAAAGAAGTACGAGTAGTAGCATCGTTAATTAATGGCCAATATGGTGCTAAAGATGTAATGTTTGGCACCCCTTGTGTCTTGGGACGCAATGGTGTGGAAAAGATCTTGGAAATTGAATTGTCTGCTACAGAAAAAGCAGGCTTGGACAAATCAATCCAAGTATTGAAGGACAACATTAAGTTAGCTAAACTATAA", "aa": "MIGGLNQIKMKASLNKLVMKSLKVALIGSGAVGTSFLYAAMSRGLASEYMVIDINEKSQVGNVFDLQDAVPSSPQYSKVIAGDYKQLKDYDFIFIGAGRPQKQGGETRLQLLEGNVEIMKNIAKAVKESGFKGITLIASNPVDIMAYTYLKVTGFEPNKVIGSGTLLDSARLKFAIAEKYGMSSRDVQAYVLGEHGDSSVSIISSAKIAGLPLKHFSKASDIEKEFAEIDHFIRRRAYEIIERKGATFYGIGEATAEVAELILRDTKEVRVVASLINGQYGAKDVMFGTPCVLGRNGVEKILEIELSATEKAGLDKSIQVLKDNIKLAKL", "aa_hexdigest": "853c4494060764869b93776d963abaf6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD6490", "uniref100_id": "UniRef100_P78007", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD6490", "UniRef:UniRef100_P78007" ] }, "ips": { "uniref100_id": "UniRef100_P78007", "uniref90_id": "UniRef90_P78007" }, "psc": { "uniref90_id": "UniRef90_P78007", "gene": "ldh", "product": "L-lactate dehydrogenase", "ec_ids": [ "1.1.1.27" ], "uniref50_id": "UniRef50_P78007", "cog_id": "COG0039", "cog_category": "C", "kegg_orthology_id": "K00016", "go_ids": [ "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006096", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P78007", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78007" ], "product": "L-lactate dehydrogenase" }, "genes": [ "ldh" ], "id": "GIHJHMOALN_1420", "locus": "GCA009939745_01420" }, { "type": "cds", "sequence": "contig_1", "start": 801212, "stop": 801517, "strand": "-", "frame": 3, "gene": null, "product": "UPF0134 protein MPN_675", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AFE3", "UniRef:UniRef100_P75117", "UniRef:UniRef50_P75076", "UniRef:UniRef90_P75117" ], "nt": "ATGGGCTTTTATGGCAATTTAAACCATATGGAAAAAGGCAAAAGCGGATACGTAACCCATAAACAATTGGACAAAAAACTAGAAGTTTTTAAACAAGAGCTTTTAGTTGAGTTTGATCAAAGATATGTTACTAAGGCAGAATTTAAAGAATTCCAAATAGAAGTTAGAGAAGGCTTTCGAATTCAGGGTGAAGCAATGAACGCCCGCATGGATCGCTTTGAAAGTCTTGTGCTTAAAAGCCTGGAATCAATTAACAATACCTTAATAGACTTTGGCAAACGTATAGACAAATTGGAATCTAAATAA", "aa": "MGFYGNLNHMEKGKSGYVTHKQLDKKLEVFKQELLVEFDQRYVTKAEFKEFQIEVREGFRIQGEAMNARMDRFESLVLKSLESINNTLIDFGKRIDKLESK", "aa_hexdigest": "71ea43af843ad1e29008d9c7697ae0cf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AFE3", "uniref100_id": "UniRef100_P75117", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AFE3", "UniRef:UniRef100_P75117" ] }, "ips": { "uniref100_id": "UniRef100_P75117", "uniref90_id": "UniRef90_P75117" }, "psc": { "uniref90_id": "UniRef90_P75117", "product": "UPF0134 protein MPN_675", "uniref50_id": "UniRef50_P75076" }, "pscc": { "uniref50_id": "UniRef50_P75076", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75076" ], "product": "UPF0134 protein MPN_038" }, "genes": [], "id": "GIHJHMOALN_1421", "locus": "GCA009939745_01421" }, { "type": "cds", "sequence": "contig_1", "start": 801871, "stop": 802008, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003684E99", "UniRef:UniRef100_A0AB38W5S1", "UniRef:UniRef50_A0AB38W5S1", "UniRef:UniRef90_A0AB38W5S1" ], "nt": "ATGGTTGATCGAAGGTTCGCAAACATTGAAGCGGAGTTGAGAGACTTCAAAGTAGAAGTTAGAGAAACTTTTAAAGTTGTGCTTCAGAGCCTTCAAGCCATCAACGAACGTTTAGATAGGCTTGAATACAAGAAATAA", "aa": "MVDRRFANIEAELRDFKVEVRETFKVVLQSLQAINERLDRLEYKK", "aa_hexdigest": "4a5fd6605f621f829c04fd9def02ed38", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003684E99", "uniref100_id": "UniRef100_A0AB38W5S1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003684E99", "UniRef:UniRef100_A0AB38W5S1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB38W5S1", "uniref90_id": "UniRef90_A0AB38W5S1" }, "psc": { "uniref90_id": "UniRef90_A0AB38W5S1", "uniref50_id": "UniRef50_A0AB38W5S1" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W5S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W5S1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5497.2922, "isoelectric_point": 8.1404390335083 }, "id": "GIHJHMOALN_1422", "locus": "GCA009939745_01422" }, { "type": "cds", "sequence": "contig_1", "start": 802785, "stop": 802898, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACAAGCACACTAACCTCAGTGAAATCAGTATGTTGGTAAAGCGTAGTAATGAACTGTTCTTTGAAAATGCACTGTACAAGTGGGCTAACGTTTTCTACCGCCTTTAA", "aa": "MQNKHTNLSEISMLVKRSNELFFENALYKWANVFYRL", "aa_hexdigest": "8b6d8dbb7a728f17c886c7e3bc5f6e96", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4536.196000000001, "isoelectric_point": 9.397636985778806 }, "id": "GIHJHMOALN_1423", "locus": "GCA009939745_01423" }, { "type": "cds", "sequence": "contig_1", "start": 802932, "stop": 804062, "strand": "-", "frame": 2, "gene": "ydhJ", "product": "putative MG461-like protein", "db_xrefs": [ "COG:COG1078", "COG:R", "GO:0006203", "GO:0008832", "SO:0001217", "UniRef:UniRef50_P47699", "UniRef:UniRef90_P75115" ], "nt": "ATGCAACAAATCTTTTTTAAAGATCCCATTTTAGGGGAAGTGCTGTTTGACCAACAAACTAAGTGGATGTATGAGTTGGTGGAAACGGAAGCGTTTCGCCGTTTACGTAACATTAAGCAGCTAGGAATTAACTTTCATTTTTATCCGGGGGGCGTGCACACGCGTTATTCCCACTCCTTGGGGGTTTATGAACTGTTAAGGCGGATCTTAAACACCCCCGCGTTTGCTCCGATTAATGAAAACAAAAAGCAAACGGTGTTAGTAGCGGGATTACTGCACGACATTGGTCATGCACCGCATTCCCATGCCTTTGAGATTTACTTTGCCAAAGCCCCTAACTTTAAGAAGGAGCTGTTTATCCACGAAGAAGTTACAACCTTATTTGTCAACAGTGAACCGATTAAATCAATCTTAAAGGCGAACCAGATTGATCCTAAACTAGTAGCTGCTTTAATTGATGAAAACAAGGAGTTAAAGCCATCCAACTACTGGATGCGCCAGTTAATTTCATCTGACTTGGATGCTGACCGGATGGATTACCTGTTGCGCGATTCCTACTTTACGGGTACCAGTCATTCCTTGATAGATTACCAAACGATTATTAAGGAGATGGACTGTGTCAAGGTCAAGGGGATTTACGAAATCTTCTTTAAGGATAAGTGCTTGCCCTTAATTGAAAACTTCTTAATTACCCGGCACCACATGTACCAATCAATCTACAGCGATGGCAGGAGTATTTCCACCGAGTTAAACCTTTGGTTTGTCTTTCAACGAATTAAGGACTTAGTGGACAAAAACCAGTTTGACTTCAACGGTTATAAAACCTTAGAACAGGTGTGCTTACCACTACTCAAGAACGAACACTTTGACAAAAAGATGCTGCCAGCGTTTGTTAAGTTAGATGACTATGTGTTCCAATCGTTCTTTGTTAACTTGTACCAAACCACCAAGGACAAGATCTTAAAGAAGTTGTTGGACAGTTATTTAAACAGTTTGAAGTTCGAAATTAAGTTTTACGAAACCAAGGAACAGAGGGACTTAGACTTTGAAAAACAGGCGAGCAAGTACAAGGATGCTAAGTACTTTATTACGAAGTTTAACAATCAGTTTAAGGGCTTTTATGAGGGTTGA", "aa": "MQQIFFKDPILGEVLFDQQTKWMYELVETEAFRRLRNIKQLGINFHFYPGGVHTRYSHSLGVYELLRRILNTPAFAPINENKKQTVLVAGLLHDIGHAPHSHAFEIYFAKAPNFKKELFIHEEVTTLFVNSEPIKSILKANQIDPKLVAALIDENKELKPSNYWMRQLISSDLDADRMDYLLRDSYFTGTSHSLIDYQTIIKEMDCVKVKGIYEIFFKDKCLPLIENFLITRHHMYQSIYSDGRSISTELNLWFVFQRIKDLVDKNQFDFNGYKTLEQVCLPLLKNEHFDKKMLPAFVKLDDYVFQSFFVNLYQTTKDKILKKLLDSYLNSLKFEIKFYETKEQRDLDFEKQASKYKDAKYFITKFNNQFKGFYEG", "aa_hexdigest": "b96947313a171348d4e25d62c23e16ae", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75115", "query_cov": 1.0, "subject_cov": 0.8847058823529412, "identity": 0.997, "score": 759.0, "evalue": 1.08e-276, "valid": true, "gene": "YdhJ", "product": "Uncharacterized protein MG461 homolog", "uniref50_id": "UniRef50_P47699", "cog_id": "COG1078", "cog_category": "R", "go_ids": [ "GO:0008832", "GO:0006203" ] }, "pscc": { "uniref50_id": "UniRef50_P47699", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47699" ], "product": "Uncharacterized protein MG461" }, "genes": [ "ydhJ" ], "id": "GIHJHMOALN_1424", "locus": "GCA009939745_01424" }, { "type": "cds", "sequence": "contig_1", "start": 804043, "stop": 804504, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "TTGAGTGATAATGCCTACTTTAACTTTATTGATAACTACTTAACGATTGACTTTGATTACCTCAAGGACAAGCGCAAAGAAGTCAGTCTGTTGTTCAAAAACCAACTAGCGTTTGGCATTGAAATTAACCAACTGATTAAGGAAACCTTTGCCCCCAAGTTAGGGGTGCAGCATTTAAGTGTTAAGCACCGTGAACTCTTCAAAGAGCTCCAACCAGCCCTACAACAACTGAGTGAACAACTCCAAGCCCTACCTGATTGGACGAAGGACAATGTCAAAAGTACCTTAACCCAAATTGGTGAACAGTTTAATCTGAAGGGCAAAAAGCTGTTTATGCCACTACGGTTGATCTTTACCAACAAAGAGCATGGTCCTGATTTGGCGGGCATCATGGTGCTGCACGGTAAGACTCAGGTATTAGCGCTGCTTCAAGAATTTATTCATGCAACAAATCTTTTTTAA", "aa": "MSDNAYFNFIDNYLTIDFDYLKDKRKEVSLLFKNQLAFGIEINQLIKETFAPKLGVQHLSVKHRELFKELQPALQQLSEQLQALPDWTKDNVKSTLTQIGEQFNLKGKKLFMPLRLIFTNKEHGPDLAGIMVLHGKTQVLALLQEFIHATNLF", "aa_hexdigest": "2021360d80e92d78197fbf0eabac30b4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 128, "aa_cov": 0.8169934640522876, "hmm_cov": 1.0, "evalue": 5.1502985921918427e-20, "score": 67.79653930664062, "start": 22, "stop": 146 } ], "seq_stats": { "molecular_weight": 17717.375899999988, "isoelectric_point": 7.947871971130372 }, "id": "GIHJHMOALN_1425", "locus": "GCA009939745_01425" }, { "type": "cds", "sequence": "contig_1", "start": 804640, "stop": 804939, "strand": "-", "frame": 1, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002849521E" ], "nt": "TTGAAGGCCAATGGCATTGCCACGTATAACTTTGCCGTAGTCATTGATGACCATGACATGGAAATTACCGATGTGTTGCGGGGGGCGGAACACATTTCCAACACCGCTTACCAACTAGCAATTAACCAAGCCTTAGGCTATCAAAGGATTCCCCGCTTTGGTCATTTATCGGTGATAGTCGACAAGAGTGGCAAGAAACTGTCTAAGCGTGATACCAAAACGATCCAGTTCATTGAACAGTTCAAACAAGAGGGTTACCTACCAGAAGCGGTGGTAAACTTCTTGGCCTTACTGGGTTGA", "aa": "MKANGIATYNFAVVIDDHDMEITDVLRGAEHISNTAYQLAINQALGYQRIPRFGHLSVIVDKSGKKLSKRDTKTIQFIEQFKQEGYLPEAVVNFLALLG", "aa_hexdigest": "55a97675094a3aea2b1a3abc8eeb52f4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0037046112", "query_cov": 0.9292929292929293, "subject_cov": 0.8288288288288288, "identity": 0.598, "score": 114.0, "evalue": 3.63e-31, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI002849521E" }, "pscc": { "uniref50_id": "UniRef50_UPI002849521E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002849521E" ], "product": "glutamate--tRNA ligase family protein" }, "genes": [], "id": "GIHJHMOALN_1426", "locus": "GCA009939745_01426" }, { "type": "cds", "sequence": "contig_1", "start": 805000, "stop": 805497, "strand": "-", "frame": 1, "gene": null, "product": "Glutamate--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7" ], "nt": "ATGGAAAAGATTAGAACCCGTTATGCCCCTTCCCCTACCGGTTACCTTCATGTTGGGGGTGCGCGTACTGCTATCTTTAACTTCTTGTTAGCAAAACACTTTAACGGCGAGTTTATTATCCGGATTGAGGACACTGACACTGAGCGCAATGTGGAAGGGGGAATTGAATCACAACTAGAAAACTTGCGCTGGTTAGGAATTATTCCCGATGAATCAATTTACAACCCGGGTAATTATGGGCCTTACATCCAAAGTCAAAAGTTGGCTACCTACAAAAAACTAGCTTATGAACTTGTGGGCAAGGGTTTGGCCTACCGTTGTTTCTGTACTAAAGAAAAGTTAGAACACGAACGCCAATTGGCTTTAGAACACCACCAAACCCCGAAGTATTTGGGAACGTGTCGCAACTTACACTCCAAGCACATTCAAACAAACTTAGACAACCAAGTACCCTTCACGATTCGCTTAAAGATTAACCAGGACGCTGAGTTTGCCTGA", "aa": "MEKIRTRYAPSPTGYLHVGGARTAIFNFLLAKHFNGEFIIRIEDTDTERNVEGGIESQLENLRWLGIIPDESIYNPGNYGPYIQSQKLATYKKLAYELVGKGLAYRCFCTKEKLEHERQLALEHHQTPKYLGTCRNLHSKHIQTNLDNQVPFTIRLKINQDAEFA", "aa_hexdigest": "625e0b899028ac41140e30eaeea8ae4a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A654IDE7", "query_cov": 0.8666666666666667, "subject_cov": 0.9727891156462585, "identity": 0.58, "score": 162.0, "evalue": 2.71e-48, "valid": false, "product": "Glutamate--tRNA ligase", "uniref50_id": "UniRef50_A0A654IDE7" }, "pscc": { "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7" ], "product": "Glutamate--tRNA ligase" }, "genes": [], "id": "GIHJHMOALN_1427", "locus": "GCA009939745_01427" }, { "type": "cds", "sequence": "contig_1", "start": 805484, "stop": 806275, "strand": "-", "frame": 3, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "COG:COG0030", "COG:J", "EC:2.1.1.182", "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0052908", "RefSeq:WP_010875036.1", "SO:0001217", "UniParc:UPI000012E0AF", "UniRef:UniRef100_P75113", "UniRef:UniRef50_P47701", "UniRef:UniRef90_P75113" ], "nt": "GTGAATAGTTTTTATCCTTCACGCAAATTAGGGCAAAACTTTACGGTTGACCAGAGTGTAATTGCCAAAACCTGTCGGTTAATTAAGAGTCTCAACCCAACGGCCTTAATTGAAGTGGGGCCGGGTAAGGGAGCGTTAACCAAGGCTTTACTCAAGTTACAACTACCATACCATGGCATAGAGTTGGATAAACGCTTAGCGGAATACCTCTTGGTCAACGAAATCTTAACTGAAGAACAACTAACCATTGGTGATGCCTTAAAACAAAACTTGGATCAGTATTTTCCTGACACTATACCTTTGCTTTGTGGCAACATTCCCTACAGTATTAGCTCCCCTTTGATTGCTAACTTTTTGGCTTCCAAACTACAACAATTTGTGCTGGTGTGCCAGTGGGAGTTTGGTCAAAGGTTAGTAGCACCGGTAAATTCGCCTAACTATAGTGCCTTTGGTGTGTTTTGCCAATACCATTTGCAAATTAAATCAGTTTTTAAAATTGATAAGGTTGCTTTTAAGCCCAAGCCCCAAGTGGATTCGGTGTTAATGTTGTTAAAAAAGAAACCCCAAGTAGCCTATGAAGCTCATTTTGGCCGGTTCTTAAAACAGTGTTTTCACCAGCGCCGCAAGCTGTTAGTCAATAACCTCAAGCAGCTTCTCCCACCCACCTTGTTAACTAATGTTTTACAACAACAGGACTTGGCGGCAACGGTTAGAGCCCAAGAGTTAACCCCGACCCAGTTGTTTCGTTTGTATCTTAGCCTTAAACCACATTTAAGCGATGGAAAAGATTAG", "aa": "MNSFYPSRKLGQNFTVDQSVIAKTCRLIKSLNPTALIEVGPGKGALTKALLKLQLPYHGIELDKRLAEYLLVNEILTEEQLTIGDALKQNLDQYFPDTIPLLCGNIPYSISSPLIANFLASKLQQFVLVCQWEFGQRLVAPVNSPNYSAFGVFCQYHLQIKSVFKIDKVAFKPKPQVDSVLMLLKKKPQVAYEAHFGRFLKQCFHQRRKLLVNNLKQLLPPTLLTNVLQQQDLAATVRAQELTPTQLFRLYLSLKPHLSDGKD", "aa_hexdigest": "3bd4dccd0ca3a6ffdda6131a30d13600", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E0AF", "ncbi_nrp_id": "WP_010875036.1", "uniref100_id": "UniRef100_P75113", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E0AF", "RefSeq:WP_010875036.1", "UniRef:UniRef100_P75113" ] }, "ips": { "uniref100_id": "UniRef100_P75113", "uniref90_id": "UniRef90_P75113" }, "psc": { "uniref90_id": "UniRef90_P75113", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_P47701", "cog_id": "COG0030", "cog_category": "J", "go_ids": [ "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0052908" ] }, "pscc": { "uniref50_id": "UniRef50_P47701", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47701" ], "product": "Ribosomal RNA small subunit methyltransferase A" }, "genes": [ "rsmA" ], "id": "GIHJHMOALN_1428", "locus": "GCA009939745_01428" }, { "type": "cds", "sequence": "contig_1", "start": 806268, "stop": 806567, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATTTATCTCGCAAAAGTTGCCCCAAATTTGGGCTAGCAAACGCAATGAGAATGCTAAAGCGCATTCGCAAAAGAGCATTGAACAGCTCAATAAAACAAAGCGAATGCAGTTAATTTTCTACTTTGTGTTTGCTGTTATTACGGCTTTTAGTGCCGCTGGTGTGGGTGTGTACTGGTTCTTAAATGCCTTGTTTACCTTACTCCAGTCTTATCTAACCCACCTCTTTATTGTTAAAAGGCGTACCAGAAGACGGTTAACGTATTCCAAATTAGAACAAATGTTAGAACGTGAATAG", "aa": "MQFISQKLPQIWASKRNENAKAHSQKSIEQLNKTKRMQLIFYFVFAVITAFSAAGVGVYWFLNALFTLLQSYLTHLFIVKRRTRRRLTYSKLEQMLERE", "aa_hexdigest": "58fb5a875f8968c7bcd6e65d0c32bcd5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11818.760199999988, "isoelectric_point": 10.620344352722167 }, "id": "GIHJHMOALN_1429", "locus": "GCA009939745_01429" }, { "type": "cds", "sequence": "contig_1", "start": 806601, "stop": 807029, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGCTACCAGGGTTCCTCTAGGTAGCGGTCCGGAATTGGGCTTCAACATGATCTTGTCTTTGATTGTGTTGTTGTTCCTAGTACGGTTAATTACCATAGTGATTACCCTCAACTCCACGCTGGCGTTAGAAAAAATGAACGAGGTCCAGGGCAAATTAGCAGAAATTAATGCCAAGTACAAAGGGGCGTTGGACTTACAGAGCAAACGCAACCGCCAGATGGAGATCATGTCCTTGTACAAGAAGCACAACATTAAGTCTTCTGCTTCCTTTGTTCAGGTCTTTGTTACCTTACCCATCTTCCTCATTATTTACCGGATTGTCACGACTTTAAGACCAATTAAAGCGATTATCCTGTTTAACTTCTGGGACTTATCTAAGGTACCGTTAACGGAAATCTTCTCGAACTTTACCAGTACCGGGTGA", "aa": "MYATRVPLGSGPELGFNMILSLIVLLFLVRLITIVITLNSTLALEKMNEVQGKLAEINAKYKGALDLQSKRNRQMEIMSLYKKHNIKSSASFVQVFVTLPIFLIIYRIVTTLRPIKAIILFNFWDLSKVPLTEIFSNFTSTG", "aa_hexdigest": "2f220ddcb11b846e54f198a5e5a794ec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16096.09469999999, "isoelectric_point": 9.992873573303225 }, "id": "GIHJHMOALN_1430", "locus": "GCA009939745_01430" }, { "type": "cds", "sequence": "contig_1", "start": 807406, "stop": 807555, "strand": "-", "frame": 1, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "COG:COG0594", "COG:J", "EC:3.1.26.5", "GO:0000049", "GO:0001682", "GO:0004526", "GO:0008033", "GO:0030677", "GO:0042781", "SO:0001217", "UniParc:UPI0001DD64AE", "UniRef:UniRef100_P75111", "UniRef:UniRef50_P47703", "UniRef:UniRef90_P75111" ], "nt": "ATGCGGGAACAGTTTTGCAATTTAAACGCTGTTGATGTTTTGGTTATTATTAATCAAGGCTTCCTTGAATTAACATTTAAAGAAAAGCAAACGATCTTTTTAAACCTTTGCCAAAAGCTCCAAGAACTCGATGCCCCTAAACCTAAATAA", "aa": "MREQFCNLNAVDVLVIINQGFLELTFKEKQTIFLNLCQKLQELDAPKPK", "aa_hexdigest": "f7b14340e2ac70ad9eba8d9433301b42", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001DD64AE", "uniref100_id": "UniRef100_P75111", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001DD64AE", "UniRef:UniRef100_P75111" ] }, "ips": { "uniref100_id": "UniRef100_P75111", "uniref90_id": "UniRef90_P75111" }, "psc": { "uniref90_id": "UniRef90_P75111", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_P47703", "cog_id": "COG0594", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0001682", "GO:0004526", "GO:0008033", "GO:0030677", "GO:0042781" ] }, "pscc": { "uniref50_id": "UniRef50_P47703", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47703" ], "product": "Ribonuclease P protein component" }, "genes": [ "rnpA" ], "id": "GIHJHMOALN_1431", "locus": "GCA009939745_01431" }, { "type": "cds", "sequence": "contig_1", "start": 807752, "stop": 807898, "strand": "-", "frame": 3, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "KEGG:K02914", "RefSeq:WP_010875039.1", "SO:0001217", "UniParc:UPI0000133FBC", "UniRef:UniRef100_P78006", "UniRef:UniRef50_Q7X5L3", "UniRef:UniRef90_P78006" ], "nt": "ATGAAACGTACATATCAACCAAGTAAATTAAAGCGAGCAAAAACCCATGGCTTTTTAGCTCGCATGGCCACTGCTTCTGGTCGTAAAGTATTGAAGTTAAGAAGAAAAAAACAACGCGCCCAACTCACCGTATCCAGTGAGCGCTAA", "aa": "MKRTYQPSKLKRAKTHGFLARMATASGRKVLKLRRKKQRAQLTVSSER", "aa_hexdigest": "077659cf990bc319d68efbe827197fe7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FBC", "ncbi_nrp_id": "WP_010875039.1", "uniref100_id": "UniRef100_P78006", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FBC", "RefSeq:WP_010875039.1", "UniRef:UniRef100_P78006" ] }, "ips": { "uniref100_id": "UniRef100_P78006", "uniref90_id": "UniRef90_P78006" }, "psc": { "uniref90_id": "UniRef90_P78006", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_Q7X5L3", "cog_id": "COG0230", "cog_category": "J", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q7X5L3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7X5L3" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "GIHJHMOALN_1432", "locus": "GCA009939745_01432" }, { "type": "cds", "sequence": "contig_1", "start": 807921, "stop": 808505, "strand": "-", "frame": 2, "gene": "lolD", "product": "Putative ABC transporter ATP-binding MG467-like protein", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75110", "UniRef:UniRef90_P75110" ], "nt": "GTGATTATTTTAGGTCCATCAGGTTCGGGGAAAACTACCCTTTTAAACCTAATAGGCGGTTATGACTCAATTTCTTTGGGCAGCTGTGTGGTGGCCAATTGTCCCTTGGAAAAATGTACCACCGAACAGCTCTTAACTTACCGCAAGTTAAACCTGGGTTATGTGTACCAACGCTATAACCTCATAGAACTCTTAAGTGCGTATGACAACATTGCCATCTCGCAAAACCTAATTCCTAAAGAACAACGCCACTTAGACATCGAGGAGTTAGCGGCTAAACTCGACATTAAGGAAATCTTGTACAAGTTTCCGTACGAGATGTCGGGGGGTCAAAAGCAACGGGTGGCGATTGCCCGTGCCATTATTAAAGAACCGCGCTTGTTGCTCTGTGACGAACCTACGGGGGCTTTAGACAGTAATTCAGCCGAAAACATTATTGCCTTATTACAAGCAATCAATAAGGAATATAAGCAAACTATTCTAATGGTGACCCACGATGAAACGTTAACGCGGATCGCTAACAGGATCATTAAGATCTCCGATGGTAAGATAGTGTCTAACGAATTGGTGCGTCCTTTATCTTAG", "aa": "MIILGPSGSGKTTLLNLIGGYDSISLGSCVVANCPLEKCTTEQLLTYRKLNLGYVYQRYNLIELLSAYDNIAISQNLIPKEQRHLDIEELAAKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPRLLLCDEPTGALDSNSAENIIALLQAINKEYKQTILMVTHDETLTRIANRIIKISDGKIVSNELVRPLS", "aa_hexdigest": "99686f010059a8b4df434a739a78c711", "start_type": "GTG", "rbs_motif": null, "genes": [ "lolD" ], "truncated": "3-prime", "pseudogene": { "start": 807921, "stop": 808694, "observations": { "mutation": [ 808589, 808670 ], "stop": [ 808589, 808670 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75110", "query_cov": 1.0, "subject_cov": 0.5722713864306784, "identity": 0.995, "score": 377.0, "evalue": 3.59e-130, "gene_start": 1, "gene_end": 194, "reference_start": 146, "reference_end": 339, "reference_sequence": "MKDTEKKTEKELVWDECVDILENVKANSFKATLEYYLASDKKTKKAKPAKVKKVKEPKAKAVKPEQVKPTKTTKAPKPKKPKKQGGLISQWKEKLEAPYRTPYVEQKQGMVISIDKMWKHVHGEDSKEQIAILSDVSLEIGYGEIVIILGPSGSGKTTLLNLIGGYDSISLGSCVVANCPLEKCTTEQLLTYRKLNLGYVYQRYNLIELLSAYDNIAISQNLIPKEQRHLDIEELAAKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPRLLLCDEPTGALDSNSAENIIALLQAINKEYKQTILMVTHDETLTRIANRIIKISDGKIVSNELVRPLS" }, "paralog": false, "identity": 0.9922178988326849, "score": 493.0, "evalue": 3.9e-178, "description": "Internal stop codon at 808589, 808670. Nonsense mutation around 808589, 808670.", "psc": { "uniref90_id": "UniRef90_P75110", "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG467 homolog", "uniref50_id": "UniRef50_P75110", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75110", "UniRef:UniRef50_P75110", "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75110", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75110" ], "product": "Putative ABC transporter ATP-binding protein MG467 homolog" } }, "id": "GIHJHMOALN_1433", "locus": "GCA009939745_01433" }, { "type": "cds", "sequence": "contig_1", "start": 808668, "stop": 808940, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATACAGAAAAGAAAACCGAGAAAGAACTTGTCTGGGACGAGTGTGTTGATATATTAGAAAACGTTAAGGCCAACAGCTTTAAGGCCACCTTTGAGTATTATTTAGCATCAGACAAGAAAACAAAGAAGGCCAAACCCGCTAAGGTGAAAAAAGTTAAGGAACCGAAGGCGAAAGCAGTTAAACCGGAACAAGTTAAACCCACGAAGACCACCAAGGCACCTAAACCCAAAAAACCGAAAAAGCAGGGTGGGTTGATCTCCCAGTGA", "aa": "MKDTEKKTEKELVWDECVDILENVKANSFKATFEYYLASDKKTKKAKPAKVKKVKEPKAKAVKPEQVKPTKTTKAPKPKKPKKQGGLISQ", "aa_hexdigest": "8579796c9bb4ac0319067d9d10f6f670", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10201.946299999992, "isoelectric_point": 9.882503700256347 }, "id": "GIHJHMOALN_1434", "locus": "GCA009939745_01434" }, { "type": "cds", "sequence": "contig_1", "start": 808981, "stop": 809415, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCCAGCTCTCAAATTAGATGCCGCTGGTGCTAGTGCTCAAATTATCAGGAATAATGCTGAGGTGTTGTTTAATACCGTGAACCAAGTTGATGGGTTCTTGTTGGGCACCATTATTCCCTTTATCTTTATTACCTGTGTGGTCTTGGGGATCTCGATGTTAGAGGAAATGAAACGGATCTTTATTTCCTTAAAATCGATTGGTTACAAAGATAGTCAAAACTTAGTTAGTCTCTTGTGCTTCTTTATTCCCGCTTTTGTACTTTCGTTATTAATCTCGATTGCTATCTTAGCTGGGTTACTAGTAGGAGTACAAGCCCTAGTCTTTGGGGTTGCCCAGGTCTTTTTAACCAACGTGTTTGAATTCTTACCTTACATGGTGGGGATAGTCCTGTTTGGTGCTACCATCTTTGTAATAGGTTCTTACTTCTGA", "aa": "MVPALKLDAAGASAQIIRNNAEVLFNTVNQVDGFLLGTIIPFIFITCVVLGISMLEEMKRIFISLKSIGYKDSQNLVSLLCFFIPAFVLSLLISIAILAGLLVGVQALVFGVAQVFLTNVFEFLPYMVGIVLFGATIFVIGSYF", "aa_hexdigest": "afc3bf22dd50d791de4defe46e49f6af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15601.642999999982, "isoelectric_point": 5.095865821838378 }, "id": "GIHJHMOALN_1435", "locus": "GCA009939745_01435" }, { "type": "cds", "sequence": "contig_1", "start": 809470, "stop": 811185, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCCCTTGGGTAAATTCCGGGTGTCCTTGATTATTTCCCGGTTATCGCGGTTATTTACCTATGTGGGGTTAAGTTCGATTGCCCTGCTTTTAATTGGGATTGCCGGTACCATCCCGCAAAAGTTTGGGGCAGCGCAGTCCAACACCGTTTTAAACCGCAACTTTAACTATAGGTTGAACTTGCAAACCCCCACAGAACAATCCGGCTGGTATGCAATCCAACCCTACTCCCGGTTTGGTCAAACTGATGATAGTTTGGGGATTAAGGCCTTGTATAAAGATAAGGGTGATCAAATTCAGCAACAGCAACAGCAGCAACAACAGCAAGGCAACGACAAAGAACACCCATACAACCTCAAAGAGTTAAAGATTAGTGATAGGGGCGGAAACCCAATCAAGCATAATGGTAAGGAAATCGAACTCGGTAACCTCCTTCTCCCAAGTTTTGGTGGTGCCCAACAATTAAACACTGACGAAAACTTCTTCCGTCATGCTTCGTTGTCGAAGTGGCTCATTGACTTCCCAATTCGCGTTGGTGGTGCCAACATTAACCCGTGGGAAATTGTCGAGAAGTCCATTCCTAAACAAATTACCCAATTGTTATCGGCTTCCAGTGACCAGTTTTTAATTGCGGTTCTTACTGATGACTACTTTAATAACCTCAACAATAATGGTTTCTTAACCCGCAACCCTCGGACTAACTTTATTCAGTTGGATGCCGCTAGGGTATTAACCCAGATTAACGTCTTCAATCCTGGTGGAGTTAAATTTAACGAACAATTCCTTAAATTCCTTACTAAGGTCTATGGTGATCCGGAGTTGTCCTACCAAGACTCGAAGTTAACGTACGGGATTGTCCCGGTTGATCCACAAATAGAGGAGACATACACCTATGTTCAAGGACCATTTGGTTTTAAGGAAACCGAGTTGAATCCTGATTCCCCTTACACCTTAACGGGAATTAGTCCCGATTCCAAGTTTGTCAACCTCACTGACAGCGGTGGTAACTCCTTGAGGAGCTTAATTAGCTCTGACAGTGAGATGAATGTCATTGTCAACGCTGGCTTTCAGTACGCTAATAACACCAAGATCGGGGACTTCATCTTTATTCAACCCAAGAACACTGCGACCCGCTATTCAGAGAAGTTCTTAAAGAGCCCACCTAAAACACCAACCGTGAAGTTCCGGGTTGTCGGGGTATCTACCGATGCCTTTGGCCAAGAGTTGTATATTAACCAAAACATTGCCAACCGCTTGTTAAAGCTCAATGGCTTTGATGGCCGCGGGGTCATTAAGGATGTGGTGAAGGACGGCCAATCCACCGATGATAGCGGTGGTACTTCGTCTGGCGGTGGCTCTTGTGGGGGTGGAAGTACCTCTTCTACCACCAAAGACAAGTACAAGATTGAGTACGTCAAGCCAACTGGTTATGTTCCCTTTAACGGGGTCTTCTCCAAGGAACTCAACCCTTCTTTGGTAAGTAAAGCGCTAGTACTTAACTCCAACATTGGTGTCTGGGGTAACTTTACGGATTTTGGTAATAACTTTACTAACCTCATTAAGGGTAAGGAAAACAAGATTATTACCTCAATCTTACCAAGTGATCCGGATATCTTAAAGCAGTTGGCTAAGGAAAAGGGTGAGAATGGTGTGGATAGCATGACCTATGAAAACCTCAGAAAAAAGGTTATCGAAAAATATACGAGCGAATGA", "aa": "MTPLGKFRVSLIISRLSRLFTYVGLSSIALLLIGIAGTIPQKFGAAQSNTVLNRNFNYRLNLQTPTEQSGWYAIQPYSRFGQTDDSLGIKALYKDKGDQIQQQQQQQQQQGNDKEHPYNLKELKISDRGGNPIKHNGKEIELGNLLLPSFGGAQQLNTDENFFRHASLSKWLIDFPIRVGGANINPWEIVEKSIPKQITQLLSASSDQFLIAVLTDDYFNNLNNNGFLTRNPRTNFIQLDAARVLTQINVFNPGGVKFNEQFLKFLTKVYGDPELSYQDSKLTYGIVPVDPQIEETYTYVQGPFGFKETELNPDSPYTLTGISPDSKFVNLTDSGGNSLRSLISSDSEMNVIVNAGFQYANNTKIGDFIFIQPKNTATRYSEKFLKSPPKTPTVKFRVVGVSTDAFGQELYINQNIANRLLKLNGFDGRGVIKDVVKDGQSTDDSGGTSSGGGSCGGGSTSSTTKDKYKIEYVKPTGYVPFNGVFSKELNPSLVSKALVLNSNIGVWGNFTDFGNNFTNLIKGKENKIITSILPSDPDILKQLAKEKGENGVDSMTYENLRKKVIEKYTSE", "aa_hexdigest": "9ec195fba58d130ae6430f68f12df992", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 63283.47870000016, "isoelectric_point": 8.779836463928223 }, "id": "GIHJHMOALN_1436", "locus": "GCA009939745_01436" }, { "type": "cds", "sequence": "contig_1", "start": 811210, "stop": 811335, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAAGACCGAGAACTTGGTTTCATTACCCTGGTCAATCAGGACATTAACCGGTTTACTAAAGAGCTGTTTAAAGGCTATTCAGCTAATAAACTGGAAGATCACAAAGATCAACAGCATTGA", "aa": "MQEDRELGFITLVNQDINRFTKELFKGYSANKLEDHKDQQH", "aa_hexdigest": "d53d7337194de875612b233604a11f18", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4937.417500000001, "isoelectric_point": 5.473106193542479 }, "id": "GIHJHMOALN_1437", "locus": "GCA009939745_01437" }, { "type": "cds", "sequence": "contig_1", "start": 811294, "stop": 811944, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "TTGTCCACAAACTCACCGCTGCCCAACTCCCAGGATGAAGGCTTAATCTTTCTCAATGAGCAGGGATACCGCAGTGTCTTGTTTGCTGTGCCTGCTGCTAGTGAGGAGAACTACTACGCCTTTAAGTCGGATGACATTAAAGCGAAGTTCCCTGGCCAAGATCCGATCCAGGTGGTGGCCTCTAAGTTAAAGGGCTATCTCAACGTCCCTGACAGTGATCTCGCCTTTAACGTTAAGGACATCTCCAAGTTCCAGTACTTAACTACAGCCCGGAACTATTTCCCGGACTTAGTGCAGAACTATTTAGCCATTGCCAGTGTGGTCATTGCGGCCTTCTTATCCATCTTGGCCTTATACCTCACCATTCTCTTAATTAAGTCTTTTATTAAGAAGAACCAAACGGAGTTCTCAATTATCCGCGCTGGTGGGTTCTCCACAGCCAAGTTTATTGCCGGCATGAGTGTGTTTGCCGGCATAGTAGCCTTGGCCTCATCCTTCTTTGGGGTCTTATTTGCCTTCCTTTTAGAAAGACAAGTTAAGGGGATTATCTCCAGGTATTGGTTTATAGCCCTACCGGCCAACAGCTTTAACTGGATTTCCTTCTTTGGATCAATGCTGTTGATCTTTGTGATCTTCCAGTTTATTAGCTGA", "aa": "MSTNSPLPNSQDEGLIFLNEQGYRSVLFAVPAASEENYYAFKSDDIKAKFPGQDPIQVVASKLKGYLNVPDSDLAFNVKDISKFQYLTTARNYFPDLVQNYLAIASVVIAAFLSILALYLTILLIKSFIKKNQTEFSIIRAGGFSTAKFIAGMSVFAGIVALASSFFGVLFAFLLERQVKGIISRYWFIALPANSFNWISFFGSMLLIFVIFQFIS", "aa_hexdigest": "658120ad7211e625058e72ed5c370a57", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 109, "aa_cov": 0.5046296296296297, "hmm_cov": 0.981651376146789, "evalue": 5.607234042525729e-12, "score": 41.29035568237305, "start": 108, "stop": 216 } ], "seq_stats": { "molecular_weight": 24064.715899999992, "isoelectric_point": 9.023333549499512 }, "id": "GIHJHMOALN_1438", "locus": "GCA009939745_01438" }, { "type": "cds", "sequence": "contig_1", "start": 812080, "stop": 812925, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGAAGGTCACCGCGTTATTAGAAAAATACCGCGATGCCTTCTTAAAGGCGAAAGCCTTAAAGGAATTGGAGTTTTCCCGGCAAAACTTAGCGATTGCTACCAATGTTAGCTCCGAAGAATCCGCTTCCTTCTTGGTCTCCAATAAGGATTCCCAGAAGTACAACGACCTTTCTATTATTGAAGGGATTGACTTTAAGTCTTGGCTTGCCAAGGAAAAAAGCAATCCTTTAGACATGGTATATGGGGGCAAGAGTAACAGTGAAGGCTTCTTGGAAAAGGTTGAGTATGAGTTTAAGCCTACTCAAACAGATGAAAAGAAAAAAGCAGCAGCCAAAACTACGCAAGGCACAACGGACTCTTTAACCCAGTTAGCAGATGCATCTTCTTCCTCCAGCTCCTCTTCAACTGGTGATACTAAGAGTACTTCTACCAAATTCCAAATCTACCCCAAACTAGCTAACATCTTAGCCCAAGCCCAACTCCCCGAGGCTTCCAGTATTCCTGATACCTTAACCAATGCCATTAAGCAGTGGTCTACATTAGATAAAAAAGGCTTTGAGGCTTTAGATGATACAGGTAAGAGTAAAGCCGCTAACAACTATGTTGCTTTATTGAGTTACTTTACCCCGGAGTTTCAAGATCCGAATGAACTGGTAGTAACTAACCGCCAAAAGTTAGATATCCCGATTATCTTTAAAAACGGGGTTAACCCGTTAACACTACCCACCGACCAACAGTCCTTGGTAGTCCAAACCCCGGAAGCCCACGGTGCGGTGGTATCCCAGCAATGGTTATTTAAACACGACAAGGAAGTATTACCTTTAGAAGGGGAATACTCCTGA", "aa": "MKKVTALLEKYRDAFLKAKALKELEFSRQNLAIATNVSSEESASFLVSNKDSQKYNDLSIIEGIDFKSWLAKEKSNPLDMVYGGKSNSEGFLEKVEYEFKPTQTDEKKKAAAKTTQGTTDSLTQLADASSSSSSSSTGDTKSTSTKFQIYPKLANILAQAQLPEASSIPDTLTNAIKQWSTLDKKGFEALDDTGKSKAANNYVALLSYFTPEFQDPNELVVTNRQKLDIPIIFKNGVNPLTLPTDQQSLVVQTPEAHGAVVSQQWLFKHDKEVLPLEGEYS", "aa_hexdigest": "f56050dcc3cad352fdd52eb05347d396", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31021.418700000017, "isoelectric_point": 5.449574851989746 }, "id": "GIHJHMOALN_1439", "locus": "GCA009939745_01439" }, { "type": "cds", "sequence": "contig_1", "start": 812950, "stop": 813171, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGCACTTCGCTCTTCTGTTTCTTTCAAGCCGCAATTACAACAAACGAACGTTGCGTTGGCTCAGCAACAACAAGATAAGCAGGAAAGTAGTGCAGATGACGGTGTAAAAGATCCAACTTTTAGTGATGTCCAAACTGAATTCGACAAAATTGGCACCGAAAACCACACTCCGCAAAAGAATTTAAATAACGTTTATGCTGCCCTTTTACACCAATGA", "aa": "MIALRSSVSFKPQLQQTNVALAQQQQDKQESSADDGVKDPTFSDVQTEFDKIGTENHTPQKNLNNVYAALLHQ", "aa_hexdigest": "056c214fb13dbbed568e977ab32818e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8143.847, "isoelectric_point": 5.034650230407714 }, "id": "GIHJHMOALN_1440", "locus": "GCA009939745_01440" }, { "type": "cds", "sequence": "contig_1", "start": 813418, "stop": 814590, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCGTTTTTTAAACAGATCTTTAAGTCACTCAAAAAGTTCTTCTTTTTACTTTTTGGGATTATTTTCGTCCTTTTCAGCATTATCTTCCTGGAAACGTCGATTCTTCAGTTAAGTAATAACCTTGTTAATACTTATACTGCCCTTGTTCAAAAAACGAATTCTTCTGACATAGTAGCACCGGCTATCTTTAAAGAAAGTAGTCCGGTGTATAAAACGGAACTTAAAGAAGAAAAAAGACATTTTTCCAAAATCAAGTTAACCGAAAAGAAGATTAACTTTATCTGGCCCTACCAAGAATCTGATTTTGGGAGTGATTCTGAAAAGAAAGATTCAACCACCAAATCATCAGATAATAACCCACGAAAAGGTGATGTTAACGATAAAGACAAACTCTTTTTAGCTCGTAAAAGAGGCATTTTGAAAGCTTACGGGGAAGCCAACATTGCCGAAAAACGGATCTATAAGGGTTTAGCTGTTTCCTTTCAAAATACCTATTCTTTTACTGGAACCGATCAAGAAAGTAATAACTTAAACCAAAATACGGTTTCCGATCCCCAAAACCTGATTTATGACAAAGAAGGTAACCTTTTAGGTTACTTTGTCGATGGTTTAATTCTGGATGGGATTCCCCTGCGCGCTGGGATTGCTCGCTTTCCTGGTGATAAAGGGAAGGGTGAGGATAAGAAAACTACTAAAAAAGAATCAGAAATCAAGCAAGCGAGTAGTGCCACAACAGTTTTACAACCGTTAGCAGCTCAGGCCAAGATGACTGATGCTAAAGAAACCACAAATAACGAGGAGCCCAAAAAAGATAGTAATGTAGAAGAACAATACACTACCAATAATAAAGACAAAGTTTGGTTTAAAAGTGATGAAACTCAAGCTGGTAGTAGCAGTGGAGAAAGCGAAACCAGTAAACTAAGTACCTCTTATCTATTTACCGGAGGGCAAGAAGCTGCTAACTGGTTTCCCAATCTGTACGCTAATGTACCTATTGTGCTCCCGATTAGTCCGGGTTCCCAGTTTTGGTTAGAGACTAATCCCTTTAAAGAAATAATAGAGGTCTTCCAAAAAGAAAAAGAGGAAAAAGAAAAACAATCTTTCTCCTTAACTTTTACTTTAGATACAAGTAAGTTATCTCATTTAGATAAGGAAGAATTTGACTGA", "aa": "MFSFFKQIFKSLKKFFFLLFGIIFVLFSIIFLETSILQLSNNLVNTYTALVQKTNSSDIVAPAIFKESSPVYKTELKEEKRHFSKIKLTEKKINFIWPYQESDFGSDSEKKDSTTKSSDNNPRKGDVNDKDKLFLARKRGILKAYGEANIAEKRIYKGLAVSFQNTYSFTGTDQESNNLNQNTVSDPQNLIYDKEGNLLGYFVDGLILDGIPLRAGIARFPGDKGKGEDKKTTKKESEIKQASSATTVLQPLAAQAKMTDAKETTNNEEPKKDSNVEEQYTTNNKDKVWFKSDETQAGSSSGESETSKLSTSYLFTGGQEAANWFPNLYANVPIVLPISPGSQFWLETNPFKEIIEVFQKEKEEKEKQSFSLTFTLDTSKLSHLDKEEFD", "aa_hexdigest": "0f29f60cd8cf78a70cbef571f826d49b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 44162.08070000007, "isoelectric_point": 6.015975379943847 }, "id": "GIHJHMOALN_1441", "locus": "GCA009939745_01441" }, { "type": "cds", "sequence": "contig_1", "start": 814602, "stop": 815267, "strand": "-", "frame": 2, "gene": "lolD", "product": "Putative ABC transporter ATP-binding MG468-1-like protein", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_Q50316", "UniRef:UniRef90_Q50316" ], "nt": "GTGACCAATGGGATCACAAACCAGTTAATCTGTGATCACATTGATCTCAAAATTGCCCCTGGGGAGTTTGTGGTTATTTTGGGTAAATCTGGTTCTGGTAAGACTAGTTTACTCTCTTTAATCTCCGCCCTAGACCGACCTACTAGCGGGGTGAGTTTTGTGTGTGGTAGGAGTACTATTTGTTGTAATGATGCCCAATTAACATCACTACGGAATAAAAACGTAGGTTATATATTCCAACAGTATGGCCTTTTAAGGGATCTGAACGTTGATGACAACATTAAACTGGCAGTTCCCTTTAAAAAGCGCCATAACAACAATTTGGAAGAGCTGTTAGAGCGGTTGGAGCTCAAAGAGCACCGTAATAAAAAGATTACCAAGCTCTCTGGGGGACAACAACAACGGGTAGCAATTGCGCGGGCACTAATCAAAGAGCCGCGTATTTTATTTGGTGATGAGCCAACTGGTGCTGTTAACGTGGATATTTCCAAAAAGATCCTCCAGTTCTTTGTGGAGTATAACCGCGATAAGGGCACTACTATAGTATTAGTTACCCACAATGAAAAGATTGTGGAGCTAGCTAAACGGGTCATTAAGATCCACGATGGCAAGATTGTGGCTGATTATCTTAACCAAAGACCCAAGACTATTAACGAAATTAACTGA", "aa": "MTNGITNQLICDHIDLKIAPGEFVVILGKSGSGKTSLLSLISALDRPTSGVSFVCGRSTICCNDAQLTSLRNKNVGYIFQQYGLLRDLNVDDNIKLAVPFKKRHNNNLEELLERLELKEHRNKKITKLSGGQQQRVAIARALIKEPRILFGDEPTGAVNVDISKKILQFFVEYNRDKGTTIVLVTHNEKIVELAKRVIKIHDGKIVADYLNQRPKTINEIN", "aa_hexdigest": "9b2835fec29bde0f45f83c1213c1044d", "start_type": "GTG", "rbs_motif": null, "genes": [ "lolD" ], "truncated": "3-prime", "pseudogene": { "start": 814602, "stop": 815450, "observations": { "mutation": [ 815402 ], "stop": [ 815402 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q50316", "query_cov": 1.0, "subject_cov": 0.775438596491228, "identity": 0.995, "score": 428.0, "evalue": 1.3e-150, "gene_start": 1, "gene_end": 221, "reference_start": 63, "reference_end": 283, "reference_sequence": "MSLNAKNKRSLDYCLQWPDFCQSKKASKLIVKLNKKHPKRRXHYKDPEAKHYDVLFKGVCKAVTNGITNQLICDHIDLKIAPGEFVVILGKSGSGKTSLLSLISALDRPTSGVSFVCGRSTICCNDAQLTSLRNKNVGYIFQQYGLLRDLNVDDNIKLAVPFKKRHNNNLEELLERLELKEHRNKKITKLSGGQQQRVAIARALIKEPRILFGDEPTGAVNVDISKKILQFFVEYNRDKGTTIVLVTHNEKIVELAKRVIKIHDGKIVADYLNQRPKTINEINWV" }, "paralog": false, "identity": 0.9929328621908127, "score": 555.0, "evalue": 4.84e-203, "description": "Internal stop codon at 815402. Nonsense mutation around 815402.", "psc": { "uniref90_id": "UniRef90_Q50316", "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG468.1 homolog", "uniref50_id": "UniRef50_Q50316", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q50316", "UniRef:UniRef50_Q50316", "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q50316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50316" ], "product": "Putative ABC transporter ATP-binding protein MG468.1 homolog" } }, "id": "GIHJHMOALN_1442", "locus": "GCA009939745_01442" }, { "type": "cds", "sequence": "contig_1", "start": 815519, "stop": 816838, "strand": "-", "frame": 3, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "GO:0003688", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006270", "GO:0006275", "GO:0016887", "KEGG:K02313", "RefSeq:WP_159202201.1", "SO:0001217", "UniParc:UPI00132FBBC1", "UniRef:UniRef100_UPI00132FBBC1", "UniRef:UniRef50_P35888", "UniRef:UniRef90_Q59549" ], "nt": "ATGGAACAATTTAGTGCTTTTAAACTTCTTTTAAAAAAGCAATACGAAACTACTTTAGGTTTTTATGACAAGTACATTAAGAATCTTAAACGGTTTGCTTTAAAAAATAATGTCCTTTTTGTGATTGTGGACAATGAGTTTGCCCGTGAAACCCTCAATAGCAACCCTGATATTGTCGGGTTAGCTAGCAAACTGTACGATGACATTAGAGCAGTTCGGTTTGTCAACGAACAGGACTTCTTTATCAATTTAGCCAAACTAGAGGAAGATAACCGCGAAACGCTTTACCAAAGTTCGGGGCTAAGCAAAAACTTTACCTTTAAAAACTTTGTGGTTGGTGATGGCAACCGCCGGGTTTATGAAGCGGGTGTACGGATTAGCGAAACTCAGGACGCTGATTTCTCCCCCTTATTTATCTATGGGGAGACTGGTCTGGGTAAAACCCACTTACTCCAAGCGATTGGGAATGATAAGTTCTTCCACTTTCCCAACGCTAAGGTAAAGTATGTGGTATCGAGTGACTTTGCCCAGGAGGTTGTCAATGCCTTTTACCAAAAGGACAAGGATCAAGGCATTGAAAAGCTCAAGGCTGCTTACGAGTCATTGGATTTACTCTTAATTGACGATACTCAGATCTTCGGGAAAAAGGAAAAAACCTTAGAGATCCTCTTTTCCATCTTTAATAACTTAGTAAGTAAGGGCAAGCAAATTGTGTTTGTGTCCGATAAAACCCCCGATGAGTTGGCCAACATCGATCCACGGATGGTATCACGCTTTAAATCGGGACTCTTGTTAAAGATTGAAAAACACAATTTGAGCTCCCTGTGTGAAATTTTGGCCGTTAAGCTCAAAGAAAAGGATCCCAACATCCAAATTACCAATGAAGCACGCGAAAAGGCAGCGCAAATATCTGGTAACGATGTTCGTTCTTTAAACGGGATTGCGACTAAGTTGCTGTTTTATGTCAAAACAACTAAGCAAAACCTGATTAACAACGACAATCTAAAGGAAATCCTGTTTGAGGAATTTGAGAAGTTCCACAAAAAGAGCTTTGATCCTTATTTATTAATAGAAAACGTTTGCCGCCGCTTTAATGTACCGGTGGACAGTGTTTTGTCTGAGAACCGTAAAGCAGAGTTAGTTCGTGTTCGTGACATTTGTAACTATCTTTTAAGGGAAAAGTACAAGATGCAGTTCCAACAAATAGGCAAAATTTTTAAAAGGAACCACTCTACAGTGGTGGCTGCAGTTAAAAGGGTTGCTAAAATGATTGAGAAGGACGACTCCCTCCAAGACATTATCACTTCCTTGGTAATTTAG", "aa": "MEQFSAFKLLLKKQYETTLGFYDKYIKNLKRFALKNNVLFVIVDNEFARETLNSNPDIVGLASKLYDDIRAVRFVNEQDFFINLAKLEEDNRETLYQSSGLSKNFTFKNFVVGDGNRRVYEAGVRISETQDADFSPLFIYGETGLGKTHLLQAIGNDKFFHFPNAKVKYVVSSDFAQEVVNAFYQKDKDQGIEKLKAAYESLDLLLIDDTQIFGKKEKTLEILFSIFNNLVSKGKQIVFVSDKTPDELANIDPRMVSRFKSGLLLKIEKHNLSSLCEILAVKLKEKDPNIQITNEAREKAAQISGNDVRSLNGIATKLLFYVKTTKQNLINNDNLKEILFEEFEKFHKKSFDPYLLIENVCRRFNVPVDSVLSENRKAELVRVRDICNYLLREKYKMQFQQIGKIFKRNHSTVVAAVKRVAKMIEKDDSLQDIITSLVI", "aa_hexdigest": "3902ad5d45917eea0dc42a34186f88ed", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00132FBBC1", "ncbi_nrp_id": "WP_159202201.1", "uniref100_id": "UniRef100_UPI00132FBBC1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00132FBBC1", "RefSeq:WP_159202201.1", "UniRef:UniRef100_UPI00132FBBC1" ] }, "ips": { "uniref100_id": "UniRef100_UPI00132FBBC1", "uniref90_id": "UniRef90_Q59549" }, "psc": { "uniref90_id": "UniRef90_Q59549", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_P35888", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "go_ids": [ "GO:0005737", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0003688", "GO:0006270", "GO:0006275" ] }, "pscc": { "uniref50_id": "UniRef50_P35888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35888" ], "product": "Chromosomal replication initiator protein DnaA" }, "genes": [ "dnaA" ], "id": "GIHJHMOALN_1443", "locus": "GCA009939745_01443" }, { "type": "cds", "sequence": "contig_1", "start": 816838, "stop": 817590, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MPN_687", "db_xrefs": [ "RefSeq:WP_010875044.1", "SO:0001217", "UniParc:UPI000013AFF4", "UniRef:UniRef100_Q50315", "UniRef:UniRef50_Q50315", "UniRef:UniRef90_Q50315" ], "nt": "ATGGCCATCAGTAAAAAGAAACGCTTCTTCTTTGACCTAGCTCAGGATGAAGATGATGCAGAAACTGTTCAAGAAGTTAAAAAGGTAGAACAACAATTAAAACTAGAACCGGTTGTCCAACCCCAACATGATCTAACTAACCAAACTAAAGCAAATCAATCTAGTCAAGACAGAAAGTTCTTTTCTAAGGACATGCCCCAGTTTGATTTTGGACCATTACTCAAGTTTGGCGATGAGTTTGTGAAATCCTTTAACCAGTTTCCCAAACAAGAACCACAAACTTCAACCCAACCAGTTAATGTGCAACCGCAATCAGAACCAACTAACTTTAATAATCAGGTTCCAACCCAACCAGTTCACCAAACTGCAGAAGTGCACTTAAATGAGTTTCAACAACCTACAACCACTAACTTTAATCAACAACCAGTAGCTACATCTAATATTCAGGTGGAGGCTACGCAACCAATTGTGGAACCTGTTCCCCAACCAGAACCACAACCAGCGGTTGAACAGCCACAAGTTAAGCAGACTACTCGCCCATCTAATAAATTGCAGGAAGAAGAAAATCTACCACCACCAAAGGCGAAAGTGCCCGGTATTATTCCCTTAGAACGGCAAGAACGCTTAACCACCGGGGTGCACTTTTATACGAGTACCCGTGTTTGGAATAAGGTTAAGCGTTATGCTAAAGCGGTTAACATTCCGATTTCGCGGATCTTAACGATGATTCTTGATCAGGTAATAGAAGAGTAA", "aa": "MAISKKKRFFFDLAQDEDDAETVQEVKKVEQQLKLEPVVQPQHDLTNQTKANQSSQDRKFFSKDMPQFDFGPLLKFGDEFVKSFNQFPKQEPQTSTQPVNVQPQSEPTNFNNQVPTQPVHQTAEVHLNEFQQPTTTNFNQQPVATSNIQVEATQPIVEPVPQPEPQPAVEQPQVKQTTRPSNKLQEEENLPPPKAKVPGIIPLERQERLTTGVHFYTSTRVWNKVKRYAKAVNIPISRILTMILDQVIEE", "aa_hexdigest": "1fa6f86f9aea8ab7acbbe64c6fd4afe0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013AFF4", "ncbi_nrp_id": "WP_010875044.1", "uniref100_id": "UniRef100_Q50315", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013AFF4", "RefSeq:WP_010875044.1", "UniRef:UniRef100_Q50315" ] }, "ips": { "uniref100_id": "UniRef100_Q50315", "uniref90_id": "UniRef90_Q50315" }, "psc": { "uniref90_id": "UniRef90_Q50315", "product": "Uncharacterized protein MPN_687", "uniref50_id": "UniRef50_Q50315" }, "pscc": { "uniref50_id": "UniRef50_Q50315", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50315" ], "product": "Uncharacterized protein MPN_687" }, "genes": [], "id": "GIHJHMOALN_1444", "locus": "GCA009939745_01444" }, { "type": "cds", "sequence": "contig_1", "start": 817577, "stop": 818389, "strand": "-", "frame": 3, "gene": "parA", "product": "ParA family protein MPN_688", "db_xrefs": [ "COG:COG1192", "COG:DN", "KEGG:K03496", "RefSeq:WP_010875045.1", "SO:0001217", "UniParc:UPI0000131320", "UniRef:UniRef100_Q50314", "UniRef:UniRef50_P47706", "UniRef:UniRef90_Q50314" ], "nt": "ATGATTATTTCTTTTGTTAATAACAAAGGTGGCGTTTTAAAAACAACCATGGCCACTAACGTTGCTGGTTCGCTTGTAAAGTTGTGCCCTGAACAACGGAAAGTTATTTTAGACCTTGATGGTCAGGGCAACGTTTCAGCCTCTTTTGGACAAAATCCAGAAAGATTAAATAACACCTTAATTGATATTTTGTTAAAAGTGCCTAAGTTTAACGGCGCCAACAGTTCAATTGAAATTGATGACTGTTTGTTGCCCGTTTATGAAGGACTAGACATTTTGCCTTGTAACTTTGAGTTAAATTTTGCGGACATTGATATTGCTCGCAAAAAGTACAAGGCTTCTGATATTGCGGAAATAGTTAAACAACTCACCCGCAGATATGACTTTGTTTTACTAGACACACCGCCTAACATGGCTACCTTGGTCTCCACAGCCATGAGCCTCTCTGATGTGATTGTGATCCCGTTTGAACCTGATCAATACTCTATGTTGGGTTTAATGCGGATTGTCGAAACGATTGATACTTTTAAGGAAAAGAACCCCAACTTAAAAACCATTTTGGTACCAACCAAGGTCAATATGCGCACCAGATTACACAATGATGTGATTGAGTTGGTGAAATCAAAAGCGCACAAGAACAACGTTGCTTTTTCAGAACACTTTGTATCTTTAACATCTAAGTCATCTGCTGCTGTTGGTTATGAAAAGTTACCTATCTCTTTGGTATCACCAACATCCAACAAGAAGTACCAAACTGAATATTTAGAGATTACTAAAGAAATTCTTAACCTAGTCAACCATGGCCATCAGTAA", "aa": "MIISFVNNKGGVLKTTMATNVAGSLVKLCPEQRKVILDLDGQGNVSASFGQNPERLNNTLIDILLKVPKFNGANSSIEIDDCLLPVYEGLDILPCNFELNFADIDIARKKYKASDIAEIVKQLTRRYDFVLLDTPPNMATLVSTAMSLSDVIVIPFEPDQYSMLGLMRIVETIDTFKEKNPNLKTILVPTKVNMRTRLHNDVIELVKSKAHKNNVAFSEHFVSLTSKSSAAVGYEKLPISLVSPTSNKKYQTEYLEITKEILNLVNHGHQ", "aa_hexdigest": "2a80685578b86c1abf65fb4d027b6b5e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000131320", "ncbi_nrp_id": "WP_010875045.1", "uniref100_id": "UniRef100_Q50314", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000131320", "RefSeq:WP_010875045.1", "UniRef:UniRef100_Q50314" ] }, "ips": { "uniref100_id": "UniRef100_Q50314", "uniref90_id": "UniRef90_Q50314" }, "psc": { "uniref90_id": "UniRef90_Q50314", "gene": "ParA", "product": "ParA family protein MPN_688", "uniref50_id": "UniRef50_P47706", "cog_id": "COG1192", "cog_category": "DN", "kegg_orthology_id": "K03496" }, "pscc": { "uniref50_id": "UniRef50_P47706", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47706" ], "product": "ParA family protein MG470" }, "genes": [ "parA" ], "id": "GIHJHMOALN_1445", "locus": "GCA009939745_01445" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Mycoplasmoides pneumoniae] [strain=16-734] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TGTGCCCAACCGCGAAAAAGAAAGTGGTATATAGGAAAATGGAAAACAAATTTGTTTACCGTTTACTATTAAATACTAATCTTTTATATAGTATAGAGAAACTTTTTCTTTAACATAATATTATCTTAATATTATTTACCTACTAATAGCTTAATATTATTAGTATTTATTTAGTATTATGCTAATACTATGCAGATATTATCTTAATATTATCTATAGTATTAGGCTAATATTATTCTTAATATTTATTAAGGTACTAAAGCATTACCTATAGGTGATATTATGACAATACTAAAGTGGTTAGTATTATTAGGGTATTATTCAAAGTATTCTCCAACACTATTCCCTTAGCTCTAACTTAATATCCCTTTAATACCAAGATGGTTAAACAATGAAGAAAATTAGTTGTTTGTTCTGGCCCTTTACTATATATGTACATAGTACATATATAAGAAAGGCTACCATGACATTATATATAATAGGCATCCAGAAATAAAAGCCAAATTAATCTATAATTAAGTATTTATACTTAATTATAGTGATTTTATTAACAAAATTAGTGACATTTGGCCATCCATAATAAATAAAATCAAATAAACAAAACAAAAAGAAAAAAACTATAAAAAGGGCATGAAAGTTTTGATTAATAAGAATGAGTTGAACAAAATCCTCAAAAAACTCAACAATGTAATCGTATCTAACAATAAGATGAAACCATACCACTCTTATTTATTAATAGAGGCTACAGAAAAGGAAATTAACTTCTATGCTAACAACGAGTACTTTTCTGCTAAATGTACCTTAGCCGAAAACATTGATGTACTTGAAGAAGGTGAAGTAATTGTTAAAGGCAAAATCTTTAGCGAACTCATTAACGGAATTAAAGAAGACATCATTATTATTCAAGAGAAAGATCAAACTCTTTTAGTCAAAACAAAAAAAACAAACATTAACCTTAACACGATTGATAAGAAAGAATTCCCCAGAATCCGTTTCAACCAAAACGTTGATTTGAAGGAATTTGATGAACTTAAAATCCAACACAGCCTTTTAACTAAAGGACTTAAAAAGATTGCCCATGCTGTTTCTACATTTAGAGAATCCACTAGAAAATTCAACGGGGTTAACTTCAACGGTTCCAATGGTAAACAAATCTTTTTAGAGGCATCGGATTCTTATAAGCTCTCTGTTTATGAAATCAAACAAAAAACCGATCCATTTAATTTCATTGTCGAAACTAATCTTTTGAGCTTCATCAATTCTTTTAACCCTGAAGGTGGTGATTTAATCAGTATCTTCTTCCGCAAAGAACACAAGGATGATTTAAGTACCGAATTACTGATTAAGTTAGATAACTTCTTAATTAACTACACCTCAATTAACGAAAGCTTTCCGCGGGTAATGCAGTTGTTTGACTTTGAACCAGAAACCAAAGTAACCATTCAAAAAAACGAACTTAAAGATGCATTACAAAGAATCTTGACGTTAGCTCAAAACGAGCGCTTCTTTCTCTGTGACATGCAAGTAACCAACTCCCACCTCAAAATTAATTCCAACGTTCAAAACATTGGTGCATCCTTAGAAGAAGTTACTTGCCTTAAGTTCGAAGGTCACAAACTCAACATCGCTGTTAATGCGCTTTCCCTCTTGGAACACATTGACTCATTTGATACTGATGAAATTGAGCTTTACTTCCAGGGCAGTAACAAATACTTTTTAATTAGTTCGAACAACGAACCTGAACTTAAAGAAATCCTAGTTCCTTCCAAGTAACTAATGACGCTTTATGACTTACTAGAGCTGCCCCAAACTGCTACACTACAGGAAATCAAAACAGCTTACAAACGCTTAGCTAAACGTTATCACCCTGACATCAATAAACAAGGGGCAGATACCTTTGTCAAGATTAATAACGCCTATGCCGTTTTAAGCGATACCACCCAAAAAGCGGAGTATGACGCTATGCTACGCTTTAGTGAATTTGAAGACCGAGTTAAACGCCTGGACTTCTCGATTAAGTGACACGAACAGTTTATGGAGGAATTACAGTTCCACCACAACTGGGACTTTGACTTTATCCGCAACAGGGAATACACCCAGCCAACCCCTACCAACAATAAGTATTCTTCCTTTTTAGACAAGGATGTATCCTTGGCCTTTTACCAACTTTACTCCAAGGGCAAGTTGGACTTTGACTTGGAAGACACACTTCTTAGACGTCATTCGATCAAGCAAGCCTTCTTAAAGGGCAAGAAGCTTAACGATGTTTTAAAAGAGCAGTACAACTATTTAGGTTGATTAGAGGCCAAGCGCTATTTCAACATTGATGTCGAAATCGAATTAACCCCCAAAGAAGTGCGTGAAGGTGGGGTGGTCAACCTCCCTTTAAAGATCAAAGTCATTTCCAATAACTACCCTGGACAAATGTGGTACGAACTCAACAAGAACTATTCCTTCCGGCTTCTTTGAGACATTAAAAACGGTGAGGTGGCAGAGTTCTTTGGCAAGGGTAATCGGGCCTTGGGTTGACGCGGTGATCTGATTGTGCGAATGCGGATTGTCGACAAAATTAAGAAGCGCTTACGCATCTTTTCCAGTCATTTTGAACAGGACAAAACCAAGTTGTGATTCCTCGTGCCGCAGGACAAACAAGACAATCCTAATAAGTGGGTTTTTGACTATAAAACACACGAGTTTATTGTTTAAAATCGCTTAATTTTTAATGTGGTATAATTGTTTGGATTCGCCATAAATTTTGCTTATGGCTTTTAACTTTCCAACGTGCACATTAAATGGAAGACAATAACAAAACGCAAGCTTACGATTCCAGTAGCATTAAGATTCTTGAAGGCTTAGAGGCCGTGCGTAAGCGCCCCGGCATGTACATCGGTTCCACCGGCGAAGAAGGGTTGCACCACATGATCTGGGAAATCATTGATAACTCAATTGACGAGGCCATGGGTGGCTTTGCGAGTACTGTCAAACTAACCCTTAAAGACAACTTTGTCACCATTGTGGAGGATGATGGCCGGGGCATTCCCGTTGACATCCATCCTAAGACCAACCGCTCCACCGTTGAAACAGTGTTTACGGTACTCCACGCTGGGGGTAAGTTTGACAACGATAGCTATAAGGTGTCAGGGGGACTCCACGGTGTGGGTGCCTCGGTTGTTAATGCTTTAAGTTCCTCCTTTAAGGTATGGGTAGCCAGGGAGCACCAACAGTACTTCCTAGCGTTCCACAACGGTGGGGAAGTGATTGGTGATCTTGTCAACGAAGGTAAGTGTGACAAGGAACACGGTACCAAGGTTGAGTTTGTCCCGGACTTTACCGTGATGGAAAAGAGTGATTACAAACAAACGGTAATTGCTAGTAGGTTGCAACAATTAGCCTTTCTCAACAAAGGGATCCAAATTGACTTTGTTGATGAACGCCGTCAAAATCCGCAAAGCTTTTCTTGGAAGTATGATGGTGGCTTAGTCCAATACATCCACCACCTCAACAACGAAAAGGAACCTTTATTTGAGGACATTATCTTTGGTGAAAAAACCGATACTGTTAAATCAGTTAGCCGTGATGAGAGCTACACAATTAAGGTGGAAGTGGCCTTTCAGTACAACAAGACGTATAACCAATCAATCTTTAGTTTTTGTAACAACATTAATACCACTGAAGGCGGTACCCACGTTGAAGGCTTTCGCAATGCCTTAGTGAAGATCATTAACCGCTTTGCTGTCGAAAACAAGTTCTTAAAGGAAACGGATGAAAAGATTACCCGCGATGACATCTGTGAAGGGTTAACGGCCATTATCTCAATTAAGCACCCCAACCCCCAGTATGAGGGTCAAACCAAAAAGAAATTGGGTAACACCGAAGTGCGTCCTCTAGTTAACAGTATTGTTAGTGAAATCTTTGAGCGCTTTATGTTGGAAAACCCCCAGGAAGCCAACGCCATTATTCGCAAAACGCTGTTAGCGCAAGAAGCACGGCGCCGTAGTCAGGAAGCACGGGAACTGACCCGCAGGAAATCTCCCTTTGACAGTGGTTCACTCCCGGGTAAGTTAGCAGACTGTACCACCCGTGATCCCTCAATTAGTGAACTGTACATTGTCGAGGGAGATAGCGCTGGTGGTACGGCTAAAACGGGGCGGGACCGTTACTTCCAAGCGATCCTACCATTAAGGGGTAAGATCCTCAATGTGGAAAAGTCCCACTTTGAACAAATCTTTAATAATGTGGAAATCTCGGCTTTAGTCATGGCCGTTGGCTGTGGCATTAAACCAGACTTCGAGTTGGAAAAACTGCGGTATAACAAGATCATTATCATGACCGATGCTGATGTCGATGGGGCGCACATCCGTACCCTCCTTTTAACCTTCTTCTTTCGCTTTATGTATCCCTTGGTGGAACAGGGCAACATTTACATTGCCCAACCCCCACTGTATAAGGTCTCTTACTCTAATAAGGACTTATACATGCAAACCGATGTCCAACTAGAGGAGTGGAAGCAACAACACCCTAATCTGAAATACAACTTACAACGCTACAAGGGTTTAGGGGAAATGGATGCCATCCAACTGTGGGAAACCACGATGGATCCCAAGGTGCGTACCTTACTAAAGGTAACCGTTGAAGATGCTTCAATTGCTGATAAAGCCTTCTCCTTGTTAATGGGCGATGAAGTTCCCCCAAGACGGGAATTCATCGAACAAAACGCACGTAACGTAAAGAACATTGATATCTAATGGCAAAGCAACAAGATCAAATTGACAAAATTCGTCAGGAATTAGCACAATCAGCAATTAAAAACATCTCCCTCTCCAGTGAACTAGAACGTTCCTTCATGGAGTATGCGATGTCGGTGATAGTGGCTCGCGCGCTCCCAGATGCGAGGGATGGACTTAAACCTGTGCACCGCCGTGTGCTCTATGGTGCTTACACTGGGGGCATGCACCACGATCGCCCCTTTAAAAAGTCAGCACGGATTGTCGGTGATGTAATGAGTAAGTTCCACCCCCACGGGGACATGGCCATTTACGACACAATGTCGCGGATGGCGCAGGACTTTTCTTTGCGTTACCTCTTAATTGACGGACATGGTAACTTTGGCTCGATTGATGGCGATCGTCCCGCAGCACAACGTTATACGGAAGCACGGTTGTCGAAATTAGCGGGGGAACTGTTACGTGGCATTGACAAGGACACGGTCGACTTTGTCGCTAACTATGATGGGGAAGAACAAGAACCAACGGTTTTACCAGCTGCCTTTCCTAATTTATTAGCTAATGGTTCGAGTGGGATTGCGGTAGGGATGTCCACTTCCATTCCGTCTCACAACTTATCGGAGTTAATCCAGGGCTTAATCCTTTTAATTGACAACCCTGACTGCACCATTAATGATTTATTAGGTGTCATTAAAGGACCGGACTTTCCCACCGGAGCCAACATTATTTACACCAAGGGGATTGAAAGCTACTTTGAAACTGGGAAGGGTAATGTGGTCATTCGTTCTAAAGTCAGCATTGAACAACTACCAACCCGGGCTGCTTTAGTAGTCACGGAAATTCCCTACATGGTCAACAAGACCAGTTTAATTGAAAAGATTGTCGAGTTAGTCAAGGCTGAGGAAATCACCGGGATTGCCGATATCCGTGATGAGTCATCCAGGGAAGGAATTCGCTTGGTGATTGAAGTGAAGCGCGACACCGTTCCGGAAGTCTTATTAAACCAACTGTTCAAATCAACCCGTTTACAGGTGCGGTTCCCCGTGAACATGTTGGCACTGGTTAAGGGTGCACCTAAATTGCTCAACATGAAGCAAGCGTTAACGGTTTACCTCGAGCACCAACTCGATGTTCTGATTCGCAAAACCCAGTTTAACCTCAAGAAGTACCAGGAGCGCTTTCATATCTTAAGTGGCCTTTTAATTGCGGCGTTGAACATTGATGAGGTAATTGCGATCATTAAAAAGTCCGCTAATAACCAGGTAGCAATGGAAGCACTCCACGAACGCTTCGGGTTAGATGAAATCCAAGCACGGGCGGTTCTGGACATGCGTTTACGTAGCTTAAGTGTCTTGGAAGTCAATAAACTACAAACCGAACAACAAGAGTTGAAAGCTTTAATTGAGTTCTGTCAACAGGTGTTAGCTGACAAACAACTGCAACTCAAACTAATTAAGGAGCAGTTAACCAAGATTAACGAACAGTTTGGCGATCCACGCCGTAGTGAAATCCTGTACGGTATTAGTGAAGACATTGATGATGAGGACTTAATTACCCAGGAAAACGTGGTAATCACGATGTCCACTAACGGTTATCTCAAACGGATTGGGGTAGACTCTTATAACCTGCAACACCGTGGTGGGGTGGGTGTTAAGGGTTTAACTACCTACACCGATGACAGTATTAGCCAGCTGTTAGTCTGCTCGACCCACTCGGACCTCTTATTCTTTACGGACAAGGGAAAGGTCTACCGCATCCGGGCCCACCAAATTCCCCCGGGTTTTCGTACTAACAAGGGGATTCCGGCGGTTAACCTGATTAAGATCGACAAGGACGAGAAGATCTGTGCCTTAATTTCGGTGAATGATTACCAAAATGGTTACTTCTTCTTTTGTACCAAGAACGGTACCATTAAGCGCACCAGTCTAAGTGAGTTTGCCAACATCTTGAGTATTGGTAAACGCGCCATCCTCTTTAAGGAAAACGATGTTTTGTTCTCGGTTATTCGCACCAGTGGTCAAGATGACATCTTTATCGGTTCGACCGCGGGGTTTGTCGTGCGTTTCCACGAAGACACCGTCCGTCCGCTGTCCCGCGCTGCCATGGGGGTCTTGGGGATTAATCTCAACCAGGGCGAATTTGTCAATGGTCTGTCCACTTCCAGTAACGGGAGTTTATTACTCTCTGTGGGTCAAAATGGCATTGGTAAGCTCACCAGTATTGACAAATATCGTTTAACCAAGCGGAATGCTAAGGGAGTTAAAACACTACGAGTAACTGCTAAAACTGGTCCGGTTGTGACCACTACGACCGTCTTTGGTAACGAAGATTTGTTAATGATTTCTTCAGCTGGTAAGATTGTCCGGATTAGTCTGGAACAACTTTCAGAACAGGGAAAAAACACTTCTGGTGTTAAACTAATCAAACTAAAAGAGAAGGAACGCTTAGAGACTGTTACTATCTTTAAAAAGGAAGAAGCCATTAAAACCACAACAGCAACCGAGACAGACGATGTTGGATCGAAACAAATTACGCAATAACCTTGACTTCTTTAAAAAGAAGTTAGTTGAACGGGGTGTAAGTGAAAGCCAGTTCGAGGCGTACGTGCAAGCTGATAAAGCAATGCGCAAACTGTTGCACCAAATTGAACTGGCCAACCAAAAGCAAACCCTGTTAGCGCAACAGGTTGCCAAGAAGAAGGGTGATCTGAAGTTATTAAAGGAATCCAAGGAGTTAAAGCAAAAACTAGAACAGCTCAACATCGCTTTTAAGGAAGCGGAAACCTTAAGCCAAGAGTTACTGTTAAACCTCCCTAACATTGCTGATGAATCAGTACCAGTTGGACGGGATGAAACTGCTAACTTAGAACTGCTCAAAGAAGGGCGTAAACCGGTGTTTGACTTTACTCCCTTACCCCACTGGGAGTTGTGTGAACGCTTACAGTTAGTTGCCTTTGATAAGGCCACTAAGCTAACGGGTGCACGGTTTGTCGCTTACACTGATAAAGCAGCGAAACTGCTCCGCGCCATTGCTAGCTTAATGATTGACTTCAACAAAAACAAATACCAGGAGTGAAACGTGCCGGTCATTGTGAACGAGACTAGTCTTACTGGTACGGGTCAGCTACCCAAATTTAAGGATGATGTCTTTAAGTTAGAAAACACGCGTTACTACCTCTCCCCGACCCTAGAGGTGCAACTAGCCAACCTCCATGCCAACGAAATCTTCACGGAGGGTGAACTCCCCAAATACTATACCGCTACTGGGGTGAACTTTCGCCAGGAAGCGGGCAGTGCTGGTAAACAAACTAAGGGTACGATTCGCCTACACCAGTTCCAAAAGGTAGAACTAGTGAAGTTTTGTAAACCAAGTGAAGCGATTCACGAACTGGAAGAAATGACCCGGGACGCTGAGCAAATTTTGTTAGAACTCAAAATTCCCTTTCGCCGTTTGTTACTCTGTAGCGGTGACATGGGTTTTAGTGCCCAGAAAACCTATGACTTGGAAGTGTGGATGGCGGGTTGCAATGAGTACCGCGAAGTTTCGTCCTGTTCTTCTTGTGGTGACTTTCAAGCACGCAGAGCAATGATCCGCTATAAGGACTTAACCACAGGCAAGAATACCTATGTGGCTACCTTAAACGGCACCGCTTTAGCGATTGACCGCATCTTTGCAGCCATCTTGGAACACTACCAAACGAAGGCAGGGGAAGTAATGATTCCCCAAGCCTTGTTGAAATACTTAGACTTTGACAAAATTACCAAACCAAAATAAACCAATGAAACAAGGCGTGTTTGTGGCCATAGAGGGCGTGGATGGTGCGGGGAAAACCGTCCTCCTAGAAGCTTTTAAACAGCGCTTTCCCCAAAGCTTTTTGGGCTTTAAAACGCTTTTTAGCAGGGAACCCGGGGGCACACCGTTAGCGGAAAAAATCCGGGCACTGCTCCTGCATGAAGCAATGGAACCACTTACAGAAGCTTATCTGTTTGCGGCATCGCGCACCGAACACGTGCGCCAGTTAATCCAACCAGCGTTACAACAAAAACAGTTAGTGATAGTAGACCGCTTTGTGTGATCTAGTTATGCATACCAGGGCTTAATTAAAAAAGTCGGACTTGATGTGGTCAAAAAGCTCAATGCTGATGCGGTGGGGGATAGTATGCCCGACTTTACCTTTATTGTTGATTGTGACTTTGAGACTGCTTTAAACCGGATGGCCAAACGCGGTCAGGACAATTTATTGGACAATACTGTCAAAAAGCAAGCGGACTTTAATACTATGCGCCAGTATTACCACAGTTTGGTTGATAACAAACGCGTCTTTCTCTTAGACGGGCAAAACCAAACGGGTTGTCTGGAACAATTTATTGAACAATTAAGCCAATGTTTAACCCAACCCACGCTCTCTTAATTATTCAAAGACGGGGTAGCTATCTCCAACCAGTTTTAACGGAGTACCTAACCCGGGTCGTTTGTGAACAGCAAACGGGGTGTCAAACATGTCCGAGTTGCTTGGAAATTCTCCATGGTACTTACAACAACTTTTACAGTTTTGACCAAGCCAACCCCTTTAAAAGGGAACATGCCTTGCACCTTAGTGAAGTGTTGAACCGCCAAAGTGAATCCAACCAAAAACAGCTGTACCTGATTAAAAACTTAGAAACGCTCACCGCTACGGCCATGAACAGCTTGCTCCGTTTAATTGAGGAACACCCGGTGAATACCTATGGTGTTTTTACCACGAAAAACGAAAACATGATCTTACCCACCATTTTGAGTCGCGTCCAAAAAGTAGTACTCAAAAAAGCGACCCAGTTACCGTTCCAAGTGGACAGTAAGGACCAGGCGATTCTCAAAAGTTTCTTTAGTGTAGATGAACAGTTACAAGCATTGGACAACGGTAGTTTTACCCGCTTAAAAACGATTATTACGACACTTACCAATAAGAAAAACACAGCGAGCACGGTTCACGAGGCTTGGGTGTTGTTTAAACAATTAAACCAAACGGAAACGGCTCAAGTACTGAACTTTATGGTGGATTACACCAAGGACTTAACCAAAAAGGACCGCTTGTTAAACATGGTGCAAAATTTGGTGTTTAACCCACCGAAAGCAGCGTTGTTTGCTAACCTCATTAACTGATAAGATGGACACCAAACAAACCATGTTTGCCCTCGCTACTGCGCCCTTCAACAGTGCCATTCACATTATTAGGTTATCTGGTCCCGATGTTTATCGCATTATTAACCAAATAACCAATAAGGAAGTCAAACCGTTGGGTATGCGGATTCAACGCGTTTGGTTAATTGATCACAACCAGAAAAAAGTGGATGATGTGTTGCTGTTTAAATTTGTCGCACCTAACTCTTACACTGGGGAAGATCTGATTGAAATTTCGTGCCATGGCAGCATGGTAATAGTCAACGAAATTATTGGTTTATTGTTAAAGCACGGGGCGGTACAAGCCCAACCCGGGGAGTTTACGCAGCGCGGCTATCTCAACGGTAAGATGAGTTTAAACCAAGCGGCTTCTGTTAATAACTTAGTACTTTCACCGAATACAACCCTAAAGGATGTCGCTTTAAATGCGTTAGCTGGTCAAGTTGATGCCAGGTTAGAACCCTTAGTGGAAAAGTTGGGTCAACTAGTAATGCAAATGGAAGTGAACTTGGATTACCCCGAGTACACTGATGAACAGCGCGAACTGGTGACAATGAACCAAGCAGTCTTGCAAATAACCCAAATCTTAAACCAAATCGTGGTTGGTCAAGACCAGTTACAACGGCTCAAAGATCCGTTTAAGATTGCCATTATTGGTAATACTAATGTTGGTAAGTCCTCCTTATTGAATGCTCTGTTAGATCAAGATAAAGCGATAGTATCCGCCATTAAAGGCAGTACCCGTGACATTGTTGAGGGTGACTTTGCTCTAAACGGTCACTTCGTCAAAATCCTCGACACAGCGGGAATTCGCCAACACCAAAGTGCGCTCGAAAAAGCGGGCATTCAAAAAACCTTTGGCGCCATTAAAACCGCTAACTTAGTAATTTACCTTTTGGATGCCCGGCAGCCAGAACCTGATCCCAAGATTATTGCGCGATTAAAGAAGCTGAAGAAGGACTTCTTTTTGGTCCACAATAAAGCAGACTTAGTGCAGCAAAGCTTTCAGGTAAGTATTTCTGCCAAACAAAAACAGATCCAACCACTTGTCGATTTATTAACCCAATACCTACACCAGTTTTACAGTGTGGAGCAGAACCAGCTCTACTTAATTAGTGATTGGCAAACGATCCTGCTCCAAAAAGCAATAGCCGAACTAGAACATTTTTTAATTAAACAACAAAACTGCCTTTTCTTTGATATCCTAGTTGTTCATTTACGTGCTGCTCACGAATACATTCTCCAAGTCTTGGGTAAAAACACCAACTATGACCTGATTAACGAAATCTTTAAGCACTTTTGCTTAGGTAAGTAATGGAATACTTTGATGCGCACTGTCACTTAAACTGTGAACCCCTACTGGAACAGCACGAAAAGAGTTTGGCCAACTTTAGGTTAATTGGGTTAAAGGCCAATGTTGTGGGCACTAATTTAACTAACTCCCAAATTGCGGTGAATTTAGCTAAACAGCACCCTGATTTACTCAAGGCAGGGGTGGGTATTCACCCTAATGATGTCCAGCTTTTTGATTTAAAAGCAGCGCAAGCAACACTAGAAAAATTAGTAAGTACGCATCGCTCATTCATTAGTTGTATTGGTGAATATGGCTTTGATTACCACTACACTAAGGACTACATTACCCAACAAGAACAGTTCTTTTTGATGCAGTTCCAACTAGCAGAACAGTACCAGTTAGTGCACATGTTGCATGTCCGTGATGTGCACGAAAGGATATACGAAGTTTTGAAAAGGTTAAAACCTAAACAACCAGTGGTGTTTCACTGTTTTAGTGAAGACACCAATACTGCTTTAAAGCTGCTAACTTTAAGAGAAGTAGGACTAAAGGTTTATTTTTCGATTCCGGGGATTGTGACCTTTAAGAATGCGAAAAACTTGCAAGCCGCTTTATCAGTTATTCCAACAGAGCTACTTTTAAGTGAAACCGATTCGCCTTATTTAGCACCAGTACCTTTTCGGGGCAAAACTAATTGACCGGAATGCGTTGTTCATACAGTGCAAACGATTGCTGACATTAAGCAAGTTCCTTTAGCTGAAATAAAACAAGCGATTGTGCATAACGCCAAGAAGTTATTTTGGTAGTCCTTAGGATTAACTTTTAAAAGTTCTTCTTTTGTTGGAACCTTTTCACAACTTATTCAATTTATTTAGTGTAGACATGAATAAAAATGCATACACTAGAAAAAATTAGTTCCTGATTAAAGGGGAATCCTTATTTAAACAAAATTGTAAGTAGTAGTTTTCTCAACCTCTTTAAAAAGAAAGGAGGTATTGCTTAATGGCTTACTCGCCTTCGCTTAACGACATTAAGAGCATTTTGAACAAATATACCTCTAAGGACTATGAGTTAAAATGCGAAAATAGGTATGATGGCAAGCTTGAATTATGATTAAAAGGAGTTTTTGAAGAAATCGTGAAAACACCTGGCACTAGGTACGTCACCCATAAGCAACTTGATGAAAAGCTCAAAAATTTTGTCACTAAAACAGAATTTAAAGAGTTCCAAACTGTAGTTATGGAGAGTTTTGCTGTTCAAAACCAGAATATTGATGCTCAGGGTGAGCAGATCAAAGAACTTCAAGTTGAACAAAAAGCTCAAGGAAAGACCCTGCAATTGATACTTGAAGCTCTCCAGGGTATAAACAAACGTCTAGACAACTTAGAGTCCAAATAAAAAACTAAAGCAGTTTTTATACCAACAGTTGACTCTTAAAAAGTTTGCTGTCGGTATTTTTATCCACTTCTAACAAAAAATCATTAAAAAACAGGCGACTAAAAAATCTTGAAGAACAACGTTAGGTTGTTCAAAGAATTTTGAACAATAAAAAACAAAACTAAGTTGAATTTTTAGTTAAGCCATTACTCAATTAAATAGACTAAATATCTGAATAGATTAATTTGGTAGTTATCAACTATGTATTCAATATAACCATTAAGAACTGTTTTGTCAAGAATTTTTTGACTAAGTTCATCTTTGCCTTTTAGTATTCAATGAAACGAAACAGATAACCTAATTCTTTTGGAATAGACATCAAAATTTTTTGACGTAAATGGTAAATATGAATTCGCTTTAAGAGTAAGGAAACTGTTGAAATAATGTTTGTCACCTTTGTGGATATTGAATCCTCTGTAAGAATAATTTTCATTTCATGTGAGAGTATGGTTCAACTTGGCTTTAACTGAAACATCGCTAATGCCTTTGTATGTCATGTTGAAATACTCAACTTTTTGTTTCTTATTAAAAGTATTAACGCCTGAAAAAATCGATTTAGTTCAATTAAATAAATCAATACCGATTTCATTTTTTTCTGCACTTTGCTTTCAGGCATTTAGTAATAGTTCAGTAGTTTTTTCAGAGTTAACTTCGTAAGATTTTTTTAAATAACCTATTAAGATTTGTTTCCCTTCCGAAGTAATAATTAATTTAGAAACATCAAGGTCTTTACCATCTGTTTCCTTAATAAGATTTTTGAGATCAGCTTCGAAAGTGGCGCTACAAGCACTAAGAAATAAAACTGAAGATAATAGTGGGGTTAATAAGAATTTAAATTTCATTAGCGCACGTAGTAAATTACTTAATTAAGAAAAGCAAGTATCTAAAGAGATTAACTGTGTATTCAGAATTTGTTCTAGTATCATAGCCTTCCAAAAAGATTTTCTTAAGATCTCTTTGACTTTGTTTATCTTGCCTTGCAATGATCAACTAAGTGAAACAGGGAACTCAATTCATTCTGTGTCAACGCCTTCCCCTGTCTTAGTAATGAAACTTATCGTTTTAGATTGGTTCTTTGAAGATGGCTTAATTGCAAGAGGAAGATCAAAAACATGTTTGTCACCTTTTTTGGCAGCAAACCCATTGGAAGAATAATTTCCATGCCAATTAAAGTTATAGTTCAACTTAGCATTTATCGAAAAATTATTAAGATCTTGATATTCCATATCGAAATATTCAACTTTTAGTTCGATTTTTAAAGGATCATTGCCTGTTGCATAAATACCAACATCGGGAAAATTAATTTTCTCAATATCTATTTCACCTTTTTCCGCACTCTGTTTTCAGGCATCTAACAATAGTTTTGTGGTTTTATCAGGATTAACTTCGTAAGATTTCTTTAGAGAAGTTATTAAGTTTTTTCTGCCTTCAGAAGTTTTGATTACTTTGTACACATCAACGTCTTGTTTAGTGGTTTCTTGAATAAGATTTCTTAGATCAGACTGGATAGAAGCATAAGCACTAAACAATAAAGTTGAAGATAAAAACGGAATTGAAAGGTATTTAAATTTCATTGAGGTACAGAAAAACTAACTATGGCAATAGAACCTTTTTGGTAATGGTAAATTTTATCTAGTGTTTTATTGTTTTTCAACTTAATTCAGTAAAAGCATCCACATCTAAATAAAAAAGCCATCTGTTTAGGGCGAAAAGCAGATGACTAAAAATAATTTTTTAACGATTTAAAAGCTAACGGAGATTGTTAGTTTGATGTATAAGTAAAAATATGTTTTCTAAAAATTTATTAAGTTGGCCGGGAGCAGTAAAAACTTTTGATTCCAGCAGCTCTTCCTGCTCCCACTAAATAAATTGACTTGGTTTTACAAAGACGCCAAAAAGTTTGGAGCTTTTTGTAATTTAGTTTTTTAGCTTCTTCTAACTATTAAGAAATATTTACTTTTCTCAAAAGCTTTTGAAAGGTTTTTGTCACTTCGCTATTTTTAAAGGCATTTAAGAGATGTAAATACATCGCTTATGTTTCTAAATACTCTTAAAAATTTATTTGGGTGACTTGGATCGCTCTTGATGTTGTTTTGTTTGAAGTCTCTTTACTTTAAATTGCAAAAATTTTTAGCAATTTTATTGGCAGTTTTTGAAATTCTGGTCAATTTATTTAGTGAAGGAAAAATTATGTCTTTACTCAATATATTTATTAGATCAACGATGATTTTGATCCTTGCTGGCAACAAAGTTGCACGTTTTAGTTTTCTAAACCTTGAGCAGAAAGGAGGAATTTATTAATGCCTAAAGTTATTTCCCAAAAGGAATTTAATATCCTTTCCCGGACAAAGGAAAGAGTTAATTTTCAAATTGTCTATACCAAGCAAAGAAATGATTTGCACAAAGCTGCTAAGGAGTACTTTTTTAAAGACAAAGACTTTACAATAATAGAAGATACACCGGACACACCTGACCAACCAGATGGACCATGTATTACTAAAGGCCCAAAGGAACCAGGTGGTCCTGAAGAGCCAAATGGTCCGGATAAACCAGAAGATCCTAAAGATCCTGATACGCCAGATGTTTCTGAAGGCCCTAAAGAACAAGGTGGTCAGAAAGATCCAGGTGGTCCGGATGATCCCAATGAAGGAAAGCAGAAAGTGTCCAAACCAGATAAATACGTAACCCACCGTGAGCTTGATGAAAAGCTTAAAGACTTTGCCACCAAAGCTGATTTCAAACGCGTCGAAGACAAAGTTGATGTTTTATTTGAACTGCAAAAAACGCAAGGCGAACAAATAAAGGTTCAAGGTGAGCAGATCAAAGCTCAAGGAAAACAAATTGAACAATTAACTGAAACTGTCAAAGTGCAAGGCGAGCAGATTAGGGCTCAAGGAGAACAAATCAAAGCTCAGAGCGAAGAAATAAAAGAAATTAAGGTTGAGCAAAAAGCCCAAGGCGAGCAAATCAAAGAACTTCAAGTAGAACAAAAAGCCCAAGGTAAAACCCTACAGTTAATTTTAAAGACGCTAGAAAAAATGAACGAGCGTCTAGAAAAATTGGAATCTAAATAGATAACAATCAAAGCAAAAATACCAGCAGTTGACCCTTATAGGTTATCTGTTGGTATTTTTATTTTGGCGCTTACTAGTTCTTGTAACCATTTATTAGTTAAATGGAACTTAGAAATACAAAATTAGAACAACTCTTTTACATCTATCACCGCAACCTGAAGTATAGATGTCCAATGCAGTACCTCACTAAAAGGGGCTTTAACTTACAAGACCTCTTGTCAGTTGGTGGTGGATTAGCTTACTTGGGCGAAAAACAGTGGTTAAATTTATCACTGTACAACTTTAACAACCAGTTAGTTGGTTTTTTAAGTCGGAAGGTAGGTTTTGAAAAGAAGTTCCTTTATTTACCTATTAATAAACCACCAAGTAAAAGTGAATCTTTTTTAGGTTTAAAACACTTACCAACGGAAACGAACACCATTTACCTAGTTGAAGGTGATTTTGATTGGTTAGCTTTCCGTAAGGGTGGTATTTTGAACTGCTTGCCTTTATGTGGATTAACTTTATCAGATAAACAAATGAAGTTTTTTCAACAAAGTAAGATCGAAAAAGTGGTGCTTTGCTTAGACAATGATTTTGCTGGTAAAGTAGCTGCTGCTAACCTGGAACGCATTTTAAAAAATGCTGGTTTTCAGGTTAAGGTAGTTCAGCTAAAGGGTAAAGTTAAGGACTGAAATGAATTATTACTACTTTACCCGAAAAACTGAGCTAAAGCCTTAAGGGATCATTTGGCGCTTTAAATGATGTTTAAAGTGAGTTAAGGACGATTTAATAATTACCCTTGGTAGGTTTAAGTCAGGACACATTTTGGTTAGTGCCCTAGCACCAGCAGCATCCTCGATTTCATTGACCACTATTCGGTTGTACTTATTTAAGAAGAAGTCAATGGCGTAGTAACCATGACCTAGCTTTTTAGCAATCCGTTTAATTTTGTGCTTCATGTATCACTTTAGTTTAAACAAATCAACAGCAGCATTTTGTGAGTAGTTAGCCCTAAAATCATCACCACTAGCAGTCCTTCTAATTGTTTTTAACACCTTACCAAACAAGATGTAAGCCCGGTATTCCACTTTACCAATTGACAAAAATGGTTGGACAATCCACTCATTAGGATTTTGAATTGGTAACTGCTTAATTTCTTCCGCATTTTGAATTAAGTGCACATCTTCCCCACCATGAGAATCACGCTTTTTGATCACAATCGGAAAAGTCTTAATCTTTTTGGGATCAAAAATGGTGGTATCAACGGTCAAGAACTTATGCTGCTTTAACCACCTGTAAGTTTCATACTTATCATTGGCTACCAAAACGATATTTTGGGGATTAATGAGAAAACTGTTGAGAAAGTTGTGTTTGGTTAAAAAATCAATTCTGCGCGAACGGTTAATGATCAATTCCACATTTTTAATGTGTTTGTGATCAGCGGTTTCATCATCTAACAGCAAAATATGCCCAACTAGTTTTTTCTTGTTCAGTTCATCGACTAGTGCTTGAGCAAAGTGCTTGTTCTTGTCAAAATCGGTCTTATTGTAAACAACGAGTGCTTTTCTGTTTTTTAACTTAATTTTGGCCATTATGCGATTGATGCTAAAAGTTGAATGGCGAGGTTAGTTTTCAAATACATCGAGCTTTTGGTGAGCTGTACATTGGAATTAACTTCGCAGAAAATTAACGAACGATCCTGGTCAAATAAAAAGTCAATGCCACAATAAAAGAGTTGCATTGCTTGGCTAATTGTAATTGCTAATTTGGTTTCTTCCTGACTTAGTTGATATGGTTCACCAACCGCTCCCTTATTTAAATTAGAGCGAAAATCACCCTCATTTTGAGTATGTAAGGCCCCAACTACCTGGTTATTGACCACAATTACCCTGACCGCTTCACTATTAGAAGTGGCAATGTATTGCTGCACAATGATCTGTTCAGTTAGCCCGCTTAAGGTTTGACGTAACTTCTGTTCATCTTTGCACAAAAAAACGTAATCACCAAAAGAACCATAGATACTTTTGACAATCACTGGAAACTGAAAACAGCGCTTAATTTGTTCGATGAATTGTTCCAACTTTTCCGGATATCAAGCTAAACGAAAGTTCTGTGGGCCAATTAATGTAGGAATCTGTTTAATGGTTGGGTGTTGCGCTAAAACAGCATGACTCAAGGCCTTATTGTCCGCGTTATTAATGGCAATCGAACTGTTAATAACGCGCAAACCAACTGATTCTAACCATTGCGCTAACGCTATGTTTTTATCCAAAAACAAAATGGTATTGGACTTAAAGGGTAGTTTGGTCTTATCCTGATTGGTATTAATATCAAAGCAAGTAAAGAAATCGAGTTGGATGTTTAAGCTCTGGGCTGCATCCTTTAACAGTTCCGTTTGGTTTAATTTGGAAGCAATGTTAGTAAAAGCAGGGTTATATACGGCGCTGATGTGCTTCATAAAGGACTCAAAGGTTTTTCATAATGGCTAACACAGTAAAGGGTCCTACCCCTCCAGGTGTGGGGCTTCACCATTTGGCAACTTTCTCTATCCCATTAGGATCGATGTCACCACACAGTTTGTTTAAGGCATTACGTGATACCCCCACATCATAAAGAATAACACCTGGTTTAAAGTTAGTGGTCTTAAAGAAATGGGGCTTGCCAATGGCTGTGATCACCACGTCAGCGGTTTTAATTAGCTCCATGGTGTTGGGGGTATGCTTGTCACATCCCACCACCTGATGACCCGTTTCCTTTAAATAGTGAAAGATCGGCCCGCCACCAGTGACGCCTAAACCCACCACCACAATCTGCTTGTTGGTGAAATCATGGTGAAAGTGTTCTTTCAAAATTAACACCGCTTCCACCACACAGGACCTAATGGGTAGGGGGCAGTTACCAGTTAAGTAATCCACATCCTTGTAAGGATCAATTGCTTTTAAGAGCTTGTTTTGTTTTAAATGGGGATACACTGGCAGTTGCAAGATAATCCCTGTTACCTCAGGATCAGTGTTGAGTTGTTGAATTTTTTCTAACAATTCTTTCTCAGTGACACTTTCGTGGTACTTAATTAGTTCACTCTGGGCCCCTAGAGTATTACAAGCATTCATTTTTTGGCGAATAAAGGCATCAGATGACACATCATTATTAGTTTGCAGAATGACTAATTTGACCTGACTTCAATCCCTGTTTTTGTAAGTTAAAAGGATTTCGTGTGCTAGTTGCTTGCCATCAAAGGACATCTCTTAAGTTGTTGTTTTTTGTCAAGCTAAATAAAAAACGCCTAACTTAATCTTATATGACTAGTTAGACGTTTGGATCTTTAATGGTGGAGATGAAGGGTCTCAAACCCTCAACCTCCTGAGTGCAAATCAGGTGCTCTATCAGTTGAGCTACATCCCCATTATTGGTGGAAGTAAATGGACTTGAACCATCGACCTCACCCTTATCAGGGGTGTGCTCTAACCAACTGAGCTATACTTCCAGGCAAAATCTTCGTACAGGTTCGCTTCATAATTATATTAATTTAGCCAAAGCTAGTTTTTAAGCGGTCTTATAATTAAAGCAAGTAGTTTTTATGGGTCTTGTTTTAAAACAATTTAACAGGAAAATAAGAACAGCTTTAATTCTGGCGCCGCTTTTCACCTTTGCACAGATTATTATTGATCTTATTATTCCGTCCTTTTTGGCCAGTGCTATTGCCGTAGTGTTCTCAATTGTCACGCTTAAACAAAAAGAAGCATCAGGCGAGGGAGTTGCTGTTGATTTTATTGCTGAAAGTAAGTTAAGTTTCCAAAGTGTCCAGGAAGCGCAAATAGTGTTAGCGACCTCAGTGATCTTGTTAGCACTCTTTGGTTTAGTGTTTGGGTTAATTTCCATTTTTTGTGCCAGCATAGTAGCGGGGAATACCTCTTACTTTTTAAGAAGGAAGATTTTTCGCAAGATTATGCACATTACCGCACCTTCTCATGACCAATATGGCTCTTCTACGTTGTTGGTAAGGTTGACCAATGACGTTTACTTAATGGAAATCATGACCTTTGATTTTTTGCGGTTAATTGTTCGTGCACCTTTCTTGTTTATAGGGGGTCTAGCCTTTGCTATTGCCACTAACTCTGACATGTCGATTTCGCTAGCCATTACCTTTCCTTTGACCTTCTTAGTGATTGGGATTCTCAACAAGAAATCGACCCCGTTATTTAAGAACAACCAGAAATCGGTGGACCAAATTAACGAACGCGTGGAGGAAGATGTGTCGGGTTACAAGGTAGTGCAGTCGTTTAACTTAAAGGACACGGAATGTCTCAAGTTTAAGGCAGCTAATGCAAGGTGAACTAAGTCGAGTACGAATTCGTTGTTTGTCAATACACTGAACATTCCCTTTACCTTCTTCTTTTCCAGTATGACTATCATCATTGCGTTGCTTTTAGTCTTCCAACTAGATAATTCAGTCAGAGTGGATCCCCTACCAGATAATGCTGCCATTAGACCCAGCATCTTTGCTTTCTTCCAGTACAACTTCTATATTGTCTTGGGTTTAATCTTGACCTCGCTAACAATGGTCAACTTTACCCGTTCCCGCGTGGCCTTGGGTCGGATTAAGGATGTTTTGAATAAACCGGAAATCCAACAACATGTGGTTCCTGATCAAACAGTTCTAGCCCCTAGCTTGGAATTCAAAAACGTGGCCTTTGGGTTAGGTAACAAGGAAAACCGGGACTTCTTGCAAGACCTTAACTTTAAGTTTGAGGCCGGTAAGACTTACGGAATTGTGGGACCTACTGGTTCGGGTAAGTCTTTAATAGCTAATATTATTGGGGGTTTATATGAACCCAACCAAGGTGAAATCTTTGTTGGTGGTCAAAGTATTAAAACGATTGACAGTGACTATTTAGCCAAGATGATCGGGATTGTCTTTCAGCAAAACATTCTCTTTAAGGGCACGATTGCTTCCAACATTAAGATAGGCCTGGAAACCAGGGAAGACTGGAAGCACGAACCAGATAGCAAAAAGGATGCTGCCATGAAACGCGCTGCTGCCATTGCTTGTGCTGATACCTTCATCGAAAAGTTCAGCGATACCTATGACCATACGGTGGAACAGTTAGGCAAAAACCTCTCTGGTGGGCAAAAACAACGCGTCGCCATTACTAGAACCGTTATTACTAAACCACAAATCTTAGTGTTGGATGACAGTATGAGTGCGCTGGACGCTTTAACCGAAAAGAAGGTCCGGGAAAACATTGCCAACGAACTTCAGGGCACCACTAAGATCATTATTAGTCAAAACATTAATTCGATTAAGTACGCGCACAAAATTATGGTGATTGATAACGGTCGGATTGCTGGGTTTGACAGTGATGCTAAGCTCATGCAGAGCTGTGACATCTATGTCAAGATGAAGCAAGCACAAAAAGACCAAGGAGGTGATTTCGATGCTGTCGCATAACCAAAAACCCAATTCCTGGAAGATTTTGTGACGTTTAATTAAGTCCGTGCAAGGTCGCACTTCCTCCAAGGTGCTGTATGTCATGGTCTGTGCCATCTTTGGTATTCTCACTGGGGTTACTAACTCGATTCTCTTGGCCCAGGGCCTGGGTTTTATTTTTCCTACCACTAATACCGAAACTGATGGTATCCAATCAGTGTACTTACTGGTATTTGCGCACAACTTACCGGTCATGGAGCGCTTGACGATTGTTTGTGTTACCGTGGTTGTGGCTTACATTCTCATCTTTAGTTTTAACGTAGCGCAGAACTATTTAGGTTTAAAGCTGTACCAAGAGATCTGTGCCTTGTTGCGCTGAAAGGCTTACCTCAAGATTCAAAGTATGTCCACTAGTTTCTTTGATACGCAGAACAACGGGGATTTAATGAGTCGCTTGACCAACGACGTTTACAACATTAACAACCTGTATGCCCAAGTGGGTGGACAGACAATTCAGAGTTTGTTTATCTTAATGACAACAGCGACGATTCTGTTTGTTTTAAGTCCAGTCATTGCCTTAATTTCGCTAACAGTCTTAATTGCTCTAATTGCCCTTTCCTTTCTCTTTTTAAAGAAGGCACGCGCTGCTTATGCAAAGGTGCAAAACAACTTAGGGGACATGTCGGGTTACATTGAAGAAGTGTTGTCGAACCATAAGGTAGTTCATGTCTTGAAACTGCAAGAGGTGATGATTGATAACTTTGACAAGTACAACCGCCCGATGGTTAACCCCACCATTAAGGCCAATACCTATGCGGTGTTTATTTACTCCTGGTTTGGTTTTATTTCCAATATTACTTACTTAGCTTCGATCTCAATTGCCACGGCCTTTAGTGTGAATAACATTCCCTCCTTTGGGGTGAGTGCGATTAACTATTCGTTTATGCTGTCTTACATTGCGGCCTTACGTCAAACAGCACTCCCTTTAAACCAGATCTTTAGCTTGTGAAACTTAATCCAGTTAGGGATAGTAAGTGGCGAGAGGGTCTTCAAGATCTTGGATCTAGAAAGTCCCCAAAAACAGGCCACCATTACTAAGTTACCCAACATTAAGGGCAACATCCGTTTTGAAAAGGTAGCTTTTGGTTACAGTGCGGACAAACCAATTTTAACGGGGATTGATTTCTCGGTTAAACACGGTGACATTGTGGCCATAGTCGGTCCTACTGGAGCGGGTAAGTCCACCATTATTAACCTGTTGATGAAGTTCTACAAACCGTTTGCTGGGAAGATCTACATGGACAACTTTGAGATTAGTGAGGTGAGTGAAACTGCTTGACGGGAAAAGATTTCGATTGTGCTCCAAGACCCCTTCTTGTTTAGTGGCACGATTAAGGAAAACATCCGCATGGGACGGCAAGATGCCACTGATGAGGAAATTATAGAGGCGTGCAAGGTGGCCAATGCTCATGACTTTATTATGCGTTTACCCCAAGGTTACAACACCTTTATTTCCAACAAAACTGATTATCTCTCGGTCGGTGAGCGCCAACTGTTAACTATTGCCCGCGCTGTTATTCGTAATGCCCCGGTGTTATTATTGGATGAAGCCACGAGCTCCATCGATGTACACTCCGAAAAATTGATCCAACAATCAATAGGCAGACTAATGAAGGATAAAACATCGTTTATAATATCCCATCGACTCTCAATCATCCGTAATGCGACGCTGATAATTGTGATTAATGATGGAAAAGTTTTGGAAATGGGTAACCATGAACAATTAATGCGGCAAAACGGTTTTTATGCTCGATTAAAGCGTTCTGCTGTGAAGTAAGATCCAACCATGACGATTGCTGAAATAAGGAAATTTGCACAAACCACGCAAAAGTTTGTCGAAGCGGAAAACCTGTTTGAGCATGGTAACGTAGTCTTGCCCAAAAAATACCTCAATAAGGCGCGGGGCATGGCTGAAGTATTGTACAACAGCCAGGTCATTAAAGTAAGTTTTACGGCTAAAGATGGTGAGCTAACGTGTAAGTGTAGTTGTTTGGCTAACGTTGACAACTGCGTGCACATTGTAGCTGTATTGCTCAAATACCACCAAATGCTAGTGGAGTCCAAACGCAGTTTTAACTTAGCTGAAGCCTTTCACTTGGACTGTGATCAGGCCGAAATGCTGATTGAAAACCTCAGTTTAGAGATTATTGCCGGGGGATGAAACTTTAAGCTGGGGTTCACGATTAACTTAGATAAGCACAATCCCCAACCGAGTGTCTTGCGCTTTTATTGTTGTGATGCGACCTTTGTGTACTTCCTTCACTTGGAAAACGACACTTTGCACTCAGTCGAATTGTCCAAGTTTAAACCAGAGGAACGCGCTACCTTACTCTTCTTTGATAAATTGTGCAAGCAGTTTACAGTGGGGTATGACCGCAACAGTTTGCTCTTTCCGTTAGCGGGTTTTTTAAAGGAGTTACAAGCCAACACGGAGCCCACCATCTTTGTCTTTAACGATGACAAAATTGACAACATCCTCTTTTTAAGGATTTCCAAAAAGCACCATGGTTTAAACCATGTTTGTGGTTTTAGTGGCAAGAAGGTATTTGACTTTGTTACTTATAAACAAAAGGAAAAGCAGATTGTGTTGCGCTCAGCGTACCTGAGTAAGTTCACTGACTTTAAGTTTCCTTACACCATTAACATCTACAAACTCCAGTTTGGGGAACCGCTGTTCTTTCACTTTTTAATTCAGCTAAAGCGTGATGGCTTTAAGAACTTCTACTTTCAAAGTGAAGACAGTATTGTCAAGGAAAAGGAATACCTACCGAAGCTGTACTTTAAGGTCGAGTACGATCCGGTCAAAAACAAGTTTGTCAGTGATGCCTTCTTTAAGTACAAAAACCACTTTAATAAGGGCACAACCACGGTGTATCCGCACCGCTATTACATGGCTAAAAAGTCGGATCGCGGTGGTTTTAACCGCTTGTTGTTTTACGAAGAGGCCGTGGAAAACTTCTACCAAGACCAGTTTGATTTGGGTTACTTCCGTAAGTTTGAACACCTACCGATCCAGGACAAAAACCAGATTGAGGCCTTTAAAGCAGCGCTAGATGACTTAATGCCCGTGGATTTGGCTGAAGTGAGTTTATCCGATAACTTACTCCACCAAAAACCGCTTCATTTTGCCCTGTCTGATCTAGAAGCAGTTGCTGTTGATGACAAACAAATTAAGTTAAGTTTTGCCCCTAGTGCGGTTGAACTGAAATTAATTAAACGGATCTTATCCGCTTACCATAAGGGCAATGTGGTCTGTATCGACCAGGAGAGTTGGTATGACCTCAAGCAACCAGCTGCTAAAGAACTAATCCAGTTCTGAAACCAGTTTGACTTACGCAACGCTACCAGTGATGGTAATCACATCTACCTCCCGAAATATTACTTGTTTGAAGTGGCCAAGATCTTTAGTCAGTACTTGGACATTAAAAACCTGTTTGATGTACCGACCATTAAAAAGATTGAAGACCAAAACAACAACGTCTTTGACCTTTCCTTAGAACACAAAAAGATTACTTCACTGCGCAACTACCAACAAGAAGGAGTGAAGTGAATTCGCGGTTTGGAGGAAAATAAGTTTGGTGGTATTCTGGCTGACGAAATGGGGTTAGGGAAAACGGTGCAGGTGATCTTTGCGCTGTTGGACAGCTATCTCAAAAACCACGTTAACCTCCCTAGCTTAATTATTGTTCCGGCTTCTTTATTGTTGAACTGAAAGAGTGAGTTTGAAAAGTTTGCCCCGCAAATTAAGGTCAAGGTAGCTAACATCCCTTCTAAGGAACGCGGTGAGTTGTACGAAAAACTTACTAACGAAATCCTGATTGTGAGCTTTAACGTGTTGCGCAGTGATGTGAAGTTAATTACCAAACAGCGCTTTCACTATGTTGTAATTGACGAAGCGCAGGGCATTAAAAACGACAGCTCTTCAATTACCAAAGCTGCCAAAAAGGTGAAGGGCAACTTCTGTTTGGCCTTAACTGGTACGCCAATAGAAAACCGCTTGTTGGACCTTTGATCCTGCTTTGATTTTGTGCTCCCAAGCTTTTTAGGGAACAAAAAGCAGTTTACCGACCAGTTTGAAAAGGAAAAAACCGACCAAAGCTTCCATTTGTTAATGCAGCGCACTAGTCCCTTTATTTTGCGTCGTACCAAGTCCAAGGTCTTGAAGGAACTGCCTAACAAAATTACCACCGATATTTACGTAGAATTAAACCCAATGCACCAGAAGCTGTATGAGGAAGAACGTGACCGCGGTTTGGAGGAAATTAAGCAAATCCAAGACAAGAGTTCCTTTAACATCTTGACTTTAATCTTGAAGTTAAGGCACTTGTGTAGTTTACCCAAAAACAGTCAGGGTATTTTGGAAAACAGTGCCAAAAAGGAAGCTGCTTTAGAAATTATCCACGAAGCGATTGAAAACCAGCGCAAGATTATCCTCTTTACCCAGTTCATTGATGTAATTGACCACTTTAAAGACACCTTTAAAGAGCAGGGCATTGAGTACTTTATCTTTGATGGCCGTAAGTCTCCTAAAAGCCGTCACAGTATTATCGAGAAGTTCAACAATGCCAAAAACCCTTGTGTGTTGTTAGCGTCCTTGAAAGCAGGGGGTGTGGGAATTAACCTCACTGCCGCTGAAGTGGTAATCCACTTTGATGTGTGGTGAAATACCGCCGTGGAAAACCAAGCGACTGACCGGGCCCACCGCATAGGACAAAAGAAAACAGTCCAAGTTTACCGCATTATTGCTAAAAATACGATTGAAGAGCGGGTCTGTCAAGTGCAGGCCGAAAAACAGGAGTTAGTGAGCAAAACGCTAGTGGAAGACGTCAATTTTTTCGAATCCTTGACCAATGAGGAATTATTACGTCTTTTTGAATAAATCAAAGCAGTTTTATAATTAACACTCGAATGATTAAAGTGATAAATGGCCGCTGGTAAACGTGATTATTATGAAGTGTTAGGCGTTTCCAGATTCGCCACCGCACAAGACATCAAACGAGCTTTCCGCAAGCTTGCTATGCAGTACCACCCCGACCGCCATAAGGGTGAGGGTGAGACTGTCCAAAAACAAAACGAAGAGAAGTTTAAAGAGGTCAATGAGGCGTACGAGGTCTTAAGTGACACCGAGAAGCGTGGTATGTACGACCGCTTTGGCCATGAAGGGTTAAATGCCTCTGGCTTCCATGAAACTGGGTTTAATCCCTTTGACATCTTTAACAGTGTCTTTGGTGAGGGCTTTTCGTTTGACATGGACGGGGGTTCACCGTTTGACTTTATCTTTAGTAGGGGCAAAAAGTCGCGGCAAAACCGGGTTGTTTTGCCCTATGATTTAGAGATTGCGGTGGGGGTAGACATTAGCTTCTTTGAGATGACTAATGGTTGTACTCGGACGATTGAATATACCAAACGGGTGACCTGTAGTGCCTGTGATGGTTTTGGGGCTGAAGGGGGAGAAACCGGGATGGTTAGCTGTAACAGTTGTGAAGGCAATGGTTTTATTTTGAAGAATCAACGCTCCATCTTTGGTACAGTCCAGTCCCAGATGTTGTGTCAGAGCTGTGGTGGTCAGGGTAAACAGGCCAAACACAAGTGTAAGACTTGTAAGGGAAGTAAGTACAAGAAGGTACCAACTACCAAGGAAATTCAGATTCCCGCTGGCATTTACCATGGTGATGCGTTAGTCGATGACCATGGTGGTAATGAATTCGGGGGTCACGTTGGCAAGTTAGTGGTCCATGTGGGGGTACTACCGAGTAAGATCTTTAAACGGGTTGACCATAATGTAGTAGCCAATGTTTTAGTCGATCCGATGATTGCTATCACCGGAGGGAAAATTGAACTCCCTACCTTAGAAGGCATTAAGGAATTCAACTTACGTGCTGGTACTAAGAGTGGCGAGCAAATAGTAATCCCTGGTGGTGGGATTAAATTTACGAAGAACTTCCGCAAGAAGGCCGGGGATCTCATTATTATTATCAGTTATGCCCGTCCAAGTGAATACAGTGCGCCAGAGTTGCGCAAGCTCAAGGAATTTATTAAACCTAATCAAGAGGTAAAACAATATTTAAACGCACTTAAGAATGAATACAAGTCCTAAGCAAAGTGGTTATTTAAAGGTTGGTAATGGTCATGAGGTCTACTTTTGAACTGCTGGTAATCCCCAAGGAAAATCAGCACTTTACGTTCATGGGGGACCAGGTTCGGGTACGGATGCGGGTTGTTTAAAGTACTTTGACTTAGACACTACCTATGTAATCTTGTTAGACCAACGGGGTTGTGGTCAAAGTAAAGCGGTTAATCCCTTACTGCACAACACTACCCAAGACTTAGTTGGGGACTTGGAAGCGTTGCGCCAACACTTAAAACTTGAGCGTTGGACCCTATTTGGTGGTAGTTGGGGTTCTACTTTAGCCTTAGTGTATGCCATTACCCATCCCCAAGTAGTCGAACAAGTCTTTTTAAGAGCGCTCTTTTTGGGCCGTGAACAAGACTGGGCTGAAATGCTGTTGGGCTTGGGCAAGCTCTTCTACCCTTACGAACACCAAACGTTACTGAAAGCCATTCCCCAGGCTTGTCGCACTGACTTTACGAAATTCACTAACTACTTTTATGAAGTGTTGCAAGGTAACGATTCCGCTTTAAAAACGCAGTTAGCCAACGCTTGGGTTAAGTGGGAAAATACCTTACTATCACCGATTAGCTATGTCAAAGACGAGAAAGCAGAAGATGCGAACTTTACCTTTAAGTTAGCGCTCTTGGAATGTCACTACGCAAAGCACCACAGCTTTTTAAAACCGAACTTTATCCTGGAAAATGTTGCGGTATTAAAGGACAAACCGGTGCACTTAATCCACGGCCGTTTTGATCTAGTCTGTCCGTTAAGTCAAGCCTTGGAACTCAAACGTGCTTTACCAACGCTTAACTTGTATGTAACTAACAATGCCGGGCACAGTGGCAGTGATCCCAATAACCTCACTACCATTAAACACCTTTTAAAAACACAACTGTAATGAAACGGTGTTACATTACCACCCCGATTTACTACGCCTCGGGTAAGCCCCACATTGGCCATGCCTTTACGACCATCTTAGCGGATGTGATTAAGCGGTATAAACAGCAAAACGGTTATGAAGCTTACTTTCTAACTGGTACGGATGAACACGGCAACAAAATTGAAAGTAAGGCCAAAAGTTTAGGTCTAGATCCCCAAACCTTTGTGGATCAAAACGTGGCTTACTTTCAACAAATGTGAAAGCAGTTAGACATTAACTTTGACCACTTTATCCGCACCACTGATCTTAGTCATAAAGCCCAAGTTCAACAGGCCTTTCAACTACTTTATGACAAGGGGTTAATTTACCAAAGTAACTGGGAGGGTGCTTACTGTGTCGAGTGTGAGCAGAACTATTTCACTTATGATAAACAAACAATGTTGTGTGAAATAGGTCACCAGTTAACCCTAGTCCAAGAACCATCTTTATTTATTGCCTTTAAAGATAGTAAGGACTGGATTGGGGAAATGATTGCGACAAACAAGCTCAACATTACCCCTGAATCCAGAGCCGCTGAGTTAAAGAACAACTTCTTGGATGGGGGTTTAAATGACTTAGCTTTAACACGGCAAAACGTGACTTGGGGAATTCCCGTACCCTTTGACAACAAGCAAACGATTTACGTGTGGTTTGATGCCTTGTTTAACTACATTACTAACTTAGGGTTTGCCCATAACGACCCTAAATTTAACAAATGGTGGAACAATAATGATGAGGAACACGAAGTCATTCATTTAATTTCACGCGAAATTACCCGTTTCCACTGCATTTACTGACCAATCTTTCTCCACCAGTTGGGTTTTAAACTACCGACCCAGTTTCTCTCCCATGGTTGGATTGTCGATGGTAATGGGCACAAGATGTCCAAGTCGTTGGGCAATGTCATTTCCCCCGAAGAACTATTGGCCCAGTTTGGTGTTGATGGTACGCGTTATTGCTTACTTAAAGAAATGCGCTTGGACAAGGACAACCGCTGTAGTATGGCGATCTTTAAGGACATTTACAACGCTGATTTGGCCAACAGTTTTGGTAACCACGCATCACGTACCTTTGGCATGATCAAAAAGTACTTGGGCGGCCAGTTAGACTTTATCGAAGTCCAAGACCCTCAAGTAAAGCAGCTAATGGACCAAGCGGACCAAGCGATGGTTCAGTTTGACACCGCTTGAAATAACTTCCAGTTTTACAAGGGCATTAATGGCTTACTGCAACTAGTCTTTCAAGCTAGCAAGTTAATTGACCAGTTAAAACCGTGGGAGTTAGTCAAACAAACAGATTACACCTTACTCAAGCAATTACTGTTTGCTTGTGTCCGTTGTACCCAAGTGTGCTTTGTCTTGTTAGCGCCGATCTTAGTTCATACCAGTACCCAGATATTTGACCTGTTTAACTTCAGTGCCCAAGCACGTAGTAAGACCCACCTAGCTGATCCACAACAACTGCAAAAAATCAGCTTAGCACCTGTAATTCAACTTTTATTTAAAAGATTGGATTAATTAAATTAGATTAGAATTTAATGACTATGCAAGTAGAATACCTCGATCTCATCTCCCAAGCGAAGGAGATTGCTGAGACCCAATTTAAGGCGGAACCGTTTTCCTTTGATGCTATTTGAAAGGAAGTAGTGAAGCACTTTAAGATCTCCAAACAAGACGAACCTAACCTAATTTCGCGGTTTTACCAAGACTTTTTGGAAGACCCTAACTTTGTTTATTTAGGGGAACGCAACTGGAAGTTAAGGGACTTCATGAAGTTTGATGAATGGAACAAGATTTCCCAAGCAATGTTTGTGACCAAGGAAATCTTTGAAGAGGGTTATGAAGACCTGTCCAACAAGAAGAAGGAAAACGAGGAAGAAGTGAATGACTTCATTATGGGCAATGATGGTGATGACAACAGTACCGGTGATGAAATTGTGCAAGGACTCATTAACGATTTTCGCGACGATAAACAATAATGCTAGTAAACATCAAACAAATGTTGCAACACGCTAAGCAACACCACTATGCTGTACCCCACATTAATATCAATAACTATGAATGGGCTAAGGCAGTATTAACAGCGGCTCAGCAGGCCAAGAGTCCGATTATTGTCTCCACTTCTGAGGGCGCTTTAAAGTATATGTCCGGACACCAAGTGGTAGTACCGATGGTAAAGGGCTTAGTCGATGCTTTAAAGATTACTGTACCCGTAGCACTCCACTTAGACCATGGTAGTTATGAAGGTTGTAAGGCAGCGTTGCAAGCTGGGTTTAGTTCCATTATGTTTGATGGCTCGCATTTACCCTTTCAAGAAAACTTCACTAAATCCAAGGAGTTAATTGAACTAGCTAAACAAACCAATGCTTCTGTGGAACTAGAAGTGGGTACCTTAGGTGGGGAAGAAGATGGTATTGTGGGTCAAGGTGAATTGGCCAACATTGAAGAGTGCAAGCAAATTGCGACCTTAAAACCGGATGCCTTAGCTGCTGGGATTGGTAACATCCACGGGCTTTACCCCGACAACTGAAAGGGTTTAAACTATGAACTAATTGAAGCGATTGCTAAAGCGACTAACTTACCCTTAGTGCTCCATGGTGGTTCGGGAATTCCGGAAGCGGATGTCAAAAAAGCAATTGGCTTAGGCATTAGCAAGCTCAACATTAACACTGAATGTCAACTAGCGTTTGCTAAAGCGATTCGGGAATATGTGGAAGCCAAGAAGGACTTAGATACCCACAATAAGGGCTATGACCCACGGAAATTGTTAAAGTCACCTACCCAAGCAATTGTGGACTGCTGCTTGGAAAAGATGCAGCTCTGTGGTTCAACCAATAAGGCTTAGATGTTAAGTGCGGGAATTGTTGGTTTACCCAACGTTGGTAAATCAACCTTGTTTAGTGCTATTACCAACCTGCAAGTGGAAATAGCTAACTATCCCTTTGCCACGATTGAACCGAATGCCGGGATAGTTAACGTAATCGATGAGCGCTTAGACAAACTAGCGAGTTTAATTAAACCCGATAAGGTTACCCACACTACCTTTCGCTTTGTTGACATTGCTGGTTTAGTTAAGGGTGCCAGTAAGGGAGAAGGACTGGGCAACCAGTTCTTGGCCAACATCAGAGAAGTTGACCTTATTTGTCACGTAGTGCGTTGCTATGAGGACAAGAAGATTGTCCACGTTAATAACCAGGTCGACCCCGTATTTGACTTTGAAATTATTGTCAATGAGTTAATCCAGGCCGACATTGAGGTGGTGAATACCCGGATTGGCAAGATTAAGCGCAAGGCCGAATCTGGTGATAAGCAGTCCAAGGAAGAATATCAACTGCTAGCACCCGTTTTACAAGGTTTGCAACAAAACCAGATGGTGTTGCATCTAGTGAATGAAGTAGATTTAAAGAAACTCAAGTCACTTAATTTACTAACCGCCAAACCGATCCTAGTGGTGGCCAATGTGTCAGAAGCGGATTTGTCCAACCTTGACCATAATCCCCACCTCACTCAGCTCAACCAGTTCTTAAAACAACATAACTTACCCCATGCCATTCCTGTTTGTGCATTATTAGAAAATGAGTTGAGTAGCTTGGATGCCAATGGTCGTCAAGACTGGCTCAAGGAGTTGGGTTTAAGTGATTACCAAGGTTTAAACCAACTAATTAAAACGGCTTATGATGCGATTGGTCTGTGGTCATTCTTTACCTTTGGTAAGCAAGAAGTGCGCGCTTGGGCTTTTAAAAAGGGTTGGTTAGCACCGCAGTGTGCGGGTGAAATTCACACTGACTTTGAACGTGGTTTTATTAAAGTAGAAGTAATTAGTTGAAACCAACTATACGAATTAAAATCGCTCCAGGAAGCCAAAAAGCAGGGCCTGGTCCGTTTAGAGGGCAAGGCTTACGTGATGCAAGATGGTGATGTGTGTCACTTTAAGTTTAACGTTTAGCAAGTAACTGCTGAAACGAGTAAACAACCCAACTTTGCTTGTGGTTGTAACTAATACATAAACAACACAACTCATAGTTAAAAAAGACTTCCACCTGCCCCTTAGTTAACTTGTGGGTTTTAAATCAAGCTAACAGCGCTTTTTGACTGTCGGAATAGGCATCAAAACCAATGTTAATTAGGTTAACGGTGAGGTTCTTAGTTGGTTTTAACATTTGGTTAACCTTTCACCCTGACCCTCAACCAAGATAGGGTTTGGTTTCTATGACTGACTCCCCTTTAAAGTCCTTGGTGCCAAAGTACACCTTCGGGGCATTGGGCTCAGCTTTCTTAAAGTAAAACAGGGAAGGAAAACCCAGTTTGTTAGCGAGGTGCCGTGACCTTTGGTTGGATTCATAAAACGATCACACTAATTCCCGCGGTTGCAACGTACGCTGGTAGTACTTAAGAAAGAAACTAATTGTTTCTAAAGCGACACCACTTTGTTGTTGACTGGGATCAATCCAAAACGATAATACCGGCATGTTGCCCCATTGCCCGACACAAAACCAACCCAAAAACTGCTTGTTTTTAAATAAGAGCGCTAACAGTTTACCGGGTTGCAGTTTGTCGTGGATGTATTCCAAGAACTGCTCAAAGTTTTCCACAATTTGCGCCATATAGTTAGTAGCACTTAAGGTGGTCCGGTGCTTCATGAAAACATTAAAAGCAGCTTTGACCTGTTTATTTTTGACCAACTTATTGGGGTTTTTCAGTGCCATTAGTTGGTAACGATCACCAAAAAAGCAAAACTCAAGAATAGTGTTTTTATCCATTTCAAAAGTCTTTTATAATCTTAAAGATAGTTGTGCAATTCAAGTATTTATTCACCGTGATTATTCCCACTTACAATTGTGGTCAATACATCCCAAAAGCATTAGATTCCTTGCTTTTACAAGGTGAATATTTTACTAAGACCCAAGTTTTAATCGTCAATGATGGTTCGACTGATAATACCAAGCAGATCGTAGAACCCTATACCCAACAATACAGTAACATTGAGTACTTGGAAAAACCCAATGGCAATTGAGGCAGTGTGGTCAACTTTGTCAAACAAAACCAGTTAGCTAAGGGTCAGTACATTACTGTATTAGACAGCGATGATTATTTTCTAGCTAACGCTTTTCAACGCGTAGCTGCTCACTTTGGCCATGACATGATTGTGAGCGCCTTTTACTGTTACATTAGCCCTAAAAGAAGGCGTTTTCTCAAACCATACTTTGGTAAAACGGGGGTAATTGAACAAAAGACTAAACTACGCACACCCCATTCCCAACCCTTGGCTAAGTTCTACCGCCACGAGATCTTTAACTTGCTAGATCCTTTAAAAGAAAAGCTGTTTTACCAGGACTGTCTGTTGTACCATAATGCCATCAATAAGGTGCAAAGTGTCTTTTACATTTGTGAACCCTTAGCAGTGTGGTATGCCACTAGACCAGGTAATTCCACCACTATGCCCTGAAATAATGCCGATAAGTTCCAGGCCTGGTGTGACTTGCTCAAACAAATGAACTTATACGGCGCGGGTATAGTGATTTACATTTACACTATGTTACCTGGTTTTTTAAAAGAATTAAAACGGCAACAACTAGTGTTAGATTTAGCTAAAAAACCCGCTTATACCTGATTGCCCCAACCCTTAGCATTTCTGTTTGGTGGCTTAATGGCACTCAGGACAAGAAAGTACATTCGTTACCCTAAGAATTAAGATTAAGATAGATAAAGCATATGGCAGACATGATTGAAGCGAAAAGTTTACGCAGTGGACAAACGATTTTTGGTCCCAATAAAGAGATTCTGTTGGTGTTAGAAAACACCTTTAATAAGACCGCAATGCGCCAGGGAATTGTTAAAACCAAGGTCAAAAACTTACGCACTGGGGCCATTGTTTGGATTGAGTTTACCGGAGACAAGTTAGAACAAGTCATTATTGACAAGAAGAAAATGACCTTCTTGTACAAGGATGGTGCTAACTATGTTTTTATGGACCAACAAGACTACAGTCAAATTGAGATCCCCGAAAAGCAGTTAGAGTGGGAAAAGAACTTTATCACTGAAGACAGTGAAGTTACTATTATTAGCTACCAAAGTGAAATTTTGGGCGTTAACCTACCCGAATTAGTGCCGATTGAAGTGGAATTTGCCGAAGAAGCAGTGCAAGGTAATACCGCTAACATGGCGCGTAAACGGGCTCGGTTAGTTTCGGGTTACGAATTAGATGTGCCCCAGTTCATTCGTACTGGTGATAAGATTGTGATTTCCACCATTGACGGGAGCTATCGTGAACGGTACAACAAGTAAATTAACTAGAACCCAAAGACGGATTGCAATTGTGGAGTTTATCTTCGCCACGCTGTTCTTTTTACCGAAAACTGCTGACCAAATCCAAGCGGCCTTTTTGGACTATGATGTGCCAGAACGTCCGCTCAACGATTGGCAAAAAGAGATTGTCAAAGTCTTTAGTGAACGTTGTGTGGAATTCATTGAACTAATTGAAAACCAACAACAACGCAACCAAGCAGAAGTACAAAGCAAGTACAACAAGGTTTCCGGTAAAAAAGTCGACTTATTGACTAAGGCCGTGATTCTCTGTGCTTTGAGCGAACAACACGCGCAAGCGACTGATAAACCATTATTAATTAGTGAAGCCTTACTGATTATGGACCACTATTCCCAGGTACCCGAAAAGAAGCAAACCCATGCTTTATTGGATAAACTCTTGTAATGCGACGTAATTGAAGGGAACACTATAACGTGTTTGTAGCAAACCTAGCCTTAGTGTTGGGTTTTATGTTGAACATTGTGGTGGCGCGGTATACCTTAACTGGTGCTACGCCCCAAGCACGTTTTTTATTCCTCACACCCTTTTTAGGCATAGTAGCAGCGAGTATCTTCTACTTCTTTGATGTGAAGTGGTTCTTAGCGGATTATCCCTACAAGAAGTTCCATTTTCAAAAGAAGTGAACCTGGACTTACTTAAGTGGCGTATTTGTTTTCTTTGCTAATATCTTGGTTAATGTGATCTTGTTAGCACTCCTCGTTAACCAGATGACCAACCAAATCCTTAGTGAAAAGTATACGGGTTTACTGGACAACGCTTACCCACTACTTTGATCCGCTGTGGGTGTTAGTATCTTTTTGAGCTTAATTAGTATTGGTTTGAGCAAAACAGCCCACTTTAAAATTGATGTGGAAATGCTCAAAGCGAAAAAAGGCGAACCCACCGCCGCTGATAAAACCGACAGCCGGCCGGTAGTTGTTGATTTAGACCAAACTAAATCAAAAAAAGATGGGGATAACCCACCCCAAGCTTCGGGTGATATGACTAGTCTTTAGCTTCGTTACCGAACAAGCGGTGGTTAATTAACTCGTTTAAACTAGTGTCATCAAACAAGGTGCAGACCGCAGTAGTTGCAAATGCCAAAGCACAACCAGCACTACGACCAGTGACGAGTTTATTGTGGACCGTAACTGGGCTAACATTGCGGTGCTTTGATCACTTTAAGCTATGATTGGGAAACGAGGAGTAGTTTTCCGTTTCACTAATAATGCCGTGCTTTTACAACACGTTGGGGGTATCACAAATGGCAAAGACATACTTGTCATGATCCTTAAAGTAACGGATAGTGTCGAGTAACTGTTGGTCATGGTCTAATACCTTGGTAGCTCCCATACCGCCAGGGATAAAGACACCATCAAATTCCTCCAGATTAACCTTGCTAGTAATCTGGTTTACTAAATGTTACCCCATTACTGCCCTTAACTGTACTTAAGTTAGGGTGATAAATCACGGTTTGTAATTCTTTAACAAAACCAAATACCACCATTGTGTTGGTGTATTCCACATCATTCATGTCAGGGTAAACAATGACTAAGAGTTTTTTCATCTTTTATTTAGTACCAAATAAGCGATCCCCAGCATCACCAAGCCCGGGAATAATGTAATTATCCTTATTTAATTTTTCATCAATGGCTGCTAGGTAAATGTCCACATCAGGGTGCGCTGCTTCCACGGCTTTAATGCCCTCTGGTGCTGCTACTATGGCCACCACACTAATCTTAATCGGGTGGTTCTTTTTAATGGAACTAATGGCTGTGGCTAGGGTAGTACCAGTAGCTAGCATTGGGTCTAAGATAATCACGTGGGAGTTGCCAATGTTTTCGGGCATTTTCTTATAGTAAGAAATTACATTAGTGCCCTCATGTTCACGGTAGATCCCTAAGTGACCCACACGAATCTTTTCCGAATAGCGCACAATTGGGTCAACCATACCCAGGCCAGCGCGCATTATGGGGACTAGAACAATTTCGTTTTTGAGCTTATAACCCTTCGTTTTGGTAAACGGGGTTTTTACAACAATTTCTTCTAAGGGTGATTCCTTAGTTACTTCAAAAAAGAGTAGTGAAGTGATTTGGCTTAACGCCATCCGAAATTGCGAGGAGGAAGAAGATTTATCACGTAGCTTTGTCAATTCGTTTTCGATCAAAGCGTGGTGCACTTTTTTGATCACGGTTAAAGATAGTCTTAATAAGGTTATATTAAAGATTGACTAAAAAAGTTTTTTCTGGTTGTCTTGCTCCCTAAACTCATCGAGTAGTTGCATTTGGGTAAACGCTTCAATGTGTTTTTTGGTAATTTTAGTGCGTTTTTTAAAGTCATCGAGCGAACTAAAGGGCTTTTCGTTACGCGCTTCTATGATCGAATTGGCCACAGCTTCCCCCATCCCCGGAATCGTAATAAAAGGGGCAATTAGAATGCCGTTGTGCTCGACAAAACGAGTGGCGTGGGAGTGGGTTAAAGAGATTTGTTCTAGTTTAATGCCGCGGGCCATCATCTCTAAATAAACCTCGTAACTGGTTAACAGTTCTGCTTCTTTGGGTTTAATCCGTTTAATGCGGTACAGTGTGTTGAGCTCCTCTAGTTTTTGCTTGACAAAGCTCACACCTGATTTAAAACCACTGACATCATGTTCCTTGAGTTTAAAGCTCAACAAACAAGCATAGTAGCTCAACGGGTGGTACAGTTTGAACCAGGCAATTCGTCAGGCCATTAAAACATAAGCCGCGGCGTGGGCTTTCGGGAACAGGTAGCTAATCTTTAAACACGAATTAATCCAGTGTTGTTCGACCCCATTGCTCTGCATTAATTGCACTTCCTTGGCATTGAGAGCAATTCCCTTACGCACCTTTTCCATGATCTCAAAAGCATCTTTGGCCTGCATCCCCTTATGAATGAGGTAGAGCATAATGTCATCGCGACAGGCAATTACATCCTTTAAGCTTAAGTTTTGGTCCTTCAAGATTTTCTGCGCATTGTCCGCCCACACGTTCTTCCCGTGACTTAGACCCGAGACGCGAATGAGGTCACCAAAGCCCTTTGGTTTGGTTTGTTCCAAGATCTTGCGAACGAACTTAGTACCAAACTCGGGAATCCCAATCGCTCCCGTGGGTTCATCTAAGTAGTGCGGTTTTAGTTTTAAGGGATTGACCGACCAAAACATTTCGGTGAGCTTCTTGTCAAACCGGGGAATGTTTTGTGGATTGACATGGGTAAGATCGGCCAGGTGCTTTAACATAGTCGGATCATCCTGGCCCAGAATATCTAACTTTAAGATGGTGTCCCCTAACGCATCATATTCAAAGTGGGTCGTTTTTCAATCACTGGAAGTATCATCAGCTGGATAACCACACGGGGTAAACTCATATACCGATTTGTGGTTGGGGAAGATCATAATCCCCCCGGGGTGTTGGCCGGTCGTGCGTTTAATCCCGACTAACTTTTGCTTAAAGCGTTCAATTTCGGGAGCGGTGGCCAATTCGGTTTGTTTAATAATTTCAAAGTAGTTGCGCGCATACCCGTAAGCCGTTTTTTCGGCCACTGTCGCAATTGTGCCAGCACGGAAGGTGTACTGTTCACCAAACAGTTCCCTCACATAAGCATGGGCTTTGGCTTGGTACTCACTGGAAAAGTTTAAGTCAATATCGGGAATCTTGTCCCCAGCAAAACCCATAAAGGTAGCAAAGGGAATGTTATGACCATCACCCTTAAAGGCTGCTTTGGCACCACACTTTGGACAATCCCTTACCATGAGGTCATACCCATCATCGACCTCTTCGTTGACCTCAAAGTACTGACAGCTCTCACAGAGATAATGCGGCACTAAGGGATTAATTTCCGAGATACCAATCAGGTTAGCTACCAACGAAGAACCAATTGAACCCCGCGGTCCAACAAAGTAACCATCTTGAACCGATTGTTTGACTAGTAAATGGGAAATCCAGTAAATAATGCCAAAGCCATTGTCAATGATCGCTTTTAACTCTTCCTGGATCCGTTGTTCTATTAACTTAGGTAAGGGATCACCGTAAATGGCCTTGGCTTGACTCCAGGTTTTGGCTGCCAGGTTTTCGTTAGAACCTTCAATTACTGGTGGTTGGAGTTTGGTTTGCACTGGTACCAGGTCATCAGCGGTGAGTTGTTTCGCTAACTTATTGGTGTTTTTGACCACTAACTCGTAAGCTAATTGTTCGCCTAAAAAACTCATTTCATTGAGCATTTCCCCTGTGGTACGCACAAACACATTGGGAACCCGTTGTTCCCTTTCCTTATAGTTAAAGTGACGGTGTCAACGCCCACCCAGACCCTTGGCACAAACTATGGCCTTATAGTACTCATTCTCCCAGGGGTGAATAAAGTAAGCATCCGAGGCAACTGCCACTAACTTATTGAGTCTTTGGGCCGTGTCCACCACTAACTTAATTGCGTTAGGAATGGCTTCTTTTTTCAAACCTTCCCTGATGGTGTAACCAGCATAAGCATCAGGTGGGGCAATTAAAACAAAGTCAACTCTTTTAATGGCCGCTTCTAATTCTTTAAGCGGTTTAGTCAATGCCGCTTTAAAGATATCACCTTGCACCGGGTTATCAGTAATGACAAAGGATTTAGCAAACTTTTGGAGTGTTGAAGTTAACACTAGGGGTCGAGTAGCGTTGTTATCGGTGAGCGCAATTGATAACAGTTCATACAGTTTAGCAAACCCACTTTGGCTCTTAATGTAGATAATGCCGGTATTGGTAAAAGTGCGCTTGAGCAGTTCAAAGTGGCACTGCTGGTTTAACTCTTGGTCTAACTGCTCGAGATTAGTAATCCCCATGAGTTCGACCTGTTCCTTGAAGTAGTCAAAGACCTTCTTTAAGGCCTGGGTATCATAATCCGCTCGGTGCAATCGTTCATCATCAAAGTCAAACTTGAGCTTGGCACAAATATTGCTCAACGTATGGGAGGCAAACCCGGGATTTAAGGCCCAGGATAAGGCTAAGGTATCAATTAAGGGATTGGTTAGTGGCGCAATGTTGTATTTTTCAAACTGGGTTTGTAAAAAGGGCAAGTCAAAACTCAAACCGTTATGGGCTACCATTACACAACCATCTAGTCAAGCGCGCAGTTTTTCCAACCCCGCTTGCTGTTCCATCCCATCTTCTAGTTGTGCTTGGGTAATCTTGGTAATTTCGGTAACTGCTGCAGGTAAGGGTCCATCAATCTTGAGAAAGAACTGCATTCGTTCCACTTCACGGTTATGTTTGACCTTAATTCCCGCAAACTCAATCACCGAGTCATACCGACCGTGTAACCCAGTTGTTTCAATGTCGAAGATGACAAAGGTCGCATCCTTAAGTTTGGTTTTATCAGGATTGCTAACTATCTTAATGTGATCATCAATGAGGTTACATTCCAAACCATAAATGGCCTTCAAGCCGTACTTTTTGGCCACTTCATATAAAGTGGGATAAATGTGAATGTTGTCCTTGTCAGTGACAGCAATTGTCTTCCAATCGCGTTCTTTGGCAAACTGGGCGTATTCCTGGACACTGTTAATGCCATCAAAGGCACTCATCTTGGTGTGGAAAACTAATTCGACCCGTTTTTGTTTGTCCTGATCGGGGCGGCGATAGTAAGCGGGGGTTTCAATCGGGGAAATTTCCTTAATGATACCCTGGAGTAGTTGGGTCTTGGCATCACGTTCGACCTTAACCGTGGCCTTAACTCAGGTACCAATCTTAATGCCCTCAATTGTTTTGGGATCCTTGTAAAACCACTTCAAAATTAACGAACCACCTAGTTGGAAATCAGTGACATAAATATTAAGGGTATTACGGAGGTTGTTGTACCCAGGAAAGTCCTTTAACTCAAAGATTTGCCCCATTAGTTTGATCTCATCCATTTGTTGGTTAATCTTGTGAATGGGGACAAATTCCGTACTAAAGACCTCGGTTTCAAACTGCTTGTCGTACTTGGATACCTTCATTGATTTCACTTTTAGTTGTTTTTCCGCTGGTGGATTAAACAACGAGACAAAACCAAAGTGGGTAAACCCAGCTTGGTGCATCCACTTAATGAAGGCTTGGCGTTGTTCAATTAACCATTGGTCTAATTCTTGGGACTGGCAGTTAGCTTTCAGTTCATGGTTACTGTAGGACAAGAAGTTAGGATGGGTTAACTCTTGCTCTAACAGGATGCGGTACTTCTTGTCCTTACCAAAGAAGCTTTTGAAGTAGTCTTTGATAATGGCTAAGGTGACACTGGACTTAGTGGACCCGTCCTTAAAGAAGGTGGCATCTTCTTGGAAGGTAATCCTCAGTTCTGCCCCTTCCACATCCTGAAAGCCCTCATACAGGGCGTTTCAAATGTCAATTGTTAACGGTTTTTTTAAAGTGAGCGCCACGAGAAACAGGGCGTTTTCGCGATCATTCTCGAGGTAAACCAGTTCAATCCGGTTGTTGAGTTGGTGCAATTCGTTGTGATCGAGAATATTTTTCTTAACTAAAAACTGTGATAATGCTAAAATGCGTTTACCCTTTTCGGTTTCGGTGTCGAATACCATATGTGCTAGTTCAAAGCTTATAAAACTGAAAAGGTAACAGCTTTATCTAACTGTTACCCGACGGTATTAGTTTACTATCTTGATCGTTTTGAAAACTTAAATGGTTAGTTAACTAGGTTCATTTCAACCACTACCTTTTTCAAGTTCCCGATTTGGTGTTCAAACCTAAAAAAAGGTTTGCTAAACTTAGGTGAGATTGCAAGATCTCGTTTAAATTTCCTCTGTTGCTAGTGGTAGTGTACCCAACTTTAATGCCATAAACGATTGCTATTTATTGATGAAAGCGTTCCGAAAATTGTTGCTGGGTCTCGCCTTACCCACAACCATCGGTCCCTTATTAGGCTTATTATTAACTAATACTGATACTGTCAAAAATGAAAGCTTAACTACTGTGCGCCACAGGTCCAGCATTAACCAGGACTTTGATGGCATTTTTCATACCGTTAAGTTATTAGAACCAATCCAGCAAAGTAATGCTGATCCCGCCGCTAGTTTTGCGCTCTTTCAGCAAAAGTTTCCCAACCTAAAACGTGTAGCCAACTCCACCTTAAATACCTTTGATGTCTACAACCTTTTGAGTGGTTGGAAGAGTAGCTTAACAGCGTACCTCAACCAGGTGTTGGCACTCCAACAACGGATTAAAGCAGCTGATCAAATTTTCCCTAACCAAAAGGAAACTTTACCCAAGGATGAGAATCCCAACATCTTTGAAGTGTTAGGTGCATATGGTGGTAAGGGTTTCTTTCCCACGCTAGGATCCAACGGGTTACACCTGCCACCACAGCTGTTCCAGTTCTTCCGTGACTTCCAACTAAGTAGCTTTACCATTAAAGACTTTGAGGTAGCGTTAGTGAGTGAACCGGACATAGTGCAACATGACAAAGTGCGCTATGCCTTTCAGGTGCAATTTAACTTAGTGTTACAATTACAAATTAACCGTAACCCAGTGTTATTTGACTTTAACCTACAGTTAAAGACGAATAACTTTGCCAATCAAACCAGTTTTGATGAATTGTTTAACGAAAAAACTAATCCACTTAACTGGCAGTTCTTTAGCAAGCTTAAAGTTAACCATTTAGTGTATGAAGGCAATGACATAACTCAGTTAGCTAATACCTTATTACAAAGCCAGTTTAATGTCTTACAACTTGATTTAAATAAGTCAATTTACCGCTTGAACTTAAACGCAATGGCACAGCGCTTTGAACACGACTGGGTACAACCGTTGTATGCCAAACGCACGCAGGCCAAAATTGCTTACGAAGCGGAACAAGCACGGATTGCGGCCGAGAACAAACGCAAGGAACTAGAACGGCAAAAGCTGTTAGCAGAATTGAAAGCCAAAGCTGAGCATTACCAAAAGATCAAGCAAGCACGGGAAAACATGCTAAAGGGCTTAAAGAGTATTACTGATTTTGTCAAGTTCTGAAAGAGTCCGGACCGTTTATTAGTTGGCTTTAACAAACAGGACGACATTACTACTCGTGCTGGGGTTTACAAGGCGTTACAAATTGCTTACGCTAACTATCCGCAGTGAACCTTTTATCTAACCCTACAAGGTTGGAAGAATGGTTCGGAGTTGTTGTTAAAAAGACCTAGTTGAACTAACTTATTAAGTGATATCCACTTCCAAAAGGCCTTTAACTTAAAGAACACCGTATCAGAACAAACCCTGGGTGCAGCTACCCTACCAGGTTATGGTTACTATGGCTTACGCATGAGTGACTGGTTGCGCTGGGCTTTGGGCTATTACTCCTATATCCATATGGGCGTACCGCAAAATGTACAGACCAAGTTTACGGGCACCCCAGATAATGAACAACAAGTCTGGATTGCTAACGAACCGTTTGAATGGAACAAACATTACGGTGTTGGTCCTAAATACAAGGATAAGGCCTACCGCTTTAACATGGAAATTAGTTTTGAACTAGAGGGTTGAATTGCTGTTAAGTGGTGAGCATGAGCCTTTAAGGGTTCAATTCCTGGGAACTGAAAAGGCAAACTCAAAGTAACCCATCTGTTTGATGGTATGGTACCAGTGTGGGAATTGGGCCCAGTCAATACCCATTTACCCCAATACAGCTTTACCGATCAGCAACAACTGTTGTTTGTACCCCACTCCATTCAAAAGATTGAAGCGATTGGTGCGGACAAAGGTATAAACGATCTTTTAAAAACCCAAAACCTCCATAACCTAGAGCGCTTGTCATACGAATCAACTAACCCAATTGACCTCATTTCTTACCTCTTGTACGCGATCCAGTACATTAAGGTTTAGTCACTTTCAGCAGTTAAAGTTCAGGTGTAAAGTTAACGATTAAGATCAAAAACCGTTCCTAAAAAAAGATCTTTTTCTAAAATCTAAGCGAGTTACAACTCAATTTAAATTTTCTCTCTGGTTGGTTCGCCAACAGTTTTAGCCACTTCAACATTCGTCAAACACCATTAAATGAAAACACTGCACAAGTTGCTGTTGGGGCTTTCCTTACCAGCAATCCTCGGTCCGTTGTTTGGTCTACTAGTTACAAAAACTGATGCTGAATCACCAGTTGCAGCATTACATAAATCGAAATCTTTTAGCAAAAACTTAGATAGTTTCCGCGCCTTATTTGTGGGCGAGGATATTGAGGAACAACTAGCTAAGGTACAACAACAAACACCAGAAGTTAGCTTTGCTAGCTTTCAACAAAAGTTTCCTGATAAGGCCAGTTTAAGAAACGGTTTTCAACCAATTGATGTTTACAACTTTTTAAGTGGTTGAAAAGGTGCTTTAGAAAACTTCTTGGCTAAGGTAGTTGAACTGCAAAAGAAGATTAAAGCAGCTGACGATATTTTCCCGAACCAAAAGAAAAATCCCGACAAGAAAGATAACCCAAATGTTTTAGAAGTGTTAGGTGAATACGGTGGAGATGGTTTCTTTCCTACATTAGGTAATAACGGTTTAAACATCCCGGACCAAGTTTTCCAATCCTTTAATAACTTCCAAATTGAGAACTATAAGATTAGTGACTTTAAGGTGGACATTGCCAGTGAACGTGACATTGTCCAACACGACAAGTTCCGTTTTAGTTATGTCGTTAACATTGGTTTAGAGTTAGCACTTTTAGTCAATAAGCGTCCGGTTTACTTTAACTTTAGCTTGGATCTGCGGACCAATAACTTCTCCAACCAAGCCGGTTTTAACGAAATCTTTAATGCGCCTGGTAACGCAGCTACTAACTGACAGTTCTTCAGCAAGATTAAAGTGAAACAGTTGAACTATGATGGTAATGACAGTACCCATTTAGCCAATACCCTCTTACAAGATCAGTTCAATACCTTAAACTTAAACTTGCAAAAGTCGATCTATGAGTTGCCATTAACCGAAATGGAAACTAAGTTCCAGCAAGAATATGTGGAACTTTTACTAGCTAAACGTAGAGAGGAAAAACGACTTTGGGATGAAGAACAAAAACGAATTGAAGCAGAACGGAAACAAAAAGAAGCAGAGCTAGCACAAATTCAGCGTGAACTTAAAGAAAAGGCTGAGAAGGATGCGGCGGTTAAACAAGCTCAAACTAACTTAAAAACTGCTCTAAGTAGTGTGGAAGGCTTTGTGAAATTCTGAACTGAAGGTGAAGACCGCATTAAGTTGGGTTTCACGAAGGAGGACAACCTTTATACCCGTGCAGGTTTAGTGAAAGCACTCAAAATTTCGTTTGCTAACTACCGCGCTTGAACCTTCTACATTACCTTGCTTGGTTGAAAAGCAGGTAGTGAAAAGTTACTTCGGAAGACTAGCTGAACTAACCTATTAAGTGACGTTAACTTCCAAAATGCTTTTGGCTTAAAAAATACAGCATCGGAAGAACAAGTTGGTAAAACATCATTACCAGGTTATGGTTACTATGGAATTCGCATGAGTAACTGACTCCGTTGGGCATTAGGTTACTATGCTAATACCCATGCGGGTAGTCCACAAAATGTGAAAGCCACCATTGATGGTAATCCAAGTGATACCACCAAAGTATGGATTGCACCAGAAGACTTTAAACAGGGTGTACACATTGGTGTGGGGGAACACTTCAAAGGTAAGGCTTACAAGTTTAAGTTTGAAGTTAGTTTTGAATTAGAAGGCTCGATTGCAGCCCACTGGTGAACTTTAGCCTTACGTGGTAACATCCCGGGCTACTGAAAGGGTAAACTCCAAGTAACCCACACTTTTGATGGTGATGTACCTTCCTGGTACTACGGTTCGGTACAAACCCACGCTCCTGAATACCGTTTTACTGAAGATAACAAGCTATTATTTGTACCGCACTCCATTCAAAAGATTACCGCTGTTGGTGGTGATAGTAATGGTGTCAACGGCTTGTTGAAATCACAAAACTTACATAATCTCGAACGTCAGTCTTATGAGGTTACAGCACCAATTGATCTAGTTTCCTATTTGATGTTTGCGATTGCTAAAGAACCAACAAATAACTGGTAGTTCTTTGCTGTATATAATTAGCGCTTTATCTAGCACTATTTAGATTTCAAAAAAAGTCTAAGTGTGAATAAAAATACTAACAGCAAAACCTAATCAATATAGGCACTGTTGGTATTTTTATTGTTATTTGGAATTATTTAGATTCCAAATTGTCTAGACGTCTGTTTATGCCTTCAAGTGCTTTCAAAATTAATCTAAGTGTTTCTCCTTGATTTTTTTGTTCAACTTGAAGCTCTTTAATTTGTTGGCCTTGAACGATAACAACTTCAGTTAGTCGATCAACTTTGTCCTCAACAACTTGAATCCTTTGACCCTGACTCTTTAACTCAGTTTCAATCTTTGTAAGTCGTTGGTCTTTTGATTTTTTGTACTCGTTAAACTCTTTACGCGTTACGTATTCAGTGTTCACTGGTATAACCGGTTTGTTAAGTTTAAATTGTCTGTTGAAATTGCCAGAGAATTCCACACTGTTGTTGTCTTTGTCGATAATATATCAACCTGAAAAAGTACCCTTTTGGTCTTTTTCACCATGTCATGAAATTCTTTTTCGTTATAAAACGGAATTTTCTCTTTCATTAAGCCATACCTCCTTTCTTTTCAGTGAAATTTAAAAAGTAACTACTAATTGCAACTTTGTTGTCAACGAGGATCAACTGCTTGATTGATCAATATGCAAAATTATTTCTGTGCATAATATTGTCTCCACTAAATAAATTGACCAGACTTTAAAAAGGTGCCAAAAATTAAATCAAAAAGTTTTTAAGGATTTTTAAAGGAGTAAAAGTACCAGCAGCTAACCAATAAAGATTAACTGCTGGTACTTTTACTTTGTGCTTTATCTACTTAGATTCCAATTTGTCTAAACGTTTGTTTATACCCTGGAGAGCTTCGAGTATTAATTGAAGTGTCTTGCCTTGAGCCTTTTGCTCAATTTGAAGTTCTTTGATTTGCTTGCCCTGAGCTCTGAATTCTTCCTTAGTTACAAATTTTTCACTAAGTTCAACTAGAAGATCTTGTTTGAACACTTCTAGTTTTTTATCCAGTTGTTTATGGGTGACGTATCCACTTTTACCTTTTTCCATATGGTTTAAATTGCCATGAAATCCCATGTCGTAGCTACCATCAGGTAGTTCTTCTTCCCATGTATAACAATCATTAATCTTATCTTTATTTAGTCTTTTCTTAAACAAAGTAAGGGAAATTTTGCGTTTAATGGCAAAGACCTCCTTTATTTATTGATTGGAAAAAGGTGAAAGTGTTTAAAGAGGTAGGCACCAACAGGTCACTGAGCGAGTTGACTGCCCAAATAAAATAAATGATTTTTTAAAACTGTCATTTTATTACGCATACATCATTTAAATTACCCAAAAAACAAAAAGACGCCAAAAATTAGTGTGCAAATTTTACGAGTTTAAAAGTGTCTTTTTTAACTGGTAGAACTTTGTCTCAAAAATTAACAACAGTTAAACCGGCAAAAATAAAAATTTTGGGTTAAACTTTGGGGCGAGGTTTCCTCAGTTAAATATCCTTTTGGTTGGTTTTGCCAACAGTTTTAGTTGTCAGATTTTTTATTTGAGATTGCTAATAATTAATGACAAAACTCCCTAAATTGTTACTGGGGTTAACCTTTTCTGTCTCACTTATACCGTTTTCTAGTTTGCTTATTACTAGTACTGATGTCAATAAGTAACCGGTTCCAACTGCCTTACAACGCACTGGCGCCTCTGCCATTCATGAAGGTAGTTTTCAAACTATTACTTTAGGTCAAAGTTTAATGGAGCAAATTGAACAGCTCCAACAATTTACGCCAGCGCAGCGCTTTACCCAATTTAAAAAGAAATTTCCCAACCAAAAATTACTCAGTCAAAGTGAGTTAAGTCCAGTTGATGTTTACAATTTTTTAAGTGGTTGACAAGGTGCTTTGGTCTCTTTCTTGGATAGAGTAATTAAATTACAAGGCAAGGTTAAGGAAGCAAATGAAATTTTTAATCCAAATGTAGGTGATCAAATAGTTTTACCTAAAAAGGAAAACCCGAATGTTTTGGAAGTATTGGGCGAGTATAACGGTTTTGGTTTCTTCCCTACATTGGGTAAAAACGGATTAAACCTACCACAGCAAATCTTTGAAAACTTTACTGACTTTAAAGTAGAGAGTTACCAAATTAACGATTTCAAGGTAAGTCTAGTGGGCGAGCGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAACTGCTTGTTAACAACCAAAAGGTTACTTTCAATATTACGGTTGATTTGAGAACCAATAACTTTTCTACCCAAGAGACTTTCAATGATTTATTCAACAATGGAACTGCACCAACCAACTGACAGTTTTTTAGTCGAATAAAGGTAAATAAGTTGCACTATGACCAAACTGATGCCACCCATTTAGCTAACACCCTACTACAAGACCAGTTTAATGCGTTAAACCTTGATTTACAAAAATCGATTTACGACCTTAACCTTGATGGTTTAGAAGAAAGGTTTGAAGAGGAATACGCTAAGCCACTTCGTGAAAAAAGAACTCAGCAAAAGAAAGAATGGGAAGAGGAATAGCGAAGACGGGCAGAGGAAGATGCTAAACTGCAAAAAGAAATTGAAGAACAACAAAAGAAACAAAGAGAACAAGCAAAAAACGATGCTGAGCTCAAAAAAGCACAAGATGATCTCAAAGTTGCGCTTAATAGTCTTGACGGTTTTGTTAAATTCTTTACATCAAACGAAGGTCGCTTTAAATTAGGTTTTGAAAAGACAGATGACATTAAAACCCGTGCAGGGTTATACAAGGCACTGAAAGTTTCTTTTAGTAACTACCGTGCTTGAACCTTCTATCTAACTTTATTAGGTTGGAAGGAAACTACCGCAAAGATACTTACAAAAGCTAATTGAAAAGATATAAGAAGTGACGCTAATTTACGCAAGGCCTTCGGTTTAAAAGAAAGTGCCAACGAAAAAGAGGCTTCCAGGGTCATTACTCCGGGTTATGGTTACCAAGGTATTCACTTAAAACAATCGTTGCGTGAGGCGATTGATAAATACGCTACTTACAGCGTTTCTCGACCAAATAACGTCACAACTTATTTACCTGGCAAAGTTGATGGAAGTGGAAAAGATCAAATTTGAATTTCGCCAAACAACTTTCAAGTGAATCGGGAATGGGGTAATGGTGCACATTTTAAAGATAAAGCATATCGCTTTAATTTTGATGTCAAAGTTGAATATGATGTAGAGGTAAAAGCTGCTTGATGAACTGCTCTTTTCCGAGGTAGCATCCCAGGGTATTGAAAAGGTAAATTTAAAGTTACTTACTCCTTTAATGTTGAAGTACCTTCTTGAAACTATGGTGACAAGCAAGTGCGTCCACCACAATACAGTTTTAAGGAACAAGAAAAACAACTTTTATTTGTACCACGTCATGTACAAAAAATTGAAGCTGAAGGTAAACACCTTGAAATAATTAATCCGTTCTTAAAAGATCAACATTTAGATTTTTTTGAACACTACCACCCAGATTTAACGCAGCCTTTAGACATGGTGTCATACCTAATGTATGCCATCGCTGACAAGGTGAAATAACAAAAACATTTTGTCTGACAGTTGTAGCGACTCTTATCCATTTCTCAATTAATTATTAGTAATGAAAAAACTCCACAAAATTTTATTGGGTTTATCTTTGCCCGCATCTTTGGCGCCCTTATCTGGGTTTATTAGTACTGATGCTGATAACTCACAGTTTTCACTAAAGAAGTCTAGCACTGAAATTAAAGGTACACAATCCATTCACCTGCTTAATTTAGGGGAAAGTTTAACTGAAGAAATTAAAGAGGCACAAAAGCGTACCCCAGAAACTAGTTTTGCCAGTTTCAAACAAAAGTTTCCCAATAAGGAAAGTTTTGTTAAAGGTTTTCAACCAATTGATGTCTACAACTTACTAAGTGGTTGAAAGGATGCCATTTCATCCTTTTTAGATAAGGTTGTTGAGCTACAGAAAAAGATCGAAGAAGCTAATAAAATTTTCAACACTAATATTGGCAACCAAATTGATTTACCCGAAGATGAAAATCCCAATGTGCTAAACGTTTTGGGCTCATATGGTGGTGAAGGGTTCTTTCCTGCATTGGGTAAAAACGGGTTGAACCTACCACAACAAATCTTTGAAAACTTCACTGATTTCAAAGTTGTTAGTCATAAAATTCATGACTTCCAAGTAAGTTTGGTAGGCGAACGTGACATTATTAAAAACGACAAAGTCCGTTTTTCTTACGCGGTGCAAATACCACTCAACTTAGAGCTGCTTGTTAACAACCAAAAAGTTACTTTCAACATTACGGTTGATTTGAGAACCAATAATTTCTCTACCCAAGAAACTTTTAACGAGCTGTTCAATAAATGCATTGGCCCCGTTAACTGGCAATTCTTTAGTAGGGTTAAAGTAGATAAATTGCACTACGATCAAACTGACGCTACTCATTTAGCTAACACGTTGTTACAAGACCAGTTCAATGCGTTAAATCTTGATTTAGAAAAGTCCATCTATGACTTGGAACTACCTAGATTAGAAGCAGAGTTCCAACAAAAATACGTTGATCCTTTAATTGAGAAAAAGCAAAGACAAAAAGCAGAGTGGGAAGAAGCGGAACGTATCCGTAAGGAAGAAGAGGAAAAACACCAAAAGGAGCTTGAAGAACAACAAAGAATTCAAGCAGAAAAAGCGAAAAATGACGAACAGTTACGTAAAGCGCAAACTGAATTGAAGAAGGCGCTTGGTGGCATCGACTCCTTTGTTGAATTCTTTACGAATAATGATTTACGTCTTAAACTAGGTTACACCAAAGAAGACAACGTTAGAACCTGCGCTGGACTTTTTAGAGCACTAGAAGTTTCCTTTGGTAATTACCGCGCTTGAACCTTCTACATTACATTATTAGGTTGAAAGGATACTACTGAAAAGATCTTTAAGAAAGCTAAATGGCAAGACATTAGGGATGATGAAAAATTCCGTAAGGCCTTTGGTTTAAGTCCAAAAGCCACCGAAAAAGATGTTGGTAAAGTAACCAATCCAGGTTATGGTTATCAAGGGATTTACATAAAGGATTCCCTGCGTGATGGAATTGCTAAGTACTCTGATTCGACGGTGTCTGAACCTAAAAACGTTAAGGTTTCTTTACCAGGTACTGTTGGTGATAATGAAGAGGGTAAGATCTGAATTGCTTCCCATAACTTTAGACAAAACCACGAATGGGGTGCTGGTGAAAAATTTAAATATTCAGCTTATCGTTTTAAGTTTGATGTAACAGTGGATTATGATGTAGAGGTAAGTGCTAAATGATGAACTTGAGCCTTTCGGGGTAGTATTCCAGGATATTGGAGGGGTAAATTCAAGGTTACCTATTCCTTTGATGGTGTCGTACCTTCTTGGAAGTATGGTCACATCCAAGTGCGTACGCCACAATACAGCTTTAATAAGCAGGAACAAAAGATTCTGTTTGTACCCCATGCTATCCAAAAGATTGCTGCTGAAGGTAGCAACTTAGATTTAATTAATCCATTCTTAAAGGATCAAAAATTAGATGAATTTGAGCACTACCACCCAGATTTAACTAAACCATTAGATTTAGTAGCTTATCTGTTATACGCAATAACCACGAGATCATAGTTGCCTAGTGGGATAATTAATAGAAATAAGAGCTTCTAGGCTTTTTTCATTTATTACAGCTTTTTCTTGGTTGATAAATTTACAAACAACTGCATTTGCCCATTTTACAAAAGTGCTAAATCAAGGGCTTTATTTGAAAATTGACCCGTTAAAAAGTTAATTAATGGGAGGATAAGCAAGCTTTAAAAAGCGCAAAATAGCGTTTAAAAAACACCTACTGTTTTCAGTTTTATTTTGAAAAATTCTAAATAAGAGATATAATTTTACTGATAGCTTTTCTTAAAAAATAACTTTTTGAGAGGGCGAAAATTCTTTTTACTAATATGTTTAATTTAAGTGATTTTAGTGAATTACCACGTTGGATTGGTGCCGAATTTTTAGGCACATTCTTTTTAATTCTTTTAGGTAACGGTGCTGGTTCGCAGCTAACACTCAATAAGATGTTTGTTAAAGAATCGAAAGCAAAGTTACTAACTGCTGCTTTTGCTTGAGGTATTGCTGTATTAGTTGGTGTTTTAATAGCTAACTCTTTGTTTGAGGGTGCGGGTAATATTAACCCGGCAGTGAGTTTGTTCTATGCTGTTTCGGGAACTATTCAAAAAGCTTTATATCCATTGCATGTAAACTTCAGCATTCCATTACTTTGAGTAGCACTCCTACTTGCTTGAGTAGCACAGTTTGCTGGGGCAATGTTGGCACAAGCATTGTTAAACTTCTTGTTCTGAAAGCATATTGAACAAACTGATCCACAATCGGTTTTGGTTACCCATTGTACTAACCCAGCAATCTTTAACATTCCTCGCAACTTTGCAACTGAGTTTGTAGCAACTAGCGTTTTAATTGCTAGTTTACTGGTGGCTGGTAGTTTTGGTGCCAACCGATTTGACCAGTCACCACGAGGGGTTGTACCAATGTTAGTGGTTACTGGCTTAATTATGTCTTTTGGTGCCGCTACTGGTACTGCAATTAACCCAGCCCGTGATTTAGGTCCGAGAATTGTTTACTGATTGAGTCCAATTAAAAATAAGGATCCTAACTTAAAGTACAGTTGGATTCCGGTAGCTGCACCTCTAAGTGCTAGTGTTATCTTAGGTGTGCTTGTGGCTGTTATCGTTTAGTTCCTTAAAGCGTTCTTTAAACTGCTTGGTCTTAACTGTAACTGGCTTACCCTGCACGTGGTGGTGCAACTTACAACGTGGTTCATAAGCTTCAATATCACCAATTAAAACGGTTTCGTTGCTCTTGGACACTATGCGCTGGGTGCGTTGGGCTAACTGACCACAGACATTACAAACGGCATCTAACTTACAAATCTTGTCGGCAATCGCTAACAGTTGGGGAATGCTACCAAAGGGTTCAGCGCGGAAGTCCGTGTCTAAACCAGAAACAATGACGTTAATGCCGAGATCATTGAGTGATTTAACCACTTCGACAATTTCACTCGAAAAGAATTGGGCTTCATCAATGGCAACCACATCAAAACGATCTTTAGTGAGGTAGTCATAGATTTCTAAAGGTGAATTAATTTCAATGGCTTCATCACTGTGACCGTTACGGGATTTCACGAGGTGTTGTTGGCGCGTATCAATCTTTGGTTTAAAGATAATAACGGGAATCTTGGCGAGCTTTCAGCGCTTAATTTTGCGCAATAATTCTTCGGTTTTACCGGAAAACATCGGCCCACAAATCACTTCGATTCAACCACGTGGGGATTGGTGGAAAACTTGGGAAAAGCTCATAGTTGCCCAATTATAATTAAACAAAGATGAGTGTGTTGCAAAAACCGCGCGGGGTTAAAGACTGGTATGGTGAGGAGTTAATCTACTTCAATTGAACGGTGCACCAGATCACTAATTTGGCCTGAAAATGAGGCTTTAGTGAGGTCAAAACACCCCTGTTGGAATACGCTGAAGCCTTTAAGCGCACCAATGCCAACGCCGATATTGTCAAGAAGGAGTTGTATGAATTCCATGACAAATCGAACCGGCTGTTAGCCCTGCGTCCCGAGGCTACTGCTGGGATAGTGCGCTTGGTCTGTGAGAACAAACTACTCCAACCACAAAACTACCCGTTAAGGTTGTTTACCATTGGCACCATGTACCGGTATGAACGCCCCCAAAGTAACCGTTACCGCGAGCACTACCAGTTTAGTTGTGAAGTGATAGGTGACACTAATCCAACCGTGTTACTCGACACTTTACTGTTGGGTCATGCCATTATCCAGCAATTGGGAATTGAAGGAGTGATCCTCAAACTTAACAACTTAGGTAACAGTGCTACCATTCAACAATGAAACCAAGCGCTGCAAGCTTACTTAACCCAGTTTAAGGCGCAGTTAACCGAGTTATCACAAAGCCGTTTATCGACCAATCCTCTAAGGATCTTGGACGACAAGGTTGATGGTCAACTACCCTTTATCAGTGATGCCCCCCAAATTGAGCAGTTCTTGGATGCTGAGCAGCAGGCACTGAATACTTGACTGCAGCAACAGTTAACCCAACAACAGGTGCCGTTTGAGTGGAATCCAACCCTAGTCCGGGGCTTGGACTACTATACTGGGGTAGTGTTTGAATTTGTTAAGGATGACACCACGGTACTAGCTGGTGGGGTGTATGATAACCTAGTGGAAGAGTTAGGTGGTACACCTACTAAAGCGCTGGGTTTTGCTTGTGGCATTGAACGCTCAATTAATTGTTTAAGTGCGGTTAAAAAGCAAGCAATTTTGGCCAATCAACCCCCACGATTACTGGTTATAGGTCTCACCGAAGCAGCGTTGGAAAAGCTGTTACAACTTTCATTGGGTTGAAGGGCGTATCATCCTGTGACTATTTATCCTAAAGTTATTAGAATTATCAATGGCATCAGAGCCGCGCAACGGTTAGGTTACCGCTTTTTAGGGGTCATTGGTGGTAATAATTTAGAACAGCAAACCATTACCGTTAAAGACCTGGCTACTGAACAGCAAACAACATACACTTGAGATGAATTTAGACAACGCCAAGTGCTTTAAGCAACGGGTTTTTATTGGCAATTTAACTTCCGAGCACCTCAATAAAACCGTTACTATTGCCGGATGGGTCAAACGGATTAAGAAGTTAGGTGAACTTAACTTTGTTATTGTCGGGGACAAGACTAATACTATTCAGGTTACCTGTAAAAATAAGGAACAGGTCAAGTACTTAACCAGGGAAGACTTGGTGATTGTCAAGGGCAAACTGAAACGCTTAGACAGTGTTCGGTTTGAAATTACCAATCCTACTATTACCTTATTTGCCAAGTCCAAAACACCCCCTCTTATCATTGAGGACAAAACTGATGCTTTAGAAGAAGTGCGTTTGCGTTACCGCTACTTAGACTTACGCCGTCCGGTGATGCAAAAGCGCTTGGCGTTACGCCATAAGGTAACTTTAGCCGTCCGGAACTGACTAGATCAAATGGGGTTTATTGAAGTAGAAACACCTACGCTCACGAAGTCCACACCGGAGGGTGCTAGGGACTTTTTGGTACCGGCCAGAATTAGGGAACATAGCTTTTACGCTTTGCCCCAAAGTCCACAAATTTATAAACAGTTATTAATGGTTGGTGGTACGGAAAAGTACTTCCAAATTGCCCATGTTTACCGCGATGAAGACAGCCGCAAAGACCGTCAACCTGAACACACCCAAATTGACTTAGAGGTGGCCTTTTACACCAAGGAAATGGTGATGGATTTAATCCAAAGGTTATTTGTCGATGTCTTTCGCCAAGTGTTAAACATTAAGCTCAAAAAACCGTTTCCAGTGTTAAAGTTTGCTGAAGCCTTTAACCGTTTTGGCAGTGACAAACCGGATTTACGTTATGGTTTCGAACTGGAAGACTGCACTGATTTATTCCAAGACAGTCCGAACCAATTCACTAACTTAATTAATGCTGGCGGCATAGTCGGTGGGATCCAATTACCGAATCTTTATTTAGACGAAGTTTCTTTTAAAGCATTGAGGAAACTAGCTAAGGACAACGGGGTTTCCTTAGAGTTTTACTCTGATAAAGCTAGTTCCCTAAAACAACCCCTGGATTTACCGTTAGCAGGCACCATCTTACTGGTAGCACACAAAAGTAAAACGCAAGCTTGAACGGCATTAGGCGCCATTCGCAATGAGTTGAAGTACCACCTCAACTTAGTGAAACCCAACCAGTACAGCTTTTGTTGAATTGTAGACTTTCCTTTATACGAGTTTGACGAAAAGGAACAGAAGTGGGTTTCGGCGCACAACATGTTCTCCAATCCCCAACCGCAGTGGTTGGTTAACTTTGAAAACCACAAGGCTGAGGCGTTGAGTGAACAGTACGACCTAGTTTTAAATGGGTTTGAACTGGGTAGTGGTTCAATTCGGATCCATGACCCAGAAGTACAAACCCGTTTAATGCAATCTTTAGGGGTGGATCCCCAACAGTTTGGCTTTGTGATGGAGGCTTACCAGTACGGTGCTCCTGTACACGCTGGCATGGGTTTGGGATTAGACCGCTTAATGATGATCATTAACAATGTGGACAACATTAGGGAAGTGATGGCCTTTCCGAAAAATGCCCAAGGGATAGAGATGCACACTAATGCCCCTGATCAGGTTGACATTAAAGACATAACAACAATATGGTCCAAACACCCAGTGAAATAAATACCCACCTTAAACACCTCTTGGCCTGTGATGCTTATAAGCTATCACACCGCTTAATGTATCCTAATGACACGACCAACTTATACAGTTGTTTAACAGCCCGGGGTGGTCGTGGTGGTTTTCCAAATTTTGTGTGAAACCACGAGTTTGCCAAAAAGATTATTCTTGAAGTGTTTGGTAACTTTTGTGATAGTGTGTTAGCAGTCCAAAACGATCCTGGGTTGGCCCAAGCGCTCACTGACAAGGTGACCACTGTTTTTGGTGATCCCCAGTTTGGGTTGGAGTTTACCCAGCACATCTGTTATTTAGCTAACTTTTTAAAACAACACCACCAGTTACCCTTAACGGTAAAGATTCACCAGAGTTCTGAGGGTTTAGCCTTCCGTACACCCTTAGTAACTATTACAGGTTCGGACCAAATGGTTCCCGAGTTAGTTTGGTTAGTCAACTATTTTGAAACCGTATTATTGGAAAACATCTGATTGTACCAAACTACTTTAACTGTCGCCCAAAGCTTAAAGCTGCTGTTGGAACGCTATGCTAACGAAACCGCGGATAATACTGAATTTACCCACTTTCAGTGTCATGATTTTAGTATGCGTGGTATGAGTAGTCTTCAAAGTGCTTTATATGTAGCTAATGCTCACCTGCAGTACTTTAGTGGCAGTGACACCATTTTAGGCGGTGTAGCTGCTAAATCGATCTTAGCTAGTGAACACTCGGTGATGTGTGCTGATGGTCAAGAGGGTGAATTGAATACCTTTAAACGTTTATTGGAGCAGTTTCCTAACAAAAATCTGTCCTTAGTGATTGATTCTTATGACATGTGGCATGTTCTAGATAACATCTTACCCCAACTCAAGGACTTAGTTTTACAACGACAGGAGAAGCTGTACCTCCGACCTGACTCGGGCAACTTTGAAACCCTTATTTGTCAGGGCAAACGGTTTAACCCCGAGGACAAAACTACCTGGGGAGTAATTGATTACTTAGATTACCACTTTGGTTCCACTGTTAACCAAAAAGGTTATAAGGTTTTGAACCAAAAGTTGGGTATTGTTTATGGCGATGGCATTACCTATGAGCGGATTGAGTACATTTTGGAACAGCTCAAACAACGTGGGTTTTGTTCTTCCAACATTGTCTTTGGGGTTGGTAGTACAACTTACCAAAACCTTAACCGTGATACGTTGGGATTTGTGTATAAGTTGACAGCCATCAAAAAGGGTAATACCTGACATGATGTTACCAAGAGTCCCATAACCGATCCCACCAAGCAGTCCATAGGGGGACGATTTGATAACCCCAACCTAATTCAGGTTTACGGTTAATGCACCTCAAAAAACCGTTAGTGGTTACTAAGGATTGCTTTTATTGACTTAGTTTCGGATCGCTAAAATGATTGTTAGCACCAATCTTTTTAAAATTTTCCAAACATTAAATTTTCTTTGTTGGTTGTAGTTGTTAGGTTGTTTAGTCAAAGATCCACCTTTTCGTAATAGTTAATTAAGTAATTTAGTTAGTTTCTTTTTATCCAATTTTAAACAGACTAAAAAAATCCGCTTTCGGTATAAAGCGTTTCTACTAACTTTATTTGCGTCAACCACAACGCTTACTGGATTCATAATACCTACTTTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGCATTGCGCAACAACAGTAGTTTAAGCCGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGCCTTTGCTAAACAAAAGGATTGAAACCAGAACTTTGTTAAATTTCAAAAGCAGTTTGAAAACAAGCTTTTAGACCCAAAGAACTTTAGCTTAACCGATGTTTATAACCTCTTTAGTGGTTTTCAACAAAGTGTTACGGCTACTGTTCAGCTAATGAACGAGTTGCAAACCAAAGTCAATGAGGCTAATAATATTTTCCCAGTTGAAGCCTTCAAGGTACCTAAGGTTCCAGAAAAACTGTTTGGTTTTGTCAACCAAGGCTTCTTTCCTAAACTAAACCCTAAGGGCTTAAACATTGCTGATAACGTGGCTTCCTTATTTGAACAATACAGCTTAAAGCAAGCAAGTCTTAAGGACTTTGACATATTATTGGAAAAGAAAAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTAACTTTGAAACCACTTATGTTGGTACTGGTGGGGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACTACTAACTTTAGTAGCTTAGAAGAACTCCAAGCTAGCTTTAGTAAAACAGGCGATAACCTTACTGCCCAATTGTTCTGAAAACCAACTGTAACTAAGTTAGTTTCTGGGGAAAATGATCTAACCCATATTGCCCAAACAGCAATCGGTGAAAGTCTGTTTGACAGCCGGGTTGACTTAAGTGCTTCTATTATTAATAGTGAAGCAACCCTTAAAACAGCCGAAGCAACATTTACAACCCAAGTTTTAAATCCGTTTAAAGCGGAACGGGAAAAGGCTTTGGCCATTAAGAAAGCTGAAGAAGAGAAAATTAAAAAAGAACTTGAAGAGCAAAAGAAGCGTCAAGAAGAATTAGCAAAACAACAACGAGACAAAGAGGCATTACAAAAGTCACTTTGAAAATTTCAAGAATTTATTAGCTACTGAAACGGTCAAGGAAAAGACGTTAAGCAAAAAGAACAATTTATTCAAGCATTAGAAGCGGCGTTTTCTACCAATTGGAACGAGGTTTTCAATTTGTTAATTGCCGGTTTTAGAAGTGCAATTCAGACTTATTACAAAGATGGCAAAGCAGATCAAAGCCAAAACGCTAAAATTGCTTTTGGCGAAAAGGGTATTCAATTCCCTAAATCTGGCCCAGGACTTGACGGTATTTTCATGTCAGATTTCCTTCGTGGTAACCTTACCGGCAACGCTCACTTTGATTTAAAACTTAAAAAAGTTGAAGTTAAAAACACACAAGGAAAAGACGCACAAGGCAATGATAAAAAAGCCTCCATTAATTGACAAGCTAAGCAAAATAACTTCCCCTTCAGACAAGTAAACCCATGGGATTTTTCCTTTGAAGTAGAACTTAAGTATGAAGGTAGCTATGGCCTATATCCAGGTGCTAGGTTCCTTAACTTATTTGGTTCCTTAGGTATTCCTAACGATTGAAAAGGCGAAATGAGCGTTAAGTTTGTGCTCGATGGTAAGACACCTCAATGGATTGCTGATAAACCAGATTACCCAGGTTCCTTGTTTAAATTTGAAAAGAACCAGTTAAAGTTCACTCCCCACGTTAAGGAGCATGTACATGTTGAAAACAAACAATTTATGGAAAAGTTGAAAGAGTCAAAACTTGCATAACTTGGAGTTAGAAACTGGTGCTACAAAACCACCTGTTGTGGACTTAGCTAGTTATCTACACTACCTCATTTTGAACCACAAGTAGATTTAACGTATTGCTAAATTGGTTGTATAAGCCAAACTTTATACAATCTTTCAAACATTAAATTTTCTTTGTTGGTTGTAGTTGTTAGGTTGTTTAGTCGAAGATCCACCTTTTCGTAAAAGTTAATTAAGTCATTTAGTTAGTTTCTTTTTATCCAACTTGAAACAGACTAAAAAATGCGCTTTCGGTATAAGGCGTTTCTACTAACTTTATTTGCGTCAACCACAACGCTTACTGGATTCATAATACCTACTTTGAGCCAACACGGTTCGAGTACGGATCCGAGTTTTGTGGCATTGCGCAACAACAGTAGTTTAAGCTGTGATGGTAAAAGTCGGCTTTTAGAATTTGGTAATGACCTTGGTTTTAGCAAGGAAGAAACTGATGTGTTAGCGAAACAGAAAAACTGGGAGCAGAATTACACTAACTTTAAAAAGCAGTTTGAACACAAGCTTTTAGATCCGAAGAGCTTTAGCTTAACTGATGTGTATAACCTATTTAGTGGTTTTCAACAAAGTGTAGCTGACACGGTTAAGCTAATGAATGAATTGCAAACTCAGGTAAATAAAGCTAACGACATTTACCCAGTGGAAAGCTTTCAAGTGCCTAAGGTACCACAAAAGCTATTTGGATTTGTCGACCAGGGCTTCTTTCCGAAGTTAAACCCGAAGGGCTTGAACATTGCTGATAACGTGGCTTACTTGTTTGAGAAGTACAGCCTAAAACAAGCAACCCTTAAAGACTTTGACATCGTTTTGGAAAAGAAGAACGACATTGTGTTGGAACACAAGGTGCGTTATAACTTTGCTTTGCAATTTCACTTTGAAACTACATACATAGGTAGCGGTGGCGAAATTAACCTACAGTTTGCACTGCAAGCTTCCACCACTAACTTTAGTAGTTTAGAGGAATTGCAAGCTAGCTTTAGTAAGGTAGGTAATAATTTAACTGCTCAATTGTTCTGAAAACCAGTTGTAACCAAGCTCACTTCTGGAGAAAATGACTTAACTCACATTGCTCAAACCGCCGTTGGGGAAAGTTTATTTGACAGCAGGGTTGACTTAACATCCTCTATTATTAACAACGAAGCAGCCATTAAAACTACTCAGCAACAATTTGAAACAGAGGCTTTAGCACTTTTTAAAGCAGAACGTGAAAAAGCTTTAGCGGAATATAAAGCAGAGCAAGAACGAATTGCTAAAGAACTTGAAGAACAAAGAAAAGAATTAGAACGTCTCAAAAAAGAACAACAAAATAAACAAGAGTTAGTAGAGTCCTTATACAATGTTGCTAACTTTGTTAGTTATTGAGAAAAGCGTGGCAAGGACGTTACTGACAAAAAACAATTAATTCAAGCACTTAAATCAGCTTTTGCTACCAACTGAAACGAAGTGTTTCAATTATTAACTGCTGGAATGCGTGAAGGTATTAAGGAGTATTACAAACATAATAAACCTGACCAAAGCGCTAATGCTAAAAAAGCCTTTGGCCAAAATGGTCTTGCATTTCCGCGAACTGGCTTTGATGGTATCTACATGTCTGACTGGCTTCGTGGTGAATTGAGGAATAAGGGTAACATCAATCTCCATCTCAAACAAAACGAAACCACCGTTAAAAAGATTAGGGATGACATTAGTATCGAATGAAATGAAAGCAAGGGCGGTATTGAGTTTCACCAAACTTACCCTTACTGATTTGAATTTGAAGTTAACTTTAAGTACATTGGTGGTTACTCCTTAAACTGATGGGATGCCATTTGAGCTAAAGTAGCTGGGATCCCAGGTTCTTGAAAGGGTGAAATGAACTTAAAGCTAGTTATTGATGGTGAAATTCATAAGTGAATGGTAACCAAGCCAGACTATCCTGGAACCTTCTTTCCATTTGATGACCAGTTTGATAAGCTTTGATTCACCTTGCACGTTAGTCAAGAAATTAGTGTAAGGGATGAATCCTTCATGAATTTGCTCAAGAAACAAGGATTAGATAAATTAGATCTTCGAACAGGATCAACCAAACCACCAGTAGTCGATCTAGCTAGCTACCTCCACTACTTGATTTTGGCAGACAAGAGTTAATAACTAAATTAACTATCAAAAAGTTTAAGGGCATCTACCTAAATAGGTGTCCTTTTACTTTACCTTTCAAACTAAACAAAAAATACTCTGTTGGAGTAGATTTTTTCCTCTCTAACCCTTTTAAGTATTGGTTGTAAGTGGTAATAAACGCTTCGTGATGAAAATCAAAAGTTCATTAGTTTAGGTTAATAAATTTAAGTAAAAATTAAATATGCTTAATTGATTTAAAAAAATTTTTTTCTCTGATTGAGGTGGAGCAGAAGATAAGCAGAATAAAAATAAGAAAAATGAACAAGCTGTTTTTGTAGATAAAGTAATGAGTAGAACAGATTTAACTGATGAAGAAAAAACAAATTCTAGTTGATTACTATCAGATGTTAGATGACTATTTGGACGATGATTGAGACTAACTAAAACCACCCCGTGGGGTAGTTTTTAATTTTAAGAACTAACCTTGTCTTAGTCTAAGCTAGCTCATTTTAGTGTTCTTTTAACAGCTTCGTTTCAACGCTTTGATTTCTTTTCCCGCACAGTTTGGTTCATTTGACTTTTGAACTGCTTAGCGATTCCAGTTAGTTTTTCTAGTTGTTTAGTGTCCTTTCAATAATTAACTGCTAAACCAGCTAGGAAGGCCGCACCCATGGCAGTGGTTTCCACATTCTTAGGAATAATCACCTCTAAGTTAGCAATATCAGCTTGAAATTGCATTAAATAGTTGGAATTGCAAGCACCGCCATCAGCCTTAATTTTTTTAATAGAACTGTTTAAATCACTAGCCATTGCCTTAATCAGGTCATTAGCTTGAAAGGCAATTGCTTCCAAGGTAGCTTTTACTAAATGTTCCCTTTTGGTATTAGCTTCTATCCCAAAGATGGCGCCTCTTGCTGAAGCATCCCAGTAAGGTGCGCCTAAACCAGTGAAGGCTGGGACAAACACCACTTCCTGTTCTTCCTTTTGTGCTAGCTTAGCATAAAAGTCACTTTCAGCTGCCGAGTACATTACCTTAAGACTATCCCTTAATCACTTTAAGGCTGCGCCAGCCACAAAAACACTACCTTCTAAGGCATACACCGGTTTTTGGTTTTCTAACTGCCAAGCTACAGTTGTTAATAAGTTATGTTGGGAGTATTTCAGTTCGTTACCGATGTTCATCAACATAAAACAACCTGTACCATAGGTGTTTTTAACCATCGCTGGTTCCACGCAGAGCTGACCAAACAAGGCTGCTTGTTGGTCACCGGCAACCCCACGAATAGGTACCATACCAGTAGCACTAGTAGATCAATGACTCGGTACTATGTCACCAAAGTGGGCATTGGATGGCATCACTTTGGGTAGGATTGATAAAGGTACTTTAAAGATGTCGCCTAACTCTTGTGATCATGTCATGGTGGTAATGTCAAACAAGAGCGTACGCGATGCATTACTTACATCAGTTACATGCATCTTACCACCCGTTAGTTTTCAAATTAACCAGGTATCAATTGTACCGGCTAACAACTTGTCTTCCTTAAGCATCTTTTGCGCTAAAGGAACATTTTCCAAGATTCAAGCAATCTTAGTGGCACTAAAATAAGGGTTAATTGGTAAACCAGTGTGTTTCTTGACAAACTCCTTTATCTTGGTGTCTTGGTTTAACTTATCACACAAACTAGCAGTGCGTTGATCTTGCCATACTATTGCATTGTAAACGGGCAAACCATTTTCTTTGTTTCATAACACAATGGTTTCCCGTTGGTTGGTAATCCCTACAGCAGCAATGTTGTTTGATTTAATTTGTGCCTTATTTTTAGCACTTTGCATCGTAGCTAATTGGACTGATCAAATTTCTAAGGGATCTTGTTCGACCCAACCACTCTTAGGAAAGAACTGGTTAAATTCGTTTTGGGCAATCGCTACTTGGTTTAAATCCTTGTCAAACACAATCGTGCGGCAAGAACTGGTTCCTTCATCTAGAGCAAGAATGTATTGTTGTTTTAGATCCATGGCAGATTGTACTTTCCGTTTCAGTTCGGCTTTTTAGTTAATTTCCAATTTAAAAGTCTGACAGCTTCCCGGGCAATGGCTGGTGCTGCAGTTAGCCCTGGGGATTTCATACCCCCTAAAATGACAAAGTCAGGGTCATTGTGAGCTATTCTAATAATAAAGTCATTAGTGGCTTTATCAATTGGACGCGAACCAGCAAAGCTTATTAAGGCATTATTAATGTTTAGACTTGGCACCATATGTTTGCCAATCTTAGTCAACATACATGGAGCATCTTTGGTAATGTAACGAGTAGCTTCCTTGTCTACCCCATCTAAAGCGGTGGGTCCGACTAAGAAGTTGCCATCTAACATGGGTGACACAATTACCCCTTTACCATGGATTGTTGGTACCATGAAAACCATTGAGTTTAAGTGGAGTTCGCCCTGATTAGTTACCACCACATACTGACCTCGACGGGTTGTTTGTTCAAAATCATCAGCTTTGGCTAAATGAGCTAAATAATCAGCATAATGACCTGCAGCATCAATAATCTTCTTAACTTTAAAACTAGGAGTAGTTTCGTTGTTAGTTCAAACTAAATAAGTCCCATCAACTTGCTTACTAATATTAGTAACCTCAGTATTAGTGCAAATTTGTACTTTGTTTTGCTGTGCTGCTAAAGCTAAACATTTGCTCGCTAACACTGGATCAATTGCTCAGCTACCCTCGACTTTTAATCCAGCCACAATTTCCGGATTAACGTAAGGCTCTAACTTTAAGGTTTCTTCTTTAGAGAGCATTTGAATATCTTCCAGATTAATTTGATTGGCAATGCCTCTTTGCTTTAGTACTTCTAATTGTTCGCGCTCCATTTCATTGAAAGCCACAATTAAAGTACGGATCTTTTGCCTTGGAAAACCTAAGCGTTTAAAGTAGGTATTTAATCAGAGCTTTTTACCCAAAATGTTGTATTTAGCAGTTAACTTGTGTGGATTGGGATCAATGCCAGTGTGGATAACACCACTGTTGGCATGGCTAGTCTCTTGTGCCAAATAATGGTGCTTTTCTACCAGTGTCACTTTGAGCTTGTACTGGCTCAATTCATAAGCAGTGGCACAGCCTATTACACCACCGCCAACTATTAAAACATCTCTTGTTTCCATAAAGGCTTAACTAATTAATAGTTACAGAAATGCCTTGACACGCCGATGCTTTTATCGTTTAAAGTTTTGCAGAGCTGAAAAAGTGGTTTTTTAAAAAACATTTAACTCTTATTTAACTACTTTGAAAATCAACGATCTAATAAGTTACTATTACGCATATTTTTTAGAAAGGGTGTAATTTTATGTTTGGAAAAGGGTTGGTCAAAAAAGGCCTTTTATTTTTTAGTGGCGTGAGTACTATGGCCGTCTTTTTAGTGTCTTGTGGTGCTACTAGAATTTGGGAATCTTCGATTCAACTGTTAGTTTCCAATGATGAAGCTACATTAGCTGACAAATCCTTTTCTGAGATGTCCTATGAAGGGATTAGAAGGTACTTTAGATCTCAAAAACATATTGAATTACCATCACCAAATAGTTCCCTGTTACAAGACGGCAACGGCCTTTGAAAACGTCCCGGACGTACTTTAAGTGATCGGATTGCTACCTTTAAAAACATTAAGAACGATGGTAGTGATGTGATTGTAGCTACTGGATTTAACCAACAAGAAGCACTACAAGCAATTAGCTCTGATGACCGTCGTTATTTAGCTGATAAAAACGACCTTGCTAAAGTTGGTTTCATTTTTGTCGATGGCCAAATTGAAAAGGAATATAACGTGATTAATAAAACGCCACAATTTAGATCAACCCCACTAAACATTTCATCCGTTGCCTTCCGCAGTGATGATGGTTCCTTCTTAACTGGAGTAGCAACTGCAGTTTATTTGAATTTAAATCAAGATTACTTTTTAAAGAAGAATGGAGCAACCAATAATAGTAGCCAAGATTTAACCGTTTCTGGTTTTGTCGGTGTTCCTATTCCTTCAACCCTTTCTTTTTTAAACGGTTTCCGTTTGGGCATTGCTTATTTTAATGAGGTGATTTACACCCATTTAAGTGATGCTGAAACAACATCTGATAACAAGAGTAATTCTTCAAGTGCTAGCAACTCTGTTTTAGTGCAACTTAAACAGATGCAAGGAAACGACAAAAAGATTAAAAAGATTAAATGGATATCTCCTAAACAAGGAAGCGATAATAATTCGAACCTCAGTATCGATGATCACAAGTCTGGTTCTTTCTCCAGCACTGAACCACGAGCAACAACTATCATTAATAATCTTTTAGACAAGGGTGTGAGTGCCATTATTCCTGTTGCCGGACCGCAAGTAAACTTAGCAGTTAACGAGGTGGCACGTCGCAAAGCGCACACTGCTATTATTGGTGTTGATAGTGCGCAAGAATTATTAGATATTAACCAAGATGCACCTGATAAAGATCAGTTAATCAAAGGTAATAAAAAAATTATTCCTTTCTCTTCTATTAAAGCCTTGGATGTTGCCATTGAAAACATGTTGATTGCCATTCAAAAAGGCAGTGATAATAATGGATATAAAGGATTTGGTTATAACAACATTGGTACGGTTGGAACTAGTTCAGTAGGTATTAGTGAGGCTGGTTATGAATTCTTAATTGATCCAGTTTTTTGAAAGACTACTCAGTCACAAGGGAAATCTATGGCCACAAATATGACTAATTTAAAACGTTTATCATCTGATGATACAAACACTAAGAAAGCATTAAAAGAGGTAAGCACCCATAAAAATGGTAGTGATAAAGATGGGATTATTGGTAAATATTCCAATTTATTAACTAAAAAAAGCACAACAGTAACTGCTGTTGCTCAAAAATCTATGACAGACAATAATAGTGGAACTGAGCAAAAAAAGAATCTTTCTGAAGTAGATACTAAGAAGAAAGAGAAAGAAAGTAAAGGTAAAACTCAAAGTAATGGTCAGGACAGTGGCCAACAAAACGGAAAAGAAACAAACGATATTATTTCTAAGTATTCTAAGCTGTTAACTATGACAACTATGAACAATAAAGTAATGTCATCTAAAAAACAAAGTAGCGATGATAACAGTTTTAAAAAGACTTCAGAAAACGGAGACTGAGTAATTAAAGGAGACGAGTTAACTAAGAAAAAATCAACTGAGCTTCCTGCTTTTGCTAAAGGTGCAGATTACCCTACTTTCCCTACAGAAGCGGTAAGCGTTATTAACGGAAGTACCGCTTTAGACGGCAAAGGTTTTAAGTGGAGCTTTAAACAAATTTAAAATAATACTCGGCTGATATGAAGAAGATTCAAGTAGTCGTTAAAGACCCTGTGGGCATTCACGCCCGTCCTGCTAGTATTATTGCTGGTGAGGCTAATAAATTTAAATCGGAATTAAAGCTAGTATCACCAAGCGGCGTTGAAGGTAACATCAAGTCGATCATAAACTTAATGTCTTTAGGCATTAAGCAAAACGACCACATTACCATCAAGGCAGAAGGAACTGATGAAGAAGAGGCACTAAATGCTATTAAAGCAGTTTTAGAAAAACACCAAGTTATTTAAGTAATTTAATTTAACTTAGAATAAAAGCAACAAGCCAATCCCCAAGGAAAAGCTTGTTGCTTTTTTATTAAGAAATATGCTTTACTACGACGACGAGGATGAATATGACACTTCTAATTCTGGTGGTGTCGTAGATTTAGCATTTTTAGCACTAATAGTAGTTTTTCTATTTACCGCTTTTATTTCTTGGTTTGGGGCATGAACTTGCTACAAAACAAGGCGTTTTTGAAAGGTAATTAATAAACCCATTAATGTAATAGCAACAGCTTTCTTTAATTTGGGGAACCTTACTTTTTCTTTGTAGCATTAACTTTTCTACTAGGAAATATACAAGCATTTACTCATGCTAATTAAGTAGTAGCACAGAACTTCAGGGAATAGCAATTAATATTGCCTTAATTGTTGGCATCATTAATTTACCTTTATTTATATTCTTCCGAATAATGCTGAAAAGTACTCATAATTTCTTAGACAAAAACATTCCACTACGAAAGAAATGAATTGAAGAAAAGATTGCTAGTGATGAATTGGAAGGATGAGAATACACCCAAATTTATCCTTTCCTAAGAAAGCTTTCTAGAAGAAAGCGTTACCATTTTCTAAGAGCCTTAGAAAATGGTGATAAACCAACTTCTGTTTTAACAGATAGAGAAAATCAATTGTCTGAAAATCAAATAAGTCAAGCACATGCTTAGTAAATAGACACTTAGCAAATTTATGTTTGCAAGTCTTTTTAATTTTTTAGCATCAAATTCGTCAATAAACTCTCTAATTTTTACCTAAAAATTCCTTATTTATTTCCTTATATTTTTATTTAAGAATAATCTTTTTTATTTCTCAAAATTTCCTTGATTTTATCCAAAATTTCCAATTTTATTTCTGGGAATTTTTACTTGTTTTATCTAAAAAATTACACCTTTCTTTTTTAGTGTTTCTCAAATATTTCTCTAAGAATGAGATTTTTATTCTTTGGTATTTCCACGTTATTTATCGAAATAATCTCTTTTTATTTCTAGAAAATTACTTTGATTTTTGTCTAAGGATTCTCAACTTAGTCCCTAAATTTTCTCCGCTTTTTAGCGAAGAATATTTCAATTTATTCTTTGATTTTTATCTAAAAACTATCCCCTTAGTTACTTTAAATTTCTGTTAAATTTGTTTAGGGACTCTCATTTGTTTCTAAAAAACTTTTCCTTTTTCTTTCCAAAAGATTACAGTTTTTATTCTTAGAAACTTATCCGCCGTTTATCACAATATCTTTTTATCAAAACTTCTCCTTTTTATTCTTTAGGTTCCTCCTTTAATTTATCGAAATTTTCCATTTTTTCTTTTCTGGAATGTTTTTCTTTTTATAGCTAACTTAGTCTTTGAAATTTCTCTTTTTTATGTTCGAAGATTACGAATTGATAGACAAGAAATTGAAGATTTTTCAATTTTTCAAGCCTCCTTTTTCCAAAAAAACAGAATTGGTGAAGTTACTTTTTTAATTTTGTTCTAAAAGTAGCTTCGTTACCTTTCTAATTTAATGAAGATTAAATATTTCTTTATTCCCTTGTTTTCTTCAGTTATATTATTTAGTGCCTGTTCTTCTATTCAGTCTGATCTTAGGAATCTTATTAAAGAAACAACTGGCAAAGATTTTGACGTATCTAAATTAATTAAAACCTCTGAAGGAAGAAAAAACTTAATAAATTCTCTCAAAAAGTCTTACGAATCTAAGCCCGATGAAACAGCTAGCTTATTACTAATTGCTTGAAAGCAAAGTGCAGAAATGGGCGAAATAGGTTTTGATGATATTAAAGAAGGAGGAATTTATACCAGAGACAATGATCCTTTCAAATTGGAACAAAAGGTTGAGTACTTCAATATGGAATACAAAATTTTAGTGATGTTTTAATTAAAGCTAAATTGATCTATATCTTTAAATGGCATGGCGATCATTCTTCTTCAGAAGGCTTAACTGCTAAAAATGGCGGCAAGCATCATTTCGATCTTTTTCTTAATCTTAAATCCTCTTCAAAAAAACCATTTAATTCAAAAAGTTTTGATACAAAATCGGGAGAAGTTTTTCAAACGGAATGAATTAGTTTCCATACTTTAGCTAGTTGATCATTAAAAGGTAAAGATGAAAAAAGTCAAAAAAATCTTGACAAAATTATCATTAGCGAGTTTGTTACTAAAAAAGACCATGAACATGATTTTAATCTCTTTATATATTTAAACTATTCAATTAAGTAAGCTCTCTAGATTATTTAGTTTTGATTTGCTAAAAATTCAAGTACTTTACTTTCAGTTCAGTCTTTAAAGTTAGCTATTTTTTAGATCAAAGATTAAATTTATTGGATTTCTATCAAAAAATTCCTCTTTTTACTCTTTGAATTTTCTCTGTTTTTTATCCAAGAATTACACCCTGATTCCATTAAAAGTTTTTGATTTTTACCAAAAAATTTCATTTTTTATTTCTTAAATTTACCTTTCTTTTTACCTAGGCATTACCCCCTTATTAGCAGAAGTTTCTACTTGCTTTAACCAAAAGATTTCCTTTTTCTTCTTTAAAAATTCGCTTTACTTCGCCTAATATTTACCCTTTTTTATTGAAAAAATTCCCTGATTTTAGCCAAAAGATAGCTTTTTCTTTGTCTAAACTTTTAGCTCTTTTTTTACCCAAAAATTTCCTTTTTGCTTTTTTCTCCAAAATTTCTCTTTTTTTGAATTAGAAGAATGCACTTTTTTATTCAGAAGTTTTCTGCCTTTTGTCTTTTAAATAATTTCTTAATTTTTATCGCAAAACTCTCTGTTTTGTTTCCTGATAAATTCTTAGTTTTTATCTAAAAGTTTCCACTTTCTCTTTTAAGAATTTCTCTACTTTTTATCAAGAAAGTTTCTTTCTCTTGTTTTTCTCTAAAAATTCCACTTCTTATTCCACTTATTTTCAGTTTTAATTTCTCCTTTTATTTTTGAAATAATTTCCTTCTTTTATCAAGTTCTTAACCTTGTTCTTATTTGTTAATTCCCCTTCTTTTAGCCTACAACTTCTCTTTTTTCTTGTTAAAAGATTCCCTTTCTTTTTATCAAGAAAATCTCTTTTTTTATGTCTTAAAAGTGGTCTTTATTTTGGTTCAAAATTTAGTCCAAAAACAGTCAAATTTTTAGCACAAATTTAACTGCAAAAACTGTTCAAAAAATCTCACAAAATTGCAATCATTTTTTTGCAAGAAAATTTATCCCAGTTTTAGGGGGCAAGCGGGGTCAAAAATTGCTACAATAAATACCGTTTTTTCTTTAAAAATTAAGTAGTTAAATTGAACGAACGTTTTTTAATTGAAATCGAAGGTTTAAACAAAACTTTCGATGATGGTTTTGTATCAGTACGTGATATTAACCTCAAAATTAAGAAGGGCGAATTTATTACGATTCTCGGTCCTTCTGGTTGTGGCAAAACAACTACTCTTAGGTTGTTAGCAGGTTTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTCTTGACATTAAAGACTTGCCCATTCATAAGCGTCCCTTTGCTACTGTCTTCCAAGACTATGCCTTGTTTTCACACTTAACTGTGTACAAGAACATTGCTTATGGCTTGAAGGCAATGTACACAAAGCTGGATCCGATTGATAAGCTAGTGGAACAATACCACCAAAGCTTACTGGACAAGCAACACCGCTTGCACAAGAGGATTGAACGCTTAGAAAAAAGTAATGCTAATCCACAACTGTTAGAACAACTTAAACAAACGGTTGTCGTTCAACAAAAACAGTTTAAACAACAAACAGAGACTTTTAAACAAAAGGAAAATGCTCGACGTGATGCCATTCAACAACGCTTGGTGCAACTTACCAAGGAATGGGAAAGCTTATCAAAGCAGAAGTTGCAACAATTAGAAGCGGAGAAAAAGTTGTTAGATAAAAAGTTTGAACAAACTGAGCGCAAATACCAAAAGGATGCTTGGATGGCAACCCACAGTGAAATGCGTTTAAAGCAGTTCAAACAAGAGGTGTTAGCTTTAAAACAACTAATTAAAACTAAGTTCAAACAAAATGAACCAGTTGACAAGTTGCAGTTAAAGCTGCAAACCTTAAAGCAAAAGTATGCTGCTAAAAGACAAATTGATAAGGAATACGATAAGTTGGTCTTAGCCTACAACAAGAAGGACTATTGAACTTCATACTGGGAAACCTATTCTTTACAACAACAAGAAGCCTTTGAAAAGCGTTACTTATCGCGCAAACTCACTAAACAAGAACAACACCAAAAGGTGTGTGCTGTAATTGAGTTAGTTGGTTTAAAGGGTAACGAGGACAAACTCCCAGAAGAATTGTCTGGGGGAATGAAGCAACGGGTAGCCTTAGCGCGTTCCTTGGTAATTGAACCAGACATTCTCTTATTGGATGAACCCTTGTCTGCTTTAGATGCTAAGGTACGTAAAAACTTGCAAAAGGAATTGCAAAAGATTCACCAACAAAGTGGTTTAACCTTTATCTTAGTTACTCACGACCAGGAAGAAGCCTTGGTCTTGTCCAACCGCATTGTGGTAATGAATGAGGGTAACATTCTCCAAGTTGGTAGTCCGGCTGATATTTACGATTCACCCAAAACCGAGTGGATTGCTAACTTCATTGGTCAAGCCAATATCTTTAAGGGTACTTACCTAGGTGACCTTAAGATTAAACTGCACAGCGGTGAAGTGATTAAAACTGATGTGGACAATAACTATGTGGTTGGTAAGGAATACAAAATCCTAATTCGTCCCGAAGACTTTGACATTGTGCCCAAAAACAAAGGCTTCTTTAATGTTCGGGTAATTGATAAAACCTACAAAGGATTACTCTGAAAGATTACTACTAAGTTAGTTGATGACACCATAGTAGATCTAGAGAGTGTCAACGACATTGAGGTTGACAAAACCTTTGGGGTCACCTTTGATCCGATTGATGTTCACCTAATGGAGGTATAGGATGAAACTATCGAAAAAATACCTCTTAGCTGTACCATTCTTTGTTTTAATGGTCATCTTCTTTGTAGTACCAATGGCATGGATTATTGTTAGTGGACTCCAAAATGAAAACGGTGCAAGTATAACGGAAAAGTACCAACCCTTAGTTGGTGGTTATTCTTTCTTTCAGAGTTTTTGAACTAGTCTGTGAACGGCGACTGTGACAGTACTAGTAGCCTTACTGGTTGCCTTTCCCTTTTGTTACTTTTTATCACAAAGTAAAAACAAGGTATTCCGCAGTTTTGTGATTGCCTTAGCTACTGCTCCCATATGGAGTAGTTTTCTCATTAAGTTAATTGGGTTAAAAACACTGTTGGACTTAGTTTTGGGGTTAGCGTTGAACCGGGTGGGTGATAATAACCTCACCTTTGGTAGTGGTTATACCTTGATTGGGATGATCTACCTGTTCACCCCCTTCATGTTTTTACCACTTTACAACAACTTTTGTATCTTACCGAAAAACTTAATCTTAGCTAGTCAAGATTTGGGTTACAACTGGATTACGAGTTTTATTAAGGTAGTTATTCCCTTTTCTAAAACTGCCATTCTGTCAGGGATTGCGTTAACTTTCTTTCCGTCTTTAACCTCGGTAGCGATTGCCCAGTTCTTGGACAACTCGAATCAAAATAACACCTTAGGTAACTATGTCTTTACTTTAGGTAACAATGGTTATGACAGTGCGATTGAGCGCGGCCGTGCTTCTGGTGCCATTATTATTGCGGCTTTAATTACCTTTGCCTTTTACTTTGTGGTGATCTTTGCTCCAAGAATAGTGCGCTTAATCCAGACTAAGTGTTTGAAATACAGGAGGGTTAATGTTTAAGATGAAAAGCTGTAAGTTATGACTACGGGGAAGTTTCTTTGTCATTGTCCTTGTTCTCATCTACCTCCCTTTAATAATAGTTGTCTTAGTTAGTTTTAACGGTTCTTCGACTAGGGGTAACATTGTCTTAGACTTTGGTAATGTTTTAAACCCTAACCCGGATGCCAAGTCAGCATATCTAAGATTAGGGGAAGCGGACTTTGCCATCCCTTTACTCAACTCTGTCATTATTGGCTTAATTACGGTCATTGTCTCGATCCCGATTGCCATCATGACTGCCTTTGCTTTATTGCGATCCAGGCAATGGCTCAATAAAACAGTCTTTGGGATTGCTAACTTTTCTCTAGCAACTCCCGATATTATTACCGGGATATCGCTAGTGTTGTTATTTGCCAACACCTGGTTGAGCTTTAACCAACAGTTAGGTTTCTTTACTATTATTAGTTCCCACATCTCCTTTTCGGTGCCTTATGCCTTGGTGTTAATTTACCCAAAGATGCAAAAGTTAAACCGTAACTTAATCCTAGCGAGTCAGGATTTGGGTTACTCACCCATTGCTACCTTCTTTCACATTACCTTACCTTACTTACTACCGAGTATCTTGTCAGCCATTCTAGTAGTGTTTGCCACGAGCTTTGATGACTATGTGATTACTTCCTTAGTTCAAGGTTCAGTTAAAACAGTCGCCAGTGAGCTCTATTCCTTTAGAAAGGGGATTAAGGCCTGAGCTATTGCCTTTGGTGCCATTTTAATCTTGGTCAGCATTCTAGCTGTCTTGCTAGTAACACTGCACAAGTACCTACGCTTTAAACATAAAGAAATGCTCCGTGTTAAACAATGAAAAAACAGTTAAAGTATTGGTTTAGTCTAATGGGACTAACCATGTCAGTTGGGTTAACTGCTTGTAGTTCCAACCAGTTTGTGTTTGCTAACTTTGAGTCGTATGTGTCGCCCTTACTTTTAGAAAGGGCAGAAGCCAAGCAACCAATGACCTTTCTTACTTATCCTACTAACGAAAAGTTAATTAACGGATTTGCTAACAATACCTATACGGTAGCAGTGGCCTCGAGTTATGCTGTGAGTGAACTGCAGCAACAAGGCCACTTACTACCAATTGATTGAGCTAAATTCAATTTAAAGAAAACCCAAAATGGTAGTAACCAAGCCACCATTCAAAACAAAGAGGATGCCAAAGAATTATTTACCAAAGAAATAGGCGACATTAGTGGTGAACTTTTAAACTGGGGTGTGCCCTACTTTTTACAAGACCTCGTGTTTGTTTACCGGGGCGAAAAGATTCAAGAACTTGAAGGTCAAGATGTTACTTGATCAACGATTATTAAAGCTATTGTTAATCACAAAGACCGCTTTAACAACAACCGTTTAGCCTTAATTGATGATGCTAGAACAATCTTTTCCTTAGCGAACGTCGTGCACCATGAGGTTAAGAATACCACGGTAGATGTTAATCCTACAGGTAGCACCTTAAACTACTTTGGCAACGTTTACGAAAGTTTTGCAAATTTAGGTCTAAAACGTGATAACCTCAACACGCTCTTTGTCAATTCGGACTCCAACATTATTATTAATGAATTAGCTAATGGCAGAAGACAAGGTGGCATTGTTTACAATGGTGATGCCGTATATGCCGCTTTGGGCGGTGATCTCCGCGATGAAATTAATGAGAATAACTTGCCCAACGGCGATAACTTCCACATTGTGCAACCGAAACACTCGCCTGTGGCCCTAGACTTTCTCATTATTAACCAGCAACAAACTCACTTTAGAGATGCTGCACACCAGTTAATTTACCAGTTGGCCTTAGAAGGCGCTGATCAAACAGCAGAAGAATTATTAAAAACTGATGAAGAGAAAGGTACCAGTGATGAGGACTATTACACTTATGGTGCAATGCAGAATTTTAGTTACGTTAACTATGTTTCGCCTTTAAAGAATATTTCTGATGAAACCACTGGGATTGTGTTTAAGGAAAACAAACAAGCTGATACTAAGCGAGTTGTCAAACAACAAAGTCAATCAGAACAACAATCTGAATCAGCAGAAAAGGAAGAAACGGAACAAGATGATTTCTACACTGCTACTCTCAAAAGTTTGTTGAAAGCGGACAGTTTAGATGACAAAGCTAAGAAGTTAGTAGACACAATTAAAAAGACTTACAAGATCAAAAAAGCCGACAATATTAATTGGGCTAACTTAATCGAGAAACCAATTACCCCATTACAAAGGAGTAATTTAACATTATCGTGGCTTGACTTTAAGCAACGCTTTTGATAATGGAACAACCTTTGTGTATCTTGGGAATTGAAACCACTTGTGATGACACTAGCATAGGGGTAATCACTGAGAGTAAGGTACAAGCACACATTGTTTTATCATCCGCCAAATTACACGCCCAAACTGGTGGCGTAGTGCCCGAAGTTGCAGCTAGGAGTCACGAACAAAACTTGCTTAAAGCACTGCAACAATCAGGGGTTGTTTTAGAACAAATTACTCACATTGCTTATGCCGCTAATCCGGGATTACCCGGTTGTCTGCATGTGGGAGCTACCTTTGCGCGCAGTTTAAGCTTCTTATTGGACAAACCCTTATTGCCCATCAACCACCTTTATGCCCACATCTTCTCGGCCTTAATTGACCAAGATATTAATCAGTTAAAACTCCCCGCTTTAGGCTTAGTGGTGTCCGGGGGGCACACTGCCATTTACCTAATTAAGTCCTTGTTTGACTTGGAGTTAATTGCTGAGACTAGCGATGATGCGATTGGCGAAGTTTATGATAAGGTGGGCCGCGCTATGGGTTTTCCTTATCCAGCTGGACCCCAGTTAGATAGTTTGTTTCAACCGGAGTTAGTTAAGTCACATTATTTCTTTCGTCCTTCCACGAAGTGAACGAAATTTTCCTACTCGGGGTTGAAGTCACAGTGTTTTACCAAGATTAAACAGTTAAGGGAAAGGAAAGGCTTTAATCCCCAAACCCACGATTGGAATGAGTTTGCTTCTAACTTCCAGGCCACCATTATTGATCACTACATCAATCATGTCAAGGATGCCATTCAGCAACACCAACCGCAAATGTTGCTGTTAGGTGGTGGGGTAAGTGCTAATAAATACTTAAGAGAACAGGTAACACAGTTACAGCTGCCTTACTTAATTGCGCCGCTCAAGTACACGAGCGATAACGGGGCGATGATTGGTTTTTATGCTAATCTTTTAATTAATGGCAAAAACAATTAAACATCCCCGATGGGGTAGATATGTAGCAGAAGCAGTTGGTCGTGGTCACCCCGATAAGATCTGTGACCAGATTGCGGACAGTATCTTAGATGAATGCATTAAGCAAAGTCCGACCAGTCACGTTGCTTGTGAGGTCTTTGCTTCCAAAAATCTGATTATGGTGGGCGGGGAAATCCTTACCACTGGTTATGTTGATGTGGTCCAAACCGGTTGAAAGGTATTAAATCGGTTGGGTTACACCGAAAATGACTTTAGCTTTTTGAGTTGTATTAACTCCCAGTCCAGTGAAATTAACCAAGCTGTGCAAAGTAATGACGAAATAGGCGCGGGCGACCAGGGCATTACCGTTGGTTATGCTTGTAGTGAAACCGAGCAATTAATGCCCTTAGGGAGTATCGTAGCGCAAGCGCTGGTGCAACGCGCTGCTCGTATCATTGACCAATACCCCTTCATTAAACACGACATGAAGAGTCAGGTAGTGCTCAACTATACTGGTAACAAAGTTCAATGTGAATCAGTTTTAATGTCGGTACAACATACTCAGGACGTAAGTTTAGACCAACTGCGCCAAACAATTATCAACCAAGTGATCCTCCCCGTATTAACGGAGTACGGTTTAAATGATCCTAAAATCAAACACCTAGTCAATCCCGGGGGTTCGTTTGTCGTTGGGGGCCCGATGGCCGATACGGGGTTAACCGGACGCAAGATTATTGTGGACACCTATGGTCCGTACGCTAATCATGGTGGGGGGAGTTTTAGTGGTAAAGACCCTACCAAGGTCGACCGCACTGGAGCTTACTTTGCGCGCTTTATTGCTAAACACATTGTTAGTTTGGGTTGAGCGGAAGAGTGTGAAGTGAGTATTAGCTGGGTCTTTTCCCAACCATTACCACAATCAATTCAGGTCAAATGCTTTAACATTAATAAGGAATTTAGTGAACAATTAATAAACCAAGTTATAAGTCAATATTTCAATTGATCTGTTGCTAAAATTATTGCAAAGTTAAAACTCCTTGATCAAGTTGAGTACTTTAGGTATGCTGTTTACGGTCACTTTGGTCACCAAACAGCCCCTTGAGAACAACTAAGTGAACGTGACTCGCTGCAATGTTTAATCAAAAATTTCCAATAAAAGCTCCTAAAGCACCATTTTTTAAAAAGAAAACACCACCAGTACCACCAAAGAATCCTGTGGTAACCAGCACTACCCCAGAACTCAAGAAAACCAAAATTAAAAAAGTAAAGGAAGCAGGGGATACCAATCTTTGAGATAAGATTAAAGCAAAGGTAAAAAAACACCCTTACCTGTTTGCTGTTTTAGTGTCCTTTATTCTTTTAATTGCACTTTTAGGCTGATTATTTGCAATTCCGATTTCGCAAATTCCTTAAGCTGCAAAAAGATAAAAAAGCAATGTTCGCTTAAAAACCTAAGCCACCAAGTCCACCAAAGCCACTAAAGGGATTTTTACCACCTTTCAGCATCCGGCCCATTTCTGCTGTTTTCGTTTGCAGTTTACTCCACTCCTTCATTAGCTTATTGAGTTCATCCATTTTACGTCCCGAACCCTTAATGATGCGTTGTTTTCGCGATGGGTCACGATTAATTAATTTGGGGTGCCGGCGTTCTTCACGCGTCATTGAGTTAATTAATACCTTTCACAGTTTAACCTTGGCTTCAATGCTCTCTACATGATCATCCGATACCGTTAAGTTACCCGGGAGCATCTTGACAATACTACTAACAGAACCCATTTGGTTCATCTGTTCCATGTAAAGCAACAGATCTTCCAGGTCCATCTTGCCCAAGAACATCTTGCTAACTGTTTTGGTTAAGCTCTGTTTGTCAAATACTTCTTCCACTTTTTCCACTAAGGTCATCACATCCCCTAAACCCAAGATCCGACTGGCAATCCGTTCTGGGTGAAATTGTTCTAGACCTGTGAGTTTTTCTGATGTCCCCATTAGTTTAATCGGAACTTCCAACAAACTCGTTAAGGACAAAGCCGCTCCAGCGCGGGCATCACTGTCAAGCTTGGTAATAATAAAACCGGTGAGTTTTAAACGGTTATGAAACTCCTTGGCAACATTAATAATTTCCTGGCCACTTAAACCATCGACCACCATTAGGATTTCATCGGGGTGGAGTTCGTTTTTAATTGCTACCAACTCATCCATTAATTCCTGATTAGTTTGTAACCGTCCCGCCGTGTCACAGATTATTGCTTGACAACCGCTTTCCTTAAAGGTCTTAACGGCCTCCTTGGTGGTTTGGTCGGGGGCTTGGGTACCCTTGGCAAAGAAGACAGACTCGGTTTGTTCGGCTAGGGTCGCTAACTGGTCAATCGCAGCGGGGCGGTAGATGTCTAGTGCCACTAACATCGCTTTTTGTTGAAACTGTTTTTTCAGTCACACCGCTAACTTCCCACAGGTAGTGGTTTTACCTGAACCCTGTAAACCGACTACCATTACCTTAAGTGGACGTTTACTAGTGATTTCTTGGTTGGGTTGGCTCAAAATGTTGATTAACTCTTGTTTAATTACTTTGAGCAGTCACTTCTGTAAGTCCTGACCGGGTTCTACGGTTTGACCAACGGTTTGTTCGCGAATCGCCTTAATGAAGTTTTTGACCACCAACAGGTTCACGTCAGCGTCTAAAAGAGCAATCCGAATTTCCTTTAAAACGGCTTGGACATCGGTTTCACTGATCGTTTGCGCGTTGATCTTTTTTTGCATCGAGCGCATAACGATGCTCGAGATCATTGACTTAAACATGGTGCTTTAATTATTTATTTTTTTATTATTTCTACGTATAGATAAAGCTAGTTTCATGACACCGCACATAAACGCAAAAAAAGATGACATTGCTAAGGTTGTTTTAATGCCCGGTGATCCCTTACGTGCTAAGTGGATTGCGGAACAATTTATGGAAAAGCCCCGGCTCGTCAACGAAGTGCGGGGCATGTTAGCTTTTACCGGTCAGTATAAGGGTAAGACCATTACCATTATGGGCCATGGCATGGGGATTCCCTCAATTGGGATTTATTCTTATGAACTGATGAAGTTCTATGAGGTTAATACCATTATCCGAATTGGTAGTTGCGGGGCGTTGCAAGATTCCTTAAACCTCCAGGACCTCATTATTGCCGCTAAGGCTTGGAGTGAGTCGATTTACGCTAACGACATGGGTGTGGAAGTGCCAGCAGACAAGATCTTAATGGCCTCCCCGCAACTAGTAGAGTTAGCTAAAAAGACCGCTAACCAACTTCAACTGGCCTTCCATGAGGGCTTGGTGTTCTGTGAGGATGCCTTCCACCAAACCAGGAAAGACGTGTTAAAGCTAGCACAAGAAAAACACGCTTTAGCAGTGGAAATGGAAGCGCATGCTCTGTACGCTAATGCCATGCTGCTCAACAAGCAAGCGTTAACGATGCTCACGGTGTCCGATTCTTTGGTAACCCATGCTGCTCTACCAGCGGAACAACGCCAAGCCACCTTTAAGAACATGGCTATCTTATCCTTGGAAATGGCTAGCCAACTCGTTTAAACAATGAAATTAGAATACAACCGCATTATTGACAGTACACTACTTAAAGCTGATACCTTACCCCACGAAATTGGTGCTTTATGTGCTGATGCTCATAAGTACCAGTTTTACGCAGTTTGTGTCAATCCTAGCTATGTGCGGTATGCCAAAAATATCCTTAAGGGTACGGGGGTGAAACTGTGCACTGTCGTTGGTTTTCCCTTAGGACAAACAACACAACGCCAGAAGGTGTATGAAACTAAGATTGCCATTAAGGAAGGTGCGGATGAAATTGACATGGTAATGAACATTGCCGAGTTCAAAAAGCGCTGTGCCTGTGTCATTAGTGAAATAAGGGCAGTCAAAAAGGTTTGTGGTAAGCGAACGCTCAAAGTCATTATTGAAACGGCTTTGTTAAACCAGGACGAAATTCGTGATGCCGTTAATGTCTGTATTGATGGTAACGCTGACTTTGTCAAAACTTCTACCGGGTTTTCCATGCGTGGTGCATCATTGGAAGATATCACAATTATGCGCGAAGCTAGTGGTAATCTCATTAAGATCAAAGCATCGGGTGGGGTTCAAACAGCGCAGCAATTCCTTGACTTCTTTAATGCTGGAGTTAGTCGGATTGGCACTTCCAATGCGGTCAAGATTATGGAAGAATTGCACAAACTAGAAAGCCATGAACATCGTTAACCTTATTAGTAAAAAGCAACGCGGCAAGGCGTTAACGGAAACAGAAATTAACTGGTTTGTCCATTCCGTTAATAACAAAAGCTTGGCCGATTATCAGGTCAGTGCCTTTTTAATGGCAGTGTGATTTCAGGGGATGAATTCCAAGGAGTTGTTTTGTCTCACCAAAGCGATGGTGAAATCAGGAGAGAGCCTCCACTTTAACCACCACAGCAAGTTAAGTGTTGACAAACACTCCACTGGTGGTATTGGTGACAAGGTTTCCATTGCTTTAATTCCAATCTTAACTGCCTTAGATTACAGTGTCGCAAAACTGTCGGGTCGTGGTTTGGGCTACACTGGTGGCACAATTGACAAATTGGAAGCAGTGGGAGTAAAAACAGATTTTACACCAACAGAGGCGCAGAACTTACTAGACCAAAATGACTGCTTTATTATTGGTCAAAGTGAGGGCATTGCTCCCGTAGACAAGGTGTTGTATGCACTACGCGATACTACGGCAACTGTGGACAGTCTGCCCTTAATTGCTTCCAGTGTCATGTCGAAGAAGTTGGCAATTAACAACGACTACATCTTCATTGACCTTAAGTACGGTAAGGGCGCTTTCTGTAAAACGAAGACGATGGCTAAAGAGCTAGCGCAGTACATGTACAGTATTGCCAAGCAGTTTAAGCGCAAACTCTACATTAAACTAAGTGACATGAACCAGGTTTTGGGTAAAACGATTGGTAATGCGTTAGAGGTCTTGGAAGTAGTGCACTTCTTAAAACGGAATTGAACTGAAGTGGGTGCTGACTTTATTCAGTTGATGGAGCAAATAGTCACTGAAATCCTTATAGAAACAAAACGTGCACCAAACAAACGGGCAGCTGTAGCACTTTACCACGCTACATTAGAGGGCGAAAAACCATGGCAGCGTTTTTTAAAATTTATTGAGCTGCAAGGTAGTAGTCGGGAACGTTTTTTGGATTTAAAAGAGCTGTTTAATCCCCAGTATAAAGCGCCAGTGTTAGCATCTCAAAGTGGTACTTTGAGCTATACCAGTCCGGTGGATTTAGCCATGGTCTCGATTAGCTTAGGTGCTGGTCGAATGGTTAAAACAGATCTGATTGACCCAATGGCGGGGATTAAGTTAGTGAAACAAGCTAACGAAGTGGTAAAAGCTGGGGACACAGTCTTAGAACTTTACAGCTCCAAACCAATTACGCCTGCCCACATAGAAGCTGCGCAGCACACCATTATTATTAAGCAGTAAATAAACCTCGATGAAAGTTGATTTAGATTGAGTCCACCACAAGTTACAGGAGGTGGTCAATCATGCCTACACGCCTTTTTCAAAGTTTAAGGTAGCTTGTATGTTGGTGGCCAATAACCAGGCATTTTATGGAGTCAATATTGAAAACGCTTCTTACCCAGTAACCCTGTGTGCGGAACGTAGTGCGATTGCTAACATGGTCACTAGTATCGGCAAGGCCACAATTGATTATGTCTTTGTGTACTTTGACACTAAAACTCCAACCAATTCACCTTGTGGCATGTGTCGGCAAAACATCTTTGAGTTTGCCACTGATAAGACCCAGTTATTCTGCATTGAGAAGGACAAGAGCTTTAAACAATTTACTATTCCCGAAATTTTAAAGGGAGGTTTTCGATCATATGAACAGTAACGCATACTTGGAAGCTCAAAGGTGGCTAAGCCATCCGCGGGTAAAACCCAACTTAAAGGAGGTCATTACGGCTATGAGTGCTGAGGAAATCGAGCACTTCTTTAGCTTAAAGAAACCGAGTTTTGGCACTGCTGGGGTACGGGGCAAAATGGCACCGGGGTACCATGGGATGAATGTGTTTTCCTATGCCTACTTAACCCAGGGTTATGTCAACTACATCCAGTCGTTAAACCCAACTAAAAAACCATTACGCTTTTTAGTAGCCAGAGACACTAGAAAACACGGGGCATTGTTTAATGGCATTGTGTGTGATGTGATTACCTCCATGGGTCATGTGGTCTATATGTTTGATAACAATGAACCAACACCAACACCATTAGTATCTTATGTGATTAAGAAGTACCACTTTGATGGTGGGGTGAATGTCACTGCTAGTCATAATCCTAAAACCGATAATGGCTTTAAGATTTACGATGGTCATGGCGCGCAGTTACTAGACTTCCAAACTGATCAATTAATCGCAATGTTACCCCCAGTGGTAACAATGTTAGATTTCGAACCACGTGGTAACAACGAGCTGTTACACTTTTTGGATAACGAAGTGGTCTACAAAAACTACTTTGATGATCTTAAGGAAAGCCTAGTAGTGGACAACGACAGCTTTAAAAACCTCCCGGTGGTCTTTACCGGGTTACATGGTACTTCGGTGAAGTTGTTACCAAGGTTTTTAACCTATTTGGGTTACAGCAACATTATTAGTGTCCAACCACAAAACGTGTTTGACGCTAACTTTGCTAATGCTGACCACCTCAACCCCGAAAGTAAGGATACCTGAGAGTTAGCGCGACAGTATGCATCCAACACTAAGGCCAAGTTAATGATGGCAATTGATCCGGATGCTGATCGCTTTGCGATTGCCGAATGAAATCCGCAAACCCAAGACTGGCACTACTTCTCCGGGAACGAGTCAGGAGTTATGGTGGCTTACTATAAGCTCAAACACAAGCAGTTTAAGCGTCAACCTTACATTGTAACGACGGTAGTCTCTACTGATCTAGTGGATAAGATTGCCAAGAAGTACGGGGCTTTTGTCAAGCGCACAAATGTCGGCTTTAAGTTTATTGGTCAAGCTGTTGATCACTTCTCCAAAGACAATGAACTAGTAGTGGCCTTTGAAGAAGCGATTGGTATGATGGCTAGTGATGGTTTGAACCGCGAAAAGGATTCCTTTCAAGCAGCGGCCATCATGCTTGAAATTGCGCGCTACTGTCATAATAAAGGAATTAGCTTACTAGAGTTCTACCGTGGTGAGATCTTCGGTGAATTTGGTGATTACTATAACTGAACGGTACCACACACCATTCACGGAGTTAACTGGAAGGAAAAAATGGAACAGGTATTACACCAGTTAACCACAGCTACCATTAAAGAGGTTGTGGGACACAAGATTACCAAGATTAAAAACTATGTCGACATTAACCTAGTCGAATATGTCTTGGAAAACGGTAACTGGATTAAGTTCCGCATTTCCGGTACAGAACCGAAGTTAAAGCTGTACTTTAACCTATCTAAAGGTTACTTAGCAGCTTTAAAACACGAAGCGAAAAAGATGCACGAGTTCCTAGTGCGATTGCTCAACCTCGATAAAGCTTAGGTTTTCTAAAGGCAAGTTAATTAACTGCGTTTTGGTGGTTAATTCGACTTCCACTACCGCTTTGTTAATTGTTCCTTGGTATAGAGCCTTAATTTGACCTTGAATGTGAATCGCACCAGTTAAGTGGACTTCCACTAAGTTACCAACACTAAATTGTTGCAGTTGATCGACCTTAGTGTCACCTTCGACAATTTCGTATTTACCCAAGTAAGGAATTAACACTGATGGGTCAACCTTGTTAGCTTCACTTTTAACAATCGCTACATCCTCGTTTCATTCGTCTTGTTCGGCCTTATTAGCTTCCATTAGAGCTTGCGCAAACTGGTTAGTATCAATGATTTCACCAGATTCGTTAACTTGGGCAATTGCTTCGGGTTTAACTTGTTTGTGCTTGAGAATGTACTGAAACTTTTCGTCAAACTTGTCTGCATCCATTTCCACAATGGCAGTGTTGGTCACTAACACCCGCTTTTTCGGATTGACCGAAATACCCTTAAGCATTTGTTCGAGGTTATCAGCATCGGCTTGGTCTAAAGGAGTTGGTAACGCACCGCGACCAGAGGAACCAACAATTCCAGTAATACCAGGGAAGTTACGGATAATGCGTCATGCATCTTCACTGTAGATCATCTTAATGTAGATGTAACGACCTAGTAAATTCTTCTCGCTAATCTTCACCTTTAAATAACGGTCTTCATCTAACACAAATCACTTTGTAAAAGCGGTGTTCTTTAATGAACGGGGCAGCTTGCCGGATTTTAAGGAAAAGACTTCTTCGATGACTTCGCGTTCTTTCAAAACCCTTACATCTAAGATTTCGTTGTCAAATCCCAAAGCTTTTACCTTAGCTTTGAGGTTTCGCACAACTGCTTCATCCTTAACACTTACAGGGGCAACGTATCATTGCGGGATTAATTCCACTTGTTCCATAAACTAATTATTTACCTTTAAAAACTCCAAGCGCAATAAAGAGGTGGTTAATGCCAAAGTAGATCCCTATCATAATGGCACTAACTAAAACAATTAACAAAAAGATCGTAATTAATTTCTTGCTTGTTACCCAGACAATCTTGTTAACCTCTTTGTCAATCCCAAACCACAGGTTCGAAAACTTCTTGGTAAAAGGCACCTTAACCTTTGGGGTTTGGTCCACTTTTTTATCTGTGTCTTTAGTTTTACTGTCTTGTTTCTCTTTGGCCTTCTTGGCTTCTATTTTGGCCTTTAGTTGACGGTGTAACTCGTGGATCTTCTCATCGTTGTAGGCGGTGAGTTTTTCCTTGTTCTTTAAACCAAAAGGGAGTTTCTTTTTTTCCATGAAGCTTAAAAGGAATCGAGGTGCTTGGTTTTTTGGTTGCAGTGCTTGCAATACTTATTAATTATAAGACGATTAAGAACCTGCTTCGACCAAGACATTACATAGTTTCTACTTAAACAGTCTTGGCAAACAAAGATAATCTTCTTGCGCACGTGGAGTTGGGTCAATTATATATTATCGGTTTGGGTTACCAATTTAATCTTATGGTAACAGTAAAGTTGGAGCAAACCAAAGGGCAGAAAACGTGATCATTTCAAGGCTTTAGCAAACTTTTGCTCGACGTTTTCGGCAACCTCGTTAATAATGTTGGCTGCCATATTGGACATAACTAATTGGGCCACCATTAAAAGGATTTCTAACACCCCTAAAACAATTAAGACTGTGTTGAGGTTCCGAAAAATCGGATCACGACGTGGTGCACTCAATGAAGTTCTGTTAATGAGGTCATACATAATGCCAATGCCAATTAGGGAGATAGTGCACACAAAGAAGAAGATGCACACCAGCATCGTTAGTTCATTGAGCAGATTGAGCTTCTTAATCACTTTCATGGAATAAGGTGTAGAGCTTATTTGATTTTAATTGCAATCGTTTAGCTAAATAATGACAAGCATCCTTAACCTTAACGCCCATTTGGACTAATTGGGTAATTTCTTGTTTTAAGAGTTGGTCGGAATATTGCCCCACCGGTTGGGTGTGGTGGTTGTTAATCACAATCACAAACTCGCCCTTTAGAGTAATGTCTGGCAGCGGTGCACTTGTATTAAACCAGTAGTGGCTTTCGTGGAGCTTGGTCAGTTCCCGCCCTACAAAAACTTCAGTATTACTAAACACCGTTTGGATTACTTCCAGTGTTTGTTGTAAACGGTGTACCGCTTCAAATAAAACAATGGTGCCTTGGAAGTTTTGGTAGTGTTTGAGTGTTTGGGTTATTTGAGTGGGTTTGTGGTCCAAGAAGCCCAAGAACAGTAGTGGACTTTCGGGGAAGCCACTGGTTACCAAACCACAAACCAGCGCACTAGGACCATTAATTACTTCTATTGCGATTTGGGGTAGTTGCGTTCTGACATACTCCACCAATATGTGCCCGGGATCGGATACTGAGGGATAACCCGCATCACTGACCAAACCACAGTGATACTGGTGGATTAAGTTAGCTACCTTACTTAAGTTTTGCTTTTCCTTAAACTCGTTGTTAATAATGAACTGTTTATTTGAAAAGTCAATGCCGAGTAAGCCCAAGAGTTTTTTGGTTACTCTGGTGTCCTCACAAAAGAGCACTTCACACTTGGTTAAAGCCGCTTTAACTCGCGGACTAACTTCCTCAATGTTGCCAATGGGGGTGGCAATTACTTTAAGAGCTGGCATACTTGGTCAAAGGTAAGTTGGAGTAAATTAAGTTTGCGAAAGAGCTGCTTGTGGTTAAAGTAGCTTAAGTGTAGTTTGTTACACAACGCTAACCGTTTACTTTTACTGTTGAGGTTTAATTCCAAATATTGTTCCCAGCTTAAAGTGGGTTGGGTTGTTTTAGTGAACACCTGACGTTGTTGTAACGCTTGGATTAAAGCTGCATCAGTTGCTTCCGCTACCCCAATTTTAGTGCTGTTGGGTTTCATGTCTTTCCGAGCTATAAAACAACTTTCATACTTGTCTAAGTGTTGTTCTAACTGCCGGCGAATTTTCTGACCAGCAACATCGGGGTCCAAGAAGAGGATCACCGGTTGTTTTTTAGCTACAGCCTTAATCAGGTTAATGGTTCTTTGGTTTAAAGCTGAACCGTTCGTGGTAATAACACTAACATCAAACAGCTGTTGTAAGCGAGCTTGGTCAGTTTTGCCCTCACACACTATCACCCCATCTAGTTTTAGACGTGTTTTTTTATCCATTCAATGTGGCGCGTATAAATGGCAGAAATGGAAGAGTTATTAGCGTAACACAATAACACCTCTTCAAATAACGGTGCTAGATCAATTACCTGAAACTGGGGACACTGGTCAAACTTAGTTTGAGGAATGGAGTTGGAGACAAACAAATAGTCCACTAAGCCTTCGTTAAAGGCCTTTTGGAAGCGTTGCGGTGCTTCACCGTTAAACAAACCATGGGTGGCCATGACACAGACCTTTTTGGCATGGTGTTCCCGGAGTAGTTTAGCTGCTGCTATTACCGTACCACCGGTGTCAATCATGTCGTCCACTATTAGACAGTTTTTGTTCGCTACTTCACCCAAGACATTAATTGATTCCGCTACATTGTGGGCTGGGCGACGCTTGTCAATAATCGCTAGCGGTAGTTCCAAAGATGTTGCAATTAACCTTGCCCGCTTTACACCGCCATAATCGGGGGACACCACCACAAGGTCTTTTTTACCTAACAGCTCTACAGTGCGGGTTAAAAAGACGTGGTAGGTTTGCAATGTATCCACGGGTATATTAAAGAAACCCTGGGTTTGGTCGCTGTGGATGTCGGTTAAAGCTACGCGGCTCACCCCAGCCGTGGTTAACAAATCCGCTACTAATTTACTAGTTATCGGTTCGCGTCCCATTGTTTTGCGATCTTGCCGTGCATAACCATAGTACGGCAAAATGGCGGTAATACTTTTTGCACTACCCCGTTTTAAGGCATCAATTGCTATTAACAATTCCATTAAGGAATCGTTGACTGGCGCACAGGTAGACTGAAAGATAAAAACATCCTTATTGCGCACAGACTCCTCAAAGCGGATGTAGGTCTCCCCATCAGCAAACTCATTATGGGTAATTAACCCCATGGGCATTTTGAGTTTCTGACAAATACGACTAACTAAGTCGTGGGTTTTCGATAAGCTAAAAACAACGTGGTTATGGCGATCCATATTGTTGGCGCAATTCGCGCTGGATCGTTTTACTTTTGATGGCTTCACGTTTATCAAATTTTTTCTTAGGTTTGGCCAACCAGATTTCCATTTTAATTTTGCCATTTTTAAAGAACACTTTAAGTGGAATAATGGAAAGTCCTTCTTGCTTTTGGCGTGCCGTAATTTGTTTAATCTCATTCCGGTTTAAAAGGAGCTTTTTCACCCGTTTGAAGGGGTGGTTGAACTCGCCTGAAAAGGCATAGGGCGAAATGCTAAAATCTTTAATATAGACCTCGTGCTTGTCAATAAAAACATAGGCTTCCTTTAAGCTACCTTGCCCTAAAGCTAGCGATTTGACTTCACTACCTTTTAAGACTAAGCCAGCACAATAACCCGTTAAAAGGTAATAGTCATATTGCGCTCTGGGATTGTTGACAAGTACTCGCATCTGTAGATGAAAATCTCTGTTATTATCTCCACTTACAACTGTGGTGCTTTAATTGTGAAAGCACTATGTTCATTAGTGTCAAACCAAACACCAGCATGTGAGTTAGAAGTGCTTGTGATTGATGATGGATCCATTGATAATACTAGGCAAATTATCAAAAAATTTCAAGCAAAAGTTAGCTTTACTTTAAAGTACTTTTACAAAAAAAACGGAAATTGGGGTAGTGTAATTAACTATGTCAAGGAAAACCGTTTGGCCAACGGCGATTGGATTACTGTTTTAGACAGTGATGATACCTTAAAACCTAATACACTCAACAAGTTGGCTAATTTAGTGGAAAAAGCGGATTATGACTTAGTAGTTTTTGACTACACTAAGTGCTGAAAAAAGATTAAACTGAAAATCCACACTTACCCGACTTGGTGAAAAAACATGACCCGGGAACTACAAAAGCAAACACCGTTTTGTATTCCTCTAGGTAAGTTTTTGAAACGCAACCTGTTTTACAAGTTGCCCAAGCTCAAAGAAAAGGTGAGTTTTCAGGATGCCTTGTATACGGCTAGTAGTTTAAAGCTCGCTAAAAAAGTGCGTCATGTTAATCAGTCTGGTGGCAACTACCACTTTAAGCGTGCTGGTAATTCAATGAGTATTCCCTGAAACATTAAGCGCTTTAGTGCTGAATTGGACATTTGTAAAGACTTAATTCGTTTAAATGCTCAGGAAATTGCCCTAGTGCACTTATTGCGACAACAGTTTCGGGTGCAACTTAAAGAAAAGCAGATACAACTAGCGGTTACCCGTGACTTTAACTTTAGTGGGTTCTCTTGGTACACCCGCTGCTTTTTATGGATGGTATACCAAACGATGTTAAAGCGCTACTTTTACTTACAAACAACTAAACAATAAAAAAGGCTGTGCAGCCAAACCACACAACCCTTTTTATTTTCTTTACTTTAACTATTTCAACAAGTCCGCGTAACGGTTCTTTTCCTTACTTGCCACCAAGAACTGTCACTGGTTTAACTTCCACTTTTTGGTCATTTCACTTACCAGTTCATCGCCATTCTTAAGATACGAAGATGGCAGTGTTACCACAATCGCAATGGCTGCTAACAACAAACCAGCAAACAGCGCCACACCAGCAATTGCCCCTGGGAGGATGCTTACTCCTTTCATTCCAGGGTTTGCTAGTTTCGGTGCTTCTAAGAACATTGAGTTAGTAATGTCAAAGGCGGTGTAGATCACATAACCAAAGCCCCAGATGTAACCAAACAACACGGACACTTCACTACGCTTGTACTCTTTGAATTCGTGCGGGAGGATCAAAATGGTACTTTGCATACTCCAAGCAAACGAACCACCAATAAAGGTTGCAATCGCCACAAAGGCAAAGCCAACAGCAGTACCCACACCTAGAGTGGCTGCAAACAATACGATTACAATCACCATTAAGGCATTCATGAAGAACATGAAGCTCAATCAACGCTTGCGATCGTAAACCGTTTTGTTAAATGGTGAAAACACCATGTAACCCATACCATAACCTAATACCCAGAGGATTGCTAAGGTTGGGAGACCGGCAAAGTAACCACCAGCACCCTCTAGTGGTTTTAATGTTTTAACACCATCTTTAACATTAAACGAACTCGCTGGACTCACTGCTTGGAGAATGTTTCATCAACCACCAGTAAGTGGGTTTACTAACACCACCAAACAGATGCCATACATCCCGATCATCTTTCAGGTCGCTTTTCGCTTTAAGATATCACCAGCACTTGGTTGGTTACCCAACTGCATTTGTTCCTTGGTTTGCTTTTGGGGCATAAACGGATCCATTTGCTTTTCAAACCAAGCAAACACACAGAGAATGCCAAAACCAATCAAGATTAAAGCAGTTAGGATAAAGACCCAGTACTTAGTACCAGCTTGTTGAACAGATTGGAATAAGAACGGTACAAAAGCAACTACAATCCCACTGTTAAAGCCCCAGAGGTTCGCGTTGGATAACACTGACTTACGACGTTGCGTTGAAAGTGAAGCAATTAACGGTTGGGAGTAAGTTACTAAAGTTGTACCACCAATAGCAATCGTGGAACGGAAAATAATAAACAACGCATAACCAGCAATTGATGAACTGGTACCAATTAAATTTTTATCTAAACCTTGAACTAAATCAATAGTTTGACCATCTGCTAACATGGTCTTGCCTTCTGCCATAACAGGCCCCAACAATTGACCTTCATGTAATTGCGAAGATAGACTAGAAAGTTTACCCATTACTTCCTTTGAAAGTGGTCGCAATAAAGTTAACTGGTTATTTCCCCCTAATGGATCACCAATAATTAAGAAGGGGAAACAGAGACACATTAGCCCCATCATAATCATGACCGCATAACGGTAACCAAACTTCAGGACCACTATTCCACACAATACGGATCCGACTGCACGTAGCAGGGTAATACTCCAGTTGGTGGCACTACCAGCAATTGATCCAGCACTGTTAGCAAAGAAGGAATTGACCCAACCATCATAGTTAGGTAAAACCTTAGTATTGCTATCTAAAATACCAGCTGGCTTACCAGCAATTCGGTCAATTACAAATCACTCTACGACAAACAACAAATAACCAAGCAAGACAATTGCCCACAACATGACAAGGCGTAAATCACTCGGCTTTTTTACAGACGTTTGCACAATCTCAACGACTCCTTAGTTAATTTATTTTTAAATTGTTTCAAGTCGACTTTTGTCTCTTATCTAAAAACAAGCTAGTTTTTTAGGCTTATCTTTGTTAACAAAATAAAAGACCAGAGCCGTTTGGCCCTGGACTTTTATTCCTTTTAGCGGTTTATCTAAGTTATTTTAAGAGATCCGCGTAACGGTTTTTAGCTTTATTCGCAATCACAAACTGTCACTGCGTTATTCTCCACTTCTTTGTCATTTCACTTACACATTCATCACCGTTTTTAATTCAGGATGATGGGAGGTAAATAATGACCCAGTTAATAACTAGAAGGAGTGATACAAACAAGACAATAGCGGCAATGGCACCTGGCAGAATATTGGCACTTGTATTACCAGTAGCAATTCTTGGTGCATCTAAAAAGACAGACGTAATAATGTCAAAGATGCTGTAGAAGACGTAAGCAAAGCCTCACACATAACCAAAGATAATAGAAACTTCGTTGCGCTTGTATTCCTTGTACTCGTAAGGGAGAATCAAGATCGAGCTACCTAAACTTCAACCAAAACCACCAGCAATAAAGGTAAAGATACCCACAAACGTAAAGCCAACCACAGTATTTAAACCAAGCGTTGCAGCAAAGATTACTATTAACAACACCAACACGGCGTTGGCCGTAAAGATAAAGTGGAGCCAACGCTTCTTGTCATAAATTGTTTTGTTAAACGGTGACAGTAAGAAGTAACCCAAGAGGTAACCCATTACCCAGAGAATTAATAAGATTGGACGGGCAGTTGGAATAATTGCTTTTTTAGCACCTGAAATAGCTAACAAGCTGTTAAACCAGGTTTGGGTTAACGGGTTAATTAAGAGAATTGTACCTACACCAGCTATTGCCAACAGCTTCCAAGTTGTTTTGCGTTTCAAAATTGATAACGCACTTGGTCTCACACTCAAGCTTTGATTGGTTTTTGATTGCTTTTGGGGCAACATGTGATCGACCTTCTTTTCAAATCAAGCAAATACTAACAACATCGCAAACACCAATAAGATCATGAACGTTACAACTCAAACCCAGTTAGCAGTCGCAAAGCGGCCAACACCTGGAATTAAGAAAGGTGCTGCCACAATTACAAGTCCTACGTTAAAACCTCAGAAGTTAGCATTCGATAAAATCGATTTCTTGCGGTTTGAAGAAAGGGAAGCAATAATTGGTTGCGCATAAGCAATTAAGGTCGTACCACCAATAGCAATCGTAGAACGGAAAATAATGAAAAACGCATAACCAGTCATTGACGCACTGGTACCAATTTCGTTGCCGTTAATTCCTTTAATTAAGCTAACAGGAGTGCCGTCAGCCAACATAGTTTGACTACCCACTTGAACAGGACCTAACAGTTGACCTTGTTGTAAACTGGAAGAAATTTTAGTAAGTTTACTTATTACTTCACTTGAAGCCGGTCGCAACAAGGTCAACTCGTTATGACCACCTAACGGACTACCAATAATTAAGAAAGGAAAACAAACACACATGAGTCCCATCATGATCATCACTGCATGACGGTAACCAAACTTCACCACCATTACCCCACATAAGATCGAACCAACTGCCCTCAATAAGGTAATAGTTCAGTTAGTAGCTTGACCTGGAATAAAACCAGCGTCCGCTACAAAAAAAGAACTGAACCAACCACCGTACTGGGGAACAGCACTAGTTTGTTCAGTTAAAAGGGAAGCCGCACTACCTCTAATGAAGTCAATCACAAACCATTCAATCACAAACAACACATAACCAAGTAAGACGAGACCTCACAGTGTAACGAGCCTCAGGTCGCTTGGTTTTTTTACAGAATTTTGCACTTCAGTAATAAAAGAAAACGCAAAAATTTTTATAAATTGTTTGCAACAGCAAATTCCGGAATTAAGCGAGCTCTTCTAAAAATTCCGTTTCTTTCTTGTTAACAAAAAGCGCAATAAAAAAGCAGACCACTGATGGTCTGCTTACAATTTATATTGAGAAATTTTTATGGCGGAAGCGGTGGGATTCGAACCCACGCACCGTAGAACGATCTAACACCTTAGCAGGGTGTCCTCTTAACCACTTGAGTACGCTCCCAGGAACTGGAATCTAAATTTATTATAAATATTGGATTTTCTGACTTTAGGATGTTCAAGCCATTGTTAAGCGCTGAGCTGTTTTTTAACTGAACAGCTAAAGATTTTAAAGATAAAACAAGCTTTTTAAAACAAGCGTGTCGTGTTTTACAGGCCAAAAACTGCATTAAAGAAGAACAAATAGCACTCACTGCTTTAAAAGAAAGGGAGGCACAAATCACCACCGGAATTATGTCAAAACTGGCATTGCCCCACATGCAAAGTGCTACCGTGTTAAAACCGTTTGTGGCGGTGTTTAAGGTCAACAATGTGGACTGGCAATCCTTAGACAACCAACCGGTTAAACTAATCTTTTTAATTGGCGTACCCAAAGATCAAGGCAACCTCCACTTGGAATTTATTTCGCAGTTTTCCAAGTTAATGTTGCAGGACGAGTTTGCCAACAAAGTGCCCAACATTCGCAGCTTTAACGGGTTGATCAATTTAATTGACAGTTTTCAACAAACTGCAGTTGCAAGTCAACCTGTTGTTAACGAAGCAGCAGCTCAGACTGAAGAGCCAAAGGACACAAACACTCAGTATGACTTTGTGGCGGTAACGGCGTGTCCCACCGGGATTGCTCACACCTTTATGGCCAAGGAAGCGCTGGAAAAGTTTGCCCGCGATCACAACCTCAAAGTTAAGGTCGAAACTCAAGGAACTGATGGGATTCAGAACCAGTTAACCGAGTCTGATTTAAACAATACCAAAGGCATTATTTTGGCCTGTGACCGCTTAATTGATTTAACGCGTTTTTACGGTCACGCAAATGTGGTGGAAGTGTCCACAACCAAAGCAATTAAAACACCACAAACAGTTTATGACCAAGTGGTAAAGAAGGAAGGCAAACTACTCGGTAATAAGTCCAGTGATAGTGCTAGTCAAACGGAACTCAAAGAAACCACTGAACAGCTCTCGTTTAAAGACTTTCACAAACGGATTTACCGCGCCATTTTAAGTGGGGTTTCTTACATGCTGCCATTTGTCGTGTTTGGTGGCATCTTAATTGCAATTGCCTTTTTAATTGACATTAATAATGCTGGCAATGCTGGTAAACAATTTGGCTCTAAAGATCCCATAGCTAACTGGTTTAAAACACTCGGTGGGTTGTCGTTTGGTTTAATTGTTCCCATTTTAAGTGCTTACATTGCCTTTGCTCTAGTCGGCCGGCAAGGGTTACTGCCCGGCTTTATTGTGGGGTTAATTTCAGCTGGTAAGTTTTTACTCAACATTGACATTGTTACTGGAAAAATTGATTGAGCGACTGAATCAAAAGTATCGAGTGGTTTCTTTGGTGCCATTTTTGGAGGCTTGTTGGCAGCAGTGTTAATTATTGTGCAGCAACGTTACATCTATAGAAAATTACCGCAAGCATTACAAGGCATTAAGAACATTCTCTTTATTCCGTTACTAGGAACGCTAGTCACAGCAGCCTTGTTCTGAGTCATTAACATTCCCCTAATTTACTTGAACTATGGTCTGTCCAAGTTCTTGCAAATTATGGACAAACCATATTTGGCTCCCTTACTTGGTTTAGTGATTGGACTCATGATGTGCTTTGACTTGGGTGGACCGGTCAACAAAGCGGCCTATGTGTTTGGGGTAGTTTCGCTAGAGAGCCAAAACAGTGGTACGGTTGCAATGGCTAGTGCTATCTTATCGGGGATGGTGCCACCATTGGGTATTGCCATTGCTGCGACCATTCGCAAGCAGTGCTTTGACAAGGAAGAATTACCAGCAGCGTATGCTTGTTATGTGATGGGATTAAGTTTTATTAGTGAAGGGGCGATTCCTTTTGTTGCTAAACGGCCTAAGATTATGTTAGCTGCTAACCTAATTGGTGGTGCTGTGTGTGGTGTTTTAACGGGAGCATTTGCGCTAACGATTCGTGCCCCTCATGGTGGCGTGTTTGTCTTTGCCTTATTGAAAACTAACTTAGAGGGCATTGCCGGCAACACACTCCAAATCGGCGCTGGGGTTGGCTTAGCACTTCTAGCGCTAATAGTATCCAGCTTCATTAGTGCTGGCATTATTATTGGTCATAACCTGCTTGTAGTGCGTAAAAAAACAAAACAACTAGTAAATACCAATGCTTAACCACAACAGTAAAGTTTGAATAGTTAACTATGCTTGTGCGATTGACTATTACTTAGATAAACACAAACAACAACGCGGTGTGTTAACACCCGGTGGTAAGGGAATTAACATGGCGATTGTAATGGCTTTATTTGGCATCAAACCAACCGTGTTAACCTTTTTGGGTCAACCTACTAAAGATTTATTCTTGCAGTTGTTAAAACCGTACCAACTGGACTTAGTTAGCTTTCCAGCGACTACCCAAACACGGATTAACGTGAAGCTGTTGGATGGTGCCCAAACAACTGAAATTAACGATGTCACCCCGTTAATGGAGGAACAAGCAGTCCATGAAATGATTGCTTACCTTAAGGCTAACGTTAAGCCCAATGACCTGTTGGTGTTGAACGGCCGTTTTTTACAACGCGATTTAGTTAAGTTGTTAGATGTTGCTTTTAGCTTAACCAAGTATGTTGTATTAGATGTTGATGAACCTCAACTATTACAACTGTTAAACCAGCGCCAACCCTGGTTAATGAAACCGAACCGTGATGAGTTTGTAGCAATGGTTAATGCAAATAACAGTAACGTTGACCAACAAGAGTTGGTGCAGTTAATAAAGCAGTTTCAGACTACACAGAACTTATTAATGTCAGACGGCGCGCAGGGTGCTTACTTCTTTGATCAACAGCAACTGTTATTTATGGAAGCGATTCCTCCCCAACAGTTAGTTTCCACTACGGGAGCGGGAGATACATTGCTGGGTGTTTTCCTAGCTAACTTACTGTTAGATAAGGATCCAGTGGGTAGTTTAAAGGTAGCGGTTAACTATGCTAGTGCTACGATTAGCAAGCTCGGTGTGGTCAACAGTAATGACCAAATAGTGCTAAAAGCTACTAATTATTACTATTTATAATTAGTAGATGTCAACTAAAGAACTAGCCAAACAGCCAAAGGTCAATCAAGAGAGGGAATCGATTTTGTTACCCGTTTTGCTAGCTGTTTTCAGTATGCTCTTCTTTATGCTACTGTTAGCTTTAGTGTTGTTGTACGCTATTCCAGGTAAAGTTAATAACTTGAACTAGCCACAGGTTTAACTGTGTTCAATTGGGAAGCCAAACTTAATCTCGCACTTTTAATGAACTAGTTTTTGTGCGCAACTTAATTAAAAACGTTTTTCGCTCTTTTCGCACCGCTAAGATTGCCTTAATTGCGCTTACCTTTTTAATCTTTGTCGCGGTTGGTAGTTTCGTGTTATTAAACAATACCGTTAATAACTTTAACGCTTCCTTTCAACACGTTACCGAACAAGGACAATTGTCTAACGCCATTATTAACGAGCGCTATGACTTTGGTAAACTCGAGTTCCAAAAAACGAGTAATGATGCTACTAGTTCTGCCCAACCTAGTGTTAGTACTCGAAGTACTTTAACTGCCCAAAAAGCAAATGGCGCTAATTCCAGTTTTACTCTGACCTTAACACCTGACTCACGGACTTCATTTATTAACAACGCACTGCAACTAAAACCGGATGTTTATAACAGTTTAGTTACCGTTACTTTTAGTTATGGCAGTGAATCTGATCAAACCAAGAAAACCCAAATAGAAGAACAGTCCAAGTTAATTGCCGCAAACAATTTAAGCCGGGCATTACAAACCGATAAGGAGTTATTAGTTACGGGACAGTTAGAACAGTTAAAGGCTACCTTTCGCACTTATAAGGCAATTAACATTACTGATCAAGGAGTGTTCAAGAAGCTCATAGCCTCTGAACCGACCGATCAAGTTAACCGTCTCGTTCTGTTTGACGGACATCAACTAGCACGTTCCCGCCAAGTCGAATTTAACGAGCTGGTTAGTCAGTTTACCCAGGTGCAAGCTAAGGGTAAAGATCAGTTATCCACTACTCTAAAGAATGGTAATTACGAACAACTTTTGCAAGCGATCGCTAACAACTTTGGTAGTAGTGAAACTGAGTTACTTAAAGAAATTCAAGATGCTTTAAAGCAAACGAATAGTTCCACCACCCTTGCCCAAACCCAAAGTAGTCAACAATTTAGTAAGTTAAAAAACCTGTTTGATTCTGGTAGTACCTTTACTAACATTGCCGGCAAACTCACGTTGCAGTGGATGGAAGGTACAAACAAAAAGCAACTGGTAATCTTTGACCCGAGTAGCTATGAAACGGTAGTAGCACCGGGTAACTGGAGTTACGAACAGCAACAGGGCAAAGCGGTGTATCAGGACATTAACCACTGAAACCGGATTAAGCGTTTACCCTTCAATGAGTTTGAACGCGAATTCCAAAAGATTGATAAACGGTACAAAATGTTGGTCGACAAAATTGAGTACCTCATCTTGGGAGTGGGAATTACACCAGACTTTGTCTACCCCGTCTTTAGTGAATCACTGTTAATTCCCAATACGGCTAACGAGCAGTTGTATTATGTCAACCAAACTGGTTATGAACGTACCGCTAGCTCCTTTTTAACTAATCCCACCGAAACTGGCATAGTAGCACGGTTACCTAACCTCAACCATGACCTTGATGTAATTAACCAGTGGGCTGTTAAAAACATGTCCTGACCCCCGAACCTCAAGGCCGCTTACAGTAATACCGATACCACTAACATCCTTAACCTGTTAGCAGCGCGAACCGTTTTTATTCCTAATCTCATTAACACGATTAGCTTAGTCTCTTCCTTCTTAACAATTGCCATCTTACTAGTGGCCATCTTTGTCACCATTTTAATCTTGGTTAGTTACCTCAAAAAGAACACGGAACAAATCGGCATCTTAAAGGCTAATGGGGTGAGTGGCCGGAAGATTAACTTAAGCCTGTTAATCTTTAGTTTAATCCCGGGGATAGTGGGGTCCTTTATTGGTTATACGTTAGGTATTAGTATGCAAAACGTGGCCATTAACCTCTTTAGTAACTACTGGTTTATCCCCACGCAAACTTCGAGTTTCTCGGTGTTAGGGTTACTCTTCTTTGCCTTATTAGTGGTTCTTATGATGAGTGGGATTAGTCTGTTTGTGGGGTGACTGATCTTAAGGCAGGATGTAGTCAAGATTCTTAAACACGACAGTGAGTTTAAGGTATCGCACTTAGGGTTGTGAGCTAAATGACTGTTTGCTAAGTTTGGTATTATGACCAGATTTCGTGTCGCTTTAGCCTTTAATGCGCCGTGGAAACTGGTCTTTTTAACCTTAATGAGTGCACTAACGATGATGATCCTAAACCTTAGTTTTGCCACTAAGGATGCCTTTCAGGTAGCACAAACAAAGACTACTTTAACCCAATCGAACCACCAGTACGAGTTTGAACTAGCATCGCCAACGGAGCAGAGTGGCTTGCTCAAGTGACAGCTGTTTGCCGAATTGGGCGCTACGGATGATCGTACTACCGATGCCGTTAAATTAGCAAACCGAAGGATGGAAATTCAAAGCGTTGACGAATCCAAAAAGTGAAAAAACCAACAGGTAATTAACTTTGTTACTGATGCCACCGGTTTTAGTAATGACATTAACTATTTAGAAAACATTGTTCAGTCCAAAATTGGCTTGGACTATTCACTGGGTTTTGGTAACGTTTCTTCCAATCCGTGGAAGCTGAGTGAAACATTAATGCCCGCCAATCAAGCATCCGCTTCCAACACGGCTTTCCAAAACTTTTTAAAGGCGATTATTGTAATTGACCCTAACGAGGGCGGTAAGTACATTAAACAAACTAAGGATGAAGTTACTGTGCGTTTTTTATACGAAATAGACAGTAGTAAAGCTTTAAAAAACGGCAACACCAAATCAGTTAATAAAATACAAAAAAGTACCGCAGCACCCCAAGCCCAACAAGGACAAAGTAACCAGTTAATGCTTGACAATGATTTTGCTAAATTCTTGTACAAGCAGTTTAACCTAATCAAGGAAGGGGGCAAAGCAGATCCGGTTGATCTCAATAACTTTGACTTTGCTAATCAGAATCAGATTAAGCGTTTTTACAGTAAATACAACGCCTTACCACCGGTAGATTACAAGCTGGCCTTTAACGTGATCGGTCTCCCTAAAGAAACAATTGCCAACCAAAAGGATGCGCCCCTGTTTGGCTTTTTAACCTTGCAAGCGCAACTGGGCAATGACGCGATTAAGATTAAGGGGATTAAAGACTGAAAGCTGGATAACACCAACTTGGGTCCTGTGTTGCGTACCAATGAGGACGAGATTATTAACCAGCGCTTGTTTAGTAAACCCAACGGTATTATTATCAACAGTTCAGCTGCTAAAAAGTACCGCTTAAAGGAAGGTAGTCAGCTCCATGTCAAAATTCACAACGGTTACAAACGAGTGAATGCTAAGTTAGTTAACCAAGATCCCACTTTAACTGCCACCTTTAACATTGTTGGCATTAACAATTCGGTGCACGAACCGGAGTTTTACACGAGTTACAAAACGGCAGCAACACTATTGCAATACCCCAATGAATGGATCGAAAAGCAGTTACCCTTTAACAGCTTTTATGCCAATTCGATCTTGCCCTTTACCCAGTCTACTTCCCTCTTTTCCGAATCTGGCTTATTCCCTGGTACCAGTAGCTTTGCCGCTAACAACACGGTGCTTACTGAAGTAATTAAGAAAACGATTGAAGGTGGTAAAACTAACAGTAAAACTGATGTGCAAAAGCGCGCTGCTCAAGCTACTGCTAACGGTAAGGATAACAGTAGTCAAAACAACAAGATGAATTATGAGCGCTTACAAAAGGCACTGGGCATTACCAGTGATTTAGATAGCTCTAAAGCCGGTGAATACGCCACCATCTTAGAGCGGGTGTATGATGGTTTGCCATACAACTCCACTATTAGCTTTATTAGTAATGTGCAGGCCAACGATGCCTTGTTTGCTAACATTGCTAACACCACCCAGCAAATCCAAACAGCGGCCATTGGCATTATTATTCCGATTATTATGCTAATTGTGTTGTTAGTATCAACTACCCTAATTCAGGAATTGAAGAAGATTGCGATTCGTTTAAAAGCATTGGGCTATTCCAACCCGAAGATCTTGCTGTCCTTCTTGTCGATTTACCTCCCGTTATTTATCTTTGGTTTGTTAATTTCGACCCCAATTTCGATTTATCTAATTGCACTCCACAATCAAGTTATTTTCTCCAGTGCTGCCATCTTACTGGAAGCCACATTAAACTTCCCCACGGTCATTCTGTCGATGTTAGTGTTAAGTGGGGTGTTATCAATTACCTTTGTGTTGAATTGGTTGGAGTTGAACAAAATCAAAATTGCCAAGGAGATTAAGAATAGCTAATGCGATACTTTGCCCTCACTGATTTAACCAAGCCCACTGCAAAATTTACCACTGATGCCATGGAACTGCGTCAGATATCCAATGCTTACCTCAACCAAGCCCAAGCATATTTGCAAAAGGGTTTAAAGCAACTCAAAAAGGATTACAAGAATGCCATTTTGTATACCCCCAAAACGGAGTACAAGCGCTTTTTGAAATGGAAGCAAACTTTTCTTCAAGATTTAAACCAAACCCAAAAGCGCTTCTTTATTGTTCGTGCCCAACATTTTTCTTACGTGTTGTTGTTTAACTTATTGGATGAACAGGTGGAAGCGGTGATTGCGACCTTTAACAACTTCTTGGATGAACACCGCTTGGAGGCACAATCCAAGCAGTTTGATTTAGACACAGCTGTCAATGAACTGCATGATTACTTTGACCAACTGCAAAAGAACACCGCTTACAGCGAGGATTTACCAACTCACCTCACGCAAAAAACCGAGCAATTAATTAACGCTCGTAATACCCAGTTAACGAATTTGTTAAACAAGATTGCTACTACCAAACCACCCCTAAACAAACAGCAACGTTTGTTAGCCAGTTTTCGCAATTACCACGAACACCTCTTTTTAAAGAACGAGGTCAAAAAGGTGACTTGGTTAAACGAACCACGCGCCAAAAAAGAGTCTGTTACCCCTGATGAAGAACACATTATTGAACTTAAAAACGTTTACAAGTACATTACTAATGGGGTGACTACCAATGCCGTTTTAAAGGGCATAGATCTCAAACTCAAAGCCCATGATTTCATTGTGATTTTAGGTCCCTCTGGCTCGGGGAAAACGACCCTGCTCAACATTATTTCCGGGATGGACCGGCCATCAAGCGGATCTGTTGTCGTTAATGGTCAGGAAATGATTTGCATGAACGACCGTCAGTTAACTAACTTTCGCCGCAACTATGTCGGTTACATCTTCCAGCAGTACGGGCTTTTACCCAACCTAACTGTAAGGGAAAACGTTGAAGTGGGTGCTAACTTGCAACGTAACCCTGATAAGCGGATTAACATTGATGAACTGTTAGAAGCAGTAGGGATGAAACACCTCCAAAAGAAACTCCCCAACGAGTTGAGTGGTGGACAACAACAACGGGTCTCGATTGCACGGGCGTTTGCTAAAAATCCCTTACTCATTTTTGGTGATGAACCAACTGGGGCTTTGGATTTGGAAATGACCCAAATTGTGCTCAAACAGTTTTTAGCCATTAAACAACGCTATAAAACCACGATGGTCATAGTGACGCACAATAACCTAATTGCCCAACTAGCCGATTTAGTCATTTATGTGGCAGATGGCAAAATTCAAGCACTGCAAGCAAACCCTAATCCCAAACAGGTTGAGGACATTAACTGAATTTAGATCTAATAAAATTAGAGCAAATGAAAAACCTGTTTGCTTGCCAACACTTAGCGTTAAGTGCTATTCAACACGCTAAGGGTGGTCATGTGGGAATGGCCTTAGGTGCTAGTCCTATTTTGTACACCTTATGGACTAAACACATCCAGTTTAACCCTAACTGTCCCAAATGGATTAACCGGGACCGTTTAGTGATGAGTGCAGGTCATGGTAGTATGGCCTTATACCCGATCTTACACTTTGCGGGGTTAATTACGAAGCAGGAAATGCTCCATCACAAATACGGGCAAGTCAACACCTCTTCCCATCCCGAGTATGCCCCTAATAACTTTATTGATGCGTCCACTGGTCCCTTAGGACAAGGCCTAGGAATGGCTGTCGGGATGGCCTTGACACAACGGGTACTAGCAGCTGAATTTAAAGCGTTAAGTCCCAAACTGTTTGACCACTTTACCTATGTGGTTGTGGGTGATGGTGATCTCCAGGAAGGGGTGAGTTATGAGGTAGCTCACTTAGCAGGGGTCTACCAGTTAAATAAGCTAATAGTGCTCCATGATTCCAACCGGGTGCAGATGGACAGTGTCGTGCGTGATGTGTCCTTGGAAAACCTGCAAACCCGGTTTACCAACATGGGGTGAAATTACCTTGAAACTAGTGATGCAGTTGCAGACATTGATGCTGCCATTGAACAAGCGAAAAAGTCCGATAAGCCGACCTTCATTGAGGTGCACACGACAATTGCTAAAAACACCACCTTGGAAGACCAACCCGCTGGTCACTGGTTTATTCCTACCGACAAGGACTTTGCGCGCTTTAACAGCAACACCAAGACTAACTTTACCCCGTTTGAATATCCCCAAACGGTGTATGACTTCTTTCACAAGCAGGTCATAGCACGCCAAGCTAAACCAGTGCAAGCTTACAAGGAATTACTGGAAAAACTAAAGGACAAACCGCTGTATACTAAGTTTATTAATTGAACGGAAAACGATTACCAGGCGTTGTACCTCAACCAGTTAGATGAACGCAAGGTAGCACAAACCAATGCGGCAACCCGTAACTACCTCAAGGACTTTTTAGGCCAAATTAACAACAGTAATTCCAACTTGTACTGTCTCAATGCTGATGTAGCACGTTCGTGTAACATTAAGTTAGGTGATGATAATTTACATACCAATCCGCATTCGCGCAACATTCAGGTGGGTATTCGGGAATTTGGTATGTCCACCATTATGAACGGGATGGCACTCCATGGTGGGGTCAAAGTGATGGGTGGTACCTTCCTAGCGTTTGCTGATTATTCCAAACCCGCAATTCGCTTAGGGGCGTTAATGAACCTCCCCACCTTTTATGTGTACACCCATGACTCTTACCAAGTCGGTGGTGATGGACCAACCCACCAACCGTACGATCAACTCCCGATGTTAAGGGCCATTGAAAACGTTCAGGTTTGACGGCCTTGTGATGAAAAGGAAACAGCGGCTGGGGTTAACTATGGTTTGTTAAGCCAAGACCAAACTAACGTATTAATTTTGACCCGTCAAGCTTTGCCTTCCCTAGAACAATCTGATAGTGTACAGACCTTGAAAGGGGGCTATATTATTAGTAATAGAAAACAACCCGATGTCATTGTGGCCGCTAGTGGCAGTGAAGTTCAGCTGGCATTGCAACTAGAACAAGCTCTAAACGAACAACAATTAAAGACGCGGGTGGTGTCAGTGCCCAACATTAACATGTTGTTAAGTCAACCCCAAAGCTACTTGCAACAACTGTTTGATCCTAACAGTGTTTTACTCACCTTAGAAGCGAGTGCTAGCATGGAGTGGTATGCACTAGCAAAATACGTCAAAAAGCACACCCACTTAGGTGCTTTTAGCTTTGGTGAGTCCAATGACGGGCAAGTCGTGTATGAACACAAGGGCTTTAATGTGACTAACTTACTCAAGTTGATTAAAACTCTAAAGAGTTAAAATAGAGTAATGGTTAAGGTCTGAAAAATTGGGTTTGGGGTCTTTCTACCAACAGCGCTTTTATTCTCGGCCTGTTCGTTTAAGGACTATATTCCTACACCAAGCTTTAGGAAGGATTTCAGTACGGAGAACAATTTTGTTAAAAATAAGGTTCCCGGGAAGGATGACATTTACAGTAAATTTTACGATCTAACCTTTTCCTTAAACTTTGTTAACAACCAAGCCCAAGATTTTGGTACCGGTTGGTTAATTGACTGGAAGGGTGATGAGAATAAGGATCTAAGCAAAAACAAAGAAGGACAAACGGCATCTCAAACCAGAAGTTCCTCTGAACAAACTACCGATCAAGACGCTAACCTCTTTACTGCCTATATCGCCACTAACCTCCATGTCGCGGATGGTTTAAAGAATGATCAGGACTACGCTCCTTACAACAAGGATGGTTGGGGTCAACCATATCCTTACCAGCAAAAAACCCAATCCTTCTTATTAGGTAAGTACACCAAACCTAATGTCCAGTTAGTCAAGACTAACTATGAAAAGCCAGAGGATGCCGTGATTGAGCAGAAGCTCAAGGAAGATTCGCTGTTGTTTATCCAAACGAGTACGTTACCTAAAACAGCATACGCAGCAATTGATCCGGTGAACTTTAGCTATAACCCAACCCGTACCAACGGTTTTTGAACAGCCGGTAAATACAATGTTTACAATGGTGGTAACAGCATTGGTAACTATGCTGATTTTGCTGTGATTGAAGTACCGTTAGTACTATCCAACCCCAATGATGCCAAGATCTACCAAGAGTGGATTAGACCAGCTACCCAAGCCTACAAATATTTAGGTGATGTAGAAGGTTTGTTTGCTAAGAAGGGCTATCGCAGTTACATCCAAGATTTCTACCACTTATTGGGTTATCCGGTAACTAAGAACACTAAATCAGAGTTTATTTTGGGACAAAGTCAAGGTACTGTGAACCACATGAGTTTTAGTAATGATGAGTCCAATACTACTAATACTATTACTAAAGCTAGCTTAACTCAGCAACCCCACAAGGAACAATCAGCTTACGTGGTACGTGAAAGTGGACTGCCAACCCTAACAATGAATGTGGACAAGTACACTGGTGCAAAGGGAACTCACCTAGTTAACGTTGACCAAATTACTGACCTTAGTTTGGGTGATGGTTTAATTGACTTTGGTGGATTATCACGCTTTATCTTGCAGTACCACAATGTGAACTATAAGCAATTTGGTTATGGCACGATCTTGTGAGATACTAACTTTGCCGGTGGTTCTTCTGGTAGTGCCATCTTTAACCAAAACAAGCAGATTAACAGTATTTACTTTGGAGCATTAGTTAATGTAACTACCGACAGAAACGAAAATGTGGGTTTAGGCTTAGGGCAAATTCTCCGTGCTCCCAATACCTTCAACTCTTCCCACGAAGTGCCCTATTCTTATGACCTCATCTTTGGTGATGTTAACACCACCAATTTTTACGCCCAGTTTGCGAAAAAGCACAACACCCACATGTGGAGCAAAATTCAAAGTACGCAGAATGGAGAAATTGGTTTCCACAAAAATTCAAAAACCGGTCAACAGCGGCATTAATCGGTTTTTGAGTAAATTTATAAAGTACTATTTTCTTTAACTAGTTGCCATGTTACTCCGCTCTGTTTGGTATAAGCTTGGTAGTCTGTTAATCATCTTACCGCTAACGGGTTGTGGTTATGTCAGACTAAAGCGTGCTAATTTCCAAACAGACTTTACCATTAACCGCATTCCCACCCAAGGGGATGTGTACCATGATAACTATGACCTGACTTTTTCACTCAACTTTGCTACTAGTTCCAAAGATTCTTATGGTACGGGTTGGTTAATTGACTGGAAGGGTGATGAGAATAAGCCTAGCAATACCGATCCCTTCCTGATTTACTTAGCGACTAACCTACACGTAGTCGATGCCTTGCGCAATCCCCAAGACTATGAACCTTACAATAAGGATAGTAATGGTCAGGACTATGGTAATCGTGATATAACCCATTTCTTTGCATTGGGTAAATACACTGATCCAGGTTTATTGGGTGTTGAAACAAAAGAGCAATCAGCCTTTATTTCGATTCAAACATCAGCAATCCCCAAAACCGCTTATACGGCTAATGATTTTGTTGATTATCAGTATGATAGTCTTACAAAACAGTGAAATAAAAAGAGTGATCAAAAAGATCAAACTCAAGAACAAACGGTAATAGGGCAAAGCTGATCTTACAAACCAGCTTATGCTGACTTTGCGGTAATTGAAGTGCCATTGTTCTTAGACAACGTCCGTGACAAACAGGTCTTTGATTATTTTGTCAAACCAGCCATTGAGACCTATAAAAAGCTTGGTGATACGGCTCAATTATTTACTGAGCAAAACCTAAAGGAATTGGAAAAGGAAACCTATATTATGCTGGGCTATCCGGTAGTGGAGAGCAATATTTATGCCCACATTTTAGGTCAAGGAAAAGAGTTGCGTGTGACTCAACAAGTCAACAACCAACCAGTTAAAAAGAACTATGTTTTGCAAACCATTACTAAAGAGCAACACACTTTGGATATTACGCGTGAAATACCAACCTTAATCCAAAACAAGAGTTTATCGGGTGATTTTGTTGGTAGTAAGCTCTTATCTCAAGAAGAACAACAGCAAGAACATGCTTTTTTGGGTAACTTAAACCAAGGGATAATTGACTTTGCTAGATTGTCAAACTTTAACTTGCAGTACCACAACCGCGAATACCAACAATACGGTAAGGGTTTAGCATTAGCTAACACTAACTTTAGTGGTGGTTCATCCGGTACCTTAGTTTTAAATCAACAAAAACAAATTTCCGGGGTTTACTTTGGCGTGTTAGAGTTTGGTGGCACCAACGGCACTAACCGTGAAAGTAGTATTGGTGTTGGTCAAATTTTGCGAGTAAAAGATGACGCTCAACTCCAACAACACAGTCACAACAACTTACTTTCACAATTAGGTTCCAGTCATAACTCGATTACTTACGACATTATTTTTGGTAACAAAGACACTAAAAACTACTACGCCCAGTTTGCCAAAAAACATCAAACACATTTGTACAGCCAAATAAGTTCAAGTAATCAGCAAGAACTCAAATACGTCGATAACGATCCAAATCTCAAGATTAAAGAAGAGGCTGCCAAAAGCACTGTTCAGAATTTAACTGTCTACAGTTAATTACTTTTCGCATTTCAATAATTGAAGAGTTGGTTAGCACAAATTTAAATTTAAGGCTAGTTTAATGACTATTTATTTTTAATTAGTCAACAGTGATTTAAATTTTATGCAATTAACTACAAGTCAACAAATGCAAACTAACTTTCTTACTACAAATAAACATCACTTATTGAAATACACCAACGGTTTGATTTGGTGTTGGTGATTATTTGTTATTTCTTTAGTTTTAGCTTCATCGACATTTAGAGGTTTCTTTCTCGGCACTATCAATATTGTGAATTTTGTGTTTTGGATATTAGCACTGATTTTTGGTGTAGCTGTCGCTTTCATTAATGGTGTTTTGAGTAGTGAATTAAAAGAAAATTCAGTTTTTCAAGAAGAACAAAAACGATTCTTCTTGGGCTTTTTCTTTCCACAAATGGCTTTCTGTAATGCGCTTTGACTGAAACTTAAATTAAGCTATCTAAATTCTGAAAGAGAAAACTTATTAGAAAAAATAAAGCAAAAACTAAAGAAGCTTACCTTGTCTGTTTTTGTTGTTTGAGGAATTTATTGTGTTTTAGCAACCTCAATTTATTTACCAAATGCATTAAGAATTTTAAACATTTATCAAATACCTAACTTAATCGCTTTAATAAATAATAGATTGAGTGAAATTTTGCCAGATGGTAACCGTTATTTTTTAGGTCATTCTTCATTCGCTTTTCATTACTATGAAATAGTTTCAAGGATTCCTTTTTTAGTTTTCTTTATTATTCCAACAATAACCTTAATTACTTTAGGTTGCTATTTATTCGCTTACTTAAGGTTTATAAACTCAAATAAGTTAAGAAAACCACTTTCTACTTTATCAATAGTTATCATGCTGACTGATGTTGTGGGAATAATTCAATGAATAATTATTGATATCTTATTAATTTGGCTTAATGTACCCTTTGTTATATTTGTAATTTTTTGAGTAATAAAACTTGTTTTGCCTTTAGCAATGATAGGTACGTTTGTCAGCTCCTTAACTATTTACAAAAAAGTAACAAGCAAAGAATGATTAGCAATAAAAGAAGAACAAATTAATTTAACGACAATGAATATTAATATTAATATGGGAGAGCAATCATCCAAAAACATGAATTCTTTTGAAAATCATGAATCAAATGAACGAAATAGTCTTCAAATTTATCAACAACATTCATCTATGATGTCAGAAACAAAGAGAAAACAAAGTAGCTTAAGTTATGATGCCAGAATTCTTTTACCTAAAAGTCCCTATAACACAAAGAAAACTTTATTTTTAATTATTTTCTTTTCCATTATCTCCTTGATATTGGCAACTATAGGTAGTGTGTTCATTTCTTTTGCTATAGTTCAAATTTCTATACCTTTTTACGTTATAGGTGGAGTTATTTGATTTTTTACTTTTATTTTCTTTATAGTAACTTTTGTAGCAATTGTAAGGTTCATAGCAGAGTACTTCGCTAATAAGTTAAATGCACTTACATGAAAAGTGGATCTTTCTGGACTAGAGATTAAACCTAAGGAAGAATAGTTATTGATTAAATTTGTTATTAAAAACCAAAAATTTAGTTTTTAAATAACTTTCTGTCTCTAAAAAAACGTTATCTTGTAAACTCAAAGATAGTTTCAGATTAGCTTTGCTTGCAAAAGTTTAATCTGTAAAAAGTTTTAATTCTATGGAGTCTGCACTAAACCAAGAGTTTCAAATTGATTTTTGTGTAAAAAACAAAAAACTGTTAAAAATTTTAGCTAACGTTCTTATTGCTTCTTGGTTATCGTTTGTTGTTTTTTTAATCCTAGGTTGTATAGCTATTGATCTTTTTCGATTTGATCTTTATTCCCAATTTTTCTTTAATCACTTAAGTACTTTATCTGCTTTAGCATGAACGTTTTTTGTATTAGCAATACTTTTTGGAGCAGCTACATTAGCCATTAATGGGTTTTTCTATAAAGAAATAAGAAAAAAATCAGCTTTTAAAGAAGATTCTAAATAGTTTCTTTTAGGCATTTTCTTTCCCCAACTTGCTTTTTCCAATGCGCTTTTCCTTAAATTTAAGTTAGCCCATTTGGATTTAAAAAAAGAACTTTTATTTAAAAAAGTAAAGCATAAAATAAAACTAATTAGTTTATTTATTTTTGGATTTTGATCAATTTATGTTGTTTGCATGACTTTAATTTATGTGCCAACTATACTTAATTTAATTGATAGTTTCAATTATTCCGAATCCATCTATAAATGAGTTGACTATTTCTTTCGCCATTTAGAAAGTAGAGATTTTTATTTTAGTAACTTTGGTTTTATATCACTCTTTTTGTTGCTTTTTGTTATTCCAACAATCACTCTCACAACATTAGGTTGCTTTCTATTTAGTTATTTACGGTTCACAGATATAAACAAAATAAAGATACAGATTTACAGCTTGTTAACTGTTTTCATTTTTATTGATGTTTTTGGTTTAGTGGTGTCTGTTCTGTTTGGTTATCTTTTACCTCTTGCTTTTGACTCACTGCCTTTTTCAGTGAATTTAACAAGAGAGGTGTTTTTGTCTTTAGCTATGATAGTCATATTTGCTAACAGTGTCATTTTTACTTTAAGGCAAAAAAGAAATATTGATTAATTAGCGAAAAAGAACGATTAAAGCTTCAAGCAATTGCTAATTTAAATATGGAAAAAGAAACAAAGAAAGATTTATTAAGTGAAGTGAAAAATAGCTTTCATTCTTCTAACAAAAATATTAAAAGCGAAATTATTTTGAAGAACACTTCAGAAAATAATATTTATTTAAGAAGCGATGCGAGAAGGCTTTTACCTAGAATCCCTTATAACACAAAGAGTGCATTATTATTCACCCTTTTATTTGCATTTCTATCTGGCCTTTCTTATGGTCTTGCATCTTTATGTATTAACTTCTCGTTTACTTCGCCACAAATTTTCATTGTCTTAATTTATTAGGGGTTCTTTTCTTTATTGTTGGTCTTGTTTTGGGAATTATTACCTTTATATGATTAATAAGATTCATAGCAGAATACTTTGCTAATCGTTTTTATGCACTTAAATGAAAAACCGATGTTCCTAGTACGTAAAATAAATCGAAGCAATAATTTAAAAATTGTTATTAAAACAGAATTTTAGTTTTTATCTAGATTTCCGTTTCCAAAAAAATGTTTCCTTGTAAATTTAAAGAAGTACTTTAAATAGAAAAAAGTGAAACAGAATTTAAGTAGTAGAGACTTTATTAAAAAGTCAGAAAAAACAGTATCAACTACTGTAAATATAAATACAGAAGCTAGTAGTTATATTGCCAAAAATCTAGAAAACCAAAAGGCAGTTCTTTCAGCAAAGGAATTAGAAAAACAAGAGCTTTTACTAGTGCGCTTAAATGCAGTAAAAAGATTTCCGTGCTATCCATACAATACAAAAAAGGCATTTAATATTTCTGTTTTATTTGTTCTATTAGCTGGTCTATTTTTTACTGCGTCTTATTTCTCTATTTTTTATTCGATTTTTACTAGGTTTAAAGCTTTACTTATTTTCTTAGGAGTAGTTTTTATTCTTATAACTTTCATTATGCTAATTCATTGCTATATATGGTCAGTAAGGTTCAAATCAGAGTACCTTGCTAATCGTATTTATGCACTGAATTTTCAAAAAGGAGCCTAATATAAGTGTTCAACAAAACCCAACAAAAGAAAACGTTAGTGATGTTAGCATGATAGAAATAAATAATTTGACCGCTTTAGAAAGGGATGAAAAAGTAGAAACGGAAGACATTAATACCAATGTAATAGCAAATACTTATACAGTGCAAGCCAATGTTCCAACTGCATTTCAAACCAGTGAAACAAATAGTAATACTGTTACTAACGTCCCAAAATCTCCAGAGTTAATAAGTATTCAAAGATTAGAAAGATTAAAAAATAGGTATACTTTCCTATAATTTTTTTCACTTTTAATTGGCTTCATTTTAGCCATATTACACATTGGAGGCTTTACTTTTGCAGGTGGTATTAATTTATTGGCCTTTTTAAGTTTTATAGATGCAGTTGCTTTTATTTGTTTTATTGTTTTCCTGGTTTTAGCAATTTCTACATCGCGAAGAATTAGAGACTTAGAAATGATGCAACAAATGAAAGAACTTGTTGATAAAAAGCAAGAGACACCAGAGCAAAATAAACAGTAAGTTATTTATTTTTAAGGGTTATTTATTTCTCTTTTTAGGAAATCCATTTGAAATATTTTGCGAATTTAGTTTATTCAATTCAAAAGACTTTTAAAAGAAATTAAACTGTTACTGTCGTGGAGATTAAAACTTACAAAATTAAAGATATTTGTGAAGCTTCACATGGAAGAGAAATAAATACTAAATATCTCCGTGAAAATCAAGGAATTTATCCTGTTTATTCATCAGCAACTTCTAATGAAGGTGAAATGGGCAGAATCAAAACTTACGATTTTGATGGTGAATATGTTACCTGAACTACACGTTGAAGTTATGCCGGTAGCATTTACTATCGAAATGGTAAATTTAGTGCTTCCTCAAATTGTGGAATTTTAAAAGTTTTAAATAAGGAAATAAATCCTAAATTTTTAGCTTATGCTTTAAAAAAAGAAGCAAAAAAATTTGTTAATACAACTTCTGCTATTCCTATCCTTAGAACGCAAAAAGTTGTCGAAATTCCCATTGATTTCCCACCCTTGCAAATTCAAGAAAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATTGCGATTATTTACTTAACCCAAAAAATTGAAAAGAGGAAAATAAATATTACAAGCTGGGAGAAATAGCTCAAAAAGTTTTAGTAGGTGGTGAAAAGCCTGCTGATTTTAGTAAGGAAAAGAACGAAGTTTATAAATATCCAATTCTTTCTAACAACAGTAAAGCTGAGGAATTTTTGGTTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGCCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTACCTAAAGAAGAATTTGATATAAGGTTTTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGCTCTGCAACAGGCCAGTTAACAGTGGCACAATTTAAGGAGTATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCGCTGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTATTCCTGCTGAAATTGAACTGTGCAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTGCAAAACCAAAAAGCAGCATCAATCCTGTAAGCAAAGAACTTTTTCTCTATGTTCTTTTAGTGCATTTTCTAACTGAAAATATCCAAGTAATTAGTTGTATTTTTCAGAAAGCTTAACTAAAATTCCATTGATTCGGTAGTTTGTTTATTTAAGTATTTGGCCAACATGACCAAATAAATTTTTTATGCAAACTATCTTAACTGATCAAGCTAGCTTCAACATATTAACTAGCAAGAGAAATTTAATTAATAAATTAACTATTACAAACATAGTGCTTTGAGCACTGTTTGTCTTTTGTGCAATAGCCACAGCCTTTTTAGCTATCAGCCATTCTTTTCACAAGAATTTTTCTTTTTTTCTGGAGTCTGGAAATTCAAACAACGAAGGTGAAACAACAACGAAAGTTAATATTCACGTTCTTGTAGCTCAAATATTAACCCCCATTTTTACAGTTTTACTGATTCTTTGTTGAATGGGCAACATCGGGATTAACAGTTTATTGTTCGGTCAACTAAGTATTTGCCAACAATTTCAAAATGAGAAAAAATGATTATTGTGAGCCATCTTTATCCCACAACTCACACTAACTAACTCACTGTTAGTAAGAGAAAAACTGAAGAACATTTTGGATGCAAATAGCAATAGTTCTACTCAAAAGAACAGACTTACTTGATGGATGATTGGCTTTTTTGTTGTTTGATTTATCCAACTTTTTCTAGGTTTCTTAATTTATTTACCAGCTTACAGTGCTGAAGCTCGCTTAAATTTAATTTATCTTTTTTATCCTTTGCCTTAAATTCACCTTTACCAGTAGGGCACGCAGCCAATGGCTATTTTTTCTTAGGTTTGATTTTTGCATTTATGGATTTAACCATAATAGTACTCCTAGTTATTAGTGGCTTCATCTTGAAGCATTTAATTCAAACTAATAAAACTGAGCACAAAAAATACCAACTAATGTTTTTCTGAACTGCTTTAGCAATTGATGTAATTGGGCTAATTATGACCATTTCATTTATAGTTGTTAGCTTTTCCTTAGAAAAAGGAGATGACTACTTATTAAAAACACCTTTCTTTGGTAGTTCATTGATGGCATTAATTACTATTGCAACGCTTGTAACTACAATCCTTTTATTTATTAGATTTAATAAAAAAAGATTACTTAAAAATTAATAAATAGTTTCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTTGTGCGCGCCGCCCATTTACACACCTCATACGAACTCGTGGACTGAAAGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGAGACAACCAACGTTCCCAAAAAAACCTCACCAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGACCGGTGTCGAACCCCTCGATTCGACCAAGGCTGGCAAGGGGAAAAGTTCCACAGAATGAAAAACGGAGGAGACAAATGCTAAGAGTGATGCTCCCCTAGCTAATGGTCAGCAAGTGGGGGCTGGAAGTGCTTTTGGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAGACCGCTCTTGAAGCACACCGCCCAAATTAATTTAAGGCAAACCCAAGACAACGCGCAAACTGGTAAATTCTCCAAATACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACTCGGCTCGACTTCTACAAATGAAGATCTGCCGAATGTAATAACAAAGTTATACCACACCTCCACGCCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTTGTATTGGGTTGTCGGGGAGGACCAGGAATCGGGTCGCGCAACCTGATGAGCCCACACCGAGCTCAACTGGGGCACCGACAAGCAGAAGCAGTTTGTCGAAAACCAGTTGGGTTTTAAAGATGACTCCAATTCGGATTCCAAGAATTCGAATTTGAAGGCTCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGAGCACGGAAAACAGTGCTGCCACCAAAGACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGCTCAACGGTCCGATTAATGGCTTGTTTACCCTGCTCGACAGCTTTGCCTATGTGACCCCGGTGAGTGGCATGAAAGGGGGTAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGCGCGCTTGCCGTCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAATCAACTTCCGACGCTAATTCGAACACCAAAGTCAAGTGAACCAACACCGCCTTGCACTACCTCCCCGTGCCGTATTACTATAGTGCCAATTTCCCCGAAGTCGGTAACAGAAGAAGAGCGGAGCAGCGGAATGGGGTGATAACAATAAAAAGGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTTAACACAAGTCACTCTATCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAATAGCCAAAAAGTTTGAATATTTTGCAATTTGGGTTTACAACTCCATCTAACTATTAGTAGGAACTTTTACTTTCAAAAAAACTTTTTGGTTTATTTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTTAGTGTAAGCACAAAAACCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCACTACTGCAAGTTGTAGTTTTCTCAACCTCTTTCAGAAAGGAGTTATTGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGATGAAAAAGACCAAAAAGGGCACTTTTTCGGGATGATACATCATTGACAAAGATAACAAAAGTGTGGAATTCTCTGGCAATTTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACAGAATACGTAACGCGTAAAGAGTTTAATGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATAAGAATCCAGGGTGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGAAAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGTTTGAACAAGATGGATGTTCGTCTAGATAAACTAGAGTCTAAGTAGATATTATTCAAAATAAAGAATACCAGCAATTAGTCTTTAAAAGATAGTTGTTGGTATTTTTATTCATTTAATACTTTCTAAAAACTTTTTGCAATTTTGTTGGCACTTCTCAATAAGCCGGTCAATTTATTTAGTGGAAGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACTTAGTTGAAAGTTTTATTTACTTTCCAAACCTTACAAAGAAAGGAGGATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCGAAGAAAAGCTTAAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTACTGATAACGAAGTAAAAAGCGAGGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTGATTTTTTGGTCTAAAAAGACATAAATTTACCAACAGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGACTGTTTTGGACCAGGTGTTGGATTATGTTCCCTGGATTGGGAATGGGTACAGGTATGGGAATGACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAACGTCCAAGCGACGCTCGGGGGCAGTCAGACGACGACTACCAAGGGTTCGCCTTGAAGAACCCTCGACCCAGCCAACCTCCAGCTCTGAACCGGCGCGGGGTGAAGGAATGATAAGGCTTCAAACAAACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGGATGGGCCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCCGATGCGTTAAAGCAGGATAAAATCACCAAGGAAAACTCGGGCAATCTCAAAACGGAGGATGGTACTGCTACTAATTCGACGACGGCATTCGCGTCGAATTACACCAACCTCCCCCCCAACCTCCCCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAATAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTCAATAAGAGTGGTGAAAATAACTAAAAATTCCAAGCCACCGACCAAAAATGGTCGTATGCCGACTTAAAAACAGACCAAACCAAACTCAACCTCCCCGCTTACGGCGAGGTGAATGGGCTAATATTTCTAAAAATTCAGTTTATAAGCAAAACTTATTAGTTAATCAACAGTTTGAAAAAGTTTTATTCTGAACCTATTAAATGTTAATAAAAGTTAATAGAAATAATAAAAAAACTTATGTAAGTATCACGAACACTCTTTTTAAATTCTTAGAAAAATGCACTTTTTTCCACTTTTTATAGCGATTTCTTTTGCATTCTTTAAACATAAATAAAAAGTTTTTCTGTATAATCTTCAGGCTGTTGACAACTCTGTCGACGCCGAAAATGTTCTTTCAAAACTGGATGCAATCTGTCAATTTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGAAAGTAGTAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAGACTAGCTAATACCGCATAAGAACTTTGGTTCGCATGAATCAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTGGGGTAACGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTAAGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTTTAGCAGGTAATGGCTAGAGTTTGACTGTACCATTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATTAATCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGGGCGATCCCCTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTCTAGCGAGACTGCTAATGCAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTCGCCAGCTTGTAAAAGTGAGCAAATCTGTAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGTCGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCATTAGGAAGCGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTTCTAATGGAGTTTTTTACTTTTTCTTTTCATCTTTAATAAAGATAAATACTAAACAAAACATCAAAATCCATTTATTTATCGGTGGTAAATTAAACCCAAATCCCTGTTTGGTCTCACAACTAACATATTTGGTCAGATTGTATCCAGTTCTGAAAGAACATTTCCGCTTCTTTCAAAACTGAAAACGACAATCTTTCTAGTTCCAAATAAATACCAAAGGATCAATACAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAATAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGTAGGTGGTAAGAACCTCAGATCCGGAGATTTCCGAATGGAGCAATCCGGTAGTTGGAAACAGCTATCATTAATTGATGAATAAATAGTCATATTAAAGCAATACGTGGTGAAGTGAAACATCTCAGTAGCCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACTTATCATTAGATAGGGGTTGTAGGGCTTGCAATGTGGACTTGAAAACGATAGAAGAAGCTGTTGGAAAGCAGCGCGCAAAAGGGTGATAGCCCCGTATTTGAAATTGTTTTCATACCTAGCGAGATCCCTGAGTAGCTCGGAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAAAGAGCGTTTGTTTGTTATTATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATCAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTGAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTCGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATTTATATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAAAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTTTCCACCCCCATAATTGGATTTGGGGATAAATCATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACATTGAGTGATGATGTCGAGTGAATGAGTGATCAAGTAGCGAAGGTGGTATTAATCATGCTTTCAAGAAAAGCTTCTAGGGTTAATCTAGCTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATGTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCGCAAGCGAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGAAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAGGACATTATCATGTAGAGAATAGGTAGGAGCAATCGATGCAAGTTCGCTAGGACTTGTTGATGCGAAAGGTGGAATACTACCCTTGGTTGTGTGCTGTTCTAATTGGTAACTGTTATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGTACAAAGGTACCTTCAGTACGGTTGGAAATCGTATGTAGAGTGTAATGGTGTAAGGGTGCTTGACTGTGAGACATACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTCCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCGTAGGAAGATTGAAGAGTGTTGCTTCTAGTACGAGAGGACCGAAGCGAGGACACCTCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCGCAAGAAAGTAAGAGCCGTCAAAGACGATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGAAGCCTTTGGTAGGAAATACGCAGGATTGTCGTTTTTGTTTGGTGCCCATGTCGCTGTGGAAACACCTGGTTCCATTTCGAACCCAGCAGTTAAGCACAGTGGAGCCGAATGTAGCTGTTTCAGTGAGAATAGGAAAGCACCAAGCGTCAAGAAGATAAAAGAGACTTAGGCCTATGCCTAGGTCTTTTTTATTGTTTTGGTAAGCCAAAATAGTCCATTTTATAGCTGTTTTTACTTTGCGAAAAAAACAGAAAACTCCAAGCAATCAATTAAAGCTTTGAGATAATTAAAGGCTTTGGTACTAGTCAAAACCAAAGCCGATTTTTTCATGAACCAACAATTAAATACCACCAGGAAAAGTACAGCTGCTAGGGGCAGAATGGGACTTGTTGGTGGTATTTTACTCGTCATTGGTACCTGCATAGGGGCAGGTATTTTCTTTAAGAGTGAACGGGTGTTGCAAAACATGGGCGGAAACACCACTTTAGCCTTACTGGTGTGGTTAATGGCAGGGATTACCGTGATCTTAATGGGGTTGGCGCTGGTTGAAATTACTGCTAAAGCAGCCTTTGATGACTTGGCATTATTGTCATGAACACAAAAGTTTACCAATAACACCTTTTACAAGGCCTGTAAGCGCTTTTTAATCTGGATTTACTTGCCCACTACCTTCTTCTTTATGCCGTTGTATTTGGTGCAATCACTCCAAGATGGTTTGCGTGGTTTTGGGGTCGCCAACCACTTCAATACACCACATGATTGGGCCATTTGAATGGTCATAGTGCTGTTGATTAACCTGTGATTTTTCTTTACGAGTGGTCTTAGTGTTAAGTGAACCAGTGTGCAGAATGTCGTGCTATTATTGCTCAAAGTAATTCCCCTAATAGCAGTGGTTATTTTAGCGCTGTGATTGGGTGCAAGTGCTGAACAAATGGAACACCAACCAGTGGTTCCAGTCAAAGATTTTACTGCCATTTTACCCTTCTTTGGTTGGTTTAGTGCTATGGGCGCGATTTTCTTTGCTTTTGATGGTTTTTACGTGTCAGCAGCAGCGAAAACGCAGTTGAAAAAACCAAAAAAATTACCGCAAGTAATGTTGTTGGGCTTAGGGATTGTGGTACTGATTTACAGCTTAATTGCACTAAGTGTGAGTTTAACCACACCTAACGGTGCCTTTTCGGGACTTGGTGATTGGTTAAAGCACAAAAAGCTTGGTTGATTCTTTGGCGTGCTCAATCTCTTAATAGCGCTTGGAGTAGCTGGGATTATTAACGGTTTTGTAATGTGAACGGGAAAGTTAACCCAAAGCTTAATTAAATCAGGAGAGTTGTGGGTACCTGATAAGTGTAAGCTCTGTTTAAATAAGCCCAAGCCAGTAGTGGGTTTAATCCATGCAGGGATTTTGATGGTGTTAACTACCGTAGCACTTTCTAGTTTAGGTGGTTTGTTGTACTTACCTAAAGTTAATGCTTCCTATGACGGCAAGGGCTTTAAATCAATGGGGTGCTTACTGGAGTTTGCTGACTTAATTGCCACTTGGACTAGTGTCGGGATCTTTTGGTTCTTGGGCTTAGTTTTACTGGGTGGTTTATTGCAAATCAAGAAACCGAAGCGGTGGTATTTTAGAACAACGGGTTGGCTAGCTGTAGTAGTGATTGGTCTCACTACTTTAGTGGTCATGGTGCAACCGTTTGTAGATTTAGGAATAGCAGTGTTTAACCGTAGTTATGAACGGATTGTGGCTAACACTATTTTGATTGCTATTCTTGTGATAATTGTTTTAGTAATGTTCTTTCCAACCGAACCAATTAAGCTAAGACTTTGACGCAAACGAATCCAAGCCATGGAAGCGTGCGGTGAGGATTGTGACGCTTGCGTGGAGTATTAACTAAAACTATAAAAAATAAAACTGGATAACCAAGCATGTAATATGAACAAGTTAAAGCGGCATAGTTAATTTGCGCTTAATTTCTTTTGGGGAGTTACACCGGGTAGAGGGCGAGTTTTGCTGGTATGCTTAGCGCGTTTTTGTAAGATTTGGCTTTCTTTCTAGTGTTGCTGAAATGCAATTTAAGTATGGTGCGCTAATTTTTAGCGGCTTTTTAGGACTTTCAATAGTTCTAGCTAGTTGTGGGGCGAGGGGTAAATTTGATCAAGTTGATGATGGTAAGATTAAGCTAGCTTCATCTTTAACTTCTAGAAGTGCGTCAGCGGCTTTACAAAAAGTTGTTGAAAAGTACAACAAGGTTAAAGGTGTAAATGATTACCCCATTGAGATTACCCAGATTGCTGGTGGTTATGATGGCGGGCGAACTGATCTCCAAACCCGGGTTAGTGTAAAGGATAAAACCAGCTTTTACAACATGATTCTAAACTACCCTGATTTAGTGTCGGTGTTAGCCCGTAACGGGATGGAATTACCGTTTGATGGAGTCAATGTTGACAAGTTATCTCCCAACTTCTTAAAGTTCAATGAACGCATTAGTGGCGTAGCGAAAAAAGCTAACTATGCCATTCCCATTTCGATGTCAACCGATATCTTAATCCTGAATGCACCGGTGTTGCACTATATTTTGAACAGTGTTAAGAAGAATGATGGCAACACTAAAGTTCAAGTTAAAGCTCAGTCAAAAGATTCACAAACAAAAGTTAAAGGTACTATGGAAATTGGCACTGATGAAAGTACTAAAAACTTATGAAGTGATATTCAAAAGAAAGCTGGCGAAAATGGCAAAGCTACTACTGAAGGCACGAAGAAGGCAGCAGCAAAGTCTACTCATTTAACTCTGTTAACTAAGTCGGAACAAAGTACTCAAGGTAATAATGGAGCATCTGAAAGTGATAAAAAAATTGAAGAAACTTGGGGTACTTACAGTGAAGTTGATGGTGGTCTAAAGAATTACACCTTTAAAGCCGATGTGTTTGATACTTGACATGGGTTGATCGATTTTTCAACGCGCGTAGCAAAATCATTTAAAAACAAAGTTAGTGATATTTCCACTAAAAAGGGCACTGACATTCAGGGCGTTTTAGGTCTAGACAGTACACCAAATGCTTTGTTTACCTCAGTTTTTGCTGCAGGTGATAGTAACTTTGATAACTTCTTTTATAAGGTTAAAGATGGTCGCGCTGACTTTAGTAACTTTAACGAAAACGGTACCTCTTACAAAAATCTGGAAAAGGTTTTTAATGACTATAAAAAACTCACAGACAGCAACGGTTTGTTTGTCAATAAAGGGGGTTCTTACACCTCTAACTTCCAGAAGTTTCACCAGTTAGCCTACAGCATTTCCTCCTCTTCTGGTTATGCTTATGCGTTTGCTGGTGAAAATTCAAAACGTTTAAAGTTCAACGACGATACTTTCATTGAGTACCCTAGTTTTACCCAGGAAATTCATGCGCCTGGTCAAAGTAGTCAAAAAGAAGGTGGACAGCAACAAAGCAACAGTAAAGATAACGGCAATCTTTTAGGCACTTTTACTATTGAAGCAGCGAAAAGTAAAACAAAAACAGAAGTTAAAAAAACTGAAGACACACAAAATCAAGGCAAGAAGGCTGAAGGCACACCTAACCAAGGTAAAAAAGCCGAAGGGACCGAAAACCAAGGTAAGACTATTTTCCTTTATAAAACATCAATTCCTAATGATAAGCAAGATGGTGTTGACGCTGTATTAATTAAAGACAAGTAGTTAATTCAAAAACTAGAGCAAGCTAAAAATAATAACGGAGCCGAGAAAAAGGATAAGTCTGAAACTAAAACCAATGAAAACAAAGGCACAGTCATCGGTTACACCACTACGAGTTTCCTGAATGCTGACAAAAAGCATGTCTTTGAAATTAGCAAGCTTAATGATGAAAAATACGACCGCAAGATTATCGTTGGTGCTACTGAGGAACAATTAGATCAATCAAGTACGTTACAAAGTGCGGAAGCAATTATCCTGCCAGCACCTGGTAAATACAGTGCCGAAAACCAGCATAAGGTAATGATGACTCAAGGTCCTAATCTAATTGGTATCCATACTAATACCAAGGAAAATGAAGAAACGCAAAAGTTTGTTAACTGGTTCTTAAATACCAGTCTAACTTGAGATAACAACGAAAGCAAAACACCAGCGCAGTACTTTACCGAGTCTGCTTCTTACATCCTGCCTTTAAAAGAAACTTTTACCGGCTCTAACAACAAAGGTCAAAGCGGCAAGAATGACGGTAAGAATTCCAATAACACCTTTAAAGCCAAAGCACTAGAGCTCTTTAAGGAACAGAGCGAAAACAAAATAGTGGGTTACAGTGATCCAAGCGATTTCAGGGGTGGTAAATTCCGCGAATCAATTGGTTCTGCCTTTAACGCCACGGTCAATTCACACGTTGACTTTAATACTTTCGTTGCTAACTTCCGTGCTAACTTAGGTTCTGGTTACGATAAATAGTTAATCCCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTCGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGCACAGGTATGGGAATGATCACCGGGGTGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTTTCGAATGTCGGCGTCGGTCTCAAAGCGAATGTCCAAGCCACCCTCGGGGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGACCCACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTACTGGGAATAACACCTAAGGCAACGAAAACCACACCAAGTTCGCGAGTGCTACGGGCACGGGCCAGCAGCAGGGTGGTTCTTCAGGTACAACTAATTCCGCGGGGAATCCCGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGACCGGTCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCTTGTTGGGCAGCATCCCGGTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCTCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCTTTGGTAGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTCGGTAACCTCGTTGTCAGTGGCACCACGGTGAGCTTCCAGCTCGGCGGGTGGCTGGTCACCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGGCTCCAGTTAAGTGGCCTGGATGCCAGTGACAGCGACCAAAGGGAGTTAATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGTTGGGCCGCGTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTGCCACCACCACCGCAACAGGGGCTACCTTACCGGAACACCCGAATGCTTTGGCGTACCAAATTAGCTATACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCTCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTCGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTGTTTGGAGCCATGAGTGGTAACCTCGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGGCAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACCATTAATAGGAATTTTTCTTATTAATAGGAACTTTTCTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAACGAGGCTTCACCGCTTTTTAAAACAAGTTCAATTGCAACTTGTTCCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTATTGGCGCTTCTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCAGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGGTAAAAAAGACCAAAAAGGGCACTTTTTCGGGATGATACATCATTGACAAAGATAACAAAAGTGTGGAATTCTCTGGCAGTTTTAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAATTTAACGAATATAAAGTTTCAAACGACCAACGGCTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAGGTTGAGCAAATTAAAGCTCAAGGAAAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCACTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAGTTTTTAGGCTAATAACAAAAGTGCCAGCAATTAATTTTATAAAGCTTATTTGCTGGTATTTTTATTCACAACCAATAAACAAATAAAAAGCCATCCACCGCGAGGTGAACGGTAGACGGCTAAAAATTTTTAATGATTTTGAAAACTAACGAAAAGTGTTAGTTTGAGTTGTAAAGCAGTTATGAATGTTTGTTCCTTTAAAAATTTGTTTGTTGTTTAGCTAAATATGTGCTGAAAGCCATTGCGTTAACATAAATAAATTAAGACATAAAAAATAAACACATTAATCAAATAAGTGTGGGAACAGAAAATCAGGCTTTCAACACAAAATTTTCGTTTTTCTGCTCCCACTAAATAAATTGAGTTGGTTTTTAAAAACCGCCAAAAAATTTTTAATTTAGTTTTTGCTACTTTCCCCTCCCCCTCACGGGTGAAAACCCCGGCGCTTGGGCCTTAGTGCGCGACAACAGCGCCAAGGGCATCACTGCCGGTAGTGAAAGTCAACAAACCACGTATGATCCCACTCGAACCGAATCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTAGTAGGTTAAACCCACAGACACCAACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGAACAAGGCACCGAACAGGTGGAACTTGAATCTAATGATCTCGCCAACGTTCCCATTAAGCGGGGCGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGAAGGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAATCGTACCGTCATTGATCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGGTTGTGGGACTGAAAGGCCAGAGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGGCGCCACCCCGAGTGGTTTGACCAGGGCCAAGCGGTCGCGGATAATACCCAAACCGGGTTTGATACAGATGACACCGATAATAAAAAACAAGGCTTTCAAAAGGAAGCTGACTCCGACAAGCCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAATTAATAAATAGGTCTATGGATTAATTACCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGGGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAGGCTTAATGATGGGACGAGTTCGGATTCCAATACCACCACTGATGGGGCGCTTACGTTTGACACGAATGAAAGTCAAAAAGCTTTAGGCTCCACGGACACGCAGGGTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAGTCTGGGTGAGACAACCAACGTTCCCAAAAAAACCTCACCAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTTCTAGTACCAGTTTGGGCAATGTTGGGGACACCACTGCCCTAACTCCACTTTTAAGAGGTGCTACTACTACTACCGTCCAGCTAAAACAAACAGATTCTAACAGTCAAGATCAACAAAAATTCCAAAAATATTTAAACACCGCCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCAGAACTTAGCCAAGGCGGTTGGCAAAGCCAAACAGCGCGGCACAGTTCCACCCGGGGACTACAGTCAGCCGGGGCCAATCGTGGCGCTAGTTTAGCGGCACGCACCACCACAACTGTGGGGGTGGGTAGAAGAGATTCCACTTCCACCACCTTGGAACTACCAAACGTCATCACCCAGCTTTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCGAAGACACATTTGAACTTCGGCACTGAAGTTCAAAAAAACTTCGTCGAAAACCAGTTGGGGTTTAAATCCGAAGACAACTCCAACACCTCCTTGACGAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACTTAATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGTACAACAACCTGGTGACGAATACTGCGGGGTTAAACGGTCCGATTAACGGCTTGTTTACCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATAACGAAGAAGTGCAAACGACCTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCGTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGTGGATAATGGCGATAAAAAAGCCATCCACCTAAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACTATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTACCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAGTAGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTACAACTCCATCTAACTATTAGTAGGGTTTTCACTTTTGAAAATTTTTTTGGATTAATTTTGGCACCCCGCCTTTTTTAAAGGCATTTAAGAGATATAAAAGAACAACTCATGTTTCTAGATACTTTCAAAAGATTTTTCGGGTAACTTAGATCACCTTTAATGTTGTTGTGGTTGGAGTTGATTTGGTTTAAATGGCAAAGGTTATGGTAAAGGTTTTTGGCACCTTTCTTTTACTTGTGCAATTCATTTATTAGAAGGGCAACAAATGGTCTTTTTGAACTAATCAGTTGTTGCCTAGCTTTATTTATTTAAAAAATGATGAGTAAAAATAAATTCTTTACCATTACTAAAGAACAGTACAGGAAGTTTACACGCAATAAAATTGAACTTCTTTTTAAGGTCCAATGTACCGAAAAGAAAAAGGGTAAACGTAGGTTCAAAATATTTCAGCAAGTCGAGGATAAACCAAAACCACCAGTTAAAATAATTGAAAAGGAATCTAAGGGACCGGAAGAACCAAAGGATCCTGGTATTAAAAATGGACCTAAGGGTCCCGGTGGACCAAAAGGACCTAAAAAGCCACCTGTTCCCGATGATCCTGATACACCAGATGTTCCTAAAGGCCCCAAAGAACCAGGCGGTTCAAAAGGTCCAGGTGGTCCCGATGATCCTAATGGAGGAAAGAAGAAAGTGCCTAAACCAGATAAGTATGTAACCCATCGCGAGCTTGATGAGAAACTTACTGAGTTTCATGACAAGCTTATGGTAGAACTTGATGGTAAGTTTGCTACTAAAGCTGATTTAAAACGGGTTGAAGATAAAGTTGATGTTTTGTTTGAATTGCAAAAAACGCAAGATGAACAAATCAAGGTTCAAGGAAAACAAATCTAACAGTTAACTGAAACTGTTCAAAAACAGGGCGAACAAATAAAGGAACTTCAAGTTCAGGTAAAAGCCCAAGGTGAAGAAATAAAAGAAATTAAAGTTGAACAAAAAGCTCAAGGCCAAACCTTGCAGTTAATTCTGAAAGCACTTGAAGGAATAAACAAGCGACTAGATAATTTGGAATCGAAATAATCCCATTCCAAAATAAAAATACCAACAGTCAATCCTTAAAAAGTTCACTGTTGGTATTTTTATTCACTCAAAAAATTCTTAAAATTTTTTTGTAATTTGTCGTCACTTCGAAATAACTGAATCAATTTATTTAGTGGAGGAGTAATTATGAGTAACTTTGAATTTTGATTAATCCTTCAGAAAGGAGGATTTGATTATGAAAGATGAAACAAATATTTGTTCTCAATCTTTAAAAAGACCTTTAAGCGAAGACCAATTGATAGAGTATTTGAGTACTTATGAAATAACTTTATTTGAGGTTTATGCTGAGGATATGCTAGATGGCAATTGCAAAATTTATATACATGCTAAAGGACGTCCAAAAGACCCAGATTGAAAAAGAAAGACTGATAAAAAATCTTTAGAAATTGAACGATATGTAAAGAAATTTATTAAGTTTTTAAAGAGAGAACAGAAAAAATAAAAACAAAATACCAACAGTTAATTCTTAAAAAACGCGCTGTTGGTATTTTTATTCAACGCAAATAAAGGAATAAAAAAGCCATCTACCCAGAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGCCTGCTCCCACTAAATAAATTGACAGGGTTTTATAAAAAAGCCAAAAGCTTTTAAAGTAACTATTTATTTGCAAATTAGTGCAAATATTCGTGTGCTATTTAAATTAACTATAGCACAATTAATAACAGTTGCATGATTGATCAAAGTAAGTTAATTGAAAGGTGAAAAACTACCTTTGAAACAGCACAAAATCCTACTGAATTATTAGCTTTTAAAAACAGCTTCCGCAATGCTGATTTAAAGCCTTTACTTAGTCAAATTAAGGAAACCACGGACATCGAAACTAAGCGCCATTTAGGCCAACTTTACAAGCAATTGGAAAGCACCTTGCAAACACTCCATGATACACAGCTACAAGTCTTTACCCAAGCCCAGTCTAGCAGTGTATTAACTCATGGTGATGTGATGTTGCTAGCAACCTCTTTTGCCCCTGGTTCTAGCAACATTATTTACCAGGTAATTGATGAGTTAGTGAACTATTTTAAGAAGTTCTTGTTTACGGTTAACTATGACAGTGAATTGACTACTATTGCAGACTGTTTTGACCTGCTAAACATTCCCAAAGACCATCCTAGTCGTAACCTTACTGACACCTTTTACTTAGACAAAAACCGCTTACTGAGAACACATTGTACTGCTGCTACTTTACGTGCTGTTAAGGAAACGAAAAAGAGTAATAACCCCGATATTCGGATTGCTTCCTTTGGTGCTGTTTTTCGTAAAGATGATGACGATGCCACCCACTCACACCAGTTCAACCAACTGGACTTCATGTGGATTAAAAAGGATTTTTCTTTAACTAACCTCAAGTGGTTTATGCAGAATATGATTAACCACATCTTTGGCGAAAATACTTCAGCGCGTTTTCGTCTGTCCCACTTCCCTTTTACCGAACCTTCCTTTGAAATTGACATCCGTTGCTGGTTGTGTCAGAATGGTTGTGGTGTATGTAAGAAAACACGTTGGATTGAGGTATTAGGGGCGGGTATCCTCCACCCTCAAGTGATGGCTAACATGGGCTTTAGTGACACGGATAATATAAGGGGTATAGCAGCTGGCATTGGGATTGAGCGGTTGGTAATGTTGAAACACGGCATTAGTGACATCCGTGACCTTTACGACAATAACTTCAAATTTTTAGCCCAGTTTACTGATTAGCGATGTTAATTTCTAAAAAAACTTTAGCTGTTTTAATTCCCGAAATTACCCACGTTTCCAACAATGAAATTTGTGAAAAGTTACAGCAAATCGGGATTGAAGTGGAAGCAATTCGCGCCTTTAAAAATCCTGATTACTTACAGTTGGGTATTCTCCGCGCTGTTACCCCCCACCCCCATGACAACCACTTGTATGTGTGTCAAGTTCAAATAGACAAAAACAAACAGCTCAATGTGGTGACTGGGGCAGTTAACATAGTTGATCCCAATAATCTCAATAAACACGTGATTGTCGCCAAAAAAGGGGCAGAACTGCTCAATGGTTTAATCATTAAAACGAAGAACATTAAGGGAATTATCTCGGACGGGATGTTGTGTAGTTATGTTGATATTAATCCGTTTAGTAAGCATTTAATTGCTGACGGTGATGACACGCATGCGATTGTGCTAGACAACATTAACCGCGATGAATTTGGTGATTATTTAAGTTTTTTAAACCTCGATGACGTGGTGTTTGAGGTTACTTTACCTACCAACCGTTCTGATTTACAGAGCTTAATCTTCCTCGCTAAGGAACTAGCAGCAGTGTTGAAACGTCCGATCTTCTTGGAACAGAAAACCACCATGACCCTGCGCGAGTTCTACCGCTTTCCGCTTAACTTAAGAAACCGTGCTCAGGCCAATTTCTTTGGTGGACTTTTTTTGCGTGATGTAGCAATTACCAGTTCCCCTTGAACTACTAAGGGTTTGTTAATTAACCAGGAGTTACGTCCAGTCAACTGCTTTGTGGACCAGGCTAATATGGTAACTGTTTACACTGGTCAACCAATCCACTGCCATGATGCTGACAAGATCCATGGAAGTGTGGAACTTAAACTAGCCACCCAATTGGAAACGATGTTGGCCTTAGACAACAAGGAGTATGAGATTAAACCGGGTGATCTAGTGGTAGCTGATGAACAGGGCACAATTGCCATAGTCGGTATTATTGGTAGTAAGCGCACTATGGTCGACAATACGACCAACAACATCTTTTTTGAAGTTGTTAACTATAACCATGAGCGAATTAAGCAAACCGCTCAGCGTTTAGGAGTTGCTAACTTTGCTAGTAGGTTAATGAGTAAACCAATTAGTTTACAAGCTACCGAAAACTGCTTGAACTATTTGCAAAATAACTTCCTCAATCCCGAAAGCATTGGCAAGATTAGTAAGTTTTCCAGCACCATTAAAGCCCCAGCTTTTAACCGCAAAATTTACCTCAACTTTAATCAGTTACGGGAGTTAATTGGAGTGACTAAAAAGCAGTTAAATGACTACATGATCCGTAACTACCTTACTAGTTTAGGGTTCAAAATGGAAAACCAAATTGCCCGCGCCCCCGCTTACCGGCAAGACATTACGGTTTGACAAGACATTAGTGAAGAGTTGTTAAAGATCTTGGACCTCAACAAGATTAAAGAGGATGAAATTTTAAGCACCACTAAACTAGAGAAACACGAAAAACTCAATGCTTACGATGCGTTGCAAAAGCTACGCACTAAACTGCAAACCTTGGGCTTTCACAATGTGATTACTTACCAGTTAATTTCACCAGAACGAGCGCGTAACTTTAACCTGTTTGGGTTGAGTAATTTGTGGGAAATTAAAAACCCGCTTTCCAACGAACGGAGTGTTTTACGGGTTGGTTTAATTGACTCCTTGTTGCGAGTAATCCAAAAGAATGCCGCATACAAAAATAAGTTAGGCAACATCTTTGAGTTTTCTTTTGTCAAAACTAAGGACAGTAACCAACTCCACATTGCTGCTCTTTGATTGGAAAAAATGTTTGGTTCTACCTACCAAAAGGATCAAGGCGTTAGTGTCGATATTCCCGCCATGAAGGGCTTAGCGCAGTTAATTATTTCCAACTTTGGTTTTAACTGCGACTTTGAACCAATTACTGAAGGTGAGTACTTCACTAAGAACGTCGGTTTAAAACTAGTGGTGTTTAACGAACAGATTGGTTATGTTGGTTTAATTAAGGATGAGCTGTTGGCGCCTTATGATTTAAAGGGTAGACCGGTGTATGGTTTGGAAATTAACCTAGACCGCTTGTTGAACTCCTTAAACCGGTTGGAGCGCAGTTATACCCCGATTAGCAAACTACAAGATGTCTTTAAGGATATTACCTTTAGCTTCCCCCGTGATGAAAGTCACTTTGAAACGTTTGTGAAAGCGATTAAAAAACTACAAACGATTTTTAAATGGGAATTGATCTCGGTGTTTGACACCGAAAAGGATGGTGTACCAATCACCAAATATACAGTGCGCTATTACTTAAAGAATTTCACTAATACACCATTAACGTTAGAACAAATTAAAGCAGTGGAAACGCAGTTAAAACAACAGTGTGAACTAGCTAAAATAGCACTCGATCTTTAGAAAACGTACTTTTTAAAAGATTTCGTTAGGTCTGTTCGGTTTTCGGTGTCTTCTTTTATTAAACTGATTCGGTTTCTATTTTTAAAGTAATAGAAATAAAGCTAGCCAAACAATGACAAACCAAAACCTACAAGACTTTCTGGACGGAGTTTTGTTAAAAGAGGAAAATTTAAACAAAATTACTGAAGTTTCCCTAGAAAAGGGATTACCGCACCTTTTAGATGAGGGTATCCATAACGAAGATAACTTTCAGATCTTTACGAGCCAAAAGATTGTGGCAGACATGATCAAAATGGTCGGTGTAAAGGAAATGAAAAATGTCCACACTACTGTTTTAGAACCAACTAGTGGTGATGGTGCTTTTACTTGTGCTATTTTGGATTTACGTTTAAAAAACATTAAAAAATCAGACAGCTTTTTAAAAGAAGCTTTAACCGGTCTATCCACCATTTATTCAGTTGAAATTGACGAAGAGTTGGTTTGGTTACAACGCAATAACCTTTACTCTATCTTTTTAGCCTTCTTAAAAAAGCACGGTATTTTTAACGAAGAGTTAAGTCTCTTAGTGAAAAAAATCTTGATGATTAATGTGAGTTGGGGCGAGTTAATCACAGAAACCAAAGATAACGAGAACTTCGTTTGTAACTACGATTATTTTTTAAGGAATAAAAAGACAAAGAAAAAACCCGCAAAGCATAAACATGAATTTCCAGAGGGTTTATTTTCCAATGGAGAAGGTAAGTGCAAAAGTTTGTTTGTTAATTGGTCATTTGCTGATGTCATTAATGAAATTGCTCATAATTCCAATTTTGACTTTGCCAAAATAGCAAAGATACCTGGTGAAATAATTCAATTTCAAATTAATTAGTATGAGTCAAAATGGCGATAAAGACCACGAATTGATTCAGGGAAAGGTCAAAAGCAAAACTGAAATAGAAGATTCCGTTATATTGGATCAAGACACTTTGCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGAGAAGCCGAGAAAAACGACTTGCTCAAGTTAATTGATAACGTCGGTAATGATGAAGTTTTTACTACTGTAGAAACTTGTCAAAGAATGCTTGACGATCTCTTTCCTCAAGACCATGAAGTTTGATCGAATCCGGACTTAAAGTGATTAAATCCTTGTGATAAAAATGGTGTTTTCTTTCGTGAAATTGCGTTAAGGCTAGACAAAGGCTTGGCCAAAGTTATACCGGATGAATATGAACGCAAGAAACACATCATGACCAAGATGCTTTTTAGTATTGACTTAACTAAATTTACTAGCTTGATGGTTCGTCGTACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAACTAGTGAAGATGAGGGCTGTGCAATTGCAAATGGCGCATGATTTAATAACGAATGCGGTAATGTTGTTAAACCTTACAAGGAACATTATTTCGACAAACAAGGAAAAAATAAAAAATGTAAGTTTTGTAGAACAGATGAGAAATTTAAATACCAACAATCTTCTTCAAATGAAAAATACGCCTATGACTTCATTCATTTAAATCCAAACGAATATGAAAGCTACTTTAAAACTAATTTTGGTGTTATGAAATTTGATGTAATTATTGGTAATCCACCTTATCAGTTAGCAAATAATCGTGGTGGTGATGCTGGTGATGGTAATGGAGCAAACCCCATTTTTCAAGAATTTATCTTAAGAGCACTTGAATTACAACCAAAATATTTAGCAATGATTGTTCCTGCAAGATGAATTAATTCCTCTGAAAAAGTTTTTCTAAAGTTCAGAGCAAAATTACAAAAATCAAATGGTTTTAAAGGAATTAATATTTTCTTTGACTCTAAACATTGCTTTCCAAACAGACAAATAAAAGGTGGTGTTTGTTACTTTAATTGACAGAATGGCTATGAGGGTCAAACACTCATAACCACTAAATCTTCAAAAAAAGGCAAACAAGAAGAAATTGAAACTTATAAGAGGGACTTTTTTGCACCTGTTTTAAACAATACTGAGCAAGTTATTTTCCGCAAAAAATTTAACACGACATTTATAAAAAGGTAGATTCTTTTCACGCTAATGAATTAATCCATGCTCTCAGAGAGAGAGAGAGAGAGCAACTTAGAAGATCAGCCTTCTAATGGAATAACACACACACACAGAGAGAGAGAGAGAGAGCAATTTAGAAACTAATTTCGAAAGTTTCTCTAAACGTGTATCTGGCAAGGATCTGTTTAATTTAGCAACTAATTTTTTTGATAAGTTTCCCTTAGTTGTAAAAGACAAAAAATTTCCTGGTGCAATTTCCATTTATAGCAGTTCGGGCGTAAAATACGCCGAACTGAAAGAGATCCCTAATATAAAGAATAAGGAACAAATTTCCAAATACAAGATCATTATGCCCAGAATAACTGGTAGCGGTGTGTTTGGTGATTACAGACCCAATATAAAAATTGTTGAACCTACTGGAGTTTGCACTCATAGTTATGTAGTTGCTTATTTAACGACAAATAAGTTTGAAGTTGAAAATGTCTTTTTATACATGAAAACTAAATTTTTTAGATTTTTTGTCGAGATTTTTAAAGCAACCATCAATATCAGTTCACATAACTTCAAATACGTTCCCATTCAAGATTTTTCCCGTCCTTGAAATGACAGGGAGCTTTATCAAAAATACAACTTAACTCAAGAAGAATGACAATACATCGAGAACAATATTAGTTCTTATGAAGATTAGCTTTAAAATTAAGGAACATGAAATTTAAGCTTAATTTTCACGAAAAAATCAACCAAAAAGATTGCTGACAATCTCTAATTGATCACAAGGAACGTTCCTACTCATTAGACTTCGTTAATAATACGGAAAAGGAACTACCACTTATCTATGGATACGAAGTAAAAGACTTTGAAAATCACGGGGTTAAAATTGGTTATACAACATGTAAACCGTCTGAGAAAATTCAATCAGCGATAGAAGAACGTATCCTTTCACAAGAAAAAGAATTTCGCTTTTTAGATGAAAATATAGAAAAGATAGAGGAAGTTAAAGTTATCTTTTGAGCTATTGCCATCAATGAAAAGGATGAATCATTCAAGGATTATTCACTCCATTCATTTATTAAGGAAAAAAACTTATTAAAAGAATCGCAAGCTGGCGGTGAATGGTTCATAGTTGATGAAAATAAGGATAAGTTTGAATATCTTAGCCAAATTTTTAGACAGTTTCGCGCACCTTTTCTTTTTAAAAATAAGGAAATTTATACACCTAGAAAAGAGCAAGCCGCATGTGTTGAAGTGTTAGAAAGATACTTTCAAGCCAATCCCGAAAACGGTAGGTTTTTAATGAACTGCAAAATGCGGTTCGGTAAATGTTTCACCTTGTACTCTTATGCACAAAAGAATAACATTAACAAGATTTTGATCCTAACGTTTGTTCCAGCTGTCGAAGAATCTTGAAAGGATGATTTAAATCACATTGAAAAGAATTACAAATTTTATACCGATGATGATTTGCAAAAATCTAATTTTGATTTAAAGAATCAAAACGAGCCTTATGTAGTCTTTTTATCACTGCAAAACGTCTTAGGTAAACAAAGAATAGATGGCGCCAAAACTGATTTTGATAAAGAGCGAATTAGTAAGCTACAAGAGATTGATTTTGATTTAATTGTTTTTGATGAGTACCACTACGGAGCCAACAAAAAACGAACCCAAATCAAAGTTGAAAAAGTCAATAAAAAGATTGATAATCCAGAACAGCAAGATAATCAAGATGTTGCTGAGGAAGAATTGGCAAGTACTTTTAAGTTAAAAGACATCAAAAGTAAATTTCAATTTTCATACAAACAATTAGTTTGTCTGTCAGGTACTCCTTTTTCCTCTTTAAGAAATAATGAGTTTTCCAGTAAAGATCAGGTGTTTACTTATTCTTACTTTGATGAACAGAAGGCCAAATCAGCAGAAAATCACCCACTCAAATTAGGGCAATACGGAATATTTCCGGAAATGAATATTTACTGTTTTGAATTGGCGGAAATTTTTACTGCACAAGAACAAGAAATATTCATAACTCCAGGTAAAGGTAAAAATAAGTTACCTGAAATTAGTTTTCGAAAGCTGTTTCAAACGGAAAACGTTTCTAAAGAATCAAGCAAACCAGTTTACAGATTTGTAAATGAAAACCTGGTTGAAAAGCTTATAGATTCTTTAATTGATAAACGTAAAGGTTTTAGCCACACACCTTTAAGTTGGGAAAACATTGACAAGCATAAGCATTCCTTATTAATTTTGCCAACTAGAGTTGCTTGTTTTGCGTTGGCCAATTTACTTAAAAATCACTGGTACTTTGAAAACAATGATTTTTAAATTATCAACATGTCAGAGAGCCAGTTTGGTAATGGCAAAAAAGCTTTAATTGAGCTTAATAAACACTTAGATGAAGCTAAAAAGACTAATAAAAATACTTTAACTATTACTGTAGCTAAATTAACTATTGGTATTACAGTTAAAGAGTGATCGACAGTATTTTTCTTGAAGGATTTAAAGGGTGCTGAATCTTATTTCCAAACGATTTTTAGAATCCAAACTCCATATATAAAGAACTGTAAAAATCTGAAAGAAATTTGTTATGTTTACGACTTCAACATGTATAGGTGTTTGGAAGTTACTAACGAGTATTCAAAACAAACACAAACAGACCCTAAATTTAGTGCTAGTTGATTCCAAAACTTAGACAAATTTTTACCTATCTATTTAGTTAGAGGCGATGAAATTCAAAAGACAGATCCAGAAATATTGCAGAAGTATGAGTACTTCATTATGGACAAGCGTGCATTCTCTACAAGATGAATGGATGAAAGTAACATTATCGACATAGATGTACTTTGCAATGTTGGGCAAGATGAAGATGCGCAAAAAATATTGAAAAAAATATTAGCTCATAAAAAATTTAAATCATCCAAAAAGAAGCGAGAGTTTGAAGACGTTCATTTGGAAAAAAGTCCAAAAAGTGAGGCATTTAGTGAAGGCGTTCGCTCTGGTAAGGATTACGCCGCTGAACAAGGTAATGTTTTAGAAGAACTTGAAAATTTCTGAAACTTTAACCAAGCATTAGAACAAAAAGCAAAAGCTGAATTTCAACAAAAAAACTTTGATGATAATGAATGAAACAATTACAAAAAAGGGTTTAATTTTGGAGTTAATAAGCATTTTGAAGATAAAGTTTCCATAAAGAAAATTGTTCAGAATAAAATCAAAGACTTTAAAAAAAGAAAAGGAAGAACAACTTCAACTTTTAAAAAATGAAAATGGTTATATTTCTGATGAGAATAACTTAAAAAAGCTAAGAGATTTAATTAAAGAATATGTACAAAACAGAAGCAACATACCTCCAGAATTAGACTGATTTTTTTACCGAGACCGTTTTATTCAGCTCTTTGAAAAGAGTTGTTACCAGCATTTCAAAAAGAAGACTTTAACTGAAGAACAAAAAGAGAAAAAGAAAAAAGTTAATGACAACTTTGATAGTTTAATGCATACATGGGCACGTGTTCTGCACTTTCTTTATGTATCTGTTTATAGAGAAAAAACTTTAAGAGATGTGTTGAATAAAACTGATCCCAAAGTTTTTTCAGTGGCAGTCGGAATTGAAATGGATGAGTTTTCCAAAATCCAAAAATTTTTTAAAGTGGAAAATATGAATAGACTCATCGAACAGTTTAGTTTTAGAGAAAGTGTTAAAACTGAAAACACAGAAAAATCACAGTATAGAAACAGTTTCAATTGATTCGGTTTTGGCCCCGATAGTTTAAAGAGCTAAATTTCACATTTTTAGTATGAACAAAAATAGTGGTAAAGACTACGAATCGATTCAAGGTAAGATTAAGAGTAAAGCTGAAATAGAAGATTCAGTTATATTGGAAAAAGAAACTTTGCAAGGCAAGGGTGAAGAAAAAACTAAATTAGATGAGTTGATTAGGTGAGAGGCCGAGAAAAATGACTTACTCAAGTTAATTGACAACGTCGGTAATGACGAAGTTTTCACTCCCGTAGAAACTTGTCAAAGAATGCTCGATGAGCTCTTTCCTGAAGATCATGAAGTTTGATCAAATCCCGATCTCAAGTGATTAAATCCGTGTGATAAAAACGGAGTTTTCTTTCGCGAAATTGCGCTAAGACTTGATAAAGGATTAACCAAAATTATTCCCGACGAATATGCCCGCAAGAAGCATATTATGACCAAGATGCTTTTTAGCATTGGCTTAACTAAATTTACTAGTTTGATGGTGCGTCGCACGCTTTACTATTGCATTAAAGCTAACAAGCGTAAAATCAGCGAAGATGAGGGTTGTGCAATTGCAAATGGCGCACGATTTAATAATGAATTTGGCAATGTTGTTACCCCTTATAAAGAACACTATTTTGGAAAAGAATCGAAAAGCAAAAACTGTCACTTTTGTGGGACAAATAAGAATTCTAAATATGTAAATTCTATGACTGAAGAAAAGTATGCCTATGACTTCATTCATTTAAATTCGGATGAATATGAAAACTACTTTAAAACTAATTTTGGTGTTATGAAATTTGATGTAATTATTGGAAATCCGCCTTACCAGCTATCTAATGGTAGCGGTTCTGATGGCAATGGAGCCAAAGCAATTTTTCAAGATTTTGTTTTAAAAGCAATTGACTTAGAACCAAAATATTTAGCGATGATTATCCCAGCAAAATGAATGATTTCTGCTGAAAATATTTTTTTAAATCTAAGAGATAAATTAAAGAAAAATAAAGGTATAAAAGAAATTAATATTTTTTTCGACTCTAAAGACTGTTTTCCAAAGAGAGAAATAAAAGGTGGCATTTGTTACTTTATCTGGCAGAATAACTATCAAGGTAAGACTCTTATAAATACCAAATTTTCAAAGAAAGGAAAAAGCACAGAGATCGATAGTTCTAAAAGAAACTTATTTGTTTCCGTTTTAAACAACACTGAACAAATCATTTTTCGCAAAAGGATTTGACAAGACATTTACACAAAGATTGATTCCATCTCTGCTAGTGAAATGAAGAACACACACACAGAGAGAGAGCAATTTAGAAGTTAGATTTCTTAATTTCTCTGATTACGTTTCAACGCAAAAATTCTTTAATCTAAGAAGTAATTTTTTCGATAAATTTCCTTCGATTTTGAAAGCAGAAAAATTTCCCGGTTTGATTGCAATTTATGGAAGTCATTCGGTTATTAAACACGTGGATATTAAAGATATTCCGCAAATAAAAAATAATGAAGAAATTGCTAAATACAAAATTATTGTTCCACACGTTTATAGCCGTGGCAATGGAACTTTTGGAAATTACAAACCAAAAGTAAAAATTATTAAACCTAATGAAATTTGCACCGAAACTTATTTAGTGGTATATCCCACCGAAAGTAAAGTTGAGATTGAAAACGTCGCTTCTTATATGAGAACTAAATTTTTCAGATTTTTAGTTGAAATTTTTAAGGATTCTATTAACACAAATTCTCAAAATTTCAAGTTTATTCCTCTACAAGATTTTTCACGTCCTTGGAACGATAGAGAACTTTATGAAAAATACGGTTTAACACTCGAAGAACAGCAATACATCGAGGCGAACATAAGTGCTTACGAGGACTAAATTGCCGCTAATTGAATTGATTAAAGTTTTGTTTTTAAGCGTTTTAAGTACTACTTTTAATACTTCGAAAAACGCAATCAAAACAAATACTCCCAAAACTAAATTTTAGATACAAAAATAACAACTAAACACATTAAAAAGTGGTTTGCTATACTCTTTTATTACTAGTGGTTTTACACATTTTTTCACTTAACAAAAAGTTTTAAAAAACACCAAAATTGCCACCAGCTAGCAAATCCAACACATAAAGAAAAACGTTCTTTTAAGGCTTATTAGAATAAGGGTAGATTATTCATTAGTTGTTGTATGCTAGATAAATTACTCCAGAAATTTCGAGACCAAAAGAAGCCCGTCTTCCACAAAGAAGAGGGCTACTGGGAAATTAGTGCCTTGCGGAAATGGGCAGCCATTTTAATAATTGCCTTTGGAGCTGGCATTATTTACATTGTGCCCTACTTTGCCTTCTTTCAGTTTAAGACAGCAGTAGCTAATGTAACGGGAGTGGAGCCCAACAGAATCTCCCTTCTCTTAACTGCTTACGGGATTGTTTCATTGCTGTTTTATATTCCTGGTGGTTGGTTAGCTGACCGGATTTCAGCTAAAGCGCTGTTTTCGGTATCAATGTTTGGTACTGGCATTATTACCTTCTGGTACTTCTTAGTTGGTTTGAAGGGCATAGTGTGGATTACACCCAACTAGTTATTATCCACGTCCTGTATGCCTTTAGTACGGTCTTAACCTTCTGGAGTGCCTTCATTAAAAGTATTAAGTTATTGGGTCCAGCGGATGAACAAGCGAGGTTGTATTCCACATCTGAAGGATCACGCTACTTAATCCAAGTAATTACTGGTTACATTAGTGCTGGTTTGGGTGCTATTGTCCTTATTAGTACTCCCTTTCTCATTTACAAAGCACCAACTGCTGCTGACCCTAAAAACTTAGCTTCTTCCTCAGGGATCTTCTTTGCCTTACTGTTTTATGCCATTTGTTATGTGTTAGCTGGTTTACTGTCACTGTTTGTTTTACCAGGAAAGTTCTGGGAAAAGCGCAAGATTTACTTGGAAGATGAACGAATTGAATACTTTAACCCTGTGGACCAGGCTTATCACATCTTTAAATCGGAAGCAGAATTAGAAGCCTTTAAGAAACATAACACTAAGGTTTTTTGAACGAAGGTATGAACTGATACCAAAGAAACTTTGAGTTCTACAAGCGTTTGGTTAATTGCCTTTCTGATCTTCTTTATTATGAATGTGTACCAAACGATTGCTAACTTTGGTACTAACATTTTGGAATCGCTAGGACTAGAGGCAGCACTCTCCCCAACTTTAAGTAATACCTATATCTATGGGATTCCGGTGTTAGGGGCAATCTTTTCTGGTTTGATTAACAAAAAGTTAACTAAATCAACCGCTAAAAGTCTGATTGTGCAAGCCTTGTTTGTCATTTTGGGTACCTTAGCCTTTTTAGTAATAACACTAGCTTCCCCAGCCAAACCAGAAACTGGTGCATTTAACCAAGCTACCTTGTGAATAGCATTTGTGGTGATTTGCTTCATCATGTTCTTTATTGGCGCGAACCGTTCCATTTTTTGGAGTACGATCACAGAACTGAAAGTCAATAAGGAAATAGTAGGACTGGCAGTTGGTTTTATTTCGATTATTGGTTTTTCCAAAGATGTGTGGTTAAGTCCCTTACTGACTGGTACTACTAACCAGTTCATTGTCAAAAACAGTCAGGGTACCTCGTTTTACAGCCAGCAAGCCTTGGTAGCCTGAGCCATTTTTGCCCTAATTAATGCTTGTTTAGCACTACTAGTGACTTACATGATAGTGCGCAAAGTGAAGTATGGCAAAGTGTGAGTTAACCCTAAATTTAAAAAAGTCTTTGCACTAGGAGAACAACAATATGGACACTAAATTAACACCACCCTGGTTGCGTCAAGAAAATAACCTGCTCAACCCCGAAGTTACCCAACGTTTGTTTATCAACCAGGACAACATTCCGAACTTACCCACTGAAGCCCCCCAAGTTTATTTAGCCCGTTTTTTGGAATGAGTTGAACCACTAGTCTCGAAAGTAAAATTTGTGAAAGCACAAGCAGCAGTTCAGGATTACCTCAATTCTAAAGCCTGTGCACAAATTGAAACCATTATTGCAGAACGGGCGCAAAACACCCATTCGAGTTGGTTAGCTAACTGGTGGGTGCAGTACGCTTATTTAACTTCGACTGGCCCGGTTAGTCCGGAAGTTAATGCACCCTATTATTTAGAGCTCCCGACTGTTGGTTGAAGTCAAGCCGAGTTAGCAGCAGCATTGTCAGCCCAGCTATGACACATATACCAGCAAGTCCAAAAACGGCAGTTAACGAGCTTTAGTGTCAAGGACAAGCTGTTTTCACTCGATACCTTGCAATCAATCTTTGCTAGTTGCCAGATTCACCGCGCTGATGGGGATGTGTACTTTGTTAATGATCAACCTGCTAACTTCATTGTGGTGATTAAGAACAATGTCTTCTATAAGTTAGTAATAGATAACAGTGGTAGCTTAGAACAACTGCAAGCGCAACTGCAGTTAAGCTTTGTGCAAATCTTAGACAATGAGCTAAGTCACCCACCGCACTGGAACTTACTCACTGCTACCACAACCAAAGCGGAGTCACAAAGTTTACTCGATCAGTTGTGAGCGCAAAACCCAGAAATGTTGTTGGACATCTATAACAGTGCCTTTATTGTGAACTTGGACAACGTCGAATTAACGACTCCCTTACAACTGTTGCGCAATTCGACATGAACCCCTAACTTTAACCGTTGGCACGCTAAGGGGATTCAGTTGGTCATTACTAAGAACGCTCAGTTAGTCATTCTAGCGGATCACACTAGTTTTGACGGTTCTAGTGTAGCTACCCTAGCCAACATCTTTGTCTCTAAACTCCAAAAGGTCAACACTGAGGGTGCCAGCGCTTTAACACCGACCATGCTTTCCTTTCCAACAGTAGACCAGGATAAGCAAAAGTATTTTAAGCAATTGAGCAAAAATTTTAAGGACTATGTGTACAATGCCGTTATGTTTGAACTGAAATGGGACTGGTTTACGAAGCCACTTATCAAAGCCAAAGGGATTAAGAACTCCGAAGCCTTTATCCATCTCTGTTACCAAATTGCCCAGTACCAAACGAACAAAAAGTTGCAGAACACCTATGTGGCCGTGGACATGCGTCAGTACTTCCGGGGACGCACCGAGTGTCTTCGTCCTTTAAGTAAACAATCAGTTGCCTTTGTCAAACGCTACTGCAAAGATCCGAAAGGTACCTTAAAGCAGTTCCGAAAGTACTACCCAGCGATTGAAAGTCTCCACTTTGAAAAAACCCGGTTAGCGCAAAAGGGTAGTGGGGTCAACCGTCACTTGCTGGGAGCATATTTGGCTTGGAATGAACACCAAGACACAATAGCTAAACCCGCTTTATTTGAAACAAAGGCTTGAAAAACGATTGCTGCCAACCCACTGTCCACATCGAGCATTGTGGACAAGTATTTGCGCAACTTTTCCTTCGACCCCGTAGAGCCAAACGGCATTGGTATTGCCTATGCCATTGATGACACTAATTTCCGTGCCATTCTCAGTGTGTATCAGCACAACTTACAGTACCTCAAAGATTGGATGAAACACTTTGAACAAACAGTGAAAACAATCCTTAAAACTCTTAAATAAAATTACTCCTTAATGGTTAATTGAGTTAAAACGGTTGGAGATTGTTAAAATATTGGCGCTATACAAAGTAGTCTTTACGACTCGCCCGAATGCCGTTGATTCTTGATCAGGGCTGTTTTGTTAGTGAAAGTAGATTTCATAGATTGGCACAGAACATAAAACAAGGTTCCAGGCGTGAGCGCGAACAAAAACCGTTAATTAACGATAAAATTGGCTTTAATGAGTTCATTTTAATTGACGAAAATGGCTCCAATTTGGGGACGGTTAGAAGGACCGATGCCCTCAAGATGGCTGAGGAAAAACAACTCGATTTAGTCTTAATTGGTTCAAACCCAGCCAAACCCATTGTTAAGCTCTTAGACTTTGGTCGTTACACTTATGATTTAAAACGCAAAAAACGCCAGTCCAAGAAAAACCAAACCATTATCCAAATAAAGGAAGTGGTAGTGAAACCGACAATCGCTAAACATGATCTGGAATTTAAAGCTAAGCAAACGACGGGATGAGCTGAAAAGGGTTATCACGTTAAGTTTGTAGTGCGCGCTTTTGGTAGAGTAAGTACTAGGATTGAGTTAATTGAAAAAGTCTTTAATGACTTTTATCTGCTAGTAGAACCAGCAGTGGAGGTCCAAAAACCACTAACTGCTTCTTCCAAAACAATGTATTCTGCTTTGTTAATTCCCCGTAAGAAATAAAAATTATGAAAGTAAAAAGTGCTGCAAAGAAGCGTTTTAAGCTCACTAAGTCTGGCCAAATTAAGCGCAAACACGCTTATACTTCCCACTTAGCACCGCACAAAACGACGAAGCAAAAGCGCCACTTGCGCAAGCAAGGAACTGTCAGTGCGAGTGACTTTAAACGCATTGGTAATTTAATTTAAGGAGTGAACTATGAGAATTAAAGGTGGAAAACAAACCCGCGTACGTCGCAAGAAGTGGTTAAAGCAAGCTAGTGGTAGCTTTGGTACCCGACATGCCTCTTATAAGGTGGCAAAGCAAACGGTGATCCAAGCCGCTAAGTATGCTTACCGCGATCGTCGTAACAAAAAGCGCGATTTCCGTTCGTTGTGGATCTTGCGCTTAAACGCCGCTTTGCGCGAACAGGGCATGACCTATTCGGTCTTTATCAATCTATTGAAGAAGCACAACATTGAGATTAACCGCAAAGTGTTGTCGGAACTAGCCATTAAAGAACCAAGTAAATTTAACCTAATTGTGCAAAAGGTTAAAAGTGAACAACCGAAAGCCGCTAAGCCAGCTGCTTTAGGAAACTAGCGACATTCTTAAAGGTAGTTTTACAGACCGCACTGTACTGGGCGAACTTGTGAAACTGCCTTTTTGTTTTTAGTAAGTGTAACGCTTCCACAATGCCATGGGCACTACCCTTGGGAAACTGTACACCGTACTGGTGGCTTAACTGTTCCATTTGTGCAATAAAAGCAGCACGTGCTAAGATCGAAGCAGCGGCAATTACCTTAGAATAGCTCTCACCCCTAATTAAGAAGTGGTTGGGAATAACTAATTCCTTTAACTCATTTTGCAGGCTGGTGAGTTTTTGCGTGTATTGGGTGAACAGTTTGACATTAGCAAACTCATCAATGCTAATGTCACATTGTTGTGCTGTTAGTTTGTTTTGTTGCAACAACTGGTGGTACACCTTGCAGTGCAAAAAGGTCAACATTACGTTGACATTTTTAAAGCTTTGCACTAGTTGGTTGTAAGTTTTAGCATCAACGCTTAATGTAACTTGGTCATGAACCGCTGCCTTGATTTTAGGCACTAAAGCTTTAATTTGGTAATCGTTAAATTGCTTCGAATCCCCCACACCAATTTGGTGGAGTGTATTGATTTTGTCCTTATGAATTAAAACAGCACTTACAGCAATTCCGCCAAAGCTATCACCCTTGCCAGATTCATCACTGCCAATTAGGTAAAGTGCGGTTGTGTTTGCGTGTTGCAATGGCTTATAAATTAATAAAATTATACCAACTCTACTTTCTTAGAATAAACGTTACATGGCAGAGGCAAAACGTGATTATTATGAAGTCTTAGGGCTTAGTCGGGATGCTGATGATAACGACATAAAAAAGGCCTTTCGTAAACTAGCCAAAAAGTACCACCCCGACCGCAATAAGGCACCGGATGCGGCTCAAATTTTTGCCGAAATTAACGAGGCCAATGACGTGTTGTCTAACCCCAAAAAACGTGCTAACTATGACAAATACGGCCATGATGGGGTGGACAATGAAGGTGGTTTTGCCTTTCAAGCTGATGTGTTTGATTCTTTTTTTGAGGAGATTGAAAAATCGGGTGCCTTTGATAATTTAAGTGAGTCTAACACCAAAAAGAAAGAGAAGACTAAAACTAAGAAGAAGGGTTGGTTTTGGGGCAAATCCAAACAGGAAGAATCTACCAGCGATACTACTGAATATGCCGATGTTGACGCTGGTTTAGAAGATTATCCGCCTCAATCTGATTATCCAGATGATATTCCTGATGTGGATGCCAGGATCGAAGAAGTGGATCAATCTGCCTATGCCGATGACATTCCCGATGTGGATGCGGGAATGGATTGGGAACAAAACGCAGAAGTAGCAAATTCTGCTAGTGAAATCATCCCCGATGTCGATGCTGGTTTAGCGGATGAGTTTAATACTAGTAGTGCAGCACCCCAAGCTAGTGACTGGGAAGCAATGATTGGTAATCCTGAGTACGGTTACTTTGATGCTGCTGGTGAGTGAAACTGAAAGGGCTTCTTTGATGAAGCGGGTCAATGGGTTTGACTAGAAGAGACTGAACCTAGTAGTGTCAGTAATGACGAAACTACGACAGATAGTGATGCAGTGACTGCTGCAACTACAGTAGAGGAAACGGATCAAGACAGTTGAACAGCAAACTCAGCACCAGAGTCAGTTGATGTAGAAACTCCAGTAGAATTGCAACCGGAAACTGAACCAGAACCAATTATTACCTTAAGTTCAGAGCCAGTGGAAGCACCAGCTTCTGTTGTGATTGAACCTACCCCTGAAACTGAGGAAACAACTAGTGCTGTAGAAATGGATGCTTCAGTTAAAGCAGATGTTTCTGATGAAGCTGACGCAACTAATGAACCAACTGAACAGGATACCATTTCAGAGCCAGAACAAGAAACAGACGCGGCTGCATTAGAAGAAATTAACCACACCACTGCTGATTTGGAACCAGCAGAAGTTAGTGCTACTAATGATCTAGAACAAGATGTAGTAGAGAAAGTTAATTTTTCAGAACCAGAATCAACAGTAGATACTGCTGCAACCGATCCTGTTGTAGAACAAGCTACGGAAACCTCCACCAATGGTTTCAAATTCTTTAACTTTAGTTCGTTTGTTTTAAGTGACCAAAACCCCAATCCACAAACACCCACCCACCATGAAGAAGATGCTGCAGCCCCCGAGCCAACCGTTGATGAAACTTCTGGAGAATCCACAGCCCCCGAAGTTACCATAGCTGAGTCAACTGTAGAATTGGAAACCGCAGCGGAAATTAATAACCCCGCTACCTTTGTGGAAGAATACCTCCAACCAACTAAAACTACGGTTGTTGATAAATTAGATGAACCAACTGTAGCAAAACCTACTGTTAGCGATTCTGAAAATTCAGTTGCACCAGAACCGGAATTTGTTGCTGGACCGGAACAAACCTTTAGCTGAAAACCAGCAATATCAGAAACCGAAGAAATTCCGTTAACTGCGGTAGAACCTGCTTCAGAGACTCAAACACTAATAGCAGAAGATGTAACTTCGCCAGTAACACCAACAGCCACAGCTATTCCCGCACCTTCAATTAACGCTGTACCAACTGCACCAGTAGCGGAAACCTTTGAGGCGGCGGTTGACTTCTTAAAGGAAGCTGCTAAGATTGAGGCACAACTACCACTAGTCCCAACAGTCCCAGAACAGATTGATGGAACTGATCCTAGTTTACTAACCCAGTGAGATGAGTACCTTGAAAAGACCCGCAAGCTGTTCCACAAGCTCTTTTTAACTGAGCAACTGCCTTTTATTGTTAAGACTGATCAATTTGAGATAGTTGACCCCAATTTAGACGAGCACAACGTTAACTTAATTTACACCGAACACGTACCCCAAATTTGTTTCTTGAACGAACAGCTCAAGGAAATTAGGTACACCCGTAAGTTAGTCGATCCACAAACTCAGGTAACCACCACTGAATCAATTACCCTAGAAGTTCAGCTATCACATAAATCCCAAACGGAAGCAATTGCCATCTTTAAGGGCTTTGGTCATGACTATGGTAGTGGCTGTGGTGACCTTAAGGTAGTACTTAAAGTGATCCCTAGTGTCTTTTTCCAACTGCAAGCTGATGGCTTACACACCGCTGCCCTAGTCGACCCTCTAGTGGCTTACAACGGTGGTTTAATTGATTTCTTTGGTCCAGTTAACAGCTTTAAAGTTGATATTGAAGGTGGGATTGCTAACAACGATTTAATTGAGTTTAACCAGTTAGGAGTACTAAGAACTAAGACCAAACGTGGTAGTTTATACGTCCACTTGTACTATAGTAGTGTAGCCAAAAAGGGCACTAAGACTAACTGCCAAGTGCAACAGTTCTTTGATCTAGTTCATGTGGAATACAAGCTGTTAAACTACAACTTAAAACAACTCCACAACTACCACTCGGCTCTCACTGCCCAGAAAAAAACCTTGGACCGCAAAAGCTATCAATGTTTAGCAGTGGAAAGCCATTAATTAGAATTACAATAAATTACTCATGAGCGAAAACTCACTCACAATTACCGAAATTCTCAGTTCCATCCGTACGCTTTTAGTCAAACACAATAAGGCTAAAGTAACCCAAATTGAAAAGGAACTTTTACAAGCAGTTGCTGAACTGGAAAAGAAGTTTAAGCAACAAGTACAAAACTTCAACGAGTTGCAACAAAAGATTCCTAACTTACAAAAAGTCAACGAGGAATTTCGCTTAAAAGTGGAAAAAATCCAGGAAGAAGCGCAGAAGAAAATTCAGGAAAAAGTGGCAGAACTGACCATTAAAAGTAAGGAAGAGTTGGAAAATGCAAAGAAGTATGTGATCGAAAAGTCGATTGATCAACCCTTAATTATTATTGACCAGTTCGAAATTGCACTTTCCTATGCTCAAAAGGATCCCCAAGTAAAAAACTATACCACTGGGTTTAACATGGTGTTGGATGCCTTTTCGCGCTGATTAGAAGGTTTTGGTGTTACTAAGATTGCCATTGAACCAGGGGCACAATTTGACGAAAAGGTGATGGCAGCACTGGAAGCTGTGCCTTCTGATCAACCGGCTAATACGGTGGTGAAGGTATCGAAATCGGGTTACAAACTCCACGACAAGGTCATTCGCTTTGCTTCCGTAGTGGTAAGCCAAGGTAATAAAACAGAATAATGAGCACAAATAAAAGAAGAACCATTCAGATTGAAATCACTGAAGAACACTTCAAGGATTTAGAAAAAGCCTTGGAAGCCTTGAAGGGTACCCAATTACCCTTCTCCACTACCGTAGAACAGTTTGTGGAATTAATTTTGTCCAACTATGTCGCTACTTCCAACAAGATTTCTAATTTAGCTGAAAGTGGCTTTGATGTGGCTTCTATTCAACAAGAATTAGAAAAAATTGGCAGCGCTGCAGGGGCGGATGATGCGCTCAAAAGCTTCTTGGATGAGTTGTTAAAAACATCGCAAAAAAGCTTTAGCAATACCAAAGATGGCAAAAAAAATGATGACGATAATAATTCGTCATCAAAATCATAAGAGGAAATATTAATAATTGGTGCCGAATAACAGAGTCGAACTGTTCTCTGATCCTTACCATGGATCTGTTTTGCCCCTAAACTAATTCGGCTTCAAGGATAATTCTAGTTTAAATTTGGACGGAGATGAAAAATAATTACAGCGAAGCGAATATCAAAATTCTCAAGGGGCTAGATGCTGTAAAAAAACGCCCGGGAATGTACATCGGCTCAACCGATAGTCGCGGTTTTCACCACTTGCTGTGGGAAATTTTAGACAACTGTGTCGATGAAGTTTTATCGGGCTTTGCTAATACTATTGCCGTTGTTTTACATGCCGAAAACCAGATAACTGTTAGTGACAACGGTCGTGGTATTCCCTTCGAAACACACTCCGATTCCAAGATTTCTACGATTGACACCGTCTTTACCTACCTCCATGCCGGTGGTAAGTTTGATAACGATTCTTATAAGATTGCTGGAGGGTTGCACGGGGTGGGGGCTTCGGTGGTGAATGCCTTGAGTGACCAGTTACAAGTAACGGTTAAACGTCAGGGCAAGGTGTACCGCTCGGTGTATGAAAATGGTGGTAAAATTAAGCAAAAAGCGCATTGCATTGGTAATGCCAAAATTGACGAACACGGTACCAGTGTGACTTTTCGTCCTGACCCAAAGGTCTTTAAAAAGATCCACTTTGACAGCGAACTAATTCGTGCGCGGTTAAAGGAATTAGCCTTCCTTTTCAAAAAGTTACAACTGACCTTCGTTGATGAAACCGGCAGTGGGGAAAAAGAGGTGTTCTTTACCGAAGCCGGTATTAGTCAGTACTTGGACGAACTTAACGCTGATTCCAAACAAATTGCGCAAAAGATCTTTGTTAGTGGCACTGAAGATGACATTGAACTGGAAGCCGTGTTTCAGTTTATTGATGGGGAGGATGAAAAACTACTTTCGTTTGCCAATTCGATTCGCACCAGCGAAGGGGGTAGTCATGAAGCGGGTTTTCGTCAATCTGTTGGTGATGTGATTAACAACTACTGCCGCAAGTACAACTTCCTAAAGGAGCGTGACAAGAACTTTGAGGCCAGTGAAATCCGTGAAGGCTTAAACGGCATTATTAAGGTCAATCTCCCCGAAAAGATTATTGCCTTTGAAGGGCAAACGAAGAGCAAACTGTTCTCCAAGGAAGTTAAAGCGGTAGTGCAAAAGCTAACCCAAAAGCACTTCTTCCAGTTCTTGGAACGTAACAGTGTTGATGCTAAATTAATTGTCGAAAAGCTGTTTTATAACCGCAAACTGCGCCAAGAACTCAAACAACAACGCCAAATTAAAAAGAACCTTTCTAATCCGAAGGCCGAGCGCATTTTGTTTGGTAAATTAGCACCGGCGCAGTCCAAAAAAGTGGCTGAACGCGAACTGTTTGTGGTGGAAGGTGACAGTGCTGGTGGTACGGCTAAAATGGGACGAGATCGCTTTCTACAAGCCATCTTACCGTTACGCGGTAAGGTGTTAAATGTGGAGAAAATTAACAACAAGAAGGAAGCGATTAACAACGAAGAGCTGTTAACACTCATCTTCTGTATCGGTACTGGTATTGGTAACAACTTCACCATTCGCGATCGTAAATACGACAAGATTATTATCATGACTGATGCTGACAATGACGGTGCGCACATCCAGATTTTACTGCTCACCTTCTTCTACCGTTACATGAAACCCCTAATTGAAAAAGGACACATTTACCTAGCACTTCCACCACTTTATAAGTTTGAAGGTCGTGATAAAAAAGCCCGTTATCTCTGAACCGAACAGGAATTGGAACAGTACCGTGCTAAGCATAGCCACTTTAATGTGCAACGGTACAAGGGTTTAGGGGAAATGAATGCTGATCAGCTCTGGGAAACCACGATGAACCCGATGACGCGTAAACTAATTCAGGTCAAATTGGATAACTTTATCCAAGCTGAAAAGCAAATTAATGTTTTTATGGGTGATAAAACCGAATTGCGTAAAAGCTGGATTGAAGCTAACATTAACTTTAGTAGTGAGAACTAATGGAAAAGAATAAACAAGCGTTATTACTCCAGGCCATCGAAGATGTCTTTGCCTTTAGCTTTAGTAAGTATGCTAAGTACATTATCCAAGACCGGGCTTTACCTGATCTACGTGATGGGCTTAAACCAGTTCAACGCCGCATCCTCTATGGCATGTACCAAATGGGCTTAAAACCCACTTCACCCTACAAAAAGTCAGCTCGGGCAGTCGGAGAAATTATGGGTAAGTACCACCCCCATGGCGATGCATCGATCTATGATGCGATTGTAAGGATGTCACAAGCCTGAAAGAACAACCTCACTACTATCTCCATTCACGGGAATAATGGTTCCATAGATGGTGATAACGCTGCCGCGATGCGCTATACGGAAGCGCGTTTAAGTCCGTACGGGTTTGAACTACTCAAAGACATTGAAAAGCAGTTAGTACCGTTTGTGAATAACTTTGATGACAGTGAGGTCGAACCGAGCGTGTTACCAACCCTGTTACCTAACCTCTTTATTAACGGTACCAGTGGAATAGCGGCGGGTTATGCCACTAATATTGCACCCCATAATGTCGGTGAACTGCTTGATGGTTTGAGTTACCGGATTGAAAACCCTGATTGTGATTTAAAGGCCATCTTAAAGATTGTTAAGGGACCGGATTTTCCAACTGGTGGATTAGTGTACTTTGAGCAAGAGTTGGCGAACATCTACCAAACTGGGAAGGGTAAGTTTGTGATTCAGGCCAAATATGAAACGAACACTGCGTTTGGCCAAAACCAGATTGTAATCACCGAAATCCCATACGAAACGGTGAAGGCCAATATTGTTAAACAAATTGAAGAGTTAATTAGTGACAATAAACTCAGTGCCTTGGAAAGTGTGATTGACAGCTCTGATCGTAGTGGTATTCGGATCATTATTAACCACAAGGACTTTTTGAGTGCTGACAAGATTATGGCGTTCTTGTTCAAACACACCCAACTCCAAGTAAACTTTAACCTCAACAATACGGTAATTGCCAACCGTTGTCCGGTACGGGTGGGGTTGTTAGCTTACTTTGATCAGTTCCTAGCCTTTGCCCACGAATTGATCATTAACAGCGCCAAGTATGATTTAGCATTGGCCAATAAGCGCTTGGAAATCATTAAGGGCTTAATTAAGGCGGTTTCGATGATTGATGAAATTATTCGTTTAATTCGCCGTGCTACTGACAAACAGGATGCGAAAACCAAGCTCATTGACAAGTATGCTTTTACCCTAAATCAAGCCGAAGCGATTGTGTCGTTGCGTTTGTACCAGTTAACTAACACCGACATTAAGGTCCTGTTTGCCGAACAAAAAGAGTTAGAGCAAACGATCCAAACAGCCGAATGCTTAATTGCTAAACCGCAAGCACGAAACCAGTTGTTACAAGCACAGTTTTTCCAATACAAAAAGCAGTTTAACCAACCACGACGCGCACAAATAGTTGGTTTAATTGAAAAGCAAAAGGTACAAGACAGTGACTTCATCGAACACAAGGAAGTGGGCTTACTCATTAGTCACGATGGCATCTACTTTAAGTTTGAACCAGAACAGTTAGCGAAGCACCTAGTGGAATTTAAAAGTGAACAAGACCAACTGATTTTTGGAGGCGTAGTACAAAACTCCGATTACTTTTTTATGGTGACTTCACTCGGTAACATTATTACCGTTCCCATTTACAAAACGTTAAGTAACACCAAAACCAAGATGAACGAGTTGTTGGCCAAAAAACCGATCCTAATGGAAGATGAAAAATTGGTACTAGCAGGGATTGTAAACCCAGACAAAATGGAACAACAGCTCTTGGTGTTAACGTCACAGTGCGGTATGGTCAAACGGGTCGAGTTGAGCAAAGTCATTAACACGAAGCAAATTAAGTCAAGCTGCTGCATGGCACTCCGGGAACGCGACAAACTGGTCAACGCCTTTGTTCAAACTAAAAGGGAACCTAAACTAGTTTGTCTGGTGTCATCTTCCAACAGTTTTGCTACCTTCTTAGCGGAAGAAATTCCAATTATTAGTAACAAGGGAATTGGGGTGAAGGGGATTAAACTCAAAGCTGAGGAAAAGGTACGGTTTGCAATGCCGTTACAGGATAATGATGCCTTAGTAGTGATTAACAGTGATGGTGGTGTCTATAACTTTGAAGTAGTGGAACTGGCAGTCGCTAGCCGCATGAGTGTTGGTAAAAAACTAATTCCCAAAACCAAAACCCCGGTAAGCTGTTTTGCTGCTAATAAGCACAGTGAGATTATTGGTCATCGTGGTAAAAATGGTAGTGATCTGTTTACCCTAAATGAGTTAAACCGTTTACCAAAGTCAACCGTTAGTCAAATGCGTTTGTTTAAGTGGAGCTAGTGGTTTTACCACCGTGAAGTTGCTTATCGTACTCCTCGATCTCTTCTTTGAGGGTCAACAATAACGCTTTCACCTTTTGGTAGTCCATCCGGGTGGGACCCACTATCGCTAGTTGGTGCTTACTTTCGTTGGTGGTATTAATAAGAGTAGAAGCTACCGAAACCTCTTCCCCTTCTAATCCTAACTTGTCACCAAACGTAATATTAGTAGTCTGGTTGTTTTGGTTCATAAAGGCCATTTGTTGTCAGACACTAGTATCTTCCAGTAAGTTTAAGATACGCGTCAATCTTTCCTGGTTGGCAAACTCTGGTTGTTGGGCTAGGTAATGGATGCCATAAACCTGCTTACGTGCTTGTTGGAATTCCTCAAAGTTAAACAGCTTAAACAGGATTTCGTCAATGACATACTGGTACTCGTGTACCTTGCTGCGAATAATTTCCTTTAAAACATCTAAGTGTTGGGCAATATCACAGAAACGTGTATCGATAATGCGATCATTAAAGATCCTGACACATACCATGATGTCTTCAAACTGTCTTTGGTTAGCATAGCTAATCGTTTTCTTAAAGACGTTACCACTAGCTGACACTAACAGTAGCAGTGCACAGGACTGATCGAGCATAATGAGATCAATCTTTTTTAAGACTTCATCACTACTAAAGTGGGTTAACACCACTGGTAGGTTTACCATTTCGTTGATAAACTTTACCCCCAGTTCAATAATTTCATCAATTGTTTTGTGTTTTTGCAAGATAATCGCCCTTAAGCGGGTTTTAAGGTTACTCTTATCATCATTTTTAGTGAGTAACTTGACATAGTACTGATAGCCCAACTGGGAGGGGATTCGTCCTGAAGAGATGTGGTTCTTTTTGAGATAACCCTCTTTTTCCAAAACGGCCATTTCGTTTCGGAGTGTACCGCCAGAGAGGTTTTTAAAATATTTTTTAGTTAATAATTTACTACCAACAGGTACTGGATAGGCAATATATTCGTTGATGATGGCCTTGAGAATTTGGGCTTGTCGGGTAGTTAAATTTTTCATTAGGTTATCCTAATTTTATTTTGTTAGCCGCTTGATCATCGTGAACAATACGCTCGCTTTTAAGTTAAAGAACGCACCCCATAAGCCAGGCTGCTATTTGTGAAAGGATGACGCTGGTCAAGTTTTGTATGTCGGGAAGGCTAAGGACATCTTTAAGCGGGTACACCATTACTTTAATCCAAACCGTTCCTTTAAAACACGGGCACTAGTGGAGCGAATTGCGGATGTCGAATACGTCATTTTGAAAAATGAAAATGATGCTTTGAACTTAGAGGCCAAACTAATTAAACAGTACAAACCGCGCTTTAATTTAGTTTTAAAGGAAAATAATGGTTATCTCTATTTCTTTATTACCGCCAGTGTTAAACCCACCTTGGAATTGGGCCGCCGGTATGAGTTTTCCAAGAATAAGTACTTCGGTCCGTTTGCATCGAGTAAGTTTCGACTGCGTGACATTTATGACCTGTTGTTAAAGCTTTTTCCATTACGCAAGTGCGCGCCCCATGAACGGGGTCACCCCTGCTTTTACTATCAGCTTAAAATGTGCATGGGTCAGTGTATGGGCGAGGATACACCGGAACGTTACCAGACCACCGTCAAGGGAATTGAACAGTTTTTTAACCACGGTCCCGAGCAGGTTTTAAACCACTTGCAACAGCAAGAAATCAAGGCTAGTGAACAACAAAACTTTGAAGCAGCACGCCACTTCTTGGACTTACAGAAAGCCGTTTTAGAACTAGTGAACATGCAACAAACGGCCTTCATTAAAGCAAAGCAAAGCCATGACTTTATTGGTTATGTCTTTGAGAAAAATGTGTTAGCCATTACCGTCTTTGCTTACGTTGATAACCAACTAATAGGCAAAAACCAGCAGGTCTTTGAACTACCACAGGACGATGAAAAGGAAGTTGAAAGTGCTTTGGTTACCTTTATTTACCACTATTATTCCACCAATAAGATACCCAAAACCCTTACAGTTTCTTTAAGCGAAGAGAATTTGAGCTTACTGGCCAACAGTCTTAAAATTAATGTTACGCAACCAAAAAACGGCGAGCAAAAATCCATTTTGCAAACCGTGATTGACAACGCCCGTTATGCCTTAAACACCAAGTGAACTGGGTTTATCAATAACCTTAACCGTGCCGAAGTGCACCAGCAATTAGCGCAGCTTTTACAAGTTCCTTCGATTCAAAGTCTGGAAATTATTGACATCTCCTTTTATGACAAAGACCACGTGGTGGGGGCGATGTTACGGTACGAAAATGGCAAACGGATGAAAGCGTTATCACGCCGTTACAACATTAACATTGACCATAAGGGTGACACTAACTATATGGCTGATGTGGTGTACCGCCGGATTATTTCGTCCATCCAAACCCATAAACAGCTACCTTTAAGTGATCTTTTAATTGTCGATGGTGGGATTGCCCAAATTAATACGGTAACCAAGGTGTTTGCCTCTTTCCCTAACGTTACCCAACCGATCATTATTGGCTTAGCGAAAAACACCCGTCACCAAACTGACCACATTGTGTTAACGGATAACACTACCATTAACATTGACAAAAATACGCCTTTGTTTGCTTATCTTACCACCATTCAAGAAGAGGTCGATAGCTTTGCCAAACACAATGCCTTTAAGCGAGTTAGTCGCGCTCGCTTCCAAAATCCACTCTTACAAATTGAAGGGGTTGGGCGAAAAACTGTCCAAATATTACTTGATAATTTTCAAACAATGCAAAACATTGAAGCTGCTTCTTTGAGCGAATTATCACAGTTTATTCCTGCTAATTTAGCACAAAAAATTAAAACTTTTCTAAAGGAAAATGACTAAAGTATTAGTGTTATCAGACACCCATGGCTATAACGATAGGTGACTAGCCGTTATGAAGTTGCACAACCCTGATGTAGTAATTCATGCTGGTGATCATTTAACTACTAAAAAGTTTATGGATCAAAATGCCACTTTTTGGGTTGCTGGTAATCATGATGTTGTTGGCGAAGAGATTCAAATGTTTGAGTTAGAGGGAATCCAGTTTGTTTTAATGCACGGCCATCAAGCGCCCCGTCATGATCTCAAGCAGTGGTATAAAATGCTGGTTGATCAAGCTAAAAGCTATCTTTGTGATGTTTTAATTGTGGGTCACAGTCACATAGAGCACTATGAAACTATTGATGGGATCCAGGTTATTAACCCCGGTTCTCTAGAAATTCCCCGTAATCCGAGAAAGCTACCTACCTACTGTAATTTAAACTTAAGTCAGGGCAGGATAAGTGATTTAACGTTCCATTTTCCTCGGGATTAAGTCAAATAAATAAAAAAAGCCATCTACCTAACAAGCAGAAGGCTAAAAAATACTTTTTTTAGCGATATTGAAATTAACAGAAAGATCTGTTAGCAAATGTAAGGAACAAGCTTATGCAAACTTTCAATATGTGACGTGTTGAAAAACCTTTAAGAGTATGCATCAATTGGGAGTAGGAAAGCAGGCTTTCAACACAAGTTTTCGTTTTCCTGCTCCCACTAAATAAATTGGGAAAGGTTTTCAAAGTCGCCAAAAAGATTAAATATTTTTGGAATTTTGAATTTTTAGTTGCTTCTAATTGTTAATAGAGATTTACTTTCAAAAAAACTTTTTGGTTTATTTTTGGTGTCTTTACTAACTTAAAGCAATTTATTTAGTGTAAAGACTCATAATGTTTAAATTAAAAATTAACAACTTCCAATTAGGTTTCAAACTCGTTCAGTGACCAGTGACGAACCACCTTCTTAAACATTTTTACGTTTTTCCAATCAATAATAAAGGAGGTCTTGCCATTAAACGCATAATTTCACTTGCTTTGTTTAAGAAAAGACTTAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGGATTTAATGGCAATTTCAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAACAATCAGCGTCTCATAAAGATTGAAACTACTTTGGCTATCCAAGGCGAACAAATCAACAAATTGACTCAAACTGTTGAAAAGCAAGGCGAACAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCAGGGCGAGCAAATTAGAGAACTTCAAGTGGAGCAAAAAGCACAAAGACAAGAGTTTAATGCCCGCATGGATCGTTTGGAAAATCTTTTGGTGGAAAGTATAGAATCTACCAATAATCGCTTCGACTCTATGGAAAGACGTTTAGACTCTATGGATAGTCGTCTTGATTCTATGGAAAATCGCTTGGTTTCAATGGAAAGCCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTCGTCTTGATTCTATGGAAAATCGCTTAGATTCAATGGAAGGTTGTCTTGATTTTGTTGAAGGACGCTTAGACTCTATGGAAACTCGTTTAGACTCTATGGAAACTCGCCTGGACAAAGTCGATCCGCCCAAATAGTTTTTAGTTAAAAAATAAGGGTATCAGCAGTTAATCTTAAAGATTAGTTGCTGGTATTTTTATTCATTTAATGCTTTCTAAAAACTTTTTGTCAATTTTGTTGGCACTTCTAAATTGCTAGTTCAATTTATTAAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGTAAAAAATCTGATCACCGTTGGTAGCATTGCTGCAAATAGTAGTTATGTAATTTGCAAACAGAAAGGAATTTTTCAATATGAGTAATACAAAAAAATTAAGAATAACTTCAATCGAAGAAACAATAGAAATAATAGTTAATTCAAAACTATTAGCTTTTGGTTTTTACCCAGTTGGTTTAGAAGATGGAACTTACGAACTTAATGTAAGGATTATTTTTAAAGAAAAAACTGCAGATTCTGAGAAGAGTAGCAGTGTACATCCTTTACAAAAGGAGTATTATGTAAGGGAATTTTTGAGATTTCTTGAGCGCCATAAAGATTCTCTGGCGCAACAACTATTAGTAGTAGAAGACAATTAAATAATTTCTCCCAAACTGGATTGACTCATCCTCACCGCCACCGCCTCCCTCGCGGCGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTGACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGAACGTATCGTAACACGAGCTTTTCCTCCCTCCCCCTCACGGGTGAAAACCCCGGGGCGTGGGCCTTAGTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGAACCTGCGCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAATAAGGTCCCCGTCGAGGTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGAGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATCCAGCAAGTCCAACTCAAGGCAGCCGACTTCGGCACCACCCCATCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGAAGGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCCTTTAATTGCTCTTGTTAATTTAGTTAAGTTTTTGTTTGCACAAAATTAAATTTTCTGTTTAATTAACTATCAATATTTTGCTGCTTTAAAATTTAAAGCAACTTTTAAACAGTAAGTTGTATCTTTTCACAAAGATTTAGTTCGATAAATTATTGGTAACAAGAGGGATGGAAACAACCGCAAAAAGTATTGATTGCAAAGAATTAGGCCTTTCTGTAACGACGAAGCAACAACTGCATAAATTAACACTTGCTACTATTTGACTTTGGGATTCTTTGTTTTTTGTTTTCTCTTACTTTTGTTTATAACGCTTACTTATACGATCTATTTTGAGCAACACATTTTGGGAAGTGATAAACCACTTCCTTTTTGGTGATACATTAAAAATCATGTGGTTCCCACCTTTGGCGTCTTAACTTTAGCTTGTTGGTGTGCGTTGATTGGGATTAACTTTTGGGTTTACAAACACACTTCACAAACAGAGCTGTTTCACAAATCAATTAGGTGACTTAAATGAGGTATTTTATTACCGCAAATTGCGCTAGCTAACTCCCTTTTCATTAGACAGAAACTAAGGCTAATGGAAGGCGAAGAACAGTTGAATAACAACAAAATAGAACAGAAACTAAAAGCTTTATTTATTAGTTTCTTTACTATTTGAGCAGTACAATTGCTTTTAGGTTTTTTCTTTTGGTTGCCGTTCTATGGTCTCTTTTTTTGATATATATACCAAAGCATCGATTGTAGAAGCTGTACGTTTTTCTTTTTTGAATACCTTTCAATTAAAGGAACTTGGTTTACCGATTAAAGAAATTGCTGTACCATTAGGCACATTAGTCTTTCTTTTTGTTGTTATTATTACCTTAATTCCGTTGTTAATTATTGGTAATTTAATTTGGACTAACTTGCGTTTAATAGAACGGGAAAATACACAGCAGTACCAACTCGTTTTTGGTTACAGTTTGATAGTTAGTGATATTGTTGGTTTTGCTATTGTTTTCTTCGGTGCCATTTTAGGTACAAATTTAAAAAGTGTTGAATTATTCATAGCATTGGGTTGAATGATGATGTTAGGATCATTAATTGCATTGGGCACTACTGCTAACTTAGTGAGCTCAATTTATCTCTACATAAAACTAGCATTGAAGAGATAGACACACTTCTCCCCCACGCTCACGCGCTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTTCTCGACTAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGCGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCTACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCCAACGTCCAAGCCACGCTTGGTGGCAGTCAGAAGACGACTACAGGCGGCTCCAAATGACCCACCCTCGACCAAGCCAACCTCCTGCTCTGAACCGGCGCGGGGTGAAGGAATGATAAGAACGGACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGCAGTGGCCAGCAGGGTGGTTCTTCAGGTACAAATACCTCAGCGGGGAATCCCGACTCGTTAAAGCAGGATAAAATCACCAAGGAAAGCTCGGGTAACCTCAAAACGGAGGACGGCGCTGCCAACTCTAACGAAAACAAAGACAACACTAATTATCAAAATTTACCCGCTTATGGGAAAGTTAGTGGGTGGATAACACCTGATTATTAATAGGCTTTTTACTTTCCAAAAGTTTTAAAGTTGTTTTGGCACTTTCAAACTTTAAAGACCATTTAAAAGGCATAAAAGGATAGTTATGTCTTTAAGAAATATTTTGCGATTCAGTTCGCTTTTGGTTTTATTTGGTTTTGAGTCTTTTCTAGTTAATGCTCAAATCTTTTTGCAAAGTTTGTTGTCACTTCTAAATAACCTACTCAATGTATTTAGTGGAGGAACAAAATGCAAATCTTTAATTTTTGAAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAAAAGAAAGGAACATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTTTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGGTACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGCTGACACTGAGACGACAAATAAGGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTGATTTTTTGGTCTAAAAAGACATAAATTTACCAACAGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTCCCCCACGCTTTCCCGCTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACGACCCAGGGACAATCCCAAAATGCATCCAGTAACGAACCCGCACCGACGTTTTCGAATGTCGGCGTTGGTCTCAAAGCCAATGTCAACGGCACCTTAAGTGGTTCGCGAACTACGCCTAATCAGCAAGGCACTCCCTGGTTAACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGCGCGGGGTGAAGGAATGATAAGAACGGACAAAGTGACGAAAACTACACCAATTTCGCGAGTGCTACGGGCAGTACGAACCAGCAGGGTTCAACAACGGGTGGATCAGCGGGCAACCCCGACTCGTTAAAGCAGGATAAGGCTGATAAAAGTGGTGATTCGGTAACGGTTGCAGAAGCCACATCGGGCGACAACTTGACGAATTACACCAACCTCCCCCCAACCTCACCCCCACATCCGACTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCCCAGCGGGTGCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTCAATAAGAGTGGGCAAGATGATAACAGTAAGTTTAATTCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCTTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCAGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTCGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGCTCGGCGGGTGGTTAGTTACGGTTCACGGACTTTATCAAACCCCGCGCGGGTTATTTGGGCCTCCAGTTAAGTGGCCTGGATGCCAGTGACAGCGACCAAAGGGAGTTAATTTGGGCCAAGCGGCCCTGAGCGGCATTTCGTGGCAGTTGGGTCAACCGGTTGGGACGGGTCGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGACACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTCCACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGCTCCGGTCAAACCAATTCCCAAAACACCTCCCCCTACCTGCATTTGATTAAACCTAAAAAAGTTACTGCTTCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGGGTGAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAACCCCAACCCCAAGCCCTCAAAACAACGACACCGGTGTTTGGTACGAATAGTGGTAACCTCGGTAGTGTGCTTAGTGATGGGGGTACCACGCAGGACTCAAGCACCACCAATCAACTGTCACCCGTTGAACGGGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGAGGTGGTGGGGGTGGGAGATTTGTCTAAGCGAGCTTCCATTGAATCAAGTCGTTTGTGAATAGCATTAAAACCTTAGCTAACGCTAACTTTGGGCGATTTAATCCACTTAGTTTTGAACTCCTTAAATTTGTGTTTTTCTTGACTTTGCTAAAAAAACAATTCAAAGAGGCTTGCAATGAACAGGTCAGCACTGCTAAATTAGTGAGTGCAAGAACTAAATAAAAGTAATTATTTTGTAATTTTTCGGCGGTCTTACCATACATCAATTAAATGCTTGATCAAAACCAAGGTGTCAAAATTTAGTGCACAATTGCTAAGAGCGCCTGTATATTTAGAGTAAAACTACTTAAATTGCTAAAAATTAACCGTTTTTGAATTAGTTTTAGGGTCAAAATTAGGCCAATTTCCCGCTTTCTTTTTTTAAAAAATCTCTACTTTTTACCCCCTAAATAACCTAACAACTACACAATTAATTCCCAATTAATGTAAATTTATCTAAATTGTTCACCGTAGTTAGTTCAATTTAGGTAAAGATGAAAGGGTTTTCTTGCTCCAGACCGGGCTATCTTACTGGTCTCTTGCTCCTCGCTGTAGCCCCTATTCTCACTGCTTGTACCCGCGACTATACTACCAAGAACGAATTTCAATTAACAACAGCTCAACAGGCCAAATTAAAGCCAGCCACCATCGAATATTGGCGTGATGGTGATACGCCTGAGATCAATTACGCAAGCGAAGAGCGCCGAAAGGAAGCTGAACAAAAATCTAAAGAAAACGCTAAGAAAGAAGACAAAAAAGAGGAAAAGAAAACTGAAGATTCCCAAGATTCTTCTTCTGCATCCACGCAAGTTCGTAGTAGTAAACACGGTCTCCGCATCTATGGAATAGATACACCCGAAAAACATGTAAGTAGCAAAGGCGATAGCACCGGTGATGAAAAAATCGAAGCCGAAAAGGCCTCTAACTATGCTGAAAAGCTAATCCCTAAAGGCAGTACTGTTTGGGTTTGATCACTCAACACTTACAGCTATGATCGTGAAGTGGGCGCTTTATTCTTTAAGTCTAACCCTAAGCAAACCTTCTTCCAATCCTTTGAAGTAGCTATGGTTGAGGCTGGTCATGCTATTCCAATTGCTGGGACTGGTTTAAATTTAATCGCTGATCCGGAATTAAGTGCTGATGATCCTTTAAGTGTCATTGGTCTACAGTTAGCCAACGCTGCCAATAAGGCTTACAACGCTAAAATTAACATTTGGAGCCATGACACTGATGGTTATCGTTCCTTGACTGCTGTTTACAAGTTAAGGGGCGCTGATATTAGTTGAACCCGTTTCCTAGATGAAGCCAATGGATATTCCTCTGCTAGTGCTGGAACCGGTGCTAGTTTGTACCAACTCTGAGATCAACGTCAAGCCAAATTAGCCCAAAAGGGTAGTTAATGAAAGCAGTTTAAACCAGCAATATGGAAAAGATACAAGCGGAAAAATCCCAAAGTGCAATTGAGTTTAAGAATATTGTAGTCGACTTTGGTGAGTCAATCGCAATTGATAACATTAACCTCACGGTTAAAAAGAAGGAGTTAGTTACCTTACTAGGTCCTTCTGGGTGTGGGAAAACAACTTCGCTTTCCGTGATTGCGGGTCTTATCGCCCCTACTAGTGGTCAGGTTTTATTTAATGGTTATGATGTCACAAAAAAGCCACCACAACAACGCAAACTGGGATTAGTGTTCCAAAACTATGCCTTGTATCCGCACATGAGTGTGTTTGAAAACATTGTCTTCCCACTCTACAGTGATACGTCTTGACGCGAAGCCATCTTTGAAAAAAATACCTGAGCGCAACACGACATTAATTGCTTGATCTTAAAGGCTAATGGTGCTACTAGTGAGGAGCTGGCAGAGCTCAATCGTTTAATGCAACAACGAATCGATGAGCCCAAACGAATGGCTTACCAGATTAATGATTTGATGGTAAGTGTGTTTCAAAAACAGAGTGAACTGGAAGCTAACCTAAAGTTAATTCCCCGCAAAAAACAGTTTGCCATTATTTCACTCAGCAAGGAAACACTAAGTCAAATAAGGGATGTTGAAACTAAAGCCAAGGCTGCATTGGAAACCGCTGATAGTGCTGAAGTGGAGCAGACCATTAAGAGTGAACTTAAGCAAAAACTAAGTGAAATTAAGGCTAATTACCACGATGAAAAGGCCAACATTAAAGCTTACTGATGGGAAATGTTGGCCAATATTAAGACCGAATTAAAAACCGAAAAAACTGCCATTAAGCAAACTAATGACTATGCCAAATTGAAGGAATTGAAGTGAAAGATCCACTTTGAACCCTTGAATTTAAAGAAGCAGTACCGCAGTTACTTTAAACAACTAAAAGCCAAATACAGCTTAAAAGATGGCAATTTAACTGAAAGCGAATTAAGTCAAATTGAGGAGTTGCAAAAACGGATTGTTTCGCTCAAAGATTTCATTAACCGCACTGCTAAAGAAGTAGCTGAAAAACTGGAAATTACTAAGATTCTCCACAAGCGCCCCGCTAATATTTCTGGGGGTCAACAACAACGGGTAGCTATTGCTAGAGCAATTGTTAGAAGACCTAAGGTTCTACTTATGGACGAACCGTTGTCCAACTTAGATGCGAAGTTGCGGGTACAAACACGGCAATGGATCCGTAAGTTCCAGCAAGACCTGCAAATTACTACTGTCTTTGTGACTCACGATCAGGAAGAGGCAATGAGTATTAGCGACACGATTGTCTGCATGTCAACTGGTAAGGTACAGCAAATAGGCTCCCCTTCCGAGTTGTACTTAAAACCGGCCAATGAGTTCGTTGCTACCTTTTTGGGTAGCCCGGAAATGAATATCGTCAACGCTACGGTTAAAGCTGGACAGCTGTTGTGAAACGAAAGTCCCCTAGTTAAAACAAAGTTTGATTTACCTGATGGTGCCATTCGGGTTGGTTTCCGTTATGATGAGGTAACGGCTCCCAAAAACGATGGTAGTCCCGTATTTAGCGGTACCCTTATAAGTGTGGAAAACCTAGGGAAACACATGGTTGGGGTTGTGGAGAGCAATGGGGTACAGCTCAATGTGCGCTTAGAATTGAGCCACCAGTTTGAAGTAGGTAATGCAGTTAAGTTTACGATTAAGCCTAATGGCTTACACTTCTTTGATCCCCAAACAACGCAACGGGTGGAGGTAAAACATGTTTAAGTGATTGTTAAAGCACCGCTCTAAACCAACGCCATTGGAGCTGGGAATCTTTGACCAAAAGGTTTCCTTTTGAAAACCATTTTTGCTGTTTTGCCCAGCTTTATTAACTACCTTTCTCTTTACTTTAGTACCGTTCTTCCTTACGTTACAGAAAGGGTTTAGTCACAATGAGGACATCTACCGGGTTGATTCCCAACAGTTTGGCTTTCAAACTTTCGCTAACCTTTTTAGTGAGTCCAACTTTATTTTGGGCTTGCGCAATTCCTTTTTGTACTCGATTATTTCCCTACCACTGACAATTGTGCTAGCAATTATTATTTCTAGTGCCATTGTCTTTGTGTACCGGAAGTTAGCACGGGGCTTTTGACAAACAGTATTCTTTTTACCCTATGTAACTTCCGGAGTAGCGGTTTCGATTGCTTTTATTTATATTTTGGACTCCTCTTCGGGAATCTTAAACAACATCTTTCATGTCAACATTAAGTGGTTAGATTCGGGGGAACGTGATACCTTTAATGCCTTATGGGGAATTCTTATCTTTGGAATTTGGAAGAACATGGCTTTTAATGTGCTGGTAATATCAACCGCCATGTTAAGTGTGGATCCTACTTTATATAAGGTAGCTAACTTAGATGGTGCAAAACCAATTAGGCAGTTCTTTAAGATTACCTTACCTTCAATCCGCCCAACCTTAATTTTCCTGTTGACCCTGTTAATTTTGGGCGGAATGCAGGTGTTTCCAATTTCGTTGTTTAACGGGAATGATAGTGAAGCTGTAACCAATGGGGGTTCGACCATCTTGCTTTACATCTTCCAAAAGATTCGTGATCAGAATAACAATTTTGCTGGTGCTGCTACCTTGGTGTTGTTCATCTTGGGGGTTTGTTATGGGTTAGTGTTGCGCAATGGTTTTCGCTTAATTGAATGAGCGCAGTGAAAGATAAAGAGACATTATGTTCAAACAAAGCTTAATCTGGTATAACCGTTACGTCCAAAAACGGAAGAAGGGTTTAGATCTCACCATTAAAGATCGATCTTGATCGAATGTATTGTTGGGTTTAATCTTTAAAACGGTGGTGTTGTGCTTCTTTGGTTTAATGGTCATCTTTCCCTTTTATTTGATGCTGGTGGTAGCACTCACCAGTGATGAAGTGGTCTTAAACATTCGTGAACCAATTCTCAAATCCGATGGGTGGCACTTCGAGAACTTCCGCCGTGTTTTGGAAGATGGTAAATACCTTAATGCCATTTGGATTAACAGCTTAGTAACAATTTTGTCGATTATCCTACGGCTTTTCTTTACCGTTAGCATGGGCTATGCCTTTTCGTTAAAAAAGTGAAAGCTCAAGAAGCTGTTTTGGTTTATCTTTTTGGCAGTGTTAATTTTACCCGAATCGGCCTTGCTAATTGGTCAGTATCGTGTGGTGGTAGTTGCCAATTGAAATCAACCAGAAAAACCAGCGATTATCTTGGGCTTAACAATGCCATTTGTCGCTTCTGTGTTCTCTGGGTTTATGTTCCGTACTGCCTTTGAAGCAATTCCACCACGAATTAAAGAATCGGCCTTTGTTGATGGTTGTACAGGGTTGAGGTACTTCCTCAAAATTGCCTTTCCAATGGTACGTAGTACCACTTGAACGGTAAGTATTTTAACGGCCTTTGCGGCGTGAAATTCTTACTTATGACCGTTATTGTTACTAACAAACAGACCCGATCTCAACATTAACCTGTGGGTGTTGGCGCAAGGGACTGATGGCAATGCCGGGCAAAGTGACGAACAAATCCGGGTGTTGTTGAATTTAAAGATGGCAGCAGCCATTTTAGCAATTCTGCCAATGTTTATTGTTTACTTCTTGTTTAGGAAAAGGATTATGAAAGCCGTGGGATCACGTGCCAATACGATTAAAGGATAGTTATGGACAGCTTAAAGCATTTTTGACAACAACTAGTAATGTGATCAAAAACTGTTACCTTTTGAATCATAGTAGCAGTAGGGATTGCTTTTTGTGCCTTTGCCATTTGGGGCATTATTGCTTGCATTGAAACGATTATCAAAGAAGGGGCGTTGTTGTAAGTTAGCTTTCTTAGCTTTTTTATAAAAACACCAGATTGATATTTAGCCTTTTTGAGTTAGCTGTTCTCACTGCTTTTGGCGGTTGCTATACAGAATGAAGGTCGTTTGTAAAGGAACGTAAACTTTGCTAACTTAAAGATCTCTTTTTGGGTTTTAGGTGTTTTATTTTTGTTACATAAAATGAAGAAAAGGTTAGGAAACTACTTGCAATCAAAAAAAGCGTTTTAGGTAAAAAAAGTGCTTTGTTTCATAACCACCCTTCGAGGTGGTTTTTTATTTGTTTTATAGCTTTGTATACGAATTCACTCTATAGATATAGGGTGCTTTTATTTTCTGGCAAGCTGAAAAGGGTAAAAAATACCAAAAGTTAGCAGTTAAGAAACCACTAAGAATTGGTATTTTGGTTTTACTAAATACTATTTGGGTGGATCGATTTTGTCTAAGCGAGTTTCCATTGAATCCAAGCGATTTTCCATAGAGTCTAAACGTCCTTCAACAGAATCAAGGCAACCTTCTATTGAATCCAAGCGATTTTCCATAGAATCAAGACGACTATCCATAGAGTCTAGACGACCTTCAATAGAGTCGAAGCGTTTATTGGTGGATTCTATACTTTCAACCAAAAGGTTTTCCAAACGATCCATGCGGGCGTTAAACTCTTGTCTTTGCGCTTTTTGCTCCACTTGAAGTTCTCTAATTTGCTCGCCCTGGATTCTTATTTGTTCACCTTGGGCAGCCAAAGTGGTTTCAATCTTTATGAGTCGTTGGTCGTTTGAGTCTTTGTACTCGTTAAACTCTTTGCGCGTTACGTATTCCGTGTTCACTGGTATAACCGGTTTGTTAAGTTTAAATTGTCTATTGAAATTGCCAGAGAATTCCACACTTTTGTTTTCTGGATTAATGATGTATCAGCCCGAAAAAGTGCCCTTTTTGGTCTTTTTCATCATCTCGTTAAATTCCTTTTCGTTATAAAACGGAATTTTCTCTTTCATTAATAAGCGACTCCTTTCTTTTCAGTGAAATTTAAAAAGTAACTACTACTTGCAACTCAGTTGCCAACAGGGATCAGACCATTTACTGATCAATTTAAAAAATAAAAGATTTGCATTTTGCTCCTCCACTAAATAAATTGACCGGCTTATTGAGAAGTGCCAACAAAATTGCAAAAAATTTCAAATGAATAAAAATACCAATAGCAATTTAGCCATTGGTATTTTTTAGTCTACAGTTATTTAGGCGGATCGATTTTGTCTAAACGAGTTTCTATTACGTCGAGGCGTTTACCAAAATCAGTCAAAGTGTTTCCGATTGATTCTAGACTTTTAAGCACAAGACTTTCAAAGCGATCCATGCGGGCGTTCATTGCTTCACCCTGGGTTTTGATTTGTTGACTCTGAACCATAACAACTTCAGTTAGTCGATCAACTTTGTCCTCAACAACTTGAATCCTTTGACCCTGACTCTTTAACTCAGTTTCAATCTTTGTAAGTTTTTGGTCGTTTGAGTCTTTGTACTCGTTAAATTCTTTACGCGTTACGTATTCTGTGTTCACTGGTATAACTGGTTTGTTAAGTTTAAATTGTCTGTTGAAACTGCCAGAGAATTCCACACTGTTGTTATTTTTATTGATGATGTATCAACCCGAAAAAGTGCCCTTTTTGGTCTTTTTCACCATGTCATGGAATTCTTTTTCGTTATAAAACGGAATTTTCTCTTTCATTAAGCAATAACTCCTTTCTGATTAAAGCGGTTCAAAAAACTACTACTTACAACTCTTTGTCATTGGACTTTTCAAAATTTGATCATTGTTTTAATGCTCCTTATTAACTAAATTGACCCGCTTCATAAAAACTGCCAACCAAGTTCAAAAAAGTTTTTAAGCTATTTTTGCACCAAAATGGCGTTGTCAGTCAAACTACGACAACAACAGTTGCTGTTTGATTCTTTAAACTTAAACAGAATTAGCACCAATGAATAGCCAAGTACACCGCAAGGGCTCTATTGCAGAAGCAGTTAGTGCCATCCAAGCTCATGATAAGATCGTGATCTTCCACCACATTCGTCCTGATGGCGATTGTTTGGGCGCACAACACGGCTTAGCGCGTTTAATCCAAACTAACTTTCCCCACAAGCAGGTCTTCTGTGTTGGTGATCCCAAACACAACTTTCCCTGATTGGAGATGGTTTTCACTCCAAAGGAACAGATTACCCCGGAGTTAATGCAACAAGCCTTAGCCGTTATTGTTGATGCCAACTATAAGGAACGGATTGAGTGCCGGGACTTATTAGACCAAAACCAGTTTAAGGCAGTATTGCGGATTGACCACCACCCCAATGAGGACGATCTCAATACGACCCATAACTTCGTTGATGCGTCTTACATTGCCGCGGCTGAACAAGTGGTAGATCTAGCGGTGCAGGCCAAATGGAAGCTTAGCCCCCCAGCGGCTACGGCGCTGTATTTAGGTATTTATACAGATAGTAATAGGTTTCTATATAGTAATACATCATGAAGAACACTATATCTAGGATCTATGCTATATAGAGCTCAAGCTAATATAGCTAAGATCCATGATGAGTTAAACCACACTTCCTTAAAGGACATCCAGTTTAAACAATATGTCTTTAAAAACTTTCAGACCTTTCAGAATGTTATTTACTTTGTGGCCGATAAGAAGTTCCAAAAGAAATTAAAGGTAACACCCTTAGAATGTGCACGGGTAAATATCCTAGCTAACATTGAACAATTCCACATTTGGCTGTTCTTTATAGAAGAGGGTAAGAACCACTATCGGGTCGAATTCCGTAGTAACGGAATTAACGTACGCGAAGTAGCTTTAAAGTATGGTGGCGGGGGTCATATTCAGGCCAGCGGTGCAGTTCTTAAAAGCAAGCGCGACATAATTCGTGTAGTTCAAGATTGCCAAAAGCAAATTGCTGTATAATTTTTAACAACTATGCACCAAACCAAAAAAACTGCCTTGTCCAAGTCCACTTGGATTCTCATCCTCACCGCCACCGCCTCCCTCGCGACGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTAACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGAACGTATCGTAACACGAGCTTTTCCTCCCTCCCCCTCACGGGTGAAAATCCCGGGGCGTGGGCCTTAGTGCGCGACAACAGCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCGCATCAACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCGCAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCGCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGTAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCAGACAGCACTAACCAAACAGGCGCAATGTTTGGCTTGAAGGTGAAGGATGCAACCGTGGATAGTTCGAAGCAATCAACGGAAAGCTTAAAGGGCGAAGAATCGAGTTCCAGTTCCACCACATCTTCCACCTCCACCACCCAACGTGGGGGTTCGTCAAATGAAAACAAAATCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGAACGCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAACCGTACCGCCATTGACCGCGTTGATCACTTGGATCCCAAGGCCATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAATACCCAAACCGGGTTTGATACAGATGACACCGATAATAAAAAGCAGGGCTTTCGCAAACAAGGCGAACAATCCCCTGCCCCCATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGTGGCAATGGCCATGTTACCAAGTCGGCCCACACCGCGCCTTTGAGTATAGGTGTCTTTAGGGTGCGCTATAATGCAACTGGTACCAGTGCTACTGTAACTGGTTGACCATATGCCTTACTGTTCTCAGGCATGGTCAACAAACAAACTGACGGGTTAAAGAATCTACCCTTTAACAATAACCGCTGGTTTGAATATGTACCACGGATGGCAGTTGCTGGCGCTAAGTTCGTTGGTAGGGAACTCGTTTTAGCGGGTACCATTACCATGGGTGATACCGCTACCGTACCTCGCTTACTGTACGATGAACTTGAAAGCAACCTGAACTTAGTAGCGCAAGGCCAAGGTCTTTTACGCGAAGACTTGCAACTCTTCACACCCTACGGATGAGCCAATCGTCCGGATTTACCAATCGGGGCTTGAAGTAGTAGTAGTAGTAGTAGTAGTAGTCACAACGCACCCTACTACTTCCACAATAACCCCGATTGACAAGACCGTCCAATCCAAAGTGTGGTTGATGCCTTTATTAAGCCCTGAGAGGACAAGAACGGTAAGGATGATGCCAAATACATCTACCCTTACCGTTACAGTGGCATGTGAGCTTGACAGGTATACAACTGGTCCAATAAGCTCACTGACCAACCATTAAGTGCTGACTTTGTCAATGAGAATGCTTACCAACCAAACTCCTTGTTTGCTGCTATTCTCAATCCGGAATTGTTAGCAGCTCTTCCCGACAAGGTTAAATACGGTAAGGAAAACGAGTTTGCTGCTAACGAGTACGAGCGCTTTAACCAGAAGTTAACGGTAGCTCCTACCCAAGGAACAAACTGATCCCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTTGTGGGGTCGGTGCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGTACAGGTATGGCAATAACCACCGGGGCGTGGATGATATAACCGCGCCTCAAAACAGCGCGGGGTCGTCCAGCGGAATTAGTGCGAACACAAGTGGTTCGCGTTCCTCTCTCCCGACGTTTTCCAACATCGGCGTCGGCCTCAAAGCGAATGTCCAAGCCACCCTCGGGGGCAGTCAGACGATGATTACAGGCGGTTCGCCTCGAAGAACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTTCAAGTGGACAAAGTGACGACCACACCAAGTTCACGAGCGCTACGGGGATGGGCCAGCAGGAACAATCAGGTACCTCCGCGGGGAATCCCGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACAGCAATGCGATGGATCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTTAATAAGTCCGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGAGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAGTGGCACAAACACAACGTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCGCAAGACGTTGGTAACCTCGTTGTCAGTGGCACCAGCTTCAGCTTCCAGCTCGGCGGGTGGTTAGTTACGTTCACGGACTTTATCAAACCCCGCGCTGGTTACCTCGGGCTCCAGTTAACGGGCTTGGATGCAAGTGATGCGACGCAGCGCGCTCTCATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGTTGGGCCGCGTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAAGGGACGCCTTACCGGAGCACCCGAATGCTTTGGCGTACCAAATTAGCTATACCGACAAGGATTCGTACAAGGCTTCCACTCAAGGTTCGGGTCAAAACAATTCGCTCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTCGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTGTTTGGAGCCATGAGTGGTAACCTCAGTAGTGTGCTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAAAAAGTGAGTGGGTGGCTTGTGGGGCAGTTACCAAGCACGAGTGACGGAAACACCTCCTCCACCAACAACCTCGCGCCTAATACTAATACGGGGAATGATGTGGTGGGGGTTGGTCGACTTTCTGAAAGCAACGCCGCAAAGATGAACGACGATGTTGATGGTATTGTACGCACCCCACTCGCTGAACTGTTAGATGGGGAAGGACAAACAGCTGACACTGGTCCACAAAGCGTGAAGTTCAAGTCTCCTGACCAAATTGACTTCAACCGCTTGTTTACCCACCCAGTCACCGATCTGTTTGATCCGGTAACTATGTTGGTGTATGACCAGTACATACCGCTGTTTATTGATATCCCAGCAAGTGTGAGCCCTAAAATGGTTCGTTTAAAGGTCTTGAGCTTTGACACCAACGAACAGAGCTTAGGTCTCCGCTTAGAGTTCTTTAAACCTGATCAAGATACCCAACCAAACAACAACGTTCAGGTCAATCCGAATAACGGTGACTTCTTACCACTGTTAACGGCCTCCAGTCAAGGTCCCCAAACCTTGTTTAGTCCGTTTAACCAGTGACCTGATTACGTGTTGCCGTTAGCGATCACTGTACCTATTGTTGTGATTGTGCTCAGTGTTACCTTAGGACTTGCCATTGGAATCCCAATGCACAAGAACAAACAGGCCTTGAAGGCTGGGTTTGCGCTATCAAACCAAAAGGTTGATGTGTTGACCAAAGCGGTTGGTAGTGTCTTTAAGGAAATCATTAACCGCACAGGTATCAGTCAAGCGCCAAAACGCTTGAAACAAACCAGTGCGGCTAAACCAGGAGCACCCCGCCCACCAGTACCACCAAAGCCAGGGGCTCCTAAGCCACCAGTGCAACCACCTAAAAAACCCGCTTAGTATTTATGAAATCGAAGCTAAAGTTAAAACGTTATTTACTGTTTTTACCACTTTTACCGCTAGGGACGTTGTCACTAGCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGCGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGGGATACCAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGATCAAAGTAATACCAACCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTATGTCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGATCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGACCGGTGTATGAACCCCTGGATTCGACGAAGAATGGCAAGGGGAAGGATAAAGATGGGTGAACCACTTCCGGGGCGAGTGAGGCCAAAGGAGATGCCCCGTTGGTGAGCTCAACCGAGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGGTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCTACAAATGAAGATCTGCCGAATGTAATAACACAGTTATACCACACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCCCACACCGAGTTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGAGCACGGAAAACAGTGCTGCCACCAAAGACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGAACAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGAACGAGGAGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCACGGGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGAACCAACACCGCCTCGCACTACCTCCCCGTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGAAGGCGAGCGGAGCAGCGGAATGGGGTGAAGATTAGCACCTTGGAATCGCAAGCCACTGATGGCTTTGCCAACTCGTTACTTAACTTTGGTACCGGTCTTAAAGCCGGTGTTGACCCAGCTCCAGTAGCACGGGGTCATAAACCGAACTATAGTGCAGTACTACTAGTGCGTGGTGGCGTTGTAAGGTTAAACTTTAACCCCGATACTGATAAACTGTTGGATTCTACTGACAAAAACAGTGAACCTATCTCCTTCTCCTATACCCCATTTGGGTCTGCTGAAAGTGCCGTAGACCTCACCACGTTGAAGGATGTGACCTATATTGCTGAAAGTGGTCTGTGGTTCTATACCTTTGACAATGGTGAAAAACCAACGTACGATGGTAAACAACAACAGGTTAAAAACCGCAAGGGTTATGCTGTGATTACCGTATCACGTACCGGAATTGAATTTAACGAGGACGCTAATACCACAACCTTAAGCCAAGCCCCAGCTGCTTTGGCTGTCCAAAACGGGATTGCTTCCAGTCAGGACGACCTCACAGGCATCCTACCGTTATCCGATGAGTTCTCCGCTGTGATTACCAAGGATCAAACATGGACCGGTAAGGTTGATATCTATAAGAACACCAACGGGTTGTTTGAAAAGGATGATCAGCTATCGGAAAACGTGAAGAGGCGTGACAACGGTTTGGTCCCTATTTACAACGAAGGTATCGTCGATATTTGGGGCAGAGTGGATTTTGCTGCCAACAGTGTTTTGCAAGCGCGTAACCTCACTGATAAAACGGTTGATGAGGTGATCAATAACCCCGATATCCTCCAAAGCTTCTTTAAGTTTACCCCAGCCTTTGATAACCAAAGAGCAATGCTAGTGGGGGAAAAGACATCGGATACTACCTTAACGGTTAAACCGAAGATTGAGTACTTGGATGGTAACTTCTATGGTGAGGATTCCAAGATTGCTGGAATTCCGCTCAACATTGATTTCCCTTCCCGGATTTTTGCTGGCTTTGCTGCTTTACCGTCCTGGGTCATTCCGGTATCAGTCGGTTCATCGGTGGGCATTCTCTTAATCCTGCTCATCTTAGGCCTTGGTATTGGAATTCCAATGTATAAGGTCCGCAAGCTTCAAGACTCCAGCTTTGTTGATGTGTTTAAAAAGGTGGATACGTTGACAACCGCTGTGGGTAGCGTGTACAAGAAGATTATCACCCAAACGAGTGTGATCAAAAAAGCTCCTAGTGCGTTGAAAGCTGCTAATAACGCTGCTCCTAAAGCACCAGTTAAACCAGCTGCTCCAACAGCTCCAAGACCACCAGTCCAACCACCTAAAAAGGCTTAACGATTAAGCCCCAACAGAAAGAAGCACAACTGTAAAAAGGTTGTGTTTTTTTTCATATTTACAACTTTTTGCTTTTTTGTTGGCACATTACCTTCCTAGTGCAATTTAGAAGGTGTATGAGTCATAAAGATTTTAATGGGCTTCAAGCCCCTCAGTTGTTAAGTTCGAGTTCCCCTGTTGCAAAGAAACAAAGTAGTCATAAACTCAGGCATGCCTTAAAGCACGCCCGTTACTTAAACCATAGTAGTAAGCGTACCCTCAAGCATGCCTTAGAGCTGCATGAAGACAACCAGGTCTTACTGGAAAAAGAAGGTAGTCCTAACTTCCAGGACTGGCTTTCTAAGCAACCTGGCGTCAATAAAACCAGCCTTAAGTACAACAAAAGCTTGGGTAGTTGAATCTCTAAGGAAAGTAAACCGAAAAAGCGTTTTCAACCGTACTTTACGTACAAGGGCAGTAAAACTACTCCAGAAGAGGCCAAAGCCTTGCAGCAAATGAAACAATCCCAAAAGCGCTTCTTTCACGAAAATATGCACAGCTTTCTCAATGAAGTTGCACATAACCCAATGATCCAGCGCTTTAATCAAAAGCAGGCCAAACGGGCTGCTAATACGCGACAACGCACTTACAAATACCGTCAGTAACAATGCACAAGATTAAACACTATAAACCCTTAAAGCGCAAGACCTGGTTAAACAAGGTTTCCAAGCAACGCCGCGAGCGCATGTCCGCTAAGTGCTATCAACCACCTAAGAGAGAAGTGATGCAGCCAACTTGAAAGAGTTAGCACCACCTGATCCCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTTGTGGGGTCTGTTCTGGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGCGTGGATGATATAACCGCGCCTCAAACCAGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTTTCCCGACGTTTTCCAACATCGGCGTTGGCCTCAAAGCGAATGTCCAAGCCACCCTCGGGGGCAGTCAGACGACGACTGTGGGCGGTTCGCCTTGAAGAACCCTCGACCAAGCTAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTTCAAGTGGACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGGATGGGCCAGCAGGGACAATCAGGTACCTCCGCGGGGAATCCAGACTCGTTAAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAACCACGCAGGACGGCAATGCGACCGGTCAACAAGAGGCCACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCATCGCGCCCAGCTCTTCCTTCGCGGCTTGTTGGGCAGCATCCCGGTGTTGGTGAATCGAAGTGGGTCCGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTCGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGCTCGGCGGGTGGCTGGTCAGCTTCACGGACTTTATCAAACCCCGCGCGGGTTATTTGGGCCTCCAGTTAAGTGGCCTGGATGCCAGTGACAGCGACCAAAGGGAGTTAATTTGGGCCAAGCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGCTGGGCCGCGTGGAGAGTGTGTGGGATTTAAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTGAAGCTTCCGGGTCAGCTTTGGCACCGCACCCGAATGCTTTGGCGTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAGCTCTTCAACCTCAAGTTCGGGTTCCGGGTCAAGTTCAAACACCTCCCCCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCTAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACATTGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGATCAATCTGGTGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAACTATTAATAGGAACTTTTCTTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACCAACTTTAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGGCTTCACCTCTTTTTAAAACAAGTTCAATTGCAACTTGTTCCTTTGTTTAGCCAAAACTTTTTGCAATTTTGTTGGCAGCTTTAAATAAACCGATCAATTTATTTAGCAATGACACAAAAATTAATATGTTGAATTTTTTAAATGAATCAACAATGGTATTGATCCCCGTTGGCAGCAGTGCTGCAAGTATTAGTTACTTTCCAAACCTTACAAAGAAAGGAGGTGATTTTTAATGTCTTACTCACCTTCACTTAAGGAAATTATTGCTATTTTGCAAAAATACACTTCGAAAAACTACCAGTCAAATTGCAAAACTAGACCCGATGGTAAGCTTGAATTATCATTAAATGGAGTTTTTGAAGAAATCGTGAAAACACCCGGTAAAACTAGGTACGTAACCCATAAACAGCTTGATCAGAAATTAAAAGATTTCAAGCAAGATCTGATGGTTGAGTTGCACGACACGTTTGCTACTAAAACTGATCTTAAAGAATCGGAAGCACGCATTAACCAAAAGTTAGAAGCACTAATTCAAATTGTTATGGTTCAGGGTGAGCAGATAAAAGTTCACGGCGAGCAAATTAACAAACTGACTCAAACTGTTGAAAAACAGGGCGAGAAAATTGAAGCTCAAGGACAACAAATTCAAAAAGTTAATGAAACTTTGAATTTTGTAGTTGAAAGCCTTGGATCTATCCACAAAAGACTCGATTCAATGGAAGGCCGCTTGGACTCAATGGAAAACCGTCTTGACAAACTAGAGTCCAAGTAGTTCTAATGGAAATAAAAATTCGAGCAGTTATTCTTAGAGATTAGTTGCTGGTATTTTTATTCACTTTGAAGAGAAAAATCATTTGTTTAAACTCAAAAAGGTCAATAATTTTTAAAAAATGCTGAGGTACAACCACCTTTTAAAAATTCCTTAGATATAAATCGCTAAACTATCTTTATTTATTTTGTATGTGCCAAATTATTTTTGACACATCAAATCAAATATTATTTTTTATCATTCCCCTAAATTTCAGGGGTATTTGAGTATGCGATTTAAATATGGTGCAGTTGCTTTTAGTGGCTTTTTAGGTCTAGCAGTGATGTTGGCTAGTTGTGGTGCTAAAGGGCGTTTTGACCAGGTTGATGATGGCAAAATTAAGCTAGCTTCTTCACTTACCGGTAAGCGCGATGTGATACTGAAAGAAGTGCTCAACAAATATAACAGTCGCAAAGCCAAGGACGATTATCCGATTGAAATAACCAAAATAGCCGGTAGCTATGATGGTGGTCGATCTGATCTGCAAACGCGGTTGAGTGTCAAGGATAAAACAACCTTTTACAACATGATCTTGAATTATTCTGACACCATTTCTACTTTAGGTCGCAGTAACATGGAACTACCATTAGATAGCGTTGACGTATCACAGTTTTCAGAAAACTTCTTGAGCTTTAATGACCGCATAAGTGGTATTTCCAGAAAAGGCATTTACGGCATTCCGGTATCAATGTCAACTGATATCCTAGTAATCAACGGGCCGGTATTGCACTATATTTTGAATAGTGCAAAAAAGAAAGATGGTGCAGTAACTAAGAAAAACGCAAGTAATTCCAATGGTAACGAAGGAACGTTAACAGTAAATAACGATCAACAAACCACTGAACTATGAAAAAAGATTGAAGAAGCCGCTAAAACTAACGGTAAAACTACCCAAGAACAAACAAAAAGAGATGCAAAACAAAGTACATCCTTAATCCAACTAAAAGAAGGATCTGCAAATACAACCGAAGGCAATGCTTCAGAAAGTGATAAAGAAATCAAAAAATCTTGAGGCAATTACCAAGAAGTAGATGGTGGTCTTAAGGGCTATACCTTCAAAGCAAGCGTCTTTGAAAACTAAAGTGAAATGGTTGATTTTGCCACTAGGGTAGCGAAATCATTTAAGGATAAAGTTAGTGGTATCGATAATAAAAAAGGCACTGACATCCAAGGTGTTTTAGGTCTAGACAGTACACCAAACGTTTTGTTCGCTTCAGTATTTGCAGCTGGTGGTGGTAGCTATGACAACTTCTTTTACAAGGTAGAAAATGGCCGGGCTGATTTCCGTAATTTTGCAAATAAAGGTACTTCTTACAAAAACTTAGAGAAGGTTTACAACGATTACAAGAATTTAATCGGTAGCAACGGTCTTTTTGCTAGCAAAGATGGCTCTTACTCATCTAACTTTGAAAAGTTCCACCAGTTAGCTTTTTATGTAGGTTCTAGCTCTGGTTATAACTATGCTTTTGCTGATGAAACTGCTAAACGGTTAAAGTTTGGTGATAGTGCCATTGAGTATCCTAACGATACGCTTGAAATTAAGGCACCGACAAATAATAGTCAAAGTGGTGATGGGAATGGTGGCACCAATAACGACAATCTCTTAGGTACTTTTGACATTAGGGAAAAGAGTAACGGCAAAAAAGGTGAGAGCAACGGTAAACAAGGAAATGGTCAAGATAAAAAGACAATTTCGCTTTATAAAGATTCGATTCCTAAAGAAAAAACTGAAGGCACTGATGCAATTCTAATTAGCGATAATCAACTCATTAATCAATTACAAACTGCCGCTAAAACTAGTAGTTCACAAAATAAAGCTAACACTGCAGCTATAACTTTTAAGCAAAGCAATAAAAGTGAAACTGACCAAAGTACAACTAGTCAGTTTATCGGCTACACAACAACTGCTTCATTGAAAGCTGATAAGAAAAATATCTTTGATGTGAAGAAGCTAAATAACGAAAAGTCTGAGCGCAAAATCATTGTCGGTGCCACGGTGGAAACCTTAAATCAAGCAAACACATTGCAAGCTAATGAAGCCATTATTAAACCAGCACCTGGTAAGTACCAAAGTACTGATTCACACAAGGTCATGATTACTCAAGGCCCGAATATTGTCGGAATTCATGCCAACGAAAAAGAGGACAAAGAAACACAAAAGTTTATTAACTGATACCTCAATAAAGAAGAGAGTTGATCTGTGCAAAACAGCGGTTCAACTACAACTAAAAAGCAAACTGCTGCTCAATATTTTGCCGAACAGTCCTCTTACATCACTCCTTTAAAAAATAACTTTAAAGCAGATCAAAGTGCAACAAAAGATAGCACTGTTAACACTAACTATTTCACTAAACAAACATTTGATCTCTTCAAAGAAGTTAATGATGGAAAAGTGCTTGGTTTTAATGATCCAAGTGACTTTAGGAGTGGTAAGTTCCGCAATACTATTGGTTCTACTTTTAACGCAACCATCAGTTCTAAAGTTGACTTTAACAAGTTTTTTGAAAACTTTAAAGCTTCTTTAGGGTCTGGTTTTGAGCGTTAATTTTAAAGAAAATTTTTCTTTTCAATTGCGCTTAATCTAAAGGGCAAAAGTAACAGCAGTTCACTGCTAAGGTTGATTGTGTTAACCCGAAATTTCAATTAATTTCCTTTTCCTCAAAAGGTTTTCCAAAAAAAAGTAAAATAGAAAAGCTATTTTCACTTTTGATGCACAAAAAGAAGTTTTTATCAAGAACTTCCTTTGCTTCTTTGTTTTTGCTTTTAGGAACAGTATTGAGTGCTTGTAGCAAAGTAGAGTCCACAAAAAGTGTTCATCCTGTAAAAAGTTTTAAATCTGATCTAAAAAGTCTTCTTAAAGAAACAATTAAACAAGATATTGATTTATCTAAAACAATCAAAACTTCAGAAGGCAAACAACAGATAATAACTTCTTTAAAAAAGTCTTACGGAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCATGAAAACAGAGCTTTGAAGAAGGTGAAATAGGTATTGCGGATCTTGATTTTGAAGTAGCTTATCCCAAAACCCAAGATCCTTTTACAATGGAGCGAAAGGTTGATCATTTCCAAATGACATACCAAAGTTTTAAGGATTTATCAATTAAAGCTAAGTTGAGCTATACCTTTAATTGATTTGGAGATTATTCTTCAGGTGGTTTCACTGCTAAGAAAGGTGACAAGCATTATTTCGATCTTTTCCTTAAAATCAAACCTGATCCCAACAAATCATTTAAGGCTGCAAATTTTAAAACTGAAGAAAAAAACTCAACAGTTATTGATGGACAAGAAACTACAAGAAATTTAGAGTGAATTGAGTTTGGTGCTTCAATTAGCTGATCATTAAAAGGCAAAGATGATGCTAGTGAAAAAAGTGTGAAACAATTTTTAGATAGCTATGCTAATAACACATCAGGGTACTCATCAGATATTAATCTCTTTAGTTATTTGGAGTATTTAATTAGGTAATTTGTTGTGATTGCTTGGTTGTACTTGGAAAAATTTTAGCCACTTGCTTTTCGCCTTGAACAGATGGCTTTTTATTTTTATTCAAATTAATTTCATCAAAAACTTTTTTGCAATTTTGTTGACACTTCCAAAAGCTCATTTCAATTTATTTAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAGGAGGATTTGACCATTAAAAAAGTAAAGGTTACAGAGAAAGCTTCTGTTGAAAGAATGATAGAAGTATTAAGTGATTCTGAAATTTTATCTTATTTATTAAGTAATAAATACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAGCAGTATAAAATACCAGCAGTTAATGCATGGCTGTTGGTATTTTTATTCTTTCACTAAACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGTGATACCAAGTTAGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTAATGATGGGACGAGTTCGGATTCTAATAACCAAGATACGAATCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTCGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTAGAGTTGCCCTACGTTAAAAACGAATCGGGAGCAGGGGACTCCACCACAACTAACAGTGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGCTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGTCCTTAAAAACAACCCCGGACCCAAAGTCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACCAAGGATGGCAAGGGGAAGGATGAGAGCTCTTGGAACAGTTCGGAAAAAACAACGGCGGAAAGTGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATACCTCAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGGGCGGGGGAAAACAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCCACAAATGAAGATCTACCGAATGTAATCACCCAGTTATACCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCGAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGAAGGGGTACTTGATGAGCCCACACCGAGTTGAACTGGGGCACCGACAAGCAGAAGGAGTTTGTGGAGAATCAGCTTGGTTTCAATGAAACCAGCGCCACCGACTCGCATAACTTTAAAAAAGCGCTGTTACATCAACCCGCCTACCTCATCAGTGGCCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTTCGAGCCGGCGCGGTGGGGTATGATATGACGACTGATACGAACGCTTCGACCTACAACCAAGCGCTCACCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAACGGCTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACGACTTATCCGGTCAAGTCCGACCAAAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATAAAAAAAGCCATCCACCTAAGGTGAAAGGCAGACAGCTCCAAAATATTTTTGACGATTGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGCCTGCTCCCACTAAATAAATTGAGAAAGATTTTCAAAGTAGTCAAAAAGTTTGAATATTTTGCAATTTGGGTTTGTAATCCCTTCTAACTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAAGACTCATAATGTTTAAATTAAAAATTAACAACTTCCAATTAGGTTTCAAACTCGTTCAGTGACCAGTGATGAACCACCTCCTTAAACATTTTTACGTTTTTCCAATCAATAATAAAGGAGGTCTTCACCATTAAACGCACAATTTCACTTACTTTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTACGACATGGAATTTAATGGCAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAACGAATATAAAGACTCAAACGACCAACGGCTCATAAAGATTGAAACTACTTTGGCTGCCCAAGGTGAGCAAATTAGTCAGCTAGTTCAAATTGTGTTCCTCCAAGGAAAACAAATTAAAGAACTTCAAGCGGAGCAAAAAGCACAAAGAGTTGAATTTAACGCGCGCATGGATCGCTTTGAAAGCCTTGTGCTTAAGAGTCTGGAATCAATCGGAAACACTTTGACTGATTTTGGTAAACGACTCGATTCAATGGAAACTCGTTTGGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTAGACAAAATCGATCCACCTAAATAGTTTTAAGCTAAAAATACCAGCAGCTAAACTGTTTTTGGTATTTTTATTCACAAAAAAGCTAAAACGATTTAACGACCAAAATTGGTTTTAAAAGTAATTTCAAAAAAAAAATAATAACACACTGAGTAGAACCATTTAAACATTCAACTTTTAATTAATTAAAAACAGTCAAAATTTGCTTTTAAAAATACTTTTACTTCAGTAACTCAAACTAGTAAATTTCAAGGGTTAAACATCTACAAACTTTGTTAATATTTCTCTAAATTTCGGGTTTCTAGTGTTTATGAAATTTAAGTACGGCGCTATTGTTTTTAGTTGTCTTTTGGGCGTTTCGGCCATTTTAGCTGCCTGTGGGGCAAGGGGCAAATTTAACCAAGTTGATGATGGCAAGATTAAGCTAGCTTCATCTTTAACTTCCAGAAGTGCGTCAACAGCTTTACAAAAAGTTGTTGAAAAGTACAACAAGGTTAAAGGCGTAAATGATTACCCCATTGAAATTACCCAAATTGCTGGCGGTTATGATGGCGGGCGGACTGATCTACAAACCCGAGTTAACGTTAAAGACACGACTAATTTTTATAACCTCATCTTGAACTATCCCGACTTAGTTTCCACTTTGGGACGTGTTGGGATGGAACTACCTTTTGATAAGGTTAAAACTGATAAGTTAGCTGACCGTTTTTTGGATTTCAACAACCGTATTAGTGCCATTTCCAAAAAGGGCATTTATGGCATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATTAATGGACCCGTTTTGCACTATATTTTGAACAGTGCACAAGGAAAGGTTAGTTCTGTCACTTCGGTGCAAAGAACAGCAGCTTCTGGTAGTGGAACAACAAATAATAATGGAGTAACAAAACCTTTGAAGATTGATAAAGAAAATGAATCTACAAAGAAACTTTGAGAAGAAATAGAAAACAAGGCAAAAGAAAACGGTAAGAAAACGAGTTCTTCAAGAAGAAAAAGAAATTTAAGTAGTTCTAAACAAATGAGCACTCAAACTCAACCTACTGACAATAGTAACGATGCTAATCAAAGCGACCAGGCGATAGAAAAGACTTGGGGTAAATATCAGGAGGTTGAAGGTGGTTTAAAAGATTACACCTTCAAAGCCAGTGTGTTTGAAAACTGACACGATTTGCTTGACTTTTCTAATAGGGTAGCTAAGTCATTTAGCAATATAAACACTAACACCAATAAAAAGGGCACCGATATTCAAGGTGTTCTGGGAATCGATAGCACTCCTAACTCCTTATTTACCTCTGTTTTTGCTGCAGGTGGCGGTAACTATGACAACTTCTTTTATAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCTTACCAAAACTTACAAAAAGTTTATGGTGATTACAAAGGCTTGATTGACAGCAATGGTCTCTTTGTTAATAAGGGTGGTTCTTATTCATCGAACTTCCAAAAGTTTCACCAATTAGCTTACAGTATTTCCTCGACTTCCGGCTTCTTCTACTCGTTTGCTGGTGAAAACTCAAAGCGCTTGAAGTTTGATGGCGGTAATTTTATTGAGTTTCCTGGATTTACTCAAGCAATTTATGCGCCTGAAAATAATAGTCAAAGTGGTGGAGAGAGTGGCGATCAAACCACAAATAATGAAGCCAATCTTTTAGGCACTTTCAAAATAAAAAGCTCTAATAAATCTAAATCTTCAAGTTCCAAATCAAGCACTAAAGCCGAAACAGGCAAAACTAGTGGTGGAGACCAAAACCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAAAGGCGAAGGCGCTCAAAATCAAGGCAAAAACGGAGGTGAAGAAACAACTATTTACCTTTATAAAAACTCAATTCCATCTGGAAAAAATAAAGGCGAAAATGCAATTCTTATAGATAACAAAACTATTGTGGAACAACTAGAAAGTGCGGCTAAAAAAGAAGATAAGAAGAGTGGCGAATCTACCACAGAACAGACACAAATACAAAGTAAAAGTGTAACAGAACAAAAACAACCCAAAATCATCGGTTACACTACCACTGGCAATGTTCACGAAGACAACAAACACATTTTCCCAATTGACAAACTTACATCTGATCGAAATTTTGACCGCAAAATTATTGTTGGTGCCACTGAAGAAACACTCGATAAATCGAATACTTTGCAGTCCAATGAAGCCATTGTTTTACCAGCACCCTCTAAGTACAAAAGTACTGACACTAACAAAGTAACCATCACCCAAGGTCCGAATATCATTGGAATTCACGCCAACGAAAAGGAAAATGCAGAAACACAAAAGTTTGTGGATTGATTCTTAAACAGTCCGCAAACTTGAGAGGGTAAGGGAAAAGGTAAAGAACAAACTAATAAAACCGCAGCTGAATTCTTTGCTGAATCGGCTTCTTATATCTTGCCACTCAAGGAAATTTTTGAGCAAAAGAATGAAAAGAAAGAAGGCAGCGATCAAAAAGATAGTAAAAGCAATGGTAGAGGTAAACAAACTAACCTCTACACCGAAAAGGCCTTGGAACTATTTAGAGGTATTAGTACCGATCAAATAGTAAGTTACAGTGACCCAAGTGACTTTAGGAGTGGTCGTTTCCGTGATGGAATAGGTGCCACTTTTAACGCAGCAGTAAGCTCTAAAGCATCTTTTGAAAAGTTTGTTCAAAACTTTATTGCAACTTTAGGTAGTGATATTTAGCTAATCACTTTTGCAGTCCAGTCAAGGGTTTCATAAAGCTAGCGCTTTATAATCAAATAATGCTTTAAAGCAATTTAGACGACTCCATGAACGACTGACAGTGACTCAAAAACCGGCTGGTTAACGCCAAAACTAAGGCTGTTAGTTTCTGGCTAGCCCAAACTACCAGCACCATCTTAGATATTGCTGAACTAATTAAATGTTGTTCGGATTTAAAGAATACTTCCATTAATGGGTTAGTTGATCTTATAAATCAACAAGAAAAACTCGAATTTAACTTAACAAGGTTAAAGGAAATTGATGGCGAGGATGCACGACAGTTGTTTGGCATTGAAGGTAATGTTTACAAACACTTCCAAACTGAACTTAGTCGCTTTTACAAGCAAACGCGCAAACACTTTCGCGAAACTGGTTCGGAAAGTCTCTTTTTAGGTTTACCAGTAATTGAGGGCATTAACGAGTTTAACGATGTCTTTCGTGCTCCTTTACTCTATGTGGGGGTTAAGTTAAAAGTAGCACCACGACTTGAACGCTTTTGATTGGAAATAAACCGCGAGGAAATCTTTTTAAATCCGACCATCATCGGGGTTGAAATTAACAAGCGTAACAGCTTGTTTAAAAATAACTATGACACCACTAAGGTTGATATTAACCAGGCATTGGAAATCTTTCGTGAGTTGGAATACCAATTTCGGATGCCACTCACCTCGGAGTTAAAAAGTTTTTCCAAAAAGGCTAAGAGTGACTTTAACACTGAAAAACGGACTAACTTCCTCACTAATAACGTTCTTTTAGGAATCTTTGACGTTAAGGGGGATCAACTGTTCCAAAACTTTAACGAAATTCTAAACACTGATCCTGATGTTTTGGATGAATTGTTAAAGGATCGCCGCGATCTCTTATACGACAACCGTGAGTTTCGTGAAAACTTTAACTTAAAGGGCACTTACCTCTTTAGCCACTTAGACATCTTCCAGCAGTACGCTGTCAAACAGGCATTTGATGGGGATGTCATTATTGAAGGTCCACCTGGTACTGGTAAGAGTGAAACGATTGTCAACATCTTGGTCAACCTCGCTTTAAACAAAAAGAAGGTGTTGTTTGTGTCAGAAAAGGTAACTGCCTTAGATGTCGTTTACAACCGGCTAGGTAGCTTTAAGCACATTGCCTTATTTAATGCTTCGGTAGCGAGTGAGAAGAAGCGCTTTTACAGCCAGTTCGCTGATTATGAAAGCTTCTTTACCGATAACTTTTCCAAGAAGGATTTAGTTAACGAAATGCCGGTGTTTGACGGGCAGTGGGTTGATAAGATCCTAAGTGAATTCACTAACCTTCAAAACATTTACGATACCCAAATTAATTCGGGTAACCAGAGCTATTCCTTCAAGGAAATCCTAAGTAGTTTTCCAATACTTGATGTCAGTTACATTAAGATCAAGGAACACGATCGTTTTGATGAATGAGTGCGGGTATTTTCCTCACAAGTTTGATTAGAAAAACACTTAACTTACTTAGCGTTTAAAGCGGAACTGAGCAAACGCTGGCAAAACATCGATAACTTCTACGCTTTAAAAGACTTACTGGAAAAAAGAAAGAACATCCGCGTGTTGTGCTATGTGTTGGATTACTTCGAGCAGAACAACAGCATAATCAAACCGAAGCGGGTATTGCTCTATACCCCCACTGAACGCGGGCAAAAGCAGTTGCACCAACTCCAGCAAGACGTTGCTAAGTACAACAGCTTGCAACGTTTTAAATCGGCTGCTAAGTTTGAAACAATTAAGCTCAATTTAGCTAACAAACTAGCACAAAACGCCAAGCCTTTTTTCTTTTCTTGATTCATTCAAACACACGCGCAAACACTGTTGGAAAATCTCGTACAAACCCAAAAGCAATTGGTTAAGGCTAAGCAAAGCTACTTAAGCAAAATTGAGCAGTATGTAGTTAGTTGTAAACGGATTCTCAAAGCTACCATTTTAGCCAACTTCTTTGAGTTGTACCAAACCAATAAGAATGAACTATTGGATATCTGTCGGGAAGCCAAAAACCCTGTTTTAAAGGAAATTACCTGGTGGTTTAAGAAGAACTTTGCACTGTTATCAAAGCTATTCCCCGTCCACATTATGACGTTTGAATCAGCCGCCTTATTAACCCCGAACCAGCGCCGTTTGTATGACTATGTGGTGATTGATGAAGCTAGCCAGGTTTATCTAGAACGTGCCATTCCAATCTTATACCGTGGCGCTAAGTACATTATTGCTGGAGACACCAAACAATTAAAACCATCCAATTTCTTCCAAGCCCGGGCTGAGTATGATGTGGATGAAGAGTTTGAAGATGGTAACGTTGAAGCTGCGGTGCACTCGACTTCGTTACTACACTTTTTGAAGAACCGTTCGCGCATTTTAACGCTGCTCAAATTCCACTACCGCAGTGATTCCGCTAACCTAATTGCCTTTACTAATAATCGCATTTATAACAACGAGCTCATCTTTATGAATAAAGCAACCGCTGACAAGCAGGTCTTTATTGTCCATGATGTCATCGATGGTATTTGACGGAACAACCGTAACCTTCAAGAAGCACGCGATGTCGTACAACGCTTAGAACAGTTGACCCAAACTGCGGAATATCAAAAATCATTAGGGGTGATCTGTTTCAATAAAAATCAGGCCGAGTTAATTGAGTACATGATTGACAAGCAGAACAATCCGCTACTCAATGAGTGACGTGACCGGGTGAACGCTCAGGGCGAGTATGTCGGTTTGTTTGTCAAAAACATTGAAAACGTTCAGGGTGATGAACGGGACATTATTATCTTCTCGTTGGGCTATGACCGTTCTGTCAACAGCTATGGCCCGATTTCCAAGCAAGGTGGGGAGAACCGTTTGAATGTAGCGATTACCCGTGCTAAGCAGCGGATTGAACTGTTCAAGACTAACCGTGCTAGTGATTACAATGGTTTGAGCTCCAATTCCTTGGGTAGTAAGTTATTGGTGGAATACTTACTGTACTGTGAGGCAATGGCCAACAACCAGGGTGAGAGCTTAGACTTCCAAGCAACCCAAAAACAAGCACCCAAGGCCAAGTACGAATTGGAATTGGAAAACCAGTTCTTTAATGAGTTGGAACTTATTTTTGGTGAGCAGTTTACGATTAAACGCAATGTCAATGAGGGTGCATACAGCTTTAGCTTTGTCTTTTACTTTAACGAAAACCCTTACCTAGCGGTTGACTTTAACCCTGCTTTACCCCACTCCAGAAAGGAAGTGAGTGAAAACATTATTTACCGCGAACAATTTTTGAAAAAGCGGAAATGGAACCTCGTTAACATTTGGTTAGACGAGTGAAAACTCAATCCAGGTGGTGTGTTGCAAAAGCTTAGAAATTGTCTAACTCACAGCGAAAATGAGTTTGAAGAAATATAATTAAACGATATATTTCTCGTCATTTAAGAGAAGTGTCGCTAGGCACTTCTTTTTTATTTGCCCACTTTATCACATTATGAACAATCAATCAAATCACTTTACCAATCCTTTGTTACAACTGATTAAGAACGTTGCCGAGACCAAGAATTTAGCCATCGATGATGTTGTTTTGTGTCTTAAAACAGCATTAGAACAAACATACAAGAAGCATTTGAACTATGTCAATGTGGAAGTCAACATTGACTTTAACAAGGGCTTAATGCAAATTGAACAGCTCTTCGATGTTGTAGATGATAACAATGAGGATTATGATGACTTTTTGGAAATGCCTTTAAGTGAGGCTAAGAAGCTCAATCCGAACCTAGAAGTTGGCGGGGTGTTGCGCAAACCGGTTTCACTCAAAGACATTAAGGGCGATCTCATTAGCAAAATGGTATTACTCTTCAACCAAAAGATTAACGAAACAGCTTTTAAAACGGTGATGAGTGACTTCATTAATGAAGTCGGTCAGGTAATTGAAGCTAGAGTAGAGGACATTGATACGAACAAAGATGGTGGATTGAAGGGTTACATTGTTAACTTGGAAACCACCAAGGGTTACATGCCTAAACGTGAGTTGAGTAAGGGCGAAAAGCTAGATATTGGCAAGAAGTACCTCTTTGTGATTAAGGAAATCCAAAAGCAATCTTCGATGTGACCAATTACGCTTTCCAGGAGTGATTCGCGTTTACTGGAATTCTTGCTAAATTCCAACACTCCCGAAATTGCTAACGGTACGATCGAAATTAAAAAGATGGAACGCTCTCCTGGTACCAAGTCTAAGGTGGCCGTGATTTCCAAAGACCCAGTAGTTGATCCGATTGCGGCCATTTTGGGTCCCAAGGGCGAGCGCATTCGCGGCATTAGTGAAGAGTTTAACGGTGAGATCATTGATATTGTCATCTGAAATGAGGACAAGTTAAAGTTCTTGGTCAACGCTGTTTTACCGGCTGAAGTGGTCGGTTACAACATCCTTCAAGACGATGAGCGCGATACCAGCATTGAAATCGTGGTACCCGCCAACCAGATTGCCAACGTCTTTGGTTTTAAAGGAATTAACATTCGCTTAATTAGTAACCTAACGGGTTGGAGTAGTGTGGACGTTTACACCGAAAAAGATGCCGCTGAACAAGGGATTGAATACACCCGGGTCAACTTTCAACCCCAAGGTATTTTTGGCATCAAAAAGCGCCGCGACAAGATCTCCAACAATCCACGCAACAACAACCAACAGCTCGCATCTGATAAGGTGTTTTACACTTCGAAGGCCAATGTGGTTGATGATGAGATCATAGTCGATCTAGCAAAGCAAGCGGAAGCCAAACGGGTCAAGCAAATCAAGCAGGAAGCAACTAAACCCGAGTTGCAATTACAACAAGAACTTAACCTCGAAGCAACCCCTAAGGTAGCTGCTCCAACCCCAACCCCTGCACCCCAACCAACTCCCGCACCAACTAAAGTTGAGCCAGTACCACCACCAGTAAGCGTTACACCTAAGCCAATCCCCAAGGTCAATAAGCCCAAGCCAGTGGTTAAACCAAAATCAGTCTTTTCGATTACGGTTGAAGCGGATGACAGTAAGACTAAACCAGAAAAGTCAAGCGCTAAAACTAACACCCCACAAACGAAACAAACCTTTGATAACTTTGACGATCTCTAGTCGTTAATCTAACCATTTCACACTATGCAAAGTTGGCGCCAATGTTTAGTTACCAGAAAGTCTTTTGCTAAGCCGCTACTTTTGCGTCTTGTAAAATTAGAGCATCAACTACGGGTGGATTTAGCGCAAACACTACCAGGTCGGGGGTATTATTTAAGTGTAGCTGCTTTAAAACTGGAGCGGAAAAACTTTAAAGCACTGTTACAAAAACGCTTAAAGGTAAGCTGTTCTGACGCAGAGCTAGATCACATTATTGCTTGTTTAAAGGAAAGGGAAAGCGATGTCAAAGCATAAACCACGTCATTTTCAAAAGAATAAGTTTGACAACCGGGCTAAAACGAGTGCCAAACAACAGTTTCGCCAGGTAAAAACCGGTGTTAAAGATGGTGTCTTTGTCTATAAGGGACCACTCACTGTTAGTGAGTTTTGTCTCAAAACCAATATTCCGACGGCCAACATCATCAAGCACTTCTTTTTAAATGGGGTTCCCTTAACCTTAAATTCGGTGTTATCGACCGAACAACTAGCTGACGCTTGTGTTAACTTTGGCTTTGACTTTAAGGTAGAAACCGAAATCACCCACGACAACATTATCTCCAACATTAAGTTTGACGATGATCCCACCCAGTTAAGTCCAAGACCCCCGATCGTTACCATTATGGGTCACGTGGATCACGGGAAAACATCCTTGTTAGACGCCATTCGCCAAACTAATACAGCAGCCAAAGAGTTTGGTGGCATTACCCAAAAGATTGGGGCGTACCAGGTCAAAAACCAGGAGGGCAAAACGATTACCTTCATTGATACCCCCGGTCATGAAGCCTTTACCGGCATGCGCGCCCGTGGTGCTCAAGTGACTGACATTGTTGTCTTAGTGGTAGCGGGTGATGATGGCTTAAAACAACAAACTGAGGAAGCGATTTCCCACGCCAAGAGTGCCAAAACCCCGATCATTGTCTTTATTAACAAGATGGATAAACCAACCGCTAACCCCGACATGGTGATCCAACAGCTCAACAAGTTTGATTTAGTCCCTGAAGAATGGGGTGGTGATACCATCTTTGTCAAGGGCAGTGCCTTAACCAAAGAGGGGATCCAGGAGCTCTTGGACAGTATTCTGTTAGTAGCGGAAGTGGAAGATTACAAGGCCAACTTTAACGCCCACTCATCGGGTTATGCGATTGAGGTGCAAACCACCAAGGGGTTGGGTCCCACCGCTACCATTATTGTTAAACGTGGTACCCTAAAGATTGGTGACATCGTTGTCTTGGGTCCCGCTTGGGGTAAGGTTCGTACCATGCAAGACGAAAACGGAGTCCATCTCCAAGAAGCTATGCCCTCTAAACCAGTACAAATTTCGGGGTTTGACATTGTCCCAGTTGCTGGGGAAAAATTCATTGTCTTTGACGATGAAAAGGATGCGAAGTTAATTGCCAATAAGTTCCGCGAACAGCAAAAACAAAAGCTCAATACCACCCAAATTAACGAGGTGCTCAAACAAAAGATTAAGAGCAAGGAAATTAAGGTTTTAAATCTGATTTTTAAGGTTGACAGTGATGGTAGTTTAGCGGCCATTAAACAAGCTATGCAGAGCATTGATGTGCCGGGGATGAGCGTCAACATTATCCACTCAGGTGTTGGTTTAATCTCGGAAAACGACATTATGTTAGCCAAGGCGTCCGGGGCATTACTGTTTAGCTTGAACCTCGGTTTAAGTCAAGTTGTTAAGAACATTGCTAGTCTTCAAGGAGTGAAGGTCGATGTCCACTACCACATCCCTAAGTTAGCGGAAGAGATCGAAAACATCTTAAAGGGCCAGTTAGAACCAGTGTACGAAGACGTGGAACTGGGGCGTGCTGAAGTGTTACAACTCTGGTACCACTCCAAGGTGGGGCACATTGCTGGTACCTTAGTTAAAACAGGTAAGGTTAAACGGGGCGCCTTGTGTAAGCTGTTACGCCGTAACGAAACCATCTATGAAGGCCGGGTGGACTCATTGAAGAGTGAAAAGAACCCGGTCAACCAGATGGAAGCGGGGAAGAACTGCGGTATTGTTATTAATGGGTGTGAGGACATTCAGGTGTGCGACATTATCTTGGTGTACGAAAAGCAGGAGGTCAAGTCCAAAAGTTAACGATGGCTAGTTACAAAAAGGAACGTCTGGAAAACGACATTATTCGTTTAATTAACCGCACTGTTATTCACGAAATCTACAACGAAACGGTCAAAACCGGTCATGTAACCCACGTGAAACTATCGGATGATTTACTCCATGTAACAGTTTACCTAGACTGTTATAACCGTGAGCAAATTGACCGCGTGGTGGGTGCCTTTAACCAGGCCAAAGGGGTGTTTAGCCGCGTGTTAGCACACAATTTGTACTTAGCCAAAGCCGTGCAAATCCATTTTGTCAAGGACAAAGCAATTGATAACGCAATGCGCATTGAGTCTATCATCAACTCCCTTAAAAAATCAAAGCCTAATTAAAATTAATACTGTTTAATGAAACCACCTAAAAAGCCAGTCAACCCACCGTTTGGTAAGCTAACTGATTCTAAAGCTGGAGTAGTCAAAGCTACCTCTAGTCAAGAGACCAAAAAAGTAGCTGACACCAAACCCAAAAATAAGGGTGGTTTGTTTAGCTTCTTTAAAAAAGACAAAACGGAAAAACCAGCTAAAGCAGCCAAACCCAAAGATGCCTTTAAGAGTGCGATAGCTGAATTGAATCCGAAAGCCAATCCTAAGACAGTCAAAGCGGATGTTGCACCGGCTAAAATTCCCCACAGCGATAAGGGTACGGTTACCCCTGTAGAGTTAAAACCCCAAACGGAAGCACCGTTACCACCAGGAGTTAAACAACCTGATCCTAAAAAAGATAAACCTAAGGGCGGTTTATTTGGCTTCTTCAAAAAAGATAAGAACAAAGATGTCAAAAAGGAGCCTGCTAAGCCAGCTACTCCAGTAAAAACTGAACCAACCTCACCTAAGGTAGAACCAACTAAGGTCAAACCACCGGGCGGTGTACCGACTAAGCCGGTAGTGGAAAAACCAGTGTCAGCTCAACCAACGGTACCATTACAACCAGAACCTACTTTCCCAGTCAAAGCAGCACCACTACCACCAGGTGTAAAAGCAGAACCGGAAAGCAAAAAACGCTTTGGTTTGTTTAAGGCATTCCAGAAAGATGACGCTAAACAACCCAAGCAAAAGCTCAATCTCCAAGACCAACAGGATCGCTTTATTGATGACCCCACCGCCAAAAAGCACTTTAGCGCCTTTAACCAAAAAGTTGGTAACTTACTGAAGGACAAAAAGACCCGTAACCGTGATTGGAAGATTGTGGGTTGAATCCACGGTTTAATCCTTCTGTTCTTCATTCCACTGTTAGCAATCATGAATAAATTTGTGACCCTCCCAGCACAAAGCTATCCTGCTGTATCACTACAAGTATCGATTAACAATGCGTTGTGGGGGATTGCGATCTTTGTGATAGCCAACATTGCTCTACCCTTCATTACGATGTTCGTGCTCTTCTTGACTGGGGTCAGAGATGTGCATGCTAGTCGGCCAGTGCACTATTTCATCTGGGTGTTAATGTTGTTAAACCTTACCTTCTTAGTAATTAGTTGTTGTTTACTGGCTGCTGCTTATGCTAACTTAGACCTTTACAACATCTGACGCAACCTTCAGGCTTTAGACCCGAATAATTAGGGCTTGAGTTCTATGCAGCAAACCTTGATCATCGGTGCTTTTGATGGTTTACACAAGGGTCATCAATTGTTAGCACAAGCGGCTAGTGGTCCAGTGGTGGCCTTGTTAATTGCTAACATTCCCAGTCTACAAAGTGACTGGCTATACGAACCGAAGCAACGGCAAGTGCAGTTACAACAACACTTTCAGTCCGTGGTGCACAGCTATGATGTGATTGAACACAACATTAGTGCCCAAGCGTTTTTCGATCAAATCATTAGCCCACTACGGTGTCAGCAATTAGTCGTGGGTGCGGACTTTTGCTTTGGTAAAGACAACCAGAATGCTGATTTTTTAAGACGTTTATTTCCCAATACCACGATTATTCCCAAAGACAGTCAAACGCTGTCCAGTTCTACCGTTCGCCAATGGTTAAAGCAGGGCCAGATAGAACAAGCCAACGCCGTGTTATTGGAACCCTACTTTAGGGAAGGGGTGGTTATTCGTGGTAACCAGCAAGCCCGGTTTTTGGGCTGACCAACCGCTAACATTACCTTAAAGCCGTACATGGTGCCGTTGCGCTGTGGGAGCTATGTGATTACGGTGACTTACCACCAAACCAATTACCCGGGTGTAGGGTTTATCAGTTACAAAAACGACCAGTTAGTCTGTGAAACCCACTTAATTGGTTTTAGTGGTGATCTTTACGGTAAACAACTACGCTTTACCTTCAACCAGTTTATTCGCCCCCAGCAAAAGTTTAGTGGGGTGCAAGCGTTGCAAAAAGCGATTAGTGGTGATTTAAAGCAAGCGCAGAAGTGGTTTGCTCAATCAACAAATTAAATAAAATTTAAACCTAATTTAACTTATGGAAAGTGCCCCCAGTGGTGGCCTGTTGGCCTTAATTATTATCAGTATTATCCTCCTGGCTTGTATTAGTGCCGTGGTTTCGGCTTACGAAACCGCCATTACCTCAATTACATCATACAAGTGGAGCAACTATGTCAAAACGCATAACAAGCAAAAGAAGTTAACGACCAAGATTGTTAACCACTTCCAAAAGAACTATTCGGCTTGTTTAATTACCATCTTAGTGGCCAATAACATTGTCGCCATTTTGGTTTCCAACATCCTCTTTTTGGCTTTAGATCAATCGATTAAGAATCCGGCCATTTCCAGTGCCTTGAACCTCTTAATTAGTGGGGTGTTATTGTTAATGCTGTGTGAAATTACTCCCAAAACCTTAGCACGGATTAACATCATCCGCGTCTTGGTCTACTTTGCGGTGGTAGTGTATTTTTTCTACATCCTTTTTTGACCAATTACCAAGTTAGCCAGCATTATCTTTGCCAAATACGAAAAGGCACCCCCAGTTTCCCGCCGTGATGTTTACTTCTTTATTGATGAAATTGAGCAAAACGGGTTATTTACCAAAGAAGACGGGCAGCTAATTAAACGGACCCTAATCTTTGACCAGGTCTTAGTTGACCAAATCATGATTAAGTGAAACCGGGTGGTGTACTGTTATGAAGGTGATCCAGTCAAGACCATTAAGGAAAAGTTCTTGCACGGCCAGTTTTCCAGAATGCCCGTACTCGACCAGACAAGCAATGAAGTGGTTGGTTTTATCCACCTCAAGGACTTGTTTAGTTCACTCGAAAAGAGCAACGAGCCGTTTGTTTTACAGGAGTTATTGTATCCAGCTGTTTTAGTAAGTAACACCACACCCATCAAGCAAGCTTTGCGCCAAATGCGGTTACACCGGGCCCACTTAGCGGTCGTGCAGGACAAACACCACCACACGATCGGGATTGTTTCTATGGAAGACATCATTGAGGAATTAGTCGGGGAAATTTACGATGAACATGATGAGGTGGAGGCAGTGCAAACGTTGGACAACAACACCTGGTTAGTGTTACCGAACGTAAAAGCCGCCTTTTTCTTTAACAAGTGGATTAAACGGGATTTAGTAAAATCAAAAAACATGACGATCCAACGTTACTTGGCATCGTTTGAAAATGATGGGTTAAACACCCAGAATAAACTAGAAACGCCGTGGTTTATTGCTGAAGCAATTGTCGACAGCGAGAACCCCGAGCAAATTCGGTACGAAATTCGCAAAAAGAGTGATGTTGTGGACTAGAGCCTTAATCTTAGAGTTAAAAACCAATAAGCAAAGCCGCTTGTTGTGGTTACTTGCAATTCCCTTACTAATATCGTTAACCTTACTAACGTACGGTTTAGTGTTGTTTAGTTCCAGTGGTCGAATTGATCATGGTGATCACTTCCACTTACGTGAGCGCTTTGTGTTAACGACCGAAGAGTTAGTTACCTTTGTGGTAGCGAGTGTCGTGTTTGCGCTAACTGTTGCCTTGTTTGGGCTGGGTTGCTGGAAGTTGCTCCAAGGTCCAAAAGTGGATAGAACAAACATAAAGCTAGCAAACAGCAATCCTGCTCCCCAAGCCGTGGTATTGCAAGCTGATTGTGATCACTTTCAGGTAGGGGATCACTGTGTGTTTAGCGCTGAAAAGCAACACTTTAAACAACAGTTCAAACAGGACTTTTTGGGTAAAAGTAAGTTTTCCTTTCGCAATGAACTGTACCGTTTTTGTCTCATTGGTGTGTTAATTTCGCTGAATTTAGCGCTCAGTATGGTGGAAATCCCCGGCATTGTTCTACCGTGGGGCAGTTCAATCCAGTTCCGCTTTTTTAACACCGCCATCTTGTTTATTGCGATTCGCTTTGTTGGGTTACTCTCCACATCGTTAATTGCGATTATTACTCCATGGATTCACCTCCTCCTTCACCCCGTTCATACCCCGATTAGTACCGTTTTTTACATGGGCAATGACTTGGTAGTTCTGTGAATATTCTATTTCTTTTACTACCACATCTTTAAGGCCGAAGTCAAACAAACCACTACGGTTGTCAATAACAAGGAGTTTAGTCAATTAGTCAACACCCACAAAACGAAGGTAGCCAAGGCCTTGGCGTTAATTCCAGTTAATTTAATCTGTGGTTTTATTGAGGGTTTGGGTTTCTATGTCGGTTACTTCTTGATTTTGGGTAAATTTGGTAGTGTAGGGCATAAGATTTTCTATGACAGTCAAGCTAACCGTGACCTAATTAACAGTGCTAACGTGATCTATTTCTTACTCACTACTACCACCATTTTTAGCTTAAAGTACCTGTTTGAACTACTGTTCTTTTACAGTGTCGAGAAGGGTATACTTAACATTTCGCGCCACTTTGGTTTGTATTAAAAGGTAAATAATTTAGCTTTTTGTTTAATCAGCAATTTCCTTTATATATAAAAAAGGTAATACAACGATAGTATAGGGGTTCTAGGGGAGTTAATTTGCAGAAAACAGCTAAACCATTGTTTCACCTGGCAAACAATTTAAAGTTAATGCCATATGAGTGACGTCAAATACTTACAGGTCGAAATTGTCGATAAAAACACCATTCGCCTCCAGGAAGATGGCAAAAAGGGTCAACAAATTAGGTTAGATCAAATTATTAAGGTTGATCAAAGCAACATCTTAAACACTTTGGAGCAGGCACAACGCGAGGCATACGAAAGGGAGGCGCAAAGCCGTTACCAAACTAAGTTGGCCAAAGAACTGAGTGAAAAAGACAACACTTTTTTAAAGCAAGAAGCAGCTTGAAACCAAGCGCACAACACGCAAATCCAACAGTTATACCAACAAATTACTAACTTAGAAAATCAGGTTAACAACATCAAAAGGGAAACTGAAAGTAAAAAGGATAACGAATATCAACAACAAATAGTTAAGTTAGAAACGCAGCTCCAAAGCATTAAAAAGGAGACCGAAAGTCAAAAAGACTTGGAATACGAACGTAAAGCAAATAAAACTAAGGAAGAAAATCAACAAGAATTAGAAAGACAACGCCAGTACTTTTTAGAACAGTTAGAACAAGCCCAAAAGGACATTGAAGAGCTCAAAACGCGTGAAGAGCGTTTTAGTAAGTGAGCAATTGCCAAAAAAGGGAAGGAATTGGAAAAGTGGTGTTGGGAAGCTTACAAAAATTACGAAGAGTTGTTCCAAAACTGTGTGTTTGCCCCTTACAGTGAGCTAGTTAAGGGTGCCAAAAAGAGCATAGGTGTAGAAGACGATGAGAGCAATATTAACGAAAAGGCTGACTTTATCTTCCAGGTATTTAATCCCAATAATGACAAAGAACCCTTCTTTTCAATTTGCTGTGAAATGAAAACAGAGTTTACTGAAAGCAAGAGTCGCACTAAAAACGAAGATCATGTCAAAAAACTGATTGCCGACGCTAAGCGCGCTAAGTGCCAATACGGTTTTTTAGTAAGTGAATTGGAACTCAACACTGAAAACGATGTCCAAGTGCAACGGATGCATACCTCGAACAGTGAAGTGGAGGTTTACCTAGTGCGCCCAATGTTCTTCATTGTGATGTTGCGGTTGTTTTACTTTTTGGCCAAAAAGATGTTTGCCCAAGTTGATGTAAACAGCGAGTACTTAGATAAAGAAGCTTTAAATGCTAGCTTTAGCGATTTAAAGAAAAGCTTACTTGAAAAAACCTTTACTGATCTCAACAAGGTCTTTCAAAACAACATTGATGAACTAGAAAAGATTGAAGGATTAGTAACAAAGTTGAAAGCCGCTAATGAAAAGGCCTTAAATAGTCGTTTAAATAACTGGGAAGAAAAGATCCGTAAGTTTGAATTTAAGCTCAATAAAGACATTGTTAAAAAGCTTGAATAAGGTTCTTTTAAGTAGTCTTTTTAGGGTAGAAATTAGTTTCCAAAAACGGTTTTAAATTAGAGGCTTATTTTTAAAAATTCAAAATAAGCAAATATAATTAAACCGTCTTTTATCTGAATATATGAAACTAAATCTGCGATTTCCTAGCTATTTTTTACCCGTCGTTGCCGCTAGTGCTTTCCTTGTTAGTTGCGCCACACCTAACACTTACGAAGTACAACGCGCTGCCTTGATTCAACTAGTTGAAGAAGACGAACGACAAAACTACATTCAAAAGGGTAGTATGGGTGCAAATGCTGTTATGACTGCTGCAAAAGCAGAAACCAAAACAGCAGAAAAAACAGCAACTTCTACCAAAGCTGCTTCAATAGAACTTAAAAAAACTGATACTGATATTAAGACTACAACTACTACCGAAAACAAATCAGCATCCGGATACAAACTAGATACCCTCTTTGGTGACTACATCTTGTGAGTAGTTGACCACCTTTCTGGTTTACTTTTCTCACCTAAGACTAATAACAGTACAACTACTCAAAAAATCCAACTCATTACCGAAGATAAGGTGATTTTAGACGGTGGTAACTTGACCGTGGAAAAAAATCATGAACATGGCCATACTCATAAAAATGGTGAAACTCACGAACATGATCATGATCACCACGAAGGTGAAGAGGAAGTAATCGTTGGTCGTGCCCTTTCTTTTGCTAATGGTCTCTTCTTAGTTGTGGATCTTAAGGAAGAAAAACACGAAGAGAAGAAGGAAGCAAAATCTGAAATGTCAATGAACAGTAAAGATATGGTGATGATGACCAAAACTGAAATGATGTCTAAGGAAATGAAATCCGAACAAAAAATGGAGAAGAAGGAGGAACACGAACATCATCACAAAAAGCTGAGCCTCAGTACCACGGCTTACAAGTTTGGTCAAAGCTTTAACATCCTGGAATTTACTGGTGCTATGCACCACAAAACGGCACACAATAACGAAACCGAATTTAAAAATTTAGGCAAAAAATACGGTGGTATGACCGAGTACACGATTGTCGACTTTGACTTTAATCCACCAAAACCAACTAAGTAAACGGCTTAACTAGCGCAAACAAGTCCCAATGCTTTTAGCTTGGAGCTTTTTTGCTGTTAGTAAGCTTCTTTTAACAATTAAATGGGTCTTTTAAAGTGACACAATAAGATCGCCAAAAAGCGGGGTTACTACTTTTTTGAAGTTGTTTAATTTCAATGATTATGGGAAAATAATAAGGCCTGCAAGCGGCAAACTAGTTTGGAAAAAGATAAGGAATTAGTCAGTTCAATTGTGACTGCTAAAAGCATGATTGACCGGTTTTTCTGGTCTATTTTGAGCTTTCTGCTCACTAACTTAGTCTTTTTATTTGTGGCTTTTTTAATTCTGATTATTTACTTAATTTCCGAAATCACCCAACAGTTTGCCTTTGCTTTTATCGCAACGATTGTCTTCATCATTTTTTACAACATCCTCTTTTTGAGCTACCTGTTGACGATGTACATTAAAGGCTTAAAACAGATTGAGCAAAAGTCACGTTATTTACTCTTAGTGCTTGATGTTAAGGCTGATGAACTGCTTCCTTTCTCCTTTTTAGGTAGTTTAAGAAAGAGTCACATGCTTGAAGAAATGTTGTTAGAGCAGTAATTATTTGCTACAATCTTAAGGCTTTGGTCGAAGTGGATACACCGACAAGCGTAGTCATTTTGACCATCAAATTTCACCCTTAAGAACTGAGATATGAACGCCGCTAACGCAGTTAAATACCCTGAGCTCAAGATTAAGCTCGAGTCCTATGACAGCACCCTTTTAGACCTCACTACGAAAAAGATAGTGGAGGTAGTAAAGGGTGTCGATGTCAAAATTAAAGGTCCATTGCCTCTACCTACCAAAAAAGAGGTAATTACGATCATCCGTTCACCCCATGTGGATAAAGCATCACGCGAACAGTTTGAAAAAAACCGCCACAAACGTTTAATGATCTTGGTGGATGTTAACCAAGGTGCGATTGACAGCTTGAAGCGGATCAAGATCCCCGTTGGAGTAACTTTAAGATTTTCAAAGTAGTTAAAGAGAGAGTATGGAAATTCGTGGTATTTTTGGGGTTAAAGTAGGCATGAGCCAGGTGTTTACAACCAATAATGAGCGCCTACCTATTACCGTTATTTATTGTGAACCTAACCAGGTAGCAGGTGTTAAGAATGAGGCTAAGGACAAGTACAGTGCTACCTTGTTGAGTTTTGACACCGTTGAAAACAAAAAGCTCAACAAGCCTCAACAAGGCTTCTTTGAAAAAAACAACTTAAAGCCAACCAAGCACTTACAGGAAATCCGCAATATGACCGGGTTTGAAATGGGGCAACAAATCACACCGCAAAATCTCTTCCAAGTGGGTGAGTATGTTGATGTAAGTGCCATTAGTAAAGGTCGTGGGTTTACTGGTGCCATTAAGCGCTGGAACTTTAAGATCGGTCCGTTAGGTCATGGGGCTGGTTATCCCCACCGCTTCCAAGGTTCAGTCCAAGCTGGTCGTGGGGGTGCGAGTGCACAAAGAGTGTTTAAGGGCAAAAAGATGTCCGGACACTATGGTCATGAAAAAGTAACGGTACAAAACTTACGCATTGTTGGTTTTGATGAAGCTAACATGTTGGTATTAGTATCAGGGGCGATCGCAGGTCCTGAAGGTGGTGTGGTCTTCATCCGTACCGCTAAGAAAAAACCGGGGGTAGTGAAACCAATTGAGTTAGCTGTTCAAACCGAAAAGGCACCGGAAGCAAAACCGGCCAAGCTTTCCAAAAAGAAACAAGCCAAGGAGTTAGCGAAAGCACAAGCAGCTAACCAGCAAACTGTTGAAGCTAAGGTAGACACTCCAGTAGTAGAACCAAAACCAACTGAAGTTAAAAAAGCAGCACCAGTTGTAGAAAAGAAAGGGAAAGATAAATAATGGCTAAGCTTAAACTAATTAAAATCGACGGTTCCTTTGAAACTGAACCAGTGAAACTAAGCCCTGGTTTAATTGCTAAGGAACTCAAACAACAACCGGTGTTTGATGCCGTGTTAGTAGAACAAGCTTCCTGACGCCAAGGCACCCATTCCATTTTAACAAAGGGCGAAGTCCGTGGTGGTGGTAAGAAGCCTTACAAACAAAAACACACCGGGAAAGCGCGTCAAGGTTCAACCCGTAACCCCCACTTTGTTGGTGGTGGCATTGTGTTTGGTCCCAAGCCCAATCGTAACTACAGCTTAAAACTCAACAAGAAAGCGCACACGGCAGCCTTGCACACTGTTTGGAGTGAAAAGTTAGCTTCAGACAACACCCACCTAGTGGACCAAAACCTCTTTAACAAAACTGAGGGTAAAACCAAGGTGATGGTGCAATTTTTAAAGAGTGCCAAGCTCTTGGACAAAAACGTGTTGTTTGTAGTTAATACCCTTAACACCAACTTAGAACAAAGCACCAGCAACATTAAAAACGTGCAGGTCAAACACCTAGACAAGGTTTCTGTAAGGGATCTGATGTTAGCTAACGCTCTTTTAGTTGAAAAAGAAGTTTTAAAAGCTTTGGAGGGCAAATTTAAATAACCATGGATGTAACGAACGTACTGCTAAAACCAGTTCTAACCGAAAAGGTGTACTTCAACCAAATGGGTGAAACCAAGAAGTACGTCTTTGTGGTGAACCCTAAAGCAAGTAAAACACGCGTTAAACTAGCTTTTGAATTAGTGTATGGCATTAAGCCACTGAAGGTTAACACCTTAATTAGAAAACCAACCACCATTCGCGGTGGGAGCCGTTTTCCTGGTTTGAGCAAACTAGAAAAGTTGGCTGTGATTACCTTACCAAAGGGTATTGCCATTTCAGTTACTGGTGAAGCGCCAGAAAAAACCGATAAGCCAGCGGACAAAACCACCCTTAAGGAAAGTACTGTTAAGGAGATTAAGGACACCAAAAACAGTCCAGAAGCTGTTGTGAAAACTGCTGTGGAAGCGTTGCAAATTAAACCAACTGCCGCTCCTGTAACCACCGCCCCTCTACAAACCGTGGCGGTGAAGGTAGCTAAAGAAGTTAAAGAAGTTAAGGTAGAAAAGCCGGTTAAAGTGGAAAAACCAACCAAACCGGCAAAGGTAGCAAAAGAAGCTAAAACGACCAAAGTAGCTAAGGAAACTAAAGCTGAAAAGTCAGTTCAAACAACCAAAGTAGCTAAGGAAACTAAAACTGAAAAGTCAGCTAAAACTACCAAAACTACAGCTACAAAAACTACTAAAACTAAAACCACCAAAAAGGAGGTTAAAAAGTAATGCCTATTAAGAAAATTATTAGTCGTTCTAACAGTGGGATCCACCACTCCACGGTAATTGACTATAAGAAGTTATTAACCACCAATAAGAACAAACCAGAAAAGTCGTTGTTAGTTACCCTTAAAAAACACGGGGGTCGCAACAACCAAGGGAAAATTACGGTGCGCCACCAGGGTGGACGCAACAAGCGCAAGTACCGCATTATTGACTTTAAACGAACGCACTATGACAACATCGAAGCGACCGTTAAGTCGATCGAGTACGATCCGAACCGTTCTTGCTTTGTGTCGTTAATTACTTACGCTAACGGTGCCAAGAGTTACATCATTAGCCCTGATGGCATTAAGGTAGGTGATAAGATCTTGGCTTCCGAACACCCGATTGACATTAAACCGGGCTTTAGTATGCCCTTAGCCTTTATCCCTGAAGGTACCCAAGTGCATAACATTGAACTCCACCCTAAGGGAGGTGGTCAAATTGCCAGGAGTGCGGGGAGTTATGCCCGCATTTTGGGTCAAGACGAAACTGGTAAGTATGTCATTTTGCAACTTTTAAGTGGTGAAACACGCAAGTTCTTAAAGGAGTGCCGTGCTACTGTGGGAGTAGTTTCTAACTTAGACCACAACCTCGTAGTCATTGGTAAGGCTGGTCGCAACCGTCACCGCGGCATCCGTCCAACCGTTCGTGGTTCGGCCATGAACCCCAATGACCACCCCCACGGTGGTGGTGAAGGTCGTAGTCCCGTGGGTCGGGATGCACCCCGGACTCCATGGGGTAAGCGCCACATGGGTGTGAAGACCCGCAACATGAAGAAAGCATCCACTAACTTAATTATTCGTAACCGGAAAGGAGAACAATACTAATGTCACGCAGTGCTAAAAAAGGAGCCTTTGTTGATGCTCACCTCTTAAAGAAAGTGATCGATATGAACAAACAAGAGAAAAAACGTCCGATCAAAACCTGGTCCCGTCGTAGTACCATCTTCCCCGAATTTGTCGGTAATACCTTTGCTGTACATAACGGCAAGACCTTCATTAATGTTTATGTAACTGATGACATGGTGGGGCACAAACTCGGTGAGTTCTCCCCGACCCGTAACTTCAAACAACACACCGCTAACCGTTAGTATGATCGCCTTTGCAAAACAATTCCGTGTGAGAATCTCACCCCAAAAAGCCCGCTTAGTGTGTCAACTCATTGTGGGTAAGAAAACCGCTGATGCCCAAAACATCCTTTCCAATACACCCAAAAAAGCGGCTACCTTAATCGCTAAACTACTCAACAGTGCGATTGCCAACGCTACCAATAACCACGGCATGAATGGGGATGCCCTTTATGTCTTTGAGTGTGTGGCTAACCAAGGTCCGAGTATGAAGCGCACTATTCCCCGCGCTAAGGGCTCGAGTAACATGATTACCAAGCGCTCTTCTAATTTAGTGGTGAAACTATCGGATAACCCGAATGAACGTCAAGAATTAATTAAACAACAAAAGGCCCTAGTTAAAAAACGGGTCGAAGGTCAACAAAAAGCAAAGATGGCACGTCAAAAAGCAGTAACACCAGTGGTAAAAGCGCCAAGTAAAACTCAAGGAGGTGTACAAAAGTAATATGGGACAAAAAGTAAACTCCAACGGCTTGCGCTTTGGTATTAACAAAAACTGGATTTCGCGTTGAACCGCTAACTCCCACGCCCAGACTGCCAAGTGGTTAATTGAAGACGAAAAAATTCGTAACCTCTTCTTTGTCAACTACCGCAATGCCCAAGTTTCGAACGTGGAGATTGAACGTACTCAAGCAACCGTGGACGTATTTGTGTACGCCGCCCAACCCGCTTTTCTCATTGGTAGTGAAAACAAGAACATCCAAAAGATCACCAAGCAAATTAAGCAAATTATTGGTCGTACCACCAATTTAGACCTCACCATTAACGAAATTGGCTCCCCTATGTTATCAGCGCGCATTATTGCGCGGGATCTAGCCAACGCGATTGAAGCACGGGTACCGTTACGAACGGCAATGCGCCAATCCTTAATTAAGGTGTTGAAGGCTGGCGCTAACGGGATTAAGGTGTTGGTATCGGGTCGGTTAAACGGTGCCGAAATTGCGCGCGACAAGATGTACATCGAGGGCAATATGCCACTGTCAACCCTGCGTGCTGACATTGACTATGCCTTGGAAAAAGCGCAAACTACGTACGGTGTCATTGGTGTTAAGGTATGGATTAATCGCGGCATGATTTACACCAAGGGCTTAAACCGCACCCCGGCCCACATTCTCCACCCACAAAAGAAACAACCAAACCGCCAAAACCAACAACCACGCCACTTTAACCAGGGCCAGGTGTTGAGTGCTAATAAGTTAACTGGTAGTGATGTGGAGACCAGCAGTATCCAAGCACTTACTAAGCCTAACAAGGAGGACAAACAATAATGTTACAACCAAAAAGAACAAAGTACCGCAAACCTCACAACGTGAGTTACGAGGGCAAGGCTAAGGGCAACAGCTATGTTGCTTTTGGTGAATATGGTCTAGTAGCTACTAAGGGTAACTGGATCGATGCCCGTGCCATAGAATCAGCCCGGATTGCCATTAGTAAGTGTTTGGGTAAAACCGGTAAGATGTGGATCCGCATCTTCCCCCACATGTCCAAGACTAAAAAACCGTTAGAAGTACGGATGGGTTCTGGTAAGGGTAACCCGGAATTCTGGGTAGCAGTAGTAAAACAGGGCACGGTGATGTTTGAGGTAGCTAACATCCCTGAAAGCCAAATGATTAAGGCTTTAACCCGTGCTGGTCACAAGTTACCCGTGACCTGAAAGATTCTCAAACGCGAGGAGGTAAGTGCATAATGACCGTTGCAAAAGAATTACGCCAAAAGAGTTCCGAAGAGTTGGTAAAACTCGTGATTAAGCTCAAAGGGGAACTGTTAGAGTACCGCTTTAAGTTAGCACACGGGGAGCTAGACAAACCGCACTTAATTAACCAAACCAGGCGGTTATTGGCTACCATTTTAACGATTCTTACCGAGCGTAAGTTGAACTGACAAGAAGAACAAGCTAAGTACAAACTGCTCACTAAAAAAACGAACGAAGCAGCTGTGAATGCTTGGAAGCAACACCTAGAAGCTAACAAAGCGAAGCTGTTAAAGTCACGTGCTAAACGCGAGGATGCTAGCAAGAAATAATGAAACGTAATCAAAGAAAGGTCCTAATTGGGATTGTTAAAAGTACTAAAAACGCCAAGACCGCTACGGTTCAAGTAGAATCACGGTTTAAGCACCCTTTGTACCATAAGTCCGTAGTGCGAAACAAGAAGTACCAAGCCCACAATGAGGGTGAAGTACTCGCTAAGGACGGGGACAAGGTACAAATTGTGGAAACGCGACCGTTGTCTGCAACCAAGCGCTTCCGCATTGCCAAAATTATTGAGAGGGCTAAATAGACTATGGTAAGTTTCATGACAAGACTAAATGTTGCCGATAACACGGGCGCCAAGCAAGTGGGCATCATTAAGGTATTAGGTTCCACCCGGAAACGTTATGCCTTTTTGGGTGATGTGGTAGTGGTATCAGTTAAGGATGCTATCCCTAGCGGTATGGTCAAAAAGGGTCAAGTGCTACGGGCAGTAATTGTCCGCACTAAAAAGGGCCAACAACGTAAGGATGGTACCCACCTTAAGTTTGATGACAACGCTTGTGTGTTAATTAAGGAAGACAAGTCACCAAGAGGCACACGGATCTTTGGTCCGGTAGCGCGTGAACTACGGGAACGTGGTTACAACAAAATTTTGAGTTTAGCAGTGGAGGTAGTGTAATGCAACGAATTAAAAAAGGTGATAAAGTAGTCGTAATTACCGGTAAGAATAAGGGTGGTAGTGGCATTGTTTTGAAAATTATGCCCGCGCGCCAACAAGCAATTGTAGAGGGACTCAACAAAGTAACACGCCACAAAAAGAAGGACCAAACCACAAAGCGCGCTGCTAAACAATCGACCGGTAAAGTGCAACAAGAAGCGCCAATCTTCTTGAGCAAGTTAGCTTTATTTGACCAAAAGGCAAAACAACAAACAATTGGCAAAATTAAGTACGTTATGGACCCTAAGACTAATAAGAAGACCCGGGTCTTTAAGAAATCCAACAACACGCTCTAGTTATGAATAATCTCAAAGCACATTACCAAAAAACAATTGCCAAAGAGCTGCAAAAGAGCTTTGCGTTTTCCTCGATTATGCAAGTACCGCGCTTGGAAAAAATTGTGATTAACATGGGGGTGGGTGACGCCATTCGCGACAGTAAGTTCTTGGAATCAGCCTTAAACGAACTCCACCTCATTAGCGGTCAAAAACCAGTTGCTACTAAGGCTAAAAACGCCATTTCCACCTATAAGCTGCGCGCTGGTCAACTAATCGGTTGTAAGGTTACCTTGCGCGGTGAGCGGATGTGGGCCTTTTTGGAAAAACTGATTTATGTTGCCCTACCTCGGGTGCGGGATTTCCGCGGCCTTTCTTTAAAGTCTTTTGATGGTCGGGGTAACTATACCATTGGAATTAAAGAACAAATTATTTTCCCGGAAATTGTCTATGACGACATTAAACGGATCCGCGGGTTTGACGTTACTCTGGTAACTTCAACCAATAAGGACAGCGAAGCATTAGCTTTACTTAGAGCGTTAAACCTCCCACTTGTAAAAGGATAATATGGCCAAAAAATCATTAAAAGTAAAACAAACTAGAATCCCCAAATTTGCCGTACGGGCTTACACCCGCTGTCAGCGTTGTGGTCGAGCCCGGGCAGTTTTAAGCCACTTTGGTGTGTGCCGTTTGTGCTTCCGCGAACTAGCATACGCTGGCGCAATTCCAGGAGTTAAAAAAGCATCATGATAACAACTACAAAACCAATTAAAGCCCACTTTGATCCAGTGGCTGACTTACTAACGAAGATTAACAATGCGCGCAAAGCGAAGTTAATGACGGTAACCACAATTGCTTCCAAGCTCAAGATTGCCATCTTGGAAATTTTGGTCAAGGAAGGTTATTTAGCTAACTTCCAGGTCTTGGAAAACAAGAGCAAGACCAAGCGGATTGTCACCTTTAACTTAAAATACACGCAGCGCCGGATTCCCTCCATTAACGGGGTGAAACAAATTTCCAAGCCGGGTCTGCGGATCTACCGGCCCTTTGAAAAGCTCCCGTTAGTTTTAAACGGTCTTGGGATTGCCATTATTTCCACTAGTGATGGCGTGATGACTGATAAGGTAGCGCGCTTAAAGAAAATTGGAGGGGAGATTCTCGCTTACGTTTGGTAAACAGTTATGTCAAAAATAGGAAACAGAACGATTACACTAGACCCCGCTAAGGTCAACCTTAACTTCCAGAAGGACCACATTGCCGTTAAGGGTCCTTTGGGTCAAATTGAGCTCAAACTACCCCCTAACTTACCGCTCAAGTTTGAACTGAAGGATAATAACCTCCAAATTACGCGGAACAACGAGTTAAAGCAATCCAAGATCTTCCATGGTACTTACAACGCGTTAATTACCAACGCCATTATTGGGGTTACTCAAGGCTTTGAAAAGAAGCTGCGCTTAGTCGGTGTGGGTTACCGTGCTAACGTGGAAGGGGAAACGCTCAACCTCCAGTTGGGTTACTCCCATCCCATTAAGGAAAAGATTCCCAAAGGCTTAACAGTTAAAGTAGAAAAGAACACCGAGATTACCATTTCGGGAATTAGTAAGGAGTTAGTGGGTCAGTTTGCCACTGAGGTGCGCAAGTGACGCAAACCCGAACCGTATAAGGGTAAGGGAGTGTTGTACTTTGATGAAGTGATTGTCCGTAAAGCTGGTAAAACCGCGGAGGGTAAAAAATAGATGAAAACACGAACAGAACAACGGCGCTTACGCCATAAAAGGATTGTGAAGAAGATCCGCGCCACTAACCACGACAACCGCGTTGTGTTAATGGTGATTAAGTCCTTAAACCATATTAGTGTGCAAGCGTGGGACTTTTCCCAGAACATTGTTTTAGCGTCCTCTTCCTCACTTGCTTTAAAATTAAAGAATGGTAACAAGGACAATGCCAAGTTAGTGGGGCAAGACATTGCTGATAAACTAGTGAAGCTCAAACTAACCAACGTTGTCTTTGACACTGGTGGTAGTAAGTACCACGGCCGGATTGCGGCATTGGCCGAAGCAGCCCGCGAAAGGGGGTTAAACTTCTAATTTATGACTGATCAAAACCAAAAAGCAAATCAAGGTAACGGCTTGCAAACCACTAACCTCCAAGCCCACGCACAGCGTAAACACAACCTCCGTCCTTCCTCTGAAGGGATTAAGAAAGCCGTGTCCAAAAAGGAAGGTGGTGGTCACAACCGTAACAACCAAAACCGTCGCTTCCAAAAACCGGCTTTCAAGAGTGAGTTTGAAGAACGCATCGTCAAACTAAAGCGCATTTCTAAAACCACTAAGGGTGGACGGAACATGCGGTTTAGTGTGCTGGTCGTAGTTGGTAACCGCAAGGGCAAGATCGGTTATGGGATTGCTAAGGCCTTGGAAGTACCGAATGCGATTAAGAAAGCCATTAAAGCAGCGCACAACTCACTCCACACAATTGAAATCCACAAGGGTTCAATTTACCACGAAGTAATTGGCCGTAGTGGGGCGAGTCGCGTGTTGCTCAAACCCGCTCCTCAAGGTACGGGAATTATTGCTGGTGGTGCCATTCGGGCCATTATTGAGCTCGCTGGTTACAGTGACATTTACACCAAGAACCTCGGTCGCAACACGCCAATTAACATGATCCATGCCACCATGGATGGGATCTTAAAGCAGCTCTCTCCACGTCGGGTAGCGATCTTACGTAACAAAAACCTCAACGAACTTTAGACAATGGAACTCAATCAACTCAAAAGTGTCCCCAAAGCGCGAAACCACAAGACCAAAACTTTAGGTCGTGGGCACGGCTCGGGTTTAGGTAAAACATCTGGTCGAGGCCAGAAGGGGCAAAAAGCGCGCAAGTCCGGTTTAACCCGTCCTGGTTTTGAAGGAGGACAAACGCCACTGTACCGTCGTTTACCGAAGTTTGGTAACGCACGCAAGGGCTTTTTAAAGCAGGAGTGGGTGGTGTTAAACCTCAATAAAATTGCTAAATTAAAGCTCGACAAAATTAACCGCGCTAGCTTAATTGAAAAGCAAGTAATTAGCGCAAAGAGCCAATTACCAATTAAACTAATTGGCAACACTAAGCTCGAAAAGCCGTTGCACTTTGAGGTGCACAAGGTTTCCAAGCAAGCTTTGAAAGCGGTTGAAAACGCCAACGGTAGTGTGAAGCTGTTAGAGAAATAATGCAAGCCAAACCAACAACTGCAGTTAAACAAAAACAGAATTTTGGGCAACGTTTGTTCACTTTGTTGCGCAACCGTGACTTCATGATCTCGTTTTTAATAACGGTCGTGCTGTTGGTTTTGTTTCGTGTTTTAGCGATTATTCCGCTCCCCGGCATCCAAGTCAACCAAACCGGGCTAGACCAAAACTCAAACGATTTCTTCTCACTGTTTAACCTACTTGGTGGGGGTGGATTGAACCAACTATCGCTGTTTGCGGTGGGAATTAGTCCGTATATTTCGGCGCAAATTATTATGCAGCTGTTGTCAACGGACTTAATTCCCCCGTTATCTAAACTGGTGAACAGTGGGGAAGTGGGTCGGCGTAAAATCGAGATGATTACGCGGATTATTACGCTACCGTTTGCGTTGGTGCAATCGTTTGCTGTGATCCAAATTGCCACTAATTCCGGTGGCGGTTCTTCACCTATTACCTTAAAAAACAACGGTTCGGATTTTGTCGCGTTTTACATCATTGCCATGACTGCTGGCACCTACCTGTCGGTCTTTTTAGGTGACACCATTTCCAAAAAGGGAATTGGTAACGGGATCACCCTGTTAATTCTTTCGGGGATTTTAGCCCAGCTACCCGAAGGATTTATAGCTGCTTACAGCGTGTTATCAGGTGTAGTGGTAACCATTAACGCAACCTTAACCACTGCCATTAGCTTCTTCATTTACTTCATGGCGTTTTTGGCATTACTGTTTGCCACTACCTTTATTACCCAAGCGACCCGGAAGATTCCGATTCAACAATCCGGTCAGGGTTTAGTAACCGAATCCTCTGCGTTACCTTACCTCCCAATTAAGGTCAACTCCGCCGGGGTAATTCCGGTGATCTTTGCGTCTTCGATTATGTCAATTCCGGTGACGATTGCCCAGTTCCAACCGCAAACCGAATCACGCTGGTTTGTGGAGGACTATCTTTCACTGTCCAAACCAACTGGCATTGTCCTGTATGGTATCTTGGTGATTCTCTTTAGCTTCTTTTACAGCTACATCCAGATCAATCCAGAACGGTTAGCCAAAAACTTCGAGAAGTCCGGGCGTTTTATCCCGGGTATTCGTCCCGGTAAGGATACTGAGAAACACATTGCCAGAGTACTCGTGCGGATTAACTTTATTGGCGCCCCTTTCCTAACGGTGATTGCCATTATTCCTTACATAGTGTCAGCCTTAATCCACTTACCCAACTCCTTAAGTTTAGGGGGTACGGGGATTATTATCATTGTTACCGCTGTGGTTGAGTTTATGTCAGCACTCCGCTCTGCAGCCACCGCTACTAACTACCAACAACTGCGCCGCAACTTGGCGATTGAAGTGCAAAAAACAGCCCAACAAGACAAGGAGGAACAACTGCGCGCTGAAACCCCTGGTATTGGTAACCTCTGGTAATGGAAAATAAGTTCTTGTTTTTAGGCGCTCCGGGTGTGGGTAAGGGTACACTAGCTAAGCAAGTAGCCAACACCACCGGATTGTTTCACCTTTCCACTGGTGACATCTTTCGCAGTGTGATGCAAGAGCAGGGTGCGTTGAGTCAAACGCTAGCGCACTATATGAACCAGGGTTTGTATGTCCCTGATGAATTGACTAACCAAACCTTTTGGCACTTTGTCACTACCCACCAAAATGAACTCCACAAGGGCTTTATCCTAGATGGGTATCCCCGTACCTTAAACCAACTCGAGTTTTTGCAAAGCAAGTTGCAATTAGACCAGGTGTTTCACCTTAAGCTAAGTGATCCCCAAGTGCTAGTAGCGCGGATCTTAAACCGTTTGGTTTGTCCTAGTTGTGGTAGTGTATACAACAAGCAGAGCAAACCACCATTGAAGGCCAATCAGTGTGATCGTTGCCATGCTACGTTACAAGCGCGCAATGATGACACGGAAGCGGTGATCTTAAAGCGTTTAACGCTTTACGAAGACACCGTTAAACCCTTAATTGAGGCCTTCACCAAGCAGGGCATTCTCACCGTCATTGAAGCCCAATTACCGCTCGAACAACAGGTCAATTTGGTTCAACAGTTAGTGCATTAATGGTTTATTTAAAGTCTGCGCGTGAGGTCGAACAGATCCGCCAAGCATGTAAGATTTTCCAAGAAGCAAAGGCATATTTTACAATTGAAAGGCTACTTGGCAAATCTTTAACAGCAATTGATCAAGCGTTAAAGCAGTTTATTGAGAGCAAAGGTGCAACTTGTGCCTTTCATAAATACCAAAATTTCCCGGGATTTAACTGCCTTTCGTTAAACGAAACGGTAATCCATGGCATAGCGGACAACCGTGTTTTTGGTGTTAAGGATAAACTGACGTTGGATATTGGCATTAACCTTAACGGTTATATTTGCGATGCAGCTTTTACGGTTTTGGGCCCCAAGGCTCCAGAGCCCATGCAAACGCTGTTAGAGGTAACGGAAGCTTGTTTTACTGCTGTGGTGGAGCCTCAACTGCGCCCCAATAACCCCACTGGTAATGTTTCCCATGCCATCCAAACCTACTTTGAATCTAAGGGTTATTACCTTTTAAAACAGTTTGGTGGTCATGGTTGTGGGATTAAGGTGCACGAAGAACCGCTGATTTTGAACTATGGTAAGCCTGATACAGGTACTAAGTTAGAACCAGGGATGGTCTTGTGCATTGAGCCCATGGTAATGACTGACAGTGATGCAATGGTAATGCATAACAACAGCTGGAATGTATTAACCCCTAAAAGCCGTTACAATTGTCATGTAGAACAGATGTATGTGATCACCACAAGTGGATTTGAATGTTTAACTAATTAATGCAACCAAAGTTTAATAACCAAGCAAAGCAGGACAAACTCGTCTTAACGGGCAAAATCTTGGAAATCATCCACGGTGACAAGTTTCGCGTGTTGTTGGAAAACAACGTTGAAGTAGATGCTCACTTAGCGGGCAAAATGCGAATGCGCCGGTTACGAATTTTACCGGGCGATTTAGTTGAAGTGGAATTTTCCCCTTATGATTTAAAGTTAGGTCGAATAATAGGTAGAAAATAATTAAAATACTATGAAAGTTAGAGCGAGCGTAAAGCCAATTTGCAAAGATTGCAAGATCATTAAGCGCCACCAAATTGTGCGCGTTATTTGCAAAACTCAAAAACATAAACAACGACAAGGATAATGGCACGGATTTTAGGTATTGACATTCCCAACCAGAAACGCATAGAAATTGCGTTAACCTATATTTTTGGTATAGGGCTTTCGCGCTCCCAAGCCATCTTAAAGCAAGCGAACATTAACCCCGATAAACGGGTCAAAGACTTAACCGAAGAGGAGTTTGTTGCCATTAGGAATGTAGCTAGTGCTTACAAGATTGAGGGTGATTTGCGTCGTGAAATTGCACTCAACATTAAGCATTTGAGTGAAATTGGAGCATGAAGGGGCTTGCGCCACCGCAAGAACTTACCAGTGCGCGGCCAACGTACCCGTACCAATGCCCGCACCAGAAAGGGTCCACGTAAGACCGTGGCTAACAAGAAGATTGAAAGCAAGTAATGGCTAAGAAGAAAAAGATTAATGTATCTAGTGGAATTATCCACGTATCCTGTTCCCCAAATAACACAATTGTATCCGCTAGTGATCCGGGGGGCAACGTGTTGTGCTGAGCTAGCAGTGGTACCATGGGCTTTAAGGGATCGCGCAAAAAGACCCCGTACTCAGCCGGCATAGCAGCCGATAAGGTGGCCAAAACCGTCAAGGAAATGGGGATGGCGACCGTTAAGCTCTTTGTCAAGGGTACAGGTCGGGGTAAGGATACCGCCATTCGCAGCTTTGCTAATGCGGGACTTTCTATTACTGAAATTAACGAAAAGACCCCGATTCCCCACAACGGTTGCAAACCACCAAAACGACCACGCTAAGATTTATGGAAAAGTTTTTAAAGTACGAAATAAAGGTAAATAACGAACAAGCACGCGCTAACCCCAACTACGGCATCTTTGAAGTTGGACCACTCGAATCGGGCTTTGGCATTACCATCGGTAATGCTATGCGCCGGGTGCTGTTGAGCTGTATTCCAGGTGCCAGTGTGTTTGCACTCTCAATTTCGGGTGCCAAGCAAGAGTTTGCTGCTGTGGAAGGGATGAAGGAAGATGTCACTGAAGTGGTGTTAAACTTCAAGCAATTGGTAGTGAAGATTTCTGACCTTCTGTTTGAAGATGGTGAAATGGTCGAACCACCACTAGAACGTTGACCATTGTTAACGGTAACAGCTGAGAAAGCGGGACCGGTGTATGCGAAGGACTTGGAGTGCCCAGCTGGCTTTGAAGTGGTCAACAAGGACTTGTACCTGTTCTCGTTGCAAACTGACAAAAAGGTAACAGTTAACGTGTATGTGAAACAAGGACGGGGGTTTGTTACTTTCTTGGAAAACCGTGAAATGATTAATTCTTTGGGGATTATTGCCACTGACTCGAACTTCTCCCCGGTATTACACTGTGGTTATGAAGTACAGGAATTGAAAACTTCCAAGCAAAAGATTACTGATCACTTAACTTTCAAAATTGCTACTAACGGTGCCATATCCGCTGTGGATGCCTTTGCAATGGCTGCAAAGATCTTGATCGAACACCTCAATCCGATTGTTAATGTCAACGAATCGATTAAGGCGTTGAATATTATCCAAGAGAAAGCCGAAGAACGCCGTGTCCGTTCGTTTGCGAAGCAAATTGAAGAGTTGGACTTTACCGTGCGTACCTTTAACTGTTTGAAGCGTAGTGGGATCCACACCTTACAAGAGTTGTTGTCCAAGTCATTAGCTGATATTCGTGAAATCCGTAACCTCGGTAAGAAATCGGAACGCGAAATTATTAAGAAGGTACACGAATTAGGACTCAAATTAAGATCTTAGTTATGTCATACATTAATAAACCAGGTAAAACTAGTGCGTGAAGGGTCATGACCGTGCGCCAACAGGTAAGCGCTGTTTTGGCTTATGGCAAGATTGAAACAACCTTAAAGAAGGCCAAGAACACCCAAAAGCGTTTAGATAAGTTAATTACCTTAGCTAAAGTTGATAACTTTAATAACCGTCGTCAAGTCAAAAAGTGGTTGTTAAACACCAACTTGTTCGATGTTGATCAACTAATGGATCACTTGTTTAGCAAAGTTGCCCCTAAATACGAAAAAACCCCCGGTGGTTACAGCCGTGTTTTAAAATTAGGGCCACGTCGCGGAGATGCCACGGAAATGGCCATCTTGCAACTAACTGATGCTAAATACAAATAAACAAGCAGCTGCAAGCTTTCAAGGTGTTTATTTTTCTTACAGCGAATTACCATTAATTCGCAACCTCTCCTTTGATGTTTTCGAAGGGGAGTATGTTTGCATAGTTGGCCATAACGGCTCGGGTAAATCCACCATCTCGAAGCTGTTAACGGGCTTGTTAAAACCACAAGCGGGTACCATCAAAATCTTTGGTAAGACGATTTCGAGTGAAAACGTGACTTACCTACGCAACCACATTGGCATTATCTTCCAAAACCCCGATAACCAGTTTATTGGTATTACCGTGGAAGATGACATTGCTTTTGGACTGGAAAACCGCCGTTATACGCGCGAAAAGATGAAGAGCATCATTGAGGATGTAGCCCAACAAGCGGGAATAACGGAGTTATTGCAAAAAGAACCGCATAACCTTTCGGGTGGGCAAAAGCAACGCGTTGCCATTGCCTCGGTGTTAGCCTTAAACCCAGCCATCATTATCTTTGATGAGTCGACTTCGATGTTGGATCCTAAAGCGAAGCGTACCATTAAGCAGTTCATGGTGGAACTGCGCAACCAGGGCAAGTGTGTTATCTCCATTACCCATGACATGGAAGAGGTTACCAAAGCAGACAAGGTTTTGGTCATGAATGAAGGTCGTTTAATTCGTCAAGGTAAACCCAAGGATGTCTTTAACAGTGCAGCCGAATTGCAAAAAATCCGCTTGGATATTCCCTTTTCTTTAAGCCTTTCCACGAAGGTTAATGGTGTGACTAGTACAATAGACTACCAACAACTAATTGAGGCCATTGGTAAGCTATGAAAAAAATAGTTGAACTCAAAAACCCCTTAAGGGATGGAGAAATCCTCAGTGTTAGTAACCTTTCGTGCTTTTTTAACGAAAAAACAGTCCATGAAGTCAAGGTAATTGACAACTTTAGCTATACCTTTGCTAAAAATAAGGTCTACTGCATTATTGGTGACAGTGGTAGTGGTAAATCAACCTTAGTCAACCATTTTAATGGTTTAATAAAGCCCGCTTACGGAGATATTTGGGTCAAGGATATTTACATTGGTCAAAAGCAACGCAAAATTAAGGACTTTAAACGCTTGCGCAAAACAATTTCGATTGTCTTTCAGTTTCCCGAATACCAACTGTTTAAGGACACAGTGGAAAAGGACATTATGTTCGGTCCAATTGCTTTGGGTCAATCGAAAAACGAAGCACGCCAAAAGGCAGCTTACTACCTCGAAAAAATGGGGTTAAAGTACACTTTTTTAGAGCGCAATCCGTTTGAGTTGAGCGGGGGGCAAAAGCGCCGTGTAGCGATTGCTGGGATTTTAGCAATTGAACCCGAAGTTTTAATCTTTGATGAACCTACCGCTGGACTAGATCCTGAAGGGGAACGAGAAATGATGCGTTTAATTAAGGATGCTAAAGCTAGTGGTCGCACCGTCTTTATGATTACCCACCAAATGGAAAATGTTTTGGAAGTAGCGGATGAAGTGCTGGTGTTGTCCAAGGGCCAACTCGTTAAATCCGGTGATCCTTACGAAGTTTTTATGGATGAAGCATTTTTGGCACACACCACCATTATTATGCCTCCAGTCATTCAAGTTATCAAAGACCTAATTAACTTAAACCCGAAGTTTAGTTGTTTATTAGACTTTAAACCGCGCAACTTAGACCAGTTGGCGGATGCCATTAACAACACAATAGCTCATGGATAGTTTTTTTATTAATGGTTATGTACCACGAGATACCTTTATCCACCGGCTCCACCCGACAACGAAACTGTTAATCTTTTTGCTGTTCGTGATCCTGGTGTTTGTACCCATTGGCTTTGTTTTTCAAAGTGTGATCTTTGTCTTTGCCACAGTTATTTTCTTTGTAGCCAAACTACCCGGACGGTTTTACCTCTCCTCGATTAAGTCAATCTCACTGTTATTCTTACTACTGTTATTTGTCAACTGGTTTACCTTCCGTGACCCCGGGTTCTACATTACCGCTGATCAGGTTAATACAGTAAAACCACACTTTAACGGTAATAACTTTAACTTCTGAAACATCTCCCTGTTTAACTATCAGGACAATGTGTTTTCGCAAGTGTTTAACTTTAACCGTGCCAACATGACAGAGTTAAACAAGATTAACTTCTTCTTTAAGGAAACAGCTAATGCTAATGCATACACCAAGGTAACGGGGATTGATAAATTGGCAGAAATGTTGGCTTCAAAGAATCTCTTTAAGTTCAACGGTTCTACCAATGGCATTGATAAAAACAAGATTCTTGGTGCCTTTCTAGATCATAAGATTGCCGTTTACCTCGGTCGTAGTTGGGGTGGTGACTTTAGTGGTTTTGTGATTGACGTAAGTGTAAGTGATAAAACATCTACCTTCACGATTAAACCCTTTTTAGCTAACTCGAACTATGTGTTGACACTGCGTGCCATTATTCTAGCCTTTTACGTTACCCAAAAGATCTTGATCATGATCATTTTGGCAACAGTGTTAACTTCCACTTCAAGCTCGGTTGAGTTGGCATACGGAATTGAACGCTTGCTGTGACCGTTGAAGCTGTTACGGGTTCCAGTCAATGTGTTTGCGATGACAATTGCAATTGCAATTCGCTTTGTCCCTTCTTTATTGTTGGAATCACAACGGATTTTAAATGCCCAAGCATCACGCGGTTTGGACTTTAAGAACGGCAATTTCTTTGTCAAAATGCGTTCATTGTCTTCCTTGGTTGTACCGATGATTTCGATTGCATTCCGCAATGCTGGGGAGTTGGCGAGTGCCATGGAAGCACGTGGTTATGATCCAACTAAGAAACGTACTACCTACCGCAAGTTTAAGATTGATTGGGTTGATGCCACAGCTTTAATTCTCACCGCTCTTTACTTTGTGGTGATTATTTTCTTAACCGTTAAGGGTGCAGTGTTCCTAGATTTAGGGACACCTGAGTGGTTGCTAACCGGCAAGATTAAAGAGCAGGTAGAGCGCTCATTATCAGTTAAAAGTGCCTAGGTACTTAGCGATTGTCGCTTACAATGGTGCTCCCTTTCGTGGTTGAGCCAAACAACCGGGTTACCACACCGTTCAGGGTCAAATAGAACAAAACCTAGCCTTAATTTACCAAACACCAATTAATATTTATGGTTCGGGTCGGACTGATAAGGGAGTGCATGCCATTAACCAAACCTTTCACGTTGATCTACCTGATAAGTTACCACTCAAACAGTTAATTGCCAAACTAAACCAATTAAACTACCCTGACATTTTTGTAAAGGCCTTAGTTCCAGTAGCACCGCGATTTCACGCGCGGTTTGCAGTAAAAACTAAGGTTTATGAATACCTTATTAACACCCGAGCTTTTAACCCAGCACAGTACCACACCGTGTGACAGTACAACCACCCATTGGATTTAGCACAACTTGAAGCGGATACCACGCTCTTTTTAGGCAGGAAGGATTTTTGGAGTTTTAGCACCGCTAGCCAAAAGGACACGCAGCGCACAATTAGTAAAATTATCGTGCAACAAGATCACACTGGTACGGTTAAACTGACCTTTTTTGGCAACGGTTTTTTAAGAAACCAGATTCGAATGATGGTAGCTTCTCTAGTGGCCTTAAACAATCGAACCCTGATGCGAGAAACAGTTCAACAGCTGTTTGATCAACCACAAAAGGGTGGGTGTAAGGTTAAAGCCCCCGCTTGTGGTCTTTATTTAAAAACTGTCTTATATGAATAACCAATACAACTGAAACCTCGAAGTTTTACTAAATGGTAAGAGTTTAGCTGATAACTTTACCGAACTCAAACAACTCAGTGAGCAGGAAAAAGCACTTTACGATGGTGGTGCTTGCTTTCAAACCAAAGCTAAGTTCACCGAATTTTTACAACTACAAGAAAAGATTGAGGTCTTAGAAAACCGTTACAGCAATTTTTTGAGTAACAAGCACGCTGAAAACAGTTTGGACAAAACGATTAACGACGCTTTATTTCAGTACGAAATGTTTAAAAGTGAACATGCCTTGGTGTTTGTTGACTTTGAAAAGAACCTGTTTAAGCACGAAAAGGTGATTCGCGCATACCTCCAAGACCCTGCTTTAAAACAATACCAAAGGGATTTTGAACTAGTATGACGGAACAAGAAACACCAAATTGATCCCGCTAGTCAAAAGTTGTTAGCGCAAATTAGTCCGGCATTAAACCAAGCGGACAAGATCTTTAATGTGTTATCTACCGCTGACCTAAACCTCCAACCAGTCGTATACAAGGGTAAAACTTACGTTATTAACGCGGTTAGTGATTATCAAAGTCTCTTGGAAAATAAGGATCGTGGCCTGCGTGAAGCTGCTTACAAAGTGTGGTTGGAAATTTACTGACCCACACGCAATACCTTGAGTGTGAGTTTAGTGGAAAACTACATCCAATTAGAAACCTTTGCTAAGTTAAAGAAACACCCCAATTACATTGCCAAAACAGCTTTTGATGATGAAATTGATGTGGCTTTCATTGACTTTGTTTATGAGCAAGTGGCTAGCTTTGCCCCGACCTTTAAAGCCTTTCAAAGTTTAAGGAAGCAAATTTACAAGCACGTTTTAAAACTAGACAAAGCCCAACCCTGGGACTTGTCGGTACCGTTGTTTAAGGCTAGTGGTGACTACACCATTGAACAAGCCCAAACTGATGCGCTTAAGATATTAGCACCTATGGGTAGTGAATACCTGGAAGTGGTAAAGGAAGCCTTTCGGGAGCGCTGAATTTCCTGGTTACCCGATAAGAACAAGTATACCGGGGCTTACTCAATTTCCAACGTTAAGGGTTTAGATCATTACTTCATTTTGATGAATTTTGATAAAACCCGCGCTTCATTAAATACCCTAGTCCATGAATTGGGTCACAGTGTCCATTCTTGGTATGCATCTAAGTACCAAACGCAAAACTTGGATCCTACAATTTTCTATGCTGAAATTGCTTCCATTTGTAACGAATTACTGTTGTGTTACCACGACATTATTAACTATGAAAACCGCAATCCGCAGCAGTTAATCCGTAGTTTAATGGAACAAATTAGCCACTTCTTTGGCGCTACCACGAGACAGTTAATGTTCTCTCAGTTTGAACAAGACACCTTAAAGTTAATTCAGCAAAACCAAAAGCCTGACTTTAAGACCCTAGTGGAAATTTATGGTAAGACCGCCATTAAGTACCAAGCTGCTAATGCTGACGCTATTACGAAAAAGCTCAAGCAAACCAAGTACCAAAAGTCATTGGCTTACATTACCTCCATTCCGCATTTTTACGCTGGTAACTTCTATGTTTACAAGTACGCCATTGGTCAAGTTGTGGGTACCTTAGTTGGTAAAAAACTGAGTGCTGGTGACAGTAATATGCTCGCTGCTTACAAGCGCTTCTTGTCTTCAGGCTCCACCTTACCACCACTAGAAACAATTAAATTGTTAGGGATTGACCTTACCCAACCAGAACCGTGACAAGAAGCCCATGCGGAGTTAAAGCGCTGGATTAAACTAGTACAAACAGCCTTTAAACAGCTGCAACACAAAAAGCGCTAGTGCATTACTTTAACCGCGCCAAAAAGGCAAAGAACAATGAATTTTATACCTTGTTTGAAGACATTGCCGCTGAAGTGGCATGTTATCCCAATGCCTTTAAGGGTAAGGTAGTGTTGTGTAATTGTAATGATGGTTACCAATCGAACTTCTGGCAGTTTTTTCAAAGTCAGTTTCATGCTTTAGGACTCAAAAAACTAGTTGCGATTGCTTTTAATCCACTTGGCAACAGTTATCAACTCAACTTCGATGGAAAGGAAATAAAGGAACTACCTTTAGCTGGTAATGGCAGCTTTGACAGTGCGGAAGCCATTGTTCTTTTAAAGCAAAGTGACATTGTGGTAACTAATCCACCGTTTAGCTTGTTTCAGGACTTTGTTTGTCTTTTAGCAGAGCATGGCAAACAGTTTTTGGTTTTAGGTCACAATGGTGCTGTCGGTTATAATCAGATCTTTAAGCTGTTTAAGGAAGAACAGTTGTGATATGGTCATACTGTTAACAGTTCGATGTTGTTCCAAGTGCAGTCTAACTTTAAACTGTATGACCCCAAAAGCGTTAACTTTGTTAAAAAGGATGGTCAACTGTTTCAAAAAGTACCGGGAATTTCCTGGTTTACAAACCTCAAGAAAAACCAACAACCAGCTTGACTAAAAACAAAATCACGCTATCAGGGCAACGAACACAAATACCCCAAGTTTGACTGGTATGATGCCATTTTTGTCAGTAAGGTTAAAGAAATTCCCCTAGACTGATTTGGTTACATGGGGGTGCCATTAACCTTTCTCAATTGCTTTAATCCCAAACAGTTTGAGCTAATTGATTGTCTAGCCAATCCCTACGCTACCCTAGACACATTAAAAACTAATGCTTATGTGAGATCACACCACGGTGATGTGAGGAATGTGAAGGGTAAGCGAAGGTATGTTAGGGTAGTGATTAAACAACGTCAAAATGTGATTTAATGCCTTAAGTTAAGTAATTTTTGCAAAAAATTTCCTTTAATTTGCTAAATTATTTTAAGGATAGCACACTGCTGTCAAACCAAATGAGAACGAATGAAATTCAAAATATTTTTGGGCAGCAGCTTTTTTGGTGTTGCCACCTTACTTAGTGCTTGTGGTACTGGTCGCTTTGATCAAATCGATGATGGCAAAATTAAGCTTGCTGTTGTCACATCCCCTAGTGCCGCTAGTTCACTGCAAACCTTATTAGATCAATACAACAACACTAAAGCACCAGCTGATTATCCAGTGGAAGTAGTTTCACTAGACAGTACTGGGAGCTATACTAAAGGGAAATTTGATACCCAAAAACGGTTAGCTGCTAAAGATAAAAATAACTTTTATAACCTAACCTTTAACTACCCCGACTTAGTTTCATCTTTAGCAATTAACGGAATGGAACTCAACCTTGATGGGGTTAATGTTTCTAACTTCGAACAGTCTTTCTTAGACTTCAACAAGAACATTAGTGGCGTACGCAAACCCGGCATTTACGCCTTACCAGCTACCATGAGTGGTGAAGTTTTGGTTTTAAATGGGCCAGTTCTCCACTACATCTTGAACAGTGCCAAGAAGAAGGATGGTACCGAATCAAAAATTAAAACTGCTCAATTAAAAACAGCTTCAACTCAGGCAGAAGTTAAGGGTACTATGACCATGGCTAGTGATGAACAAACCACAAAGCTTTGGACTAATATTCAAAACGCCGCTAAGGAAAATGCCGATAACGGTACAACTGAGAAAGCGGAAAAAACAGTTTCCGCTAGTTCGTTGCAATTAAAAAATACGAATAAAACCACCGAAGGTACCCTAAAAATTGGTACTGATGACAACACTAAAAATCTTTGAAAAAAGATTGAAGATGCCGCAAAAACTAACGGGGAAAAAGGTAATGAGAAGCAAGAAGCTACTCAAAGCAATGCTAGCGCTAGTCTCGTTAAGTTGGATCAAAAAAATACTAGTCAGGATAAAACTCAAAACACCCAGACTAGTGATGATGAAATTAAAAAATCTTGAGGCGAATATAAGGAAGTAGAAGGCGGTCTTAAGGGTTATGAATTTAAGGCTGATGTCTTTGAGAGTTGGGAAAAGTTAAACGATTTTGCAGTACGGGTAGCAAAGTCGTTTAGCAAGGTTTCTGAAGAGAAGAAAAGTGGTAGTGACATCCAGGGTGTTTTTGGCATTGGTAGTTTAGAAAATGCGCTTTACACTGCATCATTTGCAGCCGGTGGTGGCGACTATAACAACTTCTTGTTTAACATTAAAAAAGGTCGCGCTGATTTTAGTAACTTCTTTAACCACAATTCAAAAACTTTCCAAAGTCTAAGAGATATTTTCAACAGCTTTAAGCCTTTAATTGACCAAAAGGGTTTAATTAATAACAAACACTTTGATACTCCAGTGAATAATTACGCTAAATTTCACCAATTGGCATTCTATGTATCATCGACAGCTCGCTTTCCTTACTCGTTTGCCAAAGACAATGTAAAACGCTTAATTATTGGTAAACGCGAATTGGAAGTAAACCCTAAATCAATGTTTGCCATTAAGAAGGAAAACGGCAATAACGGTAACAGCAACCTCTTAGGTAAAGTTGCACTAGATAACAATAAATCTATTGAACTTTACGAAAATAATATTCCTAACGGTAAAACTGATGCCATCTTAATTAAGAACCAAACTTTAATTAGTGCGTTGAAGAATCCAAAACAGACAAAGAGCTCTCAAAGATCTTTTCAGTCTACAAACACCCAAAATGATGCCATTTGTTACTTAGCCTTCAACAGCAAGATTAGACCAGACGATAAAGACATTTTCCTATTAGACAAATTTGGTGAAAAATTTGTCACCGCAATTGTTAACTTTGAAGAAAAAACGGAGGTCAAAATTAATACGCTCCAAGAAAAGGAAGCTGTTGTTTTACCAGCACCACAAAAGTTTAAACCTACTGATCCAAAATCAGTGGCACTTGTTCAAGGTCCAAGTTTAATCGGTATTCACGCTAACGCTAACGAAGACCGTTCAACCATTAAGTTTCTCAACTGGTACCTTAATGCTGAAGTTGATTGAAAAGATGGAGAAAAGAAAACACCAGCAGAATACTTAGCAGAAAAAGCTTCTTACCTATTGCCGTTTAAAAAAGTATTAGAAAAATCCAAGCAAAACATAAAAGCGACCTCTAAGGAAGGAGAGCAAAATCAAGGCAAGAAAGGTGATGGTGCACAGAACCAAGGTAAGAAGGGCGATGGAGCTCAAAATGGTAAGAATGATAAAGCAAAGCACAATGGTTTTGTCGATATTGCCGTCAGCCAATTTTTAAACGGTTCTGTCGCTAAGTTTGTGGAACCAGCTGACTTTTTAGGTATTCGCTTACGTGATGCCGTTAAGAGTACCTTTAACGCTGCCATTAACAACACCTCAATTAACTTTGACAAGTTCATTCAGTACATTAACGATTCTTTAGGTAGCGACTTTAAGCAAAGACAGTAGTTTAAACTGATTCTTTACCTCTAAATAAAAATACTAAAGCCCATTAAAGCCGAACCACTAATTTTGCCCTAGCTTGTAAAGCAGTTAAATATTTTTTAAGATATCTTCACCTCTTTTTTGCGTTTAGTGATGAAATTTAAGTATGGTGCCATTGTTTTCAGTGGTCTTTTAGGAGTCTCTGCCATTTTAGCTGCTTGTGGTACACGCGGTAAATTTGACCAAATTGATGATGGCAAAATTAAGCTAGCATCTTCGTTAACTTCCAAAGGTGCCGCTAATGCTTTACAAGCAATTGTGAAGAAGTATAACGAGGTTAAAAAGCCTGGTGATTACCCAATTGAAATAACCCAAATTGCGGGTGGTTACGACCAAGCACGCGTTGACTTACAATCACGGGTTGGTGTTAAGGATAAAACCAATTTTTACAACCTCATTTTGAACTATCCCGATGTAGTTTCAGTGCTAGCACGTAACCAAATGGAACTGCCATTTGATGGTGTTGATGTTTCTAAAATTTCACCTAACTTTTTAAATTTTAACGAACGTATTAGCGGAGTTTCTAAAAAGGCTAACTATGCCATTCCGGTCTCTGTTTCGACTGATATTTTAGTGCTCAATGGACCAGTTTTGCACTACATTTTAAACAGTGCTAAAGGTGAAAGTAAGGGCGCACAAAAAGATAACAAGAGTGCTGAAGTTCAAAGAAAAAGTACAGGTCAAAAGACCGTTACCCAACCTTTAACTATTGCAACTGATTCTGCTACAAACGGTCTTTGGAAAAAGATTGAAGATGCTGCAAAAGTCAACGGTAAAAAGAAAGAAGAGAAAAAGAGTACACGCAGTAAAAGAGCCACAGAAGGCACACAAACCACCAAAGAAAATACGGGTGGTGATGCTGCAACAAGTGATACCAAAATTAAAGAATCTTGAGGTGCTTACCAAGAAGTAGAAGGCGGCTTAAAGGGTTATCAATTTAAAGCCATTGTTTTTGAAAACTGACACGATCTAATTGATTTTTCTACCAGAGTAGCTAAATCATTTAGCAAGGTCAAAGATAACAGCAACAAGAAAGGCAATGAAATTCAAGGTGTGTTAGGTGTTGATAATTCACCTAATGCTTTACTATCTTCGGTGTTTGCTGCTGGTAACAGTGACTATAACAACTTCTTTTATAAGGTGCAAAATGGTCGGGCTGACTTTAGCAACTTTAACAATAAAGGCAGTTCTTATCAAAACCTTAAAAATGTCTTTAACGACTATAAGAACCTAATTGCACAAAATGGTCTTTATGTAAATAAAGGCGGCTCTTACTCTTCAAACTTCCAAAAGTTTCACCAGTTAGCTTACAGCATTTCCTCTACTTCTGGTTTTGCTTATTCGTTTGCTGGACAAAATTCCAAACGTTTTAAATTTACTGATGATGGTACTTTTGTTGAGTATCCTAGTTATACAACTGAAGTTAATGCACCAGAAAGTAATAATGGCAATGATGGAAAGCAACAAGGTCAAAGTGATCAAGGAAACCTTTTAGGAACTTTTGAAGTTGTAGATAAAAGTACTAGTGATATAAAGGTGAAACCTAAAACTCAAGCGGAATCAAAGAAAAGTAGTGATAGTAAACAAACTGCAAACACAGGCAAAGGCAGTAATTCAAAACAACAAACTCCGAAGAAAACTATTTCGCTTTATAAAACCAAAATTCCTCAAGATAAAACAGAAAACGTTGACGCTTTTTTAGTTACTGATTCAGAATTAATTAGCAAACTTGAAAAAGCTAAAAATAAAAAGGAAGAAACTAAAGCTTCAGGTAAATCTGCATCTGCACGTGTGGCAGTTCAAGCAACTCAAAAGAAACAGTCTAACGAAAAGCAGATAGTTGGTTACACTACTACATCCGCATTAAGCGAAGACGGCAAACACATTTTTAAATTGGGTAAACTCAACAGCGAGAACTATGAGCGCAAAATCATTGTTGGTGCTACTGTAGAAACACTAGAACAATCCACTACCTTGCAAAGTGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAGTACAAGGATAGTGATCAAAAGAGAGTTATGATCACCCAAGGTCCTAACCTAATAGGGGTTCATGCTAATACTAAAGAAAATGAAGAGACTAAGAAATTTGTTAACTGGTTTTTAAATAAAACCGAAAGTTGAGAAGTTAAAGGAAACGGAAAGGATTCACAAACTACTAAATCACTTACACCAGCGCAATATTTTGCTGAATCAGCATCTTATATTTTGCCGCTAAAGGAAACTGTTGAAAAAGATCATAAAGAACAAACTAATAAAAACACCTATGTTGCCAAAGCTTTAGAAATGCTCAAGGAAGTTAGTGAAAACAAATCAGTCAGCTATAGTGATCCGAGTGATTTTAGAAGTGGTAGATTCCGTGATGCACTTGGTGCTAACTTTAATGCCACTATTAATTCAAAAGCAAATTTTGAAAAGTTTTTCCAAGGTTTTAAAGCTGCATTAGGCTCTGATTTTGATAAATAGAATAGAAGTTTTTAGTTGCTTTTTAGATAAAAATTTCAAATATTTCTCCTTTATAAAACAATCTTTCAAAAAGCTTTTTAGAAAGAAATTAAACTGTTACTGTCGTGGAGATTAAAACTTACAAAATCAAAGATATTTGTGATATTACACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGAAAGTAAAAAATAAAGAAATTAGTTCAAAATATCTTGCTCATATTTTGAAACTAGAAGCACCGAAATTCACAAACCGTGTTTTTAAAAATAGACCTAAGTTAACACATAAAACAATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGGGCGCGCAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTTAATCAAGAAAATATCCGAAAAATTTATGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACACATTAAAGATTGCGACTTTGATGGTGAATACATCACTTGAACTACAAATGGTTATGCTGGAGTAGTTTTTTATCGTAATGGCAAGTTTAATGCATCACAAGATTGCGGTGTTTTAAAAGTTAAAAATAAAAAAATCTGCACCAAATTCCTATCCCTTTTATTAAAGATAGAAGCGCCAAAATTCGTTCATAATCTAGCTTCAAGACCAAAATTAAGCCAAAAAGTAATGGCTGAAATCGAACTCTCTTTCCCACCTCTAGAAATCCAAGAAAAGATTGCCGATATTCTCTTTGCTTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTTCAAAAAATAAGAAATTAGAAAGTCTCAAATCTCTTTAATAATAAAAGTTAGTTCAGCAAACCACAAACTGATCCCACTTCTCCCCCACGCTCACCCGCTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTGCTCGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGTACAGGTATGGCAATAACCACCGGGGCGTGGATGATATAACCGCGCCTCAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGTCTCAAAGCCAACGTCCAAGCCACGCTCGGTGGCAGTCAGACGACGACTACAGGCGGCTCCAAATGATCCACCCTCGACAAAGCCAACCTCCAGCCCTGAACGGGGGCGGGGTGAAGGAATGATAAGACTACTGGGAATAACACCCAAGGCAACGAAAACCACACCACCTTTAAAAACGCTACGGGGATGGGCAGTCAAAACCAGGGTTCTACAACAACTACCTCAGCGGGCAATCCCGATTCTTTAGTTACTGATAAAGTTGATCAAAAAGGTCAGGTGCAAACAAGTGGTCAAAATTTAAGTGAGACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACATCCGACTGACCGAACGCGCTCAGCTTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCACGGCTTGTTGGGCAGCATCCCGGTGTTGGTTAATAAGAGTGGCGAAAATAACGAAAAATTCCAAGCCACCGACCAAAAATGGTCCTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTATGGTGAGGTGAATGGGCTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGTACGTCTAGCACTGCGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTTGGTAACCTCGTTGTCAGTGGCACCAGCTTCAGCTTCCAGCTCGGCGGGTGGCTGGTCAGCTTCACGGACTTTGTCAAACCCCGCACGGGTTACCTCGGACTCCAGTTAACGGGCTTGGATGAAAGTGATGCGACGCAGCGCGCCCTCATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGTTGGGCCGCGTGGAGAGTGTGTGGGATTTGAAGGGGGTGTGGGCGGATCAAGCTCAGTCCGACTCGCAAGGATCTACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCTTTGGCCTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGACCAAACCCAATCCACTAACAGTTCCCCCTACCTGCACTTGGTGAAGCCTAAGAAAGTTACCAAATCCGACAAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGCGCCAGAATGCTCAAATCATTTGGTACAGAAAATTTCACCCAGCCCCAACCCCAACCCCAAGCCCTCAAAACAACGACACCGGTATTTGGGACGAGTAGTGGTAACCTCGGTAGTGTGCTTAGTGGTGGGGGGTACCACGCAGGGCTCAAGCACCACCAATCAACTGTCACCCGTTCAACGGGTGAGTGGGTGGACCGCTAACTATTAATAGGAACTTTTCTTATTAAGAAGAACTTTTTTGAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTTAACGAATACCCAAAAAGTATGAAATTGATTAAATTAGCGAGGTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTTCTTTGTTTAGCCAAAACTTTTTGCAAGTTTTGTTGACACCTTTAAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAAAAGGAGGTATTACTTAATGAAAGAAAAAACTCCCTTTAAAAACGAAAAAGAATTCCATGAAATGGTGAAAAAGACCAAAAAGGGCACTTTTTCAGGTTGATATATCATTGACAAAGATAACAACAGTGTGGAATTCTCTGGCAAATTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAGTTAAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAGCAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGCTTGGACAAAATTGATCCATCTAAATAGTTTTTAGGCTAATAACAAAAGTGCCAGCAGTTAATCTTAAAGATTAGTTGCTGGTTTTTTTCTTCACAACCAATAAACAAATAAAAAGCCATCCACCGCGAGGTGAACGGTAGATGGCTAAAAATAGTTTTTAATGATTTTGAAAACTAACGAAAAGTGTTAGTTTGAGTTGTAAAGCAGTTATGAATGTTTGTTCCTTTAAAAATTTGTTTTTTGTTTAGCTAAATATGTGCTGAAAGCCATTGCGTTAACATAAGTAAATTAAGACATAAAAAATGAACACATTAATCAAATAAGTGTGGGAACAGAAAATCAGGCTTTCAACACAAAATTTTCGTTTTTCTGCTCTCACTAAATAAATTGAGTTGGGTTTTAAAAACCGCCAAAAATTTTTAATTTAGTTTTTGCTACTTTCTCATCCCTCCCCCTCACGGGTGAAAACCCCGGGGCGTGGGCCTTAGTGCGCGATAACACTGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCTGCGCGAACCGAGGCCACCTTGACCACCACCACCTTTGCGCTGCGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGACTTTTCGAAGTTAAACCCACAAACGCCAACGCGTGATGCCAACGGCCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGCAGTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCCCAAGACCCCACCGATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGTGTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCACCCAACGTGGTTCGACCAATTCCGACACCAAAGTCAAGGCTTTAAAAATAGAGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGCTGCAGTTAGAAAAAAATGATCTCGCCAACGCTCCCATTAAGCGGGGTGAGGAGTCGGGTCAGTCCGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCGGCAACTCCAACCCCGGTTCCCCCACCCCCTGAAGGCCGTGGCTTGCGACTGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAACCGTACCGCCATTGACCGCGTTGATCACTTGGATCCCAAGGCCATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGTTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGACCAGGGCCAAGCGGTCGCGGATAATACCCAAACCGGGTTTGATGAAAGTAATACTTCGAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCCATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGTGGCAATGGCCATGTTACCAAGTCGGCCCACACCGCGCCTTTGAGTATTTGGCTTTATATATATTTAGTAAAGGCAGTTACTTTTAGGTTGCTTTTAGCTAACTCTTTATTATCAAAAAGCAACATATATAAAAAAACAGCTAATTAAAGCTAGTAGAGCTAAGCGATTATTTCTTTTAAGTTAAATCTTAGTTGCTCAAGATTAAAACGCACTAAATTGAATGCTGTTGTAATAAGGCGTTTTTTCAAATCAAATTCATACACTAAAAGTGCGAAAAGGTTGTATACGCATGGAAAATGCGCGAGTTCTCTTAATTGTTTTCGGTATCGTACTAATTTTTGTTGGTATTGGCTTTTTATTTTGAGCATTAAGAATTACGCTTAAGCGTCAAAAAGGAGGCGAAGCTACTACTAGCTTAACGACTAACGATCCTGCTTTAAAGAACTTTGGTCGGCCAAGTTGATGGCAACGTAATAATCAACTTGTGCTCTTCCTCTTAGCGACAGTATTTTTGATGTTGGGAATAGGCGGTCTTTCTTCCATTCCCTCAATGTTTAAGTAACAAAAATTTAAGGATTTAAAAAAGGTTCATTTATGCAAATTAAAGCACAGGACACAGGACAGCAAAAGAAGTCCTGTTTATTGTCTAACATCCGCAATAAATGGAAAAACCGGAATCGCGGGAGTTTCCGCCAATGAGTTGGAAAGCTTTCCAATGGGTTGATGATCCCGATTGCCGTTTTACCTATTGCGGGTATTTTTTTGGGTGTTGGTGATGCAATTGCCGGAAATGCAGGTGATTTAACTGGTCTGCGTTATTTCGGACTTTTCATTAAAAATGGTGGTGATGTTGTTTTTGCTAACCTACCCATTTTGTTTGCTATTGCTATAGCAATAACGTTCTCACAAGACGCTGGGGTCGCCGGATTTTCGGCCTTTGTCTTTTGAGCTGCTATGAACGGGTTTATGAGCTCGTTAATATTGCCTTTTGATAAAGCAGGCAAAATTATTACTGACACTTCGACTCCAATTGCTGGTTTTAAAGTACTTTACTACAAGAGTGTCCCTGTTCATGCTATTGCTACAACCTTGGGACTGCGAACATTATCAACCTCAGTATTCGGTGGGATTATTGTAGGTGCCTTAACTTCAGTACTTTACAAGAAGTTTTATGCCATTAGGTTACCTGATGTAATTGGATTCTTTAGCGGTACTCGTTTTGTCCCTATTATTTGTTTTGTGGTAGCGATTCCTGTTGCGTTAATCTTGTTAATGATCTGACCAGCTGTCTCCATTGGACTTAATGCTATTGGTACTGGTTTAGGTTTCTTAGGTGGTAAAGGTTATGGTGCCAACAGTTTGATCTTTGGTTACATTGAACGTTCCTTAATTCCGTTTGGTGTGCACCACGCCTTCTATGCACCACTTTGATACACCTCTGCTGGTGGTAGCTTACAAGAAATTGTTAACCAACAGGTTTGGATTCGTCCCGACTTCCACTTAAGTGATAACTATGTAGCACGGGTAATTGGTTGAGTTGATCCTAATAACTCTAGCATGTACATTATTCCTGGTGCATTAAATGGTCAAAATGGAAGTTCAACCGGTAATACAATGTCAAAAGATCTTAATGGAGCACTTTCTGCCTACATGAGTAAAGAAAGCACAGCTTTCTTAACTTGAAAAGATCTAGTTGATGGCTTAACCTTTAAAGGTAATTTTGATAAGATGGCCGAAAACGGTTTGTTAGACGGTTCTAACAAAATTTGATTGGGTCTGAACGGTTCTGGTATTTTAGGTAAAAAATTGTTACTAAGTGATGGTAATGTTTACACTATTACCTTTAAAACCTTTGCCAACACTACTCCAATTGCTTGGAGTAAAGGTGCACAGGCAGTGTTACCTTTAAATGCTAGCTCTACAATCGTTAACAATCCAACCGCTTTAGCAGCAGCTACACAAAGTAATAATAACACTAACAACATTAAACTTTACCCGGTAAATTCCTTTAGGGTAGCTGTTGAAAGTTTAAATCCAGCGCAATACTCCCAGGGTAAATTCCCCTTCATGTTGTTTGGAATTCCAGCAGCTGGTGTAGCCATGATCTTGGCAGCACCTAAAGATAGAAGAAAGGAAGCAGCTTCGATTGTTGGTAGTGCAGCATTTACCAGCTTCTTAACTGGTATTACTGAACCATTTGAATTTACCTTCTTGTTCTTAGCGCCTTGATTATTCTATGGGGTGCACGCTGTTTTGGCAGCTGTAAGCTTCTGGTTAATGAACATCTTGGGTGCTAATGTGGGTCAAACCTTCTCTGGTTCCTTTATTGACTTTATTCTCTATGGTGCTTTACCAGATGGTCGTCGTTGGTTAGCAAACTCTTACTTAGTACCTATTATTGGACTGTTCTTAGCTGCCATCTACTTCCCAACCTTCTACTTCTTAATTAAGCACTTTAACTTGGCAACTCCAGGTCGTGGTGGTAAGTTAATTACCAAGAAGGAATACTTAGCAAGCAAAGCAGCCGCTAAAGCAGAGGGTGTAAGTGGTGTAGCAGAAAACTTTACCCAAACGCAAATTGAAGCGGGCATCTTACTCCAAGCTTACGGTGGCAAGGAAAACATTGTTGAACTAGGTGCATGTATTACCAAGTTACGGGTTACTGTTAAGAACCCCGAACTAGTTAAGGAAGAACCTATTAAAGAACTTGGAGCTGCTGGGGTAATGCGTACCACCCCAACCTTCTTTGTAGCCGTTTTTGGTACCCGCGCTGCCGTTTACAAATCTGCTATGCAGGACATTATCCAGGGCAAGGTTAACTGGGAAGCGTTGCAAAAGGTTATTAATACAGATCAACTAGCTGTTGAGCCTAAAGAAACCACTCCACCAAAGGAAGTAATGCCGGTGGTACAAGACGAAATTGTGATTCTGTCCCCAGTTAACGGTACCTTAAAATCACTCAATCAAGTACCCGATGAAACCTTTAAGCAAAAGCTAGTGGGTGAAGGTGTTGCCATTGTACCGAGCGATGGTCACTTTAAAGCCCCTGGTGAAGCAGGTGTGAAAACGGAGTTAGCTTTCCCTGGTGGTCACGCTTACATCTTTGACATTGATGGCATTAAGGTAATGTTGCACATTGGGATTGACACTGTGCAAATTAACGCCAAAAAACAACCGGGCGAACCACTAGAAGTGTTTGACATTAAGACCAAACAGGGTGAGTACACTAAGGAAAAGAGTGAAAGTGTAGTGGAAGTTGATTTAAAGAAACTCAGCAAGAAGTACAACCCAATTACCCCATTTGTGGTAATGAAGGAATCACTAGAAAACTTTAAATTAGTGCCAATCCGCCAACGCGGTGAGATTAAAGTGGGCCAACCAATCTTTAAACTAGTCTACAAGAAGTCACAGGCATAAAAACTGCTTGGCAAACAAAGAGGTCATAGGTAAGCCTATGGCCTTTTTGTTTATAATGAAAGTCCGGGGAAGGCTTTAGATGAATTTTTCCCTGGTGTTTCATCTGGTTATCTTTTATAAGTTTTAGCACCTGCAATTTTTTATGTTCTTGCACGGGTGTAGCAAACATCTTTTAAGTTTTTTCATTCTTCAACTTTAATTTCTTCAACAGTTTTCTAGTAGTTGCTTATTTTTCAAACTTAAGTTAGTTAGTTAAGTATTGCCTCTTTTTTTGAAGCAGCTACACTTATCAAGTTACTCATATTTTTCTTTTTCATATTTAGACATAGGTTGTGTTAAGGGATTTTTAGGACTATCCATGTAAATACATCTGATAACTTATTTTGATTTTGTTAGCAATAACAACAAAATCCACATCTAAAGCAAACCAAAAAGTCACCTGTTTTTAACGGGTGGCTTTTTAATTGTGGAACTGCAAGCTCCTTAATAATTCTTATTAAGGAGGATGACAATGTTTGGCTTAACACTTTGGCACAAAAATGGTGTTGGTTAGCAATCATTAATTAAGTTAACTTGCTCTGTTTGGTGCATAATGTAAAATTACAAAGCATGTCAGAACTGATTACAACACCTGTAGAAACCACCGCCAAAGCGGAACTCGTTTCCCTAGCTAAGCTCGGGGAAATGCGTACCCATGTCGGAATGGTTAAGCGTTACTGGAACCCCAAAATGGGCTTTTTTATTGAACCAGAACGCAAGCACAATAACGATCACTTTGTATTAGAGTTACAACGTCAATCTTTGCAAACAGCTTACAACTACGTCAAGGAAGTAGCACAAAACAACGGTCAAATTCTCTTCGTTGGTACTAAGAACGATTATGTGAAAAAACTCGTTAATAATATCGCTAAACGGGTTCATGTTGCTTTTATTACTCAGCGCTGATTGGGTGGTACTTTAACTAACTTTAAAACACTTTCAATTTCAATTAATAAACTCAATAAGTTAGTGGAAAAACAAGCGGAAAACGCGGCTGATTTAACTAAAAAGGAAAACTTGATGCTTTCCCGCGAAATTGAACGCTTGGAGAAGTTCTTTGGCGGGGTAAAATCACTTAAACGTTTACCGAACTTACTAATTGTGGATGACCCGGTATACGAAAAGAATGCGGTAGCGGAGGCTAACAGCTTGAGAATTCCTGTAGTAGCGTTGTGTAACACTAACACCAATCCGGAATTAGTTGACTTTATTATTCCCGCTAACAACCACCAACCCCAATCAACCTGCTTGTTGATGAACTTATTAGCTGATGCGGTGGCTGAAGCTAAGGCAATGCCAACCATGTTTGCTTACAAACCGGATGAGGAAATTCAAATTGAAATTCCCCAAAAACAAGAGGCACCACGCCAAGTTGTAAACCGTGCAAATTCTAAACAAATTACCTCTCAACGGTTAAACATTACTCGCAACCCAGAAGTTCTCACCCGTGAATAAGTGAACTGGGTTGAGTGCTGCGGCCGTTTTAGAAAGCCGCGCACAGCATGGTGCTAATTTAATTCCTACAAAAAAATTAACCCCTTTTTGACTACTGTTCTTAGAACAGTTCAAGAGCTTAGTGGTGATCTTACTGCTGGTAGCTACCATCCTTTCACTAGTGGTTGCCATTATTAGTGGGGTTAATGCTAACTGGTTGTTTGACCACAACCTTGTTATTGAGTGAACCCAACCGTTTGTAATTTTGATAACGGTGTTGGCCAACTCCCTGATTGGCTCCATTCAGGAATTTAAAGCGCAAAAGTCAGCTCACACCTTAAAGTCCCTAACGCAACCCTTTACGCGGGTATTCCGTGAAGAAGGCTTGGTTTCGTTACCGGTGGGTGAAGTTGTTGTCGGTGACATTATCTTTCTGGAAGCGGGTGATATTATTCCCGCTGACGGGAAGGTGCTGCAAGCGAACCACCTCCGCTGTATGGAGAGCTTTTTAACTGGTGAATCAGTCCCAGTTGATAAAAGTGTGGTTAACACCGGTGGCAAGGGTTTGTTGGAACAAACAAACCTACTCTTTTCTGGTGCCCAAGTGGTCTTTGGTAGTGGTGTATTCGAAGTAACTGCTGTGGGGTTAAATACCCAAGTCGGTCAAATTGTCAAAACAGTGGACAGTAGTGCAACTAAGCTATCGCCCCTACAGCAAAAACTGGAAAAAGTGGGGAAGTGGTTTAGCTGGTTTGGCCTTGGTTTATTCGTTGTCGTTTTCCTAGTGCAGTTAGGGTTACTTGGTTTTCACAACTTTAGCGCTAACTGATCGATTGCCTTAATTGGTGCAATTGCCTTAGTAGTCGCTATTATCCCTGAGGGCTTAGTGACCTTTATTAACGTCATTTTTGCCTTAAGTGTGCAGAAGCTCACCAAACAAAAGGCAATTATTAAGTACCTAGCTGCAATCGAAACACTCGGTGGCGTGCAAATCATTTGTACCGACAAAACTGGTACTTTAACACAAAACAAGATGAAGGTCGTGGACTATTTCTGTTTTAGTAACACTACCCAAACAGACTTAGCACGTGCTTTGTGTTTGTGCAACAATGCTACAGTCAACACTAACGGAGACAGCACCGGTGATCCAACTGAAATTGCCCTTTTGCAATGGTTGGACCGTGATGGTTTGGAATTAAACCACTACACACGCGTGTACGAAAAGGCATTTGATTCCAACCGCAAGCTTATGAGTGTGGTGGTGCAAAAGGACAACCGTTTTATTGTGATTGTCAAGGGCGCACACGATGTCTTGTTACCGTTATGTAAGGGCTTGGATAGCAACCAAATTAAGCCTTTAATTGATGAACGTGCTAGCAACGGCTTGCGCAACTTAGCGGTGGGACTCAAGGTTTTATACTGCTTTGATCCAGAAAATACCCAAACTGTCAACGAATTGGAAAGCGAACTCGACTTTTTGGGTTTTGTTAGCTTACAAGATCCACCACGAATTGAAAGTAAAGCTGCCATTATGGCGTGTCACCAAGCCAACATTACCCCAATTATGATCACTGGTGATCACCTCAAAACCGCTACTGCAATTGCCAAAGAATTGGGCATTCTGACCGATGAAAGGCAAGCCATTTTAGGAGTCGATTTAGACCCCGCTAAAATTATGGAGTACCGCGTCTTTGCCAGAGTAACGCCGCAGCAAAAGCTAGAAATAGTTAATGCCTGAAAGCAAGCGGGTTACACGGTAGCCGTTACTGGTGATGGGGTCAATGATGCGCCCGCTTTAGTGACTAGTGATGTTGGTTGTTGTATGGGACAAACGGGGGTTGACATTGCCAAAGATGCCGCTGATGTCATTATTAGCGATGACAACTTTGCCACAATTGTTAACGGGATTGAACAGGGTCGTAAAACCTTTTTGACCTGTAAACGGGTGTTGTTTAACCTCTTTTTAACCTCAATTGCTGGCACGATTGTCGTGTTATTGGGTTTATTTGTATTAGGGGAAGTCTTTCGTGAACAACTCAGCAAAGCAAACCATAACTTCCAGGTCTTTACCCCTACGCAACTGCTAATTATTAACCTCTTTGTTCACGGCTTTCCCGCTGTGGCCTTAGCGATCCAACCCGTCCAGGAAAAACTGATGTTAAAACCGTTTTCCACCAAAAATCTGTTTTACAACCGTGGCGGGTTCGATTTAATCTGACAATCCTTGCTGTTGAGCTTTTTAACCCTACTTTTCTACAGTTTAGGGATGGTCTATGCCATTAATGATCCGGAGTTAGGCAAATCCGGTGATTTAATTAACCGGGCTGGTGCGACCTGTGGTTTTATGGTGTTAGGGGGCAGTGCAGCTTTAAATTCCTTAAACCTAATGGTGGATCGACCATTGGTAGCTACTAACCCAAAGCACTATGGCATTGTGTGACTAGGGGCGTTGTCCTCGATCTTTGTCTTCTTATTAATTATTTTCATTAACCCCTTGGGGTTAGTGTTTAGTACATTAAAAGACCTTACTGCCCACCCGGTTTTAATTGGTTATAGCTTTGGGGGCGTGCTTTTATATATGACAATTAACGAAGTTGTTAAACTTATTAGGTTAAGTTATGGGTCTGTTTAACTTCTTGAAGTTAGTCTCCCCGCGTCATCGGATTTACCATAAGGCAAGTAAGATTGCCAACGAAGTCGAGGGACACAAAAATTACTACCGCAACCTCACTGATGTACAGCTGCTGGAAGAGTCCAATAAGCTAGTGGATTTAGTGACAAAGCAAAACTACACCATTTTGGATGTGGCGGTGGCTGCTTTGGCCTTAATTCGCGAAGTGGTCTACCGTGAAACCGGGGAGTTTGCTTACAGAGTGCAAATTATTGGTGCTTACATCGTCTTGATCGGTGACTTTGCTGAAATGATGACTGGGGAAGGGAAAACCCTGACCATTGTTTTAGCAGCCTATGTCTCGGCGTTAGAAAAACGCGGTGTCCATGTGGTTACGGTCAATGAATACCTAGCGCAGCGGGATGCTACTAACGCCACCAAGATCTTAAAGCGTGTTGGCATGACCGTTGGTTGTAACTTTGCTAATCTAGCACCACATTTAAAGCAAGCCGCGTTTGCTTGTGATGTAACTTACACCACTAACAGTGAACTCGGTTTTGATTATCTCAGGGACAATATGGTGCACCGCTTTGAGGACAAGAAGATCCGCGAGTTGCACTTTGCGATAGTAGACGAAGGCGATTCGGTTCTCATTGATGAAGCGCGCACCCCTTTAATTATTTCTGGTCCTGCCAAAAACGAGTTTGCAGCATACGTGGCTGTTGATCGCTTTGTCAAAAAGCTCAAGGAAGACGAGTACAAGATTGACCCCGAATCACGTGCCCCCGCTTTAACAGAACTTGGCATTAAGCACGCGGAAAAGAACTTTAAAACCGATAACCTGTTTGCGCTAGAAAACAGTGACCTGTTCCACAAGATTATTAACGCTTTAACCGCCGTTAAAGTTTTTGAGCAGGGCAAGGAGTACATTGTGCGTGATGGTAAGGTGTTAATTGTGGACCACTTTACTGGCCGAATCCTGGAGGGCAGGAGCTACAGTAATGGGCTCCACCAAGCGGTTCAGGCCAAGGAAATGGTCGAAATTGAACCAGAAAATGTGATAGTAGCTACGATTACCTACCAATCGTTTTTCCGTCTGTACAACCGGTTGTCAGCGGTATCGGGTACCGCTTTTACTGAATCGGAGGAATTCCTCAAGATTTACAACATGGTGGTAGTACCCGTACCTACTAACCGACCCAATATCCGCAAAGACCGTGCTGACAGCGTCTTTGGTACGCCCAACATTAAGTGGCTAGCAGTGGTCAAGGAAGTCAAACGGATCCACGAAACCGGGCGACCAATCCTAATTGGGACCGCCAACATTGATGACTCGGAACTACTTCACAACTACCTCCAAGAAGCCAACATTCCCCACGAAGTTCTCAACGCCAAGAACCACTCTCGGGAAGCGGAAATAGTCGCTAAGGCGGGCCAAAAAGGGGCGGTGACCATTTCTACCAATATGGCGGGAAGAGGTACTGACATTCGTTTAGGTGAAGGGGTCGCTGAAATGGGCGGGTTGTATGTCCTTGGTACGGAACGCAACGAATCGCGCCGGATTGACAACCAACTGCGCGGTCGGGCGGGTCGGCAAGGCGATCGCGGTGAGACCAAGTTCTTTATCTCCTTGGGTGATGCCCTGTTCAAACGCTTTGCCCATGACCGGATTGAACGCGCCATTACTAAGTTAGGGAACGATACCTTTGACAGCTCCTTCTTTTCCAAGATGTTAAGCCGTACCCAAAAACGGGTCGAAGCAATTAACTTTGACACCAGAAAGAACTTAATTGATTATGACCATGTGTTGGCCAGTCAACGGGAATTGATCTACAAACAACGCGATAAGTTCTTATTAGCCACTGATCTCAGTGACATGATCGACAAGATGTTGGAAAAGTTTGTCGAGCAGTTCTGTGACCAGTACCGCAACCCTAAGAACCAAAACCTCGTTAACCACATTGCTTTATCAGAGGCCTTAAACCTGGAATTAAACATGCACGGTGTTATTAGTCCGAAGCTGTTTGAAAACATGACTTTTGATGCGACTGTCCATAAAACCCACAGTTTAATTGGTGAAAAGATTACCAATAAGGTCAAGGTCTTAACCCCCCCAATTGCCCTCATCCGTTTTCGTGAAATTATGATCACAGCAATGGACAAGCACTGAATCGAACACTTAGACAATGTCTTTAAGCTCAGGGAAGGGGTTACCTTGCGCTCGATGGAACAAACGAGTCCCCTCAACGTTTACATTCGCGAAACCGACATCTTGTTCCAAACAATGTTGCAAAAGATTGCTCGGGACGTCATTATCCAAATTGCTAACTTAGCGACTCCCGAAGAGTTTGATGAGGAACTAATGAAGGCTAACGCCTTGAAGAAGTTGCAAGCACTTAGAGAAGCTCATGAAAAGTCCAACCAAGGACAGTAAACTGTTCCACCTCAAGAGTAACTTTGCCCCCACTGGGGACCAACCAGCGGCGATTGCTAAGTTAGCTGAGTTTCAAACCAATGAACAGGTCTTACTCGGTGCTACGGGTACCGGTAAGACCTTTACGATTGCCAACGTTATTCAAAAGGTACAACTGCCTACAGTAGTGATTGCCCACAATAAAACGTTAGCGGGACAGCTTTATCAGGAGTTGAAGGAGTTATTCCCTAATAATGCGGTGGAGTACTTCATCTCCTACTTTGACTTTTACCAACCAGAGGCTTATCTGCCCGCTAAGGGGGTTTACATTGAAAAGAGTGCAACTGTTAACGAAGAGATTAAACGGTTACGAGTGTCCACCTTACATTCACTCTCTACCCGCAAGGATGTGATTGTGGTGGGTTCGGTAGCCAGTATTTACCCAACTTCTTCTCCCGCTGATTTCGCCCAGTACTCGCTCTGGTTAGTAGTTGGTAAGGAATACGGTTTAAGTGAGTTAAAAACGCAGTTAATTCACCTAAACTATGTTGTTAATAAACAACAGTTAACTCCGGGAAAGTTCCGCTTTCAGGGCGATGTTGTTGAAGTGTTTCCTGGTTATGCGCAGGATTATGTCTTGCGGCTTTCCTTCTTTGACCAGCAACTAGAACAAATTGCTCGCATCGATCCCTTAACTAACAAGGTATTGGAAACCCTTAACAGCTTTAAGTTAGGTCCCGCTGATGAGTACATAGTAAACCAAAATGACTTAGGAGTAGCCTTAGACACGATTAAGGCCGAACTCAAAGATCGCTTAAAGTACTTTGAACGGTTAAACTTCCCCGAACGTGCCCAGCGCTTACAAACGATTACCGAACACGATTTAGCAGATCTGAAGGCCTGGGGGGTATGTAGTGGCGTGGAAAACTATGCGCGGCATTTAGAACACCGCCCACCGCACTCCAAGCCGTATAACATCTTTGATTACTTTACTAAAGGTGAATGGCTATTGGTAGTTGATGAATCCCACCAAACGCTACCGCAAATTAAGGGGATGTACAACACCGATATTTCACGGAAACAAAGCCTAATTGAGTACGGTTTTCGCCTCCCTTCAGCTTTAGACAACCGTCCTTTATCCTATGAGGAATTCCGCCAGGGAATCAACAAGGTCATTTATGTATCCGCTACCCCTAGGGAAGAGGAAATTCAGTTAAGTCACAACAACGTGGTCGAACAGTTAGTGCGTCCCACCTACCTCTTGGACCCGGAAGTGATTGTGAAACCGAAGGACAACCAGGTTGAAGACCTAGTGAGTGAAATCATTAACCAAAGAAAACACAACGGTCGTACCTTTGTCACGGTGTTAACGATTAAGATGGCGGAAAACCTGACTGACTTTTTGAAGGAGCGCAACATTAAGGTAGCGTACATCCACAAGGATATTAAAGCCTTGGAACGTTTAATTCTGTTAACGGATTTACGCAAAGGTGAGTATGAGTGTTTAGTGGGGATTAACTTACTGCGAGAAGGTTTAGATGTTCCTGAAGTTTCACTCGTAGCCATCTTTGACGCTGACATTCCTGGATTACCACGCGATGAGCGCAGTTTAATCCAGATAATTGGCCGCGCTGCGCGAAACGTTCATGGCCGGGTGATTATGTATGCCAATACCATTAGCGAACAAATGGATAAGGCGATTAAGGAAACGCAACGCCGGCGCACCATCCAAATGGCTTACAACGAACAACACCACAAAACACCAATGACGGTGCAAAAACCAATTACCTTAAACCAGCCGATTAAGTTAAAGACCAAAAGCTCAGAGCAGCAAAAAGCTGCTTTAATTAAGCAGCTAACCAAGGAAATGAAGCAAGCAGCCGCTAACCAAAACTATGAGTTAGCGATTGAGATCCGCGACTCGATCTTTGAATTGGAAAAGCAATTTCGTGGTAAAATTAAGAGCTAGCAATGCGTAAACTAATTAAATTAAACGTCATTGTCTTTGTCTTGTTGTACTTGGGCGAGCTGTTTGCCAGCCTTTCGTTCAAGTTAATCAGTTGCCTCAAGACACGCAACCAGTACTCCTTAAACGGGTACTATGCGTTGTTTGTCTTTGTCAACATCATCCAAAAGATGGCTAACTCTTTCCAAAAGTTAGCTTCCTCAGTTGTGTTGTTTGAAACTGAAATTAACGAATTTTTAGTTCTCTTTACTGATACAAAGAATAAGCGTGAGGAGAGTGAACCAGTGCGCCAGGTGTCAACAACCCAAGAGTATCACCAGGTTACGCTCGACCAACAACACTACTTTAACCACAAACTGAGCGATTACTTCCGTTTGTTTAAGGACAAAACTTTCTTCTTTGAAATTATCTAGTTACTAAATTGACCTGAATTGCAACCTTTCAGGCACTTTTTACTGTTAGTAACTAGTCTTTTTTCATTTCAATATTTAAGTTTTTAATTTAACCAATTTTTACTATGAAGCTTAGTGCTATTATCTCCCTATCAGTCGCTGGTACTGTGGGAACAACTGCGGTGGTAGTACCTACAACTATAACGCTTGTAAATAAGACCCACCAAGTAGAACATGAATCAGAACAATCGGATTTTCAAGATATTCGCTTTGGTCTTAATAGTGTTAAGTTGCCAAAAGCACAGCCAGCTGCGGCAACTAGAATTACCGTGGAAAACGGGACTGATAAATTAGTCAACTATAAGTCCTCACCACAACAACTCTTTTTAGCGAAGAACGCGCTTAAGGATAAACTCCAAGGTGAGTTTGATAAATTCCTAAGTGATGCGAAGGCCTTCCCAGCGCTAACCGCTGATTTACAGGAATGGGTTGACCAACAGCTGTTTAATCCAAACCAAAGTTTCTTTGATTTAAGTGCGCCCAGGTCAAACTTTACCCTTTCATCTGACAAAAAGGCTAGTTTAGACTTTATCTTCCGCTTTACTAACTTCACCGAATCCGTTCAGTTGTTAAAACTACCAGAAGGTGTATCGGTTGTAGTTGACTCCAAACAAAGCTTTGATTACTATGTCAATGCTAGTGCCCAAAAATTATTAGTTCTACCGCTGTCTTTACCAGATTACACTTTGGGTTTAAACTATATGTTTGACCACATTACTTTAAACGGTAAGGTTGTCAATAAATTTAGTTTTAATCCGTTCAAAACGAATTTAAACCTCGCCTTTAGCAACGTTTACAATGGCGTTGATGTGTTTGAAGCACAAAAGAATTTAGTAGGTAAGGGTAAATACCTCAACACCCACGTGAAGGCTGAAGACGTAAAGAAGGACGTTAATGCCAACATTAAAAACCAATTTGACATTGCCAAAATTATCGCTGAGCTAATGGGTAAAGCCCTTAAAGAATTTGGCAATCAACAAGAAGGTCAACCATTATCCTTCCTAAAGGTAATGGATAAAGTTAAAGAAGATTTTGAAAAACTGTTTAACTTAGTCCGTCCTGGATTGGGTAAATTTGTTAAGGACTTAATCCAAAGTAGTAGTCAAGCAGAAAACAAGATAACTGTCTACAAGTTAATCTTTGACAACAAAAAGACCATCTTAAACCTACTTAAAGAGCTTTCCATTCCGGAATTAAACTCTTCTTTAGGTTTAGTGGACGTCTTGTTTGATGTCATTACTGACTCTGATGGTCTCTATGAAAGGTTGCAATCTTTCAAAGACTTAATCGTTCCAGCAGTTAAAACGAATGAAAAAACCGCGGCTTTAAGTCCATTAATTGAAGAGTTATTAACCCAAAAGGATACCTATGTGTTTGACTTAATTCAAAAACACAAGGGTATCTTGACTAACTTGTTAAAGAACTTCTTAGCTGATTTCCAAAAATCAACGCCGTTTATGGCTGATCAAGTAGCCATCTTCACTGAGTTATTTGACAACGAAGGTGCGTTTGATTTATTTGGTGAGGCTGACTTTGTTGACAAGATTGCCGAACTCTTCTTAACAAAGCGTACTGTTAAAAATGGTGAAAAAATTGAAACTAAAGATTCCCTACTGGTAACATCATTAAAGAGTCTTTTAGGGGAAAAGGTAGCTGCCTTAGATGATTTGTTAGATAGCTACATCTTTAAAAATGAATTACTTAACCGCAGTGTAGAAGTGGCTAAGGCTGAAGCTAAGGACACTAAAGGTGCTACCGATTACAAAAAGGAACAAGCTAAGGCGCTCAAAAAACTCTTTAAACACATTGGTGAAAACACTTTAAGTAAAACCAATCTCGATAAAATCACCTTAAAAGAAGTTAAAAACACCGAAAATGTTGAATTAGAAGAAACTGAAACAACCTTAAAGGTTAAAAAACTCGATGTTGAATACAAGGTAGAACTTGGCAACTTTGAAATCAAGAATGGCTTGATTAAGGCAATGCTCGAATTCTTGCCAGACCCTAAAGATTTAGAAACAACTTTAGATAAACTCTTGTTCAAAGGGGAAAGCTACAAAGCGATGAAAGACAAGTACATCAAGGAAGGTTTCCCTGGTTATGGTTGGGCTAAAGGAGTAGTCCCTGGGGCCTTTGAATCTATTGAAAATACTTTTAAGAGTGCTATTGATAAAACCAAGTCCATTCGTGATCTCTTTGGCGACATGCTCTTTGGTAACGATTTAAGCAGCGTTAAAGAAACTGATTCATTCATCACCCTAGGTGGTTCGTTCGACATTAAGTATGGCGGTGAAAATCTCAATGTGTTGCCAGCTTACTACTCTTTAATTAACAGCGAAATTGGCTATCAAATTATTGGTGTAGATACCACAATTGATGCAACTAAGGTAAAAGTTGAATTAAAAAACAAAGAGTATAAGGGTAAATCGCCAGCTATTAACGGTCAGGTGAAGTTGTCACAATCATTCTTTAATGTTTGGACAAATATGTTTGACAGCATTACCAAACAAATCTTCCAAAAGAAATACGAGTTCAAAGATAACATCCAAGTGTTTGCGCGAAACGAAGATAACACATCACGTTTAGAACTTGACATTTCTGATCCTGAACAACGGGTAATTCCATTTGCTTTTGTTGATGGCTTTGGCATTCAACTCAAAGCAGTTGACAAAAACATTACGAAAGAAGCAGGTAATACTGAGCCAAAATCTCCTGTAATTCAACTTTATGAGGCACTTAATAAAGAAAAAGATCAAAAACAACAAAGTAAACAATCTCCAAAACAACTTGATACTAAAACACAGTTGGGTTACCTATTGAAATTAGGCGACAATTGGAGTAAAGATGATTACAAAAGCTTAATTGATGATACGATCATCAATAACAACTATTTAGAGGCCAGCTTTAATTCCAAGATAACGGTTGATCGCTTGGGTATTCCTATTGACCTTTGATTGTTTAAGATTTGACCTAAGTTCAATTTGGAAATCCCAATGCAAGGTTCCTTACAACTTTACAGTAGTAGTGTTATCTTCCCATACGGTATTTATGACACCAGTGTTCAAGATGCTACGAAGATTGTGAAGCGTCTGAACTTTACTGACATGGGCTTCAAACTCAACGATCCAAAACCTAACTTCTGGTTCGTTGGTTTTTAGAGCAGACTAACTACTATACTGATTAAGCTACCTAGAAGGTAAAGCGAGAAGTTCATCCACCAAAACCCGCGGGTTTGTTTACGGTAGGATGAACTTTTTGCTTTATACTCGTCAAAACTAATGTTGGGCTTGGCGTATATACTACCTAGTTTTAGATATTGGTTAAACTTGCTAATTTCGTACCGTGCTCCATCACAGGTCTTTAAGCGCACTATACCTTGGAGACACACAAGCAATCAAACAACGACAAAACTAACAACACAACTGTTTTTCAGAATTAGGTTAATGTTGTTAGTTTTATTGAAAAAAACGGTTAAATTCAAAATGGCGAAGAAAAGCACAATGTAAATTGTGCTTACTATTAACCCGCCGTATTCACGCGGCTTCGAATTTGTGGTTTGTGTACCTAACATGTACTTACTAGTATCGTTTTTTTGTAGAATGTTTTGAACCACTAACAATGTTTTTAGCGACTAAAATGCTGTTTGACTCCGAGTGGAAACCGATTATAAATCGCGTTTCCCTGTAGCCATCTGCACTTTTAGTTGGACTGGTTACATCAAATCTTTCAGGTAACCAGAATATTATCATGCTGAGGGACCTGAGAACCAGGTTCCTTTTTCTTTTTTATTAGTATTCGAAAGTGTTCAAGTATATTATTAAACGTTTGGGTTTGGCAGTGTTAGCGATGTTTATCGTGATGACCATTGTCTTCTTTTTGGTTAACTCCACAGGTCAAACGCCCTTATCAGCAACCTCTTCTAAGGATTTGGAGGCTGTCAAAACACAACTAGATGCCTTTGGTTTTAACGATCCGTTAATTGTGCGATATGGTCGTTACTGACAAACCCTCTTTTCAGGTAGTTTGGGTACTTATTACAGCTCCCCGAACCAAACAATTGACCAAATTGTCTTTGGTCGGGTACCTAACACCCTTTACGTTGTTTTAATTTCTTTCTTTATCGGCTCTTTGTTGGGCATTATCTTCGGGATGATTTCCGGATTATTCCGTGGCAAGTTAATTGATGCGGTAATTAACGTGCTCGTTGTTTTATTTGTTTCGATTCCTTCCTTTGTGGTTGGTTTAGGTTTACTTAAGGCAGCTGGTTTGTTCCGTTTACCACCGCGCTTTATTAACTTCGATGATGCTAACTTTAACTTTGGTAACTTCTTGTTAGCCTCCATTATTCCTATTTTATCCTTGGTATTTTACACCTCAGCGGCCTTTACTTATCGGGTACGGAATGAGGTCGTGGAAGTAATGAACCAGGACTACATAAAAACAGCACGTAGTAAAGGTTTGAGTACTTTTGCTGTAGCGCTTTACCACATCTTCCGTAACTCAATTATTCCTTCTGTACCTTTATTTGTCTTTGGGATCTCTGGTGCCTTTTCGGGTGGTTTTATTATTGAATCCTTATTTGGTGTTCAGGGGGTATCACGGATCTTAATTGACTCGGTGCAAAGTAACGAAACTAACCTCGTGATGTTTAACATTATGTTCATCCAGGGGATTCCCTTACTAGCAAGTGTCTTCATTGAGTTGATTTATGTCCTAGTGGATCCTAGGATTCGGATTGCTAGTGCCGGTGGGGTGAGTCTGTGAACTAAGCTTAAGTTTGTTTATTTACGCCAAGCTTGATTGCGTAAGTGAAGACGGATTAACCACACTAATTCCCACAATGTGTTGTTCAATTCGCCCCAACACCGCCAGCTGTTAGAGCTCAAAGCAATTGATTACAAGCACAATACCATTAGCTTAACTGAACAGCAAAAAACCACCCTTAAAATTGAACCAACTGCTAACTTTGTTTTGTTAGGAACTAAATGTTTAAAGATTATTACGATCCATGGATAAGCACCAAAAATTTGACCAAAGCCTGTTTCAACGGGTAGACATTAATGTCTTAAAACGCTCTGACCAGTTGATTGGCAAACCAACCACTAACTTTGTCGAAATTATGAAGCGGTTGTTCCAAAATAAGTGAGCAATCCTCTTCTTTTTACTGATTGTGCTAATTATTTTGTTAGCCATCATTGTACCCTTAGCATCACCTTATTCAGCCGTTACTCCAGTATCGAACAACGCTTTGGCACAGAACCTACCACCACGATACCTCTGGAACGGCGCGGGTGACATTCAAGTAGAGAAAATTACGGCACGCTCGATTGCTGAAGTGGCCCAATCAAGTGGTGTTTTGGTAGGGAAATTACCGGAGGCAAGTAGCAATCCACTCGCTACTAACGTTAAGTACAACATTGCGCCTTACCAACTAGCAGAGCTGAAAAACTACTATCCATTATTAGGCACTAACGGTTTAGGGGTTGATATTTGAACTTTACTGTGAGCCAGTATGGCCAAGTCACTCTGAATTGCGATTGTGGTAGCCTTAGTTTCAATGGTCTTCGGCACCATTTACGGCGCGATTGCTGGGAGCTTTGTTGGACGCGCTGCTGACAACATTATGAGCCGGATCATTGAAATTATTGATTTGGTTCCTTCAATTCTCTGGATCATTGTCTTGGGAGCAACATTCCGCTTTGGTGGGGTAAAACAATTTGATGACAGTGTGGTGATCTTTACCTTAATCTTTGTCTTTTGGACCTGACCCGCTGCTACCACAAGGATCTACATCTTAAAGAATAAGGATACGGAGTACATCCAAGCAGCGCGTACCTTAGGAGCAAAACAAATTCGCATTATTTTTGTCCACATGCTACCAGTTGTAACTGGTCGTTTAGCAGTCGTCTTTGTGAGTTTGATTCCCGCTGTGATTGGTTATGAAGCATCACTGGTTTTCTTAGGGCTTAAACCTGCTACCGAAGTGGGACTTGGTGCTTTATTAAACCAGGTCACCAGTAGTGGTAACATAGCACTGATTACCAGCTCAATTGTGAGCTTTGCAATTCTCACCGTTTCGACCCGCGTGTTTGCTAACGCTTTAAATGACGCAATTGACCCACGGGTAATAAGGAGGTAATATGGCACTAAAAGCAACTAATTTTTTTACAGAACCAGAGTACAAACTGCAGGATGATTTGATCTTAGACATCCGTGATCTTCACGTGAGTTTTAAGGTTAAAGACGGCATTATGCAAGCTGTCCGTGGTGTTGATCTCAAAGTCAAAAAGGGCGCCATTGTCGGCATTGTGGGCGAATCTGGTAGTGGTAAATCGGTCTGTGTTAAGTCGATTATTGGCTTTAACGATGGCGCAAAAACGACCGCTAAGTTAATGAACTTTAAGAACATTGACATTAGCAAGATTAAAAAGCACCAGTGACAGTACTACCGTGGCACGTACGTGTCTTACATTTCCCAAGATCCACTGTTCTCGTTAAACCCCACCATGACCATCGGTCGCCAGGTTAAAGAAGCGATTTATGTGTCGTGTAAGCGCCGATATTACCAAACTAAGAGTGACCTCAAATACGATTTGCAAACAGAACGAATTGACCTGGACACTTACAAGGAAAAACTAGCACAAGCCAAGGAAACTTACAAAGCTAAAACGACTAAGGCGGCGGTCCATGCAAAAACGCTAGAAATCCTTAACTTCATTGGCATTGACCAAGCTGAAAAGCGCTTAAAGGCCTTTCCAAGTGAGTTTTCCGGGGGAATGCGGCAACGGATTGTGATCGCGATTGCCGTAGCCACCGAACCGGATTTAATTATTGCCGATGAACCGACCACGGCGTTGGATGTGACCATCCAAGCGAAGGCGTTGAACCTAATTAAACAGCTGCGTGACCTGCTCAACATTACCATTATTTTTATTAGTCACAACATCTCGTTAATTGCTAATTTCTGTGACTTTGTATACGTAATGTACGCTGGTCGATTAGTGGAAAAGGGCTTAGTTGAGGAAATCTTTACCAATCCGGTGCACCCCTACACTTGGGCATTAATGGCTTCTATCCCTGAAGGTACGGACAAAAACGCTCCGCTAACCTCAATTCCTGGGTATATTCCCAATATGTTATCCCCGCCTAAGGGCGATGCCTTTGCAGCGCGGAACCAGTTTGCACTTGCCATTGATTTCGAGCACCAACCGCCGTTCTTTGATGTAACGGAAACTCATAAGGCCGCTACCTGGCTACTCCATCCCCAAGCACCGAAGGTAGAGATGCCCGCCGATGTCAAGGAAAAGATTGCCATTACCCGCAAAGCACTCGCCATTAAAATGCCATCTCAACCTGTAAAACAACCAAAAAGCAATGGAAACAAAAAAACAAAAACAAAATCCGCTCGTTAATGTTAAAGCCTTGAGCATGTTGTTCAAGGTCCGCGGTAGCTTCTTTAAAGCCTTAGATGAAATTGACTTTACCGTCAATGAAGGTGACTTCTTTGGGGTCATTGGCGAATCGGGTAGTGGTAAGTCAACCACGGGGAAGTGTTTAATCCGGTTAAATATCCCGAGTGGCGGTAAGGTGGAAATTGCTAACCACCTTATTTCTGGTAAAAAACTAACGCGCGAAAACGACCACTGGTTAAAGCAAAACGTGCAGATGGTCTTTCAAGACCCATACTCTTCTTTAAACCCTACCAAGAATGTTTTAACGGTCATTTCCGAACCGTTAGTAATTACCAAAACGGTGTATGGTGAGGTAAAGGAATATCTTAAAACGCTCGCTAAACTGTCCTTTAAAACCAAGAAGGAATTGTTAAGGGAAGACTTTGAACTGGAAACCCAGTTTTACGAAAAGTTCTTTTCAAAGGTACTGTTTCACTTAGAAACTACGATTAACAAGTTTGCCCTACTCCAAGAGAGTAATAACAATAGTTCAGCCGAGTTAGCACAAACCATTTTGGGTCACACTGATGATCTAATTGAAGCATTACGGCAAGAGTTCGGTCTGGTCTATGAGTTTTCCTCATCCCAAAGCGAACCGCTACAAAAGGCCCTCAAAGACAAACAGGAAACCTTAGCACAGGACACGATTGACAAGTTAAAGCAAGAGCTGTATACAACGCAGCAAAAAGCTAAAGTTTCCACCCAGGCCTTTGCTACTTGACAAAAGTTGCAACAAACTAAGCAGAACTTAAAAGCGTACCGTGCCCAAATGGCGGAAGAGTTGCAAAACAAACCACGGATTTACCTCAATGCATGGTTGTTAACAACGAAGAACTACATTAAGGATTCACGCCAAAACACGCAGTTAACTGACGATGTCTTTGCTTTTTCTTACAACGACATGGTCGACAAAAAGCGCCGCTTAGTGTTAGTGTTATCGGAGTATTACAAGGCGTTGCCGTTCTTTTATGACAATTGGATCCACCAGAACGCCGATCGTTTTGATGAATTGACCAACGCAGTCTTCTTTGACTTGATTGATGTGGTGATTGCCTTAAACCGTGATTTTGCTAATGTGGAGAGTGACGCTAAGGCCGAGTTAATCCGTTTTGTTCAGTTTATCCGTCGCTTGTGTGACTTACGCTTTGCTGCTTTGAAAAAGAGTTTTAAGAAGCAAACTAACTATAGTTTTGACTTTAACCGCGAAACCGAGTTGTTATACGCCAACAGCTGTTATGACATTAAGGAGCTCCCCCAAGTAATCCAACCATACTGGGAAAAGCTCTTTAGTGATGCTAACTATGACAAGATTGCCAAATCAGTGCAGGAACTCAACGATATTATTAGCACTGACATTGAAAAGGCATCGAATATAGCCAGTGAAATTAATACCAAGATTAGTAGTTTTAAAACTGAAATTGCGGAGTTAAAAGCTACCTTTAAAACAGAAAAAAAAGCAGAAGATCACAGTGCCCAAATTACTGGCTTAAAAACGCAAATTGCTGAAATTCAAACGCAAATCAAGCAGCAAAAACGCGAAGTCCAAAGTACCGAAAAGGCCGCTTTAAAACCAGTATTAAAGCAGTACAAGAGTGCGCTGCACCTCTATAAACGCTTTAAACAGTTGTTACGCCAATTTACCAAGCAGTTAAACCTCCTTGTCAAAAAACAGCAGGAATTAGAAAAGATTGAGGAAGGCTTGGATCTAACCATTTGAGAGCGGATCCAACTGTTGTTCCACCCGGTCGAAGGTGATCTCAAGAGTGAACTCAAAACACGCTTAAAGTCGTTTGGGGTGATTAACTTTGAGTACAAACGGGCTGTGCGTGAATCCCGTGTCTTTCGCTTAGTCCACTTTGGTCATGACGTGATGAAGTGAGGATTGTTCTTACCATTGACCAAGATCTTCATGCGTAACAAGGTGTATGAAGCATTAGACAGTGTGGGCCTCAAACGCGAGCACGCTTATCGCTATCCGCACGAGTTTTCTGGGGGTCAGCGGCAACGGATTGCGATTGCCCGCGCTTTAATTACCAAACCTAAGTTAATTATTGCAGATGAGCTTATTAGTGCTTTGGATGTATCCATTCAAGCGCAGGTGATTAACATCTTGAAGGACTTGGCTAAAAAACACAACTTAACGGTGCTCTTTATTGCCCACGACCTTTCGATGGTACAAACAGTGTGCAACCGTTTAATTATTATGCACCGCGGCAAAATTGTGGAACGGGGTAGTACGGATGAAATCTTCGCGCACCCGGTCCACCCTTACACCCGTTCCTTGATTAAGGCATCGCCAAAGTTGAGTAAGATTAACATTGACCTAGCCTCCTTTGACGAGAAGTTTACGTATGACAGTGATTATTCACTCACGAATATGCCAAGCTTCTTGAAGGTGCCAAATACCCAAGAACACGAGTTGTACTGTACTCAAGGCCAGTTTGACAGTTGGATCAAAGGAGCAAGTCGGATAAATTAATTTAAAATTTCTAGCAAATTGTGGGAGCTAGTTTAGCGTTATTACCACACCAATTATGGCAAAAAAGACTATTACAAGAATCGCTAAGATTAACCTCTTAGGCGGGCAAGCAAAGCCCGGTCCCGCACTCGCATCTGTTGGGATTAACATGGGTGAATTCACCAAACAATTTAACGAAAAAACCAAGGACAAACAGGGCGAAATGATCCCCTGTGTGATCACCGCTTACAACGATAAGTCGTTCGACTTTATCTTAAAAACTACCCCAGTTAGCATCTTGCTTAAGCAGGCTGCAAAACTGGAAAAGGGGGCGAAAAACGCCAAAACAATTGTGGGTAAGATTACGATGGCCAAGGCCAAGGAAATTGCGCAGTACAAGTTAGTGGATCTGAACGCTAACACCGTGGAAGCAGCCCTTAAAATGGTTTTAGGTACTGCTAAACAAATGGGAATCGAGGTGATCGAATAATGGCTAAACTATCAAAGAAAATGAAGATAGCTGTTGGTTTGGTCGATAAAACCAAGCTGTACCCGCTCCAAGAAGCAGTTGATTTAGTCAAAAAAACTTCAATTACCAAATTTAATGGCTCAGTTGATATTGCCGTTAGCCTTAATTTAGACACCACCAAGGCGGAACAACAGCTCCGCGGTGCGATTGCTTTTCCTCACAGTGTTGGCAAACCAATCCGTATTCTAGCGATCACCGATGATGAAAAGGCGGCACTGGAAGCAGGTGCTGACTTTGTTGGTGGCATTGACAAGATCAATGACATTAAAAACGGTTGACTAGACTTTGACCTGATCATCACTTCTCCAAAGTTTATGGCCGCATTAGGTAAACTCGGGAAGCTGTTAGGTACCAAGGGTTTAATGCCTAACCCAAAAACAGAAACCGTTACTGATGATGTTCCAGCTGCTGTACGCGCTTACAAGAAGGGGAAAAAGGAATACCGCGCTGACAGCTTTGGTAACATCCACATGTCCTTAGGCCGCGTGGACAGTGCGTCCAACCACTTGGTTGAAAACGCTTTGGCACTGCTTGATCTCATTAAATCACGTAAACCAGCAACCGTTAAAGGAATTTACATTAAGAACATTGCCTTGACCACTACCATGGGTCCGAGCTTAAAGGTTAAGCTCCCTGATTAATGGACAAGTTGCGCCTCGTTGTTGGTTTAGGTAATTTAGGTAAGCAGTACGCTGAAACCCGTCATAACGCGGGGTTTAAGGTAATTGATCGTTTACTAAGCCTTTACCATGTCCAGTTAGAGGAACGCAACAACTTAGGTGAATTCATCCTTTTAAGAAAACATAAGGTAGTGTTAGCTAAGCCAAACACTTACATGAACCACAGTGGTAAGTTTGTTAAATGAGCTTGTCAAAACTGAAACATTAAACCAGATAAGGTAATGGTGGTTTATGATGAATTAGCCTTCCCGTTAGGTACTGTTCGACTAAAAATGCAGGGTTCAGCCAACAATCATAATGGCATAAAGTCAGTAATTGCGCATTTAAATACTGAACACTTCAACCGCCTCCGGTTCGGAATTAAGTCCGATAATACTAGTAACATTCTCCATGAAGTGGTAATGTCGGAGTTTACCCCAGCCGAGCGTAATTTACTTGAAACAGCGCTTACAAAAGCGATTGAAGCACTCAAGGGTTACATTGATGGTGTGACAATGCTTAAATTAATGGAAGTTTTCAATGCCTAAAACACAATACATAGCTGGAGTATCAGGTGGTCCTGATTCAATGTTGTTGTTAAAACTGTACCACAAGAAGATTGCCTGTGTTGTACACGTTAACTACAATAAGCGGACTACTGCACTCCGCGACCAAAAAATGGTCGAAGAATACTGTAAACAGCTCAAAGTGCCCCTCATTGTGCACACGGTACCTGAAGATACCGTTTGAAAGAAAAACTTTCAAGCACAAGCTAGAAAAATTCGCTTTGAACAGTTTCAAAAAGCAGCTAGCCTTTATAAAGTAGATAAGCTGCTGTTAGCCCACCACCGCGATGATTTTATCGAACAGGCCAAAATGCAGTTGGACGCACGCAAGCGTGCCATGTATTATGGCATTAAAACACGTGGCGAATTGTATGGGATGAAGGTCTATCGTCCCTTTATTAAGTACTGAAAAAACGAGATCTTAGCCTTGTGTGAAGAACACAAAGTGCCTTATGGTATTGATGAAACCAACGCGCAGCCAATTTACAAACGTAATCAGGTACGCCAGGAGATCGCTAACTGATCGAAGGAAGAGAAGGAGGAGTTTTACATTGGTGTGTGCGCCATGAACCGCGTCATTGGTCAAAAGCTCTTTTCCTTAATGAAACGTGCAAAACAGTGGTTAGCTCATCCTGATGTACGTGAATTAAAGCGTTACCCACTTCCCGATCAACGTCAGTTGGTTTACAGCTTTTTAATTACCCACCACATTGATGTAACGGGGGACAAGATTGATGCCATTTTAGACTTTATCCAACCGTTTCAACAAAAGCATTACCGTCTCAAGGATGAGATCTTCTTATCCATTAAAGACGAACGGCTAACTTTACTGTACAAGAGTTAGATGAAAAAGTTATTAGTCAAGGAGTTAATCGAACAATTTCAGGATTGTGTTAACCTCATTGATGGGCACACTAACACCAGTAATGTAATTAGAGTACCCGGATTAAAGCGGGTGGTGTTTGAAATGCTGGGGTTGTTTTCCAGCCAAATTGGCTCGGTAGCCATTTTGGGCAAACGCGAATTTGGCTTTTTGAGTCAAAAAACGCTGGTTGAACAACAGCAAATTCTCCACAACTTACTCAAACTCAACCCACCAGCGATTATTTTGACCAAGTCCTTTACTGACCCCACGGTACTTTTACAGGTTAACCAGACTTATCAGGTACCGATCCTAAAGACCGATTTCTTTTCCACGGAACTTTCCTTTACGGTCGAAACCTACATTAACGAACAGTTTGCTACTGTTGCACAAATTCACGGCGTCTTGCTGGAAGTTTTTGGTGTAGGTGTCTTATTAACTGGTCGCAGTGGCATTGGTAAATCGGAGTGTGCCTTAGATCTAATTAACAAGAACCACCTCTTTGTCGGTGATGATGCGATTGAAATATACCGTTTGGGTAATCGCTTGTTTGGTCGCGCGCAGGAAGTAGCGAAAAAGTTTATGGAAATCCGTGGTCTTGGTATTATTAACGTCGAGCGCTTTTACGGATTGCAAATTACTAAACAGCGCACCGAAATCCAGTTAATAGTGAACTTACTGAGTTTAGAAAAACAAACGACCGTGACCTTTGAGCGTTTAGGTACCGAATTGAAAAAACAACGTTTACTCGGAGTTGATTTGAGCTTTTATGAAATTCCGATTTCCCCCGGGCGGAAGACGAGTGAAATCATTGAAAGCGCTGTAATTGATTTTAAACTTAAGCACAGTGGCTATAACTCTGCTTTAGACTTTATTGAAAACCAGAAGGCCATTTTAAAACGTAAAAAAGATGAATCCTAGTGTTAGTAGCAGACCACCATGATCAACGGCCTTTTACTTAGGGCCGGGTTTTCCTATTCAATGGTATGGGATCATAGTCGCAATTGGGATTGCCTTTGGCATCTTAATGTTTGTCTTGAAGTTAATTTACTTTTACAAGATTCAAGATAACAGCTTTTACTTCTTTATCTTCATTGCAGTTTTAACAATGGTATTGGGCGCACGAGCTTGGTATTTTTTGATTGAAGCGGTTGATGGTCGTTCAAGTGGGAGCAACTTCTTTGACTTCCGCAACGGTGGGTTAGCAATCCAAGGTGGTGTGTTGTTAACTACGTTAGCGGGGATTATCTACTTTAACGTCTTTTTAAACATGAAGACGACCAAAACCAAAACCACCGCTAAACTATTAAACAACAAGAACCAGATTAAAACGGTTTATGTCGAGCGTAACATCTCAGTGTTTGTGATGTTGGATCTCATCGCTCCCTGTGTGTTAATTGGTCAAGCCATTGGTCGTTGGGGTAACTTCTTTAACGCGGAAGTTTATGGCGCAGCTTTAGTGGGTTCCAAAAATGATACCCTGTCAGCTGCTAACACCACCTGGGGCTTTTTAAGAATCTTAATGCCCAAAGTGTGGGACGGAATGTTTATCAATGGTAGCTTCCGCATTCCGCTTTTCTTAATCGAATCGTTCTTTAATACGATCTTCTTTGTCTTTATCTACTTTGTAATGGACCACATTAAGGGGATCCGCAGTGGGACAATTGGCTTTAGTTACTTCTTAGCGACTGGCATAGTTCGCTTAATCTTGGAAACGCAGCGTGATGAAGCGTTTAAGTACAATACCTCGATTGTGTTTAGTGCACTCTTAATTTTGGTGGGAATAGTAGGGATTATTTACTGTCAAACGTTAGCAATTAAATTGCGTGGTTATTTCTGAACTTACTTCTTCCTATACGGTTGGTACAAGGTAGCCGCTTTCTTTACTACCTTATTTATGAAGGATAGGACACAGGCTTGTTCTAGTAAGTTTGCATTTTACGAAAAGAGCTTACCTGAAAAGGAACGTTCCTTCTTTCAGTTGAAGTACTACAACGATGTTTTACCACCGAAAATTTACCGTTTGTACGACCATGAAATGCTCATGTTTGACAAGCTAGAAGCAGTGCCGGAAGCTTAGATTGTGTTACAATATTCGCGCTGTCTTTATGGCTACAATCGCACAATTAATTAGAAAACCGCGGAAAAAGAAGAAGGTTAAATCTAAATCACCCGCTTTACATTACAACCTCAATTTACTCAACAAAAAGGTAACCAACGTGTATTCACCACTTAAACGTGGTGTGTGTACCCGGGTTGGTACCATGACTCCAAAAAAACCGAACTCTGCTTTGCGCAAATATGCCAAGGTAAGACTTACTAACGGTTTTGAGGTGTTGGCTTATATCCCTGGTGAAGGACACAACCTCCAAGAACACAGTGTAACGCTACTCCGTGGCGGTCGGGTGAAAGACCTCCCTGGTGTGCGTTACCACATTGTTCGTGGTACCTTAGACACTGTTGGTGTGGAAAAACGCCGTCAACAACGTTCAGCTTACGGGGCTAAGAAACCAAAAGCCAAGTCCTAATATCTTAAATTCCTTTTTTTACGAAAAACATTAACTCAATCAATTAACTTTATATGCGAAAAAATCGTGCTCCCAAACGGACTGTTTTACCCGATCCCGTTTTTAACAATACGCTGGTAACCCGGATTATTAACGTCATCATGGAAGATGGCAAGAAGGGTTTAGCACAACGAATCTTATACGGTGCTTTTGATCTCATTGAACAACGCACTAAAGAAAAACCCTTAACCGTGTTTGAACGCGCTGTGGACAACGTGATGCCGCGCTTGGAACTCCGGGTACGGCGGATTGCGGGATCCAACTATCAGGTACCTACCGAAGTACCTCAAGACCGCAAGATTGCTCTAGCACTCCGTTGAATTGCAATGTTTGCGCGTAAGCGTCATGAAAAAACGATGTTAGAGAAGATTGCTAACGAAATTATTGATGCATCGAATAATACTGGTGCAGCCATTAAAAAGAAGGACGATACGCATAAGATGGCTGAAGCCAACAAGGCCTTTGCCCACATGCGTTGGTAATAGAGAATAGAGTTAGTTATTTATGGCGCGTACAGTAGATTTAATTAACTTCCGTAATTTCGGAATTATGGCCCACATTGATGCGGGTAAAACCACCACCTCAGAGCGAATTTTATTTCACTCAGGCCGAATCCACAAGATTGGGGAAACCCATGACGGGGAGTCCGTGATGGACTGGATGGAGCAGGAAAAGGAACGCGGTATTACGATTACCTCCGCAGCTACTTCAGTTAGTTGGAAGAACTGCAGCTTAAACCTAATTGACACCCCCGGTCACGTTGACTTTACCGTAGAAGTAGAACGCAGTCTCCGTGTCCTTGATGGTGCCATTGCTGTGTTAGATGCTCAAATGGGGGTAGAACCACAAACCGAAACGGTGTGACGCCAAGCATCGCGTTACGAAGTGCCCCGGATTATCTTTGTCAATAAGATGGACAAAACGGGGGCTAACTTCGAGCGTTCTGTACAATCGATTCAACAGCGCTTAGGAGTAAAGGCGGTACCTATCCAATTACCAATTGGTGCGGAAAACGACTTTAACGGCATTATTGACCTGATTGAAGAGAAAGTTTACTTCTTTGATGGTGGTAAGGAAGAAAAGGCCGAGGAAAAACCAATTCCCGACCAGTTTAAGGATCAGGTCAAACAAATGCGTGCGCACCTAGTGGAAGAGGTGGCTAACTTTGACGATCAGTTAATGGCTGATTACCTTGAAGGTAAGGAAATTAGTATTGCCGACATCAAGCGCTGTATCCGCAAAGGGGTAATAGGTTGTCAGTTCTTCCCGGTGCTTTGTGGTTCTGCCTTTAAAAACAAGGGGATCAAACTGTTATTGGATGCTGTAGTAGATTTCTTACCTTCCCCAGTTGACGTACCGCAAGCCAAGGCTTATGGTGAAGATGGCAATGAGGTGTTAATCTCGGCTAGTGATGATGCACCGTTTGTTGGTTTAGCCTTTAAGGTAGCGACTGACCCATTTGTGGGACGTTTAACCTTTGTCCGCGTTTACTCCGGAGTACTTAAGTCCGGTTCTTACGTTAAGAATGTAAGAAAGAACAAAAAGGAACGGGTATCACGTTTGGTAAAAATGCACGCGCAGAACCGCAATGAAATCGAGGAAATTCGTGCTGGTGATATCTGTGCTATTATTGGATTAAAGGACACCACTACTGGAGAAACCCTAGTTGATGACAAGATTGATGTCCAACTAGAAGCGATGCAGTTCGCACAACCCGTGATCTCTTTAGCGGTGGAACCAAAAACCAAGGCAGATCAGGAAAAAATGTCGATTGCACTCTCGAAGTTAGCGGAAGAAGATCCGACCTTTAAGACCTTCACTGATCCCGAAACTGGGCAAACGATTATTGCGGGAATGGGTGAATTGCACCTAGACATCCTAGTGGATCGGATGCGCCGCGAGTTTAAGGTTGAAGTTAATGTGGGCGCACCACAAGTAAGCTTCCGTGAAACCTTCAATAAAGAAAGTGAAGTTGAGGGTAAATACATTAAACAATCGGGTGGCCGCGGTCAGTATGGTCATGTCAAGATTCGCTTTGAACCAAACAAGGACAAGGGCTTTGAGTTTGTCGACAAGATTGTTGGTGGTCGGATTCCGCGTGAGTACATTAAACCAGTGCAAGCGGGTTTGGAAAACGCTATGGCATCAGGTCCGCTAGCTGGTTATCCGATGATTGACATCAAAGCTACCTTGTTTGATGGTTCGTTCCACGAAGTTGATTCGAGTGAAATGGCCTTCAAGATTGCGGCATCCTTAGCTTTAAAAGAGGCGGGCAAGGTATGTAGTCCGGTACTGTTAGAGCCAATTATGGCAATTGAAGTGACGGTGCCAGAACAATACTTTGGGGACACCATGGGTGACATATCATCGAGAAGAGGTCTCATTGAGGGGACTGAACAACGTGATAACGTGCAGGTAATTAAGGCCAAAGTACCACTCAAAGAGATGTTTGGTTACGCCACTGATTTACGTTCCTTCTCTCAAGGTCGGGGTAACTATGTGATGCAGTTTAGCCATTACGCTGAAACCCCTAAGAGTGTGGTGAATGAGATAATTGCCACCAAGAAATAGCAAAATAGATAGGCATAAATCTTTACTTTTATATTTAGTTTTCCCATAAAGTTACGATGCAATACAACATTATTCTTTTAGTTGATGGTTCGCTCTCGTTAGAGCAAGCTAACCAAGTGAATGAAAAACAACAGCAAACGTTAACCAACGTTGAGGGTTTGCAAACAGAATACCTTGGTTTAAAGGAGTTGGCATACCCAATTAAGAAACAACTCTCAGCGCATTATTACCGATGGAAGTTTAGTGGTGATAACCAATCCACGAAGGACTTTAAGCGTACCGCTAACATTAACAAGCAAGTATTACGTGAACTGATTATTAACTTAGAACGTGAGTACGGCTACTTAGCTTCGATAAATCCAAAAAAGCAACAGCTGGCACTGCAAAAACGCGCTAAGTATGACGAAATCATTGCGCGGGAAAACAACCCCGAAAACCCGGATGTACCAGTAACATCAGGTTTAGCTTCCACCCAACCACGCTTATCACGTACTGAAAAGGCACAAAAACCAAAGGAAGAGCTGTGGGATGTGGTGCAAAAGATGGGAAACTTTGACAGCGTTCAAGCCAACCCATACCGCCCACGTTTCAAACGCTTTAACGCGGAACACGTTAACCAACGGCAAAACCAACAAAACAATAACAATAACCGTTTTGACCGCAACCGCAACCGTCAGCACAACCGCTTTAAGGATAAGCAATAATGAACCGCGTTTTTTTATTTGGTAAGCTCTCGTTTGATCCTAATAAATTGCAAACCCGTACCAACAATATTGGCGCGTCCTTTTCCTTAGCGTGCATTGATTCGAGCGGTTTTAATGACAGTAAGTCCTATATTAGGATTACTGCTTGGGGTAAAGTTGCTAGCTTTGTTTTAACTTTAAAACCCGGTGACAGTGTTTTTGTGGAGGGGAGATTGTCCACTTACAAGATGAACAACCGCAGTGATGATCCCAATTCGAAGGCCACTTACGCGCTCCAAGTTATCGCTGATAAGGTTTACCGTCCCGATGAGGAAAACAGTTTAGAGCAACCTGTTGATAAGGCTACGGTAATTGATTCACCCTTTTTAGCAGCGAAAACCAATGCTACTGAAAATGAATTAGCCCAAGCATTTCCGATTAGTCTGGATGATGAAGATGATGACATTAACCCCATTTTAAACAACGATTCCCAACTAGAGGAGGAGAGTGATGATGAATAACGAACACGATAATTTCCAAAAAGAAGTTGAAACTACAACTGAAACAACCTTCAACAGAGAAGAAGGCAAAAGAATGGTACGTCCTTTATTTAAGCGTTCTAAAAAGTACTGCCGTTTTTGTGCCATTGGTCAACTTAGAATTGACTTAATTGATGATTTAGAAGCGTTAAAGCGTTTCTTGAGTCCCTACGCTAAGATTAACCCACGCCGGATTACTGGTAACTGCCAAATGCATCAGCGCCATGTAGCCAAGGCCTTAAAGCGTGCACGTTACTTAGCCTTAGTTCCGTTTGTAAAAGATTAGTTATGAAGGTAATTTTGAAGCAAGATGTTAGCAACCTAGGCAAACGCTTTGATGTGGTTGATGTTAAAGATGGCTATGCAATCCACTTTTTGTTTCCTAAAAAACTGGCTGCACCTTTAACGAAAAAGAGTTTACAAGACCGTGATTTGTTCTTAAAGAAACAACAAGAACACTATGAGATTAACAAGGCGTTAAGCCACAAGTTAAAGGAAGTAATTGAACAAACAGAACTCCACTTTAGCTTAAAGGAGCACAACGGTCGGCCATATGGCTCGATTATTACCAAGCAAATTATTAACCAAGCCCACACTAAAGGGATGGCGCTGCAAAAGTTTATGTTTAAAGACAACGTGCGCTTAGGGTTTGGGGATCACGAGATTACCTTACACATCTTTGAAGATACAACCGCAGTTTTAAAAGTTAAGGTAACCCCGGATAATGGTGTCAAATAAGCTTGAACAGACCTTTAAGTTTGCCAACGACAAGAACATTGAACGTGCTGAGCGTGTTTTAATGCAAGCTTTAGTACAAGATGCCGTTGGTGTGGACTTAATCTTAACCAAGCTTGAACCGAAGGACTTCTTTGGTTTTCCGTTTAACTTTATCTTTCAAACAGCACAGGAAAACTATTCTGAGGGCAATAAGTTGTTTGGTTCGGCGCTTTTAGAGGCCGTTAAGTTTAAGCTGGATACCGATTCCAAAACACAAAGGGAAATTGAAGGTTTATTTGAGGACGTTTTACTCAAAGGTCTTCCCTTTTTGGAAAGGGACATCAAGGTCTTTGTTGATGTTGTCAAAAAAGCTTCGATTTTTCGCCAACTTAAACAGTTCGCCAAAAAGGTTGAAAAGGAAGAGTTTAAGGTCAAAGATGACCGTTTTGAGGGCTATTTACAAGCAATTCAAAACGACTTTACCAAGATTATTCACAGCGCCTTTGTTTCTATTCCCGGTTTGAGTTATGACGAAATTGGTCGTGAAGAGGAAGAGTTAATTCGCAAAGTGTACCGTGGTGAATTAACTGTAAAAGGGCTTAAAAGTGGTTTTTACCCTTTAGATCAATTAACCTCTGGTTGAAAAGAGGGTGAGTTAATTGTGGTAGCAGCGCGTCCAGGTCGCGGGAAAACAGCGCTTCTAATTAACTTTTTGCAGGGCGCGGTTAATGACAGCGAAAAGTTTGATAAAAACAAGGATGTGTTGCTGTTTTTTAGCTTAGAAATGCGTAACCGTGAAATTTATCAGCGCCATTTAATGCTAGAAAGCCAGGTAAATTACACACTCACGAGTCGTCAAAGGCTCGCTAATGTGTATGATGATTTAATTAAAGCATCAGAAGTATTGCGCGATCTTCCTATTAAAATTTTTGATTACAGCACGATTACGTTGGACGAAATTCGTGCTCAAATCACGGAAGTTTCAAAAACCAATAACGTTAAGTTGGTCGTAATTGACTATCTCCAACTAGTTAATGCATTTAAAAACAGCTATAACATTTCGCGTCAACAAGAGGTCACCATGATTTCCAAATCACTTAAAAGCTTTGCCAAGGAGTTTAACGTACCAATTATTGCCGCTGCACAGCTTTCTCGAAGAATAGAAGAACGCAAAGATTCGCGTCCCATTTTGTCGGATTTACGTGAATCGGGTTCGATTGAACAGGATGCTGACATGGTTTTATTCATCCACCGCGTCAAGGATGAGGACAGTGAAAATCAAGAAGCAATTGGTCACAACAATATTTTTCAAGTGGAGTTAATCTTGGAAAAAAACCGTAGTGGTCCGACTGGTAAAGTACAGTTTGACTTTCAAAGTGACGTTTCTACCTTTCGGGTTCGGGAATACAATCCTGATGGTTACAGTTAGTTTATGAAGTACAAAACAGTTAAATCGATCCCGCTTTTTTTGTTAGGCTCGATTGTGTTCACTGCTTGTTCAACACCACAATCAACCTTTCATTTACCTGTGCAAACGACCGTTAGCGCAATCAAAAAAGATATTAGTGGGAAAACAGCTACTGCTGTTAAGGCTGCTAGTAGTTCATCTAGTACTACTACATCTAACGATGACAACAACCAAAAGGGTTACTTCTTAGAAACTAATCGTTCCACCGGTACTTACGACCCTAACAATTCCACCCGGTTAATTAAATTAGGTGAAAGTGGTGATTTTCATGCAGCTGACCAGAATAAACCGGAAGAAGCCTTGTTTGAACGACTTTACGGCGGCATTGCTTCCTTATTAAATTTCCGCATTATTAAACCTGCTTTAACTTATTGAAACACAGTAACACCTTCTCTAAAAGCGATTGGTAAAAGCAGTAATCTAATTACCTTTAGTCAAGATATTGATGAAACGGAGTTACAACGGGCATTGGCTAATAACTTAATTGTCGCTGATGATGGCAACAACAACTTTTGGTTTGGTTTAAAGTCACTTAGCTTTAATGCAGCTAAGTTGACTGACAGCGCCCAAACACAAATGGCGCAAAAAACCACTCAAGCAGTTACCTTGAAAAGTCAAGCGCAAATGTCAAGTACAAATACCAAAAACACCAACAAAAAGATTGATTTGCGCGATAAGATTACTTTATCGAGTACGATGAATACTCAAGGAAGTGGTGATAACAAAAACCCATCTAGTGGTCTTATTCAAAAGTTAGTGAGTGTCGAAAATATTGAAGCTGAATTTAGTTTTGTTAAAACTGGCTTCAACGGTAATGAAATTAAATTTGGCGATTTTGTAACTGAGAATTCACCCACAACCACTCAACTTAAGCAAGTTTGGAAAAAGAAGTGGGGCACGGAATTGAAAAAGACAAACTATAAGCTTCAATTGAACAACTTTAGTCTATTGCTAACTTACACACCAGAAGTTAATAAAGTTGAAAAAGGTAACAATGGTGATTCCAATAAAGGCACGATCGCTACGCCAAACGGTTTTAGCTTCTTGTACCCAGCTAATTTAAATGAGACACCAAGCAGTAGCAGTAGTTATTGAACTAATGTTACTGACTTAACTAAAGCAGCTACTGACACCGAAAATACCAATTTACTCAACGACTTGCAAAAGAGTCAGGAACAAGTCAATCAATTTGTTGCTGCTATTACCCAAAATCACCTCGATGTTTCCGAAGCTGCTTTAACTAAAAAACAGTTTGGTAGCCTTAGCATTAGTGATTTCTTTAAAGCCATCTTTAAGGAAAATGGCAAAGACACTAAAGCTAAAAGTTAAAATTAACCGTTAAATTTTCCTGTAGTTTTAGTTTTCCAGTAGTTTTTAGTATTTGATTTAGTTCTTCTAACTACTTTAATTGAAATTTCCAAAAAAGCGCTGACAAAAATGAGCGTTCCTGACCGGTAGTTTTACCGTTTTACCCACGCTAATTGCATTTTTCTCGATTTTACCGCACAGTTCTGTTTTAGAACCAAGCCACTTAAGGTTCAATTCAACTAGCCAGCGTTACCAATCTGACAACTCCCACCTCTTAAATTTACACGGTGAAGCATTTAGTGATGCTGACTTGAATGCAGTGGCAGAGCATACTGACTGAGACGCTAACTTTGCTTTATTTCAGCAAAAGTTCAAGACAAAGCCAATTACGGTTAACCAGTTAGGTTTAGTCGATGTTTTTAACTTGTTGAGTGGTTGAAAACAGAGTTTGACTGGTGTACTTGAAACAATTCCCCAACTGCAAAAGGAAATAAAAGCTGCTAATGACATTTTCCCTGTCAATGAACAAAGTCAAATTCCCCGTGTAGAAGCACGCTTATTTGACTTTTTGGGCAGTGGCTTTTTCCCGGTATTGAACCCGAAAGGTTTAGGGTTAGCGGACAACGTTGCTAGTTTGTTTGAACAGTTTCAGCTAGCATCAATAAACTTAAGTGGTTTTGAAGTCAAACTGATTAAAACGCATGACATTGTCATTAAGAGTAAGGTACGTTACAGCTTTGAAGTGCAAATGCAGTTCCATGCTGTTTACCAAAATAACGACACTAAAGCTAATTTAGATTTTGCGCTCAATATTAGTACGATTAACTTCGCTACCTTACAAGAATTACAAAACAGTTTTGATCTTCAAGGTAGTGATTTAACGGCTGGGTTGTTTTACAAATACAGCGTTAATAAGTTAACTAGTGGTACTAACGATTTAACCACAATTGCTAAAACCGCTTTAGGTGAAAACATTATCCAAAAGCAAGTGAGCCTAACACAATCAATTATTAAGCCACGCCTAGAAGCTGCAAAAACTCAGTACAAACAGGATATTATTGCACCATTTGCAAAGGAACGTCAAGCTGCTTTGGCACAACATTTAAAAGAAATAGAAGAGGCCAAACAACGTGCCGAGCAATTATTAAAAGAACAGCAAGAAGCGGAAAAACGGCGCCAAGAAGAAGTTAAGAATGTCGCAGAAACACAACAGTTTAATGATAGCTTAACTTCAGCACAAAAATTTAAAGAGTACTGGTTAAAACAAGGTAAGGATGTTACCAAAAAGGTTGAACTCATTCAGGCGCTGAAATCATCTTTCTTCCGCAACCAAAACCGTACCTTTAACTTTTTAATTGCCGGTTTTCGAACTGCCATTGATTGGTACTACAATCAAGAGAAAAATAATACCACTGCTAAAAATAACGCTTTTGGTAAGAATGGCATTCAGTTCCCTGTGGCTGGCTTTCAGGGAATTTACATGTCACAGTGATTGCGTGACGAGCTCTCTGGTAAGACCGACATTAAGCTCAACTTAAAAAGTTTAAGTGTTCAGAATGAAAATAAAAATTCTTCCATTAATTGGAACAAGCAAAAAAGAATTGAGATTAAACAAGTAAAACCATTTAACTATTCTTTTGAAATTAACTTGAAGTACACTGGTAGCTATAATGTCAGTCTTTGATATCTAATTGGGGCTGCAATTGGTGGGATTCCAACATCTTGAAGTGGAACTATGGATATGAAGTTTATCGTTGATGGTGATTTGGATAGCGGTATTGTTACCAAGCAAGATTATCCCGGTTCTAAGTTTGAATTTACCGAAGACAAACTGTGGTTTACCCTGCACGTTAAACAACAAATTAAAGTTAAAGAGCAGGGCTTTATGAACTTGCTTAAGGGTCAAAGTTTGGACAACTTGGATCTACGCACTGGTACAACTAAACCGCCAGTAGTTGATTTAGCGAGCTACCTCCACTTTGTTATTTTGACAGCTAAGTAGTTCATTGAGCCGCAAAGGCAGCAAATGGAACAACTTTTAGACCAGTTTTTAAGTGGTGTTTTAAGCGAATGGAAGGCTTTTATTTTGCAAGAAGCCAAACAGACCTATTTTCAAGAATTGTTAGCTAAACTAAAAAATGTCGAGCAAGAGCTAACTCCCAAAGCCTCTCAAGTCTTTCGTCCCTTTAGCTTTTTTGCACCAAATAACACTAAACTAATTATTTACGGTCAGGATCCATATCCTAATCCACAACACGCTTGTGGTCTTTCGTTTGCCTCTAACGCCCCTAAACTACCACAAAGCTTAAAGCGGATGATTCTGCGCTTGCAGCAAGAGTATCCGGAACTAGCAGGTCAAAATCACTGAACGAAGCAACTGTTAGAGGGTTGAGCACAACAAGGCATCTTGTTGTTAAATGGCGTCTTTACCACCAATGCCTTTCAAACCAATGCGCACCGCAATTGGGGGTGAGAGCAGTTTAACTGTCATTTACTCGATTTTCTCTTGTCACAAAAACTCTATGTACTACTCGTTTTTTTGGGCAAACAAACCGAGAATTTTGTTCTTAAAAAGATTGGTGGAGCAAGCCAATTTGCTAGTTTATCCTACCCCCATCCCTCACCTTTAACCGGGCGGAAGTTTTTCGATCATCCCGATGCTTTGTTTAAGCAAATTAACCAGTGATTAAAGCACCATAACCATACTCCAATTGATTGGACTAACGGCCAGGTACAGTACCAGTTCTAACTGTTTTGGTAAAGTTTTGTCTTTAATTCAACAAACAAAGCTTTAGCATTTTTCATTATTTTTCTTATGATTTTTATTCCGCTTGTACTCAAACAGAAAAGCACAGCGAGCAGTCTTTTCTTTACAATGTAAAGAACTGCAAAGCTGCTTAAACCGTCTTTGCAAGCTTGTTTTAGAGTTGCAAAGCAAAGTCCAAAATGGATAAATTAATAAATAAACCTCAACCATTAACTTCTGATATTTCCACTTCTGGATTTATTTACTTTGCGGTTGTTTTTGTAGCCATTATGGGCTACCTTTTATTCAAAAATCTACTGTTTTTATTTTTCTTTAAACGCTATCCAAAAAACACTACCAAAATTGGGGTAGGTAACATTACCACGATTGCCATGATCATTGCTGTGGCGGTGTCGATTGTTTTAGTGTTAATGGCACTAGCAGGCGGTTTAGCCGCTGCTTTGTTCCGTGGTTATCCTGGTTTTCGGGTCACCTTGGAGTTAATCCTTGTAAAGATTTCGGGACTGTTGTTTGGTCCAATTGTCGGCATTTTTTCCGCGGCCACAATTGACTTTTTAACGGTCATCTTTTCGGGTGGGGTTTTTAACGTTGGTTATGTTTTAGGTGCCATCTTAACCGGGATGATTGCTGGCATACTGCGTGAAGTTTTAATTTCAACAGCACTTTTACACAACCGTAATTTATCGGATTTTGCCTACCTAGTGCTCTCGATTGGCATGGTCATTGCCGCCTTTTTAATTACCCAGTTCTTTGTGCTTGGGATAAGTAATAACCTTAAGGAGATTAAGGGTGATGAAGAGTTTCGCTTGAAATTTAACGCACCACCAATTGTCTTTGAGCTTTCGTTAACCCAGTACGCCAACATTCTGCTCTACTTTACCATTGCCATAGTTATTGCTATGCTGGTGTTGTACATTGTTTGGTTAGTCAAACAGCGTCACTTAAGTTTTGAGCACTCTCGTTTCTTTTACCGCAGTTACAAACATGCTAATCACCAGTTTACCCTGTTTGTACTAACTAAGGAAAACTGGTTCTATTTAATCTTGAATGTAATCACCTTAGCTTCCACTAGTTTGTTGATGATCAACATTGCCTTCATTCCGATCTTTGACACGCAAACAACTGGTCAAACATACGAGTTTTGACTATTAGCACGCTTACTGTTCGCTCCCGTAATCTTCTTGTTGGACATTATTGTGATCTATCCAATTCTGCTGTTATTAACCCCGCTAATGCTCAAAGGTTTTAAAACAGCAGTTTCTAAAAACCAACGCAAAACACTAAAACAAAGCTTTACTGATTTGCAAAGTGTAGTGTTGCCCATTATTAACAAACGTAAACACCAGCAGTTGCGCCAAGAAGAGTTAAAACGCTTAGCACGAGCCACTCACTTTGATTTGACAGAAGGCGAAATGGAAAAGCTCTTGGTCGAATTTAAAACTATTACCCAGAGCTTTGACCGGGTGATGAACATTGATACCACCAGTGTGGAACCAATGTACGCACCTTTCAATACATCACCAACGCCACTGCGTAAAGATAAGGTAATAGTGGAAAAGCATCCCGAAAAATTATTAGCCAACTGTAAGGAAATGAGTGTTGGCTTTGTCAAAGTATAGTTGTGAAATCGCAAATCTTAAAGCTCCAGCAAACACTAACTAAAAAACCAGCGTCAATCAATCCGTTATTACAACAAATTGATGGAGCAATTAACGAACACTGATCGAGTAACTTTTTGTTGAAAAATACGGTTGAGTGAGCACAAGCCCAAGCACCCAAAAACCGCAGTAAATCACCACTCAACAACATTCCCTTTGTTTTAAAGGACAACATTGCCACGAAGGGAATTGTTACTACTGGTGGTTCGAGGTTTTTGGAAGACTATATCCCGCCGTTTTCAGCAACTGTGTTTGAATTGCTAAACAACAGTGGCGCTTTATTAGTTGGTAAAGCGAACCTCGACGAGTTTGGTTTAGGCGGTACGGGGCTCCATTCGGGTTTTGGTTTTGTCCACCACCCTTGAAACGAAACCCTAATTCCTGGTGGTTCTTCATCCGGTTCCGCTTATGCAGTGGCGCGCGGCATTGTTCCCTTTTCAATTGGTACGGACACGGGAGATTCGGTACGCCGTCCAGCTAGCATTTGTAACATTGTTGGTTTTAAGCCCACCTATGGTTTGATATCGCGGAACGGAGTGTATCCTTACGCGCCTAGTTTAGACCATGTCGGGATCTTTGCGCGGTATGTGTATGATGTAGCACTTGTTAGTGATGAAATCATTAAACATGACAAAGCTGATTTTTCCGCTCAAAAATCTCCTGATGCTGGCAAGTTTACCAGGTCCTTAAAAGAGTCCTTCAACAAGCAAATCAAGATAGGTTATCTAAAACCGCTAGAGGAATGGTTTGACATTGAACTGAGCAAAAAGTGAAACAGCTTAAAGGAAAGAATTACCTTGGAGGGTTGTGAGTTAATTCCGTTTCACTTTCCGCTTGAACTGCTAGAGGTAATTGATCCAGTTTACAAGCTGATTAGTTACAGTGAAGCGGTGAGCTGTTACAGTAATCTCACTGGGATTGTCTTTGGTCAAAAATTGTTTGAACCCAACCAAGCGAGTGACTTTTCCAAAACAATTACTGCCAACCGCGACCGTTTCTTTGGTGAGCAGTTAAAACGCCGCTTTATTATTGGTGCTTTTGGTACTGACAAGAATAACTTTACCAAGTACTTTGAAAAGGCTCAAAAGATCCGCCGGGTAATGGTTGATGCTTACTTAAACCTGTTTAAAGAAGCGGATTTCATTGTGTCACCTAGTGCATCCGGCTTTACTAAAACAATAGCTGCGGTGCAAAAGGGTGAGAGCTTTACTAACCTCGTGGATGACTTTTTACAACTAGCTAACTTTGCTGGTAATCCTTCAATTACTATTCCCTGATTGGTCAAACAAAAGGACCAAACGATTGGTTTGAGTGTTAATGCTAACTGCTTTCACGACAAGCAATTATTACAAGTAGCTGCTTGATTGGAGGAATTATTCCAAATTGAACATGATGATTAACTTTGAAGCAATTATTGGGATTGAGGTACACGTGGTCTTAAACACTGCTACCAAAATGTTTTCCCCAGCGCCCAACCAAGCGCAAAACGCCACACCAAACCAGTTCATAAACGCGATTGATTTGGGCTTGCCTGGTACCATGCCCCAGGTCAATGAGCAGGCGGTACAAAAGGCGTTAATCCTTGCTGACGCGCTCAACATGCAACGGGTACAGCCAGTCTTAGTGTTTGACCGTAAGCACTACTTTTACCAAGATCTACCTAAGGGTTTTCAGATTACCCAGCAAAACTTTCCGATTGCGCAAAACGGTTACGTTGAAATTGTGGAAAACAACAAAGCGCAGCGGATTATCATTGAACGGTTTCACCTAGAGGAAGATACTGCCAAGCAACATTTTGTCGATGGGCAAATTCTGCTCGACTTTAACCGTTGTGGTGCACCGCTAATTGAAGTAGTTACCGCGCCCGTGATTAAGAGTGCTAAGCAGTCTAAGGCATACTTGCAAAAGCTACGTCAAATTTTGATTGTTAACAACATTTCCAACGCTAAGCTCGAAGATGGCTCGATGCGCAGTGACTGTAATGTATCAGTGCGCTTAAAGGGACAAACTGCTTTTGGCACCAAGGTCGAAATTAAGAACATTAACTCACTCAATAATGTGGAAAAGGCAATTGATTTGGAAATAGCACGTCAAGTCAAAGCACTAATTAAAGGCGAACCGGTTCAACAAGTTACCCTAACTTATGACGACAAAACCAATACCAATGTCTTCATGCGTAAAAAGGACAATTCAGTCGATTACCGTTATTTCATTGAACCCAACATTATGAGCAGTAACATTGATGAACTGCTACAAAAACCCAACAAGGCCTTTAACTTAACTGAGTTCTTTACCAAGCTCAAGGAAGCTGGGGTTAATGAGCAGCTAAATCAATTAGTAGTCGATGATCTGACTCTTTTTAATGCTTACACCAAGATTAACAGTGTCATTAACAGTCCCCAAGATACCATTCGCTGGTTGTGCATTGAGTTAATGGGACAACTCAACAAGCTACAAAAACCGCTCAAGGACAAAACGATTGATCACTTAATTATCCTAATCCAAATGGTACAGAAGGGCACCGTGAACCAAAAACAAGCAAAGCAGTTAATTGAGTTAATGTTAGACAATGGGCAAGACCCCCAATCGCTGGCTAAACTACACAACTTAGAACAGATAACTGATGAAAAACAACTAACGCAAATCATCCAACAAATCTTTAAGGAAAACGAGGGTGAAATCCTTAAGAACTTAGATCGAGTTGAACGAATTCAAAAGCTAATTATAGGTCAAGTTATGCAAAGAACCCACAATCGCGCCAATCCCCAGCAAGTTTTTATAATTGTTGAAAAGTTGCTTCATGATTTCAGCGAAAGAGCAAGCTAAAAAAGGTCAAATAATTTACCGTTACATTCTCCTTAAAATTCAAAGCTTTAAATGGCCTGCTGGCACCCGCATCTTCTCTGAACGCCAGCTCGAAATTCGTTTCGCTTCATCTAGAAGTCTCATTCGTACTATCCTCAATACCCTCTTAGGTAAAGATCTAATTCGCCACACTAAAGGTAATGCTGGTTACTTTGTCGCTAAGAATGCTGGTTTTAGTTTCTTTCATAAAACCCAGGACAACAAATTGCCCAAGTGAGCTAAGCTCTCAACCTTAATGAAGAACCACCTACGCGAAGTTGACCGTGATGTTTTCAGCTCAATTGACAGTGGCGTTGACTTTGGTAACTTTAAGGGTTTGGAAGCCAAGTTATTTGATGAAAACAAGAAGAATTTTTTAAACCTTAGCTTCTTTGGCAAGGATGATGTGCTCCAAATTTTTAGCGAGCAAAACCTCCAGGAACAATTCTTTAAGGACTTTGCTTACAACGGCATTGTGGTAGAGCGCAAAAGCTCCTTAATCTGTGTGGATGATGAGACTAAGAACTTACTGATCTACGACCTCTTTTATGATGACAACAATAAGTTCATAGTAGCAATGCGCTCTGAATATTTAAACCCCCGCATCAAAATTATTAATGCTTAAAGTAAAGTCAGATTTTTTAACTAAAGATCAGGTTATTTACGATGTAGCGATAGTGGGTGCTGGGCCAGCTGGCATAGCAGCGGGTATTTATGGCAAACGCGCTAACCTCAATCTAGCCATTATTGAAGGTAGTACTCCCGGCGGTAAGGTTGTCAAAACCAACATTGTCGAGAACTATCCGGGTTACAAGTCAATTACCGGACCTGACTTGGGGTTGGAAATGTACAACCACCTAATTGATTTAGAGCCCACCTTTTTCTATGCTAACCTAATTAAGTTAGATAAGGCAGCAGATACCTTTATCTTGTACCTCGATGACAAAACTGTGGTGTTTGCCAAAACCGTTATTTACGCTACGGGAATGTTGGAGCGTAAACTGGGCGTAGCCAAAGAAGACCACTTTTACGGTAAGGGAATTAGTTACTGTGCCATTTGTGATGGGTCCTTGTACAAAGACCAGGTGGTGGGTGTTGTTGGTGGGGGGAACTCTGCCATTCAGGAAGCGTTGTATTTAGCTGGCATGGCTAAAACGGTGCACTTAATCCACCGCCGTGAGGGCTTTCGTGCTGATGAAACGGCGTTAAATAAACTGAGAAACTTACCGAATGTTGTTTTTCACTTGAACTATACCGTAAAAGAATTGTTGGGTAACAATACCCTAAACGGCATAGTGCTTCAAAACACGCTCGATCATTCAACAAAACAGATAGATCTCAATTGTGTCTTTCCCTATATTGGTTTTGAAAGTATTACCAAACCGGTGGAACACCTCAACTTAAAGTTAGATCCCCAGGGTTTTTTAATTACCAATGAACAGATGGAAACAAGTTTAAAGGGTCTGTTTGCAGCAGGTGATTGTCGTTCTAAGCACTTCCGTCAAATTGGTACTGCCATTAATGACGGCATTATCGCGGTGTTAACGATCCGTGATGTCCTTTAGTGTTCAGATTAAGCACGAGTTAGCCACCAACCCTTTAATTAAAACAGAGTGATCCAGCTTTTTAGCTGGTTATTTTCAAAACGGGTTAAAACTACTCGCTACTGGTCAATGATCCTTTAAAACTAGCACTGCTACTTTAAAGGCGTTGTTTAGCAATGCACTCCAGTTTAGTTTTCAAATCCACGAAACGAGCACTCACTGTGAGTTTAGTTTTAGGGCTTCGCAAACAGAGGTGGATCAGTTGTTAGCCTTTGATGCAACTCAGAGCGATTTACCGCTACAGAAGGCCTATGTCATTGGTGCCTTTTTGAGTGGTGGTAGTGTGAGCGATTTACTCCATTCCAGTAACTTTCACCTCCAAATTAGCACTAACAACGAACTGCAAATAAAACAGTTAATGAAGCTGTTTCATCCTTTTAAACAAACCACTAAGCGCCACCAGCTTTTGGTCTATGTTAAAAGCTATCAGGCCATTTGTGACTTCTTTAAACTAGTCGAAGCTTTTGATGGTTATCTAGCCTTTGAAGAAAACCAATTGCAAAAAAATTTTGCCTTAGAACAAGTGCGTAAAAGTAACTTAGAAATTGCTAACCTAATGCGCACTTTAAAGACCAGCACAAGCACAGCGGAACAGCTAAAAATCCTCATAAACAGTGCTGACTTTAAAAAGCAATCGCTCAACTTTCAGCGCTTTTGTTTAGTCAAACTAGACCATCCTGACTGGTCATTAGAACAAATTGCCCAGTTTTTTGAAAGGAAATACAAAGTGCAAATTACCCGTTCGGGAATTCAACACCTCAACGCTAAGTTAAAAAAACTAAACCAAAATTAAAGCATTAGTTTAAAATTAACACACATTAAAGCATGGACGCAATTCAAATAGTAATGTTTGTAATGGCAATCCTTTGTTTAATCATCGGTTTATTGCTATCCAACCATGGTTCTACTGGCGGACTTGCTTCGCTTTCAGGTCAAGATTTGGAAATTTTTCGCAAAACCAAAGACCGCGGAATTGTCAAAATTCTCCAAATTACGATGTTTATTTTAGTAGTGCTTTTTCTTATTTTAGGGTTAGTTTTTCATTTTGCGCTTTAAAATCTAACAGATGAAAGTATTAACTGATTTACAAAAGCGAATCTTCGCGATTGTGAAAAAAGAGAACGGCAAACCTATTCCTCCGGGAATAGTTGTCAGGATGATGGAGAATCAAGCTGGTTTTCCGGGAAAGCAACAGGTATACAGAGCAATTGATGACTTGTTAGAATGACACATTTTTCGCAAGTCGGGTGGTGCTACAAACCAGCTGTTAATTAACTATGAACTAGCTGATCCGGTATTGGATCAAAAATTTCAGGGCATTCTCAATTTAGGTAACAAGAACACTGGTTTTGTCCGTCCCTTAGATGATGACAAAACGGTGTACTATATCCATTTTTCCAACCTAGCGGGAGCACTGGATGGTGATCTAGTTGAATTCTGTCCGCTCGACAAACCACAGGTAGGTGATAAGTTTGATGCTGCAGTGCTCAAAATTGTGAAGCGCTCCCGGGTGCTGTACGCTGGTAACTTTTTGATTGAGTACTCCGATTTTGGTCAGGAGTTTCGCATTGTTGCTGACAATCCTCGTTTTTACCTCACCCCGATTGTCAATAAGGCCTCAGTTCCAGCTGAACTGGAGAGCAATACTAAGGTCGCTTTTCAAATTGATGAGTATGATCCTGCAAATAACCTGTGTAAAGTCTCCATTCAGCAAATCTTGGGGAATAACGATGAACCGTTAATTAACCTCAAGGCGATTATGTTAGACCACTCGATTGTCTTTGAAAAGAATGATGTGGTCGAACAACAAGCAGCGAAGTTACAGTTTGATGAAAAAGAGCAGAGTAAGCCCTACCGCAAGGACTTAACCGAACTTGCCTTTGTCACGATTGACCCCGCCACTTCCAAGGACTTGGATGATGCGATTTACGTCAAGCGCACCGACAAAGGTTTTGTTTTGTATGTTGCCATTGCTGATGTGGCTTACTATGTGCAGCGCAACAGTGAACTTGACATTGAAGCCCGTCATAAAACAAGCTCAATTTACCTCCCGGGATACTATGTGGTACCGATGTTACCAGAACGTTTATCAAACGAGCTGTGTTCTTTAAACCCAAATGAAAAGCGGTATGTGGTGGTGTGTGAGTTGAACTTTGATCATGAAGCACGCTTAAACTTTAGTGAAGTGTATCCCGCTACCATTGTCTCCCAACGTCGCTTTGCTTACAGTGAAGTTAATGATTGACTGGAAGACAGTGATGCTCTAAAGGATGAAAGTGCAACAGTACTAGAGAGCTTAAAGGCTGGTTTTACCTTGAGCGAATTGATTGCTGAACAGCGCAAAAAGAAGGGGACTATTGACCTATCTCACAGTGAAACAGAAGTAGTCGTTGATCAAAACTACTACCCGATTGAGATCCGTTTTCTCACCCACGGCAAGGCGGAAACAATGATCGAAAACCTAATGGTGGTTGCCAATGAAGCGGTGGCTTGAACACTTACAAACCACAAAGTTCACTTACCTTACCGGGTGCATCCCCGACCAAGCAAAAAGAAGTTACAAATGTTGTTGGAAAACATTGTCGAGCTCAAAATTACCCAACCAAACTTTGTACTCGATACGGTCACTTCCACCCAAATTGCGGCTTGGTTAAAGGAAAATAAGGACAACCCGAGCTATGACATCTTTGTCATCTTGTTATTAAGAACGCTAGGTAAAGCCTTTTACATTGTTAATCCACTGATACACTTTAGCATTGGTTCACACCACTATACCCATTTCACTTCGCCCATCCGTCGCTATGCTGATTTAACGGTACACCGTTTGTTGTGGATGAATCTGTTTACTCCAGAGCGTTTTACTGACACTGAGCGTGAACAGCTCAACGCCGAGCTAGAACAAATTTGTGAAACCATTAACGATACTGAGATTAAGATTAATGGTTGTGAACGGACTGCCAACGATTACTTAACTACGCTGTATTTGTCTAAACAAGTGGGACAAACTTTCCACGGTTTTATTTCCGCTATTACTAGTTTTGGCATCTTTATGCGAATGGATGAAAACAACTTTGATGGACTTATTAAGATTACCTCCATTCCCGAAGACTTCTTTGTCTTTGAAAAGGATCGGATGGTGTTGCGCGGCAAACGCACCAACAAGGTTTTTAGAATTGGTGATAGGTTAACCGCGAAACTGACTGAAATTGATACAGTGCAGAAACGCGCCATTCTAACCCTGGTTTAATGATGACAGTGGAAGTCTTTTCGGTTATTATCCTTGTCCTTTTATTCTTAATCTTAGCGCTTACCTTGTTGTTTGTTTTACTCAACAAGCGTACTAGAAGCTTTGTTATTCGTACCTTTACTGGATTATTTAGATCAAAGCATACTACCAGTCAAAAGAACTTTTATGACAACTTGACCTCAACCTTACTGAGGTTATCAACTGATAAGATTGGTGCCATTATTGCCATTGAAAACCAGGACTCACTCGAGTCTTACGTCAATATTGGCTACCGCGTGACTTCGGACTTTTCCCCCGAGTTGTTAGTAACGATCTTTTACAACAAGCAGTCGCCACTTCACGATGGAGCGGTAATTGTGCGGGACTACCAGATTGTGAGTGTGTCCTCTTACTTCCCAATGACACGGCAACTAATTGATGTGTCATACGGTTCCAGACACCGCAGCGCGTTAGGTCTAACCGAAAAGTGTGATGCCATAGTCTTTATTGTTAGTGAAACAACAGGCAAAATTTCGGTGGCTGTGCGCGGCGTGATTAAAACACTGTCGAGTAACTCCGACCGTTTACAAGACCAGATTATTCACTATCTGACGGTAAAGCCGGGTTAATTACCTTCCACTCTTTATTTGTAAACAAAGGGTTTAGCGGATTAATGCGGTGGTAGTAAAACCGCCCTTGCAAATGATCGAATTCGTGTTGCAAGCACATCCCAAATAAACCAGTAGCAGTAATGGTGATCTCTTTTTGTTGGAGTCAATCAAAACCAGTGATCGTAATCCATTCGTGGCGAATAACATAACCCTGGTGCATTTTGGGAACACTCAAACAACCCTCACCGCTCTTTAAAAAGGATTTGTTGGCACTTAAGTTGATAATCTTGGGATTAATTAACAGACACTTGTGTTCCACCCCACCATCCATTAGGTGGATGTAGAACATTTGCTTTCAATAACCAATTTGGTTAGCGGCAATCCCAATGCCGGGAATAATGCCATACTTCTCAGCATCACCGTTGTAAGATGCATCAACGTAAGCCATCATTTTGGCAATGCAATCGAGACTCGCTTGGTCTAAGGGAAACTGAACTGGTTTAGTCGGTTCGTTAATTTCCTTAACATCGTCCAGCACTAGTCATGCTTTAGTTGGCAATAATTCCATATTAATTTGCGGTAAAATTAAAATTATTCTAAATATTGAAATGGTCGATACAGGGAGAATTTTTGTCATTACCGGACCGAGCGGTGTTGGCAAAAGTAGCCTTGTTAGATGTTTAATTGACCATTTCAAAGATAAACTGCGCTACAGCATTTCCGCTACCACCCGTAAAATGCGTAACAGCGAAACTGAGGGTGTGGATTACTTCTTTAAAGATAAAGCGGAGTTTGAAAAACTGATTGCTGCTGATGCCTTTGTGGAGTGAGCGATGTATAACGATAACTACTATGGTACCCTTAAATCCCAAGCTGAACAAATTATTCACAACGGTGGCAACTTGGTGTTAGAAATTGAATATCAAGGTGCTTTACAAGTTAAGCAAAAGTATCCCAATGATGTGGTGCTAATTTTTATTAAACCACCTTCAATGGAAGAGTTGTTAGTGCGCTTAAAGAAGCGTAATGACGAGGATGCAATAACAATTCAAAACCGATTGAAACAAGCTGAGAAGGAATGTCAACAAATTGGTCACTTTAAGTATGTGGTTACCAACAACGAGTTTGACAAGACCTTAGCGGAGTTACAAGCAATTTTACTAGCTGAATTTAATTAATGGACAGCACCAACCAAAACCTGTTCGCGTCATTGTCCAAAAAAGGACTCGTTCGCAAAGAAAATCAGGACTTTAGTGTTGTTACTTTCAACCGCTTTGGTCAGTTAATGAGCCTTGTTTGTGATGGTTTAGGCGGTTATAAGGGCGGTAAGATGGCTAGTGCCTTGGTGAGTGAAGTTTTTACCAAGTCCTTTACAGTCTTTGATTTTCATAGTCAAACTGAAAGGGCAGTGAAACAGTGGTTTGAAATTACTTTAATTGAAGCGCGGCGCACCTTAGAACAGTACTTTCAAACAATTAAACGTAACCAAGTCCAGTTTGCGCGAATGGCGACTACCTTAGTGTTGAGTATTATAAGTAAGCAAAACATTTGAACCTTTTGGGTGGGTGATTCACGTGCTTATTTAATTAACAGTTACCAAAGCTTGCAAATTACCGAAGACCATAACCTGTACAACCAACTGTTACAGATGCACGCTACCCCTGATGTGATTGCCTCTTATAAAGACAAGCTGTTAGCCCTCACTGCTACCGTTTCCAAAGATCAGGAACGCCAGTTGAAATACAGCTTTAGGTGTGATGTAGTAAACGCTTGGGACTTTTTACTGTTGTGCTCTGATGGTCTGTACAACTTCTTGGATCCCAACTGCTTTTATGAGGTCATTACTAGTGCACCTAACCTCAAAAAGGCCGTTACACAACTAGCAAAGTTGAGCTTGGATAACGCTAGTAATGACAACATTACGCTCAACTTGATTAATTTAAAACAATGGCACTAAATTTAAAGATTGGTGACATTGTCCAAAACAAGTACCGGATTGAAAAGCTAATCAACCGTGGCGGGATGAACTCTTACCTGTTTTTAGCGAGCAACCTCCACGTCCAGGAATTTGGTCCACTGCAAAAGCGCCAGTTTACCCGGTTGGTGTTAAAGGTAGTACAACGCACCGACAAGATTAACGACAACAACTGGAAGAAGTTTTTGGATGAAACCATTACCACGACACGGGTGTCACACAAAAATCTGGTGCAAACCTTTGATGTAGTTAGCCCCCGTTTAAGCGTTTTGAGTGAAAACCAGGTGATCGTTTTAGAGGACACGGTGATGATTGTAATGGAGTATGTTGATGGTCCTTCTTTGCGTGAAATGCTCAACCAGAAGGGTTACTTTAGTGTGCAGGAGGTGGTGTACTACTTTACAAAGCTAGTCAAAGTAATTAACTACCTCCACAGTTTTGAACACCAAATTATTCACCGTGATCTAAAGCCAGAAAACATCCTGTTTACGAGTAACTTGACTGACATTAAACTGCTTGACTTTGGCATTGCTTCCGCTGTTATTCGTAACGCTGAGAAAACTGAAGTGTTAACGGACGAAAACTCGTTGTTTGGTACAGTTAGTTATATGACACCCGAAGTGTTGGAAAGTACTGTCAACAAAGAAGGCAAACGGATTCGTAAACCCCCTACAGTGCAGTATGACATCTATTCATTAGGAGTTATTTTGTTTGAAATGTTAGTCGGACGCGTACCGTTCAATAAATCGATTGATCCTAAAAAAGAACGGGAAACCATCCAAAAGGCGCGTAACTTTGATGTACCTTTAATGGGAAACTTGCGCAGTGATGTTCCGGTTAGTTTGGAAAACATTGTCTTTAAATGTACGGCTGTTAAACGTGAAAACAGTAAGTGGATGTATAGTGACACGAAGCAACTCTTGGCTGATTTGGCGCAATGACAAACCGAGCAAACCTTAATTAAACCAGTGCACGAACGTATTCTAGAAGGACAAAACGAAATGCGTGAACTAATGGTAAGTAATTACCTACCGTGGTATTTAAGGAAGGGTGTTTTGATCTTTTTTTCTGTTGTTTTATTAGCACTCTTAATTGCTGTAGTTAGCTTTTTTATTATTACTGGGGTAGTTGTCCATTCGTAAAACTGGCATAGTATTTCAACGCTTTGGAAAGCAGTTTAAGGTGTTTGTAGAAAACCAAACACTAGTTGCTTTTGCACAGAAAAAACTACAGTGAAAGCGCGATTTTAAACTGTTAGTTGGGGACAAAGTTACCCTTGAGAATGGTGTCATTGTCGCAGTTGAAGAGCGTAAAAACACACTGGTGCGCCCAAAGGTAGTTAATGTAGATCAGGTAGTAATAGTCCAATCACTAATTGAACCCAAGATTAACTGACAACAGTTGTTTAAATTGTTAATCCATTTTCACGCTCAAAACGTGGCACGGTTAGTGTTAGTCATTACTAAAAACGACCTTGAGTTTGAACCTGCTGAAAAAGCCCGTTTAAGTGAACTAACCAGCTTTGGTTACCAACTCTTTTTTACAGCCCCTAATGCTGATTTACCTCTCACTTTATTTACAGAATTGCAAAACCGTTTTAGTGTATTCATGGGACAATCAGGGGTCGGTAAGTCTAGCCTTATTAACCGTTTGGACAGTCAAATTCACCAAGCGATCCAAGCGCTTTCAGCACACCAGTTCGGTAAAAACACCACCACTTCCACGGTGATGTTTCCCTTCCAAAACGGCTTTATTTGTGACACCCCTGGCTTTAATGTAATTGACTTTCCCAACCTCAAACAATTAGCAGCCCAACACTTTGTCGGTTTTGCTAGCTTAATTGGTCAATGCCATTTTTCCAACTGCACCCATCAAAGCGAAAAAGGTTGTTTTATTGTAAGTGCTGTAACCCAAAAATCGTATTCCTTGTGACTTTATGAGAGCTATCTAAAGTTGATTAATTAAAATCAACTAAAAAAAGGATTTATGGAAAGTAAATGGTTAACAGTTGACACTAAGCACCTCTATGGTTTTGATGAAGCGATTTTTATTCAGAAATACCAAAAAAAAGTTAACCAAATTCACCAACAGTTTTTAAATCAACAACTACCCGATGGTCACATGAATGGCTGGTATAGTCAACCCGATCAAGACCACAAGGGTTTGCTAAAACAAATTAACACAATTGCCAAACAGTTTAATGCTCTAAAAGTAACTGACATAGTGTATTTAGGTATTGGTGGTTCTTATACTGGCATTCGTGCCATCTTAGACTTTTTAAAACCAGAACAGAAGGCGAATATCAAAGTGCACTTTGTTCCTGACATATCGGCCTTTAACATTGCTGCAGTTGCTAAAGCAATTAAGGGTAAATCATGAGCTTTGGTAGTAACTTCTAAATCTGGTCGCACACTCGAGCCGGCTGTTACCTTTCGGTACTTCCGTAATTTATTGCACAAACAATACAAGCAAAAACATGCTTTACGCACAGTTGTGATTACCGATGCCGTTAAGGGTTTACTAGTTGGCATGAGTAACCAGTACGGTTATGCACACTTAACTATTCCAAGCAATATTGGTGGGCGTTTTTCAACTCTTTCACCCGCCGGTTTACTGTTAGCTAAACTTTGTGGTCATGATCCGAAACAGCTCTTATTGGGTACTTTGACAGCCAAACAAGAGTTAGCAAACAGTGATTTAAACACTAATTCGGCTTATTATTATGCTGCTTTGCGTCACTGACTTTACACGACAAAGAAGCTCAAAATAGAAGTGACGGTAGCATACCACAGTGCTTATGAGTACCTCTTGTTGCAACACCGCCAACTGTTCGGTGAATCAGAGGGTAAAGGTGGTAAGTCCCTGTTCCCTACCTTTTCGCTATTTACCACTGACTTACACTCAATGGGGCAGTTGTACCAAGAAGGGGAAAAGAACTTCTTTGAAACAGTGATACAAGTGCAAACACAATTTCACGACTTGGAACTACCACCATCAGACTTTAACAATGATGATCAGCTAGATTACTTATTGGCTAAAAGTATGAATGAAATTTCAAACACTGCCCTAGAAGCAGTTGTGGAAGCTCATTTTCAATCAAACGTTAACATCATTAAGTTAACGCTCAAAGAAAGAACAACCTTTATGTTTGGTTACTTTTACTTCTGGTTATCAATGGCCACAATGATGAGTGGTTCACTGCTGGGACACAACGTCTTTGATCAACCAGGGGTGGAAGTGTACAAGCAGTTAATGTTTGCTAAACTTGGTCGTGAATAGGGAAATTGCCTTTTCGCTTTTACCACTGTTACACCAGTTTGACCGCAAGTTGTTAGAACAATTCTTTGCGGACGGCTTGCGCTTAATCCACTATGACGTGATGGACCATTTTGTGGACAACACTGTCTTTCAAGGGGAACATTTAGATGAATTACAACAAATTGGTTTTCAGGTCAATGTACATTTGATGGTACAAGCATTAGAACAAATCTTGCCAGTATACCTCCACCATCAAGCCGTTAAGCGTATTTCCTTTCACGTTGAACCGTTTGATATCCCTACCATCAAACACTTTATTGCGCAAATTAAACAAGCGGGAAAACAAGTCGGTTTAGCGTTTAAATTCACCACTCCGCTTGTAAATTACGAGCGCTTGGTGCAACAACTGGACTTTGTTACCTTAATGAGTGTCCCCCCAGGAAAGGGCGGGCAAGCCTTTAACAGTGCTGTTTTTAATAACTTAAAACAAGCCCACAAATACCACTGCTCAATTGAAATCGATGGTGGGATCAAATTAGATAACATTCACCAAATCCAGGATGATGTCAATTTTATTGTCATGGGAAGTGGCTTTATAAAATTAGAACGATGGCAGCGCCAACAGCTGCTCAAAACCAATCAATAACTTTATATGTCTGCAACTGCTATTAGCGATCTCTTTGAAAAACCAGCCCAATTTAAAAACAAGAAAATTAAGCTAACGGGTTGATTGAAAAACAAACGTACTAGCGCCAACATTATCTTTTTGGAAGTTAATGATGGATCAACGCTGTTAAACCTACAAGCGGTGGTTAAACAAGACCAACCGGAGTTGTTTGCATTAGCTGAATCAATTAGTTTAGCGAGTGCTGTAAGTGTTTCTGGTACTGTAGCGTTAACACCCAAATCCAAACAACCCTTGGAATTAGTAGTCAAACAAATTAATGTATTGAGTACTGCGCGAGCAGACTATCCACTCCAAAAAAAGGAACACAGCTTGGAGTTTTTCCGCAACAACGCCTATTTGCGCGTACGAGCCCGTACCTACTTTGCCATCATGAAAGTGCGCAGTCTGTTGTCACAAGCCATTTTTGACTACTTCTTTAAGAATGACTTTGTTTTAGTGCACAGTCCAATTCTTACTAGCAACGACTGTGAGGGGGCAGGTGAGACCTTTGAATTAAAGCAAGGTAAGGAATTCTTTAATAAGACTACTTACCTTACTGTCAGTGGTCAGTTTGGTGCGGAGTGTTACGCCCAAGCCTTTAAAAAAGTGTTTACCTTCGGGCCGACTTTTCGCGCTGAAAAATCACACACTTCACGGCACCTTAGTGAGTTTTGGATGATTGAACCAGAAGTTGCTTTTGCCAACCTCAAGGACTTAATTAAGCTAATTGAAAGCACTGTCAAAACCGTGATTAAACAGGTTATGCAAAAAGCCAAGCAGGAACTCGACTTTTTAGAAAAGCAGTTTGATGTCAAATTAATGGAACGCTTAAAGCAAATTACAAGTACCAAAAACTTCCATGTTTTGGAATACATCAAGGCCTTAGAGATTTTAAAAACAGCCCAAGCTAGTGGTCAAGCTAACTTTGAGGTACAAGACTTTAACTTCGGTTTAGATCTCAAAACGGAACACGAACGCTTTTTGTGTGAACAGCATTTTCACAACCAACCGGTGTTTGTAATTAACTACCCCAAGGACCTTAAGGCCTTTTACATGAAGCAAAATGCTGATGGTAGAACTGTTGGTGCGGTTGACCTTTTATTTCCCCAAATTGGTGAGATCTGTGGTGGGAGTGAACGGGAAGGTAATCTAGAAAAACTAGTGGAACGTTGCCAAGCAATGCAAATTGATACCCAAACACTTAACTGGTATTTAGATATGCGTAAATGGGGTTACTTTGCCTCAGCTGGCTTTGGTTTAGGGTTTGATCGTTTACTAGCTTACATCTGTGGATTGGAAAACATCCGTGATGCGATCCCCTTTCCCCGTGCCCATGGTTCCATTAACTATTAAAACTTGACAGAAAAAGTTACCTAATTGGTTAACCATTTACCGCATTTTTATTGCTGTACCTACCATTATTTTTTTGGGATTAAATCACTTACTTGGTAGTGTTGCTAGTTTTACAGTGTTAGGCAATGTCACCATTCACCTACAGGTTAGCCTTTTTATTGGGGGAGTCTTGTTTATTACCGCAGTTATTTCTGATTATTTAGATGGGTATTGAGCGCGGAAGTGAAGGGTTGTGTCTAACTTCGGTAAGCTGTGAGATCCGTTAGCTGACAAGGTGATTATTAACGGTGTCTTAATTGTCCTAGTAGCATATGGCTACTTTCACTTTAGTTTTTTAATTGTGATTGTGCTGCGTGATTTAGTGCTAGACGGATTGCGCTTTTACGCTCAGGAAAAACAGCTAATTATTCCCGCCAACCAATGGGGTAAGTGAAAAACCACTTGACAAATGATAGCAATCTTAATGAGCTGTTTTGTGTTTAGTTTTTCTTTGAAGGAAACCAACAGTGCTAACACAAAAATCTTTTACTGAGCCATAGTGCATTTGCCCTATTATTTAGCTACAGCCTTCTCCTTGGTCTCGTTTGGTATTTACGCCCAACAAATATATAAAACCATTAAGGTTAAAGTAAAGTTATAATTTTGGAACTTTCTTATTGTTGTGTACGCGCGCTTAATTGCTGAAAAACTGCTAAATCATAAGCTCACCATTGCCACTGCAGAGTCAGTTACTGGGGGCCTTTTGAGTAGTAGCTTAACCGACATCGCTGGTGCTTCTCGTTTCTTTAAGGGTGCCATTGTGGCATACAGCAATGAATTGAAAAAATCACTTTTAAATGTCAAACAATCAACCTTAATTAACCACGGTGCTGTATCGCGTTATTGTGTTCGTGAAATGGCGTTAGGTTTAATGCAAAAATTCAATGTTGATATTGCCGTGGCCTGCAGTGGGTTAGCGGGTCCTGATGCATTGGAAAACCAAGCAGTTGGTAGCTTGTTTTTCTGTGTGATTGTTGCCAACAAGGCTTATGACTTTGAAACAAAACTACCAGCTGGCTCTAGAAACGAATTGCGCCAGCTTTTTGTGCAAAAAATCTTGCAAACAGTTGAACACATTTTAAGTGAAATTTCTTAGGTTAAATAAGGTTTAAGAGAACAAGATGCAGCGTACTTTAAATGTTGGGGTGATCCTGTGTGAAAGCTTTATTTCCAACGCCCAAAACCCAGTTAACTCCTACATTAAGATTTACGAAAACGTGAGGATGTTTGAGTTTGCGATTAAGCTACTTCAAGAATCGCGAATTAACTTCCAAAAGATCCTTTTATACGTTTTGGAAGAGCAAATTCCACTTGTGGAAAAAGTAGTTGCCAAGTACGAAAACTGCTGGGTCTTTCGTTCCAAGCACAACGAGGTTGAGGACATTTATGAAGCCAAGGGCTTTATTGAAGACAAGTACAAAATTGGTTTAAAAAGTTCTAAAAACCAAGCCTCTTCGTATTATGACAACTGTTGTTTTGTTGTCTTGGAAGCATGTCGACCTTTAACTTCCAAGAAGGTTGTTAAAAACGTGTATGAAAAGGCAATGATTGACGGTGCCGCTGTAGCTGTACTACCGTTTGAAAGGCAGCTTGTTTGTGGTGATAATACTAAGGCGGTGCGCCAGTTAAAGGACAAGGGCAACATGAACTATTGGCGTCAAAGTAGTACTTGGGAGTTGCAATTTCCCCAGGCTTATACGCTCAATAAGTTAAACCAGTATCATAAAAACCTCTTTATGAAGGCGCGGAACATGCTTGATTTAATGAACATTTCCGCTAAGAATCCCCTAAGCATTGTCGATGGTAGTGCTTACAGCTTTCGTGTAGTTACTAGCCTGGACTTTGAAATTTTGTTAGGTATTTTGCGCAATGGATAGAAAAATAGTTCAACTAATTCATAACTTTAGCGGTACAGAAAAACTAAAAGCAGCTGTTTTTTCACACGACGAACAGTGTTTCTTTGACTTTGTTAGCTTCGATGAATTAAACAATAAGCTAACTGGGTTTCTGTTATTTGACAGCTTGGAGAAGCTGTTCAAACTAGTAGAAACAATTCAGCAAAAGCGCAGTTGGCTTTATGTTGATGAATTGTGACTGATCAATACTGCAACTGACAACCAACAACTCAATGAAGTGAGCGTTTGGTTAGTGAAAAAGGAGCTTGCGCAAGTCGGTGTTTTAACCCAATTGGATACTAGTTTAGTCAAGCTTTTAATGGCTTCCAAAAATATTGACAGTGCCCTTTACAACACTTACATTAAACCAGTGGAGTTGCAACAGTTTACCCAAACACCAGCTCCTGATAACGTTAATGCAGAGCAATCGCATTTAACCTTGGAGTCTACCACCGACTTGAACAACAGTCAACTAGCCAATACTCCAGCGTTGTGGGAAGTGGAACAAACGACCCAGGAATTGTTACCAACCATGGACTTTTCTAAGTTTATTGATGAATTAGATCAAATTACCAAAAACTTTTCTGATTTAGAGTTAGAACCACTTAGTTTTAACGAAGGTTTTGATGAGTGAAACCAAGAGTAAAGTACTCGTTTTAGGCGGTTTAGGTTACATTGGGAGCTGTTTTATTGACCAGCTTCTAAAACAGTACCCTGATGTAACTGTTAGTGTAATTGACATTAACCACACTAGTCTGGCGCTACAACTGTTACCAAGACAGGTCAACGTTCACTTTGTTAACTTACTTGACCGTGCACAGTTAACAGATACGATTGCTCAAATTAACCCTGATGTCGTGTTTCACTTTGCGGCAAAAACAAGCGTAAAGGAATCGACCGAACAACCACTTACATATTTTGACCATAACCTAGTCGGTACGCTCAACTTACTCCACGCTTTAAAAGAACTACAAAAACCGATCCAGCTGTTCTTCTCCTCCACTGCTGCAGTCTTTGGTAGTGCTAGTACACTTCCAATTCCAGAAAACTTAGTTTTAGAGGAAACACTCGCTTCCAATCCGTATGGCATTAGTAAGTTTTTAAGTGAAATAGTACTGCAAACACTTACCCGTAGCCCCCATTTTCAAGTAATAGCGTTGCGCTACTTTAACGTAGCGGGTGCTAGTAATCCCTTTGGTAACTTCAATAAAAACACGACCTTACTGATTCCTAACCTGATTAAAGCCTTTATGGAAAAGCGCACCTTCTTTCTGTATGGTGATGACTATGACACTAAAGATGGGAGCTGTATCCGTGATTACATCCATGTAGTAGACTTGTGTGATGCCCATTTGTTGGCGTGAAAGTGGTTGCAAGCCAATCCTAAAGTGCGCTTTGAAAGCTTTAACTTAGGTAGTGGTCAGGGCTTTTCCAATTGGGAGGTCATTAATACTGCTCAAGCAATCTTTGCGCCTGAACAATTACAGCTCAAAATTGAATCACGAAGGGCTGGTGATCCACCTGTTTTAGTGGTTGATTGCACGAAGGCAAAACGCTTACTCAACTTCCAACCAACCCGCTCATTACACAAAATGCTGAGCGATGAAACAATTTTTTATCGGGATTTTTATAATAGGCTTTAAATTCAATTTTGGCAATGTCAAAAATAGCCTTCAGGATGGAAAATATCTGCAAGAGTTTTGACAATGGTAGGGTCAAAGCTAATGTAGATGTTAACCTCACGGTTTACGAAAATACTGTCCACACATTGCTGGGTGAAAATGGTGCGGGCAAATCTACTTTAACTTCCATTTTGTTCGGTTTATACCAACCCGACAGCGGCAAAATTTTTATTGGTGAAGAGGAAGTACACTTCAAGTCCTCCAAGGATGCCGTGCAGCACAAAATTGGGATGGTGCACCAGCACTTTAAGCTGGTTGATAACTACACCGTCTTAGACAACATTATTTTGGGCAATGAAAGTAGTTTTTCCATTCCTTTTACCAACGGTAAGCTTAAACTACCTTTATTGCACCGTAAAGCTAGCGAAGCCAAAATTCAAGCAATGATGGAACGGTATGACCTCCATGTGAACTTGCACCAAAAGGTAAGCCGCTTAACGGTGGGTCAGCAACAACGGGTCGAGATCTTGAAGGTGTTATTCCGTGACAGTGACATTCTCATCTTTGATGAACCAACCGCTGTTTTGAGCGACCAGGAGATCAAGAGCTTTTTAAACATCATTAAAAACTTCAAGAAGATGGGTAAAACGATTGTGCTCATCTCCCACAAACTCAACGAAATTAAGGAAGTAGCGGAGACTGCTACCATTTTGCGTCAAGGTCACTCCGTTGGCACCTTCCAAATTAAGGACACTTCAATTGATGAAATGGCACGCTTAATGATGGGAAAGGAGTTGAAGAAAACCAAAAACAATACTCAGTTTACCGCCAAAGGTGAGCCCGTTTTAAAGGTCGAAAACCTCCATCTTTATTTAAACCAAAACTGGTTTTACAAGTTAATTGCGCGCTGGAACCAAAAGCGAATTAATCAACTCCAAAAACAGGGTAAGCCAGCGAAAACACTGTGGTTAAAGAGTTGACTAGAAGGGTTGGCAGCTATTGAAAAAACACCCCGTTTTATTCGTGGAATCGTTAATAATTTAGGTTTTGGTAGCCAACAGGTGTTTGATAAAGGGATTAGTTTTGAGATCCATAAGGGTGAAATTTTTGCCATTGCCGGGGTGGAAGGTAACGGTCAAAACCAATTAATAGATCTTATTTGTGGCTTGGAAAAAGCAGCACCAAAAAAGGTCTTCTTCAATGGTTTTGACATTTCACGTTATTCCATTCGCAAGCGGATTAACGCTGGTATTGGTTTTGTGTTAGAAGACCGCCATAAATACGGATTAATCTTAGACCAAACGGTGCGTTTTAACGCGGTGAATAACCAAATTGATCGTAAGCAATTTAGCAGTTGAAATTTTTTAAATCAAATGCAAATAGCTGTTTACACTAACCAAATCGTCGATAAATTTGATGTTCGTGGAGCGGTACAAGGTACAGCAATAGTGCGTTTACTTTCCGGGGGTAATCAGCAAAAGCTGATTATTGGTCGGGAACTGACAAAGCAGAACGAGCTGTTAGTGTTTGCTCAAGTAACCCGGGGTTTGGATGTTGGTGCCATTTCGTTTATCCACCAAAAGATTTTGGATGCAAAGAAACAAAATAATGCAATCTTGTTAGTGTCATATGAGTTAGATGAAATTTTAGCTATTGCCGATACCATTGGCGTAATTAACAAAGGCAACCTGTTGGAAGTCAATAAACGTGATGTAATGACGCGTGAACGCATAGGCAAACTAATCATGCAATAAGAAAAAACTGGGAATTATGAATGCTTTAAATTACCTCAAACACCGTTTTCTGTTTAGTAAAGACAAGTTTTGGTACGCCCCTTTTAAACAAAAGCAACGTCGGAGCATCTACTCCACCTTTTCGTTGATTGTTCTCTCCTTTATTGTTTCGTTCTTTTTAATAGTCGCCATTCCCGGCATTAAAGGCGGTACCTTCTTAGAAATCTTTACCCGTTTATTTAAAGATAGGGTTAACATCGAAAACTTTGCACGACAAATTGCGATTTACACCCTAGCTGCTTTGGCGTTTAGCTTTTGTATGAGTGTTGGGGTGTTTAACATTGGGATTTCGGGACAAATGATGGCTGGCGCTAATTTTGGTTTCATGATGATCCTCAAGGTTTTCCCCGAGTCATTCCGTCCTGCCTTTGGTGGTCAGATCATTACCATCCTCTTAATGATTCTAGGTAGTGTGACCGTTGCTATGGTGGTAGCTGCTTTAAAGGTCTTCTTTAAGGTTAATGAAGTGGTTAGTGCCATTATGCTCAACTGGGTAATTGTGCTGGTTAGCGCTTATCTAGTGGGTACTTACATTAAACCGGAAAAAACTGATACTTCCCAGTTTTACTCGATTGAACTACCGGATGCCTTTGCGCTGTATAACTTCTCTGATGTTCAACAAAAGTATGGTTGATTAACCTCGTTGGTTATTGCCATAGCAGCTGCCATATTCATTGCAGTTTTAATGAAGTTCACCGTTTTTGGACACAAACTCAAGTCCACGGGTTTAAGTGTGACGGGTTCGCAAGCCGCTGGTTACAGTGTTAAGAAGTATCAGTTCCTCTCGTTTGTGATTTCAGGAATCCTTTCTGGTTTACTAGCAGCTGTAGTTTATACCGCATCCTTTGAAAAGCAGTTAACGTTCTCAGATGTGGGTGACTTTGGGATTACATCGGTACCGATTACTGGGTTTGATGGAATTGCAATTGGTTTAATTGCGCTCAACAGTCCAGCGCGAATTGTAATAGTTTCCACCATTATTTCATTTGTAACCATTGGTGCTAAACCAGCGGGGTTAAACGCTGCTACTGCGAGCTTGGTGTTGGGTATCATGATGTATTTCGCTGCCATTTATAACCTGATGATTTACATCAAGCCATGGCGCATGATTGTCAAGCTCAATATTGGTAAGATGAACGAAGCGGCATATGATGAATTCCAAAACGAGATGGCTGCTAATTTAGAAACGCTGTCGTTCCAGCGCTTTTTAGATAAACAAAAACGGAAGCATGATAAAGAGCGAATGGTTTGGTTTGACACCAAGCGCTTTGAGGAGTACCAGAAAAAGAAGCAAGCAACTCTACAAACCTTCCATGAAAATTCCTCGCAAAACTTACTGCAGTACTGAAAGCAACAGTTACTAGTAGCCGATGTAAAGCGATTAACTTTCAAATGAGACTTTCTTACCTTTAAACACCAGCAAAAGTACATCTTACGCTGGTACAAGGGCAAGAACAAAAAGCAAACAGCACTAGAAAATGAGTTTGCTAGTTTAAACGAAGCTATTAGTCAAAAATTGGAGGAGAAATAATGTCACAGTCTTTAATTTCTTTTAGTAACTTAGATAACTGGTTGTTTGTCGCTCCTGCACTGCTACTCGCTGTATTGAGTGGTTACTTATCTGAGCGGGTTGGTATTGTCAATATTGCCATTAACGGGGGCATGGTGTTTGGCGGGATGTTCTTATCCTTAATGTCTTATGCCTTTGTGCCCAATGCAAATGATTCTGCACCATCTTGATCACTAGCAATAAGCATCCCTTTAAGTGTTATCTTTGCCTCAGCTGTTGGTTTTCTGTTTGGCATTGCCGCCATTAAACTCAAAGCCGATCACGTGATTGTTGGCACAGGGGTTAACTTACTGGGCACGGGAATTAATTTCTTTGTGGCACAAAATGCCCGCTCATTGCTTAACGATACTGATTTAAGGGTACGATACAGCTTTGTAAGAACAGGAAACTCTGTAAGTATTGAGGGTATAGCAATCTTTGCCTTTGCAATTATCTTTGTTTTATTAGTGTGGTATTTGATGAACTTTACTAAAACGGGCTTGCGTTACCGTGCTGTTGGGGAAAATCCTAATGTGATTGATACCCAAGGTATTAGTGTGTATAAATACCAGTGGTTTGGGGTAATGGCTTCGACGATGGTAGCAGCTTTAGCGGGTTGTTGTTTTGCTTTAAGTCCACAGGTACCTAGCTTTTCTAGCGGGGATGTGAGCGGGTTTGGTTTTATTGCCATTGCCATTATGATCATTTCGATGTGACGAATTATCCCAAGTATTGTGATCTCGCCCTTGTTTGCACTAGCTTATGTGCTTACTACCGGTGTGGTAGGTAATGCTAATAACACTTACTTATTGAGAACTATTCCGTTTATTATTTCGCTGTTGGTAATGATGGTATTTGGATATCTCAATGTAGGGCCGAAAAACGTTGGTAAGCATTTCGATAAGGGTTTGCGATAGGTTAACCTTTATTTATAGTTATAGTCAAGCCAACAAATGAGTAAAAATCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACGTTGCAAAAATATTTACCGAATGACTTTGAAATAGTCTCTACCATTGGTCATATTCGGGAAATGGTGCACAAAAACTTTGGTTTTAACGAAGCCGATTACTCACCAGTTTGGGAAGACTGAACTAAATCAAAAAAGAAGTTTTCTAGCCTTAGCTTTAAAGGTAATCTTAAGGGCAAAAAACTTTTAAGTAAGTACGACATCATCAAAAGCATTAAAGAGAAGGCTAGTAAAGCCACTAACATTTACCTAGCAACCGACCCAGACCGTGAGGGAGAGGCAATTTCCTGACATGTTTACGATGTTTTGGATGAAAAGGACAAGTCGAAGTGCCAACGCATTACCTTTAACGAAATTACCAAGAACGCCGTTTTAGATGCACTGAAAAACCCACGGGAAATTGATCAGAGTTGGGTGCAGAGTCAGTTTGCGCGCCAAATTCTTGACCGGATGATTGGTTTTCGCCTTTCAAGGCTGTTAAACAATTACCTCTCAGCTAAGTCAGCGGGACGGGTACAATCAGTAGCACTGCGTTTTTTAGAAGAACGTGAACAGGAAATTCGTAGCTTTGTACCACGGTTTTGGTGAACGCTCGATGTGTTGTTAAATCCCAAGGCTGAAGGTGTTAGGGAGGCGTGTGCTAATCGTTCAATTCCAATTGTCTTGCGTGAAATTAATCCTGCTTTACGTGCTGGCTTGAAATTTGAAGAGGAGAAGAGCGTATCGGGAATTGACTTTTTAGATGAAGCATCAGCTAAAAAGTTTGGTGAACAGCTCAAGGGTGTCTTTGAGGTGTACAACATTGATGAAACCAAACACTACAGCAGTTCGCCCAATTCCGCTTACACCACCGCTTCATTGCAAAAGGATGCCATCAACAAACTAGGTTGGTCTTCCAAAAAGGTAACCTTAATTGCCCAACACCTGTATGAAGGGGTGAGTATTAATGGAGAACAAACCGCTTTAATTAGCTATCCCCGTACCGACTCAACTCGTTTATCAGCGCAATTTCAACAAAGTTGTAAAGAATACATCTTAAACCATTACGGAGAAAAGTACCTCTCTAATCGGATTGTAAGCGCTAAGGGAAAAAAAGGTGAAAAAATTATCCAAGACGCCCATGAAGCAATTCACCCAACTGACATGAACATTACCCCGGAAATGGTCAAGAATGCGATTAAGAAGGATGAGTTCTTACTGTACCGCTTAATCTGGATCCGTACGGTCGCTAGTTTAATGGCGGATTGTAAAAAATCACATACCCACATCCGGTTTATTAACGATGGTAATAAGTTTTACGCTTCTTCCAAGTCATTAGTATTTGATGGTTATCGCAAAATTTACGAACACTTTGAAAATAAGGAAAGTAATGATCTCTACATTGATTTGGATAAGATTAGGGTTGGTGATCGTTTCATGGCTAAAGACATTAAGATCACAGCCCGCCAAACCCATCCAGCAGCCCGTTATACCCAAGCTAGTTTAATTGAAGCGTTAGAAAAGTCCAATATTGGTCGACCTTCCACCTATAACACGATGGCTAGTGTTAATTTAGACCGGGGTTATGCTAGTTTAAATAAACATGCTTTTCACGTAACACAACTAGGGGAGCAGGTTAATGAAGAATTGTCCAAACACTTTGGTAAGATCATTAACAAAGAGTTCACTAAAAACATGGAGAAGTCTTTGGATGAAATTGCTGAAAATAAAAAGAACTACCAGGAGTTTCTCCGTGATTTTTGGTCAAACTTTAAGGAAGAGGTAAAGCTAGCGGAGGGTTCGATTCAAAGAGTGAAAAAGGAAAAGGAATTTGTTGGTCGTGATTGTCCAAGCTGTGCTAGTCCCTTGTTGTACCGCTATACTAAACGGGGTAATGAAAAGTTTGTTGGGTGCAGTAAATTTCCCAACTGTAAATACAACGAGTTTAGCCAAAATAAACCGAATTTAACTTTGGAAAAGCTCGAAGAACTGTGTCCGGAGTGTAACAGCCAACTAGTGAAAAGACGCACTAAGTTTAATCCCAATAAAACCTTTGTTGGTTGTAGTAATTTCCCTAGGTGCCGCTACATAAAGAAGGATAATGCTAGCTAGTTTAACGAGCAGAAGTGCCACTTCCTTCTCAATAATTTGAGCGTTGGTAAGTGCCATTTTGATCTTAAGTATCCTCATTTGGCTTATTATCACCATTTTCTTTGCTTGAAATTTACACCTCAAAAACAACAAGAAACGAACAAAGTACCACTTAGAGCCGGAACAAATTAAGCACAAGATCATCCAAAATAAGACTAAATTGGGCAAAATGTTGGACTTTTACCAACAGCAGATTAACACTACTGCAACTGAGCTGAAGTGGTTGGATGGTCAGTTTCAGCAAATTGATGAAACCGATAAAAAGAAAGCGCATCAAATTACAATTAGACTAGCGCGGAACCAACTGTTACAACAGCTTAGTGTAAAGTTAGACCAAAAGCAGTTTAGTCAACGCGCTAATAATGAACTGCAAAAGTTAAAGTTGTCTAACTTAGAGAGCTTTACTGACCAAAAGATTAAATGAGACCAGGAGGGGATGAAATCCGCTGTATCCCGCGTTACCATTAACGAGTGAACCTTTAACCATTTTGCTGGTAAAAACCGTGTTTACTGGGATTACTTTAAACAAGTTTGTGATGTGGATTGCTCGATTAAACCACTTAAAGATCAGTTAGAGATTACTTTTTCGAGCTGAAGCTTATTGAAAAGACTGCAAGCAAAAAACCTATTTAATAAGTTAATTGCTCAAAGTAGCAGTGTCAAGATGAGCGAAAAACTGATTAACAATGCTTTGCAATTAGTTCAGGACAATTTGGCATTACAAGCGAGTGAAAGTGGCAATAAGCTGCTCAAGGAGTTTGAATTATCGTGCACTAACACACAGCTAGTCCAACTGTTGGGCTTTCAGCAGTTTTACTTCGGTACAAACCTGCTTAGCTTACTTGATTTGAGCCGTTCAATCGCTGTTTTAGTGCGCTTTTTAAATGAGCACTGTAAGTGAGAATTAAACGAACGCTTACTAGTGGAAACTGCTTTGTTTAACAACTTGCAATGGGTTAATAACAACGACTTCTTTTTAAAGTCACACAATGATTTAAAACAGCTGCACCTCTCCGCTGAACAGTTGGCGATTATTGAACAGCAAAACCGTCCCTTTTACATTGATGCTTATGCTTTATTGATTGCTGGCGTAAAGCAAATGCTGATGGAGCACGATGCTGTGGAACCAAAACAAATTCACTTTCATAATGCCAAAAAGGTAATGGAAAGCTTCCAGCTGTTTGGCATTGACCAGTTAGCGTTAATAGAGTACAACAACTGTCTCTATGGCTTTGTCACCACTAAACTCTATGAGATTAAACAATTAGATGATTTAGCTTTATTTAAAGTTTTGTTTAAAAGCTTTTTGAACAAGCATTTGAAACAAAAATTTGCAACAATCAGTTTATTTGTAAATACCCAAACACTCATGATCTAACACTTTAGATCATGGAAACAATTAGCGAACTTTTAGAATATAAAACAATTGAAAACAAATAGTTTATGGTAACTGAAATTAAAAGTCTCAAACAACTCGGAGAGTTATTTGCTAGCAACAATAAGGTAATTATTGATTTTTGAGCAGAATGGTGTGGGCCGTGCAAAATTACTGGACCAGAATTTGCTAAAGCTGCTAGTGAAGTGAGCACTGTAGCGTTTGCCAAGGTAAATGTCGATGAACAAACTGACATTGCCGCTGCTTACAAAATTACCTCACTACCTACAATTGTTTTATTTGAAAAAGGCCAGGAAAAGCACCGTGCTATTGGTTTTATGCCTAAAGCCAAGATCGTGCAACTTGTCAGCCAATAAAACTTCGTTTCATGATTGACTTGCTCGGGTTAGATCTTGATGGCACTTTGTTGTCTAGAACAAGGCAAATTAACGATCCTACTAAACAAGCTTTGGCTAATTTAATCCAAAAGAAGCCTAGCTTAAAGGTAATGATTCTAACCGGTAGATCATTATTCTCCACCTTGAAGTACGTTCAAGAGCTCAACGAGCTGTGCAAAAAACCGCTAGTGGAGTACTTTTGCTGTTATGGTGGGGCTAAGTTGTACCAACTCAATAACAACCAACCCCAAGAACAGTACAAGTTTTTAATTGACAGCCGGCAAGTTAAAACAGTGTTTGAAATTGTGGAACAACACAAGGGTTTGTTTTTGGCATACCTCGATAAACCAAAGGCACCCTACATTATTTTGGGGGCAAACCAGTTTTATGCTTGACTGATTAAACAGTTTTGGTACAAACAGCGTTGTGAGTACTTTAAAAATGATCATTTAACCGATGGCATCTTAAAGATTAATGTGTACTTCGCGTGTCCTTTAAGGTTGAAGAAGGTGTACCAGATCATTAAGCGTCAGTTTCAAGACACGCTTAACGTTGTGAGTTTTTCCAAGCACTTAATTGAAATTACCCACAAGGATGGAAATAAGGGCTATGCAATTGAAGCAATTGCCAAAAAACAAGGACTTTCGTTAAAACGGATGGCGGTGATTGGTGATTCCTTAAATGACCGTTCGATGTTTGAAAAGGTACAGTATTCGTTTGCAATGTCGAAATCACCCGATGAACTCAAACTGTTAGCCACTGAAATTGGCACTAAAACAAACCGCTTTCGTTTCAGTAGTTTAGTTGATTTAATTACCGAAAAAATTATTAACTAAAACTTTTTTACTTGATGTTTTGGCTTCCTAGTCCAAAGCCATCATAGAAAAGTGCTAGTGTTGTTCTGGCCGTTGCTTGGGCTTGATTTTTACCCGCCTGGAGGCAGTTAAAGACCTGTTCACGACTAATCTGTTCCCGTTTTCTCTGAATGTTAACTAGCAGATTAACGGTTGCTTCTGTAAGTGCGTTTTTGAGATCTAAATAAGATTTAGTACTAAAGTAAGCTTCTAGATCATTCCCTAACTGATTGGTTAAGGATTGATTGACAGGCTCTTTTAAAAGTGCCTTGAGAATTGTGAGCATATTGGTCACCCCTGGCTGCGTTTTGGAAGCAAAACGGATTTTGTTAAAGCTGTCAGTGGTAGCTTGACGAATTTTTTTAACCACTACTTCTTTTGGGTCATCCAAGTAAATTACTCCGTTTTGGTTCTTTGAGGACTTACTCATCTTTTTGGTTGGGTCAAATAAGTCCATAATACGGTTGGTATCTTTGTTTTGGACAAACTGGGGTAGACGCAGCTTGAGCTTAAACTTCTTTTGAATTCGCTGCGCTAAATCACGAGTAAGCTCTAGGTGTTGTTTTTGGTCATTACCAACGGGCACAATGTCAGGTTGGTACAGCAAGATATCTCCCGCCATTAGGGCTGGATAAGTTAACAGTCCGGTGGGAATGTTTAAGGTGCCGTTTGGGTTACGGGTTTGTTCCGCTTTTTTGGCCTTGAACTGCGTCATCCGTTGCAGTTCACCGAGATTGCTTTGCACCATCATTAAATAACCCATCATACTGTGCTCCAAAAGATCGCTTTGTAAAAAGAGACAAGCTTTTTGTGGATCTAGACCAACCGCTAATAAAGTACGTACCAAATCAAAGTTATTTTGTTTCAGTGCTTGGGGTTGAAAATCAACGGTAATTGAGTGTAAATCAGCGACAAATACAAACAGTTGGCATTGCTCTTGGAGTTCAATTAACGACTGCATTACCCCCAAATAGTTGCCTAAATGCTGTTTGCCCGATGCTTGAATCCCGGTAAGAGCGCGTTTCATCATATGATTGCAATTTGTAGAATCTATTACAGATAGACAAACGTTATTTTAAAAGAAAAATGCTTAAGAAAAAAGTTAATAATGATGCTGGTAAGACGTTTATCTTAATTTCATCATCTTGCTCGTCTTGCCAAAAGGCAATCGAATACTTTAAGGAAAATAATTTGAGTTATACGATCGAAAACTTTTACAAAAAACCGATAAGTGACAAGCGCTTTCGTGACATTTTAAGTTTGAGTGAAGATGGCACCGAAAGTCTGTTTTCCAAGCGTGCTGACCAAATTCGTAGTAACACTAATCAATCAGTGGAAGATTTGACCATTCCTGAATTGATTAAGTTAATTCGCGAACGTCCTTCAATTTTGCGTCGACCAATTATTATCCAGTACAACTCTTCGGGGATTCCCAAGCGGATGCGCATTGGTTATAACGCCGCTGAAATTAAGGTATTTGAACGCAGCTTAATGGAACCTAAAGTGCGTACAGTAAAGAAGTAATGAAGTACAAGATTTTTGCATCAACAACACCACAAACCGAGCCAGTTTTGCAAAAGTTAAAGCAGGTGTTGAAAGGTTGTGAGGCAGTTGAAAAGGGCTTTGATTATCTCTTTGTATTAGGTGGTGATGGTTTCTTTGTTTCTACTGTGGCCAACTATAACTGCCACAATTGTAGGGTAGTAGGGATTAACACTGGTCATCTAGGTTTTTACACATCGTTTAATGAAAAGGATTTGGATGATAATTTCCTCCAAAAGCTCCAGCAATGTCACTTTCAACGGATTAGTTTGTTGGAAGTTAGTGTCAATGGCCAGCAGCACTTGGTTTTGAATGAACTGGCAGTTTACACCAACACAGCTTACCCAATTAACATCTTTATTGATGGTGAAGCGTGGGAGTTTTACCGCGGTTCAGGTTTATTAATCGGTCCGCGTACTGGTTCGACTGCACTGGCTAAATCTGCTAAAGGGGCGGTGATTTTCCCTGGCATTGATGTGCTGCAAATTATTGAAATGAATCCACTTTTGCACCCCAATCAAGTTACTATCCAATCACCAATTATTTTGCCCAAAGAAACGCAAGTGGAATTTGTGGTCAAAAAAGCCTTTAACCCACAACAGTTTCCCACCTTTTACTGTGACGGGAGAAAGTTGGAATTGCCAAACGCTGACACAACTTTAGCGCTTAAACTAGTGCAATCCACACCGATGTTTAACATTTCACTCAAAACACAGGACTTTATCAATAAACTTAAGTCCACTTTTATTAAACAAAGCTAATGAAAGTATTGTTGTGAATTGGTTATGTGTTGAGCTTTGGCTTACTTTACCTTTATTTGGTAAAACGTGCTAAGAGGGCCGCGCTACAATTAAACAACAAACTAGTCGAATCACACACAATTCCTTTTGCTGTACGTGACTTTATTGCCGCTTGCGGGGGTAGAACTAACTTTGTTAGTTTACGCACCTCACCCACCCAATTAATTGTTAGTTTTGCCAAGCCAGAGTTAGTTCAAATTGCCGCTTTGCAAAAGCTTGGAATTAAAGGGATTAATAAGAGTCAGAACCAGTACCGCTTTGTATTGGGCAATTTTGTCAATCAATTAAAACAGCAAATTGAAAATGAGCGCTAAGTTAACGTTAGAAAGCATTGCAAAAACATTTGCCGAAACATCGATCTTTGCGATCTTGTTTTTAATTATTGTCATCCTCAATTTAGGTTCACTTGTTTTTTTAGCGTATCAGTACCGTGTTTACAAAAAGAAGCAAAGAGCAAACCTCACCAAGCAGACTTATAACAATGACTATGGCCAAATTGTTAATTTAAAACAGCAAAACGCTGCCAAGATAAAGGAGCTTGCTAACTTAAAAGATCAGTTAAGTGAATTAGGCCAAAAATTTAACACCACACTGAGTGAAATCATTAACAAACCACTTGTCAATGTGATTGATGAATACTTGGATGAACAGTTTAAACAAGCTGCTAATTTTCGTGAAGCAGAACTTAATGCCGTTTTAGATAGTAATGACCAGAAAACAGTGTTCCATAAGCGTTTGTTCAATAAATTTCACTTTGGTGTCGACAAGCTAGCTAATATTAATGTCAAAAATCCGCTCAATTTATGCTGGGTTGACAGCGCTAGTTTTACCGTGATTGAAAGTGATTTCCGCAAACTTAATGGTGTTGGCGGGATTAATAAAAAGTTGTTAATTGAAAAGTTAAGGATTGAAGATATTATCTTTACCAATATCGATAAGAAGTACTATGAGGTACAGATACTATCTGACTCGCCAGTAAAGGTACAAAAAACAGTGTTAACTATTCGCAACATCTTGATTAATGATTACGTGGACAATGAAAAAATTGAGTCATATGCTAGGGAAGCTAACGGTTACTTCAATGACCACTGTAAACTGATTGGTAAGCAGGTGTTAGAACGACTCAATATCTTTGAAATTAGCCCTAAACTACACAAGTTTTTTGGTTTATTAGCGTTTCGTTATTCTTTCGGTCAAAACGTTTTATCACACTGTCTGGAAACTGGTTTTCTCACCGCTTACCTAGCGCTTTTAGTTAACTTTAAACCTGATGTTGCATTAAAGTGCGGTTTGTATCATGATATTGGTAAAGCGGATGATGAAAACGGGAAGAAGAACCACACGGTTACAGGTGCCAAGATCGGTGATGAATTTTACTTTGAAAACGATGTCAAATACACAATTGCCAACCACCATAATAAAAATGTCGATAACGTATACTGTCGTTTAACTCAAATTGGCGATAAGCTTTCAGCAGGGCGTTTGGGTGCGCGCTCTGACAGTTCGGTTTTATTTAACCAACTCAAACAAGAATTAAAGCAAATTGTGGAAGAAACACTAGCACAATTTAAAACAACCATCCTTTTAGGACAAAGTGGACGCCGATTAGTAATTTGATTGGAAACCAACCAGCACAATAACATTATTGACAACCAACAGTTAACTGATTTGGCTACTACCATTAAAAGCAAAATCGTGCAAAACAACATTACTAATCGCTTTCCAATTAAGGTAGTATTACGCTATAACTTCGAACACAGCTTTGATACAAAGGACAAGAACTAGTTATGCAATACACCGTTTTAATTCCACTGTTTATTTTTATAGGAGCCATGGTGTTGTTTGGTTTTAGCTTTCAGAAAAAGCAACCACAACGCCGCATCGTTCAAATTCTGTTCTTAGCTTACTGTGTGGATTTTTTAGCACTAATTATTGCAGTGATGATGCTCACATTCTTAAGTTATGATGACTTAATGCTGGGCGTTTTAATTCCAGTATTAATCCTTTCAATCATTATGTTCTTTGTGATGATTATTGCCCACTATCCACTAATGAAACGCTTGTTTGGACATTAGACAAAACGTGTCTCATTCAAGTTGTGCACAAACTTAGGTGTTTTAAGTAGATAGTCGAAGCGTTAAAAAAGTATTTTGGGTCTTCTACTTTTTCCCTAAGTAGATGTTTTTTATTGATGTAAATAAAAGGCCAAATTTAACTCTTATGAGTTAAAAAGTTCATTTTTTAAATAACATTAAACCGGAAATTTCAATTAGTTTCCTCTTCTTCAAAAGGATTTTCCAAAAAAAGCAAAATAATGCAATTATTTTTTACTGTTAATGCGTAAAAAGAAGTTTTTGTCAAGGTTTTCTTTTAGTTCTCTATTTTTATTATGTGGAACATTGTTAAGTGCTTGCACTGGTATCCAAGCTGATCTTAGAAATCTTATTAAAGAAACCACTGGTAAAGACATTGATTTGTCTAAAGCAATTAAAACTAAAGAAGGCAAAAAGAACATAATAGCTTCTTTAAAAAATCTTACGAAGTTAATCCGAAAGATACAACAAAATTGTTGTTAGACGCCTGAAAACAGAGTTTTGAAGAGGGTAAATTAGGTATTGCGGATTTTGATTTTGACCATGTAGCTTATCCTCAAACAAATGATCCTTTCACGATGGAGCGAAAGGTTGATCATTTTCAAATGACATACCAAAGTTTTAAGGATTTGTTAGTTGAGGCTAGGCTGAGCTATACCTTTAATTGATTTGGGGATTATTCTTCAGGTGATTTCACTGCTAAGAGAGGTGACAAGCATTATTTTTATCTTTTCCTTAAAATTAAATCTGATCCAAAAAAACAATTTTCAGCAAAAAAATTTCTTACTGAAGGAGAAGCGTTTACTGATCAAGAAGGAAAACAAACTACAAGAAATTTAGAGTGAATTGAGTTTAGCGCTTCGATTAGCTGATGAACAAAAGGCAAAGATGATGTTAGTCAAAAAAGTTTGAAGAAATTTTTAGAAAGCTTTGCTACTAACACAGGCTACTCATCAGATATTAATCTCTTTAGTTATTTGGAATATTTAATTAAGTAATTTGTTGTGATTTTTTAGTTTTACTTGGAAAAAATTTAGCCACCTGCTTTTCAACTTGAACAAATGGTTTTTTATTTGTACCGCATGAATAAAAAACCAGCAACTAACTTTTACAGATTAATTGCTGGTATTCTTTATTTTGAATAATATCTACTTAGAATCTAGTTTATCTAGGCGAACATCCATTGAATCCAAGCGATTGCTCATCTTTTGCAACATTTGCAAGATTAACTGTAGAGTTTCTCCTTGAACTTTTTGCTCCGCTTGAAGTTCTTTAATTTGTTCCCCTTGAGTTTGAACAGTTTCGGTTAATTGTTTAATTTGCTCACCCTGAACCTTGATCTGTTCACCCTACGTTTTTTGCAGTTCAAACACAACATCAACTTTACCTTCAACGCGCTTCAATCGGCTTTAGTGGCGAAGTCTTTGAGCTTTTCGTCAAGCTATTGGTGAATTACAAATTCATATGTTTTTGACACCTTTTTACTTCCTGAATTAGGATCATTAGGACCTTTTGGAAATCCTGGTTATTTAGGGTTCTCAGGTTATTCTGGTTTTTCTAGAAATTTTGGCAACTTCGGAGACTTTGGAATATCCGGTGTATTCGGATTCTCAGGTTTATCCAGTTGATCTTCTATTATCGTAAAGTCTTTATCCTTAAAATAGTACTCTTAGGTAGCTTGTGCAAACCATTTCTTTGCTTGGTATAGACAATTTGAAAATTAACTCTTTCCTTTGTCCGGGAAAGGATATTAAATTCCTTTTAGGAAATAACTTTAGGCATTAATAAATTCCTCCTTTCTGTTCAAGGTTTAGAAAACTAAAACGTGCAACTTTGTTGCCAGCAAGGATCAACATCATCGTTGATCTAATAAATATATTGAGTAAAGGCATAATTTCCCTTCACTAAATAAATTGAACGCCTTATTTTGAAATGACAACAAATTGACAAATTTCTTTTTAATTAAAGAAAATTTTGGAAATTAAGAGCTAATGCTTAATTTAAAGTCGTTGAATTTAAGCCTTCTTTTTGTTTTTGAACTCAATCGAATAAATAGTTTTGGTAATAATCCAATTGCTTTTTACGCATTTCAATTTCTTTGGGAATACTTTCGGTAAGATCGTTACATTTTTTTCAAAAGCAAAGAGGATATCGGCAATCTTTTCTTGAATTTCTAGAGGTGGGAAAGTAAGTTCAATTTCTGCTAATTTACTTATTCCTAATTTAGGAATAGGGCAAGCATAATTAACAAACTTAGATGTTTGCAATCTGAGTGCATAAGCTAAAAATTTAGGATTAATTTCTTCGTTTTTTACTTTTAAAACGCCACAGTCTTGTCTTGCATTAAACTGTCCTTTTCTATAGAATACAGCACCAGCATAAGAACCTCCTGTAGTTCAAGTCACGTACTCGCCATCTCGGTCACAATCTTTAATTTTGCCAACTTCACCATCATTTAAGGTTGAAGCTGAATAAACCGGGTATTAACCGAGATTATCCTTAATATATTTTTTAGTAATTGCACGACCCCTCGAGATCTCGCAAATATCTTTGATTTTGTAAGTTTTGATTTGCACAACAGTAACAGTTTAATTTCTTTTAAAAGTCATTTTCAGCGACTGTTTTAGATTTTTCTTTGTCATCATTATTGCGCTCTTTCTGCATATTAAGTGCTTGTTGGCAAATTAGAATAATACCCAGTTATGAACCGTCCTACTCCCAATTTTGAAGCAATCGATAAGAAGATTAGTGCCTTTGTCACTAACCACGATAACTTATTGGATAAGTTACTCAAACAACAAACCGAATTGTTAACTAGTGAAATTACGACTAATTTTGAGGTAACCCAACAAATCCAGGAAGAAGTAGCCAAAAAGACGAAGCAACACTCTAAAAACTACAAGTGACTAGCCACTGTTGTTTTAGCGAACGGTGTTGTAAGTCTCTTTCTTTTAGGTGGGTTAATCTACCTCTTTAGTAAGTAGTTCCCAATTAGCTTCAATTGAGCGGTTGTTCGTCTTTTTAACGTTGTAACAAAAGCCTAAACCAGTTTCGTATTTTGGTACTACATGCATGTGGTAGTGAAATACTTCCTGCCCTGCCTTAGCCCCTTCATTGACAACATAGTTTAATCCAGCTGGTTGGAGCCGTTGTTGTAGCTTTAAAGCCATTTCTTTGGCGAGTAAACTAACCGCCTTCAAACTCTCATCATCAGTCGAGGAATAGTTAACGGCGTGTTTTTTGGGAATTACAAGCGTATGACCATCAGCAACTGGAAAGGCATCTAAAAAGGCAACAGCGTGTTCGTTTTCACCAACCTTAAAGGATTTCACATTCCCCTCTACAATTCCACAGAAGATACAGTTATTGTTTGCCATACTTTGTTTTTGCACCATAATAACGGTTATTTATAACAAAAAGCCCCCACAGGATTAAAAACATTGATGCAAAAATAGGGATTGATCAAAACCAAACATAGCTGTTATATTTGGTTTGGGAGAAGAAACCATAGACCGAGTACGAATCATGCCCGTTGTCCCATATCTTTAGAAAAGGTACTGTTTGTAAATAAAAGGCATAAAGGCTTGGGTACACCCCTGCCTTGAGTAAAAACTTACCCAATTCATGTAGCGGCTCGCGCATTTTGTAAGTTTTAAAGAAGATTCAGATAAAGCCAAAGAAGCTAGCGGGGTAAATCACGTGGAGAAACAGCTGCGCAAATGAAATTCAATCAGTTAAAGGATTACCACTAAAACTGTATGGCATTTCACTCGTAATAGTCGAAAACCAACCCTTAACGTGTTCTTCTACATTAACAAATCCAGTAACACGTGACAGTACATACAGGTTAAAAAAGATGACGGTAAAGACCAAGTAACCTGCAGCACTTAAAAACAACGCATTATTGTCAAACAGCTTACAGCGTCGTTTACAGAGGTACATTAAGACACAAACAGCGGCAATTGTGTTGCTCTGGTAGGTAAAGGTATCAAAGTTAGTAAACCAGATTAATTGTGGTTGGTTGTTATAAACCACACTGATAACAGCACTAGCTACCATTACCCCAAGGTTAAACAGGGAAAACGGCGCGAGAAAAAGTAAATAAAAGTCGCAATTATTGTTAAGATAGAAAAAACGGTAAGCGAGTCCTATTGTTTTTTTCATCGCAAGTTAGTTAACTAATACATGAGTTTTAAAAAGATTACCGAAATGATGCGCCAAGCTGAACGACAAAGCAAGCAAAAAGCGCTTGATTTCGAGCAAAAATTGTTTGAGTACAGCTATAAGAATGCTGCCATCAAGATTATCATTTTTGGTAATCTCACCATTAAGTCTATCACAATCGATCCCGCATTAATTGACCCAGAAGACAAGGTCACTTTAGAGGAGATGATTACAGAAGCTGTCAATGAGGCTGTGGGTGATGTTAAGGCCAAGTACGACCAGTTAATGGAAGAGGCTATGCCACAGATGCCTGGGCTTTTTTAAGCTGCAGTGCTAGCTTAACCATTTGTTTATCTTTAGTGAGCTGTATAAAGGTTGGAATGTTGACGAGAACTGTCAACAATACAATGGCGAAATAGCTCAACCCAAAGCTAACTCCAAAGATTAAATCAGCATTTTTTGGATCTAGCTTGAGGAACACCGCTACATTCTTGTACTCACCGCTCAGTCAATCTTCAGCCAAAATGCCGGTAGTATTACCGGTAATTTGTCCCTTAAAGATGTTAACCATCAAGGCCACATAACTGAGATATGCTAGCGCAAACACGTGGATGTAGAAGAACGAAATTAAGCTAAAGCCATCGTCTCATAAAAAGACAGCCAGACTCAACAAGAAACTCAAGAAGTGCATCATGAAGTAAACCTGGTTGTTTTCCAAACCAACAAAGAGAAATTTGACAATCTCCTCTTGTTTTAATGGAGTAAAAATTAACTCGCCAAATAAGGTTATTGCAGCACCGTAAAAACCAAAGATCGCCAACACACCCGCCACCTTTTTCTGGCGTAAAAAGATAAAGATCGGGCCGATTAAGGCAAAGAAGGGGCAGAGATCTAACAAAAAGATCCTGCTCAACCTTAGTGAATCTAAAGGAACATTTGCAACAGCATTCTTAGCCTTGGCTGCAGTGCCGTAAATATCGGGAAAAATATAGATAATTTGCCGCCCGAAAAAGACATAGAGAAAGAGAATAGCTGCAAAAACTTGTAAACAATAAAACTGGATTTTTAATTTGCGAATTAATGGGGTAAATCCCAAGATAACTAGCACCCCGGCTACTGTTAGGATGACTACAAGATAGTTAAGCGACTGCATTAATTGCTTAAAATTAAGATAATAATTTTTTAAATTTGTCTTCGATGAGCGATCGTTTAAATGATCAAGCACAAAATCGCTTGCAAAAACTCCTAAACCTCAAACAAACTGGCAACGATCCCTACCTCATTACTAAGATAGAAAACACCCACTCAGCGGCTAGTTTTCAAAAAGCATTTGCCAACCAATCTGACGCTGAGTTAAAGCAGTACCAAGTGATCTTAACCGGTCGGATTATTGCGCTCCGGCAGACCTTTATTATTATCCAAGACTTTAGTGGGAAAATGCAGTTGTACATTAATAAAAAAACTGCCCCCGAACTGTTTGAATATTTCAATAATTACATTGATTTGGGTGATCAAATAGTAGCCACTGGCCATCCAATGATGACCAAAACAGGTGTTTTAACGCTGAACGTAGAGCGATTACAAATTGTCGCTAAATGCTTACAAACCCCACCTGAAAAATGACATGGGTTGACCGATCCAGAAGCGCGAGCACGAAAGCGTTACTTGGATTTGACCTACAACCGTGCGCAGGTCGAAGTCTTTTTGAAACGCACCCAGATTATTACAGCTATCCGTACCTTTTTAAATGAAGCGGGCTTTCTAGAAGTGGAAACACCCATTTTACAAGCCGTTTTAGGGGGTGCTAACGCCAAGCCCTTTAAAACACACTATAACGCCTTAAAGGGTGATTTTTACTTGCGGATTGCAAATGAAATAGCACTCAAAAAGCTCATTGTGGGCGGTTTGCCGAAAGTGTATGAAATGGGGCGGATGTTTCGTAACGAAGGGGTGGATACTACCCATAATCCGGAGTTTACGAGCATTGAAATGTATCAGGCTAACGCTGACTATGCGGTCATGATGGATTTAACCGAAAACCTCATTAAGTTTGTTTGCAAAACACTCAATCAGTGAAGTTTTAACTGGAACGGGCAAACACTTGATTTAGCCAAACCGTTCAAGAAAGTCAAAATGGTAGATTTAATCTGCCAAGTTATTGGGGTTAATTTTGATGATGTTAAGGATGATCAAACAGCCATTGCGCTTGCAAAGCAGCACAAAGTAGAGTTAAAAAAGCACGAGCAGAACAAACAACACATTATTAACCGTTTCTTTGAACAGTTCTGTGAGCATACCTTAATCCAACCCACCTTTGTCACACACTATCCCAAGGCCGTTTCCCCGCTAGCTAAACAAGATCCCCACAACCCCGAGTTTACTGAGCGCTTTGAACTTTTTATTAATACAAAAGAGTTAGCGAACGCTTACAGTGAGTTGAATGATCCCCTAGAACAACGTGCGCGCTTTGAACAACAGTTGCAGGAAAAACGCATGGGTAATGATGAAGCGAGTGAATTAGATGAATCCTTTTTGGATGCTTTGAGTTTTGGTCTCCCGCCAACAGGCGGCCTTGGGATTGGGGTTGATCGGTTAGTGATGTTATTGTGCGAGTGCAGTTCCATCCGTGACGTTGTTTTCTTTCCCCAACTACGTGAATTGAAATAAGTTTGATTTCATTGTTTTAGGTGCTGGCATTAGTGGCATAGTTTTATCACATGTTTTAGCACAGCATGGTAAAAGTGTTTTACTGTTAGAAAAACGCAACCAATTAGGTGGTAATTGTTATGACAAGTTGGATGAAACCACCGGGTTACTGTTCCACCAGTACGGTCCTCATATCTTTCATACTGATAATCAGAAAGTAATGGATTTTATCCAACCCTTTTTTGAACTAAACAATTACCAGCACCGTGTTGGCTTACAACTGGACAACAATTTGGATTTAACGCTTCCATTTGACTTTAGCCAAATGCGCAAGTTGTTGGATACAAAAACAGCTAGCAGTTTAATTAACTTCTTTCAACAGCACTTTCCAGCTGAAAAGCACTTAACACTGATGCAGTTACAAACAATTAACTTTGCTCCAGTCCAACAGCTGTACCAGTTTTTAAAAACAAAGGTGTACGGTCCTTACAGTGTGAAAATGTGGGGCATGCCATTAGAACAAATTGATCCTAGTGTATTGGGTCGAGTCAAAATTAGTTTAAGTGAAAACAGTAGCTATTTCCCTACAGCCACTATTCAAGGTCTACCTAAGGGTGGTTATACAAAGGCCTTTACAAAGATGGTGGATCATCCTCTGATTGATTTAAGGTTAAACTGTCCAGCTAACTTAATTAGCGTAAACAATAATCAACTCTTGTTTGCAAATCAACCAATTACCAAACCAGTGGTTTATTGTGGTTTAATTGATCAGCTGTTTGGCTATTGTTTTGGGCGATTGCAGTACCGCTCGCTCCACTTTGAATGAAAGCGTTATGCTGTAAAACAACACCAAGCTTACCCCGTAATGAACTGGCCACTCCACCCAACAATAACGCGCCAAGTGGAATACAAACAGTTAACGCAAGAGGGTTTGGAGTCTAACCAAACGATTGTTTCGTGTGAAACTCCTGGTGCTTTTAGGGAAGGCGATCCCCGCTTTATGGAGCCGTACTACCCGCTAAATGATGTAAGTAATAATGCCTTGTTTGCGCGTTATTTAAAGTTAGCTAACGCTATTCCTAACATACATTTGTTAGGTCGGTTGGCACTGTACCAGTACATTGATATGGATAGAGCAATTGCTCAAAGCTTAGCTAAAGCCGAACAACTATTACAGTAATGGAACAACAAAAAATTAGGAACTTTTCAATTATTGCCCACATTGACCATGGTAAGTCAACGCTGTCAGATCGCTTAATTGAACGCAGCATTGGCTTTGAAAAACGCTTACTGCAAGCGCAAATGTTGGACACCATGGCGATTGAGCGTGAACGGGGTATTACTATTAAACTCAACGCCGTGCAGCTCAAAATGGCCCAAGGCAACCAGCAGTACTTGTTTCACCTAGTTGATACTCCGGGACACGTTGACTTTACTTACGAGGTATCACGCTCTTTAGCAGCTTGTGAGGGTGTTTTACTGTTAGTTGATGCTACCCAAGGGATTCAAGCACAAACGATTTCCAACACTTACTTAGCATTGGAAAACAACCTCGAAATTATTCCGGTAATTAACAAGGTGGACATGGAGAGTGCTGATGTCGAGAAAACCAAACAAGCCTTTCACCAACTGCTAGGGGTGGATCCCAACACCATCCCCTTAGTGTCCGCTAAAACCGGGCTTGGTATTGACCGGTTAATCACTACCATTATTGAAAAAGTACCCCCTCCAAAGGGCGATGAAAGTAAACCACTCAAAGCCTTACTGTTTGACAGCTATTACGATCCTTATAAGGGGGTAGTGTGCTTTATTCGAATCTTTGAGGGGAGTTTAAAACTCAATGATAAGATCCGCTTTGCTAGAAGTAACTCGGTTTACCAAATTGTGGAATTGGGAATTAAAAACCCCTTTTTTGAAAAACAAGACGTGTTAAAAGCCGGGGAGATTGGTTGGTTTTCCGCTGGGATTAAAAAACTAAGGGATGTAACGGTTGGTGACACGATTGTTCACGCTGAGGACACGACCACTCCACCCTTACCGGGTTACAAAAAGGTGTTACCGATGATTTACTGTGGTCTGTACCCGATTGACAACAATGATTACCAAAACCTCAAAATGGCCATGGAAAAGATTATTTTGAGCGATGCGGCCTTGGAGTATGAATATGAAACATCACAAGCATTGGGCTTTGGGGTGCGTTGTGGTTTTTTGGGGTTGTTGCACATGGATGTAATTAAGGAGCGGTTGGAGCGCGAGTACAACCTTAAGTTAATTTCCGCTCCTCCTTCAGTGCGGTATAAGGTGTTGTTAACCAACGGGGAGGAATTGGAACTCGATAACCCTTCACTCTTACCAGAGCGCTCGCGAATTAAATCAATTAGCGAACCGTTTGTACGCGTTTACATTGATCTACCCGATCATTATTTAGGTACAGTAATTGATCTGTGTCAAAACTTCCGTGGGCAATACGAAAAGTTAGAGGAAATTGACATTGACCGCAAACGCTTGGTCTACTTAATGCCCTTGGGTGAAATTATCTACAGTTTCTTTGATAAGTTGAAATCAATCACTAAGGGTTATGCTTCGCTCAATTACGAGTTTGACCAGTACCAGGTGTCCCAATTGGCTAAGGTGGAAATTATGTTAAACAAACAGAAGGTGGACGCTTTATCTTTTATTGCCCACCATGACTTTGCCTTTCAACGGGCCAAGAAGTTCTGTGTCAAATTAAAGGAACTAATTCCCAAGCACCTGTTTGAAATTCCAATTCAAGCCACAATTGGAAGTAAGGCAATTGCTAGAGAAACGATTAAAGCAGTGCGTAAAGATGTAACAGCAAAGCTGTATGGCGGGGATGTCACGCGGAAAAAGAAGCTGTTGGAAAAACAAAAGGAAGGGAAGAAAAGACTGAAAGCAATTGGTAGTGTGGAGCTCCCCCAAGAGCTGTTTAGTCATTTGCTCAAAGATGAAGATTAACTTATTTATAATTGATTACTGTTTAATTACTGATACCTTGTAAAGGTATTAACTAATTATTTTGATCTTTTATAAGTAATGATTAAGGATTTTGATCCTAGTGAGTTTGTCGGTAAAACTCCTACCAAGATTTTTGCCTTTGGTGGTATCCAAGAAGTTGGAAAAAACATGTATGGAATCGAGTACGATGATGAGATTATCATCATCGACTGCGGTATTAAGTTTGCTAGTGATGATCTACTAGGGATTGATGGTATTATCCCGAGCTTCGAATACCTCATTGAAAACCAAGCTAAGGTTAAAGCACTGTTTATCACCCACGGCCATGAAGACCACATTGGTGGGGTGCCTTACTTGTTAAAACAAGTCGATGTACCGGTGATTTACGCACCCCGGATTGCCGCTTCTTTGATCTTAAAGAAGGTCAACGAACACAAGGATGCTAAGCTCAATAAAGTGGTCGTATATGATGACTTTAGCAACTTTGAAACCAAGCACTTCAAGATTGACTTTTACCGCGTCAACCACTCGATTCCTGACGCCTTTGGTGTCTGTGTTCAAACTCCCAACGGTAACATTGTTGAGAGTGGTGACTTTCGCTTTGACTTTGCCGCTGGCGGGGAAATGCTCGATGTGCATAAGGTCGTGAAGATTGCCGAGCGCAATGTCCATGTCTTTATGTGTGAGACTACTAACGCCGAAATCCCGGGGTTCTCCCAGAGCGAAAAGTTAATTTACCGCAACATCAACAAGATTATTAAAGAAGCACGTGGTCGGGTCATTCTCACTACCTTTGCTTCCAACATTACCCGGATTAACGAAATTATTGAGATTGCTGTTAACAATAAACGGAAGGTCTGCTTGTTGGGTAAGTCGATGGATGTGAACGTCAACATCTCGCGTAAGATTGGCTTAATGGACATTGACAGCAATGACATTGTGGAAGTCCGTGACATTAAGAATTACCCCGACCGCAGTATCTTAATCTTGTGTACGGGTTCCCAAGGTGAAGACTCAGCAGCCTTAAACACAATGGCACGGGGTAAACATAACTGGGTTAGCTTAAAGTCCACTGACACCATTATTATGTCCTCCAACCCGATTCCGGGGAACTATGCAGCGGTGGAAAATCTCTTGAATGAGCTTTCGAAGTACGGGGTGACCATCTTTGAAAACTCACCGAACATGAAGCTACACGCCTCTGGTCACGCTACCCAGCAAGAGTTGCAGTTAATGTTAAACCTCGTGTTTCCAAGGTACTTAATTCCGATTCACGGGGAGTACAAGATGATGCGCACCATCAAAAACATAGCGCAAGAATGTGGCATTAACGGTGATGATGTTGGTTTGCTAGCTAACGGTCAGGTGATGTACCTAATTGATGGCAAGCTGTATTACTCAGGGGAAGTGATTAATGCTGACCCGATTTACATTGAAAGCCGCAATTCTTCTCCAGACTTAGCGCGGGTAATTAAACAACGCCAAATCTTGAGTCGGGAGGGAATGTTTGCTGTGATTGTAGTCTTTGACAAAAACAATAACATTTTAGGGATGCCTACTCTGATTACTCGGGGTTGTTTCTTTGCTCTAGATTCCAGTCCGTTAATGACTAAGATTACCCACTCAATTAAACGCGGTTTGGAAAACGTTATTCAAAACAAGCGTTTTAATACCCGTGAACAGATGATCAAGGAATTGAAACGAGTATGTAAGGAAACGGTGTCTTACTTCATTTGGAAGAACAAGAGTCGTAACCCGTTGATTTCAACCGTATTGTCTTGAGTTTAAATCTTAGTTTTAACTAATTCTCAATTGGACCCAATTATTGGAAATTAACACCTTTATAAGGCTGTTTTTGCCAGTAGTTGGGTTTTCTTTTGTTTGCTCTTGAGATCTAATTAATGCATACAAAAAGCCATTAATTGCCCAAAAATCAGATTCTTTTTACTTTCTTAAAAATATTTTTTTAAGATTTGCCATCTTTTTTCGCTTGGGAAGATGAAATTTAAGTATGGAACGGTTGTTTTGGGCAGTTTTTTAGGGCTTTCTGTGGTGTTAGCTGCCTGTGGTGCTCGTGGTAAATTTGACCAATTTGATGATGGCAAAATTAAGCTAGCATCATCCTTAACTTCCAAAGCAGCTGCCAATGCTTTACAAACAATAATAGAGAAGTACAACATTGTCAAAAGCGGCAAGGATTACCCCATTGAAATTACCCAAATTGCTGGTGGTTATGACGGCGGGCGGACTGATCTCCAAACCCGAGTTAGTGTTAAGGATAAAACGAACTTTTATAACCTTATTTTGAACTATCCAGATTTAGTTTCTGTGTTAGCGCGCAGTGGAATGGAACTGCTTTTTGACAAAGTTAATGTTGATAAATTAGAGCCGAACTTCTTAAAGTTTAATGAACAGATTAGTGGAGTCGCTAAAAGTGGTAACTATGGTATTCCCGTGTCCCTTTCAACTGACATTTTGGTCTTAAATGGTCCGGTGTTGCACTATATTTTGAACAGTGCGAAGAAAGAAGAGGCTAATTCTCAAGTTAAAAGTCAAATAAAAACATCACAAATTCAAGCTAAAGGTTCATTAACAATTGATACAGATGAAAATACCAAATCACTTTGAGAGAAAATTCAAAATTCAGCCAAAGCTAATGGTGAAACTAATCAAAAAGGACGAAAAGCTGCAAAATCAAATAAAACTGCATTAGTTCAACTTAAAAATGGCGCTGATACTACCACTAACGAAGAGAATAAAGATACCAAAACTAGTGATGATAAAGTTAAACAAACTTGAGGTGATTATGTAGAAAAGGATGATGGTCTTAAAAACTATACCTTTAGGGCTAGCGTGTTTGAAAACTGGCATGATTTCCTCGATTTTTCGACAAGGGTAGCTAAATCTTTTACTGAAAAGGTAAGCAACATTACCAATAAAAAGGGCACTGATATTCAGGGAGTGCTTGGTGTCGACAGTTCTCCTAATGTTTTATTTGCCTCTGTATTTGCTGCAGGTGGTGGTAATTATGAAAACTTCTTTTATAAGGTGAAAGATGGTCGGGCTGATTTTAGTAACTTTAAGAACAAGGGTAGTTCTTACCAAAACCTCCAAAGCGTTTTTAAAGACTAAAGTGAACTAATTAAAAGTAACGGTCTTTTTGTCAATAAAGGTGGTTCTTATTCTTCCAATTTCCAGAAGTTTCACCAATTAGCTTACAGTATTTCTTCCACTTCTGGCTTTAAGTATTCGTTTGCCGATAAGAACGCTAAGCGCTTGAAGTTTAGTGACGACAACACCTTCGTTGAATATCCTGATTTTACTCAGGAAATTAAGTCGCCAGACCAACAAAATGGTCAAAATACTGATGACAATAACAGTAAAAAAGAAGAAAACCTTTTAGGTACCTTTGAAGTTAAAGATTACTCGAAATCTAATAAAGGCGGGAAAGGAAAAAGTAAGGGTAAAGGCGGAAGCAGCAGTAATATGAAGACTATTTACATTTACAAAGGCTCTATTCCTGAAGATAAAAAGAAAGGTGATAATGCCATTTTAGTTACTGAGAAAAAAATTATTGATGCTTTAGAAAAAGCTGCTAATTCTAATGGTGAAAGTACACAATCTAATCCAAAAACCATAGCACTTCAAGCAGCTAAAAAAGAAACTAAAAAGAGCAATAAGAAAGAATCAAAAACGATTGGATATACAACTACTGGTTATGTCAATGCTGATGGTAAACATATTTTTGATACAGACAGAATTAACAATGAGAGGTTTGATCGTAAAATTATTATTGGTGCTACTCTAGAAACTTTAGACCAATCTAGTACACTCCAAAGTGGTGAAGCAATTGTCTTACCAGCACCTGGTAAATATAAGAGTAGTGATCAAAAAACGGTTATGACAGCGCAAGGCCCCAAGATAATCGGGATCCATGCTAATGCTAAAGAGAACGAAGAAACACAAAAATTTGTTAATTGGTTTTAAATACGCCAGAAACTTGAGAAAGTAAGAGGAATAAGAAAAACTCTAATGAAAAACAAACTGCAGCACAATACTTTGCTCAAGAGGCATCTTACATCTTGCCCTTAAAAGAAAACTTTGAAAAGGTTCAAATTAACGATAAAGGTGAGAAAAATGAAAATGACACAAAGGGCAATACTTATACCGCTAAAGCTTTAGAACTCTTTAAAAAAGTTTCTCAAAATGAAATCGTGAGTTACAGTGACCCAAGTGACTTTAGAAGTGGTAAGTTTCGTGATGCGATTGGCTCTAACTTTAACGCCATTATTAATTCCAAAGGAGACTTCAATAAATTTATTAGTAGTTTTACCGCAAGTTTAGGCTCAGACTTTTAGTCTTTTAAATACCCAATTGATTTATTTTTTAATCGATTAATTTATTTAATGGAAAAACAAGTAGATAAATTAGTGAAAACTTTAATTCATCAAAAAGCGTTTCAATAAATCCGGTTCTTAGAAATAAGGTTTTAATAAAAAAACATACCAGCAGTTAATGCTTCCAAATTAGCTGTGGGTATGTTTATTCTTGTCAAATAAATAAAAAAGCCATCTATCTAAAAGACAGACAGCTAAAAAATACTTTTTTTAACGATATTGAAATTAACAGAAAGATTTGTTAGCAAATGTGAAGGAAGAGGCTCTATGCAAACTTTCAATATGTGGCGTGTTGAAAAACCTTTAAGAATATGCACCAATTGGGGGCAGAAAAGCAGGTCTTCAACATAAATTTTCGTTTTCCTGCTCCCATTAGATAAATTGAGTTGCTTTTTAAAAGACGCCAAAAAGATTAAATGTTCTTCTAATTTGGTTTTTTAACTTCTTCTAACTATTAATAGGGTATTTTACTTTCTAAAAATTTTTTGAAGTTATTTTGTCACATTTACTTTTTGGGCAATTTAGATCATGAATACCGAAAAAGTATAAGATTCATTAAGTTAACGAGGTATCACCTCTTTTTAAAACAAGCCCAATCGCAACTCGTTTCTTTGTTTAACCAAAACTTTTTGCAAGTTTTGTTGGCATTTCTAAATAAATCTTTCAATTTATTTAGTGATGACACAAAAATTAATATGTTGAACTTTTTAAATAAATCAACAATGGTATTGATCCCCGTTGGCAGCAGTGCTCCAAGTTCTAGTTCCTTTTTAAATCACCTCAAGAAAGAAGGTATTGCTTAATGAAAGAGAAAATTTCTAAAAAGGAATACAATGCCTTAATCCGTAAAACTGGCGAAAAGCACTTTGACGGCGAAAAGGAAGAGTACGGTGATGGCACCGTTGGACTTTGAACTTATGAATTGCGAAAATATAAACTTAAACCACCTGTGAAAGTTAAATACGTAACGCAAGAAGAGTTTGGCGAATTCAAAGATGCCACCAACCAGCGGCTCACAAAGATTGAGAACGCTTTGGTCGCTCAGGGCGAACAAATTAGAGCTCAGGGTGAGCAATTAAGTCAACTAATTAAAGTTGTGCTTCTTCAGGGCAAACAAATAAAATCTCAAGGTGAGCAAATAAAATCTTAGGGCGAACAAATCAACAAATTAACTCAAACTGTTGAAAAGCAGGGCGAGAAAATTGAAGTCCAAGGACAACAGATTCAAACAGTTAATGAAACTTTGAAGTTTGTAGTTGAAAGCCTTGAAACTATCCATAAACGGCTCGATGTAATGGAAATTCGTTTAGACAAACTCGAGTCCAAATATATCTGATTCAAATAAGAGTACCAGCAGTTAGTTCTAAAAGCAAGCTGTTGGTATTTTTATTGATCTAAAACTACCTAAAAAAATTTTTTGCAATTTTGTTGTCACATATAAAGTCTTAGTTCAATTTATTTAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGTAAAGAGTCTGATCCCTGTTGACAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAGGAGGATTTAAACATGAAAAAAGTTAAAGTTCTAGAGAAGCCATCTGTTGAAAGAATGATAGAAGTTTTAAGCGACTCTGAAATATTGAAGTTTGATTTTTCTTGCTATTATTTACCTGATGGTACTAATGAGACGCTCATAAGAATCTTTTTTCAAGAAAAAGTTTCAAGTTCTGTAGTAACAAAAGATAAAAATTCTTTAAGACTTGATGAATACTTAAAAGACTTTATTAGCTTCTTAAAAACACAAAGAAAAAACTAATAGCGTAAAAATCCAATAGTGAAAATTCACTGTTGGTATTTTTATTTATGTCAAATAGAAGATATTAGATGGAAAATTCAAATAGAAACTATAACACACAGAGCTAAGTAATTTTTATTTGTACTTTTTCTTGTTTAAAAAGCAGTTACTATTTATTCTTTCTTTATTTCATTTTTATCTTTGAAAAATGAGAAAGTTGACAATATTAAAAACTTAAACTTTTTTTAAGATTCTGTAGATTTTCTGGGTTTTAGTGCATGAAATTGAAATATGGAACCATTATTTTCAGTGGTCTTTTGGGAGTTTCCGCCATTTTAGCTGCCTGTGGTGCACGCGGCAAATTTGACCAAATTGATGATGGCAAAATTGTTTTAGCTTCTTCTCTTACATCTAAGGGTGCAGCTAATGCCTTGCAAACAATTGTTAAAAAGTACAACGAAGTTAAAAATATTGATGACTATCCCATTGAAATTATCCAAATTGCCGGTGGTTATGATGGCGGTCGTGGTAACTTACAAACAAAACTAAGTGTTAAAGACAAAAATAGTTTCTATAACTTAATTTTGAACTACCCAGACGTAGTTTCTGTTTTAGGTCGAGTAGGCATGGAATTGCCCTTCGATAAGGTTAGAACAGATAAATTATCACCCCGTTTTCTTGATTTCAACAAGCGAATTAGTGCCATATCTAAGCAAGGTATTTACGGCATTCCAGTCTCTTTGTCTACTGAAGTTTTAGTTTTAAATGGTCCGGTATTGCACTATATTTTGAGCAGTGCAAAGGGTAGTAGTGACAAAACGCAAGTAAGTCAAACATCATCAGGATTAAATCAACAAAAAACTCTTCAAAAACCCTTAAAGATTGATACAAGCGATTCAAGCACTTCTTCTCTTTGGACTCAAATTGAGAACGCCGCTAAAAATAACGGTAAAAAAGCAAATAATAGCAAAAGCAATAGAAGATCCACAGATCAAAGTACACAAACACATAACGATCAAGGCGATGCTTCTGAAAGTGATAAAAAGATTAAAGAATCTTGAGGCGATTATGAAGAAGTGGATGGTGGCTTAAAAGGCTTTACTTTTAAAGCTAGCATCTTTGATAATTGGCACGATTTACTCGATTTTTCTACTAGAGCGGCTAAATCTTTTAAAAAGATAAAAGATAACAATACTAAAAAAGGAACTGATATCCAAGGCATTCTTGGTGTAGATAGTTCAGCCAATTCCTTATTTACTTCTGTTTTTGCAGCTGGTAATGGTGACTATGACAACTTCTTTTACAAGGTTGCAAACGGTCGGGCTGACTTTAGTAACTTTAAGAATAGAGGTAGTTCCTTCCAAAACTTACAAAGTGTTTTTAATGATTACAAGGGTTTAATTGATCAAAACGGTCTCTTTGTTAATAAGGGCGGTTCTTACTCTTCTAACTTTCAAAAGTTTCACCAGTTAGCTTACAGTATCTCCTCTACCTCTGGTTTCTATTATTCATTTGCTGGTAATAGTGCTAAGCGCTTAAAGTTTGGTGATAATTCTTTCATTGAGTATCCACAATATACTGTTCCAATTAAAGCTCCTTCAAAGAATGGTGATGGAAATAGCACAAACAGCAATAGCGATCTTTTAGGCACTTTCACTTTAAGTAGTGGTAAAAAGTCAACAGATAAAAGTAAAAGTGATAGTCAACAAAATCAAGGCAAGAAAGTAGAAGGAACACCTAACCAGGGTAAAAAGGCAGAAGGTGCCCAGAACCAAGGCAAAAAAGAAAATAACAGTACAACTATTGAAATTTACAAGAACAAAATTCCTGATGGTAAAAATGCTGGGAAAGATGCCATCTTAATAAAAGATAACAATCTCATTAAACAGCTAGAAGATGCTGCTAAGAAAAACGGTGCAGAAAGTAATCAAAAACAAGGTGGAGAGAGTAATGGTCAGAAAGAACAAATTATTGGTTATACCACCACTGGTAACGTTCGTGAAGATGGTAATCACATTTTTAGAGTAGACAAAATTAATGACGAACAATACGATCGCAAAATTATTGTTGGCGTTACTGTGGAAACTTTAGAACAGTCTAGCACTTTGCAAAGCGAAGAAGCAATTGTTTTAGCAGCACCTGGTAAATATAAAAGCACTGATAAAAAGAAGGTAACAATTACCCAAGGTCCGAATATTATTGGTATCCATGCTAACGAAAAAGAAAATGCAGAAACCCAAAAGTTTGTTGACTGGTTTTTAAATACAGAAGTAGATTGACCCGCTAAGGAAAATTCTTCTAATAAACAAGATCAACAAAATTCAACCAAAAAACAAACCGCTGCTGAATTCTTTGTTGAATCCGCTTCTTATATTCTGCCTTTAAAGGAAATCTTCGAAAATAAAGAGAAAAAAGAAAATACAAGCAATAGTGATAAAAATAAGAGTAGTAGTCAAAGGAAAAATACTTACGCTGAGAAAGCGCTAGAACTTTTTCAACAAATAAGTAAAGATGAAATAGTTAGTTACAGCGATCCGAGTGATTTTAGAAGTGGTAAATTCCGCGATGGGATTGGTTCTAACTTTAATGCTGCTGTAAGCTCTAAAGCAGACTTTAATAAGTTTGTTAAAGGCTTCATCGCCACTTTGGGTAGTGAAATTTAAGCGAATTTGTGAGCTAACTAATCACAATAATTTTAGGTTAAAACAAAACCTTAAAAAGGTTTAGTGAAAGAAATTAAACTGTTACTATCGTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAATTGGACATTAAAAAAGATCCAGGAGTTTTTCCTGTTTATTCGGCAGCTACTAATAACGATGGGGAGTTTGGTCGAATAAATTCTTATGACTTTGATGGTGAATATGTTACTTGAACAGCTGACGGTTATGGAGGGGCTGTTTTTTATCGTAATGGTAAATTTAGCATCACAAATCTTTGTGGTCTTTTGAAAGTAAAAAATAAAGAAATTAGTTCAAAATATCTTGCTCATATTTTGAAACTAGAAGCACCGAAATTCACAAACCGTGTTTTTAAAAATAGACCTAAGTTAACACATAAAACAATGGCCGAAATTCCCATTGATTTCCCACCCTTGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGTGCCGAGCTAAGAGAGCGCAGGAAGCAGTATGACTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAATAAGGGATATTTGTGAAATAAATCGTGGTCGAGAAATAAATGAAAAATACCTTCGAGAAAATCCAGGTGAATTTCCAGTTTATTCCTCAGCAACAACAAATGGTGGTTTAATTGGAAAAATTAATGATTACGATTTTCATGGTGAATATGTTACTTGAACAACGGGCGGCGCACATGCCGGAAATGTCTTTTACCGTAATGAAAAGTTTAGTTGTTCACAAAATTGCGGATTGTTAGAAGTAAAGAACAAAAATAAATTTAGTTCCAAATTCTTATGTTTTGCACTAAAACTACAATCTAAAAAATTTGTGAATTATGCCTCTGCAATTCCGGTTCTAACAATAAAGAGAATTGCAGAAATCGAACTCTCCTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTTTGTTTGCATTTGAAAAACTTTGCAACGATTTAACCGAGGGGATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTTCAAAATAAGAAATTAGAAAGTCTCAAATCTCTTTAATAATAAAAGTTAGTTCAGCAAACCACAAACTGATCCCACTTCTCCCCCACGCTCACGCGCTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTTCTCGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGCACAGGTATGGGAATGACCACCGGGGGAGCAACAGTAGTACGAGTGGGGTAACAACCCAGGGACAACAATCGCAAAATGCATCCGGAACCGAACCCGCACCCACTTTTTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTGGGTGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGACCCACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGGCGGAATGATAAGGTTTCAAGTGGACAAAGTGACGAAAACCACACCAAGTTCACGAGCGCTACGGGCAGTGGGCAGCAGGGCAGTTCTTCAGGTACAACTAATTCCGCGGGCAATCCCGACTCGTTAAAGCAGGATAAGGTTGATAAAAGTGGTGATTCGGTAACGGTTGCAGAAGCCACTTCAGGGGACAACTTGACGAATTACACCAACCTCCCTCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTCTTCCTCCGCACCCTGTTGGGCAGCATCCCGGTGTTGGTGAATAAGAGTGGCCAAGATGATTCCAACAAGTTCCAAGCCACCGACCAAAAATGGTCTTACACCGAATTAAAGTCGGATCAAACCAAGCTCAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAGGTGTACGGTCTGAGTTCCACTCAAGATTCTAGCACCGGAGCCGGTGGCGCTGGAGGTAACACCGGAGGCGACACCAATACCCAGACTTATGCACGACCCGGAATCGGCTTTAAATTACCCTCCACGGACTCGGAATCTTCCAAAGCCACCCTGATCACCCCCGGGTTGGCTTGAACAGCGCAAGATGTCGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGCTCGGCGGGTGGTTGGTTACCTTCACGGATTTTATCAAACCCCGGTCGGGTTACCTCGGGCTCCAGTTAACGGGCTTGGATGCAAATGACAGCGACCAAAGGGAGTTAATTTGAGCCCCCCCGGCCCTGAACCGCCTTTCGTGGCAGTTGGGTCAACCGCTTGGGCTGCGTCGAGAGTGTGTGGGATTTCAAGGGGGTGTGGCAGGATCAAGCTCGGTCCGACTCGCAAGCAGCTACAAGTACCACCACCGCAACGAAGGCTACCTTATCGGAGCACACCAATGCTTTGGCCTTTCAGGTGAGTTATACCGACCAGGATTCGTACAAGGCTTCCACTCAAAGCTCCGGCCAAAACCAAAACACCTCCCCCTACCTGCACTTGGTGCAGGGAAAAAAGTAGGTTCCTCTGATAAGCTTTCAGACGATCTTAAAAAACTGTTGGACCCCAACGAGGTTCGCGCCAAGCTGCGCCAAAGCTTTGGTACAGAAAATTCTACCCAACCCCAACCTAAAGCCCTCAAAACAACCACACCGGTATTTGGGACGAATAGTGGTAACCTCGGCAGTGTGCTTGGTGGTGGGGGTACCACGCAGGGCTCAGGCTCAGGTCAATCTGGTGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAACTATTAATAGGAACTTTTCTTATTAATAGGAACTTTTACTTTCAAAAAATTTTTGAAGTTATTTTGGCACTTTCAAACTTTAAAGACCATTTAAAAGGCATAAAAGGATAGTTATGTTTTTAAGAAATATTTTGCGATTCAGTTCGCTTTTGGTTTTGTTTGGTTTTGAGTCTTTTCTTTTGAATGTTCAAAAATTCTTTGAATTAATTTTTGGCAACTTTTCCAATTTATGCAAATTTACATGATGTAAAACACTCACGATGATAGTTTTTAAAAAATATTTATTTTATTTAGATAGCCAAATAATCCAGTGATCTGTTGCTGCCCACCTCTTTAAACACTTTTACGTTTTTCCAATTAATAATAAAGGAGGTCTTCACCATTAAACGCACAATTTCACTTACTTTGTTTAAGAAAAGACTCAACAAAGATAAGATTAATAATTGTCATGTTTGGGAAGAAGAGTTACCTGATGGTAGCTATGACATGGGATTTAGTGACAATTTAAACCATATGGAAAAACGAAAAAGTGGTTATGTTACCCAAAAGCAGTTTAGCGAGTTCAAAGATGCCAATAATCAAAGGCTGATAAAGATTGAAACTACTTTAGCTACTCAAGGTGAACAATTAAATCAATTAATTAAAGTTGTTATTCTTCAGGGCGAACAAATTAAAGAACTTCAAGTGGAGCAAAAAGCTCAAGGAGAACAAATAAAAGCTAAAGGAGAACAAATAAAAGCTCAAAGCGAGCAAATAAAAACTCAAGGAGAAACGCTTAAATTGATCCTACAAGCACTTGGAGGAATAAATAAGCGCTTAGACAAAGTTGATCCGCCTAAATAGCTTTAGGCTAAAAAATAAAAGTGCCAGCAATTAATCTATAAAGGTTAGTTGCTGGTATTTTTATTCATTTGAAATAAATAAAAAAGCCATCTGCTCAAGGCGAAAAGCAGACAGCTCAAAAATTTTTTAATGATTTTGAAAACTAACGAAAAGTGTTAGTTTGAGTTGTAAGACAGATATGAATGTTTATTAATTCAAAAAAATGTTCGTTGCTTAATGAAAGATGTGCTGAAAACCGTAACGGTAAGATAGATTTGTAAAAAGAGTAAAGAACAAAACCATGTACGCAATAACTAAATTGATGGGAACAGAAAATCAGGCTTTCAACACAGAATTTTCGTTTTCCTGCTCCCAATAAATAAATTGATTTAGTTTTTTAAATGTGACGTCAAATTTCAATAAAAAATCAAAATTTCAAACCAAAAACTATAACACACCAAGCTCATTGATTTTAATCTCCAGTTTTTTCTCATTTAAAAAGGATCAAAATTTGTTTTTAATAGTTTCGATTTTATGTCTTCTTTAATCACTGAATTTTTAGGAATTAAACTTGTTTAATCTTTATTAAGATTTCTCCAAATTTTGGGTTTTAGTGCATGAAATTTAAGTATTGTGCCATCTTTTTCAGTGGCTTTCTAGGTCTTTCTGCCATCTTAGCTGCTTGTGGAGCGCGGGGCAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTTCTCTTTAACTTCAAAAAGTGCATCAAACGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCATATTGAAATTACCCAAATCGCTGGTGGTTATGATGGTGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAGGACACCACTAACTTTTACAACCTTATCTTGAATTATCCTGACTTAGTTTCTACTTTAGGTCGTGTTGGGATGGAACTACTGTTTGATAAAGTTAATACAGATAAATTATCCGACCGTTTCTTAGATTTCAATAAACGAATTAGTGCAATTTCTAAACCAGGGATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAAAAGTGCAAAAGGTGATAGTGACAATGTTAAAGTAAGTCAAAGGACTGGAGAAACTACTCAAAAAAGTAAAGTAACTAATCCTTTGAAAATTAATACTCAAAACGATCCTGCTACAAAAGATCTTTGAGAAAAAATAGAAGCTTCTGCAAAAGCTAATGGTAAAAGCAATAAGGATGGACAAACTAAGGGTAAAAAGAAAGTAGAAAAAAGTTCATCTTCTTCGTTAGTTAATTTAAAGCAGTCTACGGACCAAACAACGACAGATGACGGTTCTCAAAAAAGTGATAACAAAATCAAAGAATCTTGAGGCGAATACCAAGAAGTAGATGGTGGTCTTAAAAATTTTGAATTTAAAGCGAGCATCTTCGAAAATTGACATGACTTACTTGATTTTTCTACTAGAGTGGCTAAGTCGTTTACAAATGTAAAGGGAAAAGATATCAAAAAGGGAACTGATATCCAAGGTGTACTTGGTGTTGACAGTACGCCCAACTCATTATTCACCTCTGTTTTTGCAGCTGGTGGCGGTGATTACAATAATTTCTTCTACAAAATTGAAAACGGCCGTGCTGACTTTAGTAACTTTAAGAATAAAGGCACTTCCTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTTCAAAAGTTCCATCAATTAGCTTACAGTATTTCCTCTACTTCCGGTTTTTTCTATTCGTTTGCCGGTAACAGCGCTAAACGCTTAAAGTTTGGTGATAACTTTATTGAATTTCCTCGTTTTACTCAGGAAATTAAAGCACCTTCTACTGAAAATGGCGGCCAAAGCAATCTTTTAGGAACCTTTGAAGTTAAAGATAGCAGTAAATCTAAGGAAGTGAAAAAAACTAATAGGAAAGAAGATGGTGCTCAAAATCAAGGTAAAAAAGAAAGCGATAAGAAAACCATTTACCTTTACAAAAGTCAAATTCCATCAGACAAAACAGAAGGCGAAAATGCCATCTTAATCAAAGAACAAAACGTTATTAATCAGCTAGAACAAGCAGCTAAAAAAGATGAAAAAGGAGAGACTGTTACAAACAAAGTTGCATCATTAGAAACCAAAGCTGCTAATGCCAAAAAGGATAGTAGTAAAACCATTATTGGTTACACCACAACTGATAATGTTCATGAAGACGGTAAAAACATTTTCAAAATTAACAAACTCAAAACTGAAGACTATGATCGTAAGATTATTGTCGGTGCCACTGAAGAAGTGTTAGAGCAATCTAGCACATTACAAAGTGATGAAGCAATTGTGTTACCAGCTCCTGGTAAGTATCAAAGTGGTGACGCTAAGAAAGTAACCATCACCCAAGGTCCTAATATCATTGGCATTCACGCTAACGAAAAGGAAAACGCCGAAACGCAAAAGTTTGTTAACTGGTTTTTAAACAATACAGAAAGTTGAGAAGTTAAAAATGGAAAAGATTCCTCCACTAAACAACAAACTGCTGCCGAATTCTTTGCTGAGTCAGCTTCTTATATCTTGCCTTTAAAAGAAATCTTTGACAAAGATAACAAAAAAGCTACTGAAAATAAGGACAATAATACTAGCAATAAAAAACAAGCTAATACCTATGCTGAAAAGGCGTTAGATTTGTTCCAACAAATTAGTAAAGGTGATATAGTTAGTTACAGCGACCCTAGCGATTTTAGAAGTGGTAAATTCCGCGATGGGATTGGCTCTAATTTTAACGCTGCAGTAAGTTCCAAAGCTGATTTTGACAGGTTTGTTAAAGGCTTCATCGCCACTTTGGGTAGTGAAATTTAAACGAATTTGTGAGCTAACTAATCACAATAATTTCAGGTTAAAACAAAACCTTAAAAATGTTTAATGAAAGAAATTAAATTGTTACTGTTGTGCAAATCAAAACTTACAAAATCAAAGATATTTGCGATATTAAACGCGGTAGAGTTATCAGCAAACTATATATCAAAAACAATCCAGGCGAATTCCCTGTTTATTCATCGGCTACAGTTAATAATGGTGAAATTGGCAGAATAAAAGATTGTGATCTTAAGGGTGAGTATGTGACTTGAACAACTGATGGCGCTCAAGCTGGTAGTGTTTTTTATCGTAACGGTCAATTTAACGCAACCAATGTTTGTGGAATTTTAAAGGTAAATAATGATGAAATTTACCCTAAATTTTTAGCCTATGCGCTTAGATTGAAAGCACCTAAATTTGTAAATTATGCTTGCCCTATTCCTAAATTAATGCAAGGAACTTTAGCAGAAATTGAGCTAGATTTCCCAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGTCTTCTATAGCGATTATTTACTTAACCCAAAAAATTGAAAATAGGAAAATAAATATTACAAGCTGGGAGAAATAGCTCAAAAAGTTTTAGTAGGTGGTGAAAAACCTGCTGATTTTAGTAAGAAAAAGAACGAAGTTTATAAATATCCAATTCTTTCTAACAGCAGTAAAGCTGAGGAATTTTTGGGTTACAGTAAAACTTTTCGAGTTGAAGAAAAATCCATTACGGTAAGTGCTAGAGGCACAATTGGTGTCGTTTTCTATCGTGATTTTGCTTATTTGCCTGCTGTTAGTTTAATTTGCTTTGTCCCTAAAGAAGAATTTGATATAAGGTTCTTATTCCACGCATTAAGAGCCATTAAATTTAAAAAGCAAGGCTCTGCAACAGGCCAGTTAACAGTGGCACAATTTAAGGAATATGGTATTCATGTACCTTCGCTGAAAAAACAGAAAGAAATCGCCGCTATCTTAGATCCACTTTACAGCTTTTTTACTGATCTAAACGAAGGTCTTCCTGCTGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAACTTTTTATTTAACTGAATTCAAAAACAAAAAGAACTAGTTGAACAAGCATCCACTAATTAACGGGATCTTTAAGGTAGAGTAGTTCTAAATTTTGGGGATTTTCCACTAGTTCAAACCGTTCGATGTTGTTTAGGAATAACTCCTCAATACTGTCGATGTTTTCAAAGTTTTCATATAACGGTAACTCGGTATTTGCTTTGCACAGCTTGACAAAATCTGGTTTAGCTAAGAGTGCTATTTCACTCGTAGTTTCGGTAAACAAACCACAGTAGTTCTTTTCATTGCCAGCACTAATTTTGCTAATGCCACAGCCATAAGGAATTTGAAGGCGGAGTGAGTTTAAAGCAAAGAGTTGACAGTTAGGGTTTACTGTGCACCAAGTTTTAGCAATAATGGCACCAACGCGTTGACCAGTAAAGCTACCCGGTCCAGTGGTTACGTAAAACTGTCTAACAGTGTTATTGCGTACTTTGTTTTTCTTTAACAATTTTTCCAAGTGATACACAGCCAACTCCGTGAGGTTTTGTTGTACTGGTATTGTTAGTTGATCCACCACAGTATTCGTTTTAAAATCCAACAGTACGATATTGAGGTGTTTGTAAGCACAGTCCAAAAAGAGCTTGTACTTATTGAAAAAGCGCATAAAGGGAATTAGCAATTTTCAAGTTTAAATTATCTAACTTATCGCTAAAAGTTTTATCTAACTGTGCTTCAAAACCCATTTCCTTATTCAAAGTGGGGTGGATAAAACTAATTTGTTGCGCGTGTAAATACTGGCCATATTCAGTAGCTTGCTCACCCTTCAAACCGTATAAGGGATCGTTGTAAACGGGGTGTTTAATAAACTGCAAGTGTACCCTAATTTGGTGAGTTCGTCCGGTTAACAGTTCACACTTAATTAAAGCAGCCTTTTCGTTTTGGTTTAACACCGTAAACTTAGTAAAAGCATTCTTGGCCTTGTTAGAACTGTTCCCCACCTTTCACATGACCTTATTGTTACCCACTCGTTCTAACGGGGCGCTAATGGTACCAGACAAACTGTCTAGGGGAAAATGGACTAAAGCTAAGTAATAACGCTTCATAACCCGTTGCTGGAGCTGTTTTTGCAAGTGCAGCAAGCTACTTTGGTTTTTCGCTAGCATAATCACACCACTCGTATCACGGTCTAGGCGGTGGACTAAAAACACCGGTTGGTGGTTGCAGTGAAACAAAGCGGCACCCAGTAAACTTTCACTTTCATTAAAACTAGTCGGGTGGCTAATTAAACCATTGGGTTTATTGACCACCATAATTTGCTCATCTTCATATAAGATGTCCAAGGGAAAGTCATAGGGCTGGACTTCAACTGGCACTTTTTCAAACAGTTCCTCACTCCAATTAACGTGTACCCGGTCTTCTGGTTGCACTAATCAACCATTTTTAGTAATTTTTTTACCGTTAACACTCACCAAACCATCGACAATTAACTTGGCTACTTTAACCCGGGAAAGGTTGAGTAGTGTGGCCAAAAAGGTATCTAAGCGTTGGGCTGTAGTAACACTAAAAGTTTGTTCCATTAAGATTTAGTTTGCTTCATTTCTTTGAAGAACTGAATTAAAAAGGAGAGGAACAGACCAATAAAACTAAAGGTAATGCAACAGTCCGCTAAGTTAAACACGGAATTACCTCCACTAAAGACAAAGTAATCTAGTACTTCACCACTACCCGAGGTTAACCGATCAAAGAAATTACCAAGTGAACCAAACGCTAAGGTAGTAATTCAAAAGATGTAGTTGTAACTAGTAGAGAAAACCAAAAAGAACAGTGCTATGAAAGATAGAAAACCCTGGAGAAAGTAAACAAGTCCTGGTTGGTCTGCCAATAAGCTAAAACCAACCCCCTTGTTACCGATAACATAGATACTAATAAAGGAACTGCGAGCTACATCTGTACCATTAGCACCATTAATACCCTTCTCACCATTTAAGGCAGCACGCAGCATAAATACCTGAAACAAAATGACAAAACTGACAAACAGGATTAAGGCAAGCTTATAGTACAAAAAGGGTTTGCGGTTAGCAAAAAGGATTTGCTTTAGCAATTTTGAAAAAAAAGTTGGGGCTTTTGCCATCTTGATGAGCTTAATTTTAAAAATGTAAAATAACGGCCATGGATTTGCTTACAACCGCAAACAAAACGCCGCGAATCGCCAAATCACGTCCTGTTCGAATTGCCATCATTATAACGCAGAAGACTAATGATCTGCACGCTACTGTACCCTGCTTTTTATGGCGCAAAGCAAGGTATGCTGTTGACCTGATTTCCGCTGAAGCCAAAGCTAGCATTATGTTAGAGATGGGGATTCATGTGCGCTGTGACAACACTTTGTCCAAAACTAACTTTAACCAGTATACCGCTGCTTTTATTCCCCATGGCAACACTACTAGGTTGGTGGAAATAGACAAACTAAGAAAGGACTTGGAGAAGTTTGTCTATAAACCCAAGGGACCAATGCGCTGGTTGTTTAGCAGTGGTAATGGTGCTTGTGTTTTGAAGGAGTTTGACTTGATTGCCCCTGACCAATTAGTCACCGTTCAAAACGAAAAGGAAATGGTTAAACTTTTGGGAAAAAATTTCATCAAGCAACCAGTACATGTGGACAAAAACATTATTAGTTGTGCCAATTCTTGTGGCTTGACTAAATTCTCCTTTAAGGTAATTGAAGAGCTTTCAGGAATAGAACTAGCGCGCAAAACGGCGAATCTTGTTGACCATATCTACAAAGGATAAAACCAACAATTTAAAATTACCTAACGCGCTTGCGCTTAAAAAATCGCTTTGAACAACACGAAAAACAAGTCAGATTGGCAGCTCTTTTTGGAGGACTACCGTTTTTATCACGAAAAGGAATTTGATTGAATTACTTACCTCAATCACTGCCTCAACAGCTATCCTGACTTTGACATTTTAAAGTTCATTCGCAAATACGGTCCGGAGTGTGAAAAGAGCTTTTTATCACTCCAAAGTAAAACTAAGGCCGATGTTTATGGCGTGTTCACTAAGCAAATTAAAGCTGGCAGTGTTAACGAAGTGTTAGCGCAAAAATTAGTCCAACTCGATGCGCTGCGCACTAACTATCTCATTGGCGCTTTGTACTCCACCAATAAAACACAAAAGAAGCTGTTTAAGCAATCGTGAAAGAATGCTAAAAAGCAGGGTTACACAAAGCAAGAGTGGCTAATGACCTTAGTCGGTTTGCCGTTTGAAAAAGGGGAATATCATAAGCAACTGTATGCGCATTCCCGTCAAGAGATTTTGGATCTAGTGGAAGCGGTCAAAAAGCTGTATCTCAGACCAGAAAAGGATGACAAACTGGAGTTTGCTGATTCTTCCAAGGTAAGTGAGTCTAAGAGTATTAAGGTAACCAATGCAGTAACCTTACCAAGTGATGATTTAGATAAAGAGCTGTTTGAATTTAGCGGGGAAGGGGGTGATGAATAATGCCAAAGATTGAAGTTAAAAACGATGATTTAGAGTTAGCTTTAAAAAAGTTTAAACGGGTTTCGCTAGAAATTCGCCGTTTAGCACAGCGTCACGAATACCACCTTCGAAAAGGAATGCGCTTGCGTGAAAAGCGAAAGATTGCGCAGAAGAAACGCCGTAAGTTTCGTAATATGGTCTAGTTACTAGCACCTTACCAGTTTTATATGGCTAACGATAAAAAGGATAAGATTATTTTGAGTGGTGAGTTAACTAACCATCGCTTTAATTTTACCAAGGATGGGGAAAACGGCTATAGTGCTTATGAAGTAGACCGTTTTCTAGATCAGCTAGTTCACACTTTAACCCATTACGAAGCGCAGCGTAACCGTGAGGAGGAGATGAAAACGGCTTACGAAAAGCTCTTTCAAGACCGCGACGAAATTTTAAAGCGTTGCTCCAAACTAGAAGCAGAGCTCAATAACTTTTATGAAAACGGTTATTCCAACCGTGTGTTAATTAGTCGGGTTCAAGCCTTGGAAAACAAGATCGAAAGCCTCCCTAGTGGTCAAAATGACCGTCTAGAGCGCATCGAAAAGTTGCTAAAAAGGGTGATTAAGCACTGGACTGACGGGGAGGATTTATCCTATGGTGACTTCGATGATGATTTCTTTTAAAAATGATTCTAGGCATAGGGATTGATTTAGTCGAAATCAAACGCTTTGAACAATTAGCTAGACAAACTGATAACTGTTTTGCGAAACGCTTGTTGACTTCAACGGAGTACGCGCACTATGCGAAGCTGCGTAAAGATAGTGAAAAGTCGAGTTTTTTAGCAGTCCACTGGTCATTAAAAGAAGCGATTTATAAGGCTGTTAACCACATCAAGCCATTGTTTAGCCAACTGGAAATTACAAAGAAAAACCAACGTTATAACTGCCAGATTGACCCTAAGATTGAACTTTTGCTCTCGGTTAGTTACAGCAGTAATAACATTACTGCCATCTGTTTGGCACAACAAACACCATGAAAAAATTAACACAAGCTTTTTTGCGTTTTTGTCTGAGATTCTTACAACTGCTCAGTTTAGTTTTGGTTTTACCCGTTTTTGTGTTAATGCTCATCTCTAGCCTAATTAGTGCTAAAAATTATGAAAGCATCCCGGAAAACTATCCCCCAGAAATCCGCTTTAAGAAGGTGTACCGCTTGGTATCACTTTTCCTCTACATTAAAGGAGTTAAAGTGGTTATTGTTAACCCTGAAAATGTGCCTAAAAAGGCTGTTTTAGTGGTAGCTAATCATAAGTCCAATTTAGATCCCCTCATCCTCATTAAGGCCTTTGGTAAAACTGAAGGGGTACCACCGTTAACCTTTATTGCCAAGATTGAACTGCAAGATACCTGACTGTTCAAGATTATGAAGCTGATTGACTGTGTCTTTATTGATCGAAAAAACCTGCGTCAAATGGCAGCATCCTTGGAACAACAGCAGCAAATTATCCGCCAGGGCACTGCTCTGTGTGTTTTTCCTGAGGGTACACGGGTACTCTCTAGGCAAATTGGTGAGTTTAAATCGGGCGCTTTAAAGGTCGCTTACAACGCCTTTGTGCCGATTGTGCCACTAACGATTGTGGGTAGCATGGGACACATGGAGTCCAAAAAGCGTTTACAAAAAGCCCAGGTAGAGCGCGATCGTGGTTATAAAATCCAGGTCATCTTTAACACACCTATTAACCCAATTAACTTTAACCAGATTGATTCGCAAAATGTGGCTAATAATGTCTGGCGTGAAATTAGTCAAACTTATGCGCAATATTGCCAAGACTAGATGATCACGTTAATCTGGTGCCAAGATAAACACTTCGGGATTGGGAGAGACAACACCATTCCCTGAAAGTTAACGGAAGCCAACCAACACTTCTATAACACTACTAAGAACCAAACGGTTGTAATGGGTTACAACACCTTTCAGGAGTTAGGTGACAAGCTAACTGATCACAACGTGGTGGTGTTGAGTAAAAAACACTTTGAAGAATTGCAAAACAACACCAACATTAAGGTGTTCAATTCAATTGAAAAGCTCTTGCAACACCACTTTAACCGTGATTTGTATGTGATTGGGGGCAAGCAAATCTTCCACCACTTTATTGAACTGGCTGATCGTTTAATTATTTCGGTTTTACCAGTGGACTTTAAGTGTAACCTTCGCTTAAAGTTGGGCTTGGACAGCTTTGAATTAATGCAAGAACAACAACACAGCCAATTTAAGGTGCAGTACTGACACAAAAAGCACCCCGAACGTTTATCGTTTAATGTTTTTTTGGAAGATTACAATGGGACTTTACCAAACTTGCTGGAACTTTTAATTGATAAGAAGTTCAACTTGCACCAGGTCGATATTGCCAAAATTACGACACAATACCTCCACTTAATTAACACTAACTTAAATAAACAAGCAATTGAACCAATTACCGACTACCTTGTCATTACTAGTCGAATTGTGGAGCAGAAGGCCAATAACTTACTGCAAATTAATGATATTGCCCTAGACTCTGACTTTTTAGACAACAAGCTGCGTGATAAACTAGTAGCACAGTTAGTGGAGTACAAACGTTACCGTGAATCACTCGATGACTTTGAGAAGCTAAGGATCAATCGCTTAGCTTACTTTTCCAAAGATAATGACTTTAACCGCTTTATCCAAACAGTTGACAAGAGTAATACAGAACCCGTGAAAATTGAGGACGAGCTACCCAATTATGTAAGTGTCTTAAAGTTGCATCACGCCATGAATAAGCTAATGCAGCGCTGAAGAGCCCAGTTTTTAGCGAACAAAAACATTTCAATTCAGGAGCTTTCAATTGAACAAGTCCAAGCGGAAATTTTGGCTACAATTAAGCAATTTGGCTATCATAGTGTTTCGTTAAAACGGGTACTGTTAAAGGTCAACCACCACATTTCCTTAATGTATTTCATTACCGCTTTTGTCGCTTTATTGGTGTTAATTAACAACCAGATTATCGACATTGAGCAGACTAGCTTTGATGATGAGCTTTACATCTGCTTGTTAGACAGTTCGCGGATTAAACAGCTCCAAGAAACGCCAGAAGCCATGGTGGAACGTGCTGTGAAACAACGCCAGGAAGCACAGGAATTAGCCCGTCAAGTAGCTAGAGAAAAAGCAATTGCCAACGCGCAGAAACGCGAAGCTTACCTAAAGGCAAAATATGGCAAGGATTACTTAACACGTGAACAGTTTTTAAAACTGTCTCCCGAAGAACGTGCTGCCCATGTGGCTAAAATGAAACAATTAAAACTAGTAAAGAACGACAATGGACGCGACAATTAAGGTAACTAAACCCGTTTTAAAACAAAAGGATAGCAGCGCTGCTAATTTAGTTGCTGCCATTTATGGGTTGTTGTTTGTAAGTGGCGAAAAGGGCTTAACGTTAGCGGAATTAAACCGCGTATTGCGCAAGGTGGGTTTGGAAAAGATTAAGGCCGCTTTAGTGCAGTTAGAGCGCAAGTTAAGTTTAGATGATGAAAGCGGGATTGAAATTAAAAAGTTTGGTCACAGCTTTCGCTTGGTCACCAAAATGGAGATCAAGGACTTTATCCACCGTTACCTACCCAACAAGATTAAAAATCCGCTCAATTCCAAAACAATGGAAGTGTTAGCAATTATTGCTTACAACCAACCCTGCACCCGTCCGCGCATTAATGAAATTAGGGGTGCAGATTCCTTTCAGATTGTCGATGACTTACTAGAAAAGGAGTTAATTGTTGAATTGGGCCGCAAAGACACACCTGGTCGTCCCTTTATTTATGAAGTGTCGCCTAAGTTCTATGATCTGTTTGGGATTAACAGCTTGGATGAACTGCCTAAAGTCGAGAATTTTGATCTCGACAAATTCCGCCAAGGTAGCTTTTTTGACTCTAACCGTTACGGGGATGATTAAGGTTATATAATTAGCGCAAATGAGTCCAAAAACAACCAAAAAAATTGCCATTTTAACCTCTGGTGGTGATGCTCCAGGGATGAATGCCACACTAGTTTACCTAACGCGTTATGCCACTAGTTCTGAAATTGAAGTTTTCTTTGTGAAAAACGGTTATTATGGGCTGTACCATGATGAGTTAGTACCCGCTCATCAATTAGATTTGTCAAACAGCTTGTTTAGTGCTGGCACCGTGATTGGTAGTAAGCGCTTTGTCGAATTTAAGGAGTTAAAGGTCCGCGAGCAAGCAGCACAGAATCTTAAAAAGCGCCAAATCGATTACCTAGTGGTTATTGGTGGTGATGGTAGTTACATGGGCGCTAAATTGCTAAGCGAGTTGGGCGTTAACTGTTACTGTTTGCCGGGTACGATTGACAACGACATAAATTCGAGTGAGTTTACAATTGGTTTTCTCACGGCTTTGGAGTCGATTAAGGTCAATGTCCAGGCCGTGTACCACACTACGAAATCACATGAACGGGTCGCCATTGTGGAGGTGATGGGTCGCCATTGTGGTGATTTAGCCATTTTTGGTGCCTTAGCCACCAATGCTGACTTTGTGGTGACTCCTAGCAATAAAATGGATTTAAAGCAGCTAGAAAGTGCTGTCAAAAAGATCTTGCAACACCAAAACCACTGTGTGGTGATTGTTAGTGAAAACATCTATGGCTTTGATGGTTATCCCTCACTTACAGCGATCAAACAGCACTTTGATGCTAACAACATGAAGTGTAACCTGGTGTCCTTGGGTCACACGCAACGCGGATTTGCCCCTACCAGCCTCGAGTTAGTCCAAATTAGTTTAATGGCGCAACACACGATTAACTTAATTGGACAAAACAAGGTTAACCAGGTCATTGGTAATAAGGCCAATGTGCCCGTTAACTATGACTTTGATCAAGCCTTTAACATGCCACCGGTTGATCGCAGCGCTTTAATTGCAGTCATTAATAAAAATATTATTTAACTGATATGATTCACCACCTAAAACGCACTAAGATTATTGCCACCTGTGGTCCGGCTTTAACTAAAAAGCTATGAACGCTAGCAATGTTAGATGACCCAGCTTACGCTGCGATGAAAGCAGAAGCTTACGCCAACATTGAAAATATTATTAAAAACGGCGTAACGGTAATCCGCTTAAACTTCTCCCATGGTAACCATGAAGAGCAGGCTGTAAGGATTAAGATTGTTCGTGATGTGGCTAAAAAGCTCAACCTCCCGGTGTCAATTATGTTAGACACCAACGGTCCGGAAATCCGCGTCTTTGAAACGGCCCCTGAAGGGTTAAAGATCCTCAAGGACAGTGAGGTGGTTATTAACACCACCACTAAGGAAGTGGCTAAAAATAACCAGTTTAGTGTGAGTGATGCTAGTGGCACTTACAACATGGTCAACGATGTTAAGGTGGGACAAAAAATCCTCGTTGATGATGGTAAGTTAAGCTTAGTCGTAAAGCGGATTGATACGAAGAACAACCAGGTTATTTGTGTTGCCCAAAATGACCACACTATCTTTACCAAAAAGCGCTTAAACCTCCCCAATGCCGACTACTCAATTCCCTTTTTAAGTGCTAAAGACCTACGCGACATTGACTTTGGTTTAACCCACCAAATTGACTACATTGCCGCTTCCTTTGTCAATACAACGGAAAACATCAAGCAACTGCGTGACTACCTCGCTTCTAAGAATGCCAAGCATGTCAAGCTTATTGCCAAAATTGAGTCCAACCACGCTTTAAACAACATTGATGGGATTATTAAAGCGAGTGATGGCATTATGGTAGCACGGGGTGATTTGGGCTTGGAGATCCCATATTACAAGGTGCCTTACTGACAACGGTACATGATAAAAGCGTGTCGTTTCTTTAACAAGCGTGTTATTACCGCAACTCAAATGTTGGACTCTTTGGAAAAAAACATCCAACCAACTCGCGCTGAAGTTACTGATGTGTACTTTGCCGTGGACCGCGGCAATGATGCCACCATGTTAAGTGGAGAAACCGCAAATGGGGCGTTTCCCCTTAATGCTGTGTATGTAATGAAGATGATTGATAAACAGTCCGAAACGTTCTTTGATTACCAGTACAACTTGAACTATTACATGGCCAACTCCAAGGCCCGTCACAGTGAGTTTTGAAAGCAGGTGGTGTTACCGTTAGCACAAAAAACAGCACCAAAACGCAAACTGATTAACAGTGACTTTAAATACGACTTTGTGGTGCACGCCACCAACAACCTTAATGAAATCTATGCCTTGTCCAACGCTCGCTTAGCGGCTGCGGTAATTATCTTGACCAATGACCCCCAAGTATACACCGGGCACGGCGTTGATTATGGCATCTTCCCTTACTTAATTGACCAAAAGCCGCAAAGCTTAAGTAAAGCTGAGTTTAAGAGCTTAGCTAATGTTGCCATTAAACACTACCAGCAGCATGGGGAAATTAGTCAATTAAAGCAGTGTTTGGGAGTATTTCACAATAAGATCATTAGCTTATAAGCGCTAAAAATTAATTAGAGTTTGTAAAATATTCTACACGGTGCCTTAGCCAAGTGGATTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCATGCACATGAACAATAAATCCACTACAAGGTGAGAACCCTTGCATGTGCAAACGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCATAATGGCTGGATACCTCAGTTGGTTAGAGGACCCGGTTCATACCCGGGTTGTCGTGAGTTCGAATCTCACTCCAGCCACCAAAATCATTAAGGATCTATAGCTCAATGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCGAGTCCTGTTAGATCCACCATCTGGTTGCTGTGTTAGAATAGTACTTGCCGCATTCACCTGGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTGAAAAGCACGCGAGGGTTCGAATCCCTCAGTCTCCGCCAAACCACATTGATCGCGGGATAGAGCAGTCGGTTAGCTCGTCAGGCTCATAATCTGAAGGTCGGGGGTTCGAATCCCTCTCCCGCAACCACGGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCACGGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCATAAAACTTTCTTACCTTACCTTTAAAACTAAGGGTAAGGTTTTTTATTGCTTACCACCTTTATTTAAGGCAGTACAACAGCTGTAAATTAGTTGCCCAATTCTTTTTGCAATCAAGTTCACTTTGCCTTTTTTTCTTGGGCTTTTGTGTGGACGTGTAACAATTAAAGCAGTAAGCTTAGCCTTATTACATGTAATATGAAGTACAACATCAACGTTCATTCCGAAATTGGTCAACTGCAAACCGTTTTAGTCCACACCCCTGGTAATGAAATTAGACGGATTTCACCAAGACGGTTGGATGATTTACTGTTTTCAGCAGTAATAGAACCAGATACTGCCATCCAGGAACACCAAACCTTTTGTCAGTTACTGCAAGAACAAAACATTGAAGTGGTGCAACTGACTGATTTAACAGCCACCACCTTTGACAAGGCAAATGCTACAGCGCAAAACCAGTTTATTGAAACTTGACTAGATCAAGCAGAGCCCAAGTTAACCCCAGAACACCGTAAAGTAGCGAAGCAGTACTTACTGGAACAAAAAGCAAAATCGACCCTCAGCATGGTACGCTCGATGATGGGTGGGATTGATAAGCGTAAGGTAGCTGCTGCTAACACCATTAACGGTGATTTTTTAGTAGATCCGATGCCTAACCTTTACTTTACCCGCGATCCGTTTGCCTCGATTGGCCATGGTATTAGTATTAACCGGATGAAGTATTTAACGCGGCGGCGTGAAACCTTATTTGCTAGTTTTATTTTGCCAACCACCCCGATTATTGCCGCACGCAAATTTACTTTAAACCAATTGACATGGGCACAATTGAGGGTGGTGATATTTTTGTATATGACCAACAAACAGTGGTGGTGGGGTTGAGTGAACGGACAACCGAAGCTGCCATTAATGTTTTAGCGAAAAAGATTCAACAAGACAGTAGCACTTCCTTTAAGCGGATCTTTGTCATTAACGTTCCACAACTACCGAACTTAATGCATTTAGACACGTGGCTGACAATGTTAGACCGCAACAAGTTCTTGTACTCCCCGAACATGTTGGCGGTTTTAAAGGCGTGGCGGATTGACTTGACTGATCCGGCTTTAAAGTGAAATGAAATAGCTGGTGATCTTTCAACCATCTTACACACCATTATTGGGCAAAAACCTATGCTCATTCCCATTGCCGGAGCGGATGCTAACCAAACCGAAATTGACATTGAAACCCACTTTGATGGCACCAACTACCTCACCATCGCTCCATCGGTAGTAGTGGGGTATGCGCGCAATAAATTAACACACCAGGCCTTGGAAGCGGCTGGGGTTAAAGTAATTGCCTTTAAGGGGAACCAGCTGTCTTTGGGAATGGGTTCTGCACGTTGTATGTCGATGCCATTAGTACGCAAACCACTTTAATTTTTCTTTATTTACTTATACAAATTTATGCCAATTAACTTAAAAGGTCGTAGCCTTGACTCTGCTTTAAACTTTACTACTGCTCAAATTAACTATTTAATTGACCTCGCCATTGATTTAAAACGCAGTAAATACCAAGTTGCACATCCAGAACCGTCCCTTAGTGGGGAAGAACATTGTTTTACTGTTCCAAAAAGATTCTACCCGTACCCGTTGTGCCTTTGAAGTGGCTGCCTTTGACTTGGGTATGGGTTGCACTTACATCGGCCCTTCTGGTTCCAATTTTGGTAAGAAGGAATCGATTGAAGACACTGCTAAAGTGTTGGGTTCAATGTATGACGGAATTGAATTCCGTGGCTTTAAGCAAAGCGATGTTGATGCTTTGGTAAAGTACTCTGGTGTTCCTGTTTGAAACGGTTTGACTGATGCGGAACACCCTACCCAAATGCTAGCGGATTACATGACGATTAAGGAGTTGAAGGGTGATTTGAAGGGGCGCAAAATTGTCTTTGCTGGTGATATTAAAAATAATGTGGCACGCTCGTTGATGATTGGGGCAGCTTTTGTTGGTATGGACATAGTGCTAGTAGGTCCTAAAGCACAGCATGAATTAGTTCAAAATGGTGCTGGGTATAAAGAGGTCTTTGAGCAGTGTCAAGCATTGTTTCAACTCAATGGTGGTAGTGTTAGCTTTAGCACTGATAAGCTGCAAGCGGCTAAGAATGCTGATGTAATTTACACCGATGTGTGACTGTCGTTGGGTGAAGACTTTAGCTTATTTGAAGAACGGATTCAGGAGCTGGGTCAATTTCAAGTTGACATGGCTATGATCAAAGCAGCTAAGAGTGATGTGATCTTCTTGCACTGTCTCCCCGCTTTCCATGATGATCACACTAGCTTTTCACTGGAAATTAAACAAAAGTTAGGTAAAAAATATCCGGTGGTGAAAACGGGTGCGATGGAAGTGACTGATACGGTGTTTCAGTCCAAGCATAACATGGCCTTTATCCAAGCAGAAAACCGCTTGCACACGATTAAGGCCGTTATTTTAGCTACTTTAGGGTATTAAAGATGCAAAAAATTGTCATAGCATTGGGTGGTAATGCATTAGGTAACAATCCCACCGAACAAAAGGACTTGGTACAACTACCAGCACGACAGGCGGTTGCTTTGTTAAAGCAGGGTTATCAGATATTATTGGGTCATGGTAACGGTCCACAGGTTGGCATGATCTACAACGCCTTTAGTGCGGCCAAACAAGTCAACCCTAATACACCCACTGTACCATTTGCTGAAAGTGGGGCAATGTCTCAGGGTTACATTGGACTGCATTTGTTAACAGCATTGTACAATGAGCTGCTACACCAAAAACTACCCCATCAAGCCGTGTACTTTTTAACACAAACCTTAGTAGAAGCGAGCGATCCTGCTTTTCAAAACCCAAATAAACCAGTTGGTCCGTTTTACAACACAGAGGAAACAGCACGCTCAGCTAATCCAAACAGCACCGTGGTGGAAGATGCAGGGCGGGGTTGGCGTAAAGTAGTAGCGTCCCCCAAACCAGTTGATGTTTTGGGAATTGATGCCATTAAAAGTAGCTTTAACCAAGGCAACTTGGTGATTGTTGGTGGCGGTGGCGGTGTACCCACAATTAAGACCAAAAGTGGTTATGCAACGGTTGATGGGGTGATTGATAAGGATCTAGCATTGAGTGAAATTGCCATTAAGGTAGAGGCTGACCTGTTTGTTATTCTCACTGCCGTGGACTTTGTCTACATTAACTATGGTCAACCAAACGAGCAAAAGCTAACCTGCATTAACACCAAGGAAGCTAAGACCTTAATGGCCGCAAACCAGTTTGCTAAAGGTAGTATGCTCCCCAAGGTAGAAGCGTGTTTAAACTTTGTCCAGTCGGGTACCAATAAGACGGCCATTATTGCCCAATTAGACCAATTAGCTGCGGCCATTGCGGGCAAGATTGGTACTAAAATTGTCAAGTAGTTATGAAACAACAAAAACCCAAAATTAGCTTTATAGCAGCGATGCTAATTGTTATCGGCTCGTCCATAGGTGCTGGTATCTTTTTTAAATCCAGCACTGTTTTGGAAAATTCCCAGGCCTCTTTAGTGCTAGCGATTTTTAACTGGTTGGTAGCTTCAGTGGCAGTGATTGCTATGGCCTTGGCATTAATTGAAATTGCCAGTGTTCGCAACGATAATCTCTCGATTATTAGCTGGGTCAAAGTGTTTAACCGGCGCTGGTTGTACCATGGTTGTAAGAATTTTATGACCTATTTGTACCTACCGTTGACCTTCTTCTTTATGCCGCTGTACTTTATCTGTTCCATTCAAGATGGGTTTCGCGGTTTGTTGGGACTGGAAACCGGAGCGCATTTCAATACTAGTGTGGACTGGTTAATCTGGTTAGCTTTAGCACTTATTATTACCACTTACTTCTTGACAATTCCACCACTGTACGCTAAGGTAGGGAACATCCAAAACATGGTGGTAAGCGCCGTGAAGTTTATTCCTTTAGTGTTTGTGCCTATCATCGGTTTTATTGTAGCGGGTACAGGTAACGGTGAATTAAAAAACGTTAAGGCATTAGTGCAACCCCCACAAATTAATGGTGCGACTGCTTCCTTTACCCAATTAGTACAAGCGGGGTATGGAATTACTCGTTTTACGGGAATTGGTGCTGGGATGGGTTCCTTTATTTCGATTGCCGCCATTTTCTTTGCGTACGATGGTTTTTACGTAACAGCTGGACTGCAGTCAGAGATGCGTGAACCGAAAAAAACACCGTGAGCTTTGTTTTTAGGTTTACTAATTACTACGCTGTTTTACCTTATCTTAGCAGTGGCACTTTCCATTAACGGTGGTTTGTTTAGTGGTATGGAAGAGTCCATGGGAAAGCTGTTTAACAATAAGCGTGCCGGGCAAATTGTCTTTGGTGTTGTTAACCTAATGATTGGCATAGGTGTCTTGGGTATTATTAACGGTTTTGCACTGTGAGCACCACGGTTTGTGGAAGACTTATTAGCCCAAGGTGATTTACCTTTTTGAAAGCAAGTGCAGGGCAGATTGAACCCCAACAAGCCAGTGGTGGGGGTAATTTACTGTTTGGTACTTTCTTTGACTGTTCAAGTGCTGTTTACGGTAATAGGTGCTTTGGCTTACCTCCCGACAGTAGCGGATTACAAAAATTACGTTAACACTGAAATTGACAAGCTCAATTCGATGCAATGGTTGTATTCGTTTTCCGATTTAATGGCTACTTGAACTTCGCTGTTTACCTTCGCCTTTATTGCCTGTGCCATCTTTGGTGCCATTGTTAATCGCAAAACCAAGCAGATTACGATTGCCAATCCAAAGCGTTATTTTCTCCCAGCGGCTTGGATTGCCGTAGTGGTTAACTGCATTAGTGTTTTTGTAACTATCATTGAGCCCTTCATTAACCTCTTTTTACTGTTTGGTTATGATGAAACAGTGGCACACACAGTGCTCGGTAATGACTTCATTGAATTGAATGAATTAGTGATAGGACGGGTAATGCTGATAGTAGTGTTAGTTTTCTTTGCCATAATTAGCTTTCTCCCTGTTTATGTGGAGGATCAATACCACAAACGTAAGTTTGGTAGTTTAGCTAACTATCAGCAATATGTACAACAACATTTAGCACACTCCACCATTAACGGTTAATTAGTTTTGTAAGGCTTGCAAGCTTTGCCGTCATTTTCATTTAAATAGCATTTATTCATTTGCATTTTTTTAGATAAATTAAAATTAATGATATAGTGTCAGCTTTGCTGTTGGCACCTTTTAAAGACAGTAAATGGATATAAATAAACCAGGTTGAAATCAGTCTGACCAACAAGCTACTGCATACGATCCCAATCAGCAGCAGTACTATGGTGATGGTAGTACTTATTACGACCCCGACCAAGCAGTTGATCCCAACCAAGCGTATTACCCCGATCCGAATACCTATCCTGATGCAGCGGCTTATTACGGCTATGGTCAGGATGGTCAAGCATATCCACAAGACTATGCTCAAGATCCCAACCAAGCGTATTATGCCGATCCCAATGCTTATCAGGACCCAAACGCTTACACCGATCCTAACGCCTATGTAGATCCCAATGCTTATCAGGACCCAAACGCTTATGTAGATCCTAATAACTACACCGATCCCAATGCTTATTACGGCTATGGTCAGGATGGTCAAGCATATCCACAAGACTATGCTCAAGATCCCAACCAAGCGTACTATGCCGATCCCAATGCTTATCAGGACCCAAACGCTTACACCGATCTTAACGCCTATGTAGATCCCAATGCTTATCAGGACCCAAACGCTTACACCGATCCTTACTATGTTACTAGTACCGATCACAACGCTTACTATGGTCAGGTCGATAATGTACCTGCTTTAGAGGCGAGTGATCTCGCTTATGAGGTAACACCGCAAGAGCAAGCAGCGGAACAAGAGCTGTTTAGCGAACCGGAAACAAAGGTAATCCGTGAAATCCACGAATTTCCCTTTGAGAAGATTCGCTCTTACTTCCAAACGGACTTTGACAGTTATAACAGCCGTTTAACCCAGCTCAAAGACAAGTTAGATAACGCCATCTTTTCGATGCGCAAAGCGATTGATACTGTCAAGGAAAACAGCGCTAACTTGCAAATTATGAAGCAGAACTTTGAACGCCAACTCAAAGAACAGCAAACCCAACGGCTTACAAGTAATACTGACGCGGAAAAGATTGGTGCTAAGATCAACCAATTAGAGGAACGCATGCAACGTTTATCGCGCACTATGGAAAGTGTGGAGTGAACCAAAAAGGAGCCACGTCAAGAACAGTTCGATCCACGCTTTGTCGATCCACGTAACTTTAATAACTATGTAAACAACACTGACACAATGATGTCAATGTTTGAAAAGGTATTAATGATGAACTTACTGCGCAGCACCACTCCAGTCCAACCTCCAGTGCAGTACTTTACTCCTCAACCCCTTACAGCATCGCCACGTCCAGTGTATGAAGAACCGATCTCCGCTTCGTTCCGCCGTCGTGGTTACCGTGGTGATGAATTTTACGAATAAACACTGGCTCTTTTAACCGATGAATGATACTGACAAGAAGTTCCCCTTGCAACCAGTTTATGACACTGGGTTTGATGATGGGTATTTACAGCGTGATTATGAAAAGCGTTTAGAAAGTGCTGCTGCTAATGATGCACAAACAGTGGAACTACAAACCCAGCTGTTAGCAGAGATTAAAAACCTTGAAAACGAAATCAAGGCCCTAAAGGCGCAGGAATCACGCCAACCCGATCCGCACAACAATGCCCGGATTCAAAGCTTAGAAGCTAGCTTAAACCGTTTGGTCAATGAGTACAACAACTTTGAATTCCAAAAGAACTATATGGTTGATCGGGTAGCAGAGTTAAATAACAAAGCGCGTTTTTTTAAGGACGAATTAAAGCGTCTCCAACAAGAAAACGCCGCTTTTGTTAATTCCCGTTATGCCAATTGAGCTGACTTTCAATCCAACTATCAGCTCAAATTAGACCAGTTTCAAGCGTTAATTGACCAGCAAAACCAAACAATTAAGCAGCTCAACGAACAGATTGCAGCTAACCAGGGCTTAATTGATCAAAACGTACAGCGCTTACAGCAAAACCACAGTTTAGACCAACAAGAACGTGACGCCTTACTGTATGAGGTTGACCATTTATACAACGAACTGTACGAACTTGAAAACCAAAAACGCTTAGTGGGCATCGAGTATGAAGCCACATACCAGGACTTGGTGAGTGCGGATGCGGAGTTGCAAAACGTTTATGAAACAATTGCCCAAAACCAGGCCAACTTCCAAAAGCAGTGTGATGCTTATTGAGCACAGCTCAAGCAAGTAGAACAGCAAATTCAAACGACAAAGCAAGAATTGGTTGATGAAGAATCGGCACTTAAGGTGCGTTTAAATGACGCTGATTTTTACATTAACTCACGCTTAGCAGAATTAGATGATTTAACTAGCAAGATTAACGAACGTGATTTTGTTTCTAAAGAACAAGCTCAAGACGTTAAAGCTAGTTTAGCTAACCTCACTAAGGAAAAAGAACGTTTGAGCGCTGAGAAGGATAGCTTTGAAAGGTTAAGGAACACCGCTTTAAACGACATTAACCGAATGGAGCAGGAAAACGCGTTGTTTGCTAAACACTTAGAACAGCAACAATACGAGTTTGAAAGAAAGCAACAGGAAAGCTTATTAAAGCTAGAAACAGAGCATAAACAGCTCCAAAAACGAATTGGTGAGTTCAAAATCGAGAGTGAAGCTAAAAGTGAAGCGTTACTGATACAAGAACGTGAACTGTTGGAAAAACGCCGGGAAATTGATGATTTGTTAACCCAAGCCAGCTTGGAGTATGAACAACAACGCCAGACGAATCAGGTCTTAAAGGAAAAGCACCGTCAAGTCCAACAACACTTCCAAAACCTCGTGCATGCTAAAAAGAAATTGGATCAAAAGCGCCATTATTTAGCGGAACAAAAGCGGATTGATGAAGAACAGATTTTTAAGTTAAAGGAAAAGATTGCCACTGAACGCCGCGAATTGGAGAAGCTTTATCTAGTTAAAAAACAAAAGCAAGACCAAAAGGAAAACGATTTACTGATTTTTGAAAAACAGTTAAGGCAGTACCAAGCTGACTTTGAAAACGAAATAGAAGAGAAGCAAAACGAGCTGTTTGCATCCCAAAAATCACTCCAAAAATCGTTTACACAACTCAAAAATAAGGAAGCGGAATTAAACCAAAAGGCACAAAAGATTGCAGAGGATTGAGCACATCTCAAACAAAACAAACACCACCACGCTGATCTAGAAATCTTTCTGGAGGGTGAATTTAACCACTTACAACAAGAAAAGCACAAGCTGTTGGAAGCACGTACGCAGTTTGACAACCGTGTCAGTTTGCTCTCAGCACGCTTTAAACAAAAACAAGCAGAATTAGTTAAACAAAAACAAAGCTTGGAACAACTCACCGCTGCCTTTAATAAAGAGCAGGAAGCGGTAGAGCGTGATTGGAAGGATCGCTTAGCTAACTTAGAAAAGCAAAAGGAAATGCTGGGGGATAAGGTACACCAGTTTGATGAAAATTCGCTCAATATTTCCAAAAAGCTAGCGGAGCGGGAGCTAGCGATTAAATTTAAAGAAAAGGAGCTGGAGGCTGCACAAAAACAGCTAAGTTTAGACAACAACAATAACGCTGGGTTAAAGTTGCAGTTAGATAAATTGAGCGAATCACTCAAAACGGAACGCCTGGAATTAGAAGCATCTAAAGAACGTATTTTGGATTTTTACGATGAATCATCACGCCGAATTGCAGATTATGAAAGCGATTTACAAGCACGCTTAGCTGAGGTTAAGACACTAGAAAAAAACCAGCAGGAAACAGCGGCTAAAAGTGAGCGCGAACTCAAAGTAGCCTTAGAAAAGTTAAACCAAGCAAAAAAGGCTTTTTTACAAATTCGCAAACAGCAATTGCTCGAAATTGCTTCTGTAAAACAACAACTAGCACAAAAAGCTAATTTGTTGAAAAACCAACAAGCAGAGCTAGATAAGCAAACAGAAGAGTTAGAAGCAGCTTTTTTAGAGCAAGATACGGACAAAAAGGAGTTGGAAAAAGCACTCCACAGCGTTAAGTCCAAGCAAGAATTATTAGAGCGCGAACGCAGTTTTTTACTGCAAAAACAACGTGAATTTGCCGAACATGTAGCCGGCTTTAAGCGCCAAGTGCACTTTAAAACAACGCAAATGCAGCGTTTAAGTGAGTTCAACAAACAACAACAAAGTGAACAGATAAAGCGCGAAACGGAGCTCAAAATTGCTTTTGCTGATCTAAAAAAGGATTACCAGCTGTTTGAATTGCAAAAAAACCAGGAGTTCCAGCAAATCGAACAAAAGCACAAAGAACTAGAACTATTAGCACAAAAACAGGCCGAACTCAAACAGGAGTTAGAGCAAAAAGCAACAGCTTTAGCTAGCCAAGATCAAGACACAGTACAGGCCAAGCTAGATTTAGCGCGTCAACAGCATGAATTGGAATTGAAGCAAAATGCCTTTAACCAAGCTAGTCTTTCGCTCAACAAACAACGCGAACAGCTCACAAACCAAGTCAAGGTATTGCACGGTGAGTTGAAAAAACGCCATGAAAAGCTAACTTTAAAGGATCGTTTACTAGCTGAAAAGGAAAAGGATCAGCACAAAAAAGATGCAGAAATTAACCAACGCTTTAAGCAGTTTGAAAATGAATACGCTGATTTTGACCAAGCCAAAAAGCGCGAATTGCAAGAGTTAAACCAAATCCGTCGAAACTTGGAGCAGAGCAATGCATCACTGCTAAAGAAGCGCAACCAACTAACGCTTGATTTTGCTTTACTACGTAAAGTGCAACACAATACGCAAACTAACCGTGTCCAACTCAACACGCAAATTAAGGAGTTTTTACTAGAGAAGAAGAACTTTCAAAAGGCGAGTGATGAAGCCGCTTTACAAAAAGCACTGTTAATTAAACGCTTACGTAGTTTTGCTTCTAAATTACAGTTGCAACGCGAAGCTTTAGCGATTCAAAAACTGGAATTTGATAAGCGTGATGAACAACAAAAGAGTGAGATTAACAACGCTAAGTTACAGTTAGAGCAGTTCAAACTAGAAAAGCAGAACTTTGATGAAGCGAAACAAAAGCAGTTAATTGAGTTTAAGGATCAGTGCCAACGACTTGATGTTGAAAAACGTCTGTTAAAGCAAAAGTTAGTGCAGCTCAAAAACCTATCTAAGAGCTATCTTACTTACAAAAACCGTGCTGATCTGTCCCAACAACAGCTGCAACACAAGTATGCTAACTTACTGGAGTTGAAGGAAAAGCTGCAAACCGCTAAGCGTGCCTTGGACAAAAAACACCGTGCCATTTATGGCAAAATGGCACAGTTTGTCAGTGAGCTCCGCCAAGAAAAGAAGCAGCTTTTAAGTGCGCAAAAACAAGTTGATGACAAGTCACGTTTACTGGAACAAAACCAACGCCATTTACAAAACCTTTCCAGTGAAACCAAAAAGAAACGCCAGTCTTTAGAGCATGACATTAATAAGTTTGATCAAAGACGCAAGGAAGCGGTTTCTTCCATTTTAAACTCGCACAAAAAACTCAAGCAAAAAGAGGGAGAATTGCAAGGCATTTTGCAAAAGCTTAGCTTGAAAAAAACACAAATTGAACAAGAGTTTTCCAAGCTTTATCAGCAACGCGAAAAGTTAGATCGCCAACGCACTACGCTCTCTAAACTCCACAGAGAGCTAAAAGCCCAAAATGAAGCTACGGCACACAAAAATCGTGAAGTTTTAGAGATTGAGAACTATTACAAGAAGGAGTTGCAACGACTCACCACGGAAAAGAGTGAGTTTGATAACAACAAAAATCGCTTGTTTGAATACTTCCGTAAAATTCGCAATGAAATTGAAAAGAAGGAAGCACACATTAAAACCGTTTTAGAAGAAACACAAAAAAAGCGTCACCTAGTCGAAACGGAAGCGGTCAAACTACATTTACAAAAGCAATCTATCATTTCTAAGGGTCAAGAACTTAAAGAAATCAAGGAACGGGTTAGCCGTGACATTAGCCACACCAACAAACAACGCGAAGAGTTAAACAGCTTATTGCACCAAAACAAACTGTTGCAAAAGAATTTGGCTGAGCGTGAACGTGAAATTAATAATAAGGATTCACTGTTAACCCAAAAGATTCAAACAGCTAAACAAAAGTTAAGCGAAAAAGAAGCACGCATCTTAAAGCTGTTGGAAAAAATGCGTGCGGTAGAACAGCAATACCAAGCGGAAATTACCCGTTTAAAGACACGTAATGCTGATTTGGAGAAGAATGACAATAAGCACTTATTTCCACCGTTATTTAAAATTAACGGTAATGATATGAACTATCCTTACCCATATCCTTGATTTTATCCGCAACAAAAACAAGAAGATAGTTCAAACCAAATTCGCCATCTTTTTGAACAACAGTTGCAATTTATGCAACAGCGTTACGAAAACGAATTAACTGAGTTGCGTCGCCAACGTGCTTTATTGGAAAAAAAACTAGACCAAATTCAACTCGAATCACAGTTAAGCGCGAAGAAGAACGACTTTGAAAAGGTTGAGCAAATGATGCAAAAGTTGTTGGAAAAAACTGAGCAAAAACTCAGTGCCTTTGACCAAAAAATTAATGCTTTGGCAGAACAGATTAACACACAAAAAGCAGAGCACGCTGACAGTGAAAAGCAACAGTTGTTACTAAGGATTGAACAGTTAGAAAAGCAAAACCTAGCTCAAGCTGTTCAAACACCCCAACCAGTACAACCAGTGGTACAAGCACCAGCTGTAGTACCCCAAGTGATCCAACCACAGGTAGTGCAGTCCCAACCAGCTTTTTTAGCAACGCAGCAAAGTATTTCTAAACAGCAACAAATCGCGCAATTAAACGCTGAGATCAATAGTATTAAAAAGCTAATTGCCCAAAAAGCAGCTAAATAAGGAAGAGATGAAAGATGACTAATGATTACCAACAATTAAATTATTTAGTGGAAACTGATGATGAAGCTGACATCATCATAGCTAACCTCGTCAAACAACTCAATGAATTGAAGGAAATTCTGCTTTCATTGGACAATCAAGATTTAGAAATTAATCAGGTTAACCATCGCACCACAGTGCACAATACTAGTTCTAACACTTCTAATAATAGCACTAGCAATAATAGTGCTGATGGTAATTACAACAGCAATTTCTTTCACAACTTTAGTAAAGAAACGCTGCAGACACAAGCAAAACGTGGCTTTCTTTTACTAGAACGCTGTTCCTTGGTAGGTCTACAACAATTGGAGTTGGAATACCTCAACGTATTGGGTCGTAGCTTTGAATCACATCAAGACAAAATTAACTTTTTGGTCAATTTACGTGATTTAACTAACGATCACTTGTTGGACACGGAAAAGATTGTCAGTACCCTAGAGCAGATCTTTAACGTTATTGGTGGCACGGAATACACCCCGATTCTCAATTCCTTTTTTAACCAATCACTTAACGATCCTGACCCAATTCAGCGCGAAATTGGACTCAAACAGTTTGTGGCTAACTTACGCCAACGCTTTAAAACGCTATTGCAAAAAACTAACAACTCAATTCAACAGTTAGAAGCGGAAATTCAAATTCCAACTACTCATATTAAGAGTGATGAGGTAATGTTTGGCCCACCGGACATGAACGAACGCTTAGTGTTAAATGACAGTGAAACCGATGCCATTCTACGCTCAATTGAAGCGGAGTTGGAAAGCGCTTTACAAAATAAAAAGCAGGTAGTGGTTACAGCAGTCCCACCAATAGCTGCTAACTTAGCTACCGATAGTATTAGCCAAGAAACTTTAGAACACAACCTTAATAACACTGAAACGGTTAACACCACTACTGTCACGGTAACATCAGCTAGTGAAACTCAAGTGCGTAAACCACAGATTTCATTACGCCCCATTTTTCAAGGCAACTTTCCCAAACGCTTAAGTAGAGAAGACATCCAGCGCTATGCCCACCAGTTACAGGAATTAGAACAAAGTAATGAAGGATAGTGCACTAACACTAAAACGGGTACGAATTGGAAAATTTTCCGAGTCAATGGTTGAAGAACGACCAACACTCAACCTGTTCGAAAAGGTCGAATTCAACCCAGTACCGACTGCTTTAGTGGATCAATTGCCAACTGAACCACTTGTTGAAGCAACTCTTTTAGAAAAGGAAGCAATTACTTTTGTCGATACTTACGCAACTTCGGAAGCTCATGATCAAATAGCTACCTTTGTTTTAGAACAATCAATGGAAACGGAGGTGGTTGAAAAAGAAGCGATTGAAACAGCAATAGTTGCACCAGCACCAGACTTAGTTGAAGAGAAAGCTGTTTTAGTGGAAGAGGTTTTAGTTGAACCAACTGCAACGGAAGCAGTTACAACTGAAGAAAATCAAGTTTCAACTACATCCGTAACTAAGATTAAAACTAAGCGCAGCAATACTAAAAAGGTGACATCAGAAACACTTGTGGCTTCTAAAAGTGTGAAAACTAAAAAGTTAATTACCCCTAACCGGGTAAGTTCGGGCAACGTTAATATAACACTTTGACAAGCAGACAAGAAGTCCACTAACCTAACTAAAACTAAAACCGATCTCTTTGGTAAAAAGCACCAGTTTAAGGGTCCACAGTTAATTTCTTACAAGAAGTAAGCAAAACAAAAGCCAGGCACTAACCTGGCTGTTTGTTTCGGAGAATCTAAACAAGTATTTAAATTTAAGTATTAAGTATTAATTAAACGGGAAAAGAATAGGACGATTTACCAGGGGTTAAACCTAATCGGAACCGACCGGTACTAAAGTAGTCTTTTTGTGAATTCCTTCTTTTATAGCTATAACTGCTGCCATTGCCGTTGGCATTATTAGCGTTAGCAGCTTCGCTGATGTCTAACGCGGTATTAATTAACGTACTAAAGGCGTTTACAATCATAATTACTCCCGCACTGAAGCTTCAAAATGATAAGCCTCCAACTACTTGTTTAGCTTCTGATTTTTTTAATCTCTGCATTATTGTGTCTCCATTTAAATGAATTACATAACTAAAGTAAAATGCCAAAGTTTTATAATTAACTTAACTGTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCATAATACTTTCTAACCTACCAGTGTTACCCTGGTAGGTTTTTTATTTGCTCCGTTGGCTCACGGTGGATGAAACCACCTTTGACTTACTTTTGTAAACCCAAAAAGTGGTCTTGTAAACCACTTTGGTGCCTTTTACTTTGCTGTTCCAAAGCGCTTTTTTTAGGTTTGCTGGCAGTTCTTTTAGCCAAAAAAGTTTTTTTATTCTAAGATATAAAGTGTTTTAAAGTGTCACAAAGTGGCATAAAGTGGTAAAAATGCTTTTAGGAACCTTCAACATTACCCTTGATGCAAAAAACAGGATCAGTCTGCCAGCGAAGCTGCGGGCTTTCTTTGAAGGTAGCATTGTTATCAACCGTGGCTTTGAAAACTGCTTGGAAGTGCGTAAACCACAAGACTTCCAAAAGTACTTTGAGCAGTTCAACAGCTTTCCCTCCACCCAAAAAGACACTAGAACACTCAAGCGCTTAATTTTTGCCAACGCTAACTTTGTGGATGTAGATACAGCGGGACGGGTTTTAATTCCCAACAATCTGATTAACGATGCCAAGCTAGACAAGGAAATTGTTTTAATTGGCCAGTTTGACCACTTAGAGATTTGGGACAAGAAGCTGTATGAGGACTACCTAGCCAACTCTGAGTCGTTGGAAACGGTAGCGGAAAGGATGAAAGATGTTAAGTAATCAACCCCACAAAAGTGTCTTACTTGATGAAGTCATCCACAACCTCAACATTAAAGGTGATGGTAACTATTTAGATCTTACTGTTGGTTTTGGCGGTCATAGTCAGCACATTTTAGAGCAGCTTACCACTGGCACTTTAACTGGCAATGACATGGATAAGGACAGCATTACCTTTTGTACCGAGCTGTTCAAAGACAAAAAGAACGTTGTTTTAGTCCATGATAACTTCGCTAACTTTTTTAACCACTTAAAGCAGCTCAAAGTAACGAAGTTTGACGGCATCTTAATTGATTTAGGGGTTTCGAGCTACCAGCTAGATAAACCAGAACGCGGTTTTAGTTTTAAACACGCCGGTCCATTTGACATGCGCATGCACCAAAGCGATCGCGTACCAACTGCTTTAGATATCTTGGAGAGGTTGTCTGAGGAAGAATTGACACATGTTTTAAAGAAGTTTGGTGAAATAGCCCACCCCAAACCAATCGCCAAGGCCTTAAAAACACTCATTAATAACATTAAAAATCCAACTACGGTAGAGGTAGCGGAAACAGTTAAGGCAGCTGCTAGCAACTTTGAAAAGTACAAATCACGCAATTACTTAGCTAAAGTTTTCCAAGCCATTCGCATTTACCTCAACCGTGAACTGGAAAGTTTGGAAATTGTGCTGCAACACATTCCCAAGCTGTTGAACAATAAGGGTCGTTTTCTAGTGATTGTCTTCCATTCACTCGAGGAAAAATTGGTGCGGAACTACATTTTTAAACTTACTCACTTTGTCCAACCCCCTGAACTACCCGTTAAATTAACCCCACCGTTTGAACTCATTACTAAAAAACCCATTTTGCCAACTGAACAAGAAATTAAAACGAACCCCCGTGCTCGAAGTGCCAAACTGTTTGTCATTGAAAAGAAATAACTATGTATAACCTCAAAAACATCTACGCCTCCATTACCCTTTACCCCCATGAAATTAACTTGGTGGTATCGGACAACAACAATCAGTTTCATGTCTTGTATGAAAACTCGATTGCTAACGATGAGCTTTACACGCACGCGGGCATTACTAATAAGGCAAAGTTCAAGGTAGTTTTAAACCAGCTCATTAACAATGCAAACGATTATTTGGGTTTTAAACTCGAAAAAGTAATTGTGGTTTTAGCGGAGTTAGTTGATGATCTTGAAATTAACCGCTTTAAAACAGATGTCTTTTTCACTGGTTATGACTTTAACCACCAGGACATGTTGAGCAGTGAAAAGAACCGCTTCTTGCTCAAAAACCGTCATATCAACCCCAATGAAGTTTTGGACTTAGTGGCTTTAAACTACCGTGATTTAACCACTGGTAAAATTAGCAAAAACTTTAAGTACAACTGCAGTTATCGGGCAAATGTAATTTATTACACCACTAAAAACTCACTAGTCAAGGAGCTCAAACCGTTTTTAAAGCGCAATATTATTGTCAAAATTGACAAAATTGTCACCCACCACATGGTGTTAGCCCACTCAATTAAACAAATCAACAAAAACAACCTCTTTGTGTATTTAGGGGAACACACTACTGACCTCATGTTGTTTATGGACAATGCCTTAGTTGATTTAGTAAGTGAACCCTTTGGTCGGGTTAACTTCTTGGAATCTGACAATGACAGTGAAAACAAAGCGCTGTTGGAATTCCTAGTGGACTCTACTGCACGCATTGGTGACTCGGGCTCGATTGGCATGGCTTACACCGATGGTTCTACTTATAAGGAAATTAGAGCTGTTACGATTGATGACCTCATTCAAAGTGTTAATGAAAAAACGAAGTACTTAATTGATTTCATTAACACTAACACGGAAATCTTCTTCCAAAAGTACGGCTTTTTACCCAGCACTTTACTGTTTTACACTAAGAGTAAACAGTTGCTTAACAACTTTCAACTCAACCAAAACCTGTTGAGTAACCACTTCAAAACTGTAACCATCTTTAAAAACGAAGTTCAGTTTGTGAGTCGCAAATACTTATTGAACTGTCAAGCAATTGCGCTTTCTTGAAGACAAGACCAAATTAATGTTAATCCCACTAGCTTTGCCCTACCGTTTTACTCAGACAAAATTGAAAAGCAGTTTAAAAAGTCGTTTTTAATGTTGAAGGTACACACGCAAATTAATAAGTTAGTGCAAAAACTAATAAAGTAAGCTTTATGGATTGAATACAAACAGCAGGTGCTGGTACTCAACTACCAGAGAACAACATTAAGATAGCCGTTTTTGGAATTGGTGGTGCTGGTAACAACATTATTGATGACATGTTGCGCATGCACCCCGAATTGCAAACGGCTAACGTCCAGTTCTTTGCTTTAAACACTGATTTACAGCACTTAAAAACAAAGCGCTATGTCCAAAATAAGGCTGTGATTCAGTTTGAAGAATCCAAGGGCTTAGGTGTTGGTGGTGATCCCCAAAAAGGTGCCGTTTTAGCACACCATTTTTTAGAGCAATTTCACAAACTTAGTGACAGCTTTGATTTCTGTATTTTAGTCGCTGGTTTTGGTAAGGGGACTGGTACTGGTGCTACCCCGGTTTTTAGTAAGTTTCTTAGCAACAAAGGGGTGTTAAATCTCTCGATTGTCAGTTACCCCGCAATGTGTGAAGGGTTAAAGGCACGCGAAAAAGCAGCTAAAGGTTTAGAACGCTTAAACCAAGCGACTGACAGTTTTATGCTGTTCCGCAATGATCGCTGTACTGATGGCATTTACCAACTAGCAAACGTTGCCATTGTCAAAACAATTAAAAACATTATTGAACTAATTAATCTACCACTACAGCAAAACATTGATTTTGAAGACATTCGTTCTTTCTTTAAAAAACCAGCACAACGTTTAGAGAATGAAGCTAACTTGTTTCGCGTAACTAATACTTTTACTTTTAGCTTTGACGCGCACAATACAATTGAGCACTTTAGCCACAAATTGAAAAACTTTGAGTATGAAGGTTTTTTTGACCATAAGGTAGAAGGGGCGCAAAAGGTAATTTTAAAGGTGTTAGTCAACCAAGGTTTATATCCGCTTGATTTAACCCAAATTCAAGAGATTATTTGAGCCAAAATTGACAATCATAACCTAGAGGTACAACTTGGAGTTGACTTTACTGATGCCAACCCTTCAGTGCAACTGTTTTTCTTAATGGAGAAAAAGCAAGCAGTGTCCTCTGACTTTATTCAAAAACCAGCATTTATCTCAGTTAAAGAAGTAAATCAGAAACCGGCCAAACCGTTCCAAGTTTTGAATGATTTAAAGGAGCTGGGACTGAAATACGTCAAACAGCAAACCGGATTTAATTATTAGTGGTCTTTCTTCTTTAGAATAAGCTAACTAATAAGAAGGTTAATGGCTGTTGCAAGTAAGAAGTTCACTGAACGTCAATTTTTATTTTTTGTTGTCAACTATATTGCTGGCTTTGGTTTTATTTCCACTGCCCTATCACTGTTTGAGCTAGGACCACTTTCATGAGTGATCTTTTTTCTCGTTAGCTTAATTAGCCTCATTGTTACCTTAGCATTTGCCAGGGTATCAGCGATTGACAAAAATAATTACGGCGGCGCTTACCTGTGAGCCAAAAAAGCCTTTAACAGTGAACGGTATTCCCACCGCTTGTTTATCTTCTTTACCGGGTGAAACAATTTTATTGCTTCACCGCTATACGCCGCTATTGCCCCGCTTTTTATTCTCTCTGCTTTTAAAGGTATTGATGGTATTCAGGGCAATGCAGCTAACACTTGAATCCTTATTGCCGCTGGTTTTACCTTTTATTTACTGTTAGCCTTTCTTTCCACAAAGGGAACTTCCCTCAATAAAAAGTTAATTGCGGTATTTGCCTCAATTAAATGGGCGGTGCTTTTGAGCACCTTAATAGTAGCACTTATAGTTATCGGCAAAAATGGTAATGGTTATGCCATTAACAACGACATAAAACAAGGCCCTTTTAAAGAACGTGAAATTACCTTGGGTGCAGTTGCTACTGTCTTTATTACCTTCTTTTATTCCTACAGTGGTGCGGAGGACATTTCAACAATGACACCGGATGTGAAAACCACTAACTTCCGCAAGATTTTAATCATCTCTTTTATTGCGGTATTCTTGTTTTACTTTGTGGGCATTTTGATCTTTAATGGTTTATTGAATGTTTCACAAAGCGATGATGATAAGGGGAAAGATGGAAGAATAGCTAGTGTAGCTGATATTTTTAGATTAGCTGGCGGTTTAGGGGCACTCATTTTTTACGGTGTGGGTGCTTTATTCAATAACGTATCTACCAGACTTTCGACCATTATTGCTAACTCACGCAAGATCTTACCGTTAGCACAAGATAACTATTTACCCACTACCTTTAGCAAAGCTAACAGTAAGGGCGAGTTCCAAAATGCAATTTGGTTTACCTTTGGTACTACCATCATTACGATGACGGTACTGTTCTTTGTTCCGCTAATAACCAGCAACTTTGACTTCGACAAATCAACCGAGTATGCCGCCTCGATAGGTTCGGCCGCTACACTGGTACAATATATCCTTGTATTTTTTATAATTTTTAGGCTTATTTATAAAAAAGAGCAACTTTATCAAAAAAACTGGACACGCGAGTTAGAACAAATACTGTTTGGGATTGGTGTCTTTATTATTCTTCTCATGTTGCTCTTTTATCTCTTTCCAGTAATTGATGGAAAAACTAAATGGCAATTGAAAAATACCCTCACTTTAGTGGTGTATGGGGTGTTGATTTTAATTGGATTTGCCCTCTTTATGACCCAAGAATATAAGCGCAATAAACAAAAGGAAAGCTATGTCACACCAAGAGCAGTTACATAAACCTAACCGGCAACAGTTTAGCGAAAAGCAATTTATTGCTTTTGCTTTTAACTATGTTGCTGGGTTCGGCTTTATTTCCGTGGTGCTAACCATGTTTAAGCTTGGCCCGTTTTCTTACTTGATCCTAGGGCTAGCAGCTTTAGGTATTTTGGGGGTAATGTTGTCCTTTTCGCGCCTTTCAATTATTTGTGGGAGCAAGGCTTACGGTGGTAGTTATTTAATAGCCAAAAAAGCACTTGGTGCGAAAACAATCACGGCGCGTTTCTTTACTTTTTTGAGTGGTTGAAATGTTTCCTTAACCGGTCCTTTTAATGGGTTAATTGTGCCAGCGGTGCTAGTTCTCTCGTTTGCTGATATTAAAGCAGTGAAGGATAACAATGGTGCTTTAATTGGCTTACTGGTCGGGGGCTTTGTTTTATTTGGGGCATTAAACTTCATCTCCCTGTTTGGCCTTAAAATGAATAAGAACGCCATTCTCTTCTTTGCCATAGTGAAGTGAGTAGTTGTTCTGGGCGGTTTAATTTTGGGAATCTACCTCATCGGTACGAACCACGGCCATGGTTTTGTCGAAAACAACACTTTAGGTGAACACATTGAAGATCTCAGTTTCCTTAAAGTTATTTCTACTACTGTGGGGATGTTGGTGGCCTTTGCTGGTACCGAAGATCTTACTGCGATTACCCCGGATGTGAAGTCAAAAAACATCCGTAAGTGTTTCTTATTGATGTTTGGTGCAGTTACCCTGCTTTATCTAATCGGTTTTGTCATTATTAGTGGGATTTCTGGTTTAAATGGTTATGGCCTTGATGGTAAAGAAAAGAATGAAAAAGCGATTAACACCTTTGGTTCGATTTACTTTCAAGCAGGCGGTAAGTATTTAGGCATCCCACTGTTGGTCATCTTTGGTCTGGGGTTTTTACTCAATTCCTTGGCATCACGATTAGGCATGACCATTACGACAGCGCGCAAATACGTTGCGTTAGCCCAAGATGGTTTCTTGCCGTCCTTTATTAACGAACAAAACAAGCACCACGAGTACCATAAGGCAGTGTGAGCTAGCAACATTATGACCTTAGCAGTGATGGTGCTGATGATCATTGTCCCCTTTTTACCTAATGATGAAAACCCCGGTAAACAGCTCGTTATGTTCGATGCCATTAGTGTTTTAGTTGAAGTGGCGATTGAACTAGCAGTGTTAATTTCCTTAATCCAGTACTTTATTACCTACATCTTCTTCTTTATGATTCTGGCCAAAAAAGAGGGTAGTGCCAGTGTTTCGTGATGGGAAATTGCCAGTTATGGCGTAAGCTTTGCCATTATTACGGTTTTGTTGTTTGTTAACCTCTTTCCTATAACGGCTTGAAAGAACACCAACACCTTTAAGCTAAGTATCCTAGCGGCCTTCTTTGCCTTGGGTATTGGCTTCTTTATCCACTCGGAAATAAAACACAAAGGACAATTAGTCAAGTCAGTCGAGTGTAATTAAGATCACTCCATTAAACCGGTAGAAAAAGCCGTCACGCATTTCGGGTGGCGGTTTTTTCTTGTTCTTTTGACTGCCATAAGACAAAAAATACAAAAAGCATTCAGCGTTTATCTCTTAACCACCGGTTTGAATAGTTAATTGAGCTGCTAAAACGGCTTTTTAGTGTCTTATAAGCGCTTAGAAAGCCTTTTCTGTGTCATTTTCCTAGCTTGAGCCTAAAATGAGCCCTACAGGCCGTTTTGGGGGCTTACAAGCGGTTATTAACATTGTTTTAACAGGTTATTAACATTGTTTTAAAAGGCTCCATTAGCTCATTTTTGCAGTGTTAAAACCGCTTTAAAGCTTATAAAGCTTTAAAAAAAGGCGTTTAAATGAGCTTTAAAATAACCGTTTTAAGTTATTGCAAAAACTCTTTTAAAAAAGTTATTCGCTTATTTTGCAAGCTTAAAAAAAGATTTTTATACAATGCTACACCATCGAAGTTTAGGAGAGTTATGCAGCAGTATCTAGATTTAGCCCGTTATGTTTTAGAGCACGGAAAATACCGTCCAAATCGCACTGATACTGCTGGCATTGGTGTCTTTGGTTACCAGATGCACTTTGATATTTCAAAACACTTTCCGCTCTTGACTACGAAGAAGGTGCACTGAAAGTCGATTGTGCACGAACTTTTGTGGTTCATTAAGGGTGACACCAATATTAAGTACCTAGTCGACAATAAGGTCAACATTTGGAATGAATGACCTTACGAAAGCTTTAAAAAGTCACCTCACTTTAACGGTGAGAGTCAAAAGGAGTTCATTGAACGGATTCGACAAGACGCGAAGTTTGCCCAACAGTTTGGTAACCTCGGACCCGTTTATGGAAAGCAATGGCGTGACTTTAACGGAGTCGATCAATTGAAAAAGGTAATTGCGCAAATTAAGGTAAATCCGTTTTCGCGGCGCTTAATTGTGTCTAGTTGAAATCCCAATGAGGTGGATCAAATGTTGCTCCCGCCTTGTCATTCACTGTACCAGTTTTACGTGCAGGACGGGCAGTTGAGCTGCCAGTTGTACCAGCGCAGTGGTGATATTTTTCTAGGTATCCCCTTCAACATTGCGTCCTACTCTCTTCTGGTGTACTTAGTTGCTAAGGAAACTAACCTAAAACCAGGGAGCTTTGTCCACACCATTGGCGATGCCCACATTTACGAAAATCACTTGGAACAAATTAAGTTGCAATTAACACGTCAACCCAAACCATTGCCCAAGGTAGTTTTAAAGAGTGATAAATCAATTTTTGATTATCAATTCGATGATATTGAACTGGTGGATTACGACCACCACCCCACAATTAAAGGAGAGGTAGCAGTTTAAGATGGTAAAAGCAATTTGAGCAATGGATCAAAATGGGTTAATTGGTAACGGTAACAGTTTGCCCTGAAGGATTAAAGCGGAGTTACAGCACTTTCGCCAGACCACGTTGCACCAAGATGTATTAATGGGCTCGGCTACCTATCTTTCCTTACCGCCGGTGTTTAGTGAGCGTAACGTTTATATCCTCACCCGTAACCTCAACTTTAACCCGCCTGATAAGGGCTGTTTGACTAAAGTAATCCATGAATATGAAAACTTTATCCAACCTTATTTGCACCATCCCGATAAACACCTCTACATCTGTGGTGGGGCACAAGTTTATGAACAGCTCATTCCCCGGTGTGATGCGTTAATTGTCTCTACCATTTTCGGTAAATACACCGGGGACAAATACCTCAAAGTGGATTTTAGTCCCTTTGAATTGACCAAAGAGATCAGTTTTGCTGAGTTTAAGGTCGCTTACTATCACAAAATTGCACGATAAAACAATATGGCTAACACAAAAAAATACTTTTTAGAATCAGTTTCTCCCTTAGAATATGCACAAAAAAAGTTTCAGGGCAACTTGCGCTCGGTTAACTGAAACCTAGTGGATGATGAGAAGGATCTGGAAGTGTGAAACCGGATCACGCAAAACTTCTGGTTACCAGAAAAGATTCCGGTATCCAATGACATTCCATCATGAAAGCAGCTGTCTAAGGAATGGCAGGATTTAATTACCAAAACCTTTACGGGGTTAACATTACTTGACACCATCCAAGCTACCATTGGTGACATTAAGCAAATTGACTATGCTTTAACAGATCACGAGCAGGTAATTTACGCCAACTTTGCCTTTATGGTGGGGGTACACGCGCGCTCTTATGGCACCATCTTTTCCACTCTGTGTACTTCAGAACAAATTACTGAAGCACATGAATGGGTGGTGAAAACCGAAAGCTTGCAAAAGCGCGCTAAGGCTTTAATTCCCTATTACACCGGGAAAGACCCATTAAAGTCTAAGGTAGCAGCCGCTTTAATGCCCGGGTTCTTACTGTATGGTGGGTTTTATTTACCGTTTTACCTCTCCTCGCGTAAACAGCTACCCAACACTTCGGATATTATTCGCTTAATTTTGCGCGATAAGGTAATCCATAACTATTACAGTGGGTATAAGTTCCAGCGTAAAGTGGAAAAGATGTCCAAGGAAAAACAGGCTGAGATGAAACGGTTTGTCTTTGATTTAATGTACGAACTGATTGAGTTGGAAAAGGCATACCTCAAGGAACTGTATGAAGGGTTTGGGATTGTAGAAGATGCCATTAAATTCAGTATTTATAACGCTGGTAAGTTTTTGCAAAACTTAGGGTATGACTCACCGTTTACAGAAGAAGAAACGCGGATTAAACCAGAAATTTTTGCCCAATTGTCAGCCAGAGCCGATGAAAACCACGATTTCTTTTCTGGTAATGGTTCGTCATATGTGATGGGAATTAGTGAAGAGACCGAAGATAAGGATTGAGACTTTTAACAATGCATAAAGATATCAAAATAGTCGACGCTAGTGCGATCGTTAAACCAACTGGAACACCGTATGTAGTGTATTTTTCCTCCATTTCTAATAACACCCACCGTTTTATTGAAAAATTGGGGTTTGAACACACCCGCATTCCGGTTAATTTAGATGAACAGATTGAAGTAAACCAGGAGTATGTGCTCTTTTGCCCCACTTACAGTGGTGGTGGGGAATACACATCGGGGGCGGTACCCAAACAGGTAATCCACTTTTTAAACAACAAGCACAACCGTGATTTGTGTCGTGGCGTAATTTCGTCCGGGAACACTAATTTCGGCAATACTTTTGCCATTGCCGGGCCGATTTTGTCGAAAAAGTTAAACGTACCACTTTTATACCAGTTTGAACTTTTAGGCACGAAAAACGATGTAGAACAAGTTCAAACCATTATTACCAATTTCTTTGGTAAAGCCAAATAACCGTTATTAATGATTAGCCAATTATGTCAGTTAAAGAAAAGATTCCTGCTTTCAACACCCAAGAAGACTTAGAAAGCTATATTTCCTTAAATGCCTACACTAAGGTGTACGGTGATTTTAAGATGGATTTACACGCTGTGGAGGCTTACATCCAAGAGCATGTCAAACCCAAAACAAAGGTGTTTCACTCCACCAAGGAACGCTTAGACTTTCTAGTAAAGAATGATTATTACGATGAGAACATCATCAACATGTACAGCTTTGAGCAGTTTGAGGAAATTACACGTAAGGCTTACGCTTACCGTTTTCGTTATGCTAACTTTATGGGGGCATTTAAGTTTTACAACGCTTATGCACTGAAAACCTTTGATGGGAAGTGGTATTTAGAAAACTATGAAGACCGCGTTGTCATGAACGTGCTCTTTTTAGCCAATGGTAACTATAACAAGGCTTTAAAGTTACTCAAACAAATTATTACCAACCGCTTTCAACCAGCTACCCCAACTTTTTTAAATGCGGGACGAAAAAAACGTGGTGAGTTTGTGTCGTGTTACTTACTGCGCATAGAAGACAACATGGAATCAATTGGTCGTGCCATTACCACTACCCTACAGCTCTCCAAGCGTGATGGTGGGGTGGCGTTATTGCTCACTAACATCCGCGAATCGGGTGCGCCAATTAAAAAAATAGAAAACCAGTCATCAGGCATTATTCCAATTATGAAGCTGTTGGAAGACTCTTTTTCTTACGCCAACCAATTAGGGCAAAGACAGGGAGCAGGAGCAGTGTATCTCCATGCCCACCACCCCGATGTCATGCAGTTTTTAGACACAAAGCGCGAAAACGCTGATGAAAAGATCCGGATTAAGTCGCTGTCTTTGGGTCTAGTTATTCCCGACATTACCTTTACTTTAGCTAAAAACAATGAGGAGATGGCCTTGTTTAGTCCGTATGATGTGTATGAGGAGTATGGCAAACCCTTGTCAGACATTTCAGTCACGGAAATGTATTACGAACTGTTAGCGAACCAACGCATTAAAAAGACCTTTATTAATGCGCGCAAGTTCTTCCAAACAGTGGCGGAACTGCACTTTGAAAGCGGTTATCCTTACATCTTATTTGACGATACGGTTAACCGGCGCAACGCGCACCCCAACCGCATTGTCATGTCTAACCTCTGTAGTGAAATTGTCCAACCCTCCACTCCATCGGAGTTCCACCACGATTTGGCTTTTAAAAAGGTTGGTAATGACATTAGCTGTAACTTGGGTAGCTTAAACATTGCCAAAGCAATGGAAAGCGGACCGGAGTTCTCGGAGTTGGTTAAACTAGCGATCGAATCACTTGATTTAGTTTCAAGGGTAAGCAATTTAGAAACGGCTCCATCGATCCAAAAGGGTAATTCCGAAAATCATGCTTTGGGATTAGGGGCAATGAACTTGCACGGTTTTTTAGCAACTAACCAAATTTATTACAACTCCCCTGAAGCAATCGACTTTACTAACATCTTTTTCTATACGGTAGCTTACCATGCCTTTAAAGCATCCAGTGAGCTAGCTTTAGAGAAGGGTAAGTTTAAAAACTTTGAAAACACCAAGTTTGCTGATGGGAGCTACTTTGATAAATATATCAAAGTAGAACCAGACTTTTGAACACCCAAAACCGAGCGCGTTAAGGCGTTGTTTCAAAAGTACCAAGTGGAAATACCAACACGTGAAAACTGAAAGGAGTTAGCACTCAACATTCAGAAAAACGGTTTGGCCAATTCCCACTTACTGGCAATTGCTCCAACGGGTTCAATTTCCTATCTCTCCTCCTGTACTCCTTCACTCCAACCGGTAGTATCCCCGGTAGAAGTGCGCAAGGAAGGTCGGTTGGGACGGATTTATGTACCCGCTTACCAACTCAATAAAGACAGTTACCCTTTTTATAAAGATGGTGCTTACGAACTGGGTCCCGAACCAATTATTAACATAGCAGCAGCGGCACAACAACACGTCGATCAAGCGATTTCGCTCACCCTATTTATGACTGATAAGGCTACCACTCGTGACCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTTCTTCCATTTACTATGTCCGAGTGCGCCAGGAAGTACTAGAGGACAGTGAGGATCACACCATTCAAATGCAGCAGTGTGAGGCCTGTGTGATTTAATAATACATATAAATACATATAGTAAGTCTAGCTTTAAGCATGACTTTAGCAGGGGTTAATTTGTTATAATTTCACGCTTATTTGCGTCTTCTGCGGTTCCTAAATTTTGTTTATACAAAATACCAAAAGAAACGGTTTTATTGAAAATGCATGCTATTGTAGTCTGTGGATCAAAGCAATATTTAGTCCACGAAAATGACACTTTTTTTGTTGAAAAACTTGAAGCGCCAGTTGGTAAAGAAATCCAACTCGACAAGGTTTTAATGTTGGATGAAAAGATTGGTGCGCCATACTTGGAAAAAGCGCGAGTAGTGTGTGTGGTGGAAAAGCACGGTTTACAACGCAAAGTAAACGTGATTAAACACATCTCACAAAAACACCACTTAAAAAAGTATGGTCACCGTCAACCTTACACTAAGTTAAAGGTAGTGCGCTTTGTCCATGATTAAACTAACTGTGTCGCACCACAAACTCACCGCTTCCGGGCACGCTTTATTTGCCAAAAAGGGGCAAGACATTGTGTGCGCTGCTGTTAGTGGTATTATCTTTGGCGCTTTGCCCTGGTTTGAAACCAACAGCATTGCCGTTCAAGAAGATGCTACTGTACCCAGTTTAAGCTTGGAGTTAGTGCAACCAACCGCTAAGCTTATCACAGGATTGAGTGTGGTGATTATGCAACTTAAAACCCTCGCGCATTCTTACCCGCAGTTTATTTCGTTTGAAGATCAACGTAAAGATGAATAACAAATATTTTTTAACCAAAATTGACTTACAGTTTTTTGCTTCCAAAAAGGGAGTTGGTTCAACCAAGAACGGCCGTGATTCTCATGCTAAGCGTTTAGGTGCTAAGAAGGCTGATGGTCAGATGATCCGTACTGGTCAAATTATTTACCGCCAACGCGGTACTAGGGTATGTCCCGGTGTAAATGTTGGCCTTGGTAGTGATGACACCTTGTTTGCTTTAAGCGACGGCTTGGTAAAATACCAAAAGTTTGGACCTAAACAGGGCAAGACCCGCGTGAGTGTGGTCAAACACAAACTAGATGCCTAAACTCCTAGGAAGCTTTATTTCGTTTAAATCACCCCATTATCTAGTGGGGAGTGTTCGTGACGCTGTTAGCATTAAAGCACAAGCGTTCATGATTTTTTTGGGCGCACCACACACGGCACTACGAGTCGATCCTAATCGTATGCAAATTGACGAGGGTCACACATTGATGGAACAACATAACCTCAGTAAGTCTGGGATGGTTGTCCATGCTCCTTACATTATTAACTGTGCCTCCAAAGATCCGGTAAAGCAAACCTTTGCGATTGACGTTCTGACAAGGGAGGTTAAACTGTGTCATGCCGTGGGTGCTAAACTAATCGTTCTCCACCCCGGTTCAGCGGTTGAGCAAACTCAAACCCAAGCGTTAGATCACCTAATCAAGGTCTTAAATACAGTGATTGCCAACACTAAAGAAGTCATTATTTGTCTCGAAACGATGGCTGGTAAAGGTAATGAAATTGGCCGTGATTTAGACCAGTTAAAGTATGTGATTAACCACATTGAACAACAAGAGCGGATTGGTGTTTGTCTAGATACCTGTCACTTCCATGACAGTGGTAATGACTTTAACAATACTGCCGAAATTATGGAAACGATTGACACTAAACTAGGCTTTGAGTTTTTAAAGGTCATCCACTTAAACGAGTCCAAAAACGTTTGTGGTTCCAAAAAAGACCGTCATGCCAATCTAGGTGAGGGGATGATTGGGTTTGATAATTTAATGCGCTTTATTGCCCAACCGCAAATAAAGCAAATACCGATTGTTTTAGAAACCCCATCTGACAAGCATAATTATCCCGCTGTATATGGTGCTGAAATCGAAAGGATCCGTGCTTGATTTGGAGCACGATAACCAAACACTAAAGGATTACATTAGTGTGTTTGAACGCAATAAGATGCGTTTAACCCAATCACGCTTAATGTTGCTCCAATGCTTAGTGCAACACCGTGACTGGCACACCTTAGCTGAGCTCAAACACCACTTGGAACAAAACCAACAGCGCACTACCTTAGCTTCTATCTATAACAACCTCAAGATTTTTGCTAAGTTAAAGCTAATTAATATTTTTGTAGATACCAATCGCTTTGAAACGTATTATTGCTTAAGACACGAAAACCACAAGCACATTTACTTCTTTGACGAAAACCAGCGCAAGTTTTTAACCCTACCACTACAAGACAAGGAAGCGTTGAGTTTAATTGGACATAAAAGTAAACATGGCAAAATTAAACTGAATGACTTTTACATAGTCGCTAGCGGTACACTTGAAGATGATCAATAAGCCAAATCAGTTTGTCAACCACTTAAGTGCTTTAAAAAAACACTTTGCCTCTTATAAGGAGCTCCGTGAAGCGTTTAACGATTATCACAAGCATAACGGTGATGAGTTGACCACCTTCTTTTTGCACCAGTTTGATAAGGTGATGGAACTAGTCAAGCAGAAGGACTTTAAAACTGCCCAGTCTCGCTGTGAGGAGGAATTAGCCGCCCCTTATCTACCAAAACCTTTAGTAAGCTTTTTCCAATCGCTACTCCAGCTCGTGAACCATGATTTATTGGAACAGCAAAACGCGGCCTTGGCTAGTTTACCCGCAGCCAAAATCATAGAGCTTGTTTTACAGGATTACCCCAATAAACTCAACATGATTCACTACCTTTTGCCCAAAACGAAGGCGTTTGTCAAACCACATTTGTTACAACGCTTACAGTTTGTGTTAACTGATTCGGAGTTGTTGGAATTAAAGCGCTTTTCCTTCTTTCAAGCACTCAACCAGATCCCGGGCTTTCAGGGTGAGCAAGTTGAGTACTTTAACAGTAAGCTCAAACAAAAGTTTACCTTGACCTTAGGGGAGTTTGAAATTGCCCAACAGCCAGATGCCAAAGCATACTTTGAGCAGTTAATTACCCAAATCCAACAGCTCTTTTTAAAAGAGCCGGTTAACGCTGAGTTTGCCAATGAGATTATTGATGCCTTTTTGGTGAGCTATTTCCCCCTCCATCCCCCAGTACCTTTGGCACAATTAGCTGCCAAGATTTATGAGTATGTCAGCCAAATTGTGCTTAACGAAGCGGTTAACCTAAAGGATGAACTGATCAAACTAATCGTTCATACGCTTTACGAACAGTTAGATCGTCCTGTTGGGGATGAAAATTAAAATATAGCTCAGATCTAGTTTAGAATGAAACAATACAAGCTTGTCAATACAACCCAAAAAGAAAAGACGCTTTGTCTTGAAATAGCAATTGACACGAAGCTGTGAAAGGAGACACAGCAAAAGCAAACCCAAGATTTAACCAAAAACATGAAAATCAAGGGTTTTCGTAAAGGTAAAGTGCCTCCAACTTTAGCTAAGGATTACTTAGACCGTGCCGAGTTGTTGCAACGTAGCGCCCAAGCGGTAATTGATGCCATCTTCCAGCCGTTACAACAAGAAGCAGTAATTGCTGACAACGAGAATGTAATTGAGGACTTTCCTACCATTGACTTTAAAACAATCAATGAGAACGATTGTGTTATTCTGTTTGACTTTGATCTCGTACCCCAGTTTGAACTACCTGATTACAAGCACATCAAGGATTTAAGCCCGATTGTACCCCTAAAGGATGAGGAGTTCAACAAGGAGTTGCACAACATCGAAAAGAACAAAGGGAAACTAGTTGATGTTAGTGACAAAGCATTGGCCAACAATGATATTGCCGTCATTGACTTTGTTGGCAAGGTAGATGGTAAGGTGCTTGAAAGCGCTACTGCCAAACAATACGAATTAACGATTGGCTCCAACAGCTTTATTGATGGCTTTGAAAGTGGTTTAATTGGCATGAAAGTAGGGGACAAGCGTCAGTTAAAGTTAAAGTTCCCCAAGGACTACCATGCTGAAGAATTGAAGGGTAAACCAGTAGAGTTTGACATAGAGCTCAAAGCAATTAAGCAGTTAGAGATCACCCCCATGGATGAAACTAACTTTAAGGAGTATTTACCCGCACAATACCAGGGTTTCAACTCACTCAAGGAATTTAAAACTTACTTTCATAAGTTAGTTTCCGCTAAGAAACTGGAAATTACCCTACAAGAAAACAGTGTTAAAATTCGCCAGTTCTTTTTAGCTAACACCACCTTACCTTACATTCCTGATTCCTTAATTAAATTGGAATCCGATCGCTTACTGAGAGCGCAAAAGGATCAAGCGGAGCAGTACAAGATTCCCTTTGAGAGATTGTTAGCAGCTTCCAAGCTTTCATTGGAGCAGTTGCAACAACGTAACATAAAAGAAGCACGTGATAACGTTACCTTTGCTTTAGTGATGAAACGAATCGCTGATGTGGAAAAAATCAAGGTTGATAACAAAAAGATTCACAGCGAAATCGAATCGATTATTGATGTTGAATACCCCTTTGTTAATGCCGAGTTGAAAAAACAAATGTTCCACAACATGGAACAACAAAAGGACTTTGTGGAATCGATAATTTTGAACCGTTTAACCACTACCAAGATAATTGAGTACTCCACTCATTAGCACTCAAAGTATTAGAGTGCTAAAAAATGTGTTACAATTTAGCATTCTTCAACCTCTTATCTTAATAACTATGCCAGCTGTAAAAAAACCACAAATTCTCGTTGTGCGTAACCAAGTTATCTTTCCGTACAACGGCTTTGAACTTGATGTCGGTCGTGAACGCTCGAAAAAACTAATTAAAGCACTCAAAAACCTCAAAACCAAACGTTTGGTGTTAGTCACCCAAAAGAATTCAGATCAGCTCAACCCTGAGTTTGATGACATTTACCATTGCGGTACTTTATGTGACATTGATGAAATTATTGAAGTACCGAGTGAAGATGGCAAGACAGCTGACTATAAGATTAAGGGTAAAGGATTACAGCGCGTTGCCATTACAAGCTTCTCTGATGCTGATTTAACCAAGTACGACCATCACTTTCTCAACTCTACCCTAACAGAAAATAAAGCGTTAGATAAGTTGTTGGAACGGATCTTTCCCGATAAAGAAGACTTTGCTGAAATTTTAGACAGCTTAAACAGCTTCTTGGAACTGCAAGAGTTAAAGAAGCTATCTAAGGTACCAAAAGACATAAAACGGTATGACATTATTACCTTCAAGCTTGCTAGCTTAATTTTCAAAGACATTACCCTCCAACAAGCCATTTTGGAAGAAAATGACATTGAAAAACGGTTACAGAAGATCATTGGTTCTGGCATCGAGGATTTAGGTCACATCAGCGAAGAAGCCCGCGCTAAACAACGCGAAAGTGAGTTTGACAAGATTGACAACCGCATCACGCGCAAAGTTAACGAACAGTTATCGCGCCAACAACGTGACTTTTACCTCCGGGAAAAACTCCGGGTTATCCGTGAAGAGATCGGGATGACTTCCAAGAAGGAAGATGAAGTATCCAACATTCGCAAAAAGCTAGAGGAAAACCCATATCCAGAGCACATTAAAAAACGGATTTTAAGTGAGTTAGATCACTTTGAAAACTCCTCCTCTTCATCACAAGAATCAACCTTAACCAAGACTTACATCGACACTTTGATGAACTTGCCATGGTGACAGGAAAGCAAGGACAATGCTGATGTAAAAAACCTGATTAAGATCTTGAACAAGAACCACTCAGGACTAGATAAGGTAAAGGAACGAGTGGTTGAGTATTTAGCAGTACAGCTCCGTACCAAAAAGCTTAAGGGTCCGATTATGTGTTTAGTCGGTCCACCTGGAGTTGGTAAATCCAGTTTAGCCAAATCAATTGCTGAAGCGTTAAACAAGCGCTTTGTTAAGGTTTCATTGGGCGGGGTGCATGATGAATCGGAAATCCGTGGTCACCGTAAAACCTATTTGGGTTCGATGCCAGGACGGATTTTAAAGGGCATGGCACGTGCTAAGGTTATTAACCCACTGTTCTTGTTAGATGAAATTGACAAGATGACATCCTCCAATCAAGGTTACCCTTCCGGCGCTTTGTTAGAGGTATTAGATCCGGAGTTAAACAATAAGTTTAGCGATAACTATGTTGAGGAGGATTATGACCTGTCTAAGGTAATGTTTGTAGCCACCGCTAACTATATCGAAGATATTCCGGAAGCCTTGTTAGACCGGATGGAAGTGATTGAACTTACCTCTTACACCGAACAGGAAAAGCTACAAATTACAAAGAGCCACTTAGTTAAGCGTTGTTTAGATGACGCTGAGATTAAAACAGATGACCTCAAGTTTACCGATGAGGGTATTAGCTATATCATTAAGTTTTACACGAGGGAAGCGGGGGTGAGACAGTTAGAACGCTTAATCCAGCAAATTGTGCGTAAGTACATTGTTAACTTGCAAAAAACCGGCGAACAACAGGTAGTAGTTGATGTAGATTTGGTAAAGAAATACCTCAAGAAAGAAATCTTTGACTATACCGTGCGCGATGAAGATGCACTTCCGGGAATTGTTAACGGCATGGCTTACACCCCAACTGGTGGTGATCTGTTACCAATTGAAGTAACCCATGTAGCTGGTAAGGGTGATTTAATTCTGACCGGCAATTTAAAGCAAACAATGCGTGAAAGTGCTAGCGTAGCGTTAGGTTATGTCAAGGCCAATGCGCAGAGCTTTAACATTAACCCTAACCTCTTTAAAAAGGTTGACATTAACATCCATGTTCCAGGTGGTGGTATTCCCAAGGATGGCCCGAGTGCTGGCGCGGCTTTAGTTACTGCCATTATCTCGTCTTTAACTGGCAAAAAGGTTGACCCTAAGATTGCCATGACCGGGGAGATTACCCTAAGGGGTAAAGTAATGACCATAGGCGGGGTGAAAGAAAAAACCATTTCCGCCTACCGCGGTGGAGTACGCACCATCTTTATGCCCGAAAAGAATGAGCGTTACTTAGATGAAGTGCCTAAAGACATCGTTAAGGACTTGGAGATTATCCTAGTAAAGGAATACAAGGACATCTACAACAAGATATTTAATTAATTTTTACCAATTCAGGACTTCAAGGTGCCTAAGATCAGATAATTACCCTTTTTAAGGGTAAAATGGTCATCATTAAACTAATTCATTTCTTATAAGTGTTAGCTAGAAGAGGACGACGGCAAAAGAGTGCTGTAGGACTTACTTATTTTGCTTACCTTGCACAGCAAACACTCCGCAGCAAAACCTGCTGGGTGATGTTTGTGCTGTTGATGCTGGCGTTAGTGATGCTGTTCATTGTTCAATCGAGCACCAACGTGATTGCGCTTTGAACAAACAATGTCTTTCAGTTTTCACTCATCTTAATACCGTTAGTGACACTGTTAGCGGGCAGTATTACCGCTGACATCTTTATTGAAGGGTACAACAACGGGATTGAGCTGTTCTTGTTCTCTAAACCTATTAGCCGTGACCGCATTTTGCTCATTAAGTTACTAGTGCTGTTAATGTACTTGAGTATTGTAGCCGTACTATCCACTGCTGTAGTGAGCTTGGGTTACTTTACGCGCAATGAGATTGAGAGTTATTTAGATGATTTAGCTTTAAGTATCTTTGTCGGTACCATTTTAAACGGCATTATCTTTAGCGCTGTAACTATTATGTTAGCCACGCGCTTTAGCAACATCCAGAGCTTAATCATTTTGCTCTTGTTTGTGTCGTTGTTTAGCTTTTCCTCACCAATTGCGCGGTTAATCTTCGACACGCCTGCTAAAATGTTGAGCCAACAAGGTTACAGCGTTCGCCAAATTAACGGAATTGCGCACAGCGAAAGCCCTAATGTAGAGCGTAAAATTTACGTTTCGGTCAAAAAGCGGCGTTTAACTGGGGTGGATCGCGAAGAAAAAGAGCTTTGAAAGAGTGATGAGCAAGCAGCACTGCGTGGTAGAAATTTGTATGAGGAGGCACAACGAACCTCCTCTTACATTAATTCCTTTTACACCAACATTGGTTACTTATTTGGCTCGCTTTACCGCTTGGGTGCGTTAGCTGATGAAAATAACTTTGATGCATCAGTAGCAACCCAAAACACCAAAATTCGCTTTGGTAAGTTAGTTGATTTAGATAAGAGTACTGACATTATTGGTTATCGCACTAAAGACCGCTTTGGCCGTTTCTTTCGCTTTCACTTGCGCTTTCCAACGCTCAACTTTAAGCAAAGCTTGAGTAACAGCGGCGTTTTCTTTATCAATAAACGGGGATTAGTTAAAGCTACTTTTAGTGCTGGCGATGATGCCTTTAAACGTGACCCGAAGATAATGCAAAAGGTGTTTGACACTTTTCTAGAAAGTTTTAGTTGATTTTTAGACAAGAAAATTAACCGCCGGATTGCCAGAATTTACGCTGAAAGTGATAACAAGGGTAGTCCTTTCCCAAAGGAAGGAGATGATGAAAATACTAAGGGGGATGACAACAGTAGTGAAAAAACGGATACAGTCTCTGTATCAACTAAGTTAAAAACAACTGCTGATCAATCTGAAAGTACTCAGATGTCTAGTGAAAGCACTGCCACCGGTATTTCTAGTGATCCCCAAAGTCAGGGCAAAATGAACAACAAAAGTGAGGAACAAAAAAAGAAAGAAAAAGAACTCACTTGACAAATCTACCGTGATATTAAGTTTCTCAATTCCATTTATTTCTTACAGGTAAATAACGAACGTTTGTGGGACAATGTAGCTTTTTTAAATAAAGTGAACGCTGAAGACCAAAACGGGGATAAAAACCAGTTTGCCCAAGCAATGGCATCACTCAATAAGCAAATTAACATGTTTTACGTGTTGAATGAAATTACCAACAAGGAACACAATTTGCCAACTAAATACGGTGCTTATTCTGATCAATTAAAAAAGGTCATAAACGAAAACTTTGAAAAGATAGAAAATAAGAAAAAGGAGATAGAGGAAAAACAAAATAAAGAATTTCAGGAAAACAAGAATGGTGCATCAAATAATCAAGACACAAAGCAAGACGCACAAAAAGGTGATACCAATACCCAAAGCACTGAGTTAAAAGCTCGTCAAGCACAACAAGTGCAAAAGGATCAACAAAATGACAGTAAAGGCAATACTGCTACAAACAGTGATACTGGTAAATCGGACAATAAAACCGATACCACTGAAGATGAAAAAAACACATATAAACCCTTAACAGCTAGGGAGAAAAAAACCAAAATTAAGGAGCTTACTAAGGACATCTTCTTCTCTACAGCGCAGAACTATTTGTTCTTAGTGCAAGGTGGGTATAACAACAAGTTGTTTGCTGGTGGTTTACCAGAGCTCCACCAACGCTTCTTGTCAGACGTAGTGGTTGATTTCTCTGAGGAAAAACTCGTTTATGAAGTACAAGGTGAACCGATTGTATCCAGTGTAGCCACCATTGTGATTACCCTATTTTTAACAGTTGTTTTATTAGCTATTGCCTTTTTCTTCTTTAAATACCGCAACGTTAAATAGTTATGTCTGTTACACCAATTGTTGAAGTTAAACATTTAGAAAAGGAATTTGGTTTTTGACGCAAAAACCGTATCTTAAAGGATGTGAATTTTGCCATTATGCCCGGTGAGTTCCATGCCTTTATTGGTCAAAATGGTGCGGGGAAAACTACCACCATTAAGTGTTTAATTTCATCTTATCAGCGCTTTAAGGACGAAATTAACATCGATGGCATTAGCAATAAAAACGCCAAGTCTAAGGGTGTAATTAGTTATATCCCTGAATATGCTGTGTTTCCCAAACACCTCAATACCCATGAATACCTCTACACGTTGGGTAAACTGTCAGGTTGTAGTTTGCAAACCATTAAGGAAAAGGTTGATTACTGACTAGCACGTTTTCAAATTGAACACCTCCGCTTTAAAAAGCCAAATGACTTTTCTTCTGGACAAAAGAAAAAGGTTTTATTAATCCAGGCCTTATTTAATGATCCCAAGCTGTTAATCATGGATGAGCCCACTGCTAACTTAGATCCCAAAACACGTAATGAGTTTATGGATGTGTGTTACGAACTTAATGTACGTAACAAGATGGCGGTGTTTGTTTCATCCCATATTTTGGCTGAATTGGAAAACTATTGTGATTCGCTCACTGTTATTCATGAAGGGCAAATTCTTTTTAATGGCAAAACAAAGAACATTGCCAAAGACAACTTTGGTTACCGTTTAAAGGTTAACCATTCCGATAGTTTACGTAAGTGATTAAAAGCACAAAAAATTGCTTACAAGTATTTCCCTGCAACCGATGACTTTCAAGTTGACTTAAAACAAGAGCAATCCAACAAATTTGCAGTTGAGCTTTACCAACAACCTGACTTTGAGGTATTTATTTTTGCACGACAAGGCAACTCGTTGCAAAACATTTACAATAACCTAATTGAGCAATACGAGTACGATCAAGCCCAGCGTGCTGTTGCTATCCGTGAACAAGATGAAGCTGTTTAAAAATTGAAGCGATGTTAACAACGTTAAACAACGGCAACTTTTACTAGCTTACTTCCGTTTTATCTTAAAACAGACCGTTAAATCTTGACTTTTATGAGTTACTGCTGGACTAGTACTGTTTTTACTGGCTTTAGTGCTGTTAATCATCCCCGCTTTCACCAAACAAGATCCTTTGTTTTTGTGATCACACCCAGTGGTGCAAATGTCGAGTTTAATCATTCCTTTCATTGCTCTGTTTGCCACCATTATTACCTTTCAAGTCTTTATTAATGGTTATTACAACGGTCTAGAGATCTTATTAATTACCCGCTTTTTTACTCGCGGTCGGTTATTCTTTGCTCGCTTAGCAGTCTTGTTTATTTGGATTACTGGCACGGCATTTTTAAGTGGTCTTTTTGTTAGTTTAACTGCTACTTTAGGTGCTACATCACAAACAGTTACTGACCTTGTTTTAAGCGTCTTTTTTGGAGTGCTTTTACTTAGTTTATTGTTTAGCTGTGTCTTGATTATTATCGCGCAGTTTTTAAACCGCACCCAAAGTATGTTGTTGTTACTGTTAGGGGTTTCCCTAGCTAGCTTTACAACCGTTATCTTGGCCTTTTCAATTAAGCCACCGAGTGAAAATCTCCGTGATAATAGTTACCAACCACTAAACCTAAGTCTCATCAGTAAGTCTAAGTCCAAGGGTGTCGAAAATGTCTTTACCATCATTGACTCTTCAAATGTATCCGATCCGCGCAATAAAAAGAAATCGATTGTAAAAACCAATCCGCAAGCGATTTGGGATGAATATGGGGAGAGTAGCTTCTTTCAAAGCCAGTATTACTGGAATGTAGGGTATTGAATTAACAGTCTGTTTCGGTTAAACAGCCTCAGTGATTACCGCAACTTTGATGTTAACCTCTATTTTTTAAACACTAAGCTTAATTTTGACAGGGAAGTAGATACTGATAAAATGGTCGACTTTGTTAGCTTTCGCGATCAAAGACACCTTTACTTTTTAAGTTATTCCTTTTTTATCAATACCCATAACCTGCCCACACAACCGTTACCCCGGATTCTTACTTATGACAATAACGGTGCCTTTTTAGAGAAAATAGTCCCTTCCTTTGATGACATAAAACTAGATCCACCCCGGGTCAAAAAGGTTTACAAATTCTTTAGTGACACCATTATCCAGTCCTTCCGTGATTATGAAGCGGAGGAAAAAGAAAGGCAGGAAAAGGAAGAGAAAGAAAAGGCTGAAAAGGACAATGGCAACGGCCAGGATTCCAATAAAGTTAACTCTGTCAGCACGGAGCCTAACAATAAGAATTCATCAGATGCGGATTCTAAGGATAACAATGATTCATCCGATTCCCAAGGCAAAGACTCAAGTAAGAGTAAGCCTAAGTTTAGACCTAGGCTGCCACAATTCTTTGATCGTGTTTCCATTAACTACAGTAAGTTTTCCAGTAGCTTTAACACACAACTAAAACACATTCATCAAGGACTTTCTACCCGTGATGAGGCCATGGAAATCTTTAAAGATAAGGTAGCGTTATTTTATGCCCTATCAATGGCTTATGACAACTTTACTTTCCAAAAGGACAGTGGCATTATTGATAACACTAGCTTAAACAAATTTAAAACAGAGTTTAACAAGAAGCAAAAAGAGCTAACTGAAAAAAACAAGCAAAAACAGGACGGTAAAGACCAAAAGAGTCAAAGAATGACACAAGGTGCTGATAAAGCAGTCACTGTTGAGCCAAAAGCTATGCAGTCTGAAATTGGAATGACTGATCAAACTAATGACCAAAGCAGTTCGGAAACAAAGGACAGCATGGATAGTTCAGACAGTAGTGATACTGTTGATAACACTGATGAATCAGAAGATAAACAAAGTGAAGAGGAGGAAAAGTTCGACGAGGAAATTGAGAATGCCAAAAAAATGCCTAAGGCTGAAGACGCCTTTTTTAATACTGCCTCTATCTGGTTGAGCTCACCATTTCTCTTTTTTGAAAATGGTGCAAAACGCGAACAACGATACGAAATCCATTTGCTCAATTCAAACACACAGGTTAATAACGAAATAATTAAAACTAACAGCTTTTATTACGTTAGTGGTGAACCGATTGTAGGCCAAGAAGTCATTATTGCAATGGTGCTGGTGGTAACACTAGGTCTGTTAGTTGGTAGTTTCTTCGCTTACCAAAAACGCGATATTAAGTAATTAGTTTTTCTGTAACGACCTAACGTACTTCAAGGTAATTTGGTAAGCTAGTTTTAAATCCTTTTTCGCTAGTTTAACCAAAGCGGTAATATCAATGCCTTTAGCTCACTCTTCATGAACGCGCATTGGTTCTAACTTATCCGCTAAGTAGATAATCATGTCTAGTTGACTCATCTTTTGTGGTGGTACAGTGTGGTTTCAAATAGCGCTAAAAACAGCACTATTATTTAAACCATACCAATGCTTTAAAATGTATGCACCTACATAGCTGTGTAACACCTTTCAGCTAGGGTAATCATTTACACCCGCTACTTTGGCTAATTTCTCTAATTGTGCTTGGGGTAGTTGCTTTGCTAGATCATGGTAAGCACCAGCAGTATAAGCCGTTTTACCATCGAGTTTATTTGCCACTGCTAAAGTTTTAGCTAACTGACCAACGCGCAAACAGTGTTGCATGCGCGGTTCATCTAACAGTGTTTGCAAAATGGTTTTTAGATAAATGTTGTGTTGGTGGATATAGTGCAACACCTTAGCGGGAATTTGTCGAAATTTATGACTACCAAACATTTCACTCGATGCCAAATCTAAAGGACACTTACCCAACAGTCGGACGTTAAACTGTTGTACCAATTGTTCATCAATTGGATAACCGAAACGTTCATAACAGACAAAACGACAAAGTTTTTGTAACTGCTCAATGTTATGTCATTTGGCCAACTCGCTTAATTTATCACTGCCAATTAAAAAGTACAACTCAGCATTGGGAAAGCGCTGCTTAAAGTGTTGTACTGTATTGAACGAAAAACCGTTTTGCGCTTTAATGTCAAAGTTAGATACTGCCATCTGAGGCAATGCTTTTAGCGCAACCCTTAACATTGCTAAACGTGCTTGATTAGATGCTTTAATCGGTGATTTAAAAAAGGCTTTAGCAGTTGGTACAAAGTAAAGCTTTTGTGCTTTAATGGCTTGTACAGCACGCTTAGCAATGTAGATATGCGCTTGGTGGAGGGGATCAAATGCACCACCAAAGATCACTATTTTTTTTCTCAAAGAGCTTAAATTGTTTAATTCTAACTAAAATAATCCTGATAGCTACAGCCATTTGTGCTGTCCAGGTCCATGGTAAAAAAGGAACTAGAAATTTACAACCTCTATACTTTTCAAATAGACCTCGATAAAAAGCTTTTATTTGAAAAAGCAGATAATCAACAGAATTACTCTAAGATTAGAGCTCGTTATTTTAAAAATTCTGCACGTAATCAACAAGCAGTTTTTCTCAATAAAAACCTGATTAAAAACACTTTTAATAAGGCACTGTTAAACTTCTCGGATTTTTTGAGTGGTAGTGGTGTAGAGAGCATTTTTAAGCAGGTCATTGATGATCAACCTGAAGTTTTAAACTACCTCAAACAGGTCAAAAAGGAAGATAGTTGCGATGGTCACAGCGAAGCGTCACAGTTAGTATTTAATGTAGTCATTAACCCTAAAAATACGCTCGCTAACTTCTTTGAGGAATTAACCATTTACCTCCACTTTAATGAAGAGAACAGCACAGTTGTCGGGAGCTTTTCGTTAAAGTGAAACATTAAGCGTGCCGACCTGTTTTCAGAAACGAAAAATATTGCCATTAACAATCTGATTCACACCTTCTGTAAAAACAACTTAAACGAGGTTAGCTTTATCCAGATCATTAAATGCTTTGCTAAAACACTGATTAATAAGCAAGGACAAATTGTGTTGGAGAGTTGTGCCTTTAAGCAAAAATGACAAAACATCGTGGAGCAAAAGTACCCTTTTTCCACGATTCACAAAAACTTAAAGATTGTCAATTCTGATTTCTTTGATGCTTTTTTTGTGATCTTGTTGTTAATTTGTCACCTCAATAATAACTTGTTGTGGTTGTGTGAAAAAACTGAGCACTTTGAATGAAAGCTCAACAGCAAGATTAGTAATTTCAAAGAAGATAACACTGAAGTGTACTTGTCGAAAATGCTGTTGTTTTTAAAGGACTGGTACTTTGAAAACCAAGCCGTTACTAATGAAGACATTGAAAAGGTTGATGAAGTGGAAGACATTGGTAAGTTAGTGGAAAAATACAGCGCTAATCAACCACAAAAGTTGTCTTCTAATTCAACCGTTTATGTATTTGAACCAGACAAAAAACAGTGCTTTTTAAAGAATGATGACTTTTTCAACACTAACGAAGCTAAGCTGTTGTTTTTAATCACAATGCAACCCAATGTTTTTGGTCTGGATGATACTGCCATTGCCAATGACTTAAACCTAAGGGAAATTGGTGACTTCTTTAAGGAAATTGACTTTACTGACAGCGATGTGCTCAATGACTTTCAACAACAAAAAGAAACTTTGTTAGTACGGCGTACCTTTAACCAACTGTTGTTTATGAACAGTAATACCGATGTTTTGAGTATTGTTAACAATAAGTTCAAATCAGCGATCCATAACATTGTCTGAACGATTACTTACAGTAAGGCAATTATGCTCAAGGCATTTGATTACTCCAAGATCTTTGAGCAAAACCGCACTAACGATCCTTCTCTCTTGCGTTCTAACCTCAACAGCATTAACCGGCTGCGGTATCTCAGTGAATACTTCCGTACCGCTTCAGTTAAGTATGACCAGCTGTACACCAAGGTAAAGGAGTACATGCAATTAGATCAGTTCTTAGTAGACATGATTAACCAGGTCAACCATGAAGATGAGATCTTTGGTAAATACAAGGAACGGGTTTATTTGAGTTTGGGAATAGTTACTGCTGTTGTCTTTGGTATTATTGAGTTCTTTAACTGTGTTTGAACGGTTTTAACAGTCAGTCAACAAACGGCTGAAAAAAGTTTAGCTGATCCAAGAAACACGGTAATTATTGGTATTGGTACTATCCTTGTTTTAACACTGTTAATTACCATTTTAACCTTTATGACAAGACGGCTTTACCTGTTTGAATTTAACAAGAAGCATAAATAAAGATGGAATTCAATAAGTTACAACTCAGTCACTGTATTTCCTTTTACATTTCAGACGTTTCGGAAGTGTTCTTTGAAAGTATTAACCAACACCCTAGTCGTGATTTTGTCAACAACATCCTGCAAAAGATTAAGAGCACTCTCAGTGAAGAGGAATTGGAAAAGCTCAACACCATTGAGGAAGTTACTAAGGATGAGAAAATTTTGATCATGCTCAACCATGTGTTGAAGAAGATTGTTTCTAAAACGGGTAGTTCTAGCTGTGACTTATTTCAAATAGTGAAACACGACCGCTTTAACGAGCCGGTTTACATCCAAAGTGTCAATGCCTTTGAAAACAATTTGATTAACAATGAATTTGCGGAAAGGCGGTATGACTATTTAATTGAAATTAATAAACACTCTTATTTAAGGAAGTATGTCAATGCCATTCGCATCTCCTTCTTTTTAGATTTAAGGGCGCAAATCTTGTCCGGTTCCTTTACTTTGGATGTGATTAACAAACAGTTGGAACACCAAAATAAGGAAGAGTTGTTCCAAGCGATTTACTTACGCTCCTTAATTAAGCATTTTATCTCGAACCAACCTTACCCGATTTCCTTAAATTCGTTTATTTTTGGTGATAAAAACCGTGAAAACAAGACGGTTGAAAACGACAAGCTTAGTGTGCTTAAAAACAATTGGAATCAAATCTTCTTTTCTAAGTACTTTGATTTTGTTGCCAAAAACAAAGAAGAGCGTGTTGTCGATAACAACTGCGATGAGCTGTTTTACGCCACCATGAACACGCTCTTGATTATGTTGATCATTATTGAAGAGCTCCGGGTATACTTCAACAGTAAAGAGCCAGCCTTAATCTTAAAGCTACTAGATAACAAGGTTTCACTGCGCGAAGACCCCGACCAAAATCCAGAAACTGATTTGCATGAGTTAATTAAGTTTGCTGAAAAGAATTACCTCGAAAAGGAAAAAACAAGTCGCTGACACAAAAAACGCGTCAAAAGCTTAGAAGAGTTACTAGAAGAAATTAAACAAATTAATTTAGAGACTAAAAACGAATCGTTAGCATACCCCGATGAAATTGTGGAATTAGAGTTAGATAACGTTCACAACTTTGTCTCTACTAAACAGGTTTTTCGTCACCAGTTAGACTTACAAACTTTACATGGTATAGTCATCAACCCTGAGCGCTATGGTATTGGCATGTGGAGTAACCACTTTGTCGATTGGGAGGAGTTTAAAGATTTAATTGAACAGATTACTGATGCTGAAAATGGTAGTGATCTCTATGGTTTTGAAAAGGACTTGGATGAGAGCATTTGCCAAGTTAACAAGAAGTACTTAACCTTTATTAGTAGTGACAGTAGCTCTTTCTTAATTATTAAGAATGACCAAACTAAGGTTATCTCTAATTATGTCTGGGCGCAACTGTACTTTGAAACGCGCCGGTGGATTATTAATGATATTGAATACGATCTGTATGAAAAGGGGTTTGACAAGAGCCACTTTGCTAGTAACATTGCCCTTTTAGAGAGCTTGAATTTTAACTGACTCGATCCCTTTTACGGATTGACCTCAATCAAGGAGATCATGCAAAAGATTGACAGTAAGAGTAATTTAAAAACATCGATTGCCGAAATGGTCGCTAAGTTTAAACACGAACAGCGCATCTCCAAAAAGGACAATGAGCGGGTGTTGATGATCTTTGCTTATGTAGCCGCTGCGGTAGTTGGTTTTATTAACTTTTTTTCGATGGTGTTTACCATTCTTACAGTCAGTGATCTCAATGCTGGTCTCACCCCCGCTAACATTGTTGTTATTGCAATTGCGAGCTTATTGGCCTTATTTTTAATTGTTATTGCTGTTTTATTTCGGTTTAGGTGAAAGTACATTAAGCACTAATGCAGGTTAAAGTAATTGATGAAAACAGCAATACAACCGCTGTCCTTAACTGCGCTAAAGCGAAAACGCGAGCGCTAGCTTGGTTGTGTGATACAGTACTGTTAGCGATCTTACTCGCAATTATTTACGGCATTAGTAGCATTTTCATCAAAGAACAGTCCAGTGTGTTTTTAATTATGACGGTAAGTCAAGCAGTCTTGTGGTTGACTTACTTTGTCATTTTACCGGGTTTATGGAAGGGCAAAACGCTCTTTCGTGCTTTACTGGGTTTAAGTTTACTCATCTTTAAAAAACGCTTTTGAAACCTCTTAGTGCACGAGCTTTTTCTGTGAGTATGGTACTCAGTTATCTTTCTAGCACTAGCAATTTACTTTTTTGTCAACAGGGATGATCCGAAGATACTGCAAGCTTTTTTTGATAACCAAAACAGCAATCTTAGCTGGATATTTGTCAAAATCTTGCTAAGTGTTATTAGTGTCTTACAACTAGTATTTGTGGTGTATTTCTGTTTCAGCTCCCAAAAGCAAGCGTTGCAAGATTTGTTATCTAAGAGTTTTATGGTGCAAAAAACAATTAAAGTAAAGGATTGTAAAAGTGAATTGAAGTCCACTAACACTATAAAAACGCACTCTGATTTACCCGGCGACATTGATTTAGAACAATTAGGTGATTAAGTTATGGAACATTTAAATCAGGAACAGAAAGCTGCTGTTACTTGTGATAACGGTGTTAACGTGGTGTATTCCGGTGCTGGCACGGGTAAAACAACCGTTATTGCTGAACGCTTTGCTTACTTAGTCAATGAAAAAGGCGTTAATCCTCAAAGTATTTTGGCCTTTACCTTCACTGATAAGGCAGCGAGTGAAATGCGCCAACGCATTATTAAGTTAATTCCACAAAAAAGCTTGCAGGATCTGCACATCTATACCTTTCACAGTTTTGCCAACAGGTTTTTGCAAAAACATGGCAAAAGTGACTTTGCCATCTTGAGCGATTCTAACCGCTTCTTTAGTGATTATGAAATGGGTGATCAACTACAAACAGTGGTGGAAATTTATAAAAACAAAGTAGTTGATTTAGAACTTGACAACCTAGAGTATAACTCCGCTTTTAGAGATGCTTGTACAGATACTTTTAACGAGGATTTTAGTACCATCTCCAATGGTCAGTTTCGTAAACGGGCTGCGACTGCTTTACGGGCTTACCAGAACTATTTAATTACCAATAACCTATTTGATTTTAGCGATTTAATTATTGAAACTTGCCACCTTTTAAAAGGTAATAGTGAATTGTTGCAAGCATTTACCGAAAGTGTGCATTATATCTTAGTAGATGAATTTCAAGACACTAACCTAGCCCAGTACGAATTGGTAAAGTTACTAGCTACAACTCATCCTAATCTCTTTTTGGTGGGTGATAGCAACCAGATGATTTACGGTTGGCGGGGTGCTGTTGTAGAGATCTTTGAACTTTTAAAAAATGATTTTCAAACAGTCAAAGAGTTTTATACCACCCAAAACTACCGCAGCAGCCAAGCGGTGTTAGCAGTAGCGAATGATGTACTAACCGCAATTGCTAGAAAAGAGAGGAAAGCGCTCGTGTTGTTGCACTCTAGCATTGATTCTAAAGCTGTACCGGTACACTATAAGGCCAATTCTTTGAAAAACCAAGACCAGTGGATTATTTACCAAATGAAACAGTTGCATTTAAACAATGGTGTACCTTATGATCAGATGGCGGTGTTATTTCGTAAGAATAAACACCTTGACGCTTTTAGTCAAACAGTGCTGGAAGATGGTGATTTACCTTTAGCTAAATTAAACCTTTTAACAATTCACGCTGCCAAGGGTTTGGAATTTGAGGCAGTATTTGTTTATGGCTTGGTTGAACGCGCTTTTCCTAGTTTGCACTGGGATGGAAGTGATAAGCACAAGTTACTAGAAGAAATGAAACTGTTTTACGTAGCGATTACTCGTGCTAAACAGTTTTTATTTTTAGTTTCTGTTAGTGTGGAAAGTTTCAATGCGTATTATGAGCCGTCACGCTTTTTAAAACTAATTGAAAAGGAACATTTGCAAACACAAAAGGCAGCGTACTTTAAAGAACAATTAAAGACACAGCCAAAACCAGTTAATTTGTATACAGAGACAGAAAACGCGGAAAACTTACAAAAAGCCACTGACTCTAAAAAGTGAATTATTTTAGGGGCGATTCTTTTGATAATTGTGATTATTACCGCTGTGTTAAAACTTTTTGTTGAAAATTAACATTTTATCCCTTTTTTATAACAGATTACACTAATTAAAAAAACAATCTTAAACCTAGTACTATGGCATTTAACATTAGCACTCAACTCAATAAGGAACAACGAGCCGCTGTTACCTGTGGTAAGGGTGTCAACATTGTCTATTCTGGTGCTGGTACTGGTAAGACCACCATTATTTCCCAACGCTTTGCTTATTTATTTAACCAAAAGCGAATTAATCCTAGCAACATTTTGGCGTTAACCTATACAAGGAAAGCGGCGAGTGAGATGAAGCAGCGGATTTTGGAGCTGTTACCGGAAAAGTATCATAAGGATGTCAACATTTACACCTTCCACAGCTTTTGTAGCCGTTTTTTACGTGAAGAAGGGCAAAAAAACTTTGTGATTGATGATGACTTAAGTAACTTTCTAAAAGACTTTCTCAAAGAGAGTGAACTAAAAAGTCAAAAGGTATTACAAATTATTGACGGTTTTAAAAACGCTTACTTTGATTTTGATACCAACAGCTTAAAGGATGATGAAAGATTGGTCGAGTTGTGTGAGTTTCATTTAGACCCCCAAGAGCGTAACTTTCAACTTTTCAAAGAAACCGCTATTGCTGCTTTTGTTGAATATGAAAAAGGTAAACAACAGAACAATAAAATTGACTTTGCTGATTTATTAATTAAAACTTGCACTTTACTGAGCAAAGATCACAAATTATTGCGCAAGTGATCGAAAAAATTTCAGTACATTTTAGGTGATGAGTTTCAAGATACCAACCAAATTCAGTATGAACTAATTAAGATGTTGGCCAGCCATCACCAAAACCTTTTTTTAGTGGGTGACAATAACCAGATGATTTACCGCTGAAGGGGCGCGGTTAGTGACATTATTGATTCTTTAAAAAGTGATTTTAAGGTAAGACCCGAAAACGAGTTTTACATTACCCAAAACTACCGTTGTGATCAGAACATCTTAACGGTGGCAAACTCGATTCTAGGCACCATTTATGCCAAAGAAAACCAACCTGATTCAACTAAAGGCTTTCTTTTTTCTGCCATTAAATCAAACCGGCTTCCAGTTTATTTCCAAGCCTCTTCAGTTGAAGGCCAACATTCTTGAATTATCAATAAGATTAAGAATTTACATAAAAACCATGGCATTCAATACAAAGACATGGCTATTTTGTTTCGCACCAACAGAAACATGGATTCGATGACTGAAGCGCTTGAAGCTGATGGCAGTATTCCCCTTAAGCAAAATAAGGGTTTTTTTAAACAGTTAGAGACTTTTAAAAAGGTATTAGTGGCACTCATAACTAGGTCAAACTATGACATTAAACTAGCTTTAAAAAGTTTGCGGGTTTGACCTAATGTTTTAAATAAAAGTCTCACTGTAAACGAGCAAGTGAATTTAGATAAAATCTTGCAAAACTTAGAGCAAGCCATTTTTTTAGATGAGCATACTCGGGGTGAGTTGACGGAAGCTGCTAAAGTTTTTACGCGTTTAATTAAGTTTGTTGAAGAACAACAGTTTGAAGCTTTATTAGCCTTTACTTTTGAGGCATTAAATACTGATCAGTTTGTCTGCAACTTTATTTTTAGAACATTGCAAAAACTGCAAACTGAAAATAAAAACTTCACCATTACCGATTTTATTAACGAGTTAAGGTTCCAACAAGATGAGTTAAAGCAGTCCAAAGATAATGTGATTAATTTAATTACAGTGCACTCTGCTAAGGGCTTGGAATTTGAAGCTGTCTTTATTTATGGGATCAATCAAGACAACTTTCCGCTCAAATCGAAGAATCAAATAATAGATCTACAAAGAGAATTGGATGAACTACGCCTTTTTTATGTGGCCTTGACAAGGGCAAAGAAATACTTGTTTTTACTTTCAGTTTATCAAATGAGTGGTGAAATTATTTACCCCTCTAAATTCATTCGTTTTATCAATAAAGAGGATAGACTAGAAATTGCCACCATTAACCATAAAGTTCACCAGGATGATGAATTCTTCGATTCTTCTAAAACAGAAGACTATCAAAAAAAGTATTTAACAGAAAATACGGATTTTGTTAATGGTGACTCTGTTAGCCACCGCACTTATGGTAAGGGCGTTGTTGTTGAAGTTAGAGAAGATGCAGTCTGTGTCGCTTTTAAAAACAGTAAGTACGGACAGAGGTGAATTGTTAAAAATCACCGTGATTTGGTGAAGGCTGTGTATTAGTTATGATCTGCTATTAAAAAAGAAATTGTAATAATAAAAATGAACAAGTTTTTACCAATTTAATAACTAAAATTAAAATCCTTATTAATTGTTAGTAATAACAACTTTCAACATAACTAAATAACTAAACTCACTATATCATGGAAAAGAAACGTACTGAACAACGTAATGGCGTTGAAAAGAAGATTTGAGAAATAGCTGATAAATTAAGGGGAACCATTGATGGATGGGACTTTAAAAGCTATGTTTTAATTGGTCTTTTTTACCGTTTTCTTTCCGAAAACCTATGCAAATATTTTAACGATTCTGAAAGAAGGAATAATCCTGATTTTAGTTACGAAAATTTAACAGATGATTATGAAGCAATTGATGCATTAAAAGATGCTGCCATTGCAAGTAAAGGCTTTTTTATTAAGCCATCTCAGCTTTTTCAAAATGTTGTTAAAAGTATTCGTGAAAATAAAAATAACGAAGATCTAAATACAACTTTGCGCGATATCTTCGATGATATTGAAAAGTCTACTGAACTAGGTGATGGAAGAAGTAAAGAATCCTTTAAAGGTCTTTTCAAAGACTTTAATGTTTCTGAGGTTAAGTTAGGTTCTACTTTAACGATCCGAACAGAGAAATTAAAAGAACTTTTAACAAGCATTGACACAATGGAGCTCGATGAATTTGAGAAAAATTCTATCGATGCATTTGGTGATGCATATGAGTTTTTAATTTCCATGTATGCACAAAATGCTGGAAAAAGTGGGGGTGAGTTTTTTACACCCCAAGATGTATCTGAACTTCTCGCTAGAATTGCGATTGGCAAAAAGGACACCGTTGATGATGTTTATGACATGGCTTGTGGCAGTGGTTCTTTACTTTTACAAGTAATTAAAGTTTTAGGTAAAGAAAAAACTTCACTGGTTAGCTATTACGGTCAGGAAATAAACCACACAACTTACAACCTTTGTCGGATGAATATGATTCTCCACAATATTGATTACGCTAATTTCAATATTATTAACGCTGATACTTTAACAACGAAGGAGTGGGAGAAGCATTACGTAAATTGCTCAAATGAAAATGGGTTTGAAGTTGTGGTTTCCAATCCACCATATTCCATTAGTTGAGCTGGTGATAAAAAATCCAATTTAGTAAGTGACGTTCGTTTTAAAGATGCAGGGACATTGGCACCTAATTCAAAAGCTGATTTAGCTTTTGTTTTACATGCTCTATATGTATTAGGTCAAGAAGGTACAGCAGCAATTGTTTGTTTCCCTGGAATCTTGTACCGTGAAGGTAAAGAGCAAACCATTAGAAAATACCTAGTTGATCAAAACTTTGTTGATGCAGTGATCCAATTACCAAGTAATCTTTTTAGCACAACATCAATCGCTACTTCAATTCTTGTTCTCAAGAAAAATCGTGATAAAAAAGATCCCATTTTCTTCATTGATGGTTCTAACGAATTTGTTAGAGAAAAGAAGAATAACCGCTTAAGTCCAAAAAATATTGAAAAGATTGTTGATTGCTTTAATAGCAAAAAAGAAGAAGCTAATTTTGCAAAGGCGGTTGAAAGGGACAAAATTAGGGAATCTAACTACGACCTTACAGTAGGTAAATACGTAAACTCAGAGGCTGAAAAGGAAGAATTAGATATTAAGGTTTTGAATCACTCTATTGATGAAATAGTAGATAAACAAAAAGATCTAAGAACTAAAATTAAGGATATTATTCAGGATATCAAAGTAGATTTTGACAACATTGATATTAATAACTAGTTGATAATAAAGCAATTCTTTGTTAATTTACAAGAATTTAAAAACTAATAAATTAATTTCATTATTGTTGTGCAAATTAAAACTTACAAAATCAAGGATATTTGCGAGATCTCAAGAGGCAAAGCAATTACTAAAAAATACATTAAGGATAATCCTGGTCAATATCCGGTTTATTCATCTACTACTGCTAATAACGGTGAAATTGGCAGAATAAAGGATTACGATCTTGATGGCGAATACGTTACCTGAACTACAGATGGTATATATGCAGGTACTGTTTTTTATCGTAACGAAAAGTTTAATGCTTCTCAGCACTGCGGTGTTTTAAAACTTAAAAATAACGAAATAAGTGCTAAGTTTTTAACTTATGCATTAGGAATGGAGGCTCCTAAATTTGTTAATAATGCTTGTCCTATTCCTAACCTCAATTTATCAAGAACTGAGGAAATTGAATTAGATTTCCCACCCTTGCAAATTCAGCAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATAACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGAACTACAAATGGTTATGCTGGAGTAGTTTTTTATCGTAATGGCAAGTTTAATGCATCACAAGATTGCGGTGTTTTAAAGGTTAAAAATAAAAAAATCTGCACCAAATTCCTATCCTTTTTATTAAAGATAGAAGCACCAAAATTCGTTCATAATCTAGCTTCAAGACCAAAACTAAGCCAAAAAGTAATGGCTGAAATTGAACTCTCTTTTCCACCTCTAGAAATTCAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCCGAAATTGAATTGCGTAAAAAGCAATTGGATTACTATCAGAATTTCTTATTTAATTGAGTGCAAGAACAAAAGAAGAACTCACTTTCTACTAATCTAAATTAAAGGTTATTTCTTAACCTTTGAGAGTTTCTTTAATTAAAAAAGAAATTTCTTAATTTTGTTGACATTTCTAATTAAGCCGTTCAATTTATTTAGTGTAAAGAGCGCATATGCATTACACATATAAATTGAAAATCGGGCACTTGTTAAATTTAGCTCAATTCTTATACAAAATTGAACGTTTTAGTTATTTTTTAGATTCTCCAAAGAAAGGAGGGATTTTTTATGCCTAAAATTATTTCTAAACGAGAATTCAACATTCTTAGAAAAACAAAAGAAGAAATCTTTTCTCAAATTGTTTACACTAAGAAGAGAAACGGTCTGCACAAGGCTGCCAAAGAATATTACTTCAAGGACAAAGACTTTACAATAATAGAAGATCAACAGGATCGACCTGATAAACCTGAGGAACTGGACACACCGGATATTCCAAAGCCACCAAAACCTCCCAAGGGTCCTGATCAACCCGAGGAACCAGGCCAACCAGGTGGTCCCGATGATCCTAATTCAGGAAATAAAAAGATGCCAAAGCCAGACGAATTTGTAACCCACAGACAACTTCAAGAATTTAAGAAGGATCTTTTAGTGGAACTTCACGAAATCTTTCCTACAAAACCTGAATTAAAACGGGTTGAAGAAAAAGTTGATGTTCTGTTTGAACTTCAAAAAGCTCAGGGTGAACAAATAAGAATCCAAGGTGAACAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGGGCGAAACCCTACAGCTAATCTTGCAAACACTACAAAAGATGAACGATCGTCTGGATAAGATCGAAGGTAAAATGGACAAAATGGAAAGTAGAATGGATAAAATGGAAACTCGCTTAGACAAACTAGAGTCCAAATAAATCTAATTGAAATAAGAATAACAGCAGTTAATTTTTAGGTTAGCACGTTGCTAATTTATTCATAAAAGAAGAACTATTATTAGTTATTTATTCAGCTTCTTCAGTGGTCTTTAAAGAAATATTAGTATTTAATAAAAAAATTTATTGTTGTTAAAGTTTAAACTCTAGTATTTCTTATTTATCATTAAGTTATTTAATTAACATTAAGTTATTTAATTAACTTATTTAACTTAATCTTCACATGGAAAAACTGGATTTTTTAAAGATAAAAAAAGATTGAACTGTACCAGTTGAATATCAACCTAGTGATCAAAAAATAGAGTGACAATCTTCAAGAAAAATAGTTCAAAATTTAAAAAACAAACTTTTATCCTTGGGTTATGAAGATATTAGCAAGGGTTTTGACGAAATTCCTAAATTCTTAGAGAAAAATTTAAGAAATCAACTTAGCTTATTAAACGATTTTCAATTTTCCGATAGAGAGTGATCAGATTTTTTAAATAACTTTATTTTTCAATTAGGTGACGGCAAAAAAGGAAGAATTGAAAAAATCCAAAAAGGTGAGCTTTATGAACTAAAAGATGAAAATGACAGAATCATTAAAAATGTTCGTTTAGTAAATAAAAGAGAACTATCTAAAAATAAATTGCAATTTATTTGCCAAGATGAAAGGAATGAGGTTATTATATTCATCAATGGCCTTCCTTTTGTTTTAATAAGTATCTTTGAATTTAATTCACCACTTCAATCAAGCTTTCAAGCATGAAAAATTATTAATTCCCTAATAGATGACGAAAAATTTAAAAAGTTTTCCGATTACTTGCACCTTTTTGTTTGAACAAACGCAAAGGAAACAAGATACTGCTCAATAACAACATGGGATAACCTTAAAGAAAAAAGGAAATCAGTAAGAGGATTGTCAAACTCTTCTTTTAAAAGTTTGATGAGATGAAGTGACTCTGAAAACAATAAAATCGACTATATTGAAGACTTTGCAACTCATTTTTTAAACAAAAACGCCTTATTAAACGTTATTTGTAAATTTTGTGTGTTCAGGTCAAATAGTGATCTTTGAGTTATGCGTCCTTACCAAATATGCGCAACTGAAAGAATTTTAGAAAAAATAAAAGAAGACAATAGGAACAGTAAAAACTCTAAAAATGCAAGTAAGGGCGGCTGTATTTGACACTCAACCGGAAGTGGTAAAACACTAACTTCTTTTAAAGCTGTTCAATTGGCTAGTGAAATAGACTTTGTTGATAAAGTCCTTTTTCGTTGTTGACAGGAAAGACTTGGACAACCAAACGATCGAAGAATATGAAAAGTTTCAAGCGGGTTCTGTTAGCGAAACTGAGAACACAAATGATCTTAAAGAAAAGATATTAGACGATAGTACCGCCACACGTGCAATTGTTACTACTATTCATAAACTTAAGAGGTTAATAGATCAGAGAAGTAAGCTTAAAGACGAAGATCTTAAAAAGAAAAACATCGTTCTTATATTCGATGAATGTCATCGATCTCAATTTGGAAAAATGAAACAAGAAATCGATGAGTTTTTTTAAAATGGGATTTTATTTGGCTTTACTGGAACGCCTATTTTTGAAAGTGAATATTCAGACAAGAATCAAACTCTAGAGAACCAGCAAAAGACAACTAGAAGTAAATTTGGTGAAATTATTCATACCTATACAATGTCTGATGCTTTGGATGACGAAAATGTACTTTGTTTTAGGTATAAGAGTTATTACATTAAAGATGATGGAAGCCGAATTGATACATTAAATGAAGAGAGAATTAAGCATATTGTTGATTTCACTTTAGATAAATATCTAGATGCAACACAAAATACTCCGAAATTTGATAATTTTGAAAATCCTGTAAAAGAAGGGTTTAATTCGATTTTTGCCTGTGAGAGTAAAGAAGCAGCAATCGAATACTATAAAGAATTCAAAAAACAAATTGAACAAAAGAAGCTACCAATTAAAATTGCAAGCATTATAGCGCAAACGAAGGTAGTGATGAACAAGAAATTAGTGTAAAAGGTCTTGATAAATCCTCAAAGAGTTTTCTAAGTCAAATCATTTCTGATTACAACGAAACCTTTCAAACAAACTTTAGCATTAACAATCTAGATGAATATTACAAAAATGTTTCTGATAGATTAAAGCCAAAAAAGACAAAAAATTTAGATGGTGAAAATAAAAACACAAATGGCTGAGAAATCGACATTTTAATTGTTGTTAACATGTTCTTAACTGGTTTTGATGCACCAACCCTTAATACACTTTGAGTAGATAAGAATTTAAGCGAACACACTTTAATCCAAGCTTTTTCAAGAACTAATAGATGTTTTGGAGAAACTAAAAAATTCGGAAACATTTTTAGCTTTAGAGATATAAGAGAAAACTTCGATCAAGCTCTAAAAATGTACGCAGACAAGCGTGGTCTTAAGGATATAAGATCCAACATGGTTATGCGAGAATTTGAGGAAGTTTTTGAAAAATTCAAAGATTGAACCGAGAAAATAAAACAAAAATACCCAAATCCAGATGAAATTCTTAATTTAGAAAAAGAAGAAAAGTGAGATTTTTCTGATCTTTTTGGTGAATTTCTTGGAGATTACAGAACTGTAAAAAGCTATCATGAATTCGACAAAGACAACCCTTATCTTCCAAGTGATGAATTCAATAAATATTTTGCTGCGTTTTCTCAAATTCAAGAAGAAATAAGGAAAGAAAAGGCAGAGAAAAAAGCGCTAGAAGTACAAGAAGAAGATTTATCAAATGAAGTACGAAAAGAACCGCAAATCTTTTCTTGAGAATATGGTGAGGGTATTCAAGCCAATTTAGATTACATTTTTCATCTAATTAAGGATCTCTATAATTGTGATAATGAGCAAGATAGACAAAAAGTATTAGACAAAATTAAGACTGCAATTAGAACAAATCCAGAAATTCAAGTGAATGAAGATATTCTTAATGAATTTATAGAGGACTTAGGGAAGTCGTTCGAGAAAGAAAAACTTGAAGAAGTTTGAAAAACAGAAGGTAAATTACGTGAGTGTTTAAAGGATTTCTGTAAAAAGAAGGAAGAAAGCAATAAGAAAAAAATTATTAATGACTTTTTAGTTAAAGGAGTTTTCCTTGTTGAATGAGTAGCTGATAAACAACTAAATACAGCTAAAAAGAAAGGTGAATACAGTCCTTCTTGATCTGATACAGAAAAATTAATTTGAAAAGAAAAATCAACCAATCTTCCAACTATGACAGAAAAAGATGAAGATGGCAATTTCAAGTGAAGAAAATTAATTACAGATATCTCGGAAGCTTTTAAAAATCACTTCGAAAGATTTATCCGCTTTGTTGAAATTAGATAGTTTTAAATTATTATCTATAAATCAGGTTTTCAACCATAGATTACTATGGAAAAGAAAAATAAAAAAAATACTGCAACCGTTCCCACCAAAGAACAATTGGATGGCATGTTTAAATCTCTTGAAAAAGAAGAGAACTATGATGTCTTTGTTCAAGTCATAAAAGCTTTAGGTAAGATGTAGTTGTGCGTGAAATAAACGTTTATTATTTAAGCAACGACTCAAAAAGTGATAAAGACGATATCGATTATTTAATAGAAGAACTTTTAGTTGAAGGTTCTCGTTTTAAAAATAGTCTTGTTACGTTTTTAGAAAAAGAAAAAGACTGCCAATCTTGCATTTGTTTATTTAAATCTAAGCTTTCTTCTTCATTCGAAATAAGAGTCAAATTTAATGAATTAAATTTAGATGCAAGTGATGAAAAAACTAAGGAATTTTTAAGATACATAGTTAGTAAATCAATAAACAATGCAAAATTCCTATCTTCTGAAGACTTTGATGACAAAAAAGAAGACGAAATTCTAAGCATCATTGAGAATACCTTTAATAGTTATTGCTACAAAAACGATTTACTAAGTCCAAGTTTGTATTGTAAAGAAGGCCGCTTGAACATAATTGATTTAATTAACGATATTTTTTGCAAGGTTTTAACTTTTCATAAATTGTCGAACGGAAATAAAAGACTAGCTACAGCCTTATTAGTTAATTTTCTTTACACTTTTGGATATTATTTCAAATGAACAATACCTCTTAAAAAAGAGGAATACTTTAAGTATGCTCACATGATCAAAAACTTTGTAGTTAGTTTTGAAGAACATAAAAGAGAAAACAGGGATGAATTTCTAGCAAAGCAAACAAAGAAATTTATCATGGACAACATCCTTTTAGCTCTCAATTTTTGAGAGACTAAATTAAATTAGTTTTTCTAAAAAACAACAATTAGCTAAGAAAAGTTATATGGACAAAAATGCCTTAAGAAAACAAATTCTGCAAAAAAGAATGGCATTAAGTACCATTGAAAAAAGTCACTTAGATCAAAAGATTAATCAAAAATTAGTTGCTTTTTTAACTCCTAAGCCATGCATTAAAACAATTGCACTTTATGAACCCATTAAAAATGAGGTTACTTTTGTTGACTTCTTCTTTGAGTTTTTAAAGATTAACCAAATAAGAGCTGTTTACCCCAAGGTAATAAGTGATACCGAAATTATCTTTATTGATCAGGAGACAAATACATTCGAACCTAATCAAATAGACTGCTTCCTAATACCATTGGTAGGCTTTAATAAAGACAATTACCGTCTAGGCTTTGGCAAGGGCTATTATGACCGTTATTTAATGCAATTAACTAGACAACAACCTAAAATAGGAATAGCGTACAGTTTCCAAAAAGGTGATTTTTTAGCCGATCCTTGGGATGTACAACTAGACTTAATTATTAATGATGAATAGCATTAAATTCATTTTCCTAGGTGACGTCTATGGCAAGGCCGGACGTAATATTATTAAGAATAATCTAGCCCAACTCAAATCAAAGTATCAAGCTGATTTAGTCATTGTTAATGCCGAAAATACGACTCACGGTAAAGGACTTAGTTTAAAGCACTATGAGTTTCTAAAAGAGGCAGGCGTTAATTACATTACTATGGGTAATCATACCTGGTTTCAGAAGCTAGATTTAGCTGTTGTAATTAATAAAAAGGACTTAGTGCGTCCTTTAAACTTAGACACCAGTTTTGCTTTTCATAACTTAGGGCAAGGTAGTCTTGTATTTGAATTTAATAAAGCAAAGATTAGGATAACTAATTTACTGGGTACTAGTGTGCCCTTACCCTTTAAAACAACTAATCCCTTTAAAGTTTTAAAAGAACTAATTTTAAAGCGCGATTGTGATCTTCATATAGTTGATTTTCACGCTGAAACCACCAGTGAAAAAAACGCCTTTTGCATGGCTTTTGATGGTTATGTCACAACCATTTTCGGCACCCACACCCACGTGCCTAGTGCTGATTTAAGAATCACCCCCAAAGGTAGTGCTTACATTACCGATGTCGGGATGTGTGGTCCGGGATTTGGTAGTGTTATTGGTGCTAACCCCGAACAGTCGATCAGACTCTTTTGTGCTGGTAGCAGAGAACACTTTGAGGTATCTAAATGCGGAGCACAACTCAATGGTGTCTTCTTTGAGGTTGATGTAAATACAAAAAAGGTGATTAAAACAGAGGCGATTAGGATTGTGGAGGACGATCCTAGATACTTAAAACAAGACTACTTTAACCTAATTTAACTGGGTTACCTTATTTTCTGTTTTATTCAATAAACGATGAATGTTTTTGCGGTGCAATCAAAACACCGCAATTGTTAGTGGTCAATACCACAATACAAAGAAGAGAATAATGTACCAATCATTTTGGTAAGAGATCGCGTTTATTGGATTAGGCTTAAAAAAGTAAAGGTAATCTAACCAAGGCACTAGGATAATGATCGCTAAAATAAAGAAGGTAACTAAGCTAGCTAAAGAAACGTACTTGGTTATTAAAGTAACTAGCAATCACAAAACTAAGCAAATAACAAATCACCACAGCGAAATTGCCAGCAAAGAACCGCCTGTAGTAGCGATGGCTTTACCACCTTTAAACTTAAAGTACAGTGGAAAAATATGACCAATGGTAGCTGCAAAACAACTTAAATAAGTGAGGTAATAAACCTTTTGATACTGGTTTACATTAAGGTATTGTTGTAACCAAAAGCTAAAGATTAAAAAGCTGAAAACAAACGCCAAAAACCCCTTTAAAGCATCACATAAACCAACTAAGAAGCCTATTTTCAAACCAAAAACACGGATTGAGTTAGTAGCCCCCGGATTCTTGGAGCCCAATTTCCTGACATCTTTGTTTAAGATCTTGCCAAAAACATCAGCAAACATCACTGAACCCATTAAATAGCCAATAACAAGGCTAAAGACAATAAGTAGAGCGATGGCGCTAGCTGCGTTCATATGCTTTAATAATTTTATTTAATACATGGATTACTTGACTATCAAACTGGCTTGGATTTAACCGTGCTGTAAAGTTTTTTAGCAAGCCGTTGTAGTATTTCACTAACATTTGTTTATCAGTAAAGTTAACCAAATGCGGACCCAAGATCATTTGTTCTTCAGTTAAATACTGGGTTTGCGCCTTTGTTTTATCCACAGCTAAAACTAAGTAAATAAAACCACGTTCTTCAACTAGTGTTTCAGTGGTAATAGTTCATTGATTAGCACTTAAAAAGCGGCGCAATTCAATGATGTTGGACTGCGGTTGGAGTACAAAGTGCTTTAAAAAGTTATTGTGCTGTGCTAGTATTTGCATAATTTTCAACCCACCTAAACCAGCAATAACACCAACAGCAGGATCTAAGTTTAAGTTAGGTAACAGGTCAAACCCGTCACTGGTAATAAAGTGCATGTTAGTGTGATCTTTTAACAACCCTTTAGCAGCACCTAATGCATTAGCACTAATATCACTGTTAACGCCCACTAAACTAGGGTTTTGTTGAACTAGTTCACGTGCTAAATAGCCATGGTCACAACCAATGTCATAAAAGGCTTTGGGTTTTTGCTGTAAGACTAAATTAGCAATTGTGCTAATCCTTCTTTTCATTTCCCCTCATGAAGGAAAGGCTCATGTGGTTATTACGCACTGCTTGACGCAGTTTGCGAATGGCTTTGGTTTCTATTTGCCGAATCTTTTCGCGGGGAATCTTAATCTTTTGACTTACCTCATCGAGTGTTTTGGTTTCGTTGTAAGGTGGCATACCAATGCGCATCCGCACAATTAATTCTTCCTGTTCGGACAAACAGTTGTTTAACAACTCATCAATCTTTTCGTAATTAGAACGGCTTTCGGTAAACTCATCGGGCATTTGGGCATCAGTATCACGCACAAAGTCACCGAACTGCGATTCTTCATCGTGGCCCACTGTTTTATCTAAAGAAACAGGATCTAAGCTTAAACGTTTAATTTCAGCAATCTTTTTAACTGTAAACCCTTCAGCTTGACCACCCATTTTTTCCGCTAACTCTTCCGCGGTGGGTTCGCGTCCTAATTCTTGGTTTAAGGCACGTTCAGCTTTAGCTAAACGGTTAATAGTTTCCACCATGTGTACGGGAATCCGAACCGTACGTGCTTGATCAGCAATAGCACGAGTAATCGCTTGTTTAATCCACCAAGTAGCATAAGTGGAAAACTTATTACCCAATGACCAGTTAAACTTGGAAATGGCCTTTAAAAGTCCTAGGTTACCTTCCTGGATAAGGTCATTAAAATCCAATCCTCTTTCCAAGTGTTTTTTAGCAATGGAGACCACTAACCTAAGGTTAGAGGTTACCAGTTGATTAATGGCATACTTGCGTGATTCTTCATCAGTACTGTTTAAAACTTTGGCAATTCGCTGCTCAGACTCAAAGTCCAACATCTTGGAAGAATCGAGCGAACCTAGAAAGAAACGTACGTTGTCATCAACCTTATCACGATTAGAGATGTTTTTGGTGGTTAATTCTTCAATGTCCTCGTCAATGATCGACAAGTCTTGGTTAGCACGGAACTCATGGATGTGCTCTTCCACATCATGTGGTAGTTCAATTCCCTTGTCACGCAGTTCATCCAAAACAAAGATAATTTCATCATCTGGGAGTTCAAACTTGGACAAAACATTAACAATGTCCTTGTTGGAAAGCGAGTTATTGTCTTTCTTTTTGTTCTTTTTCTCCCACACCTCCACCAACAGGCTAATTACCTTGTTAGTATCATCATTTTCGGTATGACTCTTTAGAGAGCCTTCTAAAATATCAAACAGCTTAAGGTTGTTCTTTTCCTTTAAAGGTGCGTGTTTTGGCTTTCGACCGCGACGCTTCTTTGGTACATCACTCTCCTTGTTTTCTTGAAGCGTTTTTAAAAAGGCTACGTTTTCGTGTTTTTGTTTACGTTGTGCCTTAGTGTCAACGTAAATCATCTTTTTAAACGGACGGTTTTTACCGAGGATACGACTCTTTTTAAGTTCAGCAATGCGCTCTTCGTTAAGTGCTTGTTTTTGATTTTCAGTTTTAGGCTGAGGTTTTTTGAAATTTTTCTTTGGCGATGACATTCAGTCTAGTGCGCACGCGTATACACGCACGCCAAGTGGAATTGTACATTATTTAGTGCTCAACCATGCTTTTAAAATATTTTAAAAGCTCTTTTTGTTTCACAATTAGTTGCCGAAAAAACTGTAAATCTTCCTCATAATCCTCAGGGTCATATGAGAGTGATTTGTTTAAAAAGAACTCCTTAATTTGCGCTAAAAAAGTTGTTGGAAACATTGCTTGTTGTTCCCCTAAAATACTGTTAAACACAGCCCAATCAGGCTCTTGCACCTTAGCTCAATAAAGCCGCGCTTTATCAATAAAAAGGGCAACATCAAACAACGGTGTTTGCAATGAAGCAAACAATTCATCTAAATCAGATTGCTTTAATTCGACTAAAAACTGCTTATCTAACAGTACGTAAGCGAAGATTTCAGCATGAAATACACGTTCCTTGGTCTCCTTTCAGTTGTTTTCGACTAAAAACTCCTTGGTAATGGCAACCGGCTGCGATTCATTTCTAAAACTATTATCAACCACACCAACCACTGATTCGTAAATCGGGGCTCTTTGGGTAAAAGTTGTAGTTTTATTAACTTTTACTTTGAGCTTTTGCTGCTGTAATTGTGTGAACAGTGTTTCCACATCACTGTATTGCAACAATTGCTGTAATTTCTGACACAAAAAGCGGCTAAAGCTAGTGTCAGCTGTTACTTTCTGGTTTTGTGCAATAATGTCGATAACTTTATTGACGGTAATCCGCTCATCAAAGCCCTTGGTTTTAAAGTAAGCCACTAAGTATTCCACGAGGTTTAAAACCCTGTGAGTCTTAGCTTTAACCGCATCACTACCTTCAGCTAAAAACAACTCATCCCAGTCTTTGTAAGCGGCTTGGTTTCAATCGACCACTTCCACAATAATGCCGTGTGCGTTTAACTCTTGTAATAGCTTAAAAGTAGCATCGTGACCCGCTGTATCATTATCGAGTGCCAAGACCACTGTTTCTAGCGCTTTTAGTTGCTGTTGAAAGGCAATAATGTGGCTTTCACTCAAGGCTAAACCCATTAAGCCCACTACATCACCAATCCCAGCGCTAGTAAGGGCAAAGACATCGAAGTAACCCTCAACTAAATACAGCTTTAGTGTGTTTTTATCAATCCGGTGGAAGTTAAATAACAATTCACTCTTCTTAAACCACTGGTGTTCTGCACTGTTTTTGTATTTAATCTTTTCCGTTTTTTGCAGTGCACGCCCTGAAAAACCTACTGGATTACCCTTAAGGTCATGGATCGGAATCATAATCCGGCTCTTAAAGGTTGCATAATGCGTTTGTTGGTTAAAAAATCCTAAAGCTTCTGGGAATTTTTCAGTAGCAAAACAGAGCTCTGCCTTTTCAATTGCCGGGCTAATTCAGGGATAGTTTAAGAGACTGGGTAGAAAATACTGGTCTTCTAAAGTAAACGCTAAACCCAACTGAAAGCGTTCAATTACTTGAGGACTTAACTTTCGTGTTTCAGTTAAGTAAGTCAAACCCTGCTCTTTGGGCTCTAGCTTTAACCGGGTTTGATAGTAATCGACTAGGGCACTGTGTAGATCCCAATAGCGCTTTAACTTTGGGTCAACTTCCTTAAGGTGAAAGTTAACAGCGTATTGTGAGTCAAGATTGAGAATACTAAGTGCCTTATTGAGCGCCGTTTTTCAATCTACCTGATCAATTTTTTGGATAAAAGCAATCCCATTACCGCCCACCTGACACGAGAAACACTTAAAGATGTTCTTTGCAACTGACACCGTCATCGAGGGGTTTTGGTCAGCATGAAAGGGACAGAGTGCCACAAAGTCCTTCCCCTTCTTTTTTAGCTTAATGGCATAGTGCTCCACTATGGGAGCTATTTTGATTTGCTGTTTGAGCTGATCTAGGCTAGTGGGACTGGTCATCGTGTTGGGCAAACCAAGCCTTGAGCTCCTTGATTGGGACACGCACTTGCTTGAGTGAATCCCGTTCTCTGATGGTAACAGCTTGGTCTTCTAAACTTTCAAAGTCAAAGGTAATGGCAAACTTAGTGCCAATGGCATCAGCTTTACGGTACCGTTTGCCAATACTACCGGCGCTTTCAAAGCCAATCCGTCAGTGGGTTTGACTCAGCGCTTCAAATAATGTTTGTGCTGTTTCTTTGAGTTTATTGACTAAGGGCAGCACTACAATTTGTTCTGGACATAAGTGAAAGGGTAAACGTAACACTTCGCGCATTTCCCCTTCCAACTGCTCTTGTTGATAGCTGCTAACAATCAAGGCATAAAACAAGCGCTCAATTCCCACCGAAGGCTCCACTACAGCTGGAATAAAGTGTTCGTTATTCTCTCCATCAAAGAAGTCTAAGGGCTTTTTGGCGTACTTTTGGTGTTGTTCTAAATCAAAGGTACCACGATCAGCTAAACCCCACAATTCCCTCAAGCCATGGGGAAACTGGAATAAAAAATCCACTGTTTTATTGGCATAGTGTGCTAATTCATTCTTGTCATACTCGTATTGTTTAACCAATGCTGGGTTTAATTGCAGTACCTTAAACAAAAAGTGAGCAATTGCTTGTTGTTGGCTTTCAAAAACGCTTAAACTGGCTTGAGGATTGCAGAACCACTCCATTTCAAACTGTTCAAATTCACGAACGCGAAACAAAAAGTTTCCCGGGGTCACTTCATTGCGAAAGCTCTTACCAAACTGGGCTACGCCAAACGGTAAAGGACGCTTTTTGAGTTGTAACAGCTGTTTAAAGTTAATGAAGATGCCCTGAGCTGTTTCCGGACGAAGGTAAACCAGGCGTTTTTCGCTGTTGACAACGCCAATTTCAGTTTGGAACAAGAGGTTAAAATCGCGTACTTCTGACCAATTATTGGCCTTACAGTTAGGACAGTTGACCTGCTTTGGGTTTCACTGGGTGGCGCTTTTAATTTGTTCATCCAAATGATCAACACGAAAACGCAACTTACAGCTCTTACAATCCACAAGAGCATCAGTGAAATTGGCTAAATGGCCACTAGCTTTTCAGATCTGTTCGTTGAGAATAATTGGCGTGTCAATCAACAATACATCCCGCTTATTTTTAATGAAGAAGTTGTACAGTGCCGTTTTAATTTGTTGCTTTAAAACGGCACCCAACGGACCGAAGTCCCAGCTGTTGGCAAGTCCATTGTAAAGCTCACTACTTTGAAAGACAAAGCCATAGCGCTTGAGGTATTGCACATACACTTCTTGGGAATATACTTGAGCCATAGTGTTAATTAATCTTAAAAATTAGCTTTTTCATCCAAACAAGATAATCCCCACGGCAACACTAACATTGAGTGAGTTGAGCTTGGGATTCATCGGAATTTTGACCTTTAAATCAGCGTTTTTTAACAGTAGTGCATTTAAGCCCTTATCTTCATTACCTACCAGTAAAATGCGCTTGTCAAAGTCTACTTGGCGGTAATCTTTTGGCTTTAAGCGCTCATCTAATGTTGTAGCGACCGTTCAAAAGCCGTGTTTTTGCAGGGTAGTAATGGCATAACTGAGGTTAGTAACCTGCACTAAGTTCTGGTAAAAGACAGTTCCCATACTGGTTCTAATAACGGTACTGTTAATGGGCGCCTGGTTATGCTTTTTAAAGATAATCCCATCCACTTCAGCTGCCATACAGGTGCGCAAGATGGCGCCAAAGTTATGCAGATCTTGGATCTCATCGAGCATTACCAGGGTACTGGTGGACTTGCTGTCTGTAATTTGGATTAACTGCTCAATTGTTTTGAGCATTTGGTTAGGGTTGATTACACAAACAAATTCCTGGTGGTTAATGTGGCGAAACTGCTGGTGAAACCACTGGCTGGAGTGCATTTCATACCGCAATCCCCGTTTCTTAATTAAGCCCAACAGCTTTTGGTGCTTATCAGATAAATTGACCTTTTTAATTTGCAGTTGGTTATCTAACGCTTCCAAAAAGGCCTTACTACCAAATAAAAAACTGGTTTCTTGCATCCGCTTCATTACTATAACAAACCTTTTTTAATTAGCTTTTGGCGCACTTCATCAGCCTTGTCTAACTGACCATTTTTGCGCAAACTAGCTCACTTTTTAATTAACTGTACATTGAGCTTAGTGTGGATCGACTTAAAGCCAATGCCCAAGATGTTTAAAGCTCACTCCAAATGGCTTAAATGTTCCTTTAAACCGCTCAAGTTTTGCTTGCTTACATCATGGTTAATTTGTTTGACTAACTTCCAAATGTGGGTAATGGCATTAGCAAAGTTGAGGTTATTGAGAAGTGCCTTAAACACCGGTTCAAACTGTTTGGGCGCGGGAATGGCGGATTGTTCAGAATACACAAACCAAGTGCGGCACACATTGACTGCCTTTTGGATCCTTTTAATATCACTGCAAGCTTGCTCTATTAAAGATTGGCTTAAATCCAACGGGTGGTAATAGTGTTTTTGATAGAACAATCAGCGCAAAATGCGAAAGTCATGGATTGTCAAAAAGTCAACTGCTAAAAGAAAGTTTTGCAGTGACTTGGACATCTTTTGGTTTTCAAACATTAAGTGGCCAATGTGCATCCAATGCTGCGTTAATGGTTTGTCATACAGTGCCATATGCATTGCGTTTTCGTTTTCATGATGGGGAAACTTCAAATCCACCCCACCACCATGAATTGTCAACTGGTCTTTAAAGCTGTAATCAATTAAAAAGGCACACTCCACGTGTCAACCCGGCCGACCCCAACCTCACGGACTGTTTCACTTTACCCCAGCGGTCGTTTGTTTTCACAAAACAAAGTCCAGTTTATTGCGTTTGTCAGTAGCTTCATCCTCAATTGTTTCCACAACACGGTTGGCTAAAATACCATACTGGGGTAATTGGCTTACACTAAAGTACGTGTTGCCCAAAGGCGAAACGTAAGCAAAGCCCATTTGGACTAACCGGGCAATGTAACCGGGCATTTTGTCAATTTTATCGGTGACTTGGAGGTGTTTAATCGGCAAAATGTTGAGCTTTTTCAACAAGCTTTTGTAAGCGGTGATTTGCTGTTTGCACAACTGCGCTTCACTAATTTCCTGTTGCTGCGCGATCTTGATAATCTTGTCGTCAATATCAGTGATGTTTTGGACAAACTGCACCTTGTACTGCGCTGCTTGCAACACCCTTTGTAGCACATCAAACACAATTAAGGGACGGGTATTGCCTAGGTGCAAATCATTGTAAACAGTCGGTCCACACAGATAAATGTTAATTTGCTTTTGTTCTAATACCACGCTCTGATTGCTCACCGTGTCAATGAGGGTAAATTTAGGTTCAAATTGGTTCATTAATAATGGGAATATTGAGGGCTTTAGCTTGTTCAAGTTTACGAGGTGTAGCCTTAATGCCAGCTAACACAAAATCAACTTTACTAGTGACGTTATTTGTAAAACGACAATCATACTTATTGGCGAGCAAGTTTTTAATGGTATCGCGACTAATTGGAAAGCTGCCAGTAATAACAAAACTCTTTTGAAAGTAAATGCTCGCGGTGTCAAAGTTAGTGTTGCTCTTTAAAGCATCCATTTGCACTCCAACTGCTTTAAGTGCAGCTAACAGCTGTAAGTTATTGTCATTGTGAAACCAGTTAAACAACGATTCTGCCACAGTCGGTCCCACATCAGGGAGTTCTAACAGTGTTTCCATACTGGCGGATTGTAAGGCATCGATGTTTTCAAAGTGCTTCGTTAAGCTTTTGGCTAACACTTGCCCCACATGTTTAATGCCAAGCCCAGTTAACAAGCGTGCCATCCCCTTGGTTTTGGACTGTTCGATGCTGCTGATTAGTTTATTGAACAGCACTTCACCAATTTTAAGTTCCAAGGCTAACACCGCGGCTTTGTGCTTCTTTAAGTGGTATAAATCAACGACGCTGCGCACTAAGTTATTTTCATACAGCTTACTAATGGTCTGGATACTTAGCCCCTGGATGTCCATAGCCGTTTTGGACACAAAGTAATTAATCGCTTCAAGGTTACGCTGGTCACAACGTTCATTGGTACAGTACTGATCTACCTCATCCTTATACTGCACTAACGGGGCTTGACATGCCGGACAGTGGGTTTGTTCGGGAATCGGTTGATTCGCTGCTGTTCTTAACGGTAAACACACCTCCAATACCTTAGGGATAATTTCCCCCGCTTTGTAAATTATCACTGTATCGTTTAAACGAATGTCCTTCTCCTTAATGTAGTCAAAGTTATGCAGTGTGGCTGCTGATACTACTGTCCCCTCTAAGTTAACTGGATCTAACTGCGCGGTATAGTTAATCTTGCCAGTGCGTCCCACTGTTACCAAGACACCACGCAGTTTGGTTTGGACAAACTTCGGTTTAAACTTGTACGCCAACGCCCAACGGGGAAACTTACTAGTAAACCCTAACTGGTCGTAAAAGTGAAAGTTATCTAGTTTAACCACTAGGCCATCGACTTGGAAGTTCAACTGATCACGTGTTTGGTCAAACTGATCGAGGTACTCAATTAATGCTTCGGGTCGATCAAAGCTAGCCACCGCTTTGGACACTGGAAAGTTTCACTGCTTTAACTGTGCTAACACGGCACTTTGACTCCTACAACCCAAAGTTAAGGCGTTGGGAACAAAGTAAAAGAAGGCCTTAAGTTTGCGCTTGGCACTCACCTCACTGTTGAGGTTCCGTAGTGTACCTGCTGCTAAATTGCGGGCATTGGCAAACGGTTTTGCTAAATTCTCGTTAATTTGCGCAAATGTTTTTTTATCGACAAAGATCTCACCGCGGATTTCCACCTGTTTGTGAAAAGGAATCGTTAGGGGAATGCTCTTAATCGTTTTAACATTACTCAAAACATCCTCACCCACAAGTCCATCACCGCGGGTTAAAGCCCGAGTTAACAATCCGTTTTCGTAAGTTAAAGAGATGCTTACACCATCAATTTTGGGTTCCACCACAAAGCGGTTTTGCACTCCAGTTAAGTTGTTAATGTTGGCAATAAAACTAGCAATTGTTGCTGTGTCGAAGGCATTGTCTAGGGAAAGCATTGGATTTTGGTGAGGAAACTTATTAAATGCTTCCACTACAATTCCCCCAACACGTTGGGTTGGTGAATCGGGTTGGATTAACTCAGGATGGGCTTGCTCCAACGCTTGCAGCTGTTTGTATAACTGGTCGTATTCAAAGTCACTGACCAGCGGATCGTCCAAAACATAGTAATGGTAATCATAGCGTTTTAACTGTTCAACTATTGCTCGAATTTGTGCGACCTTTGCCATAAATTAATAAAAAGCCCTGATTAGCTTACAATGCCAACCAGGTGCTTTTAGGAAAATTTAATATGGAGCAGATAACGGGAATCGAACCCGCATCTTAACCTTGGCAAGGTTATGTTCTGCCATTGAACTATATCTGCGCAGCTCATAATTTTAGTATATTAAAGCTAATGGAAACGCAAAACCAAATAGAAACGTTGCGCTATATTTTTAATCAGCTCAACAACCAAGACAAACCACAAATAATTTGGTTTTCCGGTGAAGGTGAGGATGAAAAAATTAACTTTTTAATCCGTTTGGACAATTATTTCCAACCTACCTTTGTCCAAGACCTTACTATTAACTTTCTCCCCGCTTTTGTTAAGCGAAACAAGAAAAATCCACCCAATACTTTAGCTAAGGGTAACTTTGTCAACATAGCTAATAAGTTATTGGCAGTGTTAGCGCGTTCCTTGAGCTGAAAGCAGTTAAACAAACCCCAGCAAAAATGGTTGTTATGGTTACTAGTGCCCTTTTTGCTTTTGCGCCAACTATGGTTAAAAAAGAAGGTAAGCAAGATCTTTCAGTTTGTCAATGAACGTGGCATCTTGAGCTTCATTAAGGAGCAGTGACCGATTTTAACGACACTCGTTACCGTGGGTACTACCTTAGGTACACCCATCTTTTCGATTACCATTTCCCAGCAAAAAGCAATTTTGGAAAACGCTGGTCATGGTGCGTTTGTCTTTTTAGTAATCTTTTCGGTGTTTGCAATTGCGCTGGGTTTAGTCTCATCGTTAATCTTTTTAGTCTCATCGTTATTTTCAATTCGTCAAAAAAAGAGCTTACAACAGCTCCACCAAATTCTCTCTCGGTTAATTAACAAGTACTTCTGTTTGGCTAACAGTGAACAAAATCAAACCGGGCGGTACCAACTGAAAAATACCGGGGTGTGCTTCTTTTATGGCTTTGACTTTGAGGAAAAGGAATACATAACCCAAGCGATGAACCTACTGTTGCTCTTGAAGCAAACGAACTGCTTTGTGTTAGTCGGTTGTAAAGAGAGCAACATGTTGTTAATTAAAAACAAGGTGGAACCCGACATTAACCTTAAACAAAGTAGTCTGTACCTTGATTTAAAGAGTCAAATTAGTCCGTTGGCACAAATTAGTAAGTACAACCTCTTGTTTGAAGAGTTGGCCCTGGATGCTGACATGTTTTACTTAGAGGACTTCTTCGCCTTGTTAAAGACTCCGCGGCAAATTGTCAACTTTTTGTTTCGTATAAAACAAAACTTAAAGGAGTTTCACCAACCCCAAACGTTGTGGTTTGACTATTTGGCCTTGTGAGCCTTAGTAATTGCGACTGACTTTGAATTTAACAATGTCTTGTGGTCATTTAACGACTATTTAAGTTTAACGAACAAGCAAAAGGAAGATTATGCATCAGTTAACCTCACCGCCTTTTTTAACCGTAGTTTAAAGAACCATAAGGACAACAGCCTGCTCTTTAAACCAGAATTGTTCAACACTCACGCTTATATTCCTGAAACTTACACTCAAGTTACCTTAGAAAACATTGATTCAGACAAACGTGCGCAACTAGTGGCATTGAACTGGTTTTCACAGCAAAAGTTTAGCGATTTTATAGAAGAGAAAATTAACTTTTGACAAACTCAGCAGGCGGAAAACAAGGTGTTTTACCTCACTTTGGGCGAGCGGATCTTCTTTTTAGTATTAGTTAACAAGAAGTTCAAACAAATTAAACTAGAAGCAGCGTTGAAGTACCTCAACTAACTTCCCTTGGTGTAATGGATAACACGACTGAGTCCTAATCAGTAAATGGAGGTTCGATTCCTCTAGGGAAGGCCAAATTATCACAGAGACCTAGTTAACTACTAGGTCTTTTGTGTATATAAATTAAATTAAAATGATTAACCGCTAAGGGGGTGTAGTTTAGTGGCAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCACCTAGTGATGCATTACAGTTACAGCTTTAAAGAATACATCGAACGGTTTGCCAAAAAGGTTAATTCGATTGACAGCGAGTACTTCGAATTTTCCTCTTACATAGAGCGCATGCGCACTGTGTTTGGTTTACTGATTGCACTGATATGCTTTAGTAACGTTCTCTGTTTTCTTTTTATTGCAACTTGGTTTAGCACAAAGGGATTTGGTCAACACTACCGGGCGTTAATATTTACCTTGTTTATTCCCTTTGTCACTTCACTTTTAGCCAACATTATCTTCATTAACCTTAATCGCGCTTTTCGCGAATACTTTAAGATCAGCAGTAAAAGCCGTTCTTTTTTGGTAATCTGTGCCTTCTCCTCATTACCGATTGTGAACATCTGGCTAATGTTGTGGTGAGTAGCGATGATTAAGCGCTTTACTAGTAACTATGCCTTTGCTATCTTTGATAAGTACAACGGTTTGACTAGTGGTGTGTTTATCTTTGACTTTGCTGATAATGTCAATTTTGAGGGTAAACTAGTGAGCTTTGACAATACCAAGGACACCAACCGTGACTTTGTCCACTTTTACAGTGAGGCCAAACTTAAACGCGATAAAATTACGCTACAAACGAATCCAATTCCACACGAAAGGATGTATGTTAACCGCATGTACTATCAGCAAAAGCTCAGTATGGGTGCTAACCAAAACAGCCCTTCAACGGCATTTGCTAACTTAAAGCGGTATGTGGAACATAAACAGCAAAAAATTATCAAGATTAAGCAGTTTATCTTGACCTAAAAAAGCAATAAATATATAATCTAAGCCGTTGCTTTGTAAAACCAATGAAAAAAGATTTTCATTTTCCAAGCCAATCCGTTAGCTTCAAGTGTGCTTCTTGCAGCAATAGTTTCACCATTGAATCAACCCTAAAACAAAAAGAAATTACGATTGATATTTGTGGTAAATGTCACCCGTTTTACATTGGTGAATTAACCAAACAAACAGTCCACGGTCGTGCGGAAAAGCTTTCTGGAAAGTTCAATGCTGGCAAAGCCTTTTTAGAGAATAAAACTCCTAAAAAAGCGAAAGGCAAAACAGAAGAATATACCAAACACCGTTCTTTAAACGAACTGTAGGTGGAGTTTGACAAGCAGTTCTTTTCTAGTGTTGAAAAGATTGTTGAACTTGCTGAACAACTCGAAAAGGACTTAAATAAACCCGATTTGACCTTTGAACAGATCAAAGCGATCAACAAGGAACTGAAACATAAACAACCCTTAGTTGTTAAATTCAAAGAATTTAAACGGCTCATAGATCAAGCTTTGGAAGCGGAGGCCATTTTGGAAAACAACGAGCTCAAGGAGCTACATGATGAGGCCAAAAAAGAGCTGGAACGGGTGCGCAGTGTTGTCCCTGAATACGAAGAAGCGTTAAAACTGTTGTTATTACCAATTGATGAGAATAACCAGAAAAACGTGATTGTAGAGCTTCGTCCCGCTGCTGGTGGGGATGAATCGTGCATCTTTTTAGCTGATTTATTCAATATGTACCGCAACTTTTGTACTAACAAGGGTTGAAAGTTGCAAATTAACGAAATGATCCCATCCAGTGTCGGGTTAAACTTCGTTTCCTTTGAAGTTAATGGTGTCGATGTGTTTGCCAAGCTCAAGTTTGAATCTGGCGTACACCGGGTACAACGCGTACCCGCTACTGAATCCAAGGGGAGAGTACACACTTCAACCGTTACGGTTGCTGTTTTACCACAACTAGAAGCGGTTGAAGTTCATATTAACCCAGCTGATCTTAGAGTAGATACCTACCGTGCTTCGGGTGCTGGGGGTCAACACGTTAACCGGACTGAAAGTGCAGTGCGGATTACCCATTTACCAACTGGCATAGTAGTTTCGTGTCAAGAGGGTAAATCACAATTTACTAACCGTGATACGGCAATGAAGATGCTACGTGCTAAACTGTGGGAAAAAGCGCAGAACGAACAGTTATCAACCCAAGCAGGTTTGCGTAAAAGTCAGGTTGGGTCGGGTGATCGTGCCGAAAAGATCCGCACATATAACTATCCCCAAAACCGGGTCACAGACCACCGCATTAAACTTACCGTCAATAAGCTTAACACGATTATTTTAGGTGACCTTGATGAAATTATTGAAGCGTTGCAGGCAGATGAAAAGAAACAACAACTAGAGAACTTTTTCAGTTAATGAACTTGTATGAACTTTTCTTGAACCAAAAGCTGCTGTATGGCACCGATCCGCACTTTAACGGTGTTTTTCTCACCCTATTAGAAAAGTTTGGTCTGCACTTTAAGGATTTAACAGCACTGTGAAAGCACGCTAAAACAATTACTGACTTTGACGAACAGGGAATTGTTAATGCTTTAAAAGCTTACTTTGTCGATCAACTACCCTTACCCTATATTACCGGGAGTGTTAAGCTAGGTTCATTAACTTTTAAAACTCAACCTGGCGTCTTTATTCCTCGTGCCGACAGTTTAGCGCTTTTAAAGGTAGTCAAAGCACAAAACCTTAAAACCGCGGTTGACCTTTGCTGTGGAAGCGGTACGTTAGCAATTGCGCTAAAAAAGCGTTTTCCTCACTTAAATGTTTATGGTAGTGACCTCAACCCCCAAGCATTGCAGTTAGCGGCGCAAAATGCCCGTTTGAATATGGTTGAAGTGCAGTGAATTGAAGCTGATTTTTTAGCTGCTCTAGCACAGGTAAACACACCTATTGATCTCATTATTACCAATCCACCCTATTTAAACGAAAGTCAGTTAGATCAAACTTTAAACCACGAACCGCGCAACAGTTTAGTGGCTGATGGTAACGGAATTTTGTTTTACCAAAAGCTGTATAACTTTTTATTGGGTAACCGCCAAGTTAAACAAGTGATACTGGAGTGCTCACCAACCCAGAAAAAAGAGTTTTTAGCATTATTTAGCATTTTTAAAACTAGTGAAATTTACACAAGCCACAAGCAATTCATCGGTTTGAGTATTGACAACACTAAACTACCAGTCTTAAAAATTGCGCAAACCAAGCAAATTAAAGCACTGCTAGACAAGGGGATGACGGCCATTATCCCCACCGACACGCAAATAGGATTAATGAGTTATTGCCAACAAGATTTAGACCACATTAAACAACGCGATCCCAACAAGCACTACGTCCAGTTTTTAGCACCTAGTCAAATTAACCAGTTACCCAAACAATTACAAAAGCTAGCCAAACTGTTTTGACCAGGTGCTTACACCTTTATTGTAGATGGACAGAGCTATCGTCTACCCAACAGCCCACAGCTGTTAAAGCTCTTAAAAACGGTTGGTTTAATTTACTGTACCAGTGCTAATCAAGCTAAACAAAAACCATTTGGCAAGTTAAGCGCTTACCAGAACGATCCTTACTGAGTGCAGCAGAACTGTTTTATTGTGCAAAACAGCTTTAAAAGTAATAACGAACCGTCCTTAATTTACAACTTGGATACGAAGCAAATTGTTCGTGGTAGTTCAACCCAATTACAACGTTTCCAAGCGTTACTAGCAAAACACAAATTAAGACACTAAAAGTTTCAAATTTGACTGCTAAAAAGCACTATTAACGGATTATTGGAGCTATCCAACTTATTTTTAACTTTTAAAAATAAAGAATTTGCACCATTGCTGTATAATTTCCCATCTTTTTGGTGTTTTGTAAGATGAAATTTAAGTATGGTGCTACCCTTTTTAGTGGCTTTTTAGGGCTTTCAGCGATCCTAGCTGCTTGTGGTGCAAAGGGTAAATTTGACCAAGTTGATGATGGCAAAATTGTGTTAGCCTCTTCATTAACTTCTAAAAATGCCGCTAATGCTCTACAAGCTGTGGTGGAAAAATACAACCAGGTTAAGGGTGGCAATGATTATCCTATTGAAATTACCCAAATTACCGGTGGTTATGACGGTGGACGTGGTAACTTACAAACCAAACTTAGCGTTAAAGACAAAACGACCTTTTACAACCTCATCTTAAACTATCCCGACTTAGTTTCCACCCTAGGACGTGTTGGGATGGAACTGCCACTAGACAACGTTGATCTCTCCAAGTTATCACCTAACTTTTTGAAGTTCAACGAACGCATTAGCGGTGTAGCCAAAAAGGCCAATTACGCCATTCCCGTATCACTTTCAACTGAAGTTTTAGCTTTAAATGGACCGGTATTACACTATATCTTGAACAGCGCGAAGGGTGGTAATGAAACAGCTGTAACACAAAGAACTGCTAGCACAAACCAACAAAATACACTTCAAAATTCGTTAAAAATTAACGAAAGCGACCCCATAACCAAAGAGCTTTGAGAAAAAATTCAAAAGGCTGCTGAAACTAATGGAAAGGCTAAAGAACAAGGTAAAAGTAAGAAACAAGGCACAGAGAAAACTACCACTCTAACATTACTTAAAACTGAATCTAGTCAACAATCTAACACCACTAAAGATGGTAAAGCTACAAGCGATGAAGCAATTAAGAAAACTTGAGGTGATTACCAAAAGGTGGAGAATGGCCTTAAGGGTTATACCTTTAAAGCTAGTGTCTTTGAAAACTGGCATGAACTGCTCGATTTTTCCGCAAGGATAGCTAGATCATTTACTAATATTCACACCAAAGAAACCAAAAAGGGAACGGACATTCAAGGTGTACTTGGGATTGACAGTACACCTAACGCCTTATTTACTTCAGTATTTGCTGCAGGTGGTGGTAACTATGACAACTTCTTTTATAAGGTGCAAGATGGTCGGGCTGACTTTAGTAACTTTAAAAATAAGGAAACCTCTTACCAAAACTTACAAAAAGTATTTAGCGATTATAAGGAGTTAATTGATAAAAACGGTTTGTTTGTCAATAAGGGTGGCTCTTACTCTTCTAACTTCCAGAAGTTCCACCAATTAGCTTACAGTATTTCTTCCACTTCAGGTTTCTTTTATTCGTTTGCTGGTAACAGTGCTAAACGCTTAAAGTTTGATGACGGTAACTTTATTGAGTTTCCGCGCTTCACTCAACCAGTTAAAGCGCCTAATAAAACAACTCAAAGTGGTGGAGAAAATAACAATAAAAGTGAAAATAATCTTCTTGGAACTTTTGAAATAGAAGACAAAAGCAAAAAGCGCGAAAGCGTTGAATCTAGTGAAAAATCAAGTTCTGGGACTCAATCCAAAAGCAAGAAAGGTAAAAGTATGAAAACCATTTACCTTTATAAAGCACAAATTCCAGAAGGAAAAAACAAAGGTGATAATGCCATCTTAATTACTAACCAAACTGTTATTGATAAACTAGAAAAAGCTGCTAAAGAAGACGGTAAAAGTGGAACTCAAGTTTCAACTGTTGCAAGTAAATCAACAACTAAAGAGAACCAAGAAAACAAAATAATTGGTTACACATCGACTGATAACCTTCGTGAAGACAATAAGAACATTTTCGATATCGATGATCTTAACGATGAAAAATATGACCGTAAGATTATTGTTAGTGCTACTGAAGAAGTATTGGAACAATCTAACACTTTACAGAGTGAAGAAGCAGTTGTTCTACCGGCACTTGCTAAATATAAAGCTAGTGATTCCACAAAAATAGCAATCACTCAATAACCTAACCTAATTGGTATCCATGCTAACGAAAAGGAAAATGAGGAAACACGTAAGTTTGTTAATTGGTTTTTAAACAGTGAGCAAACTTGGGAATCCAAGAAATACAGTAAAAATAAGCAAAACTCCAACGAAAAGAGAACTCCTGCACAATTCTTTGCTGATTCCGCGTCTTACATTCTACCCTTAAAGAAAACCTTCGATAATGCCAACAAAAAAGAAGATCAAAGTAAGGCCAATAACAAGACTAATAGCCAAAGAACTAACACCTATATCGAAAAGGCATTAGAAATTTTTAAAGGAATTAGCCAAAACCAAATAACAAGTTACAGTGATCCAAGCGACTTTAGAAGTGGTCGCTTCCGCGATGGTATTGGTTCTAACTTTAATGCAGTAGTAAATTCCAAAGTGGATTTCAATAAATTTATTAATAACTTTATTGCTAGTCTAGGTTTTGACATCTAATAAAGCTCTTGTTGAAACAAATTAAACTGTTACTGTCGTGGAGATTAAAACTTACAAAATCAAAGATATTTGCGAGATTTCTAGGGGCAAAGCAATTACCAAAGAATACATAAGAGCTAACCCTGGTGAATATCCTGTTTATTCTGGTTCAACATTAAATGATGGTGAAATAGGTCGCATAGATGAATGCGAATTTGATGGTGAATATGTAACTTGAACAATTGATGGTTATGCTGGCATAGTTTTTTACCGCAATGAGAGGTTTAATGCCTCTCAACATTGTGGTGTCTTAAAGGTAAAAAGCAATGAAATTTGCCCTAAATTTTTGGCATATGCATTGGGGATGGAAGCGCCTAAACATGTTAATAATGCTTGTGTTATTCCTAATCTAACTTTAAAAAAGATGAGAGAAATAGAACTAGATTTTCCAAGCAAGAAAATTCAAGAGAAAATTGCTACTATCTTAGATACTTTTACCGAGCTAAGCGCCGAGCTAAGAGAGCGAAAGAAGCAGTATGCCTTCTATCGCGATTATCTGCTCAACCAAGAAAATATCCGAAAAATTTACGGAGCAAATATACCTTTCGAAACTTTTCAAGTAAAGGATATTTGTGAAATTAGAAGAGGTCGAGCGATCACTAAAGCGTATATTAGGAATAATCCTGGAGAAAATCCAGTTTATTCCGCAGCTACCACAAATGATGGTGAGTTAGGACGTATTAAAGATTGCGATTTTGATGGTGAATACATCACTTGAACTACAAATGATTATGCTGGAGTAGTTTTTTATCGTAATGGCAAGTTTAATGCATCACAAGATTGCGGTGTTTTAAAGTTTAAAAATAAAAAAATCTGCACCAAATTCCTATCCTTTTTATTAAAGATAGAAGCACCAAAATTCGTTCATAATCTAGCTTCAAGACCAAAACTAAGCCAAAAAGTAATGGCTGAAATCGAACTCTCTTTTCCACCTCTAGAAATTTAAGAAAAGATTGCTGATATTCTCTTTGCCTTTGAAAAACTTTGCAATGATTTAGTAGAAGGTATTCCTGCTGAAGTCGAAATGCGTAAAAAGCAATTGGATTACTATCAGAACTTCTTATTTAATTGAGTGCAAGAACAAAAAACTCAATTAGAGCAAATTATGTAATGCTTAATAAATAGTTTCCAACACCTACCTCCTCCAAGACCACAACACCCTCACCCCCTACACGCCCTTTACCACACCGCTCAATGGGGGGCTGGATGTCGTGCGGGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTAGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAATTCCAGGACACAACGAGTTCGGACTCTAATAACCAAGATACGAGTCAAAATGCCTTGAGTTTTGATACCCAAGAATCACAGAAGGCACTTAATGGCTCGCAGAGTGGATCTTCTGACACTTCCGGGTCTAACTCCCAAGACTTAGCCAGCTACATCCTCATCTTCCAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTATACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAACAACCCCGCTCCCAAGGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCGGAAGCTGAATGAAAGTTGACCGGTGTATGAACCCCTGGATTCGACCAAGGAGGGGAAGGGGAAGGATGAGAGCTCTTGGAAAAATTCGGAAAAAACAACGGCGGAAAATGATGCCCCGTTGGTGGGGATGGTTGGAAGTGGTGCGGCTGGAAGTGCTTCTAGTTTACAAGGCAATGGCTCGAACAGTTCGGGGTTAAAATCGCTCTTGAGATCAGCACCTGTCAGTGTTCCACCAAGCAGTACAAGTAATCAAACTTTAAGCTTATCTAACCCCGCTCCTGTGGGCCCACAAGCGGTTGTAAGCCAACCCGCGGGGGGTGCTACGGCAGCAGTGTCCGTCAATCGCACAGCGAGTGACACCGCCACCTTTAGCAAGTACCTCAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATTGTTCCGGGGTTGGAAAAATGAGGTGGTAACAACGGTACGGGTGTAGTGGCTAGCCGACGGGATGCTACTTCCACTAACCTGCCCCATGCGGCAGGTGCTTCCCAAACGGGTTTGGGAACTGGTTCGCGGGGCGAACCAGCTTTAATCGCAACGTCACAGCGTGCCGTCACGGTGGTTGCTGGCCCCCTTCGTGCGGGCAATAGCAGTGAAACTGATGCCCTACCGAATGTCATCACCCAGCTCTATCATACTTCAACCGCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGAGTTCCGACCGGGTACCGAGTCTTTGGTATTGAGTTGTCGGGGAGGACCAGGAATCGGGCAAAGCGACCTGATGAGCGAAAACCGAGCTCAACTGGGGCACCGACAAGCAGAAGCAGTTTGTCGAAAACCAGTTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCATTTAAAGCGGGCGCGGTGGGGTATGATATGACGACTAATTCGAACGCTTCGACCTACAACCAAGCACTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGATGGGGGGACAAGGCTTTGGTGGAAAACACGGCCGGGCTCAACGGCCCGATTAATGGTTTGTTTACCCTGCTCGACACCTTTGCGTATGTGACCCCCGTGAGTGGGATGAAAGGGGGGAGTCAGAATAATGAAGAAGTGCAAACGACTTACCCGGTCAAGTCCGACCAAAAGGCCACCGCCAAAATTGCCTCCTTAATTAATGCCAGCCCACTCAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTTCAACAAAAGTCACTCTATCTGCTCCCACTAAATAAATTGACAGGGTTTTATAAAAAAGCCAAAGAGTTTGAATATTTTTGTAATTTGGGTTTTTAATACCTTCTAACTATTAATAGGAACTTTTCTTATTAATAAGAACTTTTTTGAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAGCACAAAAACCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCACTGCTGCAAGTAGTAGTTTTCTCAACCTCTTTCAGAAAGGAGTCGCTTATTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATTACATGATGAAAAAGACCAAAAAAGGCACTTTTTCGGGATGATACATCATTAATCCAGAAAACAACAGTGTGGAATTCTCTGGCAGTTTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACAGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTACAAAGATTGAAAATAAGATCGATAAGCTTGAAATTAAAGTTGATAAGCTAAAGAAGAAAGTCGATAAGCTGGAAGTTAAGGTTGATAAACTAGTCGAAACTGTTAATGCTCAAGGAGAAGATCTTAACAACTTCAAAGTTGAAGTGAGAGGAACACTTCAAAGCCAAGGCGAAACCCTGCAATTAATTTTGCAAACGCTACAAGACATGAGTAAGCGGCTTGATTCTGTTGAAGGACGTTTAGACTCTATGGATGGTCGTCTCGATTCAATGGAAACTCGTTTGGACAAAATCGATCCGCTAAAATAGTCTTAGGCTAATAACAAAAGTGCCAGCAATTAATCTATAAAGGTTAGTCGCTGGTATTTTCATTTATTTGAAATAAATAAAAAAGCCATCCTATCAAGGAGAAAGACAGACAGCTAATGAATTGAAGTCAATTACTTAATTAAATATTCCAAGTAAATGAAGAGATTAATATCTGATGAATAGCCTTCTGTATTATTAGCAAAGGTACTTAAAAATTTGTTCACACTTTTTTCACTAACATCATCTTTGCCTTTTAATGAAAAGCTAAGTGAAGCGCTAAACTCAATTCACTCTAAATTTCTTGTAATTTCTTTTCCATTAACTTTAATTTTTTCTTCTTTAGTAAGAAATTTTTCTTTAGTAAATTGTTTTCCTGGATCAGAATTAGATTTAATTTTAAGGAAAAGATCAAAATAATGCTTGTCACCTTTCTTAGCAGTGAAATCACCTGAAGAATAATCTCCAAACCAATTAAAGGTATAGCTCAACTTAGCTTCAATTGACAAATCCTTAAAACTTTGGTATGTCATCTGGAAATATTCAATCTTTCGCTCCATCGTAAAAGGATCCTTGCTTGCGGGATAAATTACATCGTCAAAATCAAAATCTGGAATACCTAATTCGCTCTTTTCCGCACTTTGTTTTCAGGCGTCTAACAACAATTTTGTTGTATCTTTCGGATTAACTTTGTAAGACTTCTTTAGAGAAGTTATCATGTTCTTTTTGCCTTCTGTAGTTTTAATTGCTTTATACACATCAATGTCTTTACCGGTGGCTTCTTTAATAAGATTTCTAAGATCTGCTTGGATACCAGTACAGGCGCTTAAAACTGTTCCACATAACAAAAATAAAGAACCAAAGGAAACCTTTGACAAAAACTTCTTTTTGCGCATCAACAGTAAAAATAACTCTTTAATTTTGCTTTTTTTTTTTTTTTGTAAAAAGCCTTTTTGGGATAAGAACTTGAATTGGAATTTCGGGTTGAACATTAATTAAAACACTGAAATTTCCAATCATTTATTAACAACTATCTACTGATAAAGGTTTTGATCTGAGTAGTTCTAATAATTGCTAATTGTTGGCATTTTTATTCATTTAATGCTTTCTAAAAACTTTTTGCAATTTTGTTGGCACTTCTCAATAAGTCGGTCAATTTATTTAGTGGAAGAGCAAATGCAAATCTTTAATTTTTTGAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAAAAGAAAGGAACATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCAATGAAAAACTTTAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGACGGTACTTGTGAATTCATTATGAAAATTAAAACTGTAAATGAAGTTGCTGACACTGAGATAACAAATAAGGAAAAGCCTTTAGGCACTTGAAAGAGTTAGTTAACTTTTTGGTCTAAAAAGACATAAATTTACCAACGGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTTCCCCCACGCTCACCAGGTTTAGTACCGGGTTCAACCTCGCGGTGTCGGTGCTCGACCAGGTGTTGGATTATGTGCCCTGGATTGGGAATGGGCACAGGTATGGGAATAACCACCGGGGCGTGAATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTGGGTGGCAGTCAGACGACGACTACGGGGAAAGATATTCCCAAATGACCCACCCTCGACCCAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGGCTTCAAACAAACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGCAGTGGCCAGCAGGGTGGTTCTACAACGGGTGGTTCAGCGGGCAATCCCGGCTCGTTAAAGCAGGATAAGATTAGTAAATCAGGTCAGAACTTAACCACGCAGGACGGCGCGCCCCAGTCTAATTCGACGACGGAATCCGCGTCGAATTATGATCACCTCCCCCCAACCTCACCCCCACATCCGATTTACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTCTTCCTCCGCGGCTTGTTGGGCAGCATCCCGGTGTTGGTGAATCGAAGTGGGTCAGATGATTCCAACAAATTCCAAGCCACCGACCAAAAATGGTCTTACACCGACTTAAAGTCGGACCAAACCAAGCTCAACCTCCCCGCTTATGGTGAAGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGACGACGCGAGCGGGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCGCAAGACGTTGGTAACCTCGTTGTCAGTGGTACCAGCTTGAGCTTCCAGTTGGGCGGGTGGCTGGTCACCTTCACGGACTTTGTCAAACCCCGCGCGGGTTACCTCGGGCTCCAGTTAACGGGCTTGGATGCAAGTGATGCGACGCAGCGCGCCCTCATTTGGGCCAAGCAGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGCTTGGGCCGGGTCGAGAGTGTGTGGGATTTAAAGGGGGTGTGACAAGATCAAGCTCAGGCGGCCGCGCAAGCAGCTACCACCACCGCCGCAACAGGGGACGCCTTACCGGAGCACCCCAATGCCCTCGCGTACCAAATTAGCTCCACCGACAAGGATTCGTACAAGGCTTCCACTCAAAGCTCCGGTCAAACCAATTCCCAAAACACCTCCCCCTACCTCCATTTGATTAAACCTAAGAAAGTCGAAAACACGACCCAACTCGACCAGGGCTTAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAAGCCAAACCCCAATCCCTCAAAAAAACCACACCGGTGTTTGGGACGAATAGTGGTAACATTGGCAGTGTGCTTAGTGGTGGGGGTGCTGGAGGAGCAGACAGCACCAATTCGGTGGACCTCTCCCCCGTTGAACGGGTGAGTGGGTGGCTTGTGGGGCAATTACCCAGTGGGGGTGGGGGGAATAGTAGTGAGGATATTAAAAGTGTGCAAGACACTCCTATTTATGATCTATATTCATTAGTATATTCTTTTTAAATTGTTCCCTCACTTTATTCATATGAACCACCGCCTCCCTCGCGACGGGACTCACCGTAGTGGGACACTTCACAAGTACCACCACGACGCTCAAGCGCCAGCAATTTAGCTACACCCGCCCTGACGAGGTCGCGCTGCGCCACACCAATGCCATCAACCCGCGCTTAACCCCGTGAACGTATCGTAACACGAGCTTTTCGTCCCTCCCCCTCACGGGTGAAAACCCCGGGGCGTGGGCCTTAGTGCGCGACAACACCGCCAAGGGCATCACTGCCGGCAGTGGCAGTCAACAAACCACGTATGATCCCACCCGAACCGAAGCGGCTTTGACCACCGCCACCACCTTTGCGTTACGCCGATACGACCTCGCCGGGCGTTGTACGACCTCGACTTTTCGAAGTTAAACCCGCAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGTTTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAAAACAAGGTCCCCGTCGAGGTGGCGCAAGACCCCTCCAATCCCTACCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGGTTGGGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCCACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCGCTGAGGCCACTGGTTCTTCAACCACATCTGGATCTGGCCAATCCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGAACGCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCGCGCAACCGTACCGTCATTGACCGCGTTGATCACTTGGATCCCAAGGTGATGGTGGATAATTACTGTTAACAATTTTTTTAGAAAAAGGCAGTTTTTGAAGTTTTTAATTTGTAAAATTTCATTTTTTAAAAATGCCAAATCCTGTTAGATTTGTTTACCGTGTTGATTTGAGAAGCCCTGAAGAAATTTTTGAACATGGCTTTTCAACTTTAGGTGATGTGAGAAATTTCTTTGAACACATTCTCTCCACTAATTTTGGTAGAAGCTATTTTATTTCCACTTCAGAAACACCCACAGCAGCTATTCGCTTCTTTGGTAGCTGGTTACGGGAATATGTACCAGAGCACCCCAGAAGGGCTTACTTATATGAAATTCGTGCCGACCAACACTTTTACAATGCCCGCGCCACTGGGGAGAACTTGTTAGATTTAATGCGTCAAAGACAAGTAGTATTTGACTCTGGTGATCGAGAAATGGCACAAATGGGAATTAGAGCTTTACGCACTTCCTTTGCGTATCAACGTGAATGGTTTACCGATGGTCCAATTGCAGCAGCTAATGTCCGTAGTGCTTGACTAGTAGATGCTGTTCCCGTTGAACCTGGTCATGCTCACCACCCGGCTGGTCGTGTTGTAGAGACTACTAGAATTAATGAACCGGAAATGCACAACCCTCATTATCAAGAGCTGCAAACCCAAGCCAATGATCAACCATGATTGCCAACACCAGGAATAGCTACTCCTGTACATTTATCAATTCCCCAAGCAGCTTCCGTTGCTGATGTTTCGGAAGGTACTTCCGCTTCGCTATCGTTTGCGTGCCCTGATTGAAGTCCACCTTCTAGTAATGGTGAAAATCCGCTAGACAAATGCATTGCGGAAAAGATTGATAACTATAACCTACAATCCTTACCACAGTACGCTAGCAGTGTAAAGGAACTGGAAGATACACCAGTATACCTAAGGGGAATTAAAACGCAAAAAACCTTTATGTTACAAGCAGATCCGCAAAATAACAATGTCTTTTTGGTCGAAGTAAACCCCAAACAAAAGTCCAGCTTTCCCCAAACCATCTTCTTTTGGGATGTTTATCAACGAATTTGTCTCAAGGATTTAACTGGTGCACAAATCAGTCTTTCGCTTACTGCCTTTACTACTCAGTATGCTGGTCAGCTCAAAGTGCACCTTAGTGTTAGCGCGGTTAATGCCGTGAACCAAAAGTGAAAAATGACACCGCAAGACAGTGCAATAACTCAGTTTCGGGTCTCCTCTGAACTGTTAGGTCAAACTGAAAATGGCTTGTTCTGAAATACCAAGAGTGGTGGTTCACAACACGATTTGTATGTATGTCCTTTGAAAAATCCACCTAGTGATTTGGAAGAATTACAAATAATTGTTGATGAATGTACTACCCATGCGCAGTTTGTTACTATGCGTGCAGCTAGCACCTTCTTTGTTGATGTTCAGCTAGGCTGGTATTGAAGGGGTTATTACTATACCCCACAATTAAGTGGTTGATCTTATCAGATGAAAACACCAGATGGACAGATATTCTATGATCTAAAAACTTCGAAAATCTTCTTTGTCCAGGACAACCAAAACGTGTTCTTTCTCCATAATAAACTCAACAAACAAACTGGTTACAGCTGGGATTGAGTAGAATGGCTAAAACATGACATGAATGAGGACAAAGACGAAAACTTTAAATGGTACTTTTCGCGTGATGACCTTACCATTCCTTCCGTTGAAGGGCTTAACTTCCGCCACATTCGCTGTTACGCTGACAACCAGCAGTTAAAGGTGATCATAAGCGGTTCACGTTGGGGCGGTTGGTACTCCACTTACGATAAAGTTGAAAGTAATGTCGAAGATAAGATTTTGGTCAAAGATGGTTTTGATCGCTTTTAGCGATTAAGCTTTAACGTCACTGTTTTGCTCTAATGTTAGAAGCAAAGATCTTGTCGACAAACTCGTTGCGCTTGCGGTAACGACGTACTACTAAATAAGTTACAGCACCAGCAGCTACTACACTAGCTATAGCAGCCACAGTTAAACCAGCTACCAACGGATCTACGTGGGTTAAGAAGTTGAAACTGCCACCATTTGCATTCAATTCAACGGTAAAGCCATCTAGTGATCTTTCACCTTGAACTAGTTGTTGGCTAGACAATGAGCGTGGTTTGAAGAGGTCAAATGAACCATTAGCACCAACTTGGTTTTCGTACTTAACTTGTAAGAACTCCTTCAATTGTCTTTGGAAGGTACTTTGAATTGGTGCATAAGCATCATTAATAAATGAGCTGTCGGTAAACAATGCCCCTACAATCATGTTGTACTCATACGGATTGAGTCCAGAGAAATCAAACACAAGTGTTTTCTGCTCCTTATTATTTCCCACACTTCTATTTTCTATTTTGTAAGGAATATCAACCGTAGTGCTTTGGTTTGGGTTGAATGCATTCTTAGTTAAGGCAATTCTTAGATTACCACCTTCAGCATTATTGTCAGTTTGGGCACCGCCATCACTTACCATAACTGTGTAAGATTGTGCTTCCTGTGCCAATTTGTTGACATCATTTGGGTCATTCAAACCTAAAGAGCCATTTTGTTCAAGCGAGAAGTCGGCTTTTAAGTCTAGCTTGATGCCATCCTTTTCAACATCAAACTTAATTTCGTCCTTTAAGAAAACCTTCTTGTTAGTTAGTCTCTCTACATAACCAACCACCGCACCACTAGCAGCTTGACTTAAAACACCACGTTTAGAACTCTCGTTGGACTTCAAGAAGTGCGCTACGATCTCTCTGTTTTTGGTTGAAAGAGAAGTGGTTGTTTGGAAGAAGTTTTGCGGTAATTTTCCCCTTCAATCACTAGTGTTGAAATACAGTTTAGATTTTTGTCTTGCTTGAATTACAGCGTCCCTTGAGGCATTATCGACGTATATACCGTTGTTGTCGAACCTAAGTGCATCGAGTGAGTAGAACTTATCCTTTGTTTCAAAACGGATAATGTAAGTACTTTGCGCGTTCTTGTCTAAACCTTCTAGGTAATAGAAATAGTTTCTAGGGTTAGCAATACGACGTTGGTTACCAAGGTTGTTAGCGTTAGTTACATCTACTACCACATAGAAGTGACCATTGCTTTCATTTCCGTTCAAGTTAATATTGTTCTTAGTTTGAAAGTTTTGCAGTGCAGTTTTGAGTTGAGTATCTTTAGTATCAGTGCTGCCTAAAACATCTTGAACTTTGCGTACGTTATCAAAGTCGAGGTTAGTGCTAATACCGTTGCTGGTAAGGTAATCGTTAATGCTCTTGTTGAAACTCTTGTCATCAATTTCACGTAACTGATCATTTTGTGCTTTTCAAACAATACTTCTTGTCTGTAAGTTTGCCCGTTCTCAACTAGCACTTTGGAAATCTCTTCTTCAGTTAATATCTGAAGACTAGCTTGTTGTCTTCCTTCTTAAGTAGTGTTTTAGCTACAGATACAACCTTATCTTCTTTCACTTCCAAGCGATCAAGGAAGTCATTATCACGTCAGCCACCATCTTGAAAACCAATTGCATTAACAATAATTTCAGCTTTGTTTTCGTTTGCTAAAGCATTGAGCTTGGTCTTCATTGTTTTCAATTGCTAAGAACTGGAAGTTTTGTCCATCCCACACTTTAACTTCCTTAGTTACTTTTTGTCCTAGGGTAACGTTAGCGTAAATGGCCATGGAAAAGTAAGAAGCTACTAACTTTAAGAATTGATCTCAGTCCTTCGGTTCTTGGTTTAAATTAAGACCTAATTCCTTATTTGCTTCTTGATACCACTTAACTCAACCGGTTGAGTTAATTGGAGTACTTACTTTATTGGTATCTTTATCTAGGCTACTAATGTATTCTTTTACCTTTAGGTAATCAACTTGCTTGTTTTTAATTTGCGTTAAAACGCTGTTAATGCCAGCTTTTTCACTTTCGTTACTTTTGCTCTCTAAACGTTGTTCTAAATCCTTTACTTGTTCAGAAAGGTTGTAAACGTTAGTGATTGCATTAACAAAAACTGGATTTAAAACAAAATCTAAGGTAGGTGCTTGAGTTAAAACACCGTTTCTAGTTGAATTTAGATTAGCGTTTGTTTTAGGGATCAACAGCTTATCTAAGTTAAAGTTTTTGTAACGGACAAAACTAAAGCTTTGGTTTTGTAGTTTGTAATTTCTGTTTTCAAACTTAAAGTTAGTTCCAGTGGTGTTAGTGTCAATCACCTCTTTCACTGCACTACCTAACCCAACATTTAGAGTTTCATCCTTGCGTACGTATGCTTTAAGTTGACTTTCAGCTGCACTTTGATAGTTGGCCTGTGTACTCTTAGCGTTAGCAATAAAGTCAGCTGTTTTTATCGATTGCTGCTGACCATTGCTGTCGGTGTAAAAAAGCTTGGTTCAAGGATCTAACTTAACAAAGAAAGTAATGCCGTGATTAGTAAAGCTCTTTAAAGGACGGTCGGAATAATGAATTGTTGCAGGGTAATCTAATGGATCACTGTTAGCACCTAAGAAAGTGGTTTCAAAGTTTACGTTAGGTTTTTCAATGAAGTTACGATCTTTGTTTGCAAGTCACTAGTTTCTAGTAGTTGTAAAACCAGATTGACGGAACCATAAGAATACTCCTTTCTCTGAGTGGAGGTTGTTGACGTTTTGTAACAGCTTTTCTTTAATGCTACCCCAAACGGTTTTGGAAGTTTCGTTTGTTGCGGGGTCAGATTTTTTTAAATCTGCTTGACGGTGAAGTTCACCCCTTTCAATGAGCACTATCTTTTGGTGGGCAAAGTCAAGGTCAAACCAAGCAGTTCATCTCCATCGATCATCATTGGTTCCAGCTTTTTTTGCACCCCGGTAGTGGGTTTTGTAATACTTGAACTTATCATACCCTGCTCGGTATTTTGTTTCATGTCGAAACGGCGATTTCCAACCGTTATTATTAACAGTGCCCACTAAACCAAAACTCATATCTGACTTAGTTTAGATGTTTACTTCTTTTATTCCTGGAAACTTGTTACTTAATTTAGAAAAGGAAGATCCACCAACAAGCCAACCAAAACCAGCGAGTCCAGCACACAACAGCAACGAATAATTTCGTAAACGCTTTATCCTCATACGTGTAAATTTATAACAAAAAAACGCCCCTTGGGGAACGTTTTTAAACAAGTTTAATTATTAGTCATTTTTATTGAATATTAAATCTTCATTTATTTAATGCTTAAAGATTAATTAACTTTTGCACTTGCTTAAGAAGTCCATTTTGACAAAGAAAAATCCCAAAAGGATGGCAATAAAGCTCACGCTTTTGGGATCTTCCTTGTGGTTTAAACCATTTTTTTAGCTAAGTCTACTTAACTTTTCTTGCTTTGAGCATTTGTTCTCAGAAGTGCTTACGACTCTTACGTCAGCGGTGTAAGACAACACCACTAGCTATTATTGTTGAAAGTAATGCAGCACTACCAGTGAAAGTAATTGCTATTAATGGTCAGTTAATCTTATTGATGTCTTCTTTATTGAAAATGTTTTTGTGTAGATCAAAGGTGAAGTTATCAATTTGTTTTTGGTTATTTGCCAATTGTACCTTGGTCAATAAAGGTAGTTGACCAGTCTTAATTGGTGCTTGGTATTTGTGTTCTAAAACGGTTTTGAGCTGCTTTCTAAATTCTGTTTGCAATGGAGCGTAGGCATTCTTAATAAAGGAGCTGTCGGTAAACAACAGACTAACAACTGTGTTGTATTCGAAGTTGTTTAGGTTAGAGAAGTCAAACACCAAGTTTTTCTGCTCTCTAACCGTACCATCCTTGTTAGTTTTGGATTTCTTGGTAACAATCCAGTAAGGCAGTTTAATAACGGTACTTTGTTCAGGGTTGGTGGTTAAAGTAATCTTGAAGATGTTTTTGTCTGATTCTGGATCATCACCTTTTTCTTCTGAAACCAAGACATTGTAACCCTTAGCTTCTTCTACAATTTGGTTAATTACACTTGGGTCTTTAACCTCTAAATTACCCTTCTTGTCTAAGGTAAAGTTCGATGTTAAACGTAGCTTTAAATTATTTTTGTCTTCCTTATTAAAGCTCACCCTTTCCTTTAAAAAGAGCTTTTTACCAGTCATTCGCTCAACATAGCCTAAGATCGAACCACTGACATTTTCACTCAAAAAACCCTCATCATTCTCAGCGTTAGGTGTAGATAGTTTAGCTGAATTGTCAGCACTTGCTGTAGGCAAAGTAAGTTCAGCGTTGGTGAGGTTAGCCTTCAAAGCAGCGTTCCAGTTATGAGTATCGAGATAAAGGTTTTGGTTTTGCTTGGCTTGGATAATTGCATCCTTGGACACATTTTTCACATAAAGGCCACGACTATCAACCGCTAGTGATTCGAGTGAATACAGCTTTTGTTTGTTTTCAAAGCGCACTAAGTACGAACTTTGTGCTCCTTTGTCCAACCCTTCAATATAGTAAAAGTAACTCTTAGCGTTATTTGCCCGGCGTTGGTTACCCAAGTCATTGGCATTAGTTACATCCACTACTACATAAAACTGACCAGTGTATGGGTTAGATCGTAGGTTTAAACCATACTTGTTTGCCGTACTTTGAACGCTAGCGAGCTTCGGATCATCACTTTTGCGGGCGTTTAACATCGCATCAACTTTATGAACAGCATTTACTTTAATTTCAGTTTCAGAGATGCCTTGTACCTTTAAATAGTTGTTAATGTCCTTTTGGAAGCGTTCGCGCTCATCGTTCAAATCAGTGCTTTGTACTACAATACTCTTGGTTTGTGAGTTTTGTTTGTCCCAGCTAGCTTGTTTAAAGTTTTGTCGGTAATGGTACTTATAAGAAATTTCGTTGGCTGCTTTTTCTATTTTGCTAGCATCACCGTTAGCAATTTTAGTTTTACTACTCTTAGGGGTTAGAGCTACCTTATTGATAAAACTAGCATCACGTAAACCGCCTTCATCTTGAAAACCAATGGCGCTTACAGCAATCTCAGCGCGGTTTTCATTTGCTAGTTGTTCTGATTGTTTTTCAGTGTTTTCAATCGGGATGAACTCAAACTTAGCACCATCCCAAACCTTCATCCGTCTTTCCTTTGCGCCGTTTAATTTGACGTTAGCAAAAATGTCCTTAGAGAAGTAAGAAGCAATTAAGCTGGAAAGTTGTGTTCAGGTATTAACATCATCGTTCAAGTTTAAACCAAACTCTTTTTTAGCATCAGCATACCATTGTGCAGTATTTTCACCCTTGGATTCAATACTACCTGGATTTTGGCTTCAATTTTTCAGCTTGCCAATAAAATCAGCTACTTGAATGTAGTCAACTTCCTTATTTTTGATTTGTTCTATAACCTTATTGATTGCTGCTTTGTCACTTTCATTGTAAGGTTGATCAGACTTTAAATGCTCCTTTAAATCAAGTGTTTGCGCAAACAGATTGTGAATGTTGACAATGGCGTTTGTCAAAACGGGGTTAAAGTCAAAGGACAGTGTTGGTTGTTTGACAAAAACACCATTCTTAATTGCCGTAGCATCCTCGTTGGTTTTTGGCACTAGTAACTTATCGAGCTTAAAGTTATTGTAAGTGTCAATGCTAAAGGTCGAATCCTTAAGTTTGTACTTTAGCTGTTGGTTGGAGGCTATATCACTCTTAGTGGTGTAAGTTTGACGTACAGCACTACCTAAACCTAAGTCAATACTGCGAGTTTTTCTAACAAGCGTTTTAATCTGGTTTTTATTAACCCATTGGTAATTAGTCTTGGTACTCTTTGCTTTTCTAATGTAATCACTTACAGTAATTAATCTTTCCTGACCGTTTTCCTTGTAAAACAGTTTGCTGTAAGGTTCTAGCTTTAAAAAGAAGCGCGTGTCAGTGGTATGACTACCTCAATCAGGACTGTGCCAATTGGAGTTACCACCTTCAGAAGTGATGTTGGTGGTAAAGGCATTGGGGTTATTGTTGGTATCCCAAAAATCGGTAAAAAAGCCACTGTTAGCCCAGCCCTTAGTACCCTTGGTGTTAAAACCAGTTTGGTTGAACCAGATGAAGATTCCCTTATCTGGATCTAACCGCTCAGCACTGTAGAGAATCTTCGAGCGAATATCACCCCATAAAGGTTTGTTTTTATCCCATTTTCTGGGAGTTGGTTCTGGAACACGCTTGTCAATATGATATTCATCATTTTCCACCAAACCTATGCGTTGGTTGGCATAACCCAAATCAAACCAAGCCACCCACTTTCAGTAATAAGGGTTACTTGCTGTCCTTTCGCGGTTAGAGTACTTGTAATATGTTTGGTTCCAGCTAAAGTGAACGTTTTTGTCGTATTTGTTCTCGTGCCGGAACGCTCAAGTGCGACCGTTATCAGTGTGTCAACCCACTAAACCAAAGCTAGCGCCAGAGTTGGTATACACCTTTACATCTGGTAGTTCACCGTTCTGAATGGAAAGCTGAGCAGTACGCTCAATTTGCTTAAGTGCTTCTTGAGCGTAAAGGCTTTCACTAATGTAGTTATTAACGTTGTTTAAGTAGACCTCTGTCTTTTTACCGTTATTTCACTTTAAATCAAAGCGCTGGGTAATAGCTGGACTTTTACTCTTTAAGATTGAAAGCACATCGCCTTTACCCTTAAGGTAGCGCATGTGAACGCCACCATCGCTTGATAGTGTTTCATTGTTACTTTTAACAACGCTAGTGAATGCTGAACTTGCAACGAATCAACCAAAACTTCCTAATGCAAAACCAAAAAGTAAGAATCGGTACTGTAAACGTTTCATGCTTTATTCTAGCACTTTAAAAATATTTTTGACAATCCTTCTGCGCGTGTATTAAAGCAAATGCTGAACGAAATTGACAAAAGACGCACGGTTTGAACTCAAAAAGCTTTTTATGGCGTGGTATTTCACCAAGCATTTCATTCATTTTACCCTCTAAATGAAGAGTTACTTTGCTCTGCTTATCTCAATATAGTTAAAGTAATGTGTATAAGCGTAGCTAAAATAAAAAAACCAACTGAGAGCAGTTGGTTGGAATCAATAATTTGGCGCGCCCAATAGGACTTGAACCCATAACCTTCTGGTCCGAAGCCAGACACTCTATCCAATTGAGCTATGGGCGCTTCTTATAGTTAATTCTATTAAATCTCTGAGAACTGCGCAAATCTTTATTAAAATTAGCTGACTTCAACACAGTCGCTCTAATACATGTCAAAGGATAAAAAAAATAAGGTTGAACAGCTTGAACCAGTAGACCTTTTTGAGCGGACTAAGCTAGAGGACACCCAAGTTTTAAATGATGTTGAACTAGATGACATCAAGAAACTAGAAGAGTTGAAAAAAGAATTGGAAAACACCTTTGATCCACGTACCAGAATTGAAATTAAACGTGAAATCAAAGAGTTGGAACGTAAACTAAGAAGGAACCGTTAGTGAATGTTTGTTAACCTCCACACTAACTCTTACTATAACTTCCTCAACTCCACACTTTCACCGCAAAAGCTAGTTGACCTAGCTGTTCAAGACCAACAGGTAGCTGTTTGTTTAACCGATCCCAACTTATTTGGTGCTACCGAGTTTTTTTTGGCCTGTCAAAAAGCGCACATTAAACCCTTAATTGGGTTGAGTGTTACTGTTCGACATTACGAACAAAACGTTAATTTACTAGTAATTGCCCAAACCAACCGGGGTTATCAGAACCTAATGTGCTTAGCGTTAGTTAAAGACCAACCAGATTTGCAATTAGAACCCTTTTTGGATGGTAATGTGGTAATTATTTGCACACAAACTGAACTGCGCCTCAACACCGCAAACCCCGTTTACTTAGCACATGGTTTGAGTGGTCGTTATCCTAAAATTGCAGTAACACAAAAACCAGTTAAGTGTCAAAACACCAATAAGGACTTAACACTGCTCTTAACACTTAAGCAAATTAGTCAAATTAACAGCGAACACTTCCAGCCACTGGAATGAAAATTAAGCCGTAGTTTAAACGAAATCCAATTGGATCCACCATTGTTGCAACAACTCCGGCACCAACCTTTTTTAAGCCAAAAGGCAGCACAGCAAATTTTTAGCGAAGAGGAATTGGGAAATCTGCAAAAGCTAGTGGAGCAAAGTCAATGAGATTTAACCAAACTCAAAGCGTCATCGTTACAAGTGTCACACAACGATGCACAAATGTTAAGCGAGCAGTGTCAACTAGCCTTAACTGAATTTTTAAAGCTCAATCCGCAATTAAACAAACAGCTCTATGAAGAGCGGTTAGCCAAAGAATTGGAGATAATTAACTCACTCCACTTTGCTAGTTACTTTTTAGTGGTGAGCGACTTAGTTCAGTTTGCCCATAATAACGATATCCTCATCGGTCCTGGTCGAGGATCTGCAGTGGGTTCTTTAGTGGCCTTTCTGCTCAAAATTACCCAAATTGATCCAGTAGCCAACAATCTCATTTTTGAGCGCTTTATTTCCCGTCACCGCCAGGGCTTACCTGACATTGACATTGACATTATGGAAACTAAACGTGATTTAGTAATTGACTATGTCATGCAAAAATATGGCCGGGAACAGTGTGCACAAATAGTTACTTTCCAAAAATTTAAGACCCGCTCTGCGCTGCGTGATGTCGGTAAGGTATTTAAACACATTGAGGGTGCTGAAGATTTACTGGGTAAACTACCAAAAGACAAAAGCTTATTGGAGCTTGATGTAAACGGTGTTACTGATCCGGTTTTACAACTTAGTTTAAAGAGCTTTCGCTTGTTGTGGGAGGTGGCGCGTGAAATTATTAACTTTCCGCGCCAACCAAGCATTCACGCTTCTGGTGTTGTGATTGTCACTGAACCTTTAATTACCACGATCCCTTTAATGGTGGGTAATAACAACAACTACGTCACCCAAGTGTCGATGGACTGGTTAGAATGGTACAACCTCAATAAGTTTGACTTATTGGGTTTAATTAACCTCACCATGATCCATGAGGTTGTGCAAGCGGTAAAACCCAAGGAAGTTAGCGTACAGCAATTCCTCCAACAAATACCGCTAGATGATGAAGCTACTTTTACTAACCTCACTAACCAAGCCACTTTAGGTGTTTTTCAACTAGAGTCGTTTGGGATGAAAAAGGTGCTCAAACAAATTAAACCGCACAACCTCCATGATTTAGCAATTGTTTTAGCACTTTACCGCCCCGGTCCACAGGACAACATTAATACCTTTATTGCTAACCGTAACTTAGGGTTTGACACATCAGACATCGATCCGCGAATTTTGCCAATCCTCAAAGAAACTTACGGAGTGTTAATCTTTCAAGAACAGGTTATTAACATCGCCAAAACTGTTGCTAACTATTCATTGGAAACCGCTGATAGCTTTCGCCGTGCCATTTCCAAAAAAAACTTGCAAGTAATTCAGGACAACATGCGCAGCTTTTATGAAGGCGCGCTAGCCAATAACTTTAGCTTAAAAGCAGCGACTACTATCTTTAATTACATCCAGCGCTTTGCTGGATATGGTTTTAACTTGTCCCACGCCTTGGGCTATGCTTTATTGAGTTACTGAACAGCATGATTGAAGACACATTACTTTGAGCAATTTAACCTGTGGTGATTAAACCACGAACAGGGCAAAAAGGAAAAGCAAAAACAGCTCTTAAACGAGTTTATTAGCAGTGGTTATGAAATTTGCCCACCACTAATCAATAAGGCTAAAAGTGATTTTAGTGTTCAAGATAAAAAGCTGTATTTAGGCTTTAAGTTAATCAATGGTATTGGTGACAAGCAAGCCCATGCCTTGGAACATGTTCAGGAAGTTTTAAAGCAAAACCCCAATTTATCCTTAATAGCTACCGTTAATTTGTGTTTGAGCAAAACGGTAGGGGGTTTAGAACTTAAAGACATTACATTGCTCCAACAAGCAGGTTGTTTTAATTGCTTTAATTACACGGTTGACTTTAATTTGGCCAAAAGCTTTTGGGTGCAAAGTAACCATGAATTATTTCCAAAAATACCACTAGATCAACCACCCGTAATTAACTGAAAGAGCTTTGGTTTTTAATTTATGAAAAATGCAATTTTAATTGATGGTAACTCACTAGCCTACCGTGCTTACTTCGCTACTTGACAACAGGTGGAGTTTGCCAAACTACACAACTTACCCTTTAACAATGCGATTCGCACAATGTTAATGATGTGCTGAAACTTACTGCAGTCAAAACAGTATGACTATGGTGTTATTAGCTTTGATACTAAAGCGCCCACTTTTCGTGATCAACTTTACAGTGAATACAAAAGTAAGCGCAGTAAAACACCGAGTGAACTGTTAGTCCAAATTCCTGTTGTTAAAGAATCACTGCGTCATTTAGGTTTTTTAGTGTGTGAACAAGACGGGTTTGAGGCAGATGATCTAATTGGTAGTTATGCGAGGTTAATGACACAAAACAATGTGGCAGTAGACATCTACAGCTCTGACCGTGATTTACTACAGTTAGTCGATAGTATGACTAGTGTTTGGCTTTGTGTAAAGGGCACAAAAGAGATGAAGGAATATAACACTGACAACTTTGCTGAGCAGTTTTTTGGCTTAACACCAAAACAAGTGATTGAATATAAAGGTTTGGTAGGGGATAACAGTGATAACCTCACTGGCATTAAGGGAATTGGTCCGAAAAAAGGGATTGATCTACTCAAACAATACGGTACCATTGACAATATTTTTGCTAACTTTGACAAGCTATCCAAAGCATTGCAAACGATTCTCCAAGGCCAAATTGACACTGCTAAGAAATTCAGCTTTTTAGCGAGCATTAAAACAGATATTAAACTTAATGACGATATTGTCCACGCTGCTTTAAAACCAATCGATAAACAAGCTTTATTAGAGCTTTTAGATAAATATGGCATTAAAGCACTAGCGCAAAAATTTAGTCAACTGTAATGCCTGAACTACCTGAAGTTGCCACTGTAATTACCGAACTTAAGAGCTGTGTTTTAAACAAACCTGTCAAGCAAGTTAAGGTGCACTTAGACAAGGTGTTGAAAAATACTAATGTCAAGCAGCTTAACGATGCGTTAGTCAACCACAGCTTTGTGGACATTAAGCGCCGCGGCAAGTACATTATTTTTTGTTTAAGTAACGGTTTGTTTTTAGTGTCACACTTACGCATGGAAGGCAAATATTTCTTTGAAGCTAAAGGCAGTCAGTTTGACTTAAACCATGTTTTAGTCGAGTTTTTATTCCAAGATGGTGATCAACTTAACTATCATGATACGCGTCAATTTGGTACCTTTCACTTGTTCAATAGATATCAGTTTGAAAACGCACGTGAATTGAACAAATTAGCACTCGATCCCTTAGATCAGGAATTTAACCATCAAGCGATCTTTAACAAAGGCCATAAGTCCAACAAGAAGATTAAAACCTTTATTTTAGATCAGACCAACATTAGCGGTATTGGCAATATTTACGCTGATGAAATTTTGTTTGCAAGTAAGATTCACCCTGAAACTTTAGCTAAAAATTTGAACTTATCGCAGTATCAGCTAATTTGTCAAAATGCAACTGATATTTTAAAGAAAGCAGTTGAAATGAAGGGTACTACCATTGGCACTTTTACCTTTAAAAAAGATCATACCGGTGGTTACCAACACTTCTTGAAAATTCATGGCAAAAAGGGGAAGCAATGTCAAAGCTGTAACACCACTATTATTAAGAAAAAGATTAATGGTCGGGGCAGCTATATTTGTGAAAAGTGTCAAATACAGCGTTAATGGTTAACTGTTCCAGTTAATGCCAGAATTATGGCTTTATAAAAAAGAGCTTAAATATCTTAAACTTAAGCTCTTTTTGATCACTTAAATTAACCATAAAGATTGTTTTTAATCCTTAGATTAATCCACGCTTGTTAGAAAAAATATGAAAAACAAAATTAAATACGTTTACTCCGACCTGGATGGCACGATTGTGAGCTGAAATCCTAAAAACCAGTTTACCCATCAGGGCAAAACTTACAAAAATCTCCATGAAGTTAGTCACGCTACCGTAACTGCTTTTAAACAATTACAAGCTCAAGGTATCAAAATTGGAATTGTGACTGGCCGTGATTACTGTCGCGTTCGTTGGTTAGAAAAGTACCTAAACACTGATCTACCAACAATTACCCTCGATGGCGCCATTATTTACTTCCGCGATGAAATTATTCGGCAAGAAGTGCTTGATAAGGAATTTATCCATGGCATTAACCAGATTGTCAAACGTTACCCTACAGCAGCCTTTAAGCTCAACATGGGTTGAGGTAACTATTACACCTGCAATCCTTCTTTAATCTTTGAAGGTGACCATGCTTACCGTGAGCACTTTAATGCTGACAGTAAGTTTTATCGCAAAGAGATCGACAACACGGTTGATTGAGATATAAATAACATGAAAGTAAATCAGGTTTACTTTGACACATTTACTTGCCCTGAACCAATGATCCAAGAGCTCGATAACTTAGTTGAAAAAAGTGATGTAACCGCCAAAAGCTACCGCCATTCACTTTACATTATTAAAAAAGGGGTATCGAAGGCTAGTGCACTACAAAACCTCCAACGAGACTTTTTAGTGGAAATGAAGCCTGCTAACACGATTGTCTTTGGTGATGGGGATAATGACATTGAAATGATGCAGTGAGCTGATCACAGTGTGTCGTTAACTGGTAGTGATCCCGAGTGTTATAAGTTAGCTAAGTACCACACCGACAGCGTTGACGATGATGGGATTGCCAAATGGATTAATAAAAATCTGTTATGTTAATCGCTGTTGTAGGCAAAGCTGGAGTTGGTAAAACCACCGTTTTGCAATACATCGCTGACTATTTTCATTTTCCCGTTTTTTTTGCTGATCGGTTTATCCATCAACAATACGCAAATGGTCAAGCTGGTTATGCAATTGTAAAACAGCAATTTGGTGCTCAGTTTGTCAATCATGAAGCAGTTGATCGCAAACAGTTAGCACAATACGTTTTTAATCAACCTGATGAGTTAAAGCGTTTGTCCAACTTAACCAAACCCCTAGTACAAGAGTGACTCAATCAATTAAAAGCGCAATTCCAAGACAAAATTGCACTTGTGGAAATTGCGGTGATGCTCAATTACTGAAACGATTACCGCCCTTTCTTTGATGAAGTAATCCAAATTGAACGTGATGCTAAGATAGTAAAACAAGCACTAAAAGCACGTGGCGTTGATGTAGAGCAGGTGCAAAAGTTAATTGCTGATCCGACTTACCCAATTCTTACAGTAATCAATAACAGCACTGTAGCTGAGTGTGCTTTACACGTTACTCAATTTTTAGAAAGCATTGCAAAAAGTGATAAATGCCATCATGGTCATTATCAAACTCCGAAATAAAGGTCGCGTTTTGTTTTGCTAAATCACTACCGTTAGCCATTGCGACAGAGTACTTGACAGCATTAAAAATTTCCACATCATTGTCCCCATCGCCAAAGACCATGGTCATTTGGGGATCTAAACCGGTTTGCTGTTCTAATGCTTTGAGACCGTATGCCTTATTGACATCTTTGTGCATAATGTCAAGCGCAAAGGTCATAGAACTGACATAGCTAATGTTTTCAATCGCATCGAGATGTTTGGTTATTTTACTAATGCGACCCCTGTCTTCAGTGATTAAAAGAATCTTGGTAATGTTTTGTGTGGCAAAATCAAACTTGCTAGCATCAGTTAACGGATTAATGGTAAAGTTATTTTGTGTAATGTCAGGTTCTACAATTTTGCTTTGAGTAGTGCGTAACCGTTTGCAAGGTATACCGGGTTTTTCAAAACCAAACACCTTTTTATCAGTGTAAATGCAAAAGCTTTCTTCCAATTGCAACAGTAAACGCATAATGGGTACCACACTTTGGGGTGCTAACTGGGTCATATGCAGCGCTTTTTCCCGAGCAAAGTCATAAATTAAAGCACCGTTAGAGGAAATAACCGGTAGATCAGGTTGAATAAACTGAGTGGTATTGCGGATTAAGATATGACTGCGCCCAGTCGCAAAGGTAATTTTAAACCCCTGCTTTTGCAACTCTTTGAGAAAAGTAACAGTTTGTGGTAGTGGTTCATGACCCCAAGACAATAAAGTACCATCGAGGTCAAAAATTAAGTTTTGTACTTTTGGTTTCATAGCTATTCGGATTTTAAAAGAACATTAATAATACGATTAGGGACAAAAACAACACGCGCTAAGGGTTGGTCGAACAACGCTTGGAATTCCGGATCTGTCTTAAAGGCTTTTAAGACAGTCTGTTCATCAACCCCTTTAGCAAATTCCTTGGTACCTTTGAAGCGACCGTTGACCGAAAGAATAATTTTAACCTTATCGGTTTCAAGTACGCTGTTATCAACATTTGGTAGTTGAGCATGAACAATAAACGGCTCCATCCCTAGTTTAGCGTTTAATTCTTCCGCTAAATAAGGTGCAAAAAACGATAACACCGTCAAAAAGCCCTTTGCATAAGCCAGGGTTAATTGTTTTGTTTTGTAAAGCAGGTTTAAAAAGATCATCATTTGGCTAATTACCAAATTGAGTTCTTGTTTCTCTAAGTGCTCGTAACTACGCTTTAAAAAGAGGTTATAACCATAAACAGTTTCTTGATCTACCGTTTCTTTAATAATGTTTTCCTTGTGAAAGAAATTGTGCACCCGATCAAGTCAACGCCGCATTCCGTTTAACCCATCATCCAATCAGGTCAACGCCGCGCTAATCGGCCCCATAAACATCAAGTAAAGTCGCAAGGCATCAGCACCATGAGAATCAATTAGTGGCGTAGGGTTGATTGTGTTGCCCTTGGATTTGGACATCTTTTTGCCATCTGGTCCCAATACCATGCCCTGGTTAATTAACTTTTTAAAGGGCTCTTTAGTGGTGACTATTCCCTTTTGGTACAAGAATTGGTGCCAAAAGCGCGCGTACAAGAGGTGCAACACGGCATGTTCGGCACCACCCACATACAGATCCACTGGTAGGTAACGCTCAAAAAGATCTTTCGCTTCCTTGGAGTCAATTGGCCAAAAGTTTTCGTTATTAATTAACATCAAATAACCCAAGTAATACCAACAAGAACCAGCCCACTGGGGCATAGTGCTCGTTTCCCGAGTAAAAGTTTGATTACCTTGTTTCACCTTAGCCCACTCCTGGTTTCGTCACAATGGTGGGTTAGTCGAACCATCAGGTTGATAGTTAGCCAAAGCTGGTAGGGTAACGGGGAGCTCTTTAACTAAATGGGGTTGGTTGTTTTCGTCAAACAAAATCGGAAAAGGTTCACCCCAGTAACGTTGACGGCTAAAAATCCAGTCACGGAGTTTGTACACCGTTGTTTTTTTAGCTTTTTGACGCTCGACTAACATTTGCGTAATTTGGGTGTTAGCAGTTTGGGAGTTGAGCCCGCTAAACTGCCCGGAGTGTTGCAAGCGTTCCTGTTTATCAATGACGGTAATAATCGGTAGCTTTTGCTTCCGCGCGAAGAAGTTATCGTTTTCGTTATGCGCTGGCACACCCATCACAGCATCCGTACCATAACCTTCCACTACATAGTTAGCTACATACAACGGAATGGCAGTATTAGTTGCTGGATTAATTGCTTTAAGTTTTAAATCAACCCCGTCATGGAGCGTCTCAGGTTGCTTTACAGTGGTGGTTTTCTGTGCTTGTTTTTGGAGAAACTGTGCTATCTTGGGGTTGGTTTTAGCTACCTCTTTTACTAAGGGATGGCTAGTGGAAAGCGCTAAAAAGGTTACACCAAAAATAGTTTGCACCTTAGTGGTAAAAATGGTAATCGTTTCCTTGGAGTTTTCTATGTCAAACGTTACCTCTACCCCTTCACTTTTACCAATCCAGTTGGTTTGCATTTCCTTAATAGATTGATGTCAATCCAGCTCAGCCAAACCATTGAGTAAACTTTCAGCAAAAGCAGTTATTTTCAACACCCATTGCTTCATTTTTCGCTTGGTTACCGGGAACTCGCCACGCTCACTCACCGCATTACCATTACTGTCAATCAACACCTCTTCGTTGGCTAAAACGGTGCCCAATTCAGCACACCAGTTGACATCAATCTCCTTCACTTCAGCCAAACCGGCTTCAAATAACTGACCAAAGATCCACTGTGTGTACTGGTAATAGCGTGGATCAGTTGTCTTTAAAGACAGCTGGTAGTCATAATCAAAGCCAAAGGCCGTTAACTGCTCAATGAAATTAGTAATGTTTTTGTCAGTTCAGTCACCAGGATGCTTATTGGAGGCAATCGCGTATTGTTCTGCTGGTAACCCAAAAGCATCAAAGCCAATCGGGTGAATCACATTAAAACCCTTCGCTTTATGATAACGACTCAAAACATCGGTAATGGTGTAAGCGCGCACATGTCCCAGGTGTAAACCAGCCGCTGAAGGATAAGGGAACATGTCCAAGATATAGTATTTGGGCTTAGTTTTATCATCAAAAAAACGGTTAACTTGCTTCGTTTTTCACAATTGCAACCATTTCTCTTCCAGTAAGTTGTGGTTATACATCTAGTTTTTTTTGAACGAGCGCTTAATTAAATACTTAATTAAATGGTAGAACAGGTCAGCTATAATGGCTACAGCAATCCCAAAAAGTAAACCAAAACCAACTGGAATAAAGAAGTGCACCAGGGTTTGGAACAGTTTTTGCTGTTGCTCTACAGTGCCTTTAAATCATTGATTAATATTGGGAATAATTTGCACAATTAGCCCACTAGCATCCTTTTCAGCTAGTAAATTAGCCCACTCCTTAGTATTGTAGGTATTGAGCCAAAAAGAGCCAATAAAAATAATAAAACCAACCGCGACAAAGAAGAGGAAAATCGCTAGTTTAATGAGCCGTTTAAGCAGCATGGTAAGTGGAAATTAAGCTAATTAAATTATAAAGCTAGATAATTTCCCAGTCTAAGTTTTTTGACTTTAATACCTCTAGTACTTGTTTGGTGATGGATTCTACTGATTGGTTAGCGTTTACTATGCAATGGCTAATACCAAAGTCATAACAAACGTTTTTAATAAATTCACCATAAATCTTGTACAGGTTCTTAAAGTAGTCCTTATTTTTGGCAAAGTTATCAATTTCTACTTTACGGTTACGTTGAAACAATCTTTGTTCAAACAACTCCCAATCACCTTCTAAGATAACGTACAAAGCGGGAATACCATGTTTAAAGATTAGTTCTTTAACAATCCCATTCCACAAGTGGTTATAGTAAGTAAAGATGGAAGGACGGTGGATGTTTTGCTTGGCGAACAATCAATCTTCAAAGATCGAGCGGTCAAAGATAGTTGGTGTTTTATTGTTACATTCCTTGCGGTATTTATTAAAGCGGTTTAAGGTAAAGTACAGTTGAAATAAAGGGGCGTAGAGCAACTCGTCGTTACGCTCATACATTTTGGCTAACAATAAATCAGCTAGCTGGTCTTGTTCTTCCAGTTCGTAAATCACCTTAGAACCCTTTAAGTGTTTAGCTAAACTTTCAGCTATCGTCGTTTTACCAAAAGCAATCATGCCACCAACCACAACGGCGTTAGCAATCTTAGCTGGTTGAAATTTGGGTTTTAAGGGAGTCTTCATAATACTTAGAACGTGGTTACAGTGCAAAGTATTATGCAATAAAAAACCATCACACTCATTAAATAGTCTTAATGCTGCTCCATTTCTGGCCTGACATGATTCGACCGTAATGATGTGACGGCTCTAAATTCTAACACTTAACTGCTTTTAATCAATTAATTTGCTTACGCGCTTAATCGAATTACGCTTCGGCTGGGTCGCGCCGCGAGGTAGATCTTTATTGGTTGTTGGTGGAGTTTCTTCCACTTCCATGTCATCATCCATATCATCGAGTGTATAGCTAATAACTTGGGTTTGCGGGATAACAGCCTCTTGAGGACGGTGGAATTGCTGTGAAGTGTCAGCTAGGCTAACGGTATGGGTCTGACCATTAACTTGCGCGTTAGTAATTTCACCTAACTTTTGTTCCAAGCGGTTTTTGAGTTTGTTGTACTGCTTGACCAAATCGTTGTTAGCGCGCTGAAAGTCACGCTTTTCACTGGTAAGTGAATTGTTAATTTCCTGAATCCGGGCTAAATCGACTTGCAAGTCACTCACCATCTTTTCGTACTTTTGGTTTTTAAACAACACTAGTTTGTGCTGCTTTTGCAACGCCTCATTTTCATCAACCGCACTTTGCAAGTAAGTTTGCAGTTGGGTAATCTCACTGTTCTTTTCGTTTAACTTACTTGTAAAGATGGCCTGGATTTCCTCATCACGCCTGTGAATGGCATCAGTACACTGCTGCTCGTACTCCTTCAACGCTTCTTCCAGGGTTTTGTTGTAGTGTTCCTCAAGCTTGTGGTTGTACTCCTTGGCTTCATTGAGCTGCTTTTGATACACCTCTAGTTGCCGTTCCTTTTCTTTAAATAGCTGCTGCATCTGGATAATTTCCTCATCACGTTGACCTAACTGGATTAACAGCTCACTGTTTTGTTGGCTCTGGCGTTTAATTTCCCCTTCATACCGTACTTTGAGCTGTTCTTGCAACACCAAGATCTTCTTTAAGCGATCCTTCTCTGATTGCTTTTCGGGGAGAAATTTCTGTGCTAAATACAGCAATGTTTGGTTCCAGTCAAGGTGTGCAGGGTTGGTTTGGCGTAACTGCACATAAGCCTGACGCGCTTGCTCTAGGAAGTCCTGGAGCTGTTTTTGACTCGCTAGTGGAAACTTTGAAAGAAAGAAGTTAATTGTCTCTCTTCTTTGAAAGTCATCAAAATCACGGTAATACTCAAAGTTAGTCATTTAAGGCCTTTTCACGGAAAAAGATAATTACAGCATCAGGATTAGTAAAGTCCAAGTCACAGTGGTACTTGACATACTGCCCAACACTGTTAAAGATTGCTAACACAAAACTAGTAAACAAAAAGAAGGGGATTTTGCTCGAATAACACGCATAAAAGTGCTTAATCTTAGCACGCATAGTACTTTTAAAAGTTTCTACATCGCTAAAATCACGCGGATTGAGCAAATAATCCTTGACATCACTAACCCGTTCCACCACCCAGTCATACGGAAACTGGTTAGGGTCAAAGTCGTGTTGGATGCGTTGAAAGTCAGCTGCGTCAATTTGGTCTAAAGTTTCAATAAAACCGTTAATGTATTTCTCAAAGAAGTTACTCATCCTTTTTGTTATCAAAGATAACTTCGAGCATTTGTTGAGGTGTTAAGGTACCAAAGTACTCCTTAAAGTTATCAATGTTACCCAATATTTTAGCAAAGATTTGGTAATCGTATTTTTGTCCTACCAACTGCTGGGTAACGGGGGTAATGTCGATAACACTCAAAAAGTCCCCATAGAACTCAATGTCGACTACTTTACCCTGATCTACCTGCACGTTACATTCAAAGATGCCCGCACCCTCAAACCGTTTCTTGTTTTTAAAGTTGTAGTCAGCTGTTTTACCAAAGTTTCAATCCCAGGATTGGAAGTGTTCCTGTGCACGTTTTTCCACCATCTTAATGCTGCTTTCATCCAAATGAATCGTTTCAGCATGTTCGGTTTCGGTAAAGAACTGCACCATCTTTTCCAAGAAGGTTGGGGTAGAGAGGTTGGGGAGGTATTCTTTTACGTTAACTACCCGTTTTTGGACACTTTCAATTCCCTTACTTACCATTTTTGTTTTATCCACATTGAGGTACTTCGCTAGCTTGGTAAAGTCGGTATCAAACAACAACGTACCGTGGACCAACACGCGGTCTTTGGAGAGGTATTCCGCTAAACCAGAGAACTTCTTGCCCTCAATTTCCAAGTCATTGCGGCCATGAAACTGTGCGGGTACACCTACACTGTTTAAGAACTTCACCACATTACGGGTTGTTTCTTCATAAGCGTTTTCCATTTCCTTTTTTGCTTTGGGCAAAATAATTGAGAAACAGATGTTACCCATGTCATGAAACACCGTCCCACCTCCAGAAAAACGGCGAAACAAATTAACCTTGTCCTTTTCCACTTCACTAAGGTTAACCTCAGCGTACGTATTTTGGTTGCGCCCCACCACAATCGTGTTGGCGTTTTGCCAGAAGTAAATGACTTTAATTTCCTCGCCCTTTTTAAATTCGGTGAGTAGCCATTCTTCTAACGCTGCGTTGAAGTAAGGAATGTTTTTAGGGGAAGTGAGAATGTAAGTTTTCATATTCAGTTGTTGATTAGTTAGACAGTTTATTTAAAGTGATCAAAAAGAGCTTTTTTGCACACATCAGCGATCATTTCGTTGATGGTAGGATGTGGTGAAATGGAGTTGGCAATGTCAAATACTGTCAAACCTGCATCCATAGCCAACGCTAGCTCGGCAATCATGTCACTAGCAGTAGCAGCAATAATACAGCAACCCAAGATCTTACCGGTTTGTGGGTCAAACATCATTTTGACAAAACCGTTGGTTTCATTGTCAGCGATTGCTTTTCCACAGTGCGCTAAAACAAGATTGGTTTTGACATAAGGAATGCCCTGTTTTTTCAACTCCATTTCGGTGTAACCCACCGATGCTACTTCCGGATTGGTGTAAATACAGGAAGGGCAAGTTAACTTTTGCGCTGGTTTGACCTGCTTCTTGTTTAAGATATGGTTCACCGCATACCGCCCTTGTTGGTAGGCAAAGTGGGCCAACATTAACTGGGCATTGGCATCGCCAACTATGTAGATGTTGGGAATTGAGGTTTGGAGATCTTGATTGAGCACAATCCGGTTGCGCTCATCACGTTGTAAGTTCAACCCATCAAGACATTCCGTGTTGGGAATGCGACCAATCGAGACCAAAATGCGATCACCAACCACACTGCCTTCCTGCCCGTTTTGCGAATAAAAGACTTCATTATTGTTAGCACGGGTTACCTGTGCGTTGGTAATAATCTTGACATTCTTGGTTTGCAAGAGTTTAGCGACTAAATCTGATACCTCAGTGTCAAAGATTTCCAAGATCCTATCCACCCCTTGTAAGATAGTCACTTCACTACCCAATGAAGCGTAGAGAAAGGCAAATTCAATACCAATCACTCCACCACCAACAACAACTAACTTGCGTGGTACCCCTTCCAAAGAGAGCGCTTGGGTCGAGTCAATTACAAAACCGTTTTGACGCGCTTCTGCAAAACCCGGAAGCGTTAAGTAACGGGGTCGTGAACCAGTTGCGACCACAATGCTTTTGGTGGTGTAAGTTTTACCCGCTACCTCCACGGTGTTGGGATCCAAAACCTTGGCTTCTCCCATTACCGTTTCAGCTTTAGCGGAAGCAATAATCGCTTTAACCCCACCAACTAACTTACTCACTACCTTACCTTTTTGCTCCAGCAATTGGTTTCAGTTAAGTGCTACTTGACCATTAATTGAAATACCATAGTCCTGCGCATGGCGGAGGTAATCGACAATCTTGGCCCGTTTTAACAGTGTTTTCGTTGGAATACAACCAACATTTAAACACACGCCACCAAAGTATTCCTTTTCGACAACGAGTGTTTTAAGTTTATGCTTACCCGCGTACTCAGCGGCCACATACCCAGCGGGACCAGCCCCTATAATAATGAGATCGTAATTCATCTAAGCTACTTCTAAATCAATTAATTCTTCGATTTGCTTGGCAATTTCCTTACCAAAACGACCCACATCAGCGCCATCGACCCAGCGGTGGTCGGCAGCAATTGTTAACGGTAGGATGGTATGAACGGCTACCCCACCTTCAGCACGCACAACACGTTCTTCCATGTTACCGGTAGCGACAATACACATTTCCGGGTGCTTAATAATCGGGGTACCAAAAGCAGCGCCTAAAGAACCAAAGTTAGTTACCGAGATCGTTCCCTTACTTAAATCAGGAAGCTTAATTTGCTTACTGCGGGCACGGTTAGCAAGATCAACAATGTCCTTGGCAATGTCGACTACCGACTTCGTTTGTGCTTGCTTAATGTTAGGCACAATCAGACCATCGGGAGTGTCCACGGCAATACCTACGTTAACATCCTTGTTTAACACAATTAGGTTGCGTTCCTTGTCATAACGACCATTGAAAACAGGAAACTTCTTCAAAGCATTAACAATTGCTTTAACAAAGAAAGCAAAGAAGGAAATCTTCATGTTGTACTTGCTTAAAGCTAAGCCGTTAACACTCTCACGGTACTGCTTTAACTTAGTAGCGTTAACATAGAAGGTGAGAATCGTAGCGGGAATGTTTTCGTGTGATTTCACCATTGCTTCAGCAATTGCTTTACGCATTGTCGTGATCGCAATCGTTTCCTCACCACTAGGTGCTGCTGCAGATGCTGGAGCTGGAGTGGGAGTTGGAGCGGATGTAGGTTGTACTACAGGAGCTGGTGTTGGTTGGGGCGCACTTGGCTGCACACCAAAAATAGGAAACACACTGTTCGATACCTTAATTTCACCCACTACCGAGGCACCCGCCTCTTCCACTACTGGTTCGGTGGTTACAGGTGCTGGTGTTGGGGTAAATGTTGGGGTTGGGGCAGGAGCTGGTGCAGATACTGGTTGGGGCGCAGCAGGTGCAGCAGCACCAGCGCCATCATCAATAACGGCCATCACCTGACCAATGTGCACCACATCACCAACATTAGTGGTAATGGCGGTAATAACCCCAGCATAAGGGGATGGAAGTTCGGTGGTTACCTTATCGGTTTCCACTACAAAAAGGGCTTCATCGACCTTGATCGTATCGCCCACTTTTTTGAGGATTTCAGTTACTTTACCCTCATGTAAGCCCTCACCAACATCGGTGAACTTAAACTCATTTGCCATGATATAAAAACTGTTTTAATTTTAGATTTAAAGCAAAAGTAATGCAAATAAAAAACCAGTCTTACGACTGGCTTTTTATCTAAAACGAAATTTAGAGAAATTTCGCCTTATTTTTGGCAGCATTTGCAACCGCCACAGTTGCATTTACTGCAGTTAGCTGTTTCGGTTTTGCAACCACAGCATTTCTTGTCTTCCATCTTAATATGAAATAAATGGTGTTCCCTAGCCTTCTCAACTTTTAGTTAAAAAAACTAGGTGCAATTATTCTAACAAACCTCAAACTTATTTCAAATGGCTTGATTTAGAAAAGTCGTTAGTTTACTTTAAAAGTTGGTTAACAGCTTCCAAAATGCGGGCATTTAAATTGAACTGGTAGTGTTCACCCCGTGCTAACGGTACCACAATGTCCCAACCGGTTACCCGTTGTGGGGCTGCCTTGAGATAGCTAAATAATTCTTCAGTTACCGATGCAATAATTTCCCCACTGGTGGTAAAGGTTTTAGCCGCTTCGGTAACTACGAGCAAGCGCCCGGTTTTCTTCACGGAGTTAAACACCGTTTCCTTGTCTCAAGGGGAAATGGTACGCAAGTCAATGAGTTCAATGCCCTTGTCCTTCAACTCACCCCCATAGACCATGTTAATTAGGTCAAACATGGTAGGACCGTAACTAACAATAGTAAGGTTGTTACCCTGGCTAATTAAGTTAGCTTGACCAATTGGCACAGTGTAATAATCTGCTGGAATTTCCTGACGGAAGGCACGGTACAGCTTCTTTGGTTCAAAGAAGACCACAGGGTCAGGTGATTCCACTGCTGCTAAGAATAAGCCCTTGGTGTCGTAAGGGTTGGACGGCATCACTGTTTTAAGTCCGGCAATTTGACCGTAAATCGCTTCCAAGGTCTCACTGTGGTGTTCAAGTGCTTTAATCCCACCACCCATTGGCATGCGGACAATAATCGGACAGGTATACACACCGCGAGAACGGTTGCGGATCCGTGCTGCGTGGGTAAAAATTTGGAACATTGCGGGGAAAGAAAAACCGGAGAACTGGATTTCCACGATTGGTTTAAGTCCACCAATGGCAGCGCCCACACCAATACCAGCCATAGCTGCTTCGGCAATCGGACAGTCCCAAACACGCTCCTCACCGTACTTCTTTTGTAAACCCTTAGTAGCACGGAACACACCACCTTCAAAGCCAGCATCCTGACCGTATAAAACAACGTTGGGGTCGCGCTCTAACGCTAGATCCATTGCGTTACCCAACGCTTCAATGTTATTTGCTTGAATTGTTTTTGACATAGTGAATTTATTAATGTGAAATTAGTCTTTAAAGTATTTTTTAGCTTCAGCCTTTTGGCGCGCTAGATCAGGAGTCAACTTTTCATAGTTGTAATCAAACACTTCATCCAAGGTAACTGGCGTTTTGCTAACCATTACCTCATATGCAGCTTGCACTTCTTGTTCAATCTTAGCTACCATTTCCTCTTCTTGTTGTGGGGTTAGAATACCACGGTCAAACAAGAAGTTACGTAACCGCTTCACAGGGTCACTCTTCATCGCTTCCGCTTCCTCTTCTTTAGTACGGTAAATGGAAGGGTCATCGGAAGTGGTGTGGGGACCTTGACGTCAAGAGAAGAACTCAATTAACACTGGTCCATTACCGCTTCTCGCATAGTTAGCTGCTTCGTGCATTGCTTCGTAACTCGCAATTAAGTCATTACCGTCAACGCGAATACGAGGAATGTTAACCGCAATAGCCTTGGTGGAAAGGTCACTTACAGCGGACTCTAATTTAGTTCTGGTGGAAATGGCAAACTGGTTGTTGTTAATACAAAAGACCGAGTTTCACTTGTGAATGGAAGCAATGTTCATCGCTTCATAAAACTCACCTTCAGCTGTACCACCATCCCCAATCATCGTAACGGCTACGTTAGGGAGTTTCTTGTAGTGCAACATGTAACCAAGTCCAGCAGCATGAGAGTACTGTGCACCAATGGTAATGTTAATGGGCAGCGTTTTGTATTTAGCTTCGATCTTACTACCGTTTTCGTTACCGTTTCAGTAAAGTAAAAGCTGTTCTGGTTTAACACCACGGTACAACATTAACGCACCGGAACGGAAGGTAGGGCAGAACCAGTCATTTTCATTTAAACCCATGCCCATACCAACTTGCAGTGCTTCCTCACCTAAGTTAGGCGCAAAGTTCAACATCTTACCAGCACGTTGTCAAACAAGCATCTTCTTGTCCATGATTCGGGAAAGATTCATGAGGTAAAAAGCGTGCTTTAACTGTTCGTTGCTAAGTGTAATCTTGTGATTAGGGTCCATTAACTTACCCTCATTGTCATACACTTGGTAAAGTGTAGTAGGGACTTTGTTTTTAATCAAAATTGCCATAATTATTTGTTAGTTTAATCTTATTTATTTTTAGGGGTATAGTAACTAAAACCAAGCGCTTGCAACACGGCTGCTAACACAAAGTTGAACGGCTTATTGTAGTGGGGAAGAAAGTAAACGTCTACTAAACCCAGTTCACTGATCAACATTTTCTTCTGTACTGCTAAAGCAATATAGAAGATAATTTCACTGTGGTTGGTGTTTCACGAAAGTAATTGGGCACCCAACAAACGGAGCGTCTTCTTGTCGTAAATTAACTTAAAGCGCACCTTGTCAAAGGTCCCCATAAATTCGGGACGGTCGTTGTCATCAACAATGCTCACTCCCACATCAAAACCGTTCATTTTGGCACGTTTTTCGGTTAAACCAGTAGCTGCTAGATTTAAACCAAATACGTGCAGCGCATTGGTACCAACAATGGACTCTAACTTTACAGCCTTGCTACCAATCATGTGCATAGCAGCGACTAAACCACTTTTCACAGCGTTGGTAGCTAAGTCAATGTTTTCGTATTGTTCACTAGCTGCATCATAAATGGCAGCAGCCCCACCAATAACATACACATTTTCATGATTTAAGGCTTGGAGGTATTCATTAACCTTAATCGAACCGTTACGGTTAAACTCAAACTGCCTGTCTTTAGGGACAAACTGGGTGTTTGGGCGGAACCCAATCGATTGGATGACAAGGTCAGCATCCACCCGACCCTTATCGGTTTCAACACCTTTAACGACATTTTTGTCCGCATCAACAATAAAGCCCTTAACGGCACTACCCATCATTAGGTTAATGCCCGCTTTCTTCATGGCTTTTTCCAACTCGTTGGTAAACTCTTCATCAAAGTTATTGCCAGCTGGTTTGTCCAACATGTCAATCACAGTCACCTGTTTACCACACTGTCAAGCCGCTTCTGCTAACTCTAAACCAATGTAACCCGAGCCCACAATGGCTACTGATTTAATTGACTTGTCATGACGGAAGCTGTCAATTAAGGTAAGGGCGTGTTGGTACAACTTACAACTAATTAAGTTCTTAATATTGCCACAGTACTTATCAGTGTGGTTGAATTGCAATTGGGTGTGGGTTACTTCGTTTTCCACATTCATACAAATCGGTCAAGCTCCAGAGGCTACCACCAATTGATCATAGTGGTCCACAGTTTCCTTACCAGTGGCCAAGTCCTTCACGATTACTTGTTTTTTGTCTAGATCAAGACCAACTACATCATGAGCCATAAAGACATTAGCACCCATAGCTTTTAGCTCTTCAGGGGTGGAGTAGAACAGGTCTTCGGTGTTTTTAACTACACCACTAACTGCCAAGGCAATTCCACAACCCAAAAAGGAGATGTTGGTATTGCGATCATAAGCGTTGACCTGAAAGTCTTTGCTCTTGCTTAAAAGGGTTCTGATAAAACTGGTGCCGGCGTGATTGACACCGATAACAATCACTTTCTTCATATCGTAATCAATGTCTTAAGATTAATATTTTCACGCCAAACTAAGTTTAGTTAAGTGCGGTATTTATTTAATAGTGGAGCAAGATCTTGGTTGCCACATTTGGTTGTAGCTTTGCTTTACCGTGCAGTTTTTGCAGTTCAATTAAAAAACAATAACCCACAGTTTGACTACCTAAACGCGCTATTAGTTTGTCAATCGCTGCAACCGTACCAGCAGTAGCTAGTAAGTCATCAACAATCACACAGCGCTTGTAGTTTTCTAGTGCATCAACCCGCATTTCCAAGATTGAGTTTTGGCGGTATTCCAAATCATAGGTTTCACGCGCTAACTCACCTGATAGCTTATGGGGTTTGCGCACTAACACTAGGGGTAGTTTGGTTTTGGAAGCCAAAGCACCACCAAAGATAAAACCACGCGCTTCGGGACATACGATGCCATCAGCTTTAACTTCCTGGATAAACTGTGCCATTTGTTCCAGTACAAACTCAAACAGTTCACTGTTTAAAAAAATTGGCGTAATGTCGTAAAAGAGGATTCCCGGAGTGGGAAAGTCATTAAATCGCTTAATAGCTCGATCAAGCGCTTGGAGTTTCTGTTTCACTGTTTATTATCTTAAAAACTGTTAAAAGCGTTTCTTAAAAGCTGTTAATTCCACTAATCAACTGTTCTTCAATTTGATCCACTTCCTTAAACATTTGCTTTTTGTGGCGTAAGGTAATGTGGATTTGGACATAGTTGTACGGACTTAACCAACCATTGATTAAACCAAAGCTAAACAGCGGTAAGCCATTGACAAACACGCTAAACAAGGCCACAATCGCAATTAAGTAGAAGATCACTAGGTTAATCGTGCCGTTAATACCACCAAAGTTAAAGGCAGCGTACACAATGAAGAGTAGGATTAAAACACTGCTACCAATAAGATCTAACCTTCATTGGCTGTTAACATTGTCGTTAAAGAGGTTTTTCACAAAGACCCGGTCCTTCAAGCTGCGTTTAAACCAGTAACAGTAAGTCCAAATGGTAGTTTGACCAATGCGGGCTTGGAAGAACAGCCAGAACAGGTACACAAAGATAATTAGTTGAATTGTGGACTGGTCAATCCTAAAGATAAAGTTAGCGAGGTACATTGCCCCAAAAAGTCCAGCGGCTAACAGAATTAAAGCAATTAGGTAGCTAATCCCATAAGCGACACCATAGCGCGGTAGCGAATACAAGAGATAGAGCAAGCTAATCACCCCCACACCTAACATAATGCCAGTACTCGATTCGATGTCAGCGGCTAAAAAGTTACCTAACAGTTGTGGTGCGCCCAGATAAAGGCCGAGCATCACCACCCCAAAGGCGAGAACTGTCAACCAAATAAACAGCAAGCTCCACTTTTTCTTACCAAAGAAGTCGTGCTGGTTCTTACTGATAAAGAAGTCCGTTTGGATGTCGTTGTTTTCCGTTAAGATCGAGTAGTTACTCGATGTTTTGGTCAACGCTTGCGCGTCCTTTTTATAGAGGAAGAAGCGTCATTCTAACCCGGAATGGGATGACACAAACAACCACACTAAGATTACTCCTAAACCATAGTTTAAGAAGTAAGAGGTAATCGCTGAAACTGCCATTAAGGTACCTAACTGTTGTACCTGGTAGTTGGAGAGGTAAATCACGACCAAGGCACTCAATAACCAGCAAATGTGGGTTTCTATCATCGTGAAGAAACTACGTTTTAAACACAGTTTTCACGATTCCACAATTGAGGCGTTGTTCTTAATGTTACGCAAAAACAGTTCCGCCAGAGTGATCAGGTTAATAATGTTAACGGCAACCACAAAGAAGATGCCGACAAACGAAAAGACATCAACGACACCACCAACGGCATTAAAGAAGGCGAGCGAAGAGATGGCACTAAGCGCTAAGGCTAAGGCTTTGTAAAGGCCAAGTAGCTTGTAACGAATACCTAACGCAATTGTAGCGAGCAGGATAATTACACCCAAGGCAATGAAGCTCGAAGCAAAGTTAGAAACATCGCGTGCTAAAGTGTTGGAAAGCGGTGCATTAACTAACTTGGTGTTAGCAGCTGCAATGGTAGCGGTTTCGGGAAAACCATTGCTCAACAGCACCTTTACCCTACTAGCTTCCAAGTAAGAAGCAGGAACGTATGAACCATCATTGTTAACAAAGTTAGCTACTTGGAACTTAATGTTACCACCGTGTTCTAAGCGTTGGTTATTGCTATCCAAAAAGCTTTGTAAAGTGGCGTTTACCTTAACCGGATCACCATTCTTAGCTACCGGATCTTCTGTTCTAATTTCTGCCAAGATGTAATTGGAGAATAAACCAGAAAAACCAGTAGCATCACCGCTGCGCAGTTTTCTAGTGTCCGCGCTGTTAGCTACAGTGACAACATCAGCAGCGTCCTTATTGTTATTGTCTTTGGTTGCAACTTGTGAGAAATGTGTCGGTGCTGCATAGATGTAATAGATATTTTTTAAGGTAATGTCACTAGCTTTGGTAATTGGTGAATTACCATTATTATTTTTTTGCAAAAACAAATTATCACCAGAGGAAAAGTTACCCTCAGCGTGCAAGAAGTCCCAGAGGTTCTTTTCACGTTCGTTAAAGGTGAGGTAATCAGTACCACCTTTAGGAACGTTAAAGATGTTGCGCACATACTGTACTGCACCTTCCTTATCCTTCCATAAAACTAAAGTTTTATTTCCGTTTTGTGCACTACCGTTAGCCTTGTCTTTAAATCATTTACCGCTACCTTCAACACTCTTTTGACCATTACCAGTATTATAGTCAGAATCAGTTAAACTCTTACTCTTAATAAAGTCCAAAAAGTCGTTGTGTAAAGATTCTTTGGTAATAACATTTTTGCTGAGCTTGTACTCAAACGTTAAGGCACTGTTGGTGTTTGTGGTTTGGGGAACAACTGCTGTAAGCGAATTTCTAATAAATTCCTTATCAAACGAGTCAAAAAGAACAATTCCGTCAGTGGTTTCTAACGAAAGTTTGTAGTTGTGTTCAATTGCACTTAAAGCGTTGCGCTTGACCTGTGCATCTATTTGCCGCTTTCGTGCTCGGGTACGCGCCTCTTCACGACGTTGCTCAAAGGTAACAGAAATAGAGGGTAAACCATCATTAACTAGTCAAGAACTGTCAAAAACTCCTTTATCTGAAAGTTCTTTTGCACTTGTATCGTGTGAGTTTTTCGTGATGTTAACAGACGAAAAACCCTGGGCGTAGAGGCTGTTGGCGTAACTCTTTTCAATATTGTTGAGAAAACCGTTCACATCGGTAATCATTTCAGTATTGTTACCGTTGCCATTGCCGTCCTTTTTCTGGGTAGGATCTAACTCCTTGTTAGCGTCCTTGGACTTATGGTTTAAAAAATAAACAGTGGTGGTAGTGGAACCGTTGAACACTACCCCTAAGCGACTGTCGTTTAGAAGCTTATAACTACCAAAAATAATTCCAAACAGGCCCAAAAAAGCTAAGACCAGGATAATTCCAATCTTTAAGAGCCAGTCAAAGGAAACCCGCTTTTTCAGTCCCACTGGATGTTTTACAATTGACTTAAATTATATAAATTTAGCAAAATGGCCACTTTAGTTGAAATTGAACGCGATTTTTTACAAAAAACAGCCCAAAAATTTGCACCTGAAGTGGTTGCGTTGATTACTAAAGCACTCGATTACTCCAAAAAGTGACACGGTGAGCAAAAAAGGCTGAGTGGTGAACCGTTTTTTATCCATCCCTTGAGAACAGCACTGCGCTTAGTGGAGTGAAACATGGACAGCAACACCGTGTGTGCTGGTCTGTTACACGACATTATTGAAGATACCCAGGTCACTGAAGCTGATTTAACTGCCATTTTTGGTAAAGAAATTACTGATTTAGTGGTAAAAGTTACCAAAATTACCAGTGAATCCAAAAAACAACGCCAGCTAAACCGAAAGAAAGAAGATTTAAACCTCAAAAGTTTGGTTAACATTGCGATGAGCTCGCAGCAAGAGGTCAATGCTTTGGTGTTAAAGTTGGCCGACCGACTCGACAACATCTCCTCAATTGAATTTTTAGCAGTCGAAAAACAAAAGATTATTGCTAAAGAAACACTCGAACTGTATGCCAAGATAGCCGGTCGAATTGGAATGTATCCCGTTAAAACTCAACTAGCCGATCTTTCCTTTAAGGTATTAGATCCAAAAAACTTTAACAATACGCTGTCCAAGATTAACCAGCAAAAGGTCTTTTATGACAACGAATGGGACAACTTTAAAAAACAGTTGGAAGAGATGCTGGAACAAAACCAGATTGAATACCGCTTAGAAAGTCGAATTAAAGGCATTTATTCTACTTATCAAAAATTAACCTTTCACGAGCAGAACATTGCCAAAATCCACGATCTGTTCGCCATTCGTTTAATTGTGAAGTCAGAGTTGGATTGTTACCACTTACTTGGTTTAATCCACCTCAACTTTACCGTGTTAATGAAACACTTTAAGGATTACATAGCATCCCCCAAACAAAACTTTTACCAATCAATCCACACGACTGTGCGCTTGAAGGGTTTAAATGTGGAAATCCAGATTAGAACCCAACGAATGGATCATGTGAGCAAGTACGGTTTTGCTAGCCACTGGATTTACAAAGAGAAAAAGGAAGGGTTATTGGCAAGTGCTTTACAAGTAAATTACTTAAACAGTAAACAAATGCATTCGCGCGATTTTTTTAAACGAATCTTTGGTACTGATATTATTAAGGTTAATGTAAGTTCCGATAACGAACCTAACATAGTCAAAAAACTCAATGTTGAAAGTAACAGCAAGTTGCTCGATATTGCTTATGAGTTGTATCCCAAACAGTTCAATAAATTAGAAAAAATCAAACTCGATGGGGTTGAGGTTATGTCGTTTGACGTAACTGCCGAAAACGAAATGGTAATTGAGTTTTGTTTTGGTAAAACCAATAACCTCAAACGGCGTTGGTTACGTTATATGAATAACCCCGTATTTCGCGAACGGGTTAAAAAGGATTTAAACAAGTTAAAGAAAGCGGTTAAGTACAGTGAACTACCTCTATACGAAAAGGCCTTGGAAGAGTTGCACTTAAAGCTAGCTGATGAAACCCAGATTAAACAACGCTTAAATGCTTTAGGGATCAAAAAACTGACAGAGTTCTTGGAATTAATCGAATACCCCCACTTCCCTAAAAATGAGCATTTGTACTTTTTAGCTAGTAACAATCAGAAGTGAAGGGAATTGATTAAACCCATTAAGTTCGCTTTATCACAAGCTGTGTTCCAAAACTCTTACTTTGAACAAATTGAGGGAATTTACATCACCAAAATTGTGATTGAAACCTGTTGCACAAAGATTCCTGACATGCCAGAACAAGTTATTGGCATCTTAATGAAAAACATTCTCAGGGTACACCTTCATGATTGTAGGGAATTAGCTAATCAAAAGCAACCCAAAATTATTCCACTTTATTGAAATGCACACCAATTAAAGATGCGACCACGTAAATTCCGCTGTCAAATTAACATTCGCGGTGTATGGAGTGAAACAACGGTCAATAAAATTGTTCAAACCATTATTGAAGGTGATAGCTATTTAGAACGAATTATTCCCAAGATCGATAAACAAAAAGATGAGTTTGAGCTCAACATTACTATGTTTATCGACAACTATCACCAACTAATCACAATTATGGAGCAGATTACTACTAAGAATATTAGCTACGTTTGAAAGTACCTTTAACTTTCAAATACTTCGTCATAAACATCTTGGTTCTTTTGTAAAAGTAACACTAAGTGTTTCTTCAATAGCCTTTGTGGGTTAAAGCGCGATACTCGTACCCCATAAACGCGTTTGGTGAAAATAAACAATAAGGCACCAGAAACAACAAATGTACCAATAATAGATACCCCGACATAAAAGACAATTTGTGAAATATTAGTTACGTATTCCTTGAACTGATCATTTTCGTTAGGATTTGGAAAACGTTTTAAGATTTCGTCCACGTATTGATTAATCTTTTCAATTGTGTTCTTAACTTCAGTAAAAGTAGATCCTTTTGAGAAGTTGTTAACCTTGCTTAATGAATCTTCGAGTTGGTTAAAACTGCTTTCTAAAGCACTGTCATCCAGTAATTTTGTCAATTCTTTTCCTTCTTTAATTGAACTTTCAACATCACCTAACGTTTTTACAAGTTCGTTACTAGAAAGTGACTTTGAAACTTTTTTAATTTCCTCGTTATATTGTTTAACCTTGTTATAACCTTCCTTGTAATAGTTATTAATAGGTTCACGGTTTTCAAAGGCAAAGTAACCCGCTGCTGTAATGCCACCAACAAGTAGAATACCAATCAAAACAATTAGGAAAGTGGAAAGCTTTTTTAAAAACTTAAACATTATTGATCAAGCTCCTCTAAAACAGCATCTTCCAACTCTGGATAACGATCTAAGATGTCATTAACATGATCAGCAATTTGATCAGTACTGGTTAAACGCGAAAACGTTCTAAGTCCTAACTTTTTCGAACCTAAGAAGGTCATTACAGTAGAGACTATTAGCACTGCTAAAACGCCACCAGAAACACTAACTAAGGTAATTGCGCCTATGGTGTAATATTCATTAAACTGTTTTTCTGTTGGAATTTTGTTTTTAATTTCTTTGTACTGTTTAATGATGTCATTTGCTTGTGTAGAGAACTCTTGAATATCTTTCAAAGCACTATTGGTTTTACCGTTAGTATCTCTGGTACTTACACTAATTGTTGAACCAGAAATCTTAGTAATTGGAATAGTCGAAGAAGATGTGCCATTTTGACCAGTTTTACCATTATTAGCGGTTTTTAAAGTATTTGCATACTTTTCTATTTGACTCTTAGTAGCTTGTAGACTCTTCTTAGCTTCTTGTAACTCCTTTAGTTTTTCCGGACTCACAAGATAGCCGTTTAAGCCTTTAGCTTGGTTTATTTGCTTTTCGTACTCCTCTAACTTTTTAGTTGATTCTTCAACTTTCTTCTTAACCTCTTCTATTTGTTTATTAATGGATTCAACACTAAACTTACTGGAACTTCCATTATAAGTATCTATAAACGCTTGAGAAGAGTCAAAGGAACTGTCGATCGATTTGCGGGTATCAATGTAAAGTTTAGCTGCGTTGTCTTTAAAGTAAAGTGCAGCACCGCCAAGACCTAACAAAACCAAAAAGACAAGCATCCCGAAGAACAGAATGATTGTTTTGGCAAAGCGATTAATAAAAAGGAGCATTTAAAAGCTTAAGTTAGGCTTAAGGTTAATATATTTAACCAAACGGACAACCCGCTAAAGGTTAAGTTCGAATCTCTTGGAAAACATTTTCCCGATCCAATTTTTCTTTCAATTCCGCTACAACGGTTTCATCTTTAGAAACATTAGTAATATTGAGAATTTCGGCGTTATCACGTAATACACCGATTAAATCAGTAACTGATTTGCTCATATCAGTGACACTACCAATAACTGGTTGGAACTTATCGGTACTCTTTAATGCCACTTGTTCTTGTGGACTTTCAAACTTCACGGTGTACTTTAAACCCGGACGGAGGTTTTTCACTCCATATTCAAAGACAACCCCAATAAGTTCAGCGCGTTGTACAATTTCCTTTTGGAACTCACTATCACGGGTAGCGCGTTCCGCTTCGTAAGCACTGGCACCCGGATAGTAATTTTCGTTCACCCGGTACATCGTGTTGCCGCCATGAGTGTAAGTTTCCTCTAAGTGGAGATTAATGTCTTTTAGAGCAGCGTAAACGGTGTCGTTGTCTTTATTGACATTTTTTTCATTAGTGTTTTTCACCAAATAACGGTCAATGTAACCACCAGCTCAGTAACCTTGCATTTGTCGTTCAAAACGACGACGAATGTAAATGTCACTAACAGCACGCTTTAACTTAGATGCATCAATATTGTCTTGGTTATCTTTAAGTTCTAAACTAGTTAAATCAATGTAATTAAACGGCTTGGAAGCAAAAAGGTCATAGATAATATTAGTGTGATCGCCTAAAACTAGTGGATTAAAACCAAAGTTATTGTCTTGGTTAGTAGAGTAAAACTCCAACTCTAACACGTTTTTAAAAATTTGCTTAGCAACATTAATCGAGTGGTAGTCACCATAAATTGACACCTTTTTAACGCTTTCTGGTAATTCCAAATCCTTTAAGGTAGAACCATCACCAGTTAAACCATTAATGATGACGTAATCAGGATTACGTTGTTTTAAATTGAGGTTAATGCCGTCAGTATTACCTTGCTTAATCCTTGGAGCATCAAAGGCAATCGTTTTTATTTGCTTAAAGTGATGGTTTGGGTAAACGTTGTATAACGGTGAACCGTTAGCAACATAGTAATTCCACTCTTTAAACAAGTACTCGCTTTGTCCATTGGAGCCAGCATAGTAAGCCTTATCCACGTCATGGAACCAACCAGGGAACTTAGGGACACCTTGATCGACAAAGATATCATCGTAAGCCCTATTGTTAGCAGTTAAAGTGCCGTTGGTAGTTTGCAAAATTGGCATTACTACCCGGTTGTTAAAGTATTCGGTTGATTGGTAATATTTGAGCGTTTTCCCGTCCGCTGCTACTTGGTCGTAGTAACCCGCTTTAAAGTTTGGCGTGATAAAGGTAGAACTGGTTTTAATCAAACCGCTCACCTCTACCTTAGGCACTGGTCGAAGTAAGTCTTTTGGTGCATCAGTAATTGCTTCTGATAAGTTATTGGCAAAATTACGGAAATCCTCACGGAAGTTGGAACCATCACCAAGATCGATTTCACTGCGGTATCCCCCATCGGTTAACGAAATCGAATTAAGTTTAGACTTGGCGTTTTGGTTTACCTTGGAATAAAGCTGGAAACTCAGGTTACCACTGCGCCCCGAAGCAATGAGTGTTTCACCACTTTGTAGCCTTAATACCTCATTAACGATTAGAACTGCGGAAAAAGCATTTATTGCAGCACCAAAGAGACCAAGCGCTCAGAAACCACCCTTTTTGAGGCGCTTTAGCGTCTTTTTGTCCTTGATTTTGAAATTTAATTTCATCTTTTTTAATAGTTTAAAATTGCAGAACTAAAACAGTTCTTACTCAACTAAATACTTGAACTACCTCAACGACAACGGGAAATTTAATTGAACACCTATTAAAGATATAGTGTTCTTCTCTATTTTAGATTTAGTAATCCCTAAAGGAGAAAATTTACGCCAAATGTGTACCAAAAAAAGAGAAATTTTTGGCGATTACGTTATTCATTTCTTTCCAAAATAGAGGTTAAGCGATAATAAAAGCACCCGCTAAGCAGGTGCTAACCTTCTTTTGGAAATGTTTAAACTGGAGCGAGTAATCGGAATCGAACCGACACGATCAGCTTGGAAGGCTGATGTTCTACCACTAAACTATACTCGCAGAACTTGGTGCCCAAAGCCGGACTTGAACCGGCACGCTATTGCTAGCGTGGGATCTTAAATCCCATGCGTCTACCATTCCGCCACTTGGGCTGGTGACTCACCCGAGGATCGAACTCGGGACCCTTTGATTAAAAGTCAAATGCTCTACCGCTGAGCTAGTGAGTCAATAAACAGGGTTGCCCCTGTCATTTTATGAATGCTTTTTTACTGGCTGGGATGGATGGGATCGAACCATCGCATGATAGAGTCAAAGTCTATTGCCTTACCGCTTGGCTACATCCCAATAAATGGTGGACAGGGAGAGATTCGAACTCCCGAAACCATGAAGGTGTCTGATCTACAGTCAGAAGCGTTTAGCCACTTCGCTACCTGTCCAATAACCAGCAAAAGATATTTTACAAAAATTATCCGTTAATGATCTTAACAATTTTGACCCGGTAGGGCACTTCTATGCCAACTACGTCACATTCATCATCCACTAAACGACCATAAATGGCGTGTGCCAAAGGGGATTCATTGGAGATGCGGTGTTCTTCGGGATTAGCTTCTAAAGCTCCCACAATGGTGTACTTTTCGTGTGTTTTCGAACTGTAATCGTAAATTTCGACCGTACTACCCAGGGTAACCTTATTAGCCTTTTTTGTTTGCGACTCATTAATTAACTTAACGTTGGCTAAAATGTCTTGGATTTCAGCAATGCGGGTTTCAATTTCACCCTGCTGTGCTTTTGCTGCATCGTAATCAGCGTTTTCACTCAAGTCACCCTGGTCACGGGCTTCTTGCAATAACTTAATAATAGCGGGACGTTTTACTTGAATGAGGTGTTCCAGCTCCGCTTCTAATTGTTTTAGCCCCTCTTCGGTAAGGTAATTTTTGTTAAGTTCCATTAATTAGAAATTGCGTTTAAAGTTAAATTAAATCTACTTTTTTTTCCCAATATTTGCCAAAAAAATGCAAAATTTTATTTGCAATTTGACCTAACAGCACTAGTTAAAGCTACTGCTTGTTGGATGACAGATGACAACGAAAGACAATTCTGGCATTAAAAGTAAAAGACGGATTCGTTTTTTAGCTTTAGGAATTATTATTTTGATTCTTGTAATTGCTGTAATCACCATTTTATTTGTCACGGGTGTAATTACCCGCGCTAACCAATAAAACGTAGTTGATGGCCAATAAAGATCAAAATCTAACGCTTTGATACGACCAACTACTGAGCAAAGCACAGTTAGTGAGCTATGGCGATGTGAAGGGCACTAACTGTTTTTTACCCAACAGTTGGAACCTCTGACTACAAATTCAAAGACTCTATAATAACGCTACTGCTCTAATTAAATTAAAGGACAAGGTTATTTTAAAGCAGTTTATCCCGATTGAACCACTGCCATACACAGTGGAGCAAGTTCAACTACCGACCCTTTCCTTTTACAGTGAGTACCAAAAGGAAAAGCGTCATGTGGAGGGCTTTAATCCTGAGCTTTTTTTAATAGAGCAAATAGGTACTAAAAAACTCCATGATCCGCTAGTACTACGTCCTACTAGTGAAATTGCTTTTTGTAACTTATGGAAAAAACAGAGCTTTTCTTACCAAAACCTTCCCGTTATCTATAACCAGTGAACCTGTGTTTTTCGCGCTGAAAAAAACACCCGCCCTTTCTTGCGCAACAGTGAGTTTTACTGACAAGAAACCCACGGTTTGTTTAGTGATGGAGTTAATTCTGAAAGCGCAGCAATTGCTTTTTGAAAACTGTACCAGGACATTATTGTCAACCAGCTCTGTATCCCCGCTTTTGTGGGGTTAAAGAGTCCCAATGAGCGCTTTGCCGGAGCACAAAATACCTGAACGGTGGAAAGCATTATGCCCGATGGGCAGGCATTGCAGTGTGCCACGAGCCATGATTTAGGGCAAACCTTTACCAAACCGTTTGGGCTAACCTTTCAAAACCAAGCCAACCAACAGGCCATTCCCTACAGCTTTAGTTGTGGAATTTCTACAAGAATCCTCGGTGCTTTACTGTTAACCCACAGTAATGACTTTGGACTGATACTACCATGAAAAGTAGCACCAATTCAAGTCAAACTGTACTTATTTGACAAAAAAGGTGATACAAAGACAGTAGAGTTAGCACAGAAAGTGCAAACGTTGTTGGAACAATTAGCAATCCGTTTTCAATTTATTAAGGTCGAAAACCAGCTCGGTAAACAGTTAGGACAGGGTGAAGTCAACGGCATTCCCTTTCAACTAATAGTTGACAATCCCCAAACAGTCAACATCTTTAACCGGTTAACCAGGGTCAAAACAGCTTACAGTTTCGAACAGTTGGCAAGCCGATTTGTGGAGCTCGTGCAACAGTACCATCAAGCAATGTATGACAAAGCCAAAGCAGTGGTACAGCAAAAGGTGGTGCAAGCCACTACTTTAAAGCAAATTGAACAAGCCTTTAATGACAAAAAGGCTGTTTTGTGTGCGGTTCGTTTAACCGATACATTAGAACAGCAATTAAAGGAACGGTACCAGGTCACGGTGCGCTGTTGCTTAGAGCAGCTTCAAAAGCCCCAAATTTGTCCGTTTAGCGGTGAGTCAGCACAGGACTATGTCCTGATTGCGCGTGCCTATTAATAACTTTTTACGAAAACTTTTTGCTTAAAAGCAAACCCTAAATGTAAAGGCTAAAAAGGTGGTTTTTATTTTCCACTTCTGGTCGTTTTTTGTCAATAAAATCAGTTAAACCTTTAAATTTTTTAAGGTTTTTTTGATCTATTTATGAGTTTTAAAGTTAAAAACCAGAGTAAGCACCTATACTCTTTAATGACGAAATTTAAACGTTCGCAACTGATCTTGAAGCACCAAAGCAATAACTTTGCCTCAGAGTTGTGGAACGAAGAGGACATTATCCGCTCCAAGCAGTTTATCGAACTTATAGAAGACACGCTGCTCCACCTCAAGAAGGATACGGTCGACTTTATTTACGACATCTTCATTTATGGGAAGAAGCCGTGTGATATTAGTTACTCCAATTCGACCTATTACAAAAAGTTAAACAAGGCTGCAAATAGTTTTTTTGACCATTTTGTCTGGGAGGCTCCAATTTATAAGACAAAGGAGCTTAAAAATGACAATTCTCACAGTTAGACAAATTCAAAATAATTACTATACGGAATACTACATGGGTCCGGTTAGAAACTTTGCTGACAAAAAGGAAGTTTACTTTGATGCTAAGGTAAACAATATTGAAAGCAAAGTCAACAAGGATAAAGCCTTGTTACACATAACCCTGGCGTTAAAGTACGACAGTAACTTTCCCAGTAATATGTTCCAAGCTCAGTTTAAACTGGGTAATTGAAGTAGTGAAAAGATTCCGCTGCAAAAAGCACCGGATAAAAATCCGATCTGGAAAATAAGATTCACTATTTTTATGCCACTTTGGAAGTGCCACGAACTGCCCTTATTAAACGGGAAATTAACCGCATTAGTGAGTATATTCGTGAAGAATTAACAATTGCTTTTCGCTTAAATGACAGTAGTGATCGCTACCATTGGAGTAACTATAACCTGTTCGATACAGTGGCAGCTGATATGTACCAAACGGTCATTAAAGAAACCGTTACCTTTGGTAACATGATTAGACTAAACCAACTCGAGGGCGAGAAGGAAGGTAACATCCAACAAAGTGAACTTAAATATGGTTGAACGATGTTGGACTACCGCAATATGGGGCCGCTGGAAGAGGTTAATAATTTAATTAATATTAAGTTTAACCAACCAGTAAAAGTGGTAAGTGTTGGTGTGACCGTAAGCTATACAGCGCCTGATGGTAAACGCCAAGAGCTCAAAGATCAAGCTGAATACCAGCACACCCTCAACCCCAATGAAGAGTTCAAACTGAACTTTCCCAGACGGCTAAGCTTTAACAGGGTAACTAAAAAGTTGGACTTTGACCCCAATGGTAGTGCCGTGTTCCTACCACAGGGTGGCTTTGGTAGCTATGAGATCAAACTGGAGGCTAATGTCGGCAACCAGTTCTACACAATTGTGGCTACTAACAGCTTTGAGTACGCCCATCCGTTTGACGATCCCAAGACCAATGACTTCTTTCTGGTGCAGTACGCTCCAGTCTACAGTCTGTTTAACTTTAGCGATTTAATTCAGTAATTGACCATGATCTTCTCAATTAGTAAACGCAAGTTAATTTGTGGCTTTTCCCTAGTAGCCCTTACAATCGCTGGGATTGTTGGTGGGGTTTACTTAGTCACCAAAAACAATCAACAAACCACACCACAAACAAATCACTTTAATGTAGCTGAACCAGTCAACAAGGTCCCTAATTGGCGCAAACTAGGTCCAGAAACACAACGCGAACTGCGCGATCGTCTGTATCCCTTAGATGACACAGGTTACTTTATTTACAAGTACGGTGCCATTAGTCGCTACCTCCAAAGCCAAAAGGAACTGGATGAACTAGTCGATTACCGCACCGTGTTACCCTCCACCCAAAAGCACTTTAAGTATGATTCCTTTAACCAAAGCGTGTTGGAAAGTAAGCTGCGTAAGTGGTTAATGAAAGCCATTAAACAACACCCGTACTTTCAGCACTTTGAGTTTGATCCGGTGCTTAAAGCACAGTACAATATCAACATTCCTGCTCAAAAAATTACGGTGAATGCAGTTTGATTTTACAAAAAGGATAATGATCTCACCACGGGCAAGCCAATCCGCTATTGGGATCAGTTTGAAATTAAACTAAAGCAGTAGTAATAAATCTTTATAATTCTTATTGGGTTTACCGATGCAAGAGCGTGACATTCTTTTAAAAATTAAAGAGATCGCTAAGGCTAAAAACTTCAAAACTGAGTTAGATGAAAGTGTGTTGCAAAAACCCTTTCGCGAACTGAAAATTGACTCACTTGATATGTTTAGCGTGATTGTCAGCTTAGAAAAGGAGTTTGACATTATGTTTGAAGATGAAAAACTGATGCAGCTCAATAATCTCGCTGAGTTAATTGCTGAGGTAAAACACCTAATAAGTCAAAAAGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCATTAAAGATTTGGCCTAGTGTTGAATGACACTGGGCTTTTTTATTGCTTTGATTCAATCAATTCAAAGAAGTTGGCGTTTTCCTGATCACGGAAGGTGGTTTTCACGCGACGAATTTGCTTTACGTCAATGTTCCAGTGCTTACATTTTAATCATGGTGCAAAATCAGTTTTGTGTGTTTCTCACTGACTTCAGTTGTTTAAAACCACGGTAACTTGCTCGTTGTTGTTAGTGTTAGTAAAGGTGATGAAAACAGGTTCAAAGCCTTGTTTGTTAACCTTTAGTTTGGGTGGTACAGACTGCTTAATGTCGAGCACACCGGTTTGATCATCCACCGTTACTTAGGGCAACTCTACCACTGTTGCTGGCTTTTTGAGATAACCAAAACTCAAATAGCCCAAACTAGCAAAGGCTGAATTTTGTCAATCAAACCCAACAGTTGTTAAGATATTGCGCATGTATTCCACATTACCGTTCACTGAAAGGTGGGAAAATTTATCGGCAAAGTAAAACGACTTCTTTAAAAAATCAGTGTTAAACAGTTTGTTTTCATTAATATCAATCCCACTCACTTTAGCTAACTGTAAGAACAAACTCTTTTGGTCTAGTAAATGAACAAAGCTGCTGTAATTACTATCTTTAGGAATACCTTCGAGAAAATCGTTAAAGGTTTTTCAGTTCTTTTCAGAGCTTCGCAGTAAGCTAGATGGAGCAATGTTGGCTAAAACAACATGGGCTTGAGGACTTTGCGTATGTAACAGATCCAGTATTTGGGTAAGATTATGTTTGTATTGCTCGACTGTGTAAACCCCTTGCGCTAACTGGTTAATGTCATTAGTACCCAAATTAAGTACATAAACATCAATGTTGTTGTACTGCTTAAAGCGATGATTTTGCATGTACAACTCTAAGGAGGTGTTACTACCATTAATGGCACTGTTGATCACTACATCACGGTAACGACCAAAGTCATTTTTCAAGCTTTGTTCCAACAGTTGTGGGGCACTACTGTATCCGTCAGTACCAACACCATGAGTCATCGAATCACCAATAAACAACCAGTTGGCTTGGGTTTTATCTTTTAAGAAATCAAAGATAGTTGGTGTGTTGTCTTTTTCTTTATTAATCCAACCGTTAATTACCTGGTATGAACCTTGCCCGTCTGTCCTTGTAATTTGAAGTAGGTAGTTGAGTGGCTCATTTTCTTTTATATCAAGTGCTTTATGGATATATTTCCAGTAAAGAGATATGTCAATTTGTTTGTTAGTAATGGTACCGTTTCAACGCAAATAGCTGGTAGGACGTTTCTTAATATCTCTAGCGTTAAAGTCACTCGCAAACGAAAGCTGGTACTGCAGCCCATTAGGATTAACCTCTGTACTTTCAATTTCCAACTCTTTGGTTTTAGTCTTTCAAGTAACAGTAAAGGGATTGGAAGTAGTTACCAAATTAGCTTTAGCATTATTGAAAAAGTCCGTTTCTAAGTTGTAGTTGGGATCATCAAAATCAATCCTGTCATTAATGACATTGGTGTCATGGTAATGGCCAAAGCTGTAGTAATTTTTTACATCAAACTGGGTTTCTTCATTAATTGGTATAGCTTCTAGTGATGGTTCTGTAACTTCCACTGGCAATGGTTCAGCGCCAGTAGCTTTTAACACTTGTGCTAACTGTTTACCAATCGCAAATTCTCCTTGCAAACTTAAGCTAACTTTGTCTTCACTCAAATAATTGCCGCTAAGTCAATCACTAGGTACAAAGTCACCTTCAAAGTGATCAACTACCTTAATTTTGGACGCAAAATTACGGTTTTGATCAGTTAATTGCTTAACGGTGGCTAAAGCGACAAAGTTAAGGACACTTAACTTCTCGTTAAAAGCTGAATCATTTGTTTTTCAGTGCAATTGCAAAATGAAAAAGTTGTCCTTTTCAGCCTTTTCACCAACTTGCTTTTTAATGAGTGCGGTTAGATTATTGACAAACTCCTCGTAATAATCAACCCGCTCTGCATCCGCTTGGGAAGTGAGGTAAACTAGCGTACGTGGTTTGTAAATTGAAAGTACCCGTTCTTGAAAGCTCTCATTAATATCTTTCATAGTCTGCCCGGCAAAACCAAAGTTTGCTATCTTACCTTCAAACCGAGCACTAGCAAACACAAACTGATCGCTAAAATTAATGTCGCTGATAGTTTGTTTATCACTGTTTCATTCGCATAAAACATCAACCCGATCAACTTGTCCAGCTGGTCAGTTCGGGTTGGATCAAAAGCGCCAGTACTGGTCAAAGTAAGCGACAAAGTTACGCGCACCGTGTAGTCGAGCAAAGTCGCTAACTAATTCCTTACCACCAGTTCAAGCAAAGCTTAAGGATGGGAGTTGGTACCTAATTTTGTTGTCATGTTCTTCACCATCCCTAGCATAAAAGTCCAATACCTTGCTTTGGAATTCACTACCCCAACTCGCTATTTCTGTTTTGCTAATGTTAGTTGGTAAATTTAAGGGTTCAATCTTTTGTTGATGGGTGGAGCTCGGTAGTTGGATGTTGATATTTTTCACAAAGAGTGGTGAAACATCACTCTGAGTGCCCATTAAAAAGAGGGTAAAGAAGGCACCAACACTTACACCACAACCAAAAAAGGCTAGAGAATTGAGTCAGTATCTGTACTTTGTGCGTTTTTTCATATTGGTTTATTCCGGTTCATTAATGAGTTATTTTTATTGAATAACAGCAACTGCAACTTTACCTGTTATTTAGCTATAACTAATTTTAAAACTTTGTAATTTTGAACCACATTTTTTTGAAAGAATTGGCAATCGGGATAAATTGCTATTTTGAAAAATTCAAAATTATTAAAATTAATCCCAAATGTTTTTTGGGTTTTCAAAAACTCTCTAATAACAAATAAACAATGGTTATTAAGAAGGGTTTTTTCGCGTTATCAAGTTGCACATTAGGTCTAGGTCTTATTTTAACTGCATGTGGTGCTAGGGGTAAATTTGACCAAGTTGACGATGGTGTTATTAAGCTAGCTACTTCAATCCAAAATAAGGACAGTATCGCTGCTTTAAACACCATTTACAAGAAGTATAAGGAACGCCATCCAGGGAGTTATCCAGTTCAAAATTTTCAGGTTCCCGGTGGTTACAGTGGTTTACGGAACGACATTAGAACGCGTTTATCAGCTAAAGATGGCAACAACTTTTACAACATAGTACTCAACTATCCTGATGTTGTCTCCTCATTGGCTACAAGTGACATGCAACTAATATTGGATAACGTTGACACCAAGCTCCTTTCGAAAAATTTCTTGAGCTTTAACGAGCGAATTGGTGGTGTACGACAAAAGGGGATTTATGCAATTCCCATTTCCCTTTCCACTGAATTAATGGTGCTCAACGGACCGGTATTGCACTATATTTTGAACAGTGCTAAGAAGAAGGAAAACGGTGCTCAAATGATTAAAAGATCAGGTAGTTTTTCCACTGTTCAAAAAGGTACCATGGCAATCGACATGAATGATCAAAAAACCAAAGATCTTTGACAAAAAATCGAAAACGCTGCTAAGGCTAATGGTAAGACAACTAGCACTCAAACAAGTCCACAACCAAAAAACGCTGTTAGCTCACTCCAGCTAAAACAGGCAGCTGAAGGTACTAGCACTGATAATTCTCAGGATGCAGAAAATAGCGATAACGAAATCAAAAAGACTTGGGGTGAATACAAGGAAGAGGGTAACCACACACTGAAGGGTTACACCTTTAAGGCTAGTGTCTTTGAAAACTGAAATGAACTATTAGACTTTTCCACGCGGGTAGCCAATTCGTTCCCAGACAAAATTAAAAATCAAGCATCTAAGAAAGCTACCGAACTGCAAGGTGTCTTTGGGGTTGACAGCGTCTCTGGTGCTTTATTTTCCGCTACCTTTGCTGCTGGTGGTGGAGACTATGATAAGTTCTTCTTTAATGTAAAGAATGGTCGGGGTAATTTTAGAAATTTACTCGAAAAGGGTAGTTCTTACAATAATCTGCAAAAGGTCTTTAACGATTACAAGCAGTTAATTAGCTCTAACGGTTTGTACATTAATAAAGGTGGTGCTTATTCCAGTAACTTCTTAAAGTTTCACCAGTTAGCTTTTAGTGTGGGTTCCAGTTCTGGTTACAATTTTGCTTTTGCTGGTGAAAGCGCTAAACGCTTGGAATTTGGCCAGAAAGCAATCGAGTACCCACGTGACACTTACGAAATTAAGGCACCTACAAATAGCCAAAATGGTAATGGTACCCTTCTAGGTAGTTTTACTAAAAGTAAAAGTAATGGCAAAGAACAAAGTGGGCAAGATGAGGATAACCAGACCAGTGAAACGATTGAGCTTTACAAAAGCAGCGTTCCTAGTGGTAAAGAGGCGGGTAAAAATGCGCTTGCTATTACCAATCAACAACTAATTTCGGCTTTAGAAAATGCTGCAAAAGATAATAAAACCTCCCAACCACAAGCACGATCACTTACAGCTTCTGATCAAGTTCAAATTACACAAAGTTCAGATAAGGTTATTGGTTACATCACCACATCTAATTTGGACATTGACAACAACAATACCTTTGATGTTGGTAAGTTAAACGGTGATAAGTCAACCAGCAAAATCATTGTTAACGCTACTTTAAAAACCTTAAACAAGATCAACACCTTGCAAAGCGAAGAGGGCATTATTCTGCCCCATCCACAAAAATATAAGAGTACTGATCCACAAGCTGTCGCTACCGTTCAGGGTCCAAGTATTATTGGTGTTCATGCTAACGCTAAGGAAAACGCTGAAACCCAAAAATTTATCAACTGGTTTATTAACCAAAAAGAAACTTGACCTGAAAATAGTAAGGGAAATAAGAATGGCCAAAATTGTCAAATGACCGCAGCACAATACTTTGCTAAGAGCTCTGGTTATGTTTTACCTTACAGTGAAACGTTTACTAAACAAAGTGAAGACGAACACAGCAAAACTAAAGACGCTTATAAGATCTTGAAAGATGTAAACGATGGTAAGTTAGTTGGTTACAGTGATCCAAGTGATTTCCGTTCTGGTAAATTCCGTGACACAATTGTCGCTGCTTTTAGTGGTGCTGTAAGCTCTAAAGCTGACTTTAATAAGTTCTTTAAAGATTTTGAACAACAATTAGGTCAAGAATACAGAAGGGGTTAATCCCACTTCTCCCCCACCCTTTCCCGGTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTTGACCAGGTGTTGGATTACGTGCCCTGGATTGGGAATGGGTACAGGTATGGGAAGAACCACCGGGGCGTGGATGATATAACCGCGCCTAAAACCGGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTCTCGAATGTCGGCGTTGGCCTCAAAGCCAACGTCCAAGCCACGCTTGGTGGCAGTCAGACGACGACTACAGGCGGCTCCAAATGACCCACCCTCGACCAAGCCAACCTCCTGCTCTGAACCGGCGCGGGGTGAAGGAATGATAAGAACGGACAAAGTGACGAAAACCACACCACCTTTAAAAGCGCTACGGGCAGTGGGCAGCAGGGTGGTTCTTCAGGTACCTCCGCGGGCAACCCCGACTCGTTGAAGCAGGATAAGATTAGTAAGAGTGGGGATAGTTTAAGTGTTGTGGATACGGGTCAAGGCGACGACACCCATTATGCTAATTTGCCACCAAGTATCACCCCCACATCCGACTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGGGTGCAGCTGTTCCTCCGCGGCCTGTTGGACAGCATCCCGGTGTTGGTGAATAAGAGTGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGCACCAAAAATGAAAGTACTCCGACTTAAAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGCTGTTGAATCCGGCCTTAGTTGAAACCTATTTTGGCACGACACGAGCGGGTGGTTCGGGGAACCAAAACAGTACAACCGTCCCCGGTATCGGTTTTAAAATTCCCGAACAAAATAATGATTCAAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCCCAGGACGTTGGTAACCTCGTTGTCAGTGGCACCACGGTGAGCTTCCAGCTCGGCGGGTGGCTGGTCACCTTCACCGACTTTGTCAAACCCCGGTCGGGGTACCTCGGCTTGCAGTTAAAGGGCTTGGATGTGAGTGAAGCGACGCAACGGGAGTTAATTTGGGCCCCCCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGCTGGGCCGGGTCGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAACTCACAGCGCGGTGTCCGAGTCGCAAGCAGCTACAAGTGGTACCACCACCGCAACAGGGGCTGCCTTACCGGAGCACCCGAATGCTTTGGCGTTTCAGGTGAGTGTGGTGGAAGCGAGTGCTTACAAGCCAAACACGAGCTCCGGCCAAACCCAATCCACTAACAGTTCCCCCTACCTGCATTTGATTAAACCTAAGAAAGTTACCCAATCCGACAAGTTAGACGACGATCTTAAAAACCTGTTGGACCCCAACGAGGTTCGCGCCAGAATGCTCCAATCATTTGGTACAGAAAATTCCACCCAAGCCCAGCCCCAGTCGCTCAAAACAACGACACCGGTGTTTGGGGCCATGAGTGGTAACCTCGGCAGTGTGCTTAGTGGTGGGGGTACCACGCAGGGCTCAAGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGGTCACTAACTATTAATAGGAACTTTTCTTATTAATAAGAACTTTTTTGAAAAAAGTTTTGAATTTATTTTGGTGTCTTTACTAACTTAAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGTTTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTATTGGCGCTTTTCAATAAACCGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGTGTTACTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAGGAATTTAACGAGATGATGAAAAAGACCAAAAAGGGCACTTTTTCGGGATGATACATCATTAATCCAGAAAACAACAGTGTGGAATTCTCTGGCAGTTTCAATAGACAATTTAAACTTAACAAACCGATTATCCCAGTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAGACTCAAACGACCAACGACTTATAAAGATTGAAACCACTTTGACCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAACAAATTAGAGAACTTCAAGTGGAGCAAAAAGCTCAGGGTGAGCAAATTAAAGCTCAGGGAGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAAATGAGTGATCGTTTGGACAAAATTGATCCACCCAAATAGTTTTAGGCTGAAAATAAAAATACTAGCAATTAGTCTTAAAAACAAGCTGTTGGTATTTTTTTATAGATAAAAAGTTAACTGTTTAAAAAAGCAAATGATTAAATTTTATTCAAATACAAATAAGTAAGTAATCACGATGAATTACTTAATCAAATGCTGTAAGTAGGTGAATAAATTAATTTGTTTTTCGTTAGCACTGTAAGTAATATAGTCTTTTAAAAAAATTTTATCCAGACTTTTTCTGCTAAGTTCATCTTGTCCTTTTAATGATCAAATCAGTGAAAGATCAAGCTGAATCCATTCTGTAATTATTTGTTGACTTTTATCTTGATCTTGAAAATTTTCATCTTTAACAATGAAACTTTTTTCTGTAAATTGTTTTTTTGAATGAGATTTAATACTAAGGAAAAGATCGAAATTATGCTTACCGCCATTATTGGCAGTGAAACTTTTTTCTCCAAAATAACTCCCATACCAATTAAAGATATAGCTTAACCTAGCATTAACTGAAAAGTCACTAAAGCTTTTATATTCCATATTGAAATACTCAACTTTCCGCTCCATTTTAAAATCGCTATTTTTTGTGGGGTTAGTTGCTTGATCGAAAGCAAGTTCCGCAATACCTAATTTACCCTCTTCAAAACTTTGTTTTCATGCGTCTAACAACAATTTTGTTGTATCTTTCGGATTAACTTCGTAAGACTTCTTTGAAGAAGTTATTATGTTCTTTTTACCATCTTTAGTTTTAATTGATTTAGACAAATCAATGTCTTTACCGGTGGCTTCTTTAATAAGATTTCTAAGATCAGCTTGGATACCAGTGCAAGCGCTTAAAATTGTTCCACATAACAAAAATAAAGAACCAAAAGCAAACCTTGACAAAAATTTCTTTTTGCGCATCAACAGTAAAAATAGTTCATCAATTCTGCTTTTTTTGGAAAAGCACGCTCAAAAAAGAACTTCATTGAAATTTTCGGTTGATTTTTTTCTTAAAAAAGAACTTTTTTAACTTAGAAAATTTACTTTTGACATTTTTATTCACATAAATAAAAAAAGCCATCTGCCCAAGGTGAAAAAAACAGACGGCTTTTTGACGATTTTGAAACTAACAGCAAGTTGTAACTGTTAGTGATTTGAATGAAAAAAATATATGACCTTTAGTCTTTTTAAAACAAATAAATCAAATGTGCTGAAAGCCATTACGTTAACATAAGTAAATTAAGACATAAAAAATGAACACATTAATCAAATAAGTGTGGGAACAGAAAATCAGGCTTTCAACACAAAATTTTCGTTTTTCTGCTCCCACTAAATAAATTGAGTTGGTTTTTAAAAACCGCCAAAAAGATTAAAGTTTTAACCCCACCGATCCTTATCGGTTTGCCGTTTTACTCGTGCCTCGCAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTGAGCTTACCACAGCAGCGAACCGAGAATGGTCAAAATACTTCAACCACCGGGGCAATGTTTGGCTTGAAGGTGAAGGATGCCCAAAAAGACGACCAGAAGTCCTCGGAATATTTAAGTGGGGAGGCGGGAAGTCAAGCGGGTGGATCCACCCAAGGTACTTCCACCACCACCCAACGTAGGGGTTCGTCAAATGAAAACAAAGTCAAGGTGTTGCAGGTGGCGATGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACCACCTCCAGTTCGGAATCTGGTCAATCAGGTACTCAAGGATCCACCCCAAGTAATCCGGGACCCTGAACCCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTGATCCTGTACGATGCGCCTTATGCGCGCAACCGTACCGCCATTGATCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAAATTAATAAATAGGTCTATAGGTTATATTAGTACACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGGGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACACCACCTTAAAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGAAACCAACAAGCCTCCCAAAAAAACCTCACCAACAACCCCGCCCCCAAGGCCGTCACCGGCTTTAAGCTCGACAATGGCCGCGCGTACCGGAAGTTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACGAAGGATGGCAAGGGGAAGGATAAAGATGGGTGAACCACTTCCGGGGCGAGTGAGGCCAAAGGAGATGCCCCGTTGGTGAGCTCAACCGGGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTTTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGTGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGGTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCTACAAATGAAGATTTGCCGAATGTAATAACACAGCTCTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGTGCGACTTGATGAGCGAAGACACATTTGAACTTCGGCACTGAAGTTCAAAAAAGCTTCGTTGAAAACCAGTTGGGGTTTAAAGATGACTCCAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGACCTCACCCAACCCGCCTACCTCATCACTGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAGACCAAGTATCCCGTTAAGGATGATAGTAAGGCTTCCGCCAAAATTGCGTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGGGTGACCGTGTTTGATGCGTTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGATCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACCTCCGATGATAATTCAAACACCAAAGTCAAGTGAACCAAACCGCCTCGCACTACCTCCCCGTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGAAGAAGAGCGGAGCAGCGGGAATAATTAGCATTTAAAAAGTTCTTTTAAAACAAATTTGAAATAAAAAGGATTCAAGAAATAAATTTCTTGTTTATTGTGAAAAATTCAAAAAAGCAACAATTTTTAAAGTGATTTCGCTTTGATTACAGACCATTTTTCAAAGCAAAATTAAAAAAGCTTTTTATCGTTTTTTGCATATAGAAAATTCAGCATTTATTAAGCTCAAAAAATAAGAAAAGCAGCAATTTCATAATTGAATTAAGTTTAATTCGCTAGTAGTGTTAAAAAGAAAAAAACTGCTCCAGGGTTTCTTGAAATTTTTACCGCTTATCATTCCCGCAACCATTTTTGTTAGCTGTGCAAGACGTGAAAGTAATCACCTCATCTTCAACATTTCACTCGATCACGACGCTGATGCTTCTATTAGTAAGTTCTTTGAACTTTACTCTAATAATTTAAGTAAAAAACTAGATAAAAAGGTTACCGTTAGCTTCAACATTATTGATGACTCTTTTACCAAAATTAGCAACATTCAAACTGCTAAAGCTGACTTTGCTTTCGTTAATTCTCAAAGTATTAAAGATAACGGAATAGAAGGATTTAATCTTATTCTTCAAACTCAAACTGATGCATTTAAGGAAGACACCAATTTAGATTATTACAGTGACGGTCAGTTGAAATCTAAAGCAGAAAAAATGACCACTTTATTTTCAAAAACGCCATATAAAGATTGAGAAGATACCGCACAACAATGAACCGGTAGCCGCTATAACTTTTTGTATGAAACAAACAAACTAATCAATTTCTACAGAGGCATGATTTTAATCACAGGTAGTGAGGAAGAAATTAAAAAAATTAAAGAAGCTTGAGATCAAAAGAAATGAAGTGATTTCATGAATTATGGCATCGGTCATGGCTCAAGTGGTTCAGCTGGAAAGTTCCAACTACCTGATCTTTTATTAAGAAAGCACTTTGGCTCTAGTTACCCCGGTTTGCAAAATGCAATTAACCAAAACCCTGATAAGTTTGCTAATGTTAGAGGTCGAGAAATAGGTAGAGACAATAAAATCAAAATTGTCTTTGATGATGCTAATTCCTTTGCTTGAACTCACAACGACAAGAATGCAACTAATCACTTTTATACACCAACAGAAAATAATGGCAAAGGTGATTCAGAAAAAAGCAATAATAAAAATAATAAAGTCGAAATCTTAACCTATACTGATCCAATGCTGTATGACATTGGCATTGTAAGTGATACTTTAAGTGATCGCTATCAAAAAGCAATTGCTGAAGTTTTTGTGGAACTAGCTAAAACAAAACAAGATATTTATGGCCCTTCATATGGTTACAACGGTTACAATTTGATTACGGACCCTAACAAAGAAATCTTGGATGTAATTCATAAAACATATGGTTAATCCAGTCTTAGTTTTTGATCAAGTTTCCCTTAAATATAACGGAGCACCACTTTTAGAAAACATTAACTTTACAATTTCACCTGGTGAACACATTTGCTTGTTGGGTAAATCTGGTGTTGGTAAAACTAGTCTTTTAAACTGCATTACTAACACCAAAACAATTTCGAAGGGAACCATTTATTTCAATGGTATTGCTTCTAATAACAAGGATTACAAACAATTAAAAAAACAGTTCAGTTTTTTAGATCAAGTACCTAATTTAATCGACACTGACTTCGTTTATGACGCCATTTGAAGGGAAGCGAAAAATAACCTGAAATGGTGACAACGGTTATTTTTAGTGGAACCTCAATCGCTAAGAGAACAAATTATCCAAATCCTTGAAGAGGTTAACCTAAAGGAATATGTCACTTACATTATTAAGGACTTGTCAGGTGGTCAAAAACAACGTGTGGAAGTAGCTAAATTGTTCTTTGCCAATTCCCAAGTTTTATTAGTTGATGAGCCCACTACTGGACTTGATTTAATCAATGCGCACAAAATCATGGAACTAATTACCCAATTTGCGCGCCAAAAAGCAATGACCTTAATCTTTGTGACCCATGATGTTGAGTTTGCGCTCAAATACAGTGACCGTATTATTGCTCTAAAAAATAAGGCATTAGTTTTAGATCAAGCTACAAATAAGCTTACCAAACAAAAGTTAATGCAAATTTATCATGACTAGCTTGTTTTTCTATCAAATATCAGATAAGCAAAAGCGTTGAAATTGGTATTGAAAGTTAGCTTTAGCGATTATTGTTTTAGTTGTTATTATCTACAGCTTTATCGATAACTTTTCCGGTTTTAACGTATCTGGTTTTAGGAATTTTAGTCGTAATTTTATCCGGTTATTCACCCCTGACACAGCACGTGATTACTTTTTGGGCTCTTACCTACTGCAAACCATTTTTTATGTCGTGAGTGGTAGTATTTTGGGATTTGTCATAGCCCTGTGGTTTTCGTACCTCACTGCCTTTAAAATCCAGCCACTTTACATCGCTTTACCAACTCGCTTATTCACCATTTTTCTGCGTTCCTTTCCAGTACTTGTGTTTGCTTTTTTGTTTAACAATTTGTTTAACAAACAACTAACAGCAACCTTAACGATTACCTGGTTTAGTTGATTGTGAAGCACTAAGTACATAACTGCTTTTTTTGAAAACAGTGCTTTAAAACAATTTTTTAACCAAAGCTCTAGATATATACATAAATTCAAAGCTTTTTGGAATACCGTTGTTATTTCCCAAGCTGAGCGCTTATGGTTGTTTTTGCTCTACAGCTTGGAAGCTAACTTTCGCTGAACAACAGTTTTGAGTATTGCTGGCATTACTGGAATTGGTGAGCTCATTGCCACACCTCTAGGGGGCACAGTGCAGCTTAACTTAGTTTTAATACCGATGTTAACTTTGATCGGCTTTTTACTGTTTTTAGAAGCGAGTGTTTTTCTGTTAACTAAGTTTGTTTTACAAAAACAGAGCCAAGCCGGTGATTACTTTTTACAAGCTAAAACACTGCAAAAGAGAAAGTGAAAAAAAGTGATGATTTACATCCTAGCTTTGGTTTTAGCGGCTTTTACCTTGGCTAATTTAGTACAGTTGGACTATACGGTTAAAGCGCCAGGGTTTGTGGCTGACTTTTTTAAACAGTTCTTTCAAACAAAAACAGCTTTTTTAATTAGTGAAGACGCTAACATTAACCCACTTTTAATGTTGTTAAAACTCACAACTCAAGCGATTAGTTTAATTACCTTAGTGTTTGTGTTAGCACTCTTGTTTGGCTTTTTAGCATCAAAGCTGTTTTCCACTATAACCTCAATTAGCTTAAAACTGTTACTTTTAGTGATTAGGGTAATTCCCAGTGTCTTACTGTTCCGCTTATTTGATCCAATTATTTTTCGCCCAGAAACTACCATTATCTTTGTTTTAGCAATCCACAGTGCTGCTAGTTATGGTCAACTGATAACGATTAACTTTGATAATGCCAACGAAGGGGTAATTAACAACATGCAGAACCACGGCTTTAGTAGGTTTTACATCTTGTGAAATTACCTCATACCAACCACTAAGCCCCAACTGTTAAATACGTTAAGTGATAGCTTTGATAATGCTATCCGCGACTTAGTTGTGTTTGGGATCTTTGGTGGTTCGATTATTGGGGGGCGAATTAACAATTTCTTTGAGCGAGCACAGTACTCTGAATTGGGCACAATAACCTTACCCTTAATGGTTTACTTAATGGTGTTTGAAGTCATTTTAATGGCAGTACGGTTAAACAAACTCAAAGTGTGACAGCGCCATCTGTGGTAGTTAATTGGGTTTTTGTTGGTTTAAGAAACGCTCTAAGATTAAGGTAGCCGCTAAGGTGTCCTTGGCCTTTTTAAAGGTTCTGTTTTTTAAGCCTAATTCCTGCAAGTTAGCTTTTACCTCACTCGTAGTACCACTTTCATCAATGAGTGTTACTGGTACATTAAAGCGGGTTTTTATTAGTTCCACAAACTTGTGAATGCTCACTTGGATACTTGACTGGTAGTGGAAGTGGGGAAAGCCAATCACTATTTGCACTAATTCATAACCAGCTTGCTTTAAATCCTTAAAAAGGGTATTAACGGCTTGTTGCAAATTATTTTGTACCGCAAACACGCGGAAGGGACTTGGAAAGCGGTCAATGGTATTGGCTAGTGCTGTACCAATTCTCTTCAAACCAAAATCAATGCCTAAAATGTACTGCACTATTGAAGGGTATCGAGGATAGCTTGTAGTTTACTTACATCCACCGACTCGTTAAACGAACCCCTAAAGAAGTTATTACTACCCCCACCCTTAAGTTCAAAGTGAGCTTTTAAAAGCTGAATACATTTAGAAACATCTTGCCCTAATAACATAAAGGACGGACTGTTGTTGAATTGGTTGAGGAGTACAAACAGCTTGTTTTTGTGTTGGTTCAAGACTGCTTGGGCTACTTGTGATAACAGTTTCGGATCAACTGCTGCAAAGCTAGCGAGAACATAAGCTTTGTCTTGCTTTGCTAGCTCTAACAATTCCTGTTTGATCTTTTCCGCTAACTGCTTTTGCTTCCACTTAAAGTCTTCCGTTTTCAGTTCATTAACCGTTATTTTGAGTTCATTTAGTAAAGCTTGGCATTTACGTAAATCAGTAATTACTTGCGGCAAGTGAAAGTGATTTAGTTGCTTAGTTAAAGCAGTTTTTTGCTTGTTCAAAGCGGGGTTAGCAATGTTGTTTAGTGTATTGTGTAAACTGTTAATGGTTTCACTTAACTTGCCATTCTGCTCATTGAGGTAAGCTGCAATTGTTTCGTTACTACTAATGATTTCAATCCGTCAACGCCCTGTGCCACGAGCATAGTAATCAGTAATAAAACAGTTCTCAATTAAACCAGTGTGTTCCACATGGGTACCACCACAAAGCTCGACACTAAAATCACCAAAGCGAATTACCCGTAACAACTCGTGGTTCGAATACTCCTCTTCAAAGTAGGCAATCGCATTAAGTTTTTGACTACCCTCTAGATCAGTAAAGATCTCTTCGCGCTGAATGTCTTGTTGAATAATTTGTCGAATCCTGTGTTCCACCCGTTCCAGTTCGGCTCAGGTTAAGGCTCGCGAAAAATTAAAGTCAATGGTAGCTTTTTGCGCTGACTTAAAGGCGCCGTCTTGTTTAATCAAGGGGTCAATTTCCTTTTGCAATGCCGCGTGCAGTAAGTGTTCTAAACTGTGGTTGTTTTTCACTAGTTGGCGCCACTTACCGTCATGTACTAGGATCACTTTGTCACCTAGTTGAAAACTACCAGTTAAAAAGAAGTGGAAGTGTTGTTTATTAGGGCCTTTAAACACGCCCTGAAAGCTTACTCTTTGATCATTTTGGCTGTGGTTAATACAGTACCCCTCATCATAACGCTGACCGCCTGATGTGGCATACAGCACCGTTTGGTCAAACACCACATAACCACTTTGGTTGTGCAGTTTTTGCACCGGTTGGTACTGTTCATCAAACAGCCCAATGACCTTGGCGGTGATCTCATTAGCCTCGTATAAGAACTCACTCGCTGTTTTAAAGTTCACTAAGTGTTCATTTTGCTTTTCAAAGCTCAACGTATTACTGTTTTGTTTGGAGATCAAACGGTGCTGTTCCATCAGTTGTTCCACGGCCTTTCAGTCTACCTGGAGTTGGCGCTTATCCACCAGTTCCTTAATCATTTCAATCGGAAAGCCATAGGTTTCGTACAACTGGAAGGCTACTTGGGGTTTGAGTGCATTATGAGCAATACTCTTTTCAAACAGCATTAAGCCCAAGTTAATTGTTTTATCAAAGGCCGTAACTTCCTTTCAGATTGCTTGTTGCACCACCTGGTTTTTCCCCTGCAACTGCTGGTAGTAAGACCCATAAACGCTAATAATGGCATCGACCATCGGATCAATAAACTTTAAGTCTAAGTCCAGTTTTTTGCAGGCCAACAAAGCGCGGCGCAGTAACCGGCGCACAATGTAACTGCGCTCGTTGGGTCCTGGCAATACGCCTTCGGCAATGGTGAAGGTAATTGCCTTAAAGTGGTCACTAATAATGCGAAAGGCACTTTGCGCTTCGTTTAACTTTTGGTCAAACTTAAAGTATAAGTTGGTATCATACTTATGCTTACAGTGCTGTTCAATAATGCCAATTAACTTTAAGAAGATATCCGTGTCAAAGTTAGTCGGCGCGTCCTGTAAGATTGCTACTAGGCGTTCAATACCGGCCCCAGTATCGATGTTCTTCTGCGCTAACTCACTGTAGTTACCATTGCCATCATTGTTAAACTGACTAAAGACAATGTTCCACACTTCCACATAACGGTCGTTTTCAATGTCCTCAAAGAACAGTTTTTCACCAACCTTGTGCGGATCAAACCGCTCCCCGCGGTCATAGTAAATTTCCGTACACGGTCCACATGGTCCTAAACCTAAGTCCCAGAAGTTGCGGTCGCGGTCACACTTAATAATCCGACTTGCAGGAATACCGTGTTTAAGTCAGGTGTTAAAGGTCAATTCATCATCTTGGTACACTGTAATGTAAAAGCGCTTCGGATCCAAGTGGTAATGATTGACTAAAAGATCATTGGCAAAGCCAATGGCCTCTTCCTTAAAGTAATCATCAATCGAGAAGTTACCCAACATCTCAAACAGCGTTTGGTGTCGCGATGTAAAACCGACGTTTTCAATGTCATTAACCCGTAGACAAATCTGGGCGTTGGCTAACCGTTTACTCGGTGGTGTTTTCTTAGCACTAAAGTAGTCCTTTAAGGTCGCTACCCCGGAGTTAATCCACAGCAGTGAAGGATCATTTACGGGAATTAACGATTTGGAAGGGAGTGCTAAGTGGCCCTTGGCAGTAAAGTAATCTAATCAGGTTTGTCTAACCTTATCAGTTGTTCATTTCATTGGTTTAATTGTAAATGTTTGCCTTAAACCAAGGATGACCACGCAACAAGCTTACACTTCAAACTTCTTGTTGGCAATTTGTGCATACACCATTCGATCTTGACGGAACTGGTGGAATTTAACCTCACTGTTAAGCGTTCACAGCCCTAAAGGTAACTGCAAGCTCAACACCATGTCCGGGAACTTTTCGTAAATGTTTGTTCAGGGATGGAGATACTGACACACTTGCTTAATTTTGAGTGCATCAACTGCTTGGAACTGCTTTTTCGTTCAAAACAAAAAGGCTATTTGGTAGTTGGGGTTAATGTCATACAAGCGCTGTAAGGAAGCAAAGTTAAACGAGGAGAACACTACCTTTTCAATTACTTTTTTGCCATACTGTTGGGCCAAAGCATCAATTTTAGCTTCAATTCCCGGGTATTCCTTTTGGTCAGTTTTAATTTCCACGTTAATTAGTTGAAATTTATCTAAGTACTGATCAAAGAACTCCTTTAAGGTCATAATCGGCTGGGGTTGCGTTTTGAACTTAAAGAAAGCACTGTGATCATCTTGCTTTAAACTAGCGAGTGTTTCCAGTTCGATTGTTTTGTCGACTAGCGCTGTTCTGGTGGTAGTTTCATCATGGATAATCACTAACTCCCCGTCCTTGGTCAAGTGCACATCTAACTCCACGCCATCAAAGCGATATTGGAACGCTAATTCAAACGCTAGCTGGGTGTTTTCAGGCGCAATATCACTGTACCCACGGTGTGCTAGCAGAAGTTGTCGTTTAAGCATTGTCTAAGGTTCTCCAGCGGTAATCGGTTTTACCCAAGCGCTTTAACTCTCAAGTATTAACCAAAAAGACAATCGTACCACACAATAAACCAAACAGGGCTATTCCTAGACAAATAGCTGCAATAATTTGGTAACCCTGAATCGAGGTATTCTTCTGACCGTCTGGGGTATAAGCCTTACCCACGACACTTGTAACCGTGTAGAGCCAACCATCAGTCGAAAAACCAATAAAGGAAAGTAAACCAACACTAGAAGCATAGTTGTTTTTACCAATCTCTATTTCCGCTACCTGGTTGTACCTAACGGTTACCATACCCCAAGACAACACCCCGGTGAGAATGTAGAGGATCGCCGATACGATAATCAAGGCATAGTTTTGCTGTTGGCCCACTTGCCCAAAACCTAAGAGAATAAACATCAAGACAAAGATAATGCCAAGTCCTGTACACAACATTAAAAAGAGGATGTAGCTCCTAAACTTATCAGCTAACCTGCCCAAGTACACACTTACAGCACTGCGCAAAACATACGTCCTTACACCACCCAGTACAGTCCCTAAGATCACTGGTGCCAAAAAGGCGTTTTGCATCATTTGTAAAAGATAATAAGCAAACGTACTTTGAAAGACATACATTCCCATTAAAAAGAAGGACAAGAGTCACAACTTCCAGTTCTTTAAGGTAATGAGAATCTGTTCAAAGTTCTTTTTGGCAGTTTTTAAACTGGTATGACTGTCGTACTTTTCAATTGGTTTTTCTGGTACAAAGAACAGCACGGTAAACCCAGTAACGAGCAACATGCCACCAATAATAAAGGCATAAATGGCAAACGGTGCGGGATTATTAGCACTACTTTCACCACCGGCTGGGTAAGCTACTGCTGTAATAATCAAGGCAATTAAGAAAATAAAAATAAAACCCCAAACACCATTAGCAGCGCCCTGAATACCAAAACCCAATGCTTGGTTTTCCTGGGTTGCTTGTTGACTCGCTAACTTTCACAGTGGTGTTCAAAAGATTAAGGTCGAAGTAATACCCCAAAGCCCCCAAATAACACAGTACTGTACAAACAAGGCATTGGATTTTTGGCTTTCACGAGTAAGAATGTTAGTAGCTAGCCAAAAGGTAATTACACCCGTTGAAATTTCTGACAAGAACAATAGTTTCCGCGAACTAAACCGGTTCGCTAAAAAACCACCAGGTAACTGCGTGGCTAAGGTCACATAACCAATAATCGAGGTTAAGGTGGCCACTTCATCTTCAGTAATCCCTAAATACAGGTGCAAGTTAGGCACCACATTCTTAATATAGTACGGGGCCGCAATTACAAAAACATCAGCCGCACCGAGAATAATTAGTGCTATGAGCTGCTTTTTGGAAAACTGCTGCAACCGTTTCTTTAAACTAAGTTTCGTTTCCATTGTGACACAACGAAATTACGCCATTATTTTAGTGGTAATTATGGGGTTAATCCGGTTAGCTTGCCAAAAAATAAATTAGAAAAACGTTTTTGGCATACATTTATTGCAAAAAGTGAGAGGAAAACCGTATTTAGATTTGGCTAGCGGTAATGATACCACTCCCCAAACAAATGCCATTCTGGGTATACAAAACACCATACTGACCGTTGGTAACGCGGTCAATTGGTTGATCTAGTTGTACTTGCACGACGTTGTTTGGTTGTACTGTTATGTGCCCTTGCACTAACGGTTGGGCATGGCGAATCCGTACCAGAACGGTTTGGGTCAATGGATTGGGTTGGTACAACCAGTTAAAGTGGGAAAGGGTAATGCCACGACGCTGTAAATCTTCTGGGTCACTCGAGACATACAAGGTGTTTGTTTTTAAGTCCTTTTCAACGACAAAGTTCCGTGCTGCTTGCCCACCTAAATTTAAGCCACTGCGCTGCCCAGTGGTGTAAAATCACACCCCTTCATGACTGCCTAGTTGGCGCTGCGTTTTTCAATCATAAATAACCCCCGGTTTGACTGGTAAATAATTCTTTAAGAAGTCACTAAAGTGGCGTTCCCCAATAAAGCAAATGCCGGTTGAGTCCTTTTTAGTAGCCACACTAAAGTGGTGTGCTAACGCTATTTGCCGCACCTCCGTTTTCAGTAAATGCGCCAAGGGAAACACCACGTCTTGTAGTTGCGCTGGTTTGACATGCGCTAAAAAATAGGTTTGGTCCTTGTGCTTGTCTTTGGGAATGGCCAGTAAAGGTTGGTTGTTGTCCCAGCTAAGTGCCGCATAATGCCCTGTAGCAAAGTCACTGTTGGGATCTAACTGCTTGCAGTAATCACGCATTAGCCCAAATTTAATTAAACGGTTACACAACATGTCGGGGTTAGGGGTTAACCCGTTTTTAAAGGCATCAATGGTAGGGAGAAATACCTGCTTTCAGTACGGTTCCACCAGGTTTTGCTTAATTAGTTTGATTCCTAGTAATTGGGCAATTGCTTGCGCTTCGCGAAAATCTTCTTTGGACGAACAACCCGACTTATGCTCGTTAAAGGCGCGGTGACCTAACTGGTCGTTATTTAGTGTATTGTCCCAGCACTCCATAAAAATGCCAATCACTTCGCGGTACTGTTGCTTTAAAAGGAGCGCACTCACAGCTGAGTCCACTCCACCTGATATTCCGACAAAAACGGTTTTATTTTGCATCTAGTAAACTGTTAAAACACCAAAGCGCTTACCCAACTGCTGGTGGTAACGCCCGACATCAACACCCTTAGTTATGGCCAGCGCTGAAATAAATAAGGTTAACGCTTCATAAAAGCTCTGACAACAACTTTTGACATCACCTTTAGGATTGTCCACTAAAACAGCTAACAAATCCAGTTGTTTGACCACTTCACTACTAATAAAAAAGGCGTCACTGAGCATCGGTTTTAAAAAGAAGGACTGATCATCATAATCATCATCTTCATAAATTAACAGGGTACTCTGTTCGCGTTCCAAACAACGATTAAAGTTTTGGCGAAAGGTCTCAAAATCAACTTTTTCAAACTCAGTTTGCACAAACTGCTTCAAGGCTAAAAGTTGTGGTTTCATTACCTTAGTTTAATAAGTTGCTCGCGTAATTGGGGATCTGTTATTTTTTCGTACAACTTTAAACGGAGCTGTTGCTTTTTAAACAACTGCAATTTCGCTTCAAAACGGTTAAAGATCAGGTTGCAGGTTTTGATGTGTTGGTGGATTGAACTTTTCTTAACTTGGTATTTATCAGCAATCTCTTGCATGCTCAAATCCAGGTTAATGTACTCGTTAAAGTAACACGCTTGCCGTTTGGTTAAAAGTTCCCCGTAAATATCAAATAGTTGCTGTTGCTTTAAACGCTGGTTAAGTTTGGTTTGGTTCATGGTTGAGGCTTAAATTCTTGGTAAGACCCAGTACATATTGCTCTAAATCAAAGGCCGCTAAATCAGCAGTTGTTTCTCCAAAACCAATCAGTTTTACCGGTAAATTAAACAGGTCTTTAATCGCTAGGATAATCCCACCCTTAGAGGAGCTGTCCATTTTGGTAAGGATAATCCCGGTAAGCTCGGTAAATTCATTAAAGACCTTGGCCTGTGCTAACCCCGATTGTCCGGTAGTACCATCTAAAACGAGCAGTGTTTCTTGGGGTTTAGCGCTACTCACCTTCTGGACAATTTGGTAAATCTTTTTCAACTCATTCATTAAGTTGGTTTTGTTTTGCAACCGTCCCGAGGTGTCACACAACACAAAGTCGTATTCGTTTTGAATGCCTTGTTGCACCCCACGGAAGATCACCGCTGGCGTTTCCTCTTTGGGATTGGGTAAAACAATGTCACAACCTATGCGCTGCGCTCATTCTGCTAACTGGGCCACTGCCCCAGCGCGAAAGGTATCCCCAGCGACCATTAACACCCGTTTGTTTTGCTTGGTAAGCTGGTCAGCTAGTTTTGCTAATGAAGTTGTTTTGCCAACACCATTAACCCCGACAAACAAATACACGTTGGTTTTATTAGCTTCAACCGTTAAATCAGTTTCAAACAACTTATCTTGGATATAGTAAACAATTAACTTATCGACAATAATTTCCTGGATTAACTGAGGATCACTCACTCGTTGTAGTTTGAGTTCTTGGACAATCGCATCACAGATTTTAGTAGCCGCATGGTAACCAATATCAAGCAACACTAACTCCTCAAACAAACTTTCTTGAAACTGTTCGTTAACCGTTACATAGTTAGCTGCTAGTTTATTAATGGTATTGGCAAAGCTGTTAGCCGACTTGTTTAAACCCTGGTAATAGCTCTTTTGGTTGGCTTGAAAGAAGCTCTTTTCCTGGACAGCTTGTTGCACTTGGTCGACCAGTTTCTTTTTCGGTTTAAACTTCTGGATTAGCTTGTGGATAAAACTCATAACAATTAGTTCTGCTTATCTTTACTAATGTACTGCTCTGCTTTTTCCAAAGAAACGGCAAACGTCTTGGTCACTCCCTTGGTCTGCATTGCCGCTCCCAGTAACATGTCACACTTCATCATCGTGCCCTGACGGTGGGTAATAATTAAGAACTGGGTGTTGTTACTGGAATTGCCAATAATGTTAGCAAAGCGCTCCACGTTCGCCGGATCTAAGGCACTCTCCGCTTCATCTAAAATTACTAGAGGAAAGGCACTAACTCTCAAAATACTAAAGAGGACCGACAAGGCCACGAGTGTTTTCTCCCCACCCGATAACAACATTAAGTTAGCGACATTTTTGCCAGGCGGATTAGCAAACACTTCTATGCCTGACAACAAGACGTTATCAGTATCCGTGTAGCGAATTTGACACGAACCACCGCCAAAGAGGTAGTTAAAGGTTTTAGGTAGTTCTGCATTAACTTTTTGGATGAGCTCGTCAAATTCCTTGCAGGCCAATTCATCAATTTCACCAATCGCGGTTTGCAAATCCTTAATCGCCGTTTGCAGTGATTCATACTCACCGTTAATGTCATCAAAGCGCTTTTGCTTATCAGCAATTTCAGCAATCGACTCCAGGTTAATTGGACCCATTTCATCGAGTTGGCTCTGCATCCTGGCAATCTTGTTTTGCGCTTGCATGGTGGAGAGCTTAATTGGTTTGTTATAGTTAGCGATCGCAAACTCCATCGTTAGTTTATACCCACCGTTAATCTTGTCGGTAATGTTGTGGATTGTGTTTTCAAAGCGGATTTTGCCTTCGCGTGCCAACACTAACTGGGAGCGTTGTTCATCTAAAAGGGCACGGAGGTCAGCGGTTTTGGCTTCGGCTAGTTTAATCGATTTGGCCAGTGTTTCTTTCAATTCCTGGTTGAGCTTTAACTGTGAGTTGAGTTCGTCACGTTCAGCCCAAGCACGGTTTAATGATTCAACCAATCTGGTTTCATCAAAGCTGTGCGGGGTTCCGTCAAAGCTTTCGTTGGTGAGCTGCTCATACTCAATCTTATAGCTCAAAATTGCCTTATTGGTGTGGTTAACACGTTCGTTAAACTTTTCCAGTAGGATTTCTTCGTACTTTAACTTTTTGTTGAGCTCGTTGAGTTTAGTCTCTAAGCTAGTGAGATTGGTACGTAGTTGCTTTTCGTTATGTTCTAGTTGGGTAATGTTAGCTTCACTAGAAGCTAACTTTTCCTGGAGGTTATACAAGCTTAAGTTGTCCTTGACATAACCACCCTGGATAATCCCTCCAGCGTACACCGTTTCCCCGTTGAGGGTGACAACGCGGTACAGCTTATAGGTATAACTGGACAGTTTTAAAGCGGCTTGAAGGTCACTGGCAATAATTACTTGACCCAATAAAGCATTAACAATTGGTTGTAACCTTTCATCACAACTCACATGGTCACTACCAACACCCAAAAAGCCATCGAGTTGTTGCAAAATTTCCATGTGCTCGGGAACTATCTTAGTATCAAAGGCAATGTCGTCAATTGGTAAAAAGGTAACTTGACCAATCTGGTTAGTTAACAAAAAATCAATTGCCTTTAAAGCCGCCAAGTTGTTGTTAACAACCAGATAACCAATTGATTTACCCAACGCCTTTAACAAGGCCTTTTCGTACTGCTTTTCAAACTTTAAAAAAGTGTTGAGCGTGTTTAAGAGACCGGTGAGGGCCTTGACATTTTTGACCAAAACATGGGCCGGATTCGTTTTGACCCACTCCTCGTTTTGCGCTTTTAAAAACTCTTGCTGCGCTTGTTGGTAGACTAACGCCGCACTGTTTTGGTTAAGATTCAACTTTGTGGTGTCCAACTCAGTTTGGACGGTGCTCAACTCATTGGTTAACAGGGTAATTTCACTGGTTGTTTTGAGTACTTGCGCTTGTAAGCTTTCTAGTTGCGCTTGGTCTACCCGGATTAACTTTTTTAACGCCTGGATTTTGTGCTTTTCGTCCTTTTTGGACAACTCGTTTTTGAGCTGCACATCAATCACGGCCTTGCGTTGTTCCAACTCGTTAATCGTTTGGTAGACCGCTTGCAGTTCTTGTTGTAGTTTGATGGACTGTTCATCAGCATCACGAAAGCGCTGGTTAAAGATGTTGAGCTGGTCTTCTAGCAGTTGTAACTGCGGTTCGTGTAGTTTAAAGTCCTGTTCGATGTAACCAATTTGGTGGTTAAAGCGGTCTAACTCACCCTGGGACTTGAGGTAATCTGCGACCAAGACACTCAGCTCCAGCTCCTTTAATTCTTCCTTAAGTTTGACAAAGCACTGGGCCTTGTCCGCTTGAATGGTAAGCTTCTTTAAGTCCTTTTTGAGTTCATTTAAAACAATGCTGACCTGTTTGAGATTTTGCACGGTACGCGCTAATTGGTTGGTAACCTCTTCTTTGCGCTTAGTGTACCGCCCAATCCCCGAAGCGTCCTCAAAGATCTTACGTCGCTCCTCTGGTTTGGCTTCCACAAATCACGACACACTGCCCTGGGAAATAATCCCCAACGAGCCTTTTTCCAAACCAATATCAGCAAAGATCCCACTAATTTCTTTTAGCGTTACTAACTCGCCGTTAATATAGTACTCACTCTGACCACTACCACGATAGATCCGGCGCATCACCGAGATCTCTGGTCGGGGATCGTGCAGTAGTTTTTGGGAATTGTCAAAGGTCAGTTCCACTTCCGCTAGCTTACTAGCGGGCTTGTCCTTAGAACCAAAGAAGATCATATCATCACCGGACTTACTGCGCAAGTGCTTCATGCTGCGTTCCCCTAAGACCCACTTCAAAGCATCGACAACGTTGGACTTGCCAGAACCATTAGGACCGACTATACCGGTCATTGAGTGGGTAAAATTAATTGTTATTTCGTCAGCATATGACTTAAAACCATACGCACGAAACCGTTTGAGAAAAACCATTTAAATCAGGTTAGATTTTGCAATAGTGCATTACTAGATTATACTAGTTGGCTTTAAGGAAAGGTTTAGCGCCCCTCAACTAGTTGCACCAACCACTTGTCTTTGGCAAACTAGTACTTGTAATACTTACTTACAATCTAACTATCATGAGCGGTTCAGTTAAAAAAATTAAGCTTAATTAAGTATGACAAAGACAAGTAAAGCATCCGGGTTAAGCTGGTTTTTTTGTGATTTAGATGGCACTTTACTGCGTTACCAAAACAACCAGCACTTAATTGAACCAACTACGAAGCGAGCAGTGGCACAGTTAGTGGAGAGTGGTGCCAACTTTGTTGTAGCTACTGGACGCAAACCGAGTGACGTGCGCAACATTTACAAGGAGTTGGGGATTGAACAAGCCAGTCCCTACTTAATTGCCAACAACGGCGCGGTAGTGTGGGATTTAAAGCGCAATAGCTACCTCAACAAGCAAACCTTGTCTTTAAGTGACTTTGACTTAATTGACCACATTAACCAAACGTTAAACCAATTAAACCACGAGTATGGCTGCATTCTGTATGGCCTCAACGACCAGGTGTACTTTTATCATATCCACGCTCCTGACTCACAGGCCTTCAAACAGTACTTTGCTTTTTATGAAGGCGAATTTGTCCAAAACCAGTATCTGGAAATAGATGGGCTCAAAACTGAATACAATCTAGTTAAAGCCATTTGGTTTTTTAAGGAAGTGCACCAGCAAAAGGCGGTGATTGCGCAACACTTTACTAACCAAGAACGCTTGGTCATTACTTCAGCACACTCGTTTGAACTAGTACCGCTTAATGTATCAAAGGGTCATGCGATAAACCTAATTAAACAACAAGTAAAAATCACCGATAACCAGATTATGGTCCTCGGTGACTCTTACAATGACTTGCCAATGTTTCAACATGGTGTAGTTAAGGTAACTAATCACCTCGCACCCGACAACCTCAAACAACTAGCTACAAGGGTTTATGACCTACCAGCTTCACTGTTTGTGGGTCAAGCGTTGAACGACTACTTTAAGTTCGATTAACTTATTTATTTAAGTGTTTGCGCTGCGGTAATAATGCCCACGTTAAAGACATCACTGACACTGGCACCACGGGAAAGGTCGTTAAAAGGACGTGCTAGACCTAAAACAATTGGACCAATTGCTTCATAACCACCTAAACGTTGCGCAATCTTGTATCCAATGTTACCAGCATCTAGGTTCGGGAACACATAGATGTTAGCTGATCCATTCCAAGTCAAATTAGGGGCTTTTTGTTTGCGTACCTTTTCCACAAAAGCACTGTCAAACTGTAATTCACCACAAACATTAGCTTTGAGTTCGGGGTGTTTTTCCAAGAAGATTTGGGTCGCACTGACTACTTTGTCCACCGCTTCCCCTTTACCACTGCCCAGTGTTGAATAGCTCAAAAAGACCATCTTGAGTTCTGGTTCCCCCATACTTTGGGCAAACTTAAAGGTGTTTTCCGCTACAACTGCTAATTCTTGGGGTGAAGGGTAAACAGCAAAAGCACAGTCGGTAAAGTACAAGCGTTCCTCATTCTTTTCCATGATGAACACACTCGACACAAAGTTACCAGTACCTAACAGTTGTAAAGCAGGACGGAGAGTATCCTTCGTAGCGTATTCCTTACCACACACTTCCCCATCCACTTCGTTGAGTGCTACTAAAGTGGCTGCTAGGGAACTGGCATCACGGACAAACTTTTGTGCTTCACGCATTTCCATGCCCTTGTGCTTGCGCTTTTCATAAACGAAGTTAGCGTACTTTGTCAAGTCCATTTCCTCAATCACATAGTGCTTGATGGCGGTGTTAAAACCAGCTGGTACTTCACTGCGGGTGCGGAAGATCACCGCTGGGGTTAAAATACCCGCTTGCTGGAGCTGGTTAACTGCTTCCATTACGGTTGGCGATCAACCTTCAGGGAAAATGATCACTGGCTTTTTACCAGCACTTTGAACACGTTGTTTTAAAAGATCAATAACACTCATAAAAACCTGCTTACTTAGTTTTACTCTTTACTTTAATTTACTTTTTTTGAATATCGCTAATTCCCACTAGCTCTTCCCCACCTATTAGTGCTAAAGATGCACCACCACCAGTGGAAATAAAACTAAATTGATCAGCAATCTGGAGTTGTTTAGCAGCGGCTGCAGAATCACCACCGCCAATGACACTAAAGGCTTGTTGGTTTTGCGCAATCGCTTTACCAATTGCTAAGGTACCCTGAGCATAGTTGCTAAATTCAAATACGCCCATGGGACCATTCCAAAAGATTGTTTTGGCCTTGGCGAGGTAACTGCTAAACAGGGCCACTGTTTTGCTACCCACATCCAAGGACATAAAGCCCTGAAGTTCTGCTGGTCATTGGGGGGCTACAACACACTCCACGCCTGGTTCATCCTTAAAGCTAGCATGAAGCATTTGGTCTACCCCCAAGACAATTTTCTTGTCCTTGTCACTGTCAAGGATTTGCTTGGCGGTTTGGACTAAGTCTTCCTCCACTAAGGAAGTGCCAACTTCGTTACCCAGAGCTTTGAGGAAGGTGTTGACCATGCCCCCACCTATTAAAATATGATCCGCAATCTTGAGCAGGTTATTTACTACCTGTAACTTATCAGAAACCTTAGCGCCACCCAACACAACCACAAACGGTTTGGGTGGGTTATTAATTAAGCGGTAGAGGTTGATCAATTCCCGCTCCATTAAAAGACCAATGCAGGAGTTTTTGATGTACTTGGCAATGCCAGCGTTGGAAGCGTGACGGCGGTGGGCGGTGCCAAAGGCGTCATTGACAAAGATGTCACCCAATGATGCTCAGAACTGTGCTAACTCAGCGTTATTCTTGCTTTCGTGTTTAACCACTTCACCAGCCTCATTCACATCACAGTAACGCGTGTTTTCCAACACCAAAATTTCCCCTAATGGGAGTTGATTGACTGCGACTTTGACCTTGTCACCCGTGTTTTCCGGACAAAAGTGAACCTTGGTGTCCTTTAATAAATTTTGTAAAGCACTAGCTACTGGTTGGAGTGACTTCTTACCCTTTTTGTCATCGAGCGATTTGACTCGTGACAAATGGGACAGTAACACAATTTTGCAATTGTGACCCAAGAGGTACTTAATCGTGTCTAAGCTAGCTAGTATGCGTTCGTTGTCGGTAATGACCCCGTCCTTCATCGGCACATTGAGGTCAGCACGTAAGACTACGGTTTTACCCTGAAAGTCAAACGCTTGGACTGTTTTAAAGTCAACCATGATTAAAGCTTGGCACAATAGTTAACTACCCGTACCAACTGGTTTACGTAAGAGGATTCGTTGTCATACCATGCGTAAACCTTGTAAAGCTTGTTACCATCCACTTCAATAATGTTGGTTAGCTTGGAGTCAAAGATGGAACCGTGTTCCGAGCCGACAATGTCACTGGACACAATTTCATCCTCACAGTACCTAAAGGAAGCGGAAGCCGCCTTCTTCATTGCTTGGTTAATTTGTTCTACGGTAGCATCCTTTTCAAGGGCTACACAAAGTTCAACGATCGAACCAGTTAGTACGGGAACACGAAGGGCCATACCGTTGAGCTTACCAGTAGCTTCTGGCACTACTAGGCCAATTGCTTTGGCAGCTCCAGTGGTGGTAGGTACAATGTTGCAAGCAGCAGCACGGGCACGACGAAGATCGCTGTGGGGTGCGTCTTGTAAACGTTGGTCGGCGGTGTAAGCGTGTACCGTTACCATGGTACCGTGTAAGATGCCGAAGTTCTTTTCCAAGACGTGTACCATTGGTGCTAAGCAATTGGTGGTACAGCTAGCAGCTGAGATAATTTTGTCCTCAGCGTTAATAATCTTGTGGTTCACGTTGTAAACCACGGTTTTAATTGTTTTTTGTTTAGCTGGAGCGGAAATAATCACGCGTTTAGCACCAGCTTGCAAGTGCAAACTAGCACCTTCTTCACTAACAAAGCGACCAGTGGATTCAACCACAATATCAATATTGTGTTCTGCTCATGGCAGGTTAGCAGGATCTTTTTCACTAAACACTAACACCTTCTTTTTGTCAATCATTAAGGTGTTGTCCTTAGCTACTACCTTTTTCTTAAACTCACCGTGAGCGGAGTCGTACTTTAGTAAGTGTGCTAAAGTATCGGGATGGGTGAGGTCGTTAACGGCAACGATCTCAATATTTTTTTGGCTTAAAAGTGCACGAAAAACAAGGCGTCCAATACGTCCAAATCCATTGATAGCAACACGGATAGTCTTACTCTTTGCTAGCATAATCCTCTAATTAGTTTATATTTTACTACTTTAACCTATGGCGAGTAATTCTAGTTGCCAAAAAGCTAAAAGTTGTGGACTGGAAATTTGCTTAAAAAAGCAAAAGTTTTGGTGGGTAAAGGTAAAGCTGTAGTGCGGATTGGTTCAGTACACCAAGTGTCAGTTGTTCACCATTAATAAGCAACTAAGTAAGACAAAAAGCCCCAGAAAGACTATCCCACCCCAAAGGTTGTACTTTTGGAGATAGTGCGTTTTATTAGGATTGTCCAACTGGTAACCCTTGGCTTGTAGCGCAAACGCGACTGCCTCGGTTTTTTTGACCGTTGACAATAACACCGGAATAAAGAGCGTTTTGAGTTTGACTGGATTTAAAAAGGCTAGCTTACCGTACTTAAAACCACGGATTGCTTGCGCTTGTTTAATCCGTTTTAATTCACCCTTAACAATGGGTAACAGTTTAAACACCAGTGCAAATAAGATGCTTAACGGTTGTGTTTTAATTTTGAGGTAGCGCAAAGGGGTACACAACCGTTCGACCCCAAACGCTAACTCATAAATCGAAGTGGACGCAGTTAAGAGAAAGGACGTGGCAAACAGCATGCTAATTCGTAGTGCTATTACCAATGAACGTAGTAAAGAGCGGGTATTGACACTCCACCAACTCACCCCACCAAACTGTTCACCGCCTGAATAAATAAAATGACCTAAAAAATTTTCATTTTGACTGGCCAATGCACTCGAATTCGGTAAGAAAATAAAGCCGTTGACGACAATGTTAAACCAAAAGAAGAATAACAGTCAGCCAAAGATAATGGCAAACGTTTTGAGTTTGCGCTCCACTACAATCACAAGCGCAAAGAAGATCGCATTAATAATAATTAGTCCGTGTAGCCCTAACGGGAGAAAGGCCACCACAAGGCTAATAATTCACCACCACAGCTTTAAAACGGGTGGTAACTGACTTAAAAAGTTTTTACTGGTCATGGAAGAGCGGTTTGAGTTGATCAATCAGATCGTGTACGGTTTTAATGGCTAAGGGATCGTTTAACTGCACACCCTTTGCTTGTAACAACTGAATGGTTTGCACCACAAGTGGTGGGGTAATTTCGTACCGTTGTAAAAGCTGCATGTTGTTAAAGAAATCTGCAACACTCATTTGGTCGACAATTTGACCAGCCTCTAATTGAATCAGACTGTCAGCGGTTTCGTAAATGAAATCAACGTCGTGACTGACAATCATAATGCGCGTGACCTGTTTTAATTGTTTGAGTAAAGCACTTTTAAGTGCTTTACCCTCCTGGTCTAACCCGACCGTTGGTTCGTCCAAAATGAGAAATTGCACTTGCATGGCTAATACACTCGCCAATGCCAAGCGTTTCTTTTGTCCGCCACTCAACTCCAAGGGGTTTTTAATTAGGGGAATGTGGTCCAAACCACACATCTCTAAGTACTTTTTGGCAAAGTTGACATCACCCTTTTGACACAAACCGAGGTTAATCGCTCCCGTTAACACCTCTTCTAAAACGGAATCACAGAAAAACTGTTCTTCGGCTTTTTGTAACACATAGCCAATGCTGTTTTGGAACTGCTTTGTGGCACGGTTATGTTTTTGGTTGGCAAAGTAATAAAAGTTACCACAGTTCAAAAAACCACAGGTCGGATTTTCAACTAAACCTAACTGTTTAATTAGGGTCGTTTTGCCACTGCCACTAGGACCAAAAATAACGGTAATTTGGTTAGGTTCCACACTACCGGTAACGTTTTTAATAATTTGACCAGCATTGCTTTTATCAACAAAGACACTAAGGTTGTAAAAATACAGCGTACTTTGCGCTAAATCAACTAACTGCTGGTTAAACAGCGGGGTGTGTTTTTTAGCTGTTAAATAATAAAAAGGGTGCACCAGTTTATCAAACTGTTTGCAGTACCAAAAGTAGTGTTTAATGCTGCTTTGAATCAGTTTGCGGTTAACCTTATTGGCTAAACCACGGTACTGTGGTGCAATCAGTGATCAAATTATTTGAGGTGTTTGCATATTCAATCAACAACGTCTTGCAACTCCTCAATTGGACCCTTCATTTCGTGGTGGAGATGATCCGCTACTTCATGGGCCAAAAGTCATGGAAACGGGAGGGTAAAGTGGTGCTGGTGGAACAAGTGGAGCTGTTTGTATATGGCAGCGGGTGAAAACTGTTTTAACAGCTGTCCCTTACTTAAAAAGATAATTTCATCCGCTAAGAAGAGGTCATTAAAGTCATGGGTAACATTAATAACTGTTTTTTGCTGTTCTTTAATTACCTTAAAAATGAACCGCTTTAGTTTGCTAGCTAGCTTACCATCCAACATACTAAAGGCTTCATCTAGTAAATAAAGTTCGGGTTTCACCGCCAGTAAACAAGCAAACACGGCCCGTTGTTTTTCGCCAAACGACAGCTTACTGAGCGGTGTGAACTGTTTACCTTGTAGTTCTACAACCGCTAAAACATCAGCAATCACTCGGTCCATTTGTTTAGGATCCACCCCGTGGTTTTCCAAGCTAAAAATGAGCTCTTGGTGCAGCGTATCACCCAACAGTTGCATGTCGGGATCTTGCAGTAAGATACCAATTTGGCGAAAACCAACTTCTTCCAAGGGTTGACCCCTAAAAAAGATTGTTTGTTTCGGTGCTTTTAAAAATCCGCCTAACAGTTTGACCAGTGTCGATTTACCCGAACCATTGTGGCCAATAATGGCTACATGGGTGTTGGGTTTGAGGCTAAAATTGAGCTTTTCTAAGGCCGGTTGACTGTGGCGCGGATAGGTAAAACTGAGGTTGGTAACGTTGAGCAATTTAAGTTTTTATTTTTAAATTTTAAAAATAAGACTAATTGAAAAAATGGTTTAAAAAGTGCTTAAAGACGTCTATAAACCATTATTACAGGTCAAATAAGACATAAAAAAAGAGCAGTTTAACTGCCCTCTTTTTATTGATCTACTGCAACAGTAATTATTGTGCTGGTTGACCTTGATCCCCGTTAGGGTTGTACTTCTTGGCCATTTCTTCCCTTCACTTCTTGAAGTTTTCGATTTCGGCTTTTAACTTGGTGTAGTCATTGGCCTTAATTGCTGCATCAATACCACCGGTAATCTTGTCGAGTTTTTCCTTCTCTTCTTTAGGGAAGTCCTTCGCTTCGGGACTTTGGAGAATCTCCTTAATCGTGCTCACGATGCTTTCACCTTCGTTACGGAGCTCAATGCGTTCACGAATCACATTGTCACGCTCCTTGTTGGCTTCCGCATCACGAATCATCTTTTGGATTTCCTCTTCGGACAAGTTGCCGTTGTCACTAATAGTAATACTGTTTTCCTTTTGAGTGGTTAAATCTTTAGCCTTCACGTTTAAGATCCCGTTGGCGTCCAAGCTAAAGGTAATTTCAATTTGGGGTTTACCCTTGGGTGCTGGTTGGATGCCCCCTAAGTTAAAGCGACCCAAAGACTTGTTGTCACGTGCCATTGGGCGTTCCCCTTGACAAACCACCACATCCACTGATTCTTGGTTGTCTTGCGCTGTAGAGAAGATTTGACTCTTACTTACAGGAATGGTGGTGTTACGCTTAATTAACGGAGTTGCTACACCACCAAGGGTTTCAATCGAGAGCGTTAAAGGAGTAACGTCCAACAGTAACACGTCCTTTACATCACCGCGTAACACACCCCCTTGGATGGCAGCACCAATGGCTACCACCTCATCCGGGTTAATTGAGCGGTTTGGACTGTGTCCTGGTACCATTGATTCCACTAGTTTTTGCACTGCTGGCATCCGGGTCGATCCACCCACCAACAAGATTTCATTAATTTCTTCTGGTTTAATCTTGGCTTCACGTAAAACATCCGAAATTGGGTTGCGAGTACGTTCTAGTAATTGTTTGGTAATTTCTTCAAACTTAGCACGGGTTAGCTTAAGTTCAACGTTTACCGGACCCTTTTCGGTAACCGTTAAGAACGGTAAAGAGATAATTGCTTCTAGTTGGGCGGAAAGTTCAATCTTAGCACGTTCAGCCGCTTCCTTTAAGCGTTGCATAGCCATCTTGTCATTGGACAAGTTAAGCCCATTGTGTTCTTGGGCAATGTGCGCTAAGATGAACTCAATAATCTTGTTGTCCCAGTCATCACCACCCAAACGGTTGTCCCCAGCAGTGGCTAATACTTCGAAGGTACCTTCAGCAATGTCAAGTAAGGAAACGTCAAAGGTACCACCACCAAGGTCGTACACCAAGACTTTCATTTCTCGGTTAGACTTGTCGATCCCATAAGCCAATGCAGCGGCGGTAGGTTCGTTAATAATCCGCTCAACGTTTAAACCAGCAATCTTACCAGCGGTTTTAGTAGCGTTCCGTTCTGCATCGTTAAAGTAAGCAGGTACGGTAATAACAGCACGGGAAATCGTTTTACCAATCTTCTTTTCCGCATAGTCCTTGAGGTAGCTCAAGATTTGCGCTGATACCTCTTCAGGAGTTAACTCTTTGGTAGAACCATCAGGATTCTTAACGGTTACCTTATTGGAGGTACCCATTAAACGCTTAATGGAAACAATAGTATTAGGGTTAGTTACCATTTGCCGTTTCGCAGCATCACCCACAATAATTTCGTTGTTCTTGTAAGAAACAATCGAAGGGGTGGTGCGTTTACCTTCAGGGTTTTCCAACACTACTGGGCGTCCATTCTCCATGACCGACACACAGGAGTTAGTGGTACCAAGGTCAATGCCGATAATTAAGCCGTTATCTGTACTCATATGTTTATGTGCTAAATATTATAAACAAATCGTTAGCACTTTTAGCGTTTGAGTGCTAATTATGTAACTTTTTATTGTTCCAAAAGTGGTGGTTAATCAAGTTAACTGTTGGTAATACTGGTTTGGGGTGTTTGAATTCAATTACTACTTTAACCACTCAACCAATAAAACAGAGACTAAATAAGATAGCTGGAATATAAACAGAGAGAAACCTTCACAAAAAGACCCCTTGCTTTACTTGCGATTCAATACCAACAGCCGATTTAAAGGCATTTAAAAAGCTTGTCATTACAAACTGGGTAGCACCTTCCCCACTAGGTACGGGAATAAAGTTAGAAGCGGTAATGGCAATGTTAGTGATGTTGAAAATGTCAGCCGTAGAGTACTGGTCAATAACGTTGTTAGTTGCGTTTTCCTTTTTGACAATGGCAAAAACAGCAAACACACTAAAGTAACCCACCACTGCTATTAAGATATTGGCTAGTAATTTGAAGATCGTCAAGCCTAAACGTTTAATTTCTAAACCATACAACTTATTAAACTCTGCCTTATCAATAAAACGCTGGTAAATCTGTTCCTTAGTTAAGTAAGGACGCTTAATTCACTTACGAAACTGGTTTACCCCGGAGTAAATTAAGACATGCATCCTTTTACTGTAGGCAATAAAGATAAATAAGATGGCTACCACCACATCAAAGATCATCCCCGCAAAACTAAATCAGTACGACACAAACCCTTCGTGGTTTTGTTCTAACAAAGCATAGTTTTGGGAAAGAACAAAGAAAGATGGTCAAGTAATTAACGCTTGGGCAAGGTTCCAAAAGGCGCCCGTGGAAGTAACTAATAAGACCGCTGTCTGCTTCTTCATGCCCTTTTTAATGAACCAGTACAAGCGAAAGGGATCTTGACCCAAGGAAAGTGGGGTGACAATTTGAAAGAACTGCACTACACAAGCAAACAGAACCCACTCCCATCAAGGGGCATGGAAGCAAAAGCGGCGTGCTACTCATCAATTAATGATTATGTTTCAAGCTAGGGTAACCACAATCCCCAATACTATTAACAGTATTCAGGGTCAGTTAGTTCTGTTAATGGTGTTAATAATCGTTTTAACATCACCAGTTTTAATGTCTAAAAAGAAGACGGTCACCAATACACTAACAACCACCAAAAAGGCAATAAAAAAGCTAAAGGCAAGAATATTTTTTCTAGTAAAAAAACTAGCGCTTTTAGGGGTTGACATTGACCTTTGCTATTTGAGCAATTTTAACCCACTTTCTATCCGTTTATCACCAATATTTAACTGCGGCTTATTAATGGCACTAAGTGCCAACTGTTGTGTGCCCTTGTCTGCTGCTTGGAATAAAAGCAGCGCTGCCACAACATCACTGGCAATTTTATTGTTGGTACCGCTTGTAGTTGTTAGACTCAAGCTGTCAATGTCTTGGTAAGTGAACTGGGTGTAGGTGTACTTGTCACTACCATTTTGGTAAGTGAGGTAGTTGCCAGTTCCACTTGGAGTAGAATCCCTTTGGCTCTTTTGGGCACCATTACCAAAGTAGTTTTGGTACTGGTCCAAGAGGTCTAATAACAGTTGACGCTTAGCTTGGAAGTCCAAAGTTGGGTTGCCAACGGCATCCACTTGGAAGTAGGTCGCAATTGTTTTGCCACTGCCACCATTAGTTGTGGAAGGATTTAATCCACTTAACTGGACTGCAATCCGGTCTAGTAACAATTGGTTATTAGTCCCTTGAATGATCTTTTTGAGTTGATCCTTGGAACCGTACTGTTGCAAGGAGGTTTTCGTAATTTGCTGCAAAATGGCATCACGGGAACTAGCATCGAACATCGAACTAGTGCTGGTACTCAAACCAGTAAAACCATAGTGATCAGTTGAATTTTGTTGCATTTGTTGTTTTTGTACTAATGCTAAGCTAGCAACTTGTTGAGTGGCTTGTACCGTTGGCTTGGTTGTTGTTTTGTAAAAGAAGTTATTGGGATCTAGTGTTTGAGTGGATTCTTTAACTTCGTTAGCGGAGTTCTCATAGTTGAAGTAGGAACCAAATACGCCTTGGGGATTAGAGAAGTTAGTGGTTTCAGTGTTCTTGTAAGCTATAAAAGCAACTTCACCAAACTTAAGGTTTGTTTTTAACAGTGACTTAAAGTTCTTTAATTTTTGGTCCACTAGCCACTGTAAGGTATAGATAAAACGGTAATGGTCAATGACCGCTTTGTAATCAACGTTAAAGGACTTTTCGTCGATGCTAGCTTCAAAAGCCAGTTTGAAAAGCTCATCAAACTTGTTGTCCTTCGGGTTACCGTAAATCGATTTGTCCGTGAGTTTAGCCCAAGTAGTGTAGTAATAGTTACCGATAACTTTACTTAATGCATCGGCATCACTGTTGGTTGTGGAACTACTTTGCATTCCACCATTATTACTGGACTTTTCCTGAATGTCTTTAAACAACTTTTGTGCTTTCTTAAATAAATCGTTATTATCGAACGCTTGTTTGAGCAACATAAACGAAAGCGGGTTGACTTTGTCGCTCTGTAATAAGCTAGCTAAGCTCCAGTTAGCACCACTAGAAAGTACTTTGTCATTGTTAACGTTTAACAAAATGACGTTACTGTAAGCTGGTCGGGGTGCTAAAGCAGGTTTGAGTTTGTTTTTAACAAAATCATTTACTTTAGTTTGGATGTTTTCCAAGATTTTGTCCCTAATCTTGTCAACGTCAGCAGTTTTAGTTTTGAGCTTATTGCTTTCTTCACTTATGCTTGTTTCTTCTTGACCAACTTTAGGTCACAATGTTTTTGTGATTAAGAAGGTAAAGTAGTTGTTTAACTTTTCGTAATTACCGTTAGTATTAACGTAAGGTAACTTGGCTGACAAACCGTTCAACCAAGGGGTTGGATTGGCTTGGTTGACGTTTTGGTTAAGATTACGGGCGCGCTCTAGCAACTTGTTATTGAGCGCTTTCATTTGATCAAAATAAAAGCTGATCATTGTGGTTGCTAAAGCCACCGGCGCAAATTCTTTAATTAACTCTTTATAAGGATTAAAAGCTTCAGTTAAAGCTTTGCTAGCTTTATCCTTTTGGTCAAGACTAGCGTTAACTAAGGCGTTGGCCAAAATTAGTTCCCGGTGTTTATCAAACCAACTTTTTAGCGTTTCGTTTAAGGGAAAACTAAAGGCAAAGTTAGTACTGTAGAAAGTGTTAGTTTTAGCTTGAAAGGCACGAAACTTTAAGAATTGTTTTTGCTTGTCAAGGTCTCTTAATACTTTATTACTGTTACCTGAACTAGATTTAGTTAATAAGGTTAAACTTTGCTTAGAAACTGATTTTTTATTTGTTGAATCTTGTTTTAAATAATAGCTCCCACCATCAAGGCCGAAAACAGTTACTCCGGTGCTATTACGCACAATCCGGAATTTAATGGGACTGTAACTGTTTTGATCAGTTTGCTGAACCTGCCTTAAATTTATAGATCCAGATTTTAAGGCAACTAAAACTTCATTAGAAGCGCTATTATTTTTCGATTTTGATGTTGTTGAATTAAATGTAGGAATAGCTGCATCTAAAACAACGTAATTGTTTTGTTGGCCCTTAGAATTGTTAGTTCTTAAAACAGCTGTTTTCGCTTCCATTTTTGTTGCAGCTGCTTTCTTTACCTGCTCCGCTAACTTACTTAAGTTAGTGCCTTCGCTTGTGTTTTGAGCAAAAATGTCATCTAAAGCGCCAGCAATTGGATCTGAGTCACTTTGTTGTTGCTTACCTTCTTGATCACTGTAAAACAGTGCGTTAGTTTTGGAAGCTTTTTCTACGTCCTTACTTTTATCGACAACAATCGTGTTGTTAAGGTCTTCTTTGAGCTCCTTAATTTGCGGCAATTCGTCATTATCTTGCTCAAAAACTTGCAGTTTTAAATTTAAAGCTGCACCTAAGTTAGCCGCTTCTAACTGATCGACAATGTTAGAAGCGTCTACTAATTGCTTCGTTTCGTTAGTATCTGATAAATTTAAGGGAAAATCAAGAAAATCACCCTTATTTGATTCATTACGGTATTTGAGCAGACCAGTTTTTTGGCTGTTTGTACCGCTTTGACCGATATTATTATCAGTAATAGCGCCCTCACCTTTAACTAACTGCTTTCAGCGCTTATTACCAACGTTTTGGTTGGGTGCAATGTCTTTTTCTTCGGCAAAGAAAGGAAAGGCATAAGAAGGTGTTAGTTTATCCTTTAACTTCTCTCTGTTATAGATCTGACCTAACCCTTGACTGGGAGCAGAATACTTATAGTTAACTTGACTAATTAAAGTGGGATCAGTGTACTCCACCCATTGGTCAAATACTTGGCTTTGAATTTTGGCGAAAAAGTCATCATTAGTTTCACTAAAACGTGGGGCACTAAATTCAAAGTTTTTTCAGCTTTCTTCCTTGTGTAGTTCATCATAAGTAAATGGACCAGTAGAAACTTGTCCATCTTTTTTGTAATTGAGGTAGTTCTTTTGGAAAACCCGACTAGCAAAGTCACTAATGAGCTGTTCAAGCAGCTTTTGCGCTTTCCAGGTTGCTTCACTACCACCGGCGTTATCCAACAAATCTTGTTGCACAAAAAGTGATTTATTGCGTGATGCTGATACCACTTTATCGACTGCCGTTGTGTACTGACTGTCAACATTGGACTTAGTGTCTTTTAAAAACTGGGTAATCTTTTTGTCTTCGTTGTTTTCGTACCAAGCTTCTAGTGCTTTTAAAAGTGGTGCAGCGATAAATTGTTTGTTGGCCACTGGATTTTTTAAAGCCGTTGTTAGGGCACTTTGTAAACTACCCTGTTCACCATTGAAAAACTGACTGGAACGCGCTTTAAAAACGGTTTGTAAAGCCGAGTTGGAAGGGGTAGCACAAGCAGCTAAAATAACACTAGTAGACAGAAAACCGCCTAATGTTAAGAGTCAAAATTGGGGTTTTCTTAGAAACTTTTTCATTAACGCGGTTTTTGCTCTCTAAAGGTTAAATTTTTGCTTTATTTTGGCACTTTAAAAGCTAGTTTTTACCTAAAAATAAGTATTGATAAGTAAGAAAAACTGCTTTAGTGCCCACTGGTGCAACTAGCTTAATCTCTTGGCTTAATTTTGTACATTTTAAACCACGATGGCATGACAAACTTACAATTTCAAACGTTTTTTTTTTTTTTTTTGAAAGAAATTGATTGCTTTAACTAACCTGCTTTTATCTAACTATTTAAGTAAATCTATTCCTGTTTTAATCCGCTTATCACCAATTGGTAGTTGTGGTTTATTAATAGCACTCAAGGCTAATTGTTGGGTACCTTTATCCGCTGCTTGGAAAAGTAAAAGGGCAGCCACAACATCGCTAGCAAGCTTACTATTTCCTTTATCACTCAAACTTAAGTTATCTATATCTTTATAGGAGAACTGAAGGTAAGTGATCTTGTCACTGCCATCTTGGTAAGTTGAATAGCTTCCATGTCCACCTTTACCAGAATCTCTTTGTGCAGCCTGAGTATTTGTACCAAAGAAATCTTTGTACTGATCTAAAACATCTAATAAAAGTTTTCGTTTTTGTTGAAAGCTAAGGGTTGGGTTGCCAATGGCATCTAATTGGAAATAAGTAGCAATATTTTTACCCTTTCCATTATTACTATTTCCAGTGGTTGAAGGATTTAATCCACTTAATTGACTAGCGATTCGGTCTAATAAAAGTTGGTTATTAGTCTTTTCAATCAAAGTTTTCAGTTCTTGCTGCGAACCATATTGTTGCAAGGAATTCTTGGTTATTTGATCCAAAATGGCTTGCTGCGTAGCGGCACCTAACATTGAACTAGTACTGGTGTTTAGCCCGGTAAAACCATAATGATTACTATCTTGCATGGTACTTTGCATTTTTTGTTGCATAACCATCATGCTTGCACCATTTTGTGCGGTTTTAACTTCTTCTGGTTTGGTAGTTGTTTTATAAAAGAAATTATTTGGGTCTAAGTTTTGCGCAGCAATCTTAACTTCATTAGTTTCGTTTTCGTAATTAAAAGCAGAACCAAATACACCTTGCGGATTGGAAAATTCTTTGGTTTCAGTGTTTTTATAAGCAATGAAAGCAATTTCACCAAACTTTAAATTTGCTTTTAATAAGTCCTTAAAGTTCTTTAAGTTACCGTTAACTAGTCATTCCAAGGTATAGATAAAGCGGTAGTGTTCAATGACCGCTTTGTAATCAACATTAAAGGACTTTTCGTTAATACTATCTTCAAAAGCTAATTTAAATAAGTCATCAAATTTGTTGTCCTTAGGATTTCCATAAATGGATTTATCAGTCAATTTAGCTCAACTGTTGTAGTAATAGTTGCCAATTACTTTACTCAAGGCATCAGCTCCTTCACTACTCGAGGTATTGCTACCTTGCATCCCACCTGAACTTCCACCATTTGTTTTACTTTGAATATCTTCAAAAAGTTTCTTAGCTTTCTTAAACAAGTCGTTTTGGTCAAACGTTTGTTTGAGCAACATAAATGAAAGTGGATTAATTTTGCTCGTATCCAATAAGTTAGCTAAAGCTCAATTGGCGCCCATCGATCAAACCTTATCGTTGTTAACGTTTAACAAAATTACATTACTGTAAGTTTGTCGTGGTGCTAAGGTTGGTTTAAGCTTGTTTTTAACAAATTCTTGAACCTTCTTTTGAATGTCTTCTAAGATTTTATCTCTAATTTTGTTAACGTCTTCGGTCTTAGTTTTGAGCTTGTTAGTGCTACTATCTTTAGATAATGAAGCGTCTTCATTACCGACCTTCTTCCACATGTATTTAACAATTAAGAAAGTGAAGTAATTGTTGAGTTTTTCGTAATTACCATTTGTTCTAACATAAGGCAATTTAGCTGATAAACCATTTAACCAAGGTGTAGGATTGGCCTGATTCACATTTTGGTTTAATGCTTTAGCGCGCTCCAAAAACTTATTGAACGCGTTCATTTGGTTAAACAAGAAGTTAATCATGGTTGTGGCCAAACCAATTGGTGCATATTCTGCCAGTAAATCCTTGTAAGGCTTTAAGGCTTCAGTTAAAGCTTTGTTTGCTTTATCCTTTTGATCAAAATTTGCCTTTACCAAAGCGTTCGCTAAGATTAGTTCGCGGTGTTTATCAAATCAACCTTTCAAGGTTTCATTTAACGGAAACGAAAAGGCATATTTGGTGCCGCCTTGTTTGAAAACTCTATTTTTAGCTTGAAAGGCACGAAACTTCAATAAATTTTTTTGAAGACTGCTATTAATCTCTTGTTCTTGATTATTATTACTTTGGTTTTGAGCGAGAGAAGTGGCTATCGTAGAAGAGCTACTTTGTTGTTTATTGGAAGATCCAACAACGTAGTAAAGCAAGCCTTCAAGAGCCAGCAATTTTACACCAGTACTATTTCGAACAATGCGCTGGTTTAAAGGCGTTGTACTACTTTTTTGTTGAGTTAAATTGACGCTAGTAGAAGTCAAATTAACTAAAGCGCCACTACCAGAAATAGTTCCACTGCTTTGTGGTGATTGTGAAGAAGTTTCAAACGCCGGGATCGCAGCATCTAATAACACATATTTTTCATCAGATTGTGCCATGTTTGTGGTTTTGAGACTAACCGCATTTCTTAGTTGTCATGTCTGACTTCTAGCTTCCATTTTAGTACTGTCAGCTTTTTGTAACTGTTCAGCTAGCTTACCTAAATTACCACTATCGCCATTTTGACCATTAAAAATTTCCTTTAATTGAGTTTTAGGTGAATTTTTGCTTTCAGCACTTCCTTGTTTTTCGCTACTATCACCATTATCTTTGTAAAAAGTACTGTTAGTTTTAGAAGTTTTCTCTAAGTTATCAGATTTAGCTACAATGGTGTCATTCAAATTGTCAGGAAGCTTTTTAATAACAGGCAGTTCATCAAAGTCCTGTTTTAAAAAACTGAGTTTAAGGTTAAGTGCTGCTCCTAAATCGGCTGCTTCTAGTTGATCAACAATAGTAGTGGCATCAACTATTTGTTTAGTTTCACCAACATCAGTAAGAGTATTCGGGAAATCAAATAAGTTTCCGCTTGATGTATCACCTCTTCATCCAAATAACCCTTTTGTAGAAGAGGAACTATCGTCGTCATCTGAACTCTTCTCCTTGCCATCATCAGAGCCCTCGCCTTTAATTAATTTCTTTCAGCGCTTGTTACCAACGTTTTGATTTTCCGGAATATCTTTTTCCTCAGCAAAAAAAGGGGAAGGCATAGGAAGGAGTAAGTTTATCCTTTAACTTATCTTTGTTATAAATTTGACCTAAACCTTGATTAGGAGCTGAATACTTTAAGGTAACTGAGCTAATAATAGTTGGATCGGTGTACTCAGCCCATTGATCAAAAACTTCGCTTTGAATCTGCGCGAAAAAATCATTATTAGTTTCTGGAAAATTACCAGCATCAAAAACTATATTCTGTCAATTACTATTTTTTGAAATCGTGTCGTATAAAAACGGACCGGCTGATACTTTGCCATTTTCCTTATAAGACAAATAATTCTTTTGAAAAACTCGTGAAGCAAAATCACTAATTAGCTGTTCAAACAGTTTGCGCGCTTTTCAAGTGGCCTCACTACCACCGCTACTGTCCAATAAATCTTGTTGTACAAATAAGGATTTATTGCGTGGTGCTGAAACTACTTTATCTACAACGGTTTTGTATTGGTTATCAACATTAGTCTTGGTGTCTTTTAAGAATTGAGTAATGTTTTTATCTTGATTATTCTCGTACCAAGCTTCTAATGCTTTTAAAAGTGGAGCAGCTACAAATTTTTTGTTAGTTTCCGGATCTCTTAAAGCGGTGTTTAAAGCGCTTTGAATGGTACCATGCTCACCGTTAAAAAATTGGTTGGAAGTTGGTTTAAAAACTGCTTGCAGTGCTGAGTTTGAAGGTGTTGCACAGGCACTGAGAATAATACTACTAGTGGAAAGGCCACCCAACAATAAAAGTCAAAACTTGGGTTTTCTTAAAAAACTCTTCATTTAAAATAGTGCCGTTTGTTCTAAAAAAGAAAAAAGCAAGCTTACTTTTTGGCTTAAGGTTTTTAAATTTTAAAGCACCTCGATATTTTTGAAAAATTATTTCTAAACTTTTTTTTTTTTTTTTGCAAAAAGTTGTTAGGCTTAAAACCAATCTTTTAGCTCACTATTTCAGTAAATCGATCCCTGTTTTTATGCGTTTATCGCCAATTGGTAATTGTGGTTTATTGATGGAACTTAAAGCCAGTTGTTGAGTACCTTTATCGGCTGCCTGGAATAATAAAAGCGCTGCTACAACATCACTGGCAAATTTGCTGCTAGTTCCGTTTGAACTACTCAAACTTAAACCGTCAATATCTTTATAGGAAAACTGCAAATAAGTGATCTTATCACTACCGTCGGTATAGGTTAAATAGTTGCCAGTTCCACTCTTACCAGAATCTCTTTGAACTGCTTGTGCATTATTACCAAAGAAATCTTTGTATTGATCTAAAACATCCAATAAGAGTTTTTGCTTCGCTTTAAAGTCCAAAGTAGGATTGCCAACCGCATCTGTTTGGAAGTATGCAGCAATTGTTTTTTGGGTACCACTGTTGCCACTTGTATTCGGTTTTAAGGCTATTAGTTGGTTGGCAATTCGGTCTAATAATAATTGATTTTTAGTTTCGCCAATGATCTTTTTCAGGTCAGCTTGAGAACCATACTGTTGCAAGGAAGTTTTGGTTATTTGATCCAAAATGGCTTGCTGGGTAGCAGCACCTAACATCGAACCACTACTGGTACTCAAACCAGTAAAGCCATAACTGTTAGTTTGCTGATTGTTCATCTGCGTATTTTGAACCATCACAGCTGTATTAGCACTTTGAGTGGTTTTCGCTTCTGGTTTAGTGGTGGTTTTATAGAAGAAACTGTTGGGGTCTATAATTTGCGTAGCACTCTTAGCCGCATTCGTTTCGTTTTCGTAATTAAAGTAAGAACCGAATATACCTTGCGGGTTAGAGAAGTTCTGCGTTTCAGTATTCTTGTAAGCAATAAAAGCAATTTCACCAAACTTGAGGTTTGCCTTTAATAAATCCTTGAAGTTCTTTAGGTTTTTGTCTACCAACCACTCTAAGGTATAGATAAAGCGGTAGTGTTCAATCACTGCCTTATAATCTACATTAAAGGACTTTTCGTTAATACTACTGTCAAAAGCCAATTTAAAGAGATCATCAAATTTGTCATCCTTAGGGTTGCCATAAATTGATTTATCGGTTAGCTTAGCTCAAGTGTTGTAGTAATAGTTGCCAATGACTTTGCTCAAAGCATCGGCATCATCGTTGGTGGTTGTACCACTTTTACCACTTCCACTAGTTTTTGCTTTATTTTGAATGTTTTCGAATAAAGTCTTTGCTTTTTTAAACAAACTGTTTTGATCAAACATTTGTTTGAGCAGCATAAACGAAAGCGGGTTAACCTTTTTCGGATCTAGTAAAACTGCCAAACTTCAATTAGCGCCAGCAGACCACACCTTATCATTGTTAATGTTTAACAAGATTACGTTACTGTAAGCTGGTCGGGGTGCTAAGGTAGGTTTGAGTTTTTGGACAAAAGTTTTGAGCTTACTGTCGATGTTGTTCAAAATCTTTTCCCTAATCTTGTTAACATCTTCAGTTTTAGTTTTGAGTTTGCTTTTTTCTTCACTGACATTAGTATCCTCAGTGCCCACCTTAGGTCACAAAACTTTGGTAATTAAAAAGGTAAAGTAGTTGTTTAACTTTTCGTAATTACCGTTAGTGTTAACGTAAGGTAGTTTAGCCGATAACCCATTTAACCAAGGGGTAGGATTGGCTTGGTTAACATTTTGGTTTAAGCTCCGTACTCGTTCTAACAGCTTATTGTTGAGAGCTTTCATTTGGTCAAAATAAAAGCTAATCATGGTGGTAGCTAAACCAATCGGGGTGTATTCACTCAATCAGTTTTTATAAGAACTAAAAGCATCCACTAAGGCCTTACTTGCTTTTTCCTTTGTGTCTAAAGTAACTTTAACTAAGGCGTTCGCTAAGATCAATCCGCGGTGTTTGTCAAACCAACCTTTTAAAGTTTCGTTTAGGGGAAAACTAAAGGCAAAGTTAGTGTTATAAAATGTGCCAATTTTGGCTTGAAAAGCACGGAACTTTAAGAATTTCTTTTGCTCTTCTTTATCAATCTTTTTGGCACTACTAGAACCGCCATTGCTACCTCCGCTACCGCCTTTTATGAATTTAACAAAACCATCAAGTCCTAAAACTGATACGCCAACACTGTTACGCACAAGTCTCAGGCCAAAAGTGTTATATTGATCTTTAGTTTCTCCTTGCTGTTGCATTTTTAGTTAGGCAACTGTAGATGTGTTTAAAGAAATTAAGGTACCGTTATGAAGACTCACTGAACCTTGACCCTGGGTGCTGACTTGCTGTTTAAAGGTAGGAATACCAGCATCTAATAGAACATAACTGTTCTTTTGTTGAGTTTGCGATTTATTTCGCAAAACAACAGTGCCTCTTGCTTGTGGGGTTTGCGTTTTAATTTCCGCTGTCTTTTTAATTTGTTCTGCTAACTTGCCTAGATTAGTTTTTTGTTCCACGTTTGTGGCAAAGATGTCATCTAAAGCTTGGGCAATGGGATCGGTATTTGTGGTTCCAGAGTTTTGATTATCCTCATAAAAAGAAGAATTAGTTTTGCTCACATTTTGTGCATCACTACTCTTATCAATCACAATCACGTTGTTAAGGTCATCTTTGAGCTCCTTAAATTCGAGTAGCTCATCCTTCTCTTGACCCAGTCGGTCTAGTTTAATGTTGAGCACAGCGGCTAAATCAGCTGCCTCTAATTGATCGACAATGTTGGTGGCATCCACGACTTTTTTGGTCTCGTTAGTATCTGATAAGCTTAACGGAAAGTCAATTACTTGGCCTCTGTCGGTTCCCCGTACATACTTGGTAAGGCCTTCTTTTTTGAACTCCTTTTGTTTGACTGCCATCTTGTTGTTGCTTTGGCTTTTCTTCAATCGCTTCTTCACCTTTAACCAGTTGTTTTCAGCGTTTATTGCCGACATTTTGACTCCCAGCAATTTCATCCGCTGCAGCAAAAAACGGAAAGGCATAGGAAGGGGTTAATTTATCCTTTAGTTTGTCCTGGTTATAGATTTGCCCTAATCCCTGGTTAGGAGCCGAGTACTTTAAGGTAGTGAAACTAATAACAGTGGGATCGGTGTATTCCGCCCATTGATCGAAAACTTCGCTTTGAATCTGGGCAAAGAAGGCATCATTGATTTCGGGAAACTGGCCCGCATCAAAGACAATTTTGTCCCAATTGCTCGATTTAGAAATTTCGTCAAATAAGAAAGGACCTGATGATACCTTATTACCATCCTTATAAGCGAGGTAGTTCTTTTGAAAGACCCGACTAGCAAAATCGCTAATGAGCTGTTCAAACAACTTCTGGGCTTTTCAAGTTTCTAACCCCCCCCCGGCGCTATCCAGCAAGTCTTGTTGGACAAAGAGGTTTTTGTTTCGGCTAGCCGATACCACCTTATCTACGGCTTGGTTGTATTGCTCATCAACACTCTTTTTGGTGTCTTTAAAGAACTGGGTGACCTGTTTGTCTTGGTTATTTTCATACCAAGCTGTCAATGCCTTTAAAAGGGGAGCAGCGACAAACTGTTTGTTGGCCTCTGGATCTTTTAAAGCAGTGGCTAAAGCGTTTTGTAAACTACCCTGTTCCCCGTTGAAGAACTGGTTCGAACGTGCTTTAAAAACAGTTTGGAGTGCTGAATTGGAAGGGGTAGCACAGGCAACTAAAATAACACTTGAAGAGATAAAGCCACCAAGGGTTAAGAATCAAAACTGGGGTTTTATTAGAAGTTTTTTCATGAACGGCGTTGTTTGCGCTTGGCAAAGAGTTCCGCAATCTGCTGCTTTTGGCGCTTCTTATAGTTAGGCTTGACCTTCTTGTATCCTGCCTTAATCCGCTTAATGGCAGTTTTAAGTTCACTGGATACTGCTGGTTTTTGTTTGCTTTGTTTAAGTTGGGGGAACTTTAAAGTACCATCACGTTCTAAATGAACACTGTTAAACAAAATACCTTTATGGGCAAACTTAGTTAATTTGTGTTGGTTATGCGGTAAATCAAACACATAGGAATTGCCCCAACTGTTAAAACGCGCTACTCTACCAGAACGGTGGATGTAAAAGCTATCCACTAGGGGTAAGTCTCATGAAATGACCACACTAAAGTGGGGCAGATCAATGCCGCGTGCTAGTAAGTCAGATACCACTAGTACCCTCACCGTGTTATTAGCGGCACTAGTAAAGATGTGTTTGCGCTCATTGGGCGTTAGTTGACCATGGAGGCTTTTAAAGCTAACTTGGTTGTGGGCTAGTAAACTAGTGAGTTGTTTGAGTGATTTAGCGTTGGAACAAAAGACCAAGACCTGTTCGTTATGGTGGTAGTTGAGTAAAGCTAATAAACCAGCAAACGGTTCTTGACCGTTGAGGTTAAGCAGAAAGTGTTTCACCAAAGAGTGCTTTCAAGCACGTTTCTTTTGGTTGACGACCGTTAAAGGTGCGACTTGCTTGCGAATTTGCTGGAGCTGTTCGTTAAATAACGTGGCACTAAAAAAGCCAAAGTGAGGTTTATGTTTTTGAAACAGTTGGACACAGTTAATCCACTCTGCTTGGGAACTGTCCTCCAAAAACATGTCGACCTCATCAAAGACAACGTACTTAATCGTTTCGTGGAGAATAATGTCTTTGCCAATTAAGCTCACAATCAGGTGGGCATTACTGTTGAAGTTCTCACTGACCTTTAAACCAGTAAAGCTGGCTTTAATGTCGTTGAGGATCGTTAAAATTTGCCACTTCAACTCCTTAGTTGGTACAAACACCACCGCTTGGGGCTTGTTGAGCGTGACATCAATTTGGTTGAGCGCTGGTAAGAGGTAAGCGAATGTTTTACCACTACCAGTTTCGGCTATGCCAACCACATTTTGACGTGGTCAAACTTTAAACACAGCTTGTTGAATAGATGAAAATTCAACAATGTGCTTCGTTTGTAAGAACGCTTGGAGTGGTTGAATGAAGTGCATAGTGGTTTAAAAACGTCTTACCCATTGCGCACTAATAGAACTAAAGATCCTAAAGTCACCTACTTTAACTAAACCCTTCTTATTACCACTAATTAAGGCGTTAATGGCTTGGGTTTGATTATTAGGATCCAAGGCTTCTAGCGCAATTGCAGCGAGGAATTCTTCGAGGGAGAGGTTGAGGCGTTTGTCTTTGCTGGAATCTTCTTGCTTTAGAGAATCAGTGCCGATCTTCTCCACATCATCAAGGTTGACCTGTTTGACAAAGGTAGCGTGGTATTTGCCACCGTCACTTAAGAATTGGTAGGAAGTGTAGTTCTTTTCCTCTACCTTGTTATCACCAATGTAACCATCAAAGCGGCTAAAGACGTCTTTATAGTTGGTATTTTGGTTATCTTCTTGTGTTTTCTTAATTTGTACTGTTGCAGATCTTGCACTGCGTGCACTTGTATCGCCTCTCTGTGCAGATTGAGTACTCTGCTTAAAGTTAGAATCAACAAAACTCGTTAAACTAGTTTTCTGTTCCGTTAAAGTTTTGTTTTTATCAACCCCAGTAACATCAATGTTGAGGTCACTAGTTAAGTGGTTGTAAAGCGCATCGAACTCGGTTTGCGTTTGGATTCCGTTGATGATGTTCTTTAAGTTATCCATCGAACCAAAGCTAAACAAGGCACCTTTGTAAGCGTTGTTGGTGGTGGATTCGGCGTCGTCAGCTTGTTGTTTTAAAACTAAGGTAGCTTTACCACTTTTAGCTGTGGTAATTAGCATTTGACCACCAACTTTAACGCCTTTTTCTGTTTGGTTAACAAACTGCTTAAACAAACGTCTACTTACCGCTTCGGGTAAACTACCACTAGTGCTACTCGTTACAATACCCCTAAAGCCATAGCGGTTTTTGGCAGTTTTTGTGGTCATGCCACTAGCTTGATTTAAACTCCTTAAAACAGTGGCACTACTTTGTGCTGCGCCAGTACTACTACTAGACTTAGTGTAGAAGAAGCTTGAAGTGTCATCAGCAGCCCGGTTCACTTCTGGGGTTTTACTGTTGGTTCAATCATACACACTCGAACCAAACACAAAGTTCGGGTTTTTGACTTCGGTTTGGGTGATGTCACTATTATTATCACCGCCTTGGCCAGCTGCTTGATTTTGTGCTAGGGAAACGACTGATCTTAACTGAATTAAGCCAGTATTACTTGTGCTTGAAGCGGTACTGGATAAAGTTGATTGTGAATCATTTTTATTCTTTTCGCTATCCACACTTCAAACTAAAAAGGCATTGGAAGTACGCGACAACTTCGACTGTAAGCTTTGCTTATAGTTCTTTAAGTTGTCCTTTAACAACCAATGGGCCGTGTAGAGGTAAGACAGATAATCAATTACATCATCACTATCTAAGCGCTCTTCTAAGTTTAGCGAGCTGTACAGTGTGTCCAAGACACCTTCGATGCCCGTCTTAGTTGAAGAATTTTTATCAGATGAACCAACATGACCAAAGATTGATTTTTGGGCTTGTGATTCAAATAAGTCCAATAAATAGGTTTCTTTAAAAATCTTTCAGTTGTCAGCGGTGAGGTTGTTGTTACCGTTGGTGCCACCAGTACCACCACTTTGTTGACTAAAAGTTAAATTCGTAATATCAATAATCTTTTTAAACGCTTCATCTTCCTGGAAGTAACTGAGCTTAATGCTGTTTTGGAGCTCAGTACTGGTTAAATAGTTGTTTAGAGCTAAGTTAAGTCCTAAAGAGGTACTTTCAGGGGTAAAAGACAACTTAGTTTGCACTAAGATTACCTGGGAGTAGAGTGGGGAAGGAATGGTGAAGACTTTGAGTGCCTCTACATGTTTTTTAGCAGCTTCTTCTACCTTCTTCTTATTATCTTTAAGCTTGCTAACTACTTCTTCAGAGAAGTTTTTAGTTTGTCCTGTATTGGAAGAGGTTGTTTTAACAGTACTAGTTCCTTTTTTATCAACATTATCAATAATGTCCTTGTAATAGTTACCAAGGTCATTATAGTGACCGGTTTTAGCATCTTGTTCGTAAGGTAACTTAGCTGCCAAACCGTTCTGTTCAATCTGGTTATTGCGCTCGTTGTCAATAAAAGGTTGGTTACGTTCTGCTAGCTTTAAGGTAACCTTGTTTTCCGCCACTGCAGTTTCGTTAAAGACTGCTTGTTTATAGTGGGCTTGAACAAGTTCTTTTAAATCCTTCTCAATTGACTTAATACTGTCAGCAAACTTCTTAAACTGGGGATAGCTTAAGAAGTTGTCTTTGTTGTTTGTATCACTAGAAAGATCTAATAAAGCTTCAAATAAGAATAAGATCCGGTTAGTGTCAAAGTACTTTTTGACCTCATCAAATAACTTAAAGTCGTATGTGGTGTCGGTATCCACTAAACCGTACTTGGTTTGCAAAGCCCGGAACAATAAGAATTCCTTTTGTTTAGCGACATCACGACCAATTTCAGTAAGATAATGACTACCACCATCCACGGCCATTAAGTGGATGCCGTCTTTACCACGGGAGAGGATGTAGTCTGGTTGACTACTAGAATCTTTTTTAACTTCAATTAAGTCAACAACCGCCTTACTACTGTTGCCAGTAGTAGTTTCAGTAGAAGATCCGGTGCTACTGCCGGTAGAGGTGCCACCGCCGCCTCCAGAACCTCCGCCACCTGTTTGATTAGCATTAGTATTACCTATTTGTTGTTTATACTCACCAGCAAAGACATCTTTATCACCAAAGATTTTGACTAAGTCAGTTTTGTGGACGGTACTTTGTGTAGTTCCACCACCATCACTGGCAGTTTTTAATTGAATAGTTCCTTCAGATGATGAACTCGCAGCCCTTGCAGCGGCGGCTGCCTGTGTATTATTGTCAACAAAAAAGTTTTCAATAATACTCTTATCCTTAATGAGACTGTCAACTTCTTTACTATCAGCGCCCTTCGACTTTTTCTGCAGTGCTAAATAGCCCTGAATGAAAGCAGCAGAAAAACTCGGATCAAAGGTGTCGAACATGTCGCTCGCCTTTAACAACAATTTGCCACCACTATCACTGGAAAACTTATTGGGAATATCAATCCCTTTGCTCTGGTCGTTTTTGTAACTCTTGAGGTTGCTAGCCAATAAGTTAAAGCCATTCGCTCCCTTAGTGCCACCACTCTGTTGGTTGTATTTGTTAAAGACCTGAAACTGGTAGGAAGGGGTTGGACTTTGAATTACTTTAGTGTTAAAGTAATTATCAAACCCGTCAACGGGAGTTTCATTGGTAAACACGACCCGAGATACCAAGTTGGGGTTTTCATTGGTAATAAACTGGTCTAACAGTTTGTCTTGGATGGCCGCGTACACCGCATCATTGTTAATCTTGAGCTTAGCATTCTTATTAACAAATTTGGGTTGGAGCTTAATGTTTTTAAAGTTAGAGGTGTTTTCAATTAAGCTCTTGGTGGGTTTAGTTAAAGGACCTACACCGTCCTGGACATACTCGACAAAGTTCTTGGCAAAGATCCGTGAGATAAAGTCATCAATTATCTTGTTGTTAACATCACGCAGTTTTCAAGCGGCTTGATTACCGCCAGTGTTGTCAAAGATATCAGTTTGCAGGTGCACTAAATAGTTTTCGCGAAACTGGTTCCGTAGGTTTTGTTCTTGACTGACAAACGCATTATCCGTGTCAGCGGTAAAGCCGTTTAAGCGCTCTTTAATATCAGTATCATAATTGTCGTCATAAAAGGTGCGCAAAACGGGTGCTAAACGCATTGCTAGATACTGGTTAAAACTCTCGCGGTTAATTAGTGACTTTTGCAGAATCGAGCTAAGACTAAAGTCAGTGCGATCAGCAAAAGCACTACTGGGCTTAAAAAGCCCCTCCACAGTGCTGGCTGTAGGCTGCGCACAGGCAACCGCGACTATACCAAAAGTGAGCCCGATTGCGGTGAACATAAAGGGCCACTTGACTTTACGCGTAAACGTATGCATTCGCGTTCAAAATTTGGTATAATTCTAAAATTTAACCACTTTCTTTGACCGGGGATGCGGCTCGAAATTGAAAACGGCTTGGAATTTGTGTGTGATCCCTTTTTAAATGAGCGCGGCAAAATCCTTTTTTTACACGCCTTTACCGGTAATATCACCAATAAATTAAGCTTTCGCACCCACTTTAAAGATTACAGTTTTTACGGGATTAACTTTCCCGGACACGGTAACAGTGTCATTCACAACCAAAGTGAATTAGACTTTAACTTCTGGATTAAGTTAGTGCAACAGTTCTTTAATAAATACCAATTAAAGAACGTGGTTTTATTTGGTCACTCGATTGGTGGCGGACTAGCGATTGCCCTCACCCAAGTTTTAACTAAGGAACAAATTAAAGGCATTATCCTCGAAGCGCCCCTAAACCCGGGGATTCGCGCTACCCCACCAAGTATTATTAGTGCCTTGGTACCTGACACCAACGAAGACTTTGAAGCGGTACAAAGGGCATTGATCTACAACATTGAACAACGCTTTGGGGCGAACTTTAAGGACTTTTGTGCTAAACAAAAACAGAAGATGATCCAAAAGTATGCACCCTTAAAGGTAATGTTGCAACCCGAACAAGCCGAACAACGGCTCCAGTTAATTGATGCGGCTTTTAAACGGTTAAGTTACCCAACCCTGTGAATTCACGGGCAGGAAGATGGCATTGTACGTTACTTGCCCTCGAAGGCTTACCTGGAATCCTTGCACAATCCTTTAATTGAACTAGTGGGCTTATCCAACACGGCCCACACCACTTTCTTTGAGCAGCCCCAGCAGTTTCTCCAACTAGTGGAACAGTTTTTAAACAAGTTAAACAAATAAAAAAACTAACAACTTTAGTTGCTAGATCTCTTATTGTTGATTGATGTTTAAGCGTAGTTACTACTTAACCAAACGCTTGTACCATTCGACCACGTACGATTCGTTAATACCAGCAGGGAGTTCACTACGTTCTGGGAAGCGCACGTATGTCCCTTCAAAGGTCTTGTTGTTGGTTTCCACAAACGGGGAGACCACACCGCTTTCGCTCGCCGCCTTAACAATTGGAATCTTAATTGTTTTGGCCTTCAAGCGTACCTTATCCCCTGGATTGAGAATAATGGAAGGGGTGTCCACGGTACGATCGTTGAGTAATACGTGGCCGTGGTTAACTAATTGGCGCGCACTGCGACGGGTTGGAGCAAAACCCATGCGGTACACAATGTTGTCTAAACGTGACTCTAAAACACGGAACAGGTTAACCGCTAAGTTACCCCGTTGTTTTAATACCAGTCTAAACAAGCGTCTAAACTGTTTATCAGTAATCCCATACATGTATTGCATCCGTTGCTTTTCTTGCAACTGTTGTGCATAACCAGACATAGTGGAACTACGGAAACGGTTCCCGTGTTGTCCAGGAATCGTTTTCCGCTTCTTCCCTTTCGAAAACTCCTTGTTGTTTTCTAGTAACGAAAAACCTAAGCGGCGTGATCGTTTGAAAATACTACCAGTGTATTTCATGCGGTGACAATTTTAAATTATCTTAATAATCAAGATTAAAATCCCCACCGTTTTCCATTAGTTCATCATCCAAGATACTAGAGAGGTTAGAGGTTTTATCTGGAGTTAAAGTTTCAAAATCAAAGTCATCTAACTTCATTGGATCTAATGGCTCTAACCCGTGGATGTTCATGCTGCTTAACTGATGATCAAACCCATCTAAATCAAGGTTTAACCCACTGTCTAGGTTTTGGTAGACAAACTCATCCAATTGTTCATTGGACATCTTGTCAAAGCTACTGGAGAAGTCCAAGAGATCTTCAATAATGGCTTGCGGTTCAGTCTCAAAGCTAGACAATGTTTCCATGCTGTTTAACAATAAAGGATCCTTTTCCTTCTTGTTAAAGCGCAACACACTGTCTAGCTTCGCCATCCCGTTTAAAACAGTATCACTGCAGAGCTTATAAACGGCTTTAAGTTCTTTTAGCGTAATCTGGATGTTTTTAATCTTGCTTTCGTTTTCCAAGATCGCTTTTTGAATGTTGCGCACTAACTCACCGGACTTTTGCAATTGTTCGTAACTGTCACTTGGACGGTGAGCAATCGTAAAGTCGTTGGATAAAGAGCGTAGCAGTTCATCCTTTTGGTACACCGAAAGGTCTTTAGCGTTTAAAAACTCCGCTTTGAGCTGTTCGTTTTCTTCAATTAAGCGGGTCAATTCGGCTTGTAATTCCTTTTTGATTAACAAGAAGCGGTTGGTAATGTCGGTTTTGGAATTAGTAATAAAGTCACTAATTTGGACAAACGACTTTTTGACCACCATTTGGTAACTTGACATTTTAAAGATGTCATCGAAGTTAATGGCAGGGTTAGAACGACCTTGCTTTAACTCGGCAATAATGCTGTTAATGTCGCTGTTGGCAAACAGATTAATTTCAGTAGTTGGCTGTAACTCCGTTGTTCTGAGCTGTTCTACCATCACTGGCATGAGTGGTTTGACTGGTTGTTGTACCACATCAACATTTGATGAAGCCTCTTTGGTTTCCACTGGTGGAGTGTCGACAAAGATTTCGATCGCAGGTGCACTTTCGACAACTGGGGTAGTTGGTGTTGAAATTGTTGGCTCTACTACCCGGTTGACCGTTTGTATGTTAATGCTAGATACAGCAGGTGGAACAGGAACTGTTTTCGGTTCGCTTACTCGTCATACAACCTCACTGTTACTGTGACAGTGGGTCTTGCTTTCCATACCCACTTCTGGTGGGGTAAAATCATCAAAGCTGTGGGTACCGACAGCACCGTGTTGATTGTGCTTAAAGTCCACAACCTCAACTAATGGTAAGTCAGATGAAGTTGTATTATTGAGTTCAATTTCAAACAGTGGTGGGGCAATTGTTGGTTCAACTGGACTGGTTGTAACGGTAGGAGTTGATTCCGCCACTTCAATTTTAATTGGGGTAGTTGGTTGTACTGGTGCAGTCTGTGGTTGGTTTTTTACTGTATCGGTGAGATGTAAGACATAATCCATATCAGCAACGGGTGCTACTGCTGGTTGAGGTTCTGCGGTAGCGGTAGCTTGGCTTTCTACTACTGTTACTGGAATTACTTCTGGTTGTTCAACAGGAGCAGCTTCTGGAGTTAGATTCCATTCATAAACCGCTGGTTGAGGTGCAGCTTGTGGTTGTGGTTCAACTACTGGGGTTGGTTCAGCCACAGCTTCTTGAACAGTTTCAACAACTGGTTCTGGTTGAACTTCTACAGGGGTTTCAGGTTGTGGTTCAACTACTTGTTCCACTTCTACAACAGGATTTTCAGGTTGAACTTCTAAAACTGAAGCTACTTCCGGGGTTGTAATTACTTCTGGTTGGACTTCCACAGGTTGTTCTGGTTGTGGTTCAACAATCGGTTCTACAGCTACTTCTGGTTGAACCATTTCAGATTGAACTTCTGCAACTGGAGTCGATTCCACAACCACTTCTGGTTGAACTTGTTCCGTTGGAATTAATTCAGACACACCTGCCACAGCTGGTTCTACTGGAAGTGGCTCGGCAACAGCTTCCGGTTGATGTTCTACAACAGCTACTACAGGCTCTGTAACTACCTGAGGTGTAATCTCTGCAATAGTTTCAACAACTGGTGTTGGTTCAGTTTGTTGTGCTTCTGTAACTGGCTCTGCAGCTGCTTCTGGTTGAACCATTTCAGATTGAACTTCTGTAACTGGTTCTGGTTGAAGCTCTACTGCTGATTGTTCAGCTGGAATCTCTGGTTGGGTTTGTACAGCTTGTGGTTCAACTTGTTCCAATATTGGTGCAGTTTCTCCAATTGGATCTAGACTTACTTCTAATTGTGGCTCTACAGCTACTTCTGGTTGAACCATTTCAGCTTGAACTTCCACAGGTTGTTCTAGTTGTGATTCAACAATTGGTTCTACAACTACTTCTGGTTGAATTTCTACTGCTTGTGGTTCTACTGTTGCAGTAGTTTCTCCCACCGGTTCTACAGCTACTTCTGGTTGAACATCGTAAACAGGCTCAGCAGCTACCTCTGGTTGTACCTCATAGCTAGGTTCAGCAACTTCTGGTGCTGGTACATCTGCTTCCAAACCAAAGTCTTCTGAAGCTGTAGGTGTGGTTTCAATGTTATCCTCAAAGCCAAACTGTTTCTCTTCCGCTTCGGTTTGGGGTAAAGTCGAGATCCATTGATCACCCTCAAAGTAACCAGATCAAACCCACTCCCCAGCTTCATCATAGTAACCATAAGCCTCATTACCGACATACTGAAGGTAATCCGGTTCTGCTTCGGTTTGTTCCTGTTCCCCCGCTAAAACTTCCACTGTTGGTGCATCAGCAAACACGAACGGATTACCATTGGCGTCAAACAACACGCTAATGTCGTATTCCGTGTCACCATAAGGATCATAGTAAACTTCAGTTTCACTGTTAAAGGCAATGTAATAACCATTGCCATCACCGTCATAGGCAATCTTGACTTTGCCATCTTGAACAGTTAATTTACCATCGTACAAATTACCAAAGATCTGTTCGGGATTTTCCTCAGTGTAATCCTTGTCTTCAAAAACTGCTTCTTTGCTTTTCTTCATGAAAAAAGAGCTTACAAAAGTGTAAGCATGGTTAACTTTGATTATAAAACTTTCTTTAAGGCCTTATCTTTTCCATGAGAGGTTGTATACCCTTAAATAATTTCTTAAATTATTTTCAGCAATCCTAATTAAGGGCTTAAACAAGGCCAAGTACAAGGGCATTAACAACGCTTGTACCAAGACCCTTGGCATTAAAAAGAAATAGCTACCATACTTAACGAAGTTCTTACTTGGTTTACCACTTAAAAAGACGAGGTATTCAATCGAGGCTATAGTGCCAATTAACAATGATACCACTAAGGTCATTAGTAAGGCCATCATTAAGATGTAGAGGTTTAAGATCATCTCTTTCGGTTTTTTGACCTTAGTGTACTTTCAACCCAAAAAGATTTCTAACCCCACAAAAAAGCTAATAAAGGCTGCCATCGTAATGCCTTGTAGGATGTGGACATTGACATCAAAGCCCCGGCCAAAGCTCTGGTTACCAGCAGCTTGAAAATTGCCATCAGTCAGGTACATTAATAATAAGACTAGGGTCAAGACAAAGAAACCAATCAGAATGCTTTGTAAGATCCAAAAGTCAATTTTTCAGTTAGTACTACTTTGGCGTACCTGATAAACGCCCTTAACCAGCCCCGCTAAAAAGCAGAACATGGGTTTTTGAATCGCAAACAACCAAAACCAGGTATAGCCCCTAGTAAGCCAGTCCATCGTGTCGGCAATGGCGCCAAAGGTAAAGCCCCACACCGGTCCAAACATCCAACCAATTAAGGCAAAGGGAATTCTTGTGAAACTCACCTTGAGCACACTAGTGACATTAATGGAAAAAATATTAAAGATAAAGCTTAGTGCCATTAACACCGCCGCTCATACTAACAATTGTAAGTTCCGCGTTACCTTTAGGGGGTAATACGGAAACAAAAAGATAAAGAAGGCGTGGTACTTAGTTTTTAATAGTTTGAAGGTCTAGATTACCCAAAAACAGCTGGGGGTGTTAAATCATTGTCGTTGTTAGGATCACAATCCGTTTTAAATGGCTGTTGCATTTCCTCTTGCACGTAACGGTTCAACACTTCGTTTGTTTGATCCGAACTTGGTTTAGGACTCAAAACAAAGGCAATAATACTAATGAGGGCAAAGGGAATAATGCTCGCTAGGTAAAATATGGAAAAACCAACAAAGATAGTTTTAAAGGTCTCACTGTTAATTTTTAATTCATTAGACCTTAAGGCATTTAACAGAAAAGAGGGGTTTAAAATACCACCTGTAATAATGTTACCCCCAGGTAGGGCATTGAGTGCCACAAACAAGAAGATTGTCCAGAAGATCCACTTAATTCTGACCTTATACCGGTAGTTGTCATTGATCCTGGGAAAATCAGTTACCCCAAACTGGTGCTTGAGAAAGTGCATTGCCTTAAAGGTTTGACTTCTAGTAGCAAAACTGTAGGGCTTACCTTGAAAGGCTAAAAGGAAGGAAGTACTACCAAAACCCAAGAAGATCAAGGTAATTAAAGTAGAAGCACTAACAGGGAAAAAGAGGTTAATGCCACCGTTTAAATCAACTAACGCTGGCCTGTCGGTATTATTCTGACCAGTAACCGCTTTAGTTAGGTTACTGTCAGGGTTTACATACCTGTAAAACAAGAGGTTTACCACCACGAGGTAAGCAATGGCGGCCGCTCCAATTAACAACATGGAGATAAAGATTGCTAACTGGGCTCACTTAATTCACTGGCTTTTAAACTGGAATTCAAAGATTTTGCCTACCATCCGTTCCGAGGTGACCCGCATAAAGGCACGTTGGCGTGCACGTGCTTCGAGTGATTCGTTGACAAAGTTTTCCAACTCTGGGCTTACAGTACCAGTTGTATGTATCCGTGGTTGGTTTAAATCAATTGTAACCGTCGGTTTATTAGCTCCATTGGTTGTTTTTTCAACAGCTGTTGGTTGAACGCTACTTAACTTACTAAAGCTATTTAAATCACGGTTAAAGAGATTGCACACGGCAATTGGTAAATAGTTAGCCAAGTCCGTGGCATCAGTTAAATCAAGTTTGAGGTTAAGTTCGGTGTTCGCTAGTTTGGTTTGCCCTAAACTAACAAAGCGCGCTTCTAAGTTTTGAAAAACAGGTTGAAAGTCCTTTTTCTTGGTTTTAGCCTTAGCTTGCTTTTGCTCTAAAAAGTACAACAGCTTTAACTGTTCGCGAATTGTTTTAGCAAACGCCGGGTCGCTTTGGATCCGCTCTTTGAGGGCATTGGCATTAAAAACAATGTCTAAATTGTCTTGTAACTTGGTTAAAAGATCGCTAAAAGTCATCTTGGTATAGTTTACTTTTGCGCAAAGAATCGCAACACGAAGTGCTTTAGCAAATGGATTGCTAAGCCATCATTGCGCTTAATTTTAATATCCAGCTGCCAAATCGCATTAATTAAGGCCGTTAATTGGTTGGTGGATCAAAAGCGGTACTGGCTTTGAATCGCCTTTAAACGAAAGGGATTGACATTTCACTGTAGTTGGAAAACAGCTTGTGGTTTAACGCCTTTGACCATCAAGGCCAAAAGTAGTTCATTTGCTAAAAATTCTAGGAATTGCGGTGGTGTGAGCTGCTTTGGTAAAAACAAGCGTTCAATCAACTTTAAGGCTTTAGCTGTTTGCAAACGCACCATGGCGTCAGTTAATTGTAAGATTTGACTGGTTTGGTAATCACACAAAACGGCTTCTAACATTTTTTTGTCCTGTAATTCTGCCGGGCTGAGTAGAGTTAACTTACGCAACTCCTGTTCAATGACCCCTAAGTTAAAGGGTAGAATCGTGGCTAAGTGGTCAATTAAATCAGGGTTTAAGTTGAGTTTGAGTGTCTTAGCGGCCTTACTGATCACCTGGTGGATGGTTTTAGCTGTGACTGGTTTACAAGCATACTTGGTAATTGCCCGGTCATTAATGAGGGCATTAGCGGGACTGTCGCTGTAAATGGTGACCACCACATCAGTATCTTTAAGTGTTTTTAAGCAGTGTAATTCAGCCTTAGTTAAGTTGTTCTTTTCCAAAAAACTGCAGTTGTGAATGAGGATCGGTTTAGTGTCCTCAGCAAACAGATTCTGGTTTAACACCACATCATACAGCTTATTAACATCTTGAAACCAGACCTGTTTACAAGCGGCTGGGTTGAGCAGTTGGTTTAACTGCTGGTGAATTAACCCAATATCGGCGCCATAAACTACGGTCATAGTAAAAGACTGTACCCACAGTATACAACAAGCCCCACAGCCCATAATAAACCAGGTAAAACACCCAGTTAGGTTTGGGTAGATTTAAAAATACGGTCGTGTTTAAGGCTCACTGACTCAACCAGGGAAGTGGTCAGTAAAAACCGACCAAACTGTTGCCCATTCATGGAATTAGCGGTAACAACAGTCAGCTAGCACAGAAGTAAAACAAGGCTATGGGAGTAAACAAGAGGTTATGAAACACACTTAAAGCGTTTAATTTTCGATTTAAGTGCAAAGTAATCGGACTGACCACAATGAGGATTAAGGTGGAAGTTAACACCGCTTGCAGTGGCTTTCACAGCTTCACCTTATTAATGGCAAACAACACAAAGCACGCTAAAAAGGAAAAGTTAAAGCCAAAGTTGTGTAACGCTCCCGGACTCATTAAGATGATTAACAGCGCCGTTAAGCCCAAATTATTTTCGGGTAGTTGCTTGGGAAACATTTGCCGTAACAGCTTTGAGACAAAAACCCGTAAAGCTGAAAAGGCAAATCCGACCAAAAAGAGGTACAGCAACAGCATTACAAAACCCGTTATTTTATTGATGTAAAAGCGCTTGTACAACCAGCGCTCCATGACACCAAACAAAAAACTAAGGTGAAAACCACTAATCACAAACAGGTGGACAATATTGAGCTGAATGGCACTCTGATACAGTGTGTTTTTTGTTGTTTCGTTAATTAAAAACAGCTTTAAGTACTGATTAAAAACACCACTAGTGTGTTGATCTAGTCAACTAATAAAAGGGGTGCGAAGTCAGTTCGCTTTCATTAGTTTGGCTAGTTGAAACTCTGGCATTAACTGACAGTATGCTAGTAAAGCGGCACTACAACTGATTAACAGTAAGCCAAACTTCCAGTGTACCGTTAAACTAATTAGGTTAACTATCACCAAAAAACTCGCTAAAATTACCCACTGATTTTGTTCGATAAAGAAGTAAGCAGGGGTAAAGGTGAGTAACGTAAAAAAATAAAACAGCTTTCACTTCATGCTGTTTTATTAATTGGTTAATTAGTTTAGAAATGCCAATTAAAACTCTTTACTCCAGCTGGAACGGAGCGGAAAGCGTTGAGACAAACCACCACGTAACGGTGAGCGGTAACGCGGGGTGAAACTGGTGTAATTGCTCCCTAAATCAAAGCGGTGCGTGTTGAAAAAGGAACTGCGGTTACGCCTTAAGCTCGACAATTCCAATGGTCTTCTAAGTGGGTAAAAGCTACTTGGACTGCGGTAAGCGCCAAAGCTGTTAAGGCTGTCGAAGTTCAAACTCGCTGGGTTGCGGTAGTTTACAGCCATCGGTGTAACACCAGTAAACCGACTGGCAATTGGTGTTGACTCTACTGTTGGCCGTGTAAAACGTGCTCAACTGTTGCGGATTGCTGGAAGGTTAAACAGCGGTTCGTTGTACTTGGTAGCCACATATTCACTCGGTGGAGCTAACTGGTAGCGCTCGTGCGGTAAAGGCGTACGGGTAGAAGCATCGTGGAGGTGACCCAAACTCGCTAGCTTTTCCAACAATAAGGAACGGTTGGTGGTGTGAATGGTAATTGCACCACTTTCATTATTAACACAAGTTGACTGACACGGTTGTGGTTCCACTACCGATGCAGGGGATGGTTTTCATTCCGGTTCGGGTTGTGTTGGACGGATAATTGGTTGGACTACCACTTCTGGTTGAACTACAACTGGTGGAACCGCAGGAGTTGTTTGGACTACCTTAGGTGTCACTTTAGGTGTAGCTACTTGAGGAGTAACTACTACCTGTGGTGCTGGCGTTGGTGTTACTACCTTCGGTTCTGCCTTAACAACTGGTTGAACTACAGCAGTTGGTTGGACAACTACTGGAGCAGGGGTTGGTTGTGGTGCTACTTTAGGTACAGCTGGAACAGGGGTTGGTTGGGCCACTTGGAGTGCAACTTTTGCTGTTACTACCTTCGGTATTTCAACTTTAGGTGAGTCAACCTCAACAGTTACAGGTGTTGGTTGTGGTTGTTCCACTACCTTAGGCTCTACTTTAGGGGCAGGAGTAGCTTCTACCACTGGTGTTGGGGTAGGTTCCACTACCTTAGGAGCTTCCACTACTGGTGCGGTCTCTACCACTGGTGTTGGGGTAGGTTCTACTGTTGGAGTAACCTCTACTATTGGAGCAGATTCAACAATAGGAACAGCAGTTGGCTCCACTACTGGTGCAACTGGTTCTGGTTGGAGTGGTGCTACCTCTATTGGTGCTACAGCACTAGCTTGATCAGGATCGCTGTAAGCTTGGGAATCATAGTAAGCGTTCGGATCGTGAGCTTGATCATAGTTCTGTGTATTGTAAGCATTAGCATCATAGGCTTGATCATACGCTTGTTGATCAAAGGACTGATCGTAAGCTTGCTGGTCATAGTAAGCGTTCGGATCATAGTTTTGGTCATACGCTTGCTGGTCGTATGTTGCTTGATCAAAGGTCTGATCATAAGCTGGTGGTGCTGTAGTATCCTGATATTCGTCTGGTGCTTGAGGTAGTGGATCGAGTCCGAACAGTTGATCAGCTTGCTGTTCGGGTGTAGCATCGGGATCCACAACTGGTAAACCAGCATCAATGTCCGGATAGCCCTCACCACTCAATGGTTCGCTTGTAGTGGTATCTGCACTTGCGGTACTACCACCATAGTTTAAATAGTCCACTAGGGGAATGTTTTCCCCATCACGGTAACCACTCACATCCAAAACATTGCCATCTTGGTCGTAAAAGGTATTGTTGACCTGGTCGTATTGGACTATTACCTCATTGTTGTTGCCATCAAAGGCAGTGAGTGGAATAATCTTGGGGGCAAAGCTAATTGGTTCATCACTGTCCAATTCGAACAGAGGATAATCCGCTGGGAGCTGTTTTCTTTTTTCCAAAGAAAAGATGTTCCCGGTTTCAGGATCCTGGTACAGGGCGTGAAGCTTATTTTTAGCATCACGACCATAAACCACATTAATGTCGGGATAGCTCTTTTGGATCTTTTGTGCTAGCTTACCATACGCTTTCGCTAGCTTTGCTCTTTCTTTATCTGTCATCTTGAAGCACTAATTAGCGTTTTGGTGGAAAACCGGGTTGGGGTGGCATGCCAGGACGTGGTGGTTGCATTCCGGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGGTCTTGGAGCCATACCGGGGTGTGGCGGCATACCTGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGGTCTTGGAGCCATACCGGGGTGTGGCGGCATACCTGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGATCTTGGAGCCATACCGGGGTGTGGCGGCATACCTGGACGAGGCGCCATACCGGGTTGAGGTGGGAAACCAGTACGGGGACCGAAGTTAATTTGGACTTGGGGTTGAGGTGGTACTGGTACTGGTTGTGGTGCTGGTTCAACTTCCGCTTCAGCATGGGCTTCAGCAGCTGCTTGTTGTTCTAACGCTTGTTGCTCTTCTTGTTGGGCAGAAATGCGTTGTAGCTGTTCCGCTAACTGTTCTTGGCGTTCCTTTTCCTCCAATAAGCGTTTTTCCTTGCGCTTCACAATTGGCAGTCCTATAGCAAGGCCCAAGATGATGGCTAGGGCACTAAAACCAAAGCAACCAGCAACTGTAGGAATAAACCATGCTTGCTCAGTAATCGGCTTGCCCTGAATTTGTACTGTATCCTCTTCTGCGTGTAAAACAACGTTAAGGGGACTCAATTCACTCTTAACTTCTGTCAGTTCGGTATTGTTGTGCTGTACCAAAATTAAGGTTGCAAGCACTATTAAAGCGCTGAACAGCACTAGCATTCAGGCTAATAAAAACAGTTTAAGCTTTCTTCGAGGTGGTAACTTCATGCACTAATTAAAGGGAGACACTAACGACGGCGGCGACGGGGTGCTTCATCATAGTAGTAATCATCATCACGACTTTGTTGTGCCCGTTTTGCTTTACGGCGCTTTTTGATGATGGCACGGGTAATCTTGGACAGGATAAAGTTAATAATTAAGGCACCTATAAGCCCTGAAACTAAGGCCATGCCCAACGGAATAATGTACTTAGTAAGGTAGGGACCAACCTTTTCTAAAAAGGCGGGGATATTGGGTTTCTTTAAAAAGTCGACATACCCTGGGATTTGGCTAAACAAACTACCATCAATCGGAAAGCGGGGATCTAGCTTGTTAGCATTATTAGCTACTTGTTCTGCTGCTAGTATCAAGGCGTGGGACTGTGATGCTGCAGCTGCTTTAGGTGCAGTTACCGTAGCGCCTACACCACTAGTAGTAACCGTGTCAAGCGCTTCCTTAGAGAAAAAACGGTTAAACACATCCATTGGTTGTGCTAGTTTAAAGGATGGGCTAACAGTACCAAAGTAAAAGATTGCTAAAAAGAGGTAAGCAATTGCTACCGTCAGTACCGCTAAGAAGGCTAGCTTAAAGAGCAATTTGAAGAAACCCATTGCTAGAGAAGAGTTAAATATTGGGAAAATTTTAACTTTTCAAAGCTTTAATTCCCCACTTACAGCTTAACTTCCTTAATTAAGAGCTTTAAAAAGCTCTGCTTAAATTACGCGTTAACAAAATAATGCGCAGGATAAAGTAGACCAAGTTCATGAAGCTACTTACCAACCAAGCGCCAAAAAAGAGCGCACTACGCCACATTTGTGCTTTGGCTAAAGCTGGGTCTTGACTTTCATACAGTTTCACCAAGTCCTGTTCGTTGCGAATTTGTAGACAAACAAAGTAAACCGATAACAAGGAAAAGATCCCACCAACTATTAACGAAAGTCCGGGGAACAGTAAATCCAAGATATTAGTGCGACCACCGCGGAACACCGTAAAGTTCAACACAAAAGTCAATAAGCTCGCAATTATGCTAATAAAGAAGGCTCAAATAAAGATACGAAACAGCGTTTGGGTTTGTTGGTAACGGCGTTGCTGGCTCGGTAATGCACTCAAGCCCCATACCGTCCCATACACTAATGCTGCCCCTAAAAAGGCAAAGACAATGTCACTAAACTTAAAGTCAATCAATTCACGTAATTGTGTGGTGGCTAATGTTAACAATACTCCTAATGTAATCCCATAACTAATCACATCGGCAATAATTAAGCCCCAGAACCACGCTAAGGTCGTGTTACGGTTCCTTAGTTTAAAAGCCGTGACAAAGTAAAGGATTAAGGAAACCAAACTAGCAACAAAAGCAATCGTACCCAACGTTCTTATATCACTAGCCAGTGTGCCAAGGCTAGTAGTGTTGGTTAACAGCTGTTGTACCCCAAAACCAATTAAGAAGATAAACAGAATGCCAATCGCGGCAACAGAAAAGGTCAAGCGAATGACCGAGAACCGCTTGTGATGGACAAACTGTTGCTCGGAAAAATTGACCTGATTATTAGCTTGATTGATCATGATAAGTCAACTGGGTCAATTTTAATTTGTAAGCGCTTAATTGCTAATAAATGCAAAATTATAGAAAGGGGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAACACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCACGGAGACTTACCCAAGTGGTTTAAGGGGGCGCTCTCGAAAAGCGTTAGGTGTTTTTGCATGCGTGGGTTCGAATCCCACAGTCTCCGCCAAAATATTAACTGTATCAATTAAAAAAGCACACCTTTTGGGGATGTGCTTTTTTTAGTGTTTCCTAATACTGACAACTATTTATTTTCGGGAATAAAACCATAGTCACGGATGGTACCGTTACCCGATGGCGCACTGCGGAATTGGAACCATGGTTTGAGCAGCGTTGGTGATGTTTCGTTCAACCCAGCTGCTGGTACGGGTAAAACCGGGAAGTAAGAGTCGACTACGGTAATTAAGAACTTAGCGTTATCGGTGTCTTTTTCCGCTCAAATACTGTTAAAGCGGTTGAGCTGGGCGGCGTTCTTATAGATATCTTTCAACTCGTAACCAATGAAATCTTCGTTTTTACTTGGTTTATTTGTACCGTTCTCTATCTTGTCACGCTTTCCAAGGTACTTATTTGGATCTTTGATAGCTTCCTTTACTTTTTCTTTGTCTAGAATGCCATTATCCACTAAGAATTTGAGCAAGCTTAAAGCCTTTTTGGTGAACTCTAAAGATGCTTGAGTGCCGTTACCACTAGTTGTAGAACCGGTGCTTCTTTGTGCAGCTTGTGCTTTAGCATCACCAAAGTTCTTTAGTACGTAAGGGTCATCTTTGAAGAGGTCAGCGGAAGTTACGTCATCCCACTTTTCTTCTTCAGTATTACTCTTGCTGCTATCTTGAGATGAATCTGCTTTCTTAACAGTCACATCAGATGTGGTTGAAGCTGTATTTTGTTGATCTATTTTTTGTGTGAATTTACTATGTACATCTTTTTGACTCGCAGCTAACAACAACGTACGTACAATGTGAGCGCCCGAGGAGTAGGTAATAACATCACCGCCACCTTGATCGTTCAATTGGGTGGCTGCGTCCAATCAGGTACCCACACCATCATAGTCAGGGCTTCAGGAACTAAAACCTAACACCGAGGACAACCCAATGTACCACTCATTTCGAGTTGGTCCTTGGGCATCACCTAAGATAATTTCTGGTACTATTAGTTTTTCTCCGTTGTCAGCAGTACCCAATTCAGTGAGGGCTTGCTTCACGGCGTTGTAGTAATCCACCCCGTTAGCGGACAGCGAAGAGTTCCCTAACTTCATGTTGAAGTGGAGGGGATTGCTACCAGAGAAACCAACCTTCTTTAAAGCTTGAACAAAGCTGTGCTTTGTTACGTTTACACTCTTAGCTTCTACTGAAGAGGTTTGGTTTTGTTGTGTGTTGTCTGATGTGTTGTTGTTCTTAGCAAACGCTGCAGAGGCAGCTGCTCCAGCCTGACCACTTGTTGCTTTACTGCTATAATAATTAATCTTAACAGTCGCACTCTCCAAAACGTTTTTATTCTTTTCGTAGTTACCTTCACGCTGTGGAATCAAGCCCAGGCGCTGTTCCTTCTCGATCTTATAGTAGTAATCGTTCATCAGATCTGAACCTTCGGTAGTTGAGACAGCTTTTTGTTGAACACTTGCACCATTTTTACCCTTTTCCTTAAAGATGCCAAAAGGTACGATCGAATAGTTCAGGTCACCGGAGTTTGGCAACAGGATAATCGCTAGGTTTTGTCAGTTAATTAAACCGTTAATGCCAGCGCGAATCGTTAACGCTTCTTCTGACTCACTGTTGTACAAGAATTTAGCCTCGGTTTCGGTAATGTTGGAGTTAGTTTTAACGGTTTCACCTTCCACTACGTAAGGTCGTGATGAATAGGTAATCTGGGCACTTTGCGCGGTCTTTTGCACTTTAACCCAGCGTAAGTCCCCCGTACCCGCAAATCGTGACCTAGCATCCTCTTGTTGGGAGTATGGCACCTCCGCAGAAGATAATCCTCCAGCGATATAGTTGGAGTAAAACACTTCAGGGGTAGCGCCTGGTTGGAAGTAGTTAATCATCGTTGGGATGGGTTTTTCGTTTTCTTCCCGCGTTTTTGGTAAGTTGGGAGTTATATGAGTATCATACTCACTGTTACGCTTAAAGACAATCTGGCTCTGGTTAAAACTCTCGACATAGTAAGGCCCCACTGATCAAGTATCCCGTCATGCGTTAACCCCACCACCATAGATACCATCAAAGTTAGTTTTGGTTTGGTCTAAGATTTTCGACCCATTCTTTTCGTTGTAGACCAATGGTGAGTCCTTACCTACCTTAATTGCTTTTACCTTCGGGTGGGTATGAGGAATAGGAAAGAAGAATTCCTTAGACATCATACTCAACAAGAAGGGGAAGGGCGAGGTGAGGTAGACGTTGAATTTGCTGTCATCAGTGCTTTGATAATCCTCAGGATTGTAAGCATCATCCGTTATCTTAACAGTTTTTTCTTCACCATCTTTTTCATCAGATGAAGCAACCTTCATTTGAACTTGGGTACCATTCTTCTTGGTCATCCCCACGGTCTTTTTCACATCCAAACCCATTAAGTCAATGAAGTAACCGTTGCGGTTAAACCTGAGTTCCGATGATAAGATGTAAGCCTCAAAACCGCGTTCAAAGTCTTTGGAAGAGAGTTTGACATCATTGCCACTAGCGTCCTTTACCGGTTTACCGTTATTGTCGACCCATTTGATACCAGTATCAATGGCGAATTCGTAACGGGTGGCCTTTTGCGAAATTTCTTGGAATTTTTCTTCCTCAGTAATCGACTTCGATTGTTGATTATTTTGACCGTTACTTGAACTTTGTCTTTTAATTTCACCAATGCATTCTCAATCATCTTCACCACTAGTGCTAGTACCACTAGAAGTGGTAGTGCAAGAAGAATTAGTTGAAGTTTTTTGACTTGAAGTAGCTTTATCCTTCTTCTTATACCCCTTCACTGAGGTAGCCAATTCCAACACCAATTTCTCTTGGGTTTGAGTGGTTACATTGACACCATCATCTTTGTTGGAACCAGTGGCAATGTAAGTGGTTAGGCCTGCATACTTGCTGCCAAAAAAGGAAGAGGTCGGGGACACGTTGAACATACTCATCGGTACCGAATTGTTCTTTAACAAGTCGGATGCGGATGAGGTGACATACACATCGCGGGCTTGGGCAGCGCAGGCACTGAGTGCTACTGCTGAACTTACCCCTAAGGTTGATAACAAACCCCAGTATTTTCGCTGAAATTTCATGGATCGTTCATAAATGTTGTTGATGATGTTTATTGCTATATGAAAAACATGGCATGAAAGCCATGTTGAACCATGAGCAACATACTCTTAGCTGTGAGCTACTAAAAGTGATTTTATTCTAATTTGGGAGCTTGCTAATCTGCGAAATTAAAGCTCAAGTTTCAAAAATTGCTGTTGCAATAAATTAATTCTTAATTTAAAGGCTTCAGCAAACGCTAAAACTTGGTGCACTCGAAGGGACTTGAACCCCCACTCCGCAATGAAACTAGATCCTAAGTCTAGCGCGTCTACCATTCCGCCACGAGTGCATATTGGTGCATCTTGAGGGGCTCGAACCCCCGACCCAATGATTAAGAGTCATTTGCTCTACCAACTGAGCTAAAGATGCATTAAGGGAAGCTGGTGCCGACTATAGGATTCGAACCTACGACCTACTGATTACAAGTCAATTGCTCTACCAACTGAGCTAAGTCGGCCTGGTGGATTGTGAGGGGATCGAACCCCCGACCCTATGCTTGTAAGGCATATGCTCTCCCAGCTGAGCTAACAATCCAGGCTTTTGGTGCCGAATACAGGAATTGAACCTGTAGCCTATGCATTACGAGTGCATCGCTCTACCCTTGAGCTAATTCGGCTTGGTGACGCGTACGGGATTCAAACCCGTGAATGCACGCGTGAAAGGCGTGTGTGTTAAGTCTCTTCACCAACGCGCCAAAATTCTTGGCGGCCACAACAGGGATCGAACCTGTGACCAACCGGTTAACAGCCGGTTGCTCTACCGCTGAGCTATGTGGCCAATAATGCAAGTACAATTCTAGAATTTCTAGCCCACTTTAACATCTTCCCGCGGATAAGAAAAGCTGTTTCCTTGAGGGTGTTTTTTAACAAAAGTGAGTACCGAACTGCTCACCCCGACCTGCCCCATAATCATTAGTAAACCTAACGTTAAGTAGTTAAACAGGTTCCGTGTTGGCTCTAGTGCCATGATCTTCGTGGCCCCACTAGACAGTCCGACAGTTCCAAAAGCAGAAGTGGTTTCAAACAGGCTGTCGACAAAGCTAAGCGGTTGTTGCATACTTAACGGCAACAGAATGGCAATAAGAACCACGGACACTAAGCCAAAGACGGCTACCAAGAAGGCATTAATCACCGTTTGCCCGTCAATACTACGCTTAAAGACCTTGACCTCTTTTTGACCGCGGAACTTCGCTTTTAACGTCAAAAAGATGACCGCTAAAGTAGTAGTGCGAATCCCTCCGGCGGTAGAAGACGGACTGCCCCCGATAAACATCGCAATCACCATAATCCCCTTAATGATGTCAGACTCAGCACTTCAGGGAAACACACTAAAGCCAGCGGACCGGGACGAAATCACCCCAAACCACAGTTGCATCACCCGACTAGCCGCTGGTATGGGACCAAAGATTAAGCTTTGGAGTTCTTGATTGGATTTATTTTTCAGTCCCTCCACTAAGTAGGCCTTCTCGACATTGAGATGGGCAATCGTATTGGTCAAACTAGTTATAACAATAAACTCCACCATTAACAGCATTAAAAAACACCAAGCCGTGACAATCAGGTTAGTGAGTACCGCTAACTTCGTAAACAAACTAAACTGGTGTTTGTAATACTTACCATACAATCGCCGTTTCTTAATCGCTTCATAAGCATCAAATAACACCGGATAACCAATGCCACCAAAGATAATTTGGCTAATCGCTAACCACTGGATCACGATGCCCCAGTTAGTGCGATACGGGGCAAACGAATTAGCGCCCAACAGGTCAATGCCAGCATTATTAACCGCTGACAAGGAGTGGAAAAAACCATACTGAAAGGCTAAGTTAAGGTTATGAACTACGGGTAATCTTTTGGTGGAATCGACTATGAGGGCCTTCAATTGGTTGGACACCTTACCACTACTTTGAAAAACACTAGCAGACTCAAACGCGGGGATAAAATAAAACAAAATGGTGTACAGAAAGCCATACAGTAACTCCACCATAAAGAGGAATAACACCGACACCACAATCATTTCACTAGTGGTCCCTAACTTGGAACCACCCCGTTCTGATTGCAACATTAACTTCTCATAAAAGCTGTACTTGCCCTTCTTGTGTAACTTAAACAGTCTTCAAACAAGGAAAGCAATCACCACAAAACCAATGCCCCCTAACTGCAGTAAAATGGCCAACACCGTTTGCCCAAACACACTGTAGGTTTCACTCACTACCGTAGTAGAGAGCCCCGTATCACTAAAGGCACTGACCGCTAAAAACAAAGCATCCAAAAAGCCATAGGAAGCCGTTTGTTTACTGATGGTATGACCCTGTAAATCCACCTCATAGCTAATAACCTTTTGGTAGTCGGTTTTGAGCGCAAACGGGAGGAAAAGCAGTACAGCCCCCAACAGGATGCAATAGATGTAAAAGTGGAAAATGCGCTGGGAAATACTGTCACCCCACAGGATTAGTTTTAACCAAGCGAGTAGTTTAGCTTTCTGTTTATGAGCAGCTGTCATGTAAATGACAATATAAATATAATTTTACCAGCATATGAAACGTGCCGATTTTTGCATTATTGGCCTTGGTAGGTTTGGGATGCAAGTAGCACATTCTTTAAAAGAGAACAACTTTACCCTGGTTTTGATCGATAATGATCAACACAAAACCAATACTGCTGCCCAGGAATTTGACCATGTAGTGTGTTGTGATGGGAGTAACTTAACGGCGTTAGCTGAGCTCCAACTAGAGGAGTTTTCGGCAGTCATAGTTGGGGTAACTAACATAGAAGCGAGCATTATGATTTGTGCTAACCTTCGGGAATTGGGTCAAAAAAACATTATTGCCCGTGCTAAAAACGAAGTGCATAACCGGGTGTTACGTACTATGGGTATTAGGGAAGCTTTGATCCCGGAAAAGATTGTCGGCAAGAACCTTGTCATTCGTTTAATTCACGGCATGGAAACCGAAATTATTAACCTTGGCAATGACATCCTCTTTGTTCGCGCTCCGGTGAACAACAAGAAGCTCTTTAATCGTACGTTGGGGGAAATTAACATCCGTGAACACACGAATGCTAACCTCATCTCAATTATGCGGAATGGCAAAGATGTCGTCTTTCCTTTGGGTCCGAATACCCAGATTCAACCAGGGGATGTAATTACAGCCGTGTGTCAATTAAGTGGCGTGAACCAGTATTTGCGCTACATTAATCCTAACGACAAGAATAAGTACAAAGCGAGTGAATAATTAGATCACCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAAAGGGGATTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCAGACAGCACTAACCAAACAGGCGCAATGTTTGGCTTGAAGGTGAAGGATGCAACCGTGGATAGTTCGAAGCAATCAACGGAAAGCTTAAAGGGCGAAGAATCGAGTTCCAGTTCCACCACATCTTCCACCTCCACCACCCAACGTGGGGGTTCGTCAGGGGACACCAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCGGACTCGGAGGACAATGGTCAGATCGAACTTGAAACCAACAACCTCGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGAATCTGGTCAATCAGGCACCCCCACCCCCTGAACGCCGTGGCTTGCGACCGAGCAAATTCACAAGGACCTCCCCAAATGATCCGCCTCGATCCTGATTCTGTACGATGCGCCTTATGCGCGCAATCGTACCGCCATTGATCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGGTTGTGGGACTGAAAGGCGCGCGATGTCCTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCAGAGTGGTTTGACCAGGGCCAAGCGGTCGCGGATAATACCCAAACCGGGTTTGATACAGATGACACCGATAATAAAAAGCAGGGCTTTCGCAAACAAGGCGAACAATCCCCTGCCCCCATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGGGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAATTAATAAATAGGTCTATGGATTAATTACCTCACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTCGTGCGCGCCGCCCATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGGACAGAACAAGTTGGTGGCGCTGGTCCGCTCAGCGTTGGTCAGGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGTCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAACTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCGCCCAGGGCCACGTGGGTGTTTGAACGCAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACCTCCTCGTCGAACAACCCGTGACCCCTTACACCCCGAATGCGGGGTTAGTCCGGGTGAATGGGGTTGCTCAGGATACGGTTCATTTTGGTTCGGGTCAAGAATCGAGTTGGAATTCCCAACGTTCCCAAAAAGGCCTTAAAAACAACCCCGGACCCAAAGCCGTCACCGGCTTTAAGCTCGATAAGGGCCGCGCGTACCAGAAGTTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACGAAGGAGGGGAAGGAGAAAAACGAATCGGAGTGGAAGTCTTCCGGGGCGAGTGAGGCAAAAAATGATGCCCCGTTGGTGGGATCAACCGGGAGTCAAATGGCTGCTGTTACAGACAATCAGCAATCAGGTGATAACAATGGATTGGTATCGCTTGCACAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCACCCAGGCCCTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGCTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGACTTCTACAAATGAAGATTTGTCGAATGTAATAACACAGCTTTATCATACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATAGTTGTGATGGGCTCAGACCGGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCCCACACCGAGCTCAACTGGGGCACCGACAAGCAGAAGCAGTTTGTCGAAAACCAGTTGGGGTTTAAAGATGACTCAAATTCGGATTCCAAAAATTCGAATTTGAAGGCCCAAGGCCTCACCCAACCCGCCTACCTCATCGCCGGTCTTGACGTTGTGGCCGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGGGCAGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGTGCTCGCCTGGTCGACCACGGCCGGATTGGACAGTGATGGGGGGTACAAGGCCTTGGTGGAAAACACGGCCGGATTGAACGGACCGATTAATGGCTTGTTTACCCTGTTAGACACCTTTGCCTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTAAGAATACGGAGGCAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCCAAAATTGCTTCCTTAATTAATGCCAGTCCACTCAACAGTTATGGGGATGATGGCGTGACCGTGTTTGATGCCCTGGGCCTTAACTTTAACTTTAAGTTGGACGAGGCGCGCTTGCCATCGCGCACCGACCAACTGCTTGTGTATGGGATTGTAAACGAAAGTGAACTGAAGTCCGCGCGCGAAAATGCCCAGTCGACATCCGATGATAATTCAAACACCAAAGTCAAGTGAACCAACACCGCCTCGCACTACCTCCCCGTGCCGTATTACTACAGTGCCAATTTCCCCGAAGCGGGTAACAGGAGAAGAGCGGAGCAGCGGAATGGGGTGATAACAATAAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTGACGATTGGAAAACTAACAAAAGCCGTTAGTTTGATGTAGAGGCAAAACTATGAATTCACTAAATTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGCCTGCTCCCACTAAATAAATTGAGACAGATTTTCAAAGTAGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTTTAATACCTTCTAACTATTAATAGGAACTTTTCTTATTAATAAGAACTTTTTTGAAAAAAGTTTTGAAGTTGTTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAGCACAAAGACTCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCAGTGCTGCAAGTTTTAGTGACTTTTCAAACCTTGCAAAGAAAGGAGGTATTACTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGGTGAAAAAGACCAAAAAGGGCACTTTTTCAGGTTGATACATCATTAATCCAGAAAACAACAGTGTGGAATTCTATGGCAAATTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAGTTCAACGAGTACAAAAACTCAAACAACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGCGAGCAAATTAACAAACTGACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAGAGAACTTCAAGTAGAGCAAAAATTTCAGGGTGAACAAATAAAAGCTCAAGGAAAAACGCTTAAGTCGATCCTACAAGCGCTTGGAGGAATAAATAAACGCTTGGACAAAATTGATCCACCTAAATAGTTTTTGGGATAATAACAAAAGTGCCAGCAGTTAATCTTAAAGATTAATTGCTGGTATTTTCATTCATTTGAAATGAACAAAAAAGCCATCCTATCAAAGGTGAAAGATGGACGGCTAATGAATTGAAATCAATTACTTAATTAAATATTCCAAGTAAATAAAGAGATTAATATTTGATGAGTAGCCTTCTGTATTATTAGCAAAGGTACTTAAAAATTTGTTCACACTTTTTTGACTAACATCATCTTTGCCTTTTAACGAAAATTTAAGTGAAGCGCTAAACTCAATTCACTCTAAATTTCTTGTAGTTTCTTTCCCACCAATAGTTGTAGAGTTGTCTTCTTCAGTTTTAAAGTTTGCAATTGTAAATGATTTTTTGGCATCAGGTTTGATTTTAAGGAAAAGATCAAAATAATGCTTATCACCTTTCTTAGCAGTGAAACCACCTGAAGAATAATCTCCAAATCAATTAAAGGTATAGCTTAACTTATCCTCAACTGACAAATCCTTAAAACTTTGGTATGTCATCTGGAAATGTTCAATCTTTCGCTCCATCGTAAAAGGATCCTTGCTTGCGGGATAAATTACATCGTCAAAATCAAAATCTGGAATACCTAATTTACCCTCTTCAAAAATCTGTTTTCAAGCATCTAACAACAATTTTGTTGTATCTCTCGGATTAACTTCGTAAGACTTTTTTAAAGAAGCTATTATGTTCTTTTTGCCTTCTTTAGTTTTAATTGCTTTAGACAAATCAATGTCTTTACCAGTGGTTTCTTTAATAAGATTTCTAAGATCTGCTTGGATACCAGTGCAAGCACTTAACAATGTTCCACATAATAAAAATAGAGAACTAAAGGAAATCCTTGACAACAATCTCTTTTTGCGCATAAACAGTAAAAATAACTCTTTGATTTTGCTTTTTTTTGGAAAATCCTTTTGAATAAGAGGAAACTAATTGAAATTTCGGGTTTAACATTAGTTAAAAAAATGAAATTTCTAATTATTTATATAACAACTATCTACTGATAAACGTCTCGATCTGAGTAGTTCTAATAATTACCAATTGTTGGTATTTTTATTCATTTAATGCTTTCTAAAAACTTTTTTTTGCAATTTTGTTTGCACTTCTCAATAAGCCGGTCAATTTATTTAGTGGAGGAACAAAATGCAAATCTTTTATTTTTTGAATTGATCAGCAAAGCGTCTGATCCCTGTTGGCAACCGAGTTGCAAGTGTTAGTTATTTTTTAAAAAATCAAAAGAAAAAAGAAAGGAACATTTAAACATGAAAAAAGTTAAAGCATTAAGGAATCTTTTTCTAGAAAAAGCCAGTGAAAAACTTGAATACCCCAAAATTGCTTCAATGGTAGCTTACTTTGATCCAAAGGAAGATGATACTTGTGAATTCATTATGAAAATTAAAACTGTCAATGAAGTTGTTGACACTGAGACGACAAATAAAGAAAAGCCTTTAGGCACTTGAAAGAGTTAATTGATTTTTTGGTCTAAAAAGACATAAATTTACCAACGGTAAGTGTTAAAACTTGCTGTTGGTATTTTTATTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGGCACTCCTTGACCAGGTGTTGGATTATATTCCCTGGATTGGGAATGGGTACAGGTATGGGAATGGATATAAGGGGGTCGCTGGAGCGGGTCACAGCGCCACCAACGGCTCGCCGGCGGGCAACACAAGTTCCACCACGCAGTCCAATGATGTTGCACCCACTTTTAGCAATGTCGGCGTTGGTCTCAAAGCGAATGTCCAGGGCACGCTTGGTGACAGTCAGACGACGACTGTGGGTGGCCATCAATGACCCACCCTCGACCCAGCCAACCTCCAGCTCTGAACCGGCGCGGGGTGAAGGAATGATTCAAAGACGCAAAATAATACTACGACCAACGAAAACCACACCAAGTTCGCGAGTGCTACGGGCAGTGGTCAGCAGCAGGGTTCCACAACAACTACCTCCGCCGGCAACCCCGACTCGTTAAAGCAGGATAAGGCTGATAAAAGTGGTAATTCGATCAGTGTTCAAGAAGCCACATCGGGCGACAACTTGACCAACTACACCAACCTCCCCCCCAACCTCACCCCCACATCCGACTGACCGAACGCTTTGAGCTTCACCAACAAGAACAACGCCCAGCGCGCCCAGCTGTTACTCCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTGAATAATTAATTTAAGGTCAAATAAAGCAATAAAAAAAGGATCCAAACAGATCCTTTTTCAAATAGAACGCTGCCAATAAGCCATGTTCTGTTTTAAAGTCAGTTATTTATCTACAGTAATCCTGTCATTACTTTAATTATTTTTCTTAAAGTAACATTCCCTTACCAAAATTTAGGTTTCTTGCTTGTGGAGTTTACCGCGTTTCATACTTAGTTTTCACTAAGCTCGTCTCTGTGGTACTTTACGGACATCATCATAGTCAAAGACCTTAGACTAGTCCAGTCGTGACGTCTATTAGCGTCCTAAATCTTATTTGTTCAATTTACACAAACACTACTTGCGTTCCAGCAAGTGCAAGCATGGACTTTCCTCTACTCATTGAGCAGCAACTGACCGACAGCGGCTAAAATTATAATTTGAGCGTTGGGGATGTAGAGGTTTTGACATAATGTTGAAAGGAAAACAGTGGCAGTGGGGTATGCCCCTTACAGCTCTAGGTATAATAACCGACAAAAATAACGACGAAATTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAGAACATACTAAAGCTACACGAATTGAATCGCCATAGTTTGGTTCGTGTCACAGTTTATGGCTCGGGGTTAACTGGTTCAACTTAATCCTTAAATTATGAACTTATCGTTTACTTGTTTGTCTTATGATCTAAAGTAAGCGAGACATTAAAACATAAGACTAAACTGTAGAAGCTGTTTTACCAATCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCATTAACAACGCAAGTTGACCAAACCTTCCTTCGGGAAGGTTTTTTATTGCTTACGTTTGCGACAAAAAATGGCCTTAGCGCGTTGCTTGTTTCAGTTAAAGGCAAACAGTCCTAAAAAAGGCAATTCATACACCACAAGCGTAATACATAAAGCGGTGGCAAACGCTCCTAGTTGGTTTAAGCGCGAACTAAAAACGTTGTCTTTGTGAGAAATCCGAAAGAACAGTGACAGGTTAGGATTACTTTTTAGTTGCGTTGTCGTACGCGGGAAAAAGTGTTGGTCCGATAACACCAAAAAGAGCAACATCAACGCAATTCAAAAGCAATAAGCGACCACACTAAAGTAAAAACCAGTACTAAAAATGACCCGTTCTCACTTAGTGATTTGACTCTTAATAAACAACGAAATTGCTCAGCGGTAACCCAAAAAGATCATTAAAAACAAGCTAAAGAGGCCCAACGCTAGAATGGCAATACCAAAGCTGCTAATCGAATACACAAAGTTAGCGTTAAAGATGTCAGCTGGCTTTTTGACATTTCAAAACAAGTAAAAACCACTAATGCCCTGGTTTTGATCGTTTAAGGTAAACTGCTCGGTGTGAAAGATACCCACCAAGAACATCACTGCTGTCACAAAAAAAGACAGCAAGAACAAAAAGTAAGCAGCGGTATCTAACAGACCAAAACGGCTGTAGAAGAGGTACTGACTGTGTTTGGAAGCAACTTCGTATTCAACGGTATTAATTTCGGTGAGTTTATCGTTGATCTTACTCATCCAACCACCGCCGCTAGCAACGTTCTTTTTCTTGTTAGTCTTAGAAGGCACGCGGAATCTTGGCTGATAACCACACACTCTTGTGGTCTTTAACTAACACCATGTCCTCAATGCGGATGCCACCAACACCAGGGATATAAATACCCGGTTCAATCGTAATGACAGCATTTTCACACAACAACTTGTTATAGGACGTTGATACGTTTGGCATTTCGTGAATGTCAAGTCCAACACCATGACCAGTGCTGTGAACAAAGTAATCTTTGAACTCCGATGCTTCAATAATGTCACGGCACACCTTGTCAACTTCCGCACCGGTGAGTTGGGTATTAGCAGCGTTAATGCCGGCCATATTGGCTTCATCAACCTTTTTATAGGCCTTGAGCAACACTTCATTATTAGGTTTTTTACCAACTAAGAAGGTGCGGGTAATATCGGAACAGTAACCGTTGTAAATGGTGCCAAAGTCACAGGTTACAAAATCACCTGATTTTACCTTTAACTTGGAAGGCTTGTGGTGTGGATTAGCACCGTTCTTACCAGTAGCTACAATAGGGTCAAACGAGTTTTTTGCCCCACCAGCTTTGACCAATTGGTCAGTAATCCACTGCGCAATGAACAACTCCGTCATGCCTGGTTGGATAAAGCGCTTGAGTTTTACCGCTACTTTGCGGGTAATTTCCACTACCTTTTCAATCGCTAAGATTTCACTCGGGAGCTTTTGTCGGCGAATTTCCTGGGCGTTAATGACCGTGTACTGCGCACACAACTTCTTAATGAAATTTTGGTAGTTAAAAGTGAGGTAATCCCCTTCAATCAAGAGGTGTTTCACACCCACTTGTTCACATAATGCCTTTACTTGTTTATAAGTGGTAAATAATTCCACTTTGACTAGGGGATCAATTGCCGTTTTAGCGGCTTCAAAGTAACGCCCGTCAATAAAGAGGTTAACCCTTTGCTTGTGAACCACTAACCAACCAGCACTGGAGGGAAAACCAGTAAGCCAAAAGCGGTTTTGGTCTGAGCCAATTAAGATGGCATCAGCTTTATTGTCTTGGAGCAGTTTGTGTAAAACTGCTAGCTTTTGTTGCAGTTCGTTATGCATTATTTTCGTTTTACTTCCTTGTGTACTACTACCTTGCGACAACGCGAACAAAACTTGTTGAGGGCAAGTTTTTCAGGGTTTTTCTTGACGTTTTTAAACGTTAGATAGTTAATTTCACGACAATCATTACATCCTAGCCGTGTGCTTCTCTTAACAGCCATAAAGTATATAGATTCTAATTATTTATTTTTATCGAGTAATAAACTGATCGAAAACGCATCTATTCCGGTATGCGCAATAATGGCACTTGTGATAAACGAGCTTTCGATTTCACGTTGTAAAGTGATTTTGTTAGTGTGGAGAAAATCCAAAATTAAAGCTTTAACTTCCTTGGCCTTAACTGGGGTAAAAGCATTGCAAAAACCAATCCGTTTGATCTTAAAGTTATCACCAAATTTTGATTTGACAAAGGTAAAGATTTTCTCCAATGCTTGGACAAAGGTAAAGGCCTTGCCGCTCAAAGTATTTACCCCTTTATCAAAGAAGATAATCGGTTTAACCCGTAAAAGTGTGGCAATAATTTTCTTTAATCCAGAAATGCGCCCACCTTTACGTAGCTGCACTAAGTTTTTTACAGTCACAGCTGTAAAAATACTTTGTTTGTGCTGATCAACCACTTCAGCAATTGCTTTAGTTGGGCAGTTAGTGTCAGTTAATGCCTTCACCTCTTGTACTAACCATTTCAGGGAAATGGCAATGTCACTTGTTTCCAAGACCACAAACTCTTTTTTGGGCGTACTGAGTGGCTTAACCGCTTGGACAATCATGTCATACGTACCACTCAAACCCTTACTGAGAGGTAAAAAAACAAAGCGATCGTACTTGTCTTTAATGGCTTCAATTAACTCAATTAAGTCAGCTTGGCGTGGTAGTGATGTGGAAATGTTTAACCCTTGGGGGTGTTCCTTCAACAACTGGTATACTCGATCATAGTCAATGTCCTTACCATCGCGGTAAGTGTCTTGACCGTTAATAATGACCTGTAAAGGTAACACGTAAACGTCTTGGATCTCTCCTTCCTTAATTGAGGCAGTCGAGTCAGTAATTATCGCGGTCTTCATGCGCTCTATTTGTCCTTATTTCGCTTTATATTATCTAGAAACTCCCCAATGTAATCAAAAAAAAGTTTGGGTGCATAGTAGTGGGGTGAATGTCCTACACCATCAATAATTTTGAAAATGATCGTTTCGCTGTGTTTAGCTAAGTATTCCACAGAAGCCTTTGTGGGGGTGACAATATCATTGGAACCGAGAACCACTAAGGTGGGTTTGTCCTTAATTGCGTTGTAACCCGCTTCGAGATAATCATTACCATACTTGGCGTTTTGCACCATGTCACTGTAAAGTGTTTTAAAGTTGACTCGCTTCTTAAAAGCACCAACAAAGAAGTTAACAATCGACTTCTTATCATCGGTGTGTTCTATGAAGTCCTGCTGGTTTTTAGGACTCCTGGTAAACAAGGTATCCAAGATCCGTTTTTTACTTACAACAAACGATGTTTGATTCATCGGTGCTACCAACACTAAGGCCTTAATGCGTTCACGCAATACAGCATTGACCAACACCGCAATCGCCCCGCCCATACTGTGCCCTACCAATACCACCTTTTTCAAGCGCTTTTGGATGATAAAGTCACACACCAATTCAACATAGTGTTTCAACTTCAATTCATCAACACTGTTACTCTGATTATTGCCGTGTCCCGGAAAATTAAAGGCAAAGAACGACCAGCGCTTTTTTTCAAACAGTTTAAACACGTGTTTAAAGCTGGAATACTCACTGCCAAAACCATGGAGAAAGATAAAGTTATGGCGCTTACGGTGTGGTTTAAAGGCAAAGATTGATTGAAAGGGATCGCTCTTACTGCTTGCCATTGATCTTAGCTAGGCGTTCAGCGTACGCTTGGATCCGTTCCTTTTTGGATAACTTGCCCAAAGCATCTTCCTTGTTTTCGATCACAATATCACGTTTTTTTAGCCGTGGTTTCTTTTCGGGTAGCACTGGAATTTCCTTTTCCACAATAATGACCTCTTTAACAGTTTCGTCTTGCTTTGTTTTAATTTCGTGGGTTTGACTCGAGTTGAGTTCGTTGAAGTATTGCAACTCGTGTACTAAGGCTAAGTTTTGTTGCAGTAAGCGTTGTTTTTCTAGCTGCACTTGGTTAAATTGTTCTAACAATTCATCGCTTACCGGTGGAGTTGCTTGGAGGTTATTGTAAGCAGCTTGGAGTTGGGTTAGCTCTGCATGGGTTTGACTTAAACTTTGGTGTGTGTTGTGTAACTTTTGCGCTAACTGTTGTTTTTCCAAATTGACCTGGTTTAAAACTTGCAACTCTTGCTTGAGCTTGTTGTGATTACTTTGTAACAACTCGTAAGCGGAGAGCAAGTCCTGGTACTGGGCGTTGGCCTCATTAAGGTTAGTAATTTGAATTTCGTTTAATTCTGCTTTGACCTTGTTGTAACTCTCTTGCAGTAACTCATGCTCCGCTTGGATTTGGGCTAAATTACTGTGTTCATGTTGCATTTGCTCTTTTTGTTGAGCATATTTGTCTTGCAACAAGTTATGCTCTGTATTGAGGTTCTCCCAGTTAGTCTTTACCCGGTTTAAATCATGCAACTCATTTTGAATGTTACTGCATTTATCAGTTAATTCCTCGTTGTTTTGCTTAAAGAGTGCTAACTGTTGGTGGGCAGCATCAAGTTCATTTTGTGCTTGATGGAATTGTTGCTGGGTGTGATTAGCTTCATCTTGCAATTTGTTGTAACTCTCTTGCAACAGCTCGTGCTGCGTTTGGAGCTGGGTTAAGTTTTCATCACTCTGTTGCAAGTGGTTTAACTGGGTTTCGCTAGCCATTAGTTTGTCAGTTAGGCTTTGGTTTTGTTGTTTGAAGGTAGCTAACTGTTCTTGGGTTAAGCTAAGGTCAAACTGGGTTTGTTCTAGCTGTTGCTTTGTGAGTGTTAGCGTTTTGCTGTCAAACGCAGTTTTTTCCTTGTGGGTAAATTCTAAAACATTTAGTTGGTTTTGTAGTTCACTGTTAGTAATACCCAAGGCATGGACGTTTTCCTCAGCTGCTTGTAAGCTTGCAAGTAAACTGCTGTTTTTTTGTTCTAGTACCACTTTTTGCTGTTCAAAACCTTGTGCTGCGAGACCTAACTCAAAGTTAGTGGCCTTAACTTGGTCTAACTCTTTTACTAAAGCTTCGTTGTGGCGTTGTAATTGTGCTAACTGTTCCTGGTGGTGTTGGTGTGCTAGGTGTTGTTCGTGTTCCAATTCCTTTAACTTAGCATCACTGTCAGCACGCATTAACTCATACTGCTTTAAAAGATTACGGTACTTGGTGTTTTCATCGTTTAACTTAGTTTCACGTAGCTGTTTAATTTCGGTTTTGAGGAAGTTGTTTTCACGCTCAATTAGGCTTAAGCGTTGGTTAGTTTGCTTTAAGCTAATTACTTCCGTTTCCAAACTGTTAATTTCACTGCGTAACGACGGAATTAAGGCCAGTTGTTCCTGGTGTTTTTTGGAATCAAACTTGTACTGTTCGATTTGGAGTGTTAAGCTGTGTTTGTCTTGTTGGAGTTGTTCAAGCTGTTTTTCCAAGACAAAGTTTAACTGGTTAGCTTCCGGTAGGTTGTTTTGTAAATGCTGTTTAAGCGTACGGTTTTCCTCTTCCAAACGTGCTAAGAGTAAGCTGTTGTTTTGGCTCTTAAACAGCTTTTGTTGCAGTTCATTCAACTCCTGAGTGAGGTTAGTATTTTGGTTTTGCAACAGTTCAATCCGTTCCTGTAACACCGGACTTTGGTCGTACTGAAGTGGCTTTGCTAACTGCTCTTCTAGCTGGATTTGTAAAGCAGCGTTGTGCTTCAAGGCCTTATCTAACTGTTCTTGGAGCAGGGTCATTTCAAAACTCACGGTAGGTTGTACTGGCACTACATCTTGAACAGGCAGTAGCTTGTATTCTTCCACACCCATGTAAGTTACAGGAGGTGTTTCTAGTTGAGCTTGGCGTAGTTCAATTTGGGCTTGCAATTGTTGTAACTGCACTTGTTTGGTGGCAATTTCCTTGTCTAAAACGGCACTTTGATGAGCGCTGTCTTCAGCGGGTTTTATAACCACTGTTTGGCTCAAAGCATTAGTTAAATTAGCCTTGTCCACCGTTGTAATCGAGAAGCTATGTTCAGCTGCAGGTGATGTCACAACAACAGGTGTAACTGGTGCAGACTCGTTGACTAATGTATGGGTGAGTGGTACTGTTACTAATTCATCGACTAACTGGGTTTGGTCAAGTGCAAAGTTAGTTGGTGCTGCTTTTGGTTGTAACGGTGCTGGAGTTACAGTGTGAGTTAAAACTGGCACTTCCACTAGTTCGTCAAGCAGTTGTGTTTGGTCTAACGGAACAGTTGTGGTAGTTGGTGCAACTGTGTCAAATACATGAGGTACCTTCATTTCCGGGTACACCAAAATGGCATTACTGTCAATCGGACTGCTAAAGGTCAAGCTAACAGTAGGATCAAGTGCTGCTTGGGGTAAAGCAGTTTGGGCTTGAACATCCTGTTCCACCATTTCGACATAGTCATCCAACAACATAGTTTCACTAACAGCTTTTGTGGCTGCTGGTTCTGTTGCCACACTAAACTGATGGGTATGGTTTGATGAAACTTGTTCTGATGCGTTTTTGATTTGTTGGGTTAGTTCAATGTATTCGTTGACAAGCACACTTTCATCGACTTGTTGGGTTGAAGGAGTATTAAAGACCTCTTCAAACAGTACTTCCTTAGTGGCAAAGGATATAGGTATTGGTGTTGTTTCTAACTCTTTGGTGTGCACGTATTCATCGACTAGTTGAGTTTCATCTAACTGGGGCTTGGTCGTTTGTTCGGTAATACTAGAGGGGGTAATTTCACAAAAACCAGCCTTAATTTCCTTTGTTAGTACTTCCTGGCCTTCTTGTTCTAAAAACTCCATTGTTAGATCAACGGTTTGCGGTTTATGAACTTGGAGTTGGTTAGCGAAGTGGTTTAACACATCAACATGGGGTTGGCTTTTGACCACACCCATATCTACCATAAAGTGAAAAAAATTGCGAATTGATTGTTCTTTCTCTAACTGATCTAATTCGCCAAAGAAACTACGTTCTACAATGTTACGTTCTAGGGGAACTGTTATTGTTTCCCTTGTTGGTTCTAGGGTAATGTTACGGGTTTTGGGCATGAGTTGCTTGAGCAAACTTTGTTCGTTTAAGTTTTCGTCAAAATCGTGGATACCTAGCTCTTTTTCTGCTGTCATGAATGGCACAGACCTCATCTAGTTAAGTATATTATCTTAGGTTTTTGCGACACCTCTTATCCGTGCATTTTTGGACTTGAAATAAAGAGTAATAGGTACAGCACTAAAACCAAAGCTTTCCCTAATCTTGTTCTCTAAAAAGCGCGCATAAGAAAAGTGGACAAACTTAGGGTCATTACAGAACAGTACAAAGTGCGGTGTTTGACTGTGAGTTTGCACCGCATAAGTTACCTGTAACCTCCCACCCTTAAACAGGGGTGCTTGGTTAAACAGTTGTGCTTGTTGGATGACATCGTTTAACAGGGAAGTTGAAATCTTTTTGGTGAGCTGTTCCTGGATAATTTGGAGCTGTTCAAAGATGGTATGTAAGCGCTTGTTGTTTTTGACACTAATAAAAACAATGGGAGCAAAGCTGAGGTGTTGAAACTGACTTTGTAACTGCTTTTTAAACTTAGCCATCGTGTTGCTGTCCTTCTGTACTAAGTCTCATTTGTTGACCAGAATAATCACCGGAATTAAAGCGGCTTGGGCTAATCCACCGATAATCTCGTCCTGTTCACTCAACGGTTTGGAACCATCAACCATTAACAGAATAATGTTGCTCCTAGCGATTGCTAACTGGGTTTTCCCATACGAGGCCGCTTCAATTCCGGGGGCTATTTTCCCCTTACGGCGAATCCCAGCCGTATCAATTAAAAGGTAGTTTTGCCCATTAACCCTTAAGGGAATATCAATTGCATCACGGGTAGTACCACTCTCATCGGACACTAACACCCGGTTTTGCTGCACCAGTTGGTTAATTAAAGAACTCTTGCCAACATTGGGTTTACCAATTACACAAAAGCGCACTTCGGGATTGGTTTCACTACTACCATGGTAAGGGGGGAGTTGGTTGTCCTTAACTAGTCGATCCATTAGGTTACCAATGCCAATGCCATGGCTAGCACTCACACAAACGGGTTGGCCAAACCCAAAGCTGTAGAACTGTTGTAGGTTGGGTTCATAAGCATCTGGTTTGAGGTTTTCTGATTTATTGACGACCAACAAAATCGGTTTGTCCTTAATCTTTTTCAATACCTTAGCTACATAAAAGTCATCACTGCTAATCTGTTCTTGGTAAGACACCAAGAAGATAATCGCATTAGCTTGACTTAAGGCTGCTCGAACTTGGACTTCAATTTGTTGTTGGAGCGGGGTCTTTTGGGAAATTAATCCCCCAGTATCAATAAAGGCAATTTGCCGCGTTAACCATTCACCAACACCATAAATGCGGTCCCTTGTGGTATTGGGCGTATCGGAAATAATCGCGTAGGGTTTTTGGATTAAGCGATTGAACAAACTCGATTTGCCAACGTTAGGTCGGCCGATAATGGCAACAGTAAACACCTTAAAGTTTAAAAACTGTACGCGCTTGTTGCACCATTAACTCGACTACTTGCTGAAAAGTAAGGTTAGAACTGTCGATGTAAATCGCATCGGGGGCTTGCACTAAGGGATCAGCAGTTCTGGAAGTATCCACCGCATCACGGTGCTTAATTTCCGCTACTAGTTCCTGAAAGGCCTTGTCACTGTCATATGCTATGCCAAGATCTTGGGCGCGTCGTTGCGCTCTTATTTCCGGTTTAGCATCCAAGAAGTACTTTAACTGCGCGTCCTTCAATACAACGGTACCAATGTCGCGTCCATCCATCACAATGTTGGTGTTTTTAGCTAACGCTTGTTGGCGCAATACCGCCATCTTACGCACTTCGGGATTGGTTGCTAAATTGCTCGCTAGTTGGGCAATTTCCTGGTTTAGGATAACCTCTGAGATATCAGCGTTGTTATAAAACACCTTTTCGTGTTCAAAGCGCCAGTTAATTTGGTTAATCACCTTTAACAAAAGGTTGACATCCAAGCGGTTGACCTGCATCACGTAAGCGAAGGCCCGGTACATTTTGCCAGTGGAAAAGTACTCAAAACCGAGCTGTCGGGCCACTGTTTTAGCAACACTAGATTTACCAGAACTACTAGGACCGTCAATGGCAATTTGAAAATACATAAATTTAACTTAATGGGTGTAAGCAATTAAAGGGAAGTGAATAAGAGGGTTAGTAAAACAACCAAACCCACTAAACTACTAATAAAGAATACTAATAAACTGAGCAAAATCCGCTTAAATAACGGGTTGTAACTGCTCGCTAAGGGCTGGCTTAAATCAGTGGTGGTAAAGCTCACTTTTTCGTTAGTGAGTGCCATTAGTGCCTTATTATAGTTCCCCAATTGCACTACGAAAGTTTGCGTTTGCTGAATAAACTGTTGTTCGTTGTCCTCAAAAAAGCGCTTTAGGTTATCTATTTTATCCATCCCCCGGTCATCAGTAATCCAACTTTCATAACCAGTCGCGCTATCGTGGTGTTTAATTTGTAACCACAAGGCATCAGCTTGGGTGGCGATGGCTTGAAAATCCGATGTTTGGAAGTTGTTAATGAGAGTTTGCAGTGATTGTAAGACCTGATTGGGTTCACTTTTGCGTTTCACCGGATTAGCACTCAATTCACTAAAAGGCTGGTAGTGCGACAGAATTTTCGGGCTCAATTCGTCTAGTTCTACTTTTCAACTAGCGACCTGTTTTTGCGCTTTCAGTAACGCTTTTTGAACAGTCGTATTATTGTCATCGTTCTGGCGGTAAAAGCGAAACTTCTCTAAGCGTCCCATTGCTACAGTGCATTATGCACTACTGTTTGGATGTCATCATCCTCTTCACACGCATTTAAAAAGCGGTTTAAGAGGGTGTGTTGTTCGGCGTTAAGTTCCACCGTTAACAGCGGAATTAAGCGCAACTCACTCGCTTCAATCGTAAAACTGTTTTTAACGAGTAAATCCTTCAACGCAAAGTAACTCGGTGAATCGACCACTACTTCAAAGCTGTCATCATCTTCATTGAGATCTAACAAATTAATGTTGTTACTAATTAACAGTTCAATTAACTCGTCTTGGGTAAAGTTATTGTGTTTAACTAGCGCAATGCCCTTTTCCTCAAAGTTAATTTTAACACTGTTGGGCTTGGCTAGCTGTGCCTTTAATTTAGCAATGTAACCGTTTAAACTACTCAACAACCGGTTCGGATTATCGGTTAGACCAAAGACAATGATGCCAACACCATTGGGACCAAAGATCTCATAGCAAAACTCGCTTAGTTTTGTGGGATCCTTTTCACTGCCATGGATGTTGCGTTTAATTGAATCCGCACTTAAACCATGAGCTAACGCTAGTTCGACTGCCACCTTTAGCTGGGGGTTGGCATCAATGTTACTGCCACCTTTTTTGACAGCAGCAGCGATCCGCTTCGCTAACTTTTGTAGTTGCTTCGCGTTGGATTGTTGTTTTTTGTTTGTTTGACTGGCAATTAAATGCTTCCGCGGCATGAATTAAAACTCATTAGCAATTGCGTCCACTTGTTTAGCGAACATGTCCACTACTTCACTCTTACTTTTGTCCTTGTAAAGTAGCTTGGTACCATCAATGATTACACTAGCAAAACTAGCGACTCCAGCAAAACCAAACAGACCGCGTAGACTGTTGACATGATCGCCCCATGGGTATCAACCTAACGGTGCGCCTTGGGAACCCAAAACGACTACCTTGAGGTTACCCAACATTCCTGATGATGCGCCCTTAGTGACATACTTATCCTTAAAGGTTTTGTTTGCCACAAAGACATGGTCAATAAAGTTCTTTAACGTTGCGGGGTAGTTAAAGTTGGTCATTGGCGCCAAGATAATAACACCGTGCGCTGCTTTGAGCGCATCAATGTACTTATCGGAGTTTTCTACAGAAAAGAAAGTTCCAGCGTTTTGGGTGTTATAGGAAATTTGTCCCACTGGTAGTTCGTTGAGGTTCCAAGTGGAGAGCTCTACGTCCTTGTTTTTAGCTTGAAAGGTAGCTAAAAAGCGTTCTAACAATAAATGGGTGTAAGAACCACTAGGTGATACAGAAGCATCAATAATAATTACCTTCTTCATACTGAACTAATTGTTATTGTTTAAATTCTACTCAGCTTTAGTTAATTTAGCGATTAAATGATTGACTTCCGCTAATTTTTTTTGATATTCCTCTAACTTTAAAAGCTCCGCCTTAACTTTGTGCGGTGGTGCTTTTTTTAGAAAGGCAGGGTTAGTGTTAATCGCTTGACTACGTTGGACTTCCGCTAAATAAAAGCTCCGTTGTTTTTCTAAGTGGGCTAAGTTGTTGGCTGCATTGTCAACGATTTTGATTTGGAAGCCTGGAGTTTTGTTAACTTCTATCCTAAGATCGACCCAGTTAAAGTGGAAGTAGTTTTGCACTACAGGAGCAGCTTTACCACTTAAAACAACGGTTAAATTCTGGTTAGCTACTAGTTGGTTGTTGGCCTTGTAGTGGCGCAAGCTGGACACGGCTTCAACAACATCAGCAAACAGTTTCGGTGGTTTAACTGCTTTGGCAGTTGGCCAACTTGCTTGCATGACACTGCCACTGTGAAACTGTTGGTAGATCCGTTCCGCCAAGAAGGGAGTAACGGTACTTAGCATAACGACCGCCGTACTTAAAACAGTTTTAGCAGTTGTGTACAGCTGGGGTAAAAGTGCCGTGTCCTCTTGCTTAATCGTTTCAATGAAGGTATTGCAAAAATCATTTCAAATAAAGTTGACTAACGCTTGGTTGGCCAAAGCTAACTGGTACTTGTCTAATAACTTGGTGGTTTGGACAATGAGTTGTTTCAATTTAGCCCAGATCCAGCGCTCACTCAATGTAGAGGGTAAGTGAGTACTTGGTACAGTTTTGGCTTGTTCTCCTAACTGCAACACGTACTTGGTCACATTCCACAACTTGTTCAAGAAGTTAGCGGCACTCTTTAACTTCTGTTCGTTGAAGATTAAATCATCACCCGGAGTGTGATTGGAAGTTAAGAATAAGCGCGTGGCATCGGCCCCATAATCACGGATTAAGTCCATTGGGTTAATCCCGTTGTTTAGGGACTTGGACATTTTGCGGTTTTGGGCATCGCGGACTAGACCGTGAATTAACACTTGTTTAAAAGGTAACTGCTTGGTTTGAAAGTACGAGTTAAAGGTCATCCGTAAGACCCAAAAGAAGAGGATGTCATACCCGGTTACCAAAAGATCAGTTGGCACAAAGTGCTTGTTTTTGTGTCAGTCTAAACAAATTAACGGTCACAGTGATGAGGAAAATCAGGTATCGAGTACATCGGTGGACTGGGTGTAGTTTTGCTTATCAGTTGGTGCAGTACTGCCCACATGCAACGCACCACTTTGTTTATGTGTTCACACCGGAATAGGGTGACCCCAAATTAATTGGCGCGATATGCACCACGGTTTGAGCTGACTCAACCACCTTGTTACCTGTTTGTTAAAACGCTTGGGAATTAACTCGGGGTATTTCTTAATGGCGAGTGCTTTTTTGAGTTTCGGTAAATCGACAAACCACTGCTTGGATAATAACGGTTCTACGACCGTGTCACTGCGCTCGGAAAAACCAACGTTACTGGTTAGTGGCATAGTTTTCACTAGTAGTTTTTGCGTTTGTAAAAACTCGACTACCTGCTGACGTGCTGCACTTACGGTTAAACCAGTAAATTGCTTAGCGTGCTCATTTAACAAACCATTGTGGTCAATGCAACTTACAAACGGTAAACGGTACTTTTCGTTTAAGGCAAAGTCGTTAAAGTCATGGGCGGGGGTGCACTTCAACACACCGGTGCCAAACTTCTTGTCGACATAACTATCGGTGAACACTGGCATTAACGCACCAGTGAGCGGATTAACTACCTTTTGTTTGACAAAGCTGTGGTAGTGCTTGTCTTTGGGATGGACAAACAAACACACATCAACAAAGATCGTTTCTGGTCTAGTAGTTGCCACCACTAAACGTTTTTTGGGATTATTAGCTAACTTATAAGCAATGTAATACAGCTGTTGGTCAACTGGTTTATTAATGACTTCAATGTTCGAGATGGCAGTGTTTAGCTTGGTGTCCCAGTTAACCAAGGTTTCAGCTTGGTAGATAAAGCCCTGTTCAAACAACTGGATGAAACTATCGTTGACAATTTGACTCGCTTTTTTGGACAGGGTAAAGTTAACCTGGTTTCAGTTTAAGGATGCCCCTAAACTTTTGAGCTGACTTTGAATGGTGTCCCCTTGTGTTAAGGCCCAATCCATAATCATTTTGACCTTTTGTTTACGCGGCGCTTGGAAGTACTCGGGATTAGTTTCCCGTGCTAACTTTTCGTATTTGGTTTGCGTAGCAATCCCCGCATGGTCAAATCCGGGAATCCAGTTCACGCCATAACCCCGCAGTCGCTTAAAGCGCATAATTTGGTCAGTGATACTCACTTCAAAGGCATGACCAATGTGCAGCGTACCCGTTAAGTTTGGTGGGGGGAGAATGGCAGTGAAGGGTACCTTGTTTTTTTGTGGTTTAAAAAAACTAGCACTGCTTCAACTATCATAAAGACCAGTGCTCACTGTTTTGAAGTCGTATTGACCTTGAAAACTAAATTGCTTGTCCATCTGCTTTAAATAACTCAAAGATTTGTTTGTGCATTACTTGGAGGTTATACCCGGTTTTGGCACTAACAAATAAACACTTATTAGGATTGACTTTTAAAAAGTGTGCCAGTTTATTCTGGGTATGAAAGCGCTCGCTTTGGTTGAGCTTATCAAACTTATTGGCTATCACCAAAAAATTTAACCCCGTTTGGAGAATGGTTTTGACCACTTCCTGGTCCTGAACGGTCACTACACCGCTGTCAATCACTAGTACCACACCAGTTAAGTTTTGGCGAAAATTGAGGAACTGCGTTAATAAATTAGTAATTGCTTCCTTTTGCACTTTACTCAGTTTAGCAAAGCCATAACCTGGTAAATCGACAAAGCGTTTACGGTTGTATTCAAAGAAGTTTAACAGTTGTGTACGTCCCGGCGTCGCGGAAGTTTTAGCTAGCTTTTTTTGAAAAAAGGCATTAATGAGGCTTGATTTGCCGACATTACTCCGCCCCATAAAACAGACTTCCTTAACACTGTCTTGGGGACAACTCTCTAAGTCACTTGCACTTTTTAAAAAACGTGCCTCCATTTATTAATTACTTCTGGTTTTTAATGGAGCGCATAATTTCCTTGACTTGTGTTTCAGAAAGCTTACGACCCATTTTGGCATACATCGCCTTAATTTGGTTTTCGGTGATTGGTGGGTTATCACGAATCTGTTTTTTAAAGATTTTGATAGAAATAAAATAGCCAATAATGACACCGACTAACAGGGAAAGGGGAATTCCCAAACCTAAGGCAAGCGCAAGATCATTCATATCTATATCTTAATCTTAATCCTCTCTAGAATCTTATAAAATCAGAATAGCGTAATTAATGAACAAGCTAATTAGCATTCTGGTTCCTTGTTACCAATCCCAGCCCTTTCTCGATCGCTTTTTCAAATCACTTTTAAAGCAAGACTGGAACGGTGTTAAAGTAATCTTCTTTAATGATAACAAGCCAGATCCAACATACGAGATCCTAAAACAATTTCAACAAGCACATCCACAACTAGCGATTGAAGTACATTGTGGTGAGAAAAATGTCGGTGTAGGCGGTTCACGTGATCAACTAATAAATTATGTAGACACACCCTACTTTTACTTTGTTGATCCTGATGATGAATTTAGTGATCCCAACTGTTTTAAGGCAATAGTGGAAACAATTCAAGGAGAAAACTTTGATATTGCAGTGTTAAACAGTATTGTTTACCTCCAAATGTTGAAGAATGACTTTTTAATTAAACACATTCCGCTCAAAAACATCTTTCAGGGCAAGGTAAAACTCAATCCTGATAACACCGTAAATCATTTGCACTATATCCAGAATAACGACCAGTACATTTGGAACATTGTCATCAACACCGCCTTTTTCAAAGCATTGGATTTGCAGTTTGTTAACCGCTTTATTGAAGACATTGCGGTGTGGTTTCCGATTATGTTTAAAGCCCAAAAAGTGCTGTGAATTGATGTAAATGGGGTTAACTATTACTTGCGACCGAACAGTGCTTCCACCCAGAAAAACAGCATTAAATTACTCTCCTTTATTGAGGCTTACGAACGGTTGTATTTCCACTTAAAGAAGGTGGGTAAGCTAGCTGATTTTATTGACCCTAACAATAAGATTGAAAGTCGTTTTTGACGGCGTCAAGCCTTCATTTGGTTTAGTTTTATTAATGTTTCATGGATGAAAGCGGAATTTGAACAGACCAAAAGTGTGTTGCAAAAACTGTTTGACTTCATGGAAGCCAACGGCATCTACGACAGGGTATTTACTAATAAGCACCACGGTATTTACTTATTGTGAGTTAACCGCTTAAAACACTTTAAGAAGTTAGTGCAAGCACAACCACATCTATAAGTTTTGTGTTCTAACAAGCTAAAGCTTCGAGTAGCTTTTTAAAAAGTTGTTCTCTTTCTAGAGTTTGTTTTCCATCTTGGGCTTGTACAACCATTTCCTTTTGATTAGCGTGAATCTTTATGTAAAGTGTGCGTTTATTATCTTCATTGGCTTCGTTAAAAGCTACAAATGAAATTAGCTCACTTTGATTGAGTGAATTCATTGCGTGATCAATAAAAGGTAGTTTGATAATTTTTTTATTTTTCATAATTAATATTTGTTTTTTGGAGATTGAAAAAATAGCTACCACTTGCAACTCGGTTGCCAACAGGGATCAGACGTTTTGCTTATCAATTAAAAAAATAAAAGATTTGCATTTTGCTCCTCCACTAAATAAATTGAACTGAGACTTTATATGTGACAACAAAATTACCAAAAATTTTTTTGAATATTTTTTAGATAAATAAAAATACCAACAACTGACATATAAAAATCAACTGCTGGTACTTTTATTTTGGGTTGAATCTACTTAGACTCCAAACGATCCAAACGTTTGTTAATTACTTGAAGAGTTTCAAGAATTAACTTAAGCGTTTCCCCTTGGGTTTGAATTTGTTCGCCCTGCTTTTCAACAGTTTCAGTGAGCTTATTTATTTGTTCACCCTGAAGAATTGAAACTTTGATTAGCTGACTAATTTGTTCACCTTGAGAAACCAAAGTGTTCTCAATTTTTATCAGCCGCTGATTGTTGGAATTTTTGAAATCATTAAACTGCTTTTGGGTAACGTAACCACTTTTTCGTTTTTCCATATGGTTTAAATTGCCATAAAAGCCCATGTCATAGCTGCCATCAGGTAGATCTTCGATTCAGGCTTTTGATGTTTTAATCTTATCTTTTTTCAAATTTCTTTTAAACAAAGTAATTGAAATTTCACGTTTAATGATCAAATCCTCCTTTCTTTACAAGATTTAAAAAACTACAACGTGCAACAATGTTGCCAGAGGATATCAACTGCTTGGTTGATCCAGTAATTTTCTTGAAATACTTCATAACGATCATTTCACTAAATAAATTGAGTAGTTGATTTGGAAGTAACAAGTAGAATTTCTGAAATTTTTGAAGAGTCAAAAAATCTATTCATGAAATTAATTGAATGAAATTCAGGTAACAAGGCCTTTTCAAACTAAAAAAGCGCTTTTGCCGCCCTACTAATTAATCAATTGCGTAAATCAAATTGAAACGCCAAATTTATTTAACTCACCTCCCCCAAATCTTTCTTAAACTCACTTAACTTCCCCGCAGCAGCCAAATAATCCTTGTACTCGGATTGGATGTCGGTTAAGGCCTTTTTAAAGGTGGGGTTATCAGTGAAGGGTTTGGCCAAATCCGCACTTGATTTCAACAGCGCTTTGGCTAACTTAACCACTTCCGATTCTTGGGTATCAGTCGATTGCTGTCTGCTTTGCTGGGTAGTTTGAACGCTAACAGAAGCTGCTCGGCTACTCCCCTGCACACTGCCCTTAACTTGGGTCCCCTGACCCGAACCCATCATCGCCACCAAAGCACCGTACAACTTCGCGGTTAACCCCGTTTGTGCTCACGTTTGGAACAGGCTGCCAGATTCCAGTAAGTTCAAACTAAACTCGACGCCGTTTTCCTTAAAGTTATTACTCTTAGCTTGTAAATAGCGGTACAGCAAGATGTCGGCTTGTTTGGTGAAATCGCGTTTGTTGTTCGCCAAAAAATAATCACCACCGTCAATTGCCAGGGCGTATGTACCATCGTCACCACGGGCGTAAACAGGGGTTTGGAGTGTGGAGGTGGTTTGTTGAACTTGTTGCTGGGTGTGGGTAAGGGCGGCACTCCTTGGGAGCGGAATTAAAACAGAGTTGCCCCCGGAGCTACCACCAACATTGGTACCACTACTAGAATCTGCCCCAGTGGAAGCTGCCGCCATTGCTTGACTACTCGTCAACCGCACCGCGTCTTGGAGGTAGTGGTGTTTATCATTGGAATTATTACTACCCCCATTAGTTTGCCCGCCGCTCAGTTTGCGCTGGGTTGTGTAAAACGCATCGGTTTGATTATTCTGCTTCACCTTGACAAACGCCGGGTCGACCTTAAACCCGGGGGTTTGGTCGGTCATGGCAATTTTCGCACGCGAAACTATGGATCTAATCGCCACGGCATCCTTCCCCTCCATAAAAAAACTATCCAGCGGATTTTGGTTGGACTTATTCGAATCTACCTTGACCTCGGGCAACGAACTCGTCTTACTCCCACCCACCAGGTACTCGTACTGGTCAATAATGGCCGTAGCTAACCCCACGCCCTTAAAGGCCCCGCGCAGGTTGGACTTGGTCACCAAGAGCTTATTGGCAGCGCCATTGCTGTTTCACTTGGTTTGCCAGTCAAACAACCCGGTGCGGGAGGATGTATTGGATTGTTGGGTTTGACCATTCTTCTGCTCCGGAAACACCGCCTTAATGAAATCCCCGAAGTTTTGGGCACTGTTGGGGGTGGGGTCGAAGGTGGTTTTACCTTGGCTTTGTCCATTATTGGGCTCGCGAAACGCGGGGAAGGCATAGACCAACTGTAAGGCACTACCAATCGCGGCTTGGTCGTATAAGCTGTCGAGCCCGTCCTTGGGTGCTTGGTACGCGAAGAACGCTTGGGTGAGGGGAAACAGATAGAGCAAAAGCTGTAAGCCACTTTATAATTAAGTAAATTATAGCAACGCTGTCAAAGCGTACTTTTACCATTGATTATTTCCTTAAAAAGACTTGTTTAAATGACCAAAACTAAGTTTAATCCGTACCAATTTCGCAAACAATTTAAGTGGTTTAAAAACAACCCCCAATGGGTTAACTTTGACAACGCTGCTACCTCAATTGCGCTAGACACCGTTAGTCAAGCTTGCAAGGAATACTATGAGCTTTTTAGTGTCAACCCCCACAATAAAACCCCTGATCTCAACAACCAGATTATTGCCATTATTGCTGAAACCCGTCAACTAGTAGCGGACTGGTTTAATGTCACTGCACCTGAAATTATCTTTACAAGTTCAGCCACGGAGTCCATTAATTTGTTTGCCCATGGCTTAAAGCCCTGGATTAAACCCGGTGATGAAATTGTCTTAAAGGGTGATGAACACTCGGCTAACGTTTTGCCCTGAGTGGCACTAGCTAAACAAACAAAGGCCAGACTAGTATGGGTCGAAAAGCAAGCTAACCAATCCTTAGAAGACACTTTTAAGTCATTGATTAACCCGAAAACTAAAGTAGTAGCCATTACCGCTACTTCCAATTTGTTTGGCAATTCAATTGACTTTGCTCAAATTGCCGATTACCTCAAACAAGTCAATCCTAAAGCTTTTGTAGCCGTGGATGCAGTGCAAACGGTGCAGCACAAGCAAATTGACATTGCCAAAACCCAGATCGACTTCCTAGCCTTTTCTACTCATAAGTTTTACGGTCCCACTGGACTGGGGGTAGCTTACATTAAAAAAAGCTTACAACCCCAATTACAACCCCTCAAACTCGGGGGTGATATCTTTACCCAGATTGACCCGGACAATACAATTCACTTTAAAAGTACACCCCTAAAGTTTGAAGCCGGTACCCCCAACATTATGGCCATCTATGCCTTAAACAAGCTGTTGCGCTTCTTTAAACAAAAGTTTAATTTTGCCCAAATGATGGCTTATGGTCACCAACTTAAACAACAAGCTTACGAACTGTTAAACAGTAATCCGCAAATTGTGCTGGCCAACCACGACCAGGATGTCCCGATTTTTTCCTTTAAACACCGCCAATTGGCTACCATTGATCTAGCCACTTTCCTAAACATCAATAAGATCATGGTACGCCAGGGCTCCATTTGTGTGGGACGTTACCGCAATAAGGATTACTTTGTGAGAGTTTCGTTAATGCACTATAACACAGTGAAGGAGTTGCAGTATTTAGCTAAACTCCTAGCAACTGATACCAAGACAATTATTAAAAACGTAATTAAGTAACTATGGATCGCAAAATAAGGGAGCAAATTATTGCCATTTACAGCAACTTACAACACAAACAGGACTTAACTCAGTTTGCCCATTGTTTAACCACTAAGGCTGATGATAACTGTGAGGACTTCTTTAACTTAGGCTTACAGTTTCAAAACAACCAACTAACAGCACTTGGTTTTAACGGGGAGGGTTGTATTATTTCGACAATTGCTAGTGAACTGACTTTAAGTGCGTTGGAAGGCAAAACAATCAAAGAAGCCATTGTCTTGTTAGAACAGTTTATGGAAGCTGTACAAACTGGTAGCTTAAGCACGCCACTACCCCAAGCTTTACAACTGTTGTGGGACTTTCAGATTGACCAGAAGCGTTTAAACTGCTTACTCCTAACACCACAGAATTTGCTACAGTGGCTTAAAGATTTTTCGCACTAAGCGCTAGCGTTTGCCCTAACGCATCATACAAGCGTTTGTCACCCACCAGGATTTGCTGGTGACTACGTTTGTTTTGCACCTGGTTAGCGCGCACTAAGTCTAAAAAGGCTAACTCTTGCGTACCAGCGTCCAAGGCTTGTTCTACTAGTAAAGCCATAAAGATTTCCTTTTTGAGGCCTAAAGCACTGTTCTTATCAGTTTTTCAACTAGCAGAAATTTTGTTAACGTCCTCGTTGGTAATCTGAATGACATAGCTCAAAGTCTTGACACTCGAGTCCTTGGTGAGTGGTACGGCTTTGGCTTTCTTATAGTCTTCGTTACCTTGGGAACTACTACTTGAATCAGACATCTTCACCTTGGTTTGGCCAACATAACCTTCGAACTTGTTAAAGTAACCATCATCCAGTTTGTCCACTACATCAACCAAGAACTTCTTCTTGTTGTCAAAGCTAATCAACGGTTCCCCACTGGAGTTAAAACGTCCTAAGTTACTGGTATCGACATTAACTGCACGGTGCAACACTTCACCAAAGGCATTGAGCTCGGCATCGGAATTAGTGTTTTGGATTAAGCGTTTCAGGGTTGCTTTATCCTTGTACTGGCTCAAGGCCCCGGATTTGTCACTACTTGTTACTAACTGACCAAAGTTGTTAAAGATTTGTTCCGTGAGTCCGGCACTAGCAGTCGACACACTTTGGGGCGAGTTAATCGTTAAACCGTTAAAGCCAAAGAAGTGTTTTTCGTTAGTGGTACCACCAGTAGACCTCTTTTGACTTTTCGTCTTATCATCAAACGGTTCCGCGGTAAAGTTGTAAGTGCTTTCGCTTTCCAGTAACGAATCGGGTTTAATTGGCGTACTCTTATCGTTAAATCAGTTGGTTTCCGAACCCTGGACATAATCGGGGTTTTGGACAAACTTATTGAAGGGGTTGTCGCTTTGAGTATTTGTTGCCTGAGCTGTCTTTAATACTGCTACTTGCGCTTTTGCTGCGGCTGCTGATGCCGTAGCCGTATCATCATAGCTCACCGTTCACGACACAAAGGCACGGGTACCCACGGTAATCGCTTGTTGGAGCAAGCGCTTATAGTTCTTCGCCCCATCCTTAACTAGTCACATTAAGGTGGACAAGAAGCGCAGGTACTTGTACTGCCCATCACTACTAGATGCTTCCAGTTCACTTTCAAACGCTTTTTTACCGAGTTCCAAGAGGGTTTGATCACCAGATTTTTGCATGCCACTTGAAGCTACAGCATTTGCAACTGCTGCGCTACTTGAAAGTGAAACCATTCCTGATTGAGCTGCTTGTGCTTTTACACTACCCAACTCCTTGTAATAACCAAACTTTTCCTTGGAAGTGAGTCGTTGTCAGGAACGTAAGTACAAGGTTTGTTTAACGAGGTCGTTAAAGTCCTTGTACTCATTGCCTTTAAGTTCTTTCTTTTCAGAATCGTAATTGTTCTTAAAGTCGTTGGTATTAGTAAAGAACCACTCCTTAAAGGAATTGAGAATCTTGGTTCACTTAATCCCAACATCAGACAATAAGGCCAAGATCATGTTGGTCTGGGGCTTATTGTCCTTACTCTTGAGCGAAACAATTTCGGAGAACTGACTACCAATTTGGGGTGTTGCACCTCATTTGGTGATTAAACTAGATGCTTTAGTTTCTACATCACTAGTTAACTGCTTTAACTTTTTGTCGTTGTTTTCCCGAGTCGGTTTGATGTCCGGTTGTTTAACGGTGTTTGTGCTTTTTTGACTATCTGTTGTCTTTAGTGTTACTAAAGCGGCAGATCCTGATTCAGATGCTTTTACTTTTCCATCTGCTGCACTATCTTCCGGAATAAAGAACTTCTCTAAAGCGGGGTAACTACCATCACTAGCGCGTTGGTAAGGGAGCGGTTGGCCCAAGCCAATCGCACTCTTTTGGGCTTGTTTTTCTAGTTGAATGTACTTATCAGCGCGGTCAATAATAGCTTGCTGTAAGCCACTCACCTCCCCCAAATCTTTCTTAAACTCACTTAACTTCTCCGCAGCAGCCAAATAATCCTTGTACTCGGATTGGATGTCGGTTAAGGCCTTTTTAAAGGTGGGGTTATCAGTGAAGGGTTTGGCCAAATCCGCACTTGATTTCAGCAGGGACTTGGCTAACTTAACCACTTCCGATTCTTGGGTATCAGTCGATTGCTGTCTGCTTTGCTGGGTAGTTTGAACGCTAACAGAAGCTGCTCGGCTACTCCCCTGCACACTGCCCTTAACTTGGGTCCCCTGACCCGAACCCATCATCGCCACCAAAGCACCGTACAACTTCGCGGTTAACCCCGTTTGTGCTCACGTTTGGAACAGGCTGCCAGATTCCAGTAAGTTCAAACTAAACTCGACGCCGTTTTCCTTAAAGTTATTACTCTTAGCTTGTAAATAGCGGTACAGCAAGATGTCGGCTTGTTTGGTGAAATCGCGTTTGTTGTTCGCCAAAAAATAATCACCACCGTCAATTGCCAGGGCGTATGTACCATCGTCACCACGGGCGTAAACAGGAGTTTGGAGTGTGGAGGTGGTTTGTTGAACTTGTTGCTGGGTGTGGGTAAGGGCGGCACTCCTTGGGAGCGGAATTAAAACAGAGTTGCCCCCGGAGCTACCACCAACATTGGTACCACTACTAGAATCTGCCCCAGTAGAAGCTGCCGCCATTGCTTGACTACTCGTCAACCGCACCGCGTCTTGGAGGTAGTGGTGTTTACCCTGGGAGTTATTATCCCCACTTTGCCCGCCGCTCAGTTTGCGCTGGTTTTGGTAAAAGGTATCGTTCTGCTGACTCTGCTTCACCTTGACAAACGCCGGGTTGACCTTAAACCCGGGGGTTTGGTCGGTCATGGCAATTTTCGCACGCGAAACTATGGATCTAATCGCCACCGCATCCTTCCCCTCCATAAAAAAACTATCCAGCGGATTTTGGTTGGACTTATTCGAATCTACCTTGACCTCGGGCAACGAACTCGTTTTTTTCCCACCCACCAGGTACTCGTACTGGTCAATAATGGCCGTAGCTAACCCCACGCCCTTAAAGGCCCCGCGCAGGTTGGACTTGGTCACCAAGAGCTTATTGGCAGCGCCATTGGAGTTTCACTTGGTTTGCCAGTCAAACAACCCGGTGCGGGAGGATGTATTGGATTGTTGGGTTTGACCATTCTTCTGCTCCGGAAACACCGCCTTAATGAAATCCCCGAAGTTTTGGGCACTGTTGGGGGTGGGGTCGAAGGTGGTTTTACCTTGGCTTTGTCCATTATTGGGCTCGCGAAACGCGGGGAAGGCATAGCCCAACTGTAAGGCACTACCAATCGCGGCTTGGTCGTATAAGCTGTCGAGCCCGTCCTTGGGTGCTTGGTACGCGAAGAAGGCTTGGGTGAGGGGGAGGGGGTTTTCCTCCACTACTCACTGGGCAAATAGATAAGCCTGGAGGTTAGCAAAGAACTCCCCGTTAGGATCATTTGGTTTGGAATTAACAAACTTGATTTTGTTGCTAGTACTGTCGAGGTTATCTTTGGTAGTGGGTTTAAACTGATCACTACCATCAGTCAACTGCACATTAGCAACAATGTTGCTAAAGATAAGTGATTGGAAGTCGGCTATTAAACGGTCGTATAACTTTAAGGCCTTTCAGGAACTTTCACTCCCACCCCACTGGTCGTATTCCTTTTGGAGCTCTATTGGTCAATTAATCGGTTCGGCCTGTTGTTTTTGGTCCAACAGGTTATCATGTTCATCCTCCACGTTTTCACTAAAGATAGTTAAACGGGTAGCAATGTTGCGATCCTTATTGTTTTGGTACCAACTCTTTAACGCTTCACCAAAGTTAACCCTTAAAATGGTTTCCGTACCATTGTCCTCAGCTAAAGCGGCTTTTAGTACAGCTTCAGGTTTAATGATGTTGTTTTCGGTAAACCCAATCAACTGCTTGGAGCGGTTTTGACGGGGAAACAGATTGGAGGTATTGAGTGCAGAACACGCCGCTAGCACCACCCCTAGGCCAATTCCGGTAAATGTAAACGCAACTAAAGGTCATCGCTTTAACTTATAACCCATAAATTGTGTGTCCGTTAAAGAATAATAGTTTGCTAATTATCTCATGAATGGCACTAATTTAGCCATTGTTTATCCAGCTAATATAAATAGGTGAAGGTGAAAAACTTCAAACCGCTTTTACAATTTTGTGAGTTCTTTTGCCCAATTAAAACATTGGGCAGACCAGCATTAGATTGCTATGGTATATTTAAGCGGGACTTGGGAACCGTTAATACTATCTCATTTTCCTCAAGATACAACTGTTTCATTTTTTCGTAACTTTTATATTTTGTAAACGTTCCTCTTATCATTCATACATGTCTTTATAATTCCCAAGTTCTAAAGAGTCCCTTCGGGGACTTTCTTAATGCTAAAAAAGCAGTTTGATGCTACTTATAGTCGCTATAAGCCATCTAAACTGCTTTCTTAGTTTTGATAGTTATTCCTAATTTGTGTCTATATATTATATATAGTCCTTATTAATAACTATTTTTATATTTACCGGCGCTGTGGGGGTTGGCTAGCTCGCTGGGGGTGGCGGGCTGTGGATTTGCTTAATCGTGAGCTGCTTAATTTGTTCAAACAACTCTGGTTTTTCCGCCAACACGCTAAAGAGCTTCTCTTTGCCAATCGCAATGTTTTGGCTCTTAAACGAATATGCATTGTCACTGCGCACCACCACATTGTGTTTAAGCGCTAACTCAATCACTTCGTGTTCATACACAATACCGCGGTTAAACATAATTTCCAAAAAGGCAACACCAAACGGGCGCGCTACCTTATTCTTGGACACGGTTAACTTACTCTTAATGCCGACATAGTTACCAAAGCGGTCTTTGAGCAGTTCACTGCGTTTGGCCTCCATCCGCATCGAAGCGTAGAACTTCAACGCCCGACCCCCAGTAGTTACTTCCCCATTACCAAACATGACTCCGGGTTTTTCCCGGAGTTGGTTAATAAACAGTAAGCAGGTGTCAGATTCAGGTAAGAGTGACTGCACACTGCGTAGTCCCTTAGACATCATCCGCGCATGGAGTCCTATTGTCTGGTCATCCATGTTGCCCTCCAGTTCTTGCTTAGGAATCAAAGCCGCCACGGAGTCCACCACAATTAAGGCCACTTTGTTGGTCTTAATCAGTGACTCCATTAGGGCAAAGGCGTTTTCCCCATGCTTGGGATGGGCTACTAGTAATTTACCCAAGTCAATCCCAATTGCCTTGGCGTAACTTAAATCTAGTGCACCCTCAGCATCAATGTAACAGGCCACTTTGCCCGCTTTTTGAAAGCTAGCGACTGCATGTAACGCGACCGTGGTTTTACCCGAGGACTCGTTACCATAAAGTTCTACAATGCGCCCTAGTGGCAAACCACCAGTGCCGAGCGCTTCATCTAAATGAAGGCTTCCAGTTGAGACAGTTTTAATTTCACTGTTTTTCTTTGCATCTAAAAAATCAAAGTCTGCCAAGTTATTGTTTTGAATAAAACTGTTGTCTTGGCTAATTTTCTTATTAATCATTTCTTTTTGTACCATTGTCGTTTACATCATCTAAATTGATTCAAAAAGGGAAAAACGCCATTTTTTTAGATAATTAAGGCAAATTTTTATGAAATTAACACTTTGGCTGGTGTTGGGCGCTGTCGGAGTCGGTGCAGTTGGTACGGGTGTGGGCTTTGGTACCAAGTACTTTTTAGACCACAAAGCCAAAAGTAACGTGGACGTTGCGTTTAAACAACGTTCACTACGCAAACAGGTCAATGTGGGCTTTTGGAATGCACTCAACTTCTTGGGTAGTGAAAGAAGTTTCGGCAACCAAAACAGTAAGGTTAAAACCAAGGGGATTGCTGAAATTATTAACCTCCTTAAGTACGACATTGTCGGTTTAGCGGAAATTCAACCATCCACTGAAAGTACTGCTGAACGGTTTGTCGACCAACTCAACGAGTACGCTGATCCTTATACCTCCTGGTCTTATGATCTATCTCCCGTAACTACCAGTAAGACTGCTAGTGAAGGCCAGAAGGAACGGATCTTAATTGTTTACAACAGCCAGAGTGTCAAAATGGAGGGACAAGGTTGGTTTTATGACAATCCCGAAATGGACATTAGTAACTATGTCAGTGATGGGAGTTCTTCCAAAACAAGAACACCTAAGAAAACTAAAACTAGTAAAAAGAAGCCTACTAAAAAGAAAAGTTCAAGAAGCAAATCTTCCAAAGGTAGTAAGAAACAAAAAACTACCAATGAAAGTGAAAGTGAAACTTTAGAACTCAAACTGGAACAACGCCGTGTAACAACACGCTCACAATCAAAGCAACAAAAGGGGCAAGAACAAGCAACTGATCAAACTGATAGTGAGGGTGTGACTACTGAAGAAGGAGCAGATAACACTGACACTGAATTAGTTGAAACCACAGCGGAAACAACCGAACAAGAAGCTACCACGGAAAGTACAAAAGACACTAAGGAAACTAAAGATAGAATCAAAGTGGACTGATCGCGTCCCCCTTACAGTGCTGAATTTAGTACCCAAAAGGGCAAAGTAGTAGTGGCTTTTGGTCATTTTGACTCTCCGGGGGCAAACACTAAGCGGGGTGAAAAAATCGCTAAGATGGGCAAGGGTCAAGGTGCCCATGAATACTTTGAAGCGCAAACTACCTTCAAGGCAATGGATTCCATTAAGCAGCACTTTAACAACGAAAACATCCTGTTTATGGCTGACACTAACATTAAATTCGGTAATCAAGCCGCTGCTTTTGGTGAAAGCAAAGATTACACCTTTTTAACTGAAGATAATGAAGCGTGAAAATCTTCATTGGGTACAAAGAGTGGTTATGCCAACCCTTATGATAAGATTATTACCTCTAACAGCTTTAAGAATAAGGTCTTGAATCAAGCTGAACCCTACTGAAATTACTTTGACCGTTACTTAAAAACTAAGGGTCACAATAGTTATCTGTTTGATCTAGCTAGTTGGGGCACTAAACCTAGAACTATTTCTGACCACGCCCCTGTAGGAGTGTTGTTTTAAGTTAATTAACAGCGCAAAATATAAACATGGCGGATAAATGTGTGACCCTTTTTGGGTTCCGCCATTCATAAGGGTGCTCGGGAAACCGGGAGTTTTTTATTGCTAAAGAATACAAGTAAACGGCTTTTTAGGCAAAAGCTATTTAACTCTCTTTAAAGCCAAGTCCCACTGCTGTTCGTAAAACTGCTTGGCTTGTTTTAACTGCGCCACATTTTGCACGGTACTTTCAGCAGGATCATTTTTCGATCACATTTCAATTAGAATTGGTACTGTTGGTACATACTGGTGAATGGCTTTTAAAGCTGCTTCAAATTGGGCTGTACCTGTGCCAAACGGTACTTCCTTAAACTGCTTGGGTAAAGTATCTTTAAAGTGAAAGCCCACTATCTTGTCTTGACCTAATGCGATTTCATCAAAGACTGCCGTACTAAATTGCGATAGGTTACCAATATCCGGATAGAGCAAAATGTTAGCCTTACCAATCCGCTTAATCAAGTTTAAACAACGCACAATCGTGCCAGCAAACGGCGTGTCCATCACCTCAAAGGCAATGGTAACAGCGTACTTTTGCGCTAACTCTGCCACTTTCCGCATGTTAGTAATGAAATTGCGTTCTGTTTCTGTGTCATGTGGCTCATAATACACATCATAAGCCGCTAACTGGATAATACGAATCCCTAGTTTGCGTGCTAACACACAGGCCTTTTCAAAAATGGTTAAGCCCTGTTGGACAGTAGTTTTATTCTTGGAACCCAAAGGAAAGCGCCGGTGGGCACTTAAACACATTGAGTTTAAACAAAAGGTTTGTGCTTGTAACTCATTGTGGAGTTGGTTAATTTCGGTATCACTCCAATCCAAGCGTTGTAACCTAGCATCCGATTCATCAATGCTCAACTCAATAAAGTCAAAGCTAGCTTGCTTAGCAATAGCAATTTTGTCCTTTCAGGCAAACTTATTGTTAATTGCCTTTTCATAAACACCCAGGAGGTGTTTATTTTCAGTACTTGTGAAACTCATCTTTAAATTGTTGTACGGTTTGAAGTGGATCACTAGCATCCCTAATGGTACGCCCAGCAATAAAGATATAAATGGGAATATCCTTAAATAACTGGATGTCGTTGAGGGTAATGCCGCCGGTAACGGTGACCTTAAAGCCCAAATCCGCTAAGCGTTTCACTGCTTGCAAATCCTTATCTGATCAGTTGACTCCCGCTGCTTGGGCATCACGGGAACGGTGTCACACTACCTGCTGAACTCCCACTTGTTTTCACTGCGTTACCTGCTCCCAAGTGAAGTTACTGGTCATTTCCACCTGGGTTTCTTTTATGGGCGTGTAACTGTTACCCACAGTTACCACATCCTTTACGGTGGGCAATTCCGCTGCACAAATGACCGTGGTAAAGTGTGCCCCAGCATCAAAGAACATTTGGCTAAACACCTTCCCCGCATCAGCAATCTTGCCATCAGCCACTATTAACTTATGGGGATAGCGTTTGGCAATTTCTTTAACAGCATTAACGCCCTCCGCTGTTAGTAAGATAGTGCCCACTTCAATAACATCAACAGCATCCCCGACTTTAGCCAGGTCATTTAAAGCACTCGCTAAACTGAGGTTATCAAGCGCAATTTGGATTAAAGGTAAAGCCATATTAAGTTAAATATTTGTGTAAGTTAACCCGATCAATCACAGCAAACAAGCTGGTCTCATCACAACAGTCCAACAAGTCCTTAACCACTTGAGTATCTTCTACTAGGGCCGCAATTTGTGGTAAAGCTTCTGTCGTATGAATGGCAGCGCTGGTAGCCGCTAATCCAATTAACAAGCGCACCGGGCGGGGATCGTTGTCAAAGTAAACTGGTTCCTTTAGGGTCACTAAAGAGAAGCAGTTTTGCTGTACCGTACCGTTATTCTGGGCATGAGGCATGGCGACATTTTCCGCAATAATGAAATACGGACCGTACTGAGCTACACTCTTTAGAATGTCATCAAAGTAAGCTGGTTGGACTAACTTGGCAGCAATTAAAGGATGGATGCAGAGCTGTATCGCTTGTTTTCAGTCCTGCGCGGTTTGGTGCAACCGCACGGTGTGGTGTTGCTTTAAGACGGCCTTAAAATCGAGCTTTGTCATCTTAAAGCACCTTACTTAACGCTTGCTTAATTTCGTTTTCATCCATCAGGTTTAACACCCCAACAATCTTGGCCTTAGCATTCGGGTTAAACTCACTCGTTAAGTGAATCGAGGAAATAATAATGTCAGCGCTGTGCTCCATCCCCTTGGTTTGTCCCATTGACAAAGCTTCCACTTTGGCGGTGTAGCCCAACTCCTTCATAATCTTTTCAACCTTAATCTTGATTAGCATGCTCGTACCCATGCCGTTACCACAAGCAGCAATAATGTGTAATTTCTTATTTTCCATGGTTAGTTATTTAAATCTAATTGTGGGTTAATTTTGAGTAATTGTTGTAATTTAGTTACTTTAGTTTGCTTACCATTGTCAATAATTTGTGCCAGCAAGATTAAACCAACGGTAGCCAAAATTACTAAGATCCAACCACCAATTACATTGTAAGCCCCAATTCAAACTAACAACCCAAAGTAAAGATTCCAGTCAGCCATTCCAATAAAACCGTTGTCAACGGGTGATGCACTTTTACTGATCCACTCAATTGCTTGTGCTAGCGTACTGTTAACAGCTTTTAAACTGTTAAAGCTATCGGATTTAACAAAGCTTTGCGCTTCGGTTATCCGGTTAACACCAGTAGCATTTACAACCTTTTCTACGGCTGTTTTTAATGCCGAACCGTTGTTATTGTCCAAACTATTACCCATTAACGCCTTTAAATCAAAGCTTTGTTTAGCATCTAACAGTGCCGAAGCCTGGTAAGCGTTTGATACGGTGCGCAGCGCAAATGAAATCACAATAATTTCTAAAAAGCCAATAATACCCGGGAGCAGTAAGGCCGCCTTCCAACCACCCTCGGCATTAGCATACACGCCAAAGGCACCACTGTTAAAGAAGAGTGTAATAAACAAAGGAATCGCCACAAAGCTAAACTGGTTACCCGGAATATAGCTAATGCCAGCCATAATGGCAACAGCTAAAAACTGACCAATGACCCCACTTAAAAACCCAAAGGTCACGGAGTTAATTGAAAACCCATAAACAGCTGCAATATCAACCGCTACCACGGCCCCTGGTACTACCTTTTCACTAATACCTTGAAACGCCTGTTGTAACTCAGTGATAAACATCCTTACCCCAGTCATAATGGCAATTAAGCTGGCCACCATTTTCAAAGCACCCCCAATAATGTTGAGCGTAAAGTTAGCTCCGCCAAAGGTAGTGTTTCAAATCTCTAAACCATTAATGGCACCAAATTTGTCCCAGTTAATTAACAGTGGTTTATCACCCTTGTAACTAGCCAAAATCACAATAAACAACACCACAAACAAACTAAGGATAATGATCGTTTGGGTGAAGATATTATCTTCAAAGATCTTTAGGGCCTTAGGCAGTTTTTTGTGCTCGGCACTATCGTTTTTGTCACCAAAGTACTTGCCCAATTTAGCAACCGTCATAATCCCTAACATCTGCTGGTGACCAATCGAAAAACCAGCATTATTGGTTACCTTATTGGTCACTAAATAGGTACCCGTAGAGGCCGTAGACCAGTAAACTCCCAAAAAGATACCACTAATAATCACTAGACCGGCTTGGGCACCGTAAGCAATGCCCGTACCCAACAACGGAATTTGGCGGAACAAGACAATATAGAACAGCGTAGTAACTACCGCTGCTTGTTGCAACATAACGTGTCCAGTAACCATAATCGAATTGGTGTTAGTTCAACGCTTTAACCCCACAAAGATAATGTTGACAATAAAGCCCACTAACAGCGAAAAGGCAATCAAGGACACATAGTCATTGTTCAAGAAACTGTTCGTAAAGAAATCGTTCGCTGATGCTAAGGAAAAGTAAGGATCCAATAGTACCACACCAGTACCCCCTAAACCCTTAATCGTGTCAAAAACGGGACGGGCGGTGGACACCAACACGCCACTACCAATGCTTAACAGGATAAAGCCAATTACGGTCTTTAGTGCCCCCACCACCGATTGCACCCCACCACGACCTAAAGCAAGGTAACCAATTAGGGTAATAACACCTAACAATAGCGCTGGTTGACGCAAAAAGTTATCGAGGTATACCCGCGTAATTAAAAAGATAAAGGCATCACCAAAGGCTTGGGCCGAAAACGGGGTTACTCCCTTACTAACTAAGTTCGGTAATCCTGCACGCAGTAATACTGTCAAGAGAATTACCACCAGGTTGATCAAAGCAAAAACGGATCAACCGATAATTAACTTACGTTTTTGTTGGAGCTTAATTTTGTTGAGCATTACTCTATTTTTAGTTGTTTTTTAAGTGCTAATACCCGTGGATGCACAGCCTTGGGATTAAACTTCCGCGGTGCATCAAAGGCCAGGATTTCAGCGGGATTTTCGACCAAGATGGTGTTAATGGCCGCATCGGAAACTCCCACTTCTTTAAGGAGCGGAATAAAGCGTTCAAACAGATATGCAAACCCAAAGCACTCCTTCCCTTTTTCTAAACCATAGTGTTTTGGTACAGTACCCGCCCCGCATCTAAGGCCAGGGTAATGTGTTTAACAAAGCCCAAGTCCACTAAGTATTTAATGTGTTTAGCCAATAAACAATCGGGGTAATACTTCACCCGATCGGGCCCATCAAAACACAGGGTCACACCCAGTTCCCGAATAATCTTGGCATAGTAATACTCATCGGGATTCTTGTTTAAGTGCGATAGTTAAATTTTCCGCGGATTAACGCCAAAATCAATCAGGTGTTGTACTGCTTCATATGCCATGGTACCGAGTTGGGTGTGCACGAGTACGGGAGCACCAGTAGTAATCGAAGTAATCGCGACTGCTTCCAAGGCCTTTAATTCCAACCGGTCAATCGCAGCATAGCCCGTACCAGCTTTAATAATGCCTGCCTTAGCCTTGCCCCGTTTAACCACGGGACCGGAATAGTTATACAAATCCATACCCTCTTCTATTTCAGCGACCAACATCGGCACAATTTCATTAACTGGCACTTGTGCTAACCAAGAACTTCCCTTGTCATAGAAAGCAGCCTTGTGAAAACCAGTGGCCATAATAATGTTCAGCTTGCCCTTTAGTTGTTCTGCAATCGCTACCATCCGTTTGACATCACGGCCCACATTCGGTGGATCCATGGTGACAATGCTCCGACCACCATGGTGGACAAATTACAAACACTCTTTAATAGCAGCTTCATTCGATAACATCACAAAATCAGGGTGCTCTTTGGCTTCCGGACCCCAATTCTTAATTAAATGATCGTGACAGTCACAAATACCTAAATCCTTAGGGTCAATGTCCCCTAAAACTGTTCTTACAAATCTTTTCATTTCTAAATGGACGTTAAATGGTTTAAGTGTATTGAAATAACCCTAATAATGTGTAAGCTACTGGATAATTTGCCATTAACAGTTGTAAAGCTGGCAACTATTTTTAGAATCAGCCATGGATCAAAAAATGATAAATGACCTCAAAGAACAGGTTTTTCAAACGAATTTATTACTACCCAAATATGGCTTAGTCATCCACACTTGGGGTAATGTTTCGATGATTGCCCCGAACCGTCAGTTCTTTGTCATTAAACCAAGTGGTGTGAGCTATGACAAGATGCGTGCCCAAGATATGGTAGTGGTCGATTTGGACAACAATGTTCTAGATACCAACGGCCTCAAACCATCAAGTGATACCCCAACCCATGCGTTAATGTACAAACATTGTCCAGATATTAAAGCTATTGTGCACACCCACTCTACTTTAGCGACATCGTTTGCTCAAGCCGATAAGCCGATTCCTTGTCTTGGTACTACCCATGCTGATAACTTCTTTGGACCAATCCCGTGTACTAGGGCTTTAAGTGACAGTGAAATTAATGGAGCTTATGAACATAATACTGGTTTAGTCATTCTGGAACACCTCAAAAACAACCAGGTTGATGTCAATGCTTGTGCGGCCATCTTAGTCAAAGAACACGGTTCGTTTGTTTGGTCCAATAAGAATGGCAAGGATGCAGTGGACCGGGCGTTAACTTTAGAACAAGTGGCCCAAATGGCCTTATATACCCAAATGATCAATCCCCACATGAAAGAAGCAAACCCAGCGTTACAACAAAAGCACTATAACCGCAAACATGGTAAAGATGCTTACTATGGACAAAATACTAAGCAAGAAGATTAAAGTGAACTGGTTGGGCGGGGTGTTTTGGTTATTACCTAACTTACTCGATCTGTTTTCCGCTAGCAAACGTAAAGCGAGTGTGCGACCCTACCAAAGCTTATTGGAACTGGTTCAAGAAAACTTCTTGAACCGGTATGACCTCGTGCACTTTAGCTTTAATGGTGACCATGACTTCTTTCACTTTAATGATTTACAAGCCATTAGGAGCTTTAACTTTACGATAGAAGAGGAACAACTAGGAGCAATGCAACCAGATGAGGTTTTACTGTTTGAACCTGTGGATCGTGTAACAGTGGAATTAGATCAAAAGGGTTTGAGTTTAATCCACAGTGGCAAAGCTTTTTGTGCTAGTGCAAACTACTTTAAGCACTGGCTAAAACGCGTACCACAGCAAGACAAAGTGACCCTAGTGTGGCGAAAATCAGGTTTTGAATTAAAGCAGTAGAATTACTCTAATACTATATAGCACTTCTCCCCCACGCTTTCCCGTTTCTCCACCGGGTTCAACCTCGCGGGGTCGGTGCTCGACCAGGTGTTGGACTATATTCCCTGGATTGGGAATGGGTACAGGTATGGGAATAACCACCGGGGTGTGGATGATATAACCGCGCCTCAAACCAGCGCGGGGTCGTCCAGCGGAACTAGTACGAACACAAGTGGTTCGCGTTCCTTTCTCCCGACGTTTTCGAATGTCGGCGTTGGCCTCAAAGCGAATGTCCAGGGCACCCTCGGGGGCAGTCAGACGACGACTACGGGGAACAATATTCCCAAATGACCCACCCTCGACCAAGCCAACCTCCAGCTCTGAACGGGGGCGGGGTGAAGGAATGATAAGACTACAAGTGGTTCAACCGGCAATGCCAATGACACCAAGTTCACGAGTGCTACGGGCAGTGGGAGTGGGCAGGGCAGTTCTTCAGGTACAAATACTTCCGCGGGGAATCCTGATGGGCTTCAGGCTGATAAAGTTGATCAAAACGGTCAGGTGAAAACAAGCGTTCAAGAAGCCACTTCAGGGGACAACTTGACGAATTACACCAACCTCCCCCCCAACCTCACCCCCACCGCTGATTGACCGAACGCGCTGTCATTCACCAACAAGAACAACGCGCAGCGCGCCCAGCTGTTCCTGCGCGGCCTGTTGGGCAGCATCCCGGTGTTGGTTAATAAGTCCGGCCAAGATGATAACAGTAAGTTTAAGGCGGAGGACCAAAAATGGTCCTACACCGACTTACAGTCGGACCAAACCAAACTGAACCTCCCCGCTTACGGTGAGGTGAATGGGTTGTTGAATCCGGCGTTGGTGGAAACCTATTTTGGGAACACGCGAGCGAGTGGTTCGGGGTCCAACACGACCAGTTCACCCGGTATCGGTTTTAAAATTCCCGAACAAAGTGGCACAAACACAACGTCGAAGGCTGTGCTGATCACCCCCGGGTTGGCTTGAACGCCGCAAGACGTTGGTAACCTCGTTGTCAGTGGCACCAGCTTCAGCTTCCAGCTCGGCGGGTGGTTAGTTACGTTCACGGACTTTATCAAACCCCGCGCTGGTTACCTCGGGCTCCAGTTAACGGGTCTCGATGTAAGTGAAGCGACCCAAAGGGAGTTAATTTGGGCCAAGCGGCCCTGAGCGGCCTTTCGTGGCAGTTGGGTCAACCGGCTGGGCCGCGTGGAGAGTGTGTGGGATTTCAAGGGGGTGTGGGCGGATCAAGCTCAGTTGGCCGCGCAAGCAGCTACAAGTAGTACCACCACCACCGCAACAGGGGCTACCTTACCGGAGCACCCGAATGCCCTCGCGTACCAAATTAGCTATACCGACAAGGATTTGTACAAGGCTTCCACTCAAGGTTCGGGTCAAACCAATTCCCAAAACAATTCGCCCTACCTCCATTTTATTAAACCTAAGAAAGTCGAAAGCACGACCCAACTCGACCAGGGCTTAAAAAACCTGTTGGACCCCAACCAGGTTCGCACCAAGCTGCGCCAAAGCTTTGGTACAGACCATTCCACCCAGCCCCAGCCCCAATCGCTCAAAACAACGACACCGGTATTTGGGAGGAGTAGTGGTAACCTCAGTAGTGTGTTTAGTGGTAGGGGTGCTGGAGGGGGTTCTTCAGGCTCAGGTCAATCTGGCGTGGATCTCTCCCCCGTTGAACGGGTGAGTGGTCACTAACTATTAATAGGAACTTTTCTTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACCAACTTTAAGCAATTTAGTGAACGAATACCCAAAAAGTATGAAATTAGTTAAATTAGCGAGGCTTCACCGCTTTTTAAAACAAGACCAATTGCAACTTGTTCCTTTGTTTAGTCCAAACTTTTTGCAAGTTTTGTTGGCGCTTCTCAATAAACTGGTCAATTTATTTAGTGAAAACACAACAATGAAAACAAATAAAAAATACGCCTTTGCGGCGTTGCTAATGCAAGCTCAAGCTAAGCTCCCAAATTCATTTAAATTCTTTCTTTCAAAGAAAGGAGGTATGGCTTAATGAAAGAGAAAATTCCGTTTTATAACGAAAAAGAATTCCATGACATGGTGAAAAAGACCAAAAAGGGCACTTTTTCAGGTTGATATATCATTGACAAAGATAACAACAGTGTGGAATTCTCTGGCAAATTCAACAGACAATTTAAACTTAACAAACCGGTTATACCAGTGAACACGGAATACGTAACGCGTAAAGAGTTTAACGAGTACAAAAACTCAAACGACCAACGACTTACAAAGATTGAAACCACTTTGGCCGCCCAAGGTGAACAAATCAACAAATTGACTCAAACTGTTGAAAAACAAGGCGAGCAAATCAATCAATTAGTTCAAGTTGTGCTTCTTCACGGTGAACAAATCAACAAATTAACTCAAACTGTTGAAAAACAGGGCGAGCAAATTAAAGAACTTCAAATCGAGCAAAAAGCTCAGGGCGAAACCTTAAAGCTAATCTTGCAAACGCTGCAAAAGATGAGCGATCGCTTGGACAAGATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAGTAAAAATGGACAAAATGGAAAAAAGAATAGACAAACTTGAGTCCAAATAACCAATGCTCAGAAATAAAAATACCAACAGTGAAACCTAAAGATGCGGCGCTGTTGGTATTTTTTCAATTCACATAAATAAAAAAGCCATCTGCCCAAGGTGAAAAAAACAGACGGCTTTTTGGCGATTTTGAAATTAACAGCAAGTTGTAACTTTTAGTGATTTGAATGAAAGGAATATATGACCTTTATTCTTTTAAAAAAAGAGATACATCAATTGTGCTGAAAGCCATTGCGTTAACATAAATAAATTAAGACATAAAAAATGAACACATTAATCAAATAAGTGTGGGAACAGAAAATTAGGCTTTCAACACAAAATTTTCGTTTTCCTGCCCCCACTAAATAAATTGAGTTGGGTTTTAAAAAGCGTCAAAAATTTTAATTTAGTTTTTGCTACTTTCCCCTCCCCCTCACGGGTGAAAACCCCGGCGCTTGGGCCTTAGTGCGCGATAACATTGCCAAGGGCATCACTGCCGGTAGTAACACCCAACAAACCACGTATGATCCCACCCGAACCGAGGCCACCTTGACCACCGCCACCACCTTTGCGTTACGCCGGTATGACCTCGCCGGGCGCGCCTTATACGACCTCGATTTTTCGAAGTTAAACCCACAAACGCCCACGCGCGACCAAACCGGGCAGATCACCTTTAACCCCTTTGGCGGCTTTGGGCTGAGTGGGGCTGCACCCCAACAGTGAAACGAGGTCAAGGATAAGGTCCCCGTCGAGGTGGCCCAAGACCCCTCCAATCCTTATCGGTTTGCCGTTTTACTCGTGCCGCGCAGCGTGGTGTACTATGAGCAGTTGCAGCGGGGGTTAGCGCTCCCTAACCAAGGGAGTTCGTCAGGCTCCGGTCAACAAAACACCACCATTGGCGCGTATGGGCTGAAGGTGAAAAACGCCGAGGCGGACACCGCGAAGAGCAATGAAAAACTCCAGGGCTATGAATCCAAGTCTTCCAATGGATCTTCAAGCACTTCCACCACCCAACGTGGGGGTTCGTCAAATGAAAACAAAGTCAAGGCGTTGCAGGTGGCGGTGAAAAAGAAATCCGGGAGTCAGGGCAACTCCGGTGACCAAGGCACCGAACAGGTGGAACTTGAATCTAATGATTTAGCCAACGCCCCGATTAAACGGGGCTCCAATAACAACCAGCAAGTCCAACTCAAGGCGGACGATTTTGGTACTGCCCCTTCCAGTTCGGGATCAGGCACCCAAGATGGCACCCCCACCCCCTGAACGCCGTGGTTAACGACTGAGCAAATTCACAACGACCCCGCCAAATTCGCCGCCTCGATCCTCATTCTGTACGATGCGCCTTATGCACGCAACCGTACCGCCATTGACCGCGTTGATCACTTGGATCCCAAGGTGATGACCGCGAACTATCCGCCCAGTTGAAGAACGCCCAAGTGAAACCACCACGGTTTGTGGGACTGAAAGGCGCGCGATGTTTTGCTCCAAACCACCGGGTTCTTCAACCCGCGCCGCCACCCCGAGTGGTTTGATGGCGGGCAGACGGTCGCGGATAACGAAAAGACCGGGTTTGATGTGGATAACTCCGAAAACACCAAGCAGGGCTTTCAAAAGGAAGCTGACTCCGACAAGTCGGCCCCGATCGCCCTCCCGTTTGAAGCGTACTTCGCCAACATTGGCAACCTCACCTGGTTCGAGCAAGCGCTTTTGGTGTTTGGGATTTGTTTGTCTTAATTAACTAACAAAATTAATAAATAGGTCTATAGGTTATATTAGTACACCCCCTACACGCCCTTTACGACACCGCTCAATGGGGGGCTGGATGTAGTGAAGGCGGCCAATTTACACCCCTCATACGAACTCGTGGACTGAAAGCGGGTGGAGGATACCAAGTTGGTGGCGCTGGTCCGTTCAGCGTTGGTCAAGGTGAAGTTTCAAGATAGTTCGAGCCAACAAGGCACTGAGAGTAATGGCCAAGACCAAAATGCCCTTAGTTTTGATACCACCAAGTCCCAACAAGCCTTACAGCAAGCTGGCGATCAATCAGGAACTTCATCTAATTCCACCCAAGAAGACTTCGCCAGCTACATCCTCATCTTTAAAGCCGCCCCGAAGGCCACGTGGGTGTTTGAACGGAAGATTAAGTTGGCGTTGCCCTACGTTAAGCAGGAAAGTCAGGGTTCCGACGATCAAGGTTCCAATGGTAAGGGCTCCCTCTACAAAACCCTCCAAGACTTCCTCGTCGAACAACCCGTGACCCCTTACACGCCCAATGCGGGGTTAGCCCGGGTGAATGGGGTTGCTCAGGACAAGGTGCATGGGTTGGAACAAACCACTCAATGAAACCAACAAGCCTCCCAAAAAAACCTCACCAACAACCCCGCCCCCAAGGCCGTCACCGGCTTTAAGCTCGACAAGGGCCGCGCGTACCGGAAGTTAAATGAAGCTTGACCGGTGTATGAACCCCTGGATTCGACGAAGGATGGCAAGGGGAAGGATAAAGATGGGTGAATCACTTCCGGGGCGAGTGAGGCCAAAGGAGATGCCCCGTTGGTGAGCTCAACCGAGAGTCAAATGGCTGCAGTCACAGACAGTCAGCAATCAGGTCATAACAGTGGATTGGTATCGCTTGCCCAGCGCTCCACTACTGTTGCTGTCCAAAAAAGTGACTCTTCTGGTTCTCAAGGCCAAGGCACAACCGACAACAAATTCCAAAAATATTTAAACACCGCCCAGGCCTTGCACCAGATGGGGGTGATAGTGCCATCTTTGGAAACGTGACCTGGTAAACCAAGTACTGGAATCGCTACCCGTGCTGGTGGTGGTGTTTCAGTCCAAGCAGCGACCCGGCAATCGTCTTCTACAAATGAAGATCTGCCGAATGTAATAACACAGTTATACCACACCTCCACGTCCCAACTCGCTTACTTAAATGGCCAGATCGTTGTGATGGGTTCAAACGCGGTACCGAGTTTGTGGTATTGGGTCGTGGATGAGCGAACCACGTCCGGGAGGGCGACTTGATGAGCCCACACCGAGTTGAACTGGGGCACCGACAAGCAGAAGCAGTTTGTGGAGAATCAGCTTGGTTTTAAAGATGACTCAAATTCTTCCTTGACAAACTTCAAGAGTCAAGGCTTAACTCAGCCAGCTTACCTCATCGCCGGCCTTGACGTTGTCCAAGACCACCTCGTCTTTGCGGCCTTCAAAGCGGGCGCGGTGGGGTATGATATGACGACTGATTCGAACGCTTCCACCAAAGACCAAGCGCTCGCCTGGTCGACCACGGCCGGGTTGGACAGTGCTGGGGGGTACAACAACCTGGTGGAAAACACGGCCGGACTGAACGGCCCGATTAACGACTTGTTTACCCTGCTCGACACCTTTGCTTATGTGACCCCGGTGAGTGGGATGAAAGGGGGGAGTCAGAATAACGAAGAAGTGCAAACCAAGTATCCCGTTAAGGATGATAGTAAGGCGAGTGCAAAGATTGCCTCCTTAATTAATGCTTCGCCCTTAAACAGTTATGGGGATGATGGCGATAAAAAAGCCATCTACCCAGAGGTAAGAGGCAGACAGCTCCAAAATATTTTTTGATGATTGGAAAACTAACGCAAGGTGTTAGTTTGATGTAGAGGCAAAACTATGAATTTACTAAATTTTATTTAAATCAGCGGGAGCAGGCTCGTTTTTTCAACACAAGTCACTCTGTCTGCTCCCACTAAATAAATTGAGACAGATGTTCAAAGTCGCCAAAGAGTTTGAATATTTTTGCAATTTGGGTTTGTAATCCCTTCTAACTATTAATAGGAACTTTTACTTTCAAAAAAGTTTTGAAGTTATTTTGGTGTCTTTACTAACTTTAAGCAATTTAGTTAGTGTAAGCACAAAAACCGTTATGTGTAATGTTTTTATTAATTTAGCAAAACAGCTGAACTTAGTTGGCAGCACTACTGCAAGTTGTAGTTTTCCCAACCTCTTTCAGAAAGGAGGTATTGCTTAATGAAAGAGAAAATTTCCGAAAAGGAATACAAAGCTTTAATCCGTAAAACTGGCAAAGAGCACTTTGATGGTGAAAAAGAAGAGTATGGTGATGGCACAGTTGGAGTTTGAACTTATGAATTGCGAAAATATAAACTTAAACCACCTGTGAAAGTTAAATACGTAACCCAAGAACAATTTCAGGAATATAAAGATTCCAACAATCAGCGACTCATAAAGATTGAAAATAAGGTTGATAAATTAGTTGAAATTGTTCAAATTCACGGCGAACAAATTAAAGCACAGGGAGAAACCCTGCAATTAATTTTGCAAACGCTGCAAAAGATGAGTGATCGCTTGGACAAGATGGAAAAAAGAATAGACAAACTTGAGTCAAAATAACTAAAGCCCAGAAATAAAAAGTCATCTTATCAAGGCGAAAGACGGACATCTAATGAATTGAAATCAATTACTTAATTAAATATTCCAAGTAAATAAAGAGATTAATATCTCTTGAGTAGCCGCTGGTATTATTAGCATAGCTACTCAAAAATTGTTTCACACTTTTTTGACTAACATCATCTTTGCCTTTTATTAATCAGCTAAGTGAAGCGCTAAACTCAATTCACTCTAAATTTCTTGTAATTTCTTTTCCATTAACTTTATTTTTTTCTTCTTTAGTAAGAAATTTTTCTGTAGTAAATTGTTTTTTTGGATCAGAATTAGATTTAATTTTAAGGAAAAGATCAAAATAATGCTTGTCACCTTTCTTAGCAGTGAAACCACCTGAAGAATAATCTCCAAACCAATTAAAGTTATAGCTCAACTTAGCTTCAATTGACAAACCCTTAAAACTTTGGTATGTCATTTGAAAATGATCAACCTTTCGCTCAAACTTAAAAGGTTCACTGGTTTTGGGATAAATTACATCTTCAAAATCAAGATCCGGAATACCTAATTCGCCCTTTTCAAAACTCTGTTTTCAGGCGTCTAACAACAATTTTGTTGTATCTTTCGGATTAACTTCGTAAGATTTCTTTAAAGAAGTTATTATGTTCTTTTTTCCTTCTGTAGTTTTAATTGCCTTGTAAACATCAATGTCTTTTTTGGTGGTTTCCTTGATAAGATTTCTGAGATCTGCTTGGATGCCAGTATAGGCGCTTAAAACTGTTCCACATAATAAAAATAGAGAACTAAAGGAAATCCTTGACAACAATCTCTTTTTGCGCATCAACAGTAAAAATAACTCTTTAATTTTTGTTTTTTTTTTTTTTTTGGAAAAGCAACTTTAAAAACGGAAAGCTAATTGAAATTTCGGTTTGAACATTAATTAAAAAACTGAAATTTTCAATCATTTATTAACAACTATCACTGATAAACATCTCGATCTGAGTGGTTCTCATAATTGCCAATTGTTGGTATTTTTATTCATTTTCAAGCTGTATAAAACTTTTTTGCAATTTTGTTGGCACTTCCAAAAGCTCATTTCAATTTATTTAGTGGAGGAGCAAAATGCAAATCTTTAATTTTTGAAATTGATCAGTAAAGAATCTGATCCCTGTTGGCAACCGAGTTGCAAGCGTTAGTTACTTTTTAAAAAATCAAAAGAAAGGAGGATTTGACCATTAAAAAAGTAAAGGTTACAGAGAAAGCTTCTGTTGAAAGAATGATAGAAGTATTAAGTGATTCTGAAATTTTGTCTTATTTATTAAGTAATAAGCACACTGCTGATGGAGTTAATGAAATTTATGTAACGATGAACATTAAAGACAAAGATTCAAACACTCAAAGGAAGCCAGATAAAATGTCTTTAGAGCTTGAATTGTTTTTAAATCAGTTTATTGAATTCTTAAAAGAGCAGAGAAAAAGCTAGCAGCATAAAAATACCAGCAGTTAATGCATAGCTGTTGGTATTTTTACTTAAGTTTTGACCATTTTGAATGGATAAAATTTCCTTGCAAATAGAAATTACAAACCAAAAACTATAACAAAATGAACTAAGCAATTTTCATCTTGGCTTTTTTCTTATTTAAAAGAGATTAAATTTTCATCTTAAAAGCTTTTATTTTTACCACACAGGATCTAATAAATTTCAAGAGTTAAAGAAGTACAAACTTTGTTAATATTTCTCCAAATTTCGGGTTTTTAGTGTTTATGAAATTTAAGTATGGTGCCATCTTTTTTAGTGGCTTTCTAGGTCTTTCTGCCATTTTGGCTGCCTGTGGTACAAAGGGTAAATTTGATCAAGTTGATGATGGCAAGATTAAATTGGCTTCCTCTTTAACTTCTAAAAGTGCATCAAAAGCCTTACAAGCAATTGTTAAAAAATATAACGAAGTTAAAAAACCTGGTGATTACCCTATTGAAATTACCCAAATCGCTGGTGGTTATGATGGCGGTCGTAGCGATTTACAAACCCGAGTCAACGTTAAAGACACCACTAACTTTTACAACTTAATCTTAAACTACCCTGATCTAGTTTCAACTTTAGGTCGTGTTGGTATGGAACTGCCGTTTGACAATGTAAAGGTTGACAAACTATCACCCCGTTTTTTAGATTTCAACAACCGCATTAGTGCAATTTCTAAACCAGGAATTTACGGTATTCCGGTTTCTTTATCCACCGAAGTTTTATCGATCAACGGACCGGTGTTGCACTATATTTTGAACAACGCTAAAAAGAAAGAAGGTACTTTAAACCAAAAAATGACAAGTTCTTCTGAAGGAAAAAACAGCAGTGGCACTTTAACAGTAGCAACTGATACTGAAACAAGTAGTTTATGGAAAAAGATAGAAGATTCTGCAAAAGCTAACGGTAAAAGCGATGAAAAAGGAAAAGGTAAGAAGAAAGATAATAAAAGTGCAACTTTTTCGCTTGTACAACTAAAACAAACTCAAGAAAAAACAGATGATTCCCAAGACACTAAAAATAGTGATGATCAAGTTAAAAAATCTTGAGGCGAATACCAAGAAGTAGATGGTGTTCTTAAAAATTTTGAATTTAAAGCGAGCATCTTCGAAAATTGACATGACTTACTTGATTTTTCTACTAGAGTAGCTAAATCTTTTAAGAAGATACACGAAAATAGCAACAAAAAAGGTAATGACATTCAGGGCATACTTGGAGTCGACAGTACGCCCAACTCATTGTTTACCTCTGTTTTTGCAGCTGGCGGCGGGGATTACAATAATTTCTTTTACAAGATTGAAAATGGTCGCGCTGACTTTAGTAACTTTAAGAATAAAGGGACTTCTTACCAAAATCTTCAAAAAGTATTTGGTGATTTCAAAGGCTTAATTGACAAAAATGGTATCTTTGTGAATAAGGGTGGATCTTACTCATCAAACTTCCAAAAGTTCCACCAATTAGCTTACAGCATTTCCTCTACTTCCGGTTTCTTCTATTCGTTTGCCGGTAAAAGTGCAAAGCGTTTAAATTTTGGTGATAGCTTTATTGAGTATCCGCGGTTTACTCAAGAAATTAAAGCACCATCAAAAAATGGAGAAAATGGCCAAACAAATGAAGGAAATAGTACCAATGGCGAACAAAATCTCTTAGGAACTTTCGAAGTCAAGGATGACAGTAAGCCTAAAGAAGAAGTTAAAAGTAATAAAAATAGTGGTAAAGAAAGTAGCCAAAATCAGGGTAAAAAATCTAACAATAACAAGACTATTTACCTTTATGAAACAAAAATTCCAGATGGAAAAACTGCAGGTGATAATGCTATCTTAATCAAAGATAAGAACGTTATCGAAAAGCTTAAAAGTGCAGCTAAAGAAGAAAATAAAGAACAAACTGCTGAAGCAACTAAAGCTGCAATTACAAGTAATAAAGCAAAAAGCACTAAAAAAGAAAGCAGTAAAGTTATCGGTTACACCACAACTGATAGTGTTCGCGAAGATGGCAAAAACATTTTCGCAATAGATAGAGTTAATGGTGAAAACTATGACCGTAAGATTATTGTAGGTGCTAAAGCAGAAACTTTAAACCAATCTAGCACCTTACAAAGTGAAGAAGCGATTGTTTTGCCTGCCCCTGGAAAGTATCTAAATGGTGATCCCAAGAAAGTGACAATTACCCAAGGACCTAACATCATTGGCATTCATGCTAACGAAAAGGAAAATGCAGAAACGCAAAAGTTCGTAGATTGATTCTTAAACAGTCCGCAAACTTGAGAAAAACAAAGTAGAGATAAGAAAGGATCAAGCGAAAAACAAACTGCTGCTGAGTTCTTTGCGGAATCCGCTTCTTACATTCTGCCCTTAAAAGAAATCTTTGACAAAAACGATACAAAAACAGAAAAAGGTAAGAACAGTAAAACGCAACAGAGAACTAATACCTATGCTGAAAAAGCTTTGGAACTCTTCAAACAAATTAGTCAAAATCAAATAGTAAGCTACAGTGATCCAAGTGACTTTAGGAGTGGAAAATTCCGTGATGCAATTGGTGCTACTTTCAATGCTGCAGTTAGTTCCAAAGCAGACTTCAATAAATTCGTCCAAAACTTTACCGCTACATTAGGTAGTGACATTTAATTCAAAAGCAGTTTTTCAAAATTCTTGATTTACCTTTAAAAGTATCAGCAACACAATAAAATTAACTGTTTGCAAGAGCTCTTTTAAAGCCTTTTAAAAGAAATTAAACTTTTATTGTTGTGCAAATTAGGACTTACAAAATCAAAGATATTTGCGACATTCAACGTGGTCGTGGTATCACTAAGGAATACATTAAAAATAACTCTGGAAAATATCCTGTTTATTCTGCCGCTACTACCAATAACGGTGAGTTAGGTTTTATTAATACTTACGATTTTGCTGGTGAATATGTTACCTGAACCACTAACGGCTATGCTGGCGTTGTTTTCTATCGTAATGGCAAATTTAGTGCATCACAAGACTGTGGTGTTTTAAAAGTAAGAAACAAGGAAATTAACGCTCAATTTTTAGCTTTTGCTTTAAGTTTAAAAACACCTCAATTTGTCCATAATTTGGGATCAAGACCTAAATTAAACCGAAATGTGGTAGCTGAAATCTCCTTAGATTTTCCACCTCTAGAAGTTCAAGAAAAAATTGCTCATTTTCTCAAATCTTTTAATGAATTAAGCAGCCAGTTAAAAGCTGAATTAATTAAACGACAAAAGCAATACGCATTTTACAGTGATTATCTACTCAATCCTAAACATTCACAAGGTGAAGAATACAAACTCTTTAAACTCAAAGATATAGCTAAAAAGATTTTAGTTGGCGGTGAAAAGCCAAGTGATTTCCAAAAAGAAAAGGATCAAGTTTATAAATACCCTATTCTTTCTAATAGTCGCAAAGCAGACGATTTTTTAGGCTACAGCAAAACGTTTAGAATTGCTGAAAAATCAATTACAGTTAGTGCAAGAGGCACAATTGGTGCTGTTTTTTACCGTGATTTTTCCTACTTACCCGCTGTTAGTCTAATTTGCTTTATTCCTAAGCCAGAATTTAATATTAAGTTTTTGTTTCATGCTTTAAAAGCGACTAAATTCCACAAACAAGGTTCTGGCACAGGACAGTTAACCATGGCACAGTTTAAAGAATATCAGGTTTATATTCCATCTTTAAAAAAACAACAAGAAATTGCTGCCACTTTGGATCCACTTTACTATATCTTTGCAAATTCTAATTGAGGAATATACAAAGAAATAGAATTGCGCAAAAAGCAAATGCAATACTATCAAGAACGTTTGTTTCAGTGAATAGAAAACCAAAAAGTTTAATTACTAGTTAATTTTATCCGTTTAGATTTAAGCGCTTAAAAGAACAAAGTGTAAATAGCTGGCTAGATCAACTACCGGTGGTTTGGTAGCTCCGGTGACTAGTTCTAAATCATGCAAGTTTTGTCCTCTCAATAAATTCATAAAGTTTGGATCGGCAAATGAAATCTGTTCTTTGACATACAATGTAAATAACAATTCATCCTTGTCATTAAACTGGAAGAGAGAACCAGGATAGTCTTTTTTAGCAACGGTGTAAACTGGAATTGATCCATCAACAATGAACTTTAAGTTCATTTCACCACTTCAAGAGCTTGGGAATTTAGTAAATAAAGCTGCAAAAGCATAGAAACCATACAATTGGTAACCACCGGTATATTTAATGTCCACTTCAAATCGATAATTAAAGGGATTAGCTTTATCTTGTTTTATTCCAACACCATCTATTCAATTAATGGTTGGACTTTCAATTTTATTTTGAACTTTTAATTCCTTAATCTTTAGATTGATGTTGGATTTGCTAGTAAGTTCACCGCGCAATCAATCGGACATGTAAATTCCATTTAAACCATCTTTAGGGAATTGGATTCCTTGACTACCAAAGGCCTTTTGCTTCGCATCGTTATTTTCTTTTTTAGCGTTGTAATACCAATCAATGGCAGTTCGAAATCCAGCAATTAAGAAACTGAAAGTTCTGTTTCTATCTGCTTCCAAGGAGATTTTTAAAGCCTCTGCTAACTGTTTTTTATCAGTGACATCTTTGTTCTTCTTACTTCAATATTGTTTGAATTCTGATGCAGCTGTTAGAGATTTGTTAAAAGCTTCTCTTTCTGCTTCGAGTTTTTTAACTTCCTCTGCTTTTGCTTTGGCCTCTGCTTCTTGAAGTTTACGCTCCTCTTCTAGTTTCCTTAACTTTTCAACGTGCTCAGCATGTGCTTTTTCTCTTTCTTGCTTGAAAGGATTGAGTACTTTTTCCCTAAATTTAGTTGCAACATCTGTTTGGTTATTTATTTCAATAACTGATTCTGTCAGGTTAACTTTAGTATCAAATAAAGAATCACCTAAAGCAGTTTGAGCAAGTGTAGTTAAATCATTTGTGCCGTTTGAAATTAGCTTATGCACTGATGGTTTTCAAAACAGTTGGGTATTTAAATCTTTACCAGATTGTCAGAAACTGTCTTGTAAATCTGTTAGATTGGCAAAATTAGTAGTGGATGCGTTGAGTGCAAACTGTAAATCTATCGTGCTACCATCACCTTCATAAATAGTTGCAAAATCCATTTTGATGGAAAAACTATAACAAACTTTGCCCTGAATGACTATGTCGTTTTTTCGCTCTAAATTTAAATCAAAGTTTTTGAGTGCAATCGACTTAAGGTTATATTGATCGAATAATGCAGCAATATTGTCAGCAATCTTTAAACCCTTTGGATGTAATTGCGGAAAGAAACCATCACCAAGTAAACCAAAAAGCTTTTGTGACACTTTAGGAATTTTAGTGCTGTCGGAAACAGGAAACACAGCATTAGCTGCATTTATTTCTGCTTGAAGTTGATTCATTAAATTAACCGTGTTTTGCACACTTTGTTTAAAACCACTAAGAAGGTTATAAACATCAATTAAACCTAATGTTTTTGGACTTAAAGCTTGTTTGACAAAGTCCTTTTCAAATTGTTCAAAGTTGTGCTTTCAATCCTTATTTTTCGCTATTACAGCTAACTCTTCCTCACTAAAAGTAGATGCGTTAGCAAGATTGACCAAGTAGCTGTCATTATGGGCTCTAGCTAAACTTTTATTGTGTAGTGCTAAAGTATTAATGTCAGCACTAGAACCTTTTTGAGTTAATGTAGGAAATAAAAATGCGCCTAAAGTTACAGTAGTGGTACAAAAAGCCAGCAAAAACGCTTTTGTTTTGAAGCGAATTTTTTTAGTTGCTTTCAATTTAGTTGAGAAATAAGCTAACTAAATTACTTATTAAACTAACGAAATCAATTAAGAATTAACTTTCAACCACAACAAAGTACTACAACAAACAGGGAAAATGTAATTTGTACGTAGTATTTTAGTTCTTTCTTGTGAAATATTGCAAAAGTTAGCTAAATCGAAATTAGCTCTTATCCGCTAAAATGAGAAAGTGGAGGTAGCTCGCTAAGTCTACTACTGGCACTTTAATGTCACCACCTCAAGGCTCTAATACATCGAGCTGTTGTTTCTTCAATAATCCTTTAAAGCGTTGATCCTGTACATTAATCCACTGAGTAATGTGTAGAGTAAATAACAGCTTGTCATCCCTAAACTGGAATAAGGTTCCAGGATAGTCCTTTTTGGAAGTAATTCAATCTGCTAATTGTCCGTCAACATTGAGCACTAATTCCATTTCACCCTTCCATTCTGGAGGAATTCCACCAAGTCCTCAATTTGCTAAAGTTCAGTAAATACCTTTAAGTCCATAATCACCGGTGTATTTCATTCTGATTTGGAAAGCATATTTAAACGGATAGGTTTGTCTAAACTCAATACCGTTCTTGGCTTCATTTCAGAAAATTTGTTTTGGTACACCCCAACCATTAATGTCATTGTGAATATCTTTTGTAGTTACCGATTGCAGGTTCAACTTTAAGTTAGCTCTGTGTTTTAGCTCTCAGCGTAACCAGTTAGGGATAAAGATTCCATCAACACCTAGTTTTGGTAATTCAATGCCAACATCACCAAAGGCTTTTTTCTTGGAATCTTGATCATCCTTTTTGGCGTTGTAGTACCAATCAACAGCATTTCTAAAAGCATCGGTAAGGAAAATAAAAGTTCTGTTTCTATCAGTCTTAAAGGCCAGTTTAAGTACTTCAATAAGTTGCGATTTAATTTCAACGTTTTTACCTTTTCCTTTTCAGTAATCTTTAAAACTTTTAACGTTATTGAATGAACTAAGAAAGCGTGTTTTTTCCCTAATTAACTCTTGAACTTTCTTTTCTTTACCTTTAAGTTCTTCTTCAAGCTGTTTACGTTCACGTTCTAAACGCGCTTGTTCTTCATCATGAATGCGTTTTGCTTCCTTACGTTGTTCAATGAATGGATTGAGGATGCGTTCCTCAAACGATTTGCGAGCTTTAGTTAAAACCGTTCCATCAATTACTGAATCAGTTAAGTTAACCTTAAGATCAAACAGGTTTTCACCAATCGCTGTTCTTGCAATTGTGGTTAAGTCATTGCCCTCATCAGTAATTAATTTATCTATTACTGGTTTTCAGAACAATTGGGTTGAGAGTGTTTGACCACTATTAGTAAAACTTTCACGTAATTCCTCTAAAGAGCTAAAGTTATTCGTTTGAGCTTGCAGAGCAAAATCAAGGTCAATTTTGGTATTTTTGCCAACATAAACGGTGTGAAATTTCATTTCGATGGCAAAACTATAGCGCACCTTGTTCTTTAAAACAATGTCGTTTTGTTTGAGCAACTGCAAGTTAAACGCTTTTAACTTAATTGAATTTAAGTTGTAGTCATTAAACAAAGAAGCAACCGTATCACTAATATTTAAACCACGTGGTGTTAATCTTGGGAAGTAATCTTCACCAAAAAAACTAAAGAAACCTTCTGGTACTTTAGGGATCGTGTTTTTGTCCTCTACGGGAAAAATCTCGTTAGCCGTATCCGCCCGTTTTTGTAAGTTGTTTGCTAAATTTACTAAAGCTTGGGCTTTTTGTTGAAACCCACTAAAGAGGTTATAAACATCAATGAGAGTAAAAGCTTGGGGATCAATAAGTTGGTCAGCAAACTTATTTTGGAACTCGCTAAAGTTTTGTTCTCAATCAGTGCGACTTGTAATCGTTTCGAACTCGGTTTGACTAAAAGACGAGTTGCGCTGCAATAAGTAACTCTTGTTGTGTCGCTTATCAAAGCTACTGCTATTTAAATTAACAGCATGTGGATCATTAGCTGAACCGTTCTGTGATAAGGTGGGTACTAGAAAAACGCTAAAAGTTAGCGTTGCTGAAAATAAAGACAGCAAAAACGCTTGCGTCTTGAAGTGAAAGTTTTTCGAAGATTTCAAAGATTCTTGTCAGTGTGACTAAAAGAAATAAATTAACTTACAGGTTACGAAATGCAGACAAAATAAAAGAAGCAACGGAAAATTTAAATTCAGAAAATTCTAGTTTTTTTTTTTTTTTTAGTTTGAACTCAAAACGGGTTGGTGAAAGAAACTGCAATAAGCTGTTGTAAATAACTTTAACTAAGTTAAAAACTGTTTCAAACTAACCAAGCTTACTTTTAGTGTGCCTTATTGGAAATTAATGTTGGCTTAATTATTTATCTGCCAAAATTAAGAAGTGGAGGTAGCTCGCTAAATCCACAACTGGTACTTTAATTTCACCACCTCAAGGTTCAATCACATCCAATTGCTGTTTCTTTAGCAGGTTCATGAAACGTTGATCCTTAACGGTAATACGTTGCACTACGTGTAGGGTAAACAACAGTTTGTCATTTTGAAATTGGAATAACGAACCAGGGTAATCTTGCTTTTGGGTAATCCAGTTAGCTAGCTTACCATCAATTTGGAACTTCAATTCCATGTCACCACTCCATTCTGGCGGAATGCCACCAAGACCCCAGTTAGCCAAAGTTCAGTAAATCCCTTTTAACCCATAGCTACCTTTGTAATTCATGGTTATTTCAAAACCATAAGTTCAAGGAGTGGTTGCTCTGTAATTAATGCCGTTGTTGTGGTCGTTCCAATTAATCGAAACAGGCACACCTCAACCATAAACCTCAAAGTTGTTTTCCCTTACTTTGACATTTTGTAATTGAAGTTGAATATTAGCTCTGTTGATTAATTCCCAACGTAACCAGTTAGGCATAAAGATGCCGTTAAGACCTGATTGAGCAAAGGAAATGCCGCCGCTACCAAATGCTGTACGTTTTGCTTCTTCATCGTGTTTTTTATCGTTGTAATACCAATTAATAGCATTCCTAAAGGCATTGGTTAACAGTTCGAAAGTAGGGTTCTGCTTGGCTTGGAAGGAAAGCTTTAAGGCTTCGATTAGTTGCTTCTTGTCTGCTACATTTTTACTGCGCTTAGCTCAGAACTCCTTAAAACTCTTAACGTCTCTAGTAGATTGGTAAAAGGCTCAATGTTCTTCACGAATACGACGAGCTTCATCTTCCTTGGCTTTCAATTCAGCTGCAATCCGTTTTCGTTCCGCCTCTAAGCGTCTTGCTTCTTCATCATGAATGCGCTTAGCTTCAACTCGTTCAGCGTGGAACGGATCTAAGATCTTGTCCTCAAATGATTTTTGCGCTTGCTGTAAAACGCTTTGATCAATTATTGAATCAGTTAAGTTAACCTTGAGGTTAAACAAACTTTCACCAATTGCTGTTTTAGCAATGTTGGTAAGATCATTGCCACCATCTGTGGTTAATTGTTCTACCACTGGCTTTCAGAATAGATGGGTTGAAAGAGTGTTGCCATTGTTTTCAAAACTATCACGTAGTTCTTGTAAGCTAGTGAAGTTTATTGTTTGGGCTTGTAAACTAAACTTTAAAATAGTGGTGTTCTTTCCAGCGTAAACCGTGTCAAAGCCCATTTGAAGAGCGAAGTTGTAACGCACCTTATTCTTTAAAACAATGTCGTTTTGGCGCAACAGTTTTAGGTTAAAACCAGTAAAGTCAATTGAATTTAAGTTGTAGTCATTAAACAAGGAAGCCACATTATCGGCAATGTTCAAACCACGGGGAGTTAACCGCGGAAAGTAATCTTCCTGAAAGAAACTAAAGAAGTTTTCCGGCACTTTAGGGACAGTAAGCATTGTCCTCTACGGGAAAGATTTCGTTTGCCGCATTAACTCGTTTTTGCAAGTCATTGGCTACATTAACTAAACTGCGTGCTGTTTGTTCAAAACCACTAAAGAGGTTGTAAACATCTGTAAGTGTAAAATGCTCTGGGTCAATTGCTCGTTGGGCAAACCGTTTTTGGAATTCACCAAAGTTTTGCGCTCAGTTAGTTTGCTTGGAAAGGTTTTCATACTCAGCGTTACTTAAAGCAGTTTTGCGCTTCAATAAGAATTTCTTACTGTTATGCTTTTCAAAGCTACTGCTGTTTAAACTTACAGTTTGTGGATCAGTAGCTGAGCCATTTTGGGAAAAAGTGGGTACCACAAAAACACTAAAAGTTAGTGTTGCTGAAAGGAGCGAAAGCAAAAATGATTGCGTCTTGAAGTGGAAACTTTTAGTTGGTTTCAATGTAAAGGTGTCAGTGTGACAGAAAAAGATTGGTTTACTTTTAAAAAGTGAAAACTAAGACGACAGGGAAAATTTAAATTTAATAAATTTTAGTTTTTTCACCGAACAAAAAACGGTTTCATCGGAAAATGAAGCATAAACGGCTCAAACAACTTTAAGAATGTTTAATGATGCCTTGATTACTGTTGATTTAACTTCAACAGTTGGTAGTGTAAATAACTCGCTAAATCCACTACTGGCGGCTTTGTGGACTCGGTACGTAGTTCTGAGTTATGGAGGTTTTGTGACTTAATTAATTCCATGAATTCTGGCTCTTGCACCGTAATTTTTTCTCACACATGGAGCTTAAATAACAGCTGTTGCTTTTGATCATCAAACTGGAACAACGAACCTGGGTAATCGGGCTTTTGGACAATCTCTCAACTTAAATCACCATCAACAATAAATTTTAAATTCAATTTACCCTTTCACTCTCCGGGAATACCAGAAAACTTGGCAATAATGGCTTCTAATCAACTTAATTTGTAATTACCTTGATATTCAATGTCAATCTCAAAGCGGAAGTTGTAAGGTTTGTCTTGATTTCGCTTAATACCGATATCTTTGTTCCATTTAATGGTAGGACTTATCTTTGAATCAGTTACCTTTAAGCTCTTTATGTCTAAGTTAATCTTGGCCTTTTCCCCTAGCTGTTTACGCAACCATTCCGACATAAAGATGCCCTTTAAACCAGATTGGGGAAAGGCGATACCTTGTGTACCAAAAGCAGTTTTAGCAGCCTCACTTTGATCTTCTTGGTCATTTGGGTAATAATTGCTAATACCGTCCTGGAAGTTCTTAATCAAAAAGGCAAAGGTCTTATTTTGTTCTTTGGCAAAGGAAAGTTTTAAAGCTTCTAACAGCTGCTTTTTATCGGTAACATCTTTCTTTTGCTTTTGCCAGTATTCTTTAAAGGCTTGCACTGACTCAAGCGATTTGCTTACTTCTGCTAGCTATTCAGCTTCCTTTTGTTTTTGTTTTAATTCTTCGAGCTGTTTTTGGCGTTGCTCCTCTAAGACTTTTTGGTGTGCTTGGTGTTCCGCTAGTGCCTTTTGCCGTGCTTCAATGAACGGTTTTAAAACAGTTGCTTCAAACCTTGATTCAGCCTCTTTTAGCACGGTTTGATCCAAAACTGACTTGGTTAAATTAGCTTGGGTGGTAAATAAATTATCACCAACAGCTGCTTTTGCTACAGTTGTTAAATCATTGTTATTACCCAACACTAACCGCTTGACCGTTGGCTTTCAAAAAAGTTGTGTAGTTAAGTTCTGGCCACGTAAAACCAAGCTGTCTTGCAATTCCTGTAAGTTAGCAAAATTAGTGGTCATTGCCTTAACTGAAAAAGCTAAGTTGGTTGTACTGTTTACACCCGTATAAACTGTTTCAAAATCTCATTGGATTACGAAGCTGTAACGCATCTTGTAGCGCTTCACAATGTCCGGTTGTTTTTCTAGTTTCAGATCAAACTGCTTTAACGCAATGCTTTGAAGTTTGTATTGTTGCAAAACTGTCGTAATATTCTCAGTCACATTTAAACCCTTAGGGGTTAATTGAGGAAAGAAATCACTACCCAAAATGGTAAACAGCTGTTCGGGTACTTGGGGCAATTGAAATGCCTCGTGCACCGGAAAAACGGCATTTGCTAGTAATACCTTTGCATTTAAGTCATTAATTAACTTAACGGTATCACGCGTACTTTGTTCCAATCCACTCAACAGGTTGTAAACATCAATCACACCCAACTGTTGTGGGTTAAGCGAATTGTCCTTAAACTTAGTTTGCAACTGTTCAAAGTTAGCCTTTCAGTTAGTGTGTTTGGTGGTGTAATCCAGGTCAGCTTGACTAATACCAGAATTAAATTGCAACTGGTAGCGGTTGCTTTGGTTTTGTTCAAAGCGTTGGTTGCTTAAGGTAAAGTCACTAACTGACTGGTTTGTTTTTTCAGTTAAAGTCGGTCAAACAACGATAGCTAGAGTAGTTGTTAAACCAGCTAAGCTTAACGATAACGTTTTGAGAAGTAAACGTTTCTTTTTAATTAATTTCAAGTTTGGTTAAAAACGGTTTAACCAAATAGTTAAATTACTAACCGAAATGAATTAAAGCAACTACAACAACAGAAAAATTTAATGCGTTAATAATTGTAGTTTTTGTACTTTACTGTGTTGACAATCAACTGTTAAAAACAAATAATACTGTTAGCTTTTATCGATTTGTTGCACCAGCCAGTCTTTGATTTCGCCATAATAACTAGAGATACTGCGCACTAGTGCTTGACCCACTTTTAAACTTTTAATTTCACTAGCACAGGTAATATCAACCCCATACAGGTTAGCCACTTCTTCTGCTTCCATCATGTCAATTAAGCGGTGGGCAATTAGGGTACTGGTGTTGTTAATAATCTGTGATTTAAAGTTATCACCACCAATATTGCGCAGGTTAGCAAATGATTGATAGGACAGGATAACTACAGTATTGAACGAGCGGACTTGCTCCAATACCACCCCCACATAGTGGCTCCCAAAATTACCAAATTCATCAGCAATAAAAAAGGTCGAGTTATCACTCGTTTCTTCCAAACTCGTAATTGTTTGGCCAAAATCCAAAAAGATTAACTTGCCCAAGTACTTGGCATTAATCATGTCGTACATGCTGCTAATGGAAAACAAAACAGCATCTTGGCAGTTTGATAATATCTATCAATCCCACACCATTAGAATCGATTGAACCAATTTCCGCTAGTTCCCTGAGGTTTTCTCTTAAATTTTCAAAGTCCTTTGTGTCAATGGTTTGAACATGCTCCGTATAGTATTCATCAATTTTTTCTACAAAAATTTGATTGGAAGGTTGAAAGCGACCTTTTTCTTTCATTTCTAAAATTTCTTTTCGGCGTTTTTCTTTGTGAATTTTTTTAAACAAACTGGGTTCAAATTTAGCAAAAATATCAAAGTCACGTAGTAATACTTCATTTCTCCTTTGCTACAGTAACGCGCTAAATTCTTAAAACTCAAATTGTGGGGACCTTCCTTTGCAATTACTAAAGGTACTAAAACTTGCAAAATGGCCTTCTTATTACGTCAATAATAAGCACTGTCCTTGGTGTTTTCCACCATATCGTTATAACCAAACATTTCCAAGATTAAAGTAATGATTTGTTTGGGTGTTTTTTACTTAAACGGGTTGTACTTAATCGTGGCAATTCTGTCATCTAAGCTCCAAATTTTGGCACTAGTATTTTTGAGTGCCACTAACTTAACCGTATCAAAACCGTCTTCGGGACTTAGTTGTTTGGCGCTCGTTTGGTAGTGTTCAAACTGGGTAATTAATTCCTTATCACCCTTACCATTCACAAAAATTAAGGGATGGTCGTTGTCAATTGCATACTTCAAAAAGTGCAGCAAGATATTGGACTTACCAAAACCACTCACACCTAGTACCGAGACGTGCTGGGGGAGCTTGTCCAGTTCCAAAAAACACTCCTCGTTTAAAAAGATTTTCTTACCAGTCTGGTTATTGATGTCCTGTTCCATTACTAAAAAATAATTTGTACTGCAACTTCAAAGTTAACTTATTCTAATTTTGCTAGATCATTGGTTTCATTCACAGTTTCCCAACTTAGTTTTCACTGTTCAAACAATTAAAAAAGCACCAGCTAGTGCCAGTGCTTCTTTAATCGATAGTTTAGTGGTTTTTAATACTCCTCTTGAACCGTTTTGGCACCGAGGTAGGCCAATTCTTCATCGGTTAAAAACCCAGTACCAGCCGGGATCTTACCCCCAATGATCACGTTTTCTTTCAACCCTAAGAGGTAGTCAACTTGGGTTTTTACCGCAGCATCGGTCAAGATCTTCTTGGTGTCTTGGAAGGATGCGGCACTCAAGAAGGAGTTCGACTTGCTGGCCGCCTCATCGATCCCAAACACCTGGTTAATGGCAATCACTGGCATCTTCCCCGACAGCAACAAGCTCTTGTTGAGTTCATTGAGGTGGTGACTGTGTACCAGTTGACCCACAAACAGGTTACTGCTGCCAGCATCGGTTACTTGGAGTAAGCTCGTCAACTGGCGGATAATAATTTCGACATACTTATCAGCAATCTCAATCCCCTGAATACGGTACACCTTTTGGATTTCGACAATCATGTACTGGCGTACCCGTTGGATCCCAGCAACCCGTAATAACTGGCGAATGTCAATCGAACCTTCGGTAATCTTCGAACCTAGTTCTACTGCATCACCGACCTTGACACGCAGTTGCGCACTAAACGGAATCGAGTAAGTGCGTTCATCGACACTAGATTCAATCAACACTTCCTGCGCGTTTTGGGTGGTAGTAATCGATTTAACTACCCCCTTAACTTCAGAAATGACACAGCGCTCGTAATCCTTAGGGGCGACTACTTCAAAGATTTGCTTTAAACGTTCAAACCCTTGGGCCAAGTTGTTTTCGGTCGAAACCCCCCCGGTGTGGAAGGTACGCATCGTTAACTGGGTTCCTGGTTCACCGATCGATTGCGCTGCAATTACACCCACAGCGGTACCCAATTCCACCAGTTTGCCGGTGGACAAATCGACCCCGAAACAGTACTGACACACGCCGTTTTCGCGTTCACAGTAAATCACAGAGCGCACTAACACTTGTTTAATCGAAGTGGCACAAATTTGCTTGGCTAACTCTTGGGTAATTAGGCTGTTAGCAGGGACAATTTCCGCCTTGGTTTCGGGATCTAAAATTGGTCCAATCGTGTAACGGTTGACAATGCGGTCAAACAGTGATTCCACCAACGAACGGGTTTTGGTTTCCACAATTGCTTCGACCACAATTCCCTTGCGTGTACCACATTCATCGTGGTTAATAATCAATTCGTGGGTTGCATCCACCAGTTTACGGGTCATGTAACCAGACTTAGCCGTTTTCATGGCGGTATCGGTCATCCCCTTACGCGCCCCATAAGATGAGTTGAAGTACTCGTTAATCGTTAAACCTTCCAGGAAGGAGTGCTTAATAGGAACCTCAATCGTGTCCTTAATAATTTTGGACTGGTTGTTGCGTTCATAGTTAAAGCTCTTACTCATTAACCCCCGCATCCCAAACAGCTGGGTAAAGTTGGAGATATTACCCCGCGCCCCGGAATCGGCCATGACAACAATCGAGTTATCACGGTACTCTTCACGTTTAATTAAGTCCTGGATCTCACTCGACACCTTTTCCTTCACACCGTTTCACAGTTTCACCACCCGTTTATACCGTTCATCATCGGTCAGTAAACCCTTGTTGTAAAACTGCTTGTACTTCAAGACCTGTTGGTCCGCTTCATCAAAGTACTGCTGTTTGTTGGAGTACTTCGGAATATCAAACACCGATACAGTGGTGGAAGAGTGGGTCGAGTACTTAAAGCCTAAGGCCTTAATGTTGTCCAAGATCCGTGGTAGATCCTTAAATTCAACCACCGTGTAAAGTAAGTCAATTAACTTGGAGATGACCTTCTTACCAAAGGCAGGGAGCACTTGGTGTGCGGCAATCGCAGCACGCACATCACCACTTGGTGGTACTATGAACTTTTGCGGACACGCATGTTCATCAAATTCCCCTTCATTAATGTAAGGGATGTTGTCCCCTAACACATCGTTAAAGATAATCTTACCAACCGTAGTGATTAGAGTACCTTGAGCTTCAAAGTGCTTGTTTGGAAAGGCTTTGGTGCTAATGCCGACAATGGCGTGGAGGTGCACCTTGCCCGCTTCGTATGCCGCGCGGGCTTCATGCACGGTACCAAAGATGATGCCTTCACCCGTTTGACCCTTACGCTCAGTAGTGAGGTAATAGTTACCTAACACCATGTCCTGGGTTGGGGTGACAATTGGCCGGCCATCTTTTAGTCCCAAGATGTGCTTGGAAGCTAACAGAATAGCACGCGCTTCGTTAACGGCATTTTCGCTCAACGGAACGTGCACCGCCATCTGGTCACCGTCAAAGTCAGCGTTAAAAGCAGTGGTCACTAACGGGTGTAGGCGAATCGCTTTCCCATCGACAATCCGCGGTTCAAAGGCCTGTATTCCCAAACGGTGCAAAGTAGGTGCCCGGTTTAATAACACCGGACGGTCCTTAATGACATCATACACAATCCCCCAGATCAGGTCGTCCTGATTTTTAATCATGTCCTCCGCTTGCCGAATCGAGGACGCAATTGGTCTAATTTCGTTACCGTTGCTGTCAAACTTATTAATTAACCCGTGAATAATAAATGGTTTGAACAGCTTGAGGATCATTAATGCTGGAATCCCGACCTCATACATCTTTAGTTCTGGACCCACCACAATTACACTCCGACCAGAATAGTCCACCCGTTTACCTAAAAGGTTGTGACGGAACAACCCTTGTTTCCCTTTTAAGCGGTCGGTGAGTGACTTCAAGGAACGTTTGTCTTTGGATAACGCTGGCTTGTGGCGCGACGAGTTGTCAAACAAGGCATCAACTGATTCTTGTAACAAGCGCTTTTCGTTGTTAACCACAATGGCAGGTACGGTACCATCTTCCAAGATGCGGCGTAAACGGTCGTTACGGATAATTACCCTTCTGTAGAAGTTATTAATGTCACTGGTGGTAAATTTAGCACCATCTAACTGGATAATCGGCCGGATGTCTGGCGGGATCACTGGCACCGTGTGCAGGATCATGTTTTTCGGGTGCAGCTTCGAATTTCTAAACCAGCTAATTGTTTCCAATTGACGCAAGATCTTTTTAACCTTAGCATCCTCAACACTATCCTTCTTAGCCTTCCGTAACGCATCATTGAGCTTACTAAACTCATAGTTAAGGTCAATCTTGTTGAGCAATTCTAAGATGGCTTCAGCCCCGATACCAACGCGGAAGCCAGTGTACTTCTTAATGTAGTTAAAGGCATCATTTAAGGAGAACGGTAACGAACTGTTCTTTAAGCTTTCATAAAAGATCTTCCCCTTGCGGTAGTCTTCGCTACTCCTGTTCTTAATCAGGTTCCGGAAGATGTAACCGATCACCCGGCGCATTTTTTGCCGCGTGGTTAAAGAACCCTTGCCTGCTAAGTCCAACACTTCCTTAAACTTAAACGGCATGATCTTGGGATCTTTGATCTTCCCAGTATCTAATACTATGTAGTTAACAAAGTACAAGACCTGTTCCACTTCCTTATAAGAGATGTTTAACACTAAGGAGATCTTGGAAGGGGAAGGCAGTTCCTTCGACATCCAGATGTGCGCTACCGGACTGACTAGGGCAATGTGGCCCATACGTTCCCGGCGCACAATTGATTCGGTTACCCAAACACCACAGCGATCACAGCGCACGCCACGGTACTTAATCTTCTTAAACTTCCCACAAGCACACTCATAGTCCTTCACTGGACCAAAAATGGCTTCATCAAACAAACCACCAGGTTCGGGTTTTAAGGACTTATAGTTAATCGTTTCCGCCTTGGTAACTTCCCCTTCGGATCAGTTTAGGATCGTGTCGTTGGAAGCAATCGAAAGCTTAATTGCCTTAATGTTCTTGTACAGCTTGTTGTTCTTTTTATTACGCTTTGTCATTAATTAATAACCCTCCGTGTCAAATTCAAAGTCATTAAAGAGATCGTCCTGTTCTCCATCAGCTTGGAGAATTGAAACGTTGTTGGAATCTTGTTGGGTGTTGTCATCGTAAATAAAGGACACACTCAACGCTAACCCTTGTAGTTCCTTCGTTAACAGTTTAAACGATTCGGGGATACCTGGTTCGGGGAAGGCAGCGCCCTTAACGATCGCGGCATAGGCCTTATTCCGGCCCTGCACATCATCGGACTTAATGGTAAGTAATTCCTGAAGGTTGTAAGCAGCGCCGTAAGCTTCCAAGGCTCACACTTCCATTTCCCCAAAGCGTTGGCCCCCATTTTGTGATTTACCACCCAATGGTTGTTGGGTAATCTTGGAGTAAGGACCGACAGCGCGAGCATGGATCTTGTCATCAACCATGTGGTTTAACTTCATCATGTACATGATTCCCAAGGAAATTGGTTTTTCAAATGGTAAACCAGTGCGACCATCAATCAGCTTGAACTTCCCGTGCGTTTCCGCTGGTTTCAATCCTGCTTCATCCATGATGTCTTGCAAGTCTTGGAAGTTCACCCCTTGGAAGATCGGGGTCGCTACTTTAAAGTGGAGGTCATCAAAGCTCATGCCGATTTCCTTGAGCACTAAAGCAATGTCACCGTTATTGAGTTGGGCCAAGGCTGCCTGCTCGTCTTTAAGACCGCGGTTCGTAATTTGGGCTTTTAAACCAGTAATTAAGCGGTCAAGTTTGTACTGTGGCTGGTTAATTTCGGTTGAAAGTGCTTGAGCCTTCTTGTCATCAAAACAAGCACTAATTAACATTCGCTTGGCAAGGTTATGTGCTGCATAACCCAAGTGCATTTCAAAGATTTGGCCAATATTCATCCGACTCGGTACCCCCAGTGGGTTTAAGAGGATGTCGACTGGAGTACCATCTTCTAAGTGGGGCATGTCCGCTACTGGGACAACCTTAGAGATAACCCCTTTGTTACCATGGCGCCCTGCCAACTTATCTCCGATTTGGATCTTGCGTTTTTGCACCACATAGACCTTAATCATTTCGATGACACCATCATTAAGTTCATTACCGTTGGCAATGCTAAAACGCTTGACACAGGACACAATCCCATCACCACCATGAGGGAGCTTTAAGGAAGAGTCACGGACGTTTTGCACACTTTCAGGGAAAATGGCCTTAAACAGCTTTTCTTCAGGTGATACTTCAACCTGTCCCTTAGGGGATACCTTACCGACAAGGACATCACCTTCCTTAACTTCAGCACCGACCATGATAATGCCGTTTTCATCTAAGTAACGCTTGTTAGCATCACTAACGTTGGGGATATCACGGGTAATTTGTTCATCACCGTTTTTGGTCGATAAACACTGGGCTACGTATTCGTTAATCGTTAAGGAGGTTAGAACATCATCCTTCACTAAGCGCTCGGAAATCACTATCGCATCCTCATAGTTGTACCCGTTTCAGGTCGTAAAGGCGACAAGGACATTTTGCCCTAAAGACAGCTCACTCTTGTTAACTGCAGGACCATCAGCAATCACTTCGTCCTTAGTTACCCGTTGCCCCTTTTCTACTAAGGGAACGTGGTTGTAGCAGGTATTTTGGTTGGAACGTTCAAACTTAATTAGATCCAAAGTGTGCTGTTCACCACTGTCACTTTCAATCGTGATCTTACTGTTGTCTACATACTTTACCGTACCTGAGCAAGGTGCTAACATCGTCAAACCGGAGTCGCGAGCAATCTTGTACTCTTGACCTGTACCCACAACGGGAGCGTAAGGCTTAATTAAAGGATAGGCTTGGCGTTGCATGTTGGTACCCATCAATGCCCGCGCTGAGTCATCGTTTTCCAAGAAGGGAATTAAGGACGAACCGATGGACACTACCTGATGAGGGGCCACGTCGATGTAGTCAATCTTCGAGGGATCATACAACCCTTGCATCGAGCGGTAACGACCAATAATTTCCTTATCTAAGATCTTGTTGTTTTCGTCAATGTTGACCAACGAAGAGATTTCGGCAATAATGTGCTCGTCTTCCCTTAATGCCGTGAGGTATTCAACTTCATCGGTAATGACACCATTTTTAATCTTGCGGTAAGGCGCCATTAGGAATCCGTTTTCGTCAATCTTGGCAAAGGAAGCCAACGACATGATTAAACCAATGTTCATCCCTTCCGGTGTTTCAATTGGACAAATCCGACCATATTGGGAATAGTGCACATCCCGGATATCCAAGTTCGGGTCTTCGCGGGAGATACCCCCTGGACCCATGGCGGAAATCCGGCGCTTATTACTTAATTCTGAAAGTGGATTTTGGTGATCTAAGAACTGGGTCAACTGGTGAGTGTTGAAGAAGTCACGAATGACAATTTGAATAGGTTTGGAATTAATTAAGGATTTAATGGTCAATTCCTTCTTTTCCGCTTGTTCAATTACTTGACCCTCTTCGTTAATTTGTTGACCACGGTTCACTCCATCAGCAATCGTGAGCTTTTCCTTTAAGAAGCGCTCCATGCGGGTGATTCCACTTTCCAAACGGGAACTAATTAACTCGTTAATTAACTTGACGCGTTTGTTACCTAAGTGATCAATGTCATCGTACTTACCGATGGCCGAAGGGATGTTAATCACATAACTAATCGAGGCAATAAAGTCACTTAAAGTAATTGCCTTATTGAGATCGTTATCGTTGTGGATCCCCACCACTGGCACGGATACATCGAAGTTGTCGTTGTTCACATAAACATCAAGTGATTCATACAACAACGATTCAGGGGAAAGGTTAACAGCACTACCATCAGTGGTTAACTTAATCTTTTGGACAAAGTCAATTTGGTTGTTTTGGGCAGCTTGCTTAATCTTGTCGATTTCTTCTTTAACTAATAAGGTGCCCTTCTTTAATAAGAGTTCCCCGTTTTTTAAATGAAGGTCACAAGCAAGTGTTTTTTGGTAAATCCGCTCGGTAATTCTTAACTTACGTGATACCTTATAACGACCAGCAGCGGAGAGGTTATACCTTCTTTGACGGAAGAAACGTGAACACAGTAAAGCGTGGTAACTGTTTTCCGGACATTCATCGGTGTTTTCTAAACCACGTAGTGAAATGGACAATTCATGAACAATGTGTTTAGCAGCCCGTTCGGTGATGATTTTATCCATCAACTCATCCATGTCCGCTTCTAACTGACCTACTTTAGGGTCATTTGGTTTAGCTTCACTGAGCGCTTTGTGTTCCTTTTCTAAAAGGACATAGTCACTCACTAAGCTGCGGATCTTTTGGTCAGAGGCAATCCCCTTACGGCGGAGATCAGTTTTGCCATCGCGGAATTCCCTTAATAAGTTTTTAATTTCAGGGTCAACGTTGTTTAAGAAATCCTTAGCGTTGTAGACTTCCGCTTCTAAGGAACGGGTAATAAACTCGTTGTTTTTGAAAACTTTGAGGATTTCCTTGCCACTCATCCCAAAGGCTTTGAGCAAGGTGGTAATCGGGAAGATCTTTGCCCCTTCACGTACAGCATCCCGGAGCAAGATTTGCACAAAGGCATCTTCAATCTTCTTTTTATTGGAGATGTAGATCAGCATTACCGAACCGTTGGCTGGTAATACTTCACATACATAACCTTCTTGTACCCGTTTACGCGAACTAGACAATTTTAACTGGGACTTAGTGAGCATGTAGATCCCCGGTGAACGGGTAATTTGGGCAATCACAAACTTCTCAATCCCGTTGACAATAAAGACCCCATCGCGGGTAATCAATGGTAAGTTAGTTAAGAAAACGCCATCGACACTACTGGTGGCACTGTTCTTCTTGGATTTGCGCGGCTTTTTAATGGTTCCGGTAGCGGAATCAATTAATTCTAAGTCAGCGTAAATACCAATTTCATAGGTCTTGGACTCAGAGCGTGATTGGGCTTCATTACGTTCAGGAGCGGTACGACGCAAACCCTTAAAGTTGATCGTGTAACGATCATTAGGGGACTTAATTGGAAAATAAGCAGCAATCAGGTTTTCCAGATCGTGATCTAGAAAAGTTTGATAGCTCCTAATCTGAATATCAGCCAGATTAGGTTGCACAAAATCAGTGGCAATTTTGCCATAGTAACGGCGGGTAGCAGTGGGTGAGTATTTTTTCTGAAAGAAACTAGGTTTTTGTGACATTCGTGTACAAAAAAGGGCTAAAAATAACAGTAAAAAGCCACTAACAGCGACAAAAGGGCCGCATGCTAATGGGCTTTGTAAAGTTTGCTACATTATATCAATTTTTGTACGTCAGTGATAAATGGCTGAAAATCAAAGCCATCATGATAGCCATTTACTAACACGGCTCCCTTAACGTTGAGACCACTAAAACTAAAGAATTTAGTGAGTAGTTCCAGCACTGTCTTACCACCACTCTTAGCTGCAGGAGTGACACAGCAAATAATTCCATCAAGCTCCTTTAAAAACTGGTTTTTACCGTATTGGGTATCGCGGGTCATCCAGTCGATTGTGTTTTTAGCAAAGGCAGGGACAAAACCGTTGTGTTCTGGAGTGACAAAAATTAGCGTTTTGTGATCGTGTAGTTTGCGCACTAAACTCTTAATTTTTTCGGGAAAATGATCCTTTTCCAAATCTTCACAATAAAAATCCACTTGGTAGTCAACTAGTTCAATTAATTCAGCATTGAGTTGTTGTTCCAAAGCTTTAGCAAACTTCCGGTTAATTGAATTTTTGGAGTTAGCTAACAATAAGATTAACGGTTTGGTACTCATTTTTTTATCTTTGATCCTATTTATATAAAAGAAAATTGTTAATTAAAGCGGAGAAAACTTAATTTAATTTTTAGTTTTCTTTTTTGCAACCAATTTCGCTTTAGCAACCCACTCCTCATTACTTTTCCTTAATTCTTGAGCTTTTCTTTCTTTGATCAACTTAATAAGTTCATTGATTTTTGCTTGTGCTTTTTCCTTATAAAACTCATCAGTAGCTCTTCTAACAGCTTGCAGTTATTCTCTTTTATCGTGATCAAAGGTGGTATATCATTCTGAGTCCAACTCTAATAAAGCGTCTTTTTTAGCAGAAGCTAGTCGTTCTTTAGCTTCTTTGGCATTTCAATGATCTTCTGATGGTAGTTTGGTGTCCATTGAAGATTTAAGGTACTTTTTTAATTCTTTGAGATGCTTCTTATCTTCGTTCTCTGATCATTTTTTAATGCTTTTTAAATGCTTAATTAAATCTTTAGACTGATCGTCTTCTTTAAAGCTTCCTCCCTTTCTTAGACTTTTGAATAAACCTTCTATTCTATCCAATACCTCTTCTTTATGATCATCCTCTAAGTTTTCCTCTAAATTTTTCTTAAATAAGAATTGAAAACAATAAACATCTTTTTTTATTAGTTTCACAACTACCAAAACTTTCACAGCTAAATTTATCAGTGTCTCACTTTTTTAATTCTTCTTTCGTTTTTAATTCAATTTCTTGAATTCTTTTTTTTGTTTTGCTTGTTCTTCTTTAGCTGCACACCCTCGGAAAAACTTATTAATCTCATCTCTCTTTGAGTTTTCCATTTTTTTCAATATAAAACAAAAGAGCACTCCACTAATGTGAAACGCTCAATTGTTAGAGGCAAATACTTATACCTCTAAATCTTTTGAACAGATTTCTATTAATCTATATGCTTGAGAAAGATATGGATAGGGTCTCAAGAAAAGAACATCTGAATAATAAAGCTTTGAAGTTTTAAACTTCTTAAACCCTATATTCATTTTTTTCAGAAACTCTTTCCAAATTCTTTTCTTAACCACTTTATTTTTAGTGCTTCAGATATCACAAATAAAGCTAATACGCTTTCAAAGTTAATGCTTTTAGTTTACTTTTCAATAAAGAAACAAGGCTCGTATTGCTTGATGTCACCTATTAGAGTATTAGTGCTTACGGTGGTTAAACCAACGTTTTGATCTTTATTGGCCTCTAATAAACAGATGCAATTATTGTCATAGTTGTAACCCTTCATTACTTCAACAATAAAATAGGCATCTTGTTTAATCACAATTGCATTAACTTGTAAAGTGCTAGCTTCTTGGGCAAACACTTGCAAGTTTTCAAAATACAGCAATAATTGTCCCGCAAAGTTAATAAAGGCTTGCTTTCAAATGGTATTGTTATCTATTTGTAAGTGGTGCTTAATACTCTCCAAGTTTTTGAGTAAATACTTGTGTGGTACGTTGAAGTGCACATCAATGTCTAAGTTATTTTCAGTATTAAAGCGTTCTTTGAAACTGGTAAAGCTCAGGTCAAAACTGTTACTGTTAGCCATAGAAGTGCCAAAAGCAAACTGTGAATTTAACTCGACAAAGAAAAGGTTATTCTTTGTTTTTTGCTTGCGCTTTTGGTGGTTCCAAGTATTAAAAAACTTCTTTTCAAAGTTTTTGCGGCAATAACGTTTGTACTGATAACCGTGCTTTTTAATGATTTTAACGATTTCATAAGAAAGCTTTGTTCGCTTATTTTCAAACGGAGAATTAACTAGTAAAACCACTAATTTCAGCCCGGAAAACTGTTTAACTGCTTCTAACAGCTTTTTAATTTCTGTTTCTGTTTGTTTGGTTTTTTCGACCAACATAAACACTGTTTCCGCTTCTTTATGTAACCTAATCGCTAAACCAAAACCACTTAATAAGTTATTGTCAAAACTTAAAAAGCGAAAGTGATCAAAGAAAACGTGTTGGTAAACTGTTTGACTCGCTGATGGTACTAATAAGTATTTAATCTGATTCGACTTAACTAGGTGATTGACAAAATCTAAGTATTCTAAAGCGGAAAGTAATCCCACTTTATTGGTGTTTTGAAAGCAATCGTAAGCTAAAAGTCTTTGCTCATTCACCAAGGTTTGCTCGTTAATGGTTGTTTGTGCCATACCTAGTAAATTTTACTTAGTTTATTATGCTTCAAAATATCGTGCATTGCTTTACAGAAACTTTCATGCTTGCTGTTAAACATTTGGTGTTGACTGTGTAAGATAACCCCTGATTGCAAGTTACCTTTCTTAATCAAAAAGCGGTTAAAGTAGTTTAAAGTAGCTTTTGTAGGCATAAAATCATCATGTTGAGCCAACAGTAAAAAGGCTGGTATGGTAATAGTTTTATAAGCACTGTTTAAGCGCGACAATTCCTTAAAGATCTGAAAGGTGAGTTTTTTGCACATAGCAGTATGCTTGTCTAACTCCATTTGTCACTGTCCATCGTTTTGTAAATCAGGAAACTTTGGTTGAAACATCCGCTTAAAGTAAGACAGCTTTTCGTCCTTCTTTTTAAAAGCCTTTTTAATCTTGTAGTAATTCAACAAGTTGGAATAAGACAGTGGCGCTAAGAGCACTAGTTTTTCCACTCGTTGTGGTTTTAAAAACTGCAATGCATAACTAGCCACTGCCCCACCCATGCTGTGTCCTATTAAAACAATGTTTTCTAAATCTTGCTTTTCAATTCAAGCTACCAATAAATGGGCATAGTGAATAGGTGACAATTCCTTGTCAGTAAAACCATGGTGGTTATGACCCGGTCACAAGGGTGCGTAATAGTCATAATCCACCAGCGTTTCAGAAAAGCTCTTAAAATAAGAATGGTGCACTGAAAAACCGTGCGCAAACACAATAGTTTGTTTAGCGGGTTTTTTAGCTGGTTGAAAAAATACTTCTAGTTGGCTAATATCAACAGTATTAATATTGACTTTGTCTCTCACAATTACCTTTTAAGAAAAGTAAAACACCTTGCACACAATTGGTGTCCTTTTTTAGGGTAAATTTGGGTTTGGAAGTTCCAACATCGTTCACACATGACTTGATCGGTGTGTTCAACCTTAATTTCGTTGGGGTCATTCGTTATATTTACCTGGTTTACGTTTAGCCAAACTGCTAAATGTTTTACAAGACGTTGATCAATTGCACTAGTAGAATCCACACCCAATGTCAATACAACCTGGTTGTTTTTAGTAATCACACCATTTTGACGTGCTTGTTCAATGGCTGCATAAGCCCGGTCTTTAATGGCGCTAAAGCTTTCGTGTAAGCGATCTAACCCTTTAACATTAGCAACTTTAAACATGGCTGGTCCTGCAAACAGGTTTACACTAACCGGCTTTTTAGGCAAAAGATAATTCTGTCAAGCATCTTCGGCAGTATGGGGAATAAAGATATTTAAGAAACCAATTAACTGGGTAAAGATATAGTTCAATACTGCCTGCTTAGCCAACCGATTAGGATTAGTTTTAGCATCACAATAAAGTGTGTCCTTAATAATTTCAAAATACCAACCCGATAACCACAACACAAACTTATTAATGGCCTTTAAGCAATTTAAAAAGTTGTATTGCTTTAACCACTGGTGCAATTCTTGTACCAATGCATTAGTTTTATGGATTACGATTTTGTCCTCTAGAGCAAAACGATAATCCTTCATCTCACCAAAGTTAAAGTGGTTGAGGTTACCCAATATAAAGCGCAATAAGCTGTTGCGAATGCGGCGGTATTGCTCGACAATTTGCTTGATAATGTTGTCACCTATGCGGTTGTCCACTTGTCAATCAACATTAGTTACCCATAAACGCAGAATGTCTGCGCCATACTGATCACACAACTTTAAAGGGTCGACCACATTACCGAGTGACTTGGACATCTTATTACCATGTTCATCCAAGGTAAAACCATGGGAAATCAAACTCTTGAAGGGTAATTGGTCAGTTTGGATAATACCGCAACTGGCGGATGAGTTAAACCAACCGCGGTATTGGTCACTACCCTCCAAATAAAGATCAGCGGGAAAGTTCAGTTTATTACTAATTAAAACGTTGTAAGTTGAACCAGAGTCAAACCATACTTCCAAGGTATCAGTTTCCTTTTTATATTCAACTCCTGGCTGTATTTTCTTTGGGTTTAAAAAAAAATTAATATCCTTTTCAAACCAACTGTCAATCCCGTACTTTTTGAGCTGTTGAATGGTGTATTTAATGGTAGTCACATCCAGTAAGGGTTGGTTATCTGCATAAATTAAGGGAATCGGCAAGCCTCATACTCGTTGTCTGGAAATACACCATTCAGCACGTTGCAACAGCATTTCTTGTAGGCGCTCTTTATTCTTGTTATTAACAAACTTAACCCTTTTAATTTGACGCTTTAGCTTACTTTGAATTGATTTAGTTTTAATGAAGAGCTGTTTGGAGGCACGGTAAATCACCGGAGTCTTACTGCGTCAATCGTGGGGTTCGCGGTGCAAAAAGCTTTCACTAAAGACAAAGTTATGGTGTTGCTTTAAGCGTTCAATAATCACATCATTAGCCTTTAGATAAAACAAACCCGTTAAAACTGGATCGTTCAACGTATCATTAAAAACTCCTTTTTCATCAAGTGAAACTAGCGCTTCATTTAACTTGTTTTGCTTGCACAAATAAAAGTCATCCAAACCATAAGCTGGTGAAGTATGCACGAGTCCTGTACCCTCATTACATAAAACGTGATTACCCAACAACACCGGGTTCACTTTGTCATAAAGACAGTGTTTATAAGTAGTGTTTTGTAATTGGCTACCACTAATGGTTTGTACTTGTATAGCATCAGTTCAACCAAACTTAGTCTTAAGCGATTCAAATAAACTGGCCAACACCACGTACTGCTCGTTATTATAAGTAAACAGCAGGTATTCAAACTGGGGGTGAACGGCAATTGCTTGGTTAGTAGGCAGCGTTCAAGGAGTAGTGGTTCACACTAAGAGTTTAGTACCTGAATTTAATACTGCACTCTGCACTACTGTAAAAGTAAGGTAAAGTCCAATTGTTTTTACTTCTTTGTATTCAATTTCGGCCTCAGCCAGAGAAGTGCGTGATACAGGCGATCAGTAAGTTGGTTTTAACGCTTGGAAGATTAAATCCTTTTTCACTGCTTGTAAAAACAGGTCTAGCTCATTTTGTTGAAAGCTTTCATCAATTGTTTTGTAATACTTACTAAAGTCATTCAACAGACCTAAGCGTTGAAACTGGGCTTTTTGAATGGCAATTTGTGACAGGGCAAATTGCTTGCATAAATCGCGCTTTTCACTCAATGATAAGCTCGCATAGTGTTGCGGATCTTTTTTGCTCACCGCATGCTCTATGGGCAAACCATGGCAATCCCAACCAGGAATAAACACTGTACCAAAGCCTTGTAAGTTTCAACTACGGAGCACAAAGTCCTTCAAAATTTTGTTTAAAGCATGGCCAACATGGATATTGCCATTAGCATAAGGCGGACCGTCGTGTAGGATTTTTTGTGGTTTATCTTTGTTTTGGCGGTGCAGTTTTTGGTAAATCCGTTTACTTTGTCAAAAAGCTTGAAACTGCTGTTCCTTAGTGGTTAATTTGCCCTGCATTTCAAATGCAGTTTGGGGCATTAACAGTGTCTTTTTTAAGTTCATTAAGCATAGTAATTTTATTAATATTTAACTAACTTCTATCTGATGTATAAATCAGCGATTAATATCGTCTTATTTTGCCCAGAAATTCCTAATAACACTGGGAACATTGTTCGGAGCTGTACCGCTTTTAAAGCTAACTTGCATCTGATTAAGCCCTATGGCTTTTTTCTCAATGATAAGCGGATGGTGCGTGCTGGGTTAAATTGTTGGGATAAGGTGCAAATGTTTGAACACAAATCCTGGGAACACTTCATTGAAACAACACCACCAAATAAAGCAATTTGGTTGTTGACAAAGAGTGGTACTACTACCCCAGACCAAATTAACATGCAAACAGATAACAGGGAACAACTGTACTTTGTTTTTGGTCAAGAAACCAAGGGATTACCGCAAACACTCATGGAACAATACTCGCAGAACCAGGTACGGATTCCAATTTGAAACCCTGTAAGAAGCATTAACTTGTCCAATACAGTAGCTTGTGTACTGTATGAGTATGCTAAACAAAATCATTACTTTAATTTAGATAAACAGTGCGCTTAAGAAAAGTTAAAGATGCTCTTGTAAGGGTACAAGACAGCCCTTACTTTAGTAACCCAGAACAACTGTCAACTATTGAAAGTGAATCGCTCTTCGTGGAAATAGGTTGTGGTAAAAGTTGGTTTTTAAGCCAGCAAGCACAGCAAAACCCAAAGTGTTTTTTTGTGGGAATCGAGCGTGAACCCACGATTGTTTTAAAGGCAATTAATAAGGTAAACCGCTTAGAGGTTAAACCACGTAATTTATTAATAACGTGTTTGGATGCCAACCAGCTAACAGAGTACTTAAAACCACAATCGATTAGCAAAATCTTCATTAACTTCCCCGATCCTTGACCAAAAAAGCGTCACACGTTGAGGCGACTAACTGCTCCACATTTCCTCAAGCAGTTTCACCAACTTTTAAAAAAGCAAGGTTTAATAGAATTCAAAACGGACAACGATCAGTTGTTTGAATTTACCTTAGAAGTCTTGCAGAAAACAGTAGCTCACTTTAAGATAATTGAGCAAACGACAGATCTGCACAATTCACCTTTGGCTAGTACCAATATTATGACCGAATACGAACAGCGCTTTGTTAGTTTAGGGGTTAAGATTAAAAAGCTAACGCTTGAAAAACTAAATTAGTAAAAATTAGAATAATTACTGCCTTAATGGCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCATTAAACATACAAGCAGCAGATTAAACTGCTGCTTTTTTTATTTAGATAACTAGTTTAAGGGTGTGTAAACACTATCCAAGAAATTGAGAAAGTTAATGCTTACCTGCACGGCATGGCGTTTAACGCCAAATGATCCAGCTGAATAGGATTTTTTAGCGGCGGCTGCAGCAGAAGCTGCTGGAGTTGTTGAACCGTTATTTCCACTGTCCTGATTTGGTTGGTTTGAACCTGGTGCATTACTGCCGTTACTGTTATCAGGATTATAGAAATGCGCACTAATTTGATCTAAATTGTTTTTGCGAAAAGCAATCACAATTGCTTTAGTTACATCAGTGCCAACCGTTTGGGCATGAACATTGGACTGGTTAATAAGATTATTGACACTACCGCTGTAAACTGTTTGAATAAAAGTCACAATTTCGCGGTATTGAATGGCAGATTCGTCGTTTCTTCTTAATTTGTCGGAATTGTCCTTATCAGTTTGCTTAGCTAATTCGTTATCACTAGCGATAACTGGATAACGTGCAAACGGATTCCACTTAACAGGGTTAGGATCAATGTCATTCCAAATTAAAAACTTAACTCCTTTAGGGTAAGTTGAATAACGGTTTTGAACCTGCTTAACGGTAGTACCTAGATCACCAGCTCAATTTTCGGTACTAACAGCTTGATTGTTTGAACCATTTAGAAAGTTAATTTGAGAACTGTCAGTTTCAGTGGTTACTACAAGAACGTAGTTACCATCATTGTATTCCGCTGTTCCAAAAACTAAGTTTCTAACCGTTTTATTTTCTTTAGGTAGCTTACTGTAATCAACTTTGTTTTCTACGCTTTGCTGTGAAAGAGTGCGAAAAACATTTGGTTTATCAATATTGCTACAGGAACTCAAAACAATTCCTGAACCTAACAAGAGTAAGGTTGAAAAAGATACTTTCTTTTTAGAAATTGAACTAAACCACTTCTTCATTCAAATACTAGTATTGGTGTCGGAGACCAGACTTGAACTGGCACAGTCTTGCGACCACAAGCCCCTCAAGCTTGCGTGTCTACCATTCCACCACTCCGACGCTGGCGTCTTATAAATTATTAATAGATTTTAAAAATCCATAAACTCTTGATAAGGAAATTTTGCATTTTCTCAACTTTGTGCTTTGTAAATTAATCTATAAAACACAATCTTTTCATCACTTTGACGCTTTTTAAAACCCAACTCAATTTATTTAGTGAGGGCAGGAAAACGAAAATTTTGTGTTGAAGGCCTGATTTTCTGTTCCCAAAAAATGCGTATTTTATTTTTTCATTGTTTTCTAAATTACTTATCGTAAAGGCTTTCAGCACAATTGATGTATCTCTTTTTTTAAAAGAATAAAGGTCATATATTCCTTTCATTCAAATCACTAAAAGTTACAACTTGCTGTTAATTTCAAAATTGTCAAAAAGCCGTCTGTTTTTTTCACCTTGGGCAGATGGTTTTTTTATTTGTGTGAATTAAATAAAAATACCAACAGCACCACATCTTTAGGTTTCACTGTTGGTTTTTTATTTCTGAGCCTTGATTATTTGGACTCAAGTTTGTCTATTCTTTTTTCCATTTTGTCCATTTTTACTTCCATCTTGTCCATTCTATTTTCTATTTTGTCTATTTTTCCTTCCATCTTGTCCATTTTTACTTCCATATTGTCCAAGCGATCGCTCATCTTTTGCAGCGTTTGCAAGATTAGCTTTAAGGTTTCGCCCTGAGCTTTTTGCTCGATTTGAAGTTCTTTAATTTGCTCACCCTGGATTCTTATTTGCTCACCTTGGGCCGCCAAAGTGGTTTCAATCTTTATGAGTCGCTGATTGTTAGAATCTTTATATTCCTGAAATTGTTCTTGGGTAACGTATTTAACTTTCACAGGTGGTTTAAGTTTATATTTTCGCAATTCATAAGTTCAAACTCCAACTGTGCCATCACCGTACTCTTCTTTTTCACCATCAAAGTGTTCTTTTCCAATTTTACGGATTAAGGCTTTGTATTCCTTTTCGGAAATTTTTTCTTTCATTAAGTAATACCTCCTTTCTTTTTACAGAGGTTGAGAAAACTACTACTTGCAGCAGTGCTGCCAACTAAGTTCAGCTGTTTTGCTGATTTAATCAATTTGTTTATTAAAGGCATTTTTTGTCCTCCACTAAATAAATTGAGTAGGTTATTTAGAAGTGTCAAGAAAACAACAAATTTTGAAATAGACTAAATACCTTTGCTAATTCTTAACAGTAAGAGAAAAGAAGCCTTGTTTAGTTACTAGCTATCTATTGCCATTAGGACAACAGAAACAGTTTGCTTGCATTTAAAGGATTTAAATCTCTAATAAATTACTTATCAATGACACTGAAAAACATAGCTTTTTTGTTTTTATTACCCTTACTTTTTCCAAAAATTCCACCTTTTTTCTACCTCAATTTTCAAACCATCTGAAAAATAAAAAGTTATTAACAAAATGTTGATAAAGACCTAATTTAGTGCATTTTTGTGCCTTTTTAGTGTTAATAACTTGTTTAAAACTGCTTCTAAGTTCTTTTTGGTAACTATCAGACTAAGCCAGATTAGCAAAAAATGTTTACGAGCCTTATACTGATTAGCGTCTTTGCAGTTTTATGCAGCCGAACTATTACCGTGTCATCAAGAAAGCAACCTCATTTTCAGGGTTAGATATGATCTCTCATGCCTATGGTCAGATTGCTGGCGTTGAAGTTGTGGGTTTTTATTTATGACTAATTACTGAAGCCAATGCTCAAGCCTTTAACAGTGAAATTAGAACGCCTATTTCACGTCTACAAAACGCTTTCAATACTACTGGTACCAAAAACGTTAATGTACCTGATTGAATCTATAAAACCATTGGTAAATTAGAAAGCTTAGGTTTGGTGCGTACCTTCTTTTCACAGGAAAAATCAGAAATGACTTTTTGAATAGTCGAACCATTAGGTTGAAAAGAGTTTAACCAAAAGAAGCACTTTAAAGAAAAGCTAATTGAATCAATGGGAAAACTGGAATATGACCGCAGCTGCCTTTCCTTTGAACAAATCGATAACATTCAGTTTGACAACGCACTGGAAATTACCGCTAACTTTGAAGCTAACTTTACCAGTAAGCAAGATTGTCTTAGCTTTAGTTTTGACTTTGAAGCTTTTCATAAACAATTAGTTAAACAAAATCTCTTTGTTAGTTTTAACCAGAAAACTAAGGCTGTTATTAATGGTTACTTTGAAAAATACCAAATAACACTAGAAGGCATATTGGAATGTGTTATCCCTAGTGTAGTTAATGATGAAGTTGACTTGGAACTGCTACAAAAATTACTAGAGCAGCTAGTTAAAAACAACACAGCTCCAATTGTTGATACTGTAATAAATGACCGCAACTTCTTTTATGATAATCAGAATTTAGCCACAGAAACAAAAGATGCAATTTCCCGTTGTCATTTGGAATACAACGCGGAAAAATACCTATTTCTCTTGTACGGTAAAGTAGAAAACGAGCAGTTAGATCTAATTCGCCGCTTAAGACAAGAGTTTGGAATAGCTGATAAGGTAATTAATCTAATAGTAGACTTCTCCTTTTGGAAAAACAACAGCATGTGACGCGAACAATACATCTTAAAGATTGCTGAATCAGTAGAGCGTTACCGTAGTCATAATAATTACCAAGCCACCCTTGATAACTTTATCCGTGCTTCTAGTTTAGCCAAAAAGCAACGTACCAAAACTAAGATAGAAAAATCCGAGCCTGAAACTAGTGCTGCACCAGTAAATGAAACTGATGACGTCAATTACTTTTTAAACCGTATTAAAGCAATCAACAAGAAGAACAGGGAAAATGGAAAACACAAACGCTAAAACTCAACCAGCAATAAATGAGTTAGATAAGACTACAGTTCAAGACACAATTAAGAATTACGAACAACAACTTCAAAAAGAGCTTAATGAAGAACAACTAAAAGCTTTTAAAAAGCGCTTTAAGTATTTGAAGCTAACTAAAAGAGAAGTGATTGAAAAGGAAAGCAGGGAACTTTTATTGCAAGCATTTAGAGACTTTGAAAATTGTGAAAAGATAAAAGGCAATCATTGTCCTAATAAAGGCAATGCTCACCTCAACATCTTTCGCGAGCCACCTTTTTGAACTAAAAGTAATAAAAAAGTACTGGGTAAGTTAATTGGTATAAAAATGTCCTGCGCTAAATCTATTGATAAGGAGGATATCTTTAAATTGCGCTATAGTAATTTCATCATCTATAAGCCTAACTGATTTAAATACGCTGACGAATTTCCACAAATTAACCAATTACCCATTAACAAATTTCAAGAAGAATCTTTCGAAGAAATAAGAAATGATCTCAGTGAGCTTTATGAAAATGAAAAAAATCACAAGGAAAACATAAGTTTCTTTAACTTACAAAAAGTTAATTTCAATCACGAGGGCGCTAAATTACTACTTTGTGATTTTGTCGAAAGAGGAGTTAAAACTGCGTTGCTTTATTGTGAAGAATTTGTCAGTCGTTTTGACCGTGCATACTGAAAAGTAGATGATTATTTAGATTTAATCAATGAAGCTAATGTCATCATATTTGTCAGTCTTGGTGAAGAGAGTTTTGTTTCCAAAAACTACATTCTCTTCTTAACCAGATTATTCGACATCATTAATTCAAAACGCAAAGATGTTTATTTCTTTAGCACCGAATACAATGAGAAAAATGGTCTCATTCAAGCATTTCAAAAAAGCATTCACTTTAAAGCTAAATGAGTAAATTCCTTTTTTGAACAACTCAATTATTTTTTTGAAATGAAAAGAGAAGAAATTAAATAAAAAGCGTACTAATTTATTTAGTAACGCAAAATTATTTAAAATTAATCGTAATTATTTCCCCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCATAATTAAATACTTAAATTTACACTACGAAATGACAACCTCAACGACCGTTGGGGTTGTTTTTTTATTTATTAAAGGTAGTTTAAGTAACGAACAAGATTCCTGCTACTAATAATTAATAGACAATCTGCGCTTTCTTTCAGTAAAACTTTTGGAAAGCACGCATCACTGGAACACCAATTAAACTCACTAGTGTGAATTTAATCACATTAAAGAGTGAGTATAAAGCAAAGATGCCAACCCAATAATTAGGTACGCCAAATAAAAAGAAGTGAAAATATGGTGTTTTGTTATATATCTTGGCCACTTCGACAAAGTTAGCAGTCGGCACATAGCCAAACCAATACCAGTATAAAGGGGTAATTAACAAACCGTTGAGCGTTGTTAACAGCAAAGTGTTAGATAGAGTAGCCAAACTAAATACCCATAACCAACGAAGTTTAATTTGAGCAAAAATGTGAAAGTACAACCGTGTAAAACTCACGATAGCAAAGTTAGCAATGGTTAGGGTTAAAATACCAATCCAGTTATTACCATCCCAAATAAAGCTACAAAGTGACAGCGCAACGGTAATAACTAAGGCTCAAGAATAAGAAACAAAAAAAGCACAAGCAATTAAAAAGACTGATGAAATGTCAATCGTTAATTTAAGTGCTGACAAAAACGGAATACTGATAAAGTGACTAACAAACGAAGCAATTAGTGCAAGCGCTAGCATCATGGCACTAAAACTAAGGTTAAACAGTTGGTGTTGTTCTTTTTTTGGCCAAAAAGCTATTCTTGCACCATTCATTATTTAGTAAAGCCTACTAGCTAGAAAAGTAGTTAAATAATAATATTTATTAATATGGACAAATTTTTAATCGATGTTACTGTAGAAATTCCTAAGAGCAGTAAGATCAAGTATGAATACGACCGCAAGACTAGTCAAATCCGTGTCGACAGGATTCTCTTTGGTAGTGAATCTTATCCACAAAACTATGGTTTTATCGCTAACACCTTAGACTGGGATGGTGATGAATTGGACTGTTTTATCTTCGCTGACCAAGCCTTTCTACCAGGTGTAGTAGTACCTACTAGAATTGTAGGAGCTTTAGAAATGGTAGATGACGGTGAATTGGATACCAAACTTTTAGGGGTCATTGATTGTGATCCGCGTTACAAAGAGATTAACTCCGTCAATGATTTACCAAAACACCGCGTTGATGAAATTATTGGTTTTTTCAAAACTTACAAATTACTACAAAAGAAAGAAGTAATTATTAAGGGAGTGCAAAGTTTGGAGTGGGCTAAGAAAGAATACCAAGTGTGTGTTGATTTAATGAAGCAATATGGTAAGTTGCCTAAGGATGAGTTTATCGCTAAAATGCAAAAACTCCATCCTGAACACTACCAAAAATAGTTCAAGTTATGATTAGCGCTAACCGTGGCATGTTTTTGGAAACAATTGTTAACCAAACAATTGTACGACTAGGAGATCATCCAGATATTTGACTCGAAAAGCGTTATCTACCGATTCAACCAACTGCTTTTAAAGAAACCCGTGTGCAAGGAGCATTAACAGCGCAAAAGAAATCGAAAACTGACTATTACGGTGTTTATAAAGGCGAAATTTACTTTGACTTTGAAACTAAACAAACTAATAAAAGTAATTTTGCTCTAGCGCAGTTAGCAGAACACCAACTCAAACACCTTAAAAGAATTAATGAGATTGGTGGTATTAGCTTTTTGTTAATCTATTTTCAGGTACAAGACCTCTTATATGCGTTGCATACCAAGGATCTCTTGCAGATGATCGAAGCGCAAAAGGGTAAAAGTAAAACAATTAAGCGTGACCTTATTGATCAAAAAGCACAAAAGATAAATCTAATCTACCCAGGGGTTATAGATCTAATTGATGTAATAGAAAACTTTATAAAAGACTCTTAGTCTGCGTATTTTCAGCGTACCTTAGCAGCCGTTTCCTTAATGGTTTTGCTTGGACTGTAACGCACTAATGGTTTTGGTGGAATGTGTTTCATTTCACCAGTTTTCATGTCTTTTTGAATTCTAGCGTTACGAATCGTAATGCGTAATTTACCCAAATTTTCGGGCAAAATGCAAACGCTACGACTTACTAATTCATTAAGTAAGAGGGTGTTGAGGTATTTAAAGATCTCTTTTATCTTAGCCTCTTTTACTCCAGTAGCTACTGCAATAATCTTGTTAATTTCGCTACGAGACAATGGCTTACTCGATGTTGTTGTTTTTTCCATTAAGTTTTAAGTTCAAAGTTTGAAAATCCAATTTGATTATAAAAACGGAGGAGAATCAAATTAAACGCGTTTTATTTTATTATTGTTCCAATCTTTAAAAGGTTGCACTTTTAAAGGTTAAATTTGACAAAAAGTTTTCCTTATAAGTATGGAACCAAATAATTTAAAAGAAGAGCTAGTTTCAGTCTTTGAAAAAGCTTGTTCTTCACATAAAGAAAGGCTTGATTTTATCTGTTCTGTAAGAGAAAGCGATACCTTTTCCAATGTGGATGTACCGCTAGCTCCTATTAAAACTATTATCGAAATTGCTAAAAACGAGGAAAATCAAACCGAAATTCTCAAATTAGCGATCGAAAACATTAAGACTCTATCAACTGTTGGGTCTGGTCAATACATTGCATCGCACTTCTCAACCCATAACGAAGTTGCTATTATTTTCTGTATCTCTTACTTTTTGTATCACTTCAACTTCTTGCACGATGAAAACAAAAAGCAGTTGCTAAAACGTGCCTTTGAAGCAGTTGCTGAAAAAATAGCTGACTACTTAAACGAAAACTAAGATTTAACCTTATTGGCTACTATTGCCACTTTATCTTTCTCAACTATTACGTTGTAACTAACATCTTTAGTAATAATACCTTCCAGAATTTGCTTGGCAATTAGAGTAGCTATTTGCCGATCGATAAAACGTTTAATTGGTCGAGCACCAAACTGTTCGTCGAAACTAGATTTGTAAATAAACTCATTTAACTTAGGGTCAAACGTGAACTTTAAGTTTTGTTTAGCTAATCTTTGATTTAATTGATCCAATAAATTTGTAATAATTTCTAAAACGGTTGTTTTTTGCAATACGCTAAAGAAAACGATTTCATCAATCCGGTTAATAAATTCAGCACGGAAGTGTTTTTTTAGTGCTTCCATCGCTAAATCGCGTTTGTTTTCCATAATGTAATTGGAACCCAAGTTGGAAGTCATAATTATCATCGTATTCTTAAAGTTAACTAAACGACCTTGAGAGTCCTTTAAAGTACCATCATCTAATACTTGTAAAAGAATATTAGTTACATCAGGGTGTGCTTTTTCAATTTCATCGAACAGAAGCACACAGTAAGGTTTGCGTCGGACTGCTTCAGTTAGTAAACCAGATTGTTCATAACCTACATATCCAGGAGGCGCTCCGATTAACTTAGCGACCGAATGCTTTTCCATGTATTCACTCATGTCAAAGCGAATCATCGCTTTTTCATTATCAAACAAGACCTCTGCTAGTGATTTAGCCAATTCCGTTTTACCAACACCAGTAGAGCCTAAAAAGATAAAGGAACCAATTGGCCGGTTAGGGTCATTGAGGTTTACCCTTCCACGCATAACGGTATTGACCACTGTCTCCACGGCCGCATCTTGACCCTTAACCCGTTTTTTTATTTCATCACCTAAATGCAAAAGCTTTTCCTTTTCGGTTTCTAACAGCTTCTTTAAGGGAATACCGGTAGCTTGTGATACTACTTCGGCCACTTCGTTTTCTGACACTTCTGTCTTAAACAAGTCATGTTTGGAATCAACATACTTTTTCTGTGCTTGTTCTAATTGGTTTTTAAGTTTGGGGATGTCATAGTAAAGAATCTTAGAAGCAGCTTCATAGTTACCTTCCGTTTGGTAGAGCTCTAAGCGTGTTTGCAAATCCTCAATTTCCTTTTTGAGCTTGTTAATGCTTTCAAAGTTAGTTTTCTCCTTTTTTCATTCGGCAGTTAGAGAATCACGCTTTTTCTTTAACTCATCTAACTGTTGTTTTAGATTGTCTAAGTGTTCTTGTTTTTTCTTGTTATCAGCATTTTCCTTATCTTGTTTTAAAGCGGCATATTCAGTTTCTAAGTTAATAATTTCCCGCTTAAGGGTGTCAATTACCACTGGCTCTGAAGCCATTTCCGTTTTAATCTTGGCACTAGCTTCATCAATTAAATCAATCGCTTTATCGGGTAAATTACGATCAGGAATATAACGTGCTGACATTTCTACAGCAGCTACCAAGGCACTGTCAAAGATGGTGAGGTTATGAAAAAGTTCCCAACGGGTCTTCAAACCCCGCATAATGGTAAGTGCTTCTTGTTGACTCGGTTCACTGACAAGGATCTTCTGGAAGCGCCGTTCTAAAGCGCCGTCCTTCTCCACATATTCACGGTACTCTTTCAGTGTCGTGGCACCAATAACCTTAATTTCACCGCGTGCTAACATTGGTTTTAAGATGTTGGCAATGTCCATGGCACCACTACTGGAGTTACGACCTAGCCCCACTATTTGGTGGATTTCATCAATGAATAAAATAATCTTGCCATTTGATTCTTTGACTTGCTTTAAGATACCGTTAACCCGTTTTTCAAACTCACCTTGGTATTGGGTGCCAGCAATTAAACCAGACAACGAAAGTTCATAAATTTCAACATCACGCAAGTTTAAAGGCACGTCATTATTAACTACCCTTCTTACAAATCCTTCAACAATGGCGGTTTTCCCGACGCCTGGTTCACCAATTAAAACGGGATTGTTCTTGTTTTTCCGGCTTAAAATTTCAATTAAGCGTCGAATTTCGTTGTCGCGACCAATAATAGGATCAACCTTATTTTTGAGCACCTCATCGTTAATGCTACGCCCCATTTCCTTTAAGAAATCACGCTTATCTGGTGTTGGTGTAAAACTAAAATCCATCTATTTTATCCCCTTTCTTTGTTAATAGGCCAATTATATAAAATTAGCACTCGAATGGCTTGAGTGCTAACGAGTGGGCTAAAGATTTACCTTTTTTGGTGGTTATAAAGTAAAAGAGAGCCAACCCCCAAGTGCTGCTCTCTTTCATTTATAAAGACGGTTTTGAGTTACTTAACTAGTACAACGGAATTGTTTTGGCAATCTTCCAAGTGTTAGGGAACAGTGCCTTTAAAAAGTAGTTCAAGCGCTTTTGCACCCACTTTTTGTAATCAATAATTTGCTGTGTTTGAGGCATAATTTCAGTTCACTGAAAGGCAAACAGCAACACGTAAATTACCGTTTCAAAAACTAGTTTTTTGTCTAAGCTACCGTACAAGTCAATAATTTGGTTGGCTGTTTCAATTGTCAACTCTTCGCGAATAATGTTGGCAATCTCATAGTAAGGGTTGTCCACACAAGACCACTCAAAGTCAATTAAAACGACCTGCTTTTTTGGGGTATAAACCAAATTATCAAAGGTGGAATCGTGGTGACACAAGGTTTTGGGAATCGCTTTGTGTTTATCTGTTAATTCCACATACAGTTTTTTGTAAAAGTCAGGTAGCTTGGTTTGGTTAAAGTAAATTAACGGATCGAAGATTCAAATTTCATTAGCAAACTGTTTTCACTCGACTGCATGTACTTTTTTTAAAGAAGCCACTAGTTGTTCTAAAAACAGCCTTGTGGGACGTTTTGGTTGTTTTCCTTCAATCCACTTCTTAATGGCATTACCGGTAGTTACATCAAAGTAAACAAAGGGGTTACCAAAGATGGGGGTCACTAAGTCCAGGATAATTTGCTCGTTGCGTCGGTTCAACGGTACATCTTTACGCGCTAACCTGACTTGGTACTTACTCCCATCTTTCAGTTCTGTAAAAAAGGAAAAATTAGTAAAACCCGAGTGGATTTGTTCCACATGAGCTACAGCAGTTGGTTGGAGGTTTAACTTTTCACAAAGAAAAGCAATTGCATCGCCCTTATTCATCTATTAACTATTTTTGAGTTAGTCGAATGGAGTCTTCCACAATCATTAACTCTTCATTAGTACGTACCCGGTAAATCTTGTACTTACTTTGGGGCGTGGATACCACACAGCTATCACCGTACTTGACAAACAGTTTTAGATCAGTCGCAAAGCCTAATGATTTAACGGCGTTAATAAACAACTCTACACAATCACTAGCATTCTCGCCAATGCCACCAGTAAACACTAAACCATCAATGTTGCCTTCCAATTGGTTCAAGTATTTAGCAATGTAATCCGCTACCCGGTTAACATACATTTTGATAGCCACAGCGTTTTCTTGGGGTTTAGCGAAAACATCACGCATATCCGATGCACCAGTTAACGCCAACATCCCTGATTTTTTATTAAGCGCATTGACCACATCACTAGCACTCATGTTTTCCTGTTCTGCTACATAACCAATAATGGCAGGGTCAAGGTCACCACTACGGGTTCCCATAATAAGACCTTCTAGCGGGGTGAATCCCATTGAAGTATTAAATGACTTACCATTCTTAATGGCACAAACACTCGCACCGTTACCCAAGTGACACACAATCAGGTTCAGGTTTTGCTTGTTCAAGTGCTTTTCTAAATAGTTATTAATGAACTTGTAAGAAGTACCATGGAAGCCATAACGACGTACTAAGTGTTTTTGCTCCCATGAACGTGGTACCGCATACAAGTAGTTTTCTTGGGGAATGGTAGTGTGAAAGGTTGTGTCAAAGACTGCCACATTCTTAGCACTAGGAACTTCTTTGAAAAAGATTTCAATCACATCAGCCTCTGGCTTGTTGTGCAGTGGCGCTAACTTAATAAATTCCTTAATTTTAGCGAGTGCTGCTGCATCTACGATCACCGAATCCTTAAAGTAGTTAGCGCCCTGCACCACGCGGTGACCAACTAGAGTAATGTCACTAAGTTCCTGGATAATTTTGTGCTTTTTAAGTGTGTTTAAAAAGTGGGTTACAGCGGCGTGGTGGTCGGGGAAAGCTACTTTTTCTTCAACCTTCTGTTCGTTAAATTCAAGTTTGAAAAAACCATCGACAAAAATTCGTTCACAAAGGGCTTTAGCTAGGACTTTTTTGTGATAATCAAACAATTGGAATTTGATCGAGCTACTGCCAGCATTGACTACTAAAATTTTGTTGTCGTTCATTTTTATCTAATAGGTAACAATAATAAAATATTAGTAATAAGTCCTCATTTTTTCTCTAATAACTGTCAATGAGTGCTAAAACTTCATATATTGTGGCTGCTGTTTCGACAGCTGTAGGTATTATTCTCTACTTTATCTCTTCCCTCATGGGGGTAATTGGTGTAAGTAATGATAAGACCTTAATCATTGTCTTTTTTGTATTAGCTTCAATCATCATTATTAACGCCTGTGTTTTTTGAATTATTTACCTGTTCAAAAAAAGAAACGTAGTCAAATAAAAAAGTTTGGCGCTTTAAATTCTCCTTACACATTCATTTAGATAGGAGCGAATAAATGTGTAATGAATACTTTTAAAGAAACCCTCTTTTCATTAAAGAAAAGTAATTATTTGTTGAATGAGAGTGCTCAAATTCTCAATGATTGTGTAGTAAATAACAATGAAATCGATGAACGCTTACGCAATTCGATTAAATTTGCCCTTGGCATTATTAATTTAGCTAGTTACTGTCTTAAAAACGAACAAGAACAGCTAGACCATGAACTCAACCATGGTAATGAAGCGCCCTGGGAGTTTAGTTTCAATGAAGCTGAACAACTAATAGAATGCACTGTCAAAAATAACTTTGGCAACGAAAAACTAGTGGATTTAATTTTCCACATTGGTGATGCCATGGAGACTTACCGTACCACTAATATTAAGTTTAGAGTACCCAAGTCTTACTATGATGCCAAACAGCAAATTAGAAAGGTAATTAAAAACTAAGGACTATGATTGCGTCAATTTTTGGAAAAATTACCTTTGTTGGTAAGCGAAAAATTATTGTTGAAGCCAATTGTATTTCCTATTGGTTTAATGTGAAAGAAAACCATAGCTTCGAAAAGAACTTGGAAAAACCGCGCCAGGTCTTCTGTCAGATCATTAAAAGAATGGTTACGAACCAGATCTTGGAAGAGGGCTTTGCCTTCAATACCTTAGAGGAAAAGGAATGGTTTTCGAAGTTCATCGAACTCAATGGCATTGGTAGTAAAACTGCTTTAAACTTGCTCAATAACAACCTCGAGGAAATGCGTGATTATATTAAAAACAGTAACTACCATGCCTTGACTAAGTTAACTGGTTCTAACAGTAAGGTAGCTCGCGCATTGCTGGCACTGGAACTGTATGACCGAGACGATGGTGGTAAACGGATTAAACCCAACACTGCCATGGCCAATGACTATGATGAGATGTTTGACACCTTAAAGTCGTTGGGCTATAAACCCCAAGATATCCAAAACGCTTTGAGCAAAATTGAAATTAAACCTAACTTTGATGTAAGTGAAGTGATTGCTGAAGTGATTAAGTTAATGTCTTTCCAGAACAATGAAGTTACAAATAAAACCGCCTAACAATTTCGCTGAGTTTGTCGGTAAACAAGAAATTATTAACCAAATTCAACTCAGCATTAAAGCGTCCCGTATCAATAAGGCGCAGTTGGACCACATTCTTTTATACGGTCCACCTGGCGTTGGTAAAACCACATTAGCTCGCTTAATTGCGAGTGAAATGAACACCAAACTGCAAATTATTCAGGGTGGGCACTTACAGCGACCGAGTGATTTTCTCAACGCCGTTTCCCTCATTAAAAAGGGTGATGTACTGTTTGTGGATGAAATCCATGCAGTAGCACCCAGTGTGATGGAGTTAATGTTTCCCGTGATGGATGATTTTCGCGTCCAAGTCTTAATTGGCAAGGACTTTAATTCCAAGATGGTGGAAATGAAAGTTAATCCGTTTACCTTAATCGGAGCCACCACTCAGTTCGGTAAGATTATCAACCCGCTGGAAGATCGCTTTGGGATGATCCTTAACATTGACTACTACAGTAACCAAGAGATCGAGCGGATTGTAAGCATCTATGGTGAGCAAATGGAGTTGGAATTAAAACCGGAAGAAATTACCCAGATTACCCAACACAGTAAGCAAACCCCGCGGATTGCCATTCGAATTGTCAAGCGTTTGTTTGAACAGAAAATAGTCAACAAAAAGATTGATCTGGCTGCTCTGTTCAAAAGCTTAATGATTTACAAAAATGGTTTACAGAGCATTGATGTGCAATATCTCAAAGCACTCAACGGGCAGTACGAGCCTCAGGGTATTAAGTCAATTTGTTCGATGCTAGGGATTGATAAATCCACTGTCGAAAACAAGATTGAGCCCTTCTTGCTGCGGGAAAACATGATCCAAAAGACCAAGAAAGGGCGGATCATAACCCGCACTGGGCGTAATTATTTAACTAGCTGCTAACTTTTTGGCACGCTGGATAATCTCATAACCAAAGCTTTGGTTAATCTCATAGATCATTTGATCTAAAGCAAAGTGAGCAGTCTGTTGTTTAGGCTTAGCTTGCTGATACTCATAAAAAAGTAACCCTTCTGGTCTTGAACTGGAGGGGTTATTTTTTAATCCAAAAAAACTCACGCCAATTAAGCGGATGTTAAGCCCCACACCCATTAATAGCCTGTTAAAGAGCTTTTTGACTATTACGAGTAGGGTTTGGTAATCAGCGGTGTATTTCTTGATCGATTGAGAGTGGGAGTTGACAATAAAGTCATTGCTCTTTAACTGCACCACTACTCCCTTGCACATTTGAAAGGACAGTTGTAACCTAGCATACAACTCGTTGAAAATACTAGTGAGTTTTTGCTGGAGTTGATTGGAAGAGTAGTTCAAATCTTCCAGCGTTTCACTTACAGCAATTGAGCGACTTTTGACATCCTGCTCGCTTTGCGTATAAGTTTCACCCAAAGCCACAGCGTGCAAGCTTTCCCACGCAATACCAAACACCCGTTTGAACAAGGTCACATCTTTACACTTAGCGAGGTCTCCAATCTTGTGAAAATTATTTTTAAAGGCGTTGTCAAGTTGTCGTTTGCCTATCCCAGGAAGTTCCACAATTGGTAAGGGTCAGAGTTTGCGTTTAACTTCCTTGCTAAAACAGCTTTTAACCCCAAACGGTTTAGCCTGATTGGAAAAGATTTTCGCTAGGGTAAATTGGTTGGAAATACCAATGGAGATGTGAAAGTTTAACTGTTCATAAACGTGGCGCTGAATCCGTTTAGCCAAACTAAATGCTTTACGGGGTGAAAGGTCACGAAAACACACAAAGCCCTCATCAATCGAAAGGGTGTGTACCTCTAGGTTAAACTGCTCAGCAATCATGGCAAAGATCTTAGCGGAATACTTGCGGTAGTTACGAAAGTGTGATGTGGCAAAGATCGCTTGGGGACACAATTCCAAGGCCTTAGCAATTGGCATGCCCGACTTAATGCCATAGCTCCGGGCAAGGTAATTACAAGTCGACACCACACTCCTAGATGTCCGGTTACCGACAATTAAAGGTTGATTTTTGAGCTCGGGGTTTTCTATCTCTTCTACTGATGCAAAAAAGGCATCAAAATCAAAATAGAAAAAAAGCAGATTAGGATCAGCTTGTATGGTTGGATCTAAATATGTAAAAGTGCCGATCATGTTAATTTAGTTATGTTATTATTAACCTGCAATAAACCAAAGAAAATAACGAGTTTTTAACTTTAAAAAAGTTATCGAGGATTATCATCGTTTGTGCTATTGCAAAAATAATTTCTGATGGAAGCTAAAAAAGATAAAGCTCAGCAGGTTGCTGATGTCTCCCATTTGCTTTCAACCAGTGCAGGATTTGTCATTTTTGACTATACCAGCATGTCAGCAATTGAAGCGACTTCTATTCGCAAGAAGTTGTTTAAGAATGGCAGCAAAATTAAGGTAATTAAAAACAATATTTTGCGCCGTGCTTTAAAAGCTGGTAAGTTTGAAGGAATTGACGAAACTGCCATTAAAGGCAAATTGGCGGTTGCTGTTGGAGTGAACGAAATTGTAGAAACGTTGAAAGCTGTTGACGGTGTGGTAAAGGCGAAGGAAGCGATGAACTTTGTTTGTGGTTACTTTGACAACCGTGCTTTTAACAGTGCTGATTTAGAGAAGATTGCAAAACTGCCAGGACGAAACGAACTTTATGGGATGTTTCTCTCGGTATTACAAGCTCCATTGCGCAAGTTCTTATACGCTTTGGAAGCAGTTAAGGCTGCAAAATAATAAACCCCTCATAAATAATTAATAAGTAAATATAAAAATGGCAAAACTAGATAAAAACCAATTAATTGAATCGTTGAAGGAAATGACCATCATGGAAATCGATGAAATCATTAAGGCTGTAGAAGAAGCTTTTGGAGTATCGGCAACACCTGTAGTAGCTGCTGGTGCTGTTGGTGGTACACAAGAAGCTGCTAGCGAAGTGACTGTGAAAGTTACTGGTTACGCTGACAACGCTAAATTAGCTGTGTTAAAGCTTTACCGCGAAATTGCTGGTGTTGGTTTAATGGAAGCTAAAACTGCTGTGGAAAAACTTCCTTGTGTTGTTAAGCAAGACATCAAACCTGAAGAAGCTGAAGAACTTAAAAAGCGTTTCGTTGAAGTTGGAGCAACTGTTGAAATCAAATAATCAACAATGGCAGTACAACAACGACGCTCAAGCAAACACCGTCGTGACAAAAGACGTTCCCACGATGCACTAACAGCTCAAGCGCTGAGTGTTTGCCAAAAATGCGGTAAGAAAAAACTGTCCCACCGAGTCTGTTCGTGTGGAATGTATGGTGATCTGAGAGTAAAAAAGGCTTATTAAAAGCCTTTTTTATTTGCCTTCAACAGCTGTTACTTGTGTTTTATTTAAAACAGCAGCGTTGCGTGATTTTAAACGACGCGCACGATTAGTAGAAATAATACCTTTACGTGCTAAGCGATCTGCTTGGGAATAAACATTACCAAGGTTGTCTAGGTTAATTTCCTTGTGAAAATTCTTCACGTTGGTCTTTAACTTAGTTTTCTGTCCCTTGTTATTCAGATTACGTTTAATGTTTTGTCGTAAACGTTTTTCGTTCGATTTGATATTAGCCATTTAGGTTTTTTCGCGATTCACAGTATATTATTATCTTACTAATGTAGCTAACAATCATAAATGAATGGTGGTGGACAACAGGGTGGTTTTTTTGGCCTACTCGTCATAATTATCCCTGTCATATTGTTGATTGTCTTTTTCTCTAAAAAGAAGAATTCACAAAAAACAGAGTTTGGTGGTGAAGGGGGCAGCCGTGCTTCCAAAAAAGATGAAGTTTGAAAGACAGTAAAGCAATTTCTCCAAGAACAAAACGAACGCGGCAAGGAAATTATCAAAACTTTTGTCGCTAAGAATCCTAATCCCTTGCACTCCAGGAAAGACCGGCAGTTTTTTAACCAAGAAGTGCAAGCTTACATTACAGCCCATAACTTAAGCAAAACAGCAGCCAAGCGTTACCGTCACGAGCAATTAAAACTCAAACAACGTGAACTGTACTGCATTTACTTCATTACCAAGGACGCTAAAACCAGCGTTTTTGATGAAGCACGCATTATTGAAGCTGAAGTTTACCAAAAGCCCAATAAAACGGGTAAGGGTGCACCAGAACGTTTAATTCGAATCTTAGGATTGAAAAACTTCAATGACGAAATGAAGTGGATTCAACCACTAATGGATCGCGAGGAAAAACGAAAGGAAAAAGAAGAGAAACGCAAGCGGGAATTGGCAGCACGTCAGTTAAAAAGACAAGAAAAGAAAAAACAAAAAACTTCCAAATAACAAGATGATTAAAGTGGTTTTCTTTGGGACATCCACCCTTTCAAAGTGTTGTTTAGAAGCAATTTTTCAAGATCCTGAGTTTGTGGTTTGCGGCGTAGTGACCCAGCCCGATAAGGTAAACGAGCGCAACAATAAAATTAACTTTTCTGCAGTTAAACAATTTTGTATAGAAAACAACATTCCCTGTTTTCAACCAGAAAAAAACATTCAGATTAAAACAGAGTTAGCCCAACTCCAAGCTGATATTGGCGTTTGTGTAGCCTTTGGCCAATACATCCACAACGATATTATTAACCTCTTTCCTTACAAGATTGCCAACCTACACCCCTCAAAATTACCACTACTGCGTGGTGGAGCACCATTGCACTGAACCATTATTAACGGTTTTACGACCTCTAGTTTAAGTGTGATTGAACTCGTACAAAAAATGGATTCTGGTCCGATTTGAAAGCAAAAGGACTTTAAGGTGAATCCCAATTGGAATACCGGTGATCTATTTGAATACGTGCAAACTCACGCACCGCAATTTCTGATCCAGTGCCTTAAGGAAATTGTAAGTGGTAAGAGTCAATGAAAAGAGCAAATTAATCCACCTACTTTTGGTTTGAATATTAAAAAGGAACAAGAAAGACTTGACCTCAATTTGGAACCAAAAGCATTCATCAATTGGGTGAAAGGGTTGGCACCTAAACCTGGTGGTTGACTGGAATTTGAAGGACAAAACATTAAAATTCTCCAAGCAACTTACTTGGGCAAAATGACAAGTACAACCGCAGTTGGTCAAATCACTAAAATTTCTAAACAAGGTATAGAAATCGCCTTAGCTAATGATGAAATTGTTCTACTCCAAATAATTCAAATTCCGGGAAAGCGGGCAATGGGAGTGTCAGAAATAATTAACGGTAAACATCCTTTTGCTGAAGGTAAATTTTTTCATTAATGCTCACCAAGGCCTTTTTTTTAAAAAACTTTGATCGCTCAAAGGAGTTGATTCCTCTGTCTTACGCTGATGTTTTTGGCGCTGCTAGTCAATTGTTAAAAAAACACCATAAGCAGGTCGATACCGAAATTGATGTGCAAGAAGATCTAATTGAAGATCCGGTATTTGAAATAGATATTTTAGAGCTACTCAACGAAGCGCCAGAACTATTATTTGATAGTAAAAATCCTCGGGTTAAAGAGGCACAAATCATTATTGAAAAGGCCAAAAAAGATATTGCTAGCTATTTCCACTTAGACAATATTTTAGACACTGACAACTTAGGGTTAAAAGCCACTGTTACCGAAAAAATCCAATTTACCGAAAAACAAATCGAAGCTGCAGTACAAAACAAAAAAGCAGCGATTATTTTTAAACCAGTGTTTACCGTCAACCAGTGTTTAATCCAACCAGATGCTGTAGTAGTACATGCTAATGGTTTGTGTGAGTTTGTTGTTATTAAAGCGACAACAAATACCAAGAGAAAATTTATCTTGGAAATTATTTATGACTTCTTGCTGTTTGAAAAATTGGGCAAATACAAACTGGTTAACTATTACTTCTGCATAGTTAATTATGAACTGAAAAATAAACACAATGTTTCCTTCTTTTTAAACACCGAAATTAAAACAGCCAAAAACAGTAGTACGAGTAAAACTAAAGAAGAGCAAGTGTTGTACGGGCATCTCCCTTTTAATGATCCAAAAAAAATTGCTTACATTCATAGCAAGAAGAGTGGTGGTGTCAATGGTTTTCTGCTCGTCAAACTAGTGGACAACATTATTAGAAGCGGTGTTACTAATTTGGAACAAATTATTAGTTTTGTTTTTCGTGAACTGAATGCACCCAGCATTCGTAGCTTAAAGCAAATTATTAGTGAAGTAGCTAAAGTACAACTTGATTTTTGGAACATTATTGATGATGTTAAACAACACCAAGAGTTACAAGACAACCAAATTACCTTCAATTACAGTGATGCCTTCAACTCTTTTTGGAATAACTATTTATTGCGCAATCTAATTAAATTAGTCTTTGCTTATAAATACAGTGAAATCTTTCGACTTTCAGGAAAATTAGCTAAATGAGATGAAGTAGTTGAGGCTTATAAAGAAAACAAATCGGTGAAAATTGATGGCTTTTTATACGAACTTAACCAAAGGAAAATGAAAAAAACTAAAAATCCCGCTACTTCCCAATTTAACAAAATCCACTTTTTTCTAAGAGCTTGAAACGATAAAAAGGGGATTGCTGTTGGTAATAAATTTAAGAGTGTTTGACAAAAGCTCAAAGAAAAAAAAGTTTACTTTGATTTTGAAACAATTAGTTCTGCAGTTAGAGTAATTGATAAATCCTTACCCTTTACCCAAATTGTGACCCAGTGTTCTCTTATTGTTGATGACAATACCGAGTCTGATAAAAGCAAGTTAGTTTGTCAAAACCTGATCTTTGATCCGCTAACTATTGGTATTGAGGACTTTAAAACAGTCGTTGATGTCCTTTACCAAAAGCAGTGTGACCAGTACAGTTTTGTCGTTTATAACAAATCGTTTGAAAAGAACAGGTTACTGGAAATGGCTACTTTTATCAATGAAGCTCCATACCAACAAAGAGTGCAAGCAATTATTGAAAATTTATTTGATTTAGCAGATATTTTTGGACTTGAAAATGATTGCTTAGCCTTTAAACAACTTGATGGTTTTTCATCAATTAAAAAGGTCTTGCCAATGATTGATCAACGCTTTTTAGATGCTAGTCGAACTGTTAGTTACCAATCATTAAAGGTACAAAAAGGTGATGTAGCACAAGAGCTTACTTTGGCACGCTTTTTAAATTGTTTAGACGAACAGCAATGAGCACAAACTGCGTTAGAATTAAAGCAGTACTGTGAAAACGATGTACGTGCCATGATTGCAATCGAACTGTTTATTAAGGACTTGATTACGAATCAGCTTTAACCTTATTCTTTAAAGCTTTTAGTTTTGGTTTACGTGCCTTTCAGTTAATCTTTTTCGGTCTAACTGTTAAAGCTCGCATAGGCTTTTGACTGTCTAACTTATTTTTAATGTCACTAAAAAAGTTAATGCCTTTACCACTTTTAAGAAACTGGGTAAAGGCTGACATTGCATTGTGTTCTAACACGCTCTTGTCATCATAGTGTTTACTGTTAGGTACAGCTTGTGACCAGATAATGTCACCGTTAAGCTCAATAATACTCAACACAAGGTTATTGCTAGTTAAACTAACCCGTACCCTAGGTTCTGGTAGCTTTTGTTCTTGAAACAGTGAAAAGAAGTTGTACTTGAGAATTTCTGAGTAAGTTTTTTTAAAAATATGTTCTTCGACAAATTGATTCGCTTTCTCTATGCCAAGGTCTTCGTAAATGGCTGCCACTAACGCCTCTAGTACATCACCAAAAAGTGTTTCAGTATACGGCATTCCTTTACCTAACTTAATGAAATTGCCAAAGTTCAATTCCTTCCCAATACGGTTGAGGTTTTCGCCCTTAACAATTTCAATCTTAGTTCTGGTCAACATACCTTCGTTGTAATTGGGATAAAGCTCAAACAGTTTTTTCGCTACTATGAAATCAATTAAGGCATCACCTAAAAATTCCAATCGATCATAGCTTGCGCGTGCATCTTCATTTTCATTAACGTAAGAAGAATGAACAAAGGCATCTTCGTAAAACTGTCAGTTTTGCGGTTCAATGTTTAAACTCTTGAAAAAAGCGACAAACTTTTCATCAATGACCTTAGGTTTTTGAGTTTTATCTTTCTTATTTTTCATAGTTATCTAAGGAACTTTTAATGGCGTTAATTAGATCAGACTTTAAGCTAGTGTGTGCTAATCTAATGGTGCTTAAAAACTGTTGTTTATCAGCTGATCCATGGGTTTTTAAAGCAAGTTTATTTAAACCGATAACAACGGCGCCTGCGTTATTTTTGTAATCAAAACGCTTAGCAATTCTTTTCAAAATTCCCAAACTAAAAAGCCCAGCCAACGGGTTACGCTTATAACCTTGCTTGAGAAGACGCGCAATTGTTTTAAAGGTGCCTTCCATTGATTTCAACACAAGATTACCGCTGTAACCATCAGCCACTAAGATATCACACACCCCGTCAAGTAAGAAGCGTGGTTCAATGAAACCAGTGAAGTTAAGGTGCTTATCTTCGTTTAGCAATTGGTAGCCTTCCTGGTGGTAATCAAAACCTTTATTGATTTCGGTACCAATGTTTAGCAATGCTATACGTGGACTAATTTTGTTGGTGGTCTTTTTGACAAAAATATCCGCCATTAGGCCCAAAAAATAGAGCTCTTTACCCGTAAAGTTCTTGTTAGCGCCAACATCTAAAAAGTAAAACCAGTCGTTATTGGCAGTTGGTACATAAGACATAAAAGCACTTTTAACATCTTTATGGATTTTGCCAAAGCCATCGTATGTTAAAGAGGCATAAACGGCCGAGGAGCCAGCGGAAATCACAACATCAGCAGCACCATCACGCACAAGGTTAATCGCTTTCTGCATTGAGTTGTTAACCTTGCGCCGTGCGGTTAGTGGTGTGTCGGTCATATCTATGCAAGAGCTGGCTAATTGCTTGGTAATGTTTTTAGGTAGATAATCAAGTCCGTCAAAGGTCTTTTCATCACCAACTAAGATAAATTCCAACTCTTGATGATAGCTCCAGTATTCTAATACTGCGTCAATTGCTTCACGCGGGTGGTTTTCAAAACCTAAACAATCAACTGCTAGTCTAAAAGCCATTATTGTATGCCTAGTATATAAGAAAAAACTTTTTGTTCCCCTGGGGAAAACTCACAGAAAAGCTTGTATTTCTTTTCTATTTTTTCCTTCATTGCTTCCGCTTCCTCAGCGGTGATATCTTTACCATAGTAAGCTAAAAGAAACTCCGGCTTTTTTACCTTTTTCAAAAGGTGGTCAATGGTGTTGAACACGCATTGAACAAGCTCTTTTTCACTAACACAAATGCTGTTATTAGCTACAGCAATAAAGTCACCCTTATTAACTTCAATCCGATTTTCCTTGTACTTTTTCGAAGCTTGAGTAATGGTTGCAGATGCAAAGCCTTTGACAAAACGGCGCATTGTTTTGACATTGGTGTGAATGTTAAGGTCACTGTTAAAAACAGTAAGCGCCGCTAGTGATTCAATCGGGTTGGTAGTCATCACACAATCAGCACTAATCTTTTGGTGCTTTAATTGTTTTAACGCTTCGTTGGCAGAGAGAAAGTAGTTCTTGTCATGTAACAAGAAAATAATGTTACTGGAATGAGTTAGCTTGACTGCTTCTAGTAACGAAAAGACCGAAGGTGCACCGGTGTCATCGGTACACAAGATAGCTTGAATGTCATAGTCTTCGCGAATCCGATCAGCAAAAGCTTCAGTTGGTACAGTCACTACTATGGCTGGTTTAATGTTGCGCTTCACAGGAGCGGGTTTTTGCTTCGCTACCTGTAAATTCATGTTTTCAATTTTGACGAGTTCAAACTCACCATAGTTAAGCCCAAGTTCTAATAACAGGTTAGGTTTTTCGGTATGGGCGTGTACTTTAACAAAGCCGTTATCTTTATCACTAGCAACTACTACCGAAGTGGCAATTTTGCTCACCTTACTTTCAAACTTCTTTTGGTTAAACTTCTGCTTTTCAACTTCTTGACTTACTGACATGCCCAGTTTCAAGACATATTCAGTGCAGTAACCAAACTCTTCTTCCGTAACGTGTTTTTCACCGCTAATGGTCATTTGGCTCAATTCAGCACTGGTGAGCTTACCATCGTTTAAAGTAGCCTTTTCACCATAAAAGGACAACATCCCCTCTAAAAAGCAAGCGAACCCATAAGCACCTGAATCTACTACACCAGAGGCCTTTAACACTGGGAGCATTTGCGGAGTTTTTTTAACTGTTTGTCAGGCTGTTTTAATTACTTGTTCAAACAGTTTTTGCACTGTTTTGGCACGGTTCTTTTGGTTTTTAAAGTCTGTGCTAATTAAGCGAATAACCGTGAGCATCGTACCCTCTACGGGCTTGGAGACATTCTTGTATGCCACCTCTTCGGCCTTTATAAAAGCTGTAGCAAAATCCTCTATGCCAAGTTCGGTTTCGGTGGCTGTTTTAGTGCTAATCATGTCACTAAAAAAGCCCTTCATAATTTGGGAAAAAATAACACCAGAGTTACCGCGTGAGAAAAGTAAAAGGTCACGGGTAAAGCTTTTGCCCAAATCACTAAAGCTCTTAATGTGGCTAATTTCACTCTCTAGTTTTTTGAACGCTTCACTCAACGTGACCTTCATGTTTGTCCCCGTATCACCATCTGGCACCGGGAAAACATTGAGTTGATTAATGTATTCGAAATTATTAGCTATATTCTTACAACCAAGACGCAACATGTCAATAAAGTTGCTTAAATTTACTGTAGACATACGAAGTTAAAACCTGTTTTATCTGTGATAAATCGGTTTATATTGCCATTAATTTTAAATTGAAACCCCTTTAATTAAACTAGCAAATCACGGTACTAGTTGGCTTTTAAACTGCCGTTGCGAGTAATTAGCCAGTAAGGGAAATTTCTGCTTATCTAATTGGGCAAAACTTAGATTTGTGGCATGTAAGGCTTGGTGTTTAAAATCGCGGTTACGGTTTTTAAATTGCAGTAATTGGTATTTTCTATCACCAACTAACTGGGTATTCAAGAGGTTTAAGCAAGCACGAATTTGGTGGGTTTTACCACTTACTAAGCGTATAGTAAGCAACGAAAGATCTAACTTAGTTAAGTACTTACTGTTTTCAAAAATGGTGGTAATCGGTTTAGCGTTAGGAAATGGTTTGGCCTTAACTGTTACCAAAGCTTGATAAGCATCTTTCGCTCAATAAGCTTTTTGTGTGCCCTTAAAATTAAATGGGCCAAACACTAATCCTTGGTATTGTTTCATTAACAAGTTATGGCGAAAAACCGCCTCTAACTCCTGTAATGCCTGGTTGGTTTTAGCACCTATTACCAAACCACAGGTATTACGATCAATTCGGTGCGCAAACCGTGGTATGAAGCTAAGACTGTTTTTAAAGTATTGATTATAACCACAGTGTTTCAGTAAGCTGTTAGCTAAATTAAACAGTTCGTGTTTAGCATCGGGTTGACACACTACACCCGCTGGTTTGTCCACCACAATGAGCTGTTCATCCTCAAAGATTACCTGCAAATCATCTTGGACTTGGGTTAAATCCACTACTTCGCTGTGGTTGTGTAAGTAAGGTTCAATTGCAAACTGAAAAACGATTGTATCACCGCTGCTAACACGCTGACCTAATTTAGCCTTCGTGTTATTGAGCAAGACCTTACCTAAGCGCAACAATTTAAAGAACTGACTTAGCTTAATGTTGGGCAACAACTTTAAACAAAACTTTTCAATCCTAACGTTTTCAACAGCTTTAGGCACTACAAACTTAGCTGTTGACATGCTTTTGTTTGGCTAAAAAGTCATCCATAATTTCCACTAACTTCATAAACTGACGCTTGTCATCCAACACAAAGGCAGCAGCATACTTGTGCCCACCACCACCAAACATTTGCGCAAAGTTATTAATCGGAATTGAGCGTGAACGAATGGAACCAATTCACTTCTTAATGTCTTCGTTAAAGTAACAAGTCGTCCAAATTTTGACTCCTTTGATGTTGTTTAAAGCGTGCACCATCGGTAGTGGTGAGACCACCCCAAACTGCTTATAAGTCCCTTTTTTAAGAAGTGCGTAAGCCAAACCATTCGGGGTGATCTTGGCTTTGTTTAAGACATAGCTAAAGTACTTGTGTTCTAAAAGTGGTTTTAGATAAACTGCATCATGAACCTTATTGCGGTTAAATCCAGTTTCTAGTAACTTAGCAGTTAAAGCAAAGGTTTGTGGGGTGGTAGCGGAACTGGAAAAGCGCTGTGTATCGGTAATAATACCCGCATAAATGTAAGCAGCCATCTCAGCATTAAGTTCATAACCCATTTGCAAGATTAAGTAACCGACCATTTCTGCTGCTGCTGAAAAAGCCGGGTCAATCCATTCCAGATCAGCAAAACTCTCGGTTTTAGGATGGTGATCAATCCGCACGGTTTCTTTAGCTAATTTATGTTTTTGGGTTAATACTCGTTCTTGGTTGGAGGTGTCAAAGATAATTGCCAATGAGTTTTTTACAAACGCATCATCAATAGGTGCCGCTTCAAAGGGGAACAAATCTTTCCCGTCAGCCTTAATGTTGTGTGATCCCATCACGTAAGCCTTTTTATGGGGGAAGTAAACCGCTAAAAAGGCCTTAAAGGCAAAAGCAGCGCCAAAGGCATCAAAGTCCGGGTTTACATGCACATATAACGAAAAGTTATCGAATGTCTTGAGTTTATTAGTTAGGTTATGAATAAAATGGGGATCGATGTTAATCATTTTGCAGCTTCTTGACACCAGCTTCAATTAGTTCACGGACAAACAAAAGTTCACTCTCAAGCTTTTCAACGATCGCTAACTTAGCCTTAGTAGTTGGTTTTTTGGGAGCAAACACAGTACAGGTATCCATATGGTGCTCAATTGAAGTTTCAAAGGTACCCAGTGCCTTAGCTCATTCAATTATTTTATCTTTACTTAAGCCTATTAACGGGCGAATCACAAAGGTATTGGGCACAACAGCTTGGATTACCTTCAAATTATCAATTGTTTGTGAAGCTACTTGTCCCAATGCTTCCCCGGTGATTAAACAATCATATTTAAACAAACTAGCAATCTTGTAAAAGCAACGGCGCATTAAAACAATCCGGTATCCTTCCAGACTAATATGACTCAACTCTTTCTGTAAATCGGTAAAGTCAAAGACAAACAGTCTCCCAGTACAAAGCGTTTGGTTAAGGGAAACTAAGTTAGCTAACCGTTGAATTTTGCCTATTGTGGCAGCGTTATGTCCGGGCTCATTAATAAAAGTAATAAAGTCAACATTAAAACCTCGTTGTAAAACTAGGGAAGCGGCTACTGGACTGTCTATGCCACCAGACAGTAACGCCAAAGCAGTACCACTAGTAAAGACCGGTAGACCACCAATACCATTAAAGCTTTCACTAATTATTAGGAAATGTTCCTTAGTTACTTCGACATGCACCGTAATGGCAGGGTCAACTAACTTACCCTTCAATTGGTATTTTTCCCATAACTGTAAAGCTAGGTACTTTTTGAAGGCACTGGAATTGCAAGCAAATAATTTATCACGCCGTCTTACCTCAATTTTGAACGATGGGTGGGATTGTACCACTTGCTGTAGCAGTGCTAACAATTGCGCTTCATCATGAGGGATTTGTAAGGCAAACAAGCAAACACTCAAACCCGGTAATCTGGTAAAGAGATTTTTTAATAACGATCTTTGGGTGGAAATTACTTCACCCACTACCAACATGTCAAACTGGTAGTCAACTGGGTTAGTAATGCTTAGTTTCTTAAAGGCTTGTTTAACGTTCTTTTTGAGTTGTTTGACAAACTGCAAGCGGTTCTTGCCCTTTAAAACAAGTTCGCCATAACGGCAGATTAGAATAGTATTTGGTTCATTATGCAATCCCATTTTCCCTCAGTCACTCCCAGTCTTGTGAGGTTAATTGTGGACTTTTACCAGTACGAATAAAACTGTTGTACCACAGCTGTGCTTTATTAGTTAAAACTTCAAAGGCCTTGCGATCAATTTGTACTGGTACCAAACGGATGTCGCGTTGCTTTGCTTCAAACCATTCGGGATGCACATAGTCTTGTGGTTCTAAAAAAGCAATCGCAAACATTCCTTTGGTAATATTGCGCAAGTACAAATACAACCCAATTTGGCAATAGTAAGCCGGGCTAATCACGATTTTGCCGTTAATGTCAAACCAACTATCTTTCTTTCCGTTAATCTTTTTGACAATCGGCATGCCGTTTTCATCAAACACAATTTTGAGGTTACCGTTAATCTTTTTATAGACCAAAGAATCACAGGAAGTGGTTTTAATTTCTAACATCGGCGCATTGGTATCGTAAGCCAATTTTCCATAAACATCGACCGGTTCCCCATCAGGAATTCCTCCAAAAACTGGATTTTCAGGAAAGAGATCCCACTTAACCTCCTTGGGATCATACGAATGGAAATTAAAACCAGTTTTAGCTATCACATAATCACGGATTTTGGGTTCAATAACTTGCCCCGCGCGTGAGAGTGTTTCATCAAATTCATCCTCATAAATCTTGACCATGTTAGCTCAAGTTTTAAGTGAAGTTTCATACTCAGAAATACCTATTACTTTACCAAATCGGGTACCGGTAATCTTGCGAAAACTACCTTTATGTTTTTGAAAATAGTTGTTTGACAAAACTATTTGGTTGTCAATAATATCAAAATCGATTTTGTACTGTTTGGTAAATCCCATAAATCATTACAAGAGTTGTGCACAAAGCTTTTGTAACACATCAGGAATGTCCTGCACTGTTAAACCTTCATTGTTCTGTTTCTTGATGAAAACAGCACTAGCTAACTGCTCACTGTTTTGCACCAAAGTTTCATCTAAGTTAATGTAGTGCACATCTTTAAGCATTAAGCCTTGGGTCTTAGTGTATTTTTCCAGTGCATTAAATTTAGTTACTTTATTACTTGTAAACAACAGGTTATTGGGATAGAAATTGCTGTGGGTAAAGCGTGGTTGACCACTAGCAATTTGGTCAATAAAAGCTAAGTCCATGTGGTATTGGGGACTAAAGACGTAGATAGAACTGAGTTCCAATTTACCGAGTTGTTTTAAAAAGTTTTCGTACTTTAAGGTTTGTTCAAAGTCCGTTAACCACAGCGACTTAAAGTCCTTGTACTTTTGTAGTGAAATGAAATAGTTAGCTGATAAATTAGCGGTATCATCAACGCGGCTTTTACCCATGGTAAAGATCCCGGAATTGGTAATCACAGCGTGGTGGATAATGACATTAATACTAGCGAGGTCTAAAAAGGCTTGATAGTCATACTGATTGGTTTTGCAAAACCAGGTAATGGCACCTGATTCCGCAATTACATCAACGTTGTTGAGTGAAAGTAAATCTAACAGATACACAATTGTTTGCTGTCAGCAACTCGAAATAAAAACGAGCTGGTGCTTTTCTTGTGCCTTATTGAAAAAATCCTTTCAAATTGCTAGGTCGTTAGCAGCGTCAAAGAAGTTAGCTGGATTAATATCTAGAGCAACAATCTTTCTATCCATCCTTACGCACTTTTTTGACAAAGTTAGCTCAATACAGTTTCTGTACCTTCTCACCTTTAAAGCCACGACAAGTTATTCGAGTAAGATCCTGCATCTCCTTATCGCCGACAATAATTTGCAGTGGAATCTTGTTAACAATGGCTGTGCGAACTTTTTTGCCCAAACGATCTTCGCTCTCATCTAGGCTGACCCTAATGTTTTCCTTTAACAATTTTTGGTACAGTTTTTTGGCAGCCTTTTCGTGTTTATCGATGTTAACCGGAATCACCACTACTTGTACTGGTGCCATTCACAACGGTAGATTGCCATGAGTTTTTTCCAATAAAGCAGCAATAAAACGCTCGTACGTACCGACTATGCCAACGTGAATAATTACGGGAGTTTGAGCATTGCTCTGCTCATCAAAGTAACGCAATTCAAAGCGTTCTGGCAGTAAAAAATCCAGTTGAATAGTGGCAATGGTAATCATCTTATTGAGTGCCGTTTTAAACTGAAAGTCAATTTTAGGTCCATAAAAAGCAGCTGCACCCACATCAATTTTATAGGGCACATTTAACTCCTTAAACACGCTTTCAATTTGGCTTTCTGAGTTAGCTCATAACTGATCGTTGTCAATGAATTTGGCCTTGTTATTAGGATCGTGTAAGGCCAAGTCAATTCGGTCAAAGCTAAAACCAAACTTGTTGTTGACCGTTTGGATTAACTTAAAGGCATTTTTAATCTCAGCTTTAATTTGGTCAGTACGACAAAAGATGTGGTTGTCGAGCAGTGTCATGCACCTTACCCGTTCCAAACCAGTAAGCCCACCCGATGCTTCAAAGCGGTGCAAGATGGAATCCTCACAGAAGCGTTGTGGTAACTCTTTGTAAGAGCGGCGTTTGTGCTTGAAAATCAAACAGTGGTGCGGACAGGTCATTGGCCGGAGCATCATCATTTGGTTTTCTAATTTGACAGAGGGGAACATGTCCTCACTATAGTGCACATAGTGACCACTAATCTTGTACAGCTCTAAGTTAGCTAATACTGGTGAAGATACCGTTTTAAAACCAAACATTAACTGTTGGTAGTGGACAAAATCACCAATAATCTTTTTTAAAACTGCTCCCTTTTCCAATCAAATCGGAAAGCCTGCACCGACTAAGGGATCAAAGGTAAAGATCTCTAAACGCTTACCAATAGCACGGTGATCATTTTGGTATTTTTGTTCATTAGCCGCAATAAGACTTTTAAGCTGTTCTTTAGAGGCTGCAAAAATTCCCACAAGCCTTTGTAGTTGTGGTTTTGTGGGATCACCTAAAAAATAGTTGGCACTAACATTGAGCAGTTTCACAAAGCCCTGTTTGGGTAATGGCAACACTAAATCATGCACCCAAAATGTTTGTTCATTTACGGTGGTAACTACAAGGCTGTTGCTTTCTATACTTTCAATAAGCGTGGTTGTAAACGGATCAGCTTGAAAAAATTTGAGTGATTTTGTCTTATCAATTTTTGTCTGAACAACATTATTTAACTGATTAAAATAAGCATTTAAGTCCTGTTCGAGCTGTTCAAACTCTTTAATGGAAAAGCTCTTTTCCACCTTAAAGTCAAGGTAAAACTCGCCTTCTTGTACCCCGTAACTACCAAACTGTACTTGAGGATAGTTAGCTTTTAATCAGCTTTGTAATAAAAGAACACCAGCTAGCATTAGATCTCTTTGTAATCGATAACTATTTCGTTTTTCGGATTGAAAATCTTGACAATTTCAATTGTGGTCTTTCAAATTTCACTCTTACGCTCTAAATAAGTGCGCAAAATGCCTTCAATACTAATGGTCTTGTACTTCTTGGTGTGTTTCTCCAATTCGTAAGCCAGTTGACCATTGGCATAAATGACGTAATAGTCAGTAAATAAACGCTCGCCAAAAAAGCGCATTTGCTTAATGGTTACTAAAAATCCAGTTTTTTTAACGCTTCAGCACGAACTTTCTATCTCACCCTCCAAAAAAACGCGGTTAAGCATATTAGTTTGGTAATTGTCCAGTTAAGTTAAAGTGAAATTTAAAGTGGTTGATGGTTTCTAACACCATGCGCTCTTCTAGCAAGGCTGTACGCACACCCTCAAACTTTTGTTTATCATCCAAGTATTCGCGCACACTTTGATTGGTTTTTTCGTAATAAAGACTAATTACATTCTTCACCACTTCATCAGTGATTTCCACTTTCCATTCTTTTTGGAGGTGGTTAAACACTAAAGCCTTCTTAATAATTTTTTCAGCAATTGCTTGCACGGTGTTGTCCTCAGCGCCCTGAATCACATCACGCTTTAGACCTTCCATGACATTCTTCAATTCAGCTTCATCGATGTTGAATTCAAAGTAACCCACAATAGCATTCATGGCACAGGAAAAGAGGTTGTCCTTAATGACAATGTGCGTTAGTTCATGATAAAGCGCACTTTCCTTAGCGTCCTTAAAGGAAGCTAAGATCCGTTGGCGGTGTTGTTCAATAAAAGCAGGGTCAGCAAAGATGCGTTCTACTTCAATTACCTTATCGTACTGAATTGGTTTAACTAGTTTAGCGGTTGATTTAAGATTTGTAGCCATATTTAATATCGTTTAAATTGTAATTCTTAAACCAACGCCCTTTTACATTTGTTGTGGTGCAGTAATGTTTAAGATTTGCAATAGCTGACCAAAAACATGGTGCAATGATTGCAGTAAAAGTAAGCGTTGCTGGGTTAAATTGTACTGCGCTGGATCGTTAATTTTGCAAGCCTTATACCAAGAATGAAACAAGGTAGCTGTCTCGTATAAGTAATTAGTTAAGAGGTGCAGTTCATGGCTAACCTGAACTTTTTCCAGTAAGCTCTTAAAGTACACTAACTGGTCTAGTAAAGCAATTTCCTTTTCATGCGTTAATAAGCTGCAGTCATTTACTTGAGCTAGTGGTGCTTGATCCACTACCTTTAATACACTGCACATTCTGGCATACGCATATTGCACGTAATAAACCGGATTGGAAGAGTTTTGCAGGTTAGCAGTGTCCAAATCAATCTTGATAATCGAGTTGTTGGTTTGCGACAACATAAACCAACGCGCCGCATCCTCACTGAGCATTTGCAACATTTGCTTAATGGTAATAACGTTGCCAGCACGTTTGGACAATCTGACTATTTCCTTATTCTTGTACAGCATTACCAACTGTAAGGCAGTAATTTTGAGCTGGCATTTTTGGTGAATCTTTAGTCCCTCATAAACACAGTGCATCCGTTTAATGTGCCCTTCATGATCACTGCCCCAAATGTCGTATAAGAGGTCAAAGCCCCGCGCAGCCTTGTCTAAGTGGTAAGCGACATCGGTACCAAAGTAAGAAGGCCGATTGTCTTGACGCACTAACACCCGGTCCTTATCATCACCATAGAGCGTAGTCTTCAATCAAATGGCACCATCCTTTTCATAGTGCGCTTCGTCCTTAAACTGGGTTAACAGTTTTTTGTAATCTGTTTCCTTAACCACTTGACTTTCAAACTTCCAGCTGTCAAAGTGAATGTGGATTGTAGCTAAGTCTTGCTTAATTACTTCCAAGACTTTGGCAGTACAGTCAGCTAGTAACTGGTCCAGAAAGGGTTGTTGTAAAAACTCTTCAAAGCTGATGTTATCTAAAGGAATACCGGTAATAGTTTTGGCAATACTGTCCACTAGCTCCCCCTCATAACCATCAGGGTGAGGAGTGACCTTAATGTTTTGTTGTAAGTGGAGTGCTTGGTACACACTAAAACCAAACACCCGTGCTTGTTGACCATAGTCATTGACCCAGTATTCCCGAACTGTTTGAAAGCCTAAAAAAACCATCAGGTTATTGAGTACATGACCAAAGAAGGCATTGCGGACATGACCTAAGTGCAACTCACCCGTGGGGTTAGCCGACACCCATTCAATTAGAACCTTAGTTGGTTTAGGGGCAAAAGTAGGTTTGGTTTTTAAAAAATCGAGGTATTTTTGAGGGCTAATTTCAAAGTTAATGAAGTTGTTATTAGCGACAAACACACGGCGGTAATGCGCCTTTTTGTGTTTTAACAACCAAGCAGCAATCTGTTGGGCGGTTTCCTTTTGATTTACACCCTTTAAGTGGAACACAACGGTGGAAGCAAAACCACTAAAGCTGTTGTTTTTAACAAGTTTGACTAACTCTTTGTGCTCATCAAATTCTAGATTCAAAGCGTTAAGACATTCTTGAAGATCTGTGTTAATGAAAAGCATTACTTAAATTATATTTAACCTTGCAAAATGTCTTTTACACTAACAGTTATTGGTGGCGGTCATGCCGGATTAGAGGCCGCATTTATTGCTAGTAAACTAGGGCTAAAAGTTAACCTCTTAGTACTAGATCCCAACCATGTGGGGTCGTGTCCCTGTAACCCGGCCATAGGAGGTCCTGCTAAAGGTATAGTAACCCGGGAAATCGATGTTTTAGGAGGGATGCAGGGTAAGGCCGCTGATGCCACTGCTTTACAATACAAGCTGCTCAACAGCTCGAAAGGTCCAGCAGTGCAAGCAATCCGCGCTCAAATAGACAAAATTGCTTATCAGAAGTGGTTTCGCCAGCAAATTGACCAAACGCCTAACATTGAGTTAATTGCTGGGGAAGCCGTTGATATCCTTGAAAGTAACGGTAAAGTTAAAGGTGTCGTGTTAGCTGATGGTAGTGAACTGGCATCAGATGCTGTTATTGTGACTACGGGAACTTATCTAAAGGCCAAAACTTACTGTGGTAGCTTAAGCAAAGAAGAAGGTCCTGACCGCGCTAAGCGCAGTGAATACCTTTCCACTAACTTAATTAAGCGGGGTTTTAAAACGTTACGACTAAAAACTGGGACACCACCACGGATCCTACGCGAATCACTCGACTTTAGCCAAATGGCTGTGGAAGCTAACACTACCCCTCATTTAGCCTTTAGCTTTACTACTAAGAATTATTTACCACTAGAACAGCAAGTGATCTGTCACTTAATCCATACCAATCCCCAGATCCATCAGTTAATTCTGGCCAACTTAAAACAGTCCGCTGTCTTTAACGGTAGTATTAAAGCAAACGGACCACTTTACTGTCCGAGTATTGAAGACAAGGTATTTCGCTTTCAAGACAAAGAACGGCACCAAATCTTTGTCGAACCTGAATCACTTAGTTTAGAAACGGTTTACCTAGCAGGGTTTTCCACTTCCTTTCCCCCAGAGGTGCAAGAGCACATTGTCCGTTTACTCCCTGGGTTTAAAAATGCCCGGTTTCAAAAGTATGGCTATGCCATTGAATATGATGCCTTTAGTTCGATACAACTCAAATCAACACTAGAAACTAAGTTAATTCAAAATCTGTATTTTGCCGGACAGATTAATGGTACCAGTGGGTATGAGGAAGCAGCTGGCCAGGGCTTAATCGCTGGGATTAACGCCGCTTTAAAGCTACAGCGTAAACCCGAATTTGTGTTACAGCGCAATGAGGCATACCTTGGTGTCATGATTAACGACCTGGTTACTAAGGAAATTAGTGATCCCTACCGCTTGTTAACTTCGCGCGCTGAACACCGTTTGTGGTTGCGCAATGATAACTTACAAGAACGTCTAATTGAAAAGAGCCGAGCCTTAGGTTTAGTGGAAGCAGATGTGTATGCCAATTACTTGGAACAACAACAAAAGAAAAAACAATTAATTGATTATTTACAAACCACTACCGTTGGGCAAATTGCTGCCTTGAAATTAAACTTTAAAAACACGGCCCAAACGCTGTTTGACTTTACCAAGCGTGCTGAAATTAAGCTGGTTGATTTAGTACAACTACTCCCCAAGCGTTTTGACTTGGATGTACAAAGTTTAAACCAGATTGACATTGACATTAAGTACGCTGGTTACATTAAGAAGAGTGAAAAGTACTTCAAGAGTTTAAACAACCTCAGTAGTGTCAAAATTCCCTTAAAGTTAAATTACCACAAAGTACCTAACTTAGCGAGTGAAGCAATTGTTAAACTCTCCAAAATTAGACCAACTGATCTCAGTGTAGCTAGTCAAGTAGCGGGCATTAACTTTAACGATATTTTAGCGATTAAACATTTTTTAGATAACCATGAATAACAGTAAATTGAAACAGTATGTTCAACTAGTACAAACAGCCAACCAAAACTTTAACCTCACCGGTCTTAAAACTGAAGGCGAAATTTACGAACACTTAGTCCAAGAAATTATCGAGCTCTTTAATGAGTATGACAGTTACTTTGACCATAAAAAGGTTGCAGATTTGGGTAGTGGAAATGGTTGTCCTGGTGTCATCTTAAAGCTGTTGTTTCCCCAAATCAAAACTTTGGATTTAATTGATAGCAAACACAAAAAGGTTAACTTTTTAAAAGAGGTGATTCAAACACTGGAACTAAACAATACCCAAGCACTTTGTGCCAGAATTGAAAACCACACTGAACAATACGACACTTTATGCTCCCGCGGACTTGGCAGCATTATTGAGGTTAATGCCTTTGCTTTAAAACTGTTAAAGCCCAACGGCATTATCTTCCACATTAAACAAAGCTTAGATCAATACCTCGAGTTTGAGGATTCGGAGCAAAAAGACCAGTTTAAACCACTGTTCTTTAAGTTTTTCCATGGCAAGCGCCAGCAAATCCTGATTGCCATGAAGAAAAATGTTTAAGTTAGGTTGAGCTAGCTTTGTCGATCCAGAGTTAGCTAAAACAGCTTTCTTGGAATTTGCCAAACAGTTTTATGTAACTAGTAGTGCTGGTGCAATTCTGTTTGACCTTAAAAAGAGTCAGGGCTTAGACCAACTGCAAGCAATTGAAAAAACAAGGAAGGTTATTATTACAGAACAGTTTGCCAACCAAACTGGTAAATGAATCTTGTTTGACAAGGAAAACACTAAACGGATTAACAGCTTAGCGCAAGAGCACATTACTCCCTTAATAAAACGGATTTTACGCTTAGCGGACTTTAAAAATGTCTTGATTAATGTGCAGCACCACAAGAAGCTGCAAAAATGTCTGTTATGGGAAATTAACGGTTTAATCTGCTTAGTTGAAAGTTTACAGTTCATGGAAAATCCCACTGCAATCATGGAATGATTCCAGGGATTAAAAAAACACTGTCCTAATGTAGCAGTTGTAACCATTAGTGGTCAACACAAACCAGTCATAGAACCGAGCTTAACCGAATATAAGGCAGTATTTGGTAGTAGTTTACTCTCCTTTCACTTAGATGCAACTACGATAAATAACTCCCATTTAGTGCGTCAAATTTTGGAACAGATCAAAATAAAAGCTACCTTAAAGTCAAACTCTAAGGTAGCTAAAAGTTAATGGCGCATCGGGTGGGACTCGAACCCACGACAACACACTTAGAAGGCGTGTACTCTATCCGCTGAGCTACCGACGCATTATTCTCTCCAGAGAGAGCAAACCATTTCCAAGGGGCCACTAGGACCCCGTGAAAGTTCGGAAGCGGGGATCTTATGGATGATTAATCCCAGTTCATTGAGAAGATTATTGGTGTAGTGGTTGCGATCAAAGACCACAATTTCTCCAGGGCTTAAGTTTAATACGCCCATACCACAGTCCAAGAGTTCTTTACGCTCAGCCACTTCATCACCACCACCAATAATAACAAAGCGGGGTTGTTTATCAATAATCGCTTCAATTATCTCACCAAAATTAAACTTTAGCGGTTTGAATTTAATTTGTGCTAAGCCATCCACATCCCTCTGGGCGGTGGCTGTGAGTTCAAAGATTTCGGTAGCTTGTAACACTTTGCGCGCAATAATGAACTTGTCGACATCCACCATCGCTATAAGTTTATTGAGATTGGTCTTAGCACCAAACTTGTGGATATTAATTGCAATAATCTGCTTAATTGGATTAGTGTGATCGCTTAATAAATTACGTGCCAAGCTTTCAATTCCCAACACATCGGTGTCATGACTAATACCAATTAGCAAGATTTCATTGCTAATTTGAATAATGTCACTACTGTTAATCAAACAGTTCTTAAAGTCACGGTTAGTATAAAGCTTAACTCCATCAAAACGGGGGTGAAAGCGCATGATGTATTCATACACTAACGAATGGCGCTTTAACTCAGAATCATTAGTGACAAATAAAGCGACACCCGTTCCTAGGGTATTGAAGAACTTATGTGGATAAAGGGAAAACGATAGCGGTTTAATTAAAAACGGTGAATTACTTACATGAATGTCGGCAAACCGTTGCGGGGTAATCGGTGGTACATCTGATACCTTAATGCCACCAAACATGACCTTAATCACATCTACAAGCGAATCTAAACTGCGAAAGAAGTTTAAGCAAGCTTCGAAGGTACTCACACTTTTAATGCCTGATTCCAATAAAAAGTTCTTAATGAAGTCCTGGCGCACATTAGGGTCAGCCGCACTTAAAGCTTCTAACAGTAAATCTTGTAAATAGTGCACCTTTGTACCATGTTGGCGCAAAATCTTAATAAATAACTGGTGTTCCTTTTGAGCGCGTTCTAAGTACATTGCACTGCCAAAGGTCTGGTTATCAATCACTCGTTCGGTTTCGATACCGGGATGATGGGCAATTACTTCCCTTAAAAAACTAATTTCACTAGTAACGCGTACACCACTGTTAAACTGCTTCTTTTTTAACTGTAGTGCTTTAGTGTTTGGTTGATCTAATTGGTTATGCCCATCGGTTTTGACAAGCTGTTTTTTTGACATGTAAATTCGATTAATTTTAAATAATTAACAATTGCTTTAATTGCGTTACTTCCATGGATAGTAAAAAAGGAATTTTAGTGGCAATTGGTGGCGGGAGTTGTTCTGGTAAAACTACGATCGCTGACATGATTTACCAACTTTTGCGTAAAAAGCTTAAAGTTGCTATTCTGCCCCAGGACAACTATTACAAACCATACAAGAACAAATCAATGGCGCAACGCAAAGCGATTAACTTCGATCACCCCGATGCCTTTGATTGAAAGCTGTTGTGAAGTCACCTCGATAACTTACTAATGGGTAAAACAGTGGCTGTGCCAATGTATGACTATGTTAATTACACCCGTAAAAAGGAAACCATAGAAATAGGTCCTTTAAATGTAGTCATCTTGGAAGGTTTAATGCCGTGGTTTGATGAACGGATTGCTAAATTGTGTAAGCTCAAGATCTTTGTGGAAGCGACTGGAGAGGAACGCTTAATTCGCCGGATTGAACGGGACTGGGAACGCGGTCGTGATGTCGCTTCTATCATTAAACAATGACGCGAAGCAGTTAGTCCAATGTACGAAATCTTTGTCGAAAAGATGAAACAGAAGGCAGATTTAATTATTCCCTGGAGTGAAAGACACGAAGTGAGTACTAATGTGCTCGACTTTGCAATTGAACACCTCTTTCGCAAACACGTGGATCCCAACTAGATTTGGAAGGAATAAAGTGGTTTAAAGGTTTTAGGTAAACTACGCTTGTGCTGGGAGCTTTGGCGCAAGTGGTCAACCCGTTGTTGTTTCGTGGCACTTAACTGTAAGTGGCCCTGAAAGTACTGGTCCAATTCCTTATACGTCAAACCCATTTCCGCTTCGTCAGTCTGCCCGGCAAACAGACTAGCAGTAGGGGCTCGTTCAATGACCAATTCAGGAACATTGAAGTGCTGGGATAAAGCATAAACATCGCTCTTTAACAAGAACGCTAAGGGTGCCACATCACACGCCCCATCACCCCATTTAGTAAAGTAGCCCAAGCTGTACTCAATGAAGTTGCCAGTACCCAAAACTAAGTAGTTGTGCTTTTGGGCATGGGTGTATAGACAAGCCATTCGTAAACGTGCTTTGAGGTTGCCGGCTACCATTAACTCCTTTTGGGGATCAATGGTCAATGCTTGCAACATAGCGCGGTAAGGTGGTTCTAAATCCACCTGTTTATTGTTGAGTTGTAATTGTTCCACTAAGGCAGTTGTGGCCTTGTGATCTAACTCGGAGTTATTAATGTGCATTACCAAGGTTAAGTGGTTTTGCGGAAACAGTTTTTTAGCCATGGCGGCTACAACTGCCGAATCAACTCCACCTGACAATCCAACAACCACGCCCTGAGCGTGAGCTTCATTAACGTAATTGGTGAGTCAAGCTTCCAATTCGTTTAAATAGTTCAATAAATTTTTAGTCATTAATTACAAATTCCTGGCCAGCAAACACAATGACATCGCCTTTTTTGGCACCTGCCACCTTTAATTGGTCTTCTATGCCCACTTCTTTAATCTTATTACCTAAACGGCGAATGTTGTCAAGTGTAGTTTGGGGAATCTTGTCAAACCAATAATGTAAACGCTTGCATTCAACATGCCACCGCCCCAAGGCATCACGTTCAATGTTGAGTGGATCATGGTCGGTTTCACTGGCCTGTTCAAAGACATAGTGCTTCTCCATTTCCATGGGTAAATGAAACTTATTGGCACCGAATTGCGCTAGCGTTTTTTGGTGGAGCGCAAACACTTGGGCCACTAGATCACCCAGTTCTTGTTTGAGTGCACTAATTTTGAACAAGGGAATTCCTTTATTTTCGAGGTATTGTGCTAACTTTTGAAAACGGGTACCATCAAGGTCAACGTCTGTTTTATTGGCCACGACCAGCATCTTCTTTTTAACTAACAGCTGGGAGTACTTCGACAGTTCACGCATAATTTGTTCATACGCTTGACAAGGATCGTCATGATCCACGGGATCGAGTGAAATTAAGTGAATTAGGATTTCACAACGCTCAATGTGGCGCAGGAAATAGTGACCCAGACCACTACCCTCACTGGCGTTTTCAATTAACCCGGGAATATCAGCAAACACCAAACTTTGGTCGTTGTGTTTAACTACCCCCAATACGGGCACTAAGGTTGTAAAACGGTAGTTAGCAATCTTGGGTTTAGCGTTGGACAGTTTGGAAATTAAAGTGGACTTACCGGTGTTGGGAAAACCCACAATCCCCACATTAGCTAAATACTTTATTTCCAAGCTCACATGCAAACTTTGACCCTTGTCGCCGTTTTCATACAGATTGGGAGCGCGCATAATTGGACTTTTAAAAGCTGCGTTGCCCTTACCGCCCTTGCCACCGTAACAGAGAATAAAGCTCTGTTGGTCATGAACAAAGTCCATTAAGATCTGGTTAGTATCAAGGTCACGCACCGTAGTTCCCACTGGTACCTTAATAACCAAATCTTCCCCGTTTTTACCATGGGCTAAATCGGGTTTGCCATTGCCGCCACTTTCAGCAAACAGGTGTTTTTTATTCTTTAAAAAAAAGAGGGAATCGCAGTTGTGGTCGGCTTGCAACACAACGTTACCACCATTGCCGCCATTACCGCCACCAGGACCACCCTTGTCATAATGGGCTTCCCTGCGTCAAGCAATAATGCCATTGCCCCCGTTACCCGCACTAAAGCGGCACTCACAATAATCTGTTAATCCCATGGTTGAGCTCGCTTGGTTTTAATTTTCGTATTGCTTATGTTGGTTATTAAAACCAGGTTAGTAAGTTTATTAATTAAAAAGCAGCTTGACATGAGATTGTTCAAACAAAGAGCAGCTATCCCAGTGTTTCAAGTACGGGGAAACAGGCAACCATAAAGGGACCATGTACCAACATGCTTTTTTTGAAAGCTAACTTAATTTGGTTAAAAAATAACCACGGATTTAATTAAGTCCTTCGGTTTATCTTTCATTAAAAACAGGGCTTCTTCAATGTGTTCAAAACCCTTAAAGCGATGGGTAATCATTTTGGAAGGATCGAGTTTTTTGGTCATAATTAAACGTGCTAATTTTTCTAACCTTAACCGACCACCAAGCATAAGACCACCATGAATGGCCTTGTGAGCCATACCAACGCCCCATTCAACTCTAGGAATAGTTAAAAACTCACCAGCGCCAAAGTAATTAACGTTACCAATTTTGCCTCCTGGTCTTAAAACCTTAATCGCTGTTTCTAAGATAGTCGAACCACCACCAGAAATTAACACCTTTTCCATTCCTTCATTGTTGGTTCACTTCAAGATTTCATCCACAACTGAAACCTTTTTGAAATCAATGTAATCAGTCGCACCGTATGCTTTGGCAATTTCCACAATATCTGGTCTAGAATCCACCACAATAATGCGTCCAGCACCCTTTAAGGCTGCACCAGCGATCGCCATTAAACCAACTGGACCAGCACCAAAAACGACAACGCGACACCGTATTGGATGTCAGCTAGTTCATTGGCGTGAAAGCCTGTGGGAATCATGTCTGACAACATACATGCATCAGCTAAATCCATCCCTTCTGGCATCAATGCTAAGTTACCCTCAGCATCATTGACGTGAAAGTATTCAGCAAACATGCCATCTTTAACGTTGGAAAACTTCCAACCACCCAGCATGCCACCGGAGTGCATAGGGTATCCAGCTTGAGCATTAACCGAATTTCATTCTGGTGTAATGGCTGCCACTAAAACGCGATCACCAACCTTAAAGCTTTTGACCTCAGACCCCACTTCATCCACAACACCACAGCCCTCATGGCCTAAAACCATGTTGTGACGTTCACCAATGCCACCTTCCCAAACGGTGTGGACGTCTGAAGTACATGGTGCTACGGCCAGTGGTCGAATTATCGCATCATTGGGCCCACAAACTGGTCTTGGTTTTTCGATTCAACCAGTAGCGCCAATTTTTAACATTGCATATGCTTTCATAAAACTTTATTTACCTCTGCTGTTGCTTTAATAACTGAATTTTAAATAAACAAAAATTATTAAAAGCAAGCGTCTCAAACTATCAGGTAAGAACGCCTTCAAATGGTTTCAAAGAATGGTAAGCAGTTGTAGTTAAAAGCTGTGTTAATAAAACACTGGAGAAAATGGCAAATAAACTAATCCAGCACTAATACAAAAACTGACAATAAGTTATTAATCACTTGATTTAACGCGCTTTTTCTTGATGGTAACAACAACTGTTTTGCTCTTTTCTTTATCTCATTTCGTTTTAATGTCAGACATAGCCTGTTGGCTTTGTTGTTTGACTTTACCTGTGTGAACCAGTCCTTGTGCTACTACCATGGCATAGCGTGAACGTAGTAAAACTAAGTTAGTTAGTTTATTAATTACAAAGAAACTCAGCATTAACACTACCAACACTAACACAAAACGCCCAAAGATTACCCAAACGTTTCAAGCTTGAGGAAACATACCACTCATAAAGAGGCTAATCATCATCAAAGCAATTCCATAACAAAAGGGAATTGCCTCCACAATTAGGTATGGGGTTGTAAAGAATAAAAGCAGTAAAAGCAACGCATAAACTACTAAGGGCACTAGTAAGAACCACAGTAGTACTAAATCAAAAATGGGTTCTACTTCCCTACCCATTAAAGTACGGTTATTTGGGGCAATGACAAAGTTAAAACCTAAGGCATTAATTTCCCCTAATAACACCCACACAATGATGTAGTAGACAAAGACCAAAAAGAAGCTAAAGCTCCCGGTTAATCAAGCTGGAGTTTTGCGCTTTGCGGTTGATTGAAAAAACTGCTCCAAGTTAGTAAAGTTGTGCTGCTATTTGAAATTTAACAAAGCGATCGTTTTCGTAAAACTGGTAACGTAAAAGCCCTTTAATTACCCAGAGGATAATTGGCAGGCCATAGCTTTGTAGCTCCGCACAATTTTTGTGGTAAAAGCTATCATTGGGCGCTAAGAAACGACAGTTCTGGGTAATCTCATCTTTAGTTAAATGAACTAACTGCTTTTTACTGTTAAAGACAAAACTAGTTTTAAAGCTCTTTAACTTTTCGTTGAGGTACTTAATCGTGGAAAAGTGACTGGACAGCAAGATTAACTTTTCTTTAGCTAACTCAAAACCTTCTAGTAGTTGTGCTAACTGTTCTACTAGTACCTGGTTTTGTTGTTCTTCTTCGCCCTTAATAAAAAGCAAGAGAAAGCGAAACGGGGTTAAAAATTCCATTAACACCTCTTTTAAGGTCAAGATTGTCTGTTCTTGGACATTTACCTTAAACTGCTTCGCTAAGTTAATCTTTTTGAGATCAACTAGGTTAATATCACTCAGTTTATGGGTTTTATTTCCTACCTTATAGTTGTCTAAATTAGTGACTACTATTTGTTTATCTTTAGTGAGTTGCACTTCCATCCACAAACCATCAAAACCAAACAATTGTGCAGCAGCAAAGGCTAAATGAGTGTTTTCAGGAGCAATGGTGCTGTAACCACGGTGTGCAATTAAAAACTGTTTGCGCATAAATTAGTCTTCGCCAAATACCTCCTTAGCATCGGAATCGGAAAACTGATCTAATCATTCATCAGCATCAATCGAGTCCACATCAAAGCTTTCAAACACAGGGATATCCTTGGCTATGTCAATGTTCTTCGCATCTTCAGCGTTGGAACTGTGGAATACTAATCAGTTACCCTCACTGTCAAATTCCCCATCGTACCACTTTCATTCATTATCTTCATAGTAACCATAGTCTTCGTTGCCTATTAACTGCTCCCAGAACCGTGGTGCTTCACTAATCCTTGGTTGATGATTCTGATAGTGTTCATCAACTAAGACTCATTCACCGGTCTCATCATAATAACCGTATCAGATCCATTCTCCTTTACGGTTATATCTACCATAGATTTCATCACCTATTAGATCTGTGTAATCACTTTCACGTGCCCATTCAACACTAGCATCTGGTGTTCATATTTGGTTGTCATCAAAGTAACCAGCTCATACCCATTCCCCACTAGGTTCATAATGACCATAATTGCTATTACCAATCAATAGTTCTCAATCAAGTTGCTCTTCTTCAACGTCATCAACTACTTTTGTTGCTTCAGTTTCTGGTTGAAGTTCTGGAGCTTCAGATGTTGCCTCTACTTCCAAGGTTTCTGGCGTTACCTCCGCTTGAGCTTCTGGTTTAGTTTCAAAATCTGAATTCAAACTTACTTTTGGCTCTGATTGAACTTCGGCTTCGAATTTAAATTCAATTTCTGATTCAGGTTGAACTTCTACTTCTGTTTCAAAATTAGGTTCTGGTTCAGACTCGAAACTTGATTCTTGTGCTAGCTCTTGTTGAACTTCAGTTTCGGATTCGAAATTAGGTTCAATTTCTGGTTCAAGCTCTACTTCTGGTTCAAAATTAGGTTCTGGTTCAGTAGTTTCAATTGATTCTGGTTCAGGAATCGTTTCCAAATCAAAGTTAGGTTCTGGTTCAGGTTGAATTTCTACTTCTGTTTCAAAGCTAGGATCAGATTCAGTATTTAACTCTGAAGTTTCAGCGTCTGCTGTTAAAACAACTTCTTCAGCATTTGCTTCTGTTTGCGCTGTAGCTGTGGATACCCATTTGTTATCTTCATCGAAGTAACCGGTTCAAATCCAGTTTTGCTCTGCATCAAAGTAACCGTATTCACTGTTACCAATTAACGCCTCCCAATCCAGTTCAGCTTGGGCTTCAGAAGTTGCTTTTGACTCGGTTTCAATAGCTGGTTCGACTGCAACAGCTGTTGACTCTTGTTCAACTTGCGCTTCTTCAACGGTCTCTGCAGCTAATGTTGTTGAAGTTTCAACAATTGGTTCTACAGCAAGTGGTGCTTCTTCAACAACTGCTAATACTTCTGGTTCTACTGCTTTCTCAGTAACTGTAACAGTTTCAGTAACAACTGGTGCTGTTTCTAGTTGAACAGTTTCAAGCGGTTCTAAACTTACTGGTGTTTCTGTAAGTTCAACCGTTTCAACAACTGGAGTTGATGCAACATTAGGTTCATTTGATGTTGCACTTACAACAGTTGATTCAACAAACTCGTTTTCTAAAAGTTGTTCTTCAGGTTCTACTGTTGGAATTACAACTGGTTCAAAAACTGGAGTTGATGCAAAAGTAGTTGTTTCTCTACTAGTAGTTGTGACAAACTCATTGACTAGCTCTTGCTCTTGCAACTCTATTTCTGCTACAGTTAGTGGTGCAGTGCTAGTGAGAAAATCAACTACATAATGAAACCCAGCTTGTTGGGGTTGACCGTTGTCAATCACCACCACCTCTTGAATCAGCTCGCTTTGTGGTAAGGCTGTGGTGGCTGTGGTGCCAACCGCCACTAATTCATCAATGAGGTCAGCTTCATTTAAAGCAATGTTAATTGCTTGAGAAGGGGCTTGACTAGCTGCAGCTGCTGTTTGGCTTGGTTTTTTGAAATCAAAACTTTCATTAACAAACTCAACCTTAATAATTGGTTCTTGAAACTGAATCGAACTTGATGCTGGTTGTGCTTGTACAGTAACCACTTGAGGTTGAGTGGTAGTTGTTGACGGTGCAACAAACGGCACTTCTACCATTTCCGTTTCCACTTCCGATTGGTCTAACAGTGGTGCGCTAACACTTAGCTGTTCTAGCTGAGTAGTTTGATTAAATACAGTGGTCTGCTCTTCTATGACTGGCTGTGATGTAGAAACGGTGACAGCAGCAGTTGGTGGTGATGTTTGGTCTACTACAGTTACAGTTGGTGGTGCTGGTAATTCAATTTGTACAACAGGTGTAGTTGTAGTGGCTTTTTCAACCATTGGCAAGGTAAAGCGCACTGGGTCAGGAATCCAATTGAGTTTGGTGTCAAAGAAACCCTTCCACACCCAAATGTTGTTTTGAATGTAACCATAGTTAGGATTACCAACCAGTGGTTTTCAAAAACAGCTATCAATCTGTTTGCGCTTAATAAAGGCAGTATGTTCTTGGGGCAGTTCCACTGTTTCTACCACTTCAAAGTAATACCAGTTTTGGTGTTGATCAAAGAAACCTAATCAAATTCAGGTGTGGTTCTTTCAAAAACCAAAACGAACATCACCAACAAAGATTTGTCAAAAGAGTTGTGCTTTTTCAAAAATCGATTCACGTTCTTGACTGTGGCGGGTGCTGGTTAGAACCTGATTCTGTTCTAAAGCAACCGGTTTTAGTTCAAATTGTTCGGTTTCAACAAGCTCACTGTTGGCATAATGTGCCAGTTGATTTTGACTAATAGCAGTTTTGCTAACAGTCTCCTCTACCGCTTTATTATCACCCCGGTCATCGTTAATAACACTAGTACCAGCTGGTGGCACAATAAAAACACCACGCTTTTTCTCGTCAAAAAAGTCAATAGAAGATTCAACTATCTGTTTTTTGACTTTAGAAGTACCTCTGGTAGCGGTGCTAGCTCTGGTAGTTTTATTCTTCTTAAGTACATTGGTTTTGATCTCCTCTAAGAAGGGCTTCTTGAGTAAATCATCGATGCTAACATCCTTATTTTTAGCTTTAGCAGTTTTCTCTTTAGACTGTTCTAAATACTTTTTGTACTGTAATGTAGTGTTCGATGGGTTCTGTTTTTTTATCGTTTTTGGCATTAACAAACATGCTTAGCACTAAATGCCTTGAGGCGTTTTGCGATTAATAAAATTTTAATTTTTAATATTAATATAACGAACTTTATAGCGCTAGCGACACTAAATTAAAGAGCCATAAGGTGACTTTTTTTGTTTAGGGTCATAAGTCCTTACAAACAATTCCACAAAGACATTGCAGTCATAGTACTCCAGTAGTTTTTGACGCATTTGCATCCCGATCTTTTTCACCATTTGGGCATTACGGCCAATAATAATTTTTTTTTGACTAAGTTTAGGCACACTAATGGAAAGGTGTAGCTTCAACAGGTTTTTAGTTTGATCAAAACTCTTATCGACAATCTCTAGCCTTACTACATGGGGAATTTCTTCGCTGCAGTACTTAATGATCTGTTCGCGCAGCGCTTCTAAAATCTTAAAGTTATCAGTGTCAGCATCTAAAAATTCAACATCTTTACGAAACTCATTTGCTTGTAGTTTCACTTCGTTAAACTGGTTTCAAAAAGAAGTGGCATCAAAATGAAGCAGATCAGTTTCCAGTACTGCTTGCGGTTTAAAATCGAGCACAATTGCTTGCTTGTCCTCCGCTTGCAAAACAACATCAAAGAAGTTATTAATTAACAAAACACGAGTTAAATCACGGTAACGCTTTAACGGTTGTAATTGCGTTTGTAACAAGGGTAACTTTTGCTGTTGATCACTGCGCACCACTAACAACAAGACATCTATCCCACTTAAAGCCTTGCGAATCTCTTGACTAATTAACTCATAGGAGCTGTGACGTTTTTCACTAAAGCCAGGAACATCAATGAAGGTAATGTTTTGCTTTTCATGGGTAATAGTATTAGTGCTAACCGCCAACAACGTAGTGTTGTTCATAGGTGAAACCAACAAGTTATTCGCTTTGTGTAATTGGTTAACGAGAGTTGATTTACCAGCGTTGGTTAGACCTAAAACACCAATTCTTATCGATTCCATAGTTTTAAAACGGTTTCATGAATTTCATCTTGAAAAAACATCGTTACACTTTCAAAGGCCAGACTTTGCTCATGGTCAAACTCAAACAGGTGTAGCAAACCATGGATTAAGTTGCGTGTTAGTAAAAACCAAGGTGTGCAACCAAACTGCTTGGCCTGCTTAAAGATGTGTTTGGGACACAAAAAGATTTCACCAATGGCTGGTGAAAACCCCGCCTCATTTTCGTTGTAACAGATGCTAATCACATCAGTTACCCCTTTGCGGTTGAGAAACTGCCGGTTTAAGCGCTTCATCTTTCATGGTGAAACAATCATAAGCGCAAATTCTGGTTGAAAAGAAAGCTGCAAGCTGTCTTTAATAATTTGCAAACAAAGCTCGATGCCAGCTTCAAAGTTTTTGCCAAAAAAGCGCTTAAACAAGTAGTTAGAGTTAATACTAAAAGACGGTTTCAAACAGCGCTTTGGACGGTAAAAGAATCGTAATATTATACCAACCTTGTTTTAAGTTAATACTTAAATTAACCGCTTGAAAAAGCTGTTGCTGGTTTTCATTTTCTAGCTGCTCTAGGGAAATTTTCTTTTTGTAAAGTCGAAAAGCTTTCTTATCTTCCTTTTTAGTTGTTTCTGCTTGAAAATTAGTTCAAGATACCTGGTTAAACTGGGCTTCATCCTGCTCGTTTTTAAAAACTAAAAAGGTTTGTTCAGACAATTTTGCCCATAAGCTTGTAGTTAGGTATCGTTGTACCTTAACCGTAAATAAGATGGCAACGGCTGTTAAGAATAAAACAAAGAAAGCTAGAGTTAGGCCAATAATTTTTTGCTTCATATTATGTCTGCTGTAAATAAGGACTGAGGTTAATTAGGTCTTGCTCACTGTTTACAAAGCGTAAATCGTGTTCGACCATCAGAACAAAGTTGTTATAGGTCAACGGTTTGATCAAAAGCTGATACACGCGCGGTTTGAGCTCCGTGGCAATGGCGTTGAGTGTTTCATCTAACAAAATCACCTTGTCTTTGAGGTTTTGTAATAAGAACAGTTGCAAGATCTGTTTTTGTCCTGAACTTAACTTAATCTGGTTGTGGATGCCGGTGTCGGCTAACAGCTGGTATACTTGGGGATTTTTACAGTTTAACAAGGCTTCACTGTAAAGTCAGTAATGTCGTTGTAGGCCGTTAACTGCCCCGTTAAATAGTAAACGTTGTGAAACAGTTGGCTTGCACTAAAATCCTTTAGGTCTAATTCATCCAACTTAATTTGACCCTGGTATGACAAACCTCGACCAGTCAGAATTTTCAGCAAGGTACTTTTACCGCTACCATTCTCACCACTAATAACCATGCCATTAGTTAAATTCAGGTTGAGGTTAGCCAAATCGAGATTTTGGTTCAGAGTGATACTACAGTTGTTTAAGTTAATCACTTTGGGACACTTCCAACTGACCTTGAAATTGCAATCATTAACCTTGTCTTCAAACAAGGTAACTAGTTTGTCCTTACAGAACTGGTACAGGTTAACTGCTGCACCAAACTGGATGATTCTCGTGGCATAAGCGGAAAACAATGCCTGAATGGAAAAGATATAAAAGAGAAAGGCTAAGGTGTAGCGCTGTTCAATAATGCCAATAATGCCCAAGGCCAAAATTAAAAAGTCAAATACCTGTTGGGCAAAACTTTTAACACCATCACTCGCTAAGTGGTTCACTTCCTTTTGATTACTAATGTTTTGGTTTTTAATAAGTGCTTTGCGCAACTGCATCATTAAGTAATTGAAGCGCTTGCCGTTTTGTTCATCACGCAAATTGCCATCTAACTGCAAACTGACCTTGTTTTGTAACTCCATTTGCGGAATTGCTTGCTTCGTAATCCGTTTAGTGAAAAAGAAGTCATAAAGAAAAATGGCACAATTCACCACAATTTGGGCAATTGCTATCCATAAGAACTCCATTTGGTTAATACCAATTAAGGTGCCAATAATTAAAGCTACTGTACAGCTAATAATTAAGTTAGGAATATGGACATTAAAGAAGGACAACACTTCCTTGAGGTAAAACTGACGCTCGGTTAATTGGTTAGGTGATTTATTGCTAAAGTAGACAAAACTCTTGGTTTGTAATGTTTGCATAATTCAACCCAAATAGCCCCGATACTGCTTTCAAAACAAAACATTACGCACCATTTGCAACAAGGCATACAAAAGTAAGTACAAGCCTTTTAAAACTAAAAAGAAAACCACAAAAACGACAATGTTAACTGATGTCCCAAAGTCAACGGTGTTGGCAATCATCGTTTTAGAGGCCGTTGCTAAAAGTGTTGAAATGCCAATAATGATTAGTTCAATGAAGACATAGAAAATAATCAGGTTTAAATCAAAGAAGTGCTTAAAGAAATTGCGCTTATTAATGGGCGGAATTTCTTTAAATGATTTTTGCACTGTAGCGGCATAACCAGTTCACTGTTTCTTTAATTCTTCATCATTTAAAAGATATTTCCCTTTAGCGGGGTCAAATACTTCCCAGAGATTATCTTGTTTTTTATGCACCACCACAAAATGCTTAGGGAAGTGGACAATAAAGTATTTACCCTCTAGTTGTTTGAGCTCTTCTAGGGTTAGCTGGTAACTAGCAATTTCGAGACCAAACTTCTTGCCATAGGTCTCTAGTTCAAAGAAACTCAAACCACTAGCTGGTAGGTTAATTTGTTCCAACAGTTCATCATGGGCATACTTATCCTCATGCAGTTCATTGGCTAACATGCCAAGCACACAAATGCCACATTCGTTGGGTTGTTCTTGATAAATAATTTTCATGGGGCTTTATCACTTACATAAATGACCCAATGAGGCTAAGGTGCCAATAAAAAAAGGGCTGTACTACTACAACCCTTCTTTTATCTGCTTATTGAAAGCCTGTTTATTCCTTTGCTTGCTGCGCAGCACGTTCGTATAAAGTACGTACTAAAACCCCTTGACCAAGATCTTGAATCGAAGCTGTGGATGCAATATCACAGTGGATCAAAGGTACCCCTTCCCTAAATTCAGCTAAGAAACATGCCGCCCGGGATGAACCTGCTCCCTTAACACTAGTGGAGTTTTGTAAGTCCGCTAGTTTTGATTCCAATGGTTTTAGGTAATCCGGGTGCATTGGCAAGCGTCATACCGGTTCGCCTGCACAGCAAGCTGCTTTTTTAAAGGCATCCCATGCCATGTCACAGGTACTAAAGATCCCGGTGTAAGTGGTACTTAAGATGTAAGACATTAAACCGGTTAAGGTAGCGACATCAATAATTTGGGTAGCTTTAAGATCCTTAGCAGCATAGCTAATGGCATCGGCTAACACCAAGCGACCTTCAGCATCGGTATTGTCAATTTCAACCGTTTTACCATTGTAAGCGGTTTGGATGTCATCAGGGCGTTGTGCGTGGGGACCTGGTAGGTTTTCTGTTAAAGCAGCTACTGCTACTACATTGGTTTTCACCTTGTTTTTCGCTAAGGCCAACACGGTGGAACACACAATAGCTGCCCCACTCATGTCGTACTTCATACCACGCATGTAGTCACCGGTCTTAATGTTCATCCCACCCGAATCATAGGTAATGCCCTTACCAACTAAAGCTAAAGTTTCACTGCTCTTCTTATTATTGTTATAGGAAATTACTAGTAAACGCGCTTCACGTTCCGAACCCTTGTTAACCCCTAACAGTAAGCCCATCTTCTTTTTGATGAGATCAGCTTGCTTTAAAACGGTAATCTTAACACCTAAACCAGTAGCCGCCTTTTCAAAGCGCTTCACAAATTCCTCTGGGTAGAGTTGGTCAGAGGGGGTGTCTTGGAGTTGACGCGCCATGTTGACATAGTTACCCACTACCTCGTATTCTTTAATGAGGCTCGTGTGTTCGCTGTCACACTTCACTGCTACTTCCACATTCTTTTGTGGTTCGGTCTTCATGGTGAAAGGTGTTGTTTCACAGAAGGTAATGCTAGCTACCACTACCTTTAATGCTGTTTTGTAACAGAGGTTTTCTGCTTTGTAGAGTTCCACAAAACTGTCTAAGTCCACGTTTAGTTGGTTCTTTTGCGTTTTCAAAAAGGTAACTAGAGCGCGGTTGAAAGCGGGGAAATCAGTCGGAATTTTTACGCCAAATTCAATTTGGCCTTCAACACACTTAGTCTTACAGCAATCACTCTTGCCGTACTTATCGGACTTTTTAAAAACAACCGTCTTGCTGTCAAATAACTTATTTAATTTCATCTTTAATTTTTACCTATTCTTGGAAGCTACCTTCCTTGTTTTCCTTAATATCATACACAGCAACGCTGGTGGTAATTAAGGAACTTGCTACTGATGCAGCTTTTTCGAGTGCAGTTTTGGTTACCTTAGTAGGGTCAATAATACCGCTGCGGATCATGTCGACCTTCTTTTTGGTTTCAGCATCAAAACCAACACCATCAGCTTCACTGCGTAGTTCTGCAAATACTTTAACTGGGTTAACCCCGGAGTTTTCAATAATTTGGCGCACTGGTGCTTCTAGTGACTTACGCACAATTTCATAACCAACCAGGATTTCCTTGAGGTTTTCCGCACTAGTTTCTGCCTTGTACTTGTCAGCCAACTTACTGTCATTGAGGATAGTGGATACGTTAAGTAAAGCAATACCACCCCCGGAAATAATCCCTTCTTCAACAGCTGCCTTAGTCGAGTTTAAGGCATCTTCAATGCGGAGTTTTAACTCTTTTTGTGCTAGTTCCGTAGCACCACCAACCCGTACTACAGCTACCCCTTGACTGAGGTGGGCAATCCGTTCCTTTAACAGATCGGTATCGTACTTTTCGGTAGTTTGCTTTAAACGACCATTTAGAAGGTCAAGGTGTTTTTGGATCGTTTCCTTAGAACCCTTACCACCAATAACCGTGGTTTTTTCTTTGGCTACTTGCACACGGCGAGCTTCCCCTAAGTGGTTTACAGTCACATCCTTAAAGCCACCACCTAGTTCATTGTTGTAAGCTAAGGTGCCAGTACTAATGGCTAAATCTTCCAAAGCTGCCTTTTGGCGTTCCCCGTATTCGTTACATTTAACAGCAACTACATTAATGGTACCGCGAAGCTTGTTCACCGCTAAAGTAGTTACCACTTCTTCAGCAAAGTCAGGTGCCACGATTAACAGTGGGTTACCGTTTTCCATGCTACCTTCTAGTAACGGTAAGATTTCCTTAATGGTGTTAATCTTCATCGCACTGACCAAGATCTTCGGTTGGTCTAACACCACTTCCATTTTTTCTTGATCGCTAACCATGTAAGGGGAAGCGTAAGTACCTTTAAACTCAATCCCTTCAGTGGTTTCTAGGGTAGTGTTAATCGTCTTAGCATCATCGGTGGTAATGACACCGTTTTTACCAACTAAGGCCATAGCTTGAGCAATCAGCTTCCCAATTTCCTTGGAACCGGAAGAGATCGCTGCAACTTGCTCGATTTCTTCGTTGGTATTAATCTTTTTGGAACGCTTATCTAACTCAGTAATAATTAACTGACTAGCATCTTCAATCCCACGGCGGACATTAACTGGATTAGCGCCATTATTAACAGCTTCCACACCACGGTTAGTCATTTCCTGTGCCAAGATGGTAGCAGTGGTAGTACCGTCCCCAGCAATATCATTGGTAGATACAGCAGCTACCGAAATAACCTTAGCACCAATGTTTTCTAAAGGATCAGTTAGTTCAATTTCCTTAGCGATGGTCACTCCATCGTTAGTAATTAAAGGATTGGAAAACTTCCGACCTAAAATCACGTTTTGACCTTTAGGACCAACGGTTACCTTAACAGCGTCTGCTAATTTATTAATACCAGCTAGCAGTTTATTGCGGGCATTCTTGCCAAATACTAATTCCTTTGCCATAACTATTTTTTCTTAGCTCCTTTCTTTACAGTAGTAGTTTTTGTATTACCATGCTTTTCAAAAGCAAGGACATCTTCCAAGGAAATAATCTTGTACTTGGTACCTTCCTCTTCAAAGGAAATACCACTGTATTCCTTAAAGTAGATCGTGTCACCGATGCCAAAAGCATATTTCGGTTTTTCGCTTTTACCATATGCCGGTCCTGCACCTAAAGCAACGACAGTTCCCTTGTGGGCACTTTCATTTTTATCAGGGTTAGCTAGTGCTGTAATAATCCCTTTTTGTGACACTTCTTCTTTGTTTGATTCCACTAGTGAAACCAAAATGTTGTCATGAATTGGTGTTATCTTCATACCGTCAATATTCTACCAATTATTAACAATTAGCAGTTAAATGCTTAAAGTGCAAATAAAAATAATTTAGCAACACTACCGAAATAGTGCTGCTAAATTAGCGTGCCTGATTTATTTACTAGTAGATTAAGCGTTTATTGCCAATAAGGCACTCAACCTTGTATATGTGGTTAGTAATTAAAACTAATTAAGAGTTATTTTCTTTTGGAAGTGGCAGACTAGCATCAAGCACACCGAAAGCAATTAACATGACTACGGCCACTAACAACAATAAGCTACCGACAACAAAGAAAGCAACAAATAGATTTTTCGTTTTTGCATGAGCGGGACTACCCACTTCAGCGTGTTTGTGCTTATGTAGGAAGATGCCACCCAACAAACAACTCAAGAAACCAATGATTAACAACAAAATAACTAAAAGGCTAATTCCACCAGCAATTTGGAAAATGTTACTCGTTATTCCTTCGGCTTCATTGAAACCAGGAGTTTTACCACTAGAATTCTGGGAACCAAGCATTTGCTTTTGGAAGAAACGCGTAATAAAAGGAAAGCTTAAAGCTAAATTTTGCAGCATGAAAAATATTTGTTTGGAAATAACAAATACTTAAAACAACTAAAAAACCAATGCTGACAGTAGTAACGTTAACGCTAGTTAATCTATCGTATCTTGGTTAGGAAATTTAATTGAAAAACTACCACAAACTTTTCAACACCCATTAAAGGCTTAAAAAGTTTCAACAATAAAAACTAAAAACTCTACGCCATCACTGGCATAACAGGAAAATTTAATTTTTAAGCCTAATGCTAATTTCTTTTAAACCCTAAAAAATTAATTGGACTCGCTAATGAAAACGGTTTTAACTCGATAATCTAATGGTTTTATTACCTCTAAAGTGACCAAGCTTCTAATGGTTTTGAACTGTTTATTTCAATAAAAAATCACAGCATCTTTTAATGAGTGAAGTGATTTTTTTTAAATGCTGTATGTTTAGTAAATTAAGTAGTTATCTAATACTCCAATCCCTTATACAACGGAAAACGTTCTAACAGTTTTAAAACCGCTTTTTTTACTTCTTTAATAACTTTCTTATTACCGTCAGATTTAATAATCTTGTCAATTAAGGCAGCAACATAAAGGAAGTGCTTTTCCTTAAAGCCACGAGTAGTCATGGCAGGTGTACCAATACGAATACCGGAAGGATTTATGGCAGGATTCTTATCGAAAGGAACCGTGTTCATGTTCAACACAATGTTGGCTTGTTGCAATCATTGTGAAACATCTTTACCCTTACCGACATCAAGCGAGAACAAGTGGGTATCAGTTCCGTTAGAAATGACGCGGTAACCTTGTTGTTTGAACCAACTAGCCATGCTCGCTGCATTGGTCTTAACGTTTTGGATGTACTGCTTGTATTTTGGTTGTAAAGCTTCTTTAAAACAAACATACTTAGCCGCAATTACGTGCTGTAAGGGACCACCTTGACAACCCGGAAAGACACCGCTGTCCAATTTGCGAATAATCGCTTCGTTATTGGACATAATTAACCCTCCCCGTGGACCGCGCAGTGTTTTGTGGGTAGTAGATGTAACCACATCAGCGTAAGGTAAGGGATTGGGGTGCAAGCCAGCTGCTACCAATCCCGCAATGTGGGCAATGTCCGCTAAAAGGTAAGCACCAACTTCTTTCGCAATTGCACTAAAGCGCTCAAAGTCAACGGTTCTCGAGTAATTGGAAAAACCGCAGATAATTAGTTTAGGTTTTACCTCCCGAGCAACACGTAGCACCTCATCATAGTCCAACATCTCGGTTTCGGGGTTGATGGTGTAGGAATAAGGCTGATACTGTTTACCCGAAAAGTTAAATTTATTACCGTGAGTTAAGTGCCCACCACAGTTAAGGTCAAGCCCTAAAATGGCATCGCCAGGTTTAAGTAGAGCTAAGTACACGGCATAGTTAGCAGAAGAACCGGAGTGCGGTTGCACATTCGCTCACTTCGCACCAAATAATTCCTTACAGGTATTAATGGCTAAGCTCTCTGATTCATCAACTACCTCACAACCTTCATAAAAGCGTCTAGTAGGATAACCCTCAGCGTACTTATTGGTCAAAATGGAACCAGTGACCTCTAGGATGTCCCTACTCACATAGTTCTCTGATGCTATTAAACAAATGCAATCTCTTTGTCTTTGCAATTCCTTATTGAGGATTCTTCTAATCTTTGGTTCCATGGTCTTACTAATTTACTTTAATTTTGATCAACATATTGAAACTGTCGATTGTTACTCTTTTTAATTTGATCACAAAGATGAGTGTAGTGTTGTTTGGCAAATTGGGTATAGTAATGGGTAGTATTGCGGTTGCCAAAGATAAGATCATAACCAATGCTGCTCTAGTTCGGATTGAGGGAATTGCCCTCACCCTGAAAGCGCAGCATTTGGACTAAACCAACATCGTTACGAACAGCCTTCTTGGGATTAGTAATTACCGTAGCAAAATAGATTCCCACAATTTGACGCTTTTGGTTAATCACTAAAGAACTAGAAGTCCCTTTTCTTAGGTTAACCTGATCGATTCCTAAACCATGACCATACATCTGGTAGTTTTGCCCTAACCACTTATTCTTAATCTTGAATGACTTAGTGTGATCAAACGGTCAAGATAACTTACTACCAGAAAAGTGTTTTGTTTTAATGGTGGTAAGTTGATTGGTAGTCAAAGTAGGAATTTCGCGGTGGTGATCTACTGACCAAGGCTCTTTAAAAGTAACTTGAGCAACATCCTCTGGGCGAGTAGGTTTAACTTCAGTTTGGTTCAAGTTAAGTGCAGCAATATTGCTCTCTACTTGGGGATAACCTAAAGCAAAATAGCTATCTCGCTTAAAGCTTGCCATAGGTTGGTAAGCAAAGATTCCCACACTGTTACCTAACCGTTCATAGGTATTGATGGCGGGGCGCATAAAGTGGTCATAGATCTGTTTATCCATTTGGTTGTGTAAAAAGAGTGGAACTTCTATGATCGCAAAATCCAAATAGCTTTTTGCTAGTGCAGGATCATCTTCTGATGCACGAACTGGTGCTACCCACTGGTTACCGCGGTTTTGTAAGGGCACAAAGTCGCGTGCTACAAAGGCTGTTTTGGGAATAGTGGAAGTTTGTATTGACACAAAGGCGTTGCTTACATCAGTACGTAGCTTAAACAATTGAGGGTTAATATATTTCCCTAGACAAAATACCGTAGTTAAACTGTTGCCAATGCTAGCTTTATTAAACGGTGCATAGTCCTTCGGATTGCTCAAAGCAACGGCAATATGCAGGTTAGTCGCTAAATAAAGATAAAACGGTTCGCTCAGGTTATGTGTTCGCTTCGGTTTCTTTCAATCAATTAATCAACCAGTACCAAACACACGTCACTTGTTGTTGGCAAGCTGCATTGAAAAGCTCAAATCATACAGGTCGTGTAAAGCTTCTGACTTTTTGGGAATAGTATTAATGAGGTAATTAGTCTTTCACTCTTTACGTTTAACACCCCCCGCAGCTGTAACGGTTTTCAAAAGGAACTACAGCTATTTAGGGGAATTGCTACTAATAAAAGGGCAGGTAGCAGAGTTGACCTACGAACACGAAACGCAAATTAAACACTTAAGTTAGCTTAAGTCTAATATTTAAGTGAAAACAAGCCTTTTTGAATAAACACAGATAGTTGCAGAGGCAAAACTGACTTAAACATTCGCTTAAGTCAATTCAAGAAATAAGCAGTAAATGAGCGAATTATTTCTTTACTTCTTTTTTGAGTTTAGCCACAACCTTATGTTGTTTGCACGATAGTTTAAAGCAAACAAAAAACCACTAAGAAAAAACGAAAAAGAGGATGCTCAATTCCAATAACCACCAAACGGAGTGAAAGTGACCCAGACTAATACAAATAATGGAAATAGGCAACTCTGCCATGAAGAACACAATGAGCAAAACGTTGTATATGTCTTACAACTTCATCGCTCGGACTAGTGGTAAAACTTACCATGCTTCGGTTTTGGTAGTTGATTGTTCAGTTAACGAAGGTTGGTTGTGGATGAGGATTTGCCACAACTGCTTGGGGTGTTTGTGTACTGGGTATTGGGCTGCACAACGAACATTTGCAACTACTACCGGTCTGAGGTTGGTTTGGCTTTAGTTTCTGGTTTCACCACTATTGGTTATCTTTGTGGATTGCTGATTGAGTATCAGTTGGACTTGTGATCAAAAGGCCTTCAAGCCGCAAAAACTCTTCTTCTTGGTTAATGTTTTTGCGCCATGTTTCCAAGAATCCCAAAAAGACTAAGACAAAGATAAAGGCAATAACAACTAGGGCAATAACAAGGCTAATTGGCAGATAATATAGAAAAAACAGTGAAATAAAACCGAGTCAGATAATTAACGACACAACAAACAATCACAAATAACACTTGTGTAATTTTCGCGCTTTATTGAGATCACCAGTTTGAAGTTGATCAGTATTATTTTGTTCTTTGTTCTCCATTCAAAATTTTCCCTACATTATTTGTTATCTACTATCCTGTTTGAATTTCCAGATTTACGAACGCGTAATTTTAGCTAAGAGCTTTAAAAATAAGTCAAAAATGGAACTGTTAAACAGTTAAATTACAACAAAAAATAACCGTTACGAAACCATTGAACAGGACACATTTTCGCGATCAATAAACTTAATTAACTGGTCATTACTTTGTTGAATTTGCTCATACAAATGGGTGTGGTGTTGCTTGGCAAATTGGGTATAGTAATGGGTAGTATTGCGGTTGCCAAAGATTAAATCATAGGGCACACAGTTGTCCTTTACCGTCGCTGTAGTACTTTCATCAACTGGCAAGCGTAACAGTTGTACCAGAGTTTTTTCACCAGGTTTAGGGTCGGAATGACTAGCTACTGCAAAAAAGATGCCTGATATTTGTTGATCCTGATTGAAGACTAAACTACCAGAAGCGCCCCTGCGGATATACAAATCATTTAAGGCTAAGCCTTTACCATACTGTTCATAGGTTTGCTGATTAAACTTTTCGTCTAGTTGGTAATACTGGGCGGTCATACACGTGTGCTAGTGTATTGCCAGATGTTTGCCCCGTTTTATTCATTACCAAGGTTGGAATCTGACGCTATTTGTTCAAAGTTCAGAAGCTTTGGTTTTTAACTTTAATGCGGTCTTTAACCGGAACACTAGTTAAAGTGGCACCTGTTTGGTTAAAGTGAATCGCATCAATGTTACTCTCTAGTAAGGGATAACCGAGAGCAAAATAGGTGTCATTTTGTAAATCAGAAGATGACGTACTTGCAAACAAGTTTGTACTATCACCTAAGGCCTTATAGGTAGCAATGGCGGGTTTGATAAAGTTTTCCCATACTTGGTAATCCAACTTGTTGTTTAAGTACAAGGGTACTTCTAACACGGCAAAATCAGCATAAGGTTTTAAACCAACTTCCGGACAAATTTCATTTAAGGATTTCCAATGTTTATTGTCAAACCGATAATCAACAAAGTCCACCGCTGCATAAGCCATCTTTGGTAAAGCTGCTAGTTGGACATCAACAAATGCGTGTTTGACATTCGTGTACAGACCAAATAAACTGGGATCAGTGTATTTACCAATTCGAAAACTAGTGGTTAAATCTTGGGTATTATCGACTTGGTTATAAGGCGGGTAGTCGTGCGGGTTCTTTAAAGTGTCAGCGACATGGAAGTTAGTCGCTAAATAGGCAGTGAACTTATTTTCGTTTGTACTAGTATCCTTTCAATCAATTAACCAACCAGTCCCGTAAGTATTTCACCGTTGTGACGTTAAAGTTAACGAAAAGCTTAAGTTATAAAGCTGGTGGTAAAAGTCAGTTGGTTTGGGAACGGTATTAGTAGCCCACTTTGTTTGGTATTCAGCGCGCGGTACCTTTTCTAATTGCACAATCGTGGCACATCCACTAAGCGGTACGGCAAATAAACAAGACTAGCACTAACAGAAAAACGCCACATACACAAACCTAGACAATTTAATTTAAGTATTTTTTACCTTTGATTCGTTATCTACATATTTTAAACTAGGGTCTCGCGCAGATTTAATTTGCCCATAAAAGTGAGTGTTATTTTTTTGCAAACTGGGCATAGTATTTTTTGGTATTGCTATTACCAAAGATTAAATCGTAAGCAACACTGTCTTTTTCTTCATTAAGAACACTTCGCGGTTTTCAGCGTAACAGCTGCGCTAGACCCCATTCTTGTGTTTCAGTGATTCTACTAGCAAAGTAAATGGCCACAATCTGTCGCTTGTTATTTAAGACTAAGGAACCCGAAGCGCCACGACTTAAACTACCATTAGTGATCCCAATTCCCTTGCCATACATTTGGTATTCTTTGCCACGAAACTGGCGATTAAACGGAAAGTTCTTGGTGTTATCATGGTTTCAAGCCCAGGTGCCACCATTAAACTTGTCACTTTTAATCCTACCCAAATGCGGTCCTTCTTCGTTAATTGTGGAAACAAAGGGCTGATCATTGGGTATTTGGGCTGTTTCACCAGGCACTGTTTTTTCTTTCCCGGTTTGGTTTACAAATAAAGTTGGCAGCTTTTTCGCTATGTAAGGATAGCCCAGTACATAATAATGACTATGTTTAAACTGGTCTAAAGTTGGGTAGGCAAAAATGTTTAAGCTATCACCTAACTGTTTGTAAGCTCTAATTGCTGGTTGGATGAAATGATCAAAAATTTGTCGGTCAGATGAATTATTGAGAAATAAAGGTAGTTCTAATACCGCAAAGTCAGCATAAGGATGAACTTCATCTTTATTTGGCTTAATTTGTACCTTTCATTGCTGTTCTCTTTGTTTTTTATTCTTTTGATCTTTGGATAAAGTGTAGTTCACAAAGTCATGTGCAATGAAAGCAGTTTTAGGAAGTACCGTTGGTTGTGCTGCTACAAAAGCTTGGGTAGAGTTGGGTAAGCCTAATTTGCTTGGTGCGACAAATTGTTTAACATCAGTGTATTTGCCAATTCTAAAATCAACGGTTCGCACAAAATTGATATCAAATTGGTTGTAAGGCGGATGATCTTCTCGGTTTTTTAAGGCCTGTATGACGTGTAAATTAGTAGCTAAGTAAACAGTAAATTTATTAGCTTTTTTATCTTTCTTGTCAACTTCCTTCCAGTCAATTAACCAACCCGTTCCGTGAATGGAACCAAAATCTATTTGACTTTTATCTTTCTCGGTTTTTTCAATTCTGTTAAAAAAACCTAAAGAAAAGGTTAAATCATAGTTGGCATGATCGATTTCTGCACTACCAGGAATATTGTTAATGTAATACCCTTCGAATTTATCAGGTTTTCGAAAGGCTGAACAAGCACTTAAAACAGTTAGTGCCAATAATCCACTAAGAAAACAAAAACGCTTGAGCGCAACTCACATAAACGAGTAATTCTAATAAAGGGTTAAGGAGCCCTGTTTGCAGTGATATCCCAAAGGATTAAACCACAAAATTTTTAAGACTTTTGGTTTTTAACAAAGGTAAATTGTTGGTCGTTGCTTTGCTTAATTTGCTCAAACAAATGGGTTTTATGTTGTTTAGCAAATTGAGCGTAGTACTTTTTAGTGTCTTTGTTACCAAAGATTAAGTCATAGGCCACTGTGTTTGTGCCATCGTTATTATGGCCATTGGCTTTTCAACGCAGCAACTGAGCTACCCCTCAAGCAGTAGTGGTGTTACTAGTAGACGCAAAATAAATGCCAGTAATTTGGTGCTTATCGTTTAACACTAAAGCACCACTAGCGCCACTTGTAAAACCAGCATTGTCCAAAACAAATCCCTTACCGTATTGTTGGTAATTACGACTTTGAAAAGTTTGTTCAAACCGAAATACCCTACTGTTGTCATAGTTTCAAACCCAGTTACTGCCATTAAAGTCATCACCTTGATTTCTAATTAGGGTTGGCTTTGCTATTGTCGATCGCACAATAAGGGGTTGTTCGTGGTTACTTTGTTGTACTGTTTGTCCATTTATTGTTGTTTCTGTAGATGTATCTTTCTTAATTTGATTAACACTCAATACATTAACCTTGTTTTCCAAGAAGGGATAACCCGATAAATAATAGCGGTGGTTTTTGAGTTCAGTTAATGTTGGCTTGGCAAACAGCTTTAAGCTATTACCTAATTGCTCATAAGTTTTGATGGCTGGTTGGATAAAATGGTCAAACACCTTTTTATCAACTGGGTTATCTAAAAATAAGGTTAGCTCTAACACCGCAAAGTCAGCATAGGCAAGACTGTCTGATGTATTTGCTGTATTAGATTCCTTTTTATCATCAGCATCCTTTTGTTGAATTCACTCAAACGGTTGGGTTTCCGCTTGCTCCTTTTGCTTATCGTTTTCTCCATCCTTTGGTTGCTTCTTTTTATTTTCTGTATCATTTTGCTTCTTTTTGTCTTTTGGCAAAGTGTAGTTGACAAAGTCATGCGCTGTAAAAGCGGTTTTGGGAATTACTGTGGTTTGGACATTAACAAAGGCTTGAGCATTATTGGGCAACTTAACTTGACTAGGTTCGACAAAATCACGAACGTGAGTGTATTTGCCTATTCGAAAAGCGGTCGTTCAATCGGCACTGGTATCAGAGTTTTTATAAGGATCGTAATCCTGTTGGTTTTTTAAAACGGCTGCGACATGTAAGTTGGTGGCTAAATAAACCCGAAAGGGTTCATTTAACCCCCCCCCGCTACCTTTTCAATCAATGAGCCAGCCAGTGCCAGAAAAGGCGCTAAAGTTATCTTGCCCATTGTTAGTAGTTTTATTAGTAAAAGTTAAAGCAAAGGTTAAATCATAGTTGGCACGGTGAATCGAACCACTACTAGGAATATTACTTACATAGAACTGATCCCACTTTTCTAGTGGCACTGCAGCACAAGCACTTAATACAGTTAAGGAAGCTATGCTAGCGAGAAAACCAAGGCGCTTGAAAGCCACAATCATAATGGTGTTAATTCTAATAAAGAGTTAATTAGCTACTTTTACAACTAGCCACTAGTCGATTCATTACCCCAAGTTATGCCTTTTAGTCTTTTCGACAAAGCGGAATTCATCATTGTTAATTTGCTTAATTTGCTCGTATAAATGGGTTTTGTGTTCTTTGGCAAACTGAGCATAGTATTTTGTGGTATTTTTATTACCAAAGATTAAATCATAAGCCACACTTCTAGCTTCATCATTGTGATAAGTTTCTGGTTTTCAGCGTAATAACTGTGCTAAGCCTAATTCATTGTTAGCACCATCAACGCCAAAGTAAATGCCAGCGATTTGTTTCTTATCATTTAACAACAAAGAACCAGAAGATCCACCGCCTAAATCAAGATGACTAATGCCTATACTTTGGCCATAGATTCGGTACTTTTTGCCCGAAAACTCAAGATAAACCTTAAATTTCTGGGAGAGATCATAGTTCAAAGCTCAGGGACTACCAGTAAAATTATCGCCTTTATTCCTAATTAAAGTGGGTTTAACTTGATTAGTTGAGATAATAGCTGGTTTTTCATGGGATATTTGTGTATTTTCGCCTAAATATTCCTTTCTTTTTTCCCGTTTGGTTAAGAAATAGTGATGACACTTTATTTTTTAAAAAAGGATAACCTAACACATAATAGCGATTTTGTTTTAATTGATCCAGTGTGGGGTTAGCAAAAATGTTAAGGCTATCACCTAACTGTTTATAAGTGCGTATGGCTGGTTGAATAAAGTGATTAAAGATTTTGCGATCTACTTTACTTTTCGAAAATAAGGGAATTTCTAAAATTGCAAACTCAGCAAAAGGCTGGACATCAGAATTCTTAGTATGACTGTTTTTCACCCATTGTTGTTTTCTCTTTTCTTTATCTTTTTGTTCTTGAGGAAAACTGTAATCCACAAAGTCCCTAGCAGCAAAAGCGGTTTTGGGAATTACTGTGGTTTGGACATTAACAAAGGCTTGAGCATTATTGGGCAACTTAACTTGACTAGGTTCGACAAAATCACGAACGTGAGTGTATTTGCCTATTCGAAAAGCGGTCGTTCAATCGGCACTGGTATCAGAGTTTTTATAAGGATCGTAATCCTGTTGGTTTTTTAAAACGGCTGCGACATGTAAGTTGGTGGCTAAATAAACCCGAAAGGGTTCATTTAACCCCCCCCCGCTACCTTTTCAATCAATGAGCCAGCCAGTGCCAGAAAAGGCGCTAAAGTTATCTTGCCCATTGTTAGTAGTTTTATTAGTAAAAGTTAAAGCAAAGGTTAAATCATAGTTGGCACGGTGAATCGAACCACTACTAGGAATATTACTTACATAGAACTGATCCCACTTTTCTAGTGGCACTGCAGCACAAGCACTTAATACAGTTAAGGAAGCTATGCTAGCGAGAAAACCAAGGCGCTTGAAAGCCACAATCATAATGGTGTTAATTCTAATAAAGAGTTAATTAGCTACTTTTACAACTAGCCACTAGTCGATTCATTACCCCAAGTTATGCCTTTTAGTCTTTTCGACAAAGCGGAATTCATCATTGTTAATTTGCTTAATTTGCTCGTATAAATGGGTTTTGTGTTCTTTGGCAAACTGAGCATAGTATTTTGTGGTATTTTTATTACCAAAGATTAAATCATAAGCCACACTTCTAGCTTCATCATTGTGATAAGTTTCTGGTTTTCAGCGTAATAACTGTGCTAAGCCTAATTCATTGTTAGCACCATCAACGCCAAAGTAAATGCCAGCGATTTGTTTCTTATCATTTAACAACAAAGAACCAGAAGATCCACCGCCTAAATCAAGATGACTAATGCCTATACTTTGGCCATAGATTCGGTACTTTTTGCCCGAAAACTCAAGATAAACCTTAAATTTCTGGGAGAGATCATAGTTCAAAGCTCAGGGACTACCAGTAAAATTATCGCCTTTATTCCTAATTAAAGTGGGTTTAACTTGATTAGTTGAGATAATAGCTGGTTTTTCATGGGATATTTGTGTATTTTCGCCTAAATATTCCTTTCTTTTTTCCCGTTTGGTTAAGAAATAGTGATGACACTTTATTTTTTAAAAAAGGATAACCTAACACATAATAGCGATTTTGTTTTAATTGATCCAGTGTGGGGTTAGCAAAAATGTTAAGGCTATCACCTAACTGTTTATAAGTGCGTATGGCTGGTTGAATAAAGTGATTAAAGATTTTGCGATCTACTTTACTTTTCGAAAATAAGGGAATTTCTAAAATTGCAAACTCAGCAAAAGGCTGGACATCAGAATTCTTAGTATGACTGTTTTTCACCCATTGTTGTTTTCTCTTTTCTTTATCTTTTTGTTCTTGAGGAAAACTGTAATCCACAAAGTCCCTAGCAGCAAAAGCGGTTTTGGGAATTACACTTGTTTGCACATTGACAAGAGCTTGCGCAGCATTGGGTAAACCAAATTGATTGGGTGAAACAAAATCACTAACCTTAGTGTATTTACCTAACCTAAAGGTAGTAGTTCAACCAGCATCATCTTTATTTTTATAAGGTTCATAATCTTGCGGATTTATTAATGATGCTGCTACTTGTAAGTTAGTTGCTAAATAAGCACGAAAGGGATCATTCTCTTTCTTTTTATCAGGTTCCTTTCAATCAATTAATCAACCAGTACCATATGTGACACTAAATTTAGCTTCACCGTTGCTAGTGATTCTATTAGTAAAAGTAAGCGAGAAGTTTAAGTCATAGTTGAATCGGTGGATTTCCGTACTACTAGGGATATTGTTAATGTAAAACTCTTGCCACTTATCAAAGGCTGCACAAGCACTTAAAAAAGTTAAAGAGAATATGCTAACAATAAAACCAAGACGCTTGAAAACAACCAACATCGTGGCTTAATTCTAACAAAGGGTTAAGGAGTTAATTTTTAAGCAAAATCCCAAAGGTTTTAACTACAAATTTTTAACACTTTTGGTTTTTGACGAAGGTAAATTGTTGATCATTGCTTCTATCAATTTGTTCGTATAAATGGGTTTTATGTTCTTTAGCAAACTGAGCATAGTATTTTGTGGTATTTTTATTGCCAAATATTAAATCGTAAGCAACACTGTCTTTTTCTTCATCATTATTTTTCGCTTGTCAACGTAATAACTGTGAGAAGCCTAATTCGTCTTTAGGGCCATCAACACCAAAATAAATACCAACAATCTGCTTCCTATCATTTAACAGCAAAGAACCAGAAGAGCCACTGCTTAAATTAAGGTCACTAATGACTATACTTTTGCCATAGATTTGGTATTTCTTACCTAAAAATTCATGATAAAGCCCCGATTTCTTGCTGAGATCATAGTTTGAAGTTCAAGTGTTGCCAGTAAAATTAGCGCCTTTATTCCTAATTAAAGTAGGCTTAGCTGGATCGGTTAAAACAACAAATGGTTTTTCATGAAGTATTTGGGTAGTTTTGTCCATATGTTCCTTTCTTTGTTCCGTTTGATTTAAAAACAATGATGCCGCTTTATTTCTTAAAAACGGATAACCTAACGCATAATAGCGATTTTTTTTAATTGATCCAGTGTGGGGTTAGCAAAAATGTTAAGGCTATCACCTAACTGTTTATAAGTGCGTATGGCTGGTTGAATAAAGTGATCAAAGATTTTGCGATCTGTTGGATTATCCAGAAATAAGGGAATTTCTAAGACCGCAAAATCAGAGTAAGACCGGGTATCAGAATCCTTAGTCTTACTGTCTTTAATCCATTGATATTCTTCCTTTTGATTATCTTTTTGTTCTTTAGTAAAACTGTAATTAACAAAGTCCCGAGCAGCAAAAGCCGTCTTGGGAATTATCGTGGTTTGGGCTCTAACAAGGGCTTGCACAGCATTGGGTAGTCCAAATTGATGGGGTGCAACAAAATCTTTTACGTTAGTGTATTTACCTAACCTAAAGGTAGTAGTTCAACCCAAAACAGTATCTTTATAAGCTTCATAATCTTGTGGATTTATTAATGATGCTGCTACGTATAAGTTCGTGGCTAAATAAGCGCGGAAGGGTTCATTCTCCTTCTTTTTGTCAGGTTCCTTTCAGTCAATAAGTCAACCAGTACCATATGTAGTAGCTATGAATATAAGTTTTGTCATTATTAATGTACTTATTAGTAAAGGTTAAGGAAAAGCTTAGGTCATAGTTAGCGCGGTGCACTTCGCTACTACCAGGAATGTTATTAATGTAAAAATCACGAAATTTATCGGGAAGTTTAAAGTTGCACAAGCATTTAAAAAGGTTAGTGCCAATACGCCACCAAGAAAACCAAAACGCTTCCAACTAATCCACATAACTAATTCTTTTTAGCTTTCTCCACAAAACGGAATTGCGGGTCGCGGGTTTTAGTAATTTGCTCGTACAAATGGGTCTTTTGTTGTTTGGCAAACTGAGCATAAAAGTGTTTTGTGTTACTGTTACCAAAGATTAAATCGTAAGCCACACTACGAGTCTCATCACTACTGTTTTTGGCATTTCAACGCAATAATTGGATTACCCCCCAATCACCATTGGCAGTGAAATGGGTAGCAAAGTAAATGCCAGCAATTTTTTTCAGATCATTTAAAACAACGGTACCAGAAACTCCACCAGTAAAACCAGCATGGTTTAAAACAATTCCCTTGCCATATTGTTGATAATGACGTCCTTGAAAATTTTGGCCAAATTGAAAACCAGCCACATGATCATAACGTCAAGCAACTGTGCTACCATTAAACTTATCGCCCTTATTTCTAATTAAGGTAGGTTTAACCGTACTCTTACCCACAACAAAGGGTTTTTCAGTTTTACTTTCCTGCGTTGTTTGCTGAGGTATTGTTTTAGCTGCTGCTATGCTTGCTTTAGTTTGGTTTACCAGTAAAGTTCTAACACTATTTCTTAAATTGGGATAACCAAGTACATAATAGCGGTTTTGCTTTAACTGGTCTAAGGTTGGATTGGCAAAGATGTTTAAACTATCACCTAACTGTTTATAAGTATTAATGGCCGGTTGAATAAAGTGATTAAAGATTTTGCGATCAGTGGTTTTATCAAGAAAAAGTGGCATTTCTAATACCGAAAAGTCAGCATAAGAAAGGCTATCTGTTGTATTAGACTTAGTCCCTCTTACTCAATGTAAATCTGTTAATTCTTCTTTTTTATCTACTTGATCTTTGGGCAAACTGTAATCGACAAAATCCCTGGAAGTAAAAGCTGTTTTGGGTAGCACCGTTGGTTGCGCAGTTACAAAAGCTTTGGCGTCATTAGGTAAGCCTAATTGACTAGGTAAAACAAAATCTTTGACACTTGTGTATTTAGCAAGTCTAAAAGCTTTGGTAACACCATATTCAGGATCTAATTTATTGTAAGGTGAATAGTTACCTAAAGTTTGAAGATTATCACCTAAGTGCAAGTTAGTTGCTAGATAAACACGGAAGGGATCGTTCTTTTTATTTTTGTCTGGCTTCTTTCAATCAATTAATCAACCACTGCCATAAATTGCAAAATCTTTAGTTTTGTTTTCAAGGTTATATCTATTAGTAAAGTCCAAGGAAAAAACTAAATCGTAATTAGCACGGTGGATTTCCGTACTATTGGGAATATTATTGATGTAAAACCCTTCAAATTGATTAGGTAATTGAACTGTTGCACAAGCGTTTAAAAGAGTTAAAGAGGCTATGCTAGCTAGAAAACCAAAACGCTTGAAGGCCAATGCCATAGTTACTAATTGTAATAGACAGTTAAACTGGTACTTTTAAGCTTAAACGCTAAAAAGTTAACGGGTTGCCATTGTTAACTCTGTTTAATTTCCACAAAGCGGAATTAAGGATTGGGCGTTTTAGTGATTTGTTTATACAAATGGGTATGGTGTTGTTTGGCAAACTGAGCATAGTGCTTAGTGGTATTGCGGTTACCAAAGATGAGATCATAAGCAATGCTCAAAGCTTCATCATTGCTCTTCTTCCGTGGTGTTCAGCGTAACAGTTACGCTAAACCCCAAGCCTGTTTAGAAGAACGGGTAGTAGCAAAGTAGATTCCCACAATTTGCTTTTGGTCATTTAAGACTAATGCACCTGACACACCACCACTAAAATCAGCATGCACAACTGCCAAACCCTTACCGTATGCTTGGTATTGTTTGTAAGTATACTGGTGCTTAAACTTAAAGCTCTTACTGTGCTCATAGTTTCAAGCAAATTCACTTTGATTGAAAGTTGCACCTTTAGTGCTAATTAAGTTGGGTGTACCACGTCCTGAATACAATAAAAAGCGGTTTTGGTTAGTTTGTTGCAGGATCTGTTCGGGTTTTATTTGTTTTTTACCAGTTTGGTTAATTAGTAAGGTTTTCACCTTGCTCTGTAAGTTGGGATAACCCAATAGATAATAGCTGTGGTTCTTGAGCTGATTAATTGGGTTATCAACAAAGATGTTTAAACTATCACCTAACTGTTTATAAGTGCGTATGGCTGGTTGGATCAAATGATCAAAAATGTTGCGATCAGTGGGATTATCCAAAAACAGTGGTAGTTCTAAAACTGCAAAATCAGCATAGGGATGGGTAACTGTTTTATCAGATTGATTGCCTTGCTTTCATGCTTTAGCATCTTTAGGCAAGGTGTAATCGAAAAAGTCTCTCGCTAAAAAAGCCGTTTTGGGAATTACAGCAAGTTGGGTAAAGACAAAGGCTTGCTCAAAATTGGGTAAACCTAATTTGCTTGGTAATACAAACTGCTTTACATCGGTGTATTTGCCCAAACGAAAGGAAATTGTTCAACCTAAAGCAGGATCGGGTTGGTTGTAAGGCGAATAGTCTTTCGGATTCTTTAGTGATGCTAATACGTGTAGGTTAGTCGCTAAGTAAGCGCGGAAGGGTTCTGTTTTACCACCTTGCTTTTCACCCTCTTTTCAGTCAATTAACCAACCCGTACCATAATTAGTCTTTAACCAGGTTATCCCCTTTTGGTCGGTTTACTTATTGGTATAGGTTAAAGAGAAGTTTAAATCATAGTTAGCACGGTGTACGTCTAAAGAACTGGGAATGGTGTTGATAGAAGCCACCCCCCCACTGCTCTCGTGGGTTCATAACTGCACAAGCGTTCAAAAGGGTGAAAGTGGCTCAACTAGCCACAAAACCGAAACGCTTGCGAGCAATTACCATAATTGGCCTAATTCTAATAAAGAGTTAATTGGCTAATTTTTGATCTAAAAGTCACTTAAGAGTTTTTAACATCCGTTGTCTTATCACTTTGTACGTATTTCAAACTTTGATCCTTTGCTTGTTCAATTTGCTTGTAAAAATGGGTACTGTTTTTCTTAGCAAATTGGGCGTAGTACTTGGTGGTGTCCTTATTACCAAAAATTAAGTCGTAAGCCACAGCTGAGTTTTCACCAGTTTGATTGCCCATTGCTTTTCAACGCAACAATTGGGCTACTCCCCAAGCATCAACGTCCGAATAAGTTGTACCAAAGTAAATGCCGGCAATCTGTTTCTTGTCATTGAGCACTAAAGAACCAGAAGCGCCCACTTCAAAACCGGCGTTAGTTAAAACTAATCCTTTACCATATTGTTGGTAATACTGGTTTTGGAAAGTTTGACTTAACTGAAAGATCTTGCTGTGGTCATAATCTCATGCCAATGTACTGTCATTAAAATCATCGCCTTTATTGCGAATTAAGGTTGGTTTCATCAAAGAAGGACGAATATCAAACGGTTTGTCGATGTTACTGTGCTGTACTATTTGGTCGTCTTCTTTTTTGGTTTTATCTGTTTGGTTAATAAACAAAGATCTAACCTTATTTTTTAGATAAGGATAACCTGCCAAATAATAATGGTTACCTTTCAGTTCATCTAGTGATTGTTTAGCGAATAGATTTAAGCTATCACCTAAAAGCTTATAAGTTCTCATGGCAGGCTCGATGAAGTGATCAAAAACCTTTTTATCATTTTGATTCTCCAAAAATAAGGGCAGTTCTAAAACTGCAAAATCGGCATAGGCAAAACCTTCGCCCGATTGTGATTTATCTTGACTGCCTTTATCTTTACTATCACTTTGTGATTTGGCAATTTGAACATTACTATCATTTTTCTTTTGTCATTGAATTGCTTGCGTGTCAGTTTGTTGTCCTTCTTGTACTTCTCCTTTTTGTGCTTTTTCGATAAGTTCTTGTAATTGCTTCTTTTGTTTTGTTACTTCATCTGCTGACAATTTGTAATTCACAAAATCTCTAGCAGTAAAAGCAGTTTTGGGAATTACCGTTGTTTGCACATTGACAAAGACTTGGGCATTGTTCGCTAAATTGACTTTACTTGGCAACACAAAGTCCTTTGCATTCGTGTATTTACCTATGCGAAAGGCAGTGGTTTCTTCTTTGTTATCTGTTTCTTTATTGTATGGCTCATAGTCTTTCTTATTTTTTAAGCCATCAGCTACGTGGAGGTTGGTTGCTATATAAGCACGGAATGGTTCATTTTTACCATTTTTTTGTCCACCTTCTTTTCAGTCAATTAACCAACCAGTACCATAGGTAGCAATGAATTCTGTTTGATTATTTTTACCGTTAAACTTGTTAGTAAAAGTTAAGGCAAAACTAAGATCATAGTTAGTGCGGTGTATTTCTGCACTACTGGGAATATTATTAACGTAAAACCCTTCAAATTTATCAGGCTTAAAAGCAGCACAAGCACTTAAAAAGGCCAAGCCACCTATGCTAAAAATGAAACCGAAACGTTTAAAAGCAACCAACATAATTAGCTTAAATTCTAATAAAGAGCTAATCTGTAATTTTTAGTCTATGCGCTAACATGCCATTACTCGTATTTATTAGTTATTGGAGTTTTCCACAAAACGGAACTCAGCGCTCCTACTTTTAACAATTTGTTTGTACAAGTGGGTGTTATTTTTCTTTGCAAACTGGGCGTAATATTTTCTCGTGTTTGGATTGCCAAAGATGAGATCATACACCACACTAACTGCCTCTTCATTAAGACCACTACGCGTTCGTCAACGTAATAACTGTGCTAGACCTCACTCATTTGGCCCAGAACTACTGGCACCAAAATAAATACCGGCAATCTGCTTTTTATCGTTTAAAACTAAAGATCCAGAAGCGCCACCACCCAAATCACTACGATTAATCCCTATGCCTTTGCCATAGATTTGGTAATCTTTACCCAAAAATTTGCGATTAAAGGCAAAGTTCTTGGTGTGATCATAGTTTCAAGCTCAAGAACTACCATTAAAGTCATCACCCTTATTTCTAGATAAAGTAGGTTTAGATGGGACTCTTAAAGAAACGATGGGGTCTTCATTGGGCACTTGTGCCTTTTGACCCAAAATCTCCTTTTCTTCACCTGTTTGACTTACAAATAAAGATTGACCCCTTTCTCTTAAAAAAGGATAACCCAACAAGTAATAACGGTTATGTTTAGGTTGATCTAGGGTTTGATTAGCAAAGATATTTAAGCTATCACCTAATTGTTTATAGGCTCTAATGGCAGGTTGGATAAAGTGATTAAAAATTTTGCGATCAGTAGAACTGTCAGGAAACAAAAGGATTTCTAAGACTGCAAAATCAGCATAGGGATGAACATCAGTATCCTTAGTTTGACTGTTCTTCATCCACTGCTGCTCCTTTTGTTCTTTACCTTTTTGTTCTTGGGGCAAGGTGTAATCCACAAAATCGTGCGCAGTGAAAGCCGTTTTGGGAATTGTGCTTGTCTGCGCTGATACAAAGGCTTGTGCACCATTAGGTAATCCTATTTCGTTTGGTGACACAAAGTCTTTCACGTTAGTATATTTGCCCAGTCTAAAATCCACCGTTCAACCCAATTCTGCATCAGGTTGACTGTAAGGTAAATAGTCTGCCTCATTCTTTAACCCTGCAGCTACATGAACATTAGTCGCTAAATAAACCCGGAATGGTTGGTATTGATTCCCCTCCTTTCAGTCAATCAACCAACCAGTACCATAAAAGGCAACGAAGTTTACTTCATTCTTCTTTTTAGTCTGAATTCTGTTGGTAAAACCTAAAGAGAAGGTCAAGTCATAGTTAATCCGGTGTAATTCAGCACTACTAGGAATATTGTTAATGTAAAACTCACCAGATTTATCAGATAGTGAAATTGTTGCACAAGCGTTGAGCACAGTTAATGTAGCTATGCTAGCTAGAAAACTAAACCGCTTTAAGGCAATTACCATGGTTGCTAATTCTAATAAAGAGTTAATCTGGCACTTTTGGGATTAAATGCCAGCTGATTAATTGCCTAAATTATTAGTTGTCTCTTGTGCTTCCACAAAGCGGAATTGTTCGTCTTTGCTTTCTTTAATCTTCTGATACAAATGAGTTTGATGATCTTTAGCAAACTTAGCGTAATAATTCTTGGTGTTCTTATCGCCAAAGATCAAGTCATAGCTACCCTTAGAAATACTTGAACCTTTATCGTTCTGTGGAGTACGTAAGATTTGGCTCATCCCAATGTCATTGCGTGCAAGACTACCAGATTGGTAAATTTCCAACACCCCAAAGTAAATGCTGTTCAATTTATTGTCTTTACCAATTAAAGGACTACCCGAAGAGCCACCCGGAAAATTAGTGTCATTCAACATTAAGCCATAGCCGTATTGTTGGAACCGCTTTTCGTGGTACTTCAAGGTAAAGTTGCTAAAGGAAGCAAAGTTCACCACACCTTTGTCTTGGATCACTACCCCATTAAGCCAGGACTCGTTTTGTTGGTTGGCAAAGAGTTGACTACCAGTGCTTTCATTGTAAGCACGGTAATTGTTAGTTAGGACGGGAATCCTGTCTAACTTATTAATATAGCTTTGATCTTTTGTAAATCACTGGCTCTTTTGGTTATGATCCGAATTGTTGTGATCAAAGCTCTTTTGTTGCGCTAATAAAAGGTTAGCGGTATTGTTTCCTTTATTAATCACTGGATAACCTAACAAGTAGTAGTTTTGTTCGATGAACTGGTTGAGTGGTTTGGCACTAAACAGTTCCAAAGTATCACCTAAAGCCTTGTAAGCCTTAATTGCTGGTTTGACAAAGTCATGAAGAATTTGCTGATCGCGTTGATTTTTAAGGTTTAGGTTAACTTCAATCACGGCAAAGTCAGCATAGTTGCCAGGAAGACTTGTTGTGATGTTGTTATAACTGTAACTATTCATATCAACAAAGTCAGTAGCAGTGTAAGCCGTTTTGGGAATATTGGAGGCCTGAATCGAAACCAAGGCTTTATCAGTTTGATTGGCTGATTGATTTTCTTCTTTAGAAATGTACTGCTTTAACTGCTCACCTTCTAAGTATTTACCCAAAGAAAAGAATTCAGTTTTAGTATTCTCAGTAAAATCTTGCTTGTTCTGATCTTTTTTGTAGGGTTCGTAATCCTGATCATTCCTTAAAGCATCAGCTACGTGTAAGTTAGTGGCTAAATAAACGGTGAATTGATCATCCTTTAGAAGGGAGTTATCAATGTTGCTTGTAATGCTAGGGACAAAACTGCCATCAATACCTAATGCCTTTTCATCACCCTTCCAGTCAAATAACCAGCCGGTGCCATAGATATCGTTAGACTTATTGGTAAAGTTCAACGAAAAAGTTAGATCATAGTTATGCTTAAAAACATCACTTTCACTAGGTACCTTATTGGTTTGGAAGTGAAAGCCACGGTTAACAGGCACTACCTCTGCGGTTGGTTTAGGTACATCAGTTGTAGGTGTGGTAGGTGTAATGGGACCACTTTCTTCAATGTTATTGGGTTCAAAGTTTGGCTGGGTTGCAGCACAGGCACTAAGAATAGTGCTGGTACCCAGCAGTGTTAAGAAACCCAATAACTTGAATTGCAGTCTCATAGACTAACTATTTTAATACAGATAGCCAAGGAGCTAAACCATTATAGCCCGGTTTATCTAGTAAATAAAGAGGCACTTAAACCATCAATGATTAATTGAATCAAGGATCTTTTTCGATATAACGGAACTGTTCATCAGTACTATTTTGAATTTCACTATACAGGTGGGTTTTATTTCGTTTAGCAAACAAAGCATAATAGTCTCTATTATTACTATCACCAAAGTATAAGGTCGTAACTGTATTTAACTAAACTTACCCCATTAACATCTTTAGGAACAGGCAATAGCTGGGCATAGCCAACACTATCTGATTCAGTAGCAGCTGAATAAATACTGCTAATCTTTTTGTCTTGGTTAAACACTAAACTGCCACTCGATCCACCTAGTAAATCAGTATCAGTTAGAGCTAAGCCATAGCCGTGGTTTTTATATTCATATCCATGATAATCAGTGATAGCACTTTTAAAAGTACCAAAATCAACAATTCCTTGATTTAAGGAATATTCATTAAGGAAGCCGTTACTGTAATATGTGTGCACTTTACTACCAGCAACTGGTTGACCTTGCCTTAAGTTTTTGGTAAGTGTTGGCATAGAACCATAAAAGTCATCATGGTTAAAACGTTGTTCTGTTTTGGTTCACTCAAACAACTTAGCATCATCAGTCCATAAAGTTGGATAACCCAAAAAGTAATGGGTATCGTTTTGGTATTGGGTTAATGGTTAACTGGCGAATATACCAGTACTGTCACCCAATGCTATATAAGCTTTCATCGCCGGATCAATAAAGTGATCTTTAATCTGCTTATCTTGCTTATTACTTAAATCTAGATTGATTCCAATACAGCAAAGTCAGCGGCAGGTAATCTATATTCCCCCAAATCATTAATAAAAAGTGTTGCCGTGTAAGCAGTTTTTGGCAATTTTGAAGTTTCAATCCGCACTAAGCTAGGTTCATGCATAGCATCAGGAACTATTTGCTTTATTTCATTACCATCAACGAACTTACCTAAAGTGAAAGATTCAGTTGGATCGTCAAAGTAATCAAATTGATTGAAAAACTCATAATCATTCACATTTCTTAAATTATCAGCTACATGTAAGTTAGTAGCTATGTAAGCAGTGAATTTATTGGGCTGATTATTATCCTTTCAATCAATGAGCCAACCAGTACCGTAAGTAGCTGTATATGGATCTTGAAACTGGAGCGTAAAACTTAGATCATAAAAGGGACGAAAGGGATCATCCTTTTTTGGAATGAAGTTAGTATTGAACTTTAAGTCAGAGGTGTATTCACCTAAAGCAGGGTCTGGTTTTAATGCTTGCTTGTCTTCTGGTTGAACTGCACTAACACTTTTGTTGTTTACAGGCACAGCCGGTGCACAAGCAGTAAAAACTCCTTGAACTAGTAAAAGTGTTAAAAAGACAAAACCTTTTAACCTATATCTAAGAAAAAGCGGGGTTGCTTTAGTAATAGGCAAATTAAAAAAACTTAAACAAATACGAAAAATTAATGAAAAAGCTAACTAATGGAGCAATGCTCGATTATATAAGCTTGCTTTAGTGTGGGTTGGTTAAGTTGGTCGATTTAACACAAAGCTAATGAAAGTATGCTTAGCTTTTTACCGGTGTTTAACTTAATTGAATTGTTTGTCTACCACATAACGGTACTGTTCATCAGTACTTTTAAGGATTTCACTGTACAAGTGAGTTTGCTGTTTTTTAGCAAACATCGCATAGTAACGCTTAGTGTTGCTATCACCAAAGATGAGGTCATAGCTTTGTGAAACTACACTTATACCGTTGACATCCCTAGGAGTTCGTAATAACTGAGCATAGCCAACACTATCTGATTCAGTAGCAGCTGAATAAATACTGCTAATCTTTTTGTCTTGGTTAAACACTAAACTGCCACTCGATCCACCTGGCAAGTTAGTGTCGGTTAAGGTTAAACCGTAACCCTGTTGACGGTAATCGTAATCGTGGTAATTAATGTTAGCACCCAAAAAGGTACTAAAACGCACAATACCTTGATCCATTGAGTCCTTATCGAGTAACTCATTGCTGTATTCGATGTGTGTCTTACTACCAGCGTTTGGATCGCCCTGCCTTAGGTTTTTGGTTAGTCTAGCCATATTACTGTAAAAATAACCATAGCTAAAGCGCTCTGCCCCTTGTTTGCATTCCCACAGTTGTGTATGATCAGTAGGTACAACCGGATAGCCCAAGAGGTAATGGGTATCGTTTTGATATTCAGTTAATGGTTTGGTGGCAAACAAACCGGTACTATCACCTAATTGTTTATAAGCCTTGATCGCTGGATCGATGAAATGATCTTTAATTTGCTGATCCTGCTGGTTGTTTAAATCCAGTTCTAACTCTAATACGGCAAAGTCAGCGGCAGGGATTTTGTAATCAAAATAATCCCAGTAATATTTTTGGTCGTTAAATAAATAAGGAAAACTATAATACTCGCTTTCAATAAAACTTGTAGCTGTGTAAGCTGTTTTGGGTAGCTGTGAAGTTGCAATACTGACCATGCTAGATTCACTAAAGGCATCTAGTGCCATCTGCTTAACTTCAATCGCATCGACAAACTTACCTAAAGCAAATGATTCGGTTGGTGCCTCTAGATCAAAATAACTGGGGCGGTATTTAAAAGGATCGTAATCATTAACGTTCCTTAAGCTGTTAGCCACATGTAAATTGGTCGCTAGGTAAGCGGTAAATTTGTCTTGGGAATTATGATCTTTTCAATCAATTAGTCAGCCCGTACCAAAAGCTAAACTTGCTTGGTCTTGAAACCTAAGCGTAAAACTTAAATCATAAAAGGGACGAAAAGGATCATCCGCTTTAGGAATAAAGTTAGTGTTGAACTTTAAGCCAGGGGTATATTTACCCGAAGCTGGATCTGGTTTTAATGCTTGCTTGTCTTTTGGTTGAACTGCACTAACAACTTTGTGATTGACAGAAGTAACCGGAGCACAAGCAGTAAAAGCAGTCTGTACCAGGATAATTGTTAAAAAGGAAATACCTTTTCACCTAAACTTGGAAAAGAGTGGAGTTATTTTCAGTTAGGACAAATTTCAAACAAATACTTAAAAATACACGAAATACCAATAAAACAGCGAAGCTAATTTAACAGTGCCCAATTATATAAGTTTGGCATAAATGTACTTAGGTTAAGCTGGAAAATTAACCCTAGCCCTCATTAAGTTGGCTTAGCTTTTAAATGGTGTTTGACTTGTTACAGTTTGATCGTTTTCGACAAAGCGGAACTGTTCATCGTTACTTTGAAGAATTTGATGGTACAAGTGGGTTTTGTGCTCCTTAGCAAATTGAGCATAGTATTTCTTGGTGTTGCTATCACCAAAGATTAAATCGTAGTTTTTGGACTTGTCTTTGTTTTGCGTAGCGCGGAGAATTTGCGATACACCGTAACTTTTACTTGGTAAAGCCGCAAAGTAAATACTGTTAATCTGTTTGTTTTGGTTAAATAACGGACTACCACTGGAACCACCCGGGAAGTCAGTGTCTTCTAACAATAAACCATAACCATGGTGTTGGTATTGCTTTTCGTGATAGTTAAAAACGTAATTGAAATACTTGCTAAAGTCCATTACGCCTTTAGGGTCATTTTGTGCAAAGGGTAAATAGTTAATTTGATTTTGTACCAAACGACTACCTGGAATTTCATCATCTTTCCGGATGTTACGCGTTATGCGCGAAACTTCCTTATTACTATTTTCATAGAGTGGTGAACTATAACCCTTCTGTGGTAAGTTTCAGTAAGACGTCTTTTCACGTTTAAGTAACGGATAACCCAATAGATAATGGGTATCATCAACATACTGATCTAATTGCAGGTTGGAAAAGAGACCCAAACTGTTACCTAGCTTTTCGTAAGCAGTTACCGCTGGATTTATAAAACTGTCAAAGATTTGCTGGTCACGATTGTTGGCTAAATTTAACTCTAATTCCAACACCGCAAAGTCAGCGTAACTTACGGCATTTTGGAATAAGTCCAACTCATAAACTGTGTTACCCACTATGTGATTGTATTTGTACTGAGCAGTTTTGATAAAGTCAGTGGCAGTGTAAGCAGTTTTTGGTAACTTCGATGTTTGAATTGAAACTAATGCATCACTATTCCGGTTATTGAGGTGCTTAAGATTTTGGTACTCTGGAGTTAACTTTTGCACAGTTTGGGCATCAATGTATTTACCCAAAGAAAATTCAGTGGTGATATCGCCAGGTAAAAACTCCTTCTGATTACCATCCTTGTTGTAAGGCTTGTAATCGTCTTTATTTCGTAAGCTATCAGCTACATGGAGGTTGGTAGCTAAGTAAGCAGTAAATTTGTTGTCCTGCTTAACATCCTTCCAGTCAATTAACCAACCTGTACCATAAGCTTCTTTTCAACTAGTACCAAACTTTAAGGCAAAGCTAAGATCATAGCCCGGCCGATACTTAGTATTTACTTTAGGAATAAAGTTAGTGTTGAACTTAAAACCAGGACTGTAAACACCCTTGGAGCTGTCAGGCTTAATTTGCACCTCGGCATTCTGCACAGGTGGTGTAGCAACAGCATCGGGCTCTATAACTGGTTCAGCCTCTGGTTTGGGGATAGTTTGGCTCAATGGTGGTTGAGGTTCAAAAATCACAAACTTATCACTTTCATTTTTGTGATTAGTGTTAGCTACTGTTAGCGTAGCAGAGCAAGCAGTTAGAAATGCTTGACTGGCGAACAGTGTGAGAAAACCAAAACCCTTGAGTTTAAACCCCATAGTGTGCTTTAATTTTAAAAAAGAAAGTTTGAAAGGTGAACCCTTAATTAAAGGTGAACTTAGTGCAGTACTAAAGTCGCACTTCTTCTAAAGGTCTGTTTTACTTTTTCGTTTTAGCACAATCACAGTCCATTTTGAGCTGAGTGTTACTAAAACGATTGGCAAAATAATCAGCCACAAATTTAACCATTGCTACAAAACTCAAGATAGCAAAGGCAAAGCCAATTAGTAAAAACACTGCTCCTAAGAAGTACAAGAAACCGGTGGATACTGGAGCATAGTTAAAGCAAACATAAGCAAAAATAAAACTAAGAAAAGTAATAAAGCTAAAAACAATCACCCAGATTAATGTGCGCTTGGTGTTGTGCGGCGTTGTTGGCAACATGTTGCAAATTTCCGCTTTTAACTTCTTAAAAGAACACTTTTTAAACCACTGCTTTTTAGTGGTAGTGCTTTCTTTTTTGTTCATAGGTAATATTTAATGTCTTAGTTTCAATTTTAGTTGATGTTGTCAAACTTACCGCTAAAGCCTAAACACAAACTTACATAGCTTATTTAAATTTAAAGAAGGTTGTTGCTGTAGTTTTGAATAAAAATGCCAACCCGAATGATGGCCTTTTCATACTAAAAGAAGGAGCCTAAAAAAGAAGGATAATTCTGCAAACTGCAAAAGAGTTATAAGAATATCTTTAAACTAACTAGCAGAATTAGCCTTTGGTTTAAGCTTCTTAAAACTGACAAAGTAGAGAAAACTCGCTACCATACACACAAAACCAAAGGCTGCTAACACAAAGGTTAAAACAGAAAGAAAAGTTTTGACCGCAAATGGAAAATGCACAATCTCAAAGGGTTTCAAGTCAACTCCATTAGCATTGACATTAACCTGATACCCGTTAAAAAGTACTAATACACCAAGCGCTAAAGCTGCTATAAACAACAGTAAACCAACTGTTAAAAAAATAATGCTGTTGCATTTATTCCTATTTTTCCCTCTCATAAGCAATGATCTTACCGACACGTTTACTATGGCGCCCACCCTCAAACTGCGCCTGGAGAAATTCATCTACTATTTTAATGTTTTCTTCAGGAACCACAAAGCGGCTAGACAAACACAGCACATTGGCGTCATCATGTTGGCGTGCCAATGCTGCTGTTTTACTGTCTACCACTAAAGCAGCTAAGATACCCTTAGCCTTGTTAGCGGCCATACAAACACCCACACCAGTACCACAAATAAGGATGCCTAAACTATTAGGATTAGCTTGCATTGTTTGCGCTAACAAAAAGGCATAGTCCGGGTAGTCATCAAGTGGATCTAGCTCTGTAGGACCTAAATCAATCACTTGCAACTTTTGTTGTTCTAAGTGTTTTATCACTAAGCTTTTGAGCTCTAAACCAGTGTGGTCAGAAGCAATGTAAATTGGATGGTTCATTTGCATTAGTTTAAATCAAGGTGGTGTTGTTTAGCAGATGATTTAATTTTACCCAACAAACTAATTAAACCACTCAAAGTTTCCGAATAATTCTGCTGTTGGTAAAACTGGGTCAACGTTTCCAAGCTTTCGTTAATTTGGGGGCTTTCTTGGCGGTACTTATTGGCAAAGATAAAGGTATGTTTACACATTGATCTCAGGTTCATTAAACCTACTACATCAACAATCACATGGGTTAGTTGGTTTTGGAAGAACCTCACCTTTTCGTTTAAATAGCTAAAAGCACCCTCATAGTCATTTTTCGCTTGGGTAAAAATATCATCAAACTGGTGCTTAGCTTGACTAATAATCTTTAAAGTCTTTTCGGAAATAATTAACTCTTCATTCGCAATTAAGTTTTCAATTTGGAAAAACTTAAAACGAAAGTCATCTAAGGTATAGACAAAAAAGCGGTAATCATCAAACTTATTAGCTGACAGCGCAAACAGCTCGTTTAACAGATTTTTCAATCCATCCAATAACTTCATCGCTTCACTCATTAAGCGAAACAACTTCTCATAATCAATGAATTCGGTCAGCGCATTTTCCTGGTTGAGTAGTTGGGTTTTTTGTGCAATTAACAGCGAGTGTTCGTTAATCTGGTTAATGATGTTAACGGTATCCAAGTTGTTTTGAAACATTAACTCAATTTCCTTCATTAAAGTCTGTAACAAGGCGTTTTGTTGTTGGTAGTGGTTAATTGACTTTAAGAAGCTATCCACATTAGATACCACTAAGCTAATCGCTTTTTCTTTAACACTAACAGCATTAGATAACACATCAATCTGTTCCTTAGCTTGGTTAATGCGATGTTCACACAACGCTAAGTTAATGTTGCCCAACGCTTGGCTACATTCCGCCAAAGTAGCACCAATATTAGTAATTAATTCCTGAAACTTAGCTACCTCTTGGGAGTTAATTGCTTTGTTTTTCATCGAGCGCAATTGCGTTACCTTTTGACTCAAAATTTGGAAGTGGTAGTTCAAATAGCCTACTTTGACATCAAAGAAGTATTGTCTTGATGCGGTAATAATGGTTTGTGCTAAGTTATTGTGGAGTTTATTCAACGCTTGGTTTTGCTGTTTCACATCCTTATTTTTGATGTGTAGTTCCACATCCTTAAACAATAAGGAAATGCGGTTAATAATGTTGAGGTAAAACAAGGAATCGTACTTATGCACCAAAAAGTCATTAATAAAACTAACTAGTAAACAAAAGGCTTGTCGGTAGAATACTAGGGTATCATAACTACTGTCAAAGTACGCAATTGCGTTTTTAGTCAGCTTAACATAACGCTGTTGCTTGTCATGTAATCCTTGTAAGTTTTTGCGCAACAACTTAATATTACGGTTAAACCAAAAGCAGTTGTAAAGCGCTTTTGGTTCACTAAAAACTAACAGCTGTTCTAAAGCGGAATTGATTTCGCTGTTAAAGCTCTCTTGAAAACTTTTGAGCAATTTCACTGGTTGTTCCAGCGCTTTCTTTTCACTGCGCAACAGTTGAAACGGCACAATTACCGAGTTGAAATTACTGAGTTTTAACTTGTTGTACAGCGCCTTGGTCTGTTCCAAGTACTGCTGGTTTTTATGCACAATAATGAAAATCGTTAAATACCACAAGTAAAACAGAAAAGCTAAGACAGCTAACGATCCCAATAAAGCAGCAATGACAACCATTAGCGTTGCTTATTGGAAAGCTTTAACAGTTCAAACATTAAGTTTTGTTCAATCTTAATGTTGAGCTTTAAGTCCTTCGAGTGGTTCATTTGCCGCTCTAACTCGGCCTTACGCTTCAAAGTCGCTTCCAGATCTATTTCATCCTCAAAGACAAAGTTCTCGGTAAAGATCTTGGCCTCAGTTTTCTTAATGAGCACTAACCCCGCTCCTATGATGGCATTGCGCCGCGTGTTATTGGCAAACAGAATTTTGCAAAAGTGCGATTGTACGGCCGCAATTAAGGGCGCACGGTTATAGTTCAAACCCAAATAACCATTAGTGGTTTTGACTTGCGCACTAATAATGGCTTGATCGAGTTTAATACCACTGGGACTTAAGACTAAAAAGCGCAATGGTTGCATAGTTATTTCTCCTCAACAGTAGCTTTTTTAAGCCGCTTCGCTTTCTCCACTGCTTCCTGAATGGTACCAACAAAGAAGAAGGCCTGTTCGGGGAGATCATCATGCTTACCGTCCAAGATTTCCTTAAACGATTGGATCGTGTCACTTAAAGGGACATACTTCCCAGCAATCCCCGAGAACTTTTCCGCTACAAAGAACGGTTGGGAGAGGAAGTTGCGGATCCGGCGTGCCCGTTCCACAATGATCTTGTCCTCATCCGCTAACTCATCAATTCCCAAGATGGCAATAATGTCTTGTAGTTCCGCAAAGCGCTGTAAGATGTTTTGCACCCCTAACGCTACCTTATAGTGGTTAATCCCGACAATATTAGGATCCAATAAGCGACTGGTAGACTCTAAGGGATTGATCGCTGGAAAAATTCCCAACGCTGCTATGTTCCGGTCTAACACCGTTTTAGCATCCAGGTGGGTAAAGGTGGTAGCCGGAGCGGGGTCAGTCAAGTCATCAGCTGGCACATAGATCGCTTGCACCGAGGTAATCGAACCGGTTTTAGTGGAAGCAATCCGCTCTTGTAATTGCCCCATTTCAGTCGCCAACGTTGGTTGGTAACCCACGGCTGATGGCATCCGACCTAACAGTGCTGATACCTCACTACCAGCTTGGGTAAAACGGAAGATGTTGTCAATGAAGAGCAAGACATCCTGGTTGTCATGGTCACGGAAGTATTCGGCCATAGTAAGCGCCGTTAAAGCGACCCGCATCCGTGCTCCTGGGGGTTCGTTCATCTGACCAAACACTAAAGCAGTTTTGTCAATCACCCCACCCTGGATCATTTCATAGTGGAGATCATTACCTTCCCGGGTGCGTTCCCCCACACCAGCAAACACACTTAAACCAGAGTGTTCCTTAGCAATGTTGTGGATTAACTCTTGCACTAAAACCGTTTTACCAACACCAGCACCACCAAATAAACCAATCTTACCACCACGGGCATAGGGAGTGAGCAAGTCAATCACCTTAATCCCTGTTTCAAAGATTTCTAGCGTATTGGACTGGTCATCAAACGAAGGTGGTTTGCGGTGAATCGGGAGTTGTGGTTTTTTGGGTAACTCCTCCTTCCCGTCAATGGGTTTACCCAAAACATTAAACATCCGGCCCAAGACGTTTTTGCCAACTGGGACTTCAATCGGGTGGTTATAGTTAGTCACCTTTTCGTTGCGTGCTAACCCTTCAGTTGGACCCAAAGCAATGCAGCGCACAATGTCATCGCCTATTAGCTGCGCGGCTTCCAAAAACAGTTCTTCCCCAGCTAACTTAACACTCAAACAATCGTAAATGCGGGGTAACTGACTACTTTCCGTAAAGATGACATCAACTACCGGGCCAATCACTTGGTGGACCTTACCGTATGTAATGTTTTCCTTTTTCATTAACTAATACCTCCTATGATTTCAATAATTTCTTGGGTAATAGAGTTTTGTCTTAACTTATTAAATTGAATACTGTACTTTTCATACAGATCCTTGGCATTCTTAGCAGCGGCATCCATGGCGTTTTGTCTTGATGCTGACTCACACAACTTTGTCTCTACCAACCCACCGTATAACGCAATGTCAAAGAACTGGGGACTAATGAGCTTAATAATTGTCTCCTCATCGGGTTCAAAGTCGAGCTTTTGTTCCATTTTGACCGGGTTGAAGGCCTTAAACATCGCTACATCAAAGGGAAAAACCTGGAAGCTGTTGGCGTGTTGAATTAAGGGGTTTTTAAACTGGGTGTAAATGATGCAAATACGGTCTAATTGGTGTAAATCAAACTGCTCCATAATTTGGTCGGACACCTGTTGACAGTAATCAAAGCTCAAGTCACGATCTTGGATGTCAACATAACCAACGGTGGGGTTATCGGTGTCACCTTTATTTCAAAAAGACTGGTTTTTGCGCCCCAAAAAGAAGATTTTGTCACCGGGTTGGAACTTCGTTTGCAACAACTTGTTCATGTTAGTGTTGTGCTGTCCACAAAGACCCAACGATGAACTCATCATCACCCACAGGGTTTTGGGATTTTCCAGGGGCTTGGGCATCTTCTTTAAGCTGAGAACTACTTGCCCAACGGCCTTATAAAACTCATTGAAGAAGTAGTTAACTTCCTTAAACTGTTTTTTAAAGCGGACAAACTTAGCACGGGACACCATTTTCATTGCGTTAGTAATCTTAATGGTGGACTGCACCGTGGTCATTTTGCGCTTAATTTCCTGGATAAAGGCCATTATTCCAACTCCTCCATCGTTCCGTATTTAGTGATGTCGTAATCTGGTAAAGCACTAACAAAGCGTTTTACTAGTGCCTTAAAAGCAGTGTTAGTTTGTGCTTCCAACGCTTCACTAAAGCTCTTTTGGGTCGCTATTTCCTTGTAAATCGGGTGTTCCTTGTCAAATTCCTCCATAATGCGTTGCTTAAATTTAGCAATGTAATCCAGTGGAATAAACTTAATGAAGGACTTGGCAATCGCAAACAAGAATAACGCTTCATGGGTTTGTGAGTAAGGTTTACCGTTGGGTTGTTTAATCATCTCCATTACCCGCTTACCGCGTTCCAAGATTTGCTTGGTATTTTCGTCCAAGTCACTACCAAACTGACTAAAGCTGTCTAATTCACTGTACTGCGCTAGCTCTAACTTTAAGCTCCCGGTTTGTTGTTTAATTGCTTTAATTTGCGCTGCACTACCAACCCGCGACACCGATAACCCCACGTGGATGGCAGGGCGTTGACCGGAGTTAAACAGGTTTGACACCATGAACAGTTGACCATCGGTAATCGAAATGACGTTGGTCGGAATGTAAGCGGAAATGTCCCCAGCTTGCGTTTCAATAATAGGTAAAGCGGTAATCGAGCCACCACCATGCTCCTCGTTTAAACGGCAAGCACGTTCCAGTAAACGCGAGTGCAAGTAGAACACATCCCCAGGAAAGGCTTCCCGTCCTGGTGGTCGGCGTAACAGTAAGGATAAGGTACGGTAAGCAATCGCGTGCTTGGACAAATCATCAAACACAATTAGAACATCCTTACCCTGTTTGAGCCAGTACTCAGCAATGGTAATGCCAGTAAAGGGCGTGAGGTAGATCATCGAAGCGGGATTACTGGCTGTAGCACACACCACCGTGGTGTACTTCATCGAATCAGTTACCTCTAACTGGTGGACAATTTGCGCTACCGAGGAATTCTTTTGTCCCATCGCTACATAGACACAGTACACGTCCTTACCACGTTGGTTAATAATGGTGTCAATTGCAATTGAGGTCTTACCAGTTTGGCGGTCCCCCACAATTAACTCCCGTTGTCCCTTACCAATCGGAAACAACGCATCAATCGTCAAGATTCCAGTTTCCAGTGGTTGGTTAACGGTTTTGCGGGTCATTACCCCAGGGGCAATCTTCTCCACTTGGTCAAACTCTTCAGCCTTAATCGGACCACGACCATCCACCGGTTCTCCCAGGGCATTGACAATCCGGCCCAACATTACATCACCCACGGGAGTTTGCATCACGTTGTGGGTCCGTTTAGCCGTACTACCTTCACGGATTTTAGAGTAATCACCAAACAACGCCACCCCAACCGTGTTTTGCTCTAGGTTAAGCGCAATCCCCTGCACGTTGTTTTCAAACTCAATTAACTCGTTTAAGAGTGCGTTTTCCAAACCACTGACCTTAGCAATGCCATCAGCGACACTAATAACCTTGCCAATTTCACTGTTAAAGATCTGTTTGGAATACTTCTTAATCTCATTTTTAATGAGTGCTACATATTCATTTAATTTGTCTGCCATAATTAATTCCCTTTAAACTGTTCCATTAAACGTGATAGTTGATCACGGATATTCTTTTCAAATAACTGGGCGCTCGATTCAATTCGGATACCCGAAATCAAGCTCTTGTCAATTTCGGTTTTGTAAACAATCTTGGAATTGAGCTTCTTTTCCATTGCCGCTACGATTCGCTTGAGTTGGGGCGCACTCAGTTCAAAAGCGGTAATGATCCTAATGAACTGGACGTGTTTTTCATTCTCAACACAATCAAAGAAGTACTTTAAGGCTTGCTCGACTAGGTTAAAGTAGCCCTTGAGCACAATCGTTTTTAAAAAGTCAACAAAGAGGTCACAAAAGTGGTTTTTAAACAGCTTATCTATTAGCTTAAGCTTTTCCTTTTTGGTTAAAGTATAAGTATTGAGTAGAGCAGCTAGTGATCCATCTTTAAAGTTACGCGCTAGTTTTAAAAAGTCATGGCTCTGGTCATAGACCTTCTTAACCTTCTTTTCCTCTTCACTAAGTTGGAAGAGTGCCATTCCAAAAGCCTGCGCGTTAATCATGGTCGTCCTCTGCTTCTAGTTCGCGGATAAACTCTTCAATAAAGGCCTTGTCGGCTTTGACATCCACCTTTTTGATAATCAATTCCTTAGCAGCTAACATGGCTAGCTCGACACTTTCCTTTAAGGATTGTTCTTGGATCAAGCGCTTTTCCTTTTCAATTTCCTGACGCGCTTGGAAGATCATTAAGTTAGCTTGCCGGTTAGCCTTCTTTTCGATTTCACTTTTAAGTTGCAACGCCTCATAGTTGGCCTGGTCTACAATTTCCTTAGCAACTACCAGTGAGTTTTCATGGCGTTGGTTGGCTTGTTGGAGTAATGCTTGAGCTTGTTGTTCTAAGCTGTTGGCCTGGTTGACTTGCTCTTCCAAAAGCGCTTTGCGTTGGTTTAAGAACTTTTGTGTGGGTTTTCAAAAGAGGAACAACAACAAGAACAACAGGATCACAAAGGCCAACAAGTGGGCTAAAAAGACCCAGAGGTTCGGAAAAAGTTCGTTGATGACTGATTCACTTTTAATTTCCTTAACGTTTTCGGTACAGGAAACTAAAAACAAGGCAAACAGGGCAAAACTAAGCGCAACTAAGGTCGTTTTAAATACAAAAGTTGCTCGTAGTTTCATGATTAAGCGCCCGATACAAAGATAAGAATGAAGGCTATTAACAAGGAATAGATGGAACTCGATTCACTAATAGCAGAGCCAATAAAGATTAATTTAAAGACCTGCTTTTCCACTTCGGGGTTCCGCGCTACGGCTTCAACCGCTTTCCCAAAGATGTAACCTTGACCAAGACCCACGGTCGCACCACCGACCATGGTAATCCCAGCCCCAATGTAAGCCCCTAAACGTTCGGTACTTTTATTGGCAACATTGGTATTAGCTGTTGTTGTTTCGTGTAAGATGCGGCCAACTGTGGCGAGAATTTCATTAACGTGTTCCATATCTAATTAATTATTTCTTTTGCTACCTCCAAATTTTGCAAACGCTGCGCTGCTTCTTGACTTTCTTCCAAGCCAATGTTACGCATTTTTGCCCAATAATTATAAGTCAACATCACAAAGACATATCCCTGCAGTGTACCCACTACTACATCAAAATATACATGTAGGGCTGGGGTGAGAATTCCTGCAAAAATGGCACCTAAACTAAGCGCTAGTGGCTTATTGCTTAAAGTCGAAAAAGCAAAGAACCAGAAGTTATAAAAGAGCGCTAAAATCAAGGTTCCCCCAAGGATATTGCCCCACAAACGAAGCGAAATCGAGAACAGTGGCGCAAACTCACCCAAGAAGCTCAACGGATTGGGAATTAAAGTGGAGTACTTCTTGCCCTTAATCGTAATGTTGAAGGTAAAGCTTTTAAAGAAGGATCACCGTTGGTACCTAATCCCCATAATGACAATTCCCAAAAAGGTGGCTAGCCCTAAAGAAAAGGTAAAGGTCAGTGATGAAGTTGGTGGGGAAATGCCACCTAATAATCCAATCAAGTTACTGGAAACCAGGTACAACAGCAACATTAAAAAGTAAGGGGTAAACTTCTTGTTTTCCTCACCAATTAAATCCGCTGTAGTATCTTGGGCTCAAACAAAGAGCATTTGCATTAATAACACAAAGCTCGAATGGTTGCGTAACGGATCCGCTTTTCTTAACTTGATTCAGTAAACCAAGAATAAGGTGAGCAACACAATAAAGACCACAAAGATGCCAAAAACTTGATCAGTGGGTAGCAATGGTTTTCATCCCGCAACTGGGGAAATGTCAAAGATACCTTCGTGTGGTACGAGGTTAACTCCATTAGGTTCTTTTAAAACTATCGCCCGTGGTGACATGCTTCTTATTGAGCTTGGAAATCAGGTTTTTGCAATGATATTCCAAAGGTAGCCATGCAAATGATATCACTATTTTTAGCAATGGGAAAAATAACGTTGAAATTATTGTGGCAATCACATGAAAATATTGCGGTCTTATAAAAAAGGCGAGCAATACGGGAATCAGGTACAGCAAATACCGTGCCACAAAAAAGGAACCAAAGAAAACCGTAACTCGTTGTTTTGATAAATGCTTAAACTTTTCAAAAAAGTCCCCTAGTTTTAATAAACCAACCACGCTTAACGCTACAAACGGAAAGGGTAGTAACCAACCGTATAAAACTTTTAAGTGTTGGGTAGTTAAAGCTCAAACTAAAATACCCACTAAAACCACTAACCAAAGCACCAAGATAATGAGGGCAATTGCTTTAGTGAGTTTTTTATTGGTCCAGTTGTACAAGGCCGTTAAGCTTTTTGCGGTTTAATGTTTTTAAAGGTGTTTCAACCTAAGTACTTAGCATTATTGCCCAATTCCAGTTCAATTTGCAATAAGCGATTGTATTTGGCAATTCGTTCGGAGCGGGACATCGAACCAGTTTTAATTTGACCAGTGCAAGCCGCTACTGCTAGATCAGCAATTGTCGTGTCTTCGGTTTCCCCACTACGGTGGGAAATTACCTGACTCCAGTTAGCGTCCTTGGCAATGTTAATCGCTTTAATTGTTTCTGTAAGGGTACCAATTTGGTTGAGTTTAATTAAGATCGAGTTGGTAGCTTTGTGAGCAACACCTTTTTCAGCAATTGCTGGATTGGTGCAGTACAGATCATCACCAACTATTTGAATGTGTTGACCCAGTGTTTTAGTTAATGTTTCCATACCTTCCCAGTCGTGTTCGCTAAGACCATCTTCTATGGAAATAATAGGGTACTGCTCAGTAAGCTTCTTGAGGTAAGCGATCATTTGGGCAGTAGTTAAACTCCATTCCTTCTCGTCAAGAATTTTGGCCTTAATTCCTTTTTTGAAGACATAGCGCTTGGTGGTGTCATCGTAAAACTCACTCGCTGCTACATCCAGTGCAATCGCAATGTCACTACCTGGTTGGTACCCAGCTGCCTTAATTGCTTCGACCATTAAATCAAGCGCTTCTTCCGCTAACTTTAAGTTAGGCGCAAAACCACCTTCATCACCCTTATTGGTGTTTAAACCCCGTTGCTTTAACAGCTTTTGTAAAGCATGGAAGGTTTCACTCGCCATCTTCAATGCTTGGTGCATCGAGTTAGCACCCAATGGCATAATCATGAACTCCTGGAAGTCAATGTTATTGTCAGCATGTGCTCCACCGTTAATGACGTTTAGCATTGGTACGGTGAGGATAAACTCGGTTTTATTTAGACCCATGACCTGGTTAGCAAGGTACTTAAACAAGGAAGTCTTTTGGGCGCTAGCAGCTGCTTTAGCCACAGCTAGAGAAACTGCCAAAATGGCATTAGCACCTAACTTAGCCTTATTGGGGGTACCATCCAGTTGGATCATTAAAGCATCAATGGCTGCTTGATCAGTCGCATTTAAACCAATTAGTTTAGGGGCAATAGTTTGGTTAACGTTTTGCACAGCTTGGCTCACCCCTTTGCCAAAGTAAGCTTTGGGATCACCATCGCGTAGTTCAATCGCTTCTTTTTCCCCAGTAGAAGCCCCAGATGGAACCATCGCTTCTCCAGTATGACCACTAGCTAACTTCACAACACAAGCAACAGTGGGAAAACCCCGTGAATCAAAAACTTGGTAAGCAAAAAGATCAGCAATCTTGAAAAGATCGGTTCCAGTTTGTGCACTCATATGACGCTTCTAACTAATATTTACTTAAATTATATTGACCAATAAATTTAGGAAATGAAACAAATCTATTTTGGTGGTGGCTGTTTTTGGGGTACCCAAAAGTATTTTGATCTGATTAAGGGTGTCCAAAAGACCAGTGTGGGCTACCTCAATTCCAACATGAAAAATCCCACTTACGAACAAGTATGTAGTGGGCAGACCAACGCAGTGGAAGCAGTGTTCGTTGAATATGATGAAAACAAGGTAAGCTTAAACGAACTTATTGATGCTTTCTTTAAGGTCATTGATCCCACCATTAGAAACCGTCAGGGTAATGATATTGGTACCCAGTACCGTACCGGAGTTTATTGGGTAGATCCCCAAGACGAGCAGTTAATTACGCAAAAGTTTAGGGAACTACAGGCCAATTATCCAAAACCAATTGTGACGGAAAACCGTGCAATGGAAAACTATTTTTTAGCAGAAGAGTACCACCAGGATTACTTAAAGAAAAATCCTCATGGTTACTGTCACATTAAGTTTGACTAGTTAAAGACTTCTGGGTTTTTCACAAACAGGAATGCCTTAATGATGTTGTAGCAATAGTCATTAATCCGTTCAATGTGTTTCACCGACAGAATTAAGTCAATTAAGAAGATGCGATCACTCAACTTCTCGTCTTTAACCTTGTCATAGTATTCAGTTAACACGACGCGGTACTGCTGTTCAAACTCTGTCAGAATTTTAGTGACATTCTCAAACTGAATAGTAAGCGGTTTTTCCTTATCTTGTAAACTGGCAAACGTTTGCTTTAAGTTGTTCAACACACTTTTGTGCAAATTAACTAACTTACTAAAGATGCTGACATCCAAGTGCTGGTAGTGCTTGACAAACTTAGTGAGGTTATTGGCATAATCACAAATGCGTTCCAAATCACGACTACACATAATGATCGTAATTGTTAAGCGTAAGTGGTTAGTTAACGGGGAGTTTTTTGAAATCGCTCAAATGGATTCGTTAATTAGTTTAAACTCCGATTTATTGGAAAAGTCTTCCATTTCGTAAATTGCTTGAATCAACTTTTCGCGCTTAGTTACATCCGCTTCACATAACAGTTTATTGAGTGTTTCATGCATCTTAATGACGTGCTGAAAGTAATCAAAAAAGAGTCCTAACAGCTTTTTTTCTGAACGCTTTAAAATTTGGTAGTTAATGCTTTCCATCGTTAATTCCTCCCACTAATGTAACGGTTAGTTTCCTTTTGCTTTGGTCTCGTAAAGATTTGCTTGGTAGTACCTTGTTCCACTACCCTTCCGTTAGCAAAGAAAATCGTTTCATCAGTGATTCTAATAGTTTGTGCCATTGAGTGAGTCACAATAATAATCGTGTATTTTTCCTTTAATTGCTGGATTAACAGCTCAATCGAGTTAGTGGCAATTGAGTCCAACGCACTAGTTGGTTCATCCATTAATAAGACATCGGGTTGTAGAGCAATGGCTCTGGCAATACACAACCGTTGTTGTTGACCACCCGAAAGGGTATTGGCATTACGGTGTAAGTTATCTTTGACCTCGTCCCATAAGGCGGTTGACTTTAAGGCCTGTTCCACAATTTCATGAATCGCTTGCTTGTTATGAATGCCATGGGCTCTCAGTCCATAGGCAATGTTTTCAAAGATGGAAAAGTTAAAAGGGGTGAGTTGCTGGAACACCATACCAACCCGTGTGCGCAAGGTTAAATCATTAATAATTCCGGAATTAATGTTTTTACCCAAAAAGAAAATCTTGCCATTTCAGCGCACATTTTCATTGAGGTCATTTAATTTATTTAAACAACGGATAAAGGTGGACTTACCACAACCGGATTTACCAATTAAAGCGGTAATCTTATTGCGCTTAATTTTGAGGTTAATGTCAAATAAGACCTGCTTGTCCTTGTTGTACCAGAAGTTAAGGTTTTCAATTTCAAAGACATTCTTCTTGTCAAAGTCTTGTTTGACAACATCGTCCTTGGTATTCTTAATTTTGATGAGGCGTTGCAACACATCAATTTCACTCTGCAAGTTAGCAATTTCCTCAGCTTTGGTAGTGGGATCTAACTTAGCAACTTTTTGCTGGTATTGCTTAATTTGTTTTTCATATAAAGCACGGTACTGCTTCACCTTTTCGCGCTTTTGGATGAAGTTGTGTCAAATTGTTTTTAGGCCTTTTTTCATAATTGCAATTTGCTTTTAATTTGTAATCACTTTTGTTTATTAAACGAAAACAGGTAAGGAATTAAGTAAGAGCTTAAAAAGATTAAGAACATCAGGAAGACAATTGATACCAAAGCGGTTTCCAGCATTACATCAACCGCCGTACTGTTAGTGGAAAATAACTGACCATAAATTCTTGTGGTTAAGGTTTGACCCGGTAGACTTAAATCAAACAAGTTGCTGGATGCTAACCCAGCGGTAATAAAGAAAGGAGCTGTTTCCGCAATAATTCTGTTAATCGTTAAAATAAGCGCAATAATTAAACCCTTCAACGCCCCTGGTAACACAATTTTCCAAATTACTTCCCTTTTACTTACACCTAAGGCATAAGCACTTACCCGCAAGTCCCAACTGACATTATTGAGTGCTTCCTGACATGTGCGAATTAAAAAGGGTAAGACCACTACACTAATAGTAAGAATACCCGCCATTAAACTAGTACCATTCGCACCACCCGCACTTAATTGCAGTGTTCGCAAAAAGAAGGATAGACCAAACAATCCGTAAATAATCGAGGGCATCGAGCTGAGCGAATCAATTACAAAACTAAAGGTATTTTTTGCAATCCGCGACTTCGTGTATTCGTTTAATCAAATGGCAATTAACAAAGCGATTGGAAAGGTAATACCAATGGCAACCAGAATAATCACCAAGGTATTGACTAAAGCTCGCCCTGTAGTATCTACACCAAACGAAAAGACAGTGGAACCACTGCGCTGAATCGCTTGACTACCATTAATAAAAACAAACAAGAGAATCCCTAACACAAAACCAAACGCTAAGACGGCACAAAAAATTTCCTGCAGGATTTTTCACGATGTGTAAACCTTATTTAAGTGGTTAGTCTGCATCCTTTCCTTGAAGAAGGTAGCGCGCTGCTCAACATTAGCAGCTGTAATCTTTTTAACCTGTTGTCTTCTAGCAGTTAAATCAGTAAAAAGAGCGCCAATGTTATTAGGAATAAACCACACTACTTGATAAATAAAGTTACTAATCTTCTTTAGGAAAGGATAGCGTTCCAGCGTTTTTGGCCGGGTCGATCAAATGGCAAAAAAGTTCAAGAAGGAAATTAAGACAAAGATAATAATGCCAAAAGTGTATAAGACCCCACTAACCTGTTCATCGCCGTTTTCCGAAAAGATAAAGGTGGAGATAACAGAACCCAAAGTTTTGAGATCTGAAGTAAAGAAACGATCATTAGCAATCACTTCCTGGTAGTTAACACTCTGCAAAATAAAGTTCACAGCCATCGTTTCGCTAATGGCACGACCAAACGCTAATACCAAGATTACTACTAGTTGGGCTTTGATTTCCTTTTTAATTACCTTATAAATCACACTGGTTTTGTTTTCACCAAGTGAAATGGCAACCGTCATTAAGCTAGATTCCACGTGCAGTAAGGCATTGGTGGTTAAGGAAATCACAATCGGAATGATCATGAAGGACAGCATCACAATCACGTTAAGCAGTGATAAGGGTGGCAAGTGCAACACGTCACGGAAGATACTGCTGAGAATTTGGGTAGCAAACAATCCAAAGATCACTGAAGGGATCCCGGACAGAATGTCAATTACTAAAAGTAGTTTTTTGCGAATCCGGGGTTTGCAACGATAAACCAAGAAAATCGAAGTGCGAACACCGATATAGCTCGCAATAATTAACGAGCCAATCGAAACCACAAAACTAACTAACAGCGGAAACCAGATGCTCGCTTTTTTAGCACCCAAGTTAAATTCCAAACCCAGTAAGGACTTGGTAAAGTCAGGACCAGTTTTAGCTGCTTCAATTAAGAGGAAAATAAAGAAGCTAATGAAGAGCAGTAAAAAACAAACAGCCACTGTTTGCGATAAGTACCGCAGCCAGTTGTCTTTTTTTAAACGGCTTTTAATTTTCTGTTTCACTAGAGTGCACCGAAGGTTTGGTTGTTCAGACTGTAATCATCAACCCAGAAGTTGGCAATGTTGCTCATTTCTGCATTACCTGTTTTAAACATCTTTTTCTTTTCGTCAGCCGTTAACTCTAACACACCAAAGTCATCGTATATTTTCTTAATTTCACTGTTGTTGCTAAATAACAATCAGTTAAAAAAGCGTTGGATACTTGGCAGTGTTTTTTGTTTTGGTAACAGGGAAACCACTGAGTTCAACGGGCGTACCCATTTGTAAGTGTTGCTAGAAACTGCTTGACTGGAGGGGGTAACTGCGTTATTATCATATTTGACTTTTAAAACCTCAAAACCTTCACTCTTAATTAGGTTCATGTTGTTATTCACAAACCCTAAACTGAGGTACACCATGCCGTATAAATTGGGTTGTCGCAAAGTTGTGACAAAGGTATTAAAGGCCTCAATGTTGGTTTCACTAGTTGGTCGCGCATTGGGGCCATAGTTGCGTTGACCTTCCAAAATTTCTTTGGAATCTTTATCTAAAGTTTTTGTCTGTGTAAGACCCGAAAACTTCAAGAAAGCTTCAGCGGTACCAGAAGCAAAAGCGCCCCCAGTACGGGGGAAGCCTATCAAATTATGATTACTGTTTTTAGTGGTTTGTCCGTTTTCGACAAACTTATTAATGTCAACGCTTTTGCTACCAGCGAACAGATCGTAAAGATCGTTGAGGTTGTCTTTAGTGAGAACTAGTTTGCCCTTAAACTCTGATGGTGCTTTATAAATAACAGCAATGGCATCTTTGCCAAGTGTAATTGTTTTGAGCTTTTTGTCCATCCACAACTGCTTGTTTTCCTCAGCATAGCTCTTGGTGTTTTTCGAGACATTACCAATGTCAGCTAGTCCCTTGGTGATTGCTTTCACCCCAGCACTAGAGCCACCCGCTTGGACACTAATTTCTACAAGGTTATCCTTGTCATTGTGGTTCAAGACATAGTGCGAACTGAGTTTGCTTAACAACGGTTGTACCGAAGAAGAACCAACAGCACTAATTAAATTAATATTGGCACAACCGCTAAAAATAACAGCGGTTAGTGATAGTAATGCTAGTCTAGAAAGTCTTGCAACTCTTAGCACTTTTCTAATTGTGTGCGTTAATTTTAGAAAAACTGGCATTTTCGGTACTTTTTAACCAACTTTTAATTAAGCAAGTTGCATTTCTTAGTTGTGCTTTAAGTAGTTAAAAACAACGATTTTCTGGCGTTTATTCTTTGCCAATTGTTTCCTTTGCTAATTTCTTTTTACGAGAGGCTGCTTTTCTTTTAATCATTCAACCAATGGCCGTGGCACCTAAAGCTAACACTCCAGCAATCGCTGATAAAGATCAGGTCAACATCTGGTTAGTTTTAGCACTAGTTTAATCAGTGTCATCTTTATTGTCCAACTTTTCCAGTGCAAAGGCTTTGTTTTGGGTGTGGAAATCAAAGACTGGTTTTTGTCTAAACTGGAAGTTGTTTTCCTTTAAGAATCTAGCTAGTTGTTGTGCTAGCTTAGTTTGACTGTCATTTTTAATGTTGAACCAAACTGACTTTTCTAGACCATCTTTATTAATGAGATATAGCATTTGCTTATTTTGTGATAGCTTTAAGTCATAGTTAATTGCAAAAAGTCTTTGACTACCCAACATTGCTTGAATGGAGAGCTGACTAGTATAGCGATCAACATGGAACTCTAAGTTGAACTTTTGGTTGTTTTTTAAAACGGTTAAATAAGCCATTTTATTTACCGGATTCAACCTAGCATTCTTCGTTTCCACATGGAGGTTCATTTGACTCAAAACAATGTTGAGTTTTTCCAATAACACTTGTTCATCTTGCAAACTAAAGTCTGATTGAAACTCAAACTCATAGTCATCTATTAATTGGATGTTGGATGTTTCTATATCTAATTTGCCAATTTCGAATTCATCAAACATAAAGCTCACTGTTTGGGTTTCCAAGTGGGGTTTAAACCACAACTTTTGTTTGTCAAGCAACAGCTTGCGCGTATTAATTTTGAACAAGTAAGTGCGTGTGTTGGGCTTTATTTGATTTTGTTTAACAAAGTACATTTGCCCTTTTATAATTTCGGGATCGATCGTGGAACCTAACTTAAACAGTTCGTTGCTGTTGTGGATGTCGGCTAACACATAGAAGAAACCTTTGTTTTCGTTCATGTTTCCTAAATCAAGTTTGTATTGTCGGGGATCTTCCACAAAGGTTAAGCTGTTAGCATCTACATCTAACACGGTTTTATCAACACCAGCAAAACTGTTTTTAATGACAAAAACAATTTGTTGCTTTTCAAAATCATTGAGTTTTTCCATGCGTTGCTTTAACTCACCTACACTAAAGCTTTTAATCGCTTTGTGTAACGGAACACTAAAGTCCTTTTATTTAAATTGCTGGTTGCTAAAGTTACTGCGATATTTAAATTTGTACTGGTAACCTTCTGGAATTTTGTTGATCGTTGGTAGGTTTTTCACTGCACCAAAAAGTTGATCAACTACTTGGAAGTGCAAGTTTTCTGATTCATAACCGTCAACAATAAAATCCAAACTTAAAAAGTTAATGGTGTATTCGTTGGAAGCGTGTTGTTGTGGTGTCAAGTATTTTTCTCAAGTTTTGATATCAAACACATTGACATTGTTTTTAAGCAACGTAATTTCTTGCAAGGTATCACCAATTTTGACTTTAGCTAAAAGTTTGGCTCGTGCAAAAATAGTTTGCAACATCTCTGGTAAGTTCCTAATGAAGATAAGGTGTTCTTTATTGGTAGGCTCCATCCCTAATAAGTCATAGAGGTACTGCAAGCTAGCAAAATTAACGTTGCTGTCCTTGGACTTTAAAGAGTCAAACTTTTGTAGCGCTGTTGCTTTATCAGTAGCTAAATCAATCTCTTCGTTCAACTTACTAATTTGGGCAATCGCTTGAGTAAACTCCTTTTTGAACATTATTTGTAATTGATCTTTTTGAGATAGATCTTTTTCATTAAATGTTTTGGACAGAGTATCCACTACCGAATTAGGGTCAAATTGTAATTGTGAAAGTCACAACCTAGAAAGTTGACTAAAACTAATAGGTGGTTTTAGATCTGCATTATGTTGTTCCACATAAAAGTCAACACCAAAGCGCTGAGCGTTTACTGGGATAATTGGGGTGTATTTGAAAATGTCCCACTTATTGCTAAACAAACTTTGTTCCTCATCATCAGTTAATGGCTTTACTACAACGTTTTTACCCATATTCAAGATTGATTGGGTTACTTGGCGGTTTCTAGTAAAGGCTTCCATGATCTTACGATCTTTAATTACCTTATCTTTAAAACCATTGGAACTACTTCAATATGCATTTGCTTGCTTGTTATCAATGGAAAATACTCAAGTTTCTTTTGGTAATACTGGCTCATTCCACTGAACATTATCTTGTTCAAATACTCTAGGTGTTTTAATTAACTCCACCTCTCAAAAAACACGATAAATGTAAATGGGAGTTGATTCCAACCACCAGTTATTTCAAAGAGTGGCAATGAAAATATTGTTTGGTCTATCTTCAAAGAAACCATCATTTTGTGTTATTGCATAGGCATTAAAAATAATAAAGTAATTGCCATTTTTTCCTTGTTTAATTCACTTAGCATTTTGAACAATGTCATTTATGTTAGGTGACTTAACTGATAAGGAACGGTTCATAAAACCGAAAAAACATTAATCCATTTAAAGTCTTTATTTCTATTCCATACAGGACTAAATAGCCTTTGGGTTTCTAAGTTATAAGGAATAGATCATCCTGCGTTTTTAGCTTCTTCGGCAGTTCGACCAGCGCTTAATGTCACATATTTATCATGCTGTGGATACCACGGGTCATTAATGCGAGATGGTCCAAAACCAGACTTACTATCATTGTGAAAGTGGAAATACTGAAGGTCGTGTATATCTGATTTAAAGGAGTTTCTAACTCCGGGATAGCTAAAAGTTTTGTTCCATGCCAAACTGCTAGTATAGCTACTTTCAATGCCTAAGTTAACATCTTTGATGTAATAGATATTGTTATACCAATTAAAGTTATTTTCTCTAAGCAATTGGTTATACCGGTCATAATAGCCATCCGCTAGCTGAGTTCAAAGGGTGGAATAGTTTCAACCAAAAGTAGTTTTGTTATTTGGTTTATAAATTAAAGGATTTTCGAAATTAAAGTCTTTACGGAAATAACTATCCTTTAATTTGATCCCAACAGTAAACCCTTGATTGTGGTGTGCTGAGTTAGAAATATACAAACTACTATCAACAAGATTTTTATCCAACGACGCGTTCGCTAAAAGAGTTGGGAACGCAACAAAACCTAATAAAGGTAAATAATGATATGAGCGCATAATTAATTCACTTTTTCATCTATTAAATTGCTCAAAAAGGGAATTGACGCCAATTTTTTAAAAGTTTTTATTTATCCGTTTTGACAACTGCTTTTTGTTATTTTTTAAAAGTTTTTATTTATCCGTTTTGACAACTGCTTTTTGTTTGTGCGATTTCAAACTCTTTTTGCGCTTGTAAAAGTAAAAACTAAAAGCAACTACCACTAAAAACAGACTACTAGCAGCAATTACTAAGTAACCAATAAAATTGGTGTTTTTATTTTCACCATTATTTGGTTGAGCCTGTTTGGCGATTTTAAAAGTTGCGGTATTATCCTGCAGTTTAAGCGGAATCTTTTCTTTTAAGTACATCTGCTTACTTTGAAGAAATAACTGCAAGGTATCGAGCAATTTAGCTTGGTATCGTTGATCAGCAACAGACCAGATATACATGCCTAAATGCTTGTTGCGGTTAACAAGTTGTAGAGTGTTTGAATTTTTAATCACTAGTTCATAATCCAACTTAAATAATGGTTGTTCGTCAACCTTAACAATTACATTAAGTTCACTAGCTTTTGGATTAAATTCAACATCAAAATCAAACGATTGATCTATTGACTTTGTAGTGAGTCTTATTTGTTTGGGAAAAGTTTCTATGTTAGTAGCTTGTTCATCAACCATTAAGTTCATGCCAACTAATGCAAGGTTCAGTCTTCTTATTAAAAGTTGCTTATCAGCTAACGTTAAGGTAGAAACCAAATCTACTTGGTAATCGTTAAAGAAAGCAACGTTAGGCAATTTGGGATCAAATTGATCTAATTCAATCCCGTCTACAATTACCTTAACTTTGTCATTGTTAGCTTGAAATCAAACGTCATTGGCATTCAAAGCAATTTTGTGTGTTCTCATTTGAAACAAGTAAGTACGAATTGTTGGAATCTTTGTTTCTTTTTTGAGAAAGTACATCTGGCCTTTAATAATTTCCCGATTGGAATCTGTGCCTCATTTAAAGAAATTATTGCTGTTAGTAATTTCTGCTGCAATATAGAAGATGCCCGTTTTAGGATTTGTCGGAATTAAACCAGGAATTAGTTCTTCAGGATTTTTAACTAATTTGATTGAATTTAAGGCAACAGTTAATGCCGTATCATCCTGAAATGAAAAAACCATTTTTAATTACAAAAGCTATTTGGTTTTTCTGCTCTTCAGTCAATTCTTTATCTTGGATGTCTAAAACGCTAAACTCCTTGCCGTTATTAACGACGGGAATGCTAAAACGATATTTATTGAAATACTGTTCGTTGTAATTTGAGCGAAACCTTAAACGGTATTGAATGCCCTGGTTTGTTTTTTGAATGGTTGGTTTATTTTTAGCAGATTCAAATAAAGAAAACACAGGTAAATTCTTCGCTGTTCAACTAGGATATTGATCTGATATGAAATCAAAAGAGAGAAATTCAACTTCAAATAAATTAATGCTAATTTGATCTAAATTTAAGAATTGTTCAAACTGGCCTAGATTAAAAACGTTATTGTTGTTTTTAACTAAAGTAATCTTTCTTAATTGCCCACCCATCCTAACTTGAAAGTTAATGGATGCAGACTCAAAAATTGTTTTTAAAAGCCTTGGTAAATCACGAACAAGACTTAATTCTTGAGGATCCCTAAAATCAAAATTTAGCAGTTTATAAAGGTAGCTTAGGCGTTCTCATTGCTGTTTTCGTTCAACATTTTTAAGCGTTAAGAAATTTTGTAAAACTGTATTTTTATCAATTGCTTGATCAACTAACTGATTAAGCTCTGTAATTTCTTGAATCGCTAAGGTAAATTCCCTTTTAAAACGTAATTTCAAAAGAGCTTTTTGGCCAAGCGTTCGGTTATTAAAGGTGATAGAAGTATTATCAACTATTGAATCAGGGTCAAACTCTAATTGAACTAAAATAATTCGCGATAGCTGATTGAACTGAATAGGTGATTGATTTTCGCTCTCTACTAAATTTTTTGTTAAGTAAAACGAGCTGTCAATTACATGTGAATTGATAGTTGCGCTAGAGAGCAACAGTGGGGTTAATGAAAAACCCATTAATTTTCAATATTTCAAAAAAGGCATGGCAATCTTTACATTCAATAAATTGAACTAAAGTTTTTAACTTGCCAATTTTTAGAAAATTAGTGCTTTAAAGCTTTTTAAATTACTAAGCTAATTGTTTGGAAAAGTGAGCAATCTTGCTAAAGATTAAATGGTAGTAATAATCCAATTGCTTTTTGCGCATTTCGATTTCAGCAGGAATACCTTCTACTAAATCATTGCAAAGTTTTTCAAAGGCAAAGAGAATATCAGCAATCTTTTCTTGAATTTCTAGAGGTGGAAAAGAGAGTTCAATTTCAGCCATTACTTTTTGGCTTAGTTTTGGTCTTGAAGCTAGATTATGAACGAATTTTGGTGCTTCTATCTTTAATAAAAAGGATAGGAATTTGGTGCAGATTTTTTTATTTTTAACTTTTAAAACACCGCAATCTTGTGATGCATTAAACTTGCCATTACGATAAAAAACTACTCCAGCATAACCATTTGTAGTTCAAGTGATGTATTCACCATCAAAGTCGCAATCTTTAATGCGTCCTAACTCACCATCATTTGTGGTAGCTGCGGAATAAACTGGATTTTCTCCAGGATTATTCCTAATATACGCTTTAGTGATCGCTCGACCTCTTCTAATTTCACAAATATCCTTTACTTGAAAAGTTTCGAAAGGTATATTTGCTCCGTAAATTTTTCGGATATTTTCTTGGTTGAGCAGATAATCGCGATAGAAGGCATACTGCTTCTTTCGCTCTCTTAGCTCGGCGCTTAGCTCGGAAAAGTATCTAAGATAGTAGCAATTTTCTCTTGAATTTGCAAAGGTGGGAAATCTAGCTCAATTTCTGCTAAAATTCCTTGCATTAATTTAGGAATAGGGCAAGCATAATTAACAAATTTAGGTGTTTCTAATCTAAGTGCATAAGCTAAAAACTTAGTATTAATTTCATCACTTTTTACCTTAAGAATTCCACAAACATTGGTTGCGTTAAATTGACCGTTACGATAAAAAACACTACCAGCTTGGGCTCCATCAGTTGTTCAAGTGACGTACTCTCCATTAAAATCACAATCCTTTATCTTACCGATTTCACCGTTATTAACTGTTGCTGATGAATAAACAGGGAATTCTCCTGGATTGTTTTTGATATATAGTTTGCTGATAACTCTACCGCGTTTAATATCGCAAATATCTTTGATTTTGAAACGTTTAATCAACACAGTAAAACTTAATCTCCTTTAAGTTTAAAGATTAAGTGGGGAACTGGACAGCTCAAAGTTTATTGAAAAAAACTAACGCTTGGTAAATTGTGGAGCACGGCGGGCACCGTATAAACCGAACTTCTTCCGTTCTTTAACCCGCTTGTCACGGGTAGTTAGTTTCTTGGACTTGAGAATCTTTTTCAATTCTGGATTGAACTGTAAAAGCGCGCGAACAATTCCTAAACGAATTGCACCAGCTTGTCCGGTAAAACCACCACCTTTAACTACCACATTGATATCAAAGTTCTTTTTAAGATCAGTGAGATCTAAAGGTTGTTCCATGTCTTGGATCACTATTTTATTGGGAAAGTATTCACTAGGATCACGACGGTTAACAGTAATCTTACCCTTGTCTTGGGTAGGCGTTAGATAAACTTTAGCACTTGAAGACTTACGGCGTCCAAGTCCATAATAAGATTGTTTTTCCATTTATATTAGCTTCAATTTAAAGTGGTAGGTTTTTGCGCTTCCATGTTGTGTTTATCACCTTTAAACACGTGTACCTTGGTGATTCAACGACGGCTTAAACGGTTATCAGGTAACATGCCCTTAACAGCATTATAGACCAGTTTGTCGGATTGTTTTTTCAACATATCACGACCACTTACCTTCTTAATGCCACCAATGTATTGGGAGTGGTGGTACCAAAACTCGTTTTCTTTTTTATTGCCAGTCAACACAACTTGATCACTGTTAATAATGATCAAGTAATCACCACAATCTTGGTTAGGGGTGAAATCAACTTTATTCTTGCCGCGAATAAGATCGGCCGCTTTAACCGCTAACTTACCGAGAACGAGCCCAGCAGCGTCGACAATATACCACTGTCGTCGCTTGTTGGCCTGTTCTTTTGTTAGCATTGATGTTTTTTGCATATTAACAAAACTGCTTATTATTCTAATTTATTACTTTTCTTCATAAAGCCCTTTGAAGAACGTATCAGCGATCTTCTGTGCCGCTGTTTTTTGAGCTTTTTTAACAACGGTACTACTTAACTGTTCTAACTGTTGTAATTGCAACACTTGGGGTTTATGCGTTAGTTGTTCCACTTTAGCTAGTACCTTAGTTTCATATTCACTCAAAGTAAAGGAACTCAAGAACAGGTTTTGTTGAAAAATTTCTAACAAGGTGGCTTGGGCAGTCGCTTCGGTAAGCGCTTCCTTTCACAACCTTGTTGCTATCTCTTTATCTTCGAAAATTAAGACTAAACCAAAGGGTGATGCTGCTAGAACCTTAATAGCTTTACTTAAATCACTAGCGGTAAACTTATCGGAAAACTGCTTTAAGTAACTGCTGTCTTTAGTTTTTTCTGCTTGGGTGAACTCCCTTTTAAAGCTGTCCAATAACTGTTGGAAGTGGGCTACTAGTTTAGGATCATTGTGCCCATAAACAGAAACGGCAATCACAGCCCGGTTAAGTTTTATTGGGATGGAAACTGGGTTATTATCTGATGGTTCTATTAACTTAATAGGAGCAGGCTTGCTTACAGGCTGTATTAATTTTTTAACAGCGGGCTGAAAAAAATCAGTGTTTTCTACAGAAAGAGGTCTACGACCGCGTTTGCGTACCACCTCTGGTTTAATCGCTAACCCAAATAAATCAGTTGCTGGAGCTGGTGAAGTTTCTGTTGGTTGTTCTACTTCTGTAATTTCTTGCTGTGCTTCTGGTTGCACTATAGTTTGGGGTTGTTCTATTAGTTTAGAAGCAACAGGATCTTTTACGAATGATGAAACTTTAGGTTCTTCTATTACTTTATAAAGAACAGGTTCTTTTGCTGGTAGTTGGGGCTTGGCCTCTTCTACAGTTTTTGAAGGAACGGGTTCTTTTGCTGGTGATTGAGTTTGTACCACTGGCGTATAAGCGGGTATGTGTGGCTCTTTGCTAACGGCATTTTTATAGTGAATCACAATTGCTTTAATCTGATCACTTAAGGATAGTTGTGAAGGACCATAACCATTGTTAGCTTCAATTGCTTGAACCAAAAGAGCATACTGTTTGGGGTGAAAACGAAGCTTTGCCGCCATTGTTTGGTCGCTGGGTTTTACAACTGCAATTTGTCCCGTTTTGACATAACCATATAAATCAACTGTTAGGGCAAACAGTTCCCTGAGAAAGTGAACAAAGTTAATCCCTTGACTTTCAAAGTTGTCAATTAAGTGAAAGGATTGTTTGAGATCACCACTTAAAACTGCTTCAATAAAACCAAACTTTTGTTCCTTGTCAAGCAGGTTAAAGGTCTTTTCAACATCAGCTAAACTAATGGTTTTACTTTCACTAAAGTTACTAATTTGGTCCAACAAGCTCAACCCATCACGCAACGAACCTTGCGCTAAATCAGCTAGTTTGACCAAAGCATCAGTAGTAATCTTAATGCTTTCCTTAGCCGCTACTTCCGCTAACCGTTGCTGGATGAGATCGTTAGTAATTTGTTTAAAGAAGAAGCTTTGGCAACGCGACAAGATCGTGATCGGAATCTTGTTAAATTCGGTGGTGGCAAAAATAAACAAGACATAATCGGGTGCTTCTTCTAAGGTCTTTAAAAGCCCACTCCAAGATTGGGGGGTGAGCATGTGGGCTTCATCCAAAATGTAAACCTTCTTTTTGAACTTAAAGGGTAGGTTAAAGACATTTTCCGCTAGCTCCCTAATGTCGTTAATCCCGTTTTTGGAAGCAGCATCAATTTCAAACACATCAATCGCACTGTTGCTGTTAATCGCTTGGCATGCCTCACACTGGTTGCAAACATCGCCGTTCCAGTTTAAACAGTTAATGGCTTTGGCAATAATCTTGGCAAAGGTCGTTTTTCCCGTACCCCGCTCCCCCGCAAAGATGTAACCGTGGGGTAATTGGTCTTGGGTAATGGCATTAACAATAATGCGTTTAATGCTGTCCTGACCGACAGTATCGCTAAACTTGGTGGGACGGTATTTCTGGTAAAGGACCTTGCGCATGTTTAAGCTTACTTTAAGATTTTAGCGAGATACTGGGCAGTGTAGGACTTATCGACCTGATTTAACAGTTGTTCTGGTGTGCCTTGGGCAATAATTTGTCCACCTTTTTCCCCACCTTCAGGACCCAAATCAATGATGTAGTCCGCCATCTTAATAATATCTAGGTTATGCTCAATAATAATGACACTATCACCGTTTTTAATAATCCGTTGGATCACCGTTAACAGCTTATTAATGTCTTCTATGTGTAGTCCCGTTGAAGGTTCATCCAGGACGTACAAGGTTTTACCAGTTGCCTTTTTCTGGAGAAACTTGGCTAGTTTAATTCGTTGTGCTTCCCCACCTGACAAGAAGGTAACATTAAGACCTAACTGGAGGTATTCCAGACCGACATCACAGAGTAGTTTTAACTTTCTCGCAATATTCGGAATTGCTTTAAAAAAATCATAAGCTTCCTGACATGACATCTGTAACACATCAAAGATCGATTTGCCTAAGTACTTAACTTCCAGGGTTTGGGGATTGTACTTCTTGCCATCACACATTTCACACGTCACATAAACATCGGGTAAAAAGTGCATTTCAATCCGAATGACCCCATCACCAAAACACTTATCACAGCGCCCACCGGCTACATTAAAGGAAAAGCGCGAGTTAGTGTAACCCCGAGCCTTGGCTTCCTTAGTGTTGGCAAACAACTCCCTAATGTCATCAAATACACTGACATAGGTAGCGGGGTTAGAGCGGGGAGTGCGACCGATCGGATCTTGCGACACCACTACCACCTTATCAATGTGGTTGACCCCTATGATTTCCTTATAGGGTGCAGGTTTAACATTCTTATAGTTCACCAAGCGTTCCAAGATCGGTACTAAGGTTTGGTTAATTAAGGTTGATTTCCCTGAACCCGATACCCCGGTCACTAGTACTAATTTATTTAAGGGAATAGTGACATTAATGTTCTTTAAGTTGTTGCCCTTGGCTCCTTTTAAGGTTAAGGTGCGACCATTACCAGCATGCCGGGTTTTTGGTACCTCAATTTGCTTTTTGCCACTGAGATACTGTCCCGTTAAGGAGTCAGTATTTTCCATTACCTCTAGCGGTGTCCCAGCAGCTACTACTTCCCCACCTTGGTTACCCGCTTTAGGACCAATATCAATGAGGTAATCAGCTGCTAGCATGGTTTCACTGTCGTGTTCTACCACGAGTAAGGTGTTGCCCAAATCCCGCATTACCATCATCGTGTTAATTAAGCGATCATTGTCCTTTTGGTGTAGCCCAATTGAGGGTTCATCCAAGACATAAAGGACGCCAGTGAGTTGCGAACCAATTTGGGTGGCTAAGCGAATCCGTTGTGCTTCACCACCTGACAGGGTAGAAGCCCGGCGCGCTAAGTTAAGGTATTCTAAGCCCACATTCTTCAAGAAGGATAAACGGTTAATAATCTCTTTAAGAGCTAACTCACCAATCTTCTTCTGTTCTTGATTCAGTTCTATTTGCAACAAGAATTCAATTGCTTTATCAATCGAGAGTTCCGTAAAGCTAATAATGTCCAACCCACCTAACTTGACACTTAAAGAATTAGTAGTAAGCTTCTTGCCCTGGCACTTCTTACAGGTAATTTCAGACATGTAAGCCGAGTACCATTCACGACTGGCTTGACTACTAGTCTCTAAGTGACGGCGTTTAATTAAGTGAGCCACACCCTCATAGTGTTCAAAGCGCACATTTTTGGCACCTGAGTTTGATACTGTTTTAATCTCAATTGGTTCATCACTACCCTCCAAGATCATCCGTACTAAATCAGTATCCATTTGACCTAAGGGTTGGTCTAGGGGAATTTGATAGTGGTTAATTAAAGACAAGAAGCGCTGTCAATCTAGTGATGTACCGTGCACTATGTTCTTAAAGATGTCAATACCACCTTCATTGATGGACAACAACGGATTGGGAATAATCTTTTCCACATCCGGTTCATAACTAAAACCTAAACCCTTACAGTACTCACACGAACCTAAAGGGGAGTTAAAGGAGAATAAGCGTGGCTCTAGTTCACTAATTGAAAAACCACACTGATCACAACCGTGGTTCTTATTAAAACTAAGTATCTTACCATCTTCCAACAACACTTCAATCCGACCCTCAGTTAGTTTAATCACACCTTCAATCGCATCAACAATCCGACCCAAGGTTTGCTTGTCTTGGTTAATGACAATCCGGTCCACTACCACACTCAAGTTGTGCTTGGTGTTCTTGTCTAACTTAATTTCTTCATCTAAGGTATAGACCTGACCATCAACCCAAACCCGGAGAAAGCCTAACTGTTGGTATTTAGCAAACTCATTAGCAAAGGTACCGCGTTGGTTTTTAATGGTAGGGGCTAAGATTTGAATCCGTGATTTAACCGGTAAGTTAAAGATCTGGTTGGCAATTTGGTTAACGGTTTGGGTTTGAATGGCACCATGACCATTAGGACAAAAGGGTATACCAATCCGCGCTCACAGTAAACGGAGGTAATCGTAAATTTCCGTTACTGTTCCCACAGTGGAGCGGGGATTGTGTGAAGTGGTTTTTTGGTCAATTGAAATGGCTGGGGATAACCCTTCAATCGAATCTACATCCGGTTTATCACTGTTACCGAGAAACTGTCTGGCATATGACGAGAGTGATTCCAAATACCGACGGCGGCCTTCCGCGTAAATGGTGTTAAAGGCAAGTGATGATTTACCCGAACCCGACAACCCCGTAATAACGACAAACTGGTTTTTGGGAATATCGATGTTTACATTCTTCAGGTTGTTTTCACGCGCGCCCTTAACGCGAATAAAATCATTAGTTTTTAGCTCTGGTTTCAAAGTTAATTGTTTGGGTGAAAAAATCCTTTACGAGGTTATTGTGCGGATCGTTAAATAAATATGCAATGCCTTGTTGGTTTTGGTGGGCAATTAACAAACCACGGGTAAAGTTATAAGTGTGTTTGTTAAAGTTGACAAAGGAGTCGACCAACACCAACTGGGGGTGTAACAATAGCTTAAATAACAGGTAAAGTTTAAAGCGTTGCTTGGTGTTAATTTTATTTAAATCGCTAAAGATCATTCAGCTCGGAATACCGTTTTTAAAGGTGTTATCCAACAGCATTTTGAGGTTAGCAGTACGGTTTAGTTTTTTGGGATTTTGCCGGCACTTTTTGAGACAAAAACTAATGACATTCACCATCGCGTTTAAGAGATTAAAGTGCAGCTTATTCGATGCCTGTAAACGGTTTAAATCACACTCTTCTGAATCCACAAAGAAGGTTTTGCGTACCACGTTATTGACTTTACTAATTTGAGTATTGAGCAGCTTATTTAAAGTGCCATAATTTTTTAGCAGTGCTTTAATTTGCTGTTGCTTTTGCTTAATTTCTTTTTTGTACAACCACTGGATTAAACGAATGTCTTGTAAAAGGAGGTGGTAGTGTGCCCGTCACTGTTTGAGCTGGGTTTTTAAGTTAAGTTGGTTTATGGTAGTGGTGTTTGCTTCTGTTAAAGTAGTGGCATTACGATTAGGTAACAACAAGTAAGCTTGAGGGTGGTGCAAGTTGGCTTCGAAGTATTTATTAACTGTTTTATCCACCAGTAACTTTCACTTCGGGGAATTTCAATCGCGGTTTAAGTTGGCCTTTAAGCTGGCAAACTGAGTTTGATTCCGACCTTGGTTAAAGACAAAGTCAACCACACCATGGGTAATAAACTGGTATTTGGTAAAGAAGTGTTGTGTTTGTTTGTGCAAGTTAGTTCATAAAGCACGGCACACAAAGTAGAGTTGGTTCAATTCGTATTTCGTTCAATCTAATTTTTCGTAAGTAGTTAAGAGTTGTTCAAAACAGCGTTCTTGAACAATATTCATTAGGTTAAACGGATCTAAGGTGTGTTTTAACTTAGTGTAGTTAAGCGCTGCTTTAACTGTTTGGATAAAACCCTTAAAAACCGCCTTTTGGTTTACAACCGAATAGCTTTGGCTAACCTCACCCTTATACTGGTTTTGGTTTAACAGAACGTTTTGCTTTAACTCATTGACCAGCTTGTTGTGTTGCTCACAGCGATTTAAGCCCTTAGCGTATTCTTGCAAAAAAGCTAAACGAGATTTAATTAAAAAACCAATCACCGCATCATGAGTCAAAACAATTGGCTTAGTTAACTTTTCCAATTCAAAGCTAATTGCTTGGTATTTTCAGTTAAGTGATTGACCTGGTTTTAGCTGCAAAATTTGCTGGTACAAGCGCTGGATGTTGCTCATTAACTGTTCTACTAGGCGCTCACTTTCAGCAATTGTGCTCACTAACAAATCGGGATTAAGTAAGCGCATTTGTTTCAGTTCCTTTAACAGTCAAATTTTGATTTGGATTCGCTTGGCTACAATCCGTTTTTCCAAAATCCGGGTTTTGTAAAAGTTAAAGATACTCTTGAACTTAATCTTGGTTTGACTCCACCACAAGGACGACATAAAGCGGTTAATCAGCTGCACCGAATTGCGCTGCTGTTTTTTGAAGGCACGCTTAAGCTGATTGGCTTGCTGTTGTGCACGGGTAATTTGCAAATTTTCAAAGTAGGTATTAACCCCGTGTTTGGTTTCATAGCTTTGAACTTTTAAATTGTGCAGTAAACGTTCTCGTTGTCTTGTAAGCGCATAGTACTGTTCAAAAAAACTTTTAGTTAAGTGATCAGGTTGTTCAAAGAAGCTGTTAAAGAAGTGGGTGCTAAATTCCACTAGGTCACGTTCATGGTCTTCCAATAAACCAACAAAGTTGTTGTACTCAAACTGGATCTGGTTAAAGTACACCTTATTGACAAACTTAGTGGTGAAGAACTGACTAAGTGTTTCACTCGTTAACTTATTGGTGTTATCTAGTTCAAACACCACAAACTGGCTTAACTTCTTGAGTGCATTAAAGCCCAAAAATCAGCGCAACTCATTGGGAATTTGCAGTTTTTCAATTAAACGCTGTCGTTTTTTAGGACAAGTACTTTGTCTTAAAAAATCATGAATAGTTTGGTGAAACGGATGGCTTTGTTGCACCGCGGCCAGTTCAATTTTTTCACACAAGTTGGTTTTAACGCGATGCAGCGGAATATAGTTGTGTTGATCCGTTCACAAAAAACCGGAATGGGGCGCTTTTTTGAGTTGTAAAACACTCGACAACGTGTTTCACTGTGCCTTATTGAGGTTATTAACGAGGTAGTTACCGTATTTATGGATGCTGAGGTTGGTGGTTCAGAAGTAGTTAATGTCCAAAAAGGAGTTATGGACCATTAACAAGGACAAATTAACTCACGGTTCTTTCTTAGTGGTAGTGGAAATCTGTTTCAGCATCCCTTAGTCTTATTGATTTTAGTACTAGGGTCATCCCAGTAACAACTAAATAGAAATAATAGTTGACAGGATTAGTGGTGCTTTAGCAGTCATTTTGGTAAATAAATTCATGACAGCTCGTTTAATAGAAACCTTGAGTTCCTTCGATGTGTCTTTGCCTAAACGTCGCTTAATTGTCGTAAAGTCCTTAATGTCGTTAATTTGTTTGCGAATTAACTCTTCCATTTTGGTCTTGAGTTTAACCTGATTGTTACTATCTAGGCTAACTCCTAAAAAGGAATAGGTAATTTCGTTTAGGAACTCACTCTTCTTGGAGTCATAGTAGATGATGATGGTAACCACACCCGCTTCAGACATCTGATCACGCTCAAACATGATGCTGGCCCCTATTTCCTGCAAACCAGCGGAGTCAACACACTTAGGGTTGAGCTTTAATTCCCGTTTTTTGTTGTCAATTTGCTTGTGGTTAATGGCTAGGACTTCACCATTAAAGGGAATTAAGACCTGACTTTGTTCCACACCAGCTTGCTTCATTACCATGGTAAAGTTAATGAAGTCACGGTACAGACCGGATGTGGGAATAATGTACTTAGGTTTTAAGGAAGTTACTAAAAACTTCATGTCTTCATCCGATGCATTGATCGACAAGATTTCCCGACCCAAGTTGTAGTAACTCACCTCATTACGCGCGACATCGTCCAAGATTTGTGCTTCCAACTCTTCATACCCAGCTATGCGGGGAATCATGAAGATAAAGCTATCGGTCTTGCGATACCGTACCCGTTCATCCTCATTAGTACCAATCTTAAACAGCTTGGCATACAACTTATCTGGTGGCGCGGTTAAAACAACAATGGCGTTTGTGGAGTTGTTAATCTCTTCAATCGAAATAGTGTTTAAGTGGGTGTTGTTAAACAGCTTTTGGCGCACAATCGCGTTGAAGAGGTGGACAAATGAATGGGAGTAGATGACAAAGGGGCGGTTTTGCATCCGTGCAATTTGGGCCAAGGTCATCACACTGTAAGCGTTGGAATCATAACAAGCAGCAAATACCCGACCCTTAGCGGAGGCAATAATGCGGTTGAGTTGTTCCAAGGATTTGTGCTTAGGGGTTGTAAAACCAGTGTTACGTCCCACTAGACCAACTCCAGTAATTAACAAAAGGGTCTTGTTCGCTACTTGGGGAATAATCTGATTGAGTTGGTTTTCAAAGGCGATGTTCTTATCATTGAGCACAATGAAGTCATCGACATAGACAATGTACCCATCATCAGTGTGGAGCGCAAAGCCAAACGAGGAAGGAATCGAACTCGATACCTTAAAGGGCGTAATGTTATGGTGCCCGATCTTGACCGTTTCTAACGGTTTAAGCTCGTGGATTTCCAACTCGTCATGGGGAATATTGAGCTTGTTTTCGTGGATCTTAGTTTTAATAACCACAGCGCCAATCGTACTGGTGTAAATCGGAAAGAAACCAACGGTGTGGAATAAGAACTCCAGCGAACCAATGTTTTCCGTTACTGGATTACCGATAAAGATGCCCTTAATGCGGGCCTGGTTTTCCTGGATCCAACTAAAGTCAGGGATGATCTTTTTGACCCCTAAAACAGCGGTTGTTGGTGTCAAACTACCAACGTTAAAAATAAAAACATCGTTATTGATTTCGAGAACAAAACAATTCTTGCCCCTCTCGTCCTGACCACCGAAGGCAAAGAAGTTAATTTTAGCCATGAAACTAACCCCGGAGATTTAAGGTTTTAACTAAATTTCTGTAAGCCTCTAAATCGTTGTGCTTTAAGTACTTCAACAAGCGCTTGCGCTTTGATACTTTCGCGTACAAACCACGTTTGGAAATAAAGTCCTTCTTGTTAGCTAGTAAGTGGTCGGTGAGCTGTTTAATTTGGCTCGTTAAAAGGGACACTTGTACCTGAATACTACCCACGTCCTTGTCGTGGAGTTGTGCGGATTTAATGATCCCGTTTTTATCAATTTGCATGAATTAATACTTGTTGAATTATAACAAAATAAGAAATTAGCCCAGTCAAGGGGTGAACCCCACAAAATCAGCCCCCAAACTTATAATAAATAAACCAAAACAACCACCTTAAGTGGGCTGTTAACTAATTAATTACTAATAACTATCAAAGTCATCTATGGACTCAACTTTTAATGAACTAGGCGTCTCTCCTGCCTTAATTGCCACACTCAAAGATAACAACATTAACCAACCAACTACGATTCAACAACTGGCTATTCCCCAGTTTCTCCAACATCAAAACCTGATTGTCCACTCCCCCACCGGCACTGGTAAAACCGCTGTTTTTGCCATTCCGGTCATTGAAACACTCCTTAAAAAACCAAGTAAAGGTACGACCCAAACATTGGTAGTTGCACCTACCCGTGAACTGGCCGAACAGATTAAAACCACCTTTATTAACTTTGCTAAACACACCCATTTAAAGGTAGTGAGCTTAATTGGTGGCATCCCCATTTGACAACAACTCAAACAACTAGAAAACCAACCAGAAATAGTTGTGGGCACTATGGGGCGGGTGATGGACCTGTTAGAACGGGGTGTAATTAAGTTTGAACACCTAGAACACCTCATTATTGACGAGGTTGACCTAATGCTCGACCGTGGTTTTAAGAGAAAACTGTTTGATCTGTTAGGTCGCATTGAAAAGTTTGAACAAATTGCGGTTTATTCTGCTAGTTACAATGAAGAGACAATTGAAACAGCTAAACAAATTACCAAGAATGGTATTTTCCTAGCAGCACCAGAATTAAAACAAAATGCCCCTGAACCAGATAACAAACTCATTGATCAATTTGTTTGTTATCTTTTTTCTAACCGGAAGAAACAAGCCCTCTATTCTTTGGTATCGCAAACTAGAGCGAAGTCGATTATTGTTTTTTGTGACACCAAGAAGTTAGTTGATGAGCTGTGCATCTTCTTGCGCAAGAATGATGTCAAAACTTATCCACTTCACGGGGACAAAGCGCAGTTCATTCGCGAACGCAACTTAAAGCTGTTTGCTAACACAACTGCTCCTATAGTATTAGTAACAACTGATCTAATCGGTCGTGGTATCCATGTGGAAGGGGTGGACATGGTAGTCAACTATTCCGCTTGTGTAAACTTTGAAACTTATTTGCACCGGATGGGTCGCACAGGACGGAACAACCACAAGGGCTCATGTATTACCTTCTGTACCTCACACGAAAAGCAAGCATTTTTAAAGTTATTAGAACAAGTTAACGACAAGCGCATTAGCCCTCTCCGCCCCATGCGCTTAAGGTTAATTCCCTTGAAGTGCAAAACCCAACCCAAGAAGGGGAAACTTTCCTTACAGTCAGTGCAAAAGATTTACGTCAATCCTCGTTCCAATGGCACCTTTAAACGCGTACCTTTAGCGGGGGATTTGTTTAAAAGCCGGATGCGTCAACCTGAGCGGGACATGCAAAAGAACAAATTGCATGACAGTGATTGACAAAGCAATATGTAAGCTTTGTTACAATTATTTGATATGGCAAAAAAAGACCAACTTACTTTAAGAGGGCCTTTGTATGGCAACAATCGTTCCCACTCCAAGACTATTACGCGCAGAAAGTGGAATGTTAACCTCCAACCATGCAAGGTCAAAACTGCTGATGGCAAGACCACCAGAATCTTAGTTTCTACCAGAACACTGCGCACCTTAAAGAAACACAACCGCCTCTCTTAGTGGCGCGCTAGAGTTAAATTAAACTTAAATCCATATAGTAAATATGGACAAAAAGTACGACATCACTGCTGTTTTAAACGAAGACAGCTCCATGACTGCCATCAGTGATCAGTTTCAAATTACACTCGATGCCCGTCCCAAGCACACCGCTAAGGGTTTTGGTCCTTTAGCTGCTCTGCTCTCTGGTTTAGCTGCTTGTGAATTGGCAACCGCTAACTTGATGGCACCAGCTAAAATGATTACCATCAATAAACTGTTGATGAATGTCACTGGTTCACGCTCAACCAACCCCACCGATGGTTATTTTGGTTTAAGGGAAATTAACCTGCACTGGGAAATCCACTCCCCTAACAGTGAAACGGAAATCAAGGAGTTCATTGACTTTGTTTCCAAACGCTGCCCAGCGCACAATACGCTCCAAGGTGTGAGTCAACTCAAGATTAACGTTAATGTAACTTTAGTGCATTAATTGGGTAAAAAACGAGCGAAAAATGCTGAAAAAATACCTGAGTCTTTCAGGTATTTTTTTATGCAAAATTTGCCCTAAAAAAAGAGTAAATTTGAGAAATTGATCCGTTGTTCATTCCGGGATAATTTTCCCGTATGAAACTACAAATTTTTAATACAAAGCTAGGATTACTAGCAGCTAAGATTTACTGAAAGTCCTGGAGACACCTAGGACTGTCTGAAGATGAAATTGTGTCAATTGCGCTCCACGCCGAGCATGACTCCAAGAAGCGCTATGACCCCAAATACGGTTTGAGCTTTGAGACCTACTTGAAGTTAAATGGTGCTAACTTCATTAAGTCGAGTTACCGTAGCTTAATGAATAAGGTTGATGACTGAATTGACTTTAAGGACATGAAAACACTCGAGGAACAAAACACAGTCTCTTACACTCCTGAAAACTATTTAAGAAGTGTAGAATTCCGTGAAATGGTGCACTTAGCGTTTAAGAAAGCCAAGAATGAGGATGAACGTCAGGTGTTTGCGCTTTATGTAAAGGGTTACAAAAATTTAGAGATTGCTAAAAAACTCAACCTAACGACCCGTAGAGTGCGCTACCTAATTTCAATCTTTAAGGATCACATTAAGGTATTGACTAAACGGTATGGTTACTAGATGAAGATATCCTTCTTCTTGAAGAATTTTTCCACTAGCTTTTCCACCTCCTTGGCGTTCGCTAAGGTTAATGCTTTTTGTGCTAATGTCTGACACTCTGTGTAATTGAGCTTAGCGATAATCATCCGCGCCTTAAACATGGAACTAGCACTCATGGAAAACTCTTTGAGACCCAAACCTAAAAGTAGGGGAATCGCTAACGGTTCACCGGCCATCTCACCACACATTCCCGTTCAAATATCATTCACTTTACCCCCTTCAATGGTGAGGTAAATGAGTTTTAATAAAGCTGGGTTTAAAGGTTGGTAGAGATAACTAACATTCTTATTCATCCGGTCAGCCGCAAAGCTGTACTGGATCAGGTCATTGGTACCAATGGAGAAGAAATCCACGTGGTGACTTAAGGTGTTAGCCGCTAAAACAGCAGCGGGGATTTCGATCATAATCCCGAGCTTAAACTGTTTATTGCCACCCAACTCCTGGTGCACCTGGTTTAACAGTTGCTTGGCTTGCAACAGTTCATCCAAGGTAGCGACCATCGGGAACATAATGCCCAACTGACCGTGATCAGCAGCACGTAGTAAAGCCCGCAATTGTGTTTTAAAGATGTCCTGTTTGTCTAAGGTTAAACGCAAAGCGCGGTAGCCCAAGAAGGGGTTATCTTCGTGAGGAAACTCATAGTAATTAAGCTTCTTGTCCCCACCAATGTCTAGGGTGCGGATAATCACTAAATCACCGTGCGCTTGTTCTAACACTGCTTTGTAAGCCTGGTACTGCGTTTCTTCATCGGGTCATTCTTGACTGTTCATGTAGAGGAACTCAGTGCGGAATAAACCAACCCCGTTGGTATTGTACTGACATGCCAATTCCATGTCTTTCACATTACCAATGTTAGCAGCCACCACAACCGCATGACCATCTAAAGTCACGGTTTCCTTGTTGGTGTACTGCTTGAGTTCTTGTTGGTATTTTTCTTCCAACGCTTTTTGTTTCTTCCACTCGGTAGTGTCCTTGGAGTTTAGGTCTAAGCCAGCTATTCCTTTGCGCCCATCAACCCCAACTATTTGACCGTCTTTGACCTTAGTAGTAATGCCCTTAATGCCCACCACTGCGGGAATTTCCAGTGAACGCGCCATAATGGCAGCGTGACTGGTACGACCACCTATTTCGGTTAAAAAACCCTTGACGTATTTTTTGTTAAGGGTAGCAGTTTGACTCGGCGTTAAGTCATGGGCCACAATAATGACATCACTCTTAATCTTGACCAGGTCATGTGGTTTTTGTCCGGTTAAATAAGCTAATAAGCGTTGGCGCAAGTCCAAAATGTCTGCAGCACGTTCCTGGAAATACTTGTCCTGCATTTGGCTAAACATGGTAGCCGTTTGGCTAAAGACAGTGTCCACGGCTATAACAGGATGAACATTTTGCGCTAACTGTTGTTCTAGTTGCTCGGTGATCGTGGGATCGTTGAGTATTTGAATGTGCGCATCAAAGATCATGCCCGCTTCAGTGTTAATGTTGTTAACGGTAATTTCCTTAATTTCCTCTAAGTCCTTAACGGCTTTTTTGAAAGCTGAGTCCAAGAGTCTTTTGGCCTGCGCCTTCGTTAATTGGTGCTTGAGGTATTTGTTAACAGCGAATTCCGGTGTTTTAACAAGGAAGGCTTTGGCTAGGGCCATGCCATCAGATACACCGATACCAGATAGTTTTTTCATTTGTTTACTTTTTACTTATTAAAAGGGAGTTTGCGGTCATTTCTGCGGGCTGTTTGAGCCCTAAGTAAGCCAAAATGGTTGGGGCAATATTGGCTAGACTAGCACTGTCTTGTTGTAAGTCAATGTTTGTATCAGTACATACAAACGGCACCTTGTTGACAGTGTGTTTGGTAACTGGGTTATTGTTGCTGTCAATCATCTCTTCCGCGTTACCATGGTCAGCAGTTAAGAACAGGGTAATGTGGTTAGCCTTGCAGAAATCAATAATGCGCTGTATTTGCACATCCAAGGCCTCTAAGCCCTGCACACAAGCGGCATAGTTACCAGTATGACCTACCATATCAGGGTTGGCAAAGTTCAACACCGTCAAATCGAACTGGTTAATTTGATTTAGTAATTGATCAGTTATCCCCTTACATGCCATTTCCGGTGCTAAGTCATACGTAGCCACCTTTAACGAAGGTACCATGCACTTAGTTTCATTGGGTAGATCCACTTCCACTCCACCATCAAAGAAGAAGGTTACATGGGCGTACTTTTCCGTTTCTGCAATTCGCAGTTGCTTTAACTTATTGTGCGCAATGACTTCCCCAAAAGTATTGGGAATAGTTTCGGGCGGAAAGACCACGGCACTTGGTTTAATCCCCTCATAGTTCATCATCGTGACAAAGAAGAGGTTGTGCTTTCGTCCTGCTTTAGGGGTGTAGTCATAGTAATCTGTTTGAAAGAGCATGTGTGACATTTGCCGCGCACGATCAGGACGGAAGTTAAAGAAGATCACACTGTCATGATCTTTGAGCGCAAACTGCTCTTTATCTACATTACCATTGACAGCCGGGTACAAAAACTCATCGGTTACCTTTTGGTCATAGGCACTTTGAACATAAGCTACCGGATCAGTAAAGCTAGCCTTACTGTTACCCAAAATGGCATTATAAGCAATCTCTTCTCTGTCCCAGCGCTGGTCACGATCCATACCATAGTAACGCCCACCTAAGGTACCAATGATGACATTGTGGTAATCCTTTAAGAACACCATGAAGGCTTCAAGGTCACTCTTCATCGTGCAAGGTGCTACATCCCGCCCATCACCAAAGAGGTGGAGCACCATTTTGACCTGCGGTGCTGCTAGTTTAATCAAGGCATACAAATGGTCCATGTGACTGTGTACCCCGCCATGGGAAAACAGACCAATTAAGTGCAACTTAGCATTGTTAGCCTTGGCCCTAGCAATCGCCTCTAGTAACACCTGGTTGTGGTGAAAGGCCCCGGTTTTAATGTTTTGGTTAATTAACGATAAACCAGTGTAAACAATTCGACCAGCCCCAATATTGAGGTGACCCACTTCCGAGTTACCGATTTGTCCTTGTGGTAGACCAACGGCTTCCCCCGAAGCTTCTAACATGACACAGGGATAATCCTTAATCAAACTGTCTAAAGCTGGAGTTTTAGCATGGTACACCGCATTACCGTGTTGCTTATTACTAATACCATAACCATCCAAAATGGCTAGTAAGACCTTCTTATGCATATACTTGTGCCATTACTAAAAAGTCGTTGACATCCAAAGATGCCTTGCCCACTAAAAAGCCATCAATTTGCTCGGTTTGTGCTAACTCCTTAATGTTGTCCTTAGCAACCGAACCACCATAGAGAATCGAGGTTTGTTGGGCAATTTCCTTGTTGTACAGTTCACTAAGGTACACGCGAATTTGGGCAATTGTGTTATTAGCAATTTCCGGTGTAGCGGTTTTACCCGTACCAATTGCTCAAATTGGTTCGTAAGCAATCACTAACTGTTTGAGTAAGCTTAGGTCAATGCCTTGCAAGACTTGGGTTAAGTCTTCTTTGAGAAAACTAATTGGTTGACCTTCCGTTTCACCAATACAGAGCACGACTAACATTCCAGCTTGTAAACAGGCTTTTAGTTTTTGATTAATAACTTGCGCTGTTTCGTTAAAGTACTTTCGCCGTTCCGAGTGACCAATAATGACACTGTTGACCTTAATGTCTTGCAGTTGGGTATAGCTCACCGTACCAGTGTAAGAACCACTAGCAATAAAGTTAGCGTCTTGGGCTAACAGTAAGAGGTTATTAGGCAAGACTTTGTTTACCTCGGTTAAGTGGACGTGGACTGGGGCAATACCCAAAACCGCCTTAGTTTTAGCAGGGTGCTGTTTAAATTGCTCCACAAAGGCCAACGCTTGGTGTAAATCCTTATTTGTCTTTCAGTTACCAATTAGGTATTTCGTACGCATACGTTAAAGATTTTATAAGTTTTATTGATTAACCCCAGCGGGAAATACCTGATTAATTTTCCTGGGCTTTAACTGGGTCTTTTACTGGTGGGACTACTTTATCAGGTGCTTTTCTTTCATAGATATAACCATCCAACTTTTTAATGAAGGTAATTGAGAACAGAGCATCCGGATCGACTCGTCTAACAGCTTCTAAAAGTTCGGCCGTTTTCGTAAACAAACAGTTTGTAACTAACACCTGGTTCTTTTGGCGCGAATACCCCCCTTCCACTTCGAACATAGTGACCGCAAAGGACTGGTTACTGCTCAACAAGTAGTTGCGCATTTGTTCCATGTGTTTACCATACACTTCCACTTTGACAAACTGGTATTTAGGAAAGAAGTAGGAGGTAAAGATCCCTAAGATAATGTTCATGAAGATCGTAAACACTAAGTTAGGGGACAAGAAAAAAGCCACTCCAAACGGTTTTTGTCTATCTCCTTGAAAGCCTTGTGCTAGTAAGCTACCGGTAAGGTAAGTGCCAATGGTGTAACCGATCAAGAAACTAAGGGTGTTAACAATAAACAAAATACCACCAATGTCCTTGTGTTTCTTTTCCGAATACCAGAAGGCCAAAAAGTCCAACCCACCACTCGATGCATCAATAATTAGGATAACTGAGTAAAACACAGCTTGAAGAAAACCCCAGATCAGCGCATAGAACATTAACGAAATTTGCTTTTCAGCGGTTTGTTCCCAAAAGATCAGTTGCACCCCTTGGTTGTTAATCGCTTGTTCTAAGCCACCGTTGGCCTTAGTAAGTTCAGTTAAATTAGCAAACAAGAAGAAGTTTTCCACGCCCGGAATGTAAGAGAAGGCAAACCCAAAAACGTTGGAGACTACCACAAAGTAAAGGGTTAACAAGGTAAACTTTTTGGAAATCTTGTACCAACCCAAAACAAATAAGGGAATGTTAATCAGAATTTGACTCAACCAGAAGATGACGTTGTAAATGGTGTCAGCATCCACACTGACCTTGTTCGACCTAATTAAATAATCCGCTAACCGTGCTAAGCCCTGACTAATGGAAGCAATGCCAATGTCATATAATCCAGAAAACTGGACAAACACAACCCCTAACAAGCCCCAAAAGAAGGCAATCACGGAGAGAATTAACAGCTGTAAGTACAGCTTTTTCACCTTGTAAATTGCCTGGAACTTCAGAAACGATCCCGAAAGGTGTACCCGTACCAGATTGCCCTGAATTTTGACGTTTTTATCTTGCATATTCCAAGTCCTCCTTTTTCATCATTTTGCCAATTGCTAACAGGCTTAAATTTAATATTTATTAGGTACAAAAATCGATTACATTAATGGCAACAAAAATAGAGCTCATAAAAGAACTACGTAAAACAACACAAGCGAGCATGATGGACTGTAAAAAGGCTTTGGAACAAAATAACGATGATCTCGAAAAGGCCATTAAGTGATTGAGAGAAAACGGAATTGTTAAATCTGCTAAGAAATTAGGCAAAGTAGCCGCTGATGGTTGCATTGTGTTACACAGTGATCACCACAAGGCAGTGATGGTGGAAATTAATTCTCAAACGGACTTTGTAGCGCGTAGTCAAGAGTTAACGGACTTTGCGCAATTAATGATTAGTGAAGTCTTTAAAAAGGCTACCCCTACCACTACTATTGAAGAAGTAACCCAGTACGAACTCCAAGGCAAAGAAAAGGTAGCAGAACGTTTGGCTTTAGTCGCTTCCAAAACTGATGAAAAGATTGTGTTGCGCCGCTTAATGGTGTTTGAAAGCAAAACTAACCACATCTTTAGCTACCTCCACGCTAACAAACGGATTGGCGTAATCTTGGAAGTGGAAGGCAAGTTTGATGAACAAGATGGTAAACACTTAGCAATGCACATTGCCGCTAACTCACCGCAGTTCATTGATCAGGACAATGTGGATCAAACATGGTTAGCTAACGAAACCAGCATCATTAAGTCCCAAGCCAAACTAGAAGTCCAAGACAATCCGAAGAAAGCAGCGTTCTTAGACAAAACTATTGCTGGACGCGTGAACAAACTCTTAATTGACATTTGTCTGGTAAACCAAAAGTACTTGGTCGATGAATCCAAAACAGTTGGTCAATTCTTAAAGGAAAAGAACAGTAAAGTAATCCATTTTGTGCGCTTTGAAGTTGGTGAAGGCATTGTCAAAGAAGCAGTTGACTTCGCTAGTGAAGTAAGTGCGCAAATGAAAAAATAAGATGCGCCTAAAGATCCTAATCAAACTAAGCGGTGCTGGCATGAGCGCTGATAGTTCCGAACCGTTTTCCAACCAATTTTTAGAAACTGTCATCGCTCAACTAAAACAACTAGTACCCAACTACCAAATAGGCATTGTGATTGGTGGGGGCAACATTATGCGCGGTAAGTCTTGTAGTGACTACAACATTACCGAAATAGCAGGGCACCACTTGGGCATTATGGCCACGGTTATTAACGGCGCTTTTTTAAAGGCTAAGTTTGATAGTCATAAACTTAACAGCACCCTGTTGAGTGCAGTGAGCTGTCCTAGTTTAGCGACCCACATTGTGTCCCAGGCCACGATTGATGATGCCTTTAAAAACCATGACATTGTCATTTTTGCGGGCGGTACTGGTAACCCGTACTTTTCGACTGATACCGCTGTGGCGTTGCGTGCTACCCAAATGCAAGCCGATATTATTCTAATTGGCAAGAATGGTGTTGATGGGGTTTACACTGCGGATCCTAAAAAGGATAAGCATGCTAAGTTTTTAGCTAGCTTAACTTACGCTGAAGCGATTAAGAATGACCTGCAAATCATGGACATTACCGCCTTTACAATGTGTAAGGAAAATAACTTAAAGGTGATCATATTTAACATCAATGCCGAGCATGCCATCATTAAGGCATTGAGCAAACAAGGTAAATACACCTTAATAGAAAAATAAGCAACTGTTTTTTAGATTAATAACATAATTAAAAGCTGGATCGCTGTTTAATCTCCTGCAGCTTTCCAGCTTTTTTATTAGCCATTTTCAAAATAACTTTCTATGCGCTACTTAGACCTCAATATTAAAAGCATCTTAGCTGATTGAGAAATTGCTGATGCGATCCGTGAATTGATTGCCAATGCGATTGATGAACACCGCTTAAGCAACACCGCTTTCCCCGTAATTGAATTGCAAAAAGGCTTTCTAAACTCTTCTTTAGTGATTAAAGATTATGGTAGGGGTATTAAGTCCAATCACTTTATCCAAAACGAAAGTAGGGAAAAGGTTCAAAGTGAAAAAACTATTGGTAAGTTTGGCATTGGTCTCAAAGATGCCATAGCTGTTCTTTTCCGTCATAATGTCAAAGTTAGCTTTACCAGTAGTGAAGGCACTTTTACCCCTGTTGAGCGAATGAAGGAAGGTATGAAAGACGGCACCAAAACAATTCAAATAACAGTGGATGAAACCAAGAAGATTGACAAGGGCACTGACATCCTCATTAGCAAGATTAGTAGAAGTGATTACGAAAAAGCCATTGCTATTTTTTTGGAATTAAGAACTGGCTATCAAAAACTGGCTTCTTCCAAAAAGGGTGATATTTATCGCAGTGAAAATGGTTCAGAGATCTTTCTTAACGGGATGAAAATCGGAACCGATGAAAACTTCTTGTTTTCTTATGACATTAAAGAACCCAATAAGAAATTGCAAAAATCCTTAAACCGGGAGCGTAAAACACTTTCCCGTGATAGCTACCGTGACAATATCATTTCCATTTTGAAGTCCAGCATTAATAAAAATACCCAAACTTTAATTAACCAATTAATTGACAACCGCGATCAGTTTGAAACTGGTGAATGGAGTTTTATTGATGTCAAAAAGTTAGTAATGCAAAACACTAACCGAAAGATTTTGTGAACATCCAATGAAAGCAGTGACATTGCAGCACCTGCTTATCAAACATGAGCACAAGAGGAAGGTTATGAAATTATTTCGATTGGTTCATCACAATATCAAAGTATGGAAAATGATGCCGAATTTAAAAGCTATACCCTCAACGATTTTGGTGATAGATTTGTCAATGAATTCCAAACGGAAGAGGTGCCATTCCACAAATTAACTGAGATCGAAAAAGACAACTGGAATTGGGTAATGGCTAAGGTCAAAGAACTTACCAGGGTTTGAAGTAATTGGAAAAATTTATACAAACACTATGAATTTTCGATCATTAAAAAACATCCTAATGCTGAAGGATTACATAGCAATGGAAGAATTCAAATAGTTAGAAAAATTCTTAATGAACGCTCTCACTTGTTCAACACTATCATGCACGAAATTTGTCATGCAACTAGCTTTAGTCCTGATGTATCAAAAAGATTTGAACAAGGATTAACCAGTGCATTTTATCCAGTTATGAAATTGAAACCAGAATAAAATGGTTTTCTTTTCTATAAATGTCTAGCTAAAACTTTTTTGCCAAACGTTTTTGACCTCAAGTAGAAGCTTGTTCAAAAAATCATCATGGCCTTATTAAAGTTTTTCAACCAAAGTATTGCCCTTCTTTTAATCCAATTGGGCAAATTGTTAAAATTCCCAGATTATGTTTTATCTCGATTTAAACATTCAAAATATTCTTACCAATTGGGAGATAGCTGATGCTGTTCGCGAACTGATCGCTAACGCCATTGATGAGCACATTTAACCGAAACCCAATTCCCTAAAATTGAATTTAACGAAAACAATTCAAGCTCAACATTAATAATTCAAGACTTTGGTCGCGGCATTAAAATCGAACACTTTATCCAAAACGAAAGTAAGGAAAAGGTTCAAAGTGAAAAAACTATTGGTAAGTTTGGCACTGTTTTAAAGGATGCAATTGCTGTTTTATTTCGCACTAATATCAAAGTGAGTTTTACAAGTCAGCAAGGTACTTTTATTCCTGTAGAACGGAGTAAAACTGAACTGGAAGGTAGCATCAAAACAATTCAAATGGAAGTTGATCCAGCTGAACAAATTGAAAAAGCGGGTACCTTAATCACAATTGAAGGATTGAAAAAAGATGACTATGACAAAGCTATTGTTAATTTCTTAGCTCTACGGGAAGATTTGCAATTATTAGCTGCTTCACCCAAAGGCGATGTTTACCGTAATACATCAGGTAACGGCGCAGAAATTTTTCTCAACGGGATGAAAATTGCAACCGATGAAGACTTTCTCTTTTCCTATCACATTAAAGAACCTAACAAAAAGTTACAGCGTTCCCTTAACCGGGAAAATAAAAATTTACCAAGAGATTGTTATCGTGAAAACATCATCACCATTCTTAAGTCCAACATTAACAATAGAACACAAACACTTATTGATGAATTAATAGATTCACGTGACCAATATGACAATGGCGAGTGGAGTTTTATTGATGTCAAAAAACTAATTGGTTTAAACACAAACAGAAATATTTTGTGAGCCGATAGCAGTAGTAAAAACATAGAAAAACTAATTTATTAACTTTATGGAATGGATACCAAAAAATATGAAATTTTGGCGTTAAATTCCTTGCAATACAGAAGCATGGAAAACGATGATCGTTTAAAAAAGCAAACACTGATGCATGTAAGTGAAAAGTTAAAACAACAAAGAATTGAAGAAGAGACTGAGAAAATCAAAGTTAAAGAACAAAAGCCACGAAAACGATTTGAGGAAGAAGATTTGCCAATAGAAGATTTAAATCCAATTGAAAGAGAAGGTTGGGATTGAGCGATGGAAAAGGCCAGAGAATTATGTGGTTTTATAAGAGGATGGGAAAAACTTTATGAAGAATATCAGTTTGTTCTAATGGAAAAGAATCACAAATATGTCGGGCTTTGTTACACCGATCAAAAAATTATCAAACTCTCAAGAGAAATATTGAAAGATGAATACAGTTTATTGAATACCTTAGTGCATGAAATTTGTCATGCAACAACTAATGGTCGAGACGGAACGAAGAAGTTTGAAAGGGGTCTAACTGATGCTTTTCATCCATTATTTAAACTAGGGCAAAGTAAATAATTAGTCCGTGTTTTACATTGTCGTTGAATTTCCGCAACCGCTTTCCATTAAAATAATTTAACGCCTGAATAAAGTGATGAGTCCAGAAAAATACCTGAACTTCTTTAAAGAAACCGCTGATAAAAAGTTCCAATGACTCAAAGAAGAATTGAGCAAAATCCGTACTGGTAGACCTAATCCAAAACTGTTTGATAATATCCTTGTGGAAAGCTATGGTGACAGAATGCCAATGGTAGCATTAGCACAAATTGCGGTAAATCCACCGCGGGAAATTGTGATCAAGCCGTTTGACGTTAAAAACAATATTAATGCCATCTACAGCGAAATTCAACGTGCTAACTTAGGTGTACAACCAGTGATTGACGGAGATAAAATTCGCATTAACTTTCCCCCAATGACCCAAGAAAGTCGTTTGGAAAGCATTAAGCAAGCTAAAAAAGTAGTGGAACAAATCCACCAAGAGTTGCGCAGTGTGCGACGTGATACCCTTCAAATGATTAAGAAGGATGATCATAAAGACGAAGACTTTGAAGAATTCTTAAAAGAAGAAGTAGAAAAAGTTAACAAGCAGTATATTGCACAGTTAGAAACTATTCAAAAACAAAAAGAAAAAGAACTATTAGTGGTCTAATGGATCTGAAAGATAATTCCTTCGCTAAAAAACGATCGACAGTGTTCGTTGTTTTGCTCATTGTCTTTTGTTTTTTTTTAATGTTTTCAGCCTTTGCTGACGGCTTTAACTTTTGATCGCCTTGGTCAACTGATTTCAATTCACGCGTAATTCACATTAAAAGTGACGGTAATATAGAAACCACTAGTATTGTCAGCCATGAGTTGCATCCTGACTTTAAGGCAGTCCGTTTTGCTTTTGGCTTAGTGATAGTTTTATTTATTAGCGTTATTGCCGTTTTAATGAATTGGGAGTTGTCTAACAGTATCTTTAAAAACTATGAAAAGCTCAACTTATCCTTATCACTACTTAGTGGAATTATGGTTTCAGGTGGGATGATTCCAACCTTTTTCGTAATCTATTTCCGTGAATGAAATGCGACCGTTAACTGAATTTGAACTGCCTCGTTGGCTGGCATGATTGTCTTTTTATGGGCAGTGTACATGATTAGCACTTCCTTCATAAAGATCCGACCATCATTGCAAGTGATTTATAGCCTAGGTGCAGTGATCTGTTTCATCGCTTGCATAGGTACAATTTACTTTTCCGTAATTAGGGGATGGACTACCATTTTTCTCTTAATTGCTATTGGCGTTTGCACTGATACCTTTGCTTATCTGTTTGGTAAACGTTTTGGAAAAAATCCCCTCATTAAAATTTCTCCCTCAAAAACTTGGGAAGGTGCTTTCTTTGGGGTTACTGGTACAGTTTTAACCATTTCTATTATTTGTGTTTTGTACTCGATCCCTAACTATGTAAGACAACCTAGCATAAAGGATGCAAGTAAGACAGCACTACAAACACCACAAAACTATGATGTGCATAACCTTATTACCAATGTCTTTCTCATCTCCTTCATTTCCGGTGGTAGTACCTTTTACATCTACTGGTGAGTTAGCACATTAGCACTGATATTTACTGCTTCCATTTTTGCCATAGGTGGTGATTTATTCTTTAGTTACATTAAAAGACTCACAAAAATTAAGGATTTTTCGAAGTTATTGGGTAAACACGGTGGTATCTTAGACCGGTTTGATTCCAGCTCTTTTTTAATTAGTTTTTTCTTTATTTACCACGTAATTGCGGGAATCTCTTCTAACCAAAGATTGCTTATGGAACCAAACACTTATTTCAGTGCTGTAAGTTAGTTTTTAGAATTTAAAGGAAATGACTCCTAAATTAAAGCTTAACACTAACAGTAATTGAACTAAGAAAACCCTTGGCAGCTTATTTGAACTCAAGAAGGGTGAAATGCTCGAAAAAGAGCTATTGGCGCCCGATGGTAAATACGAATACTTTAACGGTGGTATTAAAGCCTCTGGACGCACCAATGAGTTCAATACCTTTAAGAACACCATTAGCATTATCATAGGCGGTTCTTGTGGTTATGTCAGATTAGCCGATAAGGATTACTTCTGTGGCCAGAGTAGCTGTACCCTAACTGTTTTAGATCCCCTGGAAATTGACCTCAAGTTTGCTTATTATGCACTTAAATCACAAGAGGAAAAAATTACCAGTTTAGCTAGCGGAACTACCATTAAAAACATTCGCCTAAGTGATTTAAAGGATTTACCTATTCCGCTTGTTAAAAGCATTCAGGATCAACGAACTATTGCACATGCTTTAAGTGTGTTTGACCTTCGCATCGAACACCTCAATGAATTAATTGAAGTAAACCGTAAGCTCAGAGACGAGTATGCCCACAAATTATTTACCTTAGATCCAGACTTCTTAACACACTGAAACCTACATGAACTCCATGAACAAATGGGTGAAATTAGTTTAGGTGAAGTGTTTCACTTAAAGAGTGGTAAATACTTAAAGGCTGATGAGCGCTTTGAGGATGGCAAATTCCCTTATTATGGTGCTGGTATTGAAAGCACTAGCTTTGTCAATGAACCCAACACCAAAGGTGATACTCTTTCAATGATTGCTAACGGTTACTCCATCGGAAACATTCGCTACCATACTATTCCTTGGTTTAATGGTACTGGTGGTATTGCCATGGAAGCATTAAAACCTAATGAAACCTATGTGCCGTTCTTTTACTGTGCTCTAAAATACATGCAAAAGGATCTAAAGGAACGCTTTAAACGTGATGAATCACCGTTTATTTCCTTAAAACTAGCCGGTGAAATTAAGGTTCCTTTCGTTAAATCGTTTGCACTCCAAAGAAAAGCCGGTAAGATTATTTACTTGTTAGATAAAACTCTTGAAGAATACAAAGAAGAAGCTAAGTCTTTAATCAGTATTCGCGATAACTTATTGGGCAAATTGTTCCCAACACTATCTTAAAGCAAAGCAAATAAGGTGTAAGGGAAAAGGTTAACACCTTCGTTATCAAAGCTCTGGTCTTTGAGACTGCTGGTTAAGCTATTCTCCAATAACTTCTTCATTAGATCACTCGTACTAGAAACTTGAATTCTTAACTTTGTGTTAAGTTTGATGTTACTGGTAAAACGGTCAAATGAAGCGCGGCCAAAGATGCCTTGAGTAGCTGGACTATATGTAAAAGTGGCTTCTAGAGCTTCATTTTGACCCATACTCATGGTTAAAGAATTTTGATCTTAGTTTCATCATCCTTAAAAATATTCTCTATATGGCTCATCGCCTTGGTTGGATCAATTTTTAAATCTGCCTTAATCTTAACCGTAGCCTCAAATTGCTTATTCGATTCAACTTTGTAGGTAAACTCCTTCACTTCTTGCTTGCTAACAGTGATGGCTTGTTCAGCTAATAATTGAATCTCAGTTGTTGAACGTTCACTTAACTTTAAACCCTTAAGCGTAGCATTGGGTTGAACACTGTTTTGGTTACTACCGTTGCCGAAGGATTTAGCAAAGCGGCCTACTTCAGTTTGCCAACGTTCTAATATTCTCTGTGAAAGCAAAGTGTACCTTCAACCTTCAAACAAGAGGTTTAAGGTACCTTTAGGATTTAAGCCGTAACTTTCGCGTAAAGTTGTAATTAAGGTACGTTTATTTTTCGTTAGTTCTGTGTTGCGATCTGCTAGGTTTTGCAGTTCTGATAAAGATTTAAGTTCAACGCTGGCAACTAGAGCAGAACAACTGCTTAAAATAAGCGCTAAACTAGGGATACTAGCTCAAATTCACTTCCTTAATTTAAGCGCCATACTCCCTAACATGCAGTAATTAAACAATTGTAAAAATTACCGCAAATACTGTAAAAGGACTAAAAGAATTAAAGCAGAGCGTAGTAAAAAAACGGTAATAAATTAAATAACTTACCTGTTAATTGACGACTTTTCATCTTTTTGACCTGTTCGTTGCTTTCTAATAATTCATTCAGCTTTGTGTGAGAAGTTGTTACATCAATCTTGACATTAGCGGGAAAGTTAATCGAACTAGTTGGTACATCAAAGGATTTATCAGAGAAGTTTCTAGCACCTCCAGTTGGTGGGGTATAGGTAAAAATAAAGTTACCAGTGAATTTGGCTTTTTCACCAGTGTATTCACTTTCATCAATACTGTTTTTACCATTGCTACCTTTACCTCCCGAACTATTACCCTCTAAGAATGATTTAATCAATGCTTTGCTTGATTCATCAATCTTTCCATCCATTTTGATTTGAATATTTACCTGAAACTTTTTTGGACTGTTTCAACTGTAAGAAAAACCACTAACTGTTTGCTTTTCAACTTTAAATACTTTAGCGATTTCTGAACTAATACCAGGAAAAGTGTCTGCTAAAAATAAACTTTTTTCACTAATGTTAGGGGTAATATCGTCCTTCTTGCTAGTAGAACCAAAAGCGCTAGCAAAGCGTGAAGCATCTAAATTTCTTTCTAAAAGCTTTTGATCCATTAAGGTGTACTTTCAACCTGCTAAAAGAGCGTTAACTGTTTTGTTAGGATCAGAGTTATAGCCATCTTTCAGAATGTTGATAACGTTCTTTTTGTTCAATTCACCTTTATTTGCTTCAAAGTATTTTTGCGCGCTACTTAAACTCGATATAACCTGGCTAGTTGCAGAGGAACAAGCGCTCAGCAATAAACTAGATCCTAATAAAGGAATTAATAACAGTTTAAGTTTCATTAGAGAATGGAATAAAGCAAGTAAGGTAAAAGATCAATTGATTGGTCTTGGAAAGTTTGACTGGACAAATTATTAGTAAAGGTAGTACTTTGAAGAATCTTTTTCATGATTTCGTCTTTACCAACCATATCTATTTTTAAGGTGGTATTGAAATTAATTTTCTTCATTAAAGTACGAAGTTCTTTCTAGAGAAAAGATTTGATTCAGGTACAGTATAGGTAAAAATAAGATCTGCAGAAAGAGTGCCACTTTCTTGTTTCAAATCACCATCATTTAAACCAATTTGTTGTAGATAAGATTGAACTTGACTTTTTTGGTATTTTCCTTGAGCATTAAGTTTTACTTGAACTTTAAAACTAGTTCGACTTTTGTATTGGATATTAAATGCTTCTACTTTTTGCTCATTTAGCGAAATAACATTGTTGATAATATTAGCAGTGCTTGGTTCAAATCGTTTATCAAGTCTTAAACCTTTAACACTAGCATTAGGCTCAACATCTTCATTACTTTTTCCAGAGCCAAAAGCTTTGACAAATCTAGAAGGGTCTTTGCTCTCCAAAATTTTGGAATCTAATAAGGTAAATTTCCAAGCGTCTAATAAAACTTTGGTAGTTCCTTCAGGATTATTTTCAAATGCTTTTTGCAGTGACTCCACAAAAGCTTTCTTGTCTTGGGTTAGACTGGTATTTTGATTAACGCCTTCCCTCAATTGATTCAAACTTGTTAAATTAGTTTGAAAATCAGAATTAGAACAAGCAGTTAAAGCTGTTCCGAACAACATGAATAAAGCACCTAATGAAGCTTTGGGTAATAGTCTTTTCTTATTCATCTTTAAAGTAGAGCATAGTAAAAGAAGGGTAGCAAGTCAATATTTCTTCCATTAAATGTACGACTTTTCATTCGCTTAACTTGATCGTTTTTCTCAAGCAGTTCATTTAGTTTATTATGCGAAGTGTTCACATCAATCTTTACATCAGCAGGGAAATTGATCATATTGGTGATAACATCAAATGATTTATCGGTTAAAGATCTAGTACCTCCAGTTGGTGGAGTATAGGAAAAGATAAAGTCTGCAGTGAATTTAGCTTTATCTCCAGTATATTCACTTTCTTCTACGCTATTACTTCCATTTGAATCTTTGTTACTCAAAAAAGACTTAATTGTTTCCTTACTCTTTTCATTAATCATCCCTTCCATCTTTATGTGGATGTTTACTTTAAAACTTTTAGGGCTGGTTCAGCTGTAAGAAAAACCACTAACAATTTGTTTTTGTACATTAAACACTTCAGCAATTTGACTTCCTAAACCAGTAAAAGTTTCATTTAAAAATAAACCTTTCTCGCTAATATTCGGAGTAACGTCATCTTTATTTTTATTCGGGCCAAAAGTATTTTTAAAACGAGATGGATCTAAATTATTTTCCAACAATTTTTGATCCATTAAGGTGTACTTCCAGCCTGCTAGTAAGGCGTTAACTGTTTTGTTAGGATCAGAGTTATAGCCATCTTTCAGAATGTTGATAACGTTATTTTTATTCAACTCACCTTTATTTGCTTCAAAGTATTTTTGAGCGCTACTTAAACTCGATATAACCTGGCTGGTAGTGGAAGAACATGCACTTAAAATAATGCCGGAAGCTAAAAGAGGAATTAAATAAAGTTTAAGTTTCATTAGAGTATGGAGTAAAGCACGTAAGGCAAGAGATTTATCGATTGGTCTTGAAATTTTTGATCAGCTAGTTGCTTTACAAAACTACTTTGTTGCAATAATTTAGTCATCACACTATCTTTGCCTATAATTTGTATTCGTAAATTGGTATTAAAGTTGATGGCTCTTGTTAGTTTGTCAAATTTACTTGCAGAAAAGAAACTGGCAGCAGGTGGCATATAGGTGTAAATTATTTCAGCTTCTAATGTACCTGTATCATTTAGATCACCATCTGATAAACCTACTTGACTTAAATAAGTTTTTACAGTATCCTTTTTGTAATTACCTTTAGCATTAATTTTTACTTGAACTCTAAAGTTTTTAGGATCTTTGTATTGAATGCTAAAAGCTTCTACTTTTTGTTTGTCCAGTTTAATTACGTTATTAATTATATTGGCAGTGTCTTGTGTGTATCTTTCCACCAAGCGTAAACCCCTTACTCCAGCACTAGGCTCTACATCTTCAATGCTTCTGCCAGAACCAAAAGATTTACTAAATTTGGAAAAATCTTGTTTTTCTAAAATTTGAGCGTCTAAAAGCGTAAACTTTCAAGCGTCTAATAAAACCCTAGTAGTGCCTTCTGGATTATTTTCAAAAGCTGATTTTAAGGCTGTAACTAAATCAGCTTTATTTTGGGTTAAATTAGTATTTTTACTTGCACTGGTTCTTAATTGATTCAAGCTAGTAAGATTTGCTTCAAAACCAGAATTAGAACAAGCAGTTAAAGCTGTTCCAAACAACATAAATAAAGCGCCTAAGCTTGTCTTATTTCAAATTTTCTTTTTCATATTTTTAGAGTAAGGCATAAAAATAGAAGGGCAATAGATCAAATGATTTACCAGTAAACGAACGACTTTTAATTTTTTTAACATGTTCGTTGTTTTGCATTAGGATATTGAGTTTTTCATGAGTTGTGCTTGCATCAATTTGAAGATTAGCAGGGAAATTAATTGTTCCAGTGATTGGATCAAAGTTACTAAATGCAAATCTTCTTCCATCTGTAGGCGGAGTAAAAGTGAATATGAATGTGCCATCAAAGTTTGCATTTCCATTATTAAAATCATTTTCAGTAACATTGCCATTATTTCCAGAGCTCAAGAAAGATTTAATTAAAGAATTACTTTCTTCACCACTGCCAACAATGCCTTGCATCATAAAAGATATAACTACTTTAAAGTTAGTGGTGCTAGATCAGCTGTAAGAAAATCCAGTTACTTTTTGGCTTTGCACTCCAAGTACTTTAACTATTTGTTGACTTACACCACTGTAAGTTTCATCTAAAAATAAACCTTTCTCACTAATACTTGGAATGACATCATCTTTGCCTTTGTTAGTACCAAATGCTTTTGTAAAGCGACTAGCATCTAGATTTCTTTCCAGTATTTTTTGATCCATTAAGGTGTACTTTCAACCAGCAAAAAGTGAATTAACTGTAGCTTTTGAATCAGTACTGTAACCTTCTTTTAAAAGATTAATTACATTCTTTTTGATTAGTTCACTTTTGTGAGCATCAAAGTATTTTTGCGCACTACTTAAACTAGAAATAACATTGGTGGCAGTTGATGAACAGGCACTTAAAGCAGTGCCGAACAATAAAAATAAAGCATCTAATGGAGTTCTGAATAATAGCCTTTTCTTATTCATCTTTAAAGTAGTGCGTAGTAAAAGAACGGTAACAAGTCAAAGGTTTTACCAGTAAACTTACGGCTTTTAATCTGTTTTACTTGTTCATTGGCTTCCATTAAAGTATTTAACTTTTGGTGAGTAGTGCTTACATCTATTTGTAAGTCTGCTGGAAAGTTAATCTTAGTTGTAAGAGGGTCAAAAGAAGTGGCACCAAATTTTCTACTTTGAGTAGGAGGTGTATAAGTAAAAGTAAAGAAACCATCGAAATTAGCAGAATCACCATTAAAGTCAGTTTCTTTTACTCCGCCATTTTGATTTGATCCATTGTTATCAGAACTTAAAAAAGATTTAATTAATTGTTGACTCTCTGAATCACTTCCAACTTTTCCCTTCATACTAATTTGAATTTGTACTTTAAAGTCAGAGTTACTGGATCACTTGTAATTAAATTGTTTAACAGTTTGACTTTGTACACCTAAGACTTTGGCAATTTGACTACTAAAGCCAGTGTAAGTTTCGTTTAAGTACAATCCCTTTTCACTAACACTAGGAGTTACATCGCCTTTATCGCGTCCGCTACCAAAAGCTTTGATAAAGCGACTATCAACTTCTTTAGAAAGTAATTGCTGGTCCATAAGTGTGTACTTTCAACCAGCTAGCAAAGCATGGACTGCCTTATTAGCATCAGATTCATAACCTTCCTTTAAGATTTTGATGACATTCTTTTTGTTGAGTTCACCTTGACTGGATTCAAAGTATTTTTGAGCACTACTTAAACTAGAAATTACTTGAGTAGCTGCTGAAGAACAAGCGCTTAACAACAAACTAGAACCTAAAAGAGGAATTAATAGAAATTTAAGTTTCAGTTTCATTAGAGGATGGAGTAAAGTACGTAAGGCAGTAAATTAACTGCTTGGTTTGGGTAATTAACACTGGCAAGATTATTAGTGAAGGCACTACTGTTAAGAATCTTTCTCATTACTTCGTCTTTACCAACCATTTCTAATTTCAGATTGGTATTGAAATTAATCGATTTAGTTAACTTGTCAAAATTACTCTTAGAAAACAAATTTGAAGCAGGTGGAGTGTAAGTGAAGATTAAATCAGCTTCTAAAGTGCCTTCTTCCTTATCGCCCAGTTCATTATCTTGAAGACCAATTTGTGACTTATATTTCTCGACTTCACTCTTTTTGTAATTACCTTTTGCATTCAGCTTAATCTGAATAGTGAAATCACGACTACTTTTAAAGCGAATATTAAAAACCTCTACTTTTTGTTGATCCAATAAAATTGCATTTTTAATAAGACTAGCTGTGTTATCACCAAAACGTTCAACAAATCTTAAACCACGTACGCCAGCACTAGGTTCTATGTCTTCCTTACTTTTACCGCTACCAAATGCTTTAACAAATTTAACTGGATCTTGTTTTTCCAAAATCTTCGCATCATAAAGACTAAACTTTCAGGCATCTAGTAAAACCTTAGTGGTACCTTCTGAATTATTTTCAAAGGCAGTTTTTAAAGCATCAATTAAGGTATTTTTGTCCTGAGTTAAATTAATGTCTTTATTGGCTGAAGTTCTCAATTGGTTCAAACTAGTTAAGTTAGCTTGAAAGTCATAGTTAGAACAAGCAGTAAACGCTGTTCCTAATAAAAGAAACAAAGAACCAAAAGCGGTTTTAGATCAAATACTTTTTTTATTCATGCTTCGAAATAAGGTTTAAAAATTAGAGAAGGGCGTAGTAAAAAAAAGGCAAAAGATTAAATGATTTGCCTTTAAAAGAGCGGTTCTTAATCATTCCTACCTGAGTATTAGTTGTCATTAAAGTGTTTAACTTTTCATGACTAGTACTTACCTCAATTTGGAGATCAGCAGGAAAATTGATGGTTCCAGTAATTGGATCAAAGTTGCTGGACGCAAGTCTTCTGCCATCGGAAGGTTGAGTGTAAGTAAAGATAAAATTGCCATCAAAGTTAGCTTGGTCACCTTCAAAATCTTTTTCAGTTACACCACCATTTTGATTATTGCTTCCACTGCCTTTGCTATCAGTACCTAAAAAAGACTTAATTAAAGTTTGACTTGTGCCATCACTACCAACTTTTCCTTTCATTTTGATTAAGATTTTGACCTCAAACTTGGTTTTGCTAGTTCAGCTATAAGAAAAACCAGTTACCTTTTGGCTTTGGACACCAAGAACTTGAGCGATTTGACTACTTACACCGGGATAAGTTTCTGCAAGATATAAACCTTTTTCACTGATGTTCGGGGTAACATCATCTTTAGTGTTAGAACCAAAAGCTTTAGAAAAACGGGAAGGATCCATTGGGTTCTCCAATAACTTTTGATCCAGCAAGCTGTATTTTCAGCCAGCTAATAAAGAATTAACTGTTGACTTGGGATCAGAATTATAACTATCCTTCAAAATGGTTACTAGATTACGTTTATTAAGTTCTTTTTTGTTGGCACTAAAGTACTTCTGGGCACTACTAAAACTGGAAATTACTTCTGTGGTGGCAGAGGAGCAGGCACTTAAAGCTGTGCCAAACAGGAAAAACAAAGAACCTAGACTGGTCTTTGACAAAATCGATTTTTTGTTCATTTTTAAAAAAGTAAATTTTTTAGTTGATTTATTGATTAGTCAGATGGTTTAAAAATACTTGTTTACAAACAATGAGATAAGAGGAGATTTATCCTCTTATTTAGTATGAGTTAACGCAATTCAAAGAAAAGCGAGCGGTATTTAAAAAAACAGAAGTAATCACTAAGTTTGGAATTAATTACCTCCTTTTCAATGACAACTACAATCTCTAACTTAGGCGTTTCTTCTATTACTTCTGGTTGATTGTCTTCTTCTATGCTTGGTTGTTCAATTAAAAGGCCAATAAAATTATGAACTTGGCGTTCAATAAAGAAAACAAAGTTGTTTAAAGAACTAAAATCGTTTTTAATACTTCTTCAAGCTTTAATTAACAGATTAAAGGTATAGTAGTTATTTAATGAGTGGGTGACACGAAAATTAAATAACTTAAATAATGTTTTCGAAAGAAAAACAAGACCGTTAATTAAAAACAAGGAGACTAGTAAGATAGCTAGTACATTTGCTTTAAATTGGCTTTTCATGAAGAAGAAGGTTGGTTAAAATTGAAAGCTTTTTGAACTAAACAAACCCAACAGCTTGACTGAAACTAAATTAGTTCTCGTCTAATTGACCATAATTTTAAAACTTTTAATTGTTGCTTATTCCGCTAATAAGCTCGTTTAACTAGCTCGATTCAAATAAGTAAGATGGCAAAATTAACTAAATTGGCATTAATCAATATTCAACTGTTATTCTAAACTGACTTTTAGCTAAAAAAGTAGATCGATTAAGTTAACTGATTCTTAATAAAAAAGCTTTCATTTTTCAACCAAAAATTATTTTTCAATTGAATGTCTTAAAAAACGAAAAAATCTTATTTCTTGTTCTGTTCGATCATCGAAATGATTTTCAAACTCTTCTCTGTCCGCATTACTTTGACCAATATATTCCCAACTTGATGTTCCATAAAGAATTATTTCTCCGTGTTCCAAATTAAAACTATTGTTGCATTTGGTAGTTTTATCGTGATCTTTTCATAAGAAGTTTTTAAAGATTTAACTAGATTTGTATGGCCTGTTTCTGTTTCAATTTCTTCAGACCAATTTAAATCTTCGTTAGTATTGTTTTAATCAAACTTTATATCTCTGATTTGACATTGGAACAAGCACTTAAAGCTGTATCGCACAAAATAAATAAAGCTCCTAGACTTGTTTTAGATAAAACTTTACTCTTACACATAACGAAGATAGATGAATTAAATGTTGATCCATTTTGCTTTTTTTGGAAAAGACGTGCTTGAAAACTTCAAAATCACTTAATTTTTAGGTTTCCTGGTTTGTAATTTTGAAGTAAAAAGCTTTTAAAAATTTTTAAAAAAGTTTTTTATTTAACTAAAAAGTTTTGAGGAATTAAGTAAATCTTAATAAGTTATCTAATTACGTAAACTAAATAACTAAAAAGGTTGATTTTGTAGTCATCAATTTGAAAAGCTCTTGCTCTACCAAAAACACTTAATTCAAAAAGTTTTTTGCTAACTTCGTCTTTTCCTTCTAATCTCCAGCTAAATGAAACATAAAAGTCGATTGCTTCTGAATCAGGTTTAAAGTTTTCTGCTGCAAATGGATGGTTGGGAGAAGCTTTAAAAGTAAAAAAGAGATCAAAATAAAGCTTTTCACCTTTTTTTGGAGCGAAACCTATTGAAAAATCATTGCCATACCATGTAAAGGTATAGTTCAATTTGGCAACAACTGAAAAATCACTAAGGCTTTGGTAATTCAAATTGAAATACTCAACCTTTTGTTCGATACTAAAGAGACTAGTGCTTAAGGAAATTTTCAATTAGTTCAATTAGTTTCAGAACTCTCTATTTGACCGTTTTCTACACTTTGCTTCCAGGCATTCAAGAGTACACTAGCTGTTTTGCTAGGGTTAGTTTCGTAAGACTTCTTTAAAGAACTTATTAAATTCTTCTTTCCCTCAGAAGTACTAACTGATTCAGACAAATCAAGGTCTTGATCGGTGGTTTCCTTAATAAGACTTTTCAAATTAGCTCCAACATTGACGCTAGAACAAGCGCTAAATAATAAAGCTGAAAATAATAACGGAGTTGATAGATATTTAAATTTCATTAGAGGACTGAATAAACCAGATATAGCAGTAAATTTACTTTGTAATCAAAGTAAATTTTATCTACCAATTTTCCAATAGAACCATTATCTAAAAGTTTTTTGGTTATTTCATCCTTAGGAAAAACTTCTAGTCTCATTGTTGTTTAAAAATCAATGGAATATCACAAATTCCCTAATTTATTATCAGAGTTTTTAACAATACTTGTCTCTAGAATTGGTTTGTTTTTAAATGGTTTGTATGTGTAAATAAGATCAAAATTAAGCAAGTAGTTGCCAAAGCCCGACATTATTAACCCTCCTTGATTTGGTTTTTGATTAATATCAGCATTAAAAGTTGTGTTCATTTTAACAACAACCTTAAAATCATTTTTATTGTTTGCCTGAATGTCAAACAATTCAACTTTTTCGCTAATTGTTATTGATTTCTTGTGGAATTAAATGAATGGATTGATAAAAAAGGTTAAACATTACTTCTACCTTCTTCTCTCATAAGACTATTAAATCATTTGGAATAATACCAGTACCACAGGTGCTAAACAATAAAACTGCTGATAAAAATGGAACTGAAAGGTACTTAAATTTAATTCCTTAATTCTGCTTTTTTTGCAAAAAGACCTTTTGACACCTTAAGCAAAGAATAAAAGTTTTATTCTTAAGATAAGGACTAAACCAAAAATGAGAAAGAAATTAGCAAAAGTAAAAGTTCACATTCAGTCTTTGGATTCATTGCTTTCATCAATGACGATGCCGATTATTGGCATCTTTATTGCTTGGGGACTTTTAGCTTCTTTTTTTATTCCATCTGGTTGAACACCTGATAAAAACCTAGCTTTAATGGTGGAATAGGAATCCAATATGTGATTCCGACCATAATTGTTTTTTTGGTGGTAAAAAGATTTTGAAATACGTGGTGGTGTGATTGCTGTCATTATTGCCATTGCTGTAATAGCTGCTGGTCAAACCGAAGCATTTACAAAAATTGTTGGTCAAAAATCAGTAATGTTTTTATGTGTGATGATATTCGTCCCAATTGCAGCGCTAATCTTAAAACACACTGAAAAGTTTTGAATTCACCGTATTAAAAGTGGCTTTGAAATGCTGGTAAATAATTTTTACCTTGGCTTTTTAGGTTTCGCCTTAATATTTCCTAGTTTTTACCTATCAATTTACTTAATTGGTTACATTCAATTAGGTTTAAAACTGCTTGTTGAAATAATGCAACAGTACAAACTTTATCCAATAGCTGCCATTGTGATAGAGCCGGCCAAAGTTTTATTCCTAAATAATGCCATTAACCATGGCGTGCTAACTCCATTAGGCTTACAACAAGTAAGAGACAGTGGTAAATCAATTCTTTTCTTATTGGAATCAAATCCTGGTCCAGGTTTGGGTCTCCTTGTTGCTTTCTTAATCTTTTTCTTTAAACGTGATAAAAAGCTTTCTAGTAATGCGGCATCTTCTGGTCCTATTCACCTCTTTGGTGGTATCCATGAAGTTTACTTTCCGTTTGTGCTATTAAAACCTGTGTTAATTCTGGCCACAATTGCTGGTGGTGTTGTGGGAAACGGCATTTTGCAAATATTTAATGCTGGGACAATTGCACCAGTGTCACCTGGTTCTGTTATTGCTGGTTTTCTCCAAATTAATAAAACACCTTTAGATGTAGCTGGTTATGCTTTGGCACTCGTTTTATCAGCTGTAACTTCGTTGTTAATTTCGCTTTTATTACTTTCTTTAACACGTAAAAAACAATTGAAAACACTGCAAGAAGCGCAAGCACAAGTTGCAGAAATGAAGCAAACGCCTGCAAAAAAACCAAGACAAAAAGATACTCCAGCAATTGCTACAAAGATAGACTTTTCCCAAGTTACCTTTGTTTGCGATGCAGGTATGGGTTCTTCTACTATGGGAGCAGCTATATTTAGAAAAGAACTAAAAAATCAAAATATTGAAGACATTACAGTGATTAATAAAGCAATAGTTGATTTAAAAGATGAAAAGGTAATTATTACCATTTCCCAACTTTATGATCGCGTAAAAGCGAAGCGTGCAGACGCTACGATTTATACCATCAATCAGTTCTTAGATAAACAAGGCTATTTAACTATTATTGAAAAGATTAAAAATGAAAAGAATTAATGTATTGCACTTTGGCGCTGGTAATATAGGTAGAGGTGTTATATTACCGATTTACCAACAAAATGACTTTTCCATTGATTTAGTGGAATTGAATCAAAATACAGTTAATGAGTTACAAAAGCAAAAACAATACCAAGTTCATTACCTTGATTGTGATCAAAGTCAGTTAGTTAATGACTTTAATACTTGAAACTTAAAGGATGAAGCGAAGATTATTGAACTAATGGAAAGAGCTGATGTTATTTCCACTTCAATAGGTGCTAAAAACTTAGCTAGTTTAAAAACTTTGTTTGACAAAGCTAAGTTCCATAAAAGGGCTATAGTGCTTTGCTTTGAAAACGGCTTTCGGATTAGTTCTAACTTTAAAAACATTTTGCAATTAAATAACACACAAGTAAATTTTGTGGATGTGGTAATCGATACAATTGCACCTAACTTCGAAAAGAAAGCGAATTTCTTGGATATTTATTGCGAGAAGTATTCAGAAATTTATGCTGAAACTTTCCCGCTAGAAATTAAGGGAGTAAATCAAACGAATAGTTTAGATAGATTCATTATTAAAAAACTTTTATTAGTCAATGCACTGCACAGTGTTATTGGACTTTTAGGCTTTCAACAAAAGCTTAAGTATGTACATGAAACATTGCAAGTTAAAAGTAATTTGACTTTTGTAGAAAAATTAGCCCAACAAATTATTGATGCCCTTTGTGCTGAATATCCTGAATTTAATAAAAATAATTTGTTAAGTTATGGCAAAAATAACTTAGTCCGTTTTGCTAATCCCAAGATTCAAGATTTAAACACACGCTTAATTAGGGAACCATTACGTAAACTTAACCAAAATGAACGCTTTTATGCCATTTATAAACTCTTTAAAAAGAATAAGATTGCACTAAATAATATTTTGCAAGTTTATCTGATGGTTTTAAAAACTAATATAACAGATGATACCGAAAGCCAACAAATTGCTAAATTAATAAATGAAAAAGCTTGAACAGAGTTAGCGAAATTATCCAGTTTAGAAGAGTCAGAATGAAACCTAATTAAACAAGAATTAAGCCGTGAAATTACTAAAAAATAACATTTACATTAACGTTTATTTAAAAAACAAACAAGAAATTTTTGAATTTGTTTTTAAAAAGTTTAAAGAAGATGGTGCTGTACTAGATAGTTTTTTACCAGCAATTGTGGAACGCGATAAAGCAGCTAGTGTAGCAATTGGTAATTACCTCTTTTTACCCCACCCAGTTTATGACGAAATAGCAAACATTCAAAAAGAAAAAATGGTGTTTATTGGTTTAAAAGATGTAATTAACATTGATGGTCAACCAATTAAATTTATCTGTGGTTTAGCCTTAAAAGGTGAGCACCAAATGGATGCGTTGCAATCTTTAGCAATAGCTTTTTCAGACCCTGAAGAAGTGGAAAAGTTAGTTAAAGATAAGGATTTAACACAAGATAAGGTACTTGAATTTTTAGCAAAACACAACTAAGCAGTCACAATATTACTTAATTAAAAATAGTAAGTAACTAAATAAATTAACTTGCTTTTCGTCAGTTTTGTAACTAATATAGTCATTTAAAAAATTTTATCCAGATTTTTTTGGCTAAGTTCATCTTGTCCTTTTAATGATCAGCTAAGTGAAACTTTAAGCTAAATTCATTCTGTTACTATTTTTTCAGCTTTTAATTCTTCATTTTTAACGATGAAGTTTTCTGCTGTAAATTGTTTTTTTGATTAGGTTTAATAGTAAGGGAAAGATCGAAAGAATACTCTTATCACCATTCTTGGCAGTGAAGCCATTATTGGAATACTGTCCATGCCAATTAAAGGTATAGATCAACTTAGCACTAATTGAAAAATTTTAATACTTCGATATTCAATGTCGAAACGCTCTACCTTTCGCTCCATTTTTAAAAGGATCTTTTTCTGTTGGATTAGTTACTTGGTCAAAATCAAGATCCGCAATGCCTAATTTGCCCTCTTCTGAGCTTTGTTTTCATGCGTCTAACAATAGCTTTGTTGTATCTTTTGGATTAACTTCATAAGATTTCTTTAAAGAATTTATGAGGTTTTGCCTACCTTCCGGAATTTTAATTGCTTTAGACAAATCAAAATCTTTGCCAGTTGTTTCTTTAAGAAGATTCCTAAGATTGGCTTGGATCCATGTGCAAGCACTTAATATTGTTCCACATAACAAAAACAAAGAACCGAAGGAAATCCTTGACAAAAGTTTCTTTTTACGCATTAACATAAAAAATAATGCATGATTTTGCTTTTTTGGAAAAGCCTTTTTAAGAAGAGGAAATTAATTGAAATTTCTGTTTAAACGTTATTTAAAAAATGAACTTTTTAAATTAGAAAAGCTATCTTTGTTGGTATTTTTATTCATCTAAAAAATACTCAAAAACTTTTTGCAAGTTTTGTTAGCACTTTTTCTCTACTCATGCAATTCATTAGTTAGAAGGGTGACAAGTGGTTTTTTTAGCTTGATCAGTGTGTTGCCTAACTTTATTTACTTAAATTACCAATGAGCAACAATAATAAATATTTCACTATAACTAAAAAACAGTACAAAAAAATGCGCCGCAATAAAATTGAACTTCTTTTTAATGTGAAAGTCCTAAAAAAGAAAAACGGTCGGCAAAAGTTCAAAATACTGCATGAAGTTGAGAATAAGCCAAAAATACCAATTAAAATAATTGAAGATCAACCGGAGTCGCCAAAGCCACCTAAAACTCCTAAACCCCCTAAACCGCCTAAAGGTCCTGACAATCCTGAGGAGCCGGATTCTCCAGAAGAACCCAAAGAAACAGATCAACCAGGTGGTCCCGATAATCCTAATGCAGGAAATAAAAAGATGCCAACACCTGAAGAATACGTAACTCGTAAAGAATTCAACGAATTCAAAGATTCAAACAATCAACGACTTACAAAGATTGAAAATAAGGTTGATAAGTTAGAAGTCAAAGTCGACAAATTAGCTGAAATTGTTCAAACTCAGGGCGAAGAAATCAAAGAACTGAAAGTGGAGCAAAAAGCCCAGGGTGAAACCCTACAGCTAATTTTGAAAACACTGCAAAAGATGAACGATCGTCTGGACAGAATGGAAACTCGCTTAGACAAGATCGATTCGCCCAAATAGCTTTAGGCTAAAAAGAAGATACCAATGGCTAAAACGCTGTTGGCATTTTTATTCATCTAAAACTGCCCAAAAAAATTTTTTGCAATTTTGTTGGCACTTTTTAATTGCTATTTCAATTTATTTAGTGGAGGAGCAAAATGCAATTTTTTTGATTTTCTAGAATCCTCTAGAAAGGAGGTGTTAATTATGAAATATAAAAAGATAGGTGATATTAAATCTAAAGGATTTTTCGAATCTAAAGAAGAATATAAGGAACTTTTAAAAAAATGTGAACTAATTTCTTTTGAAGCTTTTTCTGAAAAATTACCTAATGGTGATTATGAAGTGACTAAGAAACTTAAGCTTGTAGAAAAAGGTCCAAATTGAAAAAGAAAAACATACAAACAATCTTTAGAGAATGAAAAAATCTTAAAGTGGCTTATAGAGCACGACTAACAATAAAAGCAAGCCAAAATCGGCTTGCTTTTATTTTGTCTAATCTAACTCAAAACTCAGTTTACCGTACTTCCCGTTTATCAGTTCACTAACAAACAGTTCGCACGCTAAATTAATGTTGAGCTGATTCGATTTTTGCAACAACTTACGACTGATAGCAAATTTATGTAAAAACTCAGTGAAGTCTGTACCTTCATATGGTAACAATTGCGGATAATGTTTGCTTAAATAACAAAAAGCAAACGCACCTACATCTTCCAAAGGGACTACTTCCCTTTTAATAACGTTAGTTAAAACTAACTTGTAACCAACAGCCATTTCATCAATTCGTTTAAAGAAGACTCCTGGGGTATCGCTCAAATAAAACTCCGAACTTATTTGGTTTCAACTCATTGACTTGGTAACCCCAGCCCGATTAGCTACCTGTAAGTGATTTTTATTTAGTAACAAGTTAATTAAACTGGATTTACCAACGTTGGGCATTCCAATAACCGCTAACCGAAACTGGTGCACTAATAAACCTTTGGCTTTTAACTGATTCTTTTTTGCTTGAAACAAAGTTTGCAGTTTCTTAATAACTAAGCGTTTAAGTTGCAAACCTTGCTTTAAGGTTCCCCATAAAATATTGGAGTTAGCCACTGTTTGTGCTAAATCAGCCTTTAAAGCTAAAGTGAGTTTCGGTTTATTGGTAAAGTAAGCCGTAATTTCGGGGTTTTGGGTTAACGTTGGTGCACGCGCATCAACTACCTCAATTACACCATCTAAACTACTAGCTAGTTTTTTGAGTTGGTCATGGGTCTTCTTCATGTGACCCGGAAACCAGTTAATCTTGACTGCGGAGGTAAATGTATCCATCGATCCCTCGGATCCTTGTAATTGATACTAAACAATCTTTGTCAATTTTCCGGATGATGCGGATGAGGTTAGGAATTTCCACATACATAGTTACTGAAACTATCATCCGTTTTTTGGCTAATGAGTATCCACCCATCGTTTCTTGAATGGACAGACTGTGAGTCGCTTTATCGTTGAGCAGAGCCAACCTCACTTCTTCAATCTTGTCAGTAAAGACCTTAATTTCAGCAAAGTTATACCTTGGGAAAAGGTGACTCACTACTGTACCCGTAAATAACACCGAAAAGAAAGTGGCGATTAAGTTGGGTGAAAAGAAAAGGTTAACCTGTCAAGCACTTTTTTTGTAATCGGGAATATCTTGGAGCACAATACTGCCAGCGACAAACGAACCCATTAAGATCGCAATGATCAAAATAAAGGTATTGACATAAAAGAGAATTGGCCCCACGGAGCGGTTTTTCTTTCTCGCAAAGTATTGCGTTAGGAAATCGGCACCCCCAGCACAAGATCCCAAGATGTAGAGGATTGCTAAACTCGTACCGTTAACAAAACCATAGATCCCGGCATAAATAAAGGTCGAAATAATGACATTGCCTTGTGAAGTATCATTTCATAAGAACGGTACAAATAACGCGCTTTGATCAACACCTTCAGCTTTTTTTGCAGCTTCCCAAAATTCAGTGTGGTGCACTGCGGACAACATCGATGGCAATTGATTAGCCCCCGGAATAATACTAAAGATAAAACCAAAGACGTTGGAAGCGACCACGTAATGGGTTGACAGAATGGTGAAACGTTTCCCAATTTTTTTGTAAGAAAAAATAATCAGGGGAATGTTGACTATTACATAAAACAGCCAGTAAAAGATGTTGAACACTAGGTTGCGTTGTCCATCTTCGAGGTTCTTCAACAAGACAAACAGTAAACGCGCAAAACCTTGGGTTAGCGAACTAATCCCAAAGCTGTACAATCCCGTTTGTTGGATAAACACCAGCAAAAAGAAGGCGTTCACTAAAGCCAGGATGTAGACAATTGTGTACTTTAAAGGTTTTTTAGCCTCATAAAGGGCACTAAACATCAACAGCGAACTAGTAATCTTGGCCCTTTTAGAAGCTGAAGTAGTTTCAGTTTTGTCTTGATCCTTCCTTGTGAATCACCTTTTCAGTCAATCCATGGTGAAAAAGTAAAACTATTGTTTTCGCTCTTTAATTTTGGCAGCTTTACCAGAGCGCTCACGCATATATGAAATGTAAGCGCGTCTTACTTTACCCCGTCTTTTCAATTCAATTGAAATATTAGGGTTATGAATTTGGAAGTTTTTTTCAATCGGAATACCATCAGTGGTTTTACGCACAATGAAGGTTTCACTAATACCTTTACCACGACGGCGTAGGACAGTTCCCGTAAAGTTTTGGATTCTGACCTTTTCCTTTTCTTTAAGTTTGATGGCAACATTCACTTCGTCACCAGCGCTAAACTCAGGCACATAAGCCTTTAATTGCTTTTGTTCTACTAAATCTATTAAAGCTTGTTTATTTATTTTTTTCATCTTTCTTTGCTAAATATTGCTTATACAAATCAGGACGGTATTGAGCCGTTCTGAGCAGTTGTTGTTCTTTGCGATAAGCCTCTATCTTTTTATGGTCACCTGATAATAATATTTCAGGTACCTTATCCCCGTCTAGATCATATGGCCTAGTGTAAGCGGGGAAATCCAGTAAGTAGTTGTTAAATGATTCGTGGATTAAGCTCTGATCATTAATGACACCCTTAATTAAACGAACAGTGGCATCAATCACACTTAAAGCCACTAACTCCCCACCACTCAACACAAAGTCACCTAACGAAATAATTTGATCGACATGTTTATACAAGCGGTAATCAAAGCCCTCATAGTGACCACAGATCAAAATGAGGTGTTCCGTTTGGGTTAAAGCTTGCGCCATGGGTTGGGAAAACTGTTGTCCCTCAGGTGATAATAAAACTACTTTCGATTGTGGAGTTCTACAAGCCTTTAAAGCTTTGAGAATTGGTTCAGCTTTTAGAATCATCCCGCCACCTCCTCCGTAAGCCATATCATCAACCGTTTGGTGTTTGTCCTTGCAATACTGCCTTCAGTTAATTACTTCAAACTCAACGAGCTTTGCTTTTTGAGCTTGGGCCATAATCGAAGCGTTTAAGTAAGGTCAAACTACCTGTTCAAAGAGGGTTAAAACAGTGATTTTCACACTTACTTGGACTTAGCTACTGGCTTTTTCTTACTTTCTACAAACTTTTTCCAAAGACCAGTCTGACTAAACAAAGAGCGAACTGTATCAGTTGGTTTTGCTCCCTTATTGAGTCATTCCAAGGCCACAGCTTCATCAATCTTGCACTTATTTTCTTTTAATGCAGGATTGAGATGGCCAATTAACGCAATGTACTTACCATCACGTTTAACTCTGGAATCAACAGCTACTATTCTGTAGGTTGGGTAGTGAACACGACCCATGCGCATTAATCTTATTTTTACCAATCAAGTCTCCTTTTAAAATAAAGCGGATAATTATACATTGCCGTTAGGTAGTTTTACCTAACTCTAAGAATAGCGCTCAATGAACTGGACAATCCGACTCTTATGTTTTTGAGCGAAGTATTTATCCATAAAGAAGTGGATGTGTACTTGGCCAATCAAAACGTTAATCAATCCAGCCAGAGCAGCGTTAGCCGTTAATAACCAGATAAAGATTTCAATGTTTTTTGTTTGTAAGGTAATTGCTTGAAAGATAAACAACAAGGGTACAAAAACAATAACCCCTTGCATTAAGGAAACTATTCACGAATACAACACCCGGTTGGTATTTTGGAAGTACTGTTGACTGGTAGCGCCAATGGCCACAAAGAACACTTGCGCTTGCACAATTAAGGAAAACTGATTAGCGAGCATTAAGGTAGCTGCACTTGTATCAAACAGGTGCACTAGGATTTCTTTGCCCAATCCAACTGCCAGAATTAAATACAGCAAAGCAGCAAATACCAAACACACTAACAACGTATAAACATTAATCCTTTTAATGTCAGCCAACTGGCCCTGACCAAACTTGTAGGATACCACTGTCCGCACCCCTTGGAGAACACCAAAGATGGCGGCTGAAGTTAGGTTAGCAATTGCAATGGGTCCCGTTAGTAACACCAAGTAAAAGGTATCACTTTGATCCGTAAGCGTTTTGGTCAAATTCACCAAAAAGCTTTCGTAAAAGGTGTTGAGAATTGAGAGCGAACCATTACGGAAAAAGGAGGCTAACCCCACCATTGAAATGACCAAAAAGAGGTTAAAACTAACCCGTTTAAAGGAAAAGTCCCTCAACGAAAGGTAGGTTAAACCACGTTTATTTAAACTAATAAGGTACACCACATAAGCCATAAAGGTAATGAAGTAAACCAAAATCGCAGCCACCGCGGAGCCCACAACCCCTAAAGTGGAAAAACGCACTAACAAGAAAACAAACAGGACGTTCAGTAAGTTCGATAAAGGAGGCACAATTGCAATAAACAGTTGCCGGCCCTCTGATTGGCCTAAGTAAAAAAACAAGCGGCTCAACATCGGTATGATGTTAAAGCCAATCAAAATGTAAACGTACTCTGAACCAATCGCAACCGCCTTTTCGTTAAAGAAGTGTTGAAACTGATCCGCTACTTGCACGTGGTGTTGTTCATCGACTTTATCCAAGTTAAACTGCAACCACTCTTTGGCAATCGCTAGCACAATAATTTGGGTCACTAGGGCAAATAAGGTAGTGGAAACTAACCCGGTATTCCAAGCATCCTTAATCTTTTTCTCATCACCCTTACCAATGGTTTTGGAAAAGATCACCCCCGTACCCAACGGCACAAAGATGGTAATGGCGTTGAGAATGACTACAATTGGCTGGCTAATATTTAAGACTGTTTTGACTAATTGACTAGCTGTTAGTTCCGTGTGGTTGGGAATGTCCCCACCCTTATAAAACGCGGATGCTTTAAATTCCTCAATTAAGGCCTGGTCAGTAAAGATTGAATCGGGATTAAGTGGTCCATGCGGGACAAACTTAATCACCATAATTTGGTCCACAAAGACATAAGCAGCGGAAAACAGCGCAAAGAAAAAAGTCGGAATAGCAAAGCGCAAGATCGTTAGAAACAGTCTTTTCGATTCAAAGGATTTTTGGATGAGGACTTGCTTCGCTAGACGATCTTGTTGGCTATTCACTTAGTTAGATTCCTTAAATAATTTGAGGTATTCACCATACCCCGCCGATTCCATTTCCGCAAACGGAATGAACTTTAGAGCAGCGGAGTTAATGCAGTAACGCAAACCACCATGCTCTTCGGGACCATCACGAAAGACGTGCCCTAGGTGGCTGTTGGAGTTTTTGGCGCGTACTTCGGTGCGGATCATCCCGTGTGATTCATCGCGGTAGTTAGCAATTAAGTCTTGACTAATTGGTTTGGTAAACGCTGGTCAACCACAACCGGATTTGAACTTATCAGTTGAGATAAACAACGGTTCACCGGACGTAATGTCAACGTAAATACCCTTTTCGTAATGGCGGTTATATTCATTAGTATAGGGCGGTTCGGTGTGGGCGTTTTGGGTAACGTCGTACTGTAGTTTAGTTAAGGTCCGCTTTAGTTCCCCGTCACTCTTTTTTTGGTACTTACTCATGCACTGAATTTTTCACTATTATTTAAGTAAACATCAAGGAAACTTTTGCCTATTTTGATGTGCGACTTAATTTCAATTATACCCTGATTTTTAGGTTTTAAATGTAGCTCTTTTTCCGAACAGAGCATTCCCATGGAATCATACCCCGCAATTTTAGCCTTCTTGATAATCGTGCCATCAGGTAAAACACCACCAACATGGACTAAAACAGTTTTAAGGCCAACCCGAACATTATCCGCCCCACACACGACATCCAAGGACTTATTGGAACCAGTGTTGACCTTACACCTTTTCAAATGAGTGTTGGGAATCGGAATGGCCGAAATGACCTCACAAACCACAAAGGGTACCTTTTTGGCTAAGGGTGTTAAATCATAGTTCAACAGCTCACTAATCTTTTTGACCAACTTCAAAGAAGGGTAATTCAACCCTGCGGTAAAGTGATGGGAAATTAATCCCCTGCGAAAGCTCTTTTTAATGTCAAAGAAGTTAAACCCAGCAATCTCATTGGCTTCATTGACAAAGAAGCTAAAGTTCTGGTTCTTTTCTTGGCGCAAAGTAGCTTGCGTGCGGCTACCAATTAAACCAAACATACAGTTCTTAAGTGTTTTCTTGGGACAAAAAATGGTGATAAAGTCCTGAGAAATATCAAAAAAACCGTAACGCATTGTTAACTATTCGCCTTGCAGCACAATTACATAGCTGTTCAATCCCGTGTGGGTACAAATGACCGGGGACAGTAAGCTCTGTTCTATATTAATGGAATGATCCAACAGTTTATCAAACTCTTGGACTAAATTAGTGTTGGTCTGTTCGTCAAAGACGGTGGTAAGAAAGGCACCCCGTTTCAGTTTAGCGGAGTGTTTTAACAGCTGTTTCTTTAAAAACTTGACTGCTAGCTTATGCGCATCATGAGTCGAACGCGCTTTATCAAAGAACTGTAACTTACCATTTTCACCTAAAAAACTAATCAGTAAGTGAAACTTTAAGCTCTTGATAATAAAGGATTTAAGATTGGAAAGCCGCCCGCCTGCCACTAGTGGTTTGGTATCAGTGACAAACAACACTGCGGCCGTTTTCTTGCGGTACTGCTCCACAAAGCGATCCAAGCCTTCCCTGGAATAAGGATTGGTATTCAACCAACCCTTAATGTCATTGATTAGCCAATCAATGATCATTTCCACCGCTCTGGCATCCAATACATGGAACTTCGAAGCGTAATCCTTTTCAACTAACTTCCAGTTCGCGTACGAAGCGGAGATTTCCTTATCAATCGGAATGCCGATAATCAGGTCATACTGCTCCACTAGTTCACTCACCTTAGCGCGCACTATTTCTTCAGTAGTTTGCGATGTGGAGAACTTCACTCCCTTCGGAGCGTTAGCCATCTTGTCCGTGAGGGTTTTTAAATCAATGTCAAACCCCGAACTAAAGGTGGTTTCAGCTTCAGGAGTTGATTCAATGATGAAAAGCGGTAGCACATACAGATGTTGTGCCTGGCTTTCCTTGAGGTTACTAACAGAGTCAACTAAGAATGCGACTCTCACTATTCAAGCACTTCCGTGACTGAACCAGCACCAACCGTTCGACCACCTTCACGGATGGAGAACTTACTACCTTTTTCACAAGCAATTGGTGCAATTAGTTCAACTGTAATCGAGGTATTGTCACCTGGTAGCACCATTTCGGTGTTTTCTGGTAGGGAAATCGAACCAGTAACGTCTGTAGTACGGAAGTAGAATTGGGGACGGTAACCGTTTAAGAAACCGGTGTGACGACCACCTTCTTCCTTCTTTAAAGCATAGATTTCCGCTTTAAATTTCTTGTGCGGTTTAATCGAACCTGGTTTAGCTAACACTTGACCACGTTCCACTTCTTTACGGTCCACACCACGGAGTAATACCCCAGCGTTGTCCCCAGCCATTGCTGAATCAAGTTCCTTTTTGAACATTTCGATTCCGGTAACAACTGCTTTACGGATTGGACGTAAACCAACGATTTCAATTTCTTGACCTACTTTCAATTCACCACGTTCAACCCGACCGGTAACCACGGTACCACGGCCAGTAATCGTCATGGTGTCTTCGATTGCCAACAAGAAGGGTTTGTCCACTTCACGTTCAGGAGTTGGAATCCATTCATCAACTGCATTCATTAAATCATGGATCTTAGCTTCCCACTTAGGATCACCTTCAAGCGCTTTAAGTGCAGAACCATAAATAATAGGGGTGTTCTTGCCATCAAAGCCGTAAGAAGTTAATAAGTCACGTACCTCTTCTGCTACTAACTCTTGCACTTCTTCATCAGTTGCAATGTCACACTTGTTTAGGAACACTACCATGCGTGGCACACCCACTTGGCGGGCCAACAAAATGTGTTCACGGGTTTGGGGCATAACACTGTCAGTTGCTGAAACTACTAGAATGGCACCATCCATTTGTGCAGCACCAGTAATCATGTTCTTAATGTAGTCAGCGTGTCCTGGACAGTCAACGTGAGCATAGTGACGCTTGTCAGAGGAGTACTCCACGTGAGCGGAGTTAATCGTAATTCCCCGTGCTTTTTCTTCCGGAGCCTTATCGATTTGGTCGTAACGAGTAGCAGCTGATTTACCTTCTTTTGCTAATACAGTACAAATAGCTGCTGTTAAAGTTGTTTTACCGTGGTCAATGTGGCCAATAGTACCTACATTAACGTGGGGTTTAGATCGGTCAAATTTCTCTCTTGCCATGTGTTTGAATTACGTCTCTAATTTTACATAAGTTTGAGATAAAGCTGTAGAGAGGCTTATAATTCTTGGAGTAGTGTAATTCATATGGAGCAAAATAATTGCAAAATCAGAACTTGATTTGCCAAACTGGCGTTAGCACAAAAGGTCTTCTTGGGGGTAATTCCCTTATTTTTTATTTGTTTTGTTTTTGTAATTGCTGACATTGTCATCAGTTTGCAAAATAAAGGCCACATTATAGAAGAGATAGATAAGTTTACTAACCAGAGCAATGTTATGTTGCTCATTTATGCATGTTGGTATGTAAGCAAACCAAAGAGCCATTATCTCAAGAACCAGCAGTTCTTTTTGAGTGCCTTTGCTTACATCATCTTTACTTTTTTAGGCTACAACGTCATTTTGGCGGCTTCACAACAAGCTTACAGTGATAAGGATGCTTATTCCTTGGCAAGTTCTGTCTTTCTCCATGTGTTGGCACCAATTGCTTTCTTGGTTGCAGGTATAGTTAAGATGAAAACGGACAAGGATGTAACCTTTAATCATTTTTGAAAAAGTTTGGGTTACTTCATGATCTATCCCTTAGTGTATGGGTTGTACTTAGCGACCATTCCTTATGTAAGGGGTCATTATGTGAGTGATGATGGTAAGTCTACTACCTATGTGGTTTATGGGGAGATTACCAACACCAAGGATAACCCGATCGTCGCTTGACCAGTAGTAATTTGTTTCCTTTTCATTTACTTTCCCTTGAGCTTCTTAGCGGTTTATGCCTTGCAATGTAAGTTATTGAATAGACCTCTAAAAGCACAATTTAAGTGTGCAACCAATAAATGCCCAAAATAAGAAAAGCCGTTATTCCCGCAGCCGGACTGGGTACCAGATTACTCCCAGCCACTAAGGCCATTCCCAAAGAGATGTTGCCCTTAGTCAATAAACCCACAATTCAGTACATTGTGGAAGAAGCAGTAGCGAGTGGCATTAAGGAAATCCTAGTAATTGTGTCTTCTAAAAAGGAAGCGATTATTGACCACTTTGACTATGACTTTATCTTGGAAAATGCTTTACTCCAAAAGCACAAAGATCAAGAACACCAAGAAATTAAGGATATTGCCAACTTAGCGCACATTTACTTTGTCCGCCAAAAACACCAACACGGTTTAGGAGATGCCATTTTGCACGCCAAGTCGTTTGTTGGTAACGAAGACTTTGCCGTCCTTTTAGGTGATGATGTCGTGTTTGGTGAACAACCGGCATTAGCACAATGTATTCAAGCTTATGAACAAACAGATTGTCAGGTAATAGGCGTGCAAGAAGTGCCCCATGACCAGGTCAACAAGTATGGCATAGTAACCCCTGAAGCTAATTGACAAAAACAAGCGTTAGTCAAAATTTTGGGCATGGTGGAAAAACCAGCTGTCAACGAAGCCAAGAGCAACCTAGCCATTTTGAGCCGTTACATCCTTAAACCCAGTATTTTTACAGCTCTTAAACAAGTACCGTTTGGTGTTGGAGGTGAGTTACAGTTGACTGATGGGTTGAACTATTGTTTACAGCAAGGGGAACCCTTCTTTGCCAAGCACTTTGGTGGTACCCGGTTTGATGTCGGTACTAAAAATGGTTTTATTAAAGCGAACCTGTACACGGCTTTAAAAACCGATGCCATTACTAAGGATGAAGTCTTGGCCATCCTCAAGGAATTTGTTTAAACAATTTGCTTAAAGCTTGGCACCGTTCAAGGTTAAACCCAAGAAGTTTTCTGGTTTAATTAAACGGCTAAACGGACACATGGCATGCGCTGCCGTAATGACTGTTTGCGCTGTGGTTTGGTCCACGCCCGTAATCGCTAATTCAACACCGGCTTTAATGTGGAACAGACCGTCCTGGTCATGCAGTTCGACACTAACACCCACAATAGGTTGAGTAGCTAATTGCAGTTGGTGCTGTTGTAAAACCACACGCACAGCTTGCGAAAAACAACCAGCATAAGCTGAAGCAAACAGCTGTTCGGGGTTGTTTTTGTCCTGGTGAGTAGCACCAGGTTTAGGAAATGCTAAACTAACGGTAAAACCATCAACGGTTTGGACAACGCCTTCCCTGCCGGCGCTAGCGTGTGCTGTTGTTTTGTAAATTACTGCCATTGTTACTGATATTATATTTAGAAAGATGACAAAAGATCTCCTAGCACTTTTAAAAGAACGGGGCTTATTTGTACAAGCTAACTTTGAAAAGGAATTAAAGCAATTATTAAACCAAGGATCATTTGCTTTCTATGTTGGCTTTGACCCCACAGCACCCTCACTCCATATTGGCAACTATGTGCTGCTCCATGTAGCACAAATCTTCCAAGCAATGGGTCATATTCCCCACGTCTTGTTGGGTAGTGGGACGGCTTTAATAGGTGATCCTACTGGCAGAATGGAACTGCGCCAGATGATGAGTCGTGAAACAATTGCCGAAAATACGCGCAACATTAAAAAACAAATTAGGCGCTTTTTGGGTAGTAATGTAGTGTTTTGCCAAAACGAGACCTGGTTAAAGAAACTCAATTACATTGAGGTCATCAGGGAATTGGGACCCTGCTTTTCGGTCAACAAGATGTTAGCGACCGATGCCTTTAGTGCTCGTTGGGAACGCGGTTTAACGTTGATGGAATTGAACTACATGGTATTACAGGCCTATGACTTCTATTATTTAAACCAAAAGTATGGGGTGCAACTGCAAATAGGGGGCAGTGACCAGTGGGCTAATATCCTAGCGGGGGCTGATTTAATTAGACGCAAAACCCAAAAACAGGTGTACGGCATGACCACTAACTTATTAGTGAAAGCTAATGGGGAAAAGATGGGTAAGAGTGCTAGTGGAGCTTTATGGTTAGATCCACAAAAGACCAGTCCGTATGACTTCTACCAGTATTGGATTAACCTTGATGATGCTAGTTTGCAAAAGGTTTTCCTTATGCTCACTAAATTAGATACCAAGGCAATTGAAACCCTCTGCAACTTGAAGGGCGCTGCCATTAAGGAGGCCAAGGCAAAACTAGCCTTCGAGTTAACTGACGCTATTCATGGTACCAAGGCAGCTTTAGCCGCCCAAGCGAAAAGTGCCCGAATCTTTGCCTTTCAACCTGATACTGAAACCAAAACCGTGCGAGCTGGCACCAGGTTAGTGGATGTGATTGTTGATTTAGGTTTAGTTGTATCCAGATCAGAAGCGCGCCGGGTGATTCAGCAAGGTGGTTTAACGATTAACCAAGAAAAAGTGACTGATGTGGAAATGGTCCTACAAGCGAGTTCCCAACCACTAGTGATTGGTAAGGGCAAAAAGCGTTTTGTAACGGTGCAAGTGATAGCTAACACCAAATAAAAAACCATCTTTGAGAGTGGTCTTTTAATGAACTTAGTGTTATTTCTTAGCCTTCTTCGCTTTAACTGGTTTGCGCTTGGAATTGGTTGTGGGTTCAGTAACTTCAGCCACAACTGTTGTTTCTGTTGCACTGGTAGTTTCAGCGTTGTTGAAATCACCAATGAGTTTGGTCTTTAACAGTTCCACTACCTTATGGAATGAATCTAAGGTAGCAGCGTCATAACCAGCTTGACGGGTAATATTGAGTTCTAAATCACCCACAGCTTCCTCCATTCTTAAAGAAGTATCCATGAAGGTAATCGGTACACTCATCATGAGGTAAGTGTGTGTGGTATTGTTGTAAACGTTAAAGTTAGCATACACATTAACCGGGTTAAACAACACCTGCTTTATCATCTCCTTATCTAACTGATTAAAGTTAAAACTAGCGTCTTTTTCAGTGTAGAGCAAAACGTTGTTCTGGCGGTTACTTTCAGTTAAAGATTCGTAAAAACCAGTGTTAATTAAGGGGAAATCGCTTTTAGTTAAGAACCAGTGTTGGTCACTAACAGCATCAATAATAGCGACTAAGCAAGCCACCTTTTCATTTTTGGAAGTACTTCTCTTAATGGCACTTAACTTAGCAAAGTTAAGCGTTTGACCATTCTTGTACTGTACCTTAAATTGCTTGGGAACTAACTCAAAGTTATAGATGGTAGCAGGGTAAAAAGTCGGTAGAATATCCGCACTAATTGGACCTTTTTCAAACAATTCAAATTGCTCAAACTGCTTGTTGTTTGCCATCTTTTGTAACCACAAATCAGTACGATCTTGACGGTTGACCAAATGAATTAACTGACTGCGTTTGTACAAAGCTACAAAGTTAATCACAAAGCTCAACAGCAAGGTAAACCCGCCTAAGGCAATTAGGAAAAGAGCTGCCACTTTAGGAACAATTGAACCCTGACCAACATAGCTGTAAATCCCTAAAGAAAGGGCAATTAAAGCAAAGACACCACAAAAACCAACAACTCTTGTAAATTTATTGAACTTTTCAGGTGTTAAAAAGAACTTTTGAAAACGAGTTAGCTCTGGCATGCGTATATAAAAGATTGTAAGAAAAGATAAGTTAAACACAACGCTGGAGTTGTTTGCCCTACAGAACGTTTCCCTAAAAACTGCGCCTTGCCAAACTGCGCCCGGGTTTGACAAAGCCGTTCATACAGTTCAACTAACACCCCATCGAGCTGCTTGTACTGCCTTTTGGTGCGGTTTACTAATTGCAAGAAGCTTTTACAAAAGTAATACAACAAATCCCACACCCGTTTGTGACCTAAAGTAATTGCTTTAGTTTGTATCAATTTTTCGGTGTGTTTAACAAGGGTTTTCACCACGGTAATCACATCCTTGTTTTCTTCTAACAACAGGTATTCTACAGCCTCCCACCAAGTTAGCACAGCATTGGTTTCACAACCCAACAAGTTGGAGTGTTGCTTTAAGACTGCAATAAAGTTTTGCGGGGTTTTACCAACATCGTTGAGGGTGGTAAAACACTGAAAGCTATAGGTGCCCAAATCGGGTTGCAAGTGGTTGAGCTGCTTTTGCAAGTGCGCGAGGTTAACTAGGAGCTGATCGACAAAACTAGCAAACTTAACTGTTTGTTTCACTGTCTTTTTCTGTATCCTTCTCAGTCTTCTTATTTAACTTAGCTTCCTTGGCTTCTGCCTTTTGTTTGGCTTGCTGTTCTTGCTTTTGCGCTAGGATCTCTGGAGGGAGTTTAGTGTGTTCGTGAATGTAATCGATGTCACTCTTTAAGATGGTTTCGGCAATCAACAAGGCTTCAACTAACAATTCCAACTCCTTACGGTTCGTTTTAATAATGGTACGTGCCTTCTTGTACTGTTCTTCAATAATGGCGTTAATCTCAAAGTCAATATCCTTTGCTGTTTGCTCAGAGAACAGTTTGGTACCTGGTGGGACGGTACCTTGACTAGGTACATATTGCACTTGCCCTAACTTGGACATTCCTAACTGGGTAACCATGGCACGGGCAATATTGGTCGCCTTATAAAAGTCCGAGGAAGCGCCAGTGGTAATTTCTAGGGGACCGTAAATTTCTTCCTCTGCAGCACGGCCGCCCATGGCTGTAGCAATCATCGCTAACAAGTCAGATTTACGCTTTAAATTGAGATCGCCACTCTTAGGGGTTGACAAGGTATAACCACCCGCTTGACCCCGGGGAATAATGGTAATCTTTTGCACTTCATCGTTACTGTGAACATGGAGGCCAACCAAAGCATGACCTGCTTCGTGATAGGCCACTAGTTTACGATCAGCATCACTGACCACCCGAGATTTTTTGGCAGGACCAGCTATGACCCGGTCAATGGCTTCATCAATGTCGTTCATGTTGATCGTGGTCCGGTTATCACGGACTGCTAACAGGGTAGCTTCATTAATGACGTTTTCCAATTGAGCCCCACTAAAACCAGGGGTACGTTTAGCAACATCCAATAAGCTAATCTTGGAAGACAAGTTCTTGTTCTTGGCGTGCACTTGTAAGATGCCTTCGCGTTCCTTAATGTCGGGAAGGTTAATCTGGATGTGACGATCAAACCGACCTGGGCGTAATAACGCATCATCAAGGACATCCAAGCGGTTGGTAGCAGCCATCACCACAACACCAGTGCGGCTAGTAAACCCATCCATTTCCGCTAACAGTTGGTTTAAGGTTTGTTCGACCACCGAGTAGGAGGACAACTCCACGCGACCCCGTTTAGAACCAACCGAGTCAATTTCATCGATGAAGATAATACATGGGGCCGCTTTCTTAGCCTTATTGAACAAGTCCCGTACCCGTTTGGCGCCCACACCAACCAGCATATCTTCAAAGCCAGAACCCGTGGACTGGAAGAAAGGCACACCAGCTTCACCAGCAACAGCTTTCGCTAACAGCGTTTTACCCGTACCAGGAGGGCCGTAAAGAATAACACCGCGTGGGGAACGAGCGCCCATTTGGGCATACTTCAAGGGGTTTTTGAGGTAATCAACAATTTCCAGTAACTCGTGTTTTTCTTCTTGGAGTCCCGCAATGTTGTCAAACCTCACCGAAGACTTTGCGAGTTTAGCTTGGGTCTTACCAATTGAGAAGATGTTGTCCTCCTCACTGCGACCACCACCAGAGATACCGCGAGCAGAACGTCAAAAGAGTAAGAAGAAAATGACAAAGATAATAATTGGTAGTAGGCCAAAGAAGATGTTGAGCACATCACGTGCTCTCGTATCAGGAGCAACAAAGGTACCGATGTCATGGAGACCTTTAATAGAAGCTAATTCAGTGCCATTGTTACTTTTTTGGTCTTGGGTCATCATTCCATTAACTATGGCCTTTCCAGTTGACTGATTAATCGAGAGGTTTGCTATATTAAAGATAGTTTCCCCACTACCATTAGTAGCTTTGTGGGCAGTTACTACTATGGGATTTATCCCTTGGAAACTAATGGAAACCTGCAGAATGTCATCGGTGACATAGGTGGAATTATTAATTCTTTGGAAGGTGTGTTTGCCTGATAAGCCTTTGATTTGAGCCGAAAGTGTAGTTCCACTAAGCTCCCACTTTTCAATTACAGCCGTGGTAGCCCTTGGCATTAGGATATAAACCAAAATACCAATAATAATGGCTAGGATAACCACTCACCAAAAAATCTTTCACGCTGTGCGTTTGGGAAACTGCGGTTTTTCGCTCGTAGTCGCCTCGTTAAGTCCTTTATTTTTTTTCATTTGTTGTTGTTTGTTGGGTGATTTGACTGGTTTTTATTCTAACAACACTAAGGGTAACTAATACACCCACTGCTGCAAAACCAGCCAAAGTTAGAAAGGTAATTAAGATGATTTTTTTGTGCTTTTTTACCAGGGAAACCAACACACTAAACCATCGCATAAAATTCTAAATAGCGCGACTTAATTATCTTCAGGATCAAAGACACCAACATAGGGTAAATTGCGATAAAAGCCCTGGTAGTCTAACCCAAAACCAACCAAGAATTCGTCTTGGTTTTTAAAGCAAGAATAGTCCACCTTAATGTCAGCTTCCCTGGGTTTGAGCTTTTCAATGAAGCTCACTAAGATCACACTTTTGGCCTTTCGGGTGTGGAGTAAGTCCAATACTAATTTAATAGAACGACCACTGTCGATTATGTCTTCTATTAAAAGAATGTCCTTGCCTTTAGGGTCATGGGACATATCTAGGACAATCTTCGGTGCTTCTTGTTGGCGACTGCCACCATGGTAAGAAGCGACTGCCACAAAGTCCAGTTGTAAATCAAAGGTAAACTGACTAATAAGTTTGCCCAAAAAGGGAATACAACCCTTCAAGATCCCTAATACTATGACCTGTTTACCAGCAAAATGTTCATTACACCATTTGAGCGCAGCGTTACAACCCGCTTCAACCTGTTTTTGATCAATGATGATAGATTTAATGCCCATTACTTTAATCTTTGTCGTTCCACTAACACGACCGCTAAACAACTAATTTCATTCGAATTCAACGGTTCACGTACCTTGAGGTTAATTGTCAGTTCGGTAATCCGTAAAGCCGTACAAATAAAGTCAAAGATGGCATGCTTATAGTCATCCATCTTTTGTTGTGAGGGAATTTCTAAGGTGATATCAATGTTATTGATGTACCAGTTCTTGCGAATAAAGTTGTAAGTCCGTGCCAAAATACGGGGCGCTTCTTTACGCGGATTACCCTTTAAAGGGATCTGGGTTTTTTCTTTATTGAAGACAATTTGTCCATCACCTAAGGCAGTAGCACCAAATAAAGCATCAGCTACTGCTAGCTGGATAACGTCACTGGCGTCATCACTAGCATCGTGGTCATAAATGTACTTGGAATCTTCTATTTCCACCCCACCTATTCAAAATTTATTCTTCTGTTCTGCTCTCGGTTTAATGCGGTATTTTTTCTTACCTAGGCCAACTCTAAACTGCATCGTGGTATCTGTTAATTATACTTTTGCCCACCCCCTTTTCCTTTTATGTTGAGAGCTAAAAAAGACAAAGCTTGGCAGCAGGTTTTATTTAGATCAAATCTCAATTGGGAACAGTAAATGATTGGTGGCTTAAACCAAATTAAAATGAAGGCAAGCTTGAATAAATTAGTTATGAAGAGTCTTAAAGTAGCACTCATTGGTTCTGGTGCAGTGGGCACCAGCTTTCTCTACGCTGCCATGAGCCGTGGTTTAGCGAGTGAATACATGGTTATCGACATTAACGAAAAATCCCAAGTAGGGAATGTTTTTGACCTCCAAGATGCTGTACCATCATCACCGCAATACAGTAAGGTAATTGCTGGTGATTACAAACAACTCAAAGATTACGACTTTATCTTTATTGGTGCTGGTCGACCCCAAAAGCAAGGTGGGGAAACCCGGTTACAACTCCTTGAAGGTAATGTCGAAATTATGAAGAACATTGCCAAAGCCGTGAAGGAATCGGGCTTTAAGGGCATTACCTTAATTGCTTCCAACCCAGTCGACATTATGGCTTACACCTATTTAAAGGTAACGGGGTTTGAACCGAATAAGGTAATTGGTTCTGGAACCTTACTAGACAGCGCCCGGTTAAAGTTTGCGATTGCTGAAAAATACGGTATGAGCAGCAGGGATGTCCAAGCATATGTGTTAGGTGAACACGGGGATAGTTCAGTAAGTATTATTTCCTCCGCTAAGATTGCTGGCTTACCACTCAAACACTTCTCGAAAGCAAGTGATATAGAAAAAGAATTTGCTGAAATTGACCACTTTATTCGCCGTCGAGCTTATGAAATCATCGAACGCAAAGGAGCCACCTTTTATGGCATCGGGGAAGCAACTGCCGAAGTAGCAGAATTAATTCTCCGCGACACTAAAGAAGTACGAGTAGTAGCATCGTTAATTAATGGCCAATATGGTGCTAAAGATGTAATGTTTGGCACCCCTTGTGTCTTGGGACGCAATGGTGTGGAAAAGATCTTGGAAATTGAATTGTCTGCTACAGAAAAAGCAGGCTTGGACAAATCAATCCAAGTATTGAAGGACAACATTAAGTTAGCTAAACTATAAGAATTAGTTTAACTGTAGACATAAATAAACTAGTTAACTACTAGAACAAAACAAGCATGCTGACCCATTGGGGTTGATGTGCTTGTTTTATTTATTAAAAAATAGGCAAACTTTTTGGCGTATTTGTCAAATTCATTCAATTTATTAAGTGGGAGCAGGAAGACCGACTTTTGTTGAAAGTTTTTGCTGCTCCCGGCCAACTAGAAAAATATTTAAATTCATACATCTTAGTGTTTACAACTCAAACTAATATTCTTCGTTAGTTTTCAATATCGTTAAAAAATATTTTTTAGCCGTCTGCTTGTCAACTTGAGCAGATGGTTTTTTATTTATTTGAAAAGAATAAAAATACCAGCAACTCAACCTTAAAGATTAAATGCTGGTATTTTTAGTTTGAGTTGGAATTATTTAGATTCCAATTTGTCTATACGTTTGCCAAAGTCTATTAAGGTATTGTTAATTGATTCCAGGCTTTTAAGCACAAGACTTTCAAAGCGATCCATGCGGGCGTTCATTGCTTCACCCTGAATTCGAAAGCCTTCTCTAACTTCTATTTGGAATTCTTTAAATTCTGCCTTAGTAACATATCTTTGATCAAACTCAACTAAAAGCTCTTGTTTAAAAACTTCTAGTTTTTTGTCCAATTGTTTATGGGTTACGTATCCGCTTTTGCCTTTTTCCATATGGTTTAAATTGCCATAAAAGCCCATGTTATAGCTACCATCAGATAGATCTTCGATTCAGGCCTTTGCTGTTTTAATCTTATCTTTTTTCAAATTTCTTTTGAACAAAGTAATTGAAATTTCGCGTTTAATGGTCAAAACCTCCTTTCTTTACAGGATTTAAAAAAACAGAAAGCTTTATATGAGTGTTGGGCCAAATTTAAAGAGGGTCCAATTTTTCAAAAAATGTTAATCATTTCTTTTAAGCTCCTTTACTAAATAAATGGAAGCGGTTATTTAGAAGTACCAAGAAAATGAATTTTTAAATAGAAATCTGTAAAGAATGAAAATACCAACAGCTAACTGAATGTTAACTGTTGGCGTTTTCTGTTGAAGAAATGTTATTTCTTGTATTCAAGCCTATCTAAACGTTCGTTGATGGCTTGAAGGCTCTGAAGCACAACTTTAAAAGTTTCTCTAACTTCTACTTTGAAGTCTCTCAACTCCGCTTCAATGTTTGCGAACCTTCGATCAACCATGACCTTGTATTCCTCAAACTCTTTCCGGGTAACGTACTATGTTTTTGGCTTTTTCACTTTTCGTTGCCAACGTTCCTTAATTATAACATCAAAAGTTCCGTCTGGCAACGGAATCCAATTTGATTTTAATTCAAGAACCTCACACTGACTTCTAAACTTCTTGTATTCCCTTTCGGAATATCATTTGTTATTCAAAGTCAAAACCCCCTTTCTTACTTAAGTAATTAGAAAAAGAAGTAGACACTACCGAATTCCTTTTAGAAACAACAAATGTTGATGCGAGAACCAACGCAACAGATGCCTTTGCAAACGCGAAAGACTTATGCCCATAAGGGCTAATAAAAATGAGTTCATATGAGATTAAAGGGAGTTTTTACACTACCCAAGGTAATTAAATAGTGTTAAAGAACAATATAAAAGCCACCCCGAAGGGTGGCTTTTCCATATACACAGAAAGCGAACTAAATAAATAGGTTGTCCCATTCCGCTAACTGCAGTTTTATTATAAAAGAAATAACTAACCTTCGGAGTGGCTTTCTTTCTATACTGAGCAGGAGGCGACTAAAATAGTTGAATAATTCTGTCTACTGCAATCTAGTTATAAAAAACTCACCTAAAAGGTAGTTATTAACACCAAAAAAGCTTTTTAGAAGCGTTCTTAGTTTCAATTAAATAAACTCAGTGAATTAATTAAGCTTAACCAACTAACTTAGGCTTTTTCTTGAAGATAACGTTTAAGTATCAACTTCATGGTTTTGAATATCTTCTATCAATGCTTAAAGGCGGTAGAAAACGTTAGCCCACTTGTACAGTGCATTTTCAAAGAACAGTTCATTACTACGCTTTACCAACATACTGATTTCACTGAGGTTAGTGTGCTTGTTTTGCATAGTTTTAATTTTGAGCTCATTCTTATGACTACTTCAACCCTCATAAAAGCCCTTAAACTGATTGTTAAACTTCGTAATAAAGTACTTAGCATCCTTGTACTTGCTCGCCTGTTTTTCAAAGTCTAAGTCCCTCTGTTCCTTGGTTTCGTAAAACTTAATTTCGAACTTCAAACTGTTTAAATAACTGTCCAACAACTTCTTTAAGATCTTGTCCTTGGTGGTTTGGTACAAGTTAACAAAGAACGATTGGAACACATAGTCATCTAACTTAACAAACGCTGGCAGCATCTTTTTGTCAAAGTGTTCGTTCTTGAGTAGTGGTAAGCACACCTGTTCTAAGGTTTTATAACCGTTGAAGTCAAACTGGTTTTTGTCCACTAAGTCCTTAATTCGTTGAAAGACAAACCAAAGGTTTAACTCGGTGGAAATACTCCTGCCATCGCTGTAGATTGATTGGTACATGTGGTGCCGGGTAATTAAGAAGTTTTCAATTAAGGGCAAGCACTTATCCTTAAAGAAGATTTCGTAAATCCCCTTGACCTTGACACAGTCCATCTCCTTAATAATCGTTTGGTAATCTATCAAGGAATGACTGGTACCCGTAAAGTAGGAATCGCGCAACAGGTAATCCATCCGGTCAGCATCCAAGTCAGATGAAATTAACTGGCGCATCCAGTAGTTGGATGGCTTTAACTCCTTGTTTTCATCAATTAAAGCAGCTACTAGTTTAGGATCAATCTGGTTCGCCTTTAAGATTGATTTAATCGGTTCACTGTTGACAAATAAGGTTGTAACTTCTTCGTGGATAAACAGCTCCTTCTTAAAGTTAGGGGCTTTGGCAAAGTAAATCTCAAAGGCATGGGAATGCGGTGCATGACCAATGTCGTGCAGTAATCCCGCTACTAACACCGTTTGCTTTTTGTTTTCATTAATCGGAGCAAACGCGGGGGTGTTTAAGATCCGCCTTAACAGTTCATAAACCCCCAAGGAGTGGGAATAACGCGTGTGCACGCCCCCCGGATAAAAATGAAAGTTAATTCCTAGCTGCTTAATGTTACGTAAACGGCGAAACGCTTCCGTTTCCACCAACTCATACATCCACTTAGTTTGTTGGTCAAACAGCACTTCCCCTAAAATGGGATCTTTAAAAAAGATTTGTTGCATGAATAAATTCTTGAAGCAGCGCTAATACCTGAGTCTTACCGTGCAGCACCATGATGCCCGCCAAATCAGGACCATGCTCTTTGTTGGTAAAGATCAACCGTAGTGGCATAAACAGCTTTTTGCCCTTCAGATTAAACTGTTCACCAATTTGGGTTAAGGTACTTTTGACATTGTCCTTCGTCCAATCAGGTAGGGCTTGGAGTTGTTCACTCAGTTGTTGTAGGGCTGGTTGGAGCTCTTTGAAGAGTTCACGGTGCTTAACACTTAAATGCTGCACCCCTAACTTGGGGGCAAAGGTTTCCTTAATCAGTTGGTTAATTTCAATGCCAAACGCTAGTTGGTTTTTGAACAACAGACTGACTTCTTTGCGCTTGTCCTTGAGGTAATCAAAGTCAATCGTTAAGTAGTTATCAATAAAGTTAAAGTAGGCATTATCACTCAACTCCTTAATGTAGTGCGCATTAATTCACTGGAGTTTCTTAATGTCAAAGAAAGCGGGGGCGCCCACTACTCGATTGACCGTAAAGCTTTCAATTAGTTGGTTGATGGTAAAAAACTCGCGGTTAAAGTCCGAATTTCAACCCAGTAAGGCCAAGAAGTTTACCACCGCTTCTGGTAGGTAACCCTCTTGTTTGAACTGTTCAATGAACTGGATCGTTTTGGTATCACGCTTAGACAGTTTCTTGCCACTCTTGTCGACTATCACCGATAAATGACCAAAGCGGGGAATCCTTTGATAGCCTAAGGCTTGGTTAATTGCTAGTTGGTAAGCGGTGTTGGAAATGTGTTCCGCCCCCCGCAACACATCGGTAATTTCCATGTCATGGTCATCAATGACTACGGCAAAGTTATACGTGGCAATGCCATTGGCCTTCAATAAAACAATATCGGTGAGGGAGTTCCCGGGAATAGTAATCTTGCCCCGAACCTGGTCGTTTCAGGCAAACTCAGCGTCCTGGTTAATCTTTAAGCGAATCGTGAAGGGTACTTGGTTGTCTAAGTTTGTTTGAATGTGCTTGGAGTGTAAGTTGCGACACGTTCCCAAATACTTCGGGGTTTGGTGGTGTTCTAAAGCCAATTGGCGTTCGTGTTCTAACTTTTCTTTAGTACAGAAACAACGGTAGGCCAAACCCTTGCCCACAAGTTCATAAGCTAGTTTTTTGTAGGTAGCCAACTTTTGACTTTGGATGTAAGGCCCATAATTACCCGGGTTGTAAATTGATTCATCGGGAATAATTCCTAACCAGCGCAAGTTTTCTAGTTGTGATTCAATTCCCCCTTCCACATTGCGCTCAGTGTCAGTGTCCTCAATCCGGATAATAAACTCGCCGTTAAAGTGTTTTGCTAACAAGAAGTTAAAGATAGCAGTACGCGCACCCCCAACATGAAGGTAACCGGTAGGGGAAGGGGCATAACGGGTTCTAATCTTTTCCATCGCTTAAATGTGGTTTAAGGCTAAGATACAAACGAAACAACTGGGTCGGGGTTAACTCTTGGGCTCTAACCGTTGCCGCCAAGTCCTGTTGTTGTAAAACATTAGTTAACAAGGTGGGTGGGAGAAGCTGCTTGAGGTTATTGACTAACAGCTTGCGGCGCTGGTGAAAACACTGTTTTAAGAACCGGCCAAAATGAGCTTCATAGGCTACTTGGGGTTTCTTTTTTAACAACATTAACACCGAATCCACTTGGGGCTTGGGCTTAAAAGCAACCTTATCAATTTTAAAAACTGATTTAATTTGCAAATGGTATTGGCAAAACACACCAAAGGCACTATAGTTAGGCGAATTTACCGGTGCTACTAACCTTTGACCAAACTCCCACTGGCACACCAGCACAAATTGTTGTAGTTTGGAAGCCAAAAAGTTAGCAATCAAAGGGGAGCTAATACTGTAGGGAATGTTGCCACAAAGCAAAGGTATAGTGTCAGGAAAATACTGATCCAAGTTTTGTTTTAAGGCATCACCAATGGTTAGTTGTTCTTCAGTTAAGATTTCGTTGACCAAGAGGTATTCCGCTAAGCGTTTATCCAACTCTATGCCATGGTATGGTAGTTGTAACTTGAGTAAAGCCTTGGTTAACGCTCCCTTACCCGGCCCCACTTCAATTAAGGCCGTTGGGTTGAGACTCTTAATTAACCGACAGGTTTTGGCAATTACACTCTGGTCAACCGTAAAGTTTTGCCCTAATTTGCGTGAAGGATAAAAACTATTCACGTTCTAACATTTGTTCTAATTTGGAATACGTTAACCGTCTTCTGGTACGCCTTTTAACAATAAAGAGGTGGGTTAGATAAGACTGGAGTAAGGTAAACAAGGCATTTAAGAACCAGTACACACCCACACCAGCGGCACTAAAAGCCGTAATAACAGCAAACACAAAGTAGAAAATTAACTGCATTCGCTTTGTTTTATTGAGCTGTTCAATGCTCTTTTGCGAATGCGCTTTAGCATTCTCATTGCGTTTGCTAGCCCAAATTTGGGGCAACTTTTGCGAGATAAATTGCACTGGTAAAACAATGATTAAGAAGATAATAAAGGTTCACCCGGTACTGGTAAAGTTCGAGAAGATTTCCGTTAACGGTACCTTAGATAAGTCCCAGAAGTTAAACAGGATAATCGCTTTAATTGGTCTTAAAGTCGTGACAATCCGGTAAATAATGAGGAAGATGGGTAAGGTAACAAAGACCTGAACAAAGGAAGCAGAAGACTTAATGTTGTGCTTCTTGTACAAGGACATGATCTCCATCTGGCGGTTGCGTTTGCTCTGTAAGTCCAACGCCCCTTTGTACTTGGCATTAATTTCTGCTAATTTGCCCTGGACCTCGTTCATTTTTTCTAACGCCAGCGTGGAGTTGAGGGTAATCACTATGGTAATTAACCGTACTAGGAACAACAACACAATCAAAGACAAGATCATGTTGAAGCCCAATTCCGGACCGCTACCTAGAGGAACCCTGGTAGCGTACATAATCGGCAACACTATTTGCGATGCTGGTCAGACAAACCAACCATAAAAGGGACCATACGCTAAGGTATAGTCACTAAAGGTAAAGTAGGGCCCCACATTATTGGACTGCAAGTCATACCGGTAGTCCCCCGTAGTACCAAAGTTATAACCAATTTCTAACCCCGAACCTAACAGTTGGTTGGTTCCCGTTCAGGGCTGTGCTAGGGTTTGGGTACAACCCCATAAACCTACTATGGTTAAAAAGATAAAGATCGCTACTTTAAGGACTTTAAAGATAATGCCCCAGGCCTTTTTGAGATTTTTGAGGCCGTTCTTTTCGTTAACAACGGCCGCTGATCAAAAAGGGTTAAAGGTGGTTTTGAGCTCTTTATGCTTCTTATTTAGGTTTAGGGGCATCGAGTTCTTGGAGCTTTTGGCAAAGGTTTAAAAAGATCGTTTGCTTTTCTTTAAATGTTAATTCAAGGAAGCCTTGATTAATAATAACCAAAACATCAACAGCGTTTAAATTGCAAAACTGTTCCCGCATGATCGCTTTAACTTGCCGTCGAATTAAGTTGCGCTCAACCGCTAACTTGTATTTGGTCTTTGATATCGAAACCGCTGCCCTTCAAGTGCGGACGCGGTTCGGCATAAAGTAGGCCATCAAAAAGGTCGAGAAGAAGCGTCTTTTACTCCTTAGAAGGGCAGCAAATACTTTACGATCACGGAGGTGGTGCTTGGTCTTAGCGCTCACTGGATACGGTGAGTTGGGCGCGTTGTTTTTTTCTTCTTAACTTCAATACTTTACGACCAGAAGCAGTGGCCATGCGAGCTAAAAAGCCATGGGTTTTTGCTCGCTTTAATTTACTTGGTTGATATGTACGTTTCATGATACAGCGCTAAGTATTATAACTAAGATAAAGGACGCACCAATTCGTTAGACACTATCTTACCATCGGAGATCTTAATGATCCTGTTAGCGATCCGCGTTAACGTTTCATCGTGGGTCACCATTAGAATAGTTTGCTTATATTCCTTATTGATTGCTTGTAATAAGGCAATAATGTTTTCGGCTGAATTACTGTCTAAAGCCCCCGTAGGTTCGTCACAGAGCAACAAGCGCGGTTCTTTAATAATGGCACGGGCAATCGCCACCCGTTGCTTTTGACCCCCCGACATCTCGTACGGAAACTTGTACAAGATTTCCTTAATGTCGAGTTTAGCCGCTAACTCCTCGATGTCTAAGTGGCGTTGTTCTTTAGGAATTAGGTTTTGCGAGATGGCAATGTTGTCATACGCACTTAAGAGTTCTATGAGGTTATAGCGTTGGTACACATAACCCAGGTTTAACTTGCGGTAAGTTAAGAGCTGTTCGGTGGTACATTTTTCCAAGGGACAATTGGCCACCACACAGCTGCCCAAAGAAATTGAGTCATAACCGCCTATTAGGTTTAAAAGGGTAGTTTTCCCCGAACCTGATGGACCTAAAATAATCACAATTTCCCCATAACCGATTTCTAGAGAAACATCGGATAAGATAGCAATCTGTTCCTTGGAGTCCTCCCCGTGAACGTGCTTTCACATCTTGTCAATCGAGATCACCATCCCTTGTTTTTGCTCGACATAAGGAGTGCGGTAGGGGGCTTCCAGTTTTTCCTTTCACTGGGAGATCAACCCACCCTGCTTTTTCGGTTTTTTGGGTTTAGGTGCCTTGGTGGTCTTCGTGGGTTTAACTTGTTCCGGTTTAACTGCTTTCGCCTTCGGTTCCTTAACTTTTTTCACCTTAGCGGGTTTGGCCTTCTTTGTTTTCTTGTCTGATGCTAAATAATACTCAAAGGTGGCCTTAAAGCTGTTGGCCTTAACGTTTTCTAATATATCAACACACTCGTCCCAGACAAGTTCTTTCTCGGTTTTCTTTTCTGTATCTTTCATCCTAGAAGCCCTCCTTTAGTTCCGCTGAACGCAGTTTAATTCAGAAGTAAGAACCTATTACAAAGATGGTAGCACCAAACAGGACTATCCCCACCATGTAAGGTAAGAATTCAAACACGTTGGTTAAAAAGACCTGGGCAACCCCAAAGACTAGGGCTTGTACTCCTACTAGTAACCCAGCTAAGATAGCAATCGAGATTAATAACGAAAGTACAAAAGCGGGAATAAAGAAGCACAAGAGACTAACTAAGTTTTGACTATCTTTGTAACCAATCGATTTTAAGGAAATAAAGATCCGTTTCATTTCCTCTAACATCGAGATCCCCAAGACCACACAGGTAATAAAGATAAAGGGAATAATGGTGCCCAACAAGAACCCATCAACTTGGTTCACGGTATTAAACAACACCTCAGCATTATTCCTGATAATTTGAGCACTAGCACCAGCGGCATCTAATTTGAGAGCTGGCACCATGATGTTATCGCCAAAGATACCTCTAGCACCACTAGCTAATGATTGAGTTGAAGATCATTCGCTCGTATATTTTTCGATAACCTTTTTTCTGAGGTTTTCATAGGTCATGCTATCCACACCATTCTCACCCTTTTCCTTAGCCAACTGCTTTAAGATATCCGGATCACTTGGTAAGATTGAGGTAATAATCTTGTTTTCCTTACCCTTAATGAGGTTAGTAAAGTTATTACCAAAATCCGTAAAGTTACCCCAGACACCAATGTTGGAGTTAAGTACTAGCGCTTTACTTACCAAAGAAGGGTTGAGTTCCTTGGAGAAGACCCCGTTAAAGGGAACATAACCAGTTGGCTTGACGTACTCAATCTTGTACTTGTCTTTGGTGGTAGAAGAGGTACTTCCACCCCCACAAGAGCCACCGCCAGACGAAGTACCACCGCTATCATCGGTGGATTGGCCGTCCTTCACCACATCCTTAATGACCCCGCGGCCATCAAAGCCATTGAGCTTTAACAAGCGGTTGGCAATGTTTTGGTTAATATACAACTCTTGGCCAAAGGCATCGGTAGATACCCCGACAACCCGGAACTTCACGGTTGGTGTTTTAGGTGGGCTCTTTAAGAACTTCTCTGAATAGCGGGTCGCAGTGTTCTTGGGTTGAATAAAGATGAAGTCCCCGATCTTGGTGTTATTAGCGTACTGAAAGCCAGCGTTGACAATGACATTCATCTCACTGTCAGAGCTAATTAAGCTCCTCAAGGAGTTACCACCGCTGTCAGTGAGGTTGACAAACTTGGAATCGGGACTAATTCCCGTTAAGGTGTAAGGGGAATCAGGATTCAACTCGGTTTCCTTAAAACCAAATGGTCCTTGAACATAGGTGTATGTCTCCTCTATTTGTGGATCAACCGGGACAATCCCGTACGTTAACTTCGAGTCTTGGTAGGACAACTCCGGATCACCATAGACCTTAGTAAGGAATTTAAGGAATTGTTCGTTAAATTTAACTCCACCAGGATTGAAGACGTTAATCTGGGTTAATACCCTAGCGGCATCCAACTGAATAAAGTTAGTCCGAGGGTTGCGGGTTAAGAAACCATTATTGTTGAGGTTATTAAAGTAGTCATCAGTAAGAACCGCAATTAAAAACTGGTCACTGGAAGCCGATAACAATTGGGTAATTTGTTTAGGAATGGACTTCTCGACAATTTCCCACGGGTTAATGTTGGCACCACCAACGCGAATTGGGAAGTCAATGAGCCACTTCGACAACGAAGCATGACGGAAGAAGTTTTCGTCAGTGTTTAATTGTTGGGCACCACCAAAACTTGGGAGAAGGAGGTTACCGAGTTCGATTTCCTTACCATTATGCTTGATTGGGTTTCCGCCCCTATCACTAATCTTTAACTCTTTGAGGTTGTATGGGTGTTCTTTGTCGTTGCCTTGCTGTTGTTGCTGCTGTTGCTGTTGCTGAATTTGATCACCCTTATCTTTATACAAGGCCTTAATCCCCAAACTATCATCAGTTTGACCAAACCGGGAGTAGGGTTGGATTGCATACCAGCCGGATTGTTCTGTGGGGGTTTGCAAGTTCAACCTATAGTTAAAGTTGCGGTTTAAAACGGTGTTGGACTGCGCTGCCCCAAACTTTTGCGGGATGGTACCGGCAATCCCAATTAAAAGCAGGGCAATCGAACTTAACCCCACATAGGTAAATAACCGCGATAACCGGGAAATAATCAAGGACACCCGGAATTTACCCAAGGGAGTCATGGTGTAACTCTTGCGCTTTAATAAGTGCAAGAAGACCGAGAACTTGGTTTCATTACCCTGGTCAATCAGGACATTAACCGGTTTACTAAAGAGCTGTTTAAAGGCTATTCAGCTAATAAACTGGAAGATCACAAAGATCAACAGCATTGATCCAAAGAAGGAAATCCAGTTAAAGCTGTTGGCCGGTAGGGCTATAAACCAATACCTGGAGATAATCCCCTTAACTTGTCTTTCTAAAAGGAAGGCAAATAAGACCCCAAAGAAGGATGAGGCCAAGGCTACTATGCCGGCAAACACACTCATGCCGGCAATAAACTTGGCTGTGGAGAACCCACCAGCGCGGATAATTGAGAACTCCGTTTGGTTCTTCTTAATAAAAGACTTAATTAAGAGAATGGTGAGGTATAAGGCCAAGATGGATAAGAAGGCCGCAATGACCACACTGGCAATGGCTAAATAGTTCTGCACTAAGTCCGGGAAATAGTTCCGGGCTGTAGTTAAGTACTGGAACTTGGAGATGTCCTTAACGTTAAAGGCGAGATCACTGTCAGGGACGTTGAGATAGCCCTTTAACTTAGAGGCCACCACCTGGATCGGATCTTGGCCAGGGAACTTCGCTTTAATGTCATCCGACTTAAAGGCGTAGTAGTTCTCCTCACTAGCAGCAGGCACAGCAAACAAGACACTGCGGTATCCCTGCTCATTGAGAAAGATTAAGCCTTCATCCTGGGAGTTGGGCAGCGGTGAGTTTGTGGACAATACCGGATAACCGGTTTCTACCGACTCCCCAACCCCTAGAATGGCAAAGGTTAACCCGTTAATTGAGAACTTGTATTTGTCCGGTAAGTCATTTAACCAGTTGGGTAAATTCTTCGGGTTTTCTAACGCTTCTTTTCAGGAGTATTCCCCTTCTAAAGGTAATACTTCCTTGTCGTGTTTAAATAACCATTGCTGGGATACCACCGCACCGTGGGCTTCCGGGGTTTGGACTACCAAGGACTGTTGGTCGGTGGGTAGTGTTAACGGGTTAACCCCGTTTTTAAAGATAATCGGGATATCTAACTTTTGGCGGTTAGTTACTACCAGTTCATTCGGATCTTGAAACTCCGGGGTAAAGTAACTCAATAAAGCAACATAGTTGTTAGCGGCTTTACTCTTACCTGTATCATCTAAAGCCTCAAAGCCTTTTTTATCTAATGTAGACCACTGCTTAATGGCATTGGTTAAGGTATCAGGAATACTGGAAGCCTCGGGGAGTTGGGCTTGGGCTAAGATGTTAGCTAGTTTGGGGTAGATTTGGAATTTGGTAGAAGTACTCTTAGTATCACCAGTTGAAGAGGAGCTGGAGGAAGAAGATGCATCTGCTAACTGGGTTAAAGAGTCCGTTGTGCCTTGCGTAGTTTTGGCTGCTGCTTTTTTCTTTTCATCTGTTTGAGTAGGCTTAAACTCATACTCAACCTTTTCCAAGAAGCCTTCACTGTTACTCTTGCCCCCATATACCATGTCTAAAGGATTGCTTTTTTCCTTGGCAAGCCAAGACTTAAAGTCAATCCCTTCAATAATAGAAAGGTCGTTGTACTTCTGGGAATCCTTATTGGAGACCAAGAAGGAAGCGGATTCTTCGGAGCTAACATTGGTAGCAATCGCTAAGTTTTGCCGGGAAAACTCCAATTCCTTTAAGGCTTTCGCCTTTAAGAAGGCATCGCGGTATTTTTCTAATAACGCGGTGACCTTCTTCACCAGGTCTTCCTGGAAGATGCTTTTTCATTGGTGTAAAAGGGCAGCATAAACGTTATTTAAATTCTTTTGCGGAGTGTGGTTTTCGGTGCCAATTTTGTCGAATTCAGTTTGGACATCACTAAAAGTTGGATCTTTTACACCGTCATCTGCACTACTTTCCTGCTTATCTTGTTGTTGCTGAGCCAACGCAACGTTCGTTTGTTGTAATTGCGGCTTGAAAGAAACAGAAGAGCGAAGTGCAATCAAGCTAGAGGGATTGCTCTGAGTGCCAGACTTAGCATCGCCTGATTGTGTACTAAAAATCGTATTCTTATCACTATTACTAAACCCCGGTAAAGAATCTAAGATCGTTTCCTTTTCGGCTTTAACCTTATTTTTGAGCCAGTCCTTATTGATGTTAAGCTCAAACGATTTGAGGCTATTAATCTTCTTCTTGAGGTTATAGTCACCAAAGGAAGAACCGCTAGAAATAGTTTCTGCTTGCTTTTCTAATCAGTCAAATTCTTCCTTATCTAAATGAGATAACTTACTTGTATCTAAAGTAAAAGTTAAGGAGAAAGATTGTTTTTCTTTTTCCTCTTTTTCTTTTTGGAAGACCTCTATTATTTCTTTAAAGGGATTAGTCTCTAACCAAAACTGGGAACCCGGACTAATCGGGAGCACAATAGGTACATTAGCGTACAGATTGGGAAACCAGTTAGCAGCTTCTTGCCCTCCGGTAAATAGATAAGAGGTACTTAGTTTACTGGTTTCGCTTTCTCCACTGCTACTACCAGCTTGAGTTTCATCACTTTTAAACCAAACTTTGTCTTTATTATTGGTAGTGTATTGTTCTTCTACATTACTATCTTTTTTGGGCTCCTCGTTATTTGTGGTTTCTTTAGCATCAGTCATCTTGGCCTGAGCTGCTAACGGTTGTAAAACTGTTGTGGCACTACTCGCTTGCTTGATTTCTGATTCTTTTTTAGTAGTTTTCTTATCCTCACCCTTCCCTTTATCACCAGGAAAGCGAGCAATCCCAGCGCGCAGGGGAATCCCATCCAGAATTAAACCATCGACAAAGTAACCTAAAAGGTTACCTTCTTTGTCATAAATCAGGTTTTGGGGATCGGAAACCGTATTTTGGTTTAAGTTATTACTTTCTTGATCGGTTCCAGTAAAAGAATAGGTATTTTGAAAGGAAACAGCTAAACCCTTATAGATCCGTTTTTCGGCAATGTTGGCTTCCCCGTAAGCTTTCAAAATGCCTCTTTTACGAGCTAAAAAGAGTTTGTCTTTATCGTTAACATCACCTTTTCGTGGGTTATTATCTGATGATTTGGTGGTTGAATCTTTCTTTTCAGAATCACTCCCAAAATCAGATTCTTGGTAGGGCCAGATAAAGTTAATCTTCTTTTCGGTTAACTTGATTTTGGAAAAATGTCTTTTTTCTTCTTTAAGTTCCGTTTTATACACCGGACTACTTTCTTTAAAGATAGCCGGTGCTACTATGTCAGAAGAATTCGTTTTTTGAACAAGGGCAGTATAAGTATTAACAAGGTTATTACTTAACTGAAGAATCGACGTTTCCAGGAAGATAATGCTGAAAAGGACGAAAATAATCCCAAAAAGTAAAAAGAAGAACTTTTTGAGTGACTTAAAGATCTGTTTAAAAAACGAAAACATTTAGTTTAAACTCAGTTAATTTCGTTAATAGTCTTGGGTCTTTGGTTAAGATAATCAGCCACAATCTTGCCATCGTGGATCTTAATGACCCGTTTAGCTAGCTCCACAATCTTTTCATTGTGGGTAACTAATACTATAGTAGTGCCCTTATCGCGGTTATACTCCACAAAGAACTGGAGGATCTTTTTGGAAATATCCACGTTAACAGCACCAGTTGGCTCATCACCAAATAAAATACGCGGCTCTTTGATTAGTGCCCGCGCAATTGCTACCCGTTGTTGTTGTCCCCCAGAGAGCTTGGTAATCTTTTTATTACGGTGCTCTTTGAGCTCCAACCGCTCTAACAGCTCTTCCAAATTGTTGTTATGGCGCTTTTTAAAGGGAACTGCCAGTTTAATGTTGTCATCAACGTTCAGATCCCTTAAAAGGCCATACTGTTGGAATATATAACCTACGTTTTTATTCCGTAGTGATGTTAATTGGGCATCATTACAACAAATAGTACTCCTACCACACACAAAACTCACCCCGCTAGTAGGTCGGTCTAGGGCGGAGATTAAAGAGAGTAAACTAGTCTTACCAGAACCAGATTTACCCAAAATAACCACAAACTCCCCAGGGGCAATTTTGAGATCAATGTGATCACAGATTAACTGGTTTGTGATCCCATTGGTCACTGCCTTACAAACGCCCTTGAACAAAACATCGTAATGTTTAGCCTCAGGGTCTTTGTAGTGCCGTCTTTTAGGGTGCTTCTTATTTAACTTAACGATTAGCTTTGACGCTTTTTTACTTTGACAAAAATCGGGTCATTGCAAACAATAATCGAGGCTTCGTTTATTTTTAGCATTTAAAGACATTGAAACATGTCAGGTTTAACAACGGGACAGGCCAGTTTCAATTAAACACTTAAAGAATTATAAAAGGACTAAATTACCAAGGAAGTGATAATGTCTTGGAGGGAGTCGTCCTTCTCAATCATTTTAGCAACCCTTTTAACTGCAGCCACCACTGTAGAGTGGTTCCTTTTAAAAATTTTGCCTATTTGTTGGAACTGCATCTTGTACTTTTCCCTTAAAAGATAGTTACAAATGTCACGAACACGAACTAACTCTGCTTTACGGTTCTCAGACAAAACACTGTCCACCGGTACATTAAAGCGGCGGCAAACGTTTTCTATTAATAAATAAGGATCAAAGCTCTTTTTGTGGAACTTCTCAAATTCCTCAAACAGGATTTCCTTTAGATTGTCGTTGTTAATCAGGTTTTGCTTAGTTGTTTTGACATAAAACAGCAACTTAGTCGCAATCCCGTTTAAAGAACGAACATCGTTACCAGATATTTGCGCTGCCTTTTCGCGTGCTTCATTGGTAATTTGGATGTTGGGATCCTTTTCTTTGAGCTTAACGGCCAAAATTTCACACAGGGAGCTCAAATTGTGTTTTTCAATCTTTAACAAGAGTCCCGATTTAAAGCGTGATACCATCCGTGGATCGATGTTGGCCAACTCATCGGGGGTTTTATCGGACACAAACACAATTTGCTTGCCCTTACTTACTAAGTTATTAAAGATGGAAAAGAGGATCTCTAAGGTTTTTTCCTTTTTCCCGAAGATCTGAGTATCGTCAATTAAGAGTAAATCCAATGACTCGTAAGCAGCCTTGAGCTTTTCAATGCCTTGATCCTTGTCCTTTTGGTAAAAGGCATTGACAACCTCCTGGGCAAAGTCACTCGATACCACATACTTTACCTTAGCGTTGGGAAAGTGGAAGAACTTATCATTCCCAATCGCTTGGAGTAAGTGGGTTTTACCCAGACCAGTCTCCCCATAGATAAATAAGGGGGAGAAATCAGCGTCCTGAGTTTCGCTAATCCGTACACCCGCTTCATAAACCCGGCGGTTGCCATCACCAACCACAAAGTTTTTAAAGGTAAAGTTTTTGCTTAGCCCCGAACTTTGGTAAAGCGTTTCGCGGTTATCTTCCTCTAGTTTGGCTAAATTGATAAAGAAGTCCTGTTCGTTGACAAACCGAACTGCTCTAATGTCATCGTACAGTTTGCTAGCTAACCCGACAATATCAGGGTTGCTATTGAGGGTTTCACGGGCAAACTCATTGTCCACAATCACAAAAAGGACATTATTTTTTAAAGCAAACCGTTTAAGATTCTTAATGTACTTGTCATAAAAACCTAAAGTAGTTTCGTATTGCTTTTTTAAAAGAAGTTTAAAAGCACTAAATTGTTCCATTACTCTTCTATTACCTGATCAAGAATCATCGTTAAGATCCGCGAAATCGGAATGTTAACCGCTTTAGCATAACGCTTAACCTTATTCCAAACACGGGTACTCGTATAAAAGTGCACCCCGGTGGTTAAGCGTTCTTGCCGTTCTAAGGGAATAATACCGGGCACTTTCGCCTTTGGTGGTGGTAGATTTTCTTCTTCCTGCAATTTATTAGATGGGCGAGTAGTCTGCTTAACTTGTGGCTGTTCAACCGCTGGTTGTGGTTCTGGTTGGGGAACAGGTTCCACAATTGGTTGCGTAGCCTCCACCTGAATATTAGATGTAGCTACTGGTTGTTGATTAAAGTTAGTGGTTGTAGGTTGTTGAAACTCATTTAAGTGCACTTCTGCAGTTTGGTGAACTGGTTGGGTTGGAACCTGATTATTAAAGTTAGTTGGTTCTGATTGCGGTTGCACATTAACTGGTTGGGTTGAAGTTTGTGGTTCTTGTTTGGGAAACTGGTTAAAGGATTTCACAAACTCATCGCCAAACTTGAGTAATGGTCCAAAATCAAACTGGGGCATGTCCTTAGAAAAGAACTTTCTGTCTTGACTAGATTGATTTGCTTTAGTTTGGTTAGTTAGATCATGTTGGGGTTGGACAACCGGTTCTAGTTTTAATTGTTGTTCTACCTTTTTAACTTCTTGAACAGTTTCTGCATCATCTTCATCCTGAGCTAGGTCAAAGAAGAAGCGTTTCTTTTTACTGATGGCCATGGTTGACTAGGTTAAGAATTTCTTTAGTAATCTCTAAATATTCAGTTTGGTACTTCTTGTTGGATGTTGGTGATACCAAAGAGATAGGTAACTTTTCATAACCAACAGCAGCAGATGACTTAGATGTTAAAGATACAAAGTGTTCTGAAAAAGCAACGTTGTTCTTGTGCGCTTTTGATTTCACCAACTCAATCACATCATTGTGTAATCTGGTGCGCATATTGACCTTGGTTGGTACCAAAATGGTTTTTAAGTTGGGGTTCTTTTCCTTAAAAGTATCAATCGTTTCGACAATCCGCATTAAACCCAACATAGAGTATTGATCAGGTTCAAACGGGATCACAATCACATCAGAGAGGCTCATGGCTGTGGAGACCAAGGTAGCCATGTTAGGCGGTGTGTCTAGTAAAACAAAGTCATATCTGCGGGTGAGTTGTTTAACTATTTCCGCAATATCAGAAGCCTTGTACTTTTTGCGAGCAATATCAATGTCCGCAAAATTTAACTCAAAGTTACAAGGCAAAATGTCTAGTCCTTCATAAACGGGCAACAAACAGTCATCAATTTCAATTGAACTGTTGGCGCCGTTAAACTTAGGCACTTTTAACAAAATATCAATTAAGGTGTTATTTAATCTTTCTGGATTTTGTCCAAAAGAGGCTGAAACGTTGCCCTGACCATCAAGGTCTAAAATAACTTTCCGTTGTTCAGGGCACAACTTTACAAGCGAACCAGCAACGTTAGTGGCCATGGTTGTTTTTAAAACGCCACCTTTGTTATTAACAAAAGAAATAATCATATGCGCGCGTATAAGTTTGCTTAAATTCTAATATTTATTTAGTATTATTCCGGTAT", "length": 818445, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_009939745.1|CP039761.1", "orig_description": "HMT-732 Mycoplasmoides pneumoniae 16-734" } ], "run": { "start": "2026-01-27 02:05:34", "end": "2026-01-27 02:12:43", "duration": "7.15 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }