{ "genome": { "genus": "Metamycoplasma", "species": "hominis", "strain": "FBG", "taxon": "Metamycoplasma hominis FBG", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 780024, "gc": 0.26988272155728543, "n_ratio": 0.0, "n50": 780024, "n90": 780024, "coding_ratio": 0.7233162056552106 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 472, "stop": 621, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTCAAGCAAAAATGGAAGAATTCAACCAAAAGAAAGCCAAAATTGAATCTAAATATGCTGCTTACAAAGGCGACAGACAAATGATGCAACGTAAGCAAGCTGAAATTAGTGAATTATATAAGAAAGAAAAAATTTCTCCTTGA", "aa": "MFSQAKMEEFNQKKAKIESKYAAYKGDRQMMQRKQAEISELYKKEKISP", "aa_hexdigest": "7588ca6ca96a15cae5b57833b385386d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5875.7765, "isoelectric_point": 9.646807289123537 }, "id": "DFOKDJOJIG_43", "locus": "GCA013367855_00001" }, { "type": "cds", "sequence": "contig_1", "start": 850, "stop": 1002, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTCAACTATTTACTTTTGTTAAAAGTGTTTTCCAACAATACTTATCAAATGTAAATTATGTAAAATTACCTTCTAAAACAAATAACGGTAGTTTTGTATATAGAAAATCAAGTCTAAGTCCTGCTTCAAATTACAATTCAGAATATTGA", "aa": "MLQLFTFVKSVFQQYLSNVNYVKLPSKTNNGSFVYRKSSLSPASNYNSEY", "aa_hexdigest": "1b513fab48c3872b6217a895eff70ee6", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5790.470700000002, "isoelectric_point": 9.698317527770996 }, "id": "DFOKDJOJIG_44", "locus": "GCA013367855_00002" }, { "type": "cds", "sequence": "contig_1", "start": 1300, "stop": 1434, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGATGAAAAGGGAAATTACAAAACCAAAAATTACACTCCATTAACCAAATTCAGTGAAATTTTAATTCCTAATTCTTTATCGTTTGACACTAAGCCAAAAGATAAAGATGGCATTATACCAACAGATTGA", "aa": "MGDEKGNYKTKNYTPLTKFSEILIPNSLSFDTKPKDKDGIIPTD", "aa_hexdigest": "00a76826a52447d9c1fa25fe72451b42", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4961.553500000002, "isoelectric_point": 6.104928398132324 }, "id": "DFOKDJOJIG_45", "locus": "GCA013367855_00003" }, { "type": "cds", "sequence": "contig_1", "start": 1513, "stop": 1854, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTATCGGTTTAGTTGGTTGTGTACAATCATTTACTGTAAAATCAAGTAATAAAGTCGGTTCTGGACAAGAGCTATATTCTAAAAAGAGTGACATCTCACCAAATATTGTCACTTATAGATACGATAGCACTGGTAATTTCTTTACCTTACCAAACAAAATAAATGATAAAAATATTATCGCCAATACATATCTTGCTTTAAAAGAACCTAATGCTATTAATGAGTTAAGGAAGAGAGATCCTAATTATGGCGCATATGGTTCTCGTTCATTTGGATTGCAATTGCAAATGCCAAAAAACGACAAAGCAAAAGTTTTATCTTCTCATGATGAAGATTGA", "aa": "MAIGLVGCVQSFTVKSSNKVGSGQELYSKKSDISPNIVTYRYDSTGNFFTLPNKINDKNIIANTYLALKEPNAINELRKRDPNYGAYGSRSFGLQLQMPKNDKAKVLSSHDED", "aa_hexdigest": "200f4ab9d28dac35760f2bb7d475a211", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12536.983699999986, "isoelectric_point": 9.211774635314942 }, "id": "DFOKDJOJIG_46", "locus": "GCA013367855_00004" }, { "type": "cds", "sequence": "contig_1", "start": 1970, "stop": 2299, "strand": "-", "frame": 3, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "COG:COG0594", "COG:J", "EC:3.1.26.5", "GO:0000049", "GO:0004526", "GO:0008033", "RefSeq:WP_020002379.1", "SO:0001217", "UniParc:UPI000368424E", "UniRef:UniRef100_A0A454C8Q8", "UniRef:UniRef50_A0A448ZZD2", "UniRef:UniRef90_D1J7D9" ], "nt": "ATGATAAATAAAAATAACGTTGTTAAAAAAAATTGGGAATTTCAAAAAATTCTTGATTCTCACAATCAAGTGATTTCAAGAAATTTAATAATTTATTTTAACAAATCCGATTTCTTTAAAGTGGGTATTTCAATCCCTAAGCAATTTGCAAATGCTGTTTTGAGAAATCATTACAAAAATCAAATCAGATCAATTCTAAGAAACATTGATTTTAGCACTTTAAATTACGAAACTGTGATTATTGCTCGCAAAGGCTTCTTGAGTCTTGACTGGAATCAAAAAGTTAAAGAAGTAAAAAAACTTTATGAAAGGATCGCTGATGGCAAATAA", "aa": "MINKNNVVKKNWEFQKILDSHNQVISRNLIIYFNKSDFFKVGISIPKQFANAVLRNHYKNQIRSILRNIDFSTLNYETVIIARKGFLSLDWNQKVKEVKKLYERIADGK", "aa_hexdigest": "5aaf002c4988c04a976148e8c285e553", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000368424E", "ncbi_nrp_id": "WP_020002379.1", "uniref100_id": "UniRef100_A0A454C8Q8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000368424E", "RefSeq:WP_020002379.1", "UniRef:UniRef100_A0A454C8Q8" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C8Q8", "uniref90_id": "UniRef90_D1J7D9" }, "psc": { "uniref90_id": "UniRef90_D1J7D9", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_A0A448ZZD2", "cog_id": "COG0594", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0004526", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZD2" ], "product": "Ribonuclease P protein component" }, "genes": [ "rnpA" ], "id": "DFOKDJOJIG_47", "locus": "GCA013367855_00005" }, { "type": "cds", "sequence": "contig_1", "start": 2327, "stop": 2473, "strand": "-", "frame": 3, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "KEGG:K02914", "RefSeq:WP_012855284.1", "SO:0001217", "UniParc:UPI00006920BF", "UniRef:UniRef100_D1J7E0", "UniRef:UniRef50_P05647", "UniRef:UniRef90_B3PNJ7" ], "nt": "ATGAAAAGAACATACCAACCAAAAAAACGCAAACACATTAAAGTTCATGGTTTTATGGCTAGAATGCAAACTAAAAATGGTAGAAAAGTTTTAGCAGCTAGACGTGCAAAAGGTAGATATACATTAACTGTATCAGACAATAAATAA", "aa": "MKRTYQPKKRKHIKVHGFMARMQTKNGRKVLAARRAKGRYTLTVSDNK", "aa_hexdigest": "c12d043f3061d758a33db3c492b0b7ea", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00006920BF", "ncbi_nrp_id": "WP_012855284.1", "uniref100_id": "UniRef100_D1J7E0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00006920BF", "RefSeq:WP_012855284.1", "UniRef:UniRef100_D1J7E0" ] }, "ips": { "uniref100_id": "UniRef100_D1J7E0", "uniref90_id": "UniRef90_B3PNJ7" }, "psc": { "uniref90_id": "UniRef90_B3PNJ7", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_P05647", "cog_id": "COG0230", "cog_category": "J", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P05647", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05647" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "DFOKDJOJIG_48", "locus": "GCA013367855_00006" }, { "type": "oriC", "sequence": "contig_1", "start": 2474, "stop": 2805, "strand": "?", "product": "origin of replication", "nt": "AACAATTCTCCTTTGCGTTTTTATATATAAAATAACTTAATTTATTTTACCAAAAATACGACAAAAAAAGTATATAAAATACCTAAGAAAAAAAATTTAATATTTTTTATATTTTTAATGTAAATTAAAAGAAACATAAAATTTAAAGTTAATAACAAAATAAACAAAGAAATTATTTAAATGTTTATTATGTTATTTTAGTATAAAAAACCTCTTATTTTAAAAACAAAAGCCCTAAAAAAAATGTTAATAATTTTGTTAATAATTTGTAAATTAGCATTTATAAGGGCTTATTAATTTATTAATTTTTAATTTTAAAATATAATAGAGGT", "id": "DFOKDJOJIG_1377" }, { "type": "cds", "sequence": "contig_1", "start": 2806, "stop": 3735, "strand": "+", "frame": 1, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "KEGG:K02313", "SO:0001217", "UniRef:UniRef50_A0A448ZWT6", "UniRef:UniRef90_D1J7E1" ], "nt": "ATGAAAACTACAAATCCCTCTGAATTAGATTTAAGAACACATAATATGTCATTTCAAAAAGAATTAGTTAATACTGCAAGTGATCCTTTAATATATGATCAATTCTTGTCGTCATTAGAAATTGTAAAAGAAAATAATAAAATGATTTACATATTAGTTAAAGGACCTGAAGACGTTTCAACATATATTAAGAACAGTCACAATGAAAGTATTAAAAGAGCAATTAAAAATGTTTATGGTGAAGAATATAATTTAGTTTATATTAGAAATTTAGATGAAATTAAAAGCTTGCAAGATAGCAATAATGTTGAATCTGCAACTAATTCATTGGCTAATAATTCTAAAGAAAATTTTAGCAATATTAAGAAGAATTTAAGATTTGATGATTATGCAATAGGAAAATTTAATAATATGGCTTTAAAGGCAGCAAAGGCTATTTGCAATAGTGAAAAGATATTGTTCTCTCCTTTATTCATTCATGCTTCAAGTGGGCTTGGTAAAACACATCTATTACATGCTATTGGAAATGAATTATTAAAACACGGTAGAAGTGCATTATATATAAATCCTGATTCATTGACAAGAAGATTAGTTGAACAATTAAAATCAAAGAATCAAGAACAAATTAATAAAATTATTGATGAATTAATGTCTTATGATTGCCTAATGTTTGATGATGTTCAACAATATGGTAATCGTGATTCTACATTAAATGTCTTATTTAATATTATTGATAATATGATAATGAACGATAAGCAAATAATCTTTTGTGGTGATAAAAAACCAGATGATTTAGGTGGATTTGAACAAAGATTTATAACTAGATTTAATGGCGGATTAACTGTTGAAATTTTAAAACCAGAGTTAAATGATGTAATTAATATCTTGAAATTTAAATTGCAAGAAAACGGTATAAATCCGGAACTTTGA", "aa": "MKTTNPSELDLRTHNMSFQKELVNTASDPLIYDQFLSSLEIVKENNKMIYILVKGPEDVSTYIKNSHNESIKRAIKNVYGEEYNLVYIRNLDEIKSLQDSNNVESATNSLANNSKENFSNIKKNLRFDDYAIGKFNNMALKAAKAICNSEKILFSPLFIHASSGLGKTHLLHAIGNELLKHGRSALYINPDSLTRRLVEQLKSKNQEQINKIIDELMSYDCLMFDDVQQYGNRDSTLNVLFNIIDNMIMNDKQIIFCGDKKPDDLGGFEQRFITRFNGGLTVEILKPELNDVINILKFKLQENGINPEL", "aa_hexdigest": "80574c700fe36dbc574b01c57ab4963a", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaA" ], "truncated": "3-prime", "pseudogene": { "start": 2806, "stop": 4038, "observations": { "mutation": [ 3733 ], "stop": [ 3733 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E1", "query_cov": 1.0, "subject_cov": 0.6673866090712743, "identity": 0.9940000000000001, "score": 598.0, "evalue": 1.36e-213, "gene_start": 1, "gene_end": 309, "reference_start": 1, "reference_end": 309, "reference_sequence": "MKTTNPSELDLRTHNMSFQKELVNTASDPLIYDQFLSSLEIVKENNKMIYILVKGPEDVLTYIKNSHNESIKRAIKNVYGEEYNLVYIRNLDEIKSLQDSNNVESATNSLANNSKENFSNIKKNLTFDDYAIGKFNNMALKAAKAICNSEKILFSPLFIHASSGLGKTHLLHAIGNELLKHGRSALYINPDSLTRRLVEQLKSKNQEQINKIIDELMSYDCLMFDDVQQYGNRDSTLNVLFNIIDNMIMNDKQIIFCGDKKPDDLGGFEQRFITRFNGGLTVEILKPELNDVINILKFKLQENGINPELWEEESLKFIARNFSSSIRNIEGAINRIKLFQDGDDGFFTYDINTIKSIFNSMTQVAENITPDKIIDAVSKYYKIDRKKILSNVRTNDVVNARRIATYLVNKNFNYTLQEIGKVVGNQSHSNVIVSLNWIEKNANSNPTLKLALQKIESNLKKIS" }, "paralog": false, "identity": 0.9926829268292683, "score": 800.0, "evalue": 1.87e-295, "description": "Internal stop codon at 3733. Nonsense mutation around 3733.", "psc": { "uniref90_id": "UniRef90_D1J7E1", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_A0A448ZWT6", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E1", "UniRef:UniRef50_A0A448ZWT6", "COG:COG0593", "COG:L", "KEGG:K02313" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZWT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZWT6" ], "product": "Chromosomal replication initiator protein DnaA" } }, "id": "DFOKDJOJIG_49", "locus": "GCA013367855_00007" }, { "type": "cds", "sequence": "contig_1", "start": 3886, "stop": 4116, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08299.17" ], "nt": "ATGACTCAAGTTGCTGAGAATATTACCCCCGATAAAATAATTGATGCTGTTTCTAAATACTATAAAATTGACAGAAAAAAAATATTGAGTAATGTAAGAACAAATGACGTGGTTAATGCCCGCAGAATAGCAACATATCTAGTGAATAAAAATTTTAATTATACATTGCAGGAAATAGGCAAGGTTGTAGGAAATCAATCACATTCAAACGTTATTGTTTCGCTTAATTGA", "aa": "MTQVAENITPDKIIDAVSKYYKIDRKKILSNVRTNDVVNARRIATYLVNKNFNYTLQEIGKVVGNQSHSNVIVSLN", "aa_hexdigest": "28ae5fe87d266549f489ee290f7117cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08299.17", "name": "Bac_DnaA_C", "length": 66, "aa_cov": 0.868421052631579, "hmm_cov": 1.0, "evalue": 2.7551341572028484e-21, "score": 71.04753875732422, "start": 10, "stop": 75 } ], "seq_stats": { "molecular_weight": 8637.7939, "isoelectric_point": 9.753437995910645 }, "id": "DFOKDJOJIG_50", "locus": "GCA013367855_00008" }, { "type": "cds", "sequence": "contig_1", "start": 4213, "stop": 4611, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00712.24" ], "nt": "ATGAAATTAAAAATTGATAAATTGTTATTGGATAATGCAATTGAAAGAGTATCAAAATCTGTGGATGCTAATCCATTTTTACCAGTCATGAAAGGTATTTTAATTCAAGCAGAAGATAACAAAATAACATTAATTGGTTCAAATGGTGAAACAAGCATAAAGCATGAAATAGAAACATCGATGGATGCCCAAATTGAATTGCCCGGAATTGTATTAGTCGAACTTAGCTTGTTTAAAAATATTATTAAGAAGTTAGATGGCGAAATAGTATTAGAAGTTGTAAATAATATTCTTAAAATAAGTACTAACAACGAAAATTATAATTTAAATATATATGATGTAAATGAATATATTGCAATTGATTTTGCATGTTATGGGGAACAAGTAAAAATTAACTGA", "aa": "MKLKIDKLLLDNAIERVSKSVDANPFLPVMKGILIQAEDNKITLIGSNGETSIKHEIETSMDAQIELPGIVLVELSLFKNIIKKLDGEIVLEVVNNILKISTNNENYNLNIYDVNEYIAIDFACYGEQVKIN", "aa_hexdigest": "9ef52e86224a71ce7e6a51915943094c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00712.24", "name": "DNA_pol3_beta", "length": 122, "aa_cov": 0.9090909090909091, "hmm_cov": 0.9754098360655737, "evalue": 3.1435706623801592e-18, "score": 61.724979400634766, "start": 1, "stop": 120 } ], "seq_stats": { "molecular_weight": 14804.990099999985, "isoelectric_point": 4.6782129287719725 }, "id": "DFOKDJOJIG_51", "locus": "GCA013367855_00009" }, { "type": "cds", "sequence": "contig_1", "start": 4678, "stop": 5310, "strand": "+", "frame": 1, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360", "KEGG:K02338", "SO:0001217", "UniRef:UniRef50_A0A2Z4LLI0", "UniRef:UniRef90_D1J7E2" ], "nt": "TTGTGCTGTGTTAATATATCAGCAGCCAATAAAAAAATAAAGTTTATAACAACAGACAGATTTAGATATGCTCAAGCAGTTCAAGACATTTCTGAAGATGTAACATTCAATATTTCAATATTAGCTAAAAACTTAAGAGATATTACCAATTTTGATTACAATGGCGATGTTATTTTAAATATTTCTGATCAAAAGATTTCATTTAAATTAGACTCAACAACTATTGAATCAAAAGTTGTTGATCAATTGTATCAAGATGTTTCAAGAATTGTTCCACAAGAATTTGACACTGAACTTAAAATAACCAAAAAAGAACTAAATAACTTATTGAATAAGGCATCAGTTATTATTAATGAAAACTATAATAAAATTAAGTTGCACATTGTTGATAATGTTTTAACCATTTCATCTGCTAGAGAAGAAATTGCTAATGCTGAAATTAAAACAAATAATTTTTCTTACAACGGTGAAGAATTGAAACTTGCATTAAATAGTAGATTTTTAAAGGATGCTATTTCTGTATATGAAGATAATTTGAATATTTTATTCACAAAAGATAAATTAAGAATAGTCATAAAATCAGATTCTAACCCAAATGTGCTACAATTGATTACGCCACAAAAAGGATTCTAA", "aa": "MCCVNISAANKKIKFITTDRFRYAQAVQDISEDVTFNISILAKNLRDITNFDYNGDVILNISDQKISFKLDSTTIESKVVDQLYQDVSRIVPQEFDTELKITKKELNNLLNKASVIINENYNKIKLHIVDNVLTISSAREEIANAEIKTNNFSYNGEELKLALNSRFLKDAISVYEDNLNILFTKDKLRIVIKSDSNPNVLQLITPQKGF", "aa_hexdigest": "5fd6dec55127547b14c9da8b30a299ed", "start_type": "TTG", "rbs_motif": null, "genes": [ "dnaN" ], "truncated": "5-prime", "pseudogene": { "start": 4378, "stop": 5310, "observations": { "mutation": [ 4609 ], "stop": [ 4609 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E2", "query_cov": 1.0, "subject_cov": 0.5753424657534246, "identity": 0.995, "score": 399.0, "evalue": 5.1e-138, "gene_start": 1, "gene_end": 210, "reference_start": 156, "reference_end": 365, "reference_sequence": "MKLKIDKLLLDNAIERVSKSVDANPFLPVMKGILIQAEDNKITLIGSNGETSIKHEIETSIDAQIELPGIVLVELSLFKNIIKKLDGEIVLEVVNNILKISTNNENYNLNIYDVNEYIAIDFACYGEQVKINWNDFKSLVRDVIFAASTNEVSLILCCVNISAANKKIKFITTDRFRYAQAVQDISEDVTFNISILAKNLRDITNFDYNGDVILNISDQKISFKLDSTTIESKVVDQLYQDVSRIVPQEFDTELKITKKELNNLLNKASVIINENYNKIKLHIVDNVLTISSAREEIANAEIKTNNFSYNGEELKLALNSRFLKDAISVYEDNLNILFTKDKLRIVIKSDSNPNVLQLITPQKGF" }, "paralog": false, "identity": 0.9935483870967742, "score": 592.0, "evalue": 2.35e-216, "description": "Internal stop codon at 4609. Nonsense mutation around 4609.", "psc": { "uniref90_id": "UniRef90_D1J7E2", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A2Z4LLI0", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E2", "EC:2.7.7.7", "UniRef:UniRef50_A0A2Z4LLI0", "COG:COG0592", "COG:L", "KEGG:K02338", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z4LLI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z4LLI0" ], "product": "DNA polymerase III subunit beta" } }, "id": "DFOKDJOJIG_52", "locus": "GCA013367855_00010" }, { "type": "cds", "sequence": "contig_1", "start": 5310, "stop": 5483, "strand": "+", "frame": 3, "gene": "ybcJ", "product": "Ribosome-associated protein YbcJ, S4-like RNA-binding protein", "db_xrefs": [ "COG:COG2501", "COG:J", "SO:0001217", "UniRef:UniRef50_N9UZE0", "UniRef:UniRef90_D1J7E3" ], "nt": "ATGGAAATAGAAATTACTGGAGAATTTATTAAATTAAGTCAATTTTTAAAGAAAATTGATGTGTGTCCAACGGGCGGAATGGCAAAATATTTTGTTCAGGTTCATAGAATATTAATTAACGGGAACCTTGCAAACGGAAGAAATGCTAAGATTTATCCCGGCGATACCGTTTGA", "aa": "MEIEITGEFIKLSQFLKKIDVCPTGGMAKYFVQVHRILINGNLANGRNAKIYPGDTV", "aa_hexdigest": "c366bbf7c6e153c1c5ccd4e3f3610b33", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7E3", "query_cov": 1.0, "subject_cov": 0.8142857142857143, "identity": 1.0, "score": 119.0, "evalue": 4.16e-34, "valid": true, "gene": "YbcJ", "product": "Ribosome-associated protein YbcJ, S4-like RNA-binding protein", "uniref50_id": "UniRef50_N9UZE0", "cog_id": "COG2501", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_N9UZE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UZE0" ], "product": "RNA-binding S4 domain-containing protein" }, "genes": [ "ybcJ" ], "id": "DFOKDJOJIG_53", "locus": "GCA013367855_00011" }, { "type": "cds", "sequence": "contig_1", "start": 5591, "stop": 5788, "strand": "+", "frame": 2, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "COG:COG0227", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_012855288.1", "SO:0001217", "UniParc:UPI0001BC1FC5", "UniRef:UniRef100_D1J7E4", "UniRef:UniRef50_Q98PM6", "UniRef:UniRef90_B3PLS0" ], "nt": "ATGCCAGGAAGAGATCAATTAACAGGTCAAAAAGCTCTAAGTGGAAACAAAAGATCACACGCTTTAAACACCGCAAAAAGAACATTTGACTTAAATCTTCAAAAAGTTACTGTTTTAACTGAAACCGGAAGTAAAAAAACAGTTCGTGTAACTGCAAAAAATGCTAGAACACTAAAAAAATATGGTCTAGTTGCATAG", "aa": "MPGRDQLTGQKALSGNKRSHALNTAKRTFDLNLQKVTVLTETGSKKTVRVTAKNARTLKKYGLVA", "aa_hexdigest": "be79e6e221684802b48e19dfde4c6d71", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC1FC5", "ncbi_nrp_id": "WP_012855288.1", "uniref100_id": "UniRef100_D1J7E4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC1FC5", "RefSeq:WP_012855288.1", "UniRef:UniRef100_D1J7E4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7E4", "uniref90_id": "UniRef90_B3PLS0" }, "psc": { "uniref90_id": "UniRef90_B3PLS0", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q98PM6", "cog_id": "COG0227", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PM6" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "DFOKDJOJIG_54", "locus": "GCA013367855_00012" }, { "type": "cds", "sequence": "contig_1", "start": 6000, "stop": 6149, "strand": "+", "frame": 3, "gene": null, "product": "Core-binding (CB) domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q154", "UniRef:UniRef90_UPI0015885065" ], "nt": "ATGAGAAATATAGATCAATTAGAAATCTTTTATGCTGAATTTGAAACGAAGGTTCAAACACATAACAGAAGTGCTAAAACACTTAAAAATTATACATTTGTTTTAAGACAATTAAGAGACTGGGACACCCTCGAAGAATTAATAGAATAA", "aa": "MRNIDQLEIFYAEFETKVQTHNRSAKTLKNYTFVLRQLRDWDTLEELIE", "aa_hexdigest": "8d7a1549c2a488024c8e7ceaa4c59769", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0015885065", "query_cov": 0.8775510204081632, "subject_cov": 0.8431372549019608, "identity": 0.977, "score": 88.6, "evalue": 2.09e-22, "valid": true, "uniref50_id": "UniRef50_A0A6A8Q154" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q154" ], "product": "Core-binding (CB) domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_55", "locus": "GCA013367855_00013" }, { "type": "cds", "sequence": "contig_1", "start": 6185, "stop": 6352, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCAATACAAGCGTAAAACTTTATTAAATAATTTTCCAAGCAATTTAAAACAAAAAATTTCTGAATTTTTAAAAAGCAATGAACTAGATTTAAATATAAGAGCTGAAAAAATTACAAAAGAAATTTCTATTAATTTATTCAAATTTATAAACAATAAAAATTAA", "aa": "MFQYKRKTLLNNFPSNLKQKISEFLKSNELDLNIRAEKITKEISINLFKFINNKN", "aa_hexdigest": "d061f8f47610aed152b4b48fc3d66d05", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6618.659699999998, "isoelectric_point": 9.996741676330569 }, "id": "DFOKDJOJIG_56", "locus": "GCA013367855_00014" }, { "type": "cds", "sequence": "contig_1", "start": 6389, "stop": 6958, "strand": "-", "frame": 3, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "COG:COG0030", "COG:J", "EC:2.1.1.182", "GO:0000179", "GO:0006364", "SO:0001217", "UniRef:UniRef50_D1J7E5", "UniRef:UniRef90_D1J7E5" ], "nt": "ATGATTAAGGCATTGAAATCACTAGGCCAAAATTTCTTAAGCAATAAAAACATTCAAAAAGAGATTGTAAAGGTTGCGAACGTTGCAGGCAAAAATATTATTGAAATTGGCCCGGGAATGGGCGCAATAACTGATCAAATGGCAGATGTTGTAAATAGACTTGTATGTATTGAATTTGATAAAAGATTATACGAATTTTTATTACAAAAAAATTATTCAAGCAATGTTGAAATATTGAACCAAGATTTTTTACAAACAGATTTATTAAAATATACTGATTTTGAAGTAATTGGAAACATTCCTTACAATATAACAAGCGATATAATTTTTAAATTATTAGATAACGCTAAAAACATTAATAAAATAACATTAATGGTTCAAAAAGAAGTAGCAGATAGAATATGCTGTGGAGCAGGCAATTCTCAATATAGTAAATTAGCAGCATCAATGCAACTTCTCTATGAACCTAAATATTTATTCACTGTTAAGGCAAAAGAATTTAATCCCGCTCCTAAAGTAGATTCGGCAGTTATTCAACTAAATCTAATAAAGAATAATGATTTTATTTGA", "aa": "MIKALKSLGQNFLSNKNIQKEIVKVANVAGKNIIEIGPGMGAITDQMADVVNRLVCIEFDKRLYEFLLQKNYSSNVEILNQDFLQTDLLKYTDFEVIGNIPYNITSDIIFKLLDNAKNINKITLMVQKEVADRICCGAGNSQYSKLAASMQLLYEPKYLFTVKAKEFNPAPKVDSAVIQLNLIKNNDFI", "aa_hexdigest": "4b11301de4ef2f6262c6460fa1ed059d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rsmA" ], "truncated": "5-prime", "pseudogene": { "start": 6185, "stop": 6982, "observations": { "mutation": [ 6391 ], "stop": [ 6391 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E5", "query_cov": 1.0, "subject_cov": 0.7132075471698113, "identity": 0.9790000000000001, "score": 363.0, "evalue": 9.66e-126, "gene_start": 1, "gene_end": 189, "reference_start": 9, "reference_end": 197, "reference_sequence": "MLERFLKSMIKALKSLGQNFLSNKNIQKEIVKAANVAGKNIIEIGPGMGAITDQMADVVNRLVCIEFDKRLYEFLLQKNYSSNVEILNQDFLQTDLLKYTDFEVIGNIPYNITSDIIFKLLDNAENINKITLMVQKEVADRICCGAGNSQYSKLAASIQLLYEPKYLFTVKAKEFNPAPKVDSAVIQLILIKNNDFIWNNQVEILKFIKQMFQYKRKTLLNNFPSNLKQKISEFLKSNELDLNIRAEKITKEISINLFKFINNKN" }, "paralog": false, "identity": 0.9811320754716981, "score": 505.0, "evalue": 3.31e-184, "description": "Internal stop codon at 6391. Nonsense mutation around 6391.", "psc": { "uniref90_id": "UniRef90_D1J7E5", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_D1J7E5", "cog_id": "COG0030", "cog_category": "J", "go_ids": [ "GO:0000179", "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E5", "EC:2.1.1.182", "UniRef:UniRef50_D1J7E5", "COG:COG0030", "COG:J", "GO:0000179", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7E5" ], "product": "Ribosomal RNA small subunit methyltransferase A" } }, "id": "DFOKDJOJIG_57", "locus": "GCA013367855_00015" }, { "type": "cds", "sequence": "contig_1", "start": 6951, "stop": 7718, "strand": "-", "frame": 2, "gene": "tatD", "product": "YchF/TatD family DNA exonuclease", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.21.-", "KEGG:K03424", "RefSeq:WP_121120237.1", "SO:0001217", "UniParc:UPI000EAE1EE7", "UniRef:UniRef100_UPI000EAE1EE7", "UniRef:UniRef50_N9U095", "UniRef:UniRef90_A0A6A8Q279" ], "nt": "ATGAAATTTATCGATTTACACACCCACCCATCAACCGAATACTATTCAGATCCTCTTGAAATAGTTAATGAATGGCATAACCTAAATATGGAAAGGCTTTTTGTATGTGGAACAAACGAAGATGATTCGATACAAGCAATAGAACTTGCTAAAAAGGTTGATTTTGTTTATCCTGTTATTGGAATACATCCAACATTATCAAACGGTTTGCAAGATAGAGAAATTTTAAACAAGATATATGAACCAAATGTTGTTGCAATTGGGGAAGTTGGTCTTGATTATCATTATGATGATTCGCCTAGCAAAGAAATTCAAAAACAAGGATTTATTGCCCAAATAGATTTTGCAAAAGAACATAATTTACCAGTAATTATGCACATTAGAGATGCTTTAGATGATTCTTTAGATATAATAACCTTGCCTCAATATAAAGACATTAATTTTATATTTCACTCATTTAGTGGGGATCTTAGTTTTTTAAATAAATGCTTGACATATTCAAATATCTATTATTCAATAAGCGGGGTTGTTACTTTTAAAAATGCTAAGCCTTTACAAGAAGCAGTAAAAAATATTCCTTTAGATAGAATATTTTGTGAAACTGATACACCTTATTTAGCCCCAACTCCAATGAGAGGAAAACCCAACATTAGCCCTTTTGTTCAATACACATATGAATACATTGCTAATTTAAAACAAATTGATGTTGATGTTTTAGTTGAACAAATTAAAGAAAATGTTAGAAAGGTTTTTAAAATCTATGATTAA", "aa": "MKFIDLHTHPSTEYYSDPLEIVNEWHNLNMERLFVCGTNEDDSIQAIELAKKVDFVYPVIGIHPTLSNGLQDREILNKIYEPNVVAIGEVGLDYHYDDSPSKEIQKQGFIAQIDFAKEHNLPVIMHIRDALDDSLDIITLPQYKDINFIFHSFSGDLSFLNKCLTYSNIYYSISGVVTFKNAKPLQEAVKNIPLDRIFCETDTPYLAPTPMRGKPNISPFVQYTYEYIANLKQIDVDVLVEQIKENVRKVFKIYD", "aa_hexdigest": "bac7d5f306e0099455104882f8632492", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000EAE1EE7", "ncbi_nrp_id": "WP_121120237.1", "uniref100_id": "UniRef100_UPI000EAE1EE7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000EAE1EE7", "RefSeq:WP_121120237.1", "UniRef:UniRef100_UPI000EAE1EE7" ] }, "ips": { "uniref100_id": "UniRef100_UPI000EAE1EE7", "uniref90_id": "UniRef90_A0A6A8Q279" }, "psc": { "uniref90_id": "UniRef90_A0A6A8Q279", "gene": "TatD", "product": "YchF/TatD family DNA exonuclease", "ec_ids": [ "3.1.21.-" ], "uniref50_id": "UniRef50_N9U095", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424" }, "pscc": { "uniref50_id": "UniRef50_N9U095", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U095" ], "product": "TatD DNase family protein" }, "genes": [ "tatD" ], "id": "DFOKDJOJIG_58", "locus": "GCA013367855_00016" }, { "type": "cds", "sequence": "contig_1", "start": 7718, "stop": 7891, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATGTTGTTCATTGGCACATACATCATTTATTTTTGGCAAACCATTTTATTCATTCAAGGTAATTTCAGATTGTTTGGAATCTAATAATAACCTAGAATATAGGCAATCAAAAGCAATTTGGGAATTAAAAATAATTGATATTTTTAAAGATATATTAGGAGAATTATAA", "aa": "MECCSLAHTSFIFGKPFYSFKVISDCLESNNNLEYRQSKAIWELKIIDIFKDILGEL", "aa_hexdigest": "aef54c524208904dd78ed868a5e09c2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6649.642099999997, "isoelectric_point": 5.122978019714354 }, "id": "DFOKDJOJIG_59", "locus": "GCA013367855_00017" }, { "type": "cds", "sequence": "contig_1", "start": 7916, "stop": 8251, "strand": "-", "frame": 3, "gene": "mtnN", "product": "Nucleoside phosphorylase domain-containing protein", "db_xrefs": [ "COG:COG0775", "COG:FH", "SO:0001217", "UniRef:UniRef50_A0A454C9F7", "UniRef:UniRef90_A0A454C9F7" ], "nt": "TTGTTAGCACATTGTGGAGTTGGAAAAACTAATTCTGCTTTAATGTTAAGTTATTTATTGACTAAGTTCAATATATTAAAAGTAATTAACATTGGACCAGCTATATCAATTTTTAATGGTTGTGTTGGAGATATTTATTGCATACAAAACTGCAAATATTATGATGTTAATTTAACCGCCTTGCCTAATTATAAAATAGGGCAATTGCCTAGTTTGCAAGATGTATTTCATTGCAATCAAGATCTAAAAATTTGCAGCAATTTTAAAAAAGCCAATATTATAACCGGTGACAGTTTTATTAATAAAAGCATAATAAATTCAAATATATTAGATTGA", "aa": "MLAHCGVGKTNSALMLSYLLTKFNILKVINIGPAISIFNGCVGDIYCIQNCKYYDVNLTALPNYKIGQLPSLQDVFHCNQDLKICSNFKKANIITGDSFINKSIINSNILD", "aa_hexdigest": "4ee1871acabbed1982ed792a05d2a2c6", "start_type": "TTG", "rbs_motif": null, "genes": [ "mtnN" ], "truncated": "both", "pseudogene": { "start": 7718, "stop": 8404, "observations": { "mutation": [ 7918, 8269 ], "stop": [ 7918, 8269 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9F7", "query_cov": 1.0, "subject_cov": 0.4868421052631579, "identity": 0.973, "score": 221.0, "evalue": 2.35e-71, "gene_start": 1, "gene_end": 111, "reference_start": 52, "reference_end": 162, "reference_sequence": "MEKKFYLFIFAEPLETEQLRIKYSKHLKQIKIDFENNFQNLYELNWNNKQILLAHCGVGKTNSALMLSYLLTKFNILKVINIGPAISIFNGSVGDIYCIQNCKYYDVNLTALPNYKIGQLPSLQDVFHCNQDLKICSNFKKANIITGDSFINKSIINSDILDWNTNNLLGDMECCSLAHTSFIFGKPFYSFKVISDCLESNNNLEYRQSKAIWELKIIDIFKDILGEL" }, "paralog": false, "identity": 0.9824561403508771, "score": 454.0, "evalue": 5.33e-166, "description": "Internal stop codon at 7918, 8269. Nonsense mutation around 7918, 8269.", "psc": { "uniref90_id": "UniRef90_A0A454C9F7", "gene": "MtnN", "product": "Nucleoside phosphorylase domain-containing protein", "uniref50_id": "UniRef50_A0A454C9F7", "cog_id": "COG0775", "cog_category": "FH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9F7", "UniRef:UniRef50_A0A454C9F7", "COG:COG0775", "COG:FH" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9F7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F7" ], "product": "Nucleoside phosphorylase domain-containing protein" } }, "id": "DFOKDJOJIG_60", "locus": "GCA013367855_00018" }, { "type": "cds", "sequence": "contig_1", "start": 8504, "stop": 8827, "strand": "+", "frame": 2, "gene": null, "product": "nuclease-related domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00066ADC43", "UniRef:UniRef90_UPI001588B00D" ], "nt": "TTGCAAAAAGAATTTATTTTAAAATTAAAATATATGAAAATATTGTTAATCATTATTTTATGTGTTTTAATTATTCCAATTTTTATTGGCGTTCCTTGTATATTTTTTATTAAAAAACGCACAATTTTAAAAGAAAAAAACTTCAAAAAAGAATTAGGCGATAAGGGAGAACAAGAAGTAATAAAAATATTGCAAAAAATGAATGCAAAAAAATACCTAGTTTTAAACGATATTAGGTTGATGGGAGAAGGGGGCAAACGAAAACAAATAGACCATATTGTAGTATCCTCTAAAGGTATTTTTGTAATAGAAACAAAAAACTGA", "aa": "MQKEFILKLKYMKILLIIILCVLIIPIFIGVPCIFFIKKRTILKEKNFKKELGDKGEQEVIKILQKMNAKKYLVLNDIRLMGEGGKRKQIDHIVVSSKGIFVIETKN", "aa_hexdigest": "a78e2f2f1d8af23dfaea572f1cdc77dc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 8537, "stop": 9098, "observations": { "insertion": [ 9059 ], "mutation": [ 8825, 8906 ], "stop": [ 8825, 8906 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001588B00D", "query_cov": 0.897196261682243, "subject_cov": 0.5161290322580645, "identity": 1.0, "score": 177.0, "evalue": 7.98e-55, "gene_start": 12, "gene_end": 107, "reference_start": 1, "reference_end": 96, "reference_sequence": "MKILLIIILCVLIIPIFIGVPCIFFIKKRTILKEKNFKKELGDKGEQEVIKILQKMNAKKYLVLNDIRLMGEGGKRKQIDHIVVSSKGIFVIETKNWKGDIVGSKNEKYLEVVAKYSDDRVRKWSKNPIFQNKAHIEALNSILSKSINEEINYYSIIVFVDKNKIYFRNDESNDKKKSYILKKRYF" }, "paralog": false, "identity": 0.9623655913978495, "score": 343.0, "evalue": 7.1e-123, "description": "Internal stop codon at 8825, 8906. Frameshift due to insertion around 9059. Nonsense mutation around 8825, 8906.", "psc": { "uniref90_id": "UniRef90_UPI001588B00D", "product": "nuclease-related domain-containing protein", "uniref50_id": "UniRef50_UPI00066ADC43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001588B00D", "UniRef:UniRef50_UPI00066ADC43" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00066ADC43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00066ADC43" ], "product": "nuclease-related domain-containing protein" } }, "id": "DFOKDJOJIG_61", "locus": "GCA013367855_00019" }, { "type": "cds", "sequence": "contig_1", "start": 9008, "stop": 9229, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGTTAATAAGAATAAAATATATTTTAGAAATGATGAGTCGAATGATACGGAAAAAAAGTTATATTTTAAAGAAAAGATATTTTTAATACGTTTAGATGAAGTTTTAAGCACAATTTATCGTTGCATTAATAATAAAGTTTACAAAAAAACAGCAAAAAGTATAAATATTAAGTTAATAGAAAATATTATTCATAAAGCAGAAACTAAAGAAAAATAA", "aa": "MFVNKNKIYFRNDESNDTEKKLYFKEKIFLIRLDEVLSTIYRCINNKVYKKTAKSINIKLIENIIHKAETKEK", "aa_hexdigest": "e9b94d6379dcedb8959a704a8a33e17c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8858.292099999995, "isoelectric_point": 9.607481575012208 }, "id": "DFOKDJOJIG_62", "locus": "GCA013367855_00020" }, { "type": "tRNA", "sequence": "contig_1", "start": 9392, "stop": 9478, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 74.5, "nt": "GCCCAGGTGGCGGAATGGTAGACGCTACGGACTTAAAATCCGTTGGCTGTAATGGCCGTGCTGGTTCAAGTCCAGTCCTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 9425, 9427 ], "id": "DFOKDJOJIG_1", "locus": "GCA013367855_00021" }, { "type": "tRNA", "sequence": "contig_1", "start": 9484, "stop": 9559, "strand": "+", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 80.1, "nt": "GCGCCCTTAGCTCAGCAGGTAGAGCAAATGGCTTTTAACCATTGGGTCAGAGGTTCGAATCCTCTAGGGCGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 9516, 9518 ], "id": "DFOKDJOJIG_2", "locus": "GCA013367855_00022" }, { "type": "cds", "sequence": "contig_1", "start": 9797, "stop": 9982, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17676.7" ], "nt": "TTGAAACCTAATGAACTATGTGATAAGGTTTTATTTCTTGAAACAAGTGAAGAAAGGCCAAGCCCACAATTACTTAAAAAGATGTTGCTAAAATTCAAAGAAGTTGGCTTATTTAAAAATATTCAAGGAATATTAATCGGATGCCCTCAAAATCGTGTTTATTATAATGAATATAAAGAAATTTGA", "aa": "MKPNELCDKVLFLETSEERPSPQLLKKMLLKFKEVGLFKNIQGILIGCPQNRVYYNEYKEI", "aa_hexdigest": "230efee2d4604548c2217854d8e8abe5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17676.7", "name": "Peptidase_S66C", "length": 50, "aa_cov": 0.7704918032786885, "hmm_cov": 0.96, "evalue": 6.830825889613292e-10, "score": 35.27320861816406, "start": 4, "stop": 50 } ], "seq_stats": { "molecular_weight": 7218.4823000000015, "isoelectric_point": 8.617182731628418 }, "id": "DFOKDJOJIG_63", "locus": "GCA013367855_00023" }, { "type": "cds", "sequence": "contig_1", "start": 10235, "stop": 10723, "strand": "-", "frame": 3, "gene": "mccF", "product": "Microcin C7 resistance MccF related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X0PHG8" ], "nt": "ATGATCAAACCAATAAAATTAAGTAAAGAAAATGGCATTGCAATAGTAAGCTTATCTGCAGGAACTATTGGAGAAGAATTTGCTAGTCACCAATTAGATTTAGCAATAAAAAGACTCAATTTGGATTTTCAACTTAACGTTATGATTGCTAAAAACGCACTTAAAGGAATAAAAGTTTTAAAAGAAAATCCTATGTTAAAAGTTGAGGATTTAAATGAAGTTTTTAGCAATGTCAATGTAAAAGCAATAATTTGTGCAATTGGTGGATTTGACACATACAAAACTATTCCACTTTTATTAGAAGACGAAAACTTTAAAAATTTAGTCTTAAATAATCCTAAAATTTTTATTGGATATTCAAATTCAACCGTTAACCATTTAATGTTTTATAGATTAGGACTAAAAACATTTTATGGTCACAACATTTTATGCGATATTGCTGAACTTGACAAAGAAATGTTGCCTTATACTAAAAACTCATTTAAATGA", "aa": "MIKPIKLSKENGIAIVSLSAGTIGEEFASHQLDLAIKRLNLDFQLNVMIAKNALKGIKVLKENPMLKVEDLNEVFSNVNVKAIICAIGGFDTYKTIPLLLEDENFKNLVLNNPKIFIGYSNSTVNHLMFYRLGLKTFYGHNILCDIAELDKEMLPYTKNSFK", "aa_hexdigest": "0b3f3e31ac7d889334cd1f3332f3587f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00308136E8", "query_cov": 0.9938271604938271, "subject_cov": 1.00625, "identity": 0.547, "score": 182.0, "evalue": 4.6e-56, "valid": false, "product": "LD-carboxypeptidase", "uniref50_id": "UniRef50_X0PHG8" }, "pscc": { "uniref50_id": "UniRef50_X0PHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X0PHG8" ], "product": "Microcin C7 resistance MccF related protein" }, "genes": [ "mccF" ], "id": "DFOKDJOJIG_64", "locus": "GCA013367855_00024" }, { "type": "cds", "sequence": "contig_1", "start": 10814, "stop": 11464, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGAAACTAATGAAATATTTATTGACCCTGATCAAATAGAAAAAAATAATGTTAAACTAAACAAAACAGAAAATTATGCATATAAAGATTTAAACGATAGACTTCATCATTTTGATTCAAATCCCGAAACATTATTCATTAACGAAGCAAAGAAAATAATTAGTGAATATAAAAATGAAATAAACGTCAAAGTTTATACAAAAAACCCTGTTTTTCATGGTTTAAAATTAGAATATTTTGATGAAGAAAGAGAAATTAAGTCTTCATATCCTGATTTTGTTTTTAAAATAAATAGTGGTGAAGTAACAAATTATTTATATATTGAAGTTAAAAGTGCAAAACACGATTATAATCAACAAAAAACTAAAAACATAGTTACTAGCTATAAAAAATATTTAGATAATAAAAAACCTGATAATGCTTTATTTGTTGATGACGTAGAAAAAAATAATAATTACACGTTTTTAATTTGTTATGTTGATGAAGAAAACCAAGACTTTTATTTTTTAGGGGCAAGTGATAATGCAGAACTTGATAAAGAGGTCAATATATCTATTCCTGACGGCATAAAAGAATTAATTATCAATAAACATAAATTTATTTCAGATATTGAATCATTATTCAAATATTTTCAAAACAATAAATAA", "aa": "MPETNEIFIDPDQIEKNNVKLNKTENYAYKDLNDRLHHFDSNPETLFINEAKKIISEYKNEINVKVYTKNPVFHGLKLEYFDEEREIKSSYPDFVFKINSGEVTNYLYIEVKSAKHDYNQQKTKNIVTSYKKYLDNKKPDNALFVDDVEKNNNYTFLICYVDEENQDFYFLGASDNAELDKEVNISIPDGIKELIINKHKFISDIESLFKYFQNNK", "aa_hexdigest": "2a82360edd4ecbfc2f2a8470af0ec730", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25634.31950000003, "isoelectric_point": 5.0784730911254865 }, "id": "DFOKDJOJIG_65", "locus": "GCA013367855_00025" }, { "type": "cds", "sequence": "contig_1", "start": 11723, "stop": 12124, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGTGCAACTTAGAAGAGTGTCATCAAGTAGTTTGTCTATTCAAACAATAGGAAGAATTAAAAGATTCCCAAATCCAAGTTATAACAAAGATCAAGTTTCTAGAGAAGGCAATAGATACTATATTTATTCAAATGCTCAAACCAAAGAAAATATAAGGAGAACATTGATTTTAAAACGAGAATTTGAAAATGAAAAATTTGTTTATGGCCAAATAAATAAAGTAAAAATCAAACAAGCAATAATAGGAGCAGATTTTATTAGAGAAGCTAAAAATTCTATTGTCAAAGAAAAAATTGATGAATATTTTAAAAAATACAGTAAAGAATATATTAAAAACAACAAGAAATTAATAGGAGAACAAGGAAACATTGAAGATAAAATTAGAGTTTATTCATGA", "aa": "MLVQLRRVSSSSLSIQTIGRIKRFPNPSYNKDQVSREGNRYYIYSNAQTKENIRRTLILKREFENEKFVYGQINKVKIKQAIIGADFIREAKNSIVKEKIDEYFKKYSKEYIKNNKKLIGEQGNIEDKIRVYS", "aa_hexdigest": "ab980c984337dfad5b791589aad3c64f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15789.973399999988, "isoelectric_point": 9.961477470397949 }, "id": "DFOKDJOJIG_66", "locus": "GCA013367855_00026" }, { "type": "cds", "sequence": "contig_1", "start": 12143, "stop": 13285, "strand": "-", "frame": 3, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2BAF28" ], "nt": "TTGAAAGAAAAGATAGCAATGAATCTAACTAGTGTACAAGAAAAAGCAGTTAAACAATTGCTTGAAGAATATAAAATTGCAATTCAATCTAGCGAACCTAAAATAGTTGATTTTCAAGCTCCAACTGGTAGCGGTAAAACCTTTATGTTGGCCAACACTATAAATGAAATAATAAAAGAAAATAATGCCAATGGTGAACCAAACAAATTAGTCTTTGTTATTGCTACACTTTCATCAGCAGATTTGCCTTATCAAATGGAATCAAACCTAAATGAATATAAAAGATATATTAATGGTTTGTATGAAGTAGAAAGAAAGGAATCTCCTTCCTTGAATTCTCAAAAAAATAAAGATTCAACTTATAATTTAATTCCCCAAAAAAATAAAGTAATAATCTTCGGTGTTTCATCATTTGGTAAAAATAGAATATTTACCGAACAAGGTTTGCTTGAAAGCTTTCTTGATCAAATTAAATATCAAAACTATACATTAGTTTATATTAGAGATGAAGCACATCATGGTGGAAATATAAAAGGTAGAAGTAATTTTAAATTTGAAGATATTGATACAATCCATGATGAATCTAAATTAAAAAAAGAAGAAGTTAGATTTGAATATAAAATTCAAAAAGCAGCCAATTTAATAATAAAAATGACAGCAACTCCTAAGGGAACACATAAACTTGTCTATATTGATGAAAAGGATTTAGCCGAAGATGATATTAAATTGCTAAAAGATGAATTAATTTATAACAAGGGTATAACTGACTCAAGTCAAGATTTAATTGATGATAATGAATTACTAAATCAAGCATGTGAAACATTTAAAGAAGTTCAAAGCCAATATGCAAACAATAATAGTGATTTAAAAAATATAAGACCAGCAATGCTTATTCAAGTTGAAAATGAACCAAGTGAAAAATCAACTGCCAAAGAAAAAGATGATTTTAAAGAAAGCATTGAGAACATAATTAAAAAATTAAATGAATGTAATTTGAGTTATGTTAAATACTTTTCAAATGATAAAGTGAAATCTTCTTCTTTAATAAAGGAAGAAAATCACAAAATAAACTTAAAAGAAATTTCAAAAAATGACTCTAATGTTGATGCAATAATTTTTAAGATTGGACCTTCAACTGGTTGA", "aa": "MKEKIAMNLTSVQEKAVKQLLEEYKIAIQSSEPKIVDFQAPTGSGKTFMLANTINEIIKENNANGEPNKLVFVIATLSSADLPYQMESNLNEYKRYINGLYEVERKESPSLNSQKNKDSTYNLIPQKNKVIIFGVSSFGKNRIFTEQGLLESFLDQIKYQNYTLVYIRDEAHHGGNIKGRSNFKFEDIDTIHDESKLKKEEVRFEYKIQKAANLIIKMTATPKGTHKLVYIDEKDLAEDDIKLLKDELIYNKGITDSSQDLIDDNELLNQACETFKEVQSQYANNNSDLKNIRPAMLIQVENEPSEKSTAKEKDDFKESIENIIKKLNECNLSYVKYFSNDKVKSSSLIKEENHKINLKEISKNDSNVDAIIFKIGPSTG", "aa_hexdigest": "1fb92dc3a6137cc69378e6c9f3b88bb6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001981E5C", "query_cov": 0.8763157894736842, "subject_cov": 1.021472392638037, "identity": 0.595, "score": 363.0, "evalue": 1.2e-121, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_UPI001E2BAF28" }, "pscc": { "uniref50_id": "UniRef50_UPI001E2BAF28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2BAF28" ], "product": "DEAD/DEAH box helicase family protein" }, "genes": [], "id": "DFOKDJOJIG_67", "locus": "GCA013367855_00027" }, { "type": "cds", "sequence": "contig_1", "start": 13254, "stop": 13442, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAAAATTCAATATTATGATACTGATGCGTTTGCCAATAACAACGAAGCAATTAAAAGCGATTTTATAAAAATGCTTAATGATTTTGAAGTTTCAAAAATTAACAATATAAACGAACAAGAAATTTTATTAAATTTAACTGCTTTAAAACCAGTTGAAAGAAAAGATAGCAATGAATCTAACTAG", "aa": "MFKIQYYDTDAFANNNEAIKSDFIKMLNDFEVSKINNINEQEILLNLTALKPVERKDSNESN", "aa_hexdigest": "cce6f69c2cd525ca4557b47692ee6e8e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7243.0131999999985, "isoelectric_point": 4.540378379821779 }, "id": "DFOKDJOJIG_68", "locus": "GCA013367855_00028" }, { "type": "cds", "sequence": "contig_1", "start": 13479, "stop": 13856, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01555.25" ], "nt": "TTGGTAGATAATGATAATAAACCAGTTCAAAGAACATCAAAATTTTCTAATTTGCTATATTCATTCCATGGTTCTTTGGGTACGGCACTTCAAAAAGAAATTTTTAATTTAAAAGTTTTTGACCATCCCAAACCAATCGAACTAATTAAGTATTTAATTAACATTCATCCTAATCCTGATGCTCGCATTCTTGACTTTTTTGCAGGTTCAGGAACAACCGGACATGCAGTTTTAGATCTTAATGGAGAAGATGGGGGCAATAGAACCTTCACATTAGTTACAAATAATGAAAATAATATTGGTATAAATGTTTGCTATGAAAGGCTTTATAGAATTAATAAAGGCAGTGGAAGTAAAGGTGAAACCGACTTTGATTGA", "aa": "MVDNDNKPVQRTSKFSNLLYSFHGSLGTALQKEIFNLKVFDHPKPIELIKYLINIHPNPDARILDFFAGSGTTGHAVLDLNGEDGGNRTFTLVTNNENNIGINVCYERLYRINKGSGSKGETDFD", "aa_hexdigest": "bad4dd653a2dfc2b70fb53c99687f046", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01555.25", "name": "N6_N4_Mtase", "length": 56, "aa_cov": 0.448, "hmm_cov": 0.8392857142857143, "evalue": 2.635908605147766e-13, "score": 46.03828430175781, "start": 28, "stop": 83 } ], "seq_stats": { "molecular_weight": 13945.437999999978, "isoelectric_point": 6.050817680358887 }, "id": "DFOKDJOJIG_69", "locus": "GCA013367855_00029" }, { "type": "cds", "sequence": "contig_1", "start": 14061, "stop": 14204, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCAAAAAATATTGAACATATGAATACAAATAATTATTCAAAAAACATTGATGATGGTTCATACAAATTAAGAGATGAATATTTTGAAACCAGAGGTATGTATAAACTTAAACAACTTGATTTTGGATCTTTAACTTGA", "aa": "MYAKNIEHMNTNNYSKNIDDGSYKLRDEYFETRGMYKLKQLDFGSLT", "aa_hexdigest": "ecfd8266086b18defeac5555c45da795", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5644.2439, "isoelectric_point": 6.532584953308105 }, "id": "DFOKDJOJIG_70", "locus": "GCA013367855_00030" }, { "type": "cds", "sequence": "contig_1", "start": 14271, "stop": 14420, "strand": "-", "frame": 2, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025CFB7FC" ], "nt": "ATGATGAACGAAAGATTAAAAATGGCTCGCCAACTTTTAAAAGAAGATGGCGTTATTTTTGTTTCAATTGACGACAATGAACAAGCATATTTAAAGGTGTTAATGGATGAAATTTTTGGCGAAGAAAATTTTGTATGTAATTTTGTGTGA", "aa": "MMNERLKMARQLLKEDGVIFVSIDDNEQAYLKVLMDEIFGEENFVCNFV", "aa_hexdigest": "2a496749000845df1e1b5fc2aaa2b852", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0025CFB7FC", "query_cov": 0.8979591836734694, "subject_cov": 0.88, "identity": 0.727, "score": 68.6, "evalue": 1.78e-14, "valid": false, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0025CFB7FC" }, "pscc": { "uniref50_id": "UniRef50_UPI0025CFB7FC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025CFB7FC" ], "product": "DNA methyltransferase" }, "genes": [], "id": "DFOKDJOJIG_71", "locus": "GCA013367855_00031" }, { "type": "cds", "sequence": "contig_1", "start": 14549, "stop": 14923, "strand": "-", "frame": 3, "gene": null, "product": "Pseudogen of Type III restriction modification system: methylase (Part 1)", "db_xrefs": [ "RefSeq:WP_241862864.1", "SO:0001217", "UniParc:UPI001F5202EB", "UniRef:UniRef100_UPI001F5202EB", "UniRef:UniRef50_D1J7F1", "UniRef:UniRef90_D1J7F1" ], "nt": "ATGAAGGATTTAAAAAACTATATAGATGATATAGATAACTTGAATTCTTTTTTATTAAATCAAGACCAAAAGAAATTAATAAAAGAAATTTTAGTTAACACAAACAATTCAAATAAAGAAAACTTAGACAATGTTTTCCAACTTTTGATTCAAAGAGTTAAAACAGGCTTTGTTTTTGATGCTGCACCTAGTGTTGATACAAAACAAATTGCAATATTAAAAAAGCAAGAAAAATTATCATTTACAAATGATTTATTGAATACTAATAAAAACACTTTAATTATTGGTGAAAATTATGATGCATTAAAAAACCTACTTGCAATTGAAAGACAAAAAGAACAAATAGGGGGGGGGGGGATTATAATTACGATTTAA", "aa": "MKDLKNYIDDIDNLNSFLLNQDQKKLIKEILVNTNNSNKENLDNVFQLLIQRVKTGFVFDAAPSVDTKQIAILKKQEKLSFTNDLLNTNKNTLIIGENYDALKNLLAIERQKEQIGGGGIIITI", "aa_hexdigest": "09866b1339f79f43d0fd268d3fc500b3", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI001F5202EB", "ncbi_nrp_id": "WP_241862864.1", "uniref100_id": "UniRef100_UPI001F5202EB", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F5202EB", "RefSeq:WP_241862864.1", "UniRef:UniRef100_UPI001F5202EB" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F5202EB", "uniref90_id": "UniRef90_D1J7F1" }, "psc": { "uniref90_id": "UniRef90_D1J7F1", "product": "Pseudogen of Type III restriction modification system: methylase (Part 1)", "uniref50_id": "UniRef50_D1J7F1" }, "pscc": { "uniref50_id": "UniRef50_D1J7F1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7F1" ], "product": "Pseudogen of Type III restriction modification system: methylase (Part 1)" }, "genes": [], "id": "DFOKDJOJIG_72", "locus": "GCA013367855_00032" }, { "type": "cds", "sequence": "contig_1", "start": 15232, "stop": 15321, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACAAAGAAATAGATGCTAAACAAGCAAAACAAGAAAGAGCAGAAAAATTATTTGGTCAAACACCAATAAAAAAAGCAATTTGA", "aa": "MKNKEIDAKQAKQERAEKLFGQTPIKKAI", "aa_hexdigest": "64c0078cc5b697f9a98ccb8247f59a16", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3342.9089000000004, "isoelectric_point": 9.873542594909669 }, "id": "DFOKDJOJIG_73", "locus": "GCA013367855_00033" }, { "type": "cds", "sequence": "contig_1", "start": 15355, "stop": 15774, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FC234F" ], "nt": "ATGGCCGGGCTATATGGATTTATTGACCAAATTTTTATTTTGAATTTGGTTCCAAAAAATAGAAACATCTTTGGAAATAATGAAAGTGAAATTGTTCAATTTTTAAATCCTGCCTTGCATAGTGGTGGATTTTTTAGTGATTATAAGATAATGATTGAACAATATAATTTACATTCAATTACGAAATTAGCTGAAATTACTCCTAATAGTATTGTTTCAACTGCGTCAGCAGTATTTGGCCCATTAATATTATTTTCTAATGCAATAGTATTTTTAGTACCTGTTGGAGCTTCAATTTATTACACTAAATGTGTAAGTAAGCAACTAAAACGGGCTGGCCAAGATTTATGGGCGACTATGTTTTGGTTTACGGTTGCGCTTTCTTTATTTGCTACGTTAGTATCTTTTGTGTTTATATGA", "aa": "MAGLYGFIDQIFILNLVPKNRNIFGNNESEIVQFLNPALHSGGFFSDYKIMIEQYNLHSITKLAEITPNSIVSTASAVFGPLILFSNAIVFLVPVGASIYYTKCVSKQLKRAGQDLWATMFWFTVALSLFATLVSFVFI", "aa_hexdigest": "18c33e03fb1f6f68af633214941e2da1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002FC234F", "query_cov": 0.9856115107913669, "subject_cov": 0.8154761904761905, "identity": 0.569, "score": 152.0, "evalue": 1.72e-44, "valid": false, "uniref50_id": "UniRef50_UPI0002FC234F" }, "pscc": { "uniref50_id": "UniRef50_UPI0002FC234F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FC234F" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15496.946199999986, "isoelectric_point": 7.86406307220459 }, "id": "DFOKDJOJIG_74", "locus": "GCA013367855_00034" }, { "type": "cds", "sequence": "contig_1", "start": 16615, "stop": 16767, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGCTTAGAATAATTGCCGTTTCATTTGTTGTTGTTTCATTGCTAGCATTTCAAGGTTCAAATGATATTGCAAAGTCTATCTTTGCCTCAATATTTGAAAACTTTATTTGCTTCGTAATAATTGTTCCTATTGGATATGGAGTTGCTTGA", "aa": "MSLRIIAVSFVVVSLLAFQGSNDIAKSIFASIFENFICFVIIVPIGYGVA", "aa_hexdigest": "73582e608da4e58ea5cf9f34c5d41445", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5381.394599999998, "isoelectric_point": 5.820676612854004 }, "id": "DFOKDJOJIG_75", "locus": "GCA013367855_00035" }, { "type": "cds", "sequence": "contig_1", "start": 17120, "stop": 17581, "strand": "-", "frame": 3, "gene": null, "product": "DUF996 domain-containing protein", "db_xrefs": [ "RefSeq:WP_047207547.1", "SO:0001217", "UniParc:UPI000640192E", "UniRef:UniRef100_UPI000640192E", "UniRef:UniRef50_A0A454C8X1", "UniRef:UniRef90_D1J7F6" ], "nt": "ATGGAAAATAATTCAAAAAACTTGAAAACCAAAGAAAATGCAAAACTTCTAATAATATTTTCAGCAATCTTATTAGGAATAGATGTTATTTATATTATTTGTGGTTCGCTTGTACCAGTATTTGGATTATTATTAGCAATAGCAAGCATCGTCTTTTTTATTCTAACAGTTGTTTATGGATTTAAAACAGGATCTCGCGGCTTAAGTAAAAATGAAATAACAACAAGAAAATTATCAATTTCGTTAGTTGCCTTGTTTATAATTTCATTCGTCTTAGCAATTGTTGCTACAGCAATGTTTTTATCAGGTGCTGCTAAAACTGCCATAGCAATATATGGACTTTCAGTAATTGCTAATCTTGCACAATTAGGTGTTTTAATTGCATTATTATGTTTTGCAGTAAAAACTTATAATAGTAAAAACAATTCACACCCAGCCCAACAACAAAATTTAAGACAATAA", "aa": "MENNSKNLKTKENAKLLIIFSAILLGIDVIYIICGSLVPVFGLLLAIASIVFFILTVVYGFKTGSRGLSKNEITTRKLSISLVALFIISFVLAIVATAMFLSGAAKTAIAIYGLSVIANLAQLGVLIALLCFAVKTYNSKNNSHPAQQQNLRQ", "aa_hexdigest": "14444f6586ee2263f6b38f2740978bc1", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000640192E", "ncbi_nrp_id": "WP_047207547.1", "uniref100_id": "UniRef100_UPI000640192E", "db_xrefs": [ "SO:0001217", "UniParc:UPI000640192E", "RefSeq:WP_047207547.1", "UniRef:UniRef100_UPI000640192E" ] }, "ips": { "uniref100_id": "UniRef100_UPI000640192E", "uniref90_id": "UniRef90_D1J7F6" }, "psc": { "uniref90_id": "UniRef90_D1J7F6", "uniref50_id": "UniRef50_A0A454C8X1" }, "pscc": { "uniref50_id": "UniRef50_A0A454C8X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1" ], "product": "DUF996 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_76", "locus": "GCA013367855_00036" }, { "type": "cds", "sequence": "contig_1", "start": 17763, "stop": 18296, "strand": "-", "frame": 2, "gene": null, "product": "DUF996 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1", "UniRef:UniRef90_A0A454C8X1" ], "nt": "GTGAATAATAACTTAAAAACTAAAGAAAATGCAAAACTTCTAATAATATTTTCATCAATCTTATTAGGAATAGATGTTATTTATATTATTAGTGGTTCGCTTGTGCCAATATTTGGATTATTATTAGCAATAGCAAGCATTGTGTTTTTTATTTTAACAGTTGTTTATGGATTTAAAACAGGATCACGTGAACCAAAAAATAGTAATCGAATGATAATAAGAAAATTATCCATTGCATTAATAGCATTGTTTGCAGCAGCAATTGCAATGGTTATTGTTGCAATAATAATAGCAATATCTTTATCACTTGGCGCATATAATTATGAATACCCAACAAATGAATATAATCCATTTTTATTAGAGTCATCAACATTTGCACTATATATTATTTGTTTAATTTTGATCCTTGCAGAATTAGGCGTTTTAATTGCATTATTATGTTTTGCAGTTAAGGTCTATTGCGCTAAAAATAACAATAATGTTAATAATGAAACAAATAACTATGATGGAAGCTCTCAAGGCCCCCAAAATTGA", "aa": "MNNNLKTKENAKLLIIFSSILLGIDVIYIISGSLVPIFGLLLAIASIVFFILTVVYGFKTGSREPKNSNRMIIRKLSIALIALFAAAIAMVIVAIIIAISLSLGAYNYEYPTNEYNPFLLESSTFALYIICLILILAELGVLIALLCFAVKVYCAKNNNNVNNETNNYDGSSQGPQN", "aa_hexdigest": "f77eeb90fedf167478bd514dc3bc30d2", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A454C8X1", "query_cov": 0.9943502824858758, "subject_cov": 0.9411764705882353, "identity": 0.972, "score": 279.0, "evalue": 9.05e-94, "valid": true, "product": "DUF996 domain-containing protein", "uniref50_id": "UniRef50_A0A454C8X1" }, "pscc": { "uniref50_id": "UniRef50_A0A454C8X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1" ], "product": "DUF996 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_77", "locus": "GCA013367855_00037" }, { "type": "cds", "sequence": "contig_1", "start": 18422, "stop": 19306, "strand": "-", "frame": 3, "gene": "clpB", "product": "Chaperone ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_D1J7F8" ], "nt": "TTGTCTGAAACATTAAAGAAGAGAGTGAAAGGTCAAGATCAAGCAATTGAACTAATCTCTGATGCAATCAAGCGTTCTAAAGCAAACATCAATGACCCAAATAGACCAATTGGTTCGTTCCTATTTCTAGGGCCAACTGGTGTTGGTAAAACTGAACTTGCAAAAAGTTTAGCAGAAGCTTTATTTGATGATGAAAATCACATTGTAAGATTAGACATGTCTGAATATATGGAAAAACACAGCGTGTCAAAATTAATTGGATCGCCTCCAGGTTATATTGGTTATGATAATGGGGGACAACTAACTGAAAAAATTAGACAAAATCCTTACTCAATAGTTTTATTTGATGAAATTGAAAAAGCAAATTCAGATGTTTTAAATATATTGTTACAAATATTGGATAATGGTACCTTAACAGATAGTACAGGAAGGGTAGTAAACTTTAAAAACACAATAATTATCATGACTTCAAACATTGGAAGTAGTGAAATTATAAATAAGAGTTTAACCATTGATGGGGTAAGGGCATTATTATTAAGAAGTTTTAGACCAGAATTTATTAATAGAATCGATGAAATAGTGCCATTCAACCCATTAAGTCCTGCCGTTGTTTGCGATATTGTAAAACTAGAATTATCAAAATTAAAGAAAAGAGTTATTAATAACCATAATTTAGTAATTAATTTTGATGACAAAATAGTTAAATTCGTTGCTGATAACGCTTACGATTCCGCTTTTGGCGCTCGTCCAATTAAAAGATATATTCAAAAAAACATTGAAAACAAATTAGCAATGGAAATCATAAAAGGAAATTTAGTTGAAGGTGACGTTGCCTTATTAACAGTTTCAAATAATAATGTTATTGAATTACAAACAAATAATTAA", "aa": "MSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN", "aa_hexdigest": "28a3a307c9f5b3ecd2df9033bc508995", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_U4KPN6", "query_cov": 0.9829931972789115, "subject_cov": 0.8376811594202899, "identity": 0.5539999999999999, "score": 315.0, "evalue": 1.22e-103, "valid": false, "product": "ATP-dependent Clp protease ATP-binding subunit, 2", "uniref50_id": "UniRef50_A0A356L436" }, "genes": [ "clpA", "clpB" ], "truncated": "3-prime", "pseudogene": { "start": 18422, "stop": 19606, "observations": { "mutation": [ 19363 ], "stop": [ 19363 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F8", "query_cov": 1.0, "subject_cov": 0.4106145251396648, "identity": 0.997, "score": 563.0, "evalue": 2.76e-196, "gene_start": 1, "gene_end": 294, "reference_start": 423, "reference_end": 716, "reference_sequence": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSKWTNIPVNRLLENQKQKILNLSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN" }, "paralog": false, "identity": 0.9974619289340102, "score": 754.0, "evalue": 1.28e-273, "description": "Internal stop codon at 19363. Nonsense mutation around 19363.", "psc": { "uniref90_id": "UniRef90_D1J7F8", "gene": "ClpA", "product": "Chaperone ClpB", "uniref50_id": "UniRef50_I5D648", "cog_id": "COG0542", "cog_category": "O", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F8", "UniRef:UniRef50_I5D648", "COG:COG0542", "COG:O" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "DFOKDJOJIG_78", "locus": "GCA013367855_00038" }, { "type": "cds", "sequence": "contig_1", "start": 19361, "stop": 19549, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAGAAAATTGCCTTTACTACAAAGTGAAGGAAAATATGTTGAAGCTTCTAAAATTATGTATGTTTTAGTTCCTGAATTAATTAAAACTAGAGATGAATTGGAACAAAAAATACAATCAAGGAAAAATCGTTTAATCAAGGAAGTTGTTGATGCTGAAGAAGTTGCAGATATTGTTTCAAAATGA", "aa": "MNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSK", "aa_hexdigest": "4f04605a8c1cc55c6ed12ccb189b210b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7213.378600000001, "isoelectric_point": 7.986101722717286 }, "id": "DFOKDJOJIG_79", "locus": "GCA013367855_00039" }, { "type": "cds", "sequence": "contig_1", "start": 19607, "stop": 20572, "strand": "-", "frame": 3, "gene": "clpB", "product": "Chaperone ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_D1J7F8" ], "nt": "ATGGAAGCAAGTTATACACCTTATGAACAAGAAAAAGATCCCTTGAAGGAATTTGGTCGAAACTTAACTGAGTTAGCTACGCAGAACAAACTAGAACCTGTTATAAACCGTGATGAAGAAATAAGATCACTAGTTAGAATTTTGTCAAGAAAAACTAAAAATAATCCCGTTTTAGTTGGCGAACCCGGCGTAGGTAAAACAGCAATTGTTGAAGGCCTAGCAAGGAAAATTGTTGAAAATCAAGTTCCAGAAAACCTAAAAGGTAAACAATTAATTGAAATTGATTTACCTGCATTGGTTGCAGGAACACAATATCGTGGTCAATTTGAAGAAAGACTAAAAAAAGTATTGAAAAAGATTGATGAAGCAAATGGTGAAATCATTCTTTTCATTGATGAAATTCATATGATAATTGGCGCCGGAGGAACTGGCGATAGCAATATGGATGCGGCGAATATATTGAAACCTATGATGGCAAGAGGACAATTGCATCTAATTAGGGCAACAACATTGGATGAATATAGAAAATATATTGAAAAAGATCCAGCCCTTGAAAGAAGAATGCAAAAGATTTTAATTTCAGAACCTTCAATTGAAGATACCATTACTATATTGCGTGGTATAAAAGAAAGATTTGAAGCATATCATGAAGTTAAAATTGATGACTCTGCTATAGTTTCGGCAGCAAATTTATCAGCAAGATATATTGCAGATAGATTTTTGCCTGATAAAGCAATAGATTTAGTTGATGAAGCAGCATCAAATATAAAAACTGAAATGAACTATTTGCCTGAACCTCTTGAAAAAATTAATTATCAAATTGCTAGATTAGAAATTGAAAAAGCTGCTCTAACAAATGATAGTGAAACAAAGCAAACAGCCATTAAAAATAAAGAAAGAATTTTAGAAATAGATAAAGAACTAAATACTTTAAAATTGCAAAAAGTTGACCTTGAAAAGCGCTGA", "aa": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIRATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLVDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKR", "aa_hexdigest": "ae304a792c58dc31f4b23fb90940e8cc", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_U4KPM2", "query_cov": 0.8722741433021807, "subject_cov": 0.9621993127147767, "identity": 0.614, "score": 337.0, "evalue": 1.24e-112, "valid": false, "product": "ATP-dependent Clp protease (ATP-binding subunit, clpB), 1", "uniref50_id": "UniRef50_A0A930RWV4" }, "genes": [ "clpA", "clpB" ], "truncated": "5-prime", "pseudogene": { "start": 19304, "stop": 20572, "observations": { "mutation": [ 19363, 19609 ], "stop": [ 19363, 19609 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F8", "query_cov": 1.0, "subject_cov": 0.4483240223463687, "identity": 0.9940000000000001, "score": 604.0, "evalue": 1.01e-211, "gene_start": 1, "gene_end": 321, "reference_start": 1, "reference_end": 321, "reference_sequence": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSKWTNIPVNRLLENQKQKILNLSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN" }, "paralog": false, "identity": 0.990521327014218, "score": 797.0, "evalue": 3.59e-290, "description": "Internal stop codon at 19363, 19609. Nonsense mutation around 19363, 19609.", "psc": { "uniref90_id": "UniRef90_D1J7F8", "gene": "ClpA", "product": "Chaperone ClpB", "uniref50_id": "UniRef50_I5D648", "cog_id": "COG0542", "cog_category": "O", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F8", "UniRef:UniRef50_I5D648", "COG:COG0542", "COG:O" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "DFOKDJOJIG_80", "locus": "GCA013367855_00040" }, { "type": "cds", "sequence": "contig_1", "start": 21250, "stop": 21483, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAATTTATTGCCATGGGTTTCAGAAATAAATTCATCACTGTTTTTTATTATACTTTCAACCATTAATACAACTGTATTAGGTGCTTTATCAACTTTTTGTCTTTTTATTGCTACAAAAATTAGTATTGAAATAATAAAAATTAAAGCAACTAAAATAATTAAAGTAAATAATTGGTTGTCATTGAAACTGCTTACTTCGCCAGGATTAACTGGACCACCAAATCAATGA", "aa": "MSNLLPWVSEINSSLFFIILSTINTTVLGALSTFCLFIATKISIEIIKIKATKIIKVNNWLSLKLLTSPGLTGPPNQ", "aa_hexdigest": "3a7ae195b9c7dd9ef93ba2e382f97518", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8432.02649999999, "isoelectric_point": 9.748022651672361 }, "id": "DFOKDJOJIG_81", "locus": "GCA013367855_00041" }, { "type": "cds", "sequence": "contig_1", "start": 21493, "stop": 21900, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTATCAAAAATATCAAAATATAAATTAAATGCTGGCAAGATCAATAACCCAATAGGGTTTAACTCAGCTGAAGGTGATTCTAAGTTAGCGCCAATAATTTTGGTTCAAATATTATTTAAGAATAAACTTACTAGTGCAACCAATACAACTCCTCCAAGTATGTTTCCATACATACGGAAAGTTAAACTTATTAATGGTGAAAAGTTTGATGATGCTTCTAAGGGATTAAGAATTTTAAATAAATACTTAAATTTTTTATATATGAATCCAATGATGTGTGTTCCAAGTCAACTTATAACAGTGGCAGCAAAAATTGCTGACAAACTAGTTCCAATTGGGGCTAAACCAACTAATGAAAGCATATTTCCTAAAATCAAGAAGGCAAATAATGAAATAAAATAA", "aa": "MKLSKISKYKLNAGKINNPIGFNSAEGDSKLAPIILVQILFKNKLTSATNTTPPSMFPYIRKVKLINGEKFDDASKGLRILNKYLNFLYMNPMMCVPSQLITVAAKIADKLVPIGAKPTNESIFPKIKKANNEIK", "aa_hexdigest": "cd316ae9f96adac2d24ff4871f142a36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14983.714699999984, "isoelectric_point": 10.005122566223147 }, "id": "DFOKDJOJIG_82", "locus": "GCA013367855_00042" }, { "type": "cds", "sequence": "contig_1", "start": 22098, "stop": 22403, "strand": "+", "frame": 3, "gene": "atpE", "product": "ATP synthase F0 subunit C", "db_xrefs": [ "COG:C", "COG:COG0636", "EC:7.1.2.2", "GO:0015078", "GO:0015986", "GO:0045263", "RefSeq:WP_036433167.1", "SO:0001217", "UniParc:UPI000512B8A3", "UniRef:UniRef100_UPI000512B8A3", "UniRef:UniRef50_B3PLV3", "UniRef:UniRef90_D1J7G1" ], "nt": "ATGGATAAATTAGAAAATATTGTTAATGCATTCAATACAGCATCAGCAGGTGTTAATAGTAAAGAAGCAGGAGTAAACTACCCTCTAGCATATGGTTTAACAATGTTAGGAGCAGGTATTGCTGTTCTTGGCTCAGGTTTAGTTTCTATTGGTCAAGGTTTAGCCGTTGCTAAAGCCTGTGAAGCTGTTGGAAGAAATCCAGAAGCATTTTCAAAAATTCGTACATTGTTAATTTTGGGTTTAGGTATTGTTGAAACCGGATCTATTTATTGTTTAGTTATTTCATTATTGCTAATCTTCGTTTAA", "aa": "MDKLENIVNAFNTASAGVNSKEAGVNYPLAYGLTMLGAGIAVLGSGLVSIGQGLAVAKACEAVGRNPEAFSKIRTLLILGLGIVETGSIYCLVISLLLIFV", "aa_hexdigest": "21d84cdd637c46775f7a25fafd278151", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000512B8A3", "ncbi_nrp_id": "WP_036433167.1", "uniref100_id": "UniRef100_UPI000512B8A3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512B8A3", "RefSeq:WP_036433167.1", "UniRef:UniRef100_UPI000512B8A3" ] }, "ips": { "uniref100_id": "UniRef100_UPI000512B8A3", "uniref90_id": "UniRef90_D1J7G1" }, "psc": { "uniref90_id": "UniRef90_D1J7G1", "gene": "atpE", "product": "ATP synthase F0 subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_B3PLV3", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0015078", "GO:0015986", "GO:0045263" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLV3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLV3" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "DFOKDJOJIG_83", "locus": "GCA013367855_00043" }, { "type": "cds", "sequence": "contig_1", "start": 22408, "stop": 22533, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCAAAATTTTGCAAATTTATTAAGCCAATCGCAAAATGCAATTGCAGCAGCTAAGCCATCTGCTTCTGCTAAAATTGCAGAAGAACTAAACAAAACTTTTAATGGTTTAACATTCAATTGA", "aa": "MFQNFANLLSQSQNAIAAAKPSASAKIAEELNKTFNGLTFN", "aa_hexdigest": "14512e228ffe99de195f5f6ed3e87fe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4401.906899999999, "isoelectric_point": 8.254934883117677 }, "id": "DFOKDJOJIG_84", "locus": "GCA013367855_00044" }, { "type": "cds", "sequence": "contig_1", "start": 22576, "stop": 22989, "strand": "+", "frame": 1, "gene": "atpF", "product": "ATP synthase subunit b", "db_xrefs": [ "COG:C", "COG:COG0711", "SO:0001217", "UniRef:UniRef50_B3PLV4", "UniRef:UniRef90_D1J7G2" ], "nt": "TTGATTATTACTTTATTAGTATATAAACCAATAAAGAAATTAATTAAAAATCGCCAAGAACTTATTCAAAAAAATATTGATACTTCAATATTAGCAAAAGAAAATGCTTTAAAAGTTCAAGAAGAAAATGATAAAAAAATTATTGAAGCTAATAATCAAGCAGCTTTAATAATTAATCATGCTAAATCAGAAAGTGAAAGAATTGTCAATGCAAGCACGATTGCTGCTAAAAAACAATCTGAACTTTTATTAGATCAAGCACAAATTTTGATTAATAAAAAGCAATCTGAGCTAGAACAAGCACAAAAGAAAATAATAGTAGAAAATGCTGTTGAATTAGCCAAGAAAATTATTGGCCGTGAAATTAGAAATGAAGATAACATTAAATTAATTAATGAAGTCTTAGAAGATTAA", "aa": "MIITLLVYKPIKKLIKNRQELIQKNIDTSILAKENALKVQEENDKKIIEANNQAALIINHAKSESERIVNASTIAAKKQSELLLDQAQILINKKQSELEQAQKKIIVENAVELAKKIIGREIRNEDNIKLINEVLED", "aa_hexdigest": "be3df468688ee76264db8a0e355bc9c6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "atpF" ], "truncated": "5-prime", "pseudogene": { "start": 22408, "stop": 22989, "observations": { "mutation": [ 22531 ], "stop": [ 22531 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7G2", "query_cov": 1.0, "subject_cov": 0.7098445595854922, "identity": 0.993, "score": 228.0, "evalue": 2.8e-74, "gene_start": 1, "gene_end": 137, "reference_start": 57, "reference_end": 193, "reference_sequence": "MFQNFANLLSQSQNAIAAAKPSASAKIAEELNKTFNGLTFNWPFFVFSLISLGLVVLIITLLVYKPIKKLIKNRQELIQKNIDTSILAKENALKVQEENDKKIIEANNQAALIINHAKSESERIVNASTIAAKKQSELLLDQAQILINKKQSELEQAQKKIIVENAVELAKKIIGREIRNEDNIKLINEVLED" }, "paralog": false, "identity": 0.9948186528497409, "score": 344.0, "evalue": 1.94e-122, "description": "Internal stop codon at 22531. Nonsense mutation around 22531.", "psc": { "uniref90_id": "UniRef90_D1J7G2", "gene": "AtpF", "product": "ATP synthase subunit b", "uniref50_id": "UniRef50_B3PLV4", "cog_id": "COG0711", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7G2", "UniRef:UniRef50_B3PLV4", "COG:COG0711", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLV4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLV4" ], "product": "ATP synthase subunit b" } }, "id": "DFOKDJOJIG_85", "locus": "GCA013367855_00045" }, { "type": "cds", "sequence": "contig_1", "start": 23063, "stop": 23530, "strand": "+", "frame": 2, "gene": "atpH", "product": "F0F1 ATP synthase subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "SO:0001217", "UniRef:UniRef50_D1J7G3", "UniRef:UniRef90_D1J7G3" ], "nt": "TTGGCATTAGTTTCTAATGAAGCTGCTGAAATTACAAGACTTTTAAAGAAAAATAAAGATTATTTGTATGTTTTAAATTCTTATGATATTGACGAAAATGAAAAATATCGTCTAATTGATGAAGCTTTTTCAAGATACAATTTTTATTTAGTAAATACTCTTAAATTAGCAGCTAAGCAACATGTGATTAAATACATTATTGTAATATTAACAAAATTTGTTGAACTTTCAAATGAAAAAAGAAACATTAAATACGGGTTTGTTTATACAATATCGCCTTTATCTGAAAGAGAATTAAAAAGGTTAGAAGTAAAACTTTCAAACGAGTTAAAGGCTAATATTCATTTAACAAATGAAATTGATCCCAAATTAATAGCGGGAATCAAAATAAAAGTTGACGATTATTTAATCGATAATTCAATAGTCAACAAATTAAATGACTTTAAAAAAGTCGTAGAAAATAATTAA", "aa": "MALVSNEAAEITRLLKKNKDYLYVLNSYDIDENEKYRLIDEAFSRYNFYLVNTLKLAAKQHVIKYIIVILTKFVELSNEKRNIKYGFVYTISPLSERELKRLEVKLSNELKANIHLTNEIDPKLIAGIKIKVDDYLIDNSIVNKLNDFKKVVENN", "aa_hexdigest": "c2aedb1828d349ce96d24aa5aed5c9c3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7G3", "query_cov": 1.0, "subject_cov": 0.8659217877094972, "identity": 0.987, "score": 293.0, "evalue": 4.58e-100, "valid": true, "gene": "AtpH", "product": "F0F1 ATP synthase subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_D1J7G3", "cog_id": "COG0712", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_D1J7G3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7G3" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "DFOKDJOJIG_86", "locus": "GCA013367855_00046" }, { "type": "cds", "sequence": "contig_1", "start": 23543, "stop": 24289, "strand": "+", "frame": 2, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGTCATTAGAACCAAATGATTTGTCAGCAATCATTAAGGCGCAAATAAAACACTTTAATGAAGACATATCTAAACAAGAAATTGGCCGTGTAATAACAGTTGGTGATGGTATTGCCTTAGTAAGTGGCTTGAGCAATGTAGAATATGGCGAATTGGTAAAATTCGAATCCGGCGTTATTGGAATGGCCTTGAATCTTGAAGAAGATTTAGTTGGTATAGTTGTCATGGGTGATGACCGTAACATTGTTGAAAATAGTACAGTTTATCGTACTAAACAAGTTATATCAACACCTGTTGGTAACCATTTATCTGGCCGTGTGTTAGATGCACTTGCTAACCCAATTGATGGAAAAGGTCCAATTCAATATGAATATACTAGACCAATTTTTAAGATTGCACCTGGTGTTATGACTAGAAAAGAAGTTAATCAACCACTTGAAACCGGGATTTTGGCAATTGATGCTATGATTCCTATTGGTAAAGGTCAACGTGAATTGATTATTGGCGATAGACAAACAGGCAAGACTGCTATAGCCATTGATGCAATTTTAAATCAAAAAGGTCGCAACGTTTATTGTATTTATGTTGCTGTAGGTCAAAAGAATTCAACTATTGCACAAATAGTTGATCAATTAGCAAAACATGATGCATTAAAATATACAACAATTATTTCTGCATCAGCATCAGACAGTGCTCCTTTACAATATATTGCACCATATACAGGAATTACTATTGCCGAAGAATGA", "aa": "MSLEPNDLSAIIKAQIKHFNEDISKQEIGRVITVGDGIALVSGLSNVEYGELVKFESGVIGMALNLEEDLVGIVVMGDDRNIVENSTVYRTKQVISTPVGNHLSGRVLDALANPIDGKGPIQYEYTRPIFKIAPGVMTRKEVNQPLETGILAIDAMIPIGKGQRELIIGDRQTGKTAIAIDAILNQKGRNVYCIYVAVGQKNSTIAQIVDQLAKHDALKYTTIISASASDSAPLQYIAPYTGITIAEE", "aa_hexdigest": "9c6688dc667927208fb85f72a3d4e291", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.9717741935483871, "subject_cov": 0.9450980392156862, "identity": 0.598, "score": 292.0, "evalue": 6.86e-97, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "DFOKDJOJIG_87", "locus": "GCA013367855_00047" }, { "type": "cds", "sequence": "contig_1", "start": 24290, "stop": 24991, "strand": "+", "frame": 2, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9F0" ], "nt": "ATGGCAAGAGGTAAGGATGTTTTAATTATATATGACGATTTGTCAAAGCATGCTGTTGCATATCGTACATTATCATTACTATTAAGAAGACCACCTGGACGTGAAGCTTATCCTGGAGACGTCTTTTATATTCACTCACAACTTCTAGAACGTGCAGCTAGAGTAAATTCTGATTATGGAGGCGGTTCAATTACTGCTTTGCCAATTATTGAAACTCAAGCTGAAGATATTGCAGCATATATTCCAACAAATGTTATTTCGATAACTGATGGTCAAATCTTTACTAAGGAAGCATTGTTTAACTCTGGCCAACGTCCTGCTATTGATATCGGTTATTCAGTTAGCCGTGTTGGATCAGCAGCACAAACAAAATTAACAAAAAAGGTTGTATCAAGTCTAAAATTGGAATTAGCACAATACAATGAAATGTTAGCATTTGCTCAATTTGGTAGTGATCTAGACCAAGCAACTAGAACTGTTTTGGATCACGGTGCTAAAGTGTATGAATTATTGAAACAACCTCAATATTCTCCATATTCTTCAATAGAACAAATAATTATTCTATTTTGCACAAAATATCGTTTAATAAATGTAATTCCAAAAGAACAAATATTAAAATATAAAACTGATATTTTAAATTACTTTAGAACTAATCCTAATGCTATTAATACATTAAGAGCCGTTTCTGAAGCAAATGATTGA", "aa": "MARGKDVLIIYDDLSKHAVAYRTLSLLLRRPPGREAYPGDVFYIHSQLLERAARVNSDYGGGSITALPIIETQAEDIAAYIPTNVISITDGQIFTKEALFNSGQRPAIDIGYSVSRVGSAAQTKLTKKVVSSLKLELAQYNEMLAFAQFGSDLDQATRTVLDHGAKVYELLKQPQYSPYSSIEQIIILFCTKYRLINVIPKEQILKYKTDILNYFRTNPNAINTLRAVSEAND", "aa_hexdigest": "b468ffcffd554434878d00167a9a7f5b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M9F0", "query_cov": 0.9141630901287554, "subject_cov": 0.841897233201581, "identity": 0.653, "score": 274.0, "evalue": 7.09e-90, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9F0" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9F0" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "DFOKDJOJIG_88", "locus": "GCA013367855_00048" }, { "type": "cds", "sequence": "contig_1", "start": 25105, "stop": 25323, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00231.24" ], "nt": "ATGGAAAGTTTGCAAAAAATAAAACATAGAATTAATTCGGTTAATTCAACTAAAAAAATTACCAAAGCAATGGAGCTAGTTGCAACTGCTAAGTTTGCTAAAATTAAAAATCAATTTGAAAATATCAATAATTATTATCAAAGTGTTGAAAGAGTTTTTTTAAACCTAATTAAAAATTCTGATCAAAATATTGATAAATTATTGAACAGCACTTCTTGA", "aa": "MESLQKIKHRINSVNSTKKITKAMELVATAKFAKIKNQFENINNYYQSVERVFLNLIKNSDQNIDKLLNSTS", "aa_hexdigest": "cc0a969792f7c436b658da87520ea468", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00231.24", "name": "ATP-synt", "length": 64, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 1.9285528981703304e-18, "score": 62.76412582397461, "start": 3, "stop": 66 } ], "seq_stats": { "molecular_weight": 8345.523499999996, "isoelectric_point": 9.85201015472412 }, "id": "DFOKDJOJIG_89", "locus": "GCA013367855_00049" }, { "type": "cds", "sequence": "contig_1", "start": 25399, "stop": 25986, "strand": "+", "frame": 1, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "KEGG:K02115", "SO:0001217", "UniRef:UniRef50_D1J7G5", "UniRef:UniRef90_D1J7G5" ], "nt": "ATGTATAAAAAAATTACTCAAACTGTTGGAAAAGAAGATATCCTAATAGTAGTTGGTAGCAAATTATTGAGTTTAATTCAAAAAGATAATAGCCATCATATTATTCAAATATTAACTCAAATTGGTGACAATCCAAGTTACGAAATCGCTCAAATATTGTCAAATAAGATTTATCATGTTTTAGAAATATCATGGCTAAAATCTATTAAACTTATTTATACTCAATACATTAATCCAATAAAATCCGAACCAATAGTAAAAGAAATTTTTCCAATTTCTATTGATACACTATCAAATAATGATAACAATTTAGCAGGGGAAGAAAGTTCAAAAAGTATTGATCCTTCTTGCTTTGAACCAAACCCTACTGTAATATTGCAAAATTCATTTTCAATATTCTTTGAAGCCTCTATATACAATGCCTTAGCAAATGCTAAGCTTGCAGAAATGAGCCAAAGAAGAACGGCAATGGAACAAGCAAGTGATAATGCACAAGATCTAATTAATGAATTAAATGTTCAATATAATAGTTCTCGTCAAGCAAAAATTACACAAGAAATTACTGAAATAATTAATGGATCAAATTAA", "aa": "MYKKITQTVGKEDILIVVGSKLLSLIQKDNSHHIIQILTQIGDNPSYEIAQILSNKIYHVLEISWLKSIKLIYTQYINPIKSEPIVKEIFPISIDTLSNNDNNLAGEESSKSIDPSCFEPNPTVILQNSFSIFFEASIYNALANAKLAEMSQRRTAMEQASDNAQDLINELNVQYNSSRQAKITQEITEIINGSN", "aa_hexdigest": "6ed4b614693c566f03fdcc8ec32644f5", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "5-prime", "pseudogene": { "start": 25105, "stop": 25986, "observations": { "mutation": [ 25321 ], "stop": [ 25321 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7G5", "query_cov": 1.005128205128205, "subject_cov": 0.6666666666666666, "identity": 0.995, "score": 362.0, "evalue": 6.82e-125, "gene_start": 1, "gene_end": 195, "reference_start": 99, "reference_end": 294, "reference_sequence": "MESLQKIKHRINSVNSTKKITKAMELVATAKFAKIKNQFENINNYYQSVERVFLNLIKNSDQNIDKLLNSASWKFKSKRNLYIVFGSDLGLCGPYNSEMYKKITQTVGKEDILIVVGSKLLSLIQKDNSHHIIQILTQIGDNPSYEIAQILSNKIYHVLEISWLKSIKLIYTQYINPIKSEPIVKEIFPISIDTLSTNNDNNLAGEESSKSIDPSCFEPNPTVILQNSFSIFFEASIYNALANAKLAEMSQRRTAMEQASDNAQDLINELNVQYNSSRQAKITQEITEIINGSN" }, "paralog": false, "identity": 0.9897959183673469, "score": 559.0, "evalue": 5.89e-205, "description": "Internal stop codon at 25321. Nonsense mutation around 25321.", "psc": { "uniref90_id": "UniRef90_D1J7G5", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_D1J7G5", "cog_id": "COG0224", "cog_category": "C", "kegg_orthology_id": "K02115", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7G5", "EC:7.1.2.2", "UniRef:UniRef50_D1J7G5", "COG:COG0224", "COG:C", "KEGG:K02115", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7G5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7G5" ], "product": "ATP synthase gamma chain" } }, "id": "DFOKDJOJIG_90", "locus": "GCA013367855_00050" }, { "type": "cds", "sequence": "contig_1", "start": 26003, "stop": 27490, "strand": "+", "frame": 2, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_020002442.1", "SO:0001217", "UniParc:UPI00036A1184", "UniRef:UniRef100_A0A2K9YSL9", "UniRef:UniRef50_Q5FKY0", "UniRef:UniRef90_D1J7G6" ], "nt": "ATGGCAAATAAAAAAACTAATGAAAATCAAGAAATTCAAGAAGAACAAAACCCAGTTGATGAATCTAAAAATTCAGAAAATTATGGCGTTATTAGCCAAATTTTGGGATCTGTTATTGACGTTAGATTTGAAGAAGGACATACTCCAAAAATCTTAAATGCCTTAAAAATTATTGATGAAGACAAAAAATTTAGCAATGAACAAATTCTTGAAGTTGCTCAACACGTAGGAAATGATACAGTTAGAACAATAGCAATGAACTTGACTATTGGTTTAAAGCGTGGTATGAAGGTTCTTGATTTGGGTTCTCCAATAAAGGCGCCTGTTGGTAAAGAAGTTCTATCTAGAATGTTTAATGTTCTTGGTGAACCTATAGACCAAAAAGGTGGTGTATTCAAACACAAAATGCCTATCCATAGAGCAGCGCCAAGCTACGAAGAACAAAAATCAACACTTGAAATATTTGAAACAGGAATTAAAGTTATTGACTTACTTGTTCCATATATTAAAGGTGGAAAGATAGGTTTATTCGGAGGAGCCGGAGTTGGTAAAACTGTTTTAATTCAAGAATTAATTAACAATATTGCAAAACAACACGGCGGTCTTTCTGTGTTTGCAGGTGTTGGAGAACGTACAAGAGAAGGTAATGACCTTTATCACGAAATGCAAGCAAGTGGTGTTCTAGAAAAAACTGCTCTAGTATTCGGTCAAATGAACGAACCTCCTGGAGCCAGAATGAGAGCACCATTTACTGCTTTAACTATCGCTGAATACTTTAGAGATGTTATGCATCAAGATGTGCTATTGTTTATTGATAACATATTCAGATTTACACAAGCAGGCTCTGAAGTTTCTGCATTGTTAGGCCGTATGCCTTCTGCTGTTGGATATCAACCAACATTGGCTGTTGAAATGGGTCAATTACAAGAAAGAATTACCTCAACCAATAACGGATCAATTACCTCTGTTCAAGCTGTTTATGTTCCTGCCGATGACTTAACAGACCCCGCACCAGCAACAACATTTACACACCTTGATGCAAAAACTGTTTTGGACCGTGATATTGCTGCTTTAGGTATTTACCCTGCTATCGATCCTTTGGCTTCATCATCAAGAATGGTCGATCCAAATATAATTGGCCTTGAACACTATAACACTGCTAGAGCAGTTCAAAATATTCTTCAAAGATTTAAGGAATTACAAGATATTATTGCTATATTAGGTATGCATGAATTAAGTGAAGAAGATAAGAAAGTTGTTGCAAGAGCAAGAAGAATAAGAAACTTCTTATCTCAACCTTTCTTTGTTGCCGAAAAATTCTCTGGTATTAAAGGACAATACATAAAATTGTCAGATACTATTAGATCATTTAAAGAAATTTTAAGTGGAAAATATGACGAATTGCCAGAAGAAGCATTCCTTTATGTGGGTACCATTGATGATGTTATAGAAAAAGCTAAAAGACTTGGGGTTAATGTTGAAAAATAA", "aa": "MANKKTNENQEIQEEQNPVDESKNSENYGVISQILGSVIDVRFEEGHTPKILNALKIIDEDKKFSNEQILEVAQHVGNDTVRTIAMNLTIGLKRGMKVLDLGSPIKAPVGKEVLSRMFNVLGEPIDQKGGVFKHKMPIHRAAPSYEEQKSTLEIFETGIKVIDLLVPYIKGGKIGLFGGAGVGKTVLIQELINNIAKQHGGLSVFAGVGERTREGNDLYHEMQASGVLEKTALVFGQMNEPPGARMRAPFTALTIAEYFRDVMHQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAVEMGQLQERITSTNNGSITSVQAVYVPADDLTDPAPATTFTHLDAKTVLDRDIAALGIYPAIDPLASSSRMVDPNIIGLEHYNTARAVQNILQRFKELQDIIAILGMHELSEEDKKVVARARRIRNFLSQPFFVAEKFSGIKGQYIKLSDTIRSFKEILSGKYDELPEEAFLYVGTIDDVIEKAKRLGVNVEK", "aa_hexdigest": "4a19b542e91cd0c2c735d0c3c4fad8a1", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00036A1184", "ncbi_nrp_id": "WP_020002442.1", "uniref100_id": "UniRef100_A0A2K9YSL9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036A1184", "RefSeq:WP_020002442.1", "UniRef:UniRef100_A0A2K9YSL9" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YSL9", "uniref90_id": "UniRef90_D1J7G6" }, "psc": { "uniref90_id": "UniRef90_D1J7G6", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q5FKY0", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q5FKY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5FKY0" ], "product": "ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "DFOKDJOJIG_91", "locus": "GCA013367855_00051" }, { "type": "cds", "sequence": "contig_1", "start": 27490, "stop": 27867, "strand": "+", "frame": 1, "gene": "atpC", "product": "ATP synthase epsilon chain", "db_xrefs": [ "COG:C", "COG:COG0355", "RefSeq:WP_175972169.1", "SO:0001217", "UniParc:UPI00158DBBEB", "UniRef:UniRef100_UPI00158DBBEB", "UniRef:UniRef50_A0A0C6FPC1", "UniRef:UniRef90_D1J7G7" ], "nt": "ATGGAAACTCCTAAGATACATATTATTATTAGTACTCCATTAGGAATATACTTTGATAAAGAAGCAAGTATTTGTACATTTACTACTACTGAAGGGCAAATTGGAATTATGTCAGGCGCAACTGAGTTTATGGCAGCCTTGGTTCCTTCAAAAATAATGGTTAATTTTTTAAATTCACCTGATTCTAAAGTTTTTTATATTGATAAGGGAATTGTTCAATTTAAAAATAATGTCTTATCAATAATAGTTAATCAAATTGATACTAAAGAAATTGAATTGGATCATAAATTTAGCCCTGAAGATCAAAAGAAATATACAATTATTGAAGAAGTTACTTTGAAAAAGAGCATTTTGAAAAGTCAAACAAACAATAAATAA", "aa": "METPKIHIIISTPLGIYFDKEASICTFTTTEGQIGIMSGATEFMAALVPSKIMVNFLNSPDSKVFYIDKGIVQFKNNVLSIIVNQIDTKEIELDHKFSPEDQKKYTIIEEVTLKKSILKSQTNNK", "aa_hexdigest": "19da97fd3b20d3b70400f8550fa7bfd8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158DBBEB", "ncbi_nrp_id": "WP_175972169.1", "uniref100_id": "UniRef100_UPI00158DBBEB", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158DBBEB", "RefSeq:WP_175972169.1", "UniRef:UniRef100_UPI00158DBBEB" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158DBBEB", "uniref90_id": "UniRef90_D1J7G7" }, "psc": { "uniref90_id": "UniRef90_D1J7G7", "gene": "AtpC", "product": "ATP synthase epsilon chain", "uniref50_id": "UniRef50_A0A0C6FPC1", "cog_id": "COG0355", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_A0A0C6FPC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0C6FPC1" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "DFOKDJOJIG_92", "locus": "GCA013367855_00052" }, { "type": "cds", "sequence": "contig_1", "start": 28124, "stop": 28771, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0", "UniRef:UniRef90_UPI00215D6119" ], "nt": "ATGAAAAAAACAGCAAAAGCTTTATTAATAATATGCACAACTATAATACCTAGTTTTGCTATGCCATTAATATCGGCGGGATGTAATAAAACAACAAACCATGAAACAAACAAAGAAAATACAATTATAGATAAACAGGCCCCTATTAAAAAACAAGAAAATGATTCTAATAACCCCAATAAATTAGAATCCAACAGTGAATTAGCAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAAATTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAATAACCTTGATAAAAAATTTAAAATAGAAATTTTAAAATTAGAAGCAGACAAGAAGAATAATTTAATATCGATCTCGTATAATATTTCGTTTAATGATGGAAATAAAATCACTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAATAACGAAAGCTATTTAAATGGATTATTGAATGATAATGACACAATATTTAATTTAACAACTAATTTCAAAAAAGGTAATGAAATAGATGCAAGCCAACTGGATGCATATCATTTAGCTAATGGCCAAGGTTCTATTAGTTTATTGGCATCTAATAAAAATGTTTATGTTCCCAAAGATCTAATCTTAGAATATACTTGA", "aa": "MKKTAKALLIICTTIIPSFAMPLISAGCNKTTNHETNKENTIIDKQAPIKKQENDSNNPNKLESNSELAEAVKKANFQYKDIDKISMFDKLNKENLSNNLDKKFKIEILKLEADKKNNLISISYNISFNDGNKITKLENQKYSLTGLSTNNESYLNGLLNDNDTIFNLTTNFKKGNEIDASQLDAYHLANGQGSISLLASNKNVYVPKDLILEYT", "aa_hexdigest": "752d02474c45e9fa6e83df7e45013ae8", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 28124, "stop": 29074, "observations": { "mutation": [ 28769 ], "stop": [ 28769 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D6119", "query_cov": 1.0, "subject_cov": 0.41505791505791506, "identity": 0.9490000000000001, "score": 377.0, "evalue": 5.84e-127, "gene_start": 1, "gene_end": 215, "reference_start": 1, "reference_end": 210, "reference_sequence": "MKKTAKALLIICTTITPSFAMPLISAGCNKTTNHETNKENTIIDKQAPIKKQENDSNNPNKLESNSELAEAVKKANFQYKDIDKISMFDKLNKENLSNNLDKNFKIEILKLEADKKNNLISISYNISFNDGNKITKLENQKYSMTGLSTNNESYLNGLLNDNDTMFNLTTNLKKGSEIDAYHLANGQGSISLLASNKNVYVPKDLILEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSIDSFNEGDIEQTLRNNFIPKFENSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVAGFKGIEKVDDEQAKKIVDKLTFGDVYFPEKVKTFSQSRLNKLNLYIKDENGKAQLENPLNFVEYFTKNGINNFNKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKN" }, "paralog": false, "identity": 0.9462025316455697, "score": 585.0, "evalue": 2.71e-211, "description": "Internal stop codon at 28769. Nonsense mutation around 28769.", "psc": { "uniref90_id": "UniRef90_UPI00215D6119", "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D6119", "UniRef:UniRef50_UPI00215CD1C0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 24115.03900000002, "isoelectric_point": 8.318049430847168 }, "id": "DFOKDJOJIG_93", "locus": "GCA013367855_00053" }, { "type": "cds", "sequence": "contig_1", "start": 29192, "stop": 30202, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0", "UniRef:UniRef90_UPI00215CD1C0" ], "nt": "GTGGGATTTAAAGGCGTTGAAAAAGTAAGCGATGAACAAGTTAAAAAAATAGTAGATAAATTGACCTTTGGTGATGTTTATTTCCCTGAAAAAGTTAAAACCTTTAATCAAAGCCGTTTAAACAAATTAAATTTATATATTAAAGATGAAAACGGAAAAGCTCAACTAGAAAATTCTATAGATTTTGCAGAATATTTTACAAAAAATGGAATTAACAATTTTAATAAAAATTATATAGTTAAATATGATAAAGCCGAAAAGAAATTAATTACAACATTAAAATTGAATGAAAAAGAATACACAATTGAAACAAAATTAACGCATGAATCAAAACCAGATGAATGGGTCAACGAAGTAGGTAAGGAATTTTTAGGAAATCCAAATGAAATTTTGGCAAGTGATTTCTCTGCTACAAATTTAAAATTGAATAAAAATGTTGGCGATAGCCTTGATAGTTTAAATTTTATTAAATTGTCTTCAATAAAAAGACCATCAAAAAATTCAGAGGAATTAAAACTCAACGAAGAAGGTAAGGCAACGTTAATTTATGAATTTAGAAATATATATGATAATTCAATATATACAAAAGAAGTCGTATTTGAAAATCTAAAAAAATTAGAAAAAGCTGATTATAAAAAACAACACGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTAAAAGCGGTTGGGCTACTGACGAAGAAATAAAGAGATTGGAAACTTTTTTGCCAGGCGAAAAAAAATCTAAAAAAAATAAGAGAATAAACATTGAACATTATATAAATAATGGCAATACAGAAATACTTGTTGGAAGAGTAATTGCTAAAAAAAATAAAGTTACTTTCGGCGATACATTGAACAGACAAAAGGAATTTGAAGAAATAATATTTGATGAAGAATTTATTGAAGCAGTTAAGAATTCGTCAGATAATAAAAGCGAAAATAAAATATTATCTAATGTAATAACAGAAGAAAACTCTAAACATAAAAATCAAAAGAATTGA", "aa": "MGFKGVEKVSDEQVKKIVDKLTFGDVYFPEKVKTFNQSRLNKLNLYIKDENGKAQLENSIDFAEYFTKNGINNFNKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKNSEELKLNEEGKATLIYEFRNIYDNSIYTKEVVFENLKKLEKADYKKQHASLIQKINDKKIIKSGWATDEEIKRLETFLPGEKKSKKNKRINIEHYINNGNTEILVGRVIAKKNKVTFGDTLNRQKEFEEIIFDEEFIEAVKNSSDNKSENKILSNVITEENSKHKNQKN", "aa_hexdigest": "d51c23d76c7a25dbcffae8e12dbe2720", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 28892, "stop": 30298, "observations": { "mutation": [ 30200, 30230 ], "stop": [ 30200, 30230 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215CD1C0", "query_cov": 0.9970238095238095, "subject_cov": 0.6203703703703703, "identity": 0.925, "score": 591.0, "evalue": 6.78e-209, "gene_start": 2, "gene_end": 336, "reference_start": 174, "reference_end": 508, "reference_sequence": "AYHLANGQGSISLLASNKNVYVPKDLILEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSIDSFNEGDIEQTLRNNFIPKFENSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVAGFKGIEKVDDEQAKKIVDKLTFGDVYFPEKVKTFSQSRLNKLNLYIKDENGKAQLENPLDFAEYFTKNGINNFNKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKNSEELKLNEEGKATLIYEFRNIYDNSIYIKEVVFENLKKVEKADYKKQHASLIQKINDKKIIKSGWATAKEIKKLETFLPSQKKSKKNKGINIEHYINNGNTEILVGRVITKKNKITFGDAFKRQKEFKEIVFDEEFVEAVKKSSNNKSENKILSNVITDENSKHKNQKNWIRLTKHEGKWCILFTFIEDTKSTPYYVQIEK" }, "paralog": false, "identity": 0.9273504273504274, "score": 865.0, "evalue": 2.55e-319, "description": "Internal stop codon at 30200, 30230. Nonsense mutation around 30200, 30230.", "psc": { "uniref90_id": "UniRef90_UPI00215CD1C0", "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215CD1C0", "UniRef:UniRef50_UPI00215CD1C0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 38999.708900000005, "isoelectric_point": 8.92192478179932 }, "id": "DFOKDJOJIG_94", "locus": "GCA013367855_00054" }, { "type": "cds", "sequence": "contig_1", "start": 30537, "stop": 31178, "strand": "+", "frame": 3, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5ADF", "UniRef:UniRef90_UPI00215D5ADF" ], "nt": "ATGAAGAAATCGAAAAAAATTTTGTTGACCATGGGAGGGATACTTCCCTTGGTTATTTCAGCGCCATTAGTTGCTGCTGGATGCAAGGAGCCAAATAAACCAGAAGTTAAACCAGAGGATCCAAAGAAACCAGAAGGTGAAAAACCCGGAACAACTCCCGAAGTTAAGCCAGAATCTAACAGTGATTTAAATGATTTAACAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAGGTTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAACAACTTCGATAAAAAATTCAAAGTAGAAGTTTTGAAATTAGAAGTAGATAAGAAAATTAATTCAGTATTAATTACATACAACATTTCGTTCAATGACGGAAGTAAAGACGCTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAACAATGAAAGCTATTTAAATGGATTATTAAACGATAAGGATACGGTATTCAATTTAGGTGATGCTTCTAATGAAGATAGTTTAACAACAGTTGAACAGTTAGATGCATATTACTTAGTTGATGGTCGAGGTTCTATTAGTTTATTAGCATCTAATAAAAATGTTTATGTTCCTGAAGGTCTAATGCTGGAATATACTTGA", "aa": "MKKSKKILLTMGGILPLVISAPLVAAGCKEPNKPEVKPEDPKKPEGEKPGTTPEVKPESNSDLNDLTEAVKKANFQYKDIDKVSMFDKLNKENLSNNFDKKFKVEVLKLEVDKKINSVLITYNISFNDGSKDAKLENQKYSLTGLSTNNESYLNGLLNDKDTVFNLGDASNEDSLTTVEQLDAYYLVDGRGSISLLASNKNVYVPEGLMLEYT", "aa_hexdigest": "a05d07000229da3d9026373406c8f55b", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 30537, "stop": 31481, "observations": { "mutation": [ 31176 ], "stop": [ 31176 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D5ADF", "query_cov": 1.0892018779342723, "subject_cov": 0.5686274509803921, "identity": 0.875, "score": 365.0, "evalue": 5.7e-124, "gene_start": 1, "gene_end": 213, "reference_start": 1, "reference_end": 232, "reference_sequence": "MKKSKKILLTMGGILPLVISAPLVAAGCKEPNKPEVKPEDPKKPEGEKPGTTPEVKPEDPKKPEGEKPGTTPEVKPKSNSDLNDLTEAVKKANFQYKDIDKISMFDKLNKENLSNNFDKKFKVEVLNLEVDKKINSVLITYNISFNDGSKDAKLENQKYSLTGLSTNNESYLNGLLNDKDTVFNLGDASNDDSLTTVEQLDAYYLVDGLGSISLLASNKNVYVPEGLMLEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSINSFKEGDIEQTLKNNFIAKFEKSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVSGFKGVEKVDDEQVKKIVDKLTFGDVYFPEKIKDF" }, "paralog": false, "identity": 0.9099099099099099, "score": 584.0, "evalue": 1.41e-212, "description": "Internal stop codon at 31176. Nonsense mutation around 31176.", "psc": { "uniref90_id": "UniRef90_UPI00215D5ADF", "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI00215D5ADF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D5ADF", "UniRef:UniRef50_UPI00215D5ADF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D5ADF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5ADF" ], "product": "variable surface lipoprotein" } }, "id": "DFOKDJOJIG_95", "locus": "GCA013367855_00055" }, { "type": "cds", "sequence": "contig_1", "start": 31560, "stop": 32318, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATAAGAAACTTGCCAATGCAAATATTGAAGTTTCAGGATTTAAAGGCGTTGAAAAAGTAAGTGATGAACAAGTTAAAAAAATAGTAGATAAATTGACCTTTGGTGATGTTTATTTCCCTGAAAAAATAAAAGAAATTTCAAAAGACGAAACATTTTTGAATGAGTTGCCTTTATATATCAAAGATGAACATCAAAAAATTAATCCCGAAAAATTTGTTGATTCTGAATATTTAATTAAAAATGGGATCACCGAATTTGATAAAAATGAATCTGGGCAACTTAACTACAAAGTCAAATATGATGAAAATGCAAAAAAATTGTCTGTAACATTAAAATTAAACGGCAAGGAATATAATGTTGAGACAACATTAACTCATGAATTAAAATTAGATGATTGGGTAAATGAAGTTGGAAAAGAAATTAATGGCGATTCAAATACAATTCCTGTAAATGAATTTTCTAATAAAAATTTAAAATTGAATAAAAAAAATAATGACTTTATCAATGGTTTAGATTTTATTAAATTAAATTCAATAAAAAGAGCAAAATCTTCTGAAGCAGAAAAAAAATTAAATTCAGAAGGTAAGGCAACATTAATTTATGAATTTAGAAATATATATGATAATTCAATATATACAAAAGAAGTCATATTTGAAAATCTAAAAAAAGCAGAAAAAGTTGATTACAAAAAACAACATGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTACTAATAATAAATGA", "aa": "MKNKKLANANIEVSGFKGVEKVSDEQVKKIVDKLTFGDVYFPEKIKEISKDETFLNELPLYIKDEHQKINPEKFVDSEYLIKNGITEFDKNESGQLNYKVKYDENAKKLSVTLKLNGKEYNVETTLTHELKLDDWVNEVGKEINGDSNTIPVNEFSNKNLKLNKKNNDFINGLDFIKLNSIKRAKSSEAEKKLNSEGKATLIYEFRNIYDNSIYTKEVIFENLKKAEKVDYKKQHASLIQKINDKKIITNNK", "aa_hexdigest": "50a74b6bfc727145fe4413a964ba7b84", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29195.825600000037, "isoelectric_point": 8.772873878479004 }, "id": "DFOKDJOJIG_96", "locus": "GCA013367855_00056" }, { "type": "cds", "sequence": "contig_1", "start": 32322, "stop": 32588, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H0", "UniRef:UniRef90_UPI00093DEE59" ], "nt": "TTGCAAGAAGAAATAAACAAAATAAGTGCCTTTAAAAGCAATAAAGAAACCTATTCTTATAAAGATAATGAAGGTAAAGAACAAAGTGAAACATTATCTGGTAGATTTCAAATTAAAGGAAAATCAGGCAATTTAGAATTGCATTTTGGGGATAATGGTAAAGCTCGCGAATCATTTAAAGAGTTAAAATTTGACAATGATTTTCTTGAAGCAATAAAAAGTAAAACATTAGTTAATGTTGTTAAGGATTCTAAACACGACCAATGA", "aa": "MQEEINKISAFKSNKETYSYKDNEGKEQSETLSGRFQIKGKSGNLELHFGDNGKARESFKELKFDNDFLEAIKSKTLVNVVKDSKHDQ", "aa_hexdigest": "1c2f37c15484ee6cb0ce7b545aa24dc2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 32315, "stop": 32684, "observations": { "insertion": [ 32339 ], "stop": [ 32586 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00093DEE59", "query_cov": 0.9318181818181818, "subject_cov": 0.6721311475409836, "identity": 0.89, "score": 140.0, "evalue": 2.9e-41, "gene_start": 7, "gene_end": 88, "reference_start": 9, "reference_end": 90, "reference_sequence": "MSFTRRDKKIGAFKNNKETHSYKDNEGKEQSETLSGRFKIKGKAGNLELHFGDNGKASESFKELKFDNDFLEAIKSKTLVNVVKGSKNNQWVRFTQHNGKWCIVFAFKDDPELTPYYVQIEQ" }, "paralog": false, "identity": 0.9016393442622951, "score": 221.0, "evalue": 5.75e-76, "description": "Internal stop codon at 32586. Frameshift due to insertion around 32339.", "psc": { "uniref90_id": "UniRef90_UPI00093DEE59", "uniref50_id": "UniRef50_D1J7H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00093DEE59", "UniRef:UniRef50_D1J7H0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10104.07999999999, "isoelectric_point": 6.737318992614747 }, "id": "DFOKDJOJIG_97", "locus": "GCA013367855_00057" }, { "type": "cds", "sequence": "contig_1", "start": 32796, "stop": 33749, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H1", "UniRef:UniRef50_UPI00215D9390", "UniRef:UniRef90_D1J7H1" ], "nt": "ATGACTAAGAAAAAAGTATTATTTAAAATATTTGCTATGGGTGCTATTTTAGCCGGAGGCGTTTTTAGTTTAAATTCATGTATTTTTAAATCAAATGATACAGAAAATAAAGATAATAGTTTTGAAAATAACACTAATAACGGTCAAAGGAACCAACATGATTTAAGCACCGAAGATAATAATTCAAGAGATTTGATAGTTCCTGAATCAAATAATAAGCCCGAAAATGATTCTATCCCTCAACCTGAATCAAAACCAAATATTATCCCAACGCCTGAAATAACGCCCAAACCAGCGCCTGAAATAACGCCCGAACCAACGCCCAAACCAGCGCCTGAAATAACGCCCAAGCCAAATGAAAATGACTATAATAAAGCCAATCATAATCAAACTCATTTTGTAAGGCCACCTTTTATTGCTAAATTTAAAATTAAAAATACTGATTTTTCAATAGCGACAATTTTTAACCATTTAGATAGTCCTGGTACTAGAAAAAAAGGAGAATACATTGAACCAAAGCCATCGATTTTATTGGCTGATAGTTCAAATAGACAGTCAGATTTGAATAAACAAGGAGCCCAAGAAGTAAGTGAATTTCTAGGCTTAATAGATGTTATTAATGAAAACAAAGATTTGGCCAATTTTGTTATTTACGGTGGCGATACTAATATAAAAAATGAAAACTTTTATTTAGCAAGAAAATATCCCGAAAATATTCAATTTACTTTAGATAGAGACTTAAAATTAAAAAAATATGACCAAAAATTTCTAACATCGTTAGGAACAAGTGGAAAATATTCAGAACAATACGACAAAATGTTCTTTATTAATCAATTGCCCAATACATTTAAACCAAAAATAATTACCAATGGTCTTTTACCTTATAAGGTTGATATTTATAGAACGTTTAATGATGTTATTTCAAAAGCAGAACTTGAAAACTTAAAAGGATGA", "aa": "MTKKKVLFKIFAMGAILAGGVFSLNSCIFKSNDTENKDNSFENNTNNGQRNQHDLSTEDNNSRDLIVPESNNKPENDSIPQPESKPNIIPTPEITPKPAPEITPEPTPKPAPEITPKPNENDYNKANHNQTHFVRPPFIAKFKIKNTDFSIATIFNHLDSPGTRKKGEYIEPKPSILLADSSNRQSDLNKQGAQEVSEFLGLIDVINENKDLANFVIYGGDTNIKNENFYLARKYPENIQFTLDRDLKLKKYDQKFLTSLGTSGKYSEQYDKMFFINQLPNTFKPKIITNGLLPYKVDIYRTFNDVISKAELENLKG", "aa_hexdigest": "f120ea2f0b889c43911227d9f3472337", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00215D9390", "query_cov": 0.8832807570977917, "subject_cov": 1.0256410256410255, "identity": 0.818, "score": 382.0, "evalue": 6.79e-131, "valid": false, "uniref50_id": "UniRef50_UPI00215D9390" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9390", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9390" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 32796, "stop": 34052, "observations": { "mutation": [ 33747, 33918 ], "stop": [ 33747, 33918 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7H1", "query_cov": 1.0378548895899053, "subject_cov": 0.6553784860557769, "identity": 0.96, "score": 511.0, "evalue": 2.42e-178, "gene_start": 1, "gene_end": 317, "reference_start": 1, "reference_end": 329, "reference_sequence": "MTKKKVLFKIFAMGAILAGGVFSLNSCIFKSNDTENKDNSFENNTNNGQRNQHDLSTEDNNSRDLIVPESNNKPENDSIPQPESKPNIIPTPEITPKPAPEITPEPTPKPAPEITPEPTPKPAPEITPKPNENDYNKANHNQTHFVRPPFIAKFKIKNTDFSIATIFNHLDSPGTRKKGEYIEPKPSILLADSSNRQSDLNKQGAQEVSEFLGLIDVINENKDLANFVIYGGDTNIKNENFYLARKYPENIQFTLDRDLKLKKYDQKFLTSLGTSGKYSEQYDKMFFINQLPNTFKPKIITNGLLPYKVDIYRTFNDVISKAELENLKGWKSKNNDKSTIRSQISDHAPVFTDVVFSNPIETLKVNESLRKNQTLSKEQNTIRIAHWNILNFGGPNQDAKTMAIATTIYKSGFDIIGLTEINDGRGNYVQNIVDLLNEYAGKERYKFVLQNKKDTNIRPEYLQSNKFGKAQQEQVAVIYDSQNFEFVSSRSYLKPIKYYKDN" }, "paralog": false, "identity": 0.9651162790697675, "score": 712.0, "evalue": 3.79e-260, "description": "Internal stop codon at 33747, 33918. Nonsense mutation around 33747, 33918.", "psc": { "uniref90_id": "UniRef90_D1J7H1", "uniref50_id": "UniRef50_D1J7H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7H1", "UniRef:UniRef50_D1J7H1" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35949.0174, "isoelectric_point": 6.926933860778808 }, "id": "DFOKDJOJIG_98", "locus": "GCA013367855_00058" }, { "type": "cds", "sequence": "contig_1", "start": 33963, "stop": 34268, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B017", "UniRef:UniRef90_UPI00215DB2CF" ], "nt": "ATGGCTATTGCTACTACAATTTATAAGTCTGGTTTTGATATAATAGGCTTAACTGAAATTAATGATGGAAGAGGAAACTATGTGCAAAATATAGTTAACCTTTTAAATGAATATGCAGGAAAAGATAGATATAAATTCGTATTACAAAATAAAAAGGATACAAATATAAGGCCTGAATATTTACAATCAAATAAGTTTGGAAAAGCGCAACAAGAACAAGTTGCAGTGATTTATGATAGTCAAAATTTTGAATTTGTTTCAAGCCGAAGCTATTTAAAACCAATTAAATACTACAAAGATAACTAA", "aa": "MAIATTIYKSGFDIIGLTEINDGRGNYVQNIVNLLNEYAGKDRYKFVLQNKKDTNIRPEYLQSNKFGKAQQEQVAVIYDSQNFEFVSSRSYLKPIKYYKDN", "aa_hexdigest": "d6c8f3972447ba2c88f7a52bdd490feb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00215DB2CF", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.96, "score": 192.0, "evalue": 3.33e-62, "valid": true, "uniref50_id": "UniRef50_A0A449B017" }, "pscc": { "uniref50_id": "UniRef50_A0A449B017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B017" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11745.086799999992, "isoelectric_point": 9.07149143218994 }, "id": "DFOKDJOJIG_99", "locus": "GCA013367855_00059" }, { "type": "cds", "sequence": "contig_1", "start": 34861, "stop": 35286, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATGCAATTGAAAAAATTAAATCAGCATTTAGCTTTAATGATGTTTTAGTATTATCTGAATTAGTAGGTGAAGATAAACCAATCAATGAAACTGCTTTGGTTAAATTAATCAATTTAGTATTTGGAAAAAGTAATTTTAATGACTCTGTAATCTATAATGCATTAAGAAATCTAAACAATAATTCAGATGAATCAAAACGTTCAAACATGAATACCTTAAATAGTTCTGTTTCAAATGCGTTAGGAAGTATTTTCAAATCTTCAAAAGCCTCTGATAAAGATGACCCTGATAATATTGAATATTCCGCAGATGTTTTAAAATTGCTTGATACAATTTATAAATTGCTTGCAAAGGCATACAATAATGATAAGGATGCAACTTCACCAACATATAAAGTTAGGGCACAATCTGAAGCATGA", "aa": "MNDAIEKIKSAFSFNDVLVLSELVGEDKPINETALVKLINLVFGKSNFNDSVIYNALRNLNNNSDESKRSNMNTLNSSVSNALGSIFKSSKASDKDDPDNIEYSADVLKLLDTIYKLLAKAYNNDKDATSPTYKVRAQSEA", "aa_hexdigest": "c659de675599e90d68543848f042ab84", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15560.170799999978, "isoelectric_point": 4.977924919128417 }, "id": "DFOKDJOJIG_100", "locus": "GCA013367855_00060" }, { "type": "cds", "sequence": "contig_1", "start": 35731, "stop": 36420, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGCAAATAAAGATAAATTTAAAGCATTTATTAAAAATGCAGTAAATGAAATAACTTCTAACAAAAACTTAATTAACAAATCTATTGAATATGCATTGAATTTATTCAACAAGAAATATTCAATTAGTTCAAGTAGCGCTCAAATTCAAGGAATTGCAAATCTTTTGACTAGATTAATTAACAAACTAGGTTCAATGAACGTATTTAGTAATATATTGGGAGATTTCATTGATTTGTTTGTTAATGTTGATTTCCTTGACAATAATGGTCATTTCAATGGAAATGCTTTGGCATCCAAACTTCTTGAATCTATTAAAAAGATTGAATACCATAACTATTTAACAACAGAAAATTTAAGTGAATTATTTGAAACAATATTAGATAAAAATATTGATAAAAATGTCTTGTATAATGAATTGAATTTCGCTTATTCATATATAGTTGACAATCTTGATAAATTTAAACAAGCATTTTCTGCTAAAAGCAATTCTACAACATTCAACGAAGTAGATAAAACTAAACAAGATAAGGAACACGAACAATTCCTAAAGAATCTAGAAAAATTAATTTACAATGCCTTAAAAGCTCTAAATGGATCAATAAAAGCAGATAATAAAAATGGAAAAGAAGCATTAGTTGATTTTGTATATAATGTTTTGAAGGATCAAATTAAGAAATTTAATTGA", "aa": "MSANKDKFKAFIKNAVNEITSNKNLINKSIEYALNLFNKKYSISSSSAQIQGIANLLTRLINKLGSMNVFSNILGDFIDLFVNVDFLDNNGHFNGNALASKLLESIKKIEYHNYLTTENLSELFETILDKNIDKNVLYNELNFAYSYIVDNLDKFKQAFSAKSNSTTFNEVDKTKQDKEHEQFLKNLEKLIYNALKALNGSIKADNKNGKEALVDFVYNVLKDQIKKFN", "aa_hexdigest": "9f5702bfe1a7e631863525e4d02a00b2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26137.36600000003, "isoelectric_point": 8.749987602233887 }, "id": "DFOKDJOJIG_101", "locus": "GCA013367855_00061" }, { "type": "cds", "sequence": "contig_1", "start": 36460, "stop": 36750, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAATGTGTATTTTCAATATTAGACAATAAGGCAAAGAAAGAAATTAAAGATGAATTAAAAAATTCAGTTGATATTTTCTTGCAAAGTAATAAATTTAAACAATATATCAAACACAATTTATTAAAAATAAAAGAAAAAATTCTAAAGGTTGATTCAAAAGCTTCTGAATTAGGCAATGAAATAGTTGAAACATTAATGAAGGTAATTTTTGATTCAAATAATACAAAAGAATTTATACTAACATTATTAAATTCATTTATAGATTTAGGCGATAACAATCAATGA", "aa": "MFKCVFSILDNKAKKEIKDELKNSVDIFLQSNKFKQYIKHNLLKIKEKILKVDSKASELGNEIVETLMKVIFDSNNTKEFILTLLNSFIDLGDNNQ", "aa_hexdigest": "1d82331595f1c5ffffe2754c5a1c95b9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11153.892899999988, "isoelectric_point": 8.644839668273928 }, "id": "DFOKDJOJIG_102", "locus": "GCA013367855_00062" }, { "type": "cds", "sequence": "contig_1", "start": 37330, "stop": 37524, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATACATTGATGAAATAAATATTTCAAGCTTTAAGGGTGATGTACAAGAAAACCTTCAACAAATAATTTCATATATAAAATCATTTGTTGAAACAAGTTACAATGAAGATCTTTTGCCTTTAATAAAAGAACTAATTAAAAAGTTATTTGATGATAATAATTTAAAAGATTTAAATAATATTTCTCAATGA", "aa": "MKYIDEINISSFKGDVQENLQQIISYIKSFVETSYNEDLLPLIKELIKKLFDDNNLKDLNNISQ", "aa_hexdigest": "ca8f3ab292e5527901cf62fcaba3ec50", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7524.463199999997, "isoelectric_point": 4.504399299621583 }, "id": "DFOKDJOJIG_103", "locus": "GCA013367855_00063" }, { "type": "cds", "sequence": "contig_1", "start": 37747, "stop": 38040, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGAATTTATCAATAAAATATTGAAAAATCTTGCAGACAACAAAGGCTTTGATGCGTTAGAACAATTGCTAGAAAATGTTTTATCAAATAAAGATATAACCCAAAAATTAGTCGAATTTTTAGTTGCGTTTTTAGAAATTGAAGCAAAAACAAAATTGAATTCTGATGAAATTAACCAATTAACTAATTATTTCAACAAGATTTTACCTACTTCATTGAAATCTAATTTATATAAAAACTTAAAAATTAAATTAACAAATGAATTGAAGTCAATAAATAAATATTCTTGA", "aa": "MPEFINKILKNLADNKGFDALEQLLENVLSNKDITQKLVEFLVAFLEIEAKTKLNSDEINQLTNYFNKILPTSLKSNLYKNLKIKLTNELKSINKYS", "aa_hexdigest": "fee64ba08acf44f53f6561e0b279fe6c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11219.904699999983, "isoelectric_point": 8.803174018859863 }, "id": "DFOKDJOJIG_104", "locus": "GCA013367855_00064" }, { "type": "cds", "sequence": "contig_1", "start": 38326, "stop": 38520, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGATACATTAAATGAAGAAAATGTAAAAACTCTATTTTTGATATTAATCAAGAATTTAAATGAATTTGCAAATGCTGTTTTAGCAAACAACAACACAACAACACAAAAACAAAATGAAGGTTCAAAGCCAATTAAATTAGACAATGATTTAAATTTTGAGCAAATCTTCTTACTAATTAAAAAATTATGA", "aa": "MIDTLNEENVKTLFLILIKNLNEFANAVLANNNTTTQKQNEGSKPIKLDNDLNFEQIFLLIKKL", "aa_hexdigest": "fc79a82d4fa0cad09cbb7b3901b8aba1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7347.399199999999, "isoelectric_point": 5.179930686950683 }, "id": "DFOKDJOJIG_105", "locus": "GCA013367855_00065" }, { "type": "cds", "sequence": "contig_1", "start": 38971, "stop": 39369, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTAAACTTATTGAAAATTTTGATGAAAAAATTCAAATTTTAGATAATAAAAAAATTGACACATTTGATAAATTGAAAAACTATTTAAAATCAATATTTAGTGACTACTTCAACATTGAAAAAAATAAGGATTTAATTAATAATTTTGTTGATTTGCTACTCGAAAAAGATAATAAATCAGAAGGAATAAAATTTTCTGAATTAATAAAAACACTTTCTGTTCTTGCTTCTAAATCCGAACTTGTTAATTACGTTTTTAAAAAATTTGACATTAAATCTATTATATTAAATTACTTGGATTCATTTAAAGACAACAAACTATCTAACGACACTGAATTAAATTCACTATTACAAACCTTAATCACCAAAACTAGAAATTACATAGATAGTCAATGA", "aa": "MTKLIENFDEKIQILDNKKIDTFDKLKNYLKSIFSDYFNIEKNKDLINNFVDLLLEKDNKSEGIKFSELIKTLSVLASKSELVNYVFKKFDIKSIILNYLDSFKDNKLSNDTELNSLLQTLITKTRNYIDSQ", "aa_hexdigest": "bc68f4aa36c30052472cc21630a02af8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15531.654299999984, "isoelectric_point": 6.319438743591309 }, "id": "DFOKDJOJIG_106", "locus": "GCA013367855_00066" }, { "type": "cds", "sequence": "contig_1", "start": 39706, "stop": 41847, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATCTATTTGGTGATTTTTCTAATAAAAAATTACAAGAGCTAATTAAAAAAATAAAACAAGCAAACCTGATTACAATTTCTTTGGGATTTAACGACCTTTTTGAACAATCATCATTTTTTAAAGAAGCGTTTGATCTTTTAAGAGAAAACAAAAATAAAGAAGAAATTTTGAAATATTTTTCAAAAATTCTTCTTGAATTTAAAAAGAATTGTAAAAATTTTAAAGATTCATATTCAAAATTAATTTCAATCATTAAACAATATAATCCTAAGGCAAATATTAATCTCGTGGGTTATATATCGCCTTATTTGAAATTAACAAAATTAATTGACAAACAATACAATATTTCGTTGACTAATGATTGCATAGAAGAATTAAACAAATCATTAGCAAACATTGCCAAAACAAACAAGCTAAATTATTTAAACATTAATAATGACATTAAAAATCTCAAAGATAATGCCGATAAACTAAACAATAATTTCTTTGACTGCTTCCCATCTAATAATTTATACAAAAAATTAGCGCAAGATATTTTTATGAAAATGTCATTGCCTAATAATAAATATAAAGAATTAGTTGATAATAATTCTAATCTAAATGAATTTAGCCAATCACTTGAATTTAGCAAAACTCCTTCCGAAATTTTTTCAATGATTTTAGGAATAAATGGCAACAATGTAGATTCTTTTGAAAAAGAATATACATTCGAAGAAAATGCAGATATCAAAAATATTAATTCTTCATACAATTCAAACCATATAACCCTTCAATTAAAATCAATGTTAAATTCAAACAAAAACATTGAAGATAAAGAAATTTTGAATACATTTTCAATACTATTCGGATTAATAGGCTTGGATCCGAAAGATTTAAGCAACTTTAATAAATATTTATTAGAATTTCTAAAGAATAAAGAAAATAAAAAATCTATTATTAATTTTTTGAATCTTTTTATTGATTCAAAAAATTTAAGCAAATCGTTTCAAGATATCAACAATGCATTAAATAATGCTATTAATAAAAAATCATATGCAGGTTTAAACTTTGAAGAAATTAAAAAAATTGTTTATTCAACAACTACTTCTAAACAAAATATTTATGAAATTTTAAAAGAAATTTTCTATAACAAATCTTTAAGGCAAGATCTTAAAAGAAGCGAATATGCAAAATTATTTAAGGCTATATTTTCAGATGTATTCATTAAAAAAATATTAAAAAGGTTATTGCCTCAAACTACTGTTGATTTATTCAAAAATCTTTTCAATAACAAAGATATTGAAAAAGGTTTAGACAACATTTTTCAAAAAGTGTTGAATTTGTTAATTAATAATTCAGATAAATATTTTAAATTTGAAAAAATTGATGACTTTATTACTACATTGGTTCATGATTCAAGCAAAGATGTAAATGATTTAGTTAGCCTAATAATTAAAGAAATTTCAAAAGATAGCAAACTTGCAAACTACTTGACCGGAAAAATTGTTGATTCTTTATACAAATCATATGATTTAGATTTACTTGAAAAACCAAATATTTCTTTGTTTGTTTCAACATTTTTTGACAACATTTCAAACTTCGATGGTTTATCTCAAATATCAAACATTATTATTCAAATATTAATGAATTCTAAACTAGATGATGACCACCAAAATAGGGATTTAATTTCAGAAATTCTAAATGGAATTCTTTTTGATAAATACGACCTAAATAGGGATAACTCGCTTTTATTTAAAATCCTATCGTTTGAACCAAAAATACAAAATATTAACGACTACAAACAAGGATTAAAACACTTAGCTCTAAAATATGCAATTAAAAATAATGCATTAATGCCAAAAAACATAGGCAAATTAATTGATAAAGATTCAAGAGAAAGCATATTAAGATTTTATAAAAATATAGCATTAAATGCAAAGAAGAATTTAAACGAATTTGGTAGCGAAATATTTAAAGATATTATCTCGCTTCTAGTAAATGAATTATTTAAGAATAATAGTTTGTTCAGTCAATTGATTGATCAAATTGGAAATTATGTTGTTATTCAGCCTATCGTTGCTTTCATTAAATCAATTAATTACAAAAAGATATTCAACAATGAAGAAGAAATTCAAAAATTTGTTGAAAATGTTTGA", "aa": "MNNLFGDFSNKKLQELIKKIKQANLITISLGFNDLFEQSSFFKEAFDLLRENKNKEEILKYFSKILLEFKKNCKNFKDSYSKLISIIKQYNPKANINLVGYISPYLKLTKLIDKQYNISLTNDCIEELNKSLANIAKTNKLNYLNINNDIKNLKDNADKLNNNFFDCFPSNNLYKKLAQDIFMKMSLPNNKYKELVDNNSNLNEFSQSLEFSKTPSEIFSMILGINGNNVDSFEKEYTFEENADIKNINSSYNSNHITLQLKSMLNSNKNIEDKEILNTFSILFGLIGLDPKDLSNFNKYLLEFLKNKENKKSIINFLNLFIDSKNLSKSFQDINNALNNAINKKSYAGLNFEEIKKIVYSTTTSKQNIYEILKEIFYNKSLRQDLKRSEYAKLFKAIFSDVFIKKILKRLLPQTTVDLFKNLFNNKDIEKGLDNIFQKVLNLLINNSDKYFKFEKIDDFITTLVHDSSKDVNDLVSLIIKEISKDSKLANYLTGKIVDSLYKSYDLDLLEKPNISLFVSTFFDNISNFDGLSQISNIIIQILMNSKLDDDHQNRDLISEILNGILFDKYDLNRDNSLLFKILSFEPKIQNINDYKQGLKHLALKYAIKNNALMPKNIGKLIDKDSRESILRFYKNIALNAKKNLNEFGSEIFKDIISLLVNELFKNNSLFSQLIDQIGNYVVIQPIVAFIKSINYKKIFNNEEEIQKFVENV", "aa_hexdigest": "558a085ee19f24852d0bed2f10fe502c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 82923.30680000044, "isoelectric_point": 8.943650627136233 }, "id": "DFOKDJOJIG_107", "locus": "GCA013367855_00067" }, { "type": "cds", "sequence": "contig_1", "start": 41947, "stop": 42315, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGTACAAAAATCCTCAAGGCGATTGTTGCTTTAGGAATTACATCTTCAATTGCTTCTAGTTCATTAGTTGCTATATCATGCAAAGACAAAACTAAAAAGCCGGATACAAAAATAACTCAAAGTGAAAATAAAGTTTCTAAAAAAATTAATTATGTTGCATTAGGCGACGGCTTTGCTGCAGGTTATAATAGCAGCTCAAATTACTATTTCAACAATGAATATGATAGCAAAAACGATTTAGTTTTAGGAACATCGTATCCTTCATACATTGCTAATTTTATAAAACTATTATCTGATAAACAAACAACATTATCAACTTATTATAATTACGGTTTGCCGCTTTCAAATGTTAATGATTGA", "aa": "MKKSTKILKAIVALGITSSIASSSLVAISCKDKTKKPDTKITQSENKVSKKINYVALGDGFAAGYNSSSNYYFNNEYDSKNDLVLGTSYPSYIANFIKLLSDKQTTLSTYYNYGLPLSNVND", "aa_hexdigest": "debf6fe0fcd68e5512917b80ea84e908", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13360.959999999986, "isoelectric_point": 9.320919609069826 }, "id": "DFOKDJOJIG_108", "locus": "GCA013367855_00068" }, { "type": "cds", "sequence": "contig_1", "start": 42325, "stop": 42879, "strand": "-", "frame": 1, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F3FC695", "UniRef:UniRef90_UPI001F3FC695" ], "nt": "ATGTCAACAAGATCAGCAGGTTTTTCAACTGTAAATTATTATAATTTTACTCAACCTACAATTATGGTTCATGGTATTTTAATGTTTATTGGATTTTCTCCTGTTTCAACTGCTGGAGGTATTCGTAATATTACAGTGGCTATAATTTTTATGAGTATAATTACTTTAATTAAAAATCGCAAAAACATAAATGCCTTTCATAGACAAATAGGAAAAGAATCTTTAATTAAGGCAGTTAACATTTTTGCAATTGCAATTTTTATAACTCTAATAATGACAATAGTAATATATCATACACTTCCAAGCGATTTAAAAAATGTTTATAATGAGTCATATCCAATGGTCTATGTAATGTTTGAAGTTTTCTCAGCATTCGGAAACTCAGGACTTAATATTGGATTAACACCAAATTTAGGTATTACTGGAAAAATACTTTTAATTATAACAATGATAGTAGGACAATTAGGAATATCACAATTTCTAAAAATTTTTACTTTTTCAAAATCAAAACCACAAAGATATCAATATATTTATGAAGATATTTCTATTGGTTAG", "aa": "MSTRSAGFSTVNYYNFTQPTIMVHGILMFIGFSPVSTAGGIRNITVAIIFMSIITLIKNRKNINAFHRQIGKESLIKAVNIFAIAIFITLIMTIVIYHTLPSDLKNVYNESYPMVYVMFEVFSAFGNSGLNIGLTPNLGITGKILLIITMIVGQLGISQFLKIFTFSKSKPQRYQYIYEDISIG", "aa_hexdigest": "f37022e26a30740f58d734d53459e704", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 42325, "stop": 43086, "observations": { "mutation": [ 42939 ], "stop": [ 42939 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F3FC695", "query_cov": 1.0, "subject_cov": 0.7215686274509804, "identity": 1.0, "score": 346.0, "evalue": 3.97e-119, "gene_start": 1, "gene_end": 184, "reference_start": 72, "reference_end": 255, "reference_sequence": "MTKIKSIKKDNPRHCFTLFTKFTIITYIFTSLIGLLLTFIFETTAKGANYFWNSSEYGSIGDKVFAIYFQTMSTRSAGFSTVNYYNFTQPTIMVHGILMFIGFSPVSTAGGIRNITVAIIFMSIITLIKNRKNINAFHRQIGKESLIKAVNIFAIAIFITLIMTIVIYHTLPSDLKNVYNESYPMVYVMFEVFSAFGNSGLNIGLTPNLGITGKILLIITMIVGQLGISQFLKIFTFSKSKPQRYQYIYEDISIG" }, "paralog": false, "identity": 0.9960474308300395, "score": 494.0, "evalue": 7e-180, "description": "Internal stop codon at 42939. Nonsense mutation around 42939.", "psc": { "uniref90_id": "UniRef90_UPI001F3FC695", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI001F3FC695", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F3FC695", "UniRef:UniRef50_UPI001F3FC695" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F3FC695", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F3FC695" ], "product": "potassium transporter TrkG" } }, "id": "DFOKDJOJIG_109", "locus": "GCA013367855_00069" }, { "type": "cds", "sequence": "contig_1", "start": 43090, "stop": 43263, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACTAAGATTTGGAATATTTCATACACTAAGCGCAATAAATAATGCTGGATTAGATATAATTGGCACAAAAAGTTTGCAACCTTATTATCATAATTATTACTTTTTATTTTTAACATCAATTTTATTTCTAATTGGTGGAATGGGATTCCCTGTATTCTATGATGTTTGA", "aa": "MSLRFGIFHTLSAINNAGLDIIGTKSLQPYYHNYYFLFLTSILFLIGGMGFPVFYDV", "aa_hexdigest": "aaf7ace7f181f8eeb3756d7d54f88553", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6498.521599999998, "isoelectric_point": 6.68991527557373 }, "id": "DFOKDJOJIG_110", "locus": "GCA013367855_00070" }, { "type": "cds", "sequence": "contig_1", "start": 43315, "stop": 43698, "strand": "-", "frame": 1, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F33CDF9", "UniRef:UniRef90_UPI001F33CDF9" ], "nt": "TTGCTATTAGCAGCCTCATCATTTACAACAACCGGTTTAAGTGGAGTTGCCGTTAGTGAAGTATTTAACATGTTTGGACAGATATTAATAGCTGTCTGTTTTATTCTTGGCGGAATTGGAATTTTTACATTTAAACTTTATATTTTTCAATCATTGCTTGGATTAAGAACGAATTTATTTAGTGGCCAAATTCACCAAACTGAAAGAGGCGGTTCTAATATTTATGAAACAAAAAAAATGATAAAAGTTTCAATAAGTTTTTTAATTATTATTTCTATATTATTTTCTTTAATATTTTCTTTAATATTTTATTATGATAATACAGCACTTTTTAGTGATGAAAAGATGTCTCAATTAAATGCATTAAGTAAAAATAATCCATGA", "aa": "MLLAASSFTTTGLSGVAVSEVFNMFGQILIAVCFILGGIGIFTFKLYIFQSLLGLRTNLFSGQIHQTERGGSNIYETKKMIKVSISFLIIISILFSLIFSLIFYYDNTALFSDEKMSQLNALSKNNP", "aa_hexdigest": "df4b2a49f6c7991871a4e907aea3c07b", "start_type": "TTG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "both", "pseudogene": { "start": 43014, "stop": 43755, "observations": { "insertion": [ 43086, 43111, 43157, 43191 ], "mutation": [ 43092, 43317, 43746 ], "stop": [ 43092, 43317, 43746 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F33CDF9", "query_cov": 1.0, "subject_cov": 0.5059760956175299, "identity": 0.992, "score": 215.0, "evalue": 1.26e-68, "gene_start": 1, "gene_end": 127, "reference_start": 20, "reference_end": 146, "reference_sequence": "MLYWEVSHNPSKHISYLDALLLAASSFTTTGLSGVAVSEVFNMFGQILIAVCFILGGIGIFTFKLYIFQSLLGLRTNLFSGQIHQTERGGSNIYETKKMIKVSISFLIIISILFSLIFSLIFYYDNTALFSDEKMSQLNALSKNNPWTFNPYEESIRNPYHNFGMSLRFGIFHTLSAINNAGLDIIGTKVCNLIIIIITFIFNINYISNWWNGIPVFYDVWQKLKASRKTILVIALHYLQNLQLLHTFLHH" }, "paralog": false, "identity": 0.9836734693877551, "score": 428.0, "evalue": 5.14e-155, "description": "Internal stop codon at 43092, 43317, 43746. Frameshift due to insertion around 43086, 43111, 43157, 43191. Nonsense mutation around 43092, 43317, 43746.", "psc": { "uniref90_id": "UniRef90_UPI001F33CDF9", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI001F33CDF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F33CDF9", "UniRef:UniRef50_UPI001F33CDF9" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F33CDF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F33CDF9" ], "product": "potassium transporter TrkG" } }, "id": "DFOKDJOJIG_111", "locus": "GCA013367855_00071" }, { "type": "cds", "sequence": "contig_1", "start": 44026, "stop": 44718, "strand": "+", "frame": 1, "gene": "trkA", "product": "Potassium uptake protein KtrA", "db_xrefs": [ "COG:COG0569", "COG:PT", "RefSeq:WP_012855311.1", "SO:0001217", "UniParc:UPI0001BC203B", "UniRef:UniRef100_D1J7H4", "UniRef:UniRef50_D1J7H4", "UniRef:UniRef90_D1J7H4" ], "nt": "ATGAGTTTATTTAAAAAGATACGTGAAATTTGTATTATTGGTATAGGTCGTTATGGTTCGTCGGTAGCAAGAACATTATTGGCCGATAGAAATAATAATATAAGGATAGTTTTAGTTGATGAAGATGAAAAACATTTGTTGCCTTTTAAAGATGAAGTAGAAATGATCTATGTTGCCGATTGTGCTGAACAAAAAACATTAGAAAGTCTTAATATAAAAGATTTTGATGCCGTTATTGTTGGCGCTTCAAATAATATTGAAATAGTTGCTGCTTTAACTGAATGTGGTGTAAAAACTATAATAGCAAGAGCTTCAACAAAAAGACATGCCCAAGTTTTAAAGCAAATTGGTGTTAGCTCAATTGTTTGTCCTGAAGAAGAAGCCGGAAAGAAAACTGCAATGTTAGTTGCAAACCCTGCGCTTGCATTATATTCAGAAAACATGGTTGAATTGCAAGACGGATTTGTTTCTGCTTCAATATATGTTAAAAACCCTGCTGTTGCAAACAAAAAAATTAGTGAATTAAATTTCAGAAATAAATACAATGTTTTAGTAACTATGATTAAGAGAGATAAACATTCATACATTCCTGAAGGTGATTTTGAAATAAAATTAGATGATTTAATAACTCTTATTGGCCAAATTGAAGATATTATTCAAGTTATTGAAATTTGTGCAATGAATGAAAAATAA", "aa": "MSLFKKIREICIIGIGRYGSSVARTLLADRNNNIRIVLVDEDEKHLLPFKDEVEMIYVADCAEQKTLESLNIKDFDAVIVGASNNIEIVAALTECGVKTIIARASTKRHAQVLKQIGVSSIVCPEEEAGKKTAMLVANPALALYSENMVELQDGFVSASIYVKNPAVANKKISELNFRNKYNVLVTMIKRDKHSYIPEGDFEIKLDDLITLIGQIEDIIQVIEICAMNEK", "aa_hexdigest": "18d450a60381e5c4899d963182ccef7d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC203B", "ncbi_nrp_id": "WP_012855311.1", "uniref100_id": "UniRef100_D1J7H4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC203B", "RefSeq:WP_012855311.1", "UniRef:UniRef100_D1J7H4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7H4", "uniref90_id": "UniRef90_D1J7H4" }, "psc": { "uniref90_id": "UniRef90_D1J7H4", "gene": "TrkA", "product": "Potassium uptake protein KtrA", "uniref50_id": "UniRef50_D1J7H4", "cog_id": "COG0569", "cog_category": "PT" }, "pscc": { "uniref50_id": "UniRef50_D1J7H4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H4" ], "product": "Potassium uptake protein KtrA" }, "genes": [ "ktrA", "trkA" ], "id": "DFOKDJOJIG_112", "locus": "GCA013367855_00072" }, { "type": "cds", "sequence": "contig_1", "start": 44829, "stop": 45038, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTTAGACAATGAGCTTCTTAAAAAATTAGAAGCAAAATACGAATCTAATTTAAATAACAAAATTATTGAAAATGCTATTTTTAAAAATGGTATTAAGGCTTCATCAACTAATAATCAAGTTGTAAGAAAACATGATTTTCAATTTTCTGTTGAAGTAAAACCTGGTTCAATAACTAACCAAAAACATTCAGGTAGATGTTGA", "aa": "MAILDNELLKKLEAKYESNLNNKIIENAIFKNGIKASSTNNQVVRKHDFQFSVEVKPGSITNQKHSGRC", "aa_hexdigest": "3609b43cf55c2d29729f13c1fda61b8a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7802.815200000001, "isoelectric_point": 9.463910484313967 }, "id": "DFOKDJOJIG_113", "locus": "GCA013367855_00073" }, { "type": "cds", "sequence": "contig_1", "start": 45075, "stop": 45167, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGATTTAAACATTGAAGATTTAGAATTTTCACAAAATTTTATTCATTTTTATGACAAATTGGAAAAAGCAAATGCATATCTAACATGA", "aa": "MKDLNIEDLEFSQNFIHFYDKLEKANAYLT", "aa_hexdigest": "189e783e2c094f0c78409ad88256c469", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3651.0591000000004, "isoelectric_point": 4.581245613098145 }, "id": "DFOKDJOJIG_114", "locus": "GCA013367855_00074" }, { "type": "cds", "sequence": "contig_1", "start": 45183, "stop": 45362, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTAGATAATGAAGATAGATTATTTAGAAGATATAATGAAAACCCTATTTCGGATGGTGGATATTTTGAATTCTTTTTAAATATTGCTAGCAAATATGGAATTGTTCCAAAATCTGCAATGCCAGAATCATTCCATAGTGAAGCAACAAACGATATGGTAGAACAAATTAATTGA", "aa": "MELDNEDRLFRRYNENPISDGGYFEFFLNIASKYGIVPKSAMPESFHSEATNDMVEQIN", "aa_hexdigest": "42c67e7c28df1faf8ac2490492c4e0f8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6878.514100000002, "isoelectric_point": 4.405101585388183 }, "id": "DFOKDJOJIG_115", "locus": "GCA013367855_00075" }, { "type": "cds", "sequence": "contig_1", "start": 45387, "stop": 45776, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGAAGCATATAAAAAGAATCATAATATGAGTGAAGTAGAAGCAATTCAACAACATGCTTTGGAAGACGTTTACAACATTTTAGTTAAGTCCCTAGGTATGCCTCCAAAAACATTTATTTTTGAATACTATGACAAAGATAAAAAATTTGTAAGATTGCCAGAAATGACACCAATAAAGTTTTTTGAAACATACATTAAACAATTTGTTGAAAATAAGGTTGATGTAATTAGCGACCCAAGAGAAATTTACCCTTACTATAGACAATTGGTTGCTCCTTCGGTTTTCAATTCGGTTGGTGGTATTGAATATAAAATGTTAAATATACCAATGAATGAATTAAAGGCAGCAGCGATTAAACAATTGCAATCTGGTCAATCAATTTGA", "aa": "MREAYKKNHNMSEVEAIQQHALEDVYNILVKSLGMPPKTFIFEYYDKDKKFVRLPEMTPIKFFETYIKQFVENKVDVISDPREIYPYYRQLVAPSVFNSVGGIEYKMLNIPMNELKAAAIKQLQSGQSI", "aa_hexdigest": "86e947b32f4a59983984df1705be817d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15117.370899999984, "isoelectric_point": 7.850975990295411 }, "id": "DFOKDJOJIG_116", "locus": "GCA013367855_00076" }, { "type": "cds", "sequence": "contig_1", "start": 46059, "stop": 46160, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATTGTTGACAAGGAATTTTTATCAAAAGAAGCAATTGAAGGTCTTTCAAAAGAAGCTATAGAAATTTCTCCATACGACCCATTATGTGAAGAATAA", "aa": "MLIVDKEFLSKEAIEGLSKEAIEISPYDPLCEE", "aa_hexdigest": "eb71dcd39b353ef260716d4fc5eb2555", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3740.2521000000006, "isoelectric_point": 4.216737174987793 }, "id": "DFOKDJOJIG_117", "locus": "GCA013367855_00077" }, { "type": "cds", "sequence": "contig_1", "start": 46274, "stop": 46417, "strand": "+", "frame": 2, "gene": "acpS", "product": "Holo-[acyl-carrier-protein] synthase", "db_xrefs": [ "COG:COG0736", "COG:I", "SO:0001217", "UniRef:UniRef50_D1J7H8", "UniRef:UniRef90_D1J7H8" ], "nt": "ATGTTAGGTGTGGACTTAACCGATATTAGACGATTTAAAGAATATAGTGAAACGCTTTTGATTAAGTTCTTACATCCGATTGAAATTGAACAATTTAAAAAGGTTTCGATAACAAAACGTGCTGATTTTCTTGCGACCAGATGA", "aa": "MLGVDLTDIRRFKEYSETLLIKFLHPIEIEQFKKVSITKRADFLATR", "aa_hexdigest": "c6a974de7e7b90d325ed84c9ef20712f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "acpS" ], "truncated": "3-prime", "pseudogene": { "start": 46274, "stop": 46601, "observations": { "insertion": [ 46565 ], "mutation": [ 46415 ], "stop": [ 46415 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7H8", "query_cov": 1.0, "subject_cov": 0.42727272727272725, "identity": 1.0, "score": 94.7, "evalue": 3.38e-24, "gene_start": 1, "gene_end": 47, "reference_start": 1, "reference_end": 47, "reference_sequence": "MLGVDLTDIRRFKEYSETLLIKFLHPIEIEQFKKVSITKRADFLATRWAIKEAIFKADNQYYSFNQINITKDEVGRYVFKDFEISTSNEGIYIIAVVLKDRTNSRSYLWI" }, "paralog": false, "identity": 0.9814814814814815, "score": 200.0, "evalue": 2.1e-68, "description": "Internal stop codon at 46415. Frameshift due to insertion around 46565. Nonsense mutation around 46415.", "psc": { "uniref90_id": "UniRef90_D1J7H8", "gene": "AcpS", "product": "Holo-[acyl-carrier-protein] synthase", "uniref50_id": "UniRef50_D1J7H8", "cog_id": "COG0736", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7H8", "UniRef:UniRef50_D1J7H8", "COG:COG0736", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7H8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H8" ], "product": "Holo-[acyl-carrier-protein] synthase" } }, "id": "DFOKDJOJIG_118", "locus": "GCA013367855_00078" }, { "type": "cds", "sequence": "contig_1", "start": 46598, "stop": 46861, "strand": "+", "frame": 2, "gene": null, "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AB411F", "UniRef:UniRef90_UPI0023AB411F" ], "nt": "ATGAATTTAAGAACAAGAATATTTTTTAGATTTTTTGGTTTGTTACATTGCATATCGTTTTTATGTGCAAAATCAAGACGCGATTTTAAAATGCCTGATTATTACAAAGTAGTAGAAAAAAACGACTTTTTGCAACAAAAAAGTAATGCCATTTTAAACCATTTTAATATTTCTGTTAATGTTGAAGGGTTTGAGAATGTCCCTAATGGACCATGTTTAATTATTCCAAACCATTCAACGTATTTAGATCCACTAATAATGTGA", "aa": "MNLRTRIFFRFFGLLHCISFLCAKSRRDFKMPDYYKVVEKNDFLQQKSNAILNHFNISVNVEGFENVPNGPCLIIPNHSTYLDPLIM", "aa_hexdigest": "e4346338aaa50bbc249342aa214d6162", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 46598, "stop": 47108, "observations": { "insertion": [ 47051 ], "mutation": [ 46859, 46871 ], "stop": [ 46859, 46871 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AB411F", "query_cov": 1.0, "subject_cov": 0.514792899408284, "identity": 1.0, "score": 182.0, "evalue": 2.14e-57, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MNLRTRIFFRFFGLLHCISFLCAKSRRDFKMPDYYKVVEKNDFLQQKSNAILNHFNISVNVEGFENVPNGPCLIIPNHSTYLDPLIMWSALWNHRDGSKQSKLVNFVARKEITKKKMVKRIADLGQTYYIDTQNPREALATLKDFGQFIKKINHAALFLLKEQEQKMEN" }, "paralog": false, "identity": 0.9822485207100592, "score": 330.0, "evalue": 5.55e-118, "description": "Internal stop codon at 46859, 46871. Frameshift due to insertion around 47051. Nonsense mutation around 46859, 46871.", "psc": { "uniref90_id": "UniRef90_UPI0023AB411F", "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "uniref50_id": "UniRef50_UPI0023AB411F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AB411F", "UniRef:UniRef50_UPI0023AB411F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AB411F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AB411F" ], "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "DFOKDJOJIG_119", "locus": "GCA013367855_00079" }, { "type": "cds", "sequence": "contig_1", "start": 46946, "stop": 47203, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAAAAGAATAGCGGATTTAGGTCAAACTTATTATATTGATACTCAAAACCCTAGAGAAGCATTGGCAACCTTGAAAGATTTCGGTCAATTTATTAAAAGAAATAAATCATGCGGCGTTATTTTTGCTGAAGGAACAAGAACAAAAGATGGAAAATTAGGCGAATTTCATAGTGGTGCCTTTAAACTTGCTCAATCTTCATATTTAACAATTGTTCCAGCAACAATAAATAATGCAGCAAACGCATTGGATTGA", "aa": "MVKRIADLGQTYYIDTQNPREALATLKDFGQFIKRNKSCGVIFAEGTRTKDGKLGEFHSGAFKLAQSSYLTIVPATINNAANALD", "aa_hexdigest": "c448ccf56ce16cd3480acbc017c0ec74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9311.48659999999, "isoelectric_point": 9.205908012390136 }, "id": "DFOKDJOJIG_120", "locus": "GCA013367855_00080" }, { "type": "cds", "sequence": "contig_1", "start": 47249, "stop": 47407, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16AA7C", "UniRef:UniRef90_UPI001F16AA7C" ], "nt": "ATGAAGCCAATTCAATTTCAATCACTTGATAGCCGTGTATTAGCAGAACAAGTTAAATCGATAGTTGCATCAGATTATATTGATCAAACAATTACATCAAATGAAACAATCAAAAACAAATATTCAAAACGAGACAAAGTAAAAGAAGTTAAGAGATAA", "aa": "MKPIQFQSLDSRVLAEQVKSIVASDYIDQTITSNETIKNKYSKRDKVKEVKR", "aa_hexdigest": "31d913204e2b4863eaf4ddd38e347b0c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 47195, "stop": 47407, "observations": { "mutation": [ 47201 ], "stop": [ 47201 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F16AA7C", "query_cov": 1.0, "subject_cov": 0.7428571428571429, "identity": 1.0, "score": 96.7, "evalue": 2.4e-25, "gene_start": 1, "gene_end": 52, "reference_start": 19, "reference_end": 70, "reference_sequence": "MDWNRKEKLEVKVTFHKPMKPIQFQSLDSRVLAEQVKSIVASDYIDQTITSNETIKNKYSKRDKVKEVKR" }, "paralog": false, "identity": 0.9714285714285714, "score": 134.0, "evalue": 1.08e-42, "description": "Internal stop codon at 47201. Nonsense mutation around 47201.", "psc": { "uniref90_id": "UniRef90_UPI001F16AA7C", "uniref50_id": "UniRef50_UPI001F16AA7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F16AA7C", "UniRef:UniRef50_UPI001F16AA7C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F16AA7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16AA7C" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6057.885300000001, "isoelectric_point": 9.629852104187012 }, "id": "DFOKDJOJIG_121", "locus": "GCA013367855_00081" }, { "type": "cds", "sequence": "contig_1", "start": 47411, "stop": 48235, "strand": "+", "frame": 2, "gene": "scpA", "product": "segregation/condensation protein A", "db_xrefs": [ "COG:COG1354", "COG:L", "KEGG:K05896", "RefSeq:WP_020002451.1", "SO:0001217", "UniParc:UPI0003713E10", "UniRef:UniRef100_UPI0003713E10", "UniRef:UniRef50_N9U9Z0", "UniRef:UniRef90_D1J7I0" ], "nt": "ATGTCCGATTTAGAAAAACCAATTATTAGAGAAATTTCAATAAAAGAAGAACCAAACAAAGATCAAAACAATCAAACAAATTCATCAATTTTTAAAGAAAAACACGTATTTAAATTAAAAAATTTTGATGGTCCACTTGATTTGTTGGTTTCTTTAATTAAAGAGAAAAATATAAGCATTTTTGATGTTGACTTATCTGAATTAGCAAATCAATATCTTGAAATAATTCATAGTCTTCAATCTTATGAAGTTGATATAGCAAGTGAATATCTAGTTATGGCTGCACATTTATTACAATTAAAAGCTAGAATGGTATTGCAAGATCCTGAAGTTGAAGAAGAAATTAAACAAGAAAAAAAGAGATTGCTTGAACAGATTGCCGAATATGAAAAATTCAAAGAAATTTCTTTACTTTTACGTGAACAAGAAGAAAAAAGATCTAGATTGTTTTCGAAAGAACCAGAAGACACACAAGATTTCGTAAGAGATATTGATGGTTCGATTCTTGATGGACATTCCAATAGTTCAAAATTAGTTGTAACTTTAAGAAAAATGTTTGAAAGAACATATGCTGAACTTATTAGAAATATTACTATTTCAACAATAGCAGTGAGTCCCGAAGAACAAAAAGAAAGAATTATTAAATTATTTAAAACAAAGTTGCAAGTAACCTTTAAAGATGTTTTTTCTGTTCCAAGTTTGGGACACTTTGTTATTACTTTATTGGCGGCATTGGATTTAGTAAGACAACAAATTATTGAAATTTATCAAGAAGAAAATGAAGGCGAAATTCATTTTAAGAAAGGGGCTTTATATGAAGAATAA", "aa": "MSDLEKPIIREISIKEEPNKDQNNQTNSSIFKEKHVFKLKNFDGPLDLLVSLIKEKNISIFDVDLSELANQYLEIIHSLQSYEVDIASEYLVMAAHLLQLKARMVLQDPEVEEEIKQEKKRLLEQIAEYEKFKEISLLLREQEEKRSRLFSKEPEDTQDFVRDIDGSILDGHSNSSKLVVTLRKMFERTYAELIRNITISTIAVSPEEQKERIIKLFKTKLQVTFKDVFSVPSLGHFVITLLAALDLVRQQIIEIYQEENEGEIHFKKGALYEE", "aa_hexdigest": "b496f58686bbbb029ae8479c24047e53", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0003713E10", "ncbi_nrp_id": "WP_020002451.1", "uniref100_id": "UniRef100_UPI0003713E10", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003713E10", "RefSeq:WP_020002451.1", "UniRef:UniRef100_UPI0003713E10" ] }, "ips": { "uniref100_id": "UniRef100_UPI0003713E10", "uniref90_id": "UniRef90_D1J7I0" }, "psc": { "uniref90_id": "UniRef90_D1J7I0", "gene": "ScpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_N9U9Z0", "cog_id": "COG1354", "cog_category": "L", "kegg_orthology_id": "K05896" }, "pscc": { "uniref50_id": "UniRef50_N9U9Z0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U9Z0" ], "product": "Segregation and condensation protein A" }, "genes": [ "scpA" ], "id": "DFOKDJOJIG_122", "locus": "GCA013367855_00082" }, { "type": "cds", "sequence": "contig_1", "start": 48225, "stop": 48857, "strand": "+", "frame": 3, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005515", "GO:0007059", "KEGG:K06024", "RefSeq:WP_175972173.1", "SO:0001217", "UniParc:UPI00158BE1A6", "UniRef:UniRef100_UPI00158BE1A6", "UniRef:UniRef50_Q6KHG8", "UniRef:UniRef90_D1J7I1" ], "nt": "ATGAAGAATAAAATTATTGAAGCTTTGCTATTTATTCAAGGCTCTGAAGGGTTAAGCAGTGAACAAGCACAACAAGTTTTCAAATTGAATACAATTCAAGAAGCAAGACGATTATTAAGGGATTTTAAAACATGCTTTAATAACTTGGAAAGAGGAATAAAAGTATTGGAATTCAATGATGTATTCAAGTTTTTAACTATTGAAAGCGTCAAAGATTATATTAGTGATTTAGTAACTATTACTAGAAAACAAAGATTATCAAATGCCGCAATTGAAGTTGCGGGTATTGTTGCTTATAAACAACCTATAACAAGAGGCATGATTTCAAACATAAGAGGTGTTGTAAGCGATGGAATTGTAAGTAGTTTATTAGAAAAAGGAATTATTGAAGAAGTTGGAGTAGCTCAAACTCCAGGCAACCCTATTTTATATGGAATTACTAACAAGTTCTATGATTATTTCAAAATAAAGACCTTAGGAGAATTGCCTCCTTTTCCTGAATTTAATAAATATAGCAGTGAAGAAGAGGAAGAAATTGATAAAGACTTCAATTTGTTTGATTCTCAAAGACCAGAAACGGACAATGATGGAGTTTTAATTTCTAATGAGGGGGCAGAATATGATAAATTTTAA", "aa": "MKNKIIEALLFIQGSEGLSSEQAQQVFKLNTIQEARRLLRDFKTCFNNLERGIKVLEFNDVFKFLTIESVKDYISDLVTITRKQRLSNAAIEVAGIVAYKQPITRGMISNIRGVVSDGIVSSLLEKGIIEEVGVAQTPGNPILYGITNKFYDYFKIKTLGELPPFPEFNKYSSEEEEEIDKDFNLFDSQRPETDNDGVLISNEGAEYDKF", "aa_hexdigest": "4167a60dcbe43e4da35f4243633bd5d8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158BE1A6", "ncbi_nrp_id": "WP_175972173.1", "uniref100_id": "UniRef100_UPI00158BE1A6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158BE1A6", "RefSeq:WP_175972173.1", "UniRef:UniRef100_UPI00158BE1A6" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158BE1A6", "uniref90_id": "UniRef90_D1J7I1" }, "psc": { "uniref90_id": "UniRef90_D1J7I1", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_Q6KHG8", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0007059" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHG8" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "DFOKDJOJIG_123", "locus": "GCA013367855_00083" }, { "type": "cds", "sequence": "contig_1", "start": 48844, "stop": 49677, "strand": "+", "frame": 1, "gene": "rluA", "product": "Ribosomal large subunit pseudouridine synthaseC", "db_xrefs": [ "COG:COG0564", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A448ZXK9", "UniRef:UniRef90_D1J7I2" ], "nt": "ATGATAAATTTTAAGGCTAAAAAGGATGACGAAGGTCGTATCCTTTTTAAATATTTGGTAAAGGTTTGCGATAATGTTCCAATATCTAGAATAGAGCGACTTTTTAGAGAAAAGGATATCAAGGTAAATAATATACGTGTAAATGATAAGCAATACAAAGTCAAAGAAGGAGATTTAATTTCAATTTATGGATTAATTGAAACATCGAAACATATTGACAACAAAGCTAATACAAACATAAATTTTAAAGTTATTTACGAAGATAAAAATCTTTTAATTATAGACAAGGCGCCAAATATTGCAATGCATTCTGAAGAAAATTGTTTAGATGATCAAGTTTTAAAATATTTAAATTTTAAAAAAGTTTCTTCGTTTAGACCTGCTTCAATTGGCAGAATAGACAAAAAAACTAGCGGTTTGGTTGTTTATGCAAAAAACTACAAAACTCTAGTTGAATTTGAAAATAAACAAAATAAATTTGACAAAATTTACCAATTTGTAAGTGATTTTGATGAAGATGTTTTGGATATAACAGTAAGACTAAAAAAAGATATTAAAAACGAAAAAATGATTGTCGATAATAATTTTGGAGTTAAAGCACAGACAATATTTTATAAAGAAGGAAATAGAAAATTTGCCAAGATAATAACAGGCAAAAAACATCAAATTAGGGCGACATTAGCATATTTAAAAACACCTATTTTAGGAGATATTAAATACGGCGGAAAATTTGCTAAAAGAATGTATTTGCATTCATATTCAATAAAATTTCATGATCTTTCTGATGATTTTAAAGAATATAATGAACAAGTATTCATTTGCTATCCAAAATGA", "aa": "MINFKAKKDDEGRILFKYLVKVCDNVPISRIERLFREKDIKVNNIRVNDKQYKVKEGDLISIYGLIETSKHIDNKANTNINFKVIYEDKNLLIIDKAPNIAMHSEENCLDDQVLKYLNFKKVSSFRPASIGRIDKKTSGLVVYAKNYKTLVEFENKQNKFDKIYQFVSDFDEDVLDITVRLKKDIKNEKMIVDNNFGVKAQTIFYKEGNRKFAKIITGKKHQIRATLAYLKTPILGDIKYGGKFAKRMYLHSYSIKFHDLSDDFKEYNEQVFICYPK", "aa_hexdigest": "c1f3f7b26def32bffe37a759dc27f785", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_D1J7I2", "query_cov": 1.0, "subject_cov": 0.9964028776978417, "identity": 1.0, "score": 547.0, "evalue": 7.88e-197, "valid": true, "gene": "RluA", "product": "Ribosomal large subunit pseudouridine synthaseC", "uniref50_id": "UniRef50_A0A448ZXK9", "cog_id": "COG0564", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXK9" ], "product": "Ribosomal large subunit pseudouridine synthase C" }, "genes": [ "rluA" ], "id": "DFOKDJOJIG_124", "locus": "GCA013367855_00084" }, { "type": "cds", "sequence": "contig_1", "start": 49700, "stop": 50002, "strand": "+", "frame": 2, "gene": "gatC", "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C", "db_xrefs": [ "COG:COG0721", "COG:J", "RefSeq:WP_012855320.1", "SO:0001217", "UniParc:UPI0001BC2044", "UniRef:UniRef100_D1J7I3", "UniRef:UniRef50_D1J7I3", "UniRef:UniRef90_D1J7I3" ], "nt": "ATGGAAAAAGAAGAGATTAAAAAATTGGCTAATGATCTTTTATTTGAACCAAGTGAAGAAGTTATTAAATTAACTAATAACTTGCTTAATTCAATAGAAAAAGGTTTAAAAGATTTAGAGGAATTTGACTTACAAGACATTAAACCGATGTCGCACATCAATGAATCACCCATTTGTTTTGAACAACTAAGAAGCGATGAACCAGATAAAAGTTTTTATTTGAATAAACAAGATTTATTAAATAATGCATCTGAACATGATGAAAATTATGTAATTATGAAGAAGGTAATAAATGATAAATAA", "aa": "MEKEEIKKLANDLLFEPSEEVIKLTNNLLNSIEKGLKDLEEFDLQDIKPMSHINESPICFEQLRSDEPDKSFYLNKQDLLNNASEHDENYVIMKKVINDK", "aa_hexdigest": "e5f45cfef76f2cb5e32c174fb203ef47", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2044", "ncbi_nrp_id": "WP_012855320.1", "uniref100_id": "UniRef100_D1J7I3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2044", "RefSeq:WP_012855320.1", "UniRef:UniRef100_D1J7I3" ] }, "ips": { "uniref100_id": "UniRef100_D1J7I3", "uniref90_id": "UniRef90_D1J7I3" }, "psc": { "uniref90_id": "UniRef90_D1J7I3", "gene": "GatC", "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C", "uniref50_id": "UniRef50_D1J7I3", "cog_id": "COG0721", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J7I3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I3" ], "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C" }, "genes": [ "gatC" ], "id": "DFOKDJOJIG_125", "locus": "GCA013367855_00085" }, { "type": "cds", "sequence": "contig_1", "start": 49992, "stop": 50894, "strand": "+", "frame": 3, "gene": "gatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "db_xrefs": [ "COG:COG0154", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A449AI30", "UniRef:UniRef90_A0A6A8PZD5" ], "nt": "ATGATAAATAAAGGTAATTTAAAAGAAGCAATTTTACAATTAAATGCTGATAAAAATAATGCGGTATCATTTGTTTTTAACGATGCTCAATTAAAAGAACAAGGACCGCTTGCAAATTGTGTTTTTACAATTAAGGACAATTATGCAGATTTGAATGTTATTGAAAGCGCTTCAAGCAGATTTCTTTCTAATTTTAGACCTTCATATCAATCTACAATCTTAAAATTATTAAATGAAGCAGGAGCAACGTGCGTTGCAAGAACGAATTTAGATGAATTTGGTATGGGTGGCAGTGGAGAATATTCATGCAATGGATTAATAAAAAATCCTTTGAATAGTAATTATTTAGTTGGAGGTTCTTCATCAGGTGCCGCTGCAACTTTTTCTAAAAATATCTCGTTTGCAATTGGATCTGACACAGGCGATTCAGTTAGAAAACCTGCATCAAATATTGGTCAGGTTGGATTTAAGCCAAGTTATGGCGCCATAAGCCGTTATGGATTGTCTGCTTTTGCTTCGTCATTAGATACAGTTGCATATTTTACACATAATGTTCAAGATGCAATAACTCTCTCATCAGTATTATTTAAGCAAGATTTAAAACATGATTTGACATCAGTTGATTTGTCGTTTAATGCAGATAGTGTCATTGAAACAAAACCACATAAAATTGCTTATTTGGATTGTTTTGACAAATTGGATATATCTGTTGCTGATGCATATAAAAAATTATTGAATAAGCTAGAAAAAATGAATATTGAACTTGTAAAAATTTCAATAAATGAAAAGTTACTGCAAGCCATCGATACTGTTTATAAGGTTATTGCTTTTTCAGAATCTTCATCTAATTTAGCAAATCTTGTAGGTGTTGAATTTGGCAATAGAATTACCGGCAATTCTTGA", "aa": "MINKGNLKEAILQLNADKNNAVSFVFNDAQLKEQGPLANCVFTIKDNYADLNVIESASSRFLSNFRPSYQSTILKLLNEAGATCVARTNLDEFGMGGSGEYSCNGLIKNPLNSNYLVGGSSSGAAATFSKNISFAIGSDTGDSVRKPASNIGQVGFKPSYGAISRYGLSAFASSLDTVAYFTHNVQDAITLSSVLFKQDLKHDLTSVDLSFNADSVIETKPHKIAYLDCFDKLDISVADAYKKLLNKLEKMNIELVKISINEKLLQAIDTVYKVIAFSESSSNLANLVGVEFGNRITGNS", "aa_hexdigest": "f9eb44fe53d6e1e31da979b6d9cf7d49", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "gatA" ], "truncated": "3-prime", "pseudogene": { "start": 49992, "stop": 51197, "observations": { "mutation": [ 50892 ], "stop": [ 50892 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8PZD5", "query_cov": 1.0, "subject_cov": 0.6787330316742082, "identity": 0.997, "score": 567.0, "evalue": 1.34e-201, "gene_start": 1, "gene_end": 300, "reference_start": 1, "reference_end": 300, "reference_sequence": "MINKGNLKEAILQLNADKNNAVSFVFKDAQLKEQGPLANCVFTIKDNYADLNVIESASSRFLSNFRPSYQSTILKLLNEAGATCVARTNLDEFGMGGSGEYSCNGLIKNPLNSNYLVGGSSSGAAATFSKNISFAIGSDTGDSVRKPASNIGQVGFKPSYGAISRYGLSAFASSLDTVAYFTHNVQDAITLSSVLFKQDLKHDLTSVDLSFNADSVIETKPHKIAYLDCFDKLDISVADAYKKLLNKLEKMNIELVKISINEKLLQAIDTVYKVIAFSESSSNLANLVGVEFGNRITGNSWEETFNLSRTEGIGKMVQSRMILGSYFLEDNNQIKYFVRAKKMRRYLSNYFTNVHKSADIFIYPASNMSAPLIGKLYEPSYMDYILTNSNLTGNPSLTLKLGIDKNNNLPFNLAIDGEIYKDCKMLSHALFIERVLEGEKNE" }, "paralog": false, "identity": 0.9875311720698254, "score": 773.0, "evalue": 2.72e-285, "description": "Internal stop codon at 50892. Nonsense mutation around 50892.", "psc": { "uniref90_id": "UniRef90_A0A6A8PZD5", "gene": "GatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "uniref50_id": "UniRef50_A0A449AI30", "cog_id": "COG0154", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8PZD5", "UniRef:UniRef50_A0A449AI30", "COG:COG0154", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AI30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AI30" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A" } }, "id": "DFOKDJOJIG_126", "locus": "GCA013367855_00086" }, { "type": "cds", "sequence": "contig_1", "start": 50937, "stop": 51320, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "ATGGTTCAATCAAGAATGATTTTAGGATCTTATTTTTTAGAAGATAATAATCAAATAAAGTATTTTGTTAAGGCTAAAAAAATGAGAAGGTATTTAAGTGATTATTTCACTAATGTTCATAAAAGCGCAGATATATTTATCTATCCAGCATCTAACATGTCCGCTCCTCTTATTGGAAAATCATATGAACCAAGTTATATGGATTATATTTTAACAAATTCTAATTTGACAGGAAATCCTTCTTTGACATTAAAATTAGGAATTGACAAAAATAATAATCTTCCATTTAATTTAGCAATTGACGGAGAAATATATAAAGATTGCAAGATGCTAAGCCACGCATTATTTATTGAAAGAGTATTGGAAGGTGAAAAAAATGAATAA", "aa": "MVQSRMILGSYFLEDNNQIKYFVKAKKMRRYLSDYFTNVHKSADIFIYPASNMSAPLIGKSYEPSYMDYILTNSNLTGNPSLTLKLGIDKNNNLPFNLAIDGEIYKDCKMLSHALFIERVLEGEKNE", "aa_hexdigest": "f1825d3a44d9743c382b929c3189e9c7", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 119, "aa_cov": 0.8661417322834646, "hmm_cov": 0.9915966386554622, "evalue": 1.7769070934615035e-10, "score": 36.06938552856445, "start": 2, "stop": 111 } ], "seq_stats": { "molecular_weight": 14632.675399999984, "isoelectric_point": 7.782832908630372 }, "id": "DFOKDJOJIG_127", "locus": "GCA013367855_00087" }, { "type": "cds", "sequence": "contig_1", "start": 51379, "stop": 51657, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "ATGTTTTCTCCAGTTCAAAATGAATACGGAGATGAAGCCAATACAAATGTTTCAAATATTGACCTTGCCTATCCTGGAAGTTTGCCTCTTTTAAATTCTAGTGCCATAATTAAGGCAATTAAATTAGCTAAGGCGCTAGAAATGGAAATAGACCCATTAGTTAGATTTGACAGGAAAAATTATTTTTATCCCGATTTAACAAAAGGATATCAAATTACTCAACAATTTCATCCAATTGGTAAAAATGGTTTGATAAGAGCAAAGGTTCAAAATTCATGA", "aa": "MFSPVQNEYGDEANTNVSNIDLAYPGSLPLLNSSAIIKAIKLAKALEMEIDPLVRFDRKNYFYPDLTKGYQITQQFHPIGKNGLIRAKVQNS", "aa_hexdigest": "6ba59d61015d10e89bd2db2e241135a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 90, "aa_cov": 0.9782608695652174, "hmm_cov": 1.0, "evalue": 7.214304699497972e-33, "score": 109.54290008544922, "start": 1, "stop": 90 } ], "seq_stats": { "molecular_weight": 10344.70249999999, "isoelectric_point": 7.964891624450684 }, "id": "DFOKDJOJIG_128", "locus": "GCA013367855_00088" }, { "type": "cds", "sequence": "contig_1", "start": 51685, "stop": 52734, "strand": "+", "frame": 1, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "COG:COG0064", "COG:J", "EC:6.3.5.6", "EC:6.3.5.7", "GO:0016884", "KEGG:K02434", "SO:0001217", "UniRef:UniRef50_A0A449AA70", "UniRef:UniRef90_D1J7F4" ], "nt": "ATGGAAGAAGATACTGCTAAATCAATTCACGAAAATGGTTTGACATATTTAAACTATAATAGATGTGGAGTTCCTTTAATTGAAATAGTTTCTAATCCTGTAATGCACAGCGCAGAGGATGCAATGGCTTACGTTGAAGCTATAAGACAAACAGCATTAGTTTTAGACATTAGTGATGCAAAATTAAATGAAGGAAGTTTAAGAACTGACGTTAATTTATCAGTAAGAAGAAAAGGAGAAAACATCCTTAATACTCGTACGGAAATAAAAAATTTAAATTCTCTTTCAAATATTAAAAAAGCAATTGAATATGAATCTAATTTCCAAATTCAAATGTATGAAAACAATTTAACTTTTAATCAAGAAACAAAGCGTTTTGATGAAAAAATGCAAAAGACTGTTTCAATGCGTTCAAAAGGTGATGCAATTAGTTACAAATATTTTCCAGATCCAAATTTACCTTATATTCAATTGCAACAAAAATTTATAGATGATATTAAAATAGAAGAAATACCATATCAAAAAGAACTAAGATATCTAAAAAATGGCTTAAATCAAGTTCAAATATCTCAATTGTTAAATAATATTTCTTATGCAAAATATATTGATAGTATAAACTCAAAAGATTTTAAAAAAGCCACAAATGTTTTCTTTTCTGACATAGTTACCTATTTAAATAGTAACAATAAGAATATTGAAAATTTATCATTTAAAGCAGATGATGCGAGAAAGGTTGTCGAATATTTAATTGATAATAGGATTAATAAAAATAATATTGCTGAAATTATCAAAATTAAAGATCAAAATCCAAATTTAAATATTGAATCTATTATTAAAGACAATGGCTTTTTAATTGAAATAAAAGAAATTGATTTGAACGAACTAGTTCAAGAAATATTAAATGAAAATCCAAACTTGGAAACTGAATTTAAAAACAATTATAACCGTGCCCAAAAATTTATGCAAGGTCAAATAATGAAAAAAACAATGGGAAAGGCAAATCTAAAAAATCTTGATGAAATAATCAAAAATAGGTTCAATAATGAGTAA", "aa": "MEEDTAKSIHENGLTYLNYNRCGVPLIEIVSNPVMHSAEDAMAYVEAIRQTALVLDISDAKLNEGSLRTDVNLSVRRKGENILNTRTEIKNLNSLSNIKKAIEYESNFQIQMYENNLTFNQETKRFDEKMQKTVSMRSKGDAISYKYFPDPNLPYIQLQQKFIDDIKIEEIPYQKELRYLKNGLNQVQISQLLNNISYAKYIDSINSKDFKKATNVFFSDIVTYLNSNNKNIENLSFKADDARKVVEYLIDNRINKNNIAEIIKIKDQNPNLNIESIIKDNGFLIEIKEIDLNELVQEILNENPNLETEFKNNYNRAQKFMQGQIMKKTMGKANLKNLDEIIKNRFNNE", "aa_hexdigest": "2293b30236804f2bc68bd97cc41a5fb7", "start_type": "ATG", "rbs_motif": null, "genes": [ "gatB" ], "truncated": "5-prime", "pseudogene": { "start": 51385, "stop": 52734, "observations": { "mutation": [ 51655 ], "stop": [ 51655 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F4", "query_cov": 1.0, "subject_cov": 0.7378435517970402, "identity": 0.9940000000000001, "score": 662.0, "evalue": 1.06e-237, "gene_start": 1, "gene_end": 349, "reference_start": 125, "reference_end": 473, "reference_sequence": "MNNDWEIVIGIEIHIELNTKTKMFSPIQNEYGDEANTNVSNIDLAYPGSLPLLNSSAIIKAIKLAKALEMEIDPLVRFDRKNYFYPDLTKGYQITQQFHPIGKNGLIRAKVQNSWKTFSIERIHMEEDTAKSIHENGLTYLNYNRCGVPLIEIVSNPVMHSAEDAMAYVEAIRQTALVLDISDAKLNEGSLRTDVNLSVRRKGENVLNTRTEIKNLNSLSNIKKAIEYESNFQIQMYENNLTFNQETKRFDEKMQKTVSMRSKGDAISYKYFPDPNLPYIQLQQKFIDDIKIEEIPYQKELRYLKNGLNQVQISQLLNNISYAKYIDSINSKDFKKATNVFFSDIVTYLNSNNKNIENLLFKADDARKVVEYLIDNRINKNNIAEIIKIKDQNPNLNIESIIKDNGFLIEIKEIDLNELVQEILNENPNLETEFKNNYNRAQKFMQGQIMKKTMGKANLKNLDEIIKNRFNNE" }, "paralog": false, "identity": 0.9910913140311804, "score": 878.0, "evalue": 0.0, "description": "Internal stop codon at 51655. Nonsense mutation around 51655.", "psc": { "uniref90_id": "UniRef90_D1J7F4", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.6", "6.3.5.7" ], "uniref50_id": "UniRef50_A0A449AA70", "cog_id": "COG0064", "cog_category": "J", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0016884" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F4", "EC:6.3.5.6", "EC:6.3.5.7", "UniRef:UniRef50_A0A449AA70", "COG:COG0064", "COG:J", "KEGG:K02434", "GO:0016884" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AA70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AA70" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" } }, "id": "DFOKDJOJIG_129", "locus": "GCA013367855_00089" }, { "type": "cds", "sequence": "contig_1", "start": 52879, "stop": 53319, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCTTTAATTCTCGATTTTCACACTCTGAATGGAGATTTGTGTTCATATAAAGCATCTATTGAAAAAATAAATTTTGAATATCCCTTCGATGCCAAAATTAATACTGATAGCGCAAATAAGATTAAAGCAATTGCATTGCCTGTAGAACCTCATAACGTACTAAATGATGGAATTACTTCTGAAATTCCGGGAATAATTTGTCCCAAAATTACATTCTTTAGCACCACTTCATACTTTTCATCAACCGATCCAATAATTCCAAGCATCAATATTGAGATTGGAATAAAAGACAAAAATAGATACATTGCATATCCAGCAGGGACAAAAGAAAATTCATTTGAATTTATTTTTTCATATGCAGAATTTACAATTTCTTTTCCTTTTGTTTTTGAACTTTTAATAAATTTAGGTATTGCTATAAAAATAATTCCGTAA", "aa": "MNPLILDFHTLNGDLCSYKASIEKINFEYPFDAKINTDSANKIKAIALPVEPHNVLNDGITSEIPGIICPKITFFSTTSYFSSTDPIIPSINIEIGIKDKNRYIAYPAGTKENSFEFIFSYAEFTISFPFVFELLINLGIAIKIIP", "aa_hexdigest": "eded02bd57117ba86969b2e3e9f5251f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16332.639599999991, "isoelectric_point": 4.9170503616333 }, "id": "DFOKDJOJIG_130", "locus": "GCA013367855_00090" }, { "type": "cds", "sequence": "contig_1", "start": 53339, "stop": 53485, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTGTCTTTAATTTTACTAGGGCTAACAGCCTTTATGGTATTTTTAATTGATAAAGTAGGACTTGGAAAATTTGATGCCGATATTAAAAAAATGAAATTAGCAGATTTTAATTTAACTACAGAATTTTATTTAATATATTGA", "aa": "MILSLILLGLTAFMVFLIDKVGLGKFDADIKKMKLADFNLTTEFYLIY", "aa_hexdigest": "34a95c0f3e2425c725d584f3efb8db5a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5488.648999999999, "isoelectric_point": 5.955214500427247 }, "id": "DFOKDJOJIG_131", "locus": "GCA013367855_00091" }, { "type": "cds", "sequence": "contig_1", "start": 53498, "stop": 53809, "strand": "+", "frame": 2, "gene": null, "product": "YhjD/YihY/BrkB family envelope integrity protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE35A95", "UniRef:UniRef90_UPI002FE35A95" ], "nt": "TTGTTTTTACCTGTATTTACTTATTTAGGATTTCTATTTTATTTTGTATATGCCCCAAATTTTAAATTGAAAATGTCGCAAGCACATCCCGGGGCTTTGATAGCAGCAATACCAACAGCAATATTTATTTTGATTTTTGGTTCGTTAACATCCTTAATTCCTTATAAAAAATTTGGGATAGTTGCTTCATTTATGTATATTATTTTATTATTGAGTTTTACTTCTTATTTTATTTACATGGGAATTATTGTTAATTCAAGTTTTTATAAAACTTTTATTAATTTACCAACTGTAGAAAAACATTCATTTTGA", "aa": "MFLPVFTYLGFLFYFVYAPNFKLKMSQAHPGALIAAIPTAIFILIFGSLTSLIPYKKFGIVASFMYIILLLSFTSYFIYMGIIVNSSFYKTFINLPTVEKHSF", "aa_hexdigest": "f4d5323e4ec5f05e3006e13736be685c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 53200, "stop": 53889, "observations": { "insertion": [ 53248, 53804, 53817 ], "stop": [ 53294, 53483 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE35A95", "query_cov": 1.0, "subject_cov": 0.42738589211618255, "identity": 0.99, "score": 194.0, "evalue": 7.69e-61, "gene_start": 1, "gene_end": 103, "reference_start": 113, "reference_end": 215, "reference_sequence": "MRFYRQCNCFNLICAIGINFSIEGIFKIYFSIDALYEHKSPFRAWKSRIKGFIVSVFVCLILSLILLGLTAFMVFLIDKVGLGKFDADIKKMKLADFNLTTEFYLIYWFTTILFLPVFTYLGFLFYFVYAPNFKLKMSQAHPGALIAAIPTAIFILIFGSLTSLIPYKKFGIVASFMYIILLLSFTSYFIYMGIIVNSSFYKTFINLPTVEKHSFWKNKNFNLKSFDFFANFSLFIVINDI" }, "paralog": false, "identity": 0.9692982456140351, "score": 416.0, "evalue": 1.5e-150, "description": "Internal stop codon at 53294, 53483. Frameshift due to insertion around 53248, 53804, 53817.", "psc": { "uniref90_id": "UniRef90_UPI002FE35A95", "product": "YhjD/YihY/BrkB family envelope integrity protein", "uniref50_id": "UniRef50_UPI002FE35A95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE35A95", "UniRef:UniRef50_UPI002FE35A95" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE35A95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE35A95" ], "product": "YhjD/YihY/BrkB family envelope integrity protein" } }, "id": "DFOKDJOJIG_132", "locus": "GCA013367855_00092" }, { "type": "cds", "sequence": "contig_1", "start": 54029, "stop": 54778, "strand": "+", "frame": 2, "gene": null, "product": "ECF transporter S component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I4", "UniRef:UniRef90_D1J7I4" ], "nt": "GTGGGTGTTTTGATTGCTATCTCAGTTGTCTTTTTTCTAATAAGTGTTAGAATCATGCCCATAAGTGCCTTACCAAGTATAAAATTTAGCTTCATTGGTTTGCCAATAAAAATAACTGGTTTTATTTTTGGACCAATTGTAGGTATGATAACTGGGATATTGGCGGATATAATTTCATTTGCTCTAGTTCCAACTTACTATAATGTTTTGTATACAATAGCGGTTGCGGTATCAGGATTTGTTCCCGGGTTTGCTGCGTACTATTTTTTCAATATTAATGAATTGTTTTTTTCAAGAACGTATAGAATTTTTAAATACAAAGAAACAATTGATTATTTTAAAGAACAATATGATATTGCACTTACAATAGGTAACTCTGAAGATATGCAATATTTTTCAGAAAAAATTGCTTTTTATGAAGTCAAGATCATAATTTTAGAATGTAAGAAAAAGCCAATTGCCATGATTAATTTTTCATTTATTTCAACATTGATAATATTGGGTATTCAAGTGTTGATAATTCTTGCAATTTTTTCTAACCTAGATCCTTCAATATTTGAAAAAAATAGATTTGTTAAAAACAAAAAATTCTATATGTTCTTAACTTCTATAGGATTTATAGGAATGATGATTTTTGTTTCTCTATATAGAATATTTTTAAAGAAAAAATTTGAAACCTTTTTAGAAATAATGGCAATAATTTCATTTAGCGCTGTTTTAGAAATAGTAAATGTTATCTTATTGGCGTGA", "aa": "MGVLIAISVVFFLISVRIMPISALPSIKFSFIGLPIKITGFIFGPIVGMITGILADIISFALVPTYYNVLYTIAVAVSGFVPGFAAYYFFNINELFFSRTYRIFKYKETIDYFKEQYDIALTIGNSEDMQYFSEKIAFYEVKIIILECKKKPIAMINFSFISTLIILGIQVLIILAIFSNLDPSIFEKNRFVKNKKFYMFLTSIGFIGMMIFVSLYRIFLKKKFETFLEIMAIISFSAVLEIVNVILLA", "aa_hexdigest": "9db49f7cfba2e9009cbeac8f6e97cb26", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 53930, "stop": 54934, "observations": { "mutation": [ 53969, 54005, 54776 ], "stop": [ 53969, 54005, 54776 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I4", "query_cov": 0.9959839357429718, "subject_cov": 0.7425149700598802, "identity": 0.992, "score": 430.0, "evalue": 3.97e-150, "gene_start": 2, "gene_end": 249, "reference_start": 35, "reference_end": 282, "reference_sequence": "MNKLPGGLRHYIYWARSQTNISYRKWNSKRIAFVGVLIAISVVFFLISVRIMPISALPSIKFSFIGLPIKITGFIFGPIVGMITGILADIISFALVPTYYNVLYTIAVAVSGFVPGFAAYYFFNINELFFSRTYRIFKYKETIDYFKEQYDIALTIGNSEDMQYFSEKIAFYEVKIIILECKKKPIAMINFSFISTLIILGIQVLIILAIFSNLDPSIFEKNRFVKNKKFYMFLTSIGFIGMMIFVSLYRIFLKKKFETFLEIMAIISFSAVLEIVNVILLAWADTSTLKTDFWVNFTAQSLFSPVKIFFNLAIILATYKVVAPLIKSKEGGHL" }, "paralog": false, "identity": 0.9910179640718563, "score": 624.0, "evalue": 3.88e-229, "description": "Internal stop codon at 53969, 54005, 54776. Nonsense mutation around 53969, 54005, 54776.", "psc": { "uniref90_id": "UniRef90_D1J7I4", "product": "ECF transporter S component", "uniref50_id": "UniRef50_D1J7I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I4", "UniRef:UniRef50_D1J7I4" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I4" ], "product": "ECF transporter S component" } }, "id": "DFOKDJOJIG_133", "locus": "GCA013367855_00093" }, { "type": "cds", "sequence": "contig_1", "start": 54934, "stop": 55344, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAGCAATAAGTTCTTTAAAAGTTACCAATTTAAAAAGTATTAATGTTGTAAATGAACAATTTAAAAACTTAATTAATAATCTATCTTTTACACTTAATGCAAACAATATATTGGGTTTTGTAGGTGGAGATAGTGAAACAAATAATTTAATATTTAATTATTTAACTAATAATACAATAAATCCTAAAAGATATCAAGGCTACATTGAATTTCAAACAAACTTAGGTGAATATTCTTCAATATATAAGAACTCAAATTATCAAAATAACATAGCTTTTGGTTTTTATAATGATATTTTAAACAATGATTTAAATGAAGATAATAGCTATAGAAATATAAAAAAATATATTAACCACGGAGCAGTTATAGGGGCACTAACGGAAGAGTTTGTTTCTCAATGA", "aa": "MKKIAISSLKVTNLKSINVVNEQFKNLINNLSFTLNANNILGFVGGDSETNNLIFNYLTNNTINPKRYQGYIEFQTNLGEYSSIYKNSNYQNNIAFGFYNDILNNDLNEDNSYRNIKKYINHGAVIGALTEEFVSQ", "aa_hexdigest": "7074d2209fe7d3c109b94223dda4f3ea", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15508.084699999981, "isoelectric_point": 6.56259593963623 }, "id": "DFOKDJOJIG_134", "locus": "GCA013367855_00094" }, { "type": "cds", "sequence": "contig_1", "start": 55372, "stop": 56289, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E424311", "UniRef:UniRef90_UPI001E424311" ], "nt": "TTGCTTCAAAGTGAATTTGCCAAATTGCAATATGGTGTCGAAATTGAACTCGGAAGTAAATTAGACGACTTATTCAGTGAATTAAATCAATTTGATTTGCGAAATATCGACGCTAAAAATTTAAAATTTGATACTCTTTTTAAGAAATTTAAAGATATAGAAATTTTATATAGAACCCATGAAATGAAACTATTCGCATTTGTTAATGCAACTATAGCAAAATATAATAAGGGGTTATCGTTTTTAAAACGCAAGGAATATTTAGACAGTAAAAAGGAATACGAAGAGGTAATAAAAAAAATTTATAAGCCCTTTTCAAAGCCTAATAGATTTTTAAATCGAGTAAGATTTTTTTCTAAACTTTTAGAAACAAAAAAATCTTCAAAATCATCTATTAAAAAATTTGTAAATAAATTAAGGCGTGATTTAAATATTGAAAACTATTATTACAAATATGAATTAAAGAAAACAATAAAACAATCTTCGTTTATTCACTTTTATAAAAATCTATATTTTAATGGTCTATTATTGAAATTTATAAATAAAAATTTAAAGGCCATATTAGGGCTAAAAGAAGCAAAATCGTTTGAATTATTGTATGAAATTTTTGATTTAAAAAACAATATTACAAATGAAGTTAATGTAATTTCAAACATTGATAATCAAAAAGAATTAAAGAATGAAATAAAGGCTGTAATAAAAAATAATTTTTTAAATGATGTGATGCCATATATTGAAAGAATTAAGGCGTCTAGTTATGAACACACTCAAGAAGTTATTAATGATGTTACAAGAGAAATTCAAACGTCTAATAACTACGATTTAAACATAGGCGAGTTTGGCCTTGCAAACAAAGCGTTTGAAAAATTAAAAACGGTTGAATCAAAATATAAAGATGCAAAAAATGAATATTTTTGA", "aa": "MLQSEFAKLQYGVEIELGSKLDDLFSELNQFDLRNIDAKNLKFDTLFKKFKDIEILYRTHEMKLFAFVNATIAKYNKGLSFLKRKEYLDSKKEYEEVIKKIYKPFSKPNRFLNRVRFFSKLLETKKSSKSSIKKFVNKLRRDLNIENYYYKYELKKTIKQSSFIHFYKNLYFNGLLLKFINKNLKAILGLKEAKSFELLYEIFDLKNNITNEVNVISNIDNQKELKNEIKAVIKNNFLNDVMPYIERIKASSYEHTQEVINDVTREIQTSNNYDLNIGEFGLANKAFEKLKTVESKYKDAKNEYF", "aa_hexdigest": "a732cc87e6793edd582c75f6dff30eb1", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 55072, "stop": 56334, "observations": { "mutation": [ 55342, 56287 ], "stop": [ 55342, 56287 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E424311", "query_cov": 1.0, "subject_cov": 0.6475583864118896, "identity": 0.987, "score": 570.0, "evalue": 1.97e-202, "gene_start": 1, "gene_end": 305, "reference_start": 147, "reference_end": 451, "reference_sequence": "MKKIAISSLKVTNLKSINVVNEQFKNLINNLSFTLNANNILGFVGGDSETNNLIFNYLTNNTINPKRYQGYIEFQTNLGEYSSIYKNSNYQNNIAFGFYNDILNNDLNEDNSYRNIKKYINHGAVIGALTEEFVSQWKPVFQDYKLLLQSEFAKLQYGVEIELGSKLDDLFSELSQFDLRNIDAKNLKFDTLFKKFKDIEILYRTHEMKLFAFVNATIAKYNKGLSFLKRKEYLDSKKEYEEVIKKIYKPFSKPNRFLNRVRFFSKFLETKKSSKSSIKKFVNKLRRDLNIENYYYKYELKKTIKQSSFIHFYKNLYFNGLLLKFINKNLKAILGLKEAKSFELLYEIFDLKNNITNEVNVISNIDNQKELKNEIKAVIKNNFLNDVMPYIERIKASSYEHTQEVINDVTREIQTSNNYDLNIGEFGLANKAFEKLKIVESKYKDAKNEYFWNYKTEGNAILKETKSFKKF" }, "paralog": false, "identity": 0.9880952380952381, "score": 823.0, "evalue": 2.23e-304, "description": "Internal stop codon at 55342, 56287. Nonsense mutation around 55342, 56287.", "psc": { "uniref90_id": "UniRef90_UPI001E424311", "uniref50_id": "UniRef50_UPI001E424311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E424311", "UniRef:UniRef50_UPI001E424311" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E424311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E424311" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 36370.566700000054, "isoelectric_point": 9.476159477233889 }, "id": "DFOKDJOJIG_135", "locus": "GCA013367855_00095" }, { "type": "cds", "sequence": "contig_1", "start": 56383, "stop": 57069, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAACTAAAAATTTTTAATTTGATTAATTTAATAAAAATTAAGGAATTTAATAATTCAGTTCTAAATGAGCAAGTAAAAAAAATAAAAGAAAATCTACAATTTTTAAAAATTTTAAGAAATGAATTTGATTCTTATAAATATGTTGTAAGCGATTTTAAAACGGCATCAAAAAAACAAATAAAACTTTTTGTTTCAAAAATTAATATTTACAAAATTATTGCGAAGGCAAATATTGACGTTAATTTTTTAGTAGAGAAATTAGGCTCTTTAAGCGATGATGAAAAAATTAGATTAGAGTGCGAAAAAATTTTAATTAATAATCCTTCACTTATAATAATAGGACCCAATATTTCTAAATTACCATATGAAAAGCAATGCGAAACATTGAATCAATTGAATAAATTCGTTTTGGACAATCACAAAATGGCAATTTATTTCTTAGACAATATAAATATTGCTCAAAAAATAACTACAGATTTATATATTATAAATTCATCAAAAATTATTGAGCAAGGAAAAACTAGTCAATTAATAAGGAACCCAATAAATCCTATAGTCAAAAATATGTTAAATTATAAAGACGAGAAGACTCAAGAGGATTTAAGCTTTTATATGTCTCAAGAAGACAATATGGAAAACATATACAAATATGAAATAGAGCCATACCACTTTGTTTGA", "aa": "MKLKLKIFNLINLIKIKEFNNSVLNEQVKKIKENLQFLKILRNEFDSYKYVVSDFKTASKKQIKLFVSKINIYKIIAKANIDVNFLVEKLGSLSDDEKIRLECEKILINNPSLIIIGPNISKLPYEKQCETLNQLNKFVLDNHKMAIYFLDNINIAQKITTDLYIINSSKIIEQGKTSQLIRNPINPIVKNMLNYKDEKTQEDLSFYMSQEDNMENIYKYEIEPYHFV", "aa_hexdigest": "fc76707b2e7949d3adbb25551eb66642", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26868.107300000025, "isoelectric_point": 9.02049694061279 }, "id": "DFOKDJOJIG_136", "locus": "GCA013367855_00096" }, { "type": "cds", "sequence": "contig_1", "start": 57130, "stop": 57315, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTATTTATTCCGCAAAATACTTTTCAAAGTAAAGGAAATAAAACAATAGACAATAAGGAACCATTCGAAGAAACAACGCTTATAAATTTAATCGATGACGAAGATAACGAACTAAGGAATTTGAACATGGAAAAAACTTTTAATCATAAAATAGTTGAAAAAGACAGATGCAAAAAATGA", "aa": "MSLFIPQNTFQSKGNKTIDNKEPFEETTLINLIDDEDNELRNLNMEKTFNHKIVEKDRCKK", "aa_hexdigest": "d00d099dfced2fa91f162c6214778f5a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7257.111500000001, "isoelectric_point": 5.284571266174315 }, "id": "DFOKDJOJIG_137", "locus": "GCA013367855_00097" }, { "type": "cds", "sequence": "contig_1", "start": 57322, "stop": 57483, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGAAGATTTTTCAAACTCACGATGAATCTAAAAAACCATTCACCATTATTTTGCCACCCCCAAATGTAACCGGCAATCTCCATATTGGCCATGCCCTAGACAATTATATTTCTGATACAATAATAAGGTTTAAGAAGTTACAGGGATATGATGTATTGTGA", "aa": "MKIFQTHDESKKPFTIILPPPNVTGNLHIGHALDNYISDTIIRFKKLQGYDVL", "aa_hexdigest": "a7f9fee12811d4164b5043ad99892ddd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 47, "aa_cov": 0.8867924528301887, "hmm_cov": 1.0, "evalue": 1.620503749529741e-17, "score": 58.95497131347656, "start": 6, "stop": 52 } ], "seq_stats": { "molecular_weight": 6078.990000000001, "isoelectric_point": 8.123548316955567 }, "id": "DFOKDJOJIG_138", "locus": "GCA013367855_00098" }, { "type": "cds", "sequence": "contig_1", "start": 57655, "stop": 57780, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGCCCTTGATTATGGTTTTGAAAGATTCACTTTAGATGATAATTCTAATGAAGCCGTATTAAAGGTATTTATTGAACTTTATGAAAATGGTTTAATATATCGTGACAAAAAAGCAATAAATTGA", "aa": "MALDYGFERFTLDDNSNEAVLKVFIELYENGLIYRDKKAIN", "aa_hexdigest": "abc8b3314988d02db92af6055c58a28c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 39, "aa_cov": 0.9512195121951219, "hmm_cov": 1.0, "evalue": 1.8699996900636123e-09, "score": 32.398719787597656, "start": 3, "stop": 41 } ], "seq_stats": { "molecular_weight": 4829.396900000001, "isoelectric_point": 4.507070732116699 }, "id": "DFOKDJOJIG_139", "locus": "GCA013367855_00099" }, { "type": "cds", "sequence": "contig_1", "start": 57844, "stop": 58326, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "ATGTACTACATAAAATATCCAATTAAGAATTCAAAAAACTTTCTTGAAGTAGCCACAACGAGACCCGAAACAATGTTCTCAGATGTAGCTTTAGCAATAAATCCGCAAGATAGTAGAGCAAAACAATTAATAAAAGAAATAATAATACATCCATTAACAAATAAAGAAATTCCAATTATTTTATCAAACAAAATAGATCCTAATTTTGGTAGTGGAATAATGAAAGTTTCAGCCCATGCTGTTGATGATATTGAAATTATTATTGAAAATAAACTTCCAATAAATGAATGTATCAATAAAGATGGGAAATTAAATGATTTAGCAGGTGAATTTAAGGGATTAGATCGCTTTGAAGCTAGAGAAAAAATTGCACACTTATTAAGTCAAAAAGGACTTTTAATAAAAGAAGAAGAAGTAGTTTCTAATATTGGGTATAGTGAAAGATCAAAGGCCCCAATTGAAATTTTAGTTCAGCCACAATGA", "aa": "MYYIKYPIKNSKNFLEVATTRPETMFSDVALAINPQDSRAKQLIKEIIIHPLTNKEIPIILSNKIDPNFGSGIMKVSAHAVDDIEIIIENKLPINECINKDGKLNDLAGEFKGLDRFEAREKIAHLLSQKGLLIKEEEVVSNIGYSERSKAPIEILVQPQ", "aa_hexdigest": "72c9fd519ff81e9392659af94b28fce3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 60, "aa_cov": 0.36875, "hmm_cov": 1.0, "evalue": 3.328885491561792e-11, "score": 38.63521957397461, "start": 47, "stop": 105 } ], "seq_stats": { "molecular_weight": 18039.706699999984, "isoelectric_point": 5.959647941589356 }, "id": "DFOKDJOJIG_140", "locus": "GCA013367855_00100" }, { "type": "cds", "sequence": "contig_1", "start": 58708, "stop": 58962, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTATTTCTTTAGTTTATATTTTATGGATCAAAAACCTTTTGAACATCTTTTATTGCATGGATTAGTTAGAGATTCGCAAGGAAGAAAAATGTCTAAATCGCTTGGTAATGGAATTAATCCAATTCAAGTAATTGATGAATACGGTTCTGATGTTTTAAGAATGTCTTTAATTTTTAATTGCACACCTGGCCTTGATATTAATTACAGTGATGAAAAAATCCAAGGCGCTAAACTATTTGCTAATAAATTCTGA", "aa": "MYFFSLYFMDQKPFEHLLLHGLVRDSQGRKMSKSLGNGINPIQVIDEYGSDVLRMSLIFNCTPGLDINYSDEKIQGAKLFANKF", "aa_hexdigest": "2c3b7d7103de409c769653338b70449a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001D01CCB6", "query_cov": 0.9642857142857143, "subject_cov": 0.9310344827586207, "identity": 0.58, "score": 106.0, "evalue": 1.57e-28, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PEY5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 69, "aa_cov": 0.8214285714285714, "hmm_cov": 0.9855072463768116, "evalue": 3.4799059571578043e-22, "score": 74.33128356933594, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 9653.03739999999, "isoelectric_point": 6.705830192565918 }, "id": "DFOKDJOJIG_141", "locus": "GCA013367855_00101" }, { "type": "cds", "sequence": "contig_1", "start": 59248, "stop": 59502, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGTTTCTAACAGATTATGTTTTTGAAAAATTATATCATGAAGAATTGCTATGCAGCAATCTTCAAACTTTTGCTTTAAATAAAGAAAGTTCAAACAATACTCCTAAAATTATTTCTTTAATTACTGCTTTAAGAAAATACAGGGAAGATAAAAAGATTTCAAAATCTATTGAATTATTCTTCTATTTGAATAATCTTGTATTCAGCAGTATAGATTTAAAAATAATTAACAAATTAAGCAATTTTTCTTGA", "aa": "MPFLTDYVFEKLYHEELLCSNLQTFALNKESSNNTPKIISLITALRKYREDKKISKSIELFFYLNNLVFSSIDLKIINKLSNFS", "aa_hexdigest": "375f586a6051fff53124d3f451dfcd1f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9885.36759999999, "isoelectric_point": 8.733677101135253 }, "id": "DFOKDJOJIG_142", "locus": "GCA013367855_00102" }, { "type": "cds", "sequence": "contig_1", "start": 59665, "stop": 59781, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAGGCTAATAAAAATACAATCAATGAAAAATTAGCAAATTTAAAATTGCATAAAGAAAAATTAGAGTTTTATAAAAACCAACTCAAAGAAAAACAAGAAAAAATAAATTAA", "aa": "MEKANKNTINEKLANLKLHKEKLEFYKNQLKEKQEKIN", "aa_hexdigest": "8e4876d05b6b34e5405b5ce13d428f5c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4629.3415, "isoelectric_point": 9.574602699279783 }, "id": "DFOKDJOJIG_143", "locus": "GCA013367855_00103" }, { "type": "cds", "sequence": "contig_1", "start": 60101, "stop": 60625, "strand": "-", "frame": 3, "gene": "pgk", "product": "Phosphoglycerate kinase", "db_xrefs": [ "COG:COG0126", "COG:G", "SO:0001217", "UniRef:UniRef50_P62415", "UniRef:UniRef90_D1J7I6" ], "nt": "GTGTATGTTAATGATGCTTTTGGAACTGCACACAGAGCTCATGCATCAACATATGGAATTTCACAATATTCTAAAGAATCAGCATTAGGATTTTTAATGAATAATGAAGTTGAACACCTTCAAAAATTATTAGTAGGATTTAAACGTCCTTTTGTTGCAATAATTGGTGGAGCAAAAGTCAGCGATAAAATCAAAGTTCTAGAAAAGTTATTTGAACTAGCAGATAAGGTTTTAATTGTTGGTGGTATGGCATATACATTCCAAAAAGCCTTAAATAGAGAAATTGGAACTTCTATTTTTGAAGAAGATAAAGTTGAAATGGCAAAAGAATATTTAAACAAATATAAAGATAAAATTGTTTTGCCAATTGACAATGCTTATGTCGATGAATTTGCTGACAAAACTCCTAAATTTACAAGTCCAGAAAATCCCGATATTCCTGATGGAAATATGGGATTAGATATTGGACCAGAAAGTATTAAATTATTTGCAAGTATTATTAAAAATGCTAAAACTATCTTTTGA", "aa": "MYVNDAFGTAHRAHASTYGISQYSKESALGFLMNNEVEHLQKLLVGFKRPFVAIIGGAKVSDKIKVLEKLFELADKVLIVGGMAYTFQKALNREIGTSIFEEDKVEMAKEYLNKYKDKIVLPIDNAYVDEFADKTPKFTSPENPDIPDGNMGLDIGPESIKLFASIIKNAKTIF", "aa_hexdigest": "47dab2524869662c40012219827175a2", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "genes": [ "pgk" ], "truncated": "both", "pseudogene": { "start": 59861, "stop": 60925, "observations": { "mutation": [ 60103, 60643 ], "stop": [ 60103, 60643 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I6", "query_cov": 1.0, "subject_cov": 0.43828715365239296, "identity": 0.9890000000000001, "score": 340.0, "evalue": 8.52e-115, "gene_start": 1, "gene_end": 174, "reference_start": 144, "reference_end": 317, "reference_sequence": "MKKTIEDLQLENKKVILRVDFNVPISNNTIMDTKRIDEELPTIKYILSKNASLIILSHLGRVKTEEDKKTKSLEIVAKKLAQLLNRDVKFINENRSEAVTNAAKNLKPGEILVLENTRFQDLNNKAESNNDEDLAKYWASLGDVYVNDAFGTAHRAHASTYGISQYSKESALGFLMNNEVEHLQKLLVGFKRPFVAIIGGAKVSDKIKVLEKLFELADKVLIVGGMAYTFQKALNRKIGTSIFEEDKVEMAKEYLNKYKDKIVLPIDNAYVDEFADKTPKFTSPENPDIPDGNMGLDIGPESIKLFASIIKNAKTIFWNGPAGVTEFSNFEAGTKGIATAIAQNRDCYSVVGGGDSVTAINKLGYQDKFSFISTGGGASIEFVQGKKLPGIEAIQDK" }, "paralog": false, "identity": 0.9915254237288136, "score": 683.0, "evalue": 1.06e-252, "description": "Internal stop codon at 60103, 60643. Nonsense mutation around 60103, 60643.", "psc": { "uniref90_id": "UniRef90_D1J7I6", "gene": "Pgk", "product": "Phosphoglycerate kinase", "uniref50_id": "UniRef50_P62415", "cog_id": "COG0126", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I6", "UniRef:UniRef50_P62415", "COG:COG0126", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_P62415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P62415" ], "product": "Phosphoglycerate kinase" } }, "id": "DFOKDJOJIG_144", "locus": "GCA013367855_00104" }, { "type": "cds", "sequence": "contig_1", "start": 60641, "stop": 61054, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGAAGAAAACCATTGAAGACCTTCAATTAGAAAACAAAAAGGTTATTTTAAGAGTTGATTTTAACGTTCCTATTTCAAACAATACTATTATGGACACAAAAAGAATTGATGAAGAATTGCCAACAATCAAATACATATTGAGTAAAAATGCTTCTTTAATCATTCTATCTCATTTAGGAAGAGTTAAAACAGAAGAAGACAAGAAAACTAAATCTTTAGAAATTGTAGCTAAAAAATTGGCGCAATTATTAAATAGAGACGTTAAATTTATTAACGAAAATAGAAGCGAAGCAGTTACAAATGCTGCTAAAAACTTAAAACCTGGAGAAATTTTGGTTTTAGAAAATACTCGTTTCCAAGATTTAAATAATAAAGCAGAGTCTAATAATGATGAAGATCTTGCAAAATATTGA", "aa": "MKKTIEDLQLENKKVILRVDFNVPISNNTIMDTKRIDEELPTIKYILSKNASLIILSHLGRVKTEEDKKTKSLEIVAKKLAQLLNRDVKFINENRSEAVTNAAKNLKPGEILVLENTRFQDLNNKAESNNDEDLAKY", "aa_hexdigest": "515c239c6121c3015d4e53237a087b3c", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 133, "aa_cov": 0.9635036496350365, "hmm_cov": 0.9548872180451128, "evalue": 2.31560865172479e-40, "score": 134.35263061523438, "start": 5, "stop": 136 } ], "seq_stats": { "molecular_weight": 15706.88729999998, "isoelectric_point": 8.729293251037596 }, "id": "DFOKDJOJIG_145", "locus": "GCA013367855_00105" }, { "type": "cds", "sequence": "contig_1", "start": 61166, "stop": 61342, "strand": "+", "frame": 2, "gene": null, "product": "YARHG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYF4", "UniRef:UniRef90_D1J7I7" ], "nt": "ATGGCAAAAATTTTAAATGCTCAAAAGCAAAAGGACACAAGAACTCTTAGCTATGATCCTGAAAAAGACAGCTTGACTTTGCTAATTTCAGAGTTTCAACTTTACAATAAAAACACCAAATTTATTAATGATTATTCAGAAATGGAAAAATTTCATTATTTTAAAGAATCTAGATGA", "aa": "MAKILNAQKQKDTRTLSYDPEKDSLTLLISEFQLYNKNTKFINDYSEMEKFHYFKESR", "aa_hexdigest": "c5016de7e57661d77b4a08d5e9f9db54", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 61166, "stop": 61573, "observations": { "mutation": [ 61340, 61490 ], "stop": [ 61340, 61490 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I7", "query_cov": 1.0, "subject_cov": 0.4264705882352941, "identity": 1.0, "score": 114.0, "evalue": 1.98e-31, "gene_start": 1, "gene_end": 58, "reference_start": 1, "reference_end": 58, "reference_sequence": "MAKILNAQKQKDTRTLSYDPEKDSLTLLISEFQLYNKNTKFINDYSEMEKFHYFKESRWMKTMDEKLQDQVQEFSKRQKKLNAKYKSYIEYSKWSESPYATKTKPLTKWNRIIIISVFIILVIIMLLIVIGLNKLW" }, "paralog": false, "identity": 0.9851851851851852, "score": 245.0, "evalue": 1.76e-85, "description": "Internal stop codon at 61340, 61490. Nonsense mutation around 61340, 61490.", "psc": { "uniref90_id": "UniRef90_D1J7I7", "uniref50_id": "UniRef50_A0A448ZYF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I7", "UniRef:UniRef50_A0A448ZYF4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYF4" ], "product": "YARHG domain-containing protein" } }, "id": "DFOKDJOJIG_146", "locus": "GCA013367855_00106" }, { "type": "cds", "sequence": "contig_1", "start": 61343, "stop": 61492, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F36E0B6", "UniRef:UniRef90_UPI001F36E0B6" ], "nt": "ATGAAAACAATGGATGAAAAATTACAAGATCAAGTGCAAGAATTTTCAAAAAGGCAAAAAAAATTAAATGCCAAATATAAAAGTTACATTGAATATTCTAAATGGTCTGAATCGCCATATGCAACAAAAACTAAACCGCTTACAAAATGA", "aa": "MKTMDEKLQDQVQEFSKRQKKLNAKYKSYIEYSKWSESPYATKTKPLTK", "aa_hexdigest": "78f0925452087919a2505a5dae3b3c70", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 61307, "stop": 61573, "observations": { "mutation": [ 61340, 61490 ], "stop": [ 61340, 61490 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F36E0B6", "query_cov": 1.0, "subject_cov": 0.5568181818181818, "identity": 0.9390000000000001, "score": 85.1, "evalue": 1.29e-20, "gene_start": 1, "gene_end": 49, "reference_start": 13, "reference_end": 60, "reference_sequence": "MEKFHYFKESRWMKTMDEKLQDQVQEFSKGKKLNAKYKSYIEYSKWSESPYAAKTKPLTKWNRIIIISVFIILVIIMLLIVIGLNKLW" }, "paralog": false, "identity": 0.9431818181818182, "score": 142.0, "evalue": 8.05e-46, "description": "Internal stop codon at 61340, 61490. Nonsense mutation around 61340, 61490.", "psc": { "uniref90_id": "UniRef90_UPI001F36E0B6", "uniref50_id": "UniRef50_UPI001F36E0B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F36E0B6", "UniRef:UniRef50_UPI001F36E0B6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F36E0B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F36E0B6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5933.764700000001, "isoelectric_point": 9.698575401306151 }, "id": "DFOKDJOJIG_147", "locus": "GCA013367855_00107" }, { "type": "cds", "sequence": "contig_1", "start": 61716, "stop": 62174, "strand": "+", "frame": 3, "gene": null, "product": "DAC domain-containing protein", "db_xrefs": [ "EC:2.7.7.85", "KEGG:K18672", "SO:0001217", "UniParc:UPI0001BC204C", "UniRef:UniRef100_D1J7I8", "UniRef:UniRef50_D1J7I8", "UniRef:UniRef90_D1J7I8" ], "nt": "TTGGGTGAATCTACAAAAATAAGAATTATTTATCAATTAAAAGAAGCCCTTGAATTTTTATCAAAGACAAAGACTGGTGCAATTATTACTATTGAAAACAAAGATAATCTTGATTTGCTAAGAACTGATGGTGTAATATTAAATGCTAACATTTCTGCTTCTTTAATTATTTCTATTTTTAATAAACACGCTCCACTTCACGATGGCGCAATAATTATTAGAAATAACAAAATTTATTATGCTGCCACATATTATAAAATAACAAAAAAATCAATTGATAATAAATATGGTGCAAGACACCGTGCTGCTATGGGTATTTCAGAAATTTGCGATGCTACAACTATAGTTGTTAGTGAAGAAAATGGCGGAATTACAATAGCTAAAAACGGAACTTTTAGCCCTGTTTTACTAGAAAATCTACAAGAAAGATTAGTCAATATTTTTAAGGATAATAATTAA", "aa": "MGESTKIRIIYQLKEALEFLSKTKTGAIITIENKDNLDLLRTDGVILNANISASLIISIFNKHAPLHDGAIIIRNNKIYYAATYYKITKKSIDNKYGARHRAAMGISEICDATTIVVSEENGGITIAKNGTFSPVLLENLQERLVNIFKDNN", "aa_hexdigest": "0118841dcdf0adc1110f00f51ad17697", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC204C", "uniref100_id": "UniRef100_D1J7I8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC204C", "UniRef:UniRef100_D1J7I8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7I8", "uniref90_id": "UniRef90_D1J7I8" }, "psc": { "uniref90_id": "UniRef90_D1J7I8", "product": "DAC domain-containing protein", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_D1J7I8", "kegg_orthology_id": "K18672" }, "pscc": { "uniref50_id": "UniRef50_D1J7I8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I8" ], "product": "DAC domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_148", "locus": "GCA013367855_00108" }, { "type": "cds", "sequence": "contig_1", "start": 62748, "stop": 63941, "strand": "+", "frame": 3, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "RefSeq:WP_175972176.1", "SO:0001217", "UniParc:UPI00158D76A7", "UniRef:UniRef100_UPI00158D76A7", "UniRef:UniRef50_O83217", "UniRef:UniRef90_B3PMU1" ], "nt": "ATGGCAAAATTAGATTTTGACCGTTCAAAACCACACGTTAATATTGGTACTATCGGTCACGTTGACCACGGTAAAACTACATTAACTGCTGCTATTGCAACAGTATTAGCTAAAAAAGGTCTAGCAGAAGCTAGAGACTATGCTTCTATTGACAATGCACCAGAAGAAAAAGCACGTGGTATTACAATTAACACATCACACATTGAATATCAAACAGAAAAACGTCACTACGCACACGTTGACTGTCCAGGACACGCTGACTATGTTAAAAACATGATTACCGGTGCTGCCCAAATGGATGGAGCAATTTTAGTTGTTGCTGCTACTGATGGTCCTATGCCTCAAACTCGTGAACACATTTTACTTGCAAGACAAGTTGGTGTTCCAAAGATAGTTGTATTTTTAAACAAAATTGATATGTTTAAAGATGATGAAAGAGAAGAAATGGTTGGACTAGTAGAAATGGACGTAAGAAGCCTTCTATCAGAATATGGTTTTGATGGCGACAATGCTCCTATTATTGCTGGTTCAGCTCTTAAAGCATTACAAGGAGATCCAGAATACGAAAAAGGTATTTTGGAATTAATGGATGCTGTTGATACATACATCGAAGAACCAAAACGTGAAACAGACAAACCATTCTTAATGGCTGTAGAAGACGTATTTACAATCACTGGTCGTGGAACTGTTGCAACTGGTAGAGTTGAACGTGGTGTTTTACAATTAAACGAAGAAGTTGAAATTGTTGGATTGAAACCAACCAAGAAAACAGTTGTTACTGGTATTGAAATGTTCCGTAAGAACTTAAAAGAAGCACAAGCTGGAGATAACGCTGGATTATTGCTACGTGGAATTGACAGAAGCGAAGTTGAACGTGGACAAGTTTTAGCAAAACCTAAGACAATCGTTCCTCACATACAATTCGAAGCAACTGTTTATGTTCTTAAAAAAGAAGAAGGTGGACGTCACACACCATTCTTCCACAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACTGGTGGAATTGAATTCAAACCTGGACGTGAAATGGTTGTTCCTGGCGACAACGTAGAATTAACTGTTACTTTAATTGCTCCTATTGCTATTGAAGAAGGAACCAAGTTCTCTATTCGTGAAGGTGGAAGAACTGTTGGTGCCGGATCAGTTACAAAAATTCTTAAATAA", "aa": "MAKLDFDRSKPHVNIGTIGHVDHGKTTLTAAIATVLAKKGLAEARDYASIDNAPEEKARGITINTSHIEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVPKIVVFLNKIDMFKDDEREEMVGLVEMDVRSLLSEYGFDGDNAPIIAGSALKALQGDPEYEKGILELMDAVDTYIEEPKRETDKPFLMAVEDVFTITGRGTVATGRVERGVLQLNEEVEIVGLKPTKKTVVTGIEMFRKNLKEAQAGDNAGLLLRGIDRSEVERGQVLAKPKTIVPHIQFEATVYVLKKEEGGRHTPFFHNYKPQFYFRTTDVTGGIEFKPGREMVVPGDNVELTVTLIAPIAIEEGTKFSIREGGRTVGAGSVTKILK", "aa_hexdigest": "4dd75f08e4f93aa808344aed1ff4c63d", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158D76A7", "ncbi_nrp_id": "WP_175972176.1", "uniref100_id": "UniRef100_UPI00158D76A7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D76A7", "RefSeq:WP_175972176.1", "UniRef:UniRef100_UPI00158D76A7" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D76A7", "uniref90_id": "UniRef90_B3PMU1" }, "psc": { "uniref90_id": "UniRef90_B3PMU1", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_O83217", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_O83217", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O83217" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "DFOKDJOJIG_149", "locus": "GCA013367855_00109" }, { "type": "cds", "sequence": "contig_1", "start": 64215, "stop": 65918, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175972177.1", "SO:0001217", "UniParc:UPI00158EBA0F", "UniRef:UniRef100_UPI00158EBA0F", "UniRef:UniRef50_A0A6A8Q130", "UniRef:UniRef90_UPI00158EBA0F", "PFAM:PF04778.17" ], "nt": "ATGAATGAAAAAAAGAAAAAAATTGCCATTCCATTGGCAATTTTGTGTGGCGGTTTAGCAATTGCTACAACAGCGCTTATTGCAATAAAGGCTCGCCGTCATAAAATTGCAAATCAATCGCAAAAAGAAAATTTATTGCAAAATTTTAAAAAATTACAAAAGCAATTAAATGAATTGCTAGGATATAAAATTGTTAATGAAATAAATGTTTTTCATGAACAAGAAGTTTTGCAAGGATCACTAAAAATTAATAATAAATCTGAAACCAAAGTAATTGAAGAAGAAACTTTAAGGTTAAAAGATGCTATTACTTTATTAATATCAAAAATAAAAAATCAAATAAATCAAAAAGAATTGGAATTTGCTAAATTTAATGAAATAAAAGATAAATTGCAGGAATATATCAAAAACGAGTTATCGAAACAAGAATACGAACATATTAAACAAAATATCGAAAATGAATTAAATAAATATACTCCAATTAGTCTCGAATCAACTTTAATCGAAATACAAAATGCAACAAATAATCTAATTAAATTATTAAATGAATCAACCAAAGAAAAAGATAACATTGATAATTTAAATGCCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAATTAAAAATGCAAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAATAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAG", "aa": "MNEKKKKIAIPLAILCGGLAIATTALIAIKARRHKIANQSQKENLLQNFKKLQKQLNELLGYKIVNEINVFHEQEVLQGSLKINNKSETKVIEEETLRLKDAITLLISKIKNQINQKELEFAKFNEIKDKLQEYIKNELSKQEYEHIKQNIENELNKYTPISLESTLIEIQNATNNLIKLLNESTKEKDNIDNLNAKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEIKNANQAVASNNTASMQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLIAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKANQAVASNNTASMQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKLIKQLLQTILLQCNQLRLH", "aa_hexdigest": "a84f2288762d0a852d1b4adaaa69cb27", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158EBA0F", "ncbi_nrp_id": "WP_175972177.1", "uniref100_id": "UniRef100_UPI00158EBA0F", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EBA0F", "RefSeq:WP_175972177.1", "UniRef:UniRef100_UPI00158EBA0F" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158EBA0F", "uniref90_id": "UniRef90_UPI00158EBA0F" }, "psc": { "uniref90_id": "UniRef90_UPI00158EBA0F", "uniref50_id": "UniRef50_A0A6A8Q130" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q130", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q130" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 157, "aa_cov": 0.2751322751322751, "hmm_cov": 1.0, "evalue": 2.291550503106247e-124, "score": 406.7081298828125, "start": 363, "stop": 518 } ], "seq_stats": { "molecular_weight": 63260.74370000023, "isoelectric_point": 9.241365623474117 }, "id": "DFOKDJOJIG_150", "locus": "GCA013367855_00110" }, { "type": "cds", "sequence": "contig_1", "start": 65894, "stop": 67330, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q130", "UniRef:UniRef90_UPI001F524FF2", "PFAM:PF04778.17" ], "nt": "ATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAATAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAAGCTAATCAAGCAGTTGCTTTAAACAATACTGCTTCAATGCAATCAGCTAAGACTACATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAG", "aa": "MQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKANQAVASNNTASMQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLIAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKANQAVALNNTASMQSAKTTLDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKLIKQLLQTILLQCNQLRLH", "aa_hexdigest": "77a724c7d619444aa229cffd8b8906a8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F524FF2", "query_cov": 1.0, "subject_cov": 0.9617706237424547, "identity": 0.9520000000000001, "score": 728.0, "evalue": 3.99e-261, "valid": true, "uniref50_id": "UniRef50_A0A6A8Q130" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q130", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q130" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 157, "aa_cov": 0.3263598326359833, "hmm_cov": 1.0, "evalue": 2.1901961388323436e-162, "score": 530.254150390625, "start": 274, "stop": 429 } ], "seq_stats": { "molecular_weight": 52541.93860000006, "isoelectric_point": 9.401440620422363 }, "id": "DFOKDJOJIG_151", "locus": "GCA013367855_00111" }, { "type": "cds", "sequence": "contig_1", "start": 67306, "stop": 67800, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158B95E8", "UniRef:UniRef50_UPI001F51C84F", "UniRef:UniRef90_UPI001F51C84F", "PFAM:PF04778.17" ], "nt": "ATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAG", "aa": "MQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKLIKQLLQTILLQCNQLRLH", "aa_hexdigest": "29ced4a8a24ab5db9a904283eaeb858c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00158B95E8", "query_cov": 1.0, "subject_cov": 0.8961748633879781, "identity": 0.86, "score": 223.0, "evalue": 3.95e-72, "valid": false, "uniref50_id": "UniRef50_UPI00158B95E8" }, "pscc": { "uniref50_id": "UniRef50_UPI00158B95E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158B95E8" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 67007, "stop": 67800, "observations": { "deletion": [ 67268 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F51C84F", "query_cov": 1.0, "subject_cov": 0.4823529411764706, "identity": 0.9570000000000001, "score": 262.0, "evalue": 3.99e-85, "gene_start": 1, "gene_end": 164, "reference_start": 177, "reference_end": 340, "reference_sequence": "MSPRNSKANQAVASNNTASMQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKANQAVASNNTASMQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKLIKQLLQTILLQCNQLRLH" }, "paralog": false, "identity": 0.9393939393939394, "score": 428.0, "evalue": 4.98e-153, "description": "Frameshift due to deletion around 67268.", "psc": { "uniref90_id": "UniRef90_UPI001F51C84F", "uniref50_id": "UniRef50_UPI001F51C84F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F51C84F", "UniRef:UniRef50_UPI001F51C84F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F51C84F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51C84F" ] } }, "hypothetical": true, "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 116, "aa_cov": 0.7012195121951219, "hmm_cov": 1.0, "evalue": 1.6681188217266886e-49, "score": 163.44142150878906, "start": 1, "stop": 115 } ], "seq_stats": { "molecular_weight": 18264.36559999999, "isoelectric_point": 9.408209800720215 }, "id": "DFOKDJOJIG_152", "locus": "GCA013367855_00112" }, { "type": "cds", "sequence": "contig_1", "start": 67776, "stop": 68741, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q130", "UniRef:UniRef50_UPI001F51C84F", "UniRef:UniRef90_UPI001F51C84F", "UniRef:UniRef90_UPI001F524FF2", "PFAM:PF04778.17" ], "nt": "ATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTACATTAG", "aa": "MQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKANQAVASNNTASMQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKLIKQLLQTILLQCNQLRLH", "aa_hexdigest": "8aff2001f690f73fb075f22ad15dad5f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F51C84F", "query_cov": 1.0, "subject_cov": 0.9441176470588235, "identity": 0.9279999999999999, "score": 466.0, "evalue": 5.96e-163, "valid": true, "uniref50_id": "UniRef50_UPI001F51C84F" }, "pscc": { "uniref50_id": "UniRef50_UPI001F51C84F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51C84F" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 67719, "stop": 69043, "observations": { "deletion": [ 68679 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F524FF2", "query_cov": 1.0, "subject_cov": 0.6458752515090543, "identity": 0.953, "score": 503.0, "evalue": 5.23e-175, "gene_start": 1, "gene_end": 321, "reference_start": 177, "reference_end": 497, "reference_sequence": "MSPRNSKANQAVASNNTASMQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKANQAVASNNTASMQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLIAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKANQAVALNNTASMQSAKTTLDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKLIKQLLQTILLQCNQLRLH" }, "paralog": false, "identity": 0.9886621315192744, "score": 736.0, "evalue": 1.94e-269, "description": "Frameshift due to deletion around 68679.", "psc": { "uniref90_id": "UniRef90_UPI001F524FF2", "uniref50_id": "UniRef50_A0A6A8Q130", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F524FF2", "UniRef:UniRef50_A0A6A8Q130" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q130", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q130" ] } }, "hypothetical": true, "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 157, "aa_cov": 0.48598130841121495, "hmm_cov": 1.0, "evalue": 9.951769644520695e-107, "score": 349.3936462402344, "start": 117, "stop": 272 } ], "seq_stats": { "molecular_weight": 35369.0721, "isoelectric_point": 9.403245735168458 }, "id": "DFOKDJOJIG_153", "locus": "GCA013367855_00113" }, { "type": "cds", "sequence": "contig_1", "start": 68717, "stop": 69571, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q130", "UniRef:UniRef50_UPI001F51C84F", "UniRef:UniRef90_UPI001F524FF2", "PFAM:PF04778.17" ], "nt": "ATGCAATCAGCTAAGACTACATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTACATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCGAACAAATCAGACATTATTGCTGCAAATGAAGCATTACAACAAGCATTAAATACTGCAAAGGCTAAAAAATCAAGTATAGATAATGAATTAAAACCTCTAAAGGATGATTTGCAAAGTAAAATTGAAGAGTTTGGACCTATAAGAAATACCAATTTTTCATGA", "aa": "MQSAKTTLDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKANQAVASNNTASMQSAKTTLDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALQQALNTAKAKKSSIDNELKPLKDDLQSKIEEFGPIRNTNFS", "aa_hexdigest": "52134e4fa457c874790f087be873a242", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F51C84F", "query_cov": 0.9577464788732394, "subject_cov": 0.8, "identity": 0.8240000000000001, "score": 352.0, "evalue": 9.77e-119, "valid": false, "uniref50_id": "UniRef50_UPI001F51C84F" }, "pscc": { "uniref50_id": "UniRef50_UPI001F51C84F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51C84F" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 68418, "stop": 69727, "observations": { "deletion": [ 68679 ], "stop": [ 69570 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F524FF2", "query_cov": 0.9577464788732394, "subject_cov": 0.5472837022132797, "identity": 0.912, "score": 408.0, "evalue": 2.52e-138, "gene_start": 1, "gene_end": 272, "reference_start": 177, "reference_end": 448, "reference_sequence": "MSPRNSKANQAVASNNTASMQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKANQAVASNNTASMQSAKTALDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLIAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKANQAVALNNTASMQSAKTTLDAKIAQVNQQLQQFNKEKENKFNELKQTRNQIDEFIKTNKSNSNYSALISKLTAAKEAKNSVVESSNKSDIIAANEALKQALQEANASKSQADNSNKLIKEQLNASITSANQLSAKLTDNDNNIQQAKAQLAQEIQKLIKQLLQTILLQCNQLRLH" }, "paralog": false, "identity": 0.8325688073394495, "score": 642.0, "evalue": 4.17e-233, "description": "Internal stop codon at 69570. Frameshift due to deletion around 68679.", "psc": { "uniref90_id": "UniRef90_UPI001F524FF2", "uniref50_id": "UniRef50_A0A6A8Q130", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F524FF2", "UniRef:UniRef50_A0A6A8Q130" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q130", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q130" ] } }, "hypothetical": true, "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 158, "aa_cov": 0.5528169014084507, "hmm_cov": 1.0, "evalue": 2.3866024194720184e-101, "score": 331.91156005859375, "start": 117, "stop": 273 } ], "seq_stats": { "molecular_weight": 31367.497400000004, "isoelectric_point": 9.339808845520018 }, "id": "DFOKDJOJIG_154", "locus": "GCA013367855_00114" }, { "type": "cds", "sequence": "contig_1", "start": 69713, "stop": 69841, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGACTATAACCGAAGAATTAGGAAAGGCTGAAACTAAAAATAGTAACATTGGATATAGATTGTTTAAATTGGCACAAGCTGAACAATTTAATAACACTGATGTTGATAAATTAAAGAACGCTTGA", "aa": "MKTITEELGKAETKNSNIGYRLFKLAQAEQFNNTDVDKLKNA", "aa_hexdigest": "1040c51cc4300251eb118171eedbc1a5", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4773.337500000002, "isoelectric_point": 8.064946556091307 }, "id": "DFOKDJOJIG_155", "locus": "GCA013367855_00115" }, { "type": "cds", "sequence": "contig_1", "start": 69926, "stop": 70087, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCACTCAAGAATCTACAATTAAAAAAGTTATAGTAAATATTCAAACACATATAAGAAATAACCTTAACTCACAATATAGATTAGAAGCTGACAAGCTTATTGCCAATATGAAGAGAGGTTATGGAGATAAAGTTGGTATAGAAAGCTTACAAAAATGA", "aa": "MSTQESTIKKVIVNIQTHIRNNLNSQYRLEADKLIANMKRGYGDKVGIESLQK", "aa_hexdigest": "6025493b7baad4b91443d4d811fa1f21", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6075.930100000001, "isoelectric_point": 9.77445468902588 }, "id": "DFOKDJOJIG_156", "locus": "GCA013367855_00116" }, { "type": "cds", "sequence": "contig_1", "start": 70097, "stop": 70204, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGATAGTGTGTTGAGTGTAGATGATAGTTTGAAAGACGACTTTAACAAAGCACTTAGAGTATTAGTTGGAGATTACACTAAAAACCCTCCTGTTTCATCGTGA", "aa": "MDDSVLSVDDSLKDDFNKALRVLVGDYTKNPPVSS", "aa_hexdigest": "7be23c76076d7d004b63c86430105fb0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3841.2133000000013, "isoelectric_point": 4.219522285461426 }, "id": "DFOKDJOJIG_157", "locus": "GCA013367855_00117" }, { "type": "cds", "sequence": "contig_1", "start": 70814, "stop": 71047, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A4F674", "UniRef:UniRef90_UPI0034A4F674" ], "nt": "TTGTCAACAGTTCCTGAATTTATTAAAGTTGCTCAAACAACAAGAAGCAATAACTTGCGATCACTGTCAGAACAATTTAAATTTGTATTGTTAGACATTAATAAGTATTTAAAAGAAGTAAAGAAAATACTAGACGAAAATAAAACATTAAATAAGGATAACATAACTAAATTAAACGAAAAAATAAGATTGTTTGTGGACAAATCCCGTGAATTAAAAGAATCATTAAGATGA", "aa": "MSTVPEFIKVAQTTRSNNLRSLSEQFKFVLLDINKYLKEVKKILDENKTLNKDNITKLNEKIRLFVDKSRELKESLR", "aa_hexdigest": "88c7e08c5ea83e0684ad7d1fa188749c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 70835, "stop": 71131, "observations": { "mutation": [ 71045 ], "stop": [ 71045 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A4F674", "query_cov": 0.9090909090909091, "subject_cov": 0.7142857142857143, "identity": 0.8140000000000001, "score": 103.0, "evalue": 1.89e-27, "gene_start": 8, "gene_end": 77, "reference_start": 1, "reference_end": 70, "reference_sequence": "MKVAQTTRSNNLRSLSEQFRITLLNIDKDLKEVKKILDENKTLDENNITKLNEKIKLFVDRSCELKESLRWFDNFHENISDQRIKNKLDEVRELLKNV" }, "paralog": false, "identity": 0.8163265306122449, "score": 151.0, "evalue": 6.11e-49, "description": "Internal stop codon at 71045. Nonsense mutation around 71045.", "psc": { "uniref90_id": "UniRef90_UPI0034A4F674", "uniref50_id": "UniRef50_UPI0034A4F674", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A4F674", "UniRef:UniRef50_UPI0034A4F674" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A4F674", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A4F674" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9141.552999999994, "isoelectric_point": 9.791796684265137 }, "id": "DFOKDJOJIG_158", "locus": "GCA013367855_00118" }, { "type": "cds", "sequence": "contig_1", "start": 71344, "stop": 72519, "strand": "+", "frame": 1, "gene": null, "product": "Lmp protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410EEB1", "UniRef:UniRef90_UPI00093C2025" ], "nt": "TTGACCTTTATTGCTGGGGGAGTTGCCATTACTGCTTTTGTTTTAATAATGTGTAGATTTTGCAAGAAACCTAATAAGCAAATAAAAGATCGAACCTCTTTAAATTTATTTAATAATGCGCAAAGTGAATTGAAAGAATTAATTAATTCTCCAATAGCCAAGCTATGTCAAACCGCTATTGAACAAAAAGTTTTAAACGATTCAAATAAAATAAATGAAAAGTCTACTGACGAAGAAATTTCAAAGAATACAGAATATTTAAATAGTTCAATCGCTTTGCTTAAAAATAAGTTAGATCTTGCTAAACAAGAATTAGAAAAATTCAACATGGCTATGCTTCAACTAAGATCATTAGTTAATAATAAAGATAGTAAAAGTATAGATACCAAAAATTCAATCAACGCTTTAAATAATGCTAAAAAATTGCCTTTTAATTCTATTGAACAAGTTAAGAGCGAATCTTTAACTTTGAAAAATAATATTGATGAATTAAAGTTAAAGCTTAAAGAAGTTAAAAAGTCTAGACTTGCAGAAGCCAAGAACAAATTAAAAGCAATTATTGATTCGGAACCTTCTAAATATGTTGATAGTTCTAGAGAATTGCAAATATTTAATGATTCAAATGTTGAAGCAAAATCAACACTTGAAGATATTGATAAAAAGATTGATGCCGCAATAAGAGCAAACAATGATTTAAATGAAAAAATTCAAAATTATAAATCAGCAAGATTATCTGATCTTGAATCTTCAAGAAATGATTTGTCAAACTTTATTGCAAACGATTTAAAAGATCCTGTATATAAAGAAATTGCAGATAAAATTAATGAAAAGATTAAAGAAGTAAAGGGCGTTGGAAAAAATTCGACAATCAAAGAATTACAAGTAGCCATAAAAACTTTAGCAGATGCTAAAGAAAAAGCAATTAAAGAAAAAGCTTGGATAGATAGAGTTGGTATTAATAAAGTTAGAGAAATTCAAAGATCTAAAATATTGTTGGTTGATGCTGAATATATTGCAAGATTTTTAAGATCAGGAAATGGTGAACCATTTAGACAAAAGAATGAATTTAATGAATATTCCGGAACAGAATTTTTCAAATTAAAAAATGAGTTGGAAAGGGTTTCAAATCTTAAAGATTTAAATTCTCTTGACAGTGTTGATTATAAAGTAATATGA", "aa": "MTFIAGGVAITAFVLIMCRFCKKPNKQIKDRTSLNLFNNAQSELKELINSPIAKLCQTAIEQKVLNDSNKINEKSTDEEISKNTEYLNSSIALLKNKLDLAKQELEKFNMAMLQLRSLVNNKDSKSIDTKNSINALNNAKKLPFNSIEQVKSESLTLKNNIDELKLKLKEVKKSRLAEAKNKLKAIIDSEPSKYVDSSRELQIFNDSNVEAKSTLEDIDKKIDAAIRANNDLNEKIQNYKSARLSDLESSRNDLSNFIANDLKDPVYKEIADKINEKIKEVKGVGKNSTIKELQVAIKTLADAKEKAIKEKAWIDRVGINKVREIQRSKILLVDAEYIARFLRSGNGEPFRQKNEFNEYSGTEFFKLKNELERVSNLKDLNSLDSVDYKVI", "aa_hexdigest": "ff522b78a5f33d659f4e9f62a60c178a", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI00093C2025", "query_cov": 0.9846547314578005, "subject_cov": 0.9344660194174758, "identity": 0.943, "score": 653.0, "evalue": 1.36e-234, "valid": true, "product": "Lmp protein", "uniref50_id": "UniRef50_UPI002410EEB1" }, "pscc": { "uniref50_id": "UniRef50_UPI002410EEB1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410EEB1" ], "product": "Lmp protein" }, "genes": [], "id": "DFOKDJOJIG_159", "locus": "GCA013367855_00119" }, { "type": "cds", "sequence": "contig_1", "start": 72784, "stop": 73047, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAGCTTCTCACTGTTGACATTCGCGATAAATTTAGAGAAGTTTATATAACTGAAGAAGCAAAGAAAAATAATCTTGCATATCAAGTTGTTGATTTATTAAATAAATTAGAATCATTTGATTTGAACAATAGTAAATTAGATAAACAAGAAATTAAAGATTTTAAAGAAAAATTAAGTTCAAAAAATCTAGAAATTGAAAAATATTTAAGTAGCGAAGAAACCTTTAAAGATTATCGCGCATATAACCCTGATTTATGA", "aa": "MLKLLTVDIRDKFREVYITEEAKKNNLAYQVVDLLNKLESFDLNNSKLDKQEIKDFKEKLSSKNLEIEKYLSSEETFKDYRAYNPDL", "aa_hexdigest": "fa95024ed22ba70e61ac81065a029d6c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10411.712899999993, "isoelectric_point": 5.210794258117674 }, "id": "DFOKDJOJIG_160", "locus": "GCA013367855_00120" }, { "type": "cds", "sequence": "contig_1", "start": 73048, "stop": 73206, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002735871D", "UniRef:UniRef90_UPI001F1864BA" ], "nt": "ATGAATAATTTAAGTTATGATATAAAAGAACAAGAAAAAATTTTGAATGATATAAATTCTAAGATTTCTTTAATTGAAGAAAACTCATTTACAAACCAAGAATTAAAGAATACTTTTGCTAAATTAAAACAAAAATTATCAATATTGAATAAAAACTAA", "aa": "MNNLSYDIKEQEKILNDINSKISLIEENSFTNQELKNTFAKLKQKLSILNKN", "aa_hexdigest": "ea442a1070a8d630406992b797670cdd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 72910, "stop": 73206, "observations": { "mutation": [ 73045 ], "stop": [ 73045 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F1864BA", "query_cov": 1.0, "subject_cov": 0.5306122448979592, "identity": 0.981, "score": 94.0, "evalue": 6.06e-24, "gene_start": 1, "gene_end": 52, "reference_start": 47, "reference_end": 98, "reference_sequence": "MNNSKLDKQEIKDFKEKLSAKNLEIEKYLSSEETFKDYRAYNPDLWMNNLSYDIKEQEKILNDINSKISLIEENSFTNQELKNTFAELKQKLSILNKN" }, "paralog": false, "identity": 0.9591836734693877, "score": 186.0, "evalue": 6.94e-63, "description": "Internal stop codon at 73045. Nonsense mutation around 73045.", "psc": { "uniref90_id": "UniRef90_UPI001F1864BA", "uniref50_id": "UniRef50_UPI002735871D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F1864BA", "UniRef:UniRef50_UPI002735871D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002735871D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002735871D" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6098.8873, "isoelectric_point": 8.008730125427245 }, "id": "DFOKDJOJIG_161", "locus": "GCA013367855_00121" }, { "type": "cds", "sequence": "contig_1", "start": 73430, "stop": 73903, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAATTTTAGAACATTTTTATTTTAAAAGTCCTGAAACAAATAAAGATTATTTATTTTTTGAATATAATGACGAAACTATTTCAAAAGAATTTAAAGATATCGAAACTGAATTATTATCATATCAAAAGCAAAAACAAGATAAATTAGATAAATATTTATATAGTTTTACAATAAATCATGACACCGAAAATATAGATGAAGAAAAATTAATAAAATCTAATGACTATTATTTCAACCCTGAAGAAGACATGATGTATCTTCATTATCATTTCGGACAATTACTAGATCAAAAAATTAATTCAGTTTTTGAATATTACAATAAAAAAGCAAAAGCCTTATTACAAAAAATTCAAAATAACGCTAATTATTCAAAACAAAAAGAAAAATTGCAAAAATTGCTTTCAAAAAATATTTCTTATAGACTTATATTTTCGGGTGAAACAAATCGAGAAAATATCTACTATAATTGA", "aa": "MRILEHFYFKSPETNKDYLFFEYNDETISKEFKDIETELLSYQKQKQDKLDKYLYSFTINHDTENIDEEKLIKSNDYYFNPEEDMMYLHYHFGQLLDQKINSVFEYYNKKAKALLQKIQNNANYSKQKEKLQKLLSKNISYRLIFSGETNRENIYYN", "aa_hexdigest": "27f2dafd372a9a807bdf1f0b11127371", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19309.42190000001, "isoelectric_point": 5.850517082214354 }, "id": "DFOKDJOJIG_162", "locus": "GCA013367855_00122" }, { "type": "cds", "sequence": "contig_1", "start": 73922, "stop": 74047, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAAGAAAAATTTATTAATTTAAAACCCGCACAAATAAAGCAATTCTTTAAAGAATATGAAGAAAGAAAAGAAGCATTTTATAGAATTAATGAAATGGCGTTAGTTTTAGAAAGAACATGA", "aa": "MPKEKFINLKPAQIKQFFKEYEERKEAFYRINEMALVLERT", "aa_hexdigest": "a9f85296b5ae8063f8a66f9de5ce5be6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5107.946, "isoelectric_point": 9.156267356872558 }, "id": "DFOKDJOJIG_163", "locus": "GCA013367855_00123" }, { "type": "cds", "sequence": "contig_1", "start": 74072, "stop": 74755, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTCATTCTACAAAATAAATAGTATGAATTCAAAACTTAATAATGACATTTATATAAAAAATATTGAATTTTATGAATATGCAGAAAAAATCTGTGCTTCAGTCAAGCAACTAAAATCCTTGCATTCTAAATTAATTAATATAAAAGCAAATAACAATATAGAGCAAGATCTAGATTCATTAATATCAAAATGTGAAATCAGTATAAACAAAAACAAAAATATTGATAATAGCCTTAATTTAAATGACTTGAAAGAGAGATATTCTGAAATATCAGCAGCTATAAAAAACGTTTCAAATTCCTTAGGCCAATATGAAAATACAATATCAAAAAAGATTGCTGAATATAAAGAAAATATTTCAAAAATAGAAAACATTAAAAATAGTTATTTTTCACATATTGATAATGGGGAAGAATATTACAAATTGTTTAAAGATGAAATTGAATCATTTAAAAAGGATTTTAAAAAGATTCAATCTATAAAAAGTTATGATGAATATTCAAAAGAAACAGATGCTAAAATTAATTATTTAAAACAAGAAATTAAAGAACACGCTAGATTAAGCAAAGAAATTAAAGAAAATGGTGAAAACAAAGTAAATAAAGATTCTGGCGAAGACACATTTTTGGAACTTAGAAACTCAAAATTCAAAGAACCAAAATCATTTTTATTACTTTGA", "aa": "MDSFYKINSMNSKLNNDIYIKNIEFYEYAEKICASVKQLKSLHSKLINIKANNNIEQDLDSLISKCEISINKNKNIDNSLNLNDLKERYSEISAAIKNVSNSLGQYENTISKKIAEYKENISKIENIKNSYFSHIDNGEEYYKLFKDEIESFKKDFKKIQSIKSYDEYSKETDAKINYLKQEIKEHARLSKEIKENGENKVNKDSGEDTFLELRNSKFKEPKSFLLL", "aa_hexdigest": "43a55e6fc9e8b6c45dc73cad50232b21", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26609.681600000033, "isoelectric_point": 7.617982292175294 }, "id": "DFOKDJOJIG_164", "locus": "GCA013367855_00124" }, { "type": "cds", "sequence": "contig_1", "start": 75088, "stop": 75675, "strand": "-", "frame": 1, "gene": "tdk", "product": "thymidine kinase", "db_xrefs": [ "COG:COG1435", "COG:F", "EC:2.7.1.21", "GO:0004797", "GO:0005524", "RefSeq:WP_073673795.1", "SO:0001217", "UniParc:UPI00093D3F1C", "UniRef:UniRef100_UPI00093D3F1C", "UniRef:UniRef50_Q5ZZT0", "UniRef:UniRef90_D1J7J2" ], "nt": "ATGTATAAAACATTTAATGATGGAATGATAGAAGTTATCACTGGACCAATGTTTAGTGGAAAAAGTGAAGAATTATTAAGGAGAGTTAGAACCTTAGAATATGCTAAGATGCTACCGCTTTTAATAAAGCCCGAATATGATAATCGCTTTTCTGATGATGAAATAGTAAGCCGTTGTGGTGTAAGAAGAAAAACGCATTCATTAAAAAACATCAATGAAGTTTATGAATTACTTAAACAAGATAAATATAAGGCAATAATAATTGACGAAGCCCATTTTTTTGGGAATTCATTAATTGAAGTCGCTGATGACTTAGCAAATAAGGGATATTTAGTAATTGTAGCTGGCCTAGACCAAGATTATCTAAGAAAACCTTTTGGGCCAATTCCTGCCTTAATGGCAATGGCTGAACGAGTAACTAAATTGCAAGCAATTTGTGTAGTATGTCAACATGCTGCTTCAACAAGTTTTAGAAAAGATTCTTCAAATGAACAAAACTTAATTGGTGACTATAATGAATATGAAGCAAGATGCAGAAAGTGCCACATAATAGGTCAAAAACAAAAGGAATTACTAAAAAATAAGTAA", "aa": "MYKTFNDGMIEVITGPMFSGKSEELLRRVRTLEYAKMLPLLIKPEYDNRFSDDEIVSRCGVRRKTHSLKNINEVYELLKQDKYKAIIIDEAHFFGNSLIEVADDLANKGYLVIVAGLDQDYLRKPFGPIPALMAMAERVTKLQAICVVCQHAASTSFRKDSSNEQNLIGDYNEYEARCRKCHIIGQKQKELLKNK", "aa_hexdigest": "47fc38de18b54b1b70db1b6c9c8cb3d3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00093D3F1C", "ncbi_nrp_id": "WP_073673795.1", "uniref100_id": "UniRef100_UPI00093D3F1C", "db_xrefs": [ "SO:0001217", "UniParc:UPI00093D3F1C", "RefSeq:WP_073673795.1", "UniRef:UniRef100_UPI00093D3F1C" ] }, "ips": { "uniref100_id": "UniRef100_UPI00093D3F1C", "uniref90_id": "UniRef90_D1J7J2" }, "psc": { "uniref90_id": "UniRef90_D1J7J2", "gene": "Tdk", "product": "thymidine kinase", "ec_ids": [ "2.7.1.21" ], "uniref50_id": "UniRef50_Q5ZZT0", "cog_id": "COG1435", "cog_category": "F", "go_ids": [ "GO:0004797", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_Q5ZZT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5ZZT0" ], "product": "Thymidine kinase" }, "genes": [ "tdk" ], "id": "DFOKDJOJIG_165", "locus": "GCA013367855_00125" }, { "type": "cds", "sequence": "contig_1", "start": 75750, "stop": 76640, "strand": "+", "frame": 3, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "COG:COG0012", "COG:J", "GO:0005524", "GO:0005525", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47270", "UniRef:UniRef90_D1J7J3" ], "nt": "ATGAGTTTAAAAGCTGGTATAGTTGGATTGCCTAATGTTGGCAAAAGTACATTATTTTCTGCATTAACATTAAATGAAGCTGAAATGGCAAATTATGCTTTTACTACAATTGAGCCTAACGTTGCAATAGTTAATTTAGAAGATCCAAGGCTTTATGAATTAGCTAAAATAGTTCAAACAGAAAAAATTGTTCCTGCTACTTTTCAATTTGTTGATATAGCTGGATTAGTTGCTGGGGCTTCAAAAGGAGAAGGCTTAGGCAATAAGTTTTTAGCAAATATTCGCGAAGTTGATGCAATTGTTCATGTTATAAGATGCTTCGAAAATGCTGATATAATGCATGTTAACAATAAAATTGATCCTATTAGTGATTTAAAAACTATTAATATTGAATTAATATTGGCAGATTTGCAAACTATTGAAAATGTAATTAATAGAATTGGCAAAAAAGCTCAAAATTCAGGTGATAAAGAATTAAAATTCGAATTTGAAGTTGCCTTAAAAATACGTAATGCGTTAGAAAATGAAATTATGGTTAAAAATATGGATTTAAGTTCAGAAGAACAAGCTGTGATCAAATCATATCAATTGCTTACTGCAAAACCTACAATCTATGTTGCAAACCTTGATAGTGATAGTTTTAAAAATCTAGAAAGTGCTAAATATTATCAAGAATTAAACAAATATTTGCAAGACAAAAAAGAAATTTTAATACCTATTTGTATTAAATTAGAATATGAATTATGTAAATTTAATGATGAAGAAAAAGAAATGTTTTTAAATGAATATAACATTGAATGCTCCGGATTAAATTTAATTATCAAGAAAAGTTTTTATTTATTAAATCAAGCAACATATTTTACGGCGGGTAAAATCGAAGCAAGGGCATGA", "aa": "MSLKAGIVGLPNVGKSTLFSALTLNEAEMANYAFTTIEPNVAIVNLEDPRLYELAKIVQTEKIVPATFQFVDIAGLVAGASKGEGLGNKFLANIREVDAIVHVIRCFENADIMHVNNKIDPISDLKTINIELILADLQTIENVINRIGKKAQNSGDKELKFEFEVALKIRNALENEIMVKNMDLSSEEQAVIKSYQLLTAKPTIYVANLDSDSFKNLESAKYYQELNKYLQDKKEILIPICIKLEYELCKFNDEEKEMFLNEYNIECSGLNLIIKKSFYLLNQATYFTAGKIEARA", "aa_hexdigest": "89728643451e2edb9fba7656fd09ba93", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J7J3", "query_cov": 1.0, "subject_cov": 0.8065395095367848, "identity": 0.997, "score": 567.0, "evalue": 7.59e-203, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P47270", "cog_id": "COG0012", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47270" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "DFOKDJOJIG_166", "locus": "GCA013367855_00126" }, { "type": "cds", "sequence": "contig_1", "start": 76656, "stop": 76853, "strand": "+", "frame": 3, "gene": "ychF", "product": "YchF C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A382QXD8" ], "nt": "ATGAATGCTGCTCAATGTGCAGGAATTATTCACACTGATTTTGAAAAGAAATTTGTAAAAGCAGAAGTCATTAAATATGAAGATTATATTGCTTTAAATGGCGAAAAAGGTTGTAGAGAAGCTGGAAAAATAGCAATTGAGGGCAAGAATTATATTATGCAAGATGGTGATGTTTGCTTTTTTAGAGTGGCAAAATAA", "aa": "MNAAQCAGIIHTDFEKKFVKAEVIKYEDYIALNGEKGCREAGKIAIEGKNYIMQDGDVCFFRVAK", "aa_hexdigest": "0d1a58d84e5a83662e91b060d95673aa", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A380DK80", "query_cov": 0.9538461538461539, "subject_cov": 0.8051948051948052, "identity": 0.613, "score": 89.4, "evalue": 3.96e-22, "valid": false, "product": "GTP-binding and nucleic acid-binding protein YchF", "uniref50_id": "UniRef50_A0A382QXD8" }, "pscc": { "uniref50_id": "UniRef50_A0A382QXD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A382QXD8" ], "product": "YchF C-terminal domain-containing protein" }, "genes": [ "ychF" ], "id": "DFOKDJOJIG_167", "locus": "GCA013367855_00127" }, { "type": "cds", "sequence": "contig_1", "start": 77178, "stop": 77747, "strand": "+", "frame": 3, "gene": null, "product": "Polymerase/histidinol phosphatase N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L9U4", "UniRef:UniRef90_D1J7J4" ], "nt": "ATGAATTTAATAAAAATTGATTTGCATGTACATTCTCAGGATTCAAGCCATACAGGAAGTAATATTTCTCAAGAATCTGATTTAGAAAAATTGTTAATTTTAACTAAAAAAAGAGTCAAAGTTGCTAGTTTTTCTGACCATGATAATTTTTATATTGAAAGTTATTTGAAACGTCTTGAAATAATCAAACAATACAATATTGATATTACATTATGGCCTGGTATTGAAGTTAATTTAAGAAAATATGATGGTCAAATAGGACAAGCTATATTTATATTTGATCCAGATAGCAACTTAAAAGAATTAAATGAAGTAACTAAAAAGGAATTTAGATTCAATAGCAATAAATACACATACAAAGACGCTGTTGAACTATTTAAAAAAAGCAACTTCAATTTTATGGTTTTCCCTCATGCTGGAAAAGGTCAAGATAATATGGAATGGCAAGATATATGCAATAGCCAAGTTGATGGGCTGGACATAACAGATTTTAATAATAGCAATAAAAAGAAAATATTAAAACAAGATCCAAACATTCCAGTTTTATATTTTAGTGACACACATACATGA", "aa": "MNLIKIDLHVHSQDSSHTGSNISQESDLEKLLILTKKRVKVASFSDHDNFYIESYLKRLEIIKQYNIDITLWPGIEVNLRKYDGQIGQAIFIFDPDSNLKELNEVTKKEFRFNSNKYTYKDAVELFKKSNFNFMVFPHAGKGQDNMEWQDICNSQVDGLDITDFNNSNKKKILKQDPNIPVLYFSDTHT", "aa_hexdigest": "932d0a76299056db6b9a3cb3e639f71a", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7J4", "query_cov": 1.0, "subject_cov": 0.8217391304347826, "identity": 0.995, "score": 382.0, "evalue": 6.75e-134, "valid": true, "product": "Polymerase/histidinol phosphatase N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A077L9U4" }, "pscc": { "uniref50_id": "UniRef50_A0A077L9U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L9U4" ], "product": "Polymerase/histidinol phosphatase N-terminal domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_168", "locus": "GCA013367855_00128" }, { "type": "cds", "sequence": "contig_1", "start": 77882, "stop": 78049, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13476.12" ], "nt": "ATGATAAAAAGCCTCAAAATTAAAAATAATAACGAAACTTATGACATAAATTTAAATAATCAAGTTAATGTTATTGTTGGTGAAAAGGGTTCTGGTAAAAGCACCTTGCTTTTGATTTTAGCAGAAGCAATAGTAAACAAAAAATTATTCAAAAACGAATATGAATGA", "aa": "MIKSLKIKNNNETYDINLNNQVNVIVGEKGSGKSTLLLILAEAIVNKKLFKNEYE", "aa_hexdigest": "6a444988ddbae595e6e1b6cf9a716b5e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13476.12", "name": "AAA_23", "length": 28, "aa_cov": 0.509090909090909, "hmm_cov": 0.9285714285714286, "evalue": 1.0522238534984048e-07, "score": 28.387985229492188, "start": 13, "stop": 40 } ], "seq_stats": { "molecular_weight": 6208.142099999999, "isoelectric_point": 9.050087928771973 }, "id": "DFOKDJOJIG_169", "locus": "GCA013367855_00129" }, { "type": "cds", "sequence": "contig_1", "start": 78320, "stop": 78913, "strand": "+", "frame": 2, "gene": null, "product": "AAA family ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491A3C", "UniRef:UniRef90_UPI001E491A3C" ], "nt": "ATGTTAAAACAATTAGATGATTTTAGATTGCTAGAAGATTTGTTAAATGAATATAGGCGCGAACGCGATAGGCAGATAGCACTTTCAAATGTTTTTAATCAATCTTTTAATTTAAATGAAGGTAGTACTAAAAACATTGCAACCATTGACTATGATTCTTCAAATGAAATAAAAAAAATTAAGGAATTATTGAAATCAATTGAAGAAACAAAAGAAAAAATATATAGTACAAATTTGGTTGTCAATAATCTTTTGGCACAATTTGAACAATCTTCGTCGCTACTTCAAATTTCAAATGAACAATTATTAGATATTCAACAAAGAACAGAAAATGTTATACTACAAAATAACTTATTTCTAGAATATTACAATCAATATAATTTAGTTGTATCCAAGGATAAAAAAGCTCTTGAATGTTTTAAATTATCATTTACAAATGCTAAAAGAGCATATCTTGAAGAATTAAGCAACACTTTAAAGATTCAAAATGATAATGAACAATTATTGGAACATTTTTCTAATTTAGGAAGAATAATGAAAAAAATCAATAGGCAATATAATTCAATAATTGAAAAAGAAATTATTTTAAACTGA", "aa": "MLKQLDDFRLLEDLLNEYRRERDRQIALSNVFNQSFNLNEGSTKNIATIDYDSSNEIKKIKELLKSIEETKEKIYSTNLVVNNLLAQFEQSSSLLQISNEQLLDIQQRTENVILQNNLFLEYYNQYNLVVSKDKKALECFKLSFTNAKRAYLEELSNTLKIQNDNEQLLEHFSNLGRIMKKINRQYNSIIEKEIILN", "aa_hexdigest": "d4fb4c96a88565c6afafb0a75a24af59", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 78020, "stop": 78940, "observations": { "mutation": [ 78047, 78911 ], "stop": [ 78047, 78911 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E491A3C", "query_cov": 1.0, "subject_cov": 0.5549295774647888, "identity": 1.0, "score": 362.0, "evalue": 5.95e-124, "gene_start": 1, "gene_end": 197, "reference_start": 147, "reference_end": 343, "reference_sequence": "MIKSLKIKNNNETYDINLNSQVNVIVGEKGSGKSTLLLILAEAIVNKKLFKNEYEWFNEKAKFLVDSINIDNTEIKTQDFSYIFDSENKQNSKDAGILEIQKNLSGYISQNDSRKNSLDSSEYVEKQKSNCIDNFTEKIYYDQDQKMLKQLDDFRLLEDLLNEYRRERDRQIALSNVFNQSFNLNEGSTKNIATIDYDSSNEIKKIKELLKSIEETKEKIYSTNLVVNNLLAQFEQSSSLLQISNEQLLDIQQRTENVILQNNLFLEYYNQYNLVVSKDKKALECFKLSFTNAKRAYLEELSNTLKIQNDNEQLLEHFSNLGRIMKKINRQYNSIIEKEIILNWTLEETDKKIRI" }, "paralog": false, "identity": 0.9901960784313726, "score": 587.0, "evalue": 6.59e-215, "description": "Internal stop codon at 78047, 78911. Nonsense mutation around 78047, 78911.", "psc": { "uniref90_id": "UniRef90_UPI001E491A3C", "product": "AAA family ATPase", "uniref50_id": "UniRef50_UPI001E491A3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E491A3C", "UniRef:UniRef50_UPI001E491A3C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E491A3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491A3C" ], "product": "AAA family ATPase" } }, "id": "DFOKDJOJIG_170", "locus": "GCA013367855_00130" }, { "type": "cds", "sequence": "contig_1", "start": 79091, "stop": 79561, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E54A28D", "UniRef:UniRef90_UPI001E54A28D" ], "nt": "ATGATTAAGGCATTGATTAAAGATAAAATAAAAATCTATGCAGGGGATGAAGAATATAAAAATCTATCAATGGGCCAAAAGACATTGTTTGGTATAACACACGCGATTGACACATTGAACAATGTTCCTAATGAACAATACTTATTGCTAGACCAAATTGAAGATAATCTAGATAATAAAACAATATACGAAAAAATAGTTCCAATGTTGCAAGAACAAATTAAAAAAGGCAAACAAATTTTTATCGTTACACATAACCCTAATATTGGAACATTGATAAAAGGCAATATAATAACAACAGATATTTTCAATACTAATCTAAACAAAAAATTTGTTGTTAATGAAATAATTCGCGAAGATAATGAAATTGACACACCTCAATCGCTTTATTTGGAGGGCAGTATGAAAGCCTTATATGAAAGATTAAAAGTACATGAAGAAAAAATTAATAAAACCAAAGGAGATAAATAA", "aa": "MIKALIKDKIKIYAGDEEYKNLSMGQKTLFGITHAIDTLNNVPNEQYLLLDQIEDNLDNKTIYEKIVPMLQEQIKKGKQIFIVTHNPNIGTLIKGNIITTDIFNTNLNKKFVVNEIIREDNEIDTPQSLYLEGSMKALYERLKVHEEKINKTKGDK", "aa_hexdigest": "d9a73df02f816b5e07e39b01a3129460", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E54A28D", "query_cov": 1.0, "subject_cov": 0.8387096774193549, "identity": 1.0, "score": 305.0, "evalue": 1.62e-104, "valid": true, "product": "ATP-binding protein", "uniref50_id": "UniRef50_UPI001E54A28D" }, "pscc": { "uniref50_id": "UniRef50_UPI001E54A28D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E54A28D" ], "product": "ATP-binding protein" }, "genes": [], "id": "DFOKDJOJIG_171", "locus": "GCA013367855_00131" }, { "type": "cds", "sequence": "contig_1", "start": 79561, "stop": 79683, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7J6", "UniRef:UniRef90_D1J7J6" ], "nt": "ATGATAACAATTGACTATGTATTTACAAAAGATGAAAAGAGATTAATAGTTATTTCTAATGCAAGCGATAGCAAAAATAAATATAAAATTGAAATTGACTTAGACAACCCTTCTGATGCTTGA", "aa": "MITIDYVFTKDEKRLIVISNASDSKNKYKIEIDLDNPSDA", "aa_hexdigest": "0b5c43c96b7a5c397768c5edef3831e1", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 79561, "stop": 79857, "observations": { "mutation": [ 79681 ], "stop": [ 79681 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7J6", "query_cov": 1.0, "subject_cov": 0.40816326530612246, "identity": 1.0, "score": 78.2, "evalue": 6.29e-18, "gene_start": 1, "gene_end": 40, "reference_start": 1, "reference_end": 40, "reference_sequence": "MITIDYVFTKDEKRLIVISNASDSKNKYKIEIDLDNPSDAWNKENINNFIIRAISISDEKLSEPQLTESAQEQLQKGNKQIEFIKNLFTNFVERYNEN" }, "paralog": false, "identity": 0.9897959183673469, "score": 190.0, "evalue": 1.4e-64, "description": "Internal stop codon at 79681. Nonsense mutation around 79681.", "psc": { "uniref90_id": "UniRef90_D1J7J6", "uniref50_id": "UniRef50_D1J7J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7J6", "UniRef:UniRef50_D1J7J6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7J6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4604.149700000001, "isoelectric_point": 4.663718986511231 }, "id": "DFOKDJOJIG_172", "locus": "GCA013367855_00132" }, { "type": "cds", "sequence": "contig_1", "start": 79964, "stop": 80488, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGAAGAATGGTAAAGAAGTTAAAATGTCAAAACGTTTAGGAACTTCTTTAACGTTAAGAGAACTAGCAGAAGAAGTTGGAAATGATGCAATTAGATACTTTCTAATTGATAGATCATATAATTCAAGAATTGACTTTGATATTAACAAAGTTACTGACAACAACGAAAATAACCCTATGTATATCATTAAATATGCTCATGCTCGTGCTTGCCAATTATTAGATAAAGTAGGAATTGAAAACCCTATTGCTTCTGATGAGTTGGATAATGAATATGCTCAAAAGCTTGTTGCAGAATTAAAAGAATACCCTGAATTAATTTCAACAATGGCCAAGACATATAAAGTCAACTTGTTGCCTCCTTATCTATTAAAATTATCAAAGGCATTTAACTCATTTTATTCAAATACCAAAGTTTTAGGATCTCCTAACGAACAAAGCCTTGCAGCTTTAGTAAAGGCAACAAAAATTGTTTTAGCTGATGGTTTAAAATTAATGGACATTGAAGCACCTGAAAAAATGTAA", "aa": "MKNGKEVKMSKRLGTSLTLRELAEEVGNDAIRYFLIDRSYNSRIDFDINKVTDNNENNPMYIIKYAHARACQLLDKVGIENPIASDELDNEYAQKLVAELKEYPELISTMAKTYKVNLLPPYLLKLSKAFNSFYSNTKVLGSPNEQSLAALVKATKIVLADGLKLMDIEAPEKM", "aa_hexdigest": "cd944fc5de318be4932856bb9ea673b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 117, "aa_cov": 0.6436781609195402, "hmm_cov": 1.0, "evalue": 1.8195797235781623e-29, "score": 97.86124420166016, "start": 63, "stop": 174 } ], "seq_stats": { "molecular_weight": 19667.477199999998, "isoelectric_point": 6.618639183044434 }, "id": "DFOKDJOJIG_173", "locus": "GCA013367855_00133" }, { "type": "cds", "sequence": "contig_1", "start": 80795, "stop": 81229, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTGCTAACCCAACAGGATATTTGCATATTGGACACGCAAGAAATGCTGCTATTGGTGCAACATTGTGCAATATTCTAGAAAAAGCAGGTCACCGCGTTGTTCGAGAATATTTAGTTAATGATTATGGAAACCAAATGAATAAAATGGCAGATTCAATATTTGCTAGATACCAACAAATATTTAATCCTGAATTTCCTATGCCAGAAGATTCATACCATGGCGGAGATATGATAGAATTCGCTCAAGCATTTTATGACATTCACAAAGATGAATACAAAAATGTAGAATACACTGATGAAATTAGAAAAATCTTTAGAAGTTTTGGAAGAGATTTTGCTTTAAAAAATATTATTGAAGATATGAAAAAATTTGGAGTAACATTCGATATTTTTACATCAGAAGCAGAACAATATGCCAAAAATAGAGTATGA", "aa": "MSANPTGYLHIGHARNAAIGATLCNILEKAGHRVVREYLVNDYGNQMNKMADSIFARYQQIFNPEFPMPEDSYHGGDMIEFAQAFYDIHKDEYKNVEYTDEIRKIFRSFGRDFALKNIIEDMKKFGVTFDIFTSEAEQYAKNRV", "aa_hexdigest": "413d6417d54599f2187c93b44cd613f0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16722.643999999993, "isoelectric_point": 5.709272193908691 }, "id": "DFOKDJOJIG_174", "locus": "GCA013367855_00134" }, { "type": "cds", "sequence": "contig_1", "start": 81335, "stop": 81589, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03485.21" ], "nt": "ATGAAAACAACGAAAGACATTATTATTGCTTCATTAAATGAAGCACTTGAAAAATTAAATATTTCTAAGCCTATTGTACTTACCGAATCTAAAAATTACGGCGACTATTCAAGCAATATTGCCCTAACATTGCAAAAGGATTTAGGTAAGAAAGCAATAGATATTGCCCAAGAAATAGTAAGAAATATTGATTTAAATAAATTTAAAGAAATTAGTGAAATAAAGGTTGCCGAACCTGGTTTTATTAATTTTTGA", "aa": "MKTTKDIIIASLNEALEKLNISKPIVLTESKNYGDYSSNIALTLQKDLGKKAIDIAQEIVRNIDLNKFKEISEIKVAEPGFINF", "aa_hexdigest": "c4fb2651a79feec6cd8afa5b3577dae8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03485.21", "name": "Arg_tRNA_synt_N", "length": 81, "aa_cov": 0.9404761904761905, "hmm_cov": 0.9753086419753086, "evalue": 9.862101177994038e-20, "score": 66.67340087890625, "start": 6, "stop": 84 } ], "seq_stats": { "molecular_weight": 9409.805399999996, "isoelectric_point": 6.232872962951659 }, "id": "DFOKDJOJIG_175", "locus": "GCA013367855_00135" }, { "type": "cds", "sequence": "contig_1", "start": 81650, "stop": 82132, "strand": "+", "frame": 2, "gene": null, "product": "Cysteine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "nt": "ATGAAAAGATATTATTTATGTGGCCCAACCGTTTATAACTATCCCCATATAGGCAATTTAAGGCCAATAGTTAGTTTTGATTTAATGATTAGGGCTCAAAGGCAATTGGGAGAAGATGTTTATTTTCTTAACAATATCACTGATATAGATGACAAAATCATTGCTAAGGCAAAACAAGAAAACAAGACAGAAAAAGAAATTTCAAGTTTTTATGAAGCATATTATTTAAATTTATTTAAATTATTCTCAATTGAAATGCCAAATAAAATTGTAAGAGTTACGGATTCCCTTAATGATATGTATGAATATATTCAATCATTGATTGAAAAGGGCGCAGCATATCAAGTGAACGGCAATGTCTATTTCGACGTTTTGAAATTTAAAGATGTGTATGGAAAAATAAGCAACCAGAAATTGGCAAATTTAATTTCAGATTCAAGCAATGAAGTTGACAAGCGTTCGCCATATGATTTCGCTTTATGA", "aa": "MKRYYLCGPTVYNYPHIGNLRPIVSFDLMIRAQRQLGEDVYFLNNITDIDDKIIAKAKQENKTEKEISSFYEAYYLNLFKLFSIEMPNKIVRVTDSLNDMYEYIQSLIEKGAAYQVNGNVYFDVLKFKDVYGKISNQKLANLISDSSNEVDKRSPYDFAL", "aa_hexdigest": "80306df1ac46f81b54a97964c961b2a3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZZN9", "query_cov": 0.8, "subject_cov": 0.9624060150375939, "identity": 0.664, "score": 186.0, "evalue": 5.4e-58, "valid": false, "product": "Cysteine--tRNA ligase", "uniref50_id": "UniRef50_A0A448ZZN9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "product": "Cysteine--tRNA ligase" }, "genes": [], "id": "DFOKDJOJIG_176", "locus": "GCA013367855_00136" }, { "type": "cds", "sequence": "contig_1", "start": 82358, "stop": 82777, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF01406.26" ], "nt": "TTGAATTATAAGAATCAAAAGATGTCTAAGTCAATAGGAAACATAATTTATGCTGAAGAATTTTTAAAGAATTATTCAGCTGATACTTATAAATTGTTAATGCTATCAACAAGTTACAGCAAACCAATTAATATTACCGATGAATTACTTGCATCGCTTCAAAAACAAATTAATAATATTGAGGCGAATTTTAATAAAAGTCAATTGGAAGATATTAAATATACGTTAGATAAACAAATAATAGATTCAATTTTAAAAAATGTTTCTAATCTTGATTTTTCATTAGCCTTTAAGCAACTTAATGAGCAATTAAAATCACCATTGAATTATGGTAATGCATTTTTTGTATTTAAGGTTTTAGGTTTTAAATTAGTTGAAAATAAAGTAAGCAATGATGATAAAGAACTATATAGAAAGTGA", "aa": "MNYKNQKMSKSIGNIIYAEEFLKNYSADTYKLLMLSTSYSKPINITDELLASLQKQINNIEANFNKSQLEDIKYTLDKQIIDSILKNVSNLDFSLAFKQLNEQLKSPLNYGNAFFVFKVLGFKLVENKVSNDDKELYRK", "aa_hexdigest": "9f81eeb86b5de26e476d80848706616d", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 42, "aa_cov": 0.302158273381295, "hmm_cov": 1.0, "evalue": 1.312265702151227e-07, "score": 26.317541122436523, "start": 4, "stop": 45 }, { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 51, "aa_cov": 0.3669064748201439, "hmm_cov": 1.0, "evalue": 3.100816375470776e-10, "score": 35.5084228515625, "start": 2, "stop": 52 } ], "seq_stats": { "molecular_weight": 16139.32119999999, "isoelectric_point": 8.923472023010255 }, "id": "DFOKDJOJIG_177", "locus": "GCA013367855_00137" }, { "type": "cds", "sequence": "contig_1", "start": 82855, "stop": 83322, "strand": "+", "frame": 1, "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "db_xrefs": [ "COG:COG0566", "COG:J", "GO:0006396", "GO:0008173", "SO:0001217", "UniRef:UniRef50_C4XFX7", "UniRef:UniRef90_D1J7J9" ], "nt": "ATGAAAAAAATAGTTTTTGGAAAAAATTCAGTCTTAGAAGCAATTGAATATGAATTTCCTATAGAAGAGATTTACTTGCAAAAAGGACTTGTTAACAACAATTTAAAATTTAAGAAAGTTGTTTATTTAGATAGAAATAGCCTAGATAAATTAACAGAAAATGGAAATCACCAAGGATATGCTGCAAAATTAAAAGATTTTCAATATCATGATTTGGGCAGTATTTTTAAAGACGGCTCAGATAAGGTTTTAATTTTAGACCATATCCAAGACCCTCAAAATTTTGGTGCTATTATACGTAGTGCCAATGTGTTTGGCTTTAAACATATAATCATTCCAAAGGATAGAGCATGTGATGTAACTCCGAGTGTTTTAAAAACTAGCTCGGGAGGTTTTAAAAACGTTAAAGTGATTAAAGTTAACAGTTTATTTGATGCAATTGAGGAATTAAAAAATAAAGGTTTTTGA", "aa": "MKKIVFGKNSVLEAIEYEFPIEEIYLQKGLVNNNLKFKKVVYLDRNSLDKLTENGNHQGYAAKLKDFQYHDLGSIFKDGSDKVLILDHIQDPQNFGAIIRSANVFGFKHIIIPKDRACDVTPSVLKTSSGGFKNVKVIKVNSLFDAIEELKNKGF", "aa_hexdigest": "1e54981a63b0844ac6a0f58473b6586c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rlmB" ], "truncated": "3-prime", "pseudogene": { "start": 82855, "stop": 83547, "observations": { "mutation": [ 83320 ], "stop": [ 83320 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7J9", "query_cov": 1.0, "subject_cov": 0.6739130434782609, "identity": 1.0, "score": 313.0, "evalue": 6.4e-107, "gene_start": 1, "gene_end": 155, "reference_start": 1, "reference_end": 155, "reference_sequence": "MKKIVFGKNSVLEAIEYEFPIEEIYLQKGLVNNNLKFKKVVYLDRNSLDKLTENGNHQGYAAKLKDFQYHDLGSIFKDGSDKVLILDHIQDPQNFGAIIRSANVFGFKHIIIPKDRACDVTPSVLKTSSGGFKNVKVIKVNSLFDAIEELKNKGFWIYASALNQQAKKLNDAKFNEKIAIIVGNEGSGVSKTLLKHSDEIIYIEQDKESVQSLNVSVATGILLYTVFNKK" }, "paralog": false, "identity": 0.9956521739130435, "score": 449.0, "evalue": 6.01e-163, "description": "Internal stop codon at 83320. Nonsense mutation around 83320.", "psc": { "uniref90_id": "UniRef90_D1J7J9", "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "uniref50_id": "UniRef50_C4XFX7", "cog_id": "COG0566", "cog_category": "J", "go_ids": [ "GO:0006396", "GO:0008173" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7J9", "UniRef:UniRef50_C4XFX7", "COG:COG0566", "COG:J", "GO:0006396", "GO:0008173" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFX7" ], "product": "RNA 2-O ribose methyltransferase substrate binding domain-containing protein" } }, "id": "DFOKDJOJIG_178", "locus": "GCA013367855_00138" }, { "type": "cds", "sequence": "contig_1", "start": 83615, "stop": 84550, "strand": "-", "frame": 3, "gene": "arcC", "product": "Carbamate kinase", "db_xrefs": [ "COG:COG0549", "COG:E", "RefSeq:WP_175972187.1", "SO:0001217", "UniParc:UPI00158C8080", "UniRef:UniRef100_UPI00158C8080", "UniRef:UniRef50_P78030", "UniRef:UniRef90_D1J7K0" ], "nt": "ATGAGTAGAATTGTTATTGCATTAGGCGGAAACGCTTTAGGAAATAACCCCGAAGAACAAAAAGAACTTGTTAAAGTTCCTGCAGCCAAAATTGCTGAATTAGTAAAAGCAGGACATGAAGTTATTGTTGGACACGGAAACGGACCACAAGTTGGAATGATTTTCAATGGTTTCGTTAGTGCTCACTCAGTTAATCCTAAGTCTCCTTTAGTTCCATTGCCTGAAGCAGGTGCAATGAGCCAAGGATACATTGGATATCATATGGTTAATGCTATTACAAATGCATTTAAAAACGAAGGATTAAAGAACAAAGAAGTTTTATACATTTTAACTCAAACTCTTGTAGATCCAAAAGACCTAGCATTCCAAAATCCTACAAAACCAATTGGCCCATTCTTTGCAACAGAAGCAGAAGCAGTTGCTGCAAACCCAAATAGCACAATTGTTGAAGATGCAGGTAGAGGCTACAGAAAAGTTGTAGCATCTCCAAAACCATTAAACTTTGTTGGTATTAATCAAATTAAAAAAGCTATTGAATCAGGTGCTACCGTTATCGTTGGTGGTGGTGGTGGAATACCTACTGTTGAAGATGAAAATGGCTTTATTAAAGGTGTAGATGGTGTTATTGACAAAGATTTCGCTTTAGCAAAAATGGCTGCATTAGCAAAAGCTGATTACTTTATTGTTCTAACAGCAGTTGATTATGTTAAAGTAAATTACAACAAGCCAGATCAAAAAGATCTAAAACACGCTACAAAAGCAGAACTTGAAAAATACATTGCTGAAAAACAATTTGCCCCAGGATCAATGTTACCAAAGGTACAAGCAGCTATTAAATTCGTTGAAGAAGGCGGAAAAGCAGCATTTATTGGTGATCTAAAAGATCTTGAAAACATTCTAGCAGAAAAAATCGGTACAAAAGTTACTTTAAATTAA", "aa": "MSRIVIALGGNALGNNPEEQKELVKVPAAKIAELVKAGHEVIVGHGNGPQVGMIFNGFVSAHSVNPKSPLVPLPEAGAMSQGYIGYHMVNAITNAFKNEGLKNKEVLYILTQTLVDPKDLAFQNPTKPIGPFFATEAEAVAANPNSTIVEDAGRGYRKVVASPKPLNFVGINQIKKAIESGATVIVGGGGGIPTVEDENGFIKGVDGVIDKDFALAKMAALAKADYFIVLTAVDYVKVNYNKPDQKDLKHATKAELEKYIAEKQFAPGSMLPKVQAAIKFVEEGGKAAFIGDLKDLENILAEKIGTKVTLN", "aa_hexdigest": "ecc0262976b5f075e7a670e3162c91f6", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158C8080", "ncbi_nrp_id": "WP_175972187.1", "uniref100_id": "UniRef100_UPI00158C8080", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C8080", "RefSeq:WP_175972187.1", "UniRef:UniRef100_UPI00158C8080" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C8080", "uniref90_id": "UniRef90_D1J7K0" }, "psc": { "uniref90_id": "UniRef90_D1J7K0", "gene": "ArcC", "product": "Carbamate kinase", "uniref50_id": "UniRef50_P78030", "cog_id": "COG0549", "cog_category": "E" }, "pscc": { "uniref50_id": "UniRef50_P78030", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78030" ], "product": "Carbamate kinase-like protein" }, "genes": [ "arcC" ], "id": "DFOKDJOJIG_179", "locus": "GCA013367855_00139" }, { "type": "cds", "sequence": "contig_1", "start": 84552, "stop": 84800, "strand": "-", "frame": 2, "gene": null, "product": "Ornithine carbamoyltransferase chain F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "nt": "ATGGCAATGATCAAAGCAGCAAAAGAAAATGTTATCTTCCTACACTGCTTACCAGCATTCCACGATGACCAAACTCAATTCTCAGCAACTATTAAAGAAACCTTAGGTGCAAAATACCCAGTTGTTTCAACCGGTGCTATGGAAGTTACTGATGAAGTATTCCAATCAAAATACAACAAATCAATCGAACAAGCTGGAAACCGTATGCACACAATTAAGGCTGTTATTCTAGCTACTTTAGGATACTAA", "aa": "MAMIKAAKENVIFLHCLPAFHDDQTQFSATIKETLGAKYPVVSTGAMEVTDEVFQSKYNKSIEQAGNRMHTIKAVILATLGY", "aa_hexdigest": "0e8f710bbe858e2fe23fe497b4f89f6b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4U9HLN2", "query_cov": 0.9634146341463414, "subject_cov": 0.9080459770114943, "identity": 0.519, "score": 75.1, "evalue": 4.39e-16, "valid": false, "product": "Ornithine carbamoyltransferase chain F", "uniref50_id": "UniRef50_A0A4U9HLN2" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9HLN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "product": "Ornithine carbamoyltransferase chain F" }, "genes": [], "id": "DFOKDJOJIG_180", "locus": "GCA013367855_00140" }, { "type": "cds", "sequence": "contig_1", "start": 85014, "stop": 85175, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00185.30" ], "nt": "ATGCTAGCTGACTACTTAACATTTAAAGAACACGTTGGAGACCTAAGAGGAAAGAAACTTGTTTTCTCAGGTGATATTAAAAACAACGTTGCTCGTTCATTAATGATCGGTGCTGCATTCTTTGGTGTTAATATTGTATTATGTGGACCAAAAGCTCAATGA", "aa": "MLADYLTFKEHVGDLRGKKLVFSGDIKNNVARSLMIGAAFFGVNIVLCGPKAQ", "aa_hexdigest": "ec6f9a72f8a919aca53a992059886de7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00185.30", "name": "OTCace", "length": 36, "aa_cov": 0.6792452830188679, "hmm_cov": 1.0, "evalue": 9.816991073061012e-09, "score": 31.086139678955078, "start": 17, "stop": 52 } ], "seq_stats": { "molecular_weight": 5754.7484, "isoelectric_point": 9.428968620300292 }, "id": "DFOKDJOJIG_181", "locus": "GCA013367855_00141" }, { "type": "cds", "sequence": "contig_1", "start": 85209, "stop": 85595, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02729.26" ], "nt": "ATGCCATTAAATTTAAAGGGTCGTAGTTTAGACAGCGCTCTAAACTTTACAACCGAAGAAATTAACTATTTACTAGATTTATCACTAGAACTAAAAAGAGCAAAATTTGCTGGTTTACACACAAATAATCGCCCATTAGTTGGAAAAAACATTGTTATAATGTTCCAAAAAGACTCAACAAGAACTCGTTGTTCATTTGAAGTTGCAGCTGCTGATTTAGGTGCAAGCTGTACATACATTGGCCCAACAGGATCAAACTTTGGTAAAAAAGAATCAGTTATTGACACAGCTAAAGTTTTAGGCCAAATTTATGATGGTATTGAATTCCGTGGATTCAAACAATCAGATGTTGATGCATTAGTAAAATACTCAGGAATTCCTGTATGA", "aa": "MPLNLKGRSLDSALNFTTEEINYLLDLSLELKRAKFAGLHTNNRPLVGKNIVIMFQKDSTRTRCSFEVAAADLGASCTYIGPTGSNFGKKESVIDTAKVLGQIYDGIEFRGFKQSDVDALVKYSGIPV", "aa_hexdigest": "06307b4f1814626b0733c2ae428eb8cc", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BB40E1", "query_cov": 1.0, "subject_cov": 0.8152866242038217, "identity": 0.563, "score": 144.0, "evalue": 8.79e-42, "valid": false, "uniref50_id": "UniRef50_A0A3D1ABF9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02729.26", "name": "OTCace_N", "length": 126, "aa_cov": 0.9375, "hmm_cov": 0.9841269841269841, "evalue": 3.842120641682653e-30, "score": 100.5682373046875, "start": 9, "stop": 128 } ], "seq_stats": { "molecular_weight": 14021.913599999985, "isoelectric_point": 8.562320137023928 }, "id": "DFOKDJOJIG_182", "locus": "GCA013367855_00142" }, { "type": "cds", "sequence": "contig_1", "start": 85861, "stop": 86235, "strand": "+", "frame": 1, "gene": null, "product": "Helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "nt": "ATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGTTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCGAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAGTTTTCCAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGA", "aa": "MNYNIHKYFHLSLEERFFIQRSLENNLSLRAISRQLNRSASTISREIKRNSNFNEYIFLRADKKSKFRAYHKYMFKFNINFEFEEFSKIFKSKYDKKYFGVKATMNYIEENFNVKKPTMRTVFN", "aa_hexdigest": "c57bd1123260a439ee4f69e485682c4a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0AAJ1UWR4", "query_cov": 1.0080645161290323, "subject_cov": 0.8116883116883117, "identity": 0.6, "score": 141.0, "evalue": 1.13e-40, "valid": false, "product": "Helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_A0AAJ1UWR4" }, "pscc": { "uniref50_id": "UniRef50_A0AAJ1UWR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "product": "Helix-turn-helix domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_183", "locus": "GCA013367855_00143" }, { "type": "cds", "sequence": "contig_1", "start": 86419, "stop": 86700, "strand": "+", "frame": 1, "gene": null, "product": "IS30 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "nt": "TTGGTTCTTGGAAAAAGAGCAAATGGCTATAAAAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGCGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGA", "aa": "MVLGKRANGYKNILTLTERKTRVGFATFVSSKSPFEINSKIKELIKNKNLIVKTITIDNGIEFEKIGFLAKSLNIIIYRAEPYASFQRGSNEN", "aa_hexdigest": "151ece11318bfe9f9904904a28af0e1a", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001F20DEBE", "query_cov": 0.978494623655914, "subject_cov": 0.8272727272727273, "identity": 0.67, "score": 125.0, "evalue": 1.07e-35, "valid": false, "product": "IS30 family transposase", "uniref50_id": "UniRef50_UPI001F20DEBE", "go_ids": [ "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F20DEBE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "product": "IS30 family transposase" }, "genes": [], "id": "DFOKDJOJIG_184", "locus": "GCA013367855_00144" }, { "type": "cds", "sequence": "contig_1", "start": 86707, "stop": 86817, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAGAAGAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGA", "aa": "MIRREYQKGFNFNILTQEDLDTTIAKINNMPREILN", "aa_hexdigest": "8ef7b9776e982e4522979ce89f78742f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4340.9349, "isoelectric_point": 6.038881492614746 }, "id": "DFOKDJOJIG_185", "locus": "GCA013367855_00145" }, { "type": "cds", "sequence": "contig_1", "start": 87065, "stop": 87313, "strand": "-", "frame": 3, "gene": "mscL", "product": "Large-conductance mechanosensitive channel", "db_xrefs": [ "COG:COG1970", "COG:M", "KEGG:K03282", "SO:0001217", "UniRef:UniRef50_D1J7K2", "UniRef:UniRef90_D1J7K2" ], "nt": "TTGGGTGCTTTAATTCAATTTATTATTGTTTCAACATTTATTTTTATAGGATTAATGATTGTATTTACAATTAAGAATTCTATTCTATATGCAAAAGCTAAAAAGAACCCAATTGAAGAAGAAATAAAGGAAGAACCTATTCCAACAACAGAAGAATTAATTTTAAATGAATTAAAAAAATTAAATGAAAATTTATTAGAAACGAAAAATTCTGGGAATAAAAAAGATTTAAAAAGCGATTTAAAATAA", "aa": "MGALIQFIIVSTFIFIGLMIVFTIKNSILYAKAKKNPIEEEIKEEPIPTTEELILNELKKLNENLLETKNSGNKKDLKSDLK", "aa_hexdigest": "cc6e49e6d75f21591dde572a2051c64a", "start_type": "TTG", "rbs_motif": null, "genes": [ "mscL" ], "truncated": "3-prime", "pseudogene": { "start": 87065, "stop": 87613, "observations": { "mutation": [ 87367, 87376 ], "stop": [ 87367, 87376 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K2", "query_cov": 1.0, "subject_cov": 0.4293193717277487, "identity": 0.927, "score": 134.0, "evalue": 4.75e-38, "gene_start": 1, "gene_end": 82, "reference_start": 110, "reference_end": 191, "reference_sequence": "FLLIKKTKTRIKFILYNIDMTSKELEEKKHYIKKSYVDAKKIVSRGNMFMLAIGLLLGASFGALVSSLANDVIMSAITKAIGMKNLDAWVVWPGIHATKETGGIFIGKFLGALIQFIIVSTFIFIGLMIVFTIKNSILYAKAKKSPIEEEIKEEPTPTTEELILNELKKLNENLLETKNSGDKKDLKNNLK" }, "paralog": false, "identity": 0.9395604395604396, "score": 330.0, "evalue": 6.99e-118, "description": "Internal stop codon at 87367, 87376. Nonsense mutation around 87367, 87376.", "psc": { "uniref90_id": "UniRef90_D1J7K2", "gene": "MscL", "product": "Large-conductance mechanosensitive channel", "uniref50_id": "UniRef50_D1J7K2", "cog_id": "COG1970", "cog_category": "M", "kegg_orthology_id": "K03282", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K2", "UniRef:UniRef50_D1J7K2", "COG:COG1970", "COG:M", "KEGG:K03282" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7K2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7K2" ], "product": "Large-conductance mechanosensitive channel" } }, "id": "DFOKDJOJIG_186", "locus": "GCA013367855_00146" }, { "type": "cds", "sequence": "contig_1", "start": 87374, "stop": 87583, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01741.23" ], "nt": "ATGACAAGTAAAGAATTAGAAGAAAAAAAGCATTATATTAAAAAGTCATATATAGATGCAAAAAAAATAGTTTCGCGTGGAAACATGTTCATGCTAGCAATTGGGCTTTTATTAGGAGCTTCATTTGGGGCACTAGTATCATCACTAGCAAATGATGTTATTATGTCAGCAATCACAAAGGCAGTCGGTATGAAAAATCTTGATGCTTGA", "aa": "MTSKELEEKKHYIKKSYIDAKKIVSRGNMFMLAIGLLLGASFGALVSSLANDVIMSAITKAVGMKNLDA", "aa_hexdigest": "f66c04029516805ebdcad7631d08d727", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01741.23", "name": "MscL", "length": 47, "aa_cov": 0.6811594202898551, "hmm_cov": 1.0, "evalue": 7.285058772641757e-13, "score": 44.62319564819336, "start": 20, "stop": 66 } ], "seq_stats": { "molecular_weight": 7462.8359999999975, "isoelectric_point": 9.573893547058105 }, "id": "DFOKDJOJIG_187", "locus": "GCA013367855_00147" }, { "type": "tRNA", "sequence": "contig_1", "start": 87646, "stop": 87738, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 68.9, "nt": "GGGTAAGTACTCAAGTGGTCGAAGAGGCGGTCCTGCTAAGACTGTAGGGGTGCTAAAACACCCGCGGAGGTTCAAATCCTCTCTTACCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 87702, 87704 ], "id": "DFOKDJOJIG_33", "locus": "GCA013367855_00148" }, { "type": "cds", "sequence": "contig_1", "start": 87806, "stop": 87973, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTATATTAGTACAAATCCTCAAAGCATTTATTACACAAAGAACATTGAATACTACAAACAATTACAAGAAGCTTCTAATCATGCTAAAAATTTAAAATTAGGAATTTTTGATAGTGGCAATGATATCAATAAAATTTACCCTCAAAGATCAAGGAAAAATTAA", "aa": "MYYISTNPQSIYYTKNIEYYKQLQEASNHAKNLKLGIFDSGNDINKIYPQRSRKN", "aa_hexdigest": "b48206481d340a12df4c8d8f6a79488f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6563.281300000002, "isoelectric_point": 9.476675224304202 }, "id": "DFOKDJOJIG_188", "locus": "GCA013367855_00149" }, { "type": "cds", "sequence": "contig_1", "start": 88130, "stop": 88486, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCGTCAAGAATAAAAAAACTTACTAAGATAGCTAAGCCAAATAATCAAAACGACACAAATAATGACAAATTTAACATCAATAATTTTCATGAAGCTAAATTTGATTTTGAAATACTAAAAAATAATAAATTTGAAGTAGGAAAAGCAACAGAAATAAATATTGCAAGAATTGCTGATGGAGATACAATATCAGATTCCTATCGAAATAAATATCGCTTTAATGGAATTGATACGCCAGAAACTCATAAAATGACTAATGGAAAATTTGAAGAAACGTCTGGCATTCAATATAAATATGGAAAAATTGCCGAAAATTATACAAAGAATTTTCTAAACAATGCTGCTAAAATTTGA", "aa": "MSSRIKKLTKIAKPNNQNDTNNDKFNINNFHEAKFDFEILKNNKFEVGKATEINIARIADGDTISDSYRNKYRFNGIDTPETHKMTNGKFEETSGIQYKYGKIAENYTKNFLNNAAKI", "aa_hexdigest": "21c577a82fcc0e994100a369de678c92", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13619.04889999998, "isoelectric_point": 9.371076011657713 }, "id": "DFOKDJOJIG_189", "locus": "GCA013367855_00150" }, { "type": "cds", "sequence": "contig_1", "start": 88505, "stop": 88759, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAAAAACTAAAAAAATATTGAATCTTGCATTGGGCTCTATTGCTACAATTTTACCAATTTTAATAGTTTCATGTTCTTGCAAACTAGAAAAAATTAATGACGAAGAACAACAAAATTTTAAAGAAAAATATGACAAAAAAATAAAGGAAATTGAAAATAGCAACGAATGGCAAAAAAATGATTATTATAAACAATTAATTGCTGATGCCAAAAAATTAATAAATTCAAACAATAAAAAAATTGATTGA", "aa": "MERKTKKILNLALGSIATILPILIVSCSCKLEKINDEEQQNFKEKYDKKIKEIENSNEWQKNDYYKQLIADAKKLINSNNKKID", "aa_hexdigest": "cd9132459587c1fac86b03099ced3f8e", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9858.32989999999, "isoelectric_point": 9.056534767150879 }, "id": "DFOKDJOJIG_190", "locus": "GCA013367855_00151" }, { "type": "cds", "sequence": "contig_1", "start": 89046, "stop": 89498, "strand": "-", "frame": 2, "gene": "fba", "product": "Fructose-bisphosphate aldolase", "db_xrefs": [ "COG:COG0191", "COG:G", "SO:0001217", "UniRef:UniRef50_A0A3B0NXB7", "UniRef:UniRef90_D1J7K4" ], "nt": "TTGGCAACAAAAGAAGCAAATTTAGATATCATACTCGGAGCTTCTCCTTCTGCAATTAAATACATGGGTGGCTATAAAACCGTTGTCAATTTGGTTAATGGTCTTATTCAAGATTTAGAAATTAAAACTAATGTCATATTGCACTTAGATCATGGTAATTATAACGATTGTATCAATGCTATAAATGCCGGCTTTAAGTCAATAATGTATGATGGTAGCAAAGAAGATTTTGAAACCAATATATTGAACGCAAAAAAAATAGTTAACATTGCAAAAAAATTAGATATAACTGTTGAGGCAGAAGTGGGTAGAATTGGACTAGATATTAGTAAAAATAAATCACTTGATATTCAAAAAACAAACATTGAAGATGCTAAAAAATTAGCAAATACTGGAATTGATTTATTAGCAGTAGCAATTGGAAATATTCATGGTCAATATGAAAGTAATTGA", "aa": "MATKEANLDIILGASPSAIKYMGGYKTVVNLVNGLIQDLEIKTNVILHLDHGNYNDCINAINAGFKSIMYDGSKEDFETNILNAKKIVNIAKKLDITVEAEVGRIGLDISKNKSLDIQKTNIEDAKKLANTGIDLLAVAIGNIHGQYESN", "aa_hexdigest": "33f240cd712925667ad909a3aeb5465a", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "genes": [ "fba" ], "truncated": "both", "pseudogene": { "start": 88743, "stop": 89627, "observations": { "mutation": [ 89048, 89516 ], "stop": [ 89048, 89516 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K4", "query_cov": 1.0, "subject_cov": 0.5102040816326531, "identity": 0.973, "score": 287.0, "evalue": 5.01e-96, "gene_start": 1, "gene_end": 150, "reference_start": 44, "reference_end": 193, "reference_sequence": "MIKLKRKNMEIQNLNQTLKKIKEENYAILHININNLEWAKFVLLATKQANLDIILGASPSAIKYMGGYKTVVNLVNGLIQDLEIKTNVILHLDHGNYNDCINAINAGFKSIMYDGSKEDFETNILNTKKIVNIAKKMDITVEAEVGRIGLDISKNKSLDIQKTNIEDAKKLANTGIDLLAVAIGNIHGQYESNWKGLDFELLDKLNQEIKIPLVLHGASGIKEDDIKKAIRLGIKKINFNTELQIANAKALNDFLKNNNFLENKNYNPRILYANSNQAIYKCTLDLLNKYGKKN" }, "paralog": false, "identity": 0.9829931972789115, "score": 566.0, "evalue": 2.61e-208, "description": "Internal stop codon at 89048, 89516. Nonsense mutation around 89048, 89516.", "psc": { "uniref90_id": "UniRef90_D1J7K4", "gene": "Fba", "product": "Fructose-bisphosphate aldolase", "uniref50_id": "UniRef50_A0A3B0NXB7", "cog_id": "COG0191", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K4", "UniRef:UniRef50_A0A3B0NXB7", "COG:COG0191", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NXB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NXB7" ], "product": "Fructose-bisphosphate aldolase" } }, "id": "DFOKDJOJIG_191", "locus": "GCA013367855_00152" }, { "type": "cds", "sequence": "contig_1", "start": 89825, "stop": 89935, "strand": "-", "frame": 3, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "EC:2.7.7.6", "GO:0003899", "GO:0006351", "SO:0001217", "UniRef:UniRef50_N9VBR1", "UniRef:UniRef90_D1J7K5" ], "nt": "ATGGAATATAAATCGCAAGTTGATATAGCTAAAGAAATTTTAGATAGACACGATGCACTAGATTTTGAAACTTTATTTAATTATGTAAAAGACGAACTACTTGAACGTTGA", "aa": "MEYKSQVDIAKEILDRHDALDFETLFNYVKDELLER", "aa_hexdigest": "c8d54175b4f84f2b8947c9e2089e7878", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 89678, "stop": 89935, "observations": { "mutation": [ 89710, 89827 ], "stop": [ 89710, 89827 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K5", "query_cov": 1.0, "subject_cov": 0.4235294117647059, "identity": 1.0, "score": 72.8, "evalue": 5.06e-16, "gene_start": 1, "gene_end": 36, "reference_start": 1, "reference_end": 36, "reference_sequence": "MEYKSQVDIAKEILDRHDALDFETLFNYVKDELLERWKEELTSSISDEELLIKKRGELYKLLTIDGRFFKKVNGLWTSKRPEFKN" }, "paralog": false, "identity": 0.9764705882352941, "score": 162.0, "evalue": 9.57e-54, "description": "Internal stop codon at 89710, 89827. Nonsense mutation around 89710, 89827.", "psc": { "uniref90_id": "UniRef90_D1J7K5", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_N9VBR1", "go_ids": [ "GO:0003899", "GO:0006351" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K5", "EC:2.7.7.6", "UniRef:UniRef50_N9VBR1", "GO:0003899", "GO:0006351" ] }, "pscc": { "uniref50_id": "UniRef50_N9VBR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VBR1" ], "product": "HTH HARE-type domain-containing protein" } }, "id": "DFOKDJOJIG_192", "locus": "GCA013367855_00153" }, { "type": "cds", "sequence": "contig_1", "start": 90387, "stop": 90989, "strand": "+", "frame": 3, "gene": null, "product": "arginine deiminase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005698E2F" ], "nt": "ATGTCTGTATTTGACAGTAAATTTAATGGCATTCACGTTTATTCAGAAATCGGAGAACTAGAAACTGTTCTAGTTCACGAACCTGGACGTGAAATTGACTACATTACACCAGCTCGTTTAGACGAATTACTATTCTCAGCTATTCTAGAAAGCCACGATGCTAGAAAAGAACACCAATCATTTGTTAAAATAATGAAGGACAGAGGAATCAACGTTGTTGAATTAACTGACCTAGTTGCTGAAACATATGATTTAGCATCAAAAGCAGCTAAAGAAGAATTCATTGAAACATTCTTGGAAGAAACAGTTCCTGTTTTAACAGAAGCAAACAAAAAAGCTGTAAGAGCATTCTTATTAAGCAAACCAACTCATGAAATGGTTGAATTCATGATGTCAGGTATTACTAAATATGAATTAGGAGTTGAATCAGAAAACGAACTAATCGTTGACCCAATGCCTAACTTATACTTTACACGTGACCCATTTGCATCAGTAGGTAATGGTGTTACAATTCACTTTATGCGTTACATCGTAAGACGCCGTGAAACACTATTTGCAAGATTTGTATTTAGAAATCACCCTAAACTAGTTAAAACTCCATGA", "aa": "MSVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTP", "aa_hexdigest": "a1b2024decd2b61298954f3300560145", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0005698E2F", "query_cov": 0.915, "subject_cov": 0.9104477611940298, "identity": 0.574, "score": 216.0, "evalue": 3.11e-68, "valid": false, "product": "arginine deiminase family protein", "uniref50_id": "UniRef50_UPI0005698E2F" }, "pscc": { "uniref50_id": "UniRef50_UPI0005698E2F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005698E2F" ], "product": "arginine deiminase family protein" }, "genes": [], "id": "DFOKDJOJIG_193", "locus": "GCA013367855_00154" }, { "type": "cds", "sequence": "contig_1", "start": 91005, "stop": 91175, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGTCAATTGAAGGTGGTGACGTATTTATCTACAACAACGAAACATTAGTTGTTGGTGTTTCAGAAAGAACTGACCTAGATACTATTACTTTATTAGCTAAAAACATCAAGGCAAACAAAGACGTTGAATTTAAACGTATTGTTGCAATTAATGTTCCTAAATGA", "aa": "MKMSIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKDVEFKRIVAINVPK", "aa_hexdigest": "756abf8800f3830929735dd4d49184ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6252.2179000000015, "isoelectric_point": 6.125447273254394 }, "id": "DFOKDJOJIG_194", "locus": "GCA013367855_00155" }, { "type": "cds", "sequence": "contig_1", "start": 91320, "stop": 91613, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCACTAGACAAATTATTAGCTTCAATCATTAACAAAGAACCAGTTTTAATTCCTATTGGTGGTGCTGGAGCAACTGAAATGGAAATTGCTCGTGAAACAAACTTCGATGGAACCAACTACCTAGCAATTAAACCAGGACTTGTTATTGGTTATGACCGTAACGAAAAAACAAATGCTGCTTTAAAAGCTGCTGGTATTACTGTATTACCATTCCACGGAAACCAATTATCTCTAGGTATGGGAAATGCACGTTGCATGTCAATGCCTCTATCACGTAAGGATGTTAAGTGA", "aa": "MPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVK", "aa_hexdigest": "7c5eac652468c5e656c36d6582d9a4e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10354.017699999995, "isoelectric_point": 8.851718711853028 }, "id": "DFOKDJOJIG_195", "locus": "GCA013367855_00156" }, { "type": "cds", "sequence": "contig_1", "start": 91734, "stop": 92030, "strand": "-", "frame": 2, "gene": "dut2", "product": "dUTP diphosphatase", "db_xrefs": [ "COG:COG4508", "COG:F", "SO:0001217", "UniRef:UniRef50_D1J7K7", "UniRef:UniRef90_D1J7K7" ], "nt": "ATGTTAGAAGAATATGCGGATGGAATTCATTTTATCACTAGCATTGCGCTAAAAAATAACATAACCTCATTTGATATAAAGCCAATAATTAAATATAATGACTTTACCTTGCAACTTGCCTATACATATCAATGTTTTACTAAGTTGTTTTTGCACCATAATAAAAGATTAATAAAAAAAGCATACGGTGTTTATTTAGGACTAGGCGAAATTATGAATATTGATCGCGATATGATTTGCACGGCCTATGCCAAAAAAAATCATAAAAACTATGAAAGAATAAAAAATAATTATTAA", "aa": "MLEEYADGIHFITSIALKNNITSFDIKPIIKYNDFTLQLAYTYQCFTKLFLHHNKRLIKKAYGVYLGLGEIMNIDRDMICTAYAKKNHKNYERIKNNY", "aa_hexdigest": "9131402a92497820c79d2cde424a1510", "start_type": "ATG", "rbs_motif": null, "genes": [ "dut2" ], "truncated": "3-prime", "pseudogene": { "start": 91734, "stop": 92261, "observations": { "mutation": [ 92063 ], "stop": [ 92063 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K7", "query_cov": 1.0, "subject_cov": 0.56, "identity": 1.0, "score": 204.0, "evalue": 6.06e-66, "gene_start": 1, "gene_end": 98, "reference_start": 78, "reference_end": 175, "reference_sequence": "MELFLTFKFIIQNMNLTEVFEAQKKLDLAFASSIEKTEFLNIKIKMVIALLVELGEFANEVKLFKYWKKDKSINQISMLEEYADGIHFITSIALKNNITSFDIKPIIKYNDFTLQLAYTYQCFTKLFLHHNKRLIKKAYGVYLGLGEIMNIDRDMICTAYAKKNHKNYERIKNNY" }, "paralog": false, "identity": 0.9885714285714285, "score": 344.0, "evalue": 1.71e-123, "description": "Internal stop codon at 92063. Nonsense mutation around 92063.", "psc": { "uniref90_id": "UniRef90_D1J7K7", "gene": "Dut2", "product": "dUTP diphosphatase", "uniref50_id": "UniRef50_D1J7K7", "cog_id": "COG4508", "cog_category": "F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K7", "UniRef:UniRef50_D1J7K7", "COG:COG4508", "COG:F" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7K7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7K7" ], "product": "dUTP diphosphatase" } }, "id": "DFOKDJOJIG_196", "locus": "GCA013367855_00157" }, { "type": "cds", "sequence": "contig_1", "start": 92061, "stop": 92261, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08761.16" ], "nt": "ATGGAATTATTTTTAACTTTTAAGTTTATAATTCAAAACATGAATTTAACAGAAGTTTTTGAAGCCCAAAAGAAATTAGACCTTGCATTTGCAAATAGTATTGAAAAAACAGAATTTTTGAATATCAAAATCAAAATGGTTATTGCTTTGCTTGTTGAACTAGGAGAATTTGCAAACGAAGTAAAACTATTTAAATATTGA", "aa": "MELFLTFKFIIQNMNLTEVFEAQKKLDLAFANSIEKTEFLNIKIKMVIALLVELGEFANEVKLFKY", "aa_hexdigest": "616d1077d09d512230a2b21d98a99e41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08761.16", "name": "dUTPase_2", "length": 52, "aa_cov": 0.7878787878787878, "hmm_cov": 1.0, "evalue": 3.5242113846672005e-10, "score": 36.042789459228516, "start": 15, "stop": 66 } ], "seq_stats": { "molecular_weight": 7762.191499999994, "isoelectric_point": 5.319015693664551 }, "id": "DFOKDJOJIG_197", "locus": "GCA013367855_00158" }, { "type": "tRNA", "sequence": "contig_1", "start": 92297, "stop": 92380, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 69.5, "nt": "GCCCCGATGGCGGAATGGTAGACGCAGTTGACTCAAAATCAACCGGTGAGAGCCATGCTGGTTCAAGTCCAGTTCGGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 92330, 92332 ], "id": "DFOKDJOJIG_3", "locus": "GCA013367855_00159" }, { "type": "cds", "sequence": "contig_1", "start": 92617, "stop": 93354, "strand": "+", "frame": 1, "gene": null, "product": "RDD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TR13", "UniRef:UniRef90_D1J7K8" ], "nt": "TTGCTAATATCTGCAATTGGTTTGCTTGTTTTGATTTATATTACATTGGTCTTTAAAAACAAATATGAAGAATCATTTGAACTAGAAATTTTTAATGATATTTTTGTAAGTTGTTTAGTAGGAATATTTTCAGGAGCATTATTAGTCTTGGCTTCCTTTATATTACTAGATGTTTTTAAAAGAAATAAAATCAAAAATTTCTTTGAATATTATTGTTATCTTTCTTCATTAAAAAGTAAAAATAAATTTTTATTTTTTAAAAATTATGATTTAAGAGAAATAATAAATAATAAAAATCAACTAACCAAACAAGAATTTATTTGCTTTGTTGCCGAAAAATTAAACTATACAATGCCTTCTATTGAATATAAAAACTTAGTTAATGAAATTAACAGTGATTTTGCCAAACATTCATTTTTAGATCCGGATTTTAAAAAGCAAAAGAAAGCAACACTTACTAGGTTAATTATCTTTGAAATTATCATTCCTTTATTTATTTCTATTGCATTGATAGTATTAATTGCATGTCTTTTTAATAATGATGATCAACCAATCAGGGCAATTTATAGGTTAATAATTATTTTAGTCAGTGCAATAATTGTTTTAAGCCTATCGATTTTAATTTATGAACTATTTATTTTAAGTAAAGTAAAAAATTATCAAAATTTCAATAATTTATATTTACTTTCATTTAATAATTACTCATATAAGTATTTAAATTCTAGTTTAGTAAGATAG", "aa": "MLISAIGLLVLIYITLVFKNKYEESFELEIFNDIFVSCLVGIFSGALLVLASFILLDVFKRNKIKNFFEYYCYLSSLKSKNKFLFFKNYDLREIINNKNQLTKQEFICFVAEKLNYTMPSIEYKNLVNEINSDFAKHSFLDPDFKKQKKATLTRLIIFEIIIPLFISIALIVLIACLFNNDDQPIRAIYRLIIILVSAIIVLSLSILIYELFILSKVKNYQNFNNLYLLSFNNYSYKYLNSSLVR", "aa_hexdigest": "bc1e90a0caa3705d870abcc76274be7a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7K8", "query_cov": 1.0, "subject_cov": 0.9074074074074074, "identity": 0.996, "score": 446.0, "evalue": 1.38e-157, "valid": true, "uniref50_id": "UniRef50_N9TR13" }, "pscc": { "uniref50_id": "UniRef50_N9TR13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TR13" ], "product": "RDD domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_198", "locus": "GCA013367855_00160" }, { "type": "cds", "sequence": "contig_1", "start": 93717, "stop": 93938, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGATAAACAAAATTCTTTTAGGGCTAGCACCTGCTGCAGCACTTATTGCTGCATCAGGACTTTCTGCTGCTTGTGGTCATACAGGTGGTGGTTCAGGAACTAGCTATGGATTTGACCAAACAGATGATGGAAAATTAGTATTAGCCTCAGGATTTTCTAACACAAATCCACAAGGCATTGCCTTAAAAGCAGTTGTTGATGCATACAACAAATGA", "aa": "MKKINKILLGLAPAAALIAASGLSAACGHTGGGSGTSYGFDQTDDGKLVLASGFSNTNPQGIALKAVVDAYNK", "aa_hexdigest": "4d5d46dbd68f8a37a66a009cb275a098", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7209.132800000001, "isoelectric_point": 8.890399742126466 }, "id": "DFOKDJOJIG_199", "locus": "GCA013367855_00161" }, { "type": "cds", "sequence": "contig_1", "start": 93993, "stop": 94217, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAATGGATATAATACTAACACTTTAAGAAGTGACTTGGATGCTAAAAACTCAAATGACTTTTATAACATGACATTAAATTATCCAGCTGCTGCTTCGATATTGTCTCAATACAAAATGAATTTAGCTATTTCAAAAGAAGATTATGATAAATTCGGTATTGAAAATGGATTTAATTTAGGTAATGATTTAATTGGTGGAAACACTAAAAAAGAAAAATGA", "aa": "MANGYNTNTLRSDLDAKNSNDFYNMTLNYPAAASILSQYKMNLAISKEDYDKFGIENGFNLGNDLIGGNTKKEK", "aa_hexdigest": "38631733507daf8f03dc1e3ab2f14276", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8248.035399999997, "isoelectric_point": 5.125819969177246 }, "id": "DFOKDJOJIG_200", "locus": "GCA013367855_00162" }, { "type": "cds", "sequence": "contig_1", "start": 94245, "stop": 94400, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAGTTGCTAAAGCATTAGTAGGAAAATTCATTAAAGAATTAAAAGACTTGGGTGTAAAAATTTCTGAAAAAGACAATACTAAGGTTAACGAATATCTAAAATATTACACAGACAATACTAATGAAGCAAAAGGTGTTGATGAAACTTGA", "aa": "MLSVAKALVGKFIKELKDLGVKISEKDNTKVNEYLKYYTDNTNEAKGVDET", "aa_hexdigest": "4a1fbd4775b77999e512ae18d5585405", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5752.501400000003, "isoelectric_point": 6.161596870422362 }, "id": "DFOKDJOJIG_201", "locus": "GCA013367855_00163" }, { "type": "cds", "sequence": "contig_1", "start": 94464, "stop": 94775, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATCAAATCTTTAGAGATTATTCAAGTATGATTAATTTCTCAATTCAAGCAAAGAAAATGTACAACAAAGATCCTTTATTAAATGTTATTGGTTTTGACTCACTACCAAATGTTATTAATACAATGACAACTTCATTAACTGGTGGAAATATTAATAAAGGATATATAAAACCAGATGAAGATAAAATAACAACAGGCGGATTTGACTACGCAACATTTCTAAAAGATGCAAATAGTGAACAATCAAAATTATTCAAAAAGATGGTTGACTTAATTTTAGAAGGTGTTAAGGAAGGCGCTGTTTGA", "aa": "MSDQIFRDYSSMINFSIQAKKMYNKDPLLNVIGFDSLPNVINTMTTSLTGGNINKGYIKPDEDKITTGGFDYATFLKDANSEQSKLFKKMVDLILEGVKEGAV", "aa_hexdigest": "47e46d26e63b4f47e4b93a6c2f2e8eb9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11481.01039999999, "isoelectric_point": 5.286901664733886 }, "id": "DFOKDJOJIG_202", "locus": "GCA013367855_00164" }, { "type": "cds", "sequence": "contig_1", "start": 94830, "stop": 94988, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCTTGGTTCAACTGCTGGATACGAACATACATTTTTAAAAGATGATAAAGAAAATCCATTAACAAACTTAAAAATAAAAGACACAACTACTTTATTAGATAATCAATCTACATTAGAAGTTTCTAACAAAAAACCTAATGCATCAGATAAATGA", "aa": "MSLGSTAGYEHTFLKDDKENPLTNLKIKDTTTLLDNQSTLEVSNKKPNASDK", "aa_hexdigest": "24b63391f4ba877653ea1b7bcd490927", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5753.319300000003, "isoelectric_point": 5.608667182922363 }, "id": "DFOKDJOJIG_203", "locus": "GCA013367855_00165" }, { "type": "cds", "sequence": "contig_1", "start": 95451, "stop": 95843, "strand": "+", "frame": 3, "gene": null, "product": "Putative mycoplasma lipoprotein, C-terminal region", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215C1230", "UniRef:UniRef90_UPI00215C1230" ], "nt": "ATGTTCCAACATGATTTAGAAAACTTTAAAATTGAAAAAGTTTCTAAAGGAAAAACTATAATTACAACATTTGACGGCAAGCAAAAACCTATTGATATTTTCAATGAATACGGAAGCTATATTTCACCAACTAAGTCATACTTCTCATCAGATAAAGCAAGCAAATTAAATAAAGCAACAAAAATAGCATTTGACAACTTTAAACAACTTGACAATAAAGATAGTGGTTATATTCCATCAGAAGATATTGCAACTTCATTATCAGATGTTTTAAGAGAAGCAATAACAAGTGCTGGAAAAGCAGCATTATCAAAAGCAAACGCTAATAACGAAGTATTTGAATATAAGGAATTTGTTTCAAAAATTTTAGGTCAATTAAGTAACCAACTTTAA", "aa": "MFQHDLENFKIEKVSKGKTIITTFDGKQKPIDIFNEYGSYISPTKSYFSSDKASKLNKATKIAFDNFKQLDNKDSGYIPSEDIATSLSDVLREAITSAGKAALSKANANNEVFEYKEFVSKILGQLSNQL", "aa_hexdigest": "95a626570b862e12f446a9329ea061b3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 95283, "stop": 95843, "observations": { "mutation": [ 95325, 95448 ], "stop": [ 95325, 95448 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215C1230", "query_cov": 1.0, "subject_cov": 0.7027027027027027, "identity": 0.9229999999999999, "score": 227.0, "evalue": 4.63e-74, "gene_start": 1, "gene_end": 130, "reference_start": 57, "reference_end": 185, "reference_sequence": "MVSSNKILNEADADWISSPLAFDKNAKKAVFIQGPSILPIHANEKEDKATKLFINWMFQHDLENFKIEEVFKGKTTTKTFEGKQKPIDIFNEYGSYISPTKSYFSSDKASKLNKATKIAFDNFKQLDKEDSGYIPSEDIATSLSDVLREAITSAGKAALSKANVNEVFEYKEFVSKILGQLSNQL" }, "paralog": false, "identity": 0.9139784946236559, "score": 325.0, "evalue": 3.17e-115, "description": "Internal stop codon at 95325, 95448. Nonsense mutation around 95325, 95448.", "psc": { "uniref90_id": "UniRef90_UPI00215C1230", "product": "Putative mycoplasma lipoprotein, C-terminal region", "uniref50_id": "UniRef50_UPI00215C1230", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215C1230", "UniRef:UniRef50_UPI00215C1230" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215C1230", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215C1230" ] } }, "id": "DFOKDJOJIG_204", "locus": "GCA013367855_00166" }, { "type": "cds", "sequence": "contig_1", "start": 95931, "stop": 96470, "strand": "+", "frame": 3, "gene": "yncB", "product": "TNase-like domain-containing protein", "db_xrefs": [ "COG:COG1525", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J7L0", "UniRef:UniRef90_D1J7L0" ], "nt": "ATGAAAAAATACAATAAATTTTTATTAATTTTAGCCTCATCTGCTGGCTTAGTGGTCGGGGCTCCGTTACTGTCAGTATCATGCAAGACAGTGAATAAAGAAAAAATAAATTTATCAAAATTTGATGTAATAAAGAAAAATTATGAAATTCCAGTATATGAATTTTCAAAAGTGGGAACAATTTTTAATAAATCAATAAATTATAGAATGTATGTCACTGATGAAAAAGGAAATAAAACTATTAAAGAAGTTTCTCAATCATTATGGGATTATTATAATTATATTGAAGGCGAAGTTACTAGAATTTTTGATGGCGACACCATTGAAATAAAAGTAACAAAGCAACCAAATCCTAAACCTGGTGGTAAAGTTCTTAATGTTCCTACAACAATGAAATTAAGAATTCCAATGATTGATACATTAGAAGAAAATACGCCAGAAGTTGGAAAAGAAGAAAAGGAATTAGCAAAAAAAGATCATGCATATGCCGAAAAATTAATTCCTATTGGTTCAAAAGTTAGAGTAATTTCAAGCAATTGA", "aa": "MKKYNKFLLILASSAGLVVGAPLLSVSCKTVNKEKINLSKFDVIKKNYEIPVYEFSKVGTIFNKSINYRMYVTDEKGNKTIKEVSQSLWDYYNYIEGEVTRIFDGDTIEIKVTKQPNPKPGGKVLNVPTTMKLRIPMIDTLEENTPEVGKEEKELAKKDHAYAEKLIPIGSKVRVISSN", "aa_hexdigest": "f2c2cb03f8b584a203ee9810c5b3a221", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "yncB" ], "truncated": "3-prime", "pseudogene": { "start": 95931, "stop": 96773, "observations": { "mutation": [ 96468 ], "stop": [ 96468 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L0", "query_cov": 1.0, "subject_cov": 0.5559006211180124, "identity": 0.9890000000000001, "score": 346.0, "evalue": 3.24e-118, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MKKYNKFLLILASSAGLVVGAPLLSVSCKTVDKEKINLSKFDVIKKNYEIPVYEFSKVGTIFNKSINYRMYVTDEKGDKTIKEVSQSLWDYYNYIEGEVTRIFDGDTIEIKVTKQPNPKPGGKVLNVPTTMKLRIPMIDTLEENTPEVGKEEKELAKKDHAYAEKLIPIGSKVRVISSNWSEKTYDRFVGYVFFGKNFERQFDIEMLAGGYTLARLDTTKVISEFIADLSKESTEAKEIRSYLLPYAAYAINYGILNRKGFYGEVANMRSPYELSSKYLEHGQGMVDFSLSILHYNYWKKPKLATKKNNIYRYLEQINQNKK" }, "paralog": false, "identity": 0.9892857142857143, "score": 548.0, "evalue": 2.31e-200, "description": "Internal stop codon at 96468. Nonsense mutation around 96468.", "psc": { "uniref90_id": "UniRef90_D1J7L0", "gene": "YncB", "product": "TNase-like domain-containing protein", "uniref50_id": "UniRef50_D1J7L0", "cog_id": "COG1525", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L0", "UniRef:UniRef50_D1J7L0", "COG:COG1525", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L0" ], "product": "TNase-like domain-containing protein" } }, "id": "DFOKDJOJIG_205", "locus": "GCA013367855_00167" }, { "type": "cds", "sequence": "contig_1", "start": 96546, "stop": 96824, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCAGGTGGATATACTCTTGCTAGATTAGATACAACAAAAGTTATTTCAGAATTTATTGCTGATTTATCAAAAGAAAGCACTGAAGCAAAGGAAATAAGAAGTTACTTGTTGCCTTATGCTGCATATGCAATTAATTATGGAATTTTAAATAGAAAAGGATTTTATGGTGAAGTTGCCAACATGCGTTCTCCATATGAACTAAGTTCAAAATATTTGGAACACGGACAAGGCATGGTTGATTTCTCACTTTCAATACTTCATTATAATTATTGA", "aa": "MLAGGYTLARLDTTKVISEFIADLSKESTEAKEIRSYLLPYAAYAINYGILNRKGFYGEVANMRSPYELSSKYLEHGQGMVDFSLSILHYNY", "aa_hexdigest": "8df4e3bfd313c4224aaa41d011cbb95d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10429.735599999998, "isoelectric_point": 6.0617876052856445 }, "id": "DFOKDJOJIG_206", "locus": "GCA013367855_00168" }, { "type": "cds", "sequence": "contig_1", "start": 96911, "stop": 97309, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAAAGAAACAAAATCAAGAGAAGAATTATTAAAGGATATTCATAGTTTTTCTAAGCAACGTCATGAAATTAAAAAGACATTGCCTGCAATTGAAATTAAAAATTTAGTTATTGACTTTGGTGAATCACTAGCAGTCGATAATGCTTCATTTTGCATTAATAAAGGAGAATTAGTAACATTATTAGGACCATCTGGTTCTGGTAAAACAACTACCTTGAATGCCATTGCGGGACTTTTAAGACCAACAGGGGGAAAAATTTTCTTTTCAGGAATTGATGTAACAAAATTCTCACCTCAACAAAGAGAATTAGGATTGGTGTTCCAAAACTATGCACTATATCCTCACATGTCAGTTTACGAAAATATTGCATTTCCTCTATACAATGACAAACATTGA", "aa": "MKETKSREELLKDIHSFSKQRHEIKKTLPAIEIKNLVIDFGESLAVDNASFCINKGELVTLLGPSGSGKTTTLNAIAGLLRPTGGKIFFSGIDVTKFSPQQRELGLVFQNYALYPHMSVYENIAFPLYNDKH", "aa_hexdigest": "8d40a7fa5d1c53b48bb03bab9d1f50ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 78, "aa_cov": 0.5757575757575758, "hmm_cov": 1.0, "evalue": 2.960839263514467e-21, "score": 72.1390151977539, "start": 47, "stop": 122 } ], "seq_stats": { "molecular_weight": 14727.77869999998, "isoelectric_point": 8.66733913421631 }, "id": "DFOKDJOJIG_207", "locus": "GCA013367855_00169" }, { "type": "cds", "sequence": "contig_1", "start": 97346, "stop": 98320, "strand": "+", "frame": 2, "gene": "potA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG3842", "COG:E", "SO:0001217", "UniRef:UniRef50_D1J7L1", "UniRef:UniRef90_D1J7L1" ], "nt": "TTGAAAATTTTTGAAATCATTTTAAATGCTTATAAAGTTGATGAAAAAGCAATTGAAGATTTAAAGACAAAATGCTACTATTCAATTGACAACCCTAAAGAAACTAAGAAGTATTTAAATGAATTAATTTCACAATATAACGATATTATTGATGAACAAATTAACAAATTAAACTTTTATAAAACTAAAAAAACAGCCGAAGGAACTTCGCTAACTAAAAATGTTTTGCAATATATTAAAAAACTAGGATTGCAACGCCAAGCAGAAATTTCAGAAGCTAGAGAAAAATTTTTGGGTGAAAAACAAAAACTTGATAGAGAATTTGAATTTACTCATGACAAAAATATTTTAAAAAATATATCAGCATTAAAATCAGAATTTTCAGATACTGTTTCTAAAATTAAGTCTAAAAGTGCAGAAGAGATTGCTAATGCTAAAAAAGAATATTCTGAAAAATATAACGAAGCGTTAGCTCGTAATACTCAAGCAATTAAAGATGCAAAACTAGAACTAATGGAAAAGAAACACGAACAAGCAGAATCTCCATTAGTTAATAAAATAAAAGAAATAAAGAATAGATACGTTTTAGTTTCAAAAATAACTAATGTTGAATATACAGAAGCAGTTGAAAATGTTTTTGGTTCGTTGTTCAATTCTCAAAATATAACAAAAGGTAATTTTGATACTAAACTTAAGGCTTTAATATTTGCTTTAGATCAAGAAACTCAAAATCAAATTTCAGAAATTTCTAAAGATGTTTTGACAATTGATGAAGCAATAGTAAAAGATGTATTAGATGTAGCACAAAGAGTTGAAATTACAAAGAACCTAGCAAAACGTCCTACTCAAATGTCCGGTGGTCAACAACAACGTGTTGCTATAGCTAGAGCAATAGTTAAAAAACCAAAAATATTATTATTGGATGAACCATTGTCAAACTTGGATGCTAAGTTGCGTATTTCAACAAGAAAGTGA", "aa": "MKIFEIILNAYKVDEKAIEDLKTKCYYSIDNPKETKKYLNELISQYNDIIDEQINKLNFYKTKKTAEGTSLTKNVLQYIKKLGLQRQAEISEAREKFLGEKQKLDREFEFTHDKNILKNISALKSEFSDTVSKIKSKSAEEIANAKKEYSEKYNEALARNTQAIKDAKLELMEKKHEQAESPLVNKIKEIKNRYVLVSKITNVEYTEAVENVFGSLFNSQNITKGNFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRK", "aa_hexdigest": "6a9aa395f3745b512025ad300a58e48f", "start_type": "TTG", "rbs_motif": null, "genes": [ "potA" ], "truncated": "both", "pseudogene": { "start": 97046, "stop": 98623, "observations": { "mutation": [ 97307, 98318 ], "stop": [ 97307, 98318 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L1", "query_cov": 1.0, "subject_cov": 0.500772797527048, "identity": 0.985, "score": 513.0, "evalue": 4.85e-177, "gene_start": 1, "gene_end": 324, "reference_start": 146, "reference_end": 469, "reference_sequence": "MKETKSREELLKDIHSFSKQRHEIKKTLPAIEIKNLVIDFGESLAVDNASFCINKGELVTLLGPSGSGKTTTLNAIAGLLRPTGGKIFFSGIDVTKFSPQQRELGLVFQNYALYPHMSVYENIAFPLYNDKHWREETLEKTKLSQLKIFEIILNAYKVDEKAIEDLKTKCYYSIDNPKETKKYLNELISQYNDIIDEQINKLNFYKTKKTAEGTSLTKNVLQYIKKLGLQRQAEISEAREKFLGEKQKLDREFEFTHDKNILKNISVLKSEFSDTVSKIKSKSAEEIANVKKEYSEKYNEALARNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRYVLVSKITNVEYTEAVENVFGSLFNSQNITKGNFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRKWIRSIQQELGITTVFVTHDQEEAMSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKSSIDVGIRGESLIEEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV" }, "paralog": false, "identity": 0.9885714285714285, "score": 921.0, "evalue": 0.0, "description": "Internal stop codon at 97307, 98318. Nonsense mutation around 97307, 98318.", "psc": { "uniref90_id": "UniRef90_D1J7L1", "gene": "PotA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_D1J7L1", "cog_id": "COG3842", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L1", "UniRef:UniRef50_D1J7L1", "COG:COG3842", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7L1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L1" ], "product": "ABC transporter ATP-binding protein" } }, "id": "DFOKDJOJIG_208", "locus": "GCA013367855_00170" }, { "type": "cds", "sequence": "contig_1", "start": 98387, "stop": 98851, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGF6", "UniRef:UniRef90_UPI00215C93E5" ], "nt": "ATGTCAATTAGCGATAAAATCGTATGTATGTCAACTGCTAAAATTCAACAAATTGGTTCTCCTATGGAACTATATTTGAAACCTAAAAATGAATTTGTTGCCAAATTCTTAGGTATGCCAGAAATGACAATATTTGAAGCACAAATTAAAGACGGATATATAATTTACAACAATAAAAAACTACTTAAAGCTCCTGAAGGATATCAAAAATCTTCTATTGATGTTGGTATAAGAGGCGAATCTCTAATAGAAGAAGAAGAAGGAATATTTGAAGGAAGTATAAAATTAGTTGAACATTTAGGCAAGGAAATTCAAGCTCAAATATTTATCAAAGAATTAAATAAAATTGCAAACGTTTTCTTAGCTAAAAAACACAATTACGAATTAGGTGAAGTTGTTAAGTTAAATGCAAAAAGTGAAGGCTTATTCCATTTATTTGATGTAGACAGCAAAGAACGTGTATAA", "aa": "MSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKSSIDVGIRGESLIEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV", "aa_hexdigest": "357f93e0ad966fff840a283822b7949b", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 98087, "stop": 98851, "observations": { "mutation": [ 98318 ], "stop": [ 98318 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215C93E5", "query_cov": 1.0, "subject_cov": 0.42896935933147634, "identity": 1.0, "score": 299.0, "evalue": 1.35e-99, "gene_start": 1, "gene_end": 154, "reference_start": 206, "reference_end": 359, "reference_sequence": "ANAKKEYSEKYNEALDRNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRCVLVSKITNVEYTEAVENVFGSLFNSQNITKGSFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRKWIRSIQQELGITTVFVTHDQEEAMSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKSSIDVGIRGESLIEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV" }, "paralog": false, "identity": 0.9960629921259843, "score": 483.0, "evalue": 1.23e-174, "description": "Internal stop codon at 98318. Nonsense mutation around 98318.", "psc": { "uniref90_id": "UniRef90_UPI00215C93E5", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3G8LGF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215C93E5", "UniRef:UniRef50_A0A3G8LGF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LGF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGF6" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "DFOKDJOJIG_209", "locus": "GCA013367855_00171" }, { "type": "cds", "sequence": "contig_1", "start": 98950, "stop": 99225, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCATCATTAATTACATTAACTTTATTTACTATAATTCCATTTATTTTGGTTTTAATCTTTTCATCAACATATCAAGTTGGTGCAACGGTTTATGATAAGGGATTTGGACTAAGAAACTATATTAAGTTGTCAAAAGATCCAATATTCCACATCGCTATTAGAAATACATTGGTTTATGCTATATTAGCTTTACCAATTAGCTTGGCGATTTCCATTTTAATTTCATCTGCAATAAGCTTTGTAATCAAACAAAGTTTCCGTAGATTTTGA", "aa": "MLPSLITLTLFTIIPFILVLIFSSTYQVGATVYDKGFGLRNYIKLSKDPIFHIAIRNTLVYAILALPISLAISILISSAISFVIKQSFRRF", "aa_hexdigest": "e30282d6899a0424603a264d62d03497", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10209.208099999989, "isoelectric_point": 10.207359886169431 }, "id": "DFOKDJOJIG_210", "locus": "GCA013367855_00172" }, { "type": "cds", "sequence": "contig_1", "start": 99129, "stop": 99320, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATAAATCCGCCTGATGTTCTAAATAAGAATGCAAAGGCGATTGAAACAGCAATTCCTGATGTTACATATGGTAGAAAGAATACTGTTTGTCAAAATCTACGGAAACTTTGTTTGATTACAAAGCTTATTGCAGATGAAATTAAAATGGAAATCGCCAAGCTAATTGGTAAAGCTAATATAGCATAA", "aa": "MLINPPDVLNKNAKAIETAIPDVTYGRKNTVCQNLRKLCLITKLIADEIKMEIAKLIGKANIA", "aa_hexdigest": "40516630ad587404f4a99841c8dc8d2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6948.288799999998, "isoelectric_point": 9.342967796325684 }, "id": "DFOKDJOJIG_211", "locus": "GCA013367855_00173" }, { "type": "cds", "sequence": "contig_1", "start": 99448, "stop": 99675, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATCAGTTAACCCTGATTTATATAAATCAGCCTCTATTGATGGAAGTTCAAATTTAAAACAATTTTTTGCAATTACTTTGCCATCAATTAGAAAAACAATTACTTTCCTATTTACAGTAGGAATTATTGGATCAATTAAAACATTCCCATTGGCTTTATTTGATAACAAACCTGCTGAAGCAGCTGCAAACTCTGGTATGAGTATCATGCTATATATATTCTGA", "aa": "MLSVNPDLYKSASIDGSSNLKQFFAITLPSIRKTITFLFTVGIIGSIKTFPLALFDNKPAEAAANSGMSIMLYIF", "aa_hexdigest": "50ec91ad7d2108c5968bc822baf7c8d8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8149.496999999999, "isoelectric_point": 9.22402362823486 }, "id": "DFOKDJOJIG_212", "locus": "GCA013367855_00174" }, { "type": "cds", "sequence": "contig_1", "start": 99718, "stop": 99870, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATACTATTTCTATTAGGCTTATTAGTATCATTTGGTTTAAGAAAATTAGTAAGCTTAGTGTTCTTTGCTAACAAGAAGATTGGAGAAATAAATGTCATTAGGAAAATTGAAAATAAAACATTGAAGAGAAAAGTCGTATTTAAGATCTAA", "aa": "MILFLLGLLVSFGLRKLVSLVFFANKKIGEINVIRKIENKTLKRKVVFKI", "aa_hexdigest": "5b9267be914f908d8b08664ff4548470", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5789.191900000001, "isoelectric_point": 11.235179328918456 }, "id": "DFOKDJOJIG_213", "locus": "GCA013367855_00175" }, { "type": "cds", "sequence": "contig_1", "start": 99992, "stop": 100228, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAATGTCATTTGTAGAAAAACAATTAAGTACAGATTCAAGAACCACAATACTATTTCCAAGGGCAATAGTAGAAGGTGGAAAGAGCTTCTTCCCTGAAAACTATAGAGATGCTTTTGAATCAGGATTCCTTGAAGCACTAGTATTTACTGCTGGTATCACAACTTTCTCGGTATTAATTAGATTAGGATTTTCGATTACATTAGGATATGCACTATCATTAAGAAACTGA", "aa": "MIEMSFVEKQLSTDSRTTILFPRAIVEGGKSFFPENYRDAFESGFLEALVFTAGITTFSVLIRLGFSITLGYALSLRN", "aa_hexdigest": "41b59fdb68fc65d361c7dd353b33be8b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8719.945599999992, "isoelectric_point": 5.221082115173339 }, "id": "DFOKDJOJIG_214", "locus": "GCA013367855_00176" }, { "type": "cds", "sequence": "contig_1", "start": 100345, "stop": 100530, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACTGTTCAGGTTGTTGCTGAAACCATTGGCATTGCTATTTTAAAGAAGTATTTGATTCCATTTGCGCCATCTAGCATTGATGATTCTTTAACACTGTTTGGGATTGCTTCAAATGCATTTTTATACATATATCCAAAGAATATTGAAGCAGCAAACGGCATAACTAATGCAAAGAATTGA", "aa": "MQTVQVVAETIGIAILKKYLIPFAPSSIDDSLTLFGIASNAFLYIYPKNIEAANGITNAKN", "aa_hexdigest": "fe5e88f4344ddbf725467d9010363c68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6573.5626, "isoelectric_point": 5.97044734954834 }, "id": "DFOKDJOJIG_215", "locus": "GCA013367855_00177" }, { "type": "cds", "sequence": "contig_1", "start": 100752, "stop": 100976, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_036432692.1", "SO:0001217", "UniParc:UPI00056AF300", "UniRef:UniRef100_A0A6A8Q0V8", "UniRef:UniRef50_A0A454C920", "UniRef:UniRef90_A0A454C920" ], "nt": "ATGCAATTTCAAGACAAGGAAATGCAACAAAAGGATAGAATTATGGAAAAAGACATTAATGTTCAATTAGTAAAATTATCAAAATATCAAAAGAAAAGTAAAATTAAAAAAGTATTTGTTTATATTGGTATAGGATTTGTATTAATTTCTTTGATTGTTATAATGATATTGGGTGTATATGGCATAATTGGTGGTCTAGACGGATTAATAAAAAAAGGTTTATAA", "aa": "MQFQDKEMQQKDRIMEKDINVQLVKLSKYQKKSKIKKVFVYIGIGFVLISLIVIMILGVYGIIGGLDGLIKKGL", "aa_hexdigest": "356c950f18a8b3ca111944fbad47724b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00056AF300", "ncbi_nrp_id": "WP_036432692.1", "uniref100_id": "UniRef100_A0A6A8Q0V8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00056AF300", "RefSeq:WP_036432692.1", "UniRef:UniRef100_A0A6A8Q0V8" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8Q0V8", "uniref90_id": "UniRef90_A0A454C920" }, "psc": { "uniref90_id": "UniRef90_A0A454C920", "uniref50_id": "UniRef50_A0A454C920" }, "pscc": { "uniref50_id": "UniRef50_A0A454C920", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C920" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8444.260299999994, "isoelectric_point": 9.823321723937987 }, "id": "DFOKDJOJIG_216", "locus": "GCA013367855_00178" }, { "type": "cds", "sequence": "contig_1", "start": 101081, "stop": 101236, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCTATGGAACTAAAAATTTATTAATATTTAAGAATAATTTAATTCAAAATAAACAAAATTTAAAATCTTTTGCACTTAAATTAAGCTTTTATGTTTTAAAACAAATGCCACAAATTAAAAGTGTTATATTGGCATATGACAATGAATATTGA", "aa": "MAYGTKNLLIFKNNLIQNKQNLKSFALKLSFYVLKQMPQIKSVILAYDNEY", "aa_hexdigest": "35315d506ca26165cd48c6380d7df148", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5999.050600000001, "isoelectric_point": 9.757241630554198 }, "id": "DFOKDJOJIG_217", "locus": "GCA013367855_00179" }, { "type": "cds", "sequence": "contig_1", "start": 101363, "stop": 102529, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L4", "UniRef:UniRef90_D1J7L4" ], "nt": "TTGGACTTAGCAATATATTTTTCTTTTTTTGACAGTAAATCATGTAAAATTCAAACTATTTTTAAAAATAATCAAGAGATAAATTTTTTTGATTTTAATAATTTCAACCTCAATGAAAATGAATACCAATTTTTAAATGATGAGCCAATATATTTAGATACTAAGATGTTGCTTAATGATTATTTAAACCAAATTACTTCAAATAGAGTAACTAAATATCATAATTCAATCCTTAATTTAATAATTAACAAATATCAATATAATTTTGAATTAATTAAGAAAATTATGGGTTTTGATGATTTGGGATATAAATTTATTAATAAACAAAGATTCACTAATGATACAATTTTTAGAGTTTCTAAAACTTTTTTAACTAGGGGCATGCAGTTTAATTATTTCGCTAGATTTTTTATAAATCAAGAAGTTAAATATTATTTAGCATATAAAAAATTATTTAATTATGATTTTAAACAAATTTTAAATGAACATTTAGTTGCATTATATATTAAATTTCAAAACACATTCTTTAGCTCAATGCTAATAAATGATAATTTTTTTAAAATAAATAAAATTTATATAAACCAACTTTATCCCACTCCTATTTTAATAAAAGTTTGTGAAATGTTTGACATAGAATATAGTTTTGTTTTGCATCAACAAGACATTATTTTAAACGACAATGAAAACGTTTTGTATGTTGATAATAATGATATTTCATTTTTAAGTGCTGAGCATAATTATAGAGATTCATTTAAGACATTTTTTTATATTAATGAAATGTTAAACTATTTTGAAACTCAGCAAATTGATATTTTTGACATTGAAAAAGAGTTGAAATTAAAATTCTTAACTTATAAAGAATTTGAAATTGAATGTAGTATTGCAAATTTAGTTCATTTTAAAGACAAATTAAGTATTCAAAAATCAATATCTCGTATTGAAATTGATCAATTAACAGTTTTTGAAAACAAGCAAGACAAATATATAAAACATATTGCTAAGATAGCATTAAAAACCAATCCTCAATGTTTTATATCTATTAGTTATAATGAAATCTCCTGCAAGCTGAAATTTTCTGTTTTTTATTCGCAAACTACAAAAATTTTCAATAAAATCAAAGAATATTTTAACAAATTTCTTTTATTAAACAAAAAAATTCGCAACTAA", "aa": "MDLAIYFSFFDSKSCKIQTIFKNNQEINFFDFNNFNLNENEYQFLNDEPIYLDTKMLLNDYLNQITSNRVTKYHNSILNLIINKYQYNFELIKKIMGFDDLGYKFINKQRFTNDTIFRVSKTFLTRGMQFNYFARFFINQEVKYYLAYKKLFNYDFKQILNEHLVALYIKFQNTFFSSMLINDNFFKINKIYINQLYPTPILIKVCEMFDIEYSFVLHQQDIILNDNENVLYVDNNDISFLSAEHNYRDSFKTFFYINEMLNYFETQQIDIFDIEKELKLKFLTYKEFEIECSIANLVHFKDKLSIQKSISRIEIDQLTVFENKQDKYIKHIAKIALKTNPQCFISISYNEISCKLKFSVFYSQTTKIFNKIKEYFNKFLLLNKKIRN", "aa_hexdigest": "f02c3df6cbe66acf65e4d78e18b5bc8d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7L4", "query_cov": 1.0, "subject_cov": 0.8049792531120332, "identity": 0.9790000000000001, "score": 746.0, "evalue": 2.24e-270, "valid": true, "uniref50_id": "UniRef50_D1J7L4" }, "pscc": { "uniref50_id": "UniRef50_D1J7L4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 47142.7263, "isoelectric_point": 8.473353767395022 }, "id": "DFOKDJOJIG_218", "locus": "GCA013367855_00180" }, { "type": "cds", "sequence": "contig_1", "start": 102546, "stop": 102938, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACACAACCATTGAATAATTTAGAGCTTTATAAAGTTGATAGTAAGGGTAAAAAGATAAATGTTCAAAGATTCCCTGATGGTACAAAAGCAATAGATGCTTTTAATATCTTTAGTGGTTATATTTCTCCTACAACTAAATTTCTAAGCCAAGATGCAAAGAACGTAACAAAAAATATTGCCGAAGAAATAGCATTCACAAACTTTAAACACTTAATCAAAGAAGGATCAATATACAAACCTGCCGATGATGTTGCAACTAGAAGAAGTGGTCAATTAAGAGATGCAATAAAGCAAGCAGCTAGATCAATATTGAGTGATGCCAATGTTTTAAAAATTTTTGATTACAATGAATTTATTAATAAAATTAAATTATTATTTCAAGAATAA", "aa": "MYTQPLNNLELYKVDSKGKKINVQRFPDGTKAIDAFNIFSGYISPTTKFLSQDAKNVTKNIAEEIAFTNFKHLIKEGSIYKPADDVATRRSGQLRDAIKQAARSILSDANVLKIFDYNEFINKIKLLFQE", "aa_hexdigest": "0ea137e972fd1eaea1e60d5716f41773", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14808.76529999998, "isoelectric_point": 9.396863365173338 }, "id": "DFOKDJOJIG_219", "locus": "GCA013367855_00181" }, { "type": "cds", "sequence": "contig_1", "start": 103437, "stop": 103589, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTACTTCAACCGCTGCTTATCATTACACTTTTGTTAGTGAAAATGGAAAAAATAACAATTATACAATCAAGCAGGCAAACAGAGTGTTAAATCCAAACACAACAATTGAAATATTAAACGATACTCCCAAAAATAATGAAAAATGA", "aa": "MVITSTAAYHYTFVSENGKNNNYTIKQANRVLNPNTTIEILNDTPKNNEK", "aa_hexdigest": "e555693429336f0243fbba90845c7f61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5686.239400000001, "isoelectric_point": 8.085963249206543 }, "id": "DFOKDJOJIG_220", "locus": "GCA013367855_00182" }, { "type": "cds", "sequence": "contig_1", "start": 103644, "stop": 103955, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAATTATTTGACAACTATAGCGATTTAATTAATTTTGCAATTCAAGCAAAAAGATTATACAACGCGGACCCTACATTAAATGTTTTAGGAATAGATGAAATACCTTCGGCTATCAATGTTATGGCAACATCAATCATTGGTAGTGATATCAATAAAAACTATATAAACCCAGATGATAATTGCCCAACAACTGGTGGCTACAATTATGACAATTTTATTGAAAAACCTGATAGCCCGCAAGCGAAATTATTTAAAAAAGTTTCTTCTATTATTTTAAAGGGTATTCAAGAAGGTGCCGTTTGA", "aa": "MSDKLFDNYSDLINFAIQAKRLYNADPTLNVLGIDEIPSAINVMATSIIGSDINKNYINPDDNCPTTGGYNYDNFIEKPDSPQAKLFKKVSSIILKGIQEGAV", "aa_hexdigest": "9234b174f3d6518ffd346b45e2cb6725", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11330.66199999999, "isoelectric_point": 4.520598411560059 }, "id": "DFOKDJOJIG_221", "locus": "GCA013367855_00183" }, { "type": "cds", "sequence": "contig_1", "start": 104025, "stop": 104312, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTAGCAATTGATGACAAAGATTTTAAAAAATATAACATTGAACCTGTATTTAATGTTGCAAACACTTTAATTGCAAATAACAAATATAATAAAAAGTGGGCAATACCTCTATCTCGTTCAGGAGAAATGACTACAGTAGCTAAAGCATTAGTTGGAAAATTCGTTAAAGAATTAATGGCTCTAGGAGTTAAATTAGATAGTCAAAATAGTAGCAAATTGCAATCTTATTTAGACTATTACAATAATAACGATCAAGAAGCAAAAGGCGTTGATGGTATTTGA", "aa": "MNLAIDDKDFKKYNIEPVFNVANTLIANNKYNKKWAIPLSRSGEMTTVAKALVGKFVKELMALGVKLDSQNSSKLQSYLDYYNNNDQEAKGVDGI", "aa_hexdigest": "6f7ef2b518ad754bb9c7f2dd8b309ec5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10663.046899999994, "isoelectric_point": 8.802851676940918 }, "id": "DFOKDJOJIG_222", "locus": "GCA013367855_00184" }, { "type": "cds", "sequence": "contig_1", "start": 104487, "stop": 104696, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGACTTGGCAAATTATTCACTTTAATGACTATAGGTTTATTTCCTACGACTACATTGATTTTATCCTCATGTTCAACAACAGAAAATTCGAAATACGGATTTGACCAAGAAGATGATGGACAATTAGTATTAGCAATGGGATTTTTAAAAAATGATTGGCAAGGGCTTGCTATTCAAGGGATTGTTGATACATACAACAAATGA", "aa": "MKRLGKLFTLMTIGLFPTTTLILSSCSTTENSKYGFDQEDDGQLVLAMGFLKNDWQGLAIQGIVDTYNK", "aa_hexdigest": "9d9e937aeae4b0fa113f522ac9114c10", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7679.7734, "isoelectric_point": 4.9838361740112305 }, "id": "DFOKDJOJIG_223", "locus": "GCA013367855_00185" }, { "type": "cds", "sequence": "contig_1", "start": 104955, "stop": 105347, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACACAACCATTTAATAATTTAGAACTTTATAAAGTTGATAGCAATGGCAAAAAAACAAACATTCAAAAATTCCCTGATGGTACAAAAGCAATAGATGCTTTCAACGCCTTTGGTGGATATATTTCTCCTACAAATAAATATCTAAGTCAAGATGCAAAGAGTGCAACAAAAAATATTGCTGATGAAATAGCATTCACAAACTTTAAACATTTAATCAATGAAGGATCAATATACAAGCCTGCCGATGATATTGCAACTAGAAAAGGCGACCAATTAAGGGATGCAATAAAGCAAGCAGCCAGATCAATATTGAGTGATGCCAATGTTTTAAAAGTTTTTGACTACAAAGATTTTATTAATAAAATTAAGCTATTGTTCCAAGAATAA", "aa": "MYTQPFNNLELYKVDSNGKKTNIQKFPDGTKAIDAFNAFGGYISPTNKYLSQDAKSATKNIADEIAFTNFKHLINEGSIYKPADDIATRKGDQLRDAIKQAARSILSDANVLKVFDYKDFINKIKLLFQE", "aa_hexdigest": "5ab6a5f8d8d4a9c4beef82b4c1727227", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14676.428799999978, "isoelectric_point": 9.001930046081544 }, "id": "DFOKDJOJIG_224", "locus": "GCA013367855_00186" }, { "type": "cds", "sequence": "contig_1", "start": 105474, "stop": 105701, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGGCAAGGCAGGCTATATAGTTTCCGGTTCTGAATATTCATACAATAAAGCCGAAAATAAAATTGAATTAAAAATAGGAAATAGAATAACTAAATTACAAGGAATTTATTTAGGTAAATTCTTTAATAATAATGAAAATGAATATTTCTTTATTAATCAAGAAGACGTGGATGTTAAAGTATTAAAGGCCAATAATTTTATTAACCAAGAAGATGCAGATTGA", "aa": "MQGKAGYIVSGSEYSYNKAENKIELKIGNRITKLQGIYLGKFFNNNENEYFFINQEDVDVKVLKANNFINQEDAD", "aa_hexdigest": "ed1377f3634eab878203d13701e5c1e6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8644.516199999995, "isoelectric_point": 5.049485206604003 }, "id": "DFOKDJOJIG_225", "locus": "GCA013367855_00187" }, { "type": "cds", "sequence": "contig_1", "start": 105846, "stop": 106004, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATAAATATTGGTTCTACTGCTGGATATCGTCACACATTCGTTGGCAATAATGAAAAAAATAGTACTTATATAATTAAACAAGCCAACAGGGTATTAGATCCAACAACAACAATTGAAATATTAAATAATACTCCTAAAAATAATGAAAAATGA", "aa": "MAINIGSTAGYRHTFVGNNEKNSTYIIKQANRVLDPTTTIEILNNTPKNNEK", "aa_hexdigest": "9f125fe2d5c33ffda7d748534f0afbda", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5806.436100000001, "isoelectric_point": 9.301836967468262 }, "id": "DFOKDJOJIG_226", "locus": "GCA013367855_00188" }, { "type": "cds", "sequence": "contig_1", "start": 106053, "stop": 106364, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAATTATTTGACAACTATAGCGATTTAATTAACTTTGCAATTCAAGCAAAAAGATTATATAATGTTGACCCTACATTAAATGTTTTGGGAATAGATGAAATACCTTCGGCTATCAATGTTATGGCAACATCAATCATTGGTAGTGATATCAATAAAAACTATATAAACCCAGATGATAATTGCCCTGCAACTGGCGGCTACAATTATGACAATTTTATTGAAAATCCTAATAGTCCACAAGCAAAATTATTTAAAAAAGTTTCTTCTATTATTTTAAAAGGTATCTCAGAAAATGCTGTTTGA", "aa": "MSDKLFDNYSDLINFAIQAKRLYNVDPTLNVLGIDEIPSAINVMATSIIGSDINKNYINPDDNCPATGGYNYDNFIENPNSPQAKLFKKVSSIILKGISENAV", "aa_hexdigest": "c95348207eb1ab4dee7b8d8295cdda18", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11329.633999999987, "isoelectric_point": 4.478082847595215 }, "id": "DFOKDJOJIG_227", "locus": "GCA013367855_00189" }, { "type": "cds", "sequence": "contig_1", "start": 106434, "stop": 106541, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTAGGAGTTAAATTAGATAGTAAAAATAGCAGCAAATTGCAATCTTATTTAGACTATTACAATAATAATAAGCAAGAAGCAGAAGGCGTTGATAGTATTTGA", "aa": "MALGVKLDSKNSSKLQSYLDYYNNNKQEAEGVDSI", "aa_hexdigest": "79ceb1d1e6b11b63495da1f55eaf8c03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3923.2740000000013, "isoelectric_point": 4.8610639572143555 }, "id": "DFOKDJOJIG_228", "locus": "GCA013367855_00190" }, { "type": "cds", "sequence": "contig_1", "start": 106617, "stop": 106808, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACCTAGAAACAAAAAACAGAAAAGATTTTCACAATATTTTATTAAATTACGCATCAGCAGCTGCTTTATATGCAAACCATAAAATGAATTTAGCGGTTGATGACAAAGATTTTAAGAAATATAACATCGAACCTGTATTTAATAATGCAAACTCTTTAATTGCAAATAATAAAGATAACAAAAAATGA", "aa": "MDLETKNRKDFHNILLNYASAAALYANHKMNLAVDDKDFKKYNIEPVFNNANSLIANNKDNKK", "aa_hexdigest": "54875efe247097299132ad564505a3ee", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7271.124200000002, "isoelectric_point": 8.964280509948733 }, "id": "DFOKDJOJIG_229", "locus": "GCA013367855_00191" }, { "type": "cds", "sequence": "contig_1", "start": 106908, "stop": 107117, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTACCAAATTATTTACAGTAGTAGCAGCAGGATTATTTCCCGCAACTGCATTAATTTCAGCCTCGTGCTCAACAACAGAAAATTCAAAATATGGATTTGACCAAGATGATGATGGACAATTAGTATTAGCAACAGGATTTTCAAAAAGTAACTGGCAAGGATTTGCCATTCAAGGAATTGTTAATACATATAATAATTGA", "aa": "MKKITKLFTVVAAGLFPATALISASCSTTENSKYGFDQDDDGQLVLATGFSKSNWQGFAIQGIVNTYNN", "aa_hexdigest": "b8fbe58d705f7321fc7a16456b18d2c4", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7396.236200000001, "isoelectric_point": 5.952713584899903 }, "id": "DFOKDJOJIG_230", "locus": "GCA013367855_00192" }, { "type": "cds", "sequence": "contig_1", "start": 107528, "stop": 108022, "strand": "+", "frame": 2, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "SO:0001217", "UniRef:UniRef50_Q9PPZ2", "UniRef:UniRef90_D1J7L7" ], "nt": "ATGTCAAAATTTGTTGGCTCAATGTTTAAAAAGAGCCGTAGATATGGAATTTCTTTATTAGAAAATAATAAAGAATTCACAAAAGGCAAAAAACGTACAACTGCCCCTGGACAACACGGAGCAAAAAGAAGCAAACCGTCAGACTATCAACTACACATGTATGAAAAACAAAAAGTTAGATATATGTATGGTTTAAATGAACGTCAATTTAAAAATTTATTTATTAAAGTTTCTAAAAAAGCCGGAGTTACTGGTATTAACTTGCTACAAACTGTTGAATCAAGATTAGATAATCTAGTATTTAGAGCAGGATTTGCAAGAACAAGAGCTCAAGCAAGACAATTAGTAAATCATGGACACTTCACAGTTGATGGATGCAAAGCAAACATTCCTTCAATGATTGTTAAAGTAGGTTCAGTTATTGAATTAAAAGAAGCATTACAAAATAATGTTGCTATTAAAGATGCTATTGAAGTAATGACAGTTGCTCCATGA", "aa": "MSKFVGSMFKKSRRYGISLLENNKEFTKGKKRTTAPGQHGAKRSKPSDYQLHMYEKQKVRYMYGLNERQFKNLFIKVSKKAGVTGINLLQTVESRLDNLVFRAGFARTRAQARQLVNHGHFTVDGCKANIPSMIVKVGSVIELKEALQNNVAIKDAIEVMTVAP", "aa_hexdigest": "da01c9dae17b2a1d09586afb5cf50a64", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7L7", "query_cov": 1.0, "subject_cov": 0.8241206030150754, "identity": 1.0, "score": 317.0, "evalue": 4.69e-109, "valid": true, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_Q9PPZ2", "cog_id": "COG0522", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "DFOKDJOJIG_231", "locus": "GCA013367855_00193" }, { "type": "cds", "sequence": "contig_1", "start": 108167, "stop": 108478, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63", "UniRef:UniRef90_UPI000AFE6B63" ], "nt": "TTGTTTGCTTTTGTAATTATATTAATATATCTAATATCACTTATTTTGGGTATTATTTTTTATCAAATTAGTCATGATAGACTCACAAACTATTTGATTATTGTTTTAAGTTCTATAGTTTTCCCTGAAATTTTAAATACGCTGTTTTCACTTTCAAATATAACAATGACGCTTAATCCTTTATGCAATAATATTTATCTAATTAATTTAGTTTATATACCCGCATTTATCTTATTTATTGTTTTAATTACAAAAACAAAAGAAAAACAATTTATATTTTTTAAAAAAGTTAATTATGTTAAAAATAAATAA", "aa": "MFAFVIILIYLISLILGIIFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNPLCNNIYLINLVYIPAFILFIVLITKTKEKQFIFFKKVNYVKNK", "aa_hexdigest": "0d1dedecda8e873ddc94679aa4aee80e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 108167, "stop": 108664, "observations": { "mutation": [ 108535 ], "stop": [ 108535 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AFE6B63", "query_cov": 1.0, "subject_cov": 0.6242424242424243, "identity": 0.961, "score": 174.0, "evalue": 4.06e-54, "gene_start": 1, "gene_end": 103, "reference_start": 63, "reference_end": 165, "reference_sequence": "MILFINQSYYRIKDGRDHSKTPVYLENTKIIEYFYVYIFRLTYWFLLSFLLIKITNNFKKAILFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNSLCNNIYLINLVYIPAFILFIVLITKTKEKQFIFFKKVNYVKNK" }, "paralog": false, "identity": 0.9757575757575757, "score": 315.0, "evalue": 7.48e-112, "description": "Internal stop codon at 108535. Nonsense mutation around 108535.", "psc": { "uniref90_id": "UniRef90_UPI000AFE6B63", "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AFE6B63", "UniRef:UniRef50_UPI000AFE6B63" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12068.58619999998, "isoelectric_point": 9.519675636291506 }, "id": "DFOKDJOJIG_232", "locus": "GCA013367855_00194" }, { "type": "cds", "sequence": "contig_1", "start": 108533, "stop": 108664, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTATTTATTAATCAAAGTTATTATAGAATAAAGGATGGAAGGGATCATTCAAAAACTCCTGTATATTTAGAAAATACAAAAATCATTGAATATTTTTATGTTTATATTTTTAGATTAACCTATTGA", "aa": "MILFINQSYYRIKDGRDHSKTPVYLENTKIIEYFYVYIFRLTY", "aa_hexdigest": "08c0e391f8f9e0388870c03b597d3522", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5416.208799999999, "isoelectric_point": 9.048863029479982 }, "id": "DFOKDJOJIG_233", "locus": "GCA013367855_00195" }, { "type": "cds", "sequence": "contig_1", "start": 109196, "stop": 109429, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241147D1", "UniRef:UniRef90_UPI00241147D1" ], "nt": "GTGATAGAAAAATCTCTTTATGATTTTGCGACCCCTGATTTTCAAACACTTCCAAATAATATTATTAAACCCTTTTATATATATTGCAACCCAGAAGATGCAAAAAAGGAACTTGAACAACTATTTAAAACATTAGATAATACAAAATACGAAGCATTACTATTAGAAGCAGCAAATACTTTTAGTTTCGAATTAAAAAAATTTAATGAAGAATATTTAAACAAATCTAGTTGA", "aa": "MIEKSLYDFATPDFQTLPNNIIKPFYIYCNPEDAKKELEQLFKTLDNTKYEALLLEAANTFSFELKKFNEEYLNKSS", "aa_hexdigest": "1f826f4c54ae47a74e7e0902cc4d2199", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 109112, "stop": 109429, "observations": { "mutation": [ 109162, 109198 ], "stop": [ 109162, 109198 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00241147D1", "query_cov": 1.0, "subject_cov": 0.7333333333333333, "identity": 1.0, "score": 156.0, "evalue": 4.31e-48, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MIEKSLYDFATPDFQTLPNNIIKPFYIYCNPEDAKKELEQLFKTLDNTKYEALLLEAANTFSFELKKFNEEYLNKSSWFYNYIHNQMSLWIEKANDIIFKKLKNN" }, "paralog": false, "identity": 0.9714285714285714, "score": 207.0, "evalue": 9.28e-71, "description": "Internal stop codon at 109162, 109198. Nonsense mutation around 109162, 109198.", "psc": { "uniref90_id": "UniRef90_UPI00241147D1", "uniref50_id": "UniRef50_UPI00241147D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00241147D1", "UniRef:UniRef50_UPI00241147D1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00241147D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241147D1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9111.225099999996, "isoelectric_point": 4.705097770690917 }, "id": "DFOKDJOJIG_234", "locus": "GCA013367855_00196" }, { "type": "cds", "sequence": "contig_1", "start": 110101, "stop": 110463, "strand": "-", "frame": 1, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005A7E644", "UniRef:UniRef50_UPI0013142BC0", "UniRef:UniRef90_UPI0005A7E644", "UniRef:UniRef90_UPI0013142BC0" ], "nt": "ATGGTTAGAATGGTTGGATTTCACTCTGGATATTTAAACCAAAATAACCATATGTTATTAAATAAAACCATTATTTTTCAGTTTAACAAAAGTCAAAAAATTTATCAAAATATAGATATCTTAGCCACTAAAACCAGCGAAATTTTAAATAACTTTTACCATGCAAAAACTATTAATTTATCAGGAGATGGAGCAAGAATAATAACTAAATTTAGCGAACAAATTAATGCTATTAGGTATTATGATAAGTTCCATTTTCACAAAATATTATTTGATGTTTTTGGGTATTCAAGAAAGAAAAATAAGGATAATAAGAGAATATTTGATAATACATTTGGGAATCAATTTTCTCAATTTTTGTGA", "aa": "MVRMVGFHSGYLNQNNHMLLNKTIIFQFNKSQKIYQNIDILATKTSEILNNFYHAKTINLSGDGARIITKFSEQINAIRYYDKFHFHKILFDVFGYSRKKNKDNKRIFDNTFGNQFSQFL", "aa_hexdigest": "cf62241933c3fbb644ce3d03a7aa80fc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0005A7E644", "query_cov": 0.975, "subject_cov": 1.0, "identity": 0.94, "score": 226.0, "evalue": 6.52e-75, "valid": true, "uniref50_id": "UniRef50_UPI0005A7E644" }, "pscc": { "uniref50_id": "UniRef50_UPI0005A7E644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005A7E644" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 109798, "stop": 110553, "observations": { "mutation": [ 109980, 110103 ], "stop": [ 109980, 110103 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013142BC0", "query_cov": 1.0, "subject_cov": 0.47244094488188976, "identity": 0.992, "score": 241.0, "evalue": 4.85e-79, "gene_start": 1, "gene_end": 120, "reference_start": 31, "reference_end": 150, "reference_sequence": "MKNTKFLPSKNDVIQLENDDAYINLNKGKFMVRMVGFHSGYLNQNNHMLLNKTIILQFNKSQKIYQNIDILATKTSEILNNFYHAKTINLSGDGARIITKFSEQINAIRYYDKFHFHKILFDVFGYSRKKNKDNKRIFDNTFGNQFSQFLWFLNNKKYEDFENKLHENLIYLRENKLSAIKIVEIKSLIKLWKNNKNYLINSYENANYYGGNAETFIGHYLKKYTKTAFSRHNIDYLKLKILLNIPQNLNVVFL" }, "paralog": false, "identity": 0.9840637450199203, "score": 499.0, "evalue": 4.17e-183, "description": "Internal stop codon at 109980, 110103. Nonsense mutation around 109980, 110103.", "psc": { "uniref90_id": "UniRef90_UPI0013142BC0", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_UPI0013142BC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013142BC0", "UniRef:UniRef50_UPI0013142BC0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013142BC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013142BC0" ], "product": "Mbov_0401 family ICE element transposase-like protein" } }, "id": "DFOKDJOJIG_235", "locus": "GCA013367855_00197" }, { "type": "cds", "sequence": "contig_1", "start": 110839, "stop": 110979, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATAGATTTTTATATTAATGGTGTAAGCGAAAAAAGTATCTTATCCTCAGGCCAATTAATAGTAGAAAAATTGAACATCGAAGAAGAAATATTTCGTACTAAAACAAGGTTTGAAAATAATCAATATAGTAATTGA", "aa": "MEIDFYINGVSEKSILSSGQLIVEKLNIEEEIFRTKTRFENNQYSN", "aa_hexdigest": "da1f63fc5604fbd82d1459e9fb83b08a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5428.0015, "isoelectric_point": 4.644962120056152 }, "id": "DFOKDJOJIG_236", "locus": "GCA013367855_00198" }, { "type": "cds", "sequence": "contig_1", "start": 111016, "stop": 111636, "strand": "-", "frame": 1, "gene": null, "product": "Mbov_0400 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0", "UniRef:UniRef90_UPI00069702F0" ], "nt": "ATGAATCATCAAAGTGTAGTAGTCACCAAAGATATTTTTATAATTGATAAAGATGTTTTAGAAGCCAATGTAGATCAAAACATCTATCAAGAAACCTTGACATTAAATAAAAACGATAAATCTTTAATAATTAGCGATTTAGAACAAAGGATTAATTCAATTCCACCTGATTTAAATATTTTAAAAATATCTAATAACTTAAAAAGCAATTTAGTTTTATACACTAATGATAGCCTAGTTAATTCTCAGTCTAATTCGATTTTGAAAGGCAAAAATTACAAAATTAAAAAGGCTACTAAAAATTATTACAAACATAATTTTAACAAATCTCAATTTCTTAAAGATACAAACACGTCAAATATAGAACATACTAAGCAAGCCCTAAAAAACATAAAGTTATGCATAAATAAAGCTAAAAAAATTGATTATACTAATTTTGAATATATTTTAGATGTTCAAACAAACACATATCTATTTCATCTAATTCCCTCTTCTCTATCCGATTTTGATAAAACACCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACCAATCTCAGAAAGAGTCCAAAGCTAGCCAATTCCGCTCAAAACCTCATAATCTAAAACATTAG", "aa": "MNHQSVVVTKDIFIIDKDVLEANVDQNIYQETLTLNKNDKSLIISDLEQRINSIPPDLNILKISNNLKSNLVLYTNDSLVNSQSNSILKGKNYKIKKATKNYYKHNFNKSQFLKDTNTSNIEHTKQALKNIKLCINKAKKIDYTNFEYILDVQTNTYLFHLIPSSLSDFDKTPTDLQDSKVINEYNQSQKESKASQFRSKPHNLKH", "aa_hexdigest": "3769969c99795e10e2a3819124aca02d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 111016, "stop": 111858, "observations": { "mutation": [ 111639, 111843 ], "stop": [ 111639, 111843 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00069702F0", "query_cov": 1.0, "subject_cov": 0.7357142857142858, "identity": 1.0, "score": 389.0, "evalue": 1.44e-135, "gene_start": 1, "gene_end": 206, "reference_start": 75, "reference_end": 280, "reference_sequence": "MSLELWKPLKITNPLLEDIFHLNIDTKFKIHPLIIFENKENKTYYCIRLQTSTKTTKKHNLQIDNSSYQTNEYWMNHQSVVVTKDIFIIDKDVLEANVDQNIYQETLTLNKNDKSLIISDLEQRINSIPPDLNILKISNNLKSNLVLYTNDSLVNSQSNSILKGKNYKIKKATKNYYKHNFNKSQFLKDTNTSNIEHTKQALKNIKLCINKAKKIDYTNFEYILDVQTNTYLFHLIPSSLSDFDKTPTDLQDSKVINEYNQSQKESKASQFRSKPHNLKH" }, "paralog": false, "identity": 0.9928571428571429, "score": 553.0, "evalue": 1.03e-202, "description": "Internal stop codon at 111639, 111843. Nonsense mutation around 111639, 111843.", "psc": { "uniref90_id": "UniRef90_UPI00069702F0", "product": "Mbov_0400 family ICE element protein", "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00069702F0", "UniRef:UniRef50_A0A454C8W0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0" ] } }, "id": "DFOKDJOJIG_237", "locus": "GCA013367855_00199" }, { "type": "cds", "sequence": "contig_1", "start": 112909, "stop": 113262, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358", "UniRef:UniRef50_UPI0027330C60", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef90_UPI001314B421" ], "nt": "TTGGAATTAGTTTCAAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAAGCCCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTTGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATACAAAAAAATTCTTTTATTACCAACAAACAAAACAATCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATTGACCCTGATTGA", "aa": "MELVSKYDDPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPD", "aa_hexdigest": "c9f7e66eb051396304f5653fddd5b1cc", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001314B421", "query_cov": 0.8632478632478633, "subject_cov": 0.9805825242718447, "identity": 0.95, "score": 178.0, "evalue": 3e-56, "valid": true, "uniref50_id": "UniRef50_UPI0027330C60" }, "pscc": { "uniref50_id": "UniRef50_UPI0027330C60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027330C60" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 112627, "stop": 113238, "observations": { "mutation": [ 112728, 112815, 112818, 112911 ], "stop": [ 112728, 112815, 112818, 112911 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005C90358", "query_cov": 0.9316239316239316, "subject_cov": 0.4448979591836735, "identity": 0.991, "score": 208.0, "evalue": 3.74e-66, "gene_start": 9, "gene_end": 117, "reference_start": 1, "reference_end": 109, "reference_sequence": "NPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPDWKPNPNPDDEDIVSPEDIPKDPNRIKKVVKTWWFPLVVIVGILTFLVASIFIIKSLLRKYGWSFGKRAKRIRRQNPNKQSYQEKRSNKRQEKLAKKEAKNLKRQTKKQEKANFIQAKKDKKTKNKSKTNNEQNSIN" }, "paralog": false, "identity": 0.9605911330049262, "score": 382.0, "evalue": 3.61e-137, "description": "Internal stop codon at 112728, 112815, 112818, 112911. Nonsense mutation around 112728, 112815, 112818, 112911.", "psc": { "uniref90_id": "UniRef90_UPI0005C90358", "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef50_UPI0005C90358" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13938.78549999998, "isoelectric_point": 5.6632894515991214 }, "id": "DFOKDJOJIG_238", "locus": "GCA013367855_00200" }, { "type": "cds", "sequence": "contig_1", "start": 113263, "stop": 113790, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCAGAAGATGTTATTGATGAAAGATATAGAAAAGCAATAGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCCAATTTAAATGAAGTAATTGCCCATTTAATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTTTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGA", "aa": "MDIQSEFILNISKKTFEEKNIPEDVIDERYRKAIEKEVINFITNQLLQYQLKTKSSGKEIEMLLNKDVEIANLNEVIAHLMRGKALSSFVGLKLVSKNANLHNYKIINFKNLGEGGKFNLRNLSYILMDNKNVIQETKPSQIRKKVIEIVVNAASKLGLEINNDYQIFAINNDAY", "aa_hexdigest": "16cb6b67b60f78684bdb9d0db65ba5e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20119.024600000004, "isoelectric_point": 8.923278617858887 }, "id": "DFOKDJOJIG_239", "locus": "GCA013367855_00201" }, { "type": "cds", "sequence": "contig_1", "start": 113824, "stop": 114048, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823", "UniRef:UniRef90_UPI001314F823" ], "nt": "ATGTCAAGCAACACATTAACCAAAAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACTGAGGAAAATAAATTCCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGA", "aa": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEA", "aa_hexdigest": "4cf89e55fbd43bc2c9828114fb6c5739", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 113598, "stop": 114048, "observations": { "insertion": [ 113637 ], "mutation": [ 113826 ], "stop": [ 113826 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314F823", "query_cov": 1.0, "subject_cov": 0.4966442953020134, "identity": 1.0, "score": 145.0, "evalue": 1.93e-43, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEAWHVYNPPIDLSTLDIQNEFILNISKKTFEEKNIPEDVVDERYRKAIEKEVINFITNQLLQYQLKQNLLAKKLKCY" }, "paralog": false, "identity": 0.9798657718120806, "score": 287.0, "evalue": 1.32e-101, "description": "Internal stop codon at 113826. Frameshift due to insertion around 113637. Nonsense mutation around 113826.", "psc": { "uniref90_id": "UniRef90_UPI001314F823", "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314F823", "UniRef:UniRef50_UPI001314F823" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8600.459199999998, "isoelectric_point": 4.854925346374511 }, "id": "DFOKDJOJIG_240", "locus": "GCA013367855_00202" }, { "type": "cds", "sequence": "contig_1", "start": 114190, "stop": 114543, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358", "UniRef:UniRef50_UPI0027330C60", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef90_UPI001314B421" ], "nt": "TTGGAATTAGTTTCGAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAGGCTCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTCGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATATAAGAAAATTCTTTTATTACCTACAAACAAAACCACCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATAGACCCTGATTGA", "aa": "MELVSKYDDPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTTGFGYGYIINKANKEYNPAIDPD", "aa_hexdigest": "7338646e778a5dfe217af27c3d57dad9", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001314B421", "query_cov": 0.8632478632478633, "subject_cov": 0.9805825242718447, "identity": 0.96, "score": 179.0, "evalue": 1.05e-56, "valid": true, "uniref50_id": "UniRef50_UPI0027330C60" }, "pscc": { "uniref50_id": "UniRef50_UPI0027330C60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027330C60" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 114157, "stop": 114519, "observations": { "mutation": [ 114192 ], "stop": [ 114192 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005C90358", "query_cov": 0.9316239316239316, "subject_cov": 0.4448979591836735, "identity": 0.982, "score": 205.0, "evalue": 6.14e-65, "gene_start": 9, "gene_end": 117, "reference_start": 1, "reference_end": 109, "reference_sequence": "NPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPDWKPNPNPDDEDIVSPEDIPKDPNRIKKVVKTWWFPLVVIVGILTFLVASIFIIKSLLRKYGWSFGKRAKRIRRQNPNKQSYQEKRSNKRQEKLAKKEAKNLKRQTKKQEKANFIQAKKDKKTKNKSKTNNEQNSIN" }, "paralog": false, "identity": 0.975, "score": 239.0, "evalue": 6.85e-81, "description": "Internal stop codon at 114192. Nonsense mutation around 114192.", "psc": { "uniref90_id": "UniRef90_UPI0005C90358", "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef50_UPI0005C90358" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13926.73179999998, "isoelectric_point": 5.6632894515991214 }, "id": "DFOKDJOJIG_241", "locus": "GCA013367855_00203" }, { "type": "cds", "sequence": "contig_1", "start": 114544, "stop": 115071, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCAGAAGATGTTATTGATGAAAGATATAGAAAAGCAATAGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCCAATTTAAATGAAGTAATTGCCCATTTAATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTCTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGA", "aa": "MDIQSEFILNISKKTFEEKNIPEDVIDERYRKAIEKEVINFITNQLLQYQLKTKSSGKEIEMLLNKDVEIANLNEVIAHLMRGKALSSFVGLKLVSKNANLHNYKIINFKNLGEGGKFNLRNLSYILMDNKNVIQETKPSQIRKKVIEIVVNAASKLGLEINNDYQIFAINNDAY", "aa_hexdigest": "16cb6b67b60f78684bdb9d0db65ba5e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20119.024600000004, "isoelectric_point": 8.923278617858887 }, "id": "DFOKDJOJIG_242", "locus": "GCA013367855_00204" }, { "type": "cds", "sequence": "contig_1", "start": 115105, "stop": 115329, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823", "UniRef:UniRef90_UPI001314F823" ], "nt": "ATGTCAAGCAACACATTAACCAAAAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACCGAGGAAAATAAATTTCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGA", "aa": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEA", "aa_hexdigest": "4cf89e55fbd43bc2c9828114fb6c5739", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 114879, "stop": 115329, "observations": { "insertion": [ 114918 ], "mutation": [ 115107 ], "stop": [ 115107 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314F823", "query_cov": 1.0, "subject_cov": 0.4966442953020134, "identity": 1.0, "score": 145.0, "evalue": 1.93e-43, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEAWHVYNPPIDLSTLDIQNEFILNISKKTFEEKNIPEDVVDERYRKAIEKEVINFITNQLLQYQLKQNLLAKKLKCY" }, "paralog": false, "identity": 0.9798657718120806, "score": 287.0, "evalue": 1.32e-101, "description": "Internal stop codon at 115107. Frameshift due to insertion around 114918. Nonsense mutation around 115107.", "psc": { "uniref90_id": "UniRef90_UPI001314F823", "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314F823", "UniRef:UniRef50_UPI001314F823" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8600.459199999998, "isoelectric_point": 4.854925346374511 }, "id": "DFOKDJOJIG_243", "locus": "GCA013367855_00205" }, { "type": "cds", "sequence": "contig_1", "start": 115756, "stop": 115989, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAATGATTTTGGTATTTTTGCAGAGGGCGCTGTCCTTGGTAAAGGAGCCTTAAGAAATCTAATCTTAGATAAAAATATTGCCAAAAAATCTAATGTTACATACTATAAATGCAAAATTTATGATAAAGGAAGCAACAAATGGCTTAGTCCTAGTGAAATTAAACCTGAACCATTATCTTCATCAAACGGTAATAGTGAAAATGCTTATATGTCTGAAGAAGGCATCTGA", "aa": "MLNDFGIFAEGAVLGKGALRNLILDKNIAKKSNVTYYKCKIYDKGSNKWLSPSEIKPEPLSSSNGNSENAYMSEEGI", "aa_hexdigest": "b45baf31aa37607b88b8d8fbcdf77cef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8459.5085, "isoelectric_point": 7.798563194274903 }, "id": "DFOKDJOJIG_244", "locus": "GCA013367855_00206" }, { "type": "cds", "sequence": "contig_1", "start": 116212, "stop": 116559, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATACTCCACTACAAGTAAATTTTGACACCACATTAGAAGAAAATGAAGTTTTATTTATAAATGGTCAAAAAGTTGATGTTTTAAATAGACATTTTGTTTATCATTTAAAAGATTTAAGACGTTCGGCATCCGATGAAGAAAAAATAGCATTAGAGAAAAATGATTCAAATAAGCCTCAAGAAGAGTTAAATGACGATAATTCAAGAAAGAAAAACGAGTATATAGTTGAAATAAAGAAATTCAAACCTGGCACAAATAATAGCGGAGAGCCAATAGCAACTTATAAAATTAAGTTCGTTATAGTTGAACAACCAATCAACCAATCATTTAAATGGTTTGCTTGA", "aa": "MHTPLQVNFDTTLEENEVLFINGQKVDVLNRHFVYHLKDLRRSASDEEKIALEKNDSNKPQEELNDDNSRKKNEYIVEIKKFKPGTNNSGEPIATYKIKFVIVEQPINQSFKWFA", "aa_hexdigest": "8533c2db2702be0af9ebac919fac8f16", "start_type": "GTG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13495.009399999984, "isoelectric_point": 5.925374031066894 }, "id": "DFOKDJOJIG_245", "locus": "GCA013367855_00207" }, { "type": "cds", "sequence": "contig_1", "start": 116701, "stop": 117198, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGCATTTCATCTACAAAATTCAAGTCAAGAAATTATTATAGATTCTGATACTATGGGAAAATTAACGACAGGTATTGATTTAAATATTTTTAAAGGTGGTAAAAGTAATCTTGAAAAACTACAAGAAATTGTTGAACAAATGGATAATGAAACAGCAATTGGCAATTTTATTATTAAATATGGTTATGAAGTCAAACCTATTTCAGATGGAAATCATTTTAATCTAATTTTTAAAATCAAACAATTAAAATATAAGGGAAAAGATATCACTAATTCTTTTGCTTCTAAAATTTTTAATGAAGCAGATACTTATAACTTTGCTAATAATTTTGCCAAAAATCAACAAAGTAAAATTCAAATTAAGAATCTTTATCATATGTTTTATAGTCTTTTTAAAGGCAAGACAATAATGGCTAATGTTCCAGTAAGATTTACTCCAAGCCAAATTTATCAAACTTATGCAGACAAGACAGGATTTGTTAGTAGATTATGA", "aa": "MSAFHLQNSSQEIIIDSDTMGKLTTGIDLNIFKGGKSNLEKLQEIVEQMDNETAIGNFIIKYGYEVKPISDGNHFNLIFKIKQLKYKGKDITNSFASKIFNEADTYNFANNFAKNQQSKIQIKNLYHMFYSLFKGKTIMANVPVRFTPSQIYQTYADKTGFVSRL", "aa_hexdigest": "18837d5f2cab88a1db467aa3e85a243a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18891.375399999983, "isoelectric_point": 9.260125923156735 }, "id": "DFOKDJOJIG_246", "locus": "GCA013367855_00208" }, { "type": "cds", "sequence": "contig_1", "start": 117472, "stop": 117621, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGGCTACAAAAAATATATCAACGACTTAACTTTTTATCACGGCGATGGTAATGAGTGGTGTGATCAAGATGGGTGCCAGGAAGAAACACATAGCAACGGCGAAATTACTCAAACTATTAAAAAATCTAATTATAGTATTAACTGA", "aa": "MYGYKKYINDLTFYHGDGNEWCDQDGCQEETHSNGEITQTIKKSNYSIN", "aa_hexdigest": "9ceab7927a4c10fe9c661039847e1820", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5740.112799999999, "isoelectric_point": 4.734767723083496 }, "id": "DFOKDJOJIG_247", "locus": "GCA013367855_00209" }, { "type": "cds", "sequence": "contig_1", "start": 117862, "stop": 118056, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EDE39", "UniRef:UniRef90_UPI001E2EDE39" ], "nt": "ATGAAGAAAATTAAAAAAACCTTATTGATAGCCGGAGGAATATTTAGTATTGCTTCGGCTTCTCTATTTTCTTTGAGTTTTTCTAGAAATGAACATTATTCAAGTTATAAATATGAAGAAATTGACAAAGACCAATATTCTTTTTTAAACAACAAAAATTTATTAAAAAGTTTTTTTACAAATTTGACATATTGA", "aa": "MKKIKKTLLIAGGIFSIASASLFSLSFSRNEHYSSYKYEEIDKDQYSFLNNKNLLKSFFTNLTY", "aa_hexdigest": "3c30da2aa9a547cf19278edfa2c4f5b6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 117762, "stop": 118056, "observations": { "insertion": [ 117795 ], "mutation": [ 117864 ], "stop": [ 117864 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2EDE39", "query_cov": 1.0, "subject_cov": 0.6530612244897959, "identity": 1.0, "score": 123.0, "evalue": 1.85e-35, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MKKIKKTLLIAGGIFSIASASLFSLSFSRNEHYSSYKYEEIDKDQYSFLNNKNLLKSFFTNLTYKKNSRQDSFIFNPKSYSGNPLYSIKDLLQTKMIT" }, "paralog": false, "identity": 0.9278350515463918, "score": 171.0, "evalue": 1.17e-56, "description": "Internal stop codon at 117864. Frameshift due to insertion around 117795. Nonsense mutation around 117864.", "psc": { "uniref90_id": "UniRef90_UPI001E2EDE39", "uniref50_id": "UniRef50_UPI001E2EDE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2EDE39", "UniRef:UniRef50_UPI001E2EDE39" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2EDE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EDE39" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7455.452299999998, "isoelectric_point": 9.477255439758302 }, "id": "DFOKDJOJIG_248", "locus": "GCA013367855_00210" }, { "type": "cds", "sequence": "contig_1", "start": 118043, "stop": 118360, "strand": "-", "frame": 3, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA66", "UniRef:UniRef90_A0A454CA66" ], "nt": "TTGCCCTTTGTTATTGCACTATTTGAACAAGCAAATAAAACCAATTTTATCGAGATTACTTGTTTTAATAAATTGGCAGAACATGTTATGAAATATGTTAAAAAAGGAGATTTATTAGAAGTTGTTGGCAAATTACACAATGCTAAATTTAGTTCAAAGAATAACGAAACTATTTATAAAACAGAAATAATTGCTAAAAATATTACTTTTTTGACAAAGTCAAAATCAAATATTCAACCCATTTCAAAAGAAACCAACTCAGAAAATAATGAAAAAGACATTTTTGGAATAGATTTTTTTGAAGATGAAGAAAATTAA", "aa": "MPFVIALFEQANKTNFIEITCFNKLAEHVMKYVKKGDLLEVVGKLHNAKFSSKNNETIYKTEIIAKNITFLTKSKSNIQPISKETNSENNEKDIFGIDFFEDEEN", "aa_hexdigest": "f16d7948cf267e4824cd45f63ab496fc", "start_type": "TTG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A454CA66", "query_cov": 1.0, "subject_cov": 0.8076923076923077, "identity": 0.99, "score": 201.0, "evalue": 6.25e-65, "valid": true, "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_A0A454CA66" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA66", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA66" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "DFOKDJOJIG_249", "locus": "GCA013367855_00211" }, { "type": "cds", "sequence": "contig_1", "start": 118637, "stop": 118801, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00138A25BD", "UniRef:UniRef90_UPI00138A25BD" ], "nt": "ATGTCTATTATTAATTTAAAACTTAATATGATTATTAATGCTTTATTTAAAGATAATGGAGATTTAGAAAAACAAATTGATAATCCCGGGGCTGATATATTAGCAGAATCTAAGTTCTTTGAACGCACTATAAGAGGTGCTGTTAATAAATTAACTAATGATTGA", "aa": "MSIINLKLNMIINALFKDNGDLEKQIDNPGADILAESKFFERTIRGAVNKLTND", "aa_hexdigest": "6426191af12777e3dadae3b37578ec71", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 118628, "stop": 118861, "observations": { "mutation": [ 118639 ], "stop": [ 118639 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00138A25BD", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 110.0, "evalue": 1.14e-30, "gene_start": 1, "gene_end": 54, "reference_start": 22, "reference_end": 75, "reference_sequence": "MSALSPYQLQINRRFRSMDIEMSIINLKLNMIINALFKDNGDLEKQIDNPGADILAESKFFERTIRGAVNKLTNDWTKNGK" }, "paralog": false, "identity": 0.987012987012987, "score": 149.0, "evalue": 2.08e-48, "description": "Internal stop codon at 118639. Nonsense mutation around 118639.", "psc": { "uniref90_id": "UniRef90_UPI00138A25BD", "uniref50_id": "UniRef50_UPI00138A25BD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00138A25BD", "UniRef:UniRef50_UPI00138A25BD" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00138A25BD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00138A25BD" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6063.910899999999, "isoelectric_point": 5.175554084777831 }, "id": "DFOKDJOJIG_250", "locus": "GCA013367855_00212" }, { "type": "cds", "sequence": "contig_1", "start": 119006, "stop": 119272, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAACTAATAAAAACGAATTAGATCAAGATTTTGATAATTTAGATAATAATGAAATTTTATCAAATGACAATCAAACTCAAAATGAAGATGACTTTATAGAAAATATTAATTTAACAAGTGATAATCAAATAAATAACATTATGAATTCTATCACTAGTTCCCAACCTAAAAAGCAAAAGGAAAAAAAGCAACAAAACAATTTTGAAGCACAATTTTCAGGACGATTTACTGATCCTGAAGATGTAGGAATGTTTTATCGTTGA", "aa": "MKTNKNELDQDFDNLDNNEILSNDNQTQNEDDFIENINLTSDNQINNIMNSITSSQPKKQKEKKQQNNFEAQFSGRFTDPEDVGMFYR", "aa_hexdigest": "99fc51e43057f7e6bac09ce1ee956bb5", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10338.974399999996, "isoelectric_point": 4.283920860290528 }, "id": "DFOKDJOJIG_251", "locus": "GCA013367855_00213" }, { "type": "cds", "sequence": "contig_1", "start": 119330, "stop": 119821, "strand": "-", "frame": 3, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F9", "UniRef:UniRef90_A0A454C9F9" ], "nt": "ATGTTAAAGAGAAAATATTTGCAAGAACATAACATTAAGTTAATGGATGCAAAACAAGTAGAAGATACTATTAGATTTTGTGCCGTCATCGTAGATGAAGCCCATTTATACATCGACCCTGATAATCCTGCCATTTTAAAATATATGGTTCAAATGACAAAAACGGTTAGAAAATTCAATGCAGGTATGATATTTACTACCCAAAATCCTGGTGATTTTACTATTAATAATGCCACAAGTGATGATGCATCTAGAATTATTCAAAACTGTCAATATTCTGTATTCTTTGGCCTAAAAGACCAAGATATTGACAAAGTGCAACAATTATTTAAAAACTCTAATCAATTATTACAAAGTGAAATTAGATATTTAGTAAATGCAAGATATGGAAAATGTTTATTTTCAATAAGTTCAAATAAAAGAATTAGAGTTGAAATTTATTACAACAAATTAGAACAAGAATTATTCTTCAAGTTCTTAAATAAAGAATGA", "aa": "MLKRKYLQEHNIKLMDAKQVEDTIRFCAVIVDEAHLYIDPDNPAILKYMVQMTKTVRKFNAGMIFTTQNPGDFTINNATSDDASRIIQNCQYSVFFGLKDQDIDKVQQLFKNSNQLLQSEIRYLVNARYGKCLFSISSNKRIRVEIYYNKLEQELFFKFLNKE", "aa_hexdigest": "dc888545ecc1107b7cb41b23ec2f7010", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 119330, "stop": 120121, "observations": { "mutation": [ 119836 ], "stop": [ 119836 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9F9", "query_cov": 1.0, "subject_cov": 0.5174603174603175, "identity": 0.9940000000000001, "score": 323.0, "evalue": 8.79e-110, "gene_start": 1, "gene_end": 163, "reference_start": 151, "reference_end": 313, "reference_sequence": "MSWLESFFKVLINIEDHKWILLQNVIKEFYEDRGIYKLASIEQLKAVEWPTISELIKYMSNYKTLHLNNPEAKRAILVELCESFSFLFENNGRYQNLFNGHTNIKLDNDFVVFNTQNLINGADNIGAKLGTMCLLNFVSEIVFNNWTHNEMLKRKYLQEHNIKLMDAKQVEDTIRFCAVIVDEAHLYIDPDNPAILKYMVQMTKTVRKFNAGMIFTTQNPGDFTVNNATSDDASRIIQNCQYSVFFGLKDQDIDKVQQLFKNSNQLLQSEIRYLVNARYGKCLFSISSNKRIRVEIYYNKLEQELFFKFLNKEWI" }, "paralog": false, "identity": 0.9923954372623575, "score": 526.0, "evalue": 3.22e-192, "description": "Internal stop codon at 119836. Nonsense mutation around 119836.", "psc": { "uniref90_id": "UniRef90_A0A454C9F9", "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A454C9F9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9F9", "UniRef:UniRef50_A0A454C9F9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9F9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F9" ], "product": "ATP-binding protein" } }, "id": "DFOKDJOJIG_252", "locus": "GCA013367855_00214" }, { "type": "cds", "sequence": "contig_1", "start": 119834, "stop": 120211, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTCAAAACGTAATAAAAGAATTTTATGAAGATAGAGGAATATATAAACTTGCTTCTATTGAGCAATTAAAGGCCGTAGAATGGCCAACAATAAGTGAGTTGATAAAATATATGTCCAACTATAAAACGCTTCATTTAAACAATCCTGAAGCAAAAAGAGCAATATTAGTTGAACTTTGTGAATCGTTTAGCTTTTTGTTTGAAAATAATGGAAGATATCAAAACTTATTTAATGGACATACTAATATAAAACTTGATAATGATTTTGTAGTATTTAATACGCAAAACTTAATAAATGGAGCGGATAATATCGGCGCTAAATTAGGAACAATGTGCTTATTAAACTTTGTTAGTGAAATAGTATTTAATAATTGA", "aa": "MLQNVIKEFYEDRGIYKLASIEQLKAVEWPTISELIKYMSNYKTLHLNNPEAKRAILVELCESFSFLFENNGRYQNLFNGHTNIKLDNDFVVFNTQNLINGADNIGAKLGTMCLLNFVSEIVFNN", "aa_hexdigest": "b7282ea7acc765500ab8863ad00101c8", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14430.339799999983, "isoelectric_point": 5.328053092956543 }, "id": "DFOKDJOJIG_253", "locus": "GCA013367855_00215" }, { "type": "cds", "sequence": "contig_1", "start": 120263, "stop": 120985, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01656.29", "PFAM:PF01935.23", "PFAM:PF10412.15" ], "nt": "ATGGCATCTATTGATACTACTAACGCAACAGGATCGGTGTCAGCAAACTTGGTTGGTTCTATTGATGAACAAGCGACAATGGCGATGATGTATCAAGTAAATAAAGATGAATACAAACTATTTGATAGTAATTTCTTCTTTGTATCAAAAGCCACTGACCTTGCTGAATTAAATAGACAGCATAGAATTGCTAGAAATATAGCAAGTAAAAAGAATATAAGCTTAAATGACTTAAAATATAGACAGCTTTTGGGTTTATCTGAGGCTTGCAATTATCCTTTTGAAAGATTAGAAAGAGAATATTATCAAATTACATCTATTAATTTAGCTTTGGGATGGCCTTTTGAAACCGAAAACCACAATGATTCAAATAATTTACTTCTAGGTTACTCAGAAGCCACAAGAAGTCCTCTAAGTTTTAGATTATTTGATATGTATCATTCAAATAGAACTAATTTTAATATGTTTATAAATGGTACTAGTGGAAAAGGTAAAACAACTTTTACTATGAAGTTATTAACCAGCATTTTAGCAGCAAATAATAAAGTTGTTGTTATTGACCCTCAAGGAGAATATAGAGAATTAGCTCAAAAACTTAATGGACAAATTATTGATTTGGGAGCAGGTAAAAACACCATAATCAATCCTCTTCAAATCCGTAATTCATTAAAGGATGCAACGGGTAATGATACATCTTCACTAATTAACAACCATTTGTCGTGA", "aa": "MASIDTTNATGSVSANLVGSIDEQATMAMMYQVNKDEYKLFDSNFFFVSKATDLAELNRQHRIARNIASKKNISLNDLKYRQLLGLSEACNYPFERLEREYYQITSINLALGWPFETENHNDSNNLLLGYSEATRSPLSFRLFDMYHSNRTNFNMFINGTSGKGKTTFTMKLLTSILAANNKVVVIDPQGEYRELAQKLNGQIIDLGAGKNTIINPLQIRNSLKDATGNDTSSLINNHLS", "aa_hexdigest": "cf0d37f42301b4c3b8415fe707399eb9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01656.29", "name": "CbiA", "length": 42, "aa_cov": 0.175, "hmm_cov": 1.0, "evalue": 2.9843715594778944e-07, "score": 26.120105743408203, "start": 155, "stop": 196 }, { "id": "PF01935.23", "name": "DUF87", "length": 68, "aa_cov": 0.26666666666666666, "hmm_cov": 0.9705882352941176, "evalue": 2.462838157755879e-10, "score": 36.4718132019043, "start": 157, "stop": 220 }, { "id": "PF10412.15", "name": "TrwB_AAD_bind", "length": 72, "aa_cov": 0.3, "hmm_cov": 0.875, "evalue": 1.5735730484289072e-10, "score": 36.0557746887207, "start": 149, "stop": 220 } ], "seq_stats": { "molecular_weight": 26953.050900000024, "isoelectric_point": 6.912837791442871 }, "id": "DFOKDJOJIG_254", "locus": "GCA013367855_00216" }, { "type": "cds", "sequence": "contig_1", "start": 121079, "stop": 121315, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATAAAGCAAGACTTCTAACATTTCTTAATAAACTTAATATGTTTGGTATTGATAGCCAAAACATACAAGAATATTGCAATGATTTAGATGCTAAATTTGGAGTTGAAAATTCATTAGATAACTTATTTATTTATAATCAAATTATTATTAAAGATAAATATTTACAAATCAATGATAATTACGTAAAAATCAATTGTTTAAACAAATTGCCATTTGAATTGTCTGATAAATGA", "aa": "MHKARLLTFLNKLNMFGIDSQNIQEYCNDLDAKFGVENSLDNLFIYNQIIIKDKYLQINDNYVKINCLNKLPFELSDK", "aa_hexdigest": "bb48840de9856d6d1d99c6de438389c9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9218.523299999993, "isoelectric_point": 5.646124076843263 }, "id": "DFOKDJOJIG_255", "locus": "GCA013367855_00217" }, { "type": "cds", "sequence": "contig_1", "start": 121652, "stop": 121789, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTAAATCCTTATTCATGTTTAGTTAATGAATATGTAGTCAAAAATAAAAATTCAAAAATAAGTTTTTATAATCCAAGAAATGATAAAGAAAACTATTTAGCGGTATTTAAACTAAATGGTCTTAATATTTGA", "aa": "MDLNPYSCLVNEYVVKNKNSKISFYNPRNDKENYLAVFKLNGLNI", "aa_hexdigest": "e4028ef62a76857cd24bf8df97f42e76", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5283.9867, "isoelectric_point": 8.852041053771973 }, "id": "DFOKDJOJIG_256", "locus": "GCA013367855_00218" }, { "type": "cds", "sequence": "contig_1", "start": 121881, "stop": 122411, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTATTCTTTCGATTTGGCATTATTAAAATAATTCCTAATAAAAAAGTAGGTATTCAAAATGCAAAGAAAATTAATTTCACATTTATTAATTGGCTAAAACCAAAACTAATAAAAAATGATAATAATAGAATTACAACAAATTCAATAAGATCTTTTGTTTTGAAAGTTTTTCAAATTTTACTACTTCTATGTTTTATTCTACTTGGCTGTAATTGCGTCATATTACTCCTTATACTTTGCTATTTTAGAAAACTTGCAACTTTATATGCTGCACCTACATTCGTGCCCTTTAATGCTGCATCCTTTACTGTTTTGTATGCTCCTGCCCCTGATCTTGCTCCAGACATAGCAAGTCTTGCAGATTGGCCACCAGAAACTTCACTTCCAACAAAAGCAATTGCAATTTCTGATAATTTTTTGAAAGCAAATGCAGAGCCAATTACCAAGAAACAAGTTGATAATAATGCTAAAACGGCTTTGATAGTAGTAAAGTTTGAGAATGAAGAAAAGGCTCCTATCATATAA", "aa": "MQLFFRFGIIKIIPNKKVGIQNAKKINFTFINWLKPKLIKNDNNRITTNSIRSFVLKVFQILLLLCFILLGCNCVILLLILCYFRKLATLYAAPTFVPFNAASFTVLYAPAPDLAPDIASLADWPPETSLPTKAIAISDNFLKANAEPITKKQVDNNAKTALIVVKFENEEKAPII", "aa_hexdigest": "602567f04168dc9dd7e9ce7b340f50b7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19755.3747, "isoelectric_point": 9.608642005920409 }, "id": "DFOKDJOJIG_257", "locus": "GCA013367855_00219" }, { "type": "cds", "sequence": "contig_1", "start": 123104, "stop": 123328, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E52DBAB", "UniRef:UniRef90_UPI001E52DBAB" ], "nt": "ATGAAATTTTTACAGGCGATAATAAATCCTTTAATAAATGTAATCGCAGCGCCTATTAGGCTTATGTGGTTTGTACCTCTTGCTGCTATTGGATTAATAGGATTTACTATATTTAATCTTTTGTTTTTCTTGTTTAAATTGTTTAGCCTCGATTTAAACAACTATTTAATGTTTAATTTATTACCAGGCCAAAGTTATAGCCAATTTTCTGTAGCAAGGCCTTGA", "aa": "MKFLQAIINPLINVIAAPIRLMWFVPLAAIGLIGFTIFNLLFFLFKLFSLDLNNYLMFNLLPGQSYSQFSVARP", "aa_hexdigest": "d1e23a59454a415dd491de6565c094cc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 123016, "stop": 123328, "observations": { "insertion": [ 123061 ], "mutation": [ 123073, 123106 ], "stop": [ 123073, 123106 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E52DBAB", "query_cov": 1.0, "subject_cov": 0.7184466019417476, "identity": 0.973, "score": 135.0, "evalue": 8.74e-40, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MGFLKAIINPLINVIAAPIRLMWFVPLAAIGLIGFTIFNLLFFLFKLFSLDLNNYLMFNLLPGQSYSQFSVARPWYQFLIISFILWLINFLCLLLGIQKNLMD" }, "paralog": false, "identity": 0.941747572815534, "score": 182.0, "evalue": 3.87e-61, "description": "Internal stop codon at 123073, 123106. Frameshift due to insertion around 123061. Nonsense mutation around 123073, 123106.", "psc": { "uniref90_id": "UniRef90_UPI001E52DBAB", "uniref50_id": "UniRef50_UPI001E52DBAB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E52DBAB", "UniRef:UniRef50_UPI001E52DBAB" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E52DBAB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E52DBAB" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8468.174799999993, "isoelectric_point": 9.991971015930176 }, "id": "DFOKDJOJIG_258", "locus": "GCA013367855_00220" }, { "type": "cds", "sequence": "contig_1", "start": 123573, "stop": 123692, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATCGATGATAAAAGCTTTAGCTTTTTAGGAAATTTTAGTGAAACTATAATGAGGTCTATTTTTATAGCCCTTATTCCAGTTGCAATTATTGTATTAATAGGAATGATATTGTGA", "aa": "MKIDDKSFSFLGNFSETIMRSIFIALIPVAIIVLIGMIL", "aa_hexdigest": "d18ca949acc3f7ed5bd5f985436aca2a", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4358.317699999999, "isoelectric_point": 5.876663017272949 }, "id": "DFOKDJOJIG_259", "locus": "GCA013367855_00221" }, { "type": "cds", "sequence": "contig_1", "start": 123912, "stop": 124088, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATTGAATTTAATAATTTAGAAACGCTTATGCTAGACAGAAATAAAAAAGATTCATATATTTATGACTATATCTTTAAATATATAAATGTCATTTTTAAAGCTAAATATAAAGACATTGATGAAATCAAAAAACCAAATGGATATTTAGATTTAAATAATAAAGAACATTGA", "aa": "MPIEFNNLETLMLDRNKKDSYIYDYIFKYINVIFKAKYKDIDEIKKPNGYLDLNNKEH", "aa_hexdigest": "7d05961910cb80544d854c663838b2c6", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7121.105200000002, "isoelectric_point": 6.546112632751465 }, "id": "DFOKDJOJIG_260", "locus": "GCA013367855_00222" }, { "type": "cds", "sequence": "contig_1", "start": 124092, "stop": 124199, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E44B7D1", "UniRef:UniRef90_UPI001E44B7D1" ], "nt": "ATGAAGAGTTTAACAATAGGTGACAAAATTGACTTTGATTACACATATAGATTTGCTTATAAATTTTTATATGAAAAATTAGTGCCTACAATGGTGGCAAACGGTTGA", "aa": "MKSLTIGDKIDFDYTYRFAYKFLYEKLVPTMVANG", "aa_hexdigest": "8e5b417499fbc7ff4464986ab040ae0f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 124044, "stop": 124199, "observations": { "mutation": [ 124094 ], "stop": [ 124094 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E44B7D1", "query_cov": 1.0, "subject_cov": 0.660377358490566, "identity": 0.971, "score": 72.8, "evalue": 2.16e-16, "gene_start": 1, "gene_end": 35, "reference_start": 1, "reference_end": 35, "reference_sequence": "MKSLTMGDKIDFDYTYRFAYKFLYEKLVPTMVANGWNMPGEFDVLEKNMLDEK" }, "paralog": false, "identity": 0.8627450980392157, "score": 89.4, "evalue": 1.01e-25, "description": "Internal stop codon at 124094. Nonsense mutation around 124094.", "psc": { "uniref90_id": "UniRef90_UPI001E44B7D1", "uniref50_id": "UniRef50_UPI001E44B7D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E44B7D1", "UniRef:UniRef50_UPI001E44B7D1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E44B7D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E44B7D1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4139.787800000002, "isoelectric_point": 8.032776832580566 }, "id": "DFOKDJOJIG_261", "locus": "GCA013367855_00223" }, { "type": "cds", "sequence": "contig_1", "start": 124398, "stop": 124580, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAATAAATTTCCACAACAAACTTATGATATGTATACTGATAAAAATGGTCTAGATAAAACAACTCCTACTCCTGCTTATAATAACTACAATCCAGTAGCATTTAATTTCTTAGTAATGGAAAGATTTGACTTATCAGAAAAATTAAATTTTAAAGTTTATAAGAATTTAATTCAATGA", "aa": "MRNKFPQQTYDMYTDKNGLDKTTPTPAYNNYNPVAFNFLVMERFDLSEKLNFKVYKNLIQ", "aa_hexdigest": "35ddc5bf27f61669d63858c8582cfd40", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7217.132799999999, "isoelectric_point": 9.003735160827638 }, "id": "DFOKDJOJIG_262", "locus": "GCA013367855_00224" }, { "type": "cds", "sequence": "contig_1", "start": 124644, "stop": 124754, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E37B992", "UniRef:UniRef90_UPI001E37B992" ], "nt": "ATGCCTTCTACTTTTATTAGAGACGAATTTAACCACCAATCAATTGAAGACATGGATGGATTTGTTAAAGAATTAATATCTGATTATGAAAATGCTATGTATGAAGGATGA", "aa": "MPSTFIRDEFNHQSIEDMDGFVKELISDYENAMYEG", "aa_hexdigest": "ca841bba6d4befa1bd0c027b5776d996", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 124523, "stop": 124781, "observations": { "insertion": [ 124604 ], "mutation": [ 124646 ], "stop": [ 124579, 124646 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E37B992", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 1.0, "score": 76.6, "evalue": 1.6e-17, "gene_start": 1, "gene_end": 36, "reference_start": 12, "reference_end": 47, "reference_sequence": "MNKNLFTHDSKMPSTFIRDEFNHQSIEDMDGFVKELISDYENAMYEGWTVENKANDWFVAPFHLDLILQWEINFHNKLMICILIKMV" }, "paralog": false, "identity": 0.9176470588235294, "score": 155.0, "evalue": 5.39e-51, "description": "Internal stop codon at 124579, 124646. Frameshift due to insertion around 124604. Nonsense mutation around 124646.", "psc": { "uniref90_id": "UniRef90_UPI001E37B992", "uniref50_id": "UniRef50_UPI001E37B992", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E37B992", "UniRef:UniRef50_UPI001E37B992" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E37B992", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E37B992" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4259.6153, "isoelectric_point": 4.0500284194946286 }, "id": "DFOKDJOJIG_263", "locus": "GCA013367855_00225" }, { "type": "cds", "sequence": "contig_1", "start": 124791, "stop": 124997, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGAAAGTGATGAAAACAAAGAAGGTATCAACCAAGGCGGTCGTTTTAAACCAAAAGATAATGAAAGTTATAATGAAGCCTTAATGAGATTTTTAAAAGAAATGGATGATTATTTAGATCCTGATATTGCATTTAAAGGTTTAACAATGGATGAAACTCATATAAATTATGTAACGGAAAATTTAGCTAAAGATCCTAAATGA", "aa": "MAESDENKEGINQGGRFKPKDNESYNEALMRFLKEMDDYLDPDIAFKGLTMDETHINYVTENLAKDPK", "aa_hexdigest": "e8234ce9e96894d500c75ddc58d5bb15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7872.6335999999965, "isoelectric_point": 4.491837882995605 }, "id": "DFOKDJOJIG_264", "locus": "GCA013367855_00226" }, { "type": "cds", "sequence": "contig_1", "start": 125169, "stop": 125426, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATAAAAATAATCCTAATCCTACCCCAACACCGGGTCCAGATTCGGGTTTAGTAAATCCAAATCCTTCAAACCCAACTCCTGCTCCTGCTCCAGTTCCAACCCCTGAAGTTAAACCAGAAGAAAAAGAAGGTTATGTTATTTATAAATCGACAAATGAAGATTATTTAAAAAAGAATAACACTTATTATTTTAGCAAATTGAGTTATAACCCCGTGGAATTGTCTGATGAGATTTATAATAGTCAAAAATGA", "aa": "MINKNNPNPTPTPGPDSGLVNPNPSNPTPAPAPVPTPEVKPEEKEGYVIYKSTNEDYLKKNNTYYFSKLSYNPVELSDEIYNSQK", "aa_hexdigest": "a91a8a19ef47d6e17cbb43d043924e66", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9484.356899999999, "isoelectric_point": 4.962578392028808 }, "id": "DFOKDJOJIG_265", "locus": "GCA013367855_00227" }, { "type": "cds", "sequence": "contig_1", "start": 126005, "stop": 126322, "strand": "-", "frame": 3, "gene": null, "product": "sugar-phosphate nucleotidyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313E05C", "UniRef:UniRef90_UPI001313E05C" ], "nt": "TTGGGCTATAAAGTAATATATTCGCATATTGATAATGGGCAAGATTTTTATAAATATGAGCCTATTGAAAATTATGATTTAATAATATCGAATCCTCCGTTTAGAAATAAAGCAAATATTATTAAAAGATTGCAAGAACTAAATAAACCGTTTGCTTTAATTTTTGGAGTTCAATGTTTTAATTCTGGCGGTTTTGTAAGCCAATTGCAAAAACTAAAAAATTTAGAGCTTGTGTTCTTAACAAAAAGAATTAAATTTCTTAAAAATTATAAACAAGACCTTAAAAACATTCCTCAGCCTACTTTTCATTCTCTATGA", "aa": "MGYKVIYSHIDNGQDFYKYEPIENYDLIISNPPFRNKANIIKRLQELNKPFALIFGVQCFNSGGFVSQLQKLKNLELVFLTKRIKFLKNYKQDLKNIPQPTFHSL", "aa_hexdigest": "3b68e2eb6f24447bacae676d47247993", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 125951, "stop": 126367, "observations": { "mutation": [ 126007 ], "stop": [ 126007 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001313E05C", "query_cov": 1.0, "subject_cov": 0.7553956834532374, "identity": 0.99, "score": 214.0, "evalue": 2.72e-70, "gene_start": 1, "gene_end": 105, "reference_start": 17, "reference_end": 121, "reference_sequence": "MPFDTQQSNFVIVLKSLGYKVIYSHIDNGQDFYKYEPIENYDLIISNPPFRNKANIIKRLQELNKPFALIFGVQCFNSGGFVSQLQKLKNLELVFLTKRIKFLKNYKQDLKNIPQPTFHSLWICSGITNKPLSILEGVK" }, "paralog": false, "identity": 0.9855072463768116, "score": 276.0, "evalue": 5.18e-97, "description": "Internal stop codon at 126007. Nonsense mutation around 126007.", "psc": { "uniref90_id": "UniRef90_UPI001313E05C", "product": "sugar-phosphate nucleotidyltransferase", "uniref50_id": "UniRef50_UPI001313E05C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001313E05C", "UniRef:UniRef50_UPI001313E05C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001313E05C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313E05C" ], "product": "sugar-phosphate nucleotidyltransferase" } }, "id": "DFOKDJOJIG_266", "locus": "GCA013367855_00228" }, { "type": "cds", "sequence": "contig_1", "start": 126477, "stop": 126587, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAACAATTATTCTTTTAATTATCATAATTAGACATTTGAAAAAACGCAATAAATTATTAGCAAATTCTAACCAAATTAATAGCGCTAAAGGAGTGATAAATAAATAA", "aa": "MITIILLIIIIRHLKKRNKLLANSNQINSAKGVINK", "aa_hexdigest": "eefd1c41d5c8ceca7425f556f70597b0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4096.028700000002, "isoelectric_point": 11.999967765808105 }, "id": "DFOKDJOJIG_267", "locus": "GCA013367855_00229" }, { "type": "cds", "sequence": "contig_1", "start": 126601, "stop": 126792, "strand": "-", "frame": 1, "gene": null, "product": "DUF1508 domain-containing protein", "db_xrefs": [ "RefSeq:WP_036439043.1", "SO:0001217", "UniParc:UPI000513025C", "UniRef:UniRef100_A0A454C8Y5", "UniRef:UniRef50_A0A9Q9F2Q3", "UniRef:UniRef90_A0A454C8Y5" ], "nt": "GTGTCAAAATACTTTTATAGATTAAGAGGGAGTCAATCTACAGGGAAGAATGCCAAAACATATTCGTTTGGCTCTGCGCAACAATACAATAAAGCATTAATAATGGCTCAAGATGCTATTTCAAAAAATCAATTTAAGGAGGTGCAAATAGAAAGAATACAAGTTATAAAAATTATTAAGCAAAATTCATAG", "aa": "MSKYFYRLRGSQSTGKNAKTYSFGSAQQYNKALIMAQDAISKNQFKEVQIERIQVIKIIKQNS", "aa_hexdigest": "bb61ae58264773743c7974dccf1b1a0d", "start_type": "GTG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000513025C", "ncbi_nrp_id": "WP_036439043.1", "uniref100_id": "UniRef100_A0A454C8Y5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513025C", "RefSeq:WP_036439043.1", "UniRef:UniRef100_A0A454C8Y5" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C8Y5", "uniref90_id": "UniRef90_A0A454C8Y5" }, "psc": { "uniref90_id": "UniRef90_A0A454C8Y5", "uniref50_id": "UniRef50_A0A9Q9F2Q3" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2Q3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2Q3" ], "product": "DUF1508 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_268", "locus": "GCA013367855_00230" }, { "type": "cds", "sequence": "contig_1", "start": 126966, "stop": 127301, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCAATCCGTGAAGGTCTAGAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGCTGGTTATGAAATGTCTGTAATCCGTGAAGGTCTAGCCCAAGGCCTTGATATATCTCAATATAACCAACTAGACCCTCAAGGCCACCCAATTTTCGTTGGTAGCCAAATGCACGAAATTCGTAAGGGTCTAGAACAAGGCCTTGATATATCTCAATATACCCAATTAAATCATAAAGGCCATCCAATCTATAATGCTTACCAAATGCACGAAATTCGCATTGGCCTACTATTTGATTTTTTAGGATCATGA", "aa": "MYAIREGLEQGLDISKYTQLDPNGHPIYAGYEMSVIREGLAQGLDISQYNQLDPQGHPIFVGSQMHEIRKGLEQGLDISQYTQLNHKGHPIYNAYQMHEIRIGLLFDFLGS", "aa_hexdigest": "41904fd1a9cbc9c37b395101b508c202", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12619.068699999993, "isoelectric_point": 5.332713890075683 }, "id": "DFOKDJOJIG_269", "locus": "GCA013367855_00231" }, { "type": "cds", "sequence": "contig_1", "start": 127308, "stop": 127619, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAATTTTCAATAGTGATCAAATGAATGAAATCCGCATTGGCCTAGAAGAAGGTCTAGACGTATCCAAATACATCCAATTATCCCCTCACGGTCGCCCAATCTATGATAGTTATCAAATGTATGCAATTCGTAAGGGTCTAAAACAAGACCTTGATATCTCTCAATATACCCAATTAAATCATAAAGGTCGCCCAATCTATGATGGTTATGAAATGTCTGTAATCCGTGAAGGTTTAAAAGCTGGCCTTGATGTCTCTCAATATAACCAACTAGACCCTCAAGGCCACCCAATTTTTGATTGA", "aa": "MKKKIFNSDQMNEIRIGLEEGLDVSKYIQLSPHGRPIYDSYQMYAIRKGLKQDLDISQYTQLNHKGRPIYDGYEMSVIREGLKAGLDVSQYNQLDPQGHPIFD", "aa_hexdigest": "126f5412d616ec80c3270f843a2a49d3", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11950.412999999993, "isoelectric_point": 6.2719213485717775 }, "id": "DFOKDJOJIG_270", "locus": "GCA013367855_00232" }, { "type": "cds", "sequence": "contig_1", "start": 127629, "stop": 127937, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175972201.1", "SO:0001217", "UniParc:UPI00158A229D", "UniRef:UniRef100_UPI00158A229D", "UniRef:UniRef50_A0A6A8PZR1", "UniRef:UniRef90_A0A6A8PZR1" ], "nt": "ATGCATATAGATGAATTTATTGATAAATATCGCAGGAATTTAAAACAATCGGGTTTTAATAAAGACAATATAGACTTCTTTTTATATGGTTTAGAACTAGGATTAAATATAAAAATAAAATCCAGTAGAAATTATTACAACTTTAAAAAATCTATACATTATTCTTCAAATAATTCTCATCCCTCTTCCCTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCGGAATCCCTATCAAAATCCCATTCATTAACAAAATAA", "aa": "MHIDEFIDKYRRNLKQSGFNKDNIDFFLYGLELGLNIKIKSSRNYYNFKKSIHYSSNNSHPSSLSDFDKTPTDLQDSKVINEYNKSSHTPSESLSKSHSLTK", "aa_hexdigest": "046a545343a2a85e9a188a55c5994240", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158A229D", "ncbi_nrp_id": "WP_175972201.1", "uniref100_id": "UniRef100_UPI00158A229D", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158A229D", "RefSeq:WP_175972201.1", "UniRef:UniRef100_UPI00158A229D" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158A229D", "uniref90_id": "UniRef90_A0A6A8PZR1" }, "psc": { "uniref90_id": "UniRef90_A0A6A8PZR1", "uniref50_id": "UniRef50_A0A6A8PZR1" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZR1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11882.013199999987, "isoelectric_point": 9.098890495300292 }, "id": "DFOKDJOJIG_271", "locus": "GCA013367855_00233" }, { "type": "cds", "sequence": "contig_1", "start": 127928, "stop": 128419, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAATCCGCATTGGCATAGAACAAGACCTTGATATATCTCAATATAACAAACTAGACCCTCAAGGCCATCCAATTTTCGTTGGTAGCCAAATGTCTGAAATTCGTTATGGCCTAAAACAAGGCCTTGATGTCTCTAAATATACCCAACTAGACTCTCAAGGCCACCCTATATTTGACAGCTTTCAAATGGGTGAAATCCGTAAGGGTCTAAAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGATTTTTGCCAAATGGAACAAATCCGTGAAGGCATAAAACAAGGCCTTGACGTATCTAAACCCACTCAATTAGTCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCCCACTCACAACCTAATCAAAATAAAACCTCAGAATCAAAAAATATAAATAGATGCATATAG", "aa": "MEQIRIGIEQDLDISQYNKLDPQGHPIFVGSQMSEIRYGLKQGLDVSKYTQLDSQGHPIFDSFQMGEIRKGLKQGLDISKYTQLDPNGHPIYDFCQMEQIREGIKQGLDVSKPTQLVSKSSLSDFDKTPTDLQDSKVINEYNKSHSQPNQNKTSESKNINRCI", "aa_hexdigest": "838b3a0f7ae0a3d7c528f71ab940495b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18550.55810000001, "isoelectric_point": 5.783390235900878 }, "id": "DFOKDJOJIG_272", "locus": "GCA013367855_00234" }, { "type": "cds", "sequence": "contig_1", "start": 128522, "stop": 128737, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAACAATTTTTGATAATGACCAAATGGAACAAATCTGCCTTGGTCTAGAACACGGCCTTGATATCTCTAAATATACTCAATTAAATCATAAAGGCCACCCTATATTTGATAGCTTCCAAATGAATGAAATCCGCGAAGGTCTAGAACACGGCCTTGATGTCACCCAATACACTCAACTAGACCCTCAAGGCCACCCCATCTATGATTGA", "aa": "MKKTIFDNDQMEQICLGLEHGLDISKYTQLNHKGHPIFDSFQMNEIREGLEHGLDVTQYTQLDPQGHPIYD", "aa_hexdigest": "010d3ad0acfd0d45522343f67a270a79", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8273.155999999995, "isoelectric_point": 4.917618751525878 }, "id": "DFOKDJOJIG_273", "locus": "GCA013367855_00235" }, { "type": "cds", "sequence": "contig_1", "start": 128943, "stop": 129425, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAATCCGCATTGGCCTAGAACATGGCCTTGACATCTCAATTTATGCAAAACAAAACAATTTTAATAGACCTATCTATGATTGTTTGCAAATGTCTGAAATCCGTGAAGGTCTAGAACAAGGCCTTGATATATCTCAATACAGCCAACTAGACCATAAAGGTCGCCCAATCTTTAATCGAGGTCAGATGTCTGAAATCCGTGAAGGTCTAGAACAAGGCCTTGATGTCTCACAATACACTCAACTAGACCTTCAAGGCCATCCAATATTCAATGGTTATCAAATGTATGCAATCCGTAAGGGTCTAGAACAAGGGCTTGACATCTCTCAATACACCCATTTGGCCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTCAAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCGGAATCCCAATCAAAATCCCATTCATTAACAAAATAA", "aa": "MEQIRIGLEHGLDISIYAKQNNFNRPIYDCLQMSEIREGLEQGLDISQYSQLDHKGRPIFNRGQMSEIREGLEQGLDVSQYTQLDLQGHPIFNGYQMYAIRKGLEQGLDISQYTHLASKSSLSDFDKTPTDLKDSKVINEYNKSSHTPSESQSKSHSLTK", "aa_hexdigest": "488f3e3c4bdf69e043f8da199ac57e64", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18269.103100000008, "isoelectric_point": 5.644987297058106 }, "id": "DFOKDJOJIG_274", "locus": "GCA013367855_00236" }, { "type": "cds", "sequence": "contig_1", "start": 129429, "stop": 129521, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGTAATCCGTGAAGGTCTAGAACAAGGCCTTGACATCTCACAATACACCCAACTAGACCTCAAAGGCCATCCAATCTATGATTTTTGA", "aa": "MYVIREGLEQGLDISQYTQLDLKGHPIYDF", "aa_hexdigest": "f392ce09b464ff1f92d3e9b1614ffcd7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3542.9643000000005, "isoelectric_point": 4.427268791198731 }, "id": "DFOKDJOJIG_275", "locus": "GCA013367855_00237" }, { "type": "cds", "sequence": "contig_1", "start": 129528, "stop": 129743, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAATTTTCAATCGTGATCAAATGGAACAAATCCACATTGGTTTAGTCCAAGGTCTTGATGTCACTCAATACACTCAACTAGACCCTCAAGGCCATCCAATATTCAATGATAATCAAATGACCGAAATCCGTAAGGGTCTAAAAAAAGGCCTTGATGTCTCTAAATATAATCAAATAGATCATGATGGATATCCGATATTTAATTGA", "aa": "MKKKIFNRDQMEQIHIGLVQGLDVTQYTQLDPQGHPIFNDNQMTEIRKGLKKGLDVSKYNQIDHDGYPIFN", "aa_hexdigest": "50c77cce3fde287027f172e412b761ee", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8302.352699999998, "isoelectric_point": 6.8166093826293945 }, "id": "DFOKDJOJIG_276", "locus": "GCA013367855_00238" }, { "type": "cds", "sequence": "contig_1", "start": 129753, "stop": 130052, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAATGAAGAATATGTTTATGTAAATCCCTATTTAGAAATAGAACCAATTAATATTAAAGAAATTAAAGACGATTACATAGAAGCATTAAAAACTTTTGATTTAGATGACCTTTTAGAATCAAGAGATGAAAAATCATTAATTAATTTAGATCCTAACAAATCTTCTAAATCATCTTTATCTGATTTTGACAAGACCCCAACCGATCTCAAAGATTCCAAAGTAATCAACGAATATAACAATTCATCCCATACCCCCTCTGATACCCAATCAAAATCCCATTCATTAACAAAATAA", "aa": "MSNEEYVYVNPYLEIEPINIKEIKDDYIEALKTFDLDDLLESRDEKSLINLDPNKSSKSSLSDFDKTPTDLKDSKVINEYNNSSHTPSDTQSKSHSLTK", "aa_hexdigest": "a6561f80382dc09bd9ef82e293825631", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11374.318299999984, "isoelectric_point": 4.538900566101075 }, "id": "DFOKDJOJIG_277", "locus": "GCA013367855_00239" }, { "type": "cds", "sequence": "contig_1", "start": 130514, "stop": 130702, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6", "UniRef:UniRef90_UPI00158CD6B8" ], "nt": "ATGGAAGCAGATTTATGGGCGTTTGACATTAATAACATTTATGAAATGCAAAACGATTTTTATGATATTGAATTCGTGGGCATTGACGCCTATGGTAGAGCCTTCATCATAAGCAGAGAAGATGCAGAAAAATATTATGCAAAGTACTATTTAAAACAAAACAAGTTAGTTGAAAAAGGAGATAAATAA", "aa": "MEADLWAFDINNIYEMQNDFYDIEFVGIDAYGRAFIISREDAEKYYAKYYLKQNKLVEKGDK", "aa_hexdigest": "2b989bd0783900700549016f6cdfd84f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 130514, "stop": 130915, "observations": { "mutation": [ 130738, 130849 ], "stop": [ 130738, 130849 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158CD6B8", "query_cov": 1.0, "subject_cov": 0.46616541353383456, "identity": 0.9840000000000001, "score": 128.0, "evalue": 7.17e-37, "gene_start": 1, "gene_end": 62, "reference_start": 72, "reference_end": 133, "reference_sequence": "MTKDKYCAFCGKKIVGMYVKNFWSDFCSREFRAKSEKLQEDLNYWKFHEPQDVYPLNDAWELADKELRKYGMEADLWAFDINNIYEMQNDFYDIEFVGIDAYGRAFVISREDAEKYYAKYYLKQNKLVEKGDK" }, "paralog": false, "identity": 0.9548872180451128, "score": 269.0, "evalue": 4.87e-95, "description": "Internal stop codon at 130738, 130849. Nonsense mutation around 130738, 130849.", "psc": { "uniref90_id": "UniRef90_UPI00158CD6B8", "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158CD6B8", "UniRef:UniRef50_A0A6A8PZW6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7473.2735999999995, "isoelectric_point": 4.464725685119628 }, "id": "DFOKDJOJIG_278", "locus": "GCA013367855_00240" }, { "type": "cds", "sequence": "contig_1", "start": 130924, "stop": 131121, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00145.23" ], "nt": "ATGATTATAGATAATAAACCATATTATTTAAGGGTTATTACACCATTAGAAGCAATGCTATTAATGGGCTATGATCAGCAAGATTATGAAAGAATTAAGAATTTCTGTGATAGTCAAATTTATAAGTTTGCTGGAAATTCTATTGTTGTTAATGTATTGCAAGAAATAATTAAACACATTTTTAGACACAAACAATAA", "aa": "MIIDNKPYYLRVITPLEAMLLMGYDQQDYERIKNFCDSQIYKFAGNSIVVNVLQEIIKHIFRHKQ", "aa_hexdigest": "60bdb35f264425eba9a2a021602d885f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00145.23", "name": "DNA_methylase", "length": 56, "aa_cov": 0.8615384615384616, "hmm_cov": 0.9464285714285714, "evalue": 9.34828096425361e-11, "score": 37.29964828491211, "start": 6, "stop": 61 } ], "seq_stats": { "molecular_weight": 7807.056499999999, "isoelectric_point": 7.875280570983887 }, "id": "DFOKDJOJIG_279", "locus": "GCA013367855_00241" }, { "type": "cds", "sequence": "contig_1", "start": 131560, "stop": 131925, "strand": "-", "frame": 1, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6N2ZW67", "UniRef:UniRef90_UPI0034A1F568" ], "nt": "ATGAATATAAATAAAATAAAAGTTGTTGAATTATTTGGCGGAATAGGCGCTATTAGAAAAGCTCTAATTAGAACCATCAAACCTCCTAATTTTGAAATTATTGATTATGTCGAAAGTGATAAAAAAACAGTAAATGCTTATAATGCAATATATAACGATTTTTACAAACCTTTAGATATAAGAGATTATTCACTTCCTAATAATGTTAATGTTGATTTATTGTTTCACGGATCTCCTTGTCAAGATTTTTCGTGTGCAGGTAAAAATATCGGAGGAAGTAAAGAAAGTAAAAGTCGAAGTAGTTTATTGTGGGAAACAATTAGAATCATTAAAGAAGCTCAAATAAAACCCAAAATTGTTATCTGA", "aa": "MNINKIKVVELFGGIGAIRKALIRTIKPPNFEIIDYVESDKKTVNAYNAIYNDFYKPLDIRDYSLPNNVNVDLLFHGSPCQDFSCAGKNIGGSKESKSRSSLLWETIRIIKEAQIKPKIVI", "aa_hexdigest": "eaf6fad3de3dcb6e7243cee8bcd6a62c", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0034A1F568", "query_cov": 0.8925619834710744, "subject_cov": 0.9230769230769231, "identity": 0.981, "score": 218.0, "evalue": 7.63e-72, "valid": true, "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_A0A6N2ZW67" }, "pscc": { "uniref50_id": "UniRef50_A0A6N2ZW67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6N2ZW67" ], "product": "DNA (cytosine-5-)-methyltransferase" }, "genes": [], "id": "DFOKDJOJIG_280", "locus": "GCA013367855_00242" }, { "type": "cds", "sequence": "contig_1", "start": 132204, "stop": 133172, "strand": "-", "frame": 2, "gene": null, "product": "type IV secretory system conjugative DNA transfer family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF33FFC", "UniRef:UniRef90_UPI000AF33FFC" ], "nt": "TTGTCAAAAACTCCTAAAGAAACATTTGGTGGAATATTAGCCGAAGCACAAAGTGTTTTAAATGTATTCAATAATGATGAAAATTTAAAGATGGTTTTAAGAGACACAGAAAGTTTCAATTTAGTTGATATAGTGTCCGATCCTAAACCTTTTGCTATATTTATACATTATCCTGACCATAAACCTACATCTCACTTTTTAGTAAGTATGTTGGTAGAACAAATTTATCAATCGTTAATTGAACAAGCAAACAAAAATGATAACCTAAAATTAGACAGAAAGGCTTTCTTTCTATTGGATGAATTCGGAAATTTACCTGTTGTTGTAAATTTTGATAATAAACTTACAATTTGTCGTTCAAGAAATATTTTCTTCATGCTGGTAGTACAAGATTACAATCAAATTAAGAAATATAACATATATGGAGAAGGAACAGCCCAAGTAATTAAAAACTCTTGCCAAGTAGTATATTTTTTAGCCTCTAATGATGAGCAAACATTAAACGATATTTCAAAAGCGCTCGGAACAAAAGAGGTTAAATCAAAAAGTTATACAACAAGTGATAACCATTCAAGTTCTACTGAAAGTGAAAAATCCAAACCTGTCATGGAAGTAGCGGAACTTAAATCTAAAAAACCTGATGATTTAATAATTATAAAAAATGGTTATAAACCTTTATTAATTAAAACCTTATTGGCTTATAAATATTTCCCAAATGATAATTATGTTTATACCAGAAACGAAAGTAAGCTACTAACTAATGCGGATTTTGAATTATTTGATATTAGAAGTTTGGAAAAACTTATTAAATTTGATAATTCTAAACAAAAAAAACAAAATAATAAAAACCCAAAAGTTTTAGATGATAACAATTTAACTTATTCAGAAGATGTTGAAGATCCTGCGGATTTATCCCAAGAACAAAAAATTCAAAGAATGAAGATGGTTTTTGGAAAATTAGATCTTTGA", "aa": "MSKTPKETFGGILAEAQSVLNVFNNDENLKMVLRDTESFNLVDIVSDPKPFAIFIHYPDHKPTSHFLVSMLVEQIYQSLIEQANKNDNLKLDRKAFFLLDEFGNLPVVVNFDNKLTICRSRNIFFMLVVQDYNQIKKYNIYGEGTAQVIKNSCQVVYFLASNDEQTLNDISKALGTKEVKSKSYTTSDNHSSSTESEKSKPVMEVAELKSKKPDDLIIIKNGYKPLLIKTLLAYKYFPNDNYVYTRNESKLLTNADFELFDIRSLEKLIKFDNSKQKKQNNKNPKVLDDNNLTYSEDVEDPADLSQEQKIQRMKMVFGKLDL", "aa_hexdigest": "4bef7560e91a67b66c035952680274f4", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 131937, "stop": 133403, "observations": { "mutation": [ 132053, 132104, 132206, 133202, 133250, 133277 ], "stop": [ 132053, 132104, 132206, 133202, 133250, 133277 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF33FFC", "query_cov": 1.0, "subject_cov": 0.6598360655737705, "identity": 0.972, "score": 602.0, "evalue": 3.4e-214, "gene_start": 1, "gene_end": 322, "reference_start": 78, "reference_end": 399, "reference_sequence": "QAKKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSHWENDKTISAWILYAKKRALDDNNYYWKKIYNNIEDLSKTPKETFGGILAEAQSVLNVFNNDENLKMVLRDTESFNLVDIVSDPKPFAIFIHYPDHKPTSHFLVSMLVEQIYQSLIEQANKNDNLKLDRKAFFLLDEFGNLPVVVNFDNKLTICRSRNIFFMLVVQDYNQIKKYNIYGEGTAQVIKNSCQVVYFLASNDEQTLNDISKALGTKEVKSKSYTTSDNHSSSTESEKSKPVMEVAELKSKKPDDLIIIKNGYKPLLIKTLLAYKYFPNDNYVYTRNESKLLTNADFELFDIRSLEKLIKLEKSKPKNQDNKNLKVLDDNNLTYSEDVEDPDDLSQEQKIQRMKMVFGKLDLWNNYINLKNDYKRTLIPHKKNQIKEQIEETLSLMWEQYVTNDLESDLLNILWSEAKERKIPNYHDLNVDILLYLLHPTEDISKLIEKSN" }, "paralog": false, "identity": 0.9713114754098361, "score": 926.0, "evalue": 0.0, "description": "Internal stop codon at 132053, 132104, 132206, 133202, 133250, 133277. Nonsense mutation around 132053, 132104, 132206, 133202, 133250, 133277.", "psc": { "uniref90_id": "UniRef90_UPI000AF33FFC", "product": "type IV secretory system conjugative DNA transfer family protein", "uniref50_id": "UniRef50_UPI000AF33FFC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF33FFC", "UniRef:UniRef50_UPI000AF33FFC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF33FFC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF33FFC" ], "product": "type IV secretory system conjugative DNA transfer family protein" } }, "id": "DFOKDJOJIG_281", "locus": "GCA013367855_00243" }, { "type": "cds", "sequence": "contig_1", "start": 133275, "stop": 133415, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E578F32", "UniRef:UniRef90_UPI001E578F32" ], "nt": "ATGTGGATTAGCCAAGCTAAAAAAACATTAATTGTTATTACTAATTTCTTATTGCTATTAGGTATTGATGATAAAAATATTATTCATAAAAATAATTTTAATTTCGAAACAATAGCTTCATTATTAAATACTTCTCACTGA", "aa": "MWISQAKKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSH", "aa_hexdigest": "b1a13d795929f5c842693543b06bcbaf", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 133227, "stop": 133505, "observations": { "insertion": [ 133427 ], "deletion": [ 133396 ], "mutation": [ 133249, 133276, 133439 ], "stop": [ 133249, 133276, 133439 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E578F32", "query_cov": 0.8478260869565217, "subject_cov": 0.40625, "identity": 1.0, "score": 77.4, "evalue": 1.56e-17, "gene_start": 8, "gene_end": 46, "reference_start": 37, "reference_end": 75, "reference_sequence": "MTFEERINKSLEVINNVIESLNWGKALMAICGLAKPKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSHRENDKTISAWILYAKKSTWWQ" }, "paralog": false, "identity": 0.9347826086956522, "score": 147.0, "evalue": 7.22e-48, "description": "Internal stop codon at 133249, 133276, 133439. Frameshift due to insertion around 133427. Frameshift due to deletion around 133396. Nonsense mutation around 133249, 133276, 133439.", "psc": { "uniref90_id": "UniRef90_UPI001E578F32", "uniref50_id": "UniRef50_UPI001E578F32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E578F32", "UniRef:UniRef50_UPI001E578F32" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E578F32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E578F32" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5284.135999999999, "isoelectric_point": 8.231081581115724 }, "id": "DFOKDJOJIG_282", "locus": "GCA013367855_00244" }, { "type": "cds", "sequence": "contig_1", "start": 133417, "stop": 133602, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCATTAACTGCCTTACCTCAATTTAAACTTTCAATAACATTATTTATAACTTCTAACGATTTATTAATTCTTTCTTCGAAAGTCAAATTCTTGAATTTTTCACCATCAACCGCTAAATCTCATGCATATGAAAGAGGGTTTCATTTTAGAGATAATTTAGGGTCAGAGAAATCAATTACATAA", "aa": "MPLTALPQFKLSITLFITSNDLLILSSKVKFLNFSPSTAKSHAYERGFHFRDNLGSEKSIT", "aa_hexdigest": "b7d7e4f1fd7336945595f2e5dd80b01d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6859.8555, "isoelectric_point": 9.696512413024902 }, "id": "DFOKDJOJIG_283", "locus": "GCA013367855_00245" }, { "type": "cds", "sequence": "contig_1", "start": 133560, "stop": 133727, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAATATTGAATTTATGGCACAACTTGATTATTCTAAAAAACCAAATTTAATAATTAGCGATCCAAAGAAAGAAATTATCAAATTTACAGGAGGCTTATTAACTGAAAACGATTACAAAATTTATGTAATTGATTTCTCTGACCCTAAATTATCTCTAAAATGA", "aa": "MPNIEFMAQLDYSKKPNLIISDPKKEIIKFTGGLLTENDYKIYVIDFSDPKLSLK", "aa_hexdigest": "9deac9918115d24a413a97288fdd0f20", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6364.380800000001, "isoelectric_point": 6.098903465270994 }, "id": "DFOKDJOJIG_284", "locus": "GCA013367855_00246" }, { "type": "cds", "sequence": "contig_1", "start": 134085, "stop": 134237, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAACAAGAAAAATTATCAAAATCAAAAATTATAGGAAAGACACTTCTTTCAATAATTGTTAGTACATTTTTGTTTTTTGTTCTAACTTTTATTATTAATGTTTTTATGGGCCAGAATAAAAATCCTGGACATTTTAAACTTTTTTGA", "aa": "MEEQEKLSKSKIIGKTLLSIIVSTFLFFVLTFIINVFMGQNKNPGHFKLF", "aa_hexdigest": "ba2bb57e9f1e8664bda2b7f300a10a08", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5777.879399999999, "isoelectric_point": 9.699671363830568 }, "id": "DFOKDJOJIG_285", "locus": "GCA013367855_00247" }, { "type": "cds", "sequence": "contig_1", "start": 134341, "stop": 134745, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313EAF8", "UniRef:UniRef90_UPI001313EAF8" ], "nt": "TTGTGCCAAGAATTCATTAAATCTTCAAAAAGTATTTTAGATGAAGTTTTAAACAAAAAAAATATTTCATATTCTCGTATTTCTGATGAAAATAAGAAAAATATTGAAACAATAAAAAAATTCTTATTAAATTATGACAATGATTATTCAAAGCTCTTTAATTCTTATCAAAAACAAATTAATGATTTAAATAATTTAAACATCGAAGATAAATTTCTAAAACAAAGAATTTCAAAAATTATAAATAATGAAATGGATGATTTTCAAAAACAAGTTGGTAATAAGATAATCAAAACCTTACTAAATGCCTATAAGCAAAATAATAATGCATTTACTAGCCGAAAGAATTTCGCTTCATTTTTCAATGAATTTCTAAAAATATTTAGCCTAAATAGATCGGAATGA", "aa": "MCQEFIKSSKSILDEVLNKKNISYSRISDENKKNIETIKKFLLNYDNDYSKLFNSYQKQINDLNNLNIEDKFLKQRISKIINNEMDDFQKQVGNKIIKTLLNAYKQNNNAFTSRKNFASFFNEFLKIFSLNRSE", "aa_hexdigest": "4b09a3798400806a690ffc25e6783ff9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 134245, "stop": 134886, "observations": { "mutation": [ 134343, 134823 ], "stop": [ 134343, 134823 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001313EAF8", "query_cov": 1.0, "subject_cov": 0.6291079812206573, "identity": 0.985, "score": 247.0, "evalue": 1.67e-81, "gene_start": 1, "gene_end": 134, "reference_start": 48, "reference_end": 181, "reference_sequence": "MGLSIKYDEDYQKINALTKDIWNTRKQIVGIIANNFANSNEIISKSNLCQEFIKSSKSILDEVLNKKNISYSRISDKNKKNIETIKKFLLNYDNDYSKLFNSYQKQINDLNNLNIEDKFLKQRISKIINNEMDDFQKQVGNKIIKTLLNAYKQNNNAFTSRKNFASFFNEFLKIFSLNRSEWALRNLAEREFLAKDLKELELVEEFYRSKLVH" }, "paralog": false, "identity": 0.9812206572769953, "score": 406.0, "evalue": 8.09e-147, "description": "Internal stop codon at 134343, 134823. Nonsense mutation around 134343, 134823.", "psc": { "uniref90_id": "UniRef90_UPI001313EAF8", "uniref50_id": "UniRef50_UPI001313EAF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001313EAF8", "UniRef:UniRef50_UPI001313EAF8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001313EAF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313EAF8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15970.975899999985, "isoelectric_point": 9.44772891998291 }, "id": "DFOKDJOJIG_286", "locus": "GCA013367855_00248" }, { "type": "cds", "sequence": "contig_1", "start": 135274, "stop": 135381, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E466C14", "UniRef:UniRef90_UPI001E466C14" ], "nt": "GTGGTAGATTACATTACAAGACCAGATAAATGCGTTGTATTTAAAGAAGATGAAAATAATTATGATGCTATGAAACTACTTCTTACTAAGGAAGATAAAATAAATTGA", "aa": "MVDYITRPDKCVVFKEDENNYDAMKLLLTKEDKIN", "aa_hexdigest": "4319b445e246145b5e30e86e9d399845", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 135213, "stop": 135381, "observations": { "insertion": [ 135231 ], "mutation": [ 135276 ], "stop": [ 135276 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E466C14", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 1.0, "score": 73.9, "evalue": 7.88e-17, "gene_start": 1, "gene_end": 35, "reference_start": 1, "reference_end": 35, "reference_sequence": "MVDYITRPDKCVVFKEDENNYDAMKLLLTKEDKINWYRQHLKINQNSKQSVFITY" }, "paralog": false, "identity": 0.9636363636363636, "score": 105.0, "evalue": 8.21e-32, "description": "Internal stop codon at 135276. Frameshift due to insertion around 135231. Nonsense mutation around 135276.", "psc": { "uniref90_id": "UniRef90_UPI001E466C14", "uniref50_id": "UniRef50_UPI001E466C14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E466C14", "UniRef:UniRef50_UPI001E466C14" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E466C14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E466C14" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4192.786300000001, "isoelectric_point": 4.716124534606934 }, "id": "DFOKDJOJIG_287", "locus": "GCA013367855_00249" }, { "type": "cds", "sequence": "contig_1", "start": 135791, "stop": 135955, "strand": "-", "frame": 3, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "RefSeq:WP_175972262.1", "SO:0001217", "UniParc:UPI00158EAE53", "UniRef:UniRef100_UPI00158C7902", "UniRef:UniRef50_A0A9Q9F2D4", "UniRef:UniRef90_A0A454C8Z1" ], "nt": "ATGAACAAAGCCATAGCCAATTATTTTGGAGTTGAATATTTGCAAATGTTTGGTATATTGCGTAGTCAAGAAAAAAAAGTTACAACATTAAAATTAGGAAATTTAAACATCAAATGTAATGTTTATAAATTTTCTACACAACGTTATTTGTTAATGCAAATTTAA", "aa": "MNKAIANYFGVEYLQMFGILRSQEKKVTTLKLGNLNIKCNVYKFSTQRYLLMQI", "aa_hexdigest": "cf685e3d1d55bbc71ebe10a3c2a6ac0f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158EAE53", "ncbi_nrp_id": "WP_175972262.1", "uniref100_id": "UniRef100_UPI00158C7902", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EAE53", "RefSeq:WP_175972262.1", "UniRef:UniRef100_UPI00158C7902" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C7902", "product": "MAGa4850 family ICE element protein", "uniref90_id": "UniRef90_A0A454C8Z1" }, "psc": { "uniref90_id": "UniRef90_A0A454C8Z1", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_A0A9Q9F2D4" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4" ] }, "genes": [], "id": "DFOKDJOJIG_288", "locus": "GCA013367855_00250" }, { "type": "cds", "sequence": "contig_1", "start": 135977, "stop": 136768, "strand": "-", "frame": 3, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4", "UniRef:UniRef90_A0A454C8Z1" ], "nt": "GTGGGGTTAATTCCAAATAAGGAAAAAAAGCAAGATCATAAAGGTCTTGCTTTTTTTCTATCATTCATAAAAAAAATAAGTTTAAAAAAACGAAGAGCACAACACAAGCACAGCTCTTCATACATCTACATAAATTATACCACGTGCTCTTCGTTTTTTTTAAAAAACTTAAAAATTTTTGAAAGGAATTTTATGGGTGCTTATAGTGAATTTATGGTAATGCAGCAAGAGGCTAAATTAAACAACGATTATAGCCTTGTGGATGCATATGAAAGAGATTTAAATTGGAGGAGAAATTATAGTAAAAAAATATATGACAAATTAAGCGACATAGCTATAAGCAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATGGTTATTAAATCCTTTAAAAATAAAAAAATCAAAATCATTGATCTAATAAATTTAGGGTTTAAAAAAACTTCTATAAAATCAGCTATAAAAAGACTTATTGAAATAAAAGTTTTAGATGGTGAGTTTTATAAAAAATATAAACTTCTAAAATTAAAAAAAATAGTACTAAAAAACCCTAAGGAAAATTACTGGATTTTAAATGGCTATGATGCCTTAAAAATCTTTCTTCTTAATGGTTTAAGTAATGCTATTTTATATGCAATAAATAGGCATAAATCAAAGCAACTAAATGCTAAAAAATTGCATTGTAAAATAAACTCAAGAAAAGTGATTTTACAAAATGCATATTATGCAAAATTTAACTGA", "aa": "MGLIPNKEKKQDHKGLAFFLSFIKKISLKKRRAQHKHSSSYIYINYTTCSSFFLKNLKIFERNFMGAYSEFMVMQQEAKLNNDYSLVDAYERDLNWRRNYSKKIYDKLSDIAISKILKSKIVISESYYQKLKTNKVLLEVFMVIKSFKNKKIKIIDLINLGFKKTSIKSAIKRLIEIKVLDGEFYKKYKLLKLKKIVLKNPKENYWILNGYDALKIFLLNGLSNAILYAINRHKSKQLNAKKLHCKINSRKVILQNAYYAKFN", "aa_hexdigest": "9888806883b49ae0a181b8923c514e9d", "start_type": "GTG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A454C8Z1", "query_cov": 1.0, "subject_cov": 0.8092307692307692, "identity": 1.0, "score": 499.0, "evalue": 3.98e-177, "valid": true, "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_A0A9Q9F2D4" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4" ] }, "genes": [], "id": "DFOKDJOJIG_289", "locus": "GCA013367855_00251" }, { "type": "cds", "sequence": "contig_1", "start": 136759, "stop": 137178, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_241862877.1", "SO:0001217", "UniParc:UPI001F521A35", "UniRef:UniRef100_UPI001F521A35", "UniRef:UniRef50_UPI001F521A35", "UniRef:UniRef90_UPI001F521A35" ], "nt": "ATGATTTGTTCTAGATTACTATTACCTTTGAATGATGTTGATGAATATAAATTAATTCCTCTTGTTAGAACAATAGAATTTACAATTTATGCAAAGGCTTCAAAAATAAAACACGATAATGAAGTACTTCTAACTTCAATATCAAATAATTTAAGTCAATATGACATTGATAATTTTCAAGGTTTATATTGCGATATAAACCAAGCATTTGTAGCTGATAATCAATTATTTGACGAAGAAACAGAGTACCAATTTAAATTCAGCAACTCAAATGATGAAGATAATTACCAAGCAAGTTATATTATTCAAAAACTAATAAAAAAACTATTAAATTTTGTCAATGATGAAGACTTTAATTATTGCTTTATTATTATGACAAAAATCCAAAATAATAGGAAGCGTAGAAAAATGTGGGGTTAA", "aa": "MICSRLLLPLNDVDEYKLIPLVRTIEFTIYAKASKIKHDNEVLLTSISNNLSQYDIDNFQGLYCDINQAFVADNQLFDEETEYQFKFSNSNDEDNYQASYIIQKLIKKLLNFVNDEDFNYCFIIMTKIQNNRKRRKMWG", "aa_hexdigest": "7e746700a4bd01d757406c584e81b1bc", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI001F521A35", "ncbi_nrp_id": "WP_241862877.1", "uniref100_id": "UniRef100_UPI001F521A35", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F521A35", "RefSeq:WP_241862877.1", "UniRef:UniRef100_UPI001F521A35" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F521A35", "uniref90_id": "UniRef90_UPI001F521A35" }, "psc": { "uniref90_id": "UniRef90_UPI001F521A35", "uniref50_id": "UniRef50_UPI001F521A35" }, "pscc": { "uniref50_id": "UniRef50_UPI001F521A35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F521A35" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16545.6164, "isoelectric_point": 4.983210945129394 }, "id": "DFOKDJOJIG_290", "locus": "GCA013367855_00252" }, { "type": "cds", "sequence": "contig_1", "start": 137356, "stop": 137523, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_162980102.1", "SO:0001217", "UniParc:UPI000305A2B6", "UniRef:UniRef100_UPI000305A2B6", "UniRef:UniRef50_UPI000305A2B6", "UniRef:UniRef90_UPI000305A2B6" ], "nt": "ATGAATGACAAAAATAATAAAGTAAGTGGAAACACACATACAAGACAACAAATGAATAATTATTCAAATCAAAATAATTCTAATAATTCAGCATACAGATCAAATTTAAACAATCATTCTAATCAATTGAACCCAAACAATAGCAACTACAAGGGCAACAAAAAATAA", "aa": "MNDKNNKVSGNTHTRQQMNNYSNQNNSNNSAYRSNLNNHSNQLNPNNSNYKGNKK", "aa_hexdigest": "4701f2ca3e730026670d7ff586fbd515", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000305A2B6", "ncbi_nrp_id": "WP_162980102.1", "uniref100_id": "UniRef100_UPI000305A2B6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000305A2B6", "RefSeq:WP_162980102.1", "UniRef:UniRef100_UPI000305A2B6" ] }, "ips": { "uniref100_id": "UniRef100_UPI000305A2B6", "uniref90_id": "UniRef90_UPI000305A2B6" }, "psc": { "uniref90_id": "UniRef90_UPI000305A2B6", "uniref50_id": "UniRef50_UPI000305A2B6" }, "pscc": { "uniref50_id": "UniRef50_UPI000305A2B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000305A2B6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6326.585400000004, "isoelectric_point": 10.214580345153806 }, "id": "DFOKDJOJIG_291", "locus": "GCA013367855_00253" }, { "type": "cds", "sequence": "contig_1", "start": 137695, "stop": 137871, "strand": "-", "frame": 1, "gene": null, "product": "putative dsRNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D72BC", "UniRef:UniRef90_UPI00215D72BC" ], "nt": "ATGTTAGATATTGAACATTTTTTTAAAGATGTTGATTGCAGAGAAAAAAGACCGGATAAATTTAAATTAGAAAATGCTATCAATACATTAAAAGAATTAGCAGAGCGTGGTGAATGCTCAATGCCAATATATCAAATATCAGAAAAACCCATAATTGATAATAACGGCAAACAATGA", "aa": "MLDIEHFFKDVDCREKRPDKFKLENAINTLKELAERGECSMPIYQISEKPIIDNNGKQ", "aa_hexdigest": "686ee4b118ebf1c2a58e18d103b559a8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 137533, "stop": 137961, "observations": { "mutation": [ 137655, 137694, 137697, 137907 ], "stop": [ 137655, 137694, 137697, 137907 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D72BC", "query_cov": 1.0, "subject_cov": 0.4084507042253521, "identity": 0.9309999999999999, "score": 114.0, "evalue": 3.34e-31, "gene_start": 1, "gene_end": 58, "reference_start": 31, "reference_end": 88, "reference_sequence": "MKADLFEAIIGAIAIDSGWDIKRLKNIIIKMLDIERFFRDVDCREKRPNKFKLENAINTLKELAERGECSMPIYQISEKPIIDDNGKQWWHCRCNVNFRNLQWMGLWTKYTEGYGTSKKLAKKMAAYNMLCDHYGLPNEYKN" }, "paralog": false, "identity": 0.9154929577464789, "score": 266.0, "evalue": 9.38e-94, "description": "Internal stop codon at 137655, 137694, 137697, 137907. Nonsense mutation around 137655, 137694, 137697, 137907.", "psc": { "uniref90_id": "UniRef90_UPI00215D72BC", "product": "putative dsRNA-binding protein", "uniref50_id": "UniRef50_UPI00215D72BC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D72BC", "UniRef:UniRef50_UPI00215D72BC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D72BC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D72BC" ], "product": "putative dsRNA-binding protein" } }, "id": "DFOKDJOJIG_292", "locus": "GCA013367855_00254" }, { "type": "cds", "sequence": "contig_1", "start": 137905, "stop": 138381, "strand": "-", "frame": 1, "gene": null, "product": "RNase III domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1G3X3E2" ], "nt": "ATGGGAATAGAAGAATGGCAAGAATATATTTATCAAATTGAATATACAATAGGATATTTCTTCAAAAATAAATTTTTACTTTATCAAGCATTTACTAGAAGTTCTTATTCAAATCAATATGGAGTTGAAAATAATGAAGTTCTTGAATTTATAGGAGATTCAGTTTTGAGCTTTTGCGTAGTTCAACTAATGGCAGAAGAATTTGGCTTTTTAAAATCGGCTTCGCAATATTACAACAAATATGAAGATCTTGAAGAATATTGCCTTATAGCCAATAAAAATGAATCCGATTTTACTGATTTAAAAAATCAAATCGTAAGCAATAAAACACTAGCTAAAAAAATTAATAAATTAGGTTTTGCTAAGTATTTAGTTTTAGGAGATAATGATAAAAGAAATAATGTTGCTAATCAAGATAGTGTGAAAGCTGATTTATTTGAAGCTATAATTGGTGCAATAGCAATTGACAGTGGTTGA", "aa": "MGIEEWQEYIYQIEYTIGYFFKNKFLLYQAFTRSSYSNQYGVENNEVLEFIGDSVLSFCVVQLMAEEFGFLKSASQYYNKYEDLEEYCLIANKNESDFTDLKNQIVSNKTLAKKINKLGFAKYLVLGDNDKRNNVANQDSVKADLFEAIIGAIAIDSG", "aa_hexdigest": "504609435cd77ff20038208487c1736f", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A2K9A1I8", "query_cov": 0.9810126582278481, "subject_cov": 0.8908045977011494, "identity": 0.535, "score": 162.0, "evalue": 3.4e-48, "valid": false, "product": "RNase III domain-containing protein", "uniref50_id": "UniRef50_A0A1G3X3E2" }, "pscc": { "uniref50_id": "UniRef50_A0A1G3X3E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1G3X3E2" ], "product": "RNase III domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_293", "locus": "GCA013367855_00255" }, { "type": "cds", "sequence": "contig_1", "start": 138698, "stop": 138985, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTAGCGTTTTATAATAATTTAAATAATATAATATTTATGTATTTAGGAGAAATTATGTCAAACGAAGAAAAAATTTATGTTTTTAGTTATGGAACTATTCAAGATCCACAATTTTATAAGGAATTATTACCAAACTCAAAGCCAATGCCTGCAATATTAAACGGTTATGCAAAATGTGTTGATGAAACAATGTATTTTTTATTAAAAAAAGATTTGTCAAGTCAAGTTAAGGGAAGCGTATTTGAAATTAATAAAGAAGAATTGTTTTTAATTGATAGATGA", "aa": "MILAFYNNLNNIIFMYLGEIMSNEEKIYVFSYGTIQDPQFYKELLPNSKPMPAILNGYAKCVDETMYFLLKKDLSSQVKGSVFEINKEELFLIDR", "aa_hexdigest": "abf9f013c78316a410fd5d97041bd2d3", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11135.848599999996, "isoelectric_point": 4.823322868347168 }, "id": "DFOKDJOJIG_294", "locus": "GCA013367855_00256" }, { "type": "cds", "sequence": "contig_1", "start": 139112, "stop": 139570, "strand": "+", "frame": 2, "gene": null, "product": "Putative gamma-glutamylcyclotransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2K9YSY5", "UniRef:UniRef90_A0A2K9YSY5" ], "nt": "ATGGGATTTTCTAGAAATCCTAATGCCAATGAAAATAATTTAAATGCTTTTATCGAAATGGAAAAGGCAATAAAAGATTTTCCATTAACTGACTATATATTTTTATATGATATAAACGAACAAGAATTTGAAGAAATTAATAAATTAACTCACCCTTATGCTGCTTTAATTATTGATGATAAGGAAAATAGAAATTATGTTGCAATTCACGGATCAATTTTTGCAATTAAAGAAGACGGTAAAATGTATGCAGCCTTAACTTCGTTTAGCCAAAAATCAAATTTAAATAGTATTTTTTATTATCAAGCATTTAATGAAAAGTTATTAAATAGCAAACCAGAAATAACGTTAAAATCGTTATATGATAATACAAATATAGATTTTTTAATTAATAAAAAACCTGTTTATTATTTAAGCTCAAGAGAAGACAAAACAATCAATGAAACTCAAGTTGGTTGA", "aa": "MGFSRNPNANENNLNAFIEMEKAIKDFPLTDYIFLYDINEQEFEEINKLTHPYAALIIDDKENRNYVAIHGSIFAIKEDGKMYAALTSFSQKSNLNSIFYYQAFNEKLLNSKPEITLKSLYDNTNIDFLINKKPVYYLSSREDKTINETQVG", "aa_hexdigest": "2bb34cbae2fa7cabb3d54e4c608c0fbd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 138812, "stop": 139684, "observations": { "mutation": [ 138983, 139568 ], "stop": [ 138983, 139568 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2K9YSY5", "query_cov": 1.0, "subject_cov": 0.45645645645645644, "identity": 1.0, "score": 303.0, "evalue": 8.1e-102, "gene_start": 1, "gene_end": 152, "reference_start": 144, "reference_end": 295, "reference_sequence": "QYEFYLILAFYNNLNNIIFMYLGEIMSNEEKIYVFSYGTIQDPQFYKELLPNSKQMPAILNGYAKCVDETMYFLLKKDLSSQVKGSVFEISKEELFLIDRWELFPQYQRFQVNVLLTETNEILENVYVYTKLEVGKYYLATDDMGFSRNPNANENNLNAFIEMEKAIKDFPLTDYIFLYDINEQEFEEINKLTHPYAALIIDDKENRNYVAIHGSIFAIKEDGKMYAALTSFSQKSNLNSIFYYQAFNEKLLNSKPEITLKSLYDNTNIDFLINKKPVYYLSSREDKTINETQVGWYENKAFELVEKDFDIDPFIRFNKMLKAFFDTKQKNDK" }, "paralog": false, "identity": 0.9862068965517241, "score": 571.0, "evalue": 1.25e-209, "description": "Internal stop codon at 138983, 139568. Nonsense mutation around 138983, 139568.", "psc": { "uniref90_id": "UniRef90_A0A2K9YSY5", "product": "Putative gamma-glutamylcyclotransferase", "uniref50_id": "UniRef50_A0A2K9YSY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2K9YSY5", "UniRef:UniRef50_A0A2K9YSY5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2K9YSY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2K9YSY5" ], "product": "Putative gamma-glutamylcyclotransferase" } }, "id": "DFOKDJOJIG_295", "locus": "GCA013367855_00257" }, { "type": "cds", "sequence": "contig_1", "start": 139791, "stop": 141380, "strand": "+", "frame": 3, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "COG:COG0322", "COG:L", "GO:0006289", "GO:0009380", "GO:0009381", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_A0A2Z5IPW9", "UniRef:UniRef90_D1J7M2" ], "nt": "ATGTTGCAATACTTTGATATAAAAATGCAAAATTCTTTTAAAACAGAAAAAATGAGGCAAGAGATTGCCGATTTTTCTACAATCATTTTAAGCTCGGAACGTGAAGCATTTATTGAAGAAAGAAAATTGATTGATTTTTACAAACCATTTTACAATGTTCTTTTTCCAACTAGAGATAGTTTCCCTTATATAAGAGTTAAATTACTAAGTACTAATAAATTAATAATTGATATAAAAAATCATTATAAAAAAGAACCAAATTCTATATATTATGGTCCTATTCCCGACAACAAAAATTTCAAACCATTAATTAGATATTTGAATCATTTACTACTTTCTAAGGATGGAATCATAATTGAAAAACAAAGTCTTGAATTTGCAAAGCAAAGATTTGAACAAGCCAAATCAATCATGAAATTTGGTCCTGATTTTAAAAGAAATTTAAAACAAAAAATCAATGAGGCAATTGAGGCTAACTTGTTTGAACAGGCAAGATTTTATGACCAAATTTTAGATTTATTAAATTACAATAAACAAAGTCAAAATATTTTTATAAAAACTATCAAAGATATTGATGTCTTTGGTTTTTATCAACATCAAAACTATATGATGGTTAATGTTATGATTTATAAAAACGGATCATTAGTAAATTCTTACGACTATATTTTTGAAATAATATTGAATGAACATGAATTTGTTAATCAATTTATTGATGAATTTTATGTTAATAATCAATTACCGGATCAAATAATAATACCTTTAAAATATTATGAATTAGGTGGATTTGATTATAATGACAAAGTAAGCGCGCCATCGAATAAATTAAATAAAAACTTGATCGATATTGCTATTGAGAATGCATCAAATAATATTGAACAAAAAATAAAAAATTATTTGAGTAAGCAAACAAAAGACAATGAAATTTTCAATAAATTATGTTCATATTTGAATGTTTATGCAGAGAGAATTGTTTTATTTGACAATTCATTTTTATCAAATACTAACGAAGTAATAGGGGCGGCTTGCTATTTTAAAAATGGCCAAGCTTTTAAAAAATTCTACCGTTTCTATAATTTAAAATTGAATTTATTAAGAAATGCAGATGTTGAATATATGCAACAAACTTCCTTTAATTATTTAAAAGATTTGGGAGATCAAGTTGATGTTGTTATTGTTGATGGTTCAATTGGTCAAATAAATGAAGTTAAAAAATCAATGGCATTTTTAAATTTAAATATTCCTCTATTCGGGTTGGTTAAAAATAGTAAACATGAAACTCAAAAATTAATTGATTCAAACAACAACGAAATTAAAATAGATGACCAAGATGTATTTAATTTGCTTTCAAGAATGCAAACAGAGGTTGATAGGTTTGCAAAATATAGATATAACCAAAAACAAAGAAAATCAATGTTATCTAATCCTCTATTAAAAATAAAAGGTATTGGATTTAAGACAATAGAAAAATTAATTAATTATTTTGGCAGTTATAAAAAAATTCAGCAAGCTTCTTTTGATGAAATATCAAAGGTGGCAAACAAAAAAGTTGCTAAGCTAATTTGCGAAGATAAAGGCGAATCGTTTTATTAA", "aa": "MLQYFDIKMQNSFKTEKMRQEIADFSTIILSSEREAFIEERKLIDFYKPFYNVLFPTRDSFPYIRVKLLSTNKLIIDIKNHYKKEPNSIYYGPIPDNKNFKPLIRYLNHLLLSKDGIIIEKQSLEFAKQRFEQAKSIMKFGPDFKRNLKQKINEAIEANLFEQARFYDQILDLLNYNKQSQNIFIKTIKDIDVFGFYQHQNYMMVNVMIYKNGSLVNSYDYIFEIILNEHEFVNQFIDEFYVNNQLPDQIIIPLKYYELGGFDYNDKVSAPSNKLNKNLIDIAIENASNNIEQKIKNYLSKQTKDNEIFNKLCSYLNVYAERIVLFDNSFLSNTNEVIGAACYFKNGQAFKKFYRFYNLKLNLLRNADVEYMQQTSFNYLKDLGDQVDVVIVDGSIGQINEVKKSMAFLNLNIPLFGLVKNSKHETQKLIDSNNNEIKIDDQDVFNLLSRMQTEVDRFAKYRYNQKQRKSMLSNPLLKIKGIGFKTIEKLINYFGSYKKIQQASFDEISKVANKKVAKLICEDKGESFY", "aa_hexdigest": "60389cc5f8dfdfdf27d9d68b6bca1301", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M2", "query_cov": 1.0, "subject_cov": 0.921602787456446, "identity": 0.987, "score": 1011.0, "evalue": 0.0, "valid": true, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_A0A2Z5IPW9", "cog_id": "COG0322", "cog_category": "L", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0006289", "GO:0009380", "GO:0009381" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z5IPW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z5IPW9" ], "product": "Excinuclease cho" }, "genes": [ "uvrC" ], "id": "DFOKDJOJIG_296", "locus": "GCA013367855_00258" }, { "type": "tRNA", "sequence": "contig_1", "start": 141445, "stop": 141520, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 76.3, "nt": "GGTCGTGTAGCTCAGGGGATAGAGTACGTGCCTTCTAAGCATGTGGTCGCAGGTTCGAATCCTGCCACGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 141478, 141480 ], "id": "DFOKDJOJIG_4", "locus": "GCA013367855_00259" }, { "type": "tRNA", "sequence": "contig_1", "start": 141590, "stop": 141663, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 71.2, "nt": "GCGAGTGTAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGAATACGTGGGTCCGATTCCCATCACTTGCACCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 141622, 141624 ], "id": "DFOKDJOJIG_5", "locus": "GCA013367855_00260" }, { "type": "tRNA", "sequence": "contig_1", "start": 141666, "stop": 141739, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 70.8, "nt": "GCGGATGTAGTTCAATGGTAGAACATCAGGTTGCCAATCTGAATACGTGGGTCCGATTCCCATCATCCGCACCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 141698, 141700 ], "id": "DFOKDJOJIG_6", "locus": "GCA013367855_00261" }, { "type": "cds", "sequence": "contig_1", "start": 142184, "stop": 142600, "strand": "+", "frame": 2, "gene": null, "product": "FMN-dependent NADH-azoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W7GCQ1" ], "nt": "ATGGCAAAAGTATTAATAATATATGGTTCAGTTATACCAAGAGAAAATTCAATTTCAACAAAAATGTTAGATCTTTTTGTTGATGAATATAAAAGAAATAATATTAATGATGAAATAAAAATTCTTGATTTAAATAATGAAGATATGTATTCATTAACTGACAAGAATTTTAAATTGTTTTATAGTGAAGAAAACTCTGACAAATATATAAATTTATTGAAGTCTGTTGATAAAGTTGTTATGAATGCGCCGATGATAAACTTCAATATTCCAACTATAATCAAGGCATTTTTTGACCGTATTGCAGTTGCAAATAAAACATTTTCATATAAATACGCAAGCAAAGGTTCTTCAATTGGTTTATTGAATAATTTAAAAGTTCAAATAATTGCAACGCAAGGAGCTCCGTTAGGTTGA", "aa": "MAKVLIIYGSVIPRENSISTKMLDLFVDEYKRNNINDEIKILDLNNEDMYSLTDKNFKLFYSEENSDKYINLLKSVDKVVMNAPMINFNIPTIIKAFFDRIAVANKTFSYKYASKGSSIGLLNNLKVQIIATQGAPLG", "aa_hexdigest": "192337f9037dd954a8548cfbf08c4d5f", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A2W7GCQ1", "query_cov": 0.8478260869565217, "subject_cov": 0.9212598425196851, "identity": 0.556, "score": 120.0, "evalue": 1.34e-32, "valid": false, "product": "FMN-dependent NADH-azoreductase", "uniref50_id": "UniRef50_A0A2W7GCQ1" }, "pscc": { "uniref50_id": "UniRef50_A0A2W7GCQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W7GCQ1" ], "product": "FMN-dependent NADH-azoreductase" }, "genes": [], "id": "DFOKDJOJIG_297", "locus": "GCA013367855_00262" }, { "type": "cds", "sequence": "contig_1", "start": 142880, "stop": 143302, "strand": "+", "frame": 2, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02867", "SO:0001217", "UniRef:UniRef50_B3PM28", "UniRef:UniRef90_D1J7M4" ], "nt": "ATGGCTAAAAAAGTAGTCAAGAAAGCAAAATTACAATTTGCTGCCGGCCAAGCAAAACCAGGCCCAGCATTGGCTGGTGTTGGTATTAACATGCCAGAATTTACAAAGGCATTTAATGACAAAACAAGAGAAAGAGGAAATGAACCAGTTCCTGTTTTAATTACTGTTTATGAAGATAAATCATTTGAATTTAAATTATTTACTTCTCCAACATCATACAAGTTAATCGAAGCTGCCAAAATTAAAAAAGGTAGCGGAGTTGCAAACAAAGAAAAAGTTGGTTCTATAACAATTGATCAATTAAGAGCAATTGCAGAATACAAATTACCTGATTTAAATACAACTAAAATTGATTCAGCAATGAAACAAGTAGCTGGTACTGCAAAGAATATGGGAATTACAGTTGAAGGTTATGACGAATGA", "aa": "MAKKVVKKAKLQFAAGQAKPGPALAGVGINMPEFTKAFNDKTRERGNEPVPVLITVYEDKSFEFKLFTSPTSYKLIEAAKIKKGSGVANKEKVGSITIDQLRAIAEYKLPDLNTTKIDSAMKQVAGTAKNMGITVEGYDE", "aa_hexdigest": "c988e0254890aaae286c794b34012d8d", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7M4", "query_cov": 1.0, "subject_cov": 0.9523809523809523, "identity": 1.0, "score": 271.0, "evalue": 8.2e-92, "valid": true, "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_B3PM28", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM28" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "DFOKDJOJIG_298", "locus": "GCA013367855_00263" }, { "type": "cds", "sequence": "contig_1", "start": 143323, "stop": 144015, "strand": "+", "frame": 1, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "COG:COG0081", "COG:J", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02863", "RefSeq:WP_114095899.1", "SO:0001217", "UniParc:UPI000DEDA28F", "UniRef:UniRef100_A0A6A8PZQ7", "UniRef:UniRef50_Q4A5E1", "UniRef:UniRef90_D1J7M5" ], "nt": "ATGGCTAAAAGAATTACAAAAAACATTAAAAATGTTAAAAGCTTAGTTAATAAAAAAGACGTATTCCCTCTTGCAGATGCTATTGAATTAGCAAAAAAAGCTTCATTTGCAAAATTTGATGAATCTGTTGATATTGCAATTAGATTAAATCTTGATACAAGAAAATCAGATCAACAATTACGTGGATCAGTTGTACTTCCACACGGTACAGGAAAATCAGTTAAAGTTTTAGTAGCAACTGACGAAGTTTCAGCCCAAAAAGCTTCATTAGAAGCTGGAGCAGATTTTGTTTACAGTTCAGCTGAATTACCAGAAGTATTAAATCAAGATAAATACGATTTTGATGTAATAGTTGCCGATCCTAAAATGATGTTAGTTTTAGGAAAATTTGGTAAAAAATTAGGACCTAAAGGTTTAATGCCAAACCCAAAAACAGGAACAGTTACAATCAATCCTGCTAAAGCTGTTGAAGAACTTAAAAAAGGTAAAGCAAATTATCGTGCTGATAAGGGTGGAATTGTTCACGCCTCATTTGGTAAGAAATCAATGGAAACAAATGTTCTAGCAGAAAATGCTGATACTATTATTCAAACAATTAAGAGATTAAAACCTCAAACAGTTAAAGGTACATATGTTTTAAATATTACCGTTTCAACTTCAATGGGTCAATCTGTTAAAGTAAAAATTGATTAA", "aa": "MAKRITKNIKNVKSLVNKKDVFPLADAIELAKKASFAKFDESVDIAIRLNLDTRKSDQQLRGSVVLPHGTGKSVKVLVATDEVSAQKASLEAGADFVYSSAELPEVLNQDKYDFDVIVADPKMMLVLGKFGKKLGPKGLMPNPKTGTVTINPAKAVEELKKGKANYRADKGGIVHASFGKKSMETNVLAENADTIIQTIKRLKPQTVKGTYVLNITVSTSMGQSVKVKID", "aa_hexdigest": "c5c242ad0634006481924b8fe763ecd6", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI000DEDA28F", "ncbi_nrp_id": "WP_114095899.1", "uniref100_id": "UniRef100_A0A6A8PZQ7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DEDA28F", "RefSeq:WP_114095899.1", "UniRef:UniRef100_A0A6A8PZQ7" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PZQ7", "uniref90_id": "UniRef90_D1J7M5" }, "psc": { "uniref90_id": "UniRef90_D1J7M5", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_Q4A5E1", "cog_id": "COG0081", "cog_category": "J", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5E1" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [ "rplA" ], "id": "DFOKDJOJIG_299", "locus": "GCA013367855_00264" }, { "type": "cds", "sequence": "contig_1", "start": 144126, "stop": 144518, "strand": "-", "frame": 2, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "nt": "ATGAATAAGAGATTTGGCAACAAAGTTCAACTAGTTGGAGATGACTTAATTGTAACAAATCCAAAATACATTCAAATGGCTATTGACAAGCATGCAATCAATGCATCTTTAATAAAGATAAATCAAATAGGTTCATTAACTGAAACATTTAAGGCAATAACAATGTCACAAAAAGCTGGCTTTGTAGCAATAATTTCTCACCGTTCAGGTGAAACTGAAGATACATTTATTGCAGATTTGGCAGTAGCATTAAATACTGGAGAAATCAAAACCGGCTCTCTTTCAAGAACAGATAGAATTGCTAAATACAATCGCTTGTTAAAAATAGAATCAGAATTAGGCAATGAAGCTAAATTCGAAGGCAAAAACTCATTTTATAATCTAAAAAATTAA", "aa": "MNKRFGNKVQLVGDDLIVTNPKYIQMAIDKHAINASLIKINQIGSLTETFKAITMSQKAGFVAIISHRSGETEDTFIADLAVALNTGEIKTGSLSRTDRIAKYNRLLKIESELGNEAKFEGKNSFYNLKN", "aa_hexdigest": "f9554e5da558142c1aa6a8c873f363d9", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A139MRN9", "query_cov": 0.9923076923076923, "subject_cov": 0.8376623376623377, "identity": 0.636, "score": 169.0, "evalue": 8.08e-52, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A139PDG5" }, "pscc": { "uniref50_id": "UniRef50_A0A139PDG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "product": "Enolase" }, "genes": [], "id": "DFOKDJOJIG_300", "locus": "GCA013367855_00265" }, { "type": "cds", "sequence": "contig_1", "start": 144534, "stop": 145301, "strand": "-", "frame": 2, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "COG:COG0148", "COG:G", "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_P0DXB0", "UniRef:UniRef90_D1J7M6" ], "nt": "GTGCAATTAGCATGCGATAATGTTAATAATATTATTGCTAAAGCTATTATTGGCAAGGAAGTTTCTAAACAAAAAGAATTAGATAATTTAATGATTAATCTTGATGGAACTGAAACAAAATCAAAATTAGGGGCAAATGCAATATTAGCTGTTTCATTAGCAATTGCAAAAGCTGGTGCCATTGAAGCAAATCTTCCATTATATAAATATTTAGCAAAATTAAACAACCAAGTAGCATACAAAATGCCAGTGCCAATGCTAAACGTTATTAATGGTGGAGAACATGCTTCAAATACTATTGACTTCCAAGAATTTATGATAATGCCTATTGGGGCAAAAACATTTAAAGAATCATTGCAAATGGCAAACTTTGTATTTCATACATTAGCAAAATTATTGAAACAAGCAGGTCATGGAACACAAGTTGGAGATGAAGGTGGATTTGCACCAAACCTACACACTCATGAAGAAGCATTGGACTTTTTAGTAAATGCAATTAAAGTCGCAGGATATAATCCAGCCACTTCAGGAGAAAAAGCAGTAGCAATTTGTTTGGATGCTGCAAGTTCAGAACTTTATCATTCAGATACAAAGTTATATGTATTTAAAAAATTCAAGAAAGCAATTGAAGAAAAACGCCCAGGATTTGAAAAATATCAAAATGTTAAATATTCATTTACTAGCGATGAATTTGTTGAATATTATGGCCATTTAATTGAAAAATACCCAATAATTTCTATTGAAGATTCACATGATGAAAACGATTGA", "aa": "MQLACDNVNNIIAKAIIGKEVSKQKELDNLMINLDGTETKSKLGANAILAVSLAIAKAGAIEANLPLYKYLAKLNNQVAYKMPVPMLNVINGGEHASNTIDFQEFMIMPIGAKTFKESLQMANFVFHTLAKLLKQAGHGTQVGDEGGFAPNLHTHEEALDFLVNAIKVAGYNPATSGEKAVAICLDAASSELYHSDTKLYVFKKFKKAIEEKRPGFEKYQNVKYSFTSDEFVEYYGHLIEKYPIISIEDSHDEND", "aa_hexdigest": "dea217ee008d5861a358579ce0e7cd65", "start_type": "GTG", "rbs_motif": "GGxGG", "genes": [ "eno" ], "truncated": "both", "pseudogene": { "start": 144231, "stop": 145499, "observations": { "mutation": [ 144536, 145319 ], "stop": [ 144536, 145319 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7M6", "query_cov": 1.0, "subject_cov": 0.5579868708971554, "identity": 0.992, "score": 494.0, "evalue": 2.95e-173, "gene_start": 1, "gene_end": 255, "reference_start": 67, "reference_end": 321, "reference_sequence": "MSKIIKINAYEVLDSRGNPTVKVELNTELAYSEALVPSGASTGSKEALELRDKGTKYENNWFGGKGVQLACDNVNNIIAKAIIGKEVSKQEELDNLMINLDGTETKSKLGANAILAVSLAIAKAGAIEANLPLYKYLAKLNNQVAYKMPVPMLNVINGGEHASNTIDFQEFMIMPIGAKTFKESLQMANFVFHTLAKLLKQAGHGTQVGDEGGFAPNLHTHEEALDFLVNAIKVAGYNPATSGEKAVAICLDAASSELYHSDTKLYVFKKFKKAIEEKRPGFEKYQNVKYSFTSDEFVEYYGHLIEKYPIISIEDSHDENDWDGFIKMNKRFGNKVQLVGDDLIVTNPKYIQMAIDKHAINASLIKINQIGSLTETFKAITMSQKAGFVAIISHRSGETEDTFIADLAVALNTGEIKTGSLSRTDRIAKYNRLLKIESELGNEAKFEGKNSFYNLKN" }, "paralog": false, "identity": 0.9928909952606635, "score": 818.0, "evalue": 1.22e-303, "description": "Internal stop codon at 144536, 145319. Nonsense mutation around 144536, 145319.", "psc": { "uniref90_id": "UniRef90_D1J7M6", "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_P0DXB0", "cog_id": "COG0148", "cog_category": "G", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7M6", "EC:4.2.1.11", "UniRef:UniRef50_P0DXB0", "COG:COG0148", "COG:G", "KEGG:K01689", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P0DXB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0DXB0" ], "product": "Enolase" } }, "id": "DFOKDJOJIG_301", "locus": "GCA013367855_00266" }, { "type": "cds", "sequence": "contig_1", "start": 145317, "stop": 145499, "strand": "-", "frame": 2, "gene": null, "product": "Phosphopyruvate hydratase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V5C5X0" ], "nt": "ATGTCAAAAATTATTAAGATTAATGCCTATGAAGTTTTAGATAGTAGAGGAAATCCAACAGTTAAAGTTGAATTAAATACTGAACTTGCATATTCTGAAGCATTAGTACCATCAGGAGCTTCAACAGGATCAAAAGAAGCACTTGAATTACGCGACAAAGGAACAAAATACGAAAATAATTGA", "aa": "MSKIIKINAYEVLDSRGNPTVKVELNTELAYSEALVPSGASTGSKEALELRDKGTKYENN", "aa_hexdigest": "6327a23b922b52ad6b82638088f018a6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A920A3H1", "query_cov": 0.9166666666666666, "subject_cov": 0.873015873015873, "identity": 0.527, "score": 55.1, "evalue": 8.27e-09, "valid": false, "product": "Enolase N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A7V5C5X0" }, "pscc": { "uniref50_id": "UniRef50_A0A7V5C5X0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V5C5X0" ], "product": "Phosphopyruvate hydratase" }, "genes": [], "id": "DFOKDJOJIG_302", "locus": "GCA013367855_00267" }, { "type": "cds", "sequence": "contig_1", "start": 145703, "stop": 146284, "strand": "+", "frame": 2, "gene": null, "product": "DUF3196 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LJ90", "UniRef:UniRef90_D1J7M7" ], "nt": "ATGAATAACATTGATAATAATTTATTTTTTATTGAAGAAGAAATTAAAAAAATTGAAAAAATTGCAAAAGAAGACCCACTTAAAGCAATTTATGTTTTAGATGAAGACAAAAAACAATTCTCTCTAAAAGAAGACTTGGATGATTTAGATGCATTGAGGGATAACATTGAGTTTCAACTTAAGAAACAAAATTTAATAACCTCAACAAAACTTGATACATTAAGTTTAATAAATAGTTTAAAAAACAAAAAAATGGGTTATGCATTTATGCTGAGCTACAACGAACTTAAAAAACGAGATGATATAGCAAAATATGCAATTGAATTTCAAGATTTTTTTAAAGGAAATGATTTTGATAGTAAAGGATTTCAAACATTGATTTATGACTTATTAGTCAATAGTAATATTGATTTTGAATACGATATTCAAAATAAAAAAATTAATCCCAAAAAATTAGGATCATTATTAAAGAATGCTGAAATTCAAAAGATGCAAGATGAAATTATTGCAACTTTTGACAAAGATATTTCAAAAAACAAGGTTGCAAGGCAAGTGTTTTCAGCGTATTTATTTCAAAATTGA", "aa": "MNNIDNNLFFIEEEIKKIEKIAKEDPLKAIYVLDEDKKQFSLKEDLDDLDALRDNIEFQLKKQNLITSTKLDTLSLINSLKNKKMGYAFMLSYNELKKRDDIAKYAIEFQDFFKGNDFDSKGFQTLIYDLLVNSNIDFEYDIQNKKINPKKLGSLLKNAEIQKMQDEIIATFDKDISKNKVARQVFSAYLFQN", "aa_hexdigest": "8bd514b37dc1362b077023c5f16b6a4c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M7", "query_cov": 1.0, "subject_cov": 0.8143459915611815, "identity": 0.995, "score": 365.0, "evalue": 5.17e-127, "valid": true, "uniref50_id": "UniRef50_A0A3G8LJ90" }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LJ90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LJ90" ], "product": "DUF3196 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_303", "locus": "GCA013367855_00268" }, { "type": "cds", "sequence": "contig_1", "start": 146297, "stop": 146416, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACGAAACAAAAGACGATTATTTAAAAATATCACACGTAATTGAAGTAATTTTAGGTAATCAAACGTCAGATTCACTTTCCAAAGAAGAAAAAGAGTTATATAACATTTTTAAATAA", "aa": "MHETKDDYLKISHVIEVILGNQTSDSLSKEEKELYNIFK", "aa_hexdigest": "4a515e3582b79afebef79e2aa6754ce3", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4596.1293, "isoelectric_point": 5.079553031921385 }, "id": "DFOKDJOJIG_304", "locus": "GCA013367855_00269" }, { "type": "cds", "sequence": "contig_1", "start": 146657, "stop": 147889, "strand": "+", "frame": 2, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "COG:COG0544", "COG:O", "EC:5.2.1.8", "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083", "SO:0001217", "UniRef:UniRef50_A0A501XAI0", "UniRef:UniRef90_D1J7M8" ], "nt": "TTGGATAGTATTTTAGATAATATTTATAAAGAACAAATAATTAGCCAAATAACCAAAGATGATGAAATTGTTGGACATGCAAATTTAAGAATTAAAGATATAAATGAAGAAAAAGCAGAAATTTCATTTGTATTTCCACTTTTCCCAGAAGTTAAATTGGGAGATTATAAAAAATTGGGTGTTAAATTAGGATCATGTGAATTAACTAAAAAAGATTTAAATGATGCAAAATCACAAATAGCTTCAAACTATGTAGTTATGCTAGATTCTAAAGAACCAATTAAAGAAAAAGATCAAGTAAACTTTAATTTCAAAGGCTTTATTGACGGAAAACCATTTGACGGCGGAGAAGCTGAAAACTTTGATTTAGTAATTGGATCTAAACAATTTATTCCAGGCTTTGAAGACAAAATGATTGGCCTAAAAGCAGGAGAAGAAAAAGATTTAGATTTAGTATTTCCAGAAGATTATCATGCTAAGAATTTAGCAGGAAAATCTGTTGTTTTCCATGTAAAAATAAATTTTGTTAAAACTCCAAATTATCCAACAATCGATGATAATTTTGTAAAAGAAATTAATTTGCCAAATGTTAAAAATGTTGCTGAATTTGAAGAATATGTAAAATTTATTGCTTTAAAGAACAAATTAGCAAATGTTAAAAATGAATTTATTAATGAAGCAGTTAAAAAATTATCAGAACAATCAGAAGTTTCTTTATCAGAATCATTAGTTGAAGAAGAAGCAGATAAATACTATCAAAATTTCCTTAACAATTTAAAACAACAACAAATTACTGAAAAAGATTATTACGAATTTTCAAAGAGTACAAAAGAAGAATCATTAAATGTATTTAAAAAACAAGCCCAAATAAACTTAAAACAAATGTTTGTATTAGGTGCTATTGCCAAAGCTGAATCTTTAAAAGTAACATTAGAAGATTATGAAAAAGAAGTTGAAAAATTGGCAAAAATATACGGCTTAGCTCCTGAACATGTAAAACAAATTTTGAAATTTGAAAATGTTGAAAATAATTTAATTAATGAAAGAATTAGTGAAATTTTACTAAAAGAAAATGACACTAAAGCATTTGAAAACTTTGACAAACTATTAAAAGAAGTCGAAGAATATGAAGCTAAACAAACTCAAGCTATTGTAGACGAAGCTAAACGCAAAAGAGAAGAAGCAGAAAAAATAGCTAAAGAATCAAAAGAAAACATTGACGAACAAAAATAA", "aa": "MDSILDNIYKEQIISQITKDDEIVGHANLRIKDINEEKAEISFVFPLFPEVKLGDYKKLGVKLGSCELTKKDLNDAKSQIASNYVVMLDSKEPIKEKDQVNFNFKGFIDGKPFDGGEAENFDLVIGSKQFIPGFEDKMIGLKAGEEKDLDLVFPEDYHAKNLAGKSVVFHVKINFVKTPNYPTIDDNFVKEINLPNVKNVAEFEEYVKFIALKNKLANVKNEFINEAVKKLSEQSEVSLSESLVEEEADKYYQNFLNNLKQQQITEKDYYEFSKSTKEESLNVFKKQAQINLKQMFVLGAIAKAESLKVTLEDYEKEVEKLAKIYGLAPEHVKQILKFENVENNLINERISEILLKENDTKAFENFDKLLKEVEEYEAKQTQAIVDEAKRKREEAEKIAKESKENIDEQK", "aa_hexdigest": "642a1df443ea706b2a09d98ea8836fbd", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M8", "query_cov": 1.0, "subject_cov": 0.8613445378151261, "identity": 0.995, "score": 760.0, "evalue": 1.43e-275, "valid": true, "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_A0A501XAI0", "cog_id": "COG0544", "cog_category": "O", "go_ids": [ "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501XAI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501XAI0" ], "product": "Trigger factor" }, "genes": [ "tig" ], "id": "DFOKDJOJIG_305", "locus": "GCA013367855_00270" }, { "type": "cds", "sequence": "contig_1", "start": 147981, "stop": 149207, "strand": "+", "frame": 3, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038", "KEGG:K03070", "SO:0001217", "UniRef:UniRef50_Q98RA6", "UniRef:UniRef90_D1J7M9" ], "nt": "ATGAAAATATTTGAATTTAAATCAACTGAAATGAGAATTGCAGAGGCAACCCTTAAGAGGATAAATGCCCTAGAACCAGAAATATCAAAATTAAGCAACGAGGAATTGCAACAAAAAACTATTGAGTTTAAGTCGCGTTTAAAAGCAGGAGAAACGCCAGAACAAATTAGACCTGAAACGTTTGCTGTTTGCAGAGAAGCAACAAAACGTATTTTAGGCAAAAGACCATTTGATGTTCAAATGATAGGCGGAATAATTTTAGACCTAGGTTCTGTAGCGGAAATGAAAACCGGTGAAGGTAAAACTATTACTTCTATAGCACCAATTTATTTAAACGCAATTACTGGTCAATCTGTTATTGTTTCAACAGTTAATGAATATCTAGCAGAACGTGATGCTCAAGAAATGGGGCAAGTTTTTAACTTTTTAGGTTTGAGTGTTGGAATAAACAAATCTCAAATGTCAAGTGAGTTAAAAAGAGAAGCATATGCTTGCGATATTGTTTATTCTGTTCATTCTGAATTAGGTTTTGATTATTTACGTGACAATATGGCAATGAGTAAAGAAGAAAAAGTTCAACGCGGGCTTGATTTTATTTTACTAGATGAAGTAGATTCAATATTGATTGATGAAGCAAAAACTCCTTTAATTATTTCAGGTGGCGATAATGACGAAAGCAATTTGTATACAATTGCAGATTTGTTTGTAAGAACATTAAGTCAAGATGATTATTTTATTGATGAAGAAACAAAATCTGTTTACCTAACAGATAAAGGAATTGAAAAGGCTAATAAATATTTCAATTTTAAAAATCTTTATGATATAGAAAATTCTGAACTTGTTCATAGAATTCAAAATGCTTTAAGAGCACATAAAGTAATGAAATTAGATGTTGAATATATTGTTAGAAACGACAAAATTGAATTAGTTGATTCCTTCACAGGTAGAATAATGGAAGGAAGAGCTTATTCAGAAGGATTGCAACAAGCAATTCAAGCAAAAGAACGCGTTGAAATTGAATCAGAAACCAAAACATTAGCAACAATTACTTATCAAAATTTTTTCCGTCTATTTAAAAAAATAAGTGGTATGACAGGAACAGCAAAAACAGAAGAAAAAGAATTTATTGATATTTATAATATGAGGGTCAATGTTGTTCCAACAAATAAACCAATAGCTAGATTTGATGATAAAGATGAAATCTATGTTGATATGCACAGTAAATGA", "aa": "MKIFEFKSTEMRIAEATLKRINALEPEISKLSNEELQQKTIEFKSRLKAGETPEQIRPETFAVCREATKRILGKRPFDVQMIGGIILDLGSVAEMKTGEGKTITSIAPIYLNAITGQSVIVSTVNEYLAERDAQEMGQVFNFLGLSVGINKSQMSSELKREAYACDIVYSVHSELGFDYLRDNMAMSKEEKVQRGLDFILLDEVDSILIDEAKTPLIISGGDNDESNLYTIADLFVRTLSQDDYFIDEETKSVYLTDKGIEKANKYFNFKNLYDIENSELVHRIQNALRAHKVMKLDVEYIVRNDKIELVDSFTGRIMEGRAYSEGLQQAIQAKERVEIESETKTLATITYQNFFRLFKKISGMTGTAKTEEKEFIDIYNMRVNVVPTNKPIARFDDKDEIYVDMHSK", "aa_hexdigest": "ae83a3a804cfa4d25aff8f17034ae6fd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034361D20", "query_cov": 0.9534313725490197, "subject_cov": 0.9328537170263789, "identity": 0.584, "score": 445.0, "evalue": 3.84e-152, "valid": false, "product": "DEAD/DEAH box helicase", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_A0A939UGC5", "kegg_orthology_id": "K03070" }, "genes": [ "secA" ], "truncated": "3-prime", "pseudogene": { "start": 147981, "stop": 149510, "observations": { "mutation": [ 149205 ], "stop": [ 149205 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7M9", "query_cov": 1.0, "subject_cov": 0.4722222222222222, "identity": 1.0, "score": 780.0, "evalue": 2.28e-277, "gene_start": 1, "gene_end": 408, "reference_start": 1, "reference_end": 408, "reference_sequence": "MKIFEFKSTEMRIAEATLKRINALEPEISKLSNEELQQKTIEFKSRLKAGETPEQIRPETFAVCREATKRILGKRPFDVQMIGGIILDLGSVAEMKTGEGKTITSIAPIYLNAITGQSVIVSTVNEYLAERDAQEMGQVFNFLGLSVGINKSQMSSELKREAYACDIVYSVHSELGFDYLRDNMAMSKEEKVQRGLDFILLDEVDSILIDEAKTPLIISGGDNDESNLYTIADLFVRTLSQDDYFIDEETKSVYLTDKGIEKANKYFNFKNLYDIENSELVHRIQNALRAHKVMKLDVEYIVRNDKIELVDSFTGRIMEGRAYSEGLQQAIQAKERVEIESETKTLATITYQNFFRLFKKISGMTGTAKTEEKEFIDIYNMRVNVVPTNKPIARFDDKDEIYVDMHSKWKAVTEEVKRVYKKKQPILIGTAQVEDSEILHEYLLSEGIPHTVLNAKQDASEAEIISKAGQVGAVTIATNMAGRGTDIKPSKEALELGGLYVLGTEKAESRRIDNQLKGRSGRQGDVGYTKFFLSLDDQLILRFSVQDHWKEIFKEYGEDPIPGEAIRKAFLRAQKKIEGFNFDNRKSVLNFDDVIRQQRDLIYEQRDLILNRDDLGTIIRKMISVAAEKTVNNPFFIKNNSLDLIEFVKHLNNNFMILTNKEFSVEELVEYDREELIQYLIETWNKCYDQLRQNIIDKYGISSLINSERNIILSVFDAAWQDHINIMDRLRRSTNLVQYSQKNPYQVYTQLGSKKFKELTDRIALECTINLMNNYDAIPSTNTDVDLSWLTNAFEEKTDMAQTTSKNQQALEFINYLLKSERDHLIKEGLGEQEVDDKLKEVERSILKEFQFQLASLQTNEEKK" }, "paralog": false, "identity": 0.9960707269155207, "score": 977.0, "evalue": 0.0, "description": "Internal stop codon at 149205. Nonsense mutation around 149205.", "psc": { "uniref90_id": "UniRef90_D1J7M9", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_Q98RA6", "cog_id": "COG0653", "cog_category": "U", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7M9", "EC:7.4.2.8", "UniRef:UniRef50_Q98RA6", "COG:COG0653", "COG:U", "KEGG:K03070", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RA6" ], "product": "Protein translocase subunit SecA" } }, "id": "DFOKDJOJIG_306", "locus": "GCA013367855_00271" }, { "type": "cds", "sequence": "contig_1", "start": 149307, "stop": 149627, "strand": "+", "frame": 3, "gene": "secA", "product": "SecA family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920T749" ], "nt": "TTGCTATCTGAAGGCATACCTCATACAGTTTTAAATGCAAAGCAAGATGCTTCAGAAGCAGAAATTATTTCTAAAGCGGGACAAGTAGGAGCAGTTACAATTGCCACAAACATGGCTGGACGTGGAACAGATATCAAGCCTTCAAAAGAAGCACTAGAATTAGGCGGATTGTATGTTTTAGGAACTGAAAAAGCTGAATCAAGAAGAATTGATAATCAACTAAAAGGACGTTCTGGCCGTCAAGGAGATGTAGGATATACAAAATTCTTTTTATCATTAGATGACCAATTAATTTTGCGTTTTTCAGTTCAAGATCACTGA", "aa": "MLSEGIPHTVLNAKQDASEAEIISKAGQVGAVTIATNMAGRGTDIKPSKEALELGGLYVLGTEKAESRRIDNQLKGRSGRQGDVGYTKFFLSLDDQLILRFSVQDH", "aa_hexdigest": "4cd803eb0ed4b784c964843ec9d98f33", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920T749", "query_cov": 0.8113207547169812, "subject_cov": 0.9247311827956989, "identity": 0.698, "score": 119.0, "evalue": 2.96e-33, "valid": false, "product": "SecA family profile domain-containing protein", "uniref50_id": "UniRef50_A0A920T749" }, "pscc": { "uniref50_id": "UniRef50_A0A920T749", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920T749" ], "product": "SecA family profile domain-containing protein" }, "genes": [ "secA" ], "id": "DFOKDJOJIG_307", "locus": "GCA013367855_00272" }, { "type": "cds", "sequence": "contig_1", "start": 149691, "stop": 150032, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGGCTCAAAAAAAGATTGAAGGGTTTAACTTTGATAATAGAAAAAGCGTCCTAAATTTCGATGACGTAATTAGACAACAACGTGATTTAATTTATGAACAACGTGATCTAATTTTAAATAGAGATGATCTTGGAACAATAATTAGAAAAATGATATCAGTTGCAGCAGAAAAAACAGTAAATAACCCATTTTTTATAAAAAACAATAGTTTAGATTTAACAGAATTTGTAAAGCATCTAAATAACAATTTTATGATCTTAACAAACAAAGAATTTAGTGTTGAAGAATTAGTTGAATATGATCGCGAAGAATTAATTCAATACTTAATTGAAACTTGA", "aa": "MRAQKKIEGFNFDNRKSVLNFDDVIRQQRDLIYEQRDLILNRDDLGTIIRKMISVAAEKTVNNPFFIKNNSLDLTEFVKHLNNNFMILTNKEFSVEELVEYDREELIQYLIET", "aa_hexdigest": "1f72255b6d70bd3f48b2009624615bb7", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13531.256399999984, "isoelectric_point": 5.040334129333496 }, "id": "DFOKDJOJIG_308", "locus": "GCA013367855_00273" }, { "type": "cds", "sequence": "contig_1", "start": 150159, "stop": 150575, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAGATTAAGAAGAAGTACAAATTTAGTTCAATATTCTCAAAAGAACCCATATCAAGTTTACACTCAATTGGGTTCAAAGAAATTCAAAGAATTAACAGACAGAATTGCTTTGGAATGCACAATTAATTTAATGAATAATTACGACGCAATACCTTCAACAAATACTGATGTTGATTTATCATGGTTAACAAATGCTTTTGAAGAAAAAACAGATATGGCTCAAACAACTTCAAAGAACCAGCAAGCTCTTGAATTTATCAATTATTTATTGAAGTCAGAACGTGATCATTTGATTAAAGAAGGTTTAAACGAACAAGAAGTGGATGATAAACTAAAAGAAGTGGAAAGATCAATATTAAAAGAGTTCCAATTCCAACTTGCTTCTTTGCAAACTAATGAAGAAAAAAAATAA", "aa": "MDRLRRSTNLVQYSQKNPYQVYTQLGSKKFKELTDRIALECTINLMNNYDAIPSTNTDVDLSWLTNAFEEKTDMAQTTSKNQQALEFINYLLKSERDHLIKEGLNEQEVDDKLKEVERSILKEFQFQLASLQTNEEKK", "aa_hexdigest": "3123fd67a22115d94af31cfdf036537a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16230.035699999991, "isoelectric_point": 5.129798698425293 }, "id": "DFOKDJOJIG_309", "locus": "GCA013367855_00274" }, { "type": "cds", "sequence": "contig_1", "start": 150584, "stop": 150832, "strand": "-", "frame": 3, "gene": "yjhB", "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)", "db_xrefs": [ "COG:COG1051", "COG:F", "EC:3.6.1.17", "KEGG:K01518", "SO:0001217", "UniRef:UniRef50_D1J7N0", "UniRef:UniRef90_D1J7N0" ], "nt": "TTGCTCAAGGGTTTTAGAGAAGTCAATAAATATATTATTGGAAAATTAATATCTAAAGAAGTTGTTTATTTTATTGCTAAGCCAACATCTTTTGATTTAATTAAGCAAGATAGTGAAATTAAGGTAGTTGAATGGCAAGATACAACAAATGCATTGTTATATCGCATAACATACGAAAGTGATTGTGAAATATTGAAAAAGGCTGTTGAATTTTATTGCGAATATATAACAAAAAATAACGGGTTTTAA", "aa": "MLKGFREVNKYIIGKLISKEVVYFIAKPTSFDLIKQDSEIKVVEWQDTTNALLYRITYESDCEILKKAVEFYCEYITKNNGF", "aa_hexdigest": "b54876451f72abc9d2566b155740c11a", "start_type": "TTG", "rbs_motif": null, "genes": [ "yjhB" ], "truncated": "3-prime", "pseudogene": { "start": 150584, "stop": 151015, "observations": { "mutation": [ 150925 ], "stop": [ 150925 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N0", "query_cov": 1.0, "subject_cov": 0.5734265734265734, "identity": 0.976, "score": 165.0, "evalue": 3.84e-51, "gene_start": 1, "gene_end": 82, "reference_start": 62, "reference_end": 143, "reference_sequence": "MKKEKSCGAIIFKEENDNLFVLLVEQTAGHWGFPKGHVEKNETEEETAIREVKEETNIDIKLLKGFREVNKYIIGKLISKEVVYFIAKPTSFDLIKQDSEIKVVEWQDTTNALLYRITYESDCEILKKAVEFYCEYITKNNEF" }, "paralog": false, "identity": 0.9790209790209791, "score": 281.0, "evalue": 3.9e-99, "description": "Internal stop codon at 150925. Nonsense mutation around 150925.", "psc": { "uniref90_id": "UniRef90_D1J7N0", "gene": "YjhB", "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)", "ec_ids": [ "3.6.1.17" ], "uniref50_id": "UniRef50_D1J7N0", "cog_id": "COG1051", "cog_category": "F", "kegg_orthology_id": "K01518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N0", "EC:3.6.1.17", "UniRef:UniRef50_D1J7N0", "COG:COG1051", "COG:F", "KEGG:K01518" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N0" ], "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)" } }, "id": "DFOKDJOJIG_310", "locus": "GCA013367855_00275" }, { "type": "cds", "sequence": "contig_1", "start": 150923, "stop": 151015, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGAGAAGTCGTGTGGCGCTATTATTTTCAAAGAAGAAAATGATAATCTTTTTGTTTTATTAGTTAAACAAACAGCAGGCCATTGA", "aa": "MKKEKSCGAIIFKEENDNLFVLLVKQTAGH", "aa_hexdigest": "f69ec7416ae76837c62eceaccdc3fb9b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3391.956, "isoelectric_point": 7.923631858825685 }, "id": "DFOKDJOJIG_311", "locus": "GCA013367855_00276" }, { "type": "cds", "sequence": "contig_1", "start": 151213, "stop": 151605, "strand": "-", "frame": 1, "gene": null, "product": "DUF4199 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U5", "UniRef:UniRef90_A0A454C9U5" ], "nt": "ATGGATATTAACAATATTATAAAAAATAATATAAAAAGATTATTAATAACAACCGCATTTGGCTTTTTAGCGATTTTGATTTCTTTTTTGATTACTGGGATATTTTCAATACAATCACTTTCAAATAATAGCCGTCTTGCAATATCCTGCTTTGGACAAATAATTTGCGGTTTTTATGTTGTTTATTATATTTTGAATTTCTTTTTAGTATATAGATTTAAATCAGAAATTGCAAACAAAAAGATATTAAAAACTCTATTAATAATCAGCATTTTTATTTTGATATGTGGAATTTGTTTTATTGTTAATGGTTTGATTTTACCTTCAATTAGAAAAACATACATTCAAGCAATAATGACTAAAAATAATAATTTATTACAAAGTATACTTTGA", "aa": "MDINNIIKNNIKRLLITTAFGFLAILISFLITGIFSIQSLSNNSRLAISCFGQIICGFYVVYYILNFFLVYRFKSEIANKKILKTLLIISIFILICGICFIVNGLILPSIRKTYIQAIMTKNNNLLQSIL", "aa_hexdigest": "604c14b09473ca14a45b00ef0290a453", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 151102, "stop": 151605, "observations": { "mutation": [ 151185, 151215 ], "stop": [ 151185, 151215 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9U5", "query_cov": 1.0, "subject_cov": 0.7784431137724551, "identity": 0.985, "score": 238.0, "evalue": 9.72e-79, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MDINNIIKNNIKRLLITTAFGFLAILISFLITGIFSIQSLSSNSRLAISCFGQIICGFYVVYYILNFFLVYRFKSEIANKKILKTLLIISIFILICGICFIVNGLILPSIRKTYIQAVMTKNNNLLQSILWQGKVIKVEMWISSTIFLVAIASSLILCSFIFKKMNN" }, "paralog": false, "identity": 0.9760479041916168, "score": 310.0, "evalue": 1e-109, "description": "Internal stop codon at 151185, 151215. Nonsense mutation around 151185, 151215.", "psc": { "uniref90_id": "UniRef90_A0A454C9U5", "product": "DUF4199 domain-containing protein", "uniref50_id": "UniRef50_A0A454C9U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9U5", "UniRef:UniRef50_A0A454C9U5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U5" ], "product": "DUF4199 domain-containing protein" } }, "id": "DFOKDJOJIG_312", "locus": "GCA013367855_00277" }, { "type": "cds", "sequence": "contig_1", "start": 151689, "stop": 152573, "strand": "+", "frame": 3, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "COG:COG2812", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360", "KEGG:K02343", "SO:0001217", "UniRef:UniRef50_D1J7N2", "UniRef:UniRef90_D1J7N2" ], "nt": "GTGGCACTTACTAATAAATATTTAGCATTGTATAGAAAATATAGACCAAAAATTTTTGATGATATAAAAGGCCAAGATCACATTGTAACAACATTGAAAAATATTATTTTGAATAATAAATTGAGTCATGCGTATTTATTTTGTGGCCCCCATGGAAATGGAAAAACTTCAACTGCTAAAGTTTTTGCAAACACAATTAATTGTTTACATAATCAAAGCGATATATTAAAACCATGTGAAGAATGTATCAAAAATGTCGATAGAAATCTAGATATTATAGAAATAGATGCTGCTTCTAATACCGGCATTGATGACATTAGAGAATTAAAAGAAAAAATAAAACATTTACCAACACAAAGCAAATATAAAATTTATATTATTGATGAAGTTCATATGCTTTCAAAAGCAGCCTTTAACGCTTTATTAAAAACAATTGAAGATCCACCTAGTCACATAATTTTTATTCTAGCAACAACTGATCCCCAAAAAATACCAGAAACTATTTTAAGTAGGGTTCAAAGATTCAATTTTAAACTTATGACAATTGAAACATTAGTTTCTCAATTGAAGGATATTTTCAATAAAGAAAATATTGAATATGAAGATGATGCAATTAATGTTATAGCTAAATTAGGAAATGGTAGCCTTAGAGATTCATTAAGTATTGCAGACCATGTTGCAATATATTGTGCCAATAAAGTTATTAAGAAAGAAGTTGTTGAACAATTATTTGGAATTGTAAATATAGATAATAAAATAGAGTTATTAAATTGTATGTCAATGCATGATATCAATAAAACATTGAATCTTGCAAGAGAATTATTTAGTCAAGGTATTGATATAAACATCTTGATAAACGATTTAATAAACATTTTAAAAGAATGA", "aa": "MALTNKYLALYRKYRPKIFDDIKGQDHIVTTLKNIILNNKLSHAYLFCGPHGNGKTSTAKVFANTINCLHNQSDILKPCEECIKNVDRNLDIIEIDAASNTGIDDIRELKEKIKHLPTQSKYKIYIIDEVHMLSKAAFNALLKTIEDPPSHIIFILATTDPQKIPETILSRVQRFNFKLMTIETLVSQLKDIFNKENIEYEDDAINVIAKLGNGSLRDSLSIADHVAIYCANKVIKKEVVEQLFGIVNIDNKIELLNCMSMHDINKTLNLARELFSQGIDINILINDLINILKE", "aa_hexdigest": "599f28bc4d3f563ffeffba8cd9517212", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00244E6DAE", "query_cov": 0.8163265306122449, "subject_cov": 0.967741935483871, "identity": 0.629, "score": 304.0, "evalue": 8.38e-101, "valid": false, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A7V0IXX6", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "genes": [ "dnaX" ], "truncated": "3-prime", "pseudogene": { "start": 151689, "stop": 152876, "observations": { "mutation": [ 152571 ], "stop": [ 152571 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N2", "query_cov": 1.0, "subject_cov": 0.4407796101949025, "identity": 1.0, "score": 579.0, "evalue": 4.82e-203, "gene_start": 1, "gene_end": 294, "reference_start": 1, "reference_end": 294, "reference_sequence": "MALTNKYLALYRKYRPKIFDDIKGQDHIVTTLKNIILNNKLSHAYLFCGPHGNGKTSTAKVFANTINCLHNQSDILKPCEECIKNVDRNLDIIEIDAASNTGIDDIRELKEKIKHLPTQSKYKIYIIDEVHMLSKAAFNALLKTIEDPPSHIIFILATTDPQKIPETILSRVQRFNFKLMTIETLVSQLKDIFNKENIEYEDDAINVIAKLGNGSLRDSLSIADHVAIYCANKVIKKEVVEQLFGIVNIDNKIELLNCMSMHDINKTLNLARELFSQGIDINILINDLINILKEWIIFNKTRDQSLVSTDFKILLQIKIDDVKAHYYIETLIDQLHSIAISDSPEDYFEILLLKLCNYLSPKEEYKNSAVMPKISNEEFNNFNSQEKQVINKNNIYKSLDLSSIANGFNANNSTIQNSSDNNSTEDLANNSKKIDDLLEKTAEIYSLDQTSEFEVADISNQIISNNQISIDSNVDNQETADLQISENWKDLVNEDLISKENSENKHVPLETIIDCLLIKQYLKNNMLNDGRSNFTDIDAIKYGMLSQKITDKNEINILSDFKIIFSTNDFILLTCDIDEKVYDLNINSHNENIMAALNKVFDRYLYAYAITKGEILEAKKYWKANINEIRAKKVKPLEDLNLKYNKTVQKDVAWAQEIFGDKFKLNK" }, "paralog": false, "identity": 0.9873417721518988, "score": 773.0, "evalue": 1.16e-281, "description": "Internal stop codon at 152571. Nonsense mutation around 152571.", "psc": { "uniref90_id": "UniRef90_D1J7N2", "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_D1J7N2", "cog_id": "COG2812", "cog_category": "L", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N2", "EC:2.7.7.7", "UniRef:UniRef50_D1J7N2", "COG:COG2812", "COG:L", "KEGG:K02343", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N2" ], "product": "DNA polymerase III subunit gamma/tau" } }, "id": "DFOKDJOJIG_313", "locus": "GCA013367855_00278" }, { "type": "cds", "sequence": "contig_1", "start": 152631, "stop": 153152, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAATTAAAATTGATGATGCTAAAGCACACTATTACATTGAAACATTGATAGATCAATTGCATAGTATTGCCGTAAGTGATAGTCCAGAAGATTATTTCGAAATTCTGTTGCTAAAGTTATGTAATTACTTAAGTCCAAAAGAAGAATACAAAAATTCTGCAGTAATGCCAAAAATTAGCAATGAAGAATTTAATAATTTTAATAGTCAAGAAAAGCAGGCCATTAATAAAAATAATATTTACAAATCATTGGATTTATCCTCAATAGCAAATGGATTCAATGCAAACAATTCCACAATACAAAATTCAAGTGATAATAATTCAACAGAAGACTTAGCTAATAATTCAAAAAAGATTGATGACTTATTAGAAAAAACTGCAGAAATATATTCATTAGATCAAACATCAGAGTTTGAAGTTGCTGATATTTCTAATCAAATAATAAGTAATAATCAAATTAGTATAGATTCAAATGTTGATAACCAAGAAACAGCCGATTTACAAATATCGGAAAATTGA", "aa": "MQIKIDDAKAHYYIETLIDQLHSIAVSDSPEDYFEILLLKLCNYLSPKEEYKNSAVMPKISNEEFNNFNSQEKQAINKNNIYKSLDLSSIANGFNANNSTIQNSSDNNSTEDLANNSKKIDDLLEKTAEIYSLDQTSEFEVADISNQIISNNQISIDSNVDNQETADLQISEN", "aa_hexdigest": "6a381ee48208e52bdc6d0c1403e92165", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19540.055799999987, "isoelectric_point": 4.198037147521972 }, "id": "DFOKDJOJIG_314", "locus": "GCA013367855_00279" }, { "type": "cds", "sequence": "contig_1", "start": 153213, "stop": 153650, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAACAATAATCGACTGTTTATTAATAAAACAATATTTGAAAAATAATATGTTAAATGATGGTCGTAGTAATTTTACAGACATTGATGCTATTAAATATGGTATGTTAAGTCAAAAAATAACAGACAAAAATGAAATTAACATCTTATCAGACTTTAAAATTATTTTTTCAACGAATGATTTTATTTTGTTAACATGTGATATAGATGAAAAAGTCTACGATTTAAATATAAATTCACATAATGAAAATATTATGGCTGCATTGAATAAAGTTTTTGATAGATATCTTTATGCTTATGCCATTACAAAAGGCGAGATATTAGAAGCAAAGAAATATTGGAAAGAAAATATAAACGAAATAAGAGCCAAAAAAGTTAAACCTTTGGAAGATTTAAATTTAAAATATAATAAAACAGTTCAAAAAGATGTTGCTTGA", "aa": "METIIDCLLIKQYLKNNMLNDGRSNFTDIDAIKYGMLSQKITDKNEINILSDFKIIFSTNDFILLTCDIDEKVYDLNINSHNENIMAALNKVFDRYLYAYAITKGEILEAKKYWKENINEIRAKKVKPLEDLNLKYNKTVQKDVA", "aa_hexdigest": "2949c40311fade33050a2fc703aa4d12", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16967.400699999995, "isoelectric_point": 5.8799028396606445 }, "id": "DFOKDJOJIG_315", "locus": "GCA013367855_00280" }, { "type": "cds", "sequence": "contig_1", "start": 153716, "stop": 154015, "strand": "+", "frame": 2, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "KEGG:K09747", "RefSeq:WP_012855373.1", "SO:0001217", "UniParc:UPI0001BC20AC", "UniRef:UniRef100_D1J7N3", "UniRef:UniRef50_Q98RF9", "UniRef:UniRef90_D1J7N3" ], "nt": "ATGAGTATGAACATGAATGAAATGCTTAAAAAAGCACGTAGAATTCAAACTGAAATGGAAAAAGATGAACAAGAAATTCAAAAGAAGGAATTTACTATTGAAAAGCAAGGCATAACCGTTGTTATGAATGGTTTAAGAAAATTAGTGTCTATAAAATTTAATCCAGCATTAATCGATCCCGATGACCCTGAATTATTAGAAGATTTATTGATGCTTGCGGTTAATGAAGCAATAGAAACAGTTGATAAGGCTTTTGATGAAATTAATGCTAAATATTCTGATAGTGGCCTTTCATTCTAA", "aa": "MSMNMNEMLKKARRIQTEMEKDEQEIQKKEFTIEKQGITVVMNGLRKLVSIKFNPALIDPDDPELLEDLLMLAVNEAIETVDKAFDEINAKYSDSGLSF", "aa_hexdigest": "77242d963041dcb031811ba880313b74", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20AC", "ncbi_nrp_id": "WP_012855373.1", "uniref100_id": "UniRef100_D1J7N3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20AC", "RefSeq:WP_012855373.1", "UniRef:UniRef100_D1J7N3" ] }, "ips": { "uniref100_id": "UniRef100_D1J7N3", "uniref90_id": "UniRef90_D1J7N3" }, "psc": { "uniref90_id": "UniRef90_D1J7N3", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_Q98RF9", "cog_id": "COG0718", "cog_category": "K", "kegg_orthology_id": "K09747", "go_ids": [ "GO:0003677" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RF9" ], "product": "Nucleoid-associated protein MYPU_0500" }, "genes": [ "ybaB" ], "id": "DFOKDJOJIG_316", "locus": "GCA013367855_00281" }, { "type": "cds", "sequence": "contig_1", "start": 154015, "stop": 154605, "strand": "+", "frame": 1, "gene": "recR", "product": "Recombination protein RecR", "db_xrefs": [ "COG:COG0353", "COG:L", "RefSeq:WP_044329430.1", "SO:0001217", "UniParc:UPI0005C83B92", "UniRef:UniRef100_A0A6A8PYU1", "UniRef:UniRef50_D1J7N4", "UniRef:UniRef90_D1J7N4" ], "nt": "ATGAGCAATATTGAAATACAAAAACTAGAAGAACTTTTAAAAACGGTTTTTGATTTTACTAAAAAACAATCTCAAAAATTTATATTCAACATTTTTAAATCATCAATTGAAGATATAGACCAAGTTTGCAATCAAATAAAAGCATTGAGAAATTCAATAAAAAAGTGCAACATTTGTAATAGATATAACGAAAATACAATTTGCAATATTTGCTTGGATACTAATCGAACAAAACAACTTATGTTAGTTGGTAATTCGTTAGATATTGATAAATTTGAAAATATTGGTTTGTATAAAGGAAAATATTTTGTATTTGATCAAAATATAAATTCAAAGAATGAAGATTCTATAGTTTCAAGTTTATTAGAAAAATTAATAGATATATTAAAAGATAAGAATGAACTAATTCTTTCACTTTCCCCAACTATTGAGGGTCAATTTAATATGCATTATATTAAAAAGTTCTTGAAAAACAAAATAAATTTTACAAATATTTTTCAATTATCAACAGGTATCCCTTTAGGTTCATCAGTTGAATATATTGATCAAGATACATTAAAAGAATCATTATTGAATAAAATTAAAATATAG", "aa": "MSNIEIQKLEELLKTVFDFTKKQSQKFIFNIFKSSIEDIDQVCNQIKALRNSIKKCNICNRYNENTICNICLDTNRTKQLMLVGNSLDIDKFENIGLYKGKYFVFDQNINSKNEDSIVSSLLEKLIDILKDKNELILSLSPTIEGQFNMHYIKKFLKNKINFTNIFQLSTGIPLGSSVEYIDQDTLKESLLNKIKI", "aa_hexdigest": "5515fc29b6a7fb195d5f0b46016062ed", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0005C83B92", "ncbi_nrp_id": "WP_044329430.1", "uniref100_id": "UniRef100_A0A6A8PYU1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C83B92", "RefSeq:WP_044329430.1", "UniRef:UniRef100_A0A6A8PYU1" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PYU1", "uniref90_id": "UniRef90_D1J7N4" }, "psc": { "uniref90_id": "UniRef90_D1J7N4", "gene": "RecR", "product": "Recombination protein RecR", "uniref50_id": "UniRef50_D1J7N4", "cog_id": "COG0353", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_D1J7N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N4" ], "product": "Recombination protein RecR" }, "genes": [ "recR" ], "id": "DFOKDJOJIG_317", "locus": "GCA013367855_00282" }, { "type": "cds", "sequence": "contig_1", "start": 154617, "stop": 154922, "strand": "+", "frame": 3, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0006233", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_A0A448ZVP3", "UniRef:UniRef90_D1J7N5" ], "nt": "ATGTCAAAAGGAAAATTTGTAGTATTTGAAGGGATGGATGGTTCAGGAAAAACAACTATTATAAAGAGATTAAAAGAATATTTAGAGTCTAAAAATAGACTAGATGATTTCGTATTTACAAGAGAACCAGGAAGCTCACATTCAAAAGAAGCAGAAGAAATAAGACAGCTAATTTTAGATAATGATAATTCTTTTAGTGTTATGGTGGATGCTTTGTTATTTGCAGCAAGTAGAAGGTTAAATCTTGAAAAATGCGTTTGGCCAGGATTGGAAAAGAATAAAACGGTTATTTCAGATAGGTATTGA", "aa": "MSKGKFVVFEGMDGSGKTTIIKRLKEYLESKNRLDDFVFTREPGSSHSKEAEEIRQLILDNDNSFSVMVDALLFAASRRLNLEKCVWPGLEKNKTVISDRY", "aa_hexdigest": "ca6abdc408c1b8a60c8fb286be18969c", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "tmk" ], "truncated": "3-prime", "pseudogene": { "start": 154617, "stop": 155225, "observations": { "mutation": [ 154920, 155145 ], "stop": [ 154920, 155145 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N5", "query_cov": 1.0, "subject_cov": 0.47417840375586856, "identity": 0.98, "score": 200.0, "evalue": 1.1e-63, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MSKGKFVVFEGMDGSGKTTIIKKLKEYLESKNRLDDFIFTREPGSSHSKEAEEIRQLILDNDNSFSVMVDALLFAASRRLNLEKCVWPGLEKNKTVISDRYWTSSFVYQGVLGGLGLKKVKDINNIATEETTPDLTIFFDMEPELSVERITQIRKTKTDRLESTNVEYYKKLRDSYWYVINQGNEKYEIIKSDCNIDELFKRVLNVLKENKII" }, "paralog": false, "identity": 0.9801980198019802, "score": 393.0, "evalue": 4.89e-142, "description": "Internal stop codon at 154920, 155145. Nonsense mutation around 154920, 155145.", "psc": { "uniref90_id": "UniRef90_D1J7N5", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_A0A448ZVP3", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0006233" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N5", "EC:2.7.4.9", "UniRef:UniRef50_A0A448ZVP3", "COG:COG0125", "COG:F", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0006233" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVP3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVP3" ], "product": "Thymidylate kinase" } }, "id": "DFOKDJOJIG_318", "locus": "GCA013367855_00283" }, { "type": "cds", "sequence": "contig_1", "start": 155037, "stop": 155147, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCCAGAACTATCAGTTGAAAGAATTACGCAAATTCGAAAAACAAAAACTGATAGATTAGAATCTACCAATGTTGAATATTACAAAAAACTAAGAGATTCTTATTGA", "aa": "MEPELSVERITQIRKTKTDRLESTNVEYYKKLRDSY", "aa_hexdigest": "a43bed897f7f5d7fb1812dc0843d9f5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4420.9522000000015, "isoelectric_point": 8.064688682556149 }, "id": "DFOKDJOJIG_319", "locus": "GCA013367855_00284" }, { "type": "cds", "sequence": "contig_1", "start": 155296, "stop": 155451, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAACAAAAATTTGAGTAAAGTATTGGAAAATTCAATAAAAAACAATAAACTTCAACAAGTTTATTTACTATTTTCACGTGCAAATCAAGATATTGATTTAGATATTTTAGAATTTGTTAGATTAGTTAATGATGAAAAACAAATAAGCTGA", "aa": "MINKNLSKVLENSIKNNKLQQVYLLFSRANQDIDLDILEFVRLVNDEKQIS", "aa_hexdigest": "95054a296a5df88e7f26091e738f7f78", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6005.851499999999, "isoelectric_point": 6.081283378601074 }, "id": "DFOKDJOJIG_320", "locus": "GCA013367855_00285" }, { "type": "cds", "sequence": "contig_1", "start": 155674, "stop": 156129, "strand": "+", "frame": 1, "gene": "dnaX", "product": "DNA polymerase III subunit", "db_xrefs": [ "COG:COG2812", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J7N6", "UniRef:UniRef90_D1J7N6" ], "nt": "TTGCATACAATAAAGCCCCAAAACGTTTTGAAAACTATCAAATCAAGATGTTTTATTATTGATATTCGTGATGATTTTAATGCTGTAGCTAGCGTTGAAAATTTTTTTATTACATATAAAATTGACGTAAGCAAGCAAAAACAATTTAGGGAACTTGCTGATTTAATATACAATTCACCAGCAAATCTATTTAATCTTATTTTGTTTTATATTCAACAATTAAATATAGAAAATTATAAAAATATTATTCATTTTTCTATTTTTATTTTTAAACAAATTTACGAATTAAAAAATAGCATAAAAAAAGATAAAAATATTAGTAAATTTGATTCTATTTCAATATTAAATATTATTAATAATTTATATAATTTTGTTGAATTAATTGATAACAATAATTGTATTTTCAATATTCAAAAAGCAAGGCTAATTTTAAAATTAAAGGAAGAATATGAATAA", "aa": "MHTIKPQNVLKTIKSRCFIIDIRDDFNAVASVENFFITYKIDVSKQKQFRELADLIYNSPANLFNLILFYIQQLNIENYKNIIHFSIFIFKQIYELKNSIKKDKNISKFDSISILNIINNLYNFVELIDNNNCIFNIQKARLILKLKEEYE", "aa_hexdigest": "c1b263deeb65a13b279c481e7e6aa125", "start_type": "TTG", "rbs_motif": null, "genes": [ "dnaX" ], "truncated": "5-prime", "pseudogene": { "start": 155374, "stop": 156129, "observations": { "mutation": [ 155449 ], "stop": [ 155449 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N6", "query_cov": 1.0, "subject_cov": 0.5451263537906137, "identity": 0.98, "score": 291.0, "evalue": 6.32e-98, "gene_start": 1, "gene_end": 151, "reference_start": 127, "reference_end": 277, "reference_sequence": "MINENLSKVLENSIKNNKLQQVYLLFSRSNQDIDLDILEFVRLVNDEKQISWQQCLQKEYCFIIDGGQVEISKENLQEFLNRVSNSSLFANKYKFLIIKCVENITLKAANSILKFLENPPFNTIILLHTIKPQNVLKTIKSRCFIIDIRDDFNAVASVENFFITYKIDVSKQKQFRELADLIYNSPANLFNLILFYVQQLNIENYKNIIHFSIFIFKQIYELKNSIKKDKNISKFDSISILNIINNLYNFVELIDNNNCIFNIQKAKLILKLKEEYE" }, "paralog": false, "identity": 0.9681274900398407, "score": 478.0, "evalue": 5.41e-174, "description": "Internal stop codon at 155449. Nonsense mutation around 155449.", "psc": { "uniref90_id": "UniRef90_D1J7N6", "gene": "DnaX", "product": "DNA polymerase III subunit", "uniref50_id": "UniRef50_D1J7N6", "cog_id": "COG2812", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N6", "UniRef:UniRef50_D1J7N6", "COG:COG2812", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N6" ], "product": "DNA polymerase III subunit" } }, "id": "DFOKDJOJIG_321", "locus": "GCA013367855_00286" }, { "type": "cds", "sequence": "contig_1", "start": 156122, "stop": 156835, "strand": "+", "frame": 2, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0008649", "GO:0008757", "KEGG:K07056", "RefSeq:WP_036432737.1", "SO:0001217", "UniParc:UPI000512E542", "UniRef:UniRef100_UPI000512E542", "UniRef:UniRef50_D1J7N7", "UniRef:UniRef90_D1J7N7" ], "nt": "ATGAATAATAAAATTTATATAGTTGGAACTCCAATTGGAAATTTAGAAGATATAACATATAGAGCAATTAAGGTTTTACAATCCAGCGATATAATTTTGTGTGAAGATATAAGAGTTAGTCAAAAATTATTGCAACATTATGAAATTAATAACAAGCGCCTAATTGCATACCATAAATTTAATGAAAAAGAAATGACACCTAAAATTATTGAATTATTGCAACAAGGGATGATTATTTCATTGATTTCTGATGCCGGTATGCCTTGCATTTCTGATCCTGGTTTTGAAGTTATTAAAGCAGCCAAGGCAGCAAATATTGGCATTGATGTAATTGGCGGCCCAACCGCTCTAATACATGCAATAATAAAGGCTAATTTAGACAATTGCTTCACATTTCTAGGCTTTTTAAAAGATAAGTCAGGTGCAAGACAAAATCAATTAAAATCCTTGACATTTGGCACATATGTTGCTTACGTTTCACCTCATAAATTGCTTTCAACTATTAAAGATTTTGAGATTATTTTTCAAGAGCAAGTTAAATTATTTTTAATTAAAGAAATGACAAAAATGTTTGAAACCTCATTTGAAGGAACACCTCAAGAAGTTCTAACACAACTAAGCGAAGAAGATCAACGCGGTGAATATACTCTTGTATTTAGTTTCAAAGAACCAAAGAAACTTAAAATTAACAAATATAAAAAAGAAATAAAATAA", "aa": "MNNKIYIVGTPIGNLEDITYRAIKVLQSSDIILCEDIRVSQKLLQHYEINNKRLIAYHKFNEKEMTPKIIELLQQGMIISLISDAGMPCISDPGFEVIKAAKAANIGIDVIGGPTALIHAIIKANLDNCFTFLGFLKDKSGARQNQLKSLTFGTYVAYVSPHKLLSTIKDFEIIFQEQVKLFLIKEMTKMFETSFEGTPQEVLTQLSEEDQRGEYTLVFSFKEPKKLKINKYKKEIK", "aa_hexdigest": "d3808ec098ebdbf8e5c9dd3975aecb69", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000512E542", "ncbi_nrp_id": "WP_036432737.1", "uniref100_id": "UniRef100_UPI000512E542", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512E542", "RefSeq:WP_036432737.1", "UniRef:UniRef100_UPI000512E542" ] }, "ips": { "uniref100_id": "UniRef100_UPI000512E542", "uniref90_id": "UniRef90_D1J7N7" }, "psc": { "uniref90_id": "UniRef90_D1J7N7", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_D1J7N7", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0008649", "GO:0008757" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N7" ], "product": "Ribosomal RNA small subunit methyltransferase I" }, "genes": [ "rsmI" ], "id": "DFOKDJOJIG_322", "locus": "GCA013367855_00287" }, { "type": "cds", "sequence": "contig_1", "start": 156879, "stop": 157142, "strand": "-", "frame": 2, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02968", "RefSeq:WP_012855378.1", "SO:0001217", "UniParc:UPI0001BC20B1", "UniRef:UniRef100_D1J7N8", "UniRef:UniRef50_Q98PR3", "UniRef:UniRef90_D1J7N8" ], "nt": "ATGGCAAACATTAAATCAAAACAAAAAGCAATATTATCTAACGAAAAAGCAAACATCCGTAATTCAGCCGTTAAATCATCAGTTAAAACAGCTATTAAGAAAGCAAAAATTGCTGCTGTTAATAAAGATCCTAAAGCTGCTGAATTTCAAGCTAATGCTCACCATGAAATTGACAAAGCTGTTTCAAAGGGCGTTTTACATCAAAACAATGGTTCTAGAAAAGCTAGCCGTTTAGATGCATTTATTGCTAAAAATAACAACTAA", "aa": "MANIKSKQKAILSNEKANIRNSAVKSSVKTAIKKAKIAAVNKDPKAAEFQANAHHEIDKAVSKGVLHQNNGSRKASRLDAFIAKNNN", "aa_hexdigest": "19721fc9eea4c2bbc137fa79a30a1504", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC20B1", "ncbi_nrp_id": "WP_012855378.1", "uniref100_id": "UniRef100_D1J7N8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20B1", "RefSeq:WP_012855378.1", "UniRef:UniRef100_D1J7N8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7N8", "uniref90_id": "UniRef90_D1J7N8" }, "psc": { "uniref90_id": "UniRef90_D1J7N8", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_Q98PR3", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PR3" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "DFOKDJOJIG_323", "locus": "GCA013367855_00288" }, { "type": "cds", "sequence": "contig_1", "start": 157236, "stop": 157511, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAACAAAGAAAAAATATGATGGATATCTTAAATATTCAAAAAATAATGAATCTGAGTTGATAAATTATTATCTAACTAATAATAAAAATATCTATTTAAAGTTAAACAATGAACAAGAAATCGTAGCTTATGTGCCACATAAGTATATGAATACAATAATATTGGATAATTTTTTAAAATCATCAGTTCCTAAACTTCTAGAAATAAAAACAAAAATTGAGCAAAAGAAAATACTTGCAATTGATATAGAAAAAAATAAATTTTATTTATGA", "aa": "MVTKKKYDGYLKYSKNNESELINYYLTNNKNIYLKLNNEQEIVAYVPHKYMNTIILDNFLKSSVPKLLEIKTKIEQKKILAIDIEKNKFYL", "aa_hexdigest": "fa6d4e73a62b64492009d9400fdc9578", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10910.65289999999, "isoelectric_point": 9.398410606384278 }, "id": "DFOKDJOJIG_324", "locus": "GCA013367855_00289" }, { "type": "cds", "sequence": "contig_1", "start": 157545, "stop": 157868, "strand": "+", "frame": 3, "gene": "ygjP", "product": "YgjP-like metallopeptidase domain-containing protein", "db_xrefs": [ "COG:COG1451", "COG:R", "SO:0001217", "UniRef:UniRef50_D1J7N9", "UniRef:UniRef90_D1J7N9" ], "nt": "ATGTTAAATTTATTTGAATTAAATAAGAATTTAAAAATCTTAAAACAAGGTATAGAATTTACTCTAAATAAATATTTAAAAAAAATATTACTGGAACTATCAACTTTATATGTAGAAAAATTTTGTAAATTATTATGCATACCATCCGAGTCAATAAAAGTAAGAATTTTGAAGAAGAATAATGCCTGGGGAACAAATCATTTTTTAAAAAGAACAATAACTTTTTCAGATCGGTTAATTTTTTACAGAAAAGAAGTTTTGGAATATGTTGCTTGTCATGAAGTTGTTCATTTTATTTATCAAAACCATTCATCTGAATTTTGA", "aa": "MLNLFELNKNLKILKQGIEFTLNKYLKKILLELSTLYVEKFCKLLCIPSESIKVRILKKNNAWGTNHFLKRTITFSDRLIFYRKEVLEYVACHEVVHFIYQNHSSEF", "aa_hexdigest": "8d0718263d78085603a24ef2e33ca10b", "start_type": "ATG", "rbs_motif": null, "genes": [ "ygjP" ], "truncated": "both", "pseudogene": { "start": 157245, "stop": 157940, "observations": { "mutation": [ 157509, 157866 ], "stop": [ 157509, 157866 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N9", "query_cov": 1.0, "subject_cov": 0.45726495726495725, "identity": 0.981, "score": 205.0, "evalue": 2.89e-65, "gene_start": 1, "gene_end": 107, "reference_start": 104, "reference_end": 210, "reference_sequence": "MVTKKKYDGYLKYSKNNESELINYYLTNNKNIYLKLNKEQEIVAYVPRKYMNTIILDNFLKSSVPKLLEIKTKIEQKKILAIDIEKNKFYLWGKESKFLIQKNMLNLFELNKNLKILKQGIEFTLNKYLKKILLELSTSYVEKFCKLLCIPSESIKVRILKKNNAWGTNHFLKRTITFSDRLIFYRKEVLEYVACHEVVHFIYQNHSFEFWNMVKKYIPNFKQIKKELKDKIFI" }, "paralog": false, "identity": 0.974025974025974, "score": 446.0, "evalue": 1.27e-162, "description": "Internal stop codon at 157509, 157866. Nonsense mutation around 157509, 157866.", "psc": { "uniref90_id": "UniRef90_D1J7N9", "gene": "YgjP", "product": "YgjP-like metallopeptidase domain-containing protein", "uniref50_id": "UniRef50_D1J7N9", "cog_id": "COG1451", "cog_category": "R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N9", "UniRef:UniRef50_D1J7N9", "COG:COG1451", "COG:R" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N9" ], "product": "YgjP-like metallopeptidase domain-containing protein" } }, "id": "DFOKDJOJIG_325", "locus": "GCA013367855_00290" }, { "type": "cds", "sequence": "contig_1", "start": 157953, "stop": 158294, "strand": "+", "frame": 3, "gene": null, "product": "NERD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7P0", "UniRef:UniRef90_D1J7P0" ], "nt": "ATGGAAAATAAAACAAAATTTATTAATGGAATTATTATAGGATTAGTTTTGCTATTAGTAATAGTTATAATTCTAATTTCAGTTTCAATTGTTCTTTCTAAAAAGAATAGAAGATTTTTAAAAGATCAAAAAGGATTTTTAGATTTATACAATACACAATTAAAAAATATTAGTGAATCTATAAAAAATTGCAAATTTATAGGTGCCAACGATTATTCTTATAACGATCAAAGAATACACTTAGATGATATATTTGTTACTGATAAAATTATTATTGTATTAAAGCCTTATATGTATGACTCTGACTCTTCTATTCAAGGTGACTATTTTAGCAACGAATGA", "aa": "MENKTKFINGIIIGLVLLLVIVIILISVSIVLSKKNRRFLKDQKGFLDLYNTQLKNISESIKNCKFIGANDYSYNDQRIHLDDIFVTDKIIIVLKPYMYDSDSSIQGDYFSNE", "aa_hexdigest": "1868255c4cd4c8ef4ab644c163c0b4b3", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 157953, "stop": 158591, "observations": { "mutation": [ 158292 ], "stop": [ 158292 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P0", "query_cov": 1.0, "subject_cov": 0.5330188679245284, "identity": 0.973, "score": 194.0, "evalue": 4.72e-61, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MENKTKFINGIIIGLVLLLVIVIILISVSIVLSKKNRRFLKDQKGFLDSYNTQLKNISESIKNCKFIGANDYSYNDQRIHLDDIFVTDKIIFILKPYMYDSDSSIQGDYFSNEWILTKSNGKKSKIPNQLISLKKSMNNFGLLFSNEMSICSIFVHNLSTELNIEDLPSFQGIYNVKDLCASIKSVYNSNINSMYNQSVSTIFNVLNNKIAK" }, "paralog": false, "identity": 0.9575471698113207, "score": 355.0, "evalue": 6.08e-127, "description": "Internal stop codon at 158292. Nonsense mutation around 158292.", "psc": { "uniref90_id": "UniRef90_D1J7P0", "product": "NERD domain-containing protein", "uniref50_id": "UniRef50_D1J7P0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P0", "UniRef:UniRef50_D1J7P0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7P0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7P0" ], "product": "NERD domain-containing protein" } }, "id": "DFOKDJOJIG_326", "locus": "GCA013367855_00291" }, { "type": "cds", "sequence": "contig_1", "start": 158361, "stop": 158591, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTTGGACTATTGTTTTCTAATGAAATGAGTATATGTTCAATTTTTGTACATAATTTAAGCACGGAATTAAATATAGAAGATGTGCCATCGTTTCAAGGAATTTGCAATATTAAAGATTTATGTGCTTCAATAAAATCTGTATATAATTCGAATATTAATTCAATGTATAACCAATCTATTTCAACTATTTTCAATGTTTTAAATAATAAAATTGCAAAATAA", "aa": "MNNFGLLFSNEMSICSIFVHNLSTELNIEDVPSFQGICNIKDLCASIKSVYNSNINSMYNQSISTIFNVLNNKIAK", "aa_hexdigest": "3f4bad52d60dedad36c99758f0851bdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8510.662199999995, "isoelectric_point": 5.537618446350096 }, "id": "DFOKDJOJIG_327", "locus": "GCA013367855_00292" }, { "type": "cds", "sequence": "contig_1", "start": 158750, "stop": 158938, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAGAACAAAAAACAGTATTAGAAAAATACTATATTTCTGAAGCTTTTGATAAAGATAGAAATATTTCATTTAACTTTAAAAAGGCAAATAAGGGCATTTCAGGAAACTTTTTAAATTTTTCTAGCGCAGTTGAAGAATTTATTAAAGTTAGTGAAAATACACAAAACCCAACCAAATGTTGA", "aa": "MAKEQKTVLEKYYISEAFDKDRNISFNFKKANKGISGNFLNFSSAVEEFIKVSENTQNPTKC", "aa_hexdigest": "c6147096031e44b0c32b36152fe9cf71", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7128.958700000002, "isoelectric_point": 8.787637138366701 }, "id": "DFOKDJOJIG_328", "locus": "GCA013367855_00293" }, { "type": "cds", "sequence": "contig_1", "start": 159062, "stop": 159172, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3B5093", "UniRef:UniRef90_UPI001E3B5093" ], "nt": "TTGGTTGAAAAACCTGCAGCAAAAGCTAAGAAAGTTGAAGAACCAAAACAAGAATGTGAATGCTCATGCTCTTGTGATTGTTTAACCGGTATTGAAAACAACAAACACTGA", "aa": "MVEKPAAKAKKVEEPKQECECSCSCDCLTGIENNKH", "aa_hexdigest": "eb53802b4805d3520c8aa908a76e9c35", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 159062, "stop": 159334, "observations": { "mutation": [ 159170, 159182 ], "stop": [ 159170, 159182 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E3B5093", "query_cov": 1.0, "subject_cov": 0.4, "identity": 0.972, "score": 53.9, "evalue": 1.62e-08, "gene_start": 1, "gene_end": 36, "reference_start": 1, "reference_end": 36, "reference_sequence": "MVEKPASKAKKVEEPKQECECSCSCDCLTGIENNKHWKEYWTKKSSNNIVCSGIQDHKGGEKLFITLTVLLSLLTIINLILIILLATNVI" }, "paralog": false, "identity": 0.9111111111111111, "score": 140.0, "evalue": 3.26e-45, "description": "Internal stop codon at 159170, 159182. Nonsense mutation around 159170, 159182.", "psc": { "uniref90_id": "UniRef90_UPI001E3B5093", "uniref50_id": "UniRef50_UPI001E3B5093", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E3B5093", "UniRef:UniRef50_UPI001E3B5093" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E3B5093", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3B5093" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 3981.555799999999, "isoelectric_point": 5.667097663879395 }, "id": "DFOKDJOJIG_329", "locus": "GCA013367855_00294" }, { "type": "cds", "sequence": "contig_1", "start": 159688, "stop": 159975, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGCGTGAAGTTTTAGGTGAATTAAATATTAAATATAAAGAATTTATTGGTGAAGCAGCATTTTATGGACCTAAGGTTGATTTTCAAGTAAAAACTGCACTAAATAGAATTATTACTATGTCAACCTTACAATTAGACTTTTTATTACCATCAAGATTTGAAATGAAATTTGTTGATAGTAATGAACAATTTGTAACACCAGTATTAATTCATAGAGGTTTAATTGGAACTTATGAAAGATTTATTGCAACACTACTTGAACAAACAAAAGGTGTATTGCCATTTTGA", "aa": "MREVLGELNIKYKEFIGEAAFYGPKVDFQVKTALNRIITMSTLQLDFLLPSRFEMKFVDSNEQFVTPVLIHRGLIGTYERFIATLLEQTKGVLPF", "aa_hexdigest": "f194bf682ba591db6ef0c59e7468d337", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0029CA135A", "query_cov": 0.8736842105263158, "subject_cov": 0.8217821782178217, "identity": 0.578, "score": 102.0, "evalue": 1.71e-26, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.3" ], "uniref50_id": "UniRef50_Q71HR4", "kegg_orthology_id": "K01868" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 78, "aa_cov": 0.7894736842105263, "hmm_cov": 0.9743589743589743, "evalue": 1.1953336457503734e-14, "score": 50.41576385498047, "start": 14, "stop": 88 } ], "seq_stats": { "molecular_weight": 10973.738299999988, "isoelectric_point": 6.570439720153809 }, "id": "DFOKDJOJIG_330", "locus": "GCA013367855_00295" }, { "type": "cds", "sequence": "contig_1", "start": 159994, "stop": 160362, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGTTTGCCCCAATTAAAATGGATAACGAAACATTAGTTGCTAGACCTATGACTTGCCCACACCATATAATATTGTTCAATTCAACAAGACGTTCATACAAAGACTTGCCAATTAGATATTCAGAACAATCAAGGTTATATCGTTACGAAGCATCGGGTGCTTTAAGTGGACTTGAAAGAGTTAGAGGGATGGATTTAACAGAAGGTCACATTTTTGTTAGACCAGATCAAATAAAATCAGAATTTAAGCACTTGTATAAAATGATATTGCAAGCATTAAAAGATTTTAATATTGAAATAGATCATATTTCTTTATCATTAAGAGATCCACAAGATACTCAAAAATTCTTCCAAAATGATGAAATGTGA", "aa": "MFAPIKMDNETLVARPMTCPHHIILFNSTRRSYKDLPIRYSEQSRLYRYEASGALSGLERVRGMDLTEGHIFVRPDQIKSEFKHLYKMILQALKDFNIEIDHISLSLRDPQDTQKFFQNDEM", "aa_hexdigest": "97320dfde981601fae09eb5d9d7d2548", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 104, "aa_cov": 0.8360655737704918, "hmm_cov": 0.9807692307692307, "evalue": 7.884960671319453e-22, "score": 73.80873107910156, "start": 8, "stop": 109 } ], "seq_stats": { "molecular_weight": 14435.421899999981, "isoelectric_point": 6.96768741607666 }, "id": "DFOKDJOJIG_331", "locus": "GCA013367855_00296" }, { "type": "cds", "sequence": "contig_1", "start": 160381, "stop": 160500, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTCATAATGCAATTAGAGATTATGTTTTAAAACTTGATAAAAAATTTGGTTTTAGAGAAGTACTAACTCCACACTTTGGTGAAAAGAAGCTTTATGAAATAAGTGGACACTGA", "aa": "MKIHNAIRDYVLKLDKKFGFREVLTPHFGEKKLYEISGH", "aa_hexdigest": "a17315a2b28feeeb87eb514c8fb89092", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4659.415, "isoelectric_point": 9.5220609664917 }, "id": "DFOKDJOJIG_332", "locus": "GCA013367855_00297" }, { "type": "cds", "sequence": "contig_1", "start": 160513, "stop": 160674, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACAAGAATCTATGGTACTTGTTGGGAAACAAAAGATGAATTAGCAAAATATTTAGATGTCTTAAGGGAAAGAAAAGAAAGAGATCATCGTAAAATAGGAAGAGATTTAAATTTATTCACTTTTAACCAATTAGGTGGACAAGGATTCCCATTTTGA", "aa": "MLTRIYGTCWETKDELAKYLDVLRERKERDHRKIGRDLNLFTFNQLGGQGFPF", "aa_hexdigest": "56315c90616ed25ebc8a69f938de49a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6377.2321999999995, "isoelectric_point": 8.976658439636232 }, "id": "DFOKDJOJIG_333", "locus": "GCA013367855_00298" }, { "type": "cds", "sequence": "contig_1", "start": 160807, "stop": 161124, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCAAATAAAAAATTAAATCATTCAGCAAGCCATTTATTAGCGGCTGCTATATTAAAACTTTATCCAAATGCTAAATTGGCAATTGGACCAGCCATTGAAGAAGGGTTCTATTACGACTTTGAATTTGAAAATCCAATTTTAGAATCAGATCTTCCAAATATTGAAAAAGTAATGAAAGAATTAGCAAAAGAAGGTTACGAAATGCGAAAAGTTGGCATTGAACAATATTCTTTTGAAAATCAACCATATAAAAAAGAACTTTATGAAGAATTTAAAAAAGAAGGAAAAGAAATAACATTCTTTCAATATTGA", "aa": "MEANKKLNHSASHLLAAAILKLYPNAKLAIGPAIEEGFYYDFEFENPILESDLPNIEKVMKELAKEGYEMRKVGIEQYSFENQPYKKELYEEFKKEGKEITFFQY", "aa_hexdigest": "5b3a71cd39b162d39bb0cc2e71f1f051", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12319.913599999993, "isoelectric_point": 5.108199882507324 }, "id": "DFOKDJOJIG_334", "locus": "GCA013367855_00299" }, { "type": "cds", "sequence": "contig_1", "start": 161124, "stop": 161798, "strand": "-", "frame": 2, "gene": null, "product": "Tryptophan--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "nt": "ATGACGCAATTTAAAGATAAATCAGCTAAAATAAAAACTGCAAACGGTATGGAAACAATTCCAACAGGAATTTTAATGTATCCTACCTTAATGGTGGGCGATATATTACTATATAATGCAGATATTATTCCCGTTGGTATTGACCAAAAGCAACATGTTGAATTGACTAGAAATTTAGCAATTAGATTGAATAATAAATTCAATACAACGTACAAAATACCTGAACCATATATACCAGAAGTTGGGGCCAAAATAATGTCTTTAACTGACCCAAGTAAGAAAATGTCAAAATCTGATACAAATCCTAAATCAGCAATTTATTTATTAGATGATCCAGAAGATGCATACAAAAAAATATTAAAAGCTGTTACAGATTCAGAAGGAAAAATATATTTGTCAGATGAAAAGCCGGGCATAAAAAACCTTTTGACTATATATAGTGCTTTAAATGATATTTCATTACAAGAAAGCGCTGAAAAATTTAAAAACAGCAATTACGGTGAATTTAAAAATGCTGTCGCTCAATGTGTAAAGGAATTTTTAATCAAGATCCAAGATAAGTATAAAAATGCCTTGAATCAAATTGAAACATTGAGCAAAGAGGGAGCAAAAAAGGCAAGTGAAGTTGCCCAAATTAACTTAGATATGTTATTAAAAGGAATAGGATTAAGATAA", "aa": "MTQFKDKSAKIKTANGMETIPTGILMYPTLMVGDILLYNADIIPVGIDQKQHVELTRNLAIRLNNKFNTTYKIPEPYIPEVGAKIMSLTDPSKKMSKSDTNPKSAIYLLDDPEDAYKKILKAVTDSEGKIYLSDEKPGIKNLLTIYSALNDISLQESAEKFKNSNYGEFKNAVAQCVKEFLIKIQDKYKNALNQIETLSKEGAKKASEVAQINLDMLLKGIGLR", "aa_hexdigest": "fd7b8c8172c37627a113216ad05a7c0b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3B0PAD4", "query_cov": 1.0, "subject_cov": 0.9333333333333333, "identity": 0.71, "score": 323.0, "evalue": 1.59e-109, "valid": false, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_A0A3B0PAD4", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PAD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "product": "Tryptophan--tRNA ligase" }, "genes": [], "id": "DFOKDJOJIG_335", "locus": "GCA013367855_00300" }, { "type": "cds", "sequence": "contig_1", "start": 161838, "stop": 162125, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATTCTGAAAAGAAGACGTTATTAAGTGGAATTACTGCAACTGGTAAATTAACGCTGGCAAATTATATTGGTGCTATAAAAAATATGGTTAAATTGCAAGAAGAATATAACAATTACATTTTTATAGCAGATTTACATGCTCTAACGTTGCCAATTGATCCTAAGCAATTATCAAAAAATAAAAAGGATATTTTTGCACTTTATATTGCATGTGGAATTGACCCTAAAAAGAGTGTGTTATTTTTTCAATCAGATGTCATTCAACACGGATTAATGAATTGA", "aa": "MQNSEKKTLLSGITATGKLTLANYIGAIKNMVKLQEEYNNYIFIADLHALTLPIDPKQLSKNKKDIFALYIACGIDPKKSVLFFQSDVIQHGLMN", "aa_hexdigest": "a613fab4aa3aa15dabfc15552bb97150", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10644.364899999991, "isoelectric_point": 9.041578102111817 }, "id": "DFOKDJOJIG_336", "locus": "GCA013367855_00301" }, { "type": "cds", "sequence": "contig_1", "start": 162443, "stop": 162679, "strand": "+", "frame": 2, "gene": "ptsG2", "product": "PTS EIIB type-1 domain-containing protein", "db_xrefs": [ "COG:COG1264", "COG:G", "SO:0001217", "UniRef:UniRef50_N9UZL6", "UniRef:UniRef90_D1J7P4" ], "nt": "ATGCAAGATTTAATTTTATATTTGGGTGGAATTTCAAATATAGTTTCTTCATCTGCAACTCAAAAAATTGTAAGAATTTCATTTAAAAATAAAACAAATATTTCTATTGAAAAAATAAAAAAACTTAATGGCATATCTGGTATTGCATTTCAATCTTCGAATATATCATTAGTTGTTGGAAATTGTGCAAATTATTTATCTACATTACTTAATAAGGAACTAAACAATCATGAATAA", "aa": "MQDLILYLGGISNIVSSSATQKIVRISFKNKTNISIEKIKKLNGISGIAFQSSNISLVVGNCANYLSTLLNKELNNHE", "aa_hexdigest": "11e33cd5cb3c4b9cbb3e0592fb943538", "start_type": "ATG", "rbs_motif": null, "genes": [ "ptsG2" ], "truncated": "5-prime", "pseudogene": { "start": 162269, "stop": 162679, "observations": { "mutation": [ 162374 ], "stop": [ 162374 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P4", "query_cov": 1.0, "subject_cov": 0.5735294117647058, "identity": 1.0, "score": 148.0, "evalue": 1.31e-44, "gene_start": 1, "gene_end": 78, "reference_start": 59, "reference_end": 136, "reference_sequence": "ISYIGKTIIGEKMLNKNKFLYIFLIIITLGLVLIFWRKKYKILKTKNYLTTDNEITFSMQDLILYLGGISNIVSSSATQKIVRISFKNKTNISIEKIKKLNGISGIAFQSSNISLVVGNCANYLSTLLNKELNNHE" }, "paralog": false, "identity": 0.9852941176470589, "score": 258.0, "evalue": 2.46e-90, "description": "Internal stop codon at 162374. Nonsense mutation around 162374.", "psc": { "uniref90_id": "UniRef90_D1J7P4", "gene": "PtsG2", "product": "PTS EIIB type-1 domain-containing protein", "uniref50_id": "UniRef50_N9UZL6", "cog_id": "COG1264", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P4", "UniRef:UniRef50_N9UZL6", "COG:COG1264", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_N9UZL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UZL6" ], "product": "PTS EIIB type-1 domain-containing protein" } }, "id": "DFOKDJOJIG_337", "locus": "GCA013367855_00302" }, { "type": "cds", "sequence": "contig_1", "start": 162672, "stop": 163046, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114FF0", "UniRef:UniRef90_UPI0024114FF0" ], "nt": "ATGAATAACAAAGATTCTAAATATCAAACATTAATGGCTAATTTATTGAATATTGATACATATGATACTTCACTATATACATCTATAAATAGTGAAAATTTTTTTGATATTTACAACAATTTTGGTCAAGAAATATTTGATCAAATTTTTAAAAATTACAAATTTAATTGTATTTTAACCGAAGTCAAAAATAAAGAAATGCGCCAATTGCTAGAAAAGGCACAAAATAAGCAAGAAATAATAACGATTTATAAAGATAATAATGTCAGAATCGAACAATCAATATTTAAGATGCTATCAACTGATTTTGAAAAAGCTAAAACCTTATTAATTACTAAATTAGAATCAGAATTTCAAAAATCAATAGTAAATTGA", "aa": "MNNKDSKYQTLMANLLNIDTYDTSLYTSINSENFFDIYNNFGQEIFDQIFKNYKFNCILTEVKNKEMRQLLEKAQNKQEIITIYKDNNVRIEQSIFKMLSTDFEKAKTLLITKLESEFQKSIVN", "aa_hexdigest": "11dc00f925aeb645eb72db61d3602459", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0024114FF0", "query_cov": 1.0, "subject_cov": 0.8157894736842105, "identity": 1.0, "score": 153.0, "evalue": 1.46e-45, "valid": true, "uniref50_id": "UniRef50_UPI0024114FF0" }, "pscc": { "uniref50_id": "UniRef50_UPI0024114FF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114FF0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14779.667499999985, "isoelectric_point": 5.516190147399901 }, "id": "DFOKDJOJIG_338", "locus": "GCA013367855_00303" }, { "type": "cds", "sequence": "contig_1", "start": 163101, "stop": 163349, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATGTAGGTTTTTTATTTATTTCAATAAAAACGGATAAAAAGACAATCTTTGCTCCCTTATTATTTAAAGAAGTAACATTAGAAGTTAAAAATTCTTTGGTTTATTTGAAATCGAATTCAGATGTAAGAATAAATAGTAAGTTAGTAACATTTTTATCTCAAGAAGGTTTTTTGTTAAATATTGATAATTTAGATTTTTCTAATTTGTCAATCGAATCTATATTTGAGCATTTAAAAAAAATTTGA", "aa": "MHVGFLFISIKTDKKTIFAPLLFKEVTLEVKNSLVYLKSNSDVRINSKLVTFLSQEGFLLNIDNLDFSNLSIESIFEHLKKI", "aa_hexdigest": "3f3eadc5954fe1b6a13a61b8c2de92a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9433.956899999994, "isoelectric_point": 8.027168083190919 }, "id": "DFOKDJOJIG_339", "locus": "GCA013367855_00304" }, { "type": "cds", "sequence": "contig_1", "start": 163365, "stop": 163487, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCAATCACTAAGAAGCAATATTCCGCTTGTATCTCAAAGTGATATTCAAAACGTATCTATCGAATTTCATCCCGGAATGATTTTTGGATTTTATAACGTTTCTTCGGGATATTTATGA", "aa": "MPQSLRSNIPLVSQSDIQNVSIEFHPGMIFGFYNVSSGYL", "aa_hexdigest": "3b3542bb815f6037943a18df730e216e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4474.0337, "isoelectric_point": 5.3026460647583 }, "id": "DFOKDJOJIG_340", "locus": "GCA013367855_00305" }, { "type": "cds", "sequence": "contig_1", "start": 163494, "stop": 163682, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTATTGAAAACGATGAATTTGAAAGTATTTTAGTACCCGAAATTAATAAAAATGTTTATAGAGAAAAAATCAACACAAGTATTTTTAATAATAAATTTAAGTTATTTAAGGTACAAAAAACAAACTTTTCACAAGATGTTGCTACAGTGTCTGCACTATGTCAAGATACAATAATTTGA", "aa": "MKKIIENDEFESILVPEINKNVYREKINTSIFNNKFKLFKVQKTNFSQDVATVSALCQDTII", "aa_hexdigest": "82aa51f4507ea3369e6fa1a6adb94bd3", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7239.282900000003, "isoelectric_point": 7.844142341613771 }, "id": "DFOKDJOJIG_341", "locus": "GCA013367855_00306" }, { "type": "cds", "sequence": "contig_1", "start": 163764, "stop": 164582, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTCAAAAGAAGGCCGCCCTTGATGTATTGAGAAATAGACTAGGACAATTGAGCTTATTTTGTTTATTTGCTTTAAATGATAAAAATTTAAGACAAGAGGTATTTTACGAACCATTAAAAAAATTTATAGAAAAAATTGAGAATTTTTCTATTTCTCAAGTTGAAAAAGGAATTCAAATTTTTTCTGATGAAGATAAGGGCTATGTCGATACATTGAATGAAATAGAAGCACTGCCAAACTTAAATAACTTACTTCATTTCTATGCCTCAACATTGAATGCCAATTTAACAATAAATGATTTTAATACCCTAAGATTATTGGCAAAAGATATAAAATATGATGTTGAAGGACATACTTTTAATGATAAGAAAGAACTTAAAAAATATCTATACGAAGTAAATTTGAAAAGAAGACCATCAATATTTTCAATTTATCCAAGGTCAATAAAAGAAGCTAGTGAATTAATTATTAATGCGCCTGAATTATTTAAAATAAATGTAGATGAAGCAGTAAAATACATTGATAAAGTAGAGTTTGCCGAATTGGAAACTTTTGTTGAAAAATATAAAAATTTACTGATTAAAAAAACAATAAATGTAAGTAACTCAGCCGTTTTATCTAATATGTTATTTGCTAAATTAATAAATACAATTTCGAATTTCAATGATGAACAAAAACGTGAATATACATCGTTTGCAATGTCTATAAGAACAGGTAGCTTAAAACCATATATGTTTTTCCATAGATATAAAAACATAATTAAAGTATTATTTCCAATAATTGTAACTACTCCTGAATTAGATTTGTCAATGTGA", "aa": "MSQKKAALDVLRNRLGQLSLFCLFALNDKNLRQEVFYEPLKKFIEKIENFSISQVEKGIQIFSDEDKGYVDTLNEIEALPNLNNLLHFYASTLNANLTINDFNTLRLLAKDIKYDVEGHTFNDKKELKKYLYEVNLKRRPSIFSIYPRSIKEASELIINAPELFKINVDEAVKYIDKVEFAELETFVEKYKNLLIKKTINVSNSAVLSNMLFAKLINTISNFNDEQKREYTSFAMSIRTGSLKPYMFFHRYKNIIKVLFPIIVTTPELDLSM", "aa_hexdigest": "87c00da7256fcdfefac955d39ce4ccd3", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31726.381600000037, "isoelectric_point": 8.651479911804202 }, "id": "DFOKDJOJIG_342", "locus": "GCA013367855_00307" }, { "type": "cds", "sequence": "contig_1", "start": 164754, "stop": 164963, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGATATTGAATCTCTTTTAGATTACGCTACTGCTAGAGGAACTTATTCAATATTGCTAGATAAGAACTATCGTTCAAAGAGGGCATCATTAATGACATTCAGTTCTAAACATTTTTATGATTCCAAATTAGATGTAATAGATGATTTTAAATTAGCATTTTCTAGTGAAAGACCAATTGATGTAATTCAAGTTGATGGCGAATGA", "aa": "MGDIESLLDYATARGTYSILLDKNYRSKRASLMTFSSKHFYDSKLDVIDDFKLAFSSERPIDVIQVDGE", "aa_hexdigest": "88f9d4fb6c1a4b670a1b4e6bd3b74ede", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7884.7716, "isoelectric_point": 4.954848289489745 }, "id": "DFOKDJOJIG_343", "locus": "GCA013367855_00308" }, { "type": "cds", "sequence": "contig_1", "start": 165078, "stop": 165380, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "TTGACAAATGCAATATTTAGCGTTGAACCAGAACTAGAAAAAGCCTTATTTTCCGATAAAATAACAATAAAAAATATTGAAAACATTCAAGGTGACGAAGCTGATTTAGTCATTATGTCTGTTGTTTATGACAAAAAAACAGCATTGTTTGGAACATACGTTGCAAGGAAGGGTGGGAAAAACGCCCTAAATGTTGCCATATCAAGAGCAAGAGAAAAAATTATTGTTGTAAAATCAATTTATGCAGATGATGTTGAAATTAATGAACGTTCAACATCTGATATGCGTTTATTTAAAGAATGA", "aa": "MTNAIFSVEPELEKALFSDKITIKNIENIQGDEADLVIMSVVYDKKTALFGTYVARKGGKNALNVAISRAREKIIVVKSIYADDVEINERSTSDMRLFKE", "aa_hexdigest": "bc5c8960128a8bfaa6c0b0edf1e4fba1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 66, "aa_cov": 0.65, "hmm_cov": 0.9393939393939394, "evalue": 1.9638822868477047e-11, "score": 39.48163604736328, "start": 13, "stop": 77 } ], "seq_stats": { "molecular_weight": 11228.792499999994, "isoelectric_point": 5.540687751770019 }, "id": "DFOKDJOJIG_344", "locus": "GCA013367855_00309" }, { "type": "cds", "sequence": "contig_1", "start": 165474, "stop": 165761, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTACCAAAAGTAAGCAACCTTCAAATGGATGTATTGGCGAAATTAAACGAATTATTGAAGGAACTAAATGCTAACAATTTCGAAATTATTTCGAATTATTCAATAGGAACTAAATTATTAGACTTAGTCTTAATCAATAAAATTTCAAATAATATGATTATTGGTATCAATATTGACGATTTTAAATATTTGCCTGATTATAGAAAATATTTAAAATTTAAAGACGATTTTAATTTTTTACTATCAAAATCATATCCAATAATTAATATTTCTAGAATAAGTTGA", "aa": "MLPKVSNLQMDVLAKLNELLKELNANNFEIISNYSIGTKLLDLVLINKISNNMIIGINIDDFKYLPDYRKYLKFKDDFNFLLSKSYPIINISRIS", "aa_hexdigest": "1422797e7fc561dd24d12efa0e6a2073", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11058.878999999984, "isoelectric_point": 8.859583854675293 }, "id": "DFOKDJOJIG_345", "locus": "GCA013367855_00310" }, { "type": "cds", "sequence": "contig_1", "start": 165840, "stop": 166889, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9F07", "UniRef:UniRef90_UPI00215D9F07" ], "nt": "TTGTCTGAAGAAAATAACAATTTAAATGTAGAAGAAATTAAAGAAAACATTATAGAAAATAATGCCTTAAAAAACATAAAATCAAGCCAAATTAAACAAGGTCCTGAATTAAAAGAAATAGATTTTGAAAATGTTGATGACGAAACTTTTGAAGTTGATCAAGATTCTAATAATAAAATTTATGAATTAAATGAATCTGAGGTTGCTCAATTTGAAGAATTCCATAATAAGAATATAAATCATGAATTTAATGACCAAACCGAATCAAAGGCTGAATACTCAAAGGACTATGAAAAAGATTTTTTGATTAATTATAATGATGTTGAATTGGATAATACGCATATCGATCAGGAATTTCACATTGAAAATGAATTTGAATCAACAATAAATAATAGTAATGTCATAAAGATTGAAGATGTCACTTCATCATCGGCTAACGATGAATTAAAAGTAACTAATTCTAATGAACAAAAAATTTCTGCAAAAACAGCAAGTGAAGATATTGAATCGGGATTTTCAGTTGAAGATATAAACAAAGAAAATATTAGCAATTTATTTTCTAATACATTTGATGATCTTGATATAAATATAGCACCTGAAAGTGAACAACAAGTTGAAGCTAAACTTAATTATGATTCAGGCATTATTCAAGATAAGTTGCATGAGCAAAACGATGAATATTTTAATTTAGAAGAAAATAATATTGAAGAGCAACCAAAAAATAATAATGTTCAAAGCGCAGATGAGACATTAAAATCTCAATTAGTTTATATTGACAATAATAAAGAAGTAAACGATAAACAAAAAAGCTATGATTTTGCAGGTTTATTTGCTGACGAAAATGAAGAAACCTCTACAGATGTAAAAAACATGATAGACATGAATACTGCAGAATATAATCAATATATGAAAAATAAAGATATTGAACAAGATGAACAAAATAAAGAGATAACTAAAAATACTGCTGATGAAGAAATATTGAATTTTGATTTAATCGAAGATGAAAAAACTACGGAATTTAACGTGGAGGACAACAATGTCAAAAAATAG", "aa": "MSEENNNLNVEEIKENIIENNALKNIKSSQIKQGPELKEIDFENVDDETFEVDQDSNNKIYELNESEVAQFEEFHNKNINHEFNDQTESKAEYSKDYEKDFLINYNDVELDNTHIDQEFHIENEFESTINNSNVIKIEDVTSSSANDELKVTNSNEQKISAKTASEDIESGFSVEDINKENISNLFSNTFDDLDINIAPESEQQVEAKLNYDSGIIQDKLHEQNDEYFNLEENNIEEQPKNNNVQSADETLKSQLVYIDNNKEVNDKQKSYDFAGLFADENEETSTDVKNMIDMNTAEYNQYMKNKDIEQDEQNKEITKNTADEEILNFDLIEDEKTTEFNVEDNNVKK", "aa_hexdigest": "7ce9181796b84402a008a5a086b186a7", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 165540, "stop": 166889, "observations": { "mutation": [ 165759 ], "stop": [ 165759 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D9F07", "query_cov": 1.0, "subject_cov": 0.6856581532416502, "identity": 0.948, "score": 615.0, "evalue": 1.01e-218, "gene_start": 1, "gene_end": 349, "reference_start": 161, "reference_end": 509, "reference_sequence": "MRLFKEWLKFLDLSIIEQKNYLFLDNENKKYDEKTQTILLPKVSNLQMAVLAKLNELLKELNANNFEIISNYSIGTKLLDLVLINKISNNMIIGINIDDFKYLPDYRKYLKFKDDFNFLLSKSYPIINISRISWIVENKQILNKIKILIKHEEELNSQILLSEENNNLNVEEIKENIIENNAIKNIKSNQIKQSPELKEIDFENVDDETFEVDQDSNNKIYELNESEVAQFEEFHNKNINHEFNDQTESRAEYSKDYEKDFLINYNDIELDNTHIDQEFHIENEFESTINNSNAVKIEDATSSSANDELKITNSNEQKISAKTASEDIESGFSIEDINKENISNLFSNTFDDLDINMALESEQQVEAKLNYDSGIVQDKLHEQNDEYFNLEENDIEEQQKNNDVQSPDETLKSQLVYIDNNKEVNDKQKSYDFAGLFADENEETSTDVKNMIDMNTAEYNQYMKNKDIEQDEQNKEITKNTADEEILNFDLIEDEKTTEFNVEDNNVKK" }, "paralog": false, "identity": 0.9599109131403119, "score": 859.0, "evalue": 1.8e-317, "description": "Internal stop codon at 165759. Nonsense mutation around 165759.", "psc": { "uniref90_id": "UniRef90_UPI00215D9F07", "uniref50_id": "UniRef50_UPI00215D9F07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D9F07", "UniRef:UniRef50_UPI00215D9F07" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9F07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9F07" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 40586.80060000005, "isoelectric_point": 4.097602653503418 }, "id": "DFOKDJOJIG_346", "locus": "GCA013367855_00311" }, { "type": "cds", "sequence": "contig_1", "start": 166951, "stop": 167202, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATAAAGATATTGGAACTAAAATTGCAAAGCAAAAAAAGATTAAAAAAATTTTGTTCATAACTTTTTCTGTTGTTGGTATTGCTACAATCGTAGGAGCAACAATTGGAGTTACAAGCAGAAAAAAAATGGAATTAGTAACTTATGATGATTACATTGATGTTTCTCTATATTCACAAATAAATTCTAAATTTAAAGAATTGTATCTTGACGGAAAACAACCTGACAAAAAAAATTACCCAATTACTTGA", "aa": "MHKDIGTKIAKQKKIKKILFITFSVVGIATIVGATIGVTSRKKMELVTYDDYIDVSLYSQINSKFKELYLDGKQPDKKNYPIT", "aa_hexdigest": "16337a4af01fbfeaa76c4db496e5ab46", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9428.019099999994, "isoelectric_point": 9.663955879211429 }, "id": "DFOKDJOJIG_347", "locus": "GCA013367855_00312" }, { "type": "cds", "sequence": "contig_1", "start": 167812, "stop": 168069, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGAAGATTGAAAAAAATGAAAATAATATTTTTACTAAGATGCTCAATTTAGAATTCAAGTCAACTTTTTATACTATTGTAAAGAATAAATTAATTTTAAAAAATAAATCTAGTTATGAATATGCTATTTATGTTCCATTTTTTACAAATAAGGATAAGACCATTAAAGACGGCAAGGAAATTTATCAACCTAGAGTTTTTGAATATAGTTGTCCTATAACATTTGTTAATAGTAATTACAAAAAATTTAAATAA", "aa": "MKKIEKNENNIFTKMLNLEFKSTFYTIVKNKLILKNKSSYEYAIYVPFFTNKDKTIKDGKEIYQPRVFEYSCPITFVNSNYKKFK", "aa_hexdigest": "63080570052b2df715a084222eb9d4f1", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10285.906799999995, "isoelectric_point": 9.656799888610841 }, "id": "DFOKDJOJIG_348", "locus": "GCA013367855_00313" }, { "type": "cds", "sequence": "contig_1", "start": 168131, "stop": 168658, "strand": "-", "frame": 3, "gene": "def", "product": "peptide deformylase", "db_xrefs": [ "COG:COG0242", "COG:J", "EC:3.5.1.88", "GO:0036211", "GO:0042586", "KEGG:K01462", "SO:0001217", "UniRef:UniRef50_Q98PN3", "UniRef:UniRef90_D1J7P7" ], "nt": "ATGTTTAAATTTGAAGATGTTAAATTAGTTCAATTACCAGAAAAAGTATTGAGAAATAAATCAAAGAACGTTGAACTCCCATTGACCGCAGAAGATGATTTATTAATACAAAAAATGATTTTCCATGTTGATGATAGTCAAACTCCTCAAACAAAATTTAGACCTGCCGTTGGAGTTGCAGCAGTTCAATATGGAATATTGAAAAATATTTTTTATGTAATGGTAAAAGACGATCAAAATAATTATATTTTTAGAGACGCTTTAATAAATCCAATAATGCTTTCACATTCAAACCATAAAATATCATTGCAAGAAGGGGAGGGATGTTTAAGTGTTAAGGAAAGCGATGCAGGTCAAGAAGGTTTTGTATATAGATATTCAAGAGTTGTAATAAAAGCATATTCGTATTTTGAAAAAAAAGAAAAAATTTATGATGTTTCCGGCTATCCGGCAATTGTATTGCAACATGAATATGACCACTTACAAGGAAAATTATTTATAGATAGAATCAATAAAAATCAACCTTGA", "aa": "MFKFEDVKLVQLPEKVLRNKSKNVELPLTAEDDLLIQKMIFHVDDSQTPQTKFRPAVGVAAVQYGILKNIFYVMVKDDQNNYIFRDALINPIMLSHSNHKISLQEGEGCLSVKESDAGQEGFVYRYSRVVIKAYSYFEKKEKIYDVSGYPAIVLQHEYDHLQGKLFIDRINKNQP", "aa_hexdigest": "4356b018542dd2d3bbac879a184fc64a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7P7", "query_cov": 1.0, "subject_cov": 0.9459459459459459, "identity": 0.9940000000000001, "score": 346.0, "evalue": 2.35e-120, "valid": true, "gene": "def", "product": "peptide deformylase", "ec_ids": [ "3.5.1.88" ], "uniref50_id": "UniRef50_Q98PN3", "cog_id": "COG0242", "cog_category": "J", "kegg_orthology_id": "K01462", "go_ids": [ "GO:0036211", "GO:0042586" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN3" ], "product": "Peptide deformylase" }, "genes": [ "def" ], "id": "DFOKDJOJIG_349", "locus": "GCA013367855_00314" }, { "type": "cds", "sequence": "contig_1", "start": 168937, "stop": 169212, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGATTGAAAAAAAGCAAAAGTTCGCTAAATTAATTCAAAAACAACATTTATTTTTTAATAGAATTTTAGGGACTATTTTAATAGCTAATAATTTGGTAAATATTGCATCTGCAACATTGTTTTCATATGTTTTATCATTAACAACAATGTCTTCTGGCAAGGCTACTATTATTTCAACTGCTGTTATGACTCCACTTATTGTTTTGTTTGCAGAAATTTTACCAAAATTAATTGCAAAAGCATATCCTGAAAGGGTTATTAGATCAACTTGTTGA", "aa": "MIEKKQKFAKLIQKQHLFFNRILGTILIANNLVNIASATLFSYVLSLTTMSSGKATIISTAVMTPLIVLFAEILPKLIAKAYPERVIRSTC", "aa_hexdigest": "7766a89e40dcf13447ec1cdff86791b7", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 88, "aa_cov": 0.967032967032967, "hmm_cov": 1.0, "evalue": 1.0006251678627793e-19, "score": 66.57260131835938, "start": 2, "stop": 89 } ], "seq_stats": { "molecular_weight": 10111.135399999988, "isoelectric_point": 10.17938060760498 }, "id": "DFOKDJOJIG_350", "locus": "GCA013367855_00315" }, { "type": "cds", "sequence": "contig_1", "start": 169306, "stop": 169848, "strand": "+", "frame": 1, "gene": "mpfA", "product": "Hemolysin C", "db_xrefs": [ "COG:COG1253", "COG:PT", "SO:0001217", "UniRef:UniRef50_A0A077L6Q7", "UniRef:UniRef90_D1J7P8" ], "nt": "ATGAAAAACTTGATTAATATTGCACAAGATGAAGGTGTTTTAGAAATCAACGAATCAACTATGGCAAAGAATGTTTTGGATCTTGATTCAAGCAAGGTTTCTCAAAATTACGTTAAGTTAAAAGATATTGATTATTTGAATTATAAAGCAACTTTGCAAGATGCATTAAACAAGTTTAAAGAAACTAATTATTCAAGATTGCCAATTGAAAAAGATGGAGATATGATAGGCATATTGCTTTTAAAAGATATATTCTTTTTAAAGCGTGGCAATATTATGAATTATGTTAAAACGGTGCCAAAAATTTCTGCAAATAGTTTACTTTCTATTGCATTAGAAAAATTAAGAAAAGATAGAGCACAAATGGGTTTCGTTATTAAAAATAATAATGATGACAGTGTCATAGGAATAATTACAATCGAAGATATTATTGAAGAAGTTGTAGGCGAAATATATGACGAATATGATGATGATGAACAAATTTATGAAATAAGTTTGGAACGCTGTGAGGCCCGCGGAAACGTTAAAATTTCAACAATTTGA", "aa": "MKNLINIAQDEGVLEINESTMAKNVLDLDSSKVSQNYVKLKDIDYLNYKATLQDALNKFKETNYSRLPIEKDGDMIGILLLKDIFFLKRGNIMNYVKTVPKISANSLLSIALEKLRKDRAQMGFVIKNNNDDSVIGIITIEDIIEEVVGEIYDEYDDDEQIYEISLERCEARGNVKISTI", "aa_hexdigest": "30080c9a45c3eb04e114bc23cde7f5bb", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "mpfA" ], "truncated": "both", "pseudogene": { "start": 169006, "stop": 170055, "observations": { "mutation": [ 169210, 169231, 169846, 169861, 169918, 170044 ], "stop": [ 169210, 169231, 169846, 169861, 169918, 170044 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P8", "query_cov": 1.0, "subject_cov": 0.4147465437788018, "identity": 0.9940000000000001, "score": 340.0, "evalue": 2.5e-114, "gene_start": 1, "gene_end": 180, "reference_start": 186, "reference_end": 365, "reference_sequence": "MPEPLSGNYNTLNSASGASTSTGAPWQYILYSIILFLLILFSAIFSASETAYTTVSRAKIENMIEKKQKFAKLIQKQHLFFNRILGTILIANNLVNIASATLFSYVLSLTTMSSGKATIISTAVMTPLIVLFAEILPKLIAKAYPERVIRSTCWFIEALYWIFFPITYPISKIGKKIYITNTEEDMKNLINIAQDEGVLEINESTMAKNVLDLDSSKVSQNYVKLKDIDYLNYKATLQDALNKFKETNYSRLPIEKDGDMIGILLLKDIFFLKRGNIMNYVKTVPKISANSLLSIALEKLRKARAQMGFVIKNNNDDSVIGIITIEDIIEEVVGEIYDEYDDDEQIYEISLERCEARGNVKISTIWKRFEWDEILNLEAPNDEDLNLSNWLKNRVGHELHVNTKYTYEDKIDFQVISLKNKDQKFDIIDIDWSK" }, "paralog": false, "identity": 0.9799426934097422, "score": 652.0, "evalue": 1.63e-239, "description": "Internal stop codon at 169210, 169231, 169846, 169861, 169918, 170044. Nonsense mutation around 169210, 169231, 169846, 169861, 169918, 170044.", "psc": { "uniref90_id": "UniRef90_D1J7P8", "gene": "MpfA", "product": "Hemolysin C", "uniref50_id": "UniRef50_A0A077L6Q7", "cog_id": "COG1253", "cog_category": "PT", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P8", "UniRef:UniRef50_A0A077L6Q7", "COG:COG1253", "COG:PT" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L6Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L6Q7" ], "product": "Hemolysin C" } }, "id": "DFOKDJOJIG_351", "locus": "GCA013367855_00316" }, { "type": "cds", "sequence": "contig_1", "start": 170230, "stop": 170670, "strand": "+", "frame": 1, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "KEGG:K02990", "RefSeq:WP_012855389.1", "SO:0001217", "UniParc:UPI0001BC20BC", "UniRef:UniRef100_D1J7P9", "UniRef:UniRef50_B3PN60", "UniRef:UniRef90_D1J7P9" ], "nt": "ATGTCAAATTACGAAATTATGATTCTAACTAATCCAAAAACAACAGATGAAGAATTACAAAATCTAGTATTTTCAGTATTAGATAAAAATTCATCAAAATTTGAAAGATTAGAAAGAAACGAAACTGCATATCCAATTAAAAAGTTAACTCATGTAAATTACTTTTTAATTACAACAAAGGCAGAACCTCATCTAATGTCTGAATTAACAAGAAAGCTTAATATTGATAAATCAGTTTTAAGATCTTTAATTATTAATTTAGATTCTGAAAAAGGTTTAAAGCCAAGAAAAGTTTCTAAGAAATTCAATAAGAGAATTCCTCTAAACCGTGAAAATAAAAAACCTTATGTAAAAAACAACGAAACTTCTAAAGATGCTAGCGAAGAAAAGAAGGTTGCAAGAAAACCAAGAACTTCAAAAGTTTTAGACGAAAAGAAATAG", "aa": "MSNYEIMILTNPKTTDEELQNLVFSVLDKNSSKFERLERNETAYPIKKLTHVNYFLITTKAEPHLMSELTRKLNIDKSVLRSLIINLDSEKGLKPRKVSKKFNKRIPLNRENKKPYVKNNETSKDASEEKKVARKPRTSKVLDEKK", "aa_hexdigest": "1f6220519f0b35fdeb0c010b1e0bdf54", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20BC", "ncbi_nrp_id": "WP_012855389.1", "uniref100_id": "UniRef100_D1J7P9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20BC", "RefSeq:WP_012855389.1", "UniRef:UniRef100_D1J7P9" ] }, "ips": { "uniref100_id": "UniRef100_D1J7P9", "uniref90_id": "UniRef90_D1J7P9" }, "psc": { "uniref90_id": "UniRef90_D1J7P9", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_B3PN60", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN60" ], "product": "Small ribosomal subunit protein bS6" }, "genes": [ "rpsF" ], "id": "DFOKDJOJIG_352", "locus": "GCA013367855_00317" }, { "type": "cds", "sequence": "contig_1", "start": 170699, "stop": 170857, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00436.31" ], "nt": "ATGAATAAAGTATTTTTAATTGGTCGTTTGTCGGCCAAACCTTATAAAAATGTTACACCTTCTAATGTTGAATATTCAAGATTTACTATGGCTATTAGACGCGAATACGCCGCCCCTAATTCTGAACCTGTAGTAGACTTTGTCCCATGTGTTGCTTGA", "aa": "MNKVFLIGRLSAKPYKNVTPSNVEYSRFTMAIRREYAAPNSEPVVDFVPCVA", "aa_hexdigest": "e3643d89a9a5ba85e9b34a3c195bc11e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00436.31", "name": "SSB", "length": 54, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.429386396434073e-09, "score": 32.86436462402344, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5877.7719000000025, "isoelectric_point": 9.426970100402833 }, "id": "DFOKDJOJIG_353", "locus": "GCA013367855_00318" }, { "type": "cds", "sequence": "contig_1", "start": 171017, "stop": 171157, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAAAATAGACGAAAAAATAACAATGGTAAAGAATTTAATATTGCCACAGAAGAATCAAACACTATCGATAATAATTTAGAAAATAATCATCAATCAAATGAATCAAATGATAATCCTTTTGATCAACTTGATTGA", "aa": "MSENRRKNNNGKEFNIATEESNTIDNNLENNHQSNESNDNPFDQLD", "aa_hexdigest": "27e71f40877bf2643a03d5e9efb89bb6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5352.431800000002, "isoelectric_point": 4.394188499450684 }, "id": "DFOKDJOJIG_354", "locus": "GCA013367855_00319" }, { "type": "cds", "sequence": "contig_1", "start": 171175, "stop": 171474, "strand": "+", "frame": 1, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "RefSeq:WP_012855391.1", "SO:0001217", "UniParc:UPI0001BC20BE", "UniRef:UniRef100_D1J7Q1", "UniRef:UniRef50_D1J7Q1", "UniRef:UniRef90_D1J7Q1" ], "nt": "ATGGCAAGAATTGTTCGTAAGAAACCTTTTATTCGCAAACGTCCATGTCAATTTTGTTTAAGTAAAGAACCAGTTGTTTACATTGACTACAAAAATGAAGAAGTTTTATCAAAATTAGTAAATTTACAAGGAAAAATTTTATCATCACGTATTACCGGAACATGTGCAAAACACCAAAGAGCAGTTGCATTAGCAATTAAAAGAGCAAGATTTGTTGGTATTTTGCCATACATCGGTTTAGTAAAAAGAGAATCTAAAAAAGAAACTGTTAAAACTGAAAAAGTAGAAAAAGCAGAATAA", "aa": "MARIVRKKPFIRKRPCQFCLSKEPVVYIDYKNEEVLSKLVNLQGKILSSRITGTCAKHQRAVALAIKRARFVGILPYIGLVKRESKKETVKTEKVEKAE", "aa_hexdigest": "8ab7f0f5f7af77f919b7787eca37ee67", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20BE", "ncbi_nrp_id": "WP_012855391.1", "uniref100_id": "UniRef100_D1J7Q1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20BE", "RefSeq:WP_012855391.1", "UniRef:UniRef100_D1J7Q1" ] }, "ips": { "uniref100_id": "UniRef100_D1J7Q1", "uniref90_id": "UniRef90_D1J7Q1" }, "psc": { "uniref90_id": "UniRef90_D1J7Q1", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_D1J7Q1", "cog_id": "COG0238", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Q1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Q1" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "DFOKDJOJIG_355", "locus": "GCA013367855_00320" }, { "type": "cds", "sequence": "contig_1", "start": 172310, "stop": 173038, "strand": "+", "frame": 2, "gene": "rarA", "product": "ATPase, AAA family", "db_xrefs": [ "COG:COG2256", "COG:L", "KEGG:K07478", "SO:0001217", "UniRef:UniRef50_A0A077L8I4", "UniRef:UniRef90_D1J7Q3" ], "nt": "ATGGATTCTAACTTAGCAAATTTAATAAGGCCCAAAATTTTAGAAGATTTTATTTGCCCACAAGAACAAAAAAAATTATTTCAAAAAATTATAGAAAATAATGATTATCGTTCATTTATTTTTTACGGAAAGCCAGGTACTGGAAAAACAACTATTTCCTATATTCTTGCCAATGCTTTAAAAGTAAATTACGAATATTTTAATGCGGCCATTGAAAATAAAGAAGATCTTGTACATAAATTAAAGTTGAACAAGATTTTGATCATTGATGAGATTCATAGATTAAACAAAGATAAACAAGATATATTGCTACCATATCTAGAAAACGATTTAATCACAATTTATGCAACTACAACGGAAAATCCATATTTTAAGCTTAATCCTGCATTACGTTCAAGATGTTCAATAATTGAAATTACTAAGCCAACAATTCAAGATTTATCAAGTTGTTTAAAAAATGCCTGCCTTAAAACTTTTGGTGTGGATCTTAATAAGGATATTGCTGATTATTTGGCCTTGCAGGCTAATGGCGACTTTAGATCGGCATTAAACAATCTTGATTTGATTATGTTAATTTTTAAAGATCAAGAATTTTCATTGGAGGCAATTAAAAAGATTATTCCCTCAATTCAATTTAGTTCAGATGAATCTGGCGATAATCATTATGATTATTTAAGTGCTTTTCATAAATCACTACGCGGTTCGGACATTGATGCAGCATTATATTGA", "aa": "MDSNLANLIRPKILEDFICPQEQKKLFQKIIENNDYRSFIFYGKPGTGKTTISYILANALKVNYEYFNAAIENKEDLVHKLKLNKILIIDEIHRLNKDKQDILLPYLENDLITIYATTTENPYFKLNPALRSRCSIIEITKPTIQDLSSCLKNACLKTFGVDLNKDIADYLALQANGDFRSALNNLDLIMLIFKDQEFSLEAIKKIIPSIQFSSDESGDNHYDYLSAFHKSLRGSDIDAALY", "aa_hexdigest": "9da47514d867137624f354b080ba7fbc", "start_type": "ATG", "rbs_motif": null, "genes": [ "rarA" ], "truncated": "3-prime", "pseudogene": { "start": 172310, "stop": 173341, "observations": { "mutation": [ 173036 ], "stop": [ 173036 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q3", "query_cov": 1.0, "subject_cov": 0.599009900990099, "identity": 1.0, "score": 482.0, "evalue": 1.43e-169, "gene_start": 1, "gene_end": 242, "reference_start": 1, "reference_end": 242, "reference_sequence": "MDSNLANLIRPKILEDFICPQEQKKLFQKIIENNDYRSFIFYGKPGTGKTTISYILANALKVNYEYFNAAIENKEDLVHKLKLNKILIIDEIHRLNKDKQDILLPYLENDLITIYATTTENPYFKLNPALRSRCSIIEITKPTIQDLSSCLKNACLKTFGVDLNKDIADYLALQANGDFRSALNNLDLIMLIFKDQEFSLEAIKKIIPSIQFSSDESGDNHYDYLSAFHKSLRGSDIDAALYWGFIIIKSGDFDGLFRRMLCASYEDVGMANPKLGSEVLSAIECFERIGMPEGYLPITQAILNICLSPKSNTVYSSSTLIKSIIDSGNIFEVPIHLKDAHYKSAAKLKRGLNYKYPHDYKNNYIDQQYLPNELKDKIHIELNGQGYEQKIKQYWDLIKNKGEK" }, "paralog": false, "identity": 0.9970845481049563, "score": 680.0, "evalue": 4.77e-250, "description": "Internal stop codon at 173036. Nonsense mutation around 173036.", "psc": { "uniref90_id": "UniRef90_D1J7Q3", "gene": "RarA", "product": "ATPase, AAA family", "uniref50_id": "UniRef50_A0A077L8I4", "cog_id": "COG2256", "cog_category": "L", "kegg_orthology_id": "K07478", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q3", "UniRef:UniRef50_A0A077L8I4", "COG:COG2256", "COG:L", "KEGG:K07478" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L8I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L8I4" ], "product": "ATPase" } }, "id": "DFOKDJOJIG_356", "locus": "GCA013367855_00321" }, { "type": "cds", "sequence": "contig_1", "start": 173075, "stop": 173494, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12002.13" ], "nt": "TTGTTTAGAAGAATGTTGTGTGCAAGTTACGAAGATGTTGGAATGGCAAATCCTAAATTGGGATCAGAGGTTTTGTCGGCAATTGAATGTTTTGAAAGAATAGGAATGCCTGAGGGGTATTTACCAATAACACAAGCAATATTAAATATTTGTTTAAGTCCAAAATCAAACACTGTTTATAGTTCATCAACATTAATAAAATCTATAATTGACAGTGGAAATATTTTTGAAGTGCCAATTCATTTAAAAGATGCTCATTATAAATCGGCAGCAAAACTAAAAAGAGGATTAAATTATAAATATCCTCATGATTATAAAAATAATTATATTGATCAACAATATCTACCAAATGAATTAAAAGATAAAATTCATATTGAATTAAATGGGCAAGGTTATGAACAAAAAATTAAACAATATTGA", "aa": "MFRRMLCASYEDVGMANPKLGSEVLSAIECFERIGMPEGYLPITQAILNICLSPKSNTVYSSSTLIKSIIDSGNIFEVPIHLKDAHYKSAAKLKRGLNYKYPHDYKNNYIDQQYLPNELKDKIHIELNGQGYEQKIKQY", "aa_hexdigest": "3c6fda4fe81798fb9208ff8e42ca8689", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12002.13", "name": "MgsA_C", "length": 137, "aa_cov": 0.9856115107913669, "hmm_cov": 1.0, "evalue": 4.2761002128437284e-45, "score": 149.2564239501953, "start": 2, "stop": 138 } ], "seq_stats": { "molecular_weight": 15923.146799999979, "isoelectric_point": 8.34403018951416 }, "id": "DFOKDJOJIG_357", "locus": "GCA013367855_00322" }, { "type": "cds", "sequence": "contig_1", "start": 173526, "stop": 173843, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02912.23" ], "nt": "ATGGAATTTGATTTAAATAAAATAAATACATTGGAAGATTTAAAGTTAGCTAAAAATGCTTTTAACAATTCAGATGAGTTAAAAGAATTAATGTCTCAAATTAAAACAGCAGATCCATTGCAAAAAGCAGAACTTGGTAAAAAAATTCAACTGTTAAAAAAGCAAGCAGAAGATTTTTTTGATTTAGCAAAGCAAAAACTTGAAGATATAAAAATTCAAGATTTAATTAATCAAGAGTATGTAGATTTTGCAAATCCTATAAATACAACCGGAGGAATTCATCCAATTAATTTGGTTGCAAATAGATTTAGAGAATGA", "aa": "MEFDLNKINTLEDLKLAKNAFNNSDELKELMSQIKTADPLQKAELGKKIQLLKKQAEDFFDLAKQKLEDIKIQDLINQEYVDFANPINTTGGIHPINLVANRFRE", "aa_hexdigest": "399641ab99a851063a0cfdba480224fb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02912.23", "name": "Phe_tRNA-synt_N", "length": 64, "aa_cov": 0.6, "hmm_cov": 1.0, "evalue": 4.8245468842724656e-08, "score": 28.557003021240234, "start": 11, "stop": 73 } ], "seq_stats": { "molecular_weight": 12088.696599999985, "isoelectric_point": 5.212385749816893 }, "id": "DFOKDJOJIG_358", "locus": "GCA013367855_00323" }, { "type": "cds", "sequence": "contig_1", "start": 173847, "stop": 174284, "strand": "+", "frame": 3, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "COG:COG0016", "COG:J", "EC:6.1.1.20", "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_C5J6V7", "UniRef:UniRef90_D1J7Q4" ], "nt": "TTGGCAAATGGATATTTTGAATATACAGGGCCAGAAATAGAAAATGAAGAATATAACTTTGAACGTTTGAATATTCCTCAATCTCACCCTGCAAGAGAGATGCAAGATTCACTATATATATCAGAAAAAGAATTGCTTAGAACTCACAATACGGGATTTAGTGCAAGGGCATTAGAAGAACATAAAAATCAAGAGTTCTCACAATTTGCTATTGGAAAAGTTTATAGAAATGATGAAGAAGATAGAACACATACTCACCAATTTACTCAATTAGATTTTGTTAGTGTTGGCAACCATTCATTTGGAACATTGATTTATACATTGACTGAATTATTTTCTTATGTTTTAGAACAAAAGGTAAAAATTAGACTACGTCCTTCTTATTTCCCATTTACTGAACCTAGTGTTGAGGTTGATGTTTTGTTTAATGAAAAATGA", "aa": "MANGYFEYTGPEIENEEYNFERLNIPQSHPAREMQDSLYISEKELLRTHNTGFSARALEEHKNQEFSQFAIGKVYRNDEEDRTHTHQFTQLDFVSVGNHSFGTLIYTLTELFSYVLEQKVKIRLRPSYFPFTEPSVEVDVLFNEK", "aa_hexdigest": "440bb23b0ffb9648cd4f870c67eeb395", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "pheS" ], "truncated": "both", "pseudogene": { "start": 173547, "stop": 174467, "observations": { "mutation": [ 173841, 174282 ], "stop": [ 173841, 174282 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q4", "query_cov": 1.0, "subject_cov": 0.46325878594249204, "identity": 0.993, "score": 290.0, "evalue": 6.57e-97, "gene_start": 1, "gene_end": 145, "reference_start": 108, "reference_end": 252, "reference_sequence": "MEFDLNKINTLEDLKLAKNAFNNSDELKELMSQIKTADPLQKAELGKKIQLLKKQAEDFFDLAKQKLEDIKIQDLINQEYVDFANPINTTGGIHPINLVANRFREWLLANGYFEYTGPEIENEEYNFERLNIPQSHPAREMQDSLYISEKELLRTHNTGFSARALEEHKNQEFSQFAIGKVYRNDEEDRTHTHQFTQLDFVSVGNHSFGTLIYTLTELFSYVLEQKVKIRLRPSYFPFTEPSVEVDVLFNEKWVEVLGAGMLHENVLKMAGYTNDMNGIAAGIGIERITMIKYGIDDIRELYGNDQRFLKQFK" }, "paralog": false, "identity": 0.9934640522875817, "score": 604.0, "evalue": 3.56e-223, "description": "Internal stop codon at 173841, 174282. Nonsense mutation around 173841, 174282.", "psc": { "uniref90_id": "UniRef90_D1J7Q4", "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_C5J6V7", "cog_id": "COG0016", "cog_category": "J", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q4", "EC:6.1.1.20", "UniRef:UniRef50_C5J6V7", "COG:COG0016", "COG:J", "KEGG:K01889", "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_C5J6V7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C5J6V7" ], "product": "Phenylalanine--tRNA ligase alpha subunit" } }, "id": "DFOKDJOJIG_359", "locus": "GCA013367855_00324" }, { "type": "cds", "sequence": "contig_1", "start": 174306, "stop": 174467, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGTTGCATGAAAATGTTTTAAAAATGGCCGGATATACCAATGATATGAATGGCATTGCTGCAGGAATTGGTATTGAAAGAATAACAATGATAAAGTATGGCATTGACGATATAAGAGAATTGTATGGAAATGACCAAAGATTTTTAAAACAATTTAAATAA", "aa": "MLHENVLKMAGYTNDMNGIAAGIGIERITMIKYGIDDIRELYGNDQRFLKQFK", "aa_hexdigest": "247ce24247d8c02fcba2a60b9e877202", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 52, "aa_cov": 0.9811320754716981, "hmm_cov": 1.0, "evalue": 1.103163396483163e-19, "score": 66.50789642333984, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 6107.0264, "isoelectric_point": 6.533664894104004 }, "id": "DFOKDJOJIG_360", "locus": "GCA013367855_00325" }, { "type": "cds", "sequence": "contig_1", "start": 174479, "stop": 175192, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGTTATTTTCTTATAAAAAAATAGTTAAATTGGCAAATATAACTGATAAAAGTGTAGAAGAGGTTGTTGGAGCAATTAATTCAATAGGCTTTGAAGTTGAAGAATATCATAAGTTTTTAGATGCTGAAGGTATGAAATTCTGTCATGTATTAAAAGCATACAAAAATCCTAATTCTGATCGTTTAACGGTTTGTGAAGTTGAATTTGGCAATGGAAATCATTCAATAATTCAAACTAATGCAACAAACATGCATGAGGGCGATTATGTAATGGCTTATGTTCCTGGATCGAGATCTGGAAAAAATATATTTGCTGCCAAGGAATTAAAAGGAATCATCTCTGAAGGAATGTTTGTAAGTTTGAATGAAATTGGTATTCCAGAAGAATTTCATCCCTTAGATTCAAAAGATGGTATTTTTCAAGTTGGAAAAATAGATTTAAATTTAGACCCTGTTGAATATTTTGATCTTGATGATTATTTGATTGATGTAACAATTTTATCCAATAGAGCTGATGCTTTGTGCTATTTAATTTTAGCTAAAGAAATTTCAGCATATTTTGAATCTTATATTGAACCTCTTTCAAAACCAAAACCTACCTTGGAAAGCAATATTGTTGTAAGCAAAAATTTAGTTGCTACAAACTGTTTTAGTTTAGTTGAAGCTATTACTAAACAACCTGCTATTTCTCTTCAAGATGAATTTCTTTTATGA", "aa": "MLFSYKKIVKLANITDKSVEEVVGAINSIGFEVEEYHKFLDAEGMKFCHVLKAYKNPNSDRLTVCEVEFGNGNHSIIQTNATNMHEGDYVMAYVPGSRSGKNIFAAKELKGIISEGMFVSLNEIGIPEEFHPLDSKDGIFQVGKIDLNLDPVEYFDLDDYLIDVTILSNRADALCYLILAKEISAYFESYIEPLSKPKPTLESNIVVSKNLVATNCFSLVEAITKQPAISLQDEFLL", "aa_hexdigest": "de88bd3490aae3b5698502f05a59994e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI001F47B76D", "query_cov": 1.0126582278481013, "subject_cov": 1.00418410041841, "identity": 0.504, "score": 223.0, "evalue": 7.71e-70, "valid": false, "uniref50_id": "UniRef50_A0AAV4HE63" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 82, "aa_cov": 0.3291139240506329, "hmm_cov": 0.9146341463414634, "evalue": 1.987234470248405e-09, "score": 33.136253356933594, "start": 46, "stop": 123 } ], "seq_stats": { "molecular_weight": 26460.973900000034, "isoelectric_point": 4.757219123840332 }, "id": "DFOKDJOJIG_361", "locus": "GCA013367855_00326" }, { "type": "cds", "sequence": "contig_1", "start": 175238, "stop": 176617, "strand": "+", "frame": 2, "gene": "eMAP", "product": "phenylalanine--tRNA ligase subunit beta", "db_xrefs": [ "COG:COG0073", "COG:J", "EC:6.1.1.20", "KEGG:K01890", "SO:0001217", "UniRef:UniRef50_A0A448ZZ89", "UniRef:UniRef90_D1J7Q5" ], "nt": "TTGGTCTTATTATACGCAGGAGTTCCTTGTCACGTATATAATAAAAATTCTTTAAGTTCAAATGAGTTTTCTACATCATTGAGTAATGAAACATTAAATGTTTTAGGCAATAAACAGGTTACATTAAACAACAATTTGGTTGTAAAAAACGGAGATCAAACCGTTTCAATAGCGAGTGTTATAGGCTTAGAAAATTATGAATATAGACCTGATGATGGACATTGCGTCTTTGAATTAGCATCATTTTCATTAAAAGAAGTTAGAAAAAGTATAAAAACAGTCAAATTAGAAACGTTAGCATCTTTACGTGGTTCAAAAGAAATATCAAGCGGTCAAGTAGTTATGGCATATGAATTTTTAACTAAATATTTAGATCAATATTCAGTTCAAATAAACGCGCCTAAGCTAAGAAAAACAAATATATTAATTGATAGCCATTATATTACTAAATATGCAGGCTTCAATATTACAAAAACAAAAAAATATATTCAAGTTTTGAATCAATTGACTCTTTTGGGCTTTAAAATAAAGGAAGATTTATCATCAGTAATTTTTCCAACTTATAGATACGATTTAAAAAATATGCAAGATTTTGTTGAAGAAGTGTTTAGATTTTATGGATATGATAATTTCCCAGCTAAATCTCCAAAAATTACTAGATTATTAATTAAAGAAAACAAAGAATTTAAGTTTATTGAAAACTTCTGTGCAAAAGGATATTTCAATGTTAGAACATACACGTTAATTAATCCTGAAAAAAACATTTTTAATCCATTTGATTTTAAAACTAATTTCAATGCAATTGCAGCAAAGAATTATGATCATTCTCAAATTAGAAATTCAATGATTTCTTCATTATTAGAAGTCTTGGATACAAATATTAAACAAGGTTTGCCAGACGCTTCATATTTTGAAATAGCTATGATTAATAATAAAATGAATGTATTAGGAATTGCTTCATTAAATAAAACTATTGATGATATAAAAGCTGATATTTGTTCAATTACTAATGAAATATTAGAATTTAAACCAAGTAAATTAAAAATTTTTAATCCAAATGCAGCACAAGAAATTTATTTAAATAACGAGTTAATTGGATATGTTGCAAAATTAGACCCTCACTATTGTCAATATAATGCAGTGTTTAGTGAAGTCTTTTTGGACAAAATGAATAGTAATGTAATTAAATATAAAAATTATAAACATACACCTCTTAAGTCTAGAGATGTTACTATATCTGTTTTAAATAAACAATCTATTGAAGAAACAATTAAAAAAATACACAGTATTTTTGGCGTGTACAAAATTTCAATTAAGGATGTTTATAATAAAGATAATGGCACAAAAAATATTACATTAGAAGTAATTTTAGAAGATTGA", "aa": "MVLLYAGVPCHVYNKNSLSSNEFSTSLSNETLNVLGNKQVTLNNNLVVKNGDQTVSIASVIGLENYEYRPDDGHCVFELASFSLKEVRKSIKTVKLETLASLRGSKEISSGQVVMAYEFLTKYLDQYSVQINAPKLRKTNILIDSHYITKYAGFNITKTKKYIQVLNQLTLLGFKIKEDLSSVIFPTYRYDLKNMQDFVEEVFRFYGYDNFPAKSPKITRLLIKENKEFKFIENFCAKGYFNVRTYTLINPEKNIFNPFDFKTNFNAIAAKNYDHSQIRNSMISSLLEVLDTNIKQGLPDASYFEIAMINNKMNVLGIASLNKTIDDIKADICSITNEILEFKPSKLKIFNPNAAQEIYLNNELIGYVAKLDPHYCQYNAVFSEVFLDKMNSNVIKYKNYKHTPLKSRDVTISVLNKQSIEETIKKIHSIFGVYKISIKDVYNKDNGTKNITLEVILED", "aa_hexdigest": "f3c57a78e0f1ad4b4f69f3801d3b12d8", "start_type": "TTG", "rbs_motif": null, "genes": [ "eMAP" ], "truncated": "both", "pseudogene": { "start": 174938, "stop": 176653, "observations": { "mutation": [ 175190, 176615 ], "stop": [ 175190, 176615 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q5", "query_cov": 1.0, "subject_cov": 0.6339779005524862, "identity": 0.9890000000000001, "score": 891.0, "evalue": 0.0, "gene_start": 1, "gene_end": 459, "reference_start": 254, "reference_end": 712, "reference_sequence": "MLFSYKKIVKLANITDKSVEEVVGAINSIGFEVEEYHKFLDAEGMKFCHVLKAYKNPNSDRLTVCEVEFGNGNHSIIQTNATNMHEGDYVMAYVPGSRSGKNIFAAKELKGIISEGMFVSLNEIGIPEEFHPLDSKDGIFQVGKIDLNLDPVEYFDLDDYLIDVTILSNRADALCYLILAKEISAYFESYIEPLSKPKPTLESNIVVSKNLVATNCFSLVEAITKQPAISLQDEFLLWKHGIKTFRNAVDITNLVLLYAGVPCHVYNKNSLSSNEFSTSLSNETLNVLGNKQVTLNNNLVVKNGDQTVSIASVIGLENYEYRPDGGHCVFELASFSLKEVRKSIKTVKLETLASLRGSKEISSGQVVMAYEFLTKYLDQYSVQINAPKLRKTNILIDSHYITKYAGFNITKTKKYIQVLNQLTLLGFKIKEDLSSVIFPTYRYDLKNMQDFVEEVFRFYGYDNFPAKSPKITRLLIKENKEFKFIENFCAKGYFNVRTYTLINPEKNIFNPFDFKTNFNAIAAKNYDHSQIRNSMISSLLEVLDTNIKQGLPDASYFEIAMINNKMNVLGIASLNKTIDDIKADICSITNETLEFKPSKLKIFNPNAAQEIYLNNELIGYVAKLDPHYCQYNAVFSEVFLDKMNSNVIKYKNYKHTPLKSRDVTISVLDKQSIEETIKKIQSIFGVYKISIKDVYNKDNGTKNITLEVILEDWATKKFDSIFNK" }, "paralog": false, "identity": 0.989492119089317, "score": 1110.0, "evalue": 0.0, "description": "Internal stop codon at 175190, 176615. Nonsense mutation around 175190, 176615.", "psc": { "uniref90_id": "UniRef90_D1J7Q5", "gene": "EMAP", "product": "phenylalanine--tRNA ligase subunit beta", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_A0A448ZZ89", "cog_id": "COG0073", "cog_category": "J", "kegg_orthology_id": "K01890", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q5", "EC:6.1.1.20", "UniRef:UniRef50_A0A448ZZ89", "COG:COG0073", "COG:J", "KEGG:K01890" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZ89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZ89" ], "product": "Phenylalanine--tRNA ligase beta subunit" } }, "id": "DFOKDJOJIG_362", "locus": "GCA013367855_00327" }, { "type": "cds", "sequence": "contig_1", "start": 176751, "stop": 176936, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAACAACAAATGATAATCTTCAAAAAGAAAAGAGCATTATTGCTGAAACATTAAAGGAAATAGAAAAGAAATTCGGCAAAGAAGCCGTTATGATTTTAGGAGAAAAGCCAAGCATAATTCCTGAAACTTTTAGCAGTGGTTCGTTGACTATCGATAGAGCGTTAGGAATAAATGGATGA", "aa": "MKETTNDNLQKEKSIIAETLKEIEKKFGKEAVMILGEKPSIIPETFSSGSLTIDRALGING", "aa_hexdigest": "c9f41f4d8b6240cde5449c81972c0dfd", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6693.649199999999, "isoelectric_point": 5.342262840270995 }, "id": "DFOKDJOJIG_363", "locus": "GCA013367855_00328" }, { "type": "cds", "sequence": "contig_1", "start": 176997, "stop": 177638, "strand": "+", "frame": 3, "gene": "recA", "product": "Protein RecA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9RV21" ], "nt": "TTGCACGCAATTGCTGAAATACAAAAAACTGGCGGAATTGCAGCATTTATTGATGCTGAGCATTCTATAGATCCAATATATGCCAAAAATTTAGGGGTTGATATTGACAATTTAATTTTATCTCAACCAGATTCAGGAGAACAAGCATTAGAAATTGTTGATACATTGTCTAAATCCGGAGCAATAGGACTAATCGTTGTTGATTCGGTTGCTGCATTGGTTCCTGAGGCTGAGTTGAATGGAGAAATGAAAGACCAAGTAGTAGGTGCACAGGCAAGGCTTATGTCTAAAGCTTTAAGAAAAATAACTGGATCATTATCAAAAAATAAGACAACAGTAATATTTATTAATCAAATAAGAGAAAAAATTGGAGTTATTTTTGGCAATCCTGAAACAACAACTGGCGGAAGAGCATTGAAATTTTATTCAAGCATCCGTCTTGAAGTTAGAAAAATTCAAAATGTAACCAATAATGGAGACATCACAGGTAATCAAATTAAGTGTAAAGTTGTAAAGAACAAATTAGCTGCTCCATATAAAACTGCTCAAATTGATATTATTTTTTCAAAAGGTATTTCAAAAGAGCTTGAAATAATTAATTTAGCAGAAGAATTAGGAATTTTAGTAAAAAAAGGCTCGTGA", "aa": "MHAIAEIQKTGGIAAFIDAEHSIDPIYAKNLGVDIDNLILSQPDSGEQALEIVDTLSKSGAIGLIVVDSVAALVPEAELNGEMKDQVVGAQARLMSKALRKITGSLSKNKTTVIFINQIREKIGVIFGNPETTTGGRALKFYSSIRLEVRKIQNVTNNGDITGNQIKCKVVKNKLAAPYKTAQIDIIFSKGISKELEIINLAEELGILVKKGS", "aa_hexdigest": "b0f1378336773daef7e2d17b28aa8210", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A0D5BUH9", "query_cov": 0.9765258215962441, "subject_cov": 0.9244444444444444, "identity": 0.625, "score": 266.0, "evalue": 9.97e-88, "valid": false, "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_A0A7U9RV21", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9RV21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9RV21" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "DFOKDJOJIG_364", "locus": "GCA013367855_00329" }, { "type": "cds", "sequence": "contig_1", "start": 177804, "stop": 178028, "strand": "+", "frame": 3, "gene": null, "product": "Metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "nt": "ATGAAAAATGAAAATATAAAAATTCTATTTTTGGGAGACATATTTGGTTTACCGGGAATTAGATTTGTTGAGCAACAATTGCCAAACATAATAAAACAAAATAATATTGATTTTGTAATCGCTCAAGCAGAAAACGTTTCGGGAAGAAAGGGTTTTGAGCCTAAAGATTACGAAAGATTGAAAAGAGCGGGAATAAATGCATTTACACTAGGAAATCACGTTTGA", "aa": "MKNENIKILFLGDIFGLPGIRFVEQQLPNIIKQNNIDFVIAQAENVSGRKGFEPKDYERLKRAGINAFTLGNHV", "aa_hexdigest": "d1de8a61c0e753d54d0f0f87de7e0890", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FBC5", "query_cov": 0.9324324324324325, "subject_cov": 1.0, "identity": 0.536, "score": 89.0, "evalue": 6.56e-22, "valid": false, "product": "Metallophosphoesterase", "uniref50_id": "UniRef50_A0A432FBC5" }, "pscc": { "uniref50_id": "UniRef50_A0A432FBC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "product": "Metallophosphoesterase" }, "genes": [], "id": "DFOKDJOJIG_365", "locus": "GCA013367855_00330" }, { "type": "cds", "sequence": "contig_1", "start": 178389, "stop": 178610, "strand": "+", "frame": 3, "gene": null, "product": "Putative metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PKR0" ], "nt": "TTGCCTAAGGGTACATATTTTGTAACTGATGCCGGAATGTGTGGCCCAAGAGATTGCGCTATTGGTTCTGACTTCCAAGAAGTATATGAAAAAATGCGTTACGCAGCATTTTCTGCATTTAAGGTATCAAAAAATAAATGTCAATTAAATGGAGTTATTTTTACATTAACCAAGGATCCAGCAGAAAAGCATATTGAGTTAGTAAATATAAAGGAGCAATAA", "aa": "MPKGTYFVTDAGMCGPRDCAIGSDFQEVYEKMRYAAFSAFKVSKNKCQLNGVIFTLTKDPAEKHIELVNIKEQ", "aa_hexdigest": "8282ca45faaa513dd9bb80f277f0eab0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PKR0", "query_cov": 0.9863013698630136, "subject_cov": 0.9863013698630136, "identity": 0.6809999999999999, "score": 108.0, "evalue": 1.66e-29, "valid": false, "product": "Putative metallophosphoesterase", "uniref50_id": "UniRef50_A0A3B0PKR0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PKR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PKR0" ], "product": "Putative metallophosphoesterase" }, "genes": [], "id": "DFOKDJOJIG_366", "locus": "GCA013367855_00331" }, { "type": "cds", "sequence": "contig_1", "start": 178612, "stop": 178872, "strand": "+", "frame": 1, "gene": "nusB", "product": "N utilization substance B-like protein(Protein nusB)", "db_xrefs": [ "COG:COG0781", "COG:K", "SO:0001217", "UniRef:UniRef50_D1J7Q8", "UniRef:UniRef90_D1J7Q8" ], "nt": "ATGAATAACGATTCATCATCAAAAATCAGTGAGAAGGATAAACGAATTTTATTTCTACTCAATAAATATAAGGCAAGGATGCATATTATTAATTGCATATATCAGAGCGAGCTATTTAATAAGGATCTTTCAATACAAGAAATTTTGGAAAATTGTGGAGAAAATCAATTGAATGCGAATGAAATAGAATCTTTAAAAATAATTGAAGCTAAATACGATGTTTTTACTAGAATAGCAAAAAAATTTATTAGTGAAGACTGA", "aa": "MNNDSSSKISEKDKRILFLLNKYKARMHIINCIYQSELFNKDLSIQEILENCGENQLNANEIESLKIIEAKYDVFTRIAKKFISED", "aa_hexdigest": "b40b5389667a39fa05e4df71e197f566", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "nusB" ], "truncated": "3-prime", "pseudogene": { "start": 178612, "stop": 179085, "observations": { "mutation": [ 178870, 178876 ], "stop": [ 178870, 178876 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q8", "query_cov": 1.0, "subject_cov": 0.5477707006369427, "identity": 0.988, "score": 164.0, "evalue": 1.42e-50, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MNNDSSSKISEKDKRILFLLNKYKARMHIINCIYQSELFNKNLSIQEILENCGENQLNANEIESLKIIEAKYDVFTRIAKKFISEDWEWNRILPLSRAIIIYGEYELLFNDPRVVINEMVNISKIYSPNNDYKFINKVLDLVSKNVIKKNTKDEKEN" }, "paralog": false, "identity": 0.9808917197452229, "score": 301.0, "evalue": 9.04e-107, "description": "Internal stop codon at 178870, 178876. Nonsense mutation around 178870, 178876.", "psc": { "uniref90_id": "UniRef90_D1J7Q8", "gene": "NusB", "product": "N utilization substance protein B homolog(Protein nusB)", "uniref50_id": "UniRef50_D1J7Q8", "cog_id": "COG0781", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q8", "UniRef:UniRef50_D1J7Q8", "COG:COG0781", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Q8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Q8" ], "product": "N utilization substance protein B homolog(Protein nusB)" } }, "id": "DFOKDJOJIG_367", "locus": "GCA013367855_00332" }, { "type": "cds", "sequence": "contig_1", "start": 178966, "stop": 179085, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAATATTTCAAAAATATACAGTCCTAATAATGATTATAAATTTATCAATAAGGTTCTAGATCTTGTTTCTAAAAATGTAATTAAAAAAAATACTAAAGATGAAAAAGAAAATTAG", "aa": "MVNISKIYSPNNDYKFINKVLDLVSKNVIKKNTKDEKEN", "aa_hexdigest": "348a48353fc4b62036a2aff9bc7366ba", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4585.2389, "isoelectric_point": 9.399055290222169 }, "id": "DFOKDJOJIG_368", "locus": "GCA013367855_00333" }, { "type": "cds", "sequence": "contig_1", "start": 179069, "stop": 179806, "strand": "+", "frame": 2, "gene": "yqxC", "product": "Hemolysin A", "db_xrefs": [ "COG:COG1189", "COG:J", "EC:2.1.1.226", "EC:2.1.1.227", "KEGG:K06442", "RefSeq:WP_175972223.1", "SO:0001217", "UniParc:UPI00158DBFC4", "UniRef:UniRef100_UPI00158DBFC4", "UniRef:UniRef50_A0A077LAY8", "UniRef:UniRef90_D1J7Q9" ], "nt": "ATGAAAAAGAAAATTAGCGAGATAATAACCAATATTTTTAAATACGATTTGAAGATTTCAGAAGCGTTAATTAGAGAAGGAAGAGTAATTGTAAATAATGAAAAGATAATTATTCCATCATTAAAATTTGAAGAAACCGATGTAGATATAAGATTCATTAATATGAAATTGCCCTATGTTTCAAGAGGCGCTTATAAATTATTAGAAGCAATAGAAAAATTTAATATAAACTTAACGGATAAAATATGTCTTGACATAGGATCGTCTACTGGGGGATTTGTTGATGTAATGTTAAGGCATGGTGCTAAAAAAGTATATGCCGTAGATTGTGGCACAAATCAACTAGCATATGAACTTAGGATAAATGATAAGGTTGTTGTTTATGAAAAAACTAATCTTAAAAATTTAAAAAAGGAAATGTTCCTTGAAAATATTGAATTTGTATCTTGTGATGTTTCATTTATAAGCTTGAAATATGTATTTGAGGTTTGCAATAAAATATTAAATAATAATAATATAAATGTAGTTTTTTTAATTAAACCCCAATTTGAAGCTTCAAGCAAATATGTTGAACCCGGAGGATATGTTAATCAAGAAAATCATAAATATATTATTGATAAAGTAATTTCATATGCAAAAAATAATGGCTTTGTTTTGATTGATTTAATAAAAAGCCCTATTTTAGGAGAAAAATCAAAAAATATTGAATATTTAGGCTTTTTTAGAAAGGATAGTTAA", "aa": "MKKKISEIITNIFKYDLKISEALIREGRVIVNNEKIIIPSLKFEETDVDIRFINMKLPYVSRGAYKLLEAIEKFNINLTDKICLDIGSSTGGFVDVMLRHGAKKVYAVDCGTNQLAYELRINDKVVVYEKTNLKNLKKEMFLENIEFVSCDVSFISLKYVFEVCNKILNNNNINVVFLIKPQFEASSKYVEPGGYVNQENHKYIIDKVISYAKNNGFVLIDLIKSPILGEKSKNIEYLGFFRKDS", "aa_hexdigest": "af42b510febae3fcc01e1931e28eb1fa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158DBFC4", "ncbi_nrp_id": "WP_175972223.1", "uniref100_id": "UniRef100_UPI00158DBFC4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158DBFC4", "RefSeq:WP_175972223.1", "UniRef:UniRef100_UPI00158DBFC4" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158DBFC4", "uniref90_id": "UniRef90_D1J7Q9" }, "psc": { "uniref90_id": "UniRef90_D1J7Q9", "gene": "YqxC", "product": "Hemolysin A", "ec_ids": [ "2.1.1.226", "2.1.1.227" ], "uniref50_id": "UniRef50_A0A077LAY8", "cog_id": "COG1189", "cog_category": "J", "kegg_orthology_id": "K06442" }, "pscc": { "uniref50_id": "UniRef50_A0A077LAY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LAY8" ], "product": "Hemolysin A" }, "genes": [ "yqxC" ], "id": "DFOKDJOJIG_369", "locus": "GCA013367855_00334" }, { "type": "cds", "sequence": "contig_1", "start": 179806, "stop": 180498, "strand": "+", "frame": 1, "gene": "csdA", "product": "Nitrogen fixation protein NifS(Aminotransferase protein S)", "db_xrefs": [ "COG:COG0520", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZVE8", "UniRef:UniRef90_D1J7R0" ], "nt": "ATGAATTATAAGGATTTATTTCCTATGTTTAAAAATAACCCTGGTATTGTATATTTCGATAATTCAGCACTTACTTTTAAACCGCAAACCGTAATAAATTCAGGTATTGATTTTTATGAAAAATATTCAATTTCTACAAGGACTGCTGATAGTGTTTTAGGAATACAAACAGAAGAAGTAATGCATGAAACTAGAAAAAAAATTGCAGATTTGATTGACGCTTCTGCCGATCAAACTATATTTACTTCAGGAACGACTGATGGATTAAACTTAATATCTTTAATGTTAAAAGATATAATAAAGGATGGCGAAATAATATTTAGCTATTTTAATCACAGCTCAGCAATCGTTCCATTCATTGAAAATCTAAAAAACAATAATATAACATTTAAATATGCGAAAGATAACGAGGAAGTTTTAGATTTAATAAATGAAAAAACGAAGTTAATTGTATTGCCTCAATTAACTAATAATTTTAATGTATTTTTTGATTTAAAAAAAATTCATCAAAAATCTAAAAAATATGGTGCATATTTGATAAACGATGCCGCACAAGCTATTGCTCACGAAAAAGTTTCATTGAACGACTGCGATGTAATTGTTTTTTCAAGTAATAAAATTTATGGTCCTACCGGTATGGGAGCTTTAGTAATTGAAAAAGATTTACTAAAAAAAATAGTTCCTCAAAAATGA", "aa": "MNYKDLFPMFKNNPGIVYFDNSALTFKPQTVINSGIDFYEKYSISTRTADSVLGIQTEEVMHETRKKIADLIDASADQTIFTSGTTDGLNLISLMLKDIIKDGEIIFSYFNHSSAIVPFIENLKNNNITFKYAKDNEEVLDLINEKTKLIVLPQLTNNFNVFFDLKKIHQKSKKYGAYLINDAAQAIAHEKVSLNDCDVIVFSSNKIYGPTGMGALVIEKDLLKKIVPQK", "aa_hexdigest": "41d7d379d2c52ff35cd02ac2e9d71ce2", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "csdA" ], "truncated": "3-prime", "pseudogene": { "start": 179806, "stop": 180801, "observations": { "mutation": [ 180496, 180538 ], "stop": [ 180496, 180538 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7R0", "query_cov": 1.0, "subject_cov": 0.5912596401028277, "identity": 0.983, "score": 446.0, "evalue": 7.31e-156, "gene_start": 1, "gene_end": 230, "reference_start": 1, "reference_end": 230, "reference_sequence": "MNYKDLFPMFKNNPGIVYFDNSALTFKPQTVINSGIDFYEKYSISTRTADSVLGIQTEEVIHETRKKIADLIDASANQTIFTSGTTDGLNLISLMLKDIIKDGEIIFSYFNHSSAIVPFIENLKNNNITFKYAKDNEEVLDLINEKTRLIVLPQLTNNFNVFFDLKKIHQKSKKYGAYLINDAAQAIAHEKVSLNDCDVIVFSSNKIYGPTGMGALVIEKDLLEKIVPQKWGGGQVQSIGSGCEWSAKNDIKKFEPGTPNFAGIFQFNAAIDFINSLGYELINKLETAVANYCYDELKTIDGIEIDSKRGDKIILFNIKNIPSQDIASYLGHNNIYVRSGVFCAHKFHELKQYSNSYVRISISFYNDYNDVDKFIKLLKEMGANFLEFL" }, "paralog": false, "identity": 0.9818731117824774, "score": 644.0, "evalue": 2.47e-236, "description": "Internal stop codon at 180496, 180538. Nonsense mutation around 180496, 180538.", "psc": { "uniref90_id": "UniRef90_D1J7R0", "gene": "CsdA", "product": "Nitrogen fixation protein NifS(Aminotransferase protein S)", "uniref50_id": "UniRef50_A0A448ZVE8", "cog_id": "COG0520", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7R0", "UniRef:UniRef50_A0A448ZVE8", "COG:COG0520", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVE8" ], "product": "Probable cysteine desulfurase" } }, "id": "DFOKDJOJIG_370", "locus": "GCA013367855_00335" }, { "type": "cds", "sequence": "contig_1", "start": 180962, "stop": 181411, "strand": "+", "frame": 2, "gene": "iscU", "product": "Aminotransferase protein U-like protein (Nitrogenfixation protein NifU)", "db_xrefs": [ "COG:COG0822", "COG:O", "RefSeq:WP_175972224.1", "SO:0001217", "UniParc:UPI00158A74C3", "UniRef:UniRef100_UPI00158A74C3", "UniRef:UniRef50_D1J7R1", "UniRef:UniRef90_D1J7R1" ], "nt": "TTGAATTTTTATAGTAATATTGAAAAACAAAGAATAATTTTTGAAGCATACGAAAACCCAAAATTTAAAATAAAAAATGATGAAGATTTTAAGAAAACTGATAAGACATTATTTGAACATTCAAATGTATGCGTTGACGATATTAAGTTAAATTTTTTATTCGATGAAAAAAATACTTTGAAAAAAGTGGAATATTGTGCTAAAGGGTGTTCAATTTTTTTGTCATCCGTTGAATTAATGATTCAGCAAATTTTGAATAAAAATAAATTAGAAATAAATAAAATTTTAAATGAATATAAAACAATGATAGATACATCTACAATTTCACAACGAGGTGGAGAAATATTGGGAAAATTGGTTGTATATCAAAATGTAAGAGTTCATTTAAATAGGTTAGAATGTGCATCAATAATTTTTAGGTTAATAAAAAAAGCAGTTTCTAATGAATAA", "aa": "MNFYSNIEKQRIIFEAYENPKFKIKNDEDFKKTDKTLFEHSNVCVDDIKLNFLFDEKNTLKKVEYCAKGCSIFLSSVELMIQQILNKNKLEINKILNEYKTMIDTSTISQRGGEILGKLVVYQNVRVHLNRLECASIIFRLIKKAVSNE", "aa_hexdigest": "6434843b6f2fd2f5fec8abc5e0043e5c", "start_type": "TTG", "rbs_motif": "GGxGG", "ups": { "uniparc_id": "UPI00158A74C3", "ncbi_nrp_id": "WP_175972224.1", "uniref100_id": "UniRef100_UPI00158A74C3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158A74C3", "RefSeq:WP_175972224.1", "UniRef:UniRef100_UPI00158A74C3" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158A74C3", "uniref90_id": "UniRef90_D1J7R1" }, "psc": { "uniref90_id": "UniRef90_D1J7R1", "gene": "IscU", "product": "Aminotransferase protein U homolog (Nitrogenfixation protein NifU)", "uniref50_id": "UniRef50_D1J7R1", "cog_id": "COG0822", "cog_category": "O" }, "pscc": { "uniref50_id": "UniRef50_D1J7R1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7R1" ], "product": "Aminotransferase protein U homolog (Nitrogenfixation protein NifU)" }, "genes": [ "iscU" ], "id": "DFOKDJOJIG_371", "locus": "GCA013367855_00336" }, { "type": "cds", "sequence": "contig_1", "start": 181404, "stop": 182297, "strand": "+", "frame": 3, "gene": "dinP", "product": "DNA-damage repair protein MucB", "db_xrefs": [ "COG:COG0389", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J7R2", "UniRef:UniRef90_D1J7R2" ], "nt": "ATGAATAAGATAATTTTTCACATAGATATGGACTCCTTCTTTGTTAGTTGTGAACGTTCTATTAATACAAACCTGATAGACAAGCCTGTTGTAATTGCTAAACGATACAAACGTTCAATAATTAGTGCAATGTCTTACGAGGTAAAAAACCTTGGATATAGAGTAGGAAGTTCTTTATACACCGTTTTAGAAAAGGTTCCTAATTTACTTATAGTAGAACCACACTATGATTTATATTCTTTAATTTCTAAAAAAATTTTTGATTATTTAAAAAATAAGTATTGTACACAACTAGAAATATATTCAATAGACGAATGCTATATTGATTTTAGCAACACTGTTAGTAATTGGGAGGAAGCTAAAAATATTGCCCAACAAATTAAAGATGATATTTTAAAAACATTTATGATTCCTTGCTCAATAGGAATTTCCTTTACAAAGTTTTTGGCAAAAATGAGTACAAATAAAGCAAAACCATCAGGAATTTCAATAACAAAAAAGGAAGATATTGAAAGAAATTTTTACTCATTGCCGGTGGAAAAAATATTTGGGGTAGGAAAAAATATTTCGCAACAATTAATAAAAAATAGAATTCTTAATTATAAAGATTTGCTAGAATGCAATAATGAAATTTTTTTAAGAAAATTGTTTAAAAAAAATTTTTTCTTATTTATTGAACAACTAAAGGGAATCAATGCAACCGACCACATCTTAAATCTTGAAACAAAAGGAATAAGTAACACGATAACTTTTATGGAAAAAGATTCTGATGATATCGTTTTTTTGAAAAATGAATTGTTTAAATTGGCAAGAAATGTTGCTAATAGGGCCGTAAACAACAATTATGAATGCAGATGTGTTGAAGTTTTAATAAGAAAACAAGACAAAAAATGA", "aa": "MNKIIFHIDMDSFFVSCERSINTNLIDKPVVIAKRYKRSIISAMSYEVKNLGYRVGSSLYTVLEKVPNLLIVEPHYDLYSLISKKIFDYLKNKYCTQLEIYSIDECYIDFSNTVSNWEEAKNIAQQIKDDILKTFMIPCSIGISFTKFLAKMSTNKAKPSGISITKKEDIERNFYSLPVEKIFGVGKNISQQLIKNRILNYKDLLECNNEIFLRKLFKKNFFLFIEQLKGINATDHILNLETKGISNTITFMEKDSDDIVFLKNELFKLARNVANRAVNNNYECRCVEVLIRKQDKK", "aa_hexdigest": "4b008ce84e296156302d0c2e5d20c638", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "dinP", "mucB" ], "truncated": "3-prime", "pseudogene": { "start": 181404, "stop": 182600, "observations": { "mutation": [ 182295, 182382 ], "stop": [ 182295, 182382 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7R2", "query_cov": 1.0, "subject_cov": 0.7105263157894737, "identity": 0.976, "score": 575.0, "evalue": 3.19e-205, "gene_start": 1, "gene_end": 297, "reference_start": 1, "reference_end": 297, "reference_sequence": "MNKIIFHIDMDSFFVSCERSINTNLIDKPVVIAKRYKRSIISAMSYEVKNLGYRVGSSLYTVLEKVPNLLIVEPHYDLYSLISKKIFDYLKNKYCTQLEIYSIDECYIDFSNTVSNWAEAKNLAQQIKDDILKTFMIPCSIGISFTKFLAKMSTNKAKPSGILITKKGDIERNFYSLPVEKIFGVGKNISQQLIKNKISTYKDLLECNNEIFLRKLFKKNFFLFIEQLKGINATDHILNLETKGISNTITFMEKDSDDIVFLKNELFKLARNVANRAVNNNYECRCVEVLIRKQDKKWMSEQKKIDFFTNDIDIIFKIALKLFLNLWNEKECLRGLGIRICDLRSIFQENKQVNLFEDNNQKSIVNSILNEVNTKFEGKILITGKQYLLENSIDIDNIKFLRKNNQPYDKKINLEEKK" }, "paralog": false, "identity": 0.9748743718592965, "score": 765.0, "evalue": 1.09e-282, "description": "Internal stop codon at 182295, 182382. Nonsense mutation around 182295, 182382.", "psc": { "uniref90_id": "UniRef90_D1J7R2", "gene": "DinP", "product": "DNA-damage repair protein MucB", "uniref50_id": "UniRef50_D1J7R2", "cog_id": "COG0389", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7R2", "UniRef:UniRef50_D1J7R2", "COG:COG0389", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7R2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7R2" ], "product": "DNA-damage repair protein MucB" } }, "id": "DFOKDJOJIG_372", "locus": "GCA013367855_00337" }, { "type": "cds", "sequence": "contig_1", "start": 182430, "stop": 182660, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGTCGATATTTCAAGAAAACAAACAAGTTAATTTGTTTGAAGACAATAATCAAAAATCAATAGTAAATTCAATATTAAATGAGGTTAATACAAAATTTGAGGGTAAAATTTTAATAACAGGGAAACAATATTTGTTAGAGAATTCAATTGATATTGATAATATAAAGTTTTTAAGAAAAAATAATCAACCCTATGATAAAAAAATAAATTTAGAGGAGAAAAAATAA", "aa": "MRSIFQENKQVNLFEDNNQKSIVNSILNEVNTKFEGKILITGKQYLLENSIDIDNIKFLRKNNQPYDKKINLEEKK", "aa_hexdigest": "53be2b029bc8566ca5d7d8503c3332c4", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9030.19379999999, "isoelectric_point": 8.925663948059086 }, "id": "DFOKDJOJIG_373", "locus": "GCA013367855_00338" }, { "type": "cds", "sequence": "contig_1", "start": 182660, "stop": 183001, "strand": "+", "frame": 2, "gene": null, "product": "nicotinate-nucleotide adenylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "nt": "ATGAAGATAGGAATATTTGGCGGAAGTTTTGATCCTGTTCATAAAGGACATATTTTAATTGCTAATGATGCAATTGAATTATTAAAACTGGATGAAGTTATTTTTGTTCCTGCTAACAAAAATCCTTTTAAAGATAAGCAAGATTATGCATCTAATGAACATAGAATAAATATGATTAATATTGTTATAAACAAGAGTAATATGGAAGTTTCACAATTTGAAACAAAAAGAGGTGGCACTTCATACACAATTGATACTGTAAAATATTTTGCTCAAAAGTATCCAAACGATGAGCTATATTTTTTAATTGGATCGGATAATGTTGGTAGTTTAAACAAATGA", "aa": "MKIGIFGGSFDPVHKGHILIANDAIELLKLDEVIFVPANKNPFKDKQDYASNEHRINMINIVINKSNMEVSQFETKRGGTSYTIDTVKYFAQKYPNDELYFLIGSDNVGSLNK", "aa_hexdigest": "5382f7ce6c4a2423e4c5747a9a26c6dc", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FBE0", "query_cov": 1.008849557522124, "subject_cov": 1.0178571428571428, "identity": 0.579, "score": 129.0, "evalue": 1.07e-36, "valid": false, "gene": "nadD", "product": "nicotinate (nicotinamide) nucleotide adenylyltransferase", "ec_ids": [ "2.7.7.18" ], "uniref50_id": "UniRef50_A0A432FBE0", "go_ids": [ "GO:0000309", "GO:0004515", "GO:0005737", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FBE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "product": "nicotinate-nucleotide adenylyltransferase" }, "genes": [], "id": "DFOKDJOJIG_374", "locus": "GCA013367855_00339" }, { "type": "cds", "sequence": "contig_1", "start": 183175, "stop": 183363, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCACTCTTTAGTAATATCATGCATAAAACCAGTTCTATACGCTAATTCGACATCAAAATTATTTCTTTTAGCCAATTGTGCAGAAAATTCAGCAGTATATCTTAAATGTTTAAATCTAGGAATAGAAAGAGTATTTTTGGCAATATTTTCTAAATATAAACAATTCTTTCCTATGTATTCTTGA", "aa": "MSHSLVISCIKPVLYANSTSKLFLLANCAENSAVYLKCLNLGIERVFLAIFSKYKQFFPMYS", "aa_hexdigest": "8c6032f08446de5a4be592873d9b2f5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7027.318199999999, "isoelectric_point": 9.183988761901855 }, "id": "DFOKDJOJIG_375", "locus": "GCA013367855_00340" }, { "type": "cds", "sequence": "contig_1", "start": 183425, "stop": 183589, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGCACCAAACTACGGCCTATTATTGGTTGAGGGATGTATATAAATATCAAAATGATGAAGCGTTACAAGCAATCAAAATTCATACATCATTAGATTTTAAAATATCTTTATTGGCAAAAATTTTATATATTGCTGACAAAATTTGTGAAGGAAGAAAATGA", "aa": "MLHQTTAYYWLRDVYKYQNDEALQAIKIHTSLDFKISLLAKILYIADKICEGRK", "aa_hexdigest": "bd5763502d0e2b7603ea2617461d9da3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6434.442399999998, "isoelectric_point": 8.788862037658692 }, "id": "DFOKDJOJIG_376", "locus": "GCA013367855_00341" }, { "type": "cds", "sequence": "contig_1", "start": 183808, "stop": 184497, "strand": "+", "frame": 1, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0006413", "RefSeq:WP_020002689.1", "SO:0001217", "UniParc:UPI0003616783", "UniRef:UniRef100_UPI0003616783", "UniRef:UniRef50_A0A448ZXX5", "UniRef:UniRef90_D1J7R4" ], "nt": "TTGCTGGGTTGCTACAATAAAGAATTTGTAGTTTTTGGGAAGTGGATTAATACCACTTTTTTATTTATAAAAGAAGGAGGAGTTATTATTAATAACCAAAAAATTGGAGACGATTTAAATAACAATCAAACAAATCGTAAACCAAAGTCAGAACATGTTATCAACAATGCTATTCCATACAAAAAAGTTTTTGTTCTAGGACCTACAGGGGAAAAAATTGGTGTGTTAACCAAAGAAGAAGCTTTAGAAAAAGCATCTGAATATAATATGGATTTAGTTTTAATTGCAATAGAAAACAATAAGCCAATAGTTAGAATATTAGATTATGGCAAATTTAAATATGATAAGAAGAAACGCCAAAAAGAAGTTAAAGAAAAACAAGCAGTTACTGTTAACAGAGAAATTCGCTTAACTCCATTGATTGGTGATCATGATTTGAAAACGAAGGCAAACAAAACTAGAGAATTTATTTTAGATGGTAATAGAGTTAAAGTTTCAGTTAAGTTCCGTGGTAGAGAAAGAGCAAGAATTGAATTAGGGGAAGAAATATTAAAAAAGTTCTTTGCCTTAATTGAAGATGTTGCAAAAATTTCAAAAGAAGCAACATTAGTTAATGATAGATTTTTAGATATGTATGTAGAAAAAGATAAACGAAAAGTTGATACATTAACCAATAAAAAAGATAACTAA", "aa": "MLGCYNKEFVVFGKWINTTFLFIKEGGVIINNQKIGDDLNNNQTNRKPKSEHVINNAIPYKKVFVLGPTGEKIGVLTKEEALEKASEYNMDLVLIAIENNKPIVRILDYGKFKYDKKKRQKEVKEKQAVTVNREIRLTPLIGDHDLKTKANKTREFILDGNRVKVSVKFRGRERARIELGEEILKKFFALIEDVAKISKEATLVNDRFLDMYVEKDKRKVDTLTNKKDN", "aa_hexdigest": "19f4657fcbf6cdce778424ebb9cdfd02", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003616783", "ncbi_nrp_id": "WP_020002689.1", "uniref100_id": "UniRef100_UPI0003616783", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003616783", "RefSeq:WP_020002689.1", "UniRef:UniRef100_UPI0003616783" ] }, "ips": { "uniref100_id": "UniRef100_UPI0003616783", "uniref90_id": "UniRef90_D1J7R4" }, "psc": { "uniref90_id": "UniRef90_D1J7R4", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_A0A448ZXX5", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXX5" ], "product": "Translation initiation factor IF-3" }, "genes": [ "infC" ], "id": "DFOKDJOJIG_377", "locus": "GCA013367855_00342" }, { "type": "cds", "sequence": "contig_1", "start": 184524, "stop": 184712, "strand": "+", "frame": 3, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_020002688.1", "SO:0001217", "UniParc:UPI00036BF9D9", "UniRef:UniRef100_A0A2K9YT13", "UniRef:UniRef50_P75447", "UniRef:UniRef90_D1J7R5" ], "nt": "ATGCCAAAAATGAAAACCAAAAGCGGTTTGAAAAAAAGAATTTCAATCACTGCATCAGGAAAAGTAAAACGTGGACAAGCGTTTAGATCACATTTAGCACAAAACAAAACAACAAAACAAAAACGTCACGCTCGTAAAGCAACAACTTTATCAAGTTCAGATTTTAAAAGATATAAAGAATTAATTTAG", "aa": "MPKMKTKSGLKKRISITASGKVKRGQAFRSHLAQNKTTKQKRHARKATTLSSSDFKRYKELI", "aa_hexdigest": "12cf694fb7c510f41385e8bf4b0e3d29", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00036BF9D9", "ncbi_nrp_id": "WP_020002688.1", "uniref100_id": "UniRef100_A0A2K9YT13", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036BF9D9", "RefSeq:WP_020002688.1", "UniRef:UniRef100_A0A2K9YT13" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YT13", "uniref90_id": "UniRef90_D1J7R5" }, "psc": { "uniref90_id": "UniRef90_D1J7R5", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_P75447", "cog_id": "COG0291", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P75447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75447" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "DFOKDJOJIG_378", "locus": "GCA013367855_00343" }, { "type": "cds", "sequence": "contig_1", "start": 185183, "stop": 185293, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGAAAAAAAGAGTGGTATTAGGAATGAGTGGCGGAGTTGATTCTAGTGTTTGTGGATATTTGTTGCAAAAACAAGGATATGACGTTGTTGGTTTGTTCATGAGAAATTGA", "aa": "MKKRVVLGMSGGVDSSVCGYLLQKQGYDVVGLFMRN", "aa_hexdigest": "09399c4b20bbd243650b061f6885b900", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 23, "aa_cov": 0.6388888888888888, "hmm_cov": 1.0, "evalue": 1.530641933558558e-07, "score": 26.389699935913086, "start": 3, "stop": 25 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 34, "aa_cov": 0.9444444444444444, "hmm_cov": 1.0, "evalue": 5.314889046344742e-12, "score": 41.443851470947266, "start": 3, "stop": 36 } ], "seq_stats": { "molecular_weight": 3936.6466, "isoelectric_point": 9.514002418518068 }, "id": "DFOKDJOJIG_379", "locus": "GCA013367855_00344" }, { "type": "cds", "sequence": "contig_1", "start": 185579, "stop": 186295, "strand": "+", "frame": 2, "gene": null, "product": "tRNA-uridine 2-sulfurtransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E4E9" ], "nt": "ATGGGGCATTATGCAAATGCAATTGACGGAGAATTATTTCGGGCGCAAGATGATAATAAAGACCAATCTTACTTTTTAGCGCAATTAAGCCCTGGCCAACTTAAAAAAGTAATTTTTCCATTGGCTAATATTACCAAACCAGAAGTAAGAGAAATTGCAAAACAATTAAACTTGTCAACTGCTATGAAAAAAGATTCAACAGGAATTTGCTTTATAGGTGAAAGACACTTCACAAAATTTTTAGAAAATTATATTCCTGCTCAACCAGGTAATATTATTGATATATTTAGCGGGAAAGTGGTTGGAAAACATATTGGTGCAATGTATTACACTATAGGCCAAAGAAAAGGTTTGAATCTTGGCGGGTATGAAAAACCATATTTTGTAGTAGGACATAATCTTTCAAAAAAAGAAATTTATGTAACAAACGATGACCATATTGACTATTTAATGTCAAATAAATTAATTGCAACAAATTTTAATCAAATTTCAAAAGAATTTAATTTTGCTAATTTAACTGCTAAGTTTCGTTATAGACAAAAGGATATCAAAATAACTTTAAAGATTTTAGATGACAATAAAATAGAGGTTTGCTATCCAAATACCGAAGCAGTAACCCCTGGCCAACAAGTTGTAATATATGATGGAAATAAAGTCGTTGGTGGAGCAATAATTGACAAAATTTATTTAAATAATGAATTAAAAACGTTTTTATAA", "aa": "MGHYANAIDGELFRAQDDNKDQSYFLAQLSPGQLKKVIFPLANITKPEVREIAKQLNLSTAMKKDSTGICFIGERHFTKFLENYIPAQPGNIIDIFSGKVVGKHIGAMYYTIGQRKGLNLGGYEKPYFVVGHNLSKKEIYVTNDDHIDYLMSNKLIATNFNQISKEFNFANLTAKFRYRQKDIKITLKILDDNKIEVCYPNTEAVTPGQQVVIYDGNKVVGGAIIDKIYLNNELKTFL", "aa_hexdigest": "38f5731928196a18917bdee15ba52742", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030845807", "query_cov": 0.8235294117647058, "subject_cov": 0.98989898989899, "identity": 0.515, "score": 198.0, "evalue": 1.06e-60, "valid": false, "product": "aminomethyltransferase beta-barrel domain-containing protein", "uniref50_id": "UniRef50_A0A380E4E9" }, "pscc": { "uniref50_id": "UniRef50_A0A380E4E9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E4E9" ], "product": "tRNA-uridine 2-sulfurtransferase" }, "genes": [], "id": "DFOKDJOJIG_380", "locus": "GCA013367855_00345" }, { "type": "cds", "sequence": "contig_1", "start": 186573, "stop": 186719, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGGTAACTTTTCAATAGGCGATTATTTTAAAAAAGAAGCAATTGCTTTTGGATATGAATTTATTTTTAATGTTCTAAAACTAAATAAACAAAATATTTATATAACTTATTATTCAGAAGATAAAGACACTTATAATTATTGA", "aa": "MLGNFSIGDYFKKEAIAFGYEFIFNVLKLNKQNIYITYYSEDKDTYNY", "aa_hexdigest": "ee4df62ca45ebba9d68af99bee4f848e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5793.464500000002, "isoelectric_point": 4.992987251281736 }, "id": "DFOKDJOJIG_381", "locus": "GCA013367855_00346" }, { "type": "cds", "sequence": "contig_1", "start": 187011, "stop": 187889, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATGCTCCAACTAATTTTGATACTGACTTATTTTTACCAATAATTCATGCGACAGAAAAATTAACAAACAAACGTTATGATATTAATAACTATTTTACAAAAGAACCAATTCAAATGAAAATTAACACCCAATTTAAAATAATTGCTGATCACATTAGAGCTTCAGTTAATGCAATAAGTGACGGTGTTAAACCTTCAAATATTGGTCGTGGTTATATTATTAGACGTCTAATAAGAAGAGCATATAGGGCGGGTATTTCTCTTGGCATAACTCAAAAAGCATTTTTATATGAACTTGTTCAAATAGTTAAAGATTCATTAATTTATGACATTGATGTTGAAGAAGTTAAAAAAGTTATTTTGGATGAAGAAAATCTTTTTGCTAAGACTATTAATGAAGGCGAAAAACTATTAAATCAAGAAATTGAAAAAAATGGTACTATCGATGTTAAAATAGCCTTTAAAATGTTTGACACATATGGCTTTCCTATTGAACTAACAAGAGAAATTCTGCAAGAAAAAAACATTGACATCGATATGAATGAATTTAATGAATTTTTAAAGCAACACCAAGAAAAATCAAGAGGAAATATTGAAGCTGGAATGCAAAAGGTTATTAATTCACTTTCATTAATAACTGATAAGATATCTGAATTTGTGGGATATGATTACACTAAAACAACTTCAAAAATTTTATATTTATTAAATAGTGAAGAAAAAATAAATAATGCGGGCGAAGATGAAGTTTCTTATTTAATATTAGATAAAACTCCTTTCTATGCTACAAGTGGTGGCCAAAATCATGATGAAGGTTACATTTTACAAGGGAATAATAGAATTGAAGTTTTAGATGTATTTAAAGACAAATATTGA", "aa": "MQDAPTNFDTDLFLPIIHATEKLTNKRYDINNYFTKEPIQMKINTQFKIIADHIRASVNAISDGVKPSNIGRGYIIRRLIRRAYRAGISLGITQKAFLYELVQIVKDSLIYDIDVEEVKKVILDEENLFAKTINEGEKLLNQEIEKNGTIDVKIAFKMFDTYGFPIELTREILQEKNIDIDMNEFNEFLKQHQEKSRGNIEAGMQKVINSLSLITDKISEFVGYDYTKTTSKILYLLNSEEKINNAGEDEVSYLILDKTPFYATSGGQNHDEGYILQGNNRIEVLDVFKDKY", "aa_hexdigest": "090d3b8272ad6fef330f88e4a504232b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33658.88090000003, "isoelectric_point": 5.166175651550291 }, "id": "DFOKDJOJIG_382", "locus": "GCA013367855_00347" }, { "type": "cds", "sequence": "contig_1", "start": 188001, "stop": 188975, "strand": "+", "frame": 3, "gene": null, "product": "Alanine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZR4" ], "nt": "ATGTTTAGTGCATTAAGAGAAACATATCCAAACGAAATTATCAAACAATTAGGTTCGAATATTACATATGAACATTTTACATTTGATTTCGGTCTTGATCATAGGCCAACTAAAGAAGAGATAAAATTAATTGAACATAAAATGAGGCAATATATTGCTAGAGATGTAAAACGTAATTACATAATAACTTCGCTTAAAAAAGCAGAAGAAATGGGCGCATACATGACAATTGATGAATCAGAATATCATGATGCAAATTATATAAGAGTTGTTGAATTTGAAGGTATTACCAAAGATTTATGTGGTGGAACACACGTTCCTCATTCAGGTATAATTGAAGATTTTAAAATTGTTGCCGTTGATTCAAAAGGTACAGGAATATATAGACTTAGAGTAGTAACTTCATATGATTTGGTAAGAAAATATTTATTATCAGAAATTCAAGAAAAACAAAAAATATTGGATACATTAATTAAAAATAATCAAAAACTAAATGAAGGCTATAACTTTAAATATACAAAGGAAATTATTTCTAATAAGACATCTGATTTAGAAGAATTACTTGAAGAATATGATAAGTGCGAAGAACAATTAAGAAACGATTATCGAGCATTATTAAAACAAAAACAATCAGAAGATGTTGATTTAAGTTTGTATAATTATGAAACCATCAACGATAAAAAAGTATTCTTATTAATTAACATAGAACAATCATTAATTAAAAAAGTTTCAATCTCATTAAGAGAAGCAAATCCTGATTCAATTATTTTTATCTTAAACAAGAGCAAACAAGATTCATATTATTTTGCAGTAGCATCTAAAAAATATGATTCAAATGCAATATTTAAAAACTTGGGCAAGGTTTTAAATATTCATGGCGGTGGAAATGCAATAATTTCTCAAGGTTCATTGGTTTCAAACTTGAAGCAAGAAGAAATTATTGAAATTATTAAGAGCAATTTATGCGAATTTTAG", "aa": "MFSALRETYPNEIIKQLGSNITYEHFTFDFGLDHRPTKEEIKLIEHKMRQYIARDVKRNYIITSLKKAEEMGAYMTIDESEYHDANYIRVVEFEGITKDLCGGTHVPHSGIIEDFKIVAVDSKGTGIYRLRVVTSYDLVRKYLLSEIQEKQKILDTLIKNNQKLNEGYNFKYTKEIISNKTSDLEELLEEYDKCEEQLRNDYRALLKQKQSEDVDLSLYNYETINDKKVFLLINIEQSLIKKVSISLREANPDSIIFILNKSKQDSYYFAVASKKYDSNAIFKNLGKVLNIHGGGNAIISQGSLVSNLKQEEIIEIIKSNLCEF", "aa_hexdigest": "3aba1d6382dcbccbf2b1ce561bdda131", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NZR4", "query_cov": 0.9907407407407407, "subject_cov": 0.9042253521126761, "identity": 0.561, "score": 353.0, "evalue": 3.62e-118, "valid": false, "product": "Alanine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0NZR4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NZR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZR4" ], "product": "Alanine--tRNA ligase" }, "genes": [], "id": "DFOKDJOJIG_383", "locus": "GCA013367855_00348" }, { "type": "cds", "sequence": "contig_1", "start": 188963, "stop": 189310, "strand": "+", "frame": 2, "gene": null, "product": "Putative pre-16S rRNA nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM70" ], "nt": "ATGCGAATTTTAGCATTGGATTTAGGCACAAAAAGTTGTGGCTTTGCAATAAGTGATCCTAATAATATAATTGCAAGTGGACTTGAAAATTTTAGATTTGAATTGAACCATTTTAACTTAGTTATTGAAAAAGTGTTACATTATTTAAACAAAAGTGAATATCAAGGTTCAATTGGCACAATAGTTTTAGGATATCCCTTAAGAATGGATTTATCAAAAAGTCAACGAACGTATATGGTCGAAAGATTTGGAGAAAGATTAAAAAAAGTTGTTGATATTCCAATTGTATTTCAAGATGAAAGACAAAGCACTATTGGAGCTCAAGAAATATTGCAGAGCGCTGGATGA", "aa": "MRILALDLGTKSCGFAISDPNNIIASGLENFRFELNHFNLVIEKVLHYLNKSEYQGSIGTIVLGYPLRMDLSKSQRTYMVERFGERLKKVVDIPIVFQDERQSTIGAQEILQSAG", "aa_hexdigest": "c85741ac8049575c22fafc9172b80e18", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00112C3C79", "query_cov": 1.0, "subject_cov": 0.8098591549295775, "identity": 0.696, "score": 161.0, "evalue": 4.79e-49, "valid": false, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_B3PM70", "go_ids": [ "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM70" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [], "id": "DFOKDJOJIG_384", "locus": "GCA013367855_00349" }, { "type": "cds", "sequence": "contig_1", "start": 189390, "stop": 189701, "strand": "+", "frame": 3, "gene": null, "product": "DUF1292 domain-containing protein", "db_xrefs": [ "RefSeq:WP_012855410.1", "SO:0001217", "UniParc:UPI0001BC20F6", "UniRef:UniRef100_D1J7S0", "UniRef:UniRef50_D1J7S0", "UniRef:UniRef90_D1J7S0" ], "nt": "ATGAATAAAAATATTATTAATTTAGATGTAGTTGATAGGCAATTGACTACAAGCGATGGCGAAAAACTTTATGTTATTTTTGATATTGAAGAAAACGGCGAACATTATCTTGTTTTAACAGATTATGACGCGATTATTTTTGCTAAAGAACAAGATCAAAATTTAATCGAAGTAACAGATGAAGGTGAAATTGACATTCTTGTTGATTTGACGATGGAATTTGCAGAAAATAATTTTGTTTTAGATAAAGATGGAAAATCAGATTTAATGAAGAAATTAATTGGCAACGATCAAGGTGAAAATGAAGCATAA", "aa": "MNKNIINLDVVDRQLTTSDGEKLYVIFDIEENGEHYLVLTDYDAIIFAKEQDQNLIEVTDEGEIDILVDLTMEFAENNFVLDKDGKSDLMKKLIGNDQGENEA", "aa_hexdigest": "85120146fd2db4f4c4dc4a506d74e8d6", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC20F6", "ncbi_nrp_id": "WP_012855410.1", "uniref100_id": "UniRef100_D1J7S0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20F6", "RefSeq:WP_012855410.1", "UniRef:UniRef100_D1J7S0" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S0", "uniref90_id": "UniRef90_D1J7S0" }, "psc": { "uniref90_id": "UniRef90_D1J7S0", "product": "DUF1292 domain-containing protein", "uniref50_id": "UniRef50_D1J7S0" }, "pscc": { "uniref50_id": "UniRef50_D1J7S0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S0" ], "product": "DUF1292 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_385", "locus": "GCA013367855_00350" }, { "type": "cds", "sequence": "contig_1", "start": 189691, "stop": 190410, "strand": "+", "frame": 1, "gene": null, "product": "BC85_0335 family putative methyltransferase", "db_xrefs": [ "RefSeq:WP_175972228.1", "SO:0001217", "UniParc:UPI00158B431E", "UniRef:UniRef100_UPI00158B431E", "UniRef:UniRef50_D1J7S1", "UniRef:UniRef90_D1J7S1" ], "nt": "ATGAAGCATAAGGGTTCAATATTTAGCGCTAATACAAAATTAGGTTTAATAATCTCTGTCGTCGTTGTATTTATTTTAATGTGTGTAATTTTGTTAATAACCATTTTAGCACGTAGACATTTCTTAAAAAGCACAATAACATCAAGCGAAGAACGAGAACTACAAAATTTAAAAACAAAAAATCCAAATTATGGAATTGTTTTGAGTGGCATTAAAAAATATTATGCTAATGTTTTAGGCGACCATTTGATTGCATTTTTAATTAATACAATATATTTGAATAATTTTTTAAACGTTAGTCTTATTGAAAAAAATGATTATGCTTCAATTTCAATAGCAGCATTATCTATGGCTAATGTTTATTCAAAAAATCAATATTTAATTCAAAAGCAAGGTTCAAATATTCAAAACGAAAATCAAGATTTAGATTTATCTAGATTAAATTATGCTGATGAAATTTATAATCCTTCAGATATGATAATATCTTTATGTGATGATCTTTCAATAGAACAACAAATTAATTCAAATTTACCTTATTTAAGTGATAGAGGTATGCTAATTGTTTCATTTAAGACTATAAAAGACTTTAAAAATCAAAAAAATGTTATTTTAGATAATAATTTAAGATACGAAACTGTTAAAATTAGTAATAAAAATGTTATACTTTTAGCAAAAAACAATATGAATTCAAACAATGAAAATTCTAAGGAGCAACAATAA", "aa": "MKHKGSIFSANTKLGLIISVVVVFILMCVILLITILARRHFLKSTITSSEERELQNLKTKNPNYGIVLSGIKKYYANVLGDHLIAFLINTIYLNNFLNVSLIEKNDYASISIAALSMANVYSKNQYLIQKQGSNIQNENQDLDLSRLNYADEIYNPSDMIISLCDDLSIEQQINSNLPYLSDRGMLIVSFKTIKDFKNQKNVILDNNLRYETVKISNKNVILLAKNNMNSNNENSKEQQ", "aa_hexdigest": "3f1b5add03277d257668f3b43f9681dc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158B431E", "ncbi_nrp_id": "WP_175972228.1", "uniref100_id": "UniRef100_UPI00158B431E", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B431E", "RefSeq:WP_175972228.1", "UniRef:UniRef100_UPI00158B431E" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B431E", "product": "BC85_0335 family putative methyltransferase", "uniref90_id": "UniRef90_D1J7S1" }, "psc": { "uniref90_id": "UniRef90_D1J7S1", "uniref50_id": "UniRef50_D1J7S1" }, "pscc": { "uniref50_id": "UniRef50_D1J7S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S1" ] }, "genes": [], "id": "DFOKDJOJIG_386", "locus": "GCA013367855_00351" }, { "type": "cds", "sequence": "contig_1", "start": 190410, "stop": 190880, "strand": "+", "frame": 3, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "COG:COG0782", "COG:K", "GO:0003677", "GO:0032784", "GO:0070063", "RefSeq:WP_012855412.1", "SO:0001217", "UniParc:UPI0001BC20F8", "UniRef:UniRef100_D1J7S2", "UniRef:UniRef50_B3PM73", "UniRef:UniRef90_D1J7S2" ], "nt": "ATGGCTAATAATGATAATAAGATTTATTTAACAGAAGAAAGTTTAAAGCAATATCAAGAAAAATTAAAATATTTACAAGAAGTTGCAAGACCTCAAGTTATTGAAGAAATTAAAGAAGCAAGAAATCAAGGTGACTTGTCAGAAAATGCTGAATATGACTCAGCTCGTGATAAACAAGCTGCTATTGAAAATGAAATTTTAGAAATTCAACATATTCTTGAAAATGTTGAAATTATTAAATCAACATCAACTCATACAATTAGAATTGGTTCAACTGTAAAATTAATAAATATAAACACAAACGAAATAAGCGAAGTTAAAATTGTTGGTGCATTAGATGCCGATCCATTCAACAATAAAATTTCAAATACCTCACCGCTTGCAATTGCATTAAATGGCAAAAAAGTCGGACAAGTGATTGAAGTTGAAGCTCCTAAAAAATATAAAGTTAAAATTGAATCAATTTTATAA", "aa": "MANNDNKIYLTEESLKQYQEKLKYLQEVARPQVIEEIKEARNQGDLSENAEYDSARDKQAAIENEILEIQHILENVEIIKSTSTHTIRIGSTVKLININTNEISEVKIVGALDADPFNNKISNTSPLAIALNGKKVGQVIEVEAPKKYKVKIESIL", "aa_hexdigest": "64b98e0388441a4943fb2bf3806ee693", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20F8", "ncbi_nrp_id": "WP_012855412.1", "uniref100_id": "UniRef100_D1J7S2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20F8", "RefSeq:WP_012855412.1", "UniRef:UniRef100_D1J7S2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S2", "uniref90_id": "UniRef90_D1J7S2" }, "psc": { "uniref90_id": "UniRef90_D1J7S2", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_B3PM73", "cog_id": "COG0782", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM73" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "DFOKDJOJIG_387", "locus": "GCA013367855_00352" }, { "type": "cds", "sequence": "contig_1", "start": 190985, "stop": 191659, "strand": "+", "frame": 2, "gene": "trmN6", "product": "DNA methylase", "db_xrefs": [ "COG:COG4123", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A448ZXG4", "UniRef:UniRef90_D1J7S3" ], "nt": "ATGAAAAATGAAGTTGTTAAAAATAATTTAGGATATAGCGGAGATTTATTTGTATATCAAGATAAATCAATGTTTAATTACTCTGTTGATACTATTTTACTCGGCAATTTTGTATCAATAAACAGAAAAGTAAGAAATATTTTGGAAGTAGGCACGAATAATGGTGCCCTTTCTATTTTTATTGCTGCAAGAGATAAACGTTTGAACATTGATGCTATTGAAATCCAAAGCAAAGCTGTTGAGCTTGCAAGAAAAAATATTATTTTAAACAAACTTGAAAATCAAATTAATCTTATTGAAGGCGATTTTCGGATGTGGGCTAATCAAAAGGCATACGACTGTGGTAATAAATTAGCCAAAAAATACGATTTAATTGTTGCTAATCCGCCTTACTACAATGAAGATTATAATCAACAAAGAAAAAACTCAACTCTTGAACAAAAACTAGCAACTCATGAAATAAATTTAAATTTAGAACAATTAGTTGCTAATAGTGCAAGAATTATTGAACAAAAAGGATACTTAGCAATTGTTTTGCCAATGGCAAGATATATTGATCTAATTTGTTTGCTAAGAAAATACAATTTTGAACCAAAAAGAATTCAATTAGTTTATCCCAGAATCACTGATTTACCAAAATTCTGTTTAGTTGAAGCTAGGTTTAATTCAGGTTGA", "aa": "MKNEVVKNNLGYSGDLFVYQDKSMFNYSVDTILLGNFVSINRKVRNILEVGTNNGALSIFIAARDKRLNIDAIEIQSKAVELARKNIILNKLENQINLIEGDFRMWANQKAYDCGNKLAKKYDLIVANPPYYNEDYNQQRKNSTLEQKLATHEINLNLEQLVANSARIIEQKGYLAIVLPMARYIDLICLLRKYNFEPKRIQLVYPRITDLPKFCLVEARFNSG", "aa_hexdigest": "1bdc4b0dbaa34b36576f4f70f66f78e0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7S3", "query_cov": 1.0, "subject_cov": 0.8615384615384616, "identity": 1.0, "score": 442.0, "evalue": 1.38e-156, "valid": true, "gene": "TrmN6", "product": "DNA methylase", "uniref50_id": "UniRef50_A0A448ZXG4", "cog_id": "COG4123", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4" ], "product": "DNA methylase" }, "genes": [ "trmN6" ], "id": "DFOKDJOJIG_388", "locus": "GCA013367855_00353" }, { "type": "cds", "sequence": "contig_1", "start": 191790, "stop": 192044, "strand": "+", "frame": 3, "gene": null, "product": "Methionyl-tRNA synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X9AHZ7" ], "nt": "ATGAATAATAATAAAAAGACATTTTATATTACCACACCAATTTATTATCCAAGTGGTAATCTTCACATAGGACATTTATTGACTACTACATTAGCTTCAATATATAGAAATTACAAAAATCAACAAGGATATGAAACCTTGTTTATTACTGGAATTGATGAACATGGTCAAAAGATTCAAAAAAAAGCTCTTGAACAAAATATTACTCCAAAAGAATACGTAGATATTCAATCTCAAAAGTTCGTTGATTTATGA", "aa": "MNNNKKTFYITTPIYYPSGNLHIGHLLTTTLASIYRNYKNQQGYETLFITGIDEHGQKIQKKALEQNITPKEYVDIQSQKFVDL", "aa_hexdigest": "3a236a52deb9d8c8ef298f7fa1079aac", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00283F72E3", "query_cov": 0.8809523809523809, "subject_cov": 0.8705882352941177, "identity": 0.608, "score": 94.0, "evalue": 1.58e-23, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A7X9AHZ7" }, "pscc": { "uniref50_id": "UniRef50_A0A7X9AHZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X9AHZ7" ], "product": "Methionyl-tRNA synthetase" }, "genes": [], "id": "DFOKDJOJIG_389", "locus": "GCA013367855_00354" }, { "type": "cds", "sequence": "contig_1", "start": 192129, "stop": 192317, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAAAAGGCTATATTTATAAATCTAAATATGAAGGCTTATATTCAATTAGCGATGAAGAATTCTTAACTAAAACTCAAGCAATATCGAAGGATGGAAAATTTTACCACCCAATTTCAGGACACGAACTTCAAGAAGTAAAAGAAGAAAGTTATTTCTTTAATATGTCAAAATTTACCCCTTGA", "aa": "MEQKGYIYKSKYEGLYSISDEEFLTKTQAISKDGKFYHPISGHELQEVKEESYFFNMSKFTP", "aa_hexdigest": "44f2d0256d7935fe5526ee4f0eb1c69b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7354.149999999999, "isoelectric_point": 5.585135841369628 }, "id": "DFOKDJOJIG_390", "locus": "GCA013367855_00355" }, { "type": "cds", "sequence": "contig_1", "start": 192284, "stop": 192484, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTTGCTTTTCAGCATCTTTAGTTTCTATTTTAATTCCCCAGTCAAAAGAAACTCTTGTTATTGAAAGATCCTCTAATCCTTTATTAATAAAATTATTCATTAATTCTTTATATGTTGCTTTATTGGTAATGAAATCAGGATTTTGTTCGAAATATGACTTTATTCAAGGGGTAAATTTTGACATATTAAAGAAATAA", "aa": "MCCFSASLVSILIPQSKETLVIERSSNPLLIKLFINSLYVALLVMKSGFCSKYDFIQGVNFDILKK", "aa_hexdigest": "9ac09e46bbcc8f05c5f7e880ffa89f5d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7418.862499999998, "isoelectric_point": 8.931272697448733 }, "id": "DFOKDJOJIG_391", "locus": "GCA013367855_00356" }, { "type": "cds", "sequence": "contig_1", "start": 192723, "stop": 193121, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAATCAAAAGGCAATGTTATTGAGCCAATTCCTTTAATTGAAAAATATGGGGCAGAACCCGTTAAGTATTTTTTTGCATCGCAAATAAATATTGACAATGATTTTAGTTTTAGCGAAGAATTACTTATTAATGTATTAAATGCTGATTTAGCAAATAATTTTGGTAATTTAGCCAATAGAACCTTTAAAATGGTAATGTTAAATTTTGAAAATGGTACAACTTATTGTTCAAACCAATTGAATTCATTAGATAAAAATGTTTATTTAGAAATAGAAAATGCATACAATGATTATAAAAAATATTTTGACAATTTTAATGCTGATAAGGCTTTAAAAAGGGCAATTGAATTAAGTAAGTTTTTAAATGAATATATTGATAAAAATGAACCTTGA", "aa": "MSKSKGNVIEPIPLIEKYGAEPVKYFFASQINIDNDFSFSEELLINVLNADLANNFGNLANRTFKMVMLNFENGTTYCSNQLNSLDKNVYLEIENAYNDYKKYFDNFNADKALKRAIELSKFLNEYIDKNEP", "aa_hexdigest": "c6185d837950ffdcf9399d3a9c943b54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15262.991799999985, "isoelectric_point": 4.756821250915528 }, "id": "DFOKDJOJIG_392", "locus": "GCA013367855_00357" }, { "type": "cds", "sequence": "contig_1", "start": 193137, "stop": 193355, "strand": "+", "frame": 3, "gene": null, "product": "Methionyl-tRNA synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PIV2" ], "nt": "TTGCCAAGATTAAATGTGGTTTTAAATACATTGTTGAATGGAATTTATGCAGTTAATATGATGTTAAGCGTTGTTTTAAAAGAAAAATGTAACATTTTATTAAATGATTTGCAATTGTCTGAATTCTCTGAAAAAATGTTGTTTAATTATTCTAAATTTGATAACCAAAAGTTTTCTAAACCTAATATTTTATTTGAAAGGATAAAACCAAATAATTAA", "aa": "MPRLNVVLNTLLNGIYAVNMMLSVVLKEKCNILLNDLQLSEFSEKMLFNYSKFDNQKFSKPNILFERIKPNN", "aa_hexdigest": "da865016a734de266d32dd0609675fbb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PIV2", "query_cov": 0.9444444444444444, "subject_cov": 0.9066666666666666, "identity": 0.5, "score": 64.3, "evalue": 3.96e-12, "valid": false, "product": "Methionyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A3B0PIV2" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PIV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PIV2" ], "product": "Methionyl-tRNA synthetase" }, "genes": [], "id": "DFOKDJOJIG_393", "locus": "GCA013367855_00358" }, { "type": "cds", "sequence": "contig_1", "start": 193376, "stop": 194527, "strand": "+", "frame": 2, "gene": "obgE", "product": "GTPase ObgE", "db_xrefs": [ "COG:COG0536", "COG:DL", "GO:0003924", "SO:0001217", "UniRef:UniRef50_Q4A6N4", "UniRef:UniRef90_D1J7S5" ], "nt": "ATGAAATTTATAGATGAAACAAATGTAATAGTAATAGCTGGCAAAGGTGGCGATGGCATAATTAGTTTCAGAAGAGAAGCTAATGTTGACAAAGGCGGTCCTGATGGTGGCGACGGTGGCGACGGTGGCGATGTTTATTTTGTAGGTGATCCTGGCCAAAATACACTTCTACCATTTTATTATCAAAATAAATTGGTCGCTGAATCAGGAGAAAATGGAAGGCCAAAAAATCAATATGGGGCTGATGGCCAAGATTTAATTGTAAAAGTTCCACTTGGAACAATGGTTTATAATGGCGATGATTTAATAGCCGATGTAGTTAGCCAAGAGAAATACTTAATAGCAAAAGGTGGACACGGTGGCAAAGGAAATCCTAGATTTAAATCTAGTACTAACACTGCTCCTAGAATATGTGAAAATGGTACACCTGGTCAAAAATTTGAACTTCACTTAGTTTTAAAAGTTTTAGCAGATGTTGGCTTTGTTGGAAAGCCTAGTGCTGGCAAGAGCACTATTTTAGGCGAAATATCTAATGCAAAGCCTAAAATTGCAGATTATGATTTTACTACATTGGTTCCTCAACTAGGTTTGGTAAAATATTTTGACAACTCATACGTTGTAGCCGATTTACCAGGACTTATTGAAAATGCTCATTTGGGCAAGGGCCTTGGAATTCAATTTTTAAAGCATATTGAAAGATGTAAAATTATTGCTCATGTTATTGATTTTGGTAGCGAAGAAAAGAATCCAATAGATGATTATGAAATAATTAACAATGAATTAAAATCATATAATTTGTTGCTTGAAAAATTACCACAAGTAGTAATAGCAAACAAAAAAGATCTACCGGCTTTTTCACTAAACTTATCAAAATTTAAAGAAAAATATCCAAATATAAAAGTATTTGAATATTCAGCATTAATGGCTGAAAATTTAGATGAATTAAAAGGCTGCTTGTTTGAAACATTGCAATCTGCTAAAGATTCAATCGCTGTTCAACAAATTCATGAAAGTCAAAACATTGTAAAAATAACATTAAATAAAAAGCCTATTCAAATTAAAAAAATTGATAATGAAACATATGAATTGTTTGGTGATGATATAGATAATATATATAATAAAATACCAGTGATTTCATTAGATAATTTATGA", "aa": "MKFIDETNVIVIAGKGGDGIISFRREANVDKGGPDGGDGGDGGDVYFVGDPGQNTLLPFYYQNKLVAESGENGRPKNQYGADGQDLIVKVPLGTMVYNGDDLIADVVSQEKYLIAKGGHGGKGNPRFKSSTNTAPRICENGTPGQKFELHLVLKVLADVGFVGKPSAGKSTILGEISNAKPKIADYDFTTLVPQLGLVKYFDNSYVVADLPGLIENAHLGKGLGIQFLKHIERCKIIAHVIDFGSEEKNPIDDYEIINNELKSYNLLLEKLPQVVIANKKDLPAFSLNLSKFKEKYPNIKVFEYSALMAENLDELKGCLFETLQSAKDSIAVQQIHESQNIVKITLNKKPIQIKKIDNETYELFGDDIDNIYNKIPVISLDNL", "aa_hexdigest": "e5cd9ea1dbd364c980ce98c147e02431", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7S5", "query_cov": 1.0, "subject_cov": 0.9033018867924528, "identity": 0.995, "score": 740.0, "evalue": 5.77e-269, "valid": true, "gene": "obgE", "product": "GTPase ObgE", "uniref50_id": "UniRef50_Q4A6N4", "cog_id": "COG0536", "cog_category": "DL", "go_ids": [ "GO:0003924" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N4" ], "product": "GTPase Obg" }, "genes": [ "obgE" ], "id": "DFOKDJOJIG_394", "locus": "GCA013367855_00359" }, { "type": "tRNA", "sequence": "contig_1", "start": 194797, "stop": 194873, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 86.0, "nt": "GGGGAAGTAGCTCAGCTTGGTAGAGCACTTGACTTGGGCTCAAGCGGTCGCAGGTTCGAATCCTGTCTTCCTCACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 194814, 194816 ], "id": "DFOKDJOJIG_7", "locus": "GCA013367855_00360" }, { "type": "tRNA", "sequence": "contig_1", "start": 194879, "stop": 194955, "strand": "+", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 78.5, "nt": "GGGGGGGTAGCTCACCTGGCCAGAGCGCCTGCCTTGCACGCAGGAGGTAGAGGGTTCGAGTCCCTTCCTCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 194908, 194910 ], "id": "DFOKDJOJIG_8", "locus": "GCA013367855_00361" }, { "type": "tRNA", "sequence": "contig_1", "start": 194960, "stop": 195036, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 77.9, "nt": "GGCACTGTAGCTCAGTCGGTTAGAGCGTCCGGTTCATACCCGGAAGGTCAAGAGTTCGATTCTCTTCGGTGCTACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 194994, 194996 ], "id": "DFOKDJOJIG_9", "locus": "GCA013367855_00362" }, { "type": "tRNA", "sequence": "contig_1", "start": 195061, "stop": 195136, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 86.1, "nt": "GGACCCATAGCTCAATGGTTAGAGCAACCGGCTCATAACCGGTCGGTTACAGGTTCGAGTCCTGTTGGGTCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "DFOKDJOJIG_10", "locus": "GCA013367855_00363" }, { "type": "tRNA", "sequence": "contig_1", "start": 195139, "stop": 195229, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 74.7, "nt": "GGGCAATTACCCAAGAGGCTGAAGGGAGCAGTCTTGAAAACTGCCAGGGGCTTTACGGCCCGCGGGGGTTCAAATCCCTCATTGCCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 195158, 195160 ], "id": "DFOKDJOJIG_11", "locus": "GCA013367855_00364" }, { "type": "tRNA", "sequence": "contig_1", "start": 195236, "stop": 195311, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 69.0, "nt": "AGCGGAGTAGAGCAGGCGGCAGCTCGTTGGGCTCATAACCCAAAGGTCATTGGTTCAAATCCAATCTCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "DFOKDJOJIG_12", "locus": "GCA013367855_00365" }, { "type": "tRNA", "sequence": "contig_1", "start": 195313, "stop": 195389, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 74.0, "nt": "GGTCCAGTGGTAAAGTGGTTTAATACGCTTCCCTGTCACGGAAGAGATCGCGAGTTCAATTCTCGTCTGGACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 195314, 195316 ], "id": "DFOKDJOJIG_13", "locus": "GCA013367855_00366" }, { "type": "tRNA", "sequence": "contig_1", "start": 195391, "stop": 195466, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 76.9, "nt": "GGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCACTGGTTCGATTCCTGTCGGAGCCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 195424, 195426 ], "id": "DFOKDJOJIG_14", "locus": "GCA013367855_00367" }, { "type": "cds", "sequence": "contig_1", "start": 195554, "stop": 195694, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04193.19" ], "nt": "ATGAATATTAATAGTATAAAAATTGCAATTCAATTATTTGGAATTTTTGGTTCAATTGTAACGATTTGTTTAGGAATTCCACAATTAATTAGATTAAAAAAAACAAAAAACACGGGCAAAGTTAGTTTTGCAGCATTTTGA", "aa": "MNINSIKIAIQLFGIFGSIVTICLGIPQLIRLKKTKNTGKVSFAAF", "aa_hexdigest": "fa5f71c74dafc2ab4e51ccf2c18d046b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04193.19", "name": "PQ-loop", "length": 35, "aa_cov": 0.7608695652173914, "hmm_cov": 1.0, "evalue": 3.738721767842848e-07, "score": 25.503488540649414, "start": 10, "stop": 44 } ], "seq_stats": { "molecular_weight": 5009.071300000001, "isoelectric_point": 10.595137214660646 }, "id": "DFOKDJOJIG_395", "locus": "GCA013367855_00368" }, { "type": "cds", "sequence": "contig_1", "start": 195851, "stop": 196105, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATAAGGGCTATTGTTGGAATAAGCATTAATCAAGTTATTTCATTAATTTTCTTGTGCCTATTTTTTGTTACGGTATATCAAAAGTTGAAATTTGGTAAATTTTATCCCGATAATTCAATTATTCCCGTTCTTCCGGGTAATGCACAACTTGCATTTGGACTAATATGTCCCGCCTTTACAAGTGCAGCATTTTTCCCTGGATTAATTGTTAGTTTAATTAAAAAAGAAGAAATAAGTTTAAGCCCATGA", "aa": "MLIRAIVGISINQVISLIFLCLFFVTVYQKLKFGKFYPDNSIIPVLPGNAQLAFGLICPAFTSAAFFPGLIVSLIKKEEISLSP", "aa_hexdigest": "60c807b458bcf65549d7b3b4131e8543", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9207.040499999996, "isoelectric_point": 9.138925361633301 }, "id": "DFOKDJOJIG_396", "locus": "GCA013367855_00369" }, { "type": "cds", "sequence": "contig_1", "start": 196371, "stop": 196727, "strand": "-", "frame": 2, "gene": "yIH1", "product": "Impact N-terminal domain-containing protein", "db_xrefs": [ "COG:COG1739", "COG:R", "RefSeq:WP_235282753.1", "SO:0001217", "UniParc:UPI000655399F", "UniRef:UniRef100_UPI000655399F", "UniRef:UniRef50_D1J7S7", "UniRef:UniRef90_D1J7S7" ], "nt": "ATGGCAATTTTAGAAATTAAAAAGTCTAAATTTATTTCTTATAAATTTAATATCAAGAGTAAAGATGATGTGAAACTTTTAAAAGAGCAATTAAAAAGTGAACATCAAAAAGCTAGACATATAGTTTATGCTTATTTAGTTATTGAAAATGGCGTTCAATGTGGTGGAATGAGTGATGATGGTGAACCATCAGGAACAGCAGGCAAACCTTTATATTTTTTACTAGAAAGAAATAAATTAAATAATTGTGTGTTGTTTGTAGTTAGATATTTTGGTGGCATAAAATTAGGCGCAAGTAAATTAATTAGAGCATATTCTAAAGTTGCAAATGATGAAATTAAAAGTAGCAATCAGTAA", "aa": "MAILEIKKSKFISYKFNIKSKDDVKLLKEQLKSEHQKARHIVYAYLVIENGVQCGGMSDDGEPSGTAGKPLYFLLERNKLNNCVLFVVRYFGGIKLGASKLIRAYSKVANDEIKSSNQ", "aa_hexdigest": "32d4ee01270a5b1351fa61caf6311b48", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000655399F", "ncbi_nrp_id": "WP_235282753.1", "uniref100_id": "UniRef100_UPI000655399F", "db_xrefs": [ "SO:0001217", "UniParc:UPI000655399F", "RefSeq:WP_235282753.1", "UniRef:UniRef100_UPI000655399F" ] }, "ips": { "uniref100_id": "UniRef100_UPI000655399F", "uniref90_id": "UniRef90_D1J7S7" }, "psc": { "uniref90_id": "UniRef90_D1J7S7", "gene": "YIH1", "product": "Impact N-terminal domain-containing protein", "uniref50_id": "UniRef50_D1J7S7", "cog_id": "COG1739", "cog_category": "R" }, "pscc": { "uniref50_id": "UniRef50_D1J7S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S7" ], "product": "Impact N-terminal domain-containing protein" }, "genes": [ "yIH1" ], "id": "DFOKDJOJIG_397", "locus": "GCA013367855_00370" }, { "type": "cds", "sequence": "contig_1", "start": 196768, "stop": 197661, "strand": "+", "frame": 1, "gene": "rluA", "product": "Pseudouridine synthase", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.23", "KEGG:K06180", "RefSeq:WP_012855418.1", "SO:0001217", "UniParc:UPI0001BC20FE", "UniRef:UniRef100_D1J7S8", "UniRef:UniRef50_Q4A5G9", "UniRef:UniRef90_D1J7S8" ], "nt": "ATGTTAAAAATTATTTCAAATTATTCAGATAGAATAGATAAATATGTTGCTAATAATAGTGAGATTTCAAGAAACGATATTAAGGAATTGATTGAAAATGGCGCAGTATTTGTAAATTCAATAAAGATAAACAAACCTAAATTCGTTGTTAAAGAAGGCGATGAAATTTTAATTGAAAGATTATTAGATAAACAAATAAAGGTTGAACCTCAAAACATTCATTTAGATATAGTGTATGAATGCGATGATTATTTAATAATTAATAAGCCATCAGGCTTAGTTGTTCACCCAGCTCCTGGGCATCATGACAATACATTAGTCAATGCTTTGATGTATCATTTTAAAAATAATTTAAGTGATGTTAATGGTTTATTACGTATGGGGGTTGTTCATAGAATCGATAAAGATACTAGCGGCTTATTATTAATTGCTAAAAACAATAAAGCTCATAACTACTTTGCTTCCCTTTTAAAAACACATGAAATTAAAAGGAGCTATTATGCTATTGTTGAAGGCAAGATTGCAAATGAAGAATTACATATTGAACTTCCAATAGGTAGAGATACTAAAAATCGCCAAAAATATGCTGTTAGTGAAACAAATTCAAAACCCGCTTATACAATAGTAAATGTTGTCAAATATTTTAAAATGAATAATAAAGATTTTTCGTTAGTTAAATGTAATTTAAAAACTGGTCGTACTCACCAAATTAGAGTTCACTTAGCATATATTAAACATCCTGTTTATGGAGATCCAATTTACGGTCACAAAATTGATGATTTCAATCAAAGGCTTCATGCATTTGCTTTAGATTTTGTTGATTTAAATGGAAACAATAAACATTTTGAAGCAGAAATGCCTGAAATTATGAAAAAAGAAATAACTGGCGAATAA", "aa": "MLKIISNYSDRIDKYVANNSEISRNDIKELIENGAVFVNSIKINKPKFVVKEGDEILIERLLDKQIKVEPQNIHLDIVYECDDYLIINKPSGLVVHPAPGHHDNTLVNALMYHFKNNLSDVNGLLRMGVVHRIDKDTSGLLLIAKNNKAHNYFASLLKTHEIKRSYYAIVEGKIANEELHIELPIGRDTKNRQKYAVSETNSKPAYTIVNVVKYFKMNNKDFSLVKCNLKTGRTHQIRVHLAYIKHPVYGDPIYGHKIDDFNQRLHAFALDFVDLNGNNKHFEAEMPEIMKKEITGE", "aa_hexdigest": "c66bfbcb51e14849c3255465f1b3c238", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC20FE", "ncbi_nrp_id": "WP_012855418.1", "uniref100_id": "UniRef100_D1J7S8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20FE", "RefSeq:WP_012855418.1", "UniRef:UniRef100_D1J7S8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S8", "uniref90_id": "UniRef90_D1J7S8" }, "psc": { "uniref90_id": "UniRef90_D1J7S8", "gene": "RluA", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.23" ], "uniref50_id": "UniRef50_Q4A5G9", "cog_id": "COG0564", "cog_category": "J", "kegg_orthology_id": "K06180" }, "pscc": { "uniref50_id": "UniRef50_Q4A5G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5G9" ], "product": "Pseudouridine synthase" }, "genes": [ "rluA" ], "id": "DFOKDJOJIG_398", "locus": "GCA013367855_00371" }, { "type": "cds", "sequence": "contig_1", "start": 197885, "stop": 198034, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAATATGCTGAACTTTTTGGCCAAATCCAAAAATTAAATCCAAATTTTCAAGGAGATGTATTCGCACTAGCCAATAATGAATATGTCAAATCTTTGGCAATGTCAGGCGTTTTACTTGTAATTTTAATTGCGGTTTATGTTTGA", "aa": "MNKYAELFGQIQKLNPNFQGDVFALANNEYVKSLAMSGVLLVILIAVYV", "aa_hexdigest": "448b2300fae38c03cd81a8f528029e4b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5448.3564, "isoelectric_point": 5.936628150939941 }, "id": "DFOKDJOJIG_399", "locus": "GCA013367855_00372" }, { "type": "cds", "sequence": "contig_1", "start": 198206, "stop": 198685, "strand": "+", "frame": 2, "gene": null, "product": "Rho termination factor N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S9", "UniRef:UniRef90_D1J7S9" ], "nt": "GTGGCAACATTGTTATATATAGTTTCATATTTTGCTGTATTTTTAAAATGCTCTTATGTCGCAAAACTATTTTTAATTTTAAAGAGAAATTTAGCATTTAAGGCATTAAACAACTCGGGCTCAATGGATGATATTTTAAGATCATATTTCTATGGTCAAGAATCTCCTGAACCAACACTAAATACCAATAATACATTTGATTCAAATCAAAAAACCGAAGAAAACAAATCTGAAGATTTAAACAAAGAAGAAGATTCAAAATCAATTGAATATCGTAGTAAATTAGAACAATTAAATGATGAACAATTGCAAAATATGGCTAAAAAATTAAATATTTTTGGAATTGAAACATTTTCAAGAGAACAATTGATTTCAAAAATTAGCGATATTTTTGAACAATCAGCCAAGTCTCAAAATCAACAATCAAAACCAGATAATTCCGATGAAGACAACAACGGCGGAAGTCAAACCAGCAATTAA", "aa": "MATLLYIVSYFAVFLKCSYVAKLFLILKRNLAFKALNNSGSMDDILRSYFYGQESPEPTLNTNNTFDSNQKTEENKSEDLNKEEDSKSIEYRSKLEQLNDEQLQNMAKKLNIFGIETFSREQLISKISDIFEQSAKSQNQQSKPDNSDEDNNGGSQTSN", "aa_hexdigest": "a35b946c0b80ced682a5dae03d9114c4", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 197906, "stop": 198685, "observations": { "mutation": [ 198032, 198179 ], "stop": [ 198032, 198179 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7S9", "query_cov": 0.9937106918238994, "subject_cov": 0.49221183800623053, "identity": 0.975, "score": 239.0, "evalue": 1.57e-76, "gene_start": 2, "gene_end": 159, "reference_start": 164, "reference_end": 321, "reference_sequence": "MKSININEYNSTNELYTKNKKVFWAWFIAWSVLFATILIFVIASFIQFAVEKQNYLNKYAELFGQIQKLNPNFQGDVFALANNEYVKSLAMSGVLLVILIAVYVWHIVSIALVAKNKDFSAYSRILTTLYLFVIIYEIITLIFSGFQSFSLAIWQVNKILNLVATLLYIVSYFAVFLKCSYVAKLFLILKRNLAFKALNNSGSMDDILRSYFYGQESPESTLNTNNTFDSNQKTEENKSEDLNKEEDSKSIEYRSKLEQLNDEQLQNMAKKLNIFGIETFSREQLISKISDIFEQSAKSQNQQSKPDNSDEDNNDGSQTSN" }, "paralog": false, "identity": 0.9768339768339769, "score": 441.0, "evalue": 1.44e-158, "description": "Internal stop codon at 198032, 198179. Nonsense mutation around 198032, 198179.", "psc": { "uniref90_id": "UniRef90_D1J7S9", "product": "Rho termination factor N-terminal domain-containing protein", "uniref50_id": "UniRef50_D1J7S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7S9", "UniRef:UniRef50_D1J7S9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S9" ], "product": "Rho termination factor N-terminal domain-containing protein" } }, "id": "DFOKDJOJIG_400", "locus": "GCA013367855_00373" }, { "type": "cds", "sequence": "contig_1", "start": 198727, "stop": 198822, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATCAAACATCATTAAAACAACATATTTTTACTTTTTTTTCACAAAATCCTCCTGCTAGTTCAATAAAAAATGGTGATCAAAAATTATGA", "aa": "MNNQTSLKQHIFTFFSQNPPASSIKNGDQKL", "aa_hexdigest": "1850d34680cd35cdae2d863bb2a7a0fd", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3521.910500000001, "isoelectric_point": 9.700380516052245 }, "id": "DFOKDJOJIG_401", "locus": "GCA013367855_00374" }, { "type": "cds", "sequence": "contig_1", "start": 199111, "stop": 199566, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGCGCTTTCAATTGTTTTAATAATATCAATTAATTTATCACTATTAGCAATTTATTTTGTTTCATTTAAAGAAGCTAAGTTTGTTAATAAAAAAAATTTAGAAGATGAGCTTATTTCTTTGAAGTATAAAAAATCCAAAGAATTAAATGATTTAAATGCCCCTGAAAATATTAAACTGGATATCAAAGCAATGGAATCACACAACCCATTAGCCCCTAGAGTGTTAGCAAAATTTATTGATTTTTATAACAAAATGTCAAGTATTGAATTATTTAAGCGTTATAAGAATTATTTAAATCAAACATTCAAAATTAGATATTTTAATGAATCTCTTGAAGTAATTGAAGGAAAAAATGAAATGGAAGATAATCTTGAAAAATATCTAGACAACATAAATAATAAAGATCCTGAATCACAAAAAAATTCTCTAATTTTAAAAGAAATTGCTTGA", "aa": "MIALSIVLIISINLSLLAIYFVSFKEAKFVNKKNLEDELISLKYKKSKELNDLNAPENIKLDIKAMESHNPLAPRVLAKFIDFYNKMSSIELFKRYKNYLNQTFKIRYFNESLEVIEGKNEMEDNLEKYLDNINNKDPESQKNSLILKEIA", "aa_hexdigest": "f17dfa072a591aad412b37b5ea230221", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17699.3643, "isoelectric_point": 7.786249732971191 }, "id": "DFOKDJOJIG_402", "locus": "GCA013367855_00375" }, { "type": "cds", "sequence": "contig_1", "start": 199567, "stop": 199719, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATATGGATAAAAAGGTTAAAATTATTAGAGAAGCAGATAAAAATATAACCATGTCTAAAAAAGAATTTCAAGAAAAATATGATGAATATGTTTATTTGCAAAGATCACAAGATCCTGCATATCAACAAACTCAAAAGAATACATGA", "aa": "MNDMDKKVKIIREADKNITMSKKEFQEKYDEYVYLQRSQDPAYQQTQKNT", "aa_hexdigest": "01d8f0b425674717c14c6c03c8d4da74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6119.825400000004, "isoelectric_point": 7.9425855636596685 }, "id": "DFOKDJOJIG_403", "locus": "GCA013367855_00376" }, { "type": "cds", "sequence": "contig_1", "start": 199912, "stop": 200511, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_175972232.1", "SO:0001217", "UniParc:UPI00158EA8B6", "UniRef:UniRef100_UPI00158EA8B6", "UniRef:UniRef50_D1J7T1", "UniRef:UniRef90_D1J7T1" ], "nt": "ATGACTAAGAAACACAAAAGAATTTTATTTTTATCAACATTAGCATTACCCGTAGCCCTTGTGCCAGCAACTATGGTTTCATGTTCAAAAAAAGATATTGAAGAAATAAAATTGGATATGATCACAATAGCAAAAAATCAAATGCTAAATTATTTACAAAAATTTGAATCATTTTTTAAAAATGATGATGATCCTGATGTTAGAAATTTAGAAGTAGAATTTAATAAAATTATAATTTCATTAAAAAAAATGGATATAACACCTGATTTTAATATGGATCAATTTATTCAAGAAATGACTAAAAAATTTAGCGCTGTAGCAAATAATTTTTTGAATATAAAATACAATAAGGCCAAATTAACAAGTGAATATTATATTTCAAAAGTAAAAGCTCAGCTAATTTTGGAGCAATTTAAAAATTCTGGTATTCTTCCAGAAAAAAATGAATTTGCCAAGATTTTAGAGCAAGAATTATCAAAAGTTTCCGATCCGACTATTAAAACCTCTGAAGAAGAGTTAAAAAAATCAATTAGATTTTTAGATGATTTAAACAAAAAATATAATGAAATTTTAAAAAAGCAAGTAATGATTTCAAAATAA", "aa": "MTKKHKRILFLSTLALPVALVPATMVSCSKKDIEEIKLDMITIAKNQMLNYLQKFESFFKNDDDPDVRNLEVEFNKIIISLKKMDITPDFNMDQFIQEMTKKFSAVANNFLNIKYNKAKLTSEYYISKVKAQLILEQFKNSGILPEKNEFAKILEQELSKVSDPTIKTSEEELKKSIRFLDDLNKKYNEILKKQVMISK", "aa_hexdigest": "ce9040227e81df3c43411be5de32498c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158EA8B6", "ncbi_nrp_id": "WP_175972232.1", "uniref100_id": "UniRef100_UPI00158EA8B6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EA8B6", "RefSeq:WP_175972232.1", "UniRef:UniRef100_UPI00158EA8B6" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158EA8B6", "uniref90_id": "UniRef90_D1J7T1" }, "psc": { "uniref90_id": "UniRef90_D1J7T1", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J7T1" }, "pscc": { "uniref50_id": "UniRef50_D1J7T1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T1" ], "product": "Lipoprotein" }, "genes": [], "id": "DFOKDJOJIG_404", "locus": "GCA013367855_00377" }, { "type": "cds", "sequence": "contig_1", "start": 200674, "stop": 201090, "strand": "+", "frame": 1, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02950", "RefSeq:WP_175972233.1", "SO:0001217", "UniParc:UPI0015897B0A", "UniRef:UniRef100_UPI0015897B0A", "UniRef:UniRef50_P92532", "UniRef:UniRef90_A0A858U5X2" ], "nt": "ATGCCTACAGTTGCACAATTAGTAAAACAAGGCCGTAAAGATAAAACAACAAAATCTAAAGCTCCTGCTTTAGGAAAAATGTATAACTCTTTACAAAAAAAAGAAACTTCTATGCCCGCACCATTTAAGCGTGGCGTATGTACTCGTGTGGCAACAATGACACCTAAGAAACCTAACTCAGCTATTAGAAAATATGCCCGTGTTAGATTATCAAATGGTCAAGAAGTTACTGCCTATATTCCCGGTGAAGGACACAATTTACAAGAACACAGTGTTGTTTTAATTCGTGGAGGTAAGGTTAAAGATTTACCCGGAGTTAGATACACAATTGTTCGTGGAACACAAGATGCTGCCGGAGTTAATAACAGAAAGCAAGCTCGTTCTATTTATGGAACTAAAAAACCAAAAAGTAATTAA", "aa": "MPTVAQLVKQGRKDKTTKSKAPALGKMYNSLQKKETSMPAPFKRGVCTRVATMTPKKPNSAIRKYARVRLSNGQEVTAYIPGEGHNLQEHSVVLIRGGKVKDLPGVRYTIVRGTQDAAGVNNRKQARSIYGTKKPKSN", "aa_hexdigest": "02783ea2c43e3dc6f2ba29ea7c7acaaf", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0015897B0A", "ncbi_nrp_id": "WP_175972233.1", "uniref100_id": "UniRef100_UPI0015897B0A", "db_xrefs": [ "SO:0001217", "UniParc:UPI0015897B0A", "RefSeq:WP_175972233.1", "UniRef:UniRef100_UPI0015897B0A" ] }, "ips": { "uniref100_id": "UniRef100_UPI0015897B0A", "uniref90_id": "UniRef90_A0A858U5X2" }, "psc": { "uniref90_id": "UniRef90_A0A858U5X2", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "genes": [ "rpsL" ], "id": "DFOKDJOJIG_405", "locus": "GCA013367855_00378" }, { "type": "cds", "sequence": "contig_1", "start": 201121, "stop": 201429, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "nt": "ATGTCAAGAAAACATAAAGCGCCTGTTAGAGATGTCTTAGCAGATCCAGTTTTTAACTCTAAGATAATTACTAAATTAGTAAATACCATTATGTTAGATGGTAAAAAATCTATTGCTGAAAACATTTTATACAGTGCATTTGATATTATTAAAACAAAAACAGGAAAAGAACCAGTTGAAGTATTTAATCTAGCATTAGAAAACGTTAGTCCACAACTAGAAGTTAGATCAAGAAGAGTTGGTGGTTCAAACTACCAAGTTCCTTGTGAAGTTTCTGCAAAAAGAAAACAAACTTTAGCATTAAGATGA", "aa": "MSRKHKAPVRDVLADPVFNSKIITKLVNTIMLDGKKSIAENILYSAFDIIKTKTGKEPVEVFNLALENVSPQLEVRSRRVGGSNYQVPCEVSAKRKQTLALR", "aa_hexdigest": "a846a895e05ab362d597ef3c44d7d680", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A381KMK1", "query_cov": 1.0, "subject_cov": 0.8429752066115702, "identity": 0.5589999999999999, "score": 124.0, "evalue": 1.23e-34, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A381KMK1", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A381KMK1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "product": "Ribosomal protein S7" }, "genes": [], "id": "DFOKDJOJIG_406", "locus": "GCA013367855_00379" }, { "type": "cds", "sequence": "contig_1", "start": 201466, "stop": 201588, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948V4F4", "UniRef:UniRef90_J9ZBC4" ], "nt": "ATGGAAGAAAAGTTAGCAAATGAATTAATAGATGCCTCAAACAAAGTTGGTGGCGCTATTAAGAAACGTGAAGATACCCATAAAATGGCTGAATCTAACAAAGCATTTGCTCACTTTAGATGA", "aa": "MEEKLANELIDASNKVGGAIKKREDTHKMAESNKAFAHFR", "aa_hexdigest": "c45ebf744fe7d637b0f9acfff3932c27", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.775, "score": 69.7, "evalue": 4.19e-15, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 201415, "stop": 201588, "observations": { "mutation": [ 201427 ], "stop": [ 201427 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_J9ZBC4", "query_cov": 1.0, "subject_cov": 0.6896551724137931, "identity": 0.8, "score": 69.3, "evalue": 7.18e-15, "gene_start": 1, "gene_end": 40, "reference_start": 18, "reference_end": 57, "reference_sequence": "MALRWIVDSAYKRAGHSMEEKLAGELLDASNNTGGAVKKREDTHRMAEANKAFAHYRW" }, "paralog": false, "identity": 0.6666666666666666, "score": 79.0, "evalue": 1.24e-21, "description": "Internal stop codon at 201427. Nonsense mutation around 201427.", "psc": { "uniref90_id": "UniRef90_J9ZBC4", "product": "Ribosomal protein S7", "uniref50_id": "UniRef50_A0A948V4F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_J9ZBC4", "UniRef:UniRef50_A0A948V4F4" ] } }, "id": "DFOKDJOJIG_407", "locus": "GCA013367855_00380" }, { "type": "cds", "sequence": "contig_1", "start": 201604, "stop": 201759, "strand": "+", "frame": 1, "gene": "lepA", "product": "GTP-binding protein lepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4", "UniRef:UniRef90_A0A3P8KNQ4" ], "nt": "ATGTCTCGTGAATATAAATTAGAAGATTACCGCAATATCGGAATCATGGCTCACATTGATGCCGGAAAAACCACAACTACTGAACGTGTGTTATATCACACAGGAAAAATTCATAAAATTGGTGAAACACACGAAGGTGCTTCACAAATGGACTGA", "aa": "MSREYKLEDYRNIGIMAHIDAGKTTTTERVLYHTGKIHKIGETHEGASQMD", "aa_hexdigest": "819aa66155ed42a00a7c7201fbcb4d6f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003516DBAA", "query_cov": 1.0, "subject_cov": 0.9622641509433962, "identity": 0.725, "score": 84.7, "evalue": 8.03e-21, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A962BSW7" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 201604, "stop": 201816, "observations": { "mutation": [ 201757 ], "stop": [ 201757 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "query_cov": 1.0, "subject_cov": 0.6891891891891891, "identity": 0.882, "score": 100.0, "evalue": 5.36e-27, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MARQYKLEDYRNIGIMAHIDAGKTTTTERILLHTGKIHKLGETHDGASQMDWMAQEQERGITITSAATNSILKW" }, "paralog": false, "identity": 0.8571428571428571, "score": 126.0, "evalue": 8.44e-40, "description": "Internal stop codon at 201757. Nonsense mutation around 201757.", "psc": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KNQ4", "UniRef:UniRef50_A0A3P8KNQ4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "product": "GTP-binding protein lepA" } }, "id": "DFOKDJOJIG_408", "locus": "GCA013367855_00381" }, { "type": "cds", "sequence": "contig_1", "start": 202012, "stop": 203127, "strand": "+", "frame": 1, "gene": null, "product": "GTP dependent elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A346FYQ4" ], "nt": "ATGGACAAAATGGGTGCTAACTTTAAAGCATCTGTTGAATCACTTCATAAATTACTTGGTGCTAATGCTCACGCTATTCAATTAAATATTGGTGAAGAAAACCATTTTGTTGGAATCGTTGATTTAGTTGAAATGAAAGCATATTCATATGATGGCGAAGTTGATGAAAACGCTAAAGAAATTGCTATTCCAGAACATTTAAAAGATGAAGCTCATATTATGAGAGCTGAATTAGCTGAATCACTTGTTGATTATGATGAAGAACTTATGACTTTATTATTAGAAGAAAAAGAAGTTCCAGTTGACATTTTAAAAAGAGCAATTAGAACTGCTACAATTTCTTCTGAATATTTCCCAGTTGTTTGTGGAACATCATTTAAAAACAAAGGTGTTAAGTTAATGCTTGATGCAATTGTTGATTATTTACCAAGTCCTTTAGATATTCCTCCAATGAAGGCATATAAAGGCGAATCAGAAATTCATATCCCTGCCTCAGATGACGAATTTTTCTCAGCTTTAGCATTCAAAGTTATGAATGACCCATTTGTTGGAAACCTAACATACTTTAGAGTTTACTCAGGAATTGTTTCAAAAGGTTCTTATGTATTCAATTCAACAAAAGGTGAAAAAGAAAGACTAAGTAGAATTCTATTGATGCATGCTAATAGTCGTACAGACATTGACGAAGTTAGAACAGGAGATATTGCTGCAGCAGTTGGTTTGAAAGTAACAACCACTGGAGATACATTGATTGATGAAAAACAAAAAGATGTTGTATTGGAAAATATGAATTTCCCTGAACCAGTTATATCTCAAGCTATTGAACCAAAGACCAAAGATGCTTCTGAAAAATTATCATTAGCATTGCAAAGATTGGCTGCTGAAGACCCAACATTTAAATACTACACCGATGAAGAAACAGGACAAACAATTATTGCAGGTATGGGTGAACTTCACCTTGACATTATTGTTGACCGTCTAAGACGTGAATTTAAAGTTGAAGTAACAGTTGGCGCACCACAAGTTTCATATCGTGAAACTATTACAAAGACTGCCGATGTTGAAGGTATGCACAAAAAGCAATCTGGTGGTAAAGGTCAATATGGACACGTTTGA", "aa": "MDKMGANFKASVESLHKLLGANAHAIQLNIGEENHFVGIVDLVEMKAYSYDGEVDENAKEIAIPEHLKDEAHIMRAELAESLVDYDEELMTLLLEEKEVPVDILKRAIRTATISSEYFPVVCGTSFKNKGVKLMLDAIVDYLPSPLDIPPMKAYKGESEIHIPASDDEFFSALAFKVMNDPFVGNLTYFRVYSGIVSKGSYVFNSTKGEKERLSRILLMHANSRTDIDEVRTGDIAAAVGLKVTTTGDTLIDEKQKDVVLENMNFPEPVISQAIEPKTKDASEKLSLALQRLAAEDPTFKYYTDEETGQTIIAGMGELHLDIIVDRLRREFKVEVTVGAPQVSYRETITKTADVEGMHKKQSGGKGQYGHV", "aa_hexdigest": "88759284aeea12371ea334a637aa3f21", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000DB1047A", "query_cov": 0.9649595687331537, "subject_cov": 0.8306264501160093, "identity": 0.534, "score": 344.0, "evalue": 7.19e-113, "valid": false, "product": "translation factor GTPase family protein", "uniref50_id": "UniRef50_A0A346FYQ4", "kegg_orthology_id": "K02355" }, "pscc": { "uniref50_id": "UniRef50_A0A346FYQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A346FYQ4" ], "product": "GTP dependent elongation factor G" }, "genes": [], "id": "DFOKDJOJIG_409", "locus": "GCA013367855_00382" }, { "type": "cds", "sequence": "contig_1", "start": 203239, "stop": 203610, "strand": "+", "frame": 1, "gene": null, "product": "Elongation factor G domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AGV8" ], "nt": "ATGGCTATTGGTATTTTAGCAGGATACCCTATGATAGATATCAAAGCTACTTTATTTGATGGATCATACCACGAAGTTGACTCGTCTGAATTAGCATATAAAATTGCTGCTTCTAAATCACTTACAAAAGGCCGTGAAGCCTTGGGAACAGTATTACTAGAACCTATTATGGATGTTGCTGTTGTTGTTCCTGAAGACTTCTTTGGAGATGTTATGGGTGATATTTCTAGAAGAAGAGGACAAGTTAGAGATAACGAAACAAGAAACGATGGAGCCCATGTTATTAAAGCATATATTCCATTGAGTGAAATGTTTGGTTATGCAACTCAATTACGTTCAATGACTACAGGTCGTGGAACATACCAAATGTGA", "aa": "MAIGILAGYPMIDIKATLFDGSYHEVDSSELAYKIAASKSLTKGREALGTVLLEPIMDVAVVVPEDFFGDVMGDISRRRGQVRDNETRNDGAHVIKAYIPLSEMFGYATQLRSMTTGRGTYQM", "aa_hexdigest": "96f465eb36f63e8aba1a1d4f44085104", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449AGV8", "query_cov": 1.0, "subject_cov": 0.9318181818181818, "identity": 0.772, "score": 194.0, "evalue": 3.83e-62, "valid": false, "product": "Elongation factor G domain protein", "uniref50_id": "UniRef50_A0A449AGV8" }, "pscc": { "uniref50_id": "UniRef50_A0A449AGV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AGV8" ], "product": "Elongation factor G domain protein" }, "genes": [], "id": "DFOKDJOJIG_410", "locus": "GCA013367855_00383" }, { "type": "cds", "sequence": "contig_1", "start": 203956, "stop": 204171, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTCAAGAAACTCGAGATAAATTAATCTTAGCCAACGCCTTGCCTTCTGTTTCGGGTGCAATGTTACAAGTTAGATTTACATTTACCTATATTAGTAACATTTTAATAGGTTTTGCATTGATATTTTATGGTTTACTTCCTAATTATCTATGGACAAAAAGAGGATTTTTTTTAGCAACTGTTTATATAAATATAACATTCTTTACTTTTTGA", "aa": "MSQETRDKLILANALPSVSGAMLQVRFTFTYISNILIGFALIFYGLLPNYLWTKRGFFLATVYINITFFTF", "aa_hexdigest": "53e70e2870cc45461ed2b56fb7457907", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8180.5798999999915, "isoelectric_point": 9.694836235046388 }, "id": "DFOKDJOJIG_411", "locus": "GCA013367855_00384" }, { "type": "cds", "sequence": "contig_1", "start": 204397, "stop": 204720, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGTAACTAATTTATTTTTAATTGAACAATTTAATAAAAAAACAACTGGGCTTGATATTTTTACAAAAACTAAGGTGGCAATCTATCCCTTTTTAAATTTAAAATATCCTCTTGGTTATCATGGCAATAATTTGTTTTATAAAATTCTATTAAATTTATTTATCCCAATATTTGGAATTATTGTATGTATATCTGTTGCTTATTTTTTAAAATTTATTTGTAAGATTCAAACGTATTCAAAAAAAGATTTTGAAAATCCTGCGTTTTTAAGAGAAAATAAGCTCTTAATAAAATCAATAAAATCACAAATTTTTAAGTAA", "aa": "MQVTNLFLIEQFNKKTTGLDIFTKTKVAIYPFLNLKYPLGYHGNNLFYKILLNLFIPIFGIIVCISVAYFLKFICKIQTYSKKDFENPAFLRENKLLIKSIKSQIFK", "aa_hexdigest": "4d283377d9f4a9083156c97c15d29ca5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12580.982599999981, "isoelectric_point": 9.758853340148924 }, "id": "DFOKDJOJIG_412", "locus": "GCA013367855_00385" }, { "type": "cds", "sequence": "contig_1", "start": 204732, "stop": 205361, "strand": "+", "frame": 3, "gene": null, "product": "DUF1846 domain-containing protein", "db_xrefs": [ "COG:COG4868", "COG:S", "SO:0001217", "UniRef:UniRef50_D1J7T6", "UniRef:UniRef90_D1J7T6" ], "nt": "ATGACGGCTTTTAATTTAAAAAAATATTTTGAGTTACAAACAATTGAAATTAAAAATAGAATTAAGCAATTTAACAATAAACTATATTTAGAATTTGGTGGCAAAATTTTTGACGATTTACATGCTTCAAGGGTTTTACCCGGCTTTTTAAGCGATAGCAAATTACAATTATTGTTAAAATTAAAAAACGAAGCAGAAATTGTAGTTGTAATTTCTGCTAATGATATTGAAAACAATAAATTGCGTGGTGATCTAGGAATCAAATATGAAGACGATGTATTGAGACTAATTGATAACTTTAAAAGTTACGATTTAATTGTAAATAATGTAGTTATTACCCAATATCAACCAACATTTAGAGTTAATAAACTGCAAAACATTCTTAGTGAACATAATATCAATGTCGTTAAACACTATAAGATTGACGATTATCCTAATAATTTAGATTTAATAGTTTCAAAAAATGGATATGGCAAAAATGAAACTATAAAAACTCAAAAGCCATTAATAGTAATTACTGCCCCTGGACCAGGTTCTGGAAAATTAGCAGTTGCATTAAGCGAACTTTATCATGATGCAATAAATAATATAGATTCTGGTTATGCAAAATTTGAAACTTTTCCCGTTTGA", "aa": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQPTFRVNKLQNILSEHNINVVKHYKIDDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPV", "aa_hexdigest": "7ec6c64c5c6133c238e47fec504b43df", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0025EA5487", "query_cov": 0.9904306220095693, "subject_cov": 0.8922413793103449, "identity": 0.57, "score": 244.0, "evalue": 7e-79, "valid": false, "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_A0A348P2W6" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 204732, "stop": 205664, "observations": { "mutation": [ 205359 ], "stop": [ 205359 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7T6", "query_cov": 1.0, "subject_cov": 0.4247967479674797, "identity": 0.99, "score": 408.0, "evalue": 7.63e-140, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQSTFRVNKLQNILSEHNINVVKHYKIEDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPVWNLAVDHPLNLAYEAATTDLNDQNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINIIGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK" }, "paralog": false, "identity": 0.9870967741935484, "score": 608.0, "evalue": 4.59e-221, "description": "Internal stop codon at 205359. Nonsense mutation around 205359.", "psc": { "uniref90_id": "UniRef90_D1J7T6", "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_D1J7T6", "cog_id": "COG4868", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7T6", "UniRef:UniRef50_D1J7T6", "COG:COG4868", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T6" ], "product": "DUF1846 domain-containing protein" } }, "id": "DFOKDJOJIG_413", "locus": "GCA013367855_00386" }, { "type": "cds", "sequence": "contig_1", "start": 205383, "stop": 206210, "strand": "+", "frame": 3, "gene": null, "product": "DUF1846 domain-containing protein", "db_xrefs": [ "COG:COG4868", "COG:S", "SO:0001217", "UniRef:UniRef50_D1J7T6", "UniRef:UniRef90_D1J7T6" ], "nt": "TTGAATTTAGCTTATGAAGCTGCAACAACAGATTTAAATGATCAAAACAAAATCGATCCTTATTATTTAAAAAGTCGCAATAAAATAGCAACAAATTATAACCGTGATATTCAAGCATTTCCAATTTTAAGTCAAATAATGAACAAAATTCAAAAACGTAATATTTATGATTCACCAACATCAATGGGAGTTAATATGGTTGGATTTTGTTTTAATAATGAAGAGGAAATTAAACAAGCAGCAAATAAAGAAATAATCCGTCGCTACTATCAAAGTGCTGTTGCTTTTAAAAAAGGTATGGTTTCTAAATCAGTATTAATAAGAAATGCAAACTTGATATCTAAAAATAATATTGATTTTTCAATTAGACCTTGCATTGAAATTGCTAACAAATTAGCAAATCAATTGAATATGAATATCACAACCTTTACCTTAAACAACAATATAAATATTATAGGTAAGACATCAGCGCTTCTTGGCTCATCATCAGCTGCGTTATTAAATGCATTAAAATATTTAGCAAATATTCCTAAAGAAATCCATTTACTATCAAAAGAAGCAATTGAATTAGTTCAAAATCTTAAAGTAAATGTTTTTAAAAGCAAAAATCCCCGCTTACATATGAATGAAACTTTAATAATTTTAGCAATGTCTGCTAAGAGCAACCAATATGCAAAATTAGCCTATGATCAATTAAAAAATTTAGCAGGTATCGAAGCACATTGCAGTGCAATACTTTCTATGCAAGATATTGAAACATACAATCATTTAAATATTAACATTACCCAAGAACCAAAATACGATAATGACACCCTATATCATAAATAG", "aa": "MNLAYEAATTDLNDQNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEEEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINIIGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK", "aa_hexdigest": "3062a9865ac52da4fea746a2fce5415a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 205083, "stop": 206210, "observations": { "mutation": [ 205359 ], "stop": [ 205359 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7T6", "query_cov": 1.0, "subject_cov": 0.5589430894308943, "identity": 0.993, "score": 528.0, "evalue": 6.58e-186, "gene_start": 1, "gene_end": 275, "reference_start": 218, "reference_end": 492, "reference_sequence": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQSTFRVNKLQNILSEHNINVVKHYKIEDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPVWNLAVDHPLNLAYEAATTDLNDQNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINIIGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK" }, "paralog": false, "identity": 0.992, "score": 735.0, "evalue": 7.73e-270, "description": "Internal stop codon at 205359. Nonsense mutation around 205359.", "psc": { "uniref90_id": "UniRef90_D1J7T6", "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_D1J7T6", "cog_id": "COG4868", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7T6", "UniRef:UniRef50_D1J7T6", "COG:COG4868", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T6" ], "product": "DUF1846 domain-containing protein" } }, "id": "DFOKDJOJIG_414", "locus": "GCA013367855_00387" }, { "type": "cds", "sequence": "contig_1", "start": 206264, "stop": 206827, "strand": "-", "frame": 3, "gene": "comEB", "product": "Deoxycytidylate deaminase", "db_xrefs": [ "COG:COG2131", "COG:FJ", "SO:0001217", "UniRef:UniRef50_B3PML8", "UniRef:UniRef90_D1J7T7" ], "nt": "ATGTTAAAATATTTTATTATGAATAATAAAACAAGAAAATTAATTTTTAACAACCATAAAGATAATTTAAAATGGGATATTTATTTTATGTCACTTGCCAAATTAAGTGCATTACGTTCAAAAGATCCTTTAACTAAGGTGGGAGCTTGTATTGTAAGTCCAGATAATTATGTAATATCGCTAGGATATAATGGTATGCCAACAAGTTATTTAAATAAAGAAGTTAACAATGATGATTTATTCACTTGGAATAGACCTGCGACCCAAAACGATGTCTTAAATTCCAAATATACTTACGTTGTCCATGCCGAAGCAAATGCAATAATAAATGCAAATATTACAAATTCAAAGATTATACCAGGGTCGATATTATACGTAACACATTCACCATGCTACCACTGTGCTAAGCTAATTGTCCAATCTAAGATTTCTAAAGTTGTTTATGCAGTTGCATATAAGCCTGATTCAGATGATTTTAAAGCTTCAAATAAAATTTTTGCTGCCTTCAATATTGAATGCAAAGCAATAGAAAACGATTTTGATTTAGATTTTCATATAAAATAA", "aa": "MLKYFIMNNKTRKLIFNNHKDNLKWDIYFMSLAKLSALRSKDPLTKVGACIVSPDNYVISLGYNGMPTSYLNKEVNNDDLFTWNRPATQNDVLNSKYTYVVHAEANAIINANITNSKIIPGSILYVTHSPCYHCAKLIVQSKISKVVYAVAYKPDSDDFKASNKIFAAFNIECKAIENDFDLDFHIK", "aa_hexdigest": "516ddf32e8166ced4250f49384e07404", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7T7", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9890000000000001, "score": 379.0, "evalue": 4.5e-133, "valid": true, "gene": "ComEB", "product": "Deoxycytidylate deaminase", "uniref50_id": "UniRef50_B3PML8", "cog_id": "COG2131", "cog_category": "FJ" }, "pscc": { "uniref50_id": "UniRef50_B3PML8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PML8" ], "product": "Deoxycytidylate deaminase" }, "genes": [ "comEB" ], "id": "DFOKDJOJIG_415", "locus": "GCA013367855_00388" }, { "type": "cds", "sequence": "contig_1", "start": 207888, "stop": 208304, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGCAACATATACAGGTAGTGAACTTGTTAGAAAACAAGCCTTAACTCAAACAAAAACTATAACTATAACAGAAAAAGAAACTAAAAAAACCACTACCTATTTAGTAAAACCTACTTTTGCTAAATATAGATTAGAATTAGCAGAAGCTATTGTTTTAACTGAAAAAGAAACTAATAAACAATATGTATTTGATTCAGATGAAATTCCAAGTGAATCTGAATATCCACAACCAACTAAAACTGTAACAGATATTAATGGCAAAGAATACAAAGTTTATGAAAAAACAAACGTTTTTGTTAAAACAAAAGGTGACAAGAACGTTGAATCAAGATCAATTAATAGTGCATTCTTTTTAGACAAATTAGCTAATGCCTCAAAATTACAATTTGTAATTAGAAAAGGTGTTAAATGA", "aa": "MPATYTGSELVRKQALTQTKTITITEKETKKTTTYLVKPTFAKYRLELAEAIVLTEKETNKQYVFDSDEIPSESEYPQPTKTVTDINGKEYKVYEKTNVFVKTKGDKNVESRSINSAFFLDKLANASKLQFVIRKGVK", "aa_hexdigest": "caa3cb110fb8e2e5196f7ec20b96a9b3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15742.795399999986, "isoelectric_point": 9.356054878234861 }, "id": "DFOKDJOJIG_416", "locus": "GCA013367855_00389" }, { "type": "cds", "sequence": "contig_1", "start": 208515, "stop": 208859, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCGGCATTGATGCTAATAAATTATTTGAAAAAGATAAATTTATTCAAAAAGTATCAGCTGAAGGTAATTTAAAAGATAGAGAAGCAATTACTTTTGAATCAATTAATGGAAAAGAAAAAGATGCTAACATTTCAAGCTTCTTTGAATCTAAATTTGCATCAGGAACATATGACTTTATGCCAGCTCCATCTGACTACATTGATGCATTAAATGCTAAAAATGAACAAGTTGTTTTAAAATCAAATGGTATTGCTTTATCAAAATCTGAAACAGAAGCATATCGTAACGCTTTAAATTCAATGGATAAAGATAGCTTACAATACAAAATTGGTCAATACTGA", "aa": "MFGIDANKLFEKDKFIQKVSAEGNLKDREAITFESINGKEKDANISSFFESKFASGTYDFMPAPSDYIDALNAKNEQVVLKSNGIALSKSETEAYRNALNSMDKDSLQYKIGQY", "aa_hexdigest": "8d4b48fbe150caec5d837c335e394ad8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12782.115199999984, "isoelectric_point": 4.9841203689575195 }, "id": "DFOKDJOJIG_417", "locus": "GCA013367855_00390" }, { "type": "cds", "sequence": "contig_1", "start": 209154, "stop": 209384, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTAAATGAACGTTTGAATGTTCAAACTTCAATCTTTGGATTTGTTAGAACACCATTTATTTCTTCAAAATTAGGTAAAAATTACAAGTTCAATGAAAACTTCGCTAAATTAGTATATGGATCTTCACTAGCTGATATTGACAGTGGAAAAGCAGACACTTCAGAATCATATCTATTTGGTAAAGGTTTAAGCTTCAGAACACTTCTATATGCAGCAGTAAACTGA", "aa": "MKLNERLNVQTSIFGFVRTPFISSKLGKNYKFNENFAKLVYGSSLADIDSGKADTSESYLFGKGLSFRTLLYAAVN", "aa_hexdigest": "90818244ecf9951db069f4944e5f2001", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8480.571899999995, "isoelectric_point": 9.572346305847166 }, "id": "DFOKDJOJIG_418", "locus": "GCA013367855_00391" }, { "type": "cds", "sequence": "contig_1", "start": 209487, "stop": 209801, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATACCATTACAGACTTCAGAGACCAAGCTAACTCACTATTTGCAATTGACAAAGATGGAAACAAAGTTGATTTCGGTGATAAATTAGGTAAGGAATTAAGTCCTTCTGAAAACGCTAAAGCTTCAGAAACTGAATCATCAATTTCTGAAAAGATGAAATCAGCAGGATTTAAAGTTCTTAAAGAAAAAATGAAAGAATTACTAGATGAATTCTTTAAAGAACAAAAAATTACCGATCCCGAAAAACAAACAATTGAATTTGAAGTAGGATTTAGATATTTAAATGCTCCACAACTATACATGAATGCATGA", "aa": "MNTITDFRDQANSLFAIDKDGNKVDFGDKLGKELSPSENAKASETESSISEKMKSAGFKVLKEKMKELLDEFFKEQKITDPEKQTIEFEVGFRYLNAPQLYMNA", "aa_hexdigest": "5e5bdc55fc70ad48d16fb749b95a2b57", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11865.24639999999, "isoelectric_point": 4.922847938537596 }, "id": "DFOKDJOJIG_419", "locus": "GCA013367855_00392" }, { "type": "cds", "sequence": "contig_1", "start": 210006, "stop": 210344, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAGGTTGCACACCAAACAGGTGACCAAAAAACTAAATTTGAAAAAGCCTTCCCAGAAATTGCTAAGTTGGCTAAAACATTGTTAGAATATGCAAAAACAAATAAACCAAATCTATCATTGGATTTTGACGAAATTGATAAAATTGACAATAGATATTTAAATTCAAACTTACCTGCAATTTTAGCATCATATAAATTAGAAAAGGATCCTAAAACCGGAAAATATGTAATTGCTAAAAAAGATGTTAAAGGTTCAAAACCAAAACCAATTGAATTCGATTTTGGAAAAGACAGTTCAGGAAAATCAATAGAACCAACCGATCTATATCAATGA", "aa": "MVKVAHQTGDQKTKFEKAFPEIAKLAKTLLEYAKTNKPNLSLDFDEIDKIDNRYLNSNLPAILASYKLEKDPKTGKYVIAKKDVKGSKPKPIEFDFGKDSSGKSIEPTDLYQ", "aa_hexdigest": "05762d980ea69b951289e2a7fb3f8af8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12637.343699999985, "isoelectric_point": 9.13583087921143 }, "id": "DFOKDJOJIG_420", "locus": "GCA013367855_00393" }, { "type": "cds", "sequence": "contig_1", "start": 210591, "stop": 211049, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAATATATATTACAAAGAATAGCGTTTGCTATTCTTACTCTATTTATTATCTCTATTTTCGTTTATGTGCTGGTTGCTGCATTTGGAAACAACCCAGTTAATGACTTAGCACAAGCACAATTTCAAGCAAGCAAAAAATCAGGATTAACATATACTGAAATATTGCATAAACTTGAAATAGAAAACGGATTAAGATATAAAGATAACCCGGACGTTTTAGTTCCTATAATTGTTAGATATGGCAATTATATGAAAAACATTTTTACGAAGGGCGATTTTGGATTTTTATATAATCCAGCTAATAACCCAAACTCTGCTTTATATACAAATATGGCAAAACTATTCTTTACCCCCCTACAATATTCATTAATAATCACTGTTCCAACATTCTTTATTAGTTCAATATTGGGTATTGCAATAGGTGTCATTGCCGGATATAAGCGTGGAAAATGA", "aa": "MTKYILQRIAFAILTLFIISIFVYVLVAAFGNNPVNDLAQAQFQASKKSGLTYTEILHKLEIENGLRYKDNPDVLVPIIVRYGNYMKNIFTKGDFGFLYNPANNPNSALYTNMAKLFFTPLQYSLIITVPTFFISSILGIAIGVIAGYKRGK", "aa_hexdigest": "ef6b3ef7a0471528051ee3e117150061", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17072.862599999986, "isoelectric_point": 9.63056125640869 }, "id": "DFOKDJOJIG_421", "locus": "GCA013367855_00394" }, { "type": "cds", "sequence": "contig_1", "start": 211056, "stop": 211445, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTTCAAATGTTTTTGTGTTAATATTTGTTGCTATACCATCATTTATATTGGCCCCTATTGTAATAACTATTTCGGTCGCATTAGGAATTTCTAGCGTTGTGCCTAGACCTGGTGAACAATCATTTGGAGTAATGTTTGTTTCTTATTTACCTCCTATATTTGTTATGTCAATAACCTCATTAGCAGGTTATGTAACATATACAAGAAATCAAGTTATTACAGTATTGACATCAAATTTCGTTTTAATAGCTAAAACAAAAGGATTGAGTTCTTCACAAATTTTCTTTAAATATGTTTTAAGAAATATTTCAATTCCTTTATTTAGCCTAGTTTTTGGGTCATTTATTGGTCTATTATCAGGATCAATAATTATTGAACAATATTGA", "aa": "MSSNVFVLIFVAIPSFILAPIVITISVALGISSVVPRPGEQSFGVMFVSYLPPIFVMSITSLAGYVTYTRNQVITVLTSNFVLIAKTKGLSSSQIFFKYVLRNISIPLFSLVFGSFIGLLSGSIIIEQY", "aa_hexdigest": "c44196720190f8b1fd87aec40f105bd6", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14021.567999999983, "isoelectric_point": 9.77445468902588 }, "id": "DFOKDJOJIG_422", "locus": "GCA013367855_00395" }, { "type": "cds", "sequence": "contig_1", "start": 211268, "stop": 211615, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTATTAGTAGAATATTTAATCTTAGGATCTAATATTGCATATGAAACGTCAATAATAATATCGGCAATTAGCGATATAAATGTAAAGAATAATGTAGAGAACATAACAACATTAATTTCTCCTGTAGGGAAGGCATTAACTATTATTTGTGATGTTCCTGGTACTTGTCAATATTGTTCAATAATTATTGATCCTGATAATAGACCAATAAATGACCCAAAAACTAGGCTAAATAAAGGAATTGAAATATTTCTTAAAACATATTTAAAGAAAATTTGTGAAGAACTCAATCCTTTTGTTTTAGCTATTAAAACGAAATTTGATGTCAATACTGTAATAACTTGA", "aa": "MLLVEYLILGSNIAYETSIIISAISDINVKNNVENITTLISPVGKALTIICDVPGTCQYCSIIIDPDNRPINDPKTRLNKGIEIFLKTYLKKICEELNPFVLAIKTKFDVNTVIT", "aa_hexdigest": "3f3787c6c0d4cb7445f61c2608e9dc5a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12785.927199999976, "isoelectric_point": 5.326461601257324 }, "id": "DFOKDJOJIG_423", "locus": "GCA013367855_00396" }, { "type": "cds", "sequence": "contig_1", "start": 211738, "stop": 212364, "strand": "+", "frame": 1, "gene": "dppC", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG1173", "COG:EP", "SO:0001217", "UniRef:UniRef50_D1J7U0", "UniRef:UniRef90_D1J7U0" ], "nt": "ATGAATATAAATGAAGTTAAAGCCTTTAACAAAGAATATAAAATTAATACTGAATTAGAGAAAAAATTTAAATTTGTTGCACCAGAAGATAGAATAGTAATCTCTTCTGTTGCAGGTAAGCCTAAGAAATTAGGTATAGAAATTTTAAAGAGATTTTTTACTAAACCAGTAGTATTAATAGCACTAGCAGTATTTATAATTATTTTACTGTTATCTATATTAGTTCCTGCTAATGGAATTTCTTACTATAAACCAAACGATCCAATTTCAGATAATGCATTTGTAAAGAATTTACCACCTGCATCTTCTCCAATTGTTACTCAAGCATTAGAATCAAATGATCCCGTATTCAAATTTTATCAATTTGTCTTAAATAGGTCAGAAACCGATCCTGTATATAAGGCTAGAATGGCATTTTTCTTAAATGGCTTTCAAGCAAAAGTTAATGATGTTGGGATCTATGTTGCAAAATATAATGCTTATGATTTAGTAGTTGCTAATTACATAAATGGAATTATTTCTAAGAATCAAATAACAGATGCTGAATATTTAAAAATATTTAATGAACAAAAAAACTTCTATTCTCATGTTTTATTAGGAACATCATCAATAGGCTACGATATTTGA", "aa": "MNINEVKAFNKEYKINTELEKKFKFVAPEDRIVISSVAGKPKKLGIEILKRFFTKPVVLIALAVFIIILLLSILVPANGISYYKPNDPISDNAFVKNLPPASSPIVTQALESNDPVFKFYQFVLNRSETDPVYKARMAFFLNGFQAKVNDVGIYVAKYNAYDLVVANYINGIISKNQITDAEYLKIFNEQKNFYSHVLLGTSSIGYDI", "aa_hexdigest": "94b85e1c35d6e9f0936d175bb6a26fd4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dppC" ], "truncated": "3-prime", "pseudogene": { "start": 211738, "stop": 212667, "observations": { "mutation": [ 212362, 212374, 212386, 212539, 212611 ], "stop": [ 212362, 212374, 212386, 212539, 212611 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U0", "query_cov": 1.0, "subject_cov": 0.49056603773584906, "identity": 0.995, "score": 396.0, "evalue": 5.66e-136, "gene_start": 1, "gene_end": 208, "reference_start": 1, "reference_end": 208, "reference_sequence": "MNINEVKAFNKEYKINTELEKKFKFVAPEDRIVISSVAGKPKKLGIEILKRFFTKPVVLIALAVFIIILLLSILVPANGISYYKPNDPISDNAFVKNLPPASSPIVTQALESNDPVFKFYQFVLNRSETDPVYKARMAFFLNGFQAKVNDIGIYVAKYNAYDLVVANYINGIISKNQITDAEYLKIFNEQKNFYSHVLLGTSSIGYDIWTTAWYATWRAIKIALIVVILEAIIGISIGAFLGFYAGKLIDTIIMRVIEIFLAPPTLIWILLFVSIIGANEWSLVLTLTIVGWPGFVGITRMFIITVKDEEFIIAAKAIGVGTPRRIFVHALPTIIGKIANNIVRSVPGVILWIASLAFLGFFKEKKDTNLGQMLIEASQEVGSNIWIIALPTIILLFLSLSLNFIALGVHDALDPRVMNKGKGK" }, "paralog": false, "identity": 0.883495145631068, "score": 513.0, "evalue": 1.36e-184, "description": "Internal stop codon at 212362, 212374, 212386, 212539, 212611. Nonsense mutation around 212362, 212374, 212386, 212539, 212611.", "psc": { "uniref90_id": "UniRef90_D1J7U0", "gene": "DppC", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_D1J7U0", "cog_id": "COG1173", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U0", "UniRef:UniRef50_D1J7U0", "COG:COG1173", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U0" ], "product": "Oligopeptide transport system permease protein" } }, "id": "DFOKDJOJIG_424", "locus": "GCA013367855_00397" }, { "type": "cds", "sequence": "contig_1", "start": 212638, "stop": 212793, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCATTATTACAGTGAAAGATGAAGAATTCATCATTGCTGCAAAAGCAATTGGAGTTGGTACACCAAGAAGAATTTTCGTTCATGCATTACCAACTATAATTGGAAAAATTGCAAATAATATAGTAAGATCAGTACCAGGTGTTATTCTATGA", "aa": "MFIITVKDEEFIIAAKAIGVGTPRRIFVHALPTIIGKIANNIVRSVPGVIL", "aa_hexdigest": "766e71a14c489f6cc7701055b90c904a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5513.6307, "isoelectric_point": 10.266412925720214 }, "id": "DFOKDJOJIG_425", "locus": "GCA013367855_00398" }, { "type": "cds", "sequence": "contig_1", "start": 212857, "stop": 213012, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGAAGCTTCGCAAGAAGTTGGCTCAAATATTTGGATTATTGCTTTGCCTACAATTATTTTATTGTTTTTATCATTATCATTGAATTTCATTGCCTTAGGTGTGCATGATGCACTTGACCCACGTGTAATGAACAAAGGAAAAGGTAAGTAA", "aa": "MIEASQEVGSNIWIIALPTIILLFLSLSLNFIALGVHDALDPRVMNKGKGK", "aa_hexdigest": "7a6ce3add55c99c33ec77b3057c90848", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5548.563800000003, "isoelectric_point": 6.518659400939941 }, "id": "DFOKDJOJIG_426", "locus": "GCA013367855_00399" }, { "type": "cds", "sequence": "contig_1", "start": 213014, "stop": 213388, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGCAAAAATTATTAATAAACACGACAATTTAGATAAAAATAGCTCAGATACTATAAAACATCAAAAAATTAATAAAAGTACTAGAGTTTTAGATTCAATTCGTGTTGAACCTCTAAAAAATAATCAAATATTAAAAGTTAGAAATCTGCATGTTAGTTTCCCTCAAGGTCATAAGAAAAGTATCCATATTGTTAGAGGTGTTGATTTAGATGTCCATAGAGGTGAAATAATTGGCCTCGTTGGAGAATCTGGTTCTGGTAAGTCAGTAACTTCAAAAACTTTAATTAATGTTAATGAATCAGGTATTATAAGTGCTGATCAAATTCAAATTGGCGAATATGAACTAACAAATATTAAAAAACAAAAATATTGA", "aa": "MAKIINKHDNLDKNSSDTIKHQKINKSTRVLDSIRVEPLKNNQILKVRNLHVSFPQGHKKSIHIVRGVDLDVHRGEIIGLVGESGSGKSVTSKTLINVNESGIISADQIQIGEYELTNIKKQKY", "aa_hexdigest": "98db6a6241b8bf1f5873b249a325d459", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 56, "aa_cov": 0.43548387096774194, "hmm_cov": 1.0, "evalue": 1.2752041127626098e-09, "score": 34.4594612121582, "start": 66, "stop": 119 } ], "seq_stats": { "molecular_weight": 13859.722299999989, "isoelectric_point": 9.734097480773926 }, "id": "DFOKDJOJIG_427", "locus": "GCA013367855_00400" }, { "type": "cds", "sequence": "contig_1", "start": 213566, "stop": 213901, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FD1CF63" ], "nt": "TTGCGTTCGGCTGAAAAAATATTTTATGCATACCCTCACACTCTATCAGGGGGTATGAAACAAAGAATTGTTATTACAATGGTTGTTGCTTTGAAGCCAGAATTAATAATCGCTGATGAACCAACAACAGCATTAGACCCAACAGTTCAAGCCAGCGTTTTGGCATTATTTGAAGATATTAGACAAAAAATGGGAATTTCAATAATTTTAATAAGTCACAATATATCTGTTATTGCAAAATTTTGTGATTACATTTATGTTATGTATGCTGGTAGAATTGTTGAAAAAGGAACCAAACAAGAAATCTTTACAAATCCTGCCCACCCATATACTTGA", "aa": "MRSAEKIFYAYPHTLSGGMKQRIVITMVVALKPELIIADEPTTALDPTVQASVLALFEDIRQKMGISIILISHNISVIAKFCDYIYVMYAGRIVEKGTKQEIFTNPAHPYT", "aa_hexdigest": "aa241ded370b8c4f868d7d0398dcf46a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FD1CF63", "query_cov": 0.9099099099099099, "subject_cov": 0.9439252336448598, "identity": 0.525, "score": 119.0, "evalue": 7.69e-33, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000FD1CF63" }, "pscc": { "uniref50_id": "UniRef50_UPI000FD1CF63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FD1CF63" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_428", "locus": "GCA013367855_00401" }, { "type": "cds", "sequence": "contig_1", "start": 213971, "stop": 214090, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATTTACCTCTTGGTGATCCATTTGTATACCGTAATGACTACGCATTAGAAATAGACTATTTAAAAGAACCTCCTCTAATTCCAATTACTGAAACCCATGCTGCCGCAACATGA", "aa": "MANLPLGDPFVYRNDYALEIDYLKEPPLIPITETHAAAT", "aa_hexdigest": "89750929c15eade9b04e273a06f3c46d", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4373.931700000001, "isoelectric_point": 4.290286827087402 }, "id": "DFOKDJOJIG_429", "locus": "GCA013367855_00402" }, { "type": "cds", "sequence": "contig_1", "start": 214173, "stop": 214589, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "nt": "ATGAATAATAAAAATTTTAAAGATAAAAAAGTTATCTTATCGATAGATAATCTAAAAAAATACTTCGTTAATCAAGGAATGATAAATAAGGCTGTTGATGGAGTTTCTTTTGATGTACATGAAGGAGAAATAGTTGGTTTAATTGGAGAATCAGGTTCAGGTAAAACAACTGTTGGTTCAACAATACTTAGACTATATGATGATTACAACGGATTTGTAAGATTAGAAGACAAAATAATTTCAGGTAAGCGCATTAGTGAATCTTTAAATCGTTTTTTAAGAAAAAATGTGCAAATGATTTTCCAAGATCCACATGCTTCATTAAATAGTCAACAAAATATTTATTCAATTTTAAAAGAACCTTTATTAGTTAATGGAGTAATGAAGGACAAAATTAAGGATATTTTTAAAGATTGA", "aa": "MNNKNFKDKKVILSIDNLKKYFVNQGMINKAVDGVSFDVHEGEIVGLIGESGSGKTTVGSTILRLYDDYNGFVRLEDKIISGKRISESLNRFLRKNVQMIFQDPHASLNSQQNIYSILKEPLLVNGVMKDKIKDIFKD", "aa_hexdigest": "91715e0a19db33f76a755f8881eb8d8a", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00249F2F44", "query_cov": 0.9202898550724637, "subject_cov": 0.9921875, "identity": 0.701, "score": 184.0, "evalue": 1.13e-57, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000CA0BF5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000CA0BF5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_430", "locus": "GCA013367855_00403" }, { "type": "cds", "sequence": "contig_1", "start": 214818, "stop": 215987, "strand": "+", "frame": 3, "gene": "dppD", "product": "Oligopeptide transport ATP-binding proteinhomolog", "db_xrefs": [ "COG:COG0444", "COG:EP", "SO:0001217", "UniRef:UniRef50_B3PML2", "UniRef:UniRef90_D1J7U2" ], "nt": "ATGTATGCAAATGTTGATAGGTTAATTGAATTTTATTATGAAACACAACGTAAATATCGTAATAAAGAATTACCTGGACCACAAATTGCTTTAAATGAAAAAGAAGAAAAATTAAAATACATTCAATGTTTAAGCAAGACTTCAAAGATTGCTTATGAAGCTTCACTTGAATTAAAAGAAGTTAAAAAACAAATATTTAATTTAAGACATAAAATTGAAAAAATATCTTCTGAAAGCAAGAATACATTTGCAAACTATATTCATGAATCAAAAAATGAAAAGAGTTTAATTGACATAGCAAGATTAATGTCATGTGATTTAGACTTTTATTCATATAATTTAAAAAGTGAATTGTTGATGAATAAAAGGGGCCAAGTATTGGCAAAAATAAAACCATTTAATAAATTCCTAGGATTTAATAATATAAAGAAGTTAATTAATGAACTAGATGAATATATTAATGAATTTTATAAAACAAAATTAGCCCCATTGCCATATTCAAAAACATTGAAATCTGATATTAAAAACGTTTTAGAAAAAGACTTTGATTTTAAATTCGATGAATATACTAAAATATCAGAATTAGAAAAACAAAAGTTAGATTCAGAATTAAAACAATTACTATCAAGAGAAGTTGAAATTAAAAAAGTAATAAAAGAAAATCCAAATCCTGAAATTACTTCAGAACAACTTCAAGAAGCAAAAAACGATCTAGAATTAGGAAAACAAGCATATCTTGAAGGCAGATCTAAATATTTAATTTCATATAAAGCCGAACTTGAAAGCTTGTATAAAAATCTTGAAGAACAAAAGAAATTCTATCTTGAATTGCGCAAGCAACAAGATTATTGCAATAACAAATATAAAGAATTGAAACAAAAATTCTTTGAATATGTTGAACAATTACGTCAAAAAGAAAAAGATAAGATTTTATTAAAAATAGCTGAATTAAAACAAAATCAAGACAAATCAAACAAAGATAAAAAGTCATTAGCAAAATTACAAAGCAAATTAAAATCAATTAAGGTTCAATACAATACAATATTAAAAATGTACCATTCAGATATTTCATTAAAAGAAGATACATTAAAATCATTTGATATTGAAAGAAAATATCTTGATAAAGATATAAATAATATTTATGTTTTATTAGGAATTGACCACAAGTGA", "aa": "MYANVDRLIEFYYETQRKYRNKELPGPQIALNEKEEKLKYIQCLSKTSKIAYEASLELKEVKKQIFNLRHKIEKISSESKNTFANYIHESKNEKSLIDIARLMSCDLDFYSYNLKSELLMNKRGQVLAKIKPFNKFLGFNNIKKLINELDEYINEFYKTKLAPLPYSKTLKSDIKNVLEKDFDFKFDEYTKISELEKQKLDSELKQLLSREVEIKKVIKENPNPEITSEQLQEAKNDLELGKQAYLEGRSKYLISYKAELESLYKNLEEQKKFYLELRKQQDYCNNKYKELKQKFFEYVEQLRQKEKDKILLKIAELKQNQDKSNKDKKSLAKLQSKLKSIKVQYNTILKMYHSDISLKEDTLKSFDIERKYLDKDINNIYVLLGIDHK", "aa_hexdigest": "8e467af0c96eb13097d60070f53b80ed", "start_type": "ATG", "rbs_motif": null, "genes": [ "dppD" ], "truncated": "both", "pseudogene": { "start": 214518, "stop": 216290, "observations": { "mutation": [ 214587, 214698, 215985, 216054 ], "stop": [ 214587, 214698, 215985, 216054 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U2", "query_cov": 1.012853470437018, "subject_cov": 0.4651711924439197, "identity": 0.982, "score": 712.0, "evalue": 4.18e-251, "gene_start": 1, "gene_end": 389, "reference_start": 216, "reference_end": 609, "reference_sequence": "MNNKNFKDKKVILSIDNLKKYFVNQGMINKAVDGVSFDVHEGEIVGLIGESGSGKTTVGSTILRLYDDYNGFVRLEDKIISGKRISESLNRFLRKNVQMIFQDPHASLNSQQNIYSILKEPLLVNGVMKDKIKDIFKDWLNVKKNFKYTFQIEAMRLELQNYLEINKLAEPLFKKWTENLNKLEFNFDISREDNFSLFFGYLEEKQNVESIIINNMYANVDRLIEFYYETQRKYRNKELPGPQIALNEKEEKLKYIQCLSKTSKIAYEASLELKEVKKQIFNLRHKIEKISSESKNTFANYIHESKNEKSLIDIARLMSCDLDFYSYNLKSELLMNKRGQVLAKIKPFNKFLGFNNIKKLINELDEYINEFYKTKLAPLPYSKTLKSDIKNVLEKDFDFKFNEYTKISELEKQKLDSELKQLLSREVEIKKVIKENPNPEITSEQLQEAKNDLELGKQAYLEGRSKYLISYKAELESLYKNLEEQKKFYLELRKQQDYCNNKYKELKQKFFEYVEQLRQKEKDKILLKIAELKQNQDKSNKANNINLKKSLAKLQSKLKSIKVQYNTILKMYHSDISLKEDTLKSFDIERKYLDKDINNIYVLLGIDHKWVETNLKTADAMAGVDAKHMKWVWRKRYFDSKVSYPIAKLLISSILYKTIIYKALEDVGLLKQFAYRYPHEFSGGQLQRIVIARALIVEPQVIVADEPIASLDISIQAQVVNLLKELCIKKNIGLIFIAHDLSMIEYVADEVQIMHLGKIVESGKTEAIYANPIHPYTINLFKAIPKISNANEKFQNVSFALDYLDEQKFPNVPETFKVGEDHYVYGTASQLKQWTHNAKLEKEHKEL" }, "paralog": false, "identity": 0.9815126050420168, "score": 1128.0, "evalue": 0.0, "description": "Internal stop codon at 214587, 214698, 215985, 216054. Nonsense mutation around 214587, 214698, 215985, 216054.", "psc": { "uniref90_id": "UniRef90_D1J7U2", "gene": "DppD", "product": "Oligopeptide transport ATP-binding proteinhomolog", "uniref50_id": "UniRef50_B3PML2", "cog_id": "COG0444", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U2", "UniRef:UniRef50_B3PML2", "COG:COG0444", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_B3PML2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PML2" ], "product": "Oligopeptide ABC transporter ATP-binding protein" } }, "id": "DFOKDJOJIG_431", "locus": "GCA013367855_00404" }, { "type": "cds", "sequence": "contig_1", "start": 216120, "stop": 216659, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "nt": "TTGTATAAAACAATAATTTATAAAGCATTGGAAGATGTTGGACTATTAAAACAATTTGCTTATCGTTATCCTCATGAATTTAGTGGGGGTCAATTGCAAAGAATTGTTATTGCAAGAGCATTGATAGTTGAACCTCAAGTTATTGTGGCCGATGAACCTATTGCCTCACTAGATATATCAATTCAAGCGCAAGTTGTTAACTTGCTAAAAGAATTATGTATTAAAAAGAACATCGGTCTTATATTCATAGCACACGACCTTTCAATGATTGAATATGTTGCTGATGAAGTTCAAATAATGCACCTTGGAAAAATTGTAGAAAGTGGAAAAACCGAAGCAATATATGCAAATCCAATTCACCCATATACAATAAACTTATTTAAAGCTATTCCTAAAATTTCTAATGCTAATGAAAAATTCCAAAATGTTTCATTTGCTTTAGATTACTTAGATGAACAAAAATTCCCTAATGTTCCTGAAACATTTAAAGTTGGTGAAGATCACTATGTTTATGGAACTGCTAGTCAACTTAAACAATGA", "aa": "MYKTIIYKALEDVGLLKQFAYRYPHEFSGGQLQRIVIARALIVEPQVIVADEPIASLDISIQAQVVNLLKELCIKKNIGLIFIAHDLSMIEYVADEVQIMHLGKIVESGKTEAIYANPIHPYTINLFKAIPKISNANEKFQNVSFALDYLDEQKFPNVPETFKVGEDHYVYGTASQLKQ", "aa_hexdigest": "f1efd8141ea362f19edc8aad725ebec4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024CC3F60", "query_cov": 1.0, "subject_cov": 0.8647342995169082, "identity": 0.737, "score": 267.0, "evalue": 7.16e-89, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A4R0XTG3" }, "pscc": { "uniref50_id": "UniRef50_A0A4R0XTG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_432", "locus": "GCA013367855_00405" }, { "type": "cds", "sequence": "contig_1", "start": 216770, "stop": 217492, "strand": "-", "frame": 3, "gene": "rsuA", "product": "Pseudouridine synthase", "db_xrefs": [ "COG:COG1187", "COG:J", "RefSeq:WP_112636522.1", "SO:0001217", "UniParc:UPI000DCD3F91", "UniRef:UniRef100_UPI000DCD3F91", "UniRef:UniRef50_D1J7U3", "UniRef:UniRef90_D1J7U3" ], "nt": "ATGGAAGAAAAAGCAAAACGAATTCAAAAAATTTTAAGTGAGCAAGGCATTTGTTCTAGAAGAAAAGCAGAAGAATTAATTTTATCGGGGAAGATTTTAATAAATAATAAAATTGCAAAATTAGGCGAATGTGTAACTATAAATGACGAAATTAAAATAAACAATAGAATTATCAAATTAAATAATAATGTTGCAAATAATTTTAAATACATTGTTTTAAATAAACCCATGAATACAATTTGTACAGCAAGTGATCCTCAAAATAGAAAAACAATTTACGAATATCTTAAAGCAAAAGAACGCTTATTTTCAATAGGAAGATTAGACTTTAATACAACAGGAATTATATTAATCACAAACGATGGAGAATTTGCTAATTTATTAAGCCATCCTTCTTCTGAAATTCCTAGAATTTATATTGCTACATTAGAACGTGAAATGCTTGATGGTGAATTAAACTTATTAAATTCAAATCACGTATTATTAAATGGCAAAATTTCAAAACAAAAAGTTACAAAATTAAGTGCTTTTAAATACGAAGTTCTACTATATGAAGGCAGAAACCATCACGTTAAAAATCTATTTTTGTTAGTAAATAATTATGTTAAAAAATTACATAGAAAATCTTTTGGATGCATTGATGATAGTAATTTAAAAATAGGTCAATATAGAAATTTAACTTCAAGCGAAATTAATAAATTATTAGCTTATATAAAGAAATAA", "aa": "MEEKAKRIQKILSEQGICSRRKAEELILSGKILINNKIAKLGECVTINDEIKINNRIIKLNNNVANNFKYIVLNKPMNTICTASDPQNRKTIYEYLKAKERLFSIGRLDFNTTGIILITNDGEFANLLSHPSSEIPRIYIATLEREMLDGELNLLNSNHVLLNGKISKQKVTKLSAFKYEVLLYEGRNHHVKNLFLLVNNYVKKLHRKSFGCIDDSNLKIGQYRNLTSSEINKLLAYIKK", "aa_hexdigest": "2f267adf24b6f89de8de5935f6337dd1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000DCD3F91", "ncbi_nrp_id": "WP_112636522.1", "uniref100_id": "UniRef100_UPI000DCD3F91", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DCD3F91", "RefSeq:WP_112636522.1", "UniRef:UniRef100_UPI000DCD3F91" ] }, "ips": { "uniref100_id": "UniRef100_UPI000DCD3F91", "uniref90_id": "UniRef90_D1J7U3" }, "psc": { "uniref90_id": "UniRef90_D1J7U3", "gene": "RsuA", "product": "Pseudouridine synthase", "uniref50_id": "UniRef50_D1J7U3", "cog_id": "COG1187", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J7U3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U3" ], "product": "Pseudouridine synthase" }, "genes": [ "rsuA" ], "id": "DFOKDJOJIG_433", "locus": "GCA013367855_00406" }, { "type": "cds", "sequence": "contig_1", "start": 217600, "stop": 217854, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U4", "UniRef:UniRef90_D1J7U4" ], "nt": "ATGGAATATTTACAAAATATAGACTTAAGCAAAATAAGATCGCTTTGTTTACAAAAAAAGCGGGAATTTATCTTTAATTATTGCCTTTTTGAGCAGCTAAAAGATAAACAATTAAAACAAAAAAAATCACAAACAATCAATGACGAAGAAGATGATGATATTTCAAAATTAGAAAAATTTATTAAATTATTAAATTCTGATAATCAAAAAATCTTTTTTAATAATTTTATTTATAACAATCCTGAATCTGAATGA", "aa": "MEYLQNIDLSKIRSLCLQKKREFIFNYCLFEQLKDKQLKQKKSQTINDEEDDDISKLEKFIKLLNSDNQKIFFNNFIYNNPESE", "aa_hexdigest": "cc38cd88563039e12c5649d4cb46963b", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 217600, "stop": 217938, "observations": { "mutation": [ 217852, 217867 ], "stop": [ 217852, 217867 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U4", "query_cov": 1.0, "subject_cov": 0.75, "identity": 1.0, "score": 162.0, "evalue": 1.84e-50, "gene_start": 1, "gene_end": 84, "reference_start": 1, "reference_end": 84, "reference_sequence": "MEYLQNIDLSKIRSLCLQKKREFIFNYCLFEQLKDKQLKQKKSQTINDEEDDDISKLEKFIKLLNSDNQKIFFNNFIYNNPESEWYLAYWSKNAYYKKLNYIVNLFIDFINQ" }, "paralog": false, "identity": 0.9821428571428571, "score": 221.0, "evalue": 3.62e-76, "description": "Internal stop codon at 217852, 217867. Nonsense mutation around 217852, 217867.", "psc": { "uniref90_id": "UniRef90_D1J7U4", "uniref50_id": "UniRef50_D1J7U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U4", "UniRef:UniRef50_D1J7U4" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10245.543599999992, "isoelectric_point": 5.506072807312011 }, "id": "DFOKDJOJIG_434", "locus": "GCA013367855_00407" }, { "type": "cds", "sequence": "contig_1", "start": 218118, "stop": 219002, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9K8", "UniRef:UniRef50_D1J7U5", "UniRef:UniRef90_A0A454C9K8", "UniRef:UniRef90_D1J7U5" ], "nt": "TTGAATTACGACCAAGAAATAGATGATATAGATGTTAAATGTTCGATTAACAATCAATTATTGAAACTCCAAAAAGTCTTTGATTCAGATAAACATGAATATACATATACTCTTATTTCTGAATTTAATGAGGCAACAAAAACTAAAAAAGTTCAGGACATAAAAAATATTAATTTTGAAATTTTTTATAAGGTAAAAGGTTTATTGTATCAAGTAGAAAGATATACATATGAGATATATAGCAATAACAATTTAAACAGAGAACTAGTTTCAAATAAAAATGGTGTCGAATATGATTTATGGACTAAACTAAGAATTATGTCATTAGCAGATAGAGACATTCCAACAGATATGGAACATTATTCATTTGTTGAACAAGTTAAATACAAATTTATACCAACTACTCTGGTGTTGGGAAAACATAATAAAAAATTATTTGATGTTGAGATATCAAAGAAAGAAATTTCTAACAAAAGTGGCATAATAAGCGATTTTTCTCAACTAATTCAAAGCGATATTAATGGTTTTAATGTATCTATTGATATTGATAAACAATTTATAAAAGGCAACTTGAATGCAAAAATTAATGATTTCAGTAAAAATGAGCATAAGCAAATTTCAATAGATTCTTATTCATATTATGACAAGGTTTCTAAGGCAATAATAGCAAATAATGATAGTATTGGCGCAGCTAAAGGCTTATTGTTGCCTCTTAATTATTCAGGAAATTATTCTTATTTACTAAATATTAATTTTGGTGAGGATTTGAAAGGTTTTAAAATTAGATACAATCAAGAAATTTCAAAACCATTTTTTAATGAAAAAAGTGGCTTAGTAAAATTAAATGTAAATAATATATTTAAACAACAAAATTTCATAAAATGA", "aa": "MNYDQEIDDIDVKCSINNQLLKLQKVFDSDKHEYTYTLISEFNEATKTKKVQDIKNINFEIFYKVKGLLYQVERYTYEIYSNNNLNRELVSNKNGVEYDLWTKLRIMSLADRDIPTDMEHYSFVEQVKYKFIPTTLVLGKHNKKLFDVEISKKEISNKSGIISDFSQLIQSDINGFNVSIDIDKQFIKGNLNAKINDFSKNEHKQISIDSYSYYDKVSKAIIANNDSIGAAKGLLLPLNYSGNYSYLLNINFGEDLKGFKIRYNQEISKPFFNEKSGLVKLNVNNIFKQQNFIK", "aa_hexdigest": "3ae1ef6e8440493aa1ad17a3156d4c38", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7U5", "query_cov": 0.8163265306122449, "subject_cov": 0.8, "identity": 0.975, "score": 456.0, "evalue": 6.35e-160, "valid": true, "uniref50_id": "UniRef50_D1J7U5" }, "pscc": { "uniref50_id": "UniRef50_D1J7U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U5" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 217938, "stop": 219083, "observations": { "mutation": [ 218037, 219000 ], "stop": [ 218037, 219000 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9K8", "query_cov": 1.0, "subject_cov": 0.7716535433070866, "identity": 0.986, "score": 564.0, "evalue": 1.93e-201, "gene_start": 1, "gene_end": 294, "reference_start": 61, "reference_end": 354, "reference_sequence": "MFPIVMQAINDQEPTKWIENINAYTTKQLYLDKWTNSFQKVDFKLQRLIENNKFIIEFKYLNYDQEIDDIDVKCSINNQLLKLQKVFDSDKHEYTYTLISEFNEATKTKKVQDIKNINFEIFYKVKGLLYQVERYTYEIYSNNNSNRELVSNKNGVEYDLWTKLRILSLADRDIPTDMEHYSFVEQVKYKFMPTTLVLGKHNKKLFDVEISKKEISNKSGIISDFSQLIQSDINGFNVSIDIDKQFIKGNLNAKINDFSKNEHKQISIDSYSYYDKVSKAIIANNDSIGAAKGLLLPLNYSGNYSYLLNINFGEDLKGFKIRYNQEISKPFFNEKSGLVKLNVNNIFKQQNFIKWSVVKAEDIRSLINNSNTLEELMKGGK" }, "paralog": false, "identity": 0.9868766404199475, "score": 747.0, "evalue": 3.45e-276, "description": "Internal stop codon at 218037, 219000. Nonsense mutation around 218037, 219000.", "psc": { "uniref90_id": "UniRef90_A0A454C9K8", "uniref50_id": "UniRef50_A0A454C9K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9K8", "UniRef:UniRef50_A0A454C9K8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9K8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 34278.48329999999, "isoelectric_point": 8.250293159484862 }, "id": "DFOKDJOJIG_435", "locus": "GCA013367855_00408" }, { "type": "cds", "sequence": "contig_1", "start": 219083, "stop": 219427, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U6", "UniRef:UniRef90_D1J7U6" ], "nt": "ATGTTGATTGCAAAAAAGAAAAAAATAATTTTCGGTTTATCTCTTGGATTAGCTTGTGCCGTTGGCGCTGGCGCATTAGTTGGAGTTTATTTTTTAGCATCTTCTAATTCAAATAAAAAAGCTAATAATCTAGACAATAAAAAGGTTAACAAAAAAGATTCAACAAATAACAATAGTGACAACAAAAACATTAATTCTGGCAATGAAAATGAAAAAGATAACACCATTGATAACCAAAACGAAGATAGTAAAATTGAAGATTCTAATGAAGATGGTCAAGCGAATGATGACGATAAAAAGCTTAAACCAGAAACAAGGCCAATTATAAAAACAGAGATTAATTGA", "aa": "MLIAKKKKIIFGLSLGLACAVGAGALVGVYFLASSNSNKKANNLDNKKVNKKDSTNNNSDNKNINSGNENEKDNTIDNQNEDSKIEDSNEDGQANDDDKKLKPETRPIIKTEIN", "aa_hexdigest": "a1ee6110863952bbad4efc53813beb05", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 219083, "stop": 219682, "observations": { "mutation": [ 219425, 219623 ], "stop": [ 219425, 219623 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U6", "query_cov": 1.0175438596491229, "subject_cov": 0.5771144278606966, "identity": 0.948, "score": 142.0, "evalue": 7.55e-41, "gene_start": 1, "gene_end": 114, "reference_start": 1, "reference_end": 116, "reference_sequence": "MLIAKKKKIIFGLSLGLACAVGAGALVGVYFLASSNSNKKANNLDNKKVNKKDSTNNNSDNKNINSGNENEKDNTIDNQNEDSKIEDSNEDGQANDDDKKLKPEVKPRPNIKTEINWREIFPDVKTSDYYNQLNFKDGQGFIDKEMITYIIKDILNRMTILDGDVSYAYKQEDDQNLYIAFKWENKSQKSNITYKFSVNRI" }, "paralog": false, "identity": 0.9601990049751243, "score": 323.0, "evalue": 1.78e-114, "description": "Internal stop codon at 219425, 219623. Nonsense mutation around 219425, 219623.", "psc": { "uniref90_id": "UniRef90_D1J7U6", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J7U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U6", "UniRef:UniRef50_D1J7U6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U6" ], "product": "Lipoprotein" } }, "id": "DFOKDJOJIG_436", "locus": "GCA013367855_00409" }, { "type": "cds", "sequence": "contig_1", "start": 219512, "stop": 219625, "strand": "+", "frame": 2, "gene": null, "product": "MHO_1590 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A6F3B0", "UniRef:UniRef90_UPI0035A6F3B0" ], "nt": "ATGATTACTTATATAATAAAAGATATTTTAAATAGAATGACTATTTTAGATGGCGATGTTTCTTATGCTTATAAACAAGAGGATGATCAAAATTTATATATTGCTTTTAAATGA", "aa": "MITYIIKDILNRMTILDGDVSYAYKQEDDQNLYIAFK", "aa_hexdigest": "ec0392135fa72af3ea93be49d66aeba0", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 219434, "stop": 219682, "observations": { "mutation": [ 219623 ], "stop": [ 219623 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0035A6F3B0", "query_cov": 1.0, "subject_cov": 0.45121951219512196, "identity": 0.973, "score": 75.9, "evalue": 2.96e-17, "gene_start": 1, "gene_end": 37, "reference_start": 27, "reference_end": 63, "reference_sequence": "MFPDVKTSDYYNQLNFKDGQGFIDKEMITYIIKDILNRMTILDGNVSYAYKQEDDQNLYIAFKWENKSQKSNITYKFSVNRI" }, "paralog": false, "identity": 0.9634146341463414, "score": 162.0, "evalue": 9.05e-54, "description": "Internal stop codon at 219623. Nonsense mutation around 219623.", "psc": { "uniref90_id": "UniRef90_UPI0035A6F3B0", "product": "MHO_1590 family protein", "uniref50_id": "UniRef50_UPI0035A6F3B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0035A6F3B0", "UniRef:UniRef50_UPI0035A6F3B0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0035A6F3B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A6F3B0" ], "product": "MHO_1590 family protein" } }, "id": "DFOKDJOJIG_437", "locus": "GCA013367855_00410" }, { "type": "cds", "sequence": "contig_1", "start": 219691, "stop": 219915, "strand": "+", "frame": 1, "gene": "acpP", "product": "Acyl carrier protein", "db_xrefs": [ "COG:COG0236", "COG:I", "RefSeq:WP_020002664.1", "SO:0001217", "UniParc:UPI0002DDE617", "UniRef:UniRef100_A0A2K9YT00", "UniRef:UniRef50_A0AA43R1D2", "UniRef:UniRef90_A0A2K9YT00" ], "nt": "ATGGAATCTGAAAAAATTATAATTAATGCTATTAAAAAACTAACTAAAACAAAATTTAATAAAGAAACTTTTATTCGTGATTTAAATATTGATAGTTTAGATTTGGTTATACTTGTTAGTGATTTAGAAGAGGCTTACAATATAAAAATAAGTGATGAAGAATTGCTTTCATTAAAGACCATAAATGATATTATTGAATTATTTGATAAAAAAACGAATAAATAA", "aa": "MESEKIIINAIKKLTKTKFNKETFIRDLNIDSLDLVILVSDLEEAYNIKISDEELLSLKTINDIIELFDKKTNK", "aa_hexdigest": "0fda9036b020adeab1537197b28e01cc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002DDE617", "ncbi_nrp_id": "WP_020002664.1", "uniref100_id": "UniRef100_A0A2K9YT00", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002DDE617", "RefSeq:WP_020002664.1", "UniRef:UniRef100_A0A2K9YT00" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YT00", "uniref90_id": "UniRef90_A0A2K9YT00" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YT00", "gene": "AcpP", "product": "Acyl carrier protein", "uniref50_id": "UniRef50_A0AA43R1D2", "cog_id": "COG0236", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_A0AA43R1D2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA43R1D2" ], "product": "Acyl carrier protein" }, "genes": [ "acpP" ], "id": "DFOKDJOJIG_438", "locus": "GCA013367855_00411" }, { "type": "tRNA", "sequence": "contig_1", "start": 219948, "stop": 220023, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 66.8, "nt": "ATGGGTATAGTTCAATCGGCAGAACAACGGGCTTCAACCCCGTGTGTTGTGAGTTCAAGTCTTACTACCCATGCCA", "db_xrefs": [], "id": "DFOKDJOJIG_15", "locus": "GCA013367855_00412" }, { "type": "tRNA", "sequence": "contig_1", "start": 220129, "stop": 220203, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 76.0, "nt": "GCCGTCATAGCTCAGGGGCAGAGCACTTCCATGGTAAGGAAGGGGTCGTTGGTTCAATTCCAATTGACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 220169, 220171 ], "id": "DFOKDJOJIG_32", "locus": "GCA013367855_00413" }, { "type": "cds", "sequence": "contig_1", "start": 220313, "stop": 221392, "strand": "+", "frame": 2, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0006415", "GO:0016149", "KEGG:K02835", "RefSeq:WP_047207617.1", "SO:0001217", "UniParc:UPI0006402231", "UniRef:UniRef100_UPI0006402231", "UniRef:UniRef50_A5IZH6", "UniRef:UniRef90_D1J7U7" ], "nt": "ATGGAAAAGACAATGTACGATTCACTACTTTCAATTAAAAATCAATATGATGAATTAACTGAAAAATTAAACGACCCTAATATTTATAATGATATAAAAGCATATACAAGAATAGCAAAAGAAAAATCTGAGTTGCAAGAAATTTATGATGCTTTCACAAGATATCAAAACATTGAAGAATTATATTTCTTGTCAAAAGAAATTTTAACAAACGAAAAAGATCAAGAATTAATTGAACTTGCAAAAAACGACATTGACAATCAAATGAATCAATTGCAAGAAATAGAAGAAGAAATTAAAGAACTTTTATTGCCACAAGATGAAAATGACAAACGTGATGTTATTATGGAAATAAGAGGTGCAGCTGGTGGTGATGAAGCAAATATCTTTTCTGGTGATTTATTTAAAATGTATCAAAAATATGCAGAAATCAAGGGCTTTAAAATCAAGATAATTGATTCTACATATGGTGCTGCCGGAGGATATAGCCAAATTGTTTTTAGTATAAAAGGCGAAAAAGTTTTTTCACAACTAAAATTTGAACGTGGTGTACATAGAGTTCAACGTGTTCCTGCAACTGAAACTCAAGGGCGTGTTCATACATCAACTGCAACTGTAACTGTATTGCCCGAAGTTGATGAAGACGTAAAAATTGAAATAAGACCAGAAGATATAGAAGTTAATGTATTTAGAAGTTCTGGAGCTGGCGGACAATCTGTTAATACAACGGACTCTGCAGTAAGAATTACTCATAAGCCTACAGGATTGGTTGTAACATCACAAGATGAAAGAAGCCAAATTCAAAATAAAGAAACAGCCTTAAAAGTTTTAAAAAGTAGATTGTATGAACTAGAATTAAGAAAACGTGAAGAAGCAGAAAGCGGACTTAGAAAACTAGCTGGAACAGGGGATAGATCAGAAAAAATTAGAACATATAACTATCCTCAAGATAGAATTACAGATCATAGAATTGGCTTTTCAACTTCATTAAAAGTAGTTATGGAAGGCGAATTAGACAAAATTATTGAAGCATTAATAGCTGATGAAAAGGCTAAAAAAATTATTGAAGCGGGAAATTAA", "aa": "MEKTMYDSLLSIKNQYDELTEKLNDPNIYNDIKAYTRIAKEKSELQEIYDAFTRYQNIEELYFLSKEILTNEKDQELIELAKNDIDNQMNQLQEIEEEIKELLLPQDENDKRDVIMEIRGAAGGDEANIFSGDLFKMYQKYAEIKGFKIKIIDSTYGAAGGYSQIVFSIKGEKVFSQLKFERGVHRVQRVPATETQGRVHTSTATVTVLPEVDEDVKIEIRPEDIEVNVFRSSGAGGQSVNTTDSAVRITHKPTGLVVTSQDERSQIQNKETALKVLKSRLYELELRKREEAESGLRKLAGTGDRSEKIRTYNYPQDRITDHRIGFSTSLKVVMEGELDKIIEALIADEKAKKIIEAGN", "aa_hexdigest": "b8cf6fa6e889ab0d438558909693b374", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0006402231", "ncbi_nrp_id": "WP_047207617.1", "uniref100_id": "UniRef100_UPI0006402231", "db_xrefs": [ "SO:0001217", "UniParc:UPI0006402231", "RefSeq:WP_047207617.1", "UniRef:UniRef100_UPI0006402231" ] }, "ips": { "uniref100_id": "UniRef100_UPI0006402231", "uniref90_id": "UniRef90_D1J7U7" }, "psc": { "uniref90_id": "UniRef90_D1J7U7", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A5IZH6", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_A5IZH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IZH6" ], "product": "Peptide chain release factor 1" }, "genes": [ "prfA" ], "id": "DFOKDJOJIG_439", "locus": "GCA013367855_00414" }, { "type": "cds", "sequence": "contig_1", "start": 221392, "stop": 221784, "strand": "+", "frame": 1, "gene": null, "product": "methyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022080B54" ], "nt": "ATGATAGATAAAGAAATTCTACTACGCGAAAAAGAAAGATATAATTTACCCCTTTATATTTCAAAAAAAGAATTAAGAATGCTTAAAAAAGATGTTCCTGTGCAAAAAATAATTGGATACCAGGAATTTCAAAATGTAATTTTGAATTTAAAATATAAAACCTTGATTCCTAGATACGAAACAGAAGAGGTTATTTTAGCTGCATATCCATTTATAAAAAAAGAATCTAAAGTATTGGATTTAGGGTGCGGTAGTGGCCTAATAGGACTTGCAATTAAAAAAAATAAAGACGCTAATGTTACATTAAGTGATATTAGCCGTTTTGCAATCAAACAAACTAAAATAAATGCCAAATTAAATAATTTAAATGTAAATGTAATTCGTTCAAATTGA", "aa": "MIDKEILLREKERYNLPLYISKKELRMLKKDVPVQKIIGYQEFQNVILNLKYKTLIPRYETEEVILAAYPFIKKESKVLDLGCGSGLIGLAIKKNKDANVTLSDISRFAIKQTKINAKLNNLNVNVIRSN", "aa_hexdigest": "fb92bb821032a6ab69fada4c0e251a7f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0022080B54", "query_cov": 0.8846153846153846, "subject_cov": 0.9274193548387096, "identity": 0.513, "score": 94.4, "evalue": 1.82e-22, "valid": false, "product": "methyltransferase domain-containing protein", "uniref50_id": "UniRef50_UPI0022080B54" }, "pscc": { "uniref50_id": "UniRef50_UPI0022080B54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022080B54" ], "product": "methyltransferase domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_440", "locus": "GCA013367855_00415" }, { "type": "cds", "sequence": "contig_1", "start": 221890, "stop": 222099, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTAGAGACAACGGTTTATTTGCTTATAAAAAGATTTTAAAATCTGCATTTAACTATTTAAACGAAAAAGGAATAATTATTTTTGAAGTCGATCCTTATTGCTCTTTATGGTTTAAAAATAAATTTCCAAATGTACTAATACTAAAAGATATAAACAATAAAGATAGGATAGCATTGCTAGCAAAAAATGATTTAAAAAATTAG", "aa": "MFARDNGLFAYKKILKSAFNYLNEKGIIIFEVDPYCSLWFKNKFPNVLILKDINNKDRIALLAKNDLKN", "aa_hexdigest": "e99f8748d453c079e19efaab39262372", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8112.4699999999975, "isoelectric_point": 9.519095420837402 }, "id": "DFOKDJOJIG_441", "locus": "GCA013367855_00416" }, { "type": "cds", "sequence": "contig_1", "start": 222134, "stop": 223246, "strand": "+", "frame": 2, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "EC:1.8.4.-", "GO:0005515", "GO:0005524", "GO:0006457", "GO:0009408", "SO:0001217", "UniRef:UniRef50_A0A448ZV77", "UniRef:UniRef90_D1J7U9" ], "nt": "ATGGAAAATAAACGTGATTATTATGAAGTTTTAGGCATTTCTAAAAATGCCACAGAAAAAGAAATCAAGTCGGCATATAGAAAATTAGCAATGCAATACCACCCAGATAGAAACAAAGCGCCAGATGCTGAAGAAAAATTTAAAGAAGTTTCAGAAGCATATGAAGTTTTATCTGACCCTGAAAAAAGACAAAAATATGATAAGTTCGGACATTCTGCATTCGACCAAAGTTCATTCGGATATTCAGAAGATGTATTTAATAGTTTCTTTAATTCTTTTAGAGATATGTTTTCTGGCGGAGGTTTTGAAGTTGATTCAAGTGATGATTCAGATGATCCTTTTTCAAGTATTTTTGGTTTTGGCTCAAATAGATCTAGAAAAACAAAGGGCAGTGATTTGCAAATGAAATTGTCAATTGATTTTGTAGATTCAATATTTGGAAAGAATGTAACTGTTCAATTAAAGAAATATAGCGAATGTTCACATTGTCATGGTTCAAGAGCAGAAAGTGATAGTGATATTGTTTCATGTCCTCAATGCCATGGTAGTGGTCAAGTTCAACAAAAAATGGGTTTCTTTTCAACAATTAGTCCTTGCAACAAGTGTGGAGGCTTGGGTAAAAGCATTAAAAACACATGCCATGTATGTAGTGGAAAAGGACATGAATATAAATTAGTGAATGAAAATATTCAAATTCCTGGCGGAATTAATAATGGCGAATATATTAAATTGTCAGGATATGGTAATCCTTCTCGTAATGGAGGATCTAATGGAGATTTATATATTATCATTGCTATTAGACCACATAAATTTTATGAAAGATTTAACAATGATATTGTAGTTTCAATGCCTATTTCTGTTTTTTCAATTATTAATGAAGATACCATTAATGTTCCCACACCATACGGAAATAAAGATATAAAATTAAATAAAAACATGAAATTAGATGGAGTAATCACTTTAGATGGCTATGGTTTTCCTATTAAAAATAGTTCTAAAAAAGGCAACTTCATTATTACATTAAAGCCTTATATTCCTTCGCTTAACAAAGAAGATAACAAAAAAGTTCAAGAAATATTTAGCAACTATAAAGATGTCGAATTTCAAAAATGA", "aa": "MENKRDYYEVLGISKNATEKEIKSAYRKLAMQYHPDRNKAPDAEEKFKEVSEAYEVLSDPEKRQKYDKFGHSAFDQSSFGYSEDVFNSFFNSFRDMFSGGGFEVDSSDDSDDPFSSIFGFGSNRSRKTKGSDLQMKLSIDFVDSIFGKNVTVQLKKYSECSHCHGSRAESDSDIVSCPQCHGSGQVQQKMGFFSTISPCNKCGGLGKSIKNTCHVCSGKGHEYKLVNENIQIPGGINNGEYIKLSGYGNPSRNGGSNGDLYIIIAIRPHKFYERFNNDIVVSMPISVFSIINEDTINVPTPYGNKDIKLNKNMKLDGVITLDGYGFPIKNSSKKGNFIITLKPYIPSLNKEDNKKVQEIFSNYKDVEFQK", "aa_hexdigest": "393cfdc4a57ea8e0c2fc51213ca980f9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7U9", "query_cov": 1.0, "subject_cov": 0.9814323607427056, "identity": 0.995, "score": 734.0, "evalue": 2.28e-267, "valid": true, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "ec_ids": [ "1.8.4.-" ], "uniref50_id": "UniRef50_A0A448ZV77", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005515", "GO:0005524", "GO:0006457", "GO:0009408" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZV77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZV77" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "DFOKDJOJIG_442", "locus": "GCA013367855_00417" }, { "type": "cds", "sequence": "contig_1", "start": 223435, "stop": 223590, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAATTATGATTGTTGAATCACCAAATAAAGTTAATACTATTCAAAAGATAGTAGGTGATAACATAAAGGTATTGTCAAGTATTGGCCATATTTTAAAAATGTCAACATCAGGCATTGGAGGATTAGGTATAGATTTTGAAAATTGA", "aa": "MENKIMIVESPNKVNTIQKIVGDNIKVLSSIGHILKMSTSGIGGLGIDFEN", "aa_hexdigest": "5944c78f065504df67fff7c2f6f5aca3", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5484.369399999998, "isoelectric_point": 6.527242088317871 }, "id": "DFOKDJOJIG_443", "locus": "GCA013367855_00418" }, { "type": "cds", "sequence": "contig_1", "start": 223600, "stop": 224103, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF05F56" ], "nt": "ATGGTAATTGATCCAAGCAAGAGAAAAATTGTATCCGAATTAAAAGAAGCAGCTAAAAATGCTAGTGAAGTTTTAATTGCAACTGACCCCGATCGCGAAGGTGAAGCAATTGCAAATAATTTAGTAAATACTCTAAAAGTGCAAGATAAATATAAAAGGGTAAAATACAATGAAATCACTCCAGATGCCATTAATTACGCAATTGAAAACCCTTTAACAATTGATGAAAATTTAGTAAATGCTCAAAAAGCAAGAAGAATATTAGACAGGATTATTGGTTTTAAATTGTCTCAATTAATCCAAAGAAAAGTAAAAAATACTCCAACTAATCCATCTGCTGGTCGTGTTCAATCCATCGCTTTAAAACTAGTAGTTGATAAAGAAGAAGAAATTGCAAAATTTGTTCCAACATTATATTCAAAAATTGAAGCACATACTGCTGATGAACAAATTGCAAATTTCGTATATAGAGATAATTTAGATTTTGTTGGCGAAAATGAATGA", "aa": "MVIDPSKRKIVSELKEAAKNASEVLIATDPDREGEAIANNLVNTLKVQDKYKRVKYNEITPDAINYAIENPLTIDENLVNAQKARRILDRIIGFKLSQLIQRKVKNTPTNPSAGRVQSIALKLVVDKEEEIAKFVPTLYSKIEAHTADEQIANFVYRDNLDFVGENE", "aa_hexdigest": "57907904fa516acb18be52136c4d7bcf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF0FCFB", "query_cov": 0.8203592814371258, "subject_cov": 0.8726114649681529, "identity": 0.555, "score": 138.0, "evalue": 7.02e-39, "valid": false, "product": "DNA topoisomerase", "uniref50_id": "UniRef50_UPI002FF05F56" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF05F56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF05F56" ], "product": "DNA topoisomerase" }, "genes": [], "id": "DFOKDJOJIG_444", "locus": "GCA013367855_00419" }, { "type": "cds", "sequence": "contig_1", "start": 224158, "stop": 225042, "strand": "+", "frame": 1, "gene": "topA", "product": "type I DNA topoisomerase", "db_xrefs": [ "COG:COG0550", "COG:L", "EC:5.6.2.1", "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265", "KEGG:K03168", "SO:0001217", "UniRef:UniRef50_B3PMD8", "UniRef:UniRef90_D1J7V0" ], "nt": "TTGCAGGTAATAGACTTTAAAAAATCTATTCGTTCAGAGGCAATGGTTGTTCCATTTAAACAATCTATTTTGTATAAAGAATCTCAATATAGTTCGGCAACTGTTCAAATCGCTGCACAAAAACTATTTGAAATGGGGTTAATTTCTTATCCTAGAACAGATTCAACTAGACTTTCATCAATATTTATTGAAAAATCCCAAAAATTTATTGCTCAAAAATATGGAAAAGAATATATAGCAACTGAAATAAAAGGATTTTCTGGTGATCAAGATGCCCATGAAGCAATAAGACCAACAGACATTTCACTGCTTGCATCCGAAGCTCAACAAAAATTTAATTTAAGTAATGAAGAAACAAATGTATATAAATTAATCTATGAAAAGACATTAATGGCTTTGATGGCTACACCAAAACGAGAAATATATCAATACGAATTAAAAAATGGCCAATATAATTTTAATATGTCATATAGTAAAATAATTTTCGATGGATATTATAAAATTTTTGATAAAAAACAAGAATCTATTATTCCTAATTATAAAGTTGGAGATATTATTAAAGTTTTGGAATTTGTTAAATTAGACAAAGAAACTAAAGCTCCTGCAAGATATAACGATGGTTCGTTAATTAAAATGCTTGATGATATAAAAGTAGGAAGACCATCAACATTTGCAACCACAGTTAAAATTATAAAAGATAGACTATTTGTTGAAACTAAGAATAATAGTTTATATCCGACAGATTATGGGAAACTAGTTTTAGAAAAATTAATTCAAGGTTTTCCAACTACAATAAATGAAGAATATACAGCTAGCGTTGAAAACCAACTTGATTTAATTTCAGAAGGATTATCAGATTATAAAATTTTATTGAAAGATTTTTGA", "aa": "MQVIDFKKSIRSEAMVVPFKQSILYKESQYSSATVQIAAQKLFEMGLISYPRTDSTRLSSIFIEKSQKFIAQKYGKEYIATEIKGFSGDQDAHEAIRPTDISLLASEAQQKFNLSNEETNVYKLIYEKTLMALMATPKREIYQYELKNGQYNFNMSYSKIIFDGYYKIFDKKQESIIPNYKVGDIIKVLEFVKLDKETKAPARYNDGSLIKMLDDIKVGRPSTFATTVKIIKDRLFVETKNNSLYPTDYGKLVLEKLIQGFPTTINEEYTASVENQLDLISEGLSDYKILLKDF", "aa_hexdigest": "ea290b19ca87db6263ecb7a86fe10dd9", "start_type": "TTG", "rbs_motif": null, "genes": [ "topA" ], "truncated": "both", "pseudogene": { "start": 223858, "stop": 225286, "observations": { "insertion": [ 225247 ], "mutation": [ 224101, 225040 ], "stop": [ 224101, 225040 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V0", "query_cov": 1.0, "subject_cov": 0.4772727272727273, "identity": 0.99, "score": 567.0, "evalue": 4.44e-199, "gene_start": 1, "gene_end": 294, "reference_start": 242, "reference_end": 535, "reference_sequence": "MENKIMIVESPNKVNTIQKIVGDNIKVLSSIGHILKMSTSGIGGLGIDFENWEPKMVIDPSKRKIVSELKEAAKNASEVLIATDPDREGEAIANNLVNTLKVQDKYKRVKYNEITPDAINYAIENPLTIDENLVNAQKARRILDRIIGFKLSQLIQRKVKNTPTNPSAGRVQSIALKLVVDKEEEIAKFVPTLYSKIEAHTADEQIANFVYRDNLDFVGENEWIKREKVDDILAQLNQNKSLQVIDFKKSIRSEAMVVPFKQSILYKESQYSSATVQIAAQKLFEMGLISYPRTDSTRLSSIFIEKSQKFIAQKYGKEYIATEIKGFSGDQDAHEAIRPTDISLLASEAQQKFNLSNEETNVYKLIYEKTLMALMATPKREIYQYELKNGQYNFKMSYSKIIFDGYYKIFDKKQESIIPNYKVGDIIKVLEFVKLDKETKAPARYNDGSLIKMLDDIKVGRPSTFATTVKIIKDRLFVETKNNSLYPTDYGKLVLEKLIQGFPTTINEEYTASVENQLDLISEGLSDYKVLLKDFWNNFNLQLQNVNETMETDFLPQEILQEKCPLCGHDLLYRYTKSKHQKFIGCSNFPSCRYIRNIDNEKPKFLKEDIINNFYF" }, "paralog": false, "identity": 0.9894736842105263, "score": 919.0, "evalue": 0.0, "description": "Internal stop codon at 224101, 225040. Frameshift due to insertion around 225247. Nonsense mutation around 224101, 225040.", "psc": { "uniref90_id": "UniRef90_D1J7V0", "gene": "topA", "product": "type I DNA topoisomerase", "ec_ids": [ "5.6.2.1" ], "uniref50_id": "UniRef50_B3PMD8", "cog_id": "COG0550", "cog_category": "L", "kegg_orthology_id": "K03168", "go_ids": [ "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V0", "EC:5.6.2.1", "UniRef:UniRef50_B3PMD8", "COG:COG0550", "COG:L", "KEGG:K03168", "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMD8" ], "product": "DNA topoisomerase 1" } }, "id": "DFOKDJOJIG_445", "locus": "GCA013367855_00420" }, { "type": "cds", "sequence": "contig_1", "start": 225055, "stop": 225273, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01396.24" ], "nt": "TTGCAACTTCAAAATGTAAATGAAACAATGGAAACAGATTTCTTGCCTCAAGAAATTTTGCAAGAAAAATGTCCTCTTTGTGGACATGACTTGCTTTATAGATACACTAAATCAAAACATCAAAAATTTATTGGATGTTCTAACTTTCCAAGTTGTAGATATATAAGAAATATCGACAATGAAAAACCTAAATTTTTTAAAAGAAGATATAATAAATAA", "aa": "MQLQNVNETMETDFLPQEILQEKCPLCGHDLLYRYTKSKHQKFIGCSNFPSCRYIRNIDNEKPKFFKRRYNK", "aa_hexdigest": "e7c9e3d77905d61d1e4033d4c4c50f55", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01396.24", "name": "Zn_ribbon_Top1", "length": 40, "aa_cov": 0.5555555555555556, "hmm_cov": 0.95, "evalue": 1.0411185921075109e-16, "score": 56.109832763671875, "start": 21, "stop": 60 } ], "seq_stats": { "molecular_weight": 8776.033999999992, "isoelectric_point": 9.182634925842287 }, "id": "DFOKDJOJIG_446", "locus": "GCA013367855_00421" }, { "type": "cds", "sequence": "contig_1", "start": 225597, "stop": 225740, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGTTTACAATATTGATATTTATTCAAAAACTGGAAGACCAAATCCTAACGATTTAGACTTAAGAGATAAAGACAAAATTTTTAAGGGCTATAAAGATGTTACATTTGAATTCAGCACCGAAAATGTTCCGGGTAGATGA", "aa": "MNVYNIDIYSKTGRPNPNDLDLRDKDKIFKGYKDVTFEFSTENVPGR", "aa_hexdigest": "af9142e9c16b0195781ee25069e053fb", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5512.083100000001, "isoelectric_point": 6.071166038513183 }, "id": "DFOKDJOJIG_447", "locus": "GCA013367855_00422" }, { "type": "cds", "sequence": "contig_1", "start": 225864, "stop": 232376, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA13", "UniRef:UniRef90_UPI00193A1513", "PFAM:PF04778.17" ], "nt": "ATGAAAAAAAGAATAAACATTTTAATGTCAATTTCAATCGTTTGTGCTGCTGCATCAACATCAGCTATCGCTGCTATGTGCTCAATAAGCAATAACTCCACAAAAAAGTATAACGAAGCAAAATCGAGATTACTTAATCTAATTAAGAAACTAGATAATGAAGGTCAAAAGATAGCAAATGATTTTATTGCAAGACAAGATAAGAAATTTAATAGTACAGCATTTAAAAATCATTCAAACACATCAAAACTTATTGATGAAATAGAAATTTTTAGTAAAAAAATATTAGAAAATCTACAAAAAGATGAAAAACAAAGATTAGAAGAATTAAATAAATTAAATAAATTAAGATTAGATTTACAAAACTTAATTAATTCAAATGACGGTCAAAATGTCGATTCTTTTGATGCTAAAAAAGCATTAAATGAAAATCAAATAGATGATTCTTTGCCAATAGATAAAATTAAAAAGACAAATGAAAATCTTGAAAATGCAAAAAAAGAACTTCTAAATAAAATAAACGCTGAAAAAGAATTGCAATCAAAAATTTTTAATGATAAAAAACAAGAATTAAAGAGAGTGCTTGATTTAGAAGATGCAAAAGAAGTAGATTTTGCAAAAGAACAAAAAGTTTATATTGAAACAAATATAAGTGAAGCATCTTCAATTGAAGATATAAAAAACAAAATTATTGAAGTTGAAAAAGCAACTTCTTCTTTAACATCAAAAATTTTAAATACCAAACAACAAGAACTTCAAAGATTTGAAAATATTAAAAAAGACCTACAAGATTTTATTAACACAAAATTAAATGATGCAAAATATCAATCAATAAAACAAAAGGCTTTAGATAAAATAAATAGTTTAAATGGAATAAATGATGATTCAACAATTAAAGAAATAAAAGCTGGACAAAATGCTTTAATTAAAGCAAAAGAAGAAGCAGGCCGTGAAAAAGAAAAATTAGATGGTCAAAATATAAAAGATACTCTTAAAGAAACTATAAATAATGCCAAAGAATTTAAAAAGTTATTAATTGACAATGACCAAAAAATAGTTGATTTAAAATCTAATTTAGATAATGAAATTTCTAAAGCAGAACAATCTCTTTCAAAAGACAAAGAATCAATGGAATCAGCCAATGATTTATTGAATACTAAACTTATTGAATATAAAGAAATCTTAAATAAATTCAATCAAGAAAAAGAAGCTAAATTCAATGAATTAAAGCAAACAAGAAGTCAAATTGAAGCATTCATTAATGAAAATAAAACCAATCCAAATTATGCAGATTTAATTGCAAAATTAACTGATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGAAATTATTGCTGCAAATGAAGCATTACAACAAGCATTGCAAACAGCCCAATCTGCTAAAGCTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAGACCAAGAAATTCAAAAAGCAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAAGCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAGAACAAACAAGAAGTCAAATTGAAGAATTTATTGATTCAAATAAAAATAATTCAAATTATGCAGATTTGATTGAAAAATTAGCTAATGTTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCTGCAAATAATGAATTAAAACAAGCCCTTGAAAAGGCAAAAGTAGCAAAAGAACAAATTGATGATGCAAATAAATCAATTAAAGAACAATTAAATGATTCAATTGCCAATGCAAACCAATTATTAAATAAATTGGCTGATTCAGACCAACAAATTCAAAAAGCAAAAGCTGAATTAAATCAAGAAATTCAAAATGCAAATCAAGCAGTTACTTCAAACGATAATGTTTCAATGCAATCAACTAAAGAAGCATTAGATGCAAAAGTTGCTGAAATTACTAAAAAATTAGAAACATTCAATAAAGAAAAAGAAGCTAAATTTGCTGAATTAGAACAAACAAGAAGTCAAATTCAAGAATTTGTTGATTCAAATAAAAATAATTCAACTTATTCAAACATAATTTCAAAATTAACCACGACTAAAAATGAAAAAGATTCAGTTTCTGAATCTTCAAATAAATCAGATATTGAATCAGCAAACACTTTGCTAACAAATTCTTTAAAAGAAGCAAATGATAAAAAACAAGAAATCGATAAATTTGTTTCAGCTAAAAAAGAATTAGAAGAATTTATTGAAAATGATGAAGATGCTAAAAGTGATGAATTAGTTTCAAATTTAAACGATGCAAAACAAGAATTAGAAAAATTCAGTGGCATAAATGATTCATGTGAAATCACTGAAATTAAAAAAGCTACTGATGATTTAACAACTGCAAAAACAAAATTAGAAGAAGAAATTAAAAACAAAAAACAAGAGTTGTTTAATGCGTTTAAATTGTCTAAAACAACATTGCAAAACTACATTACAAACGATATTAAAGATTCAAAATATAAGATTATTCAAGATAAATTTAATAATATTATTGATGAATATAAAAACATTACATCAAACGACAAAATTTCAAAAATTAAAGATGCTACAAATAATTTAAATGCAAAATTTACAGAAATTGGAAGCAACAAGGCAATATTAGATAAGTTTTATGAATTGAAGCCAAAACTTGAAGATTTATTAAACACTGCAAATCTTAAAGATGATGCAAAATCCATAAATATTGATTTATCTTCTGAAGATCAAGCTTCTACTAATGCCAAGGCTATAAATAATAGTTCAACTATTGAAAATGTTGAACAAGTTAACCAAAAATTGCAAGCAGCAATAGATAAATTAACTAAAGATATTCAAAAAATTAATGAAGATAAAAAGGCTAAATTTGAAGAATTTAAAACAGAAAAACAACATCTAGAAGATATAATTAAAACCAAGGGTTTAGATAAATTTATTAATATCGATGATGAAATAAAATCAATAAAACAACAAATTGATGAAGAAATTAAAAAACACAAAATAAACGAAAATTCAAAAATTTCTGATATTTTAGCAGCAAAAAATAGTATCGATGAAACACAAAGAAAACTATTAGGTTCATTGGACAATTATTCAACTCAAAGAACAGAATCTATAGAAAGTTACAATAATCAAAAAACCGAATTAAGTTCTTTGATAAATTCTAATAATAATTTTAAACAATTAGAAGAATCTAAAAAAACTGAATTCAATAATAAAAAACAAGAATATGAAGACTTGAATTTAGAAACATTAAGTAAGAATAAAATTCAAGAAAAATCACAAGAAATTAAAGAATTAATAGCTAATATAAATAAAGAAATCTCAGATGCTAAAAAACAAGAATATTTGAACTTTGATAAAGCAAAAAAAGAATTAGAAAAATACATTAATGATGAATTAAATAATCAAAAATACGATTCAATTAAAAAAGAAGCACAAAATAAGATAGATAGTTTTAGTGCAATAAAAGAAGAATCTTTATCAACTAAAGAAATTCAAGAAATTAAAGATGCAACAAATGCATTAATCAAGGCTAAAACTAACGCTGAACAAGAAAAAGCAAAAATTGATGCCAAAGAACAACTAAATGCAAAAATTCAAGAAGCAGATAAACTTAAAGAAGAATTAATTGATAGCGATTCTACAATAACTACGCTAAAAACTAATTTGGAAAAAGAAATTGCTAATGCTAAAGAAGCACTTAAAAAAGATACTAAAGCAATTATTGAAGCAAAAGAACAATTAGCAAACAAAATTGATGAATTTAAATCAAGTCTTGACGAGTTTAATAAACAAAAAGAAACAAAGTTTAAAGAATTAGAAAAGACAAGGAAAGATATTAATGATTTCTTAACAGATAATGTTAAAAACAATCCAAATTATGCTGAACTTGTTAAAAAATTACAAAATTCTTTAAAAACACAAGAAAAAATTAACAAAACTTCAAATAAAGCAAATATTATTGCTGCAAATAATGAATTAACTAAGGCATTACAAGAAGCAAAAGTAGCAAAAGAACAAGCAGATAGAGCAAATGGCGAAGCTAATAATAAATTAACCACCAGTTTATCTGCTGCAAACGAATTATTAAAAAAATTAACTGATTCAGATGCTGAGATTCAAAATTATAAAAAACAACTTGAAAAAGAAATAGAAAAAGCAAATAATGCAATTACTTCAAAAAATACAAAATCATTGACTGATTCAAATAATTCATTAACAAATAAAACTAATGAAATTCAAGAAAAACTTAATAAATTCATCGAATTAAAAAATAAAGAATATGAAAAGTTAGAAAAAACAAGAAAAGACATTGATGAATTCATAAAACAAATTGAAAATGATCCTCAAACTAAAAAGAATTATCAAAATATAGTTAAAAACTTAAAAGATAAAAAAGCTGAAAAAAACTCAATAAATGAAAATTCTAATAAAAAAGACATAGTAAATGCTAATACCGAACTTCAAAATTCTTTAAATGAAGCTAAAGAACAAAAATCAGAAATTGATAAGTTTTATGCTGCTAAAAAAGATTTGGAAACATTAATATCAACACCAGATGCTAAAAAAGTTGGAACTAGTGAAACCCAAGATTTATTAAACAAAAAGAAAACAATAAATGATTCCACAGATAAAGAAACAATCAAAAATGCAACAAAAGAATTAAATGATGCCAAAACTAATTTAGAGCAAAAAATTAAAGATAAGAAAAATGAATTATTAAAAGATTTCAATAACGCAAAAGATGATTTGGAAAATTATCAAGTAAGTACTGATGTTTCTTCATTAGATAACTTTGATGAAATTAGCTTCTATAAGCCTATTCAAAAAGTTTTAGATTTATTTAATAATAATGTAAGAAAAATAGATGAAAATTCAACAATCGAAGAAATTGAAACATCAATAAAGACATTAAAAGACACAAAAGCTAATGCAATCAAGGAAGAAGAAAAATACCGCGAATTAGCAAAGTTGCATAAAACCATTGAAAAAGCTGAATCTTTTAAAAAAGAATTAGAATCAGAATTAAAAAATAATAATGATCATATACTTTCGACAATAAAGAATGATTTAAATTCAGCAATAGAAGAAGCTAAAACTATTAAATCTGATGACGCAAAAATAATTAGCGATGGAAATAATAAATTAGAATCAAAAATTTCTTATTCAAAAGAAGCATTTGAAAAATTTAAAGAATATAAAAAGTTAGAACAAACAAGAAAAGATATTGATGAATTTATAAAACAAATTGAAAATGATCATCAAACTAAAGGCAATTATCTAAATATAGTTGAAAACTTAAAAAATAAAAAAGCTGAAAAAAATTCAATAACTTTCTCTAATAATAAAAAAGAAATTCAAGATGCATATCAATCATTGCAAAATGAATTAAATAATGCAAAGATTACAAAAAAAGATATTACTGATTTCCACAACAGCAAAAATCAATTGGAAGGTTTGATAAAAACTGATGATGCTAAAAAAGTAGGAACTACAGAAGCAGATACAATTTTAACCCATTATAAAAATATATCGGATGCTTCAAAAAATGAAGAAATTAAACAAGCAACCCAAAAAATAAATGATATTAAAAAAATAATAGAAACTAAAATTCAAGAAAAGAAACGTAATGAATTTTCTAAATTTGAATCAATAAAAAATGATTTGCAAAGTTTTATTAATAAAGATTTGAAGGATCAAAAATATAATTCAATTAGAACAAAAATTGAAGATAAAATAAAAGGTGTTTCAAACATAAATAAAAATTCAAAAATTCAAGATATAGAAAATGCTAAAATTAAATTAGAATCTGCTAAAAACGAGTTTGACAGAATAATAAATAATATTAAAGAATTCAATTCCCAAAAACAAAAAGTTGAAGAAAAGTTAAACTTTTTAGATACTGAAAACATTGGCACCTCAGGTAGTCTTTCAAACAAAAATAAAAATATAAAGGCAATTTTCCAAGGTTTCATAACCGAAATGGAAAAAGAATTAATTAATAAAAATGCTGACAATAAAGCAAAAGATGAATGCATTAAGAAAATAGAATTTTTAGACAAGGCTTTAATTTCAAGATATGAAGGAATTTTGAAAGCATCATATAAATTTTTTGAAGATGCATCAACACAAAATAATCAAACAGAAAAAAATGAATTTTTTGATAATTTATGCAATGATTTAGTTGATCAAAAAGTGTTTGATCAACTAGAAAAAATGAATATGCTTCATTTTGAAGATTTTTATAATACATTCTACAACAAAGGTAATGCGCCTGAAGAAGTTAAAAAACAAAATGAATTAGAAGGGCCTGCTGTAAAGTGA", "aa": "MKKRINILMSISIVCAAASTSAIAAMCSISNNSTKKYNEAKSRLLNLIKKLDNEGQKIANDFIARQDKKFNSTAFKNHSNTSKLIDEIEIFSKKILENLQKDEKQRLEELNKLNKLRLDLQNLINSNDGQNVDSFDAKKALNENQIDDSLPIDKIKKTNENLENAKKELLNKINAEKELQSKIFNDKKQELKRVLDLEDAKEVDFAKEQKVYIETNISEASSIEDIKNKIIEVEKATSSLTSKILNTKQQELQRFENIKKDLQDFINTKLNDAKYQSIKQKALDKINSLNGINDDSTIKEIKAGQNALIKAKEEAGREKEKLDGQNIKDTLKETINNAKEFKKLLIDNDQKIVDLKSNLDNEISKAEQSLSKDKESMESANDLLNTKLIEYKEILNKFNQEKEAKFNELKQTRSQIEAFINENKTNPNYADLIAKLTDAKKAKESVSESSNKSEIIAANEALQQALQTAQSAKAEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLDQEIQKAESAIASNNTAAIQALQKPFDTKISEIQNQLTEFNKDKTNKFNELEQTRSQIEEFIDSNKNNSNYADLIEKLANVKKAKESVSESSNKSDIIAANNELKQALEKAKVAKEQIDDANKSIKEQLNDSIANANQLLNKLADSDQQIQKAKAELNQEIQNANQAVTSNDNVSMQSTKEALDAKVAEITKKLETFNKEKEAKFAELEQTRSQIQEFVDSNKNNSTYSNIISKLTTTKNEKDSVSESSNKSDIESANTLLTNSLKEANDKKQEIDKFVSAKKELEEFIENDEDAKSDELVSNLNDAKQELEKFSGINDSCEITEIKKATDDLTTAKTKLEEEIKNKKQELFNAFKLSKTTLQNYITNDIKDSKYKIIQDKFNNIIDEYKNITSNDKISKIKDATNNLNAKFTEIGSNKAILDKFYELKPKLEDLLNTANLKDDAKSINIDLSSEDQASTNAKAINNSSTIENVEQVNQKLQAAIDKLTKDIQKINEDKKAKFEEFKTEKQHLEDIIKTKGLDKFINIDDEIKSIKQQIDEEIKKHKINENSKISDILAAKNSIDETQRKLLGSLDNYSTQRTESIESYNNQKTELSSLINSNNNFKQLEESKKTEFNNKKQEYEDLNLETLSKNKIQEKSQEIKELIANINKEISDAKKQEYLNFDKAKKELEKYINDELNNQKYDSIKKEAQNKIDSFSAIKEESLSTKEIQEIKDATNALIKAKTNAEQEKAKIDAKEQLNAKIQEADKLKEELIDSDSTITTLKTNLEKEIANAKEALKKDTKAIIEAKEQLANKIDEFKSSLDEFNKQKETKFKELEKTRKDINDFLTDNVKNNPNYAELVKKLQNSLKTQEKINKTSNKANIIAANNELTKALQEAKVAKEQADRANGEANNKLTTSLSAANELLKKLTDSDAEIQNYKKQLEKEIEKANNAITSKNTKSLTDSNNSLTNKTNEIQEKLNKFIELKNKEYEKLEKTRKDIDEFIKQIENDPQTKKNYQNIVKNLKDKKAEKNSINENSNKKDIVNANTELQNSLNEAKEQKSEIDKFYAAKKDLETLISTPDAKKVGTSETQDLLNKKKTINDSTDKETIKNATKELNDAKTNLEQKIKDKKNELLKDFNNAKDDLENYQVSTDVSSLDNFDEISFYKPIQKVLDLFNNNVRKIDENSTIEEIETSIKTLKDTKANAIKEEEKYRELAKLHKTIEKAESFKKELESELKNNNDHILSTIKNDLNSAIEEAKTIKSDDAKIISDGNNKLESKISYSKEAFEKFKEYKKLEQTRKDIDEFIKQIENDHQTKGNYLNIVENLKNKKAEKNSITFSNNKKEIQDAYQSLQNELNNAKITKKDITDFHNSKNQLEGLIKTDDAKKVGTTEADTILTHYKNISDASKNEEIKQATQKINDIKKIIETKIQEKKRNEFSKFESIKNDLQSFINKDLKDQKYNSIRTKIEDKIKGVSNINKNSKIQDIENAKIKLESAKNEFDRIINNIKEFNSQKQKVEEKLNFLDTENIGTSGSLSNKNKNIKAIFQGFITEMEKELINKNADNKAKDECIKKIEFLDKALISRYEGILKASYKFFEDASTQNNQTEKNEFFDNLCNDLVDQKVFDQLEKMNMLHFEDFYNTFYNKGNAPEEVKKQNELEGPAVK", "aa_hexdigest": "e3bdd27147e25bee2933d442a6af9b67", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI00193A1513", "query_cov": 1.0064516129032257, "subject_cov": 0.9305496378355347, "identity": 0.902, "score": 3183.0, "evalue": 0.0, "valid": true, "uniref50_id": "UniRef50_A0A454CA13" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA13" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 156, "aa_cov": 0.07142857142857142, "hmm_cov": 1.0, "evalue": 5.950620240649373e-249, "score": 811.5527954101562, "start": 494, "stop": 648 } ], "seq_stats": { "molecular_weight": 247925.69120000213, "isoelectric_point": 5.90678768157959 }, "id": "DFOKDJOJIG_448", "locus": "GCA013367855_00423" }, { "type": "cds", "sequence": "contig_1", "start": 232820, "stop": 233329, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V2", "UniRef:UniRef90_D1J7V2" ], "nt": "TTGCAAATTAGTAAAAATATAAAACTTGATCCGGTTGTAATTAATTCAGCTTCTGCAAAAGAAATTTATGAAAATTTTATTCATGAAAAACAAAAAGTAGATGCGTTAAGAAATAAATATATTAATCAACACCCTGAGTTGAAACCATATTTGAATAATAATACTAGAACGGCATTTGATTTTAAATTTAAAGGTCCTAAAAAGTCTGAAATTAAAAAATTACTAAAAGATATGCCTGAAGAATTTGATGCTCATAATTTCATCTTTTCAAAAATTTTGACAAATCAAACATTTACTGAAATGAAATTCTTAAAAATGAGATTTTCTAATTTAGAATTAGACACATCTGATAAAGAATCAAATAAATTAAAGGTAACATATGAAGTTTATTTGAATTATGAATATGCAACGGGAAGCTTCGAACTTAATAACGTTAAGGGCACTTCACAATCTAAATATTATTTTAAAGGAAATCAAACTGTCAAGGTAATTAGTGATTCTGAAAAGTGA", "aa": "MQISKNIKLDPVVINSASAKEIYENFIHEKQKVDALRNKYINQHPELKPYLNNNTRTAFDFKFKGPKKSEIKKLLKDMPEEFDAHNFIFSKILTNQTFTEMKFLKMRFSNLELDTSDKESNKLKVTYEVYLNYEYATGSFELNNVKGTSQSKYYFKGNQTVKVISDSEK", "aa_hexdigest": "306a130629c31974adae0f9f7ea919ea", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 232658, "stop": 233632, "observations": { "mutation": [ 232802, 233327, 233423, 233462, 233573 ], "stop": [ 232802, 233327, 233423, 233462, 233573 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V2", "query_cov": 1.0, "subject_cov": 0.49271137026239065, "identity": 0.9940000000000001, "score": 331.0, "evalue": 3.69e-112, "gene_start": 1, "gene_end": 169, "reference_start": 55, "reference_end": 223, "reference_sequence": "MKNHSNTRRKHRSLAPLYYTLGSIAAAATIAGAIYFVVKNGRRVSKQYWSIEKFLQISKNIKLDPVVINSASAKEIYENFIHEKQKVDALRNKYINQHPELKPYLNNNTRTAFDFKFKGPKKSEIKKLLKDMPEEFDAHNFIFSKILTNQTFTEMKFLKMRFSNLELDTSDKESNKLKVTYEVYLNYEYATGSFELNNVKGTSQSKYYFKGNQTVKVISDSEKWNLNSKFANNLEKNLDDARKIIRIGIVKNDPKWTQSNEFREMIFKWFKRIFIENDAFPDTYSKDEYEIMPFNETSSGDDFVRWNPKKGLNNLTIKFFYVNKKNPDIKSPGRIKIFTILKV" }, "paralog": false, "identity": 0.9753086419753086, "score": 628.0, "evalue": 1.01e-231, "description": "Internal stop codon at 232802, 233327, 233423, 233462, 233573. Nonsense mutation around 232802, 233327, 233423, 233462, 233573.", "psc": { "uniref90_id": "UniRef90_D1J7V2", "uniref50_id": "UniRef50_D1J7V2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V2", "UniRef:UniRef50_D1J7V2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7V2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19863.4035, "isoelectric_point": 9.236852836608886 }, "id": "DFOKDJOJIG_449", "locus": "GCA013367855_00424" }, { "type": "cds", "sequence": "contig_1", "start": 233691, "stop": 234020, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_020002658.1", "SO:0001217", "UniParc:UPI00036BF488", "UniRef:UniRef100_UPI00036BF488", "UniRef:UniRef50_D1J7V3", "UniRef:UniRef90_D1J7V3" ], "nt": "ATGGAAATAATTAATAAAGATCTAGAGGCAAAATTAAAAACGTTTCAAAAGCAAAATTATAAATTGCAAAAAATTTGTCAATATTTTAAAATAGATTTAAAAAAACTTAATTTAAATGAATTTTTAGATTTTAATAAAAATAGTACAAACCCCTTGTTGGATTTAAAACAAATCAAACAAATTATTGCAGAAAATCAAGGTGATTCTAATATCCTTGAAGTTTTAAACATGATTAAAGAACTAGAAAGTATTAATAAAGAAAAAACTGCAAATGATGAAACTTATAAACAAGATCAAATTTTAAAAAAAATTTTAAGTAATTTAGAGTAA", "aa": "MEIINKDLEAKLKTFQKQNYKLQKICQYFKIDLKKLNLNEFLDFNKNSTNPLLDLKQIKQIIAENQGDSNILEVLNMIKELESINKEKTANDETYKQDQILKKILSNLE", "aa_hexdigest": "12ac9be0a0dcbb35ca4c3fce14756f9d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00036BF488", "ncbi_nrp_id": "WP_020002658.1", "uniref100_id": "UniRef100_UPI00036BF488", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036BF488", "RefSeq:WP_020002658.1", "UniRef:UniRef100_UPI00036BF488" ] }, "ips": { "uniref100_id": "UniRef100_UPI00036BF488", "uniref90_id": "UniRef90_D1J7V3" }, "psc": { "uniref90_id": "UniRef90_D1J7V3", "uniref50_id": "UniRef50_D1J7V3" }, "pscc": { "uniref50_id": "UniRef50_D1J7V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12889.783599999988, "isoelectric_point": 7.732285499572753 }, "id": "DFOKDJOJIG_450", "locus": "GCA013367855_00425" }, { "type": "cds", "sequence": "contig_1", "start": 234091, "stop": 234285, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "ATGAAAACACAAAAGCGTATTAATATTGCAATTGATGGACCTGCTGGTGTTGGCAAAACTATTATGTCAACAATGCTAGCAAAAACATTAGGTTATAAATTTTTAAGTAGTGGTAGTTTTTATAGAGTTGTTGCCTACAATGCATTACAAAATAGACTAGATTTAAATGATGAAGAATCTATTAATAATGCATGA", "aa": "MKTQKRINIAIDGPAGVGKTIMSTMLAKTLGYKFLSSGSFYRVVAYNALQNRLDLNDEESINNA", "aa_hexdigest": "9d43ae697aa19f7caf7f9de94e3a7a66", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 54, "aa_cov": 0.84375, "hmm_cov": 1.0, "evalue": 2.35493230615594e-17, "score": 59.0133171081543, "start": 9, "stop": 62 } ], "seq_stats": { "molecular_weight": 7040.021100000001, "isoelectric_point": 9.520900535583493 }, "id": "DFOKDJOJIG_451", "locus": "GCA013367855_00426" }, { "type": "cds", "sequence": "contig_1", "start": 234376, "stop": 234531, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "ATGGCCGCATCAGCAATAGCAAAATTTCCTTTAATAAGGGCAAAGGTTAATAAGTTTATTCAATCATTTGGTCTTAAATACAAAGGAATTATTGTTGATGGTAGAGATGCAACATATAGAATCTTGCCGCAGGCAGAGGTTAAATTCTTTTTGTGA", "aa": "MAASAIAKFPLIRAKVNKFIQSFGLKYKGIIVDGRDATYRILPQAEVKFFL", "aa_hexdigest": "022ecf965105dc92e068de5a1e762d7b", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 50, "aa_cov": 0.9803921568627451, "hmm_cov": 0.98, "evalue": 8.241411981269419e-12, "score": 40.899166107177734, "start": 2, "stop": 51 } ], "seq_stats": { "molecular_weight": 5745.824500000001, "isoelectric_point": 10.21090564727783 }, "id": "DFOKDJOJIG_452", "locus": "GCA013367855_00427" }, { "type": "cds", "sequence": "contig_1", "start": 234586, "stop": 234774, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "TTGAAAGCCGACTATAATGAGGTATTAGAAGATATAAAAATAAGAGATTATAATGACACAAATAGAAAATTTGATCCATTAAAAATTAGCGAAGGCAGCATTGTTATTGACACTTCAAATATGAGCATTCAAGAAAATTTTGACGTAATGTATAAAGAAGTGCGAAAGAGGCTTGATGATGAGAAATAA", "aa": "MKADYNEVLEDIKIRDYNDTNRKFDPLKISEGSIVIDTSNMSIQENFDVMYKEVRKRLDDEK", "aa_hexdigest": "6346b732c13b72ef172b74dc59ed9267", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 53, "aa_cov": 0.8548387096774194, "hmm_cov": 1.0, "evalue": 2.9525601033715027e-12, "score": 42.355751037597656, "start": 3, "stop": 55 } ], "seq_stats": { "molecular_weight": 7417.236000000002, "isoelectric_point": 4.679520225524903 }, "id": "DFOKDJOJIG_453", "locus": "GCA013367855_00428" }, { "type": "cds", "sequence": "contig_1", "start": 234764, "stop": 234907, "strand": "+", "frame": 2, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7C7AIQ0" ], "nt": "ATGAGAAATAATATTGTTGCAATAATTGGAAAACCTAATGTCGGTAAAAGTACCTTATTTAATAAAATCATAAACAAAAGAAAATCCATTGTTTATGATACGCCCGGGGTTACTAGAGATAGATTATATGAGGATGCAATTTGA", "aa": "MRNNIVAIIGKPNVGKSTLFNKIINKRKSIVYDTPGVTRDRLYEDAI", "aa_hexdigest": "62f2c355d3a368a5bd3cf56b2c965ad1", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A7C7AIQ0", "query_cov": 0.9574468085106383, "subject_cov": 0.8035714285714286, "identity": 0.711, "score": 68.2, "evalue": 2.66e-14, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A7C7AIQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A7C7AIQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7C7AIQ0" ], "product": "GTP-binding protein" }, "genes": [], "id": "DFOKDJOJIG_454", "locus": "GCA013367855_00429" }, { "type": "cds", "sequence": "contig_1", "start": 235109, "stop": 235645, "strand": "+", "frame": 2, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "SO:0001217", "UniRef:UniRef50_Q98RC1", "UniRef:UniRef90_D1J7V5" ], "nt": "TTGGCAGTAAATAAACTTGAAGGTTCAAACGTTAATTATTATGATAATTCAATTTACTCGCTTGGTTTTGATGAAATATTTCCAATTTCAGCAATTCATGGTGATGGAATTGGAAACTTATTGGATAAAATCATAGAAAAATTAAATTTTAGTGATAAAGATGTTGAATCATATTTTAAATTGGCATTGCTTGGAAAAACCAATGTTGGTAAAAGTACATTGCTTAATACTTTGGCTAATGAAGAACGTTCAATTGTTAGCAATGTCGAAGGGACAACGCGAGACTCTGTTTCATCTTTAATAAAAATTAACGGAGAAATCTTTGAGGTCGTAGATACAGCAGGTATTAAAAGAAAAAGCAAACTAACTGAAAGTGTTGAACATTATGCTTTAATGAGAGCAAATCAATCAATTGAAGATGCAAATTTATGTCTATTGGTTTTAGATGCAACTGAAGAAGTTAGCCATTTTTCTCAAAATGTAATTGGCATAGCCTATGAATTAAAAAAACCATTAATTTTGATTGTAAATAAATGA", "aa": "MAVNKLEGSNVNYYDNSIYSLGFDEIFPISAIHGDGIGNLLDKIIEKLNFSDKDVESYFKLALLGKTNVGKSTLLNTLANEERSIVSNVEGTTRDSVSSLIKINGEIFEVVDTAGIKRKSKLTESVEHYALMRANQSIEDANLCLLVLDATEEVSHFSQNVIGIAYELKKPLILIVNK", "aa_hexdigest": "277487db227f7b0e0246b913fa0dd8ad", "start_type": "TTG", "rbs_motif": null, "genes": [ "der" ], "truncated": "both", "pseudogene": { "start": 234809, "stop": 235948, "observations": { "mutation": [ 234905, 235643, 235721 ], "stop": [ 234905, 235643, 235721 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V5", "query_cov": 1.0, "subject_cov": 0.4036281179138322, "identity": 0.9940000000000001, "score": 339.0, "evalue": 8.17e-114, "gene_start": 1, "gene_end": 178, "reference_start": 117, "reference_end": 294, "reference_sequence": "MMRNNIVAIIGKPNVGKSTLFNKIINKRKSIVYDTPGVTRDRLYEDAIWTGKKFRVVDTGGITIEDEDFKKQIKLQAQIAIDEANVIVFLIDGKEPLTVEDYYVASLLRKSNKHVLLAVNKLEGSNVNYYDNSIYSLGFDEIFPISAIHGDGIGNLLDKIIEKLNFSDKDVESYFKLALLGKTNVGKSTLLNTLANEERSIVSNVEGTTRDSVSSLIKINGEIFEVVDTAGIKRKSKLTESVEHYALMRANQSIEDANLCLLVLDATEEVSHFSQNVIGIAYELKKPLILIVNKWDLIEKDTNTMEKYKKDLLKKVKFVDWSPIVFISAKDNLRINKLRDTIIQVKNNISRKINTNQLNDLMATAQMIRPASSINGRRLSITFARQIDGSIPTFLLFVNNVDLAHFTYLRYIENQIRENYDFSGTPIELVLRNKNKKEERE" }, "paralog": false, "identity": 0.9894459102902374, "score": 716.0, "evalue": 7.2e-265, "description": "Internal stop codon at 234905, 235643, 235721. Nonsense mutation around 234905, 235643, 235721.", "psc": { "uniref90_id": "UniRef90_D1J7V5", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_Q98RC1", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V5", "EC:3.6.5.-", "UniRef:UniRef50_Q98RC1", "COG:COG1160", "COG:J", "KEGG:K03977", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RC1" ], "product": "GTPase Der" } }, "id": "DFOKDJOJIG_455", "locus": "GCA013367855_00430" }, { "type": "cds", "sequence": "contig_1", "start": 235733, "stop": 236086, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14714.11" ], "nt": "GTGTTTATATCAGCCAAAGATAATTTAAGAATAAATAAACTAAGAGATACTATTATTCAAGTCAAGAATAATATTTCAAGAAAAATCAATACAAATCAATTAAATGACTTAATGGCTACTGCTCAAATGATAAGACCTGCTTCATCAATAAACGGCAGAAGATTATCCATTACATTTGCAAGACAAATAGATGGATCAATTCCAACATTTTTATTGTTTGTAAACAATGTTGATTTAGCCCATTTCACTTATCTAAGATATATTGAAAATCAAATAAGAGAAAATTATGATTTCTCTGGAACTCCTATAGAGCTAGTTTTAAGAAATAAAAACAAAAAAGAAGAGAGAGAATAA", "aa": "MFISAKDNLRINKLRDTIIQVKNNISRKINTNQLNDLMATAQMIRPASSINGRRLSITFARQIDGSIPTFLLFVNNVDLAHFTYLRYIENQIRENYDFSGTPIELVLRNKNKKEERE", "aa_hexdigest": "51debaa0839e8831ce52cb33b0871601", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14714.11", "name": "KH_dom-like", "length": 81, "aa_cov": 0.6923076923076923, "hmm_cov": 1.0, "evalue": 2.94270032698379e-28, "score": 93.66259002685547, "start": 28, "stop": 108 } ], "seq_stats": { "molecular_weight": 13671.543899999982, "isoelectric_point": 9.982042884826662 }, "id": "DFOKDJOJIG_456", "locus": "GCA013367855_00431" }, { "type": "cds", "sequence": "contig_1", "start": 236089, "stop": 236409, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02826.25", "PFAM:PF02737.24", "PFAM:PF02558.23", "PFAM:PF03446.21", "PFAM:PF02153.23", "PFAM:PF03721.20" ], "nt": "ATGGCAAATATTTCGATTTTTGGAAGTGGCGCTATGGGTACTGCCGTGGCAACTATCTTAGCAAATAATGGTCATAATATTACAATATATGGCATAGATGATAGTGAGTTAAATGATCTAAAAAATGGTAGAAATACAAAATATTTTGAAGATGTAAATTTACCAAAATTTAAAGTTACAAAAGATAAAAAACAAGCGGTTGAAGATTGCAATTATATAGTTTTTGCAATTCCAACTAAGGCTTTGCCTGAAACATATAAAGAAGTAATTAAATTAATAAAAAAACAAGTCATAATAATAAATGTATCTAAAGGCTTTTGA", "aa": "MANISIFGSGAMGTAVATILANNGHNITIYGIDDSELNDLKNGRNTKYFEDVNLPKFKVTKDKKQAVEDCNYIVFAIPTKALPETYKEVIKLIKKQVIIINVSKGF", "aa_hexdigest": "029ab543a64c457495d1fc070900fcf8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02826.25", "name": "2-Hacid_dh_C", "length": 104, "aa_cov": 0.9622641509433962, "hmm_cov": 0.875, "evalue": 2.001711500525824e-07, "score": 26.19732666015625, "start": 4, "stop": 105 }, { "id": "PF02737.24", "name": "3HCDH_N", "length": 75, "aa_cov": 0.5849056603773585, "hmm_cov": 0.9866666666666667, "evalue": 3.493937886199871e-08, "score": 29.20623207092285, "start": 4, "stop": 65 }, { "id": "PF02558.23", "name": "ApbA", "length": 99, "aa_cov": 0.9150943396226415, "hmm_cov": 0.9797979797979798, "evalue": 2.231625938892358e-09, "score": 32.73456573486328, "start": 4, "stop": 100 }, { "id": "PF03446.21", "name": "NAD_binding_2", "length": 104, "aa_cov": 0.9433962264150944, "hmm_cov": 0.8846153846153846, "evalue": 8.277300710626779e-10, "score": 34.553951263427734, "start": 4, "stop": 103 }, { "id": "PF02153.23", "name": "PDH_N", "length": 88, "aa_cov": 0.8301886792452831, "hmm_cov": 0.8977272727272727, "evalue": 1.4318467516220737e-07, "score": 26.760461807250977, "start": 16, "stop": 103 }, { "id": "PF03721.20", "name": "UDPG_MGDP_dh_N", "length": 86, "aa_cov": 0.7358490566037735, "hmm_cov": 1.0, "evalue": 9.535152656801976e-08, "score": 27.44905662536621, "start": 3, "stop": 80 } ], "seq_stats": { "molecular_weight": 11723.46249999999, "isoelectric_point": 8.990712547302245 }, "id": "DFOKDJOJIG_457", "locus": "GCA013367855_00432" }, { "type": "cds", "sequence": "contig_1", "start": 236440, "stop": 237072, "strand": "+", "frame": 1, "gene": "gpsA", "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0240", "SO:0001217", "UniRef:UniRef50_A0A2Z5IR65", "UniRef:UniRef90_D1J7V6" ], "nt": "ATGACGCAAGAAGCCCAAGCTAATTCATTAATAAGTGGTGTAGTTTCATTAATTGGGCCATCTTTTGCAATAGACATAATTAATAAAAATATAACTGCCGTTGACGCTGTTTCTAATAATGATAAACTAAATAAAAAAGTCCAAAAATTATTTTCAAATGATTATTTTAGAGTTTATACGCAAAAAGATGTGGCAGGAGCAGAATCAGGATCAATTTTTAAGAATATTTTAGCGATTGCTAGCGGTATGGTTGCTGGGCTTGGATATAGCACAAATAGCCAAATAGCCTTAATCACTCGAGGATTTAATGAACTAAAAAAATTTGTTTTAGCTCGTGGGGGTAAGCTAAAAACGATTTTTGGATTGTCGTGCCTGGGTGATTTAATGCTAACAGCATTGTCGGATAAATCACGAAATTACACATTTGGAAAAAATTTTTATAACAAAAATTTTGATAGTTCAAAATTAACAGTGGAAGGTCTTAGATCAATAGAAATTGTTTACGATGAATATATAAAAACAAAACTATTGGATTTGCCTATTGTAAATTCACTTTATCAAATAATATACAAGAATGCTAACCCTGATGAAATAATAATTAATTTAATGAAAAGAAAGCTAAAATCAGAATAA", "aa": "MTQEAQANSLISGVVSLIGPSFAIDIINKNITAVDAVSNNDKLNKKVQKLFSNDYFRVYTQKDVAGAESGSIFKNILAIASGMVAGLGYSTNSQIALITRGFNELKKFVLARGGKLKTIFGLSCLGDLMLTALSDKSRNYTFGKNFYNKNFDSSKLTVEGLRSIEIVYDEYIKTKLLDLPIVNSLYQIIYKNANPDEIIINLMKRKLKSE", "aa_hexdigest": "9fd4bbb099ef1318c01297716c2244ba", "start_type": "ATG", "rbs_motif": null, "genes": [ "gpsA" ], "truncated": "5-prime", "pseudogene": { "start": 236140, "stop": 237072, "observations": { "mutation": [ 236407 ], "stop": [ 236407 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V6", "query_cov": 1.0, "subject_cov": 0.6422018348623854, "identity": 0.99, "score": 397.0, "evalue": 1.12e-137, "gene_start": 1, "gene_end": 210, "reference_start": 118, "reference_end": 327, "reference_sequence": "MANISIFGSGAMGTAVATILANNGHNITIYGIDDSELNDLKNGRNTKYFEDVNLPKFKVTKDKKQAVEDCNYIVFAIPTKALPETYKEVIKLIKKQVIIINVSKGFWKDSNKSCHEIMTQEAQDNSLISGVVSLIGPSFAIDIINKNITAVDAVSNNDKLNKKVQKLFSNDYFRVYTQKDVAGAESGSIFKNILAIASGMVAGLGYSTNSQIALITRGFNELKKFVLARGGKLKTIFGLSCLGDLMLTALSDKSRNYTFGKNFYNKNFDSSKLTVEGLRSIEIVYDEYIKTKLLDLPIVNSLYQIIYKNANPNEIIINLMKRKLKSE" }, "paralog": false, "identity": 0.9903225806451613, "score": 598.0, "evalue": 1.49e-219, "description": "Internal stop codon at 236407. Nonsense mutation around 236407.", "psc": { "uniref90_id": "UniRef90_D1J7V6", "gene": "GpsA", "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A2Z5IR65", "cog_id": "COG0240", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V6", "UniRef:UniRef50_A0A2Z5IR65", "COG:COG0240", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z5IR65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z5IR65" ], "product": "Glycerol-3-phosphate dehydrogenase [NAD(P)+]" } }, "id": "DFOKDJOJIG_458", "locus": "GCA013367855_00433" }, { "type": "cds", "sequence": "contig_1", "start": 237087, "stop": 237368, "strand": "+", "frame": 3, "gene": "hupA", "product": "HU family DNA-binding protein", "db_xrefs": [ "COG:B", "COG:COG0776", "RefSeq:WP_036432820.1", "SO:0001217", "UniParc:UPI0005136244", "UniRef:UniRef100_A0A454C9F8", "UniRef:UniRef50_N9UB80", "UniRef:UniRef90_A0A454C9F8" ], "nt": "ATGAACAAGAAAGAACTACTAAGTAAAGCTAGCAATGAAACAGGATTTGCGCATGTATTAATCGAATCTGCATTTGATGAAATAATCAGAATAATTGCCGACGAAATAGAGGCAGGAAGAGAAGTTACAATTTCAGGATTTGGAAACTTTTCTTCTAAATTTATAAAAGGCAAGGAAAAGGTTCATAACATTACTAAAGAAATTATTAATGTTCCAGATAGATTGGATCCACGTTTTAAATTCTCGACAGCTTTTAAAAAACGTATTAATAGTAAAAATTAA", "aa": "MNKKELLSKASNETGFAHVLIESAFDEIIRIIADEIEAGREVTISGFGNFSSKFIKGKEKVHNITKEIINVPDRLDPRFKFSTAFKKRINSKN", "aa_hexdigest": "160d95dc8b03550165061197dd2e4040", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0005136244", "ncbi_nrp_id": "WP_036432820.1", "uniref100_id": "UniRef100_A0A454C9F8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005136244", "RefSeq:WP_036432820.1", "UniRef:UniRef100_A0A454C9F8" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9F8", "uniref90_id": "UniRef90_A0A454C9F8" }, "psc": { "uniref90_id": "UniRef90_A0A454C9F8", "gene": "HupA", "product": "HU family DNA-binding protein", "uniref50_id": "UniRef50_N9UB80", "cog_id": "COG0776", "cog_category": "B" }, "pscc": { "uniref50_id": "UniRef50_N9UB80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UB80" ], "product": "DNA-binding histone-like protein HU" }, "genes": [ "hupA" ], "id": "DFOKDJOJIG_459", "locus": "GCA013367855_00434" }, { "type": "cds", "sequence": "contig_1", "start": 237692, "stop": 237895, "strand": "-", "frame": 3, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "COG:COG3331", "COG:R", "EC:3.1.21.10", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_A0A077L6J4", "UniRef:UniRef90_D1J7V7" ], "nt": "ATGAAAAATAAAAATAATAGAGGCATGCTCTTAGAATCTATAATAAACAACACTAATGAGTATTATTTTAAAAATGGAATTGCTTTAATCCATAAAAAAAATTTAGATATTAAATTTAAAAATGTCAAATTAGAAAAAAATAAATTACTTTTAAACAATGCAAGCATATTTTCAAAAAGCACTGTAGATTATTATGGAGTTTGA", "aa": "MKNKNNRGMLLESIINNTNEYYFKNGIALIHKKNLDIKFKNVKLEKNKLLLNNASIFSKSTVDYYGV", "aa_hexdigest": "210d5eac37e9fcd468753e0f4ecee41b", "start_type": "ATG", "rbs_motif": null, "genes": [ "recU" ], "truncated": "5-prime", "pseudogene": { "start": 237392, "stop": 237895, "observations": { "mutation": [ 237568, 237694 ], "stop": [ 237568, 237694 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V7", "query_cov": 1.0, "subject_cov": 0.40119760479041916, "identity": 0.97, "score": 126.0, "evalue": 1.38e-35, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MKNKNNRGMLLESIINNTNEYYFKNGIALIHKKNLDIKFKNVKLEKNKLLLNDASIYSKSTVDYYGVWKGRFLAFEVKSTEQKNFEKKNIKKHQIEYLNKVLLHQGIAFWIIYFKFQNEFLLIEHANFKKCLFEEKKSYICFEALKSLGKEIKLIFPGILDFLNILK" }, "paralog": false, "identity": 0.9760479041916168, "score": 325.0, "evalue": 2.31e-116, "description": "Internal stop codon at 237568, 237694. Nonsense mutation around 237568, 237694.", "psc": { "uniref90_id": "UniRef90_D1J7V7", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_A0A077L6J4", "cog_id": "COG3331", "cog_category": "R", "kegg_orthology_id": "K03700", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V7", "EC:3.1.21.10", "UniRef:UniRef50_A0A077L6J4", "COG:COG3331", "COG:R", "KEGG:K03700" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L6J4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L6J4" ], "product": "Holliday junction resolvase RecU" } }, "id": "DFOKDJOJIG_460", "locus": "GCA013367855_00435" }, { "type": "cds", "sequence": "contig_1", "start": 237932, "stop": 238534, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "TTGAATTACAACTATAAAACAATTATGAAAGTAATAAATGATTTATATGATGAGAAAGCAGATTATTATGTCGTATATAACGACACATATGAAGCATCTAGAATAAAAGAAATGCAAGAAATTTTGGAATATTACAAAGATGATGGATTTATTAGCCAAAATGAATTTGACATTTTAAAAGAAAAATCTCAATTTATATTAAGCAAGATTATTGATATAGAAAATTTATTTTTTAGACCAGCAATGGATTGCAATATAAAAAGCTCTATTGTAAATTTAGGGCAAATAAAAAATGTTTATTCAATAAAAAAGATTGAACTTTTTGTAAATGAAAATAAATTAAATTTAAAACATAAAATTTTTCCATATCACGAACTTGATGTAAAAAATGGTGGAATGGCATTGCAAATAGCAACGCTAAGAGCTTTAAATAAAATAAGAGATAATGAATGGGCGGTCAAAGAAGCTCAATTAAAAAGATATTGCCATAACGATGTTTTGGCAATGATTATGGCATTTGACTTAGTTGAATATTTATTAAAATCAAAAAATATTTATTTTAAAAACTTCGAAACATATAAAGACTTAAAGGCGAAAAAGTAG", "aa": "MNYNYKTIMKVINDLYDEKADYYVVYNDTYEASRIKEMQEILEYYKDDGFISQNEFDILKEKSQFILSKIIDIENLFFRPAMDCNIKSSIVNLGQIKNVYSIKKIELFVNENKLNLKHKIFPYHELDVKNGGMALQIATLRALNKIRDNEWAVKEAQLKRYCHNDVLAMIMAFDLVEYLLKSKNIYFKNFETYKDLKAKK", "aa_hexdigest": "62645084722a83968ee57f38fe334fcf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 72, "aa_cov": 0.36, "hmm_cov": 0.7777777777777778, "evalue": 1.8637395562520257e-06, "score": 23.857582092285156, "start": 6, "stop": 77 } ], "seq_stats": { "molecular_weight": 23853.383700000017, "isoelectric_point": 8.272019004821779 }, "id": "DFOKDJOJIG_461", "locus": "GCA013367855_00436" }, { "type": "cds", "sequence": "contig_1", "start": 238673, "stop": 239161, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAAATGAAGATAATAAAAAAATAAAAAATGACAAAATAATTGATTATGTAAAAAATGAATTAAATTTAAATAATGCATTTAATTTTAATAGTCGTTTTGAAATTGAAGATAACGATGCAAAAAGCAAGATAAGTAAATACACTTGCAATTTTTCTGATTTCTTAAAAATTGTTGAAAACTTTGATAGTAATAAAGAAATACACGAACTAGATTGCAATATAACAATGGATGACTTAAAAGATAATTTAAATTTAGGAATCAAATTCTACCAATTATTAGTTAAAAAGGTTATTCCTAAATACATTATTTGTCAAAAGGAATTATTAAAATTAAAAGTTAAATCATTAACTGATAATTCAATTGAAACAAAAGAAATTTTAGATTTTTATGAAAACAATTTAATTTCAATTTCACCCGATATTTTAAACAAGGATAAATGCCCTGAATATTTATTATTAAAAGAATTTAATAAAAAAATAGTTTGA", "aa": "MLNEDNKKIKNDKIIDYVKNELNLNNAFNFNSRFEIEDNDAKSKISKYTCNFSDFLKIVENFDSNKEIHELDCNITMDDLKDNLNLGIKFYQLLVKKVIPKYIICQKELLKLKVKSLTDNSIETKEILDFYENNLISISPDILNKDKCPEYLLLKEFNKKIV", "aa_hexdigest": "cf5ed1a592beb9d927420c90c6cd583e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19124.85960000001, "isoelectric_point": 5.583601188659667 }, "id": "DFOKDJOJIG_462", "locus": "GCA013367855_00437" }, { "type": "cds", "sequence": "contig_1", "start": 239306, "stop": 239938, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATTTTAGTGAGGTTTACGAAAGCGACAACCAAGAAGACAATAGTTATGAAATGTTGGAATTTGCAAATGATGATATTGAAAACATTCTTGACAAAAATTTTAATCAATACTTTGAAAATAACTCTACTAAAAACAAAGACTTTTTAAAGCTAAATACTATCGAAAAAATAAAAAAAATTCAACAAGATATGCAAAATGATTCAACACTGTTTGAAAAAATTAATTCAGATGGTAAACTAGAATTATTTAACAAAATATTGGATAATTTTTTTCAATTTCAAATGGTTGATAGTGGAATAGCCCTTGTCGAGGAACAAATTATAAATTTTTTAACAAAAAATTCTTCAAATAAAGTAAATATAATTTCTAAAAAATGTTCAACCGATGAAAAAATTTTACAAACAAAAGAATTTTTAATGCAAAATTCACTAATAATAAATCCTGTTTTTGAATATAAAAAATGTATATCAAAGCCTTTTTACTACGATAGTAAAACTAAAGAAATGGGAAATATTGTATATTCATCAAAAACCAGCCTAAAAGATTTATTAAGAGCATATTATGATTATTCTGTTCTTTCATCCTCGGGAATTGAAATAAATAAAATTTTCATTGTGAAACCTTGA", "aa": "MNDFSEVYESDNQEDNSYEMLEFANDDIENILDKNFNQYFENNSTKNKDFLKLNTIEKIKKIQQDMQNDSTLFEKINSDGKLELFNKILDNFFQFQMVDSGIALVEEQIINFLTKNSSNKVNIISKKCSTDEKILQTKEFLMQNSLIINPVFEYKKCISKPFYYDSKTKEMGNIVYSSKTSLKDLLRAYYDYSVLSSSGIEINKIFIVKP", "aa_hexdigest": "5b5f22673048b968226edf24c8094dc1", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24616.573300000025, "isoelectric_point": 4.855664253234862 }, "id": "DFOKDJOJIG_463", "locus": "GCA013367855_00438" }, { "type": "cds", "sequence": "contig_1", "start": 240396, "stop": 240866, "strand": "-", "frame": 2, "gene": null, "product": "MAGa7180 family putative nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBU0", "UniRef:UniRef90_D1J7V9" ], "nt": "ATGGAAAAAAAAATAATAATTCCTAAAAGAAAATTTTTTAACAATAAAAATTACACTTTGGATTATAAAAATAAGACTTTAATAGTAGATCCAAAAATCTTAAAATCTATGCAAGAAAAAAAACCTGGTACTTTTGGAGGATTTAAAAAAATGACAGGTTCGGCTTTGGGGGCTATAATGGGATTGAGCTCATTTGACAGCCAATTTGAAGCATTTTGTAATATATGCGGTTTTAGAAAACCAATATTGAACAGGAAATATGTTGATGCAGGTATTGCATTAGAACCAAAAATAGTAAAAATTATTGAAAAAAAATTAAATTCCAAATTGCTTAGATATGAAGCCAATCAATATAATTATGATTATTTTAAAGATAACGAGCTATTTGGAGGGTTGCCTGATGGCTATAGTCCCGAAAGACATTTAATTATTGAAATTAAAACTGTCGGGGAAAAAAATTATGAAAATTGA", "aa": "MEKKIIIPKRKFFNNKNYTLDYKNKTLIVDPKILKSMQEKKPGTFGGFKKMTGSALGAIMGLSSFDSQFEAFCNICGFRKPILNRKYVDAGIALEPKIVKIIEKKLNSKLLRYEANQYNYDYFKDNELFGGLPDGYSPERHLIIEIKTVGEKNYEN", "aa_hexdigest": "389c2215c0cf927200b0bb01dcc288b6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 240093, "stop": 240866, "observations": { "mutation": [ 240143, 240182, 240239, 240398 ], "stop": [ 240143, 240182, 240239, 240398 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V9", "query_cov": 1.0, "subject_cov": 0.5531914893617021, "identity": 0.9940000000000001, "score": 316.0, "evalue": 1.69e-107, "gene_start": 1, "gene_end": 156, "reference_start": 1, "reference_end": 156, "reference_sequence": "MEKKIIIPKRTFFNNKNYTLDYKNKTLIVDPKILKSMQEKKPGTFGGFKKMTGSALGAIMGLSSFDSQFEAFCNICGFRKPILNRKYVDAGIALEPKIVKIIEKKLNSKLLRYEANQYNYDYFKDNELFGGLPDGYSPERHLIIEIKTVGEKNYENWIKYGVNPGYIKQAQLYTYLIGATSFSIVACFLKEEDYLNPNNVDITKRIIKNWTYILNNEQVQDDINYCMQWYKKYTQIGISPKWNEQIDQDLLKFLECNNQEEWKNLYLYWVDQGKAVPEYEQA" }, "paralog": false, "identity": 0.9766536964980544, "score": 507.0, "evalue": 3.01e-185, "description": "Internal stop codon at 240143, 240182, 240239, 240398. Nonsense mutation around 240143, 240182, 240239, 240398.", "psc": { "uniref90_id": "UniRef90_D1J7V9", "product": "MAGa7180 family putative nuclease", "uniref50_id": "UniRef50_A0A077LBU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V9", "UniRef:UniRef50_A0A077LBU0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077LBU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBU0" ], "product": "YqaJ viral recombinase domain-containing protein" } }, "id": "DFOKDJOJIG_464", "locus": "GCA013367855_00439" }, { "type": "cds", "sequence": "contig_1", "start": 241101, "stop": 241898, "strand": "+", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_036432828.1", "SO:0001217", "UniParc:UPI000512DE3E", "UniRef:UniRef100_UPI00241163AC", "UniRef:UniRef50_D1J7W0", "UniRef:UniRef90_D1J7W0" ], "nt": "ATGAAAAAATACACTAAAATTTTATTAACATTAGGAGCTATTTCTACTTCTATTGTTGCTGTTCCATTAGTAGCAGCAGGATGCAATGATCCAAAGAATAAGAAAAACCCAAACAATAATGATCAAAGTCAAGATAGCCAAATTGAGTTTCAAGAAGGCTTGGGGTTAAAAATTGCAAAAATTGCCAAAGATCAAGAAAATGTTAAAGTTGAAGAATTTGTAAAAGAAGCAAAGAGTGCAAAGACTCTAGATCAAATAAAAGCATTATTTACTAAATATAAAATTGCTTATGATTTAAGTGAAATTCCAGATGATGCAAAATATGAAGTTGCTCCTGGTACACACGGTCATGCCGATGAAGGACTAGTACACTTAGATATTATCCAAACAGTGAATGAAAAGCAAAATAATAATAGATTTGAAATTAAAGGTTTTAAAAAGGAAACTGTTGAAGACACAGTCCAATTGGGAGGATATAAGTTATCAACAAAAATCCTAGATAATAAAAAAGATTTTTCTTTGCAAGAAATAAAGAAACAAATTATTGACGCTCAAGAAAAAGGCTTTAATTCTTTACTTGAAAAATTAAAAGAATTTGTTAATATTGAAGTTGATGAAAATAATAAAACGAAACAATTTGAATTTAATAAAGAAAAGATTGAATTAATTAGTCAATCTGGTCAAATTGTTTTTAAAGGAATTTCTATTATTGATATTAGTAAAAAAGTAAATGAAGTCGGATATAAAACCGAAAGCAAAACTGATTTTATAATTCAAGGCTTGAACAAAGTTGAATAA", "aa": "MKKYTKILLTLGAISTSIVAVPLVAAGCNDPKNKKNPNNNDQSQDSQIEFQEGLGLKIAKIAKDQENVKVEEFVKEAKSAKTLDQIKALFTKYKIAYDLSEIPDDAKYEVAPGTHGHADEGLVHLDIIQTVNEKQNNNRFEIKGFKKETVEDTVQLGGYKLSTKILDNKKDFSLQEIKKQIIDAQEKGFNSLLEKLKEFVNIEVDENNKTKQFEFNKEKIELISQSGQIVFKGISIIDISKKVNEVGYKTESKTDFIIQGLNKVE", "aa_hexdigest": "cfacb2c8a1351ded271f3d3669dab135", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000512DE3E", "ncbi_nrp_id": "WP_036432828.1", "uniref100_id": "UniRef100_UPI00241163AC", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512DE3E", "RefSeq:WP_036432828.1", "UniRef:UniRef100_UPI00241163AC" ] }, "ips": { "uniref100_id": "UniRef100_UPI00241163AC", "uniref90_id": "UniRef90_D1J7W0" }, "psc": { "uniref90_id": "UniRef90_D1J7W0", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J7W0" }, "pscc": { "uniref50_id": "UniRef50_D1J7W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W0" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "DFOKDJOJIG_465", "locus": "GCA013367855_00440" }, { "type": "cds", "sequence": "contig_1", "start": 242530, "stop": 242877, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTATTTCACTAGGAAATTCAAAAACAAAATTATTTAAGCAAAACATTATTAAAAATTTAGTTATTGCTGAATTACAAGTCATTTTGCCTTTTTATATATTGCTTTTTAGTACTTCATTAATAATTGAGAGAATTTTTTCAATACAAGGACAAAGTATTTTTATTTCTTATGTATTTACAAAAGCAGAATTAAATTGCATAATGTTTTATTTTAGTTTTAATTTTTTTGCTTTATTGATTGCTAGATTCATAAATGAAATAATTCTAAATTATTTAAATCCTGCTCAAGCTATTGAAAAGCGTATAAGATGGATTAAAAGAAAAAGGTTAGATTATGAATGA", "aa": "MFGISLGNSKTKLFKQNIIKNLVIAELQVILPFYILLFSTSLIIERIFSIQGQSIFISYVFTKAELNCIMFYFSFNFFALLIARFINEIILNYLNPAQAIEKRIRWIKRKRLDYE", "aa_hexdigest": "2fa070c3a49f4c292525cc8ba3e36289", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13641.149499999974, "isoelectric_point": 9.809589958190916 }, "id": "DFOKDJOJIG_466", "locus": "GCA013367855_00441" }, { "type": "cds", "sequence": "contig_1", "start": 242870, "stop": 243538, "strand": "+", "frame": 2, "gene": "dppC", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG1173", "COG:EP", "SO:0001217", "UniRef:UniRef50_D1J7W2", "UniRef:UniRef90_D1J7W2" ], "nt": "ATGAATGAACTAGATAATAAAAAAGAACTTTTTCAATTTGCTAAAAAAAGACAACAAATTCACAATTACCCTATATTTCAAAGCGAAACAAAATTATTTTGGAAGAGATTCTTTAATAAAAAGAGCAACATCGTAATATTTAGTTTTTTATTAGCATTCATTTTACTAACATTAATATTACTATGTTTTGTAAAATTTTCTCCAATACATTCGATTGATAATAAAAGCGATTTTGTCAATAATTTACCTCCCTATTATTCTCAAATTATTACAAGAAATTTTGAAAGAGGATATCAACTTGATTTGATAAGATCTATTGAAAATCAAGAGTCAATTAGGGCTGCTAAATTAGGTATTAAACCAATATTTAGAATCTTGTATGATTCAGCAAGAGATAGTGGGGGAAGTTTAACTGTAAATACTGATATTGTTGCTTTAACATATAATCCCTACGATTTAATAAAGGCAATAAATTTGAATCAAAATTCAATAAATATTAATATTTTAAATAAAGTTTTACTTGGAACAACAGAAAGCGGAATTGATATTTATTCAAGGATATTTTGTGCATTTGCATTAACAACCTCAATTATATTATTGGCAATATTTATTAATATTTTTATTGGGTTTTCTCTAGCAGCATTTTATGTTTTTAATCGTTACAAATGA", "aa": "MNELDNKKELFQFAKKRQQIHNYPIFQSETKLFWKRFFNKKSNIVIFSFLLAFILLTLILLCFVKFSPIHSIDNKSDFVNNLPPYYSQIITRNFERGYQLDLIRSIENQESIRAAKLGIKPIFRILYDSARDSGGSLTVNTDIVALTYNPYDLIKAINLNQNSININILNKVLLGTTESGIDIYSRIFCAFALTTSIILLAIFINIFIGFSLAAFYVFNRYK", "aa_hexdigest": "0d3fc454d370c2f736a1855d6765b6a1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "dppC" ], "truncated": "3-prime", "pseudogene": { "start": 242870, "stop": 243841, "observations": { "mutation": [ 243536, 243596, 243632, 243668 ], "stop": [ 243536, 243596, 243632, 243668 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W2", "query_cov": 1.0, "subject_cov": 0.556390977443609, "identity": 0.995, "score": 416.0, "evalue": 3.81e-144, "gene_start": 1, "gene_end": 222, "reference_start": 1, "reference_end": 222, "reference_sequence": "MNELNNKKELFQFAKKRQQIHNYPIFQSETKLFWKRFFNKKSNIVIFSFLLAFILLTLILLCFVKFSPIHSIDNKSDFVNNLPPYYSQIITRNFERGYQLDLIRSIENQESIRAAKLGIKPIFRILYDSARDSGGSLTVNTDIVALTYNPYDLIKAINLNQNSININILNKVLLGTTESGIDIYSRIFCAFALTTSIILLAIFINIFIGFSLAAFYVFNRYKWYGIVIDKIAVTLNAIPEIIWIFLLCIFLGTNWYGILIAFILISWISFYELSKNEILNLIQKEYIKAAKIIGLNNFQITYRHLFKNVLPSLIILIADRLAINILIVASLAFLDFLTAESNLNIGTIFKEALSSIKVNPQYLIIVAVYITIFCILLKLFSIGLSMTYDPKISKIINRI" }, "paralog": false, "identity": 0.9845201238390093, "score": 612.0, "evalue": 1.12e-223, "description": "Internal stop codon at 243536, 243596, 243632, 243668. Nonsense mutation around 243536, 243596, 243632, 243668.", "psc": { "uniref90_id": "UniRef90_D1J7W2", "gene": "DppC", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_D1J7W2", "cog_id": "COG1173", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W2", "UniRef:UniRef50_D1J7W2", "COG:COG1173", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W2" ], "product": "Oligopeptide transport system permease protein" } }, "id": "DFOKDJOJIG_467", "locus": "GCA013367855_00442" }, { "type": "cds", "sequence": "contig_1", "start": 244157, "stop": 244687, "strand": "+", "frame": 2, "gene": "yqdH", "product": "Iron-containing alcohol dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG1979", "SO:0001217", "UniRef:UniRef50_H3NQ88", "UniRef:UniRef90_D1J7W3" ], "nt": "ATGTTGAATTTTATTTATAAAAACGATTGCAAAATCATATTTGGAAAAGAATCAATTCAAAATTTAGAAGAAGAATTGAAATCTTTAAAAGTAAAGAAAATTTTAATTGTTTATAGTGGAAAATATATTTTTGAACTAAAAATATATGACGAAATAATTAAAGCATGCAAAAAATTAAATATAGAATTTATTGAAAATGGTAATGTTGTTCCTAACCCAAGAATTGAATTAGTAAGAAAATTAGTATTAGAAACAAAGGCTCAAAATGTTGATTTTATACTTGCCATCGGTGGTGGTTCGGTAATTGACACCGCCAAAGCAATAGCAGTTGGAGCGAAAAGCGATGTTGATGTTTGGAAATTTTATACATATGAAGATAAACCAAATTGCGCATTACCTATCGGAGTTATTTCAACAATTGCATCTAGTGGTTCTGAATCGTCAAATTGCTCAATTTTGTCAAATGAGAATCACAAACTTGGCATAGAATATGATTTTATTATTCCAAAATTTGCAATAATTGATCCTTGA", "aa": "MLNFIYKNDCKIIFGKESIQNLEEELKSLKVKKILIVYSGKYIFELKIYDEIIKACKKLNIEFIENGNVVPNPRIELVRKLVLETKAQNVDFILAIGGGSVIDTAKAIAVGAKSDVDVWKFYTYEDKPNCALPIGVISTIASSGSESSNCSILSNENHKLGIEYDFIIPKFAIIDP", "aa_hexdigest": "e4e8c4ce9883ed67bf4548a56fa82dac", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "yqdH" ], "truncated": "3-prime", "pseudogene": { "start": 244157, "stop": 244990, "observations": { "mutation": [ 244685, 244943 ], "stop": [ 244685, 244943 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W3", "query_cov": 1.0056818181818181, "subject_cov": 0.45618556701030927, "identity": 0.983, "score": 340.0, "evalue": 4.87e-115, "gene_start": 1, "gene_end": 176, "reference_start": 1, "reference_end": 177, "reference_sequence": "MLNFIYKNDCKIIFGKDSIQNLEGELKSLKVKKILIVYSGKYIFELKIYDEIIKACKKLNIEFIENGNVVPNPRIELVRKLVLETKAQNVDFILAIGGGSVIDTAKAIAVGAKSDDVDVWKFYTYEDKPNCALPIGVISTIASSGSESSNCSILSNENHKLGIEYDFIIPKFAIIDPWYTKSLPMYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKLLMEDPNDMNARSEIFLISIFAHNNMLDSGRVADWASHRIEHELSNFYGITHGEGMAIVMVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQDEKANLLSKKLDEFYRTIKMRTRLSELGVSCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK" }, "paralog": false, "identity": 0.9784172661870504, "score": 534.0, "evalue": 9.69e-194, "description": "Internal stop codon at 244685, 244943. Nonsense mutation around 244685, 244943.", "psc": { "uniref90_id": "UniRef90_D1J7W3", "gene": "YqdH", "product": "Iron-containing alcohol dehydrogenase", "uniref50_id": "UniRef50_H3NQ88", "cog_id": "COG1979", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W3", "UniRef:UniRef50_H3NQ88", "COG:COG1979", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_H3NQ88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H3NQ88" ], "product": "Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein" } }, "id": "DFOKDJOJIG_468", "locus": "GCA013367855_00443" }, { "type": "cds", "sequence": "contig_1", "start": 244706, "stop": 244945, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCAAATTTCATGTGGCATATCTGATATTAGTTCTCATCTAATTGAAAGATATTATACAAATATAGAACATGTTGATGCAACAGATTACATGATTGAAGGACTTTTAAAGGCATTGATGATAAATGCTAAATTATTAATGGAAGATCCAAATGATATGAATGCAAGGTCAGAAGTATTTTTAATTTCTATTTTTGCACACAATAATATGTTAGATTCTGGTAGAGTTGCTGATTGA", "aa": "MYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKLLMEDPNDMNARSEVFLISIFAHNNMLDSGRVAD", "aa_hexdigest": "23b965e3c3e0931a1d406117acaf40a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8983.178399999993, "isoelectric_point": 4.593579673767089 }, "id": "DFOKDJOJIG_469", "locus": "GCA013367855_00444" }, { "type": "cds", "sequence": "contig_1", "start": 245018, "stop": 245320, "strand": "+", "frame": 2, "gene": null, "product": "iron-containing alcohol dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H1B6A3", "UniRef:UniRef90_UPI00215D79B7" ], "nt": "ATGGTTGCATATGCAAAATATATGGCAATTGAAAAACCTAAAAAATTAGCTCAATTAGCCGAAAGAGTTTTTAATGTAGACAAAGGTTTGAGCCAAGAACAAAAGGCTAATTTATTGTCAAAAAAATTAGATGAATTTTATAGAACAATAAAAATGAGAACTAGATTATCAGAATTAGGAGTAAGTTGCGAAAAGTTTCGTGAAATGGCATTAAGTGCAACTAAAAACGATAGTCAAACCATTGGTCATTATAAACCCCTTTTGTCAAAAGAAATAATTGAAATTCTAAAGATTGCAAAGTAA", "aa": "MVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQEQKANLLSKKLDEFYRTIKMRTRLSELGVSCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK", "aa_hexdigest": "e534ce28764c10ab870a1cbfeba7f7a0", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 244718, "stop": 245320, "observations": { "mutation": [ 244943 ], "stop": [ 244943 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D79B7", "query_cov": 1.0, "subject_cov": 0.425531914893617, "identity": 0.98, "score": 186.0, "evalue": 1.23e-57, "gene_start": 1, "gene_end": 100, "reference_start": 136, "reference_end": 235, "reference_sequence": "MSNENHKLGIEYDFIIPKFAIIDPWYTKSLPMYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKSLMEDPNDMNARSEIFLISIFAHNNMLDSGRVADWASHRIEHELSNFYGITHGEGMAIVMVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQEEKANLLSKKLDEFYRTIKMRTRLSELGVGCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK" }, "paralog": false, "identity": 0.975, "score": 383.0, "evalue": 9.35e-138, "description": "Internal stop codon at 244943. Nonsense mutation around 244943.", "psc": { "uniref90_id": "UniRef90_UPI00215D79B7", "product": "iron-containing alcohol dehydrogenase", "uniref50_id": "UniRef50_H1B6A3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D79B7", "UniRef:UniRef50_H1B6A3" ] }, "pscc": { "uniref50_id": "UniRef50_H1B6A3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H1B6A3" ], "product": "Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein" } }, "id": "DFOKDJOJIG_470", "locus": "GCA013367855_00445" }, { "type": "cds", "sequence": "contig_1", "start": 245372, "stop": 246559, "strand": "-", "frame": 3, "gene": "tktA", "product": "Transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J7W4", "UniRef:UniRef90_D1J7W4" ], "nt": "ATGAATTTCAAATACATTGAAGTGTCAAATGGTCAATGTCGTTCAATAATAAAAGCAATAAAAAAAGCACAAAAAATTAATCAACCTGTGTTTATTAAAATTTTAAGTCCTCAAAGCGAACAATTATTTAAAAAGAACTTTGATAATTCAATAACTCATGAAAAATTAGAAGAATTCAAAGACAAAACCTCTTTTCAAAAAACAAATTTGAGTGATTTGTATTCTGAGGTTGTTGAGTTTTATGAAAATAGATTGCAAAAAATAGAAAACAAAATAAAAACATTTAGTAATTCAAATAATTTGCAAATCTTTTTAAAAAACAATATTCGTGAAAATATTAACGATGTTGAAGAATTTAAAGAAAAAACATACATTGAAAATGCATTAAAAATAATAAATAATTTATGTGATAAATACAAAAATTTACTAGTATTATCAAATGATATTTCTTATTTTCAAAATATTAAATCGTTAAATGGGATTTTTGCAAGTAACAATCTTGGAAGAGGAATTTTCTTAGGTAATAGAGAATTTACAATGATTAATATGGCAATGGGATTAGGTTTGCATTCAAGCATAGAAACAATTGTCATTGCAGATATTAATTATTTAGGTTATTTATTGCCAACATTGCAAGTCTTAAAATATTCAAATGCAAAAATTCTTATACTATTAATCAATAATAGAAATAATTTTAGCCTAAGCAATGAGCTTAAAATATTATATTCATATGACCATATAAATTTATTGAGGCCTTGCGATTTCAATGAATTAAAAGGAGCGTTTGAATTTCATTTTAATCACACAAAAAAGTCATCAATAATTTATTTAGAAAATAAAGAAATTAAAGAAATAAAGGATACTTCAAAAGTTGAATTTGAAGATGGCCCATATTACATTAGAAAAATTAGTCAAACAACAAATATTATAGCAGCAGGATCTGATCTTAGAATAGCAGAGATTATTGCTAGAAGGTTAAATAGTTCTATTATTTCAATATCAAATATTGAAAACATTGAAAGCCTGAACTACGATAGAAATAATTCAATACTAATTGAATCTTATGAGGCATTATCTCATGAAAAAATTGCTAAATATAACATTGCATTAAATAAATTTTGCAACCTTGAAAAAAATGATGAAGAGTATATTGTAGAAAAAGTTTTAACTTCAATAGTAAAAAAATAA", "aa": "MNFKYIEVSNGQCRSIIKAIKKAQKINQPVFIKILSPQSEQLFKKNFDNSITHEKLEEFKDKTSFQKTNLSDLYSEVVEFYENRLQKIENKIKTFSNSNNLQIFLKNNIRENINDVEEFKEKTYIENALKIINNLCDKYKNLLVLSNDISYFQNIKSLNGIFASNNLGRGIFLGNREFTMINMAMGLGLHSSIETIVIADINYLGYLLPTLQVLKYSNAKILILLINNRNNFSLSNELKILYSYDHINLLRPCDFNELKGAFEFHFNHTKKSSIIYLENKEIKEIKDTSKVEFEDGPYYIRKISQTTNIIAAGSDLRIAEIIARRLNSSIISISNIENIESLNYDRNNSILIESYEALSHEKIAKYNIALNKFCNLEKNDEEYIVEKVLTSIVKK", "aa_hexdigest": "78720aa79699d3b637d8b305be16078c", "start_type": "ATG", "rbs_motif": null, "genes": [ "tktA" ], "truncated": "3-prime", "pseudogene": { "start": 245372, "stop": 246859, "observations": { "mutation": [ 246730, 246844, 246856 ], "stop": [ 246730, 246844, 246856 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W4", "query_cov": 1.0, "subject_cov": 0.6605351170568562, "identity": 0.99, "score": 738.0, "evalue": 3.24e-265, "gene_start": 1, "gene_end": 395, "reference_start": 204, "reference_end": 598, "reference_sequence": "MKNKKIDQIAIDNIKINSLNMAFSWKYSDFSSIMSGAKIFHALFGYFFNINRKNNSDVNRDRFVFFNPNILPLFYEILRLYSLISENEYLNLINNRNQNISYSNWTLDWIKSGEIFLGISGGFALGNTIAQNYLNRLDKNINHFTYWYVTERELQEGSTQEALAYAGTHNLRQLIVIYDANEIEYTVDSKSINPENIAKKVESMNFKYIEVSNGQCRSIIKAIKKAQKINQPVFIKILSPQSEQLFKKNFDNSITHEKLEEFKDKTSFQKTNLSDLYSEVVEFYENRLQKIENKIKTFSNSNNLQIFLKNNIRENINDVEEFKEKTYIENALKIINNLCDKYKNLLVLSNNISYFQNIKSLNGIFASNNLGRGIFLGNREFTMINMAMGLGLHSSIETIVIVDINYLGYLLPTLQVLKYSNAKILILLINNRNNFSLSNELKILYSYDHINLLRPCDFNELKGAFEFHFNHTKKSSIIYLENKEIKEIKDTSKVEFEDGPYYIRKISQTTNIIAAGSDLRIAELIARRLNSSIISISNIGNIESLNYDRNNSILIESYEALSHEKIAKYNIALNKFCNLEKNDEEYIVEKVLTSIVKK" }, "paralog": false, "identity": 0.9858585858585859, "score": 951.0, "evalue": 0.0, "description": "Internal stop codon at 246730, 246844, 246856. Nonsense mutation around 246730, 246844, 246856.", "psc": { "uniref90_id": "UniRef90_D1J7W4", "gene": "TktA", "product": "Transketolase", "uniref50_id": "UniRef50_D1J7W4", "cog_id": "COG0021", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W4", "UniRef:UniRef50_D1J7W4", "COG:COG0021", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W4" ], "product": "Transketolase" } }, "id": "DFOKDJOJIG_471", "locus": "GCA013367855_00446" }, { "type": "cds", "sequence": "contig_1", "start": 246854, "stop": 247069, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGGGGCAAAAATATTTCATGCTCTTTTTGGCTATTTTTTCAATATAAATAGAAAAAATAATTCTGATGTCAATAGGGATAGATTTGTTTTTTTTAATCCCAACATATTGCCACTTTTTTATGAAATTTTGAGATTATATAGTTTAATTAGTGAAAATGAATACCTTAACTTAATAAACAATAGAAATCAAAACATTTCATATTCGAATTGA", "aa": "MSGAKIFHALFGYFFNINRKNNSDVNRDRFVFFNPNILPLFYEILRLYSLISENEYLNLINNRNQNISYSN", "aa_hexdigest": "dfef931d7d257029baae6270a9bf0f01", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8528.541099999991, "isoelectric_point": 9.220993614196775 }, "id": "DFOKDJOJIG_472", "locus": "GCA013367855_00447" }, { "type": "cds", "sequence": "contig_1", "start": 247158, "stop": 247442, "strand": "-", "frame": 2, "gene": null, "product": "MMB_0454 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W5", "UniRef:UniRef90_D1J7W5" ], "nt": "ATGAACTTTCGTTATTTTGTTGAACAAGATGCAATATATGAAGAATTAAACAATATTTTTAGTTCAAATAAAAATATAAAATTAATTGGAAAAATAGAAATTAAACCAAATCGCACTAAAACAAATGTTTTTATTAATTTAAGTTACAAATTAATAAACAAAACTAGTTTTGCATATGAAACAAAAAAACTAATATTTAAGATTGAACATAAAGTTTTTTCATTAATAAATGCCAAACCAAACAATATTAATTTAATATTCCAAGGTTATGAAGATGAAAAATAA", "aa": "MNFRYFVEQDAIYEELNNIFSSNKNIKLIGKIEIKPNRTKTNVFINLSYKLINKTSFAYETKKLIFKIEHKVFSLINAKPNNINLIFQGYEDEK", "aa_hexdigest": "6260e9e8055bd6afa1fc15267aea064e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7W5", "query_cov": 1.0, "subject_cov": 0.912621359223301, "identity": 1.0, "score": 187.0, "evalue": 3.63e-60, "valid": true, "product": "MMB_0454 family protein", "uniref50_id": "UniRef50_D1J7W5" }, "pscc": { "uniref50_id": "UniRef50_D1J7W5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W5" ] }, "genes": [], "id": "DFOKDJOJIG_473", "locus": "GCA013367855_00448" }, { "type": "cds", "sequence": "contig_1", "start": 247731, "stop": 248030, "strand": "-", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_Q98Q55", "UniRef:UniRef90_D1J7W6" ], "nt": "ATGATAAATGTTAACGATTTAAAACCAGGAGTTACATTTAAATTAGAAGGCAATATTTATGTAGTGCTTGAAGCAGCACATTCAAAACAAGGCCGCGGCCAAGCAAATGTTAAAGCAAAAGTTAAAGATCTGAGAACAGGTTCGACAACAATTAAGTCATTTACTGGTGGCGAAAAAGTTGAACCAGCAATGATTGAAAAATCTAATATGGATTATCTATACAATGCAGGAGACGTTGTAGTTTTAATGGATCAAGAAACTTTTGAACAAATTGAAATACCTTCGGAACATCTTAATTGA", "aa": "MINVNDLKPGVTFKLEGNIYVVLEAAHSKQGRGQANVKAKVKDLRTGSTTIKSFTGGEKVEPAMIEKSNMDYLYNAGDVVVLMDQETFEQIEIPSEHLN", "aa_hexdigest": "4dfa5bee95f1db1ddb640fba54f22fd7", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "efp" ], "truncated": "5-prime", "pseudogene": { "start": 247473, "stop": 248030, "observations": { "mutation": [ 247733 ], "stop": [ 247733 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W6", "query_cov": 1.0, "subject_cov": 0.5351351351351351, "identity": 1.0, "score": 196.0, "evalue": 1.38e-62, "gene_start": 1, "gene_end": 99, "reference_start": 1, "reference_end": 99, "reference_sequence": "MINVNDLKPGVTFKLEGNIYVVLEAAHSKQGRGQANVKAKVKDLRTGSTTIKSFTGGEKVEPAMIEKSNMDYLYNAGDVVVLMDQETFEQIEIPSEHLNWELNFLKEGQKVIVRKFQNEVLDIELPANVNLKVVEAPDAVKGNTAQAAQKKVTLETGYVVETPLFIKEGEIVTISTETGKYTGRA" }, "paralog": false, "identity": 0.9945945945945946, "score": 358.0, "evalue": 1.19e-128, "description": "Internal stop codon at 247733. Nonsense mutation around 247733.", "psc": { "uniref90_id": "UniRef90_D1J7W6", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_Q98Q55", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W6", "UniRef:UniRef50_Q98Q55", "COG:COG0231", "COG:J", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q55" ], "product": "Elongation factor P" } }, "id": "DFOKDJOJIG_474", "locus": "GCA013367855_00449" }, { "type": "cds", "sequence": "contig_1", "start": 248289, "stop": 248927, "strand": "+", "frame": 3, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "COG:COG0171", "COG:H", "SO:0001217", "UniRef:UniRef50_A0A448ZZS2", "UniRef:UniRef90_D1J7W7" ], "nt": "TTGGGGATATCAGGTGGAATTGATAGTTCAACATTAGCGTTAATTTCTGAATTAGCTTTTCCAAATGGTTGCAAATTTTATTATTTAAAAACAGAAAATGATTCATATACAGAAAGCCATATAAAACTTTTGCAACTTAAATTAAAGACAAAAATTGAAATTATTAACTTAAGTTCTTGCTTTAAAAAAATAGTAAAGATTGCAAATATAATAAACCAAGGTTCAATTGCAAATACTAAATCCAGATTATTTATGACAACATTATATGGTCTTGCATTTCAAAACAAAAATCTTGTGCTTGGCACAGATAATTTTGATGAATATTATTTGGGATATTTTACAAAATATGGAGACGGTGGATGCGATTTACTGCCTTTTGCTAACATAAAAAAATCTGATGTATATGCTATGGCGAGTTTGTTGGGTGTTCCACAGGAAATTATTGATAAAAAACCTAGCGCAACATTATACAAAAATCAATATGATGAAGAAGAATTAGGATTTAAATACTCAGATTTTGAAAAATATCTATTAGATCCAACATCTGTTTCTGAGGAATTAATTAAAAAGATAAAGAATATTCACAAGCAAACTGAACACAAAAGAAAAGCAATCCCCAAGGGTCCAAAATTAAAATAA", "aa": "MGISGGIDSSTLALISELAFPNGCKFYYLKTENDSYTESHIKLLQLKLKTKIEIINLSSCFKKIVKIANIINQGSIANTKSRLFMTTLYGLAFQNKNLVLGTDNFDEYYLGYFTKYGDGGCDLLPFANIKKSDVYAMASLLGVPQEIIDKKPSATLYKNQYDEEELGFKYSDFEKYLLDPTSVSEELIKKIKNIHKQTEHKRKAIPKGPKLK", "aa_hexdigest": "c2cc20b24677001c3bde2f353b83fa98", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7W7", "query_cov": 1.0, "subject_cov": 0.8346456692913385, "identity": 0.991, "score": 412.0, "evalue": 7.47e-145, "valid": true, "gene": "NadE", "product": "NH(3)-dependent NAD(+) synthetase", "uniref50_id": "UniRef50_A0A448ZZS2", "cog_id": "COG0171", "cog_category": "H" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZS2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZS2" ], "product": "NH(3)-dependent NAD(+) synthetase" }, "genes": [ "nadE" ], "id": "DFOKDJOJIG_475", "locus": "GCA013367855_00450" }, { "type": "cds", "sequence": "contig_1", "start": 248927, "stop": 249109, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAATATAAAAATTCAAGCATCCTTACCTAAATGCTTGAATTTTTATATTGTTTTTTATGCTTTTAAATTGTTTGCTTTGGTTCAATTGTCAAAAAAATTATTTAAGTTTAATGTTTTTATTGTTGAAATGTTTAGTTTAATTAATCCTTTGTTTTCAATATTAAATAAGGGGTTGTAA", "aa": "MFNIKIQASLPKCLNFYIVFYAFKLFALVQLSKKLFKFNVFIVEMFSLINPLFSILNKGL", "aa_hexdigest": "4e904e27b130e7bdf3eba55b4dd31559", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7054.596299999996, "isoelectric_point": 9.924085807800292 }, "id": "DFOKDJOJIG_476", "locus": "GCA013367855_00451" }, { "type": "cds", "sequence": "contig_1", "start": 249487, "stop": 250116, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E586E53", "UniRef:UniRef90_UPI001E586E53" ], "nt": "TTGTACCAAGTAGTTGTTGAAAAAAAGGCAAATGATATCCTAGCAAAAAACTATATTTGTGAAGATGTAACTTTTAAATTATCTGCTTCAAGATCTTTAAAATCAAATACTTTAGTATCTAATACGATGCTAAAAAAATATCCAATATTTATCAAAATAAATAGAGATGAAAAAAGCAATAAATTATCAATCACTCTCTCGATTCATAGATTGATTTATGAAGTTGCTGCAAAAAAAGAAATTTCAATAAATAAAAAATCTCTAAATTTGTCGATTTATCATAAAACAAATGATGAATATATTCACTTTAAAATTGATAAATATGAAGATGGAAAACCTATATTTTTAGAAAAAAAACTGATAATAAAAATTGATGCTTATACAAAAATTAGAAATTATTTGAGCTATAAAGTCGGAGCCAATATTCTTATTAACTATGACTTAGCAAATAACCTATCAGATAATAGAAATATAGACTCAGATTTTAAAACAAGAACCCTGAAAAATATTGATATTTTTCTTACTGACAAGAGTTTTAAAGAAGATGTTAATAATTCTTCTACAACTGTAGTTAAAGAGTTTTTTGTTACATATTTTAAATTCAATCAATTGAAACAAAATATGCCTTGA", "aa": "MYQVVVEKKANDILAKNYICEDVTFKLSASRSLKSNTLVSNTMLKKYPIFIKINRDEKSNKLSITLSIHRLIYEVAAKKEISINKKSLNLSIYHKTNDEYIHFKIDKYEDGKPIFLEKKLIIKIDAYTKIRNYLSYKVGANILINYDLANNLSDNRNIDSDFKTRTLKNIDIFLTDKSFKEDVNNSSTTVVKEFFVTYFKFNQLKQNMP", "aa_hexdigest": "9403402c64373fa58b487b8839197347", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI001E586E53", "query_cov": 1.0, "subject_cov": 0.8293650793650794, "identity": 0.976, "score": 398.0, "evalue": 1.88e-139, "valid": true, "uniref50_id": "UniRef50_UPI001E586E53" }, "pscc": { "uniref50_id": "UniRef50_UPI001E586E53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E586E53" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24479.03580000002, "isoelectric_point": 9.507426643371584 }, "id": "DFOKDJOJIG_477", "locus": "GCA013367855_00452" }, { "type": "cds", "sequence": "contig_1", "start": 250470, "stop": 250733, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W9", "UniRef:UniRef90_D1J7W9" ], "nt": "ATGCGTCGTAGTAGCAACAGAGCATTCTTTTTAAAATGTTTTAATTATTTTAAGGAAATAATAAAAGAGTTAAGGGAACGAAAAATTAAAATAGATATAAATAAAATTCCTGAAATTTTTAATAAAGAGAATTTAATTTCACTTAAATTTTTAATTCAAAAGAATGTTTTAAAAACAGCAAAATTGTTTGAATCTTCATTGAGTGATGATTTGAAATGTATTTATGTTAAATATTTTAAAGAACTTAAAGATGATATTAAGTGA", "aa": "MRRSSNRAFFLKCFNYFKEIIKELRERKIKIDINKIPEIFNKENLISLKFLIQKNVLKTAKLFESSLSDDLKCIYVKYFKELKDDIK", "aa_hexdigest": "7e3466947377f46819a040f1eee5cee4", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 250386, "stop": 250733, "observations": { "mutation": [ 250403, 250472 ], "stop": [ 250403, 250472 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W9", "query_cov": 1.0, "subject_cov": 0.7565217391304347, "identity": 0.9890000000000001, "score": 169.0, "evalue": 8.3e-53, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MRRSSNRAFFLKCFNYFKEIIKELRERKIKIDINKIPEIFNKENLISLKFLIQKNVLKTAKLFESSLSDDLKCIYIKYFKELKDDIKWTDFFFSKSSYYRKLNYLILAIAWFLIF" }, "paralog": false, "identity": 0.9739130434782609, "score": 220.0, "evalue": 1.26e-75, "description": "Internal stop codon at 250403, 250472. Nonsense mutation around 250403, 250472.", "psc": { "uniref90_id": "UniRef90_D1J7W9", "uniref50_id": "UniRef50_D1J7W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W9", "UniRef:UniRef50_D1J7W9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10583.48249999999, "isoelectric_point": 9.69677028656006 }, "id": "DFOKDJOJIG_478", "locus": "GCA013367855_00453" }, { "type": "cds", "sequence": "contig_1", "start": 251075, "stop": 251254, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "TTGGATGTATTAGTTTATTCTTCAGAAAGTTTTTTACCCGGAACATCATTAAATGCAAGACTAATTGGTGCAATGAAAATGATTGATGGTGGCGAAACAGATACTAAATTAATTGCAGTTCACGCAGATGATTATAGATTAGATAATATTAAATCATTGGCAGATGTCGATCAAATGTGA", "aa": "MDVLVYSSESFLPGTSLNARLIGAMKMIDGGETDTKLIAVHADDYRLDNIKSLADVDQM", "aa_hexdigest": "2761b4dabd5cd8b4bf0800825a52388c", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 58, "aa_cov": 0.9830508474576272, "hmm_cov": 1.0, "evalue": 1.6651760139391713e-17, "score": 59.326881408691406, "start": 1, "stop": 58 } ], "seq_stats": { "molecular_weight": 6446.274400000002, "isoelectric_point": 4.331608772277833 }, "id": "DFOKDJOJIG_479", "locus": "GCA013367855_00454" }, { "type": "cds", "sequence": "contig_1", "start": 251267, "stop": 251422, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAAAAATTTAGAAGTAAACATTGAAATTAGTAAAGATTCAAAAATTAAATATGAATATGACCGCAAAAGCAACAAAATCAAAGTTGACAGAATTTTAAGAGGTGATTTTAAATATCCTGCAAATTATGGCTATTTATCTGATGCACTAGATTGA", "aa": "MKNLEVNIEISKDSKIKYEYDRKSNKIKVDRILRGDFKYPANYGYLSDALD", "aa_hexdigest": "cc1867663a18a93573bdd0ed9b325cc6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 44, "aa_cov": 0.8627450980392157, "hmm_cov": 1.0, "evalue": 2.9998993068576773e-13, "score": 45.507972717285156, "start": 7, "stop": 50 } ], "seq_stats": { "molecular_weight": 6058.826600000001, "isoelectric_point": 8.92521266937256 }, "id": "DFOKDJOJIG_480", "locus": "GCA013367855_00455" }, { "type": "cds", "sequence": "contig_1", "start": 251551, "stop": 251823, "strand": "+", "frame": 1, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "SO:0001217", "UniRef:UniRef50_B3PMK3", "UniRef:UniRef90_D1J7X1" ], "nt": "ATGAAAATATATTTATATGGAAAAATAATGCACGTAAACACTAACTATTTAATTTTAGATCATAATGGTGAGGGCGAATTGATATACGTGGCAGATATAAAAAGATTTAATAAGGATGATCTGAGAAAAATATTTATTTACGATGTGGTAAATGAGTATAAAAAAACTACTTATGGATTTGAAAATTTTAAAGAATTAATAATTTTTGAGGATTTAATTTCATTACAAGGTTTAGGACCTAAGACAGCAATTTCTCTTCTAAATTTAGGATGA", "aa": "MKIYLYGKIMHVNTNYLILDHNGEGELIYVADIKRFNKDDLRKIFIYDVVNEYKKTTYGFENFKELIIFEDLISLQGLGPKTAISLLNLG", "aa_hexdigest": "6ab85d8a3108cf0df2cf20e20a0b18c2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ruvA" ], "truncated": "3-prime", "pseudogene": { "start": 251551, "stop": 252126, "observations": { "mutation": [ 251821 ], "stop": [ 251821 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X1", "query_cov": 1.0, "subject_cov": 0.4368932038834951, "identity": 1.0, "score": 183.0, "evalue": 2.72e-57, "gene_start": 1, "gene_end": 90, "reference_start": 1, "reference_end": 90, "reference_sequence": "MKIYLYGKIMHVNTNYLILDHNGEGELIYVADIKRFNKDDLRKIFIYDVVNEYKKTTYGFENFKELIIFEDLISLQGLGPKTAISLLNLGWENLIQLIANEDKDKLCEASYVSLRIANNIIFAYKQKYAKFLSKLSDEDLLKIKSKNTSDSLREKFEYTMRMLGFKKQQISYALEKMSITENIEESVENAIKIIGMKQNESRVLDQ" }, "paralog": false, "identity": 0.9895287958115183, "score": 369.0, "evalue": 6.86e-133, "description": "Internal stop codon at 251821. Nonsense mutation around 251821.", "psc": { "uniref90_id": "UniRef90_D1J7X1", "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_B3PMK3", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X1", "EC:3.6.4.12", "UniRef:UniRef50_B3PMK3", "COG:COG0632", "COG:L", "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK3" ], "product": "Holliday junction branch migration complex subunit RuvA" } }, "id": "DFOKDJOJIG_481", "locus": "GCA013367855_00456" }, { "type": "cds", "sequence": "contig_1", "start": 251890, "stop": 252171, "strand": "+", "frame": 1, "gene": null, "product": "helix-hairpin-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BC92F", "UniRef:UniRef90_UPI00280BC92F" ], "nt": "TTGAGGATTGCAAACAATATAATTTTTGCCTATAAACAAAAATATGCAAAATTTTTATCAAAATTAAGCGATGAAGATTTACTTAAAATCAAGAGCAAAAACACAAGTGACTCTTTAAGAGAAAAATTTGAATATACAATGAGAATGCTCGGTTTTAAAAAACAACAAATTTTATACGCATTAGAAAAAATGAGTATAACCGAAAATATTGAAGAATCAGTTGAAAATGCAATAAAAATAATTGGAATGAAGCAAAATGAGTCAAGAGTTCTCGATCAATAG", "aa": "MRIANNIIFAYKQKYAKFLSKLSDEDLLKIKSKNTSDSLREKFEYTMRMLGFKKQQILYALEKMSITENIEESVENAIKIIGMKQNESRVLDQ", "aa_hexdigest": "3423d908b201400406a8a0c513859557", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 251746, "stop": 252171, "observations": { "mutation": [ 251821 ], "stop": [ 251821 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280BC92F", "query_cov": 1.0, "subject_cov": 0.6595744680851063, "identity": 0.978, "score": 169.0, "evalue": 1.2e-52, "gene_start": 1, "gene_end": 93, "reference_start": 49, "reference_end": 141, "reference_sequence": "MIIFEDLISLQGLGPKTAISLLNLGWENLIQLIANEDKDKLCEASYVSLRIANNIIFAYKQKYAKFLSKLSDEDLLKIKSKNTSDSLREKFEYTMRMLGFKKQQISYALEKMSITENIEESVENAIKIIGMKQNESRVLDQ" }, "paralog": false, "identity": 0.9787234042553191, "score": 263.0, "evalue": 7.49e-92, "description": "Internal stop codon at 251821. Nonsense mutation around 251821.", "psc": { "uniref90_id": "UniRef90_UPI00280BC92F", "product": "helix-hairpin-helix domain-containing protein", "uniref50_id": "UniRef50_UPI00280BC92F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280BC92F", "UniRef:UniRef50_UPI00280BC92F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00280BC92F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BC92F" ], "product": "helix-hairpin-helix domain-containing protein" } }, "id": "DFOKDJOJIG_482", "locus": "GCA013367855_00457" }, { "type": "cds", "sequence": "contig_1", "start": 252146, "stop": 253099, "strand": "+", "frame": 2, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.12", "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378", "RefSeq:WP_175972245.1", "SO:0001217", "UniParc:UPI00158BE11D", "UniRef:UniRef100_UPI00158BE11D", "UniRef:UniRef50_D1J7X2", "UniRef:UniRef90_D1J7X2" ], "nt": "ATGAGTCAAGAGTTCTCGATCAATAGTTTTGATGAATTTGTAGGCCAAGAAAAACTAATTTCCACTATTAAAATAATAATAGAATCTGCAAAGGTTCAAAAAAAACAAATTGATCATTTATTATTTTATGGTCCGCCAGGTTTAGGAAAAACTACACTTGCTAAAATAATTTCAAAAGAAACAAAAAGTAATATTGTTTATGTTCAAGGCCCACTTATTGAAAAAAAGAGTGATGTATTGACTATTTTTTCTTCAATAAATGATAATGACATAATTTTTATTGATGAAATTCATGGCATAAACAAAAATATTGAAGAATTATTTTATTCAGCATTAGAAGAAGGAACAATTGATGTTGCATTGGGCGTTGATGGTGATAAAAAAATCATGAGAATGAAACTAAAAAAATTTTGTTTGATTGCTGCCACAACAAAAATCAATTTATTATCCAAACCATTAAAAGATCGTTTTGGCTATATAGGAAAATTAAGCGATTATAGCGACTTAGAAATTGCTAAAATAATTTTTAACTCTGCAACTAAAAATAAGATGAAAATTAAAAATGATGCGATTAATTTTATTGCAATTCATTCAAGGCAAACACCAAGAGTCGCTAATACTCTTTTAAAAAGAGTTAATGATTTTGCCATATATTACAAAATTGATCTAATTGACTTAGAAACTGTTAAGAAAGCCTTTTCGTATTTGGGTATTTATTTGTATGGTTTGTATTCGCCTCAAATTGAATATTTAAAAACATTACATAAAGTATTTAATGACAAATTCTCTTCGCTTGATGCGATAAGTTCAATAATTAAGGATGATAAATATACTATTTTAAATGATATTGAGCCTATATTATTGATTTATAAGTTAATTGAGAAATCACCCCGTGGTAGAAAAATTACAAACAATGGCATTGAATATTTAAAGAGTCAAAATATAATTACTTAA", "aa": "MSQEFSINSFDEFVGQEKLISTIKIIIESAKVQKKQIDHLLFYGPPGLGKTTLAKIISKETKSNIVYVQGPLIEKKSDVLTIFSSINDNDIIFIDEIHGINKNIEELFYSALEEGTIDVALGVDGDKKIMRMKLKKFCLIAATTKINLLSKPLKDRFGYIGKLSDYSDLEIAKIIFNSATKNKMKIKNDAINFIAIHSRQTPRVANTLLKRVNDFAIYYKIDLIDLETVKKAFSYLGIYLYGLYSPQIEYLKTLHKVFNDKFSSLDAISSIIKDDKYTILNDIEPILLIYKLIEKSPRGRKITNNGIEYLKSQNIIT", "aa_hexdigest": "41578bc78afa84598d8676884befda1c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158BE11D", "ncbi_nrp_id": "WP_175972245.1", "uniref100_id": "UniRef100_UPI00158BE11D", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158BE11D", "RefSeq:WP_175972245.1", "UniRef:UniRef100_UPI00158BE11D" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158BE11D", "uniref90_id": "UniRef90_D1J7X2" }, "psc": { "uniref90_id": "UniRef90_D1J7X2", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_D1J7X2", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7X2" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "DFOKDJOJIG_483", "locus": "GCA013367855_00458" }, { "type": "cds", "sequence": "contig_1", "start": 253234, "stop": 253887, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAATTTTAGGATCAATTTTTTATTTGTTTCCGCAACTTAAAAAACAATCCAATTCAAATGTTACATCTGCTAAAATTGGGTTAAAAATAGAAAGGGAAAATCAAAAAGAAAACTCGTATAAAAAATCATATGGATTATCAGATCAAGAAATTTTGAATTTGACTAAGAATTACATAGGAAACCAAAACGGGGTTTTAAGTACTTCATATGATATTAATTTGGTATCTAAAAATTTGATTTCTGCTACTAGTTATCAAGATAAAACAGATAAGGATGTTAATAATTTAATATCCAATTTTGTTAATAAGCCTTATTTAACAGTAACTGATGGTGGTGGTAATCCATTATTTTATAAAGGTGAATATGTTTCTAGGTTTTATCCAAATCCTGATGTTCCTAGAACTCTTAAAGATTTTTTGAAAAATGGACCTCAAAACTACAATATTAGCGTAGAAAAAAATCCAGCAACAACCTACAACAAATTTGGTGCAAGACAAAAAATTCAAGTTAAATTGGATGAATATGGATGGGATAGTTTTGTGCAAATGATAAACGAGTACACGTTTGCAACAAATTTATCTTTACAACGTAATAGAAAAGCCTCGCGTGAAGAAATTATAAATAACCCAGGCAATAAAATTTACTTTTGA", "aa": "MTILGSIFYLFPQLKKQSNSNVTSAKIGLKIERENQKENSYKKSYGLSDQEILNLTKNYIGNQNGVLSTSYDINLVSKNLISATSYQDKTDKDVNNLISNFVNKPYLTVTDGGGNPLFYKGEYVSRFYPNPDVPRTLKDFLKNGPQNYNISVEKNPATTYNKFGARQKIQVKLDEYGWDSFVQMINEYTFATNLSLQRNRKASREEIINNPGNKIYF", "aa_hexdigest": "4e9f691035d0f36e3bd75c33ddb324ad", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24851.63180000001, "isoelectric_point": 9.381713294982909 }, "id": "DFOKDJOJIG_484", "locus": "GCA013367855_00459" }, { "type": "cds", "sequence": "contig_1", "start": 253993, "stop": 254490, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGAAATAAAAAAGGACAAAAAAGGGAATATCATTGATATTAAATATCCTGTTTTAAAATCAAAAGAAATTAATGCAAGAAAATATTTGATATCAGCAGTCAACCCCGCAACATCTAGAATACTTTCAACTGAAAAAAATGAATCGTCATTTTATTTAGCGAACGAAAATTTAGAGGGTTTAAGTGATGAATATATTGCTGCTGCAATTAACTATTCATATGCCCCATTTAAATTAACTAAAACCCACAACTATTTTTTAACCGGTAGTTCAACAAATCAAAATAGATATTTAGCAGTTTTAGCAATTCTATTTTCGTTATTTGCAGTATTTTTGATTGCTAAATATCGTTTATACGGAATTATATCTTCAATATGCTTGGCATTTATGATATTTGTGTTCTTATCAATTATTACTTCATTTAGCATATATATAACAGGAGCCGTGGCATTTACTTTCTTAGTTTCTACATTCTTAACATTTTTAATGATTTGA", "aa": "MPEIKKDKKGNIIDIKYPVLKSKEINARKYLISAVNPATSRILSTEKNESSFYLANENLEGLSDEYIAAAINYSYAPFKLTKTHNYFLTGSSTNQNRYLAVLAILFSLFAVFLIAKYRLYGIISSICLAFMIFVFLSIITSFSIYITGAVAFTFLVSTFLTFLMI", "aa_hexdigest": "dc6898de014fe195faab5db7ae2aac5a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18627.65589999999, "isoelectric_point": 9.361470222473145 }, "id": "DFOKDJOJIG_485", "locus": "GCA013367855_00460" }, { "type": "cds", "sequence": "contig_1", "start": 254803, "stop": 255000, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGCAAAAATTGATTTAATCTCAAAATCAAAATATTTTAGTATAGGTTTTGCAATATTTGTAATATTAACCATTGTATTCTTCGGAATATATAGCGGTGTATTTGGAAATACAAAATTAGGAATTAATGCTAGTGAAACATTAGCGAATAATTACTTATATTCTATTCAAAAATCATCAATAAATGGTGATTGA", "aa": "MIAKIDLISKSKYFSIGFAIFVILTIVFFGIYSGVFGNTKLGINASETLANNYLYSIQKSSINGD", "aa_hexdigest": "5dc74939cf120b0251bb17f902cf3353", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7118.2035, "isoelectric_point": 9.100760078430177 }, "id": "DFOKDJOJIG_486", "locus": "GCA013367855_00461" }, { "type": "cds", "sequence": "contig_1", "start": 255027, "stop": 255416, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATTGTCCATGGCAAATTATTAATCAAACTATTGAACTTATAACTGCTAATATTTCGTTTATTCACATTACAAAAAACGCTATTAAACCAAATCTTATAATGCTATATATTCCACAAATTATTAATGAAACCACAATTATTAAAATAGTAAATCCAAAATTGATTAATTCACCATTTGCATTTAATAATATTTCGTGTATGTTCAAATCAAATTCATTACCTATAGCTGAAATTTTAGTATTAATAAATGTCTTTAAAGCTTCTAATTTAGTTGAATCAATCTTGTTTGCTGAAGAAATTAATAAATTATATGAGTCGTTAGTTAAACTTGATTTTTCAAAGAAAATATTTGCATTTGAAATATTTAATTCTTTTCAATATTGA", "aa": "MLNCPWQIINQTIELITANISFIHITKNAIKPNLIMLYIPQIINETTIIKIVNPKLINSPFAFNNISCMFKSNSLPIAEILVLINVFKASNLVESILFAEEINKLYESLVKLDFSKKIFAFEIFNSFQY", "aa_hexdigest": "d9be451f82404b1c90865d4412af1a42", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14842.463099999983, "isoelectric_point": 7.7402429580688485 }, "id": "DFOKDJOJIG_487", "locus": "GCA013367855_00462" }, { "type": "cds", "sequence": "contig_1", "start": 255433, "stop": 255738, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTATCTACTGCATTTAGTGTAGTTGATTCTATCTCTAATTCTCAAACAATTAAGGAAGAATTTGGCAATAATTTAAATACAAAAAATTACATATTTGAAATTGGCCAAATAAACAATATATTTAAAAAATATGTAATAGAATCTTTGAATAAAAACATCTTCAATATATTCTTTGCAATTATTTTTGTGGCATCTACATATACGTTGATGACAGGCTTTAATGCAACAACAATATTAGTAACTTCAATGTTTATTATTTCAAATTCATTTATCAATTTATTTATATTACCAAACATTTGA", "aa": "MILSTAFSVVDSISNSQTIKEEFGNNLNTKNYIFEIGQINNIFKKYVIESLNKNIFNIFFAIIFVASTYTLMTGFNATTILVTSMFIISNSFINLFILPNI", "aa_hexdigest": "0b3ff594ea1627c9664994c2abbcf03e", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11451.191999999983, "isoelectric_point": 6.0701997756958 }, "id": "DFOKDJOJIG_488", "locus": "GCA013367855_00463" }, { "type": "cds", "sequence": "contig_1", "start": 255870, "stop": 257147, "strand": "+", "frame": 3, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "COG:COG0124", "COG:J", "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "KEGG:K01892", "RefSeq:WP_044420543.1", "SO:0001217", "UniParc:UPI0005C95AF8", "UniRef:UniRef100_UPI0005C95AF8", "UniRef:UniRef50_A0A3Q9VA72", "UniRef:UniRef90_D1J7X4" ], "nt": "ATGTTTCAAAGACTTAAAGGTACAAGAGATATATATGGGTTAGAAGAAAAGGTTTTGAATTTGATAGAAAATAAATTTTTTAAAATTATAAAAAATTACAATTTTAAAAGAATTTGCACACCGATAATTGAAGACGCTAACTTGTTTATAAGATCAGTTGGTGAAACTAGTGACATTGTTTCAAAAGAGATGTATCTGTTTAAGGATAATGGCCAAAGAGACATTGCCTTAAGACCAGAAGGAACCGCTTCAACAATAAGAGCTTTTGTAGAAAACAAAATAAATAATTTAGAACCTACTAAATTAGCCTATTTTGGACCTATGTTTAGATACGAACGTCCTCAAAAAGGTCGCTATAGACAATTTATTCAGGGAGGAGTTGAATTAATTGAAAATTCAACTGTTGAGAATAATTTTGAAATAATAAAATTAGCATATGATTTTTTAAAGTCATTAAAAATTAGTAACATTACTTTAGAAATAAACAATCTAGGCTCTTTTGAGTCAAGAAATAACTACATAGAAATATTAAAAGAATATTTTTCTAAATACAAAGAACAATTGAATGAAATTTCTTTAATTAGATTGCAAAAGAATGTTTTAAGAATCTTAGATGATAAAGAAGAAATAAAAAAAGATTTTGTAAAAAATGCCCCAAAATTAATAGATTATTTATCTTCAATTGAAAAAGAAAGATTTAACAATTTACTAGATTTACTAGACGAATTCGAAATCAAATATGTAATAAATCCTTACTTAGTTAGGGGGCTTGATTACTACAATGATGTTGTTTTTGAATTTGTTTCAAATTCAGATGCCCTAGGTTCACAATGTACCGTTCTTGGGGGTGGAAGATATGATGGTATGATTAAAAGCTTTGGAGGGCCTGATATAGGCTCTATTGGGTTTGCCTTTGGAGTAGATAGATTAATGGAAATAATAATGTCAAATATTGACAATTATCCAGAGCTAAGTTTTAATGAAACGATATTAATTGCTTATTTGAATGAAGATGAAAAAAATGAAATAAGAAAAATTGCTTATGATTTGAGAAGGGATTTTCAAGTAATTTTTCCAAGTCAAAGACAATCAATTAAGTCTCTGTTTAGACTTCAATATAAACTACAACCAAAATATTTAATTTTTAAAGAATTAAATATGAAAAAAAATGAGTTGAAAATAAAATCAGTTTTAAATGAAAGAAATATAATTTATACTAATTTAGACGAATTTAAAAATATTATTAGTGAAATGAAAAGAGATGAAAACAATGAATAA", "aa": "MFQRLKGTRDIYGLEEKVLNLIENKFFKIIKNYNFKRICTPIIEDANLFIRSVGETSDIVSKEMYLFKDNGQRDIALRPEGTASTIRAFVENKINNLEPTKLAYFGPMFRYERPQKGRYRQFIQGGVELIENSTVENNFEIIKLAYDFLKSLKISNITLEINNLGSFESRNNYIEILKEYFSKYKEQLNEISLIRLQKNVLRILDDKEEIKKDFVKNAPKLIDYLSSIEKERFNNLLDLLDEFEIKYVINPYLVRGLDYYNDVVFEFVSNSDALGSQCTVLGGGRYDGMIKSFGGPDIGSIGFAFGVDRLMEIIMSNIDNYPELSFNETILIAYLNEDEKNEIRKIAYDLRRDFQVIFPSQRQSIKSLFRLQYKLQPKYLIFKELNMKKNELKIKSVLNERNIIYTNLDEFKNIISEMKRDENNE", "aa_hexdigest": "3d88f8e0f5001f9db5c857baba639626", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0005C95AF8", "ncbi_nrp_id": "WP_044420543.1", "uniref100_id": "UniRef100_UPI0005C95AF8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C95AF8", "RefSeq:WP_044420543.1", "UniRef:UniRef100_UPI0005C95AF8" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C95AF8", "uniref90_id": "UniRef90_D1J7X4" }, "psc": { "uniref90_id": "UniRef90_D1J7X4", "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_A0A3Q9VA72", "cog_id": "COG0124", "cog_category": "J", "kegg_orthology_id": "K01892", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3Q9VA72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3Q9VA72" ], "product": "Histidine--tRNA ligase" }, "genes": [ "hisS" ], "id": "DFOKDJOJIG_489", "locus": "GCA013367855_00464" }, { "type": "cds", "sequence": "contig_1", "start": 257272, "stop": 257883, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "GTGCAATTAATATTTCAAAATGTATCAGATCCTTTATTAACTAAAGAAAGTTGTATTTATGTAAAAGGAACTGTTCAAAAGCGTTTAGAAGTTAATCCAGCATTAAAAACCGGAGAAATCGAAGTTATAGTTTCTGATTATAAAATTTTTAATTCAGCAAAGCAAATACCTTTTGAAATTAACAATTCAAAATCAACAAATGATGCAAACGAAGATATAAGATTGGAATATCGTTTTCTTGATTTAAGAGAAGAAAGAATGCTTAATAATTTAAGATTAAGACATAAATTCTTACTTTTGGTAAGAAATTTCTTCGATAAACAAGGTTACATTGAAGTAGAAACTCCAATTTTAGGTAAATCAACTCCTGAGGGAGCGAGAGATTACTTGGTTCCTACTAGAAGAAAAGGAAGATTTTTTGCATTGCCTCAATCACCACAACTATTTAAACAATTATTGATGGCTGCCGGATTTGAAAAATATTTCCAAATTGCCAGGGTTTTCAGAGATGAAGATTTAAGAAAAGATAGGCAACCAGAATTTACTCAATTAGATATTGAAATGTCTTTTGGAAGCCAAGAAGAAATATTTAAATTATGTGAAGAAATGTGA", "aa": "MQLIFQNVSDPLLTKESCIYVKGTVQKRLEVNPALKTGEIEVIVSDYKIFNSAKQIPFEINNSKSTNDANEDIRLEYRFLDLREERMLNNLRLRHKFLLLVRNFFDKQGYIEVETPILGKSTPEGARDYLVPTRRKGRFFALPQSPQLFKQLLMAAGFEKYFQIARVFRDEDLRKDRQPEFTQLDIEMSFGSQEEIFKLCEEM", "aa_hexdigest": "56df0164e538cf164b07c9a2d475a141", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_G9YIR7", "query_cov": 0.9310344827586207, "subject_cov": 0.8217391304347826, "identity": 0.556, "score": 193.0, "evalue": 7.66e-59, "valid": false, "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.-" ], "uniref50_id": "UniRef50_A0A951N4R3", "go_ids": [ "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 136, "aa_cov": 0.6600985221674877, "hmm_cov": 1.0, "evalue": 1.545052136414367e-60, "score": 200.42628479003906, "start": 70, "stop": 203 } ], "seq_stats": { "molecular_weight": 23943.272099999984, "isoelectric_point": 6.65228786468506 }, "id": "DFOKDJOJIG_490", "locus": "GCA013367855_00465" }, { "type": "cds", "sequence": "contig_1", "start": 257902, "stop": 258222, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGGTACAAAATTGAAACTCCGTTTCCAAAAATGGACTTTTTCTATTCGATGGCTCATTATGGAAATGATAAACCAGATACAAGATTTGGATTTTTAATCGAAGATTATTCAAAACAATTAGCAACTAAATATAATAAAAAATTTGTAAAAGCTATTGTTTTTGACAGAAATATAAATGAACATAAACAAATAATTAATGAAACTTTTAAAAAGAATAATGGACAAATATTGGATATATTTGATTTAAAAGAAGCAAACAAAAATGAATTCCAATGTCTTTTAGATAAGGCAAAAGAAGATAATATTTCAACCCCATGA", "aa": "MGYKIETPFPKMDFFYSMAHYGNDKPDTRFGFLIEDYSKQLATKYNKKFVKAIVFDRNINEHKQIINETFKKNNGQILDIFDLKEANKNEFQCLLDKAKEDNISTP", "aa_hexdigest": "c70e994752e3ded1b67ee581a190c939", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12539.144999999984, "isoelectric_point": 7.782381629943847 }, "id": "DFOKDJOJIG_491", "locus": "GCA013367855_00466" }, { "type": "cds", "sequence": "contig_1", "start": 258355, "stop": 258825, "strand": "+", "frame": 1, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FB0CEA" ], "nt": "ATGTTTGAATATGACAGAGAAACAAAAACATATTCACCTGCTCACCATGCGTTTACACAATTTGAAGAATCTACAATGAAATATTTAGAAACTGGAGAGCTTGAAAAAGTTAGAGCTAAATCATACGATTTAGTGTTAAACGGATTTGAATTAGGATCTGGTTCTATAAGAATTTATGATCCAAAGATTCAAAAGAAAATGTTCGACGTAATTAATCTAAGTGAAAAACAACAACAAGATCGTTTCGGATTCTTTTTAAAAGCATTTGAATATGGTCTACCTCCTCACAATGGTATTGCATTTGGAGTTGAAAGAATTTTAATGATTTTAACCAAATCAGAATCAATTAGAGATGTAATTGCATTTCCTAAAAATGCTAAAGGTATTGACTTGTTGACATCAGCTCCTAGCGATGTGACAAAAGAACAATTAGATGAATATGGATTGGAACTTAAAATTAATAAAAAATAA", "aa": "MFEYDRETKTYSPAHHAFTQFEESTMKYLETGELEKVRAKSYDLVLNGFELGSGSIRIYDPKIQKKMFDVINLSEKQQQDRFGFFLKAFEYGLPPHNGIAFGVERILMILTKSESIRDVIAFPKNAKGIDLLTSAPSDVTKEQLDEYGLELKINKK", "aa_hexdigest": "f007a3977b2685b4435b15c340846545", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021FB0CEA", "query_cov": 0.9743589743589743, "subject_cov": 0.8837209302325582, "identity": 0.5660000000000001, "score": 176.0, "evalue": 1.44e-53, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI0021FB0CEA" }, "pscc": { "uniref50_id": "UniRef50_UPI0021FB0CEA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FB0CEA" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "DFOKDJOJIG_492", "locus": "GCA013367855_00467" }, { "type": "cds", "sequence": "contig_1", "start": 258917, "stop": 259096, "strand": "+", "frame": 2, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0015136", "GO:0015739", "GO:0016020", "SO:0001217", "UniRef:UniRef50_I5D5Q9", "UniRef:UniRef90_D1J7X6" ], "nt": "ATGCATGCTGCTTCAATAGCCTTAACAGTTATATTGGTAATAATTTCATTTGCGATAATTGGAATTTCATTTATTATGGCTCCTGATTCAAATAGTTTTAGCGGAGCTTTAGTTGGTTCAAATGACCTAGACTTGTTCAAAGTATCAAAAGAAAGAGGAATTAAAAAAGTATTAAAGTGA", "aa": "MHAASIALTVILVIISFAIIGISFIMAPDSNSFSGALVGSNDLDLFKVSKERGIKKVLK", "aa_hexdigest": "287b3e133ec6143731f4c8470f1a19f0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "secG" ], "truncated": "3-prime", "pseudogene": { "start": 258917, "stop": 259168, "observations": { "mutation": [ 259094 ], "stop": [ 259094 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X6", "query_cov": 1.0, "subject_cov": 0.7108433734939759, "identity": 1.0, "score": 105.0, "evalue": 2.03e-28, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MHAASIALTVILVIISFAIIGISFIMAPDSNSFSGALVGSNDLDLFKVSKERGIKKVLKWSMITLGILLFIFLIALRVVIQNG" }, "paralog": false, "identity": 0.9879518072289156, "score": 151.0, "evalue": 3.2e-49, "description": "Internal stop codon at 259094. Nonsense mutation around 259094.", "psc": { "uniref90_id": "UniRef90_D1J7X6", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_I5D5Q9", "go_ids": [ "GO:0015136", "GO:0015739", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X6", "UniRef:UniRef50_I5D5Q9", "GO:0015136", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_I5D5Q9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D5Q9" ], "product": "Protein-export membrane protein SecG" } }, "id": "DFOKDJOJIG_493", "locus": "GCA013367855_00468" }, { "type": "cds", "sequence": "contig_1", "start": 259184, "stop": 260074, "strand": "+", "frame": 2, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_B3PMK9", "UniRef:UniRef90_D1J7X7" ], "nt": "ATGACATATATAAAAAATTTTAAAAATAAGAATAACAACCCTAAAATTAATGAAGAACAAGTATTAAAATGCATAAGAGAAAGTGATGGAATTACTTTTATTGAAATTGCAAAAAAATTAAGAATTCCTGCTTTTTTGAATTTTGAATTATCAGAAATATTAAATAACTATTTATCTAAAAAATTAATTGATACGAAAAGCGATGAAAAATACATCGCTATTTATTTTCTAACTAAAGTACAAAAGGAAATATCAATAACTGCCAAAAGATTAGGATTTATTGATTTTGAAATGCAAGAAGAATCTCAAACTAAAGTATTTAGTGCCTTTCTTGCACCTTTTCAATTAAAAGCGGTATTAGACAAGGATTTATTGCTTGCAAATATTTATTATTATTATGAAAATGGCATAAAAAAATATAAAGGCGATATTATTCAAATTTTAAATCACGTAAGAAAAACTATTGTTGGGACTATTGAAAAGAGCGCAAATAAACTGTTTTTTAATGCTTATGATGAAAAAGATAGGGCTAAATTTGAAATTATTAATGAAAAAGAATGCCCAGATATAACAAAATTGAAAAATTCATTGGTATCTTGTGAGATTTTAAAACCAAATAATAAAAAAGTTGCTATTGCATATAAAAATATAATTGGTAATATTAATGATAAAGAACATGTGATTAAAAAAATCATTGCCCAAAATGATGTAAATGTCAATTTTCCACAAAATTTATTAGAATATGTTTCCTCAATACCTTCTTTTGTTTCACAAGAAGAAATTAATTCAAGAATAAATTTAATAGATTTAATGACAGTTACAATTGACGGTCTTGACACTAAAGATTTTGATGACGCCATTTCGTGTTATAAAAATGAAAAGAATAATTGA", "aa": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHVRKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNN", "aa_hexdigest": "e2ec6fd4fb164e7bf6ac32ee8b533019", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "3-prime", "pseudogene": { "start": 259184, "stop": 260377, "observations": { "mutation": [ 260072 ], "stop": [ 260072 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X7", "query_cov": 1.0, "subject_cov": 0.41283124128312415, "identity": 0.997, "score": 569.0, "evalue": 1.65e-198, "gene_start": 1, "gene_end": 296, "reference_start": 1, "reference_end": 296, "reference_sequence": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNNWKLFIHIADVSYYVKENDLIDNEALLRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA" }, "paralog": false, "identity": 0.9949622166246851, "score": 780.0, "evalue": 6.76e-284, "description": "Internal stop codon at 260072. Nonsense mutation around 260072.", "psc": { "uniref90_id": "UniRef90_D1J7X7", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_B3PMK9", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X7", "EC:3.1.13.1", "UniRef:UniRef50_B3PMK9", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK9" ], "product": "Ribonuclease R" } }, "id": "DFOKDJOJIG_494", "locus": "GCA013367855_00469" }, { "type": "cds", "sequence": "contig_1", "start": 260147, "stop": 261337, "strand": "+", "frame": 2, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_B3PMK9", "UniRef:UniRef90_D1J7X7" ], "nt": "TTGCGTGGCACAAGCATTTACTTACCCGATAGGGTTATACCAATGTTGCCGGTTGAATTGTCTAACGGAATTTGTTCATTAAACCCAAATGAAGTCAGAGCTTGTATAACATTGGAATTAGAAATAGACAAGCAAGGGAATAATATATCATATAAAATTTATCCTAGTGTTATAAAATCAAATTATAGATTGACTTATAATGAAGTTAATGATTTTTATAGCAATATAAAAAGTGTTCCTGATGATGTTGCAAATATGCTGTTGCATGCACGAGATCTTGCAAAAATTATTAGAAGTAAAAAAATATCCGAAGGTTATGTTGACTTTGAAATTCAAGAATCAAAAGTTATAATGGATGGAACAAAGGTTGTTGATATCAAGGTAAAAGAAGAAGGCGAAAGCGAAAAATTAATTGAAGACTTCATGGTTCGGGCAAATGAAACCGTTGCTCAAATGATGACTAATTTAAAAATTCCTTCAATTTATAGAATTCACGAAAAACCTTCACCTGAAAAACTAACTTTATTGCAAGAATTATTCAATTTTTGTGGATTTAAGGATGTAAAAGTTCCTTATGATGGAGAACCAAAATCATTTGGAGAAATGATTGCAAAATTAAAAGAACATAAATTTGATGATTTTATTAAAATGGCATTATTAAGAACAATGCAAAAAGCAATATATTCATCAAATAATATCGGACATTTCGGTTTAGCTTCAAAAGCTTACTCACATTTTACTAGTCCAATTAGAAGATATCCAGACTTATTGCTACATAGATTGATAAGAACTTATATTTTTGAAAAGAAAGAAATAACACCCGATTTATCAGAAACGTTGAAACAAAAAATAAGTCAAATTGCTTTAATGAATAGTGAATCTGAAAAAAATGCAATGGTTGTTGAACGAAGTGTTGTTGATGTTAGAAAATCGGAATTCTTTGAAAATTTAATTAATAAGCAATTTTTTGCAACATTAGTATCAATAGAAAAATTTGGAATGTTTTTTAATATTGATGAATATCAAACAAGTGTTTTAGTTCGTTTTGAAAATCTTGCTATTCCAACTAATAAAGACAACGATTTTCAAGCATCATCTAAAAATAAAATCTATAAAGCAGGCAATAAGTATTTAATTACTATCACTTCGATAGATCATGAAAAAGGAAATGTAAATGCAACATTAGCTTAG", "aa": "MRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA", "aa_hexdigest": "52a4d609eb5fb8047bbdc1b03fd17a15", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "genes": [ "rnr" ], "truncated": "5-prime", "pseudogene": { "start": 259847, "stop": 261337, "observations": { "mutation": [ 260072 ], "stop": [ 260072 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X7", "query_cov": 1.0, "subject_cov": 0.5523012552301255, "identity": 0.997, "score": 768.0, "evalue": 3.19e-275, "gene_start": 1, "gene_end": 396, "reference_start": 322, "reference_end": 717, "reference_sequence": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNNWKLFIHIADVSYYVKENDLIDNEALLRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA" }, "paralog": false, "identity": 0.9979838709677419, "score": 972.0, "evalue": 0.0, "description": "Internal stop codon at 260072. Nonsense mutation around 260072.", "psc": { "uniref90_id": "UniRef90_D1J7X7", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_B3PMK9", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X7", "EC:3.1.13.1", "UniRef:UniRef50_B3PMK9", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK9" ], "product": "Ribonuclease R" } }, "id": "DFOKDJOJIG_495", "locus": "GCA013367855_00470" }, { "type": "tmRNA", "sequence": "contig_1", "start": 261839, "stop": 262209, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 261922, "stop": 261999, "aa": "AEEKQNKQSFVLNQMMSSNPVFAY", "nt": "GCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAA" }, "nt": "GGGGGCGTACAGGCTTCGACATACATGCAAGTTATTAAGTTGCAGTAGTTTGGCAGACTATAATGCTACTAGGCTTTTTAAGCGCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAAATTCTGGTAGGCTAATGAATAGCTCGGTTCATTGTTAGCCTTTGGAGAGCTTAGCAATTAAAGTGGCGTTTTAATTGCAAAATTATGCCAATGGTTATTATGAAGATTGTTAATTTGATAATAATATAACCAAACATAAAATTAACTAAACTGTAGATACTTAATAATGAACATGTGTGGACCGGGGTTCGATTCCCCGCGCTTCCACCA", "id": "DFOKDJOJIG_34", "locus": "GCA013367855_00471" }, { "type": "cds", "sequence": "contig_1", "start": 262419, "stop": 262601, "strand": "+", "frame": 3, "gene": null, "product": "DUF2188 domain-containing protein", "db_xrefs": [ "RefSeq:WP_020002640.1", "SO:0001217", "UniParc:UPI0002F57F90", "UniRef:UniRef100_A0A2K9YST0", "UniRef:UniRef50_B3PMJ9", "UniRef:UniRef90_A0A2K9YST0" ], "nt": "ATGGCGGTCAGATTAGTAGTAAAGCATGAAGATGGTTGGGCAGTCAAAAATCCAAAAGGAAAAAGAGCATTGAGAGTATTTAAGACTCAAAAAGAAGCTGTTCAATATGCAAAAACTTTGCAAGATACTTCATCTGTAAATGTTCAGTCTAAAGCTGGGAAGTTTAGAAAGTTCACAAGCTAA", "aa": "MAVRLVVKHEDGWAVKNPKGKRALRVFKTQKEAVQYAKTLQDTSSVNVQSKAGKFRKFTS", "aa_hexdigest": "9af3d45b90630dd907ce1f2a199efb17", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0002F57F90", "ncbi_nrp_id": "WP_020002640.1", "uniref100_id": "UniRef100_A0A2K9YST0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F57F90", "RefSeq:WP_020002640.1", "UniRef:UniRef100_A0A2K9YST0" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YST0", "uniref90_id": "UniRef90_A0A2K9YST0" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YST0", "product": "DUF2188 domain-containing protein", "uniref50_id": "UniRef50_B3PMJ9" }, "pscc": { "uniref50_id": "UniRef50_B3PMJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMJ9" ], "product": "DUF2188 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_496", "locus": "GCA013367855_00472" }, { "type": "cds", "sequence": "contig_1", "start": 262558, "stop": 263481, "strand": "+", "frame": 1, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "COG:COG0190", "COG:H", "SO:0001217", "UniRef:UniRef50_D1J7X9", "UniRef:UniRef90_D1J7X9" ], "nt": "ATGTTCAGTCTAAAGCTGGGAAGTTTAGAAAGTTCACAAGCTAAAATGGCAATATTATTAAATGGTAAAGCAATCTCTTTAGAAGTTAAAGAAGATTTAAAAAAAGCTATTGAAAAAATGCCTCAAGAAATTAGACCTACTTTAGGAATTTTACAAGTAGGCGATTTAAGTGAGTCAAATATTTATGTAAAAAATAAACTTAAATATGCTTCTGACATAGGATTAAATACAAAACTAATAAAATTGCAACAAAATGCAAGTAAATTGCAAATTTCTGAAGCAATAGAATTATTAAATAAACAAAGCACCGGATTTATAGTTCAAATTCCAATGCAAACTAATCAAATTATAGATAGTCAAGAAATATTGGATTTAATAAGCATAGATCACGATATTGATGGTCTAACCTCTTTTAATCTTGATTTAAAAAATCCTAATAATTATTTTTTATCAGCTACTGCTAGTGGAATTTTTTTACTTTTAAACAAATACAATATTCTTTATAAAAATGCAAAAATTGGAGTTATTGGTCAAAGCAAAATTGTCGGGTACCCATTGGCAAATTTCTTAGTTTTAAAAGGATATAACGTATTTAGATACAATAAAGATACTCCTAAAAATAATATTAAAGACTTGGATATAATAATTGTTGCAACCGGCACAAAAGATCCAATCACATACGACCAAGTAAAACCTGGGGCGGTTATTATCGACGTTGGAATACACAGAGATAGCAATAATAAAATTTCAGGGGATTTAAATTTTAAAGAATTTGCTAACGTTGCCTCATTTATAACCCCTGTTCCCGGAGGGGTTGGTCCAATGACTATTTCGGCATTGCTCATTAATCTCTTAAAGGCAAGTTCTTTGCAAAATCCAATTATTGTACCATATCTTAAATTTTTAAAATCATATTTTAATTAA", "aa": "MFSLKLGSLESSQAKMAILLNGKAISLEVKEDLKKAIEKMPQEIRPTLGILQVGDLSESNIYVKNKLKYASDIGLNTKLIKLQQNASKLQISEAIELLNKQSTGFIVQIPMQTNQIIDSQEILDLISIDHDIDGLTSFNLDLKNPNNYFLSATASGIFLLLNKYNILYKNAKIGVIGQSKIVGYPLANFLVLKGYNVFRYNKDTPKNNIKDLDIIIVATGTKDPITYDQVKPGAVIIDVGIHRDSNNKISGDLNFKEFANVASFITPVPGGVGPMTISALLINLLKASSLQNPIIVPYLKFLKSYFN", "aa_hexdigest": "5e7af7c499263d19742e3f7885bc37b9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7X9", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.987, "score": 579.0, "evalue": 1.73e-208, "valid": true, "gene": "FolD", "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_D1J7X9", "cog_id": "COG0190", "cog_category": "H" }, "pscc": { "uniref50_id": "UniRef50_D1J7X9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7X9" ], "product": "Bifunctional protein FolD" }, "genes": [ "folD" ], "id": "DFOKDJOJIG_497", "locus": "GCA013367855_00473" }, { "type": "cds", "sequence": "contig_1", "start": 263580, "stop": 263717, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATAGGTGTTAGTGGCATGATTGCTGCCGGTAAAAGTTCATTAGCTGAAAAACTTCATCGTTATTTTGAAGGTTCGCTTATGCTTCATGAATTTGAAGAAGACGACGAAGTATTTAATACATTTTTGAAATGA", "aa": "MIIGVSGMIAAGKSSLAEKLHRYFEGSLMLHEFEEDDEVFNTFLK", "aa_hexdigest": "0bfc1de864416f9cb21613380c6e5897", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5091.789099999999, "isoelectric_point": 4.8198556900024405 }, "id": "DFOKDJOJIG_498", "locus": "GCA013367855_00474" }, { "type": "cds", "sequence": "contig_1", "start": 263841, "stop": 264059, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01712.25" ], "nt": "ATGATTTTTTTGGATAGATTTTCAATAGAACATTATATTTTTGCAAAATTGATTTTATCTAAAAAAGGTAAAAGATATTTAGATGCATATGATGCTTTGTTTGAAAAATTAATTAGTAAAGATGAATTGCCTGATTTTGCAATATTTTTAGATATTAATTTTGAAACATTTAAAAAGAGAATATTTACACGCGGAAGGCAAAGTGAAGTAGATAACTGA", "aa": "MIFLDRFSIEHYIFAKLILSKKGKRYLDAYDALFEKLISKDELPDFAIFLDINFETFKKRIFTRGRQSEVDN", "aa_hexdigest": "579e8b1a95c98a26c99315edf116751f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01712.25", "name": "dNK", "length": 62, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 1.1997199535458725e-07, "score": 27.327556610107422, "start": 10, "stop": 69 } ], "seq_stats": { "molecular_weight": 8669.956499999993, "isoelectric_point": 7.985392570495606 }, "id": "DFOKDJOJIG_499", "locus": "GCA013367855_00475" }, { "type": "cds", "sequence": "contig_1", "start": 264380, "stop": 264718, "strand": "+", "frame": 2, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0634", "COG:F", "EC:2.4.2.8", "GO:0006166", "KEGG:K00760", "SO:0001217", "UniRef:UniRef50_L0RW64", "UniRef:UniRef90_D1J7Y1" ], "nt": "TTGGCGCAGTTAATTAAAGACGTAACTGTAGATTTTGTTTTAGATTGCATGATAACTAGCTCATATGACGGAAAAGATAGATCTTCGGGAAATGTCAAAATAACATTGGATTTACAAAATAATATTGCAGATAAAGATGTATTAATTGTTGAAGATATAGTAGATAGTGCTAGAACTATGACCAAAGTTGTTTCACTGCTAAAATCAAGAAATCCTAAATCATTAAAAGTTCTTACTTTACTTGATAAACCATCTGGCAGATTAGTAGAATTTAAACAAGATAACTTTGGTTTTGAAATCGGCAAAGATGACTTTGTTGTTGGCTTTGGCTTTGATTGA", "aa": "MAQLIKDVTVDFVLDCMITSSYDGKDRSSGNVKITLDLQNNIADKDVLIVEDIVDSARTMTKVVSLLKSRNPKSLKVLTLLDKPSGRLVEFKQDNFGFEIGKDDFVVGFGFD", "aa_hexdigest": "7ffa06ac28e5651618ac95176dd3d0e6", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "genes": [ "hpt" ], "truncated": "both", "pseudogene": { "start": 264227, "stop": 264793, "observations": { "mutation": [ 264308, 264716 ], "stop": [ 264308, 264716 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Y1", "query_cov": 1.0, "subject_cov": 0.5957446808510638, "identity": 0.982, "score": 213.0, "evalue": 1.05e-68, "gene_start": 1, "gene_end": 112, "reference_start": 52, "reference_end": 163, "reference_sequence": "MSQIDYRIKKVLFDQATLEKRIKELANWVNETYKDSKNLIIVGLLKGCLPFLAQLIKDVTVDFVLDCMITSSYDGKDRSSGNVKITLDLQNNIADKDVLIVEDIVDSARTMTKVVSLLKSRNPKSLKVLTLLDKPSGRLVEFKQDNFGFEIGKNDFVVGFGFDWEEKMRQLPYIGLLTDKEINKHKKH" }, "paralog": false, "identity": 0.9787234042553191, "score": 358.0, "evalue": 1.07e-128, "description": "Internal stop codon at 264308, 264716. Nonsense mutation around 264308, 264716.", "psc": { "uniref90_id": "UniRef90_D1J7Y1", "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_L0RW64", "cog_id": "COG0634", "cog_category": "F", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0006166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Y1", "EC:2.4.2.8", "UniRef:UniRef50_L0RW64", "COG:COG0634", "COG:F", "KEGG:K00760", "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW64" ], "product": "Hypoxanthine phosphoribosyltransferase" } }, "id": "DFOKDJOJIG_500", "locus": "GCA013367855_00476" }, { "type": "cds", "sequence": "contig_1", "start": 264804, "stop": 265160, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y2", "UniRef:UniRef90_D1J7Y2" ], "nt": "ATGATTCTTAAGTTACAAAATTGGCAAATAACAGAAACTTCAAATCAAGATAGTGGCACTAGCATACCTTTAGCTATTGGCTCTGCGTTTGTGGCATTTATTTTAATAATTGCTGTAGTTATTTTTATAATAAAAAAAATAATAAATAAGCAAACAAAAACAAAAGATAATTCAACTAATAAAGAATTAAATAAAGACAATAAAAATGACGAATATAATAAAAAAATTACATTGCCTTTTTTTAATTCTGAAGACCTAAAAAGAATAGATAAATTAAATTCTTTAAAAATTGAAAAACAAATCGCTTATTTATTTGACTCGCAAAAATTTTTTAAAGAAAATTTAAATAATTTATGA", "aa": "MILKLQNWQITETSNQDSGTSIPLAIGSAFVAFILIIAVVIFIIKKIINKQTKTKDNSTNKELNKDNKNDEYNKKITLPFFNSEDLKRIDKLNSLKIEKQIAYLFDSQKFFKENLNNL", "aa_hexdigest": "9ae4e80b8dec7d89c32eb04e74b27883", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 264750, "stop": 265463, "observations": { "mutation": [ 264756, 265158, 265278 ], "stop": [ 264756, 265158, 265278 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Y2", "query_cov": 1.0, "subject_cov": 0.4229390681003584, "identity": 0.941, "score": 208.0, "evalue": 1.57e-65, "gene_start": 1, "gene_end": 118, "reference_start": 20, "reference_end": 137, "reference_sequence": "MVYWQIKKLINTKNTNNFSIILKLQNWQITETSNQDSSTSITLAIGSAFVAFILIAAVVIFIIKKIINKQSKTRDNSTNKELNKDNKNDEYNKKITLPFFNSEDLKRIDKLNSLKIEKQIAYLFDPQKFFKENLNNLWVKKYYEIIKKFKLEKSPTSKFNKLKINKYYKNYLNEVGQWNYCRHHIDEIKISGAIYSAHPQTKEYYKTGDVILVTYEQHLFLHYLIVMAKTTMPNHGMIVGLKFHFNDTKKCIEYWDKVIQKMCEKYLVKYDKNWSENLT" }, "paralog": false, "identity": 0.9578059071729957, "score": 452.0, "evalue": 5.17e-164, "description": "Internal stop codon at 264756, 265158, 265278. Nonsense mutation around 264756, 265158, 265278.", "psc": { "uniref90_id": "UniRef90_D1J7Y2", "uniref50_id": "UniRef50_D1J7Y2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Y2", "UniRef:UniRef50_D1J7Y2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Y2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13654.654199999975, "isoelectric_point": 9.426132011413575 }, "id": "DFOKDJOJIG_501", "locus": "GCA013367855_00477" }, { "type": "cds", "sequence": "contig_1", "start": 266304, "stop": 267101, "strand": "+", "frame": 3, "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "RefSeq:WP_012855474.1", "SO:0001217", "UniParc:UPI0001BC2196", "UniRef:UniRef100_D1J7Y4", "UniRef:UniRef50_Q98QH5", "UniRef:UniRef90_D1J7Y4" ], "nt": "ATGATTGAAATTAAAAAAATATCTTATAAATATCCAGGCTCATCGAAGCTGGCGTTAGAAAATATTGATCTTAAAATTGAAGAAGGTCAATATATTGCTATTTTAGGTCATAACGGTTCGGGAAAAAGCACATTTTCTAAACTTTTATCTGCAATTTATAAACCAACATCAGGAAAAATAATAATTGATGGATTAGAAATTAATTCAGAAAATTTACTAGAAATTAGAAAAAAAATTGGAATCGTATTTCAAAATCCTGATAACCAATTTATTGGTTCAACAGTTGAAGATGATATTGCTTTTGGTCTTGAAAATAAAATGTTGAAACCTGAAGAAATGAAAACAATTATTTATAAATATGCTCAAGAGGTCGGAATGCTAGATCAGTTAGATAGAGAACCACAAAATCTATCTGGTGGTCAAAAACAACGCGTTGCAATTGCTTCAACATTAGCCCTAGATCCTAAAATAATTATTTTCGATGAAATTACTTCAATGCTAGATCCTAAAGGGCGTAATGATATTTATAAAATAATTCATAATTTACACAAAAAAACTAATAAAACATTAATTTCTATCACACATGATATGGATGAAGCACTACTAGCAGATAAATTAATAGTATTTGCTGGCGGTCATGTTATTGCTCAAGGTAAACCAATAGAAATCCTAAATAACAAAGAAATTATTGAAATTGCAAAAATAGATTCTCCATTTATCTATAAACTAAGTAGTCTCTTAAATGATATTGAACCAACATATAATAGAGAGGAATTAATTAAAGAAATATGCAAATAA", "aa": "MIEIKKISYKYPGSSKLALENIDLKIEEGQYIAILGHNGSGKSTFSKLLSAIYKPTSGKIIIDGLEINSENLLEIRKKIGIVFQNPDNQFIGSTVEDDIAFGLENKMLKPEEMKTIIYKYAQEVGMLDQLDREPQNLSGGQKQRVAIASTLALDPKIIIFDEITSMLDPKGRNDIYKIIHNLHKKTNKTLISITHDMDEALLADKLIVFAGGHVIAQGKPIEILNNKEIIEIAKIDSPFIYKLSSLLNDIEPTYNREELIKEICK", "aa_hexdigest": "5a3ed23426316973a47bd29793f9d158", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC2196", "ncbi_nrp_id": "WP_012855474.1", "uniref100_id": "UniRef100_D1J7Y4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2196", "RefSeq:WP_012855474.1", "UniRef:UniRef100_D1J7Y4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7Y4", "uniref90_id": "UniRef90_D1J7Y4" }, "psc": { "uniref90_id": "UniRef90_D1J7Y4", "gene": "EcfA2", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q98QH5", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QH5" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [ "ecfA2" ], "id": "DFOKDJOJIG_502", "locus": "GCA013367855_00478" }, { "type": "cds", "sequence": "contig_1", "start": 267092, "stop": 267391, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZL6", "UniRef:UniRef90_UPI001E584429" ], "nt": "ATGCAAATAAAAGCTTCTGAAATTACCAAAGAATACGACAAAGGATTGCCCACTTATATAAAAGTTATTGATAATGTAAGTTTAGAAATATCAGAAGGAGAAGCAATATCTATTATTGGCCCAACTGGTTCAGGGAAAACAACTCTTATTGAACATTTTAATGGTTTAATTATTCCTACAACTGGAAAAATTATCTTTGATGAGTTACCAATAAAAATTAAAACTCCAAAACCTAAAAAACCTAACTTAAAAAAAATTAAACATGATTCAAATGAATATGAAATTGTTCTTGAAAAATGA", "aa": "MQIKASEITKEYDKGLPTYIKVIDNVSLEISEGEAISIIGPTGSGKTTLIEHFNGLIIPTTGKIIFDELPIKIKTPKPKKPNLKKIKHDSNEYEIVLEK", "aa_hexdigest": "d24c92422e17ca4a85fa340f79e9ff62", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI001E584429", "query_cov": 0.8484848484848485, "subject_cov": 0.9545454545454546, "identity": 0.976, "score": 162.0, "evalue": 2.32e-50, "valid": true, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A448ZZL6" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZL6" ], "product": "Cobalt ABC transporter ATP-binding protein" }, "genes": [], "id": "DFOKDJOJIG_503", "locus": "GCA013367855_00479" }, { "type": "cds", "sequence": "contig_1", "start": 267515, "stop": 267889, "strand": "+", "frame": 2, "gene": null, "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "SO:0001217", "UniRef:UniRef50_Q4A5A4", "UniRef:UniRef90_UPI0019672E9D" ], "nt": "GTGGGAGTTGTATTTCAATTTGCTGAATATCAACTTTTTGAATCAACCATTGAAAAAGATATAATTTTTGGACCTATTTCAATGGGTGTAAGTAAAGAAGAGGCTAAAAAATTAGCACTTAAATATATTGAAATGGTTGGATTGCCTATTGATTATTTGCAAAGAAGTCCATTTAATTTATCAGGCGGTCAAAAACGTAGAGTTGCATTAGCTGGTATATTAGCAATGGAACCAAAATTTCTAGTTTTAGATGAACCAACTGCCGGGCTTGATCCACAAGGTGTCGAAGAAATGCTAAACTTATTTCACAATCTCTATTTAAATGGAAAAACGATTATTATTGTAACACATGACCTTGATAATGCTCTAAAATGA", "aa": "MGVVFQFAEYQLFESTIEKDIIFGPISMGVSKEEAKKLALKYIEMVGLPIDYLQRSPFNLSGGQKRRVALAGILAMEPKFLVLDEPTAGLDPQGVEEMLNLFHNLYLNGKTIIIVTHDLDNALK", "aa_hexdigest": "8aa26180e85591c9ad4a0a1cf01add9e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 267215, "stop": 268090, "observations": { "mutation": [ 267389, 267410, 267443, 267887 ], "stop": [ 267389, 267410, 267443, 267887 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019672E9D", "query_cov": 1.0080645161290323, "subject_cov": 0.40584415584415584, "identity": 0.8320000000000001, "score": 204.0, "evalue": 2.18e-63, "gene_start": 1, "gene_end": 124, "reference_start": 109, "reference_end": 233, "reference_sequence": "MQIKVEKISKEFDKKLPTHIQVLKDISVDIKEGEIISIIGHTGSGKTTFIEHLNALIIPDSGKITFSNVDVNKKQWYQSKNKLQLVKMDIVVEKTKRKIKNIKSLRKEVGVVFQFAEYQLFESTIEKDIIFGPVSMGVPVEEAKKLAKKYIKMVGLPESYLQRSPFNLSGGQKRRVALAGILAMQPKYLILDEPTAGLDPAGVEQMLQLFANLNKEEGKTVIIVTHDLDNALRWTKRCILFKNGEIIRDGETYEVLNDEQCLIENKLRATQLLTFVNKLKARGVKIGKVTDINQLAEQINNWKQNKGV" }, "paralog": false, "identity": 0.6095890410958904, "score": 327.0, "evalue": 4.37e-114, "description": "Internal stop codon at 267389, 267410, 267443, 267887. Nonsense mutation around 267389, 267410, 267443, 267887.", "psc": { "uniref90_id": "UniRef90_UPI0019672E9D", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q4A5A4", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019672E9D", "UniRef:UniRef50_Q4A5A4", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5A4" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" } }, "id": "DFOKDJOJIG_504", "locus": "GCA013367855_00480" }, { "type": "cds", "sequence": "contig_1", "start": 267953, "stop": 268129, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGACGACAATTTCTTAATAGAAAATAATTTAATTCCTACTAGATTATTATCATTTGTTAATGAATTAAAAAAGAATAATGTAGATGTAGGTAAGGTAACAAGTGTTGAGGATTTAGCTGAAAAAATTAATTATTACTTAACCATGAAGCGCAAAGGAGGAACAAATGAATAA", "aa": "MSDDNFLIENNLIPTRLLSFVNELKKNNVDVGKVTSVEDLAEKINYYLTMKRKGGTNE", "aa_hexdigest": "223bb9badab2c74b88c13a5afb447dfe", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6633.472400000002, "isoelectric_point": 5.315036964416504 }, "id": "DFOKDJOJIG_505", "locus": "GCA013367855_00481" }, { "type": "cds", "sequence": "contig_1", "start": 268122, "stop": 268289, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAACAATTATTGGTAAATATTTAAATTACGATACGTTTATTCACAAAATCGATCCTCGTTTCAAATTTTTAACAAATGTTTTATTAATAATATTGCTTTTTATGGCTGATCACTTTATAACTTTGGGAATTTTAATTTTCTCAATAATGATTTTTTATTGA", "aa": "MNKTIIGKYLNYDTFIHKIDPRFKFLTNVLLIILLFMADHFITLGILIFSIMIFY", "aa_hexdigest": "4d86250178570e8cd34577cd44f37af2", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6584.976999999995, "isoelectric_point": 9.224603843688964 }, "id": "DFOKDJOJIG_506", "locus": "GCA013367855_00482" }, { "type": "cds", "sequence": "contig_1", "start": 268326, "stop": 268496, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCTTGCCACTATATATTGCAATATTTCTATTGATAATAAATATGTTTACAGTTAAAGGCGCAGTCTCTTTAAATTCGGTTTTAAGTTATGTTCCGCTTGCTTCTGATGGAAATTTAGACTCCTATGTCTATTATAGAAATGAAACAATAATTAATACAATTTATTGA", "aa": "MRLPLYIAIFLLIINMFTVKGAVSLNSVLSYVPLASDGNLDSYVYYRNETIINTIY", "aa_hexdigest": "934051d67e86686d6d8a16d23088c9ab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6377.423500000001, "isoelectric_point": 5.875412559509278 }, "id": "DFOKDJOJIG_507", "locus": "GCA013367855_00483" }, { "type": "cds", "sequence": "contig_1", "start": 268698, "stop": 268955, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTTCAATATCATTACGTTTTATCCCAACATTACTTGAAGAATCTGGTCGTATCATGAAAGCACAAGCAAGTAGAGGTATCGATTTTAAAAATGGAAATTTAAAGGATAAGACTAAATCCTTTATTGTTTTGATTGTTCCACTATTTGTTTCTTCATTTTCAAAGGCGAATGATTTGGCTGACGCAATGACATCAAGAGGTTATGAACCTTATGCTAAAAGATCTCACTATAGAGTGTTAACGCCTTGTTGA", "aa": "MIISISLRFIPTLLEESGRIMKAQASRGIDFKNGNLKDKTKSFIVLIVPLFVSSFSKANDLADAMTSRGYEPYAKRSHYRVLTPC", "aa_hexdigest": "da2f200843a255fb9cb4ff44b923710f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9555.090599999994, "isoelectric_point": 9.83956775665283 }, "id": "DFOKDJOJIG_508", "locus": "GCA013367855_00484" }, { "type": "cds", "sequence": "contig_1", "start": 269293, "stop": 270765, "strand": "+", "frame": 1, "gene": null, "product": "DNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5IJW6" ], "nt": "TTGGCTTCCGATCTATTTAAAAAAGTAAAACATAAACGTCCGATGCTTTCATTAAATAAAGCATATTCGTTAGATGAAATTCAAAAATTTATTGATAATATAAAGAAAATTACGTCTGATTTTTCTTTTTTTATTGAGCCTAAAATTGATGGCCTTTCAATTTCTATTATTTACGAAGATGGCAAATTAGTTAGAGCAATAACAAGAGGAGATGGAATAACTGGCGAAGATGTTACGGCAAATGTTGTTCAAATGTCTTGCATCCCTAAAACTATTGAATATAAAGAATCATTAGAGGTAAGGGGGGAAGTTTATTTAGCAATATCTGATTTTGAAAAATTAAATTTAAAATTAGGGCAAGAAAATAAAAATAAACTTGCAAATCCTAGAAATGCTGCAGCAGGCACATTAAGACAATTAAATTCTCAAATTATTAAAGAACGAAATTTGTCAGCATTTTTGTATTATGTTGTAGATCCTTTGCAACATAATATTAAAACAATGCAAGAAGAACATGAATTCTTAAAAACTTATGGGTTTCCCGTTACTCCCGAAGTTCAACATGCTAATACATTGCAAGATATTGAAGAATATATTAGAAACTTCAAGAATCAGAAATTATTAATAGATTATGAAACAGATGGAGTTGTTATAAAGTTAAACGAGGTTCAATATTTTGACAAGTTAGGATCAACAGCTAAGTTCCCCCATTATGCAATTGCATTTAAATATGAACCTAATGTTGAAACAACAACAATAAAAAGAATATATGCGACAGTAGGAAGAACTGGATTGGTTACATACAATGCAGAATTAGAACCAGTAGAAATTTCGGGTAGTCTTGTAAGCTTTGCAACGTTGAATAATTTCCAATACATAAAAGATTTGAATTTAAATGAAAATGATTTAGTATATATTAAAAAAGCTGGAGAGATTATTCCTTGTGTAATTGGCTTAATTTCTGGCAAAAAAGAAATTGATCATTTTCAAAAAGTAGAATTCTGCCCATATTGTCACAATAAACTTGTCGATTCAGAAACGGGATTGGAGCAATTTTGTTCAAATGATCAATGCCCCGAAATACAAAGAAGAAAATTGATTCATTTTTGTTCAAAGGATGGTTTAGATATTAATACTATGGGTGAGAGAAATATTGATCTATTTTTAAAAAATTCTCTAATTTCTAACCCTATTGATATTTTTAATTTAAAGAACAAAAAACAAGATTTATTAAAATTAAATAGAATGGGTGAAAAGTCTATTGAGAATATCTTAATTTCTATTGATGAATGTAAGAATAAACCTTTATATAAAATGATTTCTGCTCTTTCAATCCCACTAATTGGCGTAAAATTAGCTAAATTCGTAGCCTCAAAAATTAAATCATTACATAATTTATTAAATTATGATTTTTCACAATTAATTGATTATCATGAAATAGGAGATAAAATTATAAATTCTATAATTTCTTGA", "aa": "MASDLFKKVKHKRPMLSLNKAYSLDEIQKFIDNIKKITSDFSFFIEPKIDGLSISIIYEDGKLVRAITRGDGITGEDVTANVVQMSCIPKTIEYKESLEVRGEVYLAISDFEKLNLKLGQENKNKLANPRNAAAGTLRQLNSQIIKERNLSAFLYYVVDPLQHNIKTMQEEHEFLKTYGFPVTPEVQHANTLQDIEEYIRNFKNQKLLIDYETDGVVIKLNEVQYFDKLGSTAKFPHYAIAFKYEPNVETTTIKRIYATVGRTGLVTYNAELEPVEISGSLVSFATLNNFQYIKDLNLNENDLVYIKKAGEIIPCVIGLISGKKEIDHFQKVEFCPYCHNKLVDSETGLEQFCSNDQCPEIQRRKLIHFCSKDGLDINTMGERNIDLFLKNSLISNPIDIFNLKNKKQDLLKLNRMGEKSIENILISIDECKNKPLYKMISALSIPLIGVKLAKFVASKIKSLHNLLNYDFSQLIDYHEIGDKIINSIIS", "aa_hexdigest": "f09cfee4959ac0f9af0e0fd9ba69458b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BDB6CC", "query_cov": 0.9938775510204082, "subject_cov": 0.8513986013986014, "identity": 0.604, "score": 605.0, "evalue": 1.39e-211, "valid": false, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_A0AAJ5IJW6", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAJ5IJW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5IJW6" ], "product": "DNA ligase" }, "genes": [], "id": "DFOKDJOJIG_509", "locus": "GCA013367855_00485" }, { "type": "cds", "sequence": "contig_1", "start": 270856, "stop": 271077, "strand": "+", "frame": 1, "gene": null, "product": "BRCT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE292E9" ], "nt": "TTGGAAAATAAATCATTTGTAATAACAGGAACATTGGGACACCCTAGGTCATATTTTGAAAGCTTAATTGAAAAAAATGGTGGTTTAATTCACTCAAGTATTTCATCTAAAACTAATTATTTATTAGCAGGTGAAAACGCCGGATCTAAATTGCAAAAGGCAAATAAATTAAATATTACTATAATAACTGAAGAAGATTTATTTAAAATGATTGGTCAATAA", "aa": "MENKSFVITGTLGHPRSYFESLIEKNGGLIHSSISSKTNYLLAGENAGSKLQKANKLNITIITEEDLFKMIGQ", "aa_hexdigest": "518aaf48f0c2d27abdd34c553d511c7b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE292E9", "query_cov": 0.9863013698630136, "subject_cov": 1.0, "identity": 0.667, "score": 95.5, "evalue": 1.75e-24, "valid": false, "product": "BRCT domain-containing protein", "uniref50_id": "UniRef50_UPI002FE292E9" }, "pscc": { "uniref50_id": "UniRef50_UPI002FE292E9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE292E9" ], "product": "BRCT domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_510", "locus": "GCA013367855_00486" }, { "type": "cds", "sequence": "contig_1", "start": 271054, "stop": 271701, "strand": "-", "frame": 1, "gene": null, "product": "MAG0480 family ComEC-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y8", "UniRef:UniRef90_D1J7Y8" ], "nt": "TTGAATTCAAAGGAATTGATTGTTGATTTGATTTCAATATTAATGTTATTTTTTTACCTAAAATTATTAAATTTTTCAATATCTGCAAGTCGGGCGTTTTTGTTTTTGATATTAAAAATTGCAAGCAAAAACATTTTTAAAGGCAAATTTAATAACATTGACATATTGAGTTTTGCAGCACTATTATTGATATTCTTTGATATTAGAACCATGTATTCTTATTCTTTTATTTTATCTTTTACAATAACATTAATTATTTTTTTGATTTTAGACATTAAAAAAATTTACAAAAACAATATATTTGTAAATTTTCTATTTATTATATTACCTTATATTTCTTCTATTTCAATAGTAATTTCATTTAATCCAAAATTTAACATTTTAGGAATATTTTATCAATTATTCTTTCTACCAATTTCAATATTTAACTATGTATTTTGTTTATTTGCTTTTATAGTTAAACCTATAATAAAAATGTATGTTTCAATATTTGAAATAGTAATAAATACTTTAAGCTATGGTTGTTTGATTATTGAAGTAGAAAAAAATTCAATATTAGGATATTCTATTAATTTTTATATTATTTCAATATATACATATATTAAAATAAAGAGAGAATATTATTTATTGACCAATCATTTTAAATAA", "aa": "MNSKELIVDLISILMLFFYLKLLNFSISASRAFLFLILKIASKNIFKGKFNNIDILSFAALLLIFFDIRTMYSYSFILSFTITLIIFLILDIKKIYKNNIFVNFLFIILPYISSISIVISFNPKFNILGIFYQLFFLPISIFNYVFCLFAFIVKPIIKMYVSIFEIVINTLSYGCLIIEVEKNSILGYSINFYIISIYTYIKIKREYYLLTNHFK", "aa_hexdigest": "f4773617e8c4e411f505ff522b40e661", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 271054, "stop": 272001, "observations": { "mutation": [ 271704 ], "stop": [ 271704 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Y8", "query_cov": 1.0, "subject_cov": 0.4725274725274725, "identity": 0.986, "score": 379.0, "evalue": 6.95e-129, "gene_start": 1, "gene_end": 215, "reference_start": 241, "reference_end": 455, "reference_sequence": "MTGIGKFTRSRWKNFNYIKKSFNNLNTNLFVALSFCLYCLIIYKINIVISSIMLSLIIAVNFFNLKYWIKIAIVFPLIFLVHYLWYLDLFKINGKNICGKFEIKRFFNKSILIKYQYYNVLVKNWSSWEFNNFYKYNIELSGTLSIIKNQNMFSLSNKIDFELLNVKLVSKAYAYQEKIPILQDYDYKTKKFLALVLFNIQQANDLFVDQLKQLSVMHYFTISGFHFGIIYLLISKILKWLNSKELVVDLISILMLFFYLKLLNFSISASRAFLFLILKIASKNIFKGKFNNIDILSFAALLLIFFDIRTMYSYSFILSFTITLIIFLILDIKKIYKNNIFVNFLFIILPYISSISIVISFNPKFNILGIFYQLFFLPISIFNYVFCLFAFIVKPIIKMYVSIFEIIINTLSYGCLIIEVEKNSILGYSINFYIISIYTYIKIKREYYLLTNHFK" }, "paralog": false, "identity": 0.9873015873015873, "score": 603.0, "evalue": 2.93e-219, "description": "Internal stop codon at 271704. Nonsense mutation around 271704.", "psc": { "uniref90_id": "UniRef90_D1J7Y8", "product": "MAG0480 family ComEC-like protein", "uniref50_id": "UniRef50_D1J7Y8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Y8", "UniRef:UniRef50_D1J7Y8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Y8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y8" ], "product": "ComEC/Rec2-related protein domain-containing protein" } }, "id": "DFOKDJOJIG_511", "locus": "GCA013367855_00487" }, { "type": "cds", "sequence": "contig_1", "start": 271702, "stop": 272004, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGGCACTTTATCAATAATTAAAAATCAAAATATGTTTAGTTTGAGCAATAAAATTGATTTTGAATTGTTAAATATTAAATTGGTTTCAAAAGCATATGCATATCAAGAAAAAATTCCAATATTGCAAGATTATGATTATAAAACAAAAAAATTTCTAGCACTTGTTTTATTCAACATTCAACAAGCTAATGATTTGTTTGTAGATCAATTAAAACAACTAAGTGTTATGCACTATTTTACAATAAGCGGATTTCATTTTGGAATAATTTATTTGCTTATATCAAAAATTTTAAAATGA", "aa": "MSGTLSIIKNQNMFSLSNKIDFELLNIKLVSKAYAYQEKIPILQDYDYKTKKFLALVLFNIQQANDLFVDQLKQLSVMHYFTISGFHFGIIYLLISKILK", "aa_hexdigest": "82ca36423656e8b2f32def07f57c8825", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11671.681799999993, "isoelectric_point": 9.331943702697757 }, "id": "DFOKDJOJIG_512", "locus": "GCA013367855_00488" }, { "type": "cds", "sequence": "contig_1", "start": 272418, "stop": 272858, "strand": "-", "frame": 2, "gene": null, "product": "MAG0490 family ComEA-like DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y9", "UniRef:UniRef90_D1J7Y9" ], "nt": "ATGAATAAACGAAAAATTTTTTTGGGACTAGGATTATTTTCATCAGCATCAATAATAATACTAATGTCAATAGGAATTAATAAATACATTTCAAATATAAAAAATAACAATAAATTATTTATCAAAAACAAAAATGAAATAATAACAATAGGAATATCAGGCGCAATAGAGTATCCTGACTCATATGAAATTAAAAAAGGCACTAAATTATTGGAAATAATAAAACAAGCACGTCTTAAAACGGGTGCAGATTTAAACAATATTGATTTAAATTTAAAATTAGAAAAAAATCAATCAATTAATATTCCTTTTAAAAAAGATAATTCAATTTCTATTAGAGAAGTCAATAACGTTGGCATATTAGAGGATTTAGGAATAAAAAAAGATATAGCAAAAATTATTTTAAGCTACTTCATTGCTAAGGATTATTTAATAACATGA", "aa": "MNKRKIFLGLGLFSSASIIILMSIGINKYISNIKNNNKLFIKNKNEIITIGISGAIEYPDSYEIKKGTKLLEIIKQARLKTGADLNNIDLNLKLEKNQSINIPFKKDNSISIREVNNVGILEDLGIKKDIAKIILSYFIAKDYLIT", "aa_hexdigest": "05cca158ff3690d2036121b2aad0a68e", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_D1J7Y9", "query_cov": 1.0, "subject_cov": 0.8588235294117647, "identity": 0.9790000000000001, "score": 265.0, "evalue": 3.12e-89, "valid": true, "product": "MAG0490 family ComEA-like DNA-binding protein", "uniref50_id": "UniRef50_D1J7Y9" }, "pscc": { "uniref50_id": "UniRef50_D1J7Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y9" ], "product": "ComEA family DNA-binding protein" }, "genes": [], "id": "DFOKDJOJIG_513", "locus": "GCA013367855_00489" }, { "type": "cds", "sequence": "contig_1", "start": 272999, "stop": 273640, "strand": "-", "frame": 3, "gene": "tACO1", "product": "YebC/PmpR family DNA-binding transcriptional regulator", "db_xrefs": [ "COG:COG0217", "COG:KJ", "SO:0001217", "UniRef:UniRef50_Q98PU5", "UniRef:UniRef90_D1J7Z0" ], "nt": "ATGTTTCAAAAATTTTCAAAAGAAATTATTGTTGCTGCAACATTAGGCGGACCAGATCCAGACTCTAACCCATCGCTAAAATTAGCAATTGCTAAGGCTAAGGCAAAAAGTATGCCTAAAGCAAACATTGAAAAGGCAATTGCAAAAGTAGCTGGCGGATCTAAAGAAGCTGCAAACTATCAATCTTATTTATATTCAGGTACAGCATTTGGCGGTGTAAATTTTATTGTAAGTTGTTTAACAGATAATTTCAATCGTTTAAGCTCGAATATCAAACATTTCTTTTCAAAACACAATGGTCAATTAGGAAAACAAGGAATAATACCATATGTTTTTGATCAAAAAGGTTTAATTGAATTTCCAAGTAATGTTTCTGGTGAAGAAGAAACCATGATGACAGTTTTAGATGCGGGAGCAGAAGATTTTAGAAATGAAGATGGAACTTATACAATAATTACAAGCCCTTCAGCATTTCAAAAAGTAAAACAAGCATTAGATGAAACATTCAAAATTGAAAATTATACAACAGCTGAAGTTACATATATCCCAAATACCGAAATTGAAGTAGATGAAGAAAAAGCAAGCCAAATTGAACAATTTGTTGACTTTTTAGAAGACGATGAAGACGTCCAAGAAGTTTGA", "aa": "MFQKFSKEIIVAATLGGPDPDSNPSLKLAIAKAKAKSMPKANIEKAIAKVAGGSKEAANYQSYLYSGTAFGGVNFIVSCLTDNFNRLSSNIKHFFSKHNGQLGKQGIIPYVFDQKGLIEFPSNVSGEEETMMTVLDAGAEDFRNEDGTYTIITSPSAFQKVKQALDETFKIENYTTAEVTYIPNTEIEVDEEKASQIEQFVDFLEDDEDVQEV", "aa_hexdigest": "40ff67e088fb0f542b927f88be3c8775", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_D1J7Z0", "query_cov": 1.0, "subject_cov": 0.8765432098765432, "identity": 1.0, "score": 400.0, "evalue": 2.78e-140, "valid": true, "gene": "TACO1", "product": "YebC/PmpR family DNA-binding transcriptional regulator", "uniref50_id": "UniRef50_Q98PU5", "cog_id": "COG0217", "cog_category": "KJ" }, "pscc": { "uniref50_id": "UniRef50_Q98PU5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PU5" ], "product": "Probable transcriptional regulatory protein MYPU_6240" }, "genes": [ "tACO1" ], "id": "DFOKDJOJIG_514", "locus": "GCA013367855_00490" }, { "type": "cds", "sequence": "contig_1", "start": 273794, "stop": 274756, "strand": "+", "frame": 2, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "RefSeq:WP_044329493.1", "SO:0001217", "UniParc:UPI0005C9D0EE", "UniRef:UniRef100_UPI0005C9D0EE", "UniRef:UniRef50_A0A454C9E8", "UniRef:UniRef90_A0A454C9E8" ], "nt": "ATGTATTTAATAAGAGGAGAAGATAGTTATTTATTAAATCAAGAATTACAAAAAATTATTGATTTAAAATTTGAAAATCAAGACGATTATTCAGTAATCACTTACAATGAATCTATTGATTTAGAACAACTATCAGATGATATATTTAACCAATCATTATTTTCTTTTAAAAAACAAGTCATAGTTTTAAAAAACATTGATTTTTTTAATTCTAAGAAAAAAAATGACAATAAAAAAATGAACGATTTTTTACAATTGCTTGAAAAAGGTCAAGATAACAATTTAATATTTATTTTGCAGGATATTAAAAAAAATGATATTTCTTTTAATCCTTCATTGGCTTATAAAAAAATTGAATATATTTCAAAAATAATTGAAATTAAAAAACCAAGTGATAAGGATGTATATAACTATATAAAGGAATATATTTTAAAAAATGGAGGCAACATATCTTCAATTACATTACTTGATTTTTTGTCAATGTTTCCTAATGATTTACAACAAATCATTAATGAAGTAGATAAATTGCTATTGCAAAACAAAAATATTGATATGTTGTTGATCAATAATTCAAATATTATTTCTTCTGACAACATTGAATTTAATTTATCAAATTGTATTTTAAAACAAAAAAGCATTGATATTATAGAAGCAGTAGATGAACAAAAGAAATATGGTAATGGTGCTAGTGAAATTCTAAGCCAAATAAGTTATTTGTTTTATCAAATTTATTGCACTTATTTGTTGATGGAAAATACCAGTGATAATGAATATATATCAAAAACTTTATCAATACATTCATATAGAATCAAGTTGTTTATGTCGTATATAAAAAAAACAAACAAGTATAAATTAAAAGAATTAATAGTGTTACTTGCTGATATAGATATAGCAATTAAATCAGGATTATTAGATGAAGAAAATGCCTTAAATAAATTTATTTTATTTTACATAGAAATATAA", "aa": "MYLIRGEDSYLLNQELQKIIDLKFENQDDYSVITYNESIDLEQLSDDIFNQSLFSFKKQVIVLKNIDFFNSKKKNDNKKMNDFLQLLEKGQDNNLIFILQDIKKNDISFNPSLAYKKIEYISKIIEIKKPSDKDVYNYIKEYILKNGGNISSITLLDFLSMFPNDLQQIINEVDKLLLQNKNIDMLLINNSNIISSDNIEFNLSNCILKQKSIDIIEAVDEQKKYGNGASEILSQISYLFYQIYCTYLLMENTSDNEYISKTLSIHSYRIKLFMSYIKKTNKYKLKELIVLLADIDIAIKSGLLDEENALNKFILFYIEI", "aa_hexdigest": "9adb61e60052f3c230ce08f151d3f99d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0005C9D0EE", "ncbi_nrp_id": "WP_044329493.1", "uniref100_id": "UniRef100_UPI0005C9D0EE", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C9D0EE", "RefSeq:WP_044329493.1", "UniRef:UniRef100_UPI0005C9D0EE" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C9D0EE", "uniref90_id": "UniRef90_A0A454C9E8" }, "psc": { "uniref90_id": "UniRef90_A0A454C9E8", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A454C9E8", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9E8" ], "product": "DNA polymerase III subunit delta" }, "genes": [ "holA" ], "id": "DFOKDJOJIG_515", "locus": "GCA013367855_00491" }, { "type": "cds", "sequence": "contig_1", "start": 274767, "stop": 274952, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTTCTATTTATGAACTTAATTTAAGCACTTTTAATAATAAAAAGGGTTTAAACAAGTATTTGTCAATTATTGAAAAAATTGAATATTTAAAATCATTAAATATTGATATCTTAGCAATTGATGATATATTATTGAAATGCTATGAAATCAATGGAAATTATGAATTTAATGATTGA", "aa": "MKKISIYELNLSTFNNKKGLNKYLSIIEKIEYLKSLNIDILAIDDILLKCYEINGNYEFND", "aa_hexdigest": "8459dc6096d0c9fd4c44d758ac208b5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7191.274599999999, "isoelectric_point": 5.282127189636229 }, "id": "DFOKDJOJIG_516", "locus": "GCA013367855_00492" }, { "type": "cds", "sequence": "contig_1", "start": 274959, "stop": 275363, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATTTTAAAAAATTAGTTGAACTTTGTTCAGCCAATAACATTAAAATAATGCCAACAATCAATATTGCAAAATTAAAAAAAAGTATTATTGCAAAAAAGCATTTAATTTCTATTTATCGAAACAAAAGCAGTGAAAATGAATCAAATAAAAATGATATTGAATCTTATTTATTAACCCAAGAATTTCTAGTTCCCACCATTGAAAATATTTCAAATTTAAGCGTTTTCTTAAAAGAAATTATTAGTTTTTATAGTGTATTAGGTATTAAGGGATTTATTCTTGAGGATTTTGAATTTTTAATTGACAAAAAAACAAATAACATAAAGTCAAAATTTAGTTTTTTATTAGATATATATAGAATTATCAAACACATAAATTTTAGTTCTATGGTTATTTGA", "aa": "MQDFKKLVELCSANNIKIMPTINIAKLKKSIIAKKHLISIYRNKSSENESNKNDIESYLLTQEFLVPTIENISNLSVFLKEIISFYSVLGIKGFILEDFEFLIDKKTNNIKSKFSFLLDIYRIIKHINFSSMVI", "aa_hexdigest": "7955d6738e299291088ad0c1f4c2077c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15569.179099999988, "isoelectric_point": 9.277983665466309 }, "id": "DFOKDJOJIG_517", "locus": "GCA013367855_00493" }, { "type": "cds", "sequence": "contig_1", "start": 275664, "stop": 276140, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCAAAAAATTCTTATAGTTTATACAATGGCGATGAATTAGGAATTCTTGGTACTGATTCAAAGAATATTTTTAATTTTAATAACATTAATTTTAACGAAGAAAAAAGATTTTATCAATCCAAAAATATTTCTAGTCAAAAATATATTGAGTCTCAAATTAAATTCAATCCAATAACACTACAAATAGAATTTCCATGGACTAATAACATTCAAAATTTAGATTTTATACAGCCGAAATTGCGTAACAAAATCAATGTTGAACAACAGTTAACCAGTAAAGATAGCACGTTTAATTTTTATTCAAATTTAGTTAGAATTTTAAACAAATCAAAATATTCAATTTATTTTAAGGGTTTTAACAAAGTTTTACTTTGCAATAATTTAATAAGTATTGAATATAGTACTAGTGAAAATAAAAAACTTTTAATTTTATTAAACTTGAATTCATTTCCAATAAAATCATATATATGA", "aa": "MLAKNSYSLYNGDELGILGTDSKNIFNFNNINFNEEKRFYQSKNISSQKYIESQIKFNPITLQIEFPWTNNIQNLDFIQPKLRNKINVEQQLTSKDSTFNFYSNLVRILNKSKYSIYFKGFNKVLLCNNLISIEYSTSENKKLLILLNLNSFPIKSYI", "aa_hexdigest": "fce56266c34cb44f82a0cb8e11c96fa1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18595.028000000013, "isoelectric_point": 9.426518821716307 }, "id": "DFOKDJOJIG_518", "locus": "GCA013367855_00494" }, { "type": "cds", "sequence": "contig_1", "start": 276300, "stop": 276422, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTACAATTCGATGCATCACAAGTAGCAAAGGAACAAAAGGCGTCAACAGTTGGTTCTGCAATTGTTTATACACTTTTAGCTATAGTTTTGGCAGCCGTCTTAGCTTTTTTAATTTGA", "aa": "MQLQFDASQVAKEQKASTVGSAIVYTLLAIVLAAVLAFLI", "aa_hexdigest": "91c0d7081103885315333806e60440c0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4222.982499999998, "isoelectric_point": 5.8228364944458 }, "id": "DFOKDJOJIG_519", "locus": "GCA013367855_00495" }, { "type": "cds", "sequence": "contig_1", "start": 276465, "stop": 276737, "strand": "+", "frame": 3, "gene": null, "product": "DUF4129 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Z3", "UniRef:UniRef90_D1J7Z3" ], "nt": "GTGGAAATGCAAAAACAAAAAGAAACGTTAGCACTATTTTATCAATATATTTTAAGCTTTTATGAAGTTATAAAATTTACAAACCAAGAACTTAAAAATTTTGAAGTTTCAATAAGCACTCATCCAATGGGTGAAATTAAAGAAGGCGCTAAAAGGTTACTATATCGTTTAATTACAAGAGATGATTTTTCATATTCATTTGTTAAAAATGAACAATACAAAACCTTTGTAAAACACGCCGAATTAATAAATATTACAAATTGCAACTTATGA", "aa": "MEMQKQKETLALFYQYILSFYEVIKFTNQELKNFEVSISTHPMGEIKEGAKRLLYRLITRDDFSYSFVKNEQYKTFVKHAELINITNCNL", "aa_hexdigest": "3b270cbbb7ca55059e129c7a92f495b4", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 276300, "stop": 276869, "observations": { "mutation": [ 276420, 276735 ], "stop": [ 276420, 276735 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Z3", "query_cov": 0.9888888888888889, "subject_cov": 0.4708994708994709, "identity": 1.0, "score": 179.0, "evalue": 1.07e-55, "gene_start": 2, "gene_end": 90, "reference_start": 57, "reference_end": 145, "reference_sequence": "MQLQFDASQVAKEQKASTVGSAIVYTLLAIVLAAVLAFLIWKLIKNKVLKKRAQKVEMQKQKETLALFYQYILSFYEVIKFTNQELKNFEVSISTHPMGEIKEGAKRLLYRLITRDDFSYSFVKNEQYKTFVKHAELINITNCNLWDKKIPETINYFKEQYELVPLGKTRDDYIQLVQKSISERFYNEK" }, "paralog": false, "identity": 0.9841269841269841, "score": 358.0, "evalue": 6.86e-129, "description": "Internal stop codon at 276420, 276735. Nonsense mutation around 276420, 276735.", "psc": { "uniref90_id": "UniRef90_D1J7Z3", "product": "DUF4129 domain-containing protein", "uniref50_id": "UniRef50_D1J7Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Z3", "UniRef:UniRef50_D1J7Z3" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Z3" ], "product": "DUF4129 domain-containing protein" } }, "id": "DFOKDJOJIG_520", "locus": "GCA013367855_00496" }, { "type": "cds", "sequence": "contig_1", "start": 277081, "stop": 277317, "strand": "+", "frame": 1, "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "db_xrefs": [ "EC:2.7.8.5", "GO:0008444", "GO:0008654", "GO:0016020", "SO:0001217", "UniRef:UniRef50_UPI001E3AA40F", "UniRef:UniRef90_UPI001E3AA40F" ], "nt": "ATGATAACTGATTTCTTAGATGGATTTTATGCAAGGAAAACAAAAACCGTATCCTCATTTGGAAAGATATTTGATCCAATTGCTGATAAAGTTGCAACTACATTGATGTTACTATTCTTAGCGACATATAATTTTGTTTATATACCTATTGCAATTTTGTTTATTATAAGAGACATAATTGTTGATGGTACTAGAGTATATGCTGTAAAGAAAAACATTGAAGTATCAGCAAATTGA", "aa": "MITDFLDGFYARKTKTVSSFGKIFDPIADKVATTLMLLFLATYNFVYIPIAILFIIRDIIVDGTRVYAVKKNIEVSAN", "aa_hexdigest": "4a35e5d28719adca9e49fa9caef55052", "start_type": "ATG", "rbs_motif": null, "genes": [ "pgsA" ], "truncated": "both", "pseudogene": { "start": 276859, "stop": 277393, "observations": { "insertion": [ 277375 ], "mutation": [ 276919, 277051, 277315 ], "stop": [ 276919, 277051, 277315 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E3AA40F", "query_cov": 1.0, "subject_cov": 0.4406779661016949, "identity": 0.9620000000000001, "score": 144.0, "evalue": 2.15e-42, "gene_start": 1, "gene_end": 78, "reference_start": 75, "reference_end": 152, "reference_sequence": "MKNKSQKKQKIIVSKFGVANWLTVLRMLLMIPFIVFLSTSFGLILNGVQLSYKTNKGIVLSILYWINVAIFIIAMITDFLDGFYARKTKTVSSFGKIFDPIADKVATTLMLLFLATYNFVYIPIVILFIIRDIIVDGTRVYAIKKNIEVSTNWFGKIKTILVSLALVVISIGFLLYR" }, "paralog": false, "identity": 0.96045197740113, "score": 313.0, "evalue": 2.59e-111, "description": "Internal stop codon at 276919, 277051, 277315. Frameshift due to insertion around 277375. Nonsense mutation around 276919, 277051, 277315.", "psc": { "uniref90_id": "UniRef90_UPI001E3AA40F", "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "ec_ids": [ "2.7.8.5" ], "uniref50_id": "UniRef50_UPI001E3AA40F", "go_ids": [ "GO:0008444", "GO:0008654", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E3AA40F", "EC:2.7.8.5", "UniRef:UniRef50_UPI001E3AA40F", "GO:0008444", "GO:0008654", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E3AA40F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3AA40F" ], "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase" } }, "id": "DFOKDJOJIG_521", "locus": "GCA013367855_00497" }, { "type": "cds", "sequence": "contig_1", "start": 277513, "stop": 277623, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTCAATCATATACAAAAAATGATTCAAATGCAACAAATAAAAATAATCAAGATCAAAATAATAATAATAAATCAAATCAAGATTCTAGCCCATCGCCTAATTGCTAA", "aa": "MLQSYTKNDSNATNKNNQDQNNNNKSNQDSSPSPNC", "aa_hexdigest": "96ebcd4fa8c001ff002d11967736b77d", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4016.0894000000008, "isoelectric_point": 5.791518211364747 }, "id": "DFOKDJOJIG_522", "locus": "GCA013367855_00498" }, { "type": "cds", "sequence": "contig_1", "start": 277779, "stop": 278123, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B181AC3", "UniRef:UniRef90_UPI000B181AC3" ], "nt": "ATGAAATCAAAGAAAATAAACTTATTAAAATTATTATTAACATCGTCAGTTGCAATTTCGTGTGCAATATTACCTGTTTCTTGTTCTTTATTATCTAATGATAATGATAAAAAAATAACAATAAAAAATTATGAATTTAATAATTTATCAAGTGAATACACAATTGAATCTAAAAAATTAAATTTATCATTTGTTAATGATGGCAATGTTCCATATGTTAATTTGCTACAAATGATAAATTCTTTAGATGGATTTTTTGATAGAAATTATATTAATTTTTCGACAAATATTCTATTTTCTGAATTAAAAATGACAACTAAATTTGGATATATTTCGTTTAACTGA", "aa": "MKSKKINLLKLLLTSSVAISCAILPVSCSLLSNDNDKKITIKNYEFNNLSSEYTIESKKLNLSFVNDGNVPYVNLLQMINSLDGFFDRNYINFSTNILFSELKMTTKFGYISFN", "aa_hexdigest": "15dfa7022abbecdf7f17f4e25103b6fd", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI000B181AC3", "query_cov": 1.0, "subject_cov": 0.8837209302325582, "identity": 0.991, "score": 216.0, "evalue": 9.91e-71, "valid": true, "uniref50_id": "UniRef50_UPI000B181AC3" }, "pscc": { "uniref50_id": "UniRef50_UPI000B181AC3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B181AC3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12966.863299999983, "isoelectric_point": 8.886015892028809 }, "id": "DFOKDJOJIG_523", "locus": "GCA013367855_00499" }, { "type": "cds", "sequence": "contig_1", "start": 278142, "stop": 278669, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D962DE", "UniRef:UniRef90_UPI0034D962DE" ], "nt": "ATGCCAAACACTTATTATGAATATATAACAAAAAGCCCAGGAAAGACTAATTTCAACAAATATTTGAAATCCAAATCATATTATTCAAAGGATTTTAATAAAAATACAATTGTTATAGATTTAAAAAAATATAATTTTGACATTTTATATCATAACGGTAATGTTTTGCTACCATTAGCAATATTTAATTTGTTATTTTGCTCAACTAATTATTACAATTTGTATTTCAATGGTAACAAAATCTATGGGTGCGATTTTGACATCAAATCTAATGTGCAAGAAATTCCTCAAATTAGAAATAGCAAATTTCAAAACTCACTTATTCCCAAGGATATAGTTGAAGCAAAATTTAACTTCACTGCTTTACTAATTGATTATTTTTACGGCCTTAAAGAATATAATAATATTAATAGTTTTTATTCTACAATTTCAAATGAACAAAAGCAAAGAATGCTTAGTCAAAATCCAGTTATTTCAACAAAGGAATATATAAAATATTTATACAATTTAAATGATTTACACACCTGA", "aa": "MPNTYYEYITKSPGKTNFNKYLKSKSYYSKDFNKNTIVIDLKKYNFDILYHNGNVLLPLAIFNLLFCSTNYYNLYFNGNKIYGCDFDIKSNVQEIPQIRNSKFQNSLIPKDIVEAKFNFTALLIDYFYGLKEYNNINSFYSTISNEQKQRMLSQNPVISTKEYIKYLYNLNDLHT", "aa_hexdigest": "c7d7aca0dccb228f1dfa1a775d19b126", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 277842, "stop": 278729, "observations": { "mutation": [ 278121, 278667 ], "stop": [ 278121, 278667 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D962DE", "query_cov": 1.0, "subject_cov": 0.5537974683544303, "identity": 0.983, "score": 347.0, "evalue": 1.11e-118, "gene_start": 1, "gene_end": 175, "reference_start": 122, "reference_end": 296, "reference_sequence": "MKSKKINLLKLLLTSSVAISCAILPVSCSLLSNDNDKKITIKNYEFNNLSSEYTIESKKLNLSFANDGNVPYVNLLQMINSLDGFFDKNYINFSTNILFSELKITTKFGYISFNWKTNKITMPNTYYEYITKSPGKTNFNKYLKSKSYYSKDFNKNTIVIDLKKYNFDILYHNGNVLLPLAIFNLLFCSTNYYNLYFNGNKIYGCDFDIKSNVQEIPQIRNGKFQNSLIPNDIVEAKFNFTALLIDYFYGLKENNNINSFYSTISNEQKQRMLSQNPVISTKEYIKYLYNLNDLHTWVSMPSFYNRKDSSIAKDFY" }, "paralog": false, "identity": 0.9661016949152542, "score": 581.0, "evalue": 1.27e-213, "description": "Internal stop codon at 278121, 278667. Nonsense mutation around 278121, 278667.", "psc": { "uniref90_id": "UniRef90_UPI0034D962DE", "uniref50_id": "UniRef50_UPI0034D962DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D962DE", "UniRef:UniRef50_UPI0034D962DE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D962DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D962DE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20802.418900000008, "isoelectric_point": 9.074070167541507 }, "id": "DFOKDJOJIG_524", "locus": "GCA013367855_00500" }, { "type": "cds", "sequence": "contig_1", "start": 278676, "stop": 278804, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E356EC4", "UniRef:UniRef90_UPI001E356EC4" ], "nt": "ATGCCTTCATTTTATAATCACAAGGATTCTAGCATTGCAAAAGATTTCTATGAATATACCACAAATAAAAGAGAAAGAATTTATCAATCTACAATAAAATATTTAAAAAATAATAAATTAAATAATTGA", "aa": "MPSFYNHKDSSIAKDFYEYTTNKRERIYQSTIKYLKNNKLNN", "aa_hexdigest": "ff78031624260bd747f455f2684c0b03", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 278592, "stop": 278786, "observations": { "mutation": [ 278667 ], "stop": [ 278667 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E356EC4", "query_cov": 0.8571428571428571, "subject_cov": 0.5373134328358209, "identity": 0.9440000000000001, "score": 71.2, "evalue": 1.69e-15, "gene_start": 1, "gene_end": 36, "reference_start": 29, "reference_end": 64, "reference_sequence": "MLSQNPVISTKEYIKYLYNLNDLHTWVSMPSFYNRKDSSIAKDFYEYTTNKRERIYQSTRKYLKIIN" }, "paralog": false, "identity": 0.9375, "score": 124.0, "evalue": 4.29e-39, "description": "Internal stop codon at 278667. Nonsense mutation around 278667.", "psc": { "uniref90_id": "UniRef90_UPI001E356EC4", "uniref50_id": "UniRef50_UPI001E356EC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E356EC4", "UniRef:UniRef50_UPI001E356EC4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E356EC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E356EC4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5164.719000000001, "isoelectric_point": 9.573119926452637 }, "id": "DFOKDJOJIG_525", "locus": "GCA013367855_00501" }, { "type": "cds", "sequence": "contig_1", "start": 278979, "stop": 279218, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAATCAAGAAAAACCTAAGAAATATGAAAATAAAAAATATTATTGTAGATTTATCAACTAATAGTGGCGGCAATATAGGCGCCATGCAAAGGGGATTGGGTTTTATTACAGATGAACCAATATTAAACAAAACATATAATCGTTTAGATGAAGAAATAAATATTAGATCTGTTGAAGTCGATACAGATGGCGATGGAGATTACAAAAACGATGCTTATTCTGAATACAATTGA", "aa": "MKLIKKNLRNMKIKNIIVDLSTNSGGNIGAMQRGLGFITDEPILNKTYNRLDEEINIRSVEVDTDGDGDYKNDAYSEYN", "aa_hexdigest": "1efe6fbcb76a50a6008156400ba2ef52", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8954.955099999994, "isoelectric_point": 4.98173313140869 }, "id": "DFOKDJOJIG_526", "locus": "GCA013367855_00502" }, { "type": "cds", "sequence": "contig_1", "start": 279282, "stop": 279512, "strand": "+", "frame": 3, "gene": null, "product": "S41 family peptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005133DA3", "UniRef:UniRef90_UPI0005133DA3" ], "nt": "ATGAAATTGGGCAAGATAATAGGACAAAAAACAGGCGGTGGTGCGGCATCAATTTTACCTGTTGTTTTGCCAGATGGAACTTCAATAGTAATAAGTTCTAATGATTTATCCGTAACTATGAAAAATAATACAATTAAATCTATTGAAGGCGGAATAGAACCCGATATTTATTTAGAATACAAAGATTTTTATAATGACCAAAAACTATTAGAAATAATAAAAAATAAATAA", "aa": "MKLGKIIGQKTGGGAASILPVVLPDGTSIVISSNDLSVTMKNNTIKSIEGGIEPDIYLEYKDFYNDQKLLEIIKNK", "aa_hexdigest": "db607b7cb8c0fc7b1eb0fb0be93a9613", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 279180, "stop": 279512, "observations": { "mutation": [ 279216 ], "stop": [ 279216 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005133DA3", "query_cov": 1.0, "subject_cov": 0.628099173553719, "identity": 0.9740000000000001, "score": 146.0, "evalue": 6.24e-44, "gene_start": 1, "gene_end": 76, "reference_start": 46, "reference_end": 121, "reference_sequence": "MGLTPHFSTLPGDYKNDAYSEYNWYLLTGVNTFSAANTFTSIFKEMKLGKIIGQKTGGGAASILPVVLPDGTSIVISSNNLSVTMKNNTIKSIEGGIKPDIYLEYKDFYNDQKLLEIIKNK" }, "paralog": false, "identity": 0.9636363636363636, "score": 211.0, "evalue": 3.24e-72, "description": "Internal stop codon at 279216. Nonsense mutation around 279216.", "psc": { "uniref90_id": "UniRef90_UPI0005133DA3", "product": "S41 family peptidase", "uniref50_id": "UniRef50_UPI0005133DA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005133DA3", "UniRef:UniRef50_UPI0005133DA3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005133DA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005133DA3" ], "product": "S41 family peptidase" } }, "id": "DFOKDJOJIG_527", "locus": "GCA013367855_00503" }, { "type": "cds", "sequence": "contig_1", "start": 279996, "stop": 280535, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AD2D7", "UniRef:UniRef90_UPI0034A44355" ], "nt": "ATGTTTAAAGAATTTAAAAATGGAACTAATGTTTTCATTGAATATTTAACTCCAATATTGAATAGCTTTAAAGAAAAACATGAATTTTTAAAAACAAATGAAAGCAAATATAATGAATTAAAAAACATGTTAATAAAACATTTAGAATATATTTTAGATTACTTTTGCAATCTTAAATTTGATTATTTATTCGAAGGTAAAGATCCAAAACAAACACAATATGATAATAAAGGTGCAGGAAAGAAGGTATTGCTAATTTGGGGTGGATGGCCCATATTATTTGATGTAGAAAAATTTATAGAGCCAAAATATTCTGATCCATCCGGCAATGATTTAAAAAAGAAATTAAATGATATGGTAAATTCAAATAAAATAACAAAACAAGAATATGAAATTATTTTTAATGAATTTAAGGGAATAATATTGATATTTAAGCAACTAATCACAAATATTTATAGTAAAGATTTAAAAAATGTTTTAATGTCTAAACTGCCAAAAGTTTTTGGAGCAATAAATGATGCTAAAAAAAGTGCTAAATAA", "aa": "MFKEFKNGTNVFIEYLTPILNSFKEKHEFLKTNESKYNELKNMLIKHLEYILDYFCNLKFDYLFEGKDPKQTQYDNKGAGKKVLLIWGGWPILFDVEKFIEPKYSDPSGNDLKKKLNDMVNSNKITKQEYEIIFNEFKGIILIFKQLITNIYSKDLKNVLMSKLPKVFGAINDAKKSAK", "aa_hexdigest": "4de3163ca9d32bd447d703c3b9b0ce59", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 279996, "stop": 280835, "observations": { "mutation": [ 280547, 280574 ], "stop": [ 280547, 280574 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A44355", "query_cov": 1.0, "subject_cov": 0.4323671497584541, "identity": 1.0, "score": 363.0, "evalue": 7.07e-124, "gene_start": 1, "gene_end": 179, "reference_start": 236, "reference_end": 414, "reference_sequence": "MEFTKNNDFEKQSIDVKDITINDYKFNLKDSDFADIYEIEAVEYDKTLGKLTYKIKLKTKKSLLKEYSKNPIESTKSRTFEFNGFAKDISEITKNLESDVKNSNEFKKYQENVKLNIETNIEKKINEARKKQQKQAAHQNVIKAIIEQYKKFNEEIEKTLFELANKAINVFGQFNSNKESIKKLYNILINLIINTNDEMLNVLLELGINDKVSTGLRELTADWVLSKTVGAWAIKMFKEFKNGTNVFIEYLTPILNSFKEKHEFLKTNESKYNELKNMLIKHLEYILDYFCNLKFDYLFEGKDPKQTQYDNKGAGKKVLLIWGGWPILFDVEKFIEPKYSDPSGNDLKKKLNDMVNSNKITKQEYEIIFNEFKGIILIFKQLITNIYSKDLKNVLMSKLPKVFGAINDAKKSAK" }, "paralog": false, "identity": 0.989247311827957, "score": 546.0, "evalue": 2.65e-198, "description": "Internal stop codon at 280547, 280574. Nonsense mutation around 280547, 280574.", "psc": { "uniref90_id": "UniRef90_UPI0034A44355", "uniref50_id": "UniRef50_UPI00193AD2D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A44355", "UniRef:UniRef50_UPI00193AD2D7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193AD2D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AD2D7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 21073.32430000001, "isoelectric_point": 9.205263328552245 }, "id": "DFOKDJOJIG_528", "locus": "GCA013367855_00504" }, { "type": "cds", "sequence": "contig_1", "start": 280817, "stop": 282214, "strand": "-", "frame": 3, "gene": null, "product": "lipoprotein 17-related variable surface protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1CB34", "UniRef:UniRef90_UPI0005CB0F1B" ], "nt": "TTGGATACATTAGTTGAACAAGAAAACATTACATCTTTAAAAAATAATGAATATTTAACATCTAAAAAATCTTCAATGATTTCAAAAAATGACTTTGAATTAAACAAGCAAAAAAGTAATGAACTAGCAGGGTTGGAATTAAATATTTCAGAAATCAATGCCAATGCCAATGGAAAATTAGAAATAGCCTTTTCGTTATTGAATAAAACATTTAAACATGGAACAATAGAATCAAACAAAAAGACAGTTACATTCAATTTCTTAAGTGATGAAAATTACACATTTAATTTAATTAATAACTTAAGCATTTCAAAAGAATCAACTTATGTAACTGATGGTGTTTATACTAAAGCAGATTTTAAAAATAAAATTGCTAACAATAAACAAGAAATTGCTAAGTTATTAGGTATTTCTGATTTTCTAAATCAAAACTCAATTATTGAATTGAATCAAGAAAATGAAAACGCTATTGTGTCATTAAATAAATTTGTAAATACTACTACTGTAAAATATGAATTTATTTTAAAGATAAAATCAGCAAATGATAAACTTTTTAAAAAATTCTCGGGTGAAATTTCTATTGCTAATATTATGAATAAACGAAATTTAAAAAATTTTGCAAATAACTTTGATTTTGATTTTAAAGATTACACCAAAAAAGAATTAGCAAAATCAATGACAATAGATGAATCAATAGAAAAAGGCTACAATTTCTTTTTTAGAGAAAAAATTAATTTTGGTAGCGGAGAAGAATCTTATATTATTGATGATTTTAAAATTCAAAAAGATTATAAACTACTGGCAAGTGAAAATGCAAATGATATAAATATACAAATAACATTCAAATTAGACGATTATAGTGAAATAAAAACCTTTACTAAATCAGGTTTCAATGACAAAGCAAGCATTGAAAAAATAAAAGAAGAATTAAAAAATGAATCAAAAAGGCTTGACGATTTATTAGATAAACTTACAATGGAATTCACTAAAAATAATGATTTTGAAAAGCAATCTATTGATGTTAAAGATATTACAATTAATGATTATAAATTTAATCTAAAAGATAGTGATTTTGCAGATATCTATGAAATAGAGGCAGTTGAATATGATAAAACATTAGGAAAATTAACTTATAAAATTAAGTTAAAAACGAAGAAAAGTTTATTGAAAGAATATAGCAAAAATCCAATAGAGTCTACTAAATCAAGAACATTTGAATTTAATGGATTTGCTAAGGATATAAGTGAAATAACTAAAAATTTAGAATCAGATGTAAAAAAATCAGATGAATTTAAAAAATATCTAGAAAATGTTAAATTAAATATTGAAACAAATATTGAAAAAAAGATTAATGAAGCAAAAAAAAACAACAAAAACAAGCCGCTCATCAAAACGTAA", "aa": "MDTLVEQENITSLKNNEYLTSKKSSMISKNDFELNKQKSNELAGLELNISEINANANGKLEIAFSLLNKTFKHGTIESNKKTVTFNFLSDENYTFNLINNLSISKESTYVTDGVYTKADFKNKIANNKQEIAKLLGISDFLNQNSIIELNQENENAIVSLNKFVNTTTVKYEFILKIKSANDKLFKKFSGEISIANIMNKRNLKNFANNFDFDFKDYTKKELAKSMTIDESIEKGYNFFFREKINFGSGEESYIIDDFKIQKDYKLLASENANDINIQITFKLDDYSEIKTFTKSGFNDKASIEKIKEELKNESKRLDDLLDKLTMEFTKNNDFEKQSIDVKDITINDYKFNLKDSDFADIYEIEAVEYDKTLGKLTYKIKLKTKKSLLKEYSKNPIESTKSRTFEFNGFAKDISEITKNLESDVKKSDEFKKYLENVKLNIETNIEKKINEAKKNNKNKPLIKT", "aa_hexdigest": "d14dde03d6d3206cf7427b0c61554997", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 280817, "stop": 282514, "observations": { "mutation": [ 282337 ], "stop": [ 282337 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005CB0F1B", "query_cov": 0.9548387096774194, "subject_cov": 0.4983164983164983, "identity": 0.998, "score": 822.0, "evalue": 2.3e-292, "gene_start": 22, "gene_end": 465, "reference_start": 448, "reference_end": 891, "reference_sequence": "MGFNTLLNIERQETSKKLSNLVNSIDLNEFLALEKHNKKPSEIKNVDFNKKNIDGAMLVIEKMQANDEEGTLLISFYLKSTNPKFSDLKTEIKTIKLTDFLTNAQFINNQRNETIAYLNELIGNLNIKVSDKKPETAIKRFASSIKQSDLNIEIPKPNNHFKNIEVVVKQLIPNDESGKLSIRLAIKQLNKQINEYCESSKFIEVELSGYKTKTMDKNEKIESLNKVAKETTTLRLISSKNIAKTFASDVQINDFETKISNDFYIEILEISESNDDKGNLKIKFVVKTKEKQYKDLKSNEQTITLSGFYTKEQYLKACKQELNKLISDDKIEANLVFDKENDKSNILPSNVEGKEIHLNISDSKYTIEGNPSIIADDDQGILSVSWTIKVKEEKFKTFIVSESRISKKITNFLTKKQNEINIEQKRLDTLVEQENITSLKNNEYLTSKKSSMISKNDFELNKQKSNELAGLELNISEINANANGKLEIAFSLLNKTFKHGTIESNKKTVTFNFLSDENYTFNLINNLSISKESTYVTDGVYTKADFKNKIANNKQEIAKLLGISDFLNQNSIIELNQENENAIVSLNKFVNTTTVKYEFILKIKSANDKLFKKFSGEISIANIMNKRNLKNFANNFDFDFKDYTKKELAKSMTIDESIEKGYNFFFREKINFGSGEESYIIDDFKIQKDYKLLASENANDINIQITFKLDDYSEIKTFTKSGFNDKASIEKIKEELKNESKRLDDLLDKLTMEFTKNNDFEKQSIDVKDITINDYKFNLKDSDFADIYEIEAVEYDKTLGKLTYKIKLKTKKSLLKEYSKNPIESTKSRTFEFNGFAKDISEITKNLESDVKKSDEFKKYLENVKLNIETNIEKKINEAKKNNKKKPLIKT" }, "paralog": false, "identity": 0.9964601769911504, "score": 1055.0, "evalue": 0.0, "description": "Internal stop codon at 282337. Nonsense mutation around 282337.", "psc": { "uniref90_id": "UniRef90_UPI0005CB0F1B", "product": "lipoprotein 17-related variable surface protein", "uniref50_id": "UniRef50_UPI0034A1CB34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005CB0F1B", "UniRef:UniRef50_UPI0034A1CB34" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A1CB34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1CB34" ], "product": "lipoprotein 17-related variable surface protein" } }, "id": "DFOKDJOJIG_529", "locus": "GCA013367855_00505" }, { "type": "cds", "sequence": "contig_1", "start": 282335, "stop": 283324, "strand": "-", "frame": 3, "gene": null, "product": "lipoprotein 17-related variable surface protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E4E6412", "UniRef:UniRef90_UPI001E4E6412" ], "nt": "ATGTTAGTTATTGAAAAAATGCAAGCCAATGACGAAGAAGGAACATTGTTAATTAGTTTTTATTTAAAAAGTACTAATCCAAAATTCAGCGATTTAAAAACAGAAATTAAAACAATAAAATTGACAGATTTTTTAACAAATGCACAATTTATTAATAACCAAAGAAATGAAACAATTGCTTATTTAAATGAATTAATAGGAAATTTAAATATAAAAGTAAGCGATAAGAAACCTGAAACTGCTATTAAAAGATTTGCATCTTCAATCAAACAATCAGATTTAAATATTGAAATTCCAAAGCCAAATAATAATTTTAAAAATATTGAAGTTGTTGTAAAGCAATTAATTCCAAACGATGAAAGTGGAAAATTAAGTATTAGATTAGCAATCAAACAATTGAACAAACAAATTAATGAATATTGCGAATCAAGCAAATTCATTGAAGTTGAATTAAGTGGCTATAAAACTAAGACAATGGATAAAAACGAAAAAATAGAATCTTTAAATAAAGTTGCCAAAGAAACAACAACGTTAAGATTAATTTCTTCAAAAAATATTGCCAAAACATTTGCTTCTGATGTTCAAATAAACGATTTTGAAACAGAAATTAGCAATGATTTTTATATTGAAATTTTAGAAATAAGTGAATCAAATGATGATAAAGGAAGCTTAAAAATTAAATTTGTTGTTAAAACAAAAGAAAAGCAATATAAAGATTTAAAATCAAATGAACAAACTATAACATTGTCAGGTTTTTATACAAAAGAACAATACTTAAAGGCTTGCAAGCAAGAGTTGAATAAATTAATTAGCGATGATAAGATAGAAGCAAATTTAGTTTTTGATAAAGAGAATGATAAGTCAAATATATTACCTTCAAATGTTGAAGGTAAAGAAATTCATCTAAATATAAGCGATTCAAAATATACTATTGAAGGAAATCCATCAATTATTGCAGATGATGATCAAGGAATTTTGTCAGTAAGCTGA", "aa": "MLVIEKMQANDEEGTLLISFYLKSTNPKFSDLKTEIKTIKLTDFLTNAQFINNQRNETIAYLNELIGNLNIKVSDKKPETAIKRFASSIKQSDLNIEIPKPNNNFKNIEVVVKQLIPNDESGKLSIRLAIKQLNKQINEYCESSKFIEVELSGYKTKTMDKNEKIESLNKVAKETTTLRLISSKNIAKTFASDVQINDFETEISNDFYIEILEISESNDDKGSLKIKFVVKTKEKQYKDLKSNEQTITLSGFYTKEQYLKACKQELNKLISDDKIEANLVFDKENDKSNILPSNVEGKEIHLNISDSKYTIEGNPSIIADDDQGILSVS", "aa_hexdigest": "48d85dd857d81d086b0847904233743c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 282321, "stop": 283623, "observations": { "deletion": [ 282342, 283341 ], "mutation": [ 283515, 283545 ], "stop": [ 283515, 283545 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E4E6412", "query_cov": 0.993920972644377, "subject_cov": 0.41815856777493604, "identity": 0.972, "score": 594.0, "evalue": 7.2e-207, "gene_start": 1, "gene_end": 327, "reference_start": 449, "reference_end": 775, "reference_sequence": "MLLPFYLYRVISSSRNKTQDKETETTKLKEQTRNIKPNLKLSIKQDNILPSEINSSNFYVDNLDKEISFEISNIIADDVNGNVIINYYFTKSIIDSKTRQPLLIKSNMFSQKYTGFKALVAPLNSSIEQVNLEINEFNKKDFLPSQYIKLQSNFSFKNIDSQYELKTEYIANDEQGKIEVKYYYVQKDKDIKSKIISKTFYGFNSLNNIANTQKEANTLNKLGNEISLKLKKQINKSETYASTLNWNDFETSKKLNSAYLRSNGELIFDDINGEVKLNYYFEKQYIDKKTGASYWIKSKVYTTKITGFKSFLKNLNIAVDKVNLSINEKNINTILPSQYIQNLSNFKFDNLNNEYELQKKFSYDDQKGELTISYWYNDNKKNLKSNIVTKKFVGFNTLLNIERQETSKKLSNLVNSIDLNEFLAPEKHNKKPSEIKNVDFNRKNIDGAMLVIEKMQANDEEGTLLISFYLKSTNPKFSDLKTEIKTIKLTDFLTNAQFINNQRNETIAYLNELIGNLNIKVSDKKPETAIKRFASSIKQSDLNIEIPKPNNHFKNIEVVVKQLIPNDESGKLSIRLAIKQLNKQINEYCESSKFIEVELSGYKTKTMDKNEKIESLNKVAKETTTLRLISSKNIAKTFASDVQINDFETKISNDFYIEILEISESNDDKGNLKIKFVVKTNEKQYKDLRSNEQTITLTGFYTKEQYLKACKQELNKLISDDKIEANVVFDKENDKSNILPSNVEGKEIHLNISDSKYIIEGNSSIIADDDQGILSKKLNNKS" }, "paralog": false, "identity": 0.967741935483871, "score": 805.0, "evalue": 4.59e-292, "description": "Internal stop codon at 283515, 283545. Frameshift due to deletion around 282342, 283341. Nonsense mutation around 283515, 283545.", "psc": { "uniref90_id": "UniRef90_UPI001E4E6412", "product": "lipoprotein 17-related variable surface protein", "uniref50_id": "UniRef50_UPI001E4E6412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E4E6412", "UniRef:UniRef50_UPI001E4E6412" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E4E6412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E4E6412" ], "product": "lipoprotein 17-related variable surface protein" } }, "id": "DFOKDJOJIG_530", "locus": "GCA013367855_00506" }, { "type": "cds", "sequence": "contig_1", "start": 283318, "stop": 283491, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175972251.1", "SO:0001217", "UniParc:UPI001588B0E4", "UniRef:UniRef100_UPI001588B0E4", "UniRef:UniRef50_UPI001588B0E4", "UniRef:UniRef90_UPI001588B0E4" ], "nt": "GTGGGATTTAATACATTACTCAACATAGAAAGACAAGAAACATCAAAGAAACTTTCTAATTTAGTTAATTCAATTGATCTAAACGAATTTTTGGCACTTGAAAAGCACAACAAAAAACCTTCGGAAATCAAAAATGTTGATTTCAATAAAAAAATATTGATGGAGCAATGTTAG", "aa": "MGFNTLLNIERQETSKKLSNLVNSIDLNEFLALEKHNKKPSEIKNVDFNKKILMEQC", "aa_hexdigest": "bca7c62231e8c3c16d7e6d028fb9b138", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001588B0E4", "ncbi_nrp_id": "WP_175972251.1", "uniref100_id": "UniRef100_UPI001588B0E4", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588B0E4", "RefSeq:WP_175972251.1", "UniRef:UniRef100_UPI001588B0E4" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588B0E4", "uniref90_id": "UniRef90_UPI001588B0E4" }, "psc": { "uniref90_id": "UniRef90_UPI001588B0E4", "uniref50_id": "UniRef50_UPI001588B0E4" }, "pscc": { "uniref50_id": "UniRef50_UPI001588B0E4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001588B0E4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6649.6276, "isoelectric_point": 7.88121166229248 }, "id": "DFOKDJOJIG_531", "locus": "GCA013367855_00507" }, { "type": "cds", "sequence": "contig_1", "start": 283930, "stop": 284709, "strand": "-", "frame": 1, "gene": null, "product": "lipoprotein 17-related variable surface protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A2EAC", "UniRef:UniRef90_UPI00193A2EAC" ], "nt": "ATGAAAAAGAATACAAAAATCAAAAGCATTTCCATTTTAAGCGCTACTGCCATTTTGCTTGTATCAGGAGTCATTTCATCAGCCTGCAACAAGACGCAAGATAAAGAAACTGAAACAACAAAATTAAAGGAACAAACAAGAAATATTAAACCAAATTTAAAACTTTCTATTAAACAGGATAAGATACTTCCAAGTGAAATTAATTCATCAAATTTTTATGTCAATAATCTTGATAAAGAAATATCGTTTGAAATCTCTAATATTATTGCAGATGATGTAAACGGGAATGTTATTATAAATTATTATTTTACAAAATCAATAATCGATTCTAAAACAAGACAACCACTGTTAATAAAATCAAATACGTTTTCTCAAAAATATACAGGATTTAAAGCGTTGGTTGCTCCTTTAAATTCGTCAATTGAACAAGTTAATCTAGAAATAAATGAATTTAATAAAAAAGACTTTTTGCCTTCGCAATATATTAAATTGCAAAGCAATTTTTCATTTAAAAACATCGATTCCCAATATGAATTAAAAACTGAATATATTGCAAACGATGAACAAGGCAAAATAGAAGTTAAATATTATTATGTACAAAAAGACAAAGATATAAAATCAAAAATTATTTCAAAAACATTTTATGGTTTTAATTCATTGAATAATATAGCTAATACGCAAAAAGAAGCTAACACTTTAAATAAGTTAGGAAATGAGATATCTTTAAAATTAAAGAAACAAATTAATAAATCAGAAACATATGCTTCAACATTGAATTGA", "aa": "MKKNTKIKSISILSATAILLVSGVISSACNKTQDKETETTKLKEQTRNIKPNLKLSIKQDKILPSEINSSNFYVNNLDKEISFEISNIIADDVNGNVIINYYFTKSIIDSKTRQPLLIKSNTFSQKYTGFKALVAPLNSSIEQVNLEINEFNKKDFLPSQYIKLQSNFSFKNIDSQYELKTEYIANDEQGKIEVKYYYVQKDKDIKSKIISKTFYGFNSLNNIANTQKEANTLNKLGNEISLKLKKQINKSETYASTLN", "aa_hexdigest": "fbc1b6416ed00428878dfa3c43b556a0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 283627, "stop": 284709, "observations": { "mutation": [ 283785, 283932 ], "stop": [ 283785, 283932 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193A2EAC", "query_cov": 1.0, "subject_cov": 0.6675257731958762, "identity": 0.988, "score": 470.0, "evalue": 5.93e-165, "gene_start": 1, "gene_end": 259, "reference_start": 1, "reference_end": 259, "reference_sequence": "MKKNTKIKSISILSATAILLVSGVISSACNKTQDKETETTKLKEQTRNIKPNLKLSIKQDKILPSEINLSNFYVNNLDKEISFEISNIIADDVNGNVIINYYFTKSIIDSKTRQPLLIKSNTFSQKYTGFKALVAPLNSSIEQVNLEINEFNKKDFLPSQYIKLQSNFSFKNIDSQYELKTEYIANDGQGKIEVKYYYVQKDKDIKSKIISKTFYGFNSLNNIANMQKEANTLNKLGNEISLKLKKQINKSETYASTLNWSDFETSKKLNSAYLRSNGELIFDDINGEVKLNYYFEKQYIDKKTGASYWIKSKVYTTKITGFKSFLKNLNIAVDKVNLSINEKNINTILPSQYIQNLSNFKFDNLNNEYELQKKFSYDDQKGELTISY" }, "paralog": false, "identity": 0.9833333333333333, "score": 683.0, "evalue": 2.18e-251, "description": "Internal stop codon at 283785, 283932. Nonsense mutation around 283785, 283932.", "psc": { "uniref90_id": "UniRef90_UPI00193A2EAC", "product": "lipoprotein 17-related variable surface protein", "uniref50_id": "UniRef50_UPI00193A2EAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193A2EAC", "UniRef:UniRef50_UPI00193A2EAC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193A2EAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A2EAC" ], "product": "lipoprotein 17-related variable surface protein" } }, "id": "DFOKDJOJIG_532", "locus": "GCA013367855_00508" }, { "type": "cds", "sequence": "contig_1", "start": 284792, "stop": 285796, "strand": "-", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_044420562.1", "SO:0001217", "UniParc:UPI0005CA6615", "UniRef:UniRef100_UPI0005CA6615", "UniRef:UniRef50_D1J7Z7", "UniRef:UniRef90_D1J7Z7" ], "nt": "ATGAAAAAATCCAAAAAAATTTTATTAACTATGGGGGTAGCACTTCCATTATTAGCCTCAACTCCATTGGTTGCTGCGGGTTGCGTAAAAACTAAACCAGAAGTTAAACCAGAAGATCCAAAGAATCCTGAAGGTGAAAAACCAGGAACAACACCCGAAAAGAAGCCTGAAGGTGAAAAACCAGGAACAACACCAGAAGTTAAACCAGAAGAAAAAAGCGAACTTCAAAAAGCAACTGAAGGTATTGCAGATGCTACAGTTAAAGCAAATTTTGAAAGTGAATTAAAGAAATATCAAGAAGAATTAAAAACTAAATCAGAAGCCATTTTAAAGCCAATTATTGAAAATAAAGAAATTATAGCTGCAGTTTCTGAAAAAGAATTGCCAAATATTACAAAAACATATAATGAAGCAATTTCTAAATTAAATGAAATTTTAGATGATATTATTTTGTCAACACTAAAAGAATACAACACAAATAAAACAAAAGTTTCTGATGGATTAATGACAATTAAAAAATATTTAACAAAAGAAAAAATTTCTAATTTAATTAAAAAAGAAAATGGGTTGGCAGATTTCTTGGTTACATTTGGAAAAAATATTTCTAAATATGCAAAAGCAATTTTTGAAACACTAAAAACAAACGAATCAACAAAAGCAATAGCTCAATTGAACGATTTAATTACAGCTGTTAATGATAAATTTGCTGGAAAAGATAATTTATTTGAAACAACCGCTAAGAAATTAAAAGAATTTGATTTTAGTAAATTGACTGAAGAAGGATATTCAAAAACATTTGAAAAATTAATTAAATTATTATCTGATGCTAAGGCAGAAGCAGTTGCAGTATTAAAATCTGTAAAAATGTCTGATGAAGATATTAAAAAAGTAAAAGATGTAATTAGACCAATTTACAACGTTTTAAATGAAGGCCTAAAAAACTCAATTAACTTATTTGAACAATCATTGAGAGATTTACCAAAAGTGTTAGAAATCTTAAAATAA", "aa": "MKKSKKILLTMGVALPLLASTPLVAAGCVKTKPEVKPEDPKNPEGEKPGTTPEKKPEGEKPGTTPEVKPEEKSELQKATEGIADATVKANFESELKKYQEELKTKSEAILKPIIENKEIIAAVSEKELPNITKTYNEAISKLNEILDDIILSTLKEYNTNKTKVSDGLMTIKKYLTKEKISNLIKKENGLADFLVTFGKNISKYAKAIFETLKTNESTKAIAQLNDLITAVNDKFAGKDNLFETTAKKLKEFDFSKLTEEGYSKTFEKLIKLLSDAKAEAVAVLKSVKMSDEDIKKVKDVIRPIYNVLNEGLKNSINLFEQSLRDLPKVLEILK", "aa_hexdigest": "9cd574f881195c68c1e6880b969e7ac0", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0005CA6615", "ncbi_nrp_id": "WP_044420562.1", "uniref100_id": "UniRef100_UPI0005CA6615", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005CA6615", "RefSeq:WP_044420562.1", "UniRef:UniRef100_UPI0005CA6615" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005CA6615", "uniref90_id": "UniRef90_D1J7Z7" }, "psc": { "uniref90_id": "UniRef90_D1J7Z7", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J7Z7" }, "pscc": { "uniref50_id": "UniRef50_D1J7Z7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Z7" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "DFOKDJOJIG_533", "locus": "GCA013367855_00509" }, { "type": "cds", "sequence": "contig_1", "start": 286154, "stop": 286306, "strand": "+", "frame": 2, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "COG:COG0267", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_012855488.1", "SO:0001217", "UniParc:UPI0001BC21A4", "UniRef:UniRef100_D1J7Z8", "UniRef:UniRef50_P35871", "UniRef:UniRef90_B3PMG6" ], "nt": "ATGCCAAGAGAAGGTTTAACCTTAAGATGTGAAAAATGTAAAATGGAAAATTACATTACAAAAAAGAACAAAAAATTACATCCAGACAAAATGGAAGTACAAAAATACTGCTCAAAATGTAATGCACATACAACACACAAAGAAAAGAAATAA", "aa": "MPREGLTLRCEKCKMENYITKKNKKLHPDKMEVQKYCSKCNAHTTHKEKK", "aa_hexdigest": "a94740e22d2064ea07f0b499858aa7c7", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC21A4", "ncbi_nrp_id": "WP_012855488.1", "uniref100_id": "UniRef100_D1J7Z8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21A4", "RefSeq:WP_012855488.1", "UniRef:UniRef100_D1J7Z8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7Z8", "uniref90_id": "UniRef90_B3PMG6" }, "psc": { "uniref90_id": "UniRef90_B3PMG6", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P35871", "cog_id": "COG0267", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P35871", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35871" ], "product": "Large ribosomal subunit protein bL33" }, "genes": [ "rpmG" ], "id": "DFOKDJOJIG_534", "locus": "GCA013367855_00510" }, { "type": "cds", "sequence": "contig_1", "start": 286587, "stop": 286997, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00557.29" ], "nt": "TTGGCTAAAAAGAAATATGCAACTGTTGGTGTTGAAAAGGACTATTTAATACTCGAAACATTTGAAAATTTAAAATCAATGTTGCCAAAATCTAAATTTGTGTTAGTAAATGGACAAGAATTTAGAATACTAAAAGATGAAGAAGAATATAGCAATGTTCAAGAAGCATGTAACATTTCTCTTCAATCTTTTGAACAAATAAAAACCGTTCTAACTGAAGGAATTACAGAATTAGAAGTTTCAAATAAATTAGGCTACTTAATGCGTTTATTTGGAGCTGAAAAAGAATCATTTGAAACAATTGTAGCTTTTGGTACAAATGCTGCTGAACCTCACCACCATCCAACAGATCGTAAACTTGCTGATGGTGATATTGTTAAGATAGACTTTGGTGCTCAATATCAAGGTTGA", "aa": "MAKKKYATVGVEKDYLILETFENLKSMLPKSKFVLVNGQEFRILKDEEEYSNVQEACNISLQSFEQIKTVLTEGITELEVSNKLGYLMRLFGAEKESFETIVAFGTNAAEPHHHPTDRKLADGDIVKIDFGAQYQG", "aa_hexdigest": "01e59e4a40b1bf253a9a3c195050a784", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00557.29", "name": "Peptidase_M24", "length": 87, "aa_cov": 0.625, "hmm_cov": 1.0, "evalue": 2.4888004011087295e-22, "score": 75.3111572265625, "start": 52, "stop": 136 } ], "seq_stats": { "molecular_weight": 15395.32599999998, "isoelectric_point": 5.111382865905762 }, "id": "DFOKDJOJIG_535", "locus": "GCA013367855_00511" }, { "type": "cds", "sequence": "contig_1", "start": 287274, "stop": 287399, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00557.29" ], "nt": "ATGATTATTACTGTTGAACCTGGTATATATATCGAAAACGAAGGTGGCGCTAGAATAGAAGACACTATTTTGGTTACAAAAGACGGCTCAAAAGTATTAAGCAGACCCGAAGATTATGAAAAATAA", "aa": "MIITVEPGIYIENEGGARIEDTILVTKDGSKVLSRPEDYEK", "aa_hexdigest": "4b564575859134acf0642200976ffa80", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00557.29", "name": "Peptidase_M24", "length": 28, "aa_cov": 0.6585365853658537, "hmm_cov": 1.0, "evalue": 1.2540319716995536e-09, "score": 33.81732940673828, "start": 1, "stop": 27 } ], "seq_stats": { "molecular_weight": 4580.1284000000005, "isoelectric_point": 4.442785835266113 }, "id": "DFOKDJOJIG_536", "locus": "GCA013367855_00512" }, { "type": "cds", "sequence": "contig_1", "start": 287515, "stop": 287712, "strand": "+", "frame": 1, "gene": "dprA", "product": "DNA-protecting protein DprA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E573C53", "UniRef:UniRef90_UPI001E573C53" ], "nt": "ATGAACAAAGAAGAATATTTAGCTTATTTAAGTTATATAAATAATGGCAAATGGCACCAAATCTATAATGATTTAAAATCACAAATAACATGTCAAAAAACAAAATATGCTGATTTTTGTAGTGACCAAGCAATCAAAAACAAATCATACATAACAATTTTTGATAGTGATTATCCGCTATATTGCAATAATGTTTGA", "aa": "MNKEEYLAYLSYINNGKWHQIYNDLKSQITCQKTKYADFCSDQAIKNKSYITIFDSDYPLYCNNV", "aa_hexdigest": "b339db72e7c68f95f345321a0826057f", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dprA" ], "truncated": "3-prime", "pseudogene": { "start": 287515, "stop": 287935, "observations": { "insertion": [ 287908 ], "mutation": [ 287710, 287866 ], "stop": [ 287710, 287866 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E573C53", "query_cov": 1.0, "subject_cov": 0.4676258992805755, "identity": 1.0, "score": 142.0, "evalue": 1.6e-42, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MNKEEYLAYLSYINNGKWHQIYNDLKSQITCQKTKYADFCSDQAIKNKSYITIFDSDYPLYCNNVWNPPFVLYYYGNKQLLKHKIIMITGNISISNIIEVKDSIKNFGKEYVFCNEAWKGLDIQLIDYLISKIKILLLF" }, "paralog": false, "identity": 0.9856115107913669, "score": 276.0, "evalue": 1.73e-97, "description": "Internal stop codon at 287710, 287866. Frameshift due to insertion around 287908. Nonsense mutation around 287710, 287866.", "psc": { "uniref90_id": "UniRef90_UPI001E573C53", "product": "DNA-protecting protein DprA", "uniref50_id": "UniRef50_UPI001E573C53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E573C53", "UniRef:UniRef50_UPI001E573C53" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E573C53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E573C53" ], "product": "DNA-protecting protein DprA" } }, "id": "DFOKDJOJIG_537", "locus": "GCA013367855_00513" }, { "type": "cds", "sequence": "contig_1", "start": 287995, "stop": 288273, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTATTCATTTCTGAATACCCTAGCAATTATCACACAAGTAAAATAACGCTAAAAGCGCGAAATAGAATTAGCGCAGCAATTTCTAATTATTTAGTCTTACTCTCTTGTTATAAAAGCACCATGAATAACCTAATAGATCAATTCTTAAATATTGGCAAAGAGGTTTATTGCCTTGATGATATTCAAAATAATTTGAATGAAGATAATTTCAACAAAAACTTAATTAATGAAGGAGCAGGAAAAATAACTTCAATAACTGATTTAATATTAAAATAG", "aa": "MLFISEYPSNYHTSKITLKARNRISAAISNYLVLLSCYKSTMNNLIDQFLNIGKEVYCLDDIQNNLNEDNFNKNLINEGAGKITSITDLILK", "aa_hexdigest": "833c2b3ed8d3b2711a41fee5a7c0f4ef", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10477.887899999987, "isoelectric_point": 6.536677360534668 }, "id": "DFOKDJOJIG_538", "locus": "GCA013367855_00514" }, { "type": "cds", "sequence": "contig_1", "start": 288265, "stop": 288510, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01261.30" ], "nt": "TTGCCTTTAATTAGAGTTATTCATTTAAATGATTCCATGAATGAACTTGGATCAAAAAAAGATCGTCATGCAAACATTGATAGAGGCCATATAGGTTTAGAAACATTGAAAAAATTTGTTCATGATAAAGACTTCGATAATATTCCCATTATTTTAGAAACACCGTTTATTAATAATAAAAGTCCTTACAAAGATGAAATAATAATGCTTTTAGAGGATAATAAAAAAGATAATATTCTATTTTAA", "aa": "MPLIRVIHLNDSMNELGSKKDRHANIDRGHIGLETLKKFVHDKDFDNIPIILETPFINNKSPYKDEIIMLLEDNKKDNILF", "aa_hexdigest": "e290778ae957a7b63e5fde107fb39237", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387AC6AF", "query_cov": 0.8641975308641975, "subject_cov": 1.0144927536231885, "identity": 0.7709999999999999, "score": 105.0, "evalue": 3.43e-28, "valid": false, "product": "TIM barrel protein", "uniref50_id": "UniRef50_Q49058" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01261.30", "name": "AP_endonuc_2", "length": 67, "aa_cov": 0.8148148148148148, "hmm_cov": 0.9850746268656716, "evalue": 1.526081964509264e-14, "score": 49.893653869628906, "start": 4, "stop": 69 } ], "seq_stats": { "molecular_weight": 9483.860399999992, "isoelectric_point": 6.0339365005493155 }, "id": "DFOKDJOJIG_539", "locus": "GCA013367855_00515" }, { "type": "cds", "sequence": "contig_1", "start": 288574, "stop": 289116, "strand": "-", "frame": 1, "gene": "nfo", "product": "deoxyribonuclease IV", "db_xrefs": [ "COG:COG0648", "COG:L", "EC:3.1.21.2", "GO:0003677", "GO:0006281", "GO:0008270", "SO:0001217", "UniRef:UniRef50_Q4A6S5", "UniRef:UniRef90_D1J801" ], "nt": "ATGATTAAATTAGGAAGCCATATATCATTTAAAAAACCCGATTATTTAATAGGTTCATTTAATGAAGCTATTAATAATGGCGAGAATTGTTTTATGATTTACTTAGGAGCACCTCAAAGTGCTTTTAGGGTTGATAGTTCAATGTATAATATTGAAAAATATTTAAAGCAATGCAATAGTATTATTCCTGTATCTGATATTGTGGTTCATGCCCCATATATAGTAAATTTAGCCAATAGAGAAAAGGCAGATTTTGGGATTAAATTTTTATGTAGTGAAATTAAAAGAATGAATAGTTTTGGTGCTAAATTGTTAGTTTTACACCCTGGTTCATCAGTTGAATATAGCAGAAGCGAAGCATTAGCATCATTAATTCAATCTCTTAAAAAGATAATTGAATCTACTAAGGACGTAATAATATGCCTTGAAATAATGTCGGGTAAAGGTAGTCAAATTTGTAGCAATTTCAATGACTTATTATACGTGATAAAATCCGTTAATTCCGAAAGAATTGCAATATGTTTTGATACTTGCCATGCCTGA", "aa": "MIKLGSHISFKKPDYLIGSFNEAINNGENCFMIYLGAPQSAFRVDSSMYNIEKYLKQCNSIIPVSDIVVHAPYIVNLANREKADFGIKFLCSEIKRMNSFGAKLLVLHPGSSVEYSRSEALASLIQSLKKIIESTKDVIICLEIMSGKGSQICSNFNDLLYVIKSVNSERIAICFDTCHA", "aa_hexdigest": "7f9b1686e745e748bbc8f1126fd906cc", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "nfo" ], "truncated": "5-prime", "pseudogene": { "start": 288271, "stop": 289116, "observations": { "mutation": [ 288576 ], "stop": [ 288576 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J801", "query_cov": 1.0, "subject_cov": 0.6360424028268551, "identity": 0.983, "score": 353.0, "evalue": 8.03e-122, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MIKLGSHISFKKPDYLIGSFNEAINNGENCFMIYLGAPQSAFRVDSSLYNIEKYLKQCNSIIPVSDIVVHAPYIVNLANREKVDFGIKFLCSEIKRMNSFGAKLLVLHPGSSVEYSRSEALASLIQSLKKIIESTKDVIICLEIMSGKGSQICSNFNDLLYVIKSVNSERIAICLDTCHAWDAGYDIKDYENFKSYIKQQNILPLIRVIHLNDSMNELGSKKDRHANIDKGHIGLETLKKFVHDKDFDNIPIILETPFINNKNPYKDEIIMLLEDNKKDNILF" }, "paralog": false, "identity": 0.9786476868327402, "score": 553.0, "evalue": 5.99e-203, "description": "Internal stop codon at 288576. Nonsense mutation around 288576.", "psc": { "uniref90_id": "UniRef90_D1J801", "gene": "Nfo", "product": "deoxyribonuclease IV", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_Q4A6S5", "cog_id": "COG0648", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J801", "EC:3.1.21.2", "UniRef:UniRef50_Q4A6S5", "COG:COG0648", "COG:L", "GO:0003677", "GO:0006281", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6S5" ], "product": "Probable endonuclease 4" } }, "id": "DFOKDJOJIG_540", "locus": "GCA013367855_00516" }, { "type": "cds", "sequence": "contig_1", "start": 289284, "stop": 289805, "strand": "-", "frame": 2, "gene": null, "product": "RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ5" ], "nt": "ATGGTTGAAACAATAAATAAAATATTGAAGATTGAACGTGAATTGCAACAAGAAAATGGATATCCTCCAACAGATGAAGAAATTGCTAAAAAATACGGTGGAGATTTTACTGCTGAAAAAGTAAGATATATTAGAAAAATTAATATTGATCCAATTAGTTTGGATAAAAACATTGGAAAAGAAGAAAATTCTAGTTTCTCAGATTTTGTTAAAGATGAAAGCGTAATCAGCCCAACTTCATATGCAAGCCATGAAGAGTTAAGTGTTATTATAAATGAAATGATTGAAGAAGGGTTAAGTGATCAAGCTGATCGGGATTTAATTAGAAAACGTTTTGGAATTAACGACTTAAAGGGAAACAAATACAAACCTCATACTTTAGATGAACTTTCAAAAGAATTAGGCATTTCGAAAGAAAAAGTTAGACAATTAGAAACAAAAGTTTTGCGTAAATTGAAGCATCCTCAAAAACGTAGAAAATTGAAGGAATTCTTTAATAGCGATAGCTATGATCTAGACTAA", "aa": "MVETINKILKIERELQQENGYPPTDEEIAKKYGGDFTAEKVRYIRKINIDPISLDKNIGKEENSSFSDFVKDESVISPTSYASHEELSVIINEMIEEGLSDQADRDLIRKRFGINDLKGNKYKPHTLDELSKELGISKEKVRQLETKVLRKLKHPQKRRKLKEFFNSDSYDLD", "aa_hexdigest": "bb6960937ffe99faca464418640fb8db", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0024C90298", "query_cov": 0.9884393063583815, "subject_cov": 0.8142857142857143, "identity": 0.561, "score": 166.0, "evalue": 3.83e-49, "valid": false, "product": "sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0A448ZYJ5" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYJ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ5" ], "product": "RNA polymerase sigma factor" }, "genes": [], "id": "DFOKDJOJIG_541", "locus": "GCA013367855_00517" }, { "type": "cds", "sequence": "contig_1", "start": 289872, "stop": 290132, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04542.20" ], "nt": "TTGCTAACTACAAATGAAGAACAAGCGCTAGCAAAAAAAATGCAAGAAGCTAAAGAAGCAAATAATTATTATAAATATAAAAAAGCTAGAGATATGTTAGTCAAAAGAAATCTCCGCTTAGTAATCAATAATGCAAAGCGTTATAAAAATCGTGGATTGAATTTTATTGATTTGATATCAGAAGGAAATGCCGGAATAATGAAGGCAGTTTCAAAATATGATTACACAAAAGGATTTAAATTTTCAACATATGCTACTTGA", "aa": "MLTTNEEQALAKKMQEAKEANNYYKYKKARDMLVKRNLRLVINNAKRYKNRGLNFIDLISEGNAGIMKAVSKYDYTKGFKFSTYAT", "aa_hexdigest": "09424a3a1800bf47eb30217db6f002da", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04542.20", "name": "Sigma70_r2", "length": 54, "aa_cov": 0.627906976744186, "hmm_cov": 1.0, "evalue": 7.678067599874571e-16, "score": 53.37271499633789, "start": 33, "stop": 86 } ], "seq_stats": { "molecular_weight": 10024.499799999994, "isoelectric_point": 9.909451484680176 }, "id": "DFOKDJOJIG_542", "locus": "GCA013367855_00518" }, { "type": "cds", "sequence": "contig_1", "start": 290163, "stop": 290900, "strand": "-", "frame": 2, "gene": "rpoD", "product": "RNA polymerase sigma factor", "db_xrefs": [ "COG:COG0568", "COG:K", "SO:0001217", "UniRef:UniRef50_A0A501X9X8", "UniRef:UniRef90_D1J802" ], "nt": "ATGCAAGATACTAAAAATAATGTAGAATCATCAGTTAAGTGTATAACTAAAATTGTTGCAGACGAGTTAAAAAAAGTCCAAAAGAAAAATAAGGATAAAAAGGCTTTTACTCAAGATGAAATTTTTGAATTCCTAAACAAAAAGAACCTTTACGTTGATGACGAAGAAGCAGAAGCTTTACTTGAAAGCCTTTTTGCAGCTAATCTAGTCTCATCTGATACTGATGACGAAGATATTGAAGATATTGACATAAATTCGCTAGATGATGAAGTTGCTCACGCAACAACTAAAGATGATGATAATGATGACGATGACGCAGAAGAGGAAGAAAAAGATTTTGATCCTTCAAAAATTAAAGAGTTGGAAGAAGAAAATCTTGACGAAGATATAAATATTGATCAAGAATTTGACGATAGTTCAATTAGAAGCAAGGAAGAAGATGACTCAGAAATGTCTTTTGACGAAGATGATAATCATTTTGTTAAAAAGGCATTTAATGATCTTGATGATGAAGAATATAATGATTATTACGGCGAAGATTTTGATGAATATGAAGATGACGATTATCAAACAAACCACAAGCAAGGTAAAAAACAATCATACGATGACTTCGATGCTAACAATTTGCTAAAAGAATACAATGATGAAGATATAAAGTTGAGTGAAGAAAAAGTTTATGATCAAGATGATATAAAATTAACTAATAAGCTAACCGAAACCAACGACATTGTTAAATGA", "aa": "MQDTKNNVESSVKCITKIVADELKKVQKKNKDKKAFTQDEIFEFLNKKNLYVDDEEAEALLESLFAANLVSSDTDDEDIEDIDINSLDDEVAHATTKDDDNDDDDAEEEEKDFDPSKIKELEEENLDEDINIDQEFDDSSIRSKEEDDSEMSFDEDDNHFVKKAFNDLDDEEYNDYYGEDFDEYEDDDYQTNHKQGKKQSYDDFDANNLLKEYNDEDIKLSEEKVYDQDDIKLTNKLTETNDIVK", "aa_hexdigest": "915da4e6aba3dd5b9385155b135b4b26", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "rpoD" ], "truncated": "5-prime", "pseudogene": { "start": 289860, "stop": 290900, "observations": { "mutation": [ 289871, 289874, 290153, 290165 ], "stop": [ 289871, 289874, 290153, 290165 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J802", "query_cov": 1.0163265306122449, "subject_cov": 0.45940959409594095, "identity": 0.94, "score": 372.0, "evalue": 2.62e-124, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 249, "reference_sequence": "MQDTKQDTKNNVESSVKCITKIVADELKKVQKKNKDKKAFTQDEIFEFLNKKNLYVDDEEAEALLESLFAANLVSSDTDDEDIEDIDINSLDDEVAHATTKDDDNDDDDAEEEEKDFDPSKIKELEEENLDEDINIDQEFDDSSIRSKEEDDSEMSFDEDDNHFVKKAFNDLDDEEYNDYYGEDFDEYEDDDYQTNHKQGKKQSYDDFDANNLLKEYNDEDIKLSEEKVYDQDDIKLTNKLTETNDIVKWYMRWIGKYGKLLTTNEEQALAKKMQEAKEANNYYKYKKARDMLVKRNLRLVINNAKRYKNRGLNFIDLISEGNAGIMKAVSKYDYTKGFKFSTYATWWIRQAITRAVADQARTVRVPVHMVETINKILKIERELQQENGYPPTDEEIAKKYGGDFTAEKVRYIRKINIDPISLDKNIGKEENSSFSDFVKDESVISPTSYASHEELSVIINEMIEEGLSDQADRDLIRKRFGINDLKGNKYKPHTLDELSKELGISKEKVRQLETKVLRKLKHPQKRRKLKEFFNSDSYDLD" }, "paralog": false, "identity": 0.9571428571428572, "score": 589.0, "evalue": 4.25e-212, "description": "Internal stop codon at 289871, 289874, 290153, 290165. Nonsense mutation around 289871, 289874, 290153, 290165.", "psc": { "uniref90_id": "UniRef90_D1J802", "gene": "RpoD", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0A501X9X8", "cog_id": "COG0568", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J802", "UniRef:UniRef50_A0A501X9X8", "COG:COG0568", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501X9X8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X9X8" ], "product": "RNA polymerase sigma factor" } }, "id": "DFOKDJOJIG_543", "locus": "GCA013367855_00519" }, { "type": "cds", "sequence": "contig_1", "start": 290960, "stop": 291157, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E305FB3" ], "nt": "ATGTTTTTGTGTTTTTTAATAAACAGCATAAAAAATAATAAATCAATAGATGAAACAAATTTTGAATCATATATTACAAACAATATGGATCTTGAAGATAATGCTAAACAAATATATTTAGATTTATTCAAGGAAATTATGAGTCTTAAAAACAAAGAATATACAAAAGAAGAACTAGATAAAAATTTAAATAATTGA", "aa": "MFLCFLINSIKNNKSIDETNFESYITNNMDLEDNAKQIYLDLFKEIMSLKNKEYTKEELDKNLNN", "aa_hexdigest": "9987006a0970011617cd5afacf87670a", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI001E305FB3", "query_cov": 0.8, "subject_cov": 0.9454545454545454, "identity": 0.885, "score": 85.5, "evalue": 7.51e-21, "valid": false, "uniref50_id": "UniRef50_UPI001E305FB3" }, "pscc": { "uniref50_id": "UniRef50_UPI001E305FB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E305FB3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7787.7373999999945, "isoelectric_point": 4.613018608093261 }, "id": "DFOKDJOJIG_544", "locus": "GCA013367855_00520" }, { "type": "cds", "sequence": "contig_1", "start": 291503, "stop": 292762, "strand": "-", "frame": 3, "gene": "dnaG", "product": "DNA primase", "db_xrefs": [ "COG:COG0358", "COG:L", "EC:2.7.7.101", "KEGG:K02316", "SO:0001217", "UniRef:UniRef50_D1J803", "UniRef:UniRef90_D1J803" ], "nt": "ATGGCAGAAGATAATATATTTGAAATTGTTAAACAAAAGTTAAACATTGTTGATGTTATTTCTTTATATATTTCTTTGACAAAAAAAGGAAAGAATTATGTGGCTTGTTGCCCATTTCATGGCGAAAAAACTCCTTCTTTTGTTGTGTCACAAGAAAAACAAATATTTAAATGTTTTGGTTGCTCTAAAAGCGGAAATTTAATAACTTTCATTGAACTTTATACTAAAAAAAGCCCTCTTGAAAGTATCAAGGAGCTTGTTTCAAAATTCAATTTAAATATCGATTTAACAAGGTTTAGTAATAGTATAACTACCACAACAAAAGAACAAGAAGATTTATACAATATCAATCAAATAGCGAGCGATATATTTCAATTTGAAATTTTAGAAATTAATCAAAATGAGCAATTAAAATTGTTCCTTAAAAAAAGAAGGTTAACAAAACCACTTATTAAGGAATTTGAAATTGGCTTTGCTGATAAAGATAAAAATTTATGTAATATGCTAATTGCTAAAAATTGTTCATCATTTAATTTAGCCAATTCATCATTGATAGCAAGCAACAATTTTGATAACTTTTTTAATGATAGATTGATGTTTCCAATCAAAAATAAGGATGGAAGAATTGTTGCTTTTTCTGGAAGAAGTATTTATAACAATGTTGAACCAAAATATTTAAATAGTTCTGAAACAATAGTTTTTAAAAAGCATGAAGTTATGTTTAATTACTATCATGCATTGGATGAAATTATAAAGAAATCAAGTGTTTATTTAGTTGAGGGGCAATTTGATTGCATTGCTCTTTATAAAATAAACATAAAAAATGCAGTGGCACTTTTAGGCACAACTTTATCTATACAAAATTTAGAATCTTTAAGAAATTGTAATATTGTTTTGTTTTTTGATAATGATTTTGCCGGCAAAAATGCTACTAAGAAAAATTTAAGAATAATATTAGCAAATCAAAAAAGATATAATTTAAAAGTTTCTTTTGTTTTGAATGAACATAGCAAAGATCCTGACGAATTATATAATATTGACGAAGGAAAAACATTGTCTGAGATAGCATGTTGTCCTGTCGATTTATTTATGTATTTATTCAATGAATTTAAACAAGTATTTCAATTGGAAACCAACGAATCAAGAAAGGTTGAATTAAATGCTGAATTGTTTGAATTTGTGATTTATTTGCAAGAACCATTAATATTGAAATTAAAAGAAAAATTGATTGAAAATAAAATTTTTTCAATTGAAACTTGA", "aa": "MAEDNIFEIVKQKLNIVDVISLYISLTKKGKNYVACCPFHGEKTPSFVVSQEKQIFKCFGCSKSGNLITFIELYTKKSPLESIKELVSKFNLNIDLTRFSNSITTTTKEQEDLYNINQIASDIFQFEILEINQNEQLKLFLKKRRLTKPLIKEFEIGFADKDKNLCNMLIAKNCSSFNLANSSLIASNNFDNFFNDRLMFPIKNKDGRIVAFSGRSIYNNVEPKYLNSSETIVFKKHEVMFNYYHALDEIIKKSSVYLVEGQFDCIALYKINIKNAVALLGTTLSIQNLESLRNCNIVLFFDNDFAGKNATKKNLRIILANQKRYNLKVSFVLNEHSKDPDELYNIDEGKTLSEIACCPVDLFMYLFNEFKQVFQLETNESRKVELNAELFEFVIYLQEPLILKLKEKLIENKIFSIET", "aa_hexdigest": "1c714a4096f8c08ae7ec107ac16cbb91", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dnaG" ], "truncated": "5-prime", "pseudogene": { "start": 291203, "stop": 292762, "observations": { "mutation": [ 291505 ], "stop": [ 291505 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J803", "query_cov": 1.0, "subject_cov": 0.6768982229402262, "identity": 0.993, "score": 810.0, "evalue": 6.39e-293, "gene_start": 1, "gene_end": 419, "reference_start": 1, "reference_end": 419, "reference_sequence": "MAEDNIFEIVKQKLNIVDVISLYISLTKKGKNYVACCPFHGEKTPSFVVSQEKQIFKCFGCSKSGNLITFIELYTKKSPLESIKELVSKFNLNIDLTRFSNSITTTTKEQEDLYNINQIANDIFQFEILEINQNEQLKLFLKKRRLTKPLIKEFEIGFADKDKNLCNMLIAKNCSSFNLANSSLIASNNFDNFFNDRLMFPIKNKDGRIVAFSGRSIYNNVEPKYLNSSETIVFKKHEVMFNYYHALDEIIKKSSVYLVEGQFDCIALYKINIKNAVALLGTALSIQNLESLRNCNIVLFFDNDFAGKNATKKNLRIILANQKRYNLKVSFVLNEHSKDPDELYNIDEGKTLSEIACCPVDLFMYLFNEFKQVFQLETNESRKVELNAELFEFVIYLQEPLILKLKEKLIENKIFSVETWNKYLFLYGKENFPLHPISKNNLKPSLDNEYNKKNYSKNSNYKKYEDLNFAYSDPSFDRLDFFEEPLIQQPKLITGRNLVFYLIIKTIIKHPQYIANYQLVDFARMAFSNGCQNHKMFLCFLINSIKNNKSIDETNFESYITNNIDLEDNTKQIYLDLFKEIMSLKNKEYTKEELDKNLNNWMKETGQSKDLIINLDRRK" }, "paralog": false, "identity": 0.9903660886319846, "score": 1027.0, "evalue": 0.0, "description": "Internal stop codon at 291505. Nonsense mutation around 291505.", "psc": { "uniref90_id": "UniRef90_D1J803", "gene": "DnaG", "product": "DNA primase", "ec_ids": [ "2.7.7.101" ], "uniref50_id": "UniRef50_D1J803", "cog_id": "COG0358", "cog_category": "L", "kegg_orthology_id": "K02316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J803", "EC:2.7.7.101", "UniRef:UniRef50_D1J803", "COG:COG0358", "COG:L", "KEGG:K02316" ] }, "pscc": { "uniref50_id": "UniRef50_D1J803", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J803" ], "product": "DNA primase" } }, "id": "DFOKDJOJIG_545", "locus": "GCA013367855_00521" }, { "type": "cds", "sequence": "contig_1", "start": 292773, "stop": 293291, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "TTGCTTGGTATTGCAAATCGTGGCAATTATGATTTAAGCCAACATATGAAATATTCAAATGAATCTCTTGAATATTTGTTAGACGATGGAACTAAAATAATTCCTCATGTTATTGAACCTTCAATTGGTTTAGATCGTTTATTGCTTGCGTTGCTTGTAGATTCATATGAAGAACAAGAACTAGAAAATAATGATGTAAGAATTGTTTTAAAACTTGATTTCAATATTGCACCATATCAAGTTGCGATTTTTCCATTAATGAAAAAACTTAACTCTAAGGCTAAAGAAATATTTGATAATTTATTGCAAAAAACTGAATTAAGATTAATTTATGATGAAAGCGGTTCGATAGGAAAACGTTACCGTCGTCAAGATGCAATTGGTACACCATTTGCAGTAACAATAGATTTTGACACATTAGAAAAAAATACAGTAACTGTCAGAGAACGCGATTCTATGAAACAACATGTAGTTGAATTAGACAACTTAATTAAGTTTTTTGAAAATTATAAAAAATAA", "aa": "MLGIANRGNYDLSQHMKYSNESLEYLLDDGTKIIPHVIEPSIGLDRLLLALLVDSYEEQELENNDVRIVLKLDFNIAPYQVAIFPLMKKLNSKAKEIFDNLLQKTELRLIYDESGSIGKRYRRQDAIGTPFAVTIDFDTLEKNTVTVRERDSMKQHVVELDNLIKFFENYKK", "aa_hexdigest": "2442d64f90482f18560c9191389c5df0", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0P9T9", "query_cov": 0.9651162790697675, "subject_cov": 0.9707602339181286, "identity": 0.657, "score": 228.0, "evalue": 7.7e-74, "valid": false, "product": "Glycine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0P9T9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 93, "aa_cov": 0.5232558139534884, "hmm_cov": 1.0, "evalue": 1.4635684990494176e-23, "score": 78.49624633789062, "start": 80, "stop": 169 } ], "seq_stats": { "molecular_weight": 19984.670899999994, "isoelectric_point": 5.293097114562988 }, "id": "DFOKDJOJIG_546", "locus": "GCA013367855_00522" }, { "type": "cds", "sequence": "contig_1", "start": 293436, "stop": 293702, "strand": "-", "frame": 2, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "nt": "ATGTTTAAAACCCAAATGGGTGCAATTGATACAGCAGCAAATCAAACATATTTGCGTCCTGAAACAGCCCAAGGAATTTTTATAAATTTTAAAAATGTCTTAAGAACAACTAGAGCTAAGCTACCTTTTGGAATTGGCCAAATTGGTAAAAGTTTTAGAAATGAAATTACACCTAGGGATTTTATTTTTAGAACAAGGGAATTCGAACAAATGGAATTAGAATATTTCTGCAATCCTAGTGAAGATGAAAAGTTTTACCATTATTGA", "aa": "MFKTQMGAIDTAANQTYLRPETAQGIFINFKNVLRTTRAKLPFGIGQIGKSFRNEITPRDFIFRTREFEQMELEYFCNPSEDEKFYHY", "aa_hexdigest": "fb21550e42e24642fc3daa802e4e2712", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003241E146", "query_cov": 0.9318181818181818, "subject_cov": 0.8913043478260869, "identity": 0.695, "score": 123.0, "evalue": 5.97e-35, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_X1KVU4" }, "pscc": { "uniref50_id": "UniRef50_X1KVU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_547", "locus": "GCA013367855_00523" }, { "type": "cds", "sequence": "contig_1", "start": 293991, "stop": 294149, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGATAAAATACAAAAAAATATTCAAAATATAATAAATCACTTAAAAGTTTCTGGTTTTGTTTATCAAAATTCAGAAATCTATGGAGGCTTAATAAACACCTGGGATTATGGGCCACTAGCAGTTAATATTATGACTGCGATTAAGAAATATTGA", "aa": "MGDKIQKNIQNIINHLKVSGFVYQNSEIYGGLINTWDYGPLAVNIMTAIKKY", "aa_hexdigest": "060c8f592c19fc45fda4a41c098dc6d2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5915.793100000001, "isoelectric_point": 9.10114688873291 }, "id": "DFOKDJOJIG_548", "locus": "GCA013367855_00524" }, { "type": "cds", "sequence": "contig_1", "start": 294294, "stop": 295622, "strand": "+", "frame": 3, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "COG:COG0541", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501", "KEGG:K03106", "RefSeq:WP_175972252.1", "SO:0001217", "UniParc:UPI00158957CE", "UniRef:UniRef100_UPI00158957CE", "UniRef:UniRef50_A0A448ZXZ7", "UniRef:UniRef90_D1J805" ], "nt": "ATGTTAGATTTTATTCAAAAAAGAATGCAAAATTCAATACAAAAAATTAATAAAAAAATGTCTATTAATGAAGAAGACATTTTAGAAATTTTAAGAGAAATTAAAATATCGTTATTGGAGGCAGACGTTAACCTTGAAGTTGTTAAAAACTTCACTAAAGAAGTAAAGGCAAAAGCCCTTTCTTCTCAAATTATTGGAAAGTTAAATCAGCAACAAACAGTCCTAAAAATATTCAAAGATGAATTGACTAGAATTTTAGGTTCGAAATCAATTGATATTAAAACAAACAATAACCCTACTAAAATAATGATGGTAGGATTGCAAGGTTCAGGTAAAACTACTACTGCTGCAAAATTGTCTGTTTATTTCAAAAAGAAGAATTTGGCAGTAAACCCTTTGCTTGTTGGTGATGATATTTATAGACCTGCTGCCAGAGAACAATTGCAACAATTAGCAAAACAAACTCAAACTGATTTCTTTACTATGGAAGAAAACAATGCAATTAATATTGCTCAAAAAGCAATAGAATTGGCAAAGGAAAACAAAAACGATTTAGTAATAATCGATACTGCCGGAAGATTGGCAATTGATGAAAATTTGATGCAAGAACTAAAAGATATTAAAAAATATACTCACCCTGATTATATATTTCTAGTAGTTGATGCAATGAGCGGACAAGATGTTATAAATACAGCAAAAATTTTCCACCAAGAACTTAATTTAGATGGCACTATTATCACAAAATTAGATAGTGATGCTCGTGGTGGCGCAGCCTTGTCGATTACTCATTTATTGAACGTTCCAATAATTTTTATTGGTACTGGTGAAAAATTAACTGGTTTAGAATTATTTCATCCAGATAGAATGGCAGATAGAATATTGGGCATTGGTGATGTTCTAACATTGATTGAAAAAGCTTCGGAAGAAGTTGATGAAAAAATGATGAAAAAAATTGGCTATAAAATGCTTTCTGGCCAATTCAATTTAATGGATTTGATGAATAGTTTAGCTCAAATTAAAAAACTAGGTAAGATGAAAGCAATCTTAAGAATGATTCCGGGAATGGCTGATAAGGTTTCAGACGAAAAGATTGAAGAAGCAGAAAATAAATTTAGAATTTATACTTATTTAATTAATTCAATGACGAACTTTGAGAAAAAAAACCCACGAGTTTTAAAAAACCCAAGTAGAAAGCAAAGAATTTTGCAGGGTTCTGGTAGAAGCCCAAGAGAATTTAATATGCTAATTTCGGATTTCGAAAGAATGTCTAAGCAAATGAAAGAATTAGCAAACGGAAATCTAAAATTAAAAAATGGCTTGGATGAATAA", "aa": "MLDFIQKRMQNSIQKINKKMSINEEDILEILREIKISLLEADVNLEVVKNFTKEVKAKALSSQIIGKLNQQQTVLKIFKDELTRILGSKSIDIKTNNNPTKIMMVGLQGSGKTTTAAKLSVYFKKKNLAVNPLLVGDDIYRPAAREQLQQLAKQTQTDFFTMEENNAINIAQKAIELAKENKNDLVIIDTAGRLAIDENLMQELKDIKKYTHPDYIFLVVDAMSGQDVINTAKIFHQELNLDGTIITKLDSDARGGAALSITHLLNVPIIFIGTGEKLTGLELFHPDRMADRILGIGDVLTLIEKASEEVDEKMMKKIGYKMLSGQFNLMDLMNSLAQIKKLGKMKAILRMIPGMADKVSDEKIEEAENKFRIYTYLINSMTNFEKKNPRVLKNPSRKQRILQGSGRSPREFNMLISDFERMSKQMKELANGNLKLKNGLDE", "aa_hexdigest": "13cbb489e7fbf22360900113d09996db", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158957CE", "ncbi_nrp_id": "WP_175972252.1", "uniref100_id": "UniRef100_UPI00158957CE", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158957CE", "RefSeq:WP_175972252.1", "UniRef:UniRef100_UPI00158957CE" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158957CE", "uniref90_id": "UniRef90_D1J805" }, "psc": { "uniref90_id": "UniRef90_D1J805", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_A0A448ZXZ7", "cog_id": "COG0541", "cog_category": "U", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXZ7" ], "product": "Signal recognition particle protein" }, "genes": [ "ffh" ], "id": "DFOKDJOJIG_549", "locus": "GCA013367855_00525" }, { "type": "cds", "sequence": "contig_1", "start": 295744, "stop": 296079, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAACAATCAATTTCATTTTAAATACTTATTTTTCAAAACAAAATTGTCAAGAATTAAAACCTCGCGATAAAAAAATAGCCTCTGAAAATATCAAATATATTTTAGAAACATTTAAAAACCATTCATATTTTTTAAATAATTTATTTAATCACTTCATCAATAAAATTAAAACTATTTCTCCATATAACCTAATTGTAAAAACTAAAAAATTAAATTCAATATTTAAATTAAGAAATATTATTATTTTTGATAGATATGTTTTATCAAGTCTAAAAATATCAAAAGCAATATTAGTCTTAATTTCAATTAAAAATAAGAATAAATTATAA", "aa": "MQETINFILNTYFSKQNCQELKPRDKKIASENIKYILETFKNHSYFLNNLFNHFINKIKTISPYNLIVKTKKLNSIFKLRNIIIFDRYVLSSLKISKAILVLISIKNKNKL", "aa_hexdigest": "ec6edd12996e898096a0190c5c3803b2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13213.552599999974, "isoelectric_point": 10.082484626770018 }, "id": "DFOKDJOJIG_550", "locus": "GCA013367855_00526" }, { "type": "cds", "sequence": "contig_1", "start": 296161, "stop": 296319, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTATTTTCGTTCAACGATAATCAAAAATATATAGAAATTAAGAAATTTTTGTCTCAATTGAATACATTTAAGGAATTTATTTATTTTATAGTAGAAACACTTTTAAACTATAGCGATATTTATGCAAAATTAAAAACCTTTGATGAATTATGA", "aa": "MFLFSFNDNQKYIEIKKFLSQLNTFKEFIYFIVETLLNYSDIYAKLKTFDEL", "aa_hexdigest": "bf67931aa5b4ac998666b296d549052f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6415.358999999999, "isoelectric_point": 5.0959794998168935 }, "id": "DFOKDJOJIG_551", "locus": "GCA013367855_00527" }, { "type": "cds", "sequence": "contig_1", "start": 296464, "stop": 296628, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATGCATTTTTAAGCAACGAAACAAATAGCAAAGCTATATTTTTAACATTATTTAAAGATGGAAAAATTGATGATATATTATTTAGATTACAATCAAGACTTGAAGATGTATTTTTAACTAAAAAAACAATTATTAATTTTGATTTGCGTGAACAATGA", "aa": "MIDAFLSNETNSKAIFLTLFKDGKIDDILFRLQSRLEDVFLTKKTIINFDLREQ", "aa_hexdigest": "95c5dd58ad2fb7a5d4869a552ea8e061", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6366.297299999998, "isoelectric_point": 5.125876808166503 }, "id": "DFOKDJOJIG_552", "locus": "GCA013367855_00528" }, { "type": "cds", "sequence": "contig_1", "start": 296905, "stop": 297045, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGAAAACTTATTTAGTTGGTTTTGCACCGAAAAAGGATATAAAAAAGTTGCATATGATTTACTTGCTAAATTAAGTTTAACTAGTGAATTTAAAATGTCTAATTTAATTCAATATTGTAGTGATTCTAAATATTGA", "aa": "MYENLFSWFCTEKGYKKVAYDLLAKLSLTSEFKMSNLIQYCSDSKY", "aa_hexdigest": "b94e185e54499b140b2c93947f887f8c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5488.2883, "isoelectric_point": 7.727511024475096 }, "id": "DFOKDJOJIG_553", "locus": "GCA013367855_00529" }, { "type": "cds", "sequence": "contig_1", "start": 297064, "stop": 297417, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTTATAAATTAACTAATTTAATAACAGCAAAAAATGAAACAAAAACCAATTTAATTATTGAAATAAAAAAAGATTTTGAAAATATCGGCAAAGAAATTATTTCAAAATATCAAAAGCTTGTTGAATATATTCAAAAAATTAATCCACTAGCAAATATCGTGGTAACAAATTATGCCAAACCATTTATGGCCTTGCAAGATTTAATTAATTCGATTTACATTTCTTCTTCATATAACAATGAATTTGATTTGGTTGAAATGATACAAGATTTATTAAACAATGTCGCTAAAGAAGTAGCTAAAAAATGCCAATGTTTATATGTAGATATTTATGAAGAAAAATATTGA", "aa": "MPFYKLTNLITAKNETKTNLIIEIKKDFENIGKEIISKYQKLVEYIQKINPLANIVVTNYAKPFMALQDLINSIYISSSYNNEFDLVEMIQDLLNNVAKEVAKKCQCLYVDIYEEKY", "aa_hexdigest": "2fdcb62bf5101cca12f7ae524fa106fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13697.794399999992, "isoelectric_point": 5.486008644104003 }, "id": "DFOKDJOJIG_554", "locus": "GCA013367855_00530" }, { "type": "cds", "sequence": "contig_1", "start": 297514, "stop": 297879, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F5209ED", "UniRef:UniRef90_UPI001F5209ED" ], "nt": "ATGGCTGAAAATCAAAAATATAATTTAAATGACGAAATTAAATATGTTGCTTTAGGAGATTCATTTGCAGCGGGATTTAATAGCAAAGTAGGGTTTAATACTAATGGAAAATTATCAAATGGTAATATAGATGGTTTGGGTTATCCATCATTTTTAGCCTTAAAATTGCGAGATTTAAACTATAGATTAACAAGTTTTTACAATCTAGCAATACCAGCAGGTAATTTAGATATAATTTACGCCTTAACTTCAAATAATGAAAATGATTTAATTGCCAATTCAAAAAGTCTTGATTTGCTTCAATCAATTGATTGGCATGTTTTAAATCCTTCAAAGAATTTTTTTGATGAATTTTTTTTAAATTGA", "aa": "MAENQKYNLNDEIKYVALGDSFAAGFNSKVGFNTNGKLSNGNIDGLGYPSFLALKLRDLNYRLTSFYNLAIPAGNLDIIYALTSNNENDLIANSKSLDLLQSIDWHVLNPSKNFFDEFFLN", "aa_hexdigest": "a770230ee3afeba6063071f75e9accf3", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001F5209ED", "query_cov": 0.9834710743801653, "subject_cov": 1.0, "identity": 0.975, "score": 235.0, "evalue": 2.25e-78, "valid": true, "uniref50_id": "UniRef50_UPI001F5209ED" }, "pscc": { "uniref50_id": "UniRef50_UPI001F5209ED", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F5209ED" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13535.963999999978, "isoelectric_point": 4.7178865432739245 }, "id": "DFOKDJOJIG_555", "locus": "GCA013367855_00531" }, { "type": "cds", "sequence": "contig_1", "start": 297989, "stop": 298204, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAACTAAAAGATGAAGAAACTGGCGCCAAATTAGCTGTAGTTAGTTCAATAAAGTTAGAAGCTTTAAATATAAATGAAGTTATTGTTGCTACTTACCGCCTAATATCTAAGTATTATCCTCAAAAAATTATTGATATAAAATTGCAACCAAGTCAAGATGAAGAAGAAATCTTTAGCCAATTGAATTTATATGCTAAAAATAATAATTGA", "aa": "MPKLKDEETGAKLAVVSSIKLEALNINEVIVATYRLISKYYPQKIIDIKLQPSQDEEEIFSQLNLYAKNNN", "aa_hexdigest": "0d8d68ed0fcf7670b8a5bde62cf052fa", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8125.260899999996, "isoelectric_point": 5.311001396179199 }, "id": "DFOKDJOJIG_556", "locus": "GCA013367855_00532" }, { "type": "cds", "sequence": "contig_1", "start": 298250, "stop": 298699, "strand": "-", "frame": 3, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_A0A077L649", "UniRef:UniRef90_A0A454CAF5" ], "nt": "ATGGCCAAAACCATTAGATTATTAAATGAAAAAATAAGATTATTTGATTTATTTATAATTGTTTTAGATGCACGCTTACCTCTTACTTCGTTTAATAACGATATTTATAAGATGGCTAATAATAAGCCAATTTTATTTATATTAAACAAAAGCGACAAAGCAGATCTATCTAAATTAAAACCTTTTATAAATGAATATGAGAAGAAAGGTGTTGTTGTAACAACTAATTTGAAAGACAAATTAGCCTATAAAAAAATAAATTCAGCTCTTAATAATATTTATTTAAAAGTAAAAGAAAAAAATGCGCAAAAACATAAGTTAACACCAGCATTAAAATGTGTTGTGCTCGGCGTTCCTAATGTTGGTAAAAGTACCTTAATAAATTTATTAGCTGGTTCTAAAGTTGCAAGAGTAGGTGCAATGGCCGGAGTAACAAAGTCAGAGCAATGA", "aa": "MAKTIRLLNEKIRLFDLFIIVLDARLPLTSFNNDIYKMANNKPILFILNKSDKADLSKLKPFINEYEKKGVVVTTNLKDKLAYKKINSALNNIYLKVKEKNAQKHKLTPALKCVVLGVPNVGKSTLINLLAGSKVARVGAMAGVTKSEQ", "aa_hexdigest": "7379ba65a30d002ca09b6ee8a687aae3", "start_type": "ATG", "rbs_motif": null, "genes": [ "ylqF" ], "truncated": "both", "pseudogene": { "start": 297947, "stop": 298723, "observations": { "mutation": [ 297991, 298252, 298714 ], "stop": [ 297991, 298252, 298714 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CAF5", "query_cov": 1.0, "subject_cov": 0.5173611111111112, "identity": 0.987, "score": 276.0, "evalue": 7.12e-92, "gene_start": 1, "gene_end": 149, "reference_start": 9, "reference_end": 157, "reference_sequence": "MFNWFPGHMAKTIRLLNEKIRLFDLFIIILDARLPLTSFNNDIYKMANNKPILFILNKSDKADLSKLKPFINEYEKKGVVVTTNLKDKLAYKKINSALNNIYLKVKEKNAQKHKLTPALKCVVLGVPNVGKSTLINLLAGSKVAKVGAMAGVTKSEQWINCKNYLLLDTPGLLMPKLKDEETGAKLAVVSSIKLEALNINEVIVATYRLISKYYPQKIIDIKLQPSQDEEEIFSQLSLYAKNNNWILKNQLPDIKKAIVQLISYFNNLNNIVYDQCQEKEQNGWKSKI" }, "paralog": false, "identity": 0.9767441860465116, "score": 488.0, "evalue": 1.66e-177, "description": "Internal stop codon at 297991, 298252, 298714. Nonsense mutation around 297991, 298252, 298714.", "psc": { "uniref90_id": "UniRef90_A0A454CAF5", "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_A0A077L649", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CAF5", "UniRef:UniRef50_A0A077L649", "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L649", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L649" ], "product": "Ribosome biogenesis GTPase A" } }, "id": "DFOKDJOJIG_557", "locus": "GCA013367855_00533" }, { "type": "cds", "sequence": "contig_1", "start": 298732, "stop": 298947, "strand": "-", "frame": 1, "gene": "trmH", "product": "TrmH family RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "nt": "ATGTTTGGAACTGAAAGTACTGGAATTCCTAAGAGAATTATGCAAACAAATATTCCTAATTGTTTGCGTATCCCAATGTTTGATGAATGTCGTAGTTTAAATTTAGCCAATAGTGTTGCAATAGTACTATTTGAAGTATTAAGACAAAATGATTTCGCCGGACTATCAAAATTTGAAAGTCAAAAAGGGAAGGATTTTATTTTAAGGAAAGATTGA", "aa": "MFGTESTGIPKRIMQTNIPNCLRIPMFDECRSLNLANSVAIVLFEVLRQNDFAGLSKFESQKGKDFILRKD", "aa_hexdigest": "6bd8369e0e35ce00d282458f1ad0d93b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AH15", "query_cov": 1.0140845070422535, "subject_cov": 0.8674698795180723, "identity": 0.514, "score": 79.0, "evalue": 7.73e-18, "valid": false, "product": "TrmH family RNA methyltransferase", "uniref50_id": "UniRef50_A0A449AH15" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "product": "TrmH family RNA methyltransferase" }, "genes": [ "trmH" ], "id": "DFOKDJOJIG_558", "locus": "GCA013367855_00534" }, { "type": "cds", "sequence": "contig_1", "start": 298951, "stop": 299130, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAGGCCGCTGGACACTTTTTAACTGAAATTCAACATGAAGTTCATGAAAATTATGATGAATTTTTTAAAAAATATGGTGATAAAAATATTTATTATGTAACAAGATACGGTTTAAAAAATTACGTAGATGTTGATTACAAACAAGAATTGCAAACAAATGGTGAACTTTGA", "aa": "MKRKAAGHFLTEIQHEVHENYDEFFKKYGDKNIYYVTRYGLKNYVDVDYKQELQTNGEL", "aa_hexdigest": "274fd3c15d6e6c613318b320804565af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7178.914400000005, "isoelectric_point": 6.257200050354004 }, "id": "DFOKDJOJIG_559", "locus": "GCA013367855_00535" }, { "type": "cds", "sequence": "contig_1", "start": 299526, "stop": 299792, "strand": "-", "frame": 2, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_175972255.1", "SO:0001217", "UniParc:UPI00158B0D19", "UniRef:UniRef100_UPI00158B0D19", "UniRef:UniRef50_P0C0K0", "UniRef:UniRef90_D1J809" ], "nt": "ATGGTATCAAAAGAAAAAAAAGCTGAATTAGTTGTTAAATTCGGACGTAATGCAAAAGACACTGGATATTTACCAGTACAAATAGCAATTTTAACTGAAGATATTGAAAGTTTAAAAGAACATTTCGCAGTAAATAAAAAAGATTTACATTCAATGAGAGGATTCATTGCTAAAGTTAATCACCGTAAAGCATTACTAAATTACTTAAAAGACGAAAACTACAACTTATATTTAGAAACCATCAAAGCTTTAAACATCAGAAAATAA", "aa": "MVSKEKKAELVVKFGRNAKDTGYLPVQIAILTEDIESLKEHFAVNKKDLHSMRGFIAKVNHRKALLNYLKDENYNLYLETIKALNIRK", "aa_hexdigest": "4d92a4440120cc424168adba0d15d867", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158B0D19", "ncbi_nrp_id": "WP_175972255.1", "uniref100_id": "UniRef100_UPI00158B0D19", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B0D19", "RefSeq:WP_175972255.1", "UniRef:UniRef100_UPI00158B0D19" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B0D19", "uniref90_id": "UniRef90_D1J809" }, "psc": { "uniref90_id": "UniRef90_D1J809", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P0C0K0", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P0C0K0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0C0K0" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "DFOKDJOJIG_560", "locus": "GCA013367855_00536" }, { "type": "tRNA", "sequence": "contig_1", "start": 299915, "stop": 299991, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 83.1, "nt": "GCGAGCGTAGCTCAGTTGGTTAGAGCACACGACTGATAATCGTGAGGTCGATGGTTCAAGTCCATTCGTTCGCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 299955, 299957 ], "id": "DFOKDJOJIG_31", "locus": "GCA013367855_00537" }, { "type": "cds", "sequence": "contig_1", "start": 300040, "stop": 300438, "strand": "-", "frame": 1, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02996", "RefSeq:WP_012855500.1", "SO:0001217", "UniParc:UPI0001BC21E4", "UniRef:UniRef100_D1J810", "UniRef:UniRef50_Q98Q72", "UniRef:UniRef90_D1J810" ], "nt": "ATGGCAGATAAAGTAAGATACTATGGTTTAGGTCGTCGTAAATCTTCAGTAGCAAGAGTTTATATTTTACCAGGTAGTGGAAATTTAATTATTAATGGAATAGTTGCAAAAGAATACTTAAATTCAGAAATTTTAATAAAAGATGCTTTAAGTCCTTTTGCAGTAACTGAAACTGCTAACCAATTTGATGTTTTTGCAAATGTTAATGGTGGTGGTTTAACAGGACAAGCTGGAGCAATTAGATTAGGTATTGCTAGAGCATTATTAGAAGCATCTAATAATGAATATAGAAACAAATTAAAGGATGCAGGATTTTTAACACGTGATGCTAGAGTTAAAGAACGTAAGAAATTCGGTTTAAGAAAAGCAAGACGTGCAAGACAATTTTCAAAACGTTAA", "aa": "MADKVRYYGLGRRKSSVARVYILPGSGNLIINGIVAKEYLNSEILIKDALSPFAVTETANQFDVFANVNGGGLTGQAGAIRLGIARALLEASNNEYRNKLKDAGFLTRDARVKERKKFGLRKARRARQFSKR", "aa_hexdigest": "8a2d74830272a058243860cc79a3c920", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC21E4", "ncbi_nrp_id": "WP_012855500.1", "uniref100_id": "UniRef100_D1J810", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21E4", "RefSeq:WP_012855500.1", "UniRef:UniRef100_D1J810" ] }, "ips": { "uniref100_id": "UniRef100_D1J810", "uniref90_id": "UniRef90_D1J810" }, "psc": { "uniref90_id": "UniRef90_D1J810", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q98Q72", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q72" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "DFOKDJOJIG_561", "locus": "GCA013367855_00538" }, { "type": "cds", "sequence": "contig_1", "start": 300441, "stop": 300725, "strand": "-", "frame": 2, "gene": null, "product": "50S ribosomal protein L13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "nt": "ATGGGCGACAATGTGGTTGTGATCAATGCTAAAGATGTACTTTTAACTGCTAAAAAAGATGAAATAAAAGTATATTACCACCACACTGGATACCCAGGTGGATTAAAACAAATTATTGCTGCTGATTTAAGAGCAAAAAAACCAGAATTATTAGTTGAAAAAGCTGTTAGAGGAATGCTTCCACACACCAAATTAGGTCGTAAACAATTTAAAAATTTATATGTTTATGCAGGTTCTGAACACAAACAAGAAGCACAACAACCAGTTAAATTAGAGGTTAAATAA", "aa": "MGDNVVVINAKDVLLTAKKDEIKVYYHHTGYPGGLKQIIAADLRAKKPELLVEKAVRGMLPHTKLGRKQFKNLYVYAGSEHKQEAQQPVKLEVK", "aa_hexdigest": "c123a2eb8dcfc2184a5dcc64e4732d60", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P644", "query_cov": 1.0, "subject_cov": 0.831858407079646, "identity": 0.638, "score": 122.0, "evalue": 4.05e-34, "valid": false, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_A0A3B0P644", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "product": "50S ribosomal protein L13" }, "genes": [], "id": "DFOKDJOJIG_562", "locus": "GCA013367855_00539" }, { "type": "cds", "sequence": "contig_1", "start": 300988, "stop": 301650, "strand": "-", "frame": 1, "gene": "rsmE", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "db_xrefs": [ "COG:COG1385", "COG:J", "EC:2.1.1.193", "RefSeq:WP_020002612.1", "SO:0001217", "UniParc:UPI0003616159", "UniRef:UniRef100_UPI0003616159", "UniRef:UniRef50_A0A448ZY27", "UniRef:UniRef90_D1J812" ], "nt": "ATGTACAAATTTTTTGCTAACAAATTAGAAGATGGAATTTTTTATTTTAGTGATGAAATTCAAAAGCATTTTAAGGTTTTGCGCTTTAATAAAGAAAATATTATTGTAAATTATGAAAATAAGTTTTATGAATGTCAATTTGTTTTTCCTAATAGTGCTAAATTAATTAAAGAGTTAGATATTAATAATGAACTTGACTATAAATTATGTGTTGCAATTCCTTTAATTAAACAAAACCATTTTGAAATAGCATTGCAAAAAGCAGTTGAACTTGGTGCTTATGAAATTTATGTATTTCAAAGTCAATATGTTGATAAATCTAATTTAAATTTTGATTCTAAAAAGCAACGTTATGAAAAAATAATTTTTGAAGCTTGCCAACAAAGTTTTAGAAATTGTATCCCAAGACTTCATGATCCAATTTCATTTTCAGAACTTTGTGTCTTGGATATTAAAAATAAAGTATTAGCTTATGAAAACAAAAAAAATAACGAATTAATTAAAGATTGCCAAGATGCTTTAATTATTGTTGGCCCTGAAGGCGGATTTAGCCAACAAGAAATTGATTATGCTATATCTTGCAATGTTCAAGTTGTTTCACTAACAAAAACGATTTTAAGGGCTGAGACCGCTTTAATTTACATGCTTTCAAAATTAAATTAA", "aa": "MYKFFANKLEDGIFYFSDEIQKHFKVLRFNKENIIVNYENKFYECQFVFPNSAKLIKELDINNELDYKLCVAIPLIKQNHFEIALQKAVELGAYEIYVFQSQYVDKSNLNFDSKKQRYEKIIFEACQQSFRNCIPRLHDPISFSELCVLDIKNKVLAYENKKNNELIKDCQDALIIVGPEGGFSQQEIDYAISCNVQVVSLTKTILRAETALIYMLSKLN", "aa_hexdigest": "19cc3f0967adf6c2fed76acd18cc7dc3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003616159", "ncbi_nrp_id": "WP_020002612.1", "uniref100_id": "UniRef100_UPI0003616159", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003616159", "RefSeq:WP_020002612.1", "UniRef:UniRef100_UPI0003616159" ] }, "ips": { "uniref100_id": "UniRef100_UPI0003616159", "uniref90_id": "UniRef90_D1J812" }, "psc": { "uniref90_id": "UniRef90_D1J812", "gene": "RsmE", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "ec_ids": [ "2.1.1.193" ], "uniref50_id": "UniRef50_A0A448ZY27", "cog_id": "COG1385", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZY27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZY27" ], "product": "Ribosomal RNA small subunit methyltransferase E" }, "genes": [ "rsmE" ], "id": "DFOKDJOJIG_563", "locus": "GCA013367855_00540" }, { "type": "cds", "sequence": "contig_1", "start": 301842, "stop": 302102, "strand": "-", "frame": 2, "gene": null, "product": "leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003A3F0D4", "UniRef:UniRef90_UPI0003A3F0D4" ], "nt": "TTGGATAAAGAAACCACAAAAGTTACTATTCCAAACAACATAAAAGAAATTGATGATAGTGCATTTCTTGGCTGTCAAAACCTTAAAAAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGATGAGGCATTTTACAATACAAACATTGAATCTATTACTATTCCTGCTTCAGTAAAAAAATTTGGCTATAGTGCGTTTTTTGGCTGCAAAAACCTTAAAGAAGTAATTTATAAAGGTGATGCAAGTAATATAGGTTGA", "aa": "MDKETTKVTIPNNIKEIDDSAFLGCQNLKKVILNEGLEKIGDEAFYNTNIESITIPASVKKFGYSAFFGCKNLKEVIYKGDASNIG", "aa_hexdigest": "c2e6c3e9f44e3b807cc0d4356cb977f1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0003A3F0D4", "query_cov": 1.0, "subject_cov": 0.8269230769230769, "identity": 1.0, "score": 118.0, "evalue": 7.5e-33, "valid": true, "product": "leucine-rich repeat domain-containing protein", "uniref50_id": "UniRef50_UPI0003A3F0D4" }, "pscc": { "uniref50_id": "UniRef50_UPI0003A3F0D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003A3F0D4" ], "product": "leucine-rich repeat domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_564", "locus": "GCA013367855_00541" }, { "type": "cds", "sequence": "contig_1", "start": 302329, "stop": 302487, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_241862907.1", "SO:0001217", "UniParc:UPI001F525782", "UniRef:UniRef100_UPI001F525782", "UniRef:UniRef50_UPI001C37BB16", "UniRef:UniRef90_UPI001F525782" ], "nt": "ATGTTTATAGTTTTAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTTTACAACTAGAAAACGCACTTTTGCCAACTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTCTGTATTATTAAATGCATAA", "aa": "MFIVLNASAPIFSRPSFKITFLRFLQLENALLPTSFTEPGIVIDSMSVLLNA", "aa_hexdigest": "4e6c8a234f3353ac29906b55ee562b48", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001F525782", "ncbi_nrp_id": "WP_241862907.1", "uniref100_id": "UniRef100_UPI001F525782", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F525782", "RefSeq:WP_241862907.1", "UniRef:UniRef100_UPI001F525782" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F525782", "uniref90_id": "UniRef90_UPI001F525782" }, "psc": { "uniref90_id": "UniRef90_UPI001F525782", "uniref50_id": "UniRef50_UPI001C37BB16" }, "pscc": { "uniref50_id": "UniRef50_UPI001C37BB16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001C37BB16" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5771.787199999999, "isoelectric_point": 5.937878608703613 }, "id": "DFOKDJOJIG_565", "locus": "GCA013367855_00542" }, { "type": "cds", "sequence": "contig_1", "start": 303947, "stop": 305659, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05725.18", "PFAM:PF12799.13", "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "TTGGATAAAAAAACCACAAAAGTTACTATTCCAAACAATGTAAAAGAAATCGGCGAAGGTGCGTTTTCTGGTTGTAGAAACATTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGACTCTGGTGCATTTCAAGATACAAATATTGAATCTATTACTATTCCTGATTCAGTAGAAGAAATTGGCAAATATGCGTTTTCTGATTGCAAAATCCTTAAAGAAGTAACTTTAAATGAAGGACTTGAAAAAATTGGTGCTGAGGCATTTAAATATACAAACATTAAATCTATTACTATTCCTGGTTCGGTAAAAGAAATTGGCGATTTTGCATTTAATTCTTCAATAATTTCATATATTTCAATTAATTCAAATAATAAAAATTTTGAAATTAAAGATAACTTTTTTATTGATAAAAATAATAAAAAGATATTAGCATATTTGGATAAAAAAACCACAAAAGTTACTATTCCAAACAATGTAAAAGAAATCGGCGAAGGTGCGTTTTCTGGTTGTAGAAACATTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGACTCTGGTGCATTTCAAGATACAAATATTGAATCTATTACTATTCCTGATTCAGTAGAAGAAATTGGCAAATATGCGTTTTATAATTGCACAAACCTTAAAGAAGTAACTTTAAATGAAGGACTTGAAAAAATTGGCACTGGTGCATTTGGGAGGACAAACATTGAATCTATTACTATTCCTGATTCAATAAAAGAAATTGACGATTGGGCATTTGATTTTTCAATAATTTCACATATTTCAATTAATTCAAATAATAAAAATTTTGAAATTAAAGATAACTTTTTTATTGATAAAAATAATGAAAAAATATTAGCATATTTGGATAAAAAAATTACAAAAGTTACTATTCCTGGTTCAGTAAAAGAAATTGGCAAAAGAGCATTTTCTAATTGCAAATACCTTAAAGAAGTAATTTTAAATGAAGAACTTGAAAAAATTGGCGCTGAGGCATTTCGAGATACAAACATTAAATCTATTACTATTCCTAATTCAGTAAAAGAAATTGGAGATAACGCGTTTTCTGGTTGCTCAAACCTTAAAGAAGTAACTTTAAATGAAGAACTTGAAAAAATTGGCGCTGGTGCATTTGGGGGGACAAACATTGAATCTGTTACTATTCCTGGTTCAATAAAAGAAATTGACAAAAGTACATTTTCTGGTTGCAAATGCCTTAAAGAAGTAATTTTAAATGAAGGTCTTAAAAAAATCGGCGATTGGGCATTTCAATATACAAAAATTGAATCTATTACTATCCCTGGTTCAGTAAAAGAAATTTGCAAATATGCGTTTTATAATTGCACAAACCTTAAAGAAGTATTTTTAAATGAAGGACTTGAAAAAATTGGCGAAAGTGCGTTTTCTGGTTGCAAATACCTTAAAGAAGTAACTTTAAATGAAGGACTTGAAAAAATTGGTGCCGGTGCATTTGAGAGGACAAACATTGAATCTGTTACTATTCCTGGTTTAGTAAAAGAAATTGACAAAAGTACATTTTCTGGTTGCAAATGCCTTAAAGAAGTAACTTTAAATGAAGGACTTGAAAAAATCGGTGCTGAAGCATTTCGAGATACAAACATTAAATCTATTACTATTCCTGGTTTAGTAAAAGAAATCGGCGAAAGAGCGTTTTATGGTTGCATATACCTTTGA", "aa": "MDKKTTKVTIPNNVKEIGEGAFSGCRNIKEVILNEGLEKIDSGAFQDTNIESITIPDSVEEIGKYAFSDCKILKEVTLNEGLEKIGAEAFKYTNIKSITIPGSVKEIGDFAFNSSIISYISINSNNKNFEIKDNFFIDKNNKKILAYLDKKTTKVTIPNNVKEIGEGAFSGCRNIKEVILNEGLEKIDSGAFQDTNIESITIPDSVEEIGKYAFYNCTNLKEVTLNEGLEKIGTGAFGRTNIESITIPDSIKEIDDWAFDFSIISHISINSNNKNFEIKDNFFIDKNNEKILAYLDKKITKVTIPGSVKEIGKRAFSNCKYLKEVILNEELEKIGAEAFRDTNIKSITIPNSVKEIGDNAFSGCSNLKEVTLNEELEKIGAGAFGGTNIESVTIPGSIKEIDKSTFSGCKCLKEVILNEGLKKIGDWAFQYTKIESITIPGSVKEICKYAFYNCTNLKEVFLNEGLEKIGESAFSGCKYLKEVTLNEGLEKIGAGAFERTNIESVTIPGLVKEIDKSTFSGCKCLKEVTLNEGLEKIGAEAFRDTNIKSITIPGLVKEIGERAFYGCIYL", "aa_hexdigest": "f39412449b3bf030128df37bc0a3410b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05725.18", "name": "FNIP", "length": 15, "aa_cov": 0.02631578947368421, "hmm_cov": 1.0, "evalue": 2.7731838473212397e-08, "score": 29.48890495300293, "start": 150, "stop": 164 }, { "id": "PF12799.13", "name": "LRR_4", "length": 33, "aa_cov": 0.056140350877192984, "hmm_cov": 0.9393939393939394, "evalue": 5.604231780350681e-09, "score": 31.721372604370117, "start": 455, "stop": 486 }, { "id": "PF13306.12", "name": "LRR_5", "length": 114, "aa_cov": 0.2, "hmm_cov": 1.0, "evalue": 1.3550967915869832e-172, "score": 560.5006103515625, "start": 148, "stop": 261 }, { "id": "PF13855.12", "name": "LRR_8", "length": 37, "aa_cov": 0.06315789473684211, "hmm_cov": 1.0, "evalue": 1.4938562234515431e-22, "score": 74.82691955566406, "start": 351, "stop": 386 } ], "seq_stats": { "molecular_weight": 62974.071700000124, "isoelectric_point": 5.110984992980956 }, "id": "DFOKDJOJIG_566", "locus": "GCA013367855_00543" }, { "type": "cds", "sequence": "contig_1", "start": 306042, "stop": 306269, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTGTGATTTTTCAAGTATTTGATTCAGTTAGTTCGAATTTAAATTTTTTATTTAAATTTGATGAATCTTCTTCTTTTGTAGGATTTTCTGGTTTAGGTTTTGGTTTATTTTCATCAGGCTTTGAATCTTCTTTGGTAGGATTTTCTGGTTTAGGTTCAGGTTTATTTTCATCAGGTTTTGAATCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTTTATGA", "aa": "MNFVIFQVFDSVSSNLNFLFKFDESSSFVGFSGLGFGLFSSGFESSLVGFSGLGSGLFSSGFESSLFSFEFCFTL", "aa_hexdigest": "806a6c27e76b3fb900975a34fb60cd9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8112.961999999992, "isoelectric_point": 4.0500284194946286 }, "id": "DFOKDJOJIG_567", "locus": "GCA013367855_00544" }, { "type": "cds", "sequence": "contig_1", "start": 307020, "stop": 307523, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "ATGATAGAAAAAAAGCAAGACCTTTATGATCTTGCTTTTTTTCCTTATTTGGAATTAACCCCACATTTTTCTACGCTTCCTAAAAATAATAAAAAAATATTAGCATATTTGGATAAAAAAACCACAAAAGTTGTTATTCCAAACAATGTAAAAAAAATTGGTGAAAGTGCGTTTTCTAATTGCGAAAACCTTAAAGAAGTAAGTTTAAATGAAGGTCTTGAAAAAATTGGTGCAGAGACATTTTATGATACAAAAATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGCGAAAGAGCGTTTTGTAATTGCGAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTAAAAAAATCGGCGCTAGTGCATTTCCAAATACAAAAATTGAATCTATTACTATTCCAAGCTCGGTAAAAGAAATTGGTGAAAGTGCGTTTTCTAATTGCAAAAACCTTAAAGAAGTAATTTATAAAGGTGATGCAAGTAATATAAATTGA", "aa": "MIEKKQDLYDLAFFPYLELTPHFSTLPKNNKKILAYLDKKTTKVVIPNNVKKIGESAFSNCENLKEVSLNEGLEKIGAETFYDTKIESITIPGSVKEIGERAFCNCENLKEVILNEGLKKIGASAFPNTKIESITIPSSVKEIGESAFSNCKNLKEVIYKGDASNIN", "aa_hexdigest": "e1c7f54757112684402008d55b5275f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 119, "aa_cov": 0.7125748502994012, "hmm_cov": 1.0, "evalue": 9.196872545120179e-42, "score": 137.6149444580078, "start": 37, "stop": 155 } ], "seq_stats": { "molecular_weight": 18583.15490000001, "isoelectric_point": 6.619548606872559 }, "id": "DFOKDJOJIG_568", "locus": "GCA013367855_00545" }, { "type": "cds", "sequence": "contig_1", "start": 307652, "stop": 308032, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGCTTATAGTGAATTTATGGTAATGCAGCAAGAGGCTAAATTAAACAACGATTATAGCCTTGTGGATGCATATGAAAGAGATTTAAATTGGAGGAGAAATTATAGTAAAAAAATATATGACAAATTAAGCGACATAGCTATAAGCAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATGGTTATTAAATCCTTTAAAAATAAAAAAATCAAAATCATTGATCTAATAAATTTAGGGTTTAAAAAAACTTCTATAAAATCAGCTATAAAAAGACTTATTGAAATAAAAGTTTTAGATGGTGAGTTTTATAAAAATATAAACTTCTAA", "aa": "MGAYSEFMVMQQEAKLNNDYSLVDAYERDLNWRRNYSKKIYDKLSDIAISKILKSKIVISESYYQKLKTNKVLLEVFMVIKSFKNKKIKIIDLINLGFKKTSIKSAIKRLIEIKVLDGEFYKNINF", "aa_hexdigest": "ee37223c2ae339e2ac653b87f5308663", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14903.450799999982, "isoelectric_point": 9.719978904724119 }, "id": "DFOKDJOJIG_569", "locus": "GCA013367855_00546" }, { "type": "cds", "sequence": "contig_1", "start": 308271, "stop": 308435, "strand": "+", "frame": 3, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "RefSeq:WP_175972262.1", "SO:0001217", "UniParc:UPI00158EAE53", "UniRef:UniRef100_UPI00158C7902", "UniRef:UniRef50_A0A9Q9F2D4", "UniRef:UniRef90_A0A454C8Z1" ], "nt": "ATGAACAAAGCCATAGCCAATTATTTTGGAGTTGAATATTTGCAAATGTTTGGTATATTGCGTAGTCAAGAAAAAAAAGTTACAACATTAAAATTAGGAAATTTAAACATCAAATGTAATGTTTATAAATTTTCTACACAACGTTATTTGTTAATGCAAATTTAA", "aa": "MNKAIANYFGVEYLQMFGILRSQEKKVTTLKLGNLNIKCNVYKFSTQRYLLMQI", "aa_hexdigest": "cf685e3d1d55bbc71ebe10a3c2a6ac0f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158EAE53", "ncbi_nrp_id": "WP_175972262.1", "uniref100_id": "UniRef100_UPI00158C7902", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EAE53", "RefSeq:WP_175972262.1", "UniRef:UniRef100_UPI00158C7902" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C7902", "product": "MAGa4850 family ICE element protein", "uniref90_id": "UniRef90_A0A454C8Z1" }, "psc": { "uniref90_id": "UniRef90_A0A454C8Z1", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_A0A9Q9F2D4" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4" ] }, "genes": [], "id": "DFOKDJOJIG_570", "locus": "GCA013367855_00547" }, { "type": "cds", "sequence": "contig_1", "start": 308845, "stop": 308952, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E466C14", "UniRef:UniRef90_UPI001E466C14" ], "nt": "GTGGTAGATTACATTACAAGACCAGATAAATGCGTTGTATTTAAAGAAGATGAAAATAATTATGATGCTATGAAACTACTTCTTACTAAGGAAGATAAAATAAATTGA", "aa": "MVDYITRPDKCVVFKEDENNYDAMKLLLTKEDKIN", "aa_hexdigest": "4319b445e246145b5e30e86e9d399845", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 308845, "stop": 309013, "observations": { "insertion": [ 308995 ], "mutation": [ 308950 ], "stop": [ 308950 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E466C14", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 1.0, "score": 73.9, "evalue": 7.88e-17, "gene_start": 1, "gene_end": 35, "reference_start": 1, "reference_end": 35, "reference_sequence": "MVDYITRPDKCVVFKEDENNYDAMKLLLTKEDKINWYRQHLKINQNSKQSVFITY" }, "paralog": false, "identity": 0.9636363636363636, "score": 105.0, "evalue": 8.21e-32, "description": "Internal stop codon at 308950. Frameshift due to insertion around 308995. Nonsense mutation around 308950.", "psc": { "uniref90_id": "UniRef90_UPI001E466C14", "uniref50_id": "UniRef50_UPI001E466C14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E466C14", "UniRef:UniRef50_UPI001E466C14" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E466C14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E466C14" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4192.786300000001, "isoelectric_point": 4.716124534606934 }, "id": "DFOKDJOJIG_571", "locus": "GCA013367855_00548" }, { "type": "cds", "sequence": "contig_1", "start": 309510, "stop": 309641, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9H9", "UniRef:UniRef90_A0A454C9H9" ], "nt": "ATGGGTGGTAATAAGGAATTTATGTTAATTCGGCAAGAGGCTAAATTAAACAAAAATTATAGCCTTGTGGATGCATTTGAAATAAATTTAAATTGGAAGAAAAAATATAATAAAAAATCTATGACAAATTAA", "aa": "MGGNKEFMLIRQEAKLNKNYSLVDAFEINLNWKKKYNKKSMTN", "aa_hexdigest": "6e99f244a6c118649ec36df627fee4ee", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 309400, "stop": 309641, "observations": { "deletion": [ 309487 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9H9", "query_cov": 1.0, "subject_cov": 0.5584415584415584, "identity": 0.8370000000000001, "score": 75.9, "evalue": 3.38e-17, "gene_start": 1, "gene_end": 43, "reference_start": 35, "reference_end": 77, "reference_sequence": "MGLITNKKKSKIIKVLLFSIIHKKISLKIFEKNFMGGNKEFMLMKQEAKSNNDYSLVDAFKINLNWKKNYNKKSMTN" }, "paralog": false, "identity": 0.825, "score": 125.0, "evalue": 2.06e-39, "description": "Frameshift due to deletion around 309487.", "psc": { "uniref90_id": "UniRef90_A0A454C9H9", "uniref50_id": "UniRef50_A0A454C9H9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9H9", "UniRef:UniRef50_A0A454C9H9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9H9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9H9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5138.941600000002, "isoelectric_point": 9.822999382019042 }, "id": "DFOKDJOJIG_572", "locus": "GCA013367855_00549" }, { "type": "cds", "sequence": "contig_1", "start": 310039, "stop": 310620, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "ATGGATAAAAATAATAAAAAGATATTAGCATATTTGGATAAGGAGGCCACAAAAGTTACTATTCCTGATTCAGTAAAAGAAATTTGCAAAGGTGCGTTTTATGGTTGCAAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGTGCCGAGGCATTTAAATATACAAACATTGAATCTATTACTATTCCTGGCTCAGTAAAAGAAATTGGCGTTGAGGCATTTCGCCATACAAACATTGAATCTATTACTATTCCTGGCTCAGTAAAAGAAATTGGCGAAAGAGCGTTTTTTGGTTGCCAAAACCTTAATGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGAGGCATTTTATCATACAAGCATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGACGAAAGTACATTGTCTGGTTGCGAAAACCTTGGAGAAGTAGTTTTAAATGAAGGCCTTGAAAGAATTGGCGATTATGCATTTAGGTATGCAAAAATTTTATCTATTACTATTCCTGGCTCAGTAAAAGAAATTGGCGTTGATGCATTTCTTGGTTGCTGA", "aa": "MDKNNKKILAYLDKEATKVTIPDSVKEICKGAFYGCKNLKEVILNEGLEKIGAEAFKYTNIESITIPGSVKEIGVEAFRHTNIESITIPGSVKEIGERAFFGCQNLNEVILNEGLEKIGAEAFYHTSIESITIPGSVKEIDESTLSGCENLGEVVLNEGLERIGDYAFRYAKILSITIPGSVKEIGVDAFLGC", "aa_hexdigest": "fb7e24e6d3d320173124e328696c1cf5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 123, "aa_cov": 0.6373056994818653, "hmm_cov": 1.0, "evalue": 4.168946730800101e-58, "score": 190.4422149658203, "start": 71, "stop": 193 } ], "seq_stats": { "molecular_weight": 21039.837000000003, "isoelectric_point": 4.892780113220215 }, "id": "DFOKDJOJIG_573", "locus": "GCA013367855_00550" }, { "type": "cds", "sequence": "contig_1", "start": 310684, "stop": 310893, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "TTGGATAAGGAGGCCACAAAAGTTACTATTCCTGATTCAGTAAAAGAAATTGGCAAAGGTGCGTTTTCTAATTGCGAAAACCTTGAAGAAGTAAGTTTAAATGAAGGTCTTGAAAAAATTGGTGATGAGGCATTTTCAAGTACAAAAATTGAATCTATTACTATCCCTGGTTCAATAAAAGAAATTGGCGAAGGTGCATTATCTTTTTGA", "aa": "MDKEATKVTIPDSVKEIGKGAFSNCENLEEVSLNEGLEKIGDEAFSSTKIESITIPGSIKEIGEGALSF", "aa_hexdigest": "53383e4b061195f307849ba2b65df984", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 65, "aa_cov": 0.9420289855072463, "hmm_cov": 1.0, "evalue": 4.313019181183494e-21, "score": 70.79985809326172, "start": 4, "stop": 68 } ], "seq_stats": { "molecular_weight": 7333.124999999996, "isoelectric_point": 4.3830480575561515 }, "id": "DFOKDJOJIG_574", "locus": "GCA013367855_00551" }, { "type": "cds", "sequence": "contig_1", "start": 311227, "stop": 311568, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAATCAAAAAAAATTTTGTTGACCATGGGGGGGATACTTCCCTTGGTTATTTCAGCGCCATTAGTTGCTGCTGGATGCAAGGAGCCAAACAAACCAGAAGTTAAACCAGAGGATCCAAAGAAACCAGAAGGTGAAAAACCTGGCACAACTCCTGAAGTTAAACCAGAGGATCCAAAGAAACCAGAAGGTGAAAAACCAGGAACAACACCTGAAGTTAAACCAGAGCCAAAACCCGAACCAAAACCCGAACCAAAACCAGAAAATCCTACCAAAGAAGAAGATTTATTAAATTTTAGGAAAAATTTTAAATTTGAACTAACTAAATCAAATACTTGA", "aa": "MKKSKKILLTMGGILPLVISAPLVAAGCKEPNKPEVKPEDPKKPEGEKPGTTPEVKPEDPKKPEGEKPGTTPEVKPEPKPEPKPEPKPENPTKEEDLLNFRKNFKFELTKSNT", "aa_hexdigest": "1e5479abb448a6389880175d18d2997b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12471.339999999982, "isoelectric_point": 8.887692070007326 }, "id": "DFOKDJOJIG_575", "locus": "GCA013367855_00552" }, { "type": "cds", "sequence": "contig_1", "start": 311857, "stop": 313002, "strand": "-", "frame": 1, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "COG:COG0206", "COG:D", "GO:0000910", "GO:0003924", "GO:0005525", "RefSeq:WP_020002414.1", "SO:0001217", "UniParc:UPI00036AB532", "UniRef:UniRef100_A0A2K9YSW7", "UniRef:UniRef50_A0A3L9ZYL3", "UniRef:UniRef90_D1J814" ], "nt": "ATGGCAAAAGAATTAAATAATTATAACCCGGTGGCAAAAATAATTGTTATTGGAGTAGGTGGTGGCGGAAATAATAGTGTTGAAACTATGATAAATAGCCATTTAGATAGTTTTCAAATTATTGCTGCCAATACCGATAAACAAGTATTAGCAAAATTTCCTCAAGAATGCGTTTTACATTTAGGTGATGAACGTGGAATTGGCGCGGGTGCTAACCCTGAAATTGGCAAAACTGCTGCTGAATCTTCGCGTGAAGAAATCAAAAGTAGATTGCAAGGAGCTGATCTAGTAATTATAACTGCAGGAATGGGTGGTGGAACCGGCACAGGTGCAGCGCCCGTGATTGCACAAATAGCCAAGGAATGCAATGCTTTAGTAGTTGCAGTTGTAACTACTCCGTTTGACTTTGAAGGTCCAAAGAGAATGAGAATTGCAAAGCAAGGCTTACAAGAAATTAAAAAATGTGTTGATTCATATATTGTTATTTCAAACAATAAACTATTACAACAATATGGAAATATTTCTTTTTCAGATGCATTTATTTGCGCAAATAATGTCTTAAAGCAAACTATTAGAACAATAGTTGATGTTATTGCCACCCCAAGCATTATTAATCTTGATTTTGCAGATTTATCAACCATTATTAAAAATAAAGGTGAAACAGTAATCGGCATTGGCCAAGCAAATGGCCAAGATCGGGCTGTAAAAGCAATCACATCTGCAATAACAAGCCCAATTTTAGAATCAAGTGTTGTTGGGGCTAGCGATGCAATAGTAAATTTCAGTGCTTCTCAAAAAGTAACTTTAAATGAAATTCAAAGTGCTTTGGGAGCTATGAGGGAAATTGTTGGTAATGAAATTAACATTATTTTTGGTATAACAACTCTAGAATCTGAAGAAAGCAACAAATTAGGTGAGCTATTTGTCTCGGTAATCGCCACGGGATTAAGAAAAGACGCTCCCAAAGACATTGATCAAATTCAAGACGAAGTAATCAATGTAATAAAAAAAGACGATTTAAATTATGTTAACGACGAAACAAAGGAATTCTTTGTATCTGAAGGAACATTTAAAACACAATCATTTTTTTCAATGGATAATGATGAAGATTCAAACGATGAACTTGCAGATATATTAAAACACTAA", "aa": "MAKELNNYNPVAKIIVIGVGGGGNNSVETMINSHLDSFQIIAANTDKQVLAKFPQECVLHLGDERGIGAGANPEIGKTAAESSREEIKSRLQGADLVIITAGMGGGTGTGAAPVIAQIAKECNALVVAVVTTPFDFEGPKRMRIAKQGLQEIKKCVDSYIVISNNKLLQQYGNISFSDAFICANNVLKQTIRTIVDVIATPSIINLDFADLSTIIKNKGETVIGIGQANGQDRAVKAITSAITSPILESSVVGASDAIVNFSASQKVTLNEIQSALGAMREIVGNEINIIFGITTLESEESNKLGELFVSVIATGLRKDAPKDIDQIQDEVINVIKKDDLNYVNDETKEFFVSEGTFKTQSFFSMDNDEDSNDELADILKH", "aa_hexdigest": "0f5c85322a9a468cf81cbe118673640a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00036AB532", "ncbi_nrp_id": "WP_020002414.1", "uniref100_id": "UniRef100_A0A2K9YSW7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036AB532", "RefSeq:WP_020002414.1", "UniRef:UniRef100_A0A2K9YSW7" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YSW7", "uniref90_id": "UniRef90_D1J814" }, "psc": { "uniref90_id": "UniRef90_D1J814", "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_A0A3L9ZYL3", "cog_id": "COG0206", "cog_category": "D", "go_ids": [ "GO:0000910", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3L9ZYL3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3L9ZYL3" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "DFOKDJOJIG_576", "locus": "GCA013367855_00553" }, { "type": "cds", "sequence": "contig_1", "start": 313010, "stop": 313150, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCAATAGATAATATTTTAGAATGTCAAATCAAACAAAATGCTTTAAATAATGAAATTAGTAGGGAATTATTTACAATTGAAGTTTATAGTAATTCCAAAAAGTATTACAATTTAATGCTAAACGATATAAATTAG", "aa": "MLAIDNILECQIKQNALNNEISRELFTIEVYSNSKKYYNLMLNDIN", "aa_hexdigest": "18d5c39f4a1dba767c77377ba2b8f4bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5438.170000000001, "isoelectric_point": 4.655875205993653 }, "id": "DFOKDJOJIG_577", "locus": "GCA013367855_00554" }, { "type": "cds", "sequence": "contig_1", "start": 313409, "stop": 313744, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTAAACTAGTTAAAAAAACTAATATTAAGCAAATTTATTCTTTAAAAACAATATCAAATGCTAATAGTCTAAGATTGCAAGAAATTTTTAGTAGCAACGATTTAAAAATTGCCAAAATATTTTGCTTAGATAGTTTAAAGACAAATTCAGTTTTGTTTAAAAATTCAAAACAAAAAGTAGCTATTTTTGTTGATGTATTAGATGATAAAGCTAAATTTATTTTAAACATTAATAAATCAAATATAGTAAGCCAAGTATTAGATTTAGGACTAAACAAATTAAAAGCAAGAATGTTACAATGCAATTTATTAAATACAGAAAGCAATTGA", "aa": "MEFKLVKKTNIKQIYSLKTISNANSLRLQEIFSSNDLKIAKIFCLDSLKTNSVLFKNSKQKVAIFVDVLDDKAKFILNINKSNIVSQVLDLGLNKLKARMLQCNLLNTESN", "aa_hexdigest": "ee1146e1561ab65057c5c754e15bccb3", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12621.762399999981, "isoelectric_point": 9.76794338226318 }, "id": "DFOKDJOJIG_578", "locus": "GCA013367855_00555" }, { "type": "cds", "sequence": "contig_1", "start": 313844, "stop": 314077, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E400CB7", "UniRef:UniRef90_UPI001E400CB7" ], "nt": "ATGAAAAAAATTATTATTGCTGCATATAAATTTTTAAAAAATAGTATCGAAGTAGAAGTTATGGAAAAAACTAATACTTTATCTTTTTGCATTTATAAAAAAGCAATTAATAATATTTATTTAACTGAAAGCGATATAAAAAATTTTATAAAGCAAACAAAGAATGAAATTCAAAAATTAATATCAGCAAGCATAAATGAAGTTATTGTTATGTTGTCTGAAGATAGCAAATGA", "aa": "MKKIIIAAYKFLKNSIEVEVMEKTNTLSFCIYKKAINNIYLTESDIKNFIKQTKNEIQKLISASINEVIVMLSEDSK", "aa_hexdigest": "29c293c4bbf54aa26858a8c72d03686c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 313756, "stop": 314077, "observations": { "insertion": [ 313786 ], "mutation": [ 313846 ], "stop": [ 313846 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E400CB7", "query_cov": 1.0, "subject_cov": 0.7264150943396226, "identity": 1.0, "score": 147.0, "evalue": 2.03e-44, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MKKIIIAAYKFLKNSIEVEVMEKTNTLSFCIYKKAINNIYLTESDIKNFIKQTKNEIQKLISASINEVIVMLSEDSKWNINSKLLTLNNDNFNISNLKILDQTSII" }, "paralog": false, "identity": 0.9905660377358491, "score": 194.0, "evalue": 1.92e-65, "description": "Internal stop codon at 313846. Frameshift due to insertion around 313786. Nonsense mutation around 313846.", "psc": { "uniref90_id": "UniRef90_UPI001E400CB7", "uniref50_id": "UniRef50_UPI001E400CB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E400CB7", "UniRef:UniRef50_UPI001E400CB7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E400CB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E400CB7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8904.433299999999, "isoelectric_point": 9.04112682342529 }, "id": "DFOKDJOJIG_579", "locus": "GCA013367855_00556" }, { "type": "cds", "sequence": "contig_1", "start": 314178, "stop": 314984, "strand": "-", "frame": 2, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0016434", "RefSeq:WP_114091198.1", "SO:0001217", "UniParc:UPI000DED655C", "UniRef:UniRef100_D1J816", "UniRef:UniRef50_Q6KHR2", "UniRef:UniRef90_D1J816" ], "nt": "ATGGAACATATTCCAGTTCTATTAAATGAAGTAATAGAACAATTATCAATAAAACCAGACGGAATATATGTTGATTTAACATTAGGCCGTGGTGGTCATAGCCAAGAAATTTTAAAAAGACTAACAACTGGAAAATTAATTTGTTTTGATAAAGATATTCAAGCTATAAACGAAAGTAGAGAAAAATTAATAAAAATAAGCCAAAACTTTATTTTAATAAAAAGTGATTTTAGAAACATTACTTGTGAACTAAAAAAACTAAATATTGATAAAGTCGATGGAATATTAGCAGATTTAGGAGTTTCTAGTCCTCAATTAGATGATCCAAATAGAGGATTTTCTTATTCAATCAATTCTGATTTAGATATGAGAATGGATTTAGATAATACTCTAACTGCAAAAGAAATTATTAATAATGCAACTGAAAAAGAAATTATAGATATATTGCAATCTAATGCTGATGTAAAATTAAGTAATTTAATTGCGAAAGCTATTGTAAAAAATCGACCAATATCTGATTCATTTACGTTAAATCAAATAATAAAGGATTCATTGCCAGCTAAAATAGTAAGACAAAAAAATCCTTCTAAAGCAGTTTTTCAAGCTTTTAGAATAGCAGTGAATGATGAGCTAAATGCCTTAAGTGAAATGCTAAGCCAACTTCCTAATTTATTAAAAAAAGACGGAAAAATAGCGATTATAACATTTCATAGTAAAGAAGACGTTATAGTTAAAAAGTTTTATCAAAATTTAAATTATTTTGACCCTAAGTTAAAAAAAATACTAATTGTTGAAACTCATAAGTGA", "aa": "MEHIPVLLNEVIEQLSIKPDGIYVDLTLGRGGHSQEILKRLTTGKLICFDKDIQAINESREKLIKISQNFILIKSDFRNITCELKKLNIDKVDGILADLGVSSPQLDDPNRGFSYSINSDLDMRMDLDNTLTAKEIINNATEKEIIDILQSNADVKLSNLIAKAIVKNRPISDSFTLNQIIKDSLPAKIVRQKNPSKAVFQAFRIAVNDELNALSEMLSQLPNLLKKDGKIAIITFHSKEDVIVKKFYQNLNYFDPKLKKILIVETHK", "aa_hexdigest": "0184253a5e0f8c695f0b4111da5dcd67", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "ups": { "uniparc_id": "UPI000DED655C", "ncbi_nrp_id": "WP_114091198.1", "uniref100_id": "UniRef100_D1J816", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DED655C", "RefSeq:WP_114091198.1", "UniRef:UniRef100_D1J816" ] }, "ips": { "uniref100_id": "UniRef100_D1J816", "uniref90_id": "UniRef90_D1J816" }, "psc": { "uniref90_id": "UniRef90_D1J816", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_Q6KHR2", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0016434" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR2" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "DFOKDJOJIG_580", "locus": "GCA013367855_00557" }, { "type": "cds", "sequence": "contig_1", "start": 314985, "stop": 315425, "strand": "-", "frame": 2, "gene": "mraZ", "product": "Transcriptional regulator MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "RefSeq:WP_012855507.1", "SO:0001217", "UniParc:UPI0001BC21EB", "UniRef:UniRef100_D1J817", "UniRef:UniRef50_Q6KHR3", "UniRef:UniRef90_D1J817" ], "nt": "ATGTTTGGTAAATATTATCGCCAAATTGATGATAAAAATAGAGTTGTTGTTCCGCCTAAATTACTACAAGAATTAGGCAAGGAATTCTATATCACAATTGGAATAGATAAAAACTTAGTATTAAGAACGGTTTCTGAGTTTCAAAAAATGAAAACTAAATTGGAAGAAGGAAACACACTTAACAAAAACGTAAGAGATTTTATTCGCTACATTTTTGGAAATACTGAAGTTGTATCTCCTGATAAAAATAACAGAATCATGATTCCCAAACATCTTTTAAATAAAGCCGCTATCGACAAAGAAGTTGTTTTCCTAGGAACTGGTGATACTTGTGAATTATTTGCTAAAGAAGTTTATGATGAACAAGAAAGCTACTATGAAAATGATGATAATGTAAATGACTTGGCTCAAAAATTATTTGAAGCAGGAGTTAAGTTCTAA", "aa": "MFGKYYRQIDDKNRVVVPPKLLQELGKEFYITIGIDKNLVLRTVSEFQKMKTKLEEGNTLNKNVRDFIRYIFGNTEVVSPDKNNRIMIPKHLLNKAAIDKEVVFLGTGDTCELFAKEVYDEQESYYENDDNVNDLAQKLFEAGVKF", "aa_hexdigest": "90a8ff3ff46d05845bdb9147a418c90f", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001BC21EB", "ncbi_nrp_id": "WP_012855507.1", "uniref100_id": "UniRef100_D1J817", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21EB", "RefSeq:WP_012855507.1", "UniRef:UniRef100_D1J817" ] }, "ips": { "uniref100_id": "UniRef100_D1J817", "uniref90_id": "UniRef90_D1J817" }, "psc": { "uniref90_id": "UniRef90_D1J817", "gene": "MraZ", "product": "Transcriptional regulator MraZ", "uniref50_id": "UniRef50_Q6KHR3", "cog_id": "COG2001", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_Q6KHR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR3" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "DFOKDJOJIG_581", "locus": "GCA013367855_00558" }, { "type": "tRNA", "sequence": "contig_1", "start": 315616, "stop": 315691, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 79.9, "nt": "GCTAGTTTAGCTCAGTTGGTAGAGCAATTGATTCGTAATCAATGGGTCGGGGGTTCGATCCCTCTAACTAGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 315649, 315651 ], "id": "DFOKDJOJIG_16", "locus": "GCA013367855_00559" }, { "type": "tRNA", "sequence": "contig_1", "start": 315782, "stop": 315856, "strand": "+", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 77.6, "nt": "GCCGACTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTTGTCGTTGGTTCAATCCCAATAGTCGGCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 315814, 315816 ], "id": "DFOKDJOJIG_17", "locus": "GCA013367855_00560" }, { "type": "tRNA", "sequence": "contig_1", "start": 315861, "stop": 315936, "strand": "+", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 66.5, "nt": "GGCCCTATGGTGAAGCGGTTAACACATATGGTTTTCATCCATACATTCGTGGGTTCAAGTCCCGCTAGGGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 315894, 315896 ], "id": "DFOKDJOJIG_18", "locus": "GCA013367855_00561" }, { "type": "tRNA", "sequence": "contig_1", "start": 315939, "stop": 316014, "strand": "+", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 82.1, "nt": "GGAGAATTAGCTCAGCTGGGAGAGCATTGCCCTTACAAGGCAAGGGTCGTGGGTTCGAGCCCCTCATTCTCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 315972, 315974 ], "id": "DFOKDJOJIG_19", "locus": "GCA013367855_00562" }, { "type": "cds", "sequence": "contig_1", "start": 316139, "stop": 317143, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280AD9DE", "UniRef:UniRef90_UPI00280AD9DE" ], "nt": "TTGGTTATATTAATTGTTACTGTAACACTTGTATTAAAAGGAAATAATATTCACGTTTATGGGCTTTTCAATACAAATGCAGAACATGGATTTTTAATAAGTTATTTAACACTTATATATAGTTTTTTATCAACACTTCAATCAATGCTTACTATATTAGTTTATGCAAGAATTGGTATTGCATCTGCCGTTAGGGTTAATACACTTTTGAGTTTACCAAACACACATATAAAAAACCACTCAAAGGGAATTGTGATAGAAAATCCCGTTGGAAATATATCATTTGATCATGTATATTTTAGATATAATGAAAATTCGCCTAAATATCAACTAACAGATGCTTCATTTAATGCAAAAGCAGGACAAACTATTGCTTTAGTTGGAGAAACTGGAGCAGGTAAAACTACTATTATAAACTTATTGAATCGTTTTTATGATTATGAAAAAGGTTCAATAAAGATAGATGGAATAGAGTTAAGAGAAATTTCAAAAGACAGTTTAAGAGATTTTACGGCAGTTGTTTTACAAGATAGCTTTATGTTTAATGAAAGTATTTTAAATAACCTTTTAGTAGGAAATCCAAAAGCAACCAAAGATGATGTAATAAGGGCAGCAAAATTAGCATCTGCTCATCATATTATTGAAAAATTAGAACATGGATATGATACTATAATTGATGATAGCACTTCAATATTATCTAAGGGAGAAAAACAACTTCTTTCTATAGCTAGAGCAATATTAGGAAACAAAAAAATCTTAATACTAGATGAAGCAACATCAAACGTGGATTCTAATACAGAAAAAATAATTCAACAAGCATTAAAAGAAAACATCATGGTTGGTAAAACATCAATAGTTATTGCTCACCGTCTAAGTACGATTAAAAATGCTGATTTAATCTTAGTCGTGGATGATGGTCGTATTATTGAACAAGGAACCCATAAGCAATTGCTAGAATTAAATAAAAAATATGCAAGGTTATATTACTCACAATTTGATGATTAA", "aa": "MVILIVTVTLVLKGNNIHVYGLFNTNAEHGFLISYLTLIYSFLSTLQSMLTILVYARIGIASAVRVNTLLSLPNTHIKNHSKGIVIENPVGNISFDHVYFRYNENSPKYQLTDASFNAKAGQTIALVGETGAGKTTIINLLNRFYDYEKGSIKIDGIELREISKDSLRDFTAVVLQDSFMFNESILNNLLVGNPKATKDDVIRAAKLASAHHIIEKLEHGYDTIIDDSTSILSKGEKQLLSIARAILGNKKILILDEATSNVDSNTEKIIQQALKENIMVGKTSIVIAHRLSTIKNADLILVVDDGRIIEQGTHKQLLELNKKYARLYYSQFDD", "aa_hexdigest": "67138204e4166c58d564348c7caaf4cf", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 316139, "stop": 317443, "observations": { "mutation": [ 317167 ], "stop": [ 317167 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280AD9DE", "query_cov": 1.0, "subject_cov": 0.6958333333333333, "identity": 0.988, "score": 627.0, "evalue": 2.64e-224, "gene_start": 1, "gene_end": 334, "reference_start": 147, "reference_end": 480, "reference_sequence": "MIIKNQVILFLFLVNDINSLSETIFKLLNELIQCAINVILSVIFLSMVSLVLSAVVLPVLFLFSFIGILITKKAQKAFIQTRKHFGELNAFVEEMLTNSKIIKTFEQQEQVNNHGKQIARNIFNTAFTGDLNNQIINPWIVFTNNILVILIVTVTLVLKGNNIHVYGLFNTNAEHGFLISYLTLIYSFLSTLQSMLTILVYARIGIASAVRVNTLLSLPSTHIKNHSKGIVIENPVGNITFDHVYFRYNENSPKYQLTDASFNAKAGQTIALVGETGAGKTTIINLLNRFYDYEKGSIKIDGIELREISKDSLRDFTAVVLQDSFMFNESILNNLLVGNPKATKEDVIRAAKLASAHHIIEKLEHGYDTIIDDSTSILSKGEKQLLSIARAILGNKKILILDEATSNVDSNTEKIIQQALKENIMVGKTSIVIAHRLSTIKNADLILVVDDGRIIEQGTHKQLLELNKKYARLYYSQFDD" }, "paralog": false, "identity": 0.9907834101382489, "score": 827.0, "evalue": 2.93e-305, "description": "Internal stop codon at 317167. Nonsense mutation around 317167.", "psc": { "uniref90_id": "UniRef90_UPI00280AD9DE", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00280AD9DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280AD9DE", "UniRef:UniRef50_UPI00280AD9DE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00280AD9DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280AD9DE" ], "product": "ABC transporter ATP-binding protein" } }, "id": "DFOKDJOJIG_582", "locus": "GCA013367855_00563" }, { "type": "cds", "sequence": "contig_1", "start": 317165, "stop": 317866, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGTTTTGTTTTCATTTTTTCATGCAATTTTTTATGCAATAGGTTCATTTTTAATTGGATATATTTTTACTATATGTTTAACAAAAGATGTATTGGATCATAAAACACCTTTTAATTTAAAATTATTTATAGGCATGGCTTCATGTTTGGTTATATCGTTTATATTGTATGGAATTTTTAGATATGTTTCAGGAGTATTCTTTGTAAAAATAACTTTTTCAGTTTGCGAGAATATAAGAAAAACCGCCATGAAAAACCTTTTATATATTCCTGTTTCGTATTTTGATAATCAAAAATCAGGTAATATTATTTCTATTTTAGTAAATGATATTAATAGTTTATCTGAAACTATATTTAAACTATTAAATGAATTAATCCAATGTGCTATAAATGTTATTTTATCTGTAATATTTTTATCAATGGTTAGCTTAGTTTTATCTGCTGTTGTTTTACCTGTTTTATTCTTATTTTCATTTATAGGAATATTAATTACTAAAAAAGCTCAAAAAGCATTTATTCAAACTAGAAAACATTTTGGGGAATTAAACGCTTTTGTTGAAGAAATGCTAACTAATTCTAAAATTATTAAAACTTTTGAACAACAAGAACAAGTTAATAATCATGGTAAACAAATAGCAAGAAACATATTTAACACAGCCTTCACTGGAGATCTTAATAACCAAATAATTAACCCTTGA", "aa": "MAVLFSFFHAIFYAIGSFLIGYIFTICLTKDVLDHKTPFNLKLFIGMASCLVISFILYGIFRYVSGVFFVKITFSVCENIRKTAMKNLLYIPVSYFDNQKSGNIISILVNDINSLSETIFKLLNELIQCAINVILSVIFLSMVSLVLSAVVLPVLFLFSFIGILITKKAQKAFIQTRKHFGELNAFVEEMLTNSKIIKTFEQQEQVNNHGKQIARNIFNTAFTGDLNNQIINP", "aa_hexdigest": "511f8b17b57075d707311b7b34376e9d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26396.00490000002, "isoelectric_point": 9.158330345153807 }, "id": "DFOKDJOJIG_583", "locus": "GCA013367855_00564" }, { "type": "cds", "sequence": "contig_1", "start": 317867, "stop": 317962, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGAACTAAAGAAAAGCAAAAACTTTCATTTATTACTTTGTTTAAATTAGTTTTTAAATATTCAGGTGAATCAAAAACAAAATTTTGA", "aa": "MKKRTKEKQKLSFITLFKLVFKYSGESKTKF", "aa_hexdigest": "9e033a066f30cb864e5c512b3f8fafc4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3770.5296000000008, "isoelectric_point": 10.363437843322753 }, "id": "DFOKDJOJIG_584", "locus": "GCA013367855_00565" }, { "type": "cds", "sequence": "contig_1", "start": 317977, "stop": 318945, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFCB5D" ], "nt": "GTGGTGCTTTATTCATTAGTTGTTCATGATGTTATTTCTAATGTAGCAACATATGATACAATTGTAAGATTTAATATTTTTATTGACTATTCATTTTATGTAACGTTTGGTGTTATATTGGGAGCAGTATTTATTTCTGCATTTGTAAAAGGAAAAATTTCTGCTACTAGAGTTATAGAAGTATTAAATACTAACGAAGATAAAATTTATGTATCAGATGGTAAAAAAATAACAAAAAACTTTGATTTAAAAGTTGAAAATTTAAGTTTTAAATATTATTCTTCAAATCCAAATTATACTTTGCACAATATAAGTTTTAGCCTACCGTTTGGAAAAACTCTTGGATTGGTTGGCCCTACTGGTAGTGGCAAGAGCATACTTGCAAATTTATTGGTTAATAACTATCTATATAATGAAGGATCGATTTTAATTGGGGATAATGAAATTAAAGAAGTTAATACAAAAAATTTACATGAAACGATCGGAATTGTTTACCAAGATGCCCTATTAAATACAGGAACAATAAAAGAAAATATGCTTTTTGCTAAGAATAATGCAACAGATGAAGAAATAAATCAAGCATTACGCGATGCTTGTGCATATGATTTTGTTTATAAATTTGAAGAAAATTTAGATCATGTCATTACTCAAGGGGCAACAAACCTTTCGGGTGGTCAAAAACAACGTCTTTCAATTGCAAGAACTTTATTAACTAAACCAAAAATTTTAATATTAGATAACTCAACTAGTGCTTTAGATAATATTACTGCACACCAAGTTTTACAAAATATAAAAAATAAATATTCATGTTCAACTATAATAATTAGCCAAAAATTGCCATTAATAAAGAATGCTGATGAAATTATTGTTTTATCTGATGGAAACATGTTGGATAAAGGAAATCATGAAGAATTAATGAAATCATGTTCATATTATAATGAATCATATAATAACCAATTAAGCCAATAA", "aa": "MVLYSLVVHDVISNVATYDTIVRFNIFIDYSFYVTFGVILGAVFISAFVKGKISATRVIEVLNTNEDKIYVSDGKKITKNFDLKVENLSFKYYSSNPNYTLHNISFSLPFGKTLGLVGPTGSGKSILANLLVNNYLYNEGSILIGDNEIKEVNTKNLHETIGIVYQDALLNTGTIKENMLFAKNNATDEEINQALRDACAYDFVYKFEENLDHVITQGATNLSGGQKQRLSIARTLLTKPKILILDNSTSALDNITAHQVLQNIKNKYSCSTIIISQKLPLIKNADEIIVLSDGNMLDKGNHEELMKSCSYYNESYNNQLSQ", "aa_hexdigest": "f8b3450cf4ed0b3f827926787973c3d2", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DFCB5D", "query_cov": 0.9440993788819876, "subject_cov": 0.9589905362776026, "identity": 0.523, "score": 317.0, "evalue": 2.36e-104, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021DFCB5D" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFCB5D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFCB5D" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "DFOKDJOJIG_585", "locus": "GCA013367855_00566" }, { "type": "cds", "sequence": "contig_1", "start": 319024, "stop": 319281, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATTGGTGGATGTATTTTAGCCGTCATGAATAACGTAAAAATGGCAATAGGATTAATTTTGTTAGTTCCAATAATTTTAACAATAGTTTCTATTATGGCAAAAAAAGCAAGTCCTTCAATTAAAAAAGCTCAATTAGCTGTTGAAGAAATAACTAAGCAAGTTGATGAAAATATATTGGGATCTCGTTTGATAAAAATATATGATTTATATAAAAATAGAGAAGAAAAGTTTGCAAAAATAAATAAAACATGA", "aa": "MIIGGCILAVMNNVKMAIGLILLVPIILTIVSIMAKKASPSIKKAQLAVEEITKQVDENILGSRLIKIYDLYKNREEKFAKINKT", "aa_hexdigest": "f4b0a80b858dff6d1eaa5909d85d28fe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9426.415499999992, "isoelectric_point": 9.673819541931152 }, "id": "DFOKDJOJIG_586", "locus": "GCA013367855_00567" }, { "type": "cds", "sequence": "contig_1", "start": 319327, "stop": 319719, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGTAGGAAGCTTTATTTCTTTAATTTCAGTTATATTTGAATTGATTACTCCTACATTAATTTCTCAATTCATTAGATTGCTTTTTATTGAAAATAAAAATAGCCAAGTAGATTTAAGCTTTTTTAATGGATTAATAAACTTTACATTTAATAATGCTATTAATGCTCGTAATTATATGATTTATTCTATAATAGTTTTAACTGTAATTTCAATGTCTTTAACATTTGGCTGCACTAGCTTAATTGTATATGCAGCAGAAAGAAGTAGTAGATATATTAGAGAAAACTTTATTAGAAAATTAAATACATTAAGTTTGAAAAATATATCTGATTTGAAACCCGAATCATTAATTACTAGGGTGAGTGATGATATTGCGGTTTTCTGA", "aa": "MFIVGSFISLISVIFELITPTLISQFIRLLFIENKNSQVDLSFFNGLINFTFNNAINARNYMIYSIIVLTVISMSLTFGCTSLIVYAAERSSRYIRENFIRKLNTLSLKNISDLKPESLITRVSDDIAVF", "aa_hexdigest": "7e7c0d2a197818a7cc0aa47384995710", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14833.22609999998, "isoelectric_point": 8.88685398101807 }, "id": "DFOKDJOJIG_587", "locus": "GCA013367855_00568" }, { "type": "cds", "sequence": "contig_1", "start": 319898, "stop": 320797, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGAAGTTGATGATCAATTAAAGAAATTGCAAAATGAAGATACTTCTTCAATGAGCCAAGAACAAAAGCAACTTCATCAACAAAAAATTAGCGAATTACAAAGCAAAAAAAATGAAATTGAAGTTAAAGTTAATGAAGCACAAACAAAATATGACCAAAAGCAAAAAGAACTTCAACCTAAAATTGATAAATTAAATAAAGAAATCGATGAACTTAATAATAATATAGATAAGTTAAATAAAGAATTAGCTTCAAACTTCTATGGAGTAGAAGAAATTATTAATACTTCAACAATTTTTAATTTGAACCCAATTGATATAGTTGATATTTTAAGCAAAGACATTGAAAAAGAAATGATTGAAAAAGGTGATAATTACATTTCGGTTCAAAATTTTGATTATGATAAAACAACATTAAAATTAAAACATTCAATGATTGCGTTTCAGTATATAAAAAATATGGTTGATGTACAAAATGTTTATATCTTTTCATTTCCTGAAATAGAAAATAACAAAGTAAAATCAATTAGAGTTTCTGTAGTTTCTCTTCAAGATTTTCAAAATAATGATTTTAGCAATATGTCATCTAAACGCGTCAATGTTAATAAAGTGGTAAACGAAGATAAAAACTTCATGCAAAAAATAGTTTTAAAAGAACATTTTAGAAAATCAGTAACCGTAAAATACATTGGCGATAAAAAAACAGTTGATGAAGCAATTAAAGATGATATTTCTAAATTTGAATACAAATTACCTGAAGGATTAACATTCGAAAAAATCTTAGTTGAAAAATATACAACCAAAAAAAATAAGAAGGAATATATAAAATATACAATTACTGCTAAATGGAACGGAATAGAAGTTGAATTTAAAAATAAAATTAGTTTAAAAAAATAA", "aa": "MEEVDDQLKKLQNEDTSSMSQEQKQLHQQKISELQSKKNEIEVKVNEAQTKYDQKQKELQPKIDKLNKEIDELNNNIDKLNKELASNFYGVEEIINTSTIFNLNPIDIVDILSKDIEKEMIEKGDNYISVQNFDYDKTTLKLKHSMIAFQYIKNMVDVQNVYIFSFPEIENNKVKSIRVSVVSLQDFQNNDFSNMSSKRVNVNKVVNEDKNFMQKIVLKEHFRKSVTVKYIGDKKTVDEAIKDDISKFEYKLPEGLTFEKILVEKYTTKKNKKEYIKYTITAKWNGIEVEFKNKISLKK", "aa_hexdigest": "68ce729c752876cbdccf7672ff589c05", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35208.77069999997, "isoelectric_point": 7.65600757598877 }, "id": "DFOKDJOJIG_588", "locus": "GCA013367855_00569" }, { "type": "cds", "sequence": "contig_1", "start": 320921, "stop": 321157, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATCTTACTGTTTCATTTAAAGATTATGAAAATAGTACTAAGAAATCAAAATTTGTTGAAATTGATTTAAGCAAATTGGGCGTTGATGAATATAACAAGATTAGAAAACAAAATAATGAAACTGAAATAGATGACCAACAAGCCCCAACATCAACTATTGAAGACCAAGACGTAAAAGAATTAAATAAAGATGCATTCTTAAAATATTTCAAAGAACAAAAGGCTACATGA", "aa": "MINLTVSFKDYENSTKKSKFVEIDLSKLGVDEYNKIRKQNNETEIDDQQAPTSTIEDQDVKELNKDAFLKYFKEQKAT", "aa_hexdigest": "1b1e85847d9f25b23eb87bc4d6c2736a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9138.062299999998, "isoelectric_point": 4.921483802795408 }, "id": "DFOKDJOJIG_589", "locus": "GCA013367855_00570" }, { "type": "cds", "sequence": "contig_1", "start": 321224, "stop": 322141, "strand": "-", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J821", "UniRef:UniRef90_D1J821" ], "nt": "TTGGTAAATCCTGAAAAACCTCTTAGAAGTGGAGACAAGTTAAACTCTAAATTGTTATTAACAAAAGATGAAAAAGGAAACTTTATTTTGAAATATAAGGTTGCAATTTTTAAACAAAATGGTAAACATATTATTTCAAACAAGGTTTATGAAAGCAATTTGGGAAACTTAGATTCTGGATTAGATCAAGTTTCAGAAGAAGATTTTAAAGAAGTTTCAAAAAATCTAGTCTTTGACTATCCTAACAAACAAGAAACATACATAAAAGACGCAGACACATCAAAAGTATTTTTAAAAACTATTAAAGAAAACTTTTCAATTGTTAAAGATTTGTTAAAAATTACTAAAAATACAGGAGAAGATGGAACATATGACTTGTCAATTCTTTACTATTTATCATACAAGAATAAACAAATTTCAAGTCAAAAATACATTTATGAAATAAAAGGTTTTAAACTAACTCCAGAATTGCAAGAAAAAATAAATAAGGCCAAAAAAGAACTAAAAGATCAAATTGAAAAAACAAATGTTTTAGTTGCAAATGCCAAGTCATATCAAGATATTTTAAAAAATAAACAAATAAACAATTTTGATAAAAAACCAAATTTTGCAGTTCAAGGCTACGACCAAGCTTCATATTCAGTAGCATTAAGCAATTTAAAAGTTGAAGATAAAAAAATAACATTGACTTTAACTTTGTCAAGTCTTTTAAATAAAGAAATAAGTGAATCTAAAACTATTGAAGTTAAATTAGATAAATTTGCAAATGGTTTAGATAATATTGCATTGTTATCACCAGAAAAACAAGATGAACTTTTAAACAAAGCTTTAGAAACTTCAATTTATCCATTTTATAGTAAAGACAAATATTACATTTCAAAGAAATCTTTTGACAAATTAGAAGATAAATCATACTGA", "aa": "MVNPEKPLRSGDKLNSKLLLTKDEKGNFILKYKVAIFKQNGKHIISNKVYESNLGNLDSGLDQVSEEDFKEVSKNLVFDYPNKQETYIKDADTSKVFLKTIKENFSIVKDLLKITKNTGEDGTYDLSILYYLSYKNKQISSQKYIYEIKGFKLTPELQEKINKAKKELKDQIEKTNVLVANAKSYQDILKNKQINNFDKKPNFAVQGYDQASYSVALSNLKVEDKKITLTLTLSSLLNKEISESKTIEVKLDKFANGLDNIALLSPEKQDELLNKALETSIYPFYSKDKYYISKKSFDKLEDKSY", "aa_hexdigest": "17676a4f54e4ef0a7d6ee7a2f1c3f6c2", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 320927, "stop": 322441, "observations": { "mutation": [ 321226, 322171, 322315 ], "stop": [ 321226, 322171, 322315 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J821", "query_cov": 1.0, "subject_cov": 0.5222602739726028, "identity": 0.977, "score": 559.0, "evalue": 2.55e-196, "gene_start": 1, "gene_end": 305, "reference_start": 142, "reference_end": 446, "reference_sequence": "MKKHTKILLALGSVATSVFAIPLVAAGCIKTKKPEVKPEEPKKPEGEKPGTTPEVKPEDPSHEEETNKNDADKYGEESISKITWNAENLEFIKKVLNNLDKYKILFERNNKNIMAFEGAYDFKNQEFAIAKWNSKSGVSVQLVNPEKPLRSGDKLNSKLLLTKDEKGNFILKYKVAIFKQNGKHIISNKVYESNLGNLDSGLDQVSEEDFKEVSKNLVFDYPNKQETYIKDADTSKVFLKTIKENFSIVKDSLKITKNTGEDGTYDLSILYYLSYKNKQISSQKYIYEIKGFKLTPELQEKIDKARKELKDQIEKTNVLVANAKSYQDILKNKQINNFDKKPNFAVQGYDQASYSVALSNLKVEDKKITLTLTLSSLLNKEISESKTIEVKLDKFANGLDNIALLSFEKQDELLNKALETSIYPFYSKDKYYISKKSIDKLENKSYWINNKNSEINYTLGELKFEGDKCLINLTVSFKDYENSTKKSKFVEIDLSKLGVDEYNKIRKQNNETEIDDQQAPTSTIEDQDVKELNKDAFLKYFKENKDKLSNDEVKNISTFMFVEKLKNISLLSKDIEKRNDWKRW" }, "paralog": false, "identity": 0.9471624266144814, "score": 934.0, "evalue": 0.0, "description": "Internal stop codon at 321226, 322171, 322315. Nonsense mutation around 321226, 322171, 322315.", "psc": { "uniref90_id": "UniRef90_D1J821", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J821", "UniRef:UniRef50_D1J821" ] }, "pscc": { "uniref50_id": "UniRef50_D1J821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J821" ], "product": "Variable surface lipoprotein" } }, "id": "DFOKDJOJIG_590", "locus": "GCA013367855_00571" }, { "type": "cds", "sequence": "contig_1", "start": 322313, "stop": 322582, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATACAAAAATTTTATTAGCCTTAGGTAGTGTTGCAACCTCGGTTTTTGCAATACCATTAGTGGCAGCAGGTTGCGTAAAAACAAAAAAACCAGAAGTTAAACCAGAAGGTGAAAAACCAGGAACAACACCTGAAGTTAAACCGGAAGATAAACCAGGAACAACACCTGAAGTTAAACCAGAAGATCCAAGTCATGAAGAAGAAACAAATAAAAATGATGCTGATAAATATGGTGAAGAATCAATTTCAAAAATAACATGA", "aa": "MKKHTKILLALGSVATSVFAIPLVAAGCVKTKKPEVKPEGEKPGTTPEVKPEDKPGTTPEVKPEDPSHEEETNKNDADKYGEESISKIT", "aa_hexdigest": "999bf894a52ac79456bb596c5dd5cc7e", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9553.741399999997, "isoelectric_point": 5.698586463928222 }, "id": "DFOKDJOJIG_591", "locus": "GCA013367855_00572" }, { "type": "tRNA", "sequence": "contig_1", "start": 322860, "stop": 322935, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 81.9, "nt": "GCATCCTTAGCTCAGTTGGCAGAGCAAGGGACTCTTAATCCCTGGGTCGTGGGTTCGAGCCCCACAGGATGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 322928, 322930 ], "id": "DFOKDJOJIG_30", "locus": "GCA013367855_00573" }, { "type": "tRNA", "sequence": "contig_1", "start": 323037, "stop": 323121, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 60.8, "nt": "GCGCGAGTGGTGAAATTGGCAGACACGTTAGTTTTAGGCACTAATGCTTTGCGGCGTGAGGGTTCAAGTCCCTCCTCGCGTACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 323091, 323093 ], "id": "DFOKDJOJIG_29", "locus": "GCA013367855_00574" }, { "type": "tRNA", "sequence": "contig_1", "start": 323125, "stop": 323200, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 88.2, "nt": "GCCGTTTTAGCTCAGTTGGCAGAGCAACTGACTTGTAATCAGTAGGTCGTAGGTTCAAGTCCTATAAACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 323165, 323167 ], "id": "DFOKDJOJIG_28", "locus": "GCA013367855_00575" }, { "type": "cds", "sequence": "contig_1", "start": 323409, "stop": 324197, "strand": "+", "frame": 3, "gene": "truB", "product": "tRNA pseudouridine(55) synthase TruB", "db_xrefs": [ "COG:COG0130", "COG:J", "EC:5.4.99.25", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "KEGG:K03177", "SO:0001217", "UniRef:UniRef50_A0A501X9B0", "UniRef:UniRef90_D1J822" ], "nt": "ATGTTTTATAAGTTATATAAAAAAAGAGGCCAATCAAGTTTTTTTGCAATAAAAAAATTTGCAAAAAATAATAATATCAATAAAATAGGACATTGTGGAACATTAGATCCTGAGGCTGAAGGTCTTTTAATCGTAGCAACAGATGAAGATACAAAGGCCTTGTCTTTAATTGAAAATCAAAACAAGGAATACTATGTGGAAGCAAAATTCCATTACTCTTCAGATAGTTTTGATTTAGGAACATATGTAACTAAAATACAAAATTTTCAAATAATAACTAAATCTCAAATTATACAAGCACTAGAAATATTAAAAAAACAAACAAAACAAGTGCCTCCAATTTATAGTGCAAAAAAAATTAATGGAACAAGAGCATACAAACTTGCTAGAGATAATAAAGAATTTAGTTTAAAAGCCAATGATATAAAAATAAATAATTTAGAATTAATCGAATTTAATGAAGTTAATCAATCCATCATCATTAAGGCCAATGTTTCAAAAGGCACATATATTAGATCATTGATTCATGATTTAGCTAATATATGTAACACAGATGCAGTAGTAGTTGTTTTAAAGCGAATTAAAATTGGCAATATATGTTTAAATGGTCAAGAAGAATTTGAAAAAATTACAAACTTAAACGAACTTTTTAATGTAAAATTATTTAAGCTAACCAAAAATGATATTCAAATATTAGCAAACCAAAATTTAAATAGTTGCTACTTTGAATCATTGAAAAATTTTTGCGGAAACATTATTTTTATTTACTTGAATGACATAGTAGGATGA", "aa": "MFYKLYKKRGQSSFFAIKKFAKNNNINKIGHCGTLDPEAEGLLIVATDEDTKALSLIENQNKEYYVEAKFHYSSDSFDLGTYVTKIQNFQIITKSQIIQALEILKKQTKQVPPIYSAKKINGTRAYKLARDNKEFSLKANDIKINNLELIEFNEVNQSIIIKANVSKGTYIRSLIHDLANICNTDAVVVVLKRIKIGNICLNGQEEFEKITNLNELFNVKLFKLTKNDIQILANQNLNSCYFESLKNFCGNIIFIYLNDIVG", "aa_hexdigest": "df86a3c8e5bf1315d858d9afce9ed2b6", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J822", "query_cov": 1.0, "subject_cov": 0.9128919860627178, "identity": 0.9890000000000001, "score": 502.0, "evalue": 4.08e-179, "valid": true, "gene": "truB", "product": "tRNA pseudouridine(55) synthase TruB", "ec_ids": [ "5.4.99.25" ], "uniref50_id": "UniRef50_A0A501X9B0", "cog_id": "COG0130", "cog_category": "J", "kegg_orthology_id": "K03177", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501X9B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X9B0" ], "product": "tRNA pseudouridine synthase B" }, "genes": [ "truB" ], "id": "DFOKDJOJIG_592", "locus": "GCA013367855_00576" }, { "type": "cds", "sequence": "contig_1", "start": 324281, "stop": 324652, "strand": "+", "frame": 2, "gene": "cof", "product": "YcsE-related riboflavin metabolism phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_D1J823", "UniRef:UniRef90_D1J823" ], "nt": "ATGACAAAATACAAAATGATAGTATTTGATATTGATGGAACATTATTGCCATTTGGTGTTACTCAATTAACACCAAGAATGCAAGAAATGTTTGAGAAATTAAAAGAACAAGGCTATGTAAATGTGTTGTGTTCTGGTCGTGATATTTTTACAGTAGGCGCCCAAATTGATAACCCTTATGTTGATTATTTTGTAGGTGCAAATGGAACTTTTATTCTTGATCTAAGAACAAAAGAATATATTTTTGAAAAAACTATTAATTACAAAGATTTTATTGAATTTAGAAAATATGCAAGCGAACATAAATTATCTTTTTCTTTTGTTGGAAACAAATGGGGATATTACAATGAATTATTTAATATAGATCATTGA", "aa": "MTKYKMIVFDIDGTLLPFGVTQLTPRMQEMFEKLKEQGYVNVLCSGRDIFTVGAQIDNPYVDYFVGANGTFILDLRTKEYIFEKTINYKDFIEFRKYASEHKLSFSFVGNKWGYYNELFNIDH", "aa_hexdigest": "bc5638f7079da19c8c9f97243eae3cb1", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 324281, "stop": 324955, "observations": { "mutation": [ 324650, 324803, 324818 ], "stop": [ 324650, 324803, 324818 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J823", "query_cov": 1.0, "subject_cov": 0.4606741573033708, "identity": 1.0, "score": 257.0, "evalue": 4.9e-85, "gene_start": 1, "gene_end": 123, "reference_start": 1, "reference_end": 123, "reference_sequence": "MTKYKMIVFDIDGTLLPFGVTQLTPRMQEMFEKLKEQGYVNVLCSGRDIFTVGAQIDNPYVDYFVGANGTFILDLRTKEYIFEKTINYKDFIEFRKYASEHKLSFSFVGNKWGYYNELFNIDHWFYRPYKDKFIPEKEFESQHDKNYLITISTPTPSIVAADLEKFFKEKNLDLWILAQWNGGIFISAKGITKAVGLKILGDLLNISLDEMVAFGDSENDIEMLEKVGLGIAMGNGEDVTKDAAKEICLPVTEDGPYWKLRDKGFFD" }, "paralog": false, "identity": 0.9866071428571429, "score": 444.0, "evalue": 4.51e-161, "description": "Internal stop codon at 324650, 324803, 324818. Nonsense mutation around 324650, 324803, 324818.", "psc": { "uniref90_id": "UniRef90_D1J823", "gene": "Cof", "product": "YcsE-related riboflavin metabolism phosphatase", "uniref50_id": "UniRef50_D1J823", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J823", "UniRef:UniRef50_D1J823", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_D1J823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J823" ], "product": "Cof-type HAD-IIB family hydrolase" } }, "id": "DFOKDJOJIG_593", "locus": "GCA013367855_00577" }, { "type": "cds", "sequence": "contig_1", "start": 324911, "stop": 325054, "strand": "+", "frame": 2, "gene": null, "product": "Hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W0C783" ], "nt": "ATGGTGGCATTTGGTGATTCAGAAAATGATATTGAAATGCTTGAAAAAGTGGGATTAGGTATTGCTATGGGCAACGGAGAAGATGTTACTAAAGACGCTGCAAAAGAAATTTGCTTGCCAGTTACTGAAGACGGCCCATATTGA", "aa": "MVAFGDSENDIEMLEKVGLGIAMGNGEDVTKDAAKEICLPVTEDGPY", "aa_hexdigest": "784c32efb727be907a81c528c83ef89b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W0C783", "query_cov": 0.9361702127659575, "subject_cov": 0.8, "identity": 0.568, "score": 52.8, "evalue": 3.3e-08, "valid": false, "product": "Hydrolase", "uniref50_id": "UniRef50_A0A2W0C783" }, "pscc": { "uniref50_id": "UniRef50_A0A2W0C783", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W0C783" ], "product": "Hydrolase" }, "genes": [], "id": "DFOKDJOJIG_594", "locus": "GCA013367855_00578" }, { "type": "cds", "sequence": "contig_1", "start": 325154, "stop": 325879, "strand": "+", "frame": 2, "gene": "spoU", "product": "tRNA/rRNA methyltransferase", "db_xrefs": [ "COG:COG0566", "COG:J", "RefSeq:WP_020002407.1", "SO:0001217", "UniParc:UPI000371E724", "UniRef:UniRef100_A0A6A8PZD2", "UniRef:UniRef50_A0A3B0PPX9", "UniRef:UniRef90_D1J824" ], "nt": "ATGGCAATTACAAGTACACAAGATCCAAAAATTTTGAAATATAAAAAATTACTTACCCCCAAAGGCAGACAAGAACAACAATTGTTTATTGTTGAAGGGTATCATTTAGTAAAAGAAGCAATCGAAGCAAATATAGTTATTGACATTTTAGAATTCAATGACATACTAACTTATAAAAACTCAACTCAAGTAACTAGAGCAATTATTAAAACATTATCAACAACGCAAACACCTCAAAATATCATTGCAATTTGTAAAATACAAAATTTAAATGTTAAAGCAAACAAAATAATAGCCCTTAATAAATTACAAGATCCCGGAAATATTGGTACTATTTTTCGACTTGCTAAATCTTTTGGCTTTGATAGTGTTATAGTTGAAAATATAGATATATACAATGAAAAGATAATTCGTTCATCACAAGGAGCAATGTTTTCACTAAACATTATTAAGACAAATGATTTAAGCAAAGAACTGTCAGCCTTTAAAAAAGATGATTTTTTAGTTTATAGTACAACATTAAATAAAAATTCAATTAAGCTCAATAACATTGATTTTAGAAATAAAAAAATTGTTATTGTTTTAGGAAATGAAGGAAATGGTATAGACAAAAAAATTATTGACTTAAGTGATTTTAATGTTTACGTTCCTATTGAATTTGAAAGTCTCAATGTTGCTTGCTGTGGCGCAATTATTATGAATAAAATTTATAATGGAGATAAATAA", "aa": "MAITSTQDPKILKYKKLLTPKGRQEQQLFIVEGYHLVKEAIEANIVIDILEFNDILTYKNSTQVTRAIIKTLSTTQTPQNIIAICKIQNLNVKANKIIALNKLQDPGNIGTIFRLAKSFGFDSVIVENIDIYNEKIIRSSQGAMFSLNIIKTNDLSKELSAFKKDDFLVYSTTLNKNSIKLNNIDFRNKKIVIVLGNEGNGIDKKIIDLSDFNVYVPIEFESLNVACCGAIIMNKIYNGDK", "aa_hexdigest": "546a242d28e326058dd68fc18d0d0209", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000371E724", "ncbi_nrp_id": "WP_020002407.1", "uniref100_id": "UniRef100_A0A6A8PZD2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000371E724", "RefSeq:WP_020002407.1", "UniRef:UniRef100_A0A6A8PZD2" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PZD2", "uniref90_id": "UniRef90_D1J824" }, "psc": { "uniref90_id": "UniRef90_D1J824", "gene": "SpoU", "product": "tRNA/rRNA methyltransferase", "uniref50_id": "UniRef50_A0A3B0PPX9", "cog_id": "COG0566", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PPX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PPX9" ], "product": "tRNA/rRNA methyltransferase" }, "genes": [ "spoU" ], "id": "DFOKDJOJIG_595", "locus": "GCA013367855_00579" }, { "type": "cds", "sequence": "contig_1", "start": 326038, "stop": 326511, "strand": "+", "frame": 1, "gene": "ribF", "product": "FAD synthase", "db_xrefs": [ "COG:COG0196", "COG:H", "EC:2.7.1.26", "EC:2.7.7.2", "KEGG:K11753", "SO:0001217", "UniRef:UniRef50_A0A448ZYJ6", "UniRef:UniRef90_D1J825" ], "nt": "ATGATGTTTAAAAATCCAGTTAAAAATTCTCAGGTATTAACAAAAAAAGCAATGCAACCAAAAACCAGATTATATACATTAGCAATGTTGGGATTTGATTATGTTTTTTTAGTTGATGTAAACCAAACGATATTGAATTGTAGTTATAGCGATTTTATTCAAGGATTAAAGATAAATAATGTTAAAAATGTTGTTTGTGGTCAAGATTTTAGGTTTGGGTTCAATAGAGAGGGTAACATTGATAAATTAAAAAAATATTTCAATGTATATGTTGCCAATGAATTAAAGGTAAATAAACAAAAAATTTCTTCAACAATAATCAATGAATTTATTGAAGAAGGAAACATAAATGGCTTGAACAAATTACTAATAGGCAAATATTCAATAATTGTAAATTCTCAAAAATTTAATTTTAAATATCCAGAACATTTAAATCAATTAAAAGCAGGAATTTATATTGCCAATGTCGTTTGA", "aa": "MMFKNPVKNSQVLTKKAMQPKTRLYTLAMLGFDYVFLVDVNQTILNCSYSDFIQGLKINNVKNVVCGQDFRFGFNREGNIDKLKKYFNVYVANELKVNKQKISSTIINEFIEEGNINGLNKLLIGKYSIIVNSQKFNFKYPEHLNQLKAGIYIANVV", "aa_hexdigest": "5fff10a87e67d218653ed19fe78cbec9", "start_type": "ATG", "rbs_motif": null, "genes": [ "ribF" ], "truncated": "both", "pseudogene": { "start": 325879, "stop": 326751, "observations": { "mutation": [ 325894, 326509 ], "stop": [ 325894, 326509 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J825", "query_cov": 1.0, "subject_cov": 0.5358361774744027, "identity": 1.0, "score": 310.0, "evalue": 9.81e-105, "gene_start": 1, "gene_end": 157, "reference_start": 54, "reference_end": 210, "reference_sequence": "MQVFDWDFKAKLNINEPLTICLGSFETLHLGHNELFKIAKDLKQSYPDQKLAIMMFKNPVKNSQVLTKKAMQPKTRLYTLAMLGFDYVFLVDVNQTILNCSYSDFIQGLKINNVKNVVCGQDFRFGFNREGNIDKLKKYFNVYVANELKVNKQKISSTIINEFIEEGNINGLNKLLIGKYSIIVNSQKFNFKYPEHLNQLKAGIYIANVVWNNIEYHGLIFINKNQFDSNNNLQENNIIYLFDIDYFISKYQELYIEFLQCIRHINNNSENQISTNDIEIGKKYFISKEKMNS" }, "paralog": false, "identity": 0.9758620689655172, "score": 570.0, "evalue": 9.93e-210, "description": "Internal stop codon at 325894, 326509. Nonsense mutation around 325894, 326509.", "psc": { "uniref90_id": "UniRef90_D1J825", "gene": "RibF", "product": "FAD synthase", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_A0A448ZYJ6", "cog_id": "COG0196", "cog_category": "H", "kegg_orthology_id": "K11753", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J825", "EC:2.7.1.26", "EC:2.7.7.2", "UniRef:UniRef50_A0A448ZYJ6", "COG:COG0196", "COG:H", "KEGG:K11753" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ6" ], "product": "FAD synthase" } }, "id": "DFOKDJOJIG_596", "locus": "GCA013367855_00580" }, { "type": "cds", "sequence": "contig_1", "start": 326815, "stop": 327327, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATGGCTATGAAATTGTTAAACAAATGTTTAATTATGGCCATACTTATAGAGAAAAAGAAATCAATAAAATGATAAAAGATATATTCGTGAACCTACCTTTAAATAAAGACGAATATACTTATAAATTTCTTTGTGAAAATTCTATAAATTATTTTGTTAATAATAAAATGTTAGAAAAATTAATAAATCCTGATGGTGAAATCTTTTATCATGATGTTTCAAAGCCAAATACTTTTAGAATGCCAAAGGATTGTGACAATAGAAAAATATCAGAAATAAGTTCGTTGGAAATGTTTGATTTAATAAAACAAGTTTTAAACAATTCAAAAAGTTCTATTTTAAAAAACAATTTAACTAATACTATTTTAAAAACATTACTAATAGCAAGAACAGAAAATAATATTTCAAAATTAAATGATCTATATGGATATTTAAATAATTTAGGGTTAATTAAAGTTATTTCAAACGGTGATATTGAATATATTGACTTGAACAACAAAGATTAA", "aa": "MLNGYEIVKQMFNYGHTYREKEINKMIKDIFVNLPLNKDEYTYKFLCENSINYFVNNKMLEKLINPDGEIFYHDVSKPNTFRMPKDCDNRKISEISSLEMFDLIKQVLNNSKSSILKNNLTNTILKTLLIARTENNISKLNDLYGYLNNLGLIKVISNGDIEYIDLNNKD", "aa_hexdigest": "ddf1cb190413137f1a10e3079c24a542", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19949.70890000002, "isoelectric_point": 6.740047264099122 }, "id": "DFOKDJOJIG_597", "locus": "GCA013367855_00581" }, { "type": "cds", "sequence": "contig_1", "start": 327580, "stop": 328452, "strand": "-", "frame": 1, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FAB5C6", "UniRef:UniRef90_UPI001F43A718" ], "nt": "TTGCCTTCAAATAAGTTCGTTTATGATAATAAATTAATTACTTTTCCAACATATCAAAAAACCGCCAAGAAAGACGGGGAATTGCTTCCTTTTGAAGGTGTTTCATTTATTAAAGTCAATGGAATTTACGAAGATCAAACAAACGAAATTGAAGCAAATAGAATCATTGAATTAATAAAGCAATTAAAAGATTATTATGGCAATACAAAAACATTTGGGGTCGTAACGTTTAATTTAAAACAAGCTAACTTAATTGATCACAAATTATTCTTATTTATGAATGAAAATCCTGAATATATGGATTTAGACACAGAAAATAAAATTTTTGTTAAGAACATTGAAAGTGTTCAAGGTGATGAAAGAGACATTATTATTTTAGGAATTACAAATAGTTGGGATAAAAACGGAAGATTGTCAAGATTCTTTGGACCATTAAATAATGTTGGCGGTGAAAAGAGACTAAACGTCGCAATAAGTAGAGCCAAAGAAGCAACTATTGTTGTATCTTCAATATCATATTCTGATTTAAAAGTAGACGATTTAAAAAATGAAGGACTAAGATTTTTAAAGCAATATTTACAATTTGCAGAATTTGGTTACGACAGCAAATCAGATGATATATTACAAGATGACACTAAAACATTTGATAGTGAGTTTGAAATCCAAGTTTATGATGAACTTAAGAAGCTTGGATATAAAGTAAATACTCAAGTAGGATGTTCAAAATTCAAAATAGATTTAGCAATTGTTGATGATAAAAATCCATCGAAATATATTTTAGGTATTGAATGCGATGGCGCCACATATCATTCATCAAAAAGCGCTCGTGATAGAGATGCGATTAGACAAATGCTACTTGAGTCAAGAGGTTGA", "aa": "MPSNKFVYDNKLITFPTYQKTAKKDGELLPFEGVSFIKVNGIYEDQTNEIEANRIIELIKQLKDYYGNTKTFGVVTFNLKQANLIDHKLFLFMNENPEYMDLDTENKIFVKNIESVQGDERDIIILGITNSWDKNGRLSRFFGPLNNVGGEKRLNVAISRAKEATIVVSSISYSDLKVDDLKNEGLRFLKQYLQFAEFGYDSKSDDILQDDTKTFDSEFEIQVYDELKKLGYKVNTQVGCSKFKIDLAIVDDKNPSKYILGIECDGATYHSSKSARDRDAIRQMLLESRG", "aa_hexdigest": "10d5851d9578eb2116f03c9dca30e617", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 327277, "stop": 328425, "observations": { "mutation": [ 327396, 327552, 327564, 327582 ], "stop": [ 327396, 327552, 327564, 327582 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F43A718", "query_cov": 0.9689655172413794, "subject_cov": 0.5072202166064982, "identity": 0.993, "score": 554.0, "evalue": 4.73e-195, "gene_start": 10, "gene_end": 290, "reference_start": 19, "reference_end": 299, "reference_sequence": "MIEKSLYDFATPDFQTLPNKLITFPTYQKTAKKDGQLLPFEGVSFIKVNGIYEDQTNEIEANRIIELIKQLKDYYGNTKTFGVVTFNLKQANLIDHKLFLFMNENPEYMDLDTENKIFVKNIESVQGDERDIIILGITNSWDKNGRLSRFFGPLNNVGGEKRLNVAISRAKEATIVVSSISYSDLKVDDLKNEGLRFLKQYLQFAEFGYDSKSDDILQDDTKTFDSEFEIQVYDELKKLGFKVNTQVGCSKFKIDLAIVDDKNPSKYILGIECDGATYHSSKSARDRDAIRQMLLESRGWIIHRIWSLNWFKNKRRELEKIVDKVNQIKQKKFVVKQNEYKTIENSQFEVIEKRDNFNFEKWLPFNYSYRKTLNFIEKSNKRNPLLNGYEIVKQMFNYGHTYREKEINKMIKDIFMNLPLNKNEYTYKFLCENSINYFVNNKMLEKLINPDGEIFYHDVSKPNTFRMPKDCDNRKISEISSLEMFDLIKQVLNNSKSSILKNNLTNTILKTLLIARTENNISKLNDLYGYLNNLGLIKVISNGDIEYIDLNNKD" }, "paralog": false, "identity": 0.9790575916230366, "score": 744.0, "evalue": 4.27e-272, "description": "Internal stop codon at 327396, 327552, 327564, 327582. Nonsense mutation around 327396, 327552, 327564, 327582.", "psc": { "uniref90_id": "UniRef90_UPI001F43A718", "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_UPI0002FAB5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F43A718", "UniRef:UniRef50_UPI0002FAB5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002FAB5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FAB5C6" ], "product": "AAA domain-containing protein" } }, "id": "DFOKDJOJIG_598", "locus": "GCA013367855_00582" }, { "type": "cds", "sequence": "contig_1", "start": 328483, "stop": 328920, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGCCTACGTTAAATTCGACAATATACAATGATGAATTAGAAATTTTAAAGAAGGAATATTCAAAAGTTAGAAATAAAAAACCGATTAGAAAACTATTTAAAGAAATTCCGGATTTAATTCAAAATATTAAGCCTTGTTTTATGATGTCTCCTTCTTCGCTAAGTACATATTTAGCGGATAGTAGCATTACATTTGATAGTGTAATTTTTGATGAGGCTTCTCAATTAACGACAGAAAATGCCTTGATATCAATGATTAAGGCTAAACAAATTATTATTGCCGGTGATATAAATCAATTGCCACCAACTAATTTCTTTAAAACTATGTCTAGCGAGCAAGAACCAAACGAAGACGAAGAAATAGTCAATGAAGAAAATGATTATGATTCACTTCTTGAAAGAATGCAAAGTTCATTTAGACAAGTTACATTAAAATGA", "aa": "MPTLNSTIYNDELEILKKEYSKVRNKKPIRKLFKEIPDLIQNIKPCFMMSPSSLSTYLADSSITFDSVIFDEASQLTTENALISMIKAKQIIIAGDINQLPPTNFFKTMSSEQEPNEDEEIVNEENDYDSLLERMQSSFRQVTLK", "aa_hexdigest": "575d584fa3a671f83e892ac99660ba88", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 51, "aa_cov": 0.3448275862068966, "hmm_cov": 0.9803921568627451, "evalue": 1.0751065090603993e-11, "score": 40.6558723449707, "start": 55, "stop": 104 } ], "seq_stats": { "molecular_weight": 16692.83809999999, "isoelectric_point": 4.676507759094237 }, "id": "DFOKDJOJIG_599", "locus": "GCA013367855_00583" }, { "type": "cds", "sequence": "contig_1", "start": 329023, "stop": 329721, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATCTCGAAATAATATTTTTAAGCGTCTTTTTTCAAAGAGATATAGAGCAATAAAAAAAGAATTAAAAATTAATTTTATATCTTCTAATTTAAATTACAAAAACTTGTTAAAAGATTTAGAGGCATTAAATAACATTCAACTTGCTAAAAATGCAATTACGCTTTTAACAAATAAAATTCCTTTTTCATTTAAAGAAAACTATAATGAAATGGATCTTAATAGTTTAAAGCAACAATTAGATACATTTTCTAAATACGAAATTATTAATAATCAATTAAGTGAATTAAAATTTGATCAAAATTTAAAAATTGGCTTTTTTAACTTTTTATTAAGCCATATTCAATTAACAAATAATTACATAAATTCATATAAAAATTTAATGACTTTTGAAGATAAATTCAATGACTACTTTAATAATAAATATGTAGATTATTCAAATTCATATTATAAAGTTAATGATTTTATAAAAAAACTAAATTTTTTAATTGATTCTGATGAATATCCTGAAGCATATTGTAGTTATATAAAATCAAGACAAGAATTAAAAGACAATCCCTTTACTAATGATTTTGTGGATGCTATTGAATTGCTAGATATTGATTGTTTAAAACAAAATAGTATTGAAAATATTTTTAATAAAAGATTTTATCAACTTTTAATTGATTACTATTTATCAATTGATTTAGCAGATTGA", "aa": "MKSRNNIFKRLFSKRYRAIKKELKINFISSNLNYKNLLKDLEALNNIQLAKNAITLLTNKIPFSFKENYNEMDLNSLKQQLDTFSKYEIINNQLSELKFDQNLKIGFFNFLLSHIQLTNNYINSYKNLMTFEDKFNDYFNNKYVDYSNSYYKVNDFIKKLNFLIDSDEYPEAYCSYIKSRQELKDNPFTNDFVDAIELLDIDCLKQNSIENIFNKRFYQLLIDYYLSIDLAD", "aa_hexdigest": "59108ccf6c4e96003fdcb3fbe0c4d57e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27893.39420000003, "isoelectric_point": 7.595303535461427 }, "id": "DFOKDJOJIG_600", "locus": "GCA013367855_00584" }, { "type": "cds", "sequence": "contig_1", "start": 329782, "stop": 330078, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGTAAAGCTTCAAGATTTAGAAAACATTAAAGAGATTTCTAGCCATTTAATTAATGAAATTCAAAGCCTAATCAATTTCTTAGACCTTGAAATTCCAAGCAATTATCAGCCGAATATTAAAAATAGCAATTATTCATTATTTAATTTATTTTTTAAAATTTTTTCAAATATACAAGATATAGATATAAATCCAAAATATTTAGAAATTTTTTCATATAACAAAACTAATGCAACACAAACCCTAAATGAAATACTATTGCAAAAAGAAAAAATTCAACCTGATATTGACTGA", "aa": "MDVKLQDLENIKEISSHLINEIQSLINFLDLEIPSNYQPNIKNSNYSLFNLFFKIFSNIQDIDINPKYLEIFSYNKTNATQTLNEILLQKEKIQPDID", "aa_hexdigest": "537aa2333e9de85f04aef524c706f4b3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11550.967599999985, "isoelectric_point": 4.582098197937013 }, "id": "DFOKDJOJIG_601", "locus": "GCA013367855_00585" }, { "type": "cds", "sequence": "contig_1", "start": 330094, "stop": 330849, "strand": "-", "frame": 1, "gene": null, "product": "DUF4011 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114E65", "UniRef:UniRef90_UPI0024114E65" ], "nt": "ATGGACATTTATTTAGATGTTGATAAAAACAAAGATAATATCATTAATTCAGAAATTATGAATGCAATGACAGGGATTAACAATGTTTTTAGTTCTGAATATTTGTATTCTGAAGAAGAAGTTGATAATATAGTTTCTCCTAAAGGCTATTATCACTGCCTAATGACTAATTCTTCGCAAGAAGCAGCTATCCAATCAGCAATTAAAGGAAACAGTTTTATTATTCAAGGTCCTCCAGGAACTGGAAAGTCTCAAACTATAGCAAATATAATAAGTGAATTAATTGCAAGAAATAAAAAGGTTCTATTTGTTGCTGAAAAAAAGGCTGCTTTGGATGTTGTTTATAAATCGATCCAAAATCTTGGATTTGCTAATTTTATTTTACTTATTCATAGCAATAACAGCAATAAAAAAGATTTTTCTAATGATTTGTATGAAACATTGCAAGACGGTCAAAAATTTAAAAAAGTAAGTGACGAAGTTATTACTAATAATAATTACTTATATAGCGACTCATTAGAAAAAATGAACGATTTTGTTAGAAAGATATTATCAAATAGAAAACCATTAGGTCGCTCATTATATTTAATGATTGGTGATTATCAAAAATTACTTGCCAATACAAAAGGTATAAATTTTGATATTAAAGATTTTGAAAAAATGGATAATGCACAGTTTGTTGAAATTCAAAACAGTTTAAATAAGTTTAATTTAAACTATAAAAATATAAATTTTAACGCTAAGGAAAGTTTATGA", "aa": "MDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEYLYSEEEVDNIVSPKGYYHCLMTNSSQEAAIQSAIKGNSFIIQGPPGTGKSQTIANIISELIARNKKVLFVAEKKAALDVVYKSIQNLGFANFILLIHSNNSNKKDFSNDLYETLQDGQKFKKVSDEVITNNNYLYSDSLEKMNDFVRKILSNRKPLGRSLYLMIGDYQKLLANTKGINFDIKDFEKMDNAQFVEIQNSLNKFNLNYKNINFNAKESL", "aa_hexdigest": "39629559e00fb6a01beec832814fb647", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 329791, "stop": 331149, "observations": { "mutation": [ 330096, 330897 ], "stop": [ 330096, 330897 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024114E65", "query_cov": 1.0, "subject_cov": 0.4009584664536741, "identity": 0.96, "score": 470.0, "evalue": 1.22e-161, "gene_start": 1, "gene_end": 251, "reference_start": 229, "reference_end": 479, "reference_sequence": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSLLQRKQKTLLEEQGINILYLAFGGLQWKDKTEKNVYSPLLFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNAEGSLYDRYSQYIEFINQKINSFNDSIEQNWKIVDEASLSAFRFSSMDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEFLYSEEEVDNIVSPKGYYHCLMTNSSQEAAIQSAIKGNSFIIQGPPGTGKSQTIANIISELIARNKKILFVAEKKAALDVVYKSIQNLGFANFILLIHSNNSNKKDFSNDLYETLQDGQKFKKVSDEVIANNDYLYSDSLEKMNDFVRKILSNRKPLGRSLYLMIGDYQKLLANTKGINFDIRDFEKIDNEQFVKIQSSLNRFNLNYKNINFNAKESLWYGLSILDVKLQDLENIKEISSHLINEIQSLINFLDLEIPNNYQPYIKNSNYSLFNSFFKIFSNIQDIDINPKYLEIFSYNKTNATQTLNEILLQKEKIQPDIDWILSRYNQNIFNSEIYKNLDFVKSRNNIFKRIFSKRYRAIKKN" }, "paralog": false, "identity": 0.9623893805309734, "score": 853.0, "evalue": 7.79e-315, "description": "Internal stop codon at 330096, 330897. Nonsense mutation around 330096, 330897.", "psc": { "uniref90_id": "UniRef90_UPI0024114E65", "product": "DUF4011 domain-containing protein", "uniref50_id": "UniRef50_UPI0024114E65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024114E65", "UniRef:UniRef50_UPI0024114E65" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024114E65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114E65" ], "product": "DUF4011 domain-containing protein" } }, "id": "DFOKDJOJIG_602", "locus": "GCA013367855_00586" }, { "type": "cds", "sequence": "contig_1", "start": 330895, "stop": 331137, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTACCTGTTTCAATTGAAAGCGAAAATGGATTGCAAAATAAAAAAATAATTATTCAAAGCAATGATTTTGTTGTAAATAATTCATTGATAAAGAAATTAAATGATGATTTTGGACTTGATATTAAATTTGAAGCATTTGATAATGCTGAAGGTTCTCCATATGATAGATATTCACAATACATTGAGTTTATAAATCAAAAAATAAATTCCTTCAATGATTCTATAAAACAAAATTGA", "aa": "MFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNAEGSPYDRYSQYIEFINQKINSFNDSIKQN", "aa_hexdigest": "05486118de694ee7ed56c6451f93df31", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9268.211199999996, "isoelectric_point": 4.5470853805542 }, "id": "DFOKDJOJIG_603", "locus": "GCA013367855_00587" }, { "type": "cds", "sequence": "contig_1", "start": 331174, "stop": 331533, "strand": "-", "frame": 1, "gene": null, "product": "DUF4011 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BA0F4", "UniRef:UniRef90_UPI00280BA0F4" ], "nt": "ATGGAAAATAAAGAATTAATTACAAAAACTTTGGCATATGAGTCTTTAAAGAATAAGCTATTAGACACAACTTTAAGAAATATATTGATAAATTATAAAAAAAATAAATCATCAACTATAACAATTGAAAAAGAAGACATAAAATCAATTTTAGATAAAATTGAAAATAGAAGTGAATTTAAATTTATTGGTTCTCAAGATTCTGATGATGACATCGAAGTTGAAAGCAATGAATTTTTGTCCTCTGCTTCTCAAAAAGAATTATTAGCAATATTGTCGTCATTGCAAAGAAAGCAAAAGACTTTATTAGAAGAACAAGGAATTAATATTCTATATTTGGCTTTTGGTGGGCTTCATTGA", "aa": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSSLQRKQKTLLEEQGINILYLAFGGLH", "aa_hexdigest": "9a2ef1a66a04dfd682a9d49131fd6a47", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 330871, "stop": 331533, "observations": { "mutation": [ 330897, 331176 ], "stop": [ 330897, 331176 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280BA0F4", "query_cov": 0.9915966386554622, "subject_cov": 0.44360902255639095, "identity": 0.983, "score": 208.0, "evalue": 1.1e-65, "gene_start": 1, "gene_end": 118, "reference_start": 1, "reference_end": 118, "reference_sequence": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSLLQRKQKTLLEEQGINILYLSFGGLQWKDKTEKNVYSPLLFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNAEGSLYDRYSQYIEFINQKINSFNDSIEQDWKIIDEASLSAFRFSSMDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEYLYSE" }, "paralog": false, "identity": 0.9590909090909091, "score": 404.0, "evalue": 3.54e-145, "description": "Internal stop codon at 330897, 331176. Nonsense mutation around 330897, 331176.", "psc": { "uniref90_id": "UniRef90_UPI00280BA0F4", "product": "DUF4011 domain-containing protein", "uniref50_id": "UniRef50_UPI00280BA0F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280BA0F4", "UniRef:UniRef50_UPI00280BA0F4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00280BA0F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BA0F4" ], "product": "DUF4011 domain-containing protein" } }, "id": "DFOKDJOJIG_604", "locus": "GCA013367855_00588" }, { "type": "cds", "sequence": "contig_1", "start": 332396, "stop": 333127, "strand": "-", "frame": 3, "gene": "tpiA", "product": "Triosephosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "KEGG:K01803", "RefSeq:WP_036432897.1", "SO:0001217", "UniParc:UPI0005138FF4", "UniRef:UniRef100_A0A454C9S4", "UniRef:UniRef50_D1J827", "UniRef:UniRef90_D1J827" ], "nt": "ATGAAATATTTAATTGGTAATTTAAAAATGAATCTATCTTATTTAGAAAGTCAGAATTATTTAAATGAACTGCAATCATTAAATAAAATTAAAAATTTTAAAAATGTAATAGTTGGAGCGGCTTTGTCATTCGATGCAATGTCTCTTTCTTTACAATATAATAAAAGAAATTTCTTGTTTGGAGTTCAAAATATTTTTTATAAAGAAAAAGGTGCATACACTGGGGAAGTTTCATTTAGATCAGCAGTTGAATTGAACATTGACTTTGTTTTAATAGGACATAGTGAACGTAGAAAATTATTTAATGAAACTGATCAATTGATTAATTTAAAAATTAAACAAATTGAAAAAACTAGCATTATTCCTATTTTGTGTATAGGGGAAGATCAAGAAAATTGTAGCGATAAAGTCTTTGAGGATATTATAAAAAACCAAATATTAAAGGCTTTAGAAGATGTTGATGTTTATAATCGTTTAATTATTTCATACGAACCTACTTATTGCATTGGAAATGGAAAAATTCCTCAAAATAACCATATCGAAAAAGCAATATCTGTAATTAAAAAAATTACTAATAATCAAGTTCCAGTATTGTATGGTGGTTCAGTTTCACAAAAAAATATTGAAAAACTGCTTGAAATTAAGGATTTGGATGGCTTTTTAGTTGGTGGAGCAGCACTGGATGCTCAAGGTTTTTTAGAAATGGGTTCGATAATAGAAAAAAGCAACTAA", "aa": "MKYLIGNLKMNLSYLESQNYLNELQSLNKIKNFKNVIVGAALSFDAMSLSLQYNKRNFLFGVQNIFYKEKGAYTGEVSFRSAVELNIDFVLIGHSERRKLFNETDQLINLKIKQIEKTSIIPILCIGEDQENCSDKVFEDIIKNQILKALEDVDVYNRLIISYEPTYCIGNGKIPQNNHIEKAISVIKKITNNQVPVLYGGSVSQKNIEKLLEIKDLDGFLVGGAALDAQGFLEMGSIIEKSN", "aa_hexdigest": "f072e141c61a95602ea3d9cf508ba5ec", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0005138FF4", "ncbi_nrp_id": "WP_036432897.1", "uniref100_id": "UniRef100_A0A454C9S4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005138FF4", "RefSeq:WP_036432897.1", "UniRef:UniRef100_A0A454C9S4" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9S4", "uniref90_id": "UniRef90_D1J827" }, "psc": { "uniref90_id": "UniRef90_D1J827", "gene": "TpiA", "product": "Triosephosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_D1J827", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803" }, "pscc": { "uniref50_id": "UniRef50_D1J827", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J827" ], "product": "Triosephosphate isomerase" }, "genes": [ "tpiA" ], "id": "DFOKDJOJIG_605", "locus": "GCA013367855_00589" }, { "type": "cds", "sequence": "contig_1", "start": 333145, "stop": 333363, "strand": "-", "frame": 1, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J828", "UniRef:UniRef90_D1J828" ], "nt": "ATGCGTCGCGCAAAAGACAAATTTTATTCAGGAATGACAATTCATCAAAAGGATATTATTATTGCTAAAGAACGTCAAGAATTTGAACGTGAAAGACTCAAAGCTGAAAAGCAAAGAAACTATCAATCAATTAATAATTTATCATTTCTTTTATTAATTTTATATGGATTAATTATATTAATTATATTGATTCCATTTTTGGCATTAAGAATAGTTTGA", "aa": "MRRAKDKFYSGMTIHQKDIIIAKERQEFERERLKAEKQRNYQSINNLSFLLLILYGLIILIILIPFLALRIV", "aa_hexdigest": "1ddc4297115e87bd0db4ed1a055a0b72", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 333145, "stop": 333663, "observations": { "mutation": [ 333408, 333603, 333615 ], "stop": [ 333408, 333603, 333615 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J828", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 1.0, "score": 125.0, "evalue": 5.94e-35, "gene_start": 1, "gene_end": 72, "reference_start": 102, "reference_end": 173, "reference_sequence": "MTLKQKNFRNQKKSISYWKNAWNKATISYFFVSLVIYIALIFIVRYSKKSIDGQYVHSWQNSLTVSMIFAITINFIIVVYRKGMGKWIVNPIANLIRNRIIMRRAKDKFYSGMTIHQKDIIIAKERQEFERERLKAEKQRNYQSINNLSFLLLILYGLIILIILIPFLALRIVW" }, "paralog": false, "identity": 0.9767441860465116, "score": 318.0, "evalue": 1.38e-113, "description": "Internal stop codon at 333408, 333603, 333615. Nonsense mutation around 333408, 333603, 333615.", "psc": { "uniref90_id": "UniRef90_D1J828", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_D1J828", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J828", "UniRef:UniRef50_D1J828" ] }, "pscc": { "uniref50_id": "UniRef50_D1J828", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J828" ], "product": "DUF3899 domain-containing protein" } }, "id": "DFOKDJOJIG_606", "locus": "GCA013367855_00590" }, { "type": "cds", "sequence": "contig_1", "start": 333785, "stop": 333988, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGATGCAGAAATTAATTCAAATAAAATATTTCAAAAACCAGATGGCCAAGATTTTATTGAAAAATTTATAAACCCATATATTAATAAATTAGTTAATAACGAAAAAGACAATTCATATACAAATTTCGAACAAGTTGAGCATGATAAAAGAGAATTTAACGAAATTTATCACAAAAAAATAGATTCACTAATCAAATAA", "aa": "MADAEINSNKIFQKPDGQDFIEKFINPYINKLVNNEKDNSYTNFEQVEHDKREFNEIYHKKIDSLIK", "aa_hexdigest": "f4d61a54993e17ab3a0a386604f05965", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8049.859599999998, "isoelectric_point": 5.250524711608886 }, "id": "DFOKDJOJIG_607", "locus": "GCA013367855_00591" }, { "type": "cds", "sequence": "contig_1", "start": 334088, "stop": 334888, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9N2", "UniRef:UniRef90_A0A454C9N2" ], "nt": "ATGAGAAAAAAATTACTTTATATATTGCCGTTATCTTCAACTATTTTAATAACCCCATTAGTTGCAGCTTCTTGTTATAAAAAGCCTAAGCAACCTAAACAAAATATAGAAACCACACAAACTAAAGATGAAAATGCACAAAAAACTGAAGAATTATTAAAAAAATTTAATGATGCAAAAACTGATCTTTTTAGTTATTTAAAAGATAACTTTTCAACAAGAGAAACTGAACAATTTAACACCTTAAAAGAAGTTCAGGATGCCAAGAAATTATTGGAATCTTTTAATAAAGAATCGCTTGAATCTTCAATAGAAAGTTTAAATGAAAAAATACAAAAAATAAACGTTGCTAAACAAAAAATACTTAAAATTCAACCACTTTTAAAAGACTTAACAAAAATTGATAATTTAGTAAACGAATTAAATAATCTTGAAAATAAAATTAAATTAGATGCAGACAATTCTAAAATAGATAAATTAATAAGAAACGAATTAAAATCAATTGCTGATAATTTATCTTTAACTTTAAAGGCAATTAATCTAGAAGCATTATTGAATAATATTAATAGCAAAGATATTGCCAGTTATTTAAATAAATTGCAAGAAATTAAGCAAATAACCTCGCTTAAAATTCAAAAAACTAAATTTATTAATTTGGCATTGCAACAAAAATTCCAAGACCAAGTAATTGAAAATATTATCAATAAAATTGCAAAAAACAATTTAAAAGAAAAATATATATCATTAATAAATCAATATCAATATTTTAAGGGACAAATTATTGAAGAAATTAACAAATGA", "aa": "MRKKLLYILPLSSTILITPLVAASCYKKPKQPKQNIETTQTKDENAQKTEELLKKFNDAKTDLFSYLKDNFSTRETEQFNTLKEVQDAKKLLESFNKESLESSIESLNEKIQKINVAKQKILKIQPLLKDLTKIDNLVNELNNLENKIKLDADNSKIDKLIRNELKSIADNLSLTLKAINLEALLNNINSKDIASYLNKLQEIKQITSLKIQKTKFINLALQQKFQDQVIENIINKIAKNNLKEKYISLINQYQYFKGQIIEEINK", "aa_hexdigest": "cdfb2c45aabf02fee8205d5b5d3bb73e", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 333785, "stop": 334888, "observations": { "mutation": [ 334006, 334090 ], "stop": [ 334006, 334090 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9N2", "query_cov": 1.0, "subject_cov": 0.7247956403269755, "identity": 0.992, "score": 418.0, "evalue": 1.82e-144, "gene_start": 1, "gene_end": 266, "reference_start": 1, "reference_end": 266, "reference_sequence": "MRKKLLYILPLSSTILITPLVAASCYKKPKQPKQNIETTQTKDENAQKTEELLKKFNDAKTDLFSYLKDNFSTRETEQFNTLKEVQDAKKLLESFNKESLESSIESLNEKIQKINVAKQKILKIQPLLKDLTKIDNLVNELNNLENKIKLDADNSKIDKLIRNELKSIADNLSLTLKAINLEALLNNINSKDIASYLNKLQEIKQITSLKIQKTKFINLALQQKFQDQVIENIIDKIAKNNLKEKYIPLINQYQYFKGQIIEEINKWHANDNLTQSLNEQINSISQKLALNESIWEFVETLTDAEINSNKIFQKPDGQDFIEKFINPYINKLVNNEKDSSYTNFKQVEHDKREFNEIYHKKINSLIK" }, "paralog": false, "identity": 0.9754768392370572, "score": 629.0, "evalue": 2.67e-230, "description": "Internal stop codon at 334006, 334090. Nonsense mutation around 334006, 334090.", "psc": { "uniref90_id": "UniRef90_A0A454C9N2", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A454C9N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9N2", "UniRef:UniRef50_A0A454C9N2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9N2" ], "product": "Lipoprotein" } }, "id": "DFOKDJOJIG_608", "locus": "GCA013367855_00592" }, { "type": "cds", "sequence": "contig_1", "start": 335102, "stop": 335725, "strand": "-", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175972268.1", "SO:0001217", "UniParc:UPI00158CE3F4", "UniRef:UniRef100_UPI00158CE3F4", "UniRef:UniRef50_D1J831", "UniRef:UniRef90_D1J831" ], "nt": "ATGAGAAATAAAATTCGCTTATTAATACTAAGTGCCGTTGCAACTTCAGTTGTTGCAATGCCATTAGTTGCAGCAGGATGCAAAACAACAGATAATAGTCAAGATTCTACGACCACAGGTGAAGATTCGACCGGACAATCTCAATCTGGCGACATTGTAGATCCTAATAAGAGAGCAAAAGGTCAATTAGGCCAAAGCCAATTGTCATATATTAGAGACCAATTTGGTTTTACTTTAACTAATGAAGGTAAAGAAATATTAAATGACCCTGCACGTGGAACAAAAGAATTACAAAGAATTTTAAAAGAAATAATCAATAAGTATAAATCTATTGATGAACAAATTGAAATCAACATAAGAAAAAAACAAACTAGTGGAATAAATTCGCAAGTTTATAAAGCATATAAAAATGTAATAAATGATGCAGATTTTGTAAAATATTTTAAGGTTACATTGCCTAAGATACTTATTCCGTTTGGCCACCCCATTTCAATACAACTAAAATCAGAGGGAAGATTAAGAGATTTGCCAGTAATTTCTTATAGTGTAATATGCCCTGATAAATCAAATAATGGAATTTTTAGTAGTGATGGTGATAGCGAAGTACCACTTGAAATTGATTAA", "aa": "MRNKIRLLILSAVATSVVAMPLVAAGCKTTDNSQDSTTTGEDSTGQSQSGDIVDPNKRAKGQLGQSQLSYIRDQFGFTLTNEGKEILNDPARGTKELQRILKEIINKYKSIDEQIEINIRKKQTSGINSQVYKAYKNVINDADFVKYFKVTLPKILIPFGHPISIQLKSEGRLRDLPVISYSVICPDKSNNGIFSSDGDSEVPLEID", "aa_hexdigest": "7b60375c8d89b9e1a4abf826ba4b9256", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00158CE3F4", "ncbi_nrp_id": "WP_175972268.1", "uniref100_id": "UniRef100_UPI00158CE3F4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158CE3F4", "RefSeq:WP_175972268.1", "UniRef:UniRef100_UPI00158CE3F4" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158CE3F4", "uniref90_id": "UniRef90_D1J831" }, "psc": { "uniref90_id": "UniRef90_D1J831", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J831" }, "pscc": { "uniref50_id": "UniRef50_D1J831", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J831" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "DFOKDJOJIG_609", "locus": "GCA013367855_00593" }, { "type": "cds", "sequence": "contig_1", "start": 335727, "stop": 336275, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_036438525.1", "SO:0001217", "UniParc:UPI0005145C93", "UniRef:UniRef100_A0A454C9L3", "UniRef:UniRef50_D1J832", "UniRef:UniRef90_D1J832" ], "nt": "ATGAATAAAAAGATTACACTATTTTGTTTACCAATAGTTTTAATAATTCCAACAATTGCAGCAGTTTCATGCCAAAAATCCTCTAATTTAAAAACCAAAAATGATTTATTAAAAACCAAGATTATTGAAGGAATTAATTGCCCATGTCATACTAAATATAATGTTTTATCTAATAATAATCAAAACATGTCTAGTACTATGCTTGCAGCAATTTATGATAGTTTTAATTTTGAATCCAAAATACCGCTTAATCAAGAGAATAAGAAATTTTTATTCAAAATCATTGATAAACTCAATGATAAATACAAAAAAGATGATCAAAAGTCAAACATATTCAGTGATGAGTTATTTTTAAAATATTTTAAAATTTCATTGCCTGATTCGAAATACTTATTAAATCATACATTGAAATTAGAACTTGGATATGGTGATACAAATTTAAAATCAGATACTATATTTATAAAATATAGTATAATATGTAAATTAAGAAGTAACCAATTTTTACAAGTTATTGAAGATGAACGATATGTTTATATTCAGGGGGAATAA", "aa": "MNKKITLFCLPIVLIIPTIAAVSCQKSSNLKTKNDLLKTKIIEGINCPCHTKYNVLSNNNQNMSSTMLAAIYDSFNFESKIPLNQENKKFLFKIIDKLNDKYKKDDQKSNIFSDELFLKYFKISLPDSKYLLNHTLKLELGYGDTNLKSDTIFIKYSIICKLRSNQFLQVIEDERYVYIQGE", "aa_hexdigest": "aa3cfb5ad89fa03293c736da0b9596e4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0005145C93", "ncbi_nrp_id": "WP_036438525.1", "uniref100_id": "UniRef100_A0A454C9L3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005145C93", "RefSeq:WP_036438525.1", "UniRef:UniRef100_A0A454C9L3" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9L3", "uniref90_id": "UniRef90_D1J832" }, "psc": { "uniref90_id": "UniRef90_D1J832", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J832" }, "pscc": { "uniref50_id": "UniRef50_D1J832", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J832" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "DFOKDJOJIG_610", "locus": "GCA013367855_00594" }, { "type": "cds", "sequence": "contig_1", "start": 336275, "stop": 336778, "strand": "-", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_012855523.1", "SO:0001217", "UniParc:UPI0001BC21FB", "UniRef:UniRef100_D1J833", "UniRef:UniRef50_D1J833", "UniRef:UniRef90_D1J833" ], "nt": "ATGAAAAAAATAATATTATCTATAGGGGCAGTTCTAGTTTTAGCTTCGCCAATAATTTCAGCATCTTGTGTTTCGACTGATGAAAATACAGCAAAAGCAAATGAAATTTACACTAATAAAAGTGGAATTTTTAATTCTTCTCAATTAGAAGCAATTAAAAATGATTTTGTTTTCGAACTAACTGAACAGAGCAAAATACTAAAAAATAATTATGGCAATAAGGCGTTGGCAGATGAGCTAAAAAAGATTTGTAAAGATTATGAATTGCAAATTAATCTTAGCCCTTCTGAAAATAATAAATTAGCAGGTTTGAGGTTAATTAATAATGCAAACTTTTTAAAATTGTTTAAAGTGGTTAAGCCGAATTTAGGTGTAAATCACCAATTGATAATTAATTTTAAAATTGATAATAATAACAATATTTCATTAATCTATGACATATATTGCAAAGATGTAAAAACATATGATGCCAAAGATCAAGAAATAAAATTAGATTTAGAATAA", "aa": "MKKIILSIGAVLVLASPIISASCVSTDENTAKANEIYTNKSGIFNSSQLEAIKNDFVFELTEQSKILKNNYGNKALADELKKICKDYELQINLSPSENNKLAGLRLINNANFLKLFKVVKPNLGVNHQLIINFKIDNNNNISLIYDIYCKDVKTYDAKDQEIKLDLE", "aa_hexdigest": "c0e3d2396ac023e414bd2846a3e2afb3", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC21FB", "ncbi_nrp_id": "WP_012855523.1", "uniref100_id": "UniRef100_D1J833", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21FB", "RefSeq:WP_012855523.1", "UniRef:UniRef100_D1J833" ] }, "ips": { "uniref100_id": "UniRef100_D1J833", "uniref90_id": "UniRef90_D1J833" }, "psc": { "uniref90_id": "UniRef90_D1J833", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J833" }, "pscc": { "uniref50_id": "UniRef50_D1J833", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J833" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "DFOKDJOJIG_611", "locus": "GCA013367855_00595" }, { "type": "cds", "sequence": "contig_1", "start": 336788, "stop": 337636, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J834", "UniRef:UniRef90_D1J834" ], "nt": "ATGGTAAACATTTTTAAGCCATTGATTCCAAAAAGTGTTCATGAAAATAGTCTTGACTTTATTGACAATAGTTATTATCTTGCAAACAAAAAAATATTAAACGATCTTCAATCAAGCAATCTTGATTACAAATTAAAATCAGCTTATTTTCAAGAAATATCTAAACAACTAAATGATATAATAAAAAAGTTTTATGAACAACATCCAGATGCTGCTGATATTGAATTTGATATTCCACTAAATTTTTTGAATTTGCAATTTAATAACCAAAGGCAAAATGTTTTGGAACATATCAAAACAATATTTAAAGCAGTAAATAAACATTTAAAGCCAAATTTTGTAGTTATTGATGATTTAGCAAAATATGACTTGTATTTTAATCAAGATGCTTCAATATATTGCAATAATAGCTTTGAACTTTTAAAATCAAATACTAAAGAATTTATAGCAACAGCCATTGCTAAAAACTATAAATTGTTTTTAATCTTTAATAAAATTTCTAGCTTAACTAAAAGCAATAGTTTTATTCTAGAAAATACCAAAAAAATATTAAATGAATTAAATAAAACAAAACTAATTACAAATAATGAAATTTTTAACAAAAATTTAGAAGATTACAACAAAAATTTTTCTATAATAAAATCAATTATATTAAAATACTTAGAAAATATCTCTTACCAAGACCAATTAAATATAATTAATGATATTAACAGTTTACTTTCTTATACCTTTTCATTTGAAAATATCATTAAAACTGTTGGTTTTTCAAAAGTAATATACCAAAAATTTATTAATAAACCAATTAGTTATGACGGGTTGTCTTACTTGCAAGATATTGTAATAGAATAG", "aa": "MVNIFKPLIPKSVHENSLDFIDNSYYLANKKILNDLQSSNLDYKLKSAYFQEISKQLNDIIKKFYEQHPDAADIEFDIPLNFLNLQFNNQRQNVLEHIKTIFKAVNKHLKPNFVVIDDLAKYDLYFNQDASIYCNNSFELLKSNTKEFIATAIAKNYKLFLIFNKISSLTKSNSFILENTKKILNELNKTKLITNNEIFNKNLEDYNKNFSIIKSIILKYLENISYQDQLNIINDINSLLSYTFSFENIIKTVGFSKVIYQKFINKPISYDGLSYLQDIVIE", "aa_hexdigest": "559433d1b609302a229ee012680d079a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 336788, "stop": 337936, "observations": { "mutation": [ 337714, 337903 ], "stop": [ 337714, 337903 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J834", "query_cov": 1.0, "subject_cov": 0.6482758620689655, "identity": 0.993, "score": 540.0, "evalue": 3.23e-191, "gene_start": 1, "gene_end": 282, "reference_start": 154, "reference_end": 435, "reference_sequence": "MPISKNTFGMQMYHAFLQNIVTTLNFDDLDINQQQEILHKYNDFNISYTRNVSKNKLSNNITINWMPTANKKFFNDSFSMLAYGLKANQLNENTNINKILKSKNIAFLLLIHQLINQYGMLANQGDIWTSQAPSDLDISSFDSGINYHVLKDSMVNIFKPLIPKNVHENSLDFIDNSYYLANKKILNDLQSSNLDYKLKSAYFQEISKQLNDIIKKFYEQHPDAADIEFDIPLNFLNLQFNNQRQNVLEHIKTIFKAVNKHLKPNFVVIDDLAKYDLYFNQDASIYCNNSFELLKSNTKEFIATAIAKNYKLFLIFNKISSLTKSNSFILENTKKILNELNKTKLITNNEIFNKNLEDYNKNFFIIKSIILKYLENISYQDQLNIINDINSLLSYTFSFENIIKTVGFSKVIYQKFINKPISYDGLSYLQDIVIE" }, "paralog": false, "identity": 0.981675392670157, "score": 738.0, "evalue": 1.05e-271, "description": "Internal stop codon at 337714, 337903. Nonsense mutation around 337714, 337903.", "psc": { "uniref90_id": "UniRef90_D1J834", "uniref50_id": "UniRef50_D1J834", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J834", "UniRef:UniRef50_D1J834" ] }, "pscc": { "uniref50_id": "UniRef50_D1J834", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J834" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 33076.53910000005, "isoelectric_point": 8.286137580871586 }, "id": "DFOKDJOJIG_612", "locus": "GCA013367855_00596" }, { "type": "cds", "sequence": "contig_1", "start": 337712, "stop": 337900, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAACTGCAAATAAAAAATTTTTTAATGATAGTTTTTCAATGTTAGCATACGGTTTAAAAGTAAACCAATTAAATAAAAATACGAATATAAATGAAATATTAAAATCCAAAAATATTGCCTTTTTATTATTGATTCACCAATTGATTAATCAATATGGGATGTTGGCTAACCAAGGTGATATTTGA", "aa": "MPTANKKFFNDSFSMLAYGLKVNQLNKNTNINEILKSKNIAFLLLIHQLINQYGMLANQGDI", "aa_hexdigest": "9978bd0e744cfbf1d9b75e69ba277ed2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7058.164999999999, "isoelectric_point": 9.523801612854005 }, "id": "DFOKDJOJIG_613", "locus": "GCA013367855_00597" }, { "type": "cds", "sequence": "contig_1", "start": 337901, "stop": 338095, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATATCAAAAAATACTTTTGGTATGCAAATGTATCATGCATTCTTGCAAAATATTGTAACTACATTGAATTTTGACGATCTTGACATAAATCAACAACAAGAAATTTTGCATAAATATAATGATTTTAATATAAGCTATACTCGTAATGTTTCTAAAAATAAGTTGAGTAATAATATTACCATTAATTGA", "aa": "MPISKNTFGMQMYHAFLQNIVTTLNFDDLDINQQQEILHKYNDFNISYTRNVSKNKLSNNITIN", "aa_hexdigest": "3364ab9bd125409e701de2e7bc69a378", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7553.4361, "isoelectric_point": 6.698270606994629 }, "id": "DFOKDJOJIG_614", "locus": "GCA013367855_00598" }, { "type": "cds", "sequence": "contig_1", "start": 338249, "stop": 338473, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATTTTGATTTATTTGAATATTTAAAAAGTTACAACGTTGATGTTTATAAATTACTAGACTTTAACGACAAAAATTTAAGTATTTGTAGTGCTGATAATATTGAACATGATTGGGTTGATTTATTTAACAATTTGTTTATACATTCAAACTACATTGATGCAATGCCTTATGAAATCATTAATGAAAAATATAAAGATTTTTTAACAAACCTTGACTGA", "aa": "MNNFDLFEYLKSYNVDVYKLLDFNDKNLSICSADNIEHDWVDLFNNLFIHSNYIDAMPYEIINEKYKDFLTNLD", "aa_hexdigest": "54e815ca0da7a361133d60ab1400fbe5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8952.881899999993, "isoelectric_point": 4.243792533874512 }, "id": "DFOKDJOJIG_615", "locus": "GCA013367855_00599" }, { "type": "cds", "sequence": "contig_1", "start": 338654, "stop": 339217, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAACATTCTTATTTTAAGTTCAATGTTAGCATTATTTACAATGACATCTGCGATATCAATATCTTGTAAATTTAATAATTTTAATGAAGACAAGAAAAAATATATTCAAAAGCTTAATATTGCAAATCCTTATTTAGAATATGGCAGTTCAATACCAAATTTTAAAAACCAAAATTTAAAAGAAATAAATTTAATGACAGGAGCCAAAGTTCTTAGAATTTATTCAAAAAATCAACCTAAAATAGACTTTAGGGATAATATAGTTTTGCGCCCGACTGAATTAAGTTATAAATTTGAATATGGTAATAGTATTCAAATAAATGATAAACTTTTCAATAGCGATGATAAACTAAATGTTGACTATCAAAACACTGAATTGAATAAGCAAGATCCCAACAAATATTACCCCAGCAAAGACAAGGGCAATGGTTTTAATAATCCATATTTGTTCGTTCCTTCAAAAAATTCAATGTCTATAAATAGCAAACAATTCATTGAATCTCTAAGAAAAGCAAAAAATATTTCAATCAGTATAAATCAAAGAAAAAATTATTGA", "aa": "MKKNILILSSMLALFTMTSAISISCKFNNFNEDKKKYIQKLNIANPYLEYGSSIPNFKNQNLKEINLMTGAKVLRIYSKNQPKIDFRDNIVLRPTELSYKFEYGNSIQINDKLFNSDDKLNVDYQNTELNKQDPNKYYPSKDKGNGFNNPYLFVPSKNSMSINSKQFIESLRKAKNISISINQRKNY", "aa_hexdigest": "087f6aa4bd71659b426c1c56cf903b00", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21767.671700000003, "isoelectric_point": 9.670080375671386 }, "id": "DFOKDJOJIG_616", "locus": "GCA013367855_00600" }, { "type": "cds", "sequence": "contig_1", "start": 339228, "stop": 339407, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCATTAGGCGGAGGAATTATTACATTTATGTCCATATTTGAATTGGTTCCAGAATTTATCCATGTTAGAAATCAATCTCCGAAAGTTTTATATACTTCATTTGCTTTATTTAGTTTAGGACTAGTATTAACTATAGTTATTTTATGTTTTCACACTCATACAGCAACGGCATTTTAA", "aa": "MALGGGIITFMSIFELVPEFIHVRNQSPKVLYTSFALFSLGLVLTIVILCFHTHTATAF", "aa_hexdigest": "df9eba0a2f797faf690ad37dba9b4957", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6514.716899999998, "isoelectric_point": 6.806889915466309 }, "id": "DFOKDJOJIG_617", "locus": "GCA013367855_00601" }, { "type": "cds", "sequence": "contig_1", "start": 339423, "stop": 339704, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATGAGTCATAGAATTATTGATGGACTATTTTTGGGATATAATGTTTATACATTAACATCAATTGGTGGAACATATAAAGCAAATATTCCTTTAATAATAACATTTAATATTCATATATTACTTGAAATTTTAGTTGTTTATTATCGTCAAATTCAATATGGAGAAAAGAAATCAAAAGCCATACTATACAACTTCTTAACATTTTTATTAATTATCCCATTTATGTTAATTGGCGGATTGCTTGGCCAATATATTGAACATAGTAAAGTGTTTTGA", "aa": "MLMSHRIIDGLFLGYNVYTLTSIGGTYKANIPLIITFNIHILLEILVVYYRQIQYGEKKSKAILYNFLTFLLIIPFMLIGGLLGQYIEHSKVF", "aa_hexdigest": "6c261c62e31d345c939dfab4a8d16dc6", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10745.78719999999, "isoelectric_point": 9.260512733459471 }, "id": "DFOKDJOJIG_618", "locus": "GCA013367855_00602" }, { "type": "cds", "sequence": "contig_1", "start": 339945, "stop": 340172, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATAGGAACAAGTGCATTTAACGGAAATATAACTTTAGCAACATTTGTAAATCTAATAATCTATATTTTAATATTATTGGGCGTACCTTCATTAGTTTTGGGCGTAATTTCTATATTTAAATTAAAAATTACTGATAAGAAAAATATTTATATTTATGCATTTGCTACGGGAATGTTTCTAATGATTGGCACTGTAGGTTTCTTTAAAGAAAGTTTTGATTGA", "aa": "MFIGTSAFNGNITLATFVNLIIYILILLGVPSLVLGVISIFKLKITDKKNIYIYAFATGMFLMIGTVGFFKESFD", "aa_hexdigest": "2dc2ffb6a1c8af9c24258720723a8558", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8289.930899999998, "isoelectric_point": 9.158330345153807 }, "id": "DFOKDJOJIG_619", "locus": "GCA013367855_00603" }, { "type": "cds", "sequence": "contig_1", "start": 340253, "stop": 340720, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAAGTTTTATTTTAATTCTATTTTAATTGCTAGCGCACTTAGCTTTATGCCTTTAATAATTTGTAGTTGTCAAACTAAAAAAGATAACTATATTATAGAGGGTAGCAATGCTTATATAGATATTTCAAAAATTTCTAGAATTTTTTTGCATAGGTTAAGCATAGGCCAAATTGCTGCACTACACAACATGTATAAGATTTTTTATTACTATGATAATTCTAATAAAAAGCAATATTATGACTCTGCATATGTAAATGGCCAAAAGCTATATTTAAGAAATGCAAATAAAAATGTTGAATATAAATTAAATTTTCCAATTAAACCTTCTTGGAAACAAGAAATATCAAAATTTGACAATATTAATATTGTAAAAAGCAATAAACCAAGTGATATTAATAATTTTTTAAACCAATATAGTTTTGATGAAGTAGATTCTGCAGGCGATGTTAATGACGAATGA", "aa": "MKKKFYFNSILIASALSFMPLIICSCQTKKDNYIIEGSNAYIDISKISRIFLHRLSIGQIAALHNMYKIFYYYDNSNKKQYYDSAYVNGQKLYLRNANKNVEYKLNFPIKPSWKQEISKFDNINIVKSNKPSDINNFLNQYSFDEVDSAGDVNDE", "aa_hexdigest": "1703ab8a2d902ccc34275656ed366330", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18134.44329999999, "isoelectric_point": 9.039644050598145 }, "id": "DFOKDJOJIG_620", "locus": "GCA013367855_00604" }, { "type": "cds", "sequence": "contig_1", "start": 340787, "stop": 340930, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACTGTTATTTTTAGATTAATTCAAGATACAGAATTAAATTATTCAATAATGAAATCTAAATATTTAATAAATTCTAAAAGAGAAAATGTAATTACACAAAATTTATTTAAAAACAAATACATTCAAGCTAGTTCATGA", "aa": "MQTVIFRLIQDTELNYSIMKSKYLINSKRENVITQNLFKNKYIQASS", "aa_hexdigest": "4154c14e804a354ed385c6b44658f08f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5613.466700000002, "isoelectric_point": 9.775808525085449 }, "id": "DFOKDJOJIG_621", "locus": "GCA013367855_00605" }, { "type": "cds", "sequence": "contig_1", "start": 341366, "stop": 341788, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTTTTTCAAAGCTTTAAAAATGAACCTGGTTTCTTTAAATTAGCAGTTCCCGAATATAAAAAAAATGAAGATAAGGAATATAAAATTATTGATTTTGAATATACTCCATATTTTGAAACAAATCAAATTTTTAAAGCAATAGTAAGGGTATTTAAATTAAATGGTACATATAAAGATTATGTTTTAATATCTTCTAATTTTGATGATCACGGTCACAGATTAAAAGGCTTAATTACTAAAAATGCTTTACCAAATGAACTTAAAGCTTCTGATATATATTCAATTAGAGCAGATAGTGAGCCAATTAAACAAGGAATCAAATTGGATGATTTCATTGACAAGAATAATAAAAATAGTGTTTTTAGATATATGTTAAATGAATCTGCAAAAAAACTAGAAAATATTTTTGAATATTGA", "aa": "MYLFQSFKNEPGFFKLAVPEYKKNEDKEYKIIDFEYTPYFETNQIFKAIVRVFKLNGTYKDYVLISSNFDDHGHRLKGLITKNALPNELKASDIYSIRADSEPIKQGIKLDDFIDKNNKNSVFRYMLNESAKKLENIFEY", "aa_hexdigest": "8f3dd4711e0b6692f6d7efcae230fe14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16605.708199999986, "isoelectric_point": 7.796758079528809 }, "id": "DFOKDJOJIG_622", "locus": "GCA013367855_00606" }, { "type": "cds", "sequence": "contig_1", "start": 341828, "stop": 342085, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGCCAAAGATCCATTCCAATTAAAATTGTTAGCTTCTTATTTAAATAACTACTTATTAGCATATGCACTTGAAAATAAGGCAGGTGAAGTATTTAGTGGAATTAAACGCATCGATCTTAACATAGTTAAATTGCCAAATCAACCAATTGGAAGATTATATTTAAAAATGGATTTTGTTTCATATGCCAATAACCAAGATTTCAAATTCAAATCAAAAAATGAAAAAATTGTAAAAAGTGTTTATTTATATTGA", "aa": "MLAKDPFQLKLLASYLNNYLLAYALENKAGEVFSGIKRIDLNIVKLPNQPIGRLYLKMDFVSYANNQDFKFKSKNEKIVKSVYLY", "aa_hexdigest": "90a334c80318eee6e758a6d23630ff18", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9882.500599999992, "isoelectric_point": 9.58440189361572 }, "id": "DFOKDJOJIG_623", "locus": "GCA013367855_00607" }, { "type": "cds", "sequence": "contig_1", "start": 342169, "stop": 342546, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATCAATAAAATTGCTATCCTTACTTTGTACTTCTACAATAATTTCATTGCCTGCTATTTCTTGCACTATCAAAGTAAATCACGATAACGAAGACAAGGAAAATTTAGATTTTTCAAGTGATTTTAGTGGCAAAAAGCAAAAAAATGAATTCAATTTCAAAAATAAGATTTCTCAAAATATTTATGATTTGTATCAAAATAAAATTGCTATTTTATTTAACGATGTAAAAACTAATTACCGGGGATATAAAAAAATATTTGGATCTTTAACAAGAAACTTAAACACTCTAAGGGCTAAGATTAATAGCTTAAGATACGAACAAAGTTTAAAAGAAAACAAAAAAATATTAAATGATTTTTACAATAATTGA", "aa": "MKKSIKLLSLLCTSTIISLPAISCTIKVNHDNEDKENLDFSSDFSGKKQKNEFNFKNKISQNIYDLYQNKIAILFNDVKTNYRGYKKIFGSLTRNLNTLRAKINSLRYEQSLKENKKILNDFYNN", "aa_hexdigest": "44f8f9321ebd60c40bb3a5603820618c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14610.592599999982, "isoelectric_point": 9.645195579528806 }, "id": "DFOKDJOJIG_624", "locus": "GCA013367855_00608" }, { "type": "cds", "sequence": "contig_1", "start": 342565, "stop": 342762, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAAAATAATCCATTTGCATTATATTTATTCAAATATAGTTTAATTTTTTCTGATGTGGATGCTGTATTAGCTGATACAAATCTTGTTTTTGAAACTTCTGAATTTTTAAAATATTTAAAAACAATTGATGACAGACTTGAAGGTAAAGACATTGATTTAGGTCAAACTCAACATGCATTACAAGCGGTTTGA", "aa": "MLKNNPFALYLFKYSLIFSDVDAVLADTNLVFETSEFLKYLKTIDDRLEGKDIDLGQTQHALQAV", "aa_hexdigest": "53ee0f7517b2a8fe14d8b8609555d331", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7441.420799999998, "isoelectric_point": 4.562545585632326 }, "id": "DFOKDJOJIG_625", "locus": "GCA013367855_00609" }, { "type": "cds", "sequence": "contig_1", "start": 342814, "stop": 343053, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATAAAATTAACATTGAAGATGATAAAAACTCGCATAATCACTCACATGCAATCATAAATTTAACATATGAAATGGGTTTATGGCATGAAATGCTGGCAAAAAATAAACTTCAAAATTACGAAGAATTTCAAGAAGAATATAAAATTGCTAAAAAACACATAATCGATAATATAAACAATATTGATGTAAATAATAATTATTTAGCATTAAAAAAAGTCCTAGATAAAGATTGA", "aa": "MLDKINIEDDKNSHNHSHAIINLTYEMGLWHEMLAKNKLQNYEEFQEEYKIAKKHIIDNINNIDVNNNYLALKKVLDKD", "aa_hexdigest": "137ef8e2b8887a877149fa641915a194", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9438.56129999999, "isoelectric_point": 5.655900382995605 }, "id": "DFOKDJOJIG_626", "locus": "GCA013367855_00610" }, { "type": "cds", "sequence": "contig_1", "start": 343587, "stop": 343970, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAGAAATTAATTTCTTAAGAATGGGATTTGCAATGATTTTTGGTACAGAAGATATGTATAGGAAAACTATAAAAGGTATGTATCGAGACGCATTTAGTTGCGAAGCTGAAATTGTAGAAATTGATTACAAAAGTTCATTGCCAATTGGTGAATGTGGAATTGATGCGTTTGGAAATAAGCCTTATTTTGAAGACATTGGAAATATTAAAAGAATAATTTGTGCAATCGGCAAATTAGACACGATGTTTGATGCGATGATACCTTATGATAAAGATTTAAAAATATTAGGTGGATCTTCAGACCATTTAATAATTAATGCTAACGATTCTAAAATTAATTATAAATGTGGGGACATACTTAAATTTGATTTAGATTGA", "aa": "MPKEINFLRMGFAMIFGTEDMYRKTIKGMYRDAFSCEAEIVEIDYKSSLPIGECGIDAFGNKPYFEDIGNIKRIICAIGKLDTMFDAMIPYDKDLKILGGSSDHLIINANDSKINYKCGDILKFDLD", "aa_hexdigest": "38f888a92d80150d9ecec61b56269e7a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14385.549899999978, "isoelectric_point": 4.871636009216308 }, "id": "DFOKDJOJIG_627", "locus": "GCA013367855_00611" }, { "type": "cds", "sequence": "contig_1", "start": 343980, "stop": 344600, "strand": "-", "frame": 2, "gene": null, "product": "Alanine racemase", "db_xrefs": [ "EC:5.1.1.12", "KEGG:K21898", "SO:0001217", "UniRef:UniRef50_A0A077L6Y5", "UniRef:UniRef90_A0A448ZWG6" ], "nt": "ATGTCATATCCCAAAATAATTATTGATGAGAAAAAATTTAAAAATAATATAAAAGTTGCTATTGAAATGTGTAAAAAACACAATATAGAAGTTCTTGCCGTTACTAAAGGTTTTTGTGGAAATCAAAGAATGGCTGAACTATATTATGAAGCAGGAATTAGGTATTTTGGTGATTCTAGACTTGAAAACTTTACTATCTTTAATAAATTTAAAGATGTTAAAAAACAATTATTAAGAATACCAATGATTTCAGAAATTCCTGAAGTTGTAAAAATTTGTGATTCAAGTTTAAATGGGGATATTCAAGTAATAGAAAAAATTAGTGAATATTGTCTTAAAAATAATTTAAAACCACATGAAATAGTTTTAATGATAGATTTAGGCGATAGAAGAGAAGGTGTTTTACCTGAAGATGCTTTGAGCACTGCTAAAAAAATACATGAACTAAAAGGTGTTAAATTAATTGGGTTAGGCTGTAACTTCGGTTGCTATGGCGCTAGAGTTCCCTCAGATGAATCAATGAAATTATTTGTAAATATTCAACACAAAATTGAAGATGAACTAAAAATTAAATTAACTCATATGTCTGGCGGAAATTCACTAAGCTTGCACCTAGTTTGA", "aa": "MSYPKIIIDEKKFKNNIKVAIEMCKKHNIEVLAVTKGFCGNQRMAELYYEAGIRYFGDSRLENFTIFNKFKDVKKQLLRIPMISEIPEVVKICDSSLNGDIQVIEKISEYCLKNNLKPHEIVLMIDLGDRREGVLPEDALSTAKKIHELKGVKLIGLGCNFGCYGARVPSDESMKLFVNIQHKIEDELKIKLTHMSGGNSLSLHLV", "aa_hexdigest": "a0c5a7431b7a59db6a590b909c508ef5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 343677, "stop": 344600, "observations": { "mutation": [ 343982 ], "stop": [ 343982 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A448ZWG6", "query_cov": 1.0, "subject_cov": 0.5786516853932584, "identity": 1.0, "score": 412.0, "evalue": 1.51e-143, "gene_start": 1, "gene_end": 206, "reference_start": 1, "reference_end": 206, "reference_sequence": "MSYPKIIIDEKKFKNNIKVAIEMCKKHNIEVLAVTKGFCGNQRMAELYYEAGIRYFGDSRLENFTIFNKFKDVKKQLLRIPMISEIPEVVKICDSSLNGDIQVIEKISEYCLKNNLKPHEIVLMIDLGDRREGVLPEDALSTAKKIHELKGVKLIGLGCNFGCYGARVPSDESMKLFVNIQHKIEDELKIKLTHMSGGNSLSLHLVWENRMPKEINFLRMGFAMIFGTEDMYRKTIKGMYRDAFSCEAEIVEIDYKSSLPIGECGIDAFGNKPYFEDIGNIKRIICAIGKLDTMFDAMIPYDKDLKILGGSSDHLIINANDSKINYKCGDILKFDLDWGSLLYLFNSKYVKKEFKK" }, "paralog": false, "identity": 0.996742671009772, "score": 617.0, "evalue": 1.29e-226, "description": "Internal stop codon at 343982. Nonsense mutation around 343982.", "psc": { "uniref90_id": "UniRef90_A0A448ZWG6", "product": "Alanine racemase", "ec_ids": [ "5.1.1.12" ], "uniref50_id": "UniRef50_A0A077L6Y5", "kegg_orthology_id": "K21898", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A448ZWG6", "EC:5.1.1.12", "UniRef:UniRef50_A0A077L6Y5", "KEGG:K21898" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L6Y5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L6Y5" ], "product": "Alanine racemase-like protein" } }, "id": "DFOKDJOJIG_628", "locus": "GCA013367855_00612" }, { "type": "cds", "sequence": "contig_1", "start": 345076, "stop": 345237, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0012AB1D60", "UniRef:UniRef90_UPI001F515A69" ], "nt": "ATGTCAAGAGAACAATTTGAATATCTCATTACTGATATTTTTTATGAAGAAATTTTAAAACGCAACAATAGCAAAAATTTAGAAGAAGTTGTTGAAAAAATTAAAAAGAATCATAATTTTTCAATATCAATAAAGCAAATTATCACTATTTTAGGGATTTAA", "aa": "MSREQFEYLITDIFYEEILKRNNSKNLEEVVEKIKKNHNFSISIKQIITILGI", "aa_hexdigest": "c211244167a109bababb55bd23a50b4f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 345076, "stop": 345375, "observations": { "mutation": [ 345235 ], "stop": [ 345235 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F515A69", "query_cov": 1.0, "subject_cov": 0.5353535353535354, "identity": 0.981, "score": 98.2, "evalue": 1.37e-25, "gene_start": 1, "gene_end": 53, "reference_start": 1, "reference_end": 53, "reference_sequence": "MSREQLEYLITDIFYEEILKRNNSKNLEEVVEKIKKNHNFSISIKQIITILGIKKSTFYYRIKKFKLPAKNKTIQYEEQIKEAFIECKGRFGRERLSLF" }, "paralog": false, "identity": 0.9494949494949495, "score": 185.0, "evalue": 2.11e-62, "description": "Internal stop codon at 345235. Nonsense mutation around 345235.", "psc": { "uniref90_id": "UniRef90_UPI001F515A69", "uniref50_id": "UniRef50_UPI0012AB1D60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F515A69", "UniRef:UniRef50_UPI0012AB1D60" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0012AB1D60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0012AB1D60" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6371.317099999998, "isoelectric_point": 6.556855201721191 }, "id": "DFOKDJOJIG_629", "locus": "GCA013367855_00613" }, { "type": "cds", "sequence": "contig_1", "start": 345423, "stop": 345953, "strand": "+", "frame": 3, "gene": "insO", "product": "Integrase-recombinase protein", "db_xrefs": [ "COG:COG2801", "COG:X", "SO:0001217", "UniRef:UniRef50_C4XED4", "UniRef:UniRef90_Q9PQB9" ], "nt": "ATGAATAAATTGGGTTTGTTATGTAAAATAAGAAGAGATAAAAGAAAAAGAGAAATAAAGAATGTTAATGCAACATTCAAAAACTTAGCCAATAGAGATTATGATGGCCAAATGAATGATATATATGCTACTGATGTGACATATATTCCTTCACCCACTGATGTTATAGAAAATCATGTATATTTATCGGTTTTATTGCATCATAAAACAAAGAAAATTGTGAGCTGGAATTTATCAAAATATAATGATACTAATCTTGTTTTAAAACATTTTAAACTAACTAAATTTCCTAAAAAATTCATTGTACATTCAGACTACGGATATCAATATTCCTCTAAGGAATTCCTTGATTACATAGATTCAATTAATGGCACTATTTCTATGTCAAGAGTTGGAAATTCTTTGGATAATAGAGAAGCAGAATATTTCTTTTCTATTCTAAAAACTGAAATATTTTCAAATTTTTTCAAAAAAGCAAAAAGTTTAACATTTAATGAATTGCAAACAGAAATAGACAAATTTATTAATTGA", "aa": "MNKLGLLCKIRRDKRKREIKNVNATFKNLANRDYDGQMNDIYATDVTYIPSPTDVIENHVYLSVLLHHKTKKIVSWNLSKYNDTNLVLKHFKLTKFPKKFIVHSDYGYQYSSKEFLDYIDSINGTISMSRVGNSLDNREAEYFFSILKTEIFSNFFKKAKSLTFNELQTEIDKFIN", "aa_hexdigest": "8146830f7dd4bd965e4c6c0c46f8e2c8", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_Q9PQB9", "query_cov": 1.0, "subject_cov": 0.8502415458937198, "identity": 0.972, "score": 340.0, "evalue": 7.25e-118, "valid": true, "gene": "InsO", "product": "Integrase-recombinase protein", "uniref50_id": "UniRef50_C4XED4", "cog_id": "COG2801", "cog_category": "X" }, "pscc": { "uniref50_id": "UniRef50_C4XED4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED4" ], "product": "Integrase catalytic domain-containing protein" }, "genes": [ "insO" ], "id": "DFOKDJOJIG_630", "locus": "GCA013367855_00614" }, { "type": "cds", "sequence": "contig_1", "start": 346257, "stop": 346949, "strand": "-", "frame": 2, "gene": "ddaH", "product": "Dimethylargininase (N[G],N[G]-dimethylarginine dimethylaminohydrolase)", "db_xrefs": [ "COG:COG1834", "COG:E", "EC:3.5.3.18", "KEGG:K01482", "SO:0001217", "UniRef:UniRef50_D5E539", "UniRef:UniRef90_A0A448ZVQ2" ], "nt": "ATGTGTAATTGTAAAAAATGTAATGATTGCAAAGATTGCTGCAAATCATGTGAAAAACCTTTAAAAAATAAATTTAATAGTATTATTGTTAAAACACCATGTGCTGCTATGGTAAATGGTATTTCATCAGCTGGCTTACATCCAGATTATGAACTTGCTAAAAAACAACACAAAGATTATATTGAAAAATTAAAGACTTGTGGTGTTGATATAACCGTTTTACCTTTAAATGAAAAATATCCAGACTCATGCTTCGTAGAAGATACTGCTGTTATTATTCCAGGTGAACTAGCTATTTTAACTAATCCAGGTGCTAAAACACGTAATGGAGAAAAAGTTGAAATGCTTCCTGCATTAAAGAAATACTTTGATGATGGACACATTGCAAAAATTGAAGCTCCCGGAACTGTTGACGGGGGAGATGTTATGATGGTTGGAGATACATACTACATTGGTATGTCCGAAAGAACTAATCGTGACGGAATTAGACAATTCCACAATATATTAGCAAAAATTGGTAAGAAGTTAGTTCCAGTACCAATGACAGAAATGTTGCACCTTAAAACAGGAGTTAACTATCTTGAACATAATAACTTATTAATTGCAGGTGAATTTGTTAACTATCCTACATTTGATTCATTTAACAAAATTGTAGTTCCTGCCGATGAAGCATATGCCGCAAACTGTATTTGA", "aa": "MCNCKKCNDCKDCCKSCEKPLKNKFNSIIVKTPCAAMVNGISSAGLHPDYELAKKQHKDYIEKLKTCGVDITVLPLNEKYPDSCFVEDTAVIIPGELAILTNPGAKTRNGEKVEMLPALKKYFDDGHIAKIEAPGTVDGGDVMMVGDTYYIGMSERTNRDGIRQFHNILAKIGKKLVPVPMTEMLHLKTGVNYLEHNNLLIAGEFVNYPTFDSFNKIVVPADEAYAANCI", "aa_hexdigest": "29ba2f2ae4da306d3d8a48eab33b41ca", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A448ZVQ2", "query_cov": 1.0130434782608695, "subject_cov": 0.8090277777777778, "identity": 0.9740000000000001, "score": 441.0, "evalue": 1.96e-155, "valid": true, "gene": "DdaH", "product": "Dimethylargininase (N[G],N[G]-dimethylarginine dimethylaminohydrolase)", "ec_ids": [ "3.5.3.18" ], "uniref50_id": "UniRef50_D5E539", "cog_id": "COG1834", "cog_category": "E", "kegg_orthology_id": "K01482" }, "pscc": { "uniref50_id": "UniRef50_D5E539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E539" ], "product": "Dimethylargininase (N[G],N[G]-dimethylarginine dimethylaminohydrolase)" }, "genes": [ "ddaH" ], "id": "DFOKDJOJIG_631", "locus": "GCA013367855_00615" }, { "type": "cds", "sequence": "contig_1", "start": 347327, "stop": 347548, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_036432935.1", "SO:0001217", "UniParc:UPI00051470FD", "UniRef:UniRef100_UPI00051470FD", "UniRef:UniRef50_UPI00051470FD", "UniRef:UniRef90_UPI00051470FD" ], "nt": "TTGAAAATTAATGACAGCAAAATTAGCAATAGTGAAAAATTAATTTATAAGGCGAATATTTTATTGATTGAAAACACTAAAAAATTGTCAATATATTCAAATAAAAACCAAAATTATAAATTTGGTGAAATTATTGAAGTAGATGCTTGCCAAGATAGATTTTTCGGAACCAATGAAAAATTCATATTTATTATGCAATTGATGCCAAAAGTGGTGCATTAG", "aa": "MKINDSKISNSEKLIYKANILLIENTKKLSIYSNKNQNYKFGEIIEVDACQDRFFGTNEKFIFIMQLMPKVVH", "aa_hexdigest": "35e5a4445d2976430f686de96dd5e6e2", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00051470FD", "ncbi_nrp_id": "WP_036432935.1", "uniref100_id": "UniRef100_UPI00051470FD", "db_xrefs": [ "SO:0001217", "UniParc:UPI00051470FD", "RefSeq:WP_036432935.1", "UniRef:UniRef100_UPI00051470FD" ] }, "ips": { "uniref100_id": "UniRef100_UPI00051470FD", "uniref90_id": "UniRef90_UPI00051470FD" }, "psc": { "uniref90_id": "UniRef90_UPI00051470FD", "uniref50_id": "UniRef50_UPI00051470FD" }, "pscc": { "uniref50_id": "UniRef50_UPI00051470FD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00051470FD" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8588.948300000002, "isoelectric_point": 9.09901943206787 }, "id": "DFOKDJOJIG_632", "locus": "GCA013367855_00616" }, { "type": "cds", "sequence": "contig_1", "start": 347620, "stop": 347994, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J842", "UniRef:UniRef90_UPI0003657047" ], "nt": "ATGGCAAAAATTAATGGTTCAATTATTTCAAAATATGAAATTAAAAAGATTAAAAATTTAGATTTTATTTCCAAAAATTTGCAAGAAAAAACAATGTTATCAATTCCTACAATTTGTAGATATAAAAAAATTATTAAAGATAATATGCAAGGTAGCAAAATTAAAGTAAATATTAGACATAAAAACTTTAATAATCAAAATGCAAAAATAATAGAAAACGGTTTTGCAAAAGACTTATTTTTGAAATATCAAATTGAAAATCAAAAAATAAATTCAAATGTTACTAAAAAATTTATTACTGTTAAAGATTTTTATGAAGACCTTAGTGAAGAAATAAAGTCAAAAATTTCGTTATCGTATTTATATAATGCTTGA", "aa": "MAKINGSIISKYEIKKIKNLDFISKNLQEKTMLSIPTICRYKKIIKDNMQGSKIKVNIRHKNFNNQNAKIIENGFAKDLFLKYQIENQKINSNVTKKFITVKDFYEDLSEEIKSKISLSYLYNA", "aa_hexdigest": "38d66170f5f0fc6a1a24e12489da201a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 347407, "stop": 347994, "observations": { "insertion": [ 347469 ], "deletion": [ 347553 ], "mutation": [ 347622 ], "stop": [ 347622 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0003657047", "query_cov": 1.0, "subject_cov": 0.6391752577319587, "identity": 0.976, "score": 231.0, "evalue": 1.03e-75, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MAKINGSIISKYKIKKIKNLDFISKNLQEKTMLSIPTICRYKKIIKDNMQGSKIKVNIKHKNFNNQNAKIIENDFAKDLFLKYQIENQKINSNVTKKFITVKDFYEDLSEEIKSKISLSYLYNAWLKHGFCSPYATHKIKRIAKNISNLKINDSKISNSEKLIYKANILLIENTKNCQYIQIKTKIINLVKLLK" }, "paralog": false, "identity": 0.9743589743589743, "score": 356.0, "evalue": 1.69e-127, "description": "Internal stop codon at 347622. Frameshift due to insertion around 347469. Frameshift due to deletion around 347553. Nonsense mutation around 347622.", "psc": { "uniref90_id": "UniRef90_UPI0003657047", "uniref50_id": "UniRef50_D1J842", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0003657047", "UniRef:UniRef50_D1J842" ] }, "pscc": { "uniref50_id": "UniRef50_D1J842", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J842" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14543.86439999998, "isoelectric_point": 9.754211616516113 }, "id": "DFOKDJOJIG_633", "locus": "GCA013367855_00617" }, { "type": "cds", "sequence": "contig_1", "start": 348321, "stop": 348941, "strand": "+", "frame": 3, "gene": null, "product": "DDE-type integrase/transposase/recombinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED4", "UniRef:UniRef90_UPI00158B17C2" ], "nt": "ATGCAAAGGAAGATTTGGAAGGGAAAGACTTTCACTTTTTTAAAGAAAAAGTACCAAATCGATATAAATCCAAGGACTCTAGGCAGAATTATGAATAAATTGGGTTTGCTATGTAAAATAAGAAGAAGCAAAAGAAAAAGAGAAATAAAGAATGTTAATACAACATTCAAAAACTTAGCCAATAGAGATTATGATGGCCAAATGAATGATATATATGCTACTGATGTGACATATATTCCTTCACCCACTGATGTTATAGAAAATCATGTATATTTATCGGTTTTATTGCATCATAAAACAAAGAAAATTGTGAGCTGGAATTTATCAAAATATAATGATACTAATCTTGTTTTAAAACATTTTAAACTAACTAAATTTCTTAAAAAATTCATTGTACATTCAGACCACGGATATCAATATTCCTCTAAGGAATTCCTTGATTACATAGATTCAATTAATGGCACTATTTCTATGTCAAGAGTTGGAAATTCTTTGGATAATAGAGAAGTAGAATATTTCTTTTCTATTCTAAAAACTGAAATATTTTCAAATTTCTTTCAAAAAGCAAAAAGTTTAACATTTAATGAATTACAAACAGAAATAGACAAATTTATTAATTGA", "aa": "MQRKIWKGKTFTFLKKKYQIDINPRTLGRIMNKLGLLCKIRRSKRKREIKNVNTTFKNLANRDYDGQMNDIYATDVTYIPSPTDVIENHVYLSVLLHHKTKKIVSWNLSKYNDTNLVLKHFKLTKFLKKFIVHSDHGYQYSSKEFLDYIDSINGTISMSRVGNSLDNREVEYFFSILKTEIFSNFFQKAKSLTFNELQTEIDKFIN", "aa_hexdigest": "23c1be6a1d70874d3cc04be2caff3dc0", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI00158B17C2", "query_cov": 0.8543689320388349, "subject_cov": 0.8543689320388349, "identity": 1.0, "score": 348.0, "evalue": 1.48e-120, "valid": true, "product": "DDE-type integrase/transposase/recombinase", "uniref50_id": "UniRef50_C4XED4" }, "pscc": { "uniref50_id": "UniRef50_C4XED4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED4" ], "product": "Integrase catalytic domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_634", "locus": "GCA013367855_00618" }, { "type": "cds", "sequence": "contig_1", "start": 349255, "stop": 349629, "strand": "+", "frame": 1, "gene": null, "product": "Helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "nt": "ATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGTTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCGAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAGTTTTCCAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGA", "aa": "MNYNIHKYFHLSLEERFFIQRSLENNLSLRAISRQLNRSASTISREIKRNSNFNEYIFLRADKKSKFRAYHKYMFKFNINFEFEEFSKIFKSKYDKKYFGVKATMNYIEENFNVKKPTMRTVFN", "aa_hexdigest": "c57bd1123260a439ee4f69e485682c4a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0AAJ1UWR4", "query_cov": 1.0080645161290323, "subject_cov": 0.8116883116883117, "identity": 0.6, "score": 141.0, "evalue": 1.13e-40, "valid": false, "product": "Helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_A0AAJ1UWR4" }, "pscc": { "uniref50_id": "UniRef50_A0AAJ1UWR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "product": "Helix-turn-helix domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_635", "locus": "GCA013367855_00619" }, { "type": "cds", "sequence": "contig_1", "start": 349813, "stop": 350094, "strand": "+", "frame": 1, "gene": null, "product": "IS30 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "nt": "TTGGTTCTTGGAAAAAGAGCAAATGGCTATAAAAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGCGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGA", "aa": "MVLGKRANGYKNILTLTERKTRVGFATFVSSKSPFEINSKIKELIKNKNLIVKTITIDNGIEFEKIGFLAKSLNIIIYRAEPYASFQRGSNEN", "aa_hexdigest": "151ece11318bfe9f9904904a28af0e1a", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001F20DEBE", "query_cov": 0.978494623655914, "subject_cov": 0.8272727272727273, "identity": 0.67, "score": 125.0, "evalue": 1.07e-35, "valid": false, "product": "IS30 family transposase", "uniref50_id": "UniRef50_UPI001F20DEBE", "go_ids": [ "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F20DEBE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "product": "IS30 family transposase" }, "genes": [], "id": "DFOKDJOJIG_636", "locus": "GCA013367855_00620" }, { "type": "cds", "sequence": "contig_1", "start": 350101, "stop": 350211, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAGAAGAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGA", "aa": "MIRREYQKGFNFNILTQEDLDTTIAKINNMPREILN", "aa_hexdigest": "8ef7b9776e982e4522979ce89f78742f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4340.9349, "isoelectric_point": 6.038881492614746 }, "id": "DFOKDJOJIG_637", "locus": "GCA013367855_00621" }, { "type": "cds", "sequence": "contig_1", "start": 350642, "stop": 350980, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAATTTTTAGAAAATCTTCATTTAATTCACTTTATCATAAAGTTTTAACGATCATGTCTAATACACATTGTTCATTTGAAGAAAAAATGGAAACAATTAAAAGAGTTTTATTAAGCAAAGGAATTAATCTAGTAACGATGCCTTTTATTGAAAATAGTTTGATAAGGGCTATTGCTTTGAAAAAGGGAGCAAAAAGGTATATTTTCCTTTCTGATATGTTTAATTCTGAAGCATATATATTTTATTCATTGCTACATGAAATAGTTCATTGCTTTTTTTCAAATTATACTGAAAACAAAATTGATAAAGTTGTTATCAAAGAATATTTTGCATGA", "aa": "MGIFRKSSFNSLYHKVLTIMSNTHCSFEEKMETIKRVLLSKGINLVTMPFIENSLIRAIALKKGAKRYIFLSDMFNSEAYIFYSLLHEIVHCFFSNYTENKIDKVVIKEYFA", "aa_hexdigest": "855065ba34c2652e9f42b51014bff420", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13147.340199999984, "isoelectric_point": 9.274824714660646 }, "id": "DFOKDJOJIG_638", "locus": "GCA013367855_00622" }, { "type": "cds", "sequence": "contig_1", "start": 351059, "stop": 351541, "strand": "-", "frame": 3, "gene": null, "product": "HTH cro/C1-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J844", "UniRef:UniRef90_D1J844" ], "nt": "ATGATTGAAGAGCACATCTGTGATATGAAGCACGTTAAAATTCATGGCAATGATTTTACAGATGAAATAAAATACTATTTAAAAATGTTTTCAATGACTCAAAAAGAATTATCTATAAGACTTGGATTATCAATAAAACATATCAATTCAATTATGAATAATGAAGTTAATAACGTTAGCGCAACTATTATTGAAGCGCTTGAATATGCATTTCACTTAGAAATTGGCACATTAACGGAAGTTTATCATATTTATAATAACTTAAAAACACTAAAGAAAAATAAGAATATTGAAGATGATTTAAAGAAACATGGTATTGATTTTTTAATAAATCATCCAGAGCTAGCATTAACAGCTAACATTAGCATTCATGAAAATAGTCCATTACATGTTAAATTAATAATGCTAAAAAGATTTTTTGGTGTTGCTGACTTGGCTAACTATGATAAATATTTAAAAGATAATGTTTCAGCAGAAGAATGA", "aa": "MIEEHICDMKHVKIHGNDFTDEIKYYLKMFSMTQKELSIRLGLSIKHINSIMNNEVNNVSATIIEALEYAFHLEIGTLTEVYHIYNNLKTLKKNKNIEDDLKKHGIDFLINHPELALTANISIHENSPLHVKLIMLKRFFGVADLANYDKYLKDNVSAEE", "aa_hexdigest": "8a87fc387dc68313e5539db5f728a837", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 350756, "stop": 351541, "observations": { "mutation": [ 351031, 351061 ], "stop": [ 351031, 351061 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J844", "query_cov": 1.0, "subject_cov": 0.4456824512534819, "identity": 0.9940000000000001, "score": 321.0, "evalue": 3.84e-108, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MIEEHICDMKHVKIHGNDFTDEIKYYLKMFSMTQKELSIRLGLSIKHINSIMNNEVNNVSATIIEALEYAFHLEIGTLTEVYHIYNNLKTLKKNKNIEDDLKKHGIDFLINHPELALAANISIHENSPLHVKLIMLKRFFGVADLANYDKYLKDNVSAEEWTYSNPNTKVWIRFCELLSIDKNIDENVGIFRKSSFNSLYHKILTIMSNTHCSFEEKMETIKKVLLSKGINLVTMPFIENSLIRAIALKKGAKRYIFLSDMFNSEAYIFYSLLHEIVHCFFSNYTENKIDKVVIKEYFAWEKNNPTTYKAIYDAINSYQQCEAIQKANKNIDTSYIWSTLKDKYPYVAFDPLSDQKSKN" }, "paralog": false, "identity": 0.9808429118773946, "score": 509.0, "evalue": 9.98e-185, "description": "Internal stop codon at 351031, 351061. Nonsense mutation around 351031, 351061.", "psc": { "uniref90_id": "UniRef90_D1J844", "product": "HTH cro/C1-type domain-containing protein", "uniref50_id": "UniRef50_D1J844", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J844", "UniRef:UniRef50_D1J844" ] }, "pscc": { "uniref50_id": "UniRef50_D1J844", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J844" ], "product": "HTH cro/C1-type domain-containing protein" } }, "id": "DFOKDJOJIG_639", "locus": "GCA013367855_00623" }, { "type": "cds", "sequence": "contig_1", "start": 351715, "stop": 352110, "strand": "-", "frame": 1, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0008270", "GO:0009972", "KEGG:K01489", "RefSeq:WP_036438513.1", "SO:0001217", "UniParc:UPI0005144531", "UniRef:UniRef100_A0A454C9K2", "UniRef:UniRef50_D5E4U5", "UniRef:UniRef90_A0A454C9K2" ], "nt": "ATGACAATAGAAGAATTAAAAAATAAATTAAAATATTCATTTAGTCCTTATTCACATGTTCAAGTTGCATGTTGTGCAATTGAAGAAAATGGTAAAGAATATTGGGGAGTCAATACTGAAAACGCTGCATTTCCATCTGGATTATGTGCTGAAAGAAGTGCATTATTTGGTTCTGTAGCATATGGCGCTAGAGTTGGTAATTTTAAAGAAATACATATAATTAGCAATTTATCTAAATTGCTATATCCTTGCGGTGCTTGTGCCCAAGTTATTAGCCAATTTTTAAAACCAGATGGCGATGTATATTTATACAGTTTTGATTTAAAGCAACAAAAGCATTATAAAATGAAAGATATTCTACCTTTTGCAGTTGCACCAAAAGATATAGTGGATTAG", "aa": "MTIEELKNKLKYSFSPYSHVQVACCAIEENGKEYWGVNTENAAFPSGLCAERSALFGSVAYGARVGNFKEIHIISNLSKLLYPCGACAQVISQFLKPDGDVYLYSFDLKQQKHYKMKDILPFAVAPKDIVD", "aa_hexdigest": "675e8b3d310036b1f9d0a200199bd6cb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0005144531", "ncbi_nrp_id": "WP_036438513.1", "uniref100_id": "UniRef100_A0A454C9K2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005144531", "RefSeq:WP_036438513.1", "UniRef:UniRef100_A0A454C9K2" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9K2", "uniref90_id": "UniRef90_A0A454C9K2" }, "psc": { "uniref90_id": "UniRef90_A0A454C9K2", "gene": "Cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_D5E4U5", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0008270", "GO:0009972" ] }, "pscc": { "uniref50_id": "UniRef50_D5E4U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E4U5" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "DFOKDJOJIG_640", "locus": "GCA013367855_00624" }, { "type": "cds", "sequence": "contig_1", "start": 352262, "stop": 352519, "strand": "+", "frame": 2, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "RefSeq:WP_036432541.1", "SO:0001217", "UniParc:UPI000513536C", "UniRef:UniRef100_A0A2K9YTE6", "UniRef:UniRef50_B3PMA1", "UniRef:UniRef90_D1J846" ], "nt": "ATGGTTAAATTAAGATTAAAAAGAACTGGTAAAAAATTCTATGCAACATATAAAATTGTTGCCGCAGATGCAAGAAGCCCACGTGATGGTAAATTTATTGAAGAATTAGGCTACTATGATCCTAATTCAAAAAAATTAGTGTTAAATAAGGAATTAATTATTAAACACTTGAACAACGGTGCAAAACCTTCAGACACTGTACGTAATTTGTTAAAGCAAGACAACTTCTATGCAGACTATGTTGCTTCTAAAAAATAA", "aa": "MVKLRLKRTGKKFYATYKIVAADARSPRDGKFIEELGYYDPNSKKLVLNKELIIKHLNNGAKPSDTVRNLLKQDNFYADYVASKK", "aa_hexdigest": "a7a483f38af834dbe4316c42c22e2626", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000513536C", "ncbi_nrp_id": "WP_036432541.1", "uniref100_id": "UniRef100_A0A2K9YTE6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513536C", "RefSeq:WP_036432541.1", "UniRef:UniRef100_A0A2K9YTE6" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTE6", "uniref90_id": "UniRef90_D1J846" }, "psc": { "uniref90_id": "UniRef90_D1J846", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_B3PMA1", "cog_id": "COG0228", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMA1" ], "product": "Small ribosomal subunit protein bS16" }, "genes": [ "rpsP" ], "id": "DFOKDJOJIG_641", "locus": "GCA013367855_00625" }, { "type": "cds", "sequence": "contig_1", "start": 352532, "stop": 353134, "strand": "+", "frame": 2, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "db_xrefs": [ "COG:COG0336", "COG:J", "EC:2.1.1.228", "GO:0008033", "GO:0052906", "KEGG:K00554", "SO:0001217", "UniRef:UniRef50_A9NHF7", "UniRef:UniRef90_D1J847" ], "nt": "ATGAAGATAAACATTCTGACACTATTTCCAGATTATTTAAATGCTTTTTGCAATCAAAGTATGATAGCAAAAGCATTAGAGCTTGGACACATTGAAATAAACATCATTAATTTTAGAGATTATTCATTAGATAAACATCATAAAGTTGATGATAGCCCTTATGGTGGAGGCGCAGGTATGTTGTTGCAAATTGAACCAATAGATTTAGCACTACAGCCTTTAAAAAATAGTCACAAAATTTTACTTTCACCGCAAGGAAAGGTATTTAGTCAAAAAAAAGCACATGAACTTGCCAAATTAAATGAAATTACTTTAATATGTGGGCATTATGAAGGCTTTGATGAAAGAATAACAAAATTAGTGGATGAAGAAATATCAATTGGAGATTTTATCTTAACAGGTGGAGAAATTGCAGCAATGGCAATTTCAGAAGCAGTAGCAAGATTAATACCAGGAGTATTAAGAAAAAATAGCTATGAAAATGAAAGCTTCGAAGGAAATGGATTACTAGATTATCCACAATACACAAGACCTGCCGTATATAAGGGAATGAAAGTACCAAAAATCTTATTAAGTGGAAATCACCTAGAAATAGAAAAATGA", "aa": "MKINILTLFPDYLNAFCNQSMIAKALELGHIEINIINFRDYSLDKHHKVDDSPYGGGAGMLLQIEPIDLALQPLKNSHKILLSPQGKVFSQKKAHELAKLNEITLICGHYEGFDERITKLVDEEISIGDFILTGGEIAAMAISEAVARLIPGVLRKNSYENESFEGNGLLDYPQYTRPAVYKGMKVPKILLSGNHLEIEK", "aa_hexdigest": "5267e1237e2a3031a4db6e05b9bc9830", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J847", "query_cov": 1.0, "subject_cov": 0.9009009009009009, "identity": 0.995, "score": 400.0, "evalue": 7.73e-141, "valid": true, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_A9NHF7", "cog_id": "COG0336", "cog_category": "J", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_A9NHF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A9NHF7" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [ "trmD" ], "id": "DFOKDJOJIG_642", "locus": "GCA013367855_00626" }, { "type": "cds", "sequence": "contig_1", "start": 353202, "stop": 353558, "strand": "+", "frame": 3, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "COG:COG0335", "COG:J", "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_012855536.1", "SO:0001217", "UniParc:UPI0001BC2235", "UniRef:UniRef100_D1J848", "UniRef:UniRef50_Q044E2", "UniRef:UniRef90_D1J848" ], "nt": "ATGAGAACAAAATTGTTAGAATTAGTAGAAAAAGATCAAATAAGAGAAGACTTGCCACAATTAAGAGAAGGTTATAACGTAAGAGTTCACGTAAGAATTAAAGAAGGCGATAAAGAAAGAATTCAAGTTTTTGAAGGATTAATTATTGCTGAATATGGTACAGGAATCAATAAATCAATTACCGTAAGAAAAGAATCATATGGTGTTGGAGTTGAAAGAATCTTTAAAATTAACTCACCTTTAATTGCTCAAATCGAAGTTATTAGACGCAATAAAGTAAGACGTGCTAAATTGTATTACATGAGAGATTTAAAAGGTAAGAGTGCTAGATTAAAAGAAATTAAAAACTCAAAATAA", "aa": "MRTKLLELVEKDQIREDLPQLREGYNVRVHVRIKEGDKERIQVFEGLIIAEYGTGINKSITVRKESYGVGVERIFKINSPLIAQIEVIRRNKVRRAKLYYMRDLKGKSARLKEIKNSK", "aa_hexdigest": "9c890839eeb918280e6bf122bb6ab676", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2235", "ncbi_nrp_id": "WP_012855536.1", "uniref100_id": "UniRef100_D1J848", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2235", "RefSeq:WP_012855536.1", "UniRef:UniRef100_D1J848" ] }, "ips": { "uniref100_id": "UniRef100_D1J848", "uniref90_id": "UniRef90_D1J848" }, "psc": { "uniref90_id": "UniRef90_D1J848", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_Q044E2", "cog_id": "COG0335", "cog_category": "J", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q044E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q044E2" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "DFOKDJOJIG_643", "locus": "GCA013367855_00627" }, { "type": "cds", "sequence": "contig_1", "start": 353768, "stop": 353980, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATACAAAAATTTTATTAGCATTAGGAAGCATTGCAACTTCAGTTGCTGCAATACCATTAGTTGCTGCAGGTTGCGTAAAAACAAAAAAACCAGAAGTTAAACCAGAAGAACCTAAAAAACCAGAAGGTGAAAAACCAGGAACAACACCAGAAGTTAAACCAGAAGATAATAGATCAGAAGAACAAAAAGAAATAGATAATTGA", "aa": "MKKHTKILLALGSIATSVAAIPLVAAGCVKTKKPEVKPEEPKKPEGEKPGTTPEVKPEDNRSEEQKEIDN", "aa_hexdigest": "32d92ef490036a72b794e36b133cf318", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7560.657399999999, "isoelectric_point": 7.835374641418458 }, "id": "DFOKDJOJIG_644", "locus": "GCA013367855_00628" }, { "type": "cds", "sequence": "contig_1", "start": 354910, "stop": 355089, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTGTTATTTTAATTCAAATGTTTGTGAACCAGTTTTAGTTCCAAAGATAAAAGGTTCTACTCCATTATCTTTTTGTCCACCAGCTCAAGTTATAGTAATTTTATTATCTTTTACTTCATATTCTAAGCAGTTAGATAATTCTAATTTATCTTTTGCAACTTTTTCATCTTTGTAG", "aa": "MFCYFNSNVCEPVLVPKIKGSTPLSFCPPAQVIVILLSFTSYSKQLDNSNLSFATFSSL", "aa_hexdigest": "b28463d095b9ca3a711893a69c6912dd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6495.515099999999, "isoelectric_point": 7.698693656921387 }, "id": "DFOKDJOJIG_645", "locus": "GCA013367855_00629" }, { "type": "cds", "sequence": "contig_1", "start": 355146, "stop": 355556, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22421.1" ], "nt": "ATGACTCAACACAATGAAAATAAAACCAAAAGAATTGCCCAAAAAAAATTAGCTTTTGAATTGGTTAAAGACATTCATGGAGTTGATATTGCAAACAAATGTATTAGAATAAGTGAGATTTTATTCAATAAAAGTAACGAAGAATTAAACATAGAAGATATTTCGATGCTTTTAGGCTATTTGCCATGTGTTGAAGTTAAAGAAGGCGAAAAATTTATTGAAATTTTAAAAAATAATCAAATTGTTTCTTCAAATAGAGAAGCAAGAGAATTTATTTCAAAAAATACTTTTTTAATTGATAACAATATAATAAACGATGAAAATTATTCAATTACATTTAATAAATTTAATGGTCAATATGCGCTATTAAAAAAAGGCAAAAAAGATTTTTACATACTTAAAAGAATTTAA", "aa": "MTQHNENKTKRIAQKKLAFELVKDIHGVDIANKCIRISEILFNKSNEELNIEDISMLLGYLPCVEVKEGEKFIEILKNNQIVSSNREAREFISKNTFLIDNNIINDENYSITFNKFNGQYALLKKGKKDFYILKRI", "aa_hexdigest": "cfc729703bbc6ca4042e330c718d53fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 81, "aa_cov": 0.5808823529411765, "hmm_cov": 1.0, "evalue": 1.6628480240037976e-14, "score": 49.20185852050781, "start": 54, "stop": 132 } ], "seq_stats": { "molecular_weight": 15900.178199999984, "isoelectric_point": 8.798983573913574 }, "id": "DFOKDJOJIG_646", "locus": "GCA013367855_00630" }, { "type": "cds", "sequence": "contig_1", "start": 355593, "stop": 355751, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTTAATTTATTAGTTGATGAAAATGGCAAAAAATTTGGTAAGAGCACTGGCGGAGGTTCATTATGGCTTGATCCAAATATGACTAGCCCATATTCAATATATCAATTTTTATTAAATCAAAGTGATGTAAAAATTGAAGAATATTTAAAATGA", "aa": "MTFNLLVDENGKKFGKSTGGGSLWLDPNMTSPYSIYQFLLNQSDVKIEEYLK", "aa_hexdigest": "4f8361fe08f003b759ad8e2e669c4a83", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5918.6582, "isoelectric_point": 4.992987251281736 }, "id": "DFOKDJOJIG_647", "locus": "GCA013367855_00631" }, { "type": "cds", "sequence": "contig_1", "start": 356016, "stop": 356381, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEF080B" ], "nt": "ATGCAAAAAAATATATTAGATGATTTGAAATCAAGGGGTGTTTTAAATAATATATCTAGTGAAGAAAAATTTAATAATATATCACTAGGGACAGGTGTTTATACAGGCTTTGATCCAACTGCTAAAAGTTTACACCTTGGAAATTATCTACAAATATTAAGTTTGTTACGTTTTAAAAAAGCAGGATACAAGCCAATTGCAATATTGGGTGGAATAACCGGAATGATTGGTGATCCTAGTTTTAGAAATTCTGAAAGAAAATTTCTAGATCAAGAAACACTACTACAAAACAAAAATGCAATTAAAAATCAATTACAAAAATTTGGTTTAGAAGTAATTGATAATTTAGATTTTTATAAACATTGA", "aa": "MQKNILDDLKSRGVLNNISSEEKFNNISLGTGVYTGFDPTAKSLHLGNYLQILSLLRFKKAGYKPIAILGGITGMIGDPSFRNSERKFLDQETLLQNKNAIKNQLQKFGLEVIDNLDFYKH", "aa_hexdigest": "4fd057b6db23b16aef4ac9231c5c4af3", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002FEF080B", "query_cov": 0.9752066115702479, "subject_cov": 0.9752066115702479, "identity": 0.6609999999999999, "score": 157.0, "evalue": 2.11e-47, "valid": false, "product": "tRNA synthetases class I (W and Y)", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_UPI002FEF080B", "kegg_orthology_id": "K01866" }, "pscc": { "uniref50_id": "UniRef50_UPI002FEF080B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEF080B" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13633.498899999982, "isoelectric_point": 9.362501716613771 }, "id": "DFOKDJOJIG_648", "locus": "GCA013367855_00632" }, { "type": "cds", "sequence": "contig_1", "start": 356715, "stop": 357386, "strand": "+", "frame": 3, "gene": "degV", "product": "DegV family protein", "db_xrefs": [ "COG:COG1307", "COG:I", "SO:0001217", "UniRef:UniRef50_A0A077L663", "UniRef:UniRef90_D1J851" ], "nt": "ATGCAAGTTATAGAAAAATATTTTAATGACTACGATAAAATTTTAGTCTATCCAATTTCAAATGCGCTTTCTTCGCAATGTGCTTCACTAACAGCACAATTTGCTTCAAATCCAAAAGTTCATGTAGTACAATCAAATAAAATTTCTTATCTGATTGTTAGAGACTTATTAATGTTTGAAGAATATATTAAAGAAGGAAAAAGCTTCGATGAGGCAATAGAACACTTTAAAAATAATAATGAAAGATTACTTCTAATTCCGGAGTTTAACGATGCCTTACTAAAAGGCGGACGTTTATCAAAAGCAGCAGCCGCAATAGCAAAATTATTAAAAATAGTTCCTATTATTAAATTTGATAACGGAATTTTAGAAAAAGATGGTATTGGTAGAATCTTTAAAAAAACTCTAGAAAAAAATTTAAAAGAATTATATGAAAATACAAACAAAGATAATGAAAACAATTATCTATTAGTTGTAAATGCAAATAACGATAAAATAGATGAATTAATTCAGTCGTTTAAACAAATAACCAATAATTTTCCAAGGATTTATACAATTCAATTGAGCTGCGACATTGTAGTTCATGCTGGTATTGGCGCAATCTGTTTAACATTTGCCGAAGTTAGTCCTAAAATAAAAGATAAATTCTTTTTATACTTAAAAAAACAATAA", "aa": "MQVIEKYFNDYDKILVYPISNALSSQCASLTAQFASNPKVHVVQSNKISYLIVRDLLMFEEYIKEGKSFDEAIEHFKNNNERLLLIPEFNDALLKGGRLSKAAAAIAKLLKIVPIIKFDNGILEKDGIGRIFKKTLEKNLKELYENTNKDNENNYLLVVNANNDKIDELIQSFKQITNNFPRIYTIQLSCDIVVHAGIGAICLTFAEVSPKIKDKFFLYLKKQ", "aa_hexdigest": "f2407cbe3a1718095f5c23dcbc34070f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "degV" ], "truncated": "5-prime", "pseudogene": { "start": 356505, "stop": 357386, "observations": { "mutation": [ 356571, 356658 ], "stop": [ 356571, 356658 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J851", "query_cov": 1.0, "subject_cov": 0.7610921501706485, "identity": 1.0, "score": 432.0, "evalue": 3.96e-152, "gene_start": 1, "gene_end": 223, "reference_start": 71, "reference_end": 293, "reference_sequence": "MKIKIITDSSAGMSQEQANSFGWDLIPIQIEVGNDIYKSGIDFDTDKFEELWRNDKKIDAKTFATPPGIAMQVIEKYFNDYDKILVYPISNALSSQCASLTAQFASNPKVHVVQSNKISYLIVRDLLMFEEYIKEGKSFDEAIEHFKNNNERLLLIPEFNDALLKGGRLSKAAAAIAKLLKIVPIIKFDNGILEKDGIGRIFKKTLEKNLKELYENTNKDNENNYLLVVNANNDKIDELIQSFKQITNNFPRIYTIQLSCDIVVHAGIGAICLTFAEVSPKIKDKFFLYLKKQ" }, "paralog": false, "identity": 0.9897610921501706, "score": 567.0, "evalue": 1.06e-207, "description": "Internal stop codon at 356571, 356658. Nonsense mutation around 356571, 356658.", "psc": { "uniref90_id": "UniRef90_D1J851", "gene": "DegV", "product": "DegV family protein", "uniref50_id": "UniRef50_A0A077L663", "cog_id": "COG1307", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J851", "UniRef:UniRef50_A0A077L663", "COG:COG1307", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L663" ], "product": "DegV family protein" } }, "id": "DFOKDJOJIG_649", "locus": "GCA013367855_00633" }, { "type": "cds", "sequence": "contig_1", "start": 357427, "stop": 357690, "strand": "-", "frame": 1, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "COG:COG0211", "COG:J", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "RefSeq:WP_012855540.1", "SO:0001217", "UniParc:UPI0001BC2239", "UniRef:UniRef100_D1J852", "UniRef:UniRef50_O21275", "UniRef:UniRef90_D1J852" ], "nt": "ATGGCACATACGAAATCAGGTGGAACAACCGCCAATAGCCGTGATTCAGCGGGAAGAAGATTAGGCGTAAAAGCAACCGACGGCCAATTTATTTTAGCAGGAAGCATTATTTATAGACAAAGAGGAACAAAAATTTTCCCAGGCCAAAATGTTGGTAGAGGAAAAGATGACACCTTATTTGCTTTAATCGATGGTATTGTAAAATTCGAAAGCAGAATTAACAGAAAATTTGCATCAGTTTACCCAATTGAAAGCAAGAAATAA", "aa": "MAHTKSGGTTANSRDSAGRRLGVKATDGQFILAGSIIYRQRGTKIFPGQNVGRGKDDTLFALIDGIVKFESRINRKFASVYPIESKK", "aa_hexdigest": "8bf9087f30d2afc010cb018fb10ca22d", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2239", "ncbi_nrp_id": "WP_012855540.1", "uniref100_id": "UniRef100_D1J852", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2239", "RefSeq:WP_012855540.1", "UniRef:UniRef100_D1J852" ] }, "ips": { "uniref100_id": "UniRef100_D1J852", "uniref90_id": "UniRef90_D1J852" }, "psc": { "uniref90_id": "UniRef90_D1J852", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_O21275", "cog_id": "COG0211", "cog_category": "J", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "genes": [ "rpmA" ], "id": "DFOKDJOJIG_650", "locus": "GCA013367855_00634" }, { "type": "cds", "sequence": "contig_1", "start": 357693, "stop": 357995, "strand": "-", "frame": 2, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855541.1", "SO:0001217", "UniParc:UPI0001BC223A", "UniRef:UniRef100_D1J853", "UniRef:UniRef50_Q6F123", "UniRef:UniRef90_D1J853" ], "nt": "ATGTTTGCTATTATTGAAACCGGCGGAAAACAATTACTTGTTAAAAGCGGTGATGTAATTTACATCGAAAAAATTGATGGCAACGAAGGTGACAAAGTTTCATTTGATAAAGTTTTAGTTATTGATGAACAAGTTGGCTCACCTTATCTACCAAAAGCTTCTGTTTTAGGAACCATCGAAAAACAAGGAAAAGCAAAAAAAATAGTTGTTTATAGACATAATGCTAAATCTACTCATAAACGTAAATTAGGACATCGTCAACCATTTACCAGAGTTAAAATTACAGAAATTAAGGAAAAATAG", "aa": "MFAIIETGGKQLLVKSGDVIYIEKIDGNEGDKVSFDKVLVIDEQVGSPYLPKASVLGTIEKQGKAKKIVVYRHNAKSTHKRKLGHRQPFTRVKITEIKEK", "aa_hexdigest": "2e1b8b13d765a237bc4b0b6090d9d3bf", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC223A", "ncbi_nrp_id": "WP_012855541.1", "uniref100_id": "UniRef100_D1J853", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC223A", "RefSeq:WP_012855541.1", "UniRef:UniRef100_D1J853" ] }, "ips": { "uniref100_id": "UniRef100_D1J853", "uniref90_id": "UniRef90_D1J853" }, "psc": { "uniref90_id": "UniRef90_D1J853", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_Q6F123", "cog_id": "COG0261", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6F123", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6F123" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "DFOKDJOJIG_651", "locus": "GCA013367855_00635" }, { "type": "cds", "sequence": "contig_1", "start": 358531, "stop": 358638, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATTAAAGAAAAATTAAATGGAATAAAAGAAGGCTTTAAAATAGGCAGTGACAAATTAAAAAATAAAAAAAAGATCTATAAAATAGATTTTCATACTCTATAA", "aa": "MEIKEKLNGIKEGFKIGSDKLKNKKKIYKIDFHTL", "aa_hexdigest": "581a6e2a1b01e4500804cba0e8919f72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4135.912300000001, "isoelectric_point": 9.757306098937985 }, "id": "DFOKDJOJIG_652", "locus": "GCA013367855_00636" }, { "type": "cds", "sequence": "contig_1", "start": 359363, "stop": 359638, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAATTACATTAAAATGGTTAACAATTTTAGGAGCAACAACGCTTACTGCCTTGCCTTTTTGTGCAATTTCTTGTAGTAATGAGGAAAAAGAAATTGAAAAAGCAGAGAACATTATTGGCGATAAAGAACTAACATTTAAAATAGATGATTTAAAATATGAAAATTCTGATAATTCTATATTGCAAAGTGCTAGAAGTATTGCAGGACCATGGCGCAAGGACTTTCTTAAGGTTGTTATAACGTCAAAATCAAATATTGAAGAATATTGA", "aa": "MKKITLKWLTILGATTLTALPFCAISCSNEEKEIEKAENIIGDKELTFKIDDLKYENSDNSILQSARSIAGPWRKDFLKVVITSKSNIEEY", "aa_hexdigest": "f926b59fb9909298560fa834b6a393d9", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10311.767999999991, "isoelectric_point": 5.447187614440917 }, "id": "DFOKDJOJIG_653", "locus": "GCA013367855_00637" }, { "type": "cds", "sequence": "contig_1", "start": 359744, "stop": 360067, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U0", "UniRef:UniRef90_A0A454C9U0" ], "nt": "ATGGATCTAAAATCTTCTTACCAAAAAGCAAACAAAGCATATTTAATAGAAAAACCAAAAAAAGTATATTCAAATCTAAAAAATAAACAAATATTAATAAATAACTACACTATATTTTTTGATGATATAAATAATGATAAATCTCCAAGAGTTTCAATGGCGATAAAAGAATTAAAATTTAATTTTGATCAAGACCGAAAAACCATAGAAATTAAACTTGGTTTAAGATATGAATTATCACAAAAACATAATAGGAAGTCTAATATATTTTTTCTTTCTCCTAAGGCAACCTTTAAATACATTTTAAGCGAAGAAATCAAATAA", "aa": "MDLKSSYQKANKAYLIEKPKKVYSNLKNKQILINNYTIFFDDINNDKSPRVSMAIKELKFNFDQDRKTIEIKLGLRYELSQKHNRKSNIFFLSPKATFKYILSEEIK", "aa_hexdigest": "3ae9af9080292910bf3cae1e53afa526", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 359444, "stop": 360067, "observations": { "mutation": [ 359636, 359648 ], "stop": [ 359636, 359648 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9U0", "query_cov": 1.0, "subject_cov": 0.45726495726495725, "identity": 0.981, "score": 201.0, "evalue": 9.53e-64, "gene_start": 1, "gene_end": 107, "reference_start": 128, "reference_end": 234, "reference_sequence": "MKKITLKWLTILGATTLTALPFCAISCSNEEKEIEKAEKIIGDKELTFKIDDLKYENSDNSILQSARSIAGPWRKDFLKVVITSKSNIEEYWKDNWIVGKIDLKNKAILFLIPREVLLDADFIKEKNMDLKSSYQKANKAYLIEKPKKVYSNLKNKQIWINNYTIFFDDINNDKSPRVSMAIKELKFNFDQDQKTIEIKLGLRYELSQKHNRKSNIFFLSPKATFKYILSEEIK" }, "paralog": false, "identity": 0.9758454106280193, "score": 393.0, "evalue": 9.18e-142, "description": "Internal stop codon at 359636, 359648. Nonsense mutation around 359636, 359648.", "psc": { "uniref90_id": "UniRef90_A0A454C9U0", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A454C9U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9U0", "UniRef:UniRef50_A0A454C9U0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U0" ], "product": "Lipoprotein" } }, "id": "DFOKDJOJIG_654", "locus": "GCA013367855_00638" }, { "type": "cds", "sequence": "contig_1", "start": 360260, "stop": 360517, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_020002320.1", "SO:0001217", "UniParc:UPI00037C7492", "UniRef:UniRef100_UPI00037C7492", "UniRef:UniRef50_A0A454C9K9", "UniRef:UniRef90_A0A454C9K9" ], "nt": "ATGCAGCAAGAGGCTAAATTAAACAACGATTATTCAAAGCTCTTTAATTCTTATAAAAACCAAATTAAGAATTTAAATAATTTAAACATCGAAAATAAATTTCTAAAACAAAGAATTTTAAAAATTATAAATAATGAAATAAATGATTTTAAAAAAGTTGGTAATAAGATAATTAAAACCTTACTAAATGCCTATAAGCAAAACAATAATGCATTTACTGGCCAAAAGAATTTCGCTTCATTTTTTAATGAGTTTTAA", "aa": "MQQEAKLNNDYSKLFNSYKNQIKNLNNLNIENKFLKQRILKIINNEINDFKKVGNKIIKTLLNAYKQNNNAFTGQKNFASFFNEF", "aa_hexdigest": "b029b4b7145826ac8865b2d24045e00b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00037C7492", "ncbi_nrp_id": "WP_020002320.1", "uniref100_id": "UniRef100_UPI00037C7492", "db_xrefs": [ "SO:0001217", "UniParc:UPI00037C7492", "RefSeq:WP_020002320.1", "UniRef:UniRef100_UPI00037C7492" ] }, "ips": { "uniref100_id": "UniRef100_UPI00037C7492", "uniref90_id": "UniRef90_A0A454C9K9" }, "psc": { "uniref90_id": "UniRef90_A0A454C9K9", "uniref50_id": "UniRef50_A0A454C9K9" }, "pscc": { "uniref50_id": "UniRef50_A0A454C9K9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9K9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10093.408799999988, "isoelectric_point": 9.890175437927244 }, "id": "DFOKDJOJIG_655", "locus": "GCA013367855_00639" }, { "type": "cds", "sequence": "contig_1", "start": 360621, "stop": 361172, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTCTAAATTAGTAATTTCTGAACCCGGAGATTCTAAATTCTTCGCTGGTGATATTGTAGATATTTTTGACTATCAAGAAGAAAATGCTATTCTACTTTCAAAAGGTTTGAAGCCAGCATATGGTAAAGTTGTTATTAAAGGTGCAAAACAAGTTCCATTGTTGTCTGATTCATTCCTTGCTGCCGCTTCTTATCAAGAAACAAGCAAGATATTAGTTCACGCTGCTATTTCATCAAGAATTGACAATTTATCTGGCTTAAAAGAAAATATTATTCTTGGTAAAAAGATTCCATCAGGAACAGCATTATTCCCATTTGAAGAACATTCAAAATATGATATAAAACCATCAATTAATTACTTTACAACAACTCCTATTGACGGCACAGAAATTTCTGAACCTATTGATATTGATTCAGTTGTTAATACAGCGCAAGAATATGACAATGATAAACAATCAGTTGAAGAAGATTATTCTCCAAACGACGAAGAACTTGTTGCTGAAGAAGAAACATTTGATGATGAAGAAAATCAAAACGATTTTGAATAA", "aa": "MMSKLVISEPGDSKFFAGDIVDIFDYQEENAILLSKGLKPAYGKVVIKGAKQVPLLSDSFLAAASYQETSKILVHAAISSRIDNLSGLKENIILGKKIPSGTALFPFEEHSKYDIKPSINYFTTTPIDGTEISEPIDIDSVVNTAQEYDNDKQSVEEDYSPNDEELVAEEETFDDEENQNDFE", "aa_hexdigest": "0ac3ae55a72f2a0b41d361e9ddd65c27", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20309.21869999999, "isoelectric_point": 4.22878704071045 }, "id": "DFOKDJOJIG_656", "locus": "GCA013367855_00640" }, { "type": "cds", "sequence": "contig_1", "start": 361518, "stop": 362213, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04998.22" ], "nt": "ATGGCTAACAATACTAAAACTACTAAGGCCGATGCTAAAAACGACCGTGTTGTTAGATCAACAGTTGAAGTTCCTGTTAAGTCTTCATTTATTGAAGGACTTACTGCATTTGAATTCTATTCATCAACTCACGGTGCTAGAAAAGGTTTAACCGATACTGCTTTGAACACTGCAAAATCTGGTTATTTAACAAGACGTTTAGTTGATGTTGCTCAAAATATTGTTGTTAGAGAAGAAAACTGTGGTACTGACTATGGTTATAAAGCAAGAAACATAGTTGATACAAAAACTAAACAAATTATTGTTCCTTTGAAAGAAAGAATTGTTGGAAGATTTGCAAACAAACCTGTTGTTACAAGTGCGGGTATAGAAATTTGTTCTAGAAATGAATTAATTACTGAAAAAATTGCTCAACGAATAATAGCAAGTGGTATTGAAGAAGTCGAAATTAGATCAATCTTAGGCTGCAATACACGTAACGGTGTATGTAAGATGTGTTTTGGTAAAGACCTTGCAACAAATAGAATTGTTAATATCGGTGAAGCAGTAGGTATTATTGCAGCTCAATCTATTGGGGAACCAGGAACTCAATTAACTATGCGTACATTCCACACTGGTGGTGTTGCTGGGGTTGAAGATATTACTGGTGGTTTCACTAGATTGATTGAATTAATTGATGCCCACGAACAACCATGA", "aa": "MANNTKTTKADAKNDRVVRSTVEVPVKSSFIEGLTAFEFYSSTHGARKGLTDTALNTAKSGYLTRRLVDVAQNIVVREENCGTDYGYKARNIVDTKTKQIIVPLKERIVGRFANKPVVTSAGIEICSRNELITEKIAQRIIASGIEEVEIRSILGCNTRNGVCKMCFGKDLATNRIVNIGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGVEDITGGFTRLIELIDAHEQP", "aa_hexdigest": "6d193b5b2dc7f45a38ee5e7a1f782503", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 195, "aa_cov": 0.8441558441558441, "hmm_cov": 0.6, "evalue": 4.912475127663729e-47, "score": 156.22059631347656, "start": 33, "stop": 227 } ], "seq_stats": { "molecular_weight": 25058.37150000001, "isoelectric_point": 8.801626777648927 }, "id": "DFOKDJOJIG_657", "locus": "GCA013367855_00641" }, { "type": "cds", "sequence": "contig_1", "start": 362397, "stop": 362552, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAGTATTAAACAATTAGGTTTTGACTATTCAACATATTCTGGAATTTCTATTTCGTTTAGTGATATTCTAGAAACTAATAAAAAGCAAGAATATATTGCTCAAGGTGATGAATATATTGCTAAATTAAAACACTATTATGAACAAGGTTGA", "aa": "MDSIKQLGFDYSTYSGISISFSDILETNKKQEYIAQGDEYIAKLKHYYEQG", "aa_hexdigest": "fd1d13a9a161c1bc127e024eb4652b75", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5917.4749, "isoelectric_point": 4.749659538269043 }, "id": "DFOKDJOJIG_658", "locus": "GCA013367855_00642" }, { "type": "cds", "sequence": "contig_1", "start": 362583, "stop": 363395, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04983.23" ], "nt": "ATGGCTGTTCACGTTCCTGTTTCTGAACAAGCATTGCTTGAAAGTCGTGAATTAATGTTGGCTAATAAAAACATTCTAGGACCTAAAGATGGTGAACCTATTATTAACCCTTCTCAAGATATGGTTTTAGGTATTTATTATCTAACTATTGAAGAACCAGGAGCTAAAGGTGAAGGACGTGTTTTTGATAACTATGAACACATGCTTAGAGCATTAGAAAATAAAGTAGTTACATTGCACACTAGAATAGCATTACCTGCTTCAGAAGTTAAAACTCCAAAACTTATTTTCCCATTTGATGGTCCTCAATATATAATTTCAACAGTAGGTAAATTTATTTTTAATAATATATTCCCTAAAACATTTCCATTTATTTACGACAATAAAGTTGCCAAGGCAAATTCATTGGAAGATTATAATCAAACATTCAATGCAAAATATGTCGTTCAAGCAGGTACTAATATTCCTGAATATATCAAAAATATGCCTATTGAAGAAGCATTTAACAAAAAGAATATTTCTAAAATAATACGTTATGTTTTTGATAAATATGTTGCTCAAATTTCAATGTCTGATATTGCAGAAGTTATTAATAATTTAACTGAATCAAGTGATGCTAATGATTCAATAATTCAATTCTTGCATGTTAAAAATTATGAAAATAGAAAAATTGATTATGAACATGCAATGTTGCTAGCTCAATTTACAAGTCAAGAAAAGCAAAAGATTGAAATGTTAAATCCTGCTAGGGCAAATGGACAAGAAGTTCCACTTAGTCCAGCTGAAAAAGCAGAAATTCTAGACACTGTATGA", "aa": "MAVHVPVSEQALLESRELMLANKNILGPKDGEPIINPSQDMVLGIYYLTIEEPGAKGEGRVFDNYEHMLRALENKVVTLHTRIALPASEVKTPKLIFPFDGPQYIISTVGKFIFNNIFPKTFPFIYDNKVAKANSLEDYNQTFNAKYVVQAGTNIPEYIKNMPIEEAFNKKNISKIIRYVFDKYVAQISMSDIAEVINNLTESSDANDSIIQFLHVKNYENRKIDYEHAMLLAQFTSQEKQKIEMLNPARANGQEVPLSPAEKAEILDTV", "aa_hexdigest": "e47c54f02340f394fb31d6537a96b9d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04983.23", "name": "RNA_pol_Rpb1_3", "length": 182, "aa_cov": 0.6518518518518519, "hmm_cov": 0.8131868131868132, "evalue": 2.813620040237807e-26, "score": 87.98047637939453, "start": 24, "stop": 199 } ], "seq_stats": { "molecular_weight": 30617.709800000026, "isoelectric_point": 5.372728538513184 }, "id": "DFOKDJOJIG_659", "locus": "GCA013367855_00643" }, { "type": "cds", "sequence": "contig_1", "start": 363651, "stop": 363926, "strand": "-", "frame": 2, "gene": null, "product": "DNA-directed RNA polymerase subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "nt": "TTGGTTATTCAAAACGAACTAAGAATGATTCAAGAAGCAGTAGATGCTTTAATTGATAACCAAAGAAGAACACCTAACCCTGTTTTATCAAAAGACAATCGTCCATTTAAATCAATTTCAGATGCATTAACTGGTAAAAAAGGTCGTTTTAGACAAAATTTACTAGGAAAACGTGTTGACTATTCTGGACGTTCAGTTATTGCCGTAGGGCCTTCATTAAAAATGCACCAATGTGGTATACCAAGAGAAATGGCTGCTAAATTATTTGAACCATGA", "aa": "MVIQNELRMIQEAVDALIDNQRRTPNPVLSKDNRPFKSISDALTGKKGRFRQNLLGKRVDYSGRSVIAVGPSLKMHQCGIPREMAAKLFEP", "aa_hexdigest": "4958223e7fd21991b5516508c96ea886", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002381C6D8", "query_cov": 0.9340659340659341, "subject_cov": 1.0, "identity": 0.624, "score": 112.0, "evalue": 1.02e-30, "valid": false, "uniref50_id": "UniRef50_A0A3B9S0R9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B9S0R9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "product": "DNA-directed RNA polymerase subunit" }, "genes": [], "id": "DFOKDJOJIG_660", "locus": "GCA013367855_00644" }, { "type": "cds", "sequence": "contig_1", "start": 363948, "stop": 364634, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "TTGCTTGGTTTAAAATCTTCAAATGGTTCTGACGTGGTTAAACGTGAAGAACTAGAAAATTTAATTTATTACAAAAATCATATTGTTTTAGAAGATGGTGGCTTAAAATCATTACCTAAAAATCAAATTATTGAAATTAATGAAGCAGCAATTATTTACCGCAATGCTCTAGAAGAATTGCTTGAAAGATTTCAAGAAGGTACAGAGGAATATGATGATATTAAAGAAACCCTAGATTCTTTAATTGAAAAAGCAACATCAAAAATCGGTCAAGAATATGGAATTGATTTTTATGAATTAAACGAAGTAATTGAACATTATTCAGACGCTAAAATAGGCACTGGTGCTTTAGCTATTGAATATTTATTAAAAAACATAAATTTAGCTGAAGAAAAGCAAAAAGTAACTGATGAAATTAATCGTTTAAATGCAGAAGAAATTAATAATCCTGAACGTTTTGCCACAACTGCAAAACAAACAAGAGAAAAACTTTATAAACGTTTGCAAGTAATTAATGCATTTATTGAATCTAAACAAGATCCAACTAGCATGTTAATTTATAATTTGCCTGTTATTCCAGCAGATTTAAGACCTTTAATTCAATTAGATGGTGGTAGACACTCAACTAGCGATATTAATGAATTGTATCGTAGAGTAATTATTAGAAACAATAGATTGCAACAATGA", "aa": "MLGLKSSNGSDVVKREELENLIYYKNHIVLEDGGLKSLPKNQIIEINEAAIIYRNALEELLERFQEGTEEYDDIKETLDSLIEKATSKIGQEYGIDFYELNEVIEHYSDAKIGTGALAIEYLLKNINLAEEKQKVTDEINRLNAEEINNPERFATTAKQTREKLYKRLQVINAFIESKQDPTSMLIYNLPVIPADLRPLIQLDGGRHSTSDINELYRRVIIRNNRLQQ", "aa_hexdigest": "c57cfefce064fd45e8f993c30529a07d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 222, "aa_cov": 0.9342105263157895, "hmm_cov": 0.7207207207207207, "evalue": 1.3566339330521146e-22, "score": 76.0958480834961, "start": 16, "stop": 228 } ], "seq_stats": { "molecular_weight": 26268.37680000003, "isoelectric_point": 4.939615440368651 }, "id": "DFOKDJOJIG_661", "locus": "GCA013367855_00645" }, { "type": "cds", "sequence": "contig_1", "start": 364668, "stop": 365027, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "ATGAAAAAAATTTCAAAATACGAATCATTAGATGAAGAAAAAATTTCAAAAATTTCATTAGCATTAGCAACTCCTCAAGATGTTGAAACTTGGTCTCATGGTGAAGTTACTAAGCCAGAAACTATTAACTATAAATCATATAAGCCAGAACGTGGTGGATTATTTGATGAAATAATTTTTGGACCAATGATTGATTATCGTTGCCCTGTATGTGGTTTCAAATACAAAAAAGTTAATGAAGGAAGCTATTGTACAAGAACTGAATTATGTAAACAAGAACATGTTCAAATATTGCCTAAAATAGCACGTAGAACCCATATGGGACATATAAAACTAAATAGTCCAGTTGTTCACTTTTGA", "aa": "MKKISKYESLDEEKISKISLALATPQDVETWSHGEVTKPETINYKSYKPERGGLFDEIIFGPMIDYRCPVCGFKYKKVNEGSYCTRTELCKQEHVQILPKIARRTHMGHIKLNSPVVHF", "aa_hexdigest": "7ab28ebbdd5fde8231c32dc012a94f7b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 109, "aa_cov": 0.8991596638655462, "hmm_cov": 0.7522935779816514, "evalue": 6.0216748230088664e-24, "score": 80.53942108154297, "start": 13, "stop": 119 } ], "seq_stats": { "molecular_weight": 13760.796499999986, "isoelectric_point": 8.857262992858885 }, "id": "DFOKDJOJIG_662", "locus": "GCA013367855_00646" }, { "type": "cds", "sequence": "contig_1", "start": 365029, "stop": 366213, "strand": "-", "frame": 1, "gene": null, "product": "DNA-directed RNA polymerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352BHB6" ], "nt": "TTGGTTAAGGACGATGTTTATACATCAATTCACATTGAAGAACAATCAATTCAATTTAGACATTCAAAGGCTGGCGAAGATATTTTAACTGCAGATATTCCTAACGTTTCAAACTATTCTAAACGTTTCTTAGGTGAAAATGGAATTGTTAGAGTTGGTTCAGAAGTAACAGCAGGTGATATTTTAGTCGGAAGAACTTCTCCAAAAGGTGAAGAAAATCCAACTCCTGAAGAAAAATTAATGGCTGCAATTTTTGGTCAAAAAACTGCCTCAAGAAAAGATACTTCTCTAAAAGTAAAACATGGCCACAATGGAACCGTTGTTGCTGTTGATATTCTAAGTAGAGAATTTGGCGACCAATTAGAAGATGGCATTGAAAAAATAGTTAAGGTATCAATTGCTCAAAAACGTAAAATTCAAGTCGGCGACAAGATGGCTGGTCGTCACGGAAATAAAGGTGTTGTTTCAATAGTATTGCCTGTTGAAGAAATGCCTTATTTAGCAGATGGAACACCATTAGATATTGTTTTAAACCCTCAAGGTGTCCCTTCACGTATGAACATTGGTCAAGTTCTAGAATTGCACTTAGGACTTGCTGCTAAAAAATTAAATACAAAATTTGTTACACCAATATTTGATGGAATTTCACACAACCAAATTCGTGATATTCTTGAAGAAGCAAATATTGATGTAACAGGAAAATCAATAGTATACGATGGGGCCACTGGAGAACCATTTGATCACCCAATTTCTGTAGGAATTATGTACTATTTGAAGTTGTATCACATGGTTGATGACAAAATGCACGCACGTTCAGTAGGACCTTATTCATTGATTACCCAACAACCACTTGGTGGAAAGAGTCAAAATGGTGGTCAAAGATTTGGTGAAATGGAAACATGGGCTATTGAATCATATGGAGCTTCAAATGTTCTACAAGAATTATTAACATATAAATCTGATGATATTGTTGGACGTAATAAACTATATAACTCTCTTGCTAGAGATGCAAGATTGCCTAAACCAGGAACACCTGAATCATTTAATGTTTTAGCATATGAATTACGTGGTTTAGGAATTAAATTAGAAGTACATCAAAAATCTGATAATGTCGTAGATGATTCAGAAGAAGATCAAAGAGTAGACACAAAATATTTCCAAGAATTTGAAGGGAGTTTAGAATAA", "aa": "MVKDDVYTSIHIEEQSIQFRHSKAGEDILTADIPNVSNYSKRFLGENGIVRVGSEVTAGDILVGRTSPKGEENPTPEEKLMAAIFGQKTASRKDTSLKVKHGHNGTVVAVDILSREFGDQLEDGIEKIVKVSIAQKRKIQVGDKMAGRHGNKGVVSIVLPVEEMPYLADGTPLDIVLNPQGVPSRMNIGQVLELHLGLAAKKLNTKFVTPIFDGISHNQIRDILEEANIDVTGKSIVYDGATGEPFDHPISVGIMYYLKLYHMVDDKMHARSVGPYSLITQQPLGGKSQNGGQRFGEMETWAIESYGASNVLQELLTYKSDDIVGRNKLYNSLARDARLPKPGTPESFNVLAYELRGLGIKLEVHQKSDNVVDDSEEDQRVDTKYFQEFEGSLE", "aa_hexdigest": "64194ddd54b985f768b118196d0dc5de", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030B82D9C", "query_cov": 0.9873096446700508, "subject_cov": 0.8644444444444445, "identity": 0.715, "score": 555.0, "evalue": 5.4e-195, "valid": false, "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_A0A352BHB6", "kegg_orthology_id": "K03043" }, "pscc": { "uniref50_id": "UniRef50_A0A352BHB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352BHB6" ], "product": "DNA-directed RNA polymerase" }, "genes": [], "id": "DFOKDJOJIG_663", "locus": "GCA013367855_00647" }, { "type": "cds", "sequence": "contig_1", "start": 366256, "stop": 367461, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04565.21", "PFAM:PF10385.14", "PFAM:PF00562.33" ], "nt": "ATGTTGCATGACCAAAAAGTTTTGGTTTTAGGTAATGATCCTACATCAACAGAAAATTTCTTGTTGATTCCTGATTTAATTGCAACGATTTCGTATTATTTTAATTTACTTTCAAAGGTTGGGGTTGATGATGATCCTGACTCATTAGTTAATAAAAGAATTGTAACTATAGGCGAATTATTACAAAATCAATTTAGAATAGGGCTATTAAAATTAGAAAAAAATACAAGAGAAAGAATCTCGGCAAAAGATATTGATAAGATTACTGCTAAGAACGTTACAAACAATAAACCAATATTTAATCAATTTAAATCGTTCTTTAACACATCAAAACTTTCTCAATTTATGGATCAATGTAATCCTCTTGCTGAAATATCAAATAAGAGAAGAATAACCTCCCTAGGACCAAGAGGGTTGAACCGTGATACTGCACAATTTGAAGTTCGTGACGTTCACCCTACCCATTATGGAAGAATTTGTCCTATTGAAACACCCGAAGGACCAAACATTGGTTTAATTTTAAATTTAGCATCATTTGCTAAAATTGATAAATATGGTTTTATTCAATCACCATATTTCAGAGTAAATAAAAAAGTTGTAGATTTTTCAGAACCAGTTTACATTACTGCAATTGAAGAAACAGGATATACATTTGCACAATCAACTGTTCACATTGAAGGTAATGTAATTGTTGATGAAAAAGTAATGGTAAGACGTGACAATGAATATTTAGAAGTAAATGCTGAAGATGTAGATTTTATTGGTGTCTCAAACCGTCAAATGACCTCAATAGCTGCTTCGGCTATACCATTTTTGGAAAACGATGATGCTAACCGTGCATTGATGGGTTCTAACATGCAACGTCAAGCTGTTCCAGTGTTATTCCCAGAAGCTCCATTAGTTGCTACAGGAGTTGAAGCTGATATTGCTAAATATTCATCAACCAATATTAGAGCAACCAGATCAGGGGTTGTTAACTATGTAGATGCTAACGTTATTAAAGTAACTCCTGAAAATAGTTCAAAAAGTGATGTTTATCATTTAAGAACATTTGAAAGAAGTAACCAAGGTACATTAATTCACCAAGTACCTTTGGTTAAAGTTGGCCAACAAATTAATGCAGGTGATTTATTAGTTGATGGTCCTTCAATGAAAGATGGAGAATTAGCATTAGGAAAAAATGTTCTAGTAGGTTTTACTACTTGA", "aa": "MLHDQKVLVLGNDPTSTENFLLIPDLIATISYYFNLLSKVGVDDDPDSLVNKRIVTIGELLQNQFRIGLLKLEKNTRERISAKDIDKITAKNVTNNKPIFNQFKSFFNTSKLSQFMDQCNPLAEISNKRRITSLGPRGLNRDTAQFEVRDVHPTHYGRICPIETPEGPNIGLILNLASFAKIDKYGFIQSPYFRVNKKVVDFSEPVYITAIEETGYTFAQSTVHIEGNVIVDEKVMVRRDNEYLEVNAEDVDFIGVSNRQMTSIAASAIPFLENDDANRALMGSNMQRQAVPVLFPEAPLVATGVEADIAKYSSTNIRATRSGVVNYVDANVIKVTPENSSKSDVYHLRTFERSNQGTLIHQVPLVKVGQQINAGDLLVDGPSMKDGELALGKNVLVGFTT", "aa_hexdigest": "a5684565194a5de4b4ea6da7d1130d75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04565.21", "name": "RNA_pol_Rpb2_3", "length": 69, "aa_cov": 0.17206982543640897, "hmm_cov": 0.9855072463768116, "evalue": 8.173182683589838e-30, "score": 98.50003814697266, "start": 114, "stop": 182 }, { "id": "PF10385.14", "name": "RNA_pol_Rpb2_45", "length": 67, "aa_cov": 0.16458852867830423, "hmm_cov": 0.9850746268656716, "evalue": 2.4683261318992543e-15, "score": 51.9917106628418, "start": 192, "stop": 257 }, { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 86, "aa_cov": 0.20448877805486285, "hmm_cov": 0.8837209302325582, "evalue": 2.1221504128360184e-10, "score": 35.854862213134766, "start": 319, "stop": 400 } ], "seq_stats": { "molecular_weight": 44613.200400000074, "isoelectric_point": 5.792484474182129 }, "id": "DFOKDJOJIG_664", "locus": "GCA013367855_00648" }, { "type": "cds", "sequence": "contig_1", "start": 367633, "stop": 367959, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04561.19" ], "nt": "ATGTTTGGCGAAAACATTCCAGAATTAGAAGAAACATTTAAACGTGATAAAATCACTGGAATTAATGCAGAAGAAGTTACAAGGGAAGCACAAGAAGCAATTTATAGAATCATTAGAAAAGGCGATCGTATGACAGCTGATTCTGCTAGAAATTTAATTCCTACAACTTTGTTTAATGAAAAAAGATATAGTTTAACTGAAACAGGACGTTTCACTTTAAATAGAAAATTAAACATTATTGAAAGAATTGCTAATTCTTATTTAGCAGAAGATATTTTTTCTAGTGAAGGTGAACTTCTTTATGAAAAAGGAATGTTCATTACTTGA", "aa": "MFGENIPELEETFKRDKITGINAEEVTREAQEAIYRIIRKGDRMTADSARNLIPTTLFNEKRYSLTETGRFTLNRKLNIIERIANSYLAEDIFSSEGELLYEKGMFIT", "aa_hexdigest": "381928d4fce804669575b1b026c59d28", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04561.19", "name": "RNA_pol_Rpb2_2", "length": 71, "aa_cov": 0.6296296296296297, "hmm_cov": 0.9154929577464789, "evalue": 3.201694127355486e-06, "score": 22.57516098022461, "start": 27, "stop": 94 } ], "seq_stats": { "molecular_weight": 12558.086299999986, "isoelectric_point": 5.114622688293457 }, "id": "DFOKDJOJIG_665", "locus": "GCA013367855_00649" }, { "type": "cds", "sequence": "contig_1", "start": 368089, "stop": 368628, "strand": "-", "frame": 1, "gene": null, "product": "RNA polymerase beta subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFDE54", "UniRef:UniRef90_UPI002FDFDE54" ], "nt": "ATGGTGAATAAGGATAAATACGAAATTAGAAAATTTGGACCTATTACCCTAAGACGTGACTATTCAATTAGTCAAAAGAAATTCGAAACTCCTGACTTTCTTGAAATGCAACGTGAATCAGTTGAAAAATTTCTAACAGTTGGAATTGAAGAAGAATTAAGAAATATCTATCCAATTGAAGCACATGGAAAAGTTAGAATTGAATACATTCATAATTCAGCACATTTTGAATATCCTAAAAAAACTGAATATGAAAGCATTAAAGAAGCTAAACAAAAAGGTTCATCATATCAAGGAAAGTTAAAAGCACACCTAAGACAAATCAATATCGAAACAGGTGAAGTTGAAGATTCAGAAGTAGTATTTGCTGAAATTCCTATAATGACTTATGGTGGATCATTTATTATCAATGGTTCAGAAAAAGTTATTGTTTCGCAATTAATTAGATCTACCGGGGCTTATTTTGGAATTAATGTTAGAAATAAACAAGCCAACGACTTGTTTAATAAAGTAGAAATTATTCCTCAAATTGGTTCATGA", "aa": "MVNKDKYEIRKFGPITLRRDYSISQKKFETPDFLEMQRESVEKFLTVGIEEELRNIYPIEAHGKVRIEYIHNSAHFEYPKKTEYESIKEAKQKGSSYQGKLKAHLRQINIETGEVEDSEVVFAEIPIMTYGGSFIINGSEKVIVSQLIRSTGAYFGINVRNKQANDLFNKVEIIPQIGS", "aa_hexdigest": "73e8e121e79577765c555dbc7556d4bc", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 367786, "stop": 368619, "observations": { "mutation": [ 368091 ], "stop": [ 368091 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FDFDE54", "query_cov": 0.9832402234636871, "subject_cov": 0.5482866043613707, "identity": 0.8859999999999999, "score": 307.0, "evalue": 4.96e-103, "gene_start": 4, "gene_end": 179, "reference_start": 3, "reference_end": 178, "reference_sequence": "MVKAKYELRKFGPITERRDYSISQKKFATPDFLEMQRTSVENFIHQGIEEELRNIYPIESHGKVRIEYVWNSAHFDYPKKSEFESIKEAKQKGSSYQGKLKARLRQINLETGEVEESEVVFAEIPIMTYGGSFIINGSEKVIVSQLIRSTGAYFGLNVRNKQANDLFNKVEIIPQVGSWVEIYHKVTSANADSVKIHIDKNKSFLLTTFLKALGFSETGIRAMFGDIAELEETLKKDKIVGLSLEESTREAQEAIYRIIRKGDRMTVESARNLIPSTLFNEKRYNLTQIGRFTLNRKLNVIERIANSFLAESIFSDKGELL" }, "paralog": false, "identity": 0.851985559566787, "score": 465.0, "evalue": 1.15e-167, "description": "Internal stop codon at 368091. Nonsense mutation around 368091.", "psc": { "uniref90_id": "UniRef90_UPI002FDFDE54", "product": "RNA polymerase beta subunit", "uniref50_id": "UniRef50_UPI002FDFDE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FDFDE54", "UniRef:UniRef50_UPI002FDFDE54" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FDFDE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFDE54" ] } }, "id": "DFOKDJOJIG_666", "locus": "GCA013367855_00650" }, { "type": "cds", "sequence": "contig_1", "start": 368762, "stop": 369136, "strand": "-", "frame": 3, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_012855544.1", "SO:0001217", "UniParc:UPI00000BF3D8", "UniRef:UniRef100_D1J856", "UniRef:UniRef50_B3PM75", "UniRef:UniRef90_B3PM75" ], "nt": "ATGGCAAAATTAACAAAAGAAGAATTTGTTTCAGCATTAAAAGAAATGAACATTAAAGAAGTTATGGAATTAATTCAAGGATTAAAAGAAGAATTTGGAATTGATCCAACAGCAGTTGTAGCAGCAGCAGCTCCAGCAGCAGCAGCTGACGCAGCAGAAGAAAAAACTGTATTTAATGTTACTTTAAAATCAGATGGTGGAAACAAGCTAGCAGTTATTAAAGTTGTTAAAGACTTATTAGGTCTTGGATTAATGGATGCTAAAAAACTTGTTGAATCAGCACCTGTTGTAATTAAAGAAAATGCTAAAAAAGAAGAAGCTGAAGAACTAAAAGCTAAATTAACAGAAGCTAAAGCCGAAGTTACTTTAGACTAA", "aa": "MAKLTKEEFVSALKEMNIKEVMELIQGLKEEFGIDPTAVVAAAAPAAAADAAEEKTVFNVTLKSDGGNKLAVIKVVKDLLGLGLMDAKKLVESAPVVIKENAKKEEAEELKAKLTEAKAEVTLD", "aa_hexdigest": "53b91ce3f730c59f29e8ab79a1e81e35", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000BF3D8", "ncbi_nrp_id": "WP_012855544.1", "uniref100_id": "UniRef100_D1J856", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BF3D8", "RefSeq:WP_012855544.1", "UniRef:UniRef100_D1J856" ] }, "ips": { "uniref100_id": "UniRef100_D1J856", "uniref90_id": "UniRef90_B3PM75" }, "psc": { "uniref90_id": "UniRef90_B3PM75", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_B3PM75", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM75", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM75" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "DFOKDJOJIG_667", "locus": "GCA013367855_00651" }, { "type": "cds", "sequence": "contig_1", "start": 369159, "stop": 369656, "strand": "-", "frame": 2, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02864", "RefSeq:WP_012855545.1", "SO:0001217", "UniParc:UPI0001BC223D", "UniRef:UniRef100_D1J857", "UniRef:UniRef50_A0A448ZYM3", "UniRef:UniRef90_D1J857" ], "nt": "ATGAGCGCATTAAAAGATGCCAAGGTTTTAGTTGTTGAAGAAATTTCAAACAATTTAAAAGAATCACAAGCATTATATGTAATTAATTATGCTACTTTGGATGTTGTTTCATTTCAAGAAATTAGAAAAGAATTAGCTCCAAATAATGCATTGCTAAAAGTTTACAAAAATAGACTAGTTAAACAAGCATTAAAAAATACAGAATATGCAAATATTAATGATAGTTTAGTTTTACAAAATGCTTATGCCTTTGTTAAAGGCGATTCATTAGCAGCATTAAAAACCTTAGTAGGCTTAAAGAAAAAATTCCCTGCATTAAGAATTGTAAGTGGAATTTATGAAAATAAAGTAGTTGATGAAAAAACATTAGACGAAATATCAAAACTTCCTTCATTTACAGAATCACTTATGATTCTTGGAAATTCATTATTGAGTCCATTGAAACAATTATCAATTGGTTTAAATGAATTAATAAAACAAGGAAAAATTTCAGAATAA", "aa": "MSALKDAKVLVVEEISNNLKESQALYVINYATLDVVSFQEIRKELAPNNALLKVYKNRLVKQALKNTEYANINDSLVLQNAYAFVKGDSLAALKTLVGLKKKFPALRIVSGIYENKVVDEKTLDEISKLPSFTESLMILGNSLLSPLKQLSIGLNELIKQGKISE", "aa_hexdigest": "4e952e6b06822b7a4ca63497eef65389", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC223D", "ncbi_nrp_id": "WP_012855545.1", "uniref100_id": "UniRef100_D1J857", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC223D", "RefSeq:WP_012855545.1", "UniRef:UniRef100_D1J857" ] }, "ips": { "uniref100_id": "UniRef100_D1J857", "uniref90_id": "UniRef90_D1J857" }, "psc": { "uniref90_id": "UniRef90_D1J857", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_A0A448ZYM3", "cog_id": "COG0244", "cog_category": "J", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYM3" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "DFOKDJOJIG_668", "locus": "GCA013367855_00652" }, { "type": "rRNA", "sequence": "contig_1", "start": 369877, "stop": 372772, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9900854700854701, "score": 2767.4, "evalue": 5e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TATTAAACAAGCAAGAGTTTTTGGTGGATGCCTTGGGTCTGGAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGGTTAGCTGGAAATAAGCTGTTATCCGGGGATTTCCGAATGGGGAAACCTAATTGAGCTAATCCTCAATTATCATATCGATGAATTCATAGTCGAATGAAGAGACACGCTGTGAATTGAAACATCTCAGTAGCAGCAGGAAGAGAAAATAAAGAATGATTCCCTAAGTAGTGGCGAGCGAACGGGGAAGAGCCCAAACCAACACATGTTGTTGGGGTTGTAGGACTGCGACATGGATTAATGAATTTGTACATAGCAGAATAAGTTGGAACGCTTAAACATAGAGGGTGAAATTCCCGTAAGCGAAATGTATAAATCTCCTAGCAGTATCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATTTGCCGGGACCACCCGGTAAGGCTAAATACTAACCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGGGAGTGAAATAGATTCTGAAACCAATTACTTACAGTTAGTCAGAGCCCGTTAATGGGTGATGGCGTACATCTTGCAGAATGGACCGGCGAGTTATGTCAACATGCGAGGTTAAGTAGAATAAAGCGAAGCCGTAGAGAAATCGAGTCTGAATAGGGCGATTTAGTATGTTGATATAGACCCGAAACCAGGTGATCTACCCATGAGCAGGTTGAAACTTAGGTAACACTAAGTGGAGGACCGAACCGCAGTACGCTAAAAAGTGCCCGGATGACTTGTGGGTAGGGGTGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAATGATGGGGGTAGAGCACTGAATATGGAATGGCGGCGCCTAGCCGTACTGACTATAATCAAACTCCGAATACTATCATGTATTACTATGCAGTCGGTACATCGGTGATAACGTCGATGCACGCGAGGGGAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGTGTTAAGTGAGAAAGGTTGTGAAGTTTCTTAAACAGCTAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAATAGCTCACTAGTCGAGAGACTTTGTGCCGATAATTCAACGGGACTAAAACACAATACCGAAGCTACGGGCAGAAATGCGTTAGGAGAGCGTTGTAAGGGCTTAGAAGCCAGACCGTGAGGACTGGTGGAGCGCTTACAAGTGAGAATGCCGGTATGAGTAACGATTCAGAGTGAGAATCTCTGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCGGGTCCTAAGACGAGGCCGAAAGGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTTAACATGTGATGGAGTGACGGGGAAGGATAATTTTACCACTAATTGGATTGTGGGGTAAGTAACAACTGGGTTATGTAGGCAAATCCGCATAACTTAACTGGGAGTTACGATGCATAGCAAAAGGGCAACTGAGTAGCGAATTAGATGATTTCATGCCTCTTAAAAAAGCTTCTAACATTAAGTGTTAGGAAACCCGTACCGAGAACGGACACACGTCCCCAAGATGAGTATTCTAAGGCGAGCGAGAAAACTATTGTCAAGGAACTCTGCAAATTCATCCCGTAAGTTCGCAAGAAGGGATGCCCACAATAAAATGTGGGCCGCAGTGAATAGTAAGGGGGAACTGTTTATCAAAAACACAGCTCTATGCTAAGTCGTAAGATGATGTATATGGGGTGACTCCTGCCCAGTGCCCGAAGGTTAAGCAGAGGTGTTAGCATTAGCGAAGCATTAATGTGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGAGTAATTATCTCTTAACTGTCTCGACAATAGACTCGGTGAAATTATGGTTCCGGCGAAGACGCCGGAGACCCGCATCTAGACGAAAAGACCCCGTGGAGCTTTACTATAACTTCATATTGGAGTTTGATTTAACATGTGTAGGATAGGTGGGAGACGTTGATGCTTGAACGCTAGTTTAAGAGTAGTCGCCGTTGAAATACCACCCTTGTTACATTGAACTTCTAACTTGTTCCCGTCATCCGGGAAGAGGACAGTGTGTGGTGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGGGTAACGGAGGCGTTCAAAGTTACACTCAATACGGTCAGAAACCGTATCTAAGAGCATAAAGGTAGAAGTGTGATTGACTGTGAGACCTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCGGTTCTTCGTGGAAAGGCCGTCGCTCAACGGATAAAAGCTACCCCGGGGATAACAGGCTTATCTTTCCCAAGAGATCACATCGACGGGAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTTCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCTACTCTTAGTACGAGAGGACCGGAGTGGACGCACCGATGGTGTGCCAGTTGTTTCGCCAGAAGCATAGCTGGGTAGCCAAGTGCGGCAGGGATAACCGCTGAAAGCATCTAAGCGGGAAGCCCCCTCAAAGATTAGTATTCCCTTTAAAATTCCTTATAGACTATGAGGTTGATAGGCTGGAGGTGTAAGTGCAGCAATGCATTCAGCTGACCAGTACTAATAAATTTATTGGTTTAATAGT", "id": "DFOKDJOJIG_39", "locus": "GCA013367855_00653" }, { "type": "rRNA", "sequence": "contig_1", "start": 372956, "stop": 374473, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1513.4, "evalue": 2.6e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTATAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAGGTTAGCAATAACCTAGCGGCGAATGGGTGAGTAACACGTGCTTAATCTACCTTTTAGATTGGAATACCCATTGGAAACAATGGCTAATGCCGGATACGCATGGAACCGCATGGTTCCGTTGTGAAAGGCGCTGTAAGGCGCCACTAAAAGATGAGGGTGCGGAACATTAGTTAGTTGGTGAGGTAATGGCCCACCAAGACTATGATGTTTAGCCGGGTCGAGAGACTGAACGGCCACATTGGGACTGAGATACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCACGATGAAGGTCTTCGGATTGTAAAGTGCTGTTATAAGGGAAGAACATTTGCAATAGGAAATGATTGCAGACTGACGGTACCTTGTCAGAAAGCGATGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTTCGTAGGCTGTTTGTTAAGTCTGGAGTTAAATCCCGGGGCTCAACCCCGGCTCGCTTTGGATACTAGCAAACTAGAGTTAGATAGAGGTAAGCGGAATTCCATGTGAAGCGGTGAAATGCGTAGATATATGGAAGAACACCAAAGGCGAAGGCAGCTTACTGGGTCTATACTGACGCTGAGGGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTCGGTGGAGAATCACTGACGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACACGGAAAACCTTACCCACTCTTGACATCCTTCGCAAAGCTATAGAGATATAGTGGAGGTTATCGGAGTGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTGCAACGAGCGCAACCCCTATCTTTAGTTACTAACATTAAGTTGAGGACTCTAGAGATACTGCCTGGGTAACTGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCCACACACGTGCTACAATGGTCGGTACAAAGAGAAGCAATATGGCGACATGGAGCAAATCTCAAAAAGCCGATCTCAGTTCGGATTGGAGTCTGCAATTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCTATGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATACCCAAAGTCGGTTTGCTAACCTCGGAGGCGACCGCCTAAGGTAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "DFOKDJOJIG_35", "locus": "GCA013367855_00654" }, { "type": "cds", "sequence": "contig_1", "start": 374859, "stop": 375056, "strand": "-", "frame": 2, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "COG:COG0333", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_012855546.1", "SO:0001217", "UniParc:UPI0000DB8019", "UniRef:UniRef100_D1J858", "UniRef:UniRef50_Q4A6N8", "UniRef:UniRef90_D1J858" ], "nt": "ATGGCAGTAGTACCAAAAAGAAAAACTTCAAAACAAAGAAAACATTTAAGAAGATCACACCACGCTCTAGTGGCTCCAACATTAGTTGAATGTTCACAATGTAAGAATTTAATTACTCCTCACCAAGCATGTGAAAACTGCGGTTTTTATAGAGGAAGAAAAGTTATTAAAGAAGCAATTAACGATAAAATTAAATAA", "aa": "MAVVPKRKTSKQRKHLRRSHHALVAPTLVECSQCKNLITPHQACENCGFYRGRKVIKEAINDKIK", "aa_hexdigest": "2966d01971762bee811933c45cb0dcc6", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000DB8019", "ncbi_nrp_id": "WP_012855546.1", "uniref100_id": "UniRef100_D1J858", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000DB8019", "RefSeq:WP_012855546.1", "UniRef:UniRef100_D1J858" ] }, "ips": { "uniref100_id": "UniRef100_D1J858", "uniref90_id": "UniRef90_D1J858" }, "psc": { "uniref90_id": "UniRef90_D1J858", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_Q4A6N8", "cog_id": "COG0333", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N8" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "DFOKDJOJIG_669", "locus": "GCA013367855_00655" }, { "type": "cds", "sequence": "contig_1", "start": 375081, "stop": 375368, "strand": "-", "frame": 2, "gene": null, "product": "Large ribosomal subunit protein bL17", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20277" ], "nt": "TTGATTAATGGCAAAGTAACAACCACATTAGAACGTGCTAAAAGAATTCGTTCAAATGCAGAAAAAATGATTACTCTAGGTAAGAAAAATACCCTAGCTTCGCGTAGACAAGCAGCCAAATATTTAAGATTAATTGCAACAGAAAACAAAAATAAAAATTCATTACAATATTTATTTGATGTTGTGGCACCAAAATACGTTGAACGTAATGGTGGATATACAAGAATTACAAAATTAGCCAATCGTGCTGGTGATAATGCTAAAATGGCAATAATCGAATTGGTATAA", "aa": "MINGKVTTTLERAKRIRSNAEKMITLGKKNTLASRRQAAKYLRLIATENKNKNSLQYLFDVVAPKYVERNGGYTRITKLANRAGDNAKMAIIELV", "aa_hexdigest": "624475da950116ad9799080f8a5457a3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FA24CA4", "query_cov": 1.063157894736842, "subject_cov": 0.808, "identity": 0.564, "score": 107.0, "evalue": 2.53e-28, "valid": false, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P20277", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P20277", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20277" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [], "id": "DFOKDJOJIG_670", "locus": "GCA013367855_00656" }, { "type": "cds", "sequence": "contig_1", "start": 375515, "stop": 376483, "strand": "-", "frame": 3, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_P38018", "UniRef:UniRef90_D1J860" ], "nt": "ATGAAGAAAATACAAAAAATTACATATAAAGAATTAGTAGCAGAAAAAGTATCAGATTTTAATACAACATTTGTTATTGAACCACTAATGCGTGGTTATGGTAACACAATAGGAACTGTAATAAGAAGAACATTACTTTCTTCAATTACTTCTGTTGCTCCATTTGCAATAAAAATTAGTCATGTTGAACATGAATTTACTGCAATTGAAGGATTAAGAGAAGATGCTATTACATTAGTTGCAAACATAAGAAAAATTCGCTTTGCATATGACCCAGAATTATTTGAAAAAGATAATTTAGCTAAAATTTCATTTAAATCAACAAAAGAAGGGGAAGTTCATGCTTCGGATATTGAAACTATCCCTGGACTTGACATTGTTAATAAAGATCAATACATAGCAACAATATCAAAAAATGGTTCATTGGAATTTGAATTATATTTAAGAACCGGTAGAGGCTATGTTTCATTTGAAGACAATAAAAAAACCATTCTAGAATATGGTCCAAAATTAGAATCAAAAATCAAAAATGGTCAATTTTTGGCAATGGATAGTGACTTCAGTCCTATTAAAAAAGTTGCGATTTTATTTGAAGAATTAAATTCTACTTCTAACATAATTGAAGAAAGATTAAAAATTAAAGTTGAAACCGATGGAACTGTTGAAGCTAAAAACGTTATGGAAGAAGCAGCAAAGATTATAGTTGCTCATTTCCAAATCATTGGAAACATTGACGCATTAGGAACAATTGATTTATTCGATGATCAAAAAGAAAAACATGAAAAAACTCCAAAAGTTTCAGTTTCTATTGATAAATTGAATTTGACAATTCGTTCATTGAATGCCTTAAGACGTGCTGGATTTAATAATGTTGATGAAATAATGAAATTAAGCGATGAAGAACTTTCGAATATTAAAAATTTGGGTAAAAAATCAGTACAAGATATTATTGACCGTCGTAGAGAATGA", "aa": "MKKIQKITYKELVAEKVSDFNTTFVIEPLMRGYGNTIGTVIRRTLLSSITSVAPFAIKISHVEHEFTAIEGLREDAITLVANIRKIRFAYDPELFEKDNLAKISFKSTKEGEVHASDIETIPGLDIVNKDQYIATISKNGSLEFELYLRTGRGYVSFEDNKKTILEYGPKLESKIKNGQFLAMDSDFSPIKKVAILFEELNSTSNIIEERLKIKVETDGTVEAKNVMEEAAKIIVAHFQIIGNIDALGTIDLFDDQKEKHEKTPKVSVSIDKLNLTIRSLNALRRAGFNNVDEIMKLSDEELSNIKNLGKKSVQDIIDRRRE", "aa_hexdigest": "87e58c6c79dce2574865c9b0167ca5f9", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J860", "query_cov": 1.0, "subject_cov": 0.936046511627907, "identity": 0.997, "score": 608.0, "evalue": 4.63e-219, "valid": true, "gene": "RpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P38018", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P38018", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38018" ], "product": "DNA-directed RNA polymerase subunit alpha" }, "genes": [ "rpoA" ], "id": "DFOKDJOJIG_671", "locus": "GCA013367855_00657" }, { "type": "cds", "sequence": "contig_1", "start": 376777, "stop": 376887, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAGAAAAATAAAAAAGTTATTACTCAAGGTATTGCACATATTCACTCAACTTACCAAAACACAATTGTTTCTTTCTCAGATCTAAAAGGAAATGTTTTTGCTTGA", "aa": "MAKKNKKVITQGIAHIHSTYQNTIVSFSDLKGNVFA", "aa_hexdigest": "a34c7eb380a59aa586242df175c0d8bb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3990.5867000000007, "isoelectric_point": 10.000351905822754 }, "id": "DFOKDJOJIG_672", "locus": "GCA013367855_00658" }, { "type": "cds", "sequence": "contig_1", "start": 376890, "stop": 377258, "strand": "-", "frame": 2, "gene": "rpsM", "product": "30S ribosomal protein S13", "db_xrefs": [ "COG:COG0099", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02952", "RefSeq:WP_012855550.1", "SO:0001217", "UniParc:UPI0001BC2240", "UniRef:UniRef100_D1J862", "UniRef:UniRef50_P38017", "UniRef:UniRef90_D1J862" ], "nt": "ATGGCCAGAGTTTTAAACATTGAAATTCCTAATAATAAAAAAGTTCGTATTTCACTTACTTACATCTTTGGTATTGGCGTTTCACTAGCAAAAGAAATTTTAAAAGATGCTAATGTTGACGGCGAAAAAAGAGTTAAAGATCTTAGCGAAGAAGAATTAACTAGAATCAGAGATGAAGCTAAAAAATACACCACTGAAGGTGATTTAAGAAGAGAAATAAATTTAAACATTAAAAGATTAATGGAAATTAAATCATATCGTGGTATTAGACACCGTAAGGGATTACCTGTACGTGGTCAATCTACAAAGAAAAATGCAAGAACAAGAAAAGGTCCAAGAAAAACAATAGCAGGAAAGAAAGGTAAATAA", "aa": "MARVLNIEIPNNKKVRISLTYIFGIGVSLAKEILKDANVDGEKRVKDLSEEELTRIRDEAKKYTTEGDLRREINLNIKRLMEIKSYRGIRHRKGLPVRGQSTKKNARTRKGPRKTIAGKKGK", "aa_hexdigest": "ac04cc895c8f114982ffe618d49356fa", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2240", "ncbi_nrp_id": "WP_012855550.1", "uniref100_id": "UniRef100_D1J862", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2240", "RefSeq:WP_012855550.1", "UniRef:UniRef100_D1J862" ] }, "ips": { "uniref100_id": "UniRef100_D1J862", "uniref90_id": "UniRef90_D1J862" }, "psc": { "uniref90_id": "UniRef90_D1J862", "gene": "rpsM", "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_P38017", "cog_id": "COG0099", "cog_category": "J", "kegg_orthology_id": "K02952", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P38017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38017" ], "product": "Small ribosomal subunit protein uS13" }, "genes": [ "rpsM" ], "id": "DFOKDJOJIG_673", "locus": "GCA013367855_00659" }, { "type": "cds", "sequence": "contig_1", "start": 377387, "stop": 377602, "strand": "-", "frame": 3, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0006413", "KEGG:K02518", "RefSeq:WP_012855552.1", "SO:0001217", "UniParc:UPI0001BC2242", "UniRef:UniRef100_D1J864", "UniRef:UniRef50_Q4A5I6", "UniRef:UniRef90_D1J864" ], "nt": "ATGGCAAAAGACGCAATCAAAATGTCAGGCAAAATTACTAAAATGCACACATCAAGAGACTATGATGTTTTATTAGAAAATGGACTTACTATTAAAGCTTCTATTTCAGGTAAAATGTCACTTCACAATATCAAAATGATCCCTGGGGATTTAGTTGATGTTGAACTTAGTCCATATGATATGAGCAGAGGTAGAATTGTTTTTAGACACAAATAA", "aa": "MAKDAIKMSGKITKMHTSRDYDVLLENGLTIKASISGKMSLHNIKMIPGDLVDVELSPYDMSRGRIVFRHK", "aa_hexdigest": "00b621bb30ccf8e19c9059b63d700fad", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2242", "ncbi_nrp_id": "WP_012855552.1", "uniref100_id": "UniRef100_D1J864", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2242", "RefSeq:WP_012855552.1", "UniRef:UniRef100_D1J864" ] }, "ips": { "uniref100_id": "UniRef100_D1J864", "uniref90_id": "UniRef90_D1J864" }, "psc": { "uniref90_id": "UniRef90_D1J864", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q4A5I6", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5I6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5I6" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "DFOKDJOJIG_674", "locus": "GCA013367855_00660" }, { "type": "cds", "sequence": "contig_1", "start": 377701, "stop": 378354, "strand": "-", "frame": 1, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0006508", "GO:0070006", "SO:0001217", "UniRef:UniRef50_L0RW44", "UniRef:UniRef90_D1J865" ], "nt": "ATGGTCATAATAAAAAATCAATTTGAAATTGAAAAGATCAAAAAAGCTTGTAAGATCCTGGCAGAAGTGAAAACTGTTTTATACGACTTTATAAGACCAGGCGTTTCTTTAAAAGAAATTGATAGCGTAGCTTTTAAAGAAATAAGAAAAAGGGGTGGAAAACCAGCATTCTTAGGACAATATGGTTTCCCTAATACGTGTTGCATTTCTGTTAATAATGAATTAATTCACGGAATTCCTAGTGATTATGTTGTAAAGGATGGAGATATAGTCAAAATTGACACTGGAGCAATTTATGATGGCTATTATTCTGATTCAGCATTTACAAAAGGAGTTGGCAACATAACTGAAGCAGACAAAAAATTAATTGCCTGCGCAAAAGACGCTTTTTATGCCGGCTTTAATGCAATTAAAGTTGGCAAACACATTGGTGATATTTCTGCAGCCATAGGAAATTTAATTCGTGATAGAGGATATTTTACTCCAGATGAATATACAGGTCACGGAATAGGCAGAAAGTTGCATGAAGATCCATATGTTTATAACGATGGCTATCCAGGAACTGGACAGCTAATTAGAAATGGTATGGTAATTTGCATTGAACCAATGATTTTACAAAAATCTAAAAATGTATTGGTCAAAGGAAATGGATGA", "aa": "MVIIKNQFEIEKIKKACKILAEVKTVLYDFIRPGVSLKEIDSVAFKEIRKRGGKPAFLGQYGFPNTCCISVNNELIHGIPSDYVVKDGDIVKIDTGAIYDGYYSDSAFTKGVGNITEADKKLIACAKDAFYAGFNAIKVGKHIGDISAAIGNLIRDRGYFTPDEYTGHGIGRKLHEDPYVYNDGYPGTGQLIRNGMVICIEPMILQKSKNVLVKGNG", "aa_hexdigest": "f0771c603bdc7f9bf4e85fd5e65ecb54", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_D1J865", "query_cov": 1.0, "subject_cov": 0.868, "identity": 1.0, "score": 442.0, "evalue": 1.02e-156, "valid": true, "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_L0RW44", "cog_id": "COG0024", "cog_category": "J", "go_ids": [ "GO:0006508", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW44" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "DFOKDJOJIG_675", "locus": "GCA013367855_00661" }, { "type": "cds", "sequence": "contig_1", "start": 378345, "stop": 379040, "strand": "-", "frame": 2, "gene": "adk", "product": "adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0005524", "GO:0006139", "GO:0019205", "RefSeq:WP_020002415.1", "SO:0001217", "UniParc:UPI000376F4E5", "UniRef:UniRef100_UPI000376F4E5", "UniRef:UniRef50_Q6KI35", "UniRef:UniRef90_D1J866" ], "nt": "ATGATAGAAAAATCTCAAGAAGTGTACAACGAACACGCAAAAAAATTTCCTAATATAATTATGCTTGGCGCACCAGGAGCTGGTAAAGGCTCTATTGCAACAAAAATGGTGCAAAATAATGATTATGTTCAGATTTCAACTGGAGATATGTTTAGACAAGAAATTAAAAATCAAACTCCCCTTGGCTTAAAAATAAAATCTATTTTAGATTCGGGAGCATATGTTGATGATTGTATAACAAATCAACTAGTTGAAAAAAAATTAACTGAATTAGTAAGAGAAAATAAACACTTCATATTAGACGGATATCCCCGTACATTAGCTCAAGCAAAGTTTTTACTTTCATTAGCAAATGTTGGAATCAAAATTGATAAAGTAATTTTACTAGAAATTACTGATAAACAAATTATTGACCGTCTTTCAAAACGTAGAATTTGTTTAAATTGTAAAACCATTTATCATTTGCAATCATTTCCACCATTACCTGGTGATTTGTGTATTAAATGCCACGAAAAAGTAACAAAAAGACCAGACGACGAACCAGAAGTAATAAAGAAACGTTTATCAATATATAATGAACAAACTAAATGTTTGATCAATTTTTATCAAGAACTTGGTATACTTTTAAAGATAAATAGTTATCAAACAATTGATAAAGTATATAATGATGTAGAAGAGGCTTTAAAATGGTCATAA", "aa": "MIEKSQEVYNEHAKKFPNIIMLGAPGAGKGSIATKMVQNNDYVQISTGDMFRQEIKNQTPLGLKIKSILDSGAYVDDCITNQLVEKKLTELVRENKHFILDGYPRTLAQAKFLLSLANVGIKIDKVILLEITDKQIIDRLSKRRICLNCKTIYHLQSFPPLPGDLCIKCHEKVTKRPDDEPEVIKKRLSIYNEQTKCLINFYQELGILLKINSYQTIDKVYNDVEEALKWS", "aa_hexdigest": "9e30a9b09bc52fda566890f295e3c6cf", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI000376F4E5", "ncbi_nrp_id": "WP_020002415.1", "uniref100_id": "UniRef100_UPI000376F4E5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000376F4E5", "RefSeq:WP_020002415.1", "UniRef:UniRef100_UPI000376F4E5" ] }, "ips": { "uniref100_id": "UniRef100_UPI000376F4E5", "uniref90_id": "UniRef90_D1J866" }, "psc": { "uniref90_id": "UniRef90_D1J866", "gene": "Adk", "product": "adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q6KI35", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005524", "GO:0006139", "GO:0019205" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KI35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KI35" ], "product": "Adenylate kinase" }, "genes": [ "adk" ], "id": "DFOKDJOJIG_676", "locus": "GCA013367855_00662" }, { "type": "cds", "sequence": "contig_1", "start": 379037, "stop": 379468, "strand": "-", "frame": 3, "gene": "secY", "product": "SecY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD3D78", "UniRef:UniRef90_UPI0021BD3D78" ], "nt": "TTGCAATTTACTCAACCACTTGGATTCTTTCTATTTATTTTAATTACATTTGGATTAACAATATTAATGGGAATCCAACAATCAAGAATTGATAAAATTAGTGAAGATTTTGCTAAAAACAGTACTTTTATTCCTGGCGTTAGACCCGGAGAACAAACAGAAGATTATTTATTGAATGTAATTTTACGTTTATCATTCTTTTCTTCAGGATATTTATTGCTGCTAGGCGCTTTACAATTTATTCAACAAATGCTAGGCATGCCAGCCTCAATAGCATTTGGTGGTACAAGTATAATGATACTAGTTTCAACGGCTTATGAAACTGTTCAACAAATTAAAGCCCGCTACAAGTCACAAGAATTATCAAGAAAGAGAAAAATGATAAGAGAATTAAAAGAAATGTATGGAGAGGAAGAAGAAGATTTAATATGA", "aa": "MQFTQPLGFFLFILITFGLTILMGIQQSRIDKISEDFAKNSTFIPGVRPGEQTEDYLLNVILRLSFFSSGYLLLLGALQFIQQMLGMPASIAFGGTSIMILVSTAYETVQQIKARYKSQELSRKRKMIRELKEMYGEEEEDLI", "aa_hexdigest": "85abb75e04e5391b92af8f76c1e9a417", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "secY" ], "truncated": "3-prime", "pseudogene": { "start": 379037, "stop": 379768, "observations": { "mutation": [ 379483 ], "stop": [ 379483 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021BD3D78", "query_cov": 1.0, "subject_cov": 0.5296296296296297, "identity": 0.867, "score": 243.0, "evalue": 5.13e-79, "gene_start": 1, "gene_end": 143, "reference_start": 127, "reference_end": 269, "reference_sequence": "MLWFFVGIASGLIPSFKSAFQYYIPSAKQTNLVLQEIINYAVYILVYLLTIFIVVIFTIAERRIPIQQVGAGLSKNAEELSYLPLKANPAGIMSVIFSMMILGVPTMIANMLNPNTSRYYQWVYANLQFTQPLGFFLFILITFGLTILMGIQQSKIDKISEDFAKSSTYIPGIRPGEQTETYLLNVVLRLSFFSSGFLIVLGSLQFVQQMIGMPASIAFGGTTVMILVSTAYETIQQIKARYKSQELARKRRTIRELKEMYGEEEEDLIW" }, "paralog": false, "identity": 0.8559670781893004, "score": 414.0, "evalue": 5.6e-149, "description": "Internal stop codon at 379483. Nonsense mutation around 379483.", "psc": { "uniref90_id": "UniRef90_UPI0021BD3D78", "product": "SecY", "uniref50_id": "UniRef50_UPI0021BD3D78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021BD3D78", "UniRef:UniRef50_UPI0021BD3D78" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021BD3D78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD3D78" ] } }, "id": "DFOKDJOJIG_677", "locus": "GCA013367855_00663" }, { "type": "cds", "sequence": "contig_1", "start": 379481, "stop": 380254, "strand": "-", "frame": 3, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952", "SO:0001217", "UniRef:UniRef50_A0A448ZXS5", "UniRef:UniRef90_D1J867" ], "nt": "TTGAATTTAGTAGGTGGCGGCGGATTAAGACAATTCTCTGTTGTCGCGCTAGGAATAGGGCCGTTTATATCATCTAGTTTAGTTATGATGATATTGCAAACTAAAGCATTCCCGGCCATTCACCGTTTAAGTCAATCTGGTCCTCAAGGCCGGATTAAAATAAATTTCATTACTTACGGAGTTACTTTTGTTTTTGCAATAGTTCAAGCATATTTGATAACAAAAGCATTAGTTAATCCTCGTCAAGGATTTGGAATTACTTTTTCACCAAAATTAGAAAAATTATTTGGACAAAGTGGAATGGTAGCATATCAATATTTCATTTTGCCTGCAATATTAGTAGCTGGCTCATTCTTTTCATTGTTCTTATCAGAACAAATAACTAATAGAGGCGTTGGCAATGGAACGAGTTTAATAATCTTTGTTGGTATAGCAAGTGCTTTAATTCCTTCATTCAAGAGTGCATTTAAATTTTATGTTCCATCAGCTCAACAAAATAATTTAGTTTTACAAGAAGCAATAAATTACGCAGTATATATCTTTTGCTATTTACTTGTAATATTTATTGTTGTAATATTTACAATTGCAGAAAGACATATTCCAATTCAACAAGTTGGTGCGGGTCTTTCTAAAAACGAAAAAGAATTGAGTTATTTACCTATTAAAGCAAATCCTGCTGGTATCATGTCAGTTATCTTCTCAATGATGATATTGAGTGTGCCAACAATGATTGCAAACTTATTTGATCCAAATACAAGCAAGTATTATCAATGA", "aa": "MNLVGGGGLRQFSVVALGIGPFISSSLVMMILQTKAFPAIHRLSQSGPQGRIKINFITYGVTFVFAIVQAYLITKALVNPRQGFGITFSPKLEKLFGQSGMVAYQYFILPAILVAGSFFSLFLSEQITNRGVGNGTSLIIFVGIASALIPSFKSAFKFYVPSAQQNNLVLQEAINYAVYIFCYLLVIFIVVIFTIAERHIPIQQVGAGLSKNEKELSYLPIKANPAGIMSVIFSMMILSVPTMIANLFDPNTSKYYQ", "aa_hexdigest": "36e165d8b8faf321a6d6ce6ece0a03ce", "start_type": "TTG", "rbs_motif": "AGGAG(G)/GGAGG", "genes": [ "secY" ], "truncated": "both", "pseudogene": { "start": 379178, "stop": 380503, "observations": { "mutation": [ 379483, 380395, 380398, 380407 ], "stop": [ 379483, 380395, 380398, 380407 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J867", "query_cov": 1.0, "subject_cov": 0.5255623721881391, "identity": 0.996, "score": 489.0, "evalue": 4.52e-171, "gene_start": 1, "gene_end": 257, "reference_start": 84, "reference_end": 340, "reference_sequence": "MARKTKLEKEISQEKIERKLALDKFLVDKKNTWNEWWKNHNLFKKILFTLFIITLFLATGTITIPGVNLANPDSLTQGDFFGILNLVGGGGLRQFSVVALGIGPFISSSLVMMILQTKAFPAIHRLSQSGPQGRIKINFITYGVTFVFAIVQAYLITKALVNPRQGFGITFSPKLEKLFGQSGMVAYQYFILPAILVAGSFFSLFLSEQITNRGVGNGTSLIIFVGIASALIPSFKSAFKFYVPSAQQNNLVLQEAINYAVYIFCYLLVIFIVVIFTIAERHIPIQQVGAGLSKNEKELSYLPIKANPAGIMSVIFSMMILSVPTMIANLFDPNTSKYYQWVYNNLQFTQPLGFFLFILITFGLTILMGIQQSRIDKISEDFAKNSTFIPGVRPGEQTEDYLLNVILRLSFFSSGYLLLLGALQFIQQMLGMPASIAFGGTSIMILVSTAYETVQQIKARYKSQELSRKRKMIRELKEMYGEEEEDLIW" }, "paralog": false, "identity": 0.9909297052154195, "score": 839.0, "evalue": 2.62e-311, "description": "Internal stop codon at 379483, 380395, 380398, 380407. Nonsense mutation around 379483, 380395, 380398, 380407.", "psc": { "uniref90_id": "UniRef90_D1J867", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_A0A448ZXS5", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0015031", "GO:0016020", "GO:0043952" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J867", "UniRef:UniRef50_A0A448ZXS5", "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXS5" ], "product": "Protein translocase subunit SecY" } }, "id": "DFOKDJOJIG_678", "locus": "GCA013367855_00664" }, { "type": "cds", "sequence": "contig_1", "start": 380405, "stop": 380503, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGAAAAACTAAGTTGGAAAAAGAGATTAGTCAAGAAAAGATCGAGCGCAAATTAGCACTAGATAAGTTTTTAGTTGATAAAAAAAACACTTGA", "aa": "MARKTKLEKEISQEKIERKLALDKFLVDKKNT", "aa_hexdigest": "52b053d98925f993c9317f2b09f4dc46", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3832.5150000000012, "isoelectric_point": 9.775808525085449 }, "id": "DFOKDJOJIG_679", "locus": "GCA013367855_00665" }, { "type": "cds", "sequence": "contig_1", "start": 380503, "stop": 380937, "strand": "-", "frame": 1, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_012855556.1", "SO:0001217", "UniParc:UPI0001BC2246", "UniRef:UniRef100_D1J868", "UniRef:UniRef50_Q601J6", "UniRef:UniRef90_D1J868" ], "nt": "ATGCAATTACATAATTTAAAACCAACTCCAGGGGCAAGAAAAGACAAACACCGTGTTGGTCGTGGTCATGCTGCTGGAAAAGGAAAACAAGCCGGTAGAGGACAAAGTGGTCAAACAAAAAGAAGCAAAGTAAGACTAGGATTTGAAGGTGGCCAATTGCCACTATTTAGAAGAATTCCAAAACGTGGATTTAGCAATGTAAATCACGTTGAATTCCAAGTAGTAAATCTAGCAGATTTACAATCAAAATATCAAGATGGTGAAGTAGTTTCTTACGAAACACTTGCTGCAAGAAACTTGATTAAAGGAACATTACCAGTTAAAATTTTAGCTAAGGGTTCTTTAACTAAAAAACTTAATGTACAAATTCCAACACTTTCACAATCCGCTATTGAAGCTATTGAAAAAGTCGGAGGAAAAATAGAGGTTAAATAA", "aa": "MQLHNLKPTPGARKDKHRVGRGHAAGKGKQAGRGQSGQTKRSKVRLGFEGGQLPLFRRIPKRGFSNVNHVEFQVVNLADLQSKYQDGEVVSYETLAARNLIKGTLPVKILAKGSLTKKLNVQIPTLSQSAIEAIEKVGGKIEVK", "aa_hexdigest": "e48e82061d297ab366d048e8a4e1e848", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2246", "ncbi_nrp_id": "WP_012855556.1", "uniref100_id": "UniRef100_D1J868", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2246", "RefSeq:WP_012855556.1", "UniRef:UniRef100_D1J868" ] }, "ips": { "uniref100_id": "UniRef100_D1J868", "uniref90_id": "UniRef90_D1J868" }, "psc": { "uniref90_id": "UniRef90_D1J868", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_Q601J6", "cog_id": "COG0200", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q601J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q601J6" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "DFOKDJOJIG_680", "locus": "GCA013367855_00666" }, { "type": "cds", "sequence": "contig_1", "start": 380947, "stop": 381615, "strand": "-", "frame": 1, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02988", "RefSeq:WP_020002417.1", "SO:0001217", "UniParc:UPI000367CA6D", "UniRef:UniRef100_A0A454CA16", "UniRef:UniRef50_L0RWI9", "UniRef:UniRef90_D1J869" ], "nt": "ATGGCTGAAGTAGAAAAAAATATTAAAAATTCAGAAAAACCTGAATTAAAAGAAAATAAAACAGAAGGCACAAAGGTTGTTTCTTTTAAAAGAGAAAGATCAAATTCAAAAGATCTAAAATCAAGCGAAAATAAACCAAAACAATTCCAAAATCGTAATCAAGATCATTCAGCAAATCAAGCTCAAGCAAAACAATTCGAAGAAAAAGTTATTGACATTGCAAGAGTTACAACTGTTGTTAAGGGTGGACGTAGATTTTCATTCTCAGCTTATGTTGTTGTTGGTGACAAAAAAGGAAAAGTTGGATTTGGACACGGAAAAGCTAATGAAGTCCAAGATGCAATTAAAAAAGCAGTTAAAGATGCACAAAAAAATGTTTTCTCAGTTCCAATTTCAAATGGAACAATCCCTCATGAAATTCAAGATAAATTCTTAGCATCAAGAATCCAATTACGTCCAGCTCCAAAAGGTGCCGGAATCATTGCTTCAAACACCGTTCGTGCTGTTGTTGAATTAGCAGGTTACACCGACATTTCAACAAAAACTTATGGTTCAAGAACAAAACAAAACATTGTCCAAGCTACTATTAAGGCACTTAAAAAAGTTAGAACTGTTGAAGAAATCGCAAAATTACGCGATATTGATGTAAATCATCTATTATCAAAATAA", "aa": "MAEVEKNIKNSEKPELKENKTEGTKVVSFKRERSNSKDLKSSENKPKQFQNRNQDHSANQAQAKQFEEKVIDIARVTTVVKGGRRFSFSAYVVVGDKKGKVGFGHGKANEVQDAIKKAVKDAQKNVFSVPISNGTIPHEIQDKFLASRIQLRPAPKGAGIIASNTVRAVVELAGYTDISTKTYGSRTKQNIVQATIKALKKVRTVEEIAKLRDIDVNHLLSK", "aa_hexdigest": "bcbc4611471dda464e1e9604f871d769", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI000367CA6D", "ncbi_nrp_id": "WP_020002417.1", "uniref100_id": "UniRef100_A0A454CA16", "db_xrefs": [ "SO:0001217", "UniParc:UPI000367CA6D", "RefSeq:WP_020002417.1", "UniRef:UniRef100_A0A454CA16" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CA16", "uniref90_id": "UniRef90_D1J869" }, "psc": { "uniref90_id": "UniRef90_D1J869", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_L0RWI9", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_L0RWI9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RWI9" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "DFOKDJOJIG_681", "locus": "GCA013367855_00667" }, { "type": "cds", "sequence": "contig_1", "start": 381615, "stop": 381962, "strand": "-", "frame": 2, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "COG:COG0256", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_175972278.1", "SO:0001217", "UniParc:UPI00158C566E", "UniRef:UniRef100_UPI00158C566E", "UniRef:UniRef50_A0A6M4JHS6", "UniRef:UniRef90_D1J870" ], "nt": "ATGTTATCAAGAAATTTAAAACGTAAAGTAAAACACTTAAAGATTACTAACAAATTGTCAAAAGGAACTGCATTAGTTCCTAGAGTTGTTGTTTTTAAATCTTTACATGCATTTTATGCACAAGCAGTAAATGATGAATTGCACACCACAATCGCTTCATCATCAACACAAATACTAGAAAATAAAGCAAACAATATTGATGCAGTTAAATTAGTTGCAAAAGATTTTGCTAAAAAATTATTGGCAATCAATGTAAAATCAATAGTGTTTGATCGTTCAGGATACATATACCACGGAAAATTAGCAGCTTTTGCTGATACTCTAAGAGAAGAAGGGATTAAATTCTAA", "aa": "MLSRNLKRKVKHLKITNKLSKGTALVPRVVVFKSLHAFYAQAVNDELHTTIASSSTQILENKANNIDAVKLVAKDFAKKLLAINVKSIVFDRSGYIYHGKLAAFADTLREEGIKF", "aa_hexdigest": "a5f1ce7924b0bbaab3c1b18b8d5beba0", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158C566E", "ncbi_nrp_id": "WP_175972278.1", "uniref100_id": "UniRef100_UPI00158C566E", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C566E", "RefSeq:WP_175972278.1", "UniRef:UniRef100_UPI00158C566E" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C566E", "uniref90_id": "UniRef90_D1J870" }, "psc": { "uniref90_id": "UniRef90_D1J870", "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_A0A6M4JHS6", "cog_id": "COG0256", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6M4JHS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4JHS6" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "DFOKDJOJIG_682", "locus": "GCA013367855_00668" }, { "type": "cds", "sequence": "contig_1", "start": 381984, "stop": 382523, "strand": "-", "frame": 2, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "RefSeq:WP_012855559.1", "SO:0001217", "UniParc:UPI0001BC2249", "UniRef:UniRef100_D1J871", "UniRef:UniRef50_Q4A5D6", "UniRef:UniRef90_D1J871" ], "nt": "ATGTCTAGAATCGGAAAAAGAGTTTTAGAAATACCAAAAGATGTTGAAGTTAAATTAGATAAAAACCATATCGAAGTAAAAGGCAAATTAGGAGAATTGAGTTATACATTCTCACCATTAATTTCAGTAGTTGTTGAAAACAACGAAATTAAGACATTGAGATCTAACGAAGAAAAATCTACAAAACAATTACACGGAACAACAAATGCAAACATTAAAAATATGTTAATAGGTGTTTCAAGTGGTTACAAAAAAGAAATTGAAATTAAAGGTGTAGGTTACAAAGCCACCTTAAAGGGAAATACAATTGAAATTATTGCTGGTTATTCACATGCTGTAAATTTAGAAGTTCCATCAAATCTAAAAATTGAATTACCAAAACCAACCAATATTATTATTTCAGGAATTGACAAACAAGCTGTTGGTGAATTTGCTGCAAATATTCGTGATGTAAGACGTCCAAGTCCATATTCTGGAAAAGGTATTATGTATAAAGATGAAGTTATTAGACGTAAGGAAGGAAAGACAGCTGCTAAATAG", "aa": "MSRIGKRVLEIPKDVEVKLDKNHIEVKGKLGELSYTFSPLISVVVENNEIKTLRSNEEKSTKQLHGTTNANIKNMLIGVSSGYKKEIEIKGVGYKATLKGNTIEIIAGYSHAVNLEVPSNLKIELPKPTNIIISGIDKQAVGEFAANIRDVRRPSPYSGKGIMYKDEVIRRKEGKTAAK", "aa_hexdigest": "e98ddf5827d0489bd840bf7f811c766a", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001BC2249", "ncbi_nrp_id": "WP_012855559.1", "uniref100_id": "UniRef100_D1J871", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2249", "RefSeq:WP_012855559.1", "UniRef:UniRef100_D1J871" ] }, "ips": { "uniref100_id": "UniRef100_D1J871", "uniref90_id": "UniRef90_D1J871" }, "psc": { "uniref90_id": "UniRef90_D1J871", "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_Q4A5D6", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5D6" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "DFOKDJOJIG_683", "locus": "GCA013367855_00669" }, { "type": "cds", "sequence": "contig_1", "start": 382548, "stop": 382958, "strand": "-", "frame": 2, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_Q6MSN9", "UniRef:UniRef90_D1J872" ], "nt": "ATGGCTATTATTATAGATCCAGTTGCAGATATGTTTGTACGTATTAAAAATGCAATTTCTCGTAAATATCATGAAGTTGTATTGCCACATTCAAACAAGAAAGCAAAAATTTTAGAAATTATTAAAAGAGAAGGATATATTGTTGATTTTGAAGTTGTAAAAAACGAAAAAACCAATTTTAAAGAAATAAAAGTTACTTTAAAATACAAAGGTATGAATCAAAATCAATCAGCAATTTCTGGTCTTAAAAAAGTTTCAAAACCTGGCTTAAAAGTATATTCATCTGCTGAAAAATTGCCTAGAGTTTTAAGTGGATATGGAACAGCTATTATTTCAACATCAAAAGGTTTATTAACAGATAAAGAAGCTCGTAAGGCTAATGTTGGTGGCGAAGTTATCGCCTTCATTTGA", "aa": "MAIIIDPVADMFVRIKNAISRKYHEVVLPHSNKKAKILEIIKREGYIVDFEVVKNEKTNFKEIKVTLKYKGMNQNQSAISGLKKVSKPGLKVYSSAEKLPRVLSGYGTAIISTSKGLLTDKEARKANVGGEVIAFI", "aa_hexdigest": "44621eacfa9ce74a148bdad8879e46d6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J872", "query_cov": 1.0, "subject_cov": 0.9927007299270073, "identity": 1.0, "score": 256.0, "evalue": 4.4e-86, "valid": true, "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q6MSN9", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MSN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MSN9" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "DFOKDJOJIG_684", "locus": "GCA013367855_00670" }, { "type": "cds", "sequence": "contig_1", "start": 382964, "stop": 383146, "strand": "-", "frame": 3, "gene": null, "product": "Small ribosomal subunit protein uS14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP7" ], "nt": "ATGGCAAGAACATCATTAAAGGTTAAAGCCGAACGCCAACCTAAATTTAAGGTTAGAAAATATACTCGTTGCCAACTTTGTGGCCGTGTTCACGCTGTATTGCGTAAATATAAAATTTGTAGAATTTGTTTTAGAAAATTAGCACATGAAGGAAAACTTCCTGGTGTTAAGAAAGCGAGCTGA", "aa": "MARTSLKVKAERQPKFKVRKYTRCQLCGRVHAVLRKYKICRICFRKLAHEGKLPGVKKAS", "aa_hexdigest": "4b14d9dfdd9d224ff7a908e546cd8f55", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A448ZVU2", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 0.85, "score": 106.0, "evalue": 2.85e-29, "valid": false, "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_Q9PQP7", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP7" ], "product": "Small ribosomal subunit protein uS14" }, "genes": [], "id": "DFOKDJOJIG_685", "locus": "GCA013367855_00671" }, { "type": "cds", "sequence": "contig_1", "start": 383473, "stop": 383718, "strand": "-", "frame": 1, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "nt": "ATGGCAAAACAAATTAAATTAGCATTAGAACAAAAATATTTAGACGAAATTCGTTTCGCGCTAGTTAAAGAATTTGGTTACACCAGTGTAATGCAAGCTCCTAAACTAGAAAAAATCGTTATTAACATGACTGCCGGTAACGAAGTTTCAAACTCAAAGGCAATCGAAGAAGTTATGGTTGAACTTGCTCAAATTACAGGTCAAAAACCATATCAAACAACTGCTAAGAAATCATTGGCTTCATGA", "aa": "MAKQIKLALEQKYLDEIRFALVKEFGYTSVMQAPKLEKIVINMTAGNEVSNSKAIEEVMVELAQITGQKPYQTTAKKSLAS", "aa_hexdigest": "7a5beec12a4a6cd8a099cdf0e8effdc6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002E1CF638", "query_cov": 0.8888888888888888, "subject_cov": 0.8571428571428571, "identity": 0.569, "score": 79.7, "evalue": 5.85e-18, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A2X3HCF5", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A2X3HCF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "DFOKDJOJIG_686", "locus": "GCA013367855_00672" }, { "type": "cds", "sequence": "contig_1", "start": 383796, "stop": 384068, "strand": "-", "frame": 2, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_A0A448ZW26", "UniRef:UniRef90_A0A454CAH0" ], "nt": "ATGGCTCAAGCTAAAATTAGAAAAAATGACATGGTATTAATTATTGCTGGTAAAGACAAAGGAAAATTTGCTGCTGTTTTATCAACAAATCAAAAAGAACAAACTGTTGTTTTAAAAGGCGTAAACATTAAAACTAAACACCATAAACCATCACAAGAAAATAATGATGGAAAAATTGAAAAGAAAGAATACCCAATTCATATTTCAAACGTTGCTTATTTGGTTAAAAAAGGTGCTCAAGGTCAAAAATCAATCGGTTCAAAAATTGGTTGA", "aa": "MAQAKIRKNDMVLIIAGKDKGKFAAVLSTNQKEQTVVLKGVNIKTKHHKPSQENNDGKIEKKEYPIHISNVAYLVKKGAQGQKSIGSKIG", "aa_hexdigest": "751bca10ea367ed894847567bc73e234", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_A0A454CAH0", "query_cov": 1.0, "subject_cov": 0.8108108108108109, "identity": 1.0, "score": 173.0, "evalue": 1.23e-54, "valid": true, "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_A0A448ZW26", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZW26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZW26" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "DFOKDJOJIG_687", "locus": "GCA013367855_00673" }, { "type": "cds", "sequence": "contig_1", "start": 384070, "stop": 384438, "strand": "-", "frame": 1, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02874", "RefSeq:WP_012855564.1", "SO:0001217", "UniParc:UPI0001BC229F", "UniRef:UniRef100_D1J876", "UniRef:UniRef50_A5FMZ0", "UniRef:UniRef90_D1J876" ], "nt": "ATGCTTAGTGAATTTTCAAGATGTAACGTTGCTGACAATTCAGGCGCAAAAGAAGTTATGATTATTAAAAACCTAGGTGGATCAATTGTTAGATCAACCAACATTGGTGATATTGTTGTTGTAACTATTAAAAAAGCTATTCCAAACGGAATTGTAAAAGAAGGCCAAGTTGTTAAAGCAGTTATAGTTAGAACAAAACGTGGTTTAATGCGTGAAAATGGTTCTCATATAAGATTTGATGACAATGCTGTTGTATTAATTAAAGAAGATAACTCATTAAGAGGAACTCGGGTATTTGGTCCAGTAGCAAGAGAATTACGTGAAAAAGGTTTTTTAAAGATTGTTTCATTAGCTCCGGAGGTTTTATAA", "aa": "MLSEFSRCNVADNSGAKEVMIIKNLGGSIVRSTNIGDIVVVTIKKAIPNGIVKEGQVVKAVIVRTKRGLMRENGSHIRFDDNAVVLIKEDNSLRGTRVFGPVARELREKGFLKIVSLAPEVL", "aa_hexdigest": "c87871ee0223ca5673069e09b0bae576", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC229F", "ncbi_nrp_id": "WP_012855564.1", "uniref100_id": "UniRef100_D1J876", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC229F", "RefSeq:WP_012855564.1", "UniRef:UniRef100_D1J876" ] }, "ips": { "uniref100_id": "UniRef100_D1J876", "uniref90_id": "UniRef90_D1J876" }, "psc": { "uniref90_id": "UniRef90_D1J876", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_A5FMZ0", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A5FMZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5FMZ0" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "DFOKDJOJIG_688", "locus": "GCA013367855_00674" }, { "type": "cds", "sequence": "contig_1", "start": 384431, "stop": 384715, "strand": "-", "frame": 3, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "COG:COG0186", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855565.1", "SO:0001217", "UniParc:UPI0001BC22A0", "UniRef:UniRef100_D1J877", "UniRef:UniRef50_Q98PZ1", "UniRef:UniRef90_D1J877" ], "nt": "ATGGAAACTACAGTTAGAGAAAACCGTCGTAAAACTTTAGTTGGAACAGTTGTATCAACTAAAAACCAAAAAACAATTACTGTTGTTGTAGAAATTTACGAAAAACACCCTCTTTATTCAAAACGTTTTAAAAAATCAAAGAAATTCGCAGTTCATGATGAACAAAATTTAGCTAAAGTTGGTGACATTGTTAAAATTCAAGAAACAAGACCTTTATCAAAAACCAAACACTTTAGACTAGTAGAAATCAAATCACACGCAATTGAAGGAGCAGAAAATGCTTAG", "aa": "METTVRENRRKTLVGTVVSTKNQKTITVVVEIYEKHPLYSKRFKKSKKFAVHDEQNLAKVGDIVKIQETRPLSKTKHFRLVEIKSHAIEGAENA", "aa_hexdigest": "ea20c26ef5b5db251866ea7f325bed04", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001BC22A0", "ncbi_nrp_id": "WP_012855565.1", "uniref100_id": "UniRef100_D1J877", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A0", "RefSeq:WP_012855565.1", "UniRef:UniRef100_D1J877" ] }, "ips": { "uniref100_id": "UniRef100_D1J877", "uniref90_id": "UniRef90_D1J877" }, "psc": { "uniref90_id": "UniRef90_D1J877", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_Q98PZ1", "cog_id": "COG0186", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PZ1" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "DFOKDJOJIG_689", "locus": "GCA013367855_00675" }, { "type": "cds", "sequence": "contig_1", "start": 384715, "stop": 384915, "strand": "-", "frame": 1, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855566.1", "SO:0001217", "UniParc:UPI0001BC22A1", "UniRef:UniRef100_D1J878", "UniRef:UniRef50_A0A3B0PQ96", "UniRef:UniRef90_D1J878" ], "nt": "ATGACATTTGCAGAATTAAAAGAAAAATCACTTGACGAACTAAATAAGTTGGTTGCTGATTTAAGAGCAGAACTATTCTCATTACGTTTCAAAAATGCTACAAGACAATTAGACGAAACCCACAAAATCCAATTAGTTAAAAAAGATATCGCAAGAACTTTAACAGCAATTAAAGCAAAAACTTTAGAAGGGAATAAATAA", "aa": "MTFAELKEKSLDELNKLVADLRAELFSLRFKNATRQLDETHKIQLVKKDIARTLTAIKAKTLEGNK", "aa_hexdigest": "cb17ed1367064946a7cec20043621673", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A1", "ncbi_nrp_id": "WP_012855566.1", "uniref100_id": "UniRef100_D1J878", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A1", "RefSeq:WP_012855566.1", "UniRef:UniRef100_D1J878" ] }, "ips": { "uniref100_id": "UniRef100_D1J878", "uniref90_id": "UniRef90_D1J878" }, "psc": { "uniref90_id": "UniRef90_D1J878", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_A0A3B0PQ96", "cog_id": "COG0255", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PQ96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PQ96" ], "product": "Large ribosomal subunit protein uL29" }, "genes": [ "rpmC" ], "id": "DFOKDJOJIG_690", "locus": "GCA013367855_00676" }, { "type": "cds", "sequence": "contig_1", "start": 385089, "stop": 385196, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00252.24" ], "nt": "ATGGGACGTGAAGGAAAGGTTATTATCAAAATATTCCCTCACATGTCAAAAACTAGTAAACCTATTGGTGTTCGTATGGGATCTGGTAAGGGTAGCCCAGATCAATGA", "aa": "MGREGKVIIKIFPHMSKTSKPIGVRMGSGKGSPDQ", "aa_hexdigest": "06b42c6d46d5e2a7d1d7dd09ca724493", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00252.24", "name": "Ribosomal_L16", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 4.2450231512247687e-10, "score": 35.33918762207031, "start": 3, "stop": 34 } ], "seq_stats": { "molecular_weight": 3755.4388000000017, "isoelectric_point": 10.564772605895993 }, "id": "DFOKDJOJIG_691", "locus": "GCA013367855_00677" }, { "type": "cds", "sequence": "contig_1", "start": 385358, "stop": 385519, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGAAGCATGTCTTTCACCGCCTTTTTTAAATTCTCTTTTACGTGGAGCTTCTTTTACTTCTGCTTCACTTGTTTTAGGTGATAAAATTTCGCCTAATGAAACTCATACTTTAACTCCTAAGATACCATATGTTGTTAAGGCTGTTGTTACAGCATAA", "aa": "MVEACLSPPFLNSLLRGASFTSASLVLGDKISPNETHTLTPKIPYVVKAVVTA", "aa_hexdigest": "543cba1b7128546045346a0888109506", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5585.493300000001, "isoelectric_point": 7.939813423156738 }, "id": "DFOKDJOJIG_692", "locus": "GCA013367855_00678" }, { "type": "cds", "sequence": "contig_1", "start": 385465, "stop": 385725, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00189.25" ], "nt": "TTGCTAGCAGAAGGAATTGCAATTAAATTAGAAAATCGTGGTAGTTTTAGACTAGCACAAAAATTTGCAATTAAAGCAGCATTAAAAGCTGGCGCAAAAGGTATTAAAACTTCAGTAGCTGGACGTCTAAATGGCGTTGATATGGCTAGAACAGAAGGATATACCGAAGGTGAAATGAAATTACATACTTTAAGACAAGATGTTGATTATGCTGTAACAACAGCCTTAACAACATATGGTATCTTAGGAGTTAAAGTATGA", "aa": "MLAEGIAIKLENRGSFRLAQKFAIKAALKAGAKGIKTSVAGRLNGVDMARTEGYTEGEMKLHTLRQDVDYAVTTALTTYGILGVKV", "aa_hexdigest": "12fab75bd1d3b9296d32e0538593a4dd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00189.25", "name": "Ribosomal_S3_C", "length": 81, "aa_cov": 0.9418604651162791, "hmm_cov": 1.0, "evalue": 2.6110966358950456e-32, "score": 106.8982925415039, "start": 6, "stop": 86 } ], "seq_stats": { "molecular_weight": 9199.638499999995, "isoelectric_point": 9.752470970153805 }, "id": "DFOKDJOJIG_693", "locus": "GCA013367855_00679" }, { "type": "cds", "sequence": "contig_1", "start": 386079, "stop": 386468, "strand": "-", "frame": 2, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "COG:COG0091", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02890", "RefSeq:WP_012855569.1", "SO:0001217", "UniParc:UPI0001BC22A4", "UniRef:UniRef100_Q8GM55", "UniRef:UniRef50_Q98PY6", "UniRef:UniRef90_Q8GM55" ], "nt": "ATGGCTAAAGAAATTTTACAAAATTCAGCACATGCATCAGTTCGCATGCAAAGAATTTCACCACGTAAAGCAAGATTAGTAGCAGATTTAATTAGATATAAATCAGCAACTCAAGCAATAGTTATTCTAAAACATACACACAAAAAAGCGTCAGAAATTATTCTAAAACTTCTAAATTCAGCAATTGCTAATGCAACAAATAATGCTGGACTTGATGCAACTAAACTTTATGTAACTACCATTTTAGTAAATGATGGACCTACACTAAAAAGATTCCAACCTCACTCACGTGGACGTGCTTATGCAATTTTAAAAAGAACAAGTCATTTCTTTATTGAATTAACAGAAATCAATAATGTTGAAGAAATCAACAAAAAAGGAGATAAATAA", "aa": "MAKEILQNSAHASVRMQRISPRKARLVADLIRYKSATQAIVILKHTHKKASEIILKLLNSAIANATNNAGLDATKLYVTTILVNDGPTLKRFQPHSRGRAYAILKRTSHFFIELTEINNVEEINKKGDK", "aa_hexdigest": "7a8b68311e501c927669bb50a65848bd", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC22A4", "ncbi_nrp_id": "WP_012855569.1", "uniref100_id": "UniRef100_Q8GM55", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A4", "RefSeq:WP_012855569.1", "UniRef:UniRef100_Q8GM55" ] }, "ips": { "uniref100_id": "UniRef100_Q8GM55", "uniref90_id": "UniRef90_Q8GM55" }, "psc": { "uniref90_id": "UniRef90_Q8GM55", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_Q98PY6", "cog_id": "COG0091", "cog_category": "J", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY6" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "DFOKDJOJIG_694", "locus": "GCA013367855_00680" }, { "type": "cds", "sequence": "contig_1", "start": 386643, "stop": 386747, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTTCATTAAAGAAAGCTCCCTTTGTTGATGATCATTTAATGAAGAAAGTTTTGGCTATTATTGAAAATAAATCACCAAAACGTCCAATTAAAACATGA", "aa": "MARSLKKAPFVDDHLMKKVLAIIENKSPKRPIKT", "aa_hexdigest": "5da938756da5c11a053df77e46912a7d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3904.734700000001, "isoelectric_point": 10.383809852600098 }, "id": "DFOKDJOJIG_695", "locus": "GCA013367855_00681" }, { "type": "cds", "sequence": "contig_1", "start": 386834, "stop": 387592, "strand": "-", "frame": 3, "gene": null, "product": "Large ribosomal subunit protein uL2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "nt": "ATGGCTATTAAAAAATTTAAAGCATATACCAATGGCCGTCGTAATATGTCTTCTCTTGATTATCAAGCAAATTTAACAGGACATGCTCCTGAAAAATCATTGCTTGTTGCTCTTCCAACAAAAGCAGGAAGAAACAATCAAGGTAAAATCACTACCCGTCATCAAGGTGGTAGATTGAAAAGATTCTATCGTATCATTGATTTTAAACGTCAAAAAGATGGAATCCCAGCTACTGTAAAAACTATTGAATATGACCCTAATCGTTCAGCTAATATTTCATTAGTTGTTTATAGAGATGGTGAAAAAAGATATATTTTAAGCCCAAAAGGTATCAAAATTGGACAAGTTATTTTGTCAGGTGATAATGTTGACATTCAAGTTGGTAACCATTTACCATTAGCCAACATTCCTGAAGGTACATTTGTTCACAATATTGAATTACAACCAAGACAAGGTGGAATCATTGCTAGATCAGCTGGATCATCAGCACAAATCTTAGGTAAAGATGAATCAGGTCGTTATGTAATTCTAAGATTAAAATCAGGCGAAGTTAGAAAAGTTTTAGCAATTAATCGTGCAACAGTTGGTGAAGTAGGAAACGAAGAACACTCACTTGTTAATATTGGTAAGGCAGGACGTAACCGTTTAAGAGGAATTAGACCAACAGTTCGTGGTTCAGCTATGAACCCTAATGATCACCCACATGGTGGTGGTGAAGGTAGACAACCTATTGGTAGAAAGAGTCCTATGACTCCATGA", "aa": "MAIKKFKAYTNGRRNMSSLDYQANLTGHAPEKSLLVALPTKAGRNNQGKITTRHQGGRLKRFYRIIDFKRQKDGIPATVKTIEYDPNRSANISLVVYRDGEKRYILSPKGIKIGQVILSGDNVDIQVGNHLPLANIPEGTFVHNIELQPRQGGIIARSAGSSAQILGKDESGRYVILRLKSGEVRKVLAINRATVGEVGNEEHSLVNIGKAGRNRLRGIRPTVRGSAMNPNDHPHGGGEGRQPIGRKSPMTP", "aa_hexdigest": "2d1053e9b4b069d6e507646decf318a5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3M0A1P8", "query_cov": 1.0, "subject_cov": 0.896797153024911, "identity": 0.897, "score": 457.0, "evalue": 1.04e-161, "valid": false, "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [], "id": "DFOKDJOJIG_696", "locus": "GCA013367855_00682" }, { "type": "cds", "sequence": "contig_1", "start": 387687, "stop": 388118, "strand": "-", "frame": 2, "gene": "rplW", "product": "50S ribosomal protein L23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855572.1", "SO:0001217", "UniParc:UPI00000BF6FA", "UniRef:UniRef100_D1J884", "UniRef:UniRef50_Q98PY3", "UniRef:UniRef90_D1J884" ], "nt": "ATGAATATAAATGAAGTTATTAAATACCCACTTTTAACAGAAAAAAGTGAATTATCACGTGAAAATAAAAACGTTTATACATTTGTAGTTGATAAAAGAACAAACAAAATCGAAGTTAAAAAAGCAGTTGAATTTATTTTTGATGTAAAAGTATTAAAAGTTAACATTACTAATTACGATAAAAAACCTGCTAAATTAGGACGTTTCTCAGGTTTTAAAAATTCAGTTAAAAAAGCAACTGTATATTTAGCAAAAGATTCAAAAATTATTCTATTTGCTGAAGAATTAAAGAAAGCTAACAAAGAAGCTGAAAAAGCAACAGCAGAAAAAGAAGTTAAAGAAGAAAAACCTGCTGAACCTACCGCTGCAGAATTAAAAGCAGCAGAAAAAATTAAAGCAGCTGAAGCTAAAAAAGCTGCTGAAAACAAATAG", "aa": "MNINEVIKYPLLTEKSELSRENKNVYTFVVDKRTNKIEVKKAVEFIFDVKVLKVNITNYDKKPAKLGRFSGFKNSVKKATVYLAKDSKIILFAEELKKANKEAEKATAEKEVKEEKPAEPTAAELKAAEKIKAAEAKKAAENK", "aa_hexdigest": "e9516e60a03d444412cf50f35f7ebe06", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00000BF6FA", "ncbi_nrp_id": "WP_012855572.1", "uniref100_id": "UniRef100_D1J884", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BF6FA", "RefSeq:WP_012855572.1", "UniRef:UniRef100_D1J884" ] }, "ips": { "uniref100_id": "UniRef100_D1J884", "uniref90_id": "UniRef90_D1J884" }, "psc": { "uniref90_id": "UniRef90_D1J884", "gene": "rplW", "product": "50S ribosomal protein L23", "uniref50_id": "UniRef50_Q98PY3", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY3" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "DFOKDJOJIG_697", "locus": "GCA013367855_00683" }, { "type": "cds", "sequence": "contig_1", "start": 388118, "stop": 388255, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCTTATCAGCAAGAAACTTACCAAATGTAACTGTAACAAAAGTAACTAGTTTATCAGTTGAATCTTTATTGGCGGCAGATGTTTTAGTTATTAGTTCAGATGATGTTAAATACTTAGAAGGATTGGTTAAATAA", "aa": "MFLSARNLPNVTVTKVTSLSVESLLAADVLVISSDDVKYLEGLVK", "aa_hexdigest": "8430c512fd0c8253469c444377393a46", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4836.5984, "isoelectric_point": 4.8610639572143555 }, "id": "DFOKDJOJIG_698", "locus": "GCA013367855_00684" }, { "type": "cds", "sequence": "contig_1", "start": 388901, "stop": 389086, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314D514", "UniRef:UniRef90_UPI001314D514" ], "nt": "ATGGCAACAAAAAATGTAACATTAGAAAAATTCTACATTTCTGAAAAATTTGATGAAAATCGTAATATTTCATTTAATTTAAAAAAGGCTAACCAAAAATCATTCAAGAATTTTGAAAATTTTTCAGAAGCATTAACAGAATTTATTGAACTTTCTGAAAAATCAAAAAACAATACTAGAGTATGA", "aa": "MATKNVTLEKFYISEKFDENRNISFNLKKANQKSFKNFENFSEALTEFIELSEKSKNNTRV", "aa_hexdigest": "65cd46d9cd54cbf3847c176a252340c8", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 388789, "stop": 389086, "observations": { "insertion": [ 388822 ], "mutation": [ 388903 ], "stop": [ 388903 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314D514", "query_cov": 1.0, "subject_cov": 0.6224489795918368, "identity": 1.0, "score": 115.0, "evalue": 3.69e-32, "gene_start": 1, "gene_end": 61, "reference_start": 1, "reference_end": 61, "reference_sequence": "MATKNVTLEKFYISEKFDENRNISFNLKKANQKSFKNFENFSEALTEFIELSEKSKNNTRVWFHRDGAYRGSVGLEKAKLILEKVTGEKLKMKKQSTT" }, "paralog": false, "identity": 0.9897959183673469, "score": 184.0, "evalue": 5.37e-62, "description": "Internal stop codon at 388903. Frameshift due to insertion around 388822. Nonsense mutation around 388903.", "psc": { "uniref90_id": "UniRef90_UPI001314D514", "uniref50_id": "UniRef50_UPI001314D514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314D514", "UniRef:UniRef50_UPI001314D514" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314D514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314D514" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7242.053499999998, "isoelectric_point": 9.04905643463135 }, "id": "DFOKDJOJIG_699", "locus": "GCA013367855_00685" }, { "type": "cds", "sequence": "contig_1", "start": 389086, "stop": 389412, "strand": "-", "frame": 1, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_D1J886" ], "nt": "ATGCCAGGACATTTAGGAGCTGAACAAGTAACTGTTCAAAACCTAGAAGTTGTTAAAGAAGATGCAGAAAACAATATTTTATTGATCAAGGGATCAGTTCCTGGTGCTAAAGGAGCATTCTTAATCATTAAAACTGCTAAGAAATCAATTAAGAATAAACCAGTTATTAAATTATTGAATCTAAAAGAAGCTTTGGCTAAAAATGAAATCTTTACTCAAGCTAAAAAATACAATCTTGAATTAAACATGGATATGTCATTAAAAGAAATGAAAGCTGCTTTGGATGAAGCAATTGCTAAGGCCGGAGAAGAAGGAGAAAATAAATAA", "aa": "MPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK", "aa_hexdigest": "8579bc1a82bf19d1b9aee04b89886a80", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rplC" ], "truncated": "3-prime", "pseudogene": { "start": 389086, "stop": 389712, "observations": { "mutation": [ 389427, 389526 ], "stop": [ 389427, 389526 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J886", "query_cov": 1.0, "subject_cov": 0.40298507462686567, "identity": 1.0, "score": 201.0, "evalue": 3.99e-63, "gene_start": 1, "gene_end": 108, "reference_start": 161, "reference_end": 268, "reference_sequence": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVWKGMTMPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK" }, "paralog": false, "identity": 0.9903846153846154, "score": 400.0, "evalue": 8.29e-144, "description": "Internal stop codon at 389427, 389526. Nonsense mutation around 389427, 389526.", "psc": { "uniref90_id": "UniRef90_D1J886", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J886", "UniRef:UniRef50_Q98PY0", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "DFOKDJOJIG_700", "locus": "GCA013367855_00686" }, { "type": "cds", "sequence": "contig_1", "start": 389524, "stop": 389892, "strand": "-", "frame": 1, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_D1J886" ], "nt": "ATGAAAGGAATCTTAGGAAGAAAAGTTGGGATGACTCAACTATTTACTTCAGAAGGTAAATTAATACCTGTGACAGTAGTAGAAGTTAAACCAAATGTTGTTACTAAGGTTTTAACATCAGAAAAAAATGGTTATGTTGCTCTTCAATTAGCAACAGAAGATAAAAAATCTTCAAGAATCATTAAACCTGAAGTTAATTACTTTAAACAAGCAAATACAACACCTAAGCGCTTCGTAAAAGAAATCCGTGATATGTCAGGATTTTCATTAGGTGATACTGTAGATGCTTCATTATTTACCGCTGGAGAAGTTGTTGACGTAACAGCTATTTCTAAAGGTAAAGGATTTGCGGGTACCATCAAGAGATGA", "aa": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKR", "aa_hexdigest": "565ff623fc584ade78acfc33a3752bd4", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "rplC" ], "truncated": "5-prime", "pseudogene": { "start": 389221, "stop": 389892, "observations": { "mutation": [ 389427, 389526 ], "stop": [ 389427, 389526 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J886", "query_cov": 1.0, "subject_cov": 0.4552238805970149, "identity": 1.0, "score": 233.0, "evalue": 1.81e-75, "gene_start": 1, "gene_end": 122, "reference_start": 1, "reference_end": 122, "reference_sequence": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVWKGMTMPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK" }, "paralog": false, "identity": 0.9910313901345291, "score": 427.0, "evalue": 2.24e-154, "description": "Internal stop codon at 389427, 389526. Nonsense mutation around 389427, 389526.", "psc": { "uniref90_id": "UniRef90_D1J886", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J886", "UniRef:UniRef50_Q98PY0", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "DFOKDJOJIG_701", "locus": "GCA013367855_00687" }, { "type": "cds", "sequence": "contig_1", "start": 389962, "stop": 390270, "strand": "-", "frame": 1, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855575.1", "SO:0001217", "UniParc:UPI0001BC22A6", "UniRef:UniRef100_D1J887", "UniRef:UniRef50_D1J887", "UniRef:UniRef90_D1J887" ], "nt": "ATGAAACTAGAAGTCAAAATTAAATCTTTTGATGCCCACTTAGTAGATGAAGCTGCTAAAAAAATAGTTTTATTAGCTCAGGCTGAAAAAGCAACTATTAGTGGTCCAATTCCTTTACCAACACATAAAGAAATTATTACCATCTTAAGATCTGTTCACGTTAACAAAAAATCTCGTGAACAATTTGAAAGCAGAACTCATAAACGTTTAATCGTTTTAGATAAAGTTTCTGAATCATTAGTTGATAAAGCTAAGAGATTAGATTTATCTGCTGGTGTACAAATAAGTTTAAAAACTGTTAATCAATAA", "aa": "MKLEVKIKSFDAHLVDEAAKKIVLLAQAEKATISGPIPLPTHKEIITILRSVHVNKKSREQFESRTHKRLIVLDKVSESLVDKAKRLDLSAGVQISLKTVNQ", "aa_hexdigest": "f6c7d2f92638f7c729b3f6b0f0f4476d", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A6", "ncbi_nrp_id": "WP_012855575.1", "uniref100_id": "UniRef100_D1J887", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A6", "RefSeq:WP_012855575.1", "UniRef:UniRef100_D1J887" ] }, "ips": { "uniref100_id": "UniRef100_D1J887", "uniref90_id": "UniRef90_D1J887" }, "psc": { "uniref90_id": "UniRef90_D1J887", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_D1J887", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_D1J887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J887" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "DFOKDJOJIG_702", "locus": "GCA013367855_00688" }, { "type": "cds", "sequence": "contig_1", "start": 391482, "stop": 391589, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTGTATTTATATACAAATTCTTTGTCCAGATATGATTAAAAACAATTTAATGGGTAAAGAATCGGAAGGATATATCTACTTAGATCTTTCGGATGCTGATTAA", "aa": "MPCIYIQILCPDMIKNNLMGKESEGYIYLDLSDAD", "aa_hexdigest": "9511f5b3800971f3e26ddc174191068a", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4010.6492000000017, "isoelectric_point": 4.1011266708374015 }, "id": "DFOKDJOJIG_703", "locus": "GCA013367855_00689" }, { "type": "cds", "sequence": "contig_1", "start": 391638, "stop": 391910, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D20EF", "UniRef:UniRef90_UPI00215D20EF" ], "nt": "ATGTTTCAAAGAAAGCTATTATCCGCTTCTCAAATTTCAGAAATAACAACACAATTTTCATTTGAACTTTCCAAAGAAGGCAAAAAATTTGTAGCCAACAAAGGAGCCAAAGCCTTAATTGAAATTGTTAATAAGGCAATTGATGAAACTAAAAAATACGACACTATTGAAATGCTAAAAAGTCAAGAAAGAATTGCTTATACATATTTTATGGAAGCTAATGACATTAAAAAATATTTTGATATAAAAATTCCTGAAAATCTTATGCCTTGA", "aa": "MFQRKLLSASQISEITTQFSFELSKEGKKFVANKGAKALIEIVNKAIDETKKYDTIEMLKSQERIAYTYFMEANDIKKYFDIKIPENLMP", "aa_hexdigest": "423ab542b806597d496d80a3aa258fc1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 391482, "stop": 392054, "observations": { "mutation": [ 391640 ], "stop": [ 391640 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D20EF", "query_cov": 1.0, "subject_cov": 0.47368421052631576, "identity": 0.978, "score": 169.0, "evalue": 4.92e-52, "gene_start": 1, "gene_end": 90, "reference_start": 49, "reference_end": 138, "reference_sequence": "GTTDTNNKNIDSTNTGTADTNDKKDDNKDTNNENTDDTDPVQPDRLHRMFQRKLLSASQISEITTQFSFELSKEGKKFVANNGAKALIEIVNKAIDETKKYDTIEMLKSQERIAYTYFMEADDIKKYFDIKIPENLMPWGHKLLIEFLKKGEEKNMPCIYIQILCPDMIKNNLMGKESEGYIYLDLSDAD" }, "paralog": false, "identity": 0.9368421052631579, "score": 311.0, "evalue": 3.66e-110, "description": "Internal stop codon at 391640. Nonsense mutation around 391640.", "psc": { "uniref90_id": "UniRef90_UPI00215D20EF", "uniref50_id": "UniRef50_UPI00215D20EF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D20EF", "UniRef:UniRef50_UPI00215D20EF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D20EF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D20EF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10491.094199999994, "isoelectric_point": 8.802593803405763 }, "id": "DFOKDJOJIG_704", "locus": "GCA013367855_00690" }, { "type": "cds", "sequence": "contig_1", "start": 391865, "stop": 392281, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAATATTTTATTAAGTTTTATTTTTCCAGCTGTGTCATTATTACCTCTTTTAAGCATATCTTGCAAGGAAACTACCACAATTAATAATACCTTAGATACAAGTAATAACACTATTGATATAGATAATAGTGGCACTACAGATACTAATGGCAAAAATACAGATAGTACAAATACTGGTACCACTAATACTGATAATAAGAATATAGGTATTCTAGTGTTGGCACTACAGATACTAATGACAAAAATACAGACAGCACAAATACCGGTACCGCCGATACTAACGACAAGAAAGATGACAATAAAGATACAAATAACGAAAATACTGATGATACAGATCCTGTACAACCCGATAGACTTCATAGAATGTTTCAAAGAAAGCTATTATCCGCTTCTCAAATTTCAGAAATAA", "aa": "MKKNILLSFIFPAVSLLPLLSISCKETTTINNTLDTSNNTIDIDNSGTTDTNGKNTDSTNTGTTNTDNKNIGILVLALQILMTKIQTAQIPVPPILTTRKMTIKIQITKILMIQILYNPIDFIECFKESYYPLLKFQK", "aa_hexdigest": "b5e0b54c5e61f8bf2a80888518ea78d8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15425.901999999985, "isoelectric_point": 8.90638790130615 }, "id": "DFOKDJOJIG_705", "locus": "GCA013367855_00691" }, { "type": "cds", "sequence": "contig_1", "start": 392714, "stop": 393031, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATATTGAAGGTCCCGGACATGGTGGACAAGTTATGATTTCAAACTCTTATCTTGATGGATCTTATTCAGAAATATATCCTGAAATATCTCAAGATGAAGCCGGACTTGCAAAAATGTTCAAAAGATTCTCATTCCCTGGTGGAACTGCAAGCCATGCAGCGCCTGAAACACCTGGTTCAATTCATGAAGGTGGTGAATTAGGATATTCTATAAGCCATGGAACAGGAGCAATATTAGATAACCCTGATGTTATTTGTGCAGCTGTTGTTGGCGATGGTGAAGCAGAAACAGGCCCACTAGCAACTAGCTGA", "aa": "MFYIEGPGHGGQVMISNSYLDGSYSEIYPEISQDEAGLAKMFKRFSFPGGTASHAAPETPGSIHEGGELGYSISHGTGAILDNPDVICAAVVGDGEAETGPLATS", "aa_hexdigest": "cb9a70a1207895298182aa6f7a6c75c0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10793.721599999992, "isoelectric_point": 4.3166601181030275 }, "id": "DFOKDJOJIG_706", "locus": "GCA013367855_00692" }, { "type": "cds", "sequence": "contig_1", "start": 392902, "stop": 393222, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGAGATTACTTTTATCTTCGCTTCATTCAACGAAAATTGGATTTCAGCCTAAACCTTCAAAGTATTTTTTAATTTCTTCTTTTGGTTTTCTTGAAAGAAGTGTAGGGTTTGAAATTTTTCCACCATTCAAATGTAATATAGGAAGTATTGCTCCATCATTTACAGGGTTAATAAAAGCATTTGAGAATCAGCTAGTTGCTAGTGGGCCTGTTTCTGCTTCACCATCGCCAACAACAGCTGCACAAATAACATCAGGGTTATCTAATATTGCTCCTGTTCCATGGCTTATAGAATATCCTAATTCACCACCTTCATGA", "aa": "MSRLLLSSLHSTKIGFQPKPSKYFLISSFGFLERSVGFEIFPPFKCNIGSIAPSFTGLIKAFENQLVASGPVSASPSPTTAAQITSGLSNIAPVPWLIEYPNSPPS", "aa_hexdigest": "e74dad0e4e0293078566f4399dcc17f9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11315.932199999985, "isoelectric_point": 9.359794044494631 }, "id": "DFOKDJOJIG_707", "locus": "GCA013367855_00693" }, { "type": "cds", "sequence": "contig_1", "start": 393233, "stop": 393328, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAATCATTAGATAAGGCTATTGAATCAATTAAAGAAATTCAAGCAGAAGCAAGAAAAAAACCAGCCGAAGAAGCAACAAGACCAACTTGA", "aa": "MAKSLDKAIESIKEIQAEARKKPAEEATRPT", "aa_hexdigest": "9dabdcbb4dcd8d8b3e81f6139aafeee5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3440.9214000000006, "isoelectric_point": 8.141083717346195 }, "id": "DFOKDJOJIG_708", "locus": "GCA013367855_00694" }, { "type": "cds", "sequence": "contig_1", "start": 393680, "stop": 393808, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAGATGGCAAAATTTGCTGCTGATATAATGAAAGATAACCCTTCATCATTTAGAGTATTTGGACCTGATGAAACTAAATCAAATCGTATGTTTGCATTATTCAATGTAACAAATAGACAATGA", "aa": "MAEMAKFAADIMKDNPSSFRVFGPDETKSNRMFALFNVTNRQ", "aa_hexdigest": "7538684bd81affb55bcf87471adf19fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4814.4602, "isoelectric_point": 8.253452110290528 }, "id": "DFOKDJOJIG_709", "locus": "GCA013367855_00695" }, { "type": "cds", "sequence": "contig_1", "start": 394196, "stop": 394324, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAGCCTTAACCGAAAGAAATGTAATTAACTTAATTGTTGCTTCAAAACAACCAAGAGAACAATTCTTTACAGTTGAAGATGCTGAAGAATTACTAGAAAAAGGTTATAAGGTTGTTCCTTGA", "aa": "MNKALTERNVINLIVASKQPREQFFTVEDAEELLEKGYKVVP", "aa_hexdigest": "06265444dd5f8e3c2b7c8e8889e02782", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4848.5314, "isoelectric_point": 5.189195442199705 }, "id": "DFOKDJOJIG_710", "locus": "GCA013367855_00696" }, { "type": "cds", "sequence": "contig_1", "start": 394400, "stop": 394840, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGCTATTTCACTAATTAACCAAGAATATCCACATCTAAAAATAAGATATGTATATGTTCTTGACTTGTTAAAATTGAGATCTAGAAAAATAGATCCAAGAGGAATTAGCGATGAAGAATTTGATAAGGTATTTACTAAAAATAAACCTATCATATTTGCTTTCCATGGATTTGAAGGGTTATTAAGAGACATCTTCTTTACAAGATCTAACCACAATTTAATTGCACACGGATATCGTGAAAACGGTGATATTACAACATCATTCGATATTAGACAATTAAGTGAAATGGATAGATATCATATTGCTAAAGATGCAGCAGAAGCAGTATATGGAAAAGATGCAAAAGCATTTATGAATAAATTAGACCAAAAACTAGAATATCATAGAAACTACATTGATGAATATGGATATGATATGCCAGAAGTTGTTGAATGA", "aa": "MAAISLINQEYPHLKIRYVYVLDLLKLRSRKIDPRGISDEEFDKVFTKNKPIIFAFHGFEGLLRDIFFTRSNHNLIAHGYRENGDITTSFDIRQLSEMDRYHIAKDAAEAVYGKDAKAFMNKLDQKLEYHRNYIDEYGYDMPEVVE", "aa_hexdigest": "59f58ddc36c55ba5c33ce12997ad2aed", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA37XNB4", "query_cov": 0.9863013698630136, "subject_cov": 0.8674698795180723, "identity": 0.59, "score": 189.0, "evalue": 3.88e-59, "valid": false, "product": "Xylulose 5-phosphate/Fructose 6-phosphate phosphoketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A3B0NYC0" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17242.383899999986, "isoelectric_point": 6.071961784362792 }, "id": "DFOKDJOJIG_711", "locus": "GCA013367855_00697" }, { "type": "cds", "sequence": "contig_1", "start": 394947, "stop": 395162, "strand": "+", "frame": 3, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855578.1", "SO:0001217", "UniParc:UPI0001BC22A9", "UniRef:UniRef100_D1J890", "UniRef:UniRef50_Q6KIG2", "UniRef:UniRef90_D1J890" ], "nt": "ATGCAAAAAGATATTCACCCAAAATACAACCAAATTAAAGCTGTTTGCTCATCATGCAATTCAACAATAGAATTTGGTTCAACTGCTGATAAAGTTACTTTAGATGTTTGCTCTAATTGTCATGCTTTCTACACAGGAAACCGTACAGATGTTAAAGCTCGTGGACGTGTTGAAAGATTTAATAAGATATTAGAAAAATCACATAAAAAGAACTAA", "aa": "MQKDIHPKYNQIKAVCSSCNSTIEFGSTADKVTLDVCSNCHAFYTGNRTDVKARGRVERFNKILEKSHKKN", "aa_hexdigest": "14ec0f3da0d8b604325a5ed87f9f7f2a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A9", "ncbi_nrp_id": "WP_012855578.1", "uniref100_id": "UniRef100_D1J890", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A9", "RefSeq:WP_012855578.1", "UniRef:UniRef100_D1J890" ] }, "ips": { "uniref100_id": "UniRef100_D1J890", "uniref90_id": "UniRef90_D1J890" }, "psc": { "uniref90_id": "UniRef90_D1J890", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_Q6KIG2", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG2" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "DFOKDJOJIG_712", "locus": "GCA013367855_00698" }, { "type": "cds", "sequence": "contig_1", "start": 395257, "stop": 395496, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J891", "UniRef:UniRef90_D1J891" ], "nt": "ATGGAATATTTGTTATATTTGAAATTGAAGGGAGAAATAAAAATGAATATAAATTTCAAACTAATGAGCGAAGAAGAAACAAAAAATATTGTAGGAGGTTTTGCAATTACTGCTTTAATTTCAACTATTTTGTCTGTAGTAACAAGCACTGTCGGAATTATTGGAACTTCAATAGGCTTGGCTAAGACTTTAAATGCAAATAAAGGAGAAGTAAAAGCCGGGAATATTTCATTGAAATGA", "aa": "MEYLLYLKLKGEIKMNINFKLMSEEETKNIVGGFAITALISTILSVVTSTVGIIGTSIGLAKTLNANKGEVKAGNISLK", "aa_hexdigest": "5ae79da8f2d0aaa4e27df05c8fffdaa5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J891", "query_cov": 1.0, "subject_cov": 0.8144329896907216, "identity": 0.987, "score": 140.0, "evalue": 4.64e-42, "valid": true, "uniref50_id": "UniRef50_D1J891" }, "pscc": { "uniref50_id": "UniRef50_D1J891", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J891" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8445.945599999995, "isoelectric_point": 9.347996330261232 }, "id": "DFOKDJOJIG_713", "locus": "GCA013367855_00699" }, { "type": "cds", "sequence": "contig_1", "start": 395569, "stop": 395889, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002358B08E", "UniRef:UniRef90_UPI001319C858" ], "nt": "ATGCTCTTGCCATTTGCTAATATTGCTAATAAATCTAAAAATATTGCATATTTAATCTCATTGGGTCTTCAACAAATGTTATATTCATTCTTTATCACTATTTTCTTTAGTAATACTTATTTCTTATTTGATAAGCAAAAATTTAAAGTCAATACTATTTTTTATACACTAATATTTAGAATTGTTAATTCTTCAATAATTCTTATTGTATTGACTTTGATAAATAACTTTGTAAACTATTTTGTAACCTTTGCAGTATATTCATTACTAATATTAATATGTAGCATAACAATAATCTTTGCGAACGAAAAATTAATTTAA", "aa": "MLLPFANIANKSKNIAYLISLGLQQMLYSFFITIFFSNTYFLFDKQKFKVNTIFYTLIFRIVNSSIILIVLTLINNFVNYFVTFAVYSLLILICSITIIFANEKLI", "aa_hexdigest": "ef5d4e6832c5b3326d2ed7bc92726b05", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001319C858", "query_cov": 0.9811320754716981, "subject_cov": 1.0, "identity": 0.99, "score": 185.0, "evalue": 5.06e-59, "valid": true, "uniref50_id": "UniRef50_UPI002358B08E" }, "pscc": { "uniref50_id": "UniRef50_UPI002358B08E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002358B08E" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12360.745599999982, "isoelectric_point": 9.56319179534912 }, "id": "DFOKDJOJIG_714", "locus": "GCA013367855_00700" }, { "type": "cds", "sequence": "contig_1", "start": 396919, "stop": 397335, "strand": "-", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE0DEE4", "UniRef:UniRef90_UPI002FE0DEE4" ], "nt": "ATGCTTGGTGGAATTAGTGACTTTGATTTTAAAGAAATTACGCCAACTTCAATTCATGCTTTAATGTTAGAAAAATTGCATAATCTAGAAGCAAATATATTATTGAATTACGAACAATATAGATTTGTTAATGTTATTAAGGATATTAATAATTTTGTTATTGATTTAAGTAGTTACTATATTTCTATAACCAAAGATATTTTATATCTTGAAAAACAAGATGACCCTGAACGTAGAATGGTTCAATATGTTTTTAGCAACATTATTGAGGTCTTATTAAAAGCTCTTGCCCCAATATTGCCAACTACAACCGAAGAAATTTATAAATATTACAATAAACCTAACAAGTTGCCATCCGTTCATTTATTGTCATTTTTAGAAAGAAAAGATTTTGATTTTAAATTACAAGATCAATGA", "aa": "MLGGISDFDFKEITPTSIHALMLEKLHNLEANILLNYEQYRFVNVIKDINNFVIDLSSYYISITKDILYLEKQDDPERRMVQYVFSNIIEVLLKALAPILPTTTEEIYKYYNKPNKLPSVHLLSFLERKDFDFKLQDQ", "aa_hexdigest": "65a5842e059e383051f0ed8641b6a9d7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 396811, "stop": 397326, "observations": { "mutation": [ 396831, 396921 ], "stop": [ 396831, 396921 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE0DEE4", "query_cov": 0.9782608695652174, "subject_cov": 0.7627118644067796, "identity": 0.993, "score": 268.0, "evalue": 1.72e-90, "gene_start": 4, "gene_end": 138, "reference_start": 3, "reference_end": 137, "reference_sequence": "MLGISDFDFKEITPTSIHALMLEKLHNLEANILLNYEQYRFVNVIKDINNFVIDLSSYYISITKDILYLEKQDDPERRMVQYVFSNIIEVLLKALAPILPTTTEEIYKYYNKPNKLPSVHLLSFLERKDFDFKFQDQWKEFFEIKDEVYKLIEEKIKSQAIKRPNEAWVVIKAIQTL" }, "paralog": false, "identity": 0.9766081871345029, "score": 328.0, "evalue": 1.95e-116, "description": "Internal stop codon at 396831, 396921. Nonsense mutation around 396831, 396921.", "psc": { "uniref90_id": "UniRef90_UPI002FE0DEE4", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI002FE0DEE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE0DEE4", "UniRef:UniRef50_UPI002FE0DEE4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE0DEE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE0DEE4" ], "product": "class I tRNA ligase family protein" } }, "id": "DFOKDJOJIG_715", "locus": "GCA013367855_00701" }, { "type": "cds", "sequence": "contig_1", "start": 397435, "stop": 397554, "strand": "-", "frame": 1, "gene": null, "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBK0", "UniRef:UniRef90_A0A432FBK0" ], "nt": "TTGGTTTCTCATGGGTTTGTTTTAGATGGCAAAGGCCAAAAAATGTCAAAATCAAAAGGCAATGTTGTTGATCCTTTAGCTGTAATTGATAAATATGGAGCAGATATTTTAAGGCTTTGA", "aa": "MVSHGFVLDGKGQKMSKSKGNVVDPLAVIDKYGADILRL", "aa_hexdigest": "3e3c4202b6a38a44c5865b9dd4e3e816", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 397435, "stop": 397596, "observations": { "mutation": [ 397581 ], "stop": [ 397581 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A432FBK0", "query_cov": 1.0, "subject_cov": 0.7358490566037735, "identity": 0.846, "score": 70.5, "evalue": 2.13e-15, "gene_start": 1, "gene_end": 39, "reference_start": 15, "reference_end": 53, "reference_sequence": "MNSIAYRGKSPYKNLVSHGFVLDGKNQKMSKSKGNVVDPLKIISKQGADILRL" }, "paralog": false, "identity": 0.7735849056603774, "score": 83.6, "evalue": 1.84e-23, "description": "Internal stop codon at 397581. Nonsense mutation around 397581.", "psc": { "uniref90_id": "UniRef90_A0A432FBK0", "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein", "uniref50_id": "UniRef50_A0A432FBK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A432FBK0", "UniRef:UniRef50_A0A432FBK0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FBK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBK0" ], "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein" } }, "id": "DFOKDJOJIG_716", "locus": "GCA013367855_00702" }, { "type": "cds", "sequence": "contig_1", "start": 398083, "stop": 398361, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGCCAGTTGTTTTTGGTCATCATGTTAGTTTAGATACTGGTACTGGCTTAGTTCACATTGCTCCATTATTTGGTGAAGATGACTTTATTATCGGCAAAAAAGAAAACCTAGAAATGATTATGCACATAAACGATGATGGAACAATCAATGATAAAGGCTTTGAATATAGCGGTGTTTTTTATGATGATGCAAATCCATTAATTGGCAAATTTTTAGCAGAAAATAATCAATTAATTGCGTTTAAGAAAATTAAACACTCATACCCTCATGATTGA", "aa": "MGPVVFGHHVSLDTGTGLVHIAPLFGEDDFIIGKKENLEMIMHINDDGTINDKGFEYSGVFYDDANPLIGKFLAENNQLIAFKKIKHSYPHD", "aa_hexdigest": "ec0cfcc482567d5921c86253b942139e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10259.48839999999, "isoelectric_point": 5.131447029113769 }, "id": "DFOKDJOJIG_717", "locus": "GCA013367855_00703" }, { "type": "cds", "sequence": "contig_1", "start": 398440, "stop": 398562, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGGCTAACTCTGGCGTTGCAATTAACGATCAATTTGAATATGCAAAAGTGTTAGTAAATGGAAACAAATATATAATTGCAAAAGATTTAGTTGAATCAGTTGCTGCAGTTGCTAAATGA", "aa": "MLANSGVAINDQFEYAKVLVNGNKYIIAKDLVESVAAVAK", "aa_hexdigest": "31f4ca199c84f3b0ae8203bebb5441e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4267.897200000002, "isoelectric_point": 5.970674705505371 }, "id": "DFOKDJOJIG_718", "locus": "GCA013367855_00704" }, { "type": "cds", "sequence": "contig_1", "start": 398716, "stop": 398979, "strand": "-", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AA8A25", "UniRef:UniRef90_UPI0023AA8A25" ], "nt": "TTGCCTATTGAACATAAAATGTTGCTAGAAGCTAAAAAGAATGCTAAAGATTTTAGTGTTATTGAATTAAGAAAGAAGGCAGCAGAATATGCTTTAAGTCAAGTTGAACATCAAAAGGCTCAATTTAAGAAATTAAGTTTATTGACAGACTTTTCAGAAATTTATGTGACATTGGATAAAAACTTAGAATTTAAGCAATTAGAACTTTTTGCTAAAATGGTTAAAGAAGGCCTAATTTATCGCGATTTGAAACCAGTTTATTGA", "aa": "MPIEHKMLLEAKKNAKDFSVIELRKKAAEYALSQVEHQKAQFKKLSLLTDFSEIYVTLDKNLEFKQLELFAKMVKEGLIYRDLKPVY", "aa_hexdigest": "3a4646ba2f4069d42af0db44b32f3f27", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 398413, "stop": 398979, "observations": { "mutation": [ 398442, 398568, 398583, 398718 ], "stop": [ 398442, 398568, 398583, 398718 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AA8A25", "query_cov": 1.0, "subject_cov": 0.4484536082474227, "identity": 0.9890000000000001, "score": 166.0, "evalue": 1.62e-50, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MPIEHKMLLEAKKNAKDFSVIELRKKAAEYALSQVEHQKAQFKKLSLFTDFSEIYVTLDKNLEFKQLELFAKMVKEGLIYRDLKPVYWSPSSQSALAEAEVEYADHVSPSLYVAFKVIEGNKYVENGDYLIIWTTTPWTLLANSGVAINDQFEYAKVLVNGNKYIIAKDLVESVAAVAKWEEYQVLSTFKGKEF" }, "paralog": false, "identity": 0.9627659574468085, "score": 345.0, "evalue": 1.09e-123, "description": "Internal stop codon at 398442, 398568, 398583, 398718. Nonsense mutation around 398442, 398568, 398583, 398718.", "psc": { "uniref90_id": "UniRef90_UPI0023AA8A25", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI0023AA8A25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AA8A25", "UniRef:UniRef50_UPI0023AA8A25" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AA8A25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AA8A25" ], "product": "class I tRNA ligase family protein" } }, "id": "DFOKDJOJIG_719", "locus": "GCA013367855_00705" }, { "type": "cds", "sequence": "contig_1", "start": 398992, "stop": 399285, "strand": "-", "frame": 1, "gene": null, "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352TTR4" ], "nt": "ATGGAAGAAAAGAAAGATTATAAAGACACGTTATTGATGCCAAATACTGATTTTCCAATGAAAGCAAATTTAGTTGAAAAAGAATTGGAATATCAAAAACAATGGCTAGATATTAAGTTGTATCAAACTATTTTAAATAGAAATAAAAACAGTCAATCATTTGTATTGCATGATGGTCCACCATATGCAAATGGTAATATTCATGTTGGACACGCTCTAAACAAAATTTTAAAAGATATAATTGTAAGATTTAGAAGTATGCAAGGATATTATTCGCCATATGTTCCGGGTTGA", "aa": "MEEKKDYKDTLLMPNTDFPMKANLVEKELEYQKQWLDIKLYQTILNRNKNSQSFVLHDGPPYANGNIHVGHALNKILKDIIVRFRSMQGYYSPYVPG", "aa_hexdigest": "68b231c91ac2e4767637ea05b37ce65a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00190B9126", "query_cov": 0.9381443298969072, "subject_cov": 0.9891304347826086, "identity": 0.626, "score": 129.0, "evalue": 2.2e-37, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A352TTR4" }, "pscc": { "uniref50_id": "UniRef50_A0A352TTR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352TTR4" ], "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_720", "locus": "GCA013367855_00706" }, { "type": "cds", "sequence": "contig_1", "start": 399529, "stop": 399744, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "TTGATTTTAGAAGTTAGCGAAAATATTGAAAAAACTAAGTTTAATATTGCAATAAGCAAAATGATGGTGTTTACTAATTACTTAACATCTTTAAAAACCATTCCATCATCATTTGTTTTAAAACAATTTGCTATTTTATTAAGTCCGTTTGCGCCATTTTTAGCTGAAGAACTTTTGTTTATGTTGAATGAAAAACCAATTCAATATCAAACTTGA", "aa": "MILEVSENIEKTKFNIAISKMMVFTNYLTSLKTIPSSFVLKQFAILLSPFAPFLAEELLFMLNEKPIQYQT", "aa_hexdigest": "b5d80819d9f4ac2c827be821519144af", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 85, "aa_cov": 0.9014084507042254, "hmm_cov": 1.0, "evalue": 1.9777672544229314e-10, "score": 36.3955192565918, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 8205.705999999995, "isoelectric_point": 6.140395927429199 }, "id": "DFOKDJOJIG_721", "locus": "GCA013367855_00707" }, { "type": "cds", "sequence": "contig_1", "start": 399871, "stop": 400008, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGGGACCTGATGGTCAAAAAATGTCTAAATCTCTAGGTAATGTTATAAATCCTGATGATTTTATTTTATCTCATGGAGCAGATAGTTTAAGAGTTTATGAAATGTTTATGGGGCCTTTAGTGGAATCAAAATCTTGA", "aa": "MGPDGQKMSKSLGNVINPDDFILSHGADSLRVYEMFMGPLVESKS", "aa_hexdigest": "ff65653323f4eab9a9bfb6e0a6263c3e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 30, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 1.6304900271505442e-10, "score": 35.888710021972656, "start": 4, "stop": 33 } ], "seq_stats": { "molecular_weight": 4899.556399999999, "isoelectric_point": 4.889426612854003 }, "id": "DFOKDJOJIG_722", "locus": "GCA013367855_00708" }, { "type": "cds", "sequence": "contig_1", "start": 400474, "stop": 400728, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "ATGTCATATGGAAGTGGGGCAGTAATGTGTGTGCCTGGCCATGATCAAAGAGATTATGAATTTGCTAAAAAATTTGATTTAAAAATTAAAAAAATTATTGAATGTGATGAATTACCATTTTTAGGAGATGGTATTCATATCAATTCAGATATTGCAAATGGTTTAGACAACACAGGCGCAGTAAAATGTATTTTTAATGAATTGAAAGCAAATAATTTGGCAGATGAAAAAGTAAGTTATAAATTACGCGATTGA", "aa": "MSYGSGAVMCVPGHDQRDYEFAKKFDLKIKKIIECDELPFLGDGIHINSDIANGLDNTGAVKCIFNELKANNLADEKVSYKLRD", "aa_hexdigest": "85ce95745926eaef7746ab3184eb7dd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 77, "aa_cov": 0.8095238095238095, "hmm_cov": 1.0, "evalue": 5.19391170436731e-19, "score": 64.08448028564453, "start": 1, "stop": 68 } ], "seq_stats": { "molecular_weight": 9352.553299999992, "isoelectric_point": 5.265132331848144 }, "id": "DFOKDJOJIG_723", "locus": "GCA013367855_00709" }, { "type": "cds", "sequence": "contig_1", "start": 400747, "stop": 400995, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "TTGCAACTCTCAACATTTACTACTAGATTGGATACTATCTATGGTGTAACTGCAATAGTAATTTCTCCCGAACACCCACTTCTTCAAAAAATAACTACCGCAAAATATCATGATGATATTTCTAACTATTTAAAAGAAGTTAAATTAAAAAATGAATTAGAAAGAACATCTTTAAATAAAGATAAAACAGGATATTTTACTGGTTCGTATGCAATTCATCCATTGACTAATGAATTAATTCCTATTTGA", "aa": "MQLSTFTTRLDTIYGVTAIVISPEHPLLQKITTAKYHDDISNYLKEVKLKNELERTSLNKDKTGYFTGSYAIHPLTNELIPI", "aa_hexdigest": "191f04c27562310af5aaf71b65e975e4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 81, "aa_cov": 0.9878048780487805, "hmm_cov": 0.9753086419753086, "evalue": 3.512456527693662e-24, "score": 80.93769073486328, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 9353.616299999994, "isoelectric_point": 6.8211565017700195 }, "id": "DFOKDJOJIG_724", "locus": "GCA013367855_00710" }, { "type": "cds", "sequence": "contig_1", "start": 401497, "stop": 401619, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCTTACCCTTCGGGAATGGGATTGCACGTTGGGCATTTAGAAGGTTATACCGCTACTGATATTATTGCTAGGTATAAACGTCTAAATGGTTTTGATGTGTTGCATCCTATTGGTTGA", "aa": "MFPYPSGMGLHVGHLEGYTATDIIARYKRLNGFDVLHPIG", "aa_hexdigest": "21af7fc6286f9ee63374d2bcb75ef168", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4445.087, "isoelectric_point": 6.810016059875489 }, "id": "DFOKDJOJIG_725", "locus": "GCA013367855_00711" }, { "type": "cds", "sequence": "contig_1", "start": 402002, "stop": 403243, "strand": "+", "frame": 2, "gene": null, "product": "Lmp protein", "db_xrefs": [ "RefSeq:WP_175972279.1", "SO:0001217", "UniParc:UPI001588F879", "UniRef:UniRef100_UPI001588F879", "UniRef:UniRef50_A0A454CAH5", "UniRef:UniRef90_A0A454CAH5" ], "nt": "ATGAAAAGAAAAATTTTACTATTTACCCCCTTAATTATTGCTGGTAGCTTAATACCAACAACATTGACAGCATGCAATAATGAAGATAGCAAACTTACAAAGCAATTAAAAGCAAAGCAATTAAAAGCAAAGCAAACATTTAATAATTTAAAAAATAAATTGCAAGATTTATTGAACTCAGAAGATGCAAAAGATATTGATGCAAATAAAGAAATTAATACACTTGCAAACACAACAATTGATGATAATTCTCAAATACAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTGACTAAGAAAATTAATGATAAAAAAATCCTAATAAAACTAAGACAAGATTTCAACAAAGCAAAAGAAGATCTAAGAAATCTTTTGGCGTCAAAAGAAGTGGCGGATATTGAAAAAGAAATCGAATTAAACACTCTAAATACCACTGATATTGCCAAAGATTCTTTACAACAAGAAATTCAAACCAAAATACAAAAAATTCAAGAAGCTATACAATCAATAAACAAAAAAATCAATGACAAAAAGCTATTAAACAAAGAACAACAAAATCTTGTTGAAGCAAAAAAGACACTTGAAAATTTAATTGCTACAGAAGATGCAAAAACAGTCGGAATTGATGAAGCAAAAAAAATTCTTGACAATAATAAAAATAATAAAATATCAACTATTGAAGAAATTAAAAAAGCAATAAACGATTTGAATGATATTACAGCCAAATTAAATCAAAAAATTGCAAACAAAAAGCAAGAATTATTAGAAAATTTCAAAAAATCTAAACAAGATTTAGAAGAATACATAAATAAAAATTTAAAAGAAATCAGATTTATTGAAGTTAAAGAAAAAGCCCAAAAAACCATTGAAAAATTTTCTAATATAAATAAAGATAATTCCTCAATCAAAGAAATTACAGATGCTACTAAATCGCTAAATAAAGCCAAAGAAGATGCTTTAAAAGAAGAAGAAGGAATCAAAAAATTAAACGAAGTCTTGGAATATGTCAATAAAAGAAATGAACTAGAAAAATTAATAAAATCATCTAATACTCAATCAAATGAAGATGCTATTAAAAAAGCCCAAAAAACTTTAGATGATAATAAAATAGCAAATACCAACGAATCATCTTCAAAAGATTTTGAAGAAAAAAGAAAAAATATTGAAAAAGCAATAATTGAACTAAAGGCAGCAATAGAAGCAACAAAAAAATCTAAGAATTAA", "aa": "MKRKILLFTPLIIAGSLIPTTLTACNNEDSKLTKQLKAKQLKAKQTFNNLKNKLQDLLNSEDAKDIDANKEINTLANTTIDDNSQIQDIESKTKTIEDAIESLTKKINDKKILIKLRQDFNKAKEDLRNLLASKEVADIEKEIELNTLNTTDIAKDSLQQEIQTKIQKIQEAIQSINKKINDKKLLNKEQQNLVEAKKTLENLIATEDAKTVGIDEAKKILDNNKNNKISTIEEIKKAINDLNDITAKLNQKIANKKQELLENFKKSKQDLEEYINKNLKEIRFIEVKEKAQKTIEKFSNINKDNSSIKEITDATKSLNKAKEDALKEEEGIKKLNEVLEYVNKRNELEKLIKSSNTQSNEDAIKKAQKTLDDNKIANTNESSSKDFEEKRKNIEKAIIELKAAIEATKKSKN", "aa_hexdigest": "c69e8c3381e86875cab0b69e492ac6c1", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI001588F879", "ncbi_nrp_id": "WP_175972279.1", "uniref100_id": "UniRef100_UPI001588F879", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588F879", "RefSeq:WP_175972279.1", "UniRef:UniRef100_UPI001588F879" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588F879", "uniref90_id": "UniRef90_A0A454CAH5" }, "psc": { "uniref90_id": "UniRef90_A0A454CAH5", "product": "Lmp protein", "uniref50_id": "UniRef50_A0A454CAH5" }, "pscc": { "uniref50_id": "UniRef50_A0A454CAH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAH5" ], "product": "Lmp protein" }, "genes": [], "id": "DFOKDJOJIG_726", "locus": "GCA013367855_00712" }, { "type": "cds", "sequence": "contig_1", "start": 403516, "stop": 403626, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAGAAGAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGA", "aa": "MIRREYQKGFNFNILTQEDLDTTIAKINNMPREILN", "aa_hexdigest": "8ef7b9776e982e4522979ce89f78742f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4340.9349, "isoelectric_point": 6.038881492614746 }, "id": "DFOKDJOJIG_727", "locus": "GCA013367855_00713" }, { "type": "cds", "sequence": "contig_1", "start": 403633, "stop": 403914, "strand": "-", "frame": 1, "gene": null, "product": "IS30 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "nt": "TTGGTTCTTGGAAAAAGAGCAAATGGCTATAAAAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGCGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGA", "aa": "MVLGKRANGYKNILTLTERKTRVGFATFVSSKSPFEINSKIKELIKNKNLIVKTITIDNGIEFEKIGFLAKSLNIIIYRAEPYASFQRGSNEN", "aa_hexdigest": "151ece11318bfe9f9904904a28af0e1a", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001F20DEBE", "query_cov": 0.978494623655914, "subject_cov": 0.8272727272727273, "identity": 0.67, "score": 125.0, "evalue": 1.07e-35, "valid": false, "product": "IS30 family transposase", "uniref50_id": "UniRef50_UPI001F20DEBE", "go_ids": [ "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F20DEBE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "product": "IS30 family transposase" }, "genes": [], "id": "DFOKDJOJIG_728", "locus": "GCA013367855_00714" }, { "type": "cds", "sequence": "contig_1", "start": 404098, "stop": 404472, "strand": "-", "frame": 1, "gene": null, "product": "Helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "nt": "ATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGTTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCGAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAGTTTTCCAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGA", "aa": "MNYNIHKYFHLSLEERFFIQRSLENNLSLRAISRQLNRSASTISREIKRNSNFNEYIFLRADKKSKFRAYHKYMFKFNINFEFEEFSKIFKSKYDKKYFGVKATMNYIEENFNVKKPTMRTVFN", "aa_hexdigest": "c57bd1123260a439ee4f69e485682c4a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0AAJ1UWR4", "query_cov": 1.0080645161290323, "subject_cov": 0.8116883116883117, "identity": 0.6, "score": 141.0, "evalue": 1.13e-40, "valid": false, "product": "Helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_A0AAJ1UWR4" }, "pscc": { "uniref50_id": "UniRef50_A0AAJ1UWR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "product": "Helix-turn-helix domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_729", "locus": "GCA013367855_00715" }, { "type": "cds", "sequence": "contig_1", "start": 404750, "stop": 405238, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D52A", "UniRef:UniRef90_UPI002410D52A" ], "nt": "ATGGGTAAAAAAGCAAAATTATTTGTCTTTGGTGGGATTAGTTTTTTAGTGGCGGCTTCAATTATTGGTTCTGTTGCAGCATATGCTTCAATCAATAAAAAGAATGAAATTAGAGTTAGCGAATATAATAAATTTCTTGAAGAAAAAACTAATTTAAATTCTTTTAGCAATTTAGAAAATGATAAAAAAATTAGTTCAATAAAATCTAATACCTTAAGTGAAGTTGAAAAGATAGATAAAAATTCAGATTTATTTACTATTCAAAAAGCCCGCAAAATAGTCAACGAATCCATTGAAAATATCAAAAATATAATAGCATCGCATAAAAAAATGTTATTAGAAAAAGAAAAAGCGTTTATTCAATATAACAATGTAAAAAAGCAATTGCAAGATATGCTTTTAAAAATTTTTGACTTTTTGAATAAAGAAAGCCAAGGACATTATTACATTGGCGATGGAAACGAAAAAATAATAAACAAACAAAATTGA", "aa": "MGKKAKLFVFGGISFLVAASIIGSVAAYASINKKNEIRVSEYNKFLEEKTNLNSFSNLENDKKISSIKSNTLSEVEKIDKNSDLFTIQKARKIVNESIENIKNIIASHKKMLLEKEKAFIQYNNVKKQLQDMLLKIFDFLNKESQGHYYIGDGNEKIINKQN", "aa_hexdigest": "f2d0209ccf6368c2ac76900e94be97f4", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 404750, "stop": 405404, "observations": { "insertion": [ 405359 ], "mutation": [ 405236 ], "stop": [ 405236 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410D52A", "query_cov": 1.0, "subject_cov": 0.7465437788018433, "identity": 1.0, "score": 298.0, "evalue": 3.35e-101, "gene_start": 1, "gene_end": 162, "reference_start": 1, "reference_end": 162, "reference_sequence": "MGKKAKLFVFGGISFLVAASIIGSVAAYASINKKNEIRVSEYNKFLEEKTNLNSFSNLENDKKISSIKSNTLSEVEKIDKNSDLFTIQKARKIVNESIENIKNIIASHKKMLLEKEKAFIQYNNVKKQLQDMLLKIFDFLNKESQGHYYIGDGNEKIINKQNWLLKEINENQKYFSNITIDSDIDSIKLATTKLVEIKFLINENKKHINLPKNITAY" }, "paralog": false, "identity": 0.9815668202764977, "score": 406.0, "evalue": 3.79e-146, "description": "Internal stop codon at 405236. Frameshift due to insertion around 405359. Nonsense mutation around 405236.", "psc": { "uniref90_id": "UniRef90_UPI002410D52A", "uniref50_id": "UniRef50_UPI002410D52A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410D52A", "UniRef:UniRef50_UPI002410D52A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410D52A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D52A" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18498.11099999999, "isoelectric_point": 9.483186531066895 }, "id": "DFOKDJOJIG_730", "locus": "GCA013367855_00716" }, { "type": "cds", "sequence": "contig_1", "start": 405581, "stop": 405709, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGACAAAACTGCACTTCTTGAGGAATTCGACATTGATTTTTTAGGATTCAAAATCAAAGATAAAAACTTCTATAAAAATTATGTTGAGTTATTGACACCATATAAAAAGGCTATTGATTTGTGA", "aa": "MYDKTALLEEFDIDFLGFKIKDKNFYKNYVELLTPYKKAIDL", "aa_hexdigest": "487c16589ec9d4d1c25f273a1cf13972", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5094.869100000001, "isoelectric_point": 5.086430549621581 }, "id": "DFOKDJOJIG_731", "locus": "GCA013367855_00717" }, { "type": "cds", "sequence": "contig_1", "start": 405773, "stop": 406003, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024112F2E", "UniRef:UniRef90_UPI0024112F2E" ], "nt": "TTGTATATTAATGGTTATACTATCTTTTTTAAACCTAAAAAAATATATGCTTTGATAAATCAACCTGAACTAGACAAAGAACTAACTGAAACTACTGATGATTATAAATTTAAACTAATGCTTATCCCAAAAGAAAATACTATAGAACATAATAAATTAATAGAAAATTTTAAAAACTATTGCGACGATTTAATGAATAGAAGCAAAAAATTAAAAAACGACAATCAATAA", "aa": "MYINGYTIFFKPKKIYALINQPELDKELTETTDDYKFKLMLIPKENTIEHNKLIENFKNYCDDLMNRSKKLKNDNQ", "aa_hexdigest": "d360ff4b85445756d6d1efd4595df859", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 405677, "stop": 406003, "observations": { "mutation": [ 405707, 405764 ], "stop": [ 405707, 405764 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024112F2E", "query_cov": 1.0, "subject_cov": 0.7037037037037037, "identity": 0.9740000000000001, "score": 154.0, "evalue": 2.63e-47, "gene_start": 1, "gene_end": 76, "reference_start": 33, "reference_end": 108, "reference_sequence": "MTPYKKAIDLWKNYYDSYSIGLKEVEEIIWDKLYINGYTIFFKPKKIYALINQPELDKELTETTDDYKFKLMLVPKENTIEHNKLIENFKNYCDDLMNRSKKLKNDNQ" }, "paralog": false, "identity": 0.9351851851851852, "score": 211.0, "evalue": 3.01e-72, "description": "Internal stop codon at 405707, 405764. Nonsense mutation around 405707, 405764.", "psc": { "uniref90_id": "UniRef90_UPI0024112F2E", "uniref50_id": "UniRef50_UPI0024112F2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024112F2E", "UniRef:UniRef50_UPI0024112F2E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024112F2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024112F2E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9208.525799999992, "isoelectric_point": 7.784702491760254 }, "id": "DFOKDJOJIG_732", "locus": "GCA013367855_00718" }, { "type": "cds", "sequence": "contig_1", "start": 406017, "stop": 406436, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGAACAAAAATTGTAACTTCATTAAGTCTATGCTTAACTACTGCTATTTCAACAATAGCAATCTATTCAATAATCGCCGTTAAAAAAAATATAGAACTAGATAAGTCAAATAAATATTCATTATTTATACAAGAAAAATCTAGATTGCAATCGCTTGTTGCAAATAATCAACACAAAATTCCAAATAGTTATTTAAATTCAATTCAATATTTCCATAATGTAACTAGAACCTCAAAAGCAATAGAAATAATTGATGCAACATCAAAAATAAGAAGTGAAATTATTGACGTAAATAAAAAAATTCTTGCCTTTGATTTAGAAATAAAAAATCAATTATCGCTATTCAATGAATTTAATCAAATAAAACAAAAGCTTGAAAATGATTATCTTAACAGACTATATAAAATATGA", "aa": "MKKRTKIVTSLSLCLTTAISTIAIYSIIAVKKNIELDKSNKYSLFIQEKSRLQSLVANNQHKIPNSYLNSIQYFHNVTRTSKAIEIIDATSKIRSEIIDVNKKILAFDLEIKNQLSLFNEFNQIKQKLENDYLNRLYKI", "aa_hexdigest": "96413827e707433b5a44da5274091f3f", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16139.61669999999, "isoelectric_point": 9.737772178649902 }, "id": "DFOKDJOJIG_733", "locus": "GCA013367855_00719" }, { "type": "cds", "sequence": "contig_1", "start": 406587, "stop": 407336, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J897", "UniRef:UniRef90_D1J897" ], "nt": "TTGAAAAATATTGAAAATAATATTGATTATTTTTTAGTTAAAAAAGAAGTTGAACATTTACTTGAAAAAATTGAAAGCACTATTTCAATAGTTGAAACTAGACTAGAAAAAAATAAAATCAATAAAATATTTGAGGATAATGAAGTAAAAGAAATTTTAGATTTAATTTCACAATTTAAATTAGATTTACAAAAACAAAAAGATTTATATAACTCAGTAGACAGTAAAACTGATAATATCAAACACGAAACTTTTAAAAAAATATTTAGCCATTATAATAAAAACAATTTCTTTAAAATTTTTGATTGCATAGATCAAAGAGTTGAATTATATTACGACTTTATGTTCTTTTACTCGCCTTTATTCTTTTATGAACAAACAGAAGAAATCAAAAAAGTTTTCCAATATCCTAATAAAAAAGATTTTGAAAAAGAAATAATTTTAAAATATAATCAAGCCAAACAAAATCTTACTAACTTCAAATCAAAGATAAATGGAAATGAAAAAATTGCCAAAAAAGCATTCAATAAATTCAATGAATATATACGCAATTTAGCAATAAGTTTTGAAAAGGAATTAATACAAAAAAAATTATTATTATTAAAAAATTTATTAACTAAATATGATCAAAAGCAAGATGGAAAATCACAATTCATAACAGTTGAAATAAAAAAAGCCATAAATGATGCAGAATCTTTATTAAGTTATAATGACTCGCAATATTCCACAAGAGATAAAAAGAGTATTTGA", "aa": "MKNIENNIDYFLVKKEVEHLLEKIESTISIVETRLEKNKINKIFEDNEVKEILDLISQFKLDLQKQKDLYNSVDSKTDNIKHETFKKIFSHYNKNNFFKIFDCIDQRVELYYDFMFFYSPLFFYEQTEEIKKVFQYPNKKDFEKEIILKYNQAKQNLTNFKSKINGNEKIAKKAFNKFNEYIRNLAISFEKELIQKKLLLLKNLLTKYDQKQDGKSQFITVEIKKAINDAESLLSYNDSQYSTRDKKSI", "aa_hexdigest": "ecfe767c0149e35689f638f29fc4794d", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 406287, "stop": 407387, "observations": { "mutation": [ 406434, 406536, 407334 ], "stop": [ 406434, 406536, 407334 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J897", "query_cov": 1.0, "subject_cov": 0.5460526315789473, "identity": 0.996, "score": 465.0, "evalue": 5.62e-162, "gene_start": 1, "gene_end": 249, "reference_start": 191, "reference_end": 439, "reference_sequence": "MKKRTKIVTSLSLCLTTAISTIAIYSIIAVKKNIELDKSNKYSLFIQEKSRLQSLVANNQHKIPNSYLNSIQYFHNVTRTSKAIEIIDATSKIRSEIIDVNKKILAFDLEIKNQLSLFNEFNQIKQKLENDYLNRLYKIWRADAGEPNMIVDFGFFEEETHFQKYLETIKKYSWISKNSSSFEIKRAIFDLKNIENNIDYFLVKKEVEHLLEKIESTISIVETRLEKNKINKIFEDNEVKEILDLISQFKLDLQKQKDLYNSVDSKTDNIKHETFKKIFSHYNKNNFFKIFDCIDQRVELYYDFMFFYSPLFFYEQTEEIKKVFQYPNKKDFEKEIILKYNQAKQNLTNFKSKINGNEKIAKKAFNKFNEYIRNLAISFEKELIQKKLLLLKNLLTKYDQKQDGKSQFITVEIKKAINDAESLLSYNDSQYSTRDKKSIWEFRVTKNNLQELIDKY" }, "paralog": false, "identity": 0.9918032786885246, "score": 711.0, "evalue": 2.01e-261, "description": "Internal stop codon at 406434, 406536, 407334. Nonsense mutation around 406434, 406536, 407334.", "psc": { "uniref90_id": "UniRef90_D1J897", "uniref50_id": "UniRef50_D1J897", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J897", "UniRef:UniRef50_D1J897" ] }, "pscc": { "uniref50_id": "UniRef50_D1J897", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J897" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 30016.070400000048, "isoelectric_point": 8.962088584899902 }, "id": "DFOKDJOJIG_734", "locus": "GCA013367855_00720" }, { "type": "cds", "sequence": "contig_1", "start": 407512, "stop": 407745, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA961", "UniRef:UniRef90_UPI00215DA961" ], "nt": "TTGAAATTGATATCATCAAATGTTGAGGAATTTAATATTCTTGCAAATGATGTCTATTCTAAAAATCAAAAAATTTCTAACGAAATACAAGAAGTTAGAAAACTTGTAGAAGAACACGAAGAAAAATTAGAAGATTTTTATATTAAGAAATCACAGCCAAATTTAAAATATTTAACCGAAATATTACTTAAATTACAAAATAAATTACATTCAATTAGACAAAAAGTCAATTAA", "aa": "MKLISSNVEEFNILANDVYSKNQKISNEIQEVRKLVEEHEEKLEDFYIKKSQPNLKYLTEILLKLQNKLHSIRQKVN", "aa_hexdigest": "501eb2788bab4424ef041eea649ebd96", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 407512, "stop": 407952, "observations": { "mutation": [ 407754 ], "stop": [ 407754 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215DA961", "query_cov": 1.0, "subject_cov": 0.5167785234899329, "identity": 0.8959999999999999, "score": 130.0, "evalue": 3.77e-37, "gene_start": 1, "gene_end": 77, "reference_start": 71, "reference_end": 147, "reference_sequence": "MLNESLKEQKNSEDGNFLSKFLNNEIAKILKENSKLFSSSDIAKINKNIEITYELSRKLNLEIILNRWNLLKLISSNVEEFNILANDVYSKNQKISNEIQEVKKLIEEHDKKLEDFYIKKLQPNLKYLTEILLKLQNKLYLIRQKVNQI" }, "paralog": false, "identity": 0.9315068493150684, "score": 262.0, "evalue": 7.86e-92, "description": "Internal stop codon at 407754. Nonsense mutation around 407754.", "psc": { "uniref90_id": "UniRef90_UPI00215DA961", "uniref50_id": "UniRef50_UPI00215DA961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215DA961", "UniRef:UniRef50_UPI00215DA961" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA961" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9215.500399999994, "isoelectric_point": 7.953093910217285 }, "id": "DFOKDJOJIG_735", "locus": "GCA013367855_00721" }, { "type": "cds", "sequence": "contig_1", "start": 407752, "stop": 408342, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410C12B", "UniRef:UniRef90_UPI002410C12B" ], "nt": "ATGTTGAACGAATTATTTAATGATTTCAAGGATATTGATTCAAAAACATTTGCAGAAGTTATTTATCAATTTGAAGAATCAAATAAATTATTGGCCGAATATATTGAATTATATTCACAAATTATTTATAATGCAAAGAATGAATTTGAAATGCAACAAAAAAATAGCGATTTATTTAATTTATTTAGTCAAGAAACAAAAAATAAAATTACAAATTTTTTAGCAACAATTAATAAATTGGAAAATAATAGAAATGATCAACAAATAAAAAAATCTATACATGAAGAATTTAAAAAAATTCTAACTGTCCAAGAAGAAATTTATAAAAATATAAATAAGGATTTTTTAAACATTACTAATAAAGAAGAAAGCATTAAACTTTTAAAAACGCTTAATGAAAGTTTAAAAGAACAAAAAAATTCAGAAGATGGAAATTTTTTAAGTAAATTTCTAAATAATGAAATAGCCAAAATATTAAAAGAAAATTCTGAGCTATTTTCTTCAAAAGATATTGCCAAAATCAATAAAAATATAGAAATTACATACGAACTTTCTAGAAAATTAAACTTAGAAATTATTTTAAATAGGTGA", "aa": "MLNELFNDFKDIDSKTFAEVIYQFEESNKLLAEYIELYSQIIYNAKNEFEMQQKNSDLFNLFSQETKNKITNFLATINKLENNRNDQQIKKSIHEEFKKILTVQEEIYKNINKDFLNITNKEESIKLLKTLNESLKEQKNSEDGNFLSKFLNNEIAKILKENSELFSSKDIAKINKNIEITYELSRKLNLEIILNR", "aa_hexdigest": "df7a3d6641f25ea6e3757ffabb0ec03c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 407512, "stop": 408642, "observations": { "mutation": [ 407754, 408363, 408606 ], "stop": [ 407754, 408363, 408606 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410C12B", "query_cov": 1.0, "subject_cov": 0.47115384615384615, "identity": 0.9690000000000001, "score": 346.0, "evalue": 1.43e-116, "gene_start": 1, "gene_end": 196, "reference_start": 141, "reference_end": 336, "reference_sequence": "MKSKEILWNISVQKNLIDQRYTKESLSPKSKIDVEFLKQKRKLENLFSIYKKWNKADMSKEIELLKKINNVNFGFKNDDIAKLTSEISKRIEDIYQKINSSKDIQKHLNNVKNDLNPLIQELINFNNFKNSKTWDVVDRLMLNELFNDFKDIDSKTFAEVIYQFEESNKLLAEYIELYSQIIYNAKNEFEMQQKNSDLFNLFSQETKNKITDFLAIINKLENNRNDQQIKKSIHEEFKKILTVQEEIYKNINKDFLNITNKEESIRLLKMLNKNLKEQKNSEDGNFLSKFLNNEIAKILKENSELFSSKDIAKINKNIEITYELSRKLNLEIILNRWNLLKLISSNVEEFNILANDVYSKNQKISNEIQEVRKLIEEHEEKLEDFYIKKSQLILKYLTEILLKLQNKLHSIRQKVN" }, "paralog": false, "identity": 0.9601063829787234, "score": 693.0, "evalue": 5.67e-256, "description": "Internal stop codon at 407754, 408363, 408606. Nonsense mutation around 407754, 408363, 408606.", "psc": { "uniref90_id": "UniRef90_UPI002410C12B", "uniref50_id": "UniRef50_UPI002410C12B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410C12B", "UniRef:UniRef50_UPI002410C12B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410C12B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410C12B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23295.150400000035, "isoelectric_point": 5.347207832336425 }, "id": "DFOKDJOJIG_736", "locus": "GCA013367855_00722" }, { "type": "cds", "sequence": "contig_1", "start": 408361, "stop": 408591, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAGGAAATAGAACTTCTTAAAAAGATTAATAATGTTAATTTTAGCTTTAAAAACGAAGATATAGCGAAATTAACTAGTGAAATTTCAAAAAGAATTGAAGATATTTATCAAAAAATAAATTCATCAAAAGATATACAAAAACATTTAAATAATGTTAAGAATGATTTAAATCCACTTATACAAGAATTGATTAACTTTAATAATTTTAAAAATAGCAAAACATGA", "aa": "MSKEIELLKKINNVNFSFKNEDIAKLTSEISKRIEDIYQKINSSKDIQKHLNNVKNDLNPLIQELINFNNFKNSKT", "aa_hexdigest": "43482a39934705cbb10a0338adb48bdd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8937.110499999995, "isoelectric_point": 9.26070613861084 }, "id": "DFOKDJOJIG_737", "locus": "GCA013367855_00723" }, { "type": "cds", "sequence": "contig_1", "start": 408739, "stop": 409194, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACGAAAAAACAAACTATTTATTCTATCAACTATCTTAGGATCGTTTGCAACTGTATTTACAGTAATTTCTAGTGCTTCATGTTCTAATAAACAAGATAAAAAAGAAGAAACTAAAGATAGAAAAGAAGATTCAAACAACAACGTAGCAAATACGCCTAATGTTAAACCAAATACATCTAATAAAAAACAAGAAGATAATAGCCACATAGTTAGTCCTAATATAAAGCCAACTGTTCCAAATGAATCTCAAAAACCAAAAGAAGATAAAAATAATGTAGCAAGTTTTAGTCCTAGTAGGCTACAAGAAGTTAAAAATGAACTGCAAACATATATAGCCAATAATCTTAAAGATTTTATTTATGAAAATTTAAAATTAGAAATTAAAGATTTTATTGAAAAAGATAAAAATGAGGCTGATGAATTTATTTTGAAATCTAAAGAAATTCTTTGA", "aa": "MKRKNKLFILSTILGSFATVFTVISSASCSNKQDKKEETKDRKEDSNNNVANTPNVKPNTSNKKQEDNSHIVSPNIKPTVPNESQKPKEDKNNVASFSPSRLQEVKNELQTYIANNLKDFIYENLKLEIKDFIEKDKNEADEFILKSKEIL", "aa_hexdigest": "e26a3dec89edadefe7669e61da6090c9", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17262.227899999998, "isoelectric_point": 8.836955451965334 }, "id": "DFOKDJOJIG_738", "locus": "GCA013367855_00724" }, { "type": "tRNA", "sequence": "contig_1", "start": 409404, "stop": 409487, "strand": "+", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 70.9, "nt": "GGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 409410, 409412 ], "id": "DFOKDJOJIG_20", "locus": "GCA013367855_00725" }, { "type": "tRNA", "sequence": "contig_1", "start": 409491, "stop": 409565, "strand": "+", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 65.1, "nt": "TGCCCTTTAGCCAAGTGGAAAGGCAGCGGTTTTTGGTACCGCCATCCGTTAGTTCGAATCTAACAAGGGCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 409523, 409525 ], "id": "DFOKDJOJIG_21", "locus": "GCA013367855_00726" }, { "type": "cds", "sequence": "contig_1", "start": 409670, "stop": 410032, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00093A2145", "UniRef:UniRef50_UPI001C669CA5", "UniRef:UniRef90_UPI001C669CA5" ], "nt": "ATGGATGCTCTTGAGTTGCAAGGACAAGAAATACAGGAAGATAAATTAGAAAAATTTAAATCTGATTTTAGAGCATTAAAAAAACCAATTGAAGATAAGATGAATGAAATTATTGGTGAATTTTTTGATTTCATAAATAATTTGTATGGCACAAATAGTAATACATCTTCTTTTATTGAAAAAAATCTTAAAGGAAAAGTTGAATATAAAGAAGGCTACGAAGCTTTAAACAAATTAATACAAGAAGCAGAAAAAATTTTGGACAACCCAGGAGAACATAGCAATTTAGAACTAAAAAATAAAGATATTAAAATACAAGCCAAATTGCTTGAATATAAAAATAAATATAATATGAACAAATAA", "aa": "MDALELQGQEIQEDKLEKFKSDFRALKKPIEDKMNEIIGEFFDFINNLYGTNSNTSSFIEKNLKGKVEYKEGYEALNKLIQEAEKILDNPGEHSNLELKNKDIKIQAKLLEYKNKYNMNK", "aa_hexdigest": "b3d4ad06610ea36cc91774b8983c0a2c", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00093A2145", "query_cov": 0.9916666666666667, "subject_cov": 0.815068493150685, "identity": 0.7390000000000001, "score": 160.0, "evalue": 1.33e-48, "valid": false, "uniref50_id": "UniRef50_UPI00093A2145" }, "pscc": { "uniref50_id": "UniRef50_UPI00093A2145", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00093A2145" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 409670, "stop": 410299, "observations": { "mutation": [ 410068, 410206 ], "stop": [ 410068, 410206 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001C669CA5", "query_cov": 1.0, "subject_cov": 0.5741626794258373, "identity": 0.925, "score": 209.0, "evalue": 4.74e-67, "gene_start": 1, "gene_end": 120, "reference_start": 90, "reference_end": 209, "reference_sequence": "KKIIDHLNAQLNLFKDITSASDIQQISVAKQWLTKDLNNAKEDKFSCDISCFVNLEVPEFIGDMQSKSLTCLDSKKYWEEPKKEIERIRMDALDLERQGIQEDKLEKFKSDFRALKKPIEDKMNEIIGEFFDFINNLYGTNSNTSSFIENKLKGKVEYKEGYEALNKLIQEAEKILDNPGEHSNLELKNKEAEIQAKLLEYKNKYNMNK" }, "paralog": false, "identity": 0.9473684210526315, "score": 391.0, "evalue": 5.05e-141, "description": "Internal stop codon at 410068, 410206. Nonsense mutation around 410068, 410206.", "psc": { "uniref90_id": "UniRef90_UPI001C669CA5", "uniref50_id": "UniRef50_UPI001C669CA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001C669CA5", "UniRef:UniRef50_UPI001C669CA5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001C669CA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001C669CA5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14065.80419999998, "isoelectric_point": 5.334930610656738 }, "id": "DFOKDJOJIG_739", "locus": "GCA013367855_00727" }, { "type": "cds", "sequence": "contig_1", "start": 410204, "stop": 412060, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9Q4", "UniRef:UniRef90_UPI00158F4959" ], "nt": "ATGAAAAAATCGAAATTAAGAATTTTACTTTCTACTTCGTTAATTATTGCTCTAGCAACAACGGCAACCTTAGTTGCAACATCACTAAAAAGTAAATTTAAAAGAAGACAATATGAACAAGAAGATAATATTGACAAATTAAAGGAAAAATTTAATAGATCAAAATCTAAATTAGATAATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAATACAAATCTTGCTGGTAAAGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTAACTAAGAAAATTAATGATAAAAAAGATAATCTTCTTAAAGATTTTAACGACGCTAAAAAGAAATTGCAAGATTTAATTAATTCACAAGACGGCCAAAAAGTCGACACTTCTAAAGCAAATCAATCATTGCAAAACAATAATGTAGATACTTCATCAACAACCGATCAAATTGTTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAAAAAGCTAAACAAGAATTTAATTCAAAGAAGCAGCAATTAGATAATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAAGAAGAAACTAACACCTTTAACAATACAAATCTTACTGGCCAAGATTTAATTAAAGACATTGAGTCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTGACCAAGAAAATTAATGATAAAAAGAACCAAAAAGACAATCTTCTTAAAGATTTTAATGACGCTAAAAAGCAATTAGAAGACTTAATTAATTCACAAGATGGTCAAAAAGTTGACACTTCTAAAGCAAATCAATCATTGCAAAACAAGAATGTAGATGCTTCATCAACGACTGATCAAATTATTAACGCAACAAATGAAATTAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAACAAGCCACACAAGACTTTAATTCAAAGAAGCAACAATTAGATGATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAATCAGAAACCAACATCTTTGACAACACAAATCTTACTAACAAAGATTTAATTAAAGACATTGAGTCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTAACTAAGAAAGTTAATGATAAAAAGAACCAAAAAGACAATCTTCTTAAAGACTTTAATGACGCTAAAAAGCAATTAGAAGACTTAATCAATTCACAAGACGCACAAAAAGTCGACAAATCTAATGCAAATCAATCATTGAAAAATAACAATGCAGATATTTCATCGACGACCGATCAAATTGTTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAAAATGCTAAACAAAACTTTGATTCAAAGAAACAACAACTAGATAATTTGATTAAATCAAATGAAGCCAAAGATGTTGATAAACAATCAGAAACCAACATCTTTGACAACACAAATCTTACTAACAAAGATTTAATTAAAGACATTGAGTCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTAACTAAGAAAATTAATGATAAAAAACCAAAGGAAAATATTGAATATGAAGGGCTTGAAAAAATCAGGGAACAAATTAAACAATTTATTGAAAAAGTAAAAGAGGATGAATATTATAAAGAATATAATAGTAACTATTATGATTATAAAAAAATAATAGACCATTTAAACGCACAACTTAATCTCTTTAAAGATATTACTTCTGCTTCTGACATTCAACAAATATCCGTGGCTAAGCAATGA", "aa": "MKKSKLRILLSTSLIIALATTATLVATSLKSKFKRRQYEQEDNIDKLKEKFNRSKSKLDNLIKSNEAKDVDKQPETDIFNNTNLAGKDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKKLQDLINSQDGQKVDTSKANQSLQNNNVDTSSTTDQIVNATNEIKKATQDLQKLIDAAKEKAKQEFNSKKQQLDNLIKSNEAKDVDKQEETNTFNNTNLTGQDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNKNVDASSTTDQIINATNEIKKATQDLQKLIDAAKEQATQDFNSKKQQLDDLIKSNEAKDVDKQSETNIFDNTNLTNKDLIKDIESKTKTIEDAIKSLTKKVNDKKNQKDNLLKDFNDAKKQLEDLINSQDAQKVDKSNANQSLKNNNADISSTTDQIVNATNEIKKATQDLQKLIDAAKENAKQNFDSKKQQLDNLIKSNEAKDVDKQSETNIFDNTNLTNKDLIKDIESKTKTIEDAIKSLTKKINDKKPKENIEYEGLEKIREQIKQFIEKVKEDEYYKEYNSNYYDYKKIIDHLNAQLNLFKDITSASDIQQISVAKQ", "aa_hexdigest": "6d0818f512b40626c84b522345473752", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 409901, "stop": 412060, "observations": { "mutation": [ 410068, 410206 ], "stop": [ 410068, 410206 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158F4959", "query_cov": 1.0, "subject_cov": 0.7763819095477387, "identity": 1.0, "score": 855.0, "evalue": 1.5e-304, "gene_start": 1, "gene_end": 618, "reference_start": 1, "reference_end": 618, "reference_sequence": "MKKSKLRILLSTSLIIALATTATLVATSLKSKFKRRQYEQEDNIDKLKEKFNRSKSKLDNLIKSNEAKDVDKQPETDIFNNTNLAGKDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKKLQDLINSQDGQKVDTSKANQSLQNNNVDTSSTTDQIVNATNEIKKATQDLQKLIDAAKEKAKQEFNSKKQQLDNLIKSNEAKDVDKQEETNTFNNTNLTGQDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNKNVDASSTTDQIINATNEIKKATQDLQKLIDAAKEQATQDFNSKKQQLDDLIKSNEAKDVDKQSETNIFDNTNLTNKDLIKDIESKTKTIEDAIKSLTKKVNDKKNQKDNLLKDFNDAKKQLEDLINSQDAQKVDKSNANQSLKNNNADISSTTDQIVNATNEIKKATQDLQKLIDAAKENAKQNFDSKKQQLDNLIKSNEAKDVDKQSETNIFDNTNLTNKDLIKDIESKTKTIEDAIKSLTKKINDKKPKENIEYEGLEKIREQIKQFIEKVKEDEYYKEYNSNYYDYKKIIDHLNAQLNLFKDITSASDIQQISVAKQWLTKDLNNAKEDKFSCDISCFVNLEVPEFIGDMQSKSLTCLDSKKYWEEPKKEIERIRMDALELQGQEIQEDKLEKFKSDFRALKKPIEDKMNEIIGEFFDFINNLYGTNSNTSSFIEKNLKGKVEYKEGYEALNKLIQEAEKILDNPGEHSNLELKNKDIKIQAKLLEYKNKYNMNK" }, "paralog": false, "identity": 0.9972183588317107, "score": 1128.0, "evalue": 0.0, "description": "Internal stop codon at 410068, 410206. Nonsense mutation around 410068, 410206.", "psc": { "uniref90_id": "UniRef90_UPI00158F4959", "uniref50_id": "UniRef50_A0A454C9Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158F4959", "UniRef:UniRef50_A0A454C9Q4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9Q4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 70363.64940000031, "isoelectric_point": 7.477760505676271 }, "id": "DFOKDJOJIG_740", "locus": "GCA013367855_00728" }, { "type": "cds", "sequence": "contig_1", "start": 412319, "stop": 413596, "strand": "-", "frame": 3, "gene": "atpD", "product": "MSC_0618 family F1-like ATPase beta subunit", "db_xrefs": [ "COG:C", "COG:COG0055", "SO:0001217", "UniRef:UniRef50_A0A449A4P1", "UniRef:UniRef90_D1J8A0" ], "nt": "TTGACATTGCATGAAGGAAAAACCTTTTTACTAGTAAAAAGATTAATTGACGAAAAACACGTTAGAGCAATTGTTGTATATGCAAGTAAAGATATTTCAATTAACGAAGAAGTTGTTAACACTCATAAGAGTTTTTTGGTTCCAATAGGGCAAAATTCAAAAAACAATATTTACAACTTTTGGGGTCAACCTTTACTAGAAACAAAGAATAAAATTGAATATGTAGAAATGAATTCAACTATAAACAATGAAAGATATCTAGAAAATAACAAAGAAGTTATAGAAACCGGAATAAAAGCAATTGATTTCTTTATGCCAATTGTTAAGGGCGCAAAAATTGGTTTATTTGGAGGAGCCGGAGTTGGAAAAACTGTTCTAATGAAGGAAATTATTTTTAACCTTAATAAACAACATGAAGCAAATTCTAATATCTTTATTGGTTCGGGTGAACGTTCAAGAGAAGGAATAGAATTATACAATGAACTTTCTTCATCTAATTTAATGAAGGACTCAATAATGTTTATTTCAAAAATGAATGAAGCACCAGGAGCTAGAACGTCAATTGTTCCAATTGGAATTACTGCTGCAGAATATTTAAGAGACACATGCAATGAAAATGTTTTATTATTTATTGACAATATTTATCGTTTTATTCAAGCAGAAAATGAAGTAAGTTCAGCATTAGGCAAAAAGCCTTCAATTGGAGGTTATCAATCAACTTTAGAAAGTGATGTAACTGAAGTTGAAAGCAGATTGTTTAGAAACAAGAAGGGCTCAATAACTTCATTCCAAACAATATTTCTACCAATGGATGATTTAAGCGATCCTTCTGCAGTAGCCGTATTTAATCACTTAGATGGTAATTTGGTCTTATCTAGATCTCAAGCGGCTAAAAATATTTTTCCTGCCTTTGATCCACTTGCTTCATCTACAAACGCAATTAATCCAACCATTATTGGCAAACGCCACTATGACGCAATAATTGAAGCAAAAAGAATATTAAAGGCATATAAAGATCTTGAAGATGTTATTCTGATATTGGGATTTGATGAACTTGATGAAGAAAGCAAAATCACTGTTAAAAAAGCTCTTCAACTTGAAATGTTTTTTACTCAAAATTTCTTTATGACTGAACATTTTACTAAATCGCCTGGTCAATTTGTGCCATTAAACGAAACAGTTGAAAGCGTTATAAGAATATTGGAAGGAAGATATTTAAAACAAAATCCTGAAATTTTTGCTTATATAGGTTCAAACAAAGATATTCCACAAGATTAA", "aa": "MTLHEGKTFLLVKRLIDEKHVRAIVVYASKDISINEEVVNTHKSFLVPIGQNSKNNIYNFWGQPLLETKNKIEYVEMNSTINNERYLENNKEVIETGIKAIDFFMPIVKGAKIGLFGGAGVGKTVLMKEIIFNLNKQHEANSNIFIGSGERSREGIELYNELSSSNLMKDSIMFISKMNEAPGARTSIVPIGITAAEYLRDTCNENVLLFIDNIYRFIQAENEVSSALGKKPSIGGYQSTLESDVTEVESRLFRNKKGSITSFQTIFLPMDDLSDPSAVAVFNHLDGNLVLSRSQAAKNIFPAFDPLASSTNAINPTIIGKRHYDAIIEAKRILKAYKDLEDVILILGFDELDEESKITVKKALQLEMFFTQNFFMTEHFTKSPGQFVPLNETVESVIRILEGRYLKQNPEIFAYIGSNKDIPQD", "aa_hexdigest": "a219c4dbef6636e08aa3f76c1d3d8b39", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8A0", "query_cov": 1.0, "subject_cov": 0.9361233480176211, "identity": 0.998, "score": 825.0, "evalue": 2.04e-301, "valid": true, "gene": "AtpD", "product": "MSC_0618 family F1-like ATPase beta subunit", "uniref50_id": "UniRef50_A0A449A4P1", "cog_id": "COG0055", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_A0A449A4P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A4P1" ], "product": "F0F1 ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "DFOKDJOJIG_741", "locus": "GCA013367855_00729" }, { "type": "cds", "sequence": "contig_1", "start": 413758, "stop": 413940, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGGAATAAAAGATGAACAAAAAGCATTTATGTTACTAGATTATTTAATAAATAATTATAACGATTGCATAAAAGCATTTGAAACAATTTTAAATAATTTAGACTACGATGATAATATTATGAAGAATTATTTTGCATTTGTCTTGAAATCATATTCGGACTATTTAAATTTAAATTGA", "aa": "MQGIKDEQKAFMLLDYLINNYNDCIKAFETILNNLDYDDNIMKNYFAFVLKSYSDYLNLN", "aa_hexdigest": "89190d50c19cbaf243e99e5850e4f55d", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7186.087000000001, "isoelectric_point": 4.268915367126465 }, "id": "DFOKDJOJIG_742", "locus": "GCA013367855_00730" }, { "type": "cds", "sequence": "contig_1", "start": 413947, "stop": 414891, "strand": "-", "frame": 1, "gene": null, "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FAQ0" ], "nt": "ATGTTAAGCAATCATCATCCAATATTCGGTCTAGCTCATAATTTGATGACAGTTAAAACATTAAATGAACAACTTTATACTGGAATTATTGCAATAGATTTATTAATTCCAATTGGAAAAGGTCAACGTGAATTAATTATTGGTGATAGACAAACAGGCAAAACTCATATGGCATTAAACGCAATTATAAATCAAGCAAAAAATGGCACAAAATGTATTTATGTTGCAATTGGCCAAAAGCGTGAATCATTAACTAATATTTATGAAACATTAAAAAAATACGATGCATTGAAAAACACTATGATTATTGATGCGCCTCAAAACGTTTATGAACAATATCTAGCGCCTTACATTGGAATGGCTCATGCAGAAAATCTTTCATATGACAACGATGTTTTAATAGTATTTGATGATTTAACTAAACATGCTAATATTATTAGAGAAATTGCCCTATTAACCAACAAGTCAGTTGGAAAAGAAGCAATGCCTAGTGACATCTTTTTTGCCCACTCATCATTGCTTGAAAGGGCTGGATCTTTTGTTAATAGAAAAACAATTACTGCACTTCCAATTTTGCAAACAGTTGATGGTGATATAACTAGTTTAATTTCATCTAATATAATTTCAATAACTGATGGTCAAATTGTAACTTCTTCTGACTTATTTGCTTCTGGAAAATTACCTGCTATAAACATTGATTTGTCTGTTTCTAGAACAGGTTCAAGTGTTCAATCAAGAATGATGACCAAAATAGCGGGTGAAATTAATAAAATTTTTAGAAGTTACAAGAGACATTTAAAATTGGCAATGTTAGATTACGAGCTTAATAAAGAAACTTCTATTTTGCTTCACAAAGGAAAATTAATTGACAAAATGTTCTTGCAAAAGGGTTTTTCACTTTACTCATATCGTTTTGTAGTGCTAACAACAAAATTAATTTCTTGA", "aa": "MLSNHHPIFGLAHNLMTVKTLNEQLYTGIIAIDLLIPIGKGQRELIIGDRQTGKTHMALNAIINQAKNGTKCIYVAIGQKRESLTNIYETLKKYDALKNTMIIDAPQNVYEQYLAPYIGMAHAENLSYDNDVLIVFDDLTKHANIIREIALLTNKSVGKEAMPSDIFFAHSSLLERAGSFVNRKTITALPILQTVDGDITSLISSNIISITDGQIVTSSDLFASGKLPAINIDLSVSRTGSSVQSRMMTKIAGEINKIFRSYKRHLKLAMLDYELNKETSILLHKGKLIDKMFLQKGFSLYSYRFVVLTTKLIS", "aa_hexdigest": "191f1297b78296469ae700fd94d6d26a", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A432FAQ0", "query_cov": 0.8853503184713376, "subject_cov": 0.8938906752411575, "identity": 0.525, "score": 251.0, "evalue": 1.05e-78, "valid": false, "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein", "uniref50_id": "UniRef50_A0A432FAQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A432FAQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FAQ0" ], "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_743", "locus": "GCA013367855_00731" }, { "type": "cds", "sequence": "contig_1", "start": 414925, "stop": 415215, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAACACAACTAAAAATCCTCGTATTAAATCAATATTCAATTGCATAGCAACAATTGAGGGAGAATATAACTACCGCCAAAATCAACAATTTTATTTAGCAAGTAACCCAAATGTTAAATTCATAGTTATTTCTGCTACAACTAATAAGGCATTCATGTTGATTAATAGCGAACACGTATCTTTAGATATAAATAGTGAATTAATTGAATTAAATGAATCCCAAAATATAAATACTAGTCTTGATTATTTGGGCAAAATTATTGATATTAACGGTGGCATTATTTGA", "aa": "MNNTTKNPRIKSIFNCIATIEGEYNYRQNQQFYLASNPNVKFIVISATTNKAFMLINSEHVSLDINSELIELNESQNINTSLDYLGKIIDINGGII", "aa_hexdigest": "847cb441a179908963e96e9bf3c768b2", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10856.16159999999, "isoelectric_point": 5.187547111511229 }, "id": "DFOKDJOJIG_744", "locus": "GCA013367855_00732" }, { "type": "cds", "sequence": "contig_1", "start": 415231, "stop": 415560, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTAAGATAAAACATAATTTTGACTTATTATCAAATTTAGCAAGTAATATTTCAATAAAAGCTAATAAAGAAGAAAAAGAAAATTTCAATAAAGCTTATTTAGAAGCAGTCAATGTTTTAAATAAAAATTATCTTGAACAAAAGAAGATTATTAAAAAACTAGGGACATCTTTTATAGTTATTTCTTTAATCATAATAATTACAAACATAATAATTTTTGCATTGTTAAAAAACTTAAAATTAAAGAAAAAGGTAATTATTATCAATAGTGTAATACTAGCGATCGCATTACTGATATTAATAATTGGAGGGCTATTATTTATATAG", "aa": "MSKIKHNFDLLSNLASNISIKANKEEKENFNKAYLEAVNVLNKNYLEQKKIIKKLGTSFIVISLIIIITNIIIFALLKNLKLKKKVIIINSVILAIALLILIIGGLLFI", "aa_hexdigest": "a56e2badc1fdc372f5e5b5a500cf93c3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12272.90529999998, "isoelectric_point": 10.000094032287599 }, "id": "DFOKDJOJIG_745", "locus": "GCA013367855_00733" }, { "type": "cds", "sequence": "contig_1", "start": 415582, "stop": 415749, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTTAGTGAAGCACTTAAATTCAATGAAAAATTCATTAAAACAAATTTAGCAGAAATCAAAGGCAATGAGAATGTTTTAAGAGAATTATTCTTATTAAATAAACAATTAAACTACAAATTTACCGCTTTAATTAGCCAAAGAGATTTCAACCAAGAAAAATGA", "aa": "MQFSEALKFNEKFIKTNLAEIKGNENVLRELFLLNKQLNYKFTALISQRDFNQEK", "aa_hexdigest": "5bc6f80562418da7b41a1dde956cf919", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6596.5232, "isoelectric_point": 9.156976509094239 }, "id": "DFOKDJOJIG_746", "locus": "GCA013367855_00734" }, { "type": "cds", "sequence": "contig_1", "start": 415783, "stop": 415896, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAAGATAAAACAAATACAAAAAAATTACTTGAAGAGTTTGGCAAAACAATTCGTTATACATTTTTTTCTTATTTAAATAGTCTAGTAGTAAATAATATATACTTTTGA", "aa": "MQEDKTNTKKLLEEFGKTIRYTFFSYLNSLVVNNIYF", "aa_hexdigest": "032ed36e2b0edcece9506d51b6c0f10f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4496.099100000001, "isoelectric_point": 8.063979530334471 }, "id": "DFOKDJOJIG_747", "locus": "GCA013367855_00735" }, { "type": "cds", "sequence": "contig_1", "start": 415903, "stop": 416478, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGATTTAAAACCATTAGTTGATGGTGATAAAACAAGTCACTTAACAAAAGAACAAGAAAAAAATTTAATTCAATCATTGCCCCTTTTGGTTCCGTTTATATCAAGTAATAATTTGCCTTCATCATTAAGTCAAATTAAAAGTCAATTTGATTCATTAGATGCAAGCAAACGTCAAGAATTATTTTATTTCAATAACCCAATAAATACTCGTTATAAATATTCTGTTTTTTCATTTGATATTAGTGGCAATGATATTATTAATTTAAAAGTACATATTTCAGATAATATAAATCCTAAGTTAGAAAGAACATATTTAATTGAAAAATATACTCCGTCACAAGACAAATATTTTAATCTCTTAAAAGAAACTGAAATAAAATCAATTTCAGAAATATTTAAGCCATTATACAAAGGTTTGGGCTTAGATGAAAAGTTAGATTACAATAAGCTAAGAAATGGATATTTAAGAAAAGCATTGCTTGCAATGATTGACTCAGCGCTACAAATGTCATTTAAAGCTGACAAAGATTCATATAGTGCAATTAGCAATAAAATATTGCAAAAATATTGA", "aa": "MPDLKPLVDGDKTSHLTKEQEKNLIQSLPLLVPFISSNNLPSSLSQIKSQFDSLDASKRQELFYFNNPINTRYKYSVFSFDISGNDIINLKVHISDNINPKLERTYLIEKYTPSQDKYFNLLKETEIKSISEIFKPLYKGLGLDEKLDYNKLRNGYLRKALLAMIDSALQMSFKADKDSYSAISNKILQKY", "aa_hexdigest": "9734bfae9b82f96701ed6e49bf8155f9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22059.017900000003, "isoelectric_point": 9.001156425476076 }, "id": "DFOKDJOJIG_748", "locus": "GCA013367855_00736" }, { "type": "cds", "sequence": "contig_1", "start": 416587, "stop": 417285, "strand": "-", "frame": 1, "gene": null, "product": "MSC_0620 family F1-like ATPase-associated subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D97E37", "UniRef:UniRef90_UPI0034D97E37" ], "nt": "ATGAATAAAAAGAAAATCTTATTATTATCATTTGCAATAGTTCCTTTATTATCTCCAATAGTAGTTTCGGCAGCGTCAAATAATGATCCAAATAATAAAAAACCTGAGTTAGATCCAAATTTTGGCGAATTTGAAAGCGAAGCTAAAAAAACAATTGCTTCAACATTGGATGATGGTTTAAAAGCAATAATCAATTATTTGCAAGTTCAACAACAAAGATTGCTAGAAAATAAAGAATTAGAATTTAAATTAAAAATTCAACAATTAATTTATTTAAAAAATTTAGAAACATTTCTAACAAAAAATAAAGAAGAAATTAAAAAAGATCCTAATAAGTATGGAATTTATTTAAATACTCCCCTTATATTAGGCAAAGAAAAAAACCACGATTTGCAAGATATTGATTATGAAGGCGATTTATTTAAATCAATAAAAACTGGTAAAACCGATCCACTAGATTATAAAAAAGCAATTTTGCCAAAAGGAAGTGTTAAAGAAGTTGCTAAAGATCAATTAAATACTGTTAAAAAAGATAAGTATTATCAATTTTTAAAGAAATATAAATCTGATTTTTTAAAAGAAGTCAATAAGTTATTTTTTAATGAAAAAGACGTTTTAAATATTGACAAAGATGTCGAATTAATTAGGGATAAAAACGGCGTATTTTCTACCACTTTGCCCAAAGGCTTTAAAAGTTGA", "aa": "MNKKKILLLSFAIVPLLSPIVVSAASNNDPNNKKPELDPNFGEFESEAKKTIASTLDDGLKAIINYLQVQQQRLLENKELEFKLKIQQLIYLKNLETFLTKNKEEIKKDPNKYGIYLNTPLILGKEKNHDLQDIDYEGDLFKSIKTGKTDPLDYKKAILPKGSVKEVAKDQLNTVKKDKYYQFLKKYKSDFLKEVNKLFFNEKDVLNIDKDVELIRDKNGVFSTTLPKGFKS", "aa_hexdigest": "f290a6267009496fabeff435a730a3f8", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 416284, "stop": 417285, "observations": { "mutation": [ 416589 ], "stop": [ 416589 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D97E37", "query_cov": 1.0, "subject_cov": 0.6609686609686609, "identity": 0.9740000000000001, "score": 432.0, "evalue": 4.45e-151, "gene_start": 1, "gene_end": 232, "reference_start": 1, "reference_end": 232, "reference_sequence": "MNKKKILLLSFATVPLLSPIVVSAASNNDPNNKKPELDPNFGEFESEAKKTISSTIEDGLKAIINYLQVQQQRLLENKELEFKLKIQQLIYLKNLETFLTKNKEEIQKDPNKYGIYLNTPLILGKEKNHDLQDIDYEGDLFKSIKTGKTDPLDYKKAILPKGSVKEVAKDQLNAVKKDKYYQFLKKYKSDFLKEVNKLFFNEKDVLNIDKDVELIRDKNGVFSTTLPKGFKSWDEYFISKFKVRITKFDLKQNQETNEAEQKEEKNPDSLPDLKPLVDGDKTSHLTKEQEKNLIQSLPLLVPFISSNNLPSSLSQIKSQFDSLDASKRQELFYFNNPINTRYKYSVFFIWY" }, "paralog": false, "identity": 0.978978978978979, "score": 643.0, "evalue": 1.31e-236, "description": "Internal stop codon at 416589. Nonsense mutation around 416589.", "psc": { "uniref90_id": "UniRef90_UPI0034D97E37", "product": "MSC_0620 family F1-like ATPase-associated subunit", "uniref50_id": "UniRef50_UPI0034D97E37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D97E37", "UniRef:UniRef50_UPI0034D97E37" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D97E37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D97E37" ], "product": "MSC_0620 family F1-like ATPase-associated subunit" } }, "id": "DFOKDJOJIG_749", "locus": "GCA013367855_00737" }, { "type": "cds", "sequence": "contig_1", "start": 417644, "stop": 417745, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAACGTATATAAAATTGAATTACTTTTTTTAGAAAATAAGAAGTTAAACATCGAAAAAGGCTTGGTTTATATTAACGTTAATGAAGAAAATACATGA", "aa": "MNNVYKIELLFLENKKLNIEKGLVYINVNEENT", "aa_hexdigest": "c3ec65c564742151077d47bea6c19336", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3940.5179000000016, "isoelectric_point": 5.062103462219238 }, "id": "DFOKDJOJIG_750", "locus": "GCA013367855_00738" }, { "type": "cds", "sequence": "contig_1", "start": 417738, "stop": 418226, "strand": "-", "frame": 2, "gene": null, "product": "MSC_0622 family F1-like ATPase gamma subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR95", "UniRef:UniRef90_D1J8A4" ], "nt": "TTGCAATCTTATGAAAATTCAAATATTGAAAATTTAGCAGAAAAATTAACCAAAATAGTAATGATCTTGTATGAATATGAAAATTACGAAGAAGTCAATTTCGTAGTTAACTCCAATAAAAATTTTAATCAATATTTTACAATTTTGCCAATGTCTAAATTCTCGTTTGAAAAATTTCCTTTAAATGAAAGCAATAATAATTTATATGACATAGATTTTAAAAAAGCAAAAATATACCCTAATATTAATGATTTTATTAATTCTCAAATTAATTGTTATTTATTAAACACTATAAATATGCTAATATCCGAATCTGCATTTTATAAAGCAAAAATAGGTTTAGTTTCAACTAATACAATTTTAAAAGATTTAGACGAAAGCATTCTTAAACTTAAAAAGCAAATATCAAGAGCAAAAAGTGAATTGCAAATTGAGGAACTAAATTTATTGACTAAAAAAGACCCTGATGAAGGTGATATTTATGAATAA", "aa": "MQSYENSNIENLAEKLTKIVMILYEYENYEEVNFVVNSNKNFNQYFTILPMSKFSFEKFPLNESNNNLYDIDFKKAKIYPNINDFINSQINCYLLNTINMLISESAFYKAKIGLVSTNTILKDLDESILKLKKQISRAKSELQIEELNLLTKKDPDEGDIYE", "aa_hexdigest": "78276d3a930eca3700b485055d598886", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 417738, "stop": 418526, "observations": { "mutation": [ 418379 ], "stop": [ 418379 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8A4", "query_cov": 1.0, "subject_cov": 0.5436241610738255, "identity": 0.9940000000000001, "score": 309.0, "evalue": 2.87e-104, "gene_start": 1, "gene_end": 162, "reference_start": 137, "reference_end": 298, "reference_sequence": "MQLKDLENKRKSLNNISLKVNNEKNIYLINIMKLNQRLSFFNKNALVSRQIILHLKEIYNIKNDMLRRESRHRFNLFRKINRNILWIYLTEEQKYSTDSYSRYEKTILSNIKKSNNEFILIGKNAIKFGQDNNLQVLQSYENSNIENLAEKLTKIVMILYEYENYEEVNFVVNSNKNFNQYFTILPMSKFSFEKFPLNESNNNLYDIDFKKAKIYPNINDFINSQINCYLLNTINMLISESAFYKAKIGLVSTNTILKDLDESILKLKKQISRAKSELQIEELNLLTKKDPDEGDIYE" }, "paralog": false, "identity": 0.9923664122137404, "score": 509.0, "evalue": 1.68e-185, "description": "Internal stop codon at 418379. Nonsense mutation around 418379.", "psc": { "uniref90_id": "UniRef90_D1J8A4", "product": "MSC_0622 family F1-like ATPase gamma subunit", "uniref50_id": "UniRef50_Q9PR95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8A4", "UniRef:UniRef50_Q9PR95" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR95" ], "product": "Uncharacterized protein UU050" } }, "id": "DFOKDJOJIG_751", "locus": "GCA013367855_00739" }, { "type": "cds", "sequence": "contig_1", "start": 418377, "stop": 418634, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTAAAAGATTTAGAGAATAAACGCAAAAGTTTAAACAATATTTCATTAAAAGTAAATAATGAAAAAAATATTTATTTAATAAATATTATGAAACTTAACCAAAGATTAAGCTTTTTTAATAAAAATGCATTGGTATCAAGGCAAATAATTTTGCATTTAAAAGAAATCTACAATATTAAGAATGATATGTTGAGGAGAGAAAGTCGCCATAGGTTTAATTTATTTAGGAAAATAAATAGAAACATATTGTGA", "aa": "MQLKDLENKRKSLNNISLKVNNEKNIYLINIMKLNQRLSFFNKNALVSRQIILHLKEIYNIKNDMLRRESRHRFNLFRKINRNIL", "aa_hexdigest": "046d97bb3379b92a8896eac11c6d5c80", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10438.226399999985, "isoelectric_point": 11.068786430358884 }, "id": "DFOKDJOJIG_752", "locus": "GCA013367855_00740" }, { "type": "cds", "sequence": "contig_1", "start": 418636, "stop": 419139, "strand": "-", "frame": 1, "gene": null, "product": "DUF2714 domain-containing protein", "db_xrefs": [ "RefSeq:WP_012855593.1", "SO:0001217", "UniParc:UPI0001BC22B8", "UniRef:UniRef100_D1J8A5", "UniRef:UniRef50_D1J8A5", "UniRef:UniRef90_D1J8A5" ], "nt": "ATGAAAAAGAAAGACAATAATCCATTATTGAGTTATTTTGATGTATTTAAAGAATATAAAGAACACGAGTCCTCACAAGATTTAATTACTTTTGATGAAATGATATCAACAGTATTGTTTAATAACAACCTAGGATTTAATTCTCCTATTTATTTAGATTTTATAAGCAAAATTGATTTGGCTTTTAAAAATAAATATGACATTTTACTAAAGGATTTTAATATTACTTTTAATTTGAATTTGAAATTCTCGCAAGAGGTATTAGTACCTATAATTACAACAAAAAATTATTCTGCCAATAACGCAATTGATTTAACCACTTCTAGCGATATAAAATACCAAGAATTTTTGAATTCATATAATGCTGAAATAAAAAAACTTTTGGCCCTTAAAAAATCAATTAAAATATTTCCACATGTAATTTTATTTCATTCAGAATTAAAAAATGATTTAGTTTTATTATTCAGTGAAAAAGTAGTAGCAGTTGTTACAAAGGAACAATAA", "aa": "MKKKDNNPLLSYFDVFKEYKEHESSQDLITFDEMISTVLFNNNLGFNSPIYLDFISKIDLAFKNKYDILLKDFNITFNLNLKFSQEVLVPIITTKNYSANNAIDLTTSSDIKYQEFLNSYNAEIKKLLALKKSIKIFPHVILFHSELKNDLVLLFSEKVVAVVTKEQ", "aa_hexdigest": "95dd10e2a863166c6bec227d50a8595b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22B8", "ncbi_nrp_id": "WP_012855593.1", "uniref100_id": "UniRef100_D1J8A5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22B8", "RefSeq:WP_012855593.1", "UniRef:UniRef100_D1J8A5" ] }, "ips": { "uniref100_id": "UniRef100_D1J8A5", "uniref90_id": "UniRef90_D1J8A5" }, "psc": { "uniref90_id": "UniRef90_D1J8A5", "product": "DUF2714 domain-containing protein", "uniref50_id": "UniRef50_D1J8A5" }, "pscc": { "uniref50_id": "UniRef50_D1J8A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8A5" ], "product": "DUF2714 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_753", "locus": "GCA013367855_00741" }, { "type": "cds", "sequence": "contig_1", "start": 419120, "stop": 419551, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTAGCAATTGTTCAAATAGTGCTTGCGTTAAGTTTGCTATTGACTACATTATTTAACACCAATAGCATTGTTTCTTTAATCAACGTTGCTGTAACGGCAACAACACAAATAGTAATATTATTTGTGTTTGCTAAACAAAACAAGAATATTTCATTGCTATTATTAATAATAATTAAACTAGCAATAATCTCTTCAATTTTAATCTTTGCAATTATTGGATTTAATTATATTTTAACTGCTGATGGCAATGAAAATTATTTATTTTCAATAATTAAAAGTAGAATGAATATTATTCAAATTATCTTGCTTTTAAACTTTAGCATTGCATTTATTATGGCATTATCTTTATTGCTTAAATTTATTTTGGCAATTTATTATATAAGAAAATTAAATAAGGAGAATGAATATGAAAAAGAAAGACAATAA", "aa": "MNLAIVQIVLALSLLLTTLFNTNSIVSLINVAVTATTQIVILFVFAKQNKNISLLLLIIIKLAIISSILIFAIIGFNYILTADGNENYLFSIIKSRMNIIQIILLLNFSIAFIMALSLLLKFILAIYYIRKLNKENEYEKERQ", "aa_hexdigest": "4be300c372821fbb177876c2d1b67b51", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16209.493399999987, "isoelectric_point": 9.646098136901855 }, "id": "DFOKDJOJIG_754", "locus": "GCA013367855_00742" }, { "type": "cds", "sequence": "contig_1", "start": 419723, "stop": 419947, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGTAGGCAATAAATTTTATGAAACAATTAGAGATATTTTAACAATTAAATCTGCTAGCAATTTTGTGATTATTGTATTGACATTTTTAGTTTTAATTACTTTTATAGTATTATCTAATATTAAATTCTTTGCTTTATTGATAAATAAAAAATATGATAAGTTCTATTTAAAAAATCAATTACTATTTTTATTAGTATTATTTGGGGCTGTATTTATTTGA", "aa": "MFVGNKFYETIRDILTIKSASNFVIIVLTFLVLITFIVLSNIKFFALLINKKYDKFYLKNQLLFLLVLFGAVFI", "aa_hexdigest": "743c1e88d16c38a31320c8d39d0fcea8", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8658.491099999996, "isoelectric_point": 9.822999382019042 }, "id": "DFOKDJOJIG_755", "locus": "GCA013367855_00743" }, { "type": "cds", "sequence": "contig_1", "start": 419975, "stop": 420217, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTCATTAACTTCTTATACATTATTTTTTAGCTATTTTAAAATTCTTCCAAGCAAATTATTTGCAATATCATTTATTTTGATTGCTTTATATATTATTAATTTTAGCTATGAAATATTTAACTTTTTAATTCAAAGAAAAACAAATCCATTGCTTTATAAGAATAAAAATATTTTAATAATTTCTTTAACATTTCAAGGAATCTTAACAATATTATTTGTTGCAATATCATTTGTTTGA", "aa": "MLSLTSYTLFFSYFKILPSKLFAISFILIALYIINFSYEIFNFLIQRKTNPLLYKNKNILIISLTFQGILTILFVAISFV", "aa_hexdigest": "cd0216d165248626dfe7c9cb85b8c6b8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9370.249799999992, "isoelectric_point": 9.822999382019042 }, "id": "DFOKDJOJIG_756", "locus": "GCA013367855_00744" }, { "type": "cds", "sequence": "contig_1", "start": 420266, "stop": 420601, "strand": "-", "frame": 3, "gene": null, "product": "MSC_0624 family F1-like ATPase-associated membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E41E527", "UniRef:UniRef90_UPI001E41E527" ], "nt": "ATGAAACAAAAACAAATCGATTCGAACAATATAAATCAAAAGTTGATTTATGATGATGAATATTCAATAAAAAATCAGCAGAAAAACAACATTTTCAATAAGGTGTTGAAATACCTTTTGATTGTAATTAGTCTTGTATTTATTACAACTTTATTAATTTTTAGTGAAAAGACACTTTTTGCTAATAAAATGTTGCTTTCTAATTCACTAAATTCATTTTTTGACTTTTCAAGTCCAAGAAATCAACAATTAAACTTTTTAATAATTTATAGATTCTTAATTTTAGCCTTTGTCTTATTTTATAGTTTAGTAAAAGGCTTTGCTAATTTATATTGA", "aa": "MKQKQIDSNNINQKLIYDDEYSIKNQQKNNIFNKVLKYLLIVISLVFITTLLIFSEKTLFANKMLLSNSLNSFFDFSSPRNQQLNFLIIYRFLILAFVLFYSLVKGFANLY", "aa_hexdigest": "308b2720fad16246e333044276c711ea", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 419963, "stop": 420601, "observations": { "mutation": [ 419977, 420268 ], "stop": [ 419977, 420268 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E41E527", "query_cov": 1.0, "subject_cov": 0.4036363636363636, "identity": 0.973, "score": 194.0, "evalue": 2.09e-60, "gene_start": 1, "gene_end": 111, "reference_start": 1, "reference_end": 111, "reference_sequence": "MKQKQIDSNNINQKLIYDDEYSIKNQQKNNILNKVLKYLLIVISLVFITTLLIFSEKTLFANKMLLSNSLNSFFDFSSPRNQQLNFLIIYRFFILSFVLFYSLVKGFANLYWHKVTIKKYIPFYFLYLLLSLTSYTLFFSYFKILPSKLFAISFILIALYIINFSYEIFNFLIQRKTNPLLYKNKNILIISLTFQGILTILFVAISFVWINLGINKDILFVGNKFYETIRDILTIKSASNFVIIVLTFLVLITFIVLSNIKFFALLINKNMINSI" }, "paralog": false, "identity": 0.9764150943396226, "score": 397.0, "evalue": 9.75e-143, "description": "Internal stop codon at 419977, 420268. Nonsense mutation around 419977, 420268.", "psc": { "uniref90_id": "UniRef90_UPI001E41E527", "product": "MSC_0624 family F1-like ATPase-associated membrane protein", "uniref50_id": "UniRef50_UPI001E41E527", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E41E527", "UniRef:UniRef50_UPI001E41E527" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E41E527", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E41E527" ], "product": "MSC_0624 family F1-like ATPase-associated membrane protein" } }, "id": "DFOKDJOJIG_757", "locus": "GCA013367855_00745" }, { "type": "cds", "sequence": "contig_1", "start": 420617, "stop": 421018, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGGGATTAGAATTTCATGATAAATTAAAACCAAGTAATTCAAAATCAAGAAAGATAAGAAGACTTACTTTATATAATAACAGTGAAGCATTCAACATATCAGGTGAAGAATTAAATGAAGCAAGTTTTAATAGACATATTGTTTCAGGCGAACCTGGTCAAAAGAGCAAGATTTTATTTAGCAATGGAAATTTAACAACAAAAGTAAGAATTACGGGAACTTCATTACTAAATTCAGAAGGAATTGACAACCTTTCGCGTTTATATATGTTTGCAGAACAATTGAAAAAAGAAATAATTGTTGATAATAATGCCTCAGCTTTAAAATCTCAATTAGAAGGTTTGGGATATAGTGTAAAAACCTCTTCTTCTTCTGATTCTGACGGAGAATTTATTTAA", "aa": "MRGLEFHDKLKPSNSKSRKIRRLTLYNNSEAFNISGEELNEASFNRHIVSGEPGQKSKILFSNGNLTTKVRITGTSLLNSEGIDNLSRLYMFAEQLKKEIIVDNNASALKSQLEGLGYSVKTSSSSDSDGEFI", "aa_hexdigest": "c3c1a1db625c3fc57fd25b62a9718fe1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14760.36199999998, "isoelectric_point": 8.890786552429201 }, "id": "DFOKDJOJIG_758", "locus": "GCA013367855_00746" }, { "type": "cds", "sequence": "contig_1", "start": 421349, "stop": 421534, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAATCTGACTCTAGCCAAAAATTTATGGATATTTTAAAGGCAATTCCTGATGAAATTCAACTACTAGAACTATTTTTCTCAGCAGGTTCTTCAAACACTTCTTCTTTAATTGCATTAGAAAATAAACACATAAAGGAACTAGGAATTTATACTTTAGGAAATTCATTATTGGATAAATGA", "aa": "MGKSDSSQKFMDILKAIPDEIQLLELFFSAGSSNTSSLIALENKHIKELGIYTLGNSLLDK", "aa_hexdigest": "f763aab2bca3814fe56dea6fe263b2ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6701.625999999998, "isoelectric_point": 5.082394981384277 }, "id": "DFOKDJOJIG_759", "locus": "GCA013367855_00747" }, { "type": "cds", "sequence": "contig_1", "start": 421760, "stop": 422026, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCTCATTTAGATTTCTCTAAATTTACAAAGATTACTTCTAATGCTGAAAAGCAATTAGCTGAAGGCCTTGCATTAGATCAAGATAACGCCTATGTAAATGAAAAAGGTGAGCTAGATTCATATGCATATTCTCCAGCCAAAGGCTATAACGGTGTTGCAAATAGAATAATAAATGACAATAAAAATAGAAGAGTTTTTGGATATGATAGTATTGAAAATAGAAGCCCATATGGTCTTGCTAATGGCGACTATCCTGGTTGA", "aa": "MYSHLDFSKFTKITSNAEKQLAEGLALDQDNAYVNEKGELDSYAYSPAKGYNGVANRIINDNKNRRVFGYDSIENRSPYGLANGDYPG", "aa_hexdigest": "a74f8834f7055dc56c4ed59ec082d28e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9832.597499999996, "isoelectric_point": 5.229266929626463 }, "id": "DFOKDJOJIG_760", "locus": "GCA013367855_00748" }, { "type": "cds", "sequence": "contig_1", "start": 422234, "stop": 422911, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACTTATTAAAGAAAAGAAAAAACAAATTAATTTTAATGACAGCAGGTGTTATTATAGGAGCTTCAATAATTGCTGGGGCTATATTTTATAGTACAAGTTCGCAAAATTATTCTCGTTTTAATACCACCAGCTCAGCAACAGGAAATGATTTTATAAATAAAAGCAACCTAGATTATAAAAATGCGTTGCCTTCAATAAAGGATAACAACTTAAAAGAATTAGAAAAGCCACAACCAATTCCAACCCCTAAACTAGAACCTGAACCAATCCCAGTTCCGCCAGCTCCAAAACCAGAGCCAAAACCCGAACCCAAACCAGAACCAAAACCTCAACCAACCCCTCCAGCTCCAAAGCCAGAACCAAAACCCAAACCTGAACCCAAGCCTACTCCTCCTTCATCAAAGCCTTCAACTAGAACAACTGAAATTATTCTAAACGGTGTCAAAGTTGAAGCGGAAGTCGAAGATCAAAAGAAGCGTGAATATAATCAATATGATATCTTAAATCATCTAACTAATTTAAACCCATACATCAATGATAGTGCTCCAAAAATTAAAAAAGTTAAGGTTACTGAAGAATTGAAACAAGCAGCAGCCCAAGCGGCTACTCAACGTTTAAAAAGAGATGAAAAATACTTCAAAAAAGATCTTGAAGATTATGAAAAAAGTTGA", "aa": "MKNLLKKRKNKLILMTAGVIIGASIIAGAIFYSTSSQNYSRFNTTSSATGNDFINKSNLDYKNALPSIKDNNLKELEKPQPIPTPKLEPEPIPVPPAPKPEPKPEPKPEPKPQPTPPAPKPEPKPKPEPKPTPPSSKPSTRTTEIILNGVKVEAEVEDQKKREYNQYDILNHLTNLNPYINDSAPKIKKVKVTEELKQAAAQAATQRLKRDEKYFKKDLEDYEKS", "aa_hexdigest": "cebf1fac198b19c8df8f6e680df31c7f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25210.621300000013, "isoelectric_point": 9.507748985290526 }, "id": "DFOKDJOJIG_761", "locus": "GCA013367855_00749" }, { "type": "cds", "sequence": "contig_1", "start": 422942, "stop": 423418, "strand": "-", "frame": 3, "gene": null, "product": "MIP family Ig-specific serine endopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "nt": "GTGTTTTTAGCACAACCAGGTGGAACCAAGCAAGTTCAAGAAGAAATAAATGGCCAAAAATATCAAAAATCAGTATTTTCATTCTATGAAAGTAGCGATGGCAAAACATATATGCGTATGGGGCTAAATTATATGCCTAAACATTTTGCTCCAGTTGGAGGTGCTTCTGGTACAAGCTTAAGAAATCAAAAAAACGAACTTGTTGGAATATTTTATTTATCGAATTTAGGCGCAAAAACCGGCTTAGCAGCTGCTTTTAGATCGGAAGGATTTGACTACAAAGGCTTGTATGGAAATTACAATTTGCCTCAATATGATCTAATTTATGGTGGCGGCAAAGACCAAAAAAATTCATTTAGACAAACATTAAAAGAACTATATAAAGATAATAGTGATTTTAAAACAGCTTTATTCCCTGATGGCTTAGGTGATGATAAAGTTCCTAATGAATTTAAATTTATAGAAAACAAAAAATAA", "aa": "MFLAQPGGTKQVQEEINGQKYQKSVFSFYESSDGKTYMRMGLNYMPKHFAPVGGASGTSLRNQKNELVGIFYLSNLGAKTGLAAAFRSEGFDYKGLYGNYNLPQYDLIYGGGKDQKNSFRQTLKELYKDNSDFKTALFPDGLGDDKVPNEFKFIENKK", "aa_hexdigest": "de97ec5808ecf6bbdeffb285b0149992", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DFAD80", "query_cov": 0.8354430379746836, "subject_cov": 0.8048780487804879, "identity": 0.5760000000000001, "score": 162.0, "evalue": 2.5e-48, "valid": false, "product": "MIP family Ig-specific serine endopeptidase", "uniref50_id": "UniRef50_UPI0021DFAD80" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFAD80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "product": "MIP family Ig-specific serine endopeptidase" }, "genes": [], "id": "DFOKDJOJIG_762", "locus": "GCA013367855_00750" }, { "type": "cds", "sequence": "contig_1", "start": 423689, "stop": 424144, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGGATAGTGCAAAAATTAAAACAACATTGCGTTTAGCAAATCTTGATGAAAATACATATAGATTTTCATTTGTTGGTAAAGATTCTACTAATGTTTCTAATGGAATAAAGACAGTTTATACTGCAAGAGATTTCTTAAATTCAAAACCTTCTGATTATTTAATTAAATCGCAAAAAGAAAAGTATAAAGATGTTGAAGAATATGCTGATTTTGCAGTCTTTGAAGTTGATTTTGAAAAATTGACATTGCAAAGCGTTTCTAAAAATGATCTAAGTGAAAATAGCAATATTACACAAAAATTTAGCAATTTAGATGCCCACGAATTAGCAAAAGCCTTAACAAGTAATTATGCTCAAAATAAAGCAAAACAAATTAAATTCTTGTCGAAATCATATTTATCAAATTATCAAAAAATTGATGTTCCTTTAAAAGCAAATAAAGAAAACAAGGAATGA", "aa": "MDSAKIKTTLRLANLDENTYRFSFVGKDSTNVSNGIKTVYTARDFLNSKPSDYLIKSQKEKYKDVEEYADFAVFEVDFEKLTLQSVSKNDLSENSNITQKFSNLDAHELAKALTSNYAQNKAKQIKFLSKSYLSNYQKIDVPLKANKENKE", "aa_hexdigest": "c1196c208f62d50fbb005a86b13f86a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 149, "aa_cov": 0.9271523178807947, "hmm_cov": 0.8322147651006712, "evalue": 1.4656867252406123e-25, "score": 86.23676300048828, "start": 2, "stop": 141 } ], "seq_stats": { "molecular_weight": 17302.1768, "isoelectric_point": 8.872670936584473 }, "id": "DFOKDJOJIG_763", "locus": "GCA013367855_00751" }, { "type": "cds", "sequence": "contig_1", "start": 424265, "stop": 424873, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTTAATATTACTATTAAAGACAAAAAGACAAACAAAACATTTATTGAAACAATAACTGTAGGAAAATTTAAAACAAATCCTTATGGTTTTGATGAAAATGGAACAATTCCTAATACAGGTTCTGATGCTTTAAGATTGAATAAAAGTGAGTTGGAACAATATCTTGAAGGAAATCAAACAAAAAGATATGAGCTAGATAATAAAAAATATTTAGACATTTTAACTCGTCAACAAGAACAAAAATCAATAAAAGAAATTAGACCAAATTTGGAAGCTTCACAATCTAACATTCAAAAATTTGATGCTAAGGCAAAAGAAATTAGTCAAGATTCGTATGAATCAGCTGCAAGAAAAGGTTTTACATTGCCAAGCTACGATTCAAATGGTAATGTAGAAGGCCTTGCTATTAATGAAACTCCAGGAGGACAAGGGCCTTCACCTACAGATGCATTTGGTAGAGATCCATATAAAATTACAGGTTTAGCAAGAACATTGCCAAATGAACATTATCGTGACATTGCTTCTCAAACATTTGGAATAAGATTCTTAACTCCTATTAAAGGTGAAGAAAGAAAATTTACTAATACAGCTGGAACAACTTGA", "aa": "MNFNITIKDKKTNKTFIETITVGKFKTNPYGFDENGTIPNTGSDALRLNKSELEQYLEGNQTKRYELDNKKYLDILTRQQEQKSIKEIRPNLEASQSNIQKFDAKAKEISQDSYESAARKGFTLPSYDSNGNVEGLAINETPGGQGPSPTDAFGRDPYKITGLARTLPNEHYRDIASQTFGIRFLTPIKGEERKFTNTAGTT", "aa_hexdigest": "c6295834f315fb2c42cc70eda68671c3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22699.937899999994, "isoelectric_point": 8.596037101745608 }, "id": "DFOKDJOJIG_764", "locus": "GCA013367855_00752" }, { "type": "cds", "sequence": "contig_1", "start": 424898, "stop": 425368, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAATCTAAATTAATAATATCATTATTAATAGCAGCGACATCACTTTCTAGTATGTCTGCTGTAGCTATTTCATGTAAAAATACAAATAAAAAGACTAATAATAATCAAAATGACGAAAAGAAGAATCCTGATGAAAAACCCCAAAATAATCAAGATTCAAAAGGCAATGACAACAAAAATAATAATTCAACAGATGAACTATCAGCTGAAGAACAAAAAACTATCGAATATAAAAAAATAATTTCTGAAGTTTCAAAATTAGCATTAAATGATCCAAGTCAATTATTTGATTTGAAATTCAAGGCTTTTTCAAATCAAAATTCACAAGATTTGCTTCCATCTCAAATTGTTAGAAATTTCAATCAAGGTGTTGAGTTTAAACAAAAAAAATTTAATGATGAAATTGAAATTAAAAAAATAGTCAATGTTTTATACGTACAAACTTCAACCAATTCTGATGATTGA", "aa": "MKKSKLIISLLIAATSLSSMSAVAISCKNTNKKTNNNQNDEKKNPDEKPQNNQDSKGNDNKNNNSTDELSAEEQKTIEYKKIISEVSKLALNDPSQLFDLKFKAFSNQNSQDLLPSQIVRNFNQGVEFKQKKFNDEIEIKKIVNVLYVQTSTNSDD", "aa_hexdigest": "08cf25cb6f5d320f7fcb1d726abddf86", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17617.4647, "isoelectric_point": 8.525508689880368 }, "id": "DFOKDJOJIG_765", "locus": "GCA013367855_00753" }, { "type": "cds", "sequence": "contig_1", "start": 425681, "stop": 426067, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_V8AMI9" ], "nt": "ATGTTGGATGTTTGCAATTTAGCTAAAGTTTTTCCAGACAAAAAATTATTTACAAATGTTAACTTAAAATTCTTGCCTGGAAATGTATATGGAATTATTGGCGCTAATGGGGTTGGAAAATCCACTTTTTTAAAAATATTAAGTGGGGATATTGAAGCATCAGAAGGGCAAATAATTAAAGAAAAAAATGCTAGAATGAGTGTATTATCACAAAACCAAAATGAATTCGACGATTATATTGCAACTGATGTTGTAATAATGGGGAACAAAGAACTTTATCAAATTAATCTTGAAAAAACTAAAATTTATGAAGATCCAAATGCAACTATTCAAGATTATGAAAAAGCAAGCCATTTAGAAGAAAAATTTGGTGAAATGGGGGGTTGA", "aa": "MLDVCNLAKVFPDKKLFTNVNLKFLPGNVYGIIGANGVGKSTFLKILSGDIEASEGQIIKEKNARMSVLSQNQNEFDDYIATDVVIMGNKELYQINLEKTKIYEDPNATIQDYEKASHLEEKFGEMGG", "aa_hexdigest": "c70b676939e47c862fa354f4f78ad32e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0011CB74F5", "query_cov": 0.8359375, "subject_cov": 0.9727272727272728, "identity": 0.514, "score": 108.0, "evalue": 2.01e-28, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_V8AMI9" }, "pscc": { "uniref50_id": "UniRef50_V8AMI9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_V8AMI9" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_766", "locus": "GCA013367855_00754" }, { "type": "cds", "sequence": "contig_1", "start": 426131, "stop": 426259, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGTCGGATTTAAAAGCCAATGAAAAAGTAAAGGTTTTATTGGCAAAGGCTTTGTTTGGAAATCCTGATATTTTAGTAATGGATGAACCAACAAACAGATTGGATCTAAAAACAATTAAGTGA", "aa": "MKMSDLKANEKVKVLLAKALFGNPDILVMDEPTNRLDLKTIK", "aa_hexdigest": "767e909f7a3a553f1973341b68c98a0f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4742.6662, "isoelectric_point": 9.22402362823486 }, "id": "DFOKDJOJIG_767", "locus": "GCA013367855_00755" }, { "type": "cds", "sequence": "contig_1", "start": 426275, "stop": 426400, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTATCCAAACATTGTTATTGTTGTTTCTCACGATAGTGATTTCTTAGATGCAATTTGCACGCATATTATTGACATAGATTATTCTGAAGCTAAATTATTTATTGGAAATTATTCATTTTGA", "aa": "MDYPNIVIVVSHDSDFLDAICTHIIDIDYSEAKLFIGNYSF", "aa_hexdigest": "459d1e9fe7647d18e60ab69fb0c47a61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4695.236499999998, "isoelectric_point": 4.0500284194946286 }, "id": "DFOKDJOJIG_768", "locus": "GCA013367855_00756" }, { "type": "cds", "sequence": "contig_1", "start": 426428, "stop": 426817, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33", "PFAM:PF12848.13" ], "nt": "ATGCAACAAAAACAAAACCTTAAAAAAGAAGAAATTGCCCAAAAATTAAAAGATTTTATTGCCAGATTCTCTGCTAATGCCTCAAAATCAAAACAAGCTACAAGTAGAAAGAAATTACTTGATAAAATTGTCATTGATGAAATCAAACCATCATCTAGAAAATATCCTTTCATTCAATTTAACTTAAATAGAACTCCTGGAAAACAAATATTAACTGTTAAAAATTTAACTTATGTAAATGACAATGGCGACACTTTATTTGAAAATGTAAGTTTTACAATCCACCCCGGAGAAAAAATGGTTATTATTGGCAATGACGACATTGCCAAAACTAGATTGCTTGAAATACTTATGGGTAAGCGTAAACCAACATCAGGAACAGTTGAATGA", "aa": "MQQKQNLKKEEIAQKLKDFIARFSANASKSKQATSRKKLLDKIVIDEIKPSSRKYPFIQFNLNRTPGKQILTVKNLTYVNDNGDTLFENVSFTIHPGEKMVIIGNDDIAKTRLLEILMGKRKPTSGTVE", "aa_hexdigest": "a837073c1b585038c050706acd779c73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 41, "aa_cov": 0.3178294573643411, "hmm_cov": 1.0, "evalue": 7.989928912655033e-08, "score": 28.639638900756836, "start": 88, "stop": 128 }, { "id": "PF12848.13", "name": "ABC_tran_Xtn", "length": 59, "aa_cov": 0.4573643410852713, "hmm_cov": 0.9661016949152542, "evalue": 5.022977732353303e-13, "score": 44.65205001831055, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 14693.900499999978, "isoelectric_point": 9.908742332458495 }, "id": "DFOKDJOJIG_769", "locus": "GCA013367855_00757" }, { "type": "cds", "sequence": "contig_1", "start": 426956, "stop": 427294, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGCATTTTTAGGAAGAATGTTATTTTCAAATGATAGCGTATTTAAGAATGTTAATGTAACTAGCGGTGGTGAAAAAGTTAGATTAATGATGGCTAAACTAATGTTAGAAGAAAGTAACTTTTTAATCTTCGACCAACCTCTTGACCACCTTGATAGTGAATCAATTGATAGTTTAATTGAAGCAATTAAAAATTACAAAAGTAGTTGTATATTCACAACATACAATAGAGCAATGATAAAAGAATGTGCCTCAGTTATTTTAGAAATAAAACCTACTTCAAGTTATTTATTCTATGGAACCTTAGAAGAATATGAAGAAGCAATGGGATATTAA", "aa": "MRAFLGRMLFSNDSVFKNVNVTSGGEKVRLMMAKLMLEESNFLIFDQPLDHLDSESIDSLIEAIKNYKSSCIFTTYNRAMIKECASVILEIKPTSSYLFYGTLEEYEEAMGY", "aa_hexdigest": "09499d814b522967d8a94203f3f165be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12834.661299999987, "isoelectric_point": 4.819230461120605 }, "id": "DFOKDJOJIG_770", "locus": "GCA013367855_00758" }, { "type": "cds", "sequence": "contig_1", "start": 427663, "stop": 428253, "strand": "-", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_D1J8W0", "UniRef:UniRef90_UPI00273707BF" ], "nt": "GTGTGTGGAATATTGAGTGTTCTAAATAAATCGAAATTAAATGTGAAATATTTAAGCACTTGCTTATCTATGCAGACAAAAAAATTTGTAAACAAGGCAAGTGGAAATCCTAAACTTATGAGCAATATTATGGAAAATATTGAAATTCCTATCCCTCACATTTCAATCCAAAACAAAATAGTTGAAATCCTTGACAAACTTGAAACATATACCAAAGACATTCAATCCGGCCTTCCTCTTGAAATTGACCAACGCAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTTAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTACATATTATTATTAAATAAACTTTATGAAAAGATAATTAACATTATTGAATATAAGAGGATAAATGAGGTAACTATTAATTTAAAAAAGGAAACATTAGAAAAAAACAAATTACTAAATAATGGCAAATATCAAGTAATTAATAGTGGCAAAGAAATATATGGGACATATGACCAATATAATAATGAAGGAAATGCTATAACAATTGCAGCGCGTGGAGCATATGCTGGTTTTATAAATTATATGAATGATAAATTTTGA", "aa": "MCGILSVLNKSKLNVKYLSTCLSMQTKKFVNKASGNPKLMSNIMENIEIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGVLSKNYILLLNKLYEKIINIIEYKRINEVTINLKKETLEKNKLLNNGKYQVINSGKEIYGTYDQYNNEGNAITIAARGAYAGFINYMNDKF", "aa_hexdigest": "419e0462f179ef783868540333956cf7", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 427360, "stop": 428553, "observations": { "mutation": [ 427665, 428319, 428511 ], "stop": [ 427665, 428319, 428511 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00273707BF", "query_cov": 1.0, "subject_cov": 0.47572815533980584, "identity": 0.9690000000000001, "score": 374.0, "evalue": 7.45e-128, "gene_start": 1, "gene_end": 196, "reference_start": 104, "reference_end": 299, "reference_sequence": "MRGGVLSKNYLLLLNELWDKIVNIVEYDLIKNLCEIKRGKVYSKEFIKLNKGEYPVYSSQSLNDGILGKIDKYDFDGEYVTWTTDGAYAGTVFYRIGRFSITNVCGILSVLNKSKLNVKYLSTCLSMQTKKFVNKASGNPKLMSNIMENIEIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGVLNNNYILLLNKLYEKIINIIEYKRINEATINLKKETLAKNKLLNNGKYQVINSGKEIYGTYDQYNNEGNTITIAARGAYAGFINYMNDKFWAGGLCYPYRSKNETSFLTKYIYYWLKYNEEKISNELVAKGSIPALNKIDIDNYFIPIPHISIQNKIVEILDKLQAYTKDIQTGLPLEIDQRKKQYEHYRDKLLNFKEKNTKI" }, "paralog": false, "identity": 0.9622166246851386, "score": 765.0, "evalue": 2.24e-284, "description": "Internal stop codon at 427665, 428319, 428511. Nonsense mutation around 427665, 428319, 428511.", "psc": { "uniref90_id": "UniRef90_UPI00273707BF", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_D1J8W0", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00273707BF", "EC:3.1.21.3", "UniRef:UniRef50_D1J8W0", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8W0" ], "product": "Type I restriction enzyme specificity protein" } }, "id": "DFOKDJOJIG_771", "locus": "GCA013367855_00759" }, { "type": "cds", "sequence": "contig_1", "start": 428317, "stop": 428466, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGTGTGAAATAAAGCGAGGAAAAGTTTACTCAAAAGAATTCATAAAATTAAATAAAGGTGAATACCCTGTTTATTCATCTCAAAGTTTAAATGATGGAATTTTAGGAAAAATTGACAAATATGATTTTGATGGTGAATATGTAACATGA", "aa": "MCEIKRGKVYSKEFIKLNKGEYPVYSSQSLNDGILGKIDKYDFDGEYVT", "aa_hexdigest": "b0f70c5bb8111e7c075e0d3502c6a325", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 53, "aa_cov": 1.0, "hmm_cov": 0.9811320754716981, "evalue": 2.4423843655236296e-09, "score": 33.23988342285156, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 5670.380600000001, "isoelectric_point": 6.121525382995604 }, "id": "DFOKDJOJIG_772", "locus": "GCA013367855_00760" }, { "type": "cds", "sequence": "contig_1", "start": 428509, "stop": 429063, "strand": "-", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0A454CAF7", "UniRef:UniRef90_UPI00193A7487" ], "nt": "TTGTTTGATATTATAATACCCCCTAAGAAAATACCTATTAACGAAACAATGAATAATGGAAAATATATAGTTATTAATCAAGGCGATGATAAAAAAATGTATACAAATGATGAAAATGCTGTTTTGCCAGCAAATGAATATATTTTGTTTGGTGATCATACAAGAAAAATTAAATTTATAAATCATACCTTTGCACAAGGTGGTGATGGTTTAAAAATTTTATTAAATAAAGATAAAACAAAAAATAATGTATCATCAAAATTTTTATTTTATTCATTAGATAATATTAATATACCTTCTTTAGGATATAAAAGACATTGGTCTTCAATAACGAATATCAAAATCCCCATCCCTCACATTTCAATTCAAAATAAAATTGTTGAAATTCTTGACAAACTTGAAACATATACCAAAGACATTCAATCCGGCCTTCCTCTTGAAATTGACCAGCGTAGAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAGCTTTGA", "aa": "MFDIIIPPKKIPINETMNNGKYIVINQGDDKKMYTNDENAVLPANEYILFGDHTRKIKFINHTFAQGGDGLKILLNKDKTKNNVSSKFLFYSLDNINIPSLGYKRHWSSITNIKIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRRKQYEYYRDKLLDFKDLAGGVLSKNYLLLLNEL", "aa_hexdigest": "a60fc8a720ffec41561ad1880c159663", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 428545, "stop": 429336, "observations": { "mutation": [ 429078 ], "stop": [ 429078 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193A7487", "query_cov": 0.9347826086956522, "subject_cov": 0.41445783132530123, "identity": 0.971, "score": 343.0, "evalue": 9.85e-116, "gene_start": 1, "gene_end": 172, "reference_start": 243, "reference_end": 414, "reference_sequence": "MNKIEKLIKELCPNGVEYKKIGEISTLFSGMKGVSKKWSEEGNCKFIDYMNCYKNFRIDVSLLKNATVKSLNQNILQKNDILFTASSETPDECCISSIIDDYIEDKIFMDDHLFAIRILKDFLKEINPIYLNFYFHCVDFRKKVNKIAKGMTRFYIPKSDFLNIQIPLPPLEIQNQIVNILDKFTELTTELTTELTYRDKQYNYYRNKLLDFDNNKNLIAKIMNKTTSNSLNNLVNYWKILDLFDIIIPPKKIPINETMNKGKYIVINQGDDKKMYTNDENAVLPANEYILFGDHTRKIKFINHIFAQGGDGLKILLNKDKTKNNVSSKFLFYSLDNINIPSLGYKRHWSSITNIKIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGIK" }, "paralog": false, "identity": 0.9049429657794676, "score": 462.0, "evalue": 8.91e-165, "description": "Internal stop codon at 429078. Nonsense mutation around 429078.", "psc": { "uniref90_id": "UniRef90_UPI00193A7487", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A454CAF7", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193A7487", "EC:3.1.21.3", "UniRef:UniRef50_A0A454CAF7", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454CAF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAF7" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "DFOKDJOJIG_773", "locus": "GCA013367855_00761" }, { "type": "cds", "sequence": "contig_1", "start": 429076, "stop": 429579, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGATAATAATGTCTTAAGAATAAAAAAAATTTTTAAAGCAAATTCAATTATATTATCTACTACCGCAACAATTGGTGAACATGCTATTGTATTAGAACCATTTTTTTGCAATCAACAATTTTCAATTTTTACTGTTAAAGATAAATTTGTGGCTAAAATAAACCCAATGTTTATGTTTTATTATTTTTTTATCTTTTCAAAAATCTTAAAGCAAAGATATGAAAACGTAAATTTACCTTTAATTAAAAAAGAGGAACTTGGGAATTTATTATTCCCCATTCCTAATATTTCAATCCAAAATCAAATTGTTAATATTTTAGACAAGTTCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCTATAGAAATAAACAATATAATTACTATAGAAATAAATTATTAGACTTTGATAATAATAAAAATCTAATAGCCAAAATTATGAATAAAACAACTTCAGATTCTTTAAATAATTTAGTTAATTATTGA", "aa": "MDNNVLRIKKIFKANSIILSTTATIGEHAIVLEPFFCNQQFSIFTVKDKFVAKINPMFMFYYFFIFSKILKQRYENVNLPLIKKEELGNLLFPIPNISIQNQIVNILDKFTELTTELTTELTTELTYRNKQYNYYRNKLLDFDNNKNLIAKIMNKTTSDSLNNLVNY", "aa_hexdigest": "64fcfec7569d918538bde82584977400", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 118, "aa_cov": 0.7065868263473054, "hmm_cov": 0.9661016949152542, "evalue": 3.2439083207378006e-18, "score": 62.12223434448242, "start": 9, "stop": 126 } ], "seq_stats": { "molecular_weight": 19702.69210000001, "isoelectric_point": 9.214546775817869 }, "id": "DFOKDJOJIG_774", "locus": "GCA013367855_00762" }, { "type": "cds", "sequence": "contig_1", "start": 429658, "stop": 429780, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAATAGAAAAGTTAATTAAGGAACTATGTCCTGATGGAGTTGAATATAAAAAAATGCAGGATATCTGCGACATATTTGGAGGTTTTACTCCCCAAAAAAATCTTTCCGAATATTGA", "aa": "MSKIEKLIKELCPDGVEYKKMQDICDIFGGFTPQKNLSEY", "aa_hexdigest": "4e192e7d66eef659240fb829cf0457b2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4640.377100000001, "isoelectric_point": 5.095865821838378 }, "id": "DFOKDJOJIG_775", "locus": "GCA013367855_00763" }, { "type": "cds", "sequence": "contig_1", "start": 429783, "stop": 430247, "strand": "-", "frame": 2, "gene": null, "product": "type I restriction-modification system subunit M", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0009307", "KEGG:K03427", "SO:0001217", "UniRef:UniRef50_A0A7X6YKI2", "UniRef:UniRef90_UPI001939D1E0" ], "nt": "ATGTATCGCGGGGGCGCTGAACAAAAAATTAGACAATATTTAGTCGAAAATAACTTCATAGATGCAATAATTCAACTCCCAAGCAACTTGTTTTTTGGAACAAGTATTTCAACATGCATAATGGTATTGAAAAAATCAAAAATTGACAACAATATCTTATTTATTGACGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAACAAGCTAACTAGTCAAAATATTAACAATATTATTGATTATTATGGCCAAAGAAAAGACATTTCTTATATTTCAAAATTAGCCAGTGTTGAAGAAATTAAGTCCAATTCATATAATTTATCAGTCAATAGTTACGTTGAAAAACAAGACACTTCAGAAAAAATAGATATTAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAACATTGAGAAGTGAAATTGACAAAATAGTTGCCGAATTAGAGGAATAG", "aa": "MYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQRKDISYISKLASVEEIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE", "aa_hexdigest": "02094b489d20e08f50d7311fa956909d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6V7S9", "query_cov": 0.9935064935064936, "subject_cov": 0.9807692307692307, "identity": 0.66, "score": 200.0, "evalue": 2.11e-63, "valid": false, "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A645HPD1" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 429783, "stop": 430547, "observations": { "mutation": [ 430295, 430406 ], "stop": [ 430295, 430406 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939D1E0", "query_cov": 1.0, "subject_cov": 0.4652567975830816, "identity": 0.9940000000000001, "score": 282.0, "evalue": 2.62e-93, "gene_start": 1, "gene_end": 154, "reference_start": 178, "reference_end": 331, "reference_sequence": "MYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIKWEGEDNPLLINDQRFSPAGILAPKSKADFAFILHSLSWLATDGVAAIVCFPGIMYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQWKDISYISKLASVEEIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE" }, "paralog": false, "identity": 0.9881889763779528, "score": 489.0, "evalue": 3.34e-177, "description": "Internal stop codon at 430295, 430406. Nonsense mutation around 430295, 430406.", "psc": { "uniref90_id": "UniRef90_UPI001939D1E0", "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7X6YKI2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939D1E0", "EC:2.1.1.72", "UniRef:UniRef50_A0A7X6YKI2", "KEGG:K03427", "GO:0003677", "GO:0009007", "GO:0009307" ] } }, "id": "DFOKDJOJIG_776", "locus": "GCA013367855_00764" }, { "type": "cds", "sequence": "contig_1", "start": 430404, "stop": 431231, "strand": "-", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "nt": "ATGTTTTATAGATATATTTCTGAAAATATTGCAACATATGCAAACAATCGCCAACGTCAAGCAGGAATTGAAGATTTTGATTATACAACTTTATCAGATGAAGAAACATTGACGGGCAAAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTACCTTCTGAATTGTTTATTAATGTTGTTAAAAATGCTTCAACAAACAATTGTTTAAATGAAACGTTGGACAATATTTTTAAAAATATTGAATCATCAGCAAAAGGACAACAAAGTGAAAATGATTTTTCAGGTCTATTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTGCAAGAAATAGCAGCCATGAAATTAGGCAATTATCAAGATAATTCAATAGATGCCTTTGGTGATGCCTATGAATATTTGATGTCAATGTATGCATCAAATGCTGGAAAATCAGGGGGTGAATATTTTACTCCCCAAGAAGTTTCTGAATTACTTACTAAAATAGCAGTTTTTAATAAGAAAAAAGTTAATAGAGTATATGACCCTGCATGTGGATCGGGTTCTTTACTTTTACAAACAATCAAGGTTCTTGGCAAAGAAAACATCAAGGATGGATTTTATGGCCAAGAAGTCAATTTAACAACATATAACTTATGTAGAATAAATATGTTTTTGCATGATATAGGATTTGATAAATTCAATATTTACAACGGCGACACATTGCTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTTATTGTTTCAAATCCTCCTTATTCAATTAAATGA", "aa": "MFYRYISENIATYANNRQRQAGIEDFDYTTLSDEETLTGKDDLINEKGFFILPSELFINVVKNASTNNCLNETLDNIFKNIESSAKGQQSENDFSGLFNDVDVNSQKLGRSVDERNKKLAAILQEIAAMKLGNYQDNSIDAFGDAYEYLMSMYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIK", "aa_hexdigest": "324bf9f0ba6b8c39a84a45ae0ad2af9e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028EAAB7E", "query_cov": 1.0, "subject_cov": 0.8136094674556213, "identity": 0.6509999999999999, "score": 364.0, "evalue": 1.8e-123, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7U9ST90", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9ST90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "DFOKDJOJIG_777", "locus": "GCA013367855_00765" }, { "type": "cds", "sequence": "contig_1", "start": 431538, "stop": 432092, "strand": "-", "frame": 2, "gene": "yajL", "product": "DJ-1/PfpI domain-containing protein", "db_xrefs": [ "COG:COG0693", "COG:V", "RefSeq:WP_044329558.1", "SO:0001217", "UniParc:UPI0005C92419", "UniRef:UniRef100_UPI0005C92419", "UniRef:UniRef50_D1J8B4", "UniRef:UniRef90_D1J8B4" ], "nt": "ATGAATTTATTAGTAATTGTTGAAGAAGGATTTGAAGATACTGAATTAACAGGAACATTAGATTTATTTCATAGAGCAAATAATTTATTTAATAAGACCACATTTTACAATCCTCTAGGAATTGAAGCAACTGGTGCACATAAAATTGTAAAATTGCAAACAACTTCAAATATAAATGTTGATGAATATGATGTTTTATTTATTCCTGGAGGACCCGCTGCTAAAAGTATTAGAAAGAACCAAACATTTTTAAACTTAATAGCTAAATTTAAAAGTCAAAACAAATTAATTATAGCAATATGCGATGCTCCCAATGTTTTATATGAAAATAAGTTTATTGATAGTAAAACTAAATTTAGTTCATTTCCAATAAACGACAGTGTAAAAGAAATATCTAATCGTAGTAAAGAATTAGTATCAATAAGCCCAGATAAAAAATTAATAACAGGAAAATGCGCGGCTGCTGTTTTTGATTTTGGATTAGCTGTTATTGAAGTTTTGGGTGGCAATCAACTTGCTAAATTAGTTCTTCAAGCAATAAAACCTATTGACTAA", "aa": "MNLLVIVEEGFEDTELTGTLDLFHRANNLFNKTTFYNPLGIEATGAHKIVKLQTTSNINVDEYDVLFIPGGPAAKSIRKNQTFLNLIAKFKSQNKLIIAICDAPNVLYENKFIDSKTKFSSFPINDSVKEISNRSKELVSISPDKKLITGKCAAAVFDFGLAVIEVLGGNQLAKLVLQAIKPID", "aa_hexdigest": "7f97d81814c7b6cd7dfae16d86fdc752", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0005C92419", "ncbi_nrp_id": "WP_044329558.1", "uniref100_id": "UniRef100_UPI0005C92419", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C92419", "RefSeq:WP_044329558.1", "UniRef:UniRef100_UPI0005C92419" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C92419", "uniref90_id": "UniRef90_D1J8B4" }, "psc": { "uniref90_id": "UniRef90_D1J8B4", "gene": "YajL", "product": "DJ-1/PfpI domain-containing protein", "uniref50_id": "UniRef50_D1J8B4", "cog_id": "COG0693", "cog_category": "V" }, "pscc": { "uniref50_id": "UniRef50_D1J8B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B4" ], "product": "DJ-1/PfpI domain-containing protein" }, "genes": [ "yajL" ], "id": "DFOKDJOJIG_778", "locus": "GCA013367855_00766" }, { "type": "cds", "sequence": "contig_1", "start": 432244, "stop": 432423, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF", "UniRef:UniRef90_UPI0015F0C8A9" ], "nt": "TTGCCTGGTTTATATATTAATATTCATAAATTAACAATTCATATGAATTCAAATCCTAAAACGATTGAAATGGATTTTAGCTTAAAATATTCAAAATCTAAAGAACATCAACAAAAGATTGAAGGTTTTGAAGATGACTATATTTTTGAATATAAATATATATTCAAAGAAAAAAAGTAA", "aa": "MPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK", "aa_hexdigest": "084e7b801b313560ac8c22a03bd1e288", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 432244, "stop": 432594, "observations": { "mutation": [ 432432 ], "stop": [ 432432 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "query_cov": 1.0, "subject_cov": 0.5086206896551724, "identity": 0.983, "score": 120.0, "evalue": 6.01e-34, "gene_start": 1, "gene_end": 59, "reference_start": 58, "reference_end": 116, "reference_sequence": "MFEAKFIDNENVDLKEAFFKYNSGYSNFKKTKVYANLKSGEITIKNFELGAAKDWKELPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK" }, "paralog": false, "identity": 0.9827586206896551, "score": 234.0, "evalue": 2.4e-81, "description": "Internal stop codon at 432432. Nonsense mutation around 432432.", "psc": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0015F0C8A9", "UniRef:UniRef50_UPI001939F8FF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7218.2438, "isoelectric_point": 7.9542543411254885 }, "id": "DFOKDJOJIG_779", "locus": "GCA013367855_00767" }, { "type": "cds", "sequence": "contig_1", "start": 432430, "stop": 432612, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF", "UniRef:UniRef90_UPI0015F0C8A9" ], "nt": "TTGATTCCTAGAAAAGTTTTGTTCGAAGCAAAATTTATTGATAATGAAAATGTTGATTTAAAAGAAGCATTCTTTAAATATAATTCAGGATATTCGAACTTTAAAAAAACTAAAGTTTATGCAAATTTAAAAAGCGGTGAAATCACAATTAAAAACTTTGAATTAGGTGCTGCAAAAGATTGA", "aa": "MIPRKVLFEAKFIDNENVDLKEAFFKYNSGYSNFKKTKVYANLKSGEITIKNFELGAAKD", "aa_hexdigest": "c99ae0f6042e4aa49d5068b8b0f8f5d8", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 432244, "stop": 432594, "observations": { "mutation": [ 432432 ], "stop": [ 432432 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "query_cov": 0.9, "subject_cov": 0.46551724137931033, "identity": 0.981, "score": 108.0, "evalue": 1.64e-29, "gene_start": 7, "gene_end": 60, "reference_start": 1, "reference_end": 54, "reference_sequence": "MFEAKFIDNENVDLKEAFFKYNSGYSNFKKTKVYANLKSGEITIKNFELGAAKDWKELPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK" }, "paralog": false, "identity": 0.9827586206896551, "score": 234.0, "evalue": 2.48e-81, "description": "Internal stop codon at 432432. Nonsense mutation around 432432.", "psc": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0015F0C8A9", "UniRef:UniRef50_UPI001939F8FF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6924.882300000001, "isoelectric_point": 9.260577201843262 }, "id": "DFOKDJOJIG_780", "locus": "GCA013367855_00768" }, { "type": "cds", "sequence": "contig_1", "start": 433251, "stop": 433676, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B6", "UniRef:UniRef90_UPI00158BFF58" ], "nt": "TTGCCAATATTGCCCTTATTAGTAACTTCTTGCAATAATAGAGAAACTGAATTAAAAAATGCCGAAACAATTATTGGCAATAAAGAAATAGTTTTTAATATGGTTGATATTTTAAATAGCGAAGAAGAATCAGTTTTAAATAACGACGCAACTATTTCTGGCCCATGGCGTAATGATTTTTTTAAAATTGTAATAACATCAAAAAAGAATTTTGATAAATATGAAAAAGATTATTGTGTTTTTGGAAAGATAGATTTTTATGCATATTCCATTTTGTTTTTAATTCCTAAAAAAGTATTATTAGAAACAGGATTTATTTCAAGCGAAGATTTTAATTTAGCAGACTTTTATAGTGAAAAACTTAATGTTGCGTTTAATGAACAGCCTAGAAAAATTTCAACAAATTTAAAAAATAAAAAAATATGA", "aa": "MPILPLLVTSCNNRETELKNAETIIGNKEIVFNMVDILNSEEESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSEKLNVAFNEQPRKISTNLKNKKI", "aa_hexdigest": "227615fa7fdab2041ec23ff44197a3c8", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 433078, "stop": 433724, "observations": { "deletion": [ 433142 ], "mutation": [ 433253, 433709 ], "stop": [ 433085, 433127, 433133, 433253, 433709 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158BFF58", "query_cov": 1.0, "subject_cov": 0.6558139534883721, "identity": 0.986, "score": 275.0, "evalue": 1.29e-92, "gene_start": 1, "gene_end": 141, "reference_start": 17, "reference_end": 157, "reference_sequence": "MKKNKWKLLTIFGISALPILPLFVTSCNNRETELKNAETIIGNKEIVFNMVDILNSEEESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSEKLNVAFNEQPRKISTNLKNKKIWIYKYMAPVHKLDDKNNKPELRFFYIKQLMLDCSLNKKKTWTWCSFVIYSTPKKRIWF" }, "paralog": false, "identity": 0.9674418604651163, "score": 406.0, "evalue": 1.61e-146, "description": "Internal stop codon at 433085, 433127, 433133, 433253, 433709. Frameshift due to deletion around 433142. Nonsense mutation around 433253, 433709.", "psc": { "uniref90_id": "UniRef90_UPI00158BFF58", "uniref50_id": "UniRef50_D1J8B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158BFF58", "UniRef:UniRef50_D1J8B6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B6" ], "product": "Lipoprotein" } }, "id": "DFOKDJOJIG_781", "locus": "GCA013367855_00769" }, { "type": "cds", "sequence": "contig_1", "start": 433793, "stop": 433963, "strand": "-", "frame": 3, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9", "UniRef:UniRef90_D1J8B7" ], "nt": "ATGCCTAATCCAATAAAAAAAGGAAAAACTGGTCATGCGATTGTTGCTTATGGAATGTATGACAATGGAAATAAACCTCTTTGCCATTATGGATGGGGAGGATATTCTCAAGTAATTGTTTCTTCAAGTCTTTCTGGCGAATTATTTTTATTGGGCATGAAACCAAACTGA", "aa": "MPNPIKKGKTGHAIVAYGMYDNGNKPLCHYGWGGYSQVIVSSSLSGELFLLGMKPN", "aa_hexdigest": "edcceee7535db6ec51328c675b966a84", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 433724, "stop": 433963, "observations": { "mutation": [ 433795 ], "stop": [ 433795 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8B7", "query_cov": 1.0, "subject_cov": 0.7088607594936709, "identity": 0.9640000000000001, "score": 120.0, "evalue": 8.86e-35, "gene_start": 1, "gene_end": 56, "reference_start": 1, "reference_end": 56, "reference_sequence": "MPNPIKKGKIGHAIVAYGMYDNGNKPLCHYGWGGYSQVIVSSSLSGKLFLLGMKPNGKVYKTRKHFIKKGIKYSGKDFK" }, "paralog": false, "identity": 0.9493670886075949, "score": 162.0, "evalue": 1.08e-53, "description": "Internal stop codon at 433795. Nonsense mutation around 433795.", "psc": { "uniref90_id": "UniRef90_D1J8B7", "uniref50_id": "UniRef50_A0A6A8Q0U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8B7", "UniRef:UniRef50_A0A6A8Q0U9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q0U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9" ], "product": "Peptidase C51 domain-containing protein" } }, "id": "DFOKDJOJIG_782", "locus": "GCA013367855_00770" }, { "type": "cds", "sequence": "contig_1", "start": 434371, "stop": 434550, "strand": "-", "frame": 1, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53", "UniRef:UniRef90_UPI0023AACE53" ], "nt": "ATGCAACAACAAAACAAAAAATATAATTTTGAATTCAAACTTGGTGCAAGCAATAAAATTATTGATTTGGCAGCAAATTTTATTTTGAAATATGGTCAAAAGACATTGCGCGAGGTTGCAAAAGTTTCTTTTAAAACAACAATAAAAGCATTAGAAAAAATAGATAATAAATTAGAATAA", "aa": "MQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLREVAKVSFKTTIKALEKIDNKLE", "aa_hexdigest": "0084eb09148121a8e6683e2b4ad655e1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 434371, "stop": 434679, "observations": { "mutation": [ 434553, 434664 ], "stop": [ 434553, 434664 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AACE53", "query_cov": 1.0, "subject_cov": 0.5784313725490197, "identity": 0.983, "score": 110.0, "evalue": 1.81e-30, "gene_start": 1, "gene_end": 59, "reference_start": 44, "reference_end": 102, "reference_sequence": "MQAISWLDFKKPQNEIIIETKGEEKSLAVAAASIISRKFLIDWMQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLHEVAKVSFKTTIKALEKIDNKLE" }, "paralog": false, "identity": 0.9607843137254902, "score": 185.0, "evalue": 2.83e-62, "description": "Internal stop codon at 434553, 434664. Nonsense mutation around 434553, 434664.", "psc": { "uniref90_id": "UniRef90_UPI0023AACE53", "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AACE53", "UniRef:UniRef50_UPI0023AACE53" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53" ] } }, "id": "DFOKDJOJIG_783", "locus": "GCA013367855_00771" }, { "type": "cds", "sequence": "contig_1", "start": 434662, "stop": 434946, "strand": "-", "frame": 1, "gene": null, "product": "ribonuclease HIII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1B727", "UniRef:UniRef90_UPI0034A1B727" ], "nt": "GTGATAACAAATTTTTTGAGGTTATTTCATGAAAAAATAATTGAAATTTTTAATCAAATTGAAAATTTAGTTTATTATCAAAAAACTTACCTTAGTCAAAGTGGTTATAATAAATTAATTGATGCAGGTATTAATAACAACGAAATAAAAACATTAATTCATAGTAATTCAATAAATATATTAACAAAAAAATTAAATCAGAACATTGATGTTTTTATTGATCAATATACAACTTCATTAGAAGTGTTACAAAAGCATTTCAAAAAACTGCAAGCAATTTCTTGA", "aa": "MITNFLRLFHEKIIEIFNQIENLVYYQKTYLSQSGYNKLIDAGINNNEIKTLIHSNSINILTKKLNQNIDVFIDQYTTSLEVLQKHFKKLQAIS", "aa_hexdigest": "db52716a1bee157fa1fa448ae6665ff3", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 434398, "stop": 435006, "observations": { "mutation": [ 434553, 434664 ], "stop": [ 434553, 434664 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A1B727", "query_cov": 1.0, "subject_cov": 0.46534653465346537, "identity": 0.9790000000000001, "score": 179.0, "evalue": 1.89e-55, "gene_start": 1, "gene_end": 94, "reference_start": 21, "reference_end": 114, "reference_sequence": "VKKYKNSSVKNIPLEFFKFKVITNFLRLFHEKIIEIFNQIENLVYYQKTYLSQSGYNKLIDAGINNNEIKTLIHSNSINILTKKLNQNIDVFIDQYTTSLEMLQKHFKKLQAISWLDFKKPQNEIIIETKGEEKSLAVAAASIISRKFLIDWMQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLREVAKVSFKTTIKA" }, "paralog": false, "identity": 0.9851485148514851, "score": 383.0, "evalue": 1.62e-138, "description": "Internal stop codon at 434553, 434664. Nonsense mutation around 434553, 434664.", "psc": { "uniref90_id": "UniRef90_UPI0034A1B727", "product": "ribonuclease HIII", "uniref50_id": "UniRef50_UPI0034A1B727", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A1B727", "UniRef:UniRef50_UPI0034A1B727" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A1B727", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1B727" ], "product": "ribonuclease HIII" } }, "id": "DFOKDJOJIG_784", "locus": "GCA013367855_00772" }, { "type": "cds", "sequence": "contig_1", "start": 435009, "stop": 435188, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_158532111.1", "SO:0001217", "UniParc:UPI001313F21A", "UniRef:UniRef100_UPI0027336384", "UniRef:UniRef50_D1J8B5", "UniRef:UniRef90_D1J8B5" ], "nt": "ATGCCTGGTTTATATATTAATATTCACAAATTAACAATTCATATGAATTCAGATCCTAAAACAATTGAAATGGATTTTAGCTTAAAATATTCAAAATCTAAAGAACATCAACAAAAGATTGAAGGTTTTGAAGATGACTATATTTTTGAATATAAATATATATTCAAAGAAAAAAAGTAA", "aa": "MPGLYINIHKLTIHMNSDPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK", "aa_hexdigest": "6195c649747e61396612f08aa58aa70f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001313F21A", "ncbi_nrp_id": "WP_158532111.1", "uniref100_id": "UniRef100_UPI0027336384", "db_xrefs": [ "SO:0001217", "UniParc:UPI001313F21A", "RefSeq:WP_158532111.1", "UniRef:UniRef100_UPI0027336384" ] }, "ips": { "uniref100_id": "UniRef100_UPI0027336384", "uniref90_id": "UniRef90_D1J8B5" }, "psc": { "uniref90_id": "UniRef90_D1J8B5", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J8B5" }, "pscc": { "uniref50_id": "UniRef50_D1J8B5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B5" ], "product": "Lipoprotein" }, "genes": [], "id": "DFOKDJOJIG_785", "locus": "GCA013367855_00773" }, { "type": "cds", "sequence": "contig_1", "start": 435866, "stop": 435988, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCCCGGTGCATAAATTAGATGATAAGAATAGTGAACCCAGGTTGCGCTTTTACATTAAACAATTAACGTTAGATTGCAATCTAAACAAAAAAAGACTTGAACTTGATGTTCATTTATGA", "aa": "MAPVHKLDDKNSEPRLRFYIKQLTLDCNLNKKRLELDVHL", "aa_hexdigest": "d6aae8907188a7a9841541535ed99b59", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4805.582899999999, "isoelectric_point": 8.978850364685059 }, "id": "DFOKDJOJIG_786", "locus": "GCA013367855_00774" }, { "type": "cds", "sequence": "contig_1", "start": 436001, "stop": 436426, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B6", "UniRef:UniRef90_UPI00158BFF58" ], "nt": "TTGCCAATATTGCCCTTATTTGTAACCTCTTGCAATAATAGAGAAACTGAATTAAAAAATGCCGAAACAATTATTGGCAATAAAGAAATAGTTTTTAATATGGTTGATATTTTAAATAGCGAAGAAGAATCAGTTTTAAATAACGACGCAACTATTTCTGGCCCATGGCGTAATGATTTTTTTAAAATTGTAATAACATCAAAAAAGAATTTTGATAAATATGAAAAAGATTATTGTGTTTTTGGAAAGATAGATTTTTATGCATATTCCATTTTGTTTTTAATTCCTAAAAAAGTATTATTAGAAACAGGATTTATTTCAAGCGAAGATTTTAATTTAGCAGACTTTTATAGTAAAAACCTTAATTTCGTGTTTAATGAACAACCTAGAAAAATTTCAACAAATTTAAAAAATAAAAAAATATGA", "aa": "MPILPLFVTSCNNRETELKNAETIIGNKEIVFNMVDILNSEEESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSKNLNFVFNEQPRKISTNLKNKKI", "aa_hexdigest": "f8ed3850db5ed24a03fc2e56871cc953", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 435831, "stop": 436474, "observations": { "deletion": [ 435895 ], "mutation": [ 436003, 436459 ], "stop": [ 435838, 435880, 435886, 436003, 436459 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158BFF58", "query_cov": 1.0, "subject_cov": 0.6558139534883721, "identity": 0.965, "score": 271.0, "evalue": 8.64e-91, "gene_start": 1, "gene_end": 141, "reference_start": 17, "reference_end": 157, "reference_sequence": "MKKNKWKLLTIFGISALPILPLFVTSCNNRETELKNAETIIGNKEIVFNMVDILNSEEESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSEKLNVAFNEQPRKISTNLKNKKIWIYKYMAPVHKLDDKNNKPELRFFYIKQLMLDCSLNKKKTWTWCSFVIYSTPKKRIWF" }, "paralog": false, "identity": 0.9209302325581395, "score": 385.0, "evalue": 2.64e-138, "description": "Internal stop codon at 435838, 435880, 435886, 436003, 436459. Frameshift due to deletion around 435895. Nonsense mutation around 436003, 436459.", "psc": { "uniref90_id": "UniRef90_UPI00158BFF58", "uniref50_id": "UniRef50_D1J8B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158BFF58", "UniRef:UniRef50_D1J8B6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B6" ], "product": "Lipoprotein" } }, "id": "DFOKDJOJIG_787", "locus": "GCA013367855_00775" }, { "type": "cds", "sequence": "contig_1", "start": 436554, "stop": 436712, "strand": "-", "frame": 2, "gene": null, "product": "putative cysteine peptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9", "UniRef:UniRef90_UPI0012AACF5E" ], "nt": "ATGCCTAATCCAATAAAAAAAGGAAAAATTGGTCATGCAATTGTTGCTTATGGAATGTATGACAATGGAAATAAACCTCTTTGCCATTATGGATGGGGAGGATATTCTCAAGTTATTGTTTCTTCGAGTCTTTCTGGCGAATTATTTTATTGGGCATGA", "aa": "MPNPIKKGKIGHAIVAYGMYDNGNKPLCHYGWGGYSQVIVSSSLSGELFYWA", "aa_hexdigest": "dbfcde80fe72685e479e383ebd489fc7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 436461, "stop": 436712, "observations": { "insertion": [ 436547 ], "deletion": [ 436565 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0012AACF5E", "query_cov": 0.9423076923076923, "subject_cov": 0.5903614457831325, "identity": 0.98, "score": 108.0, "evalue": 9.07e-30, "gene_start": 1, "gene_end": 49, "reference_start": 1, "reference_end": 49, "reference_sequence": "MPNPIKKGKIGHAIVAYGMYDNGNKPLCHYGWGGYSQVIVSSSLSGQLFLLGMKPMERFIKQGNILSKRELNIRERILNNEKK" }, "paralog": false, "identity": 0.9879518072289156, "score": 161.0, "evalue": 3.04e-53, "description": "Frameshift due to insertion around 436547. Frameshift due to deletion around 436565.", "psc": { "uniref90_id": "UniRef90_UPI0012AACF5E", "product": "putative cysteine peptidase", "uniref50_id": "UniRef50_A0A6A8Q0U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0012AACF5E", "UniRef:UniRef50_A0A6A8Q0U9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q0U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9" ], "product": "Peptidase C51 domain-containing protein" } }, "id": "DFOKDJOJIG_788", "locus": "GCA013367855_00776" }, { "type": "cds", "sequence": "contig_1", "start": 437120, "stop": 437299, "strand": "-", "frame": 3, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53", "UniRef:UniRef90_UPI0023AACE53" ], "nt": "ATGCAACAACAAAATAAAAAATATAATTTTGAATTCAAACTTGGTGCAAGCAATAAAATTATTGATTTGGCAGCAAATTTTATTTTGAAATATGGTCAAAAGACATTGCACGAAGTTGCAAAAGTTTCTTTTAAAACAACAATAAAAGCATTAGAAAAAATAGATAATAAATTAGAATAA", "aa": "MQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLHEVAKVSFKTTIKALEKIDNKLE", "aa_hexdigest": "46b5217c9ab687d05b9b410f52e26899", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 437120, "stop": 437428, "observations": { "mutation": [ 437302, 437413 ], "stop": [ 437302, 437413 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AACE53", "query_cov": 1.0, "subject_cov": 0.5784313725490197, "identity": 1.0, "score": 114.0, "evalue": 5.41e-32, "gene_start": 1, "gene_end": 59, "reference_start": 44, "reference_end": 102, "reference_sequence": "MQAISWLDFKKPQNEIIIETKGEEKSLAVAAASIISRKFLIDWMQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLHEVAKVSFKTTIKALEKIDNKLE" }, "paralog": false, "identity": 0.9705882352941176, "score": 188.0, "evalue": 1.74e-63, "description": "Internal stop codon at 437302, 437413. Nonsense mutation around 437302, 437413.", "psc": { "uniref90_id": "UniRef90_UPI0023AACE53", "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AACE53", "UniRef:UniRef50_UPI0023AACE53" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53" ] } }, "id": "DFOKDJOJIG_789", "locus": "GCA013367855_00777" }, { "type": "cds", "sequence": "contig_1", "start": 437411, "stop": 437818, "strand": "-", "frame": 3, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51E35B", "UniRef:UniRef90_UPI001F51E35B" ], "nt": "TTGAATAAAAATTTAGATGAATTAATTGGCGTTGATGAAACGGGAGTAGGTGATTATTTCAGCCCCGTAATTTCAGTTGCCTGTTATATACCAAAAGATAACATTGAAAAACTAAAACAAATTGGTGTTAAAGACAGCAAAAAACTTTCTGATAAAAGAATAATTGAAATTTTTAATCAAATTGAAAATTTAGTTTATTATCAAAAAACTTGCCTTAGTCAAAGTGGTTATAATAAATTAATTGATGCAGGTATTAATAACAACGAAATAAAAACATTAATTCATAGTAATTCAATAAATATATTAACAAAAAAATTAAATCAGAACATTGATGTTTTTATTGATCAATATACAACTTCATTAGAAATGTTGCAAAAGCATTTCAAAAAATTGCAAGCAATTTCTTGA", "aa": "MNKNLDELIGVDETGVGDYFSPVISVACYIPKDNIEKLKQIGVKDSKKLSDKRIIEIFNQIENLVYYQKTCLSQSGYNKLIDAGINNNEIKTLIHSNSINILTKKLNQNIDVFIDQYTTSLEMLQKHFKKLQAIS", "aa_hexdigest": "8ab1c7743e6c47e26e58a7dfe7cf5fda", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI001F51E35B", "query_cov": 0.9259259259259259, "subject_cov": 0.9057971014492754, "identity": 1.0, "score": 246.0, "evalue": 2e-82, "valid": true, "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI001F51E35B" }, "pscc": { "uniref50_id": "UniRef50_UPI001F51E35B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51E35B" ] }, "genes": [], "id": "DFOKDJOJIG_790", "locus": "GCA013367855_00778" }, { "type": "cds", "sequence": "contig_1", "start": 437811, "stop": 438119, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAGATATTACAACATTGACATTGATGATACTGTTGCAATGGGAGATTCATATAATGACTTAAGCATGTATAATGTTGCTAATGTTTGTGTATCACCTGCAAATGCTGAACCCTTAATTAAAAAGATGTCAACAGTTGTTATGAAGCAAACAAACAAAGAAGGCGCAGTTGGATACTTTATTGATCAATTCCTAAAAAACCCAGAAAAATACATTAAATTAGGTAAAAAACAAAAGGCCGAACAAAAAAATAATATTAAAACTGTAGGTGCAGATAACTTTTTTAGTAAAGAAGAAGAAATTGAATAA", "aa": "MRYYNIDIDDTVAMGDSYNDLSMYNVANVCVSPANAEPLIKKMSTVVMKQTNKEGAVGYFIDQFLKNPEKYIKLGKKQKAEQKNNIKTVGADNFFSKEEEIE", "aa_hexdigest": "c8525aacea873a6f3ab319125237b291", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 60, "aa_cov": 0.5882352941176471, "hmm_cov": 0.9833333333333333, "evalue": 3.633647298292107e-13, "score": 45.427085876464844, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 11611.095899999991, "isoelectric_point": 5.506129646301268 }, "id": "DFOKDJOJIG_791", "locus": "GCA013367855_00779" }, { "type": "cds", "sequence": "contig_1", "start": 438198, "stop": 438593, "strand": "-", "frame": 2, "gene": "cof", "product": "Putative phosphatase in upp 3'region", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "KEGG:K21064", "SO:0001217", "UniRef:UniRef50_P43051", "UniRef:UniRef90_P43051" ], "nt": "TTGCCTGTGTATGAAAAACTTGGACTTAATGCAATTGTAGGTAATTACAATGGTGCTCACATCCACAACCCTGCTGACCCATTCTTTATACCTGCTATTACATATTTGGACTTAAATGAAGTTCTTTATATTTTAGGCGATGAAAAAGTAAAAAAAGAAATTACTAATTATGCAATAGAAGGCCCTGACTGGGTTCAATTAATGCATAGAGATCCTAACCTTGAAAGAGTGTTTGGATTTAACCAAGCAACAAAATTTAGAGAATGTATTAACCTAGAAAAAATTCCACTAAAACCAACCGGAATAGTATTCGATGTTAAACCTGATACTGATGTCTTGGAATTATTGACATATTTAAAAAGACGTTATGGAGATTTAGGAGAATTTTCTTCTTGA", "aa": "MPVYEKLGLNAIVGNYNGAHIHNPADPFFIPAITYLDLNEVLYILGDEKVKKEITNYAIEGPDWVQLMHRDPNLERVFGFNQATKFRECINLEKIPLKPTGIVFDVKPDTDVLELLTYLKRRYGDLGEFSS", "aa_hexdigest": "c77529f2e3482b857b9e30296c47109d", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [ "cof" ], "truncated": "both", "pseudogene": { "start": 437895, "stop": 438773, "observations": { "mutation": [ 438200, 438605 ], "stop": [ 438200, 438605 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43051", "query_cov": 1.0, "subject_cov": 0.409375, "identity": 0.992, "score": 275.0, "evalue": 2.73e-91, "gene_start": 1, "gene_end": 131, "reference_start": 61, "reference_end": 191, "reference_sequence": "MAYNKESKQRRFLFAIDLDGTLLADSANGTVHPKTEEAIKKAVAQGHIVSIITGRPWRSTLPVYEKLGLNAIVGNYNGAHIHNPADPFFIPAITYLDLNEVLYILGDEKVKKEITNYAIEGPDWVQLMHRDPNLERVFGFNQATKFRECINLEKIPLKPTGIVFDVKPDTDVLELLTYLKRRYGDLGEFSSWSKGEGLSPVFDITSIGIDKGKVISLIMRYYNIDIDDTVAMGDSYNDLSMYNVANVCVSPANAEPLIKKMSTVVMKQTNKEGAVGYFIDQFLKNPEKYIKLGKKQKAEQKNNIKTVGADNFFSKEEEIE" }, "paralog": false, "identity": 0.9931506849315068, "score": 580.0, "evalue": 7.4e-214, "description": "Internal stop codon at 438200, 438605. Nonsense mutation around 438200, 438605.", "psc": { "uniref90_id": "UniRef90_P43051", "gene": "Cof", "product": "Putative phosphatase in upp 3'region", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P43051", "cog_id": "COG0561", "cog_category": "HR", "kegg_orthology_id": "K21064", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43051", "EC:3.1.3.-", "UniRef:UniRef50_P43051", "COG:COG0561", "COG:HR", "KEGG:K21064", "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P43051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43051" ], "product": "Putative phosphatase in upp 3'region" } }, "id": "DFOKDJOJIG_792", "locus": "GCA013367855_00780" }, { "type": "cds", "sequence": "contig_1", "start": 438603, "stop": 438773, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGGCGTACAACAAAGAATCAAAACAAAGAAGATTTTTATTTGCTATTGATCTAGATGGAACACTATTAGCAGATTCAGCCAATGGCACAGTTCATCCAAAAACAGAAGAAGCAATTAAAAAAGCTGTTGCCCAAGGACACATTGTTTCAATAATTACAGGAAGACCTTGA", "aa": "MAYNKESKQRRFLFAIDLDGTLLADSANGTVHPKTEEAIKKAVAQGHIVSIITGRP", "aa_hexdigest": "b4070446689c7714536aa9e25714399e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 43, "aa_cov": 0.7678571428571429, "hmm_cov": 0.9534883720930233, "evalue": 8.360397546122954e-12, "score": 40.96463394165039, "start": 14, "stop": 56 } ], "seq_stats": { "molecular_weight": 6108.936900000001, "isoelectric_point": 9.30106334686279 }, "id": "DFOKDJOJIG_793", "locus": "GCA013367855_00781" }, { "type": "cds", "sequence": "contig_1", "start": 438957, "stop": 439586, "strand": "-", "frame": 2, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0009116", "GO:0044206", "RefSeq:WP_175972290.1", "SO:0001217", "UniParc:UPI0000137D2B", "UniRef:UniRef100_P43049", "UniRef:UniRef50_Q98QP6", "UniRef:UniRef90_P43049" ], "nt": "ATGGTAAAAATTTTTCAGCATCCTTTAATTAACGCTAAATTAACAACGATGCGTTCAAAAGAAACATCTTACAAAGATTTTAGAGATAATTTGAACGAGATTGCTTCGTTGATGGTATATGAAACATTGAGGGACTATCAAACTAAGAAAATATCAATAACAACACCAATGAATGTTAAATATGAAGGCGAAACTCTTGATAGAGAAATTGTAATTATTCCTATTTTACGTGCGGGGCTTGGAATGTTGAATGGTATTATGAATTTGGTTCCCCAAGCTAGAGTTGGTCATATTGGAATGTATAGAAACGAAGAAACAAACGAAGTTGTTGAATATTTCTTTAAAATTCCAGAAGTACCACATGATAGCTATATTATTATTGTTGACCCTATGCTTGCAACCGGCTCATCTGCATGCGATGCAATAGCAAAATTGGATAAGTTAGGCTTTAACAATATTAAATTAGTATGTTTAGTAGGCGTTCAACAAGGTATTGATAAAGTAACAAAACAATTTCCTAATGTTGATATATATTTAGCTTCAAAAGATGAAAAATTGAATGAACACAATTATATATTGCCTGGTTTGGGAGATGCTGGTGACCGTATATTTGGAACAAAAATAAAATAA", "aa": "MVKIFQHPLINAKLTTMRSKETSYKDFRDNLNEIASLMVYETLRDYQTKKISITTPMNVKYEGETLDREIVIIPILRAGLGMLNGIMNLVPQARVGHIGMYRNEETNEVVEYFFKIPEVPHDSYIIIVDPMLATGSSACDAIAKLDKLGFNNIKLVCLVGVQQGIDKVTKQFPNVDIYLASKDEKLNEHNYILPGLGDAGDRIFGTKIK", "aa_hexdigest": "93cccf63e72644edac1e7072fd950eda", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137D2B", "ncbi_nrp_id": "WP_175972290.1", "uniref100_id": "UniRef100_P43049", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137D2B", "RefSeq:WP_175972290.1", "UniRef:UniRef100_P43049" ] }, "ips": { "uniref100_id": "UniRef100_P43049", "uniref90_id": "UniRef90_P43049" }, "psc": { "uniref90_id": "UniRef90_P43049", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_Q98QP6", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0009116", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP6" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "DFOKDJOJIG_794", "locus": "GCA013367855_00782" }, { "type": "cds", "sequence": "contig_1", "start": 439614, "stop": 440276, "strand": "-", "frame": 2, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "db_xrefs": [ "COG:COG0274", "COG:F", "EC:4.1.2.4", "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052", "KEGG:K01619", "RefSeq:WP_020002489.1", "SO:0001217", "UniParc:UPI000378BCA6", "UniRef:UniRef100_A0A2K9YTE4", "UniRef:UniRef50_Q98QP7", "UniRef:UniRef90_P43048" ], "nt": "ATGACAGAATTAAACAAATATATTGACCACACAAATTTATCACCTTCTGCAACAAGCAAAGACATTGATAAATTAATTCAAGAAGCAATTAAATATGATTTTAAATCAGTATGTATTGCACCATCATATGTTAAGTATGCTAAAGAAGCATTAAAAAACAGTGATGTGCTTGTTTGCACAGTTATTGGTTTTCCACTAGGCTATAATGCAACAAGTGTTAAAGTATATGAAACAAAAATTGCTGTTGAACATGGTGCTGACGAAATTGATATGGTAATCAATGTTGGAAGATTTAAAGATGGACAATATGAATACGTATTAAACGAAATCAAAGCAATAAAAGAAGCATGCAATGGCAAAACTTTAAAAGTTATAGTTGAAACAGCATTGTTAACAAAAGCTGAATTAATAAAAATTACTGAATTAGTAATGCAATCTGGCGCCGATTTTATTAAGACATCAACCGGATTTTCATATAGAGGAGCTTCATTTGAAGACATTCAAACTATGAAGGAAACCTGTGGTGACAAGCTTTTAATTAAAGCATCAGGTGGTATTAAGAATCTAGCGGATGCCCAAGAAATGATTAGATTAGGTGCAAACCGTTTGGGTATGTCTAAATCAGTTCCAGTTATGGAAGAATTATTTAAATTAGAAAAATAA", "aa": "MTELNKYIDHTNLSPSATSKDIDKLIQEAIKYDFKSVCIAPSYVKYAKEALKNSDVLVCTVIGFPLGYNATSVKVYETKIAVEHGADEIDMVINVGRFKDGQYEYVLNEIKAIKEACNGKTLKVIVETALLTKAELIKITELVMQSGADFIKTSTGFSYRGASFEDIQTMKETCGDKLLIKASGGIKNLADAQEMIRLGANRLGMSKSVPVMEELFKLEK", "aa_hexdigest": "58846acd5aac329a1c0a28ee84221e78", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000378BCA6", "ncbi_nrp_id": "WP_020002489.1", "uniref100_id": "UniRef100_A0A2K9YTE4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000378BCA6", "RefSeq:WP_020002489.1", "UniRef:UniRef100_A0A2K9YTE4" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTE4", "uniref90_id": "UniRef90_P43048" }, "psc": { "uniref90_id": "UniRef90_P43048", "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_Q98QP7", "cog_id": "COG0274", "cog_category": "F", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP7" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [ "deoC" ], "id": "DFOKDJOJIG_795", "locus": "GCA013367855_00783" }, { "type": "cds", "sequence": "contig_1", "start": 440303, "stop": 440527, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "ATGAAATTGGGAGCAGGTAGATCTAAAAAAGAAGATTCTATCGACTATGAAGCAGGAATTTATTTGAATAAGAGTTCAAATGAATATGTAAATAAAGGAGATGTATTATTTACAATGTATTCGTCAAAACCTATAAATCCAGAACTTCAAAAAGAATTGTTGTCTGCAATAGAATTTAGCGAAAGCAAGCATGACATTCAAACGGTTTTTGCTAAATTAATGTAA", "aa": "MKLGAGRSKKEDSIDYEAGIYLNKSSNEYVNKGDVLFTMYSSKPINPELQKELLSAIEFSESKHDIQTVFAKLM", "aa_hexdigest": "d2f2bac891b8cdf8503fc70cdea71e81", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 60, "aa_cov": 0.7972972972972973, "hmm_cov": 1.0, "evalue": 2.9387041769320834e-21, "score": 70.6019515991211, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 8377.444499999994, "isoelectric_point": 5.74621753692627 }, "id": "DFOKDJOJIG_796", "locus": "GCA013367855_00784" }, { "type": "cds", "sequence": "contig_1", "start": 440666, "stop": 441277, "strand": "-", "frame": 3, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A386RHI4" ], "nt": "ATGTCAGGAAGAGGACTTGGACATACAGGAGGAACACTTGATAAATTAGAATCAATTGACGGTTATAATTTTTCAGTGACTGACCAACAATTTAGAGACTTAGTTGAAAAGCATGGTATTGCAATAGTTGGACAAAATCAAAAACTTGTGCCTGCAGATAAAAAAATCTATGCATTACGTGATGTAACTGGCACCGTTCAATCAATTCCATTGATCGCCTCATCAATTATGTCTAAAAAACTTGCAACCGGTTCAAACTGCATTTTATTGGATGTTAAATGTGGCAATGGCGCATTTATGAAAGATATTAATGAAGCAAAAAAACTTGGAAAATTGATGATTGAAATTGGCAAGAAATTAAATAGAAAGATTGCCGTTGAAATAACCAATATGCAACAACCTTTAGGAAAAACTATTGGAAATAAAATTGAAGTTTTAGAAGCAATAGATACATTAAATGGACACGGACCTAAAGATTTTACAGAAATTATTTACTCATCAGGTTCAACGTTATTAGTTTTAGCCCAAAAAGCTAAAGATGAAGTTGAAGCAAGAAAAATGATTGATGAAGTAATCAATAATAAAAAAGCATATAACAAATTTTTGGAATGA", "aa": "MSGRGLGHTGGTLDKLESIDGYNFSVTDQQFRDLVEKHGIAIVGQNQKLVPADKKIYALRDVTGTVQSIPLIASSIMSKKLATGSNCILLDVKCGNGAFMKDINEAKKLGKLMIEIGKKLNRKIAVEITNMQQPLGKTIGNKIEVLEAIDTLNGHGPKDFTEIIYSSGSTLLVLAQKAKDEVEARKMIDEVINNKKAYNKFLE", "aa_hexdigest": "c73401cc8d28be398ff1038470d4c93b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A386RHI4", "query_cov": 0.8275862068965517, "subject_cov": 0.8527918781725888, "identity": 0.53, "score": 182.0, "evalue": 3.36e-55, "valid": false, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0A386RHI4", "go_ids": [ "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0A386RHI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A386RHI4" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "DFOKDJOJIG_797", "locus": "GCA013367855_00785" }, { "type": "cds", "sequence": "contig_1", "start": 441389, "stop": 441601, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02885.22" ], "nt": "ATGAGAATTATTGATATTATTAACAAAAAAGTAGAAAATCAAACTCTTACCAAGGAAGAAATTGATTTTTTCATTAAAGAATATGTAGCAGAAAATGTTCCTGACTACCAAGCTGCTGCTTTATTAATGGCAATTAGATTAAATGGTTTAAGCGATGCTGAAACAGCGCAATTAACCGACGCTATGATGCATTCAGGCGAAGTACTTGATTGA", "aa": "MRIIDIINKKVENQTLTKEEIDFFIKEYVAENVPDYQAAALLMAIRLNGLSDAETAQLTDAMMHSGEVLD", "aa_hexdigest": "36b15e58d0f699d7be26cfe5892d5407", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02885.22", "name": "Glycos_trans_3N", "length": 62, "aa_cov": 0.8857142857142857, "hmm_cov": 1.0, "evalue": 2.978854829229589e-25, "score": 83.47432708740234, "start": 5, "stop": 66 } ], "seq_stats": { "molecular_weight": 7899.955200000003, "isoelectric_point": 4.414707374572754 }, "id": "DFOKDJOJIG_798", "locus": "GCA013367855_00786" }, { "type": "cds", "sequence": "contig_1", "start": 441618, "stop": 442334, "strand": "-", "frame": 2, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0006139", "KEGG:K03784", "RefSeq:WP_020002487.1", "SO:0001217", "UniParc:UPI00035C52D1", "UniRef:UniRef100_A0A6A8Q0U0", "UniRef:UniRef50_A0A449A549", "UniRef:UniRef90_D1J8C3" ], "nt": "ATGACACCTCATATAAATGCAAAAGATGATGATTTTGCAAAATTAGTTTTAATGCCAGGTGATCCTTTAAGAGCAAAGTATATTGCTGAACACTATTTAGAAAACGCTAAGTTAGTGTCAACAGTTAGAAATGTTTTAATGTATACTGGCTTTTATAAAGGAAGAAAAATTTCTATTTGTGCTTCAGGCATGGGCGTTCCTTCAATTGGAATTTATTCATATGAATTATTTAGCCAATATGGAGTTGAAGCAATAATTAGAATTGGATCAGGTGGCTCATTCAAGGCTGATTTAAAAAATTATTCATTAGTTTTGGCAACTTCAGCATATAGTGATAGCACTTCATTCCGTAAAAATTTATTAGTCAATGATGAAAGTAATGTTGCGCTTCCAAATAGCGAATTAAATGATTTAATTAAAAAACATGCTAAGGAATTGAATTTAAAACTTGAAGAAGGAAGAATTTTATCGGAAGATGCCTTTTATTCAGTATTTTCTGTTGAAGATAGAGTTAAAAATTCAGGCGGAGCAGTTGCTGTTGAAATGGAAGCATATGGGTTATTTACAGTAGCAGAAAAATTGCACAAAAAAGCTGCAACTATTTTAACTATTTCTGATAATTTAATAACACATGAATATACAACCGCTGAAGAAAGACAAAATTCATTCAATCAAATGATGGAATTAGCATTATCAATATCAGAAGACTTTGAATAA", "aa": "MTPHINAKDDDFAKLVLMPGDPLRAKYIAEHYLENAKLVSTVRNVLMYTGFYKGRKISICASGMGVPSIGIYSYELFSQYGVEAIIRIGSGGSFKADLKNYSLVLATSAYSDSTSFRKNLLVNDESNVALPNSELNDLIKKHAKELNLKLEEGRILSEDAFYSVFSVEDRVKNSGGAVAVEMEAYGLFTVAEKLHKKAATILTISDNLITHEYTTAEERQNSFNQMMELALSISEDFE", "aa_hexdigest": "01e48862952be26218e13d3f83512d35", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00035C52D1", "ncbi_nrp_id": "WP_020002487.1", "uniref100_id": "UniRef100_A0A6A8Q0U0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00035C52D1", "RefSeq:WP_020002487.1", "UniRef:UniRef100_A0A6A8Q0U0" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8Q0U0", "uniref90_id": "UniRef90_D1J8C3" }, "psc": { "uniref90_id": "UniRef90_D1J8C3", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_A0A449A549", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0006139" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A549", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A549" ], "product": "Uridine phosphorylase" }, "genes": [ "deoD" ], "id": "DFOKDJOJIG_799", "locus": "GCA013367855_00787" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 442475, "stop": 442765, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 206.8, "evalue": 3.4e-22, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "TTACATGGCCAACTGCTACTACAAAGTAGAGGAAAATCCGCTCTAGCACTCGCTGTGATGCGAGTAGTGTTCATGCTAGACCCAATAAGTCTAGGCAACAATTTAGTTGACGACTAGTGCAACAGAGACGAGCTTATTTAGTTAAGATGCAACGATGTAAACTCCATGAGCTAGAAACCCAAATTTTGGTAGGGGAACTGGGAACAAGGAACCGAACAAAGAACCCGGAAGCCTATTGGCTTAGATAAATAGTTGGCGCTATTAAAATAGTACAGAAAGCGGTTTATAGTG", "id": "DFOKDJOJIG_41", "locus": "GCA013367855_00788" }, { "type": "cds", "sequence": "contig_1", "start": 442832, "stop": 443386, "strand": "+", "frame": 2, "gene": "recO", "product": "DNA repair protein RecO", "db_xrefs": [ "COG:COG1381", "COG:L", "GO:0003677", "GO:0006281", "GO:0006310", "KEGG:K03584", "SO:0001217", "UniRef:UniRef50_D1J8C4", "UniRef:UniRef90_D1J8C4" ], "nt": "ATGGAATTTGAGCTAAGTGGAATTGTAATTAATAAACAAAATGTTAATGAATTTGATGAAATAATATCATTACTAACAGATAAAGGCATTCAAAGAATTTTTTGCCCCGGGGTAAATAAAACTAATTCAAAAAATGGCCCCTCAATTTTAATTTTAAACTTAGTTAATTGTGAAGTCATTAAATCAGAAACAAGAAATTTATTACCTAGACTAAAAACTGCTTCGTTAATAAAAAATTTCCCCACAGATTATAGACTTTTAAATATTGCAAATGATTTAAAAACCTTCTTTAATTGTCTTGACAATTGCAATAATATTAATCTTATTAAAGAATATAAACATTGCTTAGATTTTATATTGTCAAAATCTGATAAAGTTGAAGCATATTTATTGTTTTTAGTTGTTTCTAGTTTAGGATTTAAGCCTAACTTAAATTCTTGTATTGAATGTAACAATGTTGAAAATATTGTAGATTTTAAACTATACAAGGGGGGATTTTTGTGTTCGATGCATTCAAACATTGTAAATGCTGATGTATCGTTATTGAGGTCTTGA", "aa": "MEFELSGIVINKQNVNEFDEIISLLTDKGIQRIFCPGVNKTNSKNGPSILILNLVNCEVIKSETRNLLPRLKTASLIKNFPTDYRLLNIANDLKTFFNCLDNCNNINLIKEYKHCLDFILSKSDKVEAYLLFLVVSSLGFKPNLNSCIECNNVENIVDFKLYKGGFLCSMHSNIVNADVSLLRS", "aa_hexdigest": "b2581d1461886c2142d9f5c53882a5a5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8C4", "query_cov": 1.0, "subject_cov": 0.8214285714285714, "identity": 0.995, "score": 367.0, "evalue": 3.92e-128, "valid": true, "gene": "recO", "product": "DNA repair protein RecO", "uniref50_id": "UniRef50_D1J8C4", "cog_id": "COG1381", "cog_category": "L", "kegg_orthology_id": "K03584", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C4" ], "product": "DNA replication/recombination mediator RecO N-terminal domain-containing protein" }, "genes": [ "recO" ], "id": "DFOKDJOJIG_800", "locus": "GCA013367855_00789" }, { "type": "cds", "sequence": "contig_1", "start": 443490, "stop": 443804, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5", "UniRef:UniRef90_D1J8C5" ], "nt": "GTGAATAAAAATGAACATATTTTTAGTCAATATATTGAAGATAAACAAGACAAAGAAACATTGGCTAAAATTTCTGATGATTTTAAAAATCTATTTGAATATTTAGAAAATAATTTTAAGCAATACTTACTATTTTATAATGAAGCATTTAATTTAATTATTAATTTACATAAAAAGAATATATCAAAGGAAGATTTAATAAAATTAAATGATTTTGAAGACATGTTACGTTCTAATAAAACAAATAACATTGATTTTAAAACATTGCAACTTAAGTTTAATGAGATAAAAAAAATATCTATTAACGATAAATAA", "aa": "MNKNEHIFSQYIEDKQDKETLAKISDDFKNLFEYLENNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEDMLRSNKTNNIDFKTLQLKFNEIKKISINDK", "aa_hexdigest": "3156cc41174a5dcf127f084558b90ad4", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 443490, "stop": 444104, "observations": { "mutation": [ 443864 ], "stop": [ 443864 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8C5", "query_cov": 1.0, "subject_cov": 0.4928909952606635, "identity": 0.9420000000000001, "score": 186.0, "evalue": 4.91e-58, "gene_start": 1, "gene_end": 104, "reference_start": 108, "reference_end": 211, "reference_sequence": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPNYGIFRNLRDYFEKRVAQYDFKKKIVDKNEHIFSQYIEDKQDKETLAKISDDFKNLFEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEGMLRSNKTNNIDFKTLQRKFDEIKKISINDK" }, "paralog": false, "identity": 0.9558823529411765, "score": 388.0, "evalue": 4.87e-140, "description": "Internal stop codon at 443864. Nonsense mutation around 443864.", "psc": { "uniref90_id": "UniRef90_D1J8C5", "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8C5", "UniRef:UniRef50_D1J8C5" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12644.232599999978, "isoelectric_point": 6.113965797424315 }, "id": "DFOKDJOJIG_801", "locus": "GCA013367855_00790" }, { "type": "cds", "sequence": "contig_1", "start": 443862, "stop": 444125, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5", "UniRef:UniRef90_D1J8C5" ], "nt": "ATGAAGTTATCAAAAAATCAAGAAAAAGTTCAAAATAAAAAACCGCATAGATTTTTAACTAGAAGATCAAAAATAGGCATTATTTCAGGAATCAGTGCAGTTATTGGATTGCCTTTTTTACTAGGTATTACTTATGTTCTTGTTAATAATCATAAAAAAACTAATTCAAACAGTCAATTAATAATAATTCGTAAAGAAATTTATAAGCAATATTTATGTTCATTAAATTTAATTCATTCATTGCCTAATTATGGTGTATTTTGA", "aa": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLCSLNLIHSLPNYGVF", "aa_hexdigest": "40336ff1c77cc39245a7053cb8b8897b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 443559, "stop": 444125, "observations": { "mutation": [ 443864 ], "stop": [ 443864 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8C5", "query_cov": 1.0, "subject_cov": 0.41232227488151657, "identity": 0.977, "score": 167.0, "evalue": 9.5e-51, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPNYGIFRNLRDYFEKRVAQYDFKKKIVDKNEHIFSQYIEDKQDKETLAKISDDFKNLFEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEGMLRSNKTNNIDFKTLQRKFDEIKKISINDK" }, "paralog": false, "identity": 0.9627659574468085, "score": 360.0, "evalue": 1.63e-129, "description": "Internal stop codon at 443864. Nonsense mutation around 443864.", "psc": { "uniref90_id": "UniRef90_D1J8C5", "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8C5", "UniRef:UniRef50_D1J8C5" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9980.749999999993, "isoelectric_point": 10.389483070373537 }, "id": "DFOKDJOJIG_802", "locus": "GCA013367855_00791" }, { "type": "cds", "sequence": "contig_1", "start": 444366, "stop": 444776, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17770.6" ], "nt": "ATGCCTTATCACGGTGAATATAGAATGAGCGTTGTTCATGGCTATACTGCTATTGAAAGCGGTGTTGATGCTAAAAATATTTTGATTGCTAGATTAGGGGATGTTTATTATTTAGAAAATCATCAAGTTCGTTTGTCGAATGAAAAGGTTTATTTTGGGCCTGTTTTTATTGATGGCAATATTTTATCAAAAACTAACTCTCAAATTATTAAAGAACGTAGTGAATTAGGCCAAAATGGATTTATGCATGTTGTTTTAGCAATTAATAAAGAAAAAAATCTAATTATTGGTAAACCAAGAATAGTTTCTCGTGGCGCATTTTATGTAAAAAATTCATTGCATTTAATTGAAGAAGCAAAGAGATTGGTTCATGGCGCTATATTATATACAATTAAAAATTCAAATGATTGA", "aa": "MPYHGEYRMSVVHGYTAIESGVDAKNILIARLGDVYYLENHQVRLSNEKVYFGPVFIDGNILSKTNSQIIKERSELGQNGFMHVVLAINKEKNLIIGKPRIVSRGAFYVKNSLHLIEEAKRLVHGAILYTIKNSND", "aa_hexdigest": "20886ae1a00e5afdf394dd569c4a12bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17770.6", "name": "RNase_J_C", "length": 60, "aa_cov": 0.4411764705882353, "hmm_cov": 1.0, "evalue": 8.130878199354048e-14, "score": 48.14616012573242, "start": 69, "stop": 128 } ], "seq_stats": { "molecular_weight": 15400.56679999999, "isoelectric_point": 9.300031852722167 }, "id": "DFOKDJOJIG_803", "locus": "GCA013367855_00792" }, { "type": "cds", "sequence": "contig_1", "start": 444999, "stop": 445460, "strand": "-", "frame": 2, "gene": null, "product": "ribonuclease J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080D5B6", "UniRef:UniRef90_UPI003080D5B6" ], "nt": "ATGATGACAGGTGATTTTAGGTTTGACTACACACCTATTGGAAATTTTACAGATTTTAATAAACTTAAACAAATTGGCGACGAAGGCCTTGATGTTTTATTTTCTGATTCAACAAATGCTATGCGTCCTAATCATTCACCATCAGAGTCAGATATTTTAAAAGATATTAAAAAATATATGTTGGAAGCAGAAAAGAAAATCATTGTAACTGCATTTGCTTCTAATTTAACTAGAATTAAAGCAATTATTGAATTAGGCGCTTCTTTAAATAAAAAGGTTGTTGCCTTCGGCCGTTCTATGGTTGATGGAATAGATATTGGTAGAAGACTAGGTTATATTAATGTTCCAGATGAAGTGTTTATTGACAAAAAATCATTAAGCAAATTTGGAGATAATGAGTTATTAATTTTAACAACTGGTTCTCAAGGTGAAGAACTAGCCGGACTTGCAAAGATGTCATGA", "aa": "MMTGDFRFDYTPIGNFTDFNKLKQIGDEGLDVLFSDSTNAMRPNHSPSESDILKDIKKYMLEAEKKIIVTAFASNLTRIKAIIELGASLNKKVVAFGRSMVDGIDIGRRLGYINVPDEVFIDKKSLSKFGDNELLILTTGSQGEELAGLAKMS", "aa_hexdigest": "e42de43bff8d4d1683c236ec3a4bfa47", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 445044, "stop": 445592, "observations": { "mutation": [ 445526, 445556 ], "stop": [ 445526, 445556 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI003080D5B6", "query_cov": 0.9019607843137255, "subject_cov": 0.7540983606557377, "identity": 0.855, "score": 233.0, "evalue": 2.82e-76, "gene_start": 1, "gene_end": 138, "reference_start": 46, "reference_end": 183, "reference_sequence": "TNVEFIEINKDLVVKFGECSDDWWTAQHSIPDAFGVRVKTPTGSLMLTGDIRFDYTPIGNLTDLSKLMKIGEEGLDVLFSDSTNAMRPNHSPSASDILKDIKKYMLEAEKKSIVTAFASKLTRIKAIIELGASLGKKVIAFGRSMIYNIDIGRRLGYINAPDEVFIDKKHLSKYNDNELLILT" }, "paralog": false, "identity": 0.8131868131868132, "score": 295.0, "evalue": 4.62e-103, "description": "Internal stop codon at 445526, 445556. Nonsense mutation around 445526, 445556.", "psc": { "uniref90_id": "UniRef90_UPI003080D5B6", "product": "ribonuclease J", "uniref50_id": "UniRef50_UPI003080D5B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI003080D5B6", "UniRef:UniRef50_UPI003080D5B6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI003080D5B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080D5B6" ], "product": "ribonuclease J" } }, "id": "DFOKDJOJIG_804", "locus": "GCA013367855_00793" }, { "type": "cds", "sequence": "contig_1", "start": 445554, "stop": 445925, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00753.33" ], "nt": "ATGAGCCCAACTAAAATTTTTGGATTAGGCGGAATGCAAGAAATTGGTAAAAGTACTTTGGTTATTCAATATGAAAATCATATTTTTATTATTGACACCGGAATTAAATTTTGCGATTCATATGTAACAGGTGTAAATGGTTCTATTCCAGATTTCACTTTTTTAGCAGAAAATCAAGATAAAATCGAAGGTCTTTTCATAACCCACGGACATGAAGATCATATTGGAGGTGTTCCATATTTAGTTCAACAAGTAGATGTTAAAAAAATATTTGCACCTAGAATTGCTATTCAATATCTAAAACTAAAATTTGAGGATATGAAGATAAAACGTAATATTGAATTTATTGAAACTGAAAAAGATGCGGTTTGA", "aa": "MSPTKIFGLGGMQEIGKSTLVIQYENHIFIIDTGIKFCDSYVTGVNGSIPDFTFLAENQDKIEGLFITHGHEDHIGGVPYLVQQVDVKKIFAPRIAIQYLKLKFEDMKIKRNIEFIETEKDAV", "aa_hexdigest": "5657dcf5c352ec4d827c4c65c5ef9a20", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00753.33", "name": "Lactamase_B", "length": 77, "aa_cov": 0.6260162601626016, "hmm_cov": 0.9090909090909091, "evalue": 6.241430539457537e-12, "score": 41.480987548828125, "start": 18, "stop": 94 } ], "seq_stats": { "molecular_weight": 13935.941499999986, "isoelectric_point": 5.498797416687012 }, "id": "DFOKDJOJIG_805", "locus": "GCA013367855_00794" }, { "type": "cds", "sequence": "contig_1", "start": 446262, "stop": 446471, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTATTATTCAAAATAGTGATATTATTCTAACTATTTCACATAATAATAGAATTGGCAAACATAATGTAACAATAATAAGTATTATTGGAACTTTGTGCAATGATCAACTAGCTTTTGCTATTACTAACATGCTAAATATAAAGCAAACATATAACAATATTGGCAATGCTAAAATTGCACTAATTAAAACTTTATTATTTTTATAA", "aa": "MIIIQNSDIILTISHNNRIGKHNVTIISIIGTLCNDQLAFAITNMLNIKQTYNNIGNAKIALIKTLLFL", "aa_hexdigest": "3f8cf958acade0ceadde89695f902ec8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7678.047099999998, "isoelectric_point": 9.515485191345213 }, "id": "DFOKDJOJIG_806", "locus": "GCA013367855_00795" }, { "type": "cds", "sequence": "contig_1", "start": 446601, "stop": 447536, "strand": "-", "frame": 2, "gene": "trxB", "product": "Thioredoxin reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "RefSeq:WP_175972292.1", "SO:0001217", "UniParc:UPI001589E3F5", "UniRef:UniRef100_UPI001589E3F5", "UniRef:UniRef50_A0A448ZW45", "UniRef:UniRef90_D1J8C8" ], "nt": "ATGGAAAATAATAACTTATATGATGTTTTAATAATTGGGGCAGGGCCAGCAGGATTAACGGCTGCAATATATCTAGCAAGAAATGACATAAATGTTGCTTTTATTGAAGGCGGAGTACCAGGCGGAAAAGTTTCTTCTCAATCTAAAATTGAGAATTATCCAGGTTTTAGTTTTATAAGTGGTTTTGAACTTTCTAAAGCTATGCTTAAACAAGCAAGAGATAATGGCTCAAAATTTATTTACGGTAATGTAAAGGAAATTAATTCTATATCAGATATTGAAAAAGAAGTCATTTTGGAAAATGGCATGTCAATAAAATCAAAAGCAATAATTATTGCAACCGGCATGAAAGAAATTGTTCCATTGGACGTAAAAGGCATTGAAGAATATAGAGGAAAAGGCGTATCATATTGCGTAATTTGCGATGGCCCTTTATATAAAAATAAACCTTGTGCAATCATTGGTGGTGGTAATTCGGCATTCGAAGAATCATCTTATTTAGCATCAATTGCTTCTGAGGTTCATATTTTTGTTCGTGATGGAATTATTGCAGAAAAAAAACTTGTCAATGAAGCAATGTCTAAAAGTAATATTTATATTCATGAAAATTCAACTATTAAAGAAATTTTGGGCAATGAAAAAGTTGAAGAAATTATTGCAAATGTAAATGGAAAAGAAACAAAAATGAATATAAGTTGCATTTTTCCTTATGTTGGTTTTAAACCTAACACAGCATTTATAAAAGGCAAATACGATGAAATATTAAATAGCCGTGGATTTATTGAAGTTAATGAAAATATGGAAACTAAAATTCCTAATATTTTTGCAATAGGCGATGTTATTGCAAAAGATGTAAGACAAATTACAACAGCAACAGCTGATGGGACAATTGCTGCAAGAGCAATAAGCAATAAAATCGATAAATTATATAAATAA", "aa": "MENNNLYDVLIIGAGPAGLTAAIYLARNDINVAFIEGGVPGGKVSSQSKIENYPGFSFISGFELSKAMLKQARDNGSKFIYGNVKEINSISDIEKEVILENGMSIKSKAIIIATGMKEIVPLDVKGIEEYRGKGVSYCVICDGPLYKNKPCAIIGGGNSAFEESSYLASIASEVHIFVRDGIIAEKKLVNEAMSKSNIYIHENSTIKEILGNEKVEEIIANVNGKETKMNISCIFPYVGFKPNTAFIKGKYDEILNSRGFIEVNENMETKIPNIFAIGDVIAKDVRQITTATADGTIAARAISNKIDKLYK", "aa_hexdigest": "a675132d70b956cd775645868f668ce8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI001589E3F5", "ncbi_nrp_id": "WP_175972292.1", "uniref100_id": "UniRef100_UPI001589E3F5", "db_xrefs": [ "SO:0001217", "UniParc:UPI001589E3F5", "RefSeq:WP_175972292.1", "UniRef:UniRef100_UPI001589E3F5" ] }, "ips": { "uniref100_id": "UniRef100_UPI001589E3F5", "uniref90_id": "UniRef90_D1J8C8" }, "psc": { "uniref90_id": "UniRef90_D1J8C8", "gene": "TrxB", "product": "Thioredoxin reductase", "uniref50_id": "UniRef50_A0A448ZW45", "cog_id": "COG0492", "cog_category": "O" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZW45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZW45" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "DFOKDJOJIG_807", "locus": "GCA013367855_00796" }, { "type": "cds", "sequence": "contig_1", "start": 447570, "stop": 447827, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACCTTTACGTGATGGCAGTTATACTATTTATAAAGTAATTTCTGCTTTATATATAATAGTTGGTTTGGCATTGACTGTAATGTTTGAATTCGTTATAAAATTGAATTACAATGTTTATAAAGTTAATACATATAATAACGAAAAGCTTTCAAAATTCTTTTACTTTATAGTTTATGAACCTAAAAATTCAAACTCATTTGCATTTAAAAAATCGACTACTAAAAATCAAATAGAAACTAAAACAGCAGCTTAA", "aa": "MEPLRDGSYTIYKVISALYIIVGLALTVMFEFVIKLNYNVYKVNTYNNEKLSKFFYFIVYEPKNSNSFAFKKSTTKNQIETKTAA", "aa_hexdigest": "66f2d63094c4b84a739001b1b74c27d9", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9914.404999999988, "isoelectric_point": 9.456045341491702 }, "id": "DFOKDJOJIG_808", "locus": "GCA013367855_00797" }, { "type": "cds", "sequence": "contig_1", "start": 447909, "stop": 448145, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAAGCAATTACTATAATTTTACCAGCGGTATTAATTGGACAAGCAATTGGACGTTGGGGAAACTTCGCAAACCATGAAGTTTATGGCGGCGTTATGGAAAAGGGCTCTGTCGCATTTGAAATATTGCCAAAATTAATTAGAGATCACATGTTTATTGATGGAAAATACCGTTATCCTTTATTCTTCTATGAATCAATTGCAAACTTAGCTGCTTATATTATCTTAGTTTGA", "aa": "MKKAITIILPAVLIGQAIGRWGNFANHEVYGGVMEKGSVAFEILPKLIRDHMFIDGKYRYPLFFYESIANLAAYIILV", "aa_hexdigest": "14942c0b7e224d900c4c469552ea11dd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8817.391399999997, "isoelectric_point": 9.049249839782714 }, "id": "DFOKDJOJIG_809", "locus": "GCA013367855_00798" }, { "type": "cds", "sequence": "contig_1", "start": 448590, "stop": 450635, "strand": "-", "frame": 2, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "nt": "ATGCCAAAAATTGAACCCAAATTATTCTCGTTTAATTCTCCAGCCGGAATGTGTCCTGAATGCAAGGGACTTGGTGTTATTCAAAAGGCAAGCTTTGATTTAATTTGTCCCGATAGGCAGCTTTCTATAAACGAAGGAGCAATAAAGTATTATAGAAATTTCATGAATACTTACAATTTAGAATGGCAAGAATTTGACAAGCTATTAAATTATTATCATATTGATAAAGATCAACCTATTGATTCTTTATCTTCTTTTGAACTAGATATTATTCAACATGGGTCGCAAGAAGAAATATCATATGTGTTATTATCTGAAAGTGGCACAAAACGCGAGAAGTTTCAAGTTATTGAAGGTATTGTTGATAAATTACAAAGGAAGTATTTTGATACAAAATCACTAGAAGCTAGGGCTTATTATAGTAAATACATTGGAGACATTACTTGTCCCTCATGCCATGGAAAGCGTTTGAATAAGTATGCATTGTCTTGCAAAATTCTAGATCTAGATATATACGAAATTTGCAATAAGTCGCTTGGAGAATTGGCAATTATTTTAGAAAGATTGCAGTTAAGTGATTTAGAAAAAGAAATTACCTCTTTAATTTTGTCTGAATTGAAACATAGAATTAAATTTTTAATTGATGTTGGCTTAAAGTATTTAACATTGAATAGAAAAGCTGAGACATTAAGCGGTGGTGAAGCTCAAAGAATTAGATTAGCAACACAAATTGGCTCGAATCTAACAGGCATTTTATATGTTTTAGATGAACCATCAATTGGCTTACATCAAAAAGATAATGAAAAATTGCTAAACTCTTTAAGAAAAATGGTTGAATTAGGCAATAGTTTAATTGTTGTTGAACATGATGAAGAAACAATTAGACAAGCAGATTATATAGTTGATATTGGTATTCAAGCAGGCGAACATGGTGGAAATATTGTTGCATGTGGAAATGTTGAAAATATAATTGCATGTAAGCAAAGTCTAACTGGCCAATATTTGTCAAAAGAAAGACAAATAGAGGTGCCAAAATTTAGGCGGAGTGGAAATGGCAAGACATTAACATTAAAAAATGCAAAATTAAATAATTTAAAAAATATTAAAGTCACTTTTCCATTAGGAAAATTTATTGCAATTACTGGAGTTAGCGGTTCTGGAAAATCTACGTTAATAAACGATGAACTAGTCGGGAGTCTTAATAAATATCTATTAGATCAATATTATGATATTCAAAGAGATAATAAACTAATTGGCCAAACTAACATTGATAAATTAATTCAAATTGACCAAAATCCAATCGGGCGTACTCCTAGATCAAATCCTGCAACATATACCGGTGTTTTTGATGATATTAGAGAAATTTTTGCAAATACCGAAGAAAGTAAAATCAGAGGATATACAAAATCAAGATTTTCATTTAATGTTCCAGGTGGGCGTTGTGATAAATGTGAAGGTGATGGTGTAATCAAAATTGCTATGCATTTTTTGCCCGATGTTTATGTGACATGTGACCATTGCAACGGGAAACGTTATAATCAAGAAACGCTAGAAATAAAATATCATGGTAAAAGTATTGGCGATGTTTTGGATATGACAATCGAAAAAGCCTATGCATTATTTATAAACAAGAGCCAAATTAAAGAAAAATTGCAAGTTTTATTAGATGTTGGACTTGGCTATATTAAATTAGGCCAAAATGCTACAACATTAAGCGGTGGTGAAGCTCAAAGGGTAAAATTAGCAACATACCTACAAAAAAAGCCAACAGGCAAATCTTTATATGTATTAGATGAACCAACAACCGGATTGCATCCTTACGATATATCTAATTTATTAATAGTATTAAATAGAATAGTGGACAATGGCGATACAGTAATTGTAATTGAACATAATTTGGATTTAATTAAATGTGCTGATCATATTATTGATTTAGGCCCTGATGGTGGGGACGAAGGGGGAAAAGTTATTTGCTCAGGAACACCTGAACAGGTCTCAAAGTGTGAAAATTCGTACACGGGGCAATATTTAGCAAAACTTTTATAG", "aa": "MPKIEPKLFSFNSPAGMCPECKGLGVIQKASFDLICPDRQLSINEGAIKYYRNFMNTYNLEWQEFDKLLNYYHIDKDQPIDSLSSFELDIIQHGSQEEISYVLLSESGTKREKFQVIEGIVDKLQRKYFDTKSLEARAYYSKYIGDITCPSCHGKRLNKYALSCKILDLDIYEICNKSLGELAIILERLQLSDLEKEITSLILSELKHRIKFLIDVGLKYLTLNRKAETLSGGEAQRIRLATQIGSNLTGILYVLDEPSIGLHQKDNEKLLNSLRKMVELGNSLIVVEHDEETIRQADYIVDIGIQAGEHGGNIVACGNVENIIACKQSLTGQYLSKERQIEVPKFRRSGNGKTLTLKNAKLNNLKNIKVTFPLGKFIAITGVSGSGKSTLINDELVGSLNKYLLDQYYDIQRDNKLIGQTNIDKLIQIDQNPIGRTPRSNPATYTGVFDDIREIFANTEESKIRGYTKSRFSFNVPGGRCDKCEGDGVIKIAMHFLPDVYVTCDHCNGKRYNQETLEIKYHGKSIGDVLDMTIEKAYALFINKSQIKEKLQVLLDVGLGYIKLGQNATTLSGGEAQRVKLATYLQKKPTGKSLYVLDEPTTGLHPYDISNLLIVLNRIVDNGDTVIVIEHNLDLIKCADHIIDLGPDGGDEGGKVICSGTPEQVSKCENSYTGQYLAKLL", "aa_hexdigest": "9cda159a2d813ca178d0000733aaec5d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P0N4", "query_cov": 0.8854625550660793, "subject_cov": 0.9934102141680395, "identity": 0.738, "score": 893.0, "evalue": 0.0, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0P0N4", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "product": "UvrABC system protein A" }, "genes": [], "id": "DFOKDJOJIG_810", "locus": "GCA013367855_00799" }, { "type": "cds", "sequence": "contig_1", "start": 450685, "stop": 450867, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTCATTAACAATAGTTGAAATTAAACCATTAGATTGCTCTAATGCAACCTCAATTGCACTTGATATTCTTGATTTTATATCATCATCATTTGATAATTTAACTCTATCAATAAACAGATCAATATTTCAACGTTTATTTTTATCTAGATTAATTGAATCAACATTTTCTAAGAAATAA", "aa": "MLSLTIVEIKPLDCSNATSIALDILDFISSSFDNLTLSINRSIFQRLFLSRLIESTFSKK", "aa_hexdigest": "cd9470a13223746c5e35c9ad660c34c2", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6767.816499999995, "isoelectric_point": 6.024842262268066 }, "id": "DFOKDJOJIG_811", "locus": "GCA013367855_00800" }, { "type": "cds", "sequence": "contig_1", "start": 450816, "stop": 451418, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17760.6" ], "nt": "ATGAACGATAAAATAATAATAAAAGGTGCTAGAGAGAATAATTTAAAGAATATTAATTTAGATATTCCTCGTAACAAATTTGTGGTTTTTACTGGTGTCAGTGGCTCTGGTAAAAGTTCTTTAGCATTTAATACAATCTATGAAGAAGGCAGACGTAGGTATGTTGATAGCCTTTCTAATTATGCAAAACAATTTTTAGGTGGTACTAAAAAACCTAATGTTGATAGCATTGAAGGATTGAGCCCAGCAATTTCTATTGAACAAAAAACAGTACATGTTAATCCCAGAAGTATAGTTGGTACTGTTACTGAAATTTATGATTATTTAAGATTGCTATATGCAAGAGTAGCAAAACCTTATTGCCCAAATCACAAAATTGAAATTACTGCTCAAAAAACAAAAGACATTATTTCTAGTATTTATAAAAACCCATTAGGATCAACAATATACATATTAGCCCCATTAGTAAATAATCAAAAAGGAAGCCACCAAGTATTACTTAATAAATTAAAAAGAGATGGTTTTATAAAAGTTTTAATAAATGATGAAATTTATTTCTTAGAAAATGTTGATTCAATTAATCTAGATAAAAATAAACGTTGA", "aa": "MNDKIIIKGARENNLKNINLDIPRNKFVVFTGVSGSGKSSLAFNTIYEEGRRRYVDSLSNYAKQFLGGTKKPNVDSIEGLSPAISIEQKTVHVNPRSIVGTVTEIYDYLRLLYARVAKPYCPNHKIEITAQKTKDIISSIYKNPLGSTIYILAPLVNNQKGSHQVLLNKLKRDGFIKVLINDEIYFLENVDSINLDKNKR", "aa_hexdigest": "1b1e51d9a8dba3f147fc440075009048", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_W7DIQ3", "query_cov": 0.99, "subject_cov": 0.9611650485436893, "identity": 0.5660000000000001, "score": 231.0, "evalue": 4.38e-74, "valid": false, "product": "UvrABC system protein A", "uniref50_id": "UniRef50_W7DIQ3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17760.6", "name": "UvrA_inter", "length": 69, "aa_cov": 0.345, "hmm_cov": 0.9710144927536232, "evalue": 2.2638946827487707e-19, "score": 64.99248504638672, "start": 131, "stop": 199 } ], "seq_stats": { "molecular_weight": 22585.738300000023, "isoelectric_point": 9.641972160339353 }, "id": "DFOKDJOJIG_812", "locus": "GCA013367855_00801" }, { "type": "cds", "sequence": "contig_1", "start": 451564, "stop": 452121, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2DDAE5", "UniRef:UniRef90_UPI001E2DDAE5", "PFAM:PF07804.18" ], "nt": "GTGGATGGCGAAATTCATTTATATAAACGAAACTCAAAATTTGCAAATAAAAACTTATACGATGTTTATAGTGAATATTTTGCAAGTCAAGTTGCCAAATTTTTGAATTTACCGGCAGTTGAATATAAAATTACTAAGTATGGAAAGCATTTTGTTAGTGATTGCAAATTGTTTACAAACAAAGATTTTTCATTCATCCCATTTTCTAAAATCATAGATCCCAATAGTATTGATAATAATTTAAAACTATGTAGTCAGATTGCTTTTTATTATGGCAAAGAGTTATTTGAAGATTTAATGGTTTTTGATGCTTTAATTTGCAATGGGGATAGGCATCTAAATAATTTTGGCATGATGTTGGATAATAATAGTGGAATTTTAACAAAGCCAGCTCCAATATTTGATTTCAACAATAGTTTAATATTTGATTATCCTATTTATTTAAAATCAATTGCAAAAACTGCATTTTTAAATGACTATGAGAATCGCAAAACTAGGTTTTTTGAAAGTTTTAATCAACAACTAAAAATTTTTATTAGAAAAAGACATTTAGATTGA", "aa": "MDGEIHLYKRNSKFANKNLYDVYSEYFASQVAKFLNLPAVEYKITKYGKHFVSDCKLFTNKDFSFIPFSKIIDPNSIDNNLKLCSQIAFYYGKELFEDLMVFDALICNGDRHLNNFGMMLDNNSGILTKPAPIFDFNNSLIFDYPIYLKSIAKTAFLNDYENRKTRFFESFNQQLKIFIRKRHLD", "aa_hexdigest": "7f61af2af0c67346d3503b0e54faa4db", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 451408, "stop": 452322, "observations": { "mutation": [ 451566, 452130, 452160, 452271, 452307 ], "stop": [ 451566, 452130, 452160, 452271, 452307 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2DDAE5", "query_cov": 1.0, "subject_cov": 0.6085526315789473, "identity": 0.9840000000000001, "score": 374.0, "evalue": 2.47e-129, "gene_start": 1, "gene_end": 185, "reference_start": 68, "reference_end": 252, "reference_sequence": "MCNSFWICSQEESCKLNWEDVNYFDHFNNDSCNYFLDYKAKLTETNRLQIKSPSWFIDGDMLKAWVEVDGEIHLYKRNSKFANKNLYDVYSEYFASQVAKFLNLSAVDYKITKYGKHFVSDCKLFTNKDFSFIPFSKIIDPNSIDNNLKLCSQIAFYYGKELFEDLMVFDALICNGDRHLNNFGMMLDNNSGILTKPAPIFDFNNSLIFDYPIYLKSIAKTAFLNDYENRKTRFFESFNQQLKIFIRKRHLDWIKKLKKFKFKQPNKIKMDMKYLKFINKFFKKRIKKAEQYLIEKGYIKENER" }, "paralog": false, "identity": 0.9671052631578947, "score": 598.0, "evalue": 2.39e-220, "description": "Internal stop codon at 451566, 452130, 452160, 452271, 452307. Nonsense mutation around 451566, 452130, 452160, 452271, 452307.", "psc": { "uniref90_id": "UniRef90_UPI001E2DDAE5", "uniref50_id": "UniRef50_UPI001E2DDAE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2DDAE5", "UniRef:UniRef50_UPI001E2DDAE5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2DDAE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2DDAE5" ] } }, "hypothetical": true, "pfams": [ { "id": "PF07804.18", "name": "HipA_C", "length": 167, "aa_cov": 0.8216216216216217, "hmm_cov": 0.9281437125748503, "evalue": 6.688358244754037e-10, "score": 34.595985412597656, "start": 25, "stop": 176 } ], "seq_stats": { "molecular_weight": 21809.77020000001, "isoelectric_point": 8.616989326477054 }, "id": "DFOKDJOJIG_813", "locus": "GCA013367855_00802" }, { "type": "cds", "sequence": "contig_1", "start": 452437, "stop": 452583, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACATATTATTTAAAATGTAAAAACAGGATTTGTTTAAGTTTTGAAGCAGATGAAAAATTAACAACATTGAGCAATGTTAAATTGCTTGATGATTTTATTTTTATAAAGCAAAAATATAATGAATATTCGCCAAGACAATGA", "aa": "MKTYYLKCKNRICLSFEADEKLTTLSNVKLLDDFIFIKQKYNEYSPRQ", "aa_hexdigest": "61c5f45d2d3587c0015990319f891d47", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5840.7453000000005, "isoelectric_point": 9.002445793151857 }, "id": "DFOKDJOJIG_814", "locus": "GCA013367855_00803" }, { "type": "cds", "sequence": "contig_1", "start": 452590, "stop": 452748, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BCA40", "UniRef:UniRef100_Q8RK97", "UniRef:UniRef50_A0A454CA89", "UniRef:UniRef90_A0A454CA89" ], "nt": "ATGCTAGAAAATTTACAAAAAATATTGCACCGTGATCTTTCAAAAGAAATTGCAGAAGTTGAAGAAAAGTATAAAAATCAAGCAAAGGAAGACCACAAAGAAGATCCAAAAACAAAATCAAAAGATAATAAGAACGATTTTGATGGCGAAATAAATTAG", "aa": "MLENLQKILHRDLSKEIAEVEEKYKNQAKEDHKEDPKTKSKDNKNDFDGEIN", "aa_hexdigest": "9bddfb4c4a295b23be16d0370265a4b8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000BCA40", "uniref100_id": "UniRef100_Q8RK97", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BCA40", "UniRef:UniRef100_Q8RK97" ] }, "ips": { "uniref100_id": "UniRef100_Q8RK97", "uniref90_id": "UniRef90_A0A454CA89" }, "psc": { "uniref90_id": "UniRef90_A0A454CA89", "uniref50_id": "UniRef50_A0A454CA89" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA89" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6184.768400000002, "isoelectric_point": 5.288891029357909 }, "id": "DFOKDJOJIG_815", "locus": "GCA013367855_00804" }, { "type": "cds", "sequence": "contig_1", "start": 452758, "stop": 453180, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2C38EE", "UniRef:UniRef90_UPI001F2C38EE" ], "nt": "ATGTCAATAGAAGATTATGATGGTGGGTTTGGAACTAAGGAATTTTCTCTTGAATCTGAATTATCAGAAGACGAAGAATCTGATGAAAAATCAGATGATGGCGATTCAAATGAAGAAGAAATAGATATGAGTAGTATGGATGATTTTAAAAATTCATTTGTAAATTTCTTAGAAGGTTTTGATGATTTAAAACAACAAGCAAAAAAATTAAAAGAACAAATTGCTGATAATAAATTTATGTCTGGTTCTCCATTTGAAATGAATTATGAAGATCTAAGTATGGAAAAATTAGTTGATAGTATGTATAAAGCTGCCAAAGCAATTGGAAATGTTCGTGATGATGAAATGGTTGATTATTTCAAGGGCGCATTCGATGAATTAAGAGGAATGGGAATAAATAATATTCCTAATGACGGTTCATGA", "aa": "MSIEDYDGGFGTKEFSLESELSEDEESDEKSDDGDSNEEEIDMSSMDDFKNSFVNFLEGFDDLKQQAKKLKEQIADNKFMSGSPFEMNYEDLSMEKLVDSMYKAAKAIGNVRDDEMVDYFKGAFDELRGMGINNIPNDGS", "aa_hexdigest": "b1b7707aceb4508c427cf45fef507279", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 452590, "stop": 453253, "observations": { "insertion": [ 453196 ], "mutation": [ 453223 ], "stop": [ 452760, 453223 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2C38EE", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 1.0, "score": 262.0, "evalue": 3.12e-87, "gene_start": 1, "gene_end": 140, "reference_start": 25, "reference_end": 164, "reference_sequence": "MKFSREFIGKQNLSNRRSIPIERRMSIEDYDGGFGTKEFSLESELSEDEESDEKSDDGDSNEEEIDMSSMDDFKNSFVNFLEGFDDLKQQAKKLKEQIADNKFMSGSPFEMNYEDLSMEKLVDSMYKAAKAIGNVRDDEMVDYFKGAFDELRGMGINNIPNDGSWEKSMLENLQKILHRDLSKEIAEVEEKYKNQAKEDHKEDPKTKSKDNKNDFDGEIN" }, "paralog": false, "identity": 0.9772727272727273, "score": 400.0, "evalue": 3.55e-144, "description": "Internal stop codon at 452760, 453223. Frameshift due to insertion around 453196. Nonsense mutation around 453223.", "psc": { "uniref90_id": "UniRef90_UPI001F2C38EE", "uniref50_id": "UniRef50_UPI001F2C38EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2C38EE", "UniRef:UniRef50_UPI001F2C38EE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2C38EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2C38EE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15847.015999999989, "isoelectric_point": 4.0500284194946286 }, "id": "DFOKDJOJIG_816", "locus": "GCA013367855_00805" }, { "type": "cds", "sequence": "contig_1", "start": 453352, "stop": 453723, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2E838F", "UniRef:UniRef90_UPI001F2E838F" ], "nt": "TTGTCAGATTTAAACACTTTTAATTCCCTAAATATTTTTAATGATTTTTTAATAAATGAAGATGAAGAAAATAAAAATTCTTTTGACTTTAAAAAATATGGACTTGAACAAAATGAAATTATTTTCTCTGCAATTTTACCTACAAAAGAAATTATTTCATTGGCATTAAAAATTGATTTTGTTTATGACGATAAAAGAGAATTTGATGAAGATTTTCAAATCGATCCTGAATTAATTAGTCAAAGTGTAATTTCCGAAAGAAAAGAAACTTTAAAATATTTAAAAGAATTAAAGAAAAATGAATTTAAAAATTTTCATACAAATCCTTCAAATGATATTAACAACGTAACTAGAAGCGATTTTAGTAAATGA", "aa": "MSDLNTFNSLNIFNDFLINEDEENKNSFDFKKYGLEQNEIIFSAILPTKEIISLALKIDFVYDDKREFDEDFQIDPELISQSVISERKETLKYLKELKKNEFKNFHTNPSNDINNVTRSDFSK", "aa_hexdigest": "96cf75e0cee01bdef41ffc3594aa704d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 453383, "stop": 454011, "observations": { "insertion": [ 453416, 453429 ], "mutation": [ 453804 ], "stop": [ 453391, 453804 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2E838F", "query_cov": 0.8048780487804879, "subject_cov": 0.47596153846153844, "identity": 0.99, "score": 186.0, "evalue": 1.37e-57, "gene_start": 1, "gene_end": 99, "reference_start": 97, "reference_end": 195, "reference_sequence": "MIKKQLGINLKLTVRELNVAFDYEFKPIEAVKKINFEPISFPITSGISFSELVDVLGAFFGFEKSVVFHWYTIPNYLKTIVPFEFVFGDLQASQEFLSDLNTFNSLNIFNDFLINEDEENKNSFDFKKYGLEQNEIIFSAILPTKEIISLALKIDFVYDDKREFDEDFQIDPELISQSVISERKETLKYLKELKKMNLKFSYKSFKWY" }, "paralog": false, "identity": 0.9903846153846154, "score": 392.0, "evalue": 1.91e-141, "description": "Internal stop codon at 453391, 453804. Frameshift due to insertion around 453416, 453429. Nonsense mutation around 453804.", "psc": { "uniref90_id": "UniRef90_UPI001F2E838F", "uniref50_id": "UniRef50_UPI001F2E838F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2E838F", "UniRef:UniRef50_UPI001F2E838F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2E838F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2E838F" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14572.047899999978, "isoelectric_point": 4.605288505554199 }, "id": "DFOKDJOJIG_817", "locus": "GCA013367855_00806" }, { "type": "cds", "sequence": "contig_1", "start": 453802, "stop": 454011, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAGAAGCAATTAGGAATAAATCTAAAATTAACAGTTAGAGAATTAAATGTAGCTTTTGACTACGAATTTAAGCCAATAGAAGCAGTTAAAAAAATTAATTTTGAACCAATATCATTTCCAATAACTTCAGGGATTTCTTTTTCTGAATTAGTGGATGTTTTAGGCGCATTTTTTGGCTTTGAAAAGAGTGTTGTATTTCATTGA", "aa": "MIKKQLGINLKLTVRELNVAFDYEFKPIEAVKKINFEPISFPITSGISFSELVDVLGAFFGFEKSVVFH", "aa_hexdigest": "8369d173a9642d4d732bab994d7713c5", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7838.120199999998, "isoelectric_point": 6.552023887634278 }, "id": "DFOKDJOJIG_818", "locus": "GCA013367855_00807" }, { "type": "cds", "sequence": "contig_1", "start": 454004, "stop": 454285, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTTAGAAAATGTTTATAAGAATGTAGAAACATCTAAAGATGAAATAATGACAATCGGGGATGGAAATAATGATATACCAATGCTGTCAATATCAAAATATTCATATGCAATGGCAAATGCAACACAAACCGTACATGAACATAGCCACTTTCATACAAGTGCTGTTGAACAAAATGGTTTGGGAGAAGCAATACTTGATTACATATATAGATTAAAAAATATAACTAAAAAATATATGTTGCATGAATTTGAAAACAATAGAGGAGTAGAAAATGATTAA", "aa": "MLENVYKNVETSKDEIMTIGDGNNDIPMLSISKYSYAMANATQTVHEHSHFHTSAVEQNGLGEAILDYIYRLKNITKKYMLHEFENNRGVEND", "aa_hexdigest": "70081469e4d8032eb2acaec8be293d33", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 64, "aa_cov": 0.6881720430107527, "hmm_cov": 1.0, "evalue": 1.6289919234137505e-18, "score": 62.95213317871094, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 10682.783399999993, "isoelectric_point": 5.228414344787596 }, "id": "DFOKDJOJIG_819", "locus": "GCA013367855_00808" }, { "type": "cds", "sequence": "contig_1", "start": 454286, "stop": 454390, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAAAATTTAAGTCAATAACAAATTTGGCAATGATTCCAACAAATTGCAATATTGAAATATCACCATTAAATATTTCAAAAGGGTCTGCTGTTCTTTGA", "aa": "MFEKFKSITNLAMIPTNCNIEISPLNISKGSAVL", "aa_hexdigest": "4e9fae4fedb1f4afe08aa8406314dabb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3725.3994000000002, "isoelectric_point": 7.934720420837403 }, "id": "DFOKDJOJIG_820", "locus": "GCA013367855_00809" }, { "type": "cds", "sequence": "contig_1", "start": 454649, "stop": 454924, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAGTCAAACAATTTTAAAAGTTACTAACAACAAGAAGCCAATTGCTTTTTCAGATGTTGACGGTACTATATATAAAAATTTTAATTTAAAGCAAGAAACTATTGATGATATCCATTTTGCAGTAGACAATGGATTAGATTTTAATATATGCACCGGAAATCCAATTCAAGAAAGAATGTTAAAACTAGCCGAAACATTAAAAGCAAGATATTTAATAGGATCTTCCGGCGCTCAAATTTTTGATATGCAATCAAGAGAATATATTGCCTGTTGA", "aa": "MSQTILKVTNNKKPIAFSDVDGTIYKNFNLKQETIDDIHFAVDNGLDFNICTGNPIQERMLKLAETLKARYLIGSSGAQIFDMQSREYIAC", "aa_hexdigest": "3d09afb544d3af4ccad0b0724e2ecdca", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 75, "aa_cov": 0.8021978021978022, "hmm_cov": 0.9733333333333334, "evalue": 4.034593989933343e-10, "score": 35.44811248779297, "start": 17, "stop": 89 } ], "seq_stats": { "molecular_weight": 10276.648599999993, "isoelectric_point": 5.686195564270019 }, "id": "DFOKDJOJIG_821", "locus": "GCA013367855_00810" }, { "type": "cds", "sequence": "contig_1", "start": 454941, "stop": 455744, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175972297.1", "SO:0001217", "UniParc:UPI00158836D0", "UniRef:UniRef100_UPI00158836D0", "UniRef:UniRef50_A0A454CA04", "UniRef:UniRef90_A0A454CA04" ], "nt": "ATGAACAAAAAACAAATAAAAAAATTCTTGGGTTTATCATTGACTATTCCAACTTTACTAACTATCCCATTAGTTTCACTGTCATGCAAAAAATCAAAAGATAATCAAGATAATTTAGAATTAGGCAATGATGATGGAATTGAAAAACAAGATTTAAGTGTTGCCAAAAGAATAGTTGAAATATTTAAAGAACACAGATTTTCATTTGATGAAATGAATCAAAAATTATTCTTACAAAATCTAAATATAGAAGACCCTAAAAATCCATTTAAAAAAATAAGATTTGAAGATTTTTATAACAATAGAGTAAAAGATTGGAATTTTAAAGAAAAGTCTGAATACAATAGAGCTTTGGAGTTGGATAATGATCTTAAATATCCCTTCTTTGATATAAAGAATCAAAATAGTTTTATTAATTTATTAAAAAATGATCCTGATCCTAATCTTTATAGAGTTTTTAATAAATATTTCCAAATAAGATTAAGAACAAATGACGAACTTTTAAAAATACTTTTTGTGTTTGAATTAGTATCAAAAAAAATGCAACAACAATTAAGCAATGAAATAAAAAAAGATGCCTCGGATTTAAATGTAAAGAATTATTTCAATTTAAAAACATATGCCATAAATCATTTTTTATTAGATTATACGGAATTTCTAAAATCAGAAGAACCCGAAACTACAATGCATTATACATTTAGACTATTAAGCTATATTGTGCCACCAGTTGCCATTTCTGGCATTATAATTTATATTATTATAATGGTATTAAAAAAGAAGCATAAATCAAAGGCAAGAGCATAA", "aa": "MNKKQIKKFLGLSLTIPTLLTIPLVSLSCKKSKDNQDNLELGNDDGIEKQDLSVAKRIVEIFKEHRFSFDEMNQKLFLQNLNIEDPKNPFKKIRFEDFYNNRVKDWNFKEKSEYNRALELDNDLKYPFFDIKNQNSFINLLKNDPDPNLYRVFNKYFQIRLRTNDELLKILFVFELVSKKMQQQLSNEIKKDASDLNVKNYFNLKTYAINHFLLDYTEFLKSEEPETTMHYTFRLLSYIVPPVAISGIIIYIIIMVLKKKHKSKARA", "aa_hexdigest": "2b67b58d1daf498d37512fb6c58160c0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158836D0", "ncbi_nrp_id": "WP_175972297.1", "uniref100_id": "UniRef100_UPI00158836D0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158836D0", "RefSeq:WP_175972297.1", "UniRef:UniRef100_UPI00158836D0" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158836D0", "uniref90_id": "UniRef90_A0A454CA04" }, "psc": { "uniref90_id": "UniRef90_A0A454CA04", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_A0A454CA04" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA04", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA04" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "DFOKDJOJIG_822", "locus": "GCA013367855_00811" }, { "type": "cds", "sequence": "contig_1", "start": 456071, "stop": 456694, "strand": "+", "frame": 2, "gene": null, "product": "p50 adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O53103", "UniRef:UniRef90_O69453" ], "nt": "ATGAAAAAATCAAAGAAAATATTTATAACATTATGTGGAATAGCAACTACCGCTATTTTGCCAGTTGCTACTATTTCATGTAATGATGATAAACTAGCAGAGAAAAATGGAAAAGAAAAAGCAGATGCTGCTTTAAAACAAGCAAATGCATTAGCAGAAGAATTGAAAAAAAATCCTGATTATTCAAAAATTTTAGAAACATTAAACAAAGAAATTGCCGAAGCAACCAAATCATTTAAAGAAGCAGGTTCATACGGCGATTATCCAGCAATAATTTCTAAACTTTCAGCAGCAGTAGAAAATGCTAAGAATGAACAACAAAAAGTTGATCAAGCAAATAAAAAAATTGCTGATGAAAATCAAAAAATTAAAGAAGGCGCTAAAGAATTATTAAAATTAAGCGAAAAAATTCAAAGTTTTGCTGATACAATTGCATTAACAATTACTAAATTAGAAGGAAAAAAATTCCAAATTGATGAAACATTTAAAAAACAATTGATTTCAACAATTGAATTATTAAATAAAAAATCAGTCGAAGTTAAAACTTTTGCAACAGTAAATACCATTAAAAAAGATTTCCTTTTATCAGAACTAGAATCATTTAAAGAATTTAACACCTCATGA", "aa": "MKKSKKIFITLCGIATTAILPVATISCNDDKLAEKNGKEKADAALKQANALAEELKKNPDYSKILETLNKEIAEATKSFKEAGSYGDYPAIISKLSAAVENAKNEQQKVDQANKKIADENQKIKEGAKELLKLSEKIQSFADTIALTITKLEGKKFQIDETFKKQLISTIELLNKKSVEVKTFATVNTIKKDFLLSELESFKEFNTS", "aa_hexdigest": "a656da8a64f552d25f87bcf6a6f65d91", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_O69453", "query_cov": 1.0, "subject_cov": 0.8023255813953488, "identity": 0.971, "score": 337.0, "evalue": 3.53e-115, "valid": true, "product": "p50 adhesin", "uniref50_id": "UniRef50_O53103" }, "pscc": { "uniref50_id": "UniRef50_O53103", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O53103" ], "product": "Vaa surface lipoprotein adhesin" }, "genes": [], "id": "DFOKDJOJIG_823", "locus": "GCA013367855_00812" }, { "type": "tRNA", "sequence": "contig_1", "start": 457897, "stop": 457973, "strand": "-", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 59.6, "nt": "GCGTTTGTGGCGAAGTGGTTTAACGCTCCGGGTTGTGGCTCCGGCATACGTGGGTTCGATCCCCATCAAACGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 457965, 457967 ], "id": "DFOKDJOJIG_27", "locus": "GCA013367855_00813" }, { "type": "cds", "sequence": "contig_1", "start": 458164, "stop": 458451, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGCCCTAAAAGTAGAAACAAGCAAAATAAACAATTTAAATGAATTAATTAAATTATTTTATACATATTGGAAACAATTTAACTATTTAATTTCTGATAAACATGATCAAAGAGAAGTCTTTAAAAGAGGAATTAAAGATGCTGATGAAGCAATAAATGCCTTGGGACAAATTAATTTAAATCAAGACCTTGGTAATGATAAATTAATGAAAGAGAAAATCAAAGAAATTGAAAATGAACTAGCAACGGCTCAATCTTTGACAGAAAGCCATTTCAAACATTAA", "aa": "MDALKVETSKINNLNELIKLFYTYWKQFNYLISDKHDQREVFKRGIKDADEAINALGQINLNQDLGNDKLMKEKIKEIENELATAQSLTESHFKH", "aa_hexdigest": "333cf143d3a77b747f6c85f2cc246a05", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11140.498599999992, "isoelectric_point": 5.890474891662596 }, "id": "DFOKDJOJIG_824", "locus": "GCA013367855_00814" }, { "type": "cds", "sequence": "contig_1", "start": 458512, "stop": 458997, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGAGAAAAAGCCTATTGCCCATAGAATTAAATTCCGTATTGAAAATCTTAAACAAATTAAAGCCAATGAATTTTATAGCGTTTTCCTTGATAAATTTAAAAGAGTATTAGAAAAACCTATTTTCAATAATCCATCTTGGACAAAAAAATTATCAGATCTCAAGAAAGCAGATGAAGATTCAGATAATCTTTGGAAACTTTGTCAATTGTTTAGCCAATATAAAAACGACACTAATTTGTCGCCGGCTTTACAAAAAAAACTTGCAAAAGAATTTGAAAAAATATTAGCCAAAGAAACAATACCTAGTGATTCTGAAATAACTGAATTGGAAAACAAATTAAAAAAGATTGTTGAAGAAGATCCAACAACGGAATTTAGTAACCTTCAAAATGAAGCAAAAGATACTAAAAAATTAATTGAAAATCTAGTTCCTGAATCAGCTAAAGAACTTGATGGATTCAAAAAACTTCAATCATGA", "aa": "MEEEKKPIAHRIKFRIENLKQIKANEFYSVFLDKFKRVLEKPIFNNPSWTKKLSDLKKADEDSDNLWKLCQLFSQYKNDTNLSPALQKKLAKEFEKILAKETIPSDSEITELENKLKKIVEEDPTTEFSNLQNEAKDTKKLIENLVPESAKELDGFKKLQS", "aa_hexdigest": "27f97d86519d626d54f5f553d6ceded0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18876.416099999995, "isoelectric_point": 7.701308250427247 }, "id": "DFOKDJOJIG_825", "locus": "GCA013367855_00815" }, { "type": "cds", "sequence": "contig_1", "start": 459007, "stop": 463422, "strand": "-", "frame": 1, "gene": null, "product": "Variable membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9Y3", "UniRef:UniRef90_Q845L8" ], "nt": "ATGGATGAAAAAAATAAATTAAATCCCTTATATGCAAAAGAAAAATTTGAAAAAATTCAAAAACAATTGCAAGATTTAATCAATTCAGAAGATGCAAAATTCATCGATACTAATAAAGCATCCGAAACATTAAAAAATACTAAAATAACAAGCACTTCTTCAATAGATGAAATAAACCAAGCAATTAAAACTCTTAAAGATGCTATAGTTGAATTAAAAAATGCAATAAATGGTGCCAAAGAAGATTCAATAAAAGAATTTGATAAAGTTAAAAAAGATTTAGAAGTTTTAGTGGGCTCGGAAGATGCAAAACCAATTGAAAAAAGAACTGAAATTGGTATTTTGGCAAACACTTATATTCTTGAAAGAGATACAATAAAACAAATTAAAGAAAAGACTGAAGCAATTAAAAAAGCAATATCATCTTTAAACAAAAAAATCGCAAACGTTAAGGCATTAAAAAATGAGTTGCAAAAATTTAATGATATCAAGGCTGCTTTAGAACAATTAATTAAAACTGATGATGCAAAAGAAGTTGGAACAGAAAATGCATCAAAAGCTCTTAATAATAATAAAGTAAATGAAAATTCAACTCTTGAAGAAATAACAAAGGCAACTAAAGCACTTGAAGATGCAAAATCTAAATTAGATCAAGAAATCAAGACTAAAAAAGAAACTGAATTTAATAATTTAACAGTTGAAAAAGGCAAATTAAATGAATTAATTGCTAAATCAACTGATGCGTCAGCAAAAGCAATTGAAGAAGCCAAAAAAGTATTGACAGAAGTTGAAAAATTAAATAACTCCTCACCAATTAATTTACTAAAAAATGCAGTTATAAAAACAAAGGACGCCATAGAAAAACTAAGCAATAATGTTGCCCAAGAAAAAGACAAAAAAGCCAAGTTAGCTGAATTTAATAGTATAAAGACACAATTAGAAGAATTGATTGCAAAAGATGATGCAATTCAAGCTGGAGTAAACGAAGCTAAAAAAGCATTGCAAGATAATAAAGCTGATGAAAATTCAACTCTTGAAGAAATAACAAACGCAACCAAGGCATTGCAAGAAGCAAAAGCAGCATTGGAACTAAAAATTTCAAAGGCTCAAGAAAAAGCTAAACAAGAATTTGATACTAAAAAAGAACAATTAAAAACATTAATTGGCTTAAGTGATGCAAATAATGTTGATAAAAGCAATGAATTAAATGTTTTAACTAATACTTCAATTTCTAAAACAGATTCTATTAAAGAAATTAAACAAAAAACTTCAACTATCGAAGAAGCAATTAAGACCTTAAATAAAAAAGTTCAAGATAAAAAGAACGAAAAAATTCAAGAATTTGAAAAGGCTAAAGAAAGTCTAAAAAAACTAGTTAAAGAAGAAGATGCAAAAGAAGTTGGCATTGATAGTGCAAACGAAGCTTTAAAAACAAAAGTTGATGAAAAGTTAACTATTGAAGAAATTAAAAATGCTATTTTAAAAATATCTAACGAAGAAACAAAATTAAAAAGTAGCATTGAAAGTGCAAAACAACAAGCTAAAGAAACATTTAATTCAAAGAAACAAGAATTAAAAAGCCTTTTAGATTCTACAACATCTGAAGTTGATAAAAAAGCCGAAGAAGAAAAATACAATAAAACTACAATTGATAATGATTCTACAATTAAACAAATTAAACAAAAAACAAGCGACATCGAAAATGCAATATCAACCTTAAAAGAAAAAATAGCCAAAGCAAAAGGAGATAAGGCTGTTGAATTACAAAAATTTAATCTAGTAAAACAACAATTAGAAGAATTAATTGCAAAAGAAGATGCAAAAGAAGTTGGAACTTCTAAGGCTGAAGAAGCATTGAAAGTTAATGTAGCAGACGAAAATTCAACTCTTGATGCTATTTCAAAAGCAACTAAAGCTCTTGAAGACGTTAAGGCTGAATTAACACAAAATATTACTGATGCTAAAGACAATGCAACAAAAACATTCAATGACAAGAAAGATGAATTAAAGAAATTACTAGAATCAAGTGATGCCAAAATTGTTGATAATAAAAAAGAATCTGATGTTTTAAATGGCAATTCAGTTGATTCAAATACTCCAATAAAAGAAATAAAAGCCAAAACAGAAAAAATTACAGAAGCAATTAATTCTTTGACTACAAGTATAAACAATAAAAAGGATGATGAATTTAATAAATATAATGCTATTAAAACATCATTAGAAAACTTAATCAAAGAAGAAGACGCAGTTCAAGTGGGCATAGCCGATGTTCAAAAAACCTTATCCGAAAACGATGTAGATAAAACTGCAACTATTGAAAAAATTCAACATTCAACCGAAGCATTAACTCATGTCAAAGAAGAATTACAAAGATTAATTGACAATACAAAAAAACAATTAACTAAAGAATTTGAAAACAAAAAATCTGAATTAGAAAAATTAATTTCCTTACCAGATGCAAATAATGTTGATAAAAAGGATGAATTGAGCATATTCGGCAATATCAACATTACTAATAGTGATTCTATTAAACAAATAAAAGAAAAAATAACAAAAATCCAAAATGCTCTTAAATCATTAACCGATAAAATTTCAAAACAAAAAGAACAAGAATTAGAAAAATACAATACAACCAAAACAGCGCTAGAACAATTAATTAAAGATGAAGATGCAAAAGAAGTCGACACAACCGAAGCTACTACAGCATTAACTAAAACCAAAGCTGATAAAAATTCAACTCTTGAAGAAATAGCAAATGCAACTAAAGCCCTTGAAGATGCAAAATCTAAATTGGATCAAGAAATCAAGACTAAAAAAGAAGCTGAATTTAATAATTTAACTAACGCAAAAACAGAATTAAGTAATTTAATTACCTCCTCAAGTAATCAAGCGCCAGATGAAGCAATATCTGATGCTCAAAAAACATTGGATGAAATTAATAAATTAAACTTAACTAATGTTTCAACAATAAAATCAATGAAAGATGCAACTCAAAAAATTAAAGATGCAAACGAAGCATTAAAACAAGCTATAGAAAAATTAGAAGCAGAAAAAACCGAAAAATTGCAAAAATTCAACGAAGCAAAAAATGCCTTAGAAAACTTAGTTAAAGAAGAAGATGCAATTCAAACTGGAGTAGAAGCAGCTAAAAAAGCCTTGGATGACAATAATAAGATTAATAAAAATTCAACTCTTGAAGAAATTACAAGCGCAACTAAAGCACTTAAAGATGCAAAATCTAAATTAGATCAAGAAATCAAGGCTAAAAAAGAAGCTGAATTTAATAATTTAACTAATGCAAAAACAGAATTAAGTGATTTAATTACTAAACAAGCACCAGCTGAAGCAATATCTAATGCTAAAAAAGTATTAGAAGAAATTAACGGTTTAAGTCTAAGCAATGATTCCTCAATAAAATCATTGAAAGATGCAACTCAAAAAATTAAGGACGCAGAAACACAACTAACTAAAGAAATTGAAAAATTAAAAGCAGAAAAAACCGAAAAATTACAAAAATTCAACGAAGCAAAAACTGCCTTAGAAAACTTAGTTAAAGAAGAAGATGCAATTCAAACTGGAGTAGAAGCAGCTAAAAAAGTTTTAGAAGACAACAATGCTAACGAAAACTCTACCCTTGATGAAATTACAAAGGCAACTAAAGCACTTGAAGATGCAAAATCTAAATTAGATCAAGAAATCAAGACTAAAAAAGAAGCTGAATTTAATAATTTAACTAACGCAAAAACAGAATTAAGTAATTTAATTGCATCTAATAATAAACAAGCACCAGCAGATGCAATATCTGCTGCTCAAAAAACATTGGATGAAATTAATAAATTAAACTTAACTAATGTTTCAACAATAAAATCATTGAAAGATGCAACTCAAAAAATTAAAGATGCAAACGAAGCATTAAAACAAGCTATAGAAAAATTAGAAGCAGAAAAAACTGAAAAATTGCAAAAATTCAACGAAGCAAAAAAAGCTTTAGAAGATTTAATTAAAGATGAAGATGCAAAAGAAGTTGGAACAAATGATGCCCAAAAGTTGTTAGAAGATAATAACAAGATTAATAAAAATTCATCTATTGACGAAATTACAAATGCAACTAAAGCACTTGATGAAGCAAAATCTAAATTAGGCCAAAAAATCAAGGCTAAAAAACAAGAATTGATGGCTAGTTTGAAAGAAAAGAATGAAAAATTAGATACGTTATTAAAAACTGAAAATTTAGACGATCTTATGGTTATTAGCAAAAGACCTGATGATTTAAATGCGGATGAAATAAAAAATAAGCTTGAAAAAGCTAGAAAATTATCGCAAGAAGCGAGAGACCTTTCTGAAAATTCAAAAATTACTGATATAAACCAAAAAATTCAAAAAATAGATGAATTTGTTGGAAAACTTGAATCTTGA", "aa": "MDEKNKLNPLYAKEKFEKIQKQLQDLINSEDAKFIDTNKASETLKNTKITSTSSIDEINQAIKTLKDAIVELKNAINGAKEDSIKEFDKVKKDLEVLVGSEDAKPIEKRTEIGILANTYILERDTIKQIKEKTEAIKKAISSLNKKIANVKALKNELQKFNDIKAALEQLIKTDDAKEVGTENASKALNNNKVNENSTLEEITKATKALEDAKSKLDQEIKTKKETEFNNLTVEKGKLNELIAKSTDASAKAIEEAKKVLTEVEKLNNSSPINLLKNAVIKTKDAIEKLSNNVAQEKDKKAKLAEFNSIKTQLEELIAKDDAIQAGVNEAKKALQDNKADENSTLEEITNATKALQEAKAALELKISKAQEKAKQEFDTKKEQLKTLIGLSDANNVDKSNELNVLTNTSISKTDSIKEIKQKTSTIEEAIKTLNKKVQDKKNEKIQEFEKAKESLKKLVKEEDAKEVGIDSANEALKTKVDEKLTIEEIKNAILKISNEETKLKSSIESAKQQAKETFNSKKQELKSLLDSTTSEVDKKAEEEKYNKTTIDNDSTIKQIKQKTSDIENAISTLKEKIAKAKGDKAVELQKFNLVKQQLEELIAKEDAKEVGTSKAEEALKVNVADENSTLDAISKATKALEDVKAELTQNITDAKDNATKTFNDKKDELKKLLESSDAKIVDNKKESDVLNGNSVDSNTPIKEIKAKTEKITEAINSLTTSINNKKDDEFNKYNAIKTSLENLIKEEDAVQVGIADVQKTLSENDVDKTATIEKIQHSTEALTHVKEELQRLIDNTKKQLTKEFENKKSELEKLISLPDANNVDKKDELSIFGNINITNSDSIKQIKEKITKIQNALKSLTDKISKQKEQELEKYNTTKTALEQLIKDEDAKEVDTTEATTALTKTKADKNSTLEEIANATKALEDAKSKLDQEIKTKKEAEFNNLTNAKTELSNLITSSSNQAPDEAISDAQKTLDEINKLNLTNVSTIKSMKDATQKIKDANEALKQAIEKLEAEKTEKLQKFNEAKNALENLVKEEDAIQTGVEAAKKALDDNNKINKNSTLEEITSATKALKDAKSKLDQEIKAKKEAEFNNLTNAKTELSDLITKQAPAEAISNAKKVLEEINGLSLSNDSSIKSLKDATQKIKDAETQLTKEIEKLKAEKTEKLQKFNEAKTALENLVKEEDAIQTGVEAAKKVLEDNNANENSTLDEITKATKALEDAKSKLDQEIKTKKEAEFNNLTNAKTELSNLIASNNKQAPADAISAAQKTLDEINKLNLTNVSTIKSLKDATQKIKDANEALKQAIEKLEAEKTEKLQKFNEAKKALEDLIKDEDAKEVGTNDAQKLLEDNNKINKNSSIDEITNATKALDEAKSKLGQKIKAKKQELMASLKEKNEKLDTLLKTENLDDLMVISKRPDDLNADEIKNKLEKARKLSQEARDLSENSKITDINQKIQKIDEFVGKLES", "aa_hexdigest": "51fb6bb074612140bf6ef60522ad32bf", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 458704, "stop": 463722, "observations": { "deletion": [ 461994, 462027, 462042 ], "mutation": [ 459006, 463518 ], "stop": [ 459006, 463518 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q845L8", "query_cov": 0.9809653297076818, "subject_cov": 0.6658975542224274, "identity": 0.972, "score": 2085.0, "evalue": 0.0, "gene_start": 1, "gene_end": 1442, "reference_start": 415, "reference_end": 1857, "reference_sequence": "MNKKIGILLSISLLATAATTATIAAIAAKGKFKKQQELKQNIEKSKAELNKAKSILEKLLKSDYAKNVNKLEESKILDNAKLNENSSLQEIKNKTKQVKEAINSLTQKILERKQNEQQNQNDKKTLDKLRLDFANVKEELQALINSKDGKSVDTTKATEVLKNTKITNSSAAFQVLQAVQTIKKATKELREAIKFKKQQEFNEFNKIKQELTDYIANDLNDLKYKDIKAKAEKSIEKVSDININSSFISDIQQATKLLIDAKKQAISEVEKINKSSELDKYKIAKNKLELLIKTEDAQMIKSANEKAKKILAENTTKDTTLSSEEFKTRRINLENALDELKEAIKNEKNIQKQEFEAKKQELKTLLESEWSKDIDKKNETTVWNNAKIDDSDSIKEIKEATKEVQGAIDSLIQKMDEKNKLNPLYAKEKFEKIQKQLQDLINSEDAKFIDTNKASETLKNTKITSTSSIDEINQAIKTLKDAIVELKNAINGAKEDSIKEFDKVKKDLEVLVGSEDAKPIEKRTEIGILANTYILERDTIKQIKEKTEAIKKAISSLNKKIANVKALKNELQKFNDIKAALEQLIKTDDAKEVGTENASKALNNNKVNENSTLEEITKATKALEDAKSKLDQEIKTKKETEFNNLTVEKGKLNELIAKSTDASAKAIEEAKKVLTEVEKLNNSSPINLLKNAVIKTKDAIEKLSNNVAQEKDKKAKLAEFNSIKTQLEELIAKDDAIQAGVNEAKKALQDNKADENSTLEEITNATKALQEAKAALELKISKAQEKAKQEFDTKKEQLKTLIGLSDANNVDKSNELNVLTNTSISKTDSIKEIKQKTSTIEEAIKTLNKKVQDKKNEKFREFEKAKESLKKLVKRRRCKKKLALIVQTKLLKTKVDEKLTIEEIKNAILKISNEETKLKSSIESAKQQAKETFNSKKQELKSLLDFTTSEVDKKAEEEKYNKTTIDNDSTIKQIKQKTSDIENAISTLKEKIAKAKGDKAVELQKFNLVKQQLEELIAKEDAKEVGTSKAEEALKVNVADENSTLDAISKATKALEDVKAELTQNITDAKDNATKTFNDKKDELKKLLESSDAKIVDNKKESDVLNGNSVDSNTPIKEIKAKTEKITEAINSLTTSINNKKDDEFNKYNAIKTSLENLIKEEDAVQVGIADVQKTLSENDVDKTATIEKIQHSTEALTHVKEELQRLIDNTKKQLTKEFENKKSELEKLISLPDANNVDKKDELSIFGNINITNSDSIKQIKEKITKIQNALKSLTDKISKQKEQELEKYNTTNTALEQLIKDEDAKEVDTTEATTALTKTKADKNSTLEEIANATKALEDAKSKLDQEIKTKKEAEFNNLTNAKTELSNLITSSSNQAPDEAISDAQKTLDEINKLNLTNVSTIKSMKDATQKIKDANEALKQAIEKLEAEKTEKLQKFNEAKNALENLVKEEDAIQTGVEAAKKALDDNNKINKNSTLEEITSATKALEDAKSKLDQEIKAKKEAEFNNLTNAKTELSDLITKQAPAEAISNAKKVLEEINGLSXSNDSSIKSLKDATQKIKDAETQLTKEIKKLKAEKTEKLQKFNEAKTALENLVKEEDAIQTGVEAAKKVLEDNNANENSTLDEITKATKALEDAKSKLDQEIKTKKEAEFNNLTNAKTELSNLIASNNKQAPADAISAAQKTLDEINKLNLTNVSTIKSLKDATQKIKDANEALKQAIEKLEAEKTEKLQKFNEAKKALEDLIKDEDAKEVGTNDAQKLLEDNNKINKNSSIDEITNATKALDEAKSKLGQKIKAKKQELMASLKEKNEKLDTLLKTENLDDLMVISKRPDDLNADEIKNKLEKARKLSQEARDLSENSKITDINQKIQKIDEFVGKLESWIKVMEEEKKPIAHRIKFRIENLKQIKANEFYSVFLDKFKRVLEKPIFNNPSWTKKLSDLKKADEDSDNLWKLCQLFSQYKNDTNLSPALQKKLAKEFEKILAKETIPSDSEITELENKLKKIVEEDPTTEFSNLQNEAKDTKKLIENLVPESAKELDGFKKLQSWINNALKLIEEGYNSALQKEDLDALKVETSKINNLNELIKLFYTYWKQFNYLISDKHDQREVFKRGIKDADEAINALGQINLNQDLGNDKLMKEKIKEIENELATAQSLTESHFKH" }, "paralog": false, "identity": 0.9814704124327556, "score": 2669.0, "evalue": 0.0, "description": "Internal stop codon at 459006, 463518. Frameshift due to deletion around 461994, 462027, 462042. Nonsense mutation around 459006, 463518.", "psc": { "uniref90_id": "UniRef90_Q845L8", "product": "Variable membrane protein", "uniref50_id": "UniRef50_A0A454C9Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q845L8", "UniRef:UniRef50_A0A454C9Y3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9Y3" ], "product": "Variable membrane protein" } }, "id": "DFOKDJOJIG_826", "locus": "GCA013367855_00816" }, { "type": "cds", "sequence": "contig_1", "start": 463516, "stop": 464664, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019393273", "UniRef:UniRef90_UPI0019393273" ], "nt": "ATGAATAAGAAAATAGGTATTTTACTTTCAATTTCTTTGTTAGCCACAGCAGCTACAACTGCTACTATAGCAGCCATTGCCGCAAAAGGAAAATTTAAAAAACAACAAGAATTAAAGCAAAATATTGAAAAATCTAAGGCCGAACTAAATAAGGCCAAATCTATTTTAGAAAAATTACTAAAATCAGATTATGCTAAAAATGTTAATAAATTAGAAGAATCTAAAATTTTAGATAATGCTAAATTAAATGAAAATTCTTCGCTTCAAGAAATTAAAAATAAAACGAAGCAAGTAAAAGAAGCAATTAATTCATTAACTCAAAAAATTCTTGAAAGAAAACAAAATGAACAACAAAATCAAAACGATAAAAAAACTCTTGATAAACTAAGATTAGATTTTGCAAACGTCAAAGAAGAATTACAAGCATTGATTAATTCAAAAGATGGAAAATCAGTAGATACCACCAAGGCCACTGAAGTATTAAAAAATACTAAAATAACAAATTCATCTGCTGCCTTTCAAGTTCTTCAAGCAGTTCAAACAATTAAAAAAGCTACAAAAGAATTGCAAGAAGCAATTAAATTTAAGAAGCAACAAGAATTTAATGAATTTAATAAAATAAAGCAAGAATTAACAGATTACATTGCAAACGATTTAAATGATTTAAAATACAAAGATATTAAGGCGAAGGCAGAAAAATCAATTGAAAAAGTTAGCGATATAAACATTAATAGTTCTTTTATTAGCGATATTCAACAAGCAACTAAATTATTAATTGATGCCAAAAAACAAGCAATCTCAGAAGTAGAAAAAATTAATAAAAGTAGTGAATTAGACAAATATAAAATCGCTAAAAACAAATTGGAACTATTAATTAAAACCGAAGATGCTCAAATGATAAAAAGTGCCAACGAAAAAGCTAAAAAGATATTAGCAGAAAATACAACAAAGGATACTACATTATCATCTGAAGAATTTAAGACAAGAAGAATAAATCTTGAAAACGCTCTAGATGAACTTAAAGAAGCAATTAAGAATGAAAAGAATATTCAAAAGCAAGAATTTGAAGCGAAAAAACAAGAATTAAAGACTTTGCTAGAATCAGAATGGTCAAAAGATATTGACAAGAAAAACGAAACAACAGTCTGA", "aa": "MNKKIGILLSISLLATAATTATIAAIAAKGKFKKQQELKQNIEKSKAELNKAKSILEKLLKSDYAKNVNKLEESKILDNAKLNENSSLQEIKNKTKQVKEAINSLTQKILERKQNEQQNQNDKKTLDKLRLDFANVKEELQALINSKDGKSVDTTKATEVLKNTKITNSSAAFQVLQAVQTIKKATKELQEAIKFKKQQEFNEFNKIKQELTDYIANDLNDLKYKDIKAKAEKSIEKVSDININSSFISDIQQATKLLIDAKKQAISEVEKINKSSELDKYKIAKNKLELLIKTEDAQMIKSANEKAKKILAENTTKDTTLSSEEFKTRRINLENALDELKEAIKNEKNIQKQEFEAKKQELKTLLESEWSKDIDKKNETTV", "aa_hexdigest": "088babc4500d04a68871cb235460abcf", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 463213, "stop": 464664, "observations": { "mutation": [ 463518 ], "stop": [ 463518 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019393273", "query_cov": 1.0, "subject_cov": 0.5761689291101055, "identity": 0.885, "score": 556.0, "evalue": 8.63e-193, "gene_start": 1, "gene_end": 382, "reference_start": 1, "reference_end": 382, "reference_sequence": "MNKKIGILLSISLLATAATTATIAAIAAKGKFKKQQELKQNIEKSKAELNKAKSILEKLLNSDYTKNVNKLEESKILDNAKIDENSSLQDIKNKTKQVKEAINSLTQKILERKQKEQQNQNDKKTLDKLRLDFANAQEELKALINSKDGKSVDTTKAAEVLKNTKITNSSAAFQVLQAVQTIKKATKELQEAIKFKKQQEFNEFNKAKQELANYIANDLKDLKYKDIKAKAEKSIEKVSDININSSFISDIQQATKLLIDAKKQAISEVEKINKSSELDKYKIAKNKLELLIKTEDAQMIKSANEKAKKILAENTTKDTTLSSEEFKTRRINLENALDELKEAIKNEKNIQKQEFEAKKQELKTLLESEWSKDIDKKNETAVWNNAKIDDSDSIKEIKEATKEVQGAIDSLIQKMDEKNKLNPLYAKEKFEKIQKQLQDLINSEDAKFIDTNKASETLKNTKITSTSSIDEINQAIKTLKDTIVELKNAINGAKEDSIKEFDKVKKDLEVLVGSEDAKSIEKRTEIGILANTYILERDTIKQIKEKTEAIKKAISSLNSKIENAKALKNELQKFNDIKAALEQLIKTDDAKEVGTEDASKALNNNKVNENSTLEEITNATKALEDAKSKLDQEIKAKKEAEFNNLTNAKTKLSDLITSSSNQA" }, "paralog": false, "identity": 0.9006211180124224, "score": 790.0, "evalue": 4.42e-287, "description": "Internal stop codon at 463518. Nonsense mutation around 463518.", "psc": { "uniref90_id": "UniRef90_UPI0019393273", "uniref50_id": "UniRef50_UPI0019393273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019393273", "UniRef:UniRef50_UPI0019393273" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0019393273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019393273" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 43433.30390000001, "isoelectric_point": 9.390545463562013 }, "id": "DFOKDJOJIG_827", "locus": "GCA013367855_00817" }, { "type": "cds", "sequence": "contig_1", "start": 465030, "stop": 465350, "strand": "-", "frame": 2, "gene": "hinT", "product": "histidine triad protein HinT", "db_xrefs": [ "COG:COG0537", "COG:FR", "RefSeq:WP_175972300.1", "SO:0001217", "UniParc:UPI00000BD087", "UniRef:UniRef100_Q9JN38", "UniRef:UniRef50_P32083", "UniRef:UniRef90_D1J8D6" ], "nt": "ATGCATGATGATATTTTTGAAAAAATAATAACAAGAGAAATCCCGGCAAAAATAATTTATGAAGATGAAAAATTTATTGCCTTTGAAGATATTAAACCAGTAGATAAAGGCCATTTTTTAGTAACAGTAAAGAACCACGCCACAAATTTATATGATATTGATGACCAAACATTGTCATCACTAATATTAAAGTCTAGAGAACTTGCTTTAAAAAGAGTTAAGGAATTAGGTGCAACTGGATTTAATTTAATAATAAATAATAATCTTTCAGCAGATCAAACAGTATTCAGAATTCATGTTCATATAATCCCAAGATATTAA", "aa": "MHDDIFEKIITREIPAKIIYEDEKFIAFEDIKPVDKGHFLVTVKNHATNLYDIDDQTLSSLILKSRELALKRVKELGATGFNLIINNNLSADQTVFRIHVHIIPRY", "aa_hexdigest": "07713d17a6d027080de203322ed59070", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000BD087", "ncbi_nrp_id": "WP_175972300.1", "uniref100_id": "UniRef100_Q9JN38", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BD087", "RefSeq:WP_175972300.1", "UniRef:UniRef100_Q9JN38" ] }, "ips": { "uniref100_id": "UniRef100_Q9JN38", "uniref90_id": "UniRef90_D1J8D6" }, "psc": { "uniref90_id": "UniRef90_D1J8D6", "gene": "hinT", "product": "histidine triad protein HinT", "uniref50_id": "UniRef50_P32083", "cog_id": "COG0537", "cog_category": "FR" }, "pscc": { "uniref50_id": "UniRef50_P32083", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P32083" ], "product": "Uncharacterized 13.1 kDa HIT-like protein in P37 5'region" }, "genes": [ "hinT" ], "id": "DFOKDJOJIG_828", "locus": "GCA013367855_00818" }, { "type": "cds", "sequence": "contig_1", "start": 465352, "stop": 465480, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTGCTAATTTTGAGTCATTATATAAAACTGCAGTTAAATATATTAATGAACTAGGGTTTAAATTAAATGTTTCAGAAACAAACAAATCTGTTTATGATATATTGAAAGTTGAAGGTCTAGTATAA", "aa": "MTANFESLYKTAVKYINELGFKLNVSETNKSVYDILKVEGLV", "aa_hexdigest": "bbfeebaf8b194e99c537736287b7ff2e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4770.4540000000015, "isoelectric_point": 6.0701997756958 }, "id": "DFOKDJOJIG_829", "locus": "GCA013367855_00819" }, { "type": "cds", "sequence": "contig_1", "start": 465520, "stop": 466215, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGGCGAAGTATTAAAATCATTATTGGCATATAAAGGCATAGTTAAAAATTCAAATACGTCAGGTGATTTAGGTGGCAATTTAGATAGTATAAAGAAAGATTTAAGTTCAGTATATGGATTTGTAGATCCATATAGCAAAAAAGTTTATAGTCAAGAAAGCTTTTTACTTGCAAAAATTCTTGCCCAAGAAATAAATCATCCAAAGGCAAAAGCAACCGAAACATTGCAATCTAAAGTTTCAAAGGGTGAACTAAAATCGTTTGATTACAAAGATTACGAATTTGAAGGTTTAACAAAAGATAGCAAGGATAATTATCAATATACAAAGACAATAACTCTTGATAACAAGCAATATACATTGAGATTTAGCCAAAAAGGTTCAATATCATTTGATGGAAACTTTTTAACAATTCCTATGAATTTAACAGTTGATGGTTTAGGAAAAAGAAATTTCTATGAATTCAATGCTAAACTAGAATACAATAAAAATACAAAGAAATTTGAGTCAATAAAACAAGATGTTGCATACAATTTAAAACAAAATCCTCAAAGAATAAATGTTACAAAAGACAATTCAATTACTGCCCAATATGTAGTTAAAATTTCACCATTATATTTAACTAAGAAAGTTAAAGATTATAACGGCAAAGAAGTTACAAAACAAGTTTTAACATTTGATGAAACTCCATGA", "aa": "MEGEVLKSLLAYKGIVKNSNTSGDLGGNLDSIKKDLSSVYGFVDPYSKKVYSQESFLLAKILAQEINHPKAKATETLQSKVSKGELKSFDYKDYEFEGLTKDSKDNYQYTKTITLDNKQYTLRFSQKGSISFDGNFLTIPMNLTVDGLGKRNFYEFNAKLEYNKNTKKFESIKQDVAYNLKQNPQRINVTKDNSITAQYVVKISPLYLTKKVKDYNGKEVTKQVLTFDETP", "aa_hexdigest": "7aeff925e648f2aaea1e654071a61833", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26324.50890000002, "isoelectric_point": 9.232404518127442 }, "id": "DFOKDJOJIG_830", "locus": "GCA013367855_00820" }, { "type": "cds", "sequence": "contig_1", "start": 466378, "stop": 466734, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATCAATGCAAAGATTGACACAACTAACTATAATCCTGCTGCATTTTCAATACCAAATGAACAAGAATTTAAATTTATTTTTAAACACTCAAATCAAATCGCGGCAAACATTAGATTAGAACTTCAAAAAATGTTACTTGCTAAACTTTATTTATTAAAAAATAGACCAGAACTTAAAAAAATAGCAAATGATAGCAATGGTCTAGACAAAGCACAAGTTGCATTACACAACAAAATGTCTAAAAAAGATGCTCCTATTAATGAAAAAGAATTATACGAAGCATTGAATTTCGCAGATGATTCATTGTATTTAATGAAATATCTAGTTGAAAATCCGATTATTGAAAATTGA", "aa": "MFINAKIDTTNYNPAAFSIPNEQEFKFIFKHSNQIAANIRLELQKMLLAKLYLLKNRPELKKIANDSNGLDKAQVALHNKMSKKDAPINEKELYEALNFADDSLYLMKYLVENPIIEN", "aa_hexdigest": "a47dd4e8121b00def68312f73452e280", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13645.632299999981, "isoelectric_point": 7.883854866027832 }, "id": "DFOKDJOJIG_831", "locus": "GCA013367855_00821" }, { "type": "cds", "sequence": "contig_1", "start": 466936, "stop": 467100, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAAAAAAATTAGATTATTAACAGCTATTGCAATGCCACTTGCGGTTCTTCCTTTGATTGCTAGTTCATGTAAAAAAGAAAAGGAAGATTCTCAACAAAATTCAGGGTACCAAAAACGTGTTTTAAAAGATTCATTAACAAAAAACAGGGTTTTAACTTGA", "aa": "MTKKIRLLTAIAMPLAVLPLIASSCKKEKEDSQQNSGYQKRVLKDSLTKNRVLT", "aa_hexdigest": "96c489145654e415ec9ec61e1a5af3c0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6059.176500000003, "isoelectric_point": 10.103952598571777 }, "id": "DFOKDJOJIG_832", "locus": "GCA013367855_00822" }, { "type": "cds", "sequence": "contig_1", "start": 467112, "stop": 468236, "strand": "-", "frame": 2, "gene": null, "product": "HinT-interacting membrane complex protein P80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BDBC09" ], "nt": "TTGAATGAAAGTGATGACAAGGTTTCAATTAAACAAGGCATTGATGCCATTTCAACATTTAAAGGTATATCTTCACTTCTTAAAACAAATGAAATTACTAAAGAGCAAGAAATTGCAGCCAAAAATGATAGCAACCTTTTGAGATATGTTTCAAGTGATAGCAAGTCAGCTGATAAATACGGTTTCGATGGCTTTAAAAGTGTGTTGAATAGTTTAAAAGAAAAAGATCCTAATAAGTATATGAATCTTCTTTCAATCTTAATGGGGGATGCTGACAAAGAAAAAAGCATTTTTAAAGTTGAAGATAAAGATGATTTGTTTACAAAATTAAAAGAAAACTTCAAAAAGATCTTTGGCGATGCTACATTTATTGATCATGAAAAGAAAACAAAGAAATTTGCCGATATTTTTAATGAAGGAACTGAAAGTGTTGACAAAGAAAACTTCTCTGAAAAATACACTAGATACAATGCAGCTATTGAAAAATTTATCAATGATTTAAGTGAAAAAGATTTTAATAAATCTTTTGGTTTGGCATTTCGAGATGCATTTAGTGATGAAGCAAGTGGGTATAAGGTTTCAACATTAATTAAAAGTAATGGAAATTACATTCAAGTCAATGAAAGTGGCATTTTGATTCAAAATGTATTTTCATTAGACAAAGAAGAAGTTGTAACTAGATTGATAACAAATGATTTGTCAATACTATCTAAGGCCAACCACTCAGATACATTAAATACCAAATTGTTTAATTTAGATTCAATATTTTCTGAAATTTTAACTCGTGACTATATGGTTAATGATTTATTACAACAAGATAATTTTAAAAATTATATTAAATCAAAAGAATATTTAAGTCTTGAAGGAACAAAACGTAAATTCAATGATCAAGATATTTTAAATGCAATCAACTATGAAAAATTAATTCGCGAAACAAGTAAATACGTTTTAATTAATGGTAAAGCAAACGAAATAAAACAATTTATAATTAAAAAATTTAATAGTAATTTATACAAAGATTATGTGTTCAAGAACAATAAGTTTATTCTATCACCACATGAAGACAAAGAGATCTTATCTTATTTATTCACAACAATTGTAAATTACATAAATACTAAAAATTAA", "aa": "MNESDDKVSIKQGIDAISTFKGISSLLKTNEITKEQEIAAKNDSNLLRYVSSDSKSADKYGFDGFKSVLNSLKEKDPNKYMNLLSILMGDADKEKSIFKVEDKDDLFTKLKENFKKIFGDATFIDHEKKTKKFADIFNEGTESVDKENFSEKYTRYNAAIEKFINDLSEKDFNKSFGLAFRDAFSDEASGYKVSTLIKSNGNYIQVNESGILIQNVFSLDKEEVVTRLITNDLSILSKANHSDTLNTKLFNLDSIFSEILTRDYMVNDLLQQDNFKNYIKSKEYLSLEGTKRKFNDQDILNAINYEKLIRETSKYVLINGKANEIKQFIIKKFNSNLYKDYVFKNNKFILSPHEDKEILSYLFTTIVNYINTKN", "aa_hexdigest": "5c08c13063a796c3232154788f546a71", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BDBC09", "query_cov": 0.8155080213903744, "subject_cov": 0.9967320261437909, "identity": 0.531, "score": 296.0, "evalue": 1.07e-95, "valid": false, "product": "HinT-interacting membrane complex protein P80", "uniref50_id": "UniRef50_UPI0021BDBC09" }, "pscc": { "uniref50_id": "UniRef50_UPI0021BDBC09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BDBC09" ], "product": "HinT-interacting membrane complex protein P80" }, "genes": [], "id": "DFOKDJOJIG_833", "locus": "GCA013367855_00823" }, { "type": "cds", "sequence": "contig_1", "start": 468912, "stop": 469034, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACTATGGTGATATTAATAAAATCCATGAAATAGCAAATTCATCAAAACATGCTGCTGAAGGTGTTGAAAAGCAACTAATTAAATACCTTTATGAAAAAGAATATGAAGGTTCATTGTGA", "aa": "MNYGDINKIHEIANSSKHAAEGVEKQLIKYLYEKEYEGSL", "aa_hexdigest": "fc4f3f946ea3a21e1f35b177f91b4cf9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4614.1062999999995, "isoelectric_point": 5.503799247741698 }, "id": "DFOKDJOJIG_834", "locus": "GCA013367855_00824" }, { "type": "cds", "sequence": "contig_1", "start": 469354, "stop": 469767, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2617A5", "UniRef:UniRef90_UPI001F2617A5" ], "nt": "ATGAATAAATTACTTTTAAATAAAGATAGAATATTGCTTGAATTTATTGAAATTTGTAAAAATCTTAATCTTTTTTATAGTTTAGATAAATTAAGCTTGTATGGCACTATGTCAAAAGAACATTCTTCAAATAATTTAGATCACCATGAAATAATGATGAAATATCAAGATTATGAAATCTTAAAAAATGCTTGTCCTAATAGAGTTTTAGATAATACATTGCATTCTTTGTATCACGACTTACAAATAAAATTTGTTGATGATAACAAAAACATATTTGAAAACCAACCGTTTTTGAACATTAACTTAATTTATCCAACAAACATTGCCAATATAAAGAAATATTTAAAACATTGCAATCAAGCTAGATCATTTATTGGCCACTATTTAACAATGACAAATAGCAACGATTGA", "aa": "MNKLLLNKDRILLEFIEICKNLNLFYSLDKLSLYGTMSKEHSSNNLDHHEIMMKYQDYEILKNACPNRVLDNTLHSLYHDLQIKFVDDNKNIFENQPFLNINLIYPTNIANIKKYLKHCNQARSFIGHYLTMTNSND", "aa_hexdigest": "9b9385b6d21ae8f786ad16ca1c1fef60", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 469354, "stop": 469879, "observations": { "insertion": [ 469855 ], "mutation": [ 469765 ], "stop": [ 469765 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2617A5", "query_cov": 1.0, "subject_cov": 0.7873563218390804, "identity": 0.985, "score": 274.0, "evalue": 7.72e-93, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MNKLLLNKDRILLEFIEICKNLNLFYSLDKLSLYGTMSKEHSWNNLDHHEIMMKYQDYEILKNACPNRVLDNTLHSLYHDLQIKFVDDNKNIFENQPFLNINLIYPTSIANIKKYLKHCNQARSFIGHYLTMTNSNDWKTKNNIIKSHFLSMFYKPITYKEVANLLIKMITKVF" }, "paralog": false, "identity": 0.9770114942528736, "score": 342.0, "evalue": 4.02e-122, "description": "Internal stop codon at 469765. Frameshift due to insertion around 469855. Nonsense mutation around 469765.", "psc": { "uniref90_id": "UniRef90_UPI001F2617A5", "uniref50_id": "UniRef50_UPI001F2617A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2617A5", "UniRef:UniRef50_UPI001F2617A5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2617A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2617A5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16269.513999999977, "isoelectric_point": 7.045727348327636 }, "id": "DFOKDJOJIG_835", "locus": "GCA013367855_00825" }, { "type": "cds", "sequence": "contig_1", "start": 469921, "stop": 470100, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F176BBD", "UniRef:UniRef90_UPI001F176BBD" ], "nt": "TTGCCAAATATTAAATTTGAAACAAAAAAAGTAATTATAAACAATATTGAAGTTGATGTTTTAGTTGAATTTGACCAATATCTAAAAAACGTTTATGAAGTTGAATATTTTGAACATTCCGTTCCACAAAGAAAGATAGAATATATTAACCCCATTGAATATAAAAAAATATTTAGCTAA", "aa": "MPNIKFETKKVIINNIEVDVLVEFDQYLKNVYEVEYFEHSVPQRKIEYINPIEYKKIFS", "aa_hexdigest": "84b31e13c5316cb7c8a24db57c47004b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F176BBD", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.966, "score": 115.0, "evalue": 8e-33, "valid": true, "uniref50_id": "UniRef50_UPI001F176BBD" }, "pscc": { "uniref50_id": "UniRef50_UPI001F176BBD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F176BBD" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7226.258899999999, "isoelectric_point": 5.270873069763183 }, "id": "DFOKDJOJIG_836", "locus": "GCA013367855_00826" }, { "type": "cds", "sequence": "contig_1", "start": 470261, "stop": 471205, "strand": "-", "frame": 3, "gene": "lolD", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1136", "COG:M", "SO:0001217", "UniRef:UniRef50_A0A448ZXQ2", "UniRef:UniRef90_D1J8E0" ], "nt": "ATGGCAAAAGAAATAAAAGAAAACAAAAAAGAAATTAAAGCTAAAACAAATAAAATTTCTAAGAATAAAAATTTTGTTGATGAACAAACATTGCAAGATTTAAACTTGATAAATATTTCTAAGCAAAAAAAGCCATTTGAAATTAAAGATGAAGGCATTGCAATTAAGGTTTTAGATAATGCAACTTTAAAGAAATATAGACTTGCTAATGACAAACCAAGAAAAAAAGACGAGTATGCTAAATTACAAAATTCTAACGATAACATTGTTGAAGTTGATAATATTAAAAAAACATATTTATCCGGCAATGTAGCAACTGAAGTTTTAAAAGGAATAACTTTTTCAATTAAAAAAGGAGAAATTGTTGTTCTATATGGTAAGTCTGGGTCAGGTAAATCAACATTATTAAACATAATTAGTGCTCTTGATAGGCCTACCTCTGGCAATGTAATTGTAAATAACATAAATTTACCTTATTTATCTGATGCAAAGCAAACCTTATTTAGAAGAGAAAACATTTCGTTCATTTTTCAAAATTATAATTTACTTCAAAATTTGAATAGTTATGACAATGTTGAAACTGGTTCATATTTACAAAGAGATAAAAACAAGCGTTTAAATATTAAAAAATTATTTGAAGATTTTGATCTTTCTGAATGTATGTATAAATATCCTTCTCAAATGTCTGGTGGTCAACAACAAAGAGTGTCAATTTTAAGAGCCATTGCAAAAAATTCCGAAATTCTTGTAGCTGATGAACCAACAGGAGCATTGGACGAAAAAACAGGAATTATTGTATTGAAAAATTTGCAACAAATCAATTTAAAATATGGCACAACCATTATCATTGTTTCCCACGACCCTGATGTTGCCCAAATTGCTGATAAAGTTATATACTTGGAGTTGGGATTAATTAAAGAAGTAAAACTTCAAGAAAGAAAATGA", "aa": "MAKEIKENKKEIKAKTNKISKNKNFVDEQTLQDLNLINISKQKKPFEIKDEGIAIKVLDNATLKKYRLANDKPRKKDEYAKLQNSNDNIVEVDNIKKTYLSGNVATEVLKGITFSIKKGEIVVLYGKSGSGKSTLLNIISALDRPTSGNVIVNNINLPYLSDAKQTLFRRENISFIFQNYNLLQNLNSYDNVETGSYLQRDKNKRLNIKKLFEDFDLSECMYKYPSQMSGGQQQRVSILRAIAKNSEILVADEPTGALDEKTGIIVLKNLQQINLKYGTTIIIVSHDPDVAQIADKVIYLELGLIKEVKLQERK", "aa_hexdigest": "662dd1bababf85e6d39c7f1fafb15a6c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8E0", "query_cov": 1.0, "subject_cov": 0.9721362229102167, "identity": 0.997, "score": 587.0, "evalue": 5.27e-211, "valid": true, "gene": "LolD", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A448ZXQ2", "cog_id": "COG1136", "cog_category": "M" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ2" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "lolD" ], "id": "DFOKDJOJIG_837", "locus": "GCA013367855_00827" }, { "type": "cds", "sequence": "contig_1", "start": 471255, "stop": 471935, "strand": "-", "frame": 2, "gene": null, "product": "FtsX-like permease family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16D702", "UniRef:UniRef90_UPI001F16D702" ], "nt": "ATGTTTATAAGCAATCCAAAATTAAAATATATTTCTGTATATGAACATAATATTCAATCATATAATGAAGCACATCCTCAGGCAAAATTAGATTATAAAACTCAATTAAAGAAATTACTTGGAAATCTAAACGATGAAAGTATACAAGAAATAATAGACTCAGATAGCCCATTAGAAAGTTATAAAATCTTAGTAAGAGAAACATTAACCAAATTCTATGGCACACAAGCATCTTCAATATATGGCAAGAATATAATGTATGGTGCATCATTTGATGTTAATAGTAAAGACATTGAAGCCGGATTTATTGAAGGAATTTCTTCAACAGTTAATACCATATTGCTGGCATTTATAATAATTTCATTGATAATTTCAATAGTTATATTAATTGTTATTACTAATATAATGATTGCTTCTAATGGAAGATCAATTGCTACATTTTCTATATTGGGATATACAAATAAAGAAAAAATTCTATTATTCTTCTTCAATTTTGTACCTGCAATTGTATTTGCTTGCTTACTAATGATTCCTGCAACTCTTGCAATAATAGCAATATTCAACTCGTTTATGATGGTAACTAGCCAGATTGTATTACCTTTGGTTTTACATATTTCAACAGTAATATTAAGTGCATTAATTTGTTTGGCAGTATTTATAGCAACATCAATTGCAACATGA", "aa": "MFISNPKLKYISVYEHNIQSYNEAHPQAKLDYKTQLKKLLGNLNDESIQEIIDSDSPLESYKILVRETLTKFYGTQASSIYGKNIMYGASFDVNSKDIEAGFIEGISSTVNTILLAFIIISLIISIVILIVITNIMIASNGRSIATFSILGYTNKEKILLFFFNFVPAIVFACLLMIPATLAIIAIFNSFMMVTSQIVLPLVLHISTVILSALICLAVFIATSIAT", "aa_hexdigest": "eca63e3e956efbfb1658aec33baa0952", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 471207, "stop": 472070, "observations": { "mutation": [ 471257, 471953, 472004 ], "stop": [ 471257, 471953, 472004 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F16D702", "query_cov": 1.0, "subject_cov": 0.7874564459930313, "identity": 0.996, "score": 385.0, "evalue": 1.95e-133, "gene_start": 1, "gene_end": 226, "reference_start": 46, "reference_end": 271, "reference_sequence": "MSIDKVPVQTIDTLTTYSATGFWGAASSYDVAEANDNSAWDFFKRMFISNPKLKYISVYEHNIQSYNEAHPQAKLDYKTQLKKLLGNLNDENIQEIIDSDSPLESYKILVRETLTKFYGTQASSIYGKNIMYGASFDVNSKDIEAGFIEGISSTVNTILLAFIIISLIISIVILIVITNIMIASNGRSIATFSILGYTNKEKILLFFFNFVPAIVFACLLMIPATLAIIAIFNSFMMVTSQIVLPLVLHISTVILSALICLAVFIATSIATWNSLNKIKAVDALKGK" }, "paralog": false, "identity": 0.9825783972125436, "score": 513.0, "evalue": 1.92e-186, "description": "Internal stop codon at 471257, 471953, 472004. Nonsense mutation around 471257, 471953, 472004.", "psc": { "uniref90_id": "UniRef90_UPI001F16D702", "product": "FtsX-like permease family protein", "uniref50_id": "UniRef50_UPI001F16D702", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F16D702", "UniRef:UniRef50_UPI001F16D702" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F16D702", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16D702" ], "product": "FtsX-like permease family protein" } }, "id": "DFOKDJOJIG_838", "locus": "GCA013367855_00828" }, { "type": "cds", "sequence": "contig_1", "start": 472002, "stop": 472505, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTGCAAGATAGTCGGGGCAATAATTTAACAAAATTATTACAAGACTATAATTACAATTTTGATAGTAATGATCCAATTCCTGTTGTTGTAAATGAAGTAACGGCAAGAAGCTATAGATTAAAAGTTAATTCCGTATTTGATGCCAAAATATTAAATCACGTTGATCGATTTGCATATCAAGCTTTAATGCAAAAAAGCCCAAGAGTCGATTATAAGTTTAAAGTAATTGGCATATCAGATACTTATATTAACTCCGAAATTAGTACAAGGAAAGATATTTTAGACCACATTTTAGGTTATGATACACTTTCAAAAAGATTGAAAGATGCAAGAAAATACGAATTACAAAAAGCAATTAGTTTACACCCTGATTTAAAAGATGAATATATTAAAAAATTCAATAAGAAATATGATGCATTTAACGGTATTTTGTCAATTGATAAAGTTCCAGTTCAAACTATTGATACATTAACAACTTATTCAGCAACAGGATTCTGA", "aa": "MRLQDSRGNNLTKLLQDYNYNFDSNDPIPVVVNEVTARSYRLKVNSVFDAKILNHVDRFAYQALMQKSPRVDYKFKVIGISDTYINSEISTRKDILDHILGYDTLSKRLKDARKYELQKAISLHPDLKDEYIKKFNKKYDAFNGILSIDKVPVQTIDTLTTYSATGF", "aa_hexdigest": "02184476eb8b040f50b5b243381ec2ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19365.8267, "isoelectric_point": 9.220929145812985 }, "id": "DFOKDJOJIG_839", "locus": "GCA013367855_00829" }, { "type": "cds", "sequence": "contig_1", "start": 472740, "stop": 473048, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGAAACACAAAGGGCAAGAGTAAACCAAATATTTAACCGCGTAAGTTCAATGATGCAATCAACTCAAAACTTAATAAATTTACGTAGATTAAAAAATGGTGGAGATTATAGAATAATAGATGAAGAAGATGTAAAAGATCCAACAAAAATAATTGCTGTAAAAGATATTAAAAAATTCTTTGATGACTATAATGCTAAACGTCCAGAAGATTTATCAAATAGAACAAGTTATTTCTTGTTTAGCGCTGGTAATTATTCAGAAGCAGCATCAGGAAATAGAAAACAATTTAGATTTGTTGAATGA", "aa": "MPETQRARVNQIFNRVSSMMQSTQNLINLRRLKNGGDYRIIDEEDVKDPTKIIAVKDIKKFFDDYNAKRPEDLSNRTSYFLFSAGNYSEAASGNRKQFRFVE", "aa_hexdigest": "6b08e1a730bc0aa42ee5b39d2731b77b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11945.299099999987, "isoelectric_point": 9.51716136932373 }, "id": "DFOKDJOJIG_840", "locus": "GCA013367855_00830" }, { "type": "cds", "sequence": "contig_1", "start": 473067, "stop": 473453, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTCGTTATTCTTAGCATTTTCAACGACTTCATTAATTTCTATGTTCTTCTTATCTTCAAATAACGTATTTAATAAAACTATTTCAAAGACATATAAAAATAGGCTATATAAATATAAATTAAATCTTGAAAGTCCAACAACCGAAGGTGGTCCTTATGTTACATACAATCAAAACGATATAAATGATTTATTGTATGTTCCTAATGATCTTGCAGGTGATTCATCAACAAATGGTAGTCAACTAGATTATGATAATCCTAATTTCTTAAGACCTGGAGCTTCATTTAATACAGATGTAATTCAAAGAAAATTTGCGCCTACTGTCATTACAAAATCATCGCTTGATTTATTAATGGATTTATCAGTTGAATTAAGTCCATGA", "aa": "MFSLFLAFSTTSLISMFFLSSNNVFNKTISKTYKNRLYKYKLNLESPTTEGGPYVTYNQNDINDLLYVPNDLAGDSSTNGSQLDYDNPNFLRPGASFNTDVIQRKFAPTVITKSSLDLLMDLSVELSP", "aa_hexdigest": "81bfa1e11e916d49b86f73582905571f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14374.00049999998, "isoelectric_point": 4.882492256164551 }, "id": "DFOKDJOJIG_841", "locus": "GCA013367855_00831" }, { "type": "cds", "sequence": "contig_1", "start": 473457, "stop": 473642, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTTGTTTCACTAGTTATTTATATAATTACTAGAGTTATTGTAAGAAATAAACCTACAGAATTAATGAGTGGGTTAACTGAAGTTAGTGTTGGTAATTTTGCCCAAAGAGTTTCTGCTATGTTTAGAAAAATGCCAGTTAAATCAAGATTTATTGCCTCGATGGCATTGAATAATTTCTGA", "aa": "MIVVSLVIYIITRVIVRNKPTELMSGLTEVSVGNFAQRVSAMFRKMPVKSRFIASMALNNF", "aa_hexdigest": "b8c80b17c836aff5f20138501a276960", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6876.251800000002, "isoelectric_point": 11.466427421569826 }, "id": "DFOKDJOJIG_842", "locus": "GCA013367855_00832" }, { "type": "cds", "sequence": "contig_1", "start": 473793, "stop": 475259, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTTGCTCACGCACATACAGCAAAAGCAACAAAGCAAATTATGGATTTAATGAATAATTCAGCAGTATTTAAAAATTTATTTGACCAATTATCTAAAAATCATGTTTCTAATAAGGGCATTGAAGCAATTAAAGAGATATTAAAAAATAATATGAATGAAATAACCTACAATTTTGGTTATATGGCCTCTTCAAATCAAATGCCAACTCATTATCTTCAAGCCTTAAAATTATTTTTACAATCATTTATTAATGTCAATGATCAAAATCAAAAAATTAAACCGTTAATAAATGATGAATATACCTTTAGCTTAGTATATAAGGATGCAATTGCAGCTTCAAAAACCGCCTCAATAATTTCGTTGTTAAACGTGCCAAGAAATATTCTAAATGGTTTCAATATTTTAAGTTTCCCACAAATATTGTTATACTACGTATTAAGTCCAAACCCAGATAGTGGAAATATTGCTTATATAATCAAAAAATTATTATCGAATATTGATGAAACTAATGCAACAATGTTGAAAAAACAAATCGAAGGTGCAACTCAAAAATTCAATTTTGTTGAAAATGTTGTTACTTCAAAAAGCGATAGCAGAATTGATTTAGATATTTCTAAATTCAATTATATGTTTAACCATTTACTAAAAACACCCGATAACAAAGATATTAGCTTTTTTGGAATAAATATTACCAAAACTGTTAAGGAAGTATTTTCAAAAATAATAGAACCAAGCACAGTTTATAATTTAATTGCATATTCCGAAGCTGCTTCATATTTAGCCAAGGTAAATTATGGATATTTAAACAAAAATAAAAAAGAAGTTTACAATGGCGATATTTCGAAATATTTAAATAATCCATTTGAAATGCAACAATTCATTGCTTCATTAGATGATAAATATAAGATTAAAGTAAATACTCAAGAATATTTGATAACAGGTATTGATAGCACCGCTGATTATTTATATCCTGTAATTAATGAAGAAAACTTACAAGTTGATACAAAAACTCAATCTGTTGTTTATGTTAATTCAAGAGGCTTTGACAGAATTAGATCGGCATATCCTACATTTGCCTTAAAGAGTTATGCATTAGTAAAATCTCCTGTTGACAAAAAAGGAAAATATTTAGCTGGCAAAGATCCTAAAAACTTAAAGAAAGAACTTCAAAGAATTGTTTCATCAATTTCTCCAAATTCAAAAAATAAAGTATTTTTAAAAGATGAACCAGATGCAATCAACCCCGAACGTTACATCAGAGTAAAAACAATAAGAACATTAGTAACATCAATTAAAAATGCAACACTTTATTTAATAACAATTTTGGCAATATTAGTAATTTTGATTGTTTACTTTATTATCAAAAGATATATTGAAGCAAGAAATAAGGTTGTTGGAATATTAAGAGCTCAAGGATATAAAACATCAGAAATTGCTTTATCATTCTGTGCTTTTGGCTGA", "aa": "MPFAHAHTAKATKQIMDLMNNSAVFKNLFDQLSKNHVSNKGIEAIKEILKNNMNEITYNFGYMASSNQMPTHYLQALKLFLQSFINVNDQNQKIKPLINDEYTFSLVYKDAIAASKTASIISLLNVPRNILNGFNILSFPQILLYYVLSPNPDSGNIAYIIKKLLSNIDETNATMLKKQIEGATQKFNFVENVVTSKSDSRIDLDISKFNYMFNHLLKTPDNKDISFFGINITKTVKEVFSKIIEPSTVYNLIAYSEAASYLAKVNYGYLNKNKKEVYNGDISKYLNNPFEMQQFIASLDDKYKIKVNTQEYLITGIDSTADYLYPVINEENLQVDTKTQSVVYVNSRGFDRIRSAYPTFALKSYALVKSPVDKKGKYLAGKDPKNLKKELQRIVSSISPNSKNKVFLKDEPDAINPERYIRVKTIRTLVTSIKNATLYLITILAILVILIVYFIIKRYIEARNKVVGILRAQGYKTSEIALSFCAFG", "aa_hexdigest": "a92b6fea75b9bb406c77d71edc57f6d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 55519.52119999996, "isoelectric_point": 9.445730400085449 }, "id": "DFOKDJOJIG_843", "locus": "GCA013367855_00833" }, { "type": "cds", "sequence": "contig_1", "start": 475527, "stop": 476321, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGCAACTAGTTAAACATGAACATTATCCAATTGTTGCAAATAATAAAATTTATAAAGATTTCAACACAGAAAGTTTAACTAGTTCAGATTATTTAACATCATTTTTTGGTGCAATAAGTTCAAACCTAGATGGAAAAATAAATAGCGGAAAAATTTCCCAAATTCAAGAATCCGTTATTCAAATGTTTAATTTTTCAAATAAGACAAAGAGAATTTTAGGTAAATTGAATATAACTATTCCTGATTATGACAATAATAAAATTACTTTGTTTGATTTAATAAACATGTCAAAATTTTCATTCCCTCAATCTCAAAAGCAAACAAATGTATTGACTAATATTCCAATAAATCCTTTTGATGTTGATGATATTAAAAAAATATTATTAAAGGCAAAAGATGCAAAAATAAATAACAAAAAGTTGAAATTAACTCAAAACGAATTTGATGTATTAAAAAACAATGTTTTAGTTACCAAAGACGAATTTAATGACCTTGATTTAATAATTTCAAAATTAACTAAATATCATGAATATATAGACAAACTTGTAACATCAAACTCTCAATATAAGCAAAGCGGTATAAATTCAGCAGTTGAATTAAATGATTTAAAATCTTATTTGGATGTTGCATATTATTCGGCTTCATTTAATAATGTAGCGCCTGAACATGCAAAAGATTTGGAAATTTTAAAAACTATTCATTCATTAATAAAAGACAATTTAGCAAAATTAATGTTGAATAATGATCCACAAAATGTTGCTTCTAATGTCTTATCTCTATATTCATTATGA", "aa": "MEQLVKHEHYPIVANNKIYKDFNTESLTSSDYLTSFFGAISSNLDGKINSGKISQIQESVIQMFNFSNKTKRILGKLNITIPDYDNNKITLFDLINMSKFSFPQSQKQTNVLTNIPINPFDVDDIKKILLKAKDAKINNKKLKLTQNEFDVLKNNVLVTKDEFNDLDLIISKLTKYHEYIDKLVTSNSQYKQSGINSAVELNDLKSYLDVAYYSASFNNVAPEHAKDLEILKTIHSLIKDNLAKLMLNNDPQNVASNVLSLYSL", "aa_hexdigest": "1e856747bc3bf87b6251ca5ccea51343", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30065.933500000043, "isoelectric_point": 7.763774299621583 }, "id": "DFOKDJOJIG_844", "locus": "GCA013367855_00834" }, { "type": "cds", "sequence": "contig_1", "start": 476694, "stop": 477299, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTATAAGTTTCGGGGCAATAAACAACAATTTAACCAACGAAATTATTCAAAATAAAACAATAAATGGTTTAGTTAATGTTATTAGAGATGCTTTATTCAAAAGCGAAATTATTAACATGTTCAATAAAAATGATTTATTGAGAATCATGGATGCAGCAAAACAAAGTGTTGTGGATAACGATTTCCATACATTGCTAAATATTGGCAAGGTAAATGGCACGATTTTACAAAAAGTTCTTTTAGATATTTTGAAATACATTATTAGACCAGTAGATAGAACTAATAGTAACAATTTAAATATTAATAATGTTAATGTTAATGCTTTTGTTCAAAATGTATTCATTGGAATAATAAACTATTTAGAAAAAATGTATATGAATTCAGGAACCAATGAAAATGAACGAAATCAATATTTAATGCAACAAGTTTACAATATAAGTAAATTATTAAATCTTGATAATCTATATTTGATTCCTTCTTTGAAATTAAGTTTATTTGACATAATTAATTTAGTTAAAAATAAATCAACAATTTTTGAAGTATTGAGAAATGTTATTCGCTCTGTTGATTTTGAAAAATTTTCTTTAATTATTCAAGATTGA", "aa": "MPISFGAINNNLTNEIIQNKTINGLVNVIRDALFKSEIINMFNKNDLLRIMDAAKQSVVDNDFHTLLNIGKVNGTILQKVLLDILKYIIRPVDRTNSNNLNINNVNVNAFVQNVFIGIINYLEKMYMNSGTNENERNQYLMQQVYNISKLLNLDNLYLIPSLKLSLFDIINLVKNKSTIFEVLRNVIRSVDFEKFSLIIQD", "aa_hexdigest": "a6c2b8ff88f3d0333115b5bbd4d2a0a2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23124.58050000002, "isoelectric_point": 8.751921653747559 }, "id": "DFOKDJOJIG_845", "locus": "GCA013367855_00835" }, { "type": "cds", "sequence": "contig_1", "start": 477444, "stop": 477578, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACCGTTGCTAATGATGAACCAAAGACCGTTGGCTCTTCAATAGTTGGTGCAGTTGCAAGAATTAGTGATGAAAAATACATTTTATTAAAATATTCAACTATTGATGGATTTAGTGATAAAAAAGTTTGA", "aa": "MLTVANDEPKTVGSSIVGAVARISDEKYILLKYSTIDGFSDKKV", "aa_hexdigest": "236ba6bff1dffd2e49b11d2a932d34fe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4760.418100000001, "isoelectric_point": 6.0263769149780275 }, "id": "DFOKDJOJIG_846", "locus": "GCA013367855_00836" }, { "type": "cds", "sequence": "contig_1", "start": 478503, "stop": 478784, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACACTATTGATGGTAGAGATGTAATGAAGATGCTTGGTTTTAAAGAAATTTCAGAAAACCATTATGAATTTGATGCATCTCTTTATAATAAAAACTCTGATTTAAAAATCGAAAATTCGGATTTTACAAGTCAAAAATCAAAATTTATTAATGAAGACTTTAATATTTCAAGAACTTTTAAATTTCAAAATTTTTTAAGCAATAAAAGTATTGAAAAAGATGTTAATGAATTTACAAAACTAGCAGCAAATAAAATATATCAAATTCCAAATGAATGA", "aa": "MHTIDGRDVMKMLGFKEISENHYEFDASLYNKNSDLKIENSDFTSQKSKFINEDFNISRTFKFQNFLSNKSIEKDVNEFTKLAANKIYQIPNE", "aa_hexdigest": "8b31c4e311e68b793125421664055c86", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10982.145999999988, "isoelectric_point": 5.678635978698731 }, "id": "DFOKDJOJIG_847", "locus": "GCA013367855_00837" }, { "type": "cds", "sequence": "contig_1", "start": 478800, "stop": 479582, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGACTTTTTAAAGAGGTTTTCAAATCATTGGCAAGAAATAAAGTTACCTTGATTTGTTTAACAATTTTAATTTTCTTAACAACATTTTTATTTACTTTGTTAAATGATGTTACTACTAGCTATTCATCTACGATTAATTCGTATGAAAAGGTTTCAAAATTGCATGATGCAACTGTTGATTTTGACGTTAACCCTTCAGGTGTGATACCAAAATCAGGTTATAACCAAATAGGATCTGATAATCAATCAATTGTTGATAAACCTGTTGTTTTTGAGTCTTCTAATAATGGAAGTGATATTTCATATTCAATAGATCTACCAAAAGATCAAAGAAATTATGTAGGTTTAAAAAATACTTTTAATAATTTAAATTATGATAATGAAAACCATTTTATAAATACTTATGATTTATTAAATTTATTATATTCTAGCAAGGAATCTATAAGTGATGTAGACGTTGAAATTTTAAAATCCAACCCTGATTTTTCTAAAATTAGGAATTTTAGTTTTTCGGGTCATGATAGGGAATTTAAACTTTATGTAAAAGATAGCAAAGGAAATATAAAACCATTAACTAAAAGATATAATTTGACAAAAAATGATGACATTACTTTTTTAAATGAAGTTAGATTAAAAAATATAGCTTCTATTGCATATGGTCCAAGAGGCTCGCTAAACGATCAAAAAGTAGTTGATTATTTATACAATCCCTCTCCATTATTTCTAAATGTTAAAACTAAACAAGCATCATTTGATACTAAAGATTATGAAGCATGA", "aa": "MRRLFKEVFKSLARNKVTLICLTILIFLTTFLFTLLNDVTTSYSSTINSYEKVSKLHDATVDFDVNPSGVIPKSGYNQIGSDNQSIVDKPVVFESSNNGSDISYSIDLPKDQRNYVGLKNTFNNLNYDNENHFINTYDLLNLLYSSKESISDVDVEILKSNPDFSKIRNFSFSGHDREFKLYVKDSKGNIKPLTKRYNLTKNDDITFLNEVRLKNIASIAYGPRGSLNDQKVVDYLYNPSPLFLNVKTKQASFDTKDYEA", "aa_hexdigest": "fb44791a41c3b590b0d76a01b9942aae", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29705.062000000053, "isoelectric_point": 7.699773597717285 }, "id": "DFOKDJOJIG_848", "locus": "GCA013367855_00838" }, { "type": "cds", "sequence": "contig_1", "start": 479767, "stop": 480447, "strand": "-", "frame": 1, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "COG:COG0357", "COG:J", "EC:2.1.1.170", "GO:0008168", "GO:0031167", "GO:0046118", "KEGG:K03501", "SO:0001217", "UniRef:UniRef50_D1J8E2", "UniRef:UniRef90_D1J8E2" ], "nt": "ATGCTTACGGAAAAAGAAAGCAATAATTTAATATTGCAAAGTCTACCACAATGTAGCAAAGAAGTCTTAAAAAAATTATATGAATATTATCAATTAATAGAACAAGAAAATAGCAAATATAATTTAACAGGTTTCTATAACGAAAAGCTTTTAAAAGAAGCAATAATTGAATCAATTTTAATTTTTTTAGACATTGAAAAAAATATTTGTAATCTAACTAACAAATCTGTACTCGACATTGGCTCGGGCGCAGGATTTCCTATCCTTCCATATTTCATATATAAACAAGATTTTTCTTTAACAATTTATGAGCCTCAATCAAAAAGAGTAAATTTTTTAAATTTGGTTAAAAATGAGTTGAAATTGAAAAATATTGATATACAAAAAATTCGTGCTGAAGATTCAAATCAAATAGAATGTTTTGATTTTATTAGTGCAAGAGCGGTGAGTGAATTAAAAAATTTATTAGAAATTTCACACCATCTTTTAAAAATTAATGGAATTGCTTGCTTTTTAAAATCAAATAAATATCAACAAGAAATAGCTAATTCAAACTTTATAGTTAACAAATTAAATATTAATTTAAAAACAAGGATTTTGCCAATATATTTCAATATTGAAAATGTTTTAATTTTTCACCAAAAATTAATAAAAACAGCCTTAGGATTTCCTATAAAATGA", "aa": "MLTEKESNNLILQSLPQCSKEVLKKLYEYYQLIEQENSKYNLTGFYNEKLLKEAIIESILIFLDIEKNICNLTNKSVLDIGSGAGFPILPYFIYKQDFSLTIYEPQSKRVNFLNLVKNELKLKNIDIQKIRAEDSNQIECFDFISARAVSELKNLLEISHHLLKINGIACFLKSNKYQQEIANSNFIVNKLNINLKTRILPIYFNIENVLIFHQKLIKTALGFPIK", "aa_hexdigest": "e957ac97f164b1e014df9e3293c3c4e7", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_D1J8E2", "query_cov": 1.0, "subject_cov": 0.9495798319327731, "identity": 0.982, "score": 429.0, "evalue": 1.46e-151, "valid": true, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.170" ], "uniref50_id": "UniRef50_D1J8E2", "cog_id": "COG0357", "cog_category": "J", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0008168", "GO:0031167", "GO:0046118" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8E2" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "DFOKDJOJIG_849", "locus": "GCA013367855_00839" }, { "type": "cds", "sequence": "contig_1", "start": 480422, "stop": 481396, "strand": "-", "frame": 3, "gene": "prsA", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "KEGG:K00948", "RefSeq:WP_012855629.1", "SO:0001217", "UniParc:UPI0001BC237C", "UniRef:UniRef100_D1J8E3", "UniRef:UniRef50_Q98R83", "UniRef:UniRef90_D1J8E3" ], "nt": "ATGAAAGCAAAAAAAGAAGATAATGTTGTAATTTTTGGCATGGAAAATTCCCAAAAATTAGCACAAGAAATTGGAAAGTATTTAAATAAAGAAATATTGCCAGTTAAAACTACAGTATTTGCAGATGGAGAATGCCTTTTAAAATCAGAAACAACCGTTAGAAATAAGGATGTTTACATTATTTGCAATACCTCAAAACCAGTAAACGATAACATAATGTCATTGCTGATTTTAATTGATTCATTAAGACGTTCATCAGCAAAGAGCATTAATTGCGTTATGACCTACTATGGATATGCAAGACAAGATAGAAAAGCAAGTGGAAGAGATCCAATATCAGCTGCACTAGTTGCTAAGATGTATGAAACAGCGGGTGCTGATAAAATAATTACTATTGACTTGCACAACGCTTCAATTCAAGGATTTTTCAACATTCCTGTTGATGATTTAAGGGGACAATTTATTTTTTCAAATGAATTGCTAAACAGAAAAGAAAAACTAGTAATTGTATCACCAGACCACGGGGGTGCAGTTAGAGCAAGACAATTGTCAGAATTACTAAACAATGAAGAACAAATTGCAATTATTGACAAGCGTCGTACAGGACCTAATAAATGCGAAATTATGGGTGTTTTAGGTGATGTTAAAGATAAAAATGTTTTAATCTATGACGATATTATTGACACAGGTGGAACAATTATTCATGCAGCAGAAGCTTTAAAAGCAAATGGCGCAAAAAATATTATAATTGCAGCCACTCATGGTTTATTTTCAAAAGGATTTGAAATGTTTGAAAATAACGCAATGATTGATGAAGTTATTATTACTAATTCAATAGATCACAGCCATTTATCAAACTTTAAAAAATTAAGAGAATTATCTTTGGCTCAATTTTTAAGTATGGTAATCAATGCAAACATTGAAAGCAAATCTATAACAGAAGTATATAATGCTTACGGAAAAAGAAAGCAATAA", "aa": "MKAKKEDNVVIFGMENSQKLAQEIGKYLNKEILPVKTTVFADGECLLKSETTVRNKDVYIICNTSKPVNDNIMSLLILIDSLRRSSAKSINCVMTYYGYARQDRKASGRDPISAALVAKMYETAGADKIITIDLHNASIQGFFNIPVDDLRGQFIFSNELLNRKEKLVIVSPDHGGAVRARQLSELLNNEEQIAIIDKRRTGPNKCEIMGVLGDVKDKNVLIYDDIIDTGGTIIHAAEALKANGAKNIIIAATHGLFSKGFEMFENNAMIDEVIITNSIDHSHLSNFKKLRELSLAQFLSMVINANIESKSITEVYNAYGKRKQ", "aa_hexdigest": "4ad11e3f20055d1b39cae4c28e53011b", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC237C", "ncbi_nrp_id": "WP_012855629.1", "uniref100_id": "UniRef100_D1J8E3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC237C", "RefSeq:WP_012855629.1", "UniRef:UniRef100_D1J8E3" ] }, "ips": { "uniref100_id": "UniRef100_D1J8E3", "uniref90_id": "UniRef90_D1J8E3" }, "psc": { "uniref90_id": "UniRef90_D1J8E3", "gene": "PrsA", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_Q98R83", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948" }, "pscc": { "uniref50_id": "UniRef50_Q98R83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R83" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [ "prsA" ], "id": "DFOKDJOJIG_850", "locus": "GCA013367855_00840" }, { "type": "cds", "sequence": "contig_1", "start": 481591, "stop": 481779, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACTCAAACCCTTGTAATTCACTTTTTGCGTACTGACAAAACACCATTTATTAAATCAAATGCAACAACTTCGTTATTTATTTCAACATTAATTGGATGTGCAATAATTAATGCCGTGCCATATATACCAGGAATTAATAAAGGTTTAAGTCTAATGCCATTACCAGGAATTTATTATGCTTGA", "aa": "MYTQTLVIHFLRTDKTPFIKSNATTSLFISTLIGCAIINAVPYIPGINKGLSLMPLPGIYYA", "aa_hexdigest": "5bb3a49d8d55cdb133e821f609318a28", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6804.0436, "isoelectric_point": 9.360567665100099 }, "id": "DFOKDJOJIG_851", "locus": "GCA013367855_00841" }, { "type": "cds", "sequence": "contig_1", "start": 482062, "stop": 483366, "strand": "-", "frame": 1, "gene": null, "product": "Magnesium-transporting ATPase, P-type 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X4XS16" ], "nt": "TTGTTTGGTATATCAATTGCTGTTGGTTTAACACCGGAAATGCTTCCAATGATAGTGACAGCAAGTTTATCTAAAGGTGCAGTTTCACTAGGCAAGGCCAAAACAATAATTAAAAATATTAATTCAATTCAAAATTTTGGTTCAATGGACATTTTATGTACAGATAAAACTGGAACATTAACACAAGATGAAGTAATTATGGAACGCCATATGGATGTTACCGGCAAAGAAGATTTTCATGTTTTAAAATATGCTTTTATTAATTCATATTATCAAACAGGATTAAAAAATTTACTTGATAAATCAATAATTAATAAGACTTTTGAAATGGCATCAAAAAATAAAGAACTTGAGGATTTTCAAAGATTTTATGAAAAAATTGACGAAATTCCTTTTGATTTCAAAAGAAAAAGAATGTCTGTTTTAGTTAAATCATTAAAACAAAATAAAGTAACCCTAATTACCAAGGGTGCTGTCGAAGAAATTGTGAGTGTATGTAAAAATATTTACATTGATGGAGAGGTAAAACCTCTTGATAAAAAAACAATAGATAAGGTTCTAAGAAGAGTTGAAGAATTCAATTCAGAAGGACTAAGAGTTATTGCAATAGCTTCTAAAAAATCAACAATTAATGCAGTTGGAAAAATATCGGTAAATGATGAATGCGATATGACGTTGATTGGATATTTAACATTTCTTGATCCTCCTAAAGATTCTGCTGCTGCTGCAATTGAAAAATTGCATGAATATGGAGTTGATGTAAAAATATTAACAGGAGATAGTGCACTTGTAACAAAAGCAGTATGTGCCCAAGTAGGAATAAAAAATAATAGAATTTTACTAGGCAAAGAAGTTGAAGAATTAAGTGATGCTGAACTTAAAATTGAAGTTGAAAAAGTTCAAATTTTTGCAAAATTAAGCCCTGACCAAAAGGCAAGAATTATTTCAATATTGCGTGATAATGGTCATGTTGTTGGCTATATGGGTGATGGAATTAATGATGCACCCGCTATGAAATCGGCCGATGTTTCAATATCAGTCGATACTGCGGTTGATATTGCAAAAGAATCAGCTAACATAATTCTTTTAGAAAAAGACCTAAACGTTTTGGCTAAAGGAATTGTTGAAGGTAGAAAAACACATGCCAATATTAACAAATATATAAAAATGACTGCATCTTCAAACTTCGGAAATATATTTAGTATTTTAATTGCTTCATTTTTAATTCCTTTTGTTCCATTAAACCCTGTACAAATAATATTATTGAATTTGGTATATGATGTTTGCTGTGCAACAATGCCATGA", "aa": "MFGISIAVGLTPEMLPMIVTASLSKGAVSLGKAKTIIKNINSIQNFGSMDILCTDKTGTLTQDEVIMERHMDVTGKEDFHVLKYAFINSYYQTGLKNLLDKSIINKTFEMASKNKELEDFQRFYEKIDEIPFDFKRKRMSVLVKSLKQNKVTLITKGAVEEIVSVCKNIYIDGEVKPLDKKTIDKVLRRVEEFNSEGLRVIAIASKKSTINAVGKISVNDECDMTLIGYLTFLDPPKDSAAAAIEKLHEYGVDVKILTGDSALVTKAVCAQVGIKNNRILLGKEVEELSDAELKIEVEKVQIFAKLSPDQKARIISILRDNGHVVGYMGDGINDAPAMKSADVSISVDTAVDIAKESANIILLEKDLNVLAKGIVEGRKTHANINKYIKMTASSNFGNIFSILIASFLIPFVPLNPVQIILLNLVYDVCCATMP", "aa_hexdigest": "5b437bb35f2803d66af8e657cae419d2", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0001C17514", "query_cov": 0.9976958525345622, "subject_cov": 0.9039665970772442, "identity": 0.527, "score": 445.0, "evalue": 1.15e-150, "valid": false, "gene": "mgtA", "product": "magnesium-translocating P-type ATPase", "ec_ids": [ "7.2.2.14" ], "uniref50_id": "UniRef50_A0A2X4XS16", "kegg_orthology_id": "K01531", "go_ids": [ "GO:0015444", "GO:0015693", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2X4XS16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X4XS16" ], "product": "Magnesium-transporting ATPase, P-type 1" }, "genes": [], "id": "DFOKDJOJIG_852", "locus": "GCA013367855_00842" }, { "type": "cds", "sequence": "contig_1", "start": 483379, "stop": 483939, "strand": "-", "frame": 1, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "nt": "ATGGTTTTTTTAAGTGGAATTATTCACTTCGTTCAAGAACAACGTGGGGCATCAAGCGCTGATAAATTAGTTGAGACTGTTGAAACCACTTCGCTTGTTCAAAGGAATGGTATTATGAAGGAAATTCCAATGGATGAGATTGTAGTAGGCGATATTGTGTATTTAGCAGCTGGAGATATTATTCCGGCGGATGTAAGAATATTAGAAGCAAAAGACTTATTTATTTCTCAATCCTCATTAACCGGAGAAAGCGATGCTATTGAAAAATATAAGAACATCGACAGTTCTAAAGAATTTGAAGCAATAACTGACCGTAGTAATTTAGCTTTTATGGGAACTAATGTTATTTCAGGTTCTGCTAAAACTATTGTAATTGCAACCGGCAATGATACAATTATTGGACAAGTTGCTTCTAAAATCAATGAAAAACCAATACCAACCAACTTTGATAAAGGAATTAAAAAAATTTCAATAATGTTCACTATTATAATGGCTGTTATTGTTCCTATAGTATTTTTAATTTTAGGAATTAAAGGTGTTATAGGTGGAGAAAATCAATGA", "aa": "MVFLSGIIHFVQEQRGASSADKLVETVETTSLVQRNGIMKEIPMDEIVVGDIVYLAAGDIIPADVRILEAKDLFISQSSLTGESDAIEKYKNIDSSKEFEAITDRSNLAFMGTNVISGSAKTIVIATGNDTIIGQVASKINEKPIPTNFDKGIKKISIMFTIIMAVIVPIVFLILGIKGVIGGENQ", "aa_hexdigest": "068c32b7a2457ef169dae19419f14c69", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AC587A1", "query_cov": 0.9247311827956989, "subject_cov": 0.900523560209424, "identity": 0.541, "score": 157.0, "evalue": 1.48e-45, "valid": false, "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI002AC587A1" }, "pscc": { "uniref50_id": "UniRef50_UPI002AC587A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "product": "HAD-IC family P-type ATPase" }, "genes": [], "id": "DFOKDJOJIG_853", "locus": "GCA013367855_00843" }, { "type": "cds", "sequence": "contig_1", "start": 483950, "stop": 484171, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGACAGTCATTCCATCAATTCATGTTATCTTTTTGCCTTTTGATGCAGGCAATATTAAATCAATAATCATAGAAACAATTGCCAATGCAAACAAAATGATGTTAAAAAAATTAAAAAATGATCTAAATAAACGCTTTCAAACATTATCAGCGCCTTTTTTTGTTATTTTATTTAAACCATATTCTTCTCTTGCAGCTTCAATTCTTTCTTCATCTTGA", "aa": "MMTVIPSIHVIFLPFDAGNIKSIIIETIANANKMMLKKLKNDLNKRFQTLSAPFFVILFKPYSSLAASILSSS", "aa_hexdigest": "5fd2be3efd989f8b301e8dd44131c75e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8130.694099999997, "isoelectric_point": 9.998095512390137 }, "id": "DFOKDJOJIG_854", "locus": "GCA013367855_00844" }, { "type": "cds", "sequence": "contig_1", "start": 484090, "stop": 484317, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "ATGATAGTTAATATAACAAAAAATAAACTTAAAAATTCTTCAAGAAAAGAAAAACAAATTAAGCAAATCAATACAAGGTTAGTTGAAGCTTCTAATTGTAGTCAAGAAGAATTATTTTTAAAATATCAAACCTCTCAAAACGGATTTCAAGATGAAGAAAGAATTGAAGCTGCAAGAGAAGAATATGGTTTAAATAAAATAACAAAAAAAGGCGCTGATAATGTTTGA", "aa": "MIVNITKNKLKNSSRKEKQIKQINTRLVEASNCSQEELFLKYQTSQNGFQDEERIEAAREEYGLNKITKKGADNV", "aa_hexdigest": "d642ef878636ebb810d5a26cc4121806", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 40, "aa_cov": 0.52, "hmm_cov": 0.975, "evalue": 8.425491402172654e-06, "score": 21.067995071411133, "start": 32, "stop": 70 } ], "seq_stats": { "molecular_weight": 8690.704999999993, "isoelectric_point": 9.130415534973146 }, "id": "DFOKDJOJIG_855", "locus": "GCA013367855_00845" }, { "type": "cds", "sequence": "contig_1", "start": 484508, "stop": 484783, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGGTTGATACTTATTTTATTACAAAAAGTTGACATTATTTTAGCAGACGAACCTACCAATAGTCTTGATATAGCAAACGCAAGAGATGTTTTAGAATTATTGTCTTCAATATCACAAGAATACAAAAAAACAATTTTAGTAAATATTCACGACATTTCATTAATAAAAACTTATTTTAAGAAATACATTTATGTCAAAGATGGACAGATTATTGAAATTGGTGAAACAAAAAATTTAAATGAATTAAAAATAAAAGATTTATATAATTTTTAA", "aa": "MWLILILLQKVDIILADEPTNSLDIANARDVLELLSSISQEYKKTILVNIHDISLIKTYFKKYIYVKDGQIIEIGETKNLNELKIKDLYNF", "aa_hexdigest": "477e37b7292077b0aedcc44a98fda922", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10612.329799999989, "isoelectric_point": 5.278318977355956 }, "id": "DFOKDJOJIG_856", "locus": "GCA013367855_00846" }, { "type": "cds", "sequence": "contig_1", "start": 485021, "stop": 485341, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGCTGAGAAACATTGAGCAGAACACGCTTAATGTTTTTATTTTTGCAATATTTTGTTTTGTATTAATACATTCCAAATATAATTTAAATATGAAAAAAATTGAATTCATCAATGTTGATGCAAGTTATTATAAAAAAAGTCCAATAGTTTTAAAGAACATAAATTTTTCTATTGAAGAGGGCGAATTTATAGCGATAATAGGAAAATCCGGCGCTGGAAAATCGACAATTTTCAATGCTTTATTGAAGCAATTGTTTGTAAAAAAAGGCAATGTAAAAATTAATGATATTGATATTTATAGAGCTTCAAAAAAACAGTGA", "aa": "MLRNIEQNTLNVFIFAIFCFVLIHSKYNLNMKKIEFINVDASYYKKSPIVLKNINFSIEEGEFIAIIGKSGAGKSTIFNALLKQLFVKKGNVKINDIDIYRASKKQ", "aa_hexdigest": "4f385a0fa5460bb447980af87b0e7348", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 52, "aa_cov": 0.49056603773584906, "hmm_cov": 1.0, "evalue": 8.9028483338412e-15, "score": 51.158355712890625, "start": 51, "stop": 102 } ], "seq_stats": { "molecular_weight": 12166.234899999983, "isoelectric_point": 9.715530586242675 }, "id": "DFOKDJOJIG_857", "locus": "GCA013367855_00847" }, { "type": "cds", "sequence": "contig_1", "start": 485366, "stop": 485692, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02866.23" ], "nt": "ATGGTTAAAAAAGAAGCATTTGAAATTATTGCTGGCAAAGGCATTACTAATTATGGTATTGGCGAAAACCTTGCTGAAATTACGCAAGCAATTTTGTATGATAGACAAAGAATTTATTCTCTTGGAGTTCAATTACCAAAGGAATACAAACATGCCGGAGTTTACTTTGGTTTACCAGTAATCTTGGGTAAGAATGGTTATACACACTTGCCAAAAATTAGACTTGACGAAGATGAACAACAACAATTTGATGAATATTCAAAAGAAATTAAAGAAACAGCAATTCAAGTATTAAAAAATTTGGATGTTGAAATTACTATTAAATAA", "aa": "MVKKEAFEIIAGKGITNYGIGENLAEITQAILYDRQRIYSLGVQLPKEYKHAGVYFGLPVILGKNGYTHLPKIRLDEDEQQQFDEYSKEIKETAIQVLKNLDVEITIK", "aa_hexdigest": "0ff069a0685e97f7c251b507af1b4ee9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02866.23", "name": "Ldh_1_C", "length": 97, "aa_cov": 0.8611111111111112, "hmm_cov": 1.0, "evalue": 4.34836638787246e-15, "score": 51.76319122314453, "start": 2, "stop": 94 } ], "seq_stats": { "molecular_weight": 12343.04149999999, "isoelectric_point": 5.687104988098144 }, "id": "DFOKDJOJIG_858", "locus": "GCA013367855_00848" }, { "type": "cds", "sequence": "contig_1", "start": 485741, "stop": 486334, "strand": "-", "frame": 3, "gene": "mdh", "product": "L-lactate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0039", "SO:0001217", "UniRef:UniRef50_A0A2Z4LNL4", "UniRef:UniRef90_D1J8E6" ], "nt": "ATGACTAAAATAGGATTAATTGGCGTTGGTGCTGTTGGTAGTTCATATTTATATGCTGCATTAAATAAAAATATAGAAGCAGATTATGTTTTAATTGATAGTTTTCCACAAGTCGCAATTGCGCAAGCGAAAGATTTAAATGATGCTGCTTGTTCAATGGCCAACAATGGTTCAACATTCAAAGCTGGAGATTATTCAGATTTGAAAGATGCTGATATTGTTATTATAACTGCATCAATTAGACCAAAAGAAGGCGTTTTAAAAGATAGAATGGAATTATTGACCGATAATGCAAAATTATTGAGAAAAATCGGCCAAAGCATAAAAGATTCAGGTTTTGATGGTGTTACAATCATTGCCTCAAATCCTGTTGACATTATGGCAACAATTTATCAACAAGTAACCAAGTTTTCTCCAAATAAAGTTATAAGTTCAGGAACTATTCTAGAAACTGCAAGAATGAAAAAATTCCTTTCAGAAAAAATTGGAATTAAAGCACACTCAATTACTGGATTTGTTATTGGTGAACATGGAGCAAGATGTTTAATTCCATTTTCAAAAATAAGAATTGGTTTGGGTTCATTAAAAGATTGA", "aa": "MTKIGLIGVGAVGSSYLYAALNKNIEADYVLIDSFPQVAIAQAKDLNDAACSMANNGSTFKAGDYSDLKDADIVIITASIRPKEGVLKDRMELLTDNAKLLRKIGQSIKDSGFDGVTIIASNPVDIMATIYQQVTKFSPNKVISSGTILETARMKKFLSEKIGIKAHSITGFVIGEHGARCLIPFSKIRIGLGSLKD", "aa_hexdigest": "7a5bdf2457aff4b0cd56b60bf7ee2e79", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "mdh" ], "truncated": "5-prime", "pseudogene": { "start": 485438, "stop": 486334, "observations": { "mutation": [ 485713, 485743 ], "stop": [ 485713, 485743 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E6", "query_cov": 1.0, "subject_cov": 0.6118012422360248, "identity": 1.0, "score": 380.0, "evalue": 1.37e-131, "gene_start": 1, "gene_end": 197, "reference_start": 1, "reference_end": 197, "reference_sequence": "MTKIGLIGVGAVGSSYLYAALNKNIEADYVLIDSFPQVAIAQAKDLNDAACSMANNGSTFKAGDYSDLKDADIVIITASIRPKEGVLKDRMELLTDNAKLLRKIGQSIKDSGFDGVTIIASNPVDIMATIYQQVTKFSPNKVISSGTILETARMKKFLSEKIGIKAHSITGFVIGEHGARCLIPFSKIRIGLGSLKDWLANGTITNEWLSQLNEMVKKEAFEIIAGKGITNYGIGENLAEITQAILYDRQRIYSLGVQLPKEYKHAGVYFGLPVILGKNGYTHLPKIRLDEDEQQQFDEYSKEIKETAIQVLKNLDVEITIK" }, "paralog": false, "identity": 0.9932885906040269, "score": 574.0, "evalue": 2.93e-210, "description": "Internal stop codon at 485713, 485743. Nonsense mutation around 485713, 485743.", "psc": { "uniref90_id": "UniRef90_D1J8E6", "gene": "Mdh", "product": "L-lactate dehydrogenase", "uniref50_id": "UniRef50_A0A2Z4LNL4", "cog_id": "COG0039", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E6", "UniRef:UniRef50_A0A2Z4LNL4", "COG:COG0039", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z4LNL4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z4LNL4" ], "product": "L-lactate dehydrogenase" } }, "id": "DFOKDJOJIG_859", "locus": "GCA013367855_00849" }, { "type": "cds", "sequence": "contig_1", "start": 486471, "stop": 486806, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTCAGTGAGACCATTTCATCTATGAACAATAGGATTTATATTGAAACTTGCTTAGGGGGCGTTGATAACAAAACTTTGGCCTCAAATTACACCAACACTCCATTTAAGTGCTTTGATACTTGTTTAAAATTTCAAGAACCTATTATGGACTGCAAAATTTCACAAAATAACGAAAATGAGACGGATGGTTTGAATTATTTAAGCAATTTAACTTATAATAATTTATGTAATCTTGTAATGGAAACATTTATTGAAAACCATGTTAATGTATATAAAATACCTAACATTCAAATCATGATTGACAATTATAACGATAGTACATTGGGTTGA", "aa": "MLFSETISSMNNRIYIETCLGGVDNKTLASNYTNTPFKCFDTCLKFQEPIMDCKISQNNENETDGLNYLSNLTYNNLCNLVMETFIENHVNVYKIPNIQIMIDNYNDSTLG", "aa_hexdigest": "44a004cbcfe51d4238e8609598e405ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12742.246099999986, "isoelectric_point": 4.352127647399903 }, "id": "DFOKDJOJIG_860", "locus": "GCA013367855_00850" }, { "type": "cds", "sequence": "contig_1", "start": 486810, "stop": 487646, "strand": "-", "frame": 2, "gene": "pgi", "product": "Glucose-6-phosphate isomerase (GPI)", "db_xrefs": [ "COG:COG0166", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J8E7", "UniRef:UniRef90_D1J8E7" ], "nt": "ATGGAAAATAAGCAAATTATTGACATAAATTTAAAAAAAGCAATTAAAGAAAGTGCACCAAACCGTTATTATGAGGCGGTTCAAAAAATCTTGTTAGATATAAAAAACTCAAAGGTTAAAAATTCAAATTATTTAGGTGTATTCAATGTTTTTAAAAATGTAAAGAAATCTGATTATGAAAAAATTCAAGAAATATCAAGTGAATTATTATCAGATGGTATTGAATATTTAGTAATAATAGCAACTCAAACAATAATTAATCAAATTAAGGCGCTTGCTGATTTACATTTTGATTTAAGAAAAGAAAATGAATCAATTAAATTGGTTTATGTTAATGAAAATTTTAATAGTTTAGAAGTTGCTAAATTGCTTATTAAACTTCAAAATAAAAAGTTTGCTATTAATGTAATCTCACAAACTGGCGAATGCTTAGAAACATTAATTATGTTTAGAGAATTAAAAAACTTATTATATAAAGAGTTAGGCAAGGCGGGCGCTTCTAAATATATCTATGTAACTACAAACAATAATTATGGAAGATTATTTAATGATGCCAAGATAAATCAATATACTCATTTAACAATTTTAGATAATACAGTCGAAAGATTCTTAAGCTATTCACCTGCAATTTTATTGCCAATTGCTTGTATAGGAATTAATATTGAAAAATATTTATCTGGTGCTCATAAGGCTAATGAATTTTTTTTAAATGCACCATTAGAAAAAAACTCAGCAGTTTTATATGCAATTACTAGAAAAATTCTTCATGATAATAATTTTATATTTGAAAATGTTTGCTATTTCGAGCAAAATCAAGAATCCTTAGCAAAATTGTGA", "aa": "MENKQIIDINLKKAIKESAPNRYYEAVQKILLDIKNSKVKNSNYLGVFNVFKNVKKSDYEKIQEISSELLSDGIEYLVIIATQTIINQIKALADLHFDLRKENESIKLVYVNENFNSLEVAKLLIKLQNKKFAINVISQTGECLETLIMFRELKNLLYKELGKAGASKYIYVTTNNNYGRLFNDAKINQYTHLTILDNTVERFLSYSPAILLPIACIGINIEKYLSGAHKANEFFLNAPLEKNSAVLYAITRKILHDNNFIFENVCYFEQNQESLAKL", "aa_hexdigest": "53ebebec50e3429bcb0de28b82b42244", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "pgi" ], "truncated": "5-prime", "pseudogene": { "start": 486507, "stop": 487646, "observations": { "mutation": [ 486812 ], "stop": [ 486812 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E7", "query_cov": 1.0, "subject_cov": 0.6303854875283447, "identity": 0.9890000000000001, "score": 530.0, "evalue": 1.52e-187, "gene_start": 1, "gene_end": 278, "reference_start": 1, "reference_end": 278, "reference_sequence": "MENKQIIDINLKKAIKESAPSRYYETVQKILLDIKNSKVKNSNYLGVFNVFKNVKKSDYEKIQEISSELLSNGIEYLVIIATQTIINQIKALADLHFDLRKENESIKLVYVNENFNSLEVAKLLIKLQNKKFAINVISQTGECLETLIMFRELKNLLYKELGKAGASKYIYVTTNNNYGRLFNDAKINQYTHLTILDNTVERFLSYSPAILLPIACIGINIEKYLSGAHKANEFFLNAPLEKNSAVLYAITRKILHDNNFIFENVCYFEQNQESLAKLWQMLFSETISSMNNRIYIETCLGGVDNKTLASNYTNTPFKCFDTCLKFQEPIMDCKISQNNENETDGLNYLSNLTYNNLCNLVMETFIENHVNVYKIPNIQIMIDNYNDSTLGWIMSFLHHSSILFAYLNEQNPFERKPLESYNFNLAKNFKELLGGKKKWLK" }, "paralog": false, "identity": 0.9894459102902374, "score": 744.0, "evalue": 4.13e-274, "description": "Internal stop codon at 486812. Nonsense mutation around 486812.", "psc": { "uniref90_id": "UniRef90_D1J8E7", "gene": "Pgi", "product": "Glucose-6-phosphate isomerase (GPI)", "uniref50_id": "UniRef50_D1J8E7", "cog_id": "COG0166", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E7", "UniRef:UniRef50_D1J8E7", "COG:COG0166", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8E7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8E7" ], "product": "Glucose-6-phosphate isomerase (GPI)" } }, "id": "DFOKDJOJIG_861", "locus": "GCA013367855_00851" }, { "type": "cds", "sequence": "contig_1", "start": 487636, "stop": 487875, "strand": "-", "frame": 1, "gene": "yabR", "product": "30S ribosomal protein S1", "db_xrefs": [ "COG:COG1098", "COG:R", "SO:0001217", "UniRef:UniRef50_A0A3T0TTP6", "UniRef:UniRef90_D1J8E8" ], "nt": "TTGAGTATAAATGAAGTTAGTGATTATTACATTAGCGATTTAAAATTAATGTTTAAAATCAATGATATTAAGGAAGTTCAAGTAATTGAAATAATGCCAAATAATGATTTAGTAGTTTCATTTAAAAGAATTCACCCCAAGGAATTAAGAAACCCTTTTGAATTTAAACTAGATAGTCAAGATGCAGAATTTGATGGATTATTAGAATTTACTAACAAAGGAATTAATTATGGAAAATAA", "aa": "MSINEVSDYYISDLKLMFKINDIKEVQVIEIMPNNDLVVSFKRIHPKELRNPFEFKLDSQDAEFDGLLEFTNKGINYGK", "aa_hexdigest": "e2ea816ef770b351b97bd2726dfdb568", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "yabR" ], "truncated": "3-prime", "pseudogene": { "start": 487636, "stop": 487971, "observations": { "mutation": [ 487923 ], "stop": [ 487923 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E8", "query_cov": 1.0, "subject_cov": 0.7117117117117117, "identity": 0.987, "score": 159.0, "evalue": 2.4e-49, "gene_start": 1, "gene_end": 79, "reference_start": 33, "reference_end": 111, "reference_sequence": "MNYEIGDIFKAKIIKVWKNNIILQTKEGCKCFLSINEVSDYYISDLKLMFKINDIKEVQVIEIMPNNDLVVSFKRIHPKELRNPFEFKLDSQDAEFDGLLEFTNKGINYGK" }, "paralog": false, "identity": 0.990990990990991, "score": 222.0, "evalue": 1.47e-76, "description": "Internal stop codon at 487923. Nonsense mutation around 487923.", "psc": { "uniref90_id": "UniRef90_D1J8E8", "gene": "YabR", "product": "30S ribosomal protein S1", "uniref50_id": "UniRef50_A0A3T0TTP6", "cog_id": "COG1098", "cog_category": "R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E8", "UniRef:UniRef50_A0A3T0TTP6", "COG:COG1098", "COG:R" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3T0TTP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3T0TTP6" ], "product": "S1 motif domain-containing protein" } }, "id": "DFOKDJOJIG_862", "locus": "GCA013367855_00852" }, { "type": "cds", "sequence": "contig_1", "start": 488458, "stop": 488655, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTTATAACGTCAACTTTATTAAAAAATTCATTGCTAAAAAATGAAGTTATTCCTTTACTTCAAACAAAAACAAGGGCTTTTTATTTTGATAAATTAGATGAAAAATATGTTGATGGTTCTGGCAATGATCCGCTAAGAAAAATAGCAAAAATAGCAAACGATTTATTTAACAAAAAGCCTTATAAATCATGA", "aa": "MAFITSTLLKNSLLKNEVIPLLQTKTRAFYFDKLDEKYVDGSGNDPLRKIAKIANDLFNKKPYKS", "aa_hexdigest": "1368cd42840ef97afa4b8bf2ec7153aa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7465.6231000000025, "isoelectric_point": 9.646807289123537 }, "id": "DFOKDJOJIG_863", "locus": "GCA013367855_00853" }, { "type": "ncRNA-region", "class": [ "riboswitch", "SO:0000035" ], "sequence": "contig_1", "start": 489343, "stop": 489430, "strand": "+", "label": "TPP", "product": "TPP riboswitch aptamer (THI element)", "score": 57.5, "evalue": 4.2e-07, "db_xrefs": [ "RFAM:RF00059", "SO:0000035" ], "nt": "TATAAAGAACAGGGGTGCCTTTGGCTGAGAAATACCCTCATTAGCTGATCTAGTTAATACTAGCGTAGTGAGTGTCTTAGTTTTTTGC", "id": "DFOKDJOJIG_42" }, { "type": "cds", "sequence": "contig_1", "start": 489455, "stop": 489604, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAATTTAAAAAATATTTGCTTATAGGGGCAGCGGTATTGCCTGCAATGGTTGGCGCATCAGTTTTATCTTCATCATGCGTTGATAAAATTTCAAACAATACTAAAACTTACGAAAATTTAAAATTTTCAATTAACAAGCCTTGA", "aa": "MLKFKKYLLIGAAVLPAMVGASVLSSSCVDKISNNTKTYENLKFSINKP", "aa_hexdigest": "600996eede73b2d80f6ca4e7b8329204", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5334.320699999999, "isoelectric_point": 9.732872581481931 }, "id": "DFOKDJOJIG_864", "locus": "GCA013367855_00854" }, { "type": "cds", "sequence": "contig_1", "start": 489776, "stop": 489955, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTACAATCTAGAAAAAGATAAATCAAATATTCATCCATTTTTGCAAACAATGACAAGAGCGTTTGTTTTTGATCAAGATTTTAACGCTCAATATTCAAATGGGTTAGATGACGATCCACTAAGAAAAATAGCTCAATCAGCATTTGATTTATTTAACAAAAAACCTTTTGAAAATTGA", "aa": "MYNLEKDKSNIHPFLQTMTRAFVFDQDFNAQYSNGLDDDPLRKIAQSAFDLFNKKPFEN", "aa_hexdigest": "29056b1ccd79ce8e4a91692201dfe7df", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6975.697100000001, "isoelectric_point": 5.030216789245605 }, "id": "DFOKDJOJIG_865", "locus": "GCA013367855_00855" }, { "type": "cds", "sequence": "contig_1", "start": 490133, "stop": 490507, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGGAAGTAGTTCTAGTGGTTCAAAATACTTGCTTCAAGAAGCATTATTTAAAAAACATTTTAATTTAAAAGGAAATGAATTTAAATCATTTTTAGAAGATAAACAAGGAAACGAAACAAAATACACCAAAGGTAAGATTAAAAATATTGGAAAAGGCGCATTTTCTAAATACCATATTGTTTTTGATGACCTTGGATCTTTTGCATATACTCATAGTAAAGGCAGCACAATCTATAAATTGGATGACAAAAATTCAAAAATTGAATTTTTAACAGTTACCGAACCTTTAAAATATAACATTCTTGCTACATCATCAAATATTCCAAAAGAATTAAGGGAAATAATTGCAAATGCAATGGTCGAAGTTTGA", "aa": "MHGSSSSGSKYLLQEALFKKHFNLKGNEFKSFLEDKQGNETKYTKGKIKNIGKGAFSKYHIVFDDLGSFAYTHSKGSTIYKLDDKNSKIEFLTVTEPLKYNILATSSNIPKELREIIANAMVEV", "aa_hexdigest": "f8c757b9527bdfb13f1caa200f14cf79", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13988.791099999982, "isoelectric_point": 9.30170803070068 }, "id": "DFOKDJOJIG_866", "locus": "GCA013367855_00856" }, { "type": "cds", "sequence": "contig_1", "start": 491344, "stop": 491655, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCTATCCAAATTCAATCAATGTCAATTACTTTCATGAACTTCTAATTCCACTACTAATATTGGCAACATTTTTAATTTTTATTGAATTTTGTAACTTTATAATATCAAATTTGTTGTTTTATTCAAGGACTCAAAAAGTAAATATAAAAAAATATGCCAAAAATTCTACTATTAAAAAAATAATAATTTTTATAACAATTGCAATAGGATTAATAATAACTATCTTTGCATGTTTATCATTAAGGGGAGAAAAATTTTATTCAACCCAAGGAAATTACTATTTCAAGGAATTATTTAATGGTGAATGA", "aa": "MIYPNSINVNYFHELLIPLLILATFLIFIEFCNFIISNLLFYSRTQKVNIKKYAKNSTIKKIIIFITIAIGLIITIFACLSLRGEKFYSTQGNYYFKELFNGE", "aa_hexdigest": "d33988eb3e7255c8bf672bd72f04265e", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12062.244199999985, "isoelectric_point": 9.329364967346194 }, "id": "DFOKDJOJIG_867", "locus": "GCA013367855_00857" }, { "type": "cds", "sequence": "contig_1", "start": 491887, "stop": 492213, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAATAAATTAGAAGCTGCTTTTGTTATAGCATTTGCAATTCATAGTTCTTCTACTATTTCTAGAATGTTGGAGAGCTCCATTAAAAAAATCTCGGCAAACAAGATAAATGAATTAAAAATCAAAGGATATTCAAAATTCTATATCTTTAGAAATTTCATAATGCCAACAATTAAGCTAGATTTAATTACATTTATGACTTTTGAAATTGAAAAAATAACTAGAAACTTTATTACATATGGTTTATTTTCTGCTTCATTATTAGGATCTGAAACTAATCTAGGAAGATATAAAGAAATCTCTGATATTGCACCATATTTATGA", "aa": "MFNKLEAAFVIAFAIHSSSTISRMLESSIKKISANKINELKIKGYSKFYIFRNFIMPTIKLDLITFMTFEIEKITRNFITYGLFSASLLGSETNLGRYKEISDIAPYL", "aa_hexdigest": "88debee0f7b0cf8942e5678eb3c67688", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12406.461199999983, "isoelectric_point": 9.55900135040283 }, "id": "DFOKDJOJIG_868", "locus": "GCA013367855_00858" }, { "type": "tRNA", "sequence": "contig_1", "start": 492459, "stop": 492532, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 64.3, "nt": "AGGGGTTTAGTATAACGGCAGTACCGCAGTCTCCAAAACTGCTTGTGAGGGTTCGATTCCTTTAACCCCTGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 492491, 492493 ], "id": "DFOKDJOJIG_22", "locus": "GCA013367855_00859" }, { "type": "cds", "sequence": "contig_1", "start": 492676, "stop": 493296, "strand": "-", "frame": 1, "gene": null, "product": "Methionine adenosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377WSC8" ], "nt": "ATGAAAAGCCAAGTAACATTGGAATATGATGATGAAAGCAATAAAATAACTGTTAAAAAAGTTTTAATGTCAATTCAACATGCTGAAAATGCAAATTTAAAAGAATTTAAAGATTTTATAAAGAAAAATATTGTTGATTATGTGTTAAAAGAATTTAATTTCAATTTGGATTATGATTTATTGATTAATCCGACTGGCAGATTTGTAATTGGAGGCCCTGTTGGAGATACTGGACTTACAGGAAGAAAAATAATTGTTGATACATATGGCGGTTTTGCTCACCATGGTGGAGGAGCATTTTCAGGGAAAGATGCTACAAAAATAGATAGGTCTGCTGCTTATATGGCAAGGTATATTGCTAAAAACTTAGTTGCTGCTAAGATAGCAGACAAGATTGAAATTCAATTGTCTTATGCAATAGGCTTGCCACGTCCTCAATCTATTTATATAAATTCATTTAATTCATCACAATATAATGATGAACAAATTGCTGACATTATTGAACATGTATTTGATTTAAGCGTTGAACAAATTATAAAAAAATTAGATTTATTAAAGCCGATATATCTACAAACTGCAACATATGGACATTTTGGTAGAAACGACCTTAATTTAAGTTGA", "aa": "MKSQVTLEYDDESNKITVKKVLMSIQHAENANLKEFKDFIKKNIVDYVLKEFNFNLDYDLLINPTGRFVIGGPVGDTGLTGRKIIVDTYGGFAHHGGGAFSGKDATKIDRSAAYMARYIAKNLVAAKIADKIEIQLSYAIGLPRPQSIYINSFNSSQYNDEQIADIIEHVFDLSVEQIIKKLDLLKPIYLQTATYGHFGRNDLNLS", "aa_hexdigest": "c710413c409e911dbd567aebf832970c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0010E99B0D", "query_cov": 0.9902912621359223, "subject_cov": 0.9577464788732394, "identity": 0.5539999999999999, "score": 233.0, "evalue": 8.48e-75, "valid": false, "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_A0A377WSC8", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0004478", "GO:0005524", "GO:0006556" ] }, "pscc": { "uniref50_id": "UniRef50_A0A377WSC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377WSC8" ], "product": "Methionine adenosyltransferase" }, "genes": [], "id": "DFOKDJOJIG_869", "locus": "GCA013367855_00860" }, { "type": "cds", "sequence": "contig_1", "start": 493558, "stop": 493764, "strand": "-", "frame": 1, "gene": null, "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387A9BEE" ], "nt": "ATGAAAAAATTATTAACATCAGAAAGTGTAGGGGCAGGTCACCCTGATAAAATATGTGACCAAATTAGTGATGCGATATTAGATAGTTTGTTGACAAATGATCCATATGCAAGAGTAGCTTGCGATGTTTTAGCAAACAACAACATCATTTATATAGGCGGTCAAATAACTACAAAGGCATATTCCGACACAATTAAAGAAGCATGA", "aa": "MKKLLTSESVGAGHPDKICDQISDAILDSLLTNDPYARVACDVLANNNIIYIGGQITTKAYSDTIKEA", "aa_hexdigest": "8e03e4d2757fc48613e17f520dcae434", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00387A9BEE", "query_cov": 1.0, "subject_cov": 0.9444444444444444, "identity": 0.5589999999999999, "score": 81.3, "evalue": 6.23e-19, "valid": false, "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_UPI00387A9BEE", "kegg_orthology_id": "K00789" }, "pscc": { "uniref50_id": "UniRef50_UPI00387A9BEE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387A9BEE" ], "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_870", "locus": "GCA013367855_00861" }, { "type": "cds", "sequence": "contig_1", "start": 494009, "stop": 494272, "strand": "-", "frame": 3, "gene": null, "product": "Aspartate--ammonia ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ9" ], "nt": "ATGTTGAATGGGGATTTAATTGTCTATGATTCTATAAATAAAAAAGCATTGGAAATTAGCAGTATGGGAATTAGAGTTGATAAAGATTCTTTAATTAAACAATCAAATAAAACCGAAGAAGAAATTAAAAAATTAAGTCCATATCATTGTGATTTAATAGAAGGCAAATTGCCATATACGATTGGTGGTGGCCTAGGCCAAAGTAGAATTGCAATGTTTCTATTAGAAAAACATCATATTGGTGAAGTTCAAGCATCTGCATGA", "aa": "MLNGDLIVYDSINKKALEISSMGIRVDKDSLIKQSNKTEEEIKKLSPYHCDLIEGKLPYTIGGGLGQSRIAMFLLEKHHIGEVQASA", "aa_hexdigest": "789ad7a5262b3c09fd370ed9a20c1ff5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZXQ9", "query_cov": 0.9655172413793104, "subject_cov": 0.8, "identity": 0.655, "score": 117.0, "evalue": 1.14e-32, "valid": false, "product": "Aspartate--ammonia ligase", "uniref50_id": "UniRef50_A0A448ZXQ9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ9" ], "product": "Aspartate--ammonia ligase" }, "genes": [], "id": "DFOKDJOJIG_871", "locus": "GCA013367855_00862" }, { "type": "cds", "sequence": "contig_1", "start": 494273, "stop": 494578, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "TTGATCATAAAAAAAGAAGATAGAAATTTAAAATATTTAAAACATATTGTTAAGTTAATTTATGGTGCATTAAAGAATACTGAAAAATCAATTAATAGAATCTATCCCGTTTTGAGTCAAAAGTTAGCTAAAAATATTTATTTTATTAGTTCTAGCCATTTATATAATCTTTATCCAAACCTAAGTCCTGAAGAACGTGAAGAAGCAATTTGTAAAGAATATGGTTCTGTTTTTATTTATCAAATAGGATACAATCTACCTGATGGAATTCCACATTCAAAAAGAGCATTCGACTATGATGATTGA", "aa": "MIIKKEDRNLKYLKHIVKLIYGALKNTEKSINRIYPVLSQKLAKNIYFISSSHLYNLYPNLSPEEREEAICKEYGSVFIYQIGYNLPDGIPHSKRAFDYDD", "aa_hexdigest": "df0729f8af031861047be0985d96d801", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 100, "aa_cov": 0.9900990099009901, "hmm_cov": 1.0, "evalue": 3.8478518351537504e-36, "score": 120.57884979248047, "start": 2, "stop": 101 } ], "seq_stats": { "molecular_weight": 11888.55049999999, "isoelectric_point": 8.90638790130615 }, "id": "DFOKDJOJIG_872", "locus": "GCA013367855_00863" }, { "type": "cds", "sequence": "contig_1", "start": 494708, "stop": 494938, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "ATGCCATCATCAAAATTATCATTGAAAGATACCCAATTTGCTATTGAAAAATTAAAAGATCTGTTTTCACATCAACTTCATAGAACCTTGAATTTGGTCAGGGTTTCAGCGCCATTGTTTGTAAAAACTAAGAGTGGTTTAAACGACGGTTTGAATGGAGAAAAACCAGTTGTCTTTTTTGCAGACGAAGTTGAAGATGAATTGCAAATTATTCATTCACTTGCCAAATGA", "aa": "MPSSKLSLKDTQFAIEKLKDLFSHQLHRTLNLVRVSAPLFVKTKSGLNDGLNGEKPVVFFADEVEDELQIIHSLAK", "aa_hexdigest": "4ebbf96f33764ac1b55f29343995a790", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 67, "aa_cov": 0.8552631578947368, "hmm_cov": 1.0, "evalue": 9.23847329061493e-20, "score": 66.99182891845703, "start": 12, "stop": 76 } ], "seq_stats": { "molecular_weight": 8533.764299999999, "isoelectric_point": 6.824282646179199 }, "id": "DFOKDJOJIG_873", "locus": "GCA013367855_00864" }, { "type": "cds", "sequence": "contig_1", "start": 495072, "stop": 495527, "strand": "-", "frame": 2, "gene": null, "product": "DUF885 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "nt": "ATGGATGCTGTAAAATTGGCTAACTTGTTGCAATACCAAGGTGCATTAAACGAAGCTCAATTGCGTAACATGCGTTTAGCATTAGACACTGCTTACCATGGTAAAGGTGTAAAAGGACATGAAGACTTGCCAGCAGGTGCTTCAATCAACCAAGTAAGAGAATTCATGAAGAAGAATTCATCACTAGGAATTGGTGACATAACATCAGAATCAAGACGTTACCTATCATATGTTGGTCAAGCAACCTCATATAACTCAGGTAAGGCAGTTATGATGGATCTATATACAAAAGTTCAAAAGAAACTAGGATTAACAAGAAGAGAATTTGTCGAAAAAGATAATCATAAATATGTAAAAGAATTCTTTGATGCTTTATTACGTAATAGTGCATTGCCAATGGATGCTTTAATTAAATCAGTAAGTGCTAAATATGGCCTAACTGTTGAAAAGAAATAG", "aa": "MDAVKLANLLQYQGALNEAQLRNMRLALDTAYHGKGVKGHEDLPAGASINQVREFMKKNSSLGIGDITSESRRYLSYVGQATSYNSGKAVMMDLYTKVQKKLGLTRREFVEKDNHKYVKEFFDALLRNSALPMDALIKSVSAKYGLTVEKK", "aa_hexdigest": "5e7d6e2594f9d508132513afbad2818f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P8H0", "query_cov": 0.8675496688741722, "subject_cov": 1.0234375, "identity": 0.55, "score": 139.0, "evalue": 5.74e-40, "valid": false, "product": "Bacterial protein of uncharacterized function (DUF885)", "uniref50_id": "UniRef50_A0A3B0P8H0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "product": "Bacterial protein of uncharacterized function (DUF885)" }, "genes": [], "id": "DFOKDJOJIG_874", "locus": "GCA013367855_00865" }, { "type": "cds", "sequence": "contig_1", "start": 495579, "stop": 495698, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGTTGACTTCTCAACAGCGCATGGTATAACAAACTTCTTTACTGCTAAAACAGAAGCAGAAGTTACAGATGACATGATTCAACAAATTAAAGACCTACACAATGGTGTATATTGA", "aa": "MPVDFSTAHGITNFFTAKTEAEVTDDMIQQIKDLHNGVY", "aa_hexdigest": "0fee1761c63433642153f643aeb86683", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4385.838, "isoelectric_point": 4.507241249084472 }, "id": "DFOKDJOJIG_875", "locus": "GCA013367855_00866" }, { "type": "cds", "sequence": "contig_1", "start": 495783, "stop": 495899, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGACACCACAACCAATTAATGTATGCTCAAAAGTATCTATCATCAGTTGGAGAATTTGGTAAATATAAATTAGGAAATGTATTCCACTACACTTCATACGTTGAAGGTTGA", "aa": "MMGHHNQLMYAQKYLSSVGEFGKYKLGNVFHYTSYVEG", "aa_hexdigest": "064ced3e7916d6f765f037258ca96559", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4415.979300000001, "isoelectric_point": 8.093570518493653 }, "id": "DFOKDJOJIG_876", "locus": "GCA013367855_00867" }, { "type": "cds", "sequence": "contig_1", "start": 495930, "stop": 496130, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGCATTTAAGGCATATGAACAATTTAAGAAGACTACTCAAAATTATGGACGTAAATTCTTTGCAAATGAAGTTCCTGATTATGATATTCAAACCTATAAATTTAATGAAAGAGAATTTGTGGGTGTTGGAGCTTACAACTCAGGCATTAAAAAATTCATGTTTAATGTTGACCCATACTTCTCATTACCAAAATGA", "aa": "MSAFKAYEQFKKTTQNYGRKFFANEVPDYDIQTYKFNEREFVGVGAYNSGIKKFMFNVDPYFSLPK", "aa_hexdigest": "147adea60c7d9936a5b44fb6d9f8664d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7842.782099999999, "isoelectric_point": 9.222540855407715 }, "id": "DFOKDJOJIG_877", "locus": "GCA013367855_00868" }, { "type": "cds", "sequence": "contig_1", "start": 496357, "stop": 496533, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCTTTTTCATCACGAATTACAACAGTTTCTTCTTTTATTTCTTCTGGTCCAATACCATCAGCATCGTATTTAATTTTAGCTTCTCATTTTTTGCCTTTTCCAACAATTAAATCAGCTGCTTGGTTAGCAATTTTCTTTGAGTTTTCAGCAGATTTCTTTGTTGTTTCATAA", "aa": "MFPFSSRITTVSSFISSGPIPSASYLILASHFLPFPTIKSAAWLAIFFEFSADFFVVS", "aa_hexdigest": "ea56eb80e1eb0db2d5e379dfa934a12c", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6382.334099999998, "isoelectric_point": 6.505302238464354 }, "id": "DFOKDJOJIG_878", "locus": "GCA013367855_00869" }, { "type": "cds", "sequence": "contig_1", "start": 496446, "stop": 496796, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGAAGATATTAAAGAATTAAAACTTTATAAAGAAAATAAAACAAACGAAAAAGAATTATCAATTGATGCTAGAGATAAAGGCGGAAAAATTGTTAAATTATATGGTTTAGGATTAACTGAAAAAGACTTAAATCAAAGAAATGTTGGTTTGGGATTTGCTGAAGGAGATGCTACCGTAAATGGTCAATCGATGTATCGTCAAATTCTAAAGATGGCTACAACTTCTGATTTAACAGATGATCAAGTAAACAATATTGGTTATGAAACAACAAAGAAATCTGCTGAAAACTCAAAGAAAATTGCTAACCAAGCAGCTGATTTAATTGTTGGAAAAGGCAAAAAATGA", "aa": "MGEDIKELKLYKENKTNEKELSIDARDKGGKIVKLYGLGLTEKDLNQRNVGLGFAEGDATVNGQSMYRQILKMATTSDLTDDQVNNIGYETTKKSAENSKKIANQAADLIVGKGKK", "aa_hexdigest": "275990912ffa5c6a1b2158b989bb66cf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12754.286399999984, "isoelectric_point": 8.707309532165528 }, "id": "DFOKDJOJIG_879", "locus": "GCA013367855_00870" }, { "type": "cds", "sequence": "contig_1", "start": 496851, "stop": 497138, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATCCTACTTTCTTTTATGGAAATGCTTCTGTATATCCAATGAATGCATTCAGCAATCAATACGGAAAGTATGGTCAATTACCATATAAACAATTATTAGCAAACTTTAAAGAAGCAGTTCAACATAAAATAGTTATGTCAAAAGTTTATTCAAAAATGGTAGTTAATGCTTTTGTTGGCAGACTATTCCAAGAAGAATTAACTAAGTTTGTTGAAGATAAATCTAAAAACGAAATATCTGTTGCAGATTTAATTGAATCATCATCATTGGAAGGAAATTGA", "aa": "MLNPTFFYGNASVYPMNAFSNQYGKYGQLPYKQLLANFKEAVQHKIVMSKVYSKMVVNAFVGRLFQEELTKFVEDKSKNEISVADLIESSSLEGN", "aa_hexdigest": "068913fa55ed9c7063b625b829be8797", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10812.255999999996, "isoelectric_point": 7.915379905700684 }, "id": "DFOKDJOJIG_880", "locus": "GCA013367855_00871" }, { "type": "cds", "sequence": "contig_1", "start": 497199, "stop": 498311, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAAACTAGAATTCTAATTTCATTAGGTTCAATTTTTGGCGTAGCAGCCTTAATGCCTATGGCAGCATCATGTACACATACAAATAATGATGAATTAAACAAAAAAATTGAATCTTTAGAAAAGCAATTAAATGAAGCAAAAGCAAATTCTCAAAAAGATCAAGCACAAATTAATGATCTTATGAAGCAATTGCAAGAAACAAAAAATAATTCAAAAACAATTGAAGAAAAAGAAAAATTAGTTAAAAAATTGCAAGAAGATTTAAATAAATTTCAACAAGAAAGAACAGCTAAATATCAAACAGAATTAAATGCTGCAAACAAGGCTATTGTTGAAATTCCTAACAAATATAAGGAACTAGAACAAAAAAATGATGATCTTAAAGATCAATTAGAAAACTTATCAAAAGAATTGGTACCTTCTAGAATGCAATCAAGAAGCCTAGAAGGGAAAATAAAAACCAGTTCAGAAAAAGCAAAACAATTAGAACAAGAATTAACAAAAAACGAAACAGATTTAGGCGGTCTAAAATCTCAATTAGATAAATTGAATGAGGACTTAAAAGCCCTAAACAAAGCAAATACTGACGGTTCTAAAAACGCAGAAATAGAAGCAAAATACAAAGATATAGATGCCCAAAATTTAAAAATAAAAACCGCTGAAGAAAAAAATAATAAATTGCAAAAAGAATTAAAAACTAAAAAAGATGAAATTATTAAAAAAACTTCTGAAAAGTCAAAATTAGATTTGAAGATTGCAGAATTGGAAACTAATAAAAATAAGGTTTCTGAACAAATTGATGCAAATAAAAAAGAATTAGAAGCATTGAATAAGGAATATGCAGACAGTGTTAAAAAGAGTGATGAATTAAGCAAATTACTTTCAAAAGAAAATGATAATTCACCAGCCAGTCAAGAAGCAGCGGCAAGACACATTTTAGAACTAAAAGACGACCTTGAAAATGAATTGGAAAACGCAAAATTAGATGATATAAGTGCTCCTACTGCTGAACAATCAAAGAAAATTTCTGAAATTTATGGTAAATATATTGAAAAAATTTCAAAAATAAATGATGCTTCACTAACAAGTGATTCTTTAGCATGA", "aa": "MKKQTRILISLGSIFGVAALMPMAASCTHTNNDELNKKIESLEKQLNEAKANSQKDQAQINDLMKQLQETKNNSKTIEEKEKLVKKLQEDLNKFQQERTAKYQTELNAANKAIVEIPNKYKELEQKNDDLKDQLENLSKELVPSRMQSRSLEGKIKTSSEKAKQLEQELTKNETDLGGLKSQLDKLNEDLKALNKANTDGSKNAEIEAKYKDIDAQNLKIKTAEEKNNKLQKELKTKKDEIIKKTSEKSKLDLKIAELETNKNKVSEQIDANKKELEALNKEYADSVKKSDELSKLLSKENDNSPASQEAAARHILELKDDLENELENAKLDDISAPTAEQSKKISEIYGKYIEKISKINDASLTSDSLA", "aa_hexdigest": "0a82dfbf4d04cfddeb13b972ab25d84e", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 41819.70550000005, "isoelectric_point": 6.0879335403442365 }, "id": "DFOKDJOJIG_881", "locus": "GCA013367855_00872" }, { "type": "rRNA", "sequence": "contig_1", "start": 498682, "stop": 498786, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8823529411764706, "score": 60.7, "evalue": 1.3e-09, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "CGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCCTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTT", "id": "DFOKDJOJIG_37", "locus": "GCA013367855_00873" }, { "type": "cds", "sequence": "contig_1", "start": 498995, "stop": 499147, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCCTCACTTCTATTATTATTTCTATGTATATAAATATGCATTAGGATATATCGTAGCAAATGTATTTTTCCAAAAATACAAACAAGAAGGCCAAGAAGCATTAAAAAACTATATAAATAAATTTTTAAGTGCCGGAGACATCGATTGA", "aa": "MVPHFYYYFYVYKYALGYIVANVFFQKYKQEGQEALKNYINKFLSAGDID", "aa_hexdigest": "ccab9e932d67397b07838c4ee1e3418c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6061.8657, "isoelectric_point": 7.928853797912598 }, "id": "DFOKDJOJIG_882", "locus": "GCA013367855_00874" }, { "type": "cds", "sequence": "contig_1", "start": 499304, "stop": 499588, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTTATATATTGATGAATTTTGATGGAACATTGGAATCAGTCAATACATTATGCCACGAAATGGGACATTCAATGCATTCATATTTTTCTAATAAATGCCAATCTCCACTTAGAAGCTCTTATCCAATATTTTTAGCAGAAATTGCTTCGATATTTAATGAATTATTACTAAATGATTATTTAATAAGCAAATCAAAAAATATTCAACAACAATTTTATTTACTTGATTCAAGCATTAATGATTTTATTGGCACAGTTTTGCGCCAAACGATGTGA", "aa": "MKKIYILMNFDGTLESVNTLCHEMGHSMHSYFSNKCQSPLRSSYPIFLAEIASIFNELLLNDYLISKSKNIQQQFYLLDSSINDFIGTVLRQTM", "aa_hexdigest": "6a78944ec2b0ad72b925be9724fc6b90", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10891.465399999988, "isoelectric_point": 6.241057777404785 }, "id": "DFOKDJOJIG_883", "locus": "GCA013367855_00875" }, { "type": "cds", "sequence": "contig_1", "start": 499787, "stop": 500269, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAACTTGATCATAAATTGTCAGATGATGTTGAAAATTATTTAAATGATACTGCAAGCGGTTCACCTGTACCTGAAGATATTTTTTCAATAATAAGCGATAGTGAAGTTGATTTTGGCAATGTTTTAATTAAAAATAAAAAAATAAAAATAACTGAAGGAAATAGAATAGCTTTATTAAAAAATAAAGATGAACAAGTTAGAAAAGATGTTTATTTTAATTATTTAAATGGATATTTAAAAAACAAACAATCATTAGCGAGATTGCTATATCAACATATTAAAGAAATATCAGTTAATGCTTTATATAGAAAATATAATTCATCTCTTGAATCTATTTTGTTGCAAGATCATGTTGATAAAAAGTTATTGAACATTATTTATGATGCAGTTCAAAAACATATGTATATATTTAAAAAATATAACAATGCCAAAAAACAATTTTTTAAAGCTAAATTCAATAAAAAATTAGAAAAATGA", "aa": "MQELDHKLSDDVENYLNDTASGSPVPEDIFSIISDSEVDFGNVLIKNKKIKITEGNRIALLKNKDEQVRKDVYFNYLNGYLKNKQSLARLLYQHIKEISVNALYRKYNSSLESILLQDHVDKKLLNIIYDAVQKHMYIFKKYNNAKKQFFKAKFNKKLEK", "aa_hexdigest": "8cd7040db18df7e979293f3522fcdff7", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18823.412900000003, "isoelectric_point": 9.33084774017334 }, "id": "DFOKDJOJIG_884", "locus": "GCA013367855_00876" }, { "type": "cds", "sequence": "contig_1", "start": 500318, "stop": 500581, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCAAAAATAATTGAAATAAAAGATAGCAAATACAATTCATTAGACCAATATGTTGAATATATTGAACTTTCAAAAAAATTCCTAATTTTAGGCAATAGAATTTCTAATTATTTGGATAATAAATTAAATATAAATATTGTTGATCCCACAATAAACAAAACAATTTCATTATTTGAAACAAAATCCCAAGAATATGCTAAAAAGTTAGGTTCAGAAGCCAACAGAATTTTTCAACATAAAGACAAAATCAAAACATGA", "aa": "MYSKIIEIKDSKYNSLDQYVEYIELSKKFLILGNRISNYLDNKLNINIVDPTINKTISLFETKSQEYAKKLGSEANRIFQHKDKIKT", "aa_hexdigest": "224e375b41de36fb76ea8eb75d953e69", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10248.67349999999, "isoelectric_point": 9.273406410217284 }, "id": "DFOKDJOJIG_885", "locus": "GCA013367855_00877" }, { "type": "cds", "sequence": "contig_1", "start": 500692, "stop": 501207, "strand": "-", "frame": 1, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0003999", "GO:0009116", "KEGG:K00759", "RefSeq:WP_012855642.1", "SO:0001217", "UniParc:UPI0001BC2388", "UniRef:UniRef100_D1J8F6", "UniRef:UniRef50_Q9RFQ2", "UniRef:UniRef90_D1J8F6" ], "nt": "ATGGATTTAAAAAAGTATATAAGAACTGTTGATGACTTTCCACAAAAAGGTGTCAAATTTAAAGATATTTCGCTTTTATTAGCTAATGGACAAGCGCTACATTATACAATTGATGAAATGGCTAAATTAGCAGAAGGTGCAGATATTATTGTGGGTCCTGATGCAAGAGGATTTTTATTCGGAACACCAGTAGCTGCTAAATTATCAAAACCATTTATTATGGTAAGAAAAAAAGGCAAATTGCCTGGTGAAGTTATAAGCTGCGATTATGGACTTGAATATGGTAGAAATATTTTAGAAATTCAAAAAGGTTTAGTTAAACCAGGTCAAAAAGCCGTCATAGTTGACGATGTTTTAGCAACCGGGGGTACATTGGAAGCAATTACTAAATTATTAACTGAACAAGGTGTAATTATCAGTAGAATAATTGTTTTAATGGAATTAGATGGCTTTAATTCTCATGAAAGATTGCCATACGATATAAAAAGTTTAATCTATATTAATAAAGGGGAATAA", "aa": "MDLKKYIRTVDDFPQKGVKFKDISLLLANGQALHYTIDEMAKLAEGADIIVGPDARGFLFGTPVAAKLSKPFIMVRKKGKLPGEVISCDYGLEYGRNILEIQKGLVKPGQKAVIVDDVLATGGTLEAITKLLTEQGVIISRIIVLMELDGFNSHERLPYDIKSLIYINKGE", "aa_hexdigest": "e1df95e8030d659ee05d448dda0526e2", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2388", "ncbi_nrp_id": "WP_012855642.1", "uniref100_id": "UniRef100_D1J8F6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2388", "RefSeq:WP_012855642.1", "UniRef:UniRef100_D1J8F6" ] }, "ips": { "uniref100_id": "UniRef100_D1J8F6", "uniref90_id": "UniRef90_D1J8F6" }, "psc": { "uniref90_id": "UniRef90_D1J8F6", "gene": "Apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_Q9RFQ2", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0003999", "GO:0009116" ] }, "pscc": { "uniref50_id": "UniRef50_Q9RFQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RFQ2" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "DFOKDJOJIG_886", "locus": "GCA013367855_00878" }, { "type": "cds", "sequence": "contig_1", "start": 501421, "stop": 501660, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAATTAATTTTTTTGCATTAGGTGGTTTGGATGAAAACGGCAAAAATTGTTATGTTTTGGAAATAAATAACAAACTATTTGTGATTAACTCAGGAACTAAAATTCCTATTAACTCGCATAACGGTATTGATACATTAATTTGCAATTACGAATTTTTAGAAAAACGTCAAAAAGATATAGAAGGATTATTTTTGACTGATGTTAGAAATGAAACGTTTTCTGCTATTCCTTGA", "aa": "MNKINFFALGGLDENGKNCYVLEINNKLFVINSGTKIPINSHNGIDTLICNYEFLEKRQKDIEGLFLTDVRNETFSAIP", "aa_hexdigest": "9227c53fcb884f46403e9711cc4e8abd", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8967.135899999994, "isoelectric_point": 5.212613105773924 }, "id": "DFOKDJOJIG_887", "locus": "GCA013367855_00879" }, { "type": "cds", "sequence": "contig_1", "start": 501667, "stop": 502041, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAAAACCTAAAAATATATACTAGTGCTTTTAACAAAATTGTTTTATTAGATAGAATTTCTAAATATAATATAGGTTCGAATACATTTGAAGTTAAAACAATAAATAAAAAAACAGCATTTGGCGGAGTAAATGTAACTCCTTTTGATCTAGCCGGAGGATTGCCTGGACAACTTGGGTTCAATTTCGAAACCCCAATTAGCAATATCTTGTTTATGACAAATTTTGTTGATGGTGATTTGGGTCCTTATGGTAAAACCGATTTGCAACAAATTCAACAAATAATTAATAACAAAAAGGAATTACAGCTTTTAATAATGGATAGTTCAAGAAGCGCTTTTCCTGGACGTTCAATTGAAAAACTTTGA", "aa": "MKIKNLKIYTSAFNKIVLLDRISKYNIGSNTFEVKTINKKTAFGGVNVTPFDLAGGLPGQLGFNFETPISNILFMTNFVDGDLGPYGKTDLQQIQQIINNKKELQLLIMDSSRSAFPGRSIEKL", "aa_hexdigest": "c211546d96dcb2aa95dca5bf55cfb2a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13803.850099999985, "isoelectric_point": 9.559452629089353 }, "id": "DFOKDJOJIG_888", "locus": "GCA013367855_00880" }, { "type": "cds", "sequence": "contig_1", "start": 502120, "stop": 503076, "strand": "+", "frame": 1, "gene": "rnjA", "product": "mRNA degradation ribonuclease J1/J2", "db_xrefs": [ "COG:COG0595", "COG:J", "SO:0001217", "UniRef:UniRef50_A0AAJ5NJJ8", "UniRef:UniRef90_D1J8F7" ], "nt": "ATGATAACTGCACATGAAATTTTAATACTTGCAAAACGTTATAACAGACCGGTTATAACTTATGGTTTAGCTTATTCTCAATTAATAAAACTTGTTGAAAAGGTAAATCCAAATTCTCAATTTCCAGAATTCGTTGAATATCAAACTGCAAATAATGTAAAAAATGCTGTTATTCTTGTAACGGGTGCAATAGATAGACTTTACACAAGATTTGCAAGAATTTCTAGCGATAATGATGTTTATTTAAAATTAAAAAATGATGATGCAGTCATTATAATTGCTCCACCAGTTAACGGATTGGAAGTAAATTACGCAATAACACTAGATGATATTGCTAGACATACTGCTAATTTAATTGAACTAGGCAATGATCAATATTATTCATGCAATCCTGCTAAATTAGATATTTTTAATATTATTAAAGCATTAAAACCTAAATTCTTTATACCAATTCAAGGTCTATACCGTTACCAAGTTGTAGCAACTCAAGTTGCTAGAAGCGCAGGTATGAACATTAGTAACTGCTTAGTGTTGCAAAACGGAAAGGTGGCTGAATTCAACGATACAAATTTAATTTCTCAAAAACATGGAATTAGAAATGTAGGTGATGTTATTATTGATGGCTATGGTATTGGCGATATTTCTCATGAAGTTATTAATGAAAGAGAAAACTTAGCAAGAGATGGTGTAATTGCTCTTAGCTCATTAATAGATTATAAAAAGAAGAAACCAATCAATGAACTTCAAATTTCTTCATATGGAATAATTACGAAAGATAATAAAGAAGTTATTCACAAAATAATTAATGATTTATTCAAAAAAGAATTTGAAAATAAACAAACAAAAGAAATAAATTTAAAGGAAATTCAAGAAAAATTAAGAAAATCAATTAAAAGAAAAATCGCCAAAACTATCGACAAAGAGCCAATGATTGTAATAACTTTATATGAAATTTAA", "aa": "MITAHEILILAKRYNRPVITYGLAYSQLIKLVEKVNPNSQFPEFVEYQTANNVKNAVILVTGAIDRLYTRFARISSDNDVYLKLKNDDAVIIIAPPVNGLEVNYAITLDDIARHTANLIELGNDQYYSCNPAKLDIFNIIKALKPKFFIPIQGLYRYQVVATQVARSAGMNISNCLVLQNGKVAEFNDTNLISQKHGIRNVGDVIIDGYGIGDISHEVINERENLARDGVIALSSLIDYKKKKPINELQISSYGIITKDNKEVIHKIINDLFKKEFENKQTKEINLKEIQEKLRKSIKRKIAKTIDKEPMIVITLYEI", "aa_hexdigest": "6d31e7ac111009a0ff6504458270a352", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnjA" ], "truncated": "5-prime", "pseudogene": { "start": 501820, "stop": 503076, "observations": { "mutation": [ 502039 ], "stop": [ 502039 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8F7", "query_cov": 1.0, "subject_cov": 0.5771324863883848, "identity": 1.0, "score": 571.0, "evalue": 3.86e-201, "gene_start": 1, "gene_end": 318, "reference_start": 234, "reference_end": 551, "reference_sequence": "MNKINFFALGGLDENGKNCYVLEINNKLFVINSGTKIPINSHNGIDTLICNYEFLEKRQKDIEGLFLTDVRNETFSAIPWLLMKIKNLKIYTSAFNKIVLLDRISKYNIGSNTFEVKTINKKTAFGGVNVTPFDLAGGLPGQLGFNFETPISNILFMTNFVDGDLGPYGKTDLQQIKQIINNKKELQLLIMDSSRSAFPGRSIEKLWVSRKIEYKFKETSDNQRIIVGLYDEDMITAHEILILAKRYNRPVITYGLAYSQLIKLVEKVNPNSQFPEFVEYQTANNVKNAVILVTGAIDRLYTRFARISSDNDVYLKLKNDDAVIIIAPPVNGLEVNYAITLDDIARHTANLIELGNDQYYSCNPAKLDIFNIIKALKPKFFIPIQGLYRYQVVATQVARSAGMNISNCLVLQNGKVAEFNDTNLISQKHGIRNVGDVIIDGYGIGDISHEVINERENLARDGVIALSSLIDYKKKKPINELQISSYGIITKDNKEVIHKIINDLFKKEFENKQTKEINLKEIQEKLRKSIKRKIAKTIDKEPMIVITLYEI" }, "paralog": false, "identity": 0.9952153110047847, "score": 767.0, "evalue": 6.01e-281, "description": "Internal stop codon at 502039. Nonsense mutation around 502039.", "psc": { "uniref90_id": "UniRef90_D1J8F7", "gene": "RnjA", "product": "mRNA degradation ribonuclease J1/J2", "uniref50_id": "UniRef50_A0AAJ5NJJ8", "cog_id": "COG0595", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8F7", "UniRef:UniRef50_A0AAJ5NJJ8", "COG:COG0595", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAJ5NJJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5NJJ8" ], "product": "Ribonuclease J" } }, "id": "DFOKDJOJIG_889", "locus": "GCA013367855_00881" }, { "type": "cds", "sequence": "contig_1", "start": 503576, "stop": 504121, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E31DEDA", "UniRef:UniRef90_UPI001E31DEDA" ], "nt": "GTGATTGGAACATTTATTAATTCAATATTAAATATCCCTGGAAAAAAACTTGGTTTTTCAATAGCTTACATAATAAGTATTGTTCTTTTAAGCTTATCTGTCATTTCATTTTTTATTAGTAACTCTTTCTTTAAAATGATATTCTCTCCAAAAAGCAAAAAGAACAAGGAAAAACTTCAAAATCAAAGCGATTTAAAAATAAAGGAAGAGGATATTGAAATAATTGATAACAAAGAAGTTGTAAATAGCAATGTTCAAATTTTAGAGACTAATTCCGACTCAAATAAAAATATAGACTCAGCACAAAATTCAAACCAAAATAACAATCTTGATTTAGATCCTTTTGATGACGAAAAAGAAATTGAAGAAGATTCAAGGCAAAAAGACAACAATGAGCTAGAATCAAAAGAAGCTATTGACTCACCAAGCAATGAAAATTCTATAGATCCTACACTACAAGAACCAGATAATTCAAATAAAAATAAAGAGGTTAAAAAAGAAGATAAGATCTATAAAATCATTGAAGACGAAGAAAATTTGTTCTAA", "aa": "MIGTFINSILNIPGKKLGFSIAYIISIVLLSLSVISFFISNSFFKMIFSPKSKKNKEKLQNQSDLKIKEEDIEIIDNKEVVNSNVQILETNSDSNKNIDSAQNSNQNNNLDLDPFDDEKEIEEDSRQKDNNELESKEAIDSPSNENSIDPTLQEPDNSNKNKEVKKEDKIYKIIEDEENLF", "aa_hexdigest": "0a5d0c938f3bd72386972bbae70c643b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 503438, "stop": 504121, "observations": { "mutation": [ 503516 ], "stop": [ 503516 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E31DEDA", "query_cov": 1.0, "subject_cov": 0.7973568281938326, "identity": 0.84, "score": 246.0, "evalue": 2.47e-80, "gene_start": 1, "gene_end": 181, "reference_start": 47, "reference_end": 227, "reference_sequence": "MGSVIYYTIHQVEHNSKNFFTKYFSSWFVDFSNSSNYLLPQKYTPGVIGTFINSILNIPGKKLGFSIAYIISIVLLSLSVISFFISNSFFKMIFSPKSKKNKEKLQNQSDLKIKEEDIEIIDNKEVVNSNIQILEANSNSNKNIDSAQNSNQNNNLDLDPFDDEKEIEEDSKQKDNNELEPKEAIDSPSNENSIDPTPQKPDNSNENKEVKKEDKIYKIIEDEENLF" }, "paralog": false, "identity": 0.8634361233480177, "score": 381.0, "evalue": 8.27e-136, "description": "Internal stop codon at 503516. Nonsense mutation around 503516.", "psc": { "uniref90_id": "UniRef90_UPI001E31DEDA", "uniref50_id": "UniRef50_UPI001E31DEDA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E31DEDA", "UniRef:UniRef50_UPI001E31DEDA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E31DEDA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E31DEDA" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20692.717800000006, "isoelectric_point": 4.484789848327637 }, "id": "DFOKDJOJIG_890", "locus": "GCA013367855_00882" }, { "type": "cds", "sequence": "contig_1", "start": 504469, "stop": 505419, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D7B81", "UniRef:UniRef90_UPI00215D7B81" ], "nt": "ATGAAAAACAAAAAAAGTAAAAAAATTGTGGTTGCACTTTCATTAACATTGATTGCATCCGCCATCACAATACCCGTTGTAATTTCATCTTTAATTATAAATAAGAATAATTGGGCTAACAAATACTATAGCCAAAGATCAGAGGCTAGTCATCTAGTTAATTTAATAGAAAAAAATAAGCATTTTTATTATTTTGCCTTAGAGGATCAAAATAATTTAAATAAGTTATTGAATAAAATTAATTTAACTAGCAAAAATAGTTCTGCCGATTTTAAAAACTCATATAATTTGCTTAACAAGACAAATCTTGATATCAACAAAAAGATAAATATTAACAAAAAAGAATTTGACAAAACTTATGCTGATATTTTGCTTTTAAGCCCACAGTTGCAAGATTTATTCAATAAAATTATTGATTTGAAATTGAACAACAATATAAAAGAAGATGTTGATGGACTATTACAAAGAGTTGAAAAAGATCTAAATAAATGTAGAAAAATAGATGAAAACATTAATCTTATTGATATTAAAGAAATATACGCACATAGTTCAAAAACAATCATTGATGTTTCAAATCAATTGAATTCATATTATAAATTAATTAGCAATAAAGATAAAACATATAAAGGCATTGTCTCAGAAATAAAAGATCTTAAAAAAGATTATTTCAACAAAATTGAAGATGGAGAAGAATATTACAAATTATATGGCAATGAAATAAACAATCTTAAAAAGAATTTTAAAAATTTTCAATCAGTCGAAGATTACAACAATTATATAAATTACGCCAATTCAAAAATAAAAGAAATTAAAGAAGAAATTGTTGAACACAGAAAGCTAAGCCAAAAGATAAAAAAAGATGGAAATGATATTCTAAATAATGATTATGATAAAAGTGCTTTAATAAGAAATTCATACTTCCAAGAACCGAAATCATTTTTATTAATGTGA", "aa": "MKNKKSKKIVVALSLTLIASAITIPVVISSLIINKNNWANKYYSQRSEASHLVNLIEKNKHFYYFALEDQNNLNKLLNKINLTSKNSSADFKNSYNLLNKTNLDINKKININKKEFDKTYADILLLSPQLQDLFNKIIDLKLNNNIKEDVDGLLQRVEKDLNKCRKIDENINLIDIKEIYAHSSKTIIDVSNQLNSYYKLISNKDKTYKGIVSEIKDLKKDYFNKIEDGEEYYKLYGNEINNLKKNFKNFQSVEDYNNYINYANSKIKEIKEEIVEHRKLSQKIKKDGNDILNNDYDKSALIRNSYFQEPKSFLLM", "aa_hexdigest": "af2f0fd0c9c6e8da8b8a5a392c11c4d2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00215D7B81", "query_cov": 0.9683544303797469, "subject_cov": 0.8116710875331565, "identity": 0.98, "score": 520.0, "evalue": 6.45e-184, "valid": true, "uniref50_id": "UniRef50_UPI00215D7B81" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D7B81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D7B81" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37036.90280000002, "isoelectric_point": 9.26186656951904 }, "id": "DFOKDJOJIG_891", "locus": "GCA013367855_00883" }, { "type": "cds", "sequence": "contig_1", "start": 505741, "stop": 506061, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA8CC", "UniRef:UniRef90_UPI00215DA8CC" ], "nt": "TTGTTTTATGACAAGGTTATTCAAGACCCAAAAACTAACGAAATTGATGAAGAAAAATTAATGAAATTAGATGACATCCATTTTGACCCAGAACAAGACATTAGATATTTACACTACCATTTTGGAAGTCTCATAACTCAAAAGTTTAATGAAATAATTAAGCATTATAACGAAAAAGCAAAAGCCTTATTAGAAAAAATAAAAAACAATAATAGTTACGAAACACAAAAAAATAGAATAGAAAAAATACTTGCTAAAAATATTACTTATGTAAATAATTTTAGAGGCGATTTCAATGATTACGAAAAATTCTATTGGTGA", "aa": "MFYDKVIQDPKTNEIDEEKLMKLDDIHFDPEQDIRYLHYHFGSLITQKFNEIIKHYNEKAKALLEKIKNNNSYETQKNRIEKILAKNITYVNNFRGDFNDYEKFYW", "aa_hexdigest": "0cdcf5230ca0953dd3e0188d2624b936", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 505741, "stop": 506205, "observations": { "mutation": [ 506059 ], "stop": [ 506059 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215DA8CC", "query_cov": 1.0, "subject_cov": 0.6883116883116883, "identity": 0.943, "score": 203.0, "evalue": 1.73e-65, "gene_start": 1, "gene_end": 106, "reference_start": 1, "reference_end": 106, "reference_sequence": "MFYDKVIQDPKTNEIDEEKLMKLDDIHFDPEQDIRYLHYHFGSLLTQKVNEIIRYYNKKAKALLEKIKNNNSYETQKNRIEKLLAKNITYVNNFRGDFNDYEKFYWWTYKFQKDSNGDIITSSNSEIPDFYKNEIEVAQYLHELDEILDNLKNI" }, "paralog": false, "identity": 0.948051948051948, "score": 306.0, "evalue": 1.26e-108, "description": "Internal stop codon at 506059. Nonsense mutation around 506059.", "psc": { "uniref90_id": "UniRef90_UPI00215DA8CC", "uniref50_id": "UniRef50_UPI00215DA8CC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215DA8CC", "UniRef:UniRef50_UPI00215DA8CC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA8CC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA8CC" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13043.553599999981, "isoelectric_point": 6.072530174255371 }, "id": "DFOKDJOJIG_892", "locus": "GCA013367855_00884" }, { "type": "cds", "sequence": "contig_1", "start": 506448, "stop": 506582, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGAGAAAGAAATTAATTGCTTTATCTGTGGCTTTTGGACTTTTAGCGCCTATTTCAGGAACTATTATGATTTCGTGCAAAAACGAAAAAAGTAATGTTGAATATCAAAAAGAACTGCAAAATAAATGA", "aa": "MKMRKKLIALSVAFGLLAPISGTIMISCKNEKSNVEYQKELQNK", "aa_hexdigest": "46b53058e4cd23101723706fb6a677aa", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4954.938200000001, "isoelectric_point": 9.807978248596193 }, "id": "DFOKDJOJIG_893", "locus": "GCA013367855_00885" }, { "type": "cds", "sequence": "contig_1", "start": 506709, "stop": 507254, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATTTTATTGCAAAAATTAATGAAACTACACAAGATATAAATGCACAAATTTCAAAATTAAAAAACGAAAACGGAAATACAAGCCCTGAAAACAATAGCCAAAATGATTTATTAAAAAAATTAAAAACAGAATTGGAAACGGCAACTAAAAAACTTAACATGCTACTTTCGGCAAATGAAAGTAAAAACATCTCAACAAAAGAAGAAGCTAAAAATATATTAAACGCCGCAAATAAAGCGTTAGAAAAATCAAATGAAAAAGAATATAAAAATCAAATTCAAAAAGTAAATGAAACTATCAATAAAATTGAAGAAGAAATATCAAATTTAGAAAAAGGCCCAAAATCAAAAGAATTTATTAAATTAAGAGAATTAAGAGAAAAGCTAATAAATTACTTAAATAGTAATTTGTCCGACTTTGCTTATAAAGATTTAAGAGCTGAAATTAAAGAATTAATTCAAAAACAAGAAAGCGTTTCAATTGTTTCAAATGAAAAAGAAATAAAGTCAGCAATTATGCAAATAAATGATATTTTTTGA", "aa": "MKNFIAKINETTQDINAQISKLKNENGNTSPENNSQNDLLKKLKTELETATKKLNMLLSANESKNISTKEEAKNILNAANKALEKSNEKEYKNQIQKVNETINKIEEEISNLEKGPKSKEFIKLRELREKLINYLNSNLSDFAYKDLRAEIKELIQKQESVSIVSNEKEIKSAIMQINDIF", "aa_hexdigest": "95ddcc5d185bfbc0feb665148606b0bc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20788.376000000007, "isoelectric_point": 8.535888099670409 }, "id": "DFOKDJOJIG_894", "locus": "GCA013367855_00886" }, { "type": "cds", "sequence": "contig_1", "start": 507369, "stop": 507902, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B330D9B", "UniRef:UniRef90_UPI000B330D9B" ], "nt": "TTGAATACCTCAATTTTTGAAAACATTGAAACAACAAATGAAAGAAATCTATTAAACAAATATATTAAAACAAATTTAACCAAAATTTCTGATGTAGAAAAACAAAATTATATTGAGGAAGTTAAAAAAACTATAAACTCTTTGTTAAACAAATATAATAATAATAATAATAATATAAATAACAATAATGATCAAATTTATGAATTAAATCGAAACATAGAACAATTAAAAAAATTAAACCAAATAGAAAATAATTATGTTATTGACAAAGTCAAACTACTAAATCTATTAAATAAAGCAAATACTATAAAAAATGAAGGTTCATTTGCTAAAAAAGCAATTTTACTAGAATCGTTAAGAGACAGCGTTTCAGAATTTCTTGAATTTTATTTACAATTAAGCCTTTATCTAAGAATCTTTTTTGATAAAAATAACGACATTATTAAATTATTACCAGCAGATTCACAAACAAAAATTGATGATTATCTAGACAAAATTAAAAAAATTACAGAAAAGAATAGTCAAGCCAAATGA", "aa": "MNTSIFENIETTNERNLLNKYIKTNLTKISDVEKQNYIEEVKKTINSLLNKYNNNNNNINNNNDQIYELNRNIEQLKKLNQIENNYVIDKVKLLNLLNKANTIKNEGSFAKKAILLESLRDSVSEFLEFYLQLSLYLRIFFDKNNDIIKLLPADSQTKIDDYLDKIKKITEKNSQAK", "aa_hexdigest": "b647d641467d8e86c2e1ea7a84aff7cb", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 507123, "stop": 508205, "observations": { "mutation": [ 507252, 507900 ], "stop": [ 507252, 507900 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000B330D9B", "query_cov": 1.0, "subject_cov": 0.4116279069767442, "identity": 0.9890000000000001, "score": 302.0, "evalue": 1.34e-99, "gene_start": 1, "gene_end": 177, "reference_start": 83, "reference_end": 258, "reference_sequence": "MSDFAYKDLRAEIKELIQKQESVSIVSNEKEIKSAIMQINDIFWIANIKKSILDKQFLYLENKPKSELDVKFLAAHDELSQALNTSIFENIETTNERNLLNKYIKTNLTKISDVEKQNYIEEVKKTINSLLNKYNNNNNINNNNDQIYELNRNIEQLKKLNQIENNYVIDKVKLLNLLNKANTIKNEGSFAKKAILLESLRDSVSEFLEFYLQLSLYLRIFFDKNNDIIKLLPADSQTKIDDYLDKIKKITEKNSQAKWSAPTFDEYISAFKAQEEIFKIISSDLKSADVNILEKLTNNLKEIKKQKKNDYDTIVTSYLKEKTEETITKNESIIAKKSNIESQNIIETFVLLQKLELEFAYINWTELNLITKIKNKLELLIKINKIDSNEEAKILKPWTDSLNSSKKPEIFELENILKELSKSLKELINK" }, "paralog": false, "identity": 0.9888888888888889, "score": 652.0, "evalue": 6.2e-240, "description": "Internal stop codon at 507252, 507900. Nonsense mutation around 507252, 507900.", "psc": { "uniref90_id": "UniRef90_UPI000B330D9B", "uniref50_id": "UniRef50_UPI000B330D9B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000B330D9B", "UniRef:UniRef50_UPI000B330D9B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000B330D9B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B330D9B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20841.438700000006, "isoelectric_point": 8.841468238830569 }, "id": "DFOKDJOJIG_895", "locus": "GCA013367855_00887" }, { "type": "cds", "sequence": "contig_1", "start": 508011, "stop": 508217, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAAATAATTTAAAAGAAATTAAAAAACAAAAGAAAAATGATTATGACACGATTGTTACCTCTTATTTAAAAGAAAAAACAGAAGAAACAATAACAAAAAATGAATCAATAATTGCTAAAAAATCTAATATTGAAAGTCAAAATATTATTGAAACGTTTGTTTTATTGCAAAAATTAGAACTTGAATTTGCTTACATTAATTGA", "aa": "MTNNLKEIKKQKKNDYDTIVTSYLKEKTEETITKNESIIAKKSNIESQNIIETFVLLQKLELEFAYIN", "aa_hexdigest": "575c0829f55184b1988357337f0dcdb7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8012.098699999996, "isoelectric_point": 6.252255058288575 }, "id": "DFOKDJOJIG_896", "locus": "GCA013367855_00888" }, { "type": "cds", "sequence": "contig_1", "start": 508436, "stop": 509227, "strand": "+", "frame": 2, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4", "UniRef:UniRef90_A0A454C8Z1" ], "nt": "GTGGGGTTAATTCCAAATAAGGAAAAAAAGCAAGATCATAAAGGTCTTGCTTTTTTTCTATCATTCATAAAAAAAATAAGTTTAAAAAAACGAAGAGCACAACACAAGCACAGCTCTTCATACATCTACATAAATTATACCACGTGCTCTTCGTTTTTTTTAAAAAACTTAAAAATTTTTGAAAGGAATTTTATGGGTGCTTATAGTGAATTTATGGTAATGCAGCAAGAGGCTAAATTAAACAACGATTATAGCCTTGTGGATGCATATGAAAGAGATTTAAATTGGAGGAGAAATTATAGTAAAAAAATATATGACAAATTAAGCGACATAGCTATAAGCAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATGGTTATTAAATCCTTTAAAAATAAAAAAATCAAAATCATTGATCTAATAAATTTAGGGTTTAAAAAAACTTCTATAAAATCAGCTATAAAAAGACTTATTGAAATAAAAGTTTTAGATGGTGAGTTTTATAAAAAATATAAACTTCTAAAATTAAAAAAAATAGTACTAAAAAACCCTAAGGAAAATTACTGGATTTTAAATGGCTATGATGCCTTAAAAATCTTTCTTCTTAATGGTTTAAGTAATGCTATTTTATATGCAATAAATAGGCATAAATCAAAGCAACTAAATGCTAAAAAATTGCATTGTAAAATAAACTCAAGAAAAGTGATTTTACAAAATGCATATTATGCAAAATTTAACTGA", "aa": "MGLIPNKEKKQDHKGLAFFLSFIKKISLKKRRAQHKHSSSYIYINYTTCSSFFLKNLKIFERNFMGAYSEFMVMQQEAKLNNDYSLVDAYERDLNWRRNYSKKIYDKLSDIAISKILKSKIVISESYYQKLKTNKVLLEVFMVIKSFKNKKIKIIDLINLGFKKTSIKSAIKRLIEIKVLDGEFYKKYKLLKLKKIVLKNPKENYWILNGYDALKIFLLNGLSNAILYAINRHKSKQLNAKKLHCKINSRKVILQNAYYAKFN", "aa_hexdigest": "9888806883b49ae0a181b8923c514e9d", "start_type": "GTG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A454C8Z1", "query_cov": 1.0, "subject_cov": 0.8092307692307692, "identity": 1.0, "score": 499.0, "evalue": 3.98e-177, "valid": true, "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_A0A9Q9F2D4" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4" ] }, "genes": [], "id": "DFOKDJOJIG_897", "locus": "GCA013367855_00889" }, { "type": "cds", "sequence": "contig_1", "start": 509249, "stop": 509413, "strand": "+", "frame": 2, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "RefSeq:WP_175972262.1", "SO:0001217", "UniParc:UPI00158EAE53", "UniRef:UniRef100_UPI00158C7902", "UniRef:UniRef50_A0A9Q9F2D4", "UniRef:UniRef90_A0A454C8Z1" ], "nt": "ATGAACAAAGCCATAGCCAATTATTTTGGAGTTGAATATTTGCAAATGTTTGGTATATTGCGTAGTCAAGAAAAAAAAGTTACAACATTAAAATTAGGAAATTTAAACATCAAATGTAATGTTTATAAATTTTCTACACAACGTTATTTGTTAATGCAAATTTAA", "aa": "MNKAIANYFGVEYLQMFGILRSQEKKVTTLKLGNLNIKCNVYKFSTQRYLLMQI", "aa_hexdigest": "cf685e3d1d55bbc71ebe10a3c2a6ac0f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158EAE53", "ncbi_nrp_id": "WP_175972262.1", "uniref100_id": "UniRef100_UPI00158C7902", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EAE53", "RefSeq:WP_175972262.1", "UniRef:UniRef100_UPI00158C7902" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C7902", "product": "MAGa4850 family ICE element protein", "uniref90_id": "UniRef90_A0A454C8Z1" }, "psc": { "uniref90_id": "UniRef90_A0A454C8Z1", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_A0A9Q9F2D4" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4" ] }, "genes": [], "id": "DFOKDJOJIG_898", "locus": "GCA013367855_00890" }, { "type": "cds", "sequence": "contig_1", "start": 509823, "stop": 509930, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E466C14", "UniRef:UniRef90_UPI001E466C14" ], "nt": "GTGGTAGATTACATTACAAGACCAGATAAATGCGTTGTATTTAAAGAAGATGAAAATAATTATGATGCTATGAAACTACTTCTTACTAAGGAAGATAAAATAAATTGA", "aa": "MVDYITRPDKCVVFKEDENNYDAMKLLLTKEDKIN", "aa_hexdigest": "4319b445e246145b5e30e86e9d399845", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 509823, "stop": 509991, "observations": { "insertion": [ 509973 ], "mutation": [ 509928 ], "stop": [ 509928 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E466C14", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 1.0, "score": 73.9, "evalue": 7.88e-17, "gene_start": 1, "gene_end": 35, "reference_start": 1, "reference_end": 35, "reference_sequence": "MVDYITRPDKCVVFKEDENNYDAMKLLLTKEDKINWYRQHLKINQNSKQSVFITY" }, "paralog": false, "identity": 0.9636363636363636, "score": 105.0, "evalue": 8.21e-32, "description": "Internal stop codon at 509928. Frameshift due to insertion around 509973. Nonsense mutation around 509928.", "psc": { "uniref90_id": "UniRef90_UPI001E466C14", "uniref50_id": "UniRef50_UPI001E466C14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E466C14", "UniRef:UniRef50_UPI001E466C14" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E466C14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E466C14" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4192.786300000001, "isoelectric_point": 4.716124534606934 }, "id": "DFOKDJOJIG_899", "locus": "GCA013367855_00891" }, { "type": "cds", "sequence": "contig_1", "start": 510459, "stop": 510863, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313EAF8", "UniRef:UniRef90_UPI001313EAF8" ], "nt": "TTGTGCCAAGAATTCATTAAATCTTCAAAAAGTATTTTAGATGAAGTTTTAAACAAAAAAAATATTTCATATTCTCGTATTTCTGATGAAAATAAGAAAAATATTGAAACAATAAAAAAATTCTTATTAAATTATGACAATGATTATTCAAAGCTCTTTAATTCTTATCAAAAACAAATTAATGATTTAAATAATTTAAACATCGAAGATAAATTTCTAAAACAAAGAATTTCAAAAATTATAAATAATGAAATGGATGATTTTCAAAAACAAGTTGGTAATAAGATAATCAAAACCTTACTAAATGCCTATAAGCAAAATAATAATGCATTTACTAGCCGAAAGAATTTCGCTTCATTTTTCAATGAATTTCTAAAAATATTTAGCCTAAATAGATCGGAATGA", "aa": "MCQEFIKSSKSILDEVLNKKNISYSRISDENKKNIETIKKFLLNYDNDYSKLFNSYQKQINDLNNLNIEDKFLKQRISKIINNEMDDFQKQVGNKIIKTLLNAYKQNNNAFTSRKNFASFFNEFLKIFSLNRSE", "aa_hexdigest": "4b09a3798400806a690ffc25e6783ff9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 510318, "stop": 510959, "observations": { "mutation": [ 510381, 510861 ], "stop": [ 510381, 510861 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001313EAF8", "query_cov": 1.0, "subject_cov": 0.6291079812206573, "identity": 0.985, "score": 247.0, "evalue": 1.67e-81, "gene_start": 1, "gene_end": 134, "reference_start": 48, "reference_end": 181, "reference_sequence": "MGLSIKYDEDYQKINALTKDIWNTRKQIVGIIANNFANSNEIISKSNLCQEFIKSSKSILDEVLNKKNISYSRISDKNKKNIETIKKFLLNYDNDYSKLFNSYQKQINDLNNLNIEDKFLKQRISKIINNEMDDFQKQVGNKIIKTLLNAYKQNNNAFTSRKNFASFFNEFLKIFSLNRSEWALRNLAEREFLAKDLKELELVEEFYRSKLVH" }, "paralog": false, "identity": 0.9812206572769953, "score": 406.0, "evalue": 8.09e-147, "description": "Internal stop codon at 510381, 510861. Nonsense mutation around 510381, 510861.", "psc": { "uniref90_id": "UniRef90_UPI001313EAF8", "uniref50_id": "UniRef50_UPI001313EAF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001313EAF8", "UniRef:UniRef50_UPI001313EAF8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001313EAF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313EAF8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15970.975899999985, "isoelectric_point": 9.44772891998291 }, "id": "DFOKDJOJIG_900", "locus": "GCA013367855_00892" }, { "type": "cds", "sequence": "contig_1", "start": 510967, "stop": 511119, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAACAAGAAAAATTATCAAAATCAAAAATTATAGGAAAGACACTTCTTTCAATAATTGTTAGTACATTTTTGTTTTTTGTTCTAACTTTTATTATTAATGTTTTTATGGGCCAGAATAAAAATCCTGGACATTTTAAACTTTTTTGA", "aa": "MEEQEKLSKSKIIGKTLLSIIVSTFLFFVLTFIINVFMGQNKNPGHFKLF", "aa_hexdigest": "ba2bb57e9f1e8664bda2b7f300a10a08", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5777.879399999999, "isoelectric_point": 9.699671363830568 }, "id": "DFOKDJOJIG_901", "locus": "GCA013367855_00893" }, { "type": "cds", "sequence": "contig_1", "start": 511477, "stop": 511644, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAATATTGAATTTATGGCACAACTTGATTATTCTAAAAAACCAAATTTAATAATTAGCGATCCAAAGAAAGAAATTATCAAATTTACAGGAGGCTTATTAACTGAAAACGATTACAAAATTTATGTAATTGATTTCTCTGACCCTAAATTATCTCTAAAATGA", "aa": "MPNIEFMAQLDYSKKPNLIISDPKKEIIKFTGGLLTENDYKIYVIDFSDPKLSLK", "aa_hexdigest": "9deac9918115d24a413a97288fdd0f20", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6364.380800000001, "isoelectric_point": 6.098903465270994 }, "id": "DFOKDJOJIG_902", "locus": "GCA013367855_00894" }, { "type": "cds", "sequence": "contig_1", "start": 511602, "stop": 511787, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCATTAACTGCCTTACCTCAATTTAAACTTTCAATAACATTATTTATAACTTCTAACGATTTATTAATTCTTTCTTCGAAAGTCAAATTCTTGAATTTTTCACCATCAACCGCTAAATCTCATGCATATGAAAGAGGGTTTCATTTTAGAGATAATTTAGGGTCAGAGAAATCAATTACATAA", "aa": "MPLTALPQFKLSITLFITSNDLLILSSKVKFLNFSPSTAKSHAYERGFHFRDNLGSEKSIT", "aa_hexdigest": "b7d7e4f1fd7336945595f2e5dd80b01d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6859.8555, "isoelectric_point": 9.696512413024902 }, "id": "DFOKDJOJIG_903", "locus": "GCA013367855_00895" }, { "type": "cds", "sequence": "contig_1", "start": 511789, "stop": 511929, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E578F32", "UniRef:UniRef90_UPI001E578F32" ], "nt": "ATGTGGATTAGCCAAGCTAAAAAAACATTAATTGTTATTACTAATTTCTTATTGCTATTAGGTATTGATGATAAAAATATTATTCATAAAAATAATTTTAATTTCGAAACAATAGCTTCATTATTAAATACTTCTCACTGA", "aa": "MWISQAKKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSH", "aa_hexdigest": "b1a13d795929f5c842693543b06bcbaf", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 511699, "stop": 511977, "observations": { "insertion": [ 511777 ], "deletion": [ 511808 ], "mutation": [ 511765, 511928, 511955 ], "stop": [ 511765, 511928, 511955 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E578F32", "query_cov": 0.8478260869565217, "subject_cov": 0.40625, "identity": 1.0, "score": 77.4, "evalue": 1.56e-17, "gene_start": 8, "gene_end": 46, "reference_start": 37, "reference_end": 75, "reference_sequence": "MTFEERINKSLEVINNVIESLNWGKALMAICGLAKPKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSHRENDKTISAWILYAKKSTWWQ" }, "paralog": false, "identity": 0.9347826086956522, "score": 147.0, "evalue": 7.22e-48, "description": "Internal stop codon at 511765, 511928, 511955. Frameshift due to insertion around 511777. Frameshift due to deletion around 511808. Nonsense mutation around 511765, 511928, 511955.", "psc": { "uniref90_id": "UniRef90_UPI001E578F32", "uniref50_id": "UniRef50_UPI001E578F32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E578F32", "UniRef:UniRef50_UPI001E578F32" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E578F32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E578F32" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5284.135999999999, "isoelectric_point": 8.231081581115724 }, "id": "DFOKDJOJIG_904", "locus": "GCA013367855_00896" }, { "type": "cds", "sequence": "contig_1", "start": 512032, "stop": 513000, "strand": "+", "frame": 1, "gene": null, "product": "type IV secretory system conjugative DNA transfer family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF33FFC", "UniRef:UniRef90_UPI000AF33FFC" ], "nt": "TTGTCAAAAACTCCTAAAGAAACATTTGGTGGAATATTAGCCGAAGCACAAAGTGTTTTAAATGTATTCAATAATGATGAAAATTTAAAGATGGTTTTAAGAGACACAGAAAGTTTCAATTTAGTTGATATAGTGTCCGATCCTAAACCTTTTGCTATATTTATACATTATCCTGACCATAAACCTACATCTCACTTTTTAGTAAGTATGTTGGTAGAACAAATTTATCAATCGTTAATTGAACAAGCAAACAAAAATGATAACCTAAAATTAGACAGAAAGGCTTTCTTTCTATTGGATGAATTCGGAAATTTACCTGTTGTTGTAAATTTTGATAATAAACTTACAATTTGTCGTTCAAGAAATATTTTCTTCATGCTGGTAGTACAAGATTACAATCAAATTAAGAAATATAACATATATGGAGAAGGAACAGCCCAAGTAATTAAAAACTCTTGCCAAGTAGTATATTTTTTAGCCTCTAATGATGAGCAAACATTAAACGATATTTCAAAAGCGCTCGGAACAAAAGAGGTTAAATCAAAAAGTTATACAACAAGTGATAACCATTCAAGTTCTACTGAAAGTGAAAAATCCAAACCTGTCATGGAAGTAGCGGAACTTAAATCTAAAAAACCTGATGATTTAATAATTATAAAAAATGGTTATAAACCTTTATTAATTAAAACCTTATTGGCTTATAAATATTTCCCAAATGATAATTATGTTTATACCAGAAACGAAAGTAAGCTACTAACTAATGCGGATTTTGAATTATTTGATATTAGAAGTTTGGAAAAACTTATTAAATTTGATAATTCTAAACAAAAAAAACAAAATAATAAAAACCCAAAAGTTTTAGATGATAACAATTTAACTTATTCAGAAGATGTTGAAGATCCTGCGGATTTATCCCAAGAACAAAAAATTCAAAGAATGAAGATGGTTTTTGGAAAATTAGATCTTTGA", "aa": "MSKTPKETFGGILAEAQSVLNVFNNDENLKMVLRDTESFNLVDIVSDPKPFAIFIHYPDHKPTSHFLVSMLVEQIYQSLIEQANKNDNLKLDRKAFFLLDEFGNLPVVVNFDNKLTICRSRNIFFMLVVQDYNQIKKYNIYGEGTAQVIKNSCQVVYFLASNDEQTLNDISKALGTKEVKSKSYTTSDNHSSSTESEKSKPVMEVAELKSKKPDDLIIIKNGYKPLLIKTLLAYKYFPNDNYVYTRNESKLLTNADFELFDIRSLEKLIKFDNSKQKKQNNKNPKVLDDNNLTYSEDVEDPADLSQEQKIQRMKMVFGKLDL", "aa_hexdigest": "4bef7560e91a67b66c035952680274f4", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 511801, "stop": 513267, "observations": { "mutation": [ 511927, 511954, 512002, 512998, 513100, 513151 ], "stop": [ 511927, 511954, 512002, 512998, 513100, 513151 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF33FFC", "query_cov": 1.0, "subject_cov": 0.6598360655737705, "identity": 0.972, "score": 602.0, "evalue": 3.4e-214, "gene_start": 1, "gene_end": 322, "reference_start": 78, "reference_end": 399, "reference_sequence": "QAKKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSHWENDKTISAWILYAKKRALDDNNYYWKKIYNNIEDLSKTPKETFGGILAEAQSVLNVFNNDENLKMVLRDTESFNLVDIVSDPKPFAIFIHYPDHKPTSHFLVSMLVEQIYQSLIEQANKNDNLKLDRKAFFLLDEFGNLPVVVNFDNKLTICRSRNIFFMLVVQDYNQIKKYNIYGEGTAQVIKNSCQVVYFLASNDEQTLNDISKALGTKEVKSKSYTTSDNHSSSTESEKSKPVMEVAELKSKKPDDLIIIKNGYKPLLIKTLLAYKYFPNDNYVYTRNESKLLTNADFELFDIRSLEKLIKLEKSKPKNQDNKNLKVLDDNNLTYSEDVEDPDDLSQEQKIQRMKMVFGKLDLWNNYINLKNDYKRTLIPHKKNQIKEQIEETLSLMWEQYVTNDLESDLLNILWSEAKERKIPNYHDLNVDILLYLLHPTEDISKLIEKSN" }, "paralog": false, "identity": 0.9713114754098361, "score": 926.0, "evalue": 0.0, "description": "Internal stop codon at 511927, 511954, 512002, 512998, 513100, 513151. Nonsense mutation around 511927, 511954, 512002, 512998, 513100, 513151.", "psc": { "uniref90_id": "UniRef90_UPI000AF33FFC", "product": "type IV secretory system conjugative DNA transfer family protein", "uniref50_id": "UniRef50_UPI000AF33FFC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF33FFC", "UniRef:UniRef50_UPI000AF33FFC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF33FFC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF33FFC" ], "product": "type IV secretory system conjugative DNA transfer family protein" } }, "id": "DFOKDJOJIG_905", "locus": "GCA013367855_00897" }, { "type": "cds", "sequence": "contig_1", "start": 513279, "stop": 513644, "strand": "+", "frame": 3, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6N2ZW67", "UniRef:UniRef90_UPI0034A1F568" ], "nt": "ATGAATATAAATAAAATAAAAGTTGTTGAATTATTTGGCGGAATAGGCGCTATTAGAAAAGCTCTAATTAGAACCATCAAACCTCCTAATTTTGAAATTATTGATTATGTCGAAAGTGATAAAAAAACAGTAAATGCTTATAATGCAATATATAACGATTTTTACAAACCTTTAGATATAAGAGATTATTCACTTCCTAATAATGTTAATGTTGATTTATTGTTTCACGGATCTCCTTGTCAAGATTTTTCGTGTGCAGGTAAAAATATCGGAGGAAGTAAAGAAAGTAAAAGTCGAAGTAGTTTATTGTGGGAAACAATTAGAATCATTAAAGAAGCTCAAATAAAACCCAAAATTGTTATCTGA", "aa": "MNINKIKVVELFGGIGAIRKALIRTIKPPNFEIIDYVESDKKTVNAYNAIYNDFYKPLDIRDYSLPNNVNVDLLFHGSPCQDFSCAGKNIGGSKESKSRSSLLWETIRIIKEAQIKPKIVI", "aa_hexdigest": "eaf6fad3de3dcb6e7243cee8bcd6a62c", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0034A1F568", "query_cov": 0.8925619834710744, "subject_cov": 0.9230769230769231, "identity": 0.981, "score": 218.0, "evalue": 7.63e-72, "valid": true, "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_A0A6N2ZW67" }, "pscc": { "uniref50_id": "UniRef50_A0A6N2ZW67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6N2ZW67" ], "product": "DNA (cytosine-5-)-methyltransferase" }, "genes": [], "id": "DFOKDJOJIG_906", "locus": "GCA013367855_00898" }, { "type": "cds", "sequence": "contig_1", "start": 514083, "stop": 514280, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00145.23" ], "nt": "ATGATTATAGATAATAAACCATATTATTTAAGGGTTATTACACCATTAGAAGCAATGCTATTAATGGGCTATGATCAGCAAGATTATGAAAGAATTAAGAATTTCTGTGATAGTCAAATTTATAAGTTTGCTGGAAATTCTATTGTTGTTAATGTATTGCAAGAAATAATTAAACACATTTTTAGACACAAACAATAA", "aa": "MIIDNKPYYLRVITPLEAMLLMGYDQQDYERIKNFCDSQIYKFAGNSIVVNVLQEIIKHIFRHKQ", "aa_hexdigest": "60bdb35f264425eba9a2a021602d885f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00145.23", "name": "DNA_methylase", "length": 56, "aa_cov": 0.8615384615384616, "hmm_cov": 0.9464285714285714, "evalue": 9.34828096425361e-11, "score": 37.29964828491211, "start": 6, "stop": 61 } ], "seq_stats": { "molecular_weight": 7807.056499999999, "isoelectric_point": 7.875280570983887 }, "id": "DFOKDJOJIG_907", "locus": "GCA013367855_00899" }, { "type": "cds", "sequence": "contig_1", "start": 514502, "stop": 514690, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6", "UniRef:UniRef90_UPI00158CD6B8" ], "nt": "ATGGAAGCAGATTTATGGGCGTTTGACATTAATAACATTTATGAAATGCAAAACGATTTTTATGATATTGAATTCGTGGGCATTGACGCCTATGGTAGAGCCTTCATCATAAGCAGAGAAGATGCAGAAAAATATTATGCAAAGTACTATTTAAAACAAAACAAGTTAGTTGAAAAAGGAGATAAATAA", "aa": "MEADLWAFDINNIYEMQNDFYDIEFVGIDAYGRAFIISREDAEKYYAKYYLKQNKLVEKGDK", "aa_hexdigest": "2b989bd0783900700549016f6cdfd84f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 514289, "stop": 514690, "observations": { "mutation": [ 514355, 514466 ], "stop": [ 514355, 514466 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158CD6B8", "query_cov": 1.0, "subject_cov": 0.46616541353383456, "identity": 0.9840000000000001, "score": 128.0, "evalue": 7.17e-37, "gene_start": 1, "gene_end": 62, "reference_start": 72, "reference_end": 133, "reference_sequence": "MTKDKYCAFCGKKIVGMYVKNFWSDFCSREFRAKSEKLQEDLNYWKFHEPQDVYPLNDAWELADKELRKYGMEADLWAFDINNIYEMQNDFYDIEFVGIDAYGRAFVISREDAEKYYAKYYLKQNKLVEKGDK" }, "paralog": false, "identity": 0.9548872180451128, "score": 269.0, "evalue": 4.87e-95, "description": "Internal stop codon at 514355, 514466. Nonsense mutation around 514355, 514466.", "psc": { "uniref90_id": "UniRef90_UPI00158CD6B8", "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158CD6B8", "UniRef:UniRef50_A0A6A8PZW6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7473.2735999999995, "isoelectric_point": 4.464725685119628 }, "id": "DFOKDJOJIG_908", "locus": "GCA013367855_00900" }, { "type": "cds", "sequence": "contig_1", "start": 515152, "stop": 515451, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAATGAAGAATATGTTTATGTAAATCCCTATTTAGAAATAGAACCAATTAATATTAAAGAAATTAAAGACGATTACATAGAAGCATTAAAAACTTTTGATTTAGATGACCTTTTAGAATCAAGAGATGAAAAATCATTAATTAATTTAGATCCTAACAAATCTTCTAAATCATCTTTATCTGATTTTGACAAGACCCCAACCGATCTCAAAGATTCCAAAGTAATCAACGAATATAACAATTCATCCCATACCCCCTCTGATACCCAATCAAAATCCCATTCATTAACAAAATAA", "aa": "MSNEEYVYVNPYLEIEPINIKEIKDDYIEALKTFDLDDLLESRDEKSLINLDPNKSSKSSLSDFDKTPTDLKDSKVINEYNNSSHTPSDTQSKSHSLTK", "aa_hexdigest": "a6561f80382dc09bd9ef82e293825631", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11374.318299999984, "isoelectric_point": 4.538900566101075 }, "id": "DFOKDJOJIG_909", "locus": "GCA013367855_00901" }, { "type": "cds", "sequence": "contig_1", "start": 515461, "stop": 515676, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAATTTTCAATCGTGATCAAATGGAACAAATCCACATTGGTTTAGTCCAAGGTCTTGATGTCACTCAATACACTCAACTAGACCCTCAAGGCCATCCAATATTCAATGATAATCAAATGACCGAAATCCGTAAGGGTCTAAAAAAAGGCCTTGATGTCTCTAAATATAATCAAATAGATCATGATGGATATCCGATATTTAATTGA", "aa": "MKKKIFNRDQMEQIHIGLVQGLDVTQYTQLDPQGHPIFNDNQMTEIRKGLKKGLDVSKYNQIDHDGYPIFN", "aa_hexdigest": "50c77cce3fde287027f172e412b761ee", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8302.352699999998, "isoelectric_point": 6.8166093826293945 }, "id": "DFOKDJOJIG_910", "locus": "GCA013367855_00902" }, { "type": "cds", "sequence": "contig_1", "start": 515683, "stop": 515775, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGTAATCCGTGAAGGTCTAGAACAAGGCCTTGACATCTCACAATACACCCAACTAGACCTCAAAGGCCATCCAATCTATGATTTTTGA", "aa": "MYVIREGLEQGLDISQYTQLDLKGHPIYDF", "aa_hexdigest": "f392ce09b464ff1f92d3e9b1614ffcd7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3542.9643000000005, "isoelectric_point": 4.427268791198731 }, "id": "DFOKDJOJIG_911", "locus": "GCA013367855_00903" }, { "type": "cds", "sequence": "contig_1", "start": 515779, "stop": 516261, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAATCCGCATTGGCCTAGAACATGGCCTTGACATCTCAATTTATGCAAAACAAAACAATTTTAATAGACCTATCTATGATTGTTTGCAAATGTCTGAAATCCGTGAAGGTCTAGAACAAGGCCTTGATATATCTCAATACAGCCAACTAGACCATAAAGGTCGCCCAATCTTTAATCGAGGTCAGATGTCTGAAATCCGTGAAGGTCTAGAACAAGGCCTTGATGTCTCACAATACACTCAACTAGACCTTCAAGGCCATCCAATATTCAATGGTTATCAAATGTATGCAATCCGTAAGGGTCTAGAACAAGGGCTTGACATCTCTCAATACACCCATTTGGCCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTCAAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCGGAATCCCAATCAAAATCCCATTCATTAACAAAATAA", "aa": "MEQIRIGLEHGLDISIYAKQNNFNRPIYDCLQMSEIREGLEQGLDISQYSQLDHKGRPIFNRGQMSEIREGLEQGLDVSQYTQLDLQGHPIFNGYQMYAIRKGLEQGLDISQYTHLASKSSLSDFDKTPTDLKDSKVINEYNKSSHTPSESQSKSHSLTK", "aa_hexdigest": "488f3e3c4bdf69e043f8da199ac57e64", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18269.103100000008, "isoelectric_point": 5.644987297058106 }, "id": "DFOKDJOJIG_912", "locus": "GCA013367855_00904" }, { "type": "cds", "sequence": "contig_1", "start": 516467, "stop": 516682, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAACAATTTTTGATAATGACCAAATGGAACAAATCTGCCTTGGTCTAGAACACGGCCTTGATATCTCTAAATATACTCAATTAAATCATAAAGGCCACCCTATATTTGATAGCTTCCAAATGAATGAAATCCGCGAAGGTCTAGAACACGGCCTTGATGTCACCCAATACACTCAACTAGACCCTCAAGGCCACCCCATCTATGATTGA", "aa": "MKKTIFDNDQMEQICLGLEHGLDISKYTQLNHKGHPIFDSFQMNEIREGLEHGLDVTQYTQLDPQGHPIYD", "aa_hexdigest": "010d3ad0acfd0d45522343f67a270a79", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8273.155999999995, "isoelectric_point": 4.917618751525878 }, "id": "DFOKDJOJIG_913", "locus": "GCA013367855_00905" }, { "type": "cds", "sequence": "contig_1", "start": 516785, "stop": 517276, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAATCCGCATTGGCATAGAACAAGACCTTGATATATCTCAATATAACAAACTAGACCCTCAAGGCCATCCAATTTTCGTTGGTAGCCAAATGTCTGAAATTCGTTATGGCCTAAAACAAGGCCTTGATGTCTCTAAATATACCCAACTAGACTCTCAAGGCCACCCTATATTTGACAGCTTTCAAATGGGTGAAATCCGTAAGGGTCTAAAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGATTTTTGCCAAATGGAACAAATCCGTGAAGGCATAAAACAAGGCCTTGACGTATCTAAACCCACTCAATTAGTCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCCCACTCACAACCTAATCAAAATAAAACCTCAGAATCAAAAAATATAAATAGATGCATATAG", "aa": "MEQIRIGIEQDLDISQYNKLDPQGHPIFVGSQMSEIRYGLKQGLDVSKYTQLDSQGHPIFDSFQMGEIRKGLKQGLDISKYTQLDPNGHPIYDFCQMEQIREGIKQGLDVSKPTQLVSKSSLSDFDKTPTDLQDSKVINEYNKSHSQPNQNKTSESKNINRCI", "aa_hexdigest": "838b3a0f7ae0a3d7c528f71ab940495b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18550.55810000001, "isoelectric_point": 5.783390235900878 }, "id": "DFOKDJOJIG_914", "locus": "GCA013367855_00906" }, { "type": "cds", "sequence": "contig_1", "start": 517267, "stop": 517575, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175972201.1", "SO:0001217", "UniParc:UPI00158A229D", "UniRef:UniRef100_UPI00158A229D", "UniRef:UniRef50_A0A6A8PZR1", "UniRef:UniRef90_A0A6A8PZR1" ], "nt": "ATGCATATAGATGAATTTATTGATAAATATCGCAGGAATTTAAAACAATCGGGTTTTAATAAAGACAATATAGACTTCTTTTTATATGGTTTAGAACTAGGATTAAATATAAAAATAAAATCCAGTAGAAATTATTACAACTTTAAAAAATCTATACATTATTCTTCAAATAATTCTCATCCCTCTTCCCTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCGGAATCCCTATCAAAATCCCATTCATTAACAAAATAA", "aa": "MHIDEFIDKYRRNLKQSGFNKDNIDFFLYGLELGLNIKIKSSRNYYNFKKSIHYSSNNSHPSSLSDFDKTPTDLQDSKVINEYNKSSHTPSESLSKSHSLTK", "aa_hexdigest": "046a545343a2a85e9a188a55c5994240", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158A229D", "ncbi_nrp_id": "WP_175972201.1", "uniref100_id": "UniRef100_UPI00158A229D", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158A229D", "RefSeq:WP_175972201.1", "UniRef:UniRef100_UPI00158A229D" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158A229D", "uniref90_id": "UniRef90_A0A6A8PZR1" }, "psc": { "uniref90_id": "UniRef90_A0A6A8PZR1", "uniref50_id": "UniRef50_A0A6A8PZR1" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZR1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11882.013199999987, "isoelectric_point": 9.098890495300292 }, "id": "DFOKDJOJIG_915", "locus": "GCA013367855_00907" }, { "type": "cds", "sequence": "contig_1", "start": 517585, "stop": 517896, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAATTTTCAATAGTGATCAAATGAATGAAATCCGCATTGGCCTAGAAGAAGGTCTAGACGTATCCAAATACATCCAATTATCCCCTCACGGTCGCCCAATCTATGATAGTTATCAAATGTATGCAATTCGTAAGGGTCTAAAACAAGACCTTGATATCTCTCAATATACCCAATTAAATCATAAAGGTCGCCCAATCTATGATGGTTATGAAATGTCTGTAATCCGTGAAGGTTTAAAAGCTGGCCTTGATGTCTCTCAATATAACCAACTAGACCCTCAAGGCCACCCAATTTTTGATTGA", "aa": "MKKKIFNSDQMNEIRIGLEEGLDVSKYIQLSPHGRPIYDSYQMYAIRKGLKQDLDISQYTQLNHKGRPIYDGYEMSVIREGLKAGLDVSQYNQLDPQGHPIFD", "aa_hexdigest": "126f5412d616ec80c3270f843a2a49d3", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11950.412999999993, "isoelectric_point": 6.2719213485717775 }, "id": "DFOKDJOJIG_916", "locus": "GCA013367855_00908" }, { "type": "cds", "sequence": "contig_1", "start": 517903, "stop": 518238, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCAATCCGTGAAGGTCTAGAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGCTGGTTATGAAATGTCTGTAATCCGTGAAGGTCTAGCCCAAGGCCTTGATATATCTCAATATAACCAACTAGACCCTCAAGGCCACCCAATTTTCGTTGGTAGCCAAATGCACGAAATTCGTAAGGGTCTAGAACAAGGCCTTGATATATCTCAATATACCCAATTAAATCATAAAGGCCATCCAATCTATAATGCTTACCAAATGCACGAAATTCGCATTGGCCTACTATTTGATTTTTTAGGATCATGA", "aa": "MYAIREGLEQGLDISKYTQLDPNGHPIYAGYEMSVIREGLAQGLDISQYNQLDPQGHPIFVGSQMHEIRKGLEQGLDISQYTQLNHKGHPIYNAYQMHEIRIGLLFDFLGS", "aa_hexdigest": "41904fd1a9cbc9c37b395101b508c202", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12619.068699999993, "isoelectric_point": 5.332713890075683 }, "id": "DFOKDJOJIG_917", "locus": "GCA013367855_00909" }, { "type": "cds", "sequence": "contig_1", "start": 518412, "stop": 518603, "strand": "+", "frame": 3, "gene": null, "product": "DUF1508 domain-containing protein", "db_xrefs": [ "RefSeq:WP_036439043.1", "SO:0001217", "UniParc:UPI000513025C", "UniRef:UniRef100_A0A454C8Y5", "UniRef:UniRef50_A0A9Q9F2Q3", "UniRef:UniRef90_A0A454C8Y5" ], "nt": "GTGTCAAAATACTTTTATAGATTAAGAGGGAGTCAATCTACAGGGAAGAATGCCAAAACATATTCGTTTGGCTCTGCGCAACAATACAATAAAGCATTAATAATGGCTCAAGATGCTATTTCAAAAAATCAATTTAAGGAGGTGCAAATAGAAAGAATACAAGTTATAAAAATTATTAAGCAAAATTCATAG", "aa": "MSKYFYRLRGSQSTGKNAKTYSFGSAQQYNKALIMAQDAISKNQFKEVQIERIQVIKIIKQNS", "aa_hexdigest": "bb61ae58264773743c7974dccf1b1a0d", "start_type": "GTG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000513025C", "ncbi_nrp_id": "WP_036439043.1", "uniref100_id": "UniRef100_A0A454C8Y5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513025C", "RefSeq:WP_036439043.1", "UniRef:UniRef100_A0A454C8Y5" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C8Y5", "uniref90_id": "UniRef90_A0A454C8Y5" }, "psc": { "uniref90_id": "UniRef90_A0A454C8Y5", "uniref50_id": "UniRef50_A0A9Q9F2Q3" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2Q3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2Q3" ], "product": "DUF1508 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_918", "locus": "GCA013367855_00910" }, { "type": "cds", "sequence": "contig_1", "start": 518617, "stop": 518727, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAACAATTATTCTTTTAATTATCATAATTAGACATTTGAAAAAACGCAATAAATTATTAGCAAATTCTAACCAAATTAATAGCGCTAAAGGAGTGATAAATAAATAA", "aa": "MITIILLIIIIRHLKKRNKLLANSNQINSAKGVINK", "aa_hexdigest": "eefd1c41d5c8ceca7425f556f70597b0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4096.028700000002, "isoelectric_point": 11.999967765808105 }, "id": "DFOKDJOJIG_919", "locus": "GCA013367855_00911" }, { "type": "cds", "sequence": "contig_1", "start": 518882, "stop": 519199, "strand": "+", "frame": 2, "gene": null, "product": "sugar-phosphate nucleotidyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313E05C", "UniRef:UniRef90_UPI001313E05C" ], "nt": "TTGGGCTATAAAGTAATATATTCGCATATTGATAATGGGCAAGATTTTTATAAATATGAGCCTATTGAAAATTATGATTTAATAATATCGAATCCTCCGTTTAGAAATAAAGCAAATATTATTAAAAGATTGCAAGAACTAAATAAACCGTTTGCTTTAATTTTTGGAGTTCAATGTTTTAATTCTGGCGGTTTTGTAAGCCAATTGCAAAAACTAAAAAATTTAGAGCTTGTGTTCTTAACAAAAAGAATTAAATTTCTTAAAAATTATAAACAAGACCTTAAAAACATTCCTCAGCCTACTTTTCATTCTCTATGA", "aa": "MGYKVIYSHIDNGQDFYKYEPIENYDLIISNPPFRNKANIIKRLQELNKPFALIFGVQCFNSGGFVSQLQKLKNLELVFLTKRIKFLKNYKQDLKNIPQPTFHSL", "aa_hexdigest": "3b68e2eb6f24447bacae676d47247993", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 518837, "stop": 519253, "observations": { "mutation": [ 519197 ], "stop": [ 519197 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001313E05C", "query_cov": 1.0, "subject_cov": 0.7553956834532374, "identity": 0.99, "score": 214.0, "evalue": 2.72e-70, "gene_start": 1, "gene_end": 105, "reference_start": 17, "reference_end": 121, "reference_sequence": "MPFDTQQSNFVIVLKSLGYKVIYSHIDNGQDFYKYEPIENYDLIISNPPFRNKANIIKRLQELNKPFALIFGVQCFNSGGFVSQLQKLKNLELVFLTKRIKFLKNYKQDLKNIPQPTFHSLWICSGITNKPLSILEGVK" }, "paralog": false, "identity": 0.9855072463768116, "score": 276.0, "evalue": 5.18e-97, "description": "Internal stop codon at 519197. Nonsense mutation around 519197.", "psc": { "uniref90_id": "UniRef90_UPI001313E05C", "product": "sugar-phosphate nucleotidyltransferase", "uniref50_id": "UniRef50_UPI001313E05C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001313E05C", "UniRef:UniRef50_UPI001313E05C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001313E05C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313E05C" ], "product": "sugar-phosphate nucleotidyltransferase" } }, "id": "DFOKDJOJIG_920", "locus": "GCA013367855_00912" }, { "type": "cds", "sequence": "contig_1", "start": 519778, "stop": 520035, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATAAAAATAATCCTAATCCTACCCCAACACCGGGTCCAGATTCGGGTTTAGTAAATCCAAATCCTTCAAACCCAACTCCTGCTCCTGCTCCAGTTCCAACCCCTGAAGTTAAACCAGAAGAAAAAGAAGGTTATGTTATTTATAAATCGACAAATGAAGATTATTTAAAAAAGAATAACACTTATTATTTTAGCAAATTGAGTTATAACCCCGTGGAATTGTCTGATGAGATTTATAATAGTCAAAAATGA", "aa": "MINKNNPNPTPTPGPDSGLVNPNPSNPTPAPAPVPTPEVKPEEKEGYVIYKSTNEDYLKKNNTYYFSKLSYNPVELSDEIYNSQK", "aa_hexdigest": "a91a8a19ef47d6e17cbb43d043924e66", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9484.356899999999, "isoelectric_point": 4.962578392028808 }, "id": "DFOKDJOJIG_921", "locus": "GCA013367855_00913" }, { "type": "cds", "sequence": "contig_1", "start": 520207, "stop": 520413, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGAAAGTGATGAAAACAAAGAAGGTATCAACCAAGGCGGTCGTTTTAAACCAAAAGATAATGAAAGTTATAATGAAGCCTTAATGAGATTTTTAAAAGAAATGGATGATTATTTAGATCCTGATATTGCATTTAAAGGTTTAACAATGGATGAAACTCATATAAATTATGTAACGGAAAATTTAGCTAAAGATCCTAAATGA", "aa": "MAESDENKEGINQGGRFKPKDNESYNEALMRFLKEMDDYLDPDIAFKGLTMDETHINYVTENLAKDPK", "aa_hexdigest": "e8234ce9e96894d500c75ddc58d5bb15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7872.6335999999965, "isoelectric_point": 4.491837882995605 }, "id": "DFOKDJOJIG_922", "locus": "GCA013367855_00914" }, { "type": "cds", "sequence": "contig_1", "start": 520450, "stop": 520560, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E37B992", "UniRef:UniRef90_UPI001E37B992" ], "nt": "ATGCCTTCTACTTTTATTAGAGACGAATTTAACCACCAATCAATTGAAGACATGGATGGATTTGTTAAAGAATTAATATCTGATTATGAAAATGCTATGTATGAAGGATGA", "aa": "MPSTFIRDEFNHQSIEDMDGFVKELISDYENAMYEG", "aa_hexdigest": "ca841bba6d4befa1bd0c027b5776d996", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 520423, "stop": 520681, "observations": { "insertion": [ 520600 ], "mutation": [ 520558 ], "stop": [ 520558, 520625 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E37B992", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 1.0, "score": 76.6, "evalue": 1.6e-17, "gene_start": 1, "gene_end": 36, "reference_start": 12, "reference_end": 47, "reference_sequence": "MNKNLFTHDSKMPSTFIRDEFNHQSIEDMDGFVKELISDYENAMYEGWTVENKANDWFVAPFHLDLILQWEINFHNKLMICILIKMV" }, "paralog": false, "identity": 0.9176470588235294, "score": 155.0, "evalue": 5.39e-51, "description": "Internal stop codon at 520558, 520625. Frameshift due to insertion around 520600. Nonsense mutation around 520558.", "psc": { "uniref90_id": "UniRef90_UPI001E37B992", "uniref50_id": "UniRef50_UPI001E37B992", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E37B992", "UniRef:UniRef50_UPI001E37B992" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E37B992", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E37B992" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4259.6153, "isoelectric_point": 4.0500284194946286 }, "id": "DFOKDJOJIG_923", "locus": "GCA013367855_00915" }, { "type": "cds", "sequence": "contig_1", "start": 520624, "stop": 520806, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAATAAATTTCCACAACAAACTTATGATATGTATACTGATAAAAATGGTCTAGATAAAACAACTCCTACTCCTGCTTATAATAACTACAATCCAGTAGCATTTAATTTCTTAGTAATGGAAAGATTTGACTTATCAGAAAAATTAAATTTTAAAGTTTATAAGAATTTAATTCAATGA", "aa": "MRNKFPQQTYDMYTDKNGLDKTTPTPAYNNYNPVAFNFLVMERFDLSEKLNFKVYKNLIQ", "aa_hexdigest": "35ddc5bf27f61669d63858c8582cfd40", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7217.132799999999, "isoelectric_point": 9.003735160827638 }, "id": "DFOKDJOJIG_924", "locus": "GCA013367855_00916" }, { "type": "cds", "sequence": "contig_1", "start": 521005, "stop": 521112, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E44B7D1", "UniRef:UniRef90_UPI001E44B7D1" ], "nt": "ATGAAGAGTTTAACAATAGGTGACAAAATTGACTTTGATTACACATATAGATTTGCTTATAAATTTTTATATGAAAAATTAGTGCCTACAATGGTGGCAAACGGTTGA", "aa": "MKSLTIGDKIDFDYTYRFAYKFLYEKLVPTMVANG", "aa_hexdigest": "8e5b417499fbc7ff4464986ab040ae0f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 521005, "stop": 521160, "observations": { "mutation": [ 521110 ], "stop": [ 521110 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E44B7D1", "query_cov": 1.0, "subject_cov": 0.660377358490566, "identity": 0.971, "score": 72.8, "evalue": 2.16e-16, "gene_start": 1, "gene_end": 35, "reference_start": 1, "reference_end": 35, "reference_sequence": "MKSLTMGDKIDFDYTYRFAYKFLYEKLVPTMVANGWNMPGEFDVLEKNMLDEK" }, "paralog": false, "identity": 0.8627450980392157, "score": 89.4, "evalue": 1.01e-25, "description": "Internal stop codon at 521110. Nonsense mutation around 521110.", "psc": { "uniref90_id": "UniRef90_UPI001E44B7D1", "uniref50_id": "UniRef50_UPI001E44B7D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E44B7D1", "UniRef:UniRef50_UPI001E44B7D1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E44B7D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E44B7D1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4139.787800000002, "isoelectric_point": 8.032776832580566 }, "id": "DFOKDJOJIG_925", "locus": "GCA013367855_00917" }, { "type": "cds", "sequence": "contig_1", "start": 521116, "stop": 521292, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATTGAATTTAATAATTTAGAAACGCTTATGCTAGACAGAAATAAAAAAGATTCATATATTTATGACTATATCTTTAAATATATAAATGTCATTTTTAAAGCTAAATATAAAGACATTGATGAAATCAAAAAACCAAATGGATATTTAGATTTAAATAATAAAGAACATTGA", "aa": "MPIEFNNLETLMLDRNKKDSYIYDYIFKYINVIFKAKYKDIDEIKKPNGYLDLNNKEH", "aa_hexdigest": "7d05961910cb80544d854c663838b2c6", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7121.105200000002, "isoelectric_point": 6.546112632751465 }, "id": "DFOKDJOJIG_926", "locus": "GCA013367855_00918" }, { "type": "cds", "sequence": "contig_1", "start": 521512, "stop": 521631, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATCGATGATAAAAGCTTTAGCTTTTTAGGAAATTTTAGTGAAACTATAATGAGGTCTATTTTTATAGCCCTTATTCCAGTTGCAATTATTGTATTAATAGGAATGATATTGTGA", "aa": "MKIDDKSFSFLGNFSETIMRSIFIALIPVAIIVLIGMIL", "aa_hexdigest": "d18ca949acc3f7ed5bd5f985436aca2a", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4358.317699999999, "isoelectric_point": 5.876663017272949 }, "id": "DFOKDJOJIG_927", "locus": "GCA013367855_00919" }, { "type": "cds", "sequence": "contig_1", "start": 521876, "stop": 522100, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E52DBAB", "UniRef:UniRef90_UPI001E52DBAB" ], "nt": "ATGAAATTTTTACAGGCGATAATAAATCCTTTAATAAATGTAATCGCAGCGCCTATTAGGCTTATGTGGTTTGTACCTCTTGCTGCTATTGGATTAATAGGATTTACTATATTTAATCTTTTGTTTTTCTTGTTTAAATTGTTTAGCCTCGATTTAAACAACTATTTAATGTTTAATTTATTACCAGGCCAAAGTTATAGCCAATTTTCTGTAGCAAGGCCTTGA", "aa": "MKFLQAIINPLINVIAAPIRLMWFVPLAAIGLIGFTIFNLLFFLFKLFSLDLNNYLMFNLLPGQSYSQFSVARP", "aa_hexdigest": "d1e23a59454a415dd491de6565c094cc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 521876, "stop": 522188, "observations": { "insertion": [ 522143 ], "mutation": [ 522098, 522131 ], "stop": [ 522098, 522131 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E52DBAB", "query_cov": 1.0, "subject_cov": 0.7184466019417476, "identity": 0.973, "score": 135.0, "evalue": 8.74e-40, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MGFLKAIINPLINVIAAPIRLMWFVPLAAIGLIGFTIFNLLFFLFKLFSLDLNNYLMFNLLPGQSYSQFSVARPWYQFLIISFILWLINFLCLLLGIQKNLMD" }, "paralog": false, "identity": 0.941747572815534, "score": 182.0, "evalue": 3.87e-61, "description": "Internal stop codon at 522098, 522131. Frameshift due to insertion around 522143. Nonsense mutation around 522098, 522131.", "psc": { "uniref90_id": "UniRef90_UPI001E52DBAB", "uniref50_id": "UniRef50_UPI001E52DBAB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E52DBAB", "UniRef:UniRef50_UPI001E52DBAB" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E52DBAB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E52DBAB" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8468.174799999993, "isoelectric_point": 9.991971015930176 }, "id": "DFOKDJOJIG_928", "locus": "GCA013367855_00920" }, { "type": "cds", "sequence": "contig_1", "start": 522793, "stop": 523323, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTATTCTTTCGATTTGGCATTATTAAAATAATTCCTAATAAAAAAGTAGGTATTCAAAATGCAAAGAAAATTAATTTCACATTTATTAATTGGCTAAAACCAAAACTAATAAAAAATGATAATAATAGAATTACAACAAATTCAATAAGATCTTTTGTTTTGAAAGTTTTTCAAATTTTACTACTTCTATGTTTTATTCTACTTGGCTGTAATTGCGTCATATTACTCCTTATACTTTGCTATTTTAGAAAACTTGCAACTTTATATGCTGCACCTACATTCGTGCCCTTTAATGCTGCATCCTTTACTGTTTTGTATGCTCCTGCCCCTGATCTTGCTCCAGACATAGCAAGTCTTGCAGATTGGCCACCAGAAACTTCACTTCCAACAAAAGCAATTGCAATTTCTGATAATTTTTTGAAAGCAAATGCAGAGCCAATTACCAAGAAACAAGTTGATAATAATGCTAAAACGGCTTTGATAGTAGTAAAGTTTGAGAATGAAGAAAAGGCTCCTATCATATAA", "aa": "MQLFFRFGIIKIIPNKKVGIQNAKKINFTFINWLKPKLIKNDNNRITTNSIRSFVLKVFQILLLLCFILLGCNCVILLLILCYFRKLATLYAAPTFVPFNAASFTVLYAPAPDLAPDIASLADWPPETSLPTKAIAISDNFLKANAEPITKKQVDNNAKTALIVVKFENEEKAPII", "aa_hexdigest": "602567f04168dc9dd7e9ce7b340f50b7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19755.3747, "isoelectric_point": 9.608642005920409 }, "id": "DFOKDJOJIG_929", "locus": "GCA013367855_00921" }, { "type": "cds", "sequence": "contig_1", "start": 523415, "stop": 523552, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTAAATCCTTATTCATGTTTAGTTAATGAATATGTAGTCAAAAATAAAAATTCAAAAATAAGTTTTTATAATCCAAGAAATGATAAAGAAAACTATTTAGCGGTATTTAAACTAAATGGTCTTAATATTTGA", "aa": "MDLNPYSCLVNEYVVKNKNSKISFYNPRNDKENYLAVFKLNGLNI", "aa_hexdigest": "e4028ef62a76857cd24bf8df97f42e76", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5283.9867, "isoelectric_point": 8.852041053771973 }, "id": "DFOKDJOJIG_930", "locus": "GCA013367855_00922" }, { "type": "cds", "sequence": "contig_1", "start": 523889, "stop": 524125, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATAAAGCAAGACTTCTAACATTTCTTAATAAACTTAATATGTTTGGTATTGATAGCCAAAACATACAAGAATATTGCAATGATTTAGATGCTAAATTTGGAGTTGAAAATTCATTAGATAACTTATTTATTTATAATCAAATTATTATTAAAGATAAATATTTACAAATCAATGATAATTACGTAAAAATCAATTGTTTAAACAAATTGCCATTTGAATTGTCTGATAAATGA", "aa": "MHKARLLTFLNKLNMFGIDSQNIQEYCNDLDAKFGVENSLDNLFIYNQIIIKDKYLQINDNYVKINCLNKLPFELSDK", "aa_hexdigest": "bb48840de9856d6d1d99c6de438389c9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9218.523299999993, "isoelectric_point": 5.646124076843263 }, "id": "DFOKDJOJIG_931", "locus": "GCA013367855_00923" }, { "type": "cds", "sequence": "contig_1", "start": 524219, "stop": 524941, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01656.29", "PFAM:PF01935.23", "PFAM:PF10412.15" ], "nt": "ATGGCATCTATTGATACTACTAACGCAACAGGATCGGTGTCAGCAAACTTGGTTGGTTCTATTGATGAACAAGCGACAATGGCGATGATGTATCAAGTAAATAAAGATGAATACAAACTATTTGATAGTAATTTCTTCTTTGTATCAAAAGCCACTGACCTTGCTGAATTAAATAGACAGCATAGAATTGCTAGAAATATAGCAAGTAAAAAGAATATAAGCTTAAATGACTTAAAATATAGACAGCTTTTGGGTTTATCTGAGGCTTGCAATTATCCTTTTGAAAGATTAGAAAGAGAATATTATCAAATTACATCTATTAATTTAGCTTTGGGATGGCCTTTTGAAACCGAAAACCACAATGATTCAAATAATTTACTTCTAGGTTACTCAGAAGCCACAAGAAGTCCTCTAAGTTTTAGATTATTTGATATGTATCATTCAAATAGAACTAATTTTAATATGTTTATAAATGGTACTAGTGGAAAAGGTAAAACAACTTTTACTATGAAGTTATTAACCAGCATTTTAGCAGCAAATAATAAAGTTGTTGTTATTGACCCTCAAGGAGAATATAGAGAATTAGCTCAAAAACTTAATGGACAAATTATTGATTTGGGAGCAGGTAAAAACACCATAATCAATCCTCTTCAAATCCGTAATTCATTAAAGGATGCAACGGGTAATGATACATCTTCACTAATTAACAACCATTTGTCGTGA", "aa": "MASIDTTNATGSVSANLVGSIDEQATMAMMYQVNKDEYKLFDSNFFFVSKATDLAELNRQHRIARNIASKKNISLNDLKYRQLLGLSEACNYPFERLEREYYQITSINLALGWPFETENHNDSNNLLLGYSEATRSPLSFRLFDMYHSNRTNFNMFINGTSGKGKTTFTMKLLTSILAANNKVVVIDPQGEYRELAQKLNGQIIDLGAGKNTIINPLQIRNSLKDATGNDTSSLINNHLS", "aa_hexdigest": "cf0d37f42301b4c3b8415fe707399eb9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01656.29", "name": "CbiA", "length": 42, "aa_cov": 0.175, "hmm_cov": 1.0, "evalue": 2.9843715594778944e-07, "score": 26.120105743408203, "start": 155, "stop": 196 }, { "id": "PF01935.23", "name": "DUF87", "length": 68, "aa_cov": 0.26666666666666666, "hmm_cov": 0.9705882352941176, "evalue": 2.462838157755879e-10, "score": 36.4718132019043, "start": 157, "stop": 220 }, { "id": "PF10412.15", "name": "TrwB_AAD_bind", "length": 72, "aa_cov": 0.3, "hmm_cov": 0.875, "evalue": 1.5735730484289072e-10, "score": 36.0557746887207, "start": 149, "stop": 220 } ], "seq_stats": { "molecular_weight": 26953.050900000024, "isoelectric_point": 6.912837791442871 }, "id": "DFOKDJOJIG_932", "locus": "GCA013367855_00924" }, { "type": "cds", "sequence": "contig_1", "start": 524993, "stop": 525370, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTCAAAACGTAATAAAAGAATTTTATGAAGATAGAGGAATATATAAACTTGCTTCTATTGAGCAATTAAAGGCCGTAGAATGGCCAACAATAAGTGAGTTGATAAAATATATGTCCAACTATAAAACGCTTCATTTAAACAATCCTGAAGCAAAAAGAGCAATATTAGTTGAACTTTGTGAATCGTTTAGCTTTTTGTTTGAAAATAATGGAAGATATCAAAACTTATTTAATGGACATACTAATATAAAACTTGATAATGATTTTGTAGTATTTAATACGCAAAACTTAATAAATGGAGCGGATAATATCGGCGCTAAATTAGGAACAATGTGCTTATTAAACTTTGTTAGTGAAATAGTATTTAATAATTGA", "aa": "MLQNVIKEFYEDRGIYKLASIEQLKAVEWPTISELIKYMSNYKTLHLNNPEAKRAILVELCESFSFLFENNGRYQNLFNGHTNIKLDNDFVVFNTQNLINGADNIGAKLGTMCLLNFVSEIVFNN", "aa_hexdigest": "b7282ea7acc765500ab8863ad00101c8", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14430.339799999983, "isoelectric_point": 5.328053092956543 }, "id": "DFOKDJOJIG_933", "locus": "GCA013367855_00925" }, { "type": "cds", "sequence": "contig_1", "start": 525383, "stop": 525874, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F9", "UniRef:UniRef90_A0A454C9F9" ], "nt": "ATGTTAAAGAGAAAATATTTGCAAGAACATAACATTAAGTTAATGGATGCAAAACAAGTAGAAGATACTATTAGATTTTGTGCCGTCATCGTAGATGAAGCCCATTTATACATCGACCCTGATAATCCTGCCATTTTAAAATATATGGTTCAAATGACAAAAACGGTTAGAAAATTCAATGCAGGTATGATATTTACTACCCAAAATCCTGGTGATTTTACTATTAATAATGCCACAAGTGATGATGCATCTAGAATTATTCAAAACTGTCAATATTCTGTATTCTTTGGCCTAAAAGACCAAGATATTGACAAAGTGCAACAATTATTTAAAAACTCTAATCAATTATTACAAAGTGAAATTAGATATTTAGTAAATGCAAGATATGGAAAATGTTTATTTTCAATAAGTTCAAATAAAAGAATTAGAGTTGAAATTTATTACAACAAATTAGAACAAGAATTATTCTTCAAGTTCTTAAATAAAGAATGA", "aa": "MLKRKYLQEHNIKLMDAKQVEDTIRFCAVIVDEAHLYIDPDNPAILKYMVQMTKTVRKFNAGMIFTTQNPGDFTINNATSDDASRIIQNCQYSVFFGLKDQDIDKVQQLFKNSNQLLQSEIRYLVNARYGKCLFSISSNKRIRVEIYYNKLEQELFFKFLNKE", "aa_hexdigest": "dc888545ecc1107b7cb41b23ec2f7010", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 525083, "stop": 525874, "observations": { "mutation": [ 525368 ], "stop": [ 525368 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9F9", "query_cov": 1.0, "subject_cov": 0.5174603174603175, "identity": 0.9940000000000001, "score": 323.0, "evalue": 8.79e-110, "gene_start": 1, "gene_end": 163, "reference_start": 151, "reference_end": 313, "reference_sequence": "MSWLESFFKVLINIEDHKWILLQNVIKEFYEDRGIYKLASIEQLKAVEWPTISELIKYMSNYKTLHLNNPEAKRAILVELCESFSFLFENNGRYQNLFNGHTNIKLDNDFVVFNTQNLINGADNIGAKLGTMCLLNFVSEIVFNNWTHNEMLKRKYLQEHNIKLMDAKQVEDTIRFCAVIVDEAHLYIDPDNPAILKYMVQMTKTVRKFNAGMIFTTQNPGDFTVNNATSDDASRIIQNCQYSVFFGLKDQDIDKVQQLFKNSNQLLQSEIRYLVNARYGKCLFSISSNKRIRVEIYYNKLEQELFFKFLNKEWI" }, "paralog": false, "identity": 0.9923954372623575, "score": 526.0, "evalue": 3.22e-192, "description": "Internal stop codon at 525368. Nonsense mutation around 525368.", "psc": { "uniref90_id": "UniRef90_A0A454C9F9", "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A454C9F9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9F9", "UniRef:UniRef50_A0A454C9F9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9F9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F9" ], "product": "ATP-binding protein" } }, "id": "DFOKDJOJIG_934", "locus": "GCA013367855_00926" }, { "type": "cds", "sequence": "contig_1", "start": 525932, "stop": 526198, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAACTAATAAAAACGAATTAGATCAAGATTTTGATAATTTAGATAATAATGAAATTTTATCAAATGACAATCAAACTCAAAATGAAGATGACTTTATAGAAAATATTAATTTAACAAGTGATAATCAAATAAATAACATTATGAATTCTATCACTAGTTCCCAACCTAAAAAGCAAAAGGAAAAAAAGCAACAAAACAATTTTGAAGCACAATTTTCAGGACGATTTACTGATCCTGAAGATGTAGGAATGTTTTATCGTTGA", "aa": "MKTNKNELDQDFDNLDNNEILSNDNQTQNEDDFIENINLTSDNQINNIMNSITSSQPKKQKEKKQQNNFEAQFSGRFTDPEDVGMFYR", "aa_hexdigest": "99fc51e43057f7e6bac09ce1ee956bb5", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10338.974399999996, "isoelectric_point": 4.283920860290528 }, "id": "DFOKDJOJIG_935", "locus": "GCA013367855_00927" }, { "type": "cds", "sequence": "contig_1", "start": 526403, "stop": 526567, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00138A25BD", "UniRef:UniRef90_UPI00138A25BD" ], "nt": "ATGTCTATTATTAATTTAAAACTTAATATGATTATTAATGCTTTATTTAAAGATAATGGAGATTTAGAAAAACAAATTGATAATCCCGGGGCTGATATATTAGCAGAATCTAAGTTCTTTGAACGCACTATAAGAGGTGCTGTTAATAAATTAACTAATGATTGA", "aa": "MSIINLKLNMIINALFKDNGDLEKQIDNPGADILAESKFFERTIRGAVNKLTND", "aa_hexdigest": "6426191af12777e3dadae3b37578ec71", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 526343, "stop": 526576, "observations": { "mutation": [ 526565 ], "stop": [ 526565 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00138A25BD", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 110.0, "evalue": 1.14e-30, "gene_start": 1, "gene_end": 54, "reference_start": 22, "reference_end": 75, "reference_sequence": "MSALSPYQLQINRRFRSMDIEMSIINLKLNMIINALFKDNGDLEKQIDNPGADILAESKFFERTIRGAVNKLTNDWTKNGK" }, "paralog": false, "identity": 0.987012987012987, "score": 149.0, "evalue": 2.08e-48, "description": "Internal stop codon at 526565. Nonsense mutation around 526565.", "psc": { "uniref90_id": "UniRef90_UPI00138A25BD", "uniref50_id": "UniRef50_UPI00138A25BD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00138A25BD", "UniRef:UniRef50_UPI00138A25BD" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00138A25BD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00138A25BD" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6063.910899999999, "isoelectric_point": 5.175554084777831 }, "id": "DFOKDJOJIG_936", "locus": "GCA013367855_00928" }, { "type": "cds", "sequence": "contig_1", "start": 526844, "stop": 527161, "strand": "+", "frame": 2, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA66", "UniRef:UniRef90_A0A454CA66" ], "nt": "TTGCCCTTTGTTATTGCACTATTTGAACAAGCAAATAAAACCAATTTTATCGAGATTACTTGTTTTAATAAATTGGCAGAACATGTTATGAAATATGTTAAAAAAGGAGATTTATTAGAAGTTGTTGGCAAATTACACAATGCTAAATTTAGTTCAAAGAATAACGAAACTATTTATAAAACAGAAATAATTGCTAAAAATATTACTTTTTTGACAAAGTCAAAATCAAATATTCAACCCATTTCAAAAGAAACCAACTCAGAAAATAATGAAAAAGACATTTTTGGAATAGATTTTTTTGAAGATGAAGAAAATTAA", "aa": "MPFVIALFEQANKTNFIEITCFNKLAEHVMKYVKKGDLLEVVGKLHNAKFSSKNNETIYKTEIIAKNITFLTKSKSNIQPISKETNSENNEKDIFGIDFFEDEEN", "aa_hexdigest": "f16d7948cf267e4824cd45f63ab496fc", "start_type": "TTG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A454CA66", "query_cov": 1.0, "subject_cov": 0.8076923076923077, "identity": 0.99, "score": 201.0, "evalue": 6.25e-65, "valid": true, "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_A0A454CA66" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA66", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA66" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "DFOKDJOJIG_937", "locus": "GCA013367855_00929" }, { "type": "cds", "sequence": "contig_1", "start": 527148, "stop": 527342, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EDE39", "UniRef:UniRef90_UPI001E2EDE39" ], "nt": "ATGAAGAAAATTAAAAAAACCTTATTGATAGCCGGAGGAATATTTAGTATTGCTTCGGCTTCTCTATTTTCTTTGAGTTTTTCTAGAAATGAACATTATTCAAGTTATAAATATGAAGAAATTGACAAAGACCAATATTCTTTTTTAAACAACAAAAATTTATTAAAAAGTTTTTTTACAAATTTGACATATTGA", "aa": "MKKIKKTLLIAGGIFSIASASLFSLSFSRNEHYSSYKYEEIDKDQYSFLNNKNLLKSFFTNLTY", "aa_hexdigest": "3c30da2aa9a547cf19278edfa2c4f5b6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 527148, "stop": 527442, "observations": { "insertion": [ 527409 ], "mutation": [ 527340 ], "stop": [ 527340 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2EDE39", "query_cov": 1.0, "subject_cov": 0.6530612244897959, "identity": 1.0, "score": 123.0, "evalue": 1.85e-35, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MKKIKKTLLIAGGIFSIASASLFSLSFSRNEHYSSYKYEEIDKDQYSFLNNKNLLKSFFTNLTYKKNSRQDSFIFNPKSYSGNPLYSIKDLLQTKMIT" }, "paralog": false, "identity": 0.9278350515463918, "score": 171.0, "evalue": 1.17e-56, "description": "Internal stop codon at 527340. Frameshift due to insertion around 527409. Nonsense mutation around 527340.", "psc": { "uniref90_id": "UniRef90_UPI001E2EDE39", "uniref50_id": "UniRef50_UPI001E2EDE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2EDE39", "UniRef:UniRef50_UPI001E2EDE39" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2EDE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EDE39" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7455.452299999998, "isoelectric_point": 9.477255439758302 }, "id": "DFOKDJOJIG_938", "locus": "GCA013367855_00930" }, { "type": "cds", "sequence": "contig_1", "start": 527583, "stop": 527732, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGGCTACAAAAAATATATCAACGACTTAACTTTTTATCACGGCGATGGTAATGAGTGGTGTGATCAAGATGGGTGCCAGGAAGAAACACATAGCAACGGCGAAATTACTCAAACTATTAAAAAATCTAATTATAGTATTAACTGA", "aa": "MYGYKKYINDLTFYHGDGNEWCDQDGCQEETHSNGEITQTIKKSNYSIN", "aa_hexdigest": "9ceab7927a4c10fe9c661039847e1820", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5740.112799999999, "isoelectric_point": 4.734767723083496 }, "id": "DFOKDJOJIG_939", "locus": "GCA013367855_00931" }, { "type": "cds", "sequence": "contig_1", "start": 528006, "stop": 528503, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGCATTTCATCTACAAAATTCAAGTCAAGAAATTATTATAGATTCTGATACTATGGGAAAATTAACGACAGGTATTGATTTAAATATTTTTAAAGGTGGTAAAAGTAATCTTGAAAAACTACAAGAAATTGTTGAACAAATGGATAATGAAACAGCAATTGGCAATTTTATTATTAAATATGGTTATGAAGTCAAACCTATTTCAGATGGAAATCATTTTAATCTAATTTTTAAAATCAAACAATTAAAATATAAGGGAAAAGATATCACTAATTCTTTTGCTTCTAAAATTTTTAATGAAGCAGATACTTATAACTTTGCTAATAATTTTGCCAAAAATCAACAAAGTAAAATTCAAATTAAGAATCTTTATCATATGTTTTATAGTCTTTTTAAAGGCAAGACAATAATGGCTAATGTTCCAGTAAGATTTACTCCAAGCCAAATTTATCAAACTTATGCAGACAAGACAGGATTTGTTAGTAGATTATGA", "aa": "MSAFHLQNSSQEIIIDSDTMGKLTTGIDLNIFKGGKSNLEKLQEIVEQMDNETAIGNFIIKYGYEVKPISDGNHFNLIFKIKQLKYKGKDITNSFASKIFNEADTYNFANNFAKNQQSKIQIKNLYHMFYSLFKGKTIMANVPVRFTPSQIYQTYADKTGFVSRL", "aa_hexdigest": "18837d5f2cab88a1db467aa3e85a243a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18891.375399999983, "isoelectric_point": 9.260125923156735 }, "id": "DFOKDJOJIG_940", "locus": "GCA013367855_00932" }, { "type": "cds", "sequence": "contig_1", "start": 528645, "stop": 528992, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATACTCCACTACAAGTAAATTTTGACACCACATTAGAAGAAAATGAAGTTTTATTTATAAATGGTCAAAAAGTTGATGTTTTAAATAGACATTTTGTTTATCATTTAAAAGATTTAAGACGTTCGGCATCCGATGAAGAAAAAATAGCATTAGAGAAAAATGATTCAAATAAGCCTCAAGAAGAGTTAAATGACGATAATTCAAGAAAGAAAAACGAGTATATAGTTGAAATAAAGAAATTCAAACCTGGCACAAATAATAGCGGAGAGCCAATAGCAACTTATAAAATTAAGTTCGTTATAGTTGAACAACCAATCAACCAATCATTTAAATGGTTTGCTTGA", "aa": "MHTPLQVNFDTTLEENEVLFINGQKVDVLNRHFVYHLKDLRRSASDEEKIALEKNDSNKPQEELNDDNSRKKNEYIVEIKKFKPGTNNSGEPIATYKIKFVIVEQPINQSFKWFA", "aa_hexdigest": "8533c2db2702be0af9ebac919fac8f16", "start_type": "GTG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13495.009399999984, "isoelectric_point": 5.925374031066894 }, "id": "DFOKDJOJIG_941", "locus": "GCA013367855_00933" }, { "type": "cds", "sequence": "contig_1", "start": 529215, "stop": 529448, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAATGATTTTGGTATTTTTGCAGAGGGCGCTGTCCTTGGTAAAGGAGCCTTAAGAAATCTAATCTTAGATAAAAATATTGCCAAAAAATCTAATGTTACATACTATAAATGCAAAATTTATGATAAAGGAAGCAACAAATGGCTTAGTCCTAGTGAAATTAAACCTGAACCATTATCTTCATCAAACGGTAATAGTGAAAATGCTTATATGTCTGAAGAAGGCATCTGA", "aa": "MLNDFGIFAEGAVLGKGALRNLILDKNIAKKSNVTYYKCKIYDKGSNKWLSPSEIKPEPLSSSNGNSENAYMSEEGI", "aa_hexdigest": "b45baf31aa37607b88b8d8fbcdf77cef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8459.5085, "isoelectric_point": 7.798563194274903 }, "id": "DFOKDJOJIG_942", "locus": "GCA013367855_00934" }, { "type": "cds", "sequence": "contig_1", "start": 529875, "stop": 530099, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823", "UniRef:UniRef90_UPI001314F823" ], "nt": "ATGTCAAGCAACACATTAACCAAAAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACCGAGGAAAATAAATTTCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGA", "aa": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEA", "aa_hexdigest": "4cf89e55fbd43bc2c9828114fb6c5739", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 529875, "stop": 530325, "observations": { "insertion": [ 530286 ], "mutation": [ 530097 ], "stop": [ 530097 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314F823", "query_cov": 1.0, "subject_cov": 0.4966442953020134, "identity": 1.0, "score": 145.0, "evalue": 1.93e-43, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEAWHVYNPPIDLSTLDIQNEFILNISKKTFEEKNIPEDVVDERYRKAIEKEVINFITNQLLQYQLKQNLLAKKLKCY" }, "paralog": false, "identity": 0.9798657718120806, "score": 287.0, "evalue": 1.32e-101, "description": "Internal stop codon at 530097. Frameshift due to insertion around 530286. Nonsense mutation around 530097.", "psc": { "uniref90_id": "UniRef90_UPI001314F823", "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314F823", "UniRef:UniRef50_UPI001314F823" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8600.459199999998, "isoelectric_point": 4.854925346374511 }, "id": "DFOKDJOJIG_943", "locus": "GCA013367855_00935" }, { "type": "cds", "sequence": "contig_1", "start": 530133, "stop": 530660, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCAGAAGATGTTATTGATGAAAGATATAGAAAAGCAATAGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCCAATTTAAATGAAGTAATTGCCCATTTAATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTCTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGA", "aa": "MDIQSEFILNISKKTFEEKNIPEDVIDERYRKAIEKEVINFITNQLLQYQLKTKSSGKEIEMLLNKDVEIANLNEVIAHLMRGKALSSFVGLKLVSKNANLHNYKIINFKNLGEGGKFNLRNLSYILMDNKNVIQETKPSQIRKKVIEIVVNAASKLGLEINNDYQIFAINNDAY", "aa_hexdigest": "16cb6b67b60f78684bdb9d0db65ba5e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20119.024600000004, "isoelectric_point": 8.923278617858887 }, "id": "DFOKDJOJIG_944", "locus": "GCA013367855_00936" }, { "type": "cds", "sequence": "contig_1", "start": 530661, "stop": 531014, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358", "UniRef:UniRef50_UPI0027330C60", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef90_UPI001314B421" ], "nt": "TTGGAATTAGTTTCGAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAGGCTCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTCGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATATAAGAAAATTCTTTTATTACCTACAAACAAAACCACCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATAGACCCTGATTGA", "aa": "MELVSKYDDPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTTGFGYGYIINKANKEYNPAIDPD", "aa_hexdigest": "7338646e778a5dfe217af27c3d57dad9", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001314B421", "query_cov": 0.8632478632478633, "subject_cov": 0.9805825242718447, "identity": 0.96, "score": 179.0, "evalue": 1.05e-56, "valid": true, "uniref50_id": "UniRef50_UPI0027330C60" }, "pscc": { "uniref50_id": "UniRef50_UPI0027330C60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027330C60" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 530685, "stop": 531047, "observations": { "mutation": [ 531012 ], "stop": [ 531012 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005C90358", "query_cov": 0.9316239316239316, "subject_cov": 0.4448979591836735, "identity": 0.982, "score": 205.0, "evalue": 6.14e-65, "gene_start": 9, "gene_end": 117, "reference_start": 1, "reference_end": 109, "reference_sequence": "NPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPDWKPNPNPDDEDIVSPEDIPKDPNRIKKVVKTWWFPLVVIVGILTFLVASIFIIKSLLRKYGWSFGKRAKRIRRQNPNKQSYQEKRSNKRQEKLAKKEAKNLKRQTKKQEKANFIQAKKDKKTKNKSKTNNEQNSIN" }, "paralog": false, "identity": 0.975, "score": 239.0, "evalue": 6.85e-81, "description": "Internal stop codon at 531012. Nonsense mutation around 531012.", "psc": { "uniref90_id": "UniRef90_UPI0005C90358", "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef50_UPI0005C90358" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13926.73179999998, "isoelectric_point": 5.6632894515991214 }, "id": "DFOKDJOJIG_945", "locus": "GCA013367855_00937" }, { "type": "cds", "sequence": "contig_1", "start": 531156, "stop": 531380, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823", "UniRef:UniRef90_UPI001314F823" ], "nt": "ATGTCAAGCAACACATTAACCAAAAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACTGAGGAAAATAAATTCCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGA", "aa": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEA", "aa_hexdigest": "4cf89e55fbd43bc2c9828114fb6c5739", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 531156, "stop": 531606, "observations": { "insertion": [ 531567 ], "mutation": [ 531378 ], "stop": [ 531378 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314F823", "query_cov": 1.0, "subject_cov": 0.4966442953020134, "identity": 1.0, "score": 145.0, "evalue": 1.93e-43, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEAWHVYNPPIDLSTLDIQNEFILNISKKTFEEKNIPEDVVDERYRKAIEKEVINFITNQLLQYQLKQNLLAKKLKCY" }, "paralog": false, "identity": 0.9798657718120806, "score": 287.0, "evalue": 1.32e-101, "description": "Internal stop codon at 531378. Frameshift due to insertion around 531567. Nonsense mutation around 531378.", "psc": { "uniref90_id": "UniRef90_UPI001314F823", "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314F823", "UniRef:UniRef50_UPI001314F823" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8600.459199999998, "isoelectric_point": 4.854925346374511 }, "id": "DFOKDJOJIG_946", "locus": "GCA013367855_00938" }, { "type": "cds", "sequence": "contig_1", "start": 531414, "stop": 531941, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCAGAAGATGTTATTGATGAAAGATATAGAAAAGCAATAGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCCAATTTAAATGAAGTAATTGCCCATTTAATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTTTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGA", "aa": "MDIQSEFILNISKKTFEEKNIPEDVIDERYRKAIEKEVINFITNQLLQYQLKTKSSGKEIEMLLNKDVEIANLNEVIAHLMRGKALSSFVGLKLVSKNANLHNYKIINFKNLGEGGKFNLRNLSYILMDNKNVIQETKPSQIRKKVIEIVVNAASKLGLEINNDYQIFAINNDAY", "aa_hexdigest": "16cb6b67b60f78684bdb9d0db65ba5e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20119.024600000004, "isoelectric_point": 8.923278617858887 }, "id": "DFOKDJOJIG_947", "locus": "GCA013367855_00939" }, { "type": "cds", "sequence": "contig_1", "start": 531942, "stop": 532295, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358", "UniRef:UniRef50_UPI0027330C60", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef90_UPI001314B421" ], "nt": "TTGGAATTAGTTTCAAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAAGCCCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTTGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATACAAAAAAATTCTTTTATTACCAACAAACAAAACAATCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATTGACCCTGATTGA", "aa": "MELVSKYDDPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPD", "aa_hexdigest": "c9f7e66eb051396304f5653fddd5b1cc", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001314B421", "query_cov": 0.8632478632478633, "subject_cov": 0.9805825242718447, "identity": 0.95, "score": 178.0, "evalue": 3e-56, "valid": true, "uniref50_id": "UniRef50_UPI0027330C60" }, "pscc": { "uniref50_id": "UniRef50_UPI0027330C60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027330C60" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 531966, "stop": 532577, "observations": { "mutation": [ 532293, 532386, 532389, 532476 ], "stop": [ 532293, 532386, 532389, 532476 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005C90358", "query_cov": 0.9316239316239316, "subject_cov": 0.4448979591836735, "identity": 0.991, "score": 208.0, "evalue": 3.74e-66, "gene_start": 9, "gene_end": 117, "reference_start": 1, "reference_end": 109, "reference_sequence": "NPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPDWKPNPNPDDEDIVSPEDIPKDPNRIKKVVKTWWFPLVVIVGILTFLVASIFIIKSLLRKYGWSFGKRAKRIRRQNPNKQSYQEKRSNKRQEKLAKKEAKNLKRQTKKQEKANFIQAKKDKKTKNKSKTNNEQNSIN" }, "paralog": false, "identity": 0.9605911330049262, "score": 382.0, "evalue": 3.61e-137, "description": "Internal stop codon at 532293, 532386, 532389, 532476. Nonsense mutation around 532293, 532386, 532389, 532476.", "psc": { "uniref90_id": "UniRef90_UPI0005C90358", "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef50_UPI0005C90358" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13938.78549999998, "isoelectric_point": 5.6632894515991214 }, "id": "DFOKDJOJIG_948", "locus": "GCA013367855_00940" }, { "type": "cds", "sequence": "contig_1", "start": 533568, "stop": 534188, "strand": "+", "frame": 3, "gene": null, "product": "Mbov_0400 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0", "UniRef:UniRef90_UPI00069702F0" ], "nt": "ATGAATCATCAAAGTGTAGTAGTCACCAAAGATATTTTTATAATTGATAAAGATGTTTTAGAAGCCAATGTAGATCAAAACATCTATCAAGAAACCTTGACATTAAATAAAAACGATAAATCTTTAATAATTAGCGATTTAGAACAAAGGATTAATTCAATTCCACCTGATTTAAATATTTTAAAAATATCTAATAACTTAAAAAGCAATTTAGTTTTATACACTAATGATAGCCTAGTTAATTCTCAGTCTAATTCGATTTTGAAAGGCAAAAATTACAAAATTAAAAAGGCTACTAAAAATTATTACAAACATAATTTTAACAAATCTCAATTTCTTAAAGATACAAACACGTCAAATATAGAACATACTAAGCAAGCCCTAAAAAACATAAAGTTATGCATAAATAAAGCTAAAAAAATTGATTATACTAATTTTGAATATATTTTAGATGTTCAAACAAACACATATCTATTTCATCTAATTCCCTCTTCTCTATCCGATTTTGATAAAACACCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACCAATCTCAGAAAGAGTCCAAAGCTAGCCAATTCCGCTCAAAACCTCATAATCTAAAACATTAG", "aa": "MNHQSVVVTKDIFIIDKDVLEANVDQNIYQETLTLNKNDKSLIISDLEQRINSIPPDLNILKISNNLKSNLVLYTNDSLVNSQSNSILKGKNYKIKKATKNYYKHNFNKSQFLKDTNTSNIEHTKQALKNIKLCINKAKKIDYTNFEYILDVQTNTYLFHLIPSSLSDFDKTPTDLQDSKVINEYNQSQKESKASQFRSKPHNLKH", "aa_hexdigest": "3769969c99795e10e2a3819124aca02d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 533346, "stop": 534188, "observations": { "mutation": [ 533361, 533565 ], "stop": [ 533361, 533565 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00069702F0", "query_cov": 1.0, "subject_cov": 0.7357142857142858, "identity": 1.0, "score": 389.0, "evalue": 1.44e-135, "gene_start": 1, "gene_end": 206, "reference_start": 75, "reference_end": 280, "reference_sequence": "MSLELWKPLKITNPLLEDIFHLNIDTKFKIHPLIIFENKENKTYYCIRLQTSTKTTKKHNLQIDNSSYQTNEYWMNHQSVVVTKDIFIIDKDVLEANVDQNIYQETLTLNKNDKSLIISDLEQRINSIPPDLNILKISNNLKSNLVLYTNDSLVNSQSNSILKGKNYKIKKATKNYYKHNFNKSQFLKDTNTSNIEHTKQALKNIKLCINKAKKIDYTNFEYILDVQTNTYLFHLIPSSLSDFDKTPTDLQDSKVINEYNQSQKESKASQFRSKPHNLKH" }, "paralog": false, "identity": 0.9928571428571429, "score": 553.0, "evalue": 1.03e-202, "description": "Internal stop codon at 533361, 533565. Nonsense mutation around 533361, 533565.", "psc": { "uniref90_id": "UniRef90_UPI00069702F0", "product": "Mbov_0400 family ICE element protein", "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00069702F0", "UniRef:UniRef50_A0A454C8W0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0" ] } }, "id": "DFOKDJOJIG_949", "locus": "GCA013367855_00941" }, { "type": "cds", "sequence": "contig_1", "start": 534225, "stop": 534365, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATAGATTTTTATATTAATGGTGTAAGCGAAAAAAGTATCTTATCCTCAGGCCAATTAATAGTAGAAAAATTGAACATCGAAGAAGAAATATTTCGTACTAAAACAAGGTTTGAAAATAATCAATATAGTAATTGA", "aa": "MEIDFYINGVSEKSILSSGQLIVEKLNIEEEIFRTKTRFENNQYSN", "aa_hexdigest": "da1f63fc5604fbd82d1459e9fb83b08a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5428.0015, "isoelectric_point": 4.644962120056152 }, "id": "DFOKDJOJIG_950", "locus": "GCA013367855_00942" }, { "type": "cds", "sequence": "contig_1", "start": 534741, "stop": 535103, "strand": "+", "frame": 3, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005A7E644", "UniRef:UniRef50_UPI0013142BC0", "UniRef:UniRef90_UPI0005A7E644", "UniRef:UniRef90_UPI0013142BC0" ], "nt": "ATGGTTAGAATGGTTGGATTTCACTCTGGATATTTAAACCAAAATAACCATATGTTATTAAATAAAACCATTATTTTTCAGTTTAACAAAAGTCAAAAAATTTATCAAAATATAGATATCTTAGCCACTAAAACCAGCGAAATTTTAAATAACTTTTACCATGCAAAAACTATTAATTTATCAGGAGATGGAGCAAGAATAATAACTAAATTTAGCGAACAAATTAATGCTATTAGGTATTATGATAAGTTCCATTTTCACAAAATATTATTTGATGTTTTTGGGTATTCAAGAAAGAAAAATAAGGATAATAAGAGAATATTTGATAATACATTTGGGAATCAATTTTCTCAATTTTTGTGA", "aa": "MVRMVGFHSGYLNQNNHMLLNKTIIFQFNKSQKIYQNIDILATKTSEILNNFYHAKTINLSGDGARIITKFSEQINAIRYYDKFHFHKILFDVFGYSRKKNKDNKRIFDNTFGNQFSQFL", "aa_hexdigest": "cf62241933c3fbb644ce3d03a7aa80fc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0005A7E644", "query_cov": 0.975, "subject_cov": 1.0, "identity": 0.94, "score": 226.0, "evalue": 6.52e-75, "valid": true, "uniref50_id": "UniRef50_UPI0005A7E644" }, "pscc": { "uniref50_id": "UniRef50_UPI0005A7E644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005A7E644" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 534651, "stop": 535406, "observations": { "mutation": [ 535101, 535224 ], "stop": [ 535101, 535224 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013142BC0", "query_cov": 1.0, "subject_cov": 0.47244094488188976, "identity": 0.992, "score": 241.0, "evalue": 4.85e-79, "gene_start": 1, "gene_end": 120, "reference_start": 31, "reference_end": 150, "reference_sequence": "MKNTKFLPSKNDVIQLENDDAYINLNKGKFMVRMVGFHSGYLNQNNHMLLNKTIILQFNKSQKIYQNIDILATKTSEILNNFYHAKTINLSGDGARIITKFSEQINAIRYYDKFHFHKILFDVFGYSRKKNKDNKRIFDNTFGNQFSQFLWFLNNKKYEDFENKLHENLIYLRENKLSAIKIVEIKSLIKLWKNNKNYLINSYENANYYGGNAETFIGHYLKKYTKTAFSRHNIDYLKLKILLNIPQNLNVVFL" }, "paralog": false, "identity": 0.9840637450199203, "score": 499.0, "evalue": 4.17e-183, "description": "Internal stop codon at 535101, 535224. Nonsense mutation around 535101, 535224.", "psc": { "uniref90_id": "UniRef90_UPI0013142BC0", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_UPI0013142BC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013142BC0", "UniRef:UniRef50_UPI0013142BC0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013142BC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013142BC0" ], "product": "Mbov_0401 family ICE element transposase-like protein" } }, "id": "DFOKDJOJIG_951", "locus": "GCA013367855_00943" }, { "type": "cds", "sequence": "contig_1", "start": 535775, "stop": 535948, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATAGAAAAATCTCTTTATGATTTTGCGACCCCTGATTTTCAAACACTTCCAATCTTTTTTGATAAAAATAACGACATTATTAAATTATTACCAGCAGATTCACAAACAAAAATTGATGATTATCTAGACAAAATTAAAAAAATTACAGAAAAGAATAGTCAAGCCAAATGA", "aa": "MIEKSLYDFATPDFQTLPIFFDKNNDIIKLLPADSQTKIDDYLDKIKKITEKNSQAK", "aa_hexdigest": "a5d15f173ec848f01a7d8f6217bd6452", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6651.544200000002, "isoelectric_point": 5.116555213928222 }, "id": "DFOKDJOJIG_952", "locus": "GCA013367855_00944" }, { "type": "cds", "sequence": "contig_1", "start": 536057, "stop": 536263, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAAATAATTTAAAAGAAATTAAAAAACAAAAGAAAAATGATTATGACACGATTGTTACCTCTTATTTAAAAGAAAAAACAGAAGAAACAATAACAAAAAATGAATCAATAATTGCTAAAAAATCTAATATTGAAAGTCAAAATATTATTGAAACGTTTGTTTTATTGCAAAAATTAGAACTTGAATTTGCTTACATTAATTGA", "aa": "MTNNLKEIKKQKKNDYDTIVTSYLKEKTEETITKNESIIAKKSNIESQNIIETFVLLQKLELEFAYIN", "aa_hexdigest": "575c0829f55184b1988357337f0dcdb7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8012.098699999996, "isoelectric_point": 6.252255058288575 }, "id": "DFOKDJOJIG_953", "locus": "GCA013367855_00945" }, { "type": "cds", "sequence": "contig_1", "start": 536681, "stop": 536953, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATGATATGTTTGAAAAAACGAGCGAGGCTAGTGTTTATAAAATAAAAACATTCTTACCTGATAAAACTGATCGTGATGAAAGAAAATTATATTTTGATAAAAGCCTTCCTTTATTTAAATTATTTAGCAAAGTAAGTGTAAATTTTAATACATTTTTAGGGGGAACAAAAACTAAAGGAATTTATGAACAAGATGGGAAACTAAGATTTGAAGGATTTATTCAATTTTATGACCCAGAAATCCCTTGTTATGGATATTTTGGATGA", "aa": "MINDMFEKTSEASVYKIKTFLPDKTDRDERKLYFDKSLPLFKLFSKVSVNFNTFLGGTKTKGIYEQDGKLRFEGFIQFYDPEIPCYGYFG", "aa_hexdigest": "e331f47f48ce641afa26f606dfa366e6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10595.007499999996, "isoelectric_point": 7.760875511169434 }, "id": "DFOKDJOJIG_954", "locus": "GCA013367855_00946" }, { "type": "cds", "sequence": "contig_1", "start": 537056, "stop": 537502, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCACCAAGCGATGAATTTTTAAAAATTGAAGAAGAATTTCAAGACCAAAAGAAGCAAATTGAATCTAAACTAGATGAAATTTGCTCTCTAAAATTTAATAAGACATACAGCGAACAATTAATTAAATCAAATGAATCTATAAAGAGCGCAATTAATAAAAAAGTTAATAACGAAGTTTATGGTGAATACCTGGGAACTGAAGAAAGATTATATTATAGAAAAGCCTATAATGAAAGAAATAAAGAAGATTGTAAGAAATATATAAATCAACTTAAAAATATTTATAAGATAGTTGATATGTATGCTAATTATTTAAAAGAAGTATTCAAATATATTGAATCAGAAGAAGCAAAATTAAGTGATGAAATAAAGAAAAAAATAATGGACGAAGATGTAATAGAAATTACCAAAACTAGTAGAAGCAAAATCGAATATTTAAATTGA", "aa": "MPPSDEFLKIEEEFQDQKKQIESKLDEICSLKFNKTYSEQLIKSNESIKSAINKKVNNEVYGEYLGTEERLYYRKAYNERNKEDCKKYINQLKNIYKIVDMYANYLKEVFKYIESEEAKLSDEIKKKIMDEDVIEITKTSRSKIEYLN", "aa_hexdigest": "a454da64d70a8dd57c002e889b15a3a3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17802.028000000006, "isoelectric_point": 5.511870384216308 }, "id": "DFOKDJOJIG_955", "locus": "GCA013367855_00947" }, { "type": "cds", "sequence": "contig_1", "start": 537809, "stop": 538351, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAGTCTAAGCAAGAATTACAAAATCTTATTGATTTTAGCGCTGACCAAGGAATTGAAAATTCAAAAGCAAAAGAAATTTTAGATTCAACAAACATTGAAGAAAATTCTTCAATTAGTGAGGTTAAAGAAAAAACAAACAAAATTATTAAAGCTAAACAAGATCTTCAAAATTTAATAGAAGATACCAAAAATAAAGCAAAAAATGAGTTTGATATTAAAAAACAAGAATTGAGAGTACTTCTAGATTCAATAGACAATAAGATTAATAAACAAGACGAAGAAGAAAAATATAAGAATATTGAAGTTAAAGATAATGATTTAGTTAAAGACATTAAAAATAAGACTAATGAAATTTCAAAAGCAATTAAATCATTGAATAAAAAAATTAATGAACAAGAATTTGAACAAGCAAAACAAGATTTTGAAAAAATAAAAAGAGAACTACAAGAATTAATTGCAACCCCTGATGCAAAAGAAGTAGGAAACATTGATGATATAAACAAAACCTTGAATGAAGCAAACATTGCCCAAGATTGA", "aa": "MKKSKQELQNLIDFSADQGIENSKAKEILDSTNIEENSSISEVKEKTNKIIKAKQDLQNLIEDTKNKAKNEFDIKKQELRVLLDSIDNKINKQDEEEKYKNIEVKDNDLVKDIKNKTNEISKAIKSLNKKINEQEFEQAKQDFEKIKRELQELIATPDAKEVGNIDDINKTLNEANIAQD", "aa_hexdigest": "5f878d77fdb8a12ed10e83e38dc41441", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20803.0815, "isoelectric_point": 5.082963371276854 }, "id": "DFOKDJOJIG_956", "locus": "GCA013367855_00948" }, { "type": "cds", "sequence": "contig_1", "start": 538523, "stop": 538708, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAAATTTATCATTACAAATTTTAGCGTTGTGTGTAGCAGCGGGCTCTGTGCCTGTTTTAGCATCTAAATGTACCGTAACTGTAAAAGTTAAAGAAAAGTTTAATGCTCTAAAATCACAACTTCAAAATTTAATTTCAAAACTAAGTGATGAAGAACAACAAGAACTTAATGAATGA", "aa": "MKKKNLSLQILALCVAAGSVPVLASKCTVTVKVKEKFNALKSQLQNLISKLSDEEQQELNE", "aa_hexdigest": "4a25dd07e169d330eef575d4d9523fbb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6700.840199999999, "isoelectric_point": 9.064206504821776 }, "id": "DFOKDJOJIG_957", "locus": "GCA013367855_00949" }, { "type": "cds", "sequence": "contig_1", "start": 538922, "stop": 539470, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGATCAAAAAGATTTTAATATTATAGATAGTGAAATAGCAGCATTTGGCAATGTAAAAAAGATCTTTGATGAAAATATAAACAATATAAATGATATTTGTAAAGAAATAATTAAACAGGAATTTTTAGAAAAAAATTCAAATAACAAAAGAATTGAAGAAGCTAAAAAACAATACGAAATAGTAAGAGATTCTAATGAAAGAATTATTGCTGAAAAAAAGCTAAAAAAAATATCAGGAAGGAAATTTGCAAATTGCATAATTTTTATACTTTTCTTTATTCTAATTGGTTTTTGTTTTTTTTCATTTTTTGCTAACAATAAGAAAATAATTAAATCATTTGAAGAGTTTAGAAATAATGAACAATTAAAAATTGATGAAGCTTTAGGCAAGGCAAGACAGGCATACGTTGAAACCTTTTCATTACAAACCCTTGAAGATATAAAAAATAAAATTTTAAGCAAAATGGGGATAAAAAAAATATCACAATACGATGCTAAAGTTGGTGATTTTTCGCGTAGTTTTATTTATGATAAAAATTTTTGA", "aa": "MEDQKDFNIIDSEIAAFGNVKKIFDENINNINDICKEIIKQEFLEKNSNNKRIEEAKKQYEIVRDSNERIIAEKKLKKISGRKFANCIIFILFFILIGFCFFSFFANNKKIIKSFEEFRNNEQLKIDEALGKARQAYVETFSLQTLEDIKNKILSKMGIKKISQYDAKVGDFSRSFIYDKNF", "aa_hexdigest": "f951a34a3d21b3d8f542a5c3ea7f1081", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21445.501199999995, "isoelectric_point": 8.96937351226807 }, "id": "DFOKDJOJIG_958", "locus": "GCA013367855_00950" }, { "type": "cds", "sequence": "contig_1", "start": 539894, "stop": 540802, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA032", "UniRef:UniRef90_UPI00215DA032" ], "nt": "ATGAATCAAGAAAACATGCCGGTATTTTCAATGATAAAAAATGGAAATTGGTTGGTTTCTAAAAATACGATTCAATCAAGTTCCTTATTATTTGAACAAGTTCAAGATTGGATGTTAAGTGTTTTGGATTTAACTGAAGAACTAACAGTTGACTTTGTCACGGAATTTTTAAGTTCAAAAATTATTGAATGTTTATTGCCAATATTTAATTTTATTTCCTTAGTATATACAAATAAGTATATTGCAACTGAAAATTTAGAAAAAACAGAAAATAGATATGAACAAGACCTTGTTGTTAAACAAAATAACAATGTCCAAAATTACCCTCTAAACATTTACTCATATTATAGAGACATGACATTATATAGTGGGTTTTATAAATTTAACAAGCATGCAGATATAACACCAATTTGCGAATTTAAAAATCTTCATAATGACCCAGAATCTTTGATAATATCAGCTACATTTGATTTGATTTCATATACAGCCAGAGACGCAATTGCAATTGAATATACACATGGTTACGCCGTTGAAGTACCATATGTGGCATATGATGAATTTAGAGAACATAAAGCAATAATTGTTGCTCCTTATAATATAAAAACAGAAAATGCCAAAATATTTGCAAATAGTCTTATTGCTAATAATATTTATGTAAGCGATGATCGCATAGATGAAGAATATAGCAAAATTATCCAAAAAAATAAAATTTCATTTAATTTAGAATCATTAAAGGACTTTGATGAATTTAAGAAAAATATTAAGCTTATAGATGAATTATTTAGTCAAATTCCTGAAATAAAAAAGATTCCAATACTACTTGATAATAGAGGAATGGCTTTCTTTATAAATGAGCCAAAATTATTTGACATCAATTTATTAATTAAAAAGCTTAATAATTTTTATTAA", "aa": "MNQENMPVFSMIKNGNWLVSKNTIQSSSLLFEQVQDWMLSVLDLTEELTVDFVTEFLSSKIIECLLPIFNFISLVYTNKYIATENLEKTENRYEQDLVVKQNNNVQNYPLNIYSYYRDMTLYSGFYKFNKHADITPICEFKNLHNDPESLIISATFDLISYTARDAIAIEYTHGYAVEVPYVAYDEFREHKAIIVAPYNIKTENAKIFANSLIANNIYVSDDRIDEEYSKIIQKNKISFNLESLKDFDEFKKNIKLIDELFSQIPEIKKIPILLDNRGMAFFINEPKLFDINLLIKKLNNFY", "aa_hexdigest": "0f19d1dd9830ecb72b50886c4e170652", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 539594, "stop": 540802, "observations": { "mutation": [ 539609, 539621 ], "stop": [ 539609, 539621 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215DA032", "query_cov": 1.0, "subject_cov": 0.6425531914893617, "identity": 0.97, "score": 574.0, "evalue": 7.21e-204, "gene_start": 1, "gene_end": 302, "reference_start": 169, "reference_end": 470, "reference_sequence": "MGIKKISQYDAEVGDFSRSFIYDKNFWNFNSISQYKLRNSYIYDIFYTCKWMKNVVTKASITVTEEDANGNTYWETYWAYHTEPTPVVEYFRSLTIPSNYKQKLYFEIIENNRSEKEVKRLLKNEGFHLENKEVYQNFDFAFSDDQKIDFLTYFDVRTQQNLINYAQYMNQENMPVFSMIKNGNWLVSKNTIQSSSLLFEQVQDWMLSVLDLTEELTVDFVTEFLSSKIVECLLPIFNFISLVYTNKYIATENLEKTGNRYEQDLVVKQNNNIQNCPLDIHSYYRDMTLYSGFYKFNKHADITPICEFKNLYNDPESLIISATFDLISYTARDAIAIEYTHGYAVEVPYVAYDEFREHKAIIVVPYNIKTENAKIFANSLIANNIYVSDDRIDEEYSKIIQKNKISFNLESLKDFDEFKKNIKLIDELFSQIPEIKKIPILLDNRGMAFFINEPKLFDINLLVKKLNNFY" }, "paralog": false, "identity": 0.972636815920398, "score": 788.0, "evalue": 1.37e-290, "description": "Internal stop codon at 539609, 539621. Nonsense mutation around 539609, 539621.", "psc": { "uniref90_id": "UniRef90_UPI00215DA032", "uniref50_id": "UniRef50_UPI00215DA032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215DA032", "UniRef:UniRef50_UPI00215DA032" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA032" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35439.0854, "isoelectric_point": 4.91523151397705 }, "id": "DFOKDJOJIG_959", "locus": "GCA013367855_00951" }, { "type": "cds", "sequence": "contig_1", "start": 540890, "stop": 541447, "strand": "+", "frame": 2, "gene": "lemA", "product": "Protein LemA", "db_xrefs": [ "COG:COG1704", "COG:M", "GO:0005886", "KEGG:K03744", "SO:0001217", "UniRef:UniRef50_P43054", "UniRef:UniRef90_P43054" ], "nt": "ATGTTATTTGATTCAAGAACCCCACAATCTAGTGAAGGATTTAAACCAAACGTAGATAATAGCATTAAAAAACCAATTCCAACAGGTGTTGAAAAATTCTTTTTTATCTTATTTTTTATACTTACAATAGGAATTTTCTATTTTGTATATGTAGGAAGAAAAAATGAATTAATGCGTGATCAAAACGAAATTCAAAATGCAAGTTCTCTAATACAAGCAGCAGAAAAAAGGAGACGTGCAGTTCTAATTAAAATGATGGATAGTTTAATTGGCTATAAAAATTTTGAAAATGAAACATTAACTAAAATAACTCAATATAGATCAAAATTATCAAATATCGATGTAGACAAAACTTCTCCCGTTGAATTGAAATCACAAATTGACAGCATCAGAGGTGCTTTGAACTTTCAATTTGAACAATACCCAGATCTTAAAGCAAGCAAATTGTATTTACAATTTTCAACCGAAATTTCTATGCAAGAAGATGAAATTTATGCAACAATAAGAAACTATAATATGATTGCAACATCATTTAATTCAAAAATATATACATTTTGA", "aa": "MLFDSRTPQSSEGFKPNVDNSIKKPIPTGVEKFFFILFFILTIGIFYFVYVGRKNELMRDQNEIQNASSLIQAAEKRRRAVLIKMMDSLIGYKNFENETLTKITQYRSKLSNIDVDKTSPVELKSQIDSIRGALNFQFEQYPDLKASKLYLQFSTEISMQEDEIYATIRNYNMIATSFNSKIYTF", "aa_hexdigest": "181baf512e89f9176479d877cbeb4d28", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_P43054", "query_cov": 1.0, "subject_cov": 0.8447488584474886, "identity": 0.995, "score": 357.0, "evalue": 3.14e-124, "valid": true, "gene": "lemA", "product": "Protein LemA", "uniref50_id": "UniRef50_P43054", "cog_id": "COG1704", "cog_category": "M", "kegg_orthology_id": "K03744", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P43054", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43054" ], "product": "Protein LemA" }, "genes": [ "lemA" ], "id": "DFOKDJOJIG_960", "locus": "GCA013367855_00952" }, { "type": "cds", "sequence": "contig_1", "start": 541635, "stop": 541769, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAAATAGAAGAAATACATAAATATAATGCCGATAATATTCAAATATTAGAAGGTTTAGAAGCCGTAAGAAAAAGACCCGGCATGTACATAGGCTCAATAGGGTTCAAGGGTTTGCACCACTTGCTATGA", "aa": "MDKIEEIHKYNADNIQILEGLEAVRKRPGMYIGSIGFKGLHHLL", "aa_hexdigest": "8d4f09ee56f4b74af8dfcccb6640f0f0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5048.840599999998, "isoelectric_point": 6.81854190826416 }, "id": "DFOKDJOJIG_961", "locus": "GCA013367855_00953" }, { "type": "cds", "sequence": "contig_1", "start": 541776, "stop": 542045, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "GTGGATAACTCAGTCGATGAAGCAATGGCGGGTTTTGCTACTGAAATTAAAATTAAATTGTATCCAAATAATGTAATAGAAGTTGAAGACAATGGTCGTGGAATGCCCACTGGAATTTACTCTGGAACTAAAAAGTCAGCTGTCGAAACAATTTTAACCGTGCTACATGCTGGTGGTAAATTTGATGGATCAAATTATAAAGTTTCCGGAGGATTACATGGTGTTGGTGCATCAGTTGTTAATGCATTAAGTAGTGAATTTGAAGTATGA", "aa": "MDNSVDEAMAGFATEIKIKLYPNNVIEVEDNGRGMPTGIYSGTKKSAVETILTVLHAGGKFDGSNYKVSGGLHGVGASVVNALSSEFEV", "aa_hexdigest": "3e9cdab9b523cd72d274123f636de4aa", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0034D3B81C", "query_cov": 0.9887640449438202, "subject_cov": 0.9166666666666666, "identity": 0.591, "score": 109.0, "evalue": 1.5e-29, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_Q706I4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 88, "aa_cov": 0.9550561797752809, "hmm_cov": 0.8181818181818182, "evalue": 5.068499650842438e-12, "score": 41.91386795043945, "start": 1, "stop": 85 } ], "seq_stats": { "molecular_weight": 9292.3208, "isoelectric_point": 4.9471750259399405 }, "id": "DFOKDJOJIG_962", "locus": "GCA013367855_00954" }, { "type": "cds", "sequence": "contig_1", "start": 542109, "stop": 543419, "strand": "+", "frame": 3, "gene": "gyrB", "product": "DNA gyrase subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P43053", "UniRef:UniRef90_P43053" ], "nt": "TTGGAAGTAATTGGAAATGTTTCTGAAGTTGAAACAGGAACAACAATTAAATTTCACCCTGACTATACCATAATGGAAAAAGAAAATTTTGACTTTGATACAATTATTGACCACTCCAAACAAATTGCTTATTTAAACAAAGGTTTGAAAATAACCGTTGAAAATGTTGAAAAAAATATCATCAAAGTTTTTTGTTTTGAAGGTGGACTAATAGACTATGTCAAAGAACTAAACAAAGGTAAAAAATTAATAGTTCCCGAAGTTATTTATGCAGAGGGAGTTTTTAACGATAAAAACTTTACAAATGGACAAGATGTAATAGTAGAAGTTGCAATGCAATATAATGAAGCCTACACAAATAGTATTGTTTCTTATGCAAACAATATTCAAACAATTGATGGTGGAACACATGAACAAGGTTTCTATGATGCATTAGTAAGAATTTACAATAATTACGCCGAAACAAATAAACTATTTAAAACTAGCTCAGAAAAAATAACAAGAGAAGATGTTAAGGAAGGTTTAGTAGCAATCATTTCTATTAAACACACAGACCCAATTTTTGAAGGTCAAACTAAAGGAAAATTAGAAAATAAAGATGCAAGAATTGCCACAAATAAAATTCTTTCAGACTCACTAGAACGTTATTTGAATGAAAACCCAGAAATTGCAAGAGCAATAATCGAAAAGTGTCTTCTTTCGCAACACACAAGGCTTCTTGAAATAAAGGCTCGCGAAGCTTCTAGAAAAGGTAATGGTTTAGATTTAGGTAATCTTCCAGGAAAATTAGCAGACTGTTCATCGAAAAATGCAGAAATTAGAGAATTATTTATTGTCGAAGGTAATTCAGCCGGAGGTTCTGCTAAAATGGGAAGAGATCGTTCTATTCAAGCTATTTTGCCTCTACGTGGTAAGGTTATAAATGCAGAAAAAAATTCGTTTGCTTCTGTCTTGTCAAATAAAGAAATTGCAACAATGATTCACGCCTTAGGCACAGGAATAAATACAGAATTTGATATTAACAAATTAAAATATCACAAAATTATCATTATGACAGATGCCGACGTTGATGGAGCACACATTACTACTTTATTATTGACATTCTTTTACCGTTATATGAAACCTTTAATTGAATATGGATTTGTTTATTTGGCACAACCTCCACTATATAAAATAACTAGCGGAAAAAATGTTGAATATGCATATAATGATTTACAAAAAGAACAAATCATGGCAAAATTAGAAGATAAAAGAAATGTTGCTATTCAACGTTACAAAGGTCTTGGTGAAATGGACCCAGAACAACTATGA", "aa": "MEVIGNVSEVETGTTIKFHPDYTIMEKENFDFDTIIDHSKQIAYLNKGLKITVENVEKNIIKVFCFEGGLIDYVKELNKGKKLIVPEVIYAEGVFNDKNFTNGQDVIVEVAMQYNEAYTNSIVSYANNIQTIDGGTHEQGFYDALVRIYNNYAETNKLFKTSSEKITREDVKEGLVAIISIKHTDPIFEGQTKGKLENKDARIATNKILSDSLERYLNENPEIARAIIEKCLLSQHTRLLEIKAREASRKGNGLDLGNLPGKLADCSSKNAEIRELFIVEGNSAGGSAKMGRDRSIQAILPLRGKVINAEKNSFASVLSNKEIATMIHALGTGINTEFDINKLKYHKIIIMTDADVDGAHITTLLLTFFYRYMKPLIEYGFVYLAQPPLYKITSGKNVEYAYNDLQKEQIMAKLEDKRNVAIQRYKGLGEMDPEQL", "aa_hexdigest": "bc75bad8e7b5d15584c7dc0d235ae5dd", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI000D1D9E0B", "query_cov": 0.8555045871559633, "subject_cov": 0.8923444976076556, "identity": 0.517, "score": 389.0, "evalue": 1.98e-129, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_F5VUB3" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 541809, "stop": 543581, "observations": { "mutation": [ 542043, 543417 ], "stop": [ 542043, 543417 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43053", "query_cov": 1.0, "subject_cov": 0.6728395061728395, "identity": 0.998, "score": 856.0, "evalue": 4.02e-310, "gene_start": 1, "gene_end": 436, "reference_start": 159, "reference_end": 594, "reference_sequence": "MDKIEEIHKYNADNIQILEGLEAVRKRPGMYIGSIGFKGLHHLLWEIVDNSVDEAMAGFATEIKIKLYPNNVIEVEDNGRGMPTGIHSGTKKSAVETILTVLHAGGKFDGSNYKVSGGLHGVGASVVNALSSEFEVWVKRDGKLHYQQFRNGGIPVKPLEVIGNVSEVETGTTIKFHPDYTIMEKENFDFDTIIDHSKQIAYLNKGLKITVENVEKNIIKVFCFEGGLIDYVKELNKGKKLIVPEVIYAEGVFNDKNFTNGQDVIVEVAMQYNEAYTNSIVSYANNIQTIDGGTHEQGFYDALVRIYNNYAETNKLFKTSSEKITREDVKEGLVAIISIKHTDPIFEGQTKGKLENKDARIATNKILSDSLERYLNENPEIARAIIEKCLLSQHTRLLEIKAREASRKGNGLDLGNLPGKLADCSSKNAEIRELFIVEGNSAGGSAKMGRDRSIQAILPLRGKVINAEKNSFASVLSNKEIATMIHALGTGINTEFDINKLKYHKIIIMTDADVDGAHITTLLLTFFYRYMKPLIEYGFVYLAQPPLYKITSGKNVEYAYNDLQKEQIMAKLEDKRNVAIQRYKGLGEMDPEQLWETTMDPETRKMLQVQIDDAAICDTVFATLMGEEIEPRHDFIQENAKYANNIDI" }, "paralog": false, "identity": 0.9949152542372881, "score": 1158.0, "evalue": 0.0, "description": "Internal stop codon at 542043, 543417. Nonsense mutation around 542043, 543417.", "psc": { "uniref90_id": "UniRef90_P43053", "gene": "gyrB", "product": "DNA gyrase subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P43053", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0005694", "GO:0005737", "GO:0005524", "GO:0003677", "GO:0034335", "GO:0046872", "GO:0006265", "GO:0006261" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43053", "EC:5.6.2.2", "UniRef:UniRef50_P43053", "COG:COG0187", "COG:L", "GO:0005694", "GO:0005737", "GO:0005524", "GO:0003677", "GO:0034335", "GO:0046872", "GO:0006265", "GO:0006261" ] }, "pscc": { "uniref50_id": "UniRef50_P43053", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43053" ], "product": "DNA gyrase subunit B" } }, "id": "DFOKDJOJIG_963", "locus": "GCA013367855_00955" }, { "type": "cds", "sequence": "contig_1", "start": 543429, "stop": 543581, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCCAGAAACTAGAAAAATGCTCCAAGTTCAAATAGATGATGCAGCAATTTGTGATACAGTATTTGCTACATTAATGGGAGAAGAAATAGAACCTCGTCATGATTTTATTCAAGAAAACGCAAAATACGCAAATAATATAGATATCTAA", "aa": "MDPETRKMLQVQIDDAAICDTVFATLMGEEIEPRHDFIQENAKYANNIDI", "aa_hexdigest": "21e2080d0bfa91ac8070ba9604ef6d73", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5754.417799999999, "isoelectric_point": 4.176893043518065 }, "id": "DFOKDJOJIG_964", "locus": "GCA013367855_00956" }, { "type": "cds", "sequence": "contig_1", "start": 543583, "stop": 543768, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAGCCTCTAACTAATCAAGGTTTCACAAACAAAGTATTTTACGATGATGAAACTAATAGATTTATAAAAATAAAATCATATGATGGATTTAACCATAAAAGCGATGCGTTCTTATTAAATAATTTAGATTTTTGTCCAAAGATATTTGTTGATAATAAAAAAGAACTTCAAACCGAATGA", "aa": "MLKPLTNQGFTNKVFYDDETNRFIKIKSYDGFNHKSDAFLLNNLDFCPKIFVDNKKELQTE", "aa_hexdigest": "beb625d91cc544b720c1c5a13551fe00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7235.125100000001, "isoelectric_point": 6.551284980773925 }, "id": "DFOKDJOJIG_965", "locus": "GCA013367855_00957" }, { "type": "cds", "sequence": "contig_1", "start": 543799, "stop": 544023, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGATGACATTTTAAAAACTATTGGAAAAAATTTAATCACTTTGCATAATTCAAAATTGAAATTTTATAAAGAAAATCAAATTGCTAGAAGATTTAATATTTATAGAAAAAAAATTTCTAGTTTAAATAGAAAAATTCCAATCCTAGATAAATACTATAAAAAAATTAATTTGTTTTTAAGAAATATTGACAATTCCGCCCCTGTCCATAATGACCTTTGA", "aa": "MTDDILKTIGKNLITLHNSKLKFYKENQIARRFNIYRKKISSLNRKIPILDKYYKKINLFLRNIDNSAPVHNDL", "aa_hexdigest": "19fc795c88b81a826147d5e7c4888cec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8862.315299999995, "isoelectric_point": 10.167453956604003 }, "id": "DFOKDJOJIG_966", "locus": "GCA013367855_00958" }, { "type": "cds", "sequence": "contig_1", "start": 544084, "stop": 544239, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGATGTTCATTTTGACCTTGCCTATTTTATTGAATCAAGCAATCTAAATGAAAAACAAGAAAAAGTGTTTTTAGATGCTTATGGTGATGATTTTGAACCCAAATATTTATTTATTCACAAAATTTTGGTAAATGCCTTAATTGTGCTATGA", "aa": "MGDVHFDLAYFIESSNLNEKQEKVFLDAYGDDFEPKYLFIHKILVNALIVL", "aa_hexdigest": "7b8a2a13d8a2be708d1059d9310e35ac", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5963.7613999999985, "isoelectric_point": 4.547426414489746 }, "id": "DFOKDJOJIG_967", "locus": "GCA013367855_00959" }, { "type": "cds", "sequence": "contig_1", "start": 544334, "stop": 544537, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAAGAGATTGTTGTTGTGGTTGCAAGTCAGCTGAGTTCTGCACATGCAAAAATAAAAAATCATGTATTCCTAATAATTTATATGAAAAAATTAGGAATGTTTTTGGTTATGAAGTTTTTGAAAAACTTAATAATTTAAGACCTTATTTTGACGATTTACATAGTAGCACATATATTGGAAAACTAGATGATGTTTGA", "aa": "MLKRDCCCGCKSAEFCTCKNKKSCIPNNLYEKIRNVFGYEVFEKLNNLRPYFDDLHSSTYIGKLDDV", "aa_hexdigest": "41eadcc39d14190c748f3075323eae69", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7822.973599999999, "isoelectric_point": 8.167257881164552 }, "id": "DFOKDJOJIG_968", "locus": "GCA013367855_00960" }, { "type": "cds", "sequence": "contig_1", "start": 544964, "stop": 545272, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTATTCCAAAAGAAGATATAATTGATTTCTTTAAATTAGACCCTTCTGTATTTGATGATTTTATTTTTTTAATGAGAGTTTACAATGAGGCAATGTATTTAAATGATTATTCAAAAAATAATTCCAAATCGCTAAGCCCTTTTGATTCAAAGAGTTTATATAGCAACAAGGATTTCTTAGAATTAAATAGATTCATTGTTCAAAAAAATCATAATAACTTTGACAATAAATTAAATATTTCTAAAATTGAAAAATTTTATTTTGTTCCACTTTGTGTTTACGAGGATGAAGATAGAACCATATGA", "aa": "MGIPKEDIIDFFKLDPSVFDDFIFLMRVYNEAMYLNDYSKNNSKSLSPFDSKSLYSNKDFLELNRFIVQKNHNNFDNKLNISKIEKFYFVPLCVYEDEDRTI", "aa_hexdigest": "36e512004936d33f465d66cfe0657b23", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12244.743599999987, "isoelectric_point": 4.881014442443848 }, "id": "DFOKDJOJIG_969", "locus": "GCA013367855_00961" }, { "type": "cds", "sequence": "contig_1", "start": 545681, "stop": 545833, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATGTCCAAAAGACTTTTATAAATATAGAATTATTGTTCACTTTACAGCATATTTATATAATAAATTATTAAACACTGATTTTAACGCTGCTAAGGTTAACACTAAAAGAATAAATGAAATATTTGAAAAGTTAAACATTAAAGACTAA", "aa": "MICPKDFYKYRIIVHFTAYLYNKLLNTDFNAAKVNTKRINEIFEKLNIKD", "aa_hexdigest": "4d6d525a10d2f40f07be44431cd0d210", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6072.0634, "isoelectric_point": 9.416461753845216 }, "id": "DFOKDJOJIG_970", "locus": "GCA013367855_00962" }, { "type": "cds", "sequence": "contig_1", "start": 545844, "stop": 546326, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MHO_3790", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43056", "UniRef:UniRef90_P43056" ], "nt": "ATGAAAAAAGGCAAAGCAAAAGAAACAAAAACTATTGATGTCGACAAGCAAGAAAAAAAATCAATTAGAATTCGTAGAGTAAAAACCGGATTTCTATTTTCAGGAATTTTAATTCCTTTAGTTTGCGTTGTCGCAATACCACTTTCATTAAACAAGAATTATTCATTAAAGCGAACCAAATATTTTACTGACGATAGAACTTTATATTCAATTGACAAAAACACTATAAAAGAAAATAATAAAACTTATAAAATACTTACCCTAACATCTAAAAGTCTTAATGTTTCTGATATCATGAGCGAAAGCGCAAGATATATGAATATTCCACAAAAATCATTTAATAATTCCGTTACATATTCAAATAAAATCAATATTAGAACAAACACCAACTTGTTAAAAAATACAAAAATTAATGAGACAAAGGTTACAAAAATAACTAATAGTAACCAAACTATTTTTAAATTAGATTTAGTTCAACTATGA", "aa": "MKKGKAKETKTIDVDKQEKKSIRIRRVKTGFLFSGILIPLVCVVAIPLSLNKNYSLKRTKYFTDDRTLYSIDKNTIKENNKTYKILTLTSKSLNVSDIMSESARYMNIPQKSFNNSVTYSNKINIRTNTNLLKNTKINETKVTKITNSNQTIFKLDLVQL", "aa_hexdigest": "77f65b69dd86c90398d63d30ba94eb2f", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 545844, "stop": 546629, "observations": { "mutation": [ 546324 ], "stop": [ 546324 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43056", "query_cov": 1.0, "subject_cov": 0.5970149253731343, "identity": 0.975, "score": 290.0, "evalue": 3.89e-97, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MKKGKAKETKTIDVDKQEKKSVRIRRVKTGFLFSGILIPLVCVVAIPLSLNKNYSLKRTKYFTDDRTLYSIDKNTIKENNKTYKVITLTSKSLNVSDIMSESARYMNIPQKSFNNSVTYSNKINIRTNTNLLKNTKINETKVTKITNSNQTIFKLDLIQLWNEINSKSNNKISEFDFLSNIESINNAMTYSYIKNNKLFYFDNKQGYSGYIIQKFFENFDVEIRKENLKNKNVNVLEAFISSTIELNESLTEIKKITFDTKITLKYEI" }, "paralog": false, "identity": 0.9731800766283525, "score": 499.0, "evalue": 3.31e-182, "description": "Internal stop codon at 546324. Nonsense mutation around 546324.", "psc": { "uniref90_id": "UniRef90_P43056", "product": "Uncharacterized protein MHO_3790", "uniref50_id": "UniRef50_P43056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43056", "UniRef:UniRef50_P43056" ] }, "pscc": { "uniref50_id": "UniRef50_P43056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43056" ], "product": "Uncharacterized protein MHO_3790" } }, "id": "DFOKDJOJIG_971", "locus": "GCA013367855_00963" }, { "type": "cds", "sequence": "contig_1", "start": 546405, "stop": 546650, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTACTCTTATATAAAAAATAACAAACTCTTTTACTTTGACAATAAGCAAGGATATTCTGGATATATTATTCAAAAGTTCTTTGAAAATTTTGATGTTGAGATTCGTAAGGAGAATCTTAAAAATAAAAATGTTAATGTTTTAGAAGCTTTTATTTCTAGCACTATTGAATTAAACAAATCATTAACCGAAATCAAAAAAATCACTTTTGATACTAAAATTACATTAAAATATGAAATATAA", "aa": "MTYSYIKNNKLFYFDNKQGYSGYIIQKFFENFDVEIRKENLKNKNVNVLEAFISSTIELNKSLTEIKKITFDTKITLKYEI", "aa_hexdigest": "1b0ac4ee1f4e4857d63dce943a1452c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9720.067399999989, "isoelectric_point": 9.09856815338135 }, "id": "DFOKDJOJIG_972", "locus": "GCA013367855_00964" }, { "type": "cds", "sequence": "contig_1", "start": 546693, "stop": 547112, "strand": "-", "frame": 2, "gene": null, "product": "DUF885 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "nt": "ATGTTACAATACTTTGGAGCATTAAACGAAGCACAATTACGTAATATGCGTAGAGCAGTTGACCCTGCATACCATGGACAAGGAATTGAAGCAAAATCTGATTTACCTGCAAATGCATCAATTAATGATGTAAGAAGATTTATGTCAGCAAACTCAGCATTAGGTGTTGGTGACATTATGTCTGAATCAAAACGTTACCTAAATCTAGCTGGACAAGCAATTTCATACAATGCTGGTAAAGAAAAGATGCTATCTCTATACGACCAAGTACGTAAACACTTAGGACTATCAAGAAGAGACTTTGTTGAAAAAGAAAACCACAAATACATAAAAGAATTCTTTGACGTTCTATTGAAGAATAGTGCCTTACCTCTAGATACTTTAGAAAAAGTTACAAAGATTCACTACGGTATAAAATAA", "aa": "MLQYFGALNEAQLRNMRRAVDPAYHGQGIEAKSDLPANASINDVRRFMSANSALGVGDIMSESKRYLNLAGQAISYNAGKEKMLSLYDQVRKHLGLSRRDFVEKENHKYIKEFFDVLLKNSALPLDTLEKVTKIHYGIK", "aa_hexdigest": "18a64166ce19bbb7dabfe5108129b1e1", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A3B0P8H0", "query_cov": 0.9424460431654677, "subject_cov": 1.0234375, "identity": 0.611, "score": 164.0, "evalue": 4.8e-50, "valid": false, "product": "Bacterial protein of uncharacterized function (DUF885)", "uniref50_id": "UniRef50_A0A3B0P8H0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "product": "Bacterial protein of uncharacterized function (DUF885)" }, "genes": [], "id": "DFOKDJOJIG_973", "locus": "GCA013367855_00965" }, { "type": "cds", "sequence": "contig_1", "start": 547386, "stop": 547493, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACACCACAACCAAATTTACTACGCACGTCAACACCTAGCAAGTCAAAACAATGAAAATCTAGGTGCAAATGCATTTGACTTTACTTCATACGTTGAAGGTTGA", "aa": "MGHHNQIYYARQHLASQNNENLGANAFDFTSYVEG", "aa_hexdigest": "73304a03f826e55bdef9d8c1b31f17be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3998.2259000000004, "isoelectric_point": 5.727915382385254 }, "id": "DFOKDJOJIG_974", "locus": "GCA013367855_00966" }, { "type": "cds", "sequence": "contig_1", "start": 547527, "stop": 547736, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTCAACCATCAAGAATGGAAAATCATTCTTCGGTAAAACTGTTCCTGACTACAAAGCAGTAACATATCTATATGAAAGACGTGATCAAGAAGGTGTTGGAGCATACAACTCAGATACACGTATTTTGGATGCAAACGGAAATGTAACTTCTGTTCAAGGTGCATTTAACTTCAACTGTGACCCATACTACTCATTGCCAAAATGA", "aa": "MKSTIKNGKSFFGKTVPDYKAVTYLYERRDQEGVGAYNSDTRILDANGNVTSVQGAFNFNCDPYYSLPK", "aa_hexdigest": "39ee82c016e2bc1fcb674a55993c58f5", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7752.532500000003, "isoelectric_point": 8.75869083404541 }, "id": "DFOKDJOJIG_975", "locus": "GCA013367855_00967" }, { "type": "cds", "sequence": "contig_1", "start": 548082, "stop": 548414, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAGAAAATGTTTACAAAGAAACAGAAAACACAATATCAGTTTCAAAACAAGAAGACGGAAAACTTATTGAAAGATCAGTTTATGGTTTAGGTTTTACCAAATTTGACTTAGAAGCTAAGGGCGTTGGATTCTCACAAGTTGGCGGTGATGAAACTGAAAGTGGAAAGACCATTTATGACCAAATCACAAAAATGGCAACCACATCAGAAGACACTCCAAACGATGTATATCAATCAGGATACAAAACATCAAAAGGTGTTTCAAAGAACATGACTGCCATTGCATCAGAAGTTGCTAAATTAATAGCTGGTGAAAGTGGAGAATGA", "aa": "MYKENVYKETENTISVSKQEDGKLIERSVYGLGFTKFDLEAKGVGFSQVGGDETESGKTIYDQITKMATTSEDTPNDVYQSGYKTSKGVSKNMTAIASEVAKLIAGESGE", "aa_hexdigest": "a3597d609de2ea04fcbdeaa15649c57f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11938.052199999991, "isoelectric_point": 4.809226799011231 }, "id": "DFOKDJOJIG_976", "locus": "GCA013367855_00968" }, { "type": "tRNA", "sequence": "contig_1", "start": 549687, "stop": 549775, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 65.9, "nt": "GGATAGTTGCTCGAGAGGCTGAAGAGGTTAGTCTCGAAAACTAATAATGTTTTTAACATTCAAGGGTTCAAATCCCTTACTATCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 549762, 549764 ], "id": "DFOKDJOJIG_26", "locus": "GCA013367855_00969" }, { "type": "cds", "sequence": "contig_1", "start": 550035, "stop": 551321, "strand": "+", "frame": 3, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537", "KEGG:K15633", "SO:0001217", "UniRef:UniRef50_Q8EW33", "UniRef:UniRef90_Q6Y8Q8" ], "nt": "ATGAAAAAAATAATTCTTACAATTATCGATGGACTTGGTCTAAGAAAAGAAAGGCAAGGAAATGCTTATTTACAAGCAAAACACCCTTGCTTTGACTATCTTTTTTCAATGTGCCCTAATTCTGTTTTACAAGCATCTGGTCAATACGTTGGTTTGCCAGAGGGGCAAATTGGCAATAGCGAAGTAGGACATTTAAATATTGGCGCAGGTAGAGTTGTATATACGGGATTATCTTTAATTAATAAGGCAATTGAAAATGATACTTTTAAGGATAATGAAATTTTAAATGATGTCATAGATAAAACTATTAAAAATAATACAACATTGCATGTAATGGGTTTATTAAGCAACGGCGGTGTTCATTCATTAGATTTACATTTGTTTGAAATTCTAAAATTAGCGCATAGTAAAGGATTAAAAAACGTAAGTGTTCATGTGTTTGGTGATGGAAGAGATGTAAAACCACAATCAATAAAAAATTCTCTTGAAACCTTAAAAGATTTATGCCAAAAATTTGGATATAAAATCTCTTCTATATCTGGCAGGTTTTATGCAATGGATAGAGATAGTATATTTTCAAGAAATCAAGAAGCATATGATGCCATTTTAGGGCAATCAAAAAATGTTATTGAAAACATTGATGACTACATTGAAAGTCAATATAAAAAAGGAATATTTGACGAATTTTTTGAGCCTGCTCAATTAAAAGACGGCGTATTTGTTAAAAATGGAGATTCGATTATATTCTTTAACTTTAGACCAGATAGAGCAAGACAGCTAAGCCATATGTTTATTGGATCAAACTTATACACATACAAACCAAGAAACCAAGTACAAATTGATAATTTTGTTTCATTGATGAAATATGAAGGTATAAATAGTAAAATAGCCTTTAAAGAAATGGAAGTTGTAAATCCACTAGGCAAGGTTTTAGAAAATAATGACATTAAACAATTAAGACTTGCAGAAACTCAAAAATATGCCCATGTAACATTCTTTTTTGATGGAGGGGTTGACATCGAATATAAAAATGAAAATAGAATTCTAGTTGATAGTCTTAAGGTTGATTCATTTGCTGATTATCCACATATGTCGGCTAAAGAAATTACAGATTCACTATTGAACAACATTGAAAAAAATGATTTTATAATAATGAATTATGCAAATCCTGATATGGTGGGCCATACCGGTAATTTGAACGCAACAATTGAAGCAATTGAATTTTTAGATTCACAATTTCAAAGAATATTAGAATATGTGTCATTAAATCATGAAAATGTAACCTGA", "aa": "MKKIILTIIDGLGLRKERQGNAYLQAKHPCFDYLFSMCPNSVLQASGQYVGLPEGQIGNSEVGHLNIGAGRVVYTGLSLINKAIENDTFKDNEILNDVIDKTIKNNTTLHVMGLLSNGGVHSLDLHLFEILKLAHSKGLKNVSVHVFGDGRDVKPQSIKNSLETLKDLCQKFGYKISSISGRFYAMDRDSIFSRNQEAYDAILGQSKNVIENIDDYIESQYKKGIFDEFFEPAQLKDGVFVKNGDSIIFFNFRPDRARQLSHMFIGSNLYTYKPRNQVQIDNFVSLMKYEGINSKIAFKEMEVVNPLGKVLENNDIKQLRLAETQKYAHVTFFFDGGVDIEYKNENRILVDSLKVDSFADYPHMSAKEITDSLLNNIEKNDFIIMNYANPDMVGHTGNLNATIEAIEFLDSQFQRILEYVSLNHENVT", "aa_hexdigest": "d010dff4687f73ea3a3b1ccd999552df", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q6Y8Q8", "query_cov": 1.0, "subject_cov": 0.8577154308617234, "identity": 0.993, "score": 843.0, "evalue": 8.66e-308, "valid": true, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_Q8EW33", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EW33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EW33" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "DFOKDJOJIG_977", "locus": "GCA013367855_00970" }, { "type": "cds", "sequence": "contig_1", "start": 551406, "stop": 551534, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTTGCACAGATAAAAATGTTAATTTAGAAAATGGTAGTCTTTGTGATGTAGCTCCTACAATATTAGATTATTTAAAAATCAACAAACCAAAAGAAATGACTGGAAAATCATTACTTAAATAA", "aa": "MFICTDKNVNLENGSLCDVAPTILDYLKINKPKEMTGKSLLK", "aa_hexdigest": "3b357b54df9d82f8863eb3d8e8dd0ac8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4699.5321, "isoelectric_point": 7.761273384094238 }, "id": "DFOKDJOJIG_978", "locus": "GCA013367855_00971" }, { "type": "cds", "sequence": "contig_1", "start": 551576, "stop": 552064, "strand": "-", "frame": 3, "gene": null, "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZZ8" ], "nt": "TTGCAAGATACATTTCTATTTAAAACAACAATTTTAGAAAATCTAAGATATGCTAATCCTAGTGCAAGCAATGAAGAAATCATTAATGCTGCCAAAATTACAAAGGCCGATAATTTTATAAGACAACTTGAACATGGTTACGAAGAAATAGTTGAAGAAGGCGGAAGCAATTTTTCACAAGGTGAAAGACAGATGCTTGCAATCACAAGAGCAATAATTGCTAATAAAAATATTTTGATATTAGACGAAGCAACATCTAATGTAGATACAAGAACAGAAAAGAATATTCAAGATGCAATGTTAGAGTTAATGAAAGGAAAAACTTCATTTGTTATTGCTCATAGACTTTCAACTATAGTAAATGCTAGCCAAATTTTAGTTGTTAAAGATGGAAAAATTATTGAACAAGGTACACACAAAGAACTATTGACTAAAGGTAGTTTTTATGAAAAGCTATATCATTCATCTTTTAGTGAAGAAAATGAATAA", "aa": "MQDTFLFKTTILENLRYANPSASNEEIINAAKITKADNFIRQLEHGYEEIVEEGGSNFSQGERQMLAITRAIIANKNILILDEATSNVDTRTEKNIQDAMLELMKGKTSFVIAHRLSTIVNASQILVVKDGKIIEQGTHKELLTKGSFYEKLYHSSFSEENE", "aa_hexdigest": "c4f9e9a000d7b0695385d06c1f095888", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NZZ8", "query_cov": 0.9567901234567902, "subject_cov": 1.0, "identity": 0.735, "score": 229.0, "evalue": 5.29e-75, "valid": false, "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component", "uniref50_id": "UniRef50_A0A3B0NZZ8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NZZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZZ8" ], "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component" }, "genes": [], "id": "DFOKDJOJIG_979", "locus": "GCA013367855_00972" }, { "type": "cds", "sequence": "contig_1", "start": 552086, "stop": 553147, "strand": "-", "frame": 3, "gene": "mdlB", "product": "ABC transporter ATP binding protein", "db_xrefs": [ "COG:COG1132", "COG:V", "SO:0001217", "UniRef:UniRef50_A0A448ZXF5", "UniRef:UniRef90_D1J8H0" ], "nt": "TTGATCTTAGGCTATATTTTAGAAAAAGTCTTTTCATTTATATGTTCAGCAATGGCAGTCAAGGCAGGAATTTATGGCTCAGAAATAATGCGGAGTAAGGCTTATAAATCAATAATGAAAATGCCAATCCAATTTTTTGATACTGTTAGCTCTGGAGAATTAATGTCAATATTGTCAAATGATATTGATAATGTTTCATATGGTATATGTGGAACCTTAAACTCGTTATTAGCAGCGATATTTAGTGCTTTAACATCGCTTGGATTTATGTTTTACTATTCTACATATTTAACACTTATATATATTGCCTTATTTCCTGTTTTGTTGATATTGGCTTTTGTTCTAATTAAAAAAGCAATTCCATTATTTGGCAAGACTCAAAGAAATATAGGTTTATTGAATGGTTTTGTTGAAGAAAATTTAGCGGCACACCAATTAATAAATTCATTGAATGGTCAACAAATTATTAACGAACAATTTGATTTATATAATGAAAAATTATATAAACCTTCATTAAAAGCAAATATATATGCCGGAATAGTTTATCCATATGGAAATGCAATAACATTCATAATGCAATTAATAATAATTTTTGTTGGCGGAGCATTTGCATTGTCTGGAATTGGCACTGGCTCAAATAGTAATTTTACAGTCGGTACATTGACATCATTTATTTTGTATATAAAAATTACTTCTAGTCAAATAATTAAAAATTTTGAAAATATTTCAATGATTCAAACAGCAACAATATCAACAACAAGAGTATTAAATTTAATTAGAATGCATCCGGCCGTTGATGAAAGCAAACTTGCTACAATATCAAATGTGAATGGTGATATTGAATTTAAAAATGTAAGTTTTTCATATGATAATTCTTTAAATAATTTACAATTGAAAAATATTTCATTCAAAGCAAAAAAAGGTCAAGTATTTGCTATTGTAGGACCTACCGGGGCAGGCAAAACTACAATAATAAATTTACTAAGCAAATTTTATTTGCCTATTGAAGGGGAAATAAAAATTGATGATTATAAATCAAGCGAAATAAATGAATCTAGTTGA", "aa": "MILGYILEKVFSFICSAMAVKAGIYGSEIMRSKAYKSIMKMPIQFFDTVSSGELMSILSNDIDNVSYGICGTLNSLLAAIFSALTSLGFMFYYSTYLTLIYIALFPVLLILAFVLIKKAIPLFGKTQRNIGLLNGFVEENLAAHQLINSLNGQQIINEQFDLYNEKLYKPSLKANIYAGIVYPYGNAITFIMQLIIIFVGGAFALSGIGTGSNSNFTVGTLTSFILYIKITSSQIIKNFENISMIQTATISTTRVLNLIRMHPAVDESKLATISNVNGDIEFKNVSFSYDNSLNNLQLKNISFKAKKGQVFAIVGPTGAGKTTIINLLSKFYLPIEGEIKIDDYKSSEINESS", "aa_hexdigest": "80504d85ccf55d29f8e49ecacd4e0fef", "start_type": "TTG", "rbs_motif": null, "genes": [ "mdlB" ], "truncated": "both", "pseudogene": { "start": 551783, "stop": 553447, "observations": { "mutation": [ 552088, 553198 ], "stop": [ 552088, 553198 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H0", "query_cov": 1.0, "subject_cov": 0.5648, "identity": 0.992, "score": 652.0, "evalue": 1.25e-231, "gene_start": 1, "gene_end": 353, "reference_start": 103, "reference_end": 455, "reference_sequence": "MNRDILDPLKVDIDLKKVSKFERKAFRKKQHAIKSESFKELIKYLNYKRGYVFLIIIITLISSLCLTAATYGLGYIIDHYLNFKFWNSNSNGLFEFGIAIGILILGYILEKVFSFICSAMAVKAGIYGSEIMRSKAYKSIMKMPIQFFDTVSSGELMSILSNDIDNVSYGICGTLNSLLAAIFSALTSLGFMFYYSTYLTLIYIALFPVLLILAFVFIKKAIPLFGKTQRNIGLLNGFVEENLAAHQLINSLNSQQIINEQFDLYNEKLYKPSLKANIYAGIVYPYGNAITFIMQLIIIFVGGAFALSGIGTGSNSNFTVGTLTSFILYIKITSSQIIKNFENISMIQTATISTTRVLNLIRMHPAVDESKLATISNVNGDIEFKNVSFSYDNSLNNLQLKNISFKAKKGQVFAIVGPTGAGKTTIINLLSKFYLPIEGEIKIDDYKSSEINESSWRNQISIVLQDTFLFKTTILENLRYANPSASNEEIINAAKITKADNFIRQLEHGYEEIVEEGGSNFSQGERQMLAITRAIIANKNILILDEATSNVDTRTEKNIQDAMLELMKGKTSFVIAHRLSTIVNASQILVVKDGKIIEQGTHKELLTKGSFYEKLYHSSFSEENE" }, "paralog": false, "identity": 0.9927797833935018, "score": 1055.0, "evalue": 0.0, "description": "Internal stop codon at 552088, 553198. Nonsense mutation around 552088, 553198.", "psc": { "uniref90_id": "UniRef90_D1J8H0", "gene": "MdlB", "product": "ABC transporter ATP binding protein", "uniref50_id": "UniRef50_A0A448ZXF5", "cog_id": "COG1132", "cog_category": "V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H0", "UniRef:UniRef50_A0A448ZXF5", "COG:COG1132", "COG:V" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXF5" ], "product": "ABC-type multidrug/protein/lipid transport system ATPase component" } }, "id": "DFOKDJOJIG_980", "locus": "GCA013367855_00973" }, { "type": "cds", "sequence": "contig_1", "start": 553196, "stop": 553453, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGAGATATTCTTGATCCGCTAAAAGTTGATATTGATTTAAAAAAAGTATCTAAATTTGAAAGAAAAGCATTTCGAAAAAAACAACACGCAATAAAATCAGAAAGTTTTAAAGAACTAATTAAATATTTAAATTACAAACGTGGATATGTTTTTTTAATAATAATAATTACATTAATTTCTTCATTGTGTTTAACTGCTGCCACATATGGTTTGGGCTATATTATTGACCATTATTTAAACTTTAAGTTTTGA", "aa": "MNRDILDPLKVDIDLKKVSKFERKAFRKKQHAIKSESFKELIKYLNYKRGYVFLIIIITLISSLCLTAATYGLGYIIDHYLNFKF", "aa_hexdigest": "a80f3f94ab1456aec97f2b99dee99346", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10062.88029999999, "isoelectric_point": 9.714434623718262 }, "id": "DFOKDJOJIG_981", "locus": "GCA013367855_00974" }, { "type": "cds", "sequence": "contig_1", "start": 553446, "stop": 554348, "strand": "-", "frame": 2, "gene": null, "product": "Multidrug ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D3WBZ0" ], "nt": "ATGAATTACGTTATGACTGTTACATTTGGAGTTGTTGCTTCATCATTTACTACATTTAATATTTTCAAGGCAAATGTATCCTCAAAACGTATTTTTGAAATTATGAATAAAAAGCCAGATATTGCAAAAATCAAATCTGATAAGCTAATTGTTAATGGAGAAATTGAATTTAGCCATGTATCTTTTAAATATTATGAATCTGCCAAATCTAATGTTTTAGAAGATATTTCATTTAGTTTAAAGCCTGGTAAGGTTTTAGGCATAATTGGCCCTACTGGTTCTGGAAAATCAACTATTGCAAAATTGTTAAATTTAGATTTTAAAACTCAAGACGGGCTAGTAACAATTGATGGGCATAATATTCAAGAAATTGATACAGACTCATTGAGAAAAAATATCTCCCACGTTTATCAAAACCCTTGCTTATTAAGTGGCACAATAAAAAGCAATTTATTGCTTGCTAAACCTAATGCCACTGATGAAGAGATTGAATTAGCAGCAAAAAATGGTTGCGCTTTTGAATATATCAATAAGTTTAAAGAAAAATTTGAACATGTTGTTGAACAAAAGGGTGCAAATTTATCAGGTGGTCAAAAACAAAGATTATCTATTGCCCAAGGTTTGATTAAACGACCTAAAATATTGATTTTAGATGATTCTACTTCAGCATTGGATGCTAGAACAGAAGCTATGGTAAGAAACAATATTAAAGAAGAATTTAAATACGAAAAAATATCATTAGTTATTATTGCCCAAAAAATTTCAGCAATTATTGATGCAGATGAAATTTTAGTATTAAATCAAGGAAAAATTATTGATCGTGGCAATCATGAGGAATTAATTTCTAGAGATGGTCTATATAAAGAAATTGCATATTCACAGTTAGGGGGTAATAATGAATAG", "aa": "MNYVMTVTFGVVASSFTTFNIFKANVSSKRIFEIMNKKPDIAKIKSDKLIVNGEIEFSHVSFKYYESAKSNVLEDISFSLKPGKVLGIIGPTGSGKSTIAKLLNLDFKTQDGLVTIDGHNIQEIDTDSLRKNISHVYQNPCLLSGTIKSNLLLAKPNATDEEIELAAKNGCAFEYINKFKEKFEHVVEQKGANLSGGQKQRLSIAQGLIKRPKILILDDSTSALDARTEAMVRNNIKEEFKYEKISLVIIAQKISAIIDADEILVLNQGKIIDRGNHEELISRDGLYKEIAYSQLGGNNE", "aa_hexdigest": "b376c7e2146eb1cab9d1431a53a9542f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031CC58F0", "query_cov": 0.8, "subject_cov": 0.9876543209876543, "identity": 0.5, "score": 212.0, "evalue": 9.32e-65, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A3D3WBZ0" }, "pscc": { "uniref50_id": "UniRef50_A0A3D3WBZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D3WBZ0" ], "product": "Multidrug ABC transporter ATP-binding protein" }, "genes": [], "id": "DFOKDJOJIG_982", "locus": "GCA013367855_00975" }, { "type": "cds", "sequence": "contig_1", "start": 554910, "stop": 555269, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter transmembrane domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241122C8", "UniRef:UniRef90_UPI00241122C8" ], "nt": "ATGTTTAAACTTTATAAATTAATGGCCAACAAATTCAAATGTTTGTCATTTTTAACTATATCTTTAACATTGCTACAAGTAGTTTCATTTTTAGTATTGCCAATATTATTAGGACAATTGACAAGGTTAATTGGTGAAAATGCATATTTGATTCAAAATAATTTGTCAACAAACAGACCAATAACAATTGAAATTCTAAGAATTAATTTTTTATGTCAAAGCCACCAGAGTGCGTTAATGCATTTGGGAGGATATTTTGCATTGTTTTTAATAATAGGAACTATTTCAGCAATGTGTGCTTCATTGCTTGCATCATATGTGTCTCAATCAGGATCAAAACAAATAAGAAGTTGTTTATGA", "aa": "MFKLYKLMANKFKCLSFLTISLTLLQVVSFLVLPILLGQLTRLIGENAYLIQNNLSTNRPITIEILRINFLCQSHQSALMHLGGYFALFLIIGTISAMCASLLASYVSQSGSKQIRSCL", "aa_hexdigest": "d70fcd828efe2a465e374b96f169c38f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 554609, "stop": 555269, "observations": { "insertion": [ 554645 ], "mutation": [ 554660, 554912 ], "stop": [ 554660, 554912 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00241122C8", "query_cov": 1.0, "subject_cov": 0.5265486725663717, "identity": 0.992, "score": 216.0, "evalue": 1.44e-69, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MFKLYKLMANKFKCLSFLTISLTLLQVVSFLVLPILLGQLTRLIGENAYLIQNNLSTNRPITIEILRINFLCQSHQSALMHLGGYFALFLIIGTISAMCASLLASYVSQAGSKQIRSCLWKHLGELSQKDIEAFSNAKILTRFTIDISRIQTGLMSFLRMLIIGPFNLVLGLVFALLTNLQLSMIFLVVIPLLTLTMVISGVIWNPIQKKNKKCMTKLTLKVEKIF" }, "paralog": false, "identity": 0.9863013698630136, "score": 404.0, "evalue": 5.97e-146, "description": "Internal stop codon at 554660, 554912. Frameshift due to insertion around 554645. Nonsense mutation around 554660, 554912.", "psc": { "uniref90_id": "UniRef90_UPI00241122C8", "product": "ABC transporter transmembrane domain-containing protein", "uniref50_id": "UniRef50_UPI00241122C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00241122C8", "UniRef:UniRef50_UPI00241122C8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00241122C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241122C8" ], "product": "ABC transporter transmembrane domain-containing protein" } }, "id": "DFOKDJOJIG_983", "locus": "GCA013367855_00976" }, { "type": "cds", "sequence": "contig_1", "start": 555318, "stop": 555731, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATGAAGAATCAGGATTATTGGGAGTTTCAAAAATTTCTTCTGATATTCGTGACGTTGAAGAAGCTAAAAAAACAAATGAAAGAGCAAAATTTGCAATTGATTTATATGTAAGAAAAATAGCAGATTATTTAATTAATTATATGAACAAGATTGAAGGAAAAATCGATGCTATTGTTTTTACGGCGGGTGTTGGGGAAAATGATGAATATATTCGCCAAGCAGTTATTGATAAAATTCATTTAGTTAAATTAGAAATTGATAGTGAACTAAATAAAGGAAGAGATTTTGATTCATACAAATTAATTTCAACAAAAAACTCTTCAATTCCTATTTATATAATAAGGGCAGATGAAGAACTTTATATCGCAAAAGAAGTTAAACAATTTTTTGATGATAATACAAGGTAA", "aa": "MLNEESGLLGVSKISSDIRDVEEAKKTNERAKFAIDLYVRKIADYLINYMNKIEGKIDAIVFTAGVGENDEYIRQAVIDKIHLVKLEIDSELNKGRDFDSYKLISTKNSSIPIYIIRADEELYIAKEVKQFFDDNTR", "aa_hexdigest": "c420761faf6c4fb42f15c171216a70d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15755.733699999986, "isoelectric_point": 5.012255668640137 }, "id": "DFOKDJOJIG_984", "locus": "GCA013367855_00977" }, { "type": "cds", "sequence": "contig_1", "start": 555753, "stop": 556097, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTCCACTTTGATACTTCATTCCATAAAACCCTTCCAAAAGAAGCATATACTTATGCAATTGATGCAAATATAACCGATGAATTAAATGTTAGAAAATATGGTTTCCATGGCTTGAATCACCACTTTATTACATTAAAATTGCAAGAAATTTTAAATAAAGACAAAGTTAATTTTGTTAATTTACATATCGGAAATGGCGCAAGTTTAGCAGCCATTCAAGATAGTAAATCAATTGATACCTCAATGGGATTTACACCACTAGCGGGAGTTATGATGGGCACTCGTTCTGGTGATGTTGATTGTTCAATTATTCCATATATTATGAAACATAAAAATTGA", "aa": "MTFHFDTSFHKTLPKEAYTYAIDANITDELNVRKYGFHGLNHHFITLKLQEILNKDKVNFVNLHIGNGASLAAIQDSKSIDTSMGFTPLAGVMMGTRSGDVDCSIIPYIMKHKN", "aa_hexdigest": "bf9ea4f0f79cb2cb311df2ef9af91b04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12773.492099999985, "isoelectric_point": 7.055731010437011 }, "id": "DFOKDJOJIG_985", "locus": "GCA013367855_00978" }, { "type": "rRNA", "sequence": "contig_1", "start": 556602, "stop": 556706, "strand": "-", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8823529411764706, "score": 60.7, "evalue": 1.3e-09, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "CGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCCTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTT", "id": "DFOKDJOJIG_38", "locus": "GCA013367855_00979" }, { "type": "cds", "sequence": "contig_1", "start": 556809, "stop": 557006, "strand": "-", "frame": 2, "gene": null, "product": "Smr domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN26", "UniRef:UniRef90_D1J8H3" ], "nt": "ATGAAGTTCAATTTTTTTCCAATGGTGTTAGATCTTCATGGCTATGACGTAAACTCTGCAACAGCAGCAATTTTAAACGCTTTGTATGAATTTAAACAAGATGAATATAATAATTACTTTGATATTATTGTAGGCATTGGAAGTGGGATTATTCGCCAAATAACAGAAGACTTATTAGACAAAGAAGGATATTATTGA", "aa": "MKFNFFPMVLDLHGYDVNSATAAILNALYEFKQDEYNNYFDIIVGIGSGIIRQITEDLLDKEGYY", "aa_hexdigest": "ea0ebfb1e6a408a77567f0648b2a77c4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 556761, "stop": 557006, "observations": { "mutation": [ 556811 ], "stop": [ 556811 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H3", "query_cov": 1.0, "subject_cov": 0.7926829268292683, "identity": 0.985, "score": 135.0, "evalue": 2.25e-40, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MKFNFFPMVLDLHGYDVNSATAAILNALYEFEQDEYNNYFDIIVGIGSGIIRQITEDLLDKEGYYWKNIGNSLAKIRVFKKI" }, "paralog": false, "identity": 0.9753086419753086, "score": 160.0, "evalue": 6.3e-53, "description": "Internal stop codon at 556811. Nonsense mutation around 556811.", "psc": { "uniref90_id": "UniRef90_D1J8H3", "product": "Smr domain-containing protein", "uniref50_id": "UniRef50_B3PN26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H3", "UniRef:UniRef50_B3PN26" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN26" ], "product": "Smr domain-containing protein" } }, "id": "DFOKDJOJIG_986", "locus": "GCA013367855_00980" }, { "type": "cds", "sequence": "contig_1", "start": 557007, "stop": 557870, "strand": "-", "frame": 2, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "EC:3.2.2.23", "EC:4.2.99.18", "GO:0005737", "GO:0006281", "GO:0008534", "GO:0046872", "KEGG:K10563", "SO:0001217", "UniRef:UniRef50_A0A9Q9F2U2", "UniRef:UniRef90_A0A454C9Y4" ], "nt": "ATGAGTAATGTCATTCGAAAGTATTATAATTTTACTATGCCTGAATTACCTGAAGTTAGGGTTGTTGTTAAAGCCCTTAATAATACTATATTAAATAAAAAAATAACAAATTTGATTATATACAAACCCAAAATTTTTAAAGAGTTTAGCCCTCAATATTTCATTTCAATTTTAAGAGATAAAAAAATTGAAAAAATAGATAACATTGGCAAACATATAATTTTCTTTTTATCAGAAAAATTAGTTTTACTTTCTCATTTAAGAATGGAAGGAAAATATCGTTATTATTTAAACCCCCCAAAAGAAATTGATAAGCACTTAGTTGCTAGATTTGTTTTTAGCGACAACAGCGAATTGCGCTTTTTAGATAAACGTTTATTTGGCACATATATGCTAAGAAACTTAGAAAATTATAATAAAATTCCTCCAATTTCTTTGCTTGGACCTGAGCCAAAGGATATTGACATTGAAGCATTATTTAAAAAAATAAAAAATTCAAAAATGCCCATTAAAACAAAATTACTTGATCAGAGTTTTGTTGCTGGTATAGGAAATATTTACGCTGATGAAGCATTGTTTGCTTCCAAAATTCATCCTCTTTCAAAAGCTTCTGATTTATCACTAGAGCAACTTAAAGACATAATAAAAAATGCAAATGAAATAATGGAAAAAAGTTATGAATTAGGTGGAACAACGTTATTTTCTTATGAATCATTAAATAAACAAGAAGGAAAATATCAAGACTTTTTAAATATTCATAGTGATACTATAAAAAAATGTAAGGTTTGTGGTCAAAAAACACTTAAATTAAAAGTTAACCAACGTGGTACATATGTTTGCCCTAATTGTCAGAAGGTATATTAA", "aa": "MSNVIRKYYNFTMPELPEVRVVVKALNNTILNKKITNLIIYKPKIFKEFSPQYFISILRDKKIEKIDNIGKHIIFFLSEKLVLLSHLRMEGKYRYYLNPPKEIDKHLVARFVFSDNSELRFLDKRLFGTYMLRNLENYNKIPPISLLGPEPKDIDIEALFKKIKNSKMPIKTKLLDQSFVAGIGNIYADEALFASKIHPLSKASDLSLEQLKDIIKNANEIMEKSYELGGTTLFSYESLNKQEGKYQDFLNIHSDTIKKCKVCGQKTLKLKVNQRGTYVCPNCQKVY", "aa_hexdigest": "4700b08c55b4bebb303d4e0fa8842689", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454C9Y4", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.997, "score": 568.0, "evalue": 9.66e-205, "valid": true, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23", "4.2.99.18" ], "uniref50_id": "UniRef50_A0A9Q9F2U2", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0005737", "GO:0006281", "GO:0008534", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2U2" ], "product": "DNA-(apurinic or apyrimidinic site) lyase" }, "genes": [ "mutM" ], "id": "DFOKDJOJIG_987", "locus": "GCA013367855_00981" }, { "type": "cds", "sequence": "contig_1", "start": 557912, "stop": 558088, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAATAAATATCAAAAATTAATTATTTCAACTACAGACACAGTTTTAGGAATTGGTGGCCCTGTGTCAAAAGAAGTTGAAGACCTTATTTATGAAATAAAAGGTAGAGACCGCAATAAAAAATTAATAATATTAGTAAGTTCAATAAAACAAGCAAGGCAATTTAAGGAATGA", "aa": "MINKYQKLIISTTDTVLGIGGPVSKEVEDLIYEIKGRDRNKKLIILVSSIKQARQFKE", "aa_hexdigest": "fffbfe4be100210b6dc72de2222d76bc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6619.7276, "isoelectric_point": 9.629852104187012 }, "id": "DFOKDJOJIG_988", "locus": "GCA013367855_00982" }, { "type": "cds", "sequence": "contig_1", "start": 558167, "stop": 558379, "strand": "+", "frame": 2, "gene": "tsaC", "product": "L-threonylcarbamoyladenylate synthase", "db_xrefs": [ "COG:COG0009", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A077L684", "UniRef:UniRef90_D1J8H5" ], "nt": "ATGCCAAACCAAAAAAAATTGCTTGAATATATAGAAGTAAATGGTCCAATTTATATGACAAGTTGTAACTTGTCAAATGCACCTGTATGTAAAACTATTGAAAATGCAAAGCAATTATTCCCCGAAGTTACTAATGTATATAATTTTGGTCCTATGACTCAAAAACCTAGTCAAATAATTAGAGTAGAAGATGGAGAAATTTTAAGAAAATAA", "aa": "MPNQKKLLEYIEVNGPIYMTSCNLSNAPVCKTIENAKQLFPEVTNVYNFGPMTQKPSQIIRVEDGEILRK", "aa_hexdigest": "31e42a5462e277f60c3012de9ffa43e2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "tsaC" ], "truncated": "5-prime", "pseudogene": { "start": 557912, "stop": 558379, "observations": { "mutation": [ 558086 ], "stop": [ 558086 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H5", "query_cov": 1.0, "subject_cov": 0.45161290322580644, "identity": 1.0, "score": 147.0, "evalue": 6.82e-44, "gene_start": 1, "gene_end": 70, "reference_start": 86, "reference_end": 155, "reference_sequence": "MINKYQKLIISTTDTVLGIGGPVSKEVEDLIYEIKGRDRNKKLIILVSSIKQARQFKEWNSQADEFAKKYWPGATTIVVNNQGFRMPNQKKLLEYIEVNGPIYMTSCNLSNAPVCKTIENAKQLFPEVTNVYNFGPMTQKPSQIIRVEDGEILRK" }, "paralog": false, "identity": 0.9935483870967742, "score": 308.0, "evalue": 8.5e-110, "description": "Internal stop codon at 558086. Nonsense mutation around 558086.", "psc": { "uniref90_id": "UniRef90_D1J8H5", "gene": "TsaC", "product": "L-threonylcarbamoyladenylate synthase", "uniref50_id": "UniRef50_A0A077L684", "cog_id": "COG0009", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H5", "UniRef:UniRef50_A0A077L684", "COG:COG0009", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L684", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L684" ], "product": "L-threonylcarbamoyladenylate synthase" } }, "id": "DFOKDJOJIG_989", "locus": "GCA013367855_00983" }, { "type": "cds", "sequence": "contig_1", "start": 558381, "stop": 558776, "strand": "+", "frame": 3, "gene": "rpiB", "product": "Ribose-5-phosphate isomerase", "db_xrefs": [ "COG:COG0698", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J8H6", "UniRef:UniRef90_D1J8H6" ], "nt": "ATGAAAACGACAGTTAAAATAACAAGCGATCACGCAGGATATGACGCAAAAATAAACCTTGCAAAAAGATTGGAAAATGAAGGCTACAATGTAGAATTATTTGGCTCAAAAACAAATAACAATAGTGATTCTTATGCGGATGCTGCTATTGAATTAGTTAATGAAATTCACAAGGATGGCAATCCTGATGAAAATAAAAATGTAAAATATGTTGCATTTTGTGGATCAGGTATTGGTATTGAAATGACTTTAAACAAATTTAAAGGCATAAGAGCAGCAAGAGTTACAAATGAAAACGAATCTCACCTAGCAAAATTGCATAATAATGCTAACGTACTATGTATGGGTGGAAGATTGTTAGAAGGTAATGAAATTGAAAAAATTTTTCATAACTGA", "aa": "MKTTVKITSDHAGYDAKINLAKRLENEGYNVELFGSKTNNNSDSYADAAIELVNEIHKDGNPDENKNVKYVAFCGSGIGIEMTLNKFKGIRAARVTNENESHLAKLHNNANVLCMGGRLLEGNEIEKIFHN", "aa_hexdigest": "904795d05bfcdd10308dac323e4c9276", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8H6", "query_cov": 1.0, "subject_cov": 0.8451612903225807, "identity": 0.985, "score": 263.0, "evalue": 1.18e-88, "valid": true, "gene": "RpiB", "product": "Ribose-5-phosphate isomerase", "uniref50_id": "UniRef50_D1J8H6", "cog_id": "COG0698", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_D1J8H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8H6" ], "product": "Ribose-5-phosphate isomerase" }, "genes": [ "rpiB" ], "id": "DFOKDJOJIG_990", "locus": "GCA013367855_00984" }, { "type": "cds", "sequence": "contig_1", "start": 558880, "stop": 559071, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTCTATTTTTGTTTTTATATATATTTTATTTTTCAATCTTAGAAGTATTAAATCTTCCAGATACGTTTTCTACTTTTGATGAAGTTATTCATGCATTACTATCGGCAGCCAAAATTTCTTTCTTAATCATATTTTGTTCCAAGAACAAACAATATGTTTCAATTTTAACTGTTTCTGTATTGGTATAA", "aa": "MSLFLFLYIFYFSILEVLNLPDTFSTFDEVIHALLSAAKISFLIIFCSKNKQYVSILTVSVLV", "aa_hexdigest": "6c0c4c9537bfc19bcab723c3f5889836", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7227.5594, "isoelectric_point": 5.4644666671752935 }, "id": "DFOKDJOJIG_991", "locus": "GCA013367855_00985" }, { "type": "cds", "sequence": "contig_1", "start": 559905, "stop": 560093, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00036493F6", "UniRef:UniRef90_UPI00036493F6" ], "nt": "ATGGTCATGATTATTATTTCAGCCGTTTTATACAATATTGGTGTTGCAACATTTTTGGCTAAGGCCGCCACTGTTGCAACTGGGTTATCTGCATTGGTTCAAGCCATTACTCTAACATTAACTCAAACTGCACCATATTTTGATTATATTTATATAGCAATGAATTTGCCTTTTGTAATTGCATTTTGA", "aa": "MVMIIISAVLYNIGVATFLAKAATVATGLSALVQAITLTLTQTAPYFDYIYIAMNLPFVIAF", "aa_hexdigest": "2a33e9ec126b43479b05ec61adba0bf4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 559874, "stop": 560243, "observations": { "insertion": [ 559901 ], "mutation": [ 559907, 560114 ], "stop": [ 559882, 559907, 560114 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00036493F6", "query_cov": 1.0, "subject_cov": 0.5081967213114754, "identity": 0.9840000000000001, "score": 102.0, "evalue": 1.16e-26, "gene_start": 1, "gene_end": 62, "reference_start": 51, "reference_end": 112, "reference_sequence": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNIWKKFPKKMVMIIISAVLYNIGVATFLAKAATVATGLSALVQAITLTLTQTAPYFAYIYIAMNLPFVIAFWKKILDYLWY" }, "paralog": false, "identity": 0.9672131147540983, "score": 219.0, "evalue": 2.71e-75, "description": "Internal stop codon at 559882, 559907, 560114. Frameshift due to insertion around 559901. Nonsense mutation around 559907, 560114.", "psc": { "uniref90_id": "UniRef90_UPI00036493F6", "uniref50_id": "UniRef50_UPI00036493F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00036493F6", "UniRef:UniRef50_UPI00036493F6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00036493F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00036493F6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6644.936399999999, "isoelectric_point": 5.586556816101075 }, "id": "DFOKDJOJIG_992", "locus": "GCA013367855_00986" }, { "type": "cds", "sequence": "contig_1", "start": 560112, "stop": 560243, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410BF5B", "UniRef:UniRef90_UPI002410BF5B" ], "nt": "ATGAAACATACAGCTGAGGAACTTGCTGAGATTAAAGAACGTAAACGTTTAAAGAAAGAAACCAAAAAAGAAAAGGGATCAGTCGGCAAAATTGACATAAATCCTTATGGGGTAAATTTCTTTAATATTTGA", "aa": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNI", "aa_hexdigest": "3bbac7b9d353dab09e5c0c372e3c1fba", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 560032, "stop": 560243, "observations": { "deletion": [ 560096 ], "mutation": [ 560114 ], "stop": [ 560114 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410BF5B", "query_cov": 1.0, "subject_cov": 0.6142857142857143, "identity": 1.0, "score": 84.0, "evalue": 1.76e-20, "gene_start": 1, "gene_end": 43, "reference_start": 1, "reference_end": 43, "reference_sequence": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNIWKKFPKKNGHDYYFSRFIQYWCCNILG" }, "paralog": false, "identity": 0.9714285714285714, "score": 141.0, "evalue": 7.96e-46, "description": "Internal stop codon at 560114. Frameshift due to deletion around 560096. Nonsense mutation around 560114.", "psc": { "uniref90_id": "UniRef90_UPI002410BF5B", "uniref50_id": "UniRef50_UPI002410BF5B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410BF5B", "UniRef:UniRef50_UPI002410BF5B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410BF5B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410BF5B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5018.7903000000015, "isoelectric_point": 9.630819129943848 }, "id": "DFOKDJOJIG_993", "locus": "GCA013367855_00987" }, { "type": "cds", "sequence": "contig_1", "start": 560410, "stop": 560679, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCACTGCAATTTATTTATTAGTATTTTCAATGTTGGTAAATAAATTCTATCCAAAATACAAGAAAGTAAAAATTGATATTTACACCACAAAACCAGAACTAGTTGCTGAAAGATTCAAACAAATTGGATATGTTCACTCATTTAATGTATTCAACGGATTTGGCGGATATAACCATCAAGATTTTGGAAAAATTGAAACAATTGCCCTATATTTAGAAAAAGAATCAATTTTAGAAGAAGTTAGAAGCGTTGATAAAAAAGCCTGA", "aa": "MGTAIYLLVFSMLVNKFYPKYKKVKIDIYTTKPELVAERFKQIGYVHSFNVFNGFGGYNHQDFGKIETIALYLEKESILEEVRSVDKKA", "aa_hexdigest": "a94a705ec2306d1b508f616e5dc9198e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10361.939599999994, "isoelectric_point": 9.098632621765137 }, "id": "DFOKDJOJIG_994", "locus": "GCA013367855_00988" }, { "type": "cds", "sequence": "contig_1", "start": 560941, "stop": 561147, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCCAGAGATGATTTAGCTATTGCTAAGTATCTAGCCAACTTATTTAAAACTAATGATTTAAAAATGGTATTTGATAATGCAAAATTAATTTTTGAAAAATCTAATTCTTTATCACTTGCTAATATCCCTAGTCTATTAACAGGTATTGATTTAGTAGGTGGTAAAAATACTAAAATTTATGGAATATATAATGGCCAAAATTGA", "aa": "MSRDDLAIAKYLANLFKTNDLKMVFDNAKLIFEKSNSLSLANIPSLLTGIDLVGGKNTKIYGIYNGQN", "aa_hexdigest": "eb8fd00734eae66fc9732435bb3084e1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7495.584099999997, "isoelectric_point": 9.049507713317869 }, "id": "DFOKDJOJIG_995", "locus": "GCA013367855_00989" }, { "type": "cds", "sequence": "contig_1", "start": 561457, "stop": 561636, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGTAAGTGCATTTTTATATAATATTGCAATTACAGTATTTTTAAAAAAAGCAGCAACAATAGCTTCAGGAACTTCATCTTTATCTCAAATAATTACTTTTACAGCGCCTGCAACTGCACAATTTTATGGTTTATTTTATGTATTAGTTAACCTACCTTTAATGTTTATTTATTGA", "aa": "MLVSAFLYNIAITVFLKKAATIASGTSSLSQIITFTAPATAQFYGLFYVLVNLPLMFIY", "aa_hexdigest": "1bf202222e35b1ddb3f6d176f53dd0d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6456.647599999998, "isoelectric_point": 9.303577613830566 }, "id": "DFOKDJOJIG_996", "locus": "GCA013367855_00990" }, { "type": "cds", "sequence": "contig_1", "start": 561664, "stop": 561792, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTAGAAGATAAAAAAATAACTAAAATCAATAAAATCATTCTTACTAAAGAGCAAAAACACCAACGGTATTTAGAAAAAATGAAAATTCTAAATCCACAAAAAATAAATTTAATAAATGTCTGA", "aa": "MSLEDKKITKINKIILTKEQKHQRYLEKMKILNPQKINLINV", "aa_hexdigest": "1873dbb81ad7f096745ecd14bcf5105a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5077.1061, "isoelectric_point": 9.998482322692869 }, "id": "DFOKDJOJIG_997", "locus": "GCA013367855_00991" }, { "type": "cds", "sequence": "contig_1", "start": 561832, "stop": 562365, "strand": "-", "frame": 1, "gene": null, "product": "Replicative DNA helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A6S1" ], "nt": "ATGTATTTAAATGATAAACCTACATCAACAATTTCGGATATAACAACCATTGCAAAACGATTTGCTAGAAACAATAAAGTTGATCTAGTTGTAGTTGATTATCTACAATTAATGTCAGATTCTTCAAAAGCAAGTATGGAAAATCGTCAATTGGAAGTAAGCAAGATTTCGAGATCCTTAAAGCAACTTGCTAGAGAATTAGAATGTCCTGTTTTAGCATTAAGCCAGTTAAGTAGAAATGTTGAAAAAAGGGAAGATAAAACTCCTATTTTGTCAGATTTAAGAGAATCAGGAACAATTGAACAAGATGCCGATATGGTTATTTTTTTACATAGAAAAGATTATTATAATAAGCACAACATTAATGATGACAATGATAATGCAGAAGCTGTTGACAAAAGTGAAATTGTCAATGGCAGTTCTTCTTTAACCAATGTCATTGTAGCAAAAAATAGACATGGGGCAATTGGAATTGTACAACTATTATTTCAACCAGAATGCAACAGGTTTATTTATGAAAAAAAGAAAAATTAA", "aa": "MYLNDKPTSTISDITTIAKRFARNNKVDLVVVDYLQLMSDSSKASMENRQLEVSKISRSLKQLARELECPVLALSQLSRNVEKREDKTPILSDLRESGTIEQDADMVIFLHRKDYYNKHNINDDNDNAEAVDKSEIVNGSSSLTNVIVAKNRHGAIGIVQLLFQPECNRFIYEKKKN", "aa_hexdigest": "a93d8a490f06edc970265a62e972f4e8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P211", "query_cov": 0.9322033898305084, "subject_cov": 0.9116022099447514, "identity": 0.703, "score": 218.0, "evalue": 1.17e-69, "valid": false, "product": "Replicative DNA helicase", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_A0A449A6S1", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_A0A449A6S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A6S1" ], "product": "Replicative DNA helicase" }, "genes": [], "id": "DFOKDJOJIG_998", "locus": "GCA013367855_00992" }, { "type": "cds", "sequence": "contig_1", "start": 562393, "stop": 562836, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03796.21" ], "nt": "ATGGAAAACAAGCCTTATGAAATTTCAGATTTATTATCCAATTTACAATTGAATTTAATCAACCTAGATATTTCAGAAATAAATACTGCTTATGAACAAGTAGGAAATAAAGCAGATGAACTATTTAATAGAATAATGAGCAAAGAAAAATCAGATATTGGCGTTGGCCTTTCTTTTGGATTTCCACCATTGGATGAATTAATTTTAGGCGCCAATCCCGGCGATTTGATAATTTTGGCAGCTCGCCCAGCAATGGGAAAAACTGCTTTTGCTTTAAATATAGCAAATAATGTTGCTAAAAACAATAAAACGGTGCTATTTTTTTCTCTTGAAATGTCAAATTCACAAATTGTTCAAAGAATGATGTCAATTGATTCCTTGGTTCCGATTAGTTTAATGAGAAAAAATGAAATCTCAACAGAAAACGCTAAAAATCTAGCCTGA", "aa": "MENKPYEISDLLSNLQLNLINLDISEINTAYEQVGNKADELFNRIMSKEKSDIGVGLSFGFPPLDELILGANPGDLIILAARPAMGKTAFALNIANNVAKNNKTVLFFSLEMSNSQIVQRMMSIDSLVPISLMRKNEISTENAKNLA", "aa_hexdigest": "5c5f2f98c12d853157e1609e02d6be83", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03796.21", "name": "DnaB_C", "length": 92, "aa_cov": 0.6190476190476191, "hmm_cov": 1.0, "evalue": 8.971765921076416e-30, "score": 99.5001449584961, "start": 56, "stop": 146 } ], "seq_stats": { "molecular_weight": 16173.475199999992, "isoelectric_point": 4.827301597595214 }, "id": "DFOKDJOJIG_999", "locus": "GCA013367855_00993" }, { "type": "cds", "sequence": "contig_1", "start": 562948, "stop": 563229, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00772.26" ], "nt": "ATGAACATTCAAGAAGAAAGAGAATTAGCAATTATTAATAATGAAACATCGTTATTAGGTTTATTAATTAGGGAACCAAATTGTTATTTAACAATTGCTGAAATTTTAAAACCTGAAATGTTTCATTTTAAAAATAATCAAATTCTTTTTAAATCTATTTCAGAAGTTCATATTGCCTCTAAACAATTCGATATAGCCAACTTATTGAGTTATTTAAATGACCAAAAATTAATTGATCACATTGGTTCGTTTGGCTATAATGGTAGTGAATATATTAGCTGA", "aa": "MNIQEERELAIINNETSLLGLLIREPNCYLTIAEILKPEMFHFKNNQILFKSISEVHIASKQFDIANLLSYLNDQKLIDHIGSFGYNGSEYIS", "aa_hexdigest": "de7f77af7d54a33655db8caec9ccc4c4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00772.26", "name": "DnaB", "length": 75, "aa_cov": 0.8064516129032258, "hmm_cov": 1.0, "evalue": 1.0083131036980883e-15, "score": 53.34480285644531, "start": 12, "stop": 86 } ], "seq_stats": { "molecular_weight": 10717.135599999989, "isoelectric_point": 5.0043550491333 }, "id": "DFOKDJOJIG_1000", "locus": "GCA013367855_00994" }, { "type": "cds", "sequence": "contig_1", "start": 563256, "stop": 563696, "strand": "-", "frame": 2, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855666.1", "SO:0001217", "UniParc:UPI0001BC23CE", "UniRef:UniRef100_D1J8I0", "UniRef:UniRef50_A0A318U7T8", "UniRef:UniRef90_D1J8I0" ], "nt": "ATGAAAGTAATATTAATTAAAGAATACCAAAAACATAAAAAAAATGAAATCATTGAAGTTACAGACGGTTTTGCAAAAAACTTTTTAATTAAAAATGGTTATGCTCAACCAATTAACAAACAAACATTGGCAAATTTAGATAGAGTTAAATCAAATATTGCAGAAGATTTAGCAAATCAAATAGCCAAGGCTAATGAAATCAAACAATTAATTGAATCATTAGTTTTAAAATTCTATTTAAAATCTAATGGAAATATAGTGCACGGAAACGTTACAACAAAAGCAATTGAAAAAGAATTAATAAAAAACAACATTAAAATCCCAAGCCATGCATTTGAATCAGGGGTAACATATAATACTTTTGGGTTGCATGATGTAAAAATTAAATTACATGATCAAGTAACTGCAACACTAAAAATAAATATCATAGAAGAGAAATAA", "aa": "MKVILIKEYQKHKKNEIIEVTDGFAKNFLIKNGYAQPINKQTLANLDRVKSNIAEDLANQIAKANEIKQLIESLVLKFYLKSNGNIVHGNVTTKAIEKELIKNNIKIPSHAFESGVTYNTFGLHDVKIKLHDQVTATLKINIIEEK", "aa_hexdigest": "ec4e630ac277d7d311d0830ac1393afd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC23CE", "ncbi_nrp_id": "WP_012855666.1", "uniref100_id": "UniRef100_D1J8I0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC23CE", "RefSeq:WP_012855666.1", "UniRef:UniRef100_D1J8I0" ] }, "ips": { "uniref100_id": "UniRef100_D1J8I0", "uniref90_id": "UniRef90_D1J8I0" }, "psc": { "uniref90_id": "UniRef90_D1J8I0", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_A0A318U7T8", "cog_id": "COG0359", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318U7T8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318U7T8" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "DFOKDJOJIG_1001", "locus": "GCA013367855_00995" }, { "type": "cds", "sequence": "contig_1", "start": 563677, "stop": 564000, "strand": "-", "frame": 1, "gene": null, "product": "DHHA1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9M3" ], "nt": "TTGCAAGAAATTAAAAAAGGATATTTCTTGGCATACAAAGATATTGAAGCATCAACAGATATTATTTCAATAGCGTCAGATGAAATATTAAGAGTTAATGGTAGGAAAGCTGCATTTGTTGTTGCTAAATTACCAGGAACAAAAACATACAAAATGTCAGCAAGAGGAATTAATACAAATGTTCAAATTATAGCAGAAGCAGTTAACGGCGGTGGCCATTTTGGGGCAGCCGCTGCTGAAACAACTGAAAATTTGAATTTATTTGTTGATAATATAATACAAGCAATAGTGAGTGTGAAAGATAATGAAAGTAATATTAATTAA", "aa": "MQEIKKGYFLAYKDIEASTDIISIASDEILRVNGRKAAFVVAKLPGTKTYKMSARGINTNVQIIAEAVNGGGHFGAAAAETTENLNLFVDNIIQAIVSVKDNESNIN", "aa_hexdigest": "203427b7f42c4d179281510b7caf3d55", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P200", "query_cov": 1.0, "subject_cov": 1.009433962264151, "identity": 0.757, "score": 152.0, "evalue": 7.27e-46, "valid": false, "product": "Putative bifunctional signaling protein/50S ribosomal protein L9", "uniref50_id": "UniRef50_A0A432F9M3" }, "pscc": { "uniref50_id": "UniRef50_A0A432F9M3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9M3" ], "product": "DHHA1 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_1002", "locus": "GCA013367855_00996" }, { "type": "cds", "sequence": "contig_1", "start": 564082, "stop": 565245, "strand": "-", "frame": 1, "gene": "gdpP", "product": "GGDEF domain-containing protein", "db_xrefs": [ "COG:COG3887", "COG:T", "EC:3.1.4.59", "KEGG:K22927", "SO:0001217", "UniRef:UniRef50_A0A448ZXC6", "UniRef:UniRef90_D1J8I1" ], "nt": "ATGTATGAAATAGACGGATGCAATTACGAAGTTCATATATCATTTGAAAAAAATCTAGTTATTTTAAGAGATGTTACTGCTCAACAAAATTTATTAGATGACTATAAAAAACAAAGAATTGTGTTTGGGGAATTGGACATTGACAATATTAATTTATATCAAACATTTTTATCCCAAGAAGAAATTTTTAAAATTTATGCTTCTGTAGTAAATTTATTAGATGAAATATCTAAAAAATACAATGTTATTTATAGACAATACGAAAACGGAAGATTCTTTTTAATAACAAATAAAGAAACATTAGAAGAATTAGAACGAGATAGTTTTAATGTTATAACTTCAGTAAAAAACAAAATAATTGTTAAAGATGTTTTGATATCAATTTCTGGTGGATTTGGTTATGGTATTTTTAAATACGATACGTTAGTCAAATTAGCAAAAGATGCTCTAATGCAATCTCAAATGCGGGGTGGCGATCAAATTACAGTTTTAACAAAAAATGAAAAAGCTAGACACTATGGCTCAAGCTCGGAAATTGATGTAAATCTATCAAGGACAAATGTAAACTATATAGCACAAAACTTATTAGAAAAGATAAAATCAAAAGATATTGAAAATGTTATAGCCTATGGCCATAAGAATGCAGACCTTGATGCTTTGGGTTCAACATATGCAATTTTGCAATTAGCATTAGCCTATAAAAAGAATGCTTATATTCAAAACAAGACATTTGATAGTACTACTCAAAGAGTTATTGATAAAATGTCGAAGGAAACTCAAGAATATTTTATTAGTCCTAAAGAAGCAGAAAGCATCAACAATGAAAAAACATTGGTTGTAATTTGCGATACTTCAGATGATACTAGAATTGAAAATGCTAATGTCTTTAAAAACATAAAAAAAGAAAATATCGTGATAATTGATCATCATAGAATAAATAAAAATACTGATGAAATAGCATATAGAGAAAATGTATATATTGATTCCTCGGCGTCATCTGCTTCTGAAATATTAACAGAAATCATCACATTGTTACACGAAGAAGATAAAATTAATAATGATACAGCGCAATATTTATTAGATGGAATTTATTTAGATACTAATACATTTAAAAAGCAAACAAGTGCTAAAACTTTTGCAGCAGCATCAATATTGCAAAAATGA", "aa": "MYEIDGCNYEVHISFEKNLVILRDVTAQQNLLDDYKKQRIVFGELDIDNINLYQTFLSQEEIFKIYASVVNLLDEISKKYNVIYRQYENGRFFLITNKETLEELERDSFNVITSVKNKIIVKDVLISISGGFGYGIFKYDTLVKLAKDALMQSQMRGGDQITVLTKNEKARHYGSSSEIDVNLSRTNVNYIAQNLLEKIKSKDIENVIAYGHKNADLDALGSTYAILQLALAYKKNAYIQNKTFDSTTQRVIDKMSKETQEYFISPKEAESINNEKTLVVICDTSDDTRIENANVFKNIKKENIVIIDHHRINKNTDEIAYRENVYIDSSASSASEILTEIITLLHEEDKINNDTAQYLLDGIYLDTNTFKKQTSAKTFAAASILQK", "aa_hexdigest": "8359095409da7b4c571944f5c7cceb51", "start_type": "ATG", "rbs_motif": null, "genes": [ "gdpP" ], "truncated": "both", "pseudogene": { "start": 563779, "stop": 565545, "observations": { "mutation": [ 564084, 565269, 565350 ], "stop": [ 564084, 565269, 565350 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I1", "query_cov": 1.0, "subject_cov": 0.589041095890411, "identity": 0.992, "score": 740.0, "evalue": 3.35e-265, "gene_start": 1, "gene_end": 387, "reference_start": 136, "reference_end": 522, "reference_sequence": "MKNKDIQKIYIALQVILLFILPTILFFIGIFCLKTQQSKIALMAIYTLFLIGLAIYLTIILQRYFKNSILAKKSLEFYLQSEFNQFGTGILIFLNSGKIIWASESLKIKFHNNLINKNIKDIFNIKQWTSDNQEFMYEIDGCNYEVHISFEKNLVILRDVTAQQNLLDDYKKQRIVFGELDIDNINLYQTFLSQEEIFKIYASVVNLLDEISKKYNVIYRQYENGRFFLITNKETLEELERDSFNVITSIKNKIIVKDVLISISGGFGYGIFKYDTLVKLAKDALMQSQMRGGDQITVLTKNEKARHYGSSSEIDVNLSRTNVNYIAQNLLEKIKSKDIENVIAYGHKNADLDALGSTYAILQLALAYKKNAYIQNKTFDSTTQRVIDKMSKETQEYFISPKEAESINNEKTLVVICDTSDDTRIENANAFKNIKKENIMIIDHHRINKNTDEIAYRENVYIDSSASSASEILTEIITLLHEEDKINNDTAQYLLDGIYLDTNTFKKQTSAKTFAAASILQKWGAQNDKSVDTLKFDEKVFKIVQELLANLQEIKKGYFLAYKDIEASTDIISIASDEILRVNGRKAAFVVAKLPGTKTYKMSARGINTNVQIIAEAVNGGGHFGAAAAETTENLNLFVDNIIQAIVSVKDNESNIN" }, "paralog": false, "identity": 0.9897959183673469, "score": 1122.0, "evalue": 0.0, "description": "Internal stop codon at 564084, 565269, 565350. Nonsense mutation around 564084, 565269, 565350.", "psc": { "uniref90_id": "UniRef90_D1J8I1", "gene": "GdpP", "product": "GGDEF domain-containing protein", "ec_ids": [ "3.1.4.59" ], "uniref50_id": "UniRef50_A0A448ZXC6", "cog_id": "COG3887", "cog_category": "T", "kegg_orthology_id": "K22927", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I1", "EC:3.1.4.59", "UniRef:UniRef50_A0A448ZXC6", "COG:COG3887", "COG:T", "KEGG:K22927" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXC6" ], "product": "Probable manganese-dependent inorganic pyrophosphatase" } }, "id": "DFOKDJOJIG_1003", "locus": "GCA013367855_00997" }, { "type": "cds", "sequence": "contig_1", "start": 565348, "stop": 565650, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACAAAGATATTCAAAAAATTTATATAGCTTTACAAGTTATCCTATTATTTATTTTGCCAACAATATTATTCTTTATTGGCATTTTTTGTTTAAAGACTCAACAGTCAAAAATAGCGTTAATGGCAATTTACACTTTATTTTTAATCGGCCTTGCAATTTATTTAACTATTATTTTGCAACGTTATTTTAAAAACTCAATATTAGCTAAAAAATCATTGGAATTTTATTTACAAAGTGAGTTTAATCAATTTGGTACGGGAATTTTGATATTTTTAAATAGTGGAAAAATTATATGA", "aa": "MKNKDIQKIYIALQVILLFILPTILFFIGIFCLKTQQSKIALMAIYTLFLIGLAIYLTIILQRYFKNSILAKKSLEFYLQSEFNQFGTGILIFLNSGKII", "aa_hexdigest": "93913c5e64e6e5d77a593477eabc9f08", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11558.992599999985, "isoelectric_point": 9.763881874084472 }, "id": "DFOKDJOJIG_1004", "locus": "GCA013367855_00998" }, { "type": "cds", "sequence": "contig_1", "start": 565704, "stop": 566009, "strand": "-", "frame": 2, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "SO:0001217", "UniRef:UniRef50_B3PN57", "UniRef:UniRef90_D1J8I2" ], "nt": "TTGCAAGATAAAATTTCTTTTATGATTGACTTTTATTTTAAAAATGATAAAAACAATAAAAAAGTGTTGCTATTAATTGATGCTAAAATTGGTTTTAGCGCTAGTGATTTAGAAATAATAGAATATCTTAACGATCTTAAAATAAAATTTGATATAGTTGTTACAAAAATCGACAAGGCAAATCAATCTCAACGTTATTGCGTTAAAAATCAAGCCTTGTCTTTAAAAACAGCTGAAAATATTTTTTTGGTATCATCTGAAAAAAAACAAGGTATTGAAGAAATAATGCAATTTTATGACTTGTAG", "aa": "MQDKISFMIDFYFKNDKNNKKVLLLIDAKIGFSASDLEIIEYLNDLKIKFDIVVTKIDKANQSQRYCVKNQALSLKTAENIFLVSSEKKQGIEEIMQFYDL", "aa_hexdigest": "25993391771d48c21cb740aee31b661a", "start_type": "TTG", "rbs_motif": null, "genes": [ "yihA" ], "truncated": "3-prime", "pseudogene": { "start": 565704, "stop": 566255, "observations": { "mutation": [ 566183, 566252 ], "stop": [ 566183, 566252 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I2", "query_cov": 1.0, "subject_cov": 0.5519125683060109, "identity": 0.98, "score": 195.0, "evalue": 5.7e-62, "gene_start": 1, "gene_end": 101, "reference_start": 83, "reference_end": 183, "reference_sequence": "MWKFLKSCKSHDDFINSDNIQYVFWGRSNVGKSSLINSLASCNIAKTSSYPGRTQLINYFESNKNKIIIDLPGYGYAKISKKLQDKISFMIDFYFKNDKNNKKVLLLIDAKIGFSASDLEIIEYLNDLKIKFDIVVTKIDKANQSQRYCVKNQALSLKTVENIFLVSSEKKQGIEEIMQFYDL" }, "paralog": false, "identity": 0.9836065573770492, "score": 353.0, "evalue": 1.14e-126, "description": "Internal stop codon at 566183, 566252. Nonsense mutation around 566183, 566252.", "psc": { "uniref90_id": "UniRef90_D1J8I2", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_B3PN57", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I2", "UniRef:UniRef50_B3PN57", "COG:COG0218", "COG:D", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN57" ], "product": "Probable GTP-binding protein EngB" } }, "id": "DFOKDJOJIG_1005", "locus": "GCA013367855_00999" }, { "type": "cds", "sequence": "contig_1", "start": 566255, "stop": 566641, "strand": "-", "frame": 3, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q6KIG0", "UniRef:UniRef90_D1J8I3" ], "nt": "ATGGAATTTAGAAATAAATACACAGAACATTTTACTATTCCACATACTGAAGCTCAATTGAACTTTTCATTTAGTGGATTAAAAACACAAGTTATAAATCTTATAAATAACCAAATCAATAGAAAACAAGAGGTCAATGTTGATCAATATGCAACAATGTTTGAAAATACAGTAGTTTTATATTTAAAAAATCATTTAGAAATTGCAATTAAAAAATATCATCCAAGAGTAATAGTTTTGGCAGGCGGAGTAAGTGCTAATAAAGCAATTAGAGAAATGTTTTTAAATGTCCATTCAAATGCCATAATTCCGGAAATGAAATATACAACTGATAATGCAATGATGATTGCTAGGTTAGCATATGAACTATTAAGGAGAGATAGATAA", "aa": "MEFRNKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEVNVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR", "aa_hexdigest": "4079d60220b64a4b20670d7f332b1fbc", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 566255, "stop": 566941, "observations": { "mutation": [ 566644 ], "stop": [ 566644 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I3", "query_cov": 1.0, "subject_cov": 0.4115755627009646, "identity": 0.9840000000000001, "score": 249.0, "evalue": 5.36e-81, "gene_start": 1, "gene_end": 128, "reference_start": 184, "reference_end": 311, "reference_sequence": "MLIFAIESSHDDTSFALLEDNKPIWMETISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKKWMEFRDKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR" }, "paralog": false, "identity": 0.9868421052631579, "score": 448.0, "evalue": 7.99e-162, "description": "Internal stop codon at 566644. Nonsense mutation around 566644.", "psc": { "uniref90_id": "UniRef90_D1J8I3", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q6KIG0", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I3", "EC:2.3.1.234", "UniRef:UniRef50_Q6KIG0", "COG:COG0533", "COG:J", "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG0" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "DFOKDJOJIG_1006", "locus": "GCA013367855_01000" }, { "type": "cds", "sequence": "contig_1", "start": 566642, "stop": 567115, "strand": "-", "frame": 3, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q6KIG0", "UniRef:UniRef90_D1J8I3" ], "nt": "ATGGAAACAATTAGCCAAATAGACATTCATAGAAAATATGGAGGCACAGTTCCAGAATTAGCATCAAGATTGCATGTAAAAAATATAGGTATTTTAATTGAAAAATTAAAAAAAATAATTGATATTAAAAAGATTGACTATGTAGCCTATACAAAAGAACCAGGATTAGTTGGTTCTTTACATGTTGGATATGTTGTTGCCAATGCAATAGCAATGTTACTTGAAAAACCTATATTGCCTTTGAATCATTTAGAAGGGCATTTTTTCTCTGCTTTTATAGGCAAAGAAGTTGAATACCCTGCTTTAGGTTTAATTGTTTCAGGTGGGCATTCACAATTAATGTTATATAAATCAAAAAATGAATACGAAGTTATAGGTCAAACTCAAGATGATGCCGTCGGAGAAGTATTTGATAAAATAGCAAGAAAATTAAATTTGGGTTTTCCTGGAGGACCTATTATTGATAAAAAATGA", "aa": "METISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKK", "aa_hexdigest": "2d6f9daa68d37cb12cda0f81d33e3a2a", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "both", "pseudogene": { "start": 566339, "stop": 567190, "observations": { "mutation": [ 566644, 567118 ], "stop": [ 566644, 567118 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I3", "query_cov": 1.0, "subject_cov": 0.5048231511254019, "identity": 1.0, "score": 315.0, "evalue": 1.89e-106, "gene_start": 1, "gene_end": 157, "reference_start": 26, "reference_end": 182, "reference_sequence": "MLIFAIESSHDDTSFALLEDNKPIWMETISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKKWMEFRDKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR" }, "paralog": false, "identity": 0.9858657243816255, "score": 551.0, "evalue": 2.79e-202, "description": "Internal stop codon at 566644, 567118. Nonsense mutation around 566644, 567118.", "psc": { "uniref90_id": "UniRef90_D1J8I3", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q6KIG0", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I3", "EC:2.3.1.234", "UniRef:UniRef50_Q6KIG0", "COG:COG0533", "COG:J", "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG0" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "DFOKDJOJIG_1007", "locus": "GCA013367855_01001" }, { "type": "cds", "sequence": "contig_1", "start": 567184, "stop": 567738, "strand": "-", "frame": 1, "gene": "tsaB", "product": "Gcp-like domain-containing protein", "db_xrefs": [ "COG:COG1214", "COG:J", "RefSeq:WP_036432569.1", "SO:0001217", "UniParc:UPI0005142A2E", "UniRef:UniRef100_UPI0005142A2E", "UniRef:UniRef50_D1J8I4", "UniRef:UniRef90_D1J8I4" ], "nt": "GTGAAATTATTCATTGAAACTTCATTAAGCGATTTTTATATAAGCTTAATTGATGAAAATTATTGCGAGATTGATTATATTTTTATTGCTAACTTAGTTAAAAAAACCGATGCATTTTTTGAAAATTTAAATTATTTATTAAATAAAAATAATTTTAAATTAAGTTCAATTTCAGAAATTTATACAACGTTAGGACCTGGGAGTTTTTCAGGTGCAAGAATTGGCTTAGTTTTTGCCAAAACAGTAGCACAATTATCAAATATCAAATTATTTGTAGCCCCAACATATTCTTTATTTTATACACAAAAAAAATTGCAACATGAAGATTGCAATTTAATAAATATAAAAGCCAATAAATACAATGTTTATTCAATAATTGTTGATCCATTTGAATGTCATATGCAAGAAAACTTAGGCAATTTTGATTTATTCAATAAAGAGCTTTTTTCAAAAAATATAGAGCAATATTTAAAAGAATTTAAACAAGTAAATGATATTGAAAATTGTGAATTAATTTATTTGCATGATCCACAAATAGGAGAGATAAAATGTTAA", "aa": "MKLFIETSLSDFYISLIDENYCEIDYIFIANLVKKTDAFFENLNYLLNKNNFKLSSISEIYTTLGPGSFSGARIGLVFAKTVAQLSNIKLFVAPTYSLFYTQKKLQHEDCNLINIKANKYNVYSIIVDPFECHMQENLGNFDLFNKELFSKNIEQYLKEFKQVNDIENCELIYLHDPQIGEIKC", "aa_hexdigest": "907cedefd291b28e72d914fba57be651", "start_type": "GTG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0005142A2E", "ncbi_nrp_id": "WP_036432569.1", "uniref100_id": "UniRef100_UPI0005142A2E", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005142A2E", "RefSeq:WP_036432569.1", "UniRef:UniRef100_UPI0005142A2E" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005142A2E", "uniref90_id": "UniRef90_D1J8I4" }, "psc": { "uniref90_id": "UniRef90_D1J8I4", "gene": "TsaB", "product": "Gcp-like domain-containing protein", "uniref50_id": "UniRef50_D1J8I4", "cog_id": "COG1214", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J8I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8I4" ], "product": "Gcp-like domain-containing protein" }, "genes": [ "tsaB" ], "id": "DFOKDJOJIG_1008", "locus": "GCA013367855_01002" }, { "type": "cds", "sequence": "contig_1", "start": 567840, "stop": 568136, "strand": "-", "frame": 2, "gene": "tsaE", "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE", "db_xrefs": [ "COG:COG0802", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A3G8LG14", "UniRef:UniRef90_D1J8I5" ], "nt": "ATGTCAAATCTAAAATTTATTTATAAAAATAACGAACCATTAGATAAACTAGTTGACTATATTTTGTCATTTAAAAATTTAGAAGCCTTGCTTTTAATAGGCGAACTCGGAGCAGGCAAAACAACTTTAACAAGTCAAATAGCAAAAAAATTAAACGAACCAAAGACTGTAATATCACCAACATTCAATACAATTTTAGTGTATGATAAATTTGTACATATTGATGCTTATAAATTAACTACAAATTTATTTGCTTATGAAGATTATTTTGAAGATAAATTAGCAATTATTGAATGA", "aa": "MSNLKFIYKNNEPLDKLVDYILSFKNLEALLLIGELGAGKTTLTSQIAKKLNEPKTVISPTFNTILVYDKFVHIDAYKLTTNLFAYEDYFEDKLAIIE", "aa_hexdigest": "d4dfdacce5f9d287a456a83f5ae9929e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "tsaE" ], "truncated": "5-prime", "pseudogene": { "start": 567738, "stop": 568142, "observations": { "mutation": [ 567842 ], "stop": [ 567842 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I5", "query_cov": 1.0, "subject_cov": 0.7313432835820896, "identity": 1.0, "score": 191.0, "evalue": 3.45e-61, "gene_start": 1, "gene_end": 98, "reference_start": 3, "reference_end": 100, "reference_sequence": "EIMSNLKFIYKNNEPLDKLVDYILSFKNLEALLLIGELGAGKTTLTSQIAKKLNEPKTVISPTFNTILVYDKFVHIDAYKLTTNLFAYEDYFEDKLAIIEWAENVTLPKFKHYIKIKVYFDNEQNHVFEVMEEQ" }, "paralog": false, "identity": 0.9850746268656716, "score": 264.0, "evalue": 1.74e-92, "description": "Internal stop codon at 567842. Nonsense mutation around 567842.", "psc": { "uniref90_id": "UniRef90_D1J8I5", "gene": "TsaE", "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE", "uniref50_id": "UniRef50_A0A3G8LG14", "cog_id": "COG0802", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I5", "UniRef:UniRef50_A0A3G8LG14", "COG:COG0802", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LG14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LG14" ], "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE" } }, "id": "DFOKDJOJIG_1009", "locus": "GCA013367855_01003" }, { "type": "cds", "sequence": "contig_1", "start": 568320, "stop": 569009, "strand": "-", "frame": 2, "gene": "ysdC", "product": "Aminopeptidase ysdC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "nt": "ATGCAAGAAGAAGAAAGAAAGAAAGCAATTACAGAAAATGAATTATTTGCTGATTTTGGTTTCAAAAGCGATGAAGATGCTATTGCTCATGGTGTTAATGTTGGCGATAGAGTTTACATGAGTGGAGAAACAATACTATTTAATGATCAAAATTTAATTGGTGGAAAAGCAATGGACAACCGTGCTGGTGTAACCGTGTTAGAATTTGTTGCTAAAGCTGTTGCAAAATTAGATCTTGACATTGATCTATATTTAGTAGGTACAGTTCAAGAAGAAGTTGGAACAAGAGGAGCTAAAACAGTTGCGTCTATGATTGATCCAGACATTGCGATAGCAGTTGATACTTCTGCATCTCACGATACACCTAATACAATTCCTGGAAATGTTTCTTTATATAAAGGAGCTGCTTTAAGATTAAAAGATAGAGGAACAATGATGGATCCAAAATTGGTACAATTTTTTGTTGATTTATCTAAGAAATATAATATTCCAACATTTAAATATGTAGCCATGGGCGGGGGTACTGATGCCTTTGAATTGCAATTTTCAAAAGGCGGAGCAGCCACATTAACAATTTCATTACCTCAAAGATATTTGCATTCACCAATCGGAGTATGTTGCATAAGTGATTTATTGGCAGCCGTTGATTTGTTAACAAAATTTTTAACTGATTTTAAACAATCTGATTGA", "aa": "MQEEERKKAITENELFADFGFKSDEDAIAHGVNVGDRVYMSGETILFNDQNLIGGKAMDNRAGVTVLEFVAKAVAKLDLDIDLYLVGTVQEEVGTRGAKTVASMIDPDIAIAVDTSASHDTPNTIPGNVSLYKGAALRLKDRGTMMDPKLVQFFVDLSKKYNIPTFKYVAMGGGTDAFELQFSKGGAATLTISLPQRYLHSPIGVCCISDLLAAVDLLTKFLTDFKQSD", "aa_hexdigest": "2650e29473978c1d547374998b132b41", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE1AB9A", "query_cov": 0.8733624454148472, "subject_cov": 0.9478672985781991, "identity": 0.735, "score": 296.0, "evalue": 1.58e-99, "valid": false, "product": "M42 glutamyl aminopeptidase", "uniref50_id": "UniRef50_A0A3B0P1S5" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P1S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "product": "Aminopeptidase ysdC" }, "genes": [ "ysdC" ], "id": "DFOKDJOJIG_1010", "locus": "GCA013367855_01004" }, { "type": "cds", "sequence": "contig_1", "start": 569100, "stop": 569384, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGAGAAAATCAATTCAAAGAAAGATTAATAAAATATATGGAAATTGAAGCAATGTCTCGTTATGAAGAACCAGTTGTTAATGAATTAAAAAAAGAATTAGCTAATGAAGGATTTAAATTTAGCAGAGATAATTTTGGCTGTTTAATTATGCACAAGCCATCTAAAAATCCAAATGCTCCTAAAGTTTTATTAGCAGCACACATGGATGAAGTTGGATATGTAGTAAGAAATTTTGATAAAAAAGGACAATTATATTTATCAACAGTTGGTGGTGTATGA", "aa": "MTRENQFKERLIKYMEIEAMSRYEEPVVNELKKELANEGFKFSRDNFGCLIMHKPSKNPNAPKVLLAAHMDEVGYVVRNFDKKGQLYLSTVGGV", "aa_hexdigest": "ac5a9faf455d4a3e4ed24d6846768d09", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10868.465599999992, "isoelectric_point": 8.709501457214355 }, "id": "DFOKDJOJIG_1011", "locus": "GCA013367855_01005" }, { "type": "rRNA", "sequence": "contig_1", "start": 569514, "stop": 572409, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9900854700854701, "score": 2767.2, "evalue": 5e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TATTAAACAAGCAAGAGTTTTTGGTGGATGCCTTGGGTCTGGAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGGTTAGCTGGAAATAAGCTGTTATCCGGGGATTTCCGAATGGGGAAACCTAATTGAGCTAATCCTCAATTATCATATCGATGAATTCATAGTCGAATGAAGAGACACGCTGTGAATTGAAACATCTCAGTAGCAGCAGGAAGAGAAAATAAAGAATGATTCCCTAAGTAGTGGCGAGCGAACGGGGAAGAGCCCAAACCAACACATGTTGTTGGGGTTGTAGGACTGCGACATGGATTAATGAATTTGTACATAGCAGAATAAGTTGGAACGCTTAAACATAGAGGGTGAAATTCCCGTAAGCGAAATGTATAAATCTCCTAGCAGTATCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATTTGCCGGGACCACCCGGTAAGGCTAAATACTAACCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGGGAGTGAAATAGATTCTGAAACCAATTACTTACAGTTAGTCAGAGCCCGTTAATGGGTGATGGCGTACATCTTGCAGAATGGACCGGCGAGTTATGTCAACATGCGAGGTTAAGTAGAATAAAGCGAAGCCGTAGAGAAATCGAGTCTGAATAGGGCGATTTAGTATGTTGATATAGACCCGAAACCAGGTGATCTACCCATGAGCAGGTTGAAACTTAGGTAACACTAAGTGGAGGACCGAACCGCAGTACGCTAAAAAGTGCCCGGATGACTTGTGGGTAGGGGTGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAATGATGGGGGTAGAGCACTGAATATGGAATGGCGGCGCCTAGCCGTACTGACTATAATCAAACTCCGAATACTATCATGTATTACTATGCAGTCGGTACATCGGTGATAACGTCGATGCACGCGAGGGGAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGTGTTAAGTGAGAAAGGTTGTGAAGTTTCTTAAACAGCTAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAATAGCTCACTAGTCGAGAGACTTTGTGCCGATAATTCAACGGGACTAAAACACAATACCGAAGCTACGGGCAGAAATGCGTTAGGAGAGCGTTGTAAGGGCTTAGAAGCCAGACCGTGAGGACTGGTGGAGCGCTTACAAGTGAGAATGCCGGTATGAGTAACGATTCAGAGTGAGAATCTCTGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCGGGTCCTAAGACGAGGCCGAAAGGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTTAACATGTGATGGAGTGACGGGGAAGGATAATTTTACCACTAATTGGATTGTGGGGTAAGTAACAACTGGGTTATGTAGGCAAATCCGCATAACTTAACTGGGAGTTACGACGCATAGCAAAAGGGCAACTGAGTAGCGAATTAGATGATTTCATGCCTCTTAAAAAAGCTTCTAACATTAAGTGTTAGGAAACCCGTACCGAGAACGGACACACGTCCCCAAGATGAGTATTCTAAGGCGAGCGAGAAAACTATTGTCAAGGAACTCTGCAAATTCATCCCGTAAGTTCGCAAGAAGGGATGCCCACAATAAAATGTGGGCCGCAGTGAATAGTAAGGGGGAACTGTTTATCAAAAACACAGCTCTATGCTAAGTCGTAAGATGATGTATATGGGGTGACTCCTGCCCAGTGCCCGAAGGTTAAGCAGAGGTGTTAGCATTAGCGAAGCATTAATGTGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGAGTAATTATCTCTTAACTGTCTCGACAATAGACTCGGTGAAATTATGGTTCCGGCGAAGACGCCGGAGACCCGCATCTAGACGAAAAGACCCCGTGGAGCTTTACTATAACTTCATATTGGAGTTTGATTTAACATGTGTAGGATAGGTGGGAGACGTTGATGCTTGAACGCTAGTTTAAGAGTAGTCGCCGTTGAAATACCACCCTTGTTACATTGAACTTCTAACTTGTTCCCGTTATCCGGGAAGAGGACAGTGTGTGGTGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGGGTAACGGAGGCGTTCAAAGTTACACTCAATACGGTCAGAAACCGTATCTAAGAGCATAAAGGTAGAAGTGTGATTGACTGTGAGACCTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCGGTTCTTCGTGGAAAGGCCGTCGCTCAACGGATAAAAGCTACCCCGGGGATAACAGGCTTATCTTTCCCAAGAGATCACATCGACGGGAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTTCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCTGCTCTTAGTACGAGAGGACCGGAGTGGACGCACCGATGGTGTGCCAGTTGTTTCGCCAGAAGCATAGCTGGGTAGCCAAGTGCGGCAGGGATAACCGCTGAAAGCATCTAAGCGGGAAGCCCCCTCAAAGATTAGTATTCCCTTTAAAATTCCTTATAGACTATGAGGTTGATAGGCTGGAGGTGTAAGTGCAGCAATGCATTCAGCTGACCAGTACTAATAAATTTATTGGTTTAATAGT", "id": "DFOKDJOJIG_40", "locus": "GCA013367855_01006" }, { "type": "rRNA", "sequence": "contig_1", "start": 572591, "stop": 574108, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1510.8, "evalue": 3.1e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTATAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAGGTTAGCAATAACCTAGCGGCGAATGGGTGAGTAACACGTGCTTAATCTACCTTTTAGATTGGAATACCCATTGGAAACAATGGCTAATGCCGGATACGCATGGAACCGCATGGTTCCGTTGTGAAAGGCGCTGCAAGGCGTCACTAAAAGATGAGGGTGCGGAACATTAGTTAGTTGGTGAGGTAATGGCCCACCAAGACTATGATGTTTAGCCGGGTCGAGAGACTGAACGGCCACATTGGGACTGAGATACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCACGATGAAGGTCTTCGGATTGTAAAGTGCTGTTATAAGGGAAGAACATTTGCAATAGGAAATGATTGCAGACTGACGGTACCTTGTCAGAAAGCGATGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTTCGTAGGCTGTTTGTTAAGTCTGGAGTTAAATCCCGGGGCTCAACCCCGGCTCGCTTTGGATACTAGCAAACTAGAGTTAGATAGAGGTAAGCGGAATTCCATGTGAAGCGGTGAAATGCGTAGATATATGGAAGAACACCAAAGGCGAAGGCAGCTTACTGGGTCTATACTGACGCTGAGGGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTCGGTGGAGAATCACTGACGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACACGGAAAACCTTACCCACTCTTGACATCCTTCGCAAAGCTATAGAGATATAGTGGAGGTTATCGGAGTGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTGCAACGAGCGCAACCCCTATCTTTAGTTACTAACATTAAGTTGAGGACTCTAGAGATACTGCCTGGGTAACTGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCCACACACGTGCTACAATGGTCGGTACAAAGAGAAGCAATATGGCGACATGGAGCAAATCTCAAAAAGCCGATCTCAGTTCGGATTGGAGTCTGCAATTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCTATGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATACCCAAAGTCGGTTTGCTAACCTCGGAGGCGACCGCCTAAGGTAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "DFOKDJOJIG_36", "locus": "GCA013367855_01007" }, { "type": "cds", "sequence": "contig_1", "start": 574384, "stop": 574611, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATAAAGAAGCAATGTTGCTAGCTAAATTTAAAGCAGGGGACATTGAAACTGCTTTTAAAGAAGGGGCTTTAGTAAAAATTTCTAGTGGAATATTCAAAGATGAAGTTGGCGAAATAATTAAAAATAATGATATTACCCAAAAAGCCTTTGTTAATATTGAACAATTTGGTAGAAAAGTTCCAACTGAATTTGATTATGCAGATCTAGAAATAGTAGGTTAA", "aa": "MIDKEAMLLAKFKAGDIETAFKEGALVKISSGIFKDEVGEIIKNNDITQKAFVNIEQFGRKVPTEFDYADLEIVG", "aa_hexdigest": "6cd0e41740e267b0c0a651441ba51b2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8339.500699999999, "isoelectric_point": 4.7058935165405265 }, "id": "DFOKDJOJIG_1012", "locus": "GCA013367855_01008" }, { "type": "cds", "sequence": "contig_1", "start": 574708, "stop": 574938, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02357.24" ], "nt": "ATGATTTCAACAGTTTCAGGCAAAGAAGAAAATGTTATAGAATCATTAAAGAATAAAATTGCTGCTCAAAGAATGGATGAATACTTTAAAGATATAAGGATGTTTAAAGAACCCCATTTATCAAATAAAGAATTAGAAAAGAAGCAAAGAAACGAAGAATTTACTGTTAAATTTGTAAATATGTACAAAGGTTATATTTTCTTAAATATGGCAATGACTGATGATACATGA", "aa": "MISTVSGKEENVIESLKNKIAAQRMDEYFKDIRMFKEPHLSNKELEKKQRNEEFTVKFVNMYKGYIFLNMAMTDDT", "aa_hexdigest": "83445adab5e4fdbba14afdcaba235a99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02357.24", "name": "NusG", "length": 74, "aa_cov": 0.9736842105263158, "hmm_cov": 0.7432432432432432, "evalue": 2.6570551466178647e-08, "score": 29.945831298828125, "start": 2, "stop": 75 } ], "seq_stats": { "molecular_weight": 9073.367599999994, "isoelectric_point": 6.579306602478028 }, "id": "DFOKDJOJIG_1013", "locus": "GCA013367855_01009" }, { "type": "cds", "sequence": "contig_1", "start": 575666, "stop": 576103, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5D951C", "UniRef:UniRef90_UPI001E5D951C" ], "nt": "TTGAACAATGATGAATGCAAGGAACTAGAAAAACAAATTGGAATTAATTTGCATGAAGTTTGCAGTTTATATAAGGCATGTAAAATATTAAAAACCAAAGTAGATGATATTGTTTATTTGCATATGAATAAGCATACTTTTTGGCATTTAAAATGCACAATTCTTATTAAAAATAAATATAAAGAAATTTATTTTAAAAGACTAGAAATTTTTAATTTACTTGAAACCATCTTTAAAAAAGTCGCAGAAATTGATAAAAAAAGTGGAAATATACAAAAAATCGTAAATAAATACAAAGTTAGTTTAAATGATTTATCAAATATAATGGCAGGCGAACATGAACATTTTCCTCAACACTTCATTGATAATTTAAAAGACAAAAAAACAATTATACAAAAAAAATTAGTTGATTTTAATAATGATGAATATCTAAAATGA", "aa": "MNNDECKELEKQIGINLHEVCSLYKACKILKTKVDDIVYLHMNKHTFWHLKCTILIKNKYKEIYFKRLEIFNLLETIFKKVAEIDKKSGNIQKIVNKYKVSLNDLSNIMAGEHEHFPQHFIDNLKDKKTIIQKKLVDFNNDEYLK", "aa_hexdigest": "feec6b386539fcb46542228b26e31640", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 575588, "stop": 576203, "observations": { "insertion": [ 576182 ], "mutation": [ 575612, 576101 ], "stop": [ 575612, 576101 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E5D951C", "query_cov": 1.0, "subject_cov": 0.7107843137254902, "identity": 0.972, "score": 288.0, "evalue": 6.88e-98, "gene_start": 1, "gene_end": 145, "reference_start": 27, "reference_end": 171, "reference_sequence": "MDNFINKIWTFINKAKNSYDKICQLSLNNDECKELEKQIGINLHEVCSLYKACKILKTKVDDIVFLHMNKHAFWHLKCTILIKKKYKEIYFKRLEIFNLLETIFKKVAEIDKKSGNIQKIVNKYKVSLNDLSNIMAGEHEHFPQHFIDNLKDKKTIIQKKLVDFNNDEYLKWNEKILKQNLLEIKNLIIEYAAFYFKFKTITRI" }, "paralog": false, "identity": 0.9656862745098039, "score": 394.0, "evalue": 6.56e-142, "description": "Internal stop codon at 575612, 576101. Frameshift due to insertion around 576182. Nonsense mutation around 575612, 576101.", "psc": { "uniref90_id": "UniRef90_UPI001E5D951C", "uniref50_id": "UniRef50_UPI001E5D951C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E5D951C", "UniRef:UniRef50_UPI001E5D951C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E5D951C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5D951C" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17329.1342, "isoelectric_point": 9.023591423034667 }, "id": "DFOKDJOJIG_1014", "locus": "GCA013367855_01010" }, { "type": "cds", "sequence": "contig_1", "start": 576287, "stop": 576640, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGAAAAAGAATTTAAATTTAATTATGAGATAGAAGTTGAAAAAATAAAAACTTTGATTAATAAATGTCAAAATTCAAAAGACATTTCAAATATTGAACAAATTATTAATAAACTATTAGCTTCGATAAATACGACTAGTATAAAAATATATAACTATATTAAAACCTTTGCTTTTCATTTAAAAATACAAAATAATATAAAAAAAATCATTGAAAAAATTGACATTCAAAATAAAAAAATTTCTGATTTCTTTGCAAGCAAAACTAAAAAGAATTTGCTAAAAAGTGAGTATTTTAATGTCTATAAGCCAATATTGAATAGCTTTATAGATATTAAAAATAATAATTGA", "aa": "MLEKEFKFNYEIEVEKIKTLINKCQNSKDISNIEQIINKLLASINTTSIKIYNYIKTFAFHLKIQNNIKKIIEKIDIQNKKISDFFASKTKKNLLKSEYFNVYKPILNSFIDIKNNN", "aa_hexdigest": "54efd23d193b4ec1617cdf9ecd91d0fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13898.107399999975, "isoelectric_point": 9.583950614929197 }, "id": "DFOKDJOJIG_1015", "locus": "GCA013367855_01011" }, { "type": "cds", "sequence": "contig_1", "start": 576689, "stop": 577129, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410F5F0", "UniRef:UniRef90_UPI002410F5F0" ], "nt": "TTGAATTTGTTGAAGATTCAAAATAAAATAATAAATAAAATAGACGAACAAGAATATCAAAACAATCAAATTGAATTTTATATTGAAAACTTTAAAAGATTAACTGCCAATATAAAAGTATTAAACATTAATTTAAATCCAATAGACATTGAAAACATAAATAGTTTATTTACTTTTATTTATAAATTCAAAAATAATAATTTTTCTGAAAACGAATTGAATGACTTTTTAAGTTATTTATCTAATGAAATAAATTTTTTAAATTGCAAAATTCAATATTTTTTAATAATTAAAAATGTCGAATTTTCTTTAAGAAAAGACGTAAGTGAAAATTCAAACGCCCAAAATTTATTTAATGAGTTGCAAAAACTTAAAGGTTATAATGATGATAAAAAAGCAATCGATTTAATATATGATTATATAGAAGAACAATACAACTAA", "aa": "MNLLKIQNKIINKIDEQEYQNNQIEFYIENFKRLTANIKVLNINLNPIDIENINSLFTFIYKFKNNNFSENELNDFLSYLSNEINFLNCKIQYFLIIKNVEFSLRKDVSENSNAQNLFNELQKLKGYNDDKKAIDLIYDYIEEQYN", "aa_hexdigest": "0b6aabdf9601ee3669fc99d242f31b40", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 576389, "stop": 577129, "observations": { "mutation": [ 576638, 576674 ], "stop": [ 576638, 576674 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410F5F0", "query_cov": 1.0, "subject_cov": 0.5593869731800766, "identity": 0.925, "score": 259.0, "evalue": 1.27e-85, "gene_start": 1, "gene_end": 146, "reference_start": 116, "reference_end": 261, "reference_sequence": "MINKCQNSKDISNIEQIINKLLALINTTSIKIYNYIKTFNFHLKIQNNIKKIIEKIDVQNKKISDFFASETKKNLLKSEYLNTYKPILNSFIDIKNNNWMDIVEFIELKNWLESLLNLLRIQNKIINKIDEQEYRNNQIEFYIENFKRLTANIKILNISLSSEDIENINNLFAFIYKFKNNNFSANELNDFLSYLSNEINFLNCKIQYFLIIKNVEFSLRKDVSENSNAQNLFNELQKLKGYNDDKKAIDLIYDYIEEQYN" }, "paralog": false, "identity": 0.9227642276422764, "score": 448.0, "evalue": 2.64e-162, "description": "Internal stop codon at 576638, 576674. Nonsense mutation around 576638, 576674.", "psc": { "uniref90_id": "UniRef90_UPI002410F5F0", "uniref50_id": "UniRef50_UPI002410F5F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410F5F0", "UniRef:UniRef50_UPI002410F5F0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410F5F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410F5F0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17588.651499999993, "isoelectric_point": 4.830768775939941 }, "id": "DFOKDJOJIG_1016", "locus": "GCA013367855_01012" }, { "type": "cds", "sequence": "contig_1", "start": 577140, "stop": 577238, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22025.1" ], "nt": "ATGGACTACAATAAAATTTTAATTAGATATGGCGAATTGACTCTTAAAGGTGAAAATAAAAAGGATTTTATTAGGCAATTGCATAACAACCTATCTTGA", "aa": "MDYNKILIRYGELTLKGENKKDFIRQLHNNLS", "aa_hexdigest": "fadac77c9c73137a76016e2ce1732acf", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22025.1", "name": "ThiI_fer", "length": 26, "aa_cov": 0.8125, "hmm_cov": 1.0, "evalue": 5.503335478543502e-12, "score": 41.67063522338867, "start": 6, "stop": 31 } ], "seq_stats": { "molecular_weight": 3865.4184000000005, "isoelectric_point": 9.301772499084471 }, "id": "DFOKDJOJIG_1017", "locus": "GCA013367855_01013" }, { "type": "cds", "sequence": "contig_1", "start": 577467, "stop": 578288, "strand": "+", "frame": 3, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "SO:0001217", "UniRef:UniRef50_B3PLZ2", "UniRef:UniRef90_D1J8J1" ], "nt": "ATGACTTCAAGTGAATTAAATATGTATTTTGGGGGTATTGTTTTAAAACAAATGCCTGACAAACAAGTTAAATTAAAAAATCCTGATTTAACTATATATATTGAAATAAGAGATCAACGTAGCTTTGTTTTTTGCGATTATTTTGACGGCTTAGGTGGCATGCCGGTATTAAGTTCTGGAAAATGCATACATCTAATTAGTGGAGGTATTGATTCACCTGTAGCAGCGAATTTATTACAAAAAAGAGGCTTAAAAATAGTGTTTTTAAATTTTATTACCCCTCCTCAAACTGATGAAAAAACAATCGCAAAAATAGATGAAATTGTTAATATACTAAAAAAATATCAAGGCAATGCTATGTTATATCAAATTAACTATAGCAAAGTGATGGAATATATTGGTCTTATAAGCAATCAAAAATACAAAATAACTTTAATGAGAAGATCATTTTATAGAATAGCAAATCTTATTGCAAAAAAAGAAAACATTAAAGCAATTTCAAATGGCGAAAATTTAGCCCAAGTTGCCTCTCAAACTCTTGAATCTATTTATACTATCTCCGAAGTTTGTGATTTGCCTATTTTTAGACCTTTAATTTCTTTTGACAAAAATGAAACTATTGCTATAGCAAAAAAAATTAACACATTAGATGTTTCAATAGTAAGGGCGTGTGAAACATGTGAATTATTTGCTCCTAAAAATCCTGTAACCAAGCCTAATAGACAAGAAGCTCATAAACTTGAAGAAGAAGAATTGCCTTTGATAAAAGAATTTGAAGAAGAATGTATTAAAAATGCTATTATAAAAAAATATTCAATATAA", "aa": "MTSSELNMYFGGIVLKQMPDKQVKLKNPDLTIYIEIRDQRSFVFCDYFDGLGGMPVLSSGKCIHLISGGIDSPVAANLLQKRGLKIVFLNFITPPQTDEKTIAKIDEIVNILKKYQGNAMLYQINYSKVMEYIGLISNQKYKITLMRRSFYRIANLIAKKENIKAISNGENLAQVASQTLESIYTISEVCDLPIFRPLISFDKNETIAIAKKINTLDVSIVRACETCELFAPKNPVTKPNRQEAHKLEEEELPLIKEFEEECIKNAIIKKYSI", "aa_hexdigest": "9f6dbfd07aa2514523a9b9f308f8ac8b", "start_type": "ATG", "rbs_motif": null, "genes": [ "thiI" ], "truncated": "5-prime", "pseudogene": { "start": 577167, "stop": 578288, "observations": { "mutation": [ 577236 ], "stop": [ 577236 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8J1", "query_cov": 1.0, "subject_cov": 0.7146596858638743, "identity": 0.9890000000000001, "score": 529.0, "evalue": 4.34e-188, "gene_start": 1, "gene_end": 273, "reference_start": 110, "reference_end": 382, "reference_sequence": "MDYNKILIRYGELTLKGENKKDFIRQLHNNLSWYIEKSEIKTEYDRAFVDYSQKNLDCLKYIFGISSYSPVYETETSLINIENIVKEIISKKQFDSFAINARRHNKNFSMTSSELNMYFGGIVLKQMPDKQVKLKNPDLTIYIEIRDQRSFVFCDYFDGLGGMPVLSSGKCIHLISGGIDSPVAANLLQKRGLKIVFLNFITPPQTDEKTIAKIDEIVNILKKYQGNAMLYQINYSKVMEYIGLISNQKYKITLMRRSFYRIANLIAKKENIKAISNGENLAQVASQTLESIYTISEVCDLPIFRPLISFDKNETIAIAKKINTLDVSIVRACETCELFAPKNPVTKPNRQEAYKLEEEELPLIKEFENECIENAIIKKYSI" }, "paralog": false, "identity": 0.9892761394101877, "score": 728.0, "evalue": 6.5e-269, "description": "Internal stop codon at 577236. Nonsense mutation around 577236.", "psc": { "uniref90_id": "UniRef90_D1J8J1", "gene": "ThiI", "product": "Probable tRNA sulfurtransferase", "uniref50_id": "UniRef50_B3PLZ2", "cog_id": "COG0301", "cog_category": "HJ", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8J1", "UniRef:UniRef50_B3PLZ2", "COG:COG0301", "COG:HJ" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLZ2" ], "product": "Probable tRNA sulfurtransferase" } }, "id": "DFOKDJOJIG_1018", "locus": "GCA013367855_01014" }, { "type": "cds", "sequence": "contig_1", "start": 578338, "stop": 578451, "strand": "-", "frame": 1, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920J7V4" ], "nt": "ATGCCTCCAACAGGCGGTTCTGGTATAGGAATTGATCGTTTAATTATGCTACTAACTCAAAATGATTCAATTAGAGATGTAGTTTTATTCCCTCAATTAAAAGATTTAAAATAA", "aa": "MPPTGGSGIGIDRLIMLLTQNDSIRDVVLFPQLKDLK", "aa_hexdigest": "d9b52268f805181a0d249c6481f62c51", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A920J7V4", "query_cov": 0.8378378378378378, "subject_cov": 0.8611111111111112, "identity": 0.677, "score": 46.2, "evalue": 5.74e-06, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A920J7V4" }, "pscc": { "uniref50_id": "UniRef50_A0A920J7V4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920J7V4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_1019", "locus": "GCA013367855_01015" }, { "type": "cds", "sequence": "contig_1", "start": 578479, "stop": 578976, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGGAAAGAACTGAAGGTGTTTTCAAACATTTAATAAATAAATTCAACATTAAAGAAGTTAAATTTAATGGTTTTGATATAACATTTAATTTTCCATTTAAGAGAGTCAATATGATTGATGCTATATCGGAAAAAATTAATGTTGACATTAGAAAATTAAATGAAGAACAAATGTTTGAATTAGCTAAAAAACATGGCATTAAAATTGAAAAATTCTTTAGATATGGTCACATTATCAATGAATTATTTGAAAAATATGTTGAAGAAACATTAATAGAACCAACATTTGTATATGGCCACCCAATTGAAATTTCTCCTCTTGCAGCAATTGACCCTGATGATAAAAGATTTACACTACGTGCTGAACTATTTATTGGAACAAAAGAATTTGCAAATATGTTTACTGAATTAAATGATCCAATTGACCAATTGCAACGTTTTGAAAACCAATTGTTGGAAAAAGAAAATGGTAATGAAGAAGCAAATGAAATTGATTGA", "aa": "MERTEGVFKHLINKFNIKEVKFNGFDITFNFPFKRVNMIDAISEKINVDIRKLNEEQMFELAKKHGIKIEKFFRYGHIINELFEKYVEETLIEPTFVYGHPIEISPLAAIDPDDKRFTLRAELFIGTKEFANMFTELNDPIDQLQRFENQLLEKENGNEEANEID", "aa_hexdigest": "49f461e13a235e2b9da7d3923829e779", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0027D2801B", "query_cov": 0.9030303030303031, "subject_cov": 0.8097826086956522, "identity": 0.523, "score": 147.0, "evalue": 3.41e-42, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_A0AA38HFP6" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 165, "aa_cov": 0.9636363636363636, "hmm_cov": 0.806060606060606, "evalue": 8.746018555994525e-15, "score": 50.15831756591797, "start": 1, "stop": 159 } ], "seq_stats": { "molecular_weight": 19565.068399999993, "isoelectric_point": 4.986791801452636 }, "id": "DFOKDJOJIG_1020", "locus": "GCA013367855_01016" }, { "type": "cds", "sequence": "contig_1", "start": 578983, "stop": 579804, "strand": "-", "frame": 1, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EED3E5F" ], "nt": "ATGGAAAGAAAACTTAGTGAACAAGAAGAAATTAGACGTAATAAAATTGCTGAATTAGAAAAAAACAATTTACAAGCATTTCACAAAACTATAAAACCAACAACAAATTCAAAAGAGTTAAATGAAAAATATAAAAATTTAAGTCGTGAAGATCTTGACAAACAAAATCTAGAATTTGTATTTAATGGAAGGATTATGACTATTCGTGGTCCATTTATTGTTTTACAAGATAATTGCGGACAAATGCAAATTTATGCAAACAAAAAAACATTGAATGAGTCTTCTGCAAAAACCTTTTCTTTAATGGATTTTGGCGATATTTTAAGTGTAAGAGGAACATTGTCAAAAACTAATACTGATGCTTTGGTTATCAAAGCTCAAGACATAGTTCTATTAACAAAATCATTGAAACCCTTACCTGATAAATTTCATGGTTTAGTTGATCCAGAAGAAAGACGTCGTCAACGCTATGTAGATTTAATAATGAATGAAAAAACAAGAAATACATTTGTTATGAGAACCCAAATTATCAAAGGCATTAGAGAATATTTTGATAGTTTGAACTACATTGAAGTTGATACACCAATTTTAAATCCAATATTAGGAGGAGCTGCTGCCAAACCTTTTATTACTTTCTATAATGCACTTAATAGAAACTTTTATTTAAGAATTGCAACTGAATTGCCATTAAAAAAATTGATTGTTGGTGGAATGGATAGAGTTTATGAAATAGGAAGAATATTTAGAAATGAAGGCGTAGATACAACTCATAATCCAGAATTTACCTCAATTGAATTTTATGAAGCATATTCAGATTTATGA", "aa": "MERKLSEQEEIRRNKIAELEKNNLQAFHKTIKPTTNSKELNEKYKNLSREDLDKQNLEFVFNGRIMTIRGPFIVLQDNCGQMQIYANKKTLNESSAKTFSLMDFGDILSVRGTLSKTNTDALVIKAQDIVLLTKSLKPLPDKFHGLVDPEERRRQRYVDLIMNEKTRNTFVMRTQIIKGIREYFDSLNYIEVDTPILNPILGGAAAKPFITFYNALNRNFYLRIATELPLKKLIVGGMDRVYEIGRIFRNEGVDTTHNPEFTSIEFYEAYSDL", "aa_hexdigest": "c89eb4f6d13bb8cc3900c39be10e7b18", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001EED3E5F", "query_cov": 0.9926739926739927, "subject_cov": 0.8885245901639345, "identity": 0.542, "score": 290.0, "evalue": 5.3e-95, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_UPI001EED3E5F", "kegg_orthology_id": "K04567" }, "pscc": { "uniref50_id": "UniRef50_UPI001EED3E5F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EED3E5F" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "DFOKDJOJIG_1021", "locus": "GCA013367855_01017" }, { "type": "tRNA", "sequence": "contig_1", "start": 579896, "stop": 579970, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 68.0, "nt": "GGTCGCATAGCTCAGTGGAAGAGCACGAGCCTCCTAAGCCCGGGGTCGCAGGTTCAACTCCTGTTGCGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 579925, 579927 ], "id": "DFOKDJOJIG_23", "locus": "GCA013367855_01018" }, { "type": "cds", "sequence": "contig_1", "start": 580241, "stop": 580423, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGACATCCAATCGTTCTTTGGCAATGAACAAGGAATGAATTTTGTTGAACTTGATGAAAGTAAAAGAAAAGCTCACATATTTTATGCCAAAATTTTCTTTATAAGTATCGCAGTTTTAATTGTATTGCCCTTTATAATATATATTATTTTAAAGAAGACAGTATTGAAAGGAAAGTAG", "aa": "MVDIQSFFGNEQGMNFVELDESKRKAHIFYAKIFFISIAVLIVLPFIIYIILKKTVLKGK", "aa_hexdigest": "5a4badaa5c8b1817fbaff71a052f1d35", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6966.339399999997, "isoelectric_point": 9.45623874664307 }, "id": "DFOKDJOJIG_1022", "locus": "GCA013367855_01019" }, { "type": "cds", "sequence": "contig_1", "start": 580731, "stop": 580880, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTATGTATTCCTGAAAGTTCAATCAATTCAATAATAAATCTAGTTAACGAAATTACTATAAATATTGCCGGAATAATATATCAAGCCCATTTTATTTTAAAATCTTTTGCATTAGAAAAATTGAATAAATTTGGTCAAAAGTAA", "aa": "MLLCIPESSINSIINLVNEITINIAGIIYQAHFILKSFALEKLNKFGQK", "aa_hexdigest": "3ae2d7ac31707d9ab75aeff189165935", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5504.507899999999, "isoelectric_point": 7.923373985290528 }, "id": "DFOKDJOJIG_1023", "locus": "GCA013367855_01020" }, { "type": "cds", "sequence": "contig_1", "start": 580925, "stop": 581146, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001CC77350", "UniRef:UniRef90_UPI001CC77350" ], "nt": "ATGGATATTAAACCCACAACAACAGGAAATAATAGCGATAGTAGTAAAAACATTGAAATTCCAACACGTACCTTTGAAAATCACATGGTAGAAGATTCAATGAATACTCAAAATTTGCAACAATTAAGTTATAAAATAAAAGATCCTCAGAAAATAATTCCTAATGGAATTTATTCAGTTTTTCAAAGAGAAATGAATATTAAAAAAATATCAATACTTTGA", "aa": "MDIKPTTTGNNSDSSKNIEIPTRTFENHMVEDSMNTQNLQQLSYKIKDPQKIIPNGIYSVFQREMNIKKISIL", "aa_hexdigest": "196c3e50192ffbffea27cce0ba74613e", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 580792, "stop": 581146, "observations": { "insertion": [ 580822 ], "mutation": [ 580873, 580927 ], "stop": [ 580873, 580927 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001CC77350", "query_cov": 1.0, "subject_cov": 0.6239316239316239, "identity": 0.986, "score": 141.0, "evalue": 4.66e-42, "gene_start": 1, "gene_end": 73, "reference_start": 1, "reference_end": 73, "reference_sequence": "MDIKPTTTGSNSDSSKNIEIPTRTFENHMVEDSMNTQNLQQLSYKIKDPQKIIPNGIYSVFQREMNIKKISILWNFGILLLSVIAILLLYFWPNLFNFSNAKDFKIKWLDILFRQYL" }, "paralog": false, "identity": 0.9743589743589743, "score": 220.0, "evalue": 8.33e-76, "description": "Internal stop codon at 580873, 580927. Frameshift due to insertion around 580822. Nonsense mutation around 580873, 580927.", "psc": { "uniref90_id": "UniRef90_UPI001CC77350", "uniref50_id": "UniRef50_UPI001CC77350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001CC77350", "UniRef:UniRef50_UPI001CC77350" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001CC77350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001CC77350" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8428.521199999997, "isoelectric_point": 8.030133628845213 }, "id": "DFOKDJOJIG_1024", "locus": "GCA013367855_01021" }, { "type": "cds", "sequence": "contig_1", "start": 581153, "stop": 581917, "strand": "-", "frame": 3, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003924", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q9ZHZ8", "UniRef:UniRef90_A0A454CA39" ], "nt": "ATGGATATTCTTCAAGAGAGACTCGAAAGAGAATTTAATTTAGATGTTATTGCTACTGCTCCTTCAGTTGAGTTTGTTGTAACACTAACTAATGGAGATGTTCAATATATTACAAATCCATCACTATTTCCAGATAGAAGTTATATAAAAATGATAGAGGAACCTTATATTAAGGCTAACATAATGCTAACCGAAGAATATTTGGGCCCAATTATGGAATTATGTCAATCTAAACGTGGCAAATATGTAAATATTGAATATTTAGATGAAACACGTAGATTATTGATTTATGAATTGCCTTTAAATGAAATAATATTTGATTTCTTTGATTTAATGAAGAGCTATTCAAAAGGCTATGCTTCATTTGATTATGACTACATTGGTTTAAGAGAAAGCGATTTAGTAAAGGTTGATATTTTATTAAATGGTGAGAAAATTGATGCCTTAGCAATGATTGTTCACAGAGATTCAGCATATAATAAAAGTAGAGCATTAACAGAAAAACTAAAGGAAGTAGTACCAAGACAAAATTTTGAAATTCCCGTTCAGGCTGCAATTGGTGCTAAAATTATAGCAAGAGAAACAATTAAGGCATATAGAAAAGATGTTACAGCTAAGTTATATGGTGGTGACGTTACAAGAAGACAAAAACTATTAAAAAAACAAAAAATAGGTAAAAAAAGGATGAAACAAATAGGAAGCGTTGAAGTTCCACAAGAGGCATTTTTGGCAATATTAAAAACAGATGATTCTCGAAAGGATTAG", "aa": "MDILQERLEREFNLDVIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHRDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD", "aa_hexdigest": "5f62ef568a7c96d13b04d8576a2cea48", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449BBE2", "query_cov": 1.0, "subject_cov": 0.8639455782312925, "identity": 0.685, "score": 345.0, "evalue": 7.04e-117, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A449BBE2" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 581153, "stop": 582217, "observations": { "mutation": [ 581983, 582190 ], "stop": [ 581983, 582190 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA39", "query_cov": 1.0, "subject_cov": 0.4240400667779633, "identity": 0.996, "score": 489.0, "evalue": 2.2e-169, "gene_start": 1, "gene_end": 254, "reference_start": 346, "reference_end": 599, "reference_sequence": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDTSDAIMISAKTGLNIDKVIHAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTEKHYLVTELGVKNPNEVQKNELQAGEVGWISASIRDIKDVEVGDTVTLFENQCPEPLPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLVWQPETSKALGFGFRIGFLGLLHMDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHRDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD" }, "paralog": false, "identity": 0.9915254237288136, "score": 687.0, "evalue": 1.18e-249, "description": "Internal stop codon at 581983, 582190. Nonsense mutation around 581983, 582190.", "psc": { "uniref90_id": "UniRef90_A0A454CA39", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q9ZHZ8", "go_ids": [ "GO:0003924", "GO:0005525" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA39", "EC:3.6.5.n1", "UniRef:UniRef50_Q9ZHZ8", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZHZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZHZ8" ], "product": "Elongation factor 4" } }, "id": "DFOKDJOJIG_1025", "locus": "GCA013367855_01022" }, { "type": "cds", "sequence": "contig_1", "start": 581981, "stop": 582106, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAGGATATAAAAAAATAAAACCAGTTGTTTATACCGGATTTTATCCCGTTGATACTAGAGATTACAATGATTTAAAAGATGCCCTAGAAAAAATTAGTTTATCTGATTCTTCATTGGTATGA", "aa": "MPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLV", "aa_hexdigest": "4a49afffe937ca97bc817e164d8b878b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4667.291399999999, "isoelectric_point": 5.986134910583496 }, "id": "DFOKDJOJIG_1026", "locus": "GCA013367855_01023" }, { "type": "cds", "sequence": "contig_1", "start": 582188, "stop": 582952, "strand": "-", "frame": 3, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003924", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q9ZHZ8", "UniRef:UniRef90_A0A454CA39" ], "nt": "ATGACAAACGAAAAAATAAGAAATTTTGCAATAATAGCACACATTGATCACGGTAAAAGTACTTTAGCTGATCGTATTTTGGAATTTACCAATACAATTTCAGCCAGGGAAGCAAAACCTCAAATTTTAGATTCAATGGAACTAGAACAAGAACGTGGAATCACTATTAAATTAAATGCTATTCAAATTAAATATAAAGATTACATTTTTCACCTGATAGATACACCAGGTCATGTTGATTTTACTTATGAGGTTAGTAGATCACTTGCTGCATGTGAAGGAGCATTGTTGCTAGTTGATGCAACTCAAGGAATTCAAGCACAAACATTGGCTAATGTTTATATGGCTATGGAAAATAATTTAGAAATCGTTCCTATTATTAATAAGGTAGATTTAATTAGTGCAGATCCTGAAAGAGTAAAAAAAGAAATTCAAGACATGATTGGAATTGATACTTCTGACGCAATTATGATTTCTGCAAAGACAGGATTAAATATTGATAAGGTAATTGATGCAATTATAAACAAAATTCCTGCTCCTAAAGAAGCAGATATAAATAAACCATTGCAAGCTTTAATTTTTGATAGCTATTTTGACAATTATCGTGGCGTTGTTATCTATGTGAGATTGTTTGACGGCAAAGTAAAAGTAGGCGACGAAATTTACTTTATGCAAACAAAAAAGCATTATTTAGTAACTGAATTAGGCGTTAAAAACCCTAATGAAGTTCAAAAAAATGAATTGCAAGCTGGCGAAGTTGGTTGA", "aa": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDTSDAIMISAKTGLNIDKVIDAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTKKHYLVTELGVKNPNEVQKNELQAGEVG", "aa_hexdigest": "db14056d5fb1586be72ee423a2d79036", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00317F3865", "query_cov": 1.0078740157480315, "subject_cov": 0.8827586206896552, "identity": 0.629, "score": 323.0, "evalue": 2.82e-108, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A0P1NWJ7" }, "genes": [ "lepA" ], "truncated": "5-prime", "pseudogene": { "start": 581885, "stop": 582952, "observations": { "mutation": [ 581983, 582190 ], "stop": [ 581983, 582190 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA39", "query_cov": 1.0, "subject_cov": 0.4240400667779633, "identity": 0.992, "score": 489.0, "evalue": 2.2e-169, "gene_start": 1, "gene_end": 254, "reference_start": 1, "reference_end": 254, "reference_sequence": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDTSDAIMISAKTGLNIDKVIHAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTEKHYLVTELGVKNPNEVQKNELQAGEVGWISASIRDIKDVEVGDTVTLFENQCPEPLPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLVWQPETSKALGFGFRIGFLGLLHMDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHRDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD" }, "paralog": false, "identity": 0.9887323943661972, "score": 686.0, "evalue": 4.78e-249, "description": "Internal stop codon at 581983, 582190. Nonsense mutation around 581983, 582190.", "psc": { "uniref90_id": "UniRef90_A0A454CA39", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q9ZHZ8", "go_ids": [ "GO:0003924", "GO:0005525" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA39", "EC:3.6.5.n1", "UniRef:UniRef50_Q9ZHZ8", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZHZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZHZ8" ], "product": "Elongation factor 4" } }, "id": "DFOKDJOJIG_1027", "locus": "GCA013367855_01024" }, { "type": "cds", "sequence": "contig_1", "start": 583053, "stop": 584540, "strand": "-", "frame": 2, "gene": null, "product": "endopeptidase La", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0U7" ], "nt": "TTGAATAAGAATCACTATGGACTTGAAAAGCCAAAGGAAAGAATTCTTGAATTTATTTCGGTTTTAACTCATCTTAAAATCAAGGAAGAAAAAACTGAATATTTACCAATAAAAGGAAATGATCAAGAATTTATTGATACTAAATTATTCGTTCATAAAACGGGTCAAACCACAAATAAACCAGTAAATAATATTCCTATTTTAACATTAATTGGTCCCCCAGGAACTGGTAAAACTACACTTGCTAAATCAATCGCCGAATCACTAAATCGTAGATTTATAAAGGTTTCATTGGGCGGTGTTAAGGATGAAAGCGAAATAAGAGGACACAGAAGAACTTATGTTGGGGCAATGCCTGGAAAAATAATATCAGCAATTAAAAAAGCTGGAGTTTCAAACCCAGTCATTTTGTTAGATGAAATAGATAAGATGTCATCAGATTTTCGAGGCGATCCAACAAGCGCAATGCTTGAAGTGTTAGACCCTGAACAAAATGTTAATTTTCAAGATCACTACTTGGATTTAGAATACGACTTATCAAAAGTATTATTCATCGCTACTGCTAACTACTATGATTCAATACCAGCAGCACTAATTGATCGTGTTGAATTGCTTGAACTTTCAACATATACTACTAAAGAAAAAGTAGAAATTGCTAAATCACATTTACTTCCAAAAGTGTATGAACAAAATGCCTTAAATCCTGAACAATTTCAAATTTCTGACGAAGTTTTAGAATTTTTAGTTCGTTACTACACAAGGGAAAGTGGAGTTCGTGAATTAAAACGTATTTTAGATAGTATAGCAAGAAAGATTGTTGTCGAAATTCTTGACAAAAAAATCAACGATAGTTTTATTGTTACAAAAGAAATTGTAATTGAATTTTTAGGACCAATTAAATTCGATGATGATGAAAATGAAAATAAGGCACAAATAGGTGTTGTTAATGGTTTGGCATATACAAGTTTTGGTGGTTCAACATTAGCTATTGAAGTTACAACATTCCCTGGAAAAGAAGGCCTAAAAATGACAGGGCAACTAAAAGATGTTATGCGTGAATCTGCTCAAATTGCATTAGCTTATGTAAGAGCAAATGCTGCCAAATTTAATATTGACTTTGATTTTGAAAATACAGCAATTCATATTCACGTTCCAGCAGGTGCTATTCCAAAAGATGGCCCAAGTGCCGGAGTAACATTTACAACCTCAATAATTAGTGCCTTATCTAAAAGACCAGTCCCTGCTAATATCGGTATGACTGGAGAAATTACATTGCGTGGAAAAGTTCTACCAATTGGTGGTTTAAAAGAAAAATCATTGGCGGCTGCTCAATTTGGTATTAAAAAGATTTTTATTCCATATGATAATTATAAGAATCTAAAAGATGTTCCTGCTGAAGTTAAAAATGTTGTTGAATTTGTGCCAGTTCATTATTATGACGAAATATTTGAACAAATATTTGGTGCCAAAGAAACAAAAAATAATTAA", "aa": "MNKNHYGLEKPKERILEFISVLTHLKIKEEKTEYLPIKGNDQEFIDTKLFVHKTGQTTNKPVNNIPILTLIGPPGTGKTTLAKSIAESLNRRFIKVSLGGVKDESEIRGHRRTYVGAMPGKIISAIKKAGVSNPVILLDEIDKMSSDFRGDPTSAMLEVLDPEQNVNFQDHYLDLEYDLSKVLFIATANYYDSIPAALIDRVELLELSTYTTKEKVEIAKSHLLPKVYEQNALNPEQFQISDEVLEFLVRYYTRESGVRELKRILDSIARKIVVEILDKKINDSFIVTKEIVIEFLGPIKFDDDENENKAQIGVVNGLAYTSFGGSTLAIEVTTFPGKEGLKMTGQLKDVMRESAQIALAYVRANAAKFNIDFDFENTAIHIHVPAGAIPKDGPSAGVTFTTSIISALSKRPVPANIGMTGEITLRGKVLPIGGLKEKSLAAAQFGIKKIFIPYDNYKNLKDVPAEVKNVVEFVPVHYYDEIFEQIFGAKETKNN", "aa_hexdigest": "7d5c5bbe5bd2c00fdd29dd7c449463a7", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0P0U7", "query_cov": 0.9838383838383838, "subject_cov": 0.9779116465863453, "identity": 0.856, "score": 821.0, "evalue": 1.18e-297, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A3B0P0U7", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0U7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0U7" ], "product": "endopeptidase La" }, "genes": [], "id": "DFOKDJOJIG_1028", "locus": "GCA013367855_01025" }, { "type": "cds", "sequence": "contig_1", "start": 584589, "stop": 584987, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAGTACTTTTTGAGTGATGAACTTCTTTCTTTAATTGAAAGCAATGACGTTTTAGCTAGATACAATGAACTAATTAAAAAGGCAAATAAGCAAGTTCAATTTGAAAAAATAGAAAACGAAATTAATAGTAATTTACGTGAAGATATGGAAGACCAACAAAGAGATTTCTTCCTAAGAGAAAAAATGCGCCAAATTAGAAAAATGTTGAAGGATGATGAAGGTGGAAAATCTGCTGACGACATTGAAAAAGACAGCACTTTAAAAGAACAATATCCTGAATATGTTCTAGAAGCGTTAAGATCTGAACAATCAAGAATGGCCTCAATGATGCCATCTAGCCCAGAAGCTAATATATCTAAAACATATATTGATTTAATCTTGGCATTGCCTTGA", "aa": "MKKYFLSDELLSLIESNDVLARYNELIKKANKQVQFEKIENEINSNLREDMEDQQRDFFLREKMRQIRKMLKDDEGGKSADDIEKDSTLKEQYPEYVLEALRSEQSRMASMMPSSPEANISKTYIDLILALP", "aa_hexdigest": "8caae9d413dc55a00fb0ae39141fdaf3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15544.501499999995, "isoelectric_point": 4.859586143493652 }, "id": "DFOKDJOJIG_1029", "locus": "GCA013367855_01026" }, { "type": "cds", "sequence": "contig_1", "start": 585003, "stop": 585536, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTACCTTATATAGCAATAAAAAATCAGATTATTATTCCATATACTTCTGACAAAATTAGAGTTGGAAAAGCTAAATCAGTATCAGCTTTATTAAAGGCTTTTAATGGAAATAAAAATATAGTTATTACATTTTTAAATCCAAATTATAAACAATCAAATGAGGAACATACAATTAATGATATTGCTCCTGTAGGCGTACTTGTTAATATTACTAACATTAGTGAAGAATCAGGAATTTATCATGTTAATTTAGAAGCAAAAGAACCGGTTAATATTAATAAAATCTCAATAGTTGATGAAAAATCAACAAATAGTGAGGATAATTTTTATGTTGATATTTCGCCTATTCAAGTCAATTCATATGATTCATATTTACCAAAAATTGAAGAAATAAGACAAAAGTTCTTCTCACTATCTAAACAAGTTATTAACGAAGGTATAGGAACAGTTTTATTTCGAGATTTAGAAAAAGAATTTGAAACTAACAAACCTAAACAATCATTAGTTGAATATGAAACTGAATCATGA", "aa": "MKLPYIAIKNQIIIPYTSDKIRVGKAKSVSALLKAFNGNKNIVITFLNPNYKQSNEEHTINDIAPVGVLVNITNISEESGIYHVNLEAKEPVNINKISIVDEKSTNSEDNFYVDISPIQVNSYDSYLPKIEEIRQKFFSLSKQVINEGIGTVLFRDLEKEFETNKPKQSLVEYETES", "aa_hexdigest": "ba49d13aa8d71dbd883dbac3ff060af5", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20099.549700000007, "isoelectric_point": 5.322482872009277 }, "id": "DFOKDJOJIG_1030", "locus": "GCA013367855_01027" }, { "type": "cds", "sequence": "contig_1", "start": 585603, "stop": 586391, "strand": "-", "frame": 2, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "KEGG:K03687", "RefSeq:WP_175972327.1", "SO:0001217", "UniParc:UPI001588A784", "UniRef:UniRef100_UPI001588A784", "UniRef:UniRef50_D1J8J6", "UniRef:UniRef90_D1J8J6" ], "nt": "ATGAGTCAAAATATTTCAAAATGCGATTATATAGAGTGCATTAGAAAAATTAGCAAAGATGAAAAAATTGAATATAAGGGATTTGTTGGATTTGATTTAGATAAAAATATTGAAAAAACCATCTTGGAAGGAAAGCAAAACGAAAAAGGTGAGTTAGTTTTAAAAATCGATAATGAAATTTTTAAGGTAATAATTGTTGATTTTCAAAAAACAAGTCCTAAGTATTTAGAAGTTTTTGCTAAAAATCAAGAATTGAATGAAAATATTAAAAAATTACAACTAAATTTTTTGGAATTAGGCGAACTTAATGAAAAAATAAAACAAGAAAAGACACAACAAGAAATTTTGTTTAAAAATCAAGTAATCGAATTGGAAGCAAAAGCACAATCAAAAATAAACGAACATCGCCAAAAAAATGATGAGCATTTATTGCAACAAAAAACAGAATTAAAAAAATATGCTCTACAAGACTTTTTAGAAGAGTTCATTAAGATATATACAAAATATGACAATGCTTTAAATTTTGCAAAAAAATCTGATAATATCGCTGTTAATAATTTTGCAAAAGGTTTTGATATGCTTAAGAATGATTTTGAAAATTTAATGTTAGATAACGGAATAAAAATAATTGAACCAAAAGTAGGAGATTTATTCGACCCCGAATGTCAACAAATTACTGAAAGCATTGAATCAAAAGAACCTTCTGGCACAATTCTTGAAGTAAAGAGCAATGGTTATTCATTATTTAACAGAATTTTAAAACCTGCTTCAGTAATAATTAGCAAATAA", "aa": "MSQNISKCDYIECIRKISKDEKIEYKGFVGFDLDKNIEKTILEGKQNEKGELVLKIDNEIFKVIIVDFQKTSPKYLEVFAKNQELNENIKKLQLNFLELGELNEKIKQEKTQQEILFKNQVIELEAKAQSKINEHRQKNDEHLLQQKTELKKYALQDFLEEFIKIYTKYDNALNFAKKSDNIAVNNFAKGFDMLKNDFENLMLDNGIKIIEPKVGDLFDPECQQITESIESKEPSGTILEVKSNGYSLFNRILKPASVIISK", "aa_hexdigest": "90649c6af97c6eff02a1034c2e11b017", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI001588A784", "ncbi_nrp_id": "WP_175972327.1", "uniref100_id": "UniRef100_UPI001588A784", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588A784", "RefSeq:WP_175972327.1", "UniRef:UniRef100_UPI001588A784" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588A784", "uniref90_id": "UniRef90_D1J8J6" }, "psc": { "uniref90_id": "UniRef90_D1J8J6", "gene": "GrpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_D1J8J6", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687" }, "pscc": { "uniref50_id": "UniRef50_D1J8J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J6" ], "product": "Protein GrpE" }, "genes": [ "grpE" ], "id": "DFOKDJOJIG_1031", "locus": "GCA013367855_01028" }, { "type": "cds", "sequence": "contig_1", "start": 586611, "stop": 587444, "strand": "-", "frame": 2, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "SO:0001217", "UniRef:UniRef50_B3PN01", "UniRef:UniRef90_D1J8J7" ], "nt": "ATGGAGCAAAATTCTTCTAATGCAAACACAAATAAGTCTTTAATTATATGCAATAATTATGATAAGAAACTAATCAAATATTTTAGATACATTGTTGAAATGTACATTAAAACCGGCGAACCAGTAGGATCAAAAAGATTAGTTGAAAAATTTAAATTATCTTGTAGCTCTGCAACTATAAGAAACGCAATGGCAGAATTAGAAAAACTAGGTCTTTTAGAAAAAAAACATGTTTCAGGAGGAAGAATTCCTTCTTCAAAAGGTCTTGAATACTATACAAAAAATTTACTTTATAATGCTAAAAAATATTTCAATGAAAAACTTGAAGATCTTTTATCTAAGCGTAGGTTAAGCATCAACACAACATTAGAAGAAGCAGCAAATGTCGTTAGCCAAATGGCAGGCTTTGCCGTAGTTGCAACTTCAAACAATGAATCTGAATTATTGAAAAGTATTCAGTTGACACCGCTTGATGAAGAATCTGCAGTTGTTGTTATTGTTACAAGTTCAGGAAATGTTCAAAACAAAATGTTTAAGTTTGAAAGTGGGATTGAATTAAATGATTTAAGGATTGCAATTAGATTATTTAAAGAAAGATTGATTGATACTCCATTAATTAATCTTGCTAAAAAAACCGAATCATTAGCCCCAATATTATCTACACAAGTTAAACATTTTGAATTAATAATGCAGAAATTTATCAAAAATATTTTTTGTTTTGAAGAAAAATTGGAGAATAAATCGTTTAATAAAAATGTAATAATATTATCAGAACAAATAAGCAGAGAAGAAATCGCTAAGACATTAGAATTAATTGAAAACCACTCAGTTTGA", "aa": "MEQNSSNANTNKSLIICNNYDKKLIKYFRYIVEMYIKTGEPVGSKRLVEKFKLSCSSATIRNAMAELEKLGLLEKKHVSGGRIPSSKGLEYYTKNLLYNAKKYFNEKLEDLLSKRRLSINTTLEEAANVVSQMAGFAVVATSNNESELLKSIQLTPLDEESAVVVIVTSSGNVQNKMFKFESGIELNDLRIAIRLFKERLIDTPLINLAKKTESLAPILSTQVKHFELIMQKFIKNIFCFEEKLENKSFNKNVIILSEQISREEIAKTLELIENHSV", "aa_hexdigest": "43d9cde88eba5c18e7a2e99669cbf04c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 586392, "stop": 587444, "observations": { "mutation": [ 586613 ], "stop": [ 586613 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8J7", "query_cov": 1.0, "subject_cov": 0.7914285714285715, "identity": 0.993, "score": 517.0, "evalue": 8.46e-184, "gene_start": 1, "gene_end": 277, "reference_start": 1, "reference_end": 277, "reference_sequence": "MEQNSSDTNTNKSLIICNNYDKKLIKYFRYIVEMYIKTGEPVGSKRLVEKFKLSCSSATIRNAMAELEKLGLLEKKHVSGGRIPSSKGLEYYTKNLLYNAKKYFNEKLEDLLSKRRLSINTTLEEAANVVSQMAGFAVVATSNNESELLKSIQLTPLDEESAVVVIVTSSGNVQNKMFKFESGIELNDLRIAIRLFKERLIDTPLINLAKKTESLAPILSTQVKHFELIMQKFIKNIFCFEEKLENKSFNKNVIILSEQISREEIAKTLELIENHSVWEAIENNLDEEKNIKLDFSHPNLNIISKRIQFINHKNIKEISILGPKNLDVEESLEAINLFEGIIKKEEEKGE" }, "paralog": false, "identity": 0.9914285714285714, "score": 665.0, "evalue": 1.51e-244, "description": "Internal stop codon at 586613. Nonsense mutation around 586613.", "psc": { "uniref90_id": "UniRef90_D1J8J7", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_B3PN01", "cog_id": "COG1420", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8J7", "UniRef:UniRef50_B3PN01", "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN01" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "DFOKDJOJIG_1032", "locus": "GCA013367855_01029" }, { "type": "tRNA", "sequence": "contig_1", "start": 587571, "stop": 587647, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(acg)", "amino_acid": "Arg", "anti_codon": "acg", "score": 76.1, "nt": "GCGCCCGTAGCTCAATTGGACAGAGTGTTTGGTTACGGCCCAAAAGGTTGCGGGTTCGACTCCTGCCGGGCGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 587611, 587613 ], "id": "DFOKDJOJIG_25", "locus": "GCA013367855_01030" }, { "type": "tRNA", "sequence": "contig_1", "start": 587650, "stop": 587724, "strand": "-", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 67.3, "nt": "GCCACCATAGCCAAACGGCCAAGGCATAGGTCTGCAACACCTTGATTACCGGTTCGAATCCGGTTGGTGGCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 587699, 587701 ], "id": "DFOKDJOJIG_24", "locus": "GCA013367855_01031" }, { "type": "cds", "sequence": "contig_1", "start": 587925, "stop": 588101, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCGAACGTGAACAAAAAAATAAAATTACTTTTATGAGCTCGCCTATTGATTTTACTTCTTCAAAACAACATATAATATTTAATAAAATGCCTGGTAATGATGGTATAACTAGGATTGAATCATTGCTTAAATTGACAGTCTTAAGATTGATGAAAAAGTTCATTTCGTTCTAA", "aa": "MSEREQKNKITFMSSPIDFTSSKQHIIFNKMPGNDGITRIESLLKLTVLRLMKKFISF", "aa_hexdigest": "0cf809e788642deac2151fcdf9a8dd95", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6776.965699999999, "isoelectric_point": 10.11536350250244 }, "id": "DFOKDJOJIG_1033", "locus": "GCA013367855_01032" }, { "type": "cds", "sequence": "contig_1", "start": 588126, "stop": 588446, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATACAAACCAATCATTGGAAAAATTTGCGCCAGAACTAAAAAATGAAATCAAAGATATTACTTCAATGAAATCAAATCAAGGATTATTGCTCATTGATCAAAATCTCGTTAATGCAAGAAATATTTTAAAATTTTTAGCTTTCTTATTTGGCACTAAGAAAAACAAAAGTGTAGCATTTGTTGACAACAATAATTTGTTTGAAGTCATGTCTAATAATTTAAAAAACTCATCAGAAATTAATTCAATTGTTCAAAATTTGATTTCTGTTGATTATTTATTCATAGATCGCTTTGCGATCGGAGTAAAACCGGAATGA", "aa": "MNTNQSLEKFAPELKNEIKDITSMKSNQGLLLIDQNLVNARNILKFLAFLFGTKKNKSVAFVDNNNLFEVMSNNLKNSSEINSIVQNLISVDYLFIDRFAIGVKPE", "aa_hexdigest": "183b3c99128504fb27e3f916375697ee", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12025.706099999989, "isoelectric_point": 7.965213966369628 }, "id": "DFOKDJOJIG_1034", "locus": "GCA013367855_01033" }, { "type": "cds", "sequence": "contig_1", "start": 588618, "stop": 588827, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAAAATATTCGATGAATTATTTAAAAATTCTCTTTTGAAAAATATTGATTTAGAGGCAGAAAAAGCAAAAATTTTAGCCGATCCAAATGTTAAAGCAATTATTGAAAAACTGAATTTAAATTCTAAACAAATCCAAAAAGGAATGAATTATTTGCAAAAATATTATAGCTATATTGAAATACATAAACAACTCCCTACATGA", "aa": "MENKIFDELFKNSLLKNIDLEAEKAKILADPNVKAIIEKLNLNSKQIQKGMNYLQKYYSYIEIHKQLPT", "aa_hexdigest": "613fbb912d524041840868c92ac55fd0", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8126.4028, "isoelectric_point": 8.860873222351074 }, "id": "DFOKDJOJIG_1035", "locus": "GCA013367855_01034" }, { "type": "cds", "sequence": "contig_1", "start": 588856, "stop": 589752, "strand": "-", "frame": 1, "gene": "dnaB2", "product": "DnaD domain-containing protein", "db_xrefs": [ "COG:COG3611", "COG:L", "KEGG:K03346", "SO:0001217", "UniRef:UniRef50_D1J8J9", "UniRef:UniRef90_D1J8J9" ], "nt": "ATGGTTGAAAATTTTTTTATTGAAAGCGAGAGGGAAATTACACAAAACGATTTTAAAAATCTAGTAATTTTTTATGCTCCGATTTTAGGCCATAGTAGCATATTTCTATATCAATTTTTATATAATTTAAAAAATATTTATACAAAAAATAAACTTTTTAATTTTTCTGAAATTAAAGATATTTTATTATTAAATGAACAAGAATTTGAAGAATGTCGTAGTAAACTAGAAGCGCTAAGCTTACTAAAAAGTTATTTAAACAAGGATGGTGTTTATATTTTTAGTTTAAATGCTCCATTGAATGCAAATGAAATAAGCAATAATAAAATTATTTCTAATATGTTAATTAAATTAATCACTAAATCAAAATATGAAGAATTATTAAAATTAAATGCAAATTATCAATTTGATAAAACAACAATTAATGATGTTTCTAAAAAATTTTATGAAGTATTTAATCTAGAAAATATAATTGATTTAAATACAAAAAATGAAGAATTGAATTTTAATTCAATTACTCTAGATGAATTAAAGGATAAGATAGAACCCGAACAATTTATTAGTCAATATACAAAAACAAATTTAAGCTTTAGCCAAAATGAAATGATTAGAAAATTAAAAAGAATGAAATTTAATAATTTTTCAATAAATGCTTTCATTTATTATTCAATTAAAATAAATCATTCAGTTGTATGCAAATATATTGAAAAAATAGCAGAAGATTTTAGTCATAGAAATTTATATGATGCCGAAAGAATTGATCAAGAATTAGAAAATGTTTATTTAATAAAACAAAATGCAAATAAAGAGTTAAAAAATATCAAAAAAAATGAAGATGAATCGGTTACTAGAGAAGATTTAAATTGTTTTTTAGATCTTGCAAATGAACATGATTGA", "aa": "MVENFFIESEREITQNDFKNLVIFYAPILGHSSIFLYQFLYNLKNIYTKNKLFNFSEIKDILLLNEQEFEECRSKLEALSLLKSYLNKDGVYIFSLNAPLNANEISNNKIISNMLIKLITKSKYEELLKLNANYQFDKTTINDVSKKFYEVFNLENIIDLNTKNEELNFNSITLDELKDKIEPEQFISQYTKTNLSFSQNEMIRKLKRMKFNNFSINAFIYYSIKINHSVVCKYIEKIAEDFSHRNLYDAERIDQELENVYLIKQNANKELKNIKKNEDESVTREDLNCFLDLANEHD", "aa_hexdigest": "e403e3b11aa94cb12bd26153cc458dd5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8J9", "query_cov": 1.0, "subject_cov": 0.9900332225913622, "identity": 0.987, "score": 565.0, "evalue": 4.2e-203, "valid": true, "gene": "DnaB2", "product": "DnaD domain-containing protein", "uniref50_id": "UniRef50_D1J8J9", "cog_id": "COG3611", "cog_category": "L", "kegg_orthology_id": "K03346" }, "pscc": { "uniref50_id": "UniRef50_D1J8J9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J9" ], "product": "DnaD domain-containing protein" }, "genes": [ "dnaB2", "dnaD" ], "id": "DFOKDJOJIG_1036", "locus": "GCA013367855_01035" }, { "type": "cds", "sequence": "contig_1", "start": 590064, "stop": 590855, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAATTAACCTTCACTTAAATACTATATTTTCTTTTTTAGAAAGTACAATAACCATTAATGATTTTATTCAAGAATTACTAAATAATAATATAGAGTATTTTGGTATTACAGAACATTGCAATTTTTATAGCATGCCACATTTTTTAAATGAAGCTAGAAAACATGATTTAAAGCCTATTTTTGGTCTTGACGTAAATGTAAAAATAGAAAATAATTTGTATAGATTTATAGTTTACTGCCAAAATAAAGAATCTTTTATGAACCTAAAAATGCTTTCTTTAAACTATGGAAAAAAAGGCTATATAGATTTAGCTGAAATTAGAGATTATTCTAATTTAATATGGATAGAACATCCAATTTTTGGCTATTATAAAAAAACTAATAATATTTTAGAAATTCAAAACCATTATTTTGCAATAAGCCAATATGATATAGACAATAGTGAAATACAAAATATTTTTGATAAGTGTTTATTAATTAATCACAATTCAATATTAAAATATGATGATAATTCAATAATTAAAACAATGTCAAATATTTCAAATAATAAAATATTTGAAAGCATTTACGAAGAATTTAAAATTGATATAGAAACAAAAAACAATTCAAAATTAGAAGAATTAATTGAAAGAACAAATAATTTTGCTAAAAAACTTTATTTTGAAATTGAAACTAAAGGTTTCAAAATGCCCAAATTCAAAAACGACAAAAATATTGCTTCTAATGAATATTTAAAATTGTTATTATTTAAATCAATAAGAGAAAAATTTGATAAATATTCATGA", "aa": "MKLINLHLNTIFSFLESTITINDFIQELLNNNIEYFGITEHCNFYSMPHFLNEARKHDLKPIFGLDVNVKIENNLYRFIVYCQNKESFMNLKMLSLNYGKKGYIDLAEIRDYSNLIWIEHPIFGYYKKTNNILEIQNHYFAISQYDIDNSEIQNIFDKCLLINHNSILKYDDNSIIKTMSNISNNKIFESIYEEFKIDIETKNNSKLEELIERTNNFAKKLYFEIETKGFKMPKFKNDKNIASNEYLKLLLFKSIREKFDKYS", "aa_hexdigest": "5111fb393dd89df22ccb09f17fea0a6c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31490.73740000004, "isoelectric_point": 6.4028215408325195 }, "id": "DFOKDJOJIG_1037", "locus": "GCA013367855_01036" }, { "type": "cds", "sequence": "contig_1", "start": 591099, "stop": 592301, "strand": "+", "frame": 3, "gene": "dnaE", "product": "DNA-directed DNA polymerase", "db_xrefs": [ "COG:COG0587", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J8K0", "UniRef:UniRef90_D1J8K0" ], "nt": "ATGCCTGACATTGATATTGATGTTCAAGATAATAAAAGAAATGATGTTATTGAATATTTATTAAAAAAATATGGGCATGAATTTGTGGCAAATATTGTTACTTTTTCTTCTCTAGGTAAAAAATCAGCGATTAGAGATATTTTAAGAATAAACAATATTGCCCCAACATTAATTAATGAAATATCAAAAAATATTGAAAATGATGAACAAGACCTATTAGTTGAATACAATGAAAATAGAAATTTACAATTATCATTAGCAAAATTAAATCCTAATGATGAATCATTTAGCACAAGAATATTAAACCAAGCAGCAAGGATATCAGGATTTTATAGACAAATTGGCACGCATGCTGCCGGATTAGTAATTAGCGACCAACCCGTCATCTCAATAGCACCAGTTCAATATAATTCTGAAAATTTTCTTCAAACTCAAATAAGTATGGAATATTTAGAAGATTTCGGGTTAGTTAAAATGGACATACTTGGTTTGAAAACATTGACGACTATTTCAAATATAGTTGATCTAATTTATCAAAATCAAAGTCTTAAAATTAACTTAAAAGATATAGACTTAACCGATCAAAAAACATTAGAATTATTGAGCTCTGGCCACACAAGAGGCGTTTTCCAAGTTGAAAGTCCAATCATGATAAAAGCCTTAAGACAGGTAAAGGTAAATAATTTTGAAGATATTGTTGCAATAATTTCATTAAATAGACCAGGTCCAATGAAAAACGTTTCGGTTTATGCAAAAAGAAAATTTGGACTAGAGTCGATACCAAAAATAAATAATGAATATGATAAAATTCTTGATTCTACTTATGGAATCATTGTATATCAAGAACAAATTATGAAAATTGCTCAAGTTGTTGCTAAAATGACATTCATTGAAGCTGACAATCTAAGAAGAATTATCTCTAAGAAAAAAATAAATGAAATGGAAGATATTAAATCTATTTTTATTGAAAGAGCAATGAAGAATGGCTATGAAAAATCTTTGGCTACAAATATCTTTAACAGCATAGAAAAATTTGCATCATATGGATTCAATAGATCTCATGCGGTTTCATATTCAATTATAACTTTTCAAATGGCCTTTTTAAAAGCTCATTACCCCTTAGAATTTTATACGTCATGTATAAGCTCGGCACACGGCTCTCAAGATACAATTGCAAAGTTCGTTAACGAGGCAAGTGAGTGA", "aa": "MPDIDIDVQDNKRNDVIEYLLKKYGHEFVANIVTFSSLGKKSAIRDILRINNIAPTLINEISKNIENDEQDLLVEYNENRNLQLSLAKLNPNDESFSTRILNQAARISGFYRQIGTHAAGLVISDQPVISIAPVQYNSENFLQTQISMEYLEDFGLVKMDILGLKTLTTISNIVDLIYQNQSLKINLKDIDLTDQKTLELLSSGHTRGVFQVESPIMIKALRQVKVNNFEDIVAIISLNRPGPMKNVSVYAKRKFGLESIPKINNEYDKILDSTYGIIVYQEQIMKIAQVVAKMTFIEADNLRRIISKKKINEMEDIKSIFIERAMKNGYEKSLATNIFNSIEKFASYGFNRSHAVSYSIITFQMAFLKAHYPLEFYTSCISSAHGSQDTIAKFVNEASE", "aa_hexdigest": "b6fcdeda05a5a749d6f2ce6a125eb6be", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "dnaE" ], "truncated": "both", "pseudogene": { "start": 590799, "stop": 592604, "observations": { "mutation": [ 590853, 590943, 590952, 592299 ], "stop": [ 590853, 590943, 590952, 592299 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K0", "query_cov": 1.0, "subject_cov": 0.40160642570281124, "identity": 1.0, "score": 758.0, "evalue": 7.83e-267, "gene_start": 1, "gene_end": 400, "reference_start": 346, "reference_end": 745, "reference_sequence": "MKLINLHLNTIFSFLESTITINDFIQELLNNNIEYFGITEHCNFYSMPYFLNEARKHDLKPIFGLDVNVKIENNLYRFIVYCQNKESFMNLKMLSLNFGKKGYIDLAEIRDYSNLIWIEHPIFGYYKKTNNILEIQNHYFAISQYDIDNSEIQNIFDKCLLINHNSILKYDDNSIIKTMSNISNNKIFESIYEEFKIDIETKNNSKLEELIERTNNFAKKLYFEIETKGFKMPKFKNDKNIASNEYLKLLLFKSIREKFDKYSWTDEYQQRFLKELKVIEDLNFADYFLVVQDWVNWAKNNNIKIGPGRGSAAGSLIGYLLNITEVDPIKYDLIFERFLNSNRVTMPDIDIDVQDNKRNDVIEYLLKKYGHEFVANIVTFSSLGKKSAIRDILRINNIAPTLINEISKNIENDEQDLLVEYNENRNLQLSLAKLNPNDESFSTRILNQAARISGFYRQIGTHAAGLVISDQPVISIAPVQYNSENFLQTQISMEYLEDFGLVKMDILGLKTLTTISNIVDLIYQNQSLKINLKDIDLTDQKTLELLSSGHTRGVFQVESPIMIKALRQVKVNNFEDIVAIISLNRPGPMKNVSVYAKRKFGLESIPKINNEYDKILDSTYGIIVYQEQIMKIAQVVAKMTFIEADNLRRIISKKKINEMEDIKSIFIERAMKNGYEKSLATNIFNSIEKFASYGFNRSHAVSYSIITFQMAFLKAHYPLEFYTSCISSAHGSQDTIAKFVNEASECGVEIVSPDINLSESEAIIKNRKIILPLTLIKGLGPEIANSIVENRSKYGPYKNFLDFLLKISSSQSIGKSTIELLIKANTLRSFNYNQNTLLNEIQKNNTDTMLFIKMFKNNIEGVSPNIINSYKPSEILNQDIMFEKKNEIELLGQVYNFNSSASNGMPGQTSFIDMHVGNEYLAIVNCTNIKQKISKKGNNYFELDLEANMQKIRAFYFTSNQEILNVKGKTIKVTLEKRNENIFYLRSIKEVLNEER" }, "paralog": false, "identity": 0.9916805324459235, "score": 1145.0, "evalue": 0.0, "description": "Internal stop codon at 590853, 590943, 590952, 592299. Nonsense mutation around 590853, 590943, 590952, 592299.", "psc": { "uniref90_id": "UniRef90_D1J8K0", "gene": "DnaE", "product": "DNA-directed DNA polymerase", "uniref50_id": "UniRef50_D1J8K0", "cog_id": "COG0587", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K0", "UniRef:UniRef50_D1J8K0", "COG:COG0587", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8K0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8K0" ], "product": "DNA-directed DNA polymerase" } }, "id": "DFOKDJOJIG_1038", "locus": "GCA013367855_01037" }, { "type": "cds", "sequence": "contig_1", "start": 592374, "stop": 593054, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14579.12" ], "nt": "TTGCCACTTACACTAATTAAGGGTCTTGGTCCCGAAATTGCTAATTCAATTGTTGAAAACAGATCAAAATATGGCCCATATAAAAATTTCCTAGATTTCTTATTAAAAATTTCTAGCTCTCAATCAATTGGCAAAAGTACAATTGAATTATTAATAAAGGCAAACTCTCTTAGATCGTTTAATTATAATCAAAACACCTTGTTGAATGAGATTCAAAAAAACAATACTGATACAATGTTATTTATAAAAATGTTTAAGAATAACATTGAAGGTGTTAACCCTAATATAATAAACTCTTATAAACCAAGTGAAATTATTAATCAAGATATCATGTTTGAAAAGAAAAACGAAATTGAATTATTAGGTCAAGTTTACAATTTTAATTCAAGCGCAAGCAATGGAATGCCTGGACAAACTAGTTTTATAGACATGCACGTTGGAAATGAATATTTAGCAATTGTTAACTGTACAAACGTAAAGCAAAAAATTTCTAAAAAAGGTAATAACTATTTTGAATTAGATTTAGAAGCAAACATGCAAAAAATAAGAGCATTTTATTTTACATCAAATCAAGAAATACTAAATGTTAAGGGAAAAACTATCAAAGTAACTCTAGAAAAAAGAAATGAAAATATTTTTTATTTGCGTTCAATAAAGGAAGTTTTAAATGAAGAAAGATAA", "aa": "MPLTLIKGLGPEIANSIVENRSKYGPYKNFLDFLLKISSSQSIGKSTIELLIKANSLRSFNYNQNTLLNEIQKNNTDTMLFIKMFKNNIEGVNPNIINSYKPSEIINQDIMFEKKNEIELLGQVYNFNSSASNGMPGQTSFIDMHVGNEYLAIVNCTNVKQKISKKGNNYFELDLEANMQKIRAFYFTSNQEILNVKGKTIKVTLEKRNENIFYLRSIKEVLNEER", "aa_hexdigest": "70e960cf4de56d74e8e6947229b8ac60", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14579.12", "name": "HHH_6", "length": 65, "aa_cov": 0.28761061946902655, "hmm_cov": 0.9846153846153847, "evalue": 1.474079266658582e-14, "score": 49.685672760009766, "start": 2, "stop": 66 } ], "seq_stats": { "molecular_weight": 25957.47540000002, "isoelectric_point": 9.097149848937988 }, "id": "DFOKDJOJIG_1039", "locus": "GCA013367855_01038" }, { "type": "cds", "sequence": "contig_1", "start": 593041, "stop": 593934, "strand": "+", "frame": 1, "gene": "exoIX", "product": "DNA polymerase I", "db_xrefs": [ "COG:COG0258", "COG:L", "RefSeq:WP_020002546.1", "SO:0001217", "UniParc:UPI0003651CA3", "UniRef:UniRef100_A0A2K9YTX0", "UniRef:UniRef50_A0A318U4A1", "UniRef:UniRef90_D1J8K1" ], "nt": "ATGAAGAAAGATAATGAACAACTGTTGATTATAGATGGTACATTTTTAGCATACAGATCCTATTATGCAACATCTTATAACGCAACTCAAATTTTAACCACATCAGAAGGCTTTGAAACCAATGCGATAGTAGCATTCTTTAAAACACTTTTTTTATTACTAAAAAAATTAAATCCTAAATATATCTATATAGCCTTTGATTCGCATGTTAAGACGTTTAGGCATGAATTATTTCCTTCTTATAAAGATGGTAGAAAAAAAGCGCCAGAAAGTTTTTATCTACAGCTTGATTTAATTCAAGAAATATTAACAAATATTAATATTCAAAACCAATATTATAATGGCTTTGAAGCTGATGATATAATTGCAAAAGTTAAAAATAAATTTAATTTGAATACAATCATTTTTTCAGGAGATCAAGATTTAAATCAATTAATAGATGAACAAACAGCAATCATTAAAAAAGATAAAACTGGTTCTTTCTTTTTTCTTAATTCAAAGAATTTTTCAGAATATTATGATTTTTTACCAAATCAAGTTATTGATTATAAAGCATTAGTCGGTGATAACAGTGATAATTTTAAGGGTGTTGTAGGAATTGGTCCTAAATCTGCTAAAACTTTATTAGAAGATTATAAGACTATTGAAAATATATACAATAATATTGAAAAAATAAAACCTTCAATTGCCAAAAAATTAATGGAATATAAAGAAAATACATTTCTCGATAAATATTTAGCAACCTTAAGGATAGACTTTGATTTGCAACTTCCAGAATTAGAAAATTTAGCAATATCTAATATTGCAATTTCAAACAGTGCAAAACAAATTATTGACAAATTTGAACTATATTCTTTGGAAAATTCTTTGAAGAATTTAATCAATAAAAAATAA", "aa": "MKKDNEQLLIIDGTFLAYRSYYATSYNATQILTTSEGFETNAIVAFFKTLFLLLKKLNPKYIYIAFDSHVKTFRHELFPSYKDGRKKAPESFYLQLDLIQEILTNINIQNQYYNGFEADDIIAKVKNKFNLNTIIFSGDQDLNQLIDEQTAIIKKDKTGSFFFLNSKNFSEYYDFLPNQVIDYKALVGDNSDNFKGVVGIGPKSAKTLLEDYKTIENIYNNIEKIKPSIAKKLMEYKENTFLDKYLATLRIDFDLQLPELENLAISNIAISNSAKQIIDKFELYSLENSLKNLINKK", "aa_hexdigest": "a6b2523fe6605cc4d24d3bfc903ae80d", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0003651CA3", "ncbi_nrp_id": "WP_020002546.1", "uniref100_id": "UniRef100_A0A2K9YTX0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003651CA3", "RefSeq:WP_020002546.1", "UniRef:UniRef100_A0A2K9YTX0" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTX0", "uniref90_id": "UniRef90_D1J8K1" }, "psc": { "uniref90_id": "UniRef90_D1J8K1", "gene": "ExoIX", "product": "DNA polymerase I", "uniref50_id": "UniRef50_A0A318U4A1", "cog_id": "COG0258", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_A0A318U4A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318U4A1" ], "product": "5'-3' exonuclease, exo domain of DNA polymerase I" }, "genes": [ "exoIX" ], "id": "DFOKDJOJIG_1040", "locus": "GCA013367855_01039" }, { "type": "cds", "sequence": "contig_1", "start": 594075, "stop": 594449, "strand": "+", "frame": 3, "gene": null, "product": "Helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "nt": "ATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGTTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCGAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAGTTTTCCAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGA", "aa": "MNYNIHKYFHLSLEERFFIQRSLENNLSLRAISRQLNRSASTISREIKRNSNFNEYIFLRADKKSKFRAYHKYMFKFNINFEFEEFSKIFKSKYDKKYFGVKATMNYIEENFNVKKPTMRTVFN", "aa_hexdigest": "c57bd1123260a439ee4f69e485682c4a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0AAJ1UWR4", "query_cov": 1.0080645161290323, "subject_cov": 0.8116883116883117, "identity": 0.6, "score": 141.0, "evalue": 1.13e-40, "valid": false, "product": "Helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_A0AAJ1UWR4" }, "pscc": { "uniref50_id": "UniRef50_A0AAJ1UWR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "product": "Helix-turn-helix domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_1041", "locus": "GCA013367855_01040" }, { "type": "cds", "sequence": "contig_1", "start": 594633, "stop": 594914, "strand": "+", "frame": 3, "gene": null, "product": "IS30 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "nt": "TTGGTTCTTGGAAAAAGAGCAAATGGCTATAAAAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGCGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGA", "aa": "MVLGKRANGYKNILTLTERKTRVGFATFVSSKSPFEINSKIKELIKNKNLIVKTITIDNGIEFEKIGFLAKSLNIIIYRAEPYASFQRGSNEN", "aa_hexdigest": "151ece11318bfe9f9904904a28af0e1a", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001F20DEBE", "query_cov": 0.978494623655914, "subject_cov": 0.8272727272727273, "identity": 0.67, "score": 125.0, "evalue": 1.07e-35, "valid": false, "product": "IS30 family transposase", "uniref50_id": "UniRef50_UPI001F20DEBE", "go_ids": [ "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F20DEBE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "product": "IS30 family transposase" }, "genes": [], "id": "DFOKDJOJIG_1042", "locus": "GCA013367855_01041" }, { "type": "cds", "sequence": "contig_1", "start": 594921, "stop": 595031, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAGAAGAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGA", "aa": "MIRREYQKGFNFNILTQEDLDTTIAKINNMPREILN", "aa_hexdigest": "8ef7b9776e982e4522979ce89f78742f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4340.9349, "isoelectric_point": 6.038881492614746 }, "id": "DFOKDJOJIG_1043", "locus": "GCA013367855_01042" }, { "type": "cds", "sequence": "contig_1", "start": 595259, "stop": 595630, "strand": "-", "frame": 3, "gene": "cof", "product": "HAD family phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_A0A318UA29", "UniRef:UniRef90_D1J8K2" ], "nt": "TTGCCAGCAACAACAAAAATTTTAACTTTTGGGAAGTCAAAAAAAGGAATAATAAAACTTAGAGATGAATTATTAAATGCTTTTCATGATATTTCAACACATATTGTATCAAAAGGATATTCAATTGAAATTAGCGATATTAATGCTAATAAAGGAATTGGCGATCGATTTGTATGTGATTTGTTAAATATTGATTATAAAGAAGCAGTTCACTTAGGCGATTCGGGCAATGATGTTGTTACTCAAAAATATTTAGGTGGCTTTGTAGCGATGAAAAATTCTACAAAGAACATAAAACAAAAAGCAGATTATGTAGGATATTCATATAAAAAATCTGGAGTAGCAAAAACATTGCAAGAACTTCAAAAATAA", "aa": "MPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTQKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK", "aa_hexdigest": "e2821e4ba0226feb7075948f6be44d6e", "start_type": "TTG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 595259, "stop": 595930, "observations": { "mutation": [ 595675 ], "stop": [ 595675 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K2", "query_cov": 1.0, "subject_cov": 0.458955223880597, "identity": 0.9840000000000001, "score": 240.0, "evalue": 3.49e-78, "gene_start": 1, "gene_end": 123, "reference_start": 146, "reference_end": 268, "reference_sequence": "MINFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARAWLKKLNKVTYDEIQSLPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTKKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK" }, "paralog": false, "identity": 0.9910313901345291, "score": 435.0, "evalue": 1.48e-157, "description": "Internal stop codon at 595675. Nonsense mutation around 595675.", "psc": { "uniref90_id": "UniRef90_D1J8K2", "gene": "Cof", "product": "HAD family phosphatase", "uniref50_id": "UniRef50_A0A318UA29", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K2", "UniRef:UniRef50_A0A318UA29", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318UA29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318UA29" ], "product": "COF family HAD hydrolase protein" } }, "id": "DFOKDJOJIG_1044", "locus": "GCA013367855_01043" }, { "type": "cds", "sequence": "contig_1", "start": 595673, "stop": 596065, "strand": "-", "frame": 3, "gene": "cof", "product": "HAD family phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_A0A318UA29", "UniRef:UniRef90_D1J8K2" ], "nt": "ATGATTGATTTTAAACCTAAGGCATATTTTTTGGATATGGATGGCACATATCTAGATTTGCCTCGCAAAAAACAAATTATGATAAGCGAAAAAAATGTTGAGGTTGCTAAGGACTTCAATAATAGAGGAATCCCTGTTATTTTGTCAACAGGTAGAGGAAATTCAAAGTTTGTTTTGGATTTAACTAATAAAATAAATGCACCGTTTGCTGTTTGCCAAAATGGTGGAGTAATTGTAGACAAAAATAATAATGTTTTGAAACGCCATGAAATTAACCCTGATACATTGAAATCAATAATTGAAATTTTAATTGCACAAAAAATGTTTTTTATTTTTAATAGTGGCGATACAATATATGGAACAAAAACAAAATTTAGAATCGCTAGAGCTTGA", "aa": "MIDFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARA", "aa_hexdigest": "a1866e85facaa78a9fbe437420c7ae80", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "cof" ], "truncated": "5-prime", "pseudogene": { "start": 595370, "stop": 596065, "observations": { "mutation": [ 595675 ], "stop": [ 595675 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K2", "query_cov": 1.0, "subject_cov": 0.48507462686567165, "identity": 0.992, "score": 260.0, "evalue": 5.83e-86, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MINFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARAWLKKLNKVTYDEIQSLPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTKKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK" }, "paralog": false, "identity": 0.987012987012987, "score": 452.0, "evalue": 2.74e-164, "description": "Internal stop codon at 595675. Nonsense mutation around 595675.", "psc": { "uniref90_id": "UniRef90_D1J8K2", "gene": "Cof", "product": "HAD family phosphatase", "uniref50_id": "UniRef50_A0A318UA29", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K2", "UniRef:UniRef50_A0A318UA29", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318UA29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318UA29" ], "product": "COF family HAD hydrolase protein" } }, "id": "DFOKDJOJIG_1045", "locus": "GCA013367855_01044" }, { "type": "cds", "sequence": "contig_1", "start": 596073, "stop": 596279, "strand": "-", "frame": 2, "gene": null, "product": "Thioredoxin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P2T3" ], "nt": "ATGATTGGCCCAGAATTAGAAAAATTAGCAGAAGAAAATTCAGATGTTAATGTTTTTGATTTGAACGTAGATAAATTCATTGATTTAGCAAGAGAAATGAATGTATCTCATATTCCATCGTTATTTTATTATAGAGACGGTAAGTTAGTTTCTCAAAGTGTAGGATACTTGCCAGTTGAAGAAATTTTAAACAATTTAAATAAGTAG", "aa": "MIGPELEKLAEENSDVNVFDLNVDKFIDLAREMNVSHIPSLFYYRDGKLVSQSVGYLPVEEILNNLNK", "aa_hexdigest": "d6a9e1f63bb324bb8081203c85a066ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P2T3", "query_cov": 0.8382352941176471, "subject_cov": 0.9193548387096774, "identity": 0.526, "score": 72.8, "evalue": 1.09e-15, "valid": false, "product": "Thioredoxin", "uniref50_id": "UniRef50_A0A3B0P2T3" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P2T3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P2T3" ], "product": "Thioredoxin" }, "genes": [], "id": "DFOKDJOJIG_1046", "locus": "GCA013367855_01045" }, { "type": "cds", "sequence": "contig_1", "start": 596295, "stop": 596459, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATACTTGTTTTTGAACTAATAGAAAAACAAAAAGATTTGTATTATTTGCAAGAGCATATTAAAGGAGAAAATATTATGTATAAAACTATTGAATCAGTTGAAGAATTCAAAAAGAATGAATATTTAGAAGGAAAATCAATTGTTGCATTTAGAGCAGTTTGA", "aa": "MILVFELIEKQKDLYYLQEHIKGENIMYKTIESVEEFKKNEYLEGKSIVAFRAV", "aa_hexdigest": "e3c34a065d1b30a44f763fd2991c67c8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6499.5041, "isoelectric_point": 5.297132682800293 }, "id": "DFOKDJOJIG_1047", "locus": "GCA013367855_01046" }, { "type": "cds", "sequence": "contig_1", "start": 596443, "stop": 596565, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09180.16" ], "nt": "GTGTATGCTGCATTTGATGGTAACGGTGATGATGAAGTAAAAATTAAAGAAGAAACAGGAGCATCAACAAGGTGTATTCCATTTAAAGAACCAATAAAAGTTTCAAATGATACTTGTTTTTGA", "aa": "MYAAFDGNGDDEVKIKEETGASTRCIPFKEPIKVSNDTCF", "aa_hexdigest": "79c1090abc86c7c2c3a94b405e703492", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09180.16", "name": "ProRS-C_1", "length": 40, "aa_cov": 0.95, "hmm_cov": 1.0, "evalue": 7.372057168850756e-11, "score": 37.764034271240234, "start": 3, "stop": 40 } ], "seq_stats": { "molecular_weight": 4427.896500000001, "isoelectric_point": 4.507070732116699 }, "id": "DFOKDJOJIG_1048", "locus": "GCA013367855_01047" }, { "type": "cds", "sequence": "contig_1", "start": 596566, "stop": 597018, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGACTCATGGTGATGATCGAGGAATAATCATTCCACCAAAAATAGCAAGGACTCAAGTTGATATATTAGAAATTTTTAATTCAAAGGATGAACGAGTTCACAAGCTTGCCCTTGAATTAAAAGATGCATTATTAGCAAAAAATATTGTTGCAAACATAGATTCTTCAAACAAAGGTCCAGGTTTTAAAGCAGCAAATTCAGAAATTCATGGTACACCATTGAGAATAGAAATTGGTCCAAAGGATATTGAAAATAATAGTGTATTATTCGTTAGACGTGATAACCTAGAAAAAATTACTTCAACAATTGCTGAAGCAGCAGATATTGCCAGTCTCTTATTACAAAAAATACAAGACAATTTATTTGAAAGTGCTAAACAACGCTTACAAAATAATATTGTAACTGTTAATAATTATCAAGAATTTAAGGAAGCAATTGCTGCGGGTCATTGA", "aa": "MTHGDDRGIIIPPKIARTQVDILEIFNSKDERVHKLALELKDALLAKNIVANIDSSNKGPGFKAANSEIHGTPLRIEIGPKDIENNSVLFVRRDNLEKITSTIAEAADIASLLLQKIQDNLFESAKQRLQNNIVTVNNYQEFKEAIAAGH", "aa_hexdigest": "735cb7364e68034509e04919925d4a8c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 82, "aa_cov": 0.5466666666666666, "hmm_cov": 1.0, "evalue": 7.692442059993948e-16, "score": 53.748966217041016, "start": 27, "stop": 108 } ], "seq_stats": { "molecular_weight": 16620.72079999999, "isoelectric_point": 6.082818031311034 }, "id": "DFOKDJOJIG_1049", "locus": "GCA013367855_01048" }, { "type": "cds", "sequence": "contig_1", "start": 597052, "stop": 597297, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACAAGGTTTATAAAGATGTTATTGAAGAAAATTTAGCAATTCCAGTAGTAGAAGGCTGCAAAACGGATCATGAAAAATTTGCTGGCGCAGATATAACATACACCTTAGAATCAATGATGAAGGACGGAAAGGCACTTCAATCGGGTACTAGTCACTATTTGGGACAAAATTTTACTAAGGCATTCGATATAACATTCAAAAATAATGAAAATAAATTAATTAATCCTTTTGGTACCAGTTGA", "aa": "MHKVYKDVIEENLAIPVVEGCKTDHEKFAGADITYTLESMMKDGKALQSGTSHYLGQNFTKAFDITFKNNENKLINPFGTS", "aa_hexdigest": "9f9f35b3fc6881aaeb1e7cdccceaac90", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9072.162599999992, "isoelectric_point": 5.837330436706543 }, "id": "DFOKDJOJIG_1050", "locus": "GCA013367855_01049" }, { "type": "cds", "sequence": "contig_1", "start": 597418, "stop": 597669, "strand": "-", "frame": 1, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "nt": "ATGGATATTGAATTTAAAAAGAGAGGCGTTCAAAACGTTTATTTACCTCTTTTAATACCAGAAAGCTTATTACAAAAAGAAAAAAATCATGTCGAAGGATTTAATCCTGAACTTGCAACTGTTACTGAAGTAGGCGGCAAAAAATTGGCAGAAAAGTTTTTTATAAGACCAACTTCAGAAACATTGTTTGCTGATTTATTCAAAAATGAGGTTTCGTCTTACAATGATTTGCCAATAATTTATAATCAATGA", "aa": "MDIEFKKRGVQNVYLPLLIPESLLQKEKNHVEGFNPELATVTEVGGKKLAEKFFIRPTSETLFADLFKNEVSSYNDLPIIYNQ", "aa_hexdigest": "be7654839c9a3e753250bfb8135032dd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A661HMY4", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.614, "score": 102.0, "evalue": 4.5e-27, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A661HMY4" }, "pscc": { "uniref50_id": "UniRef50_A0A661HMY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_1051", "locus": "GCA013367855_01050" }, { "type": "cds", "sequence": "contig_1", "start": 597888, "stop": 598283, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGTTGCAATTGACATTGCTTCTTCAAATACCAAAAACCAATTTGTATTTGTAAACCTTGTTCAATTTGACAGCCATTTTGGACATAGAGAAGACCCAATAGGATATTCACAAAATATTTCAAGGCTAGATATTAAATTAACAAAATTGATAAACGCAATGAAATCAGATGACTTGTTAATTATAACTTCTGACCATGGAAATGACCCAACAAAAGGCCCTGATCATACAAGAGAAGCATTGCCATTGACAATATTTTCAAAGAAATTCAAATCACCATGTGTATTTAAAGAACCTTTGAAAGGCTTGGGAACTGCAGGAAATATTGTTGCAAGAAATTTCAATGTTCCAACCGTTCAAACAGGTGAAGATATTTTTGATAAATTAATCTAA", "aa": "MDVAIDIASSNTKNQFVFVNLVQFDSHFGHREDPIGYSQNISRLDIKLTKLINAMKSDDLLIITSDHGNDPTKGPDHTREALPLTIFSKKFKSPCVFKEPLKGLGTAGNIVARNFNVPTVQTGEDIFDKLI", "aa_hexdigest": "efe568986058dda60c7d493c057c6d40", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14543.415699999978, "isoelectric_point": 6.397819709777832 }, "id": "DFOKDJOJIG_1052", "locus": "GCA013367855_01051" }, { "type": "cds", "sequence": "contig_1", "start": 598503, "stop": 598805, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGAATTTATACAAAAGTTCCAAACCCACAATTCATTGAAAAAGGTTTCCCTGCTGATTTAGTCGCAGAATTATCAAAAGCGTTTGATAATAGAAAAATTATTGGAAATAGAAATGCATCAGGCACAGTTATATTGGCCGAATTAGGTTTGCAACACATTGAAAAAGGAGATATGATAATTTACACTTCTCCTGATTCGACATTGCAAATTATTGGGGATGAAAAAACATTGGGTGTTGAAAAACTAACTGAATATGCAAAGAGAGCAAGAGCAATTTGTTCATCAAGACCTGAATGA", "aa": "MMGIYTKVPNPQFIEKGFPADLVAELSKAFDNRKIIGNRNASGTVILAELGLQHIEKGDMIIYTSPDSTLQIIGDEKTLGVEKLTEYAKRARAICSSRPE", "aa_hexdigest": "5d61518090ed1c2deb07abf8c68a2cfe", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11024.595599999986, "isoelectric_point": 6.585217857360839 }, "id": "DFOKDJOJIG_1053", "locus": "GCA013367855_01052" }, { "type": "cds", "sequence": "contig_1", "start": 598935, "stop": 599084, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAACAAAAATTTAATAGAATATTTTTTATAGTTACAGATGGTCTTGGCATTGGATATGAACCACGCCAAGCTGAATTTCATGATGAAGGTGCAAATAGCATGTTGCATGCATCAGAAGCATTGCCACTAGAAATTCCTACATGA", "aa": "MQEQKFNRIFFIVTDGLGIGYEPRQAEFHDEGANSMLHASEALPLEIPT", "aa_hexdigest": "4eec1535153f3fde8ad1d1f95641cfd4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5549.166199999999, "isoelectric_point": 4.583291816711426 }, "id": "DFOKDJOJIG_1054", "locus": "GCA013367855_01053" }, { "type": "cds", "sequence": "contig_1", "start": 599376, "stop": 600626, "strand": "+", "frame": 3, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_L0RUB4", "UniRef:UniRef90_D1J8K6" ], "nt": "ATGAAATTTAATGAACTAAGAACTAAAATTGTTGCAACAATTGGCCCTTCTTCAGATAATTATGACACAATGAAGAGTTTAATTGAAGCCGGAGTTACTACAATTAGAGCCAATTTTTCACATGGCGGTCCTGAAACTCATGTTAATAAATTTGAAATAGCAAGACAAATTTCAAAAGAACTAAACATTCCAATCTCATTAATGCTTGACACAAAAGGCCCTGAAATTAGAGTTGGAAAAATGGAAAATGATGGAGTTACAATCCCTGCAAATCACATTTTAAAAATTAGAACAGATGAAAATTCATACAAGAATTTTTTAGGCACCCAAGATCAAATTACTGTTTCATATAGAATGGACAACGATCTTAAAATAGGGGATAAAGTTTTAATTGATGATGGAAAATTAGTTACAACTGTAAAGGATATTAAAAAATTTGAAATTACTGTTGAAACAGTTAATGAACATTTTTTAAAAACTAATAAAAGAATTAACATTCCTGGTGTTAACTTCTCACTTCCTTTTTTAAGTCAAAAGGATATTGACGATATTATATGGGGTGCAAAATACAAAGTTGATTATATTGCAGCATCATTTGTAAATTCTGCTAAAAATGTAAAAGAAATTAGAGACATATTAAATGCAAATGGCGGAGAAAACATTCAAATCATTTCAAAAATTGAATCTAAGATGGCAATTAATAACATTGATGAAATAATTGAAGCTTCAGACGGAATTATGGTAGCAAGAGGGGACCTTGGACTTGAAATTCCTTACTATGATGTTCCTTACTACGAAAAGAAAATAATTCGTAAATGTAGATTAGTCGGCAAGGAAGTTATAGTTGCAACTCAAATGTTAGATTCAATGGAAAACAATCCTATTCCAACAAGAGCGGAAGTTACCGATGTTTATTATGCAGTTGAATTAGGTGCAGACTCAACAATGTTATCCGGAGAATCTGCAAGTGGTAAATACCCTGTTGAAGCAGTTAAAGTTATGTCTAAAATTGCAAAAAGAGCAGAAAAAGAATATTACAATGAATTATTCTATGGCAAACAAATAGAAAAAGTAGCAAATCATCCTACTTCAAGAATGCTAATTGCTTATGAAGTTGCCAAAATCCTTCAAAGAGGTGAATACAAATTTGCCATTATATTAAGTCACACCGGAAAATTATTAAAAGAAGTTGCTAAATACAGACCAAACGCATTTATTGTTGGAGTTGTTAGCGACAATAGATTAGTTTGA", "aa": "MKFNELRTKIVATIGPSSDNYDTMKSLIEAGVTTIRANFSHGGPETHVNKFEIARQISKELNIPISLMLDTKGPEIRVGKMENDGVTIPANHILKIRTDENSYKNFLGTQDQITVSYRMDNDLKIGDKVLIDDGKLVTTVKDIKKFEITVETVNEHFLKTNKRINIPGVNFSLPFLSQKDIDDIIWGAKYKVDYIAASFVNSAKNVKEIRDILNANGGENIQIISKIESKMAINNIDEIIEASDGIMVARGDLGLEIPYYDVPYYEKKIIRKCRLVGKEVIVATQMLDSMENNPIPTRAEVTDVYYAVELGADSTMLSGESASGKYPVEAVKVMSKIAKRAEKEYYNELFYGKQIEKVANHPTSRMLIAYEVAKILQRGEYKFAIILSHTGKLLKEVAKYRPNAFIVGVVSDNRLV", "aa_hexdigest": "391d450413f92cf50f5da062789af7a0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8K6", "query_cov": 1.0, "subject_cov": 0.879492600422833, "identity": 0.993, "score": 807.0, "evalue": 5.99e-294, "valid": true, "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_L0RUB4", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_L0RUB4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RUB4" ], "product": "Pyruvate kinase" }, "genes": [ "pyk" ], "id": "DFOKDJOJIG_1055", "locus": "GCA013367855_01054" }, { "type": "cds", "sequence": "contig_1", "start": 600992, "stop": 601711, "strand": "-", "frame": 3, "gene": null, "product": "Restriction endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q098", "UniRef:UniRef50_UPI001E622C03", "UniRef:UniRef90_A0A6A8Q098", "UniRef:UniRef90_UPI001E622C03" ], "nt": "ATGTGTAAATTGGGTTTATATAATAGCAATTCAAAAACGATTTTAAAATCTGAATTGAATAATTTTTTTAATTTAGAAAATTTGAAATATTTAAATGAAGACAATTTTTATTCATTGCCAATTTTTAAGGTGGATAATATTGAAAAAAATATCAAGTTTCCATTTAAATATGAAGAAGTAATAGATATTTTAAATCAAATTCAAAGTGAAAAATTTGAATTTAAAGAAGCACCATCAGAAAAATACATTGCCCTAAAACACGTGCCAAATTTTGCGTTAGCAGAATATTTTGTTAATTTATTCTTCACATATTCCCTTAATATTAATCCGCAAAATTTTAAAAGTATATGTCATACAAAATTAACAAAGAATTTGTATCCAATTTTTTCTGCTCCCGGACATTCGGCAGATTTCGATTATTATGATTCAAAAAACAACATTCAGTATATAGTTGAAACGACTATACTAAAAACAGAGAAGGAAGTTGTTGATGGTGAAAATTATTCAATATCAAAACATGCACAAAACCTAGTTAACGCAAATCAAAATATAAAGGAAATTAATTTATATTTTGTAAATTTTTTAAAATTTAACGAAGAACAAAAAAAAGAATTAGAATCCGTATTTTTATCTACATATAAATCAATGCTTGAAAAAAATAATGTTGGGAAAAATATTTATATAAAAAAATTCTCTGAATTAATTAATTTAAATTTATAA", "aa": "MCKLGLYNSNSKTILKSELNNFFNLENLKYLNEDNFYSLPIFKVDNIEKNIKFPFKYEEVIDILNQIQSEKFEFKEAPSEKYIALKHVPNFALAEYFVNLFFTYSLNINPQNFKSICHTKLTKNLYPIFSAPGHSADFDYYDSKNNIQYIVETTILKTEKEVVDGENYSISKHAQNLVNANQNIKEINLYFVNFLKFNEEQKKELESVFLSTYKSMLEKNNVGKNIYIKKFSELINLNL", "aa_hexdigest": "f9087b7c503f52d6e2681de2618060fe", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E622C03", "query_cov": 0.8117154811715481, "subject_cov": 0.8899082568807339, "identity": 0.995, "score": 382.0, "evalue": 3.31e-133, "valid": true, "uniref50_id": "UniRef50_UPI001E622C03" }, "pscc": { "uniref50_id": "UniRef50_UPI001E622C03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E622C03" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 600992, "stop": 602011, "observations": { "mutation": [ 601714 ], "stop": [ 601714 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8Q098", "query_cov": 1.0, "subject_cov": 0.45610687022900764, "identity": 0.996, "score": 465.0, "evalue": 2.6e-161, "gene_start": 1, "gene_end": 239, "reference_start": 286, "reference_end": 524, "reference_sequence": "MKKQGFNKFGGTEFRVAEPINEYFSILSWVNYERISIENNLSNNLQKNFNVNWDEEFKKYVKNQKIYNYSNDKKIKSRFNFVISHLKKWGFLKNSKNLVFNFEPKKFFDTDSPVEELFFKKMYLYWEEFREMVNFIISCNESSISIEKLNWAFAVFDYKEDKFEDLYAKNKQQILEEIADVSNITNIKSLFKKKINEYKILEKIFNLLRIKEPLSYSDYLELKPKEFKYIKRLLNIPPKERNVNYLDKINFYIFNHLQEDFLLDLKKISIEFNIEKEYMDMFNRWMCKLGLYNSNSKTILKSELNNFFNLENLKYLNEANFYSLPIFKVDNIEKNIKFPFKYEEVIDILNQIQSEKFEFKEAPSEKYIALKHVPNFALAEYFVNLFFTYSLNINPQNFKSICHTKLTKNLYPIFSAPGHSADFDYYDSKNNIQYIVETTILKTEKEVVDGENYSISKHAQNLVNANQNIKEINLYFVNFLKFNEEQKKELESVFLSTYKSMLEKNNVGKNIYIKKFSELINLNL" }, "paralog": false, "identity": 0.9911504424778761, "score": 672.0, "evalue": 5.41e-245, "description": "Internal stop codon at 601714. Nonsense mutation around 601714.", "psc": { "uniref90_id": "UniRef90_A0A6A8Q098", "product": "Restriction endonuclease", "uniref50_id": "UniRef50_A0A6A8Q098", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8Q098", "UniRef:UniRef50_A0A6A8Q098" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q098", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q098" ], "product": "Restriction endonuclease" } }, "id": "DFOKDJOJIG_1056", "locus": "GCA013367855_01055" }, { "type": "cds", "sequence": "contig_1", "start": 601712, "stop": 601948, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAGAATCAAAGAACCTTTGTCATATAGTGATTATTTAGAATTGAAACCCAAAGAATTTAAATATATAAAAAGATTATTAAATATTCCCTCAAAAGAAAGAAATGTAAACTATTTAGATAAAATAAATTTTTACATATTTAATCATTTACAAGAAGATTTTTTGCTAGATTTAAAAAAAATCTCTATTGAATTTAATATCGAAAAAGAATATATGGACATGTTCAATAGATGA", "aa": "MLRIKEPLSYSDYLELKPKEFKYIKRLLNIPSKERNVNYLDKINFYIFNHLQEDFLLDLKKISIEFNIEKEYMDMFNR", "aa_hexdigest": "343e5c3a381dca3d6197aa5890ac3490", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9741.266999999996, "isoelectric_point": 7.901583671569825 }, "id": "DFOKDJOJIG_1057", "locus": "GCA013367855_01056" }, { "type": "cds", "sequence": "contig_1", "start": 603164, "stop": 603568, "strand": "-", "frame": 3, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BBF45A" ], "nt": "ATGGGTTCTGGTTCGACTTGCGTTGCGGCCTTAAAATTGCAAAGGAATTTTGTTGGATGTGAGATAGATAAAAAATATTTTTGTGCTGCAAAGCAAAGAATAGAAAGGTATGAGCTAGAAATGAATAAAATTAATAGATCACCATTGTATTATTTGGGCGATAAATATAAATTATTACCACAATTAAAAAAATTATTTCCAAATAATATTAGAAATTTTTATGATGCATTTGCCGGAGGAGGAACGGTTATTGCAAATGTTATAGCTCAAAAATATTTTTACAATGATAAGCAAAAAGAAGTGTGCAATATTGTAAAACTTCTCTCAAATACTAACCCTATAAAAATTTTAAATGATATATATATATATATATATATAAGAAGTATGGCTCAATTGATAAATTAG", "aa": "MGSGSTCVAALKLQRNFVGCEIDKKYFCAAKQRIERYELEMNKINRSPLYYLGDKYKLLPQLKKLFPNNIRNFYDAFAGGGTVIANVIAQKYFYNDKQKEVCNIVKLLSNTNPIKILNDIYIYIYIRSMAQLIN", "aa_hexdigest": "936cfc772d97b4ac3f869bd803c67650", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0035BBF45A", "query_cov": 0.9328358208955224, "subject_cov": 0.8620689655172413, "identity": 0.56, "score": 137.0, "evalue": 2.93e-39, "valid": false, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0035BBF45A" }, "pscc": { "uniref50_id": "UniRef50_UPI0035BBF45A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BBF45A" ], "product": "DNA methyltransferase" }, "genes": [], "id": "DFOKDJOJIG_1058", "locus": "GCA013367855_01057" }, { "type": "cds", "sequence": "contig_1", "start": 604379, "stop": 604507, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTTCAGGCTTAGCTATTGATAGAGTTGTTGCTGCTATTTTAGAAAATTATCAAAACCCAGATGGTTCAATCACAATTCCAAAAGTTTTAGTTCCATACATGGGCAAAGAAGTTATTAAATAA", "aa": "MNGSGLAIDRVVAAILENYQNPDGSITIPKVLVPYMGKEVIK", "aa_hexdigest": "daddee63f10a648e665742e1012dd38b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_M4S1T5", "query_cov": 0.8809523809523809, "subject_cov": 0.8043478260869565, "identity": 0.649, "score": 54.7, "evalue": 3.78e-09, "valid": false, "product": "Seryl-tRNA(Ser/Sec) synthetase", "uniref50_id": "UniRef50_M4S1T5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4515.254900000001, "isoelectric_point": 5.97095890045166 }, "id": "DFOKDJOJIG_1059", "locus": "GCA013367855_01058" }, { "type": "cds", "sequence": "contig_1", "start": 604628, "stop": 604744, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATGATGCCAAAAGCATTCTTGAATTGCTTGAAATTCCATACCGTGAAATGATGCTTTCAACTGGCGATATTGGATTTAGCTCAGCAAAAACCATTGATTTGGAACTATGA", "aa": "MVNDAKSILELLEIPYREMMLSTGDIGFSSAKTIDLEL", "aa_hexdigest": "57b07484f1a654554c468177557b5823", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4244.898999999999, "isoelectric_point": 4.196388816833497 }, "id": "DFOKDJOJIG_1060", "locus": "GCA013367855_01059" }, { "type": "cds", "sequence": "contig_1", "start": 604751, "stop": 604906, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGTCTAAGCCAAAGAAATTTGTTGCATATACAAAATGTTTCCGTTCAGAAGCAGGAAGCAGTGGAAAAGATACAAAAGGTTTAATTAGACAACACGAATTTCATAAAGTCGAACTTGTAAAAATAACCAGTGAAAAAGATGCAATGAAAGAATGA", "aa": "MSKPKKFVAYTKCFRSEAGSSGKDTKGLIRQHEFHKVELVKITSEKDAMKE", "aa_hexdigest": "1bf5c3511f53ff80841e97b1b2e2591e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 46, "aa_cov": 0.9019607843137255, "hmm_cov": 0.9130434782608695, "evalue": 4.7683858622369744e-11, "score": 38.68495178222656, "start": 4, "stop": 49 } ], "seq_stats": { "molecular_weight": 5830.716199999999, "isoelectric_point": 9.61141414642334 }, "id": "DFOKDJOJIG_1061", "locus": "GCA013367855_01060" }, { "type": "cds", "sequence": "contig_1", "start": 604964, "stop": 605644, "strand": "-", "frame": 3, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "COG:COG0172", "COG:J", "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_Q98RH5", "UniRef:UniRef90_D1J8K7" ], "nt": "ATGTTAGATTTAAAATATATTATTAGTAACAAAAATGAAGTTATTAAAAAACTATCAACACGTAATTTCAATATTGCTATTATCGAAGAAATTTTTGAAGACGCTCAAAAAAGAAACGAATTAATTCATAATTTAGAAACTTTGCAAGCCAAAAGAAATAAATTAAGTGAACAAATTGGAATTAATAAAAGAAACAAGCAAGATTGCTCAGAATTATTACAACAAGTTGCTGAAGTTAAAGAACAAATCGAAAAAATTGATGAAGAAGCCGACACATTGGTAAAGAAAGTCAATACATTGCTATACCAAATTCCTAACTTGCCATATGATGAAGTTCCTGTGGGAAGAGATGAAGATGACAACGTAGTTTTAAAAGAACATCCAAATTTAGGAAGAGGGTTAGTAAAAGGCGTATTGCCACATTATGAAATAGCGACAAAATTAGATATTGTTGATTTTGTTAGATCAGTGAAGTTAGCAAGCACAAGATTTGTTTTATACAAAAATGATGGTGCTAAATTAGTAAGATCCTTAGCCTCTTTTATGCTTGATACGCACATTGCAAATGGATATAAAGAATTAATGCCCGCCCATTTAGTTAATTCAAATATGCTTTATGGAACGGGACAATTGCCTAAATTTGCAGAAGATTTATTTAAAATGCAAGGCAATGACCTTTGA", "aa": "MLDLKYIISNKNEVIKKLSTRNFNIAIIEEIFEDAQKRNELIHNLETLQAKRNKLSEQIGINKRNKQDCSELLQQVAEVKEQIEKIDEEADTLVKKVNTLLYQIPNLPYDEVPVGRDEDDNVVLKEHPNLGRGLVKGVLPHYEIATKLDIVDFVRSVKLASTRFVLYKNDGAKLVRSLASFMLDTHIANGYKELMPAHLVNSNMLYGTGQLPKFAEDLFKMQGNDL", "aa_hexdigest": "799214d340c25b0e645f110351528f7b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "serS" ], "truncated": "5-prime", "pseudogene": { "start": 604661, "stop": 605644, "observations": { "mutation": [ 604753, 604966 ], "stop": [ 604753, 604966 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K7", "query_cov": 1.0, "subject_cov": 0.5368171021377672, "identity": 1.0, "score": 376.0, "evalue": 8.29e-128, "gene_start": 1, "gene_end": 226, "reference_start": 1, "reference_end": 226, "reference_sequence": "MLDLKYIISNKNEVIKKLSTRNFNIAIIEEIFEDAQKRNELIHNLETLQAKRNKLSEQIGINKRNKQDCSELLQQVAEVKEQIEKIDEEADTLVKKVNTLLYQIPNLPYDEVPVGRDEDDNVVLKEHPNLGRGLVKGVLPHYEIATKLDIVDFVRSVKLASTRFVLYKNDGAKLVRSLASFMLDTHIANGYKELMPAHLVNSNMLYGTGQLPKFAEDLFKMQGNDLWLIPTAEVPVTNYYYDEILDLSKPKKFVAYTKCFRSEAGSSGKDTKGLIRQHEFHKVELVKITSEKDAMKEWESMVNDAKSILELLEIPYREMMLSTGDIGFSSAKTIDLELWIPSEQRYRETSSISICRDFQGRRAKIRYRDETGHTKYAYTMNGSGLAIDRVVAAILENYQNPDGSITIPKVLVPYMGKEVIK" }, "paralog": false, "identity": 0.9938837920489296, "score": 575.0, "evalue": 8.24e-209, "description": "Internal stop codon at 604753, 604966. Nonsense mutation around 604753, 604966.", "psc": { "uniref90_id": "UniRef90_D1J8K7", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_Q98RH5", "cog_id": "COG0172", "cog_category": "J", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K7", "EC:6.1.1.11", "UniRef:UniRef50_Q98RH5", "COG:COG0172", "COG:J", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RH5" ], "product": "Serine--tRNA ligase" } }, "id": "DFOKDJOJIG_1062", "locus": "GCA013367855_01061" }, { "type": "cds", "sequence": "contig_1", "start": 605919, "stop": 606272, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCTGTTAAAGTTAATAAATACAACAGTATTTCAGTAACTTTAAAAAACAATTTATTTTCTGAAAAAAACATTACTTTAAATAGCCACATTTATGAAATATCAGTCTCTAAAAATAGTTTTTCAAAATATCCAAATAGTTATATTGCAACAGACGTTATTGAAATAAACGAAGATGAAGAATATAAACTAAACTATGTTATTAAATTTTTAAAAAGCAGTCATTTCCCTGGTATAGGCGAAGAAAAAGCAAAATTAATTGTAAAAGATTTAGGGATTGACGCTTTAAAAAAAATGGTTGAGGATAATTCAATTGATTATTCGAAATATTCAATATCAGAATTGACATGA", "aa": "MNPVKVNKYNSISVTLKNNLFSEKNITLNSHIYEISVSKNSFSKYPNSYIATDVIEINEDEEYKLNYVIKFLKSSHFPGIGEEKAKLIVKDLGIDALKKMVEDNSIDYSKYSISELT", "aa_hexdigest": "0ca4b27ec176948ce58ef2a1b455eaa0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13391.070299999985, "isoelectric_point": 6.1115217208862305 }, "id": "DFOKDJOJIG_1063", "locus": "GCA013367855_01062" }, { "type": "cds", "sequence": "contig_1", "start": 606324, "stop": 606635, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTTTTAACAAATAATTTAACACCTTCTTTGTATGAAATAGTTGCTAAGAAATTTGAACATTTTAAAGATTTTATAGATGCTTACAAAGATAACTTTTACTTATTTTATTTAGACAATGAACGCATCTCGCTTGAAGATATGGACAAATTACATAATATCTTTAACAAATTTGAACATCCTTTTAAGCCTGCAACTCACTTGTACAAATCATTTTTAAAATATCTATATAATTCTGGAAACACTAGAATTTTAAAAGATTATTTATACGACCAACTAATAAACTTTGCAAGCCAAGACACTGAATGA", "aa": "MFFLTNNLTPSLYEIVAKKFEHFKDFIDAYKDNFYLFYLDNERISLEDMDKLHNIFNKFEHPFKPATHLYKSFLKYLYNSGNTRILKDYLYDQLINFASQDTE", "aa_hexdigest": "bbd10f8aa144e225834635c5cbecca17", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12574.095599999982, "isoelectric_point": 5.754743385314941 }, "id": "DFOKDJOJIG_1064", "locus": "GCA013367855_01063" }, { "type": "cds", "sequence": "contig_1", "start": 606675, "stop": 608057, "strand": "+", "frame": 3, "gene": "recD", "product": "Exodeoxyribonuclease V alpha chain", "db_xrefs": [ "COG:COG0507", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J8K8", "UniRef:UniRef90_D1J8K8" ], "nt": "TTGGTTCAAAATAAATATTTAATCGAGATTGAATACGAAGATGGTACATACCTTAGTGCTAAAGATGTATTTGATATGGAAAAATATATTGTCAAAAGATTGAAACATATTGAATCTTCTTCAAACCCAAAATTCATCAATTTTATGCCAAGCGAAATGTTCCACCCTTTGCAACTTCAAGCAATAGAAACAGCACTAACTAGCAAATTGACATTGATTACAGGAAACCCTGGCACTGGAAAAACATTGATAACTAATGAAATAATTAAACAACTGCTTTCAGTCTATGATGAAGATGATTTGGCAATAGTTACACCAACCGGTAGAGCAACTATCAACATAAACAATATTCAAGAAAAGACGAATGCCGTTACCATACATAGTTTTTTAGAATGGGATGTTGATAATGACAAATTCATGATTAATGAAAAAAATGCAAAACCGATTGAATGTTTAATAATTGATGAATTTTCAATGGTAAGTGTTGATTTATTTTATTCACTACTTAAAGGAATAAAAAAGAATTCATTAAAACGAATCATATTAGTTGGCGACAAAGATCAATTGCCTGCCATTGGCGCTGGTTATTTAATTAGGGATTTTATAGAAAGCAATATTTTTCAAACAATTGTTTTAAGTAAAATTTTTAGACAAGCAGAAAATTTTGACATTATTCAAGATGCTCTAGATATTAATAAAGGCAAACTACCCGAATTTAAAGGAAATAATTCCCAATTTATTGAAACGAAACAGAGTGATCTAAAGTCAATGTTACTCTTTAAATTAAATGACTTATTGCAACAAGGCTATAGCAAGCGTGATATTGCAATTTTAAGTCCAATATATAATTACGAAACGGGAATTGATCAAATAAATGAAACATTAAATAATTTCTTTAGAAAATTAGATGGCAATGAACAAATAAAGTATCGAGACCATGTTTTGGCCATTGATGATAAGGTAATAAATTTAGTTAATGATCCAAAAACAAAAGTGTTTAATGGAGAAATCGGTTATATATCTCACTTTGGCTTTGAAAACAAAAAAAATTCATTTGAAAAGGTTTTAGCAAGTATCTCTGTAGATTTTGAAAACGATTCAAAAACTGTTTCTTATTCTAGAAAAGATTTTTTTGCTAATACATATCCTGCATATTGCACAAGCGTTCACAAATATCAAGGTAGTGAATGTAAATGCGTTATTGTAGTATTATTTTCAGAGGCTAAAAAATTATTATCTAAAAAACTTATTTATACCGCTATTACTAGAGCAAAAAAATATTGCGTTGTTATTGGAGAAAAAGAAGCTTTGATAACCGGTATAAATAATGATGATGATTCAAATAGAATAACCAATATTAAATATTTATGGAGGAATGAATAA", "aa": "MVQNKYLIEIEYEDGTYLSAKDVFDMEKYIVKRLKHIESSSNPKFINFMPSEMFHPLQLQAIETALTSKLTLITGNPGTGKTLITNEIIKQLLSVYDEDDLAIVTPTGRATININNIQEKTNAVTIHSFLEWDVDNDKFMINEKNAKPIECLIIDEFSMVSVDLFYSLLKGIKKNSLKRIILVGDKDQLPAIGAGYLIRDFIESNIFQTIVLSKIFRQAENFDIIQDALDINKGKLPEFKGNNSQFIETKQSDLKSMLLFKLNDLLQQGYSKRDIAILSPIYNYETGIDQINETLNNFFRKLDGNEQIKYRDHVLAIDDKVINLVNDPKTKVFNGEIGYISHFGFENKKNSFEKVLASISVDFENDSKTVSYSRKDFFANTYPAYCTSVHKYQGSECKCVIVVLFSEAKKLLSKKLIYTAITRAKKYCVVIGEKEALITGINNDDDSNRITNIKYLWRNE", "aa_hexdigest": "f2e6999fddcc00092c549b194edc5702", "start_type": "TTG", "rbs_motif": null, "genes": [ "recD" ], "truncated": "5-prime", "pseudogene": { "start": 606375, "stop": 608057, "observations": { "mutation": [ 606633 ], "stop": [ 606633 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K8", "query_cov": 1.0, "subject_cov": 0.6157965194109772, "identity": 0.996, "score": 903.0, "evalue": 0.0, "gene_start": 1, "gene_end": 460, "reference_start": 288, "reference_end": 747, "reference_sequence": "MLQNSNIQGKFNKIMWTSTDGQVVIVSFLVSSNDGLNPVKVNKYNSISVTLKNNLFSEKNVTLNSHIYEISVSKNSFSKYPNSYIATDVIEINEDEEYKLNYVIKFLKSSHFPGIGEEKAKLIVKDLGIDALRKMVEDNSIDYSKYSISELTWNYAVEFIKNNPTLIEDQMFFLTNNLTPSLYEIVAKKFEHFKDFIDAYKDNFYLFYLDNERISLEDMDKLHNIFNKFEHPFKPATHLYKSFLKYLYNSGNTRILKDYLYDQLINFASQDTEWPRDVETFNNSVELLVQNKYLIEIEYEDGTYLSAKDVFDMEKYIVKRLKHIESSSNPKFINFMPSEMFHPLQLQAIETALTSKLTLITGNPGTGKTLITNEIIKQLLSVYDEDDLAIVTPTGRATININNIQEKTNAVTIHSFLEWDVDNDKFMINEKNAKPIECLIIDEFSMVSVDLFYSLLKGIKKNSLKRIILVGDKDQLPAIGAGYLIRDFIESNIFQTIVLSKIFRQAENFDIIQDALDINKGKLPEFKGNNSQFIETKQSDLKSMLLFKLNDLLQQGYSKRDIAILSPIYNYETGIDQINETLNNFFRKLDGNEQIKYRDHILAIDDKVINLVNDPKTKVFNGEIGYISHFGFENKKNSFEKVLASISVDFENDSKTVSYSRKDFFANTYPAYCTSVHKYQGSECKCVIVVLFSEAKKLLSKKLIYTAITRAKKYCVVIGEKEALITGINNDDDSNRITNIKYLWRNE" }, "paralog": false, "identity": 0.9964285714285714, "score": 1115.0, "evalue": 0.0, "description": "Internal stop codon at 606633. Nonsense mutation around 606633.", "psc": { "uniref90_id": "UniRef90_D1J8K8", "gene": "RecD", "product": "Exodeoxyribonuclease V alpha chain", "uniref50_id": "UniRef50_D1J8K8", "cog_id": "COG0507", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K8", "UniRef:UniRef50_D1J8K8", "COG:COG0507", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8K8" ], "product": "Exodeoxyribonuclease V alpha chain" } }, "id": "DFOKDJOJIG_1065", "locus": "GCA013367855_01064" }, { "type": "cds", "sequence": "contig_1", "start": 608057, "stop": 608617, "strand": "+", "frame": 2, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "RefSeq:WP_012855695.1", "SO:0001217", "UniParc:UPI0001BC2435", "UniRef:UniRef100_D1J8K9", "UniRef:UniRef50_Q98PE2", "UniRef:UniRef90_D1J8K9" ], "nt": "ATGAAATTAATTGTTGGACTTGGAAATCCTGGTGTTGAATATGAAAAAACACGCCACAACACAGGGTTTATGGTAATTGACAAACTAGCAGAAAAATTGCATACTCCCTTAACTGAAAAAAAGTTTAATGGAATTTATTACATTGACAAAGAAGTTATTTTAGCCAAACCGTTAACCTTTATGAATAATAGCGGTGATTTTGTTAGAGCAATAAAGGATTATTACAATATTGTAGATGATGACATTATCATTGTATATGATGATTTAGATTTGCCAGTTGGAAAAGCTGTTATTAGACAAAAAGGTTCGTCTGGCGGACATAACGGAATGAAAGATATAATTGCTAAATTAAAAACTGATGAAATAAAAAGATTAAAAATAGGAATTGGTCGTGGTCAGAATGTAATTAATTATGTTCTTGGCAAATTTAGCTATCAAGATTTTTGCACAATTGAAAAAATAATTGACATTGCTTCCGATGCATTAATTTCTTTTATTAGTAATGATATTAGATTTGTTATGAATAAATTTAGTGGTGAACTATATCGTGAAAACAAATAA", "aa": "MKLIVGLGNPGVEYEKTRHNTGFMVIDKLAEKLHTPLTEKKFNGIYYIDKEVILAKPLTFMNNSGDFVRAIKDYYNIVDDDIIIVYDDLDLPVGKAVIRQKGSSGGHNGMKDIIAKLKTDEIKRLKIGIGRGQNVINYVLGKFSYQDFCTIEKIIDIASDALISFISNDIRFVMNKFSGELYRENK", "aa_hexdigest": "ffa6914d0b626ec4bf14b6ed375ee7a8", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001BC2435", "ncbi_nrp_id": "WP_012855695.1", "uniref100_id": "UniRef100_D1J8K9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2435", "RefSeq:WP_012855695.1", "UniRef:UniRef100_D1J8K9" ] }, "ips": { "uniref100_id": "UniRef100_D1J8K9", "uniref90_id": "UniRef90_D1J8K9" }, "psc": { "uniref90_id": "UniRef90_D1J8K9", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_Q98PE2", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PE2" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "DFOKDJOJIG_1066", "locus": "GCA013367855_01065" }, { "type": "cds", "sequence": "contig_1", "start": 608604, "stop": 609038, "strand": "+", "frame": 3, "gene": "ttcA", "product": "tRNA(Ile)-lysidine synthase", "db_xrefs": [ "COG:COG0037", "COG:J", "EC:6.3.4.19", "KEGG:K04075", "SO:0001217", "UniRef:UniRef50_D1J8L0", "UniRef:UniRef90_D1J8L0" ], "nt": "GTGAAAACAAATAATTTAGTTGAATTACAACATGATTTATTAAAAGAATTTAATAAATACAATATTGATACCAATAAAAAGTTTTTAATCGGAGTAAGTGGTGGCCCTGATTCAATGTGGCTACTTAATTTGCTTAAATCATTGAATATTGTTGTTGCGCATGTAAACTACAATAAAAGATATGATAGCAATTATGATGAATCATTGGTTTCACGATTTTGTGCTGATAACAATATCCCTTTAGAAATACTCTCATTACATAACAAATATTCTAGTGGTAACTTCCAAGCTATTGCTAGAAAAGAAAGATATGATTTTTACTTTGAAATTTCTAAAAAATATCAATGCGACTATATCCTCCTTGCTCACCAAAAAGATGATTTTTTAGAATCATCTATTATGCAAGAAAATTCAAACAGAAAACCCTTATTTTGA", "aa": "MKTNNLVELQHDLLKEFNKYNIDTNKKFLIGVSGGPDSMWLLNLLKSLNIVVAHVNYNKRYDSNYDESLVSRFCADNNIPLEILSLHNKYSSGNFQAIARKERYDFYFEISKKYQCDYILLAHQKDDFLESSIMQENSNRKPLF", "aa_hexdigest": "a6efd2ec93477a21ea813ef0f97f2458", "start_type": "GTG", "rbs_motif": null, "genes": [ "ttcA" ], "truncated": "3-prime", "pseudogene": { "start": 608604, "stop": 609341, "observations": { "mutation": [ 609036, 609099, 609315 ], "stop": [ 609036, 609099, 609315 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L0", "query_cov": 1.0, "subject_cov": 0.4630225080385852, "identity": 0.972, "score": 283.0, "evalue": 2.26e-94, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MKTNNLVELQRDLLNEFNKCNIDTNKKFLIGVSGGPDSMWLLNLLKSLNIVVAHVNYNKRYDSNYDESLVSQFCADNNIPLEILSLHNKYSSGNFQAIARKERYDFYFEISKKYQCDYILLAHQKDDFLESSIMQENSNRKPLFWGIKQLSVLNNNIIFRPMLNLWTKQEIIDFCNEYSILFATDYTNAETHYTRNKIRSNLKKLDPEQKQNKYQFFVNKNIENEHIEKQVLESFEDWKNNQFNVDKLDKNFVYIDQLLFYTLNHFYNNLKLSLNKIHGIKDFLFSQNRTKNFKLNDEQYIHKIKNLLIFK" }, "paralog": false, "identity": 0.9673469387755103, "score": 477.0, "evalue": 5.75e-173, "description": "Internal stop codon at 609036, 609099, 609315. Nonsense mutation around 609036, 609099, 609315.", "psc": { "uniref90_id": "UniRef90_D1J8L0", "gene": "TtcA", "product": "tRNA(Ile)-lysidine synthase", "ec_ids": [ "6.3.4.19" ], "uniref50_id": "UniRef50_D1J8L0", "cog_id": "COG0037", "cog_category": "J", "kegg_orthology_id": "K04075", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L0", "EC:6.3.4.19", "UniRef:UniRef50_D1J8L0", "COG:COG0037", "COG:J", "KEGG:K04075" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8L0" ], "product": "tRNA(Ile)-lysidine synthase" } }, "id": "DFOKDJOJIG_1067", "locus": "GCA013367855_01066" }, { "type": "cds", "sequence": "contig_1", "start": 609586, "stop": 609795, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAATCCAAAAGAAAAAAAACCTAGGAAAATTGGTTTGTTTGGAATAATTGGAATTTTATTTGCAATTATTGCAGTTATAACATTGGGATATATTATCAGTGATGTTGTTAAAGAAAGAATGTCTAACTTTCAAACCTTAAATTTTCTAGAAAATGTTATTAGAGGAACTGCTAACAAAAATGATGGAAACTATATTTCTTCATGA", "aa": "MNNPKEKKPRKIGLFGIIGILFAIIAVITLGYIISDVVKERMSNFQTLNFLENVIRGTANKNDGNYISS", "aa_hexdigest": "7b75372bc2f7ddd2b075f58d089ff3ee", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7699.945, "isoelectric_point": 9.77445468902588 }, "id": "DFOKDJOJIG_1068", "locus": "GCA013367855_01067" }, { "type": "cds", "sequence": "contig_1", "start": 609994, "stop": 611295, "strand": "+", "frame": 1, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A485JC26" ], "nt": "TTGAATTCTTGTGCAACATTTAATGGTCAAGTTTTAGGAAATATTGTTGCAAGTTCAATGCCAAAGCCAAGCATTGCAAGCAAATATATAATTCCTGCTTTCTCTGCTTTTGTTGGCTTAGCATTTATTATTATCATTATGGCAATGTATTTTGGTAAGGGAAGAGGAATGCTAACTGGCGGTATTGATACAAACAAATCATTAGCGCAAAGAATATATTCAAATAAAAAATTCTCTGACATTGCCGGAAATGAAGAAGTAAAAGAAGAAGTAAAAGAATTAGTTGATTATCTTAAAAATCCTAAAAAATATGAAGCAGCTGGCGCTAGAATTCCAAAAGGAATATTACTAGGAGGCCCTCCAGGAACTGGTAAAACTTTAATTGCCAAAGCAACAGCGGGAGAAGCAAACGTTCCATTCTTCTTCATCTCGGCATCAAATTTTGTTGAAATGTTTGTTGGATTGGGCGCAAAAAGAGTTCGTGACATGTTTGCTGAAGCTCGCTCTCATGCTCCTGCAATAATTTTCATTGATGAATTAGATGCTGTTGGTAGATCTCGTGGAGCTGGAATTGGTGGAGGTAATGACGAAAGAGAACAAACTCTTAACCAATTACTAGTAGAGATGGATGGTATCAAAGAAAATAGCGGTATTTTAATAATGGCTGCTACAAATAGAACCGATGTTCTAGACCCCGCTTTATTAAGACCAGGACGTTTTGATAGAACTATTACCGTTGGCCTTCCTGATATAAAAGAAAGAGAAGCAATCTTAAAATTACATGCTAATGGAAAAAGAATTGCAAACAATATTAATTTTGCTATGGTAGCAAGAAGAACACCTGGTTTCTCTGGAGCACAGCTTGAAAACGTAATTAACGAAGCCTCTTTATTAAGTGTTAGAGAAAATACCGGAGTTATAACTATTGAACAAATAGATGAAGCTATTGACAGAGTTATTGCCGGCCCTGCTAAAAAATCAAGAACCATTTCTGAAAAAGAAAACATTGCAGTTGCCTACCATGAAGCGGGACACGCAGTTGTTGGAATAAAAATTAAAGGTGGAAATAAAGTTCAAAAAATAACCATCATTCCAAGAGGAAAAGCTGGCGGATATAATTTAATGATGCCTGATGAAGAAAAATACAATCGCTCGAAATCAGAATTACTAGCTATAATAACTTCGTTTATGGGAGGACGTGTAGCTGAAGCAATTATTTATGGAGCAGATAATGTTTCAACTGGAGCAAGTGATGATATTGCCAAAGCAACAAATATTGCTAGAAAAATGGTTACTGAATGA", "aa": "MNSCATFNGQVLGNIVASSMPKPSIASKYIIPAFSAFVGLAFIIIIMAMYFGKGRGMLTGGIDTNKSLAQRIYSNKKFSDIAGNEEVKEEVKELVDYLKNPKKYEAAGARIPKGILLGGPPGTGKTLIAKATAGEANVPFFFISASNFVEMFVGLGAKRVRDMFAEARSHAPAIIFIDELDAVGRSRGAGIGGGNDEREQTLNQLLVEMDGIKENSGILIMAATNRTDVLDPALLRPGRFDRTITVGLPDIKEREAILKLHANGKRIANNINFAMVARRTPGFSGAQLENVINEASLLSVRENTGVITIEQIDEAIDRVIAGPAKKSRTISEKENIAVAYHEAGHAVVGIKIKGGNKVQKITIIPRGKAGGYNLMMPDEEKYNRSKSELLAIITSFMGGRVAEAIIYGADNVSTGASDDIAKATNIARKMVTE", "aa_hexdigest": "cbe20e98ba28c4bcbee3448936312e36", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A485JC26", "query_cov": 0.9468822170900693, "subject_cov": 0.8102766798418972, "identity": 0.512, "score": 403.0, "evalue": 5.68e-134, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "ec_ids": [ "3.4.24.-" ], "uniref50_id": "UniRef50_A0A485JC26", "go_ids": [ "GO:0004176", "GO:0004222", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_A0A485JC26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A485JC26" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "DFOKDJOJIG_1069", "locus": "GCA013367855_01068" }, { "type": "cds", "sequence": "contig_1", "start": 611365, "stop": 611805, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01434.24" ], "nt": "ATGAAAAATTCTTCATTTTCAAGTAAAGTAGGCCAAGAAATTGATGAACAAATAAGAAAAATTATTTTAACCGCCGAAAAGAAAGCACATGAAATAATTACAGAAAATCGTCAATTGTTAGAATTAATTAAAGACGCATTAATTATTAAAGAAACTATTGTTGCTGAAGAAATTGAATATATAGAAAAACATATGGAATTACCTCCTGAAATAACAAATACAAAAAAAGAACTTAAAAATGAATATTCAGAAGAAGATTTCAATGAATTATTTAATGAAGTTGCTGGACAAAAACAAATTCAAGAAGATAAATACAAAGCTACTTTAAACTCTGAATTAAAGAAACATAATATTGAAGATAATGATTCAAGCAACCATTCAGAAGATTCTAATAATACAAAAAATTCAGATGAAGACAGCAACAAAGAAGACAATCAATAA", "aa": "MKNSSFSSKVGQEIDEQIRKIILTAEKKAHEIITENRQLLELIKDALIIKETIVAEEIEYIEKHMELPPEITNTKKELKNEYSEEDFNELFNEVAGQKQIQEDKYKATLNSELKKHNIEDNDSSNHSEDSNNTKNSDEDSNKEDNQ", "aa_hexdigest": "b9ced80b2258dd92d3c4d20de8833d60", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01434.24", "name": "Peptidase_M41", "length": 59, "aa_cov": 0.4041095890410959, "hmm_cov": 1.0, "evalue": 8.744957110787963e-11, "score": 37.650604248046875, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 16980.43399999999, "isoelectric_point": 4.682134819030761 }, "id": "DFOKDJOJIG_1070", "locus": "GCA013367855_01069" }, { "type": "cds", "sequence": "contig_1", "start": 611831, "stop": 612184, "strand": "-", "frame": 3, "gene": "ygaC", "product": "DUF402 domain-containing protein", "db_xrefs": [ "COG:COG3557", "COG:S", "KEGG:K07586", "SO:0001217", "UniRef:UniRef50_A0AA43TZF8", "UniRef:UniRef90_D1J8L2" ], "nt": "TTGTTTTTCTTTGCTAGAAACAAATTTTATAATGCTTCTATTACATTAAATAAATCTAAAGAAAACTATATTTATATTAATTTAGCAAGCCCTTTTTATATTGAAAATGAAGTAATTAAATATATTGATTTTGATATCGATATTAAAAGTTATGATGATAAAGATTTTAGCGTTATAGATTGGCAAGATTTTAAAAATTCAATTAGTCAATACAATTATTCATTGAAATTAATTTATAAATTGTATGAAGAATTAGATTTTTTATATGAACAAAAGCAAAAAGGAACAGGAGTTTTTTCTAAGAAACTTGTTAACGGCCTTGAAAAGATGTTGCGAGAAAGCGGAGATATATAA", "aa": "MFFFARNKFYNASITLNKSKENYIYINLASPFYIENEVIKYIDFDIDIKSYDDKDFSVIDWQDFKNSISQYNYSLKLIYKLYEELDFLYEQKQKGTGVFSKKLVNGLEKMLRESGDI", "aa_hexdigest": "3b42402dbf2f9f440d25f246ec1fd3a3", "start_type": "TTG", "rbs_motif": null, "genes": [ "ygaC" ], "truncated": "3-prime", "pseudogene": { "start": 611831, "stop": 612445, "observations": { "mutation": [ 612205 ], "stop": [ 612205 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L2", "query_cov": 1.0, "subject_cov": 0.5735294117647058, "identity": 0.991, "score": 228.0, "evalue": 2.52e-74, "gene_start": 1, "gene_end": 117, "reference_start": 88, "reference_end": 204, "reference_sequence": "MEEESIKSFDDLLKTIDTPNSKNIKKHKKFFKMGQLTTIQVFKYDGTLYRQYEGAKIVANLTDFVVVLLVKTKVSEKSINWVVSEPILFFFARNKFYNASITLNKSKENYIYINLASPFYIENEVIKYIDFDIDIKSYDDKDFSVIDWQDFKNSISQYNYSLKLIYKLYEELDFLYEQKQKGTGVFSKKLVNGLEKMLRESGDI" }, "paralog": false, "identity": 0.9901960784313726, "score": 401.0, "evalue": 4.15e-145, "description": "Internal stop codon at 612205. Nonsense mutation around 612205.", "psc": { "uniref90_id": "UniRef90_D1J8L2", "gene": "YgaC", "product": "DUF402 domain-containing protein", "uniref50_id": "UniRef50_A0AA43TZF8", "cog_id": "COG3557", "cog_category": "S", "kegg_orthology_id": "K07586", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L2", "UniRef:UniRef50_A0AA43TZF8", "COG:COG3557", "COG:S", "KEGG:K07586" ] }, "pscc": { "uniref50_id": "UniRef50_A0AA43TZF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA43TZF8" ], "product": "DUF402 domain-containing protein" } }, "id": "DFOKDJOJIG_1071", "locus": "GCA013367855_01070" }, { "type": "cds", "sequence": "contig_1", "start": 612203, "stop": 612445, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGAAAGCATAAAGAGTTTTGATGATTTATTAAAAACAATTGATACACCAAACTCAAAAAATATAAAAAAACATAAAAAGTTTTTTAAGATGGGACAACTAACAACCATTCAAGTTTTTAAATATGATGGAACTTTATACAGACAATATGAAGGGGCAAAAATAGTTGCAAATTTAACTGACTTTGTAGTTGTTTTGTTAATGAAAACAAAAGTTTCTGAAAAATCTATCAATTGA", "aa": "MEEESIKSFDDLLKTIDTPNSKNIKKHKKFFKMGQLTTIQVFKYDGTLYRQYEGAKIVANLTDFVVVLLMKTKVSEKSIN", "aa_hexdigest": "94f70607d96c17d2aa44eef453fc226f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9277.7353, "isoelectric_point": 9.363597679138184 }, "id": "DFOKDJOJIG_1072", "locus": "GCA013367855_01071" }, { "type": "cds", "sequence": "contig_1", "start": 612699, "stop": 612965, "strand": "+", "frame": 3, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_Q9F411", "UniRef:UniRef90_Q9F411" ], "nt": "ATGGCAAGCCCAATGACATTTAAGGATTTTCCTATTGATAATAAAAAAAATACTATATTAGAGATTGGTATGGGGAGAGGAACCATGCTTACAAAATTAGCCCTAATGCACCCTGACATAGAATATATCGGACTTGAAAAATACAGCACGCCCGCCTATTCTGCTTTAAAAAAAGCAATTGACCTCAACCTTGAAAATTTTCACATAATTATAGGAGATGCAATAAATTTATCAACTTATTTTAAAAACAAAATTAAAACTATTTGA", "aa": "MASPMTFKDFPIDNKKNTILEIGMGRGTMLTKLALMHPDIEYIGLEKYSTPAYSALKKAIDLNLENFHIIIGDAINLSTYFKNKIKTI", "aa_hexdigest": "b82d40745df411e9eea4445fba71bb60", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 612660, "stop": 613268, "observations": { "mutation": [ 612963, 612984 ], "stop": [ 612963, 612984 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9F411", "query_cov": 1.0, "subject_cov": 0.43564356435643564, "identity": 1.0, "score": 179.0, "evalue": 1.47e-55, "gene_start": 1, "gene_end": 88, "reference_start": 14, "reference_end": 101, "reference_sequence": "MRLRFNKNAETSLMASPMTFKDFPIDNKKNTILEIGMGRGTMLTKLALMHPDIEYIGLEKYSTPAYSALKKAIDLNLENFHIIIGDAINLSTYFKNKIKTIWLTFSDPWPKKRHYKRRLVYRDFLKIYQNVLDKDGVVYFKTDNDMLYQFAIDELKEINAKIIYQTSDLHHCNFKIENVFTDYEEKFNKLNKNINFIAFTFN" }, "paralog": false, "identity": 0.9801980198019802, "score": 402.0, "evalue": 6.28e-146, "description": "Internal stop codon at 612963, 612984. Nonsense mutation around 612963, 612984.", "psc": { "uniref90_id": "UniRef90_Q9F411", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_Q9F411", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9F411", "EC:2.1.1.33", "UniRef:UniRef50_Q9F411", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_Q9F411", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9F411" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "DFOKDJOJIG_1073", "locus": "GCA013367855_01072" }, { "type": "cds", "sequence": "contig_1", "start": 613068, "stop": 613268, "strand": "+", "frame": 3, "gene": null, "product": "tRNA (Guanine-N(7)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PL40" ], "nt": "GTGGTTTATTTTAAAACCGATAATGATATGCTTTATCAATTTGCAATAGATGAATTAAAAGAAATCAATGCAAAAATCATTTATCAAACTTCTGATTTACACCATTGCAATTTTGAAATAGAAAATGTTTTTACTGATTATGAAGAAAAATTTAACAAATTAAACAAAAATATAAACTTTATTGCGTTTACTTTTAATTAA", "aa": "MVYFKTDNDMLYQFAIDELKEINAKIIYQTSDLHHCNFEIENVFTDYEEKFNKLNKNINFIAFTFN", "aa_hexdigest": "36e27a7026d4feab4277b0b28476646a", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0PL40", "query_cov": 0.9848484848484849, "subject_cov": 0.8441558441558441, "identity": 0.615, "score": 93.6, "evalue": 8.69e-24, "valid": false, "product": "tRNA (Guanine-N(7)-)-methyltransferase", "uniref50_id": "UniRef50_A0A3B0PL40" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PL40", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PL40" ], "product": "tRNA (Guanine-N(7)-)-methyltransferase" }, "genes": [], "id": "DFOKDJOJIG_1074", "locus": "GCA013367855_01073" }, { "type": "cds", "sequence": "contig_1", "start": 613343, "stop": 615016, "strand": "+", "frame": 2, "gene": "gyrA", "product": "DNA gyrase subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265", "KEGG:K02469", "SO:0001217", "UniRef:UniRef50_B3PN30", "UniRef:UniRef90_D1J8L4" ], "nt": "ATGCTTTTTGGCGAAGATGATAACAATAAAAAAGAAAACAAAAATTTTGAAGAAGATAACAGCCACGATTTAGATGAATATACAAATTTTGATGATAGAATCAAAAAAATCTTTAGTGACGATGATGAAACAAAAGCAAAAAAAATAGACGAAGAGGACGAAGAAGAAATCCCTCAAGACAAAGAAGGCTATAAGGTAGAACCCCAACTTTTAGATAAAGAAATTAATGGTTTAAGACCCGCTAATTTATCAAAAGTAATGAAAACATCATTCATTGAATATGCAATGAGTGTCATAGTTTCACGTGCTCTACCTGATGCTCGTGATGGTCTAAAGCCAGTACACCGTAGAATTTTATATGGCATGAGCGAACTTGGAATGTTTTATACAGCGCCACATAAAAAATCAGCAAGAATCGTCGGAGATGTTTTAGGTAAATATCACCCACATGGAGATTCATCAGTATATGAAGCTATGGTAAGAATGGCTCAAGATTTTTCACTTCGCTATCCTTTAATTGATGGACATGGTAACTTTGGATCTGTTGACGGAGACGAAGCTGCTGCAATGCGTTATACAGAAGCTAGAATGAGTAAGATTGCTGGTGCAATGGTTGATGGTATTAAGAAAAATACTGTTGACTTTATGGACAACTATGATGCAACTGAAAAAGAACCAGTTGTTTTACCATCACGTTTCCCTAATTTATTAGTTTCTGGTTCAAGTGGTATTGCTGTTGGTATGGCAACAAATATTCCTCCTCATAACCTGGGCGAAACTATTGATGCAGTTTGTGCACTTGCAAAAAATCCAGAAATAAATGTTTTTGAATTAATGGAATATATTCAAGCTCCTGATTTTCCAACAGGTGGAATAATATTTAATAAAAAAGGATTAATTGAAGCTTATAGCACAGGTAGAGGATCAATCACCATTCGTTCAAAAGCACACATACAAGAACTTTCAAACGGAAAAAGTAAAATAATTGTTACTGAAATTCCATACGAAGTTAAAAAAACAGAAATCATGGAAAAAATTTCTGAACTTATTAAAGATAAAAAAATTGATGGAATTGCTGATTTTAGAGATGAATCGAACAGAGATGGCATTAGAATTGTAATTGATATCAAAAAATCTTTTGTTCCTGAAATTGTTTTAAATAACTTGTTCAAATTAACAAAATTACAAAGCAATTATTCATTCAATATGATTGCGCTGGTAAATAATGAACCAAAATTGTTGAACCTTAAGGCTTGCTTAGAAGTTTATTTAAAACACCAAATTGAAGTTGTAAGTAGAAGATTAAAATTTGATCTTGAAAAAGATAGAGCAAGAGCTCATATTTTAGAAGGTTTAAAAATCTGCATTGAAAATATTGATGCCGTAATTAAAATAATTAAAGAATCGAAAACTGACCAAGAAGCACAAAGCAAACTTTCTCAAGCATTTAATCTTAGTGAAATACAAACAAAAGCAATTGTTGATATGAAGCTAGGAAGATTAACTGGATTAGCAATTGAAAAAATGAACGAAGAACTAAATGAAGTACATGAAAGAATTGCTAACTACATTAAGATATTAGGCGACCATAACTTATTAATCAATTTGATAATTGAAGAATTACAAGAAATTAAAAATGCTTATGGCGATGCTCGTAGAAGCGATATAAATTGA", "aa": "MLFGEDDNNKKENKNFEEDNSHDLDEYTNFDDRIKKIFSDDDETKAKKIDEEDEEEIPQDKEGYKVEPQLLDKEINGLRPANLSKVMKTSFIEYAMSVIVSRALPDARDGLKPVHRRILYGMSELGMFYTAPHKKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSLRYPLIDGHGNFGSVDGDEAAAMRYTEARMSKIAGAMVDGIKKNTVDFMDNYDATEKEPVVLPSRFPNLLVSGSSGIAVGMATNIPPHNLGETIDAVCALAKNPEINVFELMEYIQAPDFPTGGIIFNKKGLIEAYSTGRGSITIRSKAHIQELSNGKSKIIVTEIPYEVKKTEIMEKISELIKDKKIDGIADFRDESNRDGIRIVIDIKKSFVPEIVLNNLFKLTKLQSNYSFNMIALVNNEPKLLNLKACLEVYLKHQIEVVSRRLKFDLEKDRARAHILEGLKICIENIDAVIKIIKESKTDQEAQSKLSQAFNLSEIQTKAIVDMKLGRLTGLAIEKMNEELNEVHERIANYIKILGDHNLLINLIIEELQEIKNAYGDARRSDIN", "aa_hexdigest": "c3d5476bf92d76d63144ddd1ac45c7e4", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0015AA695A", "query_cov": 0.8725314183123878, "subject_cov": 0.8983364140480592, "identity": 0.593, "score": 575.0, "evalue": 7.62e-199, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A2N1QA61" }, "genes": [ "gyrA" ], "truncated": "3-prime", "pseudogene": { "start": 613343, "stop": 615319, "observations": { "mutation": [ 615014 ], "stop": [ 615014 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L4", "query_cov": 1.0053859964093357, "subject_cov": 0.612691466083151, "identity": 0.9840000000000001, "score": 1051.0, "evalue": 0.0, "gene_start": 1, "gene_end": 557, "reference_start": 1, "reference_end": 560, "reference_sequence": "MLFGEDDNNKKENKNFEEDNSHDLDEYTNFDDRIKKIFSDDDETKAKKIDEEDEEDEEEIPQDKEGYKVEPQLLDKEINGLKPANLSKVMKTSFIEYAMSVIVSRALPDARDGLKPVHRRILYGMSELGMFYTAPHKKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSLRYPLIDGHGNFGSVDGDEAAAMRYTEARMSKIAGAMVDGIKKNTVDFMDNYDATEKEPVVLPSRFPNLLVSGSSGIAVGMATNIPPHNLGETIDAVCALAKNPEINVFELMEYIQAPDFPTGGIIFNKKGLIEAYSTGRGSITIRSKAHIQELSNGKSKIIVTEIPYEVKKTEIMEKISELIKDKKIDGIADFRDESNRDGIRIVIDIKKSFVPEIVLNNLFKLTKLQSNYSFNMIALVNNEPKLLNLKACLEVYLKHQIEVVSRRLKFDLEKDRARAHILEGLKICIENIDAVIKIIKESKTDQEAQSKLSQAFNLSEIQTKAIVDMKLGRLTGLAIEKMNEELNEVHERIANYIRILGDHNLLINLIIEELQEIKNAYGDARRSDINWDELSSIDNEDLIPKRDIVITLTNNGYIKRLDIEEYREQRRGGVGVSTTKTYKDDAIQDILVANTHTDLLIFTDLAKVYRIRGHEVPVGTKQSKGIPIVNVIPSIQKNEKVIKILPINEYKQNEYLLTATKKGIVKKTPTILFESINRNGKIAFAIRDDDDKLIDALIVKDEDEIFIAASNNKICRFDSNEIRSMGRMAYGVKGIKLDDVEDNVVSVSSSNQGKYIFSLGKEGYGKLTLAAMFRKTRRGASGVYSLNEEKSGKLIYAATVHGVEDLIVLTSDGVAIRFSLKDVAITGRNTKGVKLVNLKGRTTSIVAATKILDDDLEDNDRKLSENEFKEIESDAKNNLNQKNE" }, "paralog": false, "identity": 0.9848714069591528, "score": 1271.0, "evalue": 0.0, "description": "Internal stop codon at 615014. Nonsense mutation around 615014.", "psc": { "uniref90_id": "UniRef90_D1J8L4", "gene": "gyrA", "product": "DNA gyrase subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_B3PN30", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L4", "EC:5.6.2.2", "UniRef:UniRef50_B3PN30", "COG:COG0188", "COG:L", "KEGG:K02469", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN30" ], "product": "DNA gyrase subunit A" } }, "id": "DFOKDJOJIG_1075", "locus": "GCA013367855_01074" }, { "type": "cds", "sequence": "contig_1", "start": 615023, "stop": 616078, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03989.18" ], "nt": "TTGAGCAGCATAGACAATGAAGATTTGATTCCAAAACGTGATATTGTTATAACATTAACAAATAATGGCTATATTAAAAGACTTGATATTGAGGAATATAGAGAACAAAGAAGAGGCGGAGTTGGTGTATCAACTACAAAAACATATAAAGATGATGCAATTCAAGATATATTAGTTGCAAATACCCACACGGACTTGTTAATATTTACAGATCTTGCCAAGGTTTATAGAATTAGAGGTCACGAAGTTCCTGTTGGCACCAAACAATCAAAGGGTATTCCTATTGTTAATGTAATTCCTTCTATTCAAAAAAATGAAAAAGTTATTAAAATTTTGCCAATAAATGAATACAAACAAAATGAATATTTACTAACAGCAACTAAGAAGGGGATTGTTAAAAAAACTCCAACAATTTTATTTGAAAGTATTAACAGAAACGGAAAAATTGCCTTTGCCATTCGTGATGATGATGACAAATTAATTGATGCATTGATTGTTAAAGATGAGGATGAAATCTTTATTGCTGCCTCAAATAATAAAATTTGCCGTTTTGATTCAAACGAAATCAGGTCAATGGGAAGAATGGCCTATGGTGTTAAAGGAATTAAAATTGACGATGTTGAAGACAATGTTGTTTCTGTATCTTCTTCAAACCAAGGAAAATATATATTTAGTCTTGGAAAAGAAGGATATGGAAAATTGACACTTGCTGCAATGTTTAGAAAAACAAGGCGTGGAGCAAGCGGAGTTTATTCATTAAATGAAGAAAAATCTGGTAAATTAATTTATGCTGCAACTGTTCACGGAGTTGAAGATCTGATAGTTTTAACAAGCGATGGTGTTGCAATTAGATTTTCTTTAAAAGATGTTGCAATAACCGGTAGAAATACTAAAGGTGTTAAATTAGTAAATCTAAAAGGAAGAACCACAAGCATTGTTGCGGCAACAAAAATTTTAGATGATGATTTAGAAGACAATGACAGAAAATTATCAGAAAATGAATTCAAAGAAATTGAATCAGATGCTAAAAATAACCTTAATCAAAAAAATGAATAA", "aa": "MSSIDNEDLIPKRDIVITLTNNGYIKRLDIEEYREQRRGGVGVSTTKTYKDDAIQDILVANTHTDLLIFTDLAKVYRIRGHEVPVGTKQSKGIPIVNVIPSIQKNEKVIKILPINEYKQNEYLLTATKKGIVKKTPTILFESINRNGKIAFAIRDDDDKLIDALIVKDEDEIFIAASNNKICRFDSNEIRSMGRMAYGVKGIKIDDVEDNVVSVSSSNQGKYIFSLGKEGYGKLTLAAMFRKTRRGASGVYSLNEEKSGKLIYAATVHGVEDLIVLTSDGVAIRFSLKDVAITGRNTKGVKLVNLKGRTTSIVAATKILDDDLEDNDRKLSENEFKEIESDAKNNLNQKNE", "aa_hexdigest": "55d88bc43c480ce905e50f0f43fba182", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03989.18", "name": "DNA_gyraseA_C", "length": 48, "aa_cov": 0.13675213675213677, "hmm_cov": 1.0, "evalue": 5.617115311692782e-57, "score": 184.67364501953125, "start": 14, "stop": 61 } ], "seq_stats": { "molecular_weight": 39147.20080000002, "isoelectric_point": 8.587204933166507 }, "id": "DFOKDJOJIG_1076", "locus": "GCA013367855_01075" }, { "type": "cds", "sequence": "contig_1", "start": 616452, "stop": 616619, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAACTTTAAAGAACACAAAGCCACAACAGCGACCCTTAAACGTGGATCTTATTCTATACCATATACCAGAATTGATCCAAATAAATCAATATACAAAGAAGTTAATGATACTATAAAACGCTTAGAACAAAGTGGAAAATATGCCTCAAAAATTTCTGAATGA", "aa": "MSNFKEHKATTATLKRGSYSIPYTRIDPNKSIYKEVNDTIKRLEQSGKYASKISE", "aa_hexdigest": "061850484c6331e0555212d51b0fa3fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6325.082000000002, "isoelectric_point": 9.696899223327637 }, "id": "DFOKDJOJIG_1077", "locus": "GCA013367855_01076" }, { "type": "cds", "sequence": "contig_1", "start": 616920, "stop": 617255, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTAATTAATGAAATTTCAGTGGAATATCTTGAGGCTAAAAAATTATTAATAAATAATAGTCTAGATGATATAAATAATAGTTTGCAAAAATTTAATAAGCTAGATAGTAGTGTAAAAGATCCAGATTTTTTAGGTGTAACACAACAACTAAATGAAATAAAACAAAAGGCATTGCAAGAATTAGCAAATATCAAAAATAATTCTAGTCAATTGGCAACTCTTGCAGAAGCGTCAAAATATGAAAATGATATACAAGAAATCCAAGAAAAATTTGTTAAAGATTTAATAAAAATATTTGATGGAAATATAAATACTGATAGGATTAATTGA", "aa": "MQVINEISVEYLEAKKLLINNSLDDINNSLQKFNKLDSSVKDPDFLGVTQQLNEIKQKALQELANIKNNSSQLATLAEASKYENDIQEIQEKFVKDLIKIFDGNINTDRIN", "aa_hexdigest": "bfdab63707ab8c5a8eb7a39ecc41d237", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12669.128299999986, "isoelectric_point": 4.693388938903809 }, "id": "DFOKDJOJIG_1078", "locus": "GCA013367855_01077" }, { "type": "cds", "sequence": "contig_1", "start": 617304, "stop": 618236, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F519C31", "PFAM:PF04778.17" ], "nt": "ATGAACAATAAAAGCAAAAAGGTCGTTCTTCCATTAGCAATTTTTTGTGGCGGATTATCAATAGCTGCCATCGCTACCGTTGCAATAAGATCGTGTCAAAATAAAGTGGATGAAAAGACACAAAAAGAATCACTCTTTAAAAAATTTAAAATTCTTCAAGAAGAATTGCAATTATTGCTAGAAAATAAAAACATTCAAGGAATTTATTCAATTGAAGAACAAAATGTCTTTGATAATTCCAAAATGATCAATATCGAATCGCCAATTGATAATCTTAATCAAGAAATTTTTAAGATTAAAAGTTCGATTGATTCATTAAATGCAAAAATTCAAAAGCAAAGCATGCAAAAACTACTTTTTGCAGCAAAGAAACAGTTGGAAAATATTATTCATGTTATGAGGATATTGGAAAATAAATTAATTGATAATGATGATGATATTCAAAAAACTAAGGAAGATTTAAATTCACAAATTATTAATGCATTAGAGGTTTATAACAAAAATAATATAGACGAAATAAATAATTTAAATAAATCATTAACTTTTAAAATTTCTGAAGTTGAAGATATATTGATCAAATTTAAAAAATCAAAAGAACAAGAATTTGAAAAATTACAAAAAACTAGAAAATTAGTTGAAGAATTTTTGGCCAAAGATGTTGTTAAATCTGATTATTATACAGATATAGTTAGAAATCTTAATGGAGAAGTTGCAAGACAAGCAAACGTTTCAATTAGTTCTAACAAATCAGAAATAGTAGCTACAAATATTCATTTGAACGAAATTTTAAAACAAAGTAAAAAATTTGTTGAAGATGATATGCAAAAATTAAGGGATAGATTAATTTCTTTAATTATAGAAGCAAAAGAATATATTGAAAATAAAATGCGCCCTAGAGATAAATACAATGCATACATTGAAAGAATAGAATGA", "aa": "MNNKSKKVVLPLAIFCGGLSIAAIATVAIRSCQNKVDEKTQKESLFKKFKILQEELQLLLENKNIQGIYSIEEQNVFDNSKMINIESPIDNLNQEIFKIKSSIDSLNAKIQKQSMQKLLFAAKKQLENIIHVMRILENKLIDNDDDIQKTKEDLNSQIINALEVYNKNNIDEINNLNKSLTFKISEVEDILIKFKKSKEQEFEKLQKTRKLVEEFLAKDVVKSDYYTDIVRNLNGEVARQANVSISSNKSEIVATNIHLNEILKQSKKFVEDDMQKLRDRLISLIIEAKEYIENKMRPRDKYNAYIERIE", "aa_hexdigest": "ce972d6435e46a9acad19d1289ce1fa7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001F519C31", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.897, "score": 505.0, "evalue": 3.9e-179, "valid": false, "uniref50_id": "UniRef50_UPI001F519C31" }, "pscc": { "uniref50_id": "UniRef50_UPI001F519C31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F519C31" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 154, "aa_cov": 0.4870967741935484, "hmm_cov": 1.0, "evalue": 1.1184962252541509e-51, "score": 170.50457763671875, "start": 135, "stop": 285 } ], "seq_stats": { "molecular_weight": 36028.158000000025, "isoelectric_point": 8.519771003723147 }, "id": "DFOKDJOJIG_1079", "locus": "GCA013367855_01078" }, { "type": "cds", "sequence": "contig_1", "start": 618640, "stop": 618849, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAACACAACCAATAAAATGCTAAATAAAAGTACAACATTGCTTTTTACAGAAAATTTTCTATTTTTTATAAATAAAGTTAAAAAGGTTATAGAAGATATTCAAATAAATGCTAATCCCACAATAGCAATAGATAAAACAATTCTGATTGGTATTAATTTGGTGCGTTTTACTGCTGTTTCATTTATTGGCAAATTGTTACCGTGA", "aa": "MKNTTNKMLNKSTTLLFTENFLFFINKVKKVIEDIQINANPTIAIDKTILIGINLVRFTAVSFIGKLLP", "aa_hexdigest": "f97a2cba848694ae3003f47691d11ce4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7812.2802999999985, "isoelectric_point": 9.998095512390137 }, "id": "DFOKDJOJIG_1080", "locus": "GCA013367855_01079" }, { "type": "cds", "sequence": "contig_1", "start": 618883, "stop": 619107, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAAAACTAAATCCAATTATTCATGTAATCTTTTGTTTAGCATACATTAATCTTAATAATCAACCACTTATTAAAGCAATAAAAACAGGCACTAATGCATATTCAATCATTCATGTGCTTATTCCTCTAATAACCTGGTTTAGCGTATCACATAATATACCTAAAAATGCACCCTTTCATGGTCCCAATGCCAAGCCAAAAATAATGAATACTGCATAA", "aa": "MLKKLNPIIHVIFCLAYINLNNQPLIKAIKTGTNAYSIIHVLIPLITWFSVSHNIPKNAPFHGPNAKPKIMNTA", "aa_hexdigest": "531c74749d2b600841ead478197e7d55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8271.855099999993, "isoelectric_point": 10.111173057556147 }, "id": "DFOKDJOJIG_1081", "locus": "GCA013367855_01080" }, { "type": "cds", "sequence": "contig_1", "start": 619065, "stop": 619268, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAATTTATTCAAAAAATTAGACGTATTAAAAAAACAAAACAACATAAATTAACAGTTTTTGAAATTTCTTTATTTGCTTTACTTTTAGCTATTTATATAATTGCTGCAATCTTAGAAAGATTTGTTTTTAAAGGAATAATGAATATTAATATTACTTATGCAGTATTCATTATTTTTGGCTTGGCATTGGGACCATGA", "aa": "MFEFIQKIRRIKKTKQHKLTVFEISLFALLLAIYIIAAILERFVFKGIMNINITYAVFIIFGLALGP", "aa_hexdigest": "8d8277910469a4365480cd7504f96ef4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7789.507999999994, "isoelectric_point": 10.21090564727783 }, "id": "DFOKDJOJIG_1082", "locus": "GCA013367855_01081" }, { "type": "cds", "sequence": "contig_1", "start": 619443, "stop": 619670, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCTTTTGATTCTGACTATGAAGAATTTATGAAGGGTTCAATTGTTGCAGATTTGAAGAATACAGACTATACTGGACATGCAGGTTCTTGTTCAGCAGCAATGTTCTTAAAAGAATTTACAAAGGGAATTGAATATATTCATTTAGATGTTGCAGGAACTAATGATATTGATGAAAAACCAATGTTTGCAATGACAAAAACTTTGACAGAATTAGTGTTGAAATAA", "aa": "MPFDSDYEEFMKGSIVADLKNTDYTGHAGSCSAAMFLKEFTKGIEYIHLDVAGTNDIDEKPMFAMTKTLTELVLK", "aa_hexdigest": "d7f4f7412216ed7607c491ffc4bc2a8d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 59, "aa_cov": 0.7866666666666666, "hmm_cov": 0.9491525423728814, "evalue": 8.961892642183399e-15, "score": 50.34300994873047, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 8373.472299999996, "isoelectric_point": 4.60369701385498 }, "id": "DFOKDJOJIG_1083", "locus": "GCA013367855_01082" }, { "type": "cds", "sequence": "contig_1", "start": 619740, "stop": 620357, "strand": "-", "frame": 2, "gene": null, "product": "M17 family metallopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201A65A" ], "nt": "TTGGGTATTACCCCTCCTAATGAATTAAATTCTGAACAATTAGCTAAAATAGTTGTTGAAGACTTTAAAGAATATAAAAACCTATCAGTTAAAGTTCTTGAAAAGAAAGAAATTGAAAAATTGGGTATGGGATTATTACTATCTGTGAATAGAGGTTCAGTTTATGAACCTAGAGTTGTAATAATTGAATATAAAGGAAATCCTGATTCAAATCAAAAAACAGTTTTAATTGGTAAAGGAATTACCTTTGACTCTGGCGGTTACAACATTAAAACAGGCCGCAACATGTTAGGAATGAAATATGACATGTCAGGTTCTGCAATAGTTGCATCCGTTTTGAAATCTGTTGCCCAATTAAAACCAAAAACAAATGTATCAGCAATTATGTGCATTACTGACAATAGAGTTAATGGAGATGCATCGTTGCCTGATTCAGTTTGGACCTCAATGAGCGGAAAGACTGTTGAAATAAATAATACTGACGCTGAAGGTAGATTAGTTATGGCTGATGGCTTGTACTATGGAGCTGATGTATTAAAAGCAACAAGATTAATTGATGTTGCCACATTAACTGGTGCAATGGTTGCAGCCCTAGGAGACACATATACAGGCATTTGA", "aa": "MGITPPNELNSEQLAKIVVEDFKEYKNLSVKVLEKKEIEKLGMGLLLSVNRGSVYEPRVVIIEYKGNPDSNQKTVLIGKGITFDSGGYNIKTGRNMLGMKYDMSGSAIVASVLKSVAQLKPKTNVSAIMCITDNRVNGDASLPDSVWTSMSGKTVEINNTDAEGRLVMADGLYYGADVLKATRLIDVATLTGAMVAALGDTYTGI", "aa_hexdigest": "3394784bffc5a402b5bc81c8a5fdddbf", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002201A65A", "query_cov": 0.9365853658536586, "subject_cov": 0.8170212765957446, "identity": 0.594, "score": 222.0, "evalue": 2.96e-70, "valid": false, "product": "M17 family metallopeptidase", "uniref50_id": "UniRef50_UPI002201A65A" }, "pscc": { "uniref50_id": "UniRef50_UPI002201A65A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201A65A" ], "product": "M17 family metallopeptidase" }, "genes": [], "id": "DFOKDJOJIG_1084", "locus": "GCA013367855_01083" }, { "type": "cds", "sequence": "contig_1", "start": 620367, "stop": 620816, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAATTAAACAATTATCAACAAAGCGCAATGATCATTTGTTATTGAAAGCAATATTTGAAGATGAAATTTTGCCAGAATTTTTAGTTAAAGAAACAGAAGCAATAACAGATTTTATTGATAAAAAAGTTTCGTATATTTATCTAGGCAAAAAACAAGAATTACAATACAATTCTTTATATTCATTGGCACAAGATTTAGCATTGAATGCTGCCTACGATTATCAAATTGATCTTTCTTCTTTTGTAACAGAAAATTTAACAATTAATGATGTAGTTGATGCATTAACAAAAGGAATAAATTTCTCAGCTGCAAAGTTATACAATAAAAAGACATTTACATATAAAGAAAATCCAAACAACATAGAATTGTTTATAGAACAAGCGTCTCAATCAGTACTTGACTCATTCAATAAAGCAAAAATATTAATTGAATCTCAAAACTGA", "aa": "MQLIKQLSTKRNDHLLLKAIFEDEILPEFLVKETEAITDFIDKKVSYIYLGKKQELQYNSLYSLAQDLALNAAYDYQIDLSSFVTENLTINDVVDALTKGINFSAAKLYNKKTFTYKENPNNIELFIEQASQSVLDSFNKAKILIESQN", "aa_hexdigest": "3c9b75026abbeff3a1f541b35e1c095d", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17127.276999999984, "isoelectric_point": 4.910002326965332 }, "id": "DFOKDJOJIG_1085", "locus": "GCA013367855_01084" }, { "type": "cds", "sequence": "contig_1", "start": 620830, "stop": 621063, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCTCTACATGATGATTATGATAAACCAAATAAAGAATCTAAAGTAGCTGATCTTAATAATTACAACAACAGTGAAAAATCAGATTGTAATACAGCAGCAATGTTCTTAAAACATTTTACAAATAATGTTGACTATATTCATTGCGATATTGCAGGAACCGCTGACAATAAAGGAGTAGGACTAGGAATTTTAGTTTCTACCTTTGTTGAATTAGCAGCAAATCAAAAATAA", "aa": "MPLHDDYDKPNKESKVADLNNYNNSEKSDCNTAAMFLKHFTNNVDYIHCDIAGTADNKGVGLGILVSTFVELAANQK", "aa_hexdigest": "fe1abfe2770c91cf8972a0375caca55b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 58, "aa_cov": 0.7532467532467533, "hmm_cov": 0.9655172413793104, "evalue": 5.642695213255274e-15, "score": 51.002933502197266, "start": 1, "stop": 58 } ], "seq_stats": { "molecular_weight": 8490.333599999998, "isoelectric_point": 5.05994358062744 }, "id": "DFOKDJOJIG_1086", "locus": "GCA013367855_01085" }, { "type": "cds", "sequence": "contig_1", "start": 621133, "stop": 622194, "strand": "-", "frame": 1, "gene": "pepB", "product": "Aminopeptidase (Leucine aminopeptidase)", "db_xrefs": [ "COG:COG0260", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZXA3", "UniRef:UniRef90_D1J8L9" ], "nt": "ATGAATTCTATTTATTATGAAACTAAACGTAATAAAGATATGCTTTTAACAGCAGTTTATGAAGGCTCAAACGCTAGTTTAAATATTGCCAAGAAAGATAATCATATTACAGAAGATTTTAAAAAAAATGAAGCATTTATTTACATACCAAAAACTGTAGATAACTACTTTAGTTTTTTAAAAGTAGTTGACAAAATTCTAAGTTCAAATAGAAGAAATTATCAAATTAATATTGCTTCTTTTGTTAAAGAAAATGTTGTGTCAACAGAAGAAGTTTTTAGAGCATTTGTATCTAAATTAGCATTTAAAAATGCAAATCTTTATTCAGCAAAAACTAAAGAAAACAAACAAGAAAAGACAATAACATTGTTTTATGCTGATAAATCGTATGAAGAATTAGTTGAAAGATTGTCAATAATTCTTGAAGCAACAAATAAAGCCCGCAGTTTGCAAATGACACCCCCTAATGTCGCAACTAGTGAATATATCGCTAAGGTAGTAAAAGAAGATTTAGAAAATATTCCTGGACTATCAATAAAAGTTTTAGATCGTGATAACATCAAGAAAAATAACATGGGACTTATGTTGGCAGTAAATTCGGGTTCTTCTTATGAACCAAGAGTTTTAATTGCTGAATATAATGGCAATCCTGAAAGTAAAGAAAAATATGTTTATGTAGGAAAAGGAATTACATTTGATACCGGAGGATATAACACCAAAGGATACTACATGGATGGCATGAAATTTGACATGTCTGGTTCTGTTATTGTTGCATATGCAGTTAAAGCAATTGCTCAACTAAAATTAAAAGCAAACGTAGCAGCAATAATGATGATTACAGATAACGCAATAGATACACACCCAACAATGCCTGAATCTGTAATCAAATCAATGAGTGGTTTAACAGTTGAAATTACTGATACTGATGCTGAAGGAAGATTAGTGTTGGCTGATGGTTTATACTACGGTGCCTCGAAATTGAATGCTTCATTATTGATTGATGCCGCAACTTTAACCGGTTCGATGGAAAGAGCATTAGGAAAGACGTATTCAGGAATTTGA", "aa": "MNSIYYETKRNKDMLLTAVYEGSNASLNIAKKDNHITEDFKKNEAFIYIPKTVDNYFSFLKVVDKILSSNRRNYQINIASFVKENVVSTEEVFRAFVSKLAFKNANLYSAKTKENKQEKTITLFYADKSYEELVERLSIILEATNKARSLQMTPPNVATSEYIAKVVKEDLENIPGLSIKVLDRDNIKKNNMGLMLAVNSGSSYEPRVLIAEYNGNPESKEKYVYVGKGITFDTGGYNTKGYYMDGMKFDMSGSVIVAYAVKAIAQLKLKANVAAIMMITDNAIDTHPTMPESVIKSMSGLTVEITDTDAEGRLVLADGLYYGASKLNASLLIDAATLTGSMERALGKTYSGI", "aa_hexdigest": "c2220d84e2261c350621b4af6a4940d3", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "pepB" ], "truncated": "5-prime", "pseudogene": { "start": 620830, "stop": 622194, "observations": { "mutation": [ 621069, 621114, 621135 ], "stop": [ 621069, 621114, 621135 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L9", "query_cov": 1.0, "subject_cov": 0.7775330396475771, "identity": 1.0, "score": 674.0, "evalue": 8.34e-243, "gene_start": 1, "gene_end": 353, "reference_start": 1, "reference_end": 353, "reference_sequence": "MNSIYYETKRNKDMLLTAVYEGSNASLNIAKKDNHITEDFKKNEAFIYIPKTVDNYFSFLKVVDKILSSNRRNYQINIASFVKENVVSTEEVFRAFVSKLAFKNANLYSAKTKENKQEKTITLFYADKSYEELVERLSIILEATNKARSLQMTPPNVATSEYIAKVVKEDLENIPGLSIKVLDRDNIKKNNMGLMLAVNSGSSYEPRVLIAEYNGNPESKEKYVYVGKGITFDTGGYNTKGYYMDGMKFDMSGSVIVAYAVKAIAQLKLKANVAAIMMITDNAIDTHPTMPESVIKSMSGLTVEITDTDAEGRLVLADGLYYGASKLNASLLIDAATLTGSMERALGKTYSGIWSTSEQRWEDFNKAAKIAHEKVWRMPLHDDYDKPNKESKVADLNNYNNSEKSDCNTAAMFLKHFTNNVDYIHCDIAGTADNKGVGLGILVSTFVELAANQK" }, "paralog": false, "identity": 0.9933920704845814, "score": 878.0, "evalue": 0.0, "description": "Internal stop codon at 621069, 621114, 621135. Nonsense mutation around 621069, 621114, 621135.", "psc": { "uniref90_id": "UniRef90_D1J8L9", "gene": "PepB", "product": "Aminopeptidase (Leucine aminopeptidase)", "uniref50_id": "UniRef50_A0A448ZXA3", "cog_id": "COG0260", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L9", "UniRef:UniRef50_A0A448ZXA3", "COG:COG0260", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXA3" ], "product": "Cytosol aminopeptidase" } }, "id": "DFOKDJOJIG_1087", "locus": "GCA013367855_01086" }, { "type": "cds", "sequence": "contig_1", "start": 622355, "stop": 622645, "strand": "-", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_041359639.1", "SO:0001217", "UniParc:UPI0002D31643", "UniRef:UniRef100_A0A6A8Q1U1", "UniRef:UniRef50_A0A454CA46", "UniRef:UniRef90_A0A454CA46" ], "nt": "ATGAAGAAGAGTAAAAAAATAATTTTGTCTATTTTAGGAGCAAGTTCTTTAATTTCATTACCAATAGTAGCAATATCGTGTTCAAAATTGACATCAAAGCAACAACAAGTTAAATCAAAAATAAAGCAATTACTTGATATGGCAAAAAAGATACCATCTGCTGGTGAAATTATGAACCAATATGAAAAAATGATAAAAGATGAAGAAATTGCAAAACTAAATGATCAACAATGCGACGCAATCTTATTTAGCATAGATTTAGTTATTAATCAACTTAAAAATATTAAATAA", "aa": "MKKSKKIILSILGASSLISLPIVAISCSKLTSKQQQVKSKIKQLLDMAKKIPSAGEIMNQYEKMIKDEEIAKLNDQQCDAILFSIDLVINQLKNIK", "aa_hexdigest": "e2c6b7c186694b9f6f55ddb0ae936042", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0002D31643", "ncbi_nrp_id": "WP_041359639.1", "uniref100_id": "UniRef100_A0A6A8Q1U1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D31643", "RefSeq:WP_041359639.1", "UniRef:UniRef100_A0A6A8Q1U1" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8Q1U1", "uniref90_id": "UniRef90_A0A454CA46" }, "psc": { "uniref90_id": "UniRef90_A0A454CA46", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_A0A454CA46" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA46" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "DFOKDJOJIG_1088", "locus": "GCA013367855_01087" }, { "type": "cds", "sequence": "contig_1", "start": 622723, "stop": 622854, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAAACAAATAATAGTTTATGCGCCACATCCTTCACCTCTTGCTGCATATAAAGGATTTTTTGGTTCAAATGTTTTTAAAAAAATAAATTTTAAACTTAAATCATTATCAAAAGATGAAATCGATTGA", "aa": "MSKQIIVYAPHPSPLAAYKGFFGSNVFKKINFKLKSLSKDEID", "aa_hexdigest": "6572a11bb18712a3dec1c80bc831498f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4858.655100000001, "isoelectric_point": 9.6994779586792 }, "id": "DFOKDJOJIG_1089", "locus": "GCA013367855_01088" }, { "type": "cds", "sequence": "contig_1", "start": 623041, "stop": 623358, "strand": "-", "frame": 1, "gene": "ung", "product": "uracil-DNA glycosylase", "db_xrefs": [ "COG:COG0692", "COG:L", "EC:3.2.2.27", "GO:0004844", "GO:0006284", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_D1J8M1", "UniRef:UniRef90_D1J8M1" ], "nt": "ATGTCAAAATTTGCTTTTTTAGAATTTTTAAAAAAAGAAACTTCAAAACCATATTTTGAAGTCTTAAAAAAATTAACTATGTTGCCTAATGTAACGCCAAAGCCAAACAGAGTATTTTTTGCATTTAAAAATTTCGATTTTGACAACCTAAAGGTTATTATTATTGGCCAAGATCCTTATCCCAACAAAGGTGACGCAGATGGTATGTGTTTTTCAAGCAATGCAACAAAATGCCCGGCATCTTTAAAAAATATATTTATTGAAATAAAAAATGAATATCCAAATTCAACATTTGAAACTTTTTCGCTAAATTATTGA", "aa": "MSKFAFLEFLKKETSKPYFEVLKKLTMLPNVTPKPNRVFFAFKNFDFDNLKVIIIGQDPYPNKGDADGMCFSSNATKCPASLKNIFIEIKNEYPNSTFETFSLNY", "aa_hexdigest": "6462a89db03abe20018c647a81e71307", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ung" ], "truncated": "5-prime", "pseudogene": { "start": 622738, "stop": 623358, "observations": { "mutation": [ 622962, 623043 ], "stop": [ 622962, 623043 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M1", "query_cov": 1.0, "subject_cov": 0.4861111111111111, "identity": 0.99, "score": 215.0, "evalue": 2.38e-69, "gene_start": 1, "gene_end": 105, "reference_start": 1, "reference_end": 105, "reference_sequence": "MSKFVFLEFLKKETSKPYFEVLKKLTMLPNVTPKPNRVFFAFKNFDFDNLKVIIIGQDPYPNKGDADGMCFSSNATKCPASLKNIFIEIKNEYPNSTFETFSLNYWKNQGVLLLNSILTNIEGKTLAHKNLGWELFNQNLLTEINSCYSNIIYLILGNNAKDFIKNIDMSKQIIVYAPHPSPLAAYKGFFGSDVFKKINFKLKSLSKDGIDWSTKK" }, "paralog": false, "identity": 0.9757281553398058, "score": 405.0, "evalue": 8.53e-147, "description": "Internal stop codon at 622962, 623043. Nonsense mutation around 622962, 623043.", "psc": { "uniref90_id": "UniRef90_D1J8M1", "gene": "Ung", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_D1J8M1", "cog_id": "COG0692", "cog_category": "L", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0006284" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M1", "EC:3.2.2.27", "UniRef:UniRef50_D1J8M1", "COG:COG0692", "COG:L", "KEGG:K03648", "GO:0004844", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M1" ], "product": "Uracil-DNA glycosylase" } }, "id": "DFOKDJOJIG_1090", "locus": "GCA013367855_01089" }, { "type": "cds", "sequence": "contig_1", "start": 623361, "stop": 623666, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02911.23" ], "nt": "TTGTTTGAAATTTACAACGGCCAATATAATCGAGTGGCTCAGGATGAATCTAAAGTAACATTTGCTCCTAAGATATTAAATGGTATGGAAAGGATTGATCAAAGCGAAAATCTTTCAAGTGTTGTAAACAAAATCAGAGCTTTTAATGATGAACCAGGTGCATATTTAATTTTTCAAAATAAAAGATTAAAAATCTTCCGGGCTTCAACAACAAAAATTAGTACCCCATTGAAAATAAAATTTAATGATGGATATTTGTACTTAATAGAATATCAATTTGAAGGGAAAAAAAGAGTAAGAGTTTAA", "aa": "MFEIYNGQYNRVAQDESKVTFAPKILNGMERIDQSENLSSVVNKIRAFNDEPGAYLIFQNKRLKIFRASTTKISTPLKIKFNDGYLYLIEYQFEGKKRVRV", "aa_hexdigest": "e6c751612b4fecbc4246db4a139adee5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02911.23", "name": "Formyl_trans_C", "length": 86, "aa_cov": 0.7128712871287128, "hmm_cov": 1.0, "evalue": 1.1230958226911603e-12, "score": 43.66648864746094, "start": 28, "stop": 99 } ], "seq_stats": { "molecular_weight": 11846.478999999987, "isoelectric_point": 9.653898811340333 }, "id": "DFOKDJOJIG_1091", "locus": "GCA013367855_01090" }, { "type": "cds", "sequence": "contig_1", "start": 623667, "stop": 624278, "strand": "-", "frame": 2, "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "db_xrefs": [ "COG:COG0223", "COG:J", "EC:2.1.2.9", "GO:0004479", "GO:0005737", "GO:0071951", "SO:0001217", "UniRef:UniRef50_B3PNE5", "UniRef:UniRef90_D1J8M2" ], "nt": "TTGCTTGAAATACAAGATAATAAGTTTATAAAAAATAATTTATTTTATAATTTTGATATGAAAAATATAAATAACAAAATTAATTTAGTTCTAGCGGGAACTGGACAATTTTCTGCTAAAACTTTTGAACCTCTTTTAAATGATGAAAGGTTCAATGTTTTGGCATTTATTTCTCAGCCAAATAGAAAATTGGATCGAAACAAAAATGTAATTGAAACAGAAATTTTTAAACTTGCCAAACAGTATGATATAAAAGTTTTTCAACCTCAAAAAATAAAAGAAATTTTGCCCGAACTTAGCGCAATGGATTTTGACTTTTTTATCACAGCTTCATTTGGACAATTTATACCTGATTCAATATTAAAATTACCTAAAAAAATGCCCTTGAACGTTCACGGAAGTTTGCTTGAAAAATATCGTGGAGCTGCGCCTGTGCAATATGCATTACTCAATGGTGATATTGAAACAGGAATAACATTAATTGAAATGGTAAAACAAATGGATGCAGGAGATATCCTAGAATCCTCGAAAGTGAAAATTGATTCTACAGATACAGCAATTGAAGTATTTGATAAATTAGCAAATGCGGCCTCGCAAAATCTTGGTAATTGA", "aa": "MLEIQDNKFIKNNLFYNFDMKNINNKINLVLAGTGQFSAKTFEPLLNDERFNVLAFISQPNRKLDRNKNVIETEIFKLAKQYDIKVFQPQKIKEILPELSAMDFDFFITASFGQFIPDSILKLPKKMPLNVHGSLLEKYRGAAPVQYALLNGDIETGITLIEMVKQMDAGDILESSKVKIDSTDTAIEVFDKLANAASQNLGN", "aa_hexdigest": "c21f718161a4d8369727ba02f64e17ab", "start_type": "TTG", "rbs_motif": null, "genes": [ "fmt" ], "truncated": "5-prime", "pseudogene": { "start": 623364, "stop": 624278, "observations": { "mutation": [ 623669 ], "stop": [ 623669 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M2", "query_cov": 1.0, "subject_cov": 0.6655737704918033, "identity": 0.975, "score": 384.0, "evalue": 2.91e-133, "gene_start": 1, "gene_end": 203, "reference_start": 1, "reference_end": 203, "reference_sequence": "MLEIQDNKFIKNNLFYNFDMKNINNKINLVLAGTGQFSAKTFEPLLNDERFNVLAFISQPNRKLDRNKNVIETEIFKLAKQYDIKVFQPQKIKEILPELSAMDFDFFITASFGQFIPDSILKLPKKMPLNVHGSLLEKYRGAAPVQYALLNGDIETGITLIEMVKQMDAGDILKSSKVKIDSIDTAIEVFDKLADVASKNLGNWLFEIYNGQYNRVAQDESKVTFAPKILNGMERIDQSENLSSVVNKIRAFNDEPGAYLIYQNKRLKIFRASTTKISTPLKIKFNDGYLYLIEYQFEGKKRVRV" }, "paralog": false, "identity": 0.9736842105263158, "score": 582.0, "evalue": 1.25e-213, "description": "Internal stop codon at 623669. Nonsense mutation around 623669.", "psc": { "uniref90_id": "UniRef90_D1J8M2", "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "ec_ids": [ "2.1.2.9" ], "uniref50_id": "UniRef50_B3PNE5", "cog_id": "COG0223", "cog_category": "J", "go_ids": [ "GO:0004479", "GO:0005737", "GO:0071951" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M2", "EC:2.1.2.9", "UniRef:UniRef50_B3PNE5", "COG:COG0223", "COG:J", "GO:0004479", "GO:0005737", "GO:0071951" ] }, "pscc": { "uniref50_id": "UniRef50_B3PNE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PNE5" ], "product": "Methionyl-tRNA formyltransferase" } }, "id": "DFOKDJOJIG_1092", "locus": "GCA013367855_01091" }, { "type": "cds", "sequence": "contig_1", "start": 624319, "stop": 624717, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGCAAATAGCAAAGATCTAATTGCAAGGTTAACAATTTTGAAATTCTTGAATCAACAAAAAGATTTATTTCTAGCAAATAATAATGCATTGCCAGCCAATACACCAATTGGAATAAAATATAGTCAATATAATATATTTAATATTAACAACGAATATAAAACACTAAATATTTTAAATGTTTATCAACCTATAATTTTAAAATACTCAACCATTGCTGAAACATTCAATCAAAATGGTTTGGACCAATTGTTAAAGAATAATGAATTTGCAAACTATTTTAAATTTTTAGACAGTAGTTACATTCAATTTTCAAATAACGTTTTAAGCAATGATAGTTTAACAAAAATAGGTTCAAAAACAGAAAATTTATTGAACTTCATATTGCCTTGA", "aa": "MKEANSKDLIARLTILKFLNQQKDLFLANNNALPANTPIGIKYSQYNIFNINNEYKTLNILNVYQPIILKYSTIAETFNQNGLDQLLKNNEFANYFKFLDSSYIQFSNNVLSNDSLTKIGSKTENLLNFILP", "aa_hexdigest": "d7c40f680cf749252d7b96cc8861e49e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15178.125699999986, "isoelectric_point": 8.776935386657716 }, "id": "DFOKDJOJIG_1093", "locus": "GCA013367855_01092" }, { "type": "cds", "sequence": "contig_1", "start": 624961, "stop": 625293, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGTTGCCTTAACGTTTTTTTGCGAATATTTTAAAACCGGTCGTTCTTTAGGAACAATTAATAATATAAAAATTGGAGAAGCAAGTCTAAATATAACTTTATATGGAAGTAGTATTAAAGAAATGTTCTCAAGGATATTTTGGCCAGGTTATATATTTAGTTTTGTTATTATAATTTTTGTAAGCATATCTGTTGGATTGTTCATTATAAGCGTTTCAAAATCAGTATTAACTATTCAAGCAATAGGTTGTTCGCTATTAATAATCACAATGTTCTTATCGGGCCAAGTGTTGCCTATTTCTCAAATTTCAAATATAAAAGGAATGTGA", "aa": "MLVALTFFCEYFKTGRSLGTINNIKIGEASLNITLYGSSIKEMFSRIFWPGYIFSFVIIIFVSISVGLFIISVSKSVLTIQAIGCSLLIITMFLSGQVLPISQISNIKGM", "aa_hexdigest": "0a865966877a13a623249776a2ee8c91", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12113.436799999987, "isoelectric_point": 9.390352058410645 }, "id": "DFOKDJOJIG_1094", "locus": "GCA013367855_01093" }, { "type": "cds", "sequence": "contig_1", "start": 625367, "stop": 625492, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGAAGCACCAATTCTTTTTAAAATTGTTGATTTTTTAAACTCAAATATTGCCGTTGGCAGTGAAATACAGGCTATTGCCACCGGCCCAATGGTAATTGTACTTCCTAAAATCATTTCATAA", "aa": "MLEAPILFKIVDFLNSNIAVGSEIQAIATGPMVIVLPKIIS", "aa_hexdigest": "365c3ba57a6f825defea4261b7090197", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4367.260099999999, "isoelectric_point": 4.677076148986816 }, "id": "DFOKDJOJIG_1095", "locus": "GCA013367855_01094" }, { "type": "cds", "sequence": "contig_1", "start": 625567, "stop": 625662, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAAGAAATTATTAAAAAAAACAATAATACCGAGGCTAACAAAAATAAGTTTTTAGGAATATTAGCAATTATTAATAAACATTTTTGA", "aa": "MKNKEIIKKNNNTEANKNKFLGILAIINKHF", "aa_hexdigest": "5e2333114520e6db4adb5499d072849b", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3627.2647000000006, "isoelectric_point": 10.12516269683838 }, "id": "DFOKDJOJIG_1096", "locus": "GCA013367855_01095" }, { "type": "cds", "sequence": "contig_1", "start": 625667, "stop": 626077, "strand": "-", "frame": 3, "gene": "rbbA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1131", "COG:JV", "SO:0001217", "UniRef:UniRef50_D1J8M4", "UniRef:UniRef90_D1J8M4" ], "nt": "ATGAACAAAATCTATGGCAATAAAAGTTCACCAGAAGAATTGCAAAAACTTATAAAGATTTTTGGGGTTGATGAATTTATAAATAATAAAGCTTCTTCCTTAAGCGGGGGCCAACATCAAAGACTTAATGCATTGCTTGCAATAATTAATAAACCAAAGTTGTTAATATTAGATGAATTAAGCACTGGTCTTGACATAAAAATAAAAACAAGATTGGTATCATTTTTGAATGACTATATCAAAGAAATTGATTGTACTTTGATTTTAATATCTCACGATATAAATGAAATTGAAATTCTGGCAGATAGAATTATTATTATGAATAAAGGTAGAATTGTCCTTGATGCTTTGAAAAGCGAAATAATTGAAAAATATGGATCAATATCTCAATGTCTTAAGGAATATATTTAA", "aa": "MNKIYGNKSSPEELQKLIKIFGVDEFINNKASSLSGGQHQRLNALLAIINKPKLLILDELSTGLDIKIKTRLVSFLNDYIKEIDCTLILISHDINEIEILADRIIIMNKGRIVLDALKSEIIEKYGSISQCLKEYI", "aa_hexdigest": "27f37d3670ac3fe0f0d6f3a983cafa38", "start_type": "ATG", "rbs_motif": null, "genes": [ "rbbA" ], "truncated": "3-prime", "pseudogene": { "start": 625667, "stop": 626377, "observations": { "mutation": [ 626176 ], "stop": [ 626176 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M4", "query_cov": 1.0, "subject_cov": 0.48226950354609927, "identity": 1.0, "score": 256.0, "evalue": 3.88e-84, "gene_start": 1, "gene_end": 136, "reference_start": 147, "reference_end": 282, "reference_sequence": "MENEKVCKNKSLEIIDNFLSNLEDHEKNTIKANSIEMRQYNLKKLVKIKNLTKEFKIKKEKIKVVDNLNLEIYKNQNIALLGGNGAGKTTTVEMLIGILKPTSGNIEYYFNDGWSNHNESAQTNIGIQFQDSSYPQGLSVNDVIYSMNKIYGNKSSPEELQKLIKIFGVDEFINNKASSLSGGQHQRLNALLAIINKPKLLILDELSTGLDIKIKTRLVSFLNDYIKEIDCTLILISHDINEIEILADRIIIMNKGRIVLDALKSEIIEKYGSISQCLKEYI" }, "paralog": false, "identity": 0.9957627118644068, "score": 456.0, "evalue": 2.45e-165, "description": "Internal stop codon at 626176. Nonsense mutation around 626176.", "psc": { "uniref90_id": "UniRef90_D1J8M4", "gene": "RbbA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_D1J8M4", "cog_id": "COG1131", "cog_category": "JV", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M4", "UniRef:UniRef50_D1J8M4", "COG:COG1131", "COG:JV" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M4" ], "product": "ABC transporter ATP-binding protein" } }, "id": "DFOKDJOJIG_1097", "locus": "GCA013367855_01096" }, { "type": "cds", "sequence": "contig_1", "start": 626174, "stop": 626515, "strand": "-", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A5ABD3", "UniRef:UniRef90_UPI0034A5ABD3" ], "nt": "GTGGAAAATGAAAAAGTTTGTAAAAATAAAAGTCTAGAAATAATAGACAATTTTTTAAGTAATTTAGAAGATCATGAAAAAAACACAATTAAAGCAAATTCAATTGAAATGCGCCAATACAATTTAAAAAAGTTGGTGAAAATTAAAAATTTGACAAAAGAATTCAAAATAAAAAAAGAAAAAATAAAAGTTGTAGATAATTTGAATCTCGAAATTTATAAGAATCAAAACATTGCTCTATTAGGTGGAAATGGAGCCGGAAAAACTACAACAGTTGAAATGCTTATAGGAATTTTAAAACCTACCTCAGGCAATATTGAATATTATTTTAATGATGGATGA", "aa": "MENEKVCKNKSLEIIDNFLSNLEDHEKNTIKANSIEMRQYNLKKLVKIKNLTKEFKIKKEKIKVVDNLNLEIYKNQNIALLGGNGAGKTTTVEMLIGILKPTSGNIEYYFNDG", "aa_hexdigest": "73eb6e1d5a45b71fc1749c8dfc95c1ff", "start_type": "GTG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 625933, "stop": 626515, "observations": { "insertion": [ 625972 ], "mutation": [ 626176 ], "stop": [ 626176 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A5ABD3", "query_cov": 1.0, "subject_cov": 0.5885416666666666, "identity": 0.991, "score": 213.0, "evalue": 6.18e-69, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MENEKACKNKSLEIIDNFLSNLEDHEKNTIKANSIEMRQYNLKKLVKIKNLTKEFKIKKEKIKVVDNLNLEIYKNQNIALLGGNGAGKTTTVEMLIGILKPTSGNIEYYFNDGSNHNESAQTNIGIQFQDSSYPQGLSVNDVIYSMNKIYGNKSSPEELQKLIKIFGVDEFINNKASSLSGANIKDLMHCLQ" }, "paralog": false, "identity": 0.9844559585492227, "score": 368.0, "evalue": 2.01e-132, "description": "Internal stop codon at 626176. Frameshift due to insertion around 625972. Nonsense mutation around 626176.", "psc": { "uniref90_id": "UniRef90_UPI0034A5ABD3", "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI0034A5ABD3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A5ABD3", "UniRef:UniRef50_UPI0034A5ABD3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A5ABD3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A5ABD3" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "DFOKDJOJIG_1098", "locus": "GCA013367855_01097" }, { "type": "cds", "sequence": "contig_1", "start": 626622, "stop": 627224, "strand": "-", "frame": 2, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "db_xrefs": [ "COG:COG0344", "COG:I", "EC:2.3.1.15", "EC:2.3.1.275", "GO:0005886", "GO:0006654", "GO:0008374", "KEGG:K08591", "SO:0001217", "UniRef:UniRef50_D1J8M5", "UniRef:UniRef90_D1J8M5" ], "nt": "TTGGCAATAATAATAACTAAATTAAGAGGGAATGATATAAGAAAAATTGGCTCGCAAAATCCTGGAGCAACAAACGCATTAAGAAATTTTGGAATAAAATTTGCAGCATTAGTTTTTGCTTTTGACATTTTTAAAGCCTTTTTAGCAACAATAATTGCATTTTTAATTAAAAAATATCTAAGAAATCTTGAATATATTTTTCCAATGCTAGCAGGGCTTGGTGCAGTAATTGGACACGTCTTTCCAATTTATTTTAAATTTAAAGGCGGCAAAGGAGTAGCTTGCTTTTTTGGAATAATGCTTGCATATAATTTTCTATTATTTTTAATTTTTGTAGCAATATATGTAATACTAGTTTTTTCAACAAAATATGTTTCACTTGCTTCATCAATAAGCTGCGGAATAATTCCTTATTTAGGTTTAATTTATATATTTTATGCAGGAACAATATTTGGTTATATGCAACAAAATGCTATATATCCCGCACATGTAATTATTTTAATATTGGCTTCGCTAATAATAGTTGTTAAACATATACCCAATTTTAAACGAATATTTAAACATACAGAACCAAAAATTTTTAGCAAAACAAAAAAAGCATAA", "aa": "MAIIITKLRGNDIRKIGSQNPGATNALRNFGIKFAALVFAFDIFKAFLATIIAFLIKKYLRNLEYIFPMLAGLGAVIGHVFPIYFKFKGGKGVACFFGIMLAYNFLLFLIFVAIYVILVFSTKYVSLASSISCGIIPYLGLIYIFYAGTIFGYMQQNAIYPAHVIILILASLIIVVKHIPNFKRIFKHTEPKIFSKTKKA", "aa_hexdigest": "247252e5ff46395546bc9564bb3e0257", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8M5", "query_cov": 0.995, "subject_cov": 0.868995633187773, "identity": 0.985, "score": 368.0, "evalue": 6.31e-128, "valid": true, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "ec_ids": [ "2.3.1.15", "2.3.1.275" ], "uniref50_id": "UniRef50_D1J8M5", "cog_id": "COG0344", "cog_category": "I", "kegg_orthology_id": "K08591", "go_ids": [ "GO:0005886", "GO:0006654", "GO:0008374" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M5" ], "product": "Glycerol-3-phosphate acyltransferase" }, "genes": [ "plsY" ], "id": "DFOKDJOJIG_1099", "locus": "GCA013367855_01098" }, { "type": "cds", "sequence": "contig_1", "start": 627372, "stop": 627749, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_036438733.1", "SO:0001217", "UniParc:UPI000513208E", "UniRef:UniRef100_A0A454CA59", "UniRef:UniRef50_D1J8M6", "UniRef:UniRef90_D1J8M6" ], "nt": "ATGGAATTATTAAAATATAGTTTAATTACAAAAAACGAATTCAATAATGAACCCTCTAAAACGATAGAAACAGAATTTGTTCCATATACTGAAGAAGTTGTTGATGAATTTATTCATATAAATTTTATTGATAATAATAATCAGGAATGTGATTTATTGGTCAATAGTGATACTTTAAAAATAAAATATTCTGGTTCATATATCGAGTTACAAAAAAACAAAAGAGTAAGCAATAAAATTCAAGAATTTTCTCTAGATTTTTATTTAAAAGATGTTTTTATTGAAGGAAACAAGATATCTGCTCATTATGATATTTTAGTTGGTAGTGATGTTGTTGCAAGCAATATAATTAACATTGAAACAAAGACCGAAAAATAG", "aa": "MELLKYSLITKNEFNNEPSKTIETEFVPYTEEVVDEFIHINFIDNNNQECDLLVNSDTLKIKYSGSYIELQKNKRVSNKIQEFSLDFYLKDVFIEGNKISAHYDILVGSDVVASNIINIETKTEK", "aa_hexdigest": "df684aec66dd184348c9c9a0a8c296fb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000513208E", "ncbi_nrp_id": "WP_036438733.1", "uniref100_id": "UniRef100_A0A454CA59", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513208E", "RefSeq:WP_036438733.1", "UniRef:UniRef100_A0A454CA59" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CA59", "uniref90_id": "UniRef90_D1J8M6" }, "psc": { "uniref90_id": "UniRef90_D1J8M6", "uniref50_id": "UniRef50_D1J8M6" }, "pscc": { "uniref50_id": "UniRef50_D1J8M6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14520.160299999989, "isoelectric_point": 4.650191307067872 }, "id": "DFOKDJOJIG_1100", "locus": "GCA013367855_01099" }, { "type": "cds", "sequence": "contig_1", "start": 627730, "stop": 627846, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATTAGAAAGGCAACAACATTTGAGGAACATGGCCCTGAACTTGCAAAAGCAATCTATCTTTTTGGGGCTGAATTAGTTTTTAAGAGATTAAATGGAATTATTAAAATATAG", "aa": "MPIRKATTFEEHGPELAKAIYLFGAELVFKRLNGIIKI", "aa_hexdigest": "ee89e1d7e6ceb7981c380db406f08935", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4316.1174999999985, "isoelectric_point": 9.397636985778806 }, "id": "DFOKDJOJIG_1101", "locus": "GCA013367855_01100" }, { "type": "cds", "sequence": "contig_1", "start": 628270, "stop": 628386, "strand": "-", "frame": 1, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F", "UniRef:UniRef90_UPI003594285F" ], "nt": "ATGGAAGGAAAAAAGCTTTCAAAAAGAGATAAAACTTTAAAACAATTTATTGAAGACTATAAAAATGAAGGCTATCCCGCAGCAGCAATAATGAATTTTTTAGCCTTATTAGGTTGA", "aa": "MEGKKLSKRDKTLKQFIEDYKNEGYPAAAIMNFLALLG", "aa_hexdigest": "57ad0b88b87cbb936a0cd5b0b7ea7e03", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 628222, "stop": 628479, "observations": { "mutation": [ 628272, 628428 ], "stop": [ 628272, 628428 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI003594285F", "query_cov": 1.0, "subject_cov": 0.4470588235294118, "identity": 0.8420000000000001, "score": 63.2, "evalue": 3.54e-12, "gene_start": 1, "gene_end": 38, "reference_start": 32, "reference_end": 69, "reference_sequence": "EHITNTPKQLAIYDAFGWETPRFGHLTIITNMEGKKLSKRDKFLKQFIEDYKNEGYRPDAIFNFLVLLGWTSADKSEVMSHQEII" }, "paralog": false, "identity": 0.8, "score": 136.0, "evalue": 1.36e-43, "description": "Internal stop codon at 628272, 628428. Nonsense mutation around 628272, 628428.", "psc": { "uniref90_id": "UniRef90_UPI003594285F", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI003594285F", "UniRef:UniRef50_UPI003594285F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F" ], "product": "glutamate--tRNA ligase family protein" } }, "id": "DFOKDJOJIG_1102", "locus": "GCA013367855_01101" }, { "type": "cds", "sequence": "contig_1", "start": 628426, "stop": 628719, "strand": "-", "frame": 1, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001A2605D2" ], "nt": "TTGCAAATTTCTAATGAAGAAAAGCAAAGAAGAGAAGAAGCTAAAGAATATTCAATAAGATTAAAATTACCAGCAAATAAAGATTATGCTTGGAATGACTTAGTAAGAGGCAAAATCTCAGTTAATAGTGATGATATAGGAGATTTTGTTATTATGAAAAGTGATGGTTATCCAACATATAATTTTGCAGTTGTTGTTGATGACCACCAAATGGAAATTTCACATGTACTAAGAGGCGAAGAACACATCACAAACACGCCAAAGCAATTAGCAATATACGATGCATTTGGATGA", "aa": "MQISNEEKQRREEAKEYSIRLKLPANKDYAWNDLVRGKISVNSDDIGDFVIMKSDGYPTYNFAVVVDDHQMEISHVLRGEEHITNTPKQLAIYDAFG", "aa_hexdigest": "aff7873dc2ec53cc08c3191a1c34f16c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00056C184B", "query_cov": 0.8350515463917526, "subject_cov": 0.8181818181818182, "identity": 0.617, "score": 114.0, "evalue": 4.76e-31, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI001A2605D2" }, "pscc": { "uniref50_id": "UniRef50_UPI001A2605D2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001A2605D2" ], "product": "glutamate--tRNA ligase family protein" }, "genes": [], "id": "DFOKDJOJIG_1103", "locus": "GCA013367855_01102" }, { "type": "cds", "sequence": "contig_1", "start": 628936, "stop": 629127, "strand": "-", "frame": 1, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7", "UniRef:UniRef90_UPI002208A1AD" ], "nt": "ATGAAAACTATAAGAACAAGATATGCACCGAGCCCAACTGGGTATTTGCACATTGGTGGTGCAAGAACTGCATTATTTAACTATTTATTTGCAAAACATTTCAATGGCACTTTTGTTTTTAGGCTAGAAGATACTGATGTTGCTAGAAACGTTGTTGGAGGTGAAGAAAGCCAATTAAATAATTTAGCGTGA", "aa": "MKTIRTRYAPSPTGYLHIGGARTALFNYLFAKHFNGTFVFRLEDTDVARNVVGGEESQLNNLA", "aa_hexdigest": "025c80f5c6cbd3489fb6fa238db8d3c4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0016AA1FE4", "query_cov": 0.9841269841269841, "subject_cov": 0.8611111111111112, "identity": 0.613, "score": 81.6, "evalue": 3.58e-19, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A5J4P5N7" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 628762, "stop": 629118, "observations": { "mutation": [ 628938 ], "stop": [ 628938 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002208A1AD", "query_cov": 0.9523809523809523, "subject_cov": 0.4444444444444444, "identity": 0.9, "score": 114.0, "evalue": 2.38e-31, "gene_start": 4, "gene_end": 63, "reference_start": 3, "reference_end": 62, "reference_sequence": "MKIRTRYAPSPTGYLHIGGARTALFCYLYAKHFNGQFIFRLEDTDVKRNVPGGEESQLNNLAWLGIIPDESPLNPNPIYGKYRQSEKLDRYWKIANELIEKGFAYKAYDNSDELEQQKKKVMKKEYLVLGMINID" }, "paralog": false, "identity": 0.7711864406779662, "score": 185.0, "evalue": 9.27e-62, "description": "Internal stop codon at 628938. Nonsense mutation around 628938.", "psc": { "uniref90_id": "UniRef90_UPI002208A1AD", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002208A1AD", "UniRef:UniRef50_A0A654IDE7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7" ], "product": "Glutamate--tRNA ligase" } }, "id": "DFOKDJOJIG_1104", "locus": "GCA013367855_01103" }, { "type": "cds", "sequence": "contig_1", "start": 629450, "stop": 629992, "strand": "-", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M8", "UniRef:UniRef90_D1J8M8" ], "nt": "ATGAAAAAACAAGCAATTATGACAAAATTTTTACTTTGTATAGGAAGTGTTGCTCCTGTGTTTGCATTGCCACTAGTTGCGGCAAGCTGTAATAAAACAGCAACCATAACTCTCAATTCAAAAGCGAAAGAATCATTTGAAAAATATGGTTATCACGTTAAGGGTTCTGCACTTGATTTTAATAAAAATAATTATTCAGTAAGTAACCCGATATCGCCAAGAGATTCCTTGTACAAATTAACAAAAAATTTAAAAGATGAAAAAGGAAATCCAATTTATTTTAGAGATGCCAACGGAAATTTATTAAAATTTAAGGGTAAAAAATTGGAAAATGGTGAAATAGAATTTGATAGAGATGCCAACGGAAACTACATTTATGACAAGGAAAATGGATATCCAAGATTGAAAGAAGTATTGAATAATGAACTCGAAGTAATTGACGGAAAACCAGTGCAAAGAGTTTATAGAGAATTTTTTAGCAGATTTTTAGATTTTAATAATATTTCTCCAAATTACGATTTTAGAGTTTTCTCTTTTACATGA", "aa": "MKKQAIMTKFLLCIGSVAPVFALPLVAASCNKTATITLNSKAKESFEKYGYHVKGSALDFNKNNYSVSNPISPRDSLYKLTKNLKDEKGNPIYFRDANGNLLKFKGKKLENGEIEFDRDANGNYIYDKENGYPRLKEVLNNELEVIDGKPVQRVYREFFSRFLDFNNISPNYDFRVFSFT", "aa_hexdigest": "b734f13cf62418a6f3f6c36a473dbf86", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 629147, "stop": 629992, "observations": { "mutation": [ 629188, 629233, 629323, 629356, 629401, 629452 ], "stop": [ 629188, 629233, 629323, 629356, 629401, 629452 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M8", "query_cov": 1.0, "subject_cov": 0.6360424028268551, "identity": 0.9940000000000001, "score": 340.0, "evalue": 1.71e-116, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MKKQAIMTKFLLCIGSVAPMFALPLVAASCNKTATITLNSKAKESFEKYGYHVKGSALDFNKNNYSVSNPISPRDSLYKLTKNLKDEKGNPIYFRDANGNLLKFKGKKLENGEIEFDRDANGNYIYDKENGYPRLKEVLNNELEVIDGKPVQRVYREFFSRFLDFNNISPNYDFRVFSFTWEELINYFPATKNDSRYWPYRDNPRALFLVLYWTLKQNEAAPNWKSDFLDPSIESLLAKGKNRNVNTSIEEAPWPFYKGIMKGNKDAFWSNASEPITAIFEKP" }, "paralog": false, "identity": 0.9715302491103203, "score": 525.0, "evalue": 1.11e-191, "description": "Internal stop codon at 629188, 629233, 629323, 629356, 629401, 629452. Nonsense mutation around 629188, 629233, 629323, 629356, 629401, 629452.", "psc": { "uniref90_id": "UniRef90_D1J8M8", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J8M8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M8", "UniRef:UniRef50_D1J8M8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M8" ], "product": "Variable surface lipoprotein" } }, "id": "DFOKDJOJIG_1105", "locus": "GCA013367855_01104" }, { "type": "cds", "sequence": "contig_1", "start": 630159, "stop": 630662, "strand": "+", "frame": 3, "gene": null, "product": "cysteine peptidase family C39 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E465ED2", "UniRef:UniRef90_UPI001E465ED2" ], "nt": "ATGATTTGCTACTTTTTTAAATATAAGGCAGATATAAATTTTTTAAAGAAAAATACGATATACGACGAAGAAGGAGTGTCGCTATATTCATTTAAAAAAATTTCTGATTTTGTAGGGTTTGATACTAATATTTACAAAGTAGATTTTTTAAGTTTTATTTCAAATTTGAATGATATTTCATTTCCGTTTGTCACAATATTGAAAAATGATAATTTCTTGCATTATACAATTGTTAAAAGAATTGATAAAGCCTTTGTTTATCTATGTGATTCTGAATTTTTGAATTATAGATTAGAAATTAATGAATTTAAAAATAAATTCATAAATGTTGTAGTTATATTAAGTAAAAAAATTAAAACCCCAAATTGCACAAAAATTAACTATAATCTATATCTAACACAAAACACAAAGTTAACACTAATTAGTATATTTTTGGCAATTTTATTCAATGCTCTTATGCTTTCTTCTTCATTTTATATTAAATATGTTTTAAGCGTTTTATGA", "aa": "MICYFFKYKADINFLKKNTIYDEEGVSLYSFKKISDFVGFDTNIYKVDFLSFISNLNDISFPFVTILKNDNFLHYTIVKRIDKAFVYLCDSEFLNYRLEINEFKNKFINVVVILSKKIKTPNCTKINYNLYLTQNTKLTLISIFLAILFNALMLSSSFYIKYVLSVL", "aa_hexdigest": "9f05db55b3167d874db6dbf2bbd7c29f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 630102, "stop": 630942, "observations": { "insertion": [ 630924 ], "mutation": [ 630105, 630660, 630711, 630738 ], "stop": [ 630105, 630660, 630711, 630738 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E465ED2", "query_cov": 1.0, "subject_cov": 0.5985663082437276, "identity": 0.97, "score": 314.0, "evalue": 1.95e-106, "gene_start": 1, "gene_end": 167, "reference_start": 20, "reference_end": 186, "reference_sequence": "MWINKQDSVKDCAIYTLQAMICHFFKYKADINFLKKNTIYDEEGVSLYSFKKISDFVGFDTNIYKVDFLSFISNLNDVSFPFVTILKNDNFLHYTIVKRIDKAFVYLCDSEFLNYRLEINEFKDKFINVVVILSKKFKTPNCTKINYNLYLTQNTKLTIISIFLAILFNALMLSSSFYIKYVLSVLWSENANNRLLSILIIFSWIAILKAITWLFKKIIINKLTFYYEKKLYLLVKERLKFASQKDLQKINQNDFMARILSISTITEYKAGLFLFYLKI" }, "paralog": false, "identity": 0.956989247311828, "score": 513.0, "evalue": 3.05e-187, "description": "Internal stop codon at 630105, 630660, 630711, 630738. Frameshift due to insertion around 630924. Nonsense mutation around 630105, 630660, 630711, 630738.", "psc": { "uniref90_id": "UniRef90_UPI001E465ED2", "product": "cysteine peptidase family C39 domain-containing protein", "uniref50_id": "UniRef50_UPI001E465ED2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E465ED2", "UniRef:UniRef50_UPI001E465ED2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E465ED2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E465ED2" ], "product": "cysteine peptidase family C39 domain-containing protein" } }, "id": "DFOKDJOJIG_1106", "locus": "GCA013367855_01105" }, { "type": "cds", "sequence": "contig_1", "start": 630798, "stop": 631253, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTCAAAGAAAGATTAAAATTTGCAAGTCAGAAAGACCTGCAAAAAATTAACCAAAATGATTTTATGGCAAGAATATTAAGTATTCCAACCATTGCAGAATATAAGGCGGGTTTATTTTTGTTTTTATTTGAAAATATAATTATTTTTATTAGTTCAACACTGCTTTTAGGTTTGTTTAATTATAGAATATTATTAATTTGCTTATTAGGCATTTCAATAACCTTTGCAATCTCGTTATTCAGTTTAAAATATCAATCAAAAAAAGCAACAGAATTAATAAATTTAAAGATAAAATCAATTAAAATATTCAATAATTATTTTTTAAATTATGAATATAGGACGAACGATTTAAATAATTCGAATTCTAAATTATTTCAATCATTATTTAACACTCAATATAATCTAGGAATATTCAATGAAACAAAAGATTCATTTATTACATTTATTTGA", "aa": "MLVKERLKFASQKDLQKINQNDFMARILSIPTIAEYKAGLFLFLFENIIIFISSTLLLGLFNYRILLICLLGISITFAISLFSLKYQSKKATELINLKIKSIKIFNNYFLNYEYRTNDLNNSNSKLFQSLFNTQYNLGIFNETKDSFITFI", "aa_hexdigest": "378944cd501bea68bb8ed99a8718769b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17657.563199999982, "isoelectric_point": 9.546881294250486 }, "id": "DFOKDJOJIG_1107", "locus": "GCA013367855_01106" }, { "type": "cds", "sequence": "contig_1", "start": 631293, "stop": 631646, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E29CFE5", "UniRef:UniRef90_UPI001E29CFE5" ], "nt": "TTGTTTATAAAAAATAAAGATAATTTTACAAATATTACATTATATGGATCAATATCAATTTTTTTGAATTCCCCTATTGTAAATTTAGTCTTCCTGATTTCAAATTCTTCAATAAATAAACAGGGAATAATAAGAATAAAATATTTATTAGAAATCACACAAAACAAGGATGGAAATATATCATTCAAACCCCAGAAGCAAAATAAAATAAACATATTAAATGCTTCAATAAATATTGGTTCAAAAAACGTTATAAAAAATCTTAATTTAACAATCAAAAACAATCTAGAAATATCAGGAAAAAATGGAGTTGGTAAAAGTTTAATTTTGAATGCTATATTTAAAGTTTATTGA", "aa": "MFIKNKDNFTNITLYGSISIFLNSPIVNLVFLISNSSINKQGIIRIKYLLEITQNKDGNISFKPQKQNKINILNASINIGSKNVIKNLNLTIKNNLEISGKNGVGKSLILNAIFKVY", "aa_hexdigest": "d55d977f35d5f1a57f76ff395687abdc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 631107, "stop": 631926, "observations": { "insertion": [ 631911 ], "mutation": [ 631251, 631290, 631644, 631707 ], "stop": [ 631251, 631290, 631644, 631707 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E29CFE5", "query_cov": 1.0, "subject_cov": 0.43014705882352944, "identity": 0.983, "score": 215.0, "evalue": 2.27e-68, "gene_start": 1, "gene_end": 117, "reference_start": 63, "reference_end": 179, "reference_sequence": "MFNNYFLNYEYRTNDLNNSNSKLFQSLFNTQYNLGIFNETKDSFITFIWDFIQLTIIFTGAWLFIKNKDNFTNITLYGSISIFLNSPIVNLVFLISNSSINKQGIIRIKYLLEITQNKDGNISFKPRKQNKINILNASINIGSKNVIKNLNLTIKNNLEISGKNGVGKSLILNAIFKVYWLNQGHIFVNNINLKHISQKSWLDACFLNKNNEYFCYENLMDCICKKDNIKENIFLKNFKKYNLNNLFLDMNLPLNKYIENGGNNLSSGRRNS" }, "paralog": false, "identity": 0.9742647058823529, "score": 521.0, "evalue": 1.04e-191, "description": "Internal stop codon at 631251, 631290, 631644, 631707. Frameshift due to insertion around 631911. Nonsense mutation around 631251, 631290, 631644, 631707.", "psc": { "uniref90_id": "UniRef90_UPI001E29CFE5", "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI001E29CFE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E29CFE5", "UniRef:UniRef50_UPI001E29CFE5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E29CFE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E29CFE5" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "DFOKDJOJIG_1108", "locus": "GCA013367855_01107" }, { "type": "cds", "sequence": "contig_1", "start": 631764, "stop": 632120, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTGCATATGTAAGAAAGACAATATAAAGGAAAATATTTTTCTTAAAAATTTCAAAAAATATAATTTAAATAACTTGTTTTTAGATATGAACTTACCTCTTAATAAATATATTGAAAATGGAGGAAATAACCTATCATCCGGCCAGAAGGAATTCATAATTATAATGCGACTTTTTTGCCAGAAATATTATTTTATTCTTTTGGATGAGGCTTTTGAAAACATTACAAACGAAATTTTTCTTAAATTAAAATTTTTAATTTTAAACTACCAAGATAGGGCCATTTTTATTGAAATTAGCCATAACAAAAAAATTGTTGCAAACAAGCGAAAAATTTTCGCTTTAAAAAAATAA", "aa": "MDCICKKDNIKENIFLKNFKKYNLNNLFLDMNLPLNKYIENGGNNLSSGQKEFIIIMRLFCQKYYFILLDEAFENITNEIFLKLKFLILNYQDRAIFIEISHNKKIVANKRKIFALKK", "aa_hexdigest": "ad3492312072d939909b2d012609c73b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14151.632599999977, "isoelectric_point": 9.444441032409667 }, "id": "DFOKDJOJIG_1109", "locus": "GCA013367855_01108" }, { "type": "cds", "sequence": "contig_1", "start": 632179, "stop": 632631, "strand": "+", "frame": 1, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "GO:0004540", "GO:0042254", "GO:0046872", "RefSeq:WP_114091069.1", "SO:0001217", "UniParc:UPI000DED9669", "UniRef:UniRef100_UPI000DED9669", "UniRef:UniRef50_A0A448ZWQ4", "UniRef:UniRef90_D1J8N0" ], "nt": "ATGAATAGAATTAATTTTAAAAACCAAAGCTTTCATCGTTTTAATTTTATGAAAGATTTCTTAGATATTTTAGAAGAAGCAAAAATAGAATTTGGTGCAAAAAAATCACTTTGTGTTGATGTAATGTTTGTAACTGCTAAAAAGATGAAAAAGTTAAATAACTTACATAGACAAAAAAACTATACAACTGATATTTTAAGTTTCCCACTAGATAGTCAAGAAGAATTAAATTTTTTGGATGAAATTGAATTAGGACAAATTATTATTTCTCCGTGGAAAATAAAAAAACAAGCCAAGGAGTTCAATCATAGTTTAAGACGAGAATTTTGTTATATTTTTGCTCATGGTATTGCTCACCTATTTGGTTTTGATCACATTACTGAAGAAGAAGCAAAAATAATGAATGGGCATGTAGATAACATAATGAATAAATTAAACATAAAGAGAATATAA", "aa": "MNRINFKNQSFHRFNFMKDFLDILEEAKIEFGAKKSLCVDVMFVTAKKMKKLNNLHRQKNYTTDILSFPLDSQEELNFLDEIELGQIIISPWKIKKQAKEFNHSLRREFCYIFAHGIAHLFGFDHITEEEAKIMNGHVDNIMNKLNIKRI", "aa_hexdigest": "b88adbbd6677ee0efcad34367545dd7c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DED9669", "ncbi_nrp_id": "WP_114091069.1", "uniref100_id": "UniRef100_UPI000DED9669", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DED9669", "RefSeq:WP_114091069.1", "UniRef:UniRef100_UPI000DED9669" ] }, "ips": { "uniref100_id": "UniRef100_UPI000DED9669", "uniref90_id": "UniRef90_D1J8N0" }, "psc": { "uniref90_id": "UniRef90_D1J8N0", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "uniref50_id": "UniRef50_A0A448ZWQ4", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004540", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZWQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZWQ4" ], "product": "Endoribonuclease YbeY" }, "genes": [ "ybeY" ], "id": "DFOKDJOJIG_1110", "locus": "GCA013367855_01109" }, { "type": "cds", "sequence": "contig_1", "start": 632645, "stop": 633490, "strand": "+", "frame": 2, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "GO:0003723", "GO:0005525", "SO:0001217", "UniRef:UniRef50_A0A501XB37", "UniRef:UniRef90_D1J8N1" ], "nt": "ATGAAAAAAGTTTGCAATGTATGCTTAATTGGGCGCCCAAATGTTGGAAAAAGCACGTTTTTAAATAATGTGTTAAATTTTAACCTATCAATAATTACGGATAAACCCCAAACAACAAAAGATAACATTAGAGGAATATACAATGATAAAGACTATCAAATCATTTTTATTGATACTCCAGGAATTCACAAAGCAGAAAATTTATTATCTGAAAGATTAAATAGTAAATCATATGAAGCTATTGAAAATTCTGATGTTGTTTTGTTTTTAACTCCCGCTAATGAAACAATCGGAACAGGAGATAAATTCATTATTGAAAAAATAAACGAAACTAATAATGAAAATAAAATTGCAGTTATTTCAAAAATTGATTTATTAAATGTAAAAGAAGAAGCAGACAAAAAAGCAGAAGAATTAAAAAAACTTGGATTTGAAAAAGTTTTTGGCATTGGACAAAATTTGCCTACTTCTTATTTAGATTTGATTAATGAAATTAAAACCTATTGCTACGAAGATGAGCCATTGTACCCTGATGATCAAATTGGGGATATAACATTAAGGTTTATGGCAAAAGAAATAATTAGAGAAACTGCTATAAACAATTTAAAATTTGAATTACCACACTCAATTGCGGTTGAGATCGAACAATTTGATGATAAAAACGAAGATGAAGATTACAAGATCTATGCTTTAATTTATGTTAAAAAGAAGTCTCAGAAACCTATTTTAATTGGAAAAGACGGTCAAATGATTAAAAAAATCTCTATTGATTCACGACATAAAATGGAAAAAATTTTTAATCACAAAGTCTTTTTAAATATTAAAGTAAAAATTAGCGAAGATTGA", "aa": "MKKVCNVCLIGRPNVGKSTFLNNVLNFNLSIITDKPQTTKDNIRGIYNDKDYQIIFIDTPGIHKAENLLSERLNSKSYEAIENSDVVLFLTPANETIGTGDKFIIEKINETNNENKIAVISKIDLLNVKEEADKKAEELKKLGFEKVFGIGQNLPTSYLDLINEIKTYCYEDEPLYPDDQIGDITLRFMAKEIIRETAINNLKFELPHSIAVEIEQFDDKNEDEDYKIYALIYVKKKSQKPILIGKDGQMIKKISIDSRHKMEKIFNHKVFLNIKVKISED", "aa_hexdigest": "5dfcc596ed9661abbe513785921c3e51", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8N1", "query_cov": 1.0, "subject_cov": 0.95578231292517, "identity": 0.993, "score": 540.0, "evalue": 1.32e-193, "valid": true, "gene": "era", "product": "GTPase Era", "uniref50_id": "UniRef50_A0A501XB37", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501XB37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501XB37" ], "product": "GTPase Era" }, "genes": [ "era" ], "id": "DFOKDJOJIG_1111", "locus": "GCA013367855_01110" }, { "type": "cds", "sequence": "contig_1", "start": 633531, "stop": 634235, "strand": "+", "frame": 3, "gene": null, "product": "Hypothetical lipoprotein (MG045 family)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9E7F", "UniRef:UniRef90_UPI00215D9E7F" ], "nt": "GTGAAACGATTAACTACACATAGAACGAGAGTTATTTTTATAGCAATAATTGCCTTACTAACACTTGGATTATTTTTAATAATTTTATTTTTTAAAATTAGCTCCCCATTTAAAGTAAGTATATATAACTACGAATCATACTTAGATAAAAAAATAATTACTAAATTAAAACAAAATTATTCTTATCACGCCTTTACAAATCTAGATGAGTTTACGAGAGCAATAAATAACGAAAAAGCCGTTGCCGGAGTTGGAAGTGATTACCAAATTGCTCAACTAATTCTTGAAAAAAAATTAAGAAAAATTAACTTTGCAAAATTGTTTAATAATTCAAAATTTAATGATTCAACCCAAATAAAGAATCTTTATCCAGAAATTGTTAATAAACATTTTGATTTATTTGAAAAATTTATCATTAAAAAAATAAGACAAATAAATCCTGAGAACTTCCAGCAAACTGATTGGCAAAATATTAGTCACACCAAATTCTTACCTTTTATTTATTATGACAAAAATAACAATATTTTAGGTTTTGAAATTGATCAAAAAAAGGGAATTGACCACTTTTATGAATTTTTAATTCCTTATTTTATAATGGATAAAATGGTTGTTTATAATACAGATAAAACCCCAAATAAATTTACTGAAAGAAGCAATTTAAAATCTAATGAAAACTTTAACGATTTAGAAAGTTTAAATTCTTGA", "aa": "MKRLTTHRTRVIFIAIIALLTLGLFLIILFFKISSPFKVSIYNYESYLDKKIITKLKQNYSYHAFTNLDEFTRAINNEKAVAGVGSDYQIAQLILEKKLRKINFAKLFNNSKFNDSTQIKNLYPEIVNKHFDLFEKFIIKKIRQINPENFQQTDWQNISHTKFLPFIYYDKNNNILGFEIDQKKGIDHFYEFLIPYFIMDKMVVYNTDKTPNKFTERSNLKSNENFNDLESLNS", "aa_hexdigest": "66d27bd6abb86ac7beff2cede0ae5f1e", "start_type": "GTG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 633531, "stop": 634538, "observations": { "mutation": [ 634233, 634287, 634296 ], "stop": [ 634233, 634287, 634296 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D9E7F", "query_cov": 1.0, "subject_cov": 0.612565445026178, "identity": 0.983, "score": 449.0, "evalue": 5.8e-157, "gene_start": 1, "gene_end": 234, "reference_start": 1, "reference_end": 234, "reference_sequence": "MKRLTTHRTRVIFIAIIALLTLGLFLIILFFKISSPFKVSIYNYESYLDKKIITKLKQNYSYHAFTNLDEFTRAINNEKAVAGVGSDYQIAQLILEKKLRKINFAKLFNNSKFNDSTQIKNLYPEIVNKHFDLFEKFIIKKIRQINPENFQQTDWQNISHTKFLPFIYYDKNNNILGFEIDQKKGIDHFYEFLIPYFIMDKMVVYNTDKSPNKFTERSNLKNNENFNNLESLSSWKDIIKTLVSKYKNPRVYWTNWFQDNAMIGQFYGYESLNKLKYFSKQNEWADINHENFKEIINDWVSLVKDATGESIKNSKTNKLATDGQELVSSIIEPINGKADVSIMYNGDALDAYYGEDNFKKLGNIPHIGFKRPKILIWILMLE" }, "paralog": false, "identity": 0.9761194029850746, "score": 655.0, "evalue": 6.02e-241, "description": "Internal stop codon at 634233, 634287, 634296. Nonsense mutation around 634233, 634287, 634296.", "psc": { "uniref90_id": "UniRef90_UPI00215D9E7F", "product": "Hypothetical lipoprotein (MG045 family)", "uniref50_id": "UniRef50_UPI00215D9E7F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D9E7F", "UniRef:UniRef50_UPI00215D9E7F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9E7F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9E7F" ] } }, "id": "DFOKDJOJIG_1112", "locus": "GCA013367855_01111" }, { "type": "cds", "sequence": "contig_1", "start": 634314, "stop": 634676, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGCCAATTTTATGGTTATGAATCTCTTAACAAATTAAAATATTTTTCTAAGCAAAACGAATGGGCAGATATAAATCACGAAAATTTTAAAGAGATTATTAATGATTGGGTTTCTTTAGTTAAGGATGCAACAGGAGAAAGTATAAAAAATTCTAAAACAAATAAATTAGCTACTGATGGACAAGAGTTGGTTAGTTCAATAATAGAGCCGATTAATGGCAAGGCAGATGTTTCAATTATGTATAACGGAGATGCTTTAGATGCTTATTACGGGGAAGACAATTTTAAAAAATTAGGTAATATTCCACATATTGGTTTTAAAAGGCCAAAAAATACTTATATGAATATTGATGCTTGA", "aa": "MIGQFYGYESLNKLKYFSKQNEWADINHENFKEIINDWVSLVKDATGESIKNSKTNKLATDGQELVSSIIEPINGKADVSIMYNGDALDAYYGEDNFKKLGNIPHIGFKRPKNTYMNIDA", "aa_hexdigest": "1d593f8f505bbeeb243748845f34f4e8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13645.133699999984, "isoelectric_point": 5.332657051086425 }, "id": "DFOKDJOJIG_1113", "locus": "GCA013367855_01112" }, { "type": "cds", "sequence": "contig_1", "start": 634797, "stop": 635195, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAATCTAATATCAAAAGACAACAAATTAAAAAAAGATGAAATTGTTAATGAGCTATTTGCAGACAAAAACATGTCTATGCCCAAAAAAATTAAAGACATTAAAAAAGATTTTTTTGCAGATTATTTTGACTATTTCAAAGAAGCATTTGAAACAATGAATTTACCCATTGTAAACAATTTTCATTCAATAAATTACACACCAGGCTTTAAAAATGTAAAAAATTTTATTGAAAAATGGTACTTTTTGAATAAAAATCAGGAAGTTGATAAAACTGCTTTGGAGATATTCAATCCAGAATTTAATAAAAAAATCAATCACAGAATTTACCAACCACTTGATTTAAATTTAAAAACAAAAATAATAGATTATTACTTTCAAAAAACAAAATCATAA", "aa": "MLNLISKDNKLKKDEIVNELFADKNMSMPKKIKDIKKDFFADYFDYFKEAFETMNLPIVNNFHSINYTPGFKNVKNFIEKWYFLNKNQEVDKTALEIFNPEFNKKINHRIYQPLDLNLKTKIIDYYFQKTKS", "aa_hexdigest": "f1cb5902fb61522a5cd0418642398d41", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15995.309899999986, "isoelectric_point": 9.204876518249513 }, "id": "DFOKDJOJIG_1114", "locus": "GCA013367855_01113" }, { "type": "cds", "sequence": "contig_1", "start": 635224, "stop": 635421, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGAAAATAAAATTAAAACACCAAACAAAAAAGAAGAACCAATAATTCAATTAGTAGACGTGGTTAAAGAATTTGAAGATAAAACAGTTTTAGATAATGTTAACTTGTCAATAAATAAAGGTGAATTTATAACATTACTAGGTCCGTCAGGTTCAGGAAAAACAACCATATTGAGAATTATTGGCGGTTTTGAATGA", "aa": "MENKIKTPNKKEEPIIQLVDVVKEFEDKTVLDNVNLSINKGEFITLLGPSGSGKTTILRIIGGFE", "aa_hexdigest": "1c35952b86a6c04b9beefa48b03e8b0b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 34, "aa_cov": 0.5230769230769231, "hmm_cov": 1.0, "evalue": 7.497698239202706e-11, "score": 38.44517135620117, "start": 31, "stop": 64 } ], "seq_stats": { "molecular_weight": 7200.265399999998, "isoelectric_point": 5.396600914001465 }, "id": "DFOKDJOJIG_1115", "locus": "GCA013367855_01114" }, { "type": "cds", "sequence": "contig_1", "start": 635854, "stop": 636213, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "nt": "ATGAACCAAGAAGTATCGGAAATCATGGATCTTGTTGGTTTGAAGGGAAATGAAAAGAAAAATGTTAATTTCCTATCTGGTGGTATGAAACAACGTGTTGCACTTGCTCGCTCTCTTGTTATTGAACCCGAAATTCTTCTACTAGATGAACCATTAAGTGCCTTAGATGCTAAAATTAGACAAAAAATGCAAACATTATTAAGGGATATTCAAAAAGAATTAAAAATTACGTTTATTTTTGTTACTCACGATAGAAATGAAGCATTGCAATTAAGTGATAGAATCGCCATTATCAGAGATGGTAAGGTTGAACAATTTGCTTCACCAAAAGATTTATACGATTATCCAATAAACAAATGA", "aa": "MNQEVSEIMDLVGLKGNEKKNVNFLSGGMKQRVALARSLVIEPEILLLDEPLSALDAKIRQKMQTLLRDIQKELKITFIFVTHDRNEALQLSDRIAIIRDGKVEQFASPKDLYDYPINK", "aa_hexdigest": "9efcb0bd6e594589d883b5805109b473", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00237D6FE8", "query_cov": 0.9747899159663865, "subject_cov": 0.9133858267716536, "identity": 0.517, "score": 122.0, "evalue": 8.09e-34, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00237D6FE8" }, "pscc": { "uniref50_id": "UniRef50_UPI00237D6FE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "DFOKDJOJIG_1116", "locus": "GCA013367855_01115" }, { "type": "cds", "sequence": "contig_1", "start": 636283, "stop": 636525, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAAAATTTTTGATACTATTCACAAAGAATTTGAGCCAGGAGAAAACCTTGATGCATTAATTAGACCCGAAGATGTTTATGTTACCAAAGTAAAAGCCAAATCTAAATTTATCGGCAAAATAATTAAATCAACATATAGTGGTAGTTACTACAATTATGAGGTTTTAGTAAAAAATAAAATAATAAAAATGGAAACATCAATAAACTATGAAGTGGGTAAGGAAGTATATTTAGATTGA", "aa": "MGKIFDTIHKEFEPGENLDALIRPEDVYVTKVKAKSKFIGKIIKSTYSGSYYNYEVLVKNKIIKMETSINYEVGKEVYLD", "aa_hexdigest": "a4241d06e7d04c26a50daf9835bcc07c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9271.616300000002, "isoelectric_point": 7.863031578063965 }, "id": "DFOKDJOJIG_1117", "locus": "GCA013367855_01116" }, { "type": "cds", "sequence": "contig_1", "start": 636602, "stop": 636847, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAACAACTGAAATCAACTTCTTATAAAGAAATAGACAAAAAAACTAAAAAAAGTAGTAAATTTGCAAAATTCTTTTTGGGTGCAAACAAATTACAACTTTCTTTGCTGATTCCTTATTTAGTATTTTCAATTTTATTTATTATTTTGCCATTAATTTTGATATTTATTTATGCATTCAAACCACTAGAAGGACAAGGTCATCTTGCAAACTGGGAAATAGCAGGCAAATCATCAACCTGA", "aa": "MEKQLKSTSYKEIDKKTKKSSKFAKFFLGANKLQLSLLIPYLVFSILFIILPLILIFIYAFKPLEGQGHLANWEIAGKSST", "aa_hexdigest": "956c3b2a5d780b4c58b591af23807aa1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9237.969099999991, "isoelectric_point": 9.824095344543458 }, "id": "DFOKDJOJIG_1118", "locus": "GCA013367855_01117" }, { "type": "cds", "sequence": "contig_1", "start": 636848, "stop": 637501, "strand": "+", "frame": 2, "gene": "potB", "product": "Spermidine/putrescine ABC transporter permeaseprotein potB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8N4" ], "nt": "ATGATTATTCTTAGATCAATTGGAACCGGAATATGTGCAGCGTTTTTTGCATTAATCATTGGTTTTCCATATGCCTATATTATTGCAAGAAGTAAATCAATGATATTTAGAACTTTAGGATTAACATTGATTCTAAGCCCGCTTGCAATATTCACAATTTCAAAAGCTTTAGCAGTTAGAGGTTTATTTTCGGCTATATTTGACGAAAATGAATTAAATAATAATTTCTTTCTAATCTTTGGAATGGTGTATTTGTATATCCCTTTTATGGTAATGCCATTATATCAAGTTATTAAGGATATGCCTAACAATATAATTGAAGCATCTCAAGATTTGGGATATTCAAAAACACAAACTGTTTTTAAAGTTATATTGCCTTACTGCATGAAAGCTATATTAAGCGGTTTTGGAGTTGTTCTTATGATGTCAGCAACATCAATAATTCTTTCAGATAAATTGTTGCCAAATGGTAGTCAAAAGCAGCTTATAGGAAATCTTATAAATCAGTTTGCTAATACAGCAAACCCTTACGACTTAGCAAATGCCTCTACATTAGTTATTATCACTTTGGCTGTTTTATTAACAATATATGGAATTATTTATGGTATACCTTATTTAATTTCAAAAAGAAAGCAAGGAGGAATATATGAATAA", "aa": "MIILRSIGTGICAAFFALIIGFPYAYIIARSKSMIFRTLGLTLILSPLAIFTISKALAVRGLFSAIFDENELNNNFFLIFGMVYLYIPFMVMPLYQVIKDMPNNIIEASQDLGYSKTQTVFKVILPYCMKAILSGFGVVLMMSATSIILSDKLLPNGSQKQLIGNLINQFANTANPYDLANASTLVIITLAVLLTIYGIIYGIPYLISKRKQGGIYE", "aa_hexdigest": "e85be2a79d3e66b5ba532116f4c918c5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00352DB7BA", "query_cov": 1.0, "subject_cov": 0.8037037037037037, "identity": 0.843, "score": 357.0, "evalue": 1.38e-122, "valid": false, "product": "ABC transporter permease", "uniref50_id": "UniRef50_D1J8N4" }, "pscc": { "uniref50_id": "UniRef50_D1J8N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8N4" ], "product": "Spermidine/putrescine ABC transporter permeaseprotein potB" }, "genes": [ "potB" ], "id": "DFOKDJOJIG_1119", "locus": "GCA013367855_01118" }, { "type": "cds", "sequence": "contig_1", "start": 637494, "stop": 637631, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTAAAAAATTTTTGAAGTATTCTTATGTATTAATAATTCTTGCTTGTTTATATATTCCAATCATTTTTGGAACAATATATAGCTTCAACGCACCTTCTGACAAGGGAATATTTTCTGTAACCTCATGA", "aa": "MNKFKKFLKYSYVLIILACLYIPIIFGTIYSFNAPSDKGIFSVTS", "aa_hexdigest": "40b3e6b142f85f76aa021437701af697", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5156.148800000001, "isoelectric_point": 9.519095420837402 }, "id": "DFOKDJOJIG_1120", "locus": "GCA013367855_01119" }, { "type": "cds", "sequence": "contig_1", "start": 637643, "stop": 637891, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTAAAAAATATAAGATTTAAAACAATTGCTAACGTTAAACCAGTAATAACATCTGGATTAATTAAAGGAATATTTGATGTTGATTGTACATATGCTCTAACTGTTTTGTTTTTATGCCTTCAAAGTGCAAAAACCGTCATCAATGAAAGCAATATAACAAAAATAGATGTTGAAATTCCTAGTAAAAAAGAATTTACAAATGCAAGTCAATGTGATTTTGAAAAAAGTTCCACATATGCTTCATAA", "aa": "MVKNIRFKTIANVKPVITSGLIKGIFDVDCTYALTVLFLCLQSAKTVINESNITKIDVEIPSKKEFTNASQCDFEKSSTYAS", "aa_hexdigest": "0a377a976156663ff8933718199f26bc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9081.464299999994, "isoelectric_point": 8.486892127990725 }, "id": "DFOKDJOJIG_1121", "locus": "GCA013367855_01120" }, { "type": "cds", "sequence": "contig_1", "start": 637944, "stop": 638051, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCTTCCTTATGGAATATTAATTATGTTGCCAAAAAGTGATAAATTTTCAAAAAATATCTTTGAAGCTAGCCAAGATCTAGGTTATTCAAAATTTAAAACCTGA", "aa": "MTLPYGILIMLPKSDKFSKNIFEASQDLGYSKFKT", "aa_hexdigest": "b5c22e152d8ea6852ff6c3ac1d418191", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4012.6883, "isoelectric_point": 9.224861717224119 }, "id": "DFOKDJOJIG_1122", "locus": "GCA013367855_01121" }, { "type": "cds", "sequence": "contig_1", "start": 638073, "stop": 638204, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTTCAATAGGATTTACATTTGTAATCACAATGACACTTTCTTTTGATGATTTCATTATTACTAGAATTGTTTCAAATACAGAAACTTTGGGTACTCAATTATATGAAGGCCAATTTCAAGCATGA", "aa": "MLGSIGFTFVITMTLSFDDFIITRIVSNTETLGTQLYEGQFQA", "aa_hexdigest": "2f3fb28131a84edda84bf5710dc59e95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4807.450900000001, "isoelectric_point": 4.0500284194946286 }, "id": "DFOKDJOJIG_1123", "locus": "GCA013367855_01122" }, { "type": "cds", "sequence": "contig_1", "start": 638355, "stop": 638690, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGATCACGAATTTTAAAAGCTATTAGTTTAATATTTTTACCCTTGATTTTTATTGCATTATTCCTTGCTTTTTTAGTTATAAAATCAACTAATCAATATAGACCTAGCATCTATAACTATGAATCTTATTTAGCTCCAACCATTATTAATAAATTAAAATCAAAATACAATTTCAAAGAATTTAAAGAAATTAGTGAGTTTACTCAAGCATTATTACAAGATAAGGCTATTGCTGGAATTGGAAGCGACTTTCAAGCTGCTCAACTTATTTTAGATAGGAAGATTCAAAAAATAGATTTTAGTCTAGTTTATGGTGAAAAATACAAAGATTGA", "aa": "MRSRILKAISLIFLPLIFIALFLAFLVIKSTNQYRPSIYNYESYLAPTIINKLKSKYNFKEFKEISEFTQALLQDKAIAGIGSDFQAAQLILDRKIQKIDFSLVYGEKYKD", "aa_hexdigest": "5711953ca1b4a6999f26c7eacde63b4b", "start_type": "TTG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12894.031299999988, "isoelectric_point": 9.551523017883298 }, "id": "DFOKDJOJIG_1124", "locus": "GCA013367855_01123" }, { "type": "cds", "sequence": "contig_1", "start": 639339, "stop": 639455, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACAACGGTGATGCATTGGATGCATTCTATTCTGAAGATAATTTTCAATCGGTTGAAAGTGGTTTAATAAGGTTTATTAGACCTAAAAACACATATATGCTAATGGATAATTGA", "aa": "MYNGDALDAFYSEDNFQSVESGLIRFIRPKNTYMLMDN", "aa_hexdigest": "20c0efe0d8ec23b32898ec4eb584397d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.9336, "isoelectric_point": 4.232879447937012 }, "id": "DFOKDJOJIG_1125", "locus": "GCA013367855_01124" }, { "type": "cds", "sequence": "contig_1", "start": 639594, "stop": 639746, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAACAATTAAAACAATCTGCTGAAGCCTCATTTTTTGAAACTATACATAATATTGATAAAACCTTAAAAAATAAGTTAATTCAAGAATTTTTAAAAAATAAAGAATTTTTCAATATAAACGAAAAATCAACTAGTTTTGAAGAATGA", "aa": "MTKQLKQSAEASFFETIHNIDKTLKNKLIQEFLKNKEFFNINEKSTSFEE", "aa_hexdigest": "c2205a1656575510b7deb76e03c503f0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5968.699199999997, "isoelectric_point": 6.571235466003418 }, "id": "DFOKDJOJIG_1126", "locus": "GCA013367855_01125" }, { "type": "cds", "sequence": "contig_1", "start": 639867, "stop": 639971, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGATGCTTTTGCAAATAGTGAAATAGGAAGCATCTCTAATTTTGATTATGTGTCTTATACTCCGACCGAAAAAATAATTTATGACTTTATTAAAAAATGA", "aa": "MSDAFANSEIGSISNFDYVSYTPTEKIIYDFIKK", "aa_hexdigest": "6a71f98844c169c361765855b19144da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3895.3002000000015, "isoelectric_point": 4.436874580383301 }, "id": "DFOKDJOJIG_1127", "locus": "GCA013367855_01126" }, { "type": "cds", "sequence": "contig_1", "start": 640354, "stop": 640899, "strand": "+", "frame": 1, "gene": "asnS", "product": "asparagine--tRNA ligase", "db_xrefs": [ "COG:COG0017", "COG:J", "EC:6.1.1.22", "GO:0004816", "GO:0005524", "GO:0006421", "KEGG:K01893", "SO:0001217", "UniRef:UniRef50_Q4A9S1", "UniRef:UniRef90_D1J8N7" ], "nt": "ATGCAATTAGTTGTTAAGGGGGATCTAGCATCAAAGAAAGAAATCTCTGAAATTGCCATGGGTTCAGCAGTTAGTGTAATAGGTAAATTACACTTAACGCCAGAAGCAATGCAACCATTTGAACTAAGTGTCTCAAAATTTTTATACATAAACAATCCACAAGAAGATTTTCCAATCCAAAAGAAAGAAACATCTCTTGATTTTTTAAGAGAAATTCCCCAATTAAGACATAGAACAAGTTTATTTAGAGCAATTATGTTAATCAGAAACTCTCTTATGTTTGAAATTCATAAATATTTCCAATCTAAAGGCTTTTTGAATCTTTCATCTCCAATAATCACATCAAACGATGGTGAAGGAGCAGGAGAAACTCTACTTGTTGACGATGAAAGCAAAGATTATTTTTTCAAACAAAAAGCATTTCTAGGGGTAACCGGACAATTGCACGCCGAATCATATGCTGAAGGATTTAAAAAAGTTTATACATTCGGGCCAACATTTAGAGCCGAAAATTCTCACACTTCGCGTCATCTAGCAGAATTTTGA", "aa": "MQLVVKGDLASKKEISEIAMGSAVSVIGKLHLTPEAMQPFELSVSKFLYINNPQEDFPIQKKETSLDFLREIPQLRHRTSLFRAIMLIRNSLMFEIHKYFQSKGFLNLSSPIITSNDGEGAGETLLVDDESKDYFFKQKAFLGVTGQLHAESYAEGFKKVYTFGPTFRAENSHTSRHLAEF", "aa_hexdigest": "758f3f7ea52be6bf45cc6c15456d6cb4", "start_type": "ATG", "rbs_motif": null, "genes": [ "asnS" ], "truncated": "both", "pseudogene": { "start": 640213, "stop": 641202, "observations": { "mutation": [ 640282, 640897 ], "stop": [ 640282, 640897 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8N7", "query_cov": 1.0, "subject_cov": 0.40401785714285715, "identity": 0.9940000000000001, "score": 355.0, "evalue": 4.98e-120, "gene_start": 1, "gene_end": 181, "reference_start": 48, "reference_end": 228, "reference_sequence": "MNIKQLYKQISQLQENVELTIEGWIISNRGNDKIRFITINDGSTIANMQLVVKGELASKKEISEIAMGSAVSVIGKLHLTPEAMQPFELSVSKFLYINNPQEDFPIQKKETSLDFLREIPQLRHRTSLFRAIMLIRNSLMFEIHKYFQSKGFLNLSSPIITSNDGEGAGETLLVDDESKDYFFKQKAFLGVTGQLHAESYAEGFKKVYTFGPTFRAENSHTSRHLAEFWMMEPEVAFCDLKGIIEITDDMLKNVIANTLKNYPEEMKFLDSLQNNKLIESLNNFINKKLTIIEYKDVVENLKNYVDKFEEKDIFFGMDLASEHEKFLAENIVKGPVAVINYPKDIKAFYMYQNDDGKTVAAFDLLVPGIGELVGGSQRENRYQKLVDRINELKITQETLQWYLDLRRFGYAPSSGFGLGFERLVMYVTGTSNIRDVIPFPRVAGSIKM" }, "paralog": false, "identity": 0.9878419452887538, "score": 637.0, "evalue": 1.22e-233, "description": "Internal stop codon at 640282, 640897. Nonsense mutation around 640282, 640897.", "psc": { "uniref90_id": "UniRef90_D1J8N7", "gene": "asnS", "product": "asparagine--tRNA ligase", "ec_ids": [ "6.1.1.22" ], "uniref50_id": "UniRef50_Q4A9S1", "cog_id": "COG0017", "cog_category": "J", "kegg_orthology_id": "K01893", "go_ids": [ "GO:0004816", "GO:0005524", "GO:0006421" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8N7", "EC:6.1.1.22", "UniRef:UniRef50_Q4A9S1", "COG:COG0017", "COG:J", "KEGG:K01893", "GO:0004816", "GO:0005524", "GO:0006421" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A9S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A9S1" ], "product": "Asparagine--tRNA ligase" } }, "id": "DFOKDJOJIG_1128", "locus": "GCA013367855_01127" }, { "type": "cds", "sequence": "contig_1", "start": 640900, "stop": 641415, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATGGAACCAGAAGTGGCCTTTTGTGATTTAAAAGGTATCATTGAAATTACAGACGATATGTTAAAGAATGTTATTGCAAATACATTAAAAAACTATCCCGAAGAAATGAAATATTTGGATTCATTACAAAACAATAAATTAATCGAATCATTAAACAACTTTATCAATAAGAAATTAACAATAATTGAATATAAAGATGTTGTTGAAAATCTTAAGAACTATGTAGATAAATTTGAAGAAAAAGATATCTTTTTCGGAATGGACTTAGCTTCAGAACACGAAAAATTCCTTGCCGAAAACATCATAAAAGGCCCTGTTGCAGTTATTAATTATCCAAAAGACATAAAGGCTTTTTATATGTATCAAAATGATGATGGCAAAACAGTCGCTGCCTTTGACTTATTAGTACCAGGAATTGGCGAATTAGTAGGGGGAAGTCAACGTGAAAATAGATATCAAAAGCTTGTTGACAGAATCAATGAACTAAAAATCACTCAAGAAACATTACAATGA", "aa": "MMEPEVAFCDLKGIIEITDDMLKNVIANTLKNYPEEMKYLDSLQNNKLIESLNNFINKKLTIIEYKDVVENLKNYVDKFEEKDIFFGMDLASEHEKFLAENIIKGPVAVINYPKDIKAFYMYQNDDGKTVAAFDLLVPGIGELVGGSQRENRYQKLVDRINELKITQETLQ", "aa_hexdigest": "f3df620e0280380f9006749c60767d11", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 177, "aa_cov": 0.9824561403508771, "hmm_cov": 0.807909604519774, "evalue": 3.516364730957195e-21, "score": 71.16402435302734, "start": 1, "stop": 168 } ], "seq_stats": { "molecular_weight": 19765.480399999993, "isoelectric_point": 4.737552833557129 }, "id": "DFOKDJOJIG_1129", "locus": "GCA013367855_01128" }, { "type": "cds", "sequence": "contig_1", "start": 641566, "stop": 641955, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAATAATTTAACAATATTAATTCCTATATATCATCCAACTCTAACAATTAATGAGATATTAAAAAATTTGTATAAACAAAAACAACAAAATTTTAATTTAGTAATCGCTATAGATAAGCCTTCTGAATCAGAACTTTATGAATTAGAAAAAGCCAAAACCTTTTTCTCAAATAGACTTCAAATAATAATCAATACAAATCACCAACACATTGATTCAATAATTAGTAGTTGCTTAAACATTACAAAAACAAAATACACATTTATAATGTATTCATATATTGAAATTGAGTATGATTTTATTTCAAAAATAAATAAACTTTTATTAGAATTTAAAGATGTTGATATTATAAGTTTTGACGCATATACAAAGGGTATATCTTGA", "aa": "MQENNLTILIPIYHPTLTINEILKNLYKQKQQNFNLVIAIDKPSESELYELEKAKTFFSNRLQIIINTNHQHIDSIISSCLNITKTKYTFIMYSYIEIEYDFISKINKLLLEFKDVDIISFDAYTKGIS", "aa_hexdigest": "832ac88b1f4755a0e7b3b13d06f85896", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15202.394499999984, "isoelectric_point": 5.884904670715332 }, "id": "DFOKDJOJIG_1130", "locus": "GCA013367855_01129" }, { "type": "cds", "sequence": "contig_1", "start": 641956, "stop": 642198, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTCAACAAATATCCTAAACAAAATAAAGAAATAAATATTCATATTGAAGAAGAAAATGTTGCTAAAATAATTCCTTTTATGTACAATTTTATTGCAAAAACTTCTTTACTTATTGGTGCAACAAATCAACACAATAATAAACACCTTAGCGAGCAATATTCACCCAATATACTTTTTAAGACTTTAATTAATGCAAACAAAATTCTTTTAACAAAAGATATTCAAGTAATTGATTGA", "aa": "MNFNKYPKQNKEINIHIEEENVAKIIPFMYNFIAKTSLLIGATNQHNNKHLSEQYSPNILFKTLINANKILLTKDIQVID", "aa_hexdigest": "30606754ed0a59badd8a4bb2cce4762a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9327.695899999992, "isoelectric_point": 8.965183067321778 }, "id": "DFOKDJOJIG_1131", "locus": "GCA013367855_01130" }, { "type": "cds", "sequence": "contig_1", "start": 642271, "stop": 642456, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGAATTTACAACTCCTTTTTCAGAAAGTTATTATTATCTTGCAAAATTTTTAAATATAGCATATTGTTTCGCAGGTTTTTTAGGCCAATGTCAATTTAAATCAAACACAAAAAACTATCTAACCTTAAAAAACCTTAAAAAACATTTAGAATCATCGATAAGTGAAAACATTGAAGAATGA", "aa": "MHEFTTPFSESYYYLAKFLNIAYCFAGFLGQCQFKSNTKNYLTLKNLKKHLESSISENIEE", "aa_hexdigest": "67d0416c72beeeb33e93d9cd4cbd7b03", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7182.0805999999975, "isoelectric_point": 6.712196159362793 }, "id": "DFOKDJOJIG_1132", "locus": "GCA013367855_01131" }, { "type": "cds", "sequence": "contig_1", "start": 642894, "stop": 643121, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATTTTTGCTTTATTTATGATTTTTATATCTCCCCAAACTTTTGAACAAGCAGCAAATCTTTCTCAAACAAAACCTCTAGACCAACTTCAAAAGGCTTTTCTTTCAATTCCTAGTGTGCTTTCGGCATTAGCCACATTTTTAGAATTCTTATTTATTTTAAGCAATGCTAAGGGAGATAGAATTGGTTTAAATGATAAATTTTCCGGATCCTATACAGTTTGA", "aa": "MIIFALFMIFISPQTFEQAANLSQTKPLDQLQKAFLSIPSVLSALATFLEFLFILSNAKGDRIGLNDKFSGSYTV", "aa_hexdigest": "3db174ad3cb0cf7179a09b67ef4a6051", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8319.641199999993, "isoelectric_point": 6.000572013854979 }, "id": "DFOKDJOJIG_1133", "locus": "GCA013367855_01132" }, { "type": "cds", "sequence": "contig_1", "start": 643346, "stop": 644026, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF00580.27" ], "nt": "ATGATTGATTTAAAAGATTTAAATGAACAGCAAAAAAGCGCCGTTATATATAACGAAGGACCTTTAAGAATTATTGCTGGCGCTGGTTCAGGAAAAACTAGAGTTTTAACATATAAGATTGCTTATTTAATCCAAAAATTAGGTGTTCTACCTAATCGTATTTTGGCACTAACTTTTTCAAATAAGGCTGCAAACGAAATGAAACAAAGGGTATTTAATCTACTTGCAGAAGATAACCCTAGCAATCTTTCTCCTTATATTTCAACATTCCATGCAATGTGTGCAAAAATATTGAGAAAAGAAATATATAACTTTGGATATGAAAACGATTTTCAAATTCTTGATGAATTAGACCAAAAAGAAATATTAAAAATTGTTTATTCAGAACTTGATATTTCGCCAACAGAATTTACATTCTCTTCAATAATTTCATTTATTCAAAACAAAAAGAATTCAATCGAATATTTAAACAACTCAATTAACGATAATAACGAACATTTTAATGAAGACTCAGATTCACAAGAAACACAAGATGAAAAAATCAAGGCAATAAAAGAAAAAATTTACGAACGCTACCAATTACACCTTGAAAGATCTCACTCCCTTGATTTTGATGATTTGTTGGTGTTTGTATACAAATTATTTTATGAACCAAAATTCAGTTTTGTAGCCGAAAGATGA", "aa": "MIDLKDLNEQQKSAVIYNEGPLRIIAGAGSGKTRVLTYKIAYLIQKLGVLPNRILALTFSNKAANEMKQRVFNLLAEDNPSNLSPYISTFHAMCAKILRKEIYNFGYENDFQILDELDQKEILKIVYSELDISPTEFTFSSIISFIQNKKNSIEYLNNSINDNNEHFNEDSDSQETQDEKIKAIKEKIYERYQLHLERSHSLDFDDLLVFVYKLFYEPKFSFVAER", "aa_hexdigest": "c8c0bd3435086c36cf028080ebc86a62", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 199, "aa_cov": 0.827433628318584, "hmm_cov": 0.8944723618090452, "evalue": 1.9347265719253246e-08, "score": 29.98868179321289, "start": 7, "stop": 193 }, { "id": "PF13245.12", "name": "AAA_19", "length": 123, "aa_cov": 0.5309734513274337, "hmm_cov": 0.5853658536585366, "evalue": 4.294387200998567e-14, "score": 48.503517150878906, "start": 11, "stop": 130 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 218, "aa_cov": 0.9336283185840708, "hmm_cov": 0.9220183486238532, "evalue": 3.1920543629790844e-50, "score": 166.92657470703125, "start": 7, "stop": 217 } ], "seq_stats": { "molecular_weight": 26346.566800000026, "isoelectric_point": 5.243078804016112 }, "id": "DFOKDJOJIG_1134", "locus": "GCA013367855_01133" }, { "type": "cds", "sequence": "contig_1", "start": 644183, "stop": 644386, "strand": "+", "frame": 2, "gene": null, "product": "3'-5' exonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00273A74DA" ], "nt": "ATGAATTTTGATAAGGATTACCCAAATTCAGTTACAGTAAAACTTGAAGAAAATTACAGATCAACCAAAACTATCTTAAGAGCAGCAAACAACTTAATTTCGCATAATAAACTTAGATTAGAAAAGAAATTATTCACTGAAAACGAAGAAGGCGAAGATATTGAGTTTTATTGTGGTTTTAATGACGAAGCAGAAGCTAGATGA", "aa": "MNFDKDYPNSVTVKLEENYRSTKTILRAANNLISHNKLRLEKKLFTENEEGEDIEFYCGFNDEAEAR", "aa_hexdigest": "97549997a635dfe7cb9efc2ab9e63df3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00273A74DA", "query_cov": 1.0, "subject_cov": 0.8701298701298701, "identity": 0.5670000000000001, "score": 73.9, "evalue": 5.36e-16, "valid": false, "product": "3'-5' exonuclease", "uniref50_id": "UniRef50_UPI00273A74DA" }, "pscc": { "uniref50_id": "UniRef50_UPI00273A74DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00273A74DA" ], "product": "3'-5' exonuclease" }, "genes": [], "id": "DFOKDJOJIG_1135", "locus": "GCA013367855_01134" }, { "type": "cds", "sequence": "contig_1", "start": 644993, "stop": 645583, "strand": "+", "frame": 2, "gene": null, "product": "DNA 3'-5' helicase II", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F3QEJ6" ], "nt": "TTGCAAGAAATAACTCTAATAACAGATAGAGATGTTGAAGATGATGCCGCAAGTTTCGTTTCACTAATGACTGTTCACAACTCAAAAGGGCTTGAATTTGATTATGTATTTGTTGCTTGCTTAGCAGAAAATATCTTTCCATTAAAGAGGGCCATATCGATTTCTCCACAAGACGATTTTACTTTTTTAAATTCAAAAAATTTCAAAGAAAACATAGAAGGATTAGAGGAAGAAAGACGTTTGGCTTATGTAGCAATGACTCGTGCTAAAAAAAGATTAATGCTTTCTTTCTCGGTTGGTAGAAATGGTATAAATAGAAAAAGTAGATTCTTGTCTGAAGCAGGAATAAACGAAATTAGAACAATAAAGATTGCCAACAACTTCTCAATTGCAGCAGAGCAAGAAACAAGAAATAACGATCTTATAGTTGGCGATTATATTATTCACAACACATATGGAAAAGGGTTGATTATAGATATGGTTGACAACGTTATTGAAGTTAAATTTGAAAATGATAAAAAGATTCGCAAATTATCAAAAACCCACCATTCAATTAAAAAATACGAAGATGATGAAAGCCTAAATAGTTAA", "aa": "MQEITLITDRDVEDDAASFVSLMTVHNSKGLEFDYVFVACLAENIFPLKRAISISPQDDFTFLNSKNFKENIEGLEEERRLAYVAMTRAKKRLMLSFSVGRNGINRKSRFLSEAGINEIRTIKIANNFSIAAEQETRNNDLIVGDYIIHNTYGKGLIIDMVDNVIEVKFENDKKIRKLSKTHHSIKKYEDDESLNS", "aa_hexdigest": "e6e2bc7dc17bb9e2d01b11770f6fad6b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0F3QEJ6", "query_cov": 0.9642857142857143, "subject_cov": 1.0, "identity": 0.677, "score": 244.0, "evalue": 1.45e-79, "valid": false, "product": "DNA 3'-5' helicase II", "uniref50_id": "UniRef50_A0A0F3QEJ6" }, "pscc": { "uniref50_id": "UniRef50_A0A0F3QEJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F3QEJ6" ], "product": "DNA 3'-5' helicase II" }, "genes": [], "id": "DFOKDJOJIG_1136", "locus": "GCA013367855_01135" }, { "type": "cds", "sequence": "contig_1", "start": 646029, "stop": 646211, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAATGTTTTCAATAAAGAAATTCATGATTTGTCTTATATTCCAACCATCGAAAATTATGAAGGCTTGTTTTTCTTTTTAAACAAGAAACACATTACTGATATAAATGCTTTTTTAGATCTGTTTAAAGATTTAGGAAAACAACACAATATTAATGGCTTAGAAGTTGTATATGAATGA", "aa": "MDNVFNKEIHDLSYIPTIENYEGLFFFLNKKHITDINAFLDLFKDLGKQHNINGLEVVYE", "aa_hexdigest": "2f4d50a3afdb6ea7115de8983dfb50be", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7118.981400000001, "isoelectric_point": 4.923245811462402 }, "id": "DFOKDJOJIG_1137", "locus": "GCA013367855_01136" }, { "type": "cds", "sequence": "contig_1", "start": 646805, "stop": 646999, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTTCGGTTAATCTAGAGGTTAGTTCTTCATCTATTCAAATGAATTTAACTCAAGGGGATACCAGAGAAATTATTGTTGAATCAATATCCTTAATTTCGAGACAAAAATTGAGTTTTGTTCCTTGTAGTTTAACAATTTTTTGGGATATGTCTTTTATTCTTTCTGCTGATAATAATTTTATTGATTGA", "aa": "MLGSVNLEVSSSSIQMNLTQGDTREIIVESISLISRQKLSFVPCSLTIFWDMSFILSADNNFID", "aa_hexdigest": "134f2509d5cb5e85eff095020be02482", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7143.107599999997, "isoelectric_point": 4.261810493469238 }, "id": "DFOKDJOJIG_1138", "locus": "GCA013367855_01137" }, { "type": "cds", "sequence": "contig_1", "start": 647185, "stop": 647340, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAAACTTATTCCAATAGATTTCTTTGATGCAGTAAATAAAAAGTGGGTAGATAGCCATAAAATCCCTGATTCTAAAACCTCTATAGGTTGCTTTGCGCAATTAGATAAAAATATTACTACATTAACTAAAAAAATATTAAATACATGA", "aa": "MNKKLIPIDFFDAVNKKWVDSHKIPDSKTSIGCFAQLDKNITTLTKKILNT", "aa_hexdigest": "e7879c837e3af9107b8cc9a726466674", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5820.7789999999995, "isoelectric_point": 9.519159889221193 }, "id": "DFOKDJOJIG_1139", "locus": "GCA013367855_01138" }, { "type": "cds", "sequence": "contig_1", "start": 647512, "stop": 647679, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTGAAATTATCTAGTTTAAACACCCCTTTAATTTTTTCAGTTTCAACAGATTTTAAAAACAGCGATGTTCAAACTCTTTATTTAGATGTAAACTCTACAATTCTACCAGACAAAAGTTTTTATACCGATCCCACCAAAAAAGAAAAATTTATTAATGTTTGA", "aa": "MELKLSSLNTPLIFSVSTDFKNSDVQTLYLDVNSTILPDKSFYTDPTKKEKFINV", "aa_hexdigest": "b56af5f0cd58fd824f2b67f4cbbcf863", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6287.101400000001, "isoelectric_point": 4.983893013000488 }, "id": "DFOKDJOJIG_1140", "locus": "GCA013367855_01139" }, { "type": "cds", "sequence": "contig_1", "start": 647812, "stop": 648486, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAAAATTTGCAACACTATTTCAACAAAAGAATTAGCCCAAAACATTAAAACAATAAACATAGAAAATATATTAAGACAATTAATTAAATGTTTCCCTGATGAAATAAATGCTATTGATCCAAATTCTATAAACATTGCAAAGTCTTTAACCAATGAAGAAAATTTTGAATATTATAAAGCAAGTTTAATTATTGATGCAATATTAACCTATGCACCTTATTTTGATTATTCAACAATTCAAATTGCTTCTGAATTTAATTTAGCAATAAACGGAAACAAAAAAATTGAATCAAAAGAAAAATATCAAATTAAACAAGCAAAACGTTTTTTTGATATTCCTTTTGGAACTTACTATGGTAAAACCTATTTTGGATTAAAAAACAAGCAAACAGTTGAACACATGATAAAAAGTATGATAGATGTTTATAAAGAACAACTAACAAAAAACTCTTGGTTAAGTAAGCAAACAAAAGAAAAAGCAATAACAAAACTTAACTCAATAGGTGTTTACGTTGGCTTTCCTGAAAAAATCCAAGATCACTATAAATATTTAATAGTTGAAGAATTTGATGGATACAATGATTTATTTGAAAATGTTTTAAAATTTAACAGAATTTTAGCAGAACATATCTTATCCGAATATGGAAAAACAGAAGACAAGAACCTTTGA", "aa": "MVKICNTISTKELAQNIKTINIENILRQLIKCFPDEINAIDPNSINIAKSLTNEENFEYYKASLIIDAILTYAPYFDYSTIQIASEFNLAINGNKKIESKEKYQIKQAKRFFDIPFGTYYGKTYFGLKNKQTVEHMIKSMIDVYKEQLTKNSWLSKQTKEKAITKLNSIGVYVGFPEKIQDHYKYLIVEEFDGYNDLFENVLKFNRILAEHILSEYGKTEDKNL", "aa_hexdigest": "da1324a1b1a56f1616594d17a8d461e9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26152.692500000026, "isoelectric_point": 7.639296913146972 }, "id": "DFOKDJOJIG_1141", "locus": "GCA013367855_01140" }, { "type": "cds", "sequence": "contig_1", "start": 648490, "stop": 648699, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACCTGCTATGGTTAATGCTTATTATAATCCTTCAAAAAATGTAATAGTATTCCCTGCTGCAATCTTGCAAAAACCTTTCTTTAGTGCAGATCAATCCTCTTCATCAAATTTCGGCGGAATTGGTGCTGTTATTGCCCATGAAATATCACACGGATTTGACAATAACGGAGCAAATTTCGACGAAAAAGGAAATATGATTAATTGA", "aa": "MSPAMVNAYYNPSKNVIVFPAAILQKPFFSADQSSSSNFGGIGAVIAHEISHGFDNNGANFDEKGNMIN", "aa_hexdigest": "6ebac6a6f366996c081c11a5317a49ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7351.097399999999, "isoelectric_point": 5.337602043151855 }, "id": "DFOKDJOJIG_1142", "locus": "GCA013367855_01141" }, { "type": "cds", "sequence": "contig_1", "start": 648745, "stop": 648915, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATTTATTTGAAGGTAGAGAAACATCTGTTGGTAAATGTAATGGAACATTAACCGTTTCAGAAAACATAGCAGATGCTGGTGGATTATCGTGTGCTTTAGCAGCAGCAAAATTAGAAAAAGATTATAACCCTAAAGATTTTTATATAAATTTTGCAACAATTTGA", "aa": "MINLFEGRETSVGKCNGTLTVSENIADAGGLSCALAAAKLEKDYNPKDFYINFATI", "aa_hexdigest": "8a342e6c8964b587198068ef7141ae43", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5986.715900000001, "isoelectric_point": 4.715044593811036 }, "id": "DFOKDJOJIG_1143", "locus": "GCA013367855_01142" }, { "type": "cds", "sequence": "contig_1", "start": 648949, "stop": 649089, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAACTATTGATTGTCACGCCCCTGCCAAATTAAGAACAAATGTTCAAATACAAAACTCTGATGATTTTTATACAACTTTCAACGTCAAAAAAGGCGACCCAATGTATTTGCCACCTGAAAAAAGAGTAAAAATTTGA", "aa": "MLTIDCHAPAKLRTNVQIQNSDDFYTTFNVKKGDPMYLPPEKRVKI", "aa_hexdigest": "b2da131b05c2e6442b6d66a0bc5641e4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5338.145600000001, "isoelectric_point": 8.931272697448733 }, "id": "DFOKDJOJIG_1144", "locus": "GCA013367855_01143" }, { "type": "cds", "sequence": "contig_1", "start": 649134, "stop": 650045, "strand": "-", "frame": 2, "gene": "tmcAL", "product": "nucleotidyltransferase", "db_xrefs": [ "COG:COG1323", "COG:J", "EC:6.3.1.22", "KEGG:K27343", "RefSeq:WP_175972343.1", "SO:0001217", "UniParc:UPI001589EFD5", "UniRef:UniRef100_UPI001589EFD5", "UniRef:UniRef50_A0A3M0A6W2", "UniRef:UniRef90_D1J8P3" ], "nt": "ATGAAAATAGGAATAATAGCTGAATTTAACCCATTTCACAATGGGCATATTTATTTGATTAATAAAATTAAAGAAATCTTCAAAGATCCTGAAATAATTGTTGCATTATCTTGCGATTATGTTCAACGTGGTGAAATAGCGTGTTTACCTTTTGAAGTTAGAAAAAACATTGCGCTTGAATATGGAGCAACAAAAGTTGTTGAATTAGATTTTTTTGCATCTACACAAGCGGCTCATATTTTTGCTAAAAAATCAATAGATTTGTTAATAAAAGAAGGTATTGATTATTTAGTTTTTGGGGTTTCTGACACCAATGATATAAAAAAATATTTAAACGCAGCAAACGTAATAGAGAAAAATTTTGATCAATATAACAAAGATGTAAGAATGAATTTAAAAACAGGAAAATCATATGTTTTGAGTTGTTTTTTGTCGCTAGAAAAATTAATAGGTGTTGAAAATATACCACAAGATATTTTGGGGTTTGAATATACCAAATACATAGTTTTTAATAATTTGTCAGTAAAACCGTTTTGCATAAAAAGAACAGCTCCACACAATTCATTAATAGCAAATAATAATTATGCTTCAGCAACAATGATACGAAAAATGTTAGAAAAAGGCGAAGATGTTTCAATGTTCACACCAATAAACATTCCTAAAAATTTTTCAAAAATTGAAGATAAATATATTGAATTTAAACAAAGGGTACAAAATTATTCTAAAGAAGAACTAGCAAATATTGCATTAGTATCCGAGGGAATGGAAAATCTTTTTAAAAAGAATATTGAAATAGCAAAAAATTATAATGAATTTGTTGATTTATGTACTTCAAAAAGATATACAAATAGTAGAATTAAACGAGTGATGTTGTACGTTCTTTTAGGAATAAAGAAAGAAGATTTGTGTTAA", "aa": "MKIGIIAEFNPFHNGHIYLINKIKEIFKDPEIIVALSCDYVQRGEIACLPFEVRKNIALEYGATKVVELDFFASTQAAHIFAKKSIDLLIKEGIDYLVFGVSDTNDIKKYLNAANVIEKNFDQYNKDVRMNLKTGKSYVLSCFLSLEKLIGVENIPQDILGFEYTKYIVFNNLSVKPFCIKRTAPHNSLIANNNYASATMIRKMLEKGEDVSMFTPINIPKNFSKIEDKYIEFKQRVQNYSKEELANIALVSEGMENLFKKNIEIAKNYNEFVDLCTSKRYTNSRIKRVMLYVLLGIKKEDLC", "aa_hexdigest": "9265b3b2ea512935c3fb72df84ea1351", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001589EFD5", "ncbi_nrp_id": "WP_175972343.1", "uniref100_id": "UniRef100_UPI001589EFD5", "db_xrefs": [ "SO:0001217", "UniParc:UPI001589EFD5", "RefSeq:WP_175972343.1", "UniRef:UniRef100_UPI001589EFD5" ] }, "ips": { "uniref100_id": "UniRef100_UPI001589EFD5", "uniref90_id": "UniRef90_D1J8P3" }, "psc": { "uniref90_id": "UniRef90_D1J8P3", "gene": "TmcAL", "product": "nucleotidyltransferase", "ec_ids": [ "6.3.1.22" ], "uniref50_id": "UniRef50_A0A3M0A6W2", "cog_id": "COG1323", "cog_category": "J", "kegg_orthology_id": "K27343" }, "pscc": { "uniref50_id": "UniRef50_A0A3M0A6W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3M0A6W2" ], "product": "tRNA(Met) cytidine acetate ligase" }, "genes": [ "tmcAL" ], "id": "DFOKDJOJIG_1145", "locus": "GCA013367855_01144" }, { "type": "cds", "sequence": "contig_1", "start": 650106, "stop": 650261, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA58", "UniRef:UniRef90_A0A454CA58" ], "nt": "ATGTTTAATAAAAAGAAAACAAGCAAGAAAAAACAAATAAAAAAAGAAAAAGAACAAAACGATATTGTGATGTTTGAAGATTTTGATTCAAACAAAAATTGTGAAGGCGATCAAAAAAATATTGAAATCCAAGAAACTGTGGAATTAAATTATTGA", "aa": "MFNKKKTSKKKQIKKEKEQNDIVMFEDFDSNKNCEGDQKNIEIQETVELNY", "aa_hexdigest": "8b8f169ff47a13e603d76fd76976f0cf", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 650046, "stop": 650261, "observations": { "mutation": [ 650108 ], "stop": [ 650108 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA58", "query_cov": 1.0, "subject_cov": 0.7183098591549296, "identity": 0.882, "score": 82.0, "evalue": 1.48e-19, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MLNKKKTNKKKQIKKEKEQSDIVMFEDFDSNKKSEDDQKNIEIQETVELNYWEDSSIEDFCDISLDELKKK" }, "paralog": false, "identity": 0.8873239436619719, "score": 127.0, "evalue": 3.88e-40, "description": "Internal stop codon at 650108. Nonsense mutation around 650108.", "psc": { "uniref90_id": "UniRef90_A0A454CA58", "uniref50_id": "UniRef50_A0A454CA58", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA58", "UniRef:UniRef50_A0A454CA58" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454CA58", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA58" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6156.8838, "isoelectric_point": 6.219856834411621 }, "id": "DFOKDJOJIG_1146", "locus": "GCA013367855_01145" }, { "type": "cds", "sequence": "contig_1", "start": 650482, "stop": 650691, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAATATTCAAGATACAATAGTTGCTATTTCTTCAGGTAATGTAAATCAAGCAATATCTATTATCAGATTATCTGGTGAAGATTCGATTGAAATTGTTAAAAAAGTTTTTAGTGGTAAAGTCGGAAAAGATAAAACAATTACTTACGGAAATATCATTGATAATATAACAGGGAAAATTGTTGATGAGGTATTAGTTAATTGA", "aa": "MKNNIQDTIVAISSGNVNQAISIIRLSGEDSIEIVKKVFSGKVGKDKTITYGNIIDNITGKIVDEVLVN", "aa_hexdigest": "d194803feaaad9dfd533e40e869ae95f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7432.4607, "isoelectric_point": 6.099699211120604 }, "id": "DFOKDJOJIG_1147", "locus": "GCA013367855_01146" }, { "type": "cds", "sequence": "contig_1", "start": 650803, "stop": 651558, "strand": "+", "frame": 1, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "COG:COG0486", "COG:J", "GO:0003924", "GO:0005525", "GO:0006400", "SO:0001217", "UniRef:UniRef50_A0A077L8N2", "UniRef:UniRef90_D1J8P4" ], "nt": "TTGGCAAATAGAGGAGAATTTACAAGGCGAGCATTTTTAAACGGAAAAATTTCATTAGATAAAGCCGAAGCGATAAACAGCTTAATTCATGCAAAAACAAATAAACAAGCAGAAATTGCTATTTCTCAATTTAATTCTAAGGACAATTTAATTATTGAAAATTTAATCAACGAACTACTTACTATAATTTCATTAATCGAAATTAATATTGATTATTCTGATTATAACGATATAAAAGAAATGGATGCTAACACCTTAACAAGTCAATTATCAAAATTTAATAGAAAATTAGATTCTATAATTAATAAATCAGAAAATGCTTGCGATATTTATAAAGGAATCAATGTTGCAATAGTAGGAAGACCCAACACTGGTAAATCATCACTTTTAAATTCTTTAATTGGTTCACAAAAAGCAATAGTAACAAATATACCCGGAACAACAAGAGACGTGGTAGAAGGTGAATTTCAGATTAATGGTTTTTTATTTAATTTAATTGATACAGCAGGTATAAGACAAACAAAAGATCTTGTTGAATCAATAGGTATCGAAAGATCTAAAAACGCAATAAAAGAAGCCAAAATTGTAATTCATATGCACGAACCATTTATTAAAGAAAATGAAGAAGATCAAATAATTAAAGAACTCTCAAAAGGTAAGGTATATATCCCAGTAATGAATAAGCAAGATCTTTTAAATAAAGATCAATATCCTAAGAACATGGTTTTAATCTCAAGCAAAGAAAATAGTCTATGA", "aa": "MANRGEFTRRAFLNGKISLDKAEAINSLIHAKTNKQAEIAISQFNSKDNLIIENLINELLTIISLIEINIDYSDYNDIKEMDANTLTSQLSKFNRKLDSIINKSENACDIYKGINVAIVGRPNTGKSSLLNSLIGSQKAIVTNIPGTTRDVVEGEFQINGFLFNLIDTAGIRQTKDLVESIGIERSKNAIKEAKIVIHMHEPFIKENEEDQIIKELSKGKVYIPVMNKQDLLNKDQYPKNMVLISSKENSL", "aa_hexdigest": "067c560be863f2b5f078405603c959e4", "start_type": "TTG", "rbs_motif": null, "genes": [ "mnmE" ], "truncated": "both", "pseudogene": { "start": 650503, "stop": 651828, "observations": { "mutation": [ 650689, 651556, 651739 ], "stop": [ 650689, 651556, 651739 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8P4", "query_cov": 1.0, "subject_cov": 0.5602678571428571, "identity": 0.9840000000000001, "score": 466.0, "evalue": 1.62e-162, "gene_start": 1, "gene_end": 251, "reference_start": 108, "reference_end": 358, "reference_sequence": "MKNNIQDTIVAISSGNVNQAISIIRLSGEDSIEIVKKIFSGKVGKDKTITYGNIIDNITGKIVDEVLVNWFIGNKNYTGENTVEINAHGGIVVTNKILNLLIANGARLANRGEFTRRAFLNGKISLDKAEAINSLIHAKTNKQAEIAISQFNSKDNLIIENLINELLTIISLIEINIDYSDYNDIKEMDANTLTNQLLKFNRKLDSIINKSENACDIYKGINVAIVGRPNTGKSSLLNSLIGSQKAIVTNIPGTTRDVVEGEFQINGFLFNLIDTAGIRQTKDLVESIGIERSKNAIKEAKIVIHMHEPFIKENEEDQIIKELSKGKVYIPVMNKRDLLNKDQYPKNMVLISSKENSLWELKNALVKNYLNIDLDDPQIIFNNRKLSLLKQAKISINTALESLSQGLGPEVVILDINEAWGSLSEILNKKHDNEALLDNIFSKFCLGK" }, "paralog": false, "identity": 0.9841269841269841, "score": 830.0, "evalue": 1.99e-308, "description": "Internal stop codon at 650689, 651556, 651739. Nonsense mutation around 650689, 651556, 651739.", "psc": { "uniref90_id": "UniRef90_D1J8P4", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "uniref50_id": "UniRef50_A0A077L8N2", "cog_id": "COG0486", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006400" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8P4", "UniRef:UniRef50_A0A077L8N2", "COG:COG0486", "COG:J", "GO:0003924", "GO:0005525", "GO:0006400" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L8N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L8N2" ], "product": "tRNA modification GTPase MnmE" } }, "id": "DFOKDJOJIG_1148", "locus": "GCA013367855_01147" }, { "type": "cds", "sequence": "contig_1", "start": 651589, "stop": 651741, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATATTGATTTAGATGACCCTCAAATAATTTTTAATAATAGAAAACTTTCGTTATTGAAGCAAGCGAAGATTTCTATCAATACTGCACTAGAATCACTAAGTCAAGGTTTAGGTCCTGAGGTGGTTATTTTAGATATAAACGAAGCTTGA", "aa": "MNIDLDDPQIIFNNRKLSLLKQAKISINTALESLSQGLGPEVVILDINEA", "aa_hexdigest": "2500abab43290ee70e2b4b9032c79911", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0F3QEF2", "query_cov": 1.0, "subject_cov": 0.9090909090909091, "identity": 0.62, "score": 69.7, "evalue": 7.25e-15, "valid": false, "product": "Catalytic cysteine-containing of GTPase, MnmE family protein", "uniref50_id": "UniRef50_A0A3B0P0P4" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5506.285699999999, "isoelectric_point": 4.385492134094237 }, "id": "DFOKDJOJIG_1149", "locus": "GCA013367855_01148" }, { "type": "cds", "sequence": "contig_1", "start": 651830, "stop": 652384, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAAAATAAACGACAAAATAGTTGTGAAAGCAACAGAACTAAGTTATGAAGGCTATGGGGTTGTAAGAAATGATAAACAAACACTTTTTGTTGAAAATCTCTTACCTGATGAAGAAGCAGAAGTAATAATTTATAAATCAAATTCAAAAATTGCTTATGGACGCACAATTAATTTGATAAAAAAATCAAGTAAAAGAGTTGAAGTTGTTGAGAAAGAATTATTTGAAAGTGGTGCTGCGCCATTGATGGTTTTAAGTTACGAAGATCAATTAAAATTCAAACAAGAAATTGTTAATAATTTAATAAAAAGAAATATTGGATTTAAAAACATTGAACAAATTCAACCCAGTCCAAAAATATTAAATTATAGAAATAAAATAACATTGCAAGTAAATTATAAAAACAACAATATTTATATTGGTTTCTATAAAAAAAATAGCAACAAACTAGTTGTTCAAAATTCGCTTCCTCTTTGTCATGATGAACTTGATAAATATTACAAAGAACTTGTTGGCACCTTAAAACAACAAATTTTATTAAAAGATAATTGA", "aa": "MYKINDKIVVKATELSYEGYGVVRNDKQTLFVENLLPDEEAEVIIYKSNSKIAYGRTINLIKKSSKRVEVVEKELFESGAAPLMVLSYEDQLKFKQEIVNNLIKRNIGFKNIEQIQPSPKILNYRNKITLQVNYKNNNIYIGFYKKNSNKLVVQNSLPLCHDELDKYYKELVGTLKQQILLKDN", "aa_hexdigest": "c747354f5d3104b0c4f4eba60270fa2f", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21425.54790000002, "isoelectric_point": 9.243041801452637 }, "id": "DFOKDJOJIG_1150", "locus": "GCA013367855_01149" }, { "type": "cds", "sequence": "contig_1", "start": 652493, "stop": 653161, "strand": "+", "frame": 2, "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "db_xrefs": [ "COG:COG2265", "COG:J", "EC:2.1.1.190", "GO:0008649", "KEGG:K03215", "SO:0001217", "UniRef:UniRef50_D1J8P5", "UniRef:UniRef90_D1J8P5" ], "nt": "TTGAAAAATAATTTGATTTTCTTATTGAAACCCAATGTAATTTCAATACAATGCCAGCACTCTCAAGAATCTAAAGATATATTCACTTCTTTATACAAATCAATGCCACAACAATTTAATATAAACAATAAAATATTCAATGTGAACAATGATTCTTTTTTTCAAGTAAATGAAGAACAAACTAGTTTAATATATAATCAAATTAAAAAATTCATAGATCAAGAAAATTCGGCAAAAATAATTGATGTTTATTCAGGTGTTGGTAGCATTGGAATATCTGTTTGTGGCAATGACTCTCAACTTGAATTAATTGAAATAAATAAATCAGCATGTGAGTGTGCAAAAAATAACATAAACCAAAATTTGATTAATCCAAAATTAGTGGATATAACCAATAGTTCAGCGCAAGACTTTTTTAATAAAAAAGAAGCAGAATATTTTGAAAAATCAATAGTTATATTTGATCCACCAAGAACGGGCTTAACAAATAAAGAAATTGACATGTTAGAAAAATGCAATAGTGTAATTTATATGAGTTGCAATCCTCATACAATGATTAGGGATTTAAAATATTTTGAAGAAAAAGGGTATAGCATTGAAAGGCTAATACCTTATGATATGTTTCCTCAAACTTATCATATTGAAATGTTGTCACTTTTAAAAAAATAA", "aa": "MKNNLIFLLKPNVISIQCQHSQESKDIFTSLYKSMPQQFNINNKIFNVNNDSFFQVNEEQTSLIYNQIKKFIDQENSAKIIDVYSGVGSIGISVCGNDSQLELIEINKSACECAKNNINQNLINPKLVDITNSSAQDFFNKKEAEYFEKSIVIFDPPRTGLTNKEIDMLEKCNSVIYMSCNPHTMIRDLKYFEEKGYSIERLIPYDMFPQTYHIEMLSLLKK", "aa_hexdigest": "9b9c0cc07151bc69215b4b0e07daf251", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rlmD" ], "truncated": "5-prime", "pseudogene": { "start": 652193, "stop": 653161, "observations": { "mutation": [ 652382, 652430 ], "stop": [ 652382, 652430 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8P5", "query_cov": 1.0, "subject_cov": 0.4134078212290503, "identity": 0.946, "score": 421.0, "evalue": 3.84e-144, "gene_start": 1, "gene_end": 222, "reference_start": 222, "reference_end": 443, "reference_sequence": "MYKINDKIVVKATELSYEGYGVVRSDKQTLFVENLLPREEAEVAIYKSNSKIAYGRTVNLIKKSCKRVEVVEKELFESGAAPLMVLSYEDQLKFKQEIVNNLIKRNIGFQNIEQIQPSPKILNYRNKITLHVNYKNNNIYIGFYKKNSNKLVIQNSLPLCHDELDKYYKELISALKQQILSKDNWIKELKLRQITLRHSNWSHNIEVIFIIENPNKKGFDLLKNNLIFLLKPNVISIQCQYIKESKDIFTSLYKSMPQQFNINNKIFNVNNDSFFQVNEEQTSLIYNQIKKFIDQENSTKIIDVYSGVGSIGISVCGNDSQLELIEINKSACECAKININQNLINPKLADITNSSAQDFFNKKEAEYFEKSIVIFDPPRTGLTNKEIDMLEKCNSVIYMSCNPHTMIRDLKYFEEKGYRIERLIPYDMFPQTYHIETVALLSKLDVDKHINIEIELDELDLTSAESKATYAQIKEYVWNKFELKVPTLYIAQIKRKCGIELREHYNKSKKEKQIIPQCTPEKEEAIMDALRHFKMIE" }, "paralog": false, "identity": 0.9409937888198758, "score": 612.0, "evalue": 2.29e-221, "description": "Internal stop codon at 652382, 652430. Nonsense mutation around 652382, 652430.", "psc": { "uniref90_id": "UniRef90_D1J8P5", "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "ec_ids": [ "2.1.1.190" ], "uniref50_id": "UniRef50_D1J8P5", "cog_id": "COG2265", "cog_category": "J", "kegg_orthology_id": "K03215", "go_ids": [ "GO:0008649" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8P5", "EC:2.1.1.190", "UniRef:UniRef50_D1J8P5", "COG:COG2265", "COG:J", "KEGG:K03215", "GO:0008649" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8P5" ], "product": "TRAM domain-containing protein" } }, "id": "DFOKDJOJIG_1151", "locus": "GCA013367855_01150" }, { "type": "cds", "sequence": "contig_1", "start": 653429, "stop": 653707, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGCCTTGGGTTATGTATCAAGCGCAATTGTTGCAAACGAAATAGGGAAACAAAATTTTGAAGCTGCTCATGAACTTGGAAATAGTGCATTAAAATTTGGCTTCTATGCACAGTCATTATTTTCGATATTAGGAGTTTCATTAACCTGGCCTTTGCTAAGACTTTATTCAATAGATATTCAAATAATTAATTCTGTTGGGTATGGTGTTATGGGAATTATGATGCTAAGAGCTGTTCTAGATATAGGAAACCTTACAACCCTAAGGGCTTTATGA", "aa": "MIALGYVSSAIVANEIGKQNFEAAHELGNSALKFGFYAQSLFSILGVSLTWPLLRLYSIDIQIINSVGYGVMGIMMLRAVLDIGNLTTLRAL", "aa_hexdigest": "c3030adf24607dd04495201ace3f42c0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9965.632099999992, "isoelectric_point": 6.526844215393066 }, "id": "DFOKDJOJIG_1152", "locus": "GCA013367855_01151" }, { "type": "cds", "sequence": "contig_1", "start": 653820, "stop": 653936, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAATTCCTGTTTCAAAAGCAATAGGAAGACCTAACTTAAGAATGTTTTTAACATATTTTGTGTTTCATGTTTCTTTAATTGAAATTTTATTGTTTTTGGTTATAAAGTGA", "aa": "MNQIPVSKAIGRPNLRMFLTYFVFHVSLIEILLFLVIK", "aa_hexdigest": "701afebf27eef300a3017b460235aed9", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4462.455099999998, "isoelectric_point": 10.277372550964351 }, "id": "DFOKDJOJIG_1153", "locus": "GCA013367855_01152" }, { "type": "cds", "sequence": "contig_1", "start": 654056, "stop": 654340, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATCACAACATCACTATAAATGATTTAGCTAAAATGAATCAATTTAATAAGGTGCTATACGGTGGAACTATAAACGGAATTACCCTAGTTAATACAAATCCTTTTAAGGTTTCAGTAACCTCTGAAACAACCATGGCTATAAAGTTCTTAAGAATAACCACTTGCGACATATTCATATATTCAATAGCTGTTATTTTTACAGCCAGTTTACAATCTGTTGAAAAAAATAAATTTTCAATATTTGGATTAATTACCAGTATATTTGTACGTGCAAGTTGA", "aa": "MKNHNITINDLAKMNQFNKVLYGGTINGITLVNTNPFKVSVTSETTMAIKFLRITTCDIFIYSIAVIFTASLQSVEKNKFSIFGLITSIFVRAS", "aa_hexdigest": "f00e39c404463e856b43497425ff05aa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10470.166999999992, "isoelectric_point": 9.730745124816895 }, "id": "DFOKDJOJIG_1154", "locus": "GCA013367855_01153" }, { "type": "cds", "sequence": "contig_1", "start": 654386, "stop": 654865, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAGAAAGAAAACAGTAAACAAAGAAATACAAATCGTTTAAATATTTTTATTGAAAAATCAAATGAATTATTTAAAGTTAAGCACTTTAAGTATATTTTCAAATTGACTTTACCAATCTTTTTTCAAACACTTTTTGTTGCTTTGGTATCGGTAGTTTCTTCTCTTGCTTCTTCGCATTATATAAAAGTCTATCACGCCGATGGTTCATACAACGGTTTTTATTTTTATATGATTGCAAAAATATTAACTGTTTACAAAATGATAACCTTTATTCCGATTATTTACCAAAGCGGAACATTAGTCTTGTGCTCAAATCTTTTTGGACAAAGCAAAATAAGAGACATACCAAAAGCTCTGTGGTCTGCCGTTTATGTATCATTAATAATAAATACTTGTGTTTATTTTATTATGTTTGGACTTGCGCCAATAGTTTTACAAGCTTCTGGAACAAAAAACATTCCTCTTTTTGGTTGA", "aa": "MRKKENSKQRNTNRLNIFIEKSNELFKVKHFKYIFKLTLPIFFQTLFVALVSVVSSLASSHYIKVYHADGSYNGFYFYMIAKILTVYKMITFIPIIYQSGTLVLCSNLFGQSKIRDIPKALWSAVYVSLIINTCVYFIMFGLAPIVLQASGTKNIPLFG", "aa_hexdigest": "63490f66bc6dd5f2951d092d8edc5458", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18261.521399999994, "isoelectric_point": 9.869481086730957 }, "id": "DFOKDJOJIG_1155", "locus": "GCA013367855_01154" }, { "type": "cds", "sequence": "contig_1", "start": 655157, "stop": 655549, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28", "PFAM:PF02881.24" ], "nt": "TTGCAAAACAAGCATAATAAAATTGACGAAGAATTTTTTGAAGATTTAGAAGAAATGCTGATTATGTCTGATATTTCGATTAAGCTAGTGCAAATAATTATTAACGAATGTAAAAAAGAAGTTAGAAATGAAAATATAACTGATCCAAAATTAATAAACGAGATAATTGCTGACAAACTGTTTACAATTTATACATCAAATTCTGTTGTCGATACAACACTTAATATTAAAGATGATAGGTTAAATGTAATATTGGTTGTGGGCGTCAATGGTTCAGGAAAAACTACATCAATATCAAAAATTGCTAAGAAATTAATTGATGAAGGTAAAAAAGTATTGATTGCTGCAGGTGATACATTTAGAGCAGCGGCAGTTGAACAATTAGAGATTTGA", "aa": "MQNKHNKIDEEFFEDLEEMLIMSDISIKLVQIIINECKKEVRNENITDPKLINEIIADKLFTIYTSNSVVDTTLNIKDDRLNVILVVGVNGSGKTTSISKIAKKLIDEGKKVLIAAGDTFRAAAVEQLEI", "aa_hexdigest": "16d8dd6e64880612f4fe0beda8f63d88", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 48, "aa_cov": 0.36923076923076925, "hmm_cov": 1.0, "evalue": 1.8742665601283022e-20, "score": 68.9959716796875, "start": 82, "stop": 129 }, { "id": "PF02881.24", "name": "SRP54_N", "length": 62, "aa_cov": 0.43846153846153846, "hmm_cov": 1.0, "evalue": 5.850048326301729e-15, "score": 50.97073745727539, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 14576.706099999976, "isoelectric_point": 4.94279842376709 }, "id": "DFOKDJOJIG_1156", "locus": "GCA013367855_01155" }, { "type": "cds", "sequence": "contig_1", "start": 655595, "stop": 656020, "strand": "+", "frame": 2, "gene": "ftsY", "product": "Signal recognition particle-docking protein FtsY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH26" ], "nt": "GTGGATCCAGCGGCTGTTGTTTATAGAGCAATCGATATAGCAAAATCAAAAAAATACGATATATTGATTGTAGATACTGCGGGAAGGTTGCAAAATAAAGTAAATTTAATGAACGAACTTGCCAAAATTAATAGGGTTTTAGCCTCTAAAATTCCTGATGCGCCTCATGAAAGTTTATTAGTTTTAGATGCAACAACCGGACAAAATGGTGTAATTCAGGCTAGGGTATTCAAAGAATCTACACCGCTGACCGGGATCATTCTAACAAAAATGGATGGAACCTCAAAAGGTGGAATTGTTTTAACAATCAAAGATGAGATTGGTTTGTTTGTAAAATACATTGGTTTAGGTGAAAAAGTTGAAGATTTAGCGGAATTTGATTTAGATTCTTATATCTATGGTTTAACAAAAGGTATTAACGAATAA", "aa": "MDPAAVVYRAIDIAKSKKYDILIVDTAGRLQNKVNLMNELAKINRVLASKIPDAPHESLLVLDATTGQNGVIQARVFKESTPLTGIILTKMDGTSKGGIVLTIKDEIGLFVKYIGLGEKVEDLAEFDLDSYIYGLTKGINE", "aa_hexdigest": "8f742c1b615280eef70971cd0e04ebab", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AH26", "query_cov": 0.9361702127659575, "subject_cov": 0.8407643312101911, "identity": 0.6970000000000001, "score": 189.0, "evalue": 1.68e-59, "valid": false, "product": "Signal recognition particle-docking protein FtsY", "uniref50_id": "UniRef50_A0A449AH26" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH26" ], "product": "Signal recognition particle-docking protein FtsY" }, "genes": [ "ftsY" ], "id": "DFOKDJOJIG_1157", "locus": "GCA013367855_01156" }, { "type": "cds", "sequence": "contig_1", "start": 656020, "stop": 656244, "strand": "+", "frame": 1, "gene": "ylxM", "product": "putative DNA-binding protein YlxM, UPF0122 family", "db_xrefs": [ "COG:COG2739", "COG:K", "KEGG:K09787", "RefSeq:WP_020002601.1", "SO:0001217", "UniParc:UPI00031E45B5", "UniRef:UniRef100_A0A2K9YTY8", "UniRef:UniRef50_C4XE46", "UniRef:UniRef90_A0A2K9YTY8" ], "nt": "ATGGAAACAATTGAAGAACGAGATTACTATGTAAAGTTGTTTGAAAAATATGGTGATTTATTACCACAATCTCAAAAACAAGCTCTACAATTACTTCTATATGAAGATTTGTCATATGGAGAATTAGCCCAAGAATTAGCGATGTCTAGACCAGGGGCTTACGACGCGGTTAAAAAAGCAAAATTACACTTAAAACAAATTGATGAAAAGTTAAAACAAAGTTAA", "aa": "METIEERDYYVKLFEKYGDLLPQSQKQALQLLLYEDLSYGELAQELAMSRPGAYDAVKKAKLHLKQIDEKLKQS", "aa_hexdigest": "78c4178b8a69d62122a90a1d2b675034", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00031E45B5", "ncbi_nrp_id": "WP_020002601.1", "uniref100_id": "UniRef100_A0A2K9YTY8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031E45B5", "RefSeq:WP_020002601.1", "UniRef:UniRef100_A0A2K9YTY8" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTY8", "uniref90_id": "UniRef90_A0A2K9YTY8" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YTY8", "gene": "YlxM", "product": "Predicted DNA-binding protein YlxM, UPF0122 family", "uniref50_id": "UniRef50_C4XE46", "cog_id": "COG2739", "cog_category": "K", "kegg_orthology_id": "K09787" }, "pscc": { "uniref50_id": "UniRef50_C4XE46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE46" ], "product": "Sigma-70, region 4" }, "genes": [ "ylxM" ], "id": "DFOKDJOJIG_1158", "locus": "GCA013367855_01157" }, { "type": "cds", "sequence": "contig_1", "start": 656344, "stop": 656511, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTATAAAAGAGTATTGATTAAACTTTCGGGTGAGGGTTTAGCAAATAAAGGTAAACACCTTGCAATTGATTATGAATTAGTTGAAAAATTTGCAAACCAATTAAAAACAATAATTAGTAGAAATATTGAAGTTGCGATTGTTGTCGGTGGCGGTAATTTTTGA", "aa": "MSYKRVLIKLSGEGLANKGKHLAIDYELVEKFANQLKTIISRNIEVAIVVGGGNF", "aa_hexdigest": "6d8fef6658cc5c79d62692c6239603c9", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6047.0342, "isoelectric_point": 9.52193202972412 }, "id": "DFOKDJOJIG_1159", "locus": "GCA013367855_01158" }, { "type": "cds", "sequence": "contig_1", "start": 656539, "stop": 657072, "strand": "+", "frame": 1, "gene": null, "product": "UMP kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "nt": "ATGCAAAGGGTTAGAGCAGATTATATTGGTATGTTAGCTACAACAATGAACGCGTTAGCTTTGCAATCAGGATTTGAGAGATTGAATTTGCATTGTAGAGTATTATCGAGCCTAACAATGGACCCAAAGGTTTGTGAAGTTTATATAAATGAAAAAGCTAAAAAATACCTAAGAGATGGAGAAGTTGTAATTTTTGCAGGAGGAACTGGACGTCCATTTTTTACAACCGATACAGCCTCAACATTATTCGCTAGTGAAATTGATGCGGATGCAATATTGATGGGAAAAAACAACGTTGATGGGGTGTATGATAGCGATCCAAAATTCAATAAAGACGCCAAAAAATTTCCAATCATTACATATGATGAATTACTAGAAAAGGAATTGAAAGTTATTGATCAAACCGCAGCATCAATGGCAAAGGAAAATAATATTGATATTTTAATATTTGATATTAATGAAGAAGATTCTTTAATAAGGGCAATAGAAAATAAAATACCTAACACAATAATAACTAGAGAAAGGGAAAAATAA", "aa": "MQRVRADYIGMLATTMNALALQSGFERLNLHCRVLSSLTMDPKVCEVYINEKAKKYLRDGEVVIFAGGTGRPFFTTDTASTLFASEIDADAILMGKNNVDGVYDSDPKFNKDAKKFPIITYDELLEKELKVIDQTAASMAKENNIDILIFDINEEDSLIRAIENKIPNTIITREREK", "aa_hexdigest": "9d37546a13ad5001476268f5f5e1a856", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P6F8", "query_cov": 0.96045197740113, "subject_cov": 1.0, "identity": 0.841, "score": 286.0, "evalue": 6.43e-97, "valid": false, "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_A0A3B0P6F8", "kegg_orthology_id": "K09903" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P6F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "product": "UMP kinase" }, "genes": [], "id": "DFOKDJOJIG_1160", "locus": "GCA013367855_01159" }, { "type": "cds", "sequence": "contig_1", "start": 657072, "stop": 657623, "strand": "+", "frame": 3, "gene": "frr", "product": "Ribosome recycling factor (Ribosome releasingfactor)(RRF)", "db_xrefs": [ "COG:COG0233", "COG:J", "RefSeq:WP_012855735.1", "SO:0001217", "UniParc:UPI0001BC24B0", "UniRef:UniRef100_D1J8P9", "UniRef:UniRef50_B3PLW1", "UniRef:UniRef90_D1J8P9" ], "nt": "ATGGAATTAGACAGTTATCTTTTAGAACTAGATGAAGAAATCAGCAAATCAATTGTTAACTATGAAAATCAATTAATGAAAGTTGTTATAGGTAGGGCCAATCCTGTTTTAATCAGTAAAATTAAATTAGAATATTATGGTTCACCAATTTCGGTAGAAGAAATAGCATCTATAACCACATCAGGACCATTACAATTAATTGTTAAGCCATATGATATGAGTGTGCTAAAAAATATTGAAAAAGCAATCATGGATAACAAGATAAACGTTTCAATAATAAATGAAGGACACCAATTGAGATTGCAATATCCTCAAATGACCACTGAAAAAAGAGTAGAAATGACAAAGCAACTTGCTTCAATAACCGAACAAGCAAGAATAGGCGTAAGACAAGCAAGACAAGATATCAATAAAAAAATAAAAGCAGATAGCGACCTTTCAGAAGACATTCAAAAACATTATCTAGATAATATTCAAAAAGAAGTTGATAAATCAATAGAAAAAATCAATGAAATGTCAAAAACAAAAGAAAAAGAACTTTTAACAATATAA", "aa": "MELDSYLLELDEEISKSIVNYENQLMKVVIGRANPVLISKIKLEYYGSPISVEEIASITTSGPLQLIVKPYDMSVLKNIEKAIMDNKINVSIINEGHQLRLQYPQMTTEKRVEMTKQLASITEQARIGVRQARQDINKKIKADSDLSEDIQKHYLDNIQKEVDKSIEKINEMSKTKEKELLTI", "aa_hexdigest": "6fac68c1362cb2287209923147a1e7b0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC24B0", "ncbi_nrp_id": "WP_012855735.1", "uniref100_id": "UniRef100_D1J8P9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC24B0", "RefSeq:WP_012855735.1", "UniRef:UniRef100_D1J8P9" ] }, "ips": { "uniref100_id": "UniRef100_D1J8P9", "uniref90_id": "UniRef90_D1J8P9" }, "psc": { "uniref90_id": "UniRef90_D1J8P9", "gene": "Frr", "product": "Ribosome recycling factor (Ribosome releasingfactor)(RRF)", "uniref50_id": "UniRef50_B3PLW1", "cog_id": "COG0233", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_B3PLW1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLW1" ], "product": "Ribosome recycling factor" }, "genes": [ "frr" ], "id": "DFOKDJOJIG_1161", "locus": "GCA013367855_01160" }, { "type": "cds", "sequence": "contig_1", "start": 657681, "stop": 657905, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACCTAAAAATTAGAGCAAGATCTGCAATTATATTATTTAGTATATTGATTCCACTACTTATTTTAACTTATTTTGTTAAAAGTTTAGGGGTGGCAATTTATATGTTTTTTAGCGCGTGGGCTTTGTATGAAGTTATTTCACACAACCAAAACAAAAGATCTGTTAATTTAGTTATATCCTTACTATCAATATTAATTTTAATAATACCAGTTAATTGA", "aa": "MKNLKIRARSAIILFSILIPLLILTYFVKSLGVAIYMFFSAWALYEVISHNQNKRSVNLVISLLSILILIIPVN", "aa_hexdigest": "2a526fcf00d7833488bb829bc71b9ef9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8432.250899999995, "isoelectric_point": 10.377040672302243 }, "id": "DFOKDJOJIG_1162", "locus": "GCA013367855_01161" }, { "type": "cds", "sequence": "contig_1", "start": 658392, "stop": 658634, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTAATTTTGGAAATTCCGATTCAATAAAAATATTAATAAATCAAAAGCAAATTATTACGGCCGTTTTTGTTCTTCCTGCAATTTCTATTATTGGCGATTTAGTATTTTCGGGAATAAAAAGAGCTCAAAATATTAAAGATTTTTCTGAATTAATAGCGGGTCATGGAGGAATTATGGACAGATTTGATTCGCTATCGTTTAATACGGTATTTTTAACTTTTATATTAATGATTTAA", "aa": "MGINFGNSDSIKILINQKQIITAVFVLPAISIIGDLVFSGIKRAQNIKDFSELIAGHGGIMDRFDSLSFNTVFLTFILMI", "aa_hexdigest": "3abe1bc2c0a1005faea91ebe37609cbc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8790.275099999995, "isoelectric_point": 6.520194053649903 }, "id": "DFOKDJOJIG_1163", "locus": "GCA013367855_01162" }, { "type": "cds", "sequence": "contig_1", "start": 658737, "stop": 659258, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATAAGACTTTTGTGCAATTATGCAAAGAAATAAAATTTGAATTTGATGAACAACAATTTTATGGTGCAAAAGTTTTGAGTGTTGAATCAAACTTAAATGAATGGAAATTTAAGATTGCCTTTGCAAGCCATATTGACATTGAACAATACAAACAATTTAGAGAAAAATTAGTTAAAAGATTTGGCAAGGTTGAATTAATCTTGGCTATTGAGACAATTATAAGAAATAAACAAATTATTGTTGATTATTTAAATTATCTAATAGAATGTAACCCCAAGAAATATTGGCCTTTTGTGAAATATAATTATTCATGTATAACTACTTCAACAATTGAAATAGATGAAAAAACATTAACACTTTCCTTACTAACAAATGAAATTTATGAAGTTTTTAAAAGTAATAAAGATCTTTTTAAAGAAGCGCTTGCTTCAATAGGATTTGATTTTTATAATGTAGTTTTTAATTTGGGATTGAATAATAAAAAAAGCGTTGCAAGCAAAAAACTAGATGGTTGA", "aa": "MLDKTFVQLCKEIKFEFDEQQFYGAKVLSVESNLNEWKFKIAFASHIDIEQYKQFREKLVKRFGKVELILAIETIIRNKQIIVDYLNYLIECNPKKYWPFVKYNYSCITTSTIEIDEKTLTLSLLTNEIYEVFKSNKDLFKEALASIGFDFYNVVFNLGLNNKKSVASKKLDG", "aa_hexdigest": "066bdd68cc72a590683ca23d61317a52", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20375.390200000005, "isoelectric_point": 8.278788185119634 }, "id": "DFOKDJOJIG_1164", "locus": "GCA013367855_01163" }, { "type": "cds", "sequence": "contig_1", "start": 659361, "stop": 660431, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGGATTTAACTACGGCAAACGAAACGTTTGAAGAAATAAAAATAACCACAAAAGGAGAAGTTTTTTTCATTGAAAGCAAAGTGCTAAAAAACAAAAAAATTCTATATACGCTAAAGATTTCAGATTATAAAGAAGCTATTCTTGTTAAAAAATTTTATGATTCCGAAAACGAAATACCTGATATAAAGGTAGGGGACACGATTATTGTTACTGGAAAATTAAGTGATGATAATTTTACAAAATCAAAAGTTTTAATAGCAATGGGCAAGGAATGCTTCATAAAGACAGAACCATTACAAAAACTTATTGAAGATAACGAAGACAATAAAAGAGTTGAATTAGCCGCAAGATCTAACATGAGTGCTCAAGATGGAATTTCAACGCCCACCGACTATATTAATGCTGCTAAATTTTATGGTCATAAGGCTATAGCATTGACCGACCTAGATGATGCGCAAGGCTTTCCCGAATTTTATAATTATGCCAAAAAGCAAAAGGATGTTAAGCCTATATACGGCGCAACATTAAGCACCGTTGATTCAGATAAAAAATTCTTCTACAACTTTAAAAATTTCAACTTAAAAGAAGAAGAATACGTTGTATTTGACCTTGAAACAACAGGATTGAGTCCAAGATTGGATGACATTATTGAATTTGGTGCCACTATTATAAAAAATAATACTATTACTCAAAAAATTCAATTCTTTTTGCAAACGAAACGTGAACTATCAGCCTTTACTGTTGAAAGCAGCCACATTAATAATGAAATGTTAAAGAACGGTTATGCACAAGAAGAAGGCATTAAAAAGATTTATGAAATATTAAAGGATAAAATTGCAGTTGCTCACAATGCAAACTTCGATATAAATTTTTGCAAAGAAAAATTTAGCAAACTTGGCCTTGATAATTCTAAAATTTTTGGCCTTGATACATTGGCCTTATCTTATTTTTTAAATCCCGATGAATTTCGTCATTCTTTGGGTTATGTTGCTAAAAGATTCAATATTATTTATGATACAACTGTTGCCCACCGTGGTGATTATGACGCTAACGTTTTGGCTAAGATTTGA", "aa": "MDLTTANETFEEIKITTKGEVFFIESKVLKNKKILYTLKISDYKEAILVKKFYDSENEIPDIKVGDTIIVTGKLSDDNFTKSKVLIAMGKECFIKTEPLQKLIEDNEDNKRVELAARSNMSAQDGISTPTDYINAAKFYGHKAIALTDLDDAQGFPEFYNYAKKQKDVKPIYGATLSTVDSDKKFFYNFKNFNLKEEEYVVFDLETTGLSPRLDDIIEFGATIIKNNTITQKIQFFLQTKRELSAFTVESSHINNEMLKNGYAQEEGIKKIYEILKDKIAVAHNANFDINFCKEKFSKLGLDNSKIFGLDTLALSYFLNPDEFRHSLGYVAKRFNIIYDTTVAHRGDYDANVLAKI", "aa_hexdigest": "95cdb3982d582bffb74e9a0c0145f9a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 143, "aa_cov": 0.3960674157303371, "hmm_cov": 0.8321678321678322, "evalue": 2.178481429876677e-08, "score": 29.88019371032715, "start": 201, "stop": 341 }, { "id": "PF00929.31", "name": "RNase_T", "length": 165, "aa_cov": 0.4410112359550562, "hmm_cov": 0.9878787878787879, "evalue": 3.720075447875519e-32, "score": 107.76918029785156, "start": 200, "stop": 356 } ], "seq_stats": { "molecular_weight": 40668.8084, "isoelectric_point": 5.606109428405762 }, "id": "DFOKDJOJIG_1165", "locus": "GCA013367855_01164" }, { "type": "cds", "sequence": "contig_1", "start": 660438, "stop": 660701, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGACAAACTTGCAAAAGATTACAATATCTTGAACTCACAGGATTTAAACAATGTTGATAACCAAAAAATACTTTCCCATAAATTTACATACGAAACAAGAATATTAGTAAAAAATCAAGCCGGCCTTAAAAAATTATTCAAAATTATTTCGCGTTCTTTAACTTCCCAATTTAAAAAAAATCCACAAATTTACTTTGATCAATTGGGTAATGATGAAGATTTGTTTTTTGGATCAGCAACAAATCAATCATATGTTTGA", "aa": "MIDKLAKDYNILNSQDLNNVDNQKILSHKFTYETRILVKNQAGLKKLFKIISRSLTSQFKKNPQIYFDQLGNDEDLFFGSATNQSYV", "aa_hexdigest": "1a44ea6cd0439bd8cf14724c94a103f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10120.379899999993, "isoelectric_point": 9.222669792175292 }, "id": "DFOKDJOJIG_1166", "locus": "GCA013367855_01165" }, { "type": "cds", "sequence": "contig_1", "start": 660732, "stop": 661310, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAAATTCACTAAAATATTATGACTATATTGAATTACCCCCTATATCATCATTTCAATATATGATTGATAGTAACCACGTTACATTAGATGAATTAAAATTTTTGTATAAAGATTTAATTTCAAAAGCAAAAAGTTTAAACAAAATGTGCGTTGCTGTTTCTGATTGCAGATATATTTATAATTATCAAAGATTAATTCATGAAATTTATATTGAGGCTCCAACTTTGGGGGGTGGGCTTCATTGGCTTAAGAAGAGACCATCTCATCCAGAATTTAGATATTTAACAACAAGAGAGATGTTAAAAGAATTTGCTTTTTTGAATGATAGTTATTTAATTAATGAAATAGTAATTGAAAATCCAAACAAAATTGCTAATGAAATTTCATCTAATATTCAAGTTATTAAAGATAAATTGTATGTTCCAACTTTTGATAACTCACCTCAAAAATTAAGAGAAGTTGTTTATAGGACTTTAAAAGAAAGATACGGGGATAATCCCGATCCCATCATAGTTAAAAGAATTGAAAAGGAATTAAATCCAATTATAAAGTATGGATATTCTGTTATTTATTGA", "aa": "MLNSLKYYDYIELPPISSFQYMIDSNHVTLDELKFLYKDLISKAKSLNKMCVAVSDCRYIYNYQRLIHEIYIEAPTLGGGLHWLKKRPSHPEFRYLTTREMLKEFAFLNDSYLINEIVIENPNKIANEISSNIQVIKDKLYVPTFDNSPQKLREVVYRTLKERYGDNPDPIIVKRIEKELNPIIKYGYSVIY", "aa_hexdigest": "3b2a3943ebcbe6e616d5204578438495", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22758.07350000002, "isoelectric_point": 8.329589271545409 }, "id": "DFOKDJOJIG_1167", "locus": "GCA013367855_01166" }, { "type": "cds", "sequence": "contig_1", "start": 661503, "stop": 661781, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023B0427D", "PFAM:PF07733.18" ], "nt": "TTGCCAGACAAGCATTGTCCAAATTGCAACTCAATATTGTCAAAAGATGGGCATAATATCCCTTTTGAAACATTCTTGGGATTTGAAGCGGATAAAGTTCCTGATATTGATTTGAATTTTAGTGGTGAATATCAACCAACAATTCACAATTATGTTAAAGAATTGTTTGGAGAAGCGCATACATATAGAGCTGGTACAATAGCAACCGTAGCAGAAAAAACAGCATATGGATTTTGCAAAAAATATGAAGAAGAAAAGAGAGGAAATTATCAACCTTGA", "aa": "MPDKHCPNCNSILSKDGHNIPFETFLGFEADKVPDIDLNFSGEYQPTIHNYVKELFGEAHTYRAGTIATVAEKTAYGFCKKYEEEKRGNYQP", "aa_hexdigest": "e91ce885ba96cabbf188544f2aa38639", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00311D31FE", "query_cov": 0.8695652173913043, "subject_cov": 0.9523809523809523, "identity": 0.688, "score": 123.0, "evalue": 5.56e-35, "valid": false, "uniref50_id": "UniRef50_UPI0023B0427D" }, "pscc": { "uniref50_id": "UniRef50_UPI0023B0427D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023B0427D" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 59, "aa_cov": 0.6413043478260869, "hmm_cov": 1.0, "evalue": 1.452259998727377e-19, "score": 66.29480743408203, "start": 18, "stop": 76 } ], "seq_stats": { "molecular_weight": 10477.550399999993, "isoelectric_point": 5.471401023864746 }, "id": "DFOKDJOJIG_1168", "locus": "GCA013367855_01167" }, { "type": "cds", "sequence": "contig_1", "start": 661788, "stop": 661937, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07733.18" ], "nt": "TTGTTCTTGGACTTTTTAGCATCTAAAACACAAGGAGTAAAGAGAACAACTGGACAACATCCAGGAGGAATTATTATTATTCCTAAAGAATTCGATGTTGAGGATTTTACGCCTGTTAACTATCCTGCTAACGACACAGAAAGTTCTTGA", "aa": "MFLDFLASKTQGVKRTTGQHPGGIIIIPKEFDVEDFTPVNYPANDTESS", "aa_hexdigest": "b044285cd8dda354983023088a65d810", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 41, "aa_cov": 0.8367346938775511, "hmm_cov": 0.9512195121951219, "evalue": 2.4124699775641714e-09, "score": 32.7967643737793, "start": 6, "stop": 46 } ], "seq_stats": { "molecular_weight": 5412.988600000002, "isoelectric_point": 4.646496772766113 }, "id": "DFOKDJOJIG_1169", "locus": "GCA013367855_01168" }, { "type": "cds", "sequence": "contig_1", "start": 661992, "stop": 662273, "strand": "+", "frame": 3, "gene": null, "product": "DNA polymerase III polC-type", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PG80" ], "nt": "TTGTTGGGTCATGATGATCCTACCGTTATTAGAATGCTTGAAACATTGACAAATACAAACGCAAAGGAAATACCAAAATTTGATGCAGAAGTTATGAAACTTTTTTATACAACTGAATCATTGAAAATTAGTCCTTCAGACATTGGGGGCGAAACAACCGGGGCATATGGGTTGCCTGAATTTGGAACAAACTTTGTAAGAAAAATGTTAAAAAAAGCTCAACCGAGATCGTTTAATGATTTAATTCTTCTTTCGGGGTTGAGTCATGGTACGGATGTTTGA", "aa": "MLGHDDPTVIRMLETLTNTNAKEIPKFDAEVMKLFYTTESLKISPSDIGGETTGAYGLPEFGTNFVRKMLKKAQPRSFNDLILLSGLSHGTDV", "aa_hexdigest": "2d0c3d1ead0a0adca86ab0ead46ba3d3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FBH3", "query_cov": 1.0, "subject_cov": 0.9117647058823529, "identity": 0.624, "score": 118.0, "evalue": 6.6e-33, "valid": false, "product": "DNA polymerase III alpha subunit finger domain-containing protein", "uniref50_id": "UniRef50_A0A3B0PG80" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PG80" ], "product": "DNA polymerase III polC-type" }, "genes": [], "id": "DFOKDJOJIG_1170", "locus": "GCA013367855_01169" }, { "type": "cds", "sequence": "contig_1", "start": 662637, "stop": 662825, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAGTGGCGCAGCAAGCGTTCACCAAAAACTTATTGAATTAAAAAATCGCCAAAATTCAACTGGCGAAAAATTATCTGTAAAGGAAGAAGCATTAATTCCTGTTTTAGAAATTACTCAAGAATTATACGCAAGGGGATTTAAAATTAAAAATGTTGATTTAAAACTTTCTTTGGCTGATAAATGA", "aa": "MSSGAASVHQKLIELKNRQNSTGEKLSVKEEALIPVLEITQELYARGFKIKNVDLKLSLADK", "aa_hexdigest": "b4b10474809773a2b317a662a22fcc8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6881.9046, "isoelectric_point": 9.09998645782471 }, "id": "DFOKDJOJIG_1171", "locus": "GCA013367855_01170" }, { "type": "cds", "sequence": "contig_1", "start": 662871, "stop": 663050, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14579.12" ], "nt": "GTGGATGGTTTAGGAATTACTGTTGCTCAAACAATAGTATCTGCAAGAAAAGAAGGCTTTTTCCTTTCAATTGAAGACCTTGCTTCGAGAACAAAAGTCAATTCAAGAATTATTTCGGAATTAAAAAAAATAGGCGTTTTAGATTGCTTAGATGATACCAATCAAGTAAGTTTGTTTTAA", "aa": "MDGLGITVAQTIVSARKEGFFLSIEDLASRTKVNSRIISELKKIGVLDCLDDTNQVSLF", "aa_hexdigest": "9f00ef4ff60f338034dae1f1abda16da", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14579.12", "name": "HHH_6", "length": 56, "aa_cov": 0.8813559322033898, "hmm_cov": 1.0, "evalue": 7.160964536253465e-11, "score": 37.870018005371094, "start": 2, "stop": 53 } ], "seq_stats": { "molecular_weight": 6457.3866, "isoelectric_point": 5.086430549621581 }, "id": "DFOKDJOJIG_1172", "locus": "GCA013367855_01171" }, { "type": "cds", "sequence": "contig_1", "start": 663303, "stop": 663479, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA64", "UniRef:UniRef90_A0A454CA64" ], "nt": "ATGCTTTTGAAGAAAAATATTATTAAATATAAATTTACCCCTTTGGTTTTGCAAACACACAAATACGAAATAACATTTTTAAGAAAGGATTTACAAAAACCTAAAATATATTCAATAAGACAACAAAAACAAATTAAAGCAACTACACAAAAATTTTTTTGTATAAGATTTCTTTGA", "aa": "MLLKKNIIKYKFTPLVLQTHKYEITFLRKDLQKPKIYSIRQQKQIKATTQKFFCIRFL", "aa_hexdigest": "41e5563b649c1ac03ca1dd957d972dbb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CA64", "query_cov": 0.9655172413793104, "subject_cov": 0.8484848484848485, "identity": 0.982, "score": 109.0, "evalue": 1.8e-30, "valid": true, "uniref50_id": "UniRef50_A0A454CA64" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA64" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7161.656199999999, "isoelectric_point": 10.392964363098145 }, "id": "DFOKDJOJIG_1173", "locus": "GCA013367855_01172" }, { "type": "cds", "sequence": "contig_1", "start": 663713, "stop": 664000, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGAAATTGGAATTAGAAAGGTTAATGCTTCAGTGTTAAATACCTTAAATTTAAAAACTAACAATAAAACAATAATAATTTGTATTGCAGCAATTGCTAATGCTATTGGAAACTCAACACCTCATCTTGATAAATTTTTTGTTGATTTTATAATTCAACCATCTATGCCTAAAATTAAAAACATTATAAAAGAAGGAATGACAATTAAAAATAAACCCGTTAGAGCTATTTTTCATGAACGTGACAATTCTCTTTTTGGCTTTCCTGATTCTACAACATAA", "aa": "MAKEIGIRKVNASVLNTLNLKTNNKTIIICIAAIANAIGNSTPHLDKFFVDFIIQPSMPKIKNIIKEGMTIKNKPVRAIFHERDNSLFGFPDSTT", "aa_hexdigest": "68b5c6f1d8ce42c76e84c1234f52ebb4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10553.310799999988, "isoelectric_point": 9.843113517761228 }, "id": "DFOKDJOJIG_1174", "locus": "GCA013367855_01173" }, { "type": "cds", "sequence": "contig_1", "start": 664231, "stop": 665487, "strand": "+", "frame": 1, "gene": "glyA", "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "COG:COG0112", "COG:E", "EC:2.1.2.1", "KEGG:K00600", "RefSeq:WP_044420793.1", "SO:0001217", "UniParc:UPI0005C81B46", "UniRef:UniRef100_UPI0005C81B46", "UniRef:UniRef50_P56089", "UniRef:UniRef90_D1J8Q3" ], "nt": "ATGTATAAAAAAATAAGTTTAGATGATAAAGAAATCGAAGAATTAATTAACCTAGAAAATCAACGTTTAGAAGACCATGTAGAACTAATAGCATCTGAAAATTATGTTTCAGAAGATGTTATGAAGGCAAATGGTTCTTGTTTAACTAATAAATATGCAGAAGGCTATCCTAATAAAAGATATTATGGTGGTTGCGAATATGTTGATAAAATAGAAGAAATTGCACAAGAAAGAGCAAAAAAATTATTCAATGTAAAATATGCTAATGTTCAACCATATTCAGGAAGTGTTGCAAATGCTGCTGTATACATGGCAATGGTTGATCCTGGAGATAAAGTCTTAGGATTAGATTTAAATTCAGGAGGACACTTATCACACGGATATAAAATTTCGTTTAGTGGTAAATTTTACGAAGCACATACCTATTCTGTTAACGATCAAGGTTTATTGGATTACGACAAGATATTGGAAATTGCAAAAGAAGTAAAACCAAAAATGATAATTTGTGGATATTCTGCATATTCTCAAATTGTTGACTTTGCTAAATTTAGAAAAATTTGCGATGAAGTAGGTGCAAAATTATTTGCCGATATTTCTCATATATCAGGATTAATAATAGCAGGATTACACCCATCGCCTTCGGGTTATGCTGATGTTATAATGACTACAACACATAAGACATTGCGTGGCACTCGTGGAGCAATAATTTTAACTAATGACGAAGAAATTTTTAAAAGAGTTAACAGTGCGGTTTTTCCTGGCGTTCAAGGAGGACCTTTGTTGCACCAAATAGCGGCAAAGGCAGTAAGCTTTAGAGAAGCATTGCAACCAGAGTTTATTGATTATCAAAAGCAACTATTGTTGAATTCAAAAATTATGTGTCAAACATTTTTGAACAAGGGAGTAAAAATTATCTCTGGCCTTACTCAAAATCATTTATTTATGATTGATGTAAAAACTAGTTATGGAATAACAGGAAAATATGCAACAGAAGTTTTGGGTAGAGTTAATATAACCGTGAATAAAAATACTATTCCAAACGATAGTGAAAAACCAACCGTTGCTAGTGGAATAAGAATAGGATCTGCGGCAATGACATCAAGAGGATTAAAGGAAGATGAGTTCATCATTTTAGCCAATTTAATTGATAAAGTTCTAAGAGATCCAGAAAACGAAAACTTATTATATGTAGTAAAGAAAGAAATTGCAAAATTAACGAATTCATTCCCAATTAAACGTTCTCATTTAGTTCGTTAG", "aa": "MYKKISLDDKEIEELINLENQRLEDHVELIASENYVSEDVMKANGSCLTNKYAEGYPNKRYYGGCEYVDKIEEIAQERAKKLFNVKYANVQPYSGSVANAAVYMAMVDPGDKVLGLDLNSGGHLSHGYKISFSGKFYEAHTYSVNDQGLLDYDKILEIAKEVKPKMIICGYSAYSQIVDFAKFRKICDEVGAKLFADISHISGLIIAGLHPSPSGYADVIMTTTHKTLRGTRGAIILTNDEEIFKRVNSAVFPGVQGGPLLHQIAAKAVSFREALQPEFIDYQKQLLLNSKIMCQTFLNKGVKIISGLTQNHLFMIDVKTSYGITGKYATEVLGRVNITVNKNTIPNDSEKPTVASGIRIGSAAMTSRGLKEDEFIILANLIDKVLRDPENENLLYVVKKEIAKLTNSFPIKRSHLVR", "aa_hexdigest": "b8c5082caf53703babeccbbbd683622a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0005C81B46", "ncbi_nrp_id": "WP_044420793.1", "uniref100_id": "UniRef100_UPI0005C81B46", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C81B46", "RefSeq:WP_044420793.1", "UniRef:UniRef100_UPI0005C81B46" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C81B46", "uniref90_id": "UniRef90_D1J8Q3" }, "psc": { "uniref90_id": "UniRef90_D1J8Q3", "gene": "GlyA", "product": "Serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_P56089", "cog_id": "COG0112", "cog_category": "E", "kegg_orthology_id": "K00600" }, "pscc": { "uniref50_id": "UniRef50_P56089", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P56089" ], "product": "Serine hydroxymethyltransferase" }, "genes": [ "glyA" ], "id": "DFOKDJOJIG_1175", "locus": "GCA013367855_01174" }, { "type": "cds", "sequence": "contig_1", "start": 665498, "stop": 665794, "strand": "+", "frame": 2, "gene": null, "product": "Asp23/Gls24 family envelope stress response protein", "db_xrefs": [ "RefSeq:WP_012855740.1", "SO:0001217", "UniParc:UPI0001BC24B5", "UniRef:UniRef100_D1J8Q4", "UniRef:UniRef50_A0A3G8LGV6", "UniRef:UniRef90_D1J8Q4" ], "nt": "ATGATATTAGCGGAAATAAAAAATTTAATTAAATCTTCAATAAGTATTATTCCAGGAATAGTTGATTTTGTTGAATTAACTTCAACAAATAATATAAATATTGAAAATGATGGTTTAATCATTGAGGAAGATCCACAAAATAAAAACATTATAAATATAACAATTGGTTTGAGAATAATGAGCAATTTAAGTGCTAAAAATATCGTTGAAGGAATATATGAAATCATTCCATATAACTTAACTTCTTACGATATAAAATTAGGAACATTAACAATATATATTAAAGGAACTAAATAA", "aa": "MILAEIKNLIKSSISIIPGIVDFVELTSTNNINIENDGLIIEEDPQNKNIINITIGLRIMSNLSAKNIVEGIYEIIPYNLTSYDIKLGTLTIYIKGTK", "aa_hexdigest": "7f63b54d2cc3e32b5b5d28cdfbd465c3", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001BC24B5", "ncbi_nrp_id": "WP_012855740.1", "uniref100_id": "UniRef100_D1J8Q4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC24B5", "RefSeq:WP_012855740.1", "UniRef:UniRef100_D1J8Q4" ] }, "ips": { "uniref100_id": "UniRef100_D1J8Q4", "uniref90_id": "UniRef90_D1J8Q4" }, "psc": { "uniref90_id": "UniRef90_D1J8Q4", "uniref50_id": "UniRef50_A0A3G8LGV6" }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LGV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGV6" ], "product": "Asp23/Gls24 family envelope stress response protein" }, "genes": [], "id": "DFOKDJOJIG_1176", "locus": "GCA013367855_01175" }, { "type": "cds", "sequence": "contig_1", "start": 665923, "stop": 667422, "strand": "+", "frame": 1, "gene": "fakA", "product": "DhaL domain-containing protein", "db_xrefs": [ "COG:COG1461", "COG:I", "SO:0001217", "UniRef:UniRef50_N9TTI7", "UniRef:UniRef90_D1J8Q5" ], "nt": "ATGTCATCTACAATTTCTTCTGCATGTAATTCTGAATGCTTAGAAGACGATTGCAGTATTGAATCAGCTTCGAGAATTGTTGCTCAAAATATGTTAATGTCCGCCAGAGGAAATTCTGGTGTAATTTTAAGTCAAATTTTTAAAGGTTTTTCTAATGGGTTTGCATCTAAAAAGTCTGCTAATGCTTTTGATTTAGTTAATGCTTTCGAAATGGCTGCTAAATCAGCTTATGGTGCAGTTTTAAAACCTGTTGAAGGAACTATTTTAACCGTTATTAGAGAAGTTTCTGAAGGTTTAAAAAACCAAGTTGTGGTTGATACTACTATTGAAGAAGTTTTTGAAATTGCTGTTAAATTAGCAAGAAAAAGTTGTGATGGTACTCCTAAATTATTAAAAATATTAAAAGAAGTAGGTGTTACTGATTCAGGTGGTGAAGGTTTATATGCAATATTGCAGGGAATGAGCGCATACTTTAGTGGTAATAAAGTAGAACCTATTGTTGCAGAAGAGTCGGTTGAAAAATTTATTTCTGATGAAGAGGTATATGAAGGCGAATTTGGATATTGCACCGAATTTATTTTGGAATTATATAAACCAAAAAAATTCGACAAAGATTATTTAGTTCGTAAACTAGAAAAAATTGGTAATTCAATGGTTGTGGTAAATGATGACAAAATTTTAAAAGTTCATATTCATACCCCTAAGCCCGGTGATGTTTTAAATAGCGTAAATTCTTTGGGACAATTTGCAAAAATTAAAATAGATAACATGACTTTACAAGCAAACAATAGTAAGAAAACTGCAAAGAAAAAACAAACCGAAGCGCCAAACAATGTTGAATTAAAAAATAGCGCCATTGTTTCGTGCAATACAGGCCAAGGAATCGTAACCTTAATGAAAGAAAACGGTGTTTCATATGTTGTTGATGGTGGTCAAACAAACAACCCCTCAATTCAAGATATTGTTAATGCAATAAATGCAGTCAATGCAAAAACGGTATTTGTATTGCCAAATAACTCAAATATAATTTTATCTGCTCAACAAGCCGGTACTATTATTAGAGATAAGAATGTAATTATTATTCCAACTAGAAGTCAAGTTCAAGGATTAAATGTTGCATTGAATTATAGTTCTGAAAATAGTGATGAAGAAAATCATGAATTAATGAGTAACAGTTTAAAACATATAAGATATGGAGAAATTGCTGCCGCCATAAAAGATGCTAAATTAAACGGAATAAAGGTTAAAGCATCCGATTATATGGTTATTGTAGAAGGAAAATTACAAAAAACATCAAAGAGTCTATTCAACGCAGCACAATTATTAGTATCAGAATTAGTTTCGGATGATAGTCAAGTAGTAACAATAATATATGGCCAAGATGTTTCGGAAAATGATGCGCTGGAATTACAAAATTATATTGAAACAACATACGACCTTGTTGTAGAAATTTATCAAGGTGACCAAGGAATTTATCCATATTTAATCTCAGTCGAATAG", "aa": "MSSTISSACNSECLEDDCSIESASRIVAQNMLMSARGNSGVILSQIFKGFSNGFASKKSANAFDLVNAFEMAAKSAYGAVLKPVEGTILTVIREVSEGLKNQVVVDTTIEEVFEIAVKLARKSCDGTPKLLKILKEVGVTDSGGEGLYAILQGMSAYFSGNKVEPIVAEESVEKFISDEEVYEGEFGYCTEFILELYKPKKFDKDYLVRKLEKIGNSMVVVNDDKILKVHIHTPKPGDVLNSVNSLGQFAKIKIDNMTLQANNSKKTAKKKQTEAPNNVELKNSAIVSCNTGQGIVTLMKENGVSYVVDGGQTNNPSIQDIVNAINAVNAKTVFVLPNNSNIILSAQQAGTIIRDKNVIIIPTRSQVQGLNVALNYSSENSDEENHELMSNSLKHIRYGEIAAAIKDAKLNGIKVKASDYMVIVEGKLQKTSKSLFNAAQLLVSELVSDDSQVVTIIYGQDVSENDALELQNYIETTYDLVVEIYQGDQGIYPYLISVE", "aa_hexdigest": "5bb31d35b2ee1bf9f12eae1f00b3cee6", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J8Q5", "query_cov": 1.0, "subject_cov": 0.9206642066420664, "identity": 0.998, "score": 931.0, "evalue": 0.0, "valid": true, "gene": "FakA", "product": "DhaL domain-containing protein", "uniref50_id": "UniRef50_N9TTI7", "cog_id": "COG1461", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_N9TTI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TTI7" ], "product": "DhaL domain-containing protein" }, "genes": [ "dhaL", "fakA" ], "id": "DFOKDJOJIG_1177", "locus": "GCA013367855_01176" }, { "type": "cds", "sequence": "contig_1", "start": 667434, "stop": 667913, "strand": "+", "frame": 3, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0008610", "GO:0043811", "SO:0001217", "UniRef:UniRef50_D1J8Q6", "UniRef:UniRef90_D1J8Q6" ], "nt": "ATGAAAAAAATAGTATTTGATTTATTGAATAATGATAATGGCGAAGAACAAGCGATTTTGGGTGCAAAAAGATTTTTACAAGAAAATTCAGATTATTTTTTAATTTTGGTTGGTCATGAACAACTTATCAAAAAACATTTATCAGATTACATTGATAGGATTGCCATAATTAATAATGAAACAATTGTACACATTGAATCTAGTCCAAGAGACGTATTGAGAACTCAAAATTCAATGCTAGAGGCTTTTAAACAACTCAACGAATCAAATGCAGATGGAATATTGAGTAGTGGAGATAGTGGCTCGTTAATAACATTATCATCATTGAAAGTTAAAAGGCTTCTTGGGATTGAAAGACCAGCATTCATGCCTATTCTGCCAACAACAACGAATAAGAAAATGTTATTAATAGATGCAGGGGCAAACATTGAAATCAAACCAGAATATTTAAGTCAATGGGCTATGATAGCAAGTGAATGA", "aa": "MKKIVFDLLNNDNGEEQAILGAKRFLQENSDYFLILVGHEQLIKKHLSDYIDRIAIINNETIVHIESSPRDVLRTQNSMLEAFKQLNESNADGILSSGDSGSLITLSSLKVKRLLGIERPAFMPILPTTTNKKMLLIDAGANIEIKPEYLSQWAMIASE", "aa_hexdigest": "2e5b95c3362473ab17203bd9dceff479", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "plsX" ], "truncated": "3-prime", "pseudogene": { "start": 667434, "stop": 668216, "observations": { "mutation": [ 667911, 668199 ], "stop": [ 667911, 668199 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8Q6", "query_cov": 1.0, "subject_cov": 0.4746268656716418, "identity": 1.0, "score": 306.0, "evalue": 1.41e-102, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MKKIVFDLLNNDNGEEQAILGAKRFLQENSDYFLILVGHEQLIKKHLSDYIDRIAIINNETIVHIESSPRDVLRTQNSMLEAFKQLNESNADGILSSGDSGSLITLSSLKVKRLLGIERPAFMPILPTTTNKKMLLIDAGANIEIKPEYLSQWAMIASEWYSILFGSKKPIVGQLNVGVEEYKGSEITKNAKNILKEVSKSANFVFNGYVEATNAINGDIDILVVDGYGGNVFLKTLEGTFIAFSKLLKNILLKNWKTKLAALILKRHLREFKQKFDYRNTGGAFIIGLEKIVVKAHGGSDELAFYNALNQIKIGIENNISTILNKKFKEADNEE" }, "paralog": false, "identity": 0.9923076923076923, "score": 496.0, "evalue": 6.02e-180, "description": "Internal stop codon at 667911, 668199. Nonsense mutation around 667911, 668199.", "psc": { "uniref90_id": "UniRef90_D1J8Q6", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_D1J8Q6", "cog_id": "COG0416", "cog_category": "I", "go_ids": [ "GO:0008610", "GO:0043811" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8Q6", "EC:2.3.1.274", "UniRef:UniRef50_D1J8Q6", "COG:COG0416", "COG:I", "GO:0008610", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8Q6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8Q6" ], "product": "Phosphate acyltransferase" } }, "id": "DFOKDJOJIG_1178", "locus": "GCA013367855_01177" }, { "type": "cds", "sequence": "contig_1", "start": 667923, "stop": 668201, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGAAGCAAAAAGCCAATAGTAGGACAATTAAACGTAGGTGTCGAAGAATACAAAGGTTCTGAAATAACAAAGAATGCTAAAAATATATTAAAAGAAGTTTCTAAATCTGCTAATTTTGTTTTTAATGGTTATGTTGAAGCAACAAATGCCATTAATGGAGATATAGATATATTAGTGGTTGATGGATATGGCGGAAATGTGTTTCTAAAAACATTAGAAGGAACCTTTATTGCTTTTTCAAAATTATTGAAAAATATTTTATTAAAAAATTGA", "aa": "MFGSKKPIVGQLNVGVEEYKGSEITKNAKNILKEVSKSANFVFNGYVEATNAINGDIDILVVDGYGGNVFLKTLEGTFIAFSKLLKNILLKN", "aa_hexdigest": "59450b628a35b04801e5ffb8ffc405e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9999.448399999992, "isoelectric_point": 8.892075920104983 }, "id": "DFOKDJOJIG_1179", "locus": "GCA013367855_01178" }, { "type": "cds", "sequence": "contig_1", "start": 668431, "stop": 668964, "strand": "+", "frame": 1, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "COG:COG0571", "COG:K", "EC:3.1.26.3", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396", "SO:0001217", "UniRef:UniRef50_D1J8Q7", "UniRef:UniRef90_D1J8Q7" ], "nt": "ATGAAGATTAATTTTGAAGAAAATTTACAAAATGCATTGCTAAAATTTGGTATAAATTTTCAAAAATTAGATGAACAAAAAAGACGTTATTTTTGTAATGCTTTTACTCATAGTTCATACAGCAACGAACATAAAAATTTAGAAAGTTATCAATATTTAGAATTTTTAGGTGACTCAGTTTTAGGTTTTGTGGTTTCTGATTTCATTTTTGATAATTATAAAAATTTCGGTGAGGGAAAGGCAACAAAATTAAAAAGTGAAATTGTTGACAAAAATGCGCTTGGATTCTTATCGAGATATTATGGTTTTCCAAACTGTTTGAGGACGGGCAAAAGTGCATTTGCAAACGGAAAAGGAATTAAATCAGATTCTGATCTAATTGAATCATTCATTGGTGCTTGTTATAAGGCCTGCGGTTTGGAATTTACTAAAGAAATTATTTATCATTTTATTGACAAAATATTAAAAAAATTTCACAATTCAATCAATGAACACCCTAAAACAAAATTGCAAGAACTTGTACAAAGCAATTGA", "aa": "MKINFEENLQNALLKFGINFQKLDEQKRRYFCNAFTHSSYSNEHKNLESYQYLEFLGDSVLGFVVSDFIFDNYKNFGEGKATKLKSEIVDKNALGFLSRYYGFPNCLRTGKSAFANGKGIKSDSDLIESFIGACYKACGLEFTKEIIYHFIDKILKKFHNSINEHPKTKLQELVQSN", "aa_hexdigest": "561ec69e9aef7c9b37f874edcc50fd5b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rnc" ], "truncated": "3-prime", "pseudogene": { "start": 668431, "stop": 669126, "observations": { "mutation": [ 668962 ], "stop": [ 668962 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8Q7", "query_cov": 1.0, "subject_cov": 0.7662337662337663, "identity": 0.9940000000000001, "score": 358.0, "evalue": 1.21e-124, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MKNNFEENLQNALLKFGINFQKLDEQKRRYFCNAFTHSSYSNEHKNLESYQYLEFLGDSVLGFVVSDFIFDNYKNFGEGKATKLKSEIVDKNALGFLSRYYGFPNCLRTGKSAFANGKGIKSDSDLIESFIGACYKACGLEFTKEIIYHFIDKILKKFHNSINEHPKTKLQELVQSNWGCRPRYEDQALNNGKFESKVFINNNLYGEGIGQSKKESQTKAANDALKKMAKI" }, "paralog": false, "identity": 0.987012987012987, "score": 464.0, "evalue": 1.66e-168, "description": "Internal stop codon at 668962. Nonsense mutation around 668962.", "psc": { "uniref90_id": "UniRef90_D1J8Q7", "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_D1J8Q7", "cog_id": "COG0571", "cog_category": "K", "go_ids": [ "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8Q7", "EC:3.1.26.3", "UniRef:UniRef50_D1J8Q7", "COG:COG0571", "COG:K", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8Q7" ], "product": "Ribonuclease 3" } }, "id": "DFOKDJOJIG_1180", "locus": "GCA013367855_01179" }, { "type": "cds", "sequence": "contig_1", "start": 669148, "stop": 672084, "strand": "+", "frame": 1, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0005524", "GO:0007059", "GO:0030261", "RefSeq:WP_175972350.1", "SO:0001217", "UniParc:UPI00158DBC59", "UniRef:UniRef100_UPI00158DBC59", "UniRef:UniRef50_A0A448ZX91", "UniRef:UniRef90_D1J8Q8" ], "nt": "ATGAAGTTAATTCAAGTTGAAGCACACGGTTTTAAATCATTTGCAGATAAAGTAACCCTAAAATTTGACGGTGGAATTGTTGCAATAATAGGACCAAATGGGTCAGGAAAATCAAACATAAATGATGCAATCAGATGGGTGTTAGGTGAAACTAGTTCCAAAGTGTTGCGTGGAGATACAATGGAAGACGTTATCTTTTCAGGTTCTAAGACTGAAAAAGAAATGGATCGAGCAGAAGTAATTTTAACATTTGACAATAAGGATAGATCTTGTGATATCCCATATGATGTTTTTACTATTTCTAGAGTATTAACTAGAGGAAATGGTTCAAATGAATATTATATTAATGGTGAATTAGCAAGGCAAAGAGACATTAAAGAAATTGCAATGCAAAGTGGTATTTCAAAATCCAGTTTAGCAATCATAGGTCAAGGAACTATTTCCAACATTGCCGAATCAACTCCTGAAAAACGTAGAGAAATATTTGAAGATGCTGCTGGAACTTCAATGTACAAAAGCAAGAAGATTGAAGCACAAAGAAAATTAGAAAAAACCCAAGAAGCATTGGAACAAATTTCAATATTAATTCAAGAACAAGAAAGACAATTAAAGCCATTGCAACGTCAAGCTGAAAAAGCAAAAATTTATAAAACAAAAGCAGAACAATTAAAAGAAGTTGAAGTAGCATTATTGGTTCATGATTTCATGGATTATTCAAATAAATTAGAAAAATTAAATGTTGAGGGGCAAGATTACAATCTAATTAAAGAAGAATTAGAAACTAAAATAAGAGTATACAACGAAGCAAGCGAACAAAAAAGCAAGTTTGTTTTAGAATTAGAAAATAACATAAAAAAGATAAGCGAAAGATTAAGCGAAATTAGCGAAGAAATTACGACCCTAGAAATAAGAAACGCAAAAGAAGCAAAACACCGCGAAATGCTTTTAAGTGGCGAACTAACTATATCCCCAAAAGAAAAATTAGCAGCAATGAAAGAAGAATTGAATGCATTAAATACTAATATTTTAGGGTATAAGACATTCTTAGAAACCAAAAAGAAGGAATCTGAACAATTATCTCAATCAGCTTCAATTAGATCAACAAAAATAAATGATATAAAACGAGAATTATCCTTTAAAAAAGAAGAACTTAATAAGATAAAATCAAAATTAGATGTTATTGAAGAAATAAAATCTAAAAAGACAAATTTGGCTAAAGGAACACATAATATTGTTGAAAACTCTCATTTATTTAGAGGATATAAAGCATTGGTTAGTGAATTAATTGAAGTTAATGAAAAATATGCCTTAGCTATTGAAACAATTCTAAGCTCCGCTATGCAACATATAGTTGTTGACAAACCAGAAACAGCAGTTGAAGCAATCAATTTCTTAAAATCAAATAATGGAGGTAGAGCAACTTTTATTCCTTTGTCATCAATTAAACCAAAATTCGTTCCTGAACAACATATTGTTGTTGCTCAAACCCAAAAAGGTTTTCTAGGAATTGCTTCGGAACTTGTTTCAACAGAGGAACAATATAATGTTTTAAAGAAATTCTTACTAGGCAATGTTTTAGTTGCAGATAACATTGAAAATGCAAATAAATTATCTAAATTGCTAGAAACTCGTTATATGGTCGTAACGCTAGATGGCGACATCATAAGAGTTGGTGGAGTTCTTTCAGGAGGACAAGCAAGTCAAAACGTTTCCACATTAGCAGCTGATGAACAAGTTAAAAAATTACAAGACCTTATTCCAGTAATTAATGCAAAAATTTCACAATTATCAAATGAGTTGTTTGAATTAGAAAAAGACCAAAATAAAGAAACATCATTATTAACAAATATTTCTCTTGAAAGAGCAAAGATTGACAACTTATATAGAACAACATTGGATAAGTTTGATTCATTGCGTGAACAATACCGTAGTGCTTCGCAAGAAGAATATGAGGCAGAAAACAACAAAAAAATTGATATAGTAGCAGCAATTCAACAATTAATGCTTGAAAGAAGTGATTTATCAGCTAAAAAATCATCTCAAGAAGATATTTATCATAATCTAAAACAAGAATTACAAAATCTTACCAATCAACGCTTTCAAGTTAGTGAAGAATTAAATAATTTGATTAAAGATAATGCAAACAAGATAGCAGAAAAAGCAAAAGCAGAAACAATTATTCAAAATGCAAAACAAAGATTGTCAGAACAATATGGAATGCTATTTGAAGTGGCAAAACAATTTTACAATCCAGATATAGATTTTGAAGTAGCAAGAAAAACAGTTGCTGATTTGAAATTGGATATAAGAGAGTTAGGACATGTAAACCTTGATTCAATTAACCAATTAGAAGAACTTGAAACAAGATATAAAGAAGTAAAATCTCAAGAACAAGAAATAATTTCAGCAAAAACCACCATTGAAGATGCAATATCAGAAATGGATAAAATTATTATTGAAAGAATGACACAAACAGTTACATTAGTCAATGCTGAATTCAAGCATGTGTTTGCAAAAATGTTTGGCGGAGGTATGGCTGAAATAAGATATACTGACCCTGAAAACATTCTTGAAACAGGAATTGACGTCATTGCTCAACCTCCAGGAAAATCTATTAAGAATTTAAAATTATTTAGTGGGGGTGAAAAGGCACTTATAGCTATTTCATTACTATTTTCAATATTAAAAGCTAAGCCATTGCCATTGTGTATCTTAGACGAAGTTGAAGCAGCACTAGATGAAGCAAACGTAATAAGATTTGCTGAATATTTGCACACTCTAAAAAAAGATACCCAATTTATAGTGATTACTCACCGTCAAGGAACAATGGAAAGAGTAAACAAACTTTATGGCGCAACGATGCAAAAAAGAGGGGTTACAACATTTTTTGCAGTAAATCTTTCAGACGCAAAAAAGGCAATTGAAAACAACAATTAA", "aa": "MKLIQVEAHGFKSFADKVTLKFDGGIVAIIGPNGSGKSNINDAIRWVLGETSSKVLRGDTMEDVIFSGSKTEKEMDRAEVILTFDNKDRSCDIPYDVFTISRVLTRGNGSNEYYINGELARQRDIKEIAMQSGISKSSLAIIGQGTISNIAESTPEKRREIFEDAAGTSMYKSKKIEAQRKLEKTQEALEQISILIQEQERQLKPLQRQAEKAKIYKTKAEQLKEVEVALLVHDFMDYSNKLEKLNVEGQDYNLIKEELETKIRVYNEASEQKSKFVLELENNIKKISERLSEISEEITTLEIRNAKEAKHREMLLSGELTISPKEKLAAMKEELNALNTNILGYKTFLETKKKESEQLSQSASIRSTKINDIKRELSFKKEELNKIKSKLDVIEEIKSKKTNLAKGTHNIVENSHLFRGYKALVSELIEVNEKYALAIETILSSAMQHIVVDKPETAVEAINFLKSNNGGRATFIPLSSIKPKFVPEQHIVVAQTQKGFLGIASELVSTEEQYNVLKKFLLGNVLVADNIENANKLSKLLETRYMVVTLDGDIIRVGGVLSGGQASQNVSTLAADEQVKKLQDLIPVINAKISQLSNELFELEKDQNKETSLLTNISLERAKIDNLYRTTLDKFDSLREQYRSASQEEYEAENNKKIDIVAAIQQLMLERSDLSAKKSSQEDIYHNLKQELQNLTNQRFQVSEELNNLIKDNANKIAEKAKAETIIQNAKQRLSEQYGMLFEVAKQFYNPDIDFEVARKTVADLKLDIRELGHVNLDSINQLEELETRYKEVKSQEQEIISAKTTIEDAISEMDKIIIERMTQTVTLVNAEFKHVFAKMFGGGMAEIRYTDPENILETGIDVIAQPPGKSIKNLKLFSGGEKALIAISLLFSILKAKPLPLCILDEVEAALDEANVIRFAEYLHTLKKDTQFIVITHRQGTMERVNKLYGATMQKRGVTTFFAVNLSDAKKAIENNN", "aa_hexdigest": "aecfc1856c779792df7e9f00b530acc8", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00158DBC59", "ncbi_nrp_id": "WP_175972350.1", "uniref100_id": "UniRef100_UPI00158DBC59", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158DBC59", "RefSeq:WP_175972350.1", "UniRef:UniRef100_UPI00158DBC59" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158DBC59", "uniref90_id": "UniRef90_D1J8Q8" }, "psc": { "uniref90_id": "UniRef90_D1J8Q8", "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_A0A448ZX91", "cog_id": "COG1196", "cog_category": "D", "go_ids": [ "GO:0005524", "GO:0007059", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZX91", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZX91" ], "product": "Chromosome partition protein Smc" }, "genes": [ "smc" ], "id": "DFOKDJOJIG_1181", "locus": "GCA013367855_01180" }, { "type": "cds", "sequence": "contig_1", "start": 672343, "stop": 672462, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGGGTTAGTTTTTTATTTAGATAAAGAAATATTAAAAAAAGAATACTCAGATAGTAATCATAAAGAAGCTTATGAAAAAGTAAGAGATATATTGAGGCACTACGGATTTCATTGA", "aa": "MYGLVFYLDKEILKKEYSDSNHKEAYEKVRDILRHYGFH", "aa_hexdigest": "3e2077c335083dcd249c2a105b696001", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4836.4392, "isoelectric_point": 6.817859840393066 }, "id": "DFOKDJOJIG_1182", "locus": "GCA013367855_01181" }, { "type": "cds", "sequence": "contig_1", "start": 672834, "stop": 673511, "strand": "-", "frame": 2, "gene": null, "product": "Leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R0", "UniRef:UniRef90_D1J8R0" ], "nt": "TTGCTAGATTTATCAAAAACCAATATAACTTCAATACCCGCAGGATCATTTTCTTATCTAGTTATGCAAAATTTGGCAGACGATGGTGCTGGCTTACCAACTGAATTATCAACATTAGTTACAACTAATTCTTCAAATAACAACAAAAAAATAAATATTAGAAAAATTATTTTGCCAAAAACCTTAAAATCAATTGGCGCAGGTGCTTTTTATGGATTAAGTAACCTAGAAGAAGTAGATTTTTCTCAATGCAACGACTTATCTCAAATAGATGCTGAAGCTTTTTCAAATACAAATATTAAAAAATTACAATTGCCTGCATCTTTAAATAAGTTAGGACAAAAAGCCTTTGCAAACAGTGGCATTACATCTGTAAATTTAGAAGATTTACCTTATTTAAAAACTCTTACACAAGGTGTTTTTGCTTCAAATAACCTTACAAGCATAAACTTAAAAAACATTGAATTAGTTGAAAGCGGAGCCTTGGCAAATAATCAAATAAAAACAATAGAAATTCCTTCAACAATGAAATCATTTAGTCCTGAATCATTTGACTTTATTGGTGCGGGGGAATCAGCTGCTAAAACTCTTATTTCTAAAATTGAAAACAATGATATTCAATCGTTTTTAGAAAAAGCTTTGAAGGAAAACCCAAATCATCTATATAAATTAAACTAA", "aa": "MLDLSKTNITSIPAGSFSYLVMQNLADDGAGLPTELSTLVTTNSSNNNKKINIRKIILPKTLKSIGAGAFYGLSNLEEVDFSQCNDLSQIDAEAFSNTNIKKLQLPASLNKLGQKAFANSGITSVNLEDLPYLKTLTQGVFASNNLTSINLKNIELVESGALANNQIKTIEIPSTMKSFSPESFDFIGAGESAAKTLISKIENNDIQSFLEKALKENPNHLYKLN", "aa_hexdigest": "b99d6ad1dedeb3c5a9b09bde99425da3", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 672834, "stop": 673697, "observations": { "mutation": [ 673529 ], "stop": [ 673529 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8R0", "query_cov": 1.0, "subject_cov": 0.7839721254355401, "identity": 0.9420000000000001, "score": 378.0, "evalue": 1.46e-130, "gene_start": 1, "gene_end": 225, "reference_start": 63, "reference_end": 287, "reference_sequence": "MKKNKKFLFLGFGLVAPFIAGLTAISCGGEKFNAFALNHVQGPLKDPKNSTNHNQTWFAESGLLDLSKTNITSIPAGAFSYLVMQNLADEGAGLPTELSTLVTTNSSNNNKKINIRKIILPKTLKSIGAGAFYGLSNLEEVDFSQCNDLSQIDAEAFSNTNIKKLQLPASLNKLGQKAFANSGITSVNLEDLPYLKTLTQGVFASNKLTNINLKNIELVESGALADNQIKTIEIPSTMKSFSPESFDFIGAGESAAKTLISKIENHEIESFLKDALKKNPNHLYKLS" }, "paralog": false, "identity": 0.9512195121951219, "score": 504.0, "evalue": 5.84e-183, "description": "Internal stop codon at 673529. Nonsense mutation around 673529.", "psc": { "uniref90_id": "UniRef90_D1J8R0", "product": "Leucine-rich repeat domain-containing protein", "uniref50_id": "UniRef50_D1J8R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8R0", "UniRef:UniRef50_D1J8R0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R0" ], "product": "Leucine-rich repeat domain-containing protein" } }, "id": "DFOKDJOJIG_1183", "locus": "GCA013367855_01182" }, { "type": "cds", "sequence": "contig_1", "start": 673527, "stop": 673697, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGAACAAAAAATTTTTATTTTTAGGCTTTGGTTTAGTTGCTCCATTTATTGCTGGATTAACAGCAATATCTTGTGGAGGAGAAAAGTTCAATGCTTTTGCTCTAAATCATGTGCAAGGCCCACTAAAAGACCCTAAGAACTCATCCAATCACAATCAAACTTGA", "aa": "MKKNKKFLFLGFGLVAPFIAGLTAISCGGEKFNAFALNHVQGPLKDPKNSSNHNQT", "aa_hexdigest": "b8eb387af183d42d9da3b0f1b50a8ec6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6047.9625000000015, "isoelectric_point": 9.903455924987792 }, "id": "DFOKDJOJIG_1184", "locus": "GCA013367855_01183" }, { "type": "cds", "sequence": "contig_1", "start": 673791, "stop": 675761, "strand": "+", "frame": 3, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "EC:3.1.25.-", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887", "RefSeq:WP_175972352.1", "SO:0001217", "UniParc:UPI00158CF832", "UniRef:UniRef100_UPI00158CF832", "UniRef:UniRef50_B3PLX9", "UniRef:UniRef90_D1J8R1" ], "nt": "ATGGAAAAGTTTAAATTAGTTTCAAATTATAAGCCAGCCGGCGACCAACCTCAGGCAATAAATGAGTTGGTTCGCGGTGTTTTAGAAGGTAAAGAGCATCAAGTTTTATTAGGCGTAACTGGTTCAGGAAAAACATTTACTATGGCAAATGTCATTAATCAGTTAAATAGGCCTACATTGGTTATAAGCCATAACAAAACATTAGCAAGTCAACTTTATAGCGAATTAAGGGAATTATTTCCTGAAAATAGAGTTGAATATTTTGTTTCGTATTTTGATTTTTATAAGCCAGAAGCGTACATTCCTAAGTCTGATTTATATATCGAAAAAACAAGTAGGAACAATAAAGATTTAGAAGCAATGAGGATGTCTGCTCTTAATGCCCTAAGCATTCGAAGAGATACTATTGTTGTTGCATCTGTTGCTGCAATATATGCCGAATTTAACCCCAAAGAATATATTCAAAGTTTTATGCCTATTGAAAGCCATATGAAAATTAAGTTGCGTGATCTTTTAACCAAGTTGGTTAATTTAGGTTATGAAAAGCATAATGGTGATTTATCTTCTGGACAATTTTTCAATAAGGGGGAAGTTATTGAAATTTGTCCCGGATATACTTCAGAAAACAATATTCGTATTGAGATGTTTGATGATTATATTGAAAGAATTTCATTAATTAATCCTTTAACAAAGTCAATAGTTGAAGTGCGTAAGTCGTTGACTATTTTTCCTGCAACTACCTATAACGTAGAAAAAGGTTATATTGAAAATATTTGCAATAGCATTGAAAGTGAATTAAAAGAAAGGGTTGCCTATTTTAAATCACAAAATCAACTTTTGGAAGCCCAAAGGATTCACGATAAAACTTTAAATGATATTGATTCATTAAAGGAATTTGGTTATGTTAAGGGTATTGAAAATTACGCTCGATATGTTGATAAACGCGAACCCGGACAAAAACCATACACAATATTCGATTATTTACCAAAGGATGCAGTAATTTTTGTCGATGAAAGTCACCTTATGATTCCTCAAATAATAGGAATGTACGAAGGAGATCGTTCTAGAAAAGAAACACTAATTAAATATGGTTTTAGATTGCCTTCTGCCTTGGATAATAGACCTTTAAAATTTAATGAATATGAAGAATATAAATTTCCAAGAATTTATATGTCAGCAACTCCTGGCGACTATGAAATAAATAAGGCAAATGGTGAACTAGTTACTCAATACATTAGACCAACAGGCCTTTTAGATCCCATTATTGAAATACATTCAAAAGAAAATCAAATTCAAGACATTCATGATTTATTAAAATTACAAATAGAAAAAAAAGAAAGAACTTTAATTTTGACTACAACCAAAAAGAATGCCGAAGAGCTTTCTTTATACCTTTCTGAATTTAAAATTAAAAGTGCATATATTCATGATGGATTTAAAACCTTTGAAAGAAATGAAATCTTAAAAGGTTTAAGAATGGGAAAATATGATGTTGTAATTGGAATTAATTTATTGCGTGAAGGTATTGATTTGCCAGAAGTCAGTTTGATTTGTGTATTAAATGCAGATTCTATGGGGCTAATGCGTGATGCTAGAAGTTTGATTCAAATAGTTGGTCGAGCCTCTAGAAATGATCATGGAAAAGTAATATTTTATGCCGATAATATTACAACCAGTATGCAAAAAGCAATTGTTGACAATGAACATAAAAGAACATTACAAATTAAATATAATAAAGAACACAACATTACCCCTAAAAGTATTATTAAACCAATACCCGCACCTATTCAAGGCAGTATTTTATTAGAAGATAATAAGGCAATAAAAGAAGCCAATAGAACTATTCATAATAAAAAATATATTGATAATATGATTAAACATATGAAAGAATTAGCAAGGGCAAAAAAATACGAAGAAGCTATTGAAATAAGAGATTATTTGATTGAATTAGGTGTCGAATTTGACGATTAA", "aa": "MEKFKLVSNYKPAGDQPQAINELVRGVLEGKEHQVLLGVTGSGKTFTMANVINQLNRPTLVISHNKTLASQLYSELRELFPENRVEYFVSYFDFYKPEAYIPKSDLYIEKTSRNNKDLEAMRMSALNALSIRRDTIVVASVAAIYAEFNPKEYIQSFMPIESHMKIKLRDLLTKLVNLGYEKHNGDLSSGQFFNKGEVIEICPGYTSENNIRIEMFDDYIERISLINPLTKSIVEVRKSLTIFPATTYNVEKGYIENICNSIESELKERVAYFKSQNQLLEAQRIHDKTLNDIDSLKEFGYVKGIENYARYVDKREPGQKPYTIFDYLPKDAVIFVDESHLMIPQIIGMYEGDRSRKETLIKYGFRLPSALDNRPLKFNEYEEYKFPRIYMSATPGDYEINKANGELVTQYIRPTGLLDPIIEIHSKENQIQDIHDLLKLQIEKKERTLILTTTKKNAEELSLYLSEFKIKSAYIHDGFKTFERNEILKGLRMGKYDVVIGINLLREGIDLPEVSLICVLNADSMGLMRDARSLIQIVGRASRNDHGKVIFYADNITTSMQKAIVDNEHKRTLQIKYNKEHNITPKSIIKPIPAPIQGSILLEDNKAIKEANRTIHNKKYIDNMIKHMKELARAKKYEEAIEIRDYLIELGVEFDD", "aa_hexdigest": "fd63a4ec6cc63504cf4a9bdf25bea6df", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158CF832", "ncbi_nrp_id": "WP_175972352.1", "uniref100_id": "UniRef100_UPI00158CF832", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158CF832", "RefSeq:WP_175972352.1", "UniRef:UniRef100_UPI00158CF832" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158CF832", "uniref90_id": "UniRef90_D1J8R1" }, "psc": { "uniref90_id": "UniRef90_D1J8R1", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "ec_ids": [ "3.1.25.-" ], "uniref50_id": "UniRef50_B3PLX9", "cog_id": "COG0556", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLX9" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "DFOKDJOJIG_1185", "locus": "GCA013367855_01184" }, { "type": "cds", "sequence": "contig_1", "start": 676005, "stop": 676400, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J842", "UniRef:UniRef90_D1J842" ], "nt": "ATGGCAAAAATTAATGGTTCAATTATTTCAGAATATGAAATTAAAAAAATTAAAAATTTAGATTTTATTTCCAAAAATTTGCAAAGAAAAAATAGTTATTTGCAAGAAAAAACAATGTTATCAATTCCTACAATTTGTAGATATAAAAAAATTATTAAAGATAATATGCAAGGTAGCAAAATTGAAGTAAATATTAGACATAAAAACTTTAATAATCAAAATGCAAAAATAATAGAAAACGATTTTGCAAAAGACTTATTTTTGAAATATCAACTTGAAAATCAAAAAATAAATTCAAATGTTACTAAAAAATTTATTACTGTCAAAGATTTTTATGAAGACCTTAGTGAAGAAATAAGGTCAAAAATTTCGCTATCATATTTATATAATGCTTGA", "aa": "MAKINGSIISEYEIKKIKNLDFISKNLQRKNSYLQEKTMLSIPTICRYKKIIKDNMQGSKIEVNIRHKNFNNQNAKIIENDFAKDLFLKYQLENQKINSNVTKKFITVKDFYEDLSEEIRSKISLSYLYNA", "aa_hexdigest": "f3611ef0448c14cb40a5e100e8619658", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 676005, "stop": 676512, "observations": { "deletion": [ 676464, 676467 ], "mutation": [ 676398 ], "stop": [ 676398, 676473 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J842", "query_cov": 1.0, "subject_cov": 0.7751479289940828, "identity": 0.9690000000000001, "score": 245.0, "evalue": 1.96e-81, "gene_start": 1, "gene_end": 131, "reference_start": 1, "reference_end": 131, "reference_sequence": "MAKINGSIISKYEIKKIKNLDFISKNLQRKNSYLQEKTMLSIPTICRYKKIIKDNMQGSKIKVNIRHKNFNNQNAKIIENDFAKDLFLKYQIENQKINSNVTKKFITVKDFYEDLSEEIKSKISLSYLYNAWLKHGFCSPYATHKIKRIAKNIFQIWKLMTAKLAIVKN" }, "paralog": false, "identity": 0.9644970414201184, "score": 310.0, "evalue": 1.11e-109, "description": "Internal stop codon at 676398, 676473. Frameshift due to deletion around 676464, 676467. Nonsense mutation around 676398.", "psc": { "uniref90_id": "UniRef90_D1J842", "uniref50_id": "UniRef50_D1J842", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J842", "UniRef:UniRef50_D1J842" ] }, "pscc": { "uniref50_id": "UniRef50_D1J842", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J842" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15521.795199999988, "isoelectric_point": 9.560999870300293 }, "id": "DFOKDJOJIG_1186", "locus": "GCA013367855_01185" }, { "type": "cds", "sequence": "contig_1", "start": 676470, "stop": 676703, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015884C7E", "UniRef:UniRef90_UPI0015884C7E" ], "nt": "TTGAAAATTAATGACAGCAAAATTAGCAATAGTGAAAAATTAATTTATAAGGCGAATATTTTATTGATTGAAAACACTAAAAAATTGTCAATATATTCAAATAAAAATCAAAATTATAAATTTGGTGAAATTATTGAAGTAGATGCTTGCCAAGATATATTTTTCAAAACCAATGAAAAAATTCATATTTATCATGCAATTGATGCTAAAAGCGGTGCATTAGTTGCCTTGTGA", "aa": "MKINDSKISNSEKLIYKANILLIENTKKLSIYSNKNQNYKFGEIIEVDACQDIFFKTNEKIHIYHAIDAKSGALVAL", "aa_hexdigest": "8990d6cab888fcedfe2fdac6facec40b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 676470, "stop": 676876, "observations": { "deletion": [ 676842 ], "mutation": [ 676701 ], "stop": [ 676701 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0015884C7E", "query_cov": 1.0, "subject_cov": 0.5703703703703704, "identity": 0.9740000000000001, "score": 146.0, "evalue": 1e-43, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MKINDSKISNSEKLIYKANILLIENTKKLSIYSNKNQNYKFGEIIEVDACQDRFFVTNEKIHIYHAIDAKSGALVALWAEKEKTNKGYQKLFELNQKYLDQQNKTTIEIIKNQTKIYNELIEINQKINDFIWQKI" }, "paralog": false, "identity": 0.9555555555555556, "score": 249.0, "evalue": 1.02e-86, "description": "Internal stop codon at 676701. Frameshift due to deletion around 676842. Nonsense mutation around 676701.", "psc": { "uniref90_id": "UniRef90_UPI0015884C7E", "uniref50_id": "UniRef50_UPI0015884C7E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0015884C7E", "UniRef:UniRef50_UPI0015884C7E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0015884C7E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015884C7E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8806.081499999997, "isoelectric_point": 8.687711143493651 }, "id": "DFOKDJOJIG_1187", "locus": "GCA013367855_01186" }, { "type": "cds", "sequence": "contig_1", "start": 676967, "stop": 678178, "strand": "+", "frame": 2, "gene": null, "product": "DUF262 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D746A", "UniRef:UniRef90_UPI0030CE4285" ], "nt": "ATGGCTAATGAGTATTATAATATGTGTGCCTTAGATGCACTCAAATTTGTGAAAAATAGAAATATGTTATTACCAGATATTCAAAGAGAATATGTTTGGGATACAACTGATATAGAAAAATTATTTGAATCTATAGTTGATGATTATCCAGTGGGTTCTTGTATATTTTGGAAAACAAATAAAAAAGTACTTAATGATGAAAAACCAAATCTTTATTATTTTATTTCTAAATATAAGCAAGGCGTAACTAAAAATGAAAAAGCACCAGAATTTGTATCTGATGAAACAGATTATTATATAGTATTAGATGGACAACAAAGAATTACATCTCTTAATATTGCATTGTTTGGATCGTTTACATCTAAGAAAAAAGGTAAAGGATATAGTGTAAAAAATTCTAAATCATGGGTAGAACGAGAACTATATTATAATTTATCTTTTTACGACAGCAATGAATTGGATGATGAAAATCCCATTAAAAGATTTGTATTTTTGTCTGAAGAAGAAGCAAAGAATGGTAATTATTATAAAGTTAAGCAAATAGTTAAAAGTGATAACTTAACTGATTATGTTGATACAATATCAAATTTTAACAAAAATGTAAAAACGGATTTAATGTGTTTGTATAGAAGAATTATGGAAGCGCCTGCCACTAGTGTAATTCATTGTTATTGCATTGCAGAGAATACATATGATGAAGCACTAGATATTTTTGTTAGAGTAAATTCTACTGGAAGAAAACTATCAAAGTCCGATTTATTATTTTCTACATTAATTGATGGTTGGAAAGAAGGGAAGGAAAATATAGACACCTTATTGTCTGACTTAAATTCAGCCGGAGATGGATTTTCTTTTACAAAAGATTATTTAATGCGATTGGCTTTGGTATTAGTTGATGCAAATACTAATTTAAAAATTAATTCGTTAACTTCTAAAACAATAAATGAAATCAGGGATAACTGGCAAAAAATAAAGAAAGCGCTAGAAAAATTATCTGAATTATTAGTTTCAATTGGCATGTGTGACGAAAATATGACCTCATATAATGCAACCATTCCACTGGCATATTATTTTTACAAAGGTGGTAAAATTGATACAGCAGAATCAAAAAAAGAAGCAAGAAAGTTCCTAGCCGTTTCAATGTCAAAAGGATTGTTTGGTGTAGCAAGTAATGATTCTTTGAATCAAAGCAGAACCCTCTTAAGTCAGTAA", "aa": "MANEYYNMCALDALKFVKNRNMLLPDIQREYVWDTTDIEKLFESIVDDYPVGSCIFWKTNKKVLNDEKPNLYYFISKYKQGVTKNEKAPEFVSDETDYYIVLDGQQRITSLNIALFGSFTSKKKGKGYSVKNSKSWVERELYYNLSFYDSNELDDENPIKRFVFLSEEEAKNGNYYKVKQIVKSDNLTDYVDTISNFNKNVKTDLMCLYRRIMEAPATSVIHCYCIAENTYDEALDIFVRVNSTGRKLSKSDLLFSTLIDGWKEGKENIDTLLSDLNSAGDGFSFTKDYLMRLALVLVDANTNLKINSLTSKTINEIRDNWQKIKKALEKLSELLVSIGMCDENMTSYNATIPLAYYFYKGGKIDTAESKKEARKFLAVSMSKGLFGVASNDSLNQSRTLLSQ", "aa_hexdigest": "615281087bdffddcacf263ff877edddf", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0030CE4285", "query_cov": 0.9478908188585607, "subject_cov": 1.0, "identity": 1.0, "score": 758.0, "evalue": 3.69e-276, "valid": true, "product": "DUF262 domain-containing protein", "uniref50_id": "UniRef50_UPI00215D746A" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D746A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D746A" ], "product": "DUF262 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_1188", "locus": "GCA013367855_01187" }, { "type": "cds", "sequence": "contig_1", "start": 678274, "stop": 678561, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002411044F", "UniRef:UniRef90_UPI002411044F" ], "nt": "TTGACGAAGTTTGAAAAAGGAAAAAACACATTTATAATTCTTTCTTTGCTTTATCCTAATTTAAAGTTAAGTCAAGTTGAGTTCCATCAGGATCACTGTCACCCTTATACTTTATTTGATGATAAAAACATTAAAAAATTAGGATTGACAGAAGATAAAATAAAAGAATGGCAGTTTAAAAGAAATTTAATTCCTAATTTGCAGTTTTTAGTAGGTAAGGAAAATGAGTCAAAAAATAAAACGCCTTTATCAGATTGGGTTAAAAAAGGAAATACCTTTGAATATTGA", "aa": "MTKFEKGKNTFIILSLLYPNLKLSQVEFHQDHCHPYTLFDDKNIKKLGLTEDKIKEWQFKRNLIPNLQFLVGKENESKNKTPLSDWVKKGNTFEY", "aa_hexdigest": "ed8aa7da86e3335dd5616799193c51d8", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 678319, "stop": 678675, "observations": { "mutation": [ 678559 ], "stop": [ 678559 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002411044F", "query_cov": 0.8421052631578947, "subject_cov": 0.6779661016949152, "identity": 0.988, "score": 171.0, "evalue": 1.55e-53, "gene_start": 16, "gene_end": 95, "reference_start": 1, "reference_end": 80, "reference_sequence": "MLYPNLKLSQVEFHQDHCHPYTLFDDKNIKKLGLTEDKIKEWQFKRNLIPNLQFLVGKENESKNKTPLSDWVKKGNTFEYWPENVSLELSNFDEFFNKRKSLIKNKLLDIFGLEANQK" }, "paralog": false, "identity": 0.9830508474576272, "score": 243.0, "evalue": 2.92e-84, "description": "Internal stop codon at 678559. Nonsense mutation around 678559.", "psc": { "uniref90_id": "UniRef90_UPI002411044F", "uniref50_id": "UniRef50_UPI002411044F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002411044F", "UniRef:UniRef50_UPI002411044F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002411044F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002411044F" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11331.941699999988, "isoelectric_point": 9.187276649475098 }, "id": "DFOKDJOJIG_1189", "locus": "GCA013367855_01188" }, { "type": "cds", "sequence": "contig_1", "start": 678914, "stop": 679888, "strand": "+", "frame": 2, "gene": null, "product": "Abi-like protein", "db_xrefs": [ "RefSeq:WP_175972354.1", "SO:0001217", "UniParc:UPI001588B563", "UniRef:UniRef100_UPI001588B563", "UniRef:UniRef50_R7K8H8", "UniRef:UniRef90_UPI0026F27AE7" ], "nt": "ATGTCATCAAACAAAGTAGAAAGATTCGACTACTCTAAATACGAATCAGTGTCAATAAACGACCATGATACTTTAGGACAATATCAATTCTGGGGTATTCATCCTAAAGGCGGAAGATTAAGAATCGATACAAAACGTTATAAAAAAATGTGTGAATGGATAGGAATCACTGACTTTATGCCAGATGGTTTGTTTGTGCCAAGAAGCACTGCTTATTTTACACCAGTAAAAAAGGAAAGATACGACTATAAAGTAAATCTATTTAATGACTTATTAGATGGATTAATTCATGATTGGGAGTATGAATACAAACCATTATTTAAATCCATAAAGACACCAGATCAAGTGGCGAGCGATAGTCGTCTCGGAATGCTTGCTTTCACAGGTTGTTCAGATGACTATGATGAAATTGACATAGAGGCAAAGATGAATGGCTTTAGAAGAATAGAAAAATATAATCAAATTATTAATTCTTTACATTGTCAATTTATATTGAAGATTGGTGCGGAAGTAGACCGATTTACCTTAAGGGTAATGACCGAACTTGGATATAAAGGTACTGACTACGATTTTAAATCATTTTGTAAATTTTCTGATGGATTATTAAGAAGTAAAAATGGTATGCCATTAAAAGATTTAAAGAAGTTTGAAGCGTACAACATGCTTCATAAAATATGCAATTTTCTAAAACATAATTCAAAAGAAGCATACAACACAATGAAACATTTGTATCCAGATAATGTTTGTTCCAAAGAAAAAGGAACCGCTAGTACGGATTTTGAAAATGGTATGTTTGCAGGTGATTGGATTATCATTAAAGAAGGTTATATTGATAAGACATTAAAACAATTAAAAATATTCTTCACTGATTACTGCAAAGTCTACTTGAAAGAAGACGTAGATAATGCGTATTGGAATTATGATGATTATTTTAAAGATGCAGTTTCTCAGATGCGTTATCCATACGATTATTAG", "aa": "MSSNKVERFDYSKYESVSINDHDTLGQYQFWGIHPKGGRLRIDTKRYKKMCEWIGITDFMPDGLFVPRSTAYFTPVKKERYDYKVNLFNDLLDGLIHDWEYEYKPLFKSIKTPDQVASDSRLGMLAFTGCSDDYDEIDIEAKMNGFRRIEKYNQIINSLHCQFILKIGAEVDRFTLRVMTELGYKGTDYDFKSFCKFSDGLLRSKNGMPLKDLKKFEAYNMLHKICNFLKHNSKEAYNTMKHLYPDNVCSKEKGTASTDFENGMFAGDWIIIKEGYIDKTLKQLKIFFTDYCKVYLKEDVDNAYWNYDDYFKDAVSQMRYPYDY", "aa_hexdigest": "bd41a0ddd673ff51ceb677755340ca99", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI001588B563", "ncbi_nrp_id": "WP_175972354.1", "uniref100_id": "UniRef100_UPI001588B563", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588B563", "RefSeq:WP_175972354.1", "UniRef:UniRef100_UPI001588B563" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588B563", "uniref90_id": "UniRef90_UPI0026F27AE7" }, "psc": { "uniref90_id": "UniRef90_UPI0026F27AE7", "uniref50_id": "UniRef50_R7K8H8" }, "pscc": { "uniref50_id": "UniRef50_R7K8H8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R7K8H8" ], "product": "Abi-like protein" }, "genes": [], "id": "DFOKDJOJIG_1190", "locus": "GCA013367855_01189" }, { "type": "cds", "sequence": "contig_1", "start": 679989, "stop": 680468, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CD69E8A", "UniRef:UniRef90_UPI000CD69E8A" ], "nt": "ATGAACACCGATATTAAGGTGCCGAAGGTCAAAGGGATGTTACTAGGTAGAACACCTTTAGGCCATAAAACTCTTAGAGATGAGACTATCATCGCTAGAATCATTGAAGAAGACGAACCCGCTACTGTGATTGACAATGGCTGGGACACAGTTATTAGGTTGCATTACAAATTAAAGTCAAAAAGGAATAAAGCATATAATACTAAAGCACTAGTGTGGATTGAATTTCTAAAGTCATTATTGTGGTTAGATGAAATACCAGCTGTATGGTTTCCAAAAGAAAAAGGTTTTATTAAGTATGGTGATGTGGTTTTTGTGATGTTAAAATCAGGAGAGTTTTTCTACTCAAGAAGCATCGCACATAGATTATCGCCACTAGATCTAAAATGGAAATTGCATAACCAAAAGAAAAACACCGAGTATGAACTAGGTGACATTTTTTGTTTATCAAGTGTTAGTTCCATACGATTTATACGATGA", "aa": "MNTDIKVPKVKGMLLGRTPLGHKTLRDETIIARIIEEDEPATVIDNGWDTVIRLHYKLKSKRNKAYNTKALVWIEFLKSLLWLDEIPAVWFPKEKGFIKYGDVVFVMLKSGEFFYSRSIAHRLSPLDLKWKLHNQKKNTEYELGDIFCLSSVSSIRFIR", "aa_hexdigest": "0baf336979e04a0bda7168c257a4defb", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI000CD69E8A", "query_cov": 1.0, "subject_cov": 0.9352941176470588, "identity": 1.0, "score": 326.0, "evalue": 4.32e-113, "valid": true, "uniref50_id": "UniRef50_UPI000CD69E8A" }, "pscc": { "uniref50_id": "UniRef50_UPI000CD69E8A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CD69E8A" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18597.57980000001, "isoelectric_point": 9.565577125549318 }, "id": "DFOKDJOJIG_1191", "locus": "GCA013367855_01190" }, { "type": "cds", "sequence": "contig_1", "start": 680628, "stop": 680786, "strand": "+", "frame": 3, "gene": null, "product": "helix-turn-helix domain-containing protein", "db_xrefs": [ "RefSeq:WP_155392205.1", "SO:0001217", "UniParc:UPI000A452C3A", "UniRef:UniRef100_UPI000A452C3A", "UniRef:UniRef50_UPI000A452C3A", "UniRef:UniRef90_UPI000A452C3A" ], "nt": "ATGATTATCGAAGAAAAAAATATGATTATTAAATTAAGTGTTGATGGATTGGACTATAGGCAAATAGCCAGTCAATTAGATTTAAATAAATCTACAGTATCATCTTTTTTCAAAGGGAAAGTAAGAAAAAAATACGAGAGGTTCCTCGACCTTTTGTAA", "aa": "MIIEEKNMIIKLSVDGLDYRQIASQLDLNKSTVSSFFKGKVRKKYERFLDLL", "aa_hexdigest": "1ad98c08e9c4fe7a90963ec525fee006", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000A452C3A", "ncbi_nrp_id": "WP_155392205.1", "uniref100_id": "UniRef100_UPI000A452C3A", "db_xrefs": [ "SO:0001217", "UniParc:UPI000A452C3A", "RefSeq:WP_155392205.1", "UniRef:UniRef100_UPI000A452C3A" ] }, "ips": { "uniref100_id": "UniRef100_UPI000A452C3A", "uniref90_id": "UniRef90_UPI000A452C3A" }, "psc": { "uniref90_id": "UniRef90_UPI000A452C3A", "product": "helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_UPI000A452C3A" }, "pscc": { "uniref50_id": "UniRef50_UPI000A452C3A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A452C3A" ], "product": "helix-turn-helix domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_1192", "locus": "GCA013367855_01191" }, { "type": "cds", "sequence": "contig_1", "start": 681046, "stop": 681186, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019393E90", "UniRef:UniRef90_UPI0019393E90" ], "nt": "TTGGAAGCTAATATAGATCAAGATATATATCAAGAAACCTTGCCATTAAACAAAAGCGATAAATCATTAATTGTTAATGATTTAGAACGAAGGATTAATTCAATTCTTCCTAATTTAAATATTTTAAAAGAGACTGTTTAG", "aa": "MEANIDQDIYQETLPLNKSDKSLIVNDLERRINSILPNLNILKETV", "aa_hexdigest": "7b309d6d967de4227ab905b3406637e1", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 681046, "stop": 681368, "observations": { "deletion": [ 681326 ], "stop": [ 681245 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019393E90", "query_cov": 1.0, "subject_cov": 0.42990654205607476, "identity": 0.935, "score": 84.0, "evalue": 5.43e-20, "gene_start": 1, "gene_end": 46, "reference_start": 62, "reference_end": 107, "reference_sequence": "MIIFENTENKTYYCIRLQTARKNTTKYNLQIDNSSYQTNEYWKNHKGVAITKDVFIIGKEVLEANIDQDIYQETLPLNKSDKSLIVNDLERGINSIPPNLNILKETV" }, "paralog": false, "identity": 0.9252336448598131, "score": 189.0, "evalue": 6.54e-64, "description": "Internal stop codon at 681245. Frameshift due to deletion around 681326.", "psc": { "uniref90_id": "UniRef90_UPI0019393E90", "uniref50_id": "UniRef50_UPI0019393E90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019393E90", "UniRef:UniRef50_UPI0019393E90" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0019393E90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019393E90" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5325.011199999998, "isoelectric_point": 4.507070732116699 }, "id": "DFOKDJOJIG_1193", "locus": "GCA013367855_01192" }, { "type": "cds", "sequence": "contig_1", "start": 682119, "stop": 682655, "strand": "-", "frame": 2, "gene": null, "product": "Site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "EC:2.1.1.72", "KEGG:K06223", "SO:0001217", "UniRef:UniRef50_N9U9M3", "UniRef:UniRef90_A0A6A8Q0M8" ], "nt": "TTGTCAGATGAAGAAAAGGCTGCCAGAATTATTTATTTGAATAAAGCATGTTTTAATGGTTTATATCGTGTAAATAAAAATGGTTTTTTTAATGTTCCATCAGGAAAGAAAGAAAAAGTCAATTGCTATAATAAAGAAAACTATAATTCTATTTTTGAATATTTTTCTAATGTAGATTACAAAATAACAAATTTAAATTTTGAAGATGCAATAAAAGATGCAAAAGCTGGCGACTTGGTTTATTTTGACCCTCCTTATGATACCTTAGAAAATAAAAATTCATTTACTTCTTATTCAAATGAAACCTTTGGAAAAAACGAGCAAATTAAACTTGCTAATGTTTTTAAGGAACTGTCAAAAAAGGGCGTTTATGTAATATTAAGTAATCACAAAACTGATTTTGTTTGCAATTTATACAGGGATTTTAATATTAATATAATTAAAGCAAGAAGAAACATTAATAGAAATGGTAAAAATCGAGGCGATGTCGAAGAGGTAATCATTACTAATTTTGATAAAGAGGATCTATGCAAATAA", "aa": "MSDEEKAARIIYLNKACFNGLYRVNKNGFFNVPSGKKEKVNCYNKENYNSIFEYFSNVDYKITNLNFEDAIKDAKAGDLVYFDPPYDTLENKNSFTSYSNETFGKNEQIKLANVFKELSKKGVYVILSNHKTDFVCNLYRDFNINIIKARRNINRNGKNRGDVEEVIITNFDKEDLCK", "aa_hexdigest": "be6cb92bf59efba8133b74dbb5064f7b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00241138B0", "query_cov": 0.9606741573033708, "subject_cov": 0.8636363636363636, "identity": 0.515, "score": 185.0, "evalue": 1.71e-56, "valid": false, "product": "Dam family site-specific DNA-(adenine-N6)-methyltransferase", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7C6Y5M6", "kegg_orthology_id": "K06223" }, "genes": [], "truncated": "both", "pseudogene": { "start": 682101, "stop": 682954, "observations": { "deletion": [ 682822 ], "mutation": [ 682945 ], "stop": [ 682120, 682945 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8Q0M8", "query_cov": 1.0, "subject_cov": 0.6180555555555556, "identity": 0.966, "score": 346.0, "evalue": 9.74e-119, "gene_start": 1, "gene_end": 178, "reference_start": 105, "reference_end": 282, "reference_sequence": "MIKPFVKWAGGKTQILEKLLNFIPKKFNNYYEPFIGGGSLLFSLLPKNFCINDINSELVLAYKCFAKKESFDKLKSLLDLHTSKHSNEYYYQIRNLDRQPEYKNLSDEEKAARIIYLNKACFNGLYRVNKNGFFNVPSGKKEKVNCYNKENYNSIFEYFSNVDCKITNLNFEDAIKDAKAGDLVYFDPPYDTLENKHSFTSYSNETFGKNEQIKLANVFKKLSKKGVYVILSNHKTDFVCNLYRDFNINIIKARRNINRNGKNRGDVEEVIITNFNKEVLCKQDLKNF" }, "paralog": false, "identity": 0.954225352112676, "score": 551.0, "evalue": 5.46e-202, "description": "Internal stop codon at 682120, 682945. Frameshift due to deletion around 682822. Nonsense mutation around 682945.", "psc": { "uniref90_id": "UniRef90_A0A6A8Q0M8", "product": "Site-specific DNA-methyltransferase (adenine-specific)", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_N9U9M3", "kegg_orthology_id": "K06223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8Q0M8", "EC:2.1.1.72", "UniRef:UniRef50_N9U9M3", "KEGG:K06223" ] }, "pscc": { "uniref50_id": "UniRef50_N9U9M3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U9M3" ], "product": "Site-specific DNA-methyltransferase (adenine-specific)" } }, "id": "DFOKDJOJIG_1194", "locus": "GCA013367855_01193" }, { "type": "cds", "sequence": "contig_1", "start": 683141, "stop": 683308, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGCAAACGTTGAATATGACGAAAATAAATCATTGTATGAACAAGTTGTGCAATTGATAGAAGGAATAAAAAATACTAACTCGCCTAAAGCAAAATTATTAATTAATGAAATTATTGATTTATACAATTCTTATTTTTTAACCTTTGGAAGTAACGGATATTGA", "aa": "MLANVEYDENKSLYEQVVQLIEGIKNTNSPKAKLLINEIIDLYNSYFLTFGSNGY", "aa_hexdigest": "bbd5d4a825c89f2742549f76079c9cc4", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6319.061599999999, "isoelectric_point": 4.4943956375122065 }, "id": "DFOKDJOJIG_1195", "locus": "GCA013367855_01194" }, { "type": "cds", "sequence": "contig_1", "start": 683765, "stop": 683896, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGAAATAAGCAAGGAAAATACATTTATAAACTAAACCAACAAGTAAAGGCGTTATTAAAAGCATATATTGATTTAATGGGTTTTGGCCAACCTATTAGTTATATGGGTTCTGATATTAGCAGTTGA", "aa": "MFRNKQGKYIYKLNQQVKALLKAYIDLMGFGQPISYMGSDISS", "aa_hexdigest": "96a83be845c6bf6d953683381d428873", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4946.7649, "isoelectric_point": 9.698317527770996 }, "id": "DFOKDJOJIG_1196", "locus": "GCA013367855_01195" }, { "type": "cds", "sequence": "contig_1", "start": 683945, "stop": 684403, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACGATGAATATTTAGTCAATAAAGAATTATTTAATCCTAAAAACAAAGAAATACCAGTTTATTCAAAATTTAGTCAATATGACTTATTCAAGTTAGCTAACCTCGCTTTAACAAACAAAGAAGTTGAAAAATACAATATTTTAAACAATAAAAACTTTTATTTTAATAATTCAACAAACAACACAGTTTATTATGCAAATTCTACTAATATTGATCAAATAAAATACTTGTTTTCATTTGAGGAACTTTTTGCAAGAGAACTTGTAAAACTTTCATATTCAGCACATGAAAATTTTCAAATGTTTGACGGTCATTTAACTTTGGATGATTCTTTGAATTTCTTATATTATAAATTAAAAAATGATGATATTTTTCTATCATCAATTGGTGATGACACATTGAAAATGATTGCCAACTATTATAATAAATATGTAATATATTCAGCCAATTGA", "aa": "MQNDEYLVNKELFNPKNKEIPVYSKFSQYDLFKLANLALTNKEVEKYNILNNKNFYFNNSTNNTVYYANSTNIDQIKYLFSFEELFARELVKLSYSAHENFQMFDGHLTLDDSLNFLYYKLKNDDIFLSSIGDDTLKMIANYYNKYVIYSAN", "aa_hexdigest": "5f049ff731051e5c7e1c3bf5367e4449", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18093.072999999997, "isoelectric_point": 5.147134590148924 }, "id": "DFOKDJOJIG_1197", "locus": "GCA013367855_01196" }, { "type": "cds", "sequence": "contig_1", "start": 684632, "stop": 685126, "strand": "-", "frame": 3, "gene": null, "product": "MYPU_1760 family metalloprotease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D", "UniRef:UniRef90_UPI001E491E9D" ], "nt": "TTGGCTTTTATATTAACGGCAGGTATTATTGGCGTCGTAATATATTACATTTTTAATTATGTTAATTATTCAAATGGCAATGGTGGGAGTTTATTTGATGGCCAAAAAAATAAAAATTATTCAATGCCAAATGAAGAAAGATATCTACAAAGTTTCAATGCAAAAACAATTACCAAAAACAATGTTCAATTAAAGAACAAGAATTATCAATATGGAAATCTAAGTATTTTTGAATATCCTTTTGCTTTAACAAAGAATAATGAACCTATTTATTTTTTGGGTGAAGAAGGAAGAAAATTATTGAATTTAGAGTTTTTAAAAAGAGGATTTTATGGTCCCGAAATTAATGGTTTAAATCATGTTTATATAAATAAAAAAATACCTAATGTTTCGAATATTAAAGATGTAAATGGTGTTTATTTGCCACAAGCTTTTGACATTTTTATTCAAATAAATCATTTTATCTCTGGGGGAATAAAACAAGTCCAATCATGA", "aa": "MAFILTAGIIGVVIYYIFNYVNYSNGNGGSLFDGQKNKNYSMPNEERYLQSFNAKTITKNNVQLKNKNYQYGNLSIFEYPFALTKNNEPIYFLGEEGRKLLNLEFLKRGFYGPEINGLNHVYINKKIPNVSNIKDVNGVYLPQAFDIFIQINHFISGGIKQVQS", "aa_hexdigest": "bebc9f95ee69908618904ce1786ae235", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 684329, "stop": 685148, "observations": { "insertion": [ 685106 ], "stop": [ 684634 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E491E9D", "query_cov": 1.0, "subject_cov": 0.4039408866995074, "identity": 0.945, "score": 318.0, "evalue": 3.32e-106, "gene_start": 1, "gene_end": 164, "reference_start": 8, "reference_end": 171, "reference_sequence": "MGNIYYNLGFYINGGIIGVVIYYIFNYVNYSNGNGGGLFDGQKNKNYSMPNEERYLQSFNAKTIVKNNIQLKNKNYQYGNLSIFEYPFALTKNNEPIYFLGEEGRKLLNLEFLKRGFYGPEINGLNHVYINKKIPNVSNIKDVNGVYLPQAFDIFIQINHFISGGIKQVQSWSLQKRVELVFNTLFHEYAHHIDNTYNRSIRENDEFANNDLSYKLATPELNKIINKFNGNNNKFLTEFRKTLNYDGSMQNDEYLVNKELFNPKNKEIPVYSKFSQYDLFKLANLALTNKEVEKYNILNNKNFYFNNSTNNTVYYANSTNIDQLKYLFSFEELFARELVKLSYSAHENFQMFDGHLTLDDSLNFLYYKLKNDDIFLSSIGDDTLKMIANYYNKYVIYSPIEYLMMN" }, "paralog": false, "identity": 0.9852941176470589, "score": 549.0, "evalue": 9.55e-200, "description": "Internal stop codon at 684634. Frameshift due to insertion around 685106.", "psc": { "uniref90_id": "UniRef90_UPI001E491E9D", "product": "MYPU_1760 family metalloprotease", "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E491E9D", "UniRef:UniRef50_UPI001E491E9D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D" ], "product": "MYPU_1760 family metalloprotease" } }, "id": "DFOKDJOJIG_1198", "locus": "GCA013367855_01197" }, { "type": "cds", "sequence": "contig_1", "start": 685259, "stop": 685711, "strand": "+", "frame": 2, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "db_xrefs": [ "COG:COG0742", "COG:J", "EC:2.1.1.171", "GO:0031167", "GO:0052913", "KEGG:K08316", "SO:0001217", "UniRef:UniRef50_A0A077L643", "UniRef:UniRef90_A0A454CAI7" ], "nt": "ATGTTGAGAATAATAGCAGGAAAATATAGATCCAGAATTCTAAAGCAACCTTCCAAGTCAATAACAAGACCAACAATTGATAGAGCAAGGGAGGCAATATTTTCTTCAATTCAGTTTAACATTGAAAATAAGAAAGTTTTAGATTTATTTTCGGGATCGGGAGCATTTTGTTTAGAAGCTCTATCAAGGGGCGCAAAATTCGCGGTATGTGTTGATAAAAATGCTGAGGCTATTAAAATAATTAAAGAAAATCAAAATAGTTTAAAAGAACCAAATTTAAAAATTTTTAATATTGATGCATTGAATTTTTTAAATAATAATGTTGAAGTATTTGATTATATTTATTTAGATCCCCCATTTGTTGAAAAAAAATTACTAGTTGAATGTTTAAATAAAATTATTGATAAAAAATGTATTGATAAAAGTGGGACAATGATAATTGAAACAGATTGA", "aa": "MLRIIAGKYRSRILKQPSKSITRPTIDRAREAIFSSIQFNIENKKVLDLFSGSGAFCLEALSRGAKFAVCVDKNAEAIKIIKENQNSLKEPNLKIFNIDALNFLNNNVEVFDYIYLDPPFVEKKLLVECLNKIIDKKCIDKSGTMIIETD", "aa_hexdigest": "b71f3fdbd296b995342c242285c41994", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAI7", "query_cov": 1.0, "subject_cov": 0.8152173913043478, "identity": 0.993, "score": 292.0, "evalue": 1.81e-99, "valid": true, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "ec_ids": [ "2.1.1.171" ], "uniref50_id": "UniRef50_A0A077L643", "cog_id": "COG0742", "cog_category": "J", "kegg_orthology_id": "K08316", "go_ids": [ "GO:0031167", "GO:0052913" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L643", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L643" ], "product": "N6-adenine-specific methylase" }, "genes": [ "rsmD" ], "id": "DFOKDJOJIG_1199", "locus": "GCA013367855_01198" }, { "type": "cds", "sequence": "contig_1", "start": 685865, "stop": 686488, "strand": "+", "frame": 2, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "COG:COG0194", "COG:F", "EC:2.7.4.8", "GO:0004385", "GO:0006163", "RefSeq:WP_012855758.1", "SO:0001217", "UniParc:UPI0001BC2503", "UniRef:UniRef100_D1J8S4", "UniRef:UniRef50_Q98PN5", "UniRef:UniRef90_D1J8S4" ], "nt": "ATGAGTAAAAAATTAATAATATTTACAGGACCAAGTGGCGTTGGAAAAGGAACAATTGAAAAGCCCTTATTTGAAGATAAAAGTCTAAAGCTAAAATTATCAGTATCAATGACAACGAGATCACCACGTGAAGGAGAAATTGATGGTGTACATTATTACTTTGTGTCAAAAGATACTTTTAACTCTTGCTTAGCAGAAAATAAGCTAATAGAATATTCAATGCACTTTGATAATTATTATGGAACATTATATTCAGAAATTGAACGTATTAGTGAACAAGGAAAAATTCCATTTCTTGAAATTGAAACTAATGGTGCTAAACAAATTTTAGAATATTATAAAAAACGTAACAGATTGGATGAAATTTGTTCAATATTTTTAATGCCTCCTTCATATGGAGAATTAAGAAAAAGAATTGAAGGTCGCAATACCGAAACTGAAGATCTAATTATTAAAAGAATGAAGAAAGCAAAAGAAGAAATAGGTTATTCTACAATATTTCATCATGTGATAGTTAATGATGATTTAAATGTAGCACTTGAAGAAATCAAAGAAATAATCAGATCAGAATTTTCAGATATTATAAACAAAAAAGAAGTTGCAAGTGAAGGTAGAAAGGCATAA", "aa": "MSKKLIIFTGPSGVGKGTIEKPLFEDKSLKLKLSVSMTTRSPREGEIDGVHYYFVSKDTFNSCLAENKLIEYSMHFDNYYGTLYSEIERISEQGKIPFLEIETNGAKQILEYYKKRNRLDEICSIFLMPPSYGELRKRIEGRNTETEDLIIKRMKKAKEEIGYSTIFHHVIVNDDLNVALEEIKEIIRSEFSDIINKKEVASEGRKA", "aa_hexdigest": "458f5bbf2abd014048df359e386016bd", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2503", "ncbi_nrp_id": "WP_012855758.1", "uniref100_id": "UniRef100_D1J8S4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2503", "RefSeq:WP_012855758.1", "UniRef:UniRef100_D1J8S4" ] }, "ips": { "uniref100_id": "UniRef100_D1J8S4", "uniref90_id": "UniRef90_D1J8S4" }, "psc": { "uniref90_id": "UniRef90_D1J8S4", "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_Q98PN5", "cog_id": "COG0194", "cog_category": "F", "go_ids": [ "GO:0004385", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN5" ], "product": "Guanylate kinase" }, "genes": [ "gmk" ], "id": "DFOKDJOJIG_1200", "locus": "GCA013367855_01199" }, { "type": "cds", "sequence": "contig_1", "start": 686582, "stop": 686725, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGCTTTGTGTGATGGTATGGGCGGACATGTTGGCGGTAGCCAATGTTCAACATTATCAACTAATAGTATTAAACAATATTTCCTAGACACATTTCCTCAAAAGTTGGAATGTAGTGATAAAAAAAACGTTAGCAAATGA", "aa": "MAALCDGMGGHVGGSQCSTLSTNSIKQYFLDTFPQKLECSDKKNVSK", "aa_hexdigest": "897f954bd8244134008f6051081b9bdd", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5026.679600000001, "isoelectric_point": 7.717677879333496 }, "id": "DFOKDJOJIG_1201", "locus": "GCA013367855_01200" }, { "type": "cds", "sequence": "contig_1", "start": 686810, "stop": 687247, "strand": "+", "frame": 2, "gene": "pTC1", "product": "Protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_D1J8S5", "UniRef:UniRef90_D1J8S5" ], "nt": "ATGGTTGTTGCCCTCATTTTTAATGCAAATGGCTTAGCTTATGTCTTTAATATTGGCGATTCACGCTTGTATGCATACAATGGATTACTTTACCAAATCACAGAAGATCAAAATTATTTATATCAATTGATGAGAGAATTTAATTTAACATACGAAGAAGCGGCATTAGATCCCAATTCTTACAAACTTATAAGTTGTTTAGGCCCCAATAAAAAAACCAATTGTCAGTCATTTTTTATATCACAAAAATCAGCAGTTAAATATTATTTATTAACATCCGATGGATTACACGATTATGTTTCTAAACCAATAATAGAAACTGTTTTGCAAACAAATAAGAGTTTAAAAGATAAGTTAAACCTTCTAATAAAATATGCCAAAAAAAATCTTTCAAAAGACAATATAACCGGAATTCTTGTGGGGTTGAAAAATGAATAA", "aa": "MVVALIFNANGLAYVFNIGDSRLYAYNGLLYQITEDQNYLYQLMREFNLTYEEAALDPNSYKLISCLGPNKKTNCQSFFISQKSAVKYYLLTSDGLHDYVSKPIIETVLQTNKSLKDKLNLLIKYAKKNLSKDNITGILVGLKNE", "aa_hexdigest": "065e93331d98713cea8319b7fb60eda0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "pTC1" ], "truncated": "5-prime", "pseudogene": { "start": 686510, "stop": 687247, "observations": { "mutation": [ 686576, 686723 ], "stop": [ 686576, 686723 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S5", "query_cov": 1.0, "subject_cov": 0.5776892430278885, "identity": 1.0, "score": 284.0, "evalue": 2.29e-95, "gene_start": 1, "gene_end": 145, "reference_start": 107, "reference_end": 251, "reference_sequence": "MVDFCIASDQGKFRKENQDRAIVVNGEYWTLAALCDGMGGHVGGSQCSTLSTNSIKQYFLDTFPQKLECSDKKNVSKWFNNAISFIKEALVNYAKEYTEFEDMGTTMVVALIFNANGLAYVFNIGDSRLYAYNGLLYQITEDQNYLYQLMREFNLTYEEAALDPNSYKLISCLGPNKKTNCQSFFISQKSAVKYYLLTSDGLHDYVSKPIIETVLQTNKSLKDKLNLLIKYAKKNLSKDNITGILVGLKNE" }, "paralog": false, "identity": 0.9918367346938776, "score": 484.0, "evalue": 8.65e-177, "description": "Internal stop codon at 686576, 686723. Nonsense mutation around 686576, 686723.", "psc": { "uniref90_id": "UniRef90_D1J8S5", "gene": "PTC1", "product": "Protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_D1J8S5", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S5", "EC:3.1.3.16", "UniRef:UniRef50_D1J8S5", "COG:COG0631", "COG:T", "KEGG:K20074" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S5" ], "product": "Protein phosphatase" } }, "id": "DFOKDJOJIG_1202", "locus": "GCA013367855_01201" }, { "type": "cds", "sequence": "contig_1", "start": 687240, "stop": 687491, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00069.31", "PFAM:PF07714.23" ], "nt": "ATGAATAAGATAAAAGAATTACCCGGCATGTTTGTACTTAATAAGTATTTTACTGATTTTGAATTAATTGGGACTGGAGGATTTGGCGAAGTTTATTCTGCTACATATAAAAAAAATAATAAAAGAGTTGCAATTAAAATACTAAATTGTATCGATGATAGCCGCTGGACAGAGCAGAGAACGAGATTCATTAACGAAATAAATATATTAAAGGAAATTCATAGTTCTAACGTAGTAAAGATATTAGGCTGA", "aa": "MNKIKELPGMFVLNKYFTDFELIGTGGFGEVYSATYKKNNKRVAIKILNCIDDSRWTEQRTRFINEINILKEIHSSNVVKILG", "aa_hexdigest": "3129f318d6e5da1aabbfaa3dd80b3c2b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00069.31", "name": "Pkinase", "length": 65, "aa_cov": 0.7831325301204819, "hmm_cov": 0.9692307692307692, "evalue": 1.8253665728071004e-13, "score": 46.0883674621582, "start": 18, "stop": 82 }, { "id": "PF07714.23", "name": "PK_Tyr_Ser-Thr", "length": 67, "aa_cov": 0.7590361445783133, "hmm_cov": 0.9552238805970149, "evalue": 1.017715732668728e-11, "score": 40.26829147338867, "start": 21, "stop": 83 } ], "seq_stats": { "molecular_weight": 9613.061799999996, "isoelectric_point": 9.37326793670654 }, "id": "DFOKDJOJIG_1203", "locus": "GCA013367855_01202" }, { "type": "cds", "sequence": "contig_1", "start": 687522, "stop": 688256, "strand": "+", "frame": 3, "gene": "sPS1", "product": "mitogen-activated protein kinase kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "SO:0001217", "UniRef:UniRef50_D1J8S6", "UniRef:UniRef90_D1J8S6" ], "nt": "ATGGAGTTAATTAAAGGCCAAGGTTTAAAAACAATAATTGCAAAAAACAAAAAAATCGATCCTGATACTGCAATTATGTATGCAAAGCAAATATGTCAAGGTTTGATTGAAATCCATGCTGCCAAAATAGTTCATAGAGATTTGAAACCAAGCAATATATTAATTGATGAAGAAACAAATAGCCTAAAATTAATTGATTTTGGCATCTCAATAGGCGAATCAACTCAACGTGTAACTAGAAATTATAAAGCTGTTGGTTCTGTTCAATATATGGCACCTGAATTATTGCGTGGAATAGCTGATCCTTCTCCGAGAAGTGATATATATGCATTTGGTATAATTTTGTTTGAAATGCTTGAAGGCAAGGTTCCTTTTAATAATCATGATGATATTAACCAAATTATGATGATGCACATCAATAATGAATTGCCTAGAATGAGTGATGTCAATGTTATTATTCCACAAGCATTAGAAAATATTGTAATTCGTTGCACTGCAAAATCTGCAGAGCAAAGATATACTGATTGTGTTGAACTAATGCATGATTTAGAGACATGTTTAGATTCAAAAAGAGTAAATGAAAAAAGATTGGAATTAAATCCAAGTAAATCAAAAATGAAAGCCAAAAGAATCTTTAATAGTAAATTATTTAATATTATTTTTTTCACAACAGGGGGACTTTTAGTAGCCTTTATTGTGATATTTGTGGCTTTATTTTTAAAGGGGATATTGTAG", "aa": "MELIKGQGLKTIIAKNKKIDPDTAIMYAKQICQGLIEIHAAKIVHRDLKPSNILIDEETNSLKLIDFGISIGESTQRVTRNYKAVGSVQYMAPELLRGIADPSPRSDIYAFGIILFEMLEGKVPFNNHDDINQIMMMHINNELPRMSDVNVIIPQALENIVIRCTAKSAEQRYTDCVELMHDLETCLDSKRVNEKRLELNPSKSKMKAKRIFNSKLFNIIFFTTGGLLVAFIVIFVALFLKGIL", "aa_hexdigest": "1bdf0cdc84ceb4df97f1179ccf0791a5", "start_type": "ATG", "rbs_motif": null, "genes": [ "sPS1" ], "truncated": "5-prime", "pseudogene": { "start": 687240, "stop": 688256, "observations": { "mutation": [ 687489 ], "stop": [ 687489 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S6", "query_cov": 1.0, "subject_cov": 0.7218934911242604, "identity": 0.992, "score": 466.0, "evalue": 3.69e-164, "gene_start": 1, "gene_end": 244, "reference_start": 95, "reference_end": 338, "reference_sequence": "MNKIKELPGMFVLNKYFTDFELIGTGGFGEVYSATYKKDNKRVAIKILNCIDDSRWTEQRTRFINEINILKEIHSSNVVKILGWHYKGNEVFYVMELIKGQGLKTIIAKNKKIDPDTAIMYAKQICQGLIEIHAAKIVHRDLKPSNILIDEETNSLKLIDFGISIGESTQRVTRNYKAVGSVQYMAPELLRGIADPSPRSDIYAFGIILFEMLEGKVPFNNHDDINQIMMMHINNELPRMSDVNVIIPQALENIVIRCTAKSAEQRYTDCVELMHDLETCLDSKRVNEKRLELNPSKSKMKAKRIFNSKLFNIIFFTTGGFLVAFIVIFVVLFLKGIL" }, "paralog": false, "identity": 0.9881656804733728, "score": 661.0, "evalue": 8.95e-244, "description": "Internal stop codon at 687489. Nonsense mutation around 687489.", "psc": { "uniref90_id": "UniRef90_D1J8S6", "gene": "SPS1", "product": "mitogen-activated protein kinase kinase", "uniref50_id": "UniRef50_D1J8S6", "cog_id": "COG0515", "cog_category": "T", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S6", "UniRef:UniRef50_D1J8S6", "COG:COG0515", "COG:T" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S6" ], "product": "mitogen-activated protein kinase kinase" } }, "id": "DFOKDJOJIG_1204", "locus": "GCA013367855_01203" }, { "type": "cds", "sequence": "contig_1", "start": 688257, "stop": 689108, "strand": "+", "frame": 3, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.1.3.100", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022", "KEGG:K06949", "RefSeq:WP_012855761.1", "SO:0001217", "UniParc:UPI0001BC2506", "UniRef:UniRef100_D1J8S7", "UniRef:UniRef50_D1J8S7", "UniRef:UniRef90_D1J8S7" ], "nt": "ATGATAGAAGGTAAAATAGTTAAATCAGTTGCTGGTTTTTATGATATAAAAAGTTTTGAAGATAAACAAATTTATCGTGTAAGAGGAGGAGGAAAACTTCGCTTGCTTGATATAGTGCCAATTGTGGGTGACAACGTTTTGTTTAAGAAAGACGAATTTATCTATCAAATTTTAGGTCGCAAGAATTTTTTTATTAGACCTAAAATTGCAAATGTTGATCAAGCAATTGTTGTGATGTCGTTGGTTGAACCTGAATTCAGTTCATTGCTCGTTGATAAATTTTTGATTATTATTGAAAGTAAGAATGTTAAGCCTATTATCGTATTAACAAAAAAAGATCTAACTTCTGATTCAAAAATAGCTTTTTATAAAGATCAAGGCTATGAAGTTTTTGAAATTAATTACGAAACAGGGCAAGGATTTGAAGGCTTAGGAGAAATATTTAAACATAAGACAAGCTTCTTTGTTGGTCAAACAGGAGTTGGAAAAACTACCTTGATAAATTATTTGGCAAAAACAAATTTTGAAACACAAGCAATTTCTAAAGCCTTAAACCGTGGAAAGCATACAACAAGAGAAGTTAGTTTGATTGATTTTAACGGTGGTGAAATAATTGATACACCAGGTTTTTCTTCAATAGAATTTGATTTAACAATTGAAGAGCTACCGATTGCTTTTAAATCGTTTAGAGAGGCTGCAAGCAAATGCAAATTTAGAACAAGTTGTAAACATTATTTAGAATCCGTTGAGGATTGCGAAGTTAAAAAATTAGTTTTACAAGGCAAAATTAAACAAGAAAGATATGATAATTACATTAGTTTTTTAAAAAGACTAACAAATCCAATAAAATAG", "aa": "MIEGKIVKSVAGFYDIKSFEDKQIYRVRGGGKLRLLDIVPIVGDNVLFKKDEFIYQILGRKNFFIRPKIANVDQAIVVMSLVEPEFSSLLVDKFLIIIESKNVKPIIVLTKKDLTSDSKIAFYKDQGYEVFEINYETGQGFEGLGEIFKHKTSFFVGQTGVGKTTLINYLAKTNFETQAISKALNRGKHTTREVSLIDFNGGEIIDTPGFSSIEFDLTIEELPIAFKSFREAASKCKFRTSCKHYLESVEDCEVKKLVLQGKIKQERYDNYISFLKRLTNPIK", "aa_hexdigest": "f44e87b5c254bf98cf5c0ce92220b61b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2506", "ncbi_nrp_id": "WP_012855761.1", "uniref100_id": "UniRef100_D1J8S7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2506", "RefSeq:WP_012855761.1", "UniRef:UniRef100_D1J8S7" ] }, "ips": { "uniref100_id": "UniRef100_D1J8S7", "uniref90_id": "UniRef90_D1J8S7" }, "psc": { "uniref90_id": "UniRef90_D1J8S7", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.1.3.100", "3.6.1.-" ], "uniref50_id": "UniRef50_D1J8S7", "cog_id": "COG1162", "cog_category": "J", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S7" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" }, "genes": [ "rsgA" ], "id": "DFOKDJOJIG_1205", "locus": "GCA013367855_01204" }, { "type": "cds", "sequence": "contig_1", "start": 689222, "stop": 689764, "strand": "+", "frame": 2, "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "SO:0001217", "UniRef:UniRef50_A0A448ZXM9", "UniRef:UniRef90_A0A454CAB1" ], "nt": "ATGGATGGAATTTTTGTAAACAATACTGCTTTAATATTTCAAGAAATCAAGCAAGTTAAAGAAAATTGTAAGAAACATACAATGGATATTCACTTGATGGTTAAAGATGTTTTCAAATATTATGAAATGTACAAGAATATTGGAGATATATTGACATTTCATTATGAAGCATTTGAAAATAGTGATCTTGAAAAGATGATTAAACAATGCCAAAAAGATAACGTTAAACTTGGCTTAGCAATAAAACCTAATACACAAGTTGAAGAAATAAGACAATATTTAAAACATTGTTCTTTGGTTTTAATAATGAGTGTTGAACCAGGCTTTGGTGGCCAAAAATTTATTGAAAATTCATTTAATAAAATAAAGGAATTAGCAGACATTCGTAAGGCTTTAGATTTAAACTTTATTATTGAAGTGGATGGTGGAGTTAAAGACCAAAATATTGCCAAATGTTTTGAATGTGGCGTTAATTTAGCAGTAGTTGGTTCTTATTTAGTAAAAAACTTTTCAAAAGAAACAATTGACCAACTTTTAAAATAA", "aa": "MDGIFVNNTALIFQEIKQVKENCKKHTMDIHLMVKDVFKYYEMYKNIGDILTFHYEAFENSDLEKMIKQCQKDNVKLGLAIKPNTQVEEIRQYLKHCSLVLIMSVEPGFGGQKFIENSFNKIKELADIRKALDLNFIIEVDGGVKDQNIAKCFECGVNLAVVGSYLVKNFSKETIDQLLK", "aa_hexdigest": "076d9189dc1522d1ae6cf59526ad953d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAB1", "query_cov": 1.0, "subject_cov": 0.8372093023255814, "identity": 0.9940000000000001, "score": 362.0, "evalue": 3.29e-126, "valid": true, "gene": "Rpe", "product": "Ribulose-phosphate 3-epimerase", "uniref50_id": "UniRef50_A0A448ZXM9", "cog_id": "COG0036", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXM9" ], "product": "D-ribulose-5-phosphate 3 epimerase" }, "genes": [ "rpe" ], "id": "DFOKDJOJIG_1206", "locus": "GCA013367855_01205" }, { "type": "cds", "sequence": "contig_1", "start": 689770, "stop": 690639, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S9", "UniRef:UniRef90_D1J8S9" ], "nt": "ATGAAAAAAGCAAATAAAATATTACTAGCTACATTTGCAACTACAATTTTAACATTACCTTTTGCCGCAATTTCTTGTAAAGTCGATCAAGTTAAAGAAAAAGAAAAATTAATAAACAATTCTAGTCTGCTAAATAAAGAAAACAAAAAAATATTATTAAATGAATTAAACATATTAAAAACCCGTTTAGCAAATTCAAAACTTAATGAGAAACAAAAAAACGAATTTATTGAAATTCAACTATTTTTAAAATTAATAACACTTAAAGAACTAAAAATATCTAACGAATTATTAAAATATTATAAATTAATGGCCTTGCTTCAAGATAAGTCTGAAGTAAACAAATACGCAGATTTAATCAAAAAATATTCAAAAAAATTTTCTGATGTTTTAAAGGATGATAGTTCAATATTAAATGATATTTACAATCAAGTTGCAAATTTAATTCCTAGAATTAATTTACTTAAAAATTCTTATATTTTTTCATTAAATATAAATAATAAAAAATTCACTTTTTTGAATAATAATGGAATAGTATTCAATTTGGATTTTATATTGAATAACATTGAAAACAAGGAAAACAAATATTCAATTTATTACAATACAAAGAAATATGAACTAAATTTAGAAATAAAAAACATTGAATTTAATTCAAATATTAAAACTGCATTAGATTTTAAAAATCAAGATTTTTTAACAACTAATTTTTTGGATTATGATAAAGATTTAGTGTGCAATGAAATGTTAAAAAATGCAATGGTCTATAAAAATTATGAACTAGTTGAAGATTTTAATGAAAATGATAAGAACATTAAATTTTCAAAAATGTTTACTAGTCAAGATTTTGTAAATAATTATTTGGTTAATTGA", "aa": "MKKANKILLATFATTILTLPFAAISCKVDQVKEKEKLINNSSLLNKENKKILLNELNILKTRLANSKLNEKQKNEFIEIQLFLKLITLKELKISNELLKYYKLMALLQDKSEVNKYADLIKKYSKKFSDVLKDDSSILNDIYNQVANLIPRINLLKNSYIFSLNINNKKFTFLNNNGIVFNLDFILNNIENKENKYSIYYNTKKYELNLEIKNIEFNSNIKTALDFKNQDFLTTNFLDYDKDLVCNEMLKNAMVYKNYELVEDFNENDKNIKFSKMFTSQDFVNNYLVN", "aa_hexdigest": "0e49d7c08133d2c1c1d837a649e275a6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 689770, "stop": 690891, "observations": { "mutation": [ 690637 ], "stop": [ 690637 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S9", "query_cov": 1.0, "subject_cov": 0.774798927613941, "identity": 0.976, "score": 527.0, "evalue": 3.46e-187, "gene_start": 1, "gene_end": 289, "reference_start": 1, "reference_end": 289, "reference_sequence": "MKKANKILLSTFATTILALPFATISCKVDQVKEKEKLINNSSQLNKENKKILLNELNILKTRLVNSKLNEKQKNEFIEIQLFLKLITLKELKISNELLKYYKLMALLQDKSEVNKYADLIKKYSKKFSDVLKDDSSILNDIYNQVANLIPRINLLKNSYIFSLNINNKKFTFLSNNGILFNLDFILNNIENKENKYSIYYNTKKYELNLEIKNIEFNSNIKTALDFKNQDFLTTNFLDYDKDLVCNEMLKNAMVYKNYELVEDFNENDKNIKFSKMFTSQDFVNNYLVNWLTNEPNICQIGEDKFLIYYHLPFSIENKKIVQKPSLYISNVVADSTIIKAIASRNVYLLKNLFLLLPKENYNENLKVLNNLMK" }, "paralog": false, "identity": 0.9785522788203753, "score": 716.0, "evalue": 6.45e-264, "description": "Internal stop codon at 690637. Nonsense mutation around 690637.", "psc": { "uniref90_id": "UniRef90_D1J8S9", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J8S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S9", "UniRef:UniRef50_D1J8S9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S9" ], "product": "Lipoprotein" } }, "id": "DFOKDJOJIG_1207", "locus": "GCA013367855_01206" }, { "type": "cds", "sequence": "contig_1", "start": 691279, "stop": 691818, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F067" ], "nt": "ATGTATAAGAAAACAAATAGAAAAATATTGATTTCTCTTGCCGTATTATGTGGAGGTTTAGCGGTGGCATCAATTGCTACAGTTTCTGCATATGCGGGTAAATCAAGACCAGAATCAAAAATAGAAAAAGAACATCTTTTGAATGACTTTAAAGAGGCATTAAATCAATTAAAACAATTGATTGCATCACCAGAAGCTAAAAATATTGAAAAATCAATGGAAATCAATATCTTAAATAATGCCAATGTTTCAGAAAATTCTTCAATTAAAGAAATTCAATCTAAAATCAATCAAGTTAAATATGCTATTGATTCATTAAACAAAAAAATCGCAGATCGAAATAAAAAGGAATTTGAAAAATTTGAACAAATCAAAAATGATCTTCAAACTTATATTAATAGAGATTTAAATGTTGTTATATACAATGAAATAACTAAAAAATCTAAAAATGAAATATTGAAATTGAATAGCATATCGCAAAGCTCAAAAGAACAAGAAATTATAGATGCAATAAGCAAATTGTATGATTTAAAGAAATGA", "aa": "MYKKTNRKILISLAVLCGGLAVASIATVSAYAGKSRPESKIEKEHLLNDFKEALNQLKQLIASPEAKNIEKSMEINILNNANVSENSSIKEIQSKINQVKYAIDSLNKKIADRNKKEFEKFEQIKNDLQTYINRDLNVVIYNEITKKSKNEILKLNSISQSSKEQEIIDAISKLYDLKK", "aa_hexdigest": "20911e1d85d5466874407efd0232eb8e", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001939F067", "query_cov": 1.0, "subject_cov": 0.8731707317073171, "identity": 0.799, "score": 242.0, "evalue": 7.37e-79, "valid": false, "uniref50_id": "UniRef50_UPI001939F067" }, "pscc": { "uniref50_id": "UniRef50_UPI001939F067", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F067" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20345.228600000013, "isoelectric_point": 9.372236442565917 }, "id": "DFOKDJOJIG_1208", "locus": "GCA013367855_01207" }, { "type": "cds", "sequence": "contig_1", "start": 691882, "stop": 692385, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001594B72E", "UniRef:UniRef90_UPI001594B72E" ], "nt": "TTGATTGAAATTGCTAATCAAATATTGTTATCAAACAAAATAGTTGAACGTGACTTAGAATTTAAACAAGAAATTTCTAAGGCTGAGGCATCATTAAGTCAAACAAACAAAACTGCATCTGAACTTTTTAGAGAAAGAATATTGCTTCACAAATTTATTAATGAAACTCTATTACTTGAAGAAACAATGTTTAAAGAAAAGGAAACAAAAATTAACGAGCTTAAGAAACAATCAGAAACTATTTTTGAACATATTGGAAAAGACCCAAATAATAGCATATTCAATTATAATTCCCAATTTTATAGAAACATAATAACTAAAATTGATAGTTTGAACACTAATGAAAAAAGTTTAACCTTAAATAAAAAGATTGAAGATATAAGTAATGAAATAAATAATCTAGACAAATCATTTTCTAAGATGGTTTCTGATACCCTTGAAAAATTTAAATATGAGGATGATCTTAAAGACCATATTGATGTTGCAAAAGATCCCAAAAATTGA", "aa": "MIEIANQILLSNKIVERDLEFKQEISKAEASLSQTNKTASELFRERILLHKFINETLLLEETMFKEKETKINELKKQSETIFEHIGKDPNNSIFNYNSQFYRNIITKIDSLNTNEKSLTLNKKIEDISNEINNLDKSFSKMVSDTLEKFKYEDDLKDHIDVAKDPKN", "aa_hexdigest": "73c6f77982255d77d5bf2789aa4205e7", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 691735, "stop": 692425, "observations": { "insertion": [ 692356 ], "mutation": [ 691816 ], "stop": [ 691816 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001594B72E", "query_cov": 0.9940119760479041, "subject_cov": 0.7248908296943232, "identity": 0.9279999999999999, "score": 280.0, "evalue": 6.24e-94, "gene_start": 1, "gene_end": 166, "reference_start": 50, "reference_end": 215, "reference_sequence": "MKLNSISQSSKEQEIIDAISKLYDLKKWILAQKLLIDNSINEQKRQLKSLIEIANQILLSNKIVERDLEFKQEISKAEASLSQTNKTTSELFRERILLHKFINETLLLEEKMLKEKETKINELKKQSETIFEHIGKDPNNSIFYYNSQFYRNIITKIDSLNTNEKSLTLNKKIEDISNEINNLDKSFSKLVSDTLEKFEYEDDLKDHMMLQKIPKIELIFKNISDTLGQ" }, "paralog": false, "identity": 0.9606986899563319, "score": 414.0, "evalue": 4.5e-149, "description": "Internal stop codon at 691816. Frameshift due to insertion around 692356. Nonsense mutation around 691816.", "psc": { "uniref90_id": "UniRef90_UPI001594B72E", "uniref50_id": "UniRef50_UPI001594B72E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001594B72E", "UniRef:UniRef50_UPI001594B72E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001594B72E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001594B72E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19604.9714, "isoelectric_point": 5.3773893356323255 }, "id": "DFOKDJOJIG_1209", "locus": "GCA013367855_01208" }, { "type": "cds", "sequence": "contig_1", "start": 692668, "stop": 692895, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6", "UniRef:UniRef90_UPI00193AA5C6" ], "nt": "ATGCAAGTAGACCAAATAACAGATGAAATGAAAACAAAAATCTTTGATTTGTTAATTAAAGATTTATCTAATATTTTAAAGAAAGATAGTTCTGAAAGCAAACTATTAAGTGCAATATTGTTGCCTACAATATTTAAAAAGACATATGAAAATCTTTCTAAAATAATAAAGCCAATTATAGATATGATATATAATAAAGACGCATATAACATAGAAATTATTGGATGA", "aa": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSSESKLLSAILLPTIFKKTYENLSKIIKPIIDMIYNKDAYNIEIIG", "aa_hexdigest": "b296f2deeb65a7e41b94eb2e143ccfe8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 692668, "stop": 693198, "observations": { "mutation": [ 692893 ], "stop": [ 692893 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193AA5C6", "query_cov": 1.0, "subject_cov": 0.42134831460674155, "identity": 0.92, "score": 128.0, "evalue": 3.26e-36, "gene_start": 1, "gene_end": 75, "reference_start": 1, "reference_end": 75, "reference_sequence": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSFESKLLSAILLPTIFKKPYENLSKIVKPIIDMIHNKDEYSIEIIGWEKASQELSLDISNIKEAEKKALEIKAKLTQENLKNKLKEFKLVKENLGNLIKKASEKDIEVEEDTKFIYENSEEDENSTLYEVISSIIRIKKEIRYLKYKLD" }, "paralog": false, "identity": 0.9375, "score": 312.0, "evalue": 3.4e-111, "description": "Internal stop codon at 692893. Nonsense mutation around 692893.", "psc": { "uniref90_id": "UniRef90_UPI00193AA5C6", "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193AA5C6", "UniRef:UniRef50_UPI00193AA5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8662.175099999993, "isoelectric_point": 6.168133354187011 }, "id": "DFOKDJOJIG_1210", "locus": "GCA013367855_01209" }, { "type": "cds", "sequence": "contig_1", "start": 692977, "stop": 693204, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6", "UniRef:UniRef90_UPI00193AA5C6" ], "nt": "TTGACCCAAGAAAATTTAAAAAATAAATTAAAAGAATTTAAATTAGTAAAAGAAAACTTGGGAAATTTAATTAAAAAATTATCCGAAAAAGGCATCGAAGTCGAAGAAGATACAAAATTCATATATGAAAATTCTGAAGAAGACGAAAATTCTACATTATATGAAGTAATAAATTCTATTATTAGAATAAAAAAAGAAATAAGATACCTTGAATATAAACTAGATTAA", "aa": "MTQENLKNKLKEFKLVKENLGNLIKKLSEKGIEVEEDTKFIYENSEEDENSTLYEVINSIIRIKKEIRYLEYKLD", "aa_hexdigest": "59c68f3a7c1fff098279d7d5d9532771", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 692677, "stop": 693204, "observations": { "mutation": [ 692893 ], "stop": [ 692893 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193AA5C6", "query_cov": 1.0, "subject_cov": 0.42134831460674155, "identity": 0.9329999999999999, "score": 130.0, "evalue": 4.01e-37, "gene_start": 1, "gene_end": 75, "reference_start": 104, "reference_end": 178, "reference_sequence": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSFESKLLSAILLPTIFKKPYENLSKIVKPIIDMIHNKDEYSIEIIGWEKASQELSLDISNIKEAEKKALEIKAKLTQENLKNKLKEFKLVKENLGNLIKKASEKDIEVEEDTKFIYENSEEDENSTLYEVISSIIRIKKEIRYLKYKLD" }, "paralog": false, "identity": 0.9371428571428572, "score": 311.0, "evalue": 1.38e-110, "description": "Internal stop codon at 692893. Nonsense mutation around 692893.", "psc": { "uniref90_id": "UniRef90_UPI00193AA5C6", "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193AA5C6", "UniRef:UniRef50_UPI00193AA5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9013.191999999994, "isoelectric_point": 5.102345466613768 }, "id": "DFOKDJOJIG_1211", "locus": "GCA013367855_01210" }, { "type": "cds", "sequence": "contig_1", "start": 694198, "stop": 694548, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C8B9B7", "UniRef:UniRef90_UPI0005C8B9B7" ], "nt": "TTGCTAAAATCAATCTTCTTTAAATATCAACAATCCTTAAAGGTTTTTGCCTTAATTTCTTTAATTGAACTAGGAAGAATAGAAATTGAAGAGATTTGTGTGCCTTCAAATGCACCTTCTCCAATATTTTCAATACCATTTTTTAAAGTTAATTCCTTTAGATTTTTAAAGTTTTTAAAGGCTTCATTTTCTATTGATTTTACATTTTCAGGAATAGTAACAGAGGCAATTTTTTTAAGCCCCTTTGTTAAATCTTTAGTAATTTTGTCAAACCCATTATCAAATTTTATTTTGCAATTTTCAAAAGAAGCATTTTCATCTATTTCCTCTACCTTTGTGCCTTTAAAATAA", "aa": "MLKSIFFKYQQSLKVFALISLIELGRIEIEEICVPSNAPSPIFSIPFFKVNSFRFLKFLKASFSIDFTFSGIVTEAIFLSPFVKSLVILSNPLSNFILQFSKEAFSSISSTFVPLK", "aa_hexdigest": "5873cc51d06a7102b18a35535b8cd525", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0005C8B9B7", "query_cov": 1.0, "subject_cov": 0.9508196721311475, "identity": 0.905, "score": 187.0, "evalue": 1.64e-59, "valid": true, "uniref50_id": "UniRef50_UPI0005C8B9B7" }, "pscc": { "uniref50_id": "UniRef50_UPI0005C8B9B7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C8B9B7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13117.398399999982, "isoelectric_point": 9.46378154754639 }, "id": "DFOKDJOJIG_1212", "locus": "GCA013367855_01211" }, { "type": "cds", "sequence": "contig_1", "start": 694596, "stop": 694856, "strand": "-", "frame": 2, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019398118" ], "nt": "ATGTTATTAACTTTAGGACTTGTTACAACTTCAACAATCTGCTTTCCATTAGTGGCGGCATCATGTACTAAAACAACAGACAGCAATTCAAACAATCAAAATATAGATAAGAATAATGACAACAAACCTAATGAAAAAAAGCCAGAATCGTTAAAGAATGAAGATTCTTCTGCAAATAACGAAAATCAAAATGTTCAAGATAATAAAGAAAAAGAATTATATGAACTTTTTGATTTTGAAAGAAATTCTTCAAATAATTGA", "aa": "MLLTLGLVTTSTICFPLVAASCTKTTDSNSNNQNIDKNNDNKPNEKKPESLKNEDSSANNENQNVQDNKEKELYELFDFERNSSNN", "aa_hexdigest": "114c4e0326d183a095419aa0a39b1c09", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0019398118", "query_cov": 1.0348837209302326, "subject_cov": 0.9368421052631579, "identity": 0.753, "score": 77.0, "evalue": 1.1e-16, "valid": false, "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI0019398118" }, "pscc": { "uniref50_id": "UniRef50_UPI0019398118", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019398118" ], "product": "variable surface lipoprotein" }, "genes": [], "id": "DFOKDJOJIG_1213", "locus": "GCA013367855_01212" }, { "type": "cds", "sequence": "contig_1", "start": 695051, "stop": 695443, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07510.16" ], "nt": "ATGAAACCAAAAATGAAATCATTGCTTTTAAGTAGAATTCATTTCCTTTTATCTTGCGGAGAATACAATCAAAATAGATTATCCATCGGAGAAAAACAAATTAATTTAAATTTTGATTCAATATATTCTATATTTATTGACTCAAAATATGAATATGAGCACATAATAGCGCAAAATGATGCCGGCATAAATGCGTTGAGAGGAGAATCAAAAGATGAGGCAAAAAAATATATTGATATGATAGGCAATGGTTTTCAAATTCATGAAAAAATTAATAAGAAGGCTTCAAACAAAAACTTAATAGATAAATTTAAGGAATATAAAAAGTTAAATTTAGAAAGCAATATTTCTTTCTCGGGCAAAGGTTCTTATTTAATTAATTTAGAAAGTTGA", "aa": "MKPKMKSLLLSRIHFLLSCGEYNQNRLSIGEKQINLNFDSIYSIFIDSKYEYEHIIAQNDAGINALRGESKDEAKKYIDMIGNGFQIHEKINKKASNKNLIDKFKEYKKLNLESNISFSGKGSYLINLES", "aa_hexdigest": "3353353382a7227e1e491481e75fd7b9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07510.16", "name": "GmrSD_C", "length": 87, "aa_cov": 0.6692307692307692, "hmm_cov": 0.8505747126436781, "evalue": 2.4805255705287452e-11, "score": 39.298301696777344, "start": 38, "stop": 124 } ], "seq_stats": { "molecular_weight": 14985.988199999983, "isoelectric_point": 9.060273933410645 }, "id": "DFOKDJOJIG_1214", "locus": "GCA013367855_01213" }, { "type": "cds", "sequence": "contig_1", "start": 695466, "stop": 695807, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATTCATTTAATTTATCATTAATTTTTTTGTCAATATCAGAAGGATTATTTTTGAATCTTTTAAGTTCAGGAACAAGGTCAATTAATTGTTCTCTTAACTCATCAGGATTCTCAATTTCATCATTTTTTTTAATTTCATCAATAATTCGCGAAATTGATTTTGTTAATGATTGGCCTTCAAATGCAATCGTTTTTCAAACAAAATTAAATCTCTCTATTTCGCGTAATAAATTAAATGTATTATTTAAATCAAGATCTCTCATTGGTTTTTCATTATGTCAAATATTTAACTTATTAAATATTACTCATACTAATGGTATAAAAATTGTATTTTCTTGA", "aa": "MHSFNLSLIFLSISEGLFLNLLSSGTRSINCSLNSSGFSISSFFLISSIIREIDFVNDWPSNAIVFQTKLNLSISRNKLNVLFKSRSLIGFSLCQIFNLLNITHTNGIKIVFS", "aa_hexdigest": "c5ac14a8dbb6b69ab080df1e1f4393a3", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12624.52859999998, "isoelectric_point": 9.692579841613771 }, "id": "DFOKDJOJIG_1215", "locus": "GCA013367855_01214" }, { "type": "cds", "sequence": "contig_1", "start": 695774, "stop": 695962, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAAATATTTAAAAAATCCTAAAGATTTCTTTAATAGCTATAACTTGAATAATAAAGCTAAGAAGAAAAAAGACTCAAAATATGCCAAAGCATTAATTCAAAATGAGATTTTTTCACGTTCAGCATTTGCTGAGCAAGTTTATGCTGCTATTCAAGAAAATACAATTTTTATACCATTAGTATGA", "aa": "MYKYLKNPKDFFNSYNLNNKAKKKKDSKYAKALIQNEIFSRSAFAEQVYAAIQENTIFIPLV", "aa_hexdigest": "24b67972e502b6f95f6f82e62e35ee15", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7297.3468, "isoelectric_point": 9.698575401306151 }, "id": "DFOKDJOJIG_1216", "locus": "GCA013367855_01215" }, { "type": "cds", "sequence": "contig_1", "start": 696035, "stop": 696565, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTATTTATAGCAATATTGATGAAAATTTTGAATCAAACAACATTTATTCGTTTATAAATAAAACTATAAAAAATAATTTAAAAATTGAAACCAATGACAAAAATAATGATTTTGTTAAAAAACTTATAATTATTTTTAACAAACTTGAAAACTTAGAAACTAAGGGGCTTACTAATAAAATAGATTATGTTTTAAACAAATCTATAATCACATATACAATTGTTAAAACCAACAAGCCCGAAATTCTTTTTAAAAATCTTAACCTTAATTCAAAACCTTTAAATTCGCTAGATTTATTTAAAAATTACTTATATTCATTGGATCCAAAGAATCCAAATAACGAGGAAAACATAAAAATATTTAATGATGAATTTTATGAAGCATTAAGCAATACAAAAAACCAAATCAAGGAAGGTGAAGTTGAAAACTTTGCATACGTAATAAACAAAGTTGATTCTCAATATCAAATGGATAATGAAAAAAATTTAAACAAGAATGTGAATAATTTTCAACTACTTGAAAAATGA", "aa": "MSIYSNIDENFESNNIYSFINKTIKNNLKIETNDKNNDFVKKLIIIFNKLENLETKGLTNKIDYVLNKSIITYTIVKTNKPEILFKNLNLNSKPLNSLDLFKNYLYSLDPKNPNNEENIKIFNDEFYEALSNTKNQIKEGEVENFAYVINKVDSQYQMDNEKNLNKNVNNFQLLEK", "aa_hexdigest": "fe8f42c962c681b72430c165205a19d0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20739.185399999995, "isoelectric_point": 5.680170631408691 }, "id": "DFOKDJOJIG_1217", "locus": "GCA013367855_01216" }, { "type": "cds", "sequence": "contig_1", "start": 696845, "stop": 697063, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCAATGAAAGAATTACGACAATCCTTAATGAAGAAATTTTAAAAAATAATTTTTCTATTGAAAATAATTCTCAAGAAGATTACCAAAATTCAAAATGTCAAGGAAATAATAAATTAAATGATGAAATTTATAGTATTCATTTAACAATATTTAGTTTGTTAAATGATTATTTCGAAAATGTAATAATTCCTATTTTTCAAAGAAAATATGTTTGA", "aa": "MPNERITTILNEEILKNNFSIENNSQEDYQNSKCQGNNKLNDEIYSIHLTIFSLLNDYFENVIIPIFQRKYV", "aa_hexdigest": "97f2e29089ae86720ba4aa2a714d42b7", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8585.513799999997, "isoelectric_point": 4.71595401763916 }, "id": "DFOKDJOJIG_1218", "locus": "GCA013367855_01217" }, { "type": "cds", "sequence": "contig_1", "start": 697082, "stop": 697579, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGTTGCAATCTATTTTAATATATTAATTAAAATAAACAAAAATGAATACGAATTAACACCCGATTTTATTGAATGTGTTGTTCAAAAAAATAATTCAAATAAAAATAATGTAAAGCTATTCTTAAATGATTTTTTATTAAACCAAATTAATAATAAATTTGAAACTCTTACCGAAATCATTAAAACTTATGATAAAAATGTTAATGAATCTCTTCGCGAAAACCAAATTAAAGGAATAAATTGTGTTGAAGATTCTAATATTTCGTTTTCTTTTTTAGTCAAATTAAACAAATTATTCAAAAAAAACAAAAAAGATGAAGAACAAATTAACAAAGATATTAAGCTAATTAAATCCGAATTTAAATCTATAAGAAATAGTAAAGATAACAAGGACGACTATTTTGGACCTATATTAGAAGAAATTGCTTCAGAATATAGCAAAGATGATTTGTTAAATTCAATAAAAAATTCTTTCTATTCGATTGTTAATTGA", "aa": "MKVAIYFNILIKINKNEYELTPDFIECVVQKNNSNKNNVKLFLNDFLLNQINNKFETLTEIIKTYDKNVNESLRENQIKGINCVEDSNISFSFLVKLNKLFKKNKKDEEQINKDIKLIKSEFKSIRNSKDNKDDYFGPILEEIASEYSKDDLLNSIKNSFYSIVN", "aa_hexdigest": "cd78baeb4b2552f9f41948fd9553674a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19407.909599999995, "isoelectric_point": 7.621563148498535 }, "id": "DFOKDJOJIG_1219", "locus": "GCA013367855_01218" }, { "type": "cds", "sequence": "contig_1", "start": 698940, "stop": 699554, "strand": "+", "frame": 3, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D2IMT5" ], "nt": "ATGGCTAAAGAATTTATCTTAGGTATAGACTTAGGTACTACTAACTCGGTAGTATCTGTTATAGAAAATGGAACCCCAAAAATATTAGAAAATCCAAATGGTAAAAGAACTACACCATCTGTTGTTGCTTTTAAAAATGGAGAAACAATTATTGGTGAAAGTGCTAAAAGACAATTGGAATCAAATAAAGATAGTGTTGCATCTATAAAAAGATTAATGGGTACTTCTCAAACAGTACATCTAAATAATAAAGATTACAAACCTGAAGAAATCAGCGCAATGATTTTAAGTTACATGAAAGATTATGCTGACAAAAAATTAGGTCAACCTGTTAAAAAAGCAGTTATCACAGTTCCAGCATATTTTGACAATGCACAACGTGAAGCAACAAAGAATGCGGGAATTATTGCTGGTCTTGATGTTGTAAGAATTATTAACGAACCCACTGCTGCCGCATTGGCATTTGGATTGAATAAAGATAAAAATGAAAATCAAAAGATCTTAGTATTTGACTTAGGTGGTGGTACATTTGACGTTTCATTGCTTGAAATGGAAAGTGGAACATTTGAAGTTTTGGCAACAGCAGGCGATAACCACTTAGGTGGTGATGACTGA", "aa": "MAKEFILGIDLGTTNSVVSVIENGTPKILENPNGKRTTPSVVAFKNGETIIGESAKRQLESNKDSVASIKRLMGTSQTVHLNNKDYKPEEISAMILSYMKDYADKKLGQPVKKAVITVPAYFDNAQREATKNAGIIAGLDVVRIINEPTAAALAFGLNKDKNENQKILVFDLGGGTFDVSLLEMESGTFEVLATAGDNHLGGDD", "aa_hexdigest": "8ddb54cc4cdd33d560f0071fd07de60e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002E1EEFC2", "query_cov": 0.9754901960784313, "subject_cov": 0.9660194174757282, "identity": 0.6729999999999999, "score": 264.0, "evalue": 4.31e-87, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_A0A3D2IMT5" }, "pscc": { "uniref50_id": "UniRef50_A0A3D2IMT5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D2IMT5" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "DFOKDJOJIG_1220", "locus": "GCA013367855_01219" }, { "type": "cds", "sequence": "contig_1", "start": 699576, "stop": 699851, "strand": "+", "frame": 3, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9D4" ], "nt": "ATGGTTGAACAAATTAAATCTAAATACAATTTCGATCCAACAACCGACAAAATGGCAATGGCAAGATTAAAAGAAGAAGCAGAACGTGCAAAAATTACTTTATCAGAACAATTAATTGCAAACATTTCTCTTCCATTCTTAGCAATGAATGAAAATGGACCAGTTAATGTTGAATTAGAAATTACTCGTGCTACATTTGAATCAATGACTGAACATTTACTACAAAGAACAAGAAAACCATTATTAGATGTTTTAAGTGAAGCAAAATTAACATGA", "aa": "MVEQIKSKYNFDPTTDKMAMARLKEEAERAKITLSEQLIANISLPFLAMNENGPVNVELEITRATFESMTEHLLQRTRKPLLDVLSEAKLT", "aa_hexdigest": "31f1a5a70ac54c463adff44b9808218f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387B89B0", "query_cov": 0.9340659340659341, "subject_cov": 0.8252427184466019, "identity": 0.588, "score": 106.0, "evalue": 3.29e-28, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_A0A223M9D4" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9D4" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "DFOKDJOJIG_1221", "locus": "GCA013367855_01220" }, { "type": "cds", "sequence": "contig_1", "start": 699867, "stop": 700727, "strand": "+", "frame": 3, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_Q49539", "UniRef:UniRef90_Q9ZEJ0" ], "nt": "GTGTTACTAGTTGGTGGTTCAACAAGAATGCCAGCTGTTCAAAAATTAGTAGCAGAAGTAACTAATAAAAAACCTAATAATTCAATTAACCCTGATGAAGTAGTTAGCGTTGGAGCTGCTATTCAAGGTGCAATATTAGCAGGAGAAATTCAAGATGTTTTACTACTTGATGTTACTCCTTTAACATTAGGCATTGTAGTAGAAGGTGATGTTGTAGCGCCTTTAATTCCAAGAAATACAACAATTCCTGTTACAAAGAGTCAAATATTCTCAACAGCTGTTGATAATCAAACAGCAGTAACAATCGTTATCACACAAGGTGAAAGACAATTGGCTAGAGATAACAAGATTTTGGGTCAATTCAATCTTGAAGGAATTGAACCTGCGCCAAGAGGAATTCCTCAAATTGAAGTAAGTTTCTCAATTGACGTAAATGGTATTACTAAAGTAACAGCAAAAGACAAAAAGACAAACAAAGAACAAACAATAACCATTCAAAACACCTCTTCATTATCAAAAGAAGAAGTAGAAAAGATGGTTAAGGACGCAGAAGCAAATAGAGAAGCTGATCAAAAGAAACGTCACGAAATCGAAGTTATTGTAAAAGCAGAACAATTAAGCAACGACTTAGAAAAGACATTAAAATCAGAACAAGCTAAAAACCTAGGCGAACCTCAAAAACAAGAACTTCAAAAAGAAATAGATGAAATTAAAGAACTAATAAATAAAAAAGATATCGAACAACTAGAAAAGAAAATTACAGAATTTGAACAAAAAATGGCTCAAGCTGCTGAATTTTTAAAGAAACAACAAGGAAATAATAATCCAAACACCAACAACGACAACCCTCAAACTAACTAA", "aa": "MLLVGGSTRMPAVQKLVAEVTNKKPNNSINPDEVVSVGAAIQGAILAGEIQDVLLLDVTPLTLGIVVEGDVVAPLIPRNTTIPVTKSQIFSTAVDNQTAVTIVITQGERQLARDNKILGQFNLEGIEPAPRGIPQIEVSFSIDVNGITKVTAKDKKTNKEQTITIQNTSSLSKEEVEKMVKDAEANREADQKKRHEIEVIVKAEQLSNDLEKTLKSEQAKNLGEPQKQELQKEIDEIKELINKKDIEQLEKKITEFEQKMAQAAEFLKKQQGNNNPNTNNDNPQTN", "aa_hexdigest": "21c036ad0da62f0e522370c355b68e2d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_W6TIT3", "query_cov": 0.9755244755244755, "subject_cov": 0.8584615384615385, "identity": 0.509, "score": 243.0, "evalue": 6.33e-76, "valid": false, "product": "Chaperone protein dnaK", "uniref50_id": "UniRef50_W6TIT3" }, "genes": [ "dnaK" ], "truncated": "5-prime", "pseudogene": { "start": 699567, "stop": 700727, "observations": { "mutation": [ 699573, 699849 ], "stop": [ 699573, 699849 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZEJ0", "query_cov": 0.9965034965034965, "subject_cov": 0.4789915966386555, "identity": 1.0, "score": 509.0, "evalue": 9.15e-177, "gene_start": 2, "gene_end": 286, "reference_start": 311, "reference_end": 595, "reference_sequence": "MAKEFILGIDLGTTNSVVSVIENGTPKILENPNGKRTTPSVVAFKNGETIIGESAKRQLESNKDSVASIKRLMGTSQTVHLNNKDYKPEEISAMILSYMKDYADKKLGQPVKKAVITVPAYFDNAQREATKNAGIIAGLDVVRIINEPTAAALAFGLNKDKNENQKILVFDLGGGTFDVSLLEMESGTFEVLATAGDNHLGGDDWDHEIVKWMVEQIKSKYNFDPTTDKMAMARLKEEAERAKITLSEQLIANISLPFLAMNENGPVNVELEITRATFESMTEHLLQRTRKPLLDVLSEAKLTWNDINEVLLVGGSTRMPAVQKLVAEVTNKKPNNSINPDEVVSVGAAIQGAILAGEIQDVLLLDVTPLTLGIVVEGDVVAPLIPRNTTIPVTKSQIFSTAVDNQTAVTIVITQGERQLARDNKILGQFNLEGIEPAPRGIPQIEVSFSIDVNGITKVTAKDKKTNKEQTITIQNTSSLSKEEVEKMVKDAEANREADQKKRHEIEVIVKAEQLSNDLEKTLKSEQAKNLGEPQKQELQKEIDEIKELINKKDIEQLEKKITEFEQKMAQAAEFLKKQQGNNNPNTNNDNPQTN" }, "paralog": false, "identity": 0.9948186528497409, "score": 730.0, "evalue": 3.38e-266, "description": "Internal stop codon at 699573, 699849. Nonsense mutation around 699573, 699849.", "psc": { "uniref90_id": "UniRef90_Q9ZEJ0", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_Q49539", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZEJ0", "UniRef:UniRef50_Q49539", "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_Q49539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49539" ], "product": "Chaperone protein DnaK" } }, "id": "DFOKDJOJIG_1222", "locus": "GCA013367855_01221" }, { "type": "cds", "sequence": "contig_1", "start": 700949, "stop": 701251, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGACAGCTTTAAACAATTATCACAATTTTTTATTTAAAGTTACTGCTAAGAAAAAAAGCACAATAATTATTCCTATAATTTTATTGTTATGCAGTTTGATTTTGTGTTTTGTTTTTGTTGGTACTAAACCAGCACCGCGTTATTTTAATGTAATTATTTTTGCATATACATTAGTTGCAATATTATTTACAGTATTATATGGCTCTCTCAAGTCCTTAAATATATTTAAAGATCTTGAACAAGATGGGATTGAATTAATTATATTTTCTAAGCCCATTTCAAGAAAAGCAATAATCTGA", "aa": "MKTALNNYHNFLFKVTAKKKSTIIIPIILLLCSLILCFVFVGTKPAPRYFNVIIFAYTLVAILFTVLYGSLKSLNIFKDLEQDGIELIIFSKPISRKAII", "aa_hexdigest": "13c2c510db0c8a1b34ed134faf46f4eb", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11355.716099999987, "isoelectric_point": 9.781288337707515 }, "id": "DFOKDJOJIG_1223", "locus": "GCA013367855_01222" }, { "type": "cds", "sequence": "contig_1", "start": 701294, "stop": 701707, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E50136B", "UniRef:UniRef90_UPI001E50136B" ], "nt": "TTGTTTGCTTTTGTTAGTTCTATTATTGTATATAGTCAAGTTTCAAAAGGAAACATGTTTGGATATTTAGTTTTGTTGTCATTGGTTGCGCATTTTCTTGCATATACAATTTTTGGATATATAGCAGCATTGATTGCATATAAAGTTAACCAAAAAATTGCTATAACTGTTCCAATCATAATTTTTGCACCTATGGCAATAGGTGGAGGATTTATATTCGCCAACTCAACATCAACAAATGAAAATTTTGCTCATTATATTAATAGCAAATATAAATACCACAGAGCAGGAAACGAAGTTAATAGTGAAGTATTTTATTTAAATAAAAATGATGATAAATATTATTTAGTTCCAAATGGAATTAATAATAACAAGTTTAGTGATGTACAAAATCAATATTTAAATTTAGCCTGA", "aa": "MFAFVSSIIVYSQVSKGNMFGYLVLLSLVAHFLAYTIFGYIAALIAYKVNQKIAITVPIIIFAPMAIGGGFIFANSTSTNENFAHYINSKYKYHRAGNEVNSEVFYLNKNDDKYYLVPNGINNNKFSDVQNQYLNLA", "aa_hexdigest": "b359e4c38045550671456b0d50933864", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 700994, "stop": 701835, "observations": { "deletion": [ 701825 ], "mutation": [ 701249, 701288, 701705, 701750 ], "stop": [ 701249, 701288, 701705, 701750 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E50136B", "query_cov": 1.0, "subject_cov": 0.46440677966101696, "identity": 0.993, "score": 260.0, "evalue": 1.86e-85, "gene_start": 1, "gene_end": 137, "reference_start": 116, "reference_end": 252, "reference_sequence": "MKTALNNYHNFLFKVTAKKKSTIIIPIILLLCSLILCFVFVGTKPAPRYFNVIIFAYTLVAILFTVLYGSLKSLNIFKDLEQDGIELIIFSKPISRKAIIWGKILSFNSLGLIWTLFAFVSSIIVYSQVSKGNMFGYLVLLSLVAHFLAYTIFGYIAALIAYKVNQKIAITVPIIIFAPMAIGGGFIFANSTSTNENFAHYINSKYKYHRAGNEVNSEVFYLNKNDDKYYLVPNGINNNKFSDVQNQYLNLAWKYSNSSANEWQKYSWLAMPYQFVDIFNIENQNIFSNLSSRQY" }, "paralog": false, "identity": 0.9857142857142858, "score": 531.0, "evalue": 1.18e-194, "description": "Internal stop codon at 701249, 701288, 701705, 701750. Frameshift due to deletion around 701825. Nonsense mutation around 701249, 701288, 701705, 701750.", "psc": { "uniref90_id": "UniRef90_UPI001E50136B", "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI001E50136B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E50136B", "UniRef:UniRef50_UPI001E50136B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E50136B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E50136B" ], "product": "ABC transporter permease" } }, "id": "DFOKDJOJIG_1224", "locus": "GCA013367855_01223" }, { "type": "cds", "sequence": "contig_1", "start": 701759, "stop": 702121, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATATCAATTTGTTGATATATTTAACATTGAAAATCAAAATATTTTTAGCAACCTTTCTTCAGACAGTATTAATAATAGTTTAAGTAATTATTTATATTATAAAAATCAAGAAAATACAATATATAAATATAAATTAAATAGAAATTCGGACTTATTAAAATTACCAACATTAGAAAATAAAATTAAGATTAATAAATACTATGTCCCAGGAATGTTAAATTTAAATTCAAATATTGATAATGAAGTAAATAATGGAGTAATTTACGCTAGAAAAAATGCTTCTAATTTTTCTGCCTCTTTTGAAGAAGACAACTTTGTTTATGCATCTCCAAATAATCTAGTAGGAAAATTAAAATGA", "aa": "MPYQFVDIFNIENQNIFSNLSSDSINNSLSNYLYYKNQENTIYKYKLNRNSDLLKLPTLENKIKINKYYVPGMLNLNSNIDNEVNNGVIYARKNASNFSASFEEDNFVYASPNNLVGKLK", "aa_hexdigest": "9cb12b48790108dc19c59498f8d7dac3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13924.404799999982, "isoelectric_point": 7.826155662536623 }, "id": "DFOKDJOJIG_1225", "locus": "GCA013367855_01224" }, { "type": "cds", "sequence": "contig_1", "start": 702128, "stop": 702715, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5A3247", "UniRef:UniRef90_UPI001E5A3247" ], "nt": "ATGAGCCAAGTTTTAAAATATCATAAATTTACAGAAGCAGCCGAAGAGTTTTATTCTAAGGTTAAAAATGAAATTAGTTTAAAGAAATTAGTTAAACCATATGATATTAAAAAGCTATTGTTAGAAACGATTTCAAATGAAGTTAATAATACAAATAGTAAGTTCTTTAATATAGATAATGTTAACTTAGCAGTATTCGACCCTCATGGTGTTAGAGATGGAAGATTACAATCTGAAACAGAAAGAAAAATTTATATTGCCACAGCGTTGCTATATTTTATCTATTTTAAAGATAATCAAGGTCAAGTTATTAAATCATTATTGAAATCTGAAAGTGATAAAACAACATATGCACCAAGCCAAATAGAAATCAAGGTAGATGGTTATATCTATGAAATAGGTGGATATGAAAGCTACGAACCAAAACAACAAGTTCAAGAAAATAAAATAACTAAAAATAAACAAGTAGTAGTTAGATATGAATTAATTCCAAGTAATAAAAATTATTTATTCCAAACAGTAGATGATGTATATTCAATTGAAAGAGATAGAAAAATAGTTAATAAACAATTTTATATTTTAATTTGA", "aa": "MSQVLKYHKFTEAAEEFYSKVKNEISLKKLVKPYDIKKLLLETISNEVNNTNSKFFNIDNVNLAVFDPHGVRDGRLQSETERKIYIATALLYFIYFKDNQGQVIKSLLKSESDKTTYAPSQIEIKVDGYIYEIGGYESYEPKQQVQENKITKNKQVVVRYELIPSNKNYLFQTVDDVYSIERDRKIVNKQFYILI", "aa_hexdigest": "4955eabfddec0c812b5c3117958ac4fd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 701828, "stop": 702781, "observations": { "mutation": [ 702119, 702713 ], "stop": [ 702119, 702713 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E5A3247", "query_cov": 1.0, "subject_cov": 0.5571428571428572, "identity": 0.99, "score": 372.0, "evalue": 1.04e-127, "gene_start": 1, "gene_end": 195, "reference_start": 134, "reference_end": 328, "reference_sequence": "MNDKNIHDLQCHINLLIYSTLKIKIFLVTFLRDNINNSLSNYLYYKNQENTIYKYKLNRNSDLLKLPTLENKTKINKYYVPGMLNLNSNIDNEVNNGVIYARKNASNFSASFEEDNFVYASPNNLVGKLKWKIMSQVLKYHKFIEAAEEFYSKVKNEISLKKLVKPYDIKKLLLEMISNEVNNTNSKFFNIDNVNLAVFDPHGVRDGRLQSETERKIYIATALLYFIYFKDNQGQVIKSLLKSESDKTTYAPSQIEIKVDGYIYEIGGYESYEPKQQVQENKITKNKQVVVRYELIPSNKNYLFQTVDDVYSIERDRKIVNKQFYILIWIVVAIILMMANRFLYLRKDYK" }, "paralog": false, "identity": 0.9779179810725552, "score": 612.0, "evalue": 9.13e-225, "description": "Internal stop codon at 702119, 702713. Nonsense mutation around 702119, 702713.", "psc": { "uniref90_id": "UniRef90_UPI001E5A3247", "uniref50_id": "UniRef50_UPI001E5A3247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E5A3247", "UniRef:UniRef50_UPI001E5A3247" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E5A3247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5A3247" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 22920.87280000001, "isoelectric_point": 8.748956108093264 }, "id": "DFOKDJOJIG_1226", "locus": "GCA013367855_01225" }, { "type": "cds", "sequence": "contig_1", "start": 702781, "stop": 703485, "strand": "+", "frame": 1, "gene": "rbbA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1131", "COG:JV", "RefSeq:WP_020002508.1", "SO:0001217", "UniParc:UPI000365E072", "UniRef:UniRef100_A0A454CAA1", "UniRef:UniRef50_A0A449B6T2", "UniRef:UniRef90_D1J8T4" ], "nt": "ATGGATAATCATATTTTAGAATTAAAAAATGTAGTAAAAATCTATAATAAATCAGATCGTGGAATTCATAATATTTCATTTGCTGTCGAATCTGGAAATTTTCATGCATTTATTGGCGAAAATGGTGCGGGAAAAACAACAACAATTAAAACAATAATTGGTTCTTATATAAATTTTAAAGGTGAAATACTTATTGGCGGAATTACAAACTTAAACCCTAATTCTAAAAAAATTCTTGGTTATGTTCCAGAAAATGCAGTGTTTCCTAAAGAACTAACAACATATGAATATTTAAAGGCCCTTGCATTATTATCAGGAATAAATAAAACCGAAGTAAAGGCAAAAATCGAAAGTTATTTACAAAGATTTGGAATTATTGAATTAAAAAACGAAAAGCCCGCTAATTTTTCATCAGGTCAAAAGAAAAAAGTTTTATTAATACAAGCTTTAATTAACGAGCCTAAATTATTGATTTTAGATGAACCAGCTGCTAATTTAGACCCTACTGCAAGATTTGAATTGTTTTCAATATTACATGAACTAAATGAAAAAGGAACAACAATTTTTATTAGTTCCCACGTTCTAAGTGAAATTGATAAATATGCAAATTCATTAACATTAATTCATGGCGGAAAGATCTTATATTCTGGCCCTAAATATGATTCATTGGAAAAAATATTTTATGAAAAAGTTATTAAAAATTAG", "aa": "MDNHILELKNVVKIYNKSDRGIHNISFAVESGNFHAFIGENGAGKTTTIKTIIGSYINFKGEILIGGITNLNPNSKKILGYVPENAVFPKELTTYEYLKALALLSGINKTEVKAKIESYLQRFGIIELKNEKPANFSSGQKKKVLLIQALINEPKLLILDEPAANLDPTARFELFSILHELNEKGTTIFISSHVLSEIDKYANSLTLIHGGKILYSGPKYDSLEKIFYEKVIKN", "aa_hexdigest": "cbe68b00230783e80869ce26faa8a7ac", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000365E072", "ncbi_nrp_id": "WP_020002508.1", "uniref100_id": "UniRef100_A0A454CAA1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000365E072", "RefSeq:WP_020002508.1", "UniRef:UniRef100_A0A454CAA1" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CAA1", "uniref90_id": "UniRef90_D1J8T4" }, "psc": { "uniref90_id": "UniRef90_D1J8T4", "gene": "RbbA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A449B6T2", "cog_id": "COG1131", "cog_category": "JV" }, "pscc": { "uniref50_id": "UniRef50_A0A449B6T2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B6T2" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "rbbA" ], "id": "DFOKDJOJIG_1227", "locus": "GCA013367855_01226" }, { "type": "cds", "sequence": "contig_1", "start": 703463, "stop": 703855, "strand": "+", "frame": 2, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A573B35", "UniRef:UniRef90_UPI000A573B35" ], "nt": "ATGAAAAAGTTATTAAAAATTAGTTTGTCATTGCCGTTAGTTATTGCAACAATTCCATTAGTTGCTTCAAGCTGCAAACAAACAAATATTGAAATTAAAAAACCAGATGATAAAAAAGAAACAGCAAATCAAATCCAAGCAAAAATTAATAAAGAATTTCAAAAAAATAAAGTGGTTCAAAAATTACTAAGTTCATTTATTGAAAATGAAAAAGAACAAGATATTTTTGTACAAAATCAAAACAATATATTGCCCAATGTATATTCAGAACTAAAGTTTTCTTTGTTGTATTTCCCTATTTATAATAGAGCCTCAGATAATTCGGGTAGCGAACTTTATGGATTTATAAGACAATCAAGAGAAATTATTGAAAACACATTGCAAAGAAATTGA", "aa": "MKKLLKISLSLPLVIATIPLVASSCKQTNIEIKKPDDKKETANQIQAKINKEFQKNKVVQKLLSSFIENEKEQDIFVQNQNNILPNVYSELKFSLLYFPIYNRASDNSGSELYGFIRQSREIIENTLQRN", "aa_hexdigest": "c2b0bb12fa5f8f5d03b273e17e777d4f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 703463, "stop": 703988, "observations": { "insertion": [ 703937 ], "mutation": [ 703853, 703859 ], "stop": [ 703853, 703859 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A573B35", "query_cov": 1.0, "subject_cov": 0.7471264367816092, "identity": 0.992, "score": 240.0, "evalue": 1.5e-79, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MKKLLKISLSLPLVIATIPLVASSCKQTNIEIKKPDDKKETANQIQAKINKEFQKNKVVQKLLSSFIENEKEQDIFVQNQNNILPNVYSELKFSLLYFPIYNRASDNPGSELYGFIRQSREIIENTLQRNWYWYLKQIGNFVYSINPYNDRYIETSQTKKQFNLPKKTLVFYIK" }, "paralog": false, "identity": 0.9770114942528736, "score": 327.0, "evalue": 5.32e-116, "description": "Internal stop codon at 703853, 703859. Frameshift due to insertion around 703937. Nonsense mutation around 703853, 703859.", "psc": { "uniref90_id": "UniRef90_UPI000A573B35", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI000A573B35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A573B35", "UniRef:UniRef50_UPI000A573B35" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A573B35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A573B35" ], "product": "aromatic motif membrane protein" } }, "id": "DFOKDJOJIG_1228", "locus": "GCA013367855_01227" }, { "type": "cds", "sequence": "contig_1", "start": 703997, "stop": 704392, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE", "UniRef:UniRef90_UPI000A5C6EDE" ], "nt": "ATGCCTAAGGATATTATTGAATTGCCTTATAACCCAATAAGTTCAGATGAATTAAAAGAAATAGTTGCTAAAAAAATTAATGAAAGTATTGATTCTAAAGTTCAAGACCAATTTAGCGATTATTTAGAAGATATAATTAATGATTCATCAAAGTTAAAAATAGATACAAATTCAGAAATAACCAATTTTTTAAGAAACAAAATTAACTTAAATGATGCAAAAAAGCTAATAGAAAATTATTTGAACAATAAGAACAAATTTGAAACAAGTAATGAATATCAAATTAATAACGAAATTTCTAATAAAATATTTAATCTTTTAAATACAAAAGGATCTTCATTTGATAACTATTCCTCAAAGAAAATCTTTTACGTAGCATACGACCACAAAACTTGA", "aa": "MPKDIIELPYNPISSDELKEIVAKKINESIDSKVQDQFSDYLEDIINDSSKLKIDTNSEITNFLRNKINLNDAKKLIENYLNNKNKFETSNEYQINNEISNKIFNLLNTKGSSFDNYSSKKIFYVAYDHKT", "aa_hexdigest": "8c41d3c145a4ee4ce1ffeaefa5087da6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 703997, "stop": 704695, "observations": { "mutation": [ 704390 ], "stop": [ 704390 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "query_cov": 1.0, "subject_cov": 0.5282258064516129, "identity": 0.9470000000000001, "score": 231.0, "evalue": 7.98e-75, "gene_start": 1, "gene_end": 131, "reference_start": 1, "reference_end": 131, "reference_sequence": "MPKDIIELPYNPISSDELKEIVAKKINESIDSKVQNQFSDYLEDRIDESSKLKIDINSEITNFLRNKINLNDAKKLIENYLNNKNKFEISNEYQINNEISKKIFNLLNTKGSSFDNYSSKKIFYVAYDHKTWIRVIVYTENNVKKISINPDVYILNQSNSYEQSKEILKKLNKQIFIERLNSIIDSLIENINEKDFNIKNFFEKNNDKKFFELFQKFSYNKYLIEAMQKINKEKDLIYRYSWETVDYE" }, "paralog": false, "identity": 0.9612068965517241, "score": 439.0, "evalue": 4.18e-159, "description": "Internal stop codon at 704390. Nonsense mutation around 704390.", "psc": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A5C6EDE", "UniRef:UniRef50_UPI000A5C6EDE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15281.88189999998, "isoelectric_point": 5.2209684371948235 }, "id": "DFOKDJOJIG_1229", "locus": "GCA013367855_01228" }, { "type": "cds", "sequence": "contig_1", "start": 704402, "stop": 704743, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE", "UniRef:UniRef90_UPI000A5C6EDE" ], "nt": "ATGGTTTATACTGAAAATAACGTTAAAAAAATTTCTATAAATCCTGATGTTTATATTTTAAATCAAAGCAATAGCTATGAACAAAGTAAAGAAATTTTAAAAAAATTAAATAAACAAATTTTTATTGAACGGTTGAATTCAATTATTGATTCTTTAATTGAAAATATAAATGAAAAAGATTTCAATATTAAAAACTTTTTTGAAAAAAATAATGATAAAAAATTCTTTGAATTGTTTCAAAAGTTTTCATACAATAAATACTTGATTGAAGCAATGCAAAAAATTAATAAAGAAAAAGATCTAATTTATCGCTATAGTTGGGAGACAGTAGATTATGAATAA", "aa": "MVYTENNVKKISINPDVYILNQSNSYEQSKEILKKLNKQIFIERLNSIIDSLIENINEKDFNIKNFFEKNNDKKFFELFQKFSYNKYLIEAMQKINKEKDLIYRYSWETVDYE", "aa_hexdigest": "416f84b660550f6b0100828fee56ad81", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 704102, "stop": 704743, "observations": { "mutation": [ 704390 ], "stop": [ 704390 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "query_cov": 1.0, "subject_cov": 0.45564516129032256, "identity": 0.991, "score": 216.0, "evalue": 2.26e-69, "gene_start": 1, "gene_end": 113, "reference_start": 136, "reference_end": 248, "reference_sequence": "MPKDIIELPYNPISSDELKEIVAKKINESIDSKVQNQFSDYLEDRIDESSKLKIDINSEITNFLRNKINLNDAKKLIENYLNNKNKFEISNEYQINNEISKKIFNLLNTKGSSFDNYSSKKIFYVAYDHKTWIRVIVYTENNVKKISINPDVYILNQSNSYEQSKEILKKLNKQIFIERLNSIIDSLIENINEKDFNIKNFFEKNNDKKFFELFQKFSYNKYLIEAMQKINKEKDLIYRYSWETVDYE" }, "paralog": false, "identity": 0.9577464788732394, "score": 407.0, "evalue": 8.99e-147, "description": "Internal stop codon at 704390. Nonsense mutation around 704390.", "psc": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A5C6EDE", "UniRef:UniRef50_UPI000A5C6EDE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13857.609299999982, "isoelectric_point": 6.368831825256348 }, "id": "DFOKDJOJIG_1230", "locus": "GCA013367855_01229" }, { "type": "cds", "sequence": "contig_1", "start": 704736, "stop": 705041, "strand": "+", "frame": 3, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241181B1", "UniRef:UniRef90_UPI00241181B1" ], "nt": "ATGAATAAAAGAAATTTATTAAATTTTTTGTTTGTTCCCACTTTAATTCTTGCACCATTAACCGCTATATCGTGCGTTGATAAAAGTGAAAAAAGCGATTTGAATGCATTAGCAACTAAAATAGGGGATGTTGTAAATAAAAAAGAAAATCCTGCTATTGAAGTAACCAAAAAAATAATTAATGACTTATTGAAACAAGTATATAGGGATAATGATGTTGAAAAAATTAATTTTTTGGATCAACAACAAAATAAAGAATATCAAAAAAACATTTTTTTGAAAATTAAAGAACTTTCAAAAAAATGA", "aa": "MNKRNLLNFLFVPTLILAPLTAISCVDKSEKSDLNALATKIGDVVNKKENPAIEVTKKIINDLLKQVYRDNDVEKINFLDQQQNKEYQKNIFLKIKELSKK", "aa_hexdigest": "670d981eccc8d6508d2c5805232c3907", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 704736, "stop": 705344, "observations": { "mutation": [ 705039, 705111, 705162 ], "stop": [ 705039, 705111, 705162 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00241181B1", "query_cov": 1.0, "subject_cov": 0.4855769230769231, "identity": 1.0, "score": 185.0, "evalue": 1.13e-57, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MNKRNLLNFLFVPTLILAPLTAISCVDKSEKSDLNALATKIGDVVNKKENPAIEVTKKIINDLLKQVYRDNDVEKINFLDQQQNKEYQKNIFLKIKELSKKWNSKLNNTEKENIANQLNQIYEKNWYIVLTNINKFNLRFYEWLLFDKPEFLSQIRADGKHSKEYLNSLKSEKKPDNVPIINNYLRELKEGDESGELSNMNMLYLKKR" }, "paralog": false, "identity": 0.9851485148514851, "score": 389.0, "evalue": 1.93e-140, "description": "Internal stop codon at 705039, 705111, 705162. Nonsense mutation around 705039, 705111, 705162.", "psc": { "uniref90_id": "UniRef90_UPI00241181B1", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI00241181B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00241181B1", "UniRef:UniRef50_UPI00241181B1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00241181B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241181B1" ], "product": "aromatic motif membrane protein" } }, "id": "DFOKDJOJIG_1231", "locus": "GCA013367855_01230" }, { "type": "cds", "sequence": "contig_1", "start": 705168, "stop": 705431, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATAAGCCTGAATTTTTAAGCCAAATTAGGGCAGATGGAAAGCATAGCAAAGAATATTTAAATTCATTAAAAAGCGAAAAAAAACCAGATAATGTTCCGATTATTAATAATTATTTAAGGGAATTAAAAGAAGGCGATGAATCAGGAGAACTTTCAAATATGAACATGCTTTATTTAAAAAAAGATAGTATGATATTCAGATTTAGAGTTGTTGATACATTGACAAAACCAAAAATCAAACTAACTTGTTTTGTTTGA", "aa": "MFDKPEFLSQIRADGKHSKEYLNSLKSEKKPDNVPIINNYLRELKEGDESGELSNMNMLYLKKDSMIFRFRVVDTLTKPKIKLTCFV", "aa_hexdigest": "2a8313656ff3ae2836fdeefe9c741b3d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10215.78109999999, "isoelectric_point": 9.040159797668458 }, "id": "DFOKDJOJIG_1232", "locus": "GCA013367855_01231" }, { "type": "cds", "sequence": "contig_1", "start": 705465, "stop": 705629, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCAGATATTGTTCATAGCGCTTATATTCACGGCGTTCAAGAAGGATTCAATAGATTTGAAAATGATATGGCAAACCGTTATGGCAAACCTGCCAAAATGATCATGGGAATTTTCGATGATAATGCTTCTAGCGCCAACGGAGGGGAAGAAAATGAATAA", "aa": "MISDIVHSAYIHGVQEGFNRFENDMANRYGKPAKMIMGIFDDNASSANGGEENE", "aa_hexdigest": "b54393e84d362da2668720e0d9f5e886", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5979.497800000001, "isoelectric_point": 4.690774345397949 }, "id": "DFOKDJOJIG_1233", "locus": "GCA013367855_01232" }, { "type": "cds", "sequence": "contig_1", "start": 705622, "stop": 705780, "strand": "+", "frame": 1, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E405388", "UniRef:UniRef90_UPI001E405388" ], "nt": "ATGAATAAAAAAATTTTGATGACTCTATCATTAAGCACGTTAAGCATTATACCTAGCTGTTTTTCTTTAATTTCATGTACTAAAGAAAATAAACAAGATGAAAAAAAATCATTGAATCAAATAATTGAGGGCAACGAAAATGCAAACGAAAGAGAATGA", "aa": "MNKKILMTLSLSTLSIIPSCFSLISCTKENKQDEKKSLNQIIEGNENANERE", "aa_hexdigest": "b64cf36178635a37a8549461ed4726ef", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 705622, "stop": 705879, "observations": { "mutation": [ 705778 ], "stop": [ 705778 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E405388", "query_cov": 1.0, "subject_cov": 0.6046511627906976, "identity": 1.0, "score": 95.9, "evalue": 7.5e-25, "gene_start": 1, "gene_end": 52, "reference_start": 1, "reference_end": 52, "reference_sequence": "MNKKILMTLSLSTLSIIPSCFSLISCTKENKQDEKKSLNQIIEGNENANEREWKNFLNRDYVKEILRLSFDADVDAKNKYIAEQKN" }, "paralog": false, "identity": 0.9882352941176471, "score": 165.0, "evalue": 1.02e-54, "description": "Internal stop codon at 705778. Nonsense mutation around 705778.", "psc": { "uniref90_id": "UniRef90_UPI001E405388", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI001E405388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E405388", "UniRef:UniRef50_UPI001E405388" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E405388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E405388" ], "product": "aromatic motif membrane protein" } }, "id": "DFOKDJOJIG_1234", "locus": "GCA013367855_01233" }, { "type": "cds", "sequence": "contig_1", "start": 706096, "stop": 706302, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTGAATCATCAGATGAACAATCGGCGTTAACCGCGCAAGGTAATTCCTTGATTTTAGGCCATTTTTATAAACCAAAGAGTAATGAAATTATTCAATATACAACTCAAGTTACAAAAAATGACAAAAATGGTGAGAGAACTTATGATGTTTATTTATTAACAAAAGAAGGTTTTATTCTTCAATTAACTATTGATTGA", "aa": "MNKFESSDEQSALTAQGNSLILGHFYKPKSNEIIQYTTQVTKNDKNGERTYDVYLLTKEGFILQLTID", "aa_hexdigest": "a92758be7146349171454e00ea7f9761", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7789.6061, "isoelectric_point": 5.216478157043456 }, "id": "DFOKDJOJIG_1235", "locus": "GCA013367855_01234" }, { "type": "cds", "sequence": "contig_1", "start": 706857, "stop": 707024, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAATAATAGGAAGAACAACAAAAAAACAAAGGAAAATTATGTCAGAACAAAAACAACAAGCACAACCAGCAGTAGCTCCAACACAAGCAGAACCTGCTATTGTATCACGCGAAAAATTATTAGAAGCAGGAACATACTTTGGACACAGAGTAAGCCAATGA", "aa": "MLEIIGRTTKKQRKIMSEQKQQAQPAVAPTQAEPAIVSREKLLEAGTYFGHRVSQ", "aa_hexdigest": "7461acaa8e4de47d38c08816005aa396", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6180.082899999999, "isoelectric_point": 9.989134407043458 }, "id": "DFOKDJOJIG_1236", "locus": "GCA013367855_01235" }, { "type": "cds", "sequence": "contig_1", "start": 707061, "stop": 707162, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTCATATTATTGATATTGCCAAAACTCAAAAAACATTGGAATATGCTTACAAACTATTAAATAAAATGGCTCAAAAACCAATTTCATTTATTTGA", "aa": "MGIHIIDIAKTQKTLEYAYKLLNKMAQKPISFI", "aa_hexdigest": "82e2324bb86e16d48affdc20ce88ce1b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3821.5944, "isoelectric_point": 9.602904319763184 }, "id": "DFOKDJOJIG_1237", "locus": "GCA013367855_01236" }, { "type": "cds", "sequence": "contig_1", "start": 707361, "stop": 707801, "strand": "+", "frame": 3, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "COG:COG0052", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02967", "SO:0001217", "UniRef:UniRef50_D5E5E7", "UniRef:UniRef90_D1J8T8" ], "nt": "ATGGACAAAAAAATTGCCAAGTTGCAAAAGAACTTAGGCGGTATTAGAAAATTAGCAGGAAAAGCAGCAATGCCACAAGTAATGATTTGTGCATCACCTATTGATGATGAAATAGCAATTAGAGAAGCAAAAAAGAAAGGTTTGAAAATTTTTGCAATTCAAGATACAAACTCAAACCCAGATTTAGTTGACTTCGTAATACCAGCAAACGATGATTCTGTAAAGTCAATTACTTTAATTACAACTATCTTGGCAGACGCAATAGCATCAGCACATGGTGAAAAACCAGTATATGCATATAAGTCAAATGAAGAAATTATCTTGCCAGAAGAACAAAAACCAGAAAAGGTTGAAAAAATAGCAAACACTGAAGAAAGAAAACCAAGAACTTTTGTTAAAAAAGCTAACGTTGAATCAGAAAATACTAAGAAATCTGAATAA", "aa": "MDKKIAKLQKNLGGIRKLAGKAAMPQVMICASPIDDEIAIREAKKKGLKIFAIQDTNSNPDLVDFVIPANDDSVKSITLITTILADAIASAHGEKPVYAYKSNEEIILPEEQKPEKVEKIANTEERKPRTFVKKANVESENTKKSE", "aa_hexdigest": "3faafe1bfebc59bb844b5f86d14e5d0f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "rpsB" ], "truncated": "5-prime", "pseudogene": { "start": 707061, "stop": 707801, "observations": { "mutation": [ 707160, 707241 ], "stop": [ 707160, 707241 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8T8", "query_cov": 1.0, "subject_cov": 0.48344370860927155, "identity": 1.0, "score": 244.0, "evalue": 3.93e-79, "gene_start": 1, "gene_end": 146, "reference_start": 157, "reference_end": 302, "reference_sequence": "MSEQKQQAQPAQPAVAPTQAEPAIVSREKLLEAGTYFGHRVSQWNPKMKQYIWGKRMGIHIIDIAKTQKTLEYAYKLLNKMAQKPISFIWVGTKKQAKKAIESAAARTNSVYVSERWLGGTLTNSQTIFRSVRELERLEELQKSEYQGYTKKEGLLMDKKIAKLQKNLGGIRKLAGKAAMPQVMICASPIDDEIAIREAKKKGLKIFAIQDTNSNPDLVDFVIPANDDSVKSITLITTILADAIASAHGEKPVYAYKSNEEIILPEEQKPEKVEKIANTEERKPRTFVKKANVESENTKKSE" }, "paralog": false, "identity": 0.991869918699187, "score": 433.0, "evalue": 9.86e-156, "description": "Internal stop codon at 707160, 707241. Nonsense mutation around 707160, 707241.", "psc": { "uniref90_id": "UniRef90_D1J8T8", "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_D5E5E7", "cog_id": "COG0052", "cog_category": "J", "kegg_orthology_id": "K02967", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8T8", "UniRef:UniRef50_D5E5E7", "COG:COG0052", "COG:J", "KEGG:K02967", "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5E7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5E7" ], "product": "Small ribosomal subunit protein uS2" } }, "id": "DFOKDJOJIG_1238", "locus": "GCA013367855_01237" }, { "type": "cds", "sequence": "contig_1", "start": 707822, "stop": 707938, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGTAGATTTAAAAAAAATTAAGGAATTAAGAGAAAGAACAAATTCAGGATTTTTAGATGTTAAAAATGCATTAGAAGCAACAAACAACGATATTGAAAAAGCAATTGAATGA", "aa": "MSVDLKKIKELRERTNSGFLDVKNALEATNNDIEKAIE", "aa_hexdigest": "70bdda38816f371176ed85c51c179563", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4333.8732, "isoelectric_point": 5.289686775207519 }, "id": "DFOKDJOJIG_1239", "locus": "GCA013367855_01238" }, { "type": "cds", "sequence": "contig_1", "start": 707939, "stop": 708703, "strand": "+", "frame": 2, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0006414", "SO:0001217", "UniRef:UniRef50_Q98Q37", "UniRef:UniRef90_D1J8T9" ], "nt": "TTGCAAGAAAAAGGTATAGTTAAGGCTGCTAAAAAAGCCGGAAGAATTGCAGCAGAAGGAATAGTAAGAGCTGTTATTAAAGATAATATTGCTGTAATTTTTGAATTAAATTCAGAAACAGATTTTGTTGCTAAAAATCAATTATTTTTAGACTTTGCTAAATTATTAGAAGAAGAATTGCCAAAAGTTAATTTCTCAAATATCGATGATTTATTAGAACAAAAAATTAATGGTAAAACAATTGAAGAATATACAACAGATTTAACAGCAAAAATTGGCGAAAAGATTCATTTAAGAAGAGCATTAAAGTATGAAGCAAAAGAAGATGAAGTTGTTGCTGGATATACACATGCTAACAATAGAATTGCTACTATTTTAATCGCTAAAGGACATAACCAAGAAGCATTAAGACATGTTGCAATGCATATTGCAGCCTTAAACCCTGCTCATTTATTCGAAACATGTTTGGATGAAGAAGAATTAAAAGAAATTTATGAACGTATTAACAACGATCCTAAATTAGCATCTAAACCAGAAAAAATTCAAGTTTCTATGAAATCAGGAATGTTAAGAAAAGAATTCAATGAACGTGGCGTTTTAATGTATCAACCATTTGTTATGGAAGAATCAAAAACAGTTCAACAATATTTAACAGAAAACAAATTGCAATTAATAGATGCAACAAGATTTGAAGTTGGCGAAGGAATAGAAAAGAAAACTGTTGATTTTGCTACAGAAGTTGCTGAACAAATGTCACTTAAATAA", "aa": "MQEKGIVKAAKKAGRIAAEGIVRAVIKDNIAVIFELNSETDFVAKNQLFLDFAKLLEEELPKVNFSNIDDLLEQKINGKTIEEYTTDLTAKIGEKIHLRRALKYEAKEDEVVAGYTHANNRIATILIAKGHNQEALRHVAMHIAALNPAHLFETCLDEEELKEIYERINNDPKLASKPEKIQVSMKSGMLRKEFNERGVLMYQPFVMEESKTVQQYLTENKLQLIDATRFEVGEGIEKKTVDFATEVAEQMSLK", "aa_hexdigest": "3438d9ef9e9f6effb134627f2937a747", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8T9", "query_cov": 1.0, "subject_cov": 0.8668941979522184, "identity": 0.992, "score": 476.0, "evalue": 8.46e-169, "valid": true, "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_Q98Q37", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q37" ], "product": "Elongation factor Ts" }, "genes": [ "tsf" ], "id": "DFOKDJOJIG_1240", "locus": "GCA013367855_01239" }, { "type": "cds", "sequence": "contig_1", "start": 708848, "stop": 709222, "strand": "+", "frame": 2, "gene": null, "product": "Helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "nt": "ATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGTTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCGAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAGTTTTCCAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGA", "aa": "MNYNIHKYFHLSLEERFFIQRSLENNLSLRAISRQLNRSASTISREIKRNSNFNEYIFLRADKKSKFRAYHKYMFKFNINFEFEEFSKIFKSKYDKKYFGVKATMNYIEENFNVKKPTMRTVFN", "aa_hexdigest": "c57bd1123260a439ee4f69e485682c4a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0AAJ1UWR4", "query_cov": 1.0080645161290323, "subject_cov": 0.8116883116883117, "identity": 0.6, "score": 141.0, "evalue": 1.13e-40, "valid": false, "product": "Helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_A0AAJ1UWR4" }, "pscc": { "uniref50_id": "UniRef50_A0AAJ1UWR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "product": "Helix-turn-helix domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_1241", "locus": "GCA013367855_01240" }, { "type": "cds", "sequence": "contig_1", "start": 709406, "stop": 709687, "strand": "+", "frame": 2, "gene": null, "product": "IS30 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "nt": "TTGGTTCTTGGAAAAAGAGCAAATGGCTATAAAAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGCGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGA", "aa": "MVLGKRANGYKNILTLTERKTRVGFATFVSSKSPFEINSKIKELIKNKNLIVKTITIDNGIEFEKIGFLAKSLNIIIYRAEPYASFQRGSNEN", "aa_hexdigest": "151ece11318bfe9f9904904a28af0e1a", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001F20DEBE", "query_cov": 0.978494623655914, "subject_cov": 0.8272727272727273, "identity": 0.67, "score": 125.0, "evalue": 1.07e-35, "valid": false, "product": "IS30 family transposase", "uniref50_id": "UniRef50_UPI001F20DEBE", "go_ids": [ "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F20DEBE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "product": "IS30 family transposase" }, "genes": [], "id": "DFOKDJOJIG_1242", "locus": "GCA013367855_01241" }, { "type": "cds", "sequence": "contig_1", "start": 709694, "stop": 709804, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAGAAAAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGA", "aa": "MIRKEYQKGFNFNILTQEDLDTTIAKINNMPREILN", "aa_hexdigest": "36d3f6b1b3f8f640ff8ebfc59108e62d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4312.9214999999995, "isoelectric_point": 6.038597297668456 }, "id": "DFOKDJOJIG_1243", "locus": "GCA013367855_01242" }, { "type": "cds", "sequence": "contig_1", "start": 710014, "stop": 710841, "strand": "-", "frame": 1, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "RefSeq:WP_044730225.1", "SO:0001217", "UniParc:UPI0005CC9D11", "UniRef:UniRef100_UPI0005CC9D11", "UniRef:UniRef50_D1J8U0", "UniRef:UniRef90_D1J8U0" ], "nt": "ATGGAGAATAAATCTTTTAGTCAACTAGTAAAAGAAGAAATTATGTCTTTTCAGTTAAAAAAACAAGAAAAGTTTTTTTTGATGGCTGGCATAATTTATTGTTGTAATGTAAAAAACAATGAAGCTAAATTAGTAATAAATAATATTAAGATTAGGACATATATATTAAATATTTGTAATGAACTAAAAATTTCTTATACGTATACTAAAAAAAATGAGATCTCAATAAATACAAATTTTTTAGCTAACATAAGCGTTAAATATCAAGGAAAATTCTTTGCTGGTTTATTCCTTTCTTCGGGTTCGATAATAAATGGAAGAACAAATCACCTAGAGTTAAAATTTAATGATTTAATAAATGCAGAAAATGCTTTATCAATTTTAAATTCTCACGGATTAGAATTTAAAAAAATAATTAGAAAACAAAAAATAATAATTTATTTAAAAAAAATTGAAAATATTTGTGATTTTTTAAAAGCTATTGAAACCATTGAATCTTATTATTTATTCGAAGATGGCAAAATTCAAAGAGATTTTTATAATTCGGCAAATAGAATAACAAATCTCGACGTATATAATCAAGAACGTATTGCAAAAGCAAATTTAGAATTTATTAAAAATTATCAATTTATAAAAGAAAACAATCTCTTGGATGAGTTTAGCGTTGAAGAACTTACACTATTTGAATTAAAATTAAATAGTTTAGACAGTTCCTTGAATGAACTTAGTTTATTATTAGAAACAAAAAACATTAAAAAGTCTCGTTCTGCATTGAATCATTTCTTAATTAAGCTAAAAAAAATAACTAAAAAGTATCAAAAAAAATAG", "aa": "MENKSFSQLVKEEIMSFQLKKQEKFFLMAGIIYCCNVKNNEAKLVINNIKIRTYILNICNELKISYTYTKKNEISINTNFLANISVKYQGKFFAGLFLSSGSIINGRTNHLELKFNDLINAENALSILNSHGLEFKKIIRKQKIIIYLKKIENICDFLKAIETIESYYLFEDGKIQRDFYNSANRITNLDVYNQERIAKANLEFIKNYQFIKENNLLDEFSVEELTLFELKLNSLDSSLNELSLLLETKNIKKSRSALNHFLIKLKKITKKYQKK", "aa_hexdigest": "bf99c3d455573e7739357d0ed10cfe2c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0005CC9D11", "ncbi_nrp_id": "WP_044730225.1", "uniref100_id": "UniRef100_UPI0005CC9D11", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005CC9D11", "RefSeq:WP_044730225.1", "UniRef:UniRef100_UPI0005CC9D11" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005CC9D11", "uniref90_id": "UniRef90_D1J8U0" }, "psc": { "uniref90_id": "UniRef90_D1J8U0", "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_D1J8U0", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U0" ], "product": "DNA-binding protein WhiA" }, "genes": [ "whiA" ], "id": "DFOKDJOJIG_1244", "locus": "GCA013367855_01243" }, { "type": "cds", "sequence": "contig_1", "start": 710890, "stop": 711417, "strand": "-", "frame": 1, "gene": "pncA", "product": "Isochorismatase-like domain-containing protein", "db_xrefs": [ "COG:COG1335", "COG:HR", "RefSeq:WP_047207792.1", "SO:0001217", "UniParc:UPI0006402F73", "UniRef:UniRef100_A0A6A8PYM3", "UniRef:UniRef50_A0A077L748", "UniRef:UniRef90_D1J8U1" ], "nt": "ATGAAAGCAATTTTTGTTGTTGATATGGTTAATGGTTTTTGCAAAGAAGGGGCACTATATAGTGACAATATTGAGTCAATAATTGTTCCTATAAAAAATTTTTTAGAAACTCAATATAAAGACAATGATATATATTTTTTAAATGATGCTCATAGTAGCGATGATATTGAAATGCAATCATATCCAATACATTGCTTAAAAAATAGTAAAGAAAGCCAAGTAGTAGATGAATTAAAAAAATATGCTAAAAATATAATTGAAAAGAATTCTACTAACTCCTTTTTTGCATTAAAAAAGGAAATTTTAAGCAAGTATGATTCCTTTGAAATTATAGGTTGTTGTTCAGATATTTGCATTTTACAATTTGCAATAACTCTAAAAACTTATTTCAATCATTTAAAGCAAAATAAAGAAATTATTGTCTTTAAAAATTTAATTGCAACATTTAATATTCCAAATCATAATTCTCAAGAATATCACGATTTTGCTTTAAATTTAATGGCTAATGCAGGCATAAAAATTAAATAA", "aa": "MKAIFVVDMVNGFCKEGALYSDNIESIIVPIKNFLETQYKDNDIYFLNDAHSSDDIEMQSYPIHCLKNSKESQVVDELKKYAKNIIEKNSTNSFFALKKEILSKYDSFEIIGCCSDICILQFAITLKTYFNHLKQNKEIIVFKNLIATFNIPNHNSQEYHDFALNLMANAGIKIK", "aa_hexdigest": "eaef33a7d4e7dbefea15191adee023bb", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0006402F73", "ncbi_nrp_id": "WP_047207792.1", "uniref100_id": "UniRef100_A0A6A8PYM3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0006402F73", "RefSeq:WP_047207792.1", "UniRef:UniRef100_A0A6A8PYM3" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PYM3", "uniref90_id": "UniRef90_D1J8U1" }, "psc": { "uniref90_id": "UniRef90_D1J8U1", "gene": "PncA", "product": "Isochorismatase-like domain-containing protein", "uniref50_id": "UniRef50_A0A077L748", "cog_id": "COG1335", "cog_category": "HR" }, "pscc": { "uniref50_id": "UniRef50_A0A077L748", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L748" ], "product": "Isochorismatase-like domain-containing protein" }, "genes": [ "pncA" ], "id": "DFOKDJOJIG_1245", "locus": "GCA013367855_01244" }, { "type": "cds", "sequence": "contig_1", "start": 711589, "stop": 711741, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCGAAGAACAATTAGTTGTTACTTCAAGTCCTAAGAAAAAGATTGGCTTCTTTTCAGCTATTCTTATCGTTATGGGTTCGGCTATCGGTGTTGGGGTTTTCCTACGTTCAAAGAACGTTTTACAAAACTCAGCTAACAACGTCGTATGA", "aa": "MAEEQLVVTSSPKKKIGFFSAILIVMGSAIGVGVFLRSKNVLQNSANNVV", "aa_hexdigest": "02596721837798f5e42380334bb00f3d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5293.2089000000005, "isoelectric_point": 9.996161460876465 }, "id": "DFOKDJOJIG_1246", "locus": "GCA013367855_01245" }, { "type": "cds", "sequence": "contig_1", "start": 711895, "stop": 712026, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTATCTATATCTACCATTTACTTACTTCTTTATGCCTTACTACGTTATCGTTCAATTCCAAGACGGTATCGCAGGATTCGTAGGATACAGCAAAGTAGGATTTGGTGGTTCTACAAGTGCTCCATGA", "aa": "MTYLYLPFTYFFMPYYVIVQFQDGIAGFVGYSKVGFGGSTSAP", "aa_hexdigest": "bae965101d051791fb40e27a7bc2cb4e", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4831.514600000001, "isoelectric_point": 5.586102104187011 }, "id": "DFOKDJOJIG_1247", "locus": "GCA013367855_01246" }, { "type": "cds", "sequence": "contig_1", "start": 712387, "stop": 712542, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACACCCAGAAAAAACCCCTGCTGCTATTGTTATTGGTTTAGCATCAATGACTGTTCTATACATGTTAATTGCTGTTACAATGACATTAGGTGGAAACAACGGTTCATTCTACGGATTTGGAGATGAATTAAACGCTGTTAAAGCAGGTTGA", "aa": "MKHPEKTPAAIVIGLASMTVLYMLIAVTMTLGGNNGSFYGFGDELNAVKAG", "aa_hexdigest": "9b2780453f473bbd2db75d02ed91c875", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5301.184600000001, "isoelectric_point": 6.514055442810059 }, "id": "DFOKDJOJIG_1248", "locus": "GCA013367855_01247" }, { "type": "cds", "sequence": "contig_1", "start": 712744, "stop": 712857, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATCTTCACTGCTATTGGTGCTTACGCATACTTTGGTGGAGGATATTCAACAGACTACGGTTACCAAATTGATAACCTATTAACATTTGCTGACTTGATGGCAAACTGA", "aa": "MVIFTAIGAYAYFGGGYSTDYGYQIDNLLTFADLMAN", "aa_hexdigest": "80ebdad5c03bf7b06982cc073cecfbe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4048.5029000000013, "isoelectric_point": 4.0500284194946286 }, "id": "DFOKDJOJIG_1249", "locus": "GCA013367855_01248" }, { "type": "cds", "sequence": "contig_1", "start": 712999, "stop": 713244, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTCTTAGCAATTGACCCATTCATATCAATAGGTCTATCAGCAGCTCAATACGTTGCAGATAAAACAGAAGACAACAGAAACGGCTTAGTAGGAAACATTGCTACATCAGTTCTATTTATAATATATGCAGCAGTTTCATTCTTAGGCGCTCCAGTTGAAAGACAAATTGCTAAATCTAACAAGATTAAATTTGATAGAGTTCTTTCAGGAAATATTTGTGCACACGAAGTTCCAGAATGA", "aa": "MFFLAIDPFISIGLSAAQYVADKTEDNRNGLVGNIATSVLFIIYAAVSFLGAPVERQIAKSNKIKFDRVLSGNICAHEVPE", "aa_hexdigest": "44145aa96bdbb9c0c1e865904b400d15", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8787.039699999998, "isoelectric_point": 5.7058618545532225 }, "id": "DFOKDJOJIG_1250", "locus": "GCA013367855_01249" }, { "type": "cds", "sequence": "contig_1", "start": 713446, "stop": 713712, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "ATGAAAAATAGTAAAGAACAGATTAACAATATTCCTTATGTTTTAGATGAAACATTGATTAACACAAATTTAAAAACCGGCCTAAGTTCGCAAGAGGCCGCCTTAAGACTTAACAAGTTTGGAAAAAATGAGTTGCATAAAGCAAAAAAAATAAATCCATTTTTAATATATTTAGAACAATTTAAAGATATATTGGTCATTATATTATTATTTGCAGCAATAATATCTTATGCTCTTGCGATTGTTTCAGGAACTCAAAATAATTGA", "aa": "MKNSKEQINNIPYVLDETLINTNLKTGLSSQEAALRLNKFGKNELHKAKKINPFLIYLEQFKDILVIILLFAAIISYALAIVSGTQNN", "aa_hexdigest": "8848288d05769d8761528e5a8c166d86", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 56, "aa_cov": 0.6363636363636364, "hmm_cov": 1.0, "evalue": 4.435410284075625e-21, "score": 70.01466369628906, "start": 21, "stop": 76 } ], "seq_stats": { "molecular_weight": 9919.49649999999, "isoelectric_point": 9.30196590423584 }, "id": "DFOKDJOJIG_1251", "locus": "GCA013367855_01250" }, { "type": "cds", "sequence": "contig_1", "start": 713842, "stop": 714351, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCAAATTAAGTCCCTTACAAGCTAAAGTCGTCCGTGATGGCAATTTAATAACATTAGCATCAAGTGATATAACCGTTGGCGATATTGTTTTAATTGAATCAGGTGATGTTGTACCTTCTGATGGTTATTTGTTGTTTAGTTCAAATTTAACTGTTATTGAGAGTGCATTAACAGGAGAAAGTGAACCGGCGACAAAAGATTACCAAGCTAAAAGAAATTTAACATTAGCAATGGCGGATAGACAATTTATGGTTTATTCTTCGACTGTGGTTGCTACAGGAACAGCTTATTTTGTTGTTACATCAGTAGGTGAAAATAACGAAATCGGAAAAATTTCTAAATTAGTTAATCAACAAAAAGAATCAATGACTCCTCTACAAATTAAAATCAATAAATTAGGAAAAATATTTGGATATTCAGGTCTTGCATTATTTTTAGCAAGTTTAGTGACTCAAATAGTTTTCCATGCTGTATCAAGAAATTCTTTTGCTGATACAATGTTTTGA", "aa": "MSKLSPLQAKVVRDGNLITLASSDITVGDIVLIESGDVVPSDGYLLFSSNLTVIESALTGESEPATKDYQAKRNLTLAMADRQFMVYSSTVVATGTAYFVVTSVGENNEIGKISKLVNQQKESMTPLQIKINKLGKIFGYSGLALFLASLVTQIVFHAVSRNSFADTMF", "aa_hexdigest": "dc8671917c66c2e4a8f271826dce9f44", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18220.733799999995, "isoelectric_point": 5.791972923278809 }, "id": "DFOKDJOJIG_1252", "locus": "GCA013367855_01251" }, { "type": "cds", "sequence": "contig_1", "start": 714451, "stop": 714861, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAGAAAGAATGCGATTGTTAAGAACTTAATGGCTGTTGAGTCTCTTGGCAATTGTGCAGTAATATGTTCAGATAAAACAGGAACATTAACACAAAACAAGATGACAATTGTAGATGCTTTTGATGGAAGTCAACTACTATCAAATAATAGTGAAATTAAAAACTTTTTAAATATTATAGAATTAGGTTCGTTATGTTGCGATGCAAACTTGACTCGCAATGAAAAAGATGAATATGTTGTAGTTGGAGATCCAACAGAAATAGCATTTTTATATGAACTTGAAAAATTTTCTAATTTTAAATATAAAAACAACCTCGAAATAGAATATAAGCGTTTGCATTTGTTCCCGTTTGATTCAGATAGAAAAATGAAATCAGTAATAAATCAAAACGACAACAATTATTGA", "aa": "MARKNAIVKNLMAVESLGNCAVICSDKTGTLTQNKMTIVDAFDGSQLLSNNSEIKNFLNIIELGSLCCDANLTRNEKDEYVVVGDPTEIAFLYELEKFSNFKYKNNLEIEYKRLHLFPFDSDRKMKSVINQNDNNY", "aa_hexdigest": "2d7b5a1415aa3084a2c47abec1a2062d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15515.485699999983, "isoelectric_point": 5.193060493469236 }, "id": "DFOKDJOJIG_1253", "locus": "GCA013367855_01252" }, { "type": "cds", "sequence": "contig_1", "start": 714871, "stop": 715362, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGGGCGCTCCTGAAATAATAATTGAAAAATGTAATTTTGATTCACCTTCATTAAAAGAAAAATATTTACAAATAAATAATCAATGGGCAAATAATGCTTATAGAGTCTTAGCAATAGCAAGAAAATCCTTAAAAAAGGAAACTATTGAGAAATTCAATGAATCATCAATTTCTAAATTACAAGAGCATGTAGAAAATGATTTAGAAATTGTTGGATTAGTTGCAATGATTGATCCTCCAAGAGAAAGTGCAAAGGATGCTATTACATTATGTAAACAAGCGGGAATAAAACCTGTTATGATTACTGGCGATAATTTAAATACAGCAAAGGCAATAGCAAAAAATCTTGGTATATATAGTGATCAAGACCGCGCTATAACAGGAACTGAACTTTCAAGCATTTCTGACGAAGATTTAGTTCAAAATATTGAAAAATACAGTGTTTATGCTAGAGTTAAACCAGAAGATAAATTAAGAATTGTTAATGCTTGA", "aa": "MGAPEIIIEKCNFDSPSLKEKYLQINNQWANNAYRVLAIARKSLKKETIEKFNESSISKLQEHVENDLEIVGLVAMIDPPRESAKDAITLCKQAGIKPVMITGDNLNTAKAIAKNLGIYSDQDRAITGTELSSISDEDLVQNIEKYSVYARVKPEDKLRIVNA", "aa_hexdigest": "7aea35737918b01457ad032788290f10", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 150, "aa_cov": 0.8220858895705522, "hmm_cov": 0.7933333333333333, "evalue": 8.993086634209775e-13, "score": 44.670021057128906, "start": 28, "stop": 161 } ], "seq_stats": { "molecular_weight": 18178.582399999992, "isoelectric_point": 5.561775016784667 }, "id": "DFOKDJOJIG_1254", "locus": "GCA013367855_01253" }, { "type": "cds", "sequence": "contig_1", "start": 715387, "stop": 715665, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGGTGATGGTGTTAATGATGCTCCAGCATTAAAAGCTTCAGATATAGGCTGCGCTATGGGTATAACAGGAACTGAAGCTTCTAAGCAGGCTGCAAATGTTATATTGGCAGATGATAATTTTGCCACCATAGTTTCAGCAGTAGCAAACGGAAGATCAATTTATCAAAAAATTAAAAACGTAATTCAAAGTTTATTGATTACATCGATTGCAGAGATCATTTTACTATTTATAGGTTTGTTTTTATTTAAAGCGATTTTTTCAAGTGCTGAATGA", "aa": "MTGDGVNDAPALKASDIGCAMGITGTEASKQAANVILADDNFATIVSAVANGRSIYQKIKNVIQSLLITSIAEIILLFIGLFLFKAIFSSAE", "aa_hexdigest": "d5e62797fa58ea977c0fde073636f9ca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9562.986599999993, "isoelectric_point": 4.842193412780761 }, "id": "DFOKDJOJIG_1255", "locus": "GCA013367855_01254" }, { "type": "cds", "sequence": "contig_1", "start": 716101, "stop": 716346, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGTAGTATTTGTAAAAAATATAAATCTAGTGTTTGGATGTTACACCGAAATTCATTCTAAGCCCGAACTTTTGGCACTAGGATTAGCATTACCATTAGTAATTATTCCAATATATTCAATTCATAAATTTATCATATATTTAATTGAAAAAAACAAAATTAAAGAAAATAAAATTACAGAATTTAAAATGATATTACCTCCAAGGCAAGTCAAAAGACATCAAAAAAATGTAGATCTATAG", "aa": "MIVVFVKNINLVFGCYTEIHSKPELLALGLALPLVIIPIYSIHKFIIYLIEKNKIKENKITEFKMILPPRQVKRHQKNVDL", "aa_hexdigest": "c74748662da2ee95ba1139799a9053f2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9469.444699999991, "isoelectric_point": 9.673884010314943 }, "id": "DFOKDJOJIG_1256", "locus": "GCA013367855_01255" }, { "type": "cds", "sequence": "contig_1", "start": 716412, "stop": 716777, "strand": "-", "frame": 2, "gene": null, "product": "Zinc-ribbon domain-containing protein", "db_xrefs": [ "RefSeq:WP_020002519.1", "SO:0001217", "UniParc:UPI0003612D14", "UniRef:UniRef100_UPI0003612D14", "UniRef:UniRef50_D1J8U5", "UniRef:UniRef90_D1J8U5" ], "nt": "ATGAATTATTGTCCAAATTGTGGAACTAAATTAAATAACGCTAATTATTGCCCAAATTGCGGAAAAAAGGTGGAAAAAGATGCATATGTAACAACACAAACGAATACAACGCAACCATCTCAACCTTATTTGCAACGTCAACAACAAATCCAAAATTCACCAACTAATGGATTTGCTATTGCCGGATTTATATTGTCATTTTTCTTTGCAATATTAGGTCTTATTTTTAGCTGTATTGGGCTTAGCAAGTCAAAATCAATGAATGATTCAGGAAGAACTTTGTCAATAGCAGGAATTATTATTTCTTGCTTAACTATGTTTTTTGCCTTTGTCATATTCATTACCGTAATGAGCGTAAGCCTTTAA", "aa": "MNYCPNCGTKLNNANYCPNCGKKVEKDAYVTTQTNTTQPSQPYLQRQQQIQNSPTNGFAIAGFILSFFFAILGLIFSCIGLSKSKSMNDSGRTLSIAGIIISCLTMFFAFVIFITVMSVSL", "aa_hexdigest": "ce78ce91570de61f70da06975e6fe1a3", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0003612D14", "ncbi_nrp_id": "WP_020002519.1", "uniref100_id": "UniRef100_UPI0003612D14", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003612D14", "RefSeq:WP_020002519.1", "UniRef:UniRef100_UPI0003612D14" ] }, "ips": { "uniref100_id": "UniRef100_UPI0003612D14", "uniref90_id": "UniRef90_D1J8U5" }, "psc": { "uniref90_id": "UniRef90_D1J8U5", "product": "Zinc-ribbon domain-containing protein", "uniref50_id": "UniRef50_D1J8U5" }, "pscc": { "uniref50_id": "UniRef50_D1J8U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U5" ], "product": "Zinc-ribbon domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_1257", "locus": "GCA013367855_01256" }, { "type": "cds", "sequence": "contig_1", "start": 716895, "stop": 716987, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATGTAAGGAAATGCACTTTATGCAACTTGACGGTGAAACATTTGATATAGAAAATAAAATATATATAAACAAAAAAATAATTAATTAA", "aa": "MECKEMHFMQLDGETFDIENKIYINKKIIN", "aa_hexdigest": "5ceec8e9950070a511ed41a7c5f6846c", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3676.2641000000012, "isoelectric_point": 5.00463924407959 }, "id": "DFOKDJOJIG_1258", "locus": "GCA013367855_01257" }, { "type": "cds", "sequence": "contig_1", "start": 717189, "stop": 717779, "strand": "-", "frame": 2, "gene": "lCB5", "product": "Transcriptional regulator", "db_xrefs": [ "COG:COG1597", "COG:IR", "SO:0001217", "UniRef:UniRef50_D1J8U6", "UniRef:UniRef90_D1J8U6" ], "nt": "ATGCTATATATATTTTATAATTCTCTATCAAAGAGTGGAAAATGTCAAAGAAAGATGTATGACATAGTTTCTGACGCCATTAAAGTATTTTTACAACCTGAGGTAAAAATATTTGACATTATAAAAATTAAAGACAGTCAAAATCTAGTTTCTCAAATGGATATAGAAAAAGATATTGTTTTATTAATTGGCGGTGATGGAACATTAACCAATATCACCAATAAAATTCACAATATAAAACCTTTACCCAAAATTTATGCATATAAAGCAGGAACGGGCAACGATTTTCTAAGGTCTTTAAAAATAAAACAAGATGTTCAAATAATACATAAAAAATATTATTTGATATCTCCTTACATTACCAACTTACCAACAATTAAATTTAAAAACGACCAAGATGAAAGATCTTTTTTAAATGGCATTGGTTTTGGTATTGATGCAGTTATTGCAAAGAAAGTTAATGATGAAAAAATGCTTAAAAATAAATCGTCATTTGCTAAAGTAACAATAAATGCATTTAAGACATTCAAAACAATTCCTTTAATAAAAATTAATGTAGATGGAAAAGAATTAGAATTTAAAAATGTTTGA", "aa": "MLYIFYNSLSKSGKCQRKMYDIVSDAIKVFLQPEVKIFDIIKIKDSQNLVSQMDIEKDIVLLIGGDGTLTNITNKIHNIKPLPKIYAYKAGTGNDFLRSLKIKQDVQIIHKKYYLISPYITNLPTIKFKNDQDERSFLNGIGFGIDAVIAKKVNDEKMLKNKSSFAKVTINAFKTFKTIPLIKINVDGKELEFKNV", "aa_hexdigest": "4d9360ec907b86c58c4ad92e3c18d8ba", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "lCB5" ], "truncated": "5-prime", "pseudogene": { "start": 716887, "stop": 717779, "observations": { "deletion": [ 716909 ], "mutation": [ 717068, 717080, 717191 ], "stop": [ 716900, 717068, 717080, 717191 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8U6", "query_cov": 1.0, "subject_cov": 0.6068111455108359, "identity": 0.995, "score": 383.0, "evalue": 1.17e-132, "gene_start": 1, "gene_end": 196, "reference_start": 1, "reference_end": 196, "reference_sequence": "MLYIFYNSLSKSGKCQRKMYDIVSDAIKVFLQPEVKIFDIIKIKDSQNLVSQMDIEKDIVLLIGGDGTLTNITNKIHNIKPLPKIYAYKAGTGNDFLRSLKIKQDVQIIHKKYYLISPYIANLPTIKFKNDQDERSFLNGIGFGIDAVIAKKVNDEKMLKNKSSFAKVTINAFKTFKTIPLIKINVDGKELEFKNVWLASIMNNCYFGGGMKLAPNCNNTDKLNLIIINNVSKWKLAWLFFSVYSGKHLRYKKYVHEIQGYKFSMECKEMHFMQLDGETFDIENKIYINKKKNQLNKKRPNKNWDVFLLLYCVIFIYKINLKK" }, "paralog": false, "identity": 0.9797979797979798, "score": 572.0, "evalue": 2.4e-209, "description": "Internal stop codon at 716900, 717068, 717080, 717191. Frameshift due to deletion around 716909. Nonsense mutation around 717068, 717080, 717191.", "psc": { "uniref90_id": "UniRef90_D1J8U6", "gene": "LCB5", "product": "Transcriptional regulator", "uniref50_id": "UniRef50_D1J8U6", "cog_id": "COG1597", "cog_category": "IR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8U6", "UniRef:UniRef50_D1J8U6", "COG:COG1597", "COG:IR" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U6" ], "product": "Transcriptional regulator" } }, "id": "DFOKDJOJIG_1259", "locus": "GCA013367855_01258" }, { "type": "cds", "sequence": "contig_1", "start": 717783, "stop": 718529, "strand": "-", "frame": 2, "gene": "argE", "product": "Xaa-His dipeptidase", "db_xrefs": [ "COG:COG0624", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZY24", "UniRef:UniRef90_D1J8U7" ], "nt": "ATGATCTATGTAACAGAAATTCATTTTCCAAAGGTTCCAGGAGTTGAATTAAATGGGGGCGACGTTGTTAACCAAATTCCTGATTCAACATCTTTAGTATTGAATGAAAAACCTAATATTATTAAAGGAATTGGTGGACACGGCTCAACACCTGAAAAAGGTGACAATGCAATAATTAAAGCCATTAATATAGCAAGCAAAGAAAATAAAGAATTTGCTAAATCTAAATTATTTAAATTTATTAAAGAAAATTTAAATAGCAAATCATTTAATTTAAGTAATATTTTTAAAAACTATAGTGATTTTTCGGGCGAATTATCTGCAAATTTAGGCATTATTAGAACCTTGAAAAATGAATATGTTTTAACTTTTGATTTTAGAGTCCCAGTTTCTCATAAAATTGATGATGTAACTAAAAGCTTAAATGATTATTTAAAAGATGAATTTGGCGATGATGTATTTTCAAAATTGCAAGGATTCAAGGAACCAAAGTATATTGAACAAGATTCTCCAATTGTAAGAATTCTTATGGATACATATAATGAAGCGATGAATTCAAATGAAAAACCTTTAGCAATTGGTGGCGGTACATTTGCAAGATTAATAAAAACCTGTGTTGCTTTTGGATCAACTAAATATATGCATTTAATGCATGGACCAAACGAATATTTTACATTTGATGAAATAAAATCATCACTTGAAATTTATATCAATGCATTAAATAGATTACAAGATTTGGAAAAATAA", "aa": "MIYVTEIHFPKVPGVELNGGDVVNQIPDSTSLVLNEKPNIIKGIGGHGSTPEKGDNAIIKAINIASKENKEFAKSKLFKFIKENLNSKSFNLSNIFKNYSDFSGELSANLGIIRTLKNEYVLTFDFRVPVSHKIDDVTKSLNDYLKDEFGDDVFSKLQGFKEPKYIEQDSPIVRILMDTYNEAMNSNEKPLAIGGGTFARLIKTCVAFGSTKYMHLMHGPNEYFTFDEIKSSLEIYINALNRLQDLEK", "aa_hexdigest": "5a54aa0d90e982c4bb7cec90702eb2d2", "start_type": "ATG", "rbs_motif": null, "genes": [ "argE" ], "truncated": "3-prime", "pseudogene": { "start": 717783, "stop": 718829, "observations": { "mutation": [ 718553, 718664, 718802 ], "stop": [ 718553, 718664, 718802 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8U7", "query_cov": 1.0, "subject_cov": 0.5662100456621004, "identity": 0.992, "score": 485.0, "evalue": 3.62e-170, "gene_start": 1, "gene_end": 248, "reference_start": 191, "reference_end": 438, "reference_sequence": "MKKFITYKQSKEDFDEMIKNIANICAIPSISEEDFSSEFPFGKETDNALNYALNLAKSFGFNIYKDPKNRYGFAEFGNGDKIIGILAHLDVVPAGDESQWRTSAFVPVITNESIIGRGSLDDKGPAIINLYAMKYIHDHGLLSNEWKIRIIFGISEETTMKSMKSYLADFKDPYISYTPDGEWPLIYAEKMIYVTEIHFPKVPGVELNGGDVVNQIPDSTSLVLNEKPNIIKGIGGHGSTPEKGDNAIIKAINIASKENKEFAKSKLFKFIKENLNSKSFNLSNIFKNYSDFSGELSANLGIIRTLKNEYVLTFDFRVPVSHKIDDVTKSLNDYLKDEFGNDVFSKLQGFKEPKYIEQDSPIVRILMDTYNEAMNSNEKPLAIGGGTFARLIKTCVAFGSTKYMHLMHGPNEYFTFDEIKSSLEIYINALNRLQDLEE" }, "paralog": false, "identity": 0.985632183908046, "score": 681.0, "evalue": 7.43e-250, "description": "Internal stop codon at 718553, 718664, 718802. Nonsense mutation around 718553, 718664, 718802.", "psc": { "uniref90_id": "UniRef90_D1J8U7", "gene": "ArgE", "product": "Xaa-His dipeptidase", "uniref50_id": "UniRef50_A0A448ZY24", "cog_id": "COG0624", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8U7", "UniRef:UniRef50_A0A448ZY24", "COG:COG0624", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZY24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZY24" ], "product": "Dipeptidase SA1572" } }, "id": "DFOKDJOJIG_1260", "locus": "GCA013367855_01259" }, { "type": "cds", "sequence": "contig_1", "start": 718800, "stop": 719099, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTTATTACTTATAAGCAAAGTAAAGAAGACTTTGATGAAATGATTAAAAATATTGCAAACATTTGTGCAATTCCTTCGATTAGTGAAGAAGATTTTAGTAGTGAATTTCCTTTTGGAAAAGAAACTGACAATGCTTTAAATTATGCATTAAATCTTGCCAAAAGTTTTGGATTTAATATTTATAAAGATCCTAAAAATCGTTATGGGTTTGCTGAATTTGGCAATGGAGATAAGATTATTGGAATACTGGCCCACCTTGACGTTGTTCCTGCAGGAGATGAAAGCCAATGA", "aa": "MKKFITYKQSKEDFDEMIKNIANICAIPSISEEDFSSEFPFGKETDNALNYALNLAKSFGFNIYKDPKNRYGFAEFGNGDKIIGILAHLDVVPAGDESQ", "aa_hexdigest": "6d5702e55358a66b66de891d7d2c573f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11122.363599999988, "isoelectric_point": 4.749943733215332 }, "id": "DFOKDJOJIG_1261", "locus": "GCA013367855_01260" }, { "type": "cds", "sequence": "contig_1", "start": 719350, "stop": 719589, "strand": "-", "frame": 1, "gene": null, "product": "endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00068CCCF8", "UniRef:UniRef90_UPI00068CCCF8" ], "nt": "TTGCAAGGCAATAAAATATCCAAAAATGGTACAAAAACTGGTTCTTCAGTTGAAGATGGTGGACCTGTTACCGAACCTATTAATGAATTTAAAGGTGATGTTGCTAGGGCATATTTATACTTTTGTTTAACTTATAACGATTTAAATATAACATCAAATAATCAATCAACTCGTGTATTTAAAGAAATTAATAGAAAAAATACTATAACTCCTATATTTTTAAAAACATTTTTAGCTTGA", "aa": "MQGNKISKNGTKTGSSVEDGGPVTEPINEFKGDVARAYLYFCLTYNDLNITSNNQSTRVFKEINRKNTITPIFLKTFLA", "aa_hexdigest": "9f1f7b85af4c34dd1ee2b3a750279424", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 719176, "stop": 719657, "observations": { "deletion": [ 719642 ], "stop": [ 719351 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00068CCCF8", "query_cov": 1.0, "subject_cov": 0.49375, "identity": 0.975, "score": 155.0, "evalue": 3.68e-47, "gene_start": 1, "gene_end": 79, "reference_start": 24, "reference_end": 102, "reference_sequence": "MFDQPDKLVNAWHANYPYGTVKELQGNKISKNGTKTGLSVEDGGPVTEPINEFKGDVARAYLYFCLTYNDLNITSNNQSTRVFKEINRKNTITPIFLKTFLAWNNLDKVSKFDIDRNNGVYKHQGNRNPFIDYPKLIDVVYNNNNDYVFKNKGIAVGLNK" }, "paralog": false, "identity": 0.975, "score": 316.0, "evalue": 6.36e-113, "description": "Internal stop codon at 719351. Frameshift due to deletion around 719642.", "psc": { "uniref90_id": "UniRef90_UPI00068CCCF8", "product": "endonuclease", "uniref50_id": "UniRef50_UPI00068CCCF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00068CCCF8", "UniRef:UniRef50_UPI00068CCCF8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00068CCCF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00068CCCF8" ], "product": "endonuclease" } }, "id": "DFOKDJOJIG_1262", "locus": "GCA013367855_01261" }, { "type": "cds", "sequence": "contig_1", "start": 719770, "stop": 720252, "strand": "-", "frame": 1, "gene": null, "product": "endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A5CC56", "UniRef:UniRef90_UPI0034A5CC56" ], "nt": "ATGAAAAAATCCAAAAAAATATTCCTAACTATGGGTTCTATTTTACCTTTGCTAATTTCAACACCATTAGTTGCTGCTGGATGTGTAAAACCTGAAGCTAAACCAGAAGGTGAAAAACCAAAAACAACACCAGAAAAGAGCCCTGAAGCTAAACTAGAAGGTGAAAAACCCAAAACAACCCCCGAAAAGAGCCCTGAAGCTAAACCAGAAAAACCTAAGATGCCTGAAGCTAAACCCTTAGATAATAAGATAATTTACGATAATTCAAATGACTTTTATAAATCCTTAGATGGCTTAAGTGGACAAAACCTAAGAGATAACTTATTTGCATTGCAAAAAGCTCATAGAAATCACTCAACAAAATATAATGACTTATTTGATACATATAAAGAAGCTTTTGTTGATAAATATTATGAAAAAGATGGATCTGTTTTAGATATCTATGAAGAAATCCCAGATAAAGAAGATGTTTTTACTAATTGA", "aa": "MKKSKKIFLTMGSILPLLISTPLVAAGCVKPEAKPEGEKPKTTPEKSPEAKLEGEKPKTTPEKSPEAKPEKPKMPEAKPLDNKIIYDNSNDFYKSLDGLSGQNLRDNLFALQKAHRNHSTKYNDLFDTYKEAFVDKYYEKDGSVLDIYEEIPDKEDVFTN", "aa_hexdigest": "96fa63cc0c69c6f8355747d0c56844d5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 719641, "stop": 720252, "observations": { "mutation": [ 719652, 719697, 719772 ], "stop": [ 719652, 719697, 719772 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A5CC56", "query_cov": 1.0, "subject_cov": 0.7843137254901961, "identity": 0.95, "score": 251.0, "evalue": 5.28e-83, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MKKSKKIFLTMGAILPLLISTPLVAAGCVKPEAKPESEKPGTTPEKKPEVKPEGEKPKTTPEKSPEAKPEKPKMPEAKSLDNKIIYDNSNDFYKSLDGLSGQNLRDNLFALQKAHRNHSTKYNDLFDTYKDAFVDKYYEKDGSVLDIYEEIPDKEDVFTNWHGKYRDTGKREGQGMNREHLVPQSWFRKSAPMRTDAHHVWPTG" }, "paralog": false, "identity": 0.9359605911330049, "score": 339.0, "evalue": 6.17e-120, "description": "Internal stop codon at 719652, 719697, 719772. Nonsense mutation around 719652, 719697, 719772.", "psc": { "uniref90_id": "UniRef90_UPI0034A5CC56", "product": "endonuclease", "uniref50_id": "UniRef50_UPI0034A5CC56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A5CC56", "UniRef:UniRef50_UPI0034A5CC56" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A5CC56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A5CC56" ], "product": "endonuclease" } }, "id": "DFOKDJOJIG_1263", "locus": "GCA013367855_01262" }, { "type": "cds", "sequence": "contig_1", "start": 720409, "stop": 720522, "strand": "-", "frame": 1, "gene": "marR", "product": "MarR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B85C5", "UniRef:UniRef100_O85035", "UniRef:UniRef50_O85035", "UniRef:UniRef90_O85035" ], "nt": "ATGAGAAAAGACGTTAGATTAGCTAACAAGGTAAAAGCAGCTAAAAGAGAACATAAATTAGCTTTAGAAAAAGCCAGAGCTCGCCGTAGACTTGCTAAACAACAAGCTGAATAA", "aa": "MRKDVRLANKVKAAKREHKLALEKARARRRLAKQQAE", "aa_hexdigest": "96ab6046e0d54d5a6a1bff54ed7fd2fb", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000B85C5", "uniref100_id": "UniRef100_O85035", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B85C5", "UniRef:UniRef100_O85035" ] }, "ips": { "uniref100_id": "UniRef100_O85035", "uniref90_id": "UniRef90_O85035" }, "psc": { "uniref90_id": "UniRef90_O85035", "product": "MarR family transcriptional regulator", "uniref50_id": "UniRef50_O85035" }, "pscc": { "uniref50_id": "UniRef50_O85035", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O85035" ], "product": "MarR family transcriptional regulator" }, "genes": [ "marR" ], "id": "DFOKDJOJIG_1264", "locus": "GCA013367855_01263" }, { "type": "cds", "sequence": "contig_1", "start": 720559, "stop": 720816, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACCGCTGAACATGCTTTGATTAAAAAATCCTATAATCAAGATATTCAACATTTAAATAATGATGAACAAGAAGAACTATTTCAAATATCTACAGAAGATATTGAGTTACCAAATGTTTCAAATAATGTTAATGACAACCAAAAAGATAAAAAAAATATAGCAACAAAAGAAAGCGTTAGTCAGAAAATACAAGAAATTGAAAAAATAGATCTTGAAACAATAATGCAAAAAATTAAACAAATTAAGAAAAAATAG", "aa": "MTAEHALIKKSYNQDIQHLNNDEQEELFQISTEDIELPNVSNNVNDNQKDKKNIATKESVSQKIQEIEKIDLETIMQKIKQIKKK", "aa_hexdigest": "e304d5266ccb11b39fdd09bf15f660c6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9951.088999999996, "isoelectric_point": 5.354881095886231 }, "id": "DFOKDJOJIG_1265", "locus": "GCA013367855_01264" }, { "type": "cds", "sequence": "contig_1", "start": 720994, "stop": 721494, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAATTAGGATATGCCAAAAAAGTTAATTTAAGAGATTTTGAAAATAAATCTTCTCTTAAAACAAAAACTTGCATGTCGTTTAAGGATGATAATGATGAATTAATAGATGCCCAAATTTCTAATGATGAAAAAATGCTATTTATTTTACTAAATAATGGTATGTATCATTTAGTTTCGGAAAACGAACTAAAGGTTGGAATTTCTTTGAAAGCAAGAGGCATTAGACTTCTTTTAAACTTATATAAACATCCTCAACTTCAAGTAAGTGGTTTTATAACAGTTTCAAAATACAACAATATAATTTATTTAACGCAAGGTGGCTATATAAAATGTTGGGATACTAGCAAATTAGAATCAACCACACGCAATACTCCAAAAATGTTGTTTACGCCACTAAAAAATAATATTTTAGGTCTTCAATCACTTGCTGTTACATTGAGCAATTTAAAAATGTTATACACTGATAATAATGGTAATTTGGCAGAATATGATTGA", "aa": "MSKLGYAKKVNLRDFENKSSLKTKTCMSFKDDNDELIDAQISNDEKMLFILLNNGMYHLVSENELKVGISLKARGIRLLLNLYKHPQLQVSGFITVSKYNNIIYLTQGGYIKCWDTSKLESTTRNTPKMLFTPLKNNILGLQSLAVTLSNLKMLYTDNNGNLAEYD", "aa_hexdigest": "91c324bb4104950f52556aa6aeac12c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18909.671100000003, "isoelectric_point": 9.1135892868042 }, "id": "DFOKDJOJIG_1266", "locus": "GCA013367855_01265" }, { "type": "cds", "sequence": "contig_1", "start": 721603, "stop": 721914, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGAAATAAAAAATCAATATTCAAAGCCAAGATTAACGGAAATTTCAGATCAAAAAATCGATAAAGAAATTGATCATAAATTATTGACAAAAAATGAAGATTTTTATTTATATATAACCAAAGATGGATATTATAAAAAAATAAGTTTAAAAGTTTATACTAGCAATGAATTAAGCACGTTCAAGTTAAAAGAAGAAGATAATGTTTTCTATTTTGATAAAGTAAACTCATTATCAAAGATATTATTCTTTACAAATTTAGGAAATTATTTTATTATTGATTGCCATTTATTTAAAGATTGCAATTGA", "aa": "MLEIKNQYSKPRLTEISDQKIDKEIDHKLLTKNEDFYLYITKDGYYKKISLKVYTSNELSTFKLKEEDNVFYFDKVNSLSKILFFTNLGNYFIIDCHLFKDCN", "aa_hexdigest": "4ad2c66b55dc1167e617b233a33f0d58", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12401.070599999985, "isoelectric_point": 6.718846321105957 }, "id": "DFOKDJOJIG_1267", "locus": "GCA013367855_01266" }, { "type": "cds", "sequence": "contig_1", "start": 721942, "stop": 723024, "strand": "-", "frame": 1, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B04E97" ], "nt": "ATGCATGGTAATAAAGGTTCAATTGATGATGACCCTGCCGCTGCAATGCGTTACACTGAATCAAGACTTGAAAAAATTAGTGAACTAATGTTGAGAGATTTAGACAGAAAAGTTGTAAAAATGGCTCCAAACTTTGATGACTCTGAATACGAACCAATTGTTTTGCCGGCCTTATTTCCTAATTTATTAGTTAACGGTGCTAAAGGAATTGCTGCTGGTTTTGCTACAGAAATCCCACCACATAATCTAGGCGAAGTTATTGATGCAACAATTGCATTAATCAAAAATCCTACAATATCAATTGAAGAATTAAGTGAAATAGTTAAAGGACCGGATTTCCCAACAGGAGCAATTATTAATGGTATAAATGAAATAAAAAAAGCTCTTTCAAGTGGGCAAGGTAGAATTACAATTTCTTCGAAATATCATTATGTTTATGATAAAAAAGATGAATCGAAAATTATTGGTATTGAAATAATTGAAATTCCTTTTGGGGTTGTTAAATCAAAATTAGTTGCCGACATTGATGCAATTGCAATAGATAAAAAAATTTCTGGTATTAAAGAAGTTTTGGACCAAACAGATAGAAACGGAATTTCAATATTTATTCAATTAGAAGACGGTGCAAATGCTGACGCAATAATTGCATACTTAATGAATAAAACCGAACTAAGTATCTCGTATAGTTATAACATGGTTGCAATCGACAATAACCGTCCAGTAATTCTAAATCTCTATAGTGCTTTAATTGCTTATTTAAGTCATTTAAAAGAAGTTAATATAAATGGTATTAATTATGATTTAAAGAAGTTTAAATTGAGGCTAGAAATAGTTGAAGGGTTCATTAAAGTAGCTGAAATTTCTGATGAAGTTATACATTTGATTAAAGAAAGCGATAACTCAAAAAAAGGTGTTATCCTAGCATTGATGAATAAATTTAAATTTAGTGAATTGCAAGCAACAGCGATTGCTGAATTAAGATTGTACAAGCTTTCAAGAATGGATCAAATTGAATTTCAAGAAGAAAAGAAAAACCTTGAAATTCAAATTGAAAATTGCAATAAATTATTAAATGATAAATGA", "aa": "MHGNKGSIDDDPAAAMRYTESRLEKISELMLRDLDRKVVKMAPNFDDSEYEPIVLPALFPNLLVNGAKGIAAGFATEIPPHNLGEVIDATIALIKNPTISIEELSEIVKGPDFPTGAIINGINEIKKALSSGQGRITISSKYHYVYDKKDESKIIGIEIIEIPFGVVKSKLVADIDAIAIDKKISGIKEVLDQTDRNGISIFIQLEDGANADAIIAYLMNKTELSISYSYNMVAIDNNRPVILNLYSALIAYLSHLKEVNINGINYDLKKFKLRLEIVEGFIKVAEISDEVIHLIKESDNSKKGVILALMNKFKFSELQATAIAELRLYKLSRMDQIEFQEEKKNLEIQIENCNKLLNDK", "aa_hexdigest": "205914d130ca573767ea57f0a559b380", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021B04E97", "query_cov": 0.8611111111111112, "subject_cov": 1.0064935064935066, "identity": 0.506, "score": 299.0, "evalue": 4.35e-97, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_UPI0021B04E97" }, "pscc": { "uniref50_id": "UniRef50_UPI0021B04E97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B04E97" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "DFOKDJOJIG_1268", "locus": "GCA013367855_01267" }, { "type": "cds", "sequence": "contig_1", "start": 723178, "stop": 723360, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "ATGAAAAAAGATAGAAAAGAAGAAATACAAGAAGTTACTGAAAACATTATTGAAAAAAATATGGCCGATATAATGTCTGATAGATTCGGGCGTTATTCAAAATACATTATTCAACAAAGAGCAATTCCTGATGCTCGTGATGGACTAAAACCTGTTCAACGTCGGATTTTATATTCAATGTGA", "aa": "MKKDRKEEIQEVTENIIEKNMADIMSDRFGRYSKYIIQQRAIPDARDGLKPVQRRILYSM", "aa_hexdigest": "8d3587316b5efea3fb752e3d36364935", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 21, "aa_cov": 0.35, "hmm_cov": 1.0, "evalue": 1.210035982537322e-10, "score": 36.42316436767578, "start": 40, "stop": 60 } ], "seq_stats": { "molecular_weight": 7217.295900000001, "isoelectric_point": 9.451403617858887 }, "id": "DFOKDJOJIG_1269", "locus": "GCA013367855_01268" }, { "type": "cds", "sequence": "contig_1", "start": 723423, "stop": 723533, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCCCGAAACTCGTTCAATAATTAAAGTTGATGTAGAAGATGAAATTTTAGTTGAAAGAAATGTTTCAACATTGATGAGTGACGACGTAATAGCAAGAAAACAATGA", "aa": "MNPETRSIIKVDVEDEILVERNVSTLMSDDVIARKQ", "aa_hexdigest": "099d210941b061bc58644c32aaae5669", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4144.682400000001, "isoelectric_point": 4.507070732116699 }, "id": "DFOKDJOJIG_1270", "locus": "GCA013367855_01269" }, { "type": "cds", "sequence": "contig_1", "start": 723543, "stop": 723737, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTTGTTGAAAATGGTATGGTTTATATTGCTTTGCCACCTCTTTATAAGATTTCAATAAAATCAAAAAATACACAGCCTCTTTATGCTTGGGATGAAGACCAATTAAAAGAAATTACTTCAAAATATACCAACTATGAAATTCAACGCTACAAAGGGCTTGGTGAGATGAATGCTGACCAGCTTTGA", "aa": "MKKLVENGMVYIALPPLYKISIKSKNTQPLYAWDEDQLKEITSKYTNYEIQRYKGLGEMNADQL", "aa_hexdigest": "62171427d03e6284d882c45f788b6bc4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7514.605900000001, "isoelectric_point": 7.907514762878418 }, "id": "DFOKDJOJIG_1271", "locus": "GCA013367855_01270" }, { "type": "cds", "sequence": "contig_1", "start": 723744, "stop": 724700, "strand": "-", "frame": 2, "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "COG:COG0187", "COG:L", "SO:0001217", "UniRef:UniRef50_Q59961", "UniRef:UniRef90_D1J8V0" ], "nt": "ATGCCAAATCTTACTATTGAATTTTATAATGAAAATACTGATGAACATGTAACATTTAAAACCAAAGATGGCATAAAAGAATATATAAATTTTGTTGGTGAAGGCAAAAAATTTATTAGTGAAATTTTTTATGCATCTGGTATGTGCAATTACGGCATTGAAATTGACTTGGCATTAGTATATACTGATGGATATAGTGAATCAATTTACAGTTTTGTAAATAATGTAAAGACTCGTGATGGTGGAACACATGAAACAGCACTTAAAACCGCATTAACTAAGACAATAAATGATTATGCTTCTAAAAATGGTTTAGCTAAGAAAATAACTTTTGAAGGATCGGATGTTAGAGAAGGAATAATTGCTGTTATTTCTTTAAAAGTCCCAGAAGAAATATTAGAATTTGTAGGTCAAACGAAAGATAAATTAGGTACGCCAGAGGCTAGAGAAGTTGTTGAAGATTTTGTAAGTCAAAAGTTTTATTTCTTTTTAAATGAAAACAAAATTGAAGCAGAAAAAATTATTTCTAAAATTAAAAAAGCATACGAAGCAAAAGTTGCAGCTAGAAACGCAAGAAATGAAGCACGTAAGATTAAAAATAAATTTGAGAATAGAAAGATTCTTTCAGGAAAATTAACTCCTGCTCAAAGTAAAAATCCTGCCATTAAAGAATTGTTTTTAGTTGAAGGCGATTCAGCTGGCGGTTCTGCTAAATTAGGAAGAAATAGAGTTACCCAGGCAATATTGCCACTTAGAGGTAAAGTTATTAACACTGATAAAGCAAAATTAAGCGATGTTTTAGCAAATGAAGAAATTGCAACAATAATTAATACAATCGGAGCAGGTATAGGCGAAGACTTCAATTTAAAAAATGCTCAATATCACAAAATTATTATTATGACAGATGCTGATACTGATGGTGCTCACATTCAAACATTACTATTAACATTCTTTTGA", "aa": "MPNLTIEFYNENTDEHVTFKTKDGIKEYINFVGEGKKFISEIFYASGMCNYGIEIDLALVYTDGYSESIYSFVNNVKTRDGGTHETALKTALTKTINDYASKNGLAKKITFEGSDVREGIIAVISLKVPEEILEFVGQTKDKLGTPEAREVVEDFVSQKFYFFLNENKIEAEKIISKIKKAYEAKVAARNARNEARKIKNKFENRKILSGKLTPAQSKNPAIKELFLVEGDSAGGSAKLGRNRVTQAILPLRGKVINTDKAKLSDVLANEEIATIINTIGAGIGEDFNLKNAQYHKIIIMTDADTDGAHIQTLLLTFF", "aa_hexdigest": "6e326102553164995e849329219f0ca6", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI001E534FD0", "query_cov": 0.9842767295597484, "subject_cov": 0.8368983957219251, "identity": 0.534, "score": 318.0, "evalue": 4.62e-104, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_A0A380FFC7" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 723441, "stop": 725000, "observations": { "mutation": [ 723545, 723746, 724781, 724886 ], "stop": [ 723545, 723746, 724781, 724886 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8V0", "query_cov": 1.0, "subject_cov": 0.49765258215962443, "identity": 1.0, "score": 610.0, "evalue": 3.59e-215, "gene_start": 1, "gene_end": 318, "reference_start": 196, "reference_end": 513, "reference_sequence": "MSLNNYEASDLKVLKGLDAVRKRPGMYIGSTDSSGLHHLIWEIFDNAIDEALSGFANKIIVTLKKDSSVIVEDNGRGIPVNKHESGKSGVELVFTELHAGGKFGDGAYKTSGGLHGVGSSVVNALSSKVEVTVWRDKKAYFTAFQQDTIIEKTHEISPTTKQGTKVQFWADFSIFKKSKYSPEIIKEKLREASFLMPNLTIEFYNENTDEHVTFKTKDGIKEYINFVGEGKKFISEIFYASGMCNYGIEIDLALVYTDGYSESIYSFVNNVKTRDGGTHETALKTALTKTINDYASKNGLAKKITFEGSDVREGIIAVISLKVPEEILEFVGQTKDKLGTPEAREVVEDFVSQKFYFFLNENKIEAEKIISKIKKAYEAKVAARNARNEARKIKNKFENRKILSGKLTPAQSKNPAIKELFLVEGDSAGGSAKLGRNRVTQAILPLRGKVINTDKAKLSDVLANEEIATIINTIGAGIGEDFNLKNAQYHKIIIMTDADTDGAHIQTLLLTFFWRYMKKLVENGMVYIALPPLYKISIKSKNTQPLYAWDEDQLKEITSKYTNYEIQRYKGLGEMNADQLWETTMNPETRSIIKVDVEDEILVERNVSTLMSDDVIARKQWINNNVDFSSEDDFKINNK" }, "paralog": false, "identity": 0.9922928709055877, "score": 1002.0, "evalue": 0.0, "description": "Internal stop codon at 723545, 723746, 724781, 724886. Nonsense mutation around 723545, 723746, 724781, 724886.", "psc": { "uniref90_id": "UniRef90_D1J8V0", "gene": "GyrB", "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_Q59961", "cog_id": "COG0187", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8V0", "UniRef:UniRef50_Q59961", "COG:COG0187", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_Q59961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q59961" ], "product": "DNA topoisomerase 4 subunit B" } }, "id": "DFOKDJOJIG_1272", "locus": "GCA013367855_01271" }, { "type": "cds", "sequence": "contig_1", "start": 724884, "stop": 725285, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGAGTTTAAATAATTACGAAGCTAGCGATTTAAAAGTTTTAAAAGGGTTAGATGCCGTCAGAAAAAGACCGGGAATGTATATTGGGTCTACTGATTCAAGTGGGCTTCATCACTTAATTTGGGAAATTTTTGATAATGCTATTGATGAAGCGTTGAGTGGGTTTGCAAATAAAATAATTGTAACTTTAAAGAAGGATTCTTCTGTTATTGTTGAAGACAACGGTAGGGGAATCCCTGTCAATAAACATGAGTCTGGAAAAAGTGGAGTTGAACTTGTCTTTACTGAGCTTCATGCTGGGGGTAAATTTGGCGATGGCGCTTATAAAACAAGTGGGGGATTACACGGAGTAGGATCCTCAGTTGTCAATGCCTTAAGTTCTAAAGTTGAAGTTACGGTTTGA", "aa": "MSLNNYEASDLKVLKGLDAVRKRPGMYIGSTDSSGLHHLIWEIFDNAIDEALSGFANKIIVTLKKDSSVIVEDNGRGIPVNKHESGKSGVELVFTELHAGGKFGDGAYKTSGGLHGVGSSVVNALSSKVEVTV", "aa_hexdigest": "5ba540597d72b901175e8134d81c6abe", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00037E1229", "query_cov": 1.0, "subject_cov": 0.8012048192771084, "identity": 0.677, "score": 187.0, "evalue": 1.9e-58, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A962E3V0" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 101, "aa_cov": 0.7368421052631579, "hmm_cov": 0.8514851485148515, "evalue": 1.6740495606893657e-17, "score": 59.5620231628418, "start": 32, "stop": 129 } ], "seq_stats": { "molecular_weight": 14056.700999999986, "isoelectric_point": 6.182343101501464 }, "id": "DFOKDJOJIG_1273", "locus": "GCA013367855_01272" }, { "type": "cds", "sequence": "contig_1", "start": 725339, "stop": 725797, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTGTTCTTTAAGTGATTTATTAGTAGTTAAATACTACTTCAACGAACCATATGATTCAATATTTAAATCTAATAATTTTATAAGAGATTTAAGATTAATAGGTAAGGCTAAGGATTATGTAATTGACTTTGCTTTTTGCGACAAAAATGTTATAAATTCAAATAAAAAAAATATATATAAATTAATTAAAAAAGAATTCAACAACGGCAAAGATTTAACATTGATTGTAGGCCACGATACCAACATTGCCACGACCTTGGCAATGTTAGACGTCAAAATGCCAGACCTTAAACAATTAGAAAAATATCCAATTGGGTCTAAATTAATTTTTGCAATTAATGATGACAATAGTTTTGATTTGTACATTGCTTTTTTTGACTTTAAAGACATTAGAAACTTTAATCTTTCAAATCCACAAATTATAAAAATCGCAAGCAAAATTTTTCTAAAATAA", "aa": "MACSLSDLLVVKYYFNEPYDSIFKSNNFIRDLRLIGKAKDYVIDFAFCDKNVINSNKKNIYKLIKKEFNNGKDLTLIVGHDTNIATTLAMLDVKMPDLKQLEKYPIGSKLIFAINDDNSFDLYIAFFDFKDIRNFNLSNPQIIKIASKIFLK", "aa_hexdigest": "11d164f2d1cea03320fb1dd3c7747c50", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17611.32629999999, "isoelectric_point": 8.939266777038576 }, "id": "DFOKDJOJIG_1274", "locus": "GCA013367855_01273" }, { "type": "cds", "sequence": "contig_1", "start": 725990, "stop": 726166, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCCAAAAGATTAGAGAATTTTTCAATATAACAAATGATATTGATGTTAAGGTGATGGCAAATTCAACGCAAAGAACTTTTCAAACTGCACAACTGCTTTCAATTGGTCTTTTTCCCGCTAAAAAGGTTGACATTCAATGTCAAGACTATTCATTCAAAAACGAGAATGAATGA", "aa": "MAQKIREFFNITNDIDVKVMANSTQRTFQTAQLLSIGLFPAKKVDIQCQDYSFKNENE", "aa_hexdigest": "a9cd68fc9b087a17866b79df139b359c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6729.584499999999, "isoelectric_point": 6.154548835754394 }, "id": "DFOKDJOJIG_1275", "locus": "GCA013367855_01274" }, { "type": "cds", "sequence": "contig_1", "start": 726441, "stop": 726698, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00044.30" ], "nt": "ATGAAAAAAACTAGAATTGCAATCAACGGTTTTGGTAGAATTGGTCGTCTAATTTTTAGAGAAATTTTTAATGACCCTGAATTTGAAATCGTTGCTATTAATGATTTAACAGATGCTGCTACATTAGCTCACCTATTGAAATACGATACAGCTCATGGAAAATTGAATGAAAATATTTCACACACAGAAAATTCTATTATTGTAGATGGCAAAGAATATCGTGTTTATAGTGAAAAAGATCCATTAAACCTACCTTGA", "aa": "MKKTRIAINGFGRIGRLIFREIFNDPEFEIVAINDLTDAATLAHLLKYDTAHGKLNENISHTENSIIVDGKEYRVYSEKDPLNLP", "aa_hexdigest": "8a2ede745bc0b33b3a92b3c754c39126", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BE2B32", "query_cov": 0.9529411764705882, "subject_cov": 0.826530612244898, "identity": 0.654, "score": 113.0, "evalue": 5.77e-31, "valid": false, "product": "glyceraldehyde 3-phosphate dehydrogenase NAD-binding domain-containing protein", "uniref50_id": "UniRef50_A0A2M8L0B5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00044.30", "name": "Gp_dh_N", "length": 81, "aa_cov": 0.9529411764705882, "hmm_cov": 1.0, "evalue": 2.9388468469320617e-32, "score": 106.64137268066406, "start": 5, "stop": 85 } ], "seq_stats": { "molecular_weight": 9685.897599999995, "isoelectric_point": 5.858588218688964 }, "id": "DFOKDJOJIG_1276", "locus": "GCA013367855_01275" }, { "type": "cds", "sequence": "contig_1", "start": 726933, "stop": 727376, "strand": "+", "frame": 3, "gene": null, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "nt": "TTGGAAAAAAACTTTGGTATTGAAAAAGGATTTATGACAACAGTTCACTCATATACAGCAGACCAAAGATTACAAGATGCTCCACACAAAGATTTAAGAAGAGCAAGAGCAGCTGCTTCAAACATGGTTCCAACAACAACAGGTGCTGCAATAGCTATTGGAAAAGTTATCCCTTCATTGGATAAGAAACTAAATGGACTATCTTTAAGAGTTCCAACAATAACTGGATCATTCGTTGATTTAACTGTTGAATTAAAGAAAGATGCCACTGTTCAAGAAATTAATGAAGCAATTAAAAAATCTGCTTCTGAATCATTAGAATATTCTACTGAACCAATTGTATCAAGTGATATTATTGGTGCAAAAGCAGGCTCAATATTTGACTCACTTTTAACAAGCGTGTTAGAAGTAGACGGCAAAAAACTATACAAAGTTTATGCATGA", "aa": "MEKNFGIEKGFMTTVHSYTADQRLQDAPHKDLRRARAAASNMVPTTTGAAIAIGKVIPSLDKKLNGLSLRVPTITGSFVDLTVELKKDATVQEINEAIKKSASESLEYSTEPIVSSDIIGAKAGSIFDSLLTSVLEVDGKKLYKVYA", "aa_hexdigest": "32d3a0bf25be8bdd953fd582a9364e55", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4XW52", "query_cov": 0.9251700680272109, "subject_cov": 0.8553459119496856, "identity": 0.654, "score": 171.0, "evalue": 4.52e-52, "valid": false, "product": "Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain protein", "uniref50_id": "UniRef50_A0A3B0PH09" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PH09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [], "id": "DFOKDJOJIG_1277", "locus": "GCA013367855_01276" }, { "type": "cds", "sequence": "contig_1", "start": 727471, "stop": 727959, "strand": "+", "frame": 1, "gene": null, "product": "Septicolysin", "db_xrefs": [ "RefSeq:WP_036433110.1", "SO:0001217", "UniParc:UPI000512AD2D", "UniRef:UniRef100_UPI000512AD2D", "UniRef:UniRef50_D1J8V3", "UniRef:UniRef90_D1J8V3" ], "nt": "ATGAAACTTTCAATAAAGGAAATAATTAAAAAAATTAAATTACTTGAATTGGATCTCGAAGATTTAAAAAAAGAAGAATCCAAAGAAAACTTTATTGTTTATTTAGAAAACAAAAGGCCAACTAACATTGAATATGATTATCAAGATTATTCAAACAAAATTAAAAATTTATATAATGAGATTTTTAAATTAAGAACATTGGTTCAAGTAACTAATATAAACACAATAACATCATACAAAAAATATTCTATATCAGAATTAATTATTTTATTAGCTCAAAAAAGTAATCAATTAGAAAGATTGCAAGAAATGCGCGATTGCAAGAAAATTTCTAGGGTTACTACAAATGACGGTCAAAATGAATATACAGAATATCTTTTTGACCCTAAAATGGTTGCAATTGAACTTAGAAACTTAAACAAAGAAATTTCTGAAATTCAAATTGCAATTGATAGTGCAAACATTAATACATTAGTTGAAGTTAAGTAA", "aa": "MKLSIKEIIKKIKLLELDLEDLKKEESKENFIVYLENKRPTNIEYDYQDYSNKIKNLYNEIFKLRTLVQVTNINTITSYKKYSISELIILLAQKSNQLERLQEMRDCKKISRVTTNDGQNEYTEYLFDPKMVAIELRNLNKEISEIQIAIDSANINTLVEVK", "aa_hexdigest": "339c032535441aeb6e5500ef9f2f24b9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000512AD2D", "ncbi_nrp_id": "WP_036433110.1", "uniref100_id": "UniRef100_UPI000512AD2D", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512AD2D", "RefSeq:WP_036433110.1", "UniRef:UniRef100_UPI000512AD2D" ] }, "ips": { "uniref100_id": "UniRef100_UPI000512AD2D", "uniref90_id": "UniRef90_D1J8V3" }, "psc": { "uniref90_id": "UniRef90_D1J8V3", "product": "Septicolysin", "uniref50_id": "UniRef50_D1J8V3" }, "pscc": { "uniref50_id": "UniRef50_D1J8V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8V3" ], "product": "Septicolysin" }, "genes": [], "id": "DFOKDJOJIG_1278", "locus": "GCA013367855_01277" }, { "type": "cds", "sequence": "contig_1", "start": 728207, "stop": 728389, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACAACAAAAGTATATATTGATTTTGAAGCAATTACCAACCCTTTTGCAAGATTAGTAAATTTGCCAAGTGGAACTCCTTATGCCTATACATTAGGTCTTTTAAATATTCACGGTCAATATCAAATTAAAACATTTATTATTGACTTTAAAGAACATCATAATTTAAATTCAATTTGA", "aa": "MNTTKVYIDFEAITNPFARLVNLPSGTPYAYTLGLLNIHGQYQIKTFIIDFKEHHNLNSI", "aa_hexdigest": "8990b4dae1576a3af97b68c45f302653", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6897.818599999997, "isoelectric_point": 6.8117780685424805 }, "id": "DFOKDJOJIG_1279", "locus": "GCA013367855_01278" }, { "type": "cds", "sequence": "contig_1", "start": 728368, "stop": 728619, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTTTTATTCTATTAAAATATGGAATGTTAAAAATTTTTCCTGTTAATTTTGACAATGACACAATTGTATTGGAAATTAAAGGTTCAACATTATTTTCAGGAAATAATTTTTTTAAGCATTTATTTTCTAAAACAGGATTGTGGCCTACAAATACAACTTCCTTTAAATTTACAGTATTATTGATCTCTAATATGTCAGAAACTATTGCATTTTTTATAGCAGTTCAAATTGAATTTAAATTATGA", "aa": "MIFFILLKYGMLKIFPVNFDNDTIVLEIKGSTLFSGNNFFKHLFSKTGLWPTNTTSFKFTVLLISNMSETIAFFIAVQIEFKL", "aa_hexdigest": "e0e6cf210eeddfcba62797912e742fe5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9581.257599999994, "isoelectric_point": 9.158652687072756 }, "id": "DFOKDJOJIG_1280", "locus": "GCA013367855_01279" }, { "type": "cds", "sequence": "contig_1", "start": 728762, "stop": 728962, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAATCTTTTGTTAAAAGAATTAAAACGTTATTCTCACGACGATGTCAACAAAATGCTTTATATTGTTTCTCATCCTGATGAAACAGAAACTCTACTAAAGGAACTTTTATACAAACGTGAATTAATAAAACAAATAAAAAATTTGGAACTTGATGACAATTTAACAATTAAAGAAATTAAAGAAAAAATTTGA", "aa": "MNKNLLLKELKRYSHDDVNKMLYIVSHPDETETLLKELLYKRELIKQIKNLELDDNLTIKEIKEKI", "aa_hexdigest": "c5f9e23ce5844c46f86b1d1e4514c0d0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7996.301299999995, "isoelectric_point": 6.728338432312013 }, "id": "DFOKDJOJIG_1281", "locus": "GCA013367855_01280" }, { "type": "cds", "sequence": "contig_1", "start": 729089, "stop": 729319, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATAATAATTGGCGAAATTATATTTAATTTAAATGGGCTACTTGACAATTTCATGGTTAGCCATATTGATGGCGGAATTGATGCCTTGGCTTATGCTAATACATATACGGGAATAATTTATACAATTTTCTTTTCTATTCAAGCAATTGGAGCAATGTTCGTGGGTCAATATTATGGCCGTAAAGACTTTGACAAAGTAAAGCAGATAATGAATTTAAGAATTTGA", "aa": "MPIIIGEIIFNLNGLLDNFMVSHIDGGIDALAYANTYTGIIYTIFFSIQAIGAMFVGQYYGRKDFDKVKQIMNLRI", "aa_hexdigest": "d3969568ee1c60af6e60a2100fd3071a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8568.956099999996, "isoelectric_point": 5.485781288146971 }, "id": "DFOKDJOJIG_1282", "locus": "GCA013367855_01281" }, { "type": "cds", "sequence": "contig_1", "start": 729494, "stop": 729820, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTAAATGAAACCGGACATTCAAAATATGCTTTTGTTTCGGCATGTTTAACAATGCTAACAAATGCTTCAATTAATTCTATTTGTTTATACGGATTTCATAAACCTGCATATTATGCTGCTTTTGGTTCAATTATTGCTGCGGCAGTTTGCTTTACAAGTGATTCTTTATTTACATATTACAAAGAAAGAAAAATTTTTATTAATGTATTTAAATTGTTTCATATTACAAAACCAATTGCAAAGCAATTATTAAAAAGAATTCCCGCCATGTTAATTGCCATTGCCGGAATGTTAACTTTACCTACAAGAATGATTATTTGA", "aa": "MLLNETGHSKYAFVSACLTMLTNASINSICLYGFHKPAYYAAFGSIIAAAVCFTSDSLFTYYKERKIFINVFKLFHITKPIAKQLLKRIPAMLIAIAGMLTLPTRMII", "aa_hexdigest": "dea4ca7d7358ced9b48335dafbb9f039", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12088.445999999982, "isoelectric_point": 9.676914024353028 }, "id": "DFOKDJOJIG_1283", "locus": "GCA013367855_01282" }, { "type": "cds", "sequence": "contig_1", "start": 729875, "stop": 730288, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTAATGCTGTTACAATACTTGGTTTAGTCGAAAGCCTTTCTTCCGTTGCTTCGGCAATAACTTCTGTTTGTGCAAGCAATGTTTCGTTTTTTGTTGCTACAAGATTAGGAGAGTCTGATTTTGAAGGCGCAGATAAGCATGCTCATTCATTGCGTGGATTTCATGCTCTGGCCGGATGTTGTATGTCTATATTAATGATTGCGGTTGTTTTTGGAATTGCATATTCTCCTGCTACAACAAAAGGTGTTGGTATCGGTGTCTCTCAAAGATTTTATGATGCACAAAGAAAAGATTACATTATTGAAATATTAAATAAATCTGATATTTTAAAAAATAGTGATTTATTAAACTCGCTTGGCAATGATTTTACATTCACAAAAGGCCAAAATTTGGCTTATGATCAATGA", "aa": "MGINAVTILGLVESLSSVASAITSVCASNVSFFVATRLGESDFEGADKHAHSLRGFHALAGCCMSILMIAVVFGIAYSPATTKGVGIGVSQRFYDAQRKDYIIEILNKSDILKNSDLLNSLGNDFTFTKGQNLAYDQ", "aa_hexdigest": "2411070d180b47396f7000b958de3422", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14602.500499999973, "isoelectric_point": 5.824484825134278 }, "id": "DFOKDJOJIG_1284", "locus": "GCA013367855_01283" }, { "type": "cds", "sequence": "contig_1", "start": 730478, "stop": 730600, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTATTATTACTTATGGAATTGTTATTCCATTGCATAAAACTTATACTGGATATAGTGATTTATCAATCCCTCTAGAACTTGCTTATTTTATGTTATTTGCATTTGACTTTATTAGATGA", "aa": "MIIITYGIVIPLHKTYTGYSDLSIPLELAYFMLFAFDFIR", "aa_hexdigest": "e111707ee70e59b0596c46a3641cc0cd", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4687.5587, "isoelectric_point": 5.3624975204467775 }, "id": "DFOKDJOJIG_1285", "locus": "GCA013367855_01284" }, { "type": "cds", "sequence": "contig_1", "start": 730750, "stop": 731055, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00561.26" ], "nt": "ATGTTATATGATTTAGTTTCACCATACAATTCAGGACATTTAAAGGTTAGTGATATTCATAGTATTTATTATGAAGAATCAGGAAATCCAAATGGCAAGCCAATTTTGTTTGTCCATGGTGGCCCAGGAGGGGGAACCAAACCTTCATGTCGTCAATTTTTTGACCCTGAATTTTATAGAATTATTTTATTTGATCAAAGAGGATGCGGACTTAGCATACCTAGTGCCGAAATCAAACAAAATACAACTCAAGATTTAATAGAAGATATTGAAAAATTACGGAAATTTTTAAACATTGACAAGTGA", "aa": "MLYDLVSPYNSGHLKVSDIHSIYYEESGNPNGKPILFVHGGPGGGTKPSCRQFFDPEFYRIILFDQRGCGLSIPSAEIKQNTTQDLIEDIEKLRKFLNIDK", "aa_hexdigest": "049e0eec9caeeee4c69317414b102aa2", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A1L4BUW4", "query_cov": 0.9702970297029703, "subject_cov": 0.9245283018867925, "identity": 0.633, "score": 138.0, "evalue": 1.23e-40, "valid": false, "product": "Proline iminopeptidase", "uniref50_id": "UniRef50_A0A1L4BUW4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00561.26", "name": "Abhydrolase_1", "length": 71, "aa_cov": 0.6831683168316832, "hmm_cov": 0.9859154929577465, "evalue": 2.21313797333978e-10, "score": 36.224388122558594, "start": 33, "stop": 101 } ], "seq_stats": { "molecular_weight": 11419.813099999985, "isoelectric_point": 5.849380302429199 }, "id": "DFOKDJOJIG_1286", "locus": "GCA013367855_01285" }, { "type": "cds", "sequence": "contig_1", "start": 731179, "stop": 731334, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATCAAAAAGGTGCTTCTTATTATTTTCCCGCTGAATATGAAGAATATTCAAACTTTATTCCTGAAAATGAAAGAGGCGATTTAATTAATGCTTATTATAAATATTTAAATTCAAGTGACCAAAATGTTGCAGAGCAAGCAGCATATCATTGA", "aa": "MYQKGASYYFPAEYEEYSNFIPENERGDLINAYYKYLNSSDQNVAEQAAYH", "aa_hexdigest": "e9321f42b69b295c7098dd81562c2c79", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6056.417700000004, "isoelectric_point": 4.375886344909667 }, "id": "DFOKDJOJIG_1287", "locus": "GCA013367855_01286" }, { "type": "cds", "sequence": "contig_1", "start": 731362, "stop": 731604, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTGCCTAATTTAGAAGAAATTTTATCCGATAAAAAAAGTAATTTAGAATTAGCAAGATTAGAAAATTATTATTTTGTTAATAAGATATTTTTAAATGACGATAATTTTATTTTAAATAACGCAAGCAAAATCAAAGAAATTCCAACGATATTAATTCATGGTCAATTTGATATGGATTGTCGTCCAGTTGGCGCATATTTATTGCATAAAGCCTTACCAAAATCAAAACTTTGA", "aa": "MKKLPNLEEILSDKKSNLELARLENYYFVNKIFLNDDNFILNNASKIKEIPTILIHGQFDMDCRPVGAYLLHKALPKSKL", "aa_hexdigest": "8eff35bcba50ad8ab78d3385e415356d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9289.797099999992, "isoelectric_point": 8.734515190124512 }, "id": "DFOKDJOJIG_1288", "locus": "GCA013367855_01287" }, { "type": "cds", "sequence": "contig_1", "start": 731781, "stop": 732326, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09371.15" ], "nt": "ATGAATTCAATAAATAACGTTGCTAAAAAATTAAATTTAAGCGAAAATCAAGTAAAAAATACATTATCTTTGTTATCAGAAGGAGCAACAGTACCATTTATTTCAAGATATAGGAAAAATATAACTGGTGGTTTAGACGAAGATCAAATTACAAAAATAAATGATTTATATGTATATGATGTTGAATTAAATAAAAGAAAAGAAGCCATATTGAATATATTGGCCGAAAATAAATTATTAAATGATGAATTAAAACAAAAAATCGAAGCTGCTGATACAAAACAAGCAGTTGAAAATATTTATGAACCATTTAAAATAGGTAAAAAAACAAAAGCATCAGACGCTATTGCTCTTGGTTTAAGTCCGTTGGCTGATGAAATTTTAAATAATAATGATGACAACTATAATCTTTATTATGCTGCTAAGAAATATATTAGTGATAAATTAAAAACTGAAGATGAAGTCTTACAACAAACTAAATTTATCATTGCTCAAAATATTTCTCAAGACCCTTCTACAAGAGAATATGTAAAAAATCAATTATGA", "aa": "MNSINNVAKKLNLSENQVKNTLSLLSEGATVPFISRYRKNITGGLDEDQITKINDLYVYDVELNKRKEAILNILAENKLLNDELKQKIEAADTKQAVENIYEPFKIGKKTKASDAIALGLSPLADEILNNNDDNYNLYYAAKKYISDKLKTEDEVLQQTKFIIAQNISQDPSTREYVKNQL", "aa_hexdigest": "fda9b6997e32972ffd4ac1a74bff54fa", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09371.15", "name": "Tex_N", "length": 73, "aa_cov": 0.40331491712707185, "hmm_cov": 1.0, "evalue": 2.4018282680817045e-29, "score": 96.57521057128906, "start": 2, "stop": 74 } ], "seq_stats": { "molecular_weight": 20543.994799999997, "isoelectric_point": 5.389893913269043 }, "id": "DFOKDJOJIG_1289", "locus": "GCA013367855_01288" }, { "type": "cds", "sequence": "contig_1", "start": 732600, "stop": 732839, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16921.10" ], "nt": "ATGCCTTCAATAATTAGAGAAATTAAAACCGAATTATTTGAAAGAGCTGAGGCAGAAGCTATTAAAGTATTTGCTTCAAATGTTGAACAAATGTTATTGAGTCCAGCAACTAAAAATAAAAGAATTTTAGCAATAGACCCTGCTTATGTTAATGGATGTAAAATTGCAGCCATTGATCAAAATGGTAACTTTTTAAAAGAAGGAATAATTTATCCTCATAATCACAATCAAGAACAATGA", "aa": "MPSIIREIKTELFERAEAEAIKVFASNVEQMLLSPATKNKRILAIDPAYVNGCKIAAIDQNGNFLKEGIIYPHNHNQEQ", "aa_hexdigest": "fc249100f8a5e0c5a4ba0b0df100781a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16921.10", "name": "Tex_YqgF", "length": 38, "aa_cov": 0.4810126582278481, "hmm_cov": 1.0, "evalue": 2.120304806926664e-12, "score": 43.23159408569336, "start": 40, "stop": 77 } ], "seq_stats": { "molecular_weight": 8895.121799999999, "isoelectric_point": 6.0693471908569325 }, "id": "DFOKDJOJIG_1290", "locus": "GCA013367855_01289" }, { "type": "cds", "sequence": "contig_1", "start": 732951, "stop": 733928, "strand": "+", "frame": 3, "gene": null, "product": "S1 RNA-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A502M9J9" ], "nt": "TTGCAAAAAAGACTTGAAAAAAATCCTCATGAAAAAATCAAATTTGTAATTGTAAGTGAAGTTGGTGCTTCTGTTTATTCAGCTTCAAAAATTGCCCAAGAAGAATTTCCGCAATTACATGTTGAATATAGATCAGCAATTCATATTGGCAGAAAGTTCCAAGATCCCTTAAATGAATTAATTAAAATAGATCCTAAATCAATTGGAATAGGACAATATCAACATGATGTTAACCAAAAGGAATTATCAAAACAATTAACCGAAAAATTAAATAAAGTTGTTAACCTAGTTGGTGTTGATCTAAATACAGCAACAAAAGTGATTTTATCTTATATATCAGGATTAAGTAATACCATAGCAAGCAATATTGTAAACTATAGAGAAGAAAACGGTTCATTTAAATCACGTGAAGAACTTAAAAAAGTGAAAGGACTTGGGCCAAAAGCATACGAACAATCAGTTGGATTTTTAAGAATACATGATTCTAATAATTTTTATGACAAGACTAATATTCACCCCGAAAGTTATAAATTAGCAGATACTATTGTCAATAAGCTAAATATAGATTTGAATAATATTGACAAAGAAAAATTATTATCCGTTGATAAAGAACAACTTGCAAAAGAATTAAATTCTGATAAATATAGCATTCAATTAATTATAGAATCATTGATTGCACCCGAAAAAGATATTAGAGATGACAAACCAGGATTTTTAGTATCAGATAAAGTCTTAACTATTGATGATATAACTCAAGGTCAAATAATTAAGGGTCAAATTCAAAATGTTACCGATTTTGGTGCCTTTGCGTTTATTGGATTAAAACAAAACGTTTTGATACATATAACCAATATGAAGAAGAAAGAAAATCAATTTATTAAACACCCTTTAGATGTATTAAATGTTGGTGATAATGTAAACATACAAATAATTTCTATTGACAAAGAACACAATAAAATTCAAGGAAAAATTATTTGA", "aa": "MQKRLEKNPHEKIKFVIVSEVGASVYSASKIAQEEFPQLHVEYRSAIHIGRKFQDPLNELIKIDPKSIGIGQYQHDVNQKELSKQLTEKLNKVVNLVGVDLNTATKVILSYISGLSNTIASNIVNYREENGSFKSREELKKVKGLGPKAYEQSVGFLRIHDSNNFYDKTNIHPESYKLADTIVNKLNIDLNNIDKEKLLSVDKEQLAKELNSDKYSIQLIIESLIAPEKDIRDDKPGFLVSDKVLTIDDITQGQIIKGQIQNVTDFGAFAFIGLKQNVLIHITNMKKKENQFIKHPLDVLNVGDNVNIQIISIDKEHNKIQGKII", "aa_hexdigest": "5cb27132aa2d5add4614cd6d4fb50fc7", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003593DDCE", "query_cov": 0.8615384615384616, "subject_cov": 0.9929078014184397, "identity": 0.7390000000000001, "score": 412.0, "evalue": 1.23e-142, "valid": false, "product": "helix-hairpin-helix domain-containing protein", "uniref50_id": "UniRef50_A0A502M9J9" }, "pscc": { "uniref50_id": "UniRef50_A0A502M9J9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A502M9J9" ], "product": "S1 RNA-binding domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_1291", "locus": "GCA013367855_01290" }, { "type": "cds", "sequence": "contig_1", "start": 734155, "stop": 734982, "strand": "+", "frame": 1, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "nt": "ATGTTTTATAGATATATTTCTGAAAATATTGCAACATACGCCAATAATCGCCAACGTCAAGCAGGAATTGAAGACTTTGATTATGCAACTTTATCAGATGAAGAAGCATTGACAGGCAGAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTACCTTCTGAATTGTTTATTAATGTTGTTAGAAATGCTACAACAAATAATTGTTTAAATGAAACGTTGGACAATATTTTTAAAAATATTGAATCATCAGCAAAGGGACAACAAAGTGAAAATGATTTTTCAGGTCTTTTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTGCAAGAAATAGCAGCCATGAAATTAGGCAATTACCAAGATAATTCAATAGATGCCTTTGGCGATGCTTATGAATATTTGATGTCAATGTATGCATCAAATGCTGGAAAATCAGGGGGTGAATATTTTACTCCCCAAGAAGTTTCAGAATTACTTACTAAAATAGCAGTTTTCAATAAGAAAAAAGTCAATAGAGTATATGACCCTGCATGTGGATCGGGTTCTTTACTTTTACAAACAATCAAGGTTCTTGGTAAAGAAAATATCAAGGATGGATTTTATGGCCAAGAAGTCAATTTAACAACATACAACTTATGCAGAATAAATATGTTTTTGCATGATATAGGATTTGATAAATTCAACATTTACAACGGCGACACATTGCTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTCATTGTTTCAAATCCTCCTTATTCAATTAAATGA", "aa": "MFYRYISENIATYANNRQRQAGIEDFDYATLSDEEALTGRDDLINEKGFFILPSELFINVVRNATTNNCLNETLDNIFKNIESSAKGQQSENDFSGLFNDVDVNSQKLGRSVDERNKKLAAILQEIAAMKLGNYQDNSIDAFGDAYEYLMSMYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIK", "aa_hexdigest": "2e8bd9915f92cd87d98c874c55f3c3d7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028EAAB7E", "query_cov": 1.0, "subject_cov": 0.8136094674556213, "identity": 0.665, "score": 370.0, "evalue": 4.71e-126, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7U9ST90", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9ST90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "DFOKDJOJIG_1292", "locus": "GCA013367855_01291" }, { "type": "cds", "sequence": "contig_1", "start": 735139, "stop": 735603, "strand": "+", "frame": 1, "gene": null, "product": "type I restriction-modification system subunit M", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0009307", "KEGG:K03427", "SO:0001217", "UniRef:UniRef50_A0A7X6YKI2", "UniRef:UniRef90_UPI001939D1E0" ], "nt": "ATGTATCGCGGAGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAAGCAACTTGTTTTTTGGAACAAGTATTTCAACATGCATAATGGTATTGAAAAAATCAAAAATTGACAACAATATCTTATTTATTGACGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAACAAGTTAACTAGTCAAAATATTAACAATATTATTGATTACTACAGCCAAAGAAAAGATATTTCTTATATTTCAAAATTAGCTAGTGTTGAAGAAATTAAGTCCAATTCATATAATTTATCAGTCAATAGTTATGTTGAAAAACAAGACACTTCAGAAAAAATAGATATTAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAATATTGAGAAGCGAAATTGACAAAATAGTTGCCGAATTAGAGGAATAG", "aa": "MYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYSQRKDISYISKLASVEEIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIEILRSEIDKIVAELEE", "aa_hexdigest": "5fa2f199d4c72076ab731bb98d8de922", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6V7S9", "query_cov": 0.9935064935064936, "subject_cov": 0.9807692307692307, "identity": 0.66, "score": 201.0, "evalue": 7.37e-64, "valid": false, "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A645HPD1" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 734839, "stop": 735603, "observations": { "mutation": [ 734980, 735091 ], "stop": [ 734980, 735091 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939D1E0", "query_cov": 1.0, "subject_cov": 0.4652567975830816, "identity": 0.981, "score": 277.0, "evalue": 2.46e-91, "gene_start": 1, "gene_end": 154, "reference_start": 178, "reference_end": 331, "reference_sequence": "MYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIKWEGEDNPLLINDQRFSPAGILAPKSKADFAFILHSLSWLATDGVAAIVCFPGIMYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQWKDISYISKLASVEEIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE" }, "paralog": false, "identity": 0.9803149606299213, "score": 484.0, "evalue": 2.23e-175, "description": "Internal stop codon at 734980, 735091. Nonsense mutation around 734980, 735091.", "psc": { "uniref90_id": "UniRef90_UPI001939D1E0", "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7X6YKI2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939D1E0", "EC:2.1.1.72", "UniRef:UniRef50_A0A7X6YKI2", "KEGG:K03427", "GO:0003677", "GO:0009007", "GO:0009307" ] } }, "id": "DFOKDJOJIG_1293", "locus": "GCA013367855_01292" }, { "type": "cds", "sequence": "contig_1", "start": 735606, "stop": 735728, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAATAGAAAAGTTAATTAAGGAACTATGTCCTAATGGAGTTGAATATAAAAAAATGCAGGATATCTGCGACATATTTGGAGGTTTTACTCCCCCAAAAAATCTTTCCGAATATTGA", "aa": "MSKIEKLIKELCPNGVEYKKMQDICDIFGGFTPPKNLSEY", "aa_hexdigest": "17503b2070c18c1ae7961e59159b3fec", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4608.3783, "isoelectric_point": 6.0849210739135735 }, "id": "DFOKDJOJIG_1294", "locus": "GCA013367855_01293" }, { "type": "cds", "sequence": "contig_1", "start": 735807, "stop": 736298, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "GTGGATAATAATGTCTTAAGAATAAAAAAAATTTTTAAAGCAAATTCAATTATATTATCTACTACCGCAACAATTGGTGAACATGCTATTGTATTAGAACCATTTTTTTGCAATCAACAATTTTCAATTTTTACTGTTAAAGATAAATTTGTGGCTAAAATAAACCCAATGTTTATGTTTTATTATTTTTTTATCTTTTCAAAAATCTTAAAGCAAAGATATGAAAACGTAAATTTACCTTTAATTAAAAAAGAGGAACTTGGGAATTTGTTATTCCCCATTCCTAATATTTCAATTCAAAATCAAATTGTTAATATTTTAGACAAGTTCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCTATAGAGATAAACAATATAATTACTATAGAAATAAATTACTAGACTTTGATAATAATAAAAATCTAATAGCCAAAATTATGAATAAAACAACTTCAGATTCTTTAAATAATTTAGTTAATTATTGA", "aa": "MDNNVLRIKKIFKANSIILSTTATIGEHAIVLEPFFCNQQFSIFTVKDKFVAKINPMFMFYYFFIFSKILKQRYENVNLPLIKKEELGNLLFPIPNISIQNQIVNILDKFTELTTELTTELTYRDKQYNYYRNKLLDFDNNKNLIAKIMNKTTSDSLNNLVNY", "aa_hexdigest": "81539ecbd0fb48755d7c52e6f09279f3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 113, "aa_cov": 0.6932515337423313, "hmm_cov": 0.9646017699115044, "evalue": 1.7715736986274823e-17, "score": 59.723304748535156, "start": 9, "stop": 121 } ], "seq_stats": { "molecular_weight": 19259.197500000006, "isoelectric_point": 9.214546775817869 }, "id": "DFOKDJOJIG_1295", "locus": "GCA013367855_01294" }, { "type": "cds", "sequence": "contig_1", "start": 736311, "stop": 736865, "strand": "+", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0A454CAF7", "UniRef:UniRef90_UPI00193A7487" ], "nt": "TTGTTTGATATTATAATACCTCCTAAAAAAATACCTATTAATGAAACAATGAATAAAGGAAAATATATAGTTATCAATCAAGGCGATGATAAAAAAATGTATACAAATGATGAAAATGCTGTTTTGCCAGCAAATGAATATATTTTGTTTGGTGATCATACAAGAAAAATTAAATTTATAAATCATACCTTTGCACAAGGTGGTGATGGTTTAAAAATTTTATTAAATAAAGATAAAACAAAAAATAATGTATCATCAAAATTTTTATTTTATTCATTAGATAATATTAATATACCTTCTTTAGGATATAAAAGACATTGGTCCTCAATAACGAATATCAAAATCCCTATCCCTCACATTTCCATCCAAAATAAAATTGTTGAAATTCTTGACAAACTTGAAACATACACCAAAAACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTTTGA", "aa": "MFDIIIPPKKIPINETMNKGKYIVINQGDDKKMYTNDENAVLPANEYILFGDHTRKIKFINHTFAQGGDGLKILLNKDKTKNNVSSKFLFYSLDNINIPSLGYKRHWSSITNIKIPIPHISIQNKIVEILDKLETYTKNIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGVLSKNYLLLLNEL", "aa_hexdigest": "9909a959d935eba301f2a0f60d77213b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 736050, "stop": 736829, "observations": { "mutation": [ 736296 ], "stop": [ 736296 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193A7487", "query_cov": 0.9347826086956522, "subject_cov": 0.41445783132530123, "identity": 0.977, "score": 345.0, "evalue": 1.72e-116, "gene_start": 1, "gene_end": 172, "reference_start": 243, "reference_end": 414, "reference_sequence": "MNKIEKLIKELCPNGVEYKKIGEISTLFSGMKGVSKKWSEEGNCKFIDYMNCYKNFRIDVSLLKNATVKSLNQNILQKNDILFTASSETPDECCISSIIDDYIEDKIFMDDHLFAIRILKDFLKEINPIYLNFYFHCVDFRKKVNKIAKGMTRFYIPKSDFLNIQIPLPPLEIQNQIVNILDKFTELTTELTTELTYRDKQYNYYRNKLLDFDNNKNLIAKIMNKTTSNSLNNLVNYWKILDLFDIIIPPKKIPINETMNKGKYIVINQGDDKKMYTNDENAVLPANEYILFGDHTRKIKFINHIFAQGGDGLKILLNKDKTKNNVSSKFLFYSLDNINIPSLGYKRHWSSITNIKIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGIK" }, "paralog": false, "identity": 0.9343629343629344, "score": 474.0, "evalue": 8.71e-170, "description": "Internal stop codon at 736296. Nonsense mutation around 736296.", "psc": { "uniref90_id": "UniRef90_UPI00193A7487", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A454CAF7", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193A7487", "EC:3.1.21.3", "UniRef:UniRef50_A0A454CAF7", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454CAF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAF7" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "DFOKDJOJIG_1296", "locus": "GCA013367855_01295" }, { "type": "cds", "sequence": "contig_1", "start": 736908, "stop": 737057, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGTGTGAAATAAAGCGAGGAAAAGTTTACTCAAAGGAATTCATAAAATTAAATAAAGGTGAATACCCTGTTTATTCATCTCAAAGTTTAAACGATGGAATTTTAGGAAAAATTGACAAATATGATTTTGATGGTGAATATGTAACATGA", "aa": "MCEIKRGKVYSKEFIKLNKGEYPVYSSQSLNDGILGKIDKYDFDGEYVT", "aa_hexdigest": "b0f70c5bb8111e7c075e0d3502c6a325", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 53, "aa_cov": 1.0, "hmm_cov": 0.9811320754716981, "evalue": 2.4423843655236296e-09, "score": 33.23988342285156, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 5670.380600000001, "isoelectric_point": 6.121525382995604 }, "id": "DFOKDJOJIG_1297", "locus": "GCA013367855_01296" }, { "type": "cds", "sequence": "contig_1", "start": 737097, "stop": 737477, "strand": "+", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0A929V0W2", "UniRef:UniRef90_UPI00215CCF5B" ], "nt": "ATGGGTAGATTCTCAATAACTAATGTGTGTGGAATATTGAGTGTTCCAAATAAATCAAAATTAAATGTGAAATATTTAAGCACTTGCTTATCTATGCAGACAAAAAAATTTGTAAACAAGGCAAGTGGAAATCCTAAACTTATGAGTAATATTATGGAAAATATTGAAATCCCAATCCCTCATATTTCCATCCAAAATAAAATTGTTGAAATTCTTGACAAACTTGAAACATACACCAAAAACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTATGA", "aa": "MGRFSITNVCGILSVPNKSKLNVKYLSTCLSMQTKKFVNKASGNPKLMSNIMENIEIPIPHISIQNKIVEILDKLETYTKNIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGVLSKNYLLLLNEL", "aa_hexdigest": "d550266f968f77bd091fd9a96118a520", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 736956, "stop": 737501, "observations": { "mutation": [ 737055, 737475 ], "stop": [ 737055, 737475 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215CCF5B", "query_cov": 1.0, "subject_cov": 0.6961325966850829, "identity": 0.976, "score": 242.0, "evalue": 3.95e-80, "gene_start": 1, "gene_end": 126, "reference_start": 48, "reference_end": 173, "reference_sequence": "MNKGEYPVYSSQSLNDGILGKIDKYDFDGEYVTWTTDGAYAGTVFYRIGRFSITNVCGILSVLNKSKLNVKYLSTCLSMQTKKFVNKASGNPKLMSNIMENIEIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGVLSKNYLLLLNELWGKIVNIV" }, "paralog": false, "identity": 0.9558011049723757, "score": 344.0, "evalue": 8.48e-123, "description": "Internal stop codon at 737055, 737475. Nonsense mutation around 737055, 737475.", "psc": { "uniref90_id": "UniRef90_UPI00215CCF5B", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A929V0W2", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215CCF5B", "EC:3.1.21.3", "UniRef:UniRef50_A0A929V0W2", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_A0A929V0W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A929V0W2" ], "product": "Restriction endonuclease subunit S" } }, "id": "DFOKDJOJIG_1298", "locus": "GCA013367855_01297" }, { "type": "cds", "sequence": "contig_1", "start": 737571, "stop": 738038, "strand": "+", "frame": 3, "gene": null, "product": "Restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0A454CAF2", "UniRef:UniRef90_A0A454CAF2" ], "nt": "ATGAACAAAGGGGATTATTTAGCAATAACTTCAGGAAATAAATCATTTTATGTCTCTGATTTTTTAGTTGATGGAGAATATATATTTGTTAATGATGGTGGGCAAGCAGACGTTAAATATAATCAAGGAAAAACAATGTATTCCGATCATGTGTTTGCGTTTAAAACAAATGAATGCAACACAAAATATCTTTACTTTTATTTATTGAATATTTCTAATTTTATTAACGAAAAATTATTTGTTGGTAGTGGATTAAAAAATTTAAATAAAAAAGAATTCTTGAATCTTAATATTGCAATTCCTCCTATTGAAATTCAAAATAAAATAGTTGAAATCCTCGATAAATTGCAAGCCTATACCAAAGACATTCAAACTGGTCTTCCTCTTGAAATTGACCAACGTAGAAAGCAATACGAACATTACAGGGATAAATTATTGAACTTTAAAGAAAAGAATACTAAAATTTAA", "aa": "MNKGDYLAITSGNKSFYVSDFLVDGEYIFVNDGGQADVKYNQGKTMYSDHVFAFKTNECNTKYLYFYLLNISNFINEKLFVGSGLKNLNKKEFLNLNIAIPPIEIQNKIVEILDKLQAYTKDIQTGLPLEIDQRRKQYEHYRDKLLNFKEKNTKI", "aa_hexdigest": "5458678f30518f8e7c7029c13318d70b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAF2", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.981, "score": 305.0, "evalue": 7.57e-105, "valid": true, "product": "Restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A454CAF2", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_A0A454CAF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAF2" ], "product": "Restriction endonuclease subunit S" }, "genes": [], "id": "DFOKDJOJIG_1299", "locus": "GCA013367855_01298" }, { "type": "cds", "sequence": "contig_1", "start": 738070, "stop": 738309, "strand": "+", "frame": 1, "gene": null, "product": "Type I restriction-modification system, restriction subunit R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H0F8" ], "nt": "ATGGAAAAATATAACATTATTGTTGAAAACGAGAATGATACAGTTGTTTCTGAATATAAACCTTTAGCGAATAAAGCAAAAGATTATCAAACAGAAAGTGAACTTGAAAGTCAATTCATATCAATGCTTAAAAGTCAAGGATATGAGTATTTGAATATTCATAATGAAGAAGATTTAATTAATAATTTAAGACAACAAATTGAATTGCTAAATAAAATAAATTTTTCAGATAATGAATGA", "aa": "MEKYNIIVENENDTVVSEYKPLANKAKDYQTESELESQFISMLKSQGYEYLNIHNEEDLINNLRQQIELLNKINFSDNE", "aa_hexdigest": "f86dd96550b00a3a396005d7a98c4ec0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A0F5H0F8", "query_cov": 1.0379746835443038, "subject_cov": 1.0, "identity": 0.512, "score": 69.7, "evalue": 4.61e-14, "valid": false, "product": "Type I restriction-modification system, restriction subunit R", "uniref50_id": "UniRef50_A0A0F5H0F8" }, "pscc": { "uniref50_id": "UniRef50_A0A0F5H0F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H0F8" ], "product": "Type I restriction-modification system, restriction subunit R" }, "genes": [], "id": "DFOKDJOJIG_1300", "locus": "GCA013367855_01299" }, { "type": "cds", "sequence": "contig_1", "start": 738532, "stop": 738795, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAATGGCATTCCAATGGTTCATATTGAATTAAAAAGAAGAGGGGTTGCTCTAAAAGAGGCTTTTAATCAAATCATTAGATATCAAAGAGATTCGTTTTGGGCTAGTAATGGTTTATATGAATATGTGCAAGTGTTTGTTATTTCAAACGGGGCAAATACCAAATATTATTCAAATACAACAAGATTACAACATGTTAATAAAAAATCAGATATGATAGATTCAAAAACTTCTAAGAGTTTTGAATTTACACATTTTTGA", "aa": "MVNGIPMVHIELKRRGVALKEAFNQIIRYQRDSFWASNGLYEYVQVFVISNGANTKYYSNTTRLQHVNKKSDMIDSKTSKSFEFTHF", "aa_hexdigest": "c5fc9c08793e399412bd90854a98a26c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10196.490599999994, "isoelectric_point": 9.695351982116701 }, "id": "DFOKDJOJIG_1301", "locus": "GCA013367855_01300" }, { "type": "cds", "sequence": "contig_1", "start": 738910, "stop": 739026, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTAGTAATGAGACCTTATCAAATTGTTGCTGTTGAAAAAATAATCAATAAAATAAATATTTCACACAATTATAAAAAATATGGAAGTATTGCAGCGGGTGGCTTTATTTGA", "aa": "MLLVMRPYQIVAVEKIINKINISHNYKKYGSIAAGGFI", "aa_hexdigest": "1c95e9d0dc4ab6e276ca285323d80814", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4294.1347000000005, "isoelectric_point": 9.873542594909669 }, "id": "DFOKDJOJIG_1302", "locus": "GCA013367855_01301" }, { "type": "cds", "sequence": "contig_1", "start": 739051, "stop": 740466, "strand": "+", "frame": 1, "gene": null, "product": "Type I restriction enzyme endonuclease subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7GZJ3" ], "nt": "TTGACCTCTTTTAAAACTGCAAAATTAGCCTCACAATTGCCTTATATAAATAAAGTAATTTTTGTTGTTGATAGAAGAGATTTAGACTATCAAACAATGAAGGAATATGACAAATTAGAAAAAGGCGCTGCAAATTCAAATCGTTCTACATCAATATTGCAAAGACAACTAGAATCTAATGACCCTAATAAAAAAATAATAATTACAACTATTCAAAAATTATCAATTTTTATAAAAAATAATAGAAATCATCCCGTTTTTGATAAAGAAGTTGTTTTAATATTTGATGAATGTCACCGCTCTCAATTTGGTTCAATGCATACAGCTGTTATTAAAAATTTTAAAAAATATTATTTATTTGGTTTTACCGGAACACCAATTTTTGCAGCTAATATCAATAAATCAAGTCCGGAAGTGATTAAAACAACCGAACAAGCTTTCGGCCAAAAATTGCACACTTATACAATAATAAATGCAATAAATGATAGAAACGTTTTGCCATTTAAGATCGAATATATTAAAACAATTGATGTTGATAAATCGCTAAACGATAAAGAGGATCAAAAAGTAAGAGATATTGATCGGGAAAGTGTTTTTTTAGATCAAAGAAGAATTAAACTAGTTGTTGAATACATATTGAAACATTTCGATCAAAAAACTAGGAGAAATTCCAATATAATTTATAGCCATTCAATATCTGATAATTTAGATAAAACTATTAAAAGAAATGTGAGCGGTTTTAATTCTATTATGGCGGTTTCTTCAATAGAAATGGCTAAAAAGTATTATTTAGAATTTAAAAATCAGCTTGCTCAAGATCCTAAAAACAATCTAAAAATTGCTTTAATATATTCGTATGGGGTTAATACCGAGGAATCATATGGCGGTTTAATCGATGAAGAGGATAATGAAAATACATTGCAATTAAGTAAGCCAGATAGAGATTTTTTGGATGATGCTATTAAAGATTATAACAATATGTTCAACACAAACTTTAATAGTGAAGGTGAACAATTTCAAATGTATTATAAAGATGTTTCTTTAAAACTAAAAAATAAAGATTTAGACATGCTAATTGTAGTAAACATGTTTTTAACTGGTTTTGACGCACCAACATTAAATACATTATGGGTTGATAAGAAACTAAGAGAACATGGTTTAATTCAAGCTTTTTCAAGAACAAATAGAATTCTAAATGATAAAAAGGTATATGGAAATATTGTATGTTTTAGAAATTTAGAAAAAGAAACTGACCAAGCAATTTCAATATTTGGCGATGGTGAAGCCAGTGGAATTGTTAAAATCAAAAATTTCGTTGACTATTATTATGGCTATAATGATAGTCAGCAAGTGCATCACAATGGTTATTGCGATTATATTCAAAAATTAAATAATGAATTTCCTTTAGATAATTGA", "aa": "MTSFKTAKLASQLPYINKVIFVVDRRDLDYQTMKEYDKLEKGAANSNRSTSILQRQLESNDPNKKIIITTIQKLSIFIKNNRNHPVFDKEVVLIFDECHRSQFGSMHTAVIKNFKKYYLFGFTGTPIFAANINKSSPEVIKTTEQAFGQKLHTYTIINAINDRNVLPFKIEYIKTIDVDKSLNDKEDQKVRDIDRESVFLDQRRIKLVVEYILKHFDQKTRRNSNIIYSHSISDNLDKTIKRNVSGFNSIMAVSSIEMAKKYYLEFKNQLAQDPKNNLKIALIYSYGVNTEESYGGLIDEEDNENTLQLSKPDRDFLDDAIKDYNNMFNTNFNSEGEQFQMYYKDVSLKLKNKDLDMLIVVNMFLTGFDAPTLNTLWVDKKLREHGLIQAFSRTNRILNDKKVYGNIVCFRNLEKETDQAISIFGDGEASGIVKIKNFVDYYYGYNDSQQVHHNGYCDYIQKLNNEFPLDN", "aa_hexdigest": "74c8dc88bc4f40b5c77ab8f18af7a5c2", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0026DF2CF8", "query_cov": 1.048832271762208, "subject_cov": 0.903107861060329, "identity": 0.583, "score": 531.0, "evalue": 7.5e-183, "valid": false, "product": "UvrB domain 3-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A7X7GZJ3", "kegg_orthology_id": "K01153" }, "pscc": { "uniref50_id": "UniRef50_A0A7X7GZJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7GZJ3" ], "product": "Type I restriction enzyme endonuclease subunit" }, "genes": [], "id": "DFOKDJOJIG_1303", "locus": "GCA013367855_01302" }, { "type": "cds", "sequence": "contig_1", "start": 740506, "stop": 741153, "strand": "+", "frame": 1, "gene": null, "product": "type I restriction endonuclease subunit R, EcoR124 family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A412PBS6", "UniRef:UniRef90_UPI00280A69CD" ], "nt": "TTGTTTGGTAAGGTATTAAAATTAAAAAATATTCTTGCTTCTTTTGACGAATTCATTGGTAAAGAAATCTTAAACGTTAGAGATTTTCAAAATTATTCAAGCAAATATTTGGATATAAAAGATGAATTTAAAAAGATATATAAAGAAGAAATTGCTTCAATTAACGATGATGTAATTTTTGAAACCGAATTATTAAAGACTTACGAAGTTAATATTGATTATATATTGCAAAAGGTAGAAGAGTTTAGAGGAAAGACAATTACTGAAATTAACAATTTAAGAAATGAAATATCTTTAGCTTCTTCTTCATCGCCTGAATTAAGAAGTAAAAAACAATTAATTGAAAAATTCGTTTCAACAATTAATAATAATGAACAAGATATCCCAACTAAATTTCAAGAATTTATGGAACTAGAAAAAAAGAAAGAGCTTGACAAAATTATTAGTGAAGAAAATTTAGATTTTGATAAAACCCATAAATTTATGGATAATTCGTTTGAGTTAGGAGAAATAAAAACTGCTGGTAGTGAGATTGGAGATTTATTGCCTAAAATGCCATTATTTGGTTCAAAAGGAAAAGAAAGATCTATTAAAAAGAAGGCAGTTTTAGAAAAATTTGAAAATTATTTCCAAAAATTTTATATTTAA", "aa": "MFGKVLKLKNILASFDEFIGKEILNVRDFQNYSSKYLDIKDEFKKIYKEEIASINDDVIFETELLKTYEVNIDYILQKVEEFRGKTITEINNLRNEISLASSSSPELRSKKQLIEKFVSTINNNEQDIPTKFQEFMELEKKKELDKIISEENLDFDKTHKFMDNSFELGEIKTAGSEIGDLLPKMPLFGSKGKERSIKKKAVLEKFENYFQKFYI", "aa_hexdigest": "1d1bc9a69935aaec211d0c99191cfdf6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 740206, "stop": 741153, "observations": { "mutation": [ 740464 ], "stop": [ 740464 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280A69CD", "query_cov": 1.0, "subject_cov": 0.5456852791878173, "identity": 0.995, "score": 405.0, "evalue": 8.8e-140, "gene_start": 1, "gene_end": 215, "reference_start": 180, "reference_end": 394, "reference_sequence": "MQLNKPDRDFLDGAIKDYNNMFNTNFNSEGEQFQMYYKDVSLKLKNKDLDMLIVVNMFLTGFDAPTLNTLWVDKKLREHGLIQAFSRTNRILNDKKVYGNIVCFRNLEKETDQAISIFGDGEASGIVKIKNFVDYYYGYNDSQQVHHNGYCDYIQKLNNEFPLDNWNVISEQKNKEFIILFGKVLKLKNILASFDEFIGKEILNVRDFQNYSSKYLDIKDEFKKIYKEEIASINDDVIFETELLKTYEVNIDYILQKVEEFRGKTITEINNLRNEISLASSSSPELRSKKQLIEKFVSTINNNEQDIPTKFQEFMELEKKKELDKIISEENLDFDKTHKFMDNSFELGEIKTAGSEIGDLLPKMPLFGSKGKERSIKKKAVLEKFENYFQKFYI" }, "paralog": false, "identity": 0.9968253968253968, "score": 622.0, "evalue": 7.85e-228, "description": "Internal stop codon at 740464. Nonsense mutation around 740464.", "psc": { "uniref90_id": "UniRef90_UPI00280A69CD", "product": "type I restriction endonuclease subunit R, EcoR124 family", "uniref50_id": "UniRef50_A0A412PBS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280A69CD", "UniRef:UniRef50_A0A412PBS6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A412PBS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A412PBS6" ], "product": "Type I restriction endonuclease subunit R" } }, "id": "DFOKDJOJIG_1304", "locus": "GCA013367855_01303" }, { "type": "cds", "sequence": "contig_1", "start": 741217, "stop": 741843, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "ATGCCTCCCTTTTTACTTAAAATAGATAAAAAAGGAATGAAAATGAGTGAAATAAAATTAACAGCAAAAGAAATTTACAAACAAATTTATAACCTTTCACAAATTAAAAAAATAGATCAAGCAGAAGTTGTTGAAGCTTTTAAAAATACAGTTACAAAGTTAATAACTGAAACTTATGACGAAGAAGCAGATCTTGAATTTATTTTTGATCAAGAAAATGACAATTTTGCAATTATTAATCATAACAAAGTTGTCGTTGGCGATCCAATAACAAGCGAAGAAAAAGATCGCTTAACAAGATGCATAGAAATACCATTAAGTGATGCTAAAAAAATAGATCCAAATGCAGAAATTGGAGATTCTCTTTCTCAAGAAATTAACTTTGAATATTTTAGTAAAAAAGACTATAACAAAATTCTTGCTAATTTCAGTCAAGATATTAAAAATTTAGAAAGAAAAATGACTTGTGAAAAATATGCAAGTGAAGTTGGAAATTCAACTAAGGCAAAAATTGTTTCTGTTAACAAAGGAAAAGTAAATCTTGAATTAAGAGACGGAACTTTGGCATTTATGCCTTCTAATTTTGTAAATCAAAGAATAATTTCAAAATTAAATCCAGGCGATTGA", "aa": "MPPFLLKIDKKGMKMSEIKLTAKEIYKQIYNLSQIKKIDQAEVVEAFKNTVTKLITETYDEEADLEFIFDQENDNFAIINHNKVVVGDPITSEEKDRLTRCIEIPLSDAKKIDPNAEIGDSLSQEINFEYFSKKDYNKILANFSQDIKNLERKMTCEKYASEVGNSTKAKIVSVNKGKVNLELRDGTLAFMPSNFVNQRIISKLNPGD", "aa_hexdigest": "4f9281516ccb8135e0fea36fb62d02dd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 130, "aa_cov": 0.625, "hmm_cov": 0.9153846153846154, "evalue": 5.812855127015703e-21, "score": 70.60208892822266, "start": 24, "stop": 153 } ], "seq_stats": { "molecular_weight": 23765.912400000016, "isoelectric_point": 5.478733253479004 }, "id": "DFOKDJOJIG_1305", "locus": "GCA013367855_01304" }, { "type": "cds", "sequence": "contig_1", "start": 742051, "stop": 742857, "strand": "+", "frame": 1, "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "db_xrefs": [ "COG:COG0195", "COG:K", "SO:0001217", "UniRef:UniRef50_D1J8W2", "UniRef:UniRef90_D1J8W2" ], "nt": "ATGGAAGAAATCGGCGCAATAATGGGACGTGATTCTGAAAGAATTTCAACAATATCTAGAAAACTTAATGGAGAAAAAATAGATGTAGTTTTATATTCAGATGATATAAAAGAATACATTAAAAATGCATTAAGTCCAGCAAAGGTTATTGATTTAATTGAAGTTCCATCAAAAAATTCTTATCCTTCATTTAATGTTATTGTTCCAACAATTCAACATACGCTTGCTATTGGAAAGAAAGGCCAAAATGTTTCTTTAGCTTCTGAATTAGTAAAAGCAAAATTAGATATTTTAAGCCAAGAACAAGCAGACGAAAGAGGCATTGAATACAATTTTGAAAATGGTAATATTACAAAAGAAGAAATTGAATTACTTGAAAGTGGTAAAAAACTTCATCACTCATTTAAACACCCAAGAACTATTGGCAATAAAAATGCTACAAACAAATTTAATACGTTTGAAAATAGTTTTAATATTGATGAATTTGATGAAGACCTTGCCGAAATGAGAAGAAAAGCTCAACAAAATGATAATATCTTTGAAAGACAAATGTTTTCAAATAGTCTAGATGAAGATTTAGAAAGAACTTTATCAGAACTAAATGACGACAAAGAAAATAATGTTGATGAAAATGACCTTGATCCTTATTCAAAATCTTTTGATGAATTAAATGAACAAAAAAATGATGAAGAAGAGGATTATGAAAAAATTACAGCAACAAAAATGAAAGATTTTAAACGTGATGAAGATTTAAGCAGTGGATTAGAAAACATTGATTTAAGTGATTTGGATGATGAAAAATGGTAA", "aa": "MEEIGAIMGRDSERISTISRKLNGEKIDVVLYSDDIKEYIKNALSPAKVIDLIEVPSKNSYPSFNVIVPTIQHTLAIGKKGQNVSLASELVKAKLDILSQEQADERGIEYNFENGNITKEEIELLESGKKLHHSFKHPRTIGNKNATNKFNTFENSFNIDEFDEDLAEMRRKAQQNDNIFERQMFSNSLDEDLERTLSELNDDKENNVDENDLDPYSKSFDELNEQKNDEEEDYEKITATKMKDFKRDEDLSSGLENIDLSDLDDEKW", "aa_hexdigest": "a35c24dfad7c37351a139c1efe5c67ae", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusA" ], "truncated": "5-prime", "pseudogene": { "start": 741751, "stop": 742857, "observations": { "mutation": [ 741841 ], "stop": [ 741841 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W2", "query_cov": 1.0, "subject_cov": 0.4908424908424908, "identity": 0.993, "score": 501.0, "evalue": 9.46e-175, "gene_start": 1, "gene_end": 268, "reference_start": 279, "reference_end": 546, "reference_sequence": "MPPFLLKIDKKGMKMSEIKLTAKEIYKQIYNLSQIKKIDQAEVVEAFKNTVTKLITETYDEEADLEFIFDQENDNFAIINHNKVVVGDPITSEEKDRLTRCIEIPLSDAKKIDPNAEIGDSLSQEINFEYFSKKDYNKILANFSQDIKNLERKMTCEKYASEVGNSTKAKIVSVNKGKVNLELRDGTLAFMPSNFVNQRIISKLNPGDWIDVVIEEVKEENSNAQIIVSSVESRLLQKLFEIEIPEISQGLISIVNIARIPGERAKVSIKKSNDAPETMEEIGAIMGRDSERISTISKKLNGEKIDVVLYSDDIKEYIKNALSPAKVIDLIEVPSKNSYPSFNVIVPTIQHTLAIGKKGQNVSLASELVKAKLDILSQEQADERGIEYNFENGNITKEEIELLESGKKLHHSFKHPRTIGNKNASNKFNTFENSFNIDEFDEDLAEMRRKAQQNDNIFERQMFSNSLDEDLERTLSELNDDKENNVDENDLDPYSKSFDELNEQKNDEEEDYEKITATKMKDFKRDEDLSSGLENIDLSDLDDEKW" }, "paralog": false, "identity": 0.9918478260869565, "score": 711.0, "evalue": 8.07e-260, "description": "Internal stop codon at 741841. Nonsense mutation around 741841.", "psc": { "uniref90_id": "UniRef90_D1J8W2", "gene": "NusA", "product": "Transcription termination/antitermination protein NusA", "uniref50_id": "UniRef50_D1J8W2", "cog_id": "COG0195", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W2", "UniRef:UniRef50_D1J8W2", "COG:COG0195", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8W2" ], "product": "Transcription termination/antitermination protein NusA" } }, "id": "DFOKDJOJIG_1306", "locus": "GCA013367855_01305" }, { "type": "cds", "sequence": "contig_1", "start": 742851, "stop": 743108, "strand": "+", "frame": 3, "gene": "ylxR", "product": "YlxR domain-containing protein", "db_xrefs": [ "COG:AJ", "COG:COG2740", "KEGG:K07742", "SO:0001217", "UniRef:UniRef50_D1J8W3", "UniRef:UniRef90_D1J8W3" ], "nt": "ATGGTAAAGTCTATACAAAGAAAGTCAATTGTTGATAATAAAATATATGATATAGATTTATTGCTTAGATTTTCTAAAGATAAAAACAATTTAATTAGCTTTGATCCTTACAAAAAGAACCCCGGAAGAGGCGCATATTGTTTAAACGATAGTGAACAAATTGAAATTCTATTTAAAAGAAGATTATTAAATAAAGCATTTAAGCAGAATATAGATATTCAAAAATACAACGAAATAAGGAAAGAGGTGGACGAATGA", "aa": "MVKSIQRKSIVDNKIYDIDLLLRFSKDKNNLISFDPYKKNPGRGAYCLNDSEQIEILFKRRLLNKAFKQNIDIQKYNEIRKEVDE", "aa_hexdigest": "50e290183594512d6394b48159039ae9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8W3", "query_cov": 1.0, "subject_cov": 0.85, "identity": 0.988, "score": 168.0, "evalue": 9.64e-53, "valid": true, "gene": "YlxR", "product": "YlxR domain-containing protein", "uniref50_id": "UniRef50_D1J8W3", "cog_id": "COG2740", "cog_category": "AJ", "kegg_orthology_id": "K07742" }, "pscc": { "uniref50_id": "UniRef50_D1J8W3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8W3" ], "product": "YlxR domain-containing protein" }, "genes": [ "ylxR" ], "id": "DFOKDJOJIG_1307", "locus": "GCA013367855_01306" }, { "type": "cds", "sequence": "contig_1", "start": 743105, "stop": 743848, "strand": "+", "frame": 2, "gene": null, "product": "Translation initiation factor IF-2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357QY70" ], "nt": "ATGAGTAAACAAAATATCCAAAAACGAAAATCAAACGTTAGCTCGTTAAAAGAGCAATTAACTGCTGGTGAAACAAAATTACAAGATGGCGTTTTTATATTTAATGGGCCATTGACAATTTCGGAATTTTCTCTTAAAATAAATAAAAATCCTACTGAAATATTGACCTATTATTTTAAAAAAGGAATAATCAAAACAATTAACACAACATTAAGCGAAGATGAAATAGCAGAACTTTGTTTTGAATTTAATTTAGATTTTAAAAAAGAAGAAAATGTTGATGCTCAAAATTTGTTTAATAAGCTTGAAATTGTTGAAGACCCAAATGATTTAGCTTCTAGACCCCCAATAGTAACAATTATGGGACATGTTGACCATGGTAAGACTACATTAATTGATAGGATAAGACATAGTAATGTTACTGAAACAGAATTCGGTGGTATTACTCAACACACCGGAGCATATCAAGTTGAATATCAAGGACGCAAAATTACATTTTTAGATACACCTGGTCATGAGGCCTTTACTGAAATGCGTTCAAGGGGAGCAAAAATTACTGATATAGTAGTCATTGTAGTTGCGGCAGATGATGGAGTTAAACCTCAAACTATTGAAGCAATAGACCATGCAAAAGCAGCCAATGTGCCAATTATTGTTTTTGTTAATAAAATGGATAAGCAGGGAGCCGATATTGAAAGAATAAAAAGCGAACTTGCTGATAACAATGTTTTATGTGAAGAATGA", "aa": "MSKQNIQKRKSNVSSLKEQLTAGETKLQDGVFIFNGPLTISEFSLKINKNPTEILTYYFKKGIIKTINTTLSEDEIAELCFEFNLDFKKEENVDAQNLFNKLEIVEDPNDLASRPPIVTIMGHVDHGKTTLIDRIRHSNVTETEFGGITQHTGAYQVEYQGRKITFLDTPGHEAFTEMRSRGAKITDIVVIVVAADDGVKPQTIEAIDHAKAANVPIIVFVNKMDKQGADIERIKSELADNNVLCEE", "aa_hexdigest": "602c93818d65bf5a6bd2e13b8c4b98da", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0025CBB306", "query_cov": 1.0242914979757085, "subject_cov": 0.8322368421052632, "identity": 0.534, "score": 263.0, "evalue": 1.58e-84, "valid": false, "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_A0A357QY70", "kegg_orthology_id": "K02519" }, "pscc": { "uniref50_id": "UniRef50_A0A357QY70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357QY70" ], "product": "Translation initiation factor IF-2" }, "genes": [], "id": "DFOKDJOJIG_1308", "locus": "GCA013367855_01307" }, { "type": "cds", "sequence": "contig_1", "start": 744047, "stop": 744922, "strand": "+", "frame": 2, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413", "KEGG:K02519", "SO:0001217", "UniRef:UniRef50_Q98R05", "UniRef:UniRef90_D1J8W4" ], "nt": "TTGCTACCTCGTGATTTCATTGTAGCTGGAGGGCAATATGGCAAGATTAGAAGTTTAACTTCTATTGATGGTAAGCCTATACAAAAAGCATTGCCTGGTACTCCATGTATAATTACTGGTTTAAGCAAAAACCCTCAATCGGGTGATAAATTTGTAGCTATTAGTGATGAAAAATTTGCTAAAAAATTAGCATCTGAAAAATCATATTTAGATAAATGTAAAGAATTAAATGAAAAAAATGCATTTTCTATTCAAGATGGAATAAAAACTGTAAACATCATTGTTAAGACAGATGTTCAAGGAACAGCAGAAGCAATTAAACAAACATTGCAAAAAATCAGAAATGATGAAATCGCAGTTAATGTTATAAGAGCAACTTCTGGTGAAGTAACAAAAGCAGATATCTTGCTTGCTGAAACTTCTAATGCAAGAATTTATTTATTCAACAATACAATAAATAACGAAATTAAAAAGGCAGCCGAATTAAAGAATATAAAAATCTTTAAACATAATATCATTTATAAAATGGTTGAAGAATTAGAAACTTTAATAACAACCTTAAAAGAACCTGTTTATGAAGAAAAACTAATTGGATCTGCAACCGTCCTACAACTATTCTTTTATTCAAAAATAGGCCAAATTGCAGGATGCATGATGCAAGAAGGCATTGTTAAGGAATTTAGTAAGGTCGAAGTATATAGACGCAATAAATTAATTTTTAAAGGACAAATTATTTCTCTAAAAAGAGGAAAAAATGATGTCAAATCTGTTGAAAAGGGTTTTGATTTTGGAACTCATGTCAAGGATTTTGATAAAATAGAAGTAGATGATGAATTAAAATTTTATGAGGATGTATTAGTCAATGAACACAATTAA", "aa": "MLPRDFIVAGGQYGKIRSLTSIDGKPIQKALPGTPCIITGLSKNPQSGDKFVAISDEKFAKKLASEKSYLDKCKELNEKNAFSIQDGIKTVNIIVKTDVQGTAEAIKQTLQKIRNDEIAVNVIRATSGEVTKADILLAETSNARIYLFNNTINNEIKKAAELKNIKIFKHNIIYKMVEELETLITTLKEPVYEEKLIGSATVLQLFFYSKIGQIAGCMMQEGIVKEFSKVEVYRRNKLIFKGQIISLKRGKNDVKSVEKGFDFGTHVKDFDKIEVDDELKFYEDVLVNEHN", "aa_hexdigest": "73a8c8ba2b840e93e45105048ad6add7", "start_type": "TTG", "rbs_motif": null, "genes": [ "infB" ], "truncated": "5-prime", "pseudogene": { "start": 743747, "stop": 744922, "observations": { "mutation": [ 743846 ], "stop": [ 743846 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W4", "query_cov": 1.0, "subject_cov": 0.4809917355371901, "identity": 0.993, "score": 557.0, "evalue": 1.69e-195, "gene_start": 1, "gene_end": 291, "reference_start": 315, "reference_end": 605, "reference_sequence": "MSKQNIQKRKSNVSSLKEQLTAGETKLQDGVFIFNGPLTISEFSLKISKNPTEILTYYFKKGIIKTINTTLSEDEIAELCFEFNLDFKKEENVDAQNLFNKLEIVEDPNDLASRPPIVTIMGHVDHGKTTLIDRIRHSNVTETEFGGITQHTGAYQVEYQGRKITFLDTPGHEAFTEMRSRGAKITDIVVIVVAADDGVKPQTIEAIDHAKAANVPIIVFVNKMDKQGADIERIKSELADNNVLCEEWGGNYQFVYGSALKNSNINELLEAIFLQADLLDLKANINRDPIGTIIESRIDKGRGVVSTVIVESGTLLPRDFIVAGGQYGKIRSLTSIDGKPIQKALPGTPCIITGLSKNPQSGDKFVAISDEKFAKKLASEKSYLDKCKELNEKNAFSIQDGIKTVNVIVKTDVQGTAEAIKQTLQKIRNDEIAVNVIRATSGEVTKADILLAETSNARIYLFNNTINNEIKKAAELKNIKIFKHNIIYKMVEELETLITTLKEPVYEEKLIGSATVLQLFFYSKIGQIAGCMMQEGIVKEFSKVEVYRRNKLIFKGQIISLKRGKNDVKSVEKGFDFGTHVKDFDKIEVDDELKFYEDVLVNEHN" }, "paralog": false, "identity": 0.9948849104859335, "score": 755.0, "evalue": 1.1e-275, "description": "Internal stop codon at 743846. Nonsense mutation around 743846.", "psc": { "uniref90_id": "UniRef90_D1J8W4", "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_Q98R05", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W4", "UniRef:UniRef50_Q98R05", "COG:COG0532", "COG:J", "KEGG:K02519", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R05" ], "product": "Translation initiation factor IF-2" } }, "id": "DFOKDJOJIG_1309", "locus": "GCA013367855_01308" }, { "type": "cds", "sequence": "contig_1", "start": 744909, "stop": 745142, "strand": "+", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0006364", "SO:0001217", "UniRef:UniRef50_A0A448ZXY5", "UniRef:UniRef90_D1J8W5" ], "nt": "ATGAACACAATTAATCACGAAAGAAAAACACAATTACTATTAGAATTAATTGGAAATAGTTTTTATGAATTAAAAGATTTTGATGTAACAAACATTTCAATAAATGATGTTATATTATCAAATGATGGTTCTCATGCCAAGATCTATGTAACTTTATTTAAAGATCAAAAAAGATATTTAGAAAAATTAACCACAATGACACCATTTATTCGTTCGGTTGTGGCAAAGAATTGA", "aa": "MNTINHERKTQLLLELIGNSFYELKDFDVTNISINDVILSNDGSHAKIYVTLFKDQKRYLEKLTTMTPFIRSVVAKN", "aa_hexdigest": "f25d477706714015a6172cd1e1eb10bd", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "rbfA" ], "truncated": "3-prime", "pseudogene": { "start": 744909, "stop": 745250, "observations": { "mutation": [ 745140 ], "stop": [ 745140 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W5", "query_cov": 1.0, "subject_cov": 0.6814159292035398, "identity": 1.0, "score": 154.0, "evalue": 2.25e-47, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MNTINHERKTQLLLELIGNSFYELKDFDVTNISINDVILSNDGSHAKIYVTLFKDQKRYLEKLTTMTPFIRSVVAKNWKYKVLPELIFEIDTVEEHAAKIDKILNKIEEEKNN" }, "paralog": false, "identity": 0.9911504424778761, "score": 220.0, "evalue": 8.4e-76, "description": "Internal stop codon at 745140. Nonsense mutation around 745140.", "psc": { "uniref90_id": "UniRef90_D1J8W5", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_A0A448ZXY5", "cog_id": "COG0858", "cog_category": "J", "go_ids": [ "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W5", "UniRef:UniRef50_A0A448ZXY5", "COG:COG0858", "COG:J", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXY5" ], "product": "Ribosome-binding factor A" } }, "id": "DFOKDJOJIG_1310", "locus": "GCA013367855_01309" }, { "type": "cds", "sequence": "contig_1", "start": 745271, "stop": 745543, "strand": "+", "frame": 2, "gene": null, "product": "Glucose-inhibited division protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1T4L5B9" ], "nt": "ATGACCAATAATTTTGACGCTATTGTAATTGGCGGTGGTCATGCTGGAATAGAAGCAACTTATGCTTTGGCAAAAAGAAATTTTAAAGTCGCATTGGTAACTTTAAACATTAATAGACTTGCTATGCTTCCTTGCAACCCCTCAATTGGTGGTTCTGCAAAAGGAATAATAACACGTGAAATTGATGCCCTTGGCGGAGTTCAAGGGTTTTTTGCTGATAATGCAATGATTCAAATTAAGATGCTAAATACATCTAAAGGCCCAGCAGTTTGA", "aa": "MTNNFDAIVIGGGHAGIEATYALAKRNFKVALVTLNINRLAMLPCNPSIGGSAKGIITREIDALGGVQGFFADNAMIQIKMLNTSKGPAV", "aa_hexdigest": "1a1f7e54074d6f0611af994f07d089c1", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0021AE77E7", "query_cov": 0.9555555555555556, "subject_cov": 0.8113207547169812, "identity": 0.733, "score": 130.0, "evalue": 8.79e-38, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A1T4L5B9" }, "pscc": { "uniref50_id": "UniRef50_A0A1T4L5B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1T4L5B9" ], "product": "Glucose-inhibited division protein A" }, "genes": [], "id": "DFOKDJOJIG_1311", "locus": "GCA013367855_01310" }, { "type": "cds", "sequence": "contig_1", "start": 745622, "stop": 746950, "strand": "+", "frame": 2, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "COG:COG0445", "COG:J", "GO:0002098", "GO:0008033", "KEGG:K03495", "SO:0001217", "UniRef:UniRef50_Q98QV8", "UniRef:UniRef90_D1J8W6" ], "nt": "TTGATTCAAGATGAAGCAAGCGATTTAATTGTTGAAGATGGAACTTGTGTCGGAATTGAAACACTTGAACATGGTAAACTATATTCAAAAGTTGTTATTATGACAACTGGTGTTTATATGAATTCAAGAATTTTGCGCGGTGAAAATATAAAATATGATGGACCCGATGGAGAAAAGACAAGTTCAACACTTTCAAAAAATTTAAAAAAATATGGCTTTGAAATAATAAGATTAAAAACTGGAACACCTTGCAGAATTTATACAGATTCAATAGATTTTTCAAAAGTTGAAAAAGAAGTTTTAGAAAAAAATGAACTTTGTTTTTCAAGCCGTAGTAATAAAAAACTAGATGAACAAACTTGCTGTTATTTAACACACACAACCTTAAAGACAAAAGAAATAATCTTAAAAAACTTAAAACGTAGTTCATTATATTCTGGAATCATAATTGGAATTGGTCCACGTTATTGTCCAAGTGTTGAAGATAAAATTGTTAGATTTCAAGAAAAAGATACACATCATATTTTCTTTGAACCAGAAACAGCACGTGGTGACATCATGTACATTAATGGGTTGTCTACATCAATGCCTGAAGATGTTCAAGATGAAATGATTGCATCTATTCCAGGTCTTGAACATGCAAGAGTTCAAAAATATGGTTATGCAATTGAATATGATGCAATTAATCCTTTGAATTTATACAAATCTCTTGAAAGCAAAATTTTAAAAAATTTCTTTTCAGCAGGTCAACCAAATGGAACGAGTGGTTATGAAGAAGCAGCAGCTCAAGGATTAATAGCAGGAATTAACGCCGCTAATAAACTTGATAATTTAGAACCACTAATCATAAAAAGAAATGACGGTTATATTGGTGTTTTAATTGATGACATTGTAACTAAAGGCACAAACGAACCTTATAGAATGCTAACAAGTAGGGCAGAATTTAGACTTCTTTTGAGAAATGATAACGTTGATGAACGTTTGTATAAATATGCATATGACAACAAAATGATAAGCAAAGAAGAACATGATAGGATTGAAGAAAAATATAAGATTATTGCCGAAACAGTAGAAAAATTAAAAAATGAATATGTTTCTTCTAAAAGTGATGTTGGTCAAAAGTACAATGTAACAAATGGTATGTCATATTTAAAATTACTTTCAAATCCCGAAGTAGATCCAGTTGATATTTTAGGTTCAAATTTTCCATATATTGATGAAATAACTATTCAAGTTCGTTTGTATGGATATTTAAAGAAACAAGAATCAGCAGCTGATAAAATGCTTAGATTGGAACAATTAAAATTACCAAAAGACATTGATTATTGA", "aa": "MIQDEASDLIVEDGTCVGIETLEHGKLYSKVVIMTTGVYMNSRILRGENIKYDGPDGEKTSSTLSKNLKKYGFEIIRLKTGTPCRIYTDSIDFSKVEKEVLEKNELCFSSRSNKKLDEQTCCYLTHTTLKTKEIILKNLKRSSLYSGIIIGIGPRYCPSVEDKIVRFQEKDTHHIFFEPETARGDIMYINGLSTSMPEDVQDEMIASIPGLEHARVQKYGYAIEYDAINPLNLYKSLESKILKNFFSAGQPNGTSGYEEAAAQGLIAGINAANKLDNLEPLIIKRNDGYIGVLIDDIVTKGTNEPYRMLTSRAEFRLLLRNDNVDERLYKYAYDNKMISKEEHDRIEEKYKIIAETVEKLKNEYVSSKSDVGQKYNVTNGMSYLKLLSNPEVDPVDILGSNFPYIDEITIQVRLYGYLKKQESAADKMLRLEQLKLPKDIDY", "aa_hexdigest": "820f609c203aacd9f7a1f43746ed372b", "start_type": "TTG", "rbs_motif": null, "genes": [ "mnmG" ], "truncated": "both", "pseudogene": { "start": 745322, "stop": 747091, "observations": { "mutation": [ 745541, 746948 ], "stop": [ 745541, 746948 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W6", "query_cov": 1.0, "subject_cov": 0.7293729372937293, "identity": 0.991, "score": 865.0, "evalue": 3.45e-314, "gene_start": 1, "gene_end": 442, "reference_start": 118, "reference_end": 559, "reference_sequence": "MTNNFDAIVIGGGHAGIEATYALAKRNFKVALVTLNINRLAMLPCNPSIGGSAKGIITREIDALGGVQGFFADNAMIQIKMLNTSKGPAVWSLRAQIDKEKYSEIILKDIEKQKNITLIQDEASDLIVENGTCIGIETLEHGKLYSKVVIMTTGVYMNSRILRGENIKYDGPDGEKTSSTLSKNLKKYGFEIIRLKTGTPCRIYTDSIDFSKVEKEVLEKNELCFSSRSNKKLDEQTCCYLTHTTLKTKEIILKNLKRSSLYSGIIIGIGPRYCPSVEDKIVRFQEKDTHHIFFEPETARGDIMYINGLSTSMPEDVQDEMIASIPGLEHARVQKYGYAIEYDAINPLNLYKSLESKILKNFFSAGQPNGTSGYEEAAAQGLIAGINAANKLDNLEPLIIKRNDGYIGVLIDDIVTKGTNEPYRMLTSRAEFRLLLRNDNVDERLYKYAYDNKMISKEEHDRIEEKYKIIAETVERLKNEYVSSKSDVGQKYNVTNGMSYLKLLSNPEVDPVDILGSNFPYIDEITIQVRLYGYLKKQESAADKMLRLEQLKLPKDIDYWSVDNLATEARQKLDKIRPETIGQAARISGINPADIQMLIFYLNNKR" }, "paralog": false, "identity": 0.9915110356536503, "score": 1149.0, "evalue": 0.0, "description": "Internal stop codon at 745541, 746948. Nonsense mutation around 745541, 746948.", "psc": { "uniref90_id": "UniRef90_D1J8W6", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_Q98QV8", "cog_id": "COG0445", "cog_category": "J", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W6", "UniRef:UniRef50_Q98QV8", "COG:COG0445", "COG:J", "KEGG:K03495", "GO:0002098", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV8" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" } }, "id": "DFOKDJOJIG_1312", "locus": "GCA013367855_01311" }, { "type": "cds", "sequence": "contig_1", "start": 747094, "stop": 747525, "strand": "+", "frame": 1, "gene": "rlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "db_xrefs": [ "COG:COG1576", "COG:J", "EC:2.1.1.177", "KEGG:K00783", "RefSeq:WP_020002370.1", "SO:0001217", "UniParc:UPI00036F4328", "UniRef:UniRef100_UPI00036F4328", "UniRef:UniRef50_Q98QV7", "UniRef:UniRef90_D1J8W7" ], "nt": "ATGAAATTAAATATTGTAGCAGTAGGTTCTCTAAGCAAAGAATATAAAACTATCTATGATCAATATCTTAAAAAAATTAGTTTTTTTTGTTCAATAAATTTGATTGAAATAAAAGAACAAGTTAACAAAAATATTGATTTAAAAATAGCCGAAGAAACAAAATTAATCCTAGAAAAAATTCCTAAAAACTCCAAAGTTTATTATTTATCATTAAGAGGCAAGAAACTTGATAGTGGCGAATTTTCAGATTTACTTATTGAAGATAATATTACTTTTGTAATAGGCGGTAGCAATGGAGTTGATGAAACATATTTTAAAAATAAAATATGTTTTTCAGATTTGACATTTCCTCATCAATTATTTAGAATAATGTTAATTGAACAAATTTATCGAGGATTTAGTATAAAAAACAACATAAAATATCACAAATAA", "aa": "MKLNIVAVGSLSKEYKTIYDQYLKKISFFCSINLIEIKEQVNKNIDLKIAEETKLILEKIPKNSKVYYLSLRGKKLDSGEFSDLLIEDNITFVIGGSNGVDETYFKNKICFSDLTFPHQLFRIMLIEQIYRGFSIKNNIKYHK", "aa_hexdigest": "715fa86346ee70e9b7f8188ecbabc58e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00036F4328", "ncbi_nrp_id": "WP_020002370.1", "uniref100_id": "UniRef100_UPI00036F4328", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036F4328", "RefSeq:WP_020002370.1", "UniRef:UniRef100_UPI00036F4328" ] }, "ips": { "uniref100_id": "UniRef100_UPI00036F4328", "uniref90_id": "UniRef90_D1J8W7" }, "psc": { "uniref90_id": "UniRef90_D1J8W7", "gene": "RlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "ec_ids": [ "2.1.1.177" ], "uniref50_id": "UniRef50_Q98QV7", "cog_id": "COG1576", "cog_category": "J", "kegg_orthology_id": "K00783" }, "pscc": { "uniref50_id": "UniRef50_Q98QV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV7" ], "product": "Ribosomal RNA large subunit methyltransferase H" }, "genes": [ "rlmH" ], "id": "DFOKDJOJIG_1313", "locus": "GCA013367855_01312" }, { "type": "cds", "sequence": "contig_1", "start": 747544, "stop": 748335, "strand": "+", "frame": 1, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4", "UniRef:UniRef90_A0A454C8Z1" ], "nt": "GTGGGGTTAATTCCAAATAAGGAAAAAAAGCAAGATCATAAAGGTCTTGCTTTTTTTCTATCATTCATAAAAAAAATAAGTTTAAAAAAACGAAGAGCACAACACAAGCACAGCTCTTCATACATTTACATAAATTATACCACGTGCTCTTCGTTTTTTTTAAAAAACTTAAAAATTTTTGAAAGGAATTTTATGGGTGCTTATAGTGAATTTATGGTAATGCAGCAAGAGGCTAAATTAAACAACGATTATAGCCTTGTGGATGCATATGAAAGAGATTTAAATTGGAGGAGAAATTATAGTAAAAAAATATATGACAAATTAAGCGACATAGCTATAAGCAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATGGTTATTAAATCCTTTAAAAATAAAAAAATCAAAATCATTGATCTAATAAATTTAGGGTTTAAAAAAACTTCTATAAAATCAGCTATAAAAAGACTTATTGAAATAAAAGTTTTAGATGGTGAGTTTTATAAAAAATATAAACTTCTAAAATTAAAAAAAATAGTACTAAAAAACCCTAAGGAAAATTACTGGATTTTAAATGGCTATGATGCCTTAAAAATCTTTCTTCTTAATGGTTTAAGTAATGCTATTTTATATGCAATAAATAGGCATAAATCAAAGCAACTAAATGCTAAAAAATTGCATTGTAAAATAAACTCAAGAAAAGTGATTTTACAAAATGCATATTATGCAAAATTTAACTGA", "aa": "MGLIPNKEKKQDHKGLAFFLSFIKKISLKKRRAQHKHSSSYIYINYTTCSSFFLKNLKIFERNFMGAYSEFMVMQQEAKLNNDYSLVDAYERDLNWRRNYSKKIYDKLSDIAISKILKSKIVISESYYQKLKTNKVLLEVFMVIKSFKNKKIKIIDLINLGFKKTSIKSAIKRLIEIKVLDGEFYKKYKLLKLKKIVLKNPKENYWILNGYDALKIFLLNGLSNAILYAINRHKSKQLNAKKLHCKINSRKVILQNAYYAKFN", "aa_hexdigest": "9888806883b49ae0a181b8923c514e9d", "start_type": "GTG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A454C8Z1", "query_cov": 1.0, "subject_cov": 0.8092307692307692, "identity": 1.0, "score": 499.0, "evalue": 3.98e-177, "valid": true, "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_A0A9Q9F2D4" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4" ] }, "genes": [], "id": "DFOKDJOJIG_1314", "locus": "GCA013367855_01313" }, { "type": "cds", "sequence": "contig_1", "start": 748357, "stop": 748521, "strand": "+", "frame": 1, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "RefSeq:WP_175972262.1", "SO:0001217", "UniParc:UPI00158EAE53", "UniRef:UniRef100_UPI00158C7902", "UniRef:UniRef50_A0A9Q9F2D4", "UniRef:UniRef90_A0A454C8Z1" ], "nt": "ATGAACAAAGCCATAGCCAATTATTTTGGAGTTGAATATTTGCAAATGTTTGGTATATTGCGTAGTCAAGAAAAAAAAGTTACAACATTAAAATTAGGAAATTTAAACATCAAATGTAATGTTTATAAATTTTCTACACAACGTTATTTGTTAATGCAAATTTAA", "aa": "MNKAIANYFGVEYLQMFGILRSQEKKVTTLKLGNLNIKCNVYKFSTQRYLLMQI", "aa_hexdigest": "cf685e3d1d55bbc71ebe10a3c2a6ac0f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158EAE53", "ncbi_nrp_id": "WP_175972262.1", "uniref100_id": "UniRef100_UPI00158C7902", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EAE53", "RefSeq:WP_175972262.1", "UniRef:UniRef100_UPI00158C7902" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C7902", "product": "MAGa4850 family ICE element protein", "uniref90_id": "UniRef90_A0A454C8Z1" }, "psc": { "uniref90_id": "UniRef90_A0A454C8Z1", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_A0A9Q9F2D4" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4" ] }, "genes": [], "id": "DFOKDJOJIG_1315", "locus": "GCA013367855_01314" }, { "type": "cds", "sequence": "contig_1", "start": 748931, "stop": 749038, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E466C14", "UniRef:UniRef90_UPI001E466C14" ], "nt": "GTGGTAGATTACATTACAAGACCAGATAAATGCGTTGTATTTAAAGAAGATGAAAATAATTATGATGCTATGAAACTACTTCTTACTAAGGAAGATAAAATAAATTGA", "aa": "MVDYITRPDKCVVFKEDENNYDAMKLLLTKEDKIN", "aa_hexdigest": "4319b445e246145b5e30e86e9d399845", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 748931, "stop": 749099, "observations": { "insertion": [ 749081 ], "mutation": [ 749036 ], "stop": [ 749036 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E466C14", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 1.0, "score": 73.9, "evalue": 7.88e-17, "gene_start": 1, "gene_end": 35, "reference_start": 1, "reference_end": 35, "reference_sequence": "MVDYITRPDKCVVFKEDENNYDAMKLLLTKEDKINWYRQHLKINQNSKQSVFITY" }, "paralog": false, "identity": 0.9636363636363636, "score": 105.0, "evalue": 8.21e-32, "description": "Internal stop codon at 749036. Frameshift due to insertion around 749081. Nonsense mutation around 749036.", "psc": { "uniref90_id": "UniRef90_UPI001E466C14", "uniref50_id": "UniRef50_UPI001E466C14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E466C14", "UniRef:UniRef50_UPI001E466C14" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E466C14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E466C14" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4192.786300000001, "isoelectric_point": 4.716124534606934 }, "id": "DFOKDJOJIG_1316", "locus": "GCA013367855_01315" }, { "type": "cds", "sequence": "contig_1", "start": 749567, "stop": 749971, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313EAF8", "UniRef:UniRef90_UPI001313EAF8" ], "nt": "TTGTGCCAAGAATTCATTAAATCTTCAAAAAGTATTTTAGATGAAGTTTTAAACAAAAAAAATATTTCATATTCTCGTATTTCTGATGAAAATAAGAAAAATATTGAAACAATAAAAAAATTCTTATTAAATTATGACAATGATTATTCAAAGCTCTTTAATTCTTATCAAAAACAAATTAATGATTTAAATAATTTAAACATCGAAGATAAATTTCTAAAACAAAGAATTTCAAAAATTATAAATAATGAAATGGATGATTTTCAAAAACAAGTTGGTAATAAGATAATCAAAACCTTACTAAATGCCTATAAGCAAAATAATAATGCATTTACTAGCCGAAAGAATTTCGCTTCATTTTTCAATGAATTTCTAAAAATATTTAGCCTAAATAGATCGGAATGA", "aa": "MCQEFIKSSKSILDEVLNKKNISYSRISDENKKNIETIKKFLLNYDNDYSKLFNSYQKQINDLNNLNIEDKFLKQRISKIINNEMDDFQKQVGNKIIKTLLNAYKQNNNAFTSRKNFASFFNEFLKIFSLNRSE", "aa_hexdigest": "4b09a3798400806a690ffc25e6783ff9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 749426, "stop": 750067, "observations": { "mutation": [ 749489, 749969 ], "stop": [ 749489, 749969 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001313EAF8", "query_cov": 1.0, "subject_cov": 0.6291079812206573, "identity": 0.985, "score": 247.0, "evalue": 1.67e-81, "gene_start": 1, "gene_end": 134, "reference_start": 48, "reference_end": 181, "reference_sequence": "MGLSIKYDEDYQKINALTKDIWNTRKQIVGIIANNFANSNEIISKSNLCQEFIKSSKSILDEVLNKKNISYSRISDKNKKNIETIKKFLLNYDNDYSKLFNSYQKQINDLNNLNIEDKFLKQRISKIINNEMDDFQKQVGNKIIKTLLNAYKQNNNAFTSRKNFASFFNEFLKIFSLNRSEWALRNLAEREFLAKDLKELELVEEFYRSKLVH" }, "paralog": false, "identity": 0.9812206572769953, "score": 406.0, "evalue": 8.09e-147, "description": "Internal stop codon at 749489, 749969. Nonsense mutation around 749489, 749969.", "psc": { "uniref90_id": "UniRef90_UPI001313EAF8", "uniref50_id": "UniRef50_UPI001313EAF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001313EAF8", "UniRef:UniRef50_UPI001313EAF8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001313EAF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313EAF8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15970.975899999985, "isoelectric_point": 9.44772891998291 }, "id": "DFOKDJOJIG_1317", "locus": "GCA013367855_01316" }, { "type": "cds", "sequence": "contig_1", "start": 750075, "stop": 750227, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAACAAGAAAAATTATCAAAATCAAAAATTATAGGAAAGACACTTCTTTCAATAATTGTTAGTACATTTTTGTTTTTTGTTCTAACTTTTATTATTAATGTTTTTATGGGCCAGAATAAAAATCCTGGACATTTTAAACTTTTTTGA", "aa": "MEEQEKLSKSKIIGKTLLSIIVSTFLFFVLTFIINVFMGQNKNPGHFKLF", "aa_hexdigest": "ba2bb57e9f1e8664bda2b7f300a10a08", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5777.879399999999, "isoelectric_point": 9.699671363830568 }, "id": "DFOKDJOJIG_1318", "locus": "GCA013367855_01317" }, { "type": "cds", "sequence": "contig_1", "start": 750585, "stop": 750752, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAATATTGAATTTATGGCACAACTTGATTATTCTAAAAAACCAAATTTAATAATTAGCGATCCAAAGAAAGAAATTATCAAATTTACAGGAGGCTTATTAACTGAAAACGATTACAAAATTTATGTAATTGATTTCTCTGACCCTAAATTATCTCTAAAATGA", "aa": "MPNIEFMAQLDYSKKPNLIISDPKKEIIKFTGGLLTENDYKIYVIDFSDPKLSLK", "aa_hexdigest": "9deac9918115d24a413a97288fdd0f20", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6364.380800000001, "isoelectric_point": 6.098903465270994 }, "id": "DFOKDJOJIG_1319", "locus": "GCA013367855_01318" }, { "type": "cds", "sequence": "contig_1", "start": 750710, "stop": 750895, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCATTAACTGCCTTACCTCAATTTAAACTTTCAATAACATTATTTATAACTTCTAACGATTTATTAATTCTTTCTTCGAAAGTCAAATTCTTGAATTTTTCACCATCAACCGCTAAATCTCATGCATATGAAAGAGGGTTTCATTTTAGAGATAATTTAGGGTCAGAGAAATCAATTACATAA", "aa": "MPLTALPQFKLSITLFITSNDLLILSSKVKFLNFSPSTAKSHAYERGFHFRDNLGSEKSIT", "aa_hexdigest": "b7d7e4f1fd7336945595f2e5dd80b01d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6859.8555, "isoelectric_point": 9.696512413024902 }, "id": "DFOKDJOJIG_1320", "locus": "GCA013367855_01319" }, { "type": "cds", "sequence": "contig_1", "start": 750897, "stop": 751037, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E578F32", "UniRef:UniRef90_UPI001E578F32" ], "nt": "ATGTGGATTAGCCAAGCTAAAAAAACATTAATTGTTATTACTAATTTCTTATTGCTATTAGGTATTGATGATAAAAATATTATTCATAAAAATAATTTTAATTTCGAAACAATAGCTTCATTATTAAATACTTCTCACTGA", "aa": "MWISQAKKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSH", "aa_hexdigest": "b1a13d795929f5c842693543b06bcbaf", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 750807, "stop": 751085, "observations": { "insertion": [ 750885 ], "deletion": [ 750916 ], "mutation": [ 750873, 751036, 751063 ], "stop": [ 750873, 751036, 751063 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E578F32", "query_cov": 0.8478260869565217, "subject_cov": 0.40625, "identity": 1.0, "score": 77.4, "evalue": 1.56e-17, "gene_start": 8, "gene_end": 46, "reference_start": 37, "reference_end": 75, "reference_sequence": "MTFEERINKSLEVINNVIESLNWGKALMAICGLAKPKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSHRENDKTISAWILYAKKSTWWQ" }, "paralog": false, "identity": 0.9347826086956522, "score": 147.0, "evalue": 7.22e-48, "description": "Internal stop codon at 750873, 751036, 751063. Frameshift due to insertion around 750885. Frameshift due to deletion around 750916. Nonsense mutation around 750873, 751036, 751063.", "psc": { "uniref90_id": "UniRef90_UPI001E578F32", "uniref50_id": "UniRef50_UPI001E578F32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E578F32", "UniRef:UniRef50_UPI001E578F32" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E578F32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E578F32" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5284.135999999999, "isoelectric_point": 8.231081581115724 }, "id": "DFOKDJOJIG_1321", "locus": "GCA013367855_01320" }, { "type": "cds", "sequence": "contig_1", "start": 751140, "stop": 752108, "strand": "+", "frame": 3, "gene": null, "product": "type IV secretory system conjugative DNA transfer family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF33FFC", "UniRef:UniRef90_UPI000AF33FFC" ], "nt": "TTGTCAAAAACTCCTAAAGAAACATTTGGTGGAATATTAGCCGAAGCACAAAGTGTTTTAAATGTATTCAATAATGATGAAAATTTAAAGATGGTTTTAAGAGACACAGAAAGTTTCAATTTAGTTGATATAGTGTCCGATCCTAAACCTTTTGCTATATTTATACATTATCCTGACCATAAACCTACATCTCACTTTTTAGTAAGTATGTTGGTAGAACAAATTTATCAATCGTTAATTGAACAAGCAAACAAAAATGATAACCTAAAATTAGACAGAAAGGCTTTCTTTCTATTGGATGAATTCGGAAATTTACCTGTTGTTGTAAATTTTGATAATAAACTTACAATTTGTCGTTCAAGAAATATTTTCTTCATGCTGGTAGTACAAGATTACAATCAAATTAAGAAATATAACATATATGGAGAAGGAACAGCCCAAGTAATTAAAAACTCTTGCCAAGTAGTATATTTTTTAGCCTCTAATGATGAGCAAACATTAAACGATATTTCAAAAGCGCTCGGAACAAAAGAGGTTAAATCAAAAAGTTATACAACAAGTGATAACCATTCAAGTTCTACTGAAAGTGAAAAATCCAAACCTGTCATGGAAGTAGCGGAACTTAAATCTAAAAAACCTGATGATTTAATAATTATAAAAAATGGTTATAAACCTTTATTAATTAAAACCTTATTGGCTTATAAATATTTCCCAAATGATAATTATGTTTATACCAGAAACGAAAGTAAGCTACTAACTAATGCGGATTTTGAATTATTTGATATTAGAAGTTTGGAAAAACTTATTAAATTTGATAATTCTAAACAAAAAAAACAAAATAATAAAAACCCAAAAGTTTTAGATGATAACAATTTAACTTATTCAGAAGATGTTGAAGATCCTGCGGATTTATCCCAAGAACAAAAAATTCAAAGAATGAAGATGGTTTTTGGAAAATTAGATCTTTGA", "aa": "MSKTPKETFGGILAEAQSVLNVFNNDENLKMVLRDTESFNLVDIVSDPKPFAIFIHYPDHKPTSHFLVSMLVEQIYQSLIEQANKNDNLKLDRKAFFLLDEFGNLPVVVNFDNKLTICRSRNIFFMLVVQDYNQIKKYNIYGEGTAQVIKNSCQVVYFLASNDEQTLNDISKALGTKEVKSKSYTTSDNHSSSTESEKSKPVMEVAELKSKKPDDLIIIKNGYKPLLIKTLLAYKYFPNDNYVYTRNESKLLTNADFELFDIRSLEKLIKFDNSKQKKQNNKNPKVLDDNNLTYSEDVEDPADLSQEQKIQRMKMVFGKLDL", "aa_hexdigest": "4bef7560e91a67b66c035952680274f4", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 750909, "stop": 752375, "observations": { "mutation": [ 751035, 751062, 751110, 752106, 752208, 752259 ], "stop": [ 751035, 751062, 751110, 752106, 752208, 752259 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF33FFC", "query_cov": 1.0, "subject_cov": 0.6598360655737705, "identity": 0.972, "score": 602.0, "evalue": 3.4e-214, "gene_start": 1, "gene_end": 322, "reference_start": 78, "reference_end": 399, "reference_sequence": "QAKKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSHWENDKTISAWILYAKKRALDDNNYYWKKIYNNIEDLSKTPKETFGGILAEAQSVLNVFNNDENLKMVLRDTESFNLVDIVSDPKPFAIFIHYPDHKPTSHFLVSMLVEQIYQSLIEQANKNDNLKLDRKAFFLLDEFGNLPVVVNFDNKLTICRSRNIFFMLVVQDYNQIKKYNIYGEGTAQVIKNSCQVVYFLASNDEQTLNDISKALGTKEVKSKSYTTSDNHSSSTESEKSKPVMEVAELKSKKPDDLIIIKNGYKPLLIKTLLAYKYFPNDNYVYTRNESKLLTNADFELFDIRSLEKLIKLEKSKPKNQDNKNLKVLDDNNLTYSEDVEDPDDLSQEQKIQRMKMVFGKLDLWNNYINLKNDYKRTLIPHKKNQIKEQIEETLSLMWEQYVTNDLESDLLNILWSEAKERKIPNYHDLNVDILLYLLHPTEDISKLIEKSN" }, "paralog": false, "identity": 0.9713114754098361, "score": 926.0, "evalue": 0.0, "description": "Internal stop codon at 751035, 751062, 751110, 752106, 752208, 752259. Nonsense mutation around 751035, 751062, 751110, 752106, 752208, 752259.", "psc": { "uniref90_id": "UniRef90_UPI000AF33FFC", "product": "type IV secretory system conjugative DNA transfer family protein", "uniref50_id": "UniRef50_UPI000AF33FFC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF33FFC", "UniRef:UniRef50_UPI000AF33FFC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF33FFC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF33FFC" ], "product": "type IV secretory system conjugative DNA transfer family protein" } }, "id": "DFOKDJOJIG_1322", "locus": "GCA013367855_01321" }, { "type": "cds", "sequence": "contig_1", "start": 752387, "stop": 752752, "strand": "+", "frame": 2, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6N2ZW67", "UniRef:UniRef90_UPI0034A1F568" ], "nt": "ATGAATATAAATAAAATAAAAGTTGTTGAATTATTTGGCGGAATAGGCGCTATTAGAAAAGCTCTAATTAGAACCATCAAACCTCCTAATTTTGAAATTATTGATTATGTCGAAAGTGATAAAAAAACAGTAAATGCTTATAATGCAATATATAACGATTTTTACAAACCTTTAGATATAAGAGATTATTCACTTCCTAATAATGTTAATGTTGATTTATTGTTTCACGGATCTCCTTGTCAAGATTTTTCGTGTGCAGGTAAAAATATCGGAGGAAGTAAAGAAAGTAAAAGTCGAAGTAGTTTATTGTGGGAAACAATTAGAATCATTAAAGAAGCTCAAATAAAACCCAAAATTGTTATCTGA", "aa": "MNINKIKVVELFGGIGAIRKALIRTIKPPNFEIIDYVESDKKTVNAYNAIYNDFYKPLDIRDYSLPNNVNVDLLFHGSPCQDFSCAGKNIGGSKESKSRSSLLWETIRIIKEAQIKPKIVI", "aa_hexdigest": "eaf6fad3de3dcb6e7243cee8bcd6a62c", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0034A1F568", "query_cov": 0.8925619834710744, "subject_cov": 0.9230769230769231, "identity": 0.981, "score": 218.0, "evalue": 7.63e-72, "valid": true, "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_A0A6N2ZW67" }, "pscc": { "uniref50_id": "UniRef50_A0A6N2ZW67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6N2ZW67" ], "product": "DNA (cytosine-5-)-methyltransferase" }, "genes": [], "id": "DFOKDJOJIG_1323", "locus": "GCA013367855_01322" }, { "type": "cds", "sequence": "contig_1", "start": 753191, "stop": 753388, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00145.23" ], "nt": "ATGATTATAGATAATAAACCATATTATTTAAGGGTTATTACACCATTAGAAGCAATGCTATTAATGGGCTATGATCAGCAAGATTATGAAAGAATTAAGAATTTCTGTGATAGTCAAATTTATAAGTTTGCTGGAAATTCTATTGTTGTTAATGTATTGCAAGAAATAATTAAACACATTTTTAGACACAAACAATAA", "aa": "MIIDNKPYYLRVITPLEAMLLMGYDQQDYERIKNFCDSQIYKFAGNSIVVNVLQEIIKHIFRHKQ", "aa_hexdigest": "60bdb35f264425eba9a2a021602d885f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00145.23", "name": "DNA_methylase", "length": 56, "aa_cov": 0.8615384615384616, "hmm_cov": 0.9464285714285714, "evalue": 9.34828096425361e-11, "score": 37.29964828491211, "start": 6, "stop": 61 } ], "seq_stats": { "molecular_weight": 7807.056499999999, "isoelectric_point": 7.875280570983887 }, "id": "DFOKDJOJIG_1324", "locus": "GCA013367855_01323" }, { "type": "cds", "sequence": "contig_1", "start": 753610, "stop": 753798, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6", "UniRef:UniRef90_UPI00158CD6B8" ], "nt": "ATGGAAGCAGATTTATGGGCGTTTGACATTAATAACATTTATGAAATGCAAAACGATTTTTATGATATTGAATTCGTGGGCATTGACGCCTATGGTAGAGCCTTCATCATAAGCAGAGAAGATGCAGAAAAATATTATGCAAAGTACTATTTAAAACAAAACAAGTTAGTTGAAAAAGGAGATAAATAA", "aa": "MEADLWAFDINNIYEMQNDFYDIEFVGIDAYGRAFIISREDAEKYYAKYYLKQNKLVEKGDK", "aa_hexdigest": "2b989bd0783900700549016f6cdfd84f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 753397, "stop": 753798, "observations": { "mutation": [ 753463, 753574 ], "stop": [ 753463, 753574 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158CD6B8", "query_cov": 1.0, "subject_cov": 0.46616541353383456, "identity": 0.9840000000000001, "score": 128.0, "evalue": 7.17e-37, "gene_start": 1, "gene_end": 62, "reference_start": 72, "reference_end": 133, "reference_sequence": "MTKDKYCAFCGKKIVGMYVKNFWSDFCSREFRAKSEKLQEDLNYWKFHEPQDVYPLNDAWELADKELRKYGMEADLWAFDINNIYEMQNDFYDIEFVGIDAYGRAFVISREDAEKYYAKYYLKQNKLVEKGDK" }, "paralog": false, "identity": 0.9548872180451128, "score": 269.0, "evalue": 4.87e-95, "description": "Internal stop codon at 753463, 753574. Nonsense mutation around 753463, 753574.", "psc": { "uniref90_id": "UniRef90_UPI00158CD6B8", "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158CD6B8", "UniRef:UniRef50_A0A6A8PZW6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7473.2735999999995, "isoelectric_point": 4.464725685119628 }, "id": "DFOKDJOJIG_1325", "locus": "GCA013367855_01324" }, { "type": "cds", "sequence": "contig_1", "start": 754260, "stop": 754559, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAATGAAGAATATGTTTATGTAAATCCCTATTTAGAAATAGAACCAATTAATATTAAAGAAATTAAAGACGATTACATAGAAGCATTAAAAACTTTTGATTTAGATGACCTTTTAGAATCAAGAGATGAAAAATCATTAATTAATTTAGATCCTAACAAATCTTCTAAATCATCTTTATCTGATTTTGACAAGACCCCAACCGATCTCAAAGATTCCAAAGTAATCAACGAATATAACAATTCATCCCATACCCCCTCTGATACCCAATCAAAATCCCATTCATTAACAAAATAA", "aa": "MSNEEYVYVNPYLEIEPINIKEIKDDYIEALKTFDLDDLLESRDEKSLINLDPNKSSKSSLSDFDKTPTDLKDSKVINEYNNSSHTPSDTQSKSHSLTK", "aa_hexdigest": "a6561f80382dc09bd9ef82e293825631", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11374.318299999984, "isoelectric_point": 4.538900566101075 }, "id": "DFOKDJOJIG_1326", "locus": "GCA013367855_01325" }, { "type": "cds", "sequence": "contig_1", "start": 754569, "stop": 754784, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAATTTTCAATCGTGATCAAATGGAACAAATCCACATTGGTTTAGTCCAAGGTCTTGATGTCACTCAATACACTCAACTAGACCCTCAAGGCCATCCAATATTCAATGATAATCAAATGACCGAAATCCGTAAGGGTCTAAAAAAAGGCCTTGATGTCTCTAAATATAATCAAATAGATCATGATGGATATCCGATATTTAATTGA", "aa": "MKKKIFNRDQMEQIHIGLVQGLDVTQYTQLDPQGHPIFNDNQMTEIRKGLKKGLDVSKYNQIDHDGYPIFN", "aa_hexdigest": "50c77cce3fde287027f172e412b761ee", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8302.352699999998, "isoelectric_point": 6.8166093826293945 }, "id": "DFOKDJOJIG_1327", "locus": "GCA013367855_01326" }, { "type": "cds", "sequence": "contig_1", "start": 754791, "stop": 754883, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGTAATCCGTGAAGGTCTAGAACAAGGCCTTGACATCTCACAATACACCCAACTAGACCTCAAAGGCCATCCAATCTATGATTTTTGA", "aa": "MYVIREGLEQGLDISQYTQLDLKGHPIYDF", "aa_hexdigest": "f392ce09b464ff1f92d3e9b1614ffcd7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3542.9643000000005, "isoelectric_point": 4.427268791198731 }, "id": "DFOKDJOJIG_1328", "locus": "GCA013367855_01327" }, { "type": "cds", "sequence": "contig_1", "start": 754887, "stop": 755369, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAATCCGCATTGGCCTAGAACATGGCCTTGACATCTCAATTTATGCAAAACAAAACAATTTTAATAGACCTATCTATGATTGTTTGCAAATGTCTGAAATCCGTGAAGGTCTAGAACAAGGCCTTGATATATCTCAATACAGCCAACTAGACCATAAAGGTCGCCCAATCTTTAATCGAGGTCAGATGTCTGAAATCCGTGAAGGTCTAGAACAAGGCCTTGATGTCTCACAATACACTCAACTAGACCTTCAAGGCCATCCAATATTCAATGGTTATCAAATGTATGCAATCCGTAAGGGTCTAGAACAAGGGCTTGACATCTCTCAATACACCCATTTGGCCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTCAAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCGGAATCCCAATCAAAATCCCATTCATTAACAAAATAA", "aa": "MEQIRIGLEHGLDISIYAKQNNFNRPIYDCLQMSEIREGLEQGLDISQYSQLDHKGRPIFNRGQMSEIREGLEQGLDVSQYTQLDLQGHPIFNGYQMYAIRKGLEQGLDISQYTHLASKSSLSDFDKTPTDLKDSKVINEYNKSSHTPSESQSKSHSLTK", "aa_hexdigest": "488f3e3c4bdf69e043f8da199ac57e64", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18269.103100000008, "isoelectric_point": 5.644987297058106 }, "id": "DFOKDJOJIG_1329", "locus": "GCA013367855_01328" }, { "type": "cds", "sequence": "contig_1", "start": 755575, "stop": 755790, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAACAATTTTTGATAATGACCAAATGGAACAAATCTGCCTTGGTCTAGAACACGGCCTTGATATCTCTAAATATACTCAATTAAATCATAAAGGCCACCCTATATTTGATAGCTTCCAAATGAATGAAATCCGCGAAGGTCTAGAACACGGCCTTGATGTCACCCAATACACTCAACTAGACCCTCAAGGCCACCCCATCTATGATTGA", "aa": "MKKTIFDNDQMEQICLGLEHGLDISKYTQLNHKGHPIFDSFQMNEIREGLEHGLDVTQYTQLDPQGHPIYD", "aa_hexdigest": "010d3ad0acfd0d45522343f67a270a79", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8273.155999999995, "isoelectric_point": 4.917618751525878 }, "id": "DFOKDJOJIG_1330", "locus": "GCA013367855_01329" }, { "type": "cds", "sequence": "contig_1", "start": 755893, "stop": 756384, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAATCCGCATTGGCATAGAACAAGACCTTGATATATCTCAATATAACAAACTAGACCCTCAAGGCCATCCAATTTTCGTTGGTAGCCAAATGTCTGAAATTCGTTATGGCCTAAAACAAGGCCTTGATGTCTCTAAATATACCCAACTAGACTCTCAAGGCCACCCTATATTTGACAGCTTTCAAATGGGTGAAATCCGTAAGGGTCTAAAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGATTTTTGCCAAATGGAACAAATCCGTGAAGGCATAAAACAAGGCCTTGACGTATCTAAACCCACTCAATTAGTCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCCCACTCACAACCTAATCAAAATAAAACCTCAGAATCAAAAAATATAAATAGATGCATATAG", "aa": "MEQIRIGIEQDLDISQYNKLDPQGHPIFVGSQMSEIRYGLKQGLDVSKYTQLDSQGHPIFDSFQMGEIRKGLKQGLDISKYTQLDPNGHPIYDFCQMEQIREGIKQGLDVSKPTQLVSKSSLSDFDKTPTDLQDSKVINEYNKSHSQPNQNKTSESKNINRCI", "aa_hexdigest": "838b3a0f7ae0a3d7c528f71ab940495b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18550.55810000001, "isoelectric_point": 5.783390235900878 }, "id": "DFOKDJOJIG_1331", "locus": "GCA013367855_01330" }, { "type": "cds", "sequence": "contig_1", "start": 756375, "stop": 756683, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175972201.1", "SO:0001217", "UniParc:UPI00158A229D", "UniRef:UniRef100_UPI00158A229D", "UniRef:UniRef50_A0A6A8PZR1", "UniRef:UniRef90_A0A6A8PZR1" ], "nt": "ATGCATATAGATGAATTTATTGATAAATATCGCAGGAATTTAAAACAATCGGGTTTTAATAAAGACAATATAGACTTCTTTTTATATGGTTTAGAACTAGGATTAAATATAAAAATAAAATCCAGTAGAAATTATTACAACTTTAAAAAATCTATACATTATTCTTCAAATAATTCTCATCCCTCTTCCCTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCGGAATCCCTATCAAAATCCCATTCATTAACAAAATAA", "aa": "MHIDEFIDKYRRNLKQSGFNKDNIDFFLYGLELGLNIKIKSSRNYYNFKKSIHYSSNNSHPSSLSDFDKTPTDLQDSKVINEYNKSSHTPSESLSKSHSLTK", "aa_hexdigest": "046a545343a2a85e9a188a55c5994240", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158A229D", "ncbi_nrp_id": "WP_175972201.1", "uniref100_id": "UniRef100_UPI00158A229D", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158A229D", "RefSeq:WP_175972201.1", "UniRef:UniRef100_UPI00158A229D" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158A229D", "uniref90_id": "UniRef90_A0A6A8PZR1" }, "psc": { "uniref90_id": "UniRef90_A0A6A8PZR1", "uniref50_id": "UniRef50_A0A6A8PZR1" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZR1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11882.013199999987, "isoelectric_point": 9.098890495300292 }, "id": "DFOKDJOJIG_1332", "locus": "GCA013367855_01331" }, { "type": "cds", "sequence": "contig_1", "start": 756693, "stop": 757004, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAATTTTCAATAGTGATCAAATGAATGAAATCCGCATTGGCCTAGAAGAAGGTCTAGACGTATCCAAATACATCCAATTATCCCCTCACGGTCGCCCAATCTATGATAGTTATCAAATGTATGCAATTCGTAAGGGTCTAAAACAAGACCTTGATATCTCTCAATATACCCAATTAAATCATAAAGGTCGCCCAATCTATGATGGTTATGAAATGTCTGTAATCCGTGAAGGTTTAAAAGCTGGCCTTGATGTCTCTCAATATAACCAACTAGACCCTCAAGGCCACCCAATTTTTGATTGA", "aa": "MKKKIFNSDQMNEIRIGLEEGLDVSKYIQLSPHGRPIYDSYQMYAIRKGLKQDLDISQYTQLNHKGRPIYDGYEMSVIREGLKAGLDVSQYNQLDPQGHPIFD", "aa_hexdigest": "126f5412d616ec80c3270f843a2a49d3", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11950.412999999993, "isoelectric_point": 6.2719213485717775 }, "id": "DFOKDJOJIG_1333", "locus": "GCA013367855_01332" }, { "type": "cds", "sequence": "contig_1", "start": 757011, "stop": 757346, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCAATCCGTGAAGGTCTAGAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGCTGGTTATGAAATGTCTGTAATCCGTGAAGGTCTAGCCCAAGGCCTTGATATATCTCAATATAACCAACTAGACCCTCAAGGCCACCCAATTTTCGTTGGTAGCCAAATGCACGAAATTCGTAAGGGTCTAGAACAAGGCCTTGATATATCTCAATATACCCAATTAAATCATAAAGGCCATCCAATCTATAATGCTTACCAAATGCACGAAATTCGCATTGGCCTACTATTTGATTTTTTAGGATCATGA", "aa": "MYAIREGLEQGLDISKYTQLDPNGHPIYAGYEMSVIREGLAQGLDISQYNQLDPQGHPIFVGSQMHEIRKGLEQGLDISQYTQLNHKGHPIYNAYQMHEIRIGLLFDFLGS", "aa_hexdigest": "41904fd1a9cbc9c37b395101b508c202", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12619.068699999993, "isoelectric_point": 5.332713890075683 }, "id": "DFOKDJOJIG_1334", "locus": "GCA013367855_01333" }, { "type": "cds", "sequence": "contig_1", "start": 757520, "stop": 757711, "strand": "+", "frame": 2, "gene": null, "product": "DUF1508 domain-containing protein", "db_xrefs": [ "RefSeq:WP_036439043.1", "SO:0001217", "UniParc:UPI000513025C", "UniRef:UniRef100_A0A454C8Y5", "UniRef:UniRef50_A0A9Q9F2Q3", "UniRef:UniRef90_A0A454C8Y5" ], "nt": "GTGTCAAAATACTTTTATAGATTAAGAGGGAGTCAATCTACAGGGAAGAATGCCAAAACATATTCGTTTGGCTCTGCGCAACAATACAATAAAGCATTAATAATGGCTCAAGATGCTATTTCAAAAAATCAATTTAAGGAGGTGCAAATAGAAAGAATACAAGTTATAAAAATTATTAAGCAAAATTCATAG", "aa": "MSKYFYRLRGSQSTGKNAKTYSFGSAQQYNKALIMAQDAISKNQFKEVQIERIQVIKIIKQNS", "aa_hexdigest": "bb61ae58264773743c7974dccf1b1a0d", "start_type": "GTG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000513025C", "ncbi_nrp_id": "WP_036439043.1", "uniref100_id": "UniRef100_A0A454C8Y5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513025C", "RefSeq:WP_036439043.1", "UniRef:UniRef100_A0A454C8Y5" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C8Y5", "uniref90_id": "UniRef90_A0A454C8Y5" }, "psc": { "uniref90_id": "UniRef90_A0A454C8Y5", "uniref50_id": "UniRef50_A0A9Q9F2Q3" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2Q3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2Q3" ], "product": "DUF1508 domain-containing protein" }, "genes": [], "id": "DFOKDJOJIG_1335", "locus": "GCA013367855_01334" }, { "type": "cds", "sequence": "contig_1", "start": 757725, "stop": 757835, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAACAATTATTCTTTTAATTATCATAATTAGACATTTGAAAAAACGCAATAAATTATTAGCAAATTCTAACCAAATTAATAGCGCTAAAGGAGTGATAAATAAATAA", "aa": "MITIILLIIIIRHLKKRNKLLANSNQINSAKGVINK", "aa_hexdigest": "eefd1c41d5c8ceca7425f556f70597b0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4096.028700000002, "isoelectric_point": 11.999967765808105 }, "id": "DFOKDJOJIG_1336", "locus": "GCA013367855_01335" }, { "type": "cds", "sequence": "contig_1", "start": 757990, "stop": 758307, "strand": "+", "frame": 1, "gene": null, "product": "sugar-phosphate nucleotidyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313E05C", "UniRef:UniRef90_UPI001313E05C" ], "nt": "TTGGGCTATAAAGTAATATATTCGCATATTGATAATGGGCAAGATTTTTATAAATATGAGCCTATTGAAAATTATGATTTAATAATATCGAATCCTCCGTTTAGAAATAAAGCAAATATTATTAAAAGATTGCAAGAACTAAATAAACCGTTTGCTTTAATTTTTGGAGTTCAATGTTTTAATTCTGGCGGTTTTGTAAGCCAATTGCAAAAACTAAAAAATTTAGAGCTTGTGTTCTTAACAAAAAGAATTAAATTTCTTAAAAATTATAAACAAGACCTTAAAAACATTCCTCAGCCTACTTTTCATTCTCTATGA", "aa": "MGYKVIYSHIDNGQDFYKYEPIENYDLIISNPPFRNKANIIKRLQELNKPFALIFGVQCFNSGGFVSQLQKLKNLELVFLTKRIKFLKNYKQDLKNIPQPTFHSL", "aa_hexdigest": "3b68e2eb6f24447bacae676d47247993", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 757945, "stop": 758361, "observations": { "mutation": [ 758305 ], "stop": [ 758305 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001313E05C", "query_cov": 1.0, "subject_cov": 0.7553956834532374, "identity": 0.99, "score": 214.0, "evalue": 2.72e-70, "gene_start": 1, "gene_end": 105, "reference_start": 17, "reference_end": 121, "reference_sequence": "MPFDTQQSNFVIVLKSLGYKVIYSHIDNGQDFYKYEPIENYDLIISNPPFRNKANIIKRLQELNKPFALIFGVQCFNSGGFVSQLQKLKNLELVFLTKRIKFLKNYKQDLKNIPQPTFHSLWICSGITNKPLSILEGVK" }, "paralog": false, "identity": 0.9855072463768116, "score": 276.0, "evalue": 5.18e-97, "description": "Internal stop codon at 758305. Nonsense mutation around 758305.", "psc": { "uniref90_id": "UniRef90_UPI001313E05C", "product": "sugar-phosphate nucleotidyltransferase", "uniref50_id": "UniRef50_UPI001313E05C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001313E05C", "UniRef:UniRef50_UPI001313E05C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001313E05C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313E05C" ], "product": "sugar-phosphate nucleotidyltransferase" } }, "id": "DFOKDJOJIG_1337", "locus": "GCA013367855_01336" }, { "type": "cds", "sequence": "contig_1", "start": 758886, "stop": 759143, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATAAAAATAATCCTAATCCTACCCCAACACCGGGTCCAGATTCGGGTTTAGTAAATCCAAATCCTTCAAACCCAACTCCTGCTCCTGCTCCAGTTCCAACCCCTGAAGTTAAACCAGAAGAAAAAGAAGGTTATGTTATTTATAAATCGACAAATGAAGATTATTTAAAAAAGAATAACACTTATTATTTTAGCAAATTGAGTTATAACCCCGTGGAATTGTCTGATGAGATTTATAATAGTCAAAAATGA", "aa": "MINKNNPNPTPTPGPDSGLVNPNPSNPTPAPAPVPTPEVKPEEKEGYVIYKSTNEDYLKKNNTYYFSKLSYNPVELSDEIYNSQK", "aa_hexdigest": "a91a8a19ef47d6e17cbb43d043924e66", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9484.356899999999, "isoelectric_point": 4.962578392028808 }, "id": "DFOKDJOJIG_1338", "locus": "GCA013367855_01337" }, { "type": "cds", "sequence": "contig_1", "start": 759315, "stop": 759521, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGAAAGTGATGAAAACAAAGAAGGTATCAACCAAGGCGGTCGTTTTAAACCAAAAGATAATGAAAGTTATAATGAAGCCTTAATGAGATTTTTAAAAGAAATGGATGATTATTTAGATCCTGATATTGCATTTAAAGGTTTAACAATGGATGAAACTCATATAAATTATGTAACGGAAAATTTAGCTAAAGATCCTAAATGA", "aa": "MAESDENKEGINQGGRFKPKDNESYNEALMRFLKEMDDYLDPDIAFKGLTMDETHINYVTENLAKDPK", "aa_hexdigest": "e8234ce9e96894d500c75ddc58d5bb15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7872.6335999999965, "isoelectric_point": 4.491837882995605 }, "id": "DFOKDJOJIG_1339", "locus": "GCA013367855_01338" }, { "type": "cds", "sequence": "contig_1", "start": 759558, "stop": 759668, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E37B992", "UniRef:UniRef90_UPI001E37B992" ], "nt": "ATGCCTTCTACTTTTATTAGAGACGAATTTAACCACCAATCAATTGAAGACATGGATGGATTTGTTAAAGAATTAATATCTGATTATGAAAATGCTATGTATGAAGGATGA", "aa": "MPSTFIRDEFNHQSIEDMDGFVKELISDYENAMYEG", "aa_hexdigest": "ca841bba6d4befa1bd0c027b5776d996", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 759531, "stop": 759789, "observations": { "insertion": [ 759708 ], "mutation": [ 759666 ], "stop": [ 759666, 759733 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E37B992", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 1.0, "score": 76.6, "evalue": 1.6e-17, "gene_start": 1, "gene_end": 36, "reference_start": 12, "reference_end": 47, "reference_sequence": "MNKNLFTHDSKMPSTFIRDEFNHQSIEDMDGFVKELISDYENAMYEGWTVENKANDWFVAPFHLDLILQWEINFHNKLMICILIKMV" }, "paralog": false, "identity": 0.9176470588235294, "score": 155.0, "evalue": 5.39e-51, "description": "Internal stop codon at 759666, 759733. Frameshift due to insertion around 759708. Nonsense mutation around 759666.", "psc": { "uniref90_id": "UniRef90_UPI001E37B992", "uniref50_id": "UniRef50_UPI001E37B992", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E37B992", "UniRef:UniRef50_UPI001E37B992" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E37B992", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E37B992" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4259.6153, "isoelectric_point": 4.0500284194946286 }, "id": "DFOKDJOJIG_1340", "locus": "GCA013367855_01339" }, { "type": "cds", "sequence": "contig_1", "start": 759732, "stop": 759914, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAATAAATTTCCACAACAAACTTATGATATGTATACTGATAAAAATGGTCTAGATAAAACAACTCCTACTCCTGCTTATAATAACTACAATCCAGTAGCATTTAATTTCTTAGTAATGGAAAGATTTGACTTATCAGAAAAATTAAATTTTAAAGTTTATAAGAATTTAATTCAATGA", "aa": "MRNKFPQQTYDMYTDKNGLDKTTPTPAYNNYNPVAFNFLVMERFDLSEKLNFKVYKNLIQ", "aa_hexdigest": "35ddc5bf27f61669d63858c8582cfd40", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7217.132799999999, "isoelectric_point": 9.003735160827638 }, "id": "DFOKDJOJIG_1341", "locus": "GCA013367855_01340" }, { "type": "cds", "sequence": "contig_1", "start": 760113, "stop": 760220, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E44B7D1", "UniRef:UniRef90_UPI001E44B7D1" ], "nt": "ATGAAGAGTTTAACAATAGGTGACAAAATTGACTTTGATTACACATATAGATTTGCTTATAAATTTTTATATGAAAAATTAGTGCCTACAATGGTGGCAAACGGTTGA", "aa": "MKSLTIGDKIDFDYTYRFAYKFLYEKLVPTMVANG", "aa_hexdigest": "8e5b417499fbc7ff4464986ab040ae0f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 760113, "stop": 760268, "observations": { "mutation": [ 760218 ], "stop": [ 760218 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E44B7D1", "query_cov": 1.0, "subject_cov": 0.660377358490566, "identity": 0.971, "score": 72.8, "evalue": 2.16e-16, "gene_start": 1, "gene_end": 35, "reference_start": 1, "reference_end": 35, "reference_sequence": "MKSLTMGDKIDFDYTYRFAYKFLYEKLVPTMVANGWNMPGEFDVLEKNMLDEK" }, "paralog": false, "identity": 0.8627450980392157, "score": 91.7, "evalue": 1.3e-26, "description": "Internal stop codon at 760218. Nonsense mutation around 760218.", "psc": { "uniref90_id": "UniRef90_UPI001E44B7D1", "uniref50_id": "UniRef50_UPI001E44B7D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E44B7D1", "UniRef:UniRef50_UPI001E44B7D1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E44B7D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E44B7D1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4139.787800000002, "isoelectric_point": 8.032776832580566 }, "id": "DFOKDJOJIG_1342", "locus": "GCA013367855_01341" }, { "type": "cds", "sequence": "contig_1", "start": 760224, "stop": 760400, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATTGAATTTAATATTTTAGAAACGCTTATGCTAGACAGAAATAAAAAAGATTCATATATTTATGACTATATCTTTAAATATATAAATGTCATTTTTAAAGCTAAATATAAAGACATTGATGAAATCAAAAAACCAAATGGATATTTAGATTTAAATAATAAAGAACATTGA", "aa": "MPIEFNILETLMLDRNKKDSYIYDYIFKYINVIFKAKYKDIDEIKKPNGYLDLNNKEH", "aa_hexdigest": "fc965a11b161dd76f65b83aeaaa726d5", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7120.160200000002, "isoelectric_point": 6.546112632751465 }, "id": "DFOKDJOJIG_1343", "locus": "GCA013367855_01342" }, { "type": "cds", "sequence": "contig_1", "start": 760620, "stop": 760739, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATCGATGATAAAAGCTTTAGCTTTTTAGGAAATTTTAGTGAAACTATAATGAGGTCTATTTTTATAGCCCTTATTCCAGTTGCAATTATTGTATTAATAGGAATGATATTGTGA", "aa": "MKIDDKSFSFLGNFSETIMRSIFIALIPVAIIVLIGMIL", "aa_hexdigest": "d18ca949acc3f7ed5bd5f985436aca2a", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4358.317699999999, "isoelectric_point": 5.876663017272949 }, "id": "DFOKDJOJIG_1344", "locus": "GCA013367855_01343" }, { "type": "cds", "sequence": "contig_1", "start": 760984, "stop": 761208, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E52DBAB", "UniRef:UniRef90_UPI001E52DBAB" ], "nt": "ATGAAATTTTTACAGGCGATAATAAATCCTTTAATAAATGTAATCGCAGCGCCTATTAGGCTTATGTGGTTTGTACCTCTTGCTGCTATTGGATTAATAGGATTTACTATATTTAATCTTTTGTTTTTCTTGTTTAAATTGTTTAGCCTCGATTTAAACAACTATTTAATGTTTAATTTATTACCAGGCCAAAGTTATAGCCAATTTTCTGTAGCAAGGCCTTGA", "aa": "MKFLQAIINPLINVIAAPIRLMWFVPLAAIGLIGFTIFNLLFFLFKLFSLDLNNYLMFNLLPGQSYSQFSVARP", "aa_hexdigest": "d1e23a59454a415dd491de6565c094cc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 760984, "stop": 761296, "observations": { "insertion": [ 761251 ], "mutation": [ 761206, 761239 ], "stop": [ 761206, 761239 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E52DBAB", "query_cov": 1.0, "subject_cov": 0.7184466019417476, "identity": 0.973, "score": 135.0, "evalue": 8.74e-40, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MGFLKAIINPLINVIAAPIRLMWFVPLAAIGLIGFTIFNLLFFLFKLFSLDLNNYLMFNLLPGQSYSQFSVARPWYQFLIISFILWLINFLCLLLGIQKNLMD" }, "paralog": false, "identity": 0.941747572815534, "score": 182.0, "evalue": 3.87e-61, "description": "Internal stop codon at 761206, 761239. Frameshift due to insertion around 761251. Nonsense mutation around 761206, 761239.", "psc": { "uniref90_id": "UniRef90_UPI001E52DBAB", "uniref50_id": "UniRef50_UPI001E52DBAB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E52DBAB", "UniRef:UniRef50_UPI001E52DBAB" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E52DBAB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E52DBAB" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8468.174799999993, "isoelectric_point": 9.991971015930176 }, "id": "DFOKDJOJIG_1345", "locus": "GCA013367855_01344" }, { "type": "cds", "sequence": "contig_1", "start": 761901, "stop": 762431, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTATTCTTTCGATTTGGCATTATTAAAATAATTCCTAATAAAAAAGTAGGTATTCAAAATGCAAAGAAAATTAATTTCACATTTATTAATTGGCTAAAACCAAAACTAATAAAAAATGATAATAATAGAATTACAACAAATTCAATAAGATCTTTTGTTTTGAAAGTTTTTCAAATTTTACTACTTCTATGTTTTATTCTACTTGGCTGTAATTGCGTCATATTACTCCTTATACTTTGCTATTTTAGAAAACTTGCAACTTTATATGCTGCACCTACATTCGTGCCCTTTAATGCTGCATCCTTTACTGTTTTGTATGCTCCTGCCCCTGATCTTGCTCCAGACATAGCAAGTCTTGCAGATTGGCCACCAGAAACTTCACTTCCAACAAAAGCAATTGCAATTTCTGATAATTTTTTGAAAGCAAATGCAGAGCCAATTACCAAGAAACAAGTTGATAATAATGCTAAAACGGCTTTGATAGTAGTAAAGTTTGAGAATGAAGAAAAGGCTCCTATCATATAA", "aa": "MQLFFRFGIIKIIPNKKVGIQNAKKINFTFINWLKPKLIKNDNNRITTNSIRSFVLKVFQILLLLCFILLGCNCVILLLILCYFRKLATLYAAPTFVPFNAASFTVLYAPAPDLAPDIASLADWPPETSLPTKAIAISDNFLKANAEPITKKQVDNNAKTALIVVKFENEEKAPII", "aa_hexdigest": "602567f04168dc9dd7e9ce7b340f50b7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19755.3747, "isoelectric_point": 9.608642005920409 }, "id": "DFOKDJOJIG_1346", "locus": "GCA013367855_01345" }, { "type": "cds", "sequence": "contig_1", "start": 762523, "stop": 762660, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTAAATCCTTATTCATGTTTAGTTAATGAATATGTAGTCAAAAATAAAAATTCAAAAATAAGTTTTTATAATCCAAGAAATGATAAAGAAAACTATTTAGCGGTATTTAAACTAAATGGTCTTAATATTTGA", "aa": "MDLNPYSCLVNEYVVKNKNSKISFYNPRNDKENYLAVFKLNGLNI", "aa_hexdigest": "e4028ef62a76857cd24bf8df97f42e76", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5283.9867, "isoelectric_point": 8.852041053771973 }, "id": "DFOKDJOJIG_1347", "locus": "GCA013367855_01346" }, { "type": "cds", "sequence": "contig_1", "start": 762997, "stop": 763233, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATAAAGCAAGACTTCTAACATTTCTTAATAAACTTAATATGTTTGGTATTGATAGCCAAAACATACAAGAATATTGCAATGATTTAGATGCTAAATTTGGAGTTGAAAATTCATTAGATAACTTATTTATTTATAATCAAATTATTATTAAAGATAAATATTTACAAATCAATGATAATTACGTAAAAATCAATTGTTTAAACAAATTGCCATTTGAATTGTCTGATAAATGA", "aa": "MHKARLLTFLNKLNMFGIDSQNIQEYCNDLDAKFGVENSLDNLFIYNQIIIKDKYLQINDNYVKINCLNKLPFELSDK", "aa_hexdigest": "bb48840de9856d6d1d99c6de438389c9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9218.523299999993, "isoelectric_point": 5.646124076843263 }, "id": "DFOKDJOJIG_1348", "locus": "GCA013367855_01347" }, { "type": "cds", "sequence": "contig_1", "start": 763327, "stop": 764049, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01656.29", "PFAM:PF01935.23", "PFAM:PF10412.15" ], "nt": "ATGGCATCTATTGATACTACTAACGCAACAGGATCGGTGTCAGCAAACTTGGTTGGTTCTATTGATGAACAAGCGACAATGGCGATGATGTATCAAGTAAATAAAGATGAATACAAACTATTTGATAGTAATTTCTTCTTTGTATCAAAAGCCACTGACCTTGCTGAATTAAATAGACAGCATAGAATTGCTAGAAATATAGCAAGTAAAAAGAATATAAGCTTAAATGACTTAAAATATAGACAGCTTTTGGGTTTATCTGAGGCTTGCAATTATCCTTTTGAAAGATTAGAAAGAGAATATTATCAAATTACATCTATTAATTTAGCTTTGGGATGGCCTTTTGAAACCGAAAACCACAATGATTCAAATAATTTACTTCTAGGTTACTCAGAAGCCACAAGAAGTCCTCTAAGTTTTAGATTATTTGATATGTATCATTCAAATAGAACTAATTTTAATATGTTTATAAATGGTACTAGTGGAAAAGGTAAAACAACTTTTACTATGAAGTTATTAACCAGCATTTTAGCAGCAAATAATAAAGTTGTTGTTATTGACCCTCAAGGAGAATATAGAGAATTAGCTCAAAAACTTAATGGACAAATTATTGATTTGGGAGCAGGTAAAAACACCATAATCAATCCTCTTCAAATCCGTAATTCATTAAAGGATGCAACGGGTAATGATACATCTTCACTAATTAACAACCATTTGTCGTGA", "aa": "MASIDTTNATGSVSANLVGSIDEQATMAMMYQVNKDEYKLFDSNFFFVSKATDLAELNRQHRIARNIASKKNISLNDLKYRQLLGLSEACNYPFERLEREYYQITSINLALGWPFETENHNDSNNLLLGYSEATRSPLSFRLFDMYHSNRTNFNMFINGTSGKGKTTFTMKLLTSILAANNKVVVIDPQGEYRELAQKLNGQIIDLGAGKNTIINPLQIRNSLKDATGNDTSSLINNHLS", "aa_hexdigest": "cf0d37f42301b4c3b8415fe707399eb9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01656.29", "name": "CbiA", "length": 42, "aa_cov": 0.175, "hmm_cov": 1.0, "evalue": 2.9843715594778944e-07, "score": 26.120105743408203, "start": 155, "stop": 196 }, { "id": "PF01935.23", "name": "DUF87", "length": 68, "aa_cov": 0.26666666666666666, "hmm_cov": 0.9705882352941176, "evalue": 2.462838157755879e-10, "score": 36.4718132019043, "start": 157, "stop": 220 }, { "id": "PF10412.15", "name": "TrwB_AAD_bind", "length": 72, "aa_cov": 0.3, "hmm_cov": 0.875, "evalue": 1.5735730484289072e-10, "score": 36.0557746887207, "start": 149, "stop": 220 } ], "seq_stats": { "molecular_weight": 26953.050900000024, "isoelectric_point": 6.912837791442871 }, "id": "DFOKDJOJIG_1349", "locus": "GCA013367855_01348" }, { "type": "cds", "sequence": "contig_1", "start": 764101, "stop": 764478, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTCAAAACGTAATAAAAGAATTTTATGAAGATAGAGGAATATATAAACTTGCTTCTATTGAGCAATTAAAGGCCGTAGAATGGCCAACAATAAGTGAGTTGATAAAATATATGTCCAACTATAAAACGCTTCATTTAAACAATCCTGAAGCAAAAAGAGCAATATTAGTTGAACTTTGTGAATCGTTTAGCTTTTTGTTTGAAAATAATGGAAGATATCAAAACTTATTTAATGGACATACTAATATAAAACTTGATAATGATTTTGTAGTATTTAATACGCAAAACTTAATAAATGGAGCGGATAATATCGGCGCTAAATTAGGAACAATGTGCTTATTAAACTTTGTTAGTGAAATAGTATTTAATAATTGA", "aa": "MLQNVIKEFYEDRGIYKLASIEQLKAVEWPTISELIKYMSNYKTLHLNNPEAKRAILVELCESFSFLFENNGRYQNLFNGHTNIKLDNDFVVFNTQNLINGADNIGAKLGTMCLLNFVSEIVFNN", "aa_hexdigest": "b7282ea7acc765500ab8863ad00101c8", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14430.339799999983, "isoelectric_point": 5.328053092956543 }, "id": "DFOKDJOJIG_1350", "locus": "GCA013367855_01349" }, { "type": "cds", "sequence": "contig_1", "start": 764491, "stop": 764982, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F9", "UniRef:UniRef90_A0A454C9F9" ], "nt": "ATGTTAAAGAGAAAATATTTGCAAGAACATAACATTAAGTTAATGGATGCAAAACAAGTAGAAGATACTATTAGATTTTGTGCCGTCATCGTAGATGAAGCCCATTTATACATCGACCCTGATAATCCTGCCATTTTAAAATATATGGTTCAAATGACAAAAACGGTTAGAAAATTCAATGCAGGTATGATATTTACTACCCAAAATCCTGGTGATTTTACTATTAATAATGCCACAAGTGATGATGCATCTAGAATTATTCAAAACTGTCAATATTCTGTATTCTTTGGCCTAAAAGACCAAGATATTGACAAAGTGCAACAATTATTTAAAAACTCTAATCAATTATTACAAAGTGAAATTAGATATTTAGTAAATGCAAGATATGGAAAATGTTTATTTTCAATAAGTTCAAATAAAAGAATTAGAGTTGAAATTTATTACAACAAATTAGAACAAGAATTATTCTTCAAGTTCTTAAATAAAGAATGA", "aa": "MLKRKYLQEHNIKLMDAKQVEDTIRFCAVIVDEAHLYIDPDNPAILKYMVQMTKTVRKFNAGMIFTTQNPGDFTINNATSDDASRIIQNCQYSVFFGLKDQDIDKVQQLFKNSNQLLQSEIRYLVNARYGKCLFSISSNKRIRVEIYYNKLEQELFFKFLNKE", "aa_hexdigest": "dc888545ecc1107b7cb41b23ec2f7010", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 764191, "stop": 764982, "observations": { "mutation": [ 764476 ], "stop": [ 764476 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9F9", "query_cov": 1.0, "subject_cov": 0.5174603174603175, "identity": 0.9940000000000001, "score": 323.0, "evalue": 8.79e-110, "gene_start": 1, "gene_end": 163, "reference_start": 151, "reference_end": 313, "reference_sequence": "MSWLESFFKVLINIEDHKWILLQNVIKEFYEDRGIYKLASIEQLKAVEWPTISELIKYMSNYKTLHLNNPEAKRAILVELCESFSFLFENNGRYQNLFNGHTNIKLDNDFVVFNTQNLINGADNIGAKLGTMCLLNFVSEIVFNNWTHNEMLKRKYLQEHNIKLMDAKQVEDTIRFCAVIVDEAHLYIDPDNPAILKYMVQMTKTVRKFNAGMIFTTQNPGDFTVNNATSDDASRIIQNCQYSVFFGLKDQDIDKVQQLFKNSNQLLQSEIRYLVNARYGKCLFSISSNKRIRVEIYYNKLEQELFFKFLNKEWI" }, "paralog": false, "identity": 0.9923954372623575, "score": 526.0, "evalue": 3.22e-192, "description": "Internal stop codon at 764476. Nonsense mutation around 764476.", "psc": { "uniref90_id": "UniRef90_A0A454C9F9", "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A454C9F9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9F9", "UniRef:UniRef50_A0A454C9F9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9F9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F9" ], "product": "ATP-binding protein" } }, "id": "DFOKDJOJIG_1351", "locus": "GCA013367855_01350" }, { "type": "cds", "sequence": "contig_1", "start": 765040, "stop": 765306, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAACTAATAAAAACGAATTAGATCAAGATTTTGATAATTTAGATAATAATGAAATTTTATCAAATGACAATCAAACTCAAAATGAAGATGACTTTATAGAAAATATTAATTTAACAAGTGATAATCAAATAAATAACATTATGAATTCTATCACTAGTTCCCAACCTAAAAAGCAAAAGGAAAAAAAGCAACAAAACAATTTTGAAGCACAATTTTCAGGACGATTTACTGATCCTGAAGATGTAGGAATGTTTTATCGTTGA", "aa": "MKTNKNELDQDFDNLDNNEILSNDNQTQNEDDFIENINLTSDNQINNIMNSITSSQPKKQKEKKQQNNFEAQFSGRFTDPEDVGMFYR", "aa_hexdigest": "99fc51e43057f7e6bac09ce1ee956bb5", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10338.974399999996, "isoelectric_point": 4.283920860290528 }, "id": "DFOKDJOJIG_1352", "locus": "GCA013367855_01351" }, { "type": "cds", "sequence": "contig_1", "start": 765511, "stop": 765675, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00138A25BD", "UniRef:UniRef90_UPI00138A25BD" ], "nt": "ATGTCTATTATTAATTTAAAACTTAATATGATTATTAATGCTTTATTTAAAGATAATGGAGATTTAGAAAAACAAATTGATAATCCCGGGGCTGATATATTAGCAGAATCTAAGTTCTTTGAACGCACTATAAGAGGTGCTGTTAATAAATTAACTAATGATTGA", "aa": "MSIINLKLNMIINALFKDNGDLEKQIDNPGADILAESKFFERTIRGAVNKLTND", "aa_hexdigest": "6426191af12777e3dadae3b37578ec71", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 765451, "stop": 765684, "observations": { "mutation": [ 765673 ], "stop": [ 765673 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00138A25BD", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 110.0, "evalue": 1.14e-30, "gene_start": 1, "gene_end": 54, "reference_start": 22, "reference_end": 75, "reference_sequence": "MSALSPYQLQINRRFRSMDIEMSIINLKLNMIINALFKDNGDLEKQIDNPGADILAESKFFERTIRGAVNKLTNDWTKNGK" }, "paralog": false, "identity": 0.987012987012987, "score": 149.0, "evalue": 2.08e-48, "description": "Internal stop codon at 765673. Nonsense mutation around 765673.", "psc": { "uniref90_id": "UniRef90_UPI00138A25BD", "uniref50_id": "UniRef50_UPI00138A25BD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00138A25BD", "UniRef:UniRef50_UPI00138A25BD" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00138A25BD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00138A25BD" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6063.910899999999, "isoelectric_point": 5.175554084777831 }, "id": "DFOKDJOJIG_1353", "locus": "GCA013367855_01352" }, { "type": "cds", "sequence": "contig_1", "start": 765952, "stop": 766269, "strand": "+", "frame": 1, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA66", "UniRef:UniRef90_A0A454CA66" ], "nt": "TTGCCCTTTGTTATTGCACTATTTGAACAAGCAAATAAAACCAATTTTATCGAGATTACTTGTTTTAATAAATTGGCAGAACATGTTATGAAATATGTTAAAAAAGGAGATTTATTAGAAGTTGTTGGCAAATTACACAATGCTAAATTTAGTTCAAAGAATAACGAAACTATTTATAAAACAGAAATAATTGCTAAAAATATTACTTTTTTGACAAAGTCAAAATCAAATATTCAACCCATTTCAAAAGAAACCAACTCAGAAAATAATGAAAAAGACATTTTTGGAATAGATTTTTTTGAAGATGAAGAAAATTAA", "aa": "MPFVIALFEQANKTNFIEITCFNKLAEHVMKYVKKGDLLEVVGKLHNAKFSSKNNETIYKTEIIAKNITFLTKSKSNIQPISKETNSENNEKDIFGIDFFEDEEN", "aa_hexdigest": "f16d7948cf267e4824cd45f63ab496fc", "start_type": "TTG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A454CA66", "query_cov": 1.0, "subject_cov": 0.8076923076923077, "identity": 0.99, "score": 201.0, "evalue": 6.25e-65, "valid": true, "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_A0A454CA66" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA66", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA66" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "DFOKDJOJIG_1354", "locus": "GCA013367855_01353" }, { "type": "cds", "sequence": "contig_1", "start": 766256, "stop": 766450, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EDE39", "UniRef:UniRef90_UPI001E2EDE39" ], "nt": "ATGAAGAAAATTAAAAAAACCTTATTGATAGCCGGAGGAATATTTAGTATTGCTTCGGCTTCTCTATTTTCTTTGAGTTTTTCTAGAAATGAACATTATTCAAGTTATAAATATGAAGAAATTGACAAAGACCAATATTCTTTTTTAAACAACAAAAATTTATTAAAAAGTTTTTTTACAAATTTGAGATATTGA", "aa": "MKKIKKTLLIAGGIFSIASASLFSLSFSRNEHYSSYKYEEIDKDQYSFLNNKNLLKSFFTNLRY", "aa_hexdigest": "74cf0149fcb0adb2e478ce6d8c47e11d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 766256, "stop": 766550, "observations": { "insertion": [ 766517 ], "mutation": [ 766448 ], "stop": [ 766448 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2EDE39", "query_cov": 1.0, "subject_cov": 0.6530612244897959, "identity": 0.9840000000000001, "score": 121.0, "evalue": 1.07e-34, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MKKIKKTLLIAGGIFSIASASLFSLSFSRNEHYSSYKYEEIDKDQYSFLNNKNLLKSFFTNLTYKKNSRQDSFIFNPKSYSGNPLYSIKDLLQTKMIT" }, "paralog": false, "identity": 0.9175257731958762, "score": 168.0, "evalue": 9.44e-56, "description": "Internal stop codon at 766448. Frameshift due to insertion around 766517. Nonsense mutation around 766448.", "psc": { "uniref90_id": "UniRef90_UPI001E2EDE39", "uniref50_id": "UniRef50_UPI001E2EDE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2EDE39", "UniRef:UniRef50_UPI001E2EDE39" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2EDE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EDE39" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7510.534099999998, "isoelectric_point": 9.646807289123537 }, "id": "DFOKDJOJIG_1355", "locus": "GCA013367855_01354" }, { "type": "cds", "sequence": "contig_1", "start": 766691, "stop": 766840, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGGCTACAAAAAATATATCAACGACTTAACTTTTTATCACGGCGATGGTAATGAGTGGTGTGATCAAGATGGGTGCCAGGAAGAAACACATAGCAACGGCGAAATTACTCAAACTATTAAAAAATCTAATTATAGTATTAACTGA", "aa": "MYGYKKYINDLTFYHGDGNEWCDQDGCQEETHSNGEITQTIKKSNYSIN", "aa_hexdigest": "9ceab7927a4c10fe9c661039847e1820", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5740.112799999999, "isoelectric_point": 4.734767723083496 }, "id": "DFOKDJOJIG_1356", "locus": "GCA013367855_01355" }, { "type": "cds", "sequence": "contig_1", "start": 767114, "stop": 767611, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGCATTTCATCTACAAAATTCAAGTCAAGAAATTATTATAGATTCTGATACTATGGGAAAATTAACGACAGGTATTGATTTAAATATTTTTAAAGGTGGTAAAAGTAATCTTGAAAAACTACAAGAAATTGTTGAACAAATGGATAATGAAACAGCAATTGGCAATTTTATTATTAAATATGGTTATGAAGTCAAACCTATTTCAGATGGAAATCATTTTAATCTAATTTTTAAAATCAAACAATTAAAATATAAGGGAAAAGATATCACTAATTCTTTTGCTTCTAAAATTTTTAATGAAGCAGATACTTATAACTTTGCTAATAATTTTGCCAAAAATCAACAAAGTAAAATTCAAATTAAGAATCTTTATCATATGTTTTATAGTCTTTTTAAAGGCAAGACAATAATGGCTAATGTTCCAGTAAGATTTACTCCAAGCCAAATTTATCAAACTTATGCAGACAAGACAGGATTTGTTAGTAGATTATGA", "aa": "MSAFHLQNSSQEIIIDSDTMGKLTTGIDLNIFKGGKSNLEKLQEIVEQMDNETAIGNFIIKYGYEVKPISDGNHFNLIFKIKQLKYKGKDITNSFASKIFNEADTYNFANNFAKNQQSKIQIKNLYHMFYSLFKGKTIMANVPVRFTPSQIYQTYADKTGFVSRL", "aa_hexdigest": "18837d5f2cab88a1db467aa3e85a243a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18891.375399999983, "isoelectric_point": 9.260125923156735 }, "id": "DFOKDJOJIG_1357", "locus": "GCA013367855_01356" }, { "type": "cds", "sequence": "contig_1", "start": 767753, "stop": 768100, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATACTCCACTACAAGTAAATTTTGACACCACATTAGAAGAAAATGAAGTTTTATTTATAAATGGTCAAAAAGTTGATGTTTTAAATAGACATTTTGTTTATCATTTAAAAGATTTAAGACGTTCGGCATCCGATGAAGAAAAAATAGCATTAGAGAAAAATGATTCAAATAAGCCTCAAGAAGAGTTAAATGACGATAATTCAAGAAAGAAAAACGAGTATATAGTTGAAATAAAGAAATTCAAACCTGGCACAAATAATAGCGGAGAGCCAATAGCAACTTATAAAATTAAGTTCGTTATAGTTGAACAACCAATCAACCAATCATTTAAATGGTTTGCTTGA", "aa": "MHTPLQVNFDTTLEENEVLFINGQKVDVLNRHFVYHLKDLRRSASDEEKIALEKNDSNKPQEELNDDNSRKKNEYIVEIKKFKPGTNNSGEPIATYKIKFVIVEQPINQSFKWFA", "aa_hexdigest": "8533c2db2702be0af9ebac919fac8f16", "start_type": "GTG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13495.009399999984, "isoelectric_point": 5.925374031066894 }, "id": "DFOKDJOJIG_1358", "locus": "GCA013367855_01357" }, { "type": "cds", "sequence": "contig_1", "start": 768323, "stop": 768556, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAATGATTTTGGTATTTTTGCAGAGGGCGCTGTCCTTGGTAAAGGAGCCTTAAGAAATCTAATCTTAGATAAAAATATTGCCAAAAAATCTAATGTTACATACTATAAATGCAAAATTTATGATAAAGGAAGCAACAAATGGCTTAGTCCTAGTGAAATTAAACCTGAACCATTATCTTCATCAAACGGTAATAGTGAAAATGCTTATATGTCTGAAGAAGGCATCTGA", "aa": "MLNDFGIFAEGAVLGKGALRNLILDKNIAKKSNVTYYKCKIYDKGSNKWLSPSEIKPEPLSSSNGNSENAYMSEEGI", "aa_hexdigest": "b45baf31aa37607b88b8d8fbcdf77cef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8459.5085, "isoelectric_point": 7.798563194274903 }, "id": "DFOKDJOJIG_1359", "locus": "GCA013367855_01358" }, { "type": "cds", "sequence": "contig_1", "start": 768983, "stop": 769207, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823", "UniRef:UniRef90_UPI001314F823" ], "nt": "ATGTCAAGCAACACATTAACCAAAAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACCGAGGAAAATAAATTTCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGA", "aa": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEA", "aa_hexdigest": "4cf89e55fbd43bc2c9828114fb6c5739", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 768983, "stop": 769433, "observations": { "insertion": [ 769394 ], "mutation": [ 769205 ], "stop": [ 769205 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314F823", "query_cov": 1.0, "subject_cov": 0.4966442953020134, "identity": 1.0, "score": 145.0, "evalue": 1.93e-43, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEAWHVYNPPIDLSTLDIQNEFILNISKKTFEEKNIPEDVVDERYRKAIEKEVINFITNQLLQYQLKQNLLAKKLKCY" }, "paralog": false, "identity": 0.9798657718120806, "score": 287.0, "evalue": 1.32e-101, "description": "Internal stop codon at 769205. Frameshift due to insertion around 769394. Nonsense mutation around 769205.", "psc": { "uniref90_id": "UniRef90_UPI001314F823", "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314F823", "UniRef:UniRef50_UPI001314F823" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8600.459199999998, "isoelectric_point": 4.854925346374511 }, "id": "DFOKDJOJIG_1360", "locus": "GCA013367855_01359" }, { "type": "cds", "sequence": "contig_1", "start": 769241, "stop": 769768, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCAGAAGATGTTATTGATGAAAGATATAGAAAAGCAATAGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCCAATTTAAATGAAGTAATTGCCCATTTAATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTCTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGA", "aa": "MDIQSEFILNISKKTFEEKNIPEDVIDERYRKAIEKEVINFITNQLLQYQLKTKSSGKEIEMLLNKDVEIANLNEVIAHLMRGKALSSFVGLKLVSKNANLHNYKIINFKNLGEGGKFNLRNLSYILMDNKNVIQETKPSQIRKKVIEIVVNAASKLGLEINNDYQIFAINNDAY", "aa_hexdigest": "16cb6b67b60f78684bdb9d0db65ba5e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20119.024600000004, "isoelectric_point": 8.923278617858887 }, "id": "DFOKDJOJIG_1361", "locus": "GCA013367855_01360" }, { "type": "cds", "sequence": "contig_1", "start": 769769, "stop": 770122, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358", "UniRef:UniRef50_UPI0027330C60", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef90_UPI001314B421" ], "nt": "TTGGAATTAGTTTCGAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAGGCTCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTCGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATATAAGAAAATTCTTTTATTACCTACAAACAAAACCACCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATAGACCCTGATTGA", "aa": "MELVSKYDDPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTTGFGYGYIINKANKEYNPAIDPD", "aa_hexdigest": "7338646e778a5dfe217af27c3d57dad9", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001314B421", "query_cov": 0.8632478632478633, "subject_cov": 0.9805825242718447, "identity": 0.96, "score": 179.0, "evalue": 1.05e-56, "valid": true, "uniref50_id": "UniRef50_UPI0027330C60" }, "pscc": { "uniref50_id": "UniRef50_UPI0027330C60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027330C60" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 769793, "stop": 770155, "observations": { "mutation": [ 770120 ], "stop": [ 770120 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005C90358", "query_cov": 0.9316239316239316, "subject_cov": 0.4448979591836735, "identity": 0.982, "score": 205.0, "evalue": 6.14e-65, "gene_start": 9, "gene_end": 117, "reference_start": 1, "reference_end": 109, "reference_sequence": "NPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPDWKPNPNPDDEDIVSPEDIPKDPNRIKKVVKTWWFPLVVIVGILTFLVASIFIIKSLLRKYGWSFGKRAKRIRRQNPNKQSYQEKRSNKRQEKLAKKEAKNLKRQTKKQEKANFIQAKKDKKTKNKSKTNNEQNSIN" }, "paralog": false, "identity": 0.975, "score": 239.0, "evalue": 6.85e-81, "description": "Internal stop codon at 770120. Nonsense mutation around 770120.", "psc": { "uniref90_id": "UniRef90_UPI0005C90358", "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef50_UPI0005C90358" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13926.73179999998, "isoelectric_point": 5.6632894515991214 }, "id": "DFOKDJOJIG_1362", "locus": "GCA013367855_01361" }, { "type": "cds", "sequence": "contig_1", "start": 770264, "stop": 770488, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823", "UniRef:UniRef90_UPI001314F823" ], "nt": "ATGTCAAGCAACACATTAACCAAAAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACTGAGGAAAATAAATTCCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGA", "aa": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEA", "aa_hexdigest": "4cf89e55fbd43bc2c9828114fb6c5739", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 770264, "stop": 770714, "observations": { "insertion": [ 770675 ], "mutation": [ 770486 ], "stop": [ 770486 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314F823", "query_cov": 1.0, "subject_cov": 0.4966442953020134, "identity": 1.0, "score": 145.0, "evalue": 1.93e-43, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEAWHVYNPPIDLSTLDIQNEFILNISKKTFEEKNIPEDVVDERYRKAIEKEVINFITNQLLQYQLKQNLLAKKLKCY" }, "paralog": false, "identity": 0.9798657718120806, "score": 287.0, "evalue": 1.32e-101, "description": "Internal stop codon at 770486. Frameshift due to insertion around 770675. Nonsense mutation around 770486.", "psc": { "uniref90_id": "UniRef90_UPI001314F823", "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314F823", "UniRef:UniRef50_UPI001314F823" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8600.459199999998, "isoelectric_point": 4.854925346374511 }, "id": "DFOKDJOJIG_1363", "locus": "GCA013367855_01362" }, { "type": "cds", "sequence": "contig_1", "start": 770522, "stop": 771049, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCAGAAGATGTTATTGATGAAAGATATAGAAAAGCAATAGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCCAATTTAAATGAAGTAATTGCCCATTTAATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTTTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGA", "aa": "MDIQSEFILNISKKTFEEKNIPEDVIDERYRKAIEKEVINFITNQLLQYQLKTKSSGKEIEMLLNKDVEIANLNEVIAHLMRGKALSSFVGLKLVSKNANLHNYKIINFKNLGEGGKFNLRNLSYILMDNKNVIQETKPSQIRKKVIEIVVNAASKLGLEINNDYQIFAINNDAY", "aa_hexdigest": "16cb6b67b60f78684bdb9d0db65ba5e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20119.024600000004, "isoelectric_point": 8.923278617858887 }, "id": "DFOKDJOJIG_1364", "locus": "GCA013367855_01363" }, { "type": "cds", "sequence": "contig_1", "start": 771050, "stop": 771403, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358", "UniRef:UniRef50_UPI0027330C60", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef90_UPI001314B421" ], "nt": "TTGGAATTAGTTTCAAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAAGCCCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTTGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATACAAAAAAATTCTTTTATTACCAACAAACAAAACAATCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATTGACCCTGATTGA", "aa": "MELVSKYDDPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPD", "aa_hexdigest": "c9f7e66eb051396304f5653fddd5b1cc", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001314B421", "query_cov": 0.8632478632478633, "subject_cov": 0.9805825242718447, "identity": 0.95, "score": 178.0, "evalue": 3e-56, "valid": true, "uniref50_id": "UniRef50_UPI0027330C60" }, "pscc": { "uniref50_id": "UniRef50_UPI0027330C60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027330C60" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 771074, "stop": 771685, "observations": { "mutation": [ 771401, 771494, 771497, 771584 ], "stop": [ 771401, 771494, 771497, 771584 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005C90358", "query_cov": 0.9316239316239316, "subject_cov": 0.4448979591836735, "identity": 0.991, "score": 208.0, "evalue": 3.74e-66, "gene_start": 9, "gene_end": 117, "reference_start": 1, "reference_end": 109, "reference_sequence": "NPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPDWKPNPNPDDEDIVSPEDIPKDPNRIKKVVKTWWFPLVVIVGILTFLVASIFIIKSLLRKYGWSFGKRAKRIRRQNPNKQSYQEKRSNKRQEKLAKKEAKNLKRQTKKQEKANFIQAKKDKKTKNKSKTNNEQNSIN" }, "paralog": false, "identity": 0.9605911330049262, "score": 382.0, "evalue": 3.61e-137, "description": "Internal stop codon at 771401, 771494, 771497, 771584. Nonsense mutation around 771401, 771494, 771497, 771584.", "psc": { "uniref90_id": "UniRef90_UPI0005C90358", "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef50_UPI0005C90358" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13938.78549999998, "isoelectric_point": 5.6632894515991214 }, "id": "DFOKDJOJIG_1365", "locus": "GCA013367855_01364" }, { "type": "cds", "sequence": "contig_1", "start": 772676, "stop": 773296, "strand": "+", "frame": 2, "gene": null, "product": "Mbov_0400 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0", "UniRef:UniRef90_UPI00069702F0" ], "nt": "ATGAATCATCAAAGTGTAGTAGTCACCAAAGATATTTTTATAATTGATAAAGATGTTTTAGAAGCCAATGTAGATCAAAACATCTATCAAGAAACCTTGACATTAAATAAAAACGATAAATCTTTAATAATTAGCGATTTAGAACAAAGGATTAATTCAATTCCACCTGATTTAAATATTTTAAAAATATCTAATAACTTAAAAAGCAATTTAGTTTTATACACTAATGATAGCCTAGTTAATTCTCAGTCTAATTCGATTTTGAAAGGCAAAAATTACAAAATTAAAAAGGCTACTAAAAATTATTACAAACATAATTTTAACAAATCTCAATTTCTTAAAGATACAAACACGTCAAATATAGAACATACTAAGCAAGCCCTAAAAAACATAAAGTTATGCATAAATAAAGCTAAAAAAATTGATTATACTAATTTTGAATATATTTTAGATGTTCAAACAAACACATATCTATTTCATCTAATTCCCTCTTCTCTATCCGATTTTGATAAAACACCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACCAATCTCAGAAAGAGTCCAAAGCTAGCCAATTCCGCTCAAAACCTCATAATCTAAAACATTAG", "aa": "MNHQSVVVTKDIFIIDKDVLEANVDQNIYQETLTLNKNDKSLIISDLEQRINSIPPDLNILKISNNLKSNLVLYTNDSLVNSQSNSILKGKNYKIKKATKNYYKHNFNKSQFLKDTNTSNIEHTKQALKNIKLCINKAKKIDYTNFEYILDVQTNTYLFHLIPSSLSDFDKTPTDLQDSKVINEYNQSQKESKASQFRSKPHNLKH", "aa_hexdigest": "3769969c99795e10e2a3819124aca02d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 772454, "stop": 773296, "observations": { "mutation": [ 772469, 772673 ], "stop": [ 772469, 772673 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00069702F0", "query_cov": 1.0, "subject_cov": 0.7357142857142858, "identity": 1.0, "score": 389.0, "evalue": 1.44e-135, "gene_start": 1, "gene_end": 206, "reference_start": 75, "reference_end": 280, "reference_sequence": "MSLELWKPLKITNPLLEDIFHLNIDTKFKIHPLIIFENKENKTYYCIRLQTSTKTTKKHNLQIDNSSYQTNEYWMNHQSVVVTKDIFIIDKDVLEANVDQNIYQETLTLNKNDKSLIISDLEQRINSIPPDLNILKISNNLKSNLVLYTNDSLVNSQSNSILKGKNYKIKKATKNYYKHNFNKSQFLKDTNTSNIEHTKQALKNIKLCINKAKKIDYTNFEYILDVQTNTYLFHLIPSSLSDFDKTPTDLQDSKVINEYNQSQKESKASQFRSKPHNLKH" }, "paralog": false, "identity": 0.9928571428571429, "score": 553.0, "evalue": 1.03e-202, "description": "Internal stop codon at 772469, 772673. Nonsense mutation around 772469, 772673.", "psc": { "uniref90_id": "UniRef90_UPI00069702F0", "product": "Mbov_0400 family ICE element protein", "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00069702F0", "UniRef:UniRef50_A0A454C8W0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0" ] } }, "id": "DFOKDJOJIG_1366", "locus": "GCA013367855_01365" }, { "type": "cds", "sequence": "contig_1", "start": 773333, "stop": 773473, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATAGATTTTTATATTAATGGTGTAAGCGAAAAAAGTATCTTATCCTCAGGCCAATTAATAGTAGAAAAATTGAACATCGAAGAAGAAATATTTCGTACTAAAACAAGGTTTGAAAATAATCAATATAGTAATTGA", "aa": "MEIDFYINGVSEKSILSSGQLIVEKLNIEEEIFRTKTRFENNQYSN", "aa_hexdigest": "da1f63fc5604fbd82d1459e9fb83b08a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5428.0015, "isoelectric_point": 4.644962120056152 }, "id": "DFOKDJOJIG_1367", "locus": "GCA013367855_01366" }, { "type": "cds", "sequence": "contig_1", "start": 773849, "stop": 774211, "strand": "+", "frame": 2, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005A7E644", "UniRef:UniRef50_UPI0013142BC0", "UniRef:UniRef90_UPI0005A7E644", "UniRef:UniRef90_UPI0013142BC0" ], "nt": "ATGGTTAGAATGGTTGGATTTCACTCTGGATATTTAAACCAAAATAACCATATGTTATTAAATAAAACCATTATTTTTCAGTTTAACAAAAGTCAAAAAATTTATCAAAATATAGATATCTTAGCCACTAAAACCAGCGAAATTTTAAATAACTTTTACCATGCAAAAACTATTAATTTATCAGGAGATGGAGCAAGAATAATAACTAAATTTAGCGAACAAATTAATGCTATTAGGTATTATGATAAGTTCCATTTTCACAAAATATTATTTGATGTTTTTGGGTATTCAAGAAAGAAAAATAAGGATAATAAGAGAATATTTGATAATACATTTGGGAATCAATTTTCTCAATTTTTGTGA", "aa": "MVRMVGFHSGYLNQNNHMLLNKTIIFQFNKSQKIYQNIDILATKTSEILNNFYHAKTINLSGDGARIITKFSEQINAIRYYDKFHFHKILFDVFGYSRKKNKDNKRIFDNTFGNQFSQFL", "aa_hexdigest": "cf62241933c3fbb644ce3d03a7aa80fc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0005A7E644", "query_cov": 0.975, "subject_cov": 1.0, "identity": 0.94, "score": 226.0, "evalue": 6.52e-75, "valid": true, "uniref50_id": "UniRef50_UPI0005A7E644" }, "pscc": { "uniref50_id": "UniRef50_UPI0005A7E644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005A7E644" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 773759, "stop": 774514, "observations": { "mutation": [ 774209, 774332 ], "stop": [ 774209, 774332 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013142BC0", "query_cov": 1.0, "subject_cov": 0.47244094488188976, "identity": 0.992, "score": 241.0, "evalue": 4.85e-79, "gene_start": 1, "gene_end": 120, "reference_start": 31, "reference_end": 150, "reference_sequence": "MKNTKFLPSKNDVIQLENDDAYINLNKGKFMVRMVGFHSGYLNQNNHMLLNKTIILQFNKSQKIYQNIDILATKTSEILNNFYHAKTINLSGDGARIITKFSEQINAIRYYDKFHFHKILFDVFGYSRKKNKDNKRIFDNTFGNQFSQFLWFLNNKKYEDFENKLHENLIYLRENKLSAIKIVEIKSLIKLWKNNKNYLINSYENANYYGGNAETFIGHYLKKYTKTAFSRHNIDYLKLKILLNIPQNLNVVFL" }, "paralog": false, "identity": 0.9840637450199203, "score": 499.0, "evalue": 4.17e-183, "description": "Internal stop codon at 774209, 774332. Nonsense mutation around 774209, 774332.", "psc": { "uniref90_id": "UniRef90_UPI0013142BC0", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_UPI0013142BC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013142BC0", "UniRef:UniRef50_UPI0013142BC0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013142BC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013142BC0" ], "product": "Mbov_0401 family ICE element transposase-like protein" } }, "id": "DFOKDJOJIG_1368", "locus": "GCA013367855_01367" }, { "type": "cds", "sequence": "contig_1", "start": 775396, "stop": 775515, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGAATAGATCACCATCCAGTTGATCCAGATATTAATTATGACTATACTTGGGAAGATGCAACATATGCAGCAGCTGCTGAACAAGTTGGATACATTGCAATGATGGCAAAATGA", "aa": "MLRIDHHPVDPDINYDYTWEDATYAAAAEQVGYIAMMAK", "aa_hexdigest": "a8aae5d778d2622d7d44e34280a6413b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4486.9666, "isoelectric_point": 4.3189905166625975 }, "id": "DFOKDJOJIG_1369", "locus": "GCA013367855_01368" }, { "type": "cds", "sequence": "contig_1", "start": 776042, "stop": 776431, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGAATAAAATAAGTGCAGAAAAATTAGCAATTTTCAAGAAAATTGAAGAAAGAATTAAAGCACACAAAAATATAGTTATATATCATCATATTAGACCTGACGGAGATTGCTTGGGTTCTCAATTTGGAATGAAGAATTTAATTAAGGTAAATTTCCCTGATAAAACAGTGTATACAATCGGTGATTCCAAAGGTATTTATTCATTTTTAGAATTTAAAATGGATAAATTACCACTAGAAAAGTTAGATGATTCATTAGCAATTATTGTTGATGCTAACTTTAAAGAACGTTTAGAATGTAGAGAATACTTGGATAATAATTGTTTTGATGAAGTAATAAGAATCGACCACCATCCAAATGATGATGATCTAGATGCTTCATTAAGATGA", "aa": "MNKISAEKLAIFKKIEERIKAHKNIVIYHHIRPDGDCLGSQFGMKNLIKVNFPDKTVYTIGDSKGIYSFLEFKMDKLPLEKLDDSLAIIVDANFKERLECREYLDNNCFDEVIRIDHHPNDDDLDASLR", "aa_hexdigest": "0c2a38fe4e5df54da82c9499dd708430", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 100, "aa_cov": 0.751937984496124, "hmm_cov": 0.76, "evalue": 1.6381972114345963e-12, "score": 43.433292388916016, "start": 24, "stop": 120 } ], "seq_stats": { "molecular_weight": 15013.060199999985, "isoelectric_point": 5.709670066833497 }, "id": "DFOKDJOJIG_1370", "locus": "GCA013367855_01369" }, { "type": "cds", "sequence": "contig_1", "start": 776660, "stop": 776827, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATATTAATATAAATACATATATATCTCAAAACATGAAAATTGCAAACGACGTCGTTTCATTTTACTTCCCATTGTCTGAACAAATTAAATTAGGAATTAAAGATCCATTAAAAGCAAATAGACCTTTTGTTCTAGGCTCAATTGACAATACAAGGGCATGA", "aa": "MFDININTYISQNMKIANDVVSFYFPLSEQIKLGIKDPLKANRPFVLGSIDNTRA", "aa_hexdigest": "54c5c29184ba42abae513926e9dce02a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6262.1726, "isoelectric_point": 8.0980188369751 }, "id": "DFOKDJOJIG_1371", "locus": "GCA013367855_01370" }, { "type": "cds", "sequence": "contig_1", "start": 777438, "stop": 777980, "strand": "-", "frame": 2, "gene": null, "product": "Phage abortive infection protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X7R6", "UniRef:UniRef90_D1J8X0" ], "nt": "ATGGCGTCAAAAGAAAAGAAAATGAAAATAAAAAATGTTATATTAAACATTATTGGTTTTCTAACCGTTGCTCTCTTTCTCGTTATTGCTATCTTGCTATTTTTAGCAGCAAATGGCATTATGGGAACTATATCTAAAAAATCAAGTATTGTTTGTTATGTTTTTGGCGCAATCTTCTTAGCTATATTTATTTTAATTGTAATTAAAATGATATTAATATTAAAAAAAGAAAATGTATATATAAAAAATGCAATTGATACAGATAAGCTCTTTGCAAATGCTTCTCTAAGCCCTGAAGAAAATGAAATTCATAAGCAATTTATTGAAAAATTTAAACAATATCAACAAAGTAGAAACATATATTTTGGATATTTATTTACTAAAGCATTAAGCTCATATAAAAGAGATAATATTGATATCTCAAACCACGAAATAAATTCATTAATTGAAAAAATGATTATTGACTGCCATAATGAATTTGGAATCTTTGATGTCTATTTGGCAATTGATTTAGCAAATTCATTAAATAAAAAGCTTGTTTGA", "aa": "MASKEKKMKIKNVILNIIGFLTVALFLVIAILLFLAANGIMGTISKKSSIVCYVFGAIFLAIFILIVIKMILILKKENVYIKNAIDTDKLFANASLSPEENEIHKQFIEKFKQYQQSRNIYFGYLFTKALSSYKRDNIDISNHEINSLIEKMIIDCHNEFGIFDVYLAIDLANSLNKKLV", "aa_hexdigest": "07223f783df464662a328a178ef51eb3", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J8X0", "query_cov": 1.0, "subject_cov": 0.8450704225352113, "identity": 0.978, "score": 327.0, "evalue": 3.22e-112, "valid": true, "uniref50_id": "UniRef50_A0A501X7R6" }, "pscc": { "uniref50_id": "UniRef50_A0A501X7R6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X7R6" ], "product": "Phage abortive infection protein" }, "genes": [], "id": "DFOKDJOJIG_1372", "locus": "GCA013367855_01371" }, { "type": "cds", "sequence": "contig_1", "start": 778089, "stop": 778619, "strand": "-", "frame": 2, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:6.3.4.21", "SO:0001217", "UniRef:UniRef50_D5E5M9", "UniRef:UniRef90_D1J8X1" ], "nt": "ATGGCAGATAGGGCAGATCATTATATAAATCAACCAAACGACTCATATGCAATAAGAATAGGTGGAATAGAAAATCACTCGACTTTTCTAAGTTCAAATTTTGATCCAAAACATACTTTTGGAAGCATGCCTCATGCATTAATTCAAATGTTTGACGGTGATATTGTAGCTGCTTGTAAAGCATATAAAAAAGTTTTTCCAAATGATGATCTAGTTGCCTTAGTTGATTTTCACAACGATGTTATAAGCGATTCATTAAAATGTTTAAAGGCTTTTGGCAAAGATTTAAAAAGTGTAAGAATAGACACATCGAAAGCTCTAATTGATAAAATGTTCAAAGAAGGTGAAGCGGAATTTGGCGTTACACCAAATCAAGTAAAAAGATTAAGAGAAGCATTAGACAATAATAACGGCAAGCATGTAAAAATAACTGTTTCAAGTGGATTTGACGCTCAAAGAATTGCTGAATTTGAAGCAGAAGACACTCCTGTTGACACATACGGCATTGGTGCAGCGTTGCTAAAAATTTGA", "aa": "MADRADHYINQPNDSYAIRIGGIENHSTFLSSNFDPKHTFGSMPHALIQMFDGDIVAACKAYKKVFPNDDLVALVDFHNDVISDSLKCLKAFGKDLKSVRIDTSKALIDKMFKEGEAEFGVTPNQVKRLREALDNNNGKHVKITVSSGFDAQRIAEFEAEDTPVDTYGIGAALLKI", "aa_hexdigest": "d03b69b1c6de456cb709ad5cb9d801f2", "start_type": "ATG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "both", "pseudogene": { "start": 777981, "stop": 778919, "observations": { "mutation": [ 778091, 778658, 778718 ], "stop": [ 778091, 778658, 778718 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8X1", "query_cov": 1.0, "subject_cov": 0.5238095238095238, "identity": 0.9890000000000001, "score": 352.0, "evalue": 2.41e-120, "gene_start": 1, "gene_end": 176, "reference_start": 125, "reference_end": 300, "reference_sequence": "MKKINLKDYISIYFYKTQKISKKFKKDQNITLQFFQRHNDVKLCGINEAIDILKQNTDISKYTIRYMPEGSIIHNREVVLELEGPYYEFGIWEGIIDGILARQSSIATNAYWAIKAANGKKIVSMADRADHYINQPNDSYAIRIGGIENHSTFLSSNFDPKHTFGSMPHALIQMFDGDIVAACKAYKNVFPNDDLVALVDFHNDVISDSLKCLKAFGKDLKSVRIDTSKALIDKMFKEGEAEFGVTPNQVKRLREALDNNNGKHVKITVSSGFDAQRIAEFEAEETPVDTYGIGAALLKIWVNFSADATRLNGHNIAKAGRGYLKNPQLITYKEAK" }, "paralog": false, "identity": 0.9807692307692307, "score": 608.0, "evalue": 8.58e-224, "description": "Internal stop codon at 778091, 778658, 778718. Nonsense mutation around 778091, 778658, 778718.", "psc": { "uniref90_id": "UniRef90_D1J8X1", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "6.3.4.21" ], "uniref50_id": "UniRef50_D5E5M9", "cog_id": "COG1488", "cog_category": "H", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8X1", "EC:6.3.4.21", "UniRef:UniRef50_D5E5M9", "COG:COG1488", "COG:H" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5M9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5M9" ], "product": "nicotinate phosphoribosyltransferase" } }, "id": "DFOKDJOJIG_1373", "locus": "GCA013367855_01372" }, { "type": "cds", "sequence": "contig_1", "start": 778716, "stop": 778991, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02749.22" ], "nt": "ATGAAAAAAATCAATTTGAAAGATTACATTTCAATTTATTTTTACAAAACTCAAAAAATATCAAAGAAGTTTAAAAAAGATCAAAACATTACTTTGCAATTCTTTCAAAGACATAATGATGTCAAATTATGTGGAATAAATGAAGCAATTGATATTTTAAAACAAAACACAGATATTTCTAAATACACCATAAGATACATGCCAGAAGGCAGTATAATCCACAATAGAGAAGTAGTTTTAGAACTTGAAGGCCCTTATTATGAATTTGGTATATGA", "aa": "MKKINLKDYISIYFYKTQKISKKFKKDQNITLQFFQRHNDVKLCGINEAIDILKQNTDISKYTIRYMPEGSIIHNREVVLELEGPYYEFGI", "aa_hexdigest": "79ced7de4ca5cefb382cfa3e22346b6c", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02749.22", "name": "QRPTase_N", "length": 79, "aa_cov": 0.8681318681318682, "hmm_cov": 0.8481012658227848, "evalue": 4.2906629856055145e-11, "score": 38.59199142456055, "start": 12, "stop": 90 } ], "seq_stats": { "molecular_weight": 10911.543199999996, "isoelectric_point": 9.16245632171631 }, "id": "DFOKDJOJIG_1374", "locus": "GCA013367855_01373" }, { "type": "cds", "sequence": "contig_1", "start": 779316, "stop": 779573, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8X2", "UniRef:UniRef90_D1J8X2" ], "nt": "ATGGTAAAATATCCATTATCTATAATATTTATAATTCTTTGCATAGCAATGATAGCAGGAATGTATACAATAAATGTAATGGGTTATAAACTAGCAATCAAATTAAACAATGAATCTTTAAGAAAATGCTATTTGATTTCACTAATTCCAATATTGTTTTTTGTAGCATTAGTAGCAAGCTTTGTGTCTTCAATTAAGCTAAAAGAAATTTATATAGATAAAAAAAGCCAATTTCAAAACGTTGCTATAGCAGGATAG", "aa": "MVKYPLSIIFIILCIAMIAGMYTINVMGYKLAIKLNNESLRKCYLISLIPILFFVALVASFVSSIKLKEIYIDKKSQFQNVAIAG", "aa_hexdigest": "bda6291628b543d72b436c13c4524621", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 779148, "stop": 779573, "observations": { "mutation": [ 779229, 779244, 779286 ], "stop": [ 779229, 779244, 779286 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8X2", "query_cov": 1.0, "subject_cov": 0.6028368794326241, "identity": 0.988, "score": 155.0, "evalue": 5.29e-47, "gene_start": 1, "gene_end": 85, "reference_start": 57, "reference_end": 141, "reference_sequence": "MAKNQDKKMLSPEEKEIENIKKISKNGWHFSYWSLFFILGVIAILIWAVESESSSSMVKYPLSIIFIILCIAMIAGMYTINVMGYKLAIKLNNESLRKCYLISLIPLLFFVALVASFVSSIKLKEIYIDKKSQFQNVAIAG" }, "paralog": false, "identity": 0.9645390070921985, "score": 256.0, "evalue": 3.05e-89, "description": "Internal stop codon at 779229, 779244, 779286. Nonsense mutation around 779229, 779244, 779286.", "psc": { "uniref90_id": "UniRef90_D1J8X2", "uniref50_id": "UniRef50_D1J8X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8X2", "UniRef:UniRef50_D1J8X2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8X2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9565.66899999999, "isoelectric_point": 9.555197715759277 }, "id": "DFOKDJOJIG_1375", "locus": "GCA013367855_01374" }, { "type": "cds", "sequence": "contig_1", "start": 779744, "stop": 779944, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTACCTTACCTATAATACTTGTAATTGTTGGTTATATTTTAATTACATACATATTAACAACATCAATACTGTTAATTAAAAAACCACAATTAATAACTAAGCGCCACACTTTAATTTCACAAGTTGCAATAGCGTTTATTCCAACATTAAGTGTTTATGCTATAACTTCATTAGATAATTATTTTATCCTATGA", "aa": "MKITLPIILVIVGYILITYILTTSILLIKKPQLITKRHTLISQVAIAFIPTLSVYAITSLDNYFIL", "aa_hexdigest": "e2e1637c80384065b21d77ff249fd417", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7445.1069, "isoelectric_point": 9.777226829528807 }, "id": "DFOKDJOJIG_1376", "locus": "GCA013367855_01375" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Metamycoplasma hominis] [strain=FBG] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TTAAGCTGCTTTTGCTGTAAGCTTTGGTTCAACTTTTTGTTTAAAGAATTTTGTTCTTTGGAAATAGTGAACAAATATATGTTGCAATATTAATATAAATGCTCCAACAATTCAATAAATTTGTAATCCTGCACTAAAGAATGCTCCAAAGATTATAAATACTAATGAAATAATATTTGACATACGGTTTTGTTTTTTCATTGCTGCTTTTTGATAAGCATCAACACGTAATGATTTCTTTTTCTTTCAATTTAAAATCTTTGGCATATATTGCGATAGTGCTTGCATACCGACAGATATTAAAATTATTGGTAGATATATGAATTCTTTGTTAGAAAATACCCGGTTTAGTGAATTTGCTGATAATTGAATTGAATATCAGGTTGCTTGTTTTATTTCAGGAGAGGTATTTATAACTCTAAATACAACAATTAATATTGGCAATGCAATAAACTGAGGCCAAATCTGTGATCAAGGAGAAATTTTTTCTTTCTTATATAATTCACTAATTTCAGCTTGCTTACGTTGCATCATTTGTCTGTCGCCTTTGTAAGCAGCATATTTAGATTCAATTTTGGCTTTCTTTTGGTTGAATTCTTCCATTTTTGCTTGACTAAACAAAGACTTAAATGAAACACCAAATACTACTAAACGAACAATTATAATAACAATTATTAATGCTAATATAATACTTCATCCTGTTGTTCCAAGTCCTTTAATAATTGAGTCCATAAATAAGTATATAGGTTGAACAAATATACCATAAAATGGTCCTTGTGCTCAATATTCTTTGTATGTTGAAATAGGTTTTTGACTCAATATTGCGCTATTATTAAATATTGTGTTTGTTCAATATTCTGAATTGTAATTTGAAGCAGGACTTAGACTTGATTTTCTATATACAAAACTACCGTTATTTGTTTTAGAAGGTAATTTTACATAATTTACATTTGATAAGTATTGTTGGAAAACACTTTTAACAAAAGTAAATAGTTGAAGCAACATTCTTCCTTCATCTTCATTGGCTAAAAGAGTTTTATTTTTTGCTTTTTGTTCATAGCTTCTTATAAATTGATTTCATTTTTCAATTATTTGGTTAGCATTTTCACTACCATTTAATTTTAATGCTTCTTTAAAGATGTTAACCTTATCATCCTTAAAGGTTTTCAAACTTGCTCATTTTTGTAATGTATTTGAAACAAGAATTTGAAAAATATCTCTTAGAACAATTTCATCAACTGATGAACCACCAGTTAACTTTCCTTCTACTATATCATAGTTAAATTCTTTTATTTTTGTTCAATCTGTTGGTATAATGCCATCTTTATCTTTTGGCTTAGTGTCAAACGATAAAGAATTAGGAATTAAAATTTCACTGAATTTGGTTAATGGAGTGTAATTTTTGGTTTTGTAATTTCCCTTTTCATCACCCAAATTAAAGTAGGCATAATTTCCATTTACATTGTAGATGTAGCCACTTTTATCAGATGTTCCTTTTTCTGTTATATTTGTTCAATCTTCATCATGAGAAGATAAAACTTTTGCTTTGTCGTTTTTTGGCATTTGCAATTGCAATCCAAATGAACGAGAACCATATGCGCCATAATTAGGATCTCTCTTCCTTAACTCATTAATAGCATTAGGTTCTTTTAAAGCAAGATATGTATTGGCGATAATATTTTTATCATTTATTTTGTTTGGTAAGGTAAAGAAATTACCAGTGCTATCGTATCTATAAGTGACAATATTTGGTGAGATGTCACTCTTTTTAGAATATAGCTCTTGTCCAGAACCGACTTTATTACTTGATTTTACAGTAAATGATTGTACACAACCAACTAAACCGATAGCCAAAAATAATATAACTATGACAATTTTTATTCATTTTCAAACTTTTAACCAAATATTTTTCGGTGTTTTGTTTTCTGGTTGACGAACTCGAAAGTCTTTATAGAATTTAGAGACATCCTTATTTGCCATCAGCGATCCTTTCATAAAGTTTTTTTACTTCTTTAACTTTTTGATTCCAGTCAAGACTCAAGAAGCCTTTGCGAGCAATAATCACAGTTTCGTAATTTAAAGTGCTAAAATCAATGTTTCTTAGAATTGATCTGATTTGATTTTTGTAATGATTTCTCAAAACAGCATTTGCAAATTGCTTAGGGATTGAAATACCCACTTTAAAGAAATCGGATTTGTTAAAATAAATTATTAAATTTCTTGAAATCACTTGATTGTGAGAATCAAGAATTTTTTGAAATTCCCAATTTTTTTTAACAACGTTATTTTTATTTATCATAATTTAAAAATATGCAAGGTATTAGACTTATTTATTGTCTGATACAGTTAATGTATATCTACCTTTTGCACGTCTAGCTGCTAAAACTTTTCTACCATTTTTAGTTTGCATTCTAGCCATAAAACCATGAACTTTAATGTGTTTGCGTTTTTTTGGTTGGTATGTTCTTTTCATAACAATTCTCCTTTGCGTTTTTATATATAAAATAACTTAATTTATTTTACCAAAAATACGACAAAAAAAGTATATAAAATACCTAAGAAAAAAAATTTAATATTTTTTATATTTTTAATGTAAATTAAAAGAAACATAAAATTTAAAGTTAATAACAAAATAAACAAAGAAATTATTTAAATGTTTATTATGTTATTTTAGTATAAAAAACCTCTTATTTTAAAAACAAAAGCCCTAAAAAAAATGTTAATAATTTTGTTAATAATTTGTAAATTAGCATTTATAAGGGCTTATTAATTTATTAATTTTTAATTTTAAAATATAATAGAGGTATGAAAACTACAAATCCCTCTGAATTAGATTTAAGAACACATAATATGTCATTTCAAAAAGAATTAGTTAATACTGCAAGTGATCCTTTAATATATGATCAATTCTTGTCGTCATTAGAAATTGTAAAAGAAAATAATAAAATGATTTACATATTAGTTAAAGGACCTGAAGACGTTTCAACATATATTAAGAACAGTCACAATGAAAGTATTAAAAGAGCAATTAAAAATGTTTATGGTGAAGAATATAATTTAGTTTATATTAGAAATTTAGATGAAATTAAAAGCTTGCAAGATAGCAATAATGTTGAATCTGCAACTAATTCATTGGCTAATAATTCTAAAGAAAATTTTAGCAATATTAAGAAGAATTTAAGATTTGATGATTATGCAATAGGAAAATTTAATAATATGGCTTTAAAGGCAGCAAAGGCTATTTGCAATAGTGAAAAGATATTGTTCTCTCCTTTATTCATTCATGCTTCAAGTGGGCTTGGTAAAACACATCTATTACATGCTATTGGAAATGAATTATTAAAACACGGTAGAAGTGCATTATATATAAATCCTGATTCATTGACAAGAAGATTAGTTGAACAATTAAAATCAAAGAATCAAGAACAAATTAATAAAATTATTGATGAATTAATGTCTTATGATTGCCTAATGTTTGATGATGTTCAACAATATGGTAATCGTGATTCTACATTAAATGTCTTATTTAATATTATTGATAATATGATAATGAACGATAAGCAAATAATCTTTTGTGGTGATAAAAAACCAGATGATTTAGGTGGATTTGAACAAAGATTTATAACTAGATTTAATGGCGGATTAACTGTTGAAATTTTAAAACCAGAGTTAAATGATGTAATTAATATCTTGAAATTTAAATTGCAAGAAAACGGTATAAATCCGGAACTTTGAGAGGAGGAAAGCTTAAAATTTATTGCTAGAAACTTCTCTTCATCAATAAGAAATATTGAGGGTGCAATTAACAGAATAAAATTATTTCAAGATGGTGACGACGGCTTTTTTACATATGATATTAATACAATAAAATCAATATTTAACAGCATGACTCAAGTTGCTGAGAATATTACCCCCGATAAAATAATTGATGCTGTTTCTAAATACTATAAAATTGACAGAAAAAAAATATTGAGTAATGTAAGAACAAATGACGTGGTTAATGCCCGCAGAATAGCAACATATCTAGTGAATAAAAATTTTAATTATACATTGCAGGAAATAGGCAAGGTTGTAGGAAATCAATCACATTCAAACGTTATTGTTTCGCTTAATTGAATTGAAAAAAATGCTAACAGTAATCCAACGCTTAAATTAGCATTGCAAAAAATAGAAAGCAATCTTAAAAAAATTTCGTAATAGGTAGGTATAGAAATGAAATTAAAAATTGATAAATTGTTATTGGATAATGCAATTGAAAGAGTATCAAAATCTGTGGATGCTAATCCATTTTTACCAGTCATGAAAGGTATTTTAATTCAAGCAGAAGATAACAAAATAACATTAATTGGTTCAAATGGTGAAACAAGCATAAAGCATGAAATAGAAACATCGATGGATGCCCAAATTGAATTGCCCGGAATTGTATTAGTCGAACTTAGCTTGTTTAAAAATATTATTAAGAAGTTAGATGGCGAAATAGTATTAGAAGTTGTAAATAATATTCTTAAAATAAGTACTAACAACGAAAATTATAATTTAAATATATATGATGTAAATGAATATATTGCAATTGATTTTGCATGTTATGGGGAACAAGTAAAAATTAACTGAAATGATTTTAAATCTTTAGTTCGTGATGTTATTTTTGCCGCTTCAACAAACGAAGTTAGTCTAATCTTGTGCTGTGTTAATATATCAGCAGCCAATAAAAAAATAAAGTTTATAACAACAGACAGATTTAGATATGCTCAAGCAGTTCAAGACATTTCTGAAGATGTAACATTCAATATTTCAATATTAGCTAAAAACTTAAGAGATATTACCAATTTTGATTACAATGGCGATGTTATTTTAAATATTTCTGATCAAAAGATTTCATTTAAATTAGACTCAACAACTATTGAATCAAAAGTTGTTGATCAATTGTATCAAGATGTTTCAAGAATTGTTCCACAAGAATTTGACACTGAACTTAAAATAACCAAAAAAGAACTAAATAACTTATTGAATAAGGCATCAGTTATTATTAATGAAAACTATAATAAAATTAAGTTGCACATTGTTGATAATGTTTTAACCATTTCATCTGCTAGAGAAGAAATTGCTAATGCTGAAATTAAAACAAATAATTTTTCTTACAACGGTGAAGAATTGAAACTTGCATTAAATAGTAGATTTTTAAAGGATGCTATTTCTGTATATGAAGATAATTTGAATATTTTATTCACAAAAGATAAATTAAGAATAGTCATAAAATCAGATTCTAACCCAAATGTGCTACAATTGATTACGCCACAAAAAGGATTCTAATGGAAATAGAAATTACTGGAGAATTTATTAAATTAAGTCAATTTTTAAAGAAAATTGATGTGTGTCCAACGGGCGGAATGGCAAAATATTTTGTTCAGGTTCATAGAATATTAATTAACGGGAACCTTGCAAACGGAAGAAATGCTAAGATTTATCCCGGCGATACCGTTTGAATAGATGACAATGTTTATTTAATAAAAGGACAAAAATAAAAATATTTTTTATAAATATTATAAAATATTATAATTATTAAGCTTAACAATAACATAAAGGAGAAATCATGCCAGGAAGAGATCAATTAACAGGTCAAAAAGCTCTAAGTGGAAACAAAAGATCACACGCTTTAAACACCGCAAAAAGAACATTTGACTTAAATCTTCAAAAAGTTACTGTTTTAACTGAAACCGGAAGTAAAAAAACAGTTCGTGTAACTGCAAAAAATGCTAGAACACTAAAAAAATATGGTCTAGTTGCATAGTAGTTTTTTATAAAAATAATATGAAGTTGCTTAGGCAACTTTTTATTTTATTTATGAAAAAAAAGAATAATAAAAAAGCGCAACTCTCTTTCTTAGTCCATACTAAAGAGAAATTGCATAATTATTATAACAAGTTATAACAAATTATTAATAATAAGTTTTTTAATTTCATAATAAATGTAATTTCTCTAAATATTAAGGAGAATTTATTATGAGAAATATAGATCAATTAGAAATCTTTTATGCTGAATTTGAAACGAAGGTTCAAACACATAACAGAAGTGCTAAAACACTTAAAAATTATACATTTGTTTTAAGACAATTAAGAGACTGGGACACCCTCGAAGAATTAATAGAATAAATTAATTTTATTTTTAAAAAAATTAAGCAGTTGGCTTAATTTTTATTGTTTATAAATTTGAATAAATTAATAGAAATTTCTTTTGTAATTTTTTCAGCTCTTATATTTAAATCTAGTTCATTGCTTTTTAAAAATTCAGAAATTTTTTGTTTTAAATTGCTTGGAAAATTATTTAATAAAGTTTTACGCTTGTATTGAAACATTTGTTTAATAAATTTCAAAATCTCAACTTGATTATTTCAAATAAAATCATTATTCTTTATTAGATTTAGTTGAATAACTGCCGAATCTACTTTAGGAGCGGGATTAAATTCTTTTGCCTTAACAGTGAATAAATATTTAGGTTCATAGAGAAGTTGCATTGATGCTGCTAATTTACTATATTGAGAATTGCCTGCTCCACAGCATATTCTATCTGCTACTTCTTTTTGAACCATTAATGTTATTTTATTAATGTTTTTAGCGTTATCTAATAATTTAAAAATTATATCGCTTGTTATATTGTAAGGAATGTTTCCAATTACTTCAAAATCAGTATATTTTAATAAATCTGTTTGTAAAAAATCTTGGTTCAATATTTCAACATTGCTTGAATAATTTTTTTGTAATAAAAATTCGTATAATCTTTTATCAAATTCAATACATACAAGTCTATTTACAACATCTGCCATTTGATCAGTTATTGCGCCCATTCCCGGGCCAATTTCAATAATATTTTTGCCTGCAACGTTCGCAACCTTTACAATCTCTTTTTGAATGTTTTTATTGCTTAAGAAATTTTGGCCTAGTGATTTCAATGCCTTAATCATAGATTTTAAAAACCTTTCTAACATTTTCTTTAATTTGTTCAACTAAAACATCAACATCAATTTGTTTTAAATTAGCAATGTATTCATATGTGTATTGAACAAAAGGGCTAATGTTGGGTTTTCCTCTCATTGGAGTTGGGGCTAAATAAGGTGTATCAGTTTCACAAAATATTCTATCTAAAGGAATATTTTTTACTGCTTCTTGTAAAGGCTTAGCATTTTTAAAAGTAACAACCCCGCTTATTGAATAATAGATATTTGAATATGTCAAGCATTTATTTAAAAAACTAAGATCCCCACTAAATGAGTGAAATATAAAATTAATGTCTTTATATTGAGGCAAGGTTATTATATCTAAAGAATCATCTAAAGCATCTCTAATGTGCATAATTACTGGTAAATTATGTTCTTTTGCAAAATCTATTTGGGCAATAAATCCTTGTTTTTGAATTTCTTTGCTAGGCGAATCATCATAATGATAATCAAGACCAACTTCCCCAATTGCAACAACATTTGGTTCATATATCTTGTTTAAAATTTCTCTATCTTGCAAACCGTTTGATAATGTTGGATGTATTCCAATAACAGGATAAACAAAATCAACCTTTTTAGCAAGTTCTATTGCTTGTATCGAATCATCTTCGTTTGTTCCACATACAAAAAGCCTTTCCATATTTAGGTTATGCCATTCATTAACTATTTCAAGAGGATCTGAATAGTATTCGGTTGATGGGTGGGTGTGTAAATCGATAAATTTCATTATAATTCTCCTAATATATCTTTAAAAATATCAATTATTTTTAATTCCCAAATTGCTTTTGATTGCCTATATTCTAGGTTATTATTAGATTCCAAACAATCTGAAATTACCTTGAATGAATAAAATGGTTTGCCAAAAATAAATGATGTATGTGCCAATGAACAACATTCCATATCGCCTAATAAATTATTTGTATTTCAATCTAATATATTTGAATTTATTATGCTTTTATTAATAAAACTGTCACCGGTTATAATATTGGCTTTTTTAAAATTGCTGCAAATTTTTAGATCTTGATTGCAATGAAATACATCTTGCAAACTAGGCAATTGCCCTATTTTATAATTAGGCAAGGCGGTTAAATTAACATCATAATATTTGCAGTTTTGTATGCAATAAATATCTCCAACACAACCATTAAAAATTGATATAGCTGGTCCAATGTTAATTACTTTTAATATATTGAACTTAGTCAATAAATAACTTAACATTAAAGCAGAATTAGTTTTTCCAACTCCACAATGTGCTAACAAAATTTGTTTATTGTTTCAATTTAATTCATAAAGATTCTGAAAATTATTTTCAAAATCAATTTTTATTTGTTTTAAATGCTTGCTATATTTTATTCTTAATTGTTCGGTTTCTAGTGGTTCGGCAAAAATAAATAAATAAAATTTCTTTTCCATATATTTTTATTATTATATGCATAAATAAGTAAATAACTTACATTTTTATTGTTAAAACGCAATTAGAAAATAAATTTTGCTTTTAGATAAAGTTTTTAATTGCAAAAAGAATTTATTTTAAAATTAAAATATATGAAAATATTGTTAATCATTATTTTATGTGTTTTAATTATTCCAATTTTTATTGGCGTTCCTTGTATATTTTTTATTAAAAAACGCACAATTTTAAAAGAAAAAAACTTCAAAAAAGAATTAGGCGATAAGGGAGAACAAGAAGTAATAAAAATATTGCAAAAAATGAATGCAAAAAAATACCTAGTTTTAAACGATATTAGGTTGATGGGAGAAGGGGGCAAACGAAAACAAATAGACCATATTGTAGTATCCTCTAAAGGTATTTTTGTAATAGAAACAAAAAACTGAAAAGGGGATATTGTTGGCTCAAAAAATGAAAAATATTTGGAGGTAGTTGCTAAGTATGGCGATGATAGAGTACGAAAATGATTTAAGAATCCAATATTTCAGAACAAAGCCCATATTGAAGCATTAAATAGTATACTTTCTAAATCAATAAATGAAAAAATAAATTATTATTCAATTATTGTGTTTGTTAATAAGAATAAAATATATTTTAGAAATGATGAGTCGAATGATACGGAAAAAAAGTTATATTTTAAAGAAAAGATATTTTTAATACGTTTAGATGAAGTTTTAAGCACAATTTATCGTTGCATTAATAATAAAGTTTACAAAAAAACAGCAAAAAGTATAAATATTAAGTTAATAGAAAATATTATTCATAAAGCAGAAACTAAAGAAAAATAAAAAGCGTAATCAAATATAATCCACCTTACCTTTTTTTACAAAAAAATGGAAATATAGATTAGTTTATATTTGCTAAAATTAACAGTGAATTAAAAAAACTATTTTTTTAAAAAAATATTTAAAAATGGCTTATAATAATTACGCTATTAAATAAGAGCCACTGCCCAGGTGGCGGAATGGTAGACGCTACGGACTTAAAATCCGTTGGCTGTAATGGCCGTGCTGGTTCAAGTCCAGTCCTGGGCACCATAAATGCGCCCTTAGCTCAGCAGGTAGAGCAAATGGCTTTTAACCATTGGGTCAGAGGTTCGAATCCTCTAGGGCGTACCATTTCAAGAAATTGATCAAGAGAGCGTTGCTCTCTTTTTTATTATTTTTATTTATAAAAAATCAAGTTTATTTTTAAACCTGATTTTTGAATTATTCAACTATTGATTCTAAATATTGAAGTTTATTGTTTTGACTATCGAATTTTATTTTAGCTCCATAAGCAATAATAGTTTTTGGATAACAATGTCCAAAATTAACATTGTAAATTATTGGAATATCAAAAGGAACAACTTCTTTTCAAATTTCTTTATATTCATTATAATAAACACGATTTTGAGGGCATCCGATTAATATTCCTTGAATATTTTTAAATAAGCCAACTTCTTTGAATTTTAGCAACATCTTTTTAAGTAATTGTGGGCTTGGCCTTTCTTCACTTGTTTCAAGAAATAAAACCTTATCACATAGTTCATTAGGTTTCAATATTAAATTATATTTTTTAAATATTTCAGCTTCTTCTTTGTTTTTTTCTTCATCTAACCCACTATAAATAGTTTCAATGCAACCACCTCATAAATATCCCTCAAAAGATTTCTTTTCATTTAAAAATTCATATTTTGTAATGTTTCTATGTTTCTCTCTTGGTATATTCAATGCTTTTTTTGAAAAATCATTTCGTTCTTCATATCAATATGCACTTGGCTTAATTTCAAATTTAGTTTTATTATCGAATAATCATTTAAATGAGTTTTTAGTATAAGGCAACATTTCTTTGTCAAGTTCAGCAATATCGCATAAAATGTTGTGACCATAAAATGTTTTTAGTCCTAATCTATAAAACATTAAATGGTTAACGGTTGAATTTGAATATCCAATAAAAATTTTAGGATTATTTAAGACTAAATTTTTAAAGTTTTCGTCTTCTAATAAAAGTGGAATAGTTTTGTATGTGTCAAATCCACCAATTGCACAAATTATTGCTTTTACATTGACATTGCTAAAAACTTCATTTAAATCCTCAACTTTTAACATAGGATTTTCTTTTAAAACTTTTATTCCTTTAAGTGCGTTTTTAGCAATCATAACGTTAAGTTGAAAATCCAAATTGAGTCTTTTTATTGCTAAATCTAATTGGTGACTAGCAAATTCTTCTCCAATAGTTCCTGCAGATAAGCTTACTATTGCAATGCCATTTTCTTTACTTAATTTTATTGGTTTGATCATATAGAAATTATATTGAAAAGTTTATTAAATTAAAACAAAAAAATTAGACAAGCAACTTTGCACAAGTCTAATTTTTAGGGCTAGATTTAATTATTTATTGTTTTGAAAATATTTGAATAATGATTCAATATCTGAAATAAATTTATGTTTATTGATAATTAATTCTTTTATGCCGTCAGGAATAGATATATTGACCTCTTTATCAAGTTCTGCATTATCACTTGCCCCTAAAAAATAAAAGTCTTGGTTTTCTTCATCAACATAACAAATTAAAAACGTGTAATTATTATTTTTTTCTACGTCATCAACAAATAAAGCATTATCAGGTTTTTTATTATCTAAATATTTTTTATAGCTAGTAACTATGTTTTTAGTTTTTTGTTGATTATAATCGTGTTTTGCACTTTTAACTTCAATATATAAATAATTTGTTACTTCACCACTATTTATTTTAAAAACAAAATCAGGATATGAAGACTTAATTTCTCTTTCTTCATCAAAATATTCTAATTTTAAACCATGAAAAACAGGGTTTTTTGTATAAACTTTGACGTTTATTTCATTTTTATATTCACTAATTATTTTCTTTGCTTCGTTAATGAATAATGTTTCGGGATTTGAATCAAAATGATGAAGTCTATCGTTTAAATCTTTATATGCATAATTTTCTGTTTTGTTTAGTTTAACATTATTTTTTTCTATTTGATCAGGGTCAATAAATATTTCATTAGTTTCAGGCAATGTTTTATTGGCTAATATTTTATAAAGTTGGTTTGTTGAATCTATTTTTTCAATATATTGAGCATATTGTAATTTAAATGTATTGAAATATTCTTTTATTAGGATATATCAAAAAATATTTCTTTTTATTGGACTATTGTTTGAATAAATAGAATCATATTTTTCATTTAACGCTTCAATAAGAACTTCGTTGAATATTTTTTTATGCATAAGAAGAAATTTGTTGATAAATAATTCTAATTCAATTGAATTATTTATTCATGAATAAACTCTAATTTTATCTTCAATGTTTCCTTGTTCTCCTATTAATTTCTTGTTGTTTTTAATATATTCTTTACTGTATTTTTTAAAATATTCATCAATTTTTTCTTTGACAATAGAATTTTTAGCTTCTCTAATAAAATCTGCTCCTATTATTGCTTGTTTGATTTTTACTTTATTTATTTGGCCATAAACAAATTTTTCATTTTCAAATTCTCGTTTTAAAATCAATGTTCTCCTTATATTTTCTTTGGTTTGAGCATTTGAATAAATATAGTATCTATTGCCTTCTCTAGAAACTTGATCTTTGTTATAACTTGGATTTGGGAATCTTTTAATTCTTCCTATTGTTTGAATAGACAAACTACTTGATGACACTCTTCTAAGTTGCACAAGCATGCAAGCCCTCGGAATATTTCAACCAGTTGAAGGTCCAATCTTAAAAATTATTGCATCAACATTAGAGTCATTTTTTGAAATTTCTTTTAAGTTTATTTTGTGATTTTCTTCCTTTATTAAAGAAGAAGATTTCACTTTATCATTTGAAAAGTATTTAACATAACTCAAATTACATTCATTTAATTTTTTAATTATGTTCTCAATGCTTTCTTTAAAATCATCTTTTTCTTTGGCAGTTGATTTTTCACTTGGTTCATTTTCAACTTGAATAAGCATTGCTGGTCTTATATTTTTTAAATCACTATTATTGTTTGCATATTGGCTTTGAACTTCTTTAAATGTTTCACATGCTTGATTTAGTAATTCATTATCATCAATTAAATCTTGACTTGAGTCAGTTATACCCTTGTTATAAATTAATTCATCTTTTAGCAATTTAATATCATCTTCGGCTAAATCCTTTTCATCAATATAGACAAGTTTATGTGTTCCCTTAGGAGTTGCTGTCATTTTTATTATTAAATTGGCTGCTTTTTGAATTTTATATTCAAATCTAACTTCTTCTTTTTTTAATTTAGATTCATCATGGATTGTATCAATATCTTCAAATTTAAAATTACTTCTACCTTTTATATTTCCACCATGATGTGCTTCATCTCTAATATAAACTAATGTATAGTTTTGATATTTAATTTGATCAAGAAAGCTTTCAAGCAAACCTTGTTCGGTAAATATTCTATTTTTACCAAATGATGAAACACCGAAGATTATTACTTTATTTTTTTGGGGAATTAAATTATAAGTTGAATCTTTATTTTTTTGAGAATTCAAGGAAGGAGATTCCTTTCTTTCTACTTCATACAAACCATTAATATATCTTTTATATTCATTTAGGTTTGATTCCATTTGATAAGGCAAATCTGCTGATGAAAGTGTAGCAATAACAAAGACTAATTTGTTTGGTTCACCATTGGCATTATTTTCTTTTATTATTTCATTTATAGTGTTGGCCAACATAAAGGTTTTACCGCTACCAGTTGGAGCTTGAAAATCAACTATTTTAGGTTCGCTAGATTGAATTGCAATTTTATATTCTTCAAGCAATTGTTTAACTGCTTTTTCTTGTACACTAGTTAGATTCATTGCTATCTTTTCTTTCAACTGGTTTTAAAGCAGTTAAATTTAATAAAATTTCTTGTTCGTTTATATTGTTAATTTTTGAAACTTCAAAATCATTAAGCATTTTTATAAAATCGCTTTTAATTGCTTCGTTGTTATTGGCAAACGCATCAGTATCATAATATTGAATTTTAAACACATCAAGGTTTTGATTATAAGGTTCATTTTTCTTTATTCAATCAAAGTCGGTTTCACCTTTACTTCCACTGCCTTTATTAATTCTATAAAGCCTTTCATAGCAAACATTTATACCAATATTATTTTCATTATTTGTAACTAATGTGAAGGTTCTATTGCCCCCATCTTCTCCATTAAGATCTAAAACTGCATGTCCGGTTGTTCCTGAACCTGCAAAAAAGTCAAGAATGCGAGCATCAGGATTAGGATGAATGTTAATTAAATACTTAATTAGTTCGATTGGTTTGGGATGGTCAAAAACTTTTAAATTAAAAATTTCTTTTTGAAGTGCCGTACCCAAAGAACCATGGAATGAATATAGCAAATTAGAAAATTTTGATGTTCTTTGAACTGGTTTATTATCATTATCTACCAATTGATATTGCTTAGTATAAATTTTATTACCCTTAAAAACAACAAAATTGTTTTCTATTCCTCATTGAACTTTTTCATTGCTTCATCTTCACTGTCAATCTTTGATTGCATGTTGGCCATTTCTTCTTTTTTGTCAATTATCTTTTGAACCACCGGCATAATATGTTATTCCGTTATATTCAATTGGATAGTCTAGTCCCTCAGATCAAGTTAAAGATCCAAAATCAAGTTGTTTAAGTTTATACATACCTCTGGTTTCAAAATATTCATCTCTTAATTTGTATGAACCATCATCAATGTTTTTTGAATAATTATTTGTATTCATATGTTCAATATTTTTTGCATACATTAAAATGCTTTCTGAAAGTATTTTAATGTATTTTGCATCTTCTGCTGAACCTTCGTTTTTCTTTTGTCACACAAAATTACATACAAAATTTTCTTCGCCAAAAATTTCATCCATTAACACCTTTAAATATGCTTGTTCATTGTCGTCAATTGAAACAAAAATAACGCCATCTTCTTTTAAAAGTTGGCGAGCCATTTTTAATCTTTCGTTCATCATATTTAGTCAACCTGTACGACTAAATTTGTCTCTATAAATAAATTTAGATGCCTCAACATTATCTTTTTCAGAAAGATTGTTGCCGTCATCTTTTGCACTGTCGGTATTATAAGGAGGATCAATATATATTAAATCGTAATTATAATCCCCCCCCCCCCTATTTGTTCTTTTTGTCTTTCAATTGCAAGTAGGTTTTTTAATGCATCATAATTTTCACCAATAATTAAAGTGTTTTTATTAGTATTCAATAAATCATTTGTAAATGATAATTTTTCTTGCTTTTTTAATATTGCAATTTGTTTTGTATCAACACTAGGTGCAGCATCAAAAACAAAGCCTGTTTTAACTCTTTGAATCAAAAGTTGGAAAACATTGTCTAAGTTTTCTTTATTTGAATTGTTTGTGTTAACTAAAATTTCTTTTATTAATTTCTTTTGGTCTTGATTTAATAAAAAAGAATTCAAGTTATCTATATCATCTATATAGTTTTTTAAATCCTTCATGAGTTGTTCTCCTTGATATACCTAAATTTAAGATTTATATAATAATTTTAAATTATTTGATTACTATTTCTGTAATTTCCGACTTATTTCTTTACTATTTTTTAGTTAAAAAATTAAATTTAATAATTTAATTAACATATTTAACATATTTTTTTTAACCTTAAATTTAAATATATTTGTAAAAAAATAAAAAAATTTGAACTTCATTTTAATAAAAGAGTATTATTAATAAAAAGAAACGCAAACATGCGCATATATTTCGATATATGGCATTTTTATTTTATTGCTAAAAAGAAAGGGGGATAATTATGAAAAACAAAGAAATAGATGCTAAACAAGCAAAACAAGAAAGAGCAGAAAAATTATTTGGTCAAACACCAATAAAAAAAGCAATTTGAATTGTTGCTATTCCAAGTTTGCTTGCTGCAATCATGGCCGGGCTATATGGATTTATTGACCAAATTTTTATTTTGAATTTGGTTCCAAAAAATAGAAACATCTTTGGAAATAATGAAAGTGAAATTGTTCAATTTTTAAATCCTGCCTTGCATAGTGGTGGATTTTTTAGTGATTATAAGATAATGATTGAACAATATAATTTACATTCAATTACGAAATTAGCTGAAATTACTCCTAATAGTATTGTTTCAACTGCGTCAGCAGTATTTGGCCCATTAATATTATTTTCTAATGCAATAGTATTTTTAGTACCTGTTGGAGCTTCAATTTATTACACTAAATGTGTAAGTAAGCAACTAAAACGGGCTGGCCAAGATTTATGGGCGACTATGTTTTGGTTTACGGTTGCGCTTTCTTTATTTGCTACGTTAGTATCTTTTGTGTTTATATGAAGTGGATTACTAAATGTATTAGCAGGTGAAACTCATATTGATTATAATATTGCTAGAATAGCCGGCATAGATCCTAATGCTTTGCAAGATTATTACAGCGCTGCATATTCATTAAGCATAAAATGAGCAAAGCAATTTTTATATATTTATGCATCAGGAACAATATTACAAGGATTAACTCTCTATCTATCTTATTTTATAAGGGCAGAAGGCTATAACAGTTATGTAATGATTTGTGCTATAGTTGCAAACGTAATAAATGTTGCTTGTGATGCATTATTTATTATTTGCTTTAAAATGGGAGTTTTGGGTGGTGTTATGGCAACCATTGTAGGTTGAACATTTAACACAATTACTTATTTTGTTTATATCTCAATAAAGATTAAACAAAATAAATCCTGGTTATCTCTTTCTCACCTATTCAAATTTAAATTTAAAAAGGAATTATTGGGACCAACATTTTTACTTGGCGCAAGTGGATTTTTAAGAATTTTTGGAATAAGTTTTTCATTTTTAATTATGAATATTCTAATTACAAAAACTTCATTTTCAATGCCTGATTACTTCCAATATTATTGAGCAAAAACCCAACCAATTATTATGTTGTTCTTGACATCAATATTTGGTATAAATGATGGCGCTAGAAGCTTATTTACATATAATTATTCACTAAGAAACATGGATAGATGTAAACAAGTATATAAATGAACTATATTGATAGCTATTCTATATTCAGTTCTTGTTTATATATTTATTGCCTTAACATCTAATAATTTGTGAATATGAGTTTTAAACATAGATCCTGATAGAATTGCAGGAACTAGTGTATTTATTAAGGTTATGTCGCTTAGAATAATTGCCGTTTCATTTGTTGTTGTTTCATTGCTAGCATTTCAAGGTTCAAATGATATTGCAAAGTCTATCTTTGCCTCAATATTTGAAAACTTTATTTGCTTCGTAATAATTGTTCCTATTGGATATGGAGTTGCTTGAGGAATATTTAATCATGATTCTGCTAACAGAGTAATTTCAAATTGAATCATTATTGCTGTGTTTGTTTTCAACTGTGTATTTGCTTCATCAATATTGATGTTTTATTCATGATGATTTGTCTATAAGAAAATGCCTTATATCGATCAAGCAAAAGTTAGTTGAAGTAGAAGAATTGAACATAAGTTTTTTGAACACGCAGAAAAACAAGAAGAACTATACAATGCTCAAATGCAACAAGAACAAGTTAATGAAAGTAATAATTTAAAGCAATAAAAACTAATAAGAATTTATATAAAAAAACTTGCGAATTTGCAAGTTTTTTATTTCTTATCGTTAAACAATTGCTTTAGTTTTATTGTCTTAAATTTTGTTGTTGGGCTGGGTGTGAATTGTTTTTACTATTATAAGTTTTTACTGCAAAACATAATAATGCAATTAAAACACCTAATTGTGCAAGATTAGCAATTACTGAAAGTCCATATATTGCTATGGCAGTTTTAGCAGCACCTGATAAAAACATTGCTGTAGCAACAATTGCTAAGACGAATGAAATTATAAACAAGGCAACTAACGAAATTGATAATTTTCTTGTTGTTATTTCATTTTTACTTAAGCCGCGAGATCCTGTTTTAAATCCATAAACAACTGTTAGAATAAAAAAGACGATGCTTGCTATTGCTAATAATAATCCAAATACTGGTACAAGCGAACCACAAATAATATAAATAACATCTATTCCTAATAAGATTGCTGAAAATATTATTAGAAGTTTTGCATTTTCTTTGGTTTTCAAGTTTTTTGAATTATTTTCCATTACTTATTTTCCTTTCGTTTTATATAAAGCATAATAATTATATAAAATTCATAATTTTTAAATATTTTAAAAATAAAACATCAAGCGCCCCAAAACTAAATTTTGTTTTAAATTCGTGTTGCTGCTTGATGTTTTTAAATAATTTTGTAATTTTTAGCTTTTATTGTTGATTGTCGGAATTTCAATTTTGGGGGCCTTGAGAGCTTCCATCATAGTTATTTGTTTCATTATTAACATTATTGTTATTTTTAGCGCAATAGACCTTAACTGCAAAACATAATAATGCAATTAAAACGCCTAATTCTGCAAGGATCAAAATTAAACAAATAATATATAGTGCAAATGTTGATGACTCTAATAAAAATGGATTATATTCATTTGTTGGGTATTCATAATTATATGCGCCAAGTGATAAAGATATTGCTATTATTATTGCAACAATAACCATTGCAATTGCTGCTGCAAACAATGCTATTAATGCAATGGATAATTTTCTTATTATCATTCGATTACTATTTTTTGGTTCACGTGATCCTGTTTTAAATCCATAAACAACTGTTAAAATAAAAAACACAATGCTTGCTATTGCTAATAATAATCCAAATATTGGCACAAGCGAACCACTAATAATATAAATAACATCTATTCCTAATAAGATTGATGAAAATATTATTAGAAGTTTTGCATTTTCTTTAGTTTTTAAGTTATTATTCACTTCATTCTCCTATTTTATAGCTTAGATATTTTTTAATTATATTAAAAATTAGCGTTTTAAAATTCATTTTTTTCAAAAGCATAAAAAAAGTTTGCAAATATTATTGCAAACTTCTTATTTATGAATTAATTATTTGTTTGTAATTCAATAACATTATTATTTGAAACTGTTAATAAGGCAACGTCACCTTCAACTAAATTTCCTTTTATGATTTCCATTGCTAATTTGTTTTCAATGTTTTTTTGAATATATCTTTTAATTGGACGAGCGCCAAAAGCGGAATCGTAAGCGTTATCAGCAACGAATTTAACTATTTTGTCATCAAAATTAATTACTAAATTATGGTTATTAATAACTCTTTTCTTTAATTTTGATAATTCTAGTTTTACAATATCGCAAACAACGGCAGGACTTAATGGGTTGAATGGCACTATTTCATCGATTCTATTAATAAATTCTGGTCTAAAACTTCTTAATAATAATGCCCTTACCCCATCAATGGTTAAACTCTTATTTATAATTTCACTACTTCCAATGTTTGAAGTCATGATAATTATTGTGTTTTTAAAGTTTACTACCCTTCCTGTACTATCTGTTAAGGTACCATTATCCAATATTTGTAACAATATATTTAAAACATCTGAATTTGCTTTTTCAATTTCATCAAATAAAACTATTGAGTAAGGATTTTGTCTAATTTTTTCAGTTAGTTGTCCCCCATTATCATAACCAATATAACCTGGAGGCGATCCAATTAATTTTGACACGCTGTGTTTTTCCATATATTCAGACATGTCTAATCTTACAATGTGATTTTCATCATCAAATAAAGCTTCTGCTAAACTTTTTGCAAGTTCAGTTTTACCAACACCAGTTGGCCCTAGAAATAGGAACGAACCAATTGGTCTATTTGGGTCATTGATGTTTGCTTTAGAACGCTTGATTGCATCAGAGATTAGTTCAATTGCTTGATCTTGACCTTTCACTCTCTTCTTTAATGTTTCAGACAAATTCAAAATTTTTTGTTTTTGGTTTTCTAGTAATCTATTAACTGGAATATTTGTTCATTTTGAAACAATATCTGCAACTTCTTCAGCATCAACAACTTCCTTGATTAAACGATTTTTCCTTGATTGTATTTTTTGTTCCAATTCATCTCTAGTTTTAATTAATTCAGGAACTAAAACATACATAATTTTAGAAGCTTCAACATATTTTCCTTCACTTTGTAGTAAAGGCAATTTTCTATTCAATTCTTCAATATTTTCTTTAATTGAAGAAAATTTTTGAATATCTTCTTTTTCTTGCTTTCAGCGCTTTTCAAGGTCAACTTTTTGCAATTTTAAAGTATTTAGTTCTTTATCTATTTCTAAAATTCTTTCTTTATTTTTAATGGCTGTTTGCTTTGTTTCACTATCATTTGTTAGAGCAGCTTTTTCAATTTCTAATCTAGCAATTTGATAATTAATTTTTTCAAGAGGTTCAGGCAAATAGTTCATTTCAGTTTTTATATTTGATGCTGCTTCATCAACTAAATCTATTGCTTTATCAGGCAAAAATCTATCTGCAATATATCTTGCTGATAAATTTGCTGCCGAAACTATAGCAGAGTCATCAATTTTAACTTCATGATATGCTTCAAATCTTTCTTTTATACCACGCAATATAGTAATGGTATCTTCAATTGAAGGTTCTGAAATTAAAATCTTTTGCATTCTTCTTTCAAGGGCTGGATCTTTTTCAATATATTTTCTATATTCATCCAATGTTGTTGCCCTAATTAGATGCAATTGTCCTCTTGCCATCATAGGTTTCAATATATTCGCCGCATCCATATTGCTATCGCCAGTTCCTCCGGCGCCAATTATCATATGAATTTCATCAATGAAAAGAATGATTTCACCATTTGCTTCATCAATCTTTTTCAATACTTTTTTTAGTCTTTCTTCAAATTGACCACGATATTGTGTTCCTGCAACCAATGCAGGTAAATCAATTTCAATTAATTGTTTACCTTTTAGGTTTTCTGGAACTTGATTTTCAACAATTTTCCTTGCTAGGCCTTCAACAATTGCTGTTTTACCTACGCCGGGTTCGCCAACTAAAACGGGATTATTTTTAGTTTTTCTTGACAAAATTCTAACTAGTGATCTTATTTCTTCATCACGGTTTATAACAGGTTCTAGTTTGTTCTGCGTAGCTAACTCAGTTAAGTTTCGACCAAATTCCTTCAAGGGATCTTTTTCTTGTTCATAAGGTGTATAACTTGCTTCCATATATTGTCCTCCTTTTACAATAAAAAATTATACTACAAAAATTAGCACTGTGTTAAAAAGAGTGCTAATTTTTTAATTGATATGTTGCCCATAAAAAGATTTGCTATTAAAATAATTTTATTTTTTTATAAAAAAATATTTTAGTGAAACATTATGTTTTTTTATTCTATAATTAATCCGGTTATTTCGTAACCGATTGAGTTAAGATGAAAGACATTATAAATAAACGATTTTGATTTCTATTTTTATTTATAAATGTTGCTACGCTTTGCATTTTTTTAGGAATTGCTTATCTAAACTGAGCTCTACTAACAGGATATTTGGTAGGAGTAATCAGTTTTCTTATTTTTTTAAGTGGCTTACATTTAGTATTTAAAAAAATGAATGATTGAAAAGAAAACGCTAGTATTAAAAAAAACAAAAACCTCGCAGTTATTATTTTTTTAATTTTAAATTTTCTTGCTTTATTGATTATTGCCTTATTTGTAATCTTTAACTTACTTTATAAAAACAAACATTCAAATGCAAATGTTGCATTTGTACCTTTTAACGTAATCACAATGGCTATTCCATATACATTATTTAGTTTTCAAATAATAGTTGTGGAAGTAATTAAAAAAATTACCACTAAAAGGAATTTAAAGAGAAGGGAGGAAAATGGATAGAATTTTACGTCTTCATTGATTTGGTGGTCCAGTTAATCCTGGCGAAGTAAGCAGTTTCAATGACAACCAATTATTTACTTTAATTATTTTAGTTGCTTTAATTTTTATTATTTCAATACTAATTTTTGTAGCAATAAAAAGACAAAAAGTTGATAAAGCACCTAATACAGTTGTATTAATGGTTGAAAGTATAATAAAAAACAGTGATGAATTTATTTCTGAAACCCATGGCAATAAATTTGACAAAATGAATCCTTATTTTATTTCATTATTTGCCTTCTTGATTTTAGGAAATATGCTTTCATTAGTTGGTTTAGCCCCAATTGGAACTAGTTTGTCAGCAATTTTTGCTGCCACTGTTATAAGTTGACTTGGAACACACATCATTGGATTCATATATAAAAAATTTAAGTATTTATTTAAAATTCTTAATCCCTTAGAAGCATCATCAAACTTTTCACCATTAATAAGTTTAACTTTCCGTATGTATGGAAACATACTTGGAGGAGTTGTATTGGTTGCACTAGTAAGTTTATTCTTAAATAATATTTGAACCAAAATTATTGGCGCTAACTTAGAATCACCTTCAGCTGAGTTAAACCCTATTGGGTTATTGATCTTGCCAGCATTTAATTTATATTTTGATATTTTTGATAGTTTCATTCAAGCATTTGTATTTATGATTTTAACAATTTCATATTGAACGCAATCTGCTGAAGAAGATATAAAAGAAAAACATAAAGACAAAATAAAAGAATGAAAACAAGAAATCTTAAAAAATAAACAAAATAAAAATAATATAGATAATAAAAATAATGAAACCTCAGTAGTAGAGGCAACTATTTAGGAAAGGAAAAAAAATGGATAAATTAGAAAATATTGTTAATGCATTCAATACAGCATCAGCAGGTGTTAATAGTAAAGAAGCAGGAGTAAACTACCCTCTAGCATATGGTTTAACAATGTTAGGAGCAGGTATTGCTGTTCTTGGCTCAGGTTTAGTTTCTATTGGTCAAGGTTTAGCCGTTGCTAAAGCCTGTGAAGCTGTTGGAAGAAATCCAGAAGCATTTTCAAAAATTCGTACATTGTTAATTTTGGGTTTAGGTATTGTTGAAACCGGATCTATTTATTGTTTAGTTATTTCATTATTGCTAATCTTCGTTTAAAAATATGTTTCAAAATTTTGCAAATTTATTAAGCCAATCGCAAAATGCAATTGCAGCAGCTAAGCCATCTGCTTCTGCTAAAATTGCAGAAGAACTAAACAAAACTTTTAATGGTTTAACATTCAATTGACCATTCTTTGTTTTTTCATTAATAAGCTTAGGCCTAGTTGTTTTGATTATTACTTTATTAGTATATAAACCAATAAAGAAATTAATTAAAAATCGCCAAGAACTTATTCAAAAAAATATTGATACTTCAATATTAGCAAAAGAAAATGCTTTAAAAGTTCAAGAAGAAAATGATAAAAAAATTATTGAAGCTAATAATCAAGCAGCTTTAATAATTAATCATGCTAAATCAGAAAGTGAAAGAATTGTCAATGCAAGCACGATTGCTGCTAAAAAACAATCTGAACTTTTATTAGATCAAGCACAAATTTTGATTAATAAAAAGCAATCTGAGCTAGAACAAGCACAAAAGAAAATAATAGTAGAAAATGCTGTTGAATTAGCCAAGAAAATTATTGGCCGTGAAATTAGAAATGAAGATAACATTAAATTAATTAATGAAGTCTTAGAAGATTAATATGCAAGATTTTAATGAAATCATTTATAACTGAGCATATGCAATATATGATCTTACAAATGAAAACAAAAATTTGGCATTAGTTTCTAATGAAGCTGCTGAAATTACAAGACTTTTAAAGAAAAATAAAGATTATTTGTATGTTTTAAATTCTTATGATATTGACGAAAATGAAAAATATCGTCTAATTGATGAAGCTTTTTCAAGATACAATTTTTATTTAGTAAATACTCTTAAATTAGCAGCTAAGCAACATGTGATTAAATACATTATTGTAATATTAACAAAATTTGTTGAACTTTCAAATGAAAAAAGAAACATTAAATACGGGTTTGTTTATACAATATCGCCTTTATCTGAAAGAGAATTAAAAAGGTTAGAAGTAAAACTTTCAAACGAGTTAAAGGCTAATATTCATTTAACAAATGAAATTGATCCCAAATTAATAGCGGGAATCAAAATAAAAGTTGACGATTATTTAATCGATAATTCAATAGTCAACAAATTAAATGACTTTAAAAAAGTCGTAGAAAATAATTAAGGAGGGAGGAATATGTCATTAGAACCAAATGATTTGTCAGCAATCATTAAGGCGCAAATAAAACACTTTAATGAAGACATATCTAAACAAGAAATTGGCCGTGTAATAACAGTTGGTGATGGTATTGCCTTAGTAAGTGGCTTGAGCAATGTAGAATATGGCGAATTGGTAAAATTCGAATCCGGCGTTATTGGAATGGCCTTGAATCTTGAAGAAGATTTAGTTGGTATAGTTGTCATGGGTGATGACCGTAACATTGTTGAAAATAGTACAGTTTATCGTACTAAACAAGTTATATCAACACCTGTTGGTAACCATTTATCTGGCCGTGTGTTAGATGCACTTGCTAACCCAATTGATGGAAAAGGTCCAATTCAATATGAATATACTAGACCAATTTTTAAGATTGCACCTGGTGTTATGACTAGAAAAGAAGTTAATCAACCACTTGAAACCGGGATTTTGGCAATTGATGCTATGATTCCTATTGGTAAAGGTCAACGTGAATTGATTATTGGCGATAGACAAACAGGCAAGACTGCTATAGCCATTGATGCAATTTTAAATCAAAAAGGTCGCAACGTTTATTGTATTTATGTTGCTGTAGGTCAAAAGAATTCAACTATTGCACAAATAGTTGATCAATTAGCAAAACATGATGCATTAAAATATACAACAATTATTTCTGCATCAGCATCAGACAGTGCTCCTTTACAATATATTGCACCATATACAGGAATTACTATTGCCGAAGAATGAATGGCAAGAGGTAAGGATGTTTTAATTATATATGACGATTTGTCAAAGCATGCTGTTGCATATCGTACATTATCATTACTATTAAGAAGACCACCTGGACGTGAAGCTTATCCTGGAGACGTCTTTTATATTCACTCACAACTTCTAGAACGTGCAGCTAGAGTAAATTCTGATTATGGAGGCGGTTCAATTACTGCTTTGCCAATTATTGAAACTCAAGCTGAAGATATTGCAGCATATATTCCAACAAATGTTATTTCGATAACTGATGGTCAAATCTTTACTAAGGAAGCATTGTTTAACTCTGGCCAACGTCCTGCTATTGATATCGGTTATTCAGTTAGCCGTGTTGGATCAGCAGCACAAACAAAATTAACAAAAAAGGTTGTATCAAGTCTAAAATTGGAATTAGCACAATACAATGAAATGTTAGCATTTGCTCAATTTGGTAGTGATCTAGACCAAGCAACTAGAACTGTTTTGGATCACGGTGCTAAAGTGTATGAATTATTGAAACAACCTCAATATTCTCCATATTCTTCAATAGAACAAATAATTATTCTATTTTGCACAAAATATCGTTTAATAAATGTAATTCCAAAAGAACAAATATTAAAATATAAAACTGATATTTTAAATTACTTTAGAACTAATCCTAATGCTATTAATACATTAAGAGCCGTTTCTGAAGCAAATGATTGAACTAACGAATTAGTTGAAAAACTATATAATGAAATAATTAGCTTTAATGAATTATTTGTTAAAACTATTCCATTATATAATAAAGATTTATATCAACCAGTACCTGAATTACCATGGAAAGTTTGCAAAAAATAAAACATAGAATTAATTCGGTTAATTCAACTAAAAAAATTACCAAAGCAATGGAGCTAGTTGCAACTGCTAAGTTTGCTAAAATTAAAAATCAATTTGAAAATATCAATAATTATTATCAAAGTGTTGAAAGAGTTTTTTTAAACCTAATTAAAAATTCTGATCAAAATATTGATAAATTATTGAACAGCACTTCTTGAAAATTCAAAAGCAAAAGAAATTTATATATTGTATTTGGCAGTGATTTAGGACTATGTGGCCCATACAATTCAGAAATGTATAAAAAAATTACTCAAACTGTTGGAAAAGAAGATATCCTAATAGTAGTTGGTAGCAAATTATTGAGTTTAATTCAAAAAGATAATAGCCATCATATTATTCAAATATTAACTCAAATTGGTGACAATCCAAGTTACGAAATCGCTCAAATATTGTCAAATAAGATTTATCATGTTTTAGAAATATCATGGCTAAAATCTATTAAACTTATTTATACTCAATACATTAATCCAATAAAATCCGAACCAATAGTAAAAGAAATTTTTCCAATTTCTATTGATACACTATCAAATAATGATAACAATTTAGCAGGGGAAGAAAGTTCAAAAAGTATTGATCCTTCTTGCTTTGAACCAAACCCTACTGTAATATTGCAAAATTCATTTTCAATATTCTTTGAAGCCTCTATATACAATGCCTTAGCAAATGCTAAGCTTGCAGAAATGAGCCAAAGAAGAACGGCAATGGAACAAGCAAGTGATAATGCACAAGATCTAATTAATGAATTAAATGTTCAATATAATAGTTCTCGTCAAGCAAAAATTACACAAGAAATTACTGAAATAATTAATGGATCAAATTAAAAAGAAAGGAGTAAAAATGGCAAATAAAAAAACTAATGAAAATCAAGAAATTCAAGAAGAACAAAACCCAGTTGATGAATCTAAAAATTCAGAAAATTATGGCGTTATTAGCCAAATTTTGGGATCTGTTATTGACGTTAGATTTGAAGAAGGACATACTCCAAAAATCTTAAATGCCTTAAAAATTATTGATGAAGACAAAAAATTTAGCAATGAACAAATTCTTGAAGTTGCTCAACACGTAGGAAATGATACAGTTAGAACAATAGCAATGAACTTGACTATTGGTTTAAAGCGTGGTATGAAGGTTCTTGATTTGGGTTCTCCAATAAAGGCGCCTGTTGGTAAAGAAGTTCTATCTAGAATGTTTAATGTTCTTGGTGAACCTATAGACCAAAAAGGTGGTGTATTCAAACACAAAATGCCTATCCATAGAGCAGCGCCAAGCTACGAAGAACAAAAATCAACACTTGAAATATTTGAAACAGGAATTAAAGTTATTGACTTACTTGTTCCATATATTAAAGGTGGAAAGATAGGTTTATTCGGAGGAGCCGGAGTTGGTAAAACTGTTTTAATTCAAGAATTAATTAACAATATTGCAAAACAACACGGCGGTCTTTCTGTGTTTGCAGGTGTTGGAGAACGTACAAGAGAAGGTAATGACCTTTATCACGAAATGCAAGCAAGTGGTGTTCTAGAAAAAACTGCTCTAGTATTCGGTCAAATGAACGAACCTCCTGGAGCCAGAATGAGAGCACCATTTACTGCTTTAACTATCGCTGAATACTTTAGAGATGTTATGCATCAAGATGTGCTATTGTTTATTGATAACATATTCAGATTTACACAAGCAGGCTCTGAAGTTTCTGCATTGTTAGGCCGTATGCCTTCTGCTGTTGGATATCAACCAACATTGGCTGTTGAAATGGGTCAATTACAAGAAAGAATTACCTCAACCAATAACGGATCAATTACCTCTGTTCAAGCTGTTTATGTTCCTGCCGATGACTTAACAGACCCCGCACCAGCAACAACATTTACACACCTTGATGCAAAAACTGTTTTGGACCGTGATATTGCTGCTTTAGGTATTTACCCTGCTATCGATCCTTTGGCTTCATCATCAAGAATGGTCGATCCAAATATAATTGGCCTTGAACACTATAACACTGCTAGAGCAGTTCAAAATATTCTTCAAAGATTTAAGGAATTACAAGATATTATTGCTATATTAGGTATGCATGAATTAAGTGAAGAAGATAAGAAAGTTGTTGCAAGAGCAAGAAGAATAAGAAACTTCTTATCTCAACCTTTCTTTGTTGCCGAAAAATTCTCTGGTATTAAAGGACAATACATAAAATTGTCAGATACTATTAGATCATTTAAAGAAATTTTAAGTGGAAAATATGACGAATTGCCAGAAGAAGCATTCCTTTATGTGGGTACCATTGATGATGTTATAGAAAAAGCTAAAAGACTTGGGGTTAATGTTGAAAAATAATGGAAACTCCTAAGATACATATTATTATTAGTACTCCATTAGGAATATACTTTGATAAAGAAGCAAGTATTTGTACATTTACTACTACTGAAGGGCAAATTGGAATTATGTCAGGCGCAACTGAGTTTATGGCAGCCTTGGTTCCTTCAAAAATAATGGTTAATTTTTTAAATTCACCTGATTCTAAAGTTTTTTATATTGATAAGGGAATTGTTCAATTTAAAAATAATGTCTTATCAATAATAGTTAATCAAATTGATACTAAAGAAATTGAATTGGATCATAAATTTAGCCCTGAAGATCAAAAGAAATATACAATTATTGAAGAAGTTACTTTGAAAAAGAGCATTTTGAAAAGTCAAACAAACAATAAATAATTAATTTTAAAAAAGTAGGAATTTAAAATCCTGCTTTTTTTATGCTAATTTTGAAACTAAATTTTTATAATGAAAAATTTATCAAATTAATAGTTTGATTAAAAAGAAAAATTTCTGATTTCAATTTTTAAATTTTAAAATATTTCGGCCATTTTTTATATGTTTAAAATTCTTAAAATTTGTAATTCTTTTTATAAATTTTAGAATTATAATTCCACAGTACAACAAAAAACAAATAAGGAAGGAAGATAATAAAATGAAAAAAACAGCAAAAGCTTTATTAATAATATGCACAACTATAATACCTAGTTTTGCTATGCCATTAATATCGGCGGGATGTAATAAAACAACAAACCATGAAACAAACAAAGAAAATACAATTATAGATAAACAGGCCCCTATTAAAAAACAAGAAAATGATTCTAATAACCCCAATAAATTAGAATCCAACAGTGAATTAGCAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAAATTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAATAACCTTGATAAAAAATTTAAAATAGAAATTTTAAAATTAGAAGCAGACAAGAAGAATAATTTAATATCGATCTCGTATAATATTTCGTTTAATGATGGAAATAAAATCACTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAATAACGAAAGCTATTTAAATGGATTATTGAATGATAATGACACAATATTTAATTTAACAACTAATTTCAAAAAAGGTAATGAAATAGATGCAAGCCAACTGGATGCATATCATTTAGCTAATGGCCAAGGTTCTATTAGTTTATTGGCATCTAATAAAAATGTTTATGTTCCCAAAGATCTAATCTTAGAATATACTTGAAATGAACAAAATGAAACAAATAAAAAATTCAATGGTGAAGTAGAACTTGATGTTAGTGTTAAGACTAAGGACAATAAAACTATTACAAGAAAAAATGTAAAAATCGCTAATAAGATTGCTGAAGGCGTTTCAGATGATTATTTAACTAAATATAAAAAGTTAACTTTAAATTATAAAGACGCTGACAAGACTTCAATAAATTCTTTCAAAGAAGGTGATATTGAACAAACATTAAAAAATAATTTTATTGCCAAATTTGAAAAGAGTAGTGAAGAAGAAACTAAAAAATTCCTAGAAGACAATGGAATTAAATATGAAGTTAAGAGCATAACTGAAATTTCTCTTGAAAAGAAAACAATCAATGTTAATCTTCAAATAACATTAAAAAATAAGAAACTTGTCAATGCAAATATTGAAGTTGTGGGATTTAAAGGCGTTGAAAAAGTAAGCGATGAACAAGTTAAAAAAATAGTAGATAAATTGACCTTTGGTGATGTTTATTTCCCTGAAAAAGTTAAAACCTTTAATCAAAGCCGTTTAAACAAATTAAATTTATATATTAAAGATGAAAACGGAAAAGCTCAACTAGAAAATTCTATAGATTTTGCAGAATATTTTACAAAAAATGGAATTAACAATTTTAATAAAAATTATATAGTTAAATATGATAAAGCCGAAAAGAAATTAATTACAACATTAAAATTGAATGAAAAAGAATACACAATTGAAACAAAATTAACGCATGAATCAAAACCAGATGAATGGGTCAACGAAGTAGGTAAGGAATTTTTAGGAAATCCAAATGAAATTTTGGCAAGTGATTTCTCTGCTACAAATTTAAAATTGAATAAAAATGTTGGCGATAGCCTTGATAGTTTAAATTTTATTAAATTGTCTTCAATAAAAAGACCATCAAAAAATTCAGAGGAATTAAAACTCAACGAAGAAGGTAAGGCAACGTTAATTTATGAATTTAGAAATATATATGATAATTCAATATATACAAAAGAAGTCGTATTTGAAAATCTAAAAAAATTAGAAAAAGCTGATTATAAAAAACAACACGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTAAAAGCGGTTGGGCTACTGACGAAGAAATAAAGAGATTGGAAACTTTTTTGCCAGGCGAAAAAAAATCTAAAAAAAATAAGAGAATAAACATTGAACATTATATAAATAATGGCAATACAGAAATACTTGTTGGAAGAGTAATTGCTAAAAAAAATAAAGTTACTTTCGGCGATACATTGAACAGACAAAAGGAATTTGAAGAAATAATATTTGATGAAGAATTTATTGAAGCAGTTAAGAATTCGTCAGATAATAAAAGCGAAAATAAAATATTATCTAATGTAATAACAGAAGAAAACTCTAAACATAAAAATCAAAAGAATTGAATAAGGCTTACAAAACATGAAGGAAAATGATGTATATTATTTACATTCATTGAAGATACAAAGTCAACGCCATATTATGTTCAAATAGAAAAATAGTCTTATAAAATTTTATTGCAATAATAAACGCAATAAAAACATTTAAAGAGTAGGGGACTGGCCCTACTTTTTATATTAAAATAAAATAGTAAAAACATAAAAAATAAAGTCAAAAGTTTTTATTTTTAACCAAGGAGTTTAAAATATTTGTTTTTTGTTAATTTTTAAGATGAATGCTCATTTTTAATCACCTTAGCAAATAAAATTATTTTAAAATCAAAAAAAGGAAATACAGAATATGAAGAAATCGAAAAAAATTTTGTTGACCATGGGAGGGATACTTCCCTTGGTTATTTCAGCGCCATTAGTTGCTGCTGGATGCAAGGAGCCAAATAAACCAGAAGTTAAACCAGAGGATCCAAAGAAACCAGAAGGTGAAAAACCCGGAACAACTCCCGAAGTTAAGCCAGAATCTAACAGTGATTTAAATGATTTAACAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAGGTTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAACAACTTCGATAAAAAATTCAAAGTAGAAGTTTTGAAATTAGAAGTAGATAAGAAAATTAATTCAGTATTAATTACATACAACATTTCGTTCAATGACGGAAGTAAAGACGCTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAACAATGAAAGCTATTTAAATGGATTATTAAACGATAAGGATACGGTATTCAATTTAGGTGATGCTTCTAATGAAGATAGTTTAACAACAGTTGAACAGTTAGATGCATATTACTTAGTTGATGGTCGAGGTTCTATTAGTTTATTAGCATCTAATAAAAATGTTTATGTTCCTGAAGGTCTAATGCTGGAATATACTTGAAATGAACAAAACGAAACAAATAAAAAATTCAATGGTGAAGTAGAACTTGATGTTAGTGTTAAGACTAAAGACAATAAAACTATTACAAGAAAAAATGTAAAAATCGCTAATAAAATTGCTGAAGGCGTTTCAGATGATTATTTAACTAAATATAAAAAGTTAACTTTAAATTATAAAGACGCTGACAAGACTTCAATAAATTCTTTCAAAGAAGGTGATATTGAACAAACATTAAAAAATAATTTTATTGCCAAATTTGAAAAGAGTAGTGAAGAAGAAACTAAAAAATTCCTAGAAGACAATGGAATTAAATATGAAGTTAAGAGCATAACTGAAATTTCTCTTGAAAAGAAAACAATCAACGTTAATCTTCAAATAACATTGAAAAATAAGAAACTTGCCAATGCAAATATTGAAGTTTCAGGATTTAAAGGCGTTGAAAAAGTAAGTGATGAACAAGTTAAAAAAATAGTAGATAAATTGACCTTTGGTGATGTTTATTTCCCTGAAAAAATAAAAGAAATTTCAAAAGACGAAACATTTTTGAATGAGTTGCCTTTATATATCAAAGATGAACATCAAAAAATTAATCCCGAAAAATTTGTTGATTCTGAATATTTAATTAAAAATGGGATCACCGAATTTGATAAAAATGAATCTGGGCAACTTAACTACAAAGTCAAATATGATGAAAATGCAAAAAAATTGTCTGTAACATTAAAATTAAACGGCAAGGAATATAATGTTGAGACAACATTAACTCATGAATTAAAATTAGATGATTGGGTAAATGAAGTTGGAAAAGAAATTAATGGCGATTCAAATACAATTCCTGTAAATGAATTTTCTAATAAAAATTTAAAATTGAATAAAAAAAATAATGACTTTATCAATGGTTTAGATTTTATTAAATTAAATTCAATAAAAAGAGCAAAATCTTCTGAAGCAGAAAAAAAATTAAATTCAGAAGGTAAGGCAACATTAATTTATGAATTTAGAAATATATATGATAATTCAATATATACAAAAGAAGTCATATTTGAAAATCTAAAAAAAGCAGAAAAAGTTGATTACAAAAAACAACATGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTACTAATAATAAATGAGCTTTGCAAGAAGAAATAAACAAAATAAGTGCCTTTAAAAGCAATAAAGAAACCTATTCTTATAAAGATAATGAAGGTAAAGAACAAAGTGAAACATTATCTGGTAGATTTCAAATTAAAGGAAAATCAGGCAATTTAGAATTGCATTTTGGGGATAATGGTAAAGCTCGCGAATCATTTAAAGAGTTAAAATTTGACAATGATTTTCTTGAAGCAATAAAAAGTAAAACATTAGTTAATGTTGTTAAGGATTCTAAACACGACCAATGAGTTAGATTTACACAACACAACGGCAAATGGTGTATAGTATTTGCTTTTAAAGACGATCCGGAACTTACACCTTACTATGTTCAAATAGAACAATAATAAGTTTAAAAATAAAGTTCATTCTATTAAAAGAAAGGACTTTATTTTTTATTTATAAAATAAAATTTATATATTATAATAAATTATTACAAAAAGCAAAAGGTGGGCAAAATGACTAAGAAAAAAGTATTATTTAAAATATTTGCTATGGGTGCTATTTTAGCCGGAGGCGTTTTTAGTTTAAATTCATGTATTTTTAAATCAAATGATACAGAAAATAAAGATAATAGTTTTGAAAATAACACTAATAACGGTCAAAGGAACCAACATGATTTAAGCACCGAAGATAATAATTCAAGAGATTTGATAGTTCCTGAATCAAATAATAAGCCCGAAAATGATTCTATCCCTCAACCTGAATCAAAACCAAATATTATCCCAACGCCTGAAATAACGCCCAAACCAGCGCCTGAAATAACGCCCGAACCAACGCCCAAACCAGCGCCTGAAATAACGCCCAAGCCAAATGAAAATGACTATAATAAAGCCAATCATAATCAAACTCATTTTGTAAGGCCACCTTTTATTGCTAAATTTAAAATTAAAAATACTGATTTTTCAATAGCGACAATTTTTAACCATTTAGATAGTCCTGGTACTAGAAAAAAAGGAGAATACATTGAACCAAAGCCATCGATTTTATTGGCTGATAGTTCAAATAGACAGTCAGATTTGAATAAACAAGGAGCCCAAGAAGTAAGTGAATTTCTAGGCTTAATAGATGTTATTAATGAAAACAAAGATTTGGCCAATTTTGTTATTTACGGTGGCGATACTAATATAAAAAATGAAAACTTTTATTTAGCAAGAAAATATCCCGAAAATATTCAATTTACTTTAGATAGAGACTTAAAATTAAAAAAATATGACCAAAAATTTCTAACATCGTTAGGAACAAGTGGAAAATATTCAGAACAATACGACAAAATGTTCTTTATTAATCAATTGCCCAATACATTTAAACCAAAAATAATTACCAATGGTCTTTTACCTTATAAGGTTGATATTTATAGAACGTTTAATGATGTTATTTCAAAAGCAGAACTTGAAAACTTAAAAGGATGAAAATCAAAAAATAACGATAAATCTACAATTAGAAGTCAAATTTCAGACCATGCTCCAGTATTTACAGATGTCGTATTTTCAAATCCAATCGAAACGCTAAAAGTAAATGAAGCATTAAGAAAAAACCAAACTTTATCTAAAGAGCAAAACACTATAAGAATAGCTCATTGAAATATACTAAATTTTGGCGGTCCTAATCAAGATGCTAAAACAATGGCTATTGCTACTACAATTTATAAGTCTGGTTTTGATATAATAGGCTTAACTGAAATTAATGATGGAAGAGGAAACTATGTGCAAAATATAGTTAACCTTTTAAATGAATATGCAGGAAAAGATAGATATAAATTCGTATTACAAAATAAAAAGGATACAAATATAAGGCCTGAATATTTACAATCAAATAAGTTTGGAAAAGCGCAACAAGAACAAGTTGCAGTGATTTATGATAGTCAAAATTTTGAATTTGTTTCAAGCCGAAGCTATTTAAAACCAATTAAATACTACAAAGATAACTAAAAAAACTTGGATGCTTCCAAGTTTTTTTAGGCTAATAATCACCTTGTTTAATTTTATTTAATCCACTTCATTTGTTAATTGAAGTTTTGTTATTCATATTCATGAATTTTCTATAACCACCTTCTTTGATAGTCATTAGAATATATTCTTTTTTAGAAACTTCATTAGGTTTTCCGTTTTCTTTAACTTTGAAGTTTACATTTTTTAATTTACTACTTTCAGATTTGTTATATCCACTTGTTACAATTAAATAAGTAATTGATTCAGGGCCATAATTTTCTTCTTTATAGTATTCTCAAGAACTAAATAATCAGCCTCGAGTTTCGTTAAAAACATAGTTAGTAAATTCTCTTCTTTTTTCAGGGTCTTTAAAATAAGCCTGCATTCCCGTAAATTTACTTGATACTCCAGGAATTGCTTTAAGATTAGTTCCTTCTTGAAATTCTCAAAGTATTGCCCGTGTTCCTTGGTATAGGCTCAATGTTGGCGCATTTCATCTTGTACCGGGTTCCCTATCTTTTTCCAATGTTTCTCTACCAAACATTTCAAATATAGCAAAATCTACTGCTACATTAAAACGATATATTGCTTTTCATGCTTCAGATTGTGCCCTAACTTTATATGTTGGTGAAGTTGCATCCTTATCATTATTGTATGCCTTTGCAAGCAATTTATAAATTGTATCAAGCAATTTTAAAACATCTGCGGAATATTCAATATTATCAGGGTCATCTTTATCAGAGGCTTTTGAAGATTTGAAAATACTTCCTAACGCATTTGAAACAGAACTATTTAAGGTATTCATGTTTGAACGTTTTGATTCATCTGAATTATTGTTTAGATTTCTTAATGCATTATAGATTACAGAGTCATTAAAATTACTTTTTCCAAATACTAAATTGATTAATTTAACCAAAGCAGTTTCATTGATTGGTTTATCTTCACCTACTAATTCAGATAATACTAAAACATCATTAAAGCTAAATGCTGATTTAATTTTTTCAATTGCATCATTCAATCATTTAACAGGGTTTAAAATATCAAATTGTTTAGCATAATTACTTATCAATCCTATTGAACGTTTAATGAATTTCCTTTTAATAAAATCATTATTTAATAATGCTGGTACGATTTTTCCAATTAAGTCTTTTAGGAATTTTTTATCTTCATCGTTTCCTTCTATTTTTAGATAATTTAAAATACTTTCAACAATGTGATTTGTAATTTCATCATCAGTTGCAAGATGCTTAATAAGTTCAGAAGAGTTCTTAATTAGATTATCTTGAATTCTAGTAATTGTTTTAGATATTAAGTCCCCAAGGTTGCTTGATTCAATTTTTGGTCCCGCTAGAAAATCATTAACTACGGCTTCAATTAAGCTTTTAATTTCTGGAAAATCAATGAATTTTTTACTTATTAATTCTAACTTATCTTTTGGAATAATATTTTGTTTAAGGCCATCTCAATTAAATTTCTTAATTTGATCCTTCAAAACATTATATACAAAATCAACTAATGCTTCTTTTCCATTTTTATTATCTGCTTTTATTGATCCATTTAGAGCTTTTAAGGCATTGTAAATTAATTTTTCTAGATTCTTTAGGAATTGTTCGTGTTCCTTATCTTGTTTAGTTTTATCTACTTCGTTGAATGTTGTAGAATTGCTTTTAGCAGAAAATGCTTGTTTAAATTTATCAAGATTGTCAACTATATATGAATAAGCGAAATTCAATTCATTATACAAGACATTTTTATCAATATTTTTATCTAATATTGTTTCAAATAATTCACTTAAATTTTCTGTTGTTAAATAGTTATGGTATTCAATCTTTTTAATAGATTCAAGAAGTTTGGATGCCAAAGCATTTCCATTGAAATGACCATTATTGTCAAGGAAATCAACATTAACAAACAAATCAATGAAATCTCCCAATATATTACTAAATACGTTCATTGAACCTAGTTTGTTAATTAATCTAGTCAAAAGATTTGCAATTCCTTGAATTTGAGCGCTACTTGAACTAATTGAATATTTCTTGTTGAATAAATTCAATGCATATTCAATAGATTTGTTAATTAAGTTTTTGTTAGAAGTTATTTCATTTACTGCATTTTTAATAAATGCTTTAAATTTATCTTTATTTGCTGACAATAAATGTTTAAATAAACCATTGAAATCACTAATAGAAGCTCATTGATTGTTATCGCCTAAATCTATAAATGAATTTAATAATGTTAGTATAAATTCTTTTGTATTATTTGAATCAAAAATTACCTTCATTAATGTTTCAACTATTTCATTGCCTAATTCAGAAGCTTTTGAATCAACCTTTAGAATTTTTTCTTTTATTTTTAATAAATTGTGTTTGATATATTGTTTAAATTTATTACTTTGCAAGAAAATATCAACTGAATTTTTTAATTCATCTTTAATTTCTTTCTTTGCCTTATTGTCTAATATTGAAAATACACATTTAAACAAATTAAAATAAACATTGATATTTACCTTGATTTGTCCATCAAATCCAACTATATTTGTACTTGAAGAATCTAAATTTTTATGTTCAGGAAGTGATTGAGATTCATTGCTATAATCCGGAATAATATTTTCAAAGTTTTTGATATTTTTTAATAATAATAATGCAATATTTTTTATATCTTCAGATTTTAAAGATTTAACAAATTCTATGATTTTGTTGTAGTTTAAATTATTAATAATGCTTACAATATTAATTAAATTATTTCAATCGTACTTTTTAAATTCAAATTGATTCTTGTTTAGATTATTGACTGAGTAGTCTAAAACATTTTCAATTAAGCCATCTAGAATATTGAATTCTGGCAATTTTTCAATGCCTAGCGAAATTAATTTTAGTAATGTTTCTTTTGCATTAACAGGTCATGTAACATTCTTTAGATTTTGTTCCATCAATGAAATTAATAATTTTGCTATTGAATTTTTAATATCAATACCTAAGGTTTTGTTATTGATAAATTTATTGAATATTTCTTTTATTTTCTTAATAACATATTCTTTATTATTTGAAATAAATTTGCTTATTCATTGAGAAATATTATTTAAATCTTTTAAATTATTATCATCAAATAACTTTTTAATTAGTTCTTTTATTAAAGGCAAAAGATCTTCATTGTAACTTGTTTCAACAAATGATTTTATATATGAAATTATTTGTTGAAGGTTTTCTTGTACATCACCCTTAAAGCTTGAAATATTTATTTCATCAATGTATTTCAAAATAATTTCTTTAATATTTATTTTGCTTGCTATGTAATTTATAAATGTTTCTTTTTCTAAAACGATCTTTAATATTTTAATTAGTTCGACATCTTTTATTTGGTCAATTTCTTGTTCTTCATATTTCTTTGTTAAGAATTGTTGAACATCCTTAATTAATTCACCATTATTAAAACTAAAATAATTCTTAATCGTTTTTGTAACGGATTCAAGAATAGAAGCTCAAGAATATTTATTTATTGACTTCAATTCATTTGTTAATTTAATTTTTAAGTTTTTATATAAATTAGATTTCAATGAAGTAGGTAAAATCTTGTTGAAATAATTAGTTAATTGGTTAATTTCATCAGAATTCAATTTTGTTTTTGCTTCAATTTCTAAAAACGCAACTAAAAATTCGACTAATTTTTGGGTTATATCTTTATTTGATAAAACATTTTCTAGCAATTGTTCTAACGCATCAAAGCCTTTGTTGTCTGCAAGATTTTTCAATATTTTATTGATAAATTCAGGCAATGTTTGAGCATCATATTCACTGCCGTTTTCAATTAAATTTTCAATAAATTCGAAAACTTTGTCAATTAATTTATTATCAATAATCTTATCGAATTTAGTAACAATAAAATTGGCAAGATTATTATCATTTGTATTGACTTTATTTAGAACTTCATTATGAACATTTTTTAACTCATTATTAATTATTTTTGTTAAATATTGTTTTAAATTTTCATTTTTTAATAACTTTTTAACTTCTGGTTTTAACTCATTGATAATTGTAGATTTAGATGAAGCTAGTTTATTTCATAATTTTTTAATTAGTAAGAAGATTTGCTCAAAATTTAAATCATTGTCTAATTTAATTGGCTTTGAACCTTCATTTTGTTTTTGTGTTGTTGTGTTGTTGTTTGCTAAAACAGCATTTGCAAATTCATTTAAATTCTTGATTAATATCAAAAATAGAGTTTTTACATTTTCTTCATTTAATGTATCTATCAATGAATTTAATTGTTCGTAGTTAAATAAATGCTTAATTAGATTTTTTAGATCTAAAGCATTACTTCAACTAATTTTGTCTAAATTAAAACCATTATTTTCAATGTATTTAGAAATATACGAAGCGAATGTTGATACTAGCAAGTCAACATTTTGTTTTTGCAAGGCAAAATCAATCAATTTTGATACTAATTTTTCAATGTTGCTTTCACTATCTATTCCAGCAAGAAATTGAGGATATTGTTTCTTTAATAATTTAAATAAACCACCTTTAATTTCTTCGTTGCTTTTTATTTCTTTATCTAAGAAATTATTTAAAATTTCTTTTACTATTCCTACATTTTTTGAAATTACGTTTGCAACAAATTCATTAATGTTTTTAGATTCTATAACCTTATTATCTATTGCAGAAAGTTCAATAATTGATTTAATTTTTGGAAGTAGTTCGTTTTCTCATTGACTATCTATGTAATTTCTAGTTTTGGTGATTAAGGTTTGTAATAGTGAATTTAATTCAGTGTCGTTAGATAGTTTGTTGTCTTTAAATGAATCCAAGTAATTTAATATAATAGATTTAATGTCAAATTTTTTAAAAACGTAATTAACAAGTTCGGATTTAGAAGCAAGAACAGAAAGTGTTTTTATTAATTCAGAAAATTTTATTCCTTCTGATTTATTATCTTTTTCGAGTAGCAAATCAACAAAATTATTAATTAAATCCTTATTTTTTTCAATGTTGAAGTAGTCACTAAATATTGATTTTAAATAGTTTTTCAATTTATCAAATGTGTCAATTTTTTTATTATCTAAAATTTGAATTTTTTCATCAAAATTTTCAATAAGTTTAGTCACTAAATTGCTTTTAGTAATTGAATTGATTAATTTAATTAAATAATTGCTTATTTCTTTAATTTCTTTATCATTTAGTTCAGTATTTGCTTCTTTTTTTAATCAAATTGCGCCTAATTCAACTGCTTTATCAATAAATTTGGTGTTAGTTGCAATTTCTGATATCACCGTTTTTAATAAATCAATTGAACCATTCTTTTTAGAATTTTTTAAGAACGAAACCAAAAAATTATAGCCAGACGAGAAATCGTATTCATTATTTCCACTAATAAAATCATTTAAAATATTTTTTAAACAACGTAAAACTTTCTCGCTTCTTATTGTTGAAATTAGTTGATCTCAAACATTTTCAACAAATTTTTGAATTTCTTCTTCATTGTTGAATATCTTTTTGTAATTAATTGATTTAATGAAAGCAACGATAGGCTGAATAACAACATAATTTCCAATTTGATCAATCAATTGACTGAACAAACTATTATTCTTAAATAATTCATTTACTAGAAGCGAGATAATATCTTTAAATATTTCGCTACCAAATTCGTTTAAATTCTTCTTTGCATTTAATGCTATATTTTTATAAAATCTTAATATGCTTTCTCTTGAATCTTTATCAATTAATTTGCCTATGTTTTTTGGCATTAATGCATTATTTTTAATTGCATATTTTAGAGCTAAGTGTTTTAATCCTTGTTTGTAGTCGTTAATATTTTGTATTTTTGGTTCAAACGATAGGATTTTAAATAAAAGCGAGTTATCCCTATTTAGGTCGTATTTATCAAAAAGAATTCCATTTAGAATTTCTGAAATTAAATCCCTATTTTGGTGGTCATCATCTAGTTTAGAATTCATTAATATTTGAATAATAATGTTTGATATTTGAGATAAACCATCGAAGTTTGAAATGTTGTCAAAAAATGTTGAAACAAACAAAGAAATATTTGGTTTTTCAAGTAAATCTAAATCATATGATTTGTATAAAGAATCAACAATTTTTCCGGTCAAGTAGTTTGCAAGTTTGCTATCTTTTGAAATTTCTTTAATTATTAGGCTAACTAAATCATTTACATCTTTGCTTGAATCATGAACCAATGTAGTAATAAAGTCATCAATTTTTTCAAATTTAAAATATTTATCTGAATTATTAATTAACAAATTCAACACTTTTTGAAAAATGTTGTCTAAACCTTTTTCAATATCTTTGTTATTGAAAAGATTTTTGAATAAATCAACAGTAGTTTGAGGCAATAACCTTTTTAATATTTTTTTAATGAATACATCTGAAAATATAGCCTTAAATAATTTTGCATATTCGCTTCTTTTAAGATCTTGCCTTAAAGATTTGTTATAGAAAATTTCTTTTAAAATTTCATAAATATTTTGTTTAGAAGTAGTTGTTGAATAAACAATTTTTTTAATTTCTTCAAAGTTTAAACCTGCATATGATTTTTTATTAATAGCATTATTTAATGCATTGTTGATATCTTGAAACGATTTGCTTAAATTTTTTGAATCAATAAAAAGATTCAAAAAATTAATAATAGATTTTTTATTTTCTTTATTCTTTAGAAATTCTAATAAATATTTATTAAAGTTGCTTAAATCTTTCGGATCCAAGCCTATTAATCCGAATAGTATTGAAAATGTATTCAAAATTTCTTTATCTTCAATGTTTTTGTTTGAATTTAACATTGATTTTAATTGAAGGGTTATATGGTTTGAATTGTATGAAGAATTAATATTTTTGATATCTGCATTTTCTTCGAATGTATATTCTTTTTCAAAAGAATCTACATTGTTGCCATTTATTCCTAAAATCATTGAAAAAATTTCGGAAGGAGTTTTGCTAAATTCAAGTGATTGGCTAAATTCATTTAGATTAGAATTATTATCAACTAATTCTTTATATTTATTATTAGGCAATGACATTTTCATAAAAATATCTTGCGCTAATTTTTTGTATAAATTATTAGATGGGAAGCAGTCAAAGAAATTATTGTTTAGTTTATCGGCATTATCTTTGAGATTTTTAATGTCATTATTAATGTTTAAATAATTTAGCTTGTTTGTTTTGGCAATGTTTGCTAATGATTTGTTTAATTCTTCTATGCAATCATTAGTCAACGAAATATTGTATTGTTTGTCAATTAATTTTGTTAATTTCAAATAAGGCGATATATAACCCACGAGATTAATATTTGCCTTAGGATTATATTGTTTAATGATTGAAATTAATTTTGAATATGAATCTTTAAAATTTTTACAATTCTTTTTAAATTCAAGAAGAATTTTTGAAAAATATTTCAAAATTTCTTCTTTATTTTTGTTTTCTCTTAAAAGATCAAACGCTTCTTTAAAAAATGATGATTGTTCAAAAAGGTCGTTAAATCCCAAAGAAATTGTAATCAGGTTTGCTTGTTTTATTTTTTTAATTAGCTCTTGTAATTTTTTATTAGAAAAATCACCAAATAGATTATTCAATTTGGTACTATTAGATTTTGAAAGTAAATTTTGATTATAGGAAATAACTTGATTTAAATGAGCTGATTCCTTTTTGTCATCTAGTGAAAGTAATGAAATTCAATCATTAACATTTGAAAGCGGCAAACCGTAATTATAATAAGTTGATAATGTTGTTTGTTTATCAGATAATAGTTTTATAAAATTAGCAATGTATGAAGGATACGATGTTCCTAAAACTAAATCGTTTTTGCTATCATATTCATTGTTGAAATAGTAATTTGAGCTGCTATTATAACCTGCAGCAAAGCCGTCGCCTAATGCAACATAATTAATTTTTTTAGAAACTTTATTTTCACTTTGAGTTATTTTTGTATCCGGCTTTTTAGTTTTGTCTTTGCATGATATAGCAACTAATGAACTAGAAGCAATTGAAGATGTAATTCCTAAAGCAACAATCGCCTTGAGGATTTTTGTACTTTTTTTCATAAATACCTCCTAACCAATAGAAATATCTTCATAAATATATTGATATCTTTGTGGTTTTGATTTTGAAAAAGTAAAAATTTTTAGAAATTGTGATATTCCTAATTGTCCTACTATCATTGTTATAATTAAAAGTATTTTTCCAGTAATACCTAAATTTGGTGTTAATCCAATATTAAGTCCTGAGTTTCCGAATGCTGAGAAAACTTCAAACATTACATAGACCATTGGATATGACTCATTATAAACATTTTTTAAATCGCTTGGAAGTGTATGATATATTACTATTGTCATTATTAGAGTTATAAAAATTGCAATTGCAAAAATGTTAACTGCCTTAATTAAAGATTCTTTTCCTATTTGTCTATGAAAGGCATTTATGTTTTTGCGATTTTTAATTAAAGTAATTATACTCATAAAAATTATAGCCACTGTAATATTACGAATACCTCCAGCAGTTGAAACAGGAGAAAATCCAATAAACATTAAAATACCATGAACCATAATTGTAGGTTGAGTAAAATTATAATAATTTACAGTTGAAAAACCTGCTGATCTTGTTGACATAGTTTGAAAATATATTGCAAATACTTTGTCGCCTATCGAACCATATTCGCTTGAGTTTCAAAAATAATTTGCACCCTTAGCAGTTGTTTCAAAGATAAATGTTAATAATAAACCAATTAGTGACGTAAAAATGTATGTAATAATTGTAAATTTTGTAAATAATGTAAAGCAATGACGAGGATTGTCTTTCTTGATGCTTTTAATTTTTTGTCAAACATCATAGAATACAGGGAATCCCATTCCACCAATTAGAAATAAAATTGATGTTAAAAATAAAAAGTAATAATTATGATAATAAGGTTGCAAACTTTTTGTGCCAATTATATCTAATCCAGCATTATTTATTGCGCTTAGTGTATGAAATATTCCAAATCTTAGTGACATACCAAAGTTATGATAAGGATTTCTTATTGATTCTTCATAAGGATTAAATGTTCATGGATTATTTTTACTTAATGCATTTAATTGAGACATCTTTTCATCACTAAAAAGTGCTGTATTATCATAATAAAATATTAAAGAAAATATTAAAGAAAATAATATAGAAATAATAATTAAAAAACTTATTGAAACTTTTATCATTTTTTTTGTTTCATAAATATTAGAACCGCCTCTTTCAGTTTGGTGAATTTGGCCACTAAATAAATTCGTTCTTAATCCAAGCAATGATTGAAAAATATAAAGTTTAAATGTAAAAATTCCAATTCCGCCAAGAATAAAACAGACAGCTATTAATATCTGTCCAAACATGTTAAATACTTCACTAACGGCAACTCCACTTAAACCGGTTGTTGTAAATGATGAGGCTGCTAATAGCAAAGCGTCAAGATAACTAATATGCTTTGAAGGATTATGACTAACTTCTCAATATAACATCAAAGACATTAGAATCGTAAAAAGTATATAAATCAAAAAGATAAATCTTATTGTTCCAAGTTTCTTTAAAAACACAAATAAGACGTTTGATTTTTTTGGATTTCCCTTTTTAATCATAGAAAGAATTTTACCACAAAGTAAAAAAATGTTATTTTATAATACTTTACCATACCGCTTCTGTGTAAAAAAACTATAATATTTAAGCTAATATATTTAAAATTCAAAATTATTTTTTAAAATATTAAAATTTTGAAATGAAAAGAGGTTATTATGAGTTTATTTAAAAAGATACGTGAAATTTGTATTATTGGTATAGGTCGTTATGGTTCGTCGGTAGCAAGAACATTATTGGCCGATAGAAATAATAATATAAGGATAGTTTTAGTTGATGAAGATGAAAAACATTTGTTGCCTTTTAAAGATGAAGTAGAAATGATCTATGTTGCCGATTGTGCTGAACAAAAAACATTAGAAAGTCTTAATATAAAAGATTTTGATGCCGTTATTGTTGGCGCTTCAAATAATATTGAAATAGTTGCTGCTTTAACTGAATGTGGTGTAAAAACTATAATAGCAAGAGCTTCAACAAAAAGACATGCCCAAGTTTTAAAGCAAATTGGTGTTAGCTCAATTGTTTGTCCTGAAGAAGAAGCCGGAAAGAAAACTGCAATGTTAGTTGCAAACCCTGCGCTTGCATTATATTCAGAAAACATGGTTGAATTGCAAGACGGATTTGTTTCTGCTTCAATATATGTTAAAAACCCTGCTGTTGCAAACAAAAAAATTAGTGAATTAAATTTCAGAAATAAATACAATGTTTTAGTAACTATGATTAAGAGAGATAAACATTCATACATTCCTGAAGGTGATTTTGAAATAAAATTAGATGATTTAATAACTCTTATTGGCCAAATTGAAGATATTATTCAAGTTATTGAAATTTGTGCAATGAATGAAAAATAACTTAAAACAAGAACAAAAAAATAGCAAATTTTTTCTAGTTTGCTATTTTTAATTTTAATTTGTAAATATAGTAAAATTATAAATATTATCAATTTACAAAAGGAGATTAAATGGCTATTTTAGACAATGAGCTTCTTAAAAAATTAGAAGCAAAATACGAATCTAATTTAAATAACAAAATTATTGAAAATGCTATTTTTAAAAATGGTATTAAGGCTTCATCAACTAATAATCAAGTTGTAAGAAAACATGATTTTCAATTTTCTGTTGAAGTAAAACCTGGTTCAATAACTAACCAAAAACATTCAGGTAGATGTTGAATTTTTGCAGAACTAAATGCAGCAAGAGCAAAGGTTATGAAGGATTTAAACATTGAAGATTTAGAATTTTCACAAAATTTTATTCATTTTTATGACAAATTGGAAAAAGCAAATGCATATCTAACATGAATTGCAACAACTGGTTTGGAATTAGATAATGAAGATAGATTATTTAGAAGATATAATGAAAACCCTATTTCGGATGGTGGATATTTTGAATTCTTTTTAAATATTGCTAGCAAATATGGAATTGTTCCAAAATCTGCAATGCCAGAATCATTCCATAGTGAAGCAACAAACGATATGGTAGAACAAATTAATTGAAGACTTAAAGCATATACATTCAGAATGCGTGAAGCATATAAAAAGAATCATAATATGAGTGAAGTAGAAGCAATTCAACAACATGCTTTGGAAGACGTTTACAACATTTTAGTTAAGTCCCTAGGTATGCCTCCAAAAACATTTATTTTTGAATACTATGACAAAGATAAAAAATTTGTAAGATTGCCAGAAATGACACCAATAAAGTTTTTTGAAACATACATTAAACAATTTGTTGAAAATAAGGTTGATGTAATTAGCGACCCAAGAGAAATTTACCCTTACTATAGACAATTGGTTGCTCCTTCGGTTTTCAATTCGGTTGGTGGTATTGAATATAAAATGTTAAATATACCAATGAATGAATTAAAGGCAGCAGCGATTAAACAATTGCAATCTGGTCAATCAATTTGATTTGGTTGTGATGTTACAACTTTTTATTCAAAAGAAGGAATTTTTGACACTAAATTATATGACTATGATTTAACATTAACAGAAACTCCAAAAGAATTTAATAAGAAGGAAAAATTCGAATCTCGTGCTTCAGTTATTAGCCATGCCATGAACTTAATTGGTGTTAATTTAGATGAAAAAGGAAACCCAATTAATTGAAAAGTTGAAAATAGTTGAGGAACCGAAGTTGGTAAAAAAGGCTATTGATCAATGAGCGATTCTTGATTTGAAGAATATGTTTACATGCTAATTGTTGACAAGGAATTTTTATCAAAAGAAGCAATTGAAGGTCTTTCAAAAGAAGCTATAGAAATTTCTCCATACGACCCATTATGTGAAGAATAATAAGTTTAAAAAAAGTTGCACATTGCGACTTTTTTTATTAAAAATAATAATTTATATAATTATTAACTTAATACATTTATAATTAATACATTAATGTATTAGAAAGAGGAAAAATGTTAGGTGTGGACTTAACCGATATTAGACGATTTAAAGAATATAGTGAAACGCTTTTGATTAAGTTCTTACATCCGATTGAAATTGAACAATTTAAAAAGGTTTCGATAACAAAACGTGCTGATTTTCTTGCGACCAGATGAGCAATTAAAGAGGCAATCTTTAAAGCCGACAATCAATATTATTCATTCAACCAAATAAATATAACAAAAGATGAAGTTGGACGCTACGTTTTTAAAGATTTTGAAATTTCAACCTCTAATGAAGGAAGATACATAATTGCAGTAGTTTTTAAAAGATAGAACAAATAGTAGGAGTTATTTATGAATTTAAGAACAAGAATATTTTTTAGATTTTTTGGTTTGTTACATTGCATATCGTTTTTATGTGCAAAATCAAGACGCGATTTTAAAATGCCTGATTATTACAAAGTAGTAGAAAAAAACGACTTTTTGCAACAAAAAAGTAATGCCATTTTAAACCATTTTAATATTTCTGTTAATGTTGAAGGGTTTGAGAATGTCCCTAATGGACCATGTTTAATTATTCCAAACCATTCAACGTATTTAGATCCACTAATAATGTGATCAGCATTATGAAACCATAGAGATGGTTCAAAACAAAGTAAATTAGTAAATTTCGTTGCAAGAAAAGAAATAACAAAAAAGAAAATGGTTAAAAGAATAGCGGATTTAGGTCAAACTTATTATATTGATACTCAAAACCCTAGAGAAGCATTGGCAACCTTGAAAGATTTCGGTCAATTTATTAAAAGAAATAAATCATGCGGCGTTATTTTTGCTGAAGGAACAAGAACAAAAGATGGAAAATTAGGCGAATTTCATAGTGGTGCCTTTAAACTTGCTCAATCTTCATATTTAACAATTGTTCCAGCAACAATAAATAATGCAGCAAACGCATTGGATTGAAATAGAAAGGAAAAATTAGAAGTTAAGGTGACATTTCATAAGCCAATGAAGCCAATTCAATTTCAATCACTTGATAGCCGTGTATTAGCAGAACAAGTTAAATCGATAGTTGCATCAGATTATATTGATCAAACAATTACATCAAATGAAACAATCAAAAACAAATATTCAAAACGAGACAAAGTAAAAGAAGTTAAGAGATAAAATATGTCCGATTTAGAAAAACCAATTATTAGAGAAATTTCAATAAAAGAAGAACCAAACAAAGATCAAAACAATCAAACAAATTCATCAATTTTTAAAGAAAAACACGTATTTAAATTAAAAAATTTTGATGGTCCACTTGATTTGTTGGTTTCTTTAATTAAAGAGAAAAATATAAGCATTTTTGATGTTGACTTATCTGAATTAGCAAATCAATATCTTGAAATAATTCATAGTCTTCAATCTTATGAAGTTGATATAGCAAGTGAATATCTAGTTATGGCTGCACATTTATTACAATTAAAAGCTAGAATGGTATTGCAAGATCCTGAAGTTGAAGAAGAAATTAAACAAGAAAAAAAGAGATTGCTTGAACAGATTGCCGAATATGAAAAATTCAAAGAAATTTCTTTACTTTTACGTGAACAAGAAGAAAAAAGATCTAGATTGTTTTCGAAAGAACCAGAAGACACACAAGATTTCGTAAGAGATATTGATGGTTCGATTCTTGATGGACATTCCAATAGTTCAAAATTAGTTGTAACTTTAAGAAAAATGTTTGAAAGAACATATGCTGAACTTATTAGAAATATTACTATTTCAACAATAGCAGTGAGTCCCGAAGAACAAAAAGAAAGAATTATTAAATTATTTAAAACAAAGTTGCAAGTAACCTTTAAAGATGTTTTTTCTGTTCCAAGTTTGGGACACTTTGTTATTACTTTATTGGCGGCATTGGATTTAGTAAGACAACAAATTATTGAAATTTATCAAGAAGAAAATGAAGGCGAAATTCATTTTAAGAAAGGGGCTTTATATGAAGAATAAAATTATTGAAGCTTTGCTATTTATTCAAGGCTCTGAAGGGTTAAGCAGTGAACAAGCACAACAAGTTTTCAAATTGAATACAATTCAAGAAGCAAGACGATTATTAAGGGATTTTAAAACATGCTTTAATAACTTGGAAAGAGGAATAAAAGTATTGGAATTCAATGATGTATTCAAGTTTTTAACTATTGAAAGCGTCAAAGATTATATTAGTGATTTAGTAACTATTACTAGAAAACAAAGATTATCAAATGCCGCAATTGAAGTTGCGGGTATTGTTGCTTATAAACAACCTATAACAAGAGGCATGATTTCAAACATAAGAGGTGTTGTAAGCGATGGAATTGTAAGTAGTTTATTAGAAAAAGGAATTATTGAAGAAGTTGGAGTAGCTCAAACTCCAGGCAACCCTATTTTATATGGAATTACTAACAAGTTCTATGATTATTTCAAAATAAAGACCTTAGGAGAATTGCCTCCTTTTCCTGAATTTAATAAATATAGCAGTGAAGAAGAGGAAGAAATTGATAAAGACTTCAATTTGTTTGATTCTCAAAGACCAGAAACGGACAATGATGGAGTTTTAATTTCTAATGAGGGGGCAGAATATGATAAATTTTAAGGCTAAAAAGGATGACGAAGGTCGTATCCTTTTTAAATATTTGGTAAAGGTTTGCGATAATGTTCCAATATCTAGAATAGAGCGACTTTTTAGAGAAAAGGATATCAAGGTAAATAATATACGTGTAAATGATAAGCAATACAAAGTCAAAGAAGGAGATTTAATTTCAATTTATGGATTAATTGAAACATCGAAACATATTGACAACAAAGCTAATACAAACATAAATTTTAAAGTTATTTACGAAGATAAAAATCTTTTAATTATAGACAAGGCGCCAAATATTGCAATGCATTCTGAAGAAAATTGTTTAGATGATCAAGTTTTAAAATATTTAAATTTTAAAAAAGTTTCTTCGTTTAGACCTGCTTCAATTGGCAGAATAGACAAAAAAACTAGCGGTTTGGTTGTTTATGCAAAAAACTACAAAACTCTAGTTGAATTTGAAAATAAACAAAATAAATTTGACAAAATTTACCAATTTGTAAGTGATTTTGATGAAGATGTTTTGGATATAACAGTAAGACTAAAAAAAGATATTAAAAACGAAAAAATGATTGTCGATAATAATTTTGGAGTTAAAGCACAGACAATATTTTATAAAGAAGGAAATAGAAAATTTGCCAAGATAATAACAGGCAAAAAACATCAAATTAGGGCGACATTAGCATATTTAAAAACACCTATTTTAGGAGATATTAAATACGGCGGAAAATTTGCTAAAAGAATGTATTTGCATTCATATTCAATAAAATTTCATGATCTTTCTGATGATTTTAAAGAATATAATGAACAAGTATTCATTTGCTATCCAAAATGATAAAAACGATTAAGGAGTTAACATGGAAAAAGAAGAGATTAAAAAATTGGCTAATGATCTTTTATTTGAACCAAGTGAAGAAGTTATTAAATTAACTAATAACTTGCTTAATTCAATAGAAAAAGGTTTAAAAGATTTAGAGGAATTTGACTTACAAGACATTAAACCGATGTCGCACATCAATGAATCACCCATTTGTTTTGAACAACTAAGAAGCGATGAACCAGATAAAAGTTTTTATTTGAATAAACAAGATTTATTAAATAATGCATCTGAACATGATGAAAATTATGTAATTATGAAGAAGGTAATAAATGATAAATAAAGGTAATTTAAAAGAAGCAATTTTACAATTAAATGCTGATAAAAATAATGCGGTATCATTTGTTTTTAACGATGCTCAATTAAAAGAACAAGGACCGCTTGCAAATTGTGTTTTTACAATTAAGGACAATTATGCAGATTTGAATGTTATTGAAAGCGCTTCAAGCAGATTTCTTTCTAATTTTAGACCTTCATATCAATCTACAATCTTAAAATTATTAAATGAAGCAGGAGCAACGTGCGTTGCAAGAACGAATTTAGATGAATTTGGTATGGGTGGCAGTGGAGAATATTCATGCAATGGATTAATAAAAAATCCTTTGAATAGTAATTATTTAGTTGGAGGTTCTTCATCAGGTGCCGCTGCAACTTTTTCTAAAAATATCTCGTTTGCAATTGGATCTGACACAGGCGATTCAGTTAGAAAACCTGCATCAAATATTGGTCAGGTTGGATTTAAGCCAAGTTATGGCGCCATAAGCCGTTATGGATTGTCTGCTTTTGCTTCGTCATTAGATACAGTTGCATATTTTACACATAATGTTCAAGATGCAATAACTCTCTCATCAGTATTATTTAAGCAAGATTTAAAACATGATTTGACATCAGTTGATTTGTCGTTTAATGCAGATAGTGTCATTGAAACAAAACCACATAAAATTGCTTATTTGGATTGTTTTGACAAATTGGATATATCTGTTGCTGATGCATATAAAAAATTATTGAATAAGCTAGAAAAAATGAATATTGAACTTGTAAAAATTTCAATAAATGAAAAGTTACTGCAAGCCATCGATACTGTTTATAAGGTTATTGCTTTTTCAGAATCTTCATCTAATTTAGCAAATCTTGTAGGTGTTGAATTTGGCAATAGAATTACCGGCAATTCTTGAGAAGAAACATTTAATTTATCAAGGACAGAAGGTATTGGAAAAATGGTTCAATCAAGAATGATTTTAGGATCTTATTTTTTAGAAGATAATAATCAAATAAAGTATTTTGTTAAGGCTAAAAAAATGAGAAGGTATTTAAGTGATTATTTCACTAATGTTCATAAAAGCGCAGATATATTTATCTATCCAGCATCTAACATGTCCGCTCCTCTTATTGGAAAATCATATGAACCAAGTTATATGGATTATATTTTAACAAATTCTAATTTGACAGGAAATCCTTCTTTGACATTAAAATTAGGAATTGACAAAAATAATAATCTTCCATTTAATTTAGCAATTGACGGAGAAATATATAAAGATTGCAAGATGCTAAGCCACGCATTATTTATTGAAAGAGTATTGGAAGGTGAAAAAAATGAATAATGATTGAGAAATAGTTATAGGAATTGAAATTCATATTGAGTTAAATACCAAGACAAAAATGTTTTCTCCAGTTCAAAATGAATACGGAGATGAAGCCAATACAAATGTTTCAAATATTGACCTTGCCTATCCTGGAAGTTTGCCTCTTTTAAATTCTAGTGCCATAATTAAGGCAATTAAATTAGCTAAGGCGCTAGAAATGGAAATAGACCCATTAGTTAGATTTGACAGGAAAAATTATTTTTATCCCGATTTAACAAAAGGATATCAAATTACTCAACAATTTCATCCAATTGGTAAAAATGGTTTGATAAGAGCAAAGGTTCAAAATTCATGAAAAACTTTTTCTATTGAAAGAATTCATATGGAAGAAGATACTGCTAAATCAATTCACGAAAATGGTTTGACATATTTAAACTATAATAGATGTGGAGTTCCTTTAATTGAAATAGTTTCTAATCCTGTAATGCACAGCGCAGAGGATGCAATGGCTTACGTTGAAGCTATAAGACAAACAGCATTAGTTTTAGACATTAGTGATGCAAAATTAAATGAAGGAAGTTTAAGAACTGACGTTAATTTATCAGTAAGAAGAAAAGGAGAAAACATCCTTAATACTCGTACGGAAATAAAAAATTTAAATTCTCTTTCAAATATTAAAAAAGCAATTGAATATGAATCTAATTTCCAAATTCAAATGTATGAAAACAATTTAACTTTTAATCAAGAAACAAAGCGTTTTGATGAAAAAATGCAAAAGACTGTTTCAATGCGTTCAAAAGGTGATGCAATTAGTTACAAATATTTTCCAGATCCAAATTTACCTTATATTCAATTGCAACAAAAATTTATAGATGATATTAAAATAGAAGAAATACCATATCAAAAAGAACTAAGATATCTAAAAAATGGCTTAAATCAAGTTCAAATATCTCAATTGTTAAATAATATTTCTTATGCAAAATATATTGATAGTATAAACTCAAAAGATTTTAAAAAAGCCACAAATGTTTTCTTTTCTGACATAGTTACCTATTTAAATAGTAACAATAAGAATATTGAAAATTTATCATTTAAAGCAGATGATGCGAGAAAGGTTGTCGAATATTTAATTGATAATAGGATTAATAAAAATAATATTGCTGAAATTATCAAAATTAAAGATCAAAATCCAAATTTAAATATTGAATCTATTATTAAAGACAATGGCTTTTTAATTGAAATAAAAGAAATTGATTTGAACGAACTAGTTCAAGAAATATTAAATGAAAATCCAAACTTGGAAACTGAATTTAAAAACAATTATAACCGTGCCCAAAAATTTATGCAAGGTCAAATAATGAAAAAAACAATGGGAAAGGCAAATCTAAAAAATCTTGATGAAATAATCAAAAATAGGTTCAATAATGAGTAAAAAATGAAATCCAAATAAGCTACCTACGGGTTGAAAAGTTGATGAAGATCAAGCAAATAGAAAACCAATAACTATTTCTTCAACCCAAATTATTGCAAGAAAAAAACGAAACAATATTTTAGAAAAAATTCTTAAGTGAATTATTTACGGAATTATTTTTATAGCAATACCTAAATTTATTAAAAGTTCAAAAACAAAAGGAAAAGAAATTGTAAATTCTGCATATGAAAAAATAAATTCAAATGAATTTTCTTTTGTCCCTGCTGGATATGCAATGTATCTATTTTTGTCTTTTATTCCAATCTCAATATTGATGCTTGGAATTATTGGATCGGTTGATGAAAAGTATGAAGTGGTGCTAAAGAATGTAATTTTGGGACAAATTATTCCCGGAATTTCAGAAGTAATTCCATCATTTAGTACGTTATGAGGTTCTACAGGCAATGCAATTGCTTTAATCTTATTTGCGCTATCAGTATTAATTTTGGCATCGAAGGGATATTCAAAATTTATTTTTTCAATAGATGCTTTATATGAACACAAATCTCCATTCAGAGTGTGAAAATCGAGAATTAAAGGATTCATAGTAAGTGTCTTTGTCTGCTTGATATTGTCTTTAATTTTACTAGGGCTAACAGCCTTTATGGTATTTTTAATTGATAAAGTAGGACTTGGAAAATTTGATGCCGATATTAAAAAAATGAAATTAGCAGATTTTAATTTAACTACAGAATTTTATTTAATATATTGATTTACAACAATTTTGTTTTTACCTGTATTTACTTATTTAGGATTTCTATTTTATTTTGTATATGCCCCAAATTTTAAATTGAAAATGTCGCAAGCACATCCCGGGGCTTTGATAGCAGCAATACCAACAGCAATATTTATTTTGATTTTTGGTTCGTTAACATCCTTAATTCCTTATAAAAAATTTGGGATAGTTGCTTCATTTATGTATATTATTTTATTATTGAGTTTTACTTCTTATTTTATTTACATGGGAATTATTGTTAATTCAAGTTTTTATAAAACTTTTATTAATTTACCAACTGTAGAAAAACATTCATTTTGAAAAAAACAAAAAATTTCAATTTAAAGTCATTTGATTTTTTCACTAATTTTTCATTATTTATAGTTATTAACGACATTTAAAATATAATTATTATAATAATTAATAAAGCAAAGGAAATATATGAATAAATTACCTGGAGGGTTAAGACACTATATTTATTGAGCTCGGTCGCAGACAAATATTTCATATCGTAAATGAAACTCAAAACGTATTGCGTTTGTGGGTGTTTTGATTGCTATCTCAGTTGTCTTTTTTCTAATAAGTGTTAGAATCATGCCCATAAGTGCCTTACCAAGTATAAAATTTAGCTTCATTGGTTTGCCAATAAAAATAACTGGTTTTATTTTTGGACCAATTGTAGGTATGATAACTGGGATATTGGCGGATATAATTTCATTTGCTCTAGTTCCAACTTACTATAATGTTTTGTATACAATAGCGGTTGCGGTATCAGGATTTGTTCCCGGGTTTGCTGCGTACTATTTTTTCAATATTAATGAATTGTTTTTTTCAAGAACGTATAGAATTTTTAAATACAAAGAAACAATTGATTATTTTAAAGAACAATATGATATTGCACTTACAATAGGTAACTCTGAAGATATGCAATATTTTTCAGAAAAAATTGCTTTTTATGAAGTCAAGATCATAATTTTAGAATGTAAGAAAAAGCCAATTGCCATGATTAATTTTTCATTTATTTCAACATTGATAATATTGGGTATTCAAGTGTTGATAATTCTTGCAATTTTTTCTAACCTAGATCCTTCAATATTTGAAAAAAATAGATTTGTTAAAAACAAAAAATTCTATATGTTCTTAACTTCTATAGGATTTATAGGAATGATGATTTTTGTTTCTCTATATAGAATATTTTTAAAGAAAAAATTTGAAACCTTTTTAGAAATAATGGCAATAATTTCATTTAGCGCTGTTTTAGAAATAGTAAATGTTATCTTATTGGCGTGAGCAGATACTTCTACATTAAAGACAGATTTCTGGGTTAATTTTACTGCTCAAAGTCTCTTTAGCCCCGTTAAAATATTTTTTAATTTAGCAATAATATTAGCAACGTATAAAGTTGTTGCACCTTTAATTAAATCAAAAGAAGGTGGACATTTATAATGAAAAAAATAGCAATAAGTTCTTTAAAAGTTACCAATTTAAAAAGTATTAATGTTGTAAATGAACAATTTAAAAACTTAATTAATAATCTATCTTTTACACTTAATGCAAACAATATATTGGGTTTTGTAGGTGGAGATAGTGAAACAAATAATTTAATATTTAATTATTTAACTAATAATACAATAAATCCTAAAAGATATCAAGGCTACATTGAATTTCAAACAAACTTAGGTGAATATTCTTCAATATATAAGAACTCAAATTATCAAAATAACATAGCTTTTGGTTTTTATAATGATATTTTAAACAATGATTTAAATGAAGATAATAGCTATAGAAATATAAAAAAATATATTAACCACGGAGCAGTTATAGGGGCACTAACGGAAGAGTTTGTTTCTCAATGAAAGCCAGTTTTTCAAGACTATAAATTATTGCTTCAAAGTGAATTTGCCAAATTGCAATATGGTGTCGAAATTGAACTCGGAAGTAAATTAGACGACTTATTCAGTGAATTAAATCAATTTGATTTGCGAAATATCGACGCTAAAAATTTAAAATTTGATACTCTTTTTAAGAAATTTAAAGATATAGAAATTTTATATAGAACCCATGAAATGAAACTATTCGCATTTGTTAATGCAACTATAGCAAAATATAATAAGGGGTTATCGTTTTTAAAACGCAAGGAATATTTAGACAGTAAAAAGGAATACGAAGAGGTAATAAAAAAAATTTATAAGCCCTTTTCAAAGCCTAATAGATTTTTAAATCGAGTAAGATTTTTTTCTAAACTTTTAGAAACAAAAAAATCTTCAAAATCATCTATTAAAAAATTTGTAAATAAATTAAGGCGTGATTTAAATATTGAAAACTATTATTACAAATATGAATTAAAGAAAACAATAAAACAATCTTCGTTTATTCACTTTTATAAAAATCTATATTTTAATGGTCTATTATTGAAATTTATAAATAAAAATTTAAAGGCCATATTAGGGCTAAAAGAAGCAAAATCGTTTGAATTATTGTATGAAATTTTTGATTTAAAAAACAATATTACAAATGAAGTTAATGTAATTTCAAACATTGATAATCAAAAAGAATTAAAGAATGAAATAAAGGCTGTAATAAAAAATAATTTTTTAAATGATGTGATGCCATATATTGAAAGAATTAAGGCGTCTAGTTATGAACACACTCAAGAAGTTATTAATGATGTTACAAGAGAAATTCAAACGTCTAATAACTACGATTTAAACATAGGCGAGTTTGGCCTTGCAAACAAAGCGTTTGAAAAATTAAAAACGGTTGAATCAAAATATAAAGATGCAAAAAATGAATATTTTTGAAATTATAAAACTGAAGGGAATGCCATTTTAAAGGAAACAAAAAAAGTTTTAAGAAATTTTAATAAATATTCAAAAATAAACTCACATAAATTAATGAAATTAAAACTAAAAATTTTTAATTTGATTAATTTAATAAAAATTAAGGAATTTAATAATTCAGTTCTAAATGAGCAAGTAAAAAAAATAAAAGAAAATCTACAATTTTTAAAAATTTTAAGAAATGAATTTGATTCTTATAAATATGTTGTAAGCGATTTTAAAACGGCATCAAAAAAACAAATAAAACTTTTTGTTTCAAAAATTAATATTTACAAAATTATTGCGAAGGCAAATATTGACGTTAATTTTTTAGTAGAGAAATTAGGCTCTTTAAGCGATGATGAAAAAATTAGATTAGAGTGCGAAAAAATTTTAATTAATAATCCTTCACTTATAATAATAGGACCCAATATTTCTAAATTACCATATGAAAAGCAATGCGAAACATTGAATCAATTGAATAAATTCGTTTTGGACAATCACAAAATGGCAATTTATTTCTTAGACAATATAAATATTGCTCAAAAAATAACTACAGATTTATATATTATAAATTCATCAAAAATTATTGAGCAAGGAAAAACTAGTCAATTAATAAGGAACCCAATAAATCCTATAGTCAAAAATATGTTAAATTATAAAGACGAGAAGACTCAAGAGGATTTAAGCTTTTATATGTCTCAAGAAGACAATATGGAAAACATATACAAATATGAAATAGAGCCATACCACTTTGTTTGATGCACATGAAGTGAATTAAACAGGTGAATAAACGCCAATAATGTTAAAAACAAAGAGCTAATGAGTTTATTTATTCCGCAAAATACTTTTCAAAGTAAAGGAAATAAAACAATAGACAATAAGGAACCATTCGAAGAAACAACGCTTATAAATTTAATCGATGACGAAGATAACGAACTAAGGAATTTGAACATGGAAAAAACTTTTAATCATAAAATAGTTGAAAAAGACAGATGCAAAAAATGAAATGATATGAAGATTTTTCAAACTCACGATGAATCTAAAAAACCATTCACCATTATTTTGCCACCCCCAAATGTAACCGGCAATCTCCATATTGGCCATGCCCTAGACAATTATATTTCTGATACAATAATAAGGTTTAAGAAGTTACAGGGATATGATGTATTGTGACTACCAGGAACAGATCATGCAGGTATAGCAACTCAAGCAGTAGTTGAAAAGAAACTAGCTTCTCAAAATCTCAATAAATATCAATTAGGAAGAGAAGAATTTATTAAAAAGGTTTGAGAATGAGTAGATGAATATTCAAAAAATATTCATAACCAATGACAAAAATTAGGTTTGGCCCTTGATTATGGTTTTGAAAGATTCACTTTAGATGATAATTCTAATGAAGCCGTATTAAAGGTATTTATTGAACTTTATGAAAATGGTTTAATATATCGTGACAAAAAAGCAATAAATTGAGATCCTGTATTAGAAACAGCTCTTTCTAATATAGAAGTAGTACCAAAAGAAACAAAGCAAAAGATGTACTACATAAAATATCCAATTAAGAATTCAAAAAACTTTCTTGAAGTAGCCACAACGAGACCCGAAACAATGTTCTCAGATGTAGCTTTAGCAATAAATCCGCAAGATAGTAGAGCAAAACAATTAATAAAAGAAATAATAATACATCCATTAACAAATAAAGAAATTCCAATTATTTTATCAAACAAAATAGATCCTAATTTTGGTAGTGGAATAATGAAAGTTTCAGCCCATGCTGTTGATGATATTGAAATTATTATTGAAAATAAACTTCCAATAAATGAATGTATCAATAAAGATGGGAAATTAAATGATTTAGCAGGTGAATTTAAGGGATTAGATCGCTTTGAAGCTAGAGAAAAAATTGCACACTTATTAAGTCAAAAAGGACTTTTAATAAAAGAAGAAGAAGTAGTTTCTAATATTGGGTATAGTGAAAGATCAAAGGCCCCAATTGAAATTTTAGTTCAGCCACAATGATTTGTTAAGATGTCAGAATTTTCAAAGATGCTTTTAAAAGATTTGCGCAGTGACGAAGGAGTAAAAATTTTTCCACCACGTTTTGAAAGTACACTAGAAAATTGAATGGAAAATGCTCACGATTGAACAATTTCAAGACAAATTTGGTGAGGGCATCGGATTCCCGCTTGGTACAATGAACAAAATGAAATTAAAGTTCAAATTCAAAAGCCCGAAGGAAATTGAAAACAAGACGATGATGTTTTAGATACCTGATTTAGTTCGGGACTTGCACCATTTGTGTTTTTAGGATGGCCACAAGACAAGAGCAAATTAAAACGTTATTTTCCTACAAGCTTATTAGTAACTGGATATGATATTATTTTCTTTTGAGTAGCAAGAATGTATTTCTTTAGTTTATATTTTATGGATCAAAAACCTTTTGAACATCTTTTATTGCATGGATTAGTTAGAGATTCGCAAGGAAGAAAAATGTCTAAATCGCTTGGTAATGGAATTAATCCAATTCAAGTAATTGATGAATACGGTTCTGATGTTTTAAGAATGTCTTTAATTTTTAATTGCACACCTGGCCTTGATATTAATTACAGTGATGAAAAAATCCAAGGCGCTAAACTATTTGCTAATAAATTCTGAAATATAGCTAGATTTGTTGAAAATATTCCTGTTTCGATAACTGAAAAGATTGATTTTAATAAGTTGGATGAATATGATAATTGAATTTTGAATGAATATTTAACATTCAAAAAAAATATTGAAGATGCCATGGATAAATATGAGTTAACTATTATTTATAAGCATATTCAAGATTTTGTTGTTGACAAATTCTCAAATTGATATTTAGAATTTTTAAAATTTAAAGAAAATTCATATTTAATACATTATTTATTCAAACAAATATTGATTTTGTTGCATCCATTTATGCCGTTTCTAACAGATTATGTTTTTGAAAAATTATATCATGAAGAATTGCTATGCAGCAATCTTCAAACTTTTGCTTTAAATAAAGAAAGTTCAAACAATACTCCTAAAATTATTTCTTTAATTACTGCTTTAAGAAAATACAGGGAAGATAAAAAGATTTCAAAATCTATTGAATTATTCTTCTATTTGAATAATCTTGTATTCAGCAGTATAGATTTAAAAATAATTAACAAATTAAGCAATTTTTCTTGAAAAGAAAATCACGATTTTTCACTAAAAACAGAAAGTTTTGAAGTATTTATAATTCAAAGTAAGCAAGACAAAGAAAATGAAATTCTAGAATTAAATAAATTAATTGAATTTAGTCAAAAAGAAATTGAATTTAATGAAAAATTAATTAATAATCCTAAATTTATGGAAAAGGCTAATAAAAATACAATCAATGAAAAATTAGCAAATTTAAAATTGCATAAAGAAAAATTAGAGTTTTATAAAAACCAACTCAAAGAAAAACAAGAAAAAATAAATTAATTAAAAACAAGATAAGAGTTTTTTAATAAACAATTATCTTGTTTTATTTTTTACTGTTATAAAATTGCATTAAAAATTATTATTTGTCTTGAATTGCTTCTATTCCAGGTAATTTCTTTCCTTGGACAAATTCTATTGATGCTCCACCGCCAGTTGAAATAAAACTAAATTTATCTTGATAACCTAATTTGTTTATTGCTGTAACAGAGTCGCCTCCGCCAACTACTGAATAGCAATCTCTATTTTGAGCAATAGCGGTTGCAATTCCTTTTGTTCCGGCTTCAAAGTTACTGAATTCAGTTACTCCAGCTGGGCCATTTCAAAAGATAGTTTTAGCATTTTTAATAATACTTGCAAATAATTTAATACTTTCTGGTCCAATATCTAATCCCATATTTCCATCAGGAATATCGGGATTTTCTGGACTTGTAAATTTAGGAGTTTTGTCAGCAAATTCATCGACATAAGCATTGTCAATTGGCAAAACAATTTTATCTTTATATTTGTTTAAATATTCTTTTGCCATTTCAACTTTATCTTCTTCAAAAATAGAAGTTCCAATTTCTCTATTTAAGGCTTTTTGGAATGTATATGCCATACCACCAACAATTAAAACCTTATCTGCTAGTTCAAATAACTTTTCTAGAACTTTGATTTTATCGCTGACTTTTGCTCCACCAATTATTGCAACAAAAGGACGTTTAAATCCTACTAATAATTTTTGAAGGTGTTCAACTTCATTATTCATTAAAAATCCTAATGCTGATTCTTTAGAATATTGTGAAATTCCATATGTTGATGCATGAGCTCTGTGTGCAGTTCCAAAAGCATCATTAACATACACATCGCCTAGACTTGCTCAATATTTTGCAAGATCTTCATCATTATTAGACTCTGCTTTATTATTTAAATCTTGGAAACGAGTATTTTCTAAAACCAAAATTTCTCCAGGTTTTAAGTTTTTAGCAGCATTTGTAACTGCTTCGCTTCTATTTTCGTTAATAAATTTAACGTCTCTATTTAATAATTGCGCCAATTTTTTAGCTACAATTTCTAAAGATTTAGTTTTCTTGTCTTCTTCTGTTTTAACTCTTCCTAAATGAGATAGAATGATTAAAGAAGCATTTTTACTCAATATGTATTTGATTGTTGGCAATTCTTCATCAATTCTTTTTGTGTCCATAATAGTATTGTTTGAAATAGGAACGTTAAAATCAACTCTTAAAATAACCTTTTTGTTTTCTAATTGAAGGTCTTCAATGGTTTTCTTCATTTTATCTCCTTGTGTGTCTTGTATGTTTTATATAAATAAAAATAATATTTTGTTTAAATTTATATAATTATAAAGAAAAAAAGTTATTTTAAAATCAACTTGGGAGATTAAATGGCAAAAATTTTAAATGCTCAAAAGCAAAAGGACACAAGAACTCTTAGCTATGATCCTGAAAAAGACAGCTTGACTTTGCTAATTTCAGAGTTTCAACTTTACAATAAAAACACCAAATTTATTAATGATTATTCAGAAATGGAAAAATTTCATTATTTTAAAGAATCTAGATGAATGAAAACAATGGATGAAAAATTACAAGATCAAGTGCAAGAATTTTCAAAAAGGCAAAAAAAATTAAATGCCAAATATAAAAGTTACATTGAATATTCTAAATGGTCTGAATCGCCATATGCAACAAAAACTAAACCGCTTACAAAATGAAATAGAATCATTATTATTTCGGTTTTCATTATTTTAGTAATTATCATGTTGCTAATCGTAATTGGCTTGAATAAATTGTGATAAAAACGGAGGTATTGACATGGAAATAAAACTATTAATTGTTTTACTAGTGTTGCTAGCAGGTGTTATTATTTTCATAATTGCTAATTATTCAATTACTTGAATTAAAAACAAAAAAAATTCAAAAAGTAACAAAAAGGTTTTGGGTGAATCTACAAAAATAAGAATTATTTATCAATTAAAAGAAGCCCTTGAATTTTTATCAAAGACAAAGACTGGTGCAATTATTACTATTGAAAACAAAGATAATCTTGATTTGCTAAGAACTGATGGTGTAATATTAAATGCTAACATTTCTGCTTCTTTAATTATTTCTATTTTTAATAAACACGCTCCACTTCACGATGGCGCAATAATTATTAGAAATAACAAAATTTATTATGCTGCCACATATTATAAAATAACAAAAAAATCAATTGATAATAAATATGGTGCAAGACACCGTGCTGCTATGGGTATTTCAGAAATTTGCGATGCTACAACTATAGTTGTTAGTGAAGAAAATGGCGGAATTACAATAGCTAAAAACGGAACTTTTAGCCCTGTTTTACTAGAAAATCTACAAGAAAGATTAGTCAATATTTTTAAGGATAATAATTAAAAAAATAAAAATTTTGAAATTATTAAAAAAGTATGGTAAAATGTATTTCGCAATTTGAAAAAAGCGATCGTTTAATTGCTCAAATTGCCGCTTAAAAAAATAAATATTATAGTTATATAATATACTCTCGCTCTTTTCTTATATTTATAATAAACCCTTATTTATTTTATACACATATACATACAACCCTTATTTTTTATATTTCCTTAACTTAGAACTTGAAGTTCAACTTATTATTAAATTAAATAATCAAAAGCTTATATTGTTCGATTTTTAGCGAGAGTAATCTTATTTTAAAAAATATTTCTTTCAAAATAGTAAACAAAAATGTTTGCTATTTTTTAATATTTTTGTTTAAAATATATAAGTACATAAATATCACTATTTTATGGCCAAAATTAAATCATTTACATTAAAAAAATATTTTTTTTGTTAAAATTACATTGCTATAAATGCGCGCTTTTGGACAGGACATAGTTGAAAGGCTATGCTAAGGTAGGTTAAACGCGTTTAATCAAAAGATTTAATAAATATTAAAAAAATTAAAATGTAATTTATTGAAAGGAAATTATTATGGCAAAATTAGATTTTGACCGTTCAAAACCACACGTTAATATTGGTACTATCGGTCACGTTGACCACGGTAAAACTACATTAACTGCTGCTATTGCAACAGTATTAGCTAAAAAAGGTCTAGCAGAAGCTAGAGACTATGCTTCTATTGACAATGCACCAGAAGAAAAAGCACGTGGTATTACAATTAACACATCACACATTGAATATCAAACAGAAAAACGTCACTACGCACACGTTGACTGTCCAGGACACGCTGACTATGTTAAAAACATGATTACCGGTGCTGCCCAAATGGATGGAGCAATTTTAGTTGTTGCTGCTACTGATGGTCCTATGCCTCAAACTCGTGAACACATTTTACTTGCAAGACAAGTTGGTGTTCCAAAGATAGTTGTATTTTTAAACAAAATTGATATGTTTAAAGATGATGAAAGAGAAGAAATGGTTGGACTAGTAGAAATGGACGTAAGAAGCCTTCTATCAGAATATGGTTTTGATGGCGACAATGCTCCTATTATTGCTGGTTCAGCTCTTAAAGCATTACAAGGAGATCCAGAATACGAAAAAGGTATTTTGGAATTAATGGATGCTGTTGATACATACATCGAAGAACCAAAACGTGAAACAGACAAACCATTCTTAATGGCTGTAGAAGACGTATTTACAATCACTGGTCGTGGAACTGTTGCAACTGGTAGAGTTGAACGTGGTGTTTTACAATTAAACGAAGAAGTTGAAATTGTTGGATTGAAACCAACCAAGAAAACAGTTGTTACTGGTATTGAAATGTTCCGTAAGAACTTAAAAGAAGCACAAGCTGGAGATAACGCTGGATTATTGCTACGTGGAATTGACAGAAGCGAAGTTGAACGTGGACAAGTTTTAGCAAAACCTAAGACAATCGTTCCTCACATACAATTCGAAGCAACTGTTTATGTTCTTAAAAAAGAAGAAGGTGGACGTCACACACCATTCTTCCACAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACTGGTGGAATTGAATTCAAACCTGGACGTGAAATGGTTGTTCCTGGCGACAACGTAGAATTAACTGTTACTTTAATTGCTCCTATTGCTATTGAAGAAGGAACCAAGTTCTCTATTCGTGAAGGTGGAAGAACTGTTGGTGCCGGATCAGTTACAAAAATTCTTAAATAATTATCAAAATTACTAAAAGAAATTTTAAATTACAAAATTGGATTTGTATAAATCAAGTCCAATTTTTTTTATATTTTATTTAGTATGTTTTGATTGCAAATATTGCCGCTTTTATCTTCTATTATTAATTGTTAATAAAAAAAGTAACCATTTGTTCTTTAAAAAATGCCCCCCCCCTATTTTTTAATTATTTTATTAACTTTTTAGTTAATAAAAATAAAATTGATTAATTAAAAATATAACTAAATTTCCATTTTTAATATAGGAGAACGAATGAATGAAAAAAAGAAAAAAATTGCCATTCCATTGGCAATTTTGTGTGGCGGTTTAGCAATTGCTACAACAGCGCTTATTGCAATAAAGGCTCGCCGTCATAAAATTGCAAATCAATCGCAAAAAGAAAATTTATTGCAAAATTTTAAAAAATTACAAAAGCAATTAAATGAATTGCTAGGATATAAAATTGTTAATGAAATAAATGTTTTTCATGAACAAGAAGTTTTGCAAGGATCACTAAAAATTAATAATAAATCTGAAACCAAAGTAATTGAAGAAGAAACTTTAAGGTTAAAAGATGCTATTACTTTATTAATATCAAAAATAAAAAATCAAATAAATCAAAAAGAATTGGAATTTGCTAAATTTAATGAAATAAAAGATAAATTGCAGGAATATATCAAAAACGAGTTATCGAAACAAGAATACGAACATATTAAACAAAATATCGAAAATGAATTAAATAAATATACTCCAATTAGTCTCGAATCAACTTTAATCGAAATACAAAATGCAACAAATAATCTAATTAAATTATTAAATGAATCAACCAAAGAAAAAGATAACATTGATAATTTAAATGCCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAATTAAAAATGCAAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAATAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAATAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAAGCTAATCAAGCAGTTGCTTTAAACAATACTGCTTCAATGCAATCAGCTAAGACTACATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTGCATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTACATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCAAACAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCATTACAAGAAGCAAATGCTTCTAAGAGCCAAGCAGATAATTCTAATAAATTAATTAAAGAACAATTAAATGCTTCAATTACAAGTGCTAACCAATTATCAGCAAAATTAACTGATAATGATAACAATATTCAACAAGCAAAAGCTCAATTAGCCCAAGAAATTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTACATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAATAAATTCAATGAATTAAAACAAACAAGAAACCAAATTGATGAATTTATAAAAACAAATAAAAGCAATTCAAATTATTCAGCTTTAATTTCAAAATTAACAGCTGCAAAAGAAGCTAAAAATTCAGTAGTTGAATCTTCGAACAAATCAGACATTATTGCTGCAAATGAAGCATTACAACAAGCATTAAATACTGCAAAGGCTAAAAAATCAAGTATAGATAATGAATTAAAACCTCTAAAGGATGATTTGCAAAGTAAAATTGAAGAGTTTGGACCTATAAGAAATACCAATTTTTCATGAATATCTAGCAAGTTAGAAAATGCTAAAAATAAATTAGCAGAAGAATTAACAAAAGCAGATGCAATTAAAAATAATCCATCAAGTTCTAAACAAGCATTAAAAGATTCTTCACAACAAGTTCAAAAATTAGGAAATGAATTATTGAAGACTATAACCGAAGAATTAGGAAAGGCTGAAACTAAAAATAGTAACATTGGATATAGATTGTTTAAATTGGCACAAGCTGAACAATTTAATAACACTGATGTTGATAAATTAAAGAACGCTTGAGAAGAAAGACAAACTTTAATTTCAAAGAAACAAAAACTAGGCAATCAATCAACAAAAGATTATTTAACCCAATTATCTACAGAAATGAGCACTCAAGAATCTACAATTAAAAAAGTTATAGTAAATATTCAAACACATATAAGAAATAACCTTAACTCACAATATAGATTAGAAGCTGACAAGCTTATTGCCAATATGAAGAGAGGTTATGGAGATAAAGTTGGTATAGAAAGCTTACAAAAATGACAAGATCTTATGGACGATAGTGTGTTGAGTGTAGATGATAGTTTGAAAGACGACTTTAACAAAGCACTTAGAGTATTAGTTGGAGATTACACTAAAAACCCTCCTGTTTCATCGTGATTTATTAATAAAAGAAATAGAAGCATAGAAAATTATCAAAATCTAAGAAATTTAATTCTAGTAAGAGAAAATGAAATTTTATTAGATAAAGCAAAAGACTTAGATAAAAGAGCAGAAAAAACAATTAAATTTGTTGATGAAAATATTAATAGCCTTGACCAAAGAGCAAAAAGATTAAAACAAGAAATATTAAATGCTAAAAAAGACTTAAGTAACTTTACACTAAATCATCAAAAAAATCAATTTACTGCAAAAGATATAACCCCCAAAATTTCTCTATTAGAAAATAAATTAAATGAAATAAATCAATATCTTCTTCCAATAATAAAACAAAAAGCGCAATCAAAGATATCGGAAATTGAAAAAAATAAAAAAGAACTTGAAGATATTATAAGAAGCAATTTTTATCTTTGAGAAAAAAATGAAATTAATAAATATATTAGTGAATTAACAAACAAACAAGTAAATTTAAGATCTAATATAAATTTCGAAAATAAATGAGAATCTATTAAAGATACATTAGATAACTTAAATATTAAAGAAAATGTTTCTTTATTAAAAGAAGTAATAATAAATAATTCAAACGCCCAATATTCAATTAATAGAATATTGTCAACAGTTCCTGAATTTATTAAAGTTGCTCAAACAACAAGAAGCAATAACTTGCGATCACTGTCAGAACAATTTAAATTTGTATTGTTAGACATTAATAAGTATTTAAAAGAAGTAAAGAAAATACTAGACGAAAATAAAACATTAAATAAGGATAACATAACTAAATTAAACGAAAAAATAAGATTGTTTGTGGACAAATCCCGTGAATTAAAAGAATCATTAAGATGATTTGACAGGCAAAATGAAAATATAAGTGATCAAGGGATTAAAAACAAGTTGGATGAAGTCCGTGAACTTCTTAAAAACGTTTAGTTTTGTAAGGTCTTTAAATTAAATTTAAAATTAGTGCAAAAAGCACTAATTTTTTATTTTAATGTTAATCAAAAAAATAATTTCGGATTTTATTTTAAAAAAACTTTATTTTTCTAATGTATTATAATAATAAATTAAATGCTTATTTTTAAGTATTTATATTGCAAGGAAAGGAAAATATGATAAAGAAACGAAGAAGAAAATTAGGATGATTGACCTTTATTGCTGGGGGAGTTGCCATTACTGCTTTTGTTTTAATAATGTGTAGATTTTGCAAGAAACCTAATAAGCAAATAAAAGATCGAACCTCTTTAAATTTATTTAATAATGCGCAAAGTGAATTGAAAGAATTAATTAATTCTCCAATAGCCAAGCTATGTCAAACCGCTATTGAACAAAAAGTTTTAAACGATTCAAATAAAATAAATGAAAAGTCTACTGACGAAGAAATTTCAAAGAATACAGAATATTTAAATAGTTCAATCGCTTTGCTTAAAAATAAGTTAGATCTTGCTAAACAAGAATTAGAAAAATTCAACATGGCTATGCTTCAACTAAGATCATTAGTTAATAATAAAGATAGTAAAAGTATAGATACCAAAAATTCAATCAACGCTTTAAATAATGCTAAAAAATTGCCTTTTAATTCTATTGAACAAGTTAAGAGCGAATCTTTAACTTTGAAAAATAATATTGATGAATTAAAGTTAAAGCTTAAAGAAGTTAAAAAGTCTAGACTTGCAGAAGCCAAGAACAAATTAAAAGCAATTATTGATTCGGAACCTTCTAAATATGTTGATAGTTCTAGAGAATTGCAAATATTTAATGATTCAAATGTTGAAGCAAAATCAACACTTGAAGATATTGATAAAAAGATTGATGCCGCAATAAGAGCAAACAATGATTTAAATGAAAAAATTCAAAATTATAAATCAGCAAGATTATCTGATCTTGAATCTTCAAGAAATGATTTGTCAAACTTTATTGCAAACGATTTAAAAGATCCTGTATATAAAGAAATTGCAGATAAAATTAATGAAAAGATTAAAGAAGTAAAGGGCGTTGGAAAAAATTCGACAATCAAAGAATTACAAGTAGCCATAAAAACTTTAGCAGATGCTAAAGAAAAAGCAATTAAAGAAAAAGCTTGGATAGATAGAGTTGGTATTAATAAAGTTAGAGAAATTCAAAGATCTAAAATATTGTTGGTTGATGCTGAATATATTGCAAGATTTTTAAGATCAGGAAATGGTGAACCATTTAGACAAAAGAATGAATTTAATGAATATTCCGGAACAGAATTTTTCAAATTAAAAAATGAGTTGGAAAGGGTTTCAAATCTTAAAGATTTAAATTCTCTTGACAGTGTTGATTATAAAGTAATATGAAAAGCTAATGAAGAATTAAAAAAATTTATTCCAATAATTAATAAAGCAATTAAATCATATTTATACGAAATTTTTGAAGGCTGATCTGATGTGCTTATTTCAAAATTAAATGTTTGAGATAAATACTGCTATTCGCCAAAATATACTGGTGCAAGTGAAAAATTAGCAGCATTTAAAAAGGAAATTCAACAAAAAAATGAAGAACTAAGGGCTAAGGCACTAGATGCCAAATGAATGGATATTTGAGTAACTTATTATCCACAATTGTTAAAGCTTCTCACTGTTGACATTCGCGATAAATTTAGAGAAGTTTATATAACTGAAGAAGCAAAGAAAAATAATCTTGCATATCAAGTTGTTGATTTATTAAATAAATTAGAATCATTTGATTTGAACAATAGTAAATTAGATAAACAAGAAATTAAAGATTTTAAAGAAAAATTAAGTTCAAAAAATCTAGAAATTGAAAAATATTTAAGTAGCGAAGAAACCTTTAAAGATTATCGCGCATATAACCCTGATTTATGAATGAATAATTTAAGTTATGATATAAAAGAACAAGAAAAAATTTTGAATGATATAAATTCTAAGATTTCTTTAATTGAAGAAAACTCATTTACAAACCAAGAATTAAAGAATACTTTTGCTAAATTAAAACAAAAATTATCAATATTGAATAAAAACTAAAGGATTAGAAACCTTTTAGGGTTAAAAAAGTATATTAAAAATAAGGCTTAAGAAATGCCTTATTTTTAATTAGTCTGGATTATTGAACATCTTTTAGTTCGCTTAAAATATCATCTAATTCGTGGATATATTGAGATGTAAAAATTTCTTTTTCATAAAATTCAGGAAATTTTTTGATAGGAACAATGTTATCTTCATTGTCTTTTTTAACTTTATAAAATTGTCAATTATAGTAGATATTTTCTCGATTTGTTTCACCCGAAAATATAAGTCTATAAGAAATATTTTTTGAAAGCAATTTTTGCAATTTTTCTTTTTGTTTTGAATAATTAGCGTTATTTTGAATTTTTTGTAATAAGGCTTTTGCTTTTTTATTGTAATATTCAAAAACTGAATTAATTTTTTGATCTAGTAATTGTCCGAAATGATAATGAAGATACATCATGTCTTCTTCAGGGTTGAAATAATAGTCATTAGATTTTATTAATTTTTCTTCATCTATATTTTCGGTGTCATGATTTATTGTAAAACTATATAAATATTTATCTAATTTATCTTGTTTTTGCTTTTGATATGATAATAATTCAGTTTCGATATCTTTAAATTCTTTTGAAATAGTTTCGTCATTATATTCAAAAAATAAATAATCTTTATTTGTTTCAGGACTTTTAAAATAAAAATGTTCTAAAATTCTCACTTTGTGAATATAAATTTGTCATGTTCTTTCTAAAACTAACGCCATTTCATTAATTCTATAAAATGCTTCTTTTCTTTCTTCATATTCTTTAAAGAATTGCTTTATTTGTGCGGGTTTTAAATTAATAAATTTTTCTTTTGGCATTTTATATAGTTTGCTAAATGCACTTCAAAGTAATAAAAATGATTTTGGTTCTTTGAATTTTGAGTTTCTAAGTTCCAAAAATGTGTCTTCGCCAGAATCTTTATTTACTTTGTTTTCACCATTTTCTTTAATTTCTTTGCTTAATCTAGCGTGTTCTTTAATTTCTTGTTTTAAATAATTAATTTTAGCATCTGTTTCTTTTGAATATTCATCATAACTTTTTATAGATTGAATCTTTTTAAAATCCTTTTTAAATGATTCAATTTCATCTTTAAACAATTTGTAATATTCTTCCCCATTATCAATATGTGAAAAATAACTATTTTTAATGTTTTCTATTTTTGAAATATTTTCTTTATATTCAGCAATCTTTTTTGATATTGTATTTTCATATTGGCCTAAGGAATTTGAAACGTTTTTTATAGCTGCTGATATTTCAGAATATCTCTCTTTCAAGTCATTTAAATTAAGGCTATTATCAATATTTTTGTTTTTGTTTATACTGATTTCACATTTTGATATTAATGAATCTAGATCTTGCTCTATATTGTTATTTGCTTTTATATTAATTAATTTAGAATGCAAGGATTTTAGTTGCTTGACTGAAGCACAGATTTTTTCTGCATATTCATAAAATTCAATATTTTTTATATAAATGTCATTATTAAGTTTTGAATTCATACTATTTATTTTGTAGAATGAATCCATAAATTGTTTTGTAGAATTTTTAGTTGTTAAGTTATTTTTGATTAAAAAATTTTGTAGGTCATTTTTTTGTTGTATTGCAAAGTAATAATAATGTTCATTTTTATTTATAAGATTAATTAATTTTTCTGCCGATTCTCTTGAATTATTGTATTTTTGAATTCAACTATTTTTGTTCTTGATAATGCAATAGATTGAAGCGGGTATTGTGGCAGCAGAAATAATCAAAGAACTGGATAGCGCAATTAGAAATTTTTTTGATTTTTTCATCTTTCCTCCTTATTTATATTTTTGATTTTGTGATTAGCTTACATATGATTTAATAAAAATTTTATTTACTTATTTTTTAGTAATTCCTTTTGTTTTTGACCTATTATGTGGCACTTTCTGCATCTTGCTTCATATTCATTATAGTCACCAATTAAGTTTTGTTCATTTGAAGAATCTTTTCTAAAACTTGTTGAAGCAGCATGTTGACATACTACACAAATTGCTTGCAATTTAGTTACTCGTTCAGCCATTGCCATTAAGGCAGGAATTGGCCCAAAAGGTTTTCTTAGATAATCTTGGTCTAGGCCAGCTACAATTACTAAATATCCCTTATTTGCTAAGTCATCAGCGACTTCAATTAATGAATTCCCAAAAAAATGGGCTTCGTCAATTATTATTGCCTTATATTTATCTTGTTTAAGTAATTCATAAACTTCATTGATGTTTTTTAATGAATGCGTTTTTCTTCTTACACCACAACGGCTTACTATTTCATCATCAGAAAAGCGATTATCATATTCGGGCTTTATTAAAAGCGGTAGCATCTTAGCATATTCTAAGGTTCTAACTCTCCTTAATAATTCTTCACTTTTTCCACTAAACATTGGTCCAGTGATAACTTCTATCATTCCATCATTAAATGTTTTATACATTTTTATACCTCAAACTTTTAAATAATAATGTTTTAACATTATAATATAATTATTACTTTTTTAAAGGAGTTTTTATGAGTTTAAAAGCTGGTATAGTTGGATTGCCTAATGTTGGCAAAAGTACATTATTTTCTGCATTAACATTAAATGAAGCTGAAATGGCAAATTATGCTTTTACTACAATTGAGCCTAACGTTGCAATAGTTAATTTAGAAGATCCAAGGCTTTATGAATTAGCTAAAATAGTTCAAACAGAAAAAATTGTTCCTGCTACTTTTCAATTTGTTGATATAGCTGGATTAGTTGCTGGGGCTTCAAAAGGAGAAGGCTTAGGCAATAAGTTTTTAGCAAATATTCGCGAAGTTGATGCAATTGTTCATGTTATAAGATGCTTCGAAAATGCTGATATAATGCATGTTAACAATAAAATTGATCCTATTAGTGATTTAAAAACTATTAATATTGAATTAATATTGGCAGATTTGCAAACTATTGAAAATGTAATTAATAGAATTGGCAAAAAAGCTCAAAATTCAGGTGATAAAGAATTAAAATTCGAATTTGAAGTTGCCTTAAAAATACGTAATGCGTTAGAAAATGAAATTATGGTTAAAAATATGGATTTAAGTTCAGAAGAACAAGCTGTGATCAAATCATATCAATTGCTTACTGCAAAACCTACAATCTATGTTGCAAACCTTGATAGTGATAGTTTTAAAAATCTAGAAAGTGCTAAATATTATCAAGAATTAAACAAATATTTGCAAGACAAAAAAGAAATTTTAATACCTATTTGTATTAAATTAGAATATGAATTATGTAAATTTAATGATGAAGAAAAAGAAATGTTTTTAAATGAATATAACATTGAATGCTCCGGATTAAATTTAATTATCAAGAAAAGTTTTTATTTATTAAATCAAGCAACATATTTTACGGCGGGTAAAATCGAAGCAAGGGCATGAGTATTTAAAAAAGGAATGAATGCTGCTCAATGTGCAGGAATTATTCACACTGATTTTGAAAAGAAATTTGTAAAAGCAGAAGTCATTAAATATGAAGATTATATTGCTTTAAATGGCGAAAAAGGTTGTAGAGAAGCTGGAAAAATAGCAATTGAGGGCAAGAATTATATTATGCAAGATGGTGATGTTTGCTTTTTTAGAGTGGCAAAATAAAAATTAAAATTAGGGAAAATACCTAATTTTTTTGTTGTATTTTTTTAAAATCGTATAATAAAAGAGTAATGATACTTATCATAATGATATGATATGAATTAACGAAATATAAAACTTGACCAAATTTATTTGCTATGGTTTTAAAGTTAATTGCATGATTAAAAAGTTTATTGATATACTATCAAAACTAATACTCATTAAAAGTTTCATTACATCATAAAAATTTTATTAGTCTATATATTGCTATATAGTATTTTTTGGTATTACATTAAGTTATTTGTAAGAAACGAACTTTTAAATTTCAACCTATTTTAAGGAACAATTATGAATTTAATAAAAATTGATTTGCATGTACATTCTCAGGATTCAAGCCATACAGGAAGTAATATTTCTCAAGAATCTGATTTAGAAAAATTGTTAATTTTAACTAAAAAAAGAGTCAAAGTTGCTAGTTTTTCTGACCATGATAATTTTTATATTGAAAGTTATTTGAAACGTCTTGAAATAATCAAACAATACAATATTGATATTACATTATGGCCTGGTATTGAAGTTAATTTAAGAAAATATGATGGTCAAATAGGACAAGCTATATTTATATTTGATCCAGATAGCAACTTAAAAGAATTAAATGAAGTAACTAAAAAGGAATTTAGATTCAATAGCAATAAATACACATACAAAGACGCTGTTGAACTATTTAAAAAAAGCAACTTCAATTTTATGGTTTTCCCTCATGCTGGAAAAGGTCAAGATAATATGGAATGGCAAGATATATGCAATAGCCAAGTTGATGGGCTGGACATAACAGATTTTAATAATAGCAATAAAAAGAAAATATTAAAACAAGATCCAAACATTCCAGTTTTATATTTTAGTGACACACATACATGAAAAAAATATCCTCAATATGGTAAATTTTGTTCTTATGTGGAGGTTGAAAATAAAGATCAAATTACATTTAATGAAGTAAAAAACAATATAAGAAATAATAAAGTTGATTATGACAAAGTATAGAAAGGAATAATATGATAAAAAGCCTCAAAATTAAAAATAATAACGAAACTTATGACATAAATTTAAATAATCAAGTTAATGTTATTGTTGGTGAAAAGGGTTCTGGTAAAAGCACCTTGCTTTTGATTTTAGCAGAAGCAATAGTAAACAAAAAATTATTCAAAAACGAATATGAATGATTCAATGAAAAGGCTAAATTTTTAGTCGATTCAATAAATATTGATAATACAGAAATTAAAACACAGGATTTTTCATATATTTTTGATTTAGAAAATAAGCAAAATTCAAAAGATGCAGGAATTTTAGAAATTCAAAAAAACCTAAGCGGTTATATTAGTCAAAATGATAGCAGGAAAAATTCTCTTGATTCTTCTGAATATGTTGAAAAACAAAAAAGTAATTGTATTGATAATTTCACAGAAAAAATTTACTATGATCAAGATCAAAAAATGTTAAAACAATTAGATGATTTTAGATTGCTAGAAGATTTGTTAAATGAATATAGGCGCGAACGCGATAGGCAGATAGCACTTTCAAATGTTTTTAATCAATCTTTTAATTTAAATGAAGGTAGTACTAAAAACATTGCAACCATTGACTATGATTCTTCAAATGAAATAAAAAAAATTAAGGAATTATTGAAATCAATTGAAGAAACAAAAGAAAAAATATATAGTACAAATTTGGTTGTCAATAATCTTTTGGCACAATTTGAACAATCTTCGTCGCTACTTCAAATTTCAAATGAACAATTATTAGATATTCAACAAAGAACAGAAAATGTTATACTACAAAATAACTTATTTCTAGAATATTACAATCAATATAATTTAGTTGTATCCAAGGATAAAAAAGCTCTTGAATGTTTTAAATTATCATTTACAAATGCTAAAAGAGCATATCTTGAAGAATTAAGCAACACTTTAAAGATTCAAAATGATAATGAACAATTATTGGAACATTTTTCTAATTTAGGAAGAATAATGAAAAAAATCAATAGGCAATATAATTCAATAATTGAAAAAGAAATTATTTTAAACTGAACATTAGAAGAAACTGATAAAAAAAATAAGAATATAAAATATAAAATAGAAAGTTTTGCTTTAAGTGAAGAAGAAAAAAATTTAATTTTGAATAAATGACTCGGTTCAACAAATAAGAGTAATACAGTCGCTGATGTTCTTAATGGTAATATTAAAGATAACTTTGATATTAAAAAGATGATTAAGGCATTGATTAAAGATAAAATAAAAATCTATGCAGGGGATGAAGAATATAAAAATCTATCAATGGGCCAAAAGACATTGTTTGGTATAACACACGCGATTGACACATTGAACAATGTTCCTAATGAACAATACTTATTGCTAGACCAAATTGAAGATAATCTAGATAATAAAACAATATACGAAAAAATAGTTCCAATGTTGCAAGAACAAATTAAAAAAGGCAAACAAATTTTTATCGTTACACATAACCCTAATATTGGAACATTGATAAAAGGCAATATAATAACAACAGATATTTTCAATACTAATCTAAACAAAAAATTTGTTGTTAATGAAATAATTCGCGAAGATAATGAAATTGACACACCTCAATCGCTTTATTTGGAGGGCAGTATGAAAGCCTTATATGAAAGATTAAAAGTACATGAAGAAAAAATTAATAAAACCAAAGGAGATAAATAATGATAACAATTGACTATGTATTTACAAAAGATGAAAAGAGATTAATAGTTATTTCTAATGCAAGCGATAGCAAAAATAAATATAAAATTGAAATTGACTTAGACAACCCTTCTGATGCTTGAAATAAAGAAAACATTAATAATTTTATAATTAGAGCAATATCTATTAGTGACGAAAAATTAAGTGAACCACAATTAACAGAATCGGCTCAAGAACAATTACAAAAGGGTAATAAACAAATAGAATTTATTAAAAATTTATTCACAAATTTTGTTGAAAGATATAATGAAAATTAATTTTTATTCAATCTTAAATAAAGCACAAGATTTTTAATAAAAACATTTATTTTTAAGTTGAAAATTAAAAAAACAAGGCCTTGAAGGCTTTGTTTTTTTAAAAATTTTACATTTTTTCAGGTGCTTCAATGTCCATTAATTTTAAACCATCAGCTAAAACAATTTTTGTTGCCTTTACTAAAGCTGCAAGGCTTTGTTCGTTAGGAGATCCTAAAACTTTGGTATTTGAATAAAATGAGTTAAATGCCTTTGATAATTTTAATAGATAAGGAGGCAACAAGTTGACTTTATATGTCTTGGCCATTGTTGAAATTAATTCAGGGTATTCTTTTAATTCTGCAACAAGCTTTTGAGCATATTCATTATCCAACTCATCAGAAGCAATAGGGTTTTCAATTCCTACTTTATCTAATAATTGGCAAGCACGAGCATGAGCATATTTAATGATATACATAGGGTTATTTTCGTTGTTGTCAGTAACTTTGTTAATATCAAAGTCAATTCTTGAATTATATGATCTATCAATTAGAAAGTATCTAATTGCATCATTTCCAACTTCTTCTGCTAGTTCTCTTAACGTTAAAGAAGTTCCTAAACGTTTTGACATTTTAACTTCTTTACCATTCTTCATGATTCTAATTAGTTGGAATAGAAGAATTTCAATTTGGTCTCTTCTATGTCCTAAATCTTCAAATGAGAATTTAACACGTTCTACGTATCCTGAGTGGTCAGCTCCTCAAATGTCAACAATTTTTCCATTTGGATCATTTAATTCCAAAAGTTTTTGTTCGTGGTATGCAATGTCCGCACAGAAGTATGTTGAATCACCATTGCTTTTGATAATAACACGGTCCTTGTCATCTTTGCCACCCTTTGTTGTTTCGAGTCAAGTTGCGCCATCTTTTTTGTATGTTGTCTTTAATCTATGAATTGCTGGTCATACTCTATTTTTGGCATATTGTTCTGCTTCTGATGTAAAAATATCGAATGTTACTCCAAATTTTTTCATATCTTCAATAATATTTTTTAAAGCAAAATCTCTTCCAAAACTTCTAAAGATTTTTCTAATTTCATCAGTGTATTCTACATTTTTGTATTCATCTTTGTGAATGTCATAAAATGCTTGAGCGAATTCTATCATATCTCCGCCATGGTATGAATCTTCTGGCATAGGAAATTCAGGATTAAATATTTGTTGGTATCTAGCAAATATTGAATCTGCCATTTTATTCATTTGGTTTCCATAATCATTAACTAAATATTCTCGAACAACGCGGTGACCTGCTTTTTCTAGAATATTGCACAATGTTGCACCAATAGCAGCATTTCTTGCGTGTCCAATATGCAAATATCCTGTTGGGTTAGCAGACACAAATTCAACATTAATTGCTCCTTTACCTTTTGGATCAATACTTCCGTATGTTGATCCTTCGTTGTTGATTTGATTTATTGTGTTGGCTATAACTGAGTTTGAAACTCAAAAATTAATAAAACCAGGTTCGGCAACCTTTATTTCACTAATTTCTTTAAATTTATTTAAATCAATATTTCTTACTATTTCTTGGGCAATATCTATTGCTTTCTTACCTAAATCCTTTTGCAATGTTAGGGCAATATTGCTTGAATAGTCGCCGTAATTTTTAGATTCGGTAAGTACAATAGGCTTAGAAATATTTAATTTTTCAAGTGCTTCATTTAATGAAGCAATAATAATGTCTTTCGTTGTTTTCATATTCATAATTATAATATAATAAAATTTAAAAACATTATATTTATAGTTATAATATTGAGTATGAAAAGATATTATTTATGTGGCCCAACCGTTTATAACTATCCCCATATAGGCAATTTAAGGCCAATAGTTAGTTTTGATTTAATGATTAGGGCTCAAAGGCAATTGGGAGAAGATGTTTATTTTCTTAACAATATCACTGATATAGATGACAAAATCATTGCTAAGGCAAAACAAGAAAACAAGACAGAAAAAGAAATTTCAAGTTTTTATGAAGCATATTATTTAAATTTATTTAAATTATTCTCAATTGAAATGCCAAATAAAATTGTAAGAGTTACGGATTCCCTTAATGATATGTATGAATATATTCAATCATTGATTGAAAAGGGCGCAGCATATCAAGTGAACGGCAATGTCTATTTCGACGTTTTGAAATTTAAAGATGTGTATGGAAAAATAAGCAACCAGAAATTGGCAAATTTAATTTCAGATTCAAGCAATGAAGTTGACAAGCGTTCGCCATATGATTTCGCTTTATGAAAAGACACAACTGAAGGTATAAAATTCCAAAGTCCATTTGGTCTTGGAAGGCCAGGTTGACATACTGAATGTAGTTGTTTTATAAATAAATATTTTGAAGGCAAACAACTTGATATTCATGGAGGGGGTATTGATTTAATATTTCCTCATCATGAAAACGAAAACATTCAACATTTTGCAATCAATGGCCAAGATATTGCCAAGGATTGGATCCACTTTGGTACATTGAATTATAAGAATCAAAAGATGTCTAAGTCAATAGGAAACATAATTTATGCTGAAGAATTTTTAAAGAATTATTCAGCTGATACTTATAAATTGTTAATGCTATCAACAAGTTACAGCAAACCAATTAATATTACCGATGAATTACTTGCATCGCTTCAAAAACAAATTAATAATATTGAGGCGAATTTTAATAAAAGTCAATTGGAAGATATTAAATATACGTTAGATAAACAAATAATAGATTCAATTTTAAAAAATGTTTCTAATCTTGATTTTTCATTAGCCTTTAAGCAACTTAATGAGCAATTAAAATCACCATTGAATTATGGTAATGCATTTTTTGTATTTAAGGTTTTAGGTTTTAAATTAGTTGAAAATAAAGTAAGCAATGATGATAAAGAACTATATAGAAAGTGAAATATTTTAAAAGAACAAAAACAATACGATAAAGCAGATGAAATAAGAAACATTTTAAAAACAAAAAGGTTGATTTAATGAAAAAAATAGTTTTTGGAAAAAATTCAGTCTTAGAAGCAATTGAATATGAATTTCCTATAGAAGAGATTTACTTGCAAAAAGGACTTGTTAACAACAATTTAAAATTTAAGAAAGTTGTTTATTTAGATAGAAATAGCCTAGATAAATTAACAGAAAATGGAAATCACCAAGGATATGCTGCAAAATTAAAAGATTTTCAATATCATGATTTGGGCAGTATTTTTAAAGACGGCTCAGATAAGGTTTTAATTTTAGACCATATCCAAGACCCTCAAAATTTTGGTGCTATTATACGTAGTGCCAATGTGTTTGGCTTTAAACATATAATCATTCCAAAGGATAGAGCATGTGATGTAACTCCGAGTGTTTTAAAAACTAGCTCGGGAGGTTTTAAAAACGTTAAAGTGATTAAAGTTAACAGTTTATTTGATGCAATTGAGGAATTAAAAAATAAAGGTTTTTGAATATATGCATCAGCCTTAAACCAACAAGCAAAAAAACTAAATGACGCAAAATTTAACGAGAAAATTGCAATTATTGTTGGAAACGAAGGGAGTGGCGTTTCAAAAACATTGCTTAAACATTCGGATGAAATTATTTACATTGAGCAAGATAAGGAATCCGTTCAATCTCTTAATGTTTCAGTGGCAACAGGAATTTTGTTATATACAGTTTTTAATAAGAAATAAAAAAATAACAATTTAAAAAAACAGCGGGTCTCGCTGTTTTTTGTTTATATAGTTTTTTAATATACGCTTAATTTAAAGTAACTTTTGTACCGATTTTTTCTGCTAGAATGTTTTCAAGATCTTTTAGATCACCAATAAATGCTGCTTTTCCGCCTTCTTCAACGAATTTAATAGCTGCTTGTACCTTTGGTAACATTGATCCTGGGGCAAATTGTTTTTCAGCAATGTATTTTTCAAGTTCTGCTTTTGTAGCGTGTTTTAGATCTTTTTGATCTGGCTTGTTGTAATTTACTTTAACATAATCAACTGCTGTTAGAACAATAAAGTAATCAGCTTTTGCTAATGCAGCCATTTTTGCTAAAGCGAAATCTTTGTCAATAACACCATCTACACCTTTAATAAAGCCATTTTCATCTTCAACAGTAGGTATTCCACCACCACCACCAACGATAACGGTAGCACCTGATTCAATAGCTTTTTTAATTTGATTAATACCAACAAAGTTTAATGGTTTTGGAGATGCTACAACTTTTCTGTAGCCTCTACCTGCATCTTCAACAATTGTGCTATTTGGGTTTGCAGCAACTGCTTCTGCTTCTGTTGCAAAGAATGGGCCAATTGGTTTTGTAGGATTTTGGAATGCTAGGTCTTTTGGATCTACAAGAGTTTGAGTTAAAATGTATAAAACTTCTTTGTTCTTTAATCCTTCGTTTTTAAATGCATTTGTAATAGCATTAACCATATGATATCCAATGTATCCTTGGCTCATTGCACCTGCTTCAGGCAATGGAACTAAAGGAGACTTAGGATTAACTGAGTGAGCACTAACGAAACCATTGAAAATCATTCCAACTTGTGGTCCGTTTCCGTGTCCAACAATAACTTCATGTCCTGCTTTTACTAATTCAGCAATTTTGGCTGCAGGAACTTTAACAAGTTCTTTTTGTTCTTCGGGGTTATTTCCTAAAGCGTTTCCGCCTAATGCAATAACAATTCTACTCATATTAGTATCCTAAAGTAGCTAGAATAACAGCCTTAATTGTGTGCATACGGTTTCCAGCTTGTTCGATTGATTTGTTGTATTTTGATTGGAATACTTCATCAGTAACTTCCATAGCACCGGTTGAAACAACTGGGTATTTTGCACCTAAGGTTTCTTTAATAGTTGCTGAGAATTGAGTTTGGTCATCGTGGAATGCTGGTAAGCAGTGTAGGAAGATAACATTTTCTTTTGCTGCTTTGATCATTGCCATATCAACTTGGAATGATTGTAATTCTTGAATACGTGGTTCAAATAGTGAGAAGTCTTCTCCTAATGATACTCATACGTCTGTGTAAATAGCATCTGCGTTTTTAGCAGCTTCTAATTTGTTATCTGAAAATGATACAGAACCACCATTACGTTTGAATAGTTCTTGACATGCTTCGTAAACAGCCTTGTGGTTTGGTCCATTTTTAACAATTTCTCATTGAGCTTTTGGTCCACATAATACAATATTAACACCAAAGAATGCAGCACCGATCATTAATGAACGAGCAACGTTGTTTTTAATATCACCTGAGAAAACAAGTTTCTTTCCTCTTAGGTCTCCAACGTGTTCTTTAAATGTTAAGTAGTCAGCTAGCATTTGTGTTGGGTGTTCAGCATCGGTTAAACCATTTCATACAGGAATTCCTGAGTATTTTACTAATGCATCAACATCTGATTGTTTGAATCCACGGAATTCAATACCATCATAAATTTGGCCTAAAACTTTAGCTGTGTCAATAACTGATTCTTTTTTACCAAAGTTTGATCCTGTTGGGCCAATGTATGTACAGCTTGCACCTAAATCAGCAGCTGCAACTTCAAATGAACAACGAGTTCTTGTTGAGTCTTTTTGGAACATTATAACAATGTTTTTTCCAACTAATGGGCGATTATTTGTGTGTAAACCAGCAAATTTTGCTCTTTTTAGTTCTAGTGATAAATCTAGTAAATAGTTAATTTCTTCGGTTGTAAAGTTTAGAGCGCTGTCTAAACTACGACCCTTTAAATTTAATGGCATATTTTCTCCTTAATTAAAATAAATTTATTTAATAAAAAATAAGTGTGTGTTTTGAATTTTTCAAGTTTTTTAAAACACGTTATAATTATACAATGTTTTAAAAATATAGGTTTTTTATTTTTCATTATAATTTTTATAAAAAAAATAAGATTATTTTGCCAAAATGAAAATTCAAGTGCAACACAAATTTGAAATAAAATTTAGTTGTGGGGCACTTGAATTTTTTTAAAATAAAAAGCTCCACAAACGTACCAAGGGAGCCTTTATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGTTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCGAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAGTTTTCCAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGAATAAACACAAATAAATGAAAAATAAGAAAATCCGATAAATTAAGGCAATATTATAAAAAAGGAGGCAAAAGAACTGCTTCGGTTCTTAGTCGTCTAATAAAAAGTGCAAATTATGTGTTTCCAATATGAACAAGACCAAAAAATATTGATTTAAGGTTGGAATACGGGCATTGAGAGGCAGATTTGGTTCTTGGAAAAAGAGCAAATGGCTATAAAAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGCGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGAAATGGCATGATAAGAAGAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGAAAATCTGCATATGAATTGTTTATGTATGAAAATTTTGGCGAAATAATAAACTAGAAAAATTTAAAAAATATAACATGTTGTTTATCACAACGTTTTTTCTATGCAAAAATTTTAAAAATCGCATAGAAATTTTAATAACTTAATATAATAAAAACATCAAGCACAAAAGTGTTGCACTTGATGTTTCATTCTGGGAAAATAAGATTATTTTTTAATAACCTTATTTTTTATTTCTTTAAAAAATGTTTTATTTTAAATCGCTTTTTAAATCTTTTTTATTCCCAGAATTTTTCGTTTCTAATAAATTTTCATTTAATTTTTTTAATTCATTTAAAATTAATTCTTCTGTTGTTGGAATAGGTTCTTCCTTTATTTCTTCTTCAATTGGGTTCTTTTTAGCTTTTGCATATAGAATAGAATTCTTAATTGTAAATACAATCATTAATCCTATAAAAATAAATGTTGAAACAATAATAAATTGAATTAAAGCACCCAAAAATTTACCAATAAAAATTCCACCAGTTTCTTTTGTTGCATGAATGCCTGGTCAAACCACTCAAGCATCAAGATTTTTCATACCGACTGCCTTTGTGATTGCTGACATAATAACATCATTTGCTAGTGATGATACTAGTGCCCCAAATGAAGCTCCTAATAAAAGCCCAATTGCTAGCATGAACATGTTTCCACGCGAAACTATTTTTTTTGCATCTATATATGACTTTTTAATATAATGCTTTTTTTCTTCTAATTCTTTACTTGTCATATCAATATTATATAGAACAAATTTAATTTGAGTTTTTGTTTTTAATTAATATAATAAAAAAATGGCGGGTAAGAGAGGATTTGAACCTCCGCGGGTGTTTTAGCACCCCTACAGTCTTAGCAGGACCGCCTCTTCGACCACTTGAGTACTTACCCATTTGCGCCATTGCATTAAAATTATATATAAATAATTTAAAAAAATAATAAAAATAAATCAAGGAAATTAATTTTTCCTTGATCTTTGAGGGTAAATTTTATTGATATCATTGCCACTATCAAAAATTCCTAATTTTAAATTTTTAGCATGATTAGAAGCTTCTTGTAATTGTTTGTAGTATTCAATGTTCTTTGTGTAATAAATGCTTTGAGGATTTGTACTAATATAATACATTCTTGCCATTCCAAAGAAAACCAATTGTTCATTTAAATTATAAATTTTTTTAGTTATTTTGTCTTGATAGTAAATAATTGCAACAATTCTTTTATAGTTATCATAAAAATTTTCGTCTTTTGTATTAACCCTTGATTTAGTTTCTTGAGGGACTACTCAAATTTTAGCAGCATTGTTTAGAAAATTCTTTGTATAATTTTCGGCAATTTTTCCATATTTATATTGAATGCCAGACGTTTCTTCAAATTTTCCATTAGTCATTTTATGAGTTTCTGGCGTATCAATTCCATTAAAGCGATATTTATTTCGATAGGAATCTGATATTGTATCTCCATCAGCAATTCTTGCAATATTTATTTCTGTTGCTTTTCCTACTTCAAATTTATTATTTTTTAGTATTTCAAAATCAAATTTAGCTTCATGAAAATTATTGATGTTAAATTTGTCATTATTTGTGTCGTTTTGATTATTTGGCTTAGCTATCTTAGTAAGTTTTTTTATTCTTGACGACAAAATATCAAATTCTTTAATTCAATCAATTTTTTTATTGTTTGAATTTATTAATTTTTTGGCATCAGCAATTAATTGTTTATAATAATCATTTTTTTGCCATTCGTTGCTATTTTCAATTTCCTTTATTTTTTTGTCATATTTTTCTTTAAAATTTTGTTGTTCTTCGTCATTAATTTTTTCTAGTTTGCAAGAACATGAAACTATTAAAATTGGTAAAATTGTAGCAATAGAGCCCAATGCAAGATTCAATATTTTTTTAGTTTTTCTTTCCATATTTATTCAATAAGTCCAACGTACATTTATATATTGCTTGATTTGAATTTGCATAAAGGATTCTTGGATTGTAATTTTTGTTTTCTAAAAAATTATTATTTTTTAAAAAATCATTTAGGGCTTTTGCATTGGCGATTTGCAACTCAGTATTGAAATTGATTTTTTTAATGCCTAATCTAATTGCTTTTTTAATGTCATCTTCTTTGATCCCGCTTGCTCCATGTAAAACTAATGGAATTTTTATTTCTTGATTTAATTTGTCTAATAGTTCAAAATCTAGTCCCTTTCAATTACTTTCATATTGACCATGAATATTTCCAATTGCTACTGCTAATAAATCAATTCCAGTATTTGCTAATTTTTTAGCATCTTCAATGTTTGTTTTTTGAATATCAAGTGATTTATTTTTACTAATATCTAGTCCAATTCTACCCACTTCTGCCTCAACAGTTATATCTAATTTTTTTGCAATGTTAACTATTTTTTTTGCGTTCAATATATTGGTTTCAAAATCTTCTTTGCTACCATCATACATTATTGACTTAAAGCCGGCATTTATAGCATTGATACAATCGTTATAATTACCATGATCTAAGTGCAATATGACATTAGTTTTAATTTCTAAATCTTGAATAAGACCATTAACCAAATTGACAACGGTTTTATAGCCACCCATGTATTTAATTGCAGAAGGAGAAGCTCCGAGTATGATATCTAAATTTGCTTCTTTTGTTGCCAATAATACGAATTTCGCTCATTCTAAATTGTTTATATTTATATGCAAGATTGCATAATTTTCCTCTTTTATTTTTTTAAGTGTTTGATTTAAATTTTGTATTTCCATATTTTTTCTCTTTAATTTTATCATTGCAATAGTCATTTTAAAACAATAAAAGAAATAAAAAAAACTAGCATACACTAGTTTTTAAATTCAGGTCTTTTAGAAGTTCAAAGTCCATTTACTTTTTTAAAAAAACGCCCATCTATTGTTAGTAGTTTATATAATTCACCACGTTTTTTTATTAATAGCTCTTCATCAGAAATACTAGATGTTAATTCTTCTTTTCAACGTTCAAGTAGTTCGTCTTTTACATAATTAAATAAAGTTTCAAAATCTAGTGCATCGTGTCTATCTAAAATTTCTTTAGCTATATCAACTTGCGATTTATATTCCATAATTACCTCATAAAATTTGTTTCTTATTATAACACTTTCTTACAATATATCTAATAAGCAATTCTCTATATATTATATAGATATAACTTTCATAATGACAAAAAATGTACATAATGGCGAAATTTTAGTTAACTCTAGTTAGATTTTTTCAAGTAAGTATATTGTATTTTTACTATAGACCATGAAAATATTTTGAATTTTGTTTTAAATTCAAAATATGGAAATAAATAATTGTTTTATGTAGAATCTTTGTATATTTTAACTTTTTTAACATTAGCCTGCACTAATTTTAAAATATGATGCATTTTATGCATAAAAAATGTTTTAAATTCTTTGCAAATTTATAAACAAAAAAATATAGTATAATTTATTACGTATAAATCATAATTAATGCAATAAAAGTGCATTTGTATTTATACGCTATAACATCTATATTTGAAAGGAATAAAAAATGTCTGTATTTGACAGTAAATTTAATGGCATTCACGTTTATTCAGAAATCGGAGAACTAGAAACTGTTCTAGTTCACGAACCTGGACGTGAAATTGACTACATTACACCAGCTCGTTTAGACGAATTACTATTCTCAGCTATTCTAGAAAGCCACGATGCTAGAAAAGAACACCAATCATTTGTTAAAATAATGAAGGACAGAGGAATCAACGTTGTTGAATTAACTGACCTAGTTGCTGAAACATATGATTTAGCATCAAAAGCAGCTAAAGAAGAATTCATTGAAACATTCTTGGAAGAAACAGTTCCTGTTTTAACAGAAGCAAACAAAAAAGCTGTAAGAGCATTCTTATTAAGCAAACCAACTCATGAAATGGTTGAATTCATGATGTCAGGTATTACTAAATATGAATTAGGAGTTGAATCAGAAAACGAACTAATCGTTGACCCAATGCCTAACTTATACTTTACACGTGACCCATTTGCATCAGTAGGTAATGGTGTTACAATTCACTTTATGCGTTACATCGTAAGACGCCGTGAAACACTATTTGCAAGATTTGTATTTAGAAATCACCCTAAACTAGTTAAAACTCCATGATACTACGACCCAGCTATGAAAATGTCAATTGAAGGTGGTGACGTATTTATCTACAACAACGAAACATTAGTTGTTGGTGTTTCAGAAAGAACTGACCTAGATACTATTACTTTATTAGCTAAAAACATCAAGGCAAACAAAGACGTTGAATTTAAACGTATTGTTGCAATTAATGTTCCTAAATGAACAAACTTAATGCACCTAGATACATGACTAACAATGTTAGACAAGAATAAATTCTTGTACTCACCAATTGCTAACGATGTATTCAAATTCTGAGACTACGATTTAGTTAACGGTGGAGCAGAACCTCAACCACAACTAAATGGATTGCCACTAGACAAATTATTAGCTTCAATCATTAACAAAGAACCAGTTTTAATTCCTATTGGTGGTGCTGGAGCAACTGAAATGGAAATTGCTCGTGAAACAAACTTCGATGGAACCAACTACCTAGCAATTAAACCAGGACTTGTTATTGGTTATGACCGTAACGAAAAAACAAATGCTGCTTTAAAAGCTGCTGGTATTACTGTATTACCATTCCACGGAAACCAATTATCTCTAGGTATGGGAAATGCACGTTGCATGTCAATGCCTCTATCACGTAAGGATGTTAAGTGATAATTTATAAATATTTATAATAATATTTAAATTTATATAAATAATAAACGGACTCACAATAGTGGTCCGTTTATTTTATTAATATACTTTAAATAAAGATTTGTTTAGATTTGGTTAGTTTTAATAATTATTTTTTATTCTTTCATAGTTTTTATGATTTTTTTTGGCATAGGCCGTGCAAATCATATCGCGATCAATATTCATAATTTCGCCTAGTCCTAAATAAACACCGTATGCTTTTTTTATTAATCTTTTATTATGGTGCAAAAACAACTTAGTAAAACATTGATATGTATAGGCAAGTTGCAAGGTAAAGTCATTATATTTAATTATTGGCTTTATATCAAATGAGGTTATGTTATTTTTTAGCGCAATGCTAGTGATAAAATGAATTCCATCCGCATATTCTTCTAACATTGAGATTTGGTTTATACTTTTATCTTTTTTTCAATATTTAAATAGTTTTACTTCGTTTGCAAATTCTCCTAGTTCAACAAGCAAAGCAATAACCATTTTGATTTTGATATTCAAAAATTCTGTTTTTTCAATACTATTTGCAAATGCAAGGTCTAATTTCTTTTGGGCTTCAAAAACTTCTGTTAAATTCATGTTTTGAATTATAAACTTAAAAGTTAAAAATAATTCCATAATAAATTATTTTTTTTTATTATAATTATTTGTATGCCCCGATGGCGGAATGGTAGACGCAGTTGACTCAAAATCAACCGGTGAGAGCCATGCTGGTTCAAGTCCAGTTCGGGGCACCATATCTTTTAAGACTATTTAAACTAGCAGCAATGCTAGTTTTTTTGTATTTTTTATATCTAAAAAAAGAATTTGCTAGTTATAACGAAGCATTTTACTAAAAAATAAATTTATCAATGTTATACTTAAATAGTTAATTATAAAAATATGGGGAATTAAAAATATGGATAATAGGAAGCAAGAATTAAAACAATTAAAAAAAATACCGCTGCAAGAAAATTGACCTAGAATTTTAATATTGCTAATATCTGCAATTGGTTTGCTTGTTTTGATTTATATTACATTGGTCTTTAAAAACAAATATGAAGAATCATTTGAACTAGAAATTTTTAATGATATTTTTGTAAGTTGTTTAGTAGGAATATTTTCAGGAGCATTATTAGTCTTGGCTTCCTTTATATTACTAGATGTTTTTAAAAGAAATAAAATCAAAAATTTCTTTGAATATTATTGTTATCTTTCTTCATTAAAAAGTAAAAATAAATTTTTATTTTTTAAAAATTATGATTTAAGAGAAATAATAAATAATAAAAATCAACTAACCAAACAAGAATTTATTTGCTTTGTTGCCGAAAAATTAAACTATACAATGCCTTCTATTGAATATAAAAACTTAGTTAATGAAATTAACAGTGATTTTGCCAAACATTCATTTTTAGATCCGGATTTTAAAAAGCAAAAGAAAGCAACACTTACTAGGTTAATTATCTTTGAAATTATCATTCCTTTATTTATTTCTATTGCATTGATAGTATTAATTGCATGTCTTTTTAATAATGATGATCAACCAATCAGGGCAATTTATAGGTTAATAATTATTTTAGTCAGTGCAATAATTGTTTTAAGCCTATCGATTTTAATTTATGAACTATTTATTTTAAGTAAAGTAAAAAATTATCAAAATTTCAATAATTTATATTTACTTTCATTTAATAATTACTCATATAAGTATTTAAATTCTAGTTTAGTAAGATAGCAAAAGTACAATAAATTACAAAAATGTTATTTTATTTGAAATACTTTACCTAAAAGTTTTATAATTATACGTTTTAATAGGACTTATAAACGGACAATTTATGGTTTTATTAGTAATAATGAATTTTTGTAGCAATTTAGGTCTAAATTGTTAATTTTTTGTCTCAATATTAATTTAAATATATTAAAATTATCTTTGTGGAAACACAAGATTTATTTAACGCTATTAAATTTTATAAATTAATCTCAACCCTAGTAACAAGCTAGTTATTTTTTTGGTTGAGATTTTTTAATTTATTTTAATTGACAAATATTAAATTAAAGAACATTTTTAATTATTAAATTGAAAGGAATTATAAAAATATGAAAAAGATAAACAAAATTCTTTTAGGGCTAGCACCTGCTGCAGCACTTATTGCTGCATCAGGACTTTCTGCTGCTTGTGGTCATACAGGTGGTGGTTCAGGAACTAGCTATGGATTTGACCAAACAGATGATGGAAAATTAGTATTAGCCTCAGGATTTTCTAACACAAATCCACAAGGCATTGCCTTAAAAGCAGTTGTTGATGCATACAACAAATGAATCAAAGACAATAATAAACAATCAGAAGGATATTTACCTGTTGAAGTTAAAATATTGGCTAATGGATATAATACTAACACTTTAAGAAGTGACTTGGATGCTAAAAACTCAAATGACTTTTATAACATGACATTAAATTATCCAGCTGCTGCTTCGATATTGTCTCAATACAAAATGAATTTAGCTATTTCAAAAGAAGATTATGATAAATTCGGTATTGAAAATGGATTTAATTTAGGTAATGATTTAATTGGTGGAAACACTAAAAAAGAAAAATGAGTAGTTCCTTTATCGCGTTCATCAGAAATGCTTTCAGTTGCTAAAGCATTAGTAGGAAAATTCATTAAAGAATTAAAAGACTTGGGTGTAAAAATTTCTGAAAAAGACAATACTAAGGTTAACGAATATCTAAAATATTACACAGACAATACTAATGAAGCAAAAGGTGTTGATGAAACTTGAGCACATGCAAAAACAGAAAAATTAGATGAAATTAAAGGAAAAATACTTGAAGCATTGCCAGAAATGTCAGATCAAATCTTTAGAGATTATTCAAGTATGATTAATTTCTCAATTCAAGCAAAGAAAATGTACAACAAAGATCCTTTATTAAATGTTATTGGTTTTGACTCACTACCAAATGTTATTAATACAATGACAACTTCATTAACTGGTGGAAATATTAATAAAGGATATATAAAACCAGATGAAGATAAAATAACAACAGGCGGATTTGACTACGCAACATTTCTAAAAGATGCAAATAGTGAACAATCAAAATTATTCAAAAAGATGGTTGACTTAATTTTAGAAGGTGTTAAGGAAGGCGCTGTTTGAATTGGTGGTGCAGGCGCTTATGGTTCAAATAACTTAGTAAAATATCAATTAGCAATGAGCCTTGGTTCAACTGCTGGATACGAACATACATTTTTAAAAGATGATAAAGAAAATCCATTAACAAACTTAAAAATAAAAGACACAACTACTTTATTAGATAATCAATCTACATTAGAAGTTTCTAACAAAAAACCTAATGCATCAGATAAATGAATTATTCAAATTAAATCAGGTAAATACACAAATGGCGTATATGGCAAAGATAATTGAGCAAGCGCTAAAAAGCATGATAAAAAAATAACCCAAGAAGCTGCTGATATAATTAAGAAACAAGTCCAAGGTCAAGAAGGATATTTAGTAGAATTATCTTCAACATTTGAATATAAAAATAATAAGATTTCTCTAAAGAAAGGCAATAAGGAATTAGATGCTGTTTATTTAGGAAAAATTTATGAAGGAAACGAAAAAGAATACTTCTTCTTAAAGAAATCAGAAGTTAATGAAGAAATAGTTTCACCAAATAAAATTTTGAATCAAGATGATGCTGATTGAATTTCTTCACCATTGGCATTTGATAAAAACGCTAAAAAAGCAGTATTTATTCAAGGACCTTCAATTCTTCCAATTCACGCTAACGAAAAAGAAGACAAAGCAACTAAATTATTCATCAACTGAATGTTCCAACATGATTTAGAAAACTTTAAAATTGAAAAAGTTTCTAAAGGAAAAACTATAATTACAACATTTGACGGCAAGCAAAAACCTATTGATATTTTCAATGAATACGGAAGCTATATTTCACCAACTAAGTCATACTTCTCATCAGATAAAGCAAGCAAATTAAATAAAGCAACAAAAATAGCATTTGACAACTTTAAACAACTTGACAATAAAGATAGTGGTTATATTCCATCAGAAGATATTGCAACTTCATTATCAGATGTTTTAAGAGAAGCAATAACAAGTGCTGGAAAAGCAGCATTATCAAAAGCAAACGCTAATAACGAAGTATTTGAATATAAGGAATTTGTTTCAAAAATTTTAGGTCAATTAAGTAACCAACTTTAATAGATTTTTATTAAATATTAATATTTAAAAAACATAAAAACAACCAAATTTGGTTGTTTTTATACGGAAATTAGAGAAAAATATAAAATGAAAAAATACAATAAATTTTTATTAATTTTAGCCTCATCTGCTGGCTTAGTGGTCGGGGCTCCGTTACTGTCAGTATCATGCAAGACAGTGAATAAAGAAAAAATAAATTTATCAAAATTTGATGTAATAAAGAAAAATTATGAAATTCCAGTATATGAATTTTCAAAAGTGGGAACAATTTTTAATAAATCAATAAATTATAGAATGTATGTCACTGATGAAAAAGGAAATAAAACTATTAAAGAAGTTTCTCAATCATTATGGGATTATTATAATTATATTGAAGGCGAAGTTACTAGAATTTTTGATGGCGACACCATTGAAATAAAAGTAACAAAGCAACCAAATCCTAAACCTGGTGGTAAAGTTCTTAATGTTCCTACAACAATGAAATTAAGAATTCCAATGATTGATACATTAGAAGAAAATACGCCAGAAGTTGGAAAAGAAGAAAAGGAATTAGCAAAAAAAGATCATGCATATGCCGAAAAATTAATTCCTATTGGTTCAAAAGTTAGAGTAATTTCAAGCAATTGAAGTGAAAAAACATATGATAGATTTGTTGGATATGTCTTCTTTGGCAAAAATTTTGAAAGACAATTTGATATTGAAATGTTAGCAGGTGGATATACTCTTGCTAGATTAGATACAACAAAAGTTATTTCAGAATTTATTGCTGATTTATCAAAAGAAAGCACTGAAGCAAAGGAAATAAGAAGTTACTTGTTGCCTTATGCTGCATATGCAATTAATTATGGAATTTTAAATAGAAAAGGATTTTATGGTGAAGTTGCCAACATGCGTTCTCCATATGAACTAAGTTCAAAATATTTGGAACACGGACAAGGCATGGTTGATTTCTCACTTTCAATACTTCATTATAATTATTGAAAGAAGCCTAAATTGGCAACTAAAAAGAATAATATCTATAGATATTTAGAACAAATTAATCAAAATAAAAAATAAAGGGAAATTCTATGAAAGAAACAAAATCAAGAGAAGAATTATTAAAGGATATTCATAGTTTTTCTAAGCAACGTCATGAAATTAAAAAGACATTGCCTGCAATTGAAATTAAAAATTTAGTTATTGACTTTGGTGAATCACTAGCAGTCGATAATGCTTCATTTTGCATTAATAAAGGAGAATTAGTAACATTATTAGGACCATCTGGTTCTGGTAAAACAACTACCTTGAATGCCATTGCGGGACTTTTAAGACCAACAGGGGGAAAAATTTTCTTTTCAGGAATTGATGTAACAAAATTCTCACCTCAACAAAGAGAATTAGGATTGGTGTTCCAAAACTATGCACTATATCCTCACATGTCAGTTTACGAAAATATTGCATTTCCTCTATACAATGACAAACATTGACGTGAAGAGACTCTAGAAAAAACTAAATTATCACAATTGAAAATTTTTGAAATCATTTTAAATGCTTATAAAGTTGATGAAAAAGCAATTGAAGATTTAAAGACAAAATGCTACTATTCAATTGACAACCCTAAAGAAACTAAGAAGTATTTAAATGAATTAATTTCACAATATAACGATATTATTGATGAACAAATTAACAAATTAAACTTTTATAAAACTAAAAAAACAGCCGAAGGAACTTCGCTAACTAAAAATGTTTTGCAATATATTAAAAAACTAGGATTGCAACGCCAAGCAGAAATTTCAGAAGCTAGAGAAAAATTTTTGGGTGAAAAACAAAAACTTGATAGAGAATTTGAATTTACTCATGACAAAAATATTTTAAAAAATATATCAGCATTAAAATCAGAATTTTCAGATACTGTTTCTAAAATTAAGTCTAAAAGTGCAGAAGAGATTGCTAATGCTAAAAAAGAATATTCTGAAAAATATAACGAAGCGTTAGCTCGTAATACTCAAGCAATTAAAGATGCAAAACTAGAACTAATGGAAAAGAAACACGAACAAGCAGAATCTCCATTAGTTAATAAAATAAAAGAAATAAAGAATAGATACGTTTTAGTTTCAAAAATAACTAATGTTGAATATACAGAAGCAGTTGAAAATGTTTTTGGTTCGTTGTTCAATTCTCAAAATATAACAAAAGGTAATTTTGATACTAAACTTAAGGCTTTAATATTTGCTTTAGATCAAGAAACTCAAAATCAAATTTCAGAAATTTCTAAAGATGTTTTGACAATTGATGAAGCAATAGTAAAAGATGTATTAGATGTAGCACAAAGAGTTGAAATTACAAAGAACCTAGCAAAACGTCCTACTCAAATGTCCGGTGGTCAACAACAACGTGTTGCTATAGCTAGAGCAATAGTTAAAAAACCAAAAATATTATTATTGGATGAACCATTGTCAAACTTGGATGCTAAGTTGCGTATTTCAACAAGAAAGTGAATACGTTCAATTCAACAAGAATTAGGAATTACAACCGTTTTTGTTACACACGATCAAGAAGAAGCTATGTCAATTAGCGATAAAATCGTATGTATGTCAACTGCTAAAATTCAACAAATTGGTTCTCCTATGGAACTATATTTGAAACCTAAAAATGAATTTGTTGCCAAATTCTTAGGTATGCCAGAAATGACAATATTTGAAGCACAAATTAAAGACGGATATATAATTTACAACAATAAAAAACTACTTAAAGCTCCTGAAGGATATCAAAAATCTTCTATTGATGTTGGTATAAGAGGCGAATCTCTAATAGAAGAAGAAGAAGGAATATTTGAAGGAAGTATAAAATTAGTTGAACATTTAGGCAAGGAAATTCAAGCTCAAATATTTATCAAAGAATTAAATAAAATTGCAAACGTTTTCTTAGCTAAAAAACACAATTACGAATTAGGTGAAGTTGTTAAGTTAAATGCAAAAAGTGAAGGCTTATTCCATTTATTTGATGTAGACAGCAAAGAACGTGTATAATGAAAACCTATTTTTGAAAAAAATATAGAGTTAAAAACACTGGACTTGCCTTAAGCACATTAAATAACAGGGTTAGTTTTTGAAAGCCCTTCTTATTGATGTTGCCATCATTAATTACATTAACTTTATTTACTATAATTCCATTTATTTTGGTTTTAATCTTTTCATCAACATATCAAGTTGGTGCAACGGTTTATGATAAGGGATTTGGACTAAGAAACTATATTAAGTTGTCAAAAGATCCAATATTCCACATCGCTATTAGAAATACATTGGTTTATGCTATATTAGCTTTACCAATTAGCTTGGCGATTTCCATTTTAATTTCATCTGCAATAAGCTTTGTAATCAAACAAAGTTTCCGTAGATTTTGACAAACAGTATTCTTTCTACCATATGTAACATCAGGAATTGCTGTTTCAATCGCCTTTGCATTCTTATTTAGAACATCAGGCGGATTTATTAACATGCTAGCGGGTAAAAATATCCAATGGCTAGATAACCCAAATGATGGTAATTGAAATGCATTTGTTGTTATTTTAATTAGAGGTGTATGAGGAAATTTAGCCTTTCAAATTCTAATTTTAACAACTGCTATGCTATCAGTTAACCCTGATTTATATAAATCAGCCTCTATTGATGGAAGTTCAAATTTAAAACAATTTTTTGCAATTACTTTGCCATCAATTAGAAAAACAATTACTTTCCTATTTACAGTAGGAATTATTGGATCAATTAAAACATTCCCATTGGCTTTATTTGATAACAAACCTGCTGAAGCAGCTGCAAACTCTGGTATGAGTATCATGCTATATATATTCTGATATGTTCAACGTGGAGTAAATGGGGTTGCTGGAGCAGCATCATTGATACTATTTCTATTAGGCTTATTAGTATCATTTGGTTTAAGAAAATTAGTAAGCTTAGTGTTCTTTGCTAACAAGAAGATTGGAGAAATAAATGTCATTAGGAAAATTGAAAATAAAACATTGAAGAGAAAAGTCGTATTTAAGATCTAAGCAAGAGTTAATGTCGAAACCTTACTCTTCAACTAAGGCTAGTTCATTAATAGCATCTTGAGCATTTAAAATAATAATTTTAGTATTCTTCGCGTTAGTAATAATATTCCCATTCTACTTTATGATTGAAATGTCATTTGTAGAAAAACAATTAAGTACAGATTCAAGAACCACAATACTATTTCCAAGGGCAATAGTAGAAGGTGGAAAGAGCTTCTTCCCTGAAAACTATAGAGATGCTTTTGAATCAGGATTCCTTGAAGCACTAGTATTTACTGCTGGTATCACAACTTTCTCGGTATTAATTAGATTAGGATTTTCGATTACATTAGGATATGCACTATCATTAAGAAACTGAAGAGGAAAGAATTCGTTCTTTATCTTCTTTATATCGCTAATGATATTGCCGGAAGTCGCCTTACTAAGTGGTCAATACCGGTTAGTTGTAGCATTGGGCTGAACTATTGGATGAGGTCAATTCTTTGCATTAGTTATGCCGTTTGCTGCTTCAATATTCTTTGGATATATGTATAAAAATGCATTTGAAGCAATCCCAAACAGTGTTAAAGAATCATCAATGCTAGATGGCGCAAATGGAATCAAATACTTCTTTAAAATAGCAATGCCAATGGTTTCAGCAACAACCTGAACAGTTTGCATTCTTACAGCATTTGCATCATGGAACTCATATACATGACCTGCATTAATATTTAAAACATTAGGTTCACCTTGAAAACCTTTAAACCTATGAGTATTTACTACCGGTAAATCACAAGAATCAGAAATTCAAATTGTTTATACATCAATAAGAATGGCTGCAACTGTAATTGCAATTCTCCCAATGTTTATAATTTACTTCATATTAAAGAAGAGAATTATGAATGCAATTTCAAGACAAGGAAATGCAACAAAAGGATAGAATTATGGAAAAAGACATTAATGTTCAATTAGTAAAATTATCAAAATATCAAAAGAAAAGTAAAATTAAAAAAGTATTTGTTTATATTGGTATAGGATTTGTATTAATTTCTTTGATTGTTATAATGATATTGGGTGTATATGGCATAATTGGTGGTCTAGACGGATTAATAAAAAAAGGTTTATAAAAAACTGCAATAAAAAATATCGCATTTGCGGTATTTTTTATTGATATTTTTTAGTTTGCAAATATAATATTTAAACAAAGTCAATAAAAAATTTGGAGAGATATATGGCCTATGGAACTAAAAATTTATTAATATTTAAGAATAATTTAATTCAAAATAAACAAAATTTAAAATCTTTTGCACTTAAATTAAGCTTTTATGTTTTAAAACAAATGCCACAAATTAAAAGTGTTATATTGGCATATGACAATGAATATTGAAAAAAAACGAGCCAATACGTAACTTATTATTTAAAATCCAGAGGTATAAATGTTTATTTTTTACATAAGAATCATTTAGGAGTTCCTTTTTCATTCTTAGAATTTTCTTTCAAGAAATTAAATGCTTTGGACTTAGCAATATATTTTTCTTTTTTTGACAGTAAATCATGTAAAATTCAAACTATTTTTAAAAATAATCAAGAGATAAATTTTTTTGATTTTAATAATTTCAACCTCAATGAAAATGAATACCAATTTTTAAATGATGAGCCAATATATTTAGATACTAAGATGTTGCTTAATGATTATTTAAACCAAATTACTTCAAATAGAGTAACTAAATATCATAATTCAATCCTTAATTTAATAATTAACAAATATCAATATAATTTTGAATTAATTAAGAAAATTATGGGTTTTGATGATTTGGGATATAAATTTATTAATAAACAAAGATTCACTAATGATACAATTTTTAGAGTTTCTAAAACTTTTTTAACTAGGGGCATGCAGTTTAATTATTTCGCTAGATTTTTTATAAATCAAGAAGTTAAATATTATTTAGCATATAAAAAATTATTTAATTATGATTTTAAACAAATTTTAAATGAACATTTAGTTGCATTATATATTAAATTTCAAAACACATTCTTTAGCTCAATGCTAATAAATGATAATTTTTTTAAAATAAATAAAATTTATATAAACCAACTTTATCCCACTCCTATTTTAATAAAAGTTTGTGAAATGTTTGACATAGAATATAGTTTTGTTTTGCATCAACAAGACATTATTTTAAACGACAATGAAAACGTTTTGTATGTTGATAATAATGATATTTCATTTTTAAGTGCTGAGCATAATTATAGAGATTCATTTAAGACATTTTTTTATATTAATGAAATGTTAAACTATTTTGAAACTCAGCAAATTGATATTTTTGACATTGAAAAAGAGTTGAAATTAAAATTCTTAACTTATAAAGAATTTGAAATTGAATGTAGTATTGCAAATTTAGTTCATTTTAAAGACAAATTAAGTATTCAAAAATCAATATCTCGTATTGAAATTGATCAATTAACAGTTTTTGAAAACAAGCAAGACAAATATATAAAACATATTGCTAAGATAGCATTAAAAACCAATCCTCAATGTTTTATATCTATTAGTTATAATGAAATCTCCTGCAAGCTGAAATTTTCTGTTTTTTATTCGCAAACTACAAAAATTTTCAATAAAATCAAAGAATATTTTAACAAATTTCTTTTATTAAACAAAAAAATTCGCAACTAAGCGAATTTTTTAAATATTATTCTTGAAATAATAATTTAATTTTATTAATAAATTCATTGTAATCAAAAATTTTTAAAACATTGGCATCACTCAATATTGATCTAGCTGCTTGCTTTATTGCATCTCTTAATTGACCACTTCTTCTAGTTGCAACATCATCGGCAGGTTTGTATATTGATCCTTCTTTGATTAAGTGTTTAAAGTTTGTGAATGCTATTTCTTCGGCAATATTTTTTGTTACGTTCTTTGCATCTTGGCTTAGAAATTTAGTTGTAGGAGAAATATAACCACTAAAGATATTAAAAGCATCTATTGCTTTTGTACCATCAGGGAATCTTTGAACATTTATCTTTTTACCCTTACTATCAACTTTATAAAGCTCTAAATTATTCAATGGTTGTGTATACATTCAATTAATAAATAATTTAGTTGCTTTGTCTTCTCTTTTATTTGCATGAACTGGAATTACAGAAGGACCTTGAAGAATGATTCCTCTATATTTTTCATTTTCTGAATATGAATAAGGGGGCGATATTATATCTACATCTTCTTGATTAATAAAATTATTAGCATTTAATACATTGACCTCTACATCTTTTTTGTCAATAAAGAAATATTCGTTATTGTCATTGTTAAAGAATTTTCCTAAATAAATTCCTTGAAATTTAGTTATTTTATTTCCAATTTTTAATTCGATTTTATCTTCAGCTTTGTTGTATGAATATTCAGGCCCTGAAACAATAAATCCTGCCTTGCCTTGTAATTTTTTAGCTTCGTTTGTTGATGAAGAAAATATTTTTTTAATATATCTAGGATTTTGTCCGGCATCTTTTATATCTTTTTCACTAGCATCTGCTGTTCAAATTGTATTCGAATATTTATCATTCAATTGTTTAAATTTCAATAATCATTTTTCATTATTTTTGGGAGTATCGTTTAATATTTCAATTGTTGTGTTTGGATTTAACACTCTGTTTGCCTGCTTGATTGTATAATTGTTATTTTTTCCATTTTCACTAACAAAAGTGTAATGATAAGCAGCGGTTGAAGTAATAACCATTGCTAATTTATAGGTGGTAAAAATGCCCGTTCCTCAAACACCTTGTTTAGCTACTCAAACGGCACCTTCTTGAATACCCTTTAAAATAATAGAAGAAACTTTTTTAAATAATTTCGCTTGCGGGCTATCAGGTTTTTCAATAAAATTGTCATAATTGTAGCCACCAGTTGTTGGGCAATTATCATCTGGGTTTATATAGTTTTTATTGATATCACTACCAATGATTGATGTTGCCATAACATTGATAGCCGAAGGTATTTCATCTATTCCTAAAACATTTAATGTAGGGTCCGCGTTGTATAATCTTTTTGCTTGAATTGCAAAATTAATTAAATCGCTATAGTTGTCAAATAATTTATCTGACATGATTGGCAATTCTTTTAATATTTCAGGTGTTAATTTTTTTAAATTTTCTGGTGTTGTTTTTGAATATGCTCAAATACCATCAACGCCTTTTGCTTCTTGATCGTTATTATTGTAATAGTCTAAATAAGATTGCAATTTGCTACTATTTTGACTATCTAATTTAACTCCTAGAGCCATTAATTCTTTAACGAATTTTCCAACTAATGCTTTAGCTACTGTAGTCATTTCTCCTGAACGAGATAGAGGTATTGCCCACTTTTTATTATATTTGTTATTTGCAATTAAAGTGTTTGCAACATTAAATACAGGTTCAATGTTATATTTTTTAAAATCTTTGTCATCAATTGCTAAATTCATTTTGTGATTTGCATATAAAGCAGCTGCTGATGCGTAATTTAATAAAATGTTGTGAAAATCTTTCCTATTTTTTACTTTTAAATCAAGTGCAATTGAAGATGTATTATATCCTGAAGCTAGTCAGGAAATTTTTAAAGGCAAGTATCCTTCAGATTGCTTGTTATTGTCTTTGATTCATTTGTTGTATGTATCAACAATCCCTTGAATAGCAAGCCCTTGCCAATCATTTTTTAAAAATCCCATTGCTAATACTAATTGTCCATCATCTTCTTGGTCAAATCCGTATTTCGAATTTTCTGTTGTTGAACATGAGGATAAAATCAATGTAGTCGTAGGAAATAAACCTATAGTCATTAAAGTGAATAATTTGCCAAGTCTTTTCATATAATCTGTTTCCTTTAAAAATGTATAAGAAATGAACTACAAATTCATATAACCGTGTTGTTCTCTACTGGTTTTAAAACCATGTAGTAATATAAAATATATTTTAAATATAAACCCAAATTTCTTATTTTAACTAATGAAATAAATTATATATTTTTATTGCATTAATTTTTAGAAAACGAAATTCTTTATGTACTTTTAGAAACAAAAAAATGTCTTATTAAATTAAAAAATTCGCAACTAAGCGAATTTTTAATATTATTCTTGGAACAATAGCTTAATTTTATTAATAAAATCTTTGTAGTCAAAAACTTTTAAAACATTGGCATCACTCAATATTGATCTGGCTGCTTGCTTTATTGCATCCCTTAATTGGTCGCCTTTTCTAGTTGCAATATCATCGGCAGGCTTGTATATTGATCCTTCATTGATTAAATGTTTAAAGTTTGTGAATGCTATTTCATCAGCAATATTTTTTGTTGCACTCTTTGCATCTTGACTTAGATATTTATTTGTAGGAGAAATATATCCACCAAAGGCGTTGAAAGCATCTATTGCTTTTGTACCATCAGGGAATTTTTGAATGTTTGTTTTTTTGCCATTGCTATCAACTTTATAAAGTTCTAAATTATTAAATGGTTGTGTATACATTCAATTGATAAATAATCTAGTTGCTTTATCTTCTCTTTTGTTTGCATGAACTGGGATTACAGATGGTCCTTGAAGAATGATTCCTTTATATTTGTCATTATTTGAATATGAATAAGGTGCTGCTATTCAATCTGCATCTTCTTGGTTAATAAAATTATTGGCCTTTAATACTTTAACATCCACGTCTTCTTGATTAATAAAGAAATATTCATTTTCATTATTATTAAAGAATTTACCTAAATAAATTCCTTGTAATTTAGTTATTCTATTTCCTATTTTTAATTCAATTTTATTTTCGGCTTTATTGTATGAATATTCAGAACCGGAAACTATATAGCCTGCCTTGCCTTGCAATTTTTTAGCTTCATTAATTGATAAAGGAGATATTCTTTTAATGTATGTAGGATTTTGCTTAGCATCTTTTATATCTTTTTCACTAGCATCTGACGCTCAAATTGTGTTTGGATATTTTACACCCAATTGTTTAAATTTCAATAATCATTTTTCATTATTTTTAGGAGTATTATTTAATATTTCAATTGTTGTTGTTGGATCTAATACCCTGTTGGCTTGTTTAATTATATAAGTACTATTTTTTTCATTATTGCCAACGAATGTGTGACGATATCCAGCAGTAGAACCAATATTTATAGCCATTTTATAACCAGTAAAAATACTTGATCCATAAACGCCTGGTTGAGCTACTCAAACAGCATTTTCTGAGATACCTTTTAAAATAATAGAAGAAACTTTTTTAAATAATTTTGCTTGTGGACTATTAGGATTTTCAATAAAATTGTCATAATTGTAGCCGCCAGTTGCAGGGCAATTATCATCTGGGTTTATATAGTTTTTATTGATATCACTACCAATGATTGATGTTGCCATAACATTGATAGCCGAAGGTATTTCATCTATTCCCAAAACATTTAATGTAGGGTCAACATTATATAATCTTTTTGCTTGAATTGCAAAGTTAATTAAATCGCTATAGTTGTCAAATAATTTATCTGACATGATTGGCAAGTCATGTAATATTTCAGGTGTTAATTTTTTTAAATTTTCTGGTGTTGTTTTTGAATATGCTCAAATACTATCAACGCCTTCTGCTTCTTGCTTATTATTATTGTAATAGTCTAAATAAGATTGCAATTTGCTGCTATTTTTACTATCTAATTTAACTCCTAAAGCCATTAATTCTTTAACGAATTTTCCAACTAATGCTTTAGCTATTGTTGTAATTTCTCCTGAACGAGATAGAGGTATTGCTCATTTTTTGTTATCTTTATTATTTGCAATTAAAGAGTTTGCATTATTAAATACAGGTTCGATGTTATATTTCTTAAAATCTTTGTCATCAACCGCTAAATTCATTTTATGGTTTGCATATAAAGCAGCTGCTGATGCGTAATTTAATAAAATATTGTGAAAATCTTTTCTGTTTTTTGTTTCTAGGTCCAATGCAACAGAAGAAGTGTTGTAACCTGCTGCTAGTCAAGAAATTTTTAAAGGTAAATATCCTGCTTGTTTTCTTTCTTCTTTGAATTTTGGATCATTTATTCAATTATTATATGTATTAACAATTCCTTGAATGGCAAATCCTTGCCAGTTACTTTTTGAAAATCCTGTTGCTAATACTAATTGTCCATCATCATCTTGGTCAAATCCATATTTTGAATTTTCTGTTGTTGAGCACGAGGCTGAAATTAATGCAGTTGCGGGAAATAATCCTGCTGCTACTACTGTAAATAATTTGGTAATTTTTTTCATATAATTTATTTTTCTCCTAAAAAAAGTGTATAAAAAACGAACTACAAATACAAACATTTTGTACAGTTATGTTCTTACTAGTTTTAAAAAACGCTAGTAATATAAATATATATATTTTTTATTTTTTGTTTCAAAAATTTTTATTTTTTTTCAGCCAAAAATATAAATTTAAATTTTAAATTTTTGCTTGTTAAAATAAATTAATATATTGTTATTACATTATTTTTAAAAAATAGATTTTTTTATAAAAATTAGTGTAAAATAAGCATGCAATGACTCAGCATAAAAATATTTTTTAAATAATTTTTGCTACATTGGATTGAGAGTAAGAAAGCTGTAATCCGAAATCATTTAAGCAAAAATCTTAGGAAAGAATTTTTAAAGTCCTTGCATATGAAAGGAACTAAAAAATGTCAAAATTTGTTGGCTCAATGTTTAAAAAGAGCCGTAGATATGGAATTTCTTTATTAGAAAATAATAAAGAATTCACAAAAGGCAAAAAACGTACAACTGCCCCTGGACAACACGGAGCAAAAAGAAGCAAACCGTCAGACTATCAACTACACATGTATGAAAAACAAAAAGTTAGATATATGTATGGTTTAAATGAACGTCAATTTAAAAATTTATTTATTAAAGTTTCTAAAAAAGCCGGAGTTACTGGTATTAACTTGCTACAAACTGTTGAATCAAGATTAGATAATCTAGTATTTAGAGCAGGATTTGCAAGAACAAGAGCTCAAGCAAGACAATTAGTAAATCATGGACACTTCACAGTTGATGGATGCAAAGCAAACATTCCTTCAATGATTGTTAAAGTAGGTTCAGTTATTGAATTAAAAGAAGCATTACAAAATAATGTTGCTATTAAAGATGCTATTGAAGTAATGACAGTTGCTCCATGATTAACAAAAGAAAACTTTAAAGTAACATTTACAAGATTGCCAGAAAGAAACGAATTTGCTAAAGATATTAACGAATCATTAATCGTTGAATACTACAACCGTTAATAAACAAAAAAACGAGGTTTGAAAAAACTTCGTTTTTTATTATTTATTTTTAACATAATTAACTTTTTTAAAAAATATAAATTGTTTTTCTTTTGTTTTTGTAATTAAAACAATAAATAAGATAAATGCGGGTATATAAACTAAATTAATTAGATAAATATTATTGCATAAAGGATTAAGCGTCATTGTTATATTTGAAAGTGAAAACAGCGTATTTAAAATTTCAGGGAAAACTATAGAACTTAAAACAATAATCAAATAGTTTGTGAGTCTATCATGACTAATTTGATAAAAAATAATACCCAAAATAAGTGATATTAGATATATTAATATAATTACAAAAGCAAACAATATTGCTTTTTTGAAGTTATTGGTGATTTTTATTAATAAAAAACTTAATAAAAATCAATAGGTTAATCTAAAAATATAAACATAAAAATATTCAATGATTTTTGTATTTTCTAAATATACAGGAGTTTTTGAATGATCCCTTCCATCCTTTATTCTATAATAACTTTGATTAATAAATAAAATCATTATGTTAATTAAAAAAACACTAATTAATGCAATTATAGTTGTTAATATTATTATTAAATAGGTTTTTCTTTTTTCTTTGAAATCTAGTTCGATATTGTGCTTTGTTCAATATCTCTTAGCAAAATAAAAGTTTAAAAAGAGTCCTATTGATAACAACATTAAAAGAAGTTGCGTAGTATATGACGGGGCAATCAAAAATATTTGATTAATTAAATTAAATAAATTAGTATTGTCAATCTCACCATCTACTTTTAATATTATTGATGATATTAAAAGATATATTGTAAAACCACCTAGCAATATTCAATATGGCAAAAATAGTAGTAATTGTTTTTTATTTTTTTTCATTAACAAATTTCTCCATTTCACTATGCATGGTAAATAAATGAATATATAGTTTTAGCTCCATATGCGATAATTAATTTTTATTTTAGCTATTGTCAATATCTAATTATTTTTTAGTTTCTTGAATATAATATCATTTGCTTTTTCAATTCATAAACTCATTTGGTTATGAATGTAATTGTAAAATCAACTAGATTTGTTTAAATATTCTTCATTAAATTTTTTTAATTCGAAACTAAAAGTATTTGCTGCTTCTAATAGTAATGCTTCGTATTTTGTATTATCTAATGTTTTAAATAGTTGTTCAAGTTCCTTTTTTGCATCTTCTGGGTTGCAATATATATAAAAGGGTTTAATAATATTATTTGGAAGTGTTTGAAAATCAGGGGTCGCAAAATCATAAAGAGATTTTTCTATCACCCTAATTATACTGAATTAAAGATATTTTTTAAAAAATTCGCATTTTTTTGATGCAAATTTTATTGCAAACTTAAAAAATGCTCTACGCGCCCGCGTACGTACGGGCGCACCCTTGAATAGGGCAAAGGAAGAACCACGGAAACTACTTACCGTAGTGTGGATTTTCCTTTTTTAATATATTATTACTTGTAATAATATATTATAATAGTATGCACAAAAGTTTGCATTATTAAGTTTTTTTAAAAAATAGCATGCTAATTGCTATCAATTCAATTTTTTATATAATTATAAATAATTATATAATAATAGCGTAGCATTAGCAAACAAGGGATTTTAAAATATATAAAAAAAAGTGGTTTTTATAAAAATACCACATTTAAATTTTGGGGAATATTTAACAAGATTTTTAGTTTTAAATAGTCAATATTATGTCTCGAAAATGCTGTTTTTGTATATTTCTTGAGGTAATGTCCAATAAAAGTTTCGGCATTTCCTCCATAATAATTAGCATTTTCATAGCTATTAATTAAGTAATTTTTGTTGTTTTTTCATAATTTTATTAGACTTTTTATTTCAACAATTTTTATAGCAGATAATTTATTTTCTCTTAAATAAATCAAATTCTCATGTAATTTATTTTCAAAATCTTCATATTTTTTGTTATTTAAAAATCACAAAAATTGAGAAAATTGATTCCCAAATGTATTATCAAATATTCTCTTATTATCCTTATTTTTCTTTCTTGAATACCCAAAAACATCAAATAATATTTTGTGAAAATGGAACTTATCATAATACCTAATAGCATTAATTTGTTCGCTAAATTTAGTTATTATTCTTGCTCCATCTCCTGATAAATTAATAGTTTTTGCATGGTAAAAGTTATTTAAAATTTCGCTGGTTTTAGTGGCTAAGATATCTATATTTTGATAAATTTTTTGACTTTTGTTAAACTGAAAAATAATGGTTTTATTTAATAACATATGGTTATTTTGGTTTAAATATCCAGAGTGAAATCCAACCATTCTAACCATAAATTTTCCCTTATTTAAATTAATATAAGCATCATCATTTTCTAGCTGAATTACATCATTTTTTGAAGGTAAAAATTTAGTATTTTTTACTAAAAAAGTAATGTATTTTTTCTTTAAATAATAGTAGTAAATATCATAAGGAATTTTGGGTTTTAATGCTTCGTTTTGATTTGGATTTCTACGCCTTAAATTTTGGGTTTTTATTGCCGTTAAAACATCACTTAATCAATATCTTTTTTTACCCATTATTTTAAGAATTTCGTTATGATAAAAAATGAATTTTTTGTTAGTTTTTTTGTCTAAATATCTAGTAATATTAATTGTTAATTTTTCTCCATTTATTATTAATGTTTTATGCTTTCTTTCAAATACAATTCAATTACTATATTGATTATTTTCAAACCTTGTTTTAGTACGAAATATTTCTTCTTCGATGTTCAATTTTTCTACTATTAATTGGCCTGAGGATAAGATACTTTTTTCGCTTACACCATTAATATAAAAATCTATTTCCATATAAAAATTAAAACAAAAAAGCAAGAATATTCTTGCCTAATGTTTTAGATTATGAGGTTTTGAGCGGAATTGGCTAGCTTTGGACTCTTTCTGAGATTGGTTATATTCGTTGATTACTTTGGAATCTTGAAGATCGGTTGGTGTTTTATCAAAATCGGATAGAGAAGAGGGAATTAGATGAAATAGATATGTGTTTGTTTGAACATCTAAAATATATTCAAAATTAGTATAATCAATTTTTTTAGCTTTATTTATGCATAACTTTATGTTTTTTAGGGCTTGCTTAGTATGTTCTATATTTGACGTGTTTGTATCTTTAAGAAATTGAGATTTGTTAAAATTATGTTTGTAATAATTTTTAGTAGCCTTTTTAATTTTGTAATTTTTGCCTTTCAAAATCGAATTAGACTGAGAATTAACTAGGCTATCATTAGTGTATAAAACTAAATTGCTTTTTAAGTTATTAGATATTTTTAAAATATTTAAATCAGGTGGAATTGAATTAATCCTTTGTTCTAAATCGCTAATTATTAAAGATTTATCGTTTTTATTTAATGTCAAGGTTTCTTGATAGATGTTTTGATCTACATTGGCTTCTAAAACATCTTTATCAATTATAAAAATATCTTTGGTGACTACTACACTTTGATGATTCATTCAATATTCATTAGTTTGATAAGAACTATTATCAATTTGTAAATTATGCTTTTTTGTAGTTTTGGTAGATGTTTGAAGTCTTATACAATAATAGGTTTTATTTTCTTTATTTTCAAAAATTATCAAAGGATGAATTTTAAATTTGGTGTCGATATTTAGATGAAAAATATCTTCTAATAAAGGATTAGTAATTTTTAATGGTTTTCATAGTTCTAAACTCATATAAAAATTATACAAAAAAAGAGGAATAAACCTCTTTTTAAGACGAACTAAGACTTAAGACCTATCATTAGGCTTTACAAAAAATTAAAGTTTATAGGCTGCCTACTATTCGATTACCTATAAATATCCTTTCTTAAGTTATAATAATTATACTACAAATAAATTTTTAATTTTATAATATTTTCAAAAAATATAAGGGGGTGAGCTTAGATTTTTCCGTTATTTAAAACTTTGCCATCGATCGGCAACTTAAAAATATTTTCTAGTAAAGGATTTTTTATCTTTAATGGTTTTCATGATTCTAACATATATAAAAATTATACAAAAAAAGAGGAATAAACCTCTTTTTAAGACGAACTAGAGTCTAAGGCGTATCATCAAGCACTTAAGATAAAATATGAGCTGCTTGCTTGTTCGATTACTCATATAACAAAATCCTTCTTAGACTATTGGTTATATTATACTACAAATAAACAAAAAAATTAAAAATTTTTCAAATCTTTTTTGCATATGTTTGTTCTTAAACAAATTGATAATCTTTTACTAAAAAAATTATAAGTTAATTTAGTAAGAGATTTTTTTATATTTTTAAAGGATTTAATTAATTAAAAACACCAAGTGCAAGAACACTTGATGTTATTTGCTAATTTACAGAATTTTGCTCATTATTTATTTTTGGTTTATTTTTTGTTTTTTTGTCCTTTTTTCTTTCTTTAAGCTTGCTTTTTCTTGTTTTTTTGTTTGGCGTTTTAAATTTTTTGCTTCCTTTTTGGCTAATTTTTCTTGACGCTTATTTGAGCGTTTTTCTTGATAAGATTGCTTGTTTTCATTTTGACGTTTAATACGTTTAGCTCTTTTTCCAAATGATCAACCGTATTTCCTTAATAAAGATTTAATTATGAAAATAGAAGCAACTAAAAATGTTAATATACCTACAATTACCACTAAAGGAAATCATCAAGTTTTCACGACTTTCTTTATTCTATTTGGGTCTTTAGGTATATCTTCGGGACTGACCTTATCCTCATCATCGGGATTTGGATTTGGTTTTCAATCAGGGTCAATTGCAGGATTATATTCTTTATTAGCCTTATTGATAATATATCCATATCCAAACCCGATTGTTTTGTTTGTTGGTAATAAAAGAATTTTTTTGTATAGAACTTCTAAATTTAGAGAATTATTATATATTTCTCTTTCGAGTTCTTCAATAATAGAGTTTTTGTATTGTTCAAGTTGGTTTTTAAGATCTAAATCCTTAGGCTCGAATTCAATTTCTTTATCCTTACCTTCATATTTAAATGTTTTGCGTAATTGTAGGGCTTTTAAGGCTTGTTGTAATTTTTCATAAAATTCCTTGTTTTGTTTTAGTTTAGGATCATCATATTTTGAAACTAATTCCAATCAATAAGCATCGTTATTAATTGCAAAGATTTGATAATCATTGTTAATTTCTAAACCTAATTTAGATGCAGCATTAACTACTATTTCAATAACTTTTTTTCTAATTTGACTTGGCTTGGTTTCTTGAATAACATTCTTGTTATCCATTAAGATGTAACTTAAATTTCTTAAATTAAACTTACCTCCTTCGCCTAAATTTTTAAAGTTAATTATTTTGTAGTTATGCAAATTAGCGTTTTTAGAAACTAGTTTTAAACCAACAAAACTACTTAAAGCCTTTCCTCTCATTAAATGGGCAATTACTTCATTTAAATTGGCAATTTCAACGTCTTTATTTAATAACATTTCAATTTCTTTGCCAGAAGATTTTGTTTTTAGTTGATATTGCAATAACTGATTTGTAATGAAGTTTATTACTTCTTTTTCTATTGCTTTTCTATATCTTTCATCAATAACATCTTCTGGGATGTTTTTTTCTTCGAAAGTTTTTTTAGAAATATTTAAAATAAATTCACTTTGTATATCCAACGTGCTTAAATCAATAGGTGGATTATATACGTGTCATGCTTCATTTCTAAAATAAATTTCTTTTCTAGAATTAGCATACAAACCTTTACCAATTAATGTTAGTTTAACACCTTCATAATTTTGGTTTTGTTCTGTGCCATAAATTGAGACATTAGCAAGCTCTTCTAGGAATTTATTTTCCTCAGTAATGTTGCTAAATTCAGAAATAAACCAATCTTTATTTTCAATTAAATTTTTGGTTAATGTGTTGCTTGACATATTTGTTCTAATATATTCAAGAACTTTGTCTTTTATGGCTTGTTTATCAGTTAGACCATTAAAATTTTGTAATTTACTCCATTTAAAGTCGTTAAAAATATTAACTGCCTTATCCTCATCATCGGGATTTGGATTTGGTTTTCAATCAGGGTCTATTGCAGGATTATATTCTTTATTAGCCTTATTGATAATATATCCATATCCAAACCCGGTGGTTTTGTTTGTAGGTAATAAAAGAATTTTCTTATATAGAACTTCTAAATTTAGAGAATTATTATATATTTCTCTTTCGAGTTCTTCAATAATAGAGTTTTTGTATTGTTCGAGTTGGTTTTTAAGATCTAAATCCTTAGGCTCGAATTCAATTTCTTTATCCTTACCTTCATATTTAAATGTTTTGCGTAATTGTAGAGCCTTTAAGGCTTGTTGTAATTTTTCATAAAATTCCTTGTTTTGTTTTAGTTTAGGATCATCATATTTCGAAACTAATTCCAATCAATAAGCATCGTTATTAATTGCAAAGATTTGATAATCATTGTTAATTTCTAGACCTAATTTAGATGCAGCATTAACTACTATTTCAATAACTTTTTTTCTAATTTGACTTGGCTTGGTTTCTTGAATAACATTCTTGTTATCCATTAAGATGTAACTTAAATTTCTTAAATTAAACTTACCTCCTTCGCCTAAATTTTTAAAGTTAATTATTTTGTAGTTATGCAAATTAGCGTTTTTAGAAACTAGTTTTAAACCAACAAAACTACTTAAAGCCTTTCCTCTCATTAAATGGGCAATTACTTCATTTAAATTGGCAATTTCAACGTCTTTATTTAATAACATTTCAATTTCTTTGCCAGAAGATTTTGTTTTTAGTTGATATTGCAATAACTGATTTGTAATGAAGTTTATTACTTCTTTTTCTATTGCTTTTCTATATCTTTCATCAATAACATCTTCTGGGATGTTTTTTTCTTCGAAAGTTTTTTTAGAAATATTTAAAATAAATTCACTTTGTATATCCAACGTGCTTAAATCAATAGGTGGATTATATACGTGTCATGCTTCATTTCTAAAATAAATTTCTTTTCTAGAATTAGCATACAAACCTTTACCAATTAATGTTAGTTTAACACCTTCATAATTTTGGTTTTGTTCTGTGCCATAAATTGAGACATTAGCAAGCTCTTCTAGAAATTTATTTTCCTCGGTAATGTTGCTAAATTCAGAAATAAACCAATCTTTATTTTCAATTAAATTTTTGGTTAATGTGTTGCTTGACATATTTGTTCTAATATATTCAAGAACTTTGTCTTTTATGGCTTGTTTATCAGTTAGACCATTAAAATTTTGTAATTTACTCCATTTAAAGTCGTTAAAAATATTAATGGCATTTTCTATGCTGCTATTATCGTTATCAGCAAATTTTCAAGATGAATTAACGCATTCGTTATAAAGAGGCAATAATTCATCATATGTTAATTTGTCTAATTGTTCTTTATCATAATGTTTAGATTCAATTAACCATTTTTTAAAATATATTCCAAGTGTTGATTGTCAAAATGGCTCAAATTTTTTCATTAAATCAATGCCATAATTATCTTTTTTATTTTCTAATTCTTGCCAGAAAAGATTTTTTGGTGAATTTGATTCATCGATAAGCACTAATTTAATATTTGAAATTGATGTAGAAGTAGCAGCATAAACTATTCAGATGCCTTCTTCAGACATATAAGCATTTTCACTATTACCGTTTGATGAAGATAATGGTTCAGGTTTAATTTCACTAGGACTAAGCCATTTGTTGCTTCCTTTATCATAAATTTTGCATTTATAGTATGTAACATTAGATTTTTTGGCAATATTTTTATCTAAGATTAGATTTCTTAAGGCTCCTTTACCAAGGACAGCGCCCTCTGCAAAAATACCAAAATCATTCAACATTTTAATATCGGGATATCAAAATTTTAAATTTTTTGTTATTTCTTTAGCTAAAGTTCCGTTTTTTATTCATATGAGTTGATTTTTAGTACCGGTTTTAGGGTCTATTGTAGGATCATATTTAGGGTTAGGTAATGGATTACCTTCTTTATCCACTAAAATTCTTCCATCTTTTGTTATTTCTTTAGTAATTAGTTCCTTTTGTTTTGGGTTGTTTTCAGGGTCTCAAGCAAACCATTTAAATGATTGGTTGATTGGTTGTTCAACTATAACGAACTTAATTTTATAAGTTGCTATTGGCTCTCCGCTATTATTTGTGCCAGGTTTGAATTTCTTTATTTCAACTATATACTCGTTTTTCTTTCTTGAATTATCGTCATTTAACTCTTCTTGAGGCTTATTTGAATCATTTTTCTCTAATGCTATTTTTTCTTCATCGGATGCCGAACGTCTTAAATCTTTTAAATGATAAACAAAATGTCTATTTAAAACATCAACTTTTTGACCATTTATAAATAAAACTTCATTTTCTTCTAATGTGGTGTCAAAATTTACTTGTAGTGGAGTATGCACTAACCATTTTCCCCCGTAATTACCATTTGCAGGCTCTAATTGTATAGGTTTGTCTTCTTGTAGGTTGTCTTTGAAATTGAAACTATGATTTTCTTGAATACTAGATAGGTTATTTTGATTAAGAACTACACCATATTTAAATCATAATCTACTAACAAATCCTGTCTTGTCTGCATAAGTTTGATAAATTTGGCTTGGAGTAAATCTTACTGGAACATTAGCCATTATTGTCTTGCCTTTAAAAAGACTATAAAACATATGATAAAGATTCTTAATTTGAATTTTACTTTGTTGATTTTTGGCAAAATTATTAGCAAAGTTATAAGTATCTGCTTCATTAAAAATTTTAGAAGCAAAAGAATTAGTGATATCTTTTCCCTTATATTTTAATTGTTTGATTTTAAAAATTAGATTAAAATGATTTCCATCTGAAATAGGTTTGACTTCATAACCATATTTAATAATAAAATTGCCAATTGCTGTTTCATTATCCATTTGTTCAACAATTTCTTGTAGTTTTTCAAGATTACTTTTACCACCTTTAAAAATATTTAAATCAATACCTGTCGTTAATTTTCCCATAGTATCAGAATCTATAATAATTTCTTGACTTGAATTTTGTAGATGAAATGCTGACATGAATTTTTGAGAATGCTTAAAATTATTAGAATTTGCTAAAAAGCAAATTAAAGAATAAACATCTTCAAGATCTATGCCATGTCACTTTGTAAAATCATAATTAACTTCTAAATAATCATTGTATGACAATTTAAATTTATTATAAGCAGTATAACCTTCATCAGAATATGCAATATCAGACGAACTAATTTTTGGATCACTAAGTCTATTATTGAATTTTTTAATATTTATATCATAATCTTTGGTGAATTTTTTAAAAGATTGGGCATTAGCTCAGTTAATACTATAATTAGATTTTTTAATAGTTTGAGTAATTTCGCCGTTGCTATGTGTTTCTTCCTGGCACCCATCTTGATCACACCACTCATTACCATCGCCGTGATAAAAAGTTAAGTCGTTGATATATTTTTTGTAGCCATACAATCAACTAGGAGCTCACCATTTTACATTTGGCAATTGTTCCTTTGCCTCTTCTTGATTTTGTAAAGTAATTTTATAATCACCAAAAGGTTGATTATTTATTTTATTAATATCATTAATAGACAATTCTTTTAAAAATTCTAATCTATCATTGGTATTTGCAATAAATCTTTGATCAGAATAAAGGGGATTACTTGTAGGACTTTTGGGGTCAAAAATAAAACTATCTTGCCTGCTATTTTTTCAATATGTCAAATTTGTAAAAAAACTTTTTAATAAATTTTTGTTGTTTAAAAAAGAATATTGGTCTTTGTCAATTTCTTCATATTTATAACTTGAATAATGTTCATTTCTAGAAAAACTCAAAGAAAATAGAGAAGCCGAAGCAATACTAAATATTCCTCCGGCTATCAATAAGGTTTTTTTAATTTTCTTCATCTTCAAAAAAATCTATTCCAAAAATGTCTTTTTCATTATTTTCTGAGTTGGTTTCTTTTGAAATGGGTTGAATATTTGATTTTGACTTTGTCAAAAAAGTAATATTTTTAGCAATTATTTCTGTTTTATAAATAGTTTCGTTATTCTTTGAACTAAATTTAGCATTGTGTAATTTGCCAACAACTTCTAATAAATCTCCTTTTTTAACATATTTCATAACATGTTCTGCCAATTTATTAAAACAAGTAATCTCGATAAAATTGGTTTTATTTGCTTGTTCAAATAGTGCAATAACAAAGGGCAATGCTTGTCCTTTTTCGCTTTTAAAAGGTTTTCAATTATCATTTACTAGCCTACCAGTTAATATAACTTTATTCATTTATCCTCCATTGCACGCGTGCGTGTATTATGCACGCACATGTGCTATATATTTTTATTTTTCTTCTATTTGTTCTTGGGCAGTAGAATTAGTTGCCCCTATTTCTTGTAAAATGTCATCAAATTCATTTTCACTCAAACTTGAAAGAAAATCATTTCAAGCACGTTCTGAATTTTGAATTTTATTTGCCATTTTTTTAGTTCAATCATTAGTTAATTTATTAACAGCACCTCTTATAGTGCGTTCAAAGAACTTAGATTCTGCTAATATATCAGCCCCGGGATTATCAATTTGTTTTTCTAAATCTCCATTATCTTTAAATAAAGCATTAATAATCATATTAAGTTTTAAATTAATAATAGACATTTCTATATCCATGCTTCTAAATCTTCGATTTATTTGCAATTGATAAGGACTTAATGCACTTCAACTAACTTTTCTTAGTGCTTGAAACAAATCATGTTTTCAATCCCGCATAATTTCTTTTTTCTCATTTGCTAATAAATAACTTCTAATAACATTGTTTAATTCTTTATAAACAGTTTTTCCATTCTTTTCTAATTCTTTTTTTCAACGATAAAACATTCCTACATCTTCAGGATCAGTAAATCGTCCTGAAAATTGTGCTTCAAAATTGTTTTGTTGCTTTTTTTCCTTTTGCTTTTTAGGTTGGGAACTAGTGATAGAATTCATAATGTTATTTATTTGATTATCACTTGTTAAATTAATATTTTCTATAAAGTCATCTTCATTTTGAGTTTGATTGTCATTTGATAAAATTTCATTATTATCTAAATTATCAAAATCTTGATCTAATTCGTTTTTATTAGTTTTCAACTAAACCTCCTTATGCACGTGTGCGTGTATTATGCTCGCACATGTGCTATATTAAATTCATTCTTTATTTAAGAACTTGAAGAATAATTCTTGTTCTAATTTGTTGTAATAAATTTCAACTCTAATTCTTTTATTTGAACTTATTGAAAATAAACATTTTCCATATCTTGCATTTACTAAATATCTAATTTCACTTTGTAATAATTGATTAGAGTTTTTAAATAATTGTTGCACTTTGTCAATATCTTGGTCTTTTAGGCCAAAGAATACAGAATATTGACAGTTTTGAATAATTCTAGATGCATCATCACTTGTGGCATTATTAATAGTAAAATCACCAGGATTTTGGGTAGTAAATATCATACCTGCATTGAATTTTCTAACCGTTTTTGTCATTTGAACCATATATTTTAAAATGGCAGGATTATCAGGGTCGATGTATAAATGGGCTTCATCTACGATGACGGCACAAAATCTAATAGTATCTTCTACTTGTTTTGCATCCATTAACTTAATGTTATGTTCTTGCAAATATTTTCTCTTTAACATTTCATTATGTGTTCAATTATTAAATACTATTTCACTAACAAAGTTTAATAAGCACATTGTTCCTAATTTAGCGCCGATATTATCCGCTCCATTTATTAAGTTTTGCGTATTAAATACTACAAAATCATTATCAAGTTTTATATTAGTATGTCCATTAAATAAGTTTTGATATCTTCCATTATTTTCAAACAAAAAGCTAAACGATTCACAAAGTTCAACTAATATTGCTCTTTTTGCTTCAGGATTGTTTAAATGAAGCGTTTTATAGTTGGACATATATTTTATCAACTCACTTATTGTTGGCCATTCTACGGCCTTTAATTGCTCAATAGAAGCAAGTTTATATATTCCTCTATCTTCATAAAATTCTTTTATTACGTTTTGAAGCAATATTCACTTATGATCTTCTATATTAATTAAAACCTTAAAAAAACTTTCTAATCACGACAAATGGTTGTTAATTAGTGAAGATGTATCATTACCCGTTGCATCCTTTAATGAATTACGGATTTGAAGAGGATTGATTATGGTGTTTTTACCTGCTCCCAAATCAATAATTTGTCCATTAAGTTTTTGAGCTAATTCTCTATATTCTCCTTGAGGGTCAATAACAACAACTTTATTATTTGCTGCTAAAATGCTGGTTAATAACTTCATAGTAAAAGTTGTTTTACCTTTTCCACTAGTACCATTTATAAACATATTAAAATTAGTTCTATTTGAATGATACATATCAAATAATCTAAAACTTAGAGGACTTCTTGTGGCTTCTGAGTAACCTAGAAGTAAATTATTTGAATCATTGTGGTTTTCGGTTTCAAAAGGCCATCCCAAAGCTAAATTAATAGATGTAATTTGATAATATTCTCTTTCTAATCTTTCAAAAGGATAATTGCAAGCCTCAGATAAACCCAAAAGCTGTCTATATTTTAAGTCATTTAAGCTTATATTCTTTTTACTTGCTATATTTCTAGCAATTCTATGCTGTCTATTTAATTCAGCAAGGTCAGTGGCTTTTGATACAAAGAAGAAATTACTATCAAATAGTTTGTATTCATCTTTATTTACTTGATACATCATCGCCATTGTCGCTTGTTCATCAATAGAACCAACCAAGTTTGCTGACACCGATCCTGTTGCGTTAGTAGTATCAATAGATGCCATCATGGATTTGTCCATTTTTTTATTGGCAAAATTGTTATTTCAAGGGAAATTATTTCATATACAAGTTCCGTTAATAGAAAATAAGGTTTTGATTCATTTATCAGACAATTCAAATGGCAATTTGTTTAAACAATTGATTTTTACGTAATTATCATTGATTTGTAAATATTTATCTTTAATAATAATTTGATTATAAATAAATAAGTTATCTAATGAATTTTCAACTCCAAATTTAGCATCTAAATCATTGCAATATTCTTGTATGTTTTGGCTATCAATACCAAACATATTAAGTTTATTAAGAAATGTTAGAAGTCTTGCTTTATGCAATTTTTCTAAACCTATTCCAGTATCTTGAAATTTATCAATTATAACTTCGAGATACTCATTTAAGTCATTAAGATTTTCACCATTTACTATTAAATAAAAGTTATTACTTATTGCTTCTTTATTTAAATTAGAGAAATCTTTTATATTAAGGTCATAATAATTGTTTCACATTTCATTATTATTTATTTGCTTTGCTTGTTTGGCTTCAATATATTTAATATTTTCTTCATAATTTACTTTTTCTTCAAGTTTAACAAAAGAAACTTTGTAAGTAAGTATATTTAAAGTTTTTGCAAAACTATCAATAAATGCTTCACGAGTTAGTTCATCCTCGTTTCAAATATTAAGACCATTTAGTTTAAATACCGCTAAATAGTTTTCTTTATCATTTCTTGGATTATAAAAACTTATTTTTGAATTTTTATTTTTGACTACATATTCATTAACTAAACATGAATAAGGATTTAAATCCAAACTACTTCTAAATGAATTATTTTGAACGTTTTTTACAAACCGTTTTGGTATAGTTCAAAAAACAAACATTCTTCATATTAAAACATATACCTTGCAATTATTCTTTCGATTTGGCATTATTAAAATAATTCCTAATAAAAAAGTAGGTATTCAAAATGCAAAGAAAATTAATTTCACATTTATTAATTGGCTAAAACCAAAACTAATAAAAAATGATAATAATAGAATTACAACAAATTCAATAAGATCTTTTGTTTTGAAAGTTTTTCAAATTTTACTACTTCTATGTTTTATTCTACTTGGCTGTAATTGCGTCATATTACTCCTTATACTTTGCTATTTTAGAAAACTTGCAACTTTATATGCTGCACCTACATTCGTGCCCTTTAATGCTGCATCCTTTACTGTTTTGTATGCTCCTGCCCCTGATCTTGCTCCAGACATAGCAAGTCTTGCAGATTGGCCACCAGAAACTTCACTTCCAACAAAAGCAATTGCAATTTCTGATAATTTTTTGAAAGCAAATGCAGAGCCAATTACCAAGAAACAAGTTGATAATAATGCTAAAACGGCTTTGATAGTAGTAAAGTTTGAGAATGAAGAAAAGGCTCCTATCATATAATTTGTAGCGCTAGTCATAAATGTATTAGCTAAGCCTATAAAACTACCCAGCATCATAATTCCCACGCCATAAATCGAGCAGGATAATAAATTACCAAAATATTTTTCCTTTCATACTCTTATTCTCTTTCCTCCATAATTTATAGAAAATGCAGGAATTAGTGGGAATGAAATAAATAGTAATCAATTAACAAATACTCTTGAAATAATGTCAAACAATACAAATATTAAAGGAATTGATATTAATACACCCACAATAGCTATTGATAAGCCAATAGTAAGTGCATAACCTGTAATGTTTTCTGTAAATTTTCATCAGACAGGAAAACCCAAACCTCACAAAGGATTTGCTATAGGAGATCCGTAAGGGAATGGATCTCAATATCTTGAAGGTACAAAGGCTTTTGGGGGCATCATATTTCTAATAATATTATTTGAAATTTGACCGAAACCAAAATTAAAGAAATCGTTATAATCTCCATATAAGGCTTTTGTTAATTCAATAAAGATAATATTTATAAAGAGAATTACTACTTGGAATAATAGTAGCGTGAGGACGCCTCCTACCGTTGATTTTAAGGCTTGAACAAATATTTCCTTAGCCTTTAATCCATTAGATTCTTTTGAATACCTAATAACAAATACAAAAAAGTTTATTAATCATAATACAAAACTAATAATTAAAAATTGATATCAAGGCCTTGCTACAGAAAATTGGCTATAACTTTGGCCTGGTAATAAATTAAACATTAAATAGTTGTTTAAATCGAGGCTAAACAATTTAAACAAGAAAAACAAAAGATTAAATATAGTAAATCCTATTAATCCAATAGCAGCAAGAGGTACAAACCACATAAGCCTAATAGGCGCTGCGATTACATTTATTAAAGGATTTATTATCGCCTGTAAAAATTTCATATTACACCATTAAATTAAAATTAGTCAATTTAGGTATTGAAATTGTAAAATTATTTAATATTGCATGTGTAAATAATGATGCTTGTGAGCCAACATTAGATGGTTTTTTAACATTAGCCTTAATTAATTCAATTAATGGTCACATTACTACTAAAATAATTAGTGCAGATAAAGCACCTCATACCTTTAAATTATTTTGCTTTCTTTCATTAGGGTCTTTTGTGGCAGCATTTTGACTTCTTGCTCACAATATCATTCCTATTAATACAATAATTGCAACTGGAATAAGGGCTATAAAAATAGACCTCATTATAGTTTCACTAAAATTTCCTAAAAAGCTAAAGCTTTTATCATCGATTTTCATTTTTTTCCTTTCATACTCATTAAAGAGTTATTTTAAAAATTAAAAAAATAAATAGAAAACTAACAATTTGAAAATCAAATTGCTTTTTTCTATCATTCAAAATTAAAAATAAGTTGTGGTGTTTTAAACACCTTATATAAGTTAAAATTCTTTTAAATCAACATTTATATTAAACGCTGAATTTCATTTTTTTAAAACATTGTCTAAAAATAATTTTTGTCAATGTTCTTTATTATTTAAATCTAAATATCCATTTGGTTTTTTGATTTCATCAATGTCTTTATATTTAGCTTTAAAAATGACATTTATATATTTAAAGATATAGTCATAAATATATGAATCTTTTTTATTTCTGTCTAGCATAAGCGTTTCTAAATTATTAAATTCAATTGGCATGTTTCAACCGTTTGCCACCATTGTAGGCACTAATTTTTCATATAAAAATTTATAAGCAAATCTATATGTGTAATCAAAGTCAATTTTGTCACCTATTGTTAAACTCTTCATTGCAAACAAATCAGCTAAAGTTGAATGTCTATCATTAGGATCTTCAAATTCTCCTAGTAGTAAATGATGTCAGTCAATAAATTCCACTAACGCTTTTACTAATTCAGTTGAAGCCTTCATTAATTTTCTTTTTTTATCACTTTCGATTTCACTTCATTTTAATTTATTTTCAGAGTTTATATTATTAAATGATTTTAGTCATTGAATTAAATTCTTATAAACTTTAAAATTTAATTTTTCTGATAAGTCAAATCTTTCCATTACTAAGAAATTAAATGCTACTGGATTGTAGTTATTATAAGCAGGAGTAGGAGTTGTTTTATCTAGACCATTTTTATCAGTATACATATCATAAGTTTGTTGTGGAAATTTATTTCTCATTGTAGGATTAGATCCAAATGAAAAAGGAGCAACAAACCAATCATTTGCTTTATTTTCAACGGTTCATCCTTCATACATAGCATTTTCATAATCAGATATTAATTCTTTAACAAATCCATCCATGTCTTCAATTGATTGGTGGTTAAATTCGTCTCTAATAAAAGTAGAAGGCATTTTTGAATCAGCAGAGTAAATAAGTTTTTTTTCAAGTCATTTAGGATCTTTAGCTAAATTTTCCGTTACATAATTTATATGAGTTTCATCCATTGTTAAACCTTTAAATGCAATATCAGGATCTAAATAATCATCCATTTCTTTTAAAAATCTCATTAAGGCTTCATTATAACTTTCATTATCTTTTGGTTTAAAACGACCGCCTTGGTTGATACCTTCTTTGTTTTCATCACTTTCAGCCAATTTTCTAATAATGTTTCAAATTCCACAAGCGCTTTCACAGTCATTTGTAAAATGGTCAGCATTTAAATTTAAATCCATACCTCATTTGGCTAATTGCTCTTCTGGTCCAAGTTCTGGTTTATTTTCTTTAGGCATTTTTAAAGAAAGTCACATTTTAGGTCTGTATAGTTTTCATTTTTGACTATTATAAATCTCATCAGACAATTCCACGGGGTTATAACTCAATTTGCTAAAATAATAAGTGTTATTCTTTTTTAAATAATCTTCATTTGTCGATTTATAAATAACATAACCTTCTTTTTCTTCTGGTTTAACTTCAGGGGTTGGAACTGGAGCAGGAGCAGGAGTTGGGTTTGAAGGATTTGGATTTACTAAACCCGAATCTGGACCCGGTGTTGGGGTAGGATTAGGATTATTTTTATTAATCAATTTATCAAGTCATTCGCAACTCATTGCCATAATTGGTAAAGTAGTTAAGGCAATTGTAGAGCCTATTGTTAATAAAATTCTTTTTGATTTCTTCATATACTTCCTTATATTATTTATATATTTTTTAAGTCTTATAATGACAATTTTTATTCTTTATTATTTGTTTATTTTTTGTATTGAAATTTGCTTATTTTTTATTTATTTTAAGTATATTATAATTATAAAACTAAACAAGTGATTTTGCAAATTTTCGTCTTTTTCTACCTCATATAAATGGATAAATTAATGTTGAGGCTAATATTAAACAAATTATAAGTAGTGCAATATTGAATTGTTTGTTTGTCCATGACCCTTTTTCAACAGAAATAGTCATACCCGAAAAATAAAATGCTAAAAATAATCAAACGATAATTAAAAATATAAGATATCAAATAAAACTTTTTCAAGTTAGCCAAAAAGGCAATAGTAATTTTTGTTGTTTAGTAATTTTTAATCCTCCTTATATTTAATATTTATAATTTGTTTTATTTAGCGCCTTCTAAAATAGAAAGAGGTTTATTTGTAATTCCACTACAAATTCATAGAGAATGAAAAGTAGGCTGAGGAATGTTTTTAAGGTCTTGTTTATAATTTTTAAGAAATTTAATTCTTTTTGTTAAGAACACAAGCTCTAAATTTTTTAGTTTTTGCAATTGGCTTACAAAACCGCCAGAATTAAAACATTGAACTCCAAAAATTAAAGCAAACGGTTTATTTAGTTCTTGCAATCTTTTAATAATATTTGCTTTATTTCTAAACGGAGGATTCGATATTATTAAATCATAATTTTCAATAGGCTCATATTTATAAAAATCTTGCCCATTATCAATATGCGAATATATTACTTTATAGCCCAATGATTTTAAAACAATTACAAAATTACTTTGCTGTGTATCAAAGGGGCATCAAATGGTTTTTTTCTTGTCTATTTTAAAATTTTCTAAAAAGTATTTAATATCTTTTTCTTGAGTATATCATTCATCATCTATTGACATTTGAAATAATTGAGAATTATTTATTTATCACTCCTTTAGCGCTATTAATTTGGTTAGAATTTGCTAATAATTTATTGCGTTTTTTCAAATGTCTAATTATGATAATTAAAAGAATAATTGTTATCATTGTTTCTCCTACACTATGAATTTTGCTTAATAATTTTTATAACTTGTATTCTTTCTATTTGCACCTCCTTAAATTGATTTTTTGAAATAGCATCTTGAGCCATTATTAATGCTTTATTGTATTGTTGCGCAGAGCCAAACGAATATGTTTTGGCATTCTTCCCTGTAGATTGACTCCCTCTTAATCTATAAAAGTATTTTGACACGTTGTTCCTTATAACTTTCTTGATTTTGATTGGGTATCAGAGGGGGTATGGGATGATTTGTTATATTCGTTGATTACTTTGGAATCTTGAAGATCCGTTGGGGTCTTATCAAAATCGGATAAAGATGATTTAGAAGATTCGTTAGGATCTAAGTTAATTAATGATTTTTCTTCTCATGATCCTAAAAAATCAAATAGTAGGCCAATGCGAATTTCGTGCATTTGGTAAGCATTATAGATTGGATGGCCTTTATGATTTAATTGGGTATATTGAGATATATCAAGGCCTTGTTCTAGACCCTTACGAATTTCGTGCATTTGGCTACCAACGAAAATTGGGTGGCCTTGAGGGTCTAGTTGGTTATATTGAGATATATCAAGGCCTTGGGCTAGACCTTCACGGATTACAGACATTTCATAACCAGCATAGATTGGGTGGCCGTTAGGGTCTAGTTGGGTATATTTAGAGATATCAAGGCCTTGTTCTAGACCTTCACGGATTGCATACATTTGAACTCAATCAAAAATTGGGTGGCCTTGAGGGTCTAGTTGGTTATATTGAGAGACATCAAGGCCAGCTTTTAAACCTTCACGGATTACAGACATTTCATAACCATCATAGATTGGGCGACCTTTATGATTTAATTGGGTATATTGAGAGATATCAAGGTCTTGTTTTAGACCCTTACGAATTGCATACATTTGATAACTATCATAGATTGGGCGACCGTGAGGGGATAATTGGATGTATTTGGATACGTCTAGACCTTCTTCTAGGCCAATGCGGATTTCATTCATTTGATCACTATTGAAAATTTTTTTCTTCATATTACCTTCTTATTTTGTTAATGAATGGGATTTTGATAGGGATTCCGAGGGGGTATGGGATGATTTGTTATATTCGTTGATTACTTTGGAATCTTGAAGATCGGTTGGGGTCTTATCAAAATCGGATAGGGAAGAGGGATGAGAATTATTTGAAGAATAATGTATAGATTTTTTAAAGTTGTAATAATTTCTACTGGATTTTATTTTTATATTTAATCCTAGTTCTAAACCATATAAAAAGAAGTCTATATTGTCTTTATTAAAACCCGATTGTTTTAAATTCCTGCGATATTTATCAATAAATTCATCTATATGCATCTATTTATATTTTTTGATTCTGAGGTTTTATTTTGATTAGGTTGTGAGTGGGATTTGTTATATTCGTTGATTACTTTGGAATCTTGAAGATCGGTTGGGGTCTTATCAAAATCGGATAAAGAGGATTTAGAGACTAATTGAGTGGGTTTAGATACGTCAAGGCCTTGTTTTATGCCTTCACGGATTTGTTCCATTTGGCAAAAATCATAGATTGGGTGGCCGTTAGGGTCTAGTTGGGTATATTTAGAGATATCAAGGCCTTGTTTTAGACCCTTACGGATTTCACCCATTTGAAAGCTGTCAAATATAGGGTGGCCTTGAGAGTCTAGTTGGGTATATTTAGAGACATCAAGGCCTTGTTTTAGGCCATAACGAATTTCAGACATTTGGCTACCAACGAAAATTGGATGGCCTTGAGGGTCTAGTTTGTTATATTGAGATATATCAAGGTCTTGTTCTATGCCAATGCGGATTTGTTCCATTTGTAATCAATCAAAGATAGGTCTATTAAAATTGTTTTGTTTTGCATAAATTGAGACGTCAAGGCCTTTTTTTAGGCCTTTGTGGATTTCCTCCATTTGTCATCAATCATAGATGGGGTGGCCTTGAGGGTCTAGTTGAGTGTATTGGGTGACATCAAGGCCGTGTTCTAGACCTTCGCGGATTTCATTCATTTGGAAGCTATCAAATATAGGGTGGCCTTTATGATTTAATTGAGTATATTTAGAGATATCAAGGCCGTGTTCTAGACCAAGGCAGATTTGTTCCATTTGGTCATTATCAAAAATTGTTTTTTTCATATTACCTTCTTATTTTGTTAATGAATGGGATTTTGAGTGGAATTAAAGAGCTTTGAATAATCATTGTCATAATTTAATAAGAATTTTTATTTGGGACTATTTTTTTGATCATTAGCTTGATTAGAATTTGCTAATAATTTATTGCGCTTTTTTATATGTCTGATTATGATAATTAAAAGAATAATGGTTATCATTTTTTACCTTCTTATTTTGTTAATGAATGGGATTTTGATTGGGATTCCGAGGGGGTATGGGATGATTTGTTATATTCGTTGATTACTTTGGAATCTTTGAGATCGGTTGGGGTCTTATCAAAATCGGATAAAGAGGATTTAGAGGCCAAATGGGTGTATTGAGAGATGTCAAGCCCTTGTTCTAGACCCTTACGGATTGCATACATTTGATAACCATTGAATATTGGATGGCCTTGAAGGTCTAGTTGAGTGTATTGTGAGACATCAAGGCCTTGTTCTAGACCTTCACGGATTTCAGACATCTGACCTCGATTAAAGATTGGGCGACCTTTATGGTCTAGTTGGCTGTATTGAGATATATCAAGGCCTTGTTCTAGACCTTCACGGATTTCAGACATTTGCAAACAATCATAGATAGGTCTATTAAAATTGTTTTGTTTTGCATAAATTGAGATGTCAAGGCCATGTTCTAGGCCAATGCGGATTTGTTCCATTTGTCAAAAATCATAGATTGGATGGCCTTTGAGGTCTAGTTGGGTGTATTGTGAGATGTCAAGGCCTTGTTCTAGACCTTCACGGATTACGTACATTTGACCTCAATTAAATATCGGATATCCATCATGATCTATTTGATTATATTTAGAGACATCAAGGCCTTTTTTTAGACCCTTACGGATTTCGGTCATTTGATTATCATTGAATATTGGATGGCCTTGAGGGTCTAGTTGAGTGTATTGAGTGACATCAAGACCTTGGACTAAACCAATGTGGATTTGTTCCATTTGATCACGATTGAAAATTTTTTTCTTCATATTACCTTCTTATTTTGTTAATGAATGGGATTTTGATTGGGTATCAGAGGGGGTATGGGATGAATTGTTATATTCGTTGATTACTTTGGAATCTTTGAGATCGGTTGGGGTCTTGTCAAAATCAGATAAAGATGATTTAGAAGATTTGTTAGGATCTAAATTAATTAATGATTTTTCATCTCTTGATTCTAAAAGGTCATCTAAATCAAAAGTTTTTAATGCTTCTATGTAATCGTCTTTAATTTCTTTAATATTAATTGGTTCTATTTCTAAATAGGGATTTACATAAACATATTCTTCATTTGACAAATTATTTAGAACATTAAATTTCGTTGTTCCATTAATTCATTGACTTTCATAACCATTAGCTTTTAAATCTTCATCAATTATTTCTTCTAAATTATCCTTGCGAATAATGTCATTTTCTTCCATAAATTGACTTGCAGGATATTTAAGCCAATCGCTTTCATTTATTCAATTATCTGAAGATAGGGTATTAATTATATTAGAACATCCTTCCAAATAGTTTGGACGTGTTTCAGAGGTTATTAGTTTATCATAAATTCATTGATTTACAAAATCATCTGATTCTTCCATTTGTTTAGAAATTTGGCAGATTTGTTCTTTATATAGTTTTTGTTCTTCTTCTGAATTTAAACTAAATAAAGTATTATTAAAATAATTTAGTACCTTATGATCGTTATATCAAACGTCGTCATATTGAGTCATATACAATATATCATTCATCTTAAATGGTTTTATTTCCATAGTTATTTATCTCCTTTTTCAACTAACTTGTTTTGTTTTAAATAGTACTTTGCATAATATTTTTCTGCATCTTCTCTGCTTATGATGAAGGCTCTACCATAGGCGTCAATGCCCACGAATTCAATATCATAAAAATCGTTTTGCATTTCATAAATGTTATTAATGTCAAACGCCCATAAATCTGCTTCCATTCCGTATTTTCTTAACTCTTTGTCTGCTAATTCTCAAGCGTCGTTTAATGGATAAACGTCTTGCGGTTCGTGGAATTTCCAATAATTTAAATCTTCTTGCAATTTTTCAGATTTAGCTCTACATTCTCTTGAACAAAAATCAGATCAAAAATTCTTAACATACATTCCTACTAGTTCTTTTCCACAAAAAGCACAATATTTATCTTTTGTCATGTTTCTCCTTATTGTTTGTGTCTAAAAATGTGTTTAATTATTTCTTGCAATACATTAACAACAATAGAATTTCCAGCAAACTTATAAATTTGACTATCACAGAAATTCTTAATTCTTTCATAATCTTGCTGATCATAGCCCATTAATAGCATTGCTTCTAATGGTGTAATAACCCTTAAATAATATGGTTTATTATCTATAATCATTTTTGCTAAATTGCTATTATTAGGAACTTTGCTAATTTCTACTTTAACAACCCCTTGATTATTTCAACGCATTTGTTTAGTCGTTATAGTAAAGACTTTATTCTCTATTATTTTTATTTTATGGTTTGCTATGGCATTAATCATTGAGGGGCAATTTGCTTTAATAAAGTATTTTGGATTTATTACATTTGGCTGTAAAAAATCATTTATATTTTTATATTTATTGTTTAGACAATTTAATGTATCTAAATTATTGAATTGTTTTAAATAGTTTTTATTAATAGAAACAGTAAAAACTCTATTTCTACTTTGGGGAATTCCACAATCTAATGAATTAATTATTTTATAATGGCTAACATATCCTAGATCATTTAATGCCTTAATATATGCATCAAATATAGGTTTATGTTTTTTGCTTAATACATTTTTGACATTTTCTCAGATAACAATTTTGGGTTTTATTTGAGCTTCTTTAATGATTCTAATTGTTTCCCACAATAAACTACTTCGACTTTTACTTTCTTTACTTCCTCCGATATTTTTACCTGCACACGAAAAATCTTGACAAGGAGATCCGTGAAACAATAAATCAACATTAACATTATTAGGAAGTGAATAATCTCTTATATCTAAAGGTTTGTAAAAATCGTTATATATTGCATTATAAGCATTTACTGTTTTTTTATCACTTTCGACATAATCAATAATTTCAAAATTAGGAGGTTTGATGGTTCTAATTAGAGCTTTTCTAATAGCGCCTATTCCGCCAAATAATTCAACAACTTTTATTTTATTTATATTCATAGTAAAATTCCTTAATTAGATTTTTCAATTAACTTGGAGATGTCTTCGGTAGGATGCAACAAGTAAAGCAATATATCAACATTCAAATCGTGATAATTTGGTATTTTGCGCTCTTTTGCCTCAGATCAAAGAATATTAAGCAAATCGCTTTCGAGATCATTGGTAACATATTGCTCTCACATTAAACTTAATGTTTCTTCTATTTGTTCTTTAATTTGATTTTTTTTATGAGGTATTAATGTACGTTTATAATCATTTTTTAGATTTATATAATTGTTTCAAAGATCTAATTTTCCAAAAACCATCTTCATTCTTTGAATTTTTTGTTCTTGGGATAAATCCGCAGGATCTTCAACATCTTCTGAATAAGTTAAATTGTTATCATCTAAAACTTTTGGGTTTTTATTATTTTGTTTTTTTTGTTTAGAATTATCAAATTTAATAAGTTTTTCCAAACTTCTAATATCAAATAATTCAAAATCCGCATTAGTTAGTAGCTTACTTTCGTTTCTGGTATAAACATAATTATCATTTGGGAAATATTTATAAGCCAATAAGGTTTTAATTAATAAAGGTTTATAACCATTTTTTATAATTATTAAATCATCAGGTTTTTTAGATTTAAGTTCCGCTACTTCCATGACAGGTTTGGATTTTTCACTTTCAGTAGAACTTGAATGGTTATCACTTGTTGTATAACTTTTTGATTTAACCTCTTTTGTTCCGAGCGCTTTTGAAATATCGTTTAATGTTTGCTCATCATTAGAGGCTAAAAAATATACTACTTGGCAAGAGTTTTTAATTACTTGGGCTGTTCCTTCTCCATATATGTTATATTTCTTAATTTGATTGTAATCTTGTACTACCAGCATGAAGAAAATATTTCTTGAACGACAAATTGTAAGTTTATTATCAAAATTTACAACAACAGGTAAATTTCCGAATTCATCCAATAGAAAGAAAGCCTTTCTGTCTAATTTTAGGTTATCATTTTTGTTTGCTTGTTCAATTAACGATTGATAAATTTGTTCTACCAACATACTTACTAAAAAGTGAGATGTAGGTTTATGGTCAGGATAATGTATAAATATAGCAAAAGGTTTAGGATCGGACACTATATCAACTAAATTGAAACTTTCTGTGTCTCTTAAAACCATCTTTAAATTTTCATCATTATTGAATACATTTAAAACACTTTGTGCTTCGGCTAATATTCCACCAAATGTTTCTTTAGGAGTTTTTGACAAATCTTCTATATTGTTATAAATTTTTTTTCAATAATAATTATTGTCATCAAGCGCTCTTTTTTTAGCATATAAGATTCAAGCAGAGATAGTTTTATCGTTTTCTCAGTGAGAAGTATTTAATAATGAAGCTATTGTTTCGAAATTAAAATTATTTTTATGAATAATATTTTTATCATCAATACCTAATAGCAATAAGAAATTAGTAATAACAATTAATGTTTTTTTAGCTTGGCTAATCCACATATTGCCATTAACTGCCTTACCTCAATTTAAACTTTCAATAACATTATTTATAACTTCTAACGATTTATTAATTCTTTCTTCGAAAGTCAAATTCTTGAATTTTTCACCATCAACCGCTAAATCTCATGCATATGAAAGAGGGTTTCATTTTAGAGATAATTTAGGGTCAGAGAAATCAATTACATAAATTTTGTAATCGTTTTCAGTTAATAAGCCTCCTGTAAATTTGATAATTTCTTTCTTTGGATCGCTAATTATTAAATTTGGTTTTTTAGAATAATCAAGTTGTGCCATAAATTCAATATTTGGCAAAATTATTCTATGAGTCTTCCCTGATCCTGGCGGTCCATTTACAATAGAGTTATCATCTTTATCATTTACATATCAATATTTTTGAGTTTTATCAAAAGTAAATAATCAATTTGGTTCTGTTTCTGCATTAATCATTTTTCTAAATAATTTATTGTATTTTGTTTTAGAGCCTTCTCCTAATTGATAATTTCATAATCAATTTTGGCTAGCCTTTGCACCAAATAAGTATTTTGATATCTTTTTGCCAAGAGATTTTATTTGCCTTCAAAAAATGAAACAGAAGACTAATATAGAAATTCCTAGACCTATATAAACAAAAATATCAGGTTTGGTTTTTAATAAAGTAACAGTAGAGTTTCAAAAAAGTTTAAAATGTCCAGGATTTTTATTCTGGCCCATAAAAACATTAATAATAAAAGTTAGAACAAAAAACAAAAATGTACTAACAATTATTGAAAGAAGTGTCTTTCCTATAATTTTTGATTTTGATAATTTTTCTTGTTCTTCCATAAAACTCCTAATGGACAAGTTTGCTACGATAAAACTCTTCTACTAGTTCAAGTTCTTTTAAATCTTTAGCCAAAAATTCTCTTTCCGCTAGATTTCTAAGGGCTCATTCCGATCTATTTAGGCTAAATATTTTTAGAAATTCATTGAAAAATGAAGCGAAATTCTTTCGGCTAGTAAATGCATTATTATTTTGCTTATAGGCATTTAGTAAGGTTTTGATTATCTTATTACCAACTTGTTTTTGAAAATCATCCATTTCATTATTTATAATTTTTGAAATTCTTTGTTTTAGAAATTTATCTTCGATGTTTAAATTATTTAAATCATTAATTTGTTTTTGATAAGAATTAAAGAGCTTTGAATAATCATTGTCATAATTTAATAAGAATTTTTTTATTGTTTCAATATTTTTCTTATTTTCATCAGAAATACGAGAATATGAAATATTTTTTTTGTTTAAAACTTCATCTAAAATACTTTTTGAAGATTTAATGAATTCTTGGCACAAATTACTTTTAGAAATAATTTCATTACTATTAGCAAAATTATTGGCAATTATTCCTACAATTTGTTTTCTTGTATTTCAAATGTCTTTTGTAAGTGCATTTATTTTTTGATAATCCTCATCGTATTTAATAGATAGACCCACTTTATAGCCAAAAACATTAATTGAATTTAAATTAAGTTTGGCTTTTTTGTGTTTATGCTCCTTTTCATATCAAAACATATGAACATGAGGATAATCAGTATTTCCATGTATAGAATAAAAAACATTAACATTTTCTAAATCAAAGGCATTAGCTTTTAAAAATCTTTCAAAATTAGGTTTTAAAACTTCATGTCATTTATCCTTACATAAAACATTATTATCTAAGCCTAAATCACCAGGATTAACGATTAATTCTCAATAATTACCTTTTAAATTTTTTAAATCGTTTTTGACATTTTCTAAATTTACATCCTTTGGATCATTATCTAATAAGTTATAAAGACCTGATTGTTTAGAATTTTGATTTATTTTTAAATGTTGTCTATATCAATTTATTTTATCTTCCTTAGTAAGAAGTAGTTTCATAGCATCATAATTATTTTCATCTTCTTTAAATACAACGCATTTATCTGGTCTTGTAATGTAATCTACCACAAGTCCAGAATCAACTCAACTTTTAAAATCGTGAGTATTACCAAGTTTGGTTTTATACATTGTGTTAGGAGTAATAAAATTAAATTCAGTTCATACTTTATCCATTTATTCTCCCTTCTAAAACATTAGTAATTATTTATATTTATTACTGCTATATATTTATCTTTAAAATTTTGACCGCATATTCGCTCTTATTAATTTGTATTAGTAATTTGACTAATTTATTTATGACTAAAATAGACACTTAGTGCCAGTAACTATCAAAACTTAAATTTAAACGTTTTTAAAAGTAGTAATTTAAAATGTAAATATTATTTGGTGGCTCGCGTAGGATTTGAACCTACAATGCAGAAACATTAGCCCAATGCTGGGAGCCATTTATTACTGCTTATAAAAACCTTTAAAACTTTTAAATTTGCATTAACAAATAACGTTGTGTAGAAAATTTATAAACATTACATTTGATGTTTAAATTTCCTAATTTTAATGTTGTAACTTTTTTTTCTTGACTACGCAATATACCAAACATTTGCAAATATTCAACTCCAAAATAATTGGCTATGGCTTTGTTCATTAAATATATTTTGCTATTTGATCAGTTAAATTTTGCATAATATGCATTTTGTAAAATCACTTTTCTTGAGTTTATTTTACAATGCAATTTTTTAGCATTTAGTTGCTTTGATTTATGCCTATTTATTGCATATAAAATAGCATTACTTAAACCATTAAGAAGAAAGATTTTTAAGGCATCATAGCCATTTAAAATCCAGTAATTTTCCTTAGGGTTTTTTAGTACTATTTTTTTTAATTTTAGAAGTTTATATTTTTTATAAAACTCACCATCTAAAACTTTTATTTCAATAAGTCTTTTTATAGCTGATTTTATAGAAGTTTTTTTAAACCCTAAATTTATTAGATCAATGATTTTGATTTTTTTATTTTTAAAGGATTTAATAACCATAAAAACTTCAAGCAACACTTTATTTGTTTTTAGCTTTTGATAATAACTTTCACTAATAACTATTTTTGATTTTAAAATTTTGCTTATAGCTATGTCGCTTAATTTGTCATATATTTTTTTACTATAATTTCTCCTCCAATTTAAATCTCTTTCATATGCATCCACAAGGCTATAATCGTTGTTTAATTTAGCCTCTTGCTGCATTACCATAAATTCACTATAAGCACCCATAAAATTCCTTTCAAAAATTTTTAAGTTTTTTAAAAAAAACGAAGAGCACGTGGTATAATTTATGTAGATGTATGAAGAGCTGTGCTTGTGTTGTGCTCTTCGTTTTTTTAAACTTATTTTTTTTATGAATGATAGAAAAAAAGCAAGACCTTTATGATCTTGCTTTTTTTCCTTATTTGGAATTAACCCCACATTTTTCTACGCTTCCTATTATTTTGGATTTTTGTCATAATAATAAAGCAATAATTAAAGTCTTCATCATTGACAAAATTTAATAGTTTTTTTATTAGTTTTTGAATAATATAACTTGCTTGGTAATTATCTTCATCATTTGAGTTGCTGAATTTAAATTGGTACTCTGTTTCTTCGTCAAATAATTGATTATCAGCTACAAATGCTTGGTTTATATCGCAATATAAACCTTGAAAATTATCAATGTCATATTGACTTAAATTATTTGATATTGAAGTTAGAAGTACTTCATTATCGTGTTTTATTTTTGAAGCCTTTGCATAAATTGTAAATTCTATTGTTCTAACAAGAGGAATTAATTTATATTCATCAACATCATTCAAAGGTAATAGTAATCTAGAACAAATCATATACTTTCTCCAATCTAATAAAAAAACCACAAGACATAATACTTCTCGATATGGCTGCTATATTTCTATCCTGACCAAGTTACAAGGTTATCATTCTCATGGCATAATAATTATACTAAAAAACAACTATTAATACAAAAACTATTCAATATTTTGATTAATTTAACAATTTAATTCTTATTTTTTGTTGCCCTTGTAGTTGCTATTGTTTGGGTTCAATTGATTAGAATGATTGTTTAAATTTGATCTGTATGCTGAATTATTAGAATTATTTTGATTTGAATAATTATTCATTTGTTGTCTTGTATGTGTGTTTCCACTTACTTTATTATTTTTGTCATTCATAATTTACTCCTAATTTTTATATTCATTCGGCAATCCATAATGATCACAAAGCACAATGTATGCCGCCATTTTTTTAGCAAGTTTTTTTGATGTTCCATATCCTTCTGTGTATTTAGTCCATAAGCCCATTCATTGAAAATTTCTAAAATTAACATTACATCTGCAATGTCATCATTGTTTGCCGTTATTATCAATTATGGGTTTTTCTGATATTTGATATATTGGCATTGAGCATTCACCACGCTCTGCTAATTCTTTTAATGTATTGATAGCATTTTCTAATTTAAATTTATCCGGTCTTTTTTCTCTGCAATCAACATCTTTAAAAAAATGTTCAATATCTAACATCTTTATAATGATGTTTTTTAGACGCTTAATATCTCAACCACTGTCAATTGCTATTGCACCAATTATAGCTTCAAATAAATCAGCTTTCACACTATCTTGATTAGCAACATTATTTCTTTTATCATTATCTCCTAAAACTAAATACTTAGCAAAACCTAATTTATTAATTTTTTTAGCTAGTGTTTTATTGCTTACGATTTGATTTTTTAAATCAGTAAAATCGGATTCATTTTTATTGGCTATAAGGCAATATTCTTCAAGATCTTCATATTTGTTGTAATATTGCGAAGCCGATTTTAAAAAGCCAAATTCTTCTGCCATTAGTTGAACTACGCAAAAGCTCAAAACTGAATCTCCTATAAATTCAAGAACTTCATTATTTTCAACTCCATATTGATTTGAATAAGAACTTCTAGTAAATGCTTGATAAAGTAAAAATTTATTTTTGAAGAAATATCCTATTGTATATTCAATTTGATAAATATATTCTTGCCATTCTTCTATTCCCATATTTGAATTTTTCCTTTCTAAAATAATTATTGTTCAATAGTTAAAATATGCTTTTGTTATATATTTTAAATTTTATTTAATTAAGGCAAAATAATTTTTATAATATAGTCCATCTAAACTTCTTTATTTTTAAGTGGCAAAAAAGTTTAAATAATACTTATTATTCAACTACTTTAACAAAACTAAATTATTGAACAATTTAATTATACTTTATATTAAATTATGACTTTATTTCATTCAAGAATAAATTTAAATGTATGTATATTAATTTTGCTATTTTGGTTTTTGCAAAATAGAATTACAATATGAGTTTTATTTGATTTTAGCGTTTTATAATAATTTAAATAATATAATATTTATGTATTTAGGAGAAATTATGTCAAACGAAGAAAAAATTTATGTTTTTAGTTATGGAACTATTCAAGATCCACAATTTTATAAGGAATTATTACCAAACTCAAAGCCAATGCCTGCAATATTAAACGGTTATGCAAAATGTGTTGATGAAACAATGTATTTTTTATTAAAAAAAGATTTGTCAAGTCAAGTTAAGGGAAGCGTATTTGAAATTAATAAAGAAGAATTGTTTTTAATTGATAGATGAGAATTATTTCCTCAATATCAAAGATTTCAAGTCAATGTTTTATTAACCGAAACTAATGAAATTTTAGAAAATGTTTATGTATATACAAAATTAGAAGTCGGAAAATATTATTTAGCAACAGATGATATGGGATTTTCTAGAAATCCTAATGCCAATGAAAATAATTTAAATGCTTTTATCGAAATGGAAAAGGCAATAAAAGATTTTCCATTAACTGACTATATATTTTTATATGATATAAACGAACAAGAATTTGAAGAAATTAATAAATTAACTCACCCTTATGCTGCTTTAATTATTGATGATAAGGAAAATAGAAATTATGTTGCAATTCACGGATCAATTTTTGCAATTAAAGAAGACGGTAAAATGTATGCAGCCTTAACTTCGTTTAGCCAAAAATCAAATTTAAATAGTATTTTTTATTATCAAGCATTTAATGAAAAGTTATTAAATAGCAAACCAGAAATAACGTTAAAATCGTTATATGATAATACAAATATAGATTTTTTAATTAATAAAAAACCTGTTTATTATTTAAGCTCAAGAGAAGACAAAACAATCAATGAAACTCAAGTTGGTTGATACGAAAATAAAGCCTTTGAATTAGTTGAAAAAGATTTTGACATTGATCCATTTATTCGCTTTAATAAAATGTTAAAAGCATTTTTTGATACAAAACAAAAAAATGATAAATAAAGAAATAATTATAAAAAATGTGCCACATAAACCCGGAGTTTATTTATGAAAAAATAAAAATGGCAATATTATTTATGTTGGCAAAGCAAAAGATTTGAAAAATAGAATGTTGCAATACTTTGATATAAAAATGCAAAATTCTTTTAAAACAGAAAAAATGAGGCAAGAGATTGCCGATTTTTCTACAATCATTTTAAGCTCGGAACGTGAAGCATTTATTGAAGAAAGAAAATTGATTGATTTTTACAAACCATTTTACAATGTTCTTTTTCCAACTAGAGATAGTTTCCCTTATATAAGAGTTAAATTACTAAGTACTAATAAATTAATAATTGATATAAAAAATCATTATAAAAAAGAACCAAATTCTATATATTATGGTCCTATTCCCGACAACAAAAATTTCAAACCATTAATTAGATATTTGAATCATTTACTACTTTCTAAGGATGGAATCATAATTGAAAAACAAAGTCTTGAATTTGCAAAGCAAAGATTTGAACAAGCCAAATCAATCATGAAATTTGGTCCTGATTTTAAAAGAAATTTAAAACAAAAAATCAATGAGGCAATTGAGGCTAACTTGTTTGAACAGGCAAGATTTTATGACCAAATTTTAGATTTATTAAATTACAATAAACAAAGTCAAAATATTTTTATAAAAACTATCAAAGATATTGATGTCTTTGGTTTTTATCAACATCAAAACTATATGATGGTTAATGTTATGATTTATAAAAACGGATCATTAGTAAATTCTTACGACTATATTTTTGAAATAATATTGAATGAACATGAATTTGTTAATCAATTTATTGATGAATTTTATGTTAATAATCAATTACCGGATCAAATAATAATACCTTTAAAATATTATGAATTAGGTGGATTTGATTATAATGACAAAGTAAGCGCGCCATCGAATAAATTAAATAAAAACTTGATCGATATTGCTATTGAGAATGCATCAAATAATATTGAACAAAAAATAAAAAATTATTTGAGTAAGCAAACAAAAGACAATGAAATTTTCAATAAATTATGTTCATATTTGAATGTTTATGCAGAGAGAATTGTTTTATTTGACAATTCATTTTTATCAAATACTAACGAAGTAATAGGGGCGGCTTGCTATTTTAAAAATGGCCAAGCTTTTAAAAAATTCTACCGTTTCTATAATTTAAAATTGAATTTATTAAGAAATGCAGATGTTGAATATATGCAACAAACTTCCTTTAATTATTTAAAAGATTTGGGAGATCAAGTTGATGTTGTTATTGTTGATGGTTCAATTGGTCAAATAAATGAAGTTAAAAAATCAATGGCATTTTTAAATTTAAATATTCCTCTATTCGGGTTGGTTAAAAATAGTAAACATGAAACTCAAAAATTAATTGATTCAAACAACAACGAAATTAAAATAGATGACCAAGATGTATTTAATTTGCTTTCAAGAATGCAAACAGAGGTTGATAGGTTTGCAAAATATAGATATAACCAAAAACAAAGAAAATCAATGTTATCTAATCCTCTATTAAAAATAAAAGGTATTGGATTTAAGACAATAGAAAAATTAATTAATTATTTTGGCAGTTATAAAAAAATTCAGCAAGCTTCTTTTGATGAAATATCAAAGGTGGCAAACAAAAAAGTTGCTAAGCTAATTTGCGAAGATAAAGGCGAATCGTTTTATTAAATTATTTTTCAAAAATATTAAAAAAATTTTTAAATGGTGTAAAATAATAAAGCAACAAAATAATGGTCGTGTAGCTCAGGGGATAGAGTACGTGCCTTCTAAGCATGTGGTCGCAGGTTCGAATCCTGCCACGATCGCCATTTTTTTTATTCATTTTTTTAAAAAAATAAAAAATATGTATATAATTATTAACGCATACAATTAAATACGCGAGTGTAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGAATACGTGGGTCCGATTCCCATCACTTGCACCATTGCGGATGTAGTTCAATGGTAGAACATCAGGTTGCCAATCTGAATACGTGGGTCCGATTCCCATCATCCGCACCATTTGAGTAAAGTTCACCAAAATTGACATAGGCTAAGGCTTGTGTCAATTTTTATTTATAAAATCGAGCGAATACTGTTACTGCTATATTTCATAAAATCTTAAATCTGGCAGTTATTATGGTTAATAAACATCGGCTCCTTTTTTTATTATTTTCTTTATTTTTTATTAAATAATAAGGTGCAATAAAAAATTCAAATTAAAAATTAAAGTTTGAAATATGAATAAAAAAAGTGTGCAAAGACGTACTAAAACCATTATGATATTATTTTTATTTTATATTGAAATAAGCATTTAATTACGAGTTTAAATTTTTGTCCTATTTTAAGGCTATACATCTTTTTTGTTTTTAAAAAATATTGATGTTTAATTTATTATAATTAACTTAATATTTAGCAAATACATTAATTGACTAAATATTAACTTTAAAAATATAAAGGAAAATTATGGCAAAAGTATTAATAATATATGGTTCAGTTATACCAAGAGAAAATTCAATTTCAACAAAAATGTTAGATCTTTTTGTTGATGAATATAAAAGAAATAATATTAATGATGAAATAAAAATTCTTGATTTAAATAATGAAGATATGTATTCATTAACTGACAAGAATTTTAAATTGTTTTATAGTGAAGAAAACTCTGACAAATATATAAATTTATTGAAGTCTGTTGATAAAGTTGTTATGAATGCGCCGATGATAAACTTCAATATTCCAACTATAATCAAGGCATTTTTTGACCGTATTGCAGTTGCAAATAAAACATTTTCATATAAATACGCAAGCAAAGGTTCTTCAATTGGTTTATTGAATAATTTAAAAGTTCAAATAATTGCAACGCAAGGAGCTCCGTTAGGTTGATATCCATGAGCAAGTCATATTAATTATTTTGAAGGAATGTGAAAGTTTTTAGGTGCAAATCTTTCAGAAAGCATTTTATTAGCTGGCACAAAAGTTGAACCTTTAAATAAAATGCAACCTCAAGAAATAATTGAAACTATTAGCAAAGAAATTATTAAAAAAGCAAGCATATTTTAATTATTTTTTGTTATAATGTAAATGCAATTTTTATTGCCACATTAAAGTGGAAGAATTTGAAACAAATTCGTTTGAACCACTATAATCGAAAGGATTTTATTTATGGCTAAAAAAGTAGTCAAGAAAGCAAAATTACAATTTGCTGCCGGCCAAGCAAAACCAGGCCCAGCATTGGCTGGTGTTGGTATTAACATGCCAGAATTTACAAAGGCATTTAATGACAAAACAAGAGAAAGAGGAAATGAACCAGTTCCTGTTTTAATTACTGTTTATGAAGATAAATCATTTGAATTTAAATTATTTACTTCTCCAACATCATACAAGTTAATCGAAGCTGCCAAAATTAAAAAAGGTAGCGGAGTTGCAAACAAAGAAAAAGTTGGTTCTATAACAATTGATCAATTAAGAGCAATTGCAGAATACAAATTACCTGATTTAAATACAACTAAAATTGATTCAGCAATGAAACAAGTAGCTGGTACTGCAAAGAATATGGGAATTACAGTTGAAGGTTATGACGAATGACTAAAAGGAAGTGCTAACTAATGGCTAAAAGAATTACAAAAAACATTAAAAATGTTAAAAGCTTAGTTAATAAAAAAGACGTATTCCCTCTTGCAGATGCTATTGAATTAGCAAAAAAAGCTTCATTTGCAAAATTTGATGAATCTGTTGATATTGCAATTAGATTAAATCTTGATACAAGAAAATCAGATCAACAATTACGTGGATCAGTTGTACTTCCACACGGTACAGGAAAATCAGTTAAAGTTTTAGTAGCAACTGACGAAGTTTCAGCCCAAAAAGCTTCATTAGAAGCTGGAGCAGATTTTGTTTACAGTTCAGCTGAATTACCAGAAGTATTAAATCAAGATAAATACGATTTTGATGTAATAGTTGCCGATCCTAAAATGATGTTAGTTTTAGGAAAATTTGGTAAAAAATTAGGACCTAAAGGTTTAATGCCAAACCCAAAAACAGGAACAGTTACAATCAATCCTGCTAAAGCTGTTGAAGAACTTAAAAAAGGTAAAGCAAATTATCGTGCTGATAAGGGTGGAATTGTTCACGCCTCATTTGGTAAGAAATCAATGGAAACAAATGTTCTAGCAGAAAATGCTGATACTATTATTCAAACAATTAAGAGATTAAAACCTCAAACAGTTAAAGGTACATATGTTTTAAATATTACCGTTTCAACTTCAATGGGTCAATCTGTTAAAGTAAAAATTGATTAATATGTTGAAAAAAAGCAGTCTTAACACTGCTTTTTTATTTGTTTTTAATAAAAAAACAAAATTGTTAAAATAAAAAATACCTCAAAATTAGAGGTATTTTTTAAATTTAGTTAATTTTTTAGATTATAAAATGAGTTTTTGCCTTCGAATTTAGCTTCATTGCCTAATTCTGATTCTATTTTTAACAAGCGATTGTATTTAGCAATTCTATCTGTTCTTGAAAGAGAGCCGGTTTTGATTTCTCCAGTATTTAATGCTACTGCCAAATCTGCAATAAATGTATCTTCAGTTTCACCTGAACGGTGAGAAATTATTGCTACAAAGCCAGCTTTTTGTGACATTGTTATTGCCTTAAATGTTTCAGTTAATGAACCTATTTGATTTATCTTTATTAAAGATGCATTGATTGCATGCTTGTCAATAGCCATTTGAATGTATTTTGGATTTGTTACAATTAAGTCATCTCCAACTAGTTGAACTTTGTTGCCAAATCTCTTATTCATTTTTATAAAGCCATCTCAATCGTTTTCATCATGTGAATCTTCAATAGAAATTATTGGGTATTTTTCAATTAAATGGCCATAATATTCAACAAATTCATCGCTAGTAAATGAATATTTAACATTTTGATATTTTTCAAATCCTGGGCGTTTTTCTTCAATTGCTTTCTTGAATTTTTTAAATACATATAACTTTGTATCTGAATGATAAAGTTCTGAACTTGCAGCATCCAAACAAATTGCTACTGCTTTTTCTCCTGAAGTGGCTGGATTATATCCTGCGACTTTAATTGCATTTACTAAAAAGTCCAATGCTTCTTCATGAGTGTGTAGGTTTGGTGCAAATCCACCTTCATCTCCAACTTGTGTTCCATGACCTGCTTGTTTCAATAATTTTGCTAATGTATGAAATACAAAGTTTGCCATTTGCAATGATTCTTTAAATGTTTTTGCCCCAATAGGCATTATCATAAATTCTTGGAAGTCAATAGTATTTGAAGCATGTTCTCCACCATTAATAACGTTTAGCATTGGCACTGGCATTTTGTATGCTACTTGGTTGTTTAATTTTGCTAAATATTTATATAATGGAAGATTTGCTTCAATGGCACCAGCTTTTGCAATTGCTAATGAAACAGCTAATATTGCATTTGCCCCTAATTTTGATTTTGTTTCAGTTCCATCAAGATTAATCATTAAATTATCTAATTCTTTTTGTTTAGAAACTTCCTTGCCAATAATAGCTTTAGCAATAATATTATTAACATTATCGCATGCTAATTGCACACCTTTACCGCCAAATCAATTATTTTCGTATTTTGTTCCTTTGTCGCGTAATTCAAGTGCTTCTTTTGATCCTGTTGAAGCTCCTGATGGTACTAATGCTTCAGAATATGCAAGTTCAGTATTTAATTCAACTTTAACTGTTGGATTTCCTCTACTATCTAAAACTTCATAGGCATTAATCTTAATAATTTTTGACATAATTCTCCTATATGATTATTTAAAATAAAAGCTAAAAATAAATGAACATCATTTATGTTAGTTTACATAACAATTATATTATTAATTTTTAAAGGTAAAAAAATAATTGTTATTTTGTGTGTTCAATCTAAAAGAAACTTAATTTTAAAAATATTTTTATCTAATGAAAAATTGCAATTTTAAATTAGTGTAAAATTTTATTTATGAATAACATTGATAATAATTTATTTTTTATTGAAGAAGAAATTAAAAAAATTGAAAAAATTGCAAAAGAAGACCCACTTAAAGCAATTTATGTTTTAGATGAAGACAAAAAACAATTCTCTCTAAAAGAAGACTTGGATGATTTAGATGCATTGAGGGATAACATTGAGTTTCAACTTAAGAAACAAAATTTAATAACCTCAACAAAACTTGATACATTAAGTTTAATAAATAGTTTAAAAAACAAAAAAATGGGTTATGCATTTATGCTGAGCTACAACGAACTTAAAAAACGAGATGATATAGCAAAATATGCAATTGAATTTCAAGATTTTTTTAAAGGAAATGATTTTGATAGTAAAGGATTTCAAACATTGATTTATGACTTATTAGTCAATAGTAATATTGATTTTGAATACGATATTCAAAATAAAAAAATTAATCCCAAAAAATTAGGATCATTATTAAAGAATGCTGAAATTCAAAAGATGCAAGATGAAATTATTGCAACTTTTGACAAAGATATTTCAAAAAACAAGGTTGCAAGGCAAGTGTTTTCAGCGTATTTATTTCAAAATTGAGTTGCAATTTTATTGCACGAAACAAAAGACGATTATTTAAAAATATCACACGTAATTGAAGTAATTTTAGGTAATCAAACGTCAGATTCACTTTCCAAAGAAGAAAAAGAGTTATATAACATTTTTAAATAAATTATTATGTATAATTTATAACATTAAACTAAAGGAGCATTTATGTCAAGAACATTAAATAAAAAAGAATCAGAATTAACTATTGAATATACAATTCAAGGTGAACAATGAGAAAATGCTAAAGAAAAAGCAAGAAAAGCATTAAGAGCCAATGTTCAAATCGATGGTTTTAGAAAAGGCCACGTTCCCGCTTTTGAAGCTGATAAAAGAATAAATCCAATTGAAGTTTATCAAAAAGCATTGGATAGTATTTTAGATAATATTTATAAAGAACAAATAATTAGCCAAATAACCAAAGATGATGAAATTGTTGGACATGCAAATTTAAGAATTAAAGATATAAATGAAGAAAAAGCAGAAATTTCATTTGTATTTCCACTTTTCCCAGAAGTTAAATTGGGAGATTATAAAAAATTGGGTGTTAAATTAGGATCATGTGAATTAACTAAAAAAGATTTAAATGATGCAAAATCACAAATAGCTTCAAACTATGTAGTTATGCTAGATTCTAAAGAACCAATTAAAGAAAAAGATCAAGTAAACTTTAATTTCAAAGGCTTTATTGACGGAAAACCATTTGACGGCGGAGAAGCTGAAAACTTTGATTTAGTAATTGGATCTAAACAATTTATTCCAGGCTTTGAAGACAAAATGATTGGCCTAAAAGCAGGAGAAGAAAAAGATTTAGATTTAGTATTTCCAGAAGATTATCATGCTAAGAATTTAGCAGGAAAATCTGTTGTTTTCCATGTAAAAATAAATTTTGTTAAAACTCCAAATTATCCAACAATCGATGATAATTTTGTAAAAGAAATTAATTTGCCAAATGTTAAAAATGTTGCTGAATTTGAAGAATATGTAAAATTTATTGCTTTAAAGAACAAATTAGCAAATGTTAAAAATGAATTTATTAATGAAGCAGTTAAAAAATTATCAGAACAATCAGAAGTTTCTTTATCAGAATCATTAGTTGAAGAAGAAGCAGATAAATACTATCAAAATTTCCTTAACAATTTAAAACAACAACAAATTACTGAAAAAGATTATTACGAATTTTCAAAGAGTACAAAAGAAGAATCATTAAATGTATTTAAAAAACAAGCCCAAATAAACTTAAAACAAATGTTTGTATTAGGTGCTATTGCCAAAGCTGAATCTTTAAAAGTAACATTAGAAGATTATGAAAAAGAAGTTGAAAAATTGGCAAAAATATACGGCTTAGCTCCTGAACATGTAAAACAAATTTTGAAATTTGAAAATGTTGAAAATAATTTAATTAATGAAAGAATTAGTGAAATTTTACTAAAAGAAAATGACACTAAAGCATTTGAAAACTTTGACAAACTATTAAAAGAAGTCGAAGAATATGAAGCTAAACAAACTCAAGCTATTGTAGACGAAGCTAAACGCAAAAGAGAAGAAGCAGAAAAAATAGCTAAAGAATCAAAAGAAAACATTGACGAACAAAAATAATATAATTTAAAAAGTGCCATAAAAATTGGCATTTTTTATTTATTATTTTTAACAATCATTAAAAATAAAATAATATTGTAAAATATTAACTATGAAAATATTTGAATTTAAATCAACTGAAATGAGAATTGCAGAGGCAACCCTTAAGAGGATAAATGCCCTAGAACCAGAAATATCAAAATTAAGCAACGAGGAATTGCAACAAAAAACTATTGAGTTTAAGTCGCGTTTAAAAGCAGGAGAAACGCCAGAACAAATTAGACCTGAAACGTTTGCTGTTTGCAGAGAAGCAACAAAACGTATTTTAGGCAAAAGACCATTTGATGTTCAAATGATAGGCGGAATAATTTTAGACCTAGGTTCTGTAGCGGAAATGAAAACCGGTGAAGGTAAAACTATTACTTCTATAGCACCAATTTATTTAAACGCAATTACTGGTCAATCTGTTATTGTTTCAACAGTTAATGAATATCTAGCAGAACGTGATGCTCAAGAAATGGGGCAAGTTTTTAACTTTTTAGGTTTGAGTGTTGGAATAAACAAATCTCAAATGTCAAGTGAGTTAAAAAGAGAAGCATATGCTTGCGATATTGTTTATTCTGTTCATTCTGAATTAGGTTTTGATTATTTACGTGACAATATGGCAATGAGTAAAGAAGAAAAAGTTCAACGCGGGCTTGATTTTATTTTACTAGATGAAGTAGATTCAATATTGATTGATGAAGCAAAAACTCCTTTAATTATTTCAGGTGGCGATAATGACGAAAGCAATTTGTATACAATTGCAGATTTGTTTGTAAGAACATTAAGTCAAGATGATTATTTTATTGATGAAGAAACAAAATCTGTTTACCTAACAGATAAAGGAATTGAAAAGGCTAATAAATATTTCAATTTTAAAAATCTTTATGATATAGAAAATTCTGAACTTGTTCATAGAATTCAAAATGCTTTAAGAGCACATAAAGTAATGAAATTAGATGTTGAATATATTGTTAGAAACGACAAAATTGAATTAGTTGATTCCTTCACAGGTAGAATAATGGAAGGAAGAGCTTATTCAGAAGGATTGCAACAAGCAATTCAAGCAAAAGAACGCGTTGAAATTGAATCAGAAACCAAAACATTAGCAACAATTACTTATCAAAATTTTTTCCGTCTATTTAAAAAAATAAGTGGTATGACAGGAACAGCAAAAACAGAAGAAAAAGAATTTATTGATATTTATAATATGAGGGTCAATGTTGTTCCAACAAATAAACCAATAGCTAGATTTGATGATAAAGATGAAATCTATGTTGATATGCACAGTAAATGAAAGGCAGTTACTGCAGAGGTTAAACGTGTTTATAAAAAGAAACAACCAATATTAATAGGAACTGCTCAAGTTGAAGATTCTGAAATATTACATGAATATTTGCTATCTGAAGGCATACCTCATACAGTTTTAAATGCAAAGCAAGATGCTTCAGAAGCAGAAATTATTTCTAAAGCGGGACAAGTAGGAGCAGTTACAATTGCCACAAACATGGCTGGACGTGGAACAGATATCAAGCCTTCAAAAGAAGCACTAGAATTAGGCGGATTGTATGTTTTAGGAACTGAAAAAGCTGAATCAAGAAGAATTGATAATCAACTAAAAGGACGTTCTGGCCGTCAAGGAGATGTAGGATATACAAAATTCTTTTTATCATTAGATGACCAATTAATTTTGCGTTTTTCAGTTCAAGATCACTGAAAAGAAATTTTCAAAGAATATGGCGAAGATCCAATTCCTGGTGAAGCAATTAGAAAGGCCTTTTTGAGGGCTCAAAAAAAGATTGAAGGGTTTAACTTTGATAATAGAAAAAGCGTCCTAAATTTCGATGACGTAATTAGACAACAACGTGATTTAATTTATGAACAACGTGATCTAATTTTAAATAGAGATGATCTTGGAACAATAATTAGAAAAATGATATCAGTTGCAGCAGAAAAAACAGTAAATAACCCATTTTTTATAAAAAACAATAGTTTAGATTTAACAGAATTTGTAAAGCATCTAAATAACAATTTTATGATCTTAACAAACAAAGAATTTAGTGTTGAAGAATTAGTTGAATATGATCGCGAAGAATTAATTCAATACTTAATTGAAACTTGAAATAAATGTTATGACCAACTAAGACAAAATATCATTGATAAATATGGGATATCCTCATTAATAAATTCTGAAAGAAATATAATATTAAGCGTTTTTGATGCTGCGTGACAAGATCATATTAATATAATGGACAGATTAAGAAGAAGTACAAATTTAGTTCAATATTCTCAAAAGAACCCATATCAAGTTTACACTCAATTGGGTTCAAAGAAATTCAAAGAATTAACAGACAGAATTGCTTTGGAATGCACAATTAATTTAATGAATAATTACGACGCAATACCTTCAACAAATACTGATGTTGATTTATCATGGTTAACAAATGCTTTTGAAGAAAAAACAGATATGGCTCAAACAACTTCAAAGAACCAGCAAGCTCTTGAATTTATCAATTATTTATTGAAGTCAGAACGTGATCATTTGATTAAAGAAGGTTTAAACGAACAAGAAGTGGATGATAAACTAAAAGAAGTGGAAAGATCAATATTAAAAGAGTTCCAATTCCAACTTGCTTCTTTGCAAACTAATGAAGAAAAAAAATAACGAGTTGTTTAAAACCCGTTATTTTTTGTTATATATTCGCAATAAAATTCAACAGCCTTTTTCAATATTTCACAATCACTTTCGTATGTTATGCGATATAACAATGCATTTGTTGTATCTTGCCATTCAACTACCTTAATTTCACTATCTTGCTTAATTAAATCAAAAGATGTTGGCTTAGCAATAAAATAAACAACTTCTTTAGATATTAATTTTCCAATAATATATTTATTGACTTCTCTAAAACCCTTGAGCAATTTTATATCTATGTTAGTTTCTTCTTTTACTTCTCTAATCGCGGTTTCCTCTTCTGTTTCGTTTTTTTCAACATGGCCTTTTGGGAACCCTCAATGGCCTGCTGTTTGTTTAACTAATAAAACAAAAAGATTATCATTTTCTTCTTTGAAAATAATAGCGCCACACGACTTCTCTTTTTTCATTAGCGAAATTTCCCTTACCTTTCTTGACTACAATTTATTTATATAAATTATATAACAATAAAGAAAATGAGCGCTTTGCCAAAAACTTAATTATTCATCTTTTTAAAAATAAAACTACATAATATTAATGATGAAGCAATTGCAACTAAAAAAATCGTACTTGAAATTCACATTTCAACTTTAATTACTTTGCCTTGTCAAAGTATACTTTGTAATAAATTATTATTTTTAGTCATTATTGCTTGAATGTATGTTTTTCTAATTGAAGGTAAAATCAAACCATTAACAATAAAACAAATTCCACATATCAAAATAAAAATGCTGATTATTAATAGAGTTTTTAATATCTTTTTGTTTGCAATTTCTGATTTAAATCTATATACTAAAAAGAAATTCAAAATATAATAAACAACATAAAAACCGCAAATTATTTGTCCAAAGCAGGATATTGCAAGACGGCTATTATTTGAAAGTGATTGTATTGAAAATATCCCAGTAATCAAAAAAGAAATCAAAATCGCTAAAAAGCCAAATGCGGTTGTTATTAATAATCTTTTTATATTATTTTTTATAATATTGTTAATATCCATAATGCCTCTTTTGAAATATTATAAATGATAATAATAACCTTATTTTTGAATTTGGTAAAATTAAATTATTAAAAGGAGAAAATGTGGCACTTACTAATAAATATTTAGCATTGTATAGAAAATATAGACCAAAAATTTTTGATGATATAAAAGGCCAAGATCACATTGTAACAACATTGAAAAATATTATTTTGAATAATAAATTGAGTCATGCGTATTTATTTTGTGGCCCCCATGGAAATGGAAAAACTTCAACTGCTAAAGTTTTTGCAAACACAATTAATTGTTTACATAATCAAAGCGATATATTAAAACCATGTGAAGAATGTATCAAAAATGTCGATAGAAATCTAGATATTATAGAAATAGATGCTGCTTCTAATACCGGCATTGATGACATTAGAGAATTAAAAGAAAAAATAAAACATTTACCAACACAAAGCAAATATAAAATTTATATTATTGATGAAGTTCATATGCTTTCAAAAGCAGCCTTTAACGCTTTATTAAAAACAATTGAAGATCCACCTAGTCACATAATTTTTATTCTAGCAACAACTGATCCCCAAAAAATACCAGAAACTATTTTAAGTAGGGTTCAAAGATTCAATTTTAAACTTATGACAATTGAAACATTAGTTTCTCAATTGAAGGATATTTTCAATAAAGAAAATATTGAATATGAAGATGATGCAATTAATGTTATAGCTAAATTAGGAAATGGTAGCCTTAGAGATTCATTAAGTATTGCAGACCATGTTGCAATATATTGTGCCAATAAAGTTATTAAGAAAGAAGTTGTTGAACAATTATTTGGAATTGTAAATATAGATAATAAAATAGAGTTATTAAATTGTATGTCAATGCATGATATCAATAAAACATTGAATCTTGCAAGAGAATTATTTAGTCAAGGTATTGATATAAACATCTTGATAAACGATTTAATAAACATTTTAAAAGAATGAATAATTTTTAATAAAACAAGAGATCAAAGTCTTGTTTCAACTGATTTTAAAATTTTATTGCAAATTAAAATTGATGATGCTAAAGCACACTATTACATTGAAACATTGATAGATCAATTGCATAGTATTGCCGTAAGTGATAGTCCAGAAGATTATTTCGAAATTCTGTTGCTAAAGTTATGTAATTACTTAAGTCCAAAAGAAGAATACAAAAATTCTGCAGTAATGCCAAAAATTAGCAATGAAGAATTTAATAATTTTAATAGTCAAGAAAAGCAGGCCATTAATAAAAATAATATTTACAAATCATTGGATTTATCCTCAATAGCAAATGGATTCAATGCAAACAATTCCACAATACAAAATTCAAGTGATAATAATTCAACAGAAGACTTAGCTAATAATTCAAAAAAGATTGATGACTTATTAGAAAAAACTGCAGAAATATATTCATTAGATCAAACATCAGAGTTTGAAGTTGCTGATATTTCTAATCAAATAATAAGTAATAATCAAATTAGTATAGATTCAAATGTTGATAACCAAGAAACAGCCGATTTACAAATATCGGAAAATTGAAAAGATTTAGTAAATGAGGATTTAATTTCAAAAGAAAATTCAGAAAATAAACATGTTCCTTTGGAAACAATAATCGACTGTTTATTAATAAAACAATATTTGAAAAATAATATGTTAAATGATGGTCGTAGTAATTTTACAGACATTGATGCTATTAAATATGGTATGTTAAGTCAAAAAATAACAGACAAAAATGAAATTAACATCTTATCAGACTTTAAAATTATTTTTTCAACGAATGATTTTATTTTGTTAACATGTGATATAGATGAAAAAGTCTACGATTTAAATATAAATTCACATAATGAAAATATTATGGCTGCATTGAATAAAGTTTTTGATAGATATCTTTATGCTTATGCCATTACAAAAGGCGAGATATTAGAAGCAAAGAAATATTGGAAAGAAAATATAAACGAAATAAGAGCCAAAAAAGTTAAACCTTTGGAAGATTTAAATTTAAAATATAATAAAACAGTTCAAAAAGATGTTGCTTGAGCACAAGAAATTTTTGGCGATAAGTTCAAGTTAAACAAATAAAAATTAAATAAGGAGAGAGTAGTATGAGTATGAACATGAATGAAATGCTTAAAAAAGCACGTAGAATTCAAACTGAAATGGAAAAAGATGAACAAGAAATTCAAAAGAAGGAATTTACTATTGAAAAGCAAGGCATAACCGTTGTTATGAATGGTTTAAGAAAATTAGTGTCTATAAAATTTAATCCAGCATTAATCGATCCCGATGACCCTGAATTATTAGAAGATTTATTGATGCTTGCGGTTAATGAAGCAATAGAAACAGTTGATAAGGCTTTTGATGAAATTAATGCTAAATATTCTGATAGTGGCCTTTCATTCTAATGAGCAATATTGAAATACAAAAACTAGAAGAACTTTTAAAAACGGTTTTTGATTTTACTAAAAAACAATCTCAAAAATTTATATTCAACATTTTTAAATCATCAATTGAAGATATAGACCAAGTTTGCAATCAAATAAAAGCATTGAGAAATTCAATAAAAAAGTGCAACATTTGTAATAGATATAACGAAAATACAATTTGCAATATTTGCTTGGATACTAATCGAACAAAACAACTTATGTTAGTTGGTAATTCGTTAGATATTGATAAATTTGAAAATATTGGTTTGTATAAAGGAAAATATTTTGTATTTGATCAAAATATAAATTCAAAGAATGAAGATTCTATAGTTTCAAGTTTATTAGAAAAATTAATAGATATATTAAAAGATAAGAATGAACTAATTCTTTCACTTTCCCCAACTATTGAGGGTCAATTTAATATGCATTATATTAAAAAGTTCTTGAAAAACAAAATAAATTTTACAAATATTTTTCAATTATCAACAGGTATCCCTTTAGGTTCATCAGTTGAATATATTGATCAAGATACATTAAAAGAATCATTATTGAATAAAATTAAAATATAGTAGGAGCATTTATGTCAAAAGGAAAATTTGTAGTATTTGAAGGGATGGATGGTTCAGGAAAAACAACTATTATAAAGAGATTAAAAGAATATTTAGAGTCTAAAAATAGACTAGATGATTTCGTATTTACAAGAGAACCAGGAAGCTCACATTCAAAAGAAGCAGAAGAAATAAGACAGCTAATTTTAGATAATGATAATTCTTTTAGTGTTATGGTGGATGCTTTGTTATTTGCAGCAAGTAGAAGGTTAAATCTTGAAAAATGCGTTTGGCCAGGATTGGAAAAGAATAAAACGGTTATTTCAGATAGGTATTGAACCTCATCATTTGTTTATCAAGGAGTTTTAGGCGGTTTAGGACTTAAGAAAGTAAAAGACATCAACAATATTGCAACTGAAGAAACAACACCTGATTTAACTATATTCTTTGATATGGAGCCAGAACTATCAGTTGAAAGAATTACGCAAATTCGAAAAACAAAAACTGATAGATTAGAATCTACCAATGTTGAATATTACAAAAAACTAAGAGATTCTTATTGATATGTTATTAATCAAGGTAATGAAAAATATGAAATTATAAAATCTGATTGTAATATTGATGAATTATTTAAAAGAGTATTAAATGTATTAAAAGAAAATAAAATAATTTAATTTGATTTTGTCTTTTTATATTTAAGGTTGTAAAAATATGATAAACAAAAATTTGAGTAAAGTATTGGAAAATTCAATAAAAAACAATAAACTTCAACAAGTTTATTTACTATTTTCACGTGCAAATCAAGATATTGATTTAGATATTTTAGAATTTGTTAGATTAGTTAATGATGAAAAACAAATAAGCTGACAACAATGTTTACAAAAAGAATATTGTTTTATTATTGATGGAAGCATAGATGAAATATCAAAAGAAAATTTACAAGATTTTTTAAATCGTGTTTCAAATAGTTCATTATTTGCTAATAAGTATAAATTTTTAATTATTAAGTGTGTTGAAAATATTACACTAAAGGCCGCAAATAGTATTTTAAAATTTTTAGAAAATCCCCCATTTAATACAATAATATTATTGCATACAATAAAGCCCCAAAACGTTTTGAAAACTATCAAATCAAGATGTTTTATTATTGATATTCGTGATGATTTTAATGCTGTAGCTAGCGTTGAAAATTTTTTTATTACATATAAAATTGACGTAAGCAAGCAAAAACAATTTAGGGAACTTGCTGATTTAATATACAATTCACCAGCAAATCTATTTAATCTTATTTTGTTTTATATTCAACAATTAAATATAGAAAATTATAAAAATATTATTCATTTTTCTATTTTTATTTTTAAACAAATTTACGAATTAAAAAATAGCATAAAAAAAGATAAAAATATTAGTAAATTTGATTCTATTTCAATATTAAATATTATTAATAATTTATATAATTTTGTTGAATTAATTGATAACAATAATTGTATTTTCAATATTCAAAAAGCAAGGCTAATTTTAAAATTAAAGGAAGAATATGAATAATAAAATTTATATAGTTGGAACTCCAATTGGAAATTTAGAAGATATAACATATAGAGCAATTAAGGTTTTACAATCCAGCGATATAATTTTGTGTGAAGATATAAGAGTTAGTCAAAAATTATTGCAACATTATGAAATTAATAACAAGCGCCTAATTGCATACCATAAATTTAATGAAAAAGAAATGACACCTAAAATTATTGAATTATTGCAACAAGGGATGATTATTTCATTGATTTCTGATGCCGGTATGCCTTGCATTTCTGATCCTGGTTTTGAAGTTATTAAAGCAGCCAAGGCAGCAAATATTGGCATTGATGTAATTGGCGGCCCAACCGCTCTAATACATGCAATAATAAAGGCTAATTTAGACAATTGCTTCACATTTCTAGGCTTTTTAAAAGATAAGTCAGGTGCAAGACAAAATCAATTAAAATCCTTGACATTTGGCACATATGTTGCTTACGTTTCACCTCATAAATTGCTTTCAACTATTAAAGATTTTGAGATTATTTTTCAAGAGCAAGTTAAATTATTTTTAATTAAAGAAATGACAAAAATGTTTGAAACCTCATTTGAAGGAACACCTCAAGAAGTTCTAACACAACTAAGCGAAGAAGATCAACGCGGTGAATATACTCTTGTATTTAGTTTCAAAGAACCAAAGAAACTTAAAATTAACAAATATAAAAAAGAAATAAAATAAAAAAAGTTCGGACGCGAACTTTTTATTATTTAATTTTTTAAATTTAGTTGTTATTTTTAGCAATAAATGCATCTAAACGGCTAGCTTTTCTAGAACCATTGTTTTGATGTAAAACGCCCTTTGAAACAGCTTTGTCAATTTCATGGTGAGCATTAGCTTGAAATTCAGCAGCTTTAGGATCTTTATTAACAGCAGCAATTTTTGCTTTCTTAATAGCTGTTTTAACTGATGATTTAACGGCTGAATTACGGATGTTTGCTTTTTCGTTAGATAATATTGCTTTTTGTTTTGATTTAATGTTTGCCATTTTTTGCCTTTCATGCTTAATAATTAGCAATAGTATTTTACAATAAAAAAGCGTTTTATTTAGAAAAATAATATTTTTATTAAAATATTCTATATGGTAACAAAGAAAAAATATGATGGATATCTTAAATATTCAAAAAATAATGAATCTGAGTTGATAAATTATTATCTAACTAATAATAAAAATATCTATTTAAAGTTAAACAATGAACAAGAAATCGTAGCTTATGTGCCACATAAGTATATGAATACAATAATATTGGATAATTTTTTAAAATCATCAGTTCCTAAACTTCTAGAAATAAAAACAAAAATTGAGCAAAAGAAAATACTTGCAATTGATATAGAAAAAAATAAATTTTATTTATGAGGCAAGGAATCAAAATTTTTAATTCAAAAAAATATGTTAAATTTATTTGAATTAAATAAGAATTTAAAAATCTTAAAACAAGGTATAGAATTTACTCTAAATAAATATTTAAAAAAAATATTACTGGAACTATCAACTTTATATGTAGAAAAATTTTGTAAATTATTATGCATACCATCCGAGTCAATAAAAGTAAGAATTTTGAAGAAGAATAATGCCTGGGGAACAAATCATTTTTTAAAAAGAACAATAACTTTTTCAGATCGGTTAATTTTTTACAGAAAAGAAGTTTTGGAATATGTTGCTTGTCATGAAGTTGTTCATTTTATTTATCAAAACCATTCATCTGAATTTTGAAATATGGTAAAAAAATATATTCCTAACTTTAAACAAATAAAAAAGGAATTAAAAGATAAAATTTTTATATAAGCAGGAGATTTCATGGAAAATAAAACAAAATTTATTAATGGAATTATTATAGGATTAGTTTTGCTATTAGTAATAGTTATAATTCTAATTTCAGTTTCAATTGTTCTTTCTAAAAAGAATAGAAGATTTTTAAAAGATCAAAAAGGATTTTTAGATTTATACAATACACAATTAAAAAATATTAGTGAATCTATAAAAAATTGCAAATTTATAGGTGCCAACGATTATTCTTATAACGATCAAAGAATACACTTAGATGATATATTTGTTACTGATAAAATTATTATTGTATTAAAGCCTTATATGTATGACTCTGACTCTTCTATTCAAGGTGACTATTTTAGCAACGAATGAATTTTAACTAAATCAAATGGTAAAAAATCTAAGATACCCAATCAATTAATTAGTTTAAAAAAATCTATGAATAATTTTGGACTATTGTTTTCTAATGAAATGAGTATATGTTCAATTTTTGTACATAATTTAAGCACGGAATTAAATATAGAAGATGTGCCATCGTTTCAAGGAATTTGCAATATTAAAGATTTATGTGCTTCAATAAAATCTGTATATAATTCGAATATTAATTCAATGTATAACCAATCTATTTCAACTATTTTCAATGTTTTAAATAATAAAATTGCAAAATAATTGTAAAAATTTAGTATTTTCAATAATTTTTCAATAAAACAACAATATCTTTTTGCTATTTTAGTGTTTTTGTGTAATAATTTAATTGTAAATATTAATATTTAATTTATTTATAAAATTTTTTAAAAAAATAACCTTACGTAAGGAGTATCTTAAATATGGCAAAAGAACAAAAAACAGTATTAGAAAAATACTATATTTCTGAAGCTTTTGATAAAGATAGAAATATTTCATTTAACTTTAAAAAGGCAAATAAGGGCATTTCAGGAAACTTTTTAAATTTTTCTAGCGCAGTTGAAGAATTTATTAAAGTTAGTGAAAATACACAAAACCCAACCAAATGTTGATTCCACCGTGATGGCGCTTACCGTGGATGCGTAAACATTGATGGTGCCCGTGTTATTGTTGAAAAAGTTGCTGAAAAACAAGTTAAAAATGAAGAAGCTATTGAATTCATCGAAAGAGAACAATTGGTTGAAAAACCTGCAGCAAAAGCTAAGAAAGTTGAAGAACCAAAACAAGAATGTGAATGCTCATGCTCTTGTGATTGTTTAACCGGTATTGAAAACAACAAACACTGAAAAGAATATTGAACAAAGAAATCTTCAAATAATATTGTTTGTTCAGCAACACAAGACCACAAAGGTGGAGAAAAATTATTTATTGGTCTAACAGTGTGTTTATCGCTTCTAACAATCATCAATTTAATCTTGATTATTTTGTTAGCAACAAATGTTATTTAATCCAATAAATTAAAAAATAAAAACTGGAATTCCAGTTTTTATTTTTTAATTTATTTTAAATTCTTTTTTAATTGTTTTAAGTAATTTACAAATTCTTCTTTGCTGTATGTTTTAGAATCATCTTTTCCATATTCTCTAACCGATAAGGTATTTGTTTCAATTTCTTTTTTTCCAATTACTACTATAAATTTGGTCTTTAAAATTTGAGCATCACGAATCTTTTTGTTTATTCTTTCTGGTCTTATATCAATATTAACATTAAAATCTTCATCTAAAAATTCGTTATATAGGTCTTGAGCTTTTTCATTTAATTCATCATTTATTGGAATAATTGTTATTTGTCTTGGACTTAATCAAAATGGCAATACACCTTTTGTTTGTTCAAGTAGTGTTGCAATAAATCTTTCATAAGTTCCAATTAAACCTCTATGAATTAATACTGGTGTTACAAATTGTTCATTACTATCAACAAATTTCATTTCAAATCTTGATGGTAATAAAAAGTCTAATTGTAAGGTTGACATAGTAATAATTCTATTTAGTGCAGTTTTTACTTGAAAATCAACCTTAGGTCCATAAAATGCTGCTTCACCAATAAATTCTTTATATTTAATATTTAATTCACCTAAAACTTCACGCAAGTCATTTTCTGCTTTATTTCACATTTCATCATTTTGGAAGAATTTTTGAGTATCTTGTGGATCTCTTAATGATAAAGAAATATGATCTATTTCAATATTAAAATCTTTTAATGCTTGCAATATCATTTTATACAAGTGCTTAAATTCTGATTTTATTTGATCTGGTCTAACAAAAATGTGACCTTCTGTTAAATCCATCCCTCTAACTCTTTCAAGTCCACTTAAAGCACCCGATGCTTCGTAACGATATAACCTTGATTGTTCTGAATATCTAATTGGCAAGTCTTTGTATGAACGTCTTGTTGAATTGAACAATATTATATGGTGTGGGCAAGTCATAGGTCTAGCAACTAATGTTTCGTTATCCATTTTAATTGGGGCAAACATAGTATCTTGGTAATGTGCTCAGTGTCCACTTATTTCATAAAGCTTCTTTTCACCAAAGTGTGGAGTTAGTACTTCTCTAAAACCAAATTTTTTATCAAGTTTTAAAACATAATCTCTAATTGCATTATGAATCTTCATTCCGTCTTCTAATCAAAATGGGAATCCTTGTCCACCTAATTGGTTAAAAGTGAATAAATTTAAATCTCTTCCTATTTTACGATGATCTCTTTCTTTTCTTTCCCTTAAGACATCTAAATATTTTGCTAATTCATCTTTTGTTTCCCAACAAGTACCATAGATTCTTGTTAACATTTTGTTTTTAGAATCACCTCTTCAATAAGCACCGGCAATTCCTAACAATTTGAAGTATTTAATTTCTCTAGAATTTTCAATATGGCCACCTGCACATAAGTCTGTAAATAGAATTTCTTTTGTTTTTGGATTTCAATATTGAAAGAATGTTATTTCTTTTCCTTCTTTTTTAAATTCTTCATAAAGTTCTTTTTTATATGGTTGATTTTCAAAAGAATATTGTTCAATGCCAACTTTTCGCATTTCGTAACCTTCTTTTGCTAATTCTTTCATTACTTTTTCAATATTTGGAAGATCTGATTCTAAAATTGGATTTTCAAATTCAAAGTCGTAATAGAACCCTTCTTCAATGGCTGGTCCAATTGCCAATTTAGCATTTGGATAAAGTTTTAATATAGCAGCCGCTAATAAATGGCTTGCTGAATGATTTAATTTTTTATTTGCTTCCATTATCTTAATCCTATTCCTTTTAATAACATATCTAAGTTAATTTGGGCAACTTCACTTGCCTTTTTTGCTCCCTCTTTGCTCAATGTTTCAATTTGATTCAAGGCATTTTTATACTTATCTTGGATCTTGATTAAAAATTCCTTTACACATTGAGCGACAGCATTTTTAAATTCACCGTAATTGCTGTTTTTAAATTTTTCAGCGCTTTCTTGTAATGAAATATCATTTAAAGCACTATATATAGTCAAAAGGTTTTTTATGCCCGGCTTTTCATCTGACAAATATATTTTTCCTTCTGAATCTGTAACAGCTTTTAATATTTTTTTGTATGCATCTTCTGGATCATCTAATAAATAAATTGCTGATTTAGGATTTGTATCAGATTTTGACATTTTCTTACTTGGGTCAGTTAAAGACATTATTTTGGCCCCAACTTCTGGTATATATGGTTCAGGTATTTTGTACGTTGTATTGAATTTATTATTCAATCTAATTGCTAAATTTCTAGTCAATTCAACATGTTGCTTTTGGTCAATACCAACGGGAATAATATCTGCATTATATAGTAATATATCGCCCACCATTAAGGTAGGATACATTAAAATTCCTGTTGGAATTGTTTCCATACCGTTTGCAGTTTTTATTTTAGCTGATTTATCTTTAAATTGCGTCATTCTTGATAATTCTCCGATTGTCGTATTTGTCAAAATTAATCAATTCATTAATCCGTGTTGAATGACATCTGATTGAAAAAATAACACACTCTTTTTAGGGTCAATTCCACATGCAATATAAAGTGCAAAAATATCCTTTTTATTTTTTGATAATTGCTTAGGATCAATTGGCAACGTTAGAGCATGTAAATCTGCTATAAAAATGTAATTGTTATATTCTTCTTGCAATTTAACCATATTTTTTATAGCACCAATATAATTTGCCAGCGTTAATTTACCAGTTGCAGTAATTCCACTTAATAACGTCTTCTTTTCAGAATTTTGCATAATTAGCCTTTCTTTTATTGTTTAATACAAATAAAATTTTATAACTTTCTAAGATTATAAAATAATTTTATTTTGCATTTATAAATTAAATAATATTAATTAACATTAATATATTTTTATTAAAATATAATTATATTGCTTGCATAAGTTATATAGGCAAAACTATAATAGGAGAAAAAATGCTGAACAAAAATAAATTTTTATACATATTTTTAATAATCATTACTCTTGGATTAATCTTAATATTTTGAAGAAAAAAATACAAGATTTTAAAAACAAAGAATTATTTAACAACAGACAATGAAATAACTTTTTCAATGCAAGATTTAATTTTATATTTGGGTGGAATTTCAAATATAGTTTCTTCATCTGCAACTCAAAAAATTGTAAGAATTTCATTTAAAAATAAAACAAATATTTCTATTGAAAAAATAAAAAAACTTAATGGCATATCTGGTATTGCATTTCAATCTTCGAATATATCATTAGTTGTTGGAAATTGTGCAAATTATTTATCTACATTACTTAATAAGGAACTAAACAATCATGAATAACAAAGATTCTAAATATCAAACATTAATGGCTAATTTATTGAATATTGATACATATGATACTTCACTATATACATCTATAAATAGTGAAAATTTTTTTGATATTTACAACAATTTTGGTCAAGAAATATTTGATCAAATTTTTAAAAATTACAAATTTAATTGTATTTTAACCGAAGTCAAAAATAAAGAAATGCGCCAATTGCTAGAAAAGGCACAAAATAAGCAAGAAATAATAACGATTTATAAAGATAATAATGTCAGAATCGAACAATCAATATTTAAGATGCTATCAACTGATTTTGAAAAAGCTAAAACCTTATTAATTACTAAATTAGAATCAGAATTTCAAAAATCAATAGTAAATTGAAAAAAAATAATAAATAAGGCTGAAAATATAAACATTGAAACAAATATCTGGCCTTTGCATGTAGGTTTTTTATTTATTTCAATAAAAACGGATAAAAAGACAATCTTTGCTCCCTTATTATTTAAAGAAGTAACATTAGAAGTTAAAAATTCTTTGGTTTATTTGAAATCGAATTCAGATGTAAGAATAAATAGTAAGTTAGTAACATTTTTATCTCAAGAAGGTTTTTTGTTAAATATTGATAATTTAGATTTTTCTAATTTGTCAATCGAATCTATATTTGAGCATTTAAAAAAAATTTGATCGCCTATATACAATATGCCTCAATCACTAAGAAGCAATATTCCGCTTGTATCTCAAAGTGATATTCAAAACGTATCTATCGAATTTCATCCCGGAATGATTTTTGGATTTTATAACGTTTCTTCGGGATATTTATGAAATCAAATGAAAAAAATTATTGAAAACGATGAATTTGAAAGTATTTTAGTACCCGAAATTAATAAAAATGTTTATAGAGAAAAAATCAACACAAGTATTTTTAATAATAAATTTAAGTTATTTAAGGTACAAAAAACAAACTTTTCACAAGATGTTGCTACAGTGTCTGCACTATGTCAAGATACAATAATTTGAGGACCTCCAGGTACCGGGAAATCTCAAACTATTTCTAATATTATTGTCAATATAATTGCAAGGGGATATACTGCGCTAGTTGTGTCTCAAAAGAAGGCCGCCCTTGATGTATTGAGAAATAGACTAGGACAATTGAGCTTATTTTGTTTATTTGCTTTAAATGATAAAAATTTAAGACAAGAGGTATTTTACGAACCATTAAAAAAATTTATAGAAAAAATTGAGAATTTTTCTATTTCTCAAGTTGAAAAAGGAATTCAAATTTTTTCTGATGAAGATAAGGGCTATGTCGATACATTGAATGAAATAGAAGCACTGCCAAACTTAAATAACTTACTTCATTTCTATGCCTCAACATTGAATGCCAATTTAACAATAAATGATTTTAATACCCTAAGATTATTGGCAAAAGATATAAAATATGATGTTGAAGGACATACTTTTAATGATAAGAAAGAACTTAAAAAATATCTATACGAAGTAAATTTGAAAAGAAGACCATCAATATTTTCAATTTATCCAAGGTCAATAAAAGAAGCTAGTGAATTAATTATTAATGCGCCTGAATTATTTAAAATAAATGTAGATGAAGCAGTAAAATACATTGATAAAGTAGAGTTTGCCGAATTGGAAACTTTTGTTGAAAAATATAAAAATTTACTGATTAAAAAAACAATAAATGTAAGTAACTCAGCCGTTTTATCTAATATGTTATTTGCTAAATTAATAAATACAATTTCGAATTTCAATGATGAACAAAAACGTGAATATACATCGTTTGCAATGTCTATAAGAACAGGTAGCTTAAAACCATATATGTTTTTCCATAGATATAAAAACATAATTAAAGTATTATTTCCAATAATTGTAACTACTCCTGAATTAGATTTGTCAATGTGAGGTAAAGAAGAATTTGATTATGCAATCCTAGACGAATCAAGCCAAATCTTTATTGAAAAAGGAATTCCAATTTTATACTTAGCTAAAAGAAAGATACTTGCTGGAGATTCAAAGCAAATGCAACCAACACGTTGATTTTCGGTTTCATATAATTTTGAAGATGAAGAAGAATTGGGTGATATTGAATCTCTTTTAGATTACGCTACTGCTAGAGGAACTTATTCAATATTGCTAGATAAGAACTATCGTTCAAAGAGGGCATCATTAATGACATTCAGTTCTAAACATTTTTATGATTCCAAATTAGATGTAATAGATGATTTTAAATTAGCATTTTCTAGTGAAAGACCAATTGATGTAATTCAAGTTGATGGCGAATGAAATAATAGTTATAATGAAGCTGAGGCTAAAGAAGTATTAAGAATAGCAAAAGAAAATCTAACTAAATATGAAAAAATAATTATTCTTGTTTTTAATGTTAAACAACAAGAATATTTGACAAATGCAATATTTAGCGTTGAACCAGAACTAGAAAAAGCCTTATTTTCCGATAAAATAACAATAAAAAATATTGAAAACATTCAAGGTGACGAAGCTGATTTAGTCATTATGTCTGTTGTTTATGACAAAAAAACAGCATTGTTTGGAACATACGTTGCAAGGAAGGGTGGGAAAAACGCCCTAAATGTTGCCATATCAAGAGCAAGAGAAAAAATTATTGTTGTAAAATCAATTTATGCAGATGATGTTGAAATTAATGAACGTTCAACATCTGATATGCGTTTATTTAAAGAATGACTAAAATTTTTAGATTTATCAATAATTGAACAAAAAAACTATTTATTTTTAGATAATGAAAATAAAAAATATGATGAAAAAACACAAACAATATTGTTACCAAAAGTAAGCAACCTTCAAATGGATGTATTGGCGAAATTAAACGAATTATTGAAGGAACTAAATGCTAACAATTTCGAAATTATTTCGAATTATTCAATAGGAACTAAATTATTAGACTTAGTCTTAATCAATAAAATTTCAAATAATATGATTATTGGTATCAATATTGACGATTTTAAATATTTGCCTGATTATAGAAAATATTTAAAATTTAAAGACGATTTTAATTTTTTACTATCAAAATCATATCCAATAATTAATATTTCTAGAATAAGTTGAATAGTAGAAAATAAACAAATTTTAAATAAGATTAAAATTTTAATTAAACACGAAGAAGAGTTAAATTCTCAAATTTTATTGTCTGAAGAAAATAACAATTTAAATGTAGAAGAAATTAAAGAAAACATTATAGAAAATAATGCCTTAAAAAACATAAAATCAAGCCAAATTAAACAAGGTCCTGAATTAAAAGAAATAGATTTTGAAAATGTTGATGACGAAACTTTTGAAGTTGATCAAGATTCTAATAATAAAATTTATGAATTAAATGAATCTGAGGTTGCTCAATTTGAAGAATTCCATAATAAGAATATAAATCATGAATTTAATGACCAAACCGAATCAAAGGCTGAATACTCAAAGGACTATGAAAAAGATTTTTTGATTAATTATAATGATGTTGAATTGGATAATACGCATATCGATCAGGAATTTCACATTGAAAATGAATTTGAATCAACAATAAATAATAGTAATGTCATAAAGATTGAAGATGTCACTTCATCATCGGCTAACGATGAATTAAAAGTAACTAATTCTAATGAACAAAAAATTTCTGCAAAAACAGCAAGTGAAGATATTGAATCGGGATTTTCAGTTGAAGATATAAACAAAGAAAATATTAGCAATTTATTTTCTAATACATTTGATGATCTTGATATAAATATAGCACCTGAAAGTGAACAACAAGTTGAAGCTAAACTTAATTATGATTCAGGCATTATTCAAGATAAGTTGCATGAGCAAAACGATGAATATTTTAATTTAGAAGAAAATAATATTGAAGAGCAACCAAAAAATAATAATGTTCAAAGCGCAGATGAGACATTAAAATCTCAATTAGTTTATATTGACAATAATAAAGAAGTAAACGATAAACAAAAAAGCTATGATTTTGCAGGTTTATTTGCTGACGAAAATGAAGAAACCTCTACAGATGTAAAAAACATGATAGACATGAATACTGCAGAATATAATCAATATATGAAAAATAAAGATATTGAACAAGATGAACAAAATAAAGAGATAACTAAAAATACTGCTGATGAAGAAATATTGAATTTTGATTTAATCGAAGATGAAAAAACTACGGAATTTAACGTGGAGGACAACAATGTCAAAAAATAGCGACCCTCTTGATCAATGAGAGCAAGAAATAAAAAGAAGAGTTTCAATAGAAGATCAATTATTGCATAAAGATATTGGAACTAAAATTGCAAAGCAAAAAAAGATTAAAAAAATTTTGTTCATAACTTTTTCTGTTGTTGGTATTGCTACAATCGTAGGAGCAACAATTGGAGTTACAAGCAGAAAAAAAATGGAATTAGTAACTTATGATGATTACATTGATGTTTCTCTATATTCACAAATAAATTCTAAATTTAAAGAATTGTATCTTGACGGAAAACAACCTGACAAAAAAAATTACCCAATTACTTGAAATGTAAACACTAAACCAATTTTAGGTGAGCCCTTTGAAATTCTAAAAAAAATATTAAGCGAGGGTGCTAATTTAGACAATAAAATTTTTAAAGAATTCAATTTTAAATATGATTCTAGTCAATGAAAACCTAGATTAACTAATTTCTTTTTAGATGCAATAAATAAAAAGCATTTGCATTTTGATATTTATTTAGAAAACAAGAAAAATTTAAATCAATCATATGTGGTAAGAAATTTTACTATTGAAACAACGAATGTTTTAAATAATGAAATGTTTTCTGAAGCACAAATTGCCAAGTGAAATGAGCAAGATCCCAATATTTCTAAGGCGGCTTTAACTAAAAATTGAAATTTCTATTATGTTACTAAAACAAATGCAAACCTAAATTTTGAAAATGATAAAACATTTGAAGAACTTAGAAACAAAATAAGTAATTTTAATAATAAAATTTTGAATAATAGTGATTATAATTACGTTGATAAATCGCTTTGATTAACATCTCATCCTAAAAAAACCGATGAATCCGAATTAAATTATTTTAAGTTGTTTCAGTCGGATGTAAGAGACTGATTTGTTCCATTTAATTTCTTAAGTCAAGGAATTCAATTGAAGAAGATTGAAAAAAATGAAAATAATATTTTTACTAAGATGCTCAATTTAGAATTCAAGTCAACTTTTTATACTATTGTAAAGAATAAATTAATTTTAAAAAATAAATCTAGTTATGAATATGCTATTTATGTTCCATTTTTTACAAATAAGGATAAGACCATTAAAGACGGCAAGGAAATTTATCAACCTAGAGTTTTTGAATATAGTTGTCCTATAACATTTGTTAATAGTAATTACAAAAAATTTAAATAAAAAAACCGTTTAAGTCGGTTTTTTTATTTAACTAAATTAAATGAGAATTTTTTTCTTGCTTTCAAGGTTGATTTTTATTGATTCTATCTATAAATAATTTTCCTTGTAAGTGGTCATATTCATGTTGCAATACAATTGCCGGATAGCCGGAAACATCATAAATTTTTTCTTTTTTTTCAAAATACGAATATGCTTTTATTACAACTCTTGAATATCTATATACAAAACCTTCTTGACCTGCATCGCTTTCCTTAACACTTAAACATCCCTCCCCTTCTTGCAATGATATTTTATGGTTTGAATGTGAAAGCATTATTGGATTTATTAAAGCGTCTCTAAAAATATAATTATTTTGATCGTCTTTTACCATTACATAAAAAATATTTTTCAATATTCCATATTGAACTGCTGCAACTCCAACGGCAGGTCTAAATTTTGTTTGAGGAGTTTGACTATCATCAACATGGAAAATCATTTTTTGTATTAATAAATCATCTTCTGCGGTCAATGGGAGTTCAACGTTCTTTGATTTATTTCTCAATACTTTTTCTGGTAATTGAACTAATTTAACATCTTCAAATTTAAACATAGTTAAATTATTACATATTTAATTGAATTTAAATGAACTTTTTAAAAATATTTTATTATAATTAGAGATTATTTTAAATATAGGAGAAATTAATGCCAGAGCCTTTATCCGGTAACTATAATACTTTAAATTCAGCATCAGGAGCTTCGACTTCAACCGGTGCACCATGACAATACATTTTATATTCAATCATTCTTTTTTTACTAATATTATTTAGCGCTATATTTTCAGCTTCAGAAACAGCATATACAACCGTTTCAAGGGCTAAGATTGAAAATATGATTGAAAAAAAGCAAAAGTTCGCTAAATTAATTCAAAAACAACATTTATTTTTTAATAGAATTTTAGGGACTATTTTAATAGCTAATAATTTGGTAAATATTGCATCTGCAACATTGTTTTCATATGTTTTATCATTAACAACAATGTCTTCTGGCAAGGCTACTATTATTTCAACTGCTGTTATGACTCCACTTATTGTTTTGTTTGCAGAAATTTTACCAAAATTAATTGCAAAAGCATATCCTGAAAGGGTTATTAGATCAACTTGTTGATTTATTGAAGCTTTATATTGAATATTTTTTCCAATTACTTATCCAATATCTAAAATTGGCAAAAAGATTTATATAACAAATACAGAAGAAGATATGAAAAACTTGATTAATATTGCACAAGATGAAGGTGTTTTAGAAATCAACGAATCAACTATGGCAAAGAATGTTTTGGATCTTGATTCAAGCAAGGTTTCTCAAAATTACGTTAAGTTAAAAGATATTGATTATTTGAATTATAAAGCAACTTTGCAAGATGCATTAAACAAGTTTAAAGAAACTAATTATTCAAGATTGCCAATTGAAAAAGATGGAGATATGATAGGCATATTGCTTTTAAAAGATATATTCTTTTTAAAGCGTGGCAATATTATGAATTATGTTAAAACGGTGCCAAAAATTTCTGCAAATAGTTTACTTTCTATTGCATTAGAAAAATTAAGAAAAGATAGAGCACAAATGGGTTTCGTTATTAAAAATAATAATGATGACAGTGTCATAGGAATAATTACAATCGAAGATATTATTGAAGAAGTTGTAGGCGAAATATATGACGAATATGATGATGATGAACAAATTTATGAAATAAGTTTGGAACGCTGTGAGGCCCGCGGAAACGTTAAAATTTCAACAATTTGAAAGCGCTTTGAGTGAGATGAAATTTTAAATTTAGAAGCTCCTAATGATGAGGATTTAAATTTATCAAATTGACTAAAAAACCGTGTTGGCCACGAGCTACATGTTAATACAAAATATACTTACGAAGATAAAATTGATTTTCAAGTGATATCATTAAAAAATAAAGATCAAAAATTTGATATTATCGATATAGATTGAAGTAAATAAAACCTGTTTTTCTTAACGGGTTTTTTATATCTTTATTATTTATTTTATAAATAAACATTCATTTTATACAAAAAAACTATTTTGTGTATATAATGTAGAAGTTAAAAATATTTTTAACAAGGTTTATGACTCCTTGACCGTTAGGTCCAAATGTCCAAAAGGAGAAAAATTATAATGTCAAATTACGAAATTATGATTCTAACTAATCCAAAAACAACAGATGAAGAATTACAAAATCTAGTATTTTCAGTATTAGATAAAAATTCATCAAAATTTGAAAGATTAGAAAGAAACGAAACTGCATATCCAATTAAAAAGTTAACTCATGTAAATTACTTTTTAATTACAACAAAGGCAGAACCTCATCTAATGTCTGAATTAACAAGAAAGCTTAATATTGATAAATCAGTTTTAAGATCTTTAATTATTAATTTAGATTCTGAAAAAGGTTTAAAGCCAAGAAAAGTTTCTAAGAAATTCAATAAGAGAATTCCTCTAAACCGTGAAAATAAAAAACCTTATGTAAAAAACAACGAAACTTCTAAAGATGCTAGCGAAGAAAAGAAGGTTGCAAGAAAACCAAGAACTTCAAAAGTTTTAGACGAAAAGAAATAGTTAAAAAATAATAAGATCGGTAGGAAAAATGAATAAAGTATTTTTAATTGGTCGTTTGTCGGCCAAACCTTATAAAAATGTTACACCTTCTAATGTTGAATATTCAAGATTTACTATGGCTATTAGACGCGAATACGCCGCCCCTAATTCTGAACCTGTAGTAGACTTTGTCCCATGTGTTGCTTGAAGATCTAACGCAACTTTTATTAATAAATATTTAGATAAAGGTTCGCTTCTTCTTGTTGAGGGTACATTTCAATCTTCACGTTTCAATAACGGCGATGGTCAAATTTCAACATCATATGTTGTTAGTGTAGAAAAAATTCAATCACTAGAAACACGTGAAATGTCAGAAAATAGACGAAAAAATAACAATGGTAAAGAATTTAATATTGCCACAGAAGAATCAAACACTATCGATAATAATTTAGAAAATAATCATCAATCAAATGAATCAAATGATAATCCTTTTGATCAACTTGATTGAGATTTATAGGAGTTAAAATGGCAAGAATTGTTCGTAAGAAACCTTTTATTCGCAAACGTCCATGTCAATTTTGTTTAAGTAAAGAACCAGTTGTTTACATTGACTACAAAAATGAAGAAGTTTTATCAAAATTAGTAAATTTACAAGGAAAAATTTTATCATCACGTATTACCGGAACATGTGCAAAACACCAAAGAGCAGTTGCATTAGCAATTAAAAGAGCAAGATTTGTTGGTATTTTGCCATACATCGGTTTAGTAAAAAGAGAATCTAAAAAAGAAACTGTTAAAACTGAAAAAGTAGAAAAAGCAGAATAAAATAATAAAAAAATCAAGTGTATGTATTTGTTATGTAACTTGATTTTTTTATTATAATTTTTAGTAATCATGAATATAAAAACCAAGCAAAAACTAGCAATATTTATTGGCGTTGTCAATTTTTTAACAATCATAATTTTTTTATGTTGATGTTTAACTTATTTTTTAGAAAAGAAAATAGATTTTAAATTATTTGGTTATATTTGTTTAGGCTTTACATTGGTTGCTTTTTGTGTAAATATAGTTGGATTAATTTACACTACAAAATATGATATTAAAAAACCTTGAAGAGTTTTTTTTATAAGCCTTTTAATAATACCTTTAGGATTTTTTGGGGCAATAAGCTTAGCGTTGAATTATCCAGAAATTAAAAAAAATAGGAGTAAGAGTTAAATTAATATGAATAAAAAAATAAGTTTTAGCCAGATGATCTTAAATTTGGTGGCATTTGTTTGCTTCATCGTACTTATTTGATCTATTGTGGCCAATATTTCCTTTAAATTTACATATGAAAAAAATTTTCAAAATCCAATTTCGGTTTATGAAACTAATAAATTTAATATGATCAATAGAGTCTTAACGGCTTTTTTAGTATTATACATTTTATTTACTATTGCTTCTATTTCACTGAATATTTATGTTTCAATAATCTTATTCAAAAACAAGCATGCAAAACTTTTTTTACTTTCATTAGCAAGTGTTTTAGTTTCAAGAATAATTGGTATTTTTTGTGGAATCATTTTGCTTTTAAATAAAAGACTTTCAACAAGCAAATGCTAAAAGTCTTTTATTTTTTAATAAAATAAAAAAATTAAATATTTTATAATTTAACCATATGGATTCTAACTTAGCAAATTTAATAAGGCCCAAAATTTTAGAAGATTTTATTTGCCCACAAGAACAAAAAAAATTATTTCAAAAAATTATAGAAAATAATGATTATCGTTCATTTATTTTTTACGGAAAGCCAGGTACTGGAAAAACAACTATTTCCTATATTCTTGCCAATGCTTTAAAAGTAAATTACGAATATTTTAATGCGGCCATTGAAAATAAAGAAGATCTTGTACATAAATTAAAGTTGAACAAGATTTTGATCATTGATGAGATTCATAGATTAAACAAAGATAAACAAGATATATTGCTACCATATCTAGAAAACGATTTAATCACAATTTATGCAACTACAACGGAAAATCCATATTTTAAGCTTAATCCTGCATTACGTTCAAGATGTTCAATAATTGAAATTACTAAGCCAACAATTCAAGATTTATCAAGTTGTTTAAAAAATGCCTGCCTTAAAACTTTTGGTGTGGATCTTAATAAGGATATTGCTGATTATTTGGCCTTGCAGGCTAATGGCGACTTTAGATCGGCATTAAACAATCTTGATTTGATTATGTTAATTTTTAAAGATCAAGAATTTTCATTGGAGGCAATTAAAAAGATTATTCCCTCAATTCAATTTAGTTCAGATGAATCTGGCGATAATCATTATGATTATTTAAGTGCTTTTCATAAATCACTACGCGGTTCGGACATTGATGCAGCATTATATTGAGGATTTATCATTATTAAATCCGGAGATTTTGATGGTTTGTTTAGAAGAATGTTGTGTGCAAGTTACGAAGATGTTGGAATGGCAAATCCTAAATTGGGATCAGAGGTTTTGTCGGCAATTGAATGTTTTGAAAGAATAGGAATGCCTGAGGGGTATTTACCAATAACACAAGCAATATTAAATATTTGTTTAAGTCCAAAATCAAACACTGTTTATAGTTCATCAACATTAATAAAATCTATAATTGACAGTGGAAATATTTTTGAAGTGCCAATTCATTTAAAAGATGCTCATTATAAATCGGCAGCAAAACTAAAAAGAGGATTAAATTATAAATATCCTCATGATTATAAAAATAATTATATTGATCAACAATATCTACCAAATGAATTAAAAGATAAAATTCATATTGAATTAAATGGGCAAGGTTATGAACAAAAAATTAAACAATATTGAGATTTAATAAAAAACAAAGGAGAAAAATAAAATGGAATTTGATTTAAATAAAATAAATACATTGGAAGATTTAAAGTTAGCTAAAAATGCTTTTAACAATTCAGATGAGTTAAAAGAATTAATGTCTCAAATTAAAACAGCAGATCCATTGCAAAAAGCAGAACTTGGTAAAAAAATTCAACTGTTAAAAAAGCAAGCAGAAGATTTTTTTGATTTAGCAAAGCAAAAACTTGAAGATATAAAAATTCAAGATTTAATTAATCAAGAGTATGTAGATTTTGCAAATCCTATAAATACAACCGGAGGAATTCATCCAATTAATTTGGTTGCAAATAGATTTAGAGAATGATTATTGGCAAATGGATATTTTGAATATACAGGGCCAGAAATAGAAAATGAAGAATATAACTTTGAACGTTTGAATATTCCTCAATCTCACCCTGCAAGAGAGATGCAAGATTCACTATATATATCAGAAAAAGAATTGCTTAGAACTCACAATACGGGATTTAGTGCAAGGGCATTAGAAGAACATAAAAATCAAGAGTTCTCACAATTTGCTATTGGAAAAGTTTATAGAAATGATGAAGAAGATAGAACACATACTCACCAATTTACTCAATTAGATTTTGTTAGTGTTGGCAACCATTCATTTGGAACATTGATTTATACATTGACTGAATTATTTTCTTATGTTTTAGAACAAAAGGTAAAAATTAGACTACGTCCTTCTTATTTCCCATTTACTGAACCTAGTGTTGAGGTTGATGTTTTGTTTAATGAAAAATGAGTTGAAGTATTGGGTGCTGGTATGTTGCATGAAAATGTTTTAAAAATGGCCGGATATACCAATGATATGAATGGCATTGCTGCAGGAATTGGTATTGAAAGAATAACAATGATAAAGTATGGCATTGACGATATAAGAGAATTGTATGGAAATGACCAAAGATTTTTAAAACAATTTAAATAAAAGGAGCAAAAATGTTATTTTCTTATAAAAAAATAGTTAAATTGGCAAATATAACTGATAAAAGTGTAGAAGAGGTTGTTGGAGCAATTAATTCAATAGGCTTTGAAGTTGAAGAATATCATAAGTTTTTAGATGCTGAAGGTATGAAATTCTGTCATGTATTAAAAGCATACAAAAATCCTAATTCTGATCGTTTAACGGTTTGTGAAGTTGAATTTGGCAATGGAAATCATTCAATAATTCAAACTAATGCAACAAACATGCATGAGGGCGATTATGTAATGGCTTATGTTCCTGGATCGAGATCTGGAAAAAATATATTTGCTGCCAAGGAATTAAAAGGAATCATCTCTGAAGGAATGTTTGTAAGTTTGAATGAAATTGGTATTCCAGAAGAATTTCATCCCTTAGATTCAAAAGATGGTATTTTTCAAGTTGGAAAAATAGATTTAAATTTAGACCCTGTTGAATATTTTGATCTTGATGATTATTTGATTGATGTAACAATTTTATCCAATAGAGCTGATGCTTTGTGCTATTTAATTTTAGCTAAAGAAATTTCAGCATATTTTGAATCTTATATTGAACCTCTTTCAAAACCAAAACCTACCTTGGAAAGCAATATTGTTGTAAGCAAAAATTTAGTTGCTACAAACTGTTTTAGTTTAGTTGAAGCTATTACTAAACAACCTGCTATTTCTCTTCAAGATGAATTTCTTTTATGAAAACACGGAATAAAAACATTTAGAAATGCAGTTGATATAACCAATTTGGTCTTATTATACGCAGGAGTTCCTTGTCACGTATATAATAAAAATTCTTTAAGTTCAAATGAGTTTTCTACATCATTGAGTAATGAAACATTAAATGTTTTAGGCAATAAACAGGTTACATTAAACAACAATTTGGTTGTAAAAAACGGAGATCAAACCGTTTCAATAGCGAGTGTTATAGGCTTAGAAAATTATGAATATAGACCTGATGATGGACATTGCGTCTTTGAATTAGCATCATTTTCATTAAAAGAAGTTAGAAAAAGTATAAAAACAGTCAAATTAGAAACGTTAGCATCTTTACGTGGTTCAAAAGAAATATCAAGCGGTCAAGTAGTTATGGCATATGAATTTTTAACTAAATATTTAGATCAATATTCAGTTCAAATAAACGCGCCTAAGCTAAGAAAAACAAATATATTAATTGATAGCCATTATATTACTAAATATGCAGGCTTCAATATTACAAAAACAAAAAAATATATTCAAGTTTTGAATCAATTGACTCTTTTGGGCTTTAAAATAAAGGAAGATTTATCATCAGTAATTTTTCCAACTTATAGATACGATTTAAAAAATATGCAAGATTTTGTTGAAGAAGTGTTTAGATTTTATGGATATGATAATTTCCCAGCTAAATCTCCAAAAATTACTAGATTATTAATTAAAGAAAACAAAGAATTTAAGTTTATTGAAAACTTCTGTGCAAAAGGATATTTCAATGTTAGAACATACACGTTAATTAATCCTGAAAAAAACATTTTTAATCCATTTGATTTTAAAACTAATTTCAATGCAATTGCAGCAAAGAATTATGATCATTCTCAAATTAGAAATTCAATGATTTCTTCATTATTAGAAGTCTTGGATACAAATATTAAACAAGGTTTGCCAGACGCTTCATATTTTGAAATAGCTATGATTAATAATAAAATGAATGTATTAGGAATTGCTTCATTAAATAAAACTATTGATGATATAAAAGCTGATATTTGTTCAATTACTAATGAAATATTAGAATTTAAACCAAGTAAATTAAAAATTTTTAATCCAAATGCAGCACAAGAAATTTATTTAAATAACGAGTTAATTGGATATGTTGCAAAATTAGACCCTCACTATTGTCAATATAATGCAGTGTTTAGTGAAGTCTTTTTGGACAAAATGAATAGTAATGTAATTAAATATAAAAATTATAAACATACACCTCTTAAGTCTAGAGATGTTACTATATCTGTTTTAAATAAACAATCTATTGAAGAAACAATTAAAAAAATACACAGTATTTTTGGCGTGTACAAAATTTCAATTAAGGATGTTTATAATAAAGATAATGGCACAAAAAATATTACATTAGAAGTAATTTTAGAAGATTGAGCAACCAAAAAATTTGATTCAATTTTTAATAAATAAAATTAAAAAATGGTAAAAAAAGCCATTTTTTCTTTATTTTTTCTCTATTTTAAATTTTTAGGATATTTTATGAATATACTTAATTGCAGGAGTGAGAATGAAAGAAACAACAAATGATAATCTTCAAAAAGAAAAGAGCATTATTGCTGAAACATTAAAGGAAATAGAAAAGAAATTCGGCAAAGAAGCCGTTATGATTTTAGGAGAAAAGCCAAGCATAATTCCTGAAACTTTTAGCAGTGGTTCGTTGACTATCGATAGAGCGTTAGGAATAAATGGATGACCAAAAGGCAGAATTATTGAAATATATGGTCCAGAAAGTAGTGGAAAAACAACATTAGCGTTGCACGCAATTGCTGAAATACAAAAAACTGGCGGAATTGCAGCATTTATTGATGCTGAGCATTCTATAGATCCAATATATGCCAAAAATTTAGGGGTTGATATTGACAATTTAATTTTATCTCAACCAGATTCAGGAGAACAAGCATTAGAAATTGTTGATACATTGTCTAAATCCGGAGCAATAGGACTAATCGTTGTTGATTCGGTTGCTGCATTGGTTCCTGAGGCTGAGTTGAATGGAGAAATGAAAGACCAAGTAGTAGGTGCACAGGCAAGGCTTATGTCTAAAGCTTTAAGAAAAATAACTGGATCATTATCAAAAAATAAGACAACAGTAATATTTATTAATCAAATAAGAGAAAAAATTGGAGTTATTTTTGGCAATCCTGAAACAACAACTGGCGGAAGAGCATTGAAATTTTATTCAAGCATCCGTCTTGAAGTTAGAAAAATTCAAAATGTAACCAATAATGGAGACATCACAGGTAATCAAATTAAGTGTAAAGTTGTAAAGAACAAATTAGCTGCTCCATATAAAACTGCTCAAATTGATATTATTTTTTCAAAAGGTATTTCAAAAGAGCTTGAAATAATTAATTTAGCAGAAGAATTAGGAATTTTAGTAAAAAAAGGCTCGTGATATAGTTTTAATGGCGAAAATATAGCACAAGGGGTATTAAATTTACAATTTATTTTGCAAACTAATAAAGAATTATTCGATAAATTAAAACAAGAAGTTTTAGACAAATTAAATTCGATAAATTAAAATAGAAATTTAAACATATAAATGTTTATAATTTAAACTATGAAAAATGAAAATATAAAAATTCTATTTTTGGGAGACATATTTGGTTTACCGGGAATTAGATTTGTTGAGCAACAATTGCCAAACATAATAAAACAAAATAATATTGATTTTGTAATCGCTCAAGCAGAAAACGTTTCGGGAAGAAAGGGTTTTGAGCCTAAAGATTACGAAAGATTGAAAAGAGCGGGAATAAATGCATTTACACTAGGAAATCACGTTTGAGCCAAAGAAAAGATTCATCAAATAATAAACAATGGAGATTTAATAAGACCAGCAAATATAAATAGTTCATATCCTGGTAAAGGCATAAGAATATTTGATATTAAGGGTCATAAAGTCGCAATAATGTCTTTTATGGGTATAACATTTAATCCTCTATTAGAACCATGAAAAGAACAAAGTGCAAATAATTTCTTTGATGAATTTGATGAGTTATATGAAAATAATGGCGCAGATTATTACATAATAGATTTCCATGCTGAGACAACATCTGAAAAAGCGGTATTTTCACTATACGTTGATGGTAAAGTTGATGCAATTTTCGGAACGCATACGCACGTTCAAACAAACGATGCAAGAATTTTGCCTAAGGGTACATATTTTGTAACTGATGCCGGAATGTGTGGCCCAAGAGATTGCGCTATTGGTTCTGACTTCCAAGAAGTATATGAAAAAATGCGTTACGCAGCATTTTCTGCATTTAAGGTATCAAAAAATAAATGTCAATTAAATGGAGTTATTTTTACATTAACCAAGGATCCAGCAGAAAAGCATATTGAGTTAGTAAATATAAAGGAGCAATAATATGAATAACGATTCATCATCAAAAATCAGTGAGAAGGATAAACGAATTTTATTTCTACTCAATAAATATAAGGCAAGGATGCATATTATTAATTGCATATATCAGAGCGAGCTATTTAATAAGGATCTTTCAATACAAGAAATTTTGGAAAATTGTGGAGAAAATCAATTGAATGCGAATGAAATAGAATCTTTAAAAATAATTGAAGCTAAATACGATGTTTTTACTAGAATAGCAAAAAAATTTATTAGTGAAGACTGAGAATGAAATAGAATTTTACCACTATCGAGAGCCATAATAATATACGGTGAATATGAATTATTATTTAATGACCCAAGGGTGGTAATTAATGAGATGGTTAATATTTCAAAAATATACAGTCCTAATAATGATTATAAATTTATCAATAAGGTTCTAGATCTTGTTTCTAAAAATGTAATTAAAAAAAATACTAAAGATGAAAAAGAAAATTAGCGAGATAATAACCAATATTTTTAAATACGATTTGAAGATTTCAGAAGCGTTAATTAGAGAAGGAAGAGTAATTGTAAATAATGAAAAGATAATTATTCCATCATTAAAATTTGAAGAAACCGATGTAGATATAAGATTCATTAATATGAAATTGCCCTATGTTTCAAGAGGCGCTTATAAATTATTAGAAGCAATAGAAAAATTTAATATAAACTTAACGGATAAAATATGTCTTGACATAGGATCGTCTACTGGGGGATTTGTTGATGTAATGTTAAGGCATGGTGCTAAAAAAGTATATGCCGTAGATTGTGGCACAAATCAACTAGCATATGAACTTAGGATAAATGATAAGGTTGTTGTTTATGAAAAAACTAATCTTAAAAATTTAAAAAAGGAAATGTTCCTTGAAAATATTGAATTTGTATCTTGTGATGTTTCATTTATAAGCTTGAAATATGTATTTGAGGTTTGCAATAAAATATTAAATAATAATAATATAAATGTAGTTTTTTTAATTAAACCCCAATTTGAAGCTTCAAGCAAATATGTTGAACCCGGAGGATATGTTAATCAAGAAAATCATAAATATATTATTGATAAAGTAATTTCATATGCAAAAAATAATGGCTTTGTTTTGATTGATTTAATAAAAAGCCCTATTTTAGGAGAAAAATCAAAAAATATTGAATATTTAGGCTTTTTTAGAAAGGATAGTTAATGAATTATAAGGATTTATTTCCTATGTTTAAAAATAACCCTGGTATTGTATATTTCGATAATTCAGCACTTACTTTTAAACCGCAAACCGTAATAAATTCAGGTATTGATTTTTATGAAAAATATTCAATTTCTACAAGGACTGCTGATAGTGTTTTAGGAATACAAACAGAAGAAGTAATGCATGAAACTAGAAAAAAAATTGCAGATTTGATTGACGCTTCTGCCGATCAAACTATATTTACTTCAGGAACGACTGATGGATTAAACTTAATATCTTTAATGTTAAAAGATATAATAAAGGATGGCGAAATAATATTTAGCTATTTTAATCACAGCTCAGCAATCGTTCCATTCATTGAAAATCTAAAAAACAATAATATAACATTTAAATATGCGAAAGATAACGAGGAAGTTTTAGATTTAATAAATGAAAAAACGAAGTTAATTGTATTGCCTCAATTAACTAATAATTTTAATGTATTTTTTGATTTAAAAAAAATTCATCAAAAATCTAAAAAATATGGTGCATATTTGATAAACGATGCCGCACAAGCTATTGCTCACGAAAAAGTTTCATTGAACGACTGCGATGTAATTGTTTTTTCAAGTAATAAAATTTATGGTCCTACCGGTATGGGAGCTTTAGTAATTGAAAAAGATTTACTAAAAAAAATAGTTCCTCAAAAATGAGGTGGTGGTCAAGTTCAATCTATTGGTTCTGGTTGCGAATGAAGTGCCAAGAATGATATAAAAAAATTTGAACCTGGAACTCCTAATTTTGCCGGGATTTTTCAATTTAACGCTGCAATTGATTTTATAAATAGTCTAGGTTATGAATTAATAAATAAATTAGAAACAGCTGTTGCAAATTATTGCTATGATGAATTAAAAACAATAGATGGAATAGAAATAGATTCAAAAAGAGGGGATAAAATTATTTTATTTAATATAAAAAATATTCCTTCTCAGGATATTGCATCATATTTAGGACATAATAATATTTATGTAAGATCTGGGGTTTTTTGTGCACATAAATTTCATGAATTAAAACAATATAGCAATAGTTATGTAAGAATTTCAATTTCATTTTATAATGATTATAATGATGTTGATAAATTCATTAAACTTTTAAAAGAAATGGGGGCTAATTTTCTTGAATTTTTATAGTAATATTGAAAAACAAAGAATAATTTTTGAAGCATACGAAAACCCAAAATTTAAAATAAAAAATGATGAAGATTTTAAGAAAACTGATAAGACATTATTTGAACATTCAAATGTATGCGTTGACGATATTAAGTTAAATTTTTTATTCGATGAAAAAAATACTTTGAAAAAAGTGGAATATTGTGCTAAAGGGTGTTCAATTTTTTTGTCATCCGTTGAATTAATGATTCAGCAAATTTTGAATAAAAATAAATTAGAAATAAATAAAATTTTAAATGAATATAAAACAATGATAGATACATCTACAATTTCACAACGAGGTGGAGAAATATTGGGAAAATTGGTTGTATATCAAAATGTAAGAGTTCATTTAAATAGGTTAGAATGTGCATCAATAATTTTTAGGTTAATAAAAAAAGCAGTTTCTAATGAATAAGATAATTTTTCACATAGATATGGACTCCTTCTTTGTTAGTTGTGAACGTTCTATTAATACAAACCTGATAGACAAGCCTGTTGTAATTGCTAAACGATACAAACGTTCAATAATTAGTGCAATGTCTTACGAGGTAAAAAACCTTGGATATAGAGTAGGAAGTTCTTTATACACCGTTTTAGAAAAGGTTCCTAATTTACTTATAGTAGAACCACACTATGATTTATATTCTTTAATTTCTAAAAAAATTTTTGATTATTTAAAAAATAAGTATTGTACACAACTAGAAATATATTCAATAGACGAATGCTATATTGATTTTAGCAACACTGTTAGTAATTGGGAGGAAGCTAAAAATATTGCCCAACAAATTAAAGATGATATTTTAAAAACATTTATGATTCCTTGCTCAATAGGAATTTCCTTTACAAAGTTTTTGGCAAAAATGAGTACAAATAAAGCAAAACCATCAGGAATTTCAATAACAAAAAAGGAAGATATTGAAAGAAATTTTTACTCATTGCCGGTGGAAAAAATATTTGGGGTAGGAAAAAATATTTCGCAACAATTAATAAAAAATAGAATTCTTAATTATAAAGATTTGCTAGAATGCAATAATGAAATTTTTTTAAGAAAATTGTTTAAAAAAAATTTTTTCTTATTTATTGAACAACTAAAGGGAATCAATGCAACCGACCACATCTTAAATCTTGAAACAAAAGGAATAAGTAACACGATAACTTTTATGGAAAAAGATTCTGATGATATCGTTTTTTTGAAAAATGAATTGTTTAAATTGGCAAGAAATGTTGCTAATAGGGCCGTAAACAACAATTATGAATGCAGATGTGTTGAAGTTTTAATAAGAAAACAAGACAAAAAATGAATGTCAGAGCAAAGAAAAATTGATTTTTTTACAAATGATATTGACATTATTTTTAAAATTGCATTGAAACTTTTCTTAAATTTATGAAATGAAAAAGAATGTTTAAGAGGTCTTGGAATTAGAATCTGTGATTTGAGGTCGATATTTCAAGAAAACAAACAAGTTAATTTGTTTGAAGACAATAATCAAAAATCAATAGTAAATTCAATATTAAATGAGGTTAATACAAAATTTGAGGGTAAAATTTTAATAACAGGGAAACAATATTTGTTAGAGAATTCAATTGATATTGATAATATAAAGTTTTTAAGAAAAAATAATCAACCCTATGATAAAAAAATAAATTTAGAGGAGAAAAAATAATGAAGATAGGAATATTTGGCGGAAGTTTTGATCCTGTTCATAAAGGACATATTTTAATTGCTAATGATGCAATTGAATTATTAAAACTGGATGAAGTTATTTTTGTTCCTGCTAACAAAAATCCTTTTAAAGATAAGCAAGATTATGCATCTAATGAACATAGAATAAATATGATTAATATTGTTATAAACAAGAGTAATATGGAAGTTTCACAATTTGAAACAAAAAGAGGTGGCACTTCATACACAATTGATACTGTAAAATATTTTGCTCAAAAGTATCCAAACGATGAGCTATATTTTTTAATTGGATCGGATAATGTTGGTAGTTTAAACAAATGAAAAGATATAGAAGAAATTTCAAGAATTGTAAAAATTGTTGTATTTAATAGAAATAATATTTATTCAAAAATCAATATTAAAAAATACAATTGTTTAGTTTTAAAAAATTCTTTTCATCCTTTTTCTTCAACTTCTTATAAAAATGGAAATATAAACGTAGTAGATGAAAGGGTTCAAGAATACATAGGAAAGAATTGTTTATATTTAGAAAATATTGCCAAAAATACTCTTTCTATTCCTAGATTTAAACATTTAAGATATACTGCTGAATTTTCTGCACAATTGGCTAAAAGAAATAATTTTGATGTCGAATTAGCGTATAGAACTGGTTTTATGCATGATATTACTAAAGAGTGAGACATAAATAAAGCATATAAATTTTTAAGTTCATATGGGTATAATGAACAAAACTTGCCAGAATATATGCTGCACCAAACTACGGCCTATTATTGGTTGAGGGATGTATATAAATATCAAAATGATGAAGCGTTACAAGCAATCAAAATTCATACATCATTAGATTTTAAAATATCTTTATTGGCAAAAATTTTATATATTGCTGACAAAATTTGTGAAGGAAGAAAATGACCGGGAATTCAAAAAATCAGAGAGCTTGCCTTAAATAATTTAGAAGAAGGTTTTAAGGAAGTAATTAGAATATGCGGCCTTAAATTTAATGAACAAAAAGGCATTATTTTTGATGAAAAGCAAAAAGAAATATATAAGTTATTATTAAAATAAAATGTGTTATAATGTATATGCTTTAACTAGCATAAAGTAGATTCATTTCTCACCTTAGTGATTTTTTGCTGGGTTGCTACAATAAAGAATTTGTAGTTTTTGGGAAGTGGATTAATACCACTTTTTTATTTATAAAAGAAGGAGGAGTTATTATTAATAACCAAAAAATTGGAGACGATTTAAATAACAATCAAACAAATCGTAAACCAAAGTCAGAACATGTTATCAACAATGCTATTCCATACAAAAAAGTTTTTGTTCTAGGACCTACAGGGGAAAAAATTGGTGTGTTAACCAAAGAAGAAGCTTTAGAAAAAGCATCTGAATATAATATGGATTTAGTTTTAATTGCAATAGAAAACAATAAGCCAATAGTTAGAATATTAGATTATGGCAAATTTAAATATGATAAGAAGAAACGCCAAAAAGAAGTTAAAGAAAAACAAGCAGTTACTGTTAACAGAGAAATTCGCTTAACTCCATTGATTGGTGATCATGATTTGAAAACGAAGGCAAACAAAACTAGAGAATTTATTTTAGATGGTAATAGAGTTAAAGTTTCAGTTAAGTTCCGTGGTAGAGAAAGAGCAAGAATTGAATTAGGGGAAGAAATATTAAAAAAGTTCTTTGCCTTAATTGAAGATGTTGCAAAAATTTCAAAAGAAGCAACATTAGTTAATGATAGATTTTTAGATATGTATGTAGAAAAAGATAAACGAAAAGTTGATACATTAACCAATAAAAAAGATAACTAAACCTTCAATAGCATAAGGAGAATAAAATGCCAAAAATGAAAACCAAAAGCGGTTTGAAAAAAAGAATTTCAATCACTGCATCAGGAAAAGTAAAACGTGGACAAGCGTTTAGATCACATTTAGCACAAAACAAAACAACAAAACAAAAACGTCACGCTCGTAAAGCAACAACTTTATCAAGTTCAGATTTTAAAAGATATAAAGAATTAATTTAGAAAGGATAAATTATGCGTACTAGAGGCGGAATTGTAACAAGAAGAAGACGTGCAAAATGAATAAAATTAGCTAAAGGATATTGAGGACACAAATCAATAGGATATAAAGTAGCTAAACAAGCAGTTGTTAAATCATGGACATATGCATTTAGAGACAGAAAACAAGTTAAACGTGAATTCAGAAAATTATGAATTGCTCGTATTAACGCAGCTGCAAGACCACTAGGAATTACCTATTCAAGATTAATTGAAGGTCTTAAAGAAGCTAACGTGTTAGTAAATAGAAAAATGTTATCTGAATTAGCAATTAATTATCCTACATCATTTGCTAAATTAGTAGATCAAGCAAAATCAGCTTTAGCAAAAAAATAAATCTGTAATGGATTTATTTTTTATTATATTTATTAATAATTTATTAATAAATTAATTAAGTTCTTTATAATTTTATTTATATTGCGTAATGGAGGAAGATGAAAAAAAGAGTGGTATTAGGAATGAGTGGCGGAGTTGATTCTAGTGTTTGTGGATATTTGTTGCAAAAACAAGGATATGACGTTGTTGGTTTGTTCATGAGAAATTGAGATTCATTTTTAAACAATGATATTTTAGGAAATGAAAATATAACTCAAAATATTTGCCCACAAGAGCAAGATTATTTAGATGCAAAAACGGTTGCAAGCCAATTAAATATTCAATTATATAGAATTGATTTTGTTAAGGAATATTGAGAAAATGTATTCAATTACCTAATTGATGAATATAAAAAAGGTAGAACTCCCAACCCTGATATTTTTTGTAATAAATTTATAAAATTTAAAGCTTTTTCTGATTATGCCTTTAAAGAATTAAAAGCCGATTATATTGCTATGGGGCATTATGCAAATGCAATTGACGGAGAATTATTTCGGGCGCAAGATGATAATAAAGACCAATCTTACTTTTTAGCGCAATTAAGCCCTGGCCAACTTAAAAAAGTAATTTTTCCATTGGCTAATATTACCAAACCAGAAGTAAGAGAAATTGCAAAACAATTAAACTTGTCAACTGCTATGAAAAAAGATTCAACAGGAATTTGCTTTATAGGTGAAAGACACTTCACAAAATTTTTAGAAAATTATATTCCTGCTCAACCAGGTAATATTATTGATATATTTAGCGGGAAAGTGGTTGGAAAACATATTGGTGCAATGTATTACACTATAGGCCAAAGAAAAGGTTTGAATCTTGGCGGGTATGAAAAACCATATTTTGTAGTAGGACATAATCTTTCAAAAAAAGAAATTTATGTAACAAACGATGACCATATTGACTATTTAATGTCAAATAAATTAATTGCAACAAATTTTAATCAAATTTCAAAAGAATTTAATTTTGCTAATTTAACTGCTAAGTTTCGTTATAGACAAAAGGATATCAAAATAACTTTAAAGATTTTAGATGACAATAAAATAGAGGTTTGCTATCCAAATACCGAAGCAGTAACCCCTGGCCAACAAGTTGTAATATATGATGGAAATAAAGTCGTTGGTGGAGCAATAATTGACAAAATTTATTTAAATAATGAATTAAAAACGTTTTTATAAAGGAAAGGAAAATAGAATGCTAACTTCAAAAGAAATTAGAAAAATGTGATTAGATTTCTTTAAATCTAAAGGACATTTAGTGGTACCTTCAAAATCTTTGATACCACAAAATGACCCTTCTTTGTTATGAATTAACTCAGGGGTTGCAACATTAAAAGATTATTTTAGTGGTAAAAAAATACCTCCTGCTAAAAGAATTACCAATTCTCAAAAATCAATTAGAACAAATGACATTGAAAATGTAGGAATAACAGCAAGACACCATACATTTTTTGAAATGCTTGGTAACTTTTCAATAGGCGATTATTTTAAAAAAGAAGCAATTGCTTTTGGATATGAATTTATTTTTAATGTTCTAAAACTAAATAAACAAAATATTTATATAACTTATTATTCAGAAGATAAAGACACTTATAATTATTGAATTTCAAATGGTATTGATCCTTCGCATATGATAAAAGGTAGTAGAGATACAAACTTCTGAGATGTTGGCCAAGGACCATGTGGCCCTGATACCGAAATTTTTTACGACCGTGGGACAAAGTATGATACCAGTGGCATTGAATTGCTAGAAAACGATATTGAAAATGATAGATATATTGAAATTTGAAATATCGTATTTTCACAATTTAATAATGATGGTGAAAACAATTACACCGAATTAGCAACAAAGAATATTGATACTGGCGCCGGACTTGAAAGAATAACATCAATCTTGCAAGATGCTCCAACTAATTTTGATACTGACTTATTTTTACCAATAATTCATGCGACAGAAAAATTAACAAACAAACGTTATGATATTAATAACTATTTTACAAAAGAACCAATTCAAATGAAAATTAACACCCAATTTAAAATAATTGCTGATCACATTAGAGCTTCAGTTAATGCAATAAGTGACGGTGTTAAACCTTCAAATATTGGTCGTGGTTATATTATTAGACGTCTAATAAGAAGAGCATATAGGGCGGGTATTTCTCTTGGCATAACTCAAAAAGCATTTTTATATGAACTTGTTCAAATAGTTAAAGATTCATTAATTTATGACATTGATGTTGAAGAAGTTAAAAAAGTTATTTTGGATGAAGAAAATCTTTTTGCTAAGACTATTAATGAAGGCGAAAAACTATTAAATCAAGAAATTGAAAAAAATGGTACTATCGATGTTAAAATAGCCTTTAAAATGTTTGACACATATGGCTTTCCTATTGAACTAACAAGAGAAATTCTGCAAGAAAAAAACATTGACATCGATATGAATGAATTTAATGAATTTTTAAAGCAACACCAAGAAAAATCAAGAGGAAATATTGAAGCTGGAATGCAAAAGGTTATTAATTCACTTTCATTAATAACTGATAAGATATCTGAATTTGTGGGATATGATTACACTAAAACAACTTCAAAAATTTTATATTTATTAAATAGTGAAGAAAAAATAAATAATGCGGGCGAAGATGAAGTTTCTTATTTAATATTAGATAAAACTCCTTTCTATGCTACAAGTGGTGGCCAAAATCATGATGAAGGTTACATTTTACAAGGGAATAATAGAATTGAAGTTTTAGATGTATTTAAAGACAAATATTGAAATCATGTTCATGTTGTAAAAGGCAAAATAAATTCAAATGAACCAGTTCAATGTTTTGTTGATGAAGATAAACGTATTAATTTTGCAAGAAATCACTCTTCAACTCACTTGATGTTTAGTGCATTAAGAGAAACATATCCAAACGAAATTATCAAACAATTAGGTTCGAATATTACATATGAACATTTTACATTTGATTTCGGTCTTGATCATAGGCCAACTAAAGAAGAGATAAAATTAATTGAACATAAAATGAGGCAATATATTGCTAGAGATGTAAAACGTAATTACATAATAACTTCGCTTAAAAAAGCAGAAGAAATGGGCGCATACATGACAATTGATGAATCAGAATATCATGATGCAAATTATATAAGAGTTGTTGAATTTGAAGGTATTACCAAAGATTTATGTGGTGGAACACACGTTCCTCATTCAGGTATAATTGAAGATTTTAAAATTGTTGCCGTTGATTCAAAAGGTACAGGAATATATAGACTTAGAGTAGTAACTTCATATGATTTGGTAAGAAAATATTTATTATCAGAAATTCAAGAAAAACAAAAAATATTGGATACATTAATTAAAAATAATCAAAAACTAAATGAAGGCTATAACTTTAAATATACAAAGGAAATTATTTCTAATAAGACATCTGATTTAGAAGAATTACTTGAAGAATATGATAAGTGCGAAGAACAATTAAGAAACGATTATCGAGCATTATTAAAACAAAAACAATCAGAAGATGTTGATTTAAGTTTGTATAATTATGAAACCATCAACGATAAAAAAGTATTCTTATTAATTAACATAGAACAATCATTAATTAAAAAAGTTTCAATCTCATTAAGAGAAGCAAATCCTGATTCAATTATTTTTATCTTAAACAAGAGCAAACAAGATTCATATTATTTTGCAGTAGCATCTAAAAAATATGATTCAAATGCAATATTTAAAAACTTGGGCAAGGTTTTAAATATTCATGGCGGTGGAAATGCAATAATTTCTCAAGGTTCATTGGTTTCAAACTTGAAGCAAGAAGAAATTATTGAAATTATTAAGAGCAATTTATGCGAATTTTAGCATTGGATTTAGGCACAAAAAGTTGTGGCTTTGCAATAAGTGATCCTAATAATATAATTGCAAGTGGACTTGAAAATTTTAGATTTGAATTGAACCATTTTAACTTAGTTATTGAAAAAGTGTTACATTATTTAAACAAAAGTGAATATCAAGGTTCAATTGGCACAATAGTTTTAGGATATCCCTTAAGAATGGATTTATCAAAAAGTCAACGAACGTATATGGTCGAAAGATTTGGAGAAAGATTAAAAAAAGTTGTTGATATTCCAATTGTATTTCAAGATGAAAGACAAAGCACTATTGGAGCTCAAGAAATATTGCAGAGCGCTGGATGAAACACTAAAAAATCTAAATCAAAGAAAGATTCACTAGCAGCGCAACTTATATTAGAAGATTATTTAAAAGGAATTAAATATGAATAAAAATATTATTAATTTAGATGTAGTTGATAGGCAATTGACTACAAGCGATGGCGAAAAACTTTATGTTATTTTTGATATTGAAGAAAACGGCGAACATTATCTTGTTTTAACAGATTATGACGCGATTATTTTTGCTAAAGAACAAGATCAAAATTTAATCGAAGTAACAGATGAAGGTGAAATTGACATTCTTGTTGATTTGACGATGGAATTTGCAGAAAATAATTTTGTTTTAGATAAAGATGGAAAATCAGATTTAATGAAGAAATTAATTGGCAACGATCAAGGTGAAAATGAAGCATAAGGGTTCAATATTTAGCGCTAATACAAAATTAGGTTTAATAATCTCTGTCGTCGTTGTATTTATTTTAATGTGTGTAATTTTGTTAATAACCATTTTAGCACGTAGACATTTCTTAAAAAGCACAATAACATCAAGCGAAGAACGAGAACTACAAAATTTAAAAACAAAAAATCCAAATTATGGAATTGTTTTGAGTGGCATTAAAAAATATTATGCTAATGTTTTAGGCGACCATTTGATTGCATTTTTAATTAATACAATATATTTGAATAATTTTTTAAACGTTAGTCTTATTGAAAAAAATGATTATGCTTCAATTTCAATAGCAGCATTATCTATGGCTAATGTTTATTCAAAAAATCAATATTTAATTCAAAAGCAAGGTTCAAATATTCAAAACGAAAATCAAGATTTAGATTTATCTAGATTAAATTATGCTGATGAAATTTATAATCCTTCAGATATGATAATATCTTTATGTGATGATCTTTCAATAGAACAACAAATTAATTCAAATTTACCTTATTTAAGTGATAGAGGTATGCTAATTGTTTCATTTAAGACTATAAAAGACTTTAAAAATCAAAAAAATGTTATTTTAGATAATAATTTAAGATACGAAACTGTTAAAATTAGTAATAAAAATGTTATACTTTTAGCAAAAAACAATATGAATTCAAACAATGAAAATTCTAAGGAGCAACAATAATGGCTAATAATGATAATAAGATTTATTTAACAGAAGAAAGTTTAAAGCAATATCAAGAAAAATTAAAATATTTACAAGAAGTTGCAAGACCTCAAGTTATTGAAGAAATTAAAGAAGCAAGAAATCAAGGTGACTTGTCAGAAAATGCTGAATATGACTCAGCTCGTGATAAACAAGCTGCTATTGAAAATGAAATTTTAGAAATTCAACATATTCTTGAAAATGTTGAAATTATTAAATCAACATCAACTCATACAATTAGAATTGGTTCAACTGTAAAATTAATAAATATAAACACAAACGAAATAAGCGAAGTTAAAATTGTTGGTGCATTAGATGCCGATCCATTCAACAATAAAATTTCAAATACCTCACCGCTTGCAATTGCATTAAATGGCAAAAAAGTCGGACAAGTGATTGAAGTTGAAGCTCCTAAAAAATATAAAGTTAAAATTGAATCAATTTTATAATTTAAAAATATTTGCACTGTGCAAATATTTTTTATTGTTATTTTATAAAATAAAATATTTTATTTAATGAAACAATTTCACACATTAATTTATAATATCTAATAATGAAAAATGAAGTTGTTAAAAATAATTTAGGATATAGCGGAGATTTATTTGTATATCAAGATAAATCAATGTTTAATTACTCTGTTGATACTATTTTACTCGGCAATTTTGTATCAATAAACAGAAAAGTAAGAAATATTTTGGAAGTAGGCACGAATAATGGTGCCCTTTCTATTTTTATTGCTGCAAGAGATAAACGTTTGAACATTGATGCTATTGAAATCCAAAGCAAAGCTGTTGAGCTTGCAAGAAAAAATATTATTTTAAACAAACTTGAAAATCAAATTAATCTTATTGAAGGCGATTTTCGGATGTGGGCTAATCAAAAGGCATACGACTGTGGTAATAAATTAGCCAAAAAATACGATTTAATTGTTGCTAATCCGCCTTACTACAATGAAGATTATAATCAACAAAGAAAAAACTCAACTCTTGAACAAAAACTAGCAACTCATGAAATAAATTTAAATTTAGAACAATTAGTTGCTAATAGTGCAAGAATTATTGAACAAAAAGGATACTTAGCAATTGTTTTGCCAATGGCAAGATATATTGATCTAATTTGTTTGCTAAGAAAATACAATTTTGAACCAAAAAGAATTCAATTAGTTTATCCCAGAATCACTGATTTACCAAAATTCTGTTTAGTTGAAGCTAGGTTTAATTCAGGTTGAGGAACATATTTTGAAAGTAATATTTATTTACATAATGAAGATAAAAATGATCACAGTTATAGCCAAGAAGTAAAAGAATTATATAAACCAAAAAAATCAAATTTTTAAATATAAACATTGAAAGGAAAATATGAATAATAATAAAAAGACATTTTATATTACCACACCAATTTATTATCCAAGTGGTAATCTTCACATAGGACATTTATTGACTACTACATTAGCTTCAATATATAGAAATTACAAAAATCAACAAGGATATGAAACCTTGTTTATTACTGGAATTGATGAACATGGTCAAAAGATTCAAAAAAAAGCTCTTGAACAAAATATTACTCCAAAAGAATACGTAGATATTCAATCTCAAAAGTTCGTTGATTTATGAAAATTATTAAAAATTGATTATGATAAATATATTCGTACAACTGACGATTATCATATTGAAACAATTCAAAAAGTTTTTTATTTAATGGAACAAAAAGGCTATATTTATAAATCTAAATATGAAGGCTTATATTCAATTAGCGATGAAGAATTCTTAACTAAAACTCAAGCAATATCGAAGGATGGAAAATTTTACCACCCAATTTCAGGACACGAACTTCAAGAAGTAAAAGAAGAAAGTTATTTCTTTAATATGTCAAAATTTACCCCTTGAATAAAGTCATATTTCGAACAAAATCCTGATTTCATTACCAATAAAGCAACATATAAAGAATTAATGAATAATTTTATTAATAAAGGATTAGAGGATCTTTCAATAACAAGAGTTTCTTTTGACTGGGGAATTAAAATAGAAACTAAAGATGCTGAAAAGCAACACATTATTTATGTTTGATTAGATGCTTTATTTAACTATTTATCAGCATTGGGATACAACAATTACCTAGGCCAAGAGTTATATAATAAATTTTGGGTAAATGGTAATGAAAGAGTTCATTTATTAGCTAAAGAAATTTCTAGATTTCATGCGATATATTGACCTATATTTTTAAAATCTTTGGATCTTAATTTACCAACAAAATTAGTAATTCACTCATGAATTGTAACTCCTGAAGGAAAAATGTCAAAATCAAAAGGCAATGTTATTGAGCCAATTCCTTTAATTGAAAAATATGGGGCAGAACCCGTTAAGTATTTTTTTGCATCGCAAATAAATATTGACAATGATTTTAGTTTTAGCGAAGAATTACTTATTAATGTATTAAATGCTGATTTAGCAAATAATTTTGGTAATTTAGCCAATAGAACCTTTAAAATGGTAATGTTAAATTTTGAAAATGGTACAACTTATTGTTCAAACCAATTGAATTCATTAGATAAAAATGTTTATTTAGAAATAGAAAATGCATACAATGATTATAAAAAATATTTTGACAATTTTAATGCTGATAAGGCTTTAAAAAGGGCAATTGAATTAAGTAAGTTTTTAAATGAATATATTGATAAAAATGAACCTTGAAAATTAAAAGAAAATTTGCCAAGATTAAATGTGGTTTTAAATACATTGTTGAATGGAATTTATGCAGTTAATATGATGTTAAGCGTTGTTTTAAAAGAAAAATGTAACATTTTATTAAATGATTTGCAATTGTCTGAATTCTCTGAAAAAATGTTGTTTAATTATTCTAAATTTGATAACCAAAAGTTTTCTAAACCTAATATTTTATTTGAAAGGATAAAACCAAATAATTAAATTATTGGTAAAATTAGTTCATGAAATTTATAGATGAAACAAATGTAATAGTAATAGCTGGCAAAGGTGGCGATGGCATAATTAGTTTCAGAAGAGAAGCTAATGTTGACAAAGGCGGTCCTGATGGTGGCGACGGTGGCGACGGTGGCGATGTTTATTTTGTAGGTGATCCTGGCCAAAATACACTTCTACCATTTTATTATCAAAATAAATTGGTCGCTGAATCAGGAGAAAATGGAAGGCCAAAAAATCAATATGGGGCTGATGGCCAAGATTTAATTGTAAAAGTTCCACTTGGAACAATGGTTTATAATGGCGATGATTTAATAGCCGATGTAGTTAGCCAAGAGAAATACTTAATAGCAAAAGGTGGACACGGTGGCAAAGGAAATCCTAGATTTAAATCTAGTACTAACACTGCTCCTAGAATATGTGAAAATGGTACACCTGGTCAAAAATTTGAACTTCACTTAGTTTTAAAAGTTTTAGCAGATGTTGGCTTTGTTGGAAAGCCTAGTGCTGGCAAGAGCACTATTTTAGGCGAAATATCTAATGCAAAGCCTAAAATTGCAGATTATGATTTTACTACATTGGTTCCTCAACTAGGTTTGGTAAAATATTTTGACAACTCATACGTTGTAGCCGATTTACCAGGACTTATTGAAAATGCTCATTTGGGCAAGGGCCTTGGAATTCAATTTTTAAAGCATATTGAAAGATGTAAAATTATTGCTCATGTTATTGATTTTGGTAGCGAAGAAAAGAATCCAATAGATGATTATGAAATAATTAACAATGAATTAAAATCATATAATTTGTTGCTTGAAAAATTACCACAAGTAGTAATAGCAAACAAAAAAGATCTACCGGCTTTTTCACTAAACTTATCAAAATTTAAAGAAAAATATCCAAATATAAAAGTATTTGAATATTCAGCATTAATGGCTGAAAATTTAGATGAATTAAAAGGCTGCTTGTTTGAAACATTGCAATCTGCTAAAGATTCAATCGCTGTTCAACAAATTCATGAAAGTCAAAACATTGTAAAAATAACATTAAATAAAAAGCCTATTCAAATTAAAAAAATTGATAATGAAACATATGAATTGTTTGGTGATGATATAGATAATATATATAATAAAATACCAGTGATTTCATTAGATAATTTATGAAGATTTAATGCCAAATTAAAAGCATTAGGTGTATTTGAGTTAATAAAACAAAATAATATTAAAGATGGTTCTACAATAAAAATTCATGATTATGAATTTATTTGGAATGCTGAAGAATTTTAAAATTTGTCAAGATTTTATTATTTTTTAAAAATTGTATATTACTATGTAATAAAAAAAATTTTAATTTTAAAACTTTTAAAAAAATAATATATAATAATTAAGCATTAAGCAAAAGCAATAAGTATTTATTTATAAACAACAATTGTGGGGAAGTAGCTCAGCTTGGTAGAGCACTTGACTTGGGCTCAAGCGGTCGCAGGTTCGAATCCTGTCTTCCTCACCATTTATGGGGGGGTAGCTCACCTGGCCAGAGCGCCTGCCTTGCACGCAGGAGGTAGAGGGTTCGAGTCCCTTCCTCTCCACCATTATGGCACTGTAGCTCAGTCGGTTAGAGCGTCCGGTTCATACCCGGAAGGTCAAGAGTTCGATTCTCTTCGGTGCTACCAAATGCAACAAGCCTAACGGGCTACGGACCCATAGCTCAATGGTTAGAGCAACCGGCTCATAACCGGTCGGTTACAGGTTCGAGTCCTGTTGGGTCCACCATTGGGCAATTACCCAAGAGGCTGAAGGGAGCAGTCTTGAAAACTGCCAGGGGCTTTACGGCCCGCGGGGGTTCAAATCCCTCATTGCCCGCCATTATATAGCGGAGTAGAGCAGGCGGCAGCTCGTTGGGCTCATAACCCAAAGGTCATTGGTTCAAATCCAATCTCCGCAACCATGGTCCAGTGGTAAAGTGGTTTAATACGCTTCCCTGTCACGGAAGAGATCGCGAGTTCAATTCTCGTCTGGACCGCCATGGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCACTGGTTCGATTCCTGTCGGAGCCACCATTTCAGAGATGACTAACACCTTAGGGTGTTTTTTTATTTTTTATGTTTAATAAAAGATAAAAAAAGTAAAGTATAATAATACATGTTATGAATATTAATAGTATAAAAATTGCAATTCAATTATTTGGAATTTTTGGTTCAATTGTAACGATTTGTTTAGGAATTCCACAATTAATTAGATTAAAAAAAACAAAAAACACGGGCAAAGTTAGTTTTGCAGCATTTTGAGTTTTTTATTATGGAATTTTAGCGTGACTAGTTTTAGGAGTATTTACTTCTAGCGAAAATTCATGATATTTATTCTTATCAAACTTTGCATGTGTTACAATAACATCCTTTACAGTATTTTACTTGTATTGATATTATGAAAAATTAACTAAAAAAATGTTAATAAGGGCTATTGTTGGAATAAGCATTAATCAAGTTATTTCATTAATTTTCTTGTGCCTATTTTTTGTTACGGTATATCAAAAGTTGAAATTTGGTAAATTTTATCCCGATAATTCAATTATTCCCGTTCTTCCGGGTAATGCACAACTTGCATTTGGACTAATATGTCCCGCCTTTACAAGTGCAGCATTTTTCCCTGGATTAATTGTTAGTTTAATTAAAAAAGAAGAAATAAGTTTAAGCCCATGAATGGCTCTTGTTTATATGCTAAATAATACTTTTTGAATATTGTATTTCTCATTAAATATTGTTTATTCGAAGTTTGGCGTGGATCAAGCAGCTCTTAATAGTATGCCAGGATTTATCGGGGGACTAGTTTGACAATTACTAGCATTAGGAATGTTTGCTTTCCAATTTGGAGTTACATTACACTTTGATATTAAGCACAAAAAACAACAAAAGCAAGAAATGGTTAACGTTATCAATCAAAATTAATCAAATTAAAAGTAGCAAATTACTGATTGCTACTTTTAATTTCATCATTTGCAACTTTAGAATATGCTCTAATTAATTTACTTGCGCCTAATTTTATGCCACCAAAATATCTAACTACAAACAACACACAATTATTTAATTTATTTCTTTCTAGTAAAAAATATAAAGGTTTGCCTGCTGTTCCTGATGGTTCACCATCATCACTCATTCCACCACATTGAACGCCATTTTCAATAACTAAATAAGCATAAACTATATGTCTAGCTTTTTGATGTTCACTTTTTAATTGCTCTTTTAAAAGTTTCACATCATCTTTACTCTTGATATTAAATTTATAAGAAATAAATTTAGACTTTTTAATTTCTAAAATTGCCATAGTTTTATTATAATAAAAATTATTGTATATAATTACTAACATGTTAAAAATTATTTCAAATTATTCAGATAGAATAGATAAATATGTTGCTAATAATAGTGAGATTTCAAGAAACGATATTAAGGAATTGATTGAAAATGGCGCAGTATTTGTAAATTCAATAAAGATAAACAAACCTAAATTCGTTGTTAAAGAAGGCGATGAAATTTTAATTGAAAGATTATTAGATAAACAAATAAAGGTTGAACCTCAAAACATTCATTTAGATATAGTGTATGAATGCGATGATTATTTAATAATTAATAAGCCATCAGGCTTAGTTGTTCACCCAGCTCCTGGGCATCATGACAATACATTAGTCAATGCTTTGATGTATCATTTTAAAAATAATTTAAGTGATGTTAATGGTTTATTACGTATGGGGGTTGTTCATAGAATCGATAAAGATACTAGCGGCTTATTATTAATTGCTAAAAACAATAAAGCTCATAACTACTTTGCTTCCCTTTTAAAAACACATGAAATTAAAAGGAGCTATTATGCTATTGTTGAAGGCAAGATTGCAAATGAAGAATTACATATTGAACTTCCAATAGGTAGAGATACTAAAAATCGCCAAAAATATGCTGTTAGTGAAACAAATTCAAAACCCGCTTATACAATAGTAAATGTTGTCAAATATTTTAAAATGAATAATAAAGATTTTTCGTTAGTTAAATGTAATTTAAAAACTGGTCGTACTCACCAAATTAGAGTTCACTTAGCATATATTAAACATCCTGTTTATGGAGATCCAATTTACGGTCACAAAATTGATGATTTCAATCAAAGGCTTCATGCATTTGCTTTAGATTTTGTTGATTTAAATGGAAACAATAAACATTTTGAAGCAGAAATGCCTGAAATTATGAAAAAAGAAATAACTGGCGAATAAAAAAACCTAAATTAAATTGCAATTTAAAATATAATTATTAAATATAAAAGGAGTTTTAATGAAATCAATAAACATAAATGATTATAATTCTACAAATGAATTATATACAAAGAACAAAAAAGTTTTTTGAGCTTGATTCATTGCATGAAGCGTTTTATTTGCAACAATTTTAATATTTGTAATTGCTTCTTTTATTCAATTTGCAGTTGAAAAACAAAACTATTTGAATAAATATGCTGAACTTTTTGGCCAAATCCAAAAATTAAATCCAAATTTTCAAGGAGATGTATTCGCACTAGCCAATAATGAATATGTCAAATCTTTGGCAATGTCAGGCGTTTTACTTGTAATTTTAATTGCGGTTTATGTTTGACATATTGTTTCCATAGCATTGGTAGCCAAAAACAAAGATTTTTCTGCTTATTCAAGAATTTTAACTACATTATATTTATTTGTAATTATTTATGAAATAATAACATTAATCTTTTCTGGATTTCAATCATTTTCGTTAGCAATTTGACAAGTAAATAAAATTTTAAATTTAGTGGCAACATTGTTATATATAGTTTCATATTTTGCTGTATTTTTAAAATGCTCTTATGTCGCAAAACTATTTTTAATTTTAAAGAGAAATTTAGCATTTAAGGCATTAAACAACTCGGGCTCAATGGATGATATTTTAAGATCATATTTCTATGGTCAAGAATCTCCTGAACCAACACTAAATACCAATAATACATTTGATTCAAATCAAAAAACCGAAGAAAACAAATCTGAAGATTTAAACAAAGAAGAAGATTCAAAATCAATTGAATATCGTAGTAAATTAGAACAATTAAATGATGAACAATTGCAAAATATGGCTAAAAAATTAAATATTTTTGGAATTGAAACATTTTCAAGAGAACAATTGATTTCAAAAATTAGCGATATTTTTGAACAATCAGCCAAGTCTCAAAATCAACAATCAAAACCAGATAATTCCGATGAAGACAACAACGGCGGAAGTCAAACCAGCAATTAATTTTTGTAATTTTATAATTACTATTTAACAAGGAAAAAACAATGAATAATCAAACATCATTAAAACAACATATTTTTACTTTTTTTTCACAAAATCCTCCTGCTAGTTCAATAAAAAATGGTGATCAAAAATTATGAATTCCTGCATTAGTTTGCATTATACTAATTTTATTTTCTCTATATCTAGCAATAAAATTTTTTGTTAATTTTAAACGTAGCATTGCTAAAACTAAATCTATTTTAAAAGACGATCTTGTTAGTGCTTATGTACAAGGCTTTAATATTAAATCCTCAGCAGTTTTTAATTTTATATTAGCTAACATAATTTCTTTAGTTTCAATTATCATTTCTATAATATCAATAGTTAAACATTTTTCGGCAAGTTCAACGTTAGGTAAGTCTTCGTTTCTAGCGTTGTTTATAGCAGTGATTGCGCTTTCAATTGTTTTAATAATATCAATTAATTTATCACTATTAGCAATTTATTTTGTTTCATTTAAAGAAGCTAAGTTTGTTAATAAAAAAAATTTAGAAGATGAGCTTATTTCTTTGAAGTATAAAAAATCCAAAGAATTAAATGATTTAAATGCCCCTGAAAATATTAAACTGGATATCAAAGCAATGGAATCACACAACCCATTAGCCCCTAGAGTGTTAGCAAAATTTATTGATTTTTATAACAAAATGTCAAGTATTGAATTATTTAAGCGTTATAAGAATTATTTAAATCAAACATTCAAAATTAGATATTTTAATGAATCTCTTGAAGTAATTGAAGGAAAAAATGAAATGGAAGATAATCTTGAAAAATATCTAGACAACATAAATAATAAAGATCCTGAATCACAAAAAAATTCTCTAATTTTAAAAGAAATTGCTTGAATGAATGATATGGATAAAAAGGTTAAAATTATTAGAGAAGCAGATAAAAATATAACCATGTCTAAAAAAGAATTTCAAGAAAAATATGATGAATATGTTTATTTGCAAAGATCACAAGATCCTGCATATCAACAAACTCAAAAGAATACATGACTTACATATCGTGATTCTGATATTGAAGATCTATTTTATAACACAAATACATCAGTTATTTATACCATTGATAACGGCAAGACAATTTTAGAAAAACCAATGAATGAATTTTTAAATGAATATGGACAATATTTAGAAACTAAATTTTTTGCATCAATTAAAGAAAATGAAGAACTTAAATAAGAGATTGTAATGACTAAGAAACACAAAAGAATTTTATTTTTATCAACATTAGCATTACCCGTAGCCCTTGTGCCAGCAACTATGGTTTCATGTTCAAAAAAAGATATTGAAGAAATAAAATTGGATATGATCACAATAGCAAAAAATCAAATGCTAAATTATTTACAAAAATTTGAATCATTTTTTAAAAATGATGATGATCCTGATGTTAGAAATTTAGAAGTAGAATTTAATAAAATTATAATTTCATTAAAAAAAATGGATATAACACCTGATTTTAATATGGATCAATTTATTCAAGAAATGACTAAAAAATTTAGCGCTGTAGCAAATAATTTTTTGAATATAAAATACAATAAGGCCAAATTAACAAGTGAATATTATATTTCAAAAGTAAAAGCTCAGCTAATTTTGGAGCAATTTAAAAATTCTGGTATTCTTCCAGAAAAAAATGAATTTGCCAAGATTTTAGAGCAAGAATTATCAAAAGTTTCCGATCCGACTATTAAAACCTCTGAAGAAGAGTTAAAAAAATCAATTAGATTTTTAGATGATTTAAACAAAAAATATAATGAAATTTTAAAAAAGCAAGTAATGATTTCAAAATAAAATTTCTTGCTTTTTTTATTTTTTGTTTTTATTTAAAAAAGTTTAATTTTTTTTAAAAAACACCGGGTTAGTTGTTGTATAATTGTACAGTATTTATCTATTAAAATACCAAAGACCATATAAAAATACAATATATAATTTAAATTTTAGAAAGGAAAAAATATGCCTACAGTTGCACAATTAGTAAAACAAGGCCGTAAAGATAAAACAACAAAATCTAAAGCTCCTGCTTTAGGAAAAATGTATAACTCTTTACAAAAAAAAGAAACTTCTATGCCCGCACCATTTAAGCGTGGCGTATGTACTCGTGTGGCAACAATGACACCTAAGAAACCTAACTCAGCTATTAGAAAATATGCCCGTGTTAGATTATCAAATGGTCAAGAAGTTACTGCCTATATTCCCGGTGAAGGACACAATTTACAAGAACACAGTGTTGTTTTAATTCGTGGAGGTAAGGTTAAAGATTTACCCGGAGTTAGATACACAATTGTTCGTGGAACACAAGATGCTGCCGGAGTTAATAACAGAAAGCAAGCTCGTTCTATTTATGGAACTAAAAAACCAAAAAGTAATTAATTATAGTTAGAAATTAGAAAGGAAAATCATATGTCAAGAAAACATAAAGCGCCTGTTAGAGATGTCTTAGCAGATCCAGTTTTTAACTCTAAGATAATTACTAAATTAGTAAATACCATTATGTTAGATGGTAAAAAATCTATTGCTGAAAACATTTTATACAGTGCATTTGATATTATTAAAACAAAAACAGGAAAAGAACCAGTTGAAGTATTTAATCTAGCATTAGAAAACGTTAGTCCACAACTAGAAGTTAGATCAAGAAGAGTTGGTGGTTCAAACTACCAAGTTCCTTGTGAAGTTTCTGCAAAAAGAAAACAAACTTTAGCATTAAGATGAATTATTCAATATGCAAGATTAAGAAACGATAAAACTATGGAAGAAAAGTTAGCAAATGAATTAATAGATGCCTCAAACAAAGTTGGTGGCGCTATTAAGAAACGTGAAGATACCCATAAAATGGCTGAATCTAACAAAGCATTTGCTCACTTTAGATGATAATAGGTTGGTTGTATGTCTCGTGAATATAAATTAGAAGATTACCGCAATATCGGAATCATGGCTCACATTGATGCCGGAAAAACCACAACTACTGAACGTGTGTTATATCACACAGGAAAAATTCATAAAATTGGTGAAACACACGAAGGTGCTTCACAAATGGACTGAATGGTTCAAGAACAAGAAAGAGGTATAACTATTACCTCAGCTGCTACTACAGCTTATTGAAAAGGAAAAAGATTAAACATCATCGATACCCCAGGACACGTTGATTTTACTATCGAAGTTGAACGTTCGTTAAGAGTATTAGATGGTGCAGTTGCTGTTTTGGATGCCCAAAGTGGTGTTGAACCTCAAACAGAAACAGTTTGAAGACAAGCAACAAATTATCATGTTCCAAGAATAGTTTATGTTAATAAAATGGACAAAATGGGTGCTAACTTTAAAGCATCTGTTGAATCACTTCATAAATTACTTGGTGCTAATGCTCACGCTATTCAATTAAATATTGGTGAAGAAAACCATTTTGTTGGAATCGTTGATTTAGTTGAAATGAAAGCATATTCATATGATGGCGAAGTTGATGAAAACGCTAAAGAAATTGCTATTCCAGAACATTTAAAAGATGAAGCTCATATTATGAGAGCTGAATTAGCTGAATCACTTGTTGATTATGATGAAGAACTTATGACTTTATTATTAGAAGAAAAAGAAGTTCCAGTTGACATTTTAAAAAGAGCAATTAGAACTGCTACAATTTCTTCTGAATATTTCCCAGTTGTTTGTGGAACATCATTTAAAAACAAAGGTGTTAAGTTAATGCTTGATGCAATTGTTGATTATTTACCAAGTCCTTTAGATATTCCTCCAATGAAGGCATATAAAGGCGAATCAGAAATTCATATCCCTGCCTCAGATGACGAATTTTTCTCAGCTTTAGCATTCAAAGTTATGAATGACCCATTTGTTGGAAACCTAACATACTTTAGAGTTTACTCAGGAATTGTTTCAAAAGGTTCTTATGTATTCAATTCAACAAAAGGTGAAAAAGAAAGACTAAGTAGAATTCTATTGATGCATGCTAATAGTCGTACAGACATTGACGAAGTTAGAACAGGAGATATTGCTGCAGCAGTTGGTTTGAAAGTAACAACCACTGGAGATACATTGATTGATGAAAAACAAAAAGATGTTGTATTGGAAAATATGAATTTCCCTGAACCAGTTATATCTCAAGCTATTGAACCAAAGACCAAAGATGCTTCTGAAAAATTATCATTAGCATTGCAAAGATTGGCTGCTGAAGACCCAACATTTAAATACTACACCGATGAAGAAACAGGACAAACAATTATTGCAGGTATGGGTGAACTTCACCTTGACATTATTGTTGACCGTCTAAGACGTGAATTTAAAGTTGAAGTAACAGTTGGCGCACCACAAGTTTCATATCGTGAAACTATTACAAAGACTGCCGATGTTGAAGGTATGCACAAAAAGCAATCTGGTGGTAAAGGTCAATATGGACACGTTTGAATTAAATATGAACCAAACCCAGATGGAGGATTTGTATTTATTGACAAAATCGTTGGTGGTAAGATTCCTAAAGAATATATAAAATCTATTGAAAAGGGACTACGCGAAAAAATGGCTATTGGTATTTTAGCAGGATACCCTATGATAGATATCAAAGCTACTTTATTTGATGGATCATACCACGAAGTTGACTCGTCTGAATTAGCATATAAAATTGCTGCTTCTAAATCACTTACAAAAGGCCGTGAAGCCTTGGGAACAGTATTACTAGAACCTATTATGGATGTTGCTGTTGTTGTTCCTGAAGACTTCTTTGGAGATGTTATGGGTGATATTTCTAGAAGAAGAGGACAAGTTAGAGATAACGAAACAAGAAACGATGGAGCCCATGTTATTAAAGCATATATTCCATTGAGTGAAATGTTTGGTTATGCAACTCAATTACGTTCAATGACTACAGGTCGTGGAACATACCAAATGTGATTCGATCACTATGAAAAATTACCACGTAATTTATCAGAAGAAATTATTAAAAAACGTGGTGGAAAATTAGTAGCTGACGAAGATTAATAATATTAAAAAAGATACTGCATTTTGTAGTATCTTTTTTAATAACTAACTTTAAAAAATTTATATAATTGTTTTATGCCTTCAATTAAAAAAAATACTAATTTAAAAGATTATCAATTAAATAATAATAAAAAATTGCTAAAAGCAAGACATCGCCTAACGTTTTGATTTGGTGTAGTGATTTTATGTTTATTTGTTATAACTTTTGTATGTTCATGAATTAGCGATGGACATGAATTAAAAGTTGCTTTTTCTAAATTGAGTCAAGAAACTCGAGATAAATTAATCTTAGCCAACGCCTTGCCTTCTGTTTCGGGTGCAATGTTACAAGTTAGATTTACATTTACCTATATTAGTAACATTTTAATAGGTTTTGCATTGATATTTTATGGTTTACTTCCTAATTATCTATGGACAAAAAGAGGATTTTTTTTAGCAACTGTTTATATAAATATAACATTCTTTACTTTTTGAGGTTTAATAATACCTTTTGTATTCATTAATCCAAATATATGATATGAAATTCCTTGGAATTGAAAAGTTTTAACTGTTCCAGTTCATTTAATAAATCCCGTTATAGGAACAACATTTTTTATAATAAATAGACGAGAATTGGTAGTCTCCCATAAGACAATGCTTTTGAGTTCAATTTTTATGCTTGGATATTATTTATTTGCTGTCTTAATTTTTTGAATTGGTGTGCAAGTAACTAATTTATTTTTAATTGAACAATTTAATAAAAAAACAACTGGGCTTGATATTTTTACAAAAACTAAGGTGGCAATCTATCCCTTTTTAAATTTAAAATATCCTCTTGGTTATCATGGCAATAATTTGTTTTATAAAATTCTATTAAATTTATTTATCCCAATATTTGGAATTATTGTATGTATATCTGTTGCTTATTTTTTAAAATTTATTTGTAAGATTCAAACGTATTCAAAAAAAGATTTTGAAAATCCTGCGTTTTTAAGAGAAAATAAGCTCTTAATAAAATCAATAAAATCACAAATTTTTAAGTAATAAGGAAAATCATGACGGCTTTTAATTTAAAAAAATATTTTGAGTTACAAACAATTGAAATTAAAAATAGAATTAAGCAATTTAACAATAAACTATATTTAGAATTTGGTGGCAAAATTTTTGACGATTTACATGCTTCAAGGGTTTTACCCGGCTTTTTAAGCGATAGCAAATTACAATTATTGTTAAAATTAAAAAACGAAGCAGAAATTGTAGTTGTAATTTCTGCTAATGATATTGAAAACAATAAATTGCGTGGTGATCTAGGAATCAAATATGAAGACGATGTATTGAGACTAATTGATAACTTTAAAAGTTACGATTTAATTGTAAATAATGTAGTTATTACCCAATATCAACCAACATTTAGAGTTAATAAACTGCAAAACATTCTTAGTGAACATAATATCAATGTCGTTAAACACTATAAGATTGACGATTATCCTAATAATTTAGATTTAATAGTTTCAAAAAATGGATATGGCAAAAATGAAACTATAAAAACTCAAAAGCCATTAATAGTAATTACTGCCCCTGGACCAGGTTCTGGAAAATTAGCAGTTGCATTAAGCGAACTTTATCATGATGCAATAAATAATATAGATTCTGGTTATGCAAAATTTGAAACTTTTCCCGTTTGAAATTTAGCAGTTGATCATCCATTGAATTTAGCTTATGAAGCTGCAACAACAGATTTAAATGATCAAAACAAAATCGATCCTTATTATTTAAAAAGTCGCAATAAAATAGCAACAAATTATAACCGTGATATTCAAGCATTTCCAATTTTAAGTCAAATAATGAACAAAATTCAAAAACGTAATATTTATGATTCACCAACATCAATGGGAGTTAATATGGTTGGATTTTGTTTTAATAATGAAGAGGAAATTAAACAAGCAGCAAATAAAGAAATAATCCGTCGCTACTATCAAAGTGCTGTTGCTTTTAAAAAAGGTATGGTTTCTAAATCAGTATTAATAAGAAATGCAAACTTGATATCTAAAAATAATATTGATTTTTCAATTAGACCTTGCATTGAAATTGCTAACAAATTAGCAAATCAATTGAATATGAATATCACAACCTTTACCTTAAACAACAATATAAATATTATAGGTAAGACATCAGCGCTTCTTGGCTCATCATCAGCTGCGTTATTAAATGCATTAAAATATTTAGCAAATATTCCTAAAGAAATCCATTTACTATCAAAAGAAGCAATTGAATTAGTTCAAAATCTTAAAGTAAATGTTTTTAAAAGCAAAAATCCCCGCTTACATATGAATGAAACTTTAATAATTTTAGCAATGTCTGCTAAGAGCAACCAATATGCAAAATTAGCCTATGATCAATTAAAAAATTTAGCAGGTATCGAAGCACATTGCAGTGCAATACTTTCTATGCAAGATATTGAAACATACAATCATTTAAATATTAACATTACCCAAGAACCAAAATACGATAATGACACCCTATATCATAAATAGAATATTAAAAAAACATCAAGTTGTATTGTTAACCGCTTGATGTTTTTTAAATATTATTTTATATGAAAATCTAAATCAAAATCGTTTTCTATTGCTTTGCATTCAATATTGAAGGCAGCAAAAATTTTATTTGAAGCTTTAAAATCATCTGAATCAGGCTTATATGCAACTGCATAAACAACTTTAGAAATCTTAGATTGGACAATTAGCTTAGCACAGTGGTAGCATGGTGAATGTGTTACGTATAATATCGACCCTGGTATAATCTTTGAATTTGTAATATTTGCATTTATTATTGCATTTGCTTCGGCATGGACAACGTAAGTATATTTGGAATTTAAGACATCGTTTTGGGTCGCAGGTCTATTCCAAGTGAATAAATCATCATTGTTAACTTCTTTATTTAAATAACTTGTTGGCATACCATTATATCCTAGCGATATTACATAATTATCTGGACTTACAATACAAGCTCCCACCTTAGTTAAAGGATCTTTTGAACGTAATGCACTTAATTTGGCAAGTGACATAAAATAAATATCCCATTTTAAATTATCTTTATGGTTGTTAAAAATTAATTTTCTTGTTTTATTATTCATAATAAAATATTTTAACATAATAAAGACAAAAACAGGCCCCTCAAGTCCTGTTTTTAATTATTCAGTGAAATAAGCTATAAGCTCTGTTAAGACATTAACTATGTTAATCCAATTTACCTGAAATTGAATTGCTATAATTATAATAAAAAATTAAAAAAATTTTTATTTTTTGCAATATTTTGTTTTTAATTGCTAATTAATGCAAAAAATACACCTTAATGTTAAAAAAAGTATACAAATTTAAACATAAAAGCTTAAATTGTTGTAAAATAAGTCTATAAAAAAAAGAAGGAAAAAGAAAACATGAATCAGAGTTTGAAAAACCAAAGAAACTTAAAGAATATGTTTAAACCAGCTATGTCTGGCTTTTTCGCTATATCAATTATTAGCTTTATTATGAAGAATGTTATGAATTGCGTTATGTCTAGAAACATCAACTATATTATGACAAACATCATTTGAGGAGCGAATAATGACTATCAATTTTTCAACTATAAATAATTTTTTTAACAATCAGTATATTAAGGCAAAAAATAATCTTAAAAACTGAATAATGGCTTTTAGAGGTTCATAGCTTCGTTTAATATTTTTATATTTTGTATTTAAGAATATAAAAAATATTAAAAACATAAATTTATTAAACTAGCTAATATTAATATCAAAAGCCAAATATTTCAAATATTAAGTTTATTATTCAATTATTTAATAAAATTACTAACATTTTTAATTACTTAATGACCTTTAAAATTAAAAATGTTGGTATAAATTACAATAACATTCTTTAAACAATTATTGTTTAAATATTTACAAACAAGATATTTGTATATCAACAAAGATATGCAAAACAAATATATAAAAATTTAAAATTCTAAGGATAAAAATGAAAACTAAATCAAAATTATGATTACTTGGTCTTGGGTCCTTACTAGCAGTAGGAGTACCAGGATTAGTAGCCGCTTCATGTAAAATAGACCCGGCATATGTAAAAGGCGAATTCGTATATGAATTCAACGCAGTAAACAAAGAACCTATATTTGATGCAGACGTTTCAAGTTCATATGGTCAATATATAAACTCATTGCCTGCAACATATACAGGTAGTGAACTTGTTAGAAAACAAGCCTTAACTCAAACAAAAACTATAACTATAACAGAAAAAGAAACTAAAAAAACCACTACCTATTTAGTAAAACCTACTTTTGCTAAATATAGATTAGAATTAGCAGAAGCTATTGTTTTAACTGAAAAAGAAACTAATAAACAATATGTATTTGATTCAGATGAAATTCCAAGTGAATCTGAATATCCACAACCAACTAAAACTGTAACAGATATTAATGGCAAAGAATACAAAGTTTATGAAAAAACAAACGTTTTTGTTAAAACAAAAGGTGACAAGAACGTTGAATCAAGATCAATTAATAGTGCATTCTTTTTAGACAAATTAGCTAATGCCTCAAAATTACAATTTGTAATTAGAAAAGGTGTTAAATGAGTTGATCACGACGGAAAAGACACTGGATATGAAGTTAAAGCAAAAGACTTCTGATATTCATGACTAAGAACATATTCAAGAAACTTAGAATATCGTTTAAACACCGGTGGATCAAAAGAACTAGATGAAGCATTGAAATCAAAATTAGCTGAAGAAAATTCACCAGTTTATGGTGAACAATGATCATTTGGTAACATATATACTTATGCATTGTTCGGCATTGATGCTAATAAATTATTTGAAAAAGATAAATTTATTCAAAAAGTATCAGCTGAAGGTAATTTAAAAGATAGAGAAGCAATTACTTTTGAATCAATTAATGGAAAAGAAAAAGATGCTAACATTTCAAGCTTCTTTGAATCTAAATTTGCATCAGGAACATATGACTTTATGCCAGCTCCATCTGACTACATTGATGCATTAAATGCTAAAAATGAACAAGTTGTTTTAAAATCAAATGGTATTGCTTTATCAAAATCTGAAACAGAAGCATATCGTAACGCTTTAAATTCAATGGATAAAGATAGCTTACAATACAAAATTGGTCAATACTGATATGGAACATCTGCTAAATCTACATTGTACTCAGGTTACTACTACAACAAAGGTATAGAAAACCAAATCCAAAAATTACCAAAGAATACACTATACTGAGATAAGAAATGAGTTGAAGATGATTCAACAATCAAAACAATCGTTTTCAACTTCCAATCAAAAGCAGTTGAACCTGCATTATACAACCAAACAGCATTTAGAAAATATAGCCAAGGAACAATTAGCCAAATTGGTTTTAGTAGTTTAACAGAACCACAAAAGAAAGAAGTTTCTTCAGAACCAGGAAAATATGGATTGAAATTAAATGAACGTTTGAATGTTCAAACTTCAATCTTTGGATTTGTTAGAACACCATTTATTTCTTCAAAATTAGGTAAAAATTACAAGTTCAATGAAAACTTCGCTAAATTAGTATATGGATCTTCACTAGCTGATATTGACAGTGGAAAAGCAGACACTTCAGAATCATATCTATTTGGTAAAGGTTTAAGCTTCAGAACACTTCTATATGCAGCAGTAAACTGAGATACATATGCAGCCCAAGCATCTTCTGGCCAAGCAACTCACTGACTTGCAAAAGTCCCAGAAGGGTCAAATTTAGGTGGTTCAGATCAAGGCACTGCAAAATTGAATACCATTACAGACTTCAGAGACCAAGCTAACTCACTATTTGCAATTGACAAAGATGGAAACAAAGTTGATTTCGGTGATAAATTAGGTAAGGAATTAAGTCCTTCTGAAAACGCTAAAGCTTCAGAAACTGAATCATCAATTTCTGAAAAGATGAAATCAGCAGGATTTAAAGTTCTTAAAGAAAAAATGAAAGAATTACTAGATGAATTCTTTAAAGAACAAAAAATTACCGATCCCGAAAAACAAACAATTGAATTTGAAGTAGGATTTAGATATTTAAATGCTCCACAACTATACATGAATGCATGAGAAAAAGTTAAAAACGTTGTTTCTGAACTAGATCCAAGAATTAAAGTTACAGTTAAGAGCTTTGATGACCCACGTGATCCAAACTTCAACGCATATAGATTTGATGGTGTGACAGGTGAAAACTTATTAGCATGATCAGCAGACTATAACGGTGTTGCTTCAACATACGACGGACTTTCATGAGGAGCTGCATTAGTTCCAACATTGGTAAAGGTTGCACACCAAACAGGTGACCAAAAAACTAAATTTGAAAAAGCCTTCCCAGAAATTGCTAAGTTGGCTAAAACATTGTTAGAATATGCAAAAACAAATAAACCAAATCTATCATTGGATTTTGACGAAATTGATAAAATTGACAATAGATATTTAAATTCAAACTTACCTGCAATTTTAGCATCATATAAATTAGAAAAGGATCCTAAAACCGGAAAATATGTAATTGCTAAAAAAGATGTTAAAGGTTCAAAACCAAAACCAATTGAATTCGATTTTGGAAAAGACAGTTCAGGAAAATCAATAGAACCAACCGATCTATATCAATGATCTGCAATATTCTGAGCAAGCACATTTAAGAACTTTACAAATGAAGAAATTTCTAAGATTGTAGAAGAATTTACAACATTTGTAACCTTAGGATATACTAGTGACGTGTTTGTTGGCCGTGATAAATTTGTTAAAACTTTAATAAACAAACACTACGTTTCGCCAACTATTGCAGGATCAGCAGTTGATTCATACCAAGACTGAAGAATAATCAAAGATACTAAAAAATAATTAATTCAATAAAAAATGACAAAATATATATTACAAAGAATAGCGTTTGCTATTCTTACTCTATTTATTATCTCTATTTTCGTTTATGTGCTGGTTGCTGCATTTGGAAACAACCCAGTTAATGACTTAGCACAAGCACAATTTCAAGCAAGCAAAAAATCAGGATTAACATATACTGAAATATTGCATAAACTTGAAATAGAAAACGGATTAAGATATAAAGATAACCCGGACGTTTTAGTTCCTATAATTGTTAGATATGGCAATTATATGAAAAACATTTTTACGAAGGGCGATTTTGGATTTTTATATAATCCAGCTAATAACCCAAACTCTGCTTTATATACAAATATGGCAAAACTATTCTTTACCCCCCTACAATATTCATTAATAATCACTGTTCCAACATTCTTTATTAGTTCAATATTGGGTATTGCAATAGGTGTCATTGCCGGATATAAGCGTGGAAAATGATTTGATGTGTCTTCAAATGTTTTTGTGTTAATATTTGTTGCTATACCATCATTTATATTGGCCCCTATTGTAATAACTATTTCGGTCGCATTAGGAATTTCTAGCGTTGTGCCTAGACCTGGTGAACAATCATTTGGAGTAATGTTTGTTTCTTATTTACCTCCTATATTTGTTATGTCAATAACCTCATTAGCAGGTTATGTAACATATACAAGAAATCAAGTTATTACAGTATTGACATCAAATTTCGTTTTAATAGCTAAAACAAAAGGATTGAGTTCTTCACAAATTTTCTTTAAATATGTTTTAAGAAATATTTCAATTCCTTTATTTAGCCTAGTTTTTGGGTCATTTATTGGTCTATTATCAGGATCAATAATTATTGAACAATATTGACAAGTACCAGGAACATCACAAATAATAGTTAATGCCTTCCCTACAGGAGAAATTAATGTTGTTATGTTCTCTACATTATTCTTTACATTTATATCGCTAATTGCCGATATTATTATTGACGTTTCATATGCAATATTAGATCCTAAGATTAAATATTCTACTAATAGCAAGAGAAACTATTGATTATTTTTCTTGGCATATAGAGAACGTTCTAAACTTGCAAAAGATTTGTTTAAATCACAATTAATAAACAAAGCAAGTCAAAATACAACTGTTAAAGGAGATAACTAATATGAATATAAATGAAGTTAAAGCCTTTAACAAAGAATATAAAATTAATACTGAATTAGAGAAAAAATTTAAATTTGTTGCACCAGAAGATAGAATAGTAATCTCTTCTGTTGCAGGTAAGCCTAAGAAATTAGGTATAGAAATTTTAAAGAGATTTTTTACTAAACCAGTAGTATTAATAGCACTAGCAGTATTTATAATTATTTTACTGTTATCTATATTAGTTCCTGCTAATGGAATTTCTTACTATAAACCAAACGATCCAATTTCAGATAATGCATTTGTAAAGAATTTACCACCTGCATCTTCTCCAATTGTTACTCAAGCATTAGAATCAAATGATCCCGTATTCAAATTTTATCAATTTGTCTTAAATAGGTCAGAAACCGATCCTGTATATAAGGCTAGAATGGCATTTTTCTTAAATGGCTTTCAAGCAAAAGTTAATGATGTTGGGATCTATGTTGCAAAATATAATGCTTATGATTTAGTAGTTGCTAATTACATAAATGGAATTATTTCTAAGAATCAAATAACAGATGCTGAATATTTAAAAATATTTAATGAACAAAAAAACTTCTATTCTCATGTTTTATTAGGAACATCATCAATAGGCTACGATATTTGAACTACCGCATGATATGCAACATGAAGAGCAATTAAAATTGCTTTAATAGTTGTAATATTGGAAGCGATTATTGGGATATCAATAGGAGCATTCTTAGGATTTTATGCTGGAAAATTAATTGATACCATTATAATGCGTGTTATTGAAATATTCCTAGCACCTCCTACATTAATATGAATCTTATTATTTGTTTCAATTATTGGAGCAAATGAATGGTCATTAGTTTTGACGTTAACAATAGTTGGATGACCAGGATTTGTTGGAATAACTAGAATGTTCATTATTACAGTGAAAGATGAAGAATTCATCATTGCTGCAAAAGCAATTGGAGTTGGTACACCAAGAAGAATTTTCGTTCATGCATTACCAACTATAATTGGAAAAATTGCAAATAATATAGTAAGATCAGTACCAGGTGTTATTCTATGAATAGCATCACTAGCATTCCTTGGATTCTTTAAAGAAAAGAAGGATACTAACCTAGGACAAATGTTGATTGAAGCTTCGCAAGAAGTTGGCTCAAATATTTGGATTATTGCTTTGCCTACAATTATTTTATTGTTTTTATCATTATCATTGAATTTCATTGCCTTAGGTGTGCATGATGCACTTGACCCACGTGTAATGAACAAAGGAAAAGGTAAGTAAAATGGCAAAAATTATTAATAAACACGACAATTTAGATAAAAATAGCTCAGATACTATAAAACATCAAAAAATTAATAAAAGTACTAGAGTTTTAGATTCAATTCGTGTTGAACCTCTAAAAAATAATCAAATATTAAAAGTTAGAAATCTGCATGTTAGTTTCCCTCAAGGTCATAAGAAAAGTATCCATATTGTTAGAGGTGTTGATTTAGATGTCCATAGAGGTGAAATAATTGGCCTCGTTGGAGAATCTGGTTCTGGTAAGTCAGTAACTTCAAAAACTTTAATTAATGTTAATGAATCAGGTATTATAAGTGCTGATCAAATTCAAATTGGCGAATATGAACTAACAAATATTAAAAAACAAAAATATTGACAATATATAAGGGGACAAAAAATTGGTTATATTCCACAAGATCCCCTAACTAGTTTAAATCCAACAAGAACAGTTGGAAAGCAATTATTAGATGCATTAAATAATAATGAAGATTGAAAAAACTCAACTTATACTGAAAAGAAAAATTATTTAATTGGTTTATTAAAAACATTTGGATTGCGTTCGGCTGAAAAAATATTTTATGCATACCCTCACACTCTATCAGGGGGTATGAAACAAAGAATTGTTATTACAATGGTTGTTGCTTTGAAGCCAGAATTAATAATCGCTGATGAACCAACAACAGCATTAGACCCAACAGTTCAAGCCAGCGTTTTGGCATTATTTGAAGATATTAGACAAAAAATGGGAATTTCAATAATTTTAATAAGTCACAATATATCTGTTATTGCAAAATTTTGTGATTACATTTATGTTATGTATGCTGGTAGAATTGTTGAAAAAGGAACCAAACAAGAAATCTTTACAAATCCTGCCCACCCATATACTTGAGCATTAATTTCGGCCATACCCGAATCAAAAGATGAAAAACTATACACCATTCAAGGAACCCCTCCTGATATGGCAAATTTACCTCTTGGTGATCCATTTGTATACCGTAATGACTACGCATTAGAAATAGACTATTTAAAAGAACCTCCTCTAATTCCAATTACTGAAACCCATGCTGCCGCAACATGATTATTATCTCCTGAAGCACCAAAAATTGAAAAATCAAAAGAATTACAAAAACGTTTAGACTTATTTAAGAAGGCATTTAACAATGAATAATAAAAATTTTAAAGATAAAAAAGTTATCTTATCGATAGATAATCTAAAAAAATACTTCGTTAATCAAGGAATGATAAATAAGGCTGTTGATGGAGTTTCTTTTGATGTACATGAAGGAGAAATAGTTGGTTTAATTGGAGAATCAGGTTCAGGTAAAACAACTGTTGGTTCAACAATACTTAGACTATATGATGATTACAACGGATTTGTAAGATTAGAAGACAAAATAATTTCAGGTAAGCGCATTAGTGAATCTTTAAATCGTTTTTTAAGAAAAAATGTGCAAATGATTTTCCAAGATCCACATGCTTCATTAAATAGTCAACAAAATATTTATTCAATTTTAAAAGAACCTTTATTAGTTAATGGAGTAATGAAGGACAAAATTAAGGATATTTTTAAAGATTGATTAAATGTTAAAAAGAATTTTAAATACACATTTCAAATCGAAGCAATGAGATTGGAATTACAAAATTATTTAGAAATTAACAAATTAGCTGAACCTTTATTTAAAAAGTGAACAGAAAACCTAAATAAGCTAGAATTTAATTTTGACATTTCTAGAGAAGATAACTTTAGCTTATTCTTTGGATATTTAGAAGAAAAGCAAAACGTTGAAAGCATTATTATTAATAATATGTATGCAAATGTTGATAGGTTAATTGAATTTTATTATGAAACACAACGTAAATATCGTAATAAAGAATTACCTGGACCACAAATTGCTTTAAATGAAAAAGAAGAAAAATTAAAATACATTCAATGTTTAAGCAAGACTTCAAAGATTGCTTATGAAGCTTCACTTGAATTAAAAGAAGTTAAAAAACAAATATTTAATTTAAGACATAAAATTGAAAAAATATCTTCTGAAAGCAAGAATACATTTGCAAACTATATTCATGAATCAAAAAATGAAAAGAGTTTAATTGACATAGCAAGATTAATGTCATGTGATTTAGACTTTTATTCATATAATTTAAAAAGTGAATTGTTGATGAATAAAAGGGGCCAAGTATTGGCAAAAATAAAACCATTTAATAAATTCCTAGGATTTAATAATATAAAGAAGTTAATTAATGAACTAGATGAATATATTAATGAATTTTATAAAACAAAATTAGCCCCATTGCCATATTCAAAAACATTGAAATCTGATATTAAAAACGTTTTAGAAAAAGACTTTGATTTTAAATTCGATGAATATACTAAAATATCAGAATTAGAAAAACAAAAGTTAGATTCAGAATTAAAACAATTACTATCAAGAGAAGTTGAAATTAAAAAAGTAATAAAAGAAAATCCAAATCCTGAAATTACTTCAGAACAACTTCAAGAAGCAAAAAACGATCTAGAATTAGGAAAACAAGCATATCTTGAAGGCAGATCTAAATATTTAATTTCATATAAAGCCGAACTTGAAAGCTTGTATAAAAATCTTGAAGAACAAAAGAAATTCTATCTTGAATTGCGCAAGCAACAAGATTATTGCAATAACAAATATAAAGAATTGAAACAAAAATTCTTTGAATATGTTGAACAATTACGTCAAAAAGAAAAAGATAAGATTTTATTAAAAATAGCTGAATTAAAACAAAATCAAGACAAATCAAACAAAGATAAAAAGTCATTAGCAAAATTACAAAGCAAATTAAAATCAATTAAGGTTCAATACAATACAATATTAAAAATGTACCATTCAGATATTTCATTAAAAGAAGATACATTAAAATCATTTGATATTGAAAGAAAATATCTTGATAAAGATATAAATAATATTTATGTTTTATTAGGAATTGACCACAAGTGAGTAGAAACTAATTTAAAAACAGCTGATGCTATGGCTGGAGTTGATGCTAAACATATGAAGTGGGTATGACGTAAAAGATATTTTGATAGTAAAGTTTCATATCCTATTGCAAAATTATTAATTTCTTCAATATTGTATAAAACAATAATTTATAAAGCATTGGAAGATGTTGGACTATTAAAACAATTTGCTTATCGTTATCCTCATGAATTTAGTGGGGGTCAATTGCAAAGAATTGTTATTGCAAGAGCATTGATAGTTGAACCTCAAGTTATTGTGGCCGATGAACCTATTGCCTCACTAGATATATCAATTCAAGCGCAAGTTGTTAACTTGCTAAAAGAATTATGTATTAAAAAGAACATCGGTCTTATATTCATAGCACACGACCTTTCAATGATTGAATATGTTGCTGATGAAGTTCAAATAATGCACCTTGGAAAAATTGTAGAAAGTGGAAAAACCGAAGCAATATATGCAAATCCAATTCACCCATATACAATAAACTTATTTAAAGCTATTCCTAAAATTTCTAATGCTAATGAAAAATTCCAAAATGTTTCATTTGCTTTAGATTACTTAGATGAACAAAAATTCCCTAATGTTCCTGAAACATTTAAAGTTGGTGAAGATCACTATGTTTATGGAACTGCTAGTCAACTTAAACAATGAACACATAATGCAAAATTAGAGAAAGAACATAAAGAATTATAAAAATAATTTACCATTAAACTAAATAAAAAAATTAGACTCTTGACTAAAAACTTAAGAGTCTAATTTTTTTATTTCTTTATATAAGCTAATAATTTATTAATTTCGCTTGAAGTTAAATTTCTATATTGACCTATTTTTAAATTACTATCATCAATGCATCCAAAAGATTTTCTATGTAATTTTTTAACATAATTATTTACTAACAAAAATAGATTTTTAACGTGATGGTTTCTGCCTTCATATAGTAGAACTTCGTATTTAAAAGCACTTAATTTTGTAACTTTTTGTTTTGAAATTTTGCCATTTAATAATACGTGATTTGAATTTAATAAGTTTAATTCACCATCAAGCATTTCACGTTCTAATGTAGCAATATAAATTCTAGGAATTTCAGAAGAAGGATGGCTTAATAAATTAGCAAATTCTCCATCGTTTGTGATTAATATAATTCCTGTTGTATTAAAGTCTAATCTTCCTATTGAAAATAAGCGTTCTTTTGCTTTAAGATATTCGTAAATTGTTTTTCTATTTTGAGGATCACTTGCTGTACAAATTGTATTCATGGGTTTATTTAAAACAATGTATTTAAAATTATTTGCAACATTATTATTTAATTTGATAATTCTATTGTTTATTTTAATTTCGTCATTTATAGTTACACATTCGCCTAATTTTGCAATTTTATTATTTATTAAAATCTTCCCCGATAAAATTAATTCTTCTGCTTTTCTTCTAGAACAAATGCCTTGCTCACTTAAAATTTTTTGAATTCGTTTTGCTTTTTCTTCCATTTTTAAAACCTTACTAAAAAGATATTTATATATAAATATATCTATTGTCGAGTGCTAAAATTATAACTTTTTTTTGAATTTATTTTTTAATTGGAGTTGGAGGAAAAATGGAATATTTACAAAATATAGACTTAAGCAAAATAAGATCGCTTTGTTTACAAAAAAAGCGGGAATTTATCTTTAATTATTGCCTTTTTGAGCAGCTAAAAGATAAACAATTAAAACAAAAAAAATCACAAACAATCAATGACGAAGAAGATGATGATATTTCAAAATTAGAAAAATTTATTAAATTATTAAATTCTGATAATCAAAAAATCTTTTTTAATAATTTTATTTATAACAATCCTGAATCTGAATGATATTTGGCATATTGATCAAAGAATGCATATTACAAAAAACTTAATTATATAGTTAATTTATTTATTGATTTTATTAACCAATAATGTTTCCTATTGTTATGCAGGCAATCAATGATCAAGAACCAACAAAATGGATTGAAAACATAAATGCTTATACAACAAAACAATTATACTTAGATAAATGAACAAATTCATTTCAAAAGGTTGATTTTAAATTACAAAGGTTAATTGAAAATAATAAATTTATAATTGAATTTAAATATTTGAATTACGACCAAGAAATAGATGATATAGATGTTAAATGTTCGATTAACAATCAATTATTGAAACTCCAAAAAGTCTTTGATTCAGATAAACATGAATATACATATACTCTTATTTCTGAATTTAATGAGGCAACAAAAACTAAAAAAGTTCAGGACATAAAAAATATTAATTTTGAAATTTTTTATAAGGTAAAAGGTTTATTGTATCAAGTAGAAAGATATACATATGAGATATATAGCAATAACAATTTAAACAGAGAACTAGTTTCAAATAAAAATGGTGTCGAATATGATTTATGGACTAAACTAAGAATTATGTCATTAGCAGATAGAGACATTCCAACAGATATGGAACATTATTCATTTGTTGAACAAGTTAAATACAAATTTATACCAACTACTCTGGTGTTGGGAAAACATAATAAAAAATTATTTGATGTTGAGATATCAAAGAAAGAAATTTCTAACAAAAGTGGCATAATAAGCGATTTTTCTCAACTAATTCAAAGCGATATTAATGGTTTTAATGTATCTATTGATATTGATAAACAATTTATAAAAGGCAACTTGAATGCAAAAATTAATGATTTCAGTAAAAATGAGCATAAGCAAATTTCAATAGATTCTTATTCATATTATGACAAGGTTTCTAAGGCAATAATAGCAAATAATGATAGTATTGGCGCAGCTAAAGGCTTATTGTTGCCTCTTAATTATTCAGGAAATTATTCTTATTTACTAAATATTAATTTTGGTGAGGATTTGAAAGGTTTTAAAATTAGATACAATCAAGAAATTTCAAAACCATTTTTTAATGAAAAAAGTGGCTTAGTAAAATTAAATGTAAATAATATATTTAAACAACAAAATTTCATAAAATGAAGTGTCGTAAAAGCAGAGGATATAAGAAGTTTGATTAATAATTCCAATACTCTAGAAGAATTAATGAAGGGAGGGAAATAATGTTGATTGCAAAAAAGAAAAAAATAATTTTCGGTTTATCTCTTGGATTAGCTTGTGCCGTTGGCGCTGGCGCATTAGTTGGAGTTTATTTTTTAGCATCTTCTAATTCAAATAAAAAAGCTAATAATCTAGACAATAAAAAGGTTAACAAAAAAGATTCAACAAATAACAATAGTGACAACAAAAACATTAATTCTGGCAATGAAAATGAAAAAGATAACACCATTGATAACCAAAACGAAGATAGTAAAATTGAAGATTCTAATGAAGATGGTCAAGCGAATGATGACGATAAAAAGCTTAAACCAGAAACAAGGCCAATTATAAAAACAGAGATTAATTGAAGAGAAATATTTCCTGATGTTAAAACTTCAGATTATTACAATCAATTAAATTTTAAAGATGGCCAAGGATTTATTGACAAAGAAATGATTACTTATATAATAAAAGATATTTTAAATAGAATGACTATTTTAGATGGCGATGTTTCTTATGCTTATAAACAAGAGGATGATCAAAATTTATATATTGCTTTTAAATGAGAAAATAAAAGTCAAAAATCTAATATAACTTATAAATTTAGTGTTAATAGAATATAATTAATTTTATGGAATCTGAAAAAATTATAATTAATGCTATTAAAAAACTAACTAAAACAAAATTTAATAAAGAAACTTTTATTCGTGATTTAAATATTGATAGTTTAGATTTGGTTATACTTGTTAGTGATTTAGAAGAGGCTTACAATATAAAAATAAGTGATGAAGAATTGCTTTCATTAAAGACCATAAATGATATTATTGAATTATTTGATAAAAAAACGAATAAATAAAGCAAAAATAATGTAAAATTATATTGCTTTATATGGGTATAGTTCAATCGGCAGAACAACGGGCTTCAACCCCGTGTGTTGTGAGTTCAAGTCTTACTACCCATGCCATATCAGAAATGATTAATAAAAGTAGCAAAATTTTGCTACTTTTTTTAATTTAAATTATAAAAAAAGTAGGGATTTTAAATCCCTACATGACATAAAAAAATGATATGGTGCCGTCAATTGGAATTGAACCAACGACCCCTTCCTTACCATGGAAGTGCTCTGCCCCTGAGCTATGACGGCATTAAAAGCAAAATTATTATAACAAAAAAACTAAGTTTTTGCTTATAATAATAAAAATAAGTTATTAATGTTATTAATAAATTAATTTAAATTTAATTATAGAGGCTATATGGAAAAGACAATGTACGATTCACTACTTTCAATTAAAAATCAATATGATGAATTAACTGAAAAATTAAACGACCCTAATATTTATAATGATATAAAAGCATATACAAGAATAGCAAAAGAAAAATCTGAGTTGCAAGAAATTTATGATGCTTTCACAAGATATCAAAACATTGAAGAATTATATTTCTTGTCAAAAGAAATTTTAACAAACGAAAAAGATCAAGAATTAATTGAACTTGCAAAAAACGACATTGACAATCAAATGAATCAATTGCAAGAAATAGAAGAAGAAATTAAAGAACTTTTATTGCCACAAGATGAAAATGACAAACGTGATGTTATTATGGAAATAAGAGGTGCAGCTGGTGGTGATGAAGCAAATATCTTTTCTGGTGATTTATTTAAAATGTATCAAAAATATGCAGAAATCAAGGGCTTTAAAATCAAGATAATTGATTCTACATATGGTGCTGCCGGAGGATATAGCCAAATTGTTTTTAGTATAAAAGGCGAAAAAGTTTTTTCACAACTAAAATTTGAACGTGGTGTACATAGAGTTCAACGTGTTCCTGCAACTGAAACTCAAGGGCGTGTTCATACATCAACTGCAACTGTAACTGTATTGCCCGAAGTTGATGAAGACGTAAAAATTGAAATAAGACCAGAAGATATAGAAGTTAATGTATTTAGAAGTTCTGGAGCTGGCGGACAATCTGTTAATACAACGGACTCTGCAGTAAGAATTACTCATAAGCCTACAGGATTGGTTGTAACATCACAAGATGAAAGAAGCCAAATTCAAAATAAAGAAACAGCCTTAAAAGTTTTAAAAAGTAGATTGTATGAACTAGAATTAAGAAAACGTGAAGAAGCAGAAAGCGGACTTAGAAAACTAGCTGGAACAGGGGATAGATCAGAAAAAATTAGAACATATAACTATCCTCAAGATAGAATTACAGATCATAGAATTGGCTTTTCAACTTCATTAAAAGTAGTTATGGAAGGCGAATTAGACAAAATTATTGAAGCATTAATAGCTGATGAAAAGGCTAAAAAAATTATTGAAGCGGGAAATTAATGATAGATAAAGAAATTCTACTACGCGAAAAAGAAAGATATAATTTACCCCTTTATATTTCAAAAAAAGAATTAAGAATGCTTAAAAAAGATGTTCCTGTGCAAAAAATAATTGGATACCAGGAATTTCAAAATGTAATTTTGAATTTAAAATATAAAACCTTGATTCCTAGATACGAAACAGAAGAGGTTATTTTAGCTGCATATCCATTTATAAAAAAAGAATCTAAAGTATTGGATTTAGGGTGCGGTAGTGGCCTAATAGGACTTGCAATTAAAAAAAATAAAGACGCTAATGTTACATTAAGTGATATTAGCCGTTTTGCAATCAAACAAACTAAAATAAATGCCAAATTAAATAATTTAAATGTAAATGTAATTCGTTCAAATTGATTTAATAAAATAACATCTAAATTTGATGTAATAGTTTGCAATCCTCCTTATTTAAAAAAACAAAAGAAATTAATAAAATCATTAAAATGAGAACCTAAGAGAGCCTTGTTTGCTAGAGACAACGGTTTATTTGCTTATAAAAAGATTTTAAAATCTGCATTTAACTATTTAAACGAAAAAGGAATAATTATTTTTGAAGTCGATCCTTATTGCTCTTTATGGTTTAAAAATAAATTTCCAAATGTACTAATACTAAAAGATATAAACAATAAAGATAGGATAGCATTGCTAGCAAAAAATGATTTAAAAAATTAGCACTCTTTTTAATTTTGTGCTAAAATTAAAAATTATGGAAAATAAACGTGATTATTATGAAGTTTTAGGCATTTCTAAAAATGCCACAGAAAAAGAAATCAAGTCGGCATATAGAAAATTAGCAATGCAATACCACCCAGATAGAAACAAAGCGCCAGATGCTGAAGAAAAATTTAAAGAAGTTTCAGAAGCATATGAAGTTTTATCTGACCCTGAAAAAAGACAAAAATATGATAAGTTCGGACATTCTGCATTCGACCAAAGTTCATTCGGATATTCAGAAGATGTATTTAATAGTTTCTTTAATTCTTTTAGAGATATGTTTTCTGGCGGAGGTTTTGAAGTTGATTCAAGTGATGATTCAGATGATCCTTTTTCAAGTATTTTTGGTTTTGGCTCAAATAGATCTAGAAAAACAAAGGGCAGTGATTTGCAAATGAAATTGTCAATTGATTTTGTAGATTCAATATTTGGAAAGAATGTAACTGTTCAATTAAAGAAATATAGCGAATGTTCACATTGTCATGGTTCAAGAGCAGAAAGTGATAGTGATATTGTTTCATGTCCTCAATGCCATGGTAGTGGTCAAGTTCAACAAAAAATGGGTTTCTTTTCAACAATTAGTCCTTGCAACAAGTGTGGAGGCTTGGGTAAAAGCATTAAAAACACATGCCATGTATGTAGTGGAAAAGGACATGAATATAAATTAGTGAATGAAAATATTCAAATTCCTGGCGGAATTAATAATGGCGAATATATTAAATTGTCAGGATATGGTAATCCTTCTCGTAATGGAGGATCTAATGGAGATTTATATATTATCATTGCTATTAGACCACATAAATTTTATGAAAGATTTAACAATGATATTGTAGTTTCAATGCCTATTTCTGTTTTTTCAATTATTAATGAAGATACCATTAATGTTCCCACACCATACGGAAATAAAGATATAAAATTAAATAAAAACATGAAATTAGATGGAGTAATCACTTTAGATGGCTATGGTTTTCCTATTAAAAATAGTTCTAAAAAAGGCAACTTCATTATTACATTAAAGCCTTATATTCCTTCGCTTAACAAAGAAGATAACAAAAAAGTTCAAGAAATATTTAGCAACTATAAAGATGTCGAATTTCAAAAATGAATAAAAGATTTTAATAATTAAATTTAAAAATGCATTTATTAATTTAAATAATTAATTGCATTTTTTTATATTCGAATAATTAAACTTGTATAATATTTAATTATTTTTTACTTTTTAATAAAAATAATAAATCAATGTATCCAAAAGAATATATTCAAAATAGCAAAATTATTTTTCAAAGGAGCAATATGGAAAATAAAATTATGATTGTTGAATCACCAAATAAAGTTAATACTATTCAAAAGATAGTAGGTGATAACATAAAGGTATTGTCAAGTATTGGCCATATTTTAAAAATGTCAACATCAGGCATTGGAGGATTAGGTATAGATTTTGAAAATTGAGAACCTAAGATGGTAATTGATCCAAGCAAGAGAAAAATTGTATCCGAATTAAAAGAAGCAGCTAAAAATGCTAGTGAAGTTTTAATTGCAACTGACCCCGATCGCGAAGGTGAAGCAATTGCAAATAATTTAGTAAATACTCTAAAAGTGCAAGATAAATATAAAAGGGTAAAATACAATGAAATCACTCCAGATGCCATTAATTACGCAATTGAAAACCCTTTAACAATTGATGAAAATTTAGTAAATGCTCAAAAAGCAAGAAGAATATTAGACAGGATTATTGGTTTTAAATTGTCTCAATTAATCCAAAGAAAAGTAAAAAATACTCCAACTAATCCATCTGCTGGTCGTGTTCAATCCATCGCTTTAAAACTAGTAGTTGATAAAGAAGAAGAAATTGCAAAATTTGTTCCAACATTATATTCAAAAATTGAAGCACATACTGCTGATGAACAAATTGCAAATTTCGTATATAGAGATAATTTAGATTTTGTTGGCGAAAATGAATGAATTAAAAGAGAAAAAGTAGATGATATATTAACTCAATTAAATCAAAATAAATCATTGCAGGTAATAGACTTTAAAAAATCTATTCGTTCAGAGGCAATGGTTGTTCCATTTAAACAATCTATTTTGTATAAAGAATCTCAATATAGTTCGGCAACTGTTCAAATCGCTGCACAAAAACTATTTGAAATGGGGTTAATTTCTTATCCTAGAACAGATTCAACTAGACTTTCATCAATATTTATTGAAAAATCCCAAAAATTTATTGCTCAAAAATATGGAAAAGAATATATAGCAACTGAAATAAAAGGATTTTCTGGTGATCAAGATGCCCATGAAGCAATAAGACCAACAGACATTTCACTGCTTGCATCCGAAGCTCAACAAAAATTTAATTTAAGTAATGAAGAAACAAATGTATATAAATTAATCTATGAAAAGACATTAATGGCTTTGATGGCTACACCAAAACGAGAAATATATCAATACGAATTAAAAAATGGCCAATATAATTTTAATATGTCATATAGTAAAATAATTTTCGATGGATATTATAAAATTTTTGATAAAAAACAAGAATCTATTATTCCTAATTATAAAGTTGGAGATATTATTAAAGTTTTGGAATTTGTTAAATTAGACAAAGAAACTAAAGCTCCTGCAAGATATAACGATGGTTCGTTAATTAAAATGCTTGATGATATAAAAGTAGGAAGACCATCAACATTTGCAACCACAGTTAAAATTATAAAAGATAGACTATTTGTTGAAACTAAGAATAATAGTTTATATCCGACAGATTATGGGAAACTAGTTTTAGAAAAATTAATTCAAGGTTTTCCAACTACAATAAATGAAGAATATACAGCTAGCGTTGAAAACCAACTTGATTTAATTTCAGAAGGATTATCAGATTATAAAATTTTATTGAAAGATTTTTGAAATAATTTTAATTTGCAACTTCAAAATGTAAATGAAACAATGGAAACAGATTTCTTGCCTCAAGAAATTTTGCAAGAAAAATGTCCTCTTTGTGGACATGACTTGCTTTATAGATACACTAAATCAAAACATCAAAAATTTATTGGATGTTCTAACTTTCCAAGTTGTAGATATATAAGAAATATCGACAATGAAAAACCTAAATTTTTTAAAAGAAGATATAATAAATAATTTTTATTTTTAAACATTACACAAAAAAACAAATGTATTAAAAAATTAGATTTGCAATAAAATCTAATTTTTTTATTATTTATTTTTATTGAGAAATTATTTTAATTTATTCCTGAAATTTTATCAATGATTATAGGATTATAATTTTTGCCCTTTTCATTGCTTCCTAATTTGTATTCTAGGCTAAGGGTCTTTTTATGATTTTCTCCTTTTGTTGAAATAACAAGATCATATTCATAGTTTCAGTTTATTGAAACAATACCTACAAATCTATATCTATTTTTTTCACCTTCCCATTTTGCTAATCCTTTGAAATTTTTTTGTCATCTACCCGGAACATTTTCGGTGCTGAATTCAAATGTAACATCTTTATAGCCCTTAAAAATTTTGTCTTTATCTCTTAAGTCTAAATCGTTAGGATTTGGTCTTCCAGTTTTTGAATAAATATCAATATTGTAAACATTCAATTTTCTATTAATGGTTCCGTAATATTCTTCAGTTTTATGACTAAATAAATTTTCACATTTTTTAATAAAACTAGTTTGAAGAGTTCTAACAAATGCATTTTCTAATATATCATCAAATGCACTTCACTTTACAGCAGGCCCTTCTAATTCATTTTGTTTTTTAACTTCTTCAGGCGCATTACCTTTGTTGTAGAATGTATTATAAAAATCTTCAAAATGAAGCATATTCATTTTTTCTAGTTGATCAAACACTTTTTGATCAACTAAATCATTGCATAAATTATCAAAAAATTCATTTTTTTCTGTTTGATTATTTTGTGTTGATGCATCTTCAAAAAATTTATATGATGCTTTCAAAATTCCTTCATATCTTGAAATTAAAGCCTTGTCTAAAAATTCTATTTTCTTAATGCATTCATCTTTTGCTTTATTGTCAGCATTTTTATTAATTAATTCTTTTTCCATTTCGGTTATGAAACCTTGGAAAATTGCCTTTATATTTTTATTTTTGTTTGAAAGACTACCTGAGGTGCCAATGTTTTCAGTATCTAAAAAGTTTAACTTTTCTTCAACTTTTTGTTTTTGGGAATTGAATTCTTTAATATTATTTATTATTCTGTCAAACTCGTTTTTAGCAGATTCTAATTTAATTTTAGCATTTTCTATATCTTGAATTTTTGAATTTTTATTTATGTTTGAAACACCTTTTATTTTATCTTCAATTTTTGTTCTAATTGAATTATATTTTTGATCCTTCAAATCTTTATTAATAAAACTTTGCAAATCATTTTTTATTGATTCAAATTTAGAAAATTCATTACGTTTCTTTTCTTGAATTTTAGTTTCTATTATTTTTTTAATATCATTTATTTTTTGGGTTGCTTGTTTAATTTCTTCATTTTTTGAAGCATCCGATATATTTTTATAATGGGTTAAAATTGTATCTGCTTCTGTAGTTCCTACTTTTTTAGCATCATCAGTTTTTATCAAACCTTCCAATTGATTTTTGCTGTTGTGGAAATCAGTAATATCTTTTTTTGTAATCTTTGCATTATTTAATTCATTTTGCAATGATTGATATGCATCTTGAATTTCTTTTTTATTATTAGAGAAAGTTATTGAATTTTTTTCAGCTTTTTTATTTTTTAAGTTTTCAACTATATTTAGATAATTGCCTTTAGTTTGATGATCATTTTCAATTTGTTTTATAAATTCATCAATATCTTTTCTTGTTTGTTCTAACTTTTTATATTCTTTAAATTTTTCAAATGCTTCTTTTGAATAAGAAATTTTTGATTCTAATTTATTATTTCCATCGCTAATTATTTTTGCGTCATCAGATTTAATAGTTTTAGCTTCTTCTATTGCTGAATTTAAATCATTCTTTATTGTCGAAAGTATATGATCATTATTATTTTTTAATTCTGATTCTAATTCTTTTTTAAAAGATTCAGCTTTTTCAATGGTTTTATGCAACTTTGCTAATTCGCGGTATTTTTCTTCTTCCTTGATTGCATTAGCTTTTGTGTCTTTTAATGTCTTTATTGATGTTTCAATTTCTTCGATTGTTGAATTTTCATCTATTTTTCTTACATTATTATTAAATAAATCTAAAACTTTTTGAATAGGCTTATAGAAGCTAATTTCATCAAAGTTATCTAATGAAGAAACATCAGTACTTACTTGATAATTTTCCAAATCATCTTTTGCGTTATTGAAATCTTTTAATAATTCATTTTTCTTATCTTTAATTTTTTGCTCTAAATTAGTTTTGGCATCATTTAATTCTTTTGTTGCATTTTTGATTGTTTCTTTATCTGTGGAATCATTTATTGTTTTCTTTTTGTTTAATAAATCTTGGGTTTCACTAGTTCCAACTTTTTTAGCATCTGGTGTTGATATTAATGTTTCCAAATCTTTTTTAGCAGCATAAAACTTATCAATTTCTGATTTTTGTTCTTTAGCTTCATTTAAAGAATTTTGAAGTTCGGTATTAGCATTTACTATGTCTTTTTTATTAGAATTTTCATTTATTGAGTTTTTTTCAGCTTTTTTATCTTTTAAGTTTTTAACTATATTTTGATAATTCTTTTTAGTTTGAGGATCATTTTCAATTTGTTTTATGAATTCATCAATGTCTTTTCTTGTTTTTTCTAACTTTTCATATTCTTTATTTTTTAATTCGATGAATTTATTAAGTTTTTCTTGAATTTCATTAGTTTTATTTGTTAATGAATTATTTGAATCAGTCAATGATTTTGTATTTTTTGAAGTAATTGCATTATTTGCTTTTTCTATTTCTTTTTCAAGTTGTTTTTTATAATTTTGAATCTCAGCATCTGAATCAGTTAATTTTTTTAATAATTCGTTTGCAGCAGATAAACTGGTGGTTAATTTATTATTAGCTTCGCCATTTGCTCTATCTGCTTGTTCTTTTGCTACTTTTGCTTCTTGTAATGCCTTAGTTAATTCATTATTTGCAGCAATAATATTTGCTTTATTTGAAGTTTTGTTAATTTTTTCTTGTGTTTTTAAAGAATTTTGTAATTTTTTAACAAGTTCAGCATAATTTGGATTGTTTTTAACATTATCTGTTAAGAAATCATTAATATCTTTCCTTGTCTTTTCTAATTCTTTAAACTTTGTTTCTTTTTGTTTATTAAACTCGTCAAGACTTGATTTAAATTCATCAATTTTGTTTGCTAATTGTTCTTTTGCTTCAATAATTGCTTTAGTATCTTTTTTAAGTGCTTCTTTAGCATTAGCAATTTCTTTTTCCAAATTAGTTTTTAGCGTAGTTATTGTAGAATCGCTATCAATTAATTCTTCTTTAAGTTTATCTGCTTCTTGAATTTTTGCATTTAGTTGTTCTTTGGCATCAATTTTTGCTTTTTCTTGTTCAGCGTTAGTTTTAGCCTTGATTAATGCATTTGTTGCATCTTTAATTTCTTGAATTTCTTTAGTTGATAAAGATTCTTCTTTTATTGCACTAAAACTATCTATCTTATTTTGTGCTTCTTTTTTAATTGAATCGTATTTTTGATTATTTAATTCATCATTAATGTATTTTTCTAATTCTTTTTTTGCTTTATCAAAGTTCAAATATTCTTGTTTTTTAGCATCTGAGATTTCTTTATTTATATTAGCTATTAATTCTTTAATTTCTTGTGATTTTTCTTGAATTTTATTCTTACTTAATGTTTCTAAATTCAAGTCTTCATATTCTTGTTTTTTATTATTGAATTCAGTTTTTTTAGATTCTTCTAATTGTTTAAAATTATTATTAGAATTTATCAAAGAACTTAATTCGGTTTTTTGATTATTGTAACTTTCTATAGATTCTGTTCTTTGAGTTGAATAATTGTCCAATGAACCTAATAGTTTTCTTTGTGTTTCATCGATACTATTTTTTGCTGCTAAAATATCAGAAATTTTTGAATTTTCGTTTATTTTGTGTTTTTTAATTTCTTCATCAATTTGTTGTTTTATTGATTTTATTTCATCATCGATATTAATAAATTTATCTAAACCCTTGGTTTTAATTATATCTTCTAGATGTTGTTTTTCTGTTTTAAATTCTTCAAATTTAGCCTTTTTATCTTCATTAATTTTTTGAATATCTTTAGTTAATTTATCTATTGCTGCTTGCAATTTTTGGTTAACTTGTTCAACATTTTCAATAGTTGAACTATTATTTATAGCCTTGGCATTAGTAGAAGCTTGATCTTCAGAAGATAAATCAATATTTATGGATTTTGCATCATCTTTAAGATTTGCAGTGTTTAATAAATCTTCAAGTTTTGGCTTCAATTCATAAAACTTATCTAATATTGCCTTGTTGCTTCCAATTTCTGTAAATTTTGCATTTAAATTATTTGTAGCATCTTTAATTTTTGAAATTTTGTCGTTTGATGTAATGTTTTTATATTCATCAATAATATTATTAAATTTATCTTGAATAATCTTATATTTTGAATCTTTAATATCGTTTGTAATGTAGTTTTGCAATGTTGTTTTAGACAATTTAAACGCATTAAACAACTCTTGTTTTTTGTTTTTAATTTCTTCTTCTAATTTTGTTTTTGCAGTTGTTAAATCATCAGTAGCTTTTTTAATTTCAGTGATTTCACATGAATCATTTATGCCACTGAATTTTTCTAATTCTTGTTTTGCATCGTTTAAATTTGAAACTAATTCATCACTTTTAGCATCTTCATCATTTTCAATAAATTCTTCTAATTCTTTTTTAGCTGAAACAAATTTATCGATTTCTTGTTTTTTATCATTTGCTTCTTTTAAAGAATTTGTTAGCAAAGTGTTTGCTGATTCAATATCTGATTTATTTGAAGATTCAGAAACTGAATCTTTTTCATTTTTAGTCGTGGTTAATTTTGAAATTATGTTTGAATAAGTTGAATTATTTTTATTTGAATCAACAAATTCTTGAATTTGACTTCTTGTTTGTTCTAATTCAGCAAATTTAGCTTCTTTTTCTTTATTGAATGTTTCTAATTTTTTAGTAATTTCAGCAACTTTTGCATCTAATGCTTCTTTAGTTGATTGCATTGAAACATTATCGTTTGAAGTAACTGCTTGATTTGCATTTTGAATTTCTTGATTTAATTCAGCTTTTGCTTTTTGAATTTGTTGGTCTGAATCAGCCAATTTATTTAATAATTGGTTTGCATTGGCAATTGAATCATTTAATTGTTCTTTAATTGATTTATTTGCATCATCAATTTGTTCTTTTGCTACTTTTGCCTTTTCAAGGGCTTGTTTTAATTCATTATTTGCAGCAATAATATCTGATTTATTTGAAGATTCAGAAACTGATTCTTTTGCTTTTTTAACATTAGCTAATTTTTCAATCAAATCTGCATAATTTGAATTATTTTTATTTGAATCAATAAATTCTTCAATTTGACTTCTTGTTTGTTCTAATTCATTGAATTTATTTGTTTTATCTTTATTAAACTCAGTTAATTGATTTTGAATTTCAGAAATCTTTGTATCAAATGGCTTTTGCAATGCTTGGATTGCAGCTGTATTGTTTGAAGCAATAGCACTTTCTGCTTTTTGAATTTCTTGGTCTAATTGTGTTTTTGCTTGTTGAATTTTGCCATCACTATCAACTAGTTTTTTAACTAATTCTTTTGCAGTAGTTAAACTTGTAGATAATTTAGCTTTAGCATCGCCATTTGTTCTATCAGCTTCAGCTTTAGCAGATTGGGCTGTTTGCAATGCTTGTTGTAATGCTTCATTTGCAGCAATAATTTCTGATTTATTTGAAGATTCAGAAACTGATTCTTTTGCTTTTTTAGCATCAGTTAATTTTGCAATTAAATCTGCATAATTTGGATTGGTTTTATTTTCATTAATGAATGCTTCAATTTGACTTCTTGTTTGCTTTAATTCATTGAATTTAGCTTCTTTTTCTTGATTGAATTTATTTAAGATTTCTTTATATTCAATAAGTTTAGTATTCAATAAATCATTGGCTGATTCCATTGATTCTTTGTCTTTTGAAAGAGATTGTTCTGCTTTAGAAATTTCATTATCTAAATTAGATTTTAAATCAACTATTTTTTGGTCATTGTCAATTAATAACTTTTTAAATTCTTTGGCATTATTTATAGTTTCTTTAAGAGTATCTTTTATATTTTGACCATCTAATTTTTCTTTTTCACGGCCTGCTTCTTCTTTTGCTTTAATTAAAGCATTTTGTCCAGCTTTTATTTCTTTAATTGTTGAATCATCATTTATTCCATTTAAACTATTTATTTTATCTAAAGCCTTTTGTTTTATTGATTGATATTTTGCATCATTTAATTTTGTGTTAATAAAATCTTGTAGGTCTTTTTTAATATTTTCAAATCTTTGAAGTTCTTGTTGTTTGGTATTTAAAATTTTTGATGTTAAAGAAGAAGTTGCTTTTTCAACTTCAATAATTTTGTTTTTTATATCTTCAATTGAAGATGCTTCACTTATATTTGTTTCAATATAAACTTTTTGTTCTTTTGCAAAATCTACTTCTTTTGCATCTTCTAAATCAAGCACTCTCTTTAATTCTTGTTTTTTATCATTAAAAATTTTTGATTGCAATTCTTTTTCAGCGTTTATTTTATTTAGAAGTTCTTTTTTTGCATTTTCAAGATTTTCATTTGTCTTTTTAATTTTATCTATTGGCAAAGAATCATCTATTTGATTTTCATTTAATGCTTTTTTAGCATCAAAAGAATCGACATTTTGACCGTCATTTGAATTAATTAAGTTTTGTAAATCTAATCTTAATTTATTTAATTTATTTAATTCTTCTAATCTTTGTTTTTCATCTTTTTGTAGATTTTCTAATATTTTTTTACTAAAAATTTCTATTTCATCAATAAGTTTTGATGTGTTTGAATGATTTTTAAATGCTGTACTATTAAATTTCTTATCTTGTCTTGCAATAAAATCATTTGCTATCTTTTGACCTTCATTATCTAGTTTCTTAATTAGATTAAGTAATCTCGATTTTGCTTCGTTATACTTTTTTGTGGAGTTATTGCTTATTGAGCACATAGCAGCGATAGCTGATGTTGATGCAGCAGCACAAACGATTGAAATTGACATTAAAATGTTTATTCTTTTTTTCATAATTTTTTATTATCCTTTCAAAAGCTATATTTAAATAATACAAATAATAATTATTATTATATTTTAATAGCTAAAGAATTTGAAATTAAACAATTAATTAGTATTTTTTTAAACCATTAAGTGCATTAAATTTGTTTTGCAGAAAATATTTTAAATTAAGGAATATTTTTTATCTTTTTGACCACTAAAAATATGTAAAAAATATCGTTTTAATTTTTGAAATAAAAAAATACTTATAAAAGCATAATAAAATAATGGTTTTAATATAAAATTAAGTAAATATGAAAAATCATTCTAATACTAGACGAAAGCATAGATCATTGGCGCCCTTATATTATACCCTTGGCGCAATTGCAGCTGCTGCTACAATTGCGGGGGCTATATATTTTGTAGTAAAAAACGGTAGAAGAGTTAGCAAGCAATATTGAAGTGTAGAAAAATTTTTGCAAATTAGTAAAAATATAAAACTTGATCCGGTTGTAATTAATTCAGCTTCTGCAAAAGAAATTTATGAAAATTTTATTCATGAAAAACAAAAAGTAGATGCGTTAAGAAATAAATATATTAATCAACACCCTGAGTTGAAACCATATTTGAATAATAATACTAGAACGGCATTTGATTTTAAATTTAAAGGTCCTAAAAAGTCTGAAATTAAAAAATTACTAAAAGATATGCCTGAAGAATTTGATGCTCATAATTTCATCTTTTCAAAAATTTTGACAAATCAAACATTTACTGAAATGAAATTCTTAAAAATGAGATTTTCTAATTTAGAATTAGACACATCTGATAAAGAATCAAATAAATTAAAGGTAACATATGAAGTTTATTTGAATTATGAATATGCAACGGGAAGCTTCGAACTTAATAACGTTAAGGGCACTTCACAATCTAAATATTATTTTAAAGGAAATCAAACTGTCAAGGTAATTAGTGATTCTGAAAAGTGAAATTTGAATTCTAAATTTGCTAATAATCTAGAAAAAAATTTAGATGATGCTAGAAAGATTATAAGAATTGGAATAGTTAAGAATGATCCAAAGTGAACACAATCAAATGAATTTAGAGAAATGATTTTCAAATGATTTAAAAGAATTTTTATAGAAAATGATGCCTTTCCTGACACATATAGCAAAGATGAATATGAAATAATGCCTTTTAATGAAACAAGTAGTGGTGATGATTATGTTAGATGAAATCCTAAGAAAGGATTAAATAATCTAACAATTAAGTTTTTTTATGTAAATAAAAAAAATCCAGATATTAAATCACCTGGCAGGATTAAGATTTTTACTATATTAAAGGTTTAGAATGGAAATAATTAATAAAGATCTAGAGGCAAAATTAAAAACGTTTCAAAAGCAAAATTATAAATTGCAAAAAATTTGTCAATATTTTAAAATAGATTTAAAAAAACTTAATTTAAATGAATTTTTAGATTTTAATAAAAATAGTACAAACCCCTTGTTGGATTTAAAACAAATCAAACAAATTATTGCAGAAAATCAAGGTGATTCTAATATCCTTGAAGTTTTAAACATGATTAAAGAACTAGAAAGTATTAATAAAGAAAAAACTGCAAATGATGAAACTTATAAACAAGATCAAATTTTAAAAAAAATTTTAAGTAATTTAGAGTAATGTGTCTATAATTAGTATTAATTTAGTTATATGCATCAACACGCTACAAATGTAAAGAAAGTAGGTTATTATGAAAACACAAAAGCGTATTAATATTGCAATTGATGGACCTGCTGGTGTTGGCAAAACTATTATGTCAACAATGCTAGCAAAAACATTAGGTTATAAATTTTTAAGTAGTGGTAGTTTTTATAGAGTTGTTGCCTACAATGCATTACAAAATAGACTAGATTTAAATGATGAAGAATCTATTAATAATGCATGAAATATAGAAGATATTCATGTAACAGAAGACGATAAAATTATTTTTAGAGGTCAAGATGTTAGTAAATTAATTAGGCAAGAAGAAGTTTCTATGGCCGCATCAGCAATAGCAAAATTTCCTTTAATAAGGGCAAAGGTTAATAAGTTTATTCAATCATTTGGTCTTAAATACAAAGGAATTATTGTTGATGGTAGAGATGCAACATATAGAATCTTGCCGCAGGCAGAGGTTAAATTCTTTTTGTGAGCTACACCTGAAGTAAGAGCAAATAGAAGAGTTGCCCAAGATTTAGAATTAGGTTTGAAAGCCGACTATAATGAGGTATTAGAAGATATAAAAATAAGAGATTATAATGACACAAATAGAAAATTTGATCCATTAAAAATTAGCGAAGGCAGCATTGTTATTGACACTTCAAATATGAGCATTCAAGAAAATTTTGACGTAATGTATAAAGAAGTGCGAAAGAGGCTTGATGATGAGAAATAATATTGTTGCAATAATTGGAAAACCTAATGTCGGTAAAAGTACCTTATTTAATAAAATCATAAACAAAAGAAAATCCATTGTTTATGATACGCCCGGGGTTACTAGAGATAGATTATATGAGGATGCAATTTGAACTGGCAAAAAATTTAGAGTAGTTGATACTGGCGGTATAACTATTGAAGATGAAGATTTTAAAAAGCAAATTAAACTTCAAGCACAAATAGCTATTGATGAAGCAAATGTAATTGTTTTTTTAATAGACGGCAAAGAACCGCTTACAGTTGAAGATTATTATGTTGTTAGTTTATTAAGAAAAAGCAATAAACATGTTTTATTGGCAGTAAATAAACTTGAAGGTTCAAACGTTAATTATTATGATAATTCAATTTACTCGCTTGGTTTTGATGAAATATTTCCAATTTCAGCAATTCATGGTGATGGAATTGGAAACTTATTGGATAAAATCATAGAAAAATTAAATTTTAGTGATAAAGATGTTGAATCATATTTTAAATTGGCATTGCTTGGAAAAACCAATGTTGGTAAAAGTACATTGCTTAATACTTTGGCTAATGAAGAACGTTCAATTGTTAGCAATGTCGAAGGGACAACGCGAGACTCTGTTTCATCTTTAATAAAAATTAACGGAGAAATCTTTGAGGTCGTAGATACAGCAGGTATTAAAAGAAAAAGCAAACTAACTGAAAGTGTTGAACATTATGCTTTAATGAGAGCAAATCAATCAATTGAAGATGCAAATTTATGTCTATTGGTTTTAGATGCAACTGAAGAAGTTAGCCATTTTTCTCAAAATGTAATTGGCATAGCCTATGAATTAAAAAAACCATTAATTTTGATTGTAAATAAATGAGATTTAATTGAAAAAGATACAAACACAATGGAAAAATATAAAAAAGACTTGTTAAAAAAAGTTAAATTTGTTGATTGATCTCCTATTGTGTTTATATCAGCCAAAGATAATTTAAGAATAAATAAACTAAGAGATACTATTATTCAAGTCAAGAATAATATTTCAAGAAAAATCAATACAAATCAATTAAATGACTTAATGGCTACTGCTCAAATGATAAGACCTGCTTCATCAATAAACGGCAGAAGATTATCCATTACATTTGCAAGACAAATAGATGGATCAATTCCAACATTTTTATTGTTTGTAAACAATGTTGATTTAGCCCATTTCACTTATCTAAGATATATTGAAAATCAAATAAGAGAAAATTATGATTTCTCTGGAACTCCTATAGAGCTAGTTTTAAGAAATAAAAACAAAAAAGAAGAGAGAGAATAACAATGGCAAATATTTCGATTTTTGGAAGTGGCGCTATGGGTACTGCCGTGGCAACTATCTTAGCAAATAATGGTCATAATATTACAATATATGGCATAGATGATAGTGAGTTAAATGATCTAAAAAATGGTAGAAATACAAAATATTTTGAAGATGTAAATTTACCAAAATTTAAAGTTACAAAAGATAAAAAACAAGCGGTTGAAGATTGCAATTATATAGTTTTTGCAATTCCAACTAAGGCTTTGCCTGAAACATATAAAGAAGTAATTAAATTAATAAAAAAACAAGTCATAATAATAAATGTATCTAAAGGCTTTTGAAAAGATTCAAATAAATCTTGCCACGAAATAATGACGCAAGAAGCCCAAGCTAATTCATTAATAAGTGGTGTAGTTTCATTAATTGGGCCATCTTTTGCAATAGACATAATTAATAAAAATATAACTGCCGTTGACGCTGTTTCTAATAATGATAAACTAAATAAAAAAGTCCAAAAATTATTTTCAAATGATTATTTTAGAGTTTATACGCAAAAAGATGTGGCAGGAGCAGAATCAGGATCAATTTTTAAGAATATTTTAGCGATTGCTAGCGGTATGGTTGCTGGGCTTGGATATAGCACAAATAGCCAAATAGCCTTAATCACTCGAGGATTTAATGAACTAAAAAAATTTGTTTTAGCTCGTGGGGGTAAGCTAAAAACGATTTTTGGATTGTCGTGCCTGGGTGATTTAATGCTAACAGCATTGTCGGATAAATCACGAAATTACACATTTGGAAAAAATTTTTATAACAAAAATTTTGATAGTTCAAAATTAACAGTGGAAGGTCTTAGATCAATAGAAATTGTTTACGATGAATATATAAAAACAAAACTATTGGATTTGCCTATTGTAAATTCACTTTATCAAATAATATACAAGAATGCTAACCCTGATGAAATAATAATTAATTTAATGAAAAGAAAGCTAAAATCAGAATAACATAAGGAATAACAATGAACAAGAAAGAACTACTAAGTAAAGCTAGCAATGAAACAGGATTTGCGCATGTATTAATCGAATCTGCATTTGATGAAATAATCAGAATAATTGCCGACGAAATAGAGGCAGGAAGAGAAGTTACAATTTCAGGATTTGGAAACTTTTCTTCTAAATTTATAAAAGGCAAGGAAAAGGTTCATAACATTACTAAAGAAATTATTAATGTTCCAGATAGATTGGATCCACGTTTTAAATTCTCGACAGCTTTTAAAAAACGTATTAATAGTAAAAATTAACTTAAACGCCGTTTATGGCGTTTTTATTTTAAAATATTCAAAAAATCTAATATTCCAGGGAAAATAAGTTTTATTTCTTTGCCTAAAGATTTTAATGCTTCAAAACAAATGTAAGATTTTTTTTCTTCAAATAGACACTTTTTAAAATTGGCATGTTCAATCAATAAAAACTCGTTTTGAAATTTGAAATAAATTATTCAAAACGCAATTCCTTGATGCAATAAAACCTTATTTAAATATTCAATTTGATGCTTTTTAATATTCTTTTTTTCAAAATTTTTTTGCTCCGTACTTTTAACTTCGAATGCTAAAAATCTTCCTTTTCAAACTCCATAATAATCTACAGTGCTTTTTGAAAATATGCTTGCATTGTTTAAAAGTAATTTATTTTTTTCTAATTTGACATTTTTAAATTTAATATCTAAATTTTTTTTATGGATTAAAGCAATTCCATTTTTAAAATAATACTCATTAGTGTTGTTTATTATAGATTCTAAGAGCATGCCTCTATTATTTTTATTTTTCATGTAAAAATTACAACATGTTAAGCCTTAATTTTTTCGCTACTTTTTCGCCTTTAAGTCTTTATATGTTTCGAAGTTTTTAAAATAAATATTTTTTGATTTTAATAAATATTCAACTAAGTCAAATGCCATAATCATTGCCAAAACATCGTTATGGCAATATCTTTTTAATTGAGCTTCTTTGACCGCCCATTCATTATCTCTTATTTTATTTAAAGCTCTTAGCGTTGCTATTTGCAATGCCATTCCACCATTTTTTACATCAAGTTCGTGATATGGAAAAATTTTATGTTTTAAATTTAATTTATTTTCATTTACAAAAAGTTCAATCTTTTTTATTGAATAAACATTTTTTATTTGCCCTAAATTTACAATAGAGCTTTTTATATTGCAATCCATTGCTGGTCTAAAAAATAAATTTTCTATATCAATAATCTTGCTTAATATAAATTGAGATTTTTCTTTTAAAATGTCAAATTCATTTTGGCTAATAAATCCATCATCTTTGTAATATTCCAAAATTTCTTGCATTTCTTTTATTCTAGATGCTTCATATGTGTCGTTATATACGACATAATAATCTGCTTTCTCATCATATAAATCATTTATTACTTTCATAATTGTTTTATAGTTGTAATTCAAAGGGTCATAAACAAAATCATTTGAACTATATATTTCACCGTTTTGAGTTTTTATTATAGATGTTTGACTTATTAATTGATTTCATGCTAGAGAGTGGTTAATCATTGGAACGGGCAAGGTAACGCCTTCAAAATCGAATCAAACTATTTTTTTATTAAATTCTTTTAATAATAAATATTCAGGGCATTTATCCTTGTTTAAAATATCGGGTGAAATTGAAATTAAATTGTTTTCATAAAAATCTAAAATTTCTTTTGTTTCAATTGAATTATCAGTTAATGATTTAACTTTTAATTTTAATAATTCCTTTTGACAAATAATGTATTTAGGAATAACCTTTTTAACTAATAATTGGTAGAATTTGATTCCTAAATTTAAATTATCTTTTAAGTCATCCATTGTTATATTGCAATCTAGTTCGTGTATTTCTTTATTACTATCAAAGTTTTCAACAATTTTTAAGAAATCAGAAAAATTGCAAGTGTATTTACTTATCTTGCTTTTTGCATCGTTATCTTCAATTTCAAAACGACTATTAAAATTAAATGCATTATTTAAATTTAATTCATTTTTTACATAATCAATTATTTTGTCATTTTTTATTTTTTTATTATCTTCATTTAGCAAAGGATTTTTATCAAAAATAGCATTAATTTCAGTTTCTGATAAATTCTTTTTTTTATCAAATGTTGCTTTTGATTTTTTATAATTGCAATATTCAGTCTTTATTATTTTTAATTCATTTTTTTTCGAGTTTTTTCTTGTTTGATATCAAGGTTTCACAATGAAAATTTTATTTATTTCAATTCCCGAGGATGAAAGAACAGAATAATCATAATATGCTCTTAATAAATCTTTTAGGCTGGTTTTTGATGAATATACAATATTTCCCATTTCTTTAGTTTTACTATCGTAGTAAAAAGGCTTTGATATACATTTTTTATATTCAAAAACAGGATTTATTATTAGTGAATTTTGCATTAAAAATTCTTTTGTTTGTAAAATTTTTTCATCGGTTGAACATTTTTTAGAAATTATATTTACTTTATTTGAAGAATTTTTTGTTAAAAAATTTATAATTTGTTCCTCGACAAGGGCTATTCCACTATCAACCATTTGAAATTGAAAAAAATTATCCAATATTTTGTTAAATAATTCTAGTTTACCATCTGAATTAATTTTTTCAAACAGTGTTGAATCATTTTGCATATCTTGTTGAATTTTTTTTATTTTTTCGATAGTATTTAGCTTTAAAAAGTCTTTGTTTTTAGTAGAGTTATTTTCAAAGTATTGATTAAAATTTTTGTCAAGAATGTTTTCAATATCATCATTTGCAAATTCCAACATTTCATAACTATTGTCTTCTTGGTTGTCGCTTTCGTAAACCTCACTAAAATCATTCAATAATTCTTCTTCTCAATTATTAAAGGCAAAGTAAGGTCTGGCGGTATTTAGAATCAAAAAATTTTTATAATTAAAGTATTTAGGCTTGTTCATATTCGGGAACAGCCTTTCCTTGATCTACTCAATATAAATATAAATTTTTCCATTCTTCCTGGTTATTACATTCAAGGAATTTTAATAAATCTTGGTCAATTTGTTCATTTCATTTTGGACTTATTCCAATTTGAGTGTATTTCTTATATCATTGCATACAATAATTAATATCGTCTTGGACTTGTTCGTTGTTTAAGATATATGTTCAATTCTTTATTATTCTTTTAGTTATGTCTACGTCGTTTGGATTTAAATAATCTTCTTCTTTTAAAAAACAAGCAACGATACTAAATGAAGTTGCTCCAATCAAGTATGTGTATAATTGAGCTTGCTTTATATAGCCAGGATTAACTCCATATTTTATTCAATTTTCATAATTTTTTTCCCCGACAGTTTTAATTTCAATAATTAAATGTCTTTCGGGACTATAGCCATCAGGCAACCCTCCAAATAGCTCGTTATCTTTAAAATAATCATAATTATATTGATTGGCTTCATATCTAAGCAATTTGGAATTTAATTTTTTTTCAATAATTTTTACTATTTTTGGTTCTAATGCAATACCTGCATCAACATATTTCCTGTTCAATATTGGTTTTCTAAAACCGCATATATTACAAAATGCTTCAAATTGGCTGTCAAATGAGCTCAATCCCATTATAGCCCCCAAAGCCGAACCTGTCATTTTTTTAAATCCTCCAAAAGTACCAGGTTTTTTTTCTTGCATAGATTTTAAGATTTTTGGATCTACTATTAAAGTCTTATTTTTATAATCCAAAGTGTAATTTTTATTGTTAAAAAATTTTCTTTTAGGAATTATTATTTTTTTTTCCATATTATTGCCCTTCTTAATTAATTACTATTAATGTAATGAATATTTAAATTATATATTTTCAATAGTTAAAAACTAACAAAATGGGAAAAAATAGTGAAAATATAGTTTTATCAAAAAAAATAAACAATTAATGTTTAATTTAAAGTAAATAAAAAATGTTTTTTTTATTAATGTTCTAACACTTAAAATTTAATTAGTATATAATTTTTTTTGTAAATAATGAAAAGAGGAATGATGAAAAAATACACTAAAATTTTATTAACATTAGGAGCTATTTCTACTTCTATTGTTGCTGTTCCATTAGTAGCAGCAGGATGCAATGATCCAAAGAATAAGAAAAACCCAAACAATAATGATCAAAGTCAAGATAGCCAAATTGAGTTTCAAGAAGGCTTGGGGTTAAAAATTGCAAAAATTGCCAAAGATCAAGAAAATGTTAAAGTTGAAGAATTTGTAAAAGAAGCAAAGAGTGCAAAGACTCTAGATCAAATAAAAGCATTATTTACTAAATATAAAATTGCTTATGATTTAAGTGAAATTCCAGATGATGCAAAATATGAAGTTGCTCCTGGTACACACGGTCATGCCGATGAAGGACTAGTACACTTAGATATTATCCAAACAGTGAATGAAAAGCAAAATAATAATAGATTTGAAATTAAAGGTTTTAAAAAGGAAACTGTTGAAGACACAGTCCAATTGGGAGGATATAAGTTATCAACAAAAATCCTAGATAATAAAAAAGATTTTTCTTTGCAAGAAATAAAGAAACAAATTATTGACGCTCAAGAAAAAGGCTTTAATTCTTTACTTGAAAAATTAAAAGAATTTGTTAATATTGAAGTTGATGAAAATAATAAAACGAAACAATTTGAATTTAATAAAGAAAAGATTGAATTAATTAGTCAATCTGGTCAAATTGTTTTTAAAGGAATTTCTATTATTGATATTAGTAAAAAAGTAAATGAAGTCGGATATAAAACCGAAAGCAAAACTGATTTTATAATTCAAGGCTTGAACAAAGTTGAATAATTAGTTTATAATTAAGGCAATAATGAAAAACAATATTTTAATTTTATTTAAGCACATTATTGTTTACTTTTTTGTCTTGTTTGTCATTGTTAGTATTATCTTTTTTATTATTAACTCATTGATTGAACCACAGATAAAAAAGAATATTGCAGTTTATTCACAGATAAATTCAAGCCCATGAATTAGATATGGCGAATTTTTGAATAATTTGTTTTCATTTAGAGGTGGCAAGATTTATTCAAAAGAGCTTGCAGCAACTAATTTGTCAATAATTACATTGTATTTTATGCAATTTAAATGAACAATCTTATTTACTGTAATTATTTTTATTTTTTCTTTGATTATTGGAAATTTACTAGGAATTTGAAGTGCATATAAATTTAATAAAGCACCCGATTTTGTCGTAAATATAATTGTTAGTGCTTTAGCTACAATACCTTTAATAGTTCTTGCAATATTAGCATTAGGAACATCAATTTTTCTGGGATATCCAAGTCAATTTATAAATAATGGAAGTTTAACTTTTACTTCTATAATTACACCAATTATTATTACGGCATTTGGCACAATTAGTTTATTTCATGCAAGATCAAGAAAAATAACAAAAGAAACTCTTTCTTCTAATTATTATTTGTTTGGTATTTCACTAGGAAATTCAAAAACAAAATTATTTAAGCAAAACATTATTAAAAATTTAGTTATTGCTGAATTACAAGTCATTTTGCCTTTTTATATATTGCTTTTTAGTACTTCATTAATAATTGAGAGAATTTTTTCAATACAAGGACAAAGTATTTTTATTTCTTATGTATTTACAAAAGCAGAATTAAATTGCATAATGTTTTATTTTAGTTTTAATTTTTTTGCTTTATTGATTGCTAGATTCATAAATGAAATAATTCTAAATTATTTAAATCCTGCTCAAGCTATTGAAAAGCGTATAAGATGGATTAAAAGAAAAAGGTTAGATTATGAATGAACTAGATAATAAAAAAGAACTTTTTCAATTTGCTAAAAAAAGACAACAAATTCACAATTACCCTATATTTCAAAGCGAAACAAAATTATTTTGGAAGAGATTCTTTAATAAAAAGAGCAACATCGTAATATTTAGTTTTTTATTAGCATTCATTTTACTAACATTAATATTACTATGTTTTGTAAAATTTTCTCCAATACATTCGATTGATAATAAAAGCGATTTTGTCAATAATTTACCTCCCTATTATTCTCAAATTATTACAAGAAATTTTGAAAGAGGATATCAACTTGATTTGATAAGATCTATTGAAAATCAAGAGTCAATTAGGGCTGCTAAATTAGGTATTAAACCAATATTTAGAATCTTGTATGATTCAGCAAGAGATAGTGGGGGAAGTTTAACTGTAAATACTGATATTGTTGCTTTAACATATAATCCCTACGATTTAATAAAGGCAATAAATTTGAATCAAAATTCAATAAATATTAATATTTTAAATAAAGTTTTACTTGGAACAACAGAAAGCGGAATTGATATTTATTCAAGGATATTTTGTGCATTTGCATTAACAACCTCAATTATATTATTGGCAATATTTATTAATATTTTTATTGGGTTTTCTCTAGCAGCATTTTATGTTTTTAATCGTTACAAATGATATGGAATTGTTATCGATAAAATTGCTGTTACACTTAATGCAATACCTGAAATAATTTGAATATTTTTGTTGTGCATATTTTTGGGTACAAATTGATATGGTATTCTAATTGCTTTCATATTGATTAGTTGAATAAGTTTTTATGAACTTTCAAAAAACGAAATTCTTAATTTAATTCAAAAAGAATACATAAAAGCAGCTAAAATAATAGGTTTAAATAATTTTCAAATTACTTATAGACATTTATTTAAAAATGTCCTACCTTCATTAATTATTTTAATTGCTGATCGTCTTGCAATAAATATTCTAATTGTTGCATCCCTTGCCTTTCTAGATTTCTTAACTGCTGAAAGTAATTTAAACATAGGAACAATTTTTAAAGAAGCATTATCTTCAATAAAAGTAAATCCTCAATACTTAATAATTGTTGCTGTTTATATTACTATATTTTGTATTTTACTTAAACTTTTTTCAATAGGTCTTTCAATGACATATGATCCTAAGATTTCTAAAAATTATTAATAGAATTTAATTAGTTTTATAAAATTCTTGTTATATATCGTTAATTATTTAATTTTAATATACGAAGCTAGAATTTTTATAAGGAGTTTAATTATTATGTTGAATTTTATTTATAAAAACGATTGCAAAATCATATTTGGAAAAGAATCAATTCAAAATTTAGAAGAAGAATTGAAATCTTTAAAAGTAAAGAAAATTTTAATTGTTTATAGTGGAAAATATATTTTTGAACTAAAAATATATGACGAAATAATTAAAGCATGCAAAAAATTAAATATAGAATTTATTGAAAATGGTAATGTTGTTCCTAACCCAAGAATTGAATTAGTAAGAAAATTAGTATTAGAAACAAAGGCTCAAAATGTTGATTTTATACTTGCCATCGGTGGTGGTTCGGTAATTGACACCGCCAAAGCAATAGCAGTTGGAGCGAAAAGCGATGTTGATGTTTGGAAATTTTATACATATGAAGATAAACCAAATTGCGCATTACCTATCGGAGTTATTTCAACAATTGCATCTAGTGGTTCTGAATCGTCAAATTGCTCAATTTTGTCAAATGAGAATCACAAACTTGGCATAGAATATGATTTTATTATTCCAAAATTTGCAATAATTGATCCTTGATATACAAAAAGCCTCCCTATGTATCAAATTTCATGTGGCATATCTGATATTAGTTCTCATCTAATTGAAAGATATTATACAAATATAGAACATGTTGATGCAACAGATTACATGATTGAAGGACTTTTAAAGGCATTGATGATAAATGCTAAATTATTAATGGAAGATCCAAATGATATGAATGCAAGGTCAGAAGTATTTTTAATTTCTATTTTTGCACACAATAATATGTTAGATTCTGGTAGAGTTGCTGATTGAGCTTCACATAGAATTGAACATGAATTAAGCAATTTTTATGGTATAACTCATGGCGAAGGTATGGCGATTGTCATGGTTGCATATGCAAAATATATGGCAATTGAAAAACCTAAAAAATTAGCTCAATTAGCCGAAAGAGTTTTTAATGTAGACAAAGGTTTGAGCCAAGAACAAAAGGCTAATTTATTGTCAAAAAAATTAGATGAATTTTATAGAACAATAAAAATGAGAACTAGATTATCAGAATTAGGAGTAAGTTGCGAAAAGTTTCGTGAAATGGCATTAAGTGCAACTAAAAACGATAGTCAAACCATTGGTCATTATAAACCCCTTTTGTCAAAAGAAATAATTGAAATTCTAAAGATTGCAAAGTAATAATTAATTTTTATATTTTATAAAAAAAACATGCCTTTTTTAAAGCATGTTTTATTTTTTTACTATTGAAGTTAAAACTTTTTCTACAATATACTCTTCATCATTTTTTTCAAGGTTGCAAAATTTATTTAATGCAATGTTATATTTAGCAATTTTTTCATGAGATAATGCCTCATAAGATTCAATTAGTATTGAATTATTTCTATCGTAGTTCAGGCTTTCAATGTTTTCAATATTTGATATTGAAATAATAGAACTATTTAACCTTCTAGCAATAATCTCTGCTATTCTAAGATCAGATCCTGCTGCTATAATATTTGTTGTTTGACTAATTTTTCTAATGTAATATGGGCCATCTTCAAATTCAACTTTTGAAGTATCCTTTATTTCTTTAATTTCTTTATTTTCTAAATAAATTATTGATGACTTTTTTGTGTGATTAAAATGAAATTCAAACGCTCCTTTTAATTCATTGAAATCGCAAGGCCTCAATAAATTTATATGGTCATATGAATATAATATTTTAAGCTCATTGCTTAGGCTAAAATTATTTCTATTATTGATTAATAGTATAAGAATTTTTGCATTTGAATATTTTAAGACTTGCAATGTTGGCAATAAATAACCTAAATAATTAATATCTGCAATGACAATTGTTTCTATGCTTGAATGCAAACCTAATCCCATTGCCATATTAATCATTGTAAATTCTCTATTACCTAAGAAAATTCCTCTTCCAAGATTGTTACTTGCAAAAATCCCATTTAACGATTTAATATTTTGAAAATAAGAAATATCATTTGATAATACTAGTAAATTTTTGTATTTATCACATAAATTATTTATTATTTTTAATGCATTTTCAATGTATGTTTTTTCTTTAAATTCTTCAACATCGTTAATATTTTCACGAATATTGTTTTTTAAAAAGATTTGCAAATTATTTGAATTACTAAATGTTTTTATTTTGTTTTCTATTTTTTGCAATCTATTTTCATAAAACTCAACAACCTCAGAATACAAATCACTCAAATTTGTTTTTTGAAAAGAGGTTTTGTCTTTGAATTCTTCTAATTTTTCATGAGTTATTGAATTATCAAAGTTCTTTTTAAATAATTGTTCGCTTTGAGGACTTAAAATTTTAATAAACACAGGTTGATTAATTTTTTGTGCTTTTTTTATTGCTTTTATTATTGAACGACATTGACCATTTGACACTTCAATGTATTTGAAATTCATCGACTCAACTTTTTTTGCTATATTTTCTGGATTAATACTTTTACTATCTACTGTGTATTCAATTTCATTTGCATCATAGATAACAATTAATTGCCTTAAATTATGAGTTCCAGCATATGCTAGTGCTTCTTGAGTAGATCCTTCTTGAAGTTCACGTTCAGTTACATATCAATAAGTAAAGTGATTAATATTCTTATCTAACCTATTTAAATAATTTTGAGCGATTGTGTTTCCTAATGCAAAACCGCCTGATATTCCTAAAAATATTTCGCCGCTTTTAATTCAATCTAATGTTCAATTCGAATATGAAATGTTTTGATTTCTATTGTTTATTAAGTTAAGGTATTCATTTTCACTAATTAAACTATATAATCTCAAAATTTCATAAAAAAGTGGCAATATGTTGGGATTAAAAAAAACAAATCTATCCCTATTGACATCAGAATTATTTTTTCTATTTATATTGAAAAAATAGCCAAAAAGAGCATGAAATATTTTTGCCCCACTCATGATTGAAGAAAAATCACTATATTTTCAACTAAATGCCATATTTAAGGAATTGATTTTTATATTGTCAATAGCTATTTGATCAATTTTTTTATTTTTCATCTTCATAACCTTGGAATATTAAATTAATATTGTTTGGTTTGGCATTTATTAATGAAAAAACTTTATGTTCAATCTTAAATATTAGTTTTTTTGTTTCATATGCAAAACTAGTTTTGTTTATTAATTTGTAACTTAAATTAATAAAAACATTTGTTTTAGTGCGATTTGGTTTAATTTCTATTTTTCCAATTAATTTTATATTTTTATTTGAACTAAAAATATTGTTTAATTCTTCATATATTGCATCTTGTTCAACAAAATAACGAAAGTTCATTTTTGTATTAACTTCAATTGCTCACATATTTTACGCTCTTCCAGTGTATTTACCAGTTTCGGTTGAAATAGTAACTATTTCACCTTCTTTTATAAATAATGGTGTTTCAACAACATAGCCGGTTTCTAGAGTAACCTTTTTTTGTGCTGCTTGAGCTGTGTTGCCTTTAACTGCATCTGGTGCTTCGACAACTTTTAAATTTACATTGGCAGGTAATTCAATGTCTAAAACCTCATTTTGAAATTTTCTTACTATTACCTTTTGTCCTTCTTTAAGAAAGTTTAATTCTCAATTAAGATGTTCCGAAGGTATTTCAATTTGTTCAAAAGTTTCTTGATCCATTAAAACTACAACGTCTCCTGCATTGTATAGATAATCCATATTAGATTTTTCAATCATTGCTGGTTCAACTTTTTCGCCACCAGTAAATGACTTAATTGTTGTCGAACCTGTTCTCAGATCTTTAACTTTTGCTTTAACATTTGCTTGGCCGCGGCCTTGTTTTGAATGTGCTGCTTCAAGCACTACATAAATATTGCCTTCTAATTTAAATGTAACTCCTGGTTTTAAATCGTTAACATTTATCATAACGTACTCCTTTAAATTTAATTAATAAGGTATTTAATCTATAAACTTAATAAATATAAATTATAATTAATTTATGGAATTTTACGTTTATTATTGTTAAATTTAATGATAATTGAAATAAGGTAGGTTAAAATGGAAGATTTATTAAGAAAAGTTCCTAAGACAAAAAAAGAATTGAAGATATATTCGGTATTAATCAATAAAATAGTTTCTTGATTAAAGCAAAAAATAAACTCCTCAAATTCTAATGGTATAACATTGGGGATATCAGGTGGAATTGATAGTTCAACATTAGCGTTAATTTCTGAATTAGCTTTTCCAAATGGTTGCAAATTTTATTATTTAAAAACAGAAAATGATTCATATACAGAAAGCCATATAAAACTTTTGCAACTTAAATTAAAGACAAAAATTGAAATTATTAACTTAAGTTCTTGCTTTAAAAAAATAGTAAAGATTGCAAATATAATAAACCAAGGTTCAATTGCAAATACTAAATCCAGATTATTTATGACAACATTATATGGTCTTGCATTTCAAAACAAAAATCTTGTGCTTGGCACAGATAATTTTGATGAATATTATTTGGGATATTTTACAAAATATGGAGACGGTGGATGCGATTTACTGCCTTTTGCTAACATAAAAAAATCTGATGTATATGCTATGGCGAGTTTGTTGGGTGTTCCACAGGAAATTATTGATAAAAAACCTAGCGCAACATTATACAAAAATCAATATGATGAAGAAGAATTAGGATTTAAATACTCAGATTTTGAAAAATATCTATTAGATCCAACATCTGTTTCTGAGGAATTAATTAAAAAGATAAAGAATATTCACAAGCAAACTGAACACAAAAGAAAAGCAATCCCCAAGGGTCCAAAATTAAAATAATGTTCAATATAAAAATTCAAGCATCCTTACCTAAATGCTTGAATTTTTATATTGTTTTTTATGCTTTTAAATTGTTTGCTTTGGTTCAATTGTCAAAAAAATTATTTAAGTTTAATGTTTTTATTGTTGAAATGTTTAGTTTAATTAATCCTTTGTTTTCAATATTAAATAAGGGGTTGTAAATATTCAAGGTATAATCATTAATTTTTTCATTTCACAATTTAAAATTACTATTTAACTTTAAATTGATAATGCCATTAAAGTGACTTGGAATGTAAAATCCTTTTTTGATTGAATTATTATATGATTTAATTTTTAAAAAATCTTGTGTTTCTTTGCTAAAAATATATGAATCATTTAGAAAGTAATTAAAAGTATAAGAAAGGGGTCCATTTTTTTCTAAAGTATATTCTAATTTGTTAGTAATATTTAATTTATTATTTTGACTATAAAAATTAATTGAATCAAAAATTGGATTTTTTAATTCTGGATAATCATAAATAATTTTTTTGTTGTGTTGGTTTGTTTCAATAAAGCTTTGCGAATATCCTATGTGATTTCAAGGCATATTTTGTTTCAATTGATTGAATTTAAAATATGTAACAAAAAACTCTTTAACTACAGTTGTAGAAGAATTATTAACATCTTCTTTAAAACTCTTGTCAGTAAGAAAAATATCAATATTTTTCAGGGTTCTTGTTTTAAAATCTGAGTCTATATTTCTATTATCTGATAGGTTATTTGCTAAGTCATAGTTAATAAGAATATTGGCTCCGACTTTATAGCTCAAATAATTTCTAATTTTTGTATAAGCATCAATTTTTATTATCAGTTTTTTTTCTAAAAATATAGGTTTTCCATCTTCATATTTATCAATTTTAAAGTGAATATATTCATCATTTGTTTTATGATAAATCGACAAATTTAGAGATTTTTTATTTATTGAAATTTCTTTTTTTGCAGCAACTTCATAAATCAATCTATGAATCGAGAGAGTGATTGATAATTTATTGCTTTTTTCATCTCTATTTATTTTGATAAATATTGGATATTTTTTTAGCATCGTATTAGATACTAAAGTATTTGATTTTAAAGATCTTGAAGCAGATAATTTAAAAGTTACATCTTCACAAATATAGTTTTTTGCTAGGATATCATTTGCCTTTTTTTCAACAACTACTTGGTACAATCTTTCTTTTCTTCTTTAAAAATATTTTCTTTGAATTCACAAATCAATCTAGTTTTTGCTATAAAATACAAATAATAAAAGGGGATTCAATATTTATATAAATCGTGTTGCTTCTTTTCTTTGTCTATTTTATATTTTTCCATTGATCCTCCGGTGTCTAGAATTTGCCTTCTAGACATAATAATGTAACCATGAAAATTAAGGATTTTGCCAAATTTTGGGAATTGTGAAATCTCTTATTTATTACATAAATAAAGAAAATTTATTGTCTAAAAAATTAAGAATCAAGCAATTGCTAAAATTAAATAATTTAATTTACGATAATATGATGATTTAGAAAAGAAAAAGTCTGTTCACTTAATATCATCTTTAAGTTCTTTAAAATATTTAACATAAATACATTTCAAATCATCACTCAATGAAGATTCAAACAATTTTGCTGTTTTTAAAACATTCTTTTGAATTAAAAATTTAAGTGAAATTAAATTCTCTTTATTAAAAATTTCAGGAATTTTATTTATATCTATTTTAATTTTTCGTTCCCTTAACTCTTTTATTATTTCCTTAAAATAATTAAAACATTTTAAAAAGAATGCTCTGTTGCTACTACGACGCATTTTTTGCCTCCGATTAAATTGCGTTTGTATGATTTTATATCTTTATTTTTAGTAAATAATTTCAAAAAAAGTTAAATAAAAATCTAAAAAAAAGTAAGTTATACAAAAAAAACTTACTTTTAGGTTTTGTATTTTTGATTTTATAAATATTTATCAGGATGTTCAATTTTCATTTTTTTAATAAATTCTTCTTTATCAAGAGAACCATATTTTTTCATTAATTCTTGACATTCTTTATATTCATTTTCGGCTCAAACAACATCTTCAAATCCTGTTACTAATGTAGCATTTTTACCTTTAAATTCTTTATAAGTAGAAAAGAAATTTCTTACTTGAACTAATCACATTTGATCGACATCTGCCAATGATTTAATATTATCTAATCTATAATCATCTGCGTGAACTGCAATTAATTTAGTATCTGTTTCGCCACCATCAATCATTTTCATTGCACCAATTAGTCTTGCATTTAATGATGTTCCGGGTAAAAAACTTTCTGAAGAATAAACTAATACATCCAATTCATCTCCGTCTCAATCTAGTGCATCAGATAAATAGCCATAATTTGCAGGATATTTAAAATCACCTCTTAAAATTCTGTCAACTTTGATTTTGTTGCTTTTGCGGTCATATTCATATTTAATTTTTGAATCTTTACTAATTTCAATGTTTACTTCTAAATTTTTCATATGCTTATTTTATCACTTTCATAGAAATTCCTTATTGTTTGGGAAAAAAATAAGAGTTTTCGCTTATTTTTCCCAATTGAAATAATAGTCAGAAAAATCCAATAAACTTAAAAGTATGAGGAGTAAAAATGAAAATATATTTATATGGAAAAATAATGCACGTAAACACTAACTATTTAATTTTAGATCATAATGGTGAGGGCGAATTGATATACGTGGCAGATATAAAAAGATTTAATAAGGATGATCTGAGAAAAATATTTATTTACGATGTGGTAAATGAGTATAAAAAAACTACTTATGGATTTGAAAATTTTAAAGAATTAATAATTTTTGAGGATTTAATTTCATTACAAGGTTTAGGACCTAAGACAGCAATTTCTCTTCTAAATTTAGGATGAGAAAATTTAATTCAACTAATTGCAAACGAAGATAAAGACAAATTATGCGAAGCGTCTTATGTAAGTTTGAGGATTGCAAACAATATAATTTTTGCCTATAAACAAAAATATGCAAAATTTTTATCAAAATTAAGCGATGAAGATTTACTTAAAATCAAGAGCAAAAACACAAGTGACTCTTTAAGAGAAAAATTTGAATATACAATGAGAATGCTCGGTTTTAAAAAACAACAAATTTTATACGCATTAGAAAAAATGAGTATAACCGAAAATATTGAAGAATCAGTTGAAAATGCAATAAAAATAATTGGAATGAAGCAAAATGAGTCAAGAGTTCTCGATCAATAGTTTTGATGAATTTGTAGGCCAAGAAAAACTAATTTCCACTATTAAAATAATAATAGAATCTGCAAAGGTTCAAAAAAAACAAATTGATCATTTATTATTTTATGGTCCGCCAGGTTTAGGAAAAACTACACTTGCTAAAATAATTTCAAAAGAAACAAAAAGTAATATTGTTTATGTTCAAGGCCCACTTATTGAAAAAAAGAGTGATGTATTGACTATTTTTTCTTCAATAAATGATAATGACATAATTTTTATTGATGAAATTCATGGCATAAACAAAAATATTGAAGAATTATTTTATTCAGCATTAGAAGAAGGAACAATTGATGTTGCATTGGGCGTTGATGGTGATAAAAAAATCATGAGAATGAAACTAAAAAAATTTTGTTTGATTGCTGCCACAACAAAAATCAATTTATTATCCAAACCATTAAAAGATCGTTTTGGCTATATAGGAAAATTAAGCGATTATAGCGACTTAGAAATTGCTAAAATAATTTTTAACTCTGCAACTAAAAATAAGATGAAAATTAAAAATGATGCGATTAATTTTATTGCAATTCATTCAAGGCAAACACCAAGAGTCGCTAATACTCTTTTAAAAAGAGTTAATGATTTTGCCATATATTACAAAATTGATCTAATTGACTTAGAAACTGTTAAGAAAGCCTTTTCGTATTTGGGTATTTATTTGTATGGTTTGTATTCGCCTCAAATTGAATATTTAAAAACATTACATAAAGTATTTAATGACAAATTCTCTTCGCTTGATGCGATAAGTTCAATAATTAAGGATGATAAATATACTATTTTAAATGATATTGAGCCTATATTATTGATTTATAAGTTAATTGAGAAATCACCCCGTGGTAGAAAAATTACAAACAATGGCATTGAATATTTAAAGAGTCAAAATATAATTACTTAATTACTAAGTAATTTTAATATTTTAGATAACAAGCATTTTTTAGTATAATTATTAACTATATGAAGAAAACAAAAATTTTAAACAATAAATTTAGATGAGTTATTAGCATTTTTTTAATTTTAATTTTATTGATTGTGACAATTTTAGGATCAATTTTTTATTTGTTTCCGCAACTTAAAAAACAATCCAATTCAAATGTTACATCTGCTAAAATTGGGTTAAAAATAGAAAGGGAAAATCAAAAAGAAAACTCGTATAAAAAATCATATGGATTATCAGATCAAGAAATTTTGAATTTGACTAAGAATTACATAGGAAACCAAAACGGGGTTTTAAGTACTTCATATGATATTAATTTGGTATCTAAAAATTTGATTTCTGCTACTAGTTATCAAGATAAAACAGATAAGGATGTTAATAATTTAATATCCAATTTTGTTAATAAGCCTTATTTAACAGTAACTGATGGTGGTGGTAATCCATTATTTTATAAAGGTGAATATGTTTCTAGGTTTTATCCAAATCCTGATGTTCCTAGAACTCTTAAAGATTTTTTGAAAAATGGACCTCAAAACTACAATATTAGCGTAGAAAAAAATCCAGCAACAACCTACAACAAATTTGGTGCAAGACAAAAAATTCAAGTTAAATTGGATGAATATGGATGGGATAGTTTTGTGCAAATGATAAACGAGTACACGTTTGCAACAAATTTATCTTTACAACGTAATAGAAAAGCCTCGCGTGAAGAAATTATAAATAACCCAGGCAATAAAATTTACTTTTGAATGAATTTGGCAGAATTCATTCGCAGAGCAAAAACCGAGTTTCCAGATGAATGAAAGGCAGCGGGAGAAAATCCTATAAACTTTGCTTATGTTGGAAATTCTGCTTTGCCTGAAATAAAAAAGGACAAAAAAGGGAATATCATTGATATTAAATATCCTGTTTTAAAATCAAAAGAAATTAATGCAAGAAAATATTTGATATCAGCAGTCAACCCCGCAACATCTAGAATACTTTCAACTGAAAAAAATGAATCGTCATTTTATTTAGCGAACGAAAATTTAGAGGGTTTAAGTGATGAATATATTGCTGCTGCAATTAACTATTCATATGCCCCATTTAAATTAACTAAAACCCACAACTATTTTTTAACCGGTAGTTCAACAAATCAAAATAGATATTTAGCAGTTTTAGCAATTCTATTTTCGTTATTTGCAGTATTTTTGATTGCTAAATATCGTTTATACGGAATTATATCTTCAATATGCTTGGCATTTATGATATTTGTGTTCTTATCAATTATTACTTCATTTAGCATATATATAACAGGAGCCGTGGCATTTACTTTCTTAGTTTCTACATTCTTAACATTTTTAATGATTTGACAAATATTAAGACGTTTAGATAAAGAAGTAAATTCGGGTTCTAACGGAATAAAAGCAACGAATAAATCATTAAGATTCGGAATAATATCGACACTTGATATTGCCTTTGCATTAATTGTGGTTTCAATATTTACTATCTTTATAAAAACTTCATATTTAAATGCGATGGGCATAATGCTATTTATTGGACTGGTTTCTGGATTAATTATTGGAGTATTGGTTAATGCGTTAATAATGTGAAATTTAATAAAAACGGAAACGTTTGATAAAAGAACAAATTGAATTGTTTGAAATAGTAAAAGAACAAATTCTTTGATAGCAAAAATTGATTTAATCTCAAAATCAAAATATTTTAGTATAGGTTTTGCAATATTTGTAATATTAACCATTGTATTCTTCGGAATATATAGCGGTGTATTTGGAAATACAAAATTAGGAATTAATGCTAGTGAAACATTAGCGAATAATTACTTATATTCTATTCAAAAATCATCAATAAATGGTGATTGAAATAAAACTGAAGTTGATAAAATTGTTCAATATTGAAAAGAATTAAATATTTCAAATGCAAATATTTTCTTTGAAAAATCAAGTTTAACTAACGACTCATATAATTTATTAATTTCTTCAGCAAACAAGATTGATTCAACTAAATTAGAAGCTTTAAAGACATTTATTAATACTAAAATTTCAGCTATAGGTAATGAATTTGATTTGAACATACACGAAATATTATTAAATGCAAATGGTGAATTAATCAATTTTGGATTTACTATTTTAATAATTGTGGTTTCATTAATAATTTGTGGAATATATAGCATTATAAGATTTGGTTTAATAGCGTTTTTTGTAATGTGAATAAACGAAATATTAGCAGTTATAAGTTCAATAGTTTGATTAATAATTTGCCATGGACAATTTAACAATGGAATTTTAGGAGCATTGATATTATCTACTGCATTTAGTGTAGTTGATTCTATCTCTAATTCTCAAACAATTAAGGAAGAATTTGGCAATAATTTAAATACAAAAAATTACATATTTGAAATTGGCCAAATAAACAATATATTTAAAAAATATGTAATAGAATCTTTGAATAAAAACATCTTCAATATATTCTTTGCAATTATTTTTGTGGCATCTACATATACGTTGATGACAGGCTTTAATGCAACAACAATATTAGTAACTTCAATGTTTATTATTTCAAATTCATTTATCAATTTATTTATATTACCAAACATTTGAAGATTGATGTATATCAAAAGATATTCAATCAAGCAAAAGAGAATTGAAACTAAATATTGAGAAACAGAAAAGATTAGCGAACAAACATTTGTTGGTATTAATGATTTTAATATCTAAAAAAGGAGGAACCAATGTTTCAAAGACTTAAAGGTACAAGAGATATATATGGGTTAGAAGAAAAGGTTTTGAATTTGATAGAAAATAAATTTTTTAAAATTATAAAAAATTACAATTTTAAAAGAATTTGCACACCGATAATTGAAGACGCTAACTTGTTTATAAGATCAGTTGGTGAAACTAGTGACATTGTTTCAAAAGAGATGTATCTGTTTAAGGATAATGGCCAAAGAGACATTGCCTTAAGACCAGAAGGAACCGCTTCAACAATAAGAGCTTTTGTAGAAAACAAAATAAATAATTTAGAACCTACTAAATTAGCCTATTTTGGACCTATGTTTAGATACGAACGTCCTCAAAAAGGTCGCTATAGACAATTTATTCAGGGAGGAGTTGAATTAATTGAAAATTCAACTGTTGAGAATAATTTTGAAATAATAAAATTAGCATATGATTTTTTAAAGTCATTAAAAATTAGTAACATTACTTTAGAAATAAACAATCTAGGCTCTTTTGAGTCAAGAAATAACTACATAGAAATATTAAAAGAATATTTTTCTAAATACAAAGAACAATTGAATGAAATTTCTTTAATTAGATTGCAAAAGAATGTTTTAAGAATCTTAGATGATAAAGAAGAAATAAAAAAAGATTTTGTAAAAAATGCCCCAAAATTAATAGATTATTTATCTTCAATTGAAAAAGAAAGATTTAACAATTTACTAGATTTACTAGACGAATTCGAAATCAAATATGTAATAAATCCTTACTTAGTTAGGGGGCTTGATTACTACAATGATGTTGTTTTTGAATTTGTTTCAAATTCAGATGCCCTAGGTTCACAATGTACCGTTCTTGGGGGTGGAAGATATGATGGTATGATTAAAAGCTTTGGAGGGCCTGATATAGGCTCTATTGGGTTTGCCTTTGGAGTAGATAGATTAATGGAAATAATAATGTCAAATATTGACAATTATCCAGAGCTAAGTTTTAATGAAACGATATTAATTGCTTATTTGAATGAAGATGAAAAAAATGAAATAAGAAAAATTGCTTATGATTTGAGAAGGGATTTTCAAGTAATTTTTCCAAGTCAAAGACAATCAATTAAGTCTCTGTTTAGACTTCAATATAAACTACAACCAAAATATTTAATTTTTAAAGAATTAAATATGAAAAAAAATGAGTTGAAAATAAAATCAGTTTTAAATGAAAGAAATATAATTTATACTAATTTAGACGAATTTAAAAATATTATTAGTGAAATGAAAAGAGATGAAAACAATGAATAAAAATTATTGTGGAACATTATGTATTAATGATTTAAACAAAGAAGTAGAGCTTTGTGGATGAATTGCAAACAAAAGAAAATTTAAGCAACAAGTTTTTATTGACTTAAGAGATTCAAGTGGCGTAGTGCAATTAATATTTCAAAATGTATCAGATCCTTTATTAACTAAAGAAAGTTGTATTTATGTAAAAGGAACTGTTCAAAAGCGTTTAGAAGTTAATCCAGCATTAAAAACCGGAGAAATCGAAGTTATAGTTTCTGATTATAAAATTTTTAATTCAGCAAAGCAAATACCTTTTGAAATTAACAATTCAAAATCAACAAATGATGCAAACGAAGATATAAGATTGGAATATCGTTTTCTTGATTTAAGAGAAGAAAGAATGCTTAATAATTTAAGATTAAGACATAAATTCTTACTTTTGGTAAGAAATTTCTTCGATAAACAAGGTTACATTGAAGTAGAAACTCCAATTTTAGGTAAATCAACTCCTGAGGGAGCGAGAGATTACTTGGTTCCTACTAGAAGAAAAGGAAGATTTTTTGCATTGCCTCAATCACCACAACTATTTAAACAATTATTGATGGCTGCCGGATTTGAAAAATATTTCCAAATTGCCAGGGTTTTCAGAGATGAAGATTTAAGAAAAGATAGGCAACCAGAATTTACTCAATTAGATATTGAAATGTCTTTTGGAAGCCAAGAAGAAATATTTAAATTATGTGAAGAAATGTGAAAGGATGTTCTTTCACAATTGGGGTACAAAATTGAAACTCCGTTTCCAAAAATGGACTTTTTCTATTCGATGGCTCATTATGGAAATGATAAACCAGATACAAGATTTGGATTTTTAATCGAAGATTATTCAAAACAATTAGCAACTAAATATAATAAAAAATTTGTAAAAGCTATTGTTTTTGACAGAAATATAAATGAACATAAACAAATAATTAATGAAACTTTTAAAAAGAATAATGGACAAATATTGGATATATTTGATTTAAAAGAAGCAAACAAAAATGAATTCCAATGTCTTTTAGATAAGGCAAAAGAAGATAATATTTCAACCCCATGAGCAATAATTTCTGCTAATGATTTAGAAGAAGATGCATTGAAATCACTTGGAGCAATTAGGACAATTGCAAATGATTTATACAAATTAGCAGATCCAAATCAATTGAATTTCTTATGATTGGTAAATTGACCTATGTTTGAATATGACAGAGAAACAAAAACATATTCACCTGCTCACCATGCGTTTACACAATTTGAAGAATCTACAATGAAATATTTAGAAACTGGAGAGCTTGAAAAAGTTAGAGCTAAATCATACGATTTAGTGTTAAACGGATTTGAATTAGGATCTGGTTCTATAAGAATTTATGATCCAAAGATTCAAAAGAAAATGTTCGACGTAATTAATCTAAGTGAAAAACAACAACAAGATCGTTTCGGATTCTTTTTAAAAGCATTTGAATATGGTCTACCTCCTCACAATGGTATTGCATTTGGAGTTGAAAGAATTTTAATGATTTTAACCAAATCAGAATCAATTAGAGATGTAATTGCATTTCCTAAAAATGCTAAAGGTATTGACTTGTTGACATCAGCTCCTAGCGATGTGACAAAAGAACAATTAGATGAATATGGATTGGAACTTAAAATTAATAAAAAATAATAATATATAAATTAAAATATATGGGTAATAGTTAAATTAATAATTTGATTTTAATTATAAACAAAACAACATAAAAAAAGAAAGGGATTTTATGCATGCTGCTTCAATAGCCTTAACAGTTATATTGGTAATAATTTCATTTGCGATAATTGGAATTTCATTTATTATGGCTCCTGATTCAAATAGTTTTAGCGGAGCTTTAGTTGGTTCAAATGACCTAGACTTGTTCAAAGTATCAAAAGAAAGAGGAATTAAAAAAGTATTAAAGTGATCAATGATTACTTTAGGTATATTACTTTTTATATTCTTGATCGCTTTAAGAGTTGTAATACAAAATGGTTAGAATTTATAATTAAAAATGACATATATAAAAAATTTTAAAAATAAGAATAACAACCCTAAAATTAATGAAGAACAAGTATTAAAATGCATAAGAGAAAGTGATGGAATTACTTTTATTGAAATTGCAAAAAAATTAAGAATTCCTGCTTTTTTGAATTTTGAATTATCAGAAATATTAAATAACTATTTATCTAAAAAATTAATTGATACGAAAAGCGATGAAAAATACATCGCTATTTATTTTCTAACTAAAGTACAAAAGGAAATATCAATAACTGCCAAAAGATTAGGATTTATTGATTTTGAAATGCAAGAAGAATCTCAAACTAAAGTATTTAGTGCCTTTCTTGCACCTTTTCAATTAAAAGCGGTATTAGACAAGGATTTATTGCTTGCAAATATTTATTATTATTATGAAAATGGCATAAAAAAATATAAAGGCGATATTATTCAAATTTTAAATCACGTAAGAAAAACTATTGTTGGGACTATTGAAAAGAGCGCAAATAAACTGTTTTTTAATGCTTATGATGAAAAAGATAGGGCTAAATTTGAAATTATTAATGAAAAAGAATGCCCAGATATAACAAAATTGAAAAATTCATTGGTATCTTGTGAGATTTTAAAACCAAATAATAAAAAAGTTGCTATTGCATATAAAAATATAATTGGTAATATTAATGATAAAGAACATGTGATTAAAAAAATCATTGCCCAAAATGATGTAAATGTCAATTTTCCACAAAATTTATTAGAATATGTTTCCTCAATACCTTCTTTTGTTTCACAAGAAGAAATTAATTCAAGAATAAATTTAATAGATTTAATGACAGTTACAATTGACGGTCTTGACACTAAAGATTTTGATGACGCCATTTCGTGTTATAAAAATGAAAAGAATAATTGAAAGCTATTTATTCATATTGCCGATGTTTCATACTATGTTAAGGAAAATGATTTAATAGATAATGAGGCTTTATTGCGTGGCACAAGCATTTACTTACCCGATAGGGTTATACCAATGTTGCCGGTTGAATTGTCTAACGGAATTTGTTCATTAAACCCAAATGAAGTCAGAGCTTGTATAACATTGGAATTAGAAATAGACAAGCAAGGGAATAATATATCATATAAAATTTATCCTAGTGTTATAAAATCAAATTATAGATTGACTTATAATGAAGTTAATGATTTTTATAGCAATATAAAAAGTGTTCCTGATGATGTTGCAAATATGCTGTTGCATGCACGAGATCTTGCAAAAATTATTAGAAGTAAAAAAATATCCGAAGGTTATGTTGACTTTGAAATTCAAGAATCAAAAGTTATAATGGATGGAACAAAGGTTGTTGATATCAAGGTAAAAGAAGAAGGCGAAAGCGAAAAATTAATTGAAGACTTCATGGTTCGGGCAAATGAAACCGTTGCTCAAATGATGACTAATTTAAAAATTCCTTCAATTTATAGAATTCACGAAAAACCTTCACCTGAAAAACTAACTTTATTGCAAGAATTATTCAATTTTTGTGGATTTAAGGATGTAAAAGTTCCTTATGATGGAGAACCAAAATCATTTGGAGAAATGATTGCAAAATTAAAAGAACATAAATTTGATGATTTTATTAAAATGGCATTATTAAGAACAATGCAAAAAGCAATATATTCATCAAATAATATCGGACATTTCGGTTTAGCTTCAAAAGCTTACTCACATTTTACTAGTCCAATTAGAAGATATCCAGACTTATTGCTACATAGATTGATAAGAACTTATATTTTTGAAAAGAAAGAAATAACACCCGATTTATCAGAAACGTTGAAACAAAAAATAAGTCAAATTGCTTTAATGAATAGTGAATCTGAAAAAAATGCAATGGTTGTTGAACGAAGTGTTGTTGATGTTAGAAAATCGGAATTCTTTGAAAATTTAATTAATAAGCAATTTTTTGCAACATTAGTATCAATAGAAAAATTTGGAATGTTTTTTAATATTGATGAATATCAAACAAGTGTTTTAGTTCGTTTTGAAAATCTTGCTATTCCAACTAATAAAGACAACGATTTTCAAGCATCATCTAAAAATAAAATCTATAAAGCAGGCAATAAGTATTTAATTACTATCACTTCGATAGATCATGAAAAAGGAAATGTAAATGCAACATTAGCTTAGCATTGACATTTTTTTTATTAATTAATTAAAAATAATTTTAATAGTTATATTTTTTTATAAAATAATAGTATGCCCAAATTGATAATTTCAAATAAATTTATAAAATCAAATTATGAAATTGATTCCACATATGAATGTGGCATTTCGTTATTAGGTTGAGAAGTCAAGAGTTTAAGGGCAAAAAATGCAAAACTTGATAATTCTTTTTGTTCAATTAGTAATAACCTAGAATTATGAATCAATAATTTAAATATTGCTCAATATATGCAGGTAAAAGGCGATTTAAAAAGAAGTAGAAAATTGCTAATGCATAAATCAGAAATATTAAAATTAAAAAACAAGCAAGAAAAATTTTCTTTTGTTATTGTTCCAGCTTCGATATATTGAAGCAATAATCATATTAAGGTAGAAATAGCTTTAGCTAAAGCTTTAAAAAAATATGATAAGCGAGCTAAAGAAAGAGAAAACGAAATAAAGAAACAAATAAAATCAAGTTATTAAGGGGGCGTACAGGCTTCGACATACATGCAAGTTATTAAGTTGCAGTAGTTTGGCAGACTATAATGCTACTAGGCTTTTTAAGCGCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAAATTCTGGTAGGCTAATGAATAGCTCGGTTCATTGTTAGCCTTTGGAGAGCTTAGCAATTAAAGTGGCGTTTTAATTGCAAAATTATGCCAATGGTTATTATGAAGATTGTTAATTTGATAATAATATAACCAAACATAAAATTAACTAAACTGTAGATACTTAATAATGAACATGTGTGGACCGGGGTTCGATTCCCCGCGCTTCCACCATATGAAAAATAAAGAGATCAGCGTTTTATGATCTCTTTTTCTTTTAATTTGGTGATATTTTTTTTAAAATAAAAATAAATAATAAAAATTATTTTTATTTTTTATAAAATTAAAATAATCTAGTTATTAAAATTAATAAATTAAATATAGTTTTATAAAACTTATTTAACTTTAATATAATTTTAACTATAAATATATAAGGAGGGGAAATGGCGGTCAGATTAGTAGTAAAGCATGAAGATGGTTGGGCAGTCAAAAATCCAAAAGGAAAAAGAGCATTGAGAGTATTTAAGACTCAAAAAGAAGCTGTTCAATATGCAAAAACTTTGCAAGATACTTCATCTGTAAATGTTCAGTCTAAAGCTGGGAAGTTTAGAAAGTTCACAAGCTAAAATGGCAATATTATTAAATGGTAAAGCAATCTCTTTAGAAGTTAAAGAAGATTTAAAAAAAGCTATTGAAAAAATGCCTCAAGAAATTAGACCTACTTTAGGAATTTTACAAGTAGGCGATTTAAGTGAGTCAAATATTTATGTAAAAAATAAACTTAAATATGCTTCTGACATAGGATTAAATACAAAACTAATAAAATTGCAACAAAATGCAAGTAAATTGCAAATTTCTGAAGCAATAGAATTATTAAATAAACAAAGCACCGGATTTATAGTTCAAATTCCAATGCAAACTAATCAAATTATAGATAGTCAAGAAATATTGGATTTAATAAGCATAGATCACGATATTGATGGTCTAACCTCTTTTAATCTTGATTTAAAAAATCCTAATAATTATTTTTTATCAGCTACTGCTAGTGGAATTTTTTTACTTTTAAACAAATACAATATTCTTTATAAAAATGCAAAAATTGGAGTTATTGGTCAAAGCAAAATTGTCGGGTACCCATTGGCAAATTTCTTAGTTTTAAAAGGATATAACGTATTTAGATACAATAAAGATACTCCTAAAAATAATATTAAAGACTTGGATATAATAATTGTTGCAACCGGCACAAAAGATCCAATCACATACGACCAAGTAAAACCTGGGGCGGTTATTATCGACGTTGGAATACACAGAGATAGCAATAATAAAATTTCAGGGGATTTAAATTTTAAAGAATTTGCTAACGTTGCCTCATTTATAACCCCTGTTCCCGGAGGGGTTGGTCCAATGACTATTTCGGCATTGCTCATTAATCTCTTAAAGGCAAGTTCTTTGCAAAATCCAATTATTGTACCATATCTTAAATTTTTAAAATCATATTTTAATTAAAAATTTTTTAGCTGATTTTGAAGGCTAAAAAATTTTTTTAAATTTGATTTATATTATTATTTTTTTGTAAAATAATATCACATAAAACAGGAGGGAAAATGATTATAGGTGTTAGTGGCATGATTGCTGCCGGTAAAAGTTCATTAGCTGAAAAACTTCATCGTTATTTTGAAGGTTCGCTTATGCTTCATGAATTTGAAGAAGACGACGAAGTATTTAATACATTTTTGAAATGACTTTATGAAGGGAAGCCTAATTTAACAATAGGATTTCAATCATATATAGTTGAAAATCATTCTACAAAATTTAATAAAATTAAAGAAGAATTTGAAAAAAGCAAAAAAGATCCAATTGAAGATATGATTTTTTTGGATAGATTTTCAATAGAACATTATATTTTTGCAAAATTGATTTTATCTAAAAAAGGTAAAAGATATTTAGATGCATATGATGCTTTGTTTGAAAAATTAATTAGTAAAGATGAATTGCCTGATTTTGCAATATTTTTAGATATTAATTTTGAAACATTTAAAAAGAGAATATTTACACGCGGAAGGCAAAGTGAAGTAGATAACTGAGATGAAAACTATGAATACTTCCGTAATTTACATGAAAACTATTTTAAAATTTTTAAAGAAATTGCTCAAAAATTTGGCTTAAAATATGTTGTAATTGACACAAATAACTTAACAGAAAAAGAAGTTTTTAACAAAGCTTTGGACATTATTGAGAGTGAAAAAGAAAAATGTCGCAAATAGACTATAGAATAAAAAAAGTTCTTTTTGATCAAGCAACATTAGAAAATCGCATTAAAGAATTAGCCAATTGAGTCAATGAAACATATAAAGATTCAAAAAATTTAATCATAGTTGGTTTATTAAAAGGCTGTTTGCCATTTTTGGCGCAGTTAATTAAAGACGTAACTGTAGATTTTGTTTTAGATTGCATGATAACTAGCTCATATGACGGAAAAGATAGATCTTCGGGAAATGTCAAAATAACATTGGATTTACAAAATAATATTGCAGATAAAGATGTATTAATTGTTGAAGATATAGTAGATAGTGCTAGAACTATGACCAAAGTTGTTTCACTGCTAAAATCAAGAAATCCTAAATCATTAAAAGTTCTTACTTTACTTGATAAACCATCTGGCAGATTAGTAGAATTTAAACAAGATAACTTTGGTTTTGAAATCGGCAAAGATGACTTTGTTGTTGGCTTTGGCTTTGATTGAGAAGAAAAAATGCGTCAATTACCATATATCGGTTTATTGACAGATAAAGAAATTAATAAACACAAAAAACACTAATAATTTTTCAATGATTCTTAAGTTACAAAATTGGCAAATAACAGAAACTTCAAATCAAGATAGTGGCACTAGCATACCTTTAGCTATTGGCTCTGCGTTTGTGGCATTTATTTTAATAATTGCTGTAGTTATTTTTATAATAAAAAAAATAATAAATAAGCAAACAAAAACAAAAGATAATTCAACTAATAAAGAATTAAATAAAGACAATAAAAATGACGAATATAATAAAAAAATTACATTGCCTTTTTTTAATTCTGAAGACCTAAAAAGAATAGATAAATTAAATTCTTTAAAAATTGAAAAACAAATCGCTTATTTATTTGACTCGCAAAAATTTTTTAAAGAAAATTTAAATAATTTATGAGTTAAAAAATATTACGAAATTATTAAAAAATTTAAATTAGAAAAATCGCCTACAAGTAAATTTAATAAATTAAAAATCAATAAATATTACAAAAATTATCTTAATGAAGTGGGCCAATGAAATTATTGCAGACACCACATTGATGAAATAAAAATAAGTGGAGCGATTTATAGTGCTCATCCTCAAACAAAAGAATATTATAAAACTGGTGATGTTATATTGGTAACATATGAACAACACTTATTTTTACATTATTTAATTGTAATGGCAAAAACTACAATGCCAAATCACGGAATGATAGTAGGACTTAAATTTCATTTTAATGATGCTATGAAATGTATTGAATATTGAGATAAAGTAATTCAAAAAATGTGTGAAAAATATTCAGTAAAATATGACAAAAATTGAAGTGAAAATTTAACTTAATTTAAATAGTGAGTATTCACTATTTTTTATTTTAAAAAAACTAATTCTCTAGATTTATTATAATTATTAATATAAAATATTAAAAAATATTAATTGCAATTTTAATTAATAAGAATTTTAATTTATTAATAATTTTTAAGGAAAAATATGAAAATAAAGCTTTTTAATAAAATAATAAATGATGAAGATAAACCTAAAAAAACTCATTTAAGTTGAACAGAAAAATTTAAACTTTCGTTGCAAAAAGAAAAAGACAATTTTAAAAATTCAATTCAAAAAACAAAAGACTTTTTTTGCAATCCTAAAAAGTTTTTTAAAGATTATAAAGAAAATGTTTTGAAACCAGAATGAAAAGGATTTGTAGAAGGTTTTAAATTTGAAAATTGGCCGATTGTTCAAGCAATAAAAAGACTAAAAAACCCTAAAAATAACATTAAAATTAAAGAAGAAAAAATTAAAATAGCAAGATGAAATTTTACTGAAGATAAAAACGAAGATCAAACAAATGATAAATAAAAATTTGCTAAAATTTTAAATTTTTTAGGTTTTAAAAAATATTTTATTACCTAAGCTTTTTATAGCTAATTACCATATTTATAAATGTTGTATAAAATATTAAAAAAAATTATTTAAAATTAATTTTTTAATAGTGATAAAATCACTATTTTTTATATTTTAAATAAAATATATTAACCTTAATTTATTATAATTATTAACATGATTGAAATTAAAAAAATATCTTATAAATATCCAGGCTCATCGAAGCTGGCGTTAGAAAATATTGATCTTAAAATTGAAGAAGGTCAATATATTGCTATTTTAGGTCATAACGGTTCGGGAAAAAGCACATTTTCTAAACTTTTATCTGCAATTTATAAACCAACATCAGGAAAAATAATAATTGATGGATTAGAAATTAATTCAGAAAATTTACTAGAAATTAGAAAAAAAATTGGAATCGTATTTCAAAATCCTGATAACCAATTTATTGGTTCAACAGTTGAAGATGATATTGCTTTTGGTCTTGAAAATAAAATGTTGAAACCTGAAGAAATGAAAACAATTATTTATAAATATGCTCAAGAGGTCGGAATGCTAGATCAGTTAGATAGAGAACCACAAAATCTATCTGGTGGTCAAAAACAACGCGTTGCAATTGCTTCAACATTAGCCCTAGATCCTAAAATAATTATTTTCGATGAAATTACTTCAATGCTAGATCCTAAAGGGCGTAATGATATTTATAAAATAATTCATAATTTACACAAAAAAACTAATAAAACATTAATTTCTATCACACATGATATGGATGAAGCACTACTAGCAGATAAATTAATAGTATTTGCTGGCGGTCATGTTATTGCTCAAGGTAAACCAATAGAAATCCTAAATAACAAAGAAATTATTGAAATTGCAAAAATAGATTCTCCATTTATCTATAAACTAAGTAGTCTCTTAAATGATATTGAACCAACATATAATAGAGAGGAATTAATTAAAGAAATATGCAAATAAAAGCTTCTGAAATTACCAAAGAATACGACAAAGGATTGCCCACTTATATAAAAGTTATTGATAATGTAAGTTTAGAAATATCAGAAGGAGAAGCAATATCTATTATTGGCCCAACTGGTTCAGGGAAAACAACTCTTATTGAACATTTTAATGGTTTAATTATTCCTACAACTGGAAAAATTATCTTTGATGAGTTACCAATAAAAATTAAAACTCCAAAACCTAAAAAACCTAACTTAAAAAAAATTAAACATGATTCAAATGAATATGAAATTGTTCTTGAAAAATGAAAAAAAGATCTAAAAGATTGAAAATTAAAGAAAAAATCCGAAAGGTTTGAGTGAGTTACAACCGATTTAGAAATTTCTAAAACCATAAGAAAAATAAAAAATATTAAAGCATTAAGAAAACAAGTGGGAGTTGTATTTCAATTTGCTGAATATCAACTTTTTGAATCAACCATTGAAAAAGATATAATTTTTGGACCTATTTCAATGGGTGTAAGTAAAGAAGAGGCTAAAAAATTAGCACTTAAATATATTGAAATGGTTGGATTGCCTATTGATTATTTGCAAAGAAGTCCATTTAATTTATCAGGCGGTCAAAAACGTAGAGTTGCATTAGCTGGTATATTAGCAATGGAACCAAAATTTCTAGTTTTAGATGAACCAACTGCCGGGCTTGATCCACAAGGTGTCGAAGAAATGCTAAACTTATTTCACAATCTCTATTTAAATGGAAAAACGATTATTATTGTAACACATGACCTTGATAATGCTCTAAAATGAACAAATAGAACAATATTTGTAAAAGATGGAAAAATAATTAGAGACGGAAATACATATGATATTTTGTCAGACGACAATTTCTTAATAGAAAATAATTTAATTCCTACTAGATTATTATCATTTGTTAATGAATTAAAAAAGAATAATGTAGATGTAGGTAAGGTAACAAGTGTTGAGGATTTAGCTGAAAAAATTAATTATTACTTAACCATGAAGCGCAAAGGAGGAACAAATGAATAAAACAATTATTGGTAAATATTTAAATTACGATACGTTTATTCACAAAATCGATCCTCGTTTCAAATTTTTAACAAATGTTTTATTAATAATATTGCTTTTTATGGCTGATCACTTTATAACTTTGGGAATTTTAATTTTCTCAATAATGATTTTTTATTGAGTAGCCACAAAATCATTAAAATCACTATTTTCTAAGTTGCGCTTGCCACTATATATTGCAATATTTCTATTGATAATAAATATGTTTACAGTTAAAGGCGCAGTCTCTTTAAATTCGGTTTTAAGTTATGTTCCGCTTGCTTCTGATGGAAATTTAGACTCCTATGTCTATTATAGAAATGAAACAATAATTAATACAATTTATTGAGCCCCATTTGGAAGCGAAAATATATTTCAATTAACCAAATTTACAATTTATAGAACGTTATCAATATTTTTAAGAATATATGGTGTTATTTTAACAACAACAATTTTAACAGTTTCAACAAAACCAGTTTTACTAACTAGAGCAATTAATGATATTTTAATCCCCTTAAAGTTAATTAAAATTCCTACAGAAATTATAACAATGATTATTTCAATATCATTACGTTTTATCCCAACATTACTTGAAGAATCTGGTCGTATCATGAAAGCACAAGCAAGTAGAGGTATCGATTTTAAAAATGGAAATTTAAAGGATAAGACTAAATCCTTTATTGTTTTGATTGTTCCACTATTTGTTTCTTCATTTTCAAAGGCGAATGATTTGGCTGACGCAATGACATCAAGAGGTTATGAACCTTATGCTAAAAGATCTCACTATAGAGTGTTAACGCCTTGTTGAAGAGATATAATTGCTGTATTAGTCATAGCTAGTTTAACAACATTAGTTTTAGTGGCTCAACTTGACGCTGTTCCATTGCCAAAATGATGATTAGTAACATTTCAAAGAGTTTAATATGAATAGCAATAACGATATAGAAAAAATAAGAAAAGAAATTTTTGATTTGCAAGCCAAAATTAGTGCTTGAGATAATGCATATTATAATTTAGATTCGCCTGAAGTTCCAGATGAAATTTATGATGTTGAATTTAATAAATTAAAGGCGCTTGAAGAAAAATATGCCTCGTTTTTTAGTAATGAAGAATTAAAAAATTCTCCAACAAAAAAAATTAATGCATTGGCTTCCGATCTATTTAAAAAAGTAAAACATAAACGTCCGATGCTTTCATTAAATAAAGCATATTCGTTAGATGAAATTCAAAAATTTATTGATAATATAAAGAAAATTACGTCTGATTTTTCTTTTTTTATTGAGCCTAAAATTGATGGCCTTTCAATTTCTATTATTTACGAAGATGGCAAATTAGTTAGAGCAATAACAAGAGGAGATGGAATAACTGGCGAAGATGTTACGGCAAATGTTGTTCAAATGTCTTGCATCCCTAAAACTATTGAATATAAAGAATCATTAGAGGTAAGGGGGGAAGTTTATTTAGCAATATCTGATTTTGAAAAATTAAATTTAAAATTAGGGCAAGAAAATAAAAATAAACTTGCAAATCCTAGAAATGCTGCAGCAGGCACATTAAGACAATTAAATTCTCAAATTATTAAAGAACGAAATTTGTCAGCATTTTTGTATTATGTTGTAGATCCTTTGCAACATAATATTAAAACAATGCAAGAAGAACATGAATTCTTAAAAACTTATGGGTTTCCCGTTACTCCCGAAGTTCAACATGCTAATACATTGCAAGATATTGAAGAATATATTAGAAACTTCAAGAATCAGAAATTATTAATAGATTATGAAACAGATGGAGTTGTTATAAAGTTAAACGAGGTTCAATATTTTGACAAGTTAGGATCAACAGCTAAGTTCCCCCATTATGCAATTGCATTTAAATATGAACCTAATGTTGAAACAACAACAATAAAAAGAATATATGCGACAGTAGGAAGAACTGGATTGGTTACATACAATGCAGAATTAGAACCAGTAGAAATTTCGGGTAGTCTTGTAAGCTTTGCAACGTTGAATAATTTCCAATACATAAAAGATTTGAATTTAAATGAAAATGATTTAGTATATATTAAAAAAGCTGGAGAGATTATTCCTTGTGTAATTGGCTTAATTTCTGGCAAAAAAGAAATTGATCATTTTCAAAAAGTAGAATTCTGCCCATATTGTCACAATAAACTTGTCGATTCAGAAACGGGATTGGAGCAATTTTGTTCAAATGATCAATGCCCCGAAATACAAAGAAGAAAATTGATTCATTTTTGTTCAAAGGATGGTTTAGATATTAATACTATGGGTGAGAGAAATATTGATCTATTTTTAAAAAATTCTCTAATTTCTAACCCTATTGATATTTTTAATTTAAAGAACAAAAAACAAGATTTATTAAAATTAAATAGAATGGGTGAAAAGTCTATTGAGAATATCTTAATTTCTATTGATGAATGTAAGAATAAACCTTTATATAAAATGATTTCTGCTCTTTCAATCCCACTAATTGGCGTAAAATTAGCTAAATTCGTAGCCTCAAAAATTAAATCATTACATAATTTATTAAATTATGATTTTTCACAATTAATTGATTATCATGAAATAGGAGATAAAATTATAAATTCTATAATTTCTTGAATATCTAATGATTCTAATAAAAAGTTAGTTGAGGACATTATTAATCTTGGATATGATTTAAAAGATGAAAATGAATCAATTACTAATTTATTGGAAAATAAATCATTTGTAATAACAGGAACATTGGGACACCCTAGGTCATATTTTGAAAGCTTAATTGAAAAAAATGGTGGTTTAATTCACTCAAGTATTTCATCTAAAACTAATTATTTATTAGCAGGTGAAAACGCCGGATCTAAATTGCAAAAGGCAAATAAATTAAATATTACTATAATAACTGAAGAAGATTTATTTAAAATGATTGGTCAATAAATAATATTCTCTCTTTATTTTAATATATGTATATATTGAAATAATATAAAAATTAATAGAATATCCTAATATTGAATTTTTTTCTACTTCAATAATCAAACAACCATAGCTTAAAGTATTTATTACTATTTCAAATATTGAAACATACATTTTTATTATAGGTTTAACTATAAAAGCAAATAAACAAAATACATAGTTAAATATTGAAATTGGTAGAAAGAATAATTGATAAAATATTCCTAAAATGTTAAATTTTGGATTAAATGAAATTACTATTGAAATAGAAGAAATATAAGGTAATATAATAAATAGAAAATTTACAAATATATTGTTTTTGTAAATTTTTTTAATGTCTAAAATCAAAAAAATAATTAATGTTATTGTAAAAGATAAAATAAAAGAATAAGAATACATGGTTCTAATATCAAAGAATATCAATAATAGTGCTGCAAAACTCAATATGTCAATGTTATTAAATTTGCCTTTAAAAATGTTTTTGCTTGCAATTTTTAATATCAAAAACAAAAACGCCCGACTTGCAGATATTGAAAAATTTAATAATTTTAGGTAAAAAAATAACATTAATATTGAAATCAAATCAACAATCAATTCCTTTGAATTCAATCATTTTAAAATTTTTGATATAAGCAAATAAATTATTCCAAAATGAAATCCGCTTATTGTAAAATAGTGCATAACACTTAGTTGTTTTAATTGATCTACAAACAAATCATTAGCTTGTTGAATGTTGAATAAAACAAGTGCTAGAAATTTTTTTGTTTTATAATCATAATCTTGCAATATTGGAATTTTTTCTTGATATGCATATGCTTTTGAAACCAATTTAATATTTAACAATTCAAAATCAATTTTATTGCTCAAACTAAACATATTTTGATTTTTAATTATTGATAAAGTGCCTGACAATTCGATATTATATTTGTAAAAATTATTGAATTCTCGACTGCTTCAATTTTTAACTAAGACATTATAATATTGATATTTAATTAATATTGATTTATTAAAAAATCTCTTTATTTCAAACTTACCACAAATATTCTTTCCATTTATTTTAAATAAATCCAAATATCACAAATAATGAACTAAAAATATTAAAGGAAATACTATTGCAATTTTTATTCAATATTTTAAATTAAAAAAATTCACTGCAATTATTAATGACAACATTATTGATGATATAACAATATTAATTTTATAAATAATTAAACAATATAAGCAAAAGCTCAATGCTACAAATAAATTTGTATTTAAATTATTAAATGATTTTTTAATATAGTTAAAGTTTTTTCACCTACTCCTGGTAAATTTTCCAATTCCTGTCATGTTATTAAATAATCCTTAGCAATGAAGTAGCTTAAAATAATTTTTGCTATATCTTTTTTTATTCCTAAATCCTCTAATATGCCAACGTTATTGACTTCTCTAATAGAAATTGAATTATCTTTTTTAAAAGGAATATTAATTGATTGATTTTTTTCTAATTTTAAATTTAAATCAATATTGTTTAAATCTGCACCCGTTTTAAGACGTGCTTGTTTTATTATTTCCAATAATTTAGTGCCTTTTTTAATTTCATATGAGTCAGGATACTCTATTGCGCCTGATATTCCTATTGTTATTATTTCATTTTTGTTTTTGATAAATAATTTATTGTTATTTTTTATATTTGAAATGTATTTATTAATTCCTATTGACATTAGTATTATTATTGATGCTGATGAAAATAATCCTAGTCCCAAAAAAATTTTTCGTTTATTCATTCATCCCTCCACCTTTATCTTAAATTAAAGAATTTTATTATTGATGAAAATGGGAAAAATTAGCGAAAAAACCGTTTTTGATTTTATATAAAAAAATAAAAAACGCAAAGCGTTTTTTATTATTCTCAGATTGCGTTGTGTCAAACTTCTTGGACGTCTTCATCGTCTTCTAAAAAGTCAACAAATTGTTCAATTTGGCTTGCTTTTTCTTCATCTACTTCAATTTCGGTATTTGGGATATATGTAACTTCAGCTGTTGTATAATTTTCAATTTTGAATGTTTCATCTAATGCTTGTTTTACTTTTTGAAATGCTGAAGGGCTTGTAATTATTGTATAAGTTCCATCTTCATTTCTAAAATCTTCTGCTCCCGCATCTAAAACTGTCATCATGGTTTCTTCTTCACCAGAAACATTACTTGGAAATTCAATTAAACCTTTTTGATCAAAAACATATGGTATTATTCCTTGTTTTCCTAATTGACCATTGTGTTTTGAAAAGAAATGTTTGATATTCGAGCTTAAACGATTGAAATTATCTGTTAAACAACTTACAATAAAATTTACACCGCCAAATGCTGTACCTGAATATAAATAAGATTGATAGTTTGCAGCTTCTTTAGATCCGCCAGCTACTTTTGCAATTGCCTTTTCAATGTTTGCTTTAGGCATACTTTTTGCCTTAGCCTTAGCAATTGCTAATTTTAGCGATGGGTTAGAGTCTGGATCTGGTCCGCCTAATGTTGCAGCAACAATAATTTCTTTTGAAAATTTTTGAAACATTTGGCTTCTTTTAGCATCGACTGCAGCCTTGTGATTTTTTGTGGTAGCTCACTTTGAGTGTCCTGACATATTTTCTCCTAAATTTGATAGATAAATATAGGATATTATACCAAAAATAACACTCTTATTTTAGTTATTTTATATAATAAGAATATGTATTTAATAAGAGGAGAAGATAGTTATTTATTAAATCAAGAATTACAAAAAATTATTGATTTAAAATTTGAAAATCAAGACGATTATTCAGTAATCACTTACAATGAATCTATTGATTTAGAACAACTATCAGATGATATATTTAACCAATCATTATTTTCTTTTAAAAAACAAGTCATAGTTTTAAAAAACATTGATTTTTTTAATTCTAAGAAAAAAAATGACAATAAAAAAATGAACGATTTTTTACAATTGCTTGAAAAAGGTCAAGATAACAATTTAATATTTATTTTGCAGGATATTAAAAAAAATGATATTTCTTTTAATCCTTCATTGGCTTATAAAAAAATTGAATATATTTCAAAAATAATTGAAATTAAAAAACCAAGTGATAAGGATGTATATAACTATATAAAGGAATATATTTTAAAAAATGGAGGCAACATATCTTCAATTACATTACTTGATTTTTTGTCAATGTTTCCTAATGATTTACAACAAATCATTAATGAAGTAGATAAATTGCTATTGCAAAACAAAAATATTGATATGTTGTTGATCAATAATTCAAATATTATTTCTTCTGACAACATTGAATTTAATTTATCAAATTGTATTTTAAAACAAAAAAGCATTGATATTATAGAAGCAGTAGATGAACAAAAGAAATATGGTAATGGTGCTAGTGAAATTCTAAGCCAAATAAGTTATTTGTTTTATCAAATTTATTGCACTTATTTGTTGATGGAAAATACCAGTGATAATGAATATATATCAAAAACTTTATCAATACATTCATATAGAATCAAGTTGTTTATGTCGTATATAAAAAAAACAAACAAGTATAAATTAAAAGAATTAATAGTGTTACTTGCTGATATAGATATAGCAATTAAATCAGGATTATTAGATGAAGAAAATGCCTTAAATAAATTTATTTTATTTTACATAGAAATATAAAGTTGGTGTTATGAAAAAAATTTCTATTTATGAACTTAATTTAAGCACTTTTAATAATAAAAAGGGTTTAAACAAGTATTTGTCAATTATTGAAAAAATTGAATATTTAAAATCATTAAATATTGATATCTTAGCAATTGATGATATATTATTGAAATGCTATGAAATCAATGGAAATTATGAATTTAATGATTGATCAAATTTGCAAGATTTTAAAAAATTAGTTGAACTTTGTTCAGCCAATAACATTAAAATAATGCCAACAATCAATATTGCAAAATTAAAAAAAAGTATTATTGCAAAAAAGCATTTAATTTCTATTTATCGAAACAAAAGCAGTGAAAATGAATCAAATAAAAATGATATTGAATCTTATTTATTAACCCAAGAATTTCTAGTTCCCACCATTGAAAATATTTCAAATTTAAGCGTTTTCTTAAAAGAAATTATTAGTTTTTATAGTGTATTAGGTATTAAGGGATTTATTCTTGAGGATTTTGAATTTTTAATTGACAAAAAAACAAATAACATAAAGTCAAAATTTAGTTTTTTATTAGATATATATAGAATTATCAAACACATAAATTTTAGTTCTATGGTTATTTGAAAAACAAAGCAATATAATGATTTTTTAAACATAATTGATATTGCTAATCAAAATGATTTAATATTTTTTGATTATATTTATTTAACTCATTTATCTGACATAAATTTAAAAAATTCATGAGATTGAATCAAATTCAATAAACTAAATGTAAAAAAATTATTTGAATTGTGAAAACCTTTTTGCAATAATGAGCAATATATACTTTCTTTATCATCAAATTATAGCGGCTGGGTTGTAAACAAATACGGAGACAATTTATCATTTTATAAAGAGAGTGCAAAATCTCTATTAATGTTTTTAATGCTTGCAAAAAATTCTTATAGTTTATACAATGGCGATGAATTAGGAATTCTTGGTACTGATTCAAAGAATATTTTTAATTTTAATAACATTAATTTTAACGAAGAAAAAAGATTTTATCAATCCAAAAATATTTCTAGTCAAAAATATATTGAGTCTCAAATTAAATTCAATCCAATAACACTACAAATAGAATTTCCATGGACTAATAACATTCAAAATTTAGATTTTATACAGCCGAAATTGCGTAACAAAATCAATGTTGAACAACAGTTAACCAGTAAAGATAGCACGTTTAATTTTTATTCAAATTTAGTTAGAATTTTAAACAAATCAAAATATTCAATTTATTTTAAGGGTTTTAACAAAGTTTTACTTTGCAATAATTTAATAAGTATTGAATATAGTACTAGTGAAAATAAAAAACTTTTAATTTTATTAAACTTGAATTCATTTCCAATAAAATCATATATATGAAATCAATATCTAATTCTTTCAAGTTCTTATACAAATAAATATTATTCAAGTATTCCGGAATATTTAAGCCCATATGAATGTTTAATTTTAATTAAAGATAATAATTAATTAGTTTATAATTTTTAATATATTTTATTTATTTAATAATAAAGGAGTCTTATGCAACTACAATTCGATGCATCACAAGTAGCAAAGGAACAAAAGGCGTCAACAGTTGGTTCTGCAATTGTTTATACACTTTTAGCTATAGTTTTGGCAGCCGTCTTAGCTTTTTTAATTTGAAAATTAATTAAAAATAAAGTTTTAAAGAAGAGGGCTCAAAAAGTGGAAATGCAAAAACAAAAAGAAACGTTAGCACTATTTTATCAATATATTTTAAGCTTTTATGAAGTTATAAAATTTACAAACCAAGAACTTAAAAATTTTGAAGTTTCAATAAGCACTCATCCAATGGGTGAAATTAAAGAAGGCGCTAAAAGGTTACTATATCGTTTAATTACAAGAGATGATTTTTCATATTCATTTGTTAAAAATGAACAATACAAAACCTTTGTAAAACACGCCGAATTAATAAATATTACAAATTGCAACTTATGAGATAAAAAAATACCTGAAACAATCAATTATTTTAAAGAACAATATGAATTAGTCCCATTAGGAAAAACAAGAGATGACTATATACAATTAGTTCAAAAATCAATATTAGAAAGATTTTATAATGAAAAATAAAAGTCAAAAGAAACAAAAGATAATAGTCTCTAAATTTGGTGTTGCAAATTGATTAACAGTTTTAAGAATGCTACTAATGATTCCTTTTATTGTTTTTCTTTCAACTTCATTTGGTTTAATTTTAAATGGTGTTCAACTTTCTTACAAAACCAATAAAGGAATAGTGCTATCCATTTTATATTGAATAAATGTTGCAATTTTTATCATTGCAATGATAACTGATTTCTTAGATGGATTTTATGCAAGGAAAACAAAAACCGTATCCTCATTTGGAAAGATATTTGATCCAATTGCTGATAAAGTTGCAACTACATTGATGTTACTATTCTTAGCGACATATAATTTTGTTTATATACCTATTGCAATTTTGTTTATTATAAGAGACATAATTGTTGATGGTACTAGAGTATATGCTGTAAAGAAAAACATTGAAGTATCAGCAAATTGATTTGGTAAAATTAAAACCATTTTAGTTTCATTAGCGTTAGTAGTGATTTCAATAGGAGTTCCTTTTATATCAGATAAATCAGATTATAAATATATACTATTTTATTTCAATATACCTTTAATAATAGGACTATTATTAGCGTGAATTAGTGGCATTATCTATATTATTAAATATACAAAAGGAATTAATAAAGAATTGCTTCAATCATATACAAAAAATGATTCAAATGCAACAAATAAAAATAATCAAGATCAAAATAATAATAATAAATCAAATCAAGATTCTAGCCCATCGCCTAATTGCTAATTTCAAATAAAATTTAAAATGTAACTCAATTGTTACATTTTTTATATTCTTTTCAATAAAATCTCTCAATTATTATTGATTAAATTTATAATTTAATCAAAATACGTTTAAAAAATTTATAATTCTAAATATATTTTAAATGTGAGGTACTTGCAATGAAATCAAAGAAAATAAACTTATTAAAATTATTATTAACATCGTCAGTTGCAATTTCGTGTGCAATATTACCTGTTTCTTGTTCTTTATTATCTAATGATAATGATAAAAAAATAACAATAAAAAATTATGAATTTAATAATTTATCAAGTGAATACACAATTGAATCTAAAAAATTAAATTTATCATTTGTTAATGATGGCAATGTTCCATATGTTAATTTGCTACAAATGATAAATTCTTTAGATGGATTTTTTGATAGAAATTATATTAATTTTTCGACAAATATTCTATTTTCTGAATTAAAAATGACAACTAAATTTGGATATATTTCGTTTAACTGAAAAACAAATAAAATTACTATGCCAAACACTTATTATGAATATATAACAAAAAGCCCAGGAAAGACTAATTTCAACAAATATTTGAAATCCAAATCATATTATTCAAAGGATTTTAATAAAAATACAATTGTTATAGATTTAAAAAAATATAATTTTGACATTTTATATCATAACGGTAATGTTTTGCTACCATTAGCAATATTTAATTTGTTATTTTGCTCAACTAATTATTACAATTTGTATTTCAATGGTAACAAAATCTATGGGTGCGATTTTGACATCAAATCTAATGTGCAAGAAATTCCTCAAATTAGAAATAGCAAATTTCAAAACTCACTTATTCCCAAGGATATAGTTGAAGCAAAATTTAACTTCACTGCTTTACTAATTGATTATTTTTACGGCCTTAAAGAATATAATAATATTAATAGTTTTTATTCTACAATTTCAAATGAACAAAAGCAAAGAATGCTTAGTCAAAATCCAGTTATTTCAACAAAGGAATATATAAAATATTTATACAATTTAAATGATTTACACACCTGAATTTCAATGCCTTCATTTTATAATCACAAGGATTCTAGCATTGCAAAAGATTTCTATGAATATACCACAAATAAAAGAGAAAGAATTTATCAATCTACAATAAAATATTTAAAAAATAATAAATTAAATAATTGAGTAATTCAAACAAGCAAAGAAAACAGAGAATTATTTGAAAAATACGGTGTTAGATTCATTTCAAACAATACCGCAGTAATTTCGTTTAACTCATTTAAAACCTTTCAAAATTTAAATGATAATTCTACTAATAAAGGATTTAGCGAAGACACATATTTAGGGTTTGCTAATGCTATGAAATTAATCAAGAAAAACCTAAGAAATATGAAAATAAAAAATATTATTGTAGATTTATCAACTAATAGTGGCGGCAATATAGGCGCCATGCAAAGGGGATTGGGTTTTATTACAGATGAACCAATATTAAACAAAACATATAATCGTTTAGATGAAGAAATAAATATTAGATCTGTTGAAGTCGATACAGATGGCGATGGAGATTACAAAAACGATGCTTATTCTGAATACAATTGATATCTTTTAACCGGGGTAAACACTTTTAGCGCCGCAAATACCTTTAGTTCAATTTTTAAAGAAATGAAATTGGGCAAGATAATAGGACAAAAAACAGGCGGTGGTGCGGCATCAATTTTACCTGTTGTTTTGCCAGATGGAACTTCAATAGTAATAAGTTCTAATGATTTATCCGTAACTATGAAAAATAATACAATTAAATCTATTGAAGGCGGAATAGAACCCGATATTTATTTAGAATACAAAGATTTTTATAATGACCAAAAACTATTAGAAATAATAAAAAATAAATAAATCAATACTTATCATCATTTTTATAAGGATTAAATTCATATTGTTCAATGTTTAAAAATATTGCAACAAAAACAAGATAAGAGTTTCTAAGCAATTATCTTGTTTTTTTGTATAAGAATATATTACAAAAAAATAACTAAAATAGAAATGTATATTTCACAGTAAATAAATTTTTCAAATTAATCAACCTTGTTGAATTTTTAAAACATCAAACCTACCTAAAACATTAGGTCAGTCAATTACCAAACCATAGTTTTGTTTAATTTTTTCTAATTTCTTAAAAAATTAAAAATAGAATTTTAACAGGCTATTGCAATTTAAAAATGCTTTTTTAGCTACAACGCATTTTTAGTAATGTAAAAATGAATTACATCAATATAAATAATTAGATAATAATTAAAATAAAAAACCTTTCATAGTAATTTTATCTACTAAGAAAGGTTTTTTAATTACTAATTTTTAATAATTAATGTTAGCAAAAATTTATTTAGCACTTTTTTTAGCATCATTTATTGCTCCAAAAACTTTTGGCAGTTTAGACATTAAAACATTTTTTAAATCTTTACTATAAATATTTGTGATTAGTTGCTTAAATATCAATATTATTCCCTTAAATTCATTAAAAATAATTTCATATTCTTGTTTTGTTATTTTATTTGAATTTACCATATCATTTAATTTCTTTTTTAAATCATTGCCGGATGGATCAGAATATTTTGGCTCTATAAATTTTTCTACATCAAATAATATGGGCCATCCACCCCAAATTAGCAATACCTTCTTTCCTGCACCTTTATTATCATATTGTGTTTGTTTTGGATCTTTACCTTCGAATAAATAATCAAATTTAAGATTGCAAAAGTAATCTAAAATATATTCTAAATGTTTTATTAACATGTTTTTTAATTCATTATATTTGCTTTCATTTGTTTTTAAAAATTCATGTTTTTCTTTAAAGCTATTCAATATTGGAGTTAAATATTCAATGAAAACATTAGTTCCATTTTTAAATTCTTTAAACATTTTTATAGCTCAGGCTCCAACTGTTTTTGATAAAACTCAGTCTGCTGTTAACTCTCTTAAACCAGTAGAAACTTTATCATTAATGCCTAATTCTAATAATACATTTAACATTTCATCATTGGTGTTAATAATTAAATTAATTAAGATGTTGTAAAGTTTTTTAATAGATTCTTTGTCGGAATTAAATTGACCAAATACATTAATTGCTTTATTTGCTAGTTCAAATAACGTTTTTTCTATTTCTTCGTTAAACTTTTTATATTGTTCAATTATTGCTTTAATTACGTTTTGATGAGCGGCTTGTTTTTGTTGTTTTTTTTTGCTTCATTAATCTTTTTTTCAATATTTGTTTCAATATTTAATTTAACATTTTCTAGATATTTTTTAAATTCATCTGATTTTTTTACATCTGATTCTAAATTTTTAGTTATTTCACTTATATCCTTAGCAAATCCATTAAATTCAAATGTTCTTGATTTAGTAGACTCTATTGGATTTTTGCTATATTCTTTCAATAAACTTTTCTTCGTTTTTAACTTAATTTTATAAGTTAATTTTCCTAATGTTTTATCATATTCAACTGCCTCTATTTCATAGATATCTGCAAAATCACTATCTTTTAGATTAAATTTATAATCATTAATTGTAATATCTTTAACATCAATAGATTGCTTTTCAAAATCATTATTTTTAGTGAATTCCATTGTAAGTTTATCTAATAAATCGTCAAGCCTTTTTGATTCATTTTTTAATTCTTCTTTTATTTTTTCAATGCTTGCTTTGTCATTGAAACCTGATTTAGTAAAGGTTTTTATTTCACTATAATCGTCTAATTTGAATGTTATTTGTATATTTATATCATTTGCATTTTCACTTGCCAGTAGTTTATAATCTTTTTGAATTTTAAAATCATCAATAATATAAGATTCTTCTCCGCTACCAAAATTAATTTTTTCTCTAAAAAAGAAATTGTAGCCTTTTTCTATTGATTCATCTATTGTCATTGATTTTGCTAATTCTTTTTTGGTGTAATCTTTAAAATCAAAATCAAAGTTATTTGCAAAATTTTTTAAATTTCGTTTATTCATAATATTAGCAATAGAAATTTCACCCGAGAATTTTTTAAAAAGTTTATCATTTGCTGATTTTATCTTTAAAATAAATTCATATTTTACAGTAGTAGTATTTACAAATTTATTTAATGACACAATAGCGTTTTCATTTTCTTGATTCAATTCAATAATTGAGTTTTGATTTAGAAAATCAGAAATACCTAATAACTTAGCAATTTCTTGTTTATTGTTAGCAATTTTATTTTTAAAATCTGCTTTAGTATAAACACCATCAGTTACATAAGTTGATTCTTTTGAAATGCTTAAGTTATTAATTAAATTAAATGTGTAATTTTCATCACTTAAGAAATTGAATGTAACTGTCTTTTTGTTTGATTCTATTGTTCCATGTTTAAATGTTTTATTCAATAACGAAAAGGCTATTTCTAATTTTCCATTGGCATTGGCATTGATTTCTGAAATATTTAATTCCAACCCTGCTAGTTCATTACTTTTTTGCTTGTTTAATTCAAAGTCATTTTTTGAAATCATTGAAGATTTTTTAGATGTTAAATATTCATTATTTTTTAAAGATGTAATGTTTTCTTGTTCAACTAATGTATCCAATCTTTTTTGTTCAATATTGATTTCATTTTGCTTTTTAGTTAAGAAATTCGTAATTTTTTTTGAAATTCTCGATTCAGAAACAATAAAGGTTTTAAATTTTTCTTCTTTAACTTTTATTGTTCAGCTTACTGACAAAATTCCTTGATCATCATCTGCAATAATTGATGGATTTCCTTCAATAGTATATTTTGAATCGCTTATATTTAGATGAATTTCTTTACCTTCAACATTTGAAGGTAATATATTTGACTTATCATTCTCTTTATCAAAAACTAAATTTGCTTCTATCTTATCATCGCTAATTAATTTATTCAACTCTTGCTTGCAAGCCTTTAAGTATTGTTCTTTTGTATAAAAACCTGACAATGTTATAGTTTGTTCATTTGATTTTAAATCTTTATATTGCTTTTCTTTTGTTTTAACAACAAATTTAATTTTTAAGCTTCCTTTATCATCATTTGATTCACTTATTTCTAAAATTTCAATATAAAAATCATTGCTAATTTCTGTTTCAAAATCGTTTATTTGAACATCAGAAGCAAATGTTTTGGCAATATTTTTTGAAGAAATTAATCTTAACGTTGTTGTTTCTTTGGCAACTTTATTTAAAGATTCTATTTTTTCGTTTTTATCCATTGTCTTAGTTTTATAGCCACTTAATTCAACTTCAATGAATTTGCTTGATTCGCAATATTCATTAATTTGTTTGTTCAATTGTTTGATTGCTAATCTAATACTTAATTTTCCACTTTCATCGTTTGGAATTAATTGCTTTACAACAACTTCAATATTTTTAAAATTATTATTTGGCTTTGGAATTTCAATATTTAAATCTGATTGTTTGATTGAAGATGCAAATCTTTTAATAGCAGTTTCAGGTTTCTTATCGCTTACTTTTATATTTAAATTTCCTATTAATTCATTTAAATAAGCAATTGTTTCATTTCTTTGGTTATTAATAAATTGTGCATTTGTTAAAAAATCTGTCAATTTTATTGTTTTAATTTCTGTTTTTAAATCGCTGAATTTTGGATTAGTACTTTTTAAATAAAAACTAATTAACAATGTTCCTTCTTCGTCATTGGCTTGCATTTTTTCAATAACTAACATTGCTCCATCAATATTTTTTTATTGAAATCAACATTTTTGATTTCCGAAGGTTTTTTGTTGTGCTTTTCAAGTGCCAAAAATTCGTTTAGATCAATTGAATTAACTAAATTAGAAAGTTTCTTTGATGTTTCTTGTCTTTCTATGTTGAGTAATGTATTAAATCCCACAAATTTTTTAGTAACAATATTTTATTTTAGATTCTTTTTATTGTCATTGTATCAATAGCTAATAGTTAATTCACCTTTTTGATCGTCATAACTAAATTTTTTTTGCAATTCATATTCATTATTTAGATTATCGAATTTGAAATTGCTTAAATTTTGAATATATTGAGATGGCAATATTGTATTTATATTTTTTTCATTTATTGATAAATTAACTTTATCAACCGCGATATTAAGATTTTTTAAAAATGATTTAAAGCCCGTAATTTTGGTTGTATAAACCTTTGATTTAATTCAATATGAAGCGCCAGTTTTCTTATCAATATATTGTTTTTCAAAATAATAATTTAATTTAACTTCCCCATTTATATCATCAAAAATTAATTCACCATTGCTTCTTAAATAAGCTGAATTTAGTTTTTTAGATGTTTCAAAATCATTTCAATTCAATGTTGAAGCATATGTTTCTGATTTATTAATTTGTTTCTTTAATTTTAAAGATATCTCATTTCCTAACTTATTTAAAGTGTTAGCTTCTTTTTGCGTATTAGCTATATTATTCAATGAATTAAAACCATAAAATGTTTTTGAAATAATTTTTGATTTTATATCTTTGTCTTTTTGTACATAATAATATTTAACTTCTATTTTGCCTTGTTCATCGTTTGCAATATATTCAGTTTTTAATTCATATTGGGAATCGATGTTTTTAAATGAAAAATTGCTTTGCAATTTAATATATTGCGAAGGCAAAAAGTCTTTTTTATTAAATTCATTTATTTCTAGATTAACTTGTTCAATTGACGAATTTAAAGGAGCAACCAACGCTTTAAATCCTGTATATTTTTGAGAAAACGTATTTGATTTTATTAACAGTGGTTGTCTTGTTTTAGAATCGATTATTGATTTTGTAAAATAATAATTTATAATAACATTCCCGTTTACATCATCTGCAATAATATTAGAGATTTCAAACGATATTTCTTTATCAAGATTATTGACATAAAAATTTGATGAATTAATTTCACTTGGAAGTATCTTATCCTGTTTAATAGAAAGTTTTAAATTTGGTTTAATATTTCTTGTTTGTTCCTTTAATTTTGTTGTTTCAGTTTCTTTATCTTGCGTCTTGTTGCAGGCTGATGAAATGACTCCTGATACAAGCAAAATGGCAGTAGCGCTTAAAATGGAAATGCTTTTGATTTTTGTATTCTTTTTCATATCTATTTTTAATATTTCTTTCTTTTTTAATTAGATAAAAAAAGCATAAATGGAAGACTCCCATTTATGCTAAAAGTAAAAATTATTTTAAGATTTCTAACACTTTTGGTAAATCTCTCAATGATTGTTCAAATAAGTTAATTGAGTTTTTTAGGCCTTCATTTAAAACGTTGTAAATTGGTCTAATTACATCTTTTACTTTTTTAATATCTTCATCAGACATTTTTACAGATTTTAATACTGCAACTGCTTCTGCCTTAGCATCAGATAATAATTTAATTAATTTTTCAAATGTTTTTGAATATCCTTCTTCAGTCAATTTACTAAAATCAAATTCTTTTAATTTCTTAGCGGTTGTTTCAAATAAATTATCTTTTCCAGCAAATTTATCATTAACAGCTGTAATTAAATCGTTCAATTGAGCTATTGCTTTTGTTGATTCGTTTGTTTTTAGTGTTTCAAAAATTGCTTTTGCATATTTAGAAATATTTTTTCCAAATGTAACCAAGAAATCTGCCAACCCATTTTCTTTTTTAATTAAATTAGAAATTTTTTCTTTTGTTAAATATTTTTTAATTGTCATTAATCCATCAGAAACTTTTGTTTTATTTGTGTTGTATTCTTTTAGTGTTGACAAAATAATATCATCTAAAATTTCATTTAATTTAGAAATTGCTTCATTATATGTTTTTGTAATATTTGGCAATTCTTTTTCAGAAACTGCAGCTATAATTTCTTTATTTTCAATAATTGGCTTTAAAATGGCTTCTGATTTAGTTTTTAATTCTTCTTGATATTTCTTTAATTCACTTTCAAAATTTGCTTTAACTGTAGCATCTGCAATACCTTCAGTTGCTTTTTGAAGTTCGCTTTTTTCTTCTGGTTTAACTTCTGGTGTTGTTCCTGGTTTTTCACCTTCAGGCTTCTTTTCGGGTGTTGTTCCTGGTTTTTCACCTTCAGGATTCTTTGGATCTTCTGGTTTAACTTCTGGTTTAGTTTTTACGCAACCCGCAGCAACCAATGGAGTTGAGGCTAATAATGGAAGTGCTACCCCCATAGTTAATAAAATTTTTTTGGATTTTTTCATTTTATAATTTCCTTTCTATATAAAAATAAAATATATTAACTTCTTATAATTTACCATATGGATTGTTTAAGAACAATTTAATAAATTGGATTTCTAAAAAAAAAAAAAAAATGAACCAAAAAGTAATTTTTTACTGGTTAATTATTAAATTACTTTTTAATTTAAGATACTTTTTATAAGCAGGAAAAAATTTTATTATTTTTAAATTTAATGAATAGCTATGCTTTTAAGATTTAAAAAAAATTAAATATTAAAAAATGTCTATATAAAAATTAATTTTTTTTAATTTAACTTATAATTTATATTAATTATTTATATATAAATATTTTAAAACACTATATTTTATAGGAGGACACTATGCCAAGAGAAGGTTTAACCTTAAGATGTGAAAAATGTAAAATGGAAAATTACATTACAAAAAAGAACAAAAAATTACATCCAGACAAAATGGAAGTACAAAAATACTGCTCAAAATGTAATGCACATACAACACACAAAGAAAAGAAATAAATTTCTTTATTAGCATTAAAGCTATTAATATAAATAAGGATTTAACATGATTAGAGAAAAATTAAACAAAATTTTAAAAGATAACGGGATTGATGCGATTATTTCAGAAGCTCCTCAAACTCGTTTGTGATATGCCGGAGTTCAAACAAGCGATGGATTTCTTGTTATTGAAAAGGAAAAGGCATATTTATTTGTTGATGGAAGATATATTGAATATGTAACCAAGAATTCAAAAAATGTAGAAGTTGTATTATTAAAAGGCACTTCATTTAAAGATTTTTTGGCTAAAAAGAAATATGCAACTGTTGGTGTTGAAAAGGACTATTTAATACTCGAAACATTTGAAAATTTAAAATCAATGTTGCCAAAATCTAAATTTGTGTTAGTAAATGGACAAGAATTTAGAATACTAAAAGATGAAGAAGAATATAGCAATGTTCAAGAAGCATGTAACATTTCTCTTCAATCTTTTGAACAAATAAAAACCGTTCTAACTGAAGGAATTACAGAATTAGAAGTTTCAAATAAATTAGGCTACTTAATGCGTTTATTTGGAGCTGAAAAAGAATCATTTGAAACAATTGTAGCTTTTGGTACAAATGCTGCTGAACCTCACCACCATCCAACAGATCGTAAACTTGCTGATGGTGATATTGTTAAGATAGACTTTGGTGCTCAATATCAAGGTTGAGCAAGTGATATCACTAGAACATTTTTCTTTGGAAAACCAAAGAATCCAAAACTTGTTGAAATCCTAGATATTGTTAAAGAAGCTCAAAAATTAGGAAGAGAAGCCGTAAAACCAGGTATTGCTACAATAGAAATTGACAAAATTTGTAGAGATTATATTGAAAGCAAAGGATATGGTCAATACTTCACACACTCAACAGGACATGGAGTTGGAATCGATGTTCATGAACTACCTGGAGTAGGAAGAAACCGTGGAGATGCAATATTAGAAGAAGGTATGATTATTACTGTTGAACCTGGTATATATATCGAAAACGAAGGTGGCGCTAGAATAGAAGACACTATTTTGGTTACAAAAGACGGCTCAAAAGTATTAAGCAGACCCGAAGATTATGAAAAATAATTAAATAAAAAAAATGAACAGCTGGAAAATCCGGCTGTTTTTTTCGCTATATTTTCTTTATATTTAAATATTTCTAATTATCAATGATAAAGTTAAATCAAGGAGAATAAATTATATGAACAAAGAAGAATATTTAGCTTATTTAAGTTATATAAATAATGGCAAATGGCACCAAATCTATAATGATTTAAAATCACAAATAACATGTCAAAAAACAAAATATGCTGATTTTTGTAGTGACCAAGCAATCAAAAACAAATCATACATAACAATTTTTGATAGTGATTATCCGCTATATTGCAATAATGTTTGAAATCCACCGTTTGTCCTTTATTATTATGGAAATAAACAACTATTAAAGCATAAAATTATTATGATAACTGGAAATATTTCTATTTCTAATATTATCGAGGTTAAAGATTCTATTAAAAATTTTGGCAAAGAATATGTCTTCTGCAACGAAGCATGAAAAGGTTTAGACATACAATTAATTGACTATTTAATTTCAAAAAATAAAAATATTATTATTATTTTAGCATGCGGAATTGAATACGCTAAAAATTCTTTGCCTAAGAAATGATTCGATTATAGCAATTTGCTATTCATTTCTGAATACCCTAGCAATTATCACACAAGTAAAATAACGCTAAAAGCGCGAAATAGAATTAGCGCAGCAATTTCTAATTATTTAGTCTTACTCTCTTGTTATAAAAGCACCATGAATAACCTAATAGATCAATTCTTAAATATTGGCAAAGAGGTTTATTGCCTTGATGATATTCAAAATAATTTGAATGAAGATAATTTCAACAAAAACTTAATTAATGAAGGAGCAGGAAAAATAACTTCAATAACTGATTTAATATTAAAATAGAATATTATCTTTTTTATTATCCTCTAAAAGCATTATTATTTCATCTTTGTAAGGACTTTTATTATTAATAAACGGTGTTTCTAAAATAATGGGAATATTATCGAAGTCTTTATCATGAACAAATTTTTTCAATGTTTCTAAACCTATATGGCCTCTATCAATGTTTGCATGACGATCTTTTTTTGATCCAAGTTCATTCATGGAATCATTTAAATGAATAACTCTAATTAAAGGCAATATATTTTGTTGTTTTATATAAGATTTGAAATTCTCATAATCTTTTATATCATACCCAGCATCTCAGGCATGGCAAGTATCAAAACATATTGCAATTCTTTCGGAATTAACGGATTTTATCACGTATAATAAGTCATTGAAATTGCTACAAATTTGACTACCTTTACCCGACATTATTTCAAGGCATATTATTACGTCCTTAGTAGATTCAATTATCTTTTTAAGAGATTGAATTAATGATGCTAATGCTTCGCTTCTGCTATATTCAACTGATGAACCAGGGTGTAAAACTAACAATTTAGCACCAAAACTATTCATTCTTTTAATTTCACTACATAAAAATTTAATCCCAAAATCTGCCTTTTCTCTATTGGCTAAATTTACTATATATGGGGCATGAACCACAATATCAGATACAGGAATAATACTATTGCATTGCTTTAAATATTTTTCAATATTATACATTGAACTATCAACCCTAAAAGCACTTTGAGGTGCTCCTAAGTAAATCATAAAACAATTCTCGCCATTATTAATAGCTTCATTAAATGAACCTATTAAATAATCGGGTTTTTTAAATGATATATGGCTTCCTAATTTAATCATAAATACTCCTTAAATTTATAAAATTGCCTAATCTATTAATATTTAATTATATTAAATTATAAAGTTTAAATGCTCTTTTTAAATCATTCACAAATTTAAGAATAAAAAAATAAGGCTACACGTAATTCTTGATTTATGAACAGCCTTATTAATTGTATGTGTTTGTTTTAGTCTAGATCATAGCTATCGCTATTAAAGAATTCCTTCAATTTTCTACGTTTTTGAGGATGCTTCAATTTACGCAAAACTTTTGTTTCTAATTGTCTAACTTTTTCTTTCGAAATGCCTAATTCTTTTGAAAGTTCATCTAAAGTATGAGGTTTGTATTTGTTTCCCTTTAAGTCGTTAATTCCAAAACGTTTTCTAATTAAATCCCGATCAGCTTGATCACTTAACCCTTCTTCAATCATTTCATTTATAATAACACTTAACTCTTCATGGCTTGCATATGAAGTTGGGCTGATTACGCTTTCATCTTTAACAAAATCTGAGAAACTAGAATTTTCTTCTTTTCCAATGTTTTTATCCAAACTAATTGGATCAATATTAATTTTTCTAATATATCTTACTTTTTCAGCAGTAAAATCTCCACCGTATTTTTTAGCAATTTCTTCATCTGTTGGAGGATATCCATTTTCTTGTTGCAATTCACGTTCAATCTTCAATATTTTATTTATTGTTTCAACCATATGAACTGGCACTCTCACAGTTCTTGCTTGATCGGCTACTGCCCGTGTTATTGCTTGTCTAATTCATCAAGTAGCATATGTTGAAAATTTAAATCCTTTTGTGTAATCATATTTTGAAACTGCCTTCATTATTCCGGCATTTCCTTCTGATATCAAATCAATAAAATTCAATCCACGATTTTTATAACGCTTTGCATTATTGATTACTAAGCGGAGATTTCTTTTGACTAACATATCTCTAGCTTTTTTATATTTATAATAATTATTTGCTTCTTTAGCTTCTTGCATTTTTTTTGCTAGCGCTTGTTCTTCATTTGTAGTTAGCAATTTACCATATTTGCCAATTCATCTCATATATCATTTAACAATGTCGTTGGTTTCGGTTAGCTTATTAGTTAATTTTATATCATCTTGATCATAAACTTTTTCTTCACTCAACTTTATATCTTCATCATTGTATTCTTTTAGCAAATTGTTAGCATCGAAGTCATCGTATGATTGTTTTTTACCTTGCTTGTGGTTTGTTTGATAATCGTCATCTTCATATTCATCAAAATCTTCGCCGTAATAATCATTATATTCTTCATCATCAAGATCATTAAATGCCTTTTTAACAAAATGATTATCATCTTCGTCAAAAGACATTTCTGAGTCATCTTCTTCCTTGCTTCTAATTGAACTATCGTCAAATTCTTGATCAATATTTATATCTTCGTCAAGATTTTCTTCTTCCAACTCTTTAATTTTTGAAGGATCAAAATCTTTTTCTTCCTCTTCTGCGTCATCGTCATCATTATCATCATCTTTAGTTGTTGCGTGAGCAACTTCATCATCTAGCGAATTTATGTCAATATCTTCAATATCTTCGTCATCAGTATCAGATGAGACTAGATTAGCTGCAAAAAGGCTTTCAAGTAAAGCTTCTGCTTCTTCGTCATCAACGTAAAGGTTCTTTTTGTTTAGGAATTCAAAAATTTCATCTTGAGTAAAAGCCTTTTTATCCTTATTTTTCTTTTGGACTTTTTTTAACTCGTCTGCAACAATTTTAGTTATACACTTAACTGATGATTCTACATTATTTTTAGTATCTTGCATAATTATTTCCTTCTGTCCAAATTAATTATTAGGTCTTTGGATTGGCCTGTTTCTTTCATTCAATTATTTAAATTTTTATCTAGTTCTTCTTTTGTATATTCTTTGTTTTTAAGACTCATAATTTCCTTGAATAAATCTAAATATATTTGTTTAGCATTATCTTCAAGATCCATATTGTTTGTAATATATGATTCAAAATTTGTTTCATCTATTGATTTATTATTTTTTATGCTGTTTATTAAAAAACACAAAAACATTTTATGATTTTTACATCCATTAGAAAATGCCATTCTAGCAAAATCAGCAAGTTGATAGTTAGCAATGTATTGTGGGTGCTTTATAATGGTTTTTATTATTAAATAAAATACTAAATTTCTACCAGTTATTAATTTAGGCTGTTGTATTAAAGGTTCTTCGAAAAAATCAAGTCTATCAAACGAGGGATCAGAATATACAAAATTAAGATCTTCGTATTTTTTGTAATTTGAGTTTTTAGAATAATTTTTTTTATTATATTCATTATCTAGACTAGGTTTTAAATTATTTTTTGAAATAGGATGAAGTGGGAAGTTTTCTTTACCATATAAGAATAAATATTTGTTTCAAGTTTCAATTGAAAAAATTTTATTTTCAATCAATTTTTCTTTTAATTTCAATATTAATGGTTCTTGCAAATAAATCACAAATTCAAACAATTCAGCATTTAATTCAACCTTTCTTGATTCGTTGGTTTCCAATTGAAATACTTGTTTAAATTCATTGAATAAATACATAAATAAATCGACAGGACAACATGCTATCTCAGACAATGTTTTTCCTTCGTCAATATTATATAATTCGTCAGGATCTTTGCTATGTTCATTCAAAACAAAAGAAACTTTTAAATTATATCTTTTTTGATTTGCTAATATTATTCTTAAATTTTTCTTAGTAGCATTTTTGCCGGCAAAATCATTATCAAAAAACAAAACAATATTACAATTTCTTAAAGATTCTAAATTTTGTATAGATAAAGTTGTGCCTAAAAGTGCCACTGCATTTTTTATGTTTATTTTATAAAGAGCAATGCAATCAAATTGCCCCTCAACTAAATAAACACTTGATTTCTTTATAATTTCATCCAATGCATGATAGTAATTAAACATAACTTCATGCTTTTTAAAAACTATTGTTTCAGAACTATTTAAATATTTTGGTTCAACATTGTTATAAATACTTCTTCCAGAAAAAGCAACAATTCTTCCATCCTTATTTTTGATTGGAAACATCAATCTATCATTAAAAAAGTTATCAAAATTGTTGCTTGCTATCAATGATGAATTGGCTAAATTAAATGATGAACAATTTTTAGCAATTAGCATATTACATAAATTTTTATCTTTATCAGCAAAGCCAATTTCAAATTCCTTAATAAGTGGTTTTGTTAACCTTCTTTTTTTAAGGAACAATTTTAATTGCTCATTTTGATTAATTTCTAAAATTTCAAATTGAAATATATCGCTCGCTATTTGATTGATATTGTATAAATCTTCTTGTTCTTTTGTTGTGGTAGTTATACTATTACTAAACCTTGTTAAATCGATATTTAAATTGAATTTTGAAACAAGCTCCTTGATACTTTCAAGAGGGCTTTTTTTAGTATAAAGTTCAATGAAAGTTATTAAATTTCCGCTTTTAGAGCAACCAAAACATTTAAATATTTGTTTTTCTTGTGACACAACAAAAGAAGGAGTTTTTTCGCCATGAAATGGGCAACAAGCCACATAATTCTTTCCTTTTTTTGTCAAAGAAATATATAAAGAAATAACATCAACAATGTTTAACTTTTGTTTAACAATTTCAAATATATTATCTTCTGCCATTTAGCTCCTTTTATTTTTTATAATTTTCAAAAAACTTAATTAAGTTGTCTAATTCAACTACATGTTGTTTCATAGAATCGCGTTCTCTGACAGTTACTGTATTTTTTTCTAATGTGTCAAAATCTATTGTTACTGCAAATGGTGTACCAATTGCATCTTGACGACGGTAACGTTTTCCTATCGAACCGCTTTCATCATAAATTAATCTTAATTCAGTTTTTTGCAATAAATTATCAAATATTTCTTTAGCCTTAGAGTTAAGTTTTTTCATTAATGGAAAAATCGCAACTTGATATGGTGCAATATTGAAATCAAGTTTTAAAACAATTCTTACATCATTATTTTCTAGTTCTTGTTCTTCATATGAATCTACAAGCAACGCAAGCAATAAACGATCTAAACCAATTGAAGGTTCAATAACATGAGGAATTATTTTAGTTCCATCGTCTAACAAATATTCAAGAGATTCATTTGAATATTTCATATGTTGGCTTAAATCATAATTGCCACGATTTGCAATACCAAGCAATTCTCCTCAACCAAATGGAAACAAATATTCAATGTCGCTGGTGCCTTTTGAATAATGGCTTAATTCTTCTTTTTCGTGTGGTCTAATTCTTATATTTTCCGACTTTAATCCCAAAAGTGTAACAAAATCAACGCATTTTTTAATTCAATAATGGTAAAACTTTTCATCTTCACTAGGATTGCAGAAATATTCTAATTCCATTTGTTCGAATTCCCTTGTTCTAAAAATAAAATCCCTAGGTGTAATTTCATTTCTAAAACTTTTACCAATTTGGCCAATTCCAAAAGGTAGCTTAGCTCTAGTTGTTCTTAAGACATTTTTAAAATTTATAAAAATTCCTTGGGCTGTTTCAGGACGCAAATATGTTTGATTTGCTGCTGTATCAATTGCACCCATTTGGGTTTTAAACATTAAATTAAATGGCCTAATTGAAGATCATTCAGTTTTTGAACCATCATATTCCTTAACTTTTTCTTTAATTATTTTTTCAAGTTCTTCAAATGTTGCTTTTTCAACATTAATTTCTGGAAATAAATCTTTAACAATATGGTCTGCCCTATAACGTTTTTGATTTAGTTTGTTTTCAATTAAAAAATCTGAAAAATTGTCTAAATGCCCACTGGCTTTTCAAACTTTTGGATTCATAATAATTTTTGAATCAATTTGAACATTTCCTTCTTTGGCAATAAATTCTCTTACTCAATATTTCTTAATCGCAGTCATAATATTAACTGCTAGTGGCCCATAATCCCAGGTGTTTATTAAGCCTCCATAGATTTCTGAATTTTGATAAACAAAACCAGAAACTTTTAAGTGATTTATTATATTTTGAATATTTTTTTGTATTTTATCTCCCATAAAAACTCCACAATTTTATTTTATATAAAATAACAATGGAATTTTACCATATTTTTCCTTTTTCATTATTCAAAAAATAAAAATGATCATTGCATTTTTCGAAATTGTTTCAATAATATATTGCTTTGTTAGTATAATAATTACATGTTAGATTTTATTCAAAAAAGAATGCAAAATTCAATACAAAAAATTAATAAAAAAATGTCTATTAATGAAGAAGACATTTTAGAAATTTTAAGAGAAATTAAAATATCGTTATTGGAGGCAGACGTTAACCTTGAAGTTGTTAAAAACTTCACTAAAGAAGTAAAGGCAAAAGCCCTTTCTTCTCAAATTATTGGAAAGTTAAATCAGCAACAAACAGTCCTAAAAATATTCAAAGATGAATTGACTAGAATTTTAGGTTCGAAATCAATTGATATTAAAACAAACAATAACCCTACTAAAATAATGATGGTAGGATTGCAAGGTTCAGGTAAAACTACTACTGCTGCAAAATTGTCTGTTTATTTCAAAAAGAAGAATTTGGCAGTAAACCCTTTGCTTGTTGGTGATGATATTTATAGACCTGCTGCCAGAGAACAATTGCAACAATTAGCAAAACAAACTCAAACTGATTTCTTTACTATGGAAGAAAACAATGCAATTAATATTGCTCAAAAAGCAATAGAATTGGCAAAGGAAAACAAAAACGATTTAGTAATAATCGATACTGCCGGAAGATTGGCAATTGATGAAAATTTGATGCAAGAACTAAAAGATATTAAAAAATATACTCACCCTGATTATATATTTCTAGTAGTTGATGCAATGAGCGGACAAGATGTTATAAATACAGCAAAAATTTTCCACCAAGAACTTAATTTAGATGGCACTATTATCACAAAATTAGATAGTGATGCTCGTGGTGGCGCAGCCTTGTCGATTACTCATTTATTGAACGTTCCAATAATTTTTATTGGTACTGGTGAAAAATTAACTGGTTTAGAATTATTTCATCCAGATAGAATGGCAGATAGAATATTGGGCATTGGTGATGTTCTAACATTGATTGAAAAAGCTTCGGAAGAAGTTGATGAAAAAATGATGAAAAAAATTGGCTATAAAATGCTTTCTGGCCAATTCAATTTAATGGATTTGATGAATAGTTTAGCTCAAATTAAAAAACTAGGTAAGATGAAAGCAATCTTAAGAATGATTCCGGGAATGGCTGATAAGGTTTCAGACGAAAAGATTGAAGAAGCAGAAAATAAATTTAGAATTTATACTTATTTAATTAATTCAATGACGAACTTTGAGAAAAAAAACCCACGAGTTTTAAAAAACCCAAGTAGAAAGCAAAGAATTTTGCAGGGTTCTGGTAGAAGCCCAAGAGAATTTAATATGCTAATTTCGGATTTCGAAAGAATGTCTAAGCAAATGAAAGAATTAGCAAACGGAAATCTAAAATTAAAAAATGGCTTGGATGAATAAAAAAAGTGCCTATTGTTTGGCACTATTTTTTTAAACTAAATAAATAACAGCTTGAAATATAAAATTGCAAAATAATAACTAAAAAATTCTTTAAAAATAAAAAAGCACTTATGGGTGCTTTTTATAATTTATTCTTATTTTTAATTGAAATTAAGACTAATATTGCTTTTGATATTTTTAGACTTGATAAAACATATCTATCAAAAATAATAATATTTCTTAATTTAAATATTGAATTTAATTTTTTAGTTTTTACAATTAGGTTATATGGAGAAATAGTTTTAATTTTATTGATGAAGTGATTAAATAAATTATTTAAAAAATATGAATGGTTTTTAAATGTTTCTAAAATATATTTGATATTTTCAGAGGCTATTTTTTTATCGCGAGGTTTTAATTCTTGACAATTTTGTTTTGAAAAATAAGTATTTAAAATGAAATTGATTGTTTCTTGCATTTCTTCATCTCTTGAAATTTCAATAAATGCCTTATCTAATAAATAAATTAAATTGTATTTATTATTTACTATTAAATTAGTTCATAATTCATCAAAGGTTTTTAATTTTGCATAAATATCGCTATAGTTTAAAAGTGTTTCTACTATAAAATAAATAAATTCCTTAAATGTATTCAATTGAGACAAAAATTTCTTAATTTCTATATATTTTTGATTATCGTTGAACGAAAATAAAAACATTAGCAAATCTGTATTTAATGATTCTTTTGCAAACGTTAGAGCAATTTTTTTGATTTCTTCTTTGTTTTCTTCTATAATTCCTGCACTAAAGAATTGTTTAAATACATCATATATTAAATATTGATAATTGCCTAGAATATAATTTCATTGTTCACGCAAATCAAAATTAATAATTGTTTTTTTAGTTAAAAATACATCTTCAAGTCTTGATTGTAATCTAAATAATATATCATCAATTTTTCCATCTTTAAATAATGTTAAAAATATAGCTTTGCTATTTGTTTCGTTGCTTAAAAATGCATCAATCAACTTATAAAATTCATACTTAGTTTTTGCACCAAAAAATTTATTTTTTAAGTAATGTCAAGCAATGTTATGAATATCATAAGAAGAATATCTATTTATTAATTGAATAAATTCTGAATTATTTAAATGTTTATATAAATATCTTTTTAGAGATATTTCTAATTTGCTATCTATTAATATATTGAAATTCTTATTAATTCCATAGATATTTTCTAGTAGGTCTAAATCATTGTTGTATAGTGAGAATAATTGTTGATCTTGGTTTTCAAGTGTTTGATTTCAATATTTAGAATCACTACAATATTGAATTAAATTAGACATTTTAAATTCACTAGTTAAACTTAATTTAGCAAGTAAATCATATGCAACTTTTTTATATCCTTTTTCGGTGCAAAACCAACTAAATAAGTTTTCATACAAATAATTTTGGTGTTCTTTTCAATATTTTTCTTCATAAATATCTACATATAAACATTGGCATTTTTTAGCTACTTCTTTAGCGACATTGTTTAATAAATCTTGTATCATTTCAACCAAATCAAATTCATTGTTATATGAAGAAGAAATGTAAATCGAATTAATTAAATCTTGCAAGGCCATAAATGGTTTGGCATAATTTGTTACCACGATATTTGCTAGTGGATTAATTTTTTGAATATATTCAACAAGCTTTTGATATTTTGAAATAATTTCTTTGCCGATATTTTCAAAATCTTTTTTTATTTCAATAATTAAATTGGTTTTTGTTTCATTTTTTGCTGTTATTAAATTAGTTAATTTATAAAATGGCATTTTAAAAATTAAATCGCTAAATCCAGCTGTAATTGTTATAAAATTTGCCTTTGAAATAGTTTCTTTATAAAAAGAAAAATTTTTTTTAGAAATGTTTCAATTTAAAAAAAATTCATCAAAAAAATTCTTTGAAGGATTTAAAACATGCCAATCAATTGATTGAAGCAAATCAAGACTTTTTGAATTGGCAATTAAATCATTTTCATTATTTGAAGTTAAGGCGTAAATTATATCTAAATTACCTGCTGGTATTGCTAGATTGTAAAAACTTGTTAATCTATAGTTTAAATCTCGCAATTTTAAGGCTAAAAATGATGGATAACCCAAACCATCTATATTACCATTTGATAATTTTCCATTAGTATTAAACCCTACTTTGCTATTAAATCCCGCTGCAAATGAATCTCCTAAAGCAACATATTTAATTTCGTCATTTAAATTATATTTTTGATTTTCAGCCATTTTATTCCTTTTCTTGACATTGATCATAAACAATATTATTTAGATTATTAAAATAAGAGATTAATTGAACTATTGCTTTTTTTATATCGGGTAATTGATTTTTTAATATTCAATTATTATTTTTAGCATATAAATTCAATTGGCTAAAGATTTCTTCTTCATCTTGACTTGGTTGCAATTTTATATCAATAATTTTTTGAGGATAATACTTAGATATTAGGCGGTAAGTAGCAACAATAACTTCATTTATATTTAAAGCTTCTAACTTTATTGAACTAACTACAGCTAATTTGGCGCCAGTTTCTTCATCTTTTAGTTTTGGCATTAAAAGTCCTGGCGTATCTAAAAGTAAATAATTTTTGCAATTTATTCATTGCTCTGACTTTGTTACTCCGGCCATTGCACCTACTCTTGCAACTTTAGAACCAGCTAATAAATTTATTAAGGTACTTTTACCAACATTAGGAACGCCGAGCACAACACATTTTAATGCTGGTGTTAACTTATGTTTTTGCGCATTTTTTTCTTTTACTTTTAAATAAATATTATTAAGAGCTGAATTTATTTTTTTATAGGCTAATTTGTCTTTCAAATTAGTTGTTACAACAACACCTTTCTTCTCATATTCATTTATAAAAGGTTTTAATTTAGATAGATCTGCTTTGTCGCTTTTGTTTAATATAAATAAAATTGGCTTATTATTAGCCATCTTATAAATATCGTTATTAAACGAAGTAAGAGGTAAGCGTGCATCTAAAACAATTATAAATAAATCAAATAATCTTATTTTTTCATTTAATAATCTAATGGTTTTGGCCATATGACCAGGGAATCAATTAAACATAAAATTTATCAATCTTTCCTTAAAATAAAATCCTTCCCTTTTTGACTTTCAAATTTTGATAGTCCGGCGAAATCATTTTGTCTTAATACTTCAAATAGTACTATTGCAACACTATTGGCTAAATTTAAACTACGACATTCATCAAACATTGGGATACGCAAACAATTAGGAATATTTGTTTGCATAATTCTCTTAGGAATTCCAGTACTTTCAGTTCCAAACATAATTCAAAGTTCACCATTTGTTTGCAATTCTTGTTTGTAATCAACATCTACGTAATTTTTTAAACCGTATCTTGTTACATAATAAATATTTTTATCACCATATTTTTTAAAAAATTCATCATAATTTTCATGAACTTCATGTTGAATTTCAGTTAAAAAGTGTCCAGCGGCCTTTCTTTTCATTCAATGAGGATGCAGATCAAAGGCAATCGGTTTAATAATATGCAATTTTGCATTGCCAGCAAAGCATGTACGAATTATATTAGATGTATTTGGGCTTATTTCGGGTTGGTATAAAATAATGTTAATCATGTAAAGTATTTTACTACAAATTGGCATTAAAAATAGTAGATATTGTAATTTTAATTATTTTAATAATTAAAAAATAAATTTATATAAATAAATTCAAAAAATCGGATTTTAAAAAAATTATAACTAAATATTCATTTTTATTGTAGCTACATTTTTATCTTAATATATAAAACAGAATAATTACAGAAAATAAGAAAAAATAGCGAAAAAATCGCTTTTTTTCTAAACTAAAATAAAAAGCACATTCGTTGTGCTTTTTATTTGCTTTATTTTCTGATGTTTAAAGCTTTGATGGTTTCTAAATATAAGTTGTAGTTTTCGTCTTTTAAGTAATTTAGTAATGCTTTACGGTGATTAACTTTAGCAATGAATCCTCTCATTGAATGTAAATCTTTTTTATTTACTGCGAAATGTTCTTTTAAACTTTCAATATCTTCAGTTAAAATTGCTATTTGTACTGGTAAATATCCAGTGTCTTTTGCATTACGTCCGAATTTAACAACTAATTCAGCTTTTTTTTCTTTTGATACCATATTTTTCCTTTCATAGCCTAGCAATAATCTTAATTATTACCAAGGGATAAAATTGTTATGAATATATTTTAACATAAAAATAAAAAAAATACGCAAATGCGTATTTGGTCAAGTTAATGAAATGGTGCGAACGAATGGACTTGAACCATCGACCTCACGATTATCAGTCGTGTGCTCTAACCAACTGAGCTACGCTCGCATATAAGGAGTTTTAAAAAACTCCATAATATTATAACACAAAAACAAATTAACGTTTTGAAAATTGTCTTGCACGTCTTGCTTTTCTTAAACCGAATTTCTTACGTTCTTTAACTCTAGCATCACGTGTTAAAAATCCTGCATCCTTTAATTTGTTTCTATATTCATTATTAGATGCTTCTAATAATGCTCTAGCAATACCTAATCTAATTGCTCCAGCTTGTCCTGTTAAACCACCACCATTAACATTTGCAAAAACATCAAATTGGTTAGCAGTTTCAGTTACTGCAAAAGGACTTAAAGCATCTTTTATTAAAATTTCTGAATTTAAGTATTCTTTTGCAACTATTCCATTAATAATTAAATTTCCACTACCTGGTAAAATATAAACTCTTGCTACTGAAGATTTACGACGACCTAAACCATAGTATCTTACTTTATCTGCCATAATTATTTAACCTCTAATTTAACTGGTTGTTGTGCTTCTTGTTTGTGTTCAGAACCTGCATAAACATATAAATTTTTAAATTGTTTACGACCTAATTTGGTGTGTGGAAGCATTCCTCTAACAGCTTTTTCAACTAATAATTCTGGTTTTTTTGCTCTTAAATCAGCAGCAATAATTTGTTTTAATCCACCTGGGTATCCAGTGTGGTGGTAATATACTTTTATTTCATCTTTTTTAGCAGTTAAAAGTACATCTTTAGCATTGATCACAACCACATTGTCGCCCATATCAACATTAGGGGTAAAAGTTGGTTTATTTTTACCTCTAAGTACAGAAGCTACAAGTGCTGAAAGTCTACCTAAAGGTACGTTGGCTGCATCGATAATGAATCATTTTTTATCAACGATTTCTTTTCTAATAATTGTTGTTTGACGCATACTTTCTCTTTCTTATAGTTTGTTATTTTTATAAAATGCTTACCTATTATACCATATATAGTTATTATTAAAACATTTTTATTTAAGTTTTAAATATTTAAATAAAACCTGTTTAATTTAATTTTGAAAGCATGTAAATTAAAGCGGTCTCAGCCCTTAAAATCGTTTTTGTTAGTGAAACAACTTGAACATTGCAAGATATAGCATAATCAATTTCTTGTTGGCTAAATCCGCCTTCAGGGCCAACAATAATTAAAGCATCTTGGCAATCTTTAATTAATTCGTTATTTTTTTTGTTTTCATAAGCTAATACTTTATTTTTAATATCCAAGACACAAAGTTCTGAAAATGAAATTGGATCATGAAGTCTTGGGATACAATTTCTAAAACTTTGTTGGCAAGCTTCAAAAATTATTTTTTCATAACGTTGCTTTTTAGAATCAAAATTTAAATTAGATTTATCAACATATTGACTTTGAAATACATAAATTTCATAAGCACCAAGTTCAACTGCTTTTTGCAATGCTATTTCAAAATGGTTTTGTTTAATTAAAGGAATTGCAACACATAATTTATAGTCAAGTTCATTATTAATATCTAACTCTTTAATTAATTTAGCACTATTAGGAAAAACAAATTGACATTCATAAAACTTATTTTCATAATTTACAATAATATTTTCTTTATTAAAGCGCAAAACCTTAAAATGCTTTTGAATTTCATCACTAAAATAAAAAATTCCATCTTCTAATTTGTTAGCAAAAAATTTGTACATATAACTATTTTAATATAACCAACACAATAAAACAAAAAAACCCGTAACTTTTTTATAAATTACGAGTATAAATTTTTTACTAAACAATTAATTATATGTAATCTAATTGCTAAAAATTATTTTATTAATAATTCAAATTAATTGTTAGATGAAATAATTTTTGTATTTGTTTCGTCTATACCAATATATTGTCAACCTATATTACTTGCATCACCTTTATAAATTACTTCTTTAAGGTTTTTGCAGCCAAAAAACGCACTATAGCCAAATTTTTTTACTGAAGCAGGAATAGTAATAGATTCAATGTTTGTATTGTAAAATGCCTCATCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTTGACAGCCAAGAAATGCACTATCATCAATTTCTTTTATGTTGTTTGGAATAGTAACTTTTGTGGTTTCTTTATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATGTGAAATTATTGAAAAATTAAATGCCCTAGCGCCGGTTTTTTCAAGACCTTCATTTAAAAATACTTCTTTAAGGTTTTTGCAATCATAAAACGCATAATCGCCAATTTCTTTTACTGACCGCGGTATAACAATATATTCAATGTTTATAGTTTTAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTTTACAACTAGAAAACGCACTTTTGCCAACTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTCTGTATTATTAAATGCATAATCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTTTACAACTAGAAAACGCACTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGTATTATAAAATGCCTCAGCGCCAATTTCTTTTACTGTACTAGGAATAGTAATAGATTCAATGTTTGTATATTTAAACGCCTGAGCGCCGATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAATGTTTCTGCAGCCAGAAAACGCACCTTCACCAATTTCTTTTACCGAACCTGCAATAGTAACTGATTGAATAACACTATTAAAAAAAAAGCACGATTACCAATTTCTGTAATATTTTTGCCTTGATATTCCTTAGGAATTGTTACTTTAGAATCATTTCCAATGAATTTTGCAATTTTTCAAGTATTTGATTTAGTTAGTTCGAATTTGAATTTTTTATTTAAGTTTGATGAATCTTCTTCTTTATTAGGATTTACTGGTTTGGGTTCTGGTTTATTTTGATCGGGCTTTAAATCATCTTCTTTGGTAGGATTTACTGGTTTGGGTTCTGGTTTATTTTCATCAGGCTTTAAATCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTTTATGATCGCAAGAAATAGCTGCTAATGGAAATATAGCAGTTAATGCTGAAATACTACTTAATATAAGTCATATCTTACTATTTTTTTTATTTTTCATATTCTTTTCCTTTGTTATATTAATTATTATATTTTATATATCTATTTAAAGCAAATAAATTATTAAAAAAATAAAAAAATTCTTTTTTTGAATGAATTTGGAAGTGTAGAAAAAATGTGGAGTTAATTCCAAATAAGGAAAAAATAAGTTTAAAAATTTAAAAATTTTTGAAAGAAATTTAAATTAGGAGAAAAATTATAGTAAAAAATCTATGACAAATTAAGCAACATAGCTATAAACAAAATTTTAAAACCAAAAATAGTTATTATCAAAAGCTAAAAACAAATAAAGTGCTGCTTGAAGTTTTTATATTATTAAATCCTTAAAAAAATTCCATCTTCTAATTTGTTGGCAAAAAATTTATACATATAAGTATTTTAATATAACCAACACAATAAAACAAAAAACTCGTAACTTTTTTATAAATTACGAGTACAAATTTTTTACTAAATAATTAATTATATGTAATCTAATTGCTAAAAATTATTTTATTAATAATTCAACTTAATTGTTAGATGAAATAATTTTTACCTTTGTTTTGTCTATACCAGTATTTTCTCAATTTATATTACTTGCATCTCCTTTATAAATTATTTCTTTAAGAGTACCGCACCAAGAAAACGCTCCTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATATATGTATCTCGAAATGCTTCAGCACCGATTTTTTCAAGACCTTCATTTAAAATTACTTCTCAAAGGTATATGCAACCATAAAACGCTCTTTCGCCGATTTCTTTTACTAAACCAGGAATAGTAATAGATTTAATGTTTGTATCTCGAAATGCTTCAGCACCGATTTTTTCAAGTCCTTCATTTAAAGTTACTTCTTTAAGGCATTTGCAACCAGAAAATGTACTTTTGTCAATTTCTTTTACTAAACCAGGAATAGTAACAGATTCAATGTTTGTCCTCTCAAATGCACCGGCACCAATTTTTTCAAGTCCTTCATTTAAAGTTACTTCTTTAAGGTATTTGCAACCAGAAAACGCACTTTCGCCAATTTTTTCAAGTCCTTCATTTAAAAATACTTCTTTAAGGTTTGTGCAATTATAAAACGCATATTTGCAAATTTCTTTTACTGAACCAGGGATAGTAATAGATTCAATTTTTGTATATTGAAATGCCCAATCGCCGATTTTTTTAAGACCTTCATTTAAAATTACTTCTTTAAGGCATTTGCAACCAGAAAATGTACTTTTGTCAATTTCTTTTATTGAACCAGGAATAGTAACAGATTCAATGTTTGTCCCCCCAAATGCACCAGCGCCAATTTTTTCAAGTTCTTCATTTAAAGTTACTTCTTTAAGGTTTGAGCAACCAGAAAACGCGTTATCTCCAATTTCTTTTACTGAATTAGGAATAGTAATAGATTTAATGTTTGTATCTCGAAATGCCTCAGCGCCAATTTTTTCAAGTTCTTCATTTAAAATTACTTCTTTAAGGTATTTGCAATTAGAAAATGCTCTTTTGCCAATTTCTTTTACTGAACCAGGAATAGTAACTTTTGTAATTTTTTTATCCAAATATGCTAATATTTTTTCATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATGTGAAATTATTGAAAAATCAAATGCCCAATCGTCAATTTCTTTTATTGAATCAGGAATAGTAATAGATTCAATGTTTGTCCTCCCAAATGCACCAGTGCCAATTTTTTCAAGTCCTTCATTTAAAGTTACTTCTTTAAGGTTTGTGCAATTATAAAACGCATATTTGCCAATTTCTTCTACTGAATCAGGAATAGTAATAGATTCAATATTTGTATCTTGAAATGCACCAGAGTCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAATGTTTCTACAACCAGAAAACGCACCTTCGCCGATTTCTTTTACATTGTTTGGAATAGTAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATCTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATATGAAATTATTGAAGAATTAAATGCAAAATCGCCAATTTCTTTTACCGAACCAGGAATAGTAATAGATTTAATGTTTGTATATTTAAATGCCTCAGCACCAATTTTTTCAAGTCCTTCATTTAAAGTTACTTCTTTAAGGATTTTGCAATCAGAAAACGCATATTTGCCAATTTCTTCTACTGAATCAGGAATAGTAATAGATTCAATATTTGTATCTTGAAATGCACCAGAGTCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAATGTTTCTACAACCAGAAAACGCACCTTCGCCGATTTCTTTTACATTGTTTGGAATAGTAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATCTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATATGAAATTATTGAAGAATTAAATGCAAAATCGCCAATTTCTTTTACCGAACCAGGAATAGTAATAGATTTAATGTTTGTATATTTAAATGCCTCAGCACCAATTTTTTCAAGTCCTTCATTTAAAGTTACTTCTTTAAGGATTTTGCAATCAGAAAACGCACTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAACTGATTGAATAACACTATGAAAAAAAGCACGATTGCCAATTTCTGTAATATTTTTACCTTGATATTCCCTAGGAATTGTTACTTTAGAATCATTTCCAATGAATTTTGTGATTTTTCAAGTATTTGATTCAGTTAGTTCGAATTTAAATTTTTTATTTAAATTTGATGAATCTTCTTCTTTTGTAGGATTTTCTGGTTTAGGTTTTGGTTTATTTTCATCAGGCTTTGAATCTTCTTTGGTAGGATTTTCTGGTTTAGGTTCAGGTTTATTTTCATCAGGTTTTGAATCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTTTATGATCGCAAGAAATAGCTGCTAATGGAAGTATAGTAGTTAATGCTGAAATACTACTTAATATAAGTCATATCTTACTATTTTTTTTATTTTTCATATTCTTTTCCTTTGTTATATTAATTATTATATTTTATATATCTATTTAAAGCAAATAAATTATTAAAAAAATAAAAAAATTCTTTTTTTGAATGAATTTGGAAGCATAAAAAAATGTGTGGTTAATTTCAAATAAGAAAAAAATAAGTTTAAAAATTTTTGAAAGGAATTTTATGGGTGGCAATAAAGAATTTATGTTAATTCAGCAAGAGGCTAAATTAAACAATGATTAATAGCCTTGTGGATGCATTTGAAAGAAATTTAAATTGGAAGAAAAATTATAGTAAAAAAAATCTATAACAAATTAAGCAACATAGCTATAAACAAAATTTTAAAATCAAGAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATAGTTATTAAATCTTTTAAAAAATTCCATCTTCTAATTTGTTAGCAAAAAAATTTATACATATAAGTATTTTAATATAACCAACACAATAAAATAAAAAAAACTCGTAACTTTTTTATAAATTACGAGTATAAATTTTTTGCCTAATAATTATTATATGTAATCTAATTGCTAAAAATTATTTTATTAATAATTCAAATTAATTGTTAGATGAAATAATTTTTATCTTTGTTTTGTCTATACCAATATTTTTTCAATTTATATTACTTGCATCACCTTTATAAATTACTTCTTTAAGGTTTTTGCAATTAGAAAACGCACTTTCACCAATTTCTTTTACCGAGCTTGGAATAGTAATAGATTCAATTTTTGTATTTGGAAATGCACTAGCGCCGATTTTTTTAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCAATTACAAAACGCTCTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTATCATAAAATGTCTCTGCACCAATTTTTTCAAGACCTTCATTTAAACTTACTTCTTTAAGGTTTTCGCAATTAGAAAACGCACTTTCACCAATTTTTTTTACATTGTTTGGAATAACAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATTTTTTTATTATTTTTAGGAAGCGTAGAAAAATGTGGGGTTAATTCCAAATAAGGAAAAAAAGCAAGATCATAAAGGTCTTGCTTTTTTTCTATCATTCATAAAAAAAATAAGTTTAAAAAAACGAAGAGCACAACACAAGCACAGCTCTTCATACATCTACATAAATTATACCACGTGCTCTTCGTTTTTTTTAAAAAACTTAAAAATTTTTGAAAGGAATTTTATGGGTGCTTATAGTGAATTTATGGTAATGCAGCAAGAGGCTAAATTAAACAACGATTATAGCCTTGTGGATGCATATGAAAGAGATTTAAATTGGAGGAGAAATTATAGTAAAAAAATATATGACAAATTAAGCGACATAGCTATAAGCAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATGGTTATTAAATCCTTTAAAAATAAAAAAATCAAAATCATTGATCTAATAAATTTAGGGTTTAAAAAAACTTCTATAAAATCAGCTATAAAAAGACTTATTGAAATAAAAGTTTTAGATGGTGAGTTTTATAAAAATATAAACTTCTAAAATTAAAAAAATAGTACTAAAAAACCCTAAGGAAAATTACTGGATTTTAAATGGCTATGATGCCTTAAAAATCTTTCTTCTTAATGGTTTAAGTAATGCTATTTTATATGCAATAAATAGGCATAAATCAAAGCAACTAAATGCTAAAAAATTGCATTGTAAAATAAACTCAAGAAAAGTGATTTTACAAAATGCATATTATGCAAAATTTAACTGATCAAATAGCAAAATATATTTAATGAACAAAGCCATAGCCAATTATTTTGGAGTTGAATATTTGCAAATGTTTGGTATATTGCGTAGTCAAGAAAAAAAAGTTACAACATTAAAATTAGGAAATTTAAACATCAAATGTAATGTTTATAAATTTTCTACACAACGTTATTTGTTAATGCAAATTTAAAAGTTTTAAAGGTTTTTATAAGCAGTAATAAATGGCTCCCAGCATTGGGCTAATGTTTCTGCATTGTAGGTTCAAATCCTACGCGAGCCACCAAATAATATTTACATTTTAAATTACTACTTTTAAAAACGTTTAAATTTAAGTTTTGATAGTTACTGGCACTAAGTGTCTATTTTAGTCATAAATAAATTAGTCAAATTACTAATACAAATTAATAAGAGCGAATATGCGGTCAAAATTTTAAAGATAAATATATAGCAGTAATAAATATAAATAATTACTAATGTTTTAGAAGGGAGAATAAATGGATAAAGTATGAACTGAATTTAATTTTATTACTCCTAACACAATGTATAAAACCAAACTTGGTAATACTCACGATTTTAAAAGTTGAGTTGATTCTGGACTTGTGGTAGATTACATTACAAGACCAGATAAATGCGTTGTATTTAAAGAAGATGAAAATAATTATGATGCTATGAAACTACTTCTTACTAAGGAAGATAAAATAAATTGATATAGACAACATTTAAAAATAAATCAAAATTCTAAACAATCAGGTCTTTATAACTTATTAGATAATGATCCAAAGGATGTAAATTTAGAAAATGTCAAAAACGATTTAAAAAATTTAAAAGGTAATTATTGAGAATTAATCGTTAATCCTGGTGATTTAGGCTTAGATAATAATGTTTTATGTAAGGATAAATGACATGAAGTTTTAAAACCTAATTTTGAAAGATTTTTAAAAGCTAATGCCTTTGATTTAGAAAATGTTAATGTTTTTTATTCTATACATGGAAATACTGATTATCCTCATGTTCATATGTTTTGATATGAAAAGGAGCATAAACACAAAAAAGCCAAACTTAATTTAAATTCAATTAATGTTTTTGGCTATAAAGTGGGTCTATCTATTAAATACGATGAGGATTATCAAAAAATAAAGGAAGCATAGAAAAATGTGGGGTTAATTCCAAACAAGAAAAAAAGCAAGATCATAAAGGTCTTGCTTTTTTCTATCATTCATAAAAAAATGAGTTTAAAAACTTAAAAATTTTTGAAAGGAATTTTATGGGTGGTAATAAGGAATTTATGTTAATTCGGCAAGAGGCTAAATTAAACAAAAATTATAGCCTTGTGGATGCATTTGAAATAAATTTAAATTGGAAGAAAAAATATAATAAAAAATCTATGACAAATTAAGCAACATAGCTATAAACAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATTAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATAGTTATTAAATCCTTAAAAAAATTCCGTCTTCTAATTTGTTAGCAAAAAATTTATACATATAAGTATTTTGATATAACCAACGCAATAAAATAGAAAACTCGTAACTTTTTTATAAATTACGAGTATAAATTTTTTACTAATTAATTAATTATATGTTATCTAATTGCTAAAAATTATTTTATTAATAATTTAAATTAATTGTTAGATGAAATAATTTTTACCTTTGTTTTGTCTATACCAGTATTTTCTCAATTTATATTACTTGCATCGCCCTTATAAATTGCTTCTTTAAGGTATCAGCAACCAAGAAATGCATCAACGCCAATTTCTTTTACTGAGCCAGGAATAGTAATAGATAAAATTTTTGCATACCTAAATGCATAATCGCCAATTCTTTCAAGGCCTTCATTTAAAACTACTTCTCCAAGGTTTTCGCAACCAGACAATGTACTTTCGTCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGCTTGTATGATAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCATTAAGGTTTTGGCAACCAAAAAACGCTCTTTCGCCAATTTCTTTTACTGAGCCAGGAATAGTAATAGATTCAATGTTTGTATGGCGAAATGCCTCAACGCCAATTTCTTTTACTGAGCCAGGAATAGTAATAGATTCAATGTTTGTATATTTAAATGCCTCGGCACCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTTGCAACCATAAAACGCACCTTTGCAAATTTCTTTTACTGAATCAGGAATAGTAACTTTTGTGGCCTCCTTATCCAAATATGCTAATATCTTTTTATTATTTTTATCCATAAAAAAGTTATCTTCAATTTCAAAATTTTTATTATTTGAATTAATTGAAATACTTCGAATATCTCAAAAAGATAATGCACCTTCGCCAATTTCTTTTATTGAACCAGGGATAGTAATAGATTCAATTTTTGTACTTGAAAATGCCTCATCACCAATTTTTTCAAGACCTTCATTTAAACTTACTTCTTCAAGGTTTTCGCAATTAGAAAACGCACCTTTGCCAATTTCTTTTACTGAATCAGGAATAGTAACTTTTGTGGCCTCCTTATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATGAAGAAGTTATCTTTAATTTCTAAATTTTTATTATTTAAATTAATTGAAATATGTGAAATTTTTGAAAAATCAAATGCTCAACCGTCTATTTCTTTTACTGAACCAGGGATAGTAATAGATTCAATGCCTGCATCTCAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTCAAAGGTATATGCGACCATAAAACGCTCTTTCGCCAATTTCTGTAATATTTTTGCCTTGATATTCCCTAGGAATTGTTACTCTAGAATCATTTCCAATATATTTTGTAATTTTTCAAGTATTTGATTTAGTTAGTTCAAATTTAAAATTTTTCCTAAAATTTAATAAATCTTCTTCTTTGGTAGGATTTTCTGGTTTTGGTTCGGGTTTTGGTTCGGGTTTTGGCTCTGGTTTAACTTCAGGTGTTGTTCCTGGTTTTTCACCTTCTGGTTTCTTTGGATCCTCTGGTTTAACTTCAGGAGTTGTGCCAGGTTTTTCACCTTCTGGTTTCTTTGGATCCTCTGGTTTAACTTCTGGTTTGTTTGGCTCCTTGCATCCAGCAGCAACTAATGGCGCTGAAATAACCAAGGGAAGTATCCCCCCCATGGTCAACAAAATTTTTTTTGATTTCTTCATATTCTATATTTCCTTTTTTTGATTTTAAAATAATTTTATTTTTATATATATTAAAAAGTAAAACCTCTTAATAATAATAATAATAAAAAAAAAAAAAAAAAAAAAACAGGTTTTTAATATAATTGGTTGAAAAAATGGCTTATTTTCTATATTTTTTATGCAATTTATATATTCTTAAGGTCAAAAAAAATATATAAAAAAACTCAGATTGAACTGAATTTTTTTATTTTTTAAATGGCAATAAAGACAATTAATTGCATTTTTTTAAAACAGATTTCTTACATCATCTTAGTGTTTTAATATATCTGCAAGTTCATCGTTTGAATCTTCATCATTATCCATTGAAAAAAATGATTGTGTTTTAAATGTTCCTTCAGATACAAAGAATTCCTTTGTTTCGTCGTTAACATAATTTAAATCGTCTTTTTTTATTACATTGATTACTTCGTCTTGAATTTGATCAATGTCTTTGGGAGCGTCTTTTCTTAATCCCGTGGCGATTACCGAGACAAATAGCTCACCTAATTTGTTGCTTTCTTCAGATTCTAGAGTTGTTATACCAAAAATAATGTTAATTTCATTACCAACAATTTCCCTCATAGCTCCCAAAGCACTTTGAATTTCATTTAAAGTTACTTTTTGAGAAGCACTGAAATTTACTATTGCATCGCTAGCCCCAACAACACTTGATTCTAAAATTGGGCTTGTTATTGCAGATGTGATTGCTTTTACAGCCCGATCTTGGCCATTTGCTTGGCCAATGCCGATTACTGTTTCACCTTTATTTTTAATAATGGTTGATAAATCTGCAAAATCAAGATTAATAATGCTTGGGGTGGCAATAACATCAACTATTGTTCTAATAGTTTGCTTTAAGACATTATTTGCGCAAATAAATGCATCTGAAAAAGAAATATTTCCATATTGTTGTAATAGTTTATTGTTTGAAATAACAATATATGAATCAACACATTTTTTAATTTCTTGTAAGCCTTGCTTTGCAATTCTCATTCTCTTTGGACCTTCAAAGTCAAACGGAGTAGTTACAACTGCAACTACTAAAGCATTGCATTCCTTGGCTATTTGTGCAATCACGGGCGCTGCACCTGTGCCGGTTCCACCACCCATTCCTGCAGTTATAATTACTAGATCAGCTCCTTGCAATCTACTTTTGATTTCTTCACGCGAAGATTCAGCAGCAGTTTTGCCAATTTCAGGGTTAGCACCCGCGCCAATTCCACGTTCATCACCTAAATGTAAAACGCATTCTTGAGGAAATTTTGCTAATACTTGTTTATCGGTATTGGCAGCAATAATTTGAAAACTATCTAAATGGCTATTTATCATAGTTTCAACACTATTATTTCCGCCACCACCTACTCCAATAACAATTATTTTTGCCACCGGGTTATAATTATTTAATTCTTTTGCCATAGAACTCCTAATTTATATCGTTTAGCATTAAATTGTAATACTTTTTGGAATTACTATAAACTTCAATTGTAAATAATTCCCTACTAATTTCATTATTTAAAGCATTTTGTTTGATTTGACATTCTAAAATATTATCTATTGCAAGCATTTCGCAACTTAAATATTCATTTTTGATTTCATATTCAAAGTCACTAAATAAGTTAATTTTTTCATTTTGAATTAATATATTATCAAAAAGTTTTAATTCGCTGTTGAAATATAATTTGCAAACCTTTGAATCAATTTTATAATTATTAACAAAAGTTGCAACGCTAGTTAATAATTTATTTATAAATTCATTTAATATATTCTTTATTATTGGAGCAAAATTGCATTTGTAGGTTAAACAATTTACAACTTCACTAAATCAATTGCTTTCTGTATTTAATAAATTGCATTGTAACATTCTTGCTTTTAATTTGTTTAGTCCTAAATCTAATACTTGGCTTACTATATTTGATTTATTAATGTTTAAAATAAATTTAGCTTTATCATCTAATACATCAACAAAAATAGCTACTTTTTGTTTTGAATTTTTAAACAAAACTGAATTTGTCTTTAAACTATCTAAGCAAAATATTTTGGCAATTTTTAAATCGTTGCTACTAAAAATTTCTTGCAATCTTAGACTATTAGCATTTGATATTGTTTTTAAAGAATAAATTTGCTTAATATTAGTTTTTTTAACTAGTTTAAATTCCAATATTTTTTGGCCTATATTATTGAAGTTTGATCTAATATTTTTTAAATTACTAATATTAAAATTGTCGTTATTTAAGGTTAATAGTTTACTATTTATATTTCATTTGCTATCTTCAGACAACATAACAATAACTTCATTTATGCTTGCTGATATTAATTTTTGAATTTCATTCTTTGTTTGCTTTATAAAATTTTTTATATCGCTTTCAGTTAAATAAATATTATTAATTGCTTTTTTATAAATGCAAAAAGATAAAGTATTAGTTTTTTCCATAACTTCTACTTCGATACTATTTTTTAAAAATTTATATGCAGCAATAATAATTTTTTTCATTTAACTAATCCATTAATTTTGTAATAACGCGTAATTTTGCGCTTCTTGATCTTTTGTTGATTTCTTCTTCTTTACTACTTGGAAAAATTACTTTTTGATGTCACTTATGAGTTTCAACAATTAGTATTTTTTTTAACTTAGGGTCAAAATAATTTAAATTTTGATAAAACTTTTTAACTATAACGTCTTCTTTACTATGAAATGTTATAATCGCTATTTTTCCGTCTTTTTTTAATAAATTAGGAAGTTGGCTTAGCATTTCACTTAAGGCATTTAGCTCATCATTCACTGCTATTCTAAAAGCTTGAAAAACTGCTTTAGAAGGATTTTTTTGTCTTACTATTTTAGCTGGCAATGAATCCTTTATTATTTGATTTAACGTAAATGAATCAGATATTGGTCGATTTTTTACAATAGCTTTCGCAATTAAATTACTTAATTTTACATCAGCATTAGATTGCAATATATCTATAATTTCTTTTTCAGTTGCATTATTAATAATTTCTTTTGCAGTTAGAGTATTATCTAAATCCATTCTCATATCTAAATCAGAATTGATTGAATAAGAAAATCCTCTATTTGGATCATCTAATTGAGGACTAGAAACTCCTAAATCTGCTAATATTCCATCGACTTTATCAATATTTAGTTTTTTTAGTTCACAAGTAATGTTTCTAAAATCACTTTTTATTAAAATAAAGTTTTGGCTTATTTTTATTAATTTTTCTCTACTTTCGTTTATAGCTTGAATATCTTTATCAAAACAAATTAATTTTCCAGTTGTTAGTCTTTTTAAAATTTCTTGGCTATGACCACCACGGCCTAATGTTAAATCAACATATATTCCGTCTGGTTTTATTGATAATTGTTCTATTACTTCATTTAATAGAACTGGAATATGTTCCATTTAGAACTTAACTCCTGCTTCAAATAATTTTTGAGCCAAGTCATTTACATTATCATCATTTTCATAGTAGCTTTCTTGTTCATCATAAACTTCTTTAGCAAATAATTCACAAGTATCACCAGTTCCTAGGAAAACAACTTCTTTGTCGATAGCGGCTTTATTTAAAAGATGTTTGGGAATCATGATTCTGTTATTTTTATCAGGAGATACAACTTCAGTATTTCCAAAAATGTAGCGAATAAAATCTCTTACGTTTTTGTTAAGTGTGTTTCCTTCTTCCAATTTAGTTTTCATTTTTTGAAACTCAGAAACCGTTCTTAATACTAAGTTTTTATCTATTCCAATTGTGATATAGAATTCCTTGCCTAATTCTTGTAGTAATTTAGGCGGAACAACAACTCTATTTTTATCATCAATTTGGCGATAATATTTACCAAACATTCCCACATCCTCCCACTATCAACTTAATTCTAACATATAAAACAAGTATTTTTTAATTTTTTATAAATAAAAATAAAAAAGGGGGCAAAAAAGCATTAGCCCTCTTGCAATAGAACCATAGTAATTATTTTTTTAATTGTTTTATTAAAAAATAAATAACTGTATAAGTTTTTTGTTAAAATTAAAATATGCTAGTTTAGCTCAGTTGGTAGAGCAATTGATTCGTAATCAATGGGTCGGGGGTTCGATCCCTCTAACTAGCACCAAATGCACAAAGTAAGCGCCAAAAATAAAAAATAAAAAATTTATTAAAAAATTAATTTTTTTTAGTTATAATAATTAAGCATTTATTAAGTGCCGACTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTTGTCGTTGGTTCAATCCCAATAGTCGGCGCCATTATGGCCCTATGGTGAAGCGGTTAACACATATGGTTTTCATCCATACATTCGTGGGTTCAAGTCCCGCTAGGGTCACCATTGGAGAATTAGCTCAGCTGGGAGAGCATTGCCCTTACAAGGCAAGGGTCGTGGGTTCGAGCCCCTCATTCTCCACCATTTGTTTAAATACACCGCAAAAGGCCAATCGGCCTTTTTATTTTTCATTTAGATTTAAATTAAATGTAATTTTTATATAAAAAACTCGTAACCTTTTTTAATTACGAGTTTTTTATATTTATTTTTAATCATCAAATTGTGAGTAATATAACCTTGCATATTTTTTATTTAATTCTAGCAATTGCTTATGGGTTCCTTGTTCAATAATACGACCATCATCCACGACTAAGATTAAATCAGCATTTTTAATCGTACTTAGACGGTGAGCAATAACTATTGATGTTTTACCAACCATGATGTTTTCTTTTAATGCTTGTTGAATTATTTTTTCTGTATTAGAATCCACGTTTGATGTTGCTTCATCTAGTATTAAGATTTTTTTGTTTCCTAATATTGCTCTAGCTATAGAAAGAAGTTGTTTTTCTCCCTTAGATAATATTGAAGTGCTATCATCAATTATAGTATCATATCCATGTTCTAATTTTTCAATAATATGATGAGCAGATGCTAATTTTGCTGCCCTTATTACATCATCTTTGGTTGCTTTTGGATTTCCTACTAAAAGGTTATTTAAAATACTTTCATTAAACATAAAGCTATCTTGTAAAACAACTGCCGTAAAATCTCTTAAACTGTCTTTTGAAATTTCTCTTAACTCTATTCCATCTATCTTTATTGAACCTTTTTCATAATCATAAAAACGATTCAATAAGTTTATAATAGTAGTTTTACCTGCTCCAGTTTCTCCAACTAAAGCAATAGTTTGTCCTGCTTTTGCATTAAATGAAGCATCTGTTAGTTGATATTTAGGCGAATTTTCATTATATCTAAAATATACATGATCAAATGATATATTTCCAACGGGATTTTCTATCACAATTCCCTTTGAGTGGTTTTTTATATGTGTGTTTGGTAAACTCAAAAGTGTATTAACCCTAACGGCAGATGCAATACCAATTCTTGCATAAACTAATATAGTAAGCATTGATTGAAGTGTTGATAAAAAACTATATATAAGTGTTAAATAACTTATTAAAAATCCATGTTCTGCATTTGTATTGAAAAGCCCATAAACGTGAATATTATTTCCTTTTAATACAAGTGTTACAGTAACAATTAATATAACCAAAATATTGTTAGTAAAAACTATTCAAGGGTTAATTATTTGGTTATTAAGATCTCCAGTGAAGGCTGTGTTAAATATGTTTCTTGCTATTTGTTTACCATGATTATTAACTTGTTCTTGTTGTTCAAAAGTTTTAATAATTTTAGAATTAGTTAGCATTTCTTCAACAAAAGCGTTTAATTCCCCAAAATGTTTTCTAGTTTGAATAAATGCTTTTTGAGCTTTTTTAGTAATTAATATTCCTATAAATGAAAATAAGAATAAAACAGGTAAAACAACAGCAGATAAAACTAAGCTAACCATTGATAAAAATATTACAGATAAAATAACATTTATAGCACATTGGATTAATTCATTTAATAGTTTAAATATAGTTTCAGATAAACTATTAATATCATTTACTAAAATAGAAATAATATTACCTGATTTTTGATTATCAAAATACGAAACAGGAATATATAAAAGGTTTTTCATGGCGGTTTTTCTTATATTCTCGCAAACTGAAAAAGTTATTTTTACAAAGAATACTCCTGAAACATATCTAAAAATTCCATACAATATAAACGATATAACCAAACATGAAGCCATGCCTATAAATAATTTTAAATTAAAAGGTGTTTTATGATCCAATACATCTTTTGTTAAACATATAGTAAAAATATATCCAATTAAAAATGAACCTATTGCATAAAAAATTGCATGAAAAAATGAAAACAAAACAGCCAATCAAAATTTTGTTTTTGATTCACCTGAATATTTAAAAACTAATTTAAACAAAGTAATAAATGAAAGTTTTTGCTTTTCTTTAGTTCTTTTTTTCATCATTGCCTCCTTTTTTATTGGCTTAATTGGTTATTATATGATTCATTATAATATGAACATGATTTCATTAATTCTTCATGATTTCCTTTATCCAACATGTTTCCATCAGATAAAACAATAATTTCATCAGCATTCTTTATTAATGGCAATTTTTGGCTAATTATTATAGTTGAACATGAATATTTATTTTTTATATTTTGTAAAACTTGGTGTGCAGTAATATTATCTAAAGCACTAGTTGAGTTATCTAATATTAAAATTTTTGGTTTAGTTAATAAAGTTCTTGCAATTGAAAGACGTTGTTTTTGACCACCCGAAAGGTTTGTTGCCCCTTGAGTAATGACATGATCTAAATTTTCTTCAAATTTATAAACAAAATCATATGCACAAGCATCGCGTAATGCTTGATTTATTTCTTCATCTGTTGCATTATTCTTAGCAAAAAGCATATTTTCTTTTATTGTTCCTGTATTTAATAGGGCATCTTGGTAAACAATTCCGATCGTTTCATGTAAATTTTTTGTATTAACTTCTTTAATTTCATTATCCCCAATTAAAATCGATCCTTCATTATATAGATAGTTATTAACCAATAAATTTGCAAGTATGCTCTTGCCACTACCAGTAGGGCCAACCAATCCAAGAGTTTTTCCAAACGGTAGGCTAAAACTTATATTGTGCAAAGTATAATTTGGATTTGAAGAATAATATTTAAAACTTAAATTTTCAACTTTTAAATCAAAGTTTTTTGTTATTTTTTTACCATCTGATACATAAATTTTATCTTCGTTAGTATTTAATACTTCTATAACTCTAGTAGCAGAAATTTTTCCTTTTACAAATGCAGAAATAAATACTGCTCCCAATATAACACCAAACGTTACATAAAATGAATAGTCAATAAAAATATTAAATCTTACAATTGTATCATATGTTGCTACATTAGAAATAACATCATGAACAACTAATGAATAAAGCACCACAACAAATAAATTAAATATTCCAAAGAATAAAGGAGCAATACCTACAAAAATGCTACCAGCATTAACATTAAAATTCTTTCATGTTTTATTTATTTTTGCAAACTTTTCTTCTCTATTTTTATATAAATCATATATTTTTATCAAACGAGATCCCAATATATTTTCATCAACTTGCTTAGTTATTTCTTCAACAGCTAATTGAGCTTTTTTAATTGAAGGACTTGCTTTTTTTGCCATAATAGAAACTATTGTTAAAATTATTGGAACTAACAAAATTAATCCTATTGCCATTTTTACGTTATTCATGACGGCTAAAATACATCCACCAATTATCATTGAAATAGCCCTTGTTAAGACATTTGTAGCACTTATTAAAAATTCTCAGAAAACCGCAATATCATCACTCACCCTAGTAATTAATGATTCGGGTTTCAAATCAGATATATTTTTCAAACTTAATGTATTTAATTTTCTAATAAAGTTTTCTCTAATATATCTACTACTTCTTTCTGCTGCATATACAATTAAGCTAGTGCAGCCAAATGTTAAAGACATTGAAATTACAGTTAAAACTATTATAGAATAAATCATATAATTACGAGCATTAATAGCATTATTAAATGTAAAGTTTATTAATCCATTAAAAAAGCTTAAATCTACTTGGCTATTTTTATTTTCAATAAAAAGCAATCTAATGAATTGAGAAATTAATGTAGGAGTAATCAATTCAAATATAACTGAAATTAAAGAAATAAAGCTTCCTACAATAAACAATATTTTAATTTTTCATGGTAAAAATTTAATAAGCTTCAACATAATTTCTCCTTCTTTTAAAAGTTTTTTATTGCCACTTTCAAGGCTAATTAATAAATTATAATGAAACATTAAAAAAAGTAATAATTTAGTGCAAAAAAACACGAAAAAAAAGCGATTTTTCGCTTTTTTGTTTAATTTATTTTTTTAAACTAATTTTATTTTTAAATTCAACTTCTATTCCGTTCCATTTAGCAGTAATTGTATATTTTATATATTCCTTCTTATTTTTTTTGGTTGTATATTTTTCAACTAAGATTTTTTCGAATGTTAATCCTTCAGGTAATTTGTATTCAAATTTAGAAATATCATCTTTAATTGCTTCATCAACTGTTTTTTTATCGCCAATGTATTTTACGGTTACTGATTTTCTAAAATGTTCTTTTAAAACTATTTTTTGCATGAAGTTTTTATCTTCGTTTACCACTTTATTAACATTGACGCGTTTAGATGACATATTGCTAAAATCATTATTTTGAAAATCTTGAAGAGAAACTACAGAAACTCTAATTGATTTTACTTTGTTATTTTCTATTTCAGGAAATGAAAAGATATAAACATTTTGTACATCAACCATATTTTTTATATACTGAAACGCAATCATTGAATGTTTTAATTTTAATGTTGTTTTATCATAATCAAAATTTTGAACCGAAATGTAATTATCACCTTTTTCAATCATTTCTTTTTCAATGTCTTTGCTTAAAATATCAACTATATCAATTGGGTTCAAATTAAAAATTGTTGAAGTATTAATAATTTCTTCTACTCCATAGAAGTTTGAAGCTAATTCTTTATTTAACTTATCTATATTATTATTAAGTTCATCGATTTCTTTATTTAATTTATCAATTTTAGGTTGAAGTTCTTTTTGCTTTTGGTCATATTTTGTTTGTGCTTCATTAACTTTAACTTCAATTTCATTTTTTTTGCTTTGTAATTCGCTAATTTTTTGTTGATGAAGTTGCTTTTGTTCTTGGCTCATTGAAGAAGTATCTTCATTTTGCAATTTCTTTAATTGATCATCAACTTCTTCCAATGAATCTTTTAATTCACTAATAGATGATTTTAATTCGTCTAATTCACCATTAATTTGAGTTAATTGAGCTTGCTTTTCTGATAAACTTTGATTTGCTGATGTTTTTTCTGGATCTAATGATTCTCATGTAGCCTTTTGTTCTTTGAAATATTTTAAGAATGCATCTTTATTTAATTCTTTTACGTCTTGGTCTTCAATAGTTGATGTTGGGGCTTGTTGGTCATCTATTTCAGTTTCATTATTTTGTTTTCTAATCTTGTTATATTCATCAACGCCCAATTTGCTTAAATCAATTTCAACAAATTTTGATTTCTTAGTACTATTTTCATAATCTTTAAATGAAACAGTAAGATTAATCAAACACTTATCGCCTTCAAATTTTAATTCACCTAATGTGTAATTAATTTCACTGTTTTTATTATTAATTCAGTATGATTTATCTTCTAATTTGTCAAAAGATTTCTTTGAAATGTAATATTTGTCTTTACTATAAAATGGATAAATTGAAGTTTCTAAAGCTTTGTTTAAAAGTTCATCTTGTTTTTCTGGTGATAACAATGCAATATTATCTAAACCATTTGCAAATTTATCTAATTTAACTTCAATAGTTTTAGATTCACTTATTTCTTTATTTAAAAGACTTGACAAAGTTAAAGTCAATGTTATTTTTTTATCTTCAACTTTTAAATTGCTTAATGCTACTGAATATGAAGCTTGGTCGTAGCCTTGAACTGCAAAATTTGGTTTTTTATCAAAATTGTTTATTTGTTTATTTTTTAAAATATCTTGATATGACTTGGCATTTGCAACTAAAACATTTGTTTTTTCAATTTGATCTTTTAGTTCTTTTTTGGCCTTATTTATTTTTTCTTGCAATTCTGGAGTTAGTTTAAAACCTTTTATTTCATAAATGTATTTTTGACTTGAAATTTGTTTATTCTTGTATGATAAATAGTAAAGAATTGACAAGTCATATGTTCCATCTTCTCCTGTATTTTTAGTAATTTTTAACAAATCTTTAACAATTGAAAAGTTTTCTTTAATAGTTTTTAAAAATACTTTTGATGTGTCTGCGTCTTTTATGTATGTTTCTTGTTTGTTAGGATAGTCAAAGACTAGATTTTTTGAAACTTCTTTAAAATCTTCTTCTGAAACTTGATCTAATCCAGAATCTAAGTTTCCCAAATTGCTTTCATAAACCTTGTTTGAAATAATATGTTTACCATTTTGTTTAAAAATTGCAACCTTATATTTCAAAATAAAGTTTCCTTTTTCATCTTTTGTTAATAACAATTTAGAGTTTAACTTGTCTCCACTTCTAAGAGGTTTTTCAGGATTTACCAATTGAACAGATGCGCCTGATTTAGAATTTCATTTAGCAATAGCAAATTCTTGATTTTTAAAATCATATGCTCCTTCAAAAGCCATGATATTTTTGTTATTTCTTTCAAAAAGAATTTTGTATTTGTCTAGATTATTTAAAACCTTTTTAATAAATTCTAGATTTTCAGCATTTCATGTTATTTTTGAAATTGATTCTTCACCATATTTATCAGCATCATTTTTATTTGTTTCTTCTTCATGACTTGGATCTTCTGGTTTAACTTCAGGTGTTGTTCCTGGTTTATCTTCCGGTTTAACTTCAGGTGTTGTTCCTGGTTTTTCACCTTCTGGTTTAACTTCTGGTTTTTTTGTTTTTACGCAACCTGCTGCCACTAATGGTATTGCAAAAACCGAGGTTGCAACACTACCTAAGGCTAATAAAATTTTTGTATGTTTTTTCATTTTTTATATTTCTTTTCCCTTTTTCTTAAATTAATAATTTAAAAATTTATCACACGAATAAAGGCTAAAGTTCAAATGACTGGTTTTTTCTTTAAAAAAGAAAAATAAATAACCGTTTGGTTATTTTTTATTTAAAGATAATGTTTTCAAAAGAAACAAAAAAATAATAAATTACAAAAATTGTTACGTTATTTTTTGATTTAGCAAATATTATAATGTGTTTTTTTAGCCTATAAAAATATTTAAAAAACTAGCATTTTAAGCTAGTTTTTTAATATGGTACATCCTGTGGGGCTCGAACCCACGACCCAGGGATTAAGAGTCCCTTGCTCTGCCAACTGAGCTAAGGATGCGTGTTTTTGCGTTATTTATTATAACATAACTAAAACATTTTTTTAAAATTAAACAAATAAAAAGTAGCGTTTGCTACTTTTTTTGTTACAACTTGATGATATGGTACGCGAGGAGGGACTTGAACCCTCACGCCGCAAAGCATTAGTGCCTAAAACTAACGTGTCTGCCAATTTCACCACTCGCGCATATGGTGCCGTTTATAGGACTTGAACCTACGACCTACTGATTACAAGTCAGTTGCTCTGCCAACTGAGCTAAAACGGCTTTTGTAAATGCTTTATTATTATATAAGGAAATTTTAAAAAATTTTACATTTTTATTCAAATTTAAAATTTTAATTATTCATAATATCTTAAAATAAATATTGAAATATTATTTTTTACTATTACTATATGATTTTTTTATTAACTTTAATTTTTAAAGATAATAAATTAATATTTTTAATGTATGATTACATGGCAAGGACAAAATTATGTTTTATAAGTTATATAAAAAAAGAGGCCAATCAAGTTTTTTTGCAATAAAAAAATTTGCAAAAAATAATAATATCAATAAAATAGGACATTGTGGAACATTAGATCCTGAGGCTGAAGGTCTTTTAATCGTAGCAACAGATGAAGATACAAAGGCCTTGTCTTTAATTGAAAATCAAAACAAGGAATACTATGTGGAAGCAAAATTCCATTACTCTTCAGATAGTTTTGATTTAGGAACATATGTAACTAAAATACAAAATTTTCAAATAATAACTAAATCTCAAATTATACAAGCACTAGAAATATTAAAAAAACAAACAAAACAAGTGCCTCCAATTTATAGTGCAAAAAAAATTAATGGAACAAGAGCATACAAACTTGCTAGAGATAATAAAGAATTTAGTTTAAAAGCCAATGATATAAAAATAAATAATTTAGAATTAATCGAATTTAATGAAGTTAATCAATCCATCATCATTAAGGCCAATGTTTCAAAAGGCACATATATTAGATCATTGATTCATGATTTAGCTAATATATGTAACACAGATGCAGTAGTAGTTGTTTTAAAGCGAATTAAAATTGGCAATATATGTTTAAATGGTCAAGAAGAATTTGAAAAAATTACAAACTTAAACGAACTTTTTAATGTAAAATTATTTAAGCTAACCAAAAATGATATTCAAATATTAGCAAACCAAAATTTAAATAGTTGCTACTTTGAATCATTGAAAAATTTTTGCGGAAACATTATTTTTATTTACTTGAATGACATAGTAGGATGAGGAAAATCAGAATTTGGAAATATAATTATTTTCAAACGATTTTTTAAACGCCTTACGATGTTAATTTCTAGCTAGGAGTAAACATGACAAAATACAAAATGATAGTATTTGATATTGATGGAACATTATTGCCATTTGGTGTTACTCAATTAACACCAAGAATGCAAGAAATGTTTGAGAAATTAAAAGAACAAGGCTATGTAAATGTGTTGTGTTCTGGTCGTGATATTTTTACAGTAGGCGCCCAAATTGATAACCCTTATGTTGATTATTTTGTAGGTGCAAATGGAACTTTTATTCTTGATCTAAGAACAAAAGAATATATTTTTGAAAAAACTATTAATTACAAAGATTTTATTGAATTTAGAAAATATGCAAGCGAACATAAATTATCTTTTTCTTTTGTTGGAAACAAATGGGGATATTACAATGAATTATTTAATATAGATCATTGATTTTATAGACCATATAAAGACAAATTTATCCCAGAAAAAGAATTCGAATCGCAACATGATAAAAATTATTTAATTACTATTTCAACTCCAACACCATCTATAGTAGCAGCTGATTTAGAAAAATTCTTCAAAGAAAAAAATCTTGATTTATGAATTCTGGCTCAATGAAATGGTGGCATATTCATCTCTGCTAAAGGAATAACTAAGGCTGTAGGTTTAAAAATACTAGGAGATTTATTAAATATTTCTCTTGATGAAATGGTGGCATTTGGTGATTCAGAAAATGATATTGAAATGCTTGAAAAAGTGGGATTAGGTATTGCTATGGGCAACGGAGAAGATGTTACTAAAGACGCTGCAAAAGAAATTTGCTTGCCAGTTACTGAAGACGGCCCATATTGAAAATTAAGAGATAAAGGATTTTTTGACTAATAAATTTCAACCTTATGGTTGATTTTTATTTATTTTATTTTAAAATACTTATATTAGGAAAAAAAACACATGGCAATTACAAGTACACAAGATCCAAAAATTTTGAAATATAAAAAATTACTTACCCCCAAAGGCAGACAAGAACAACAATTGTTTATTGTTGAAGGGTATCATTTAGTAAAAGAAGCAATCGAAGCAAATATAGTTATTGACATTTTAGAATTCAATGACATACTAACTTATAAAAACTCAACTCAAGTAACTAGAGCAATTATTAAAACATTATCAACAACGCAAACACCTCAAAATATCATTGCAATTTGTAAAATACAAAATTTAAATGTTAAAGCAAACAAAATAATAGCCCTTAATAAATTACAAGATCCCGGAAATATTGGTACTATTTTTCGACTTGCTAAATCTTTTGGCTTTGATAGTGTTATAGTTGAAAATATAGATATATACAATGAAAAGATAATTCGTTCATCACAAGGAGCAATGTTTTCACTAAACATTATTAAGACAAATGATTTAAGCAAAGAACTGTCAGCCTTTAAAAAAGATGATTTTTTAGTTTATAGTACAACATTAAATAAAAATTCAATTAAGCTCAATAACATTGATTTTAGAAATAAAAAAATTGTTATTGTTTTAGGAAATGAAGGAAATGGTATAGACAAAAAAATTATTGACTTAAGTGATTTTAATGTTTACGTTCCTATTGAATTTGAAAGTCTCAATGTTGCTTGCTGTGGCGCAATTATTATGAATAAAATTTATAATGGAGATAAATAATGCAAGTTTTTGATTGAGATTTTAAAGCAAAGTTAAACATAAATGAACCTTTAACAATTTGTTTAGGTTCGTTTGAAACATTACATTTAGGGCACAACGAATTATTTAAAATAGCAAAAGATTTAAAGCAAAGTTATCCAGATCAAAAATTAGCAATAATGATGTTTAAAAATCCAGTTAAAAATTCTCAGGTATTAACAAAAAAAGCAATGCAACCAAAAACCAGATTATATACATTAGCAATGTTGGGATTTGATTATGTTTTTTTAGTTGATGTAAACCAAACGATATTGAATTGTAGTTATAGCGATTTTATTCAAGGATTAAAGATAAATAATGTTAAAAATGTTGTTTGTGGTCAAGATTTTAGGTTTGGGTTCAATAGAGAGGGTAACATTGATAAATTAAAAAAATATTTCAATGTATATGTTGCCAATGAATTAAAGGTAAATAAACAAAAAATTTCTTCAACAATAATCAATGAATTTATTGAAGAAGGAAACATAAATGGCTTGAACAAATTACTAATAGGCAAATATTCAATAATTGTAAATTCTCAAAAATTTAATTTTAAATATCCAGAACATTTAAATCAATTAAAAGCAGGAATTTATATTGCCAATGTCGTTTGAAATAACATTGAATATCACGGCTTAATTTTTATAAATAAAAATCAATTTGACTCAAATAATAACCTACAAGAAAACAACATAATTTATTTATTTGATATTGATTATTTTATTTCGAAATATCAAGAACTTTACATTGAATTTTTGCAATGCATTAGACATATAAATAATAATAGTGAAAATCAAATTTCAACAAATGATATTGAAATTGCAAAGAATTACTTCATTGCCAACAAAAAATAATGTTAAACAAAAATAGCAAGTTTGTAACACTTGCTATTTTTTAACAAAATATATTATTTTAATTTAATCTTTGTTGTTCAAGTCAATATATTCAATATCACCGTTTGAAATAACTTTAATTAACCCTAAATTATTTAAATATCCATATAGATCATTTAATTTTGAAATATTATTTTCTGTTCTTGCTATTAGTAATGTTTTTAAAATAGTATTAGTTAAATTGTTTTTTAAAATAGAACTTTTTGAATTGTTTAAAACTTGTTTTATTAAATCAAACATTTCCAACGAACTTATTTCTGATATTTTTCTATTGTCACAATCCTTTGGCATTCTAAAAGTATTTGGCTTTGAAACATCATGATAAAAGATTTCACCATCAGGATTTATTAATTTTTCTAACATTTTATTATTAACAAAATAATTTATAGAATTTTCACAAAGAAATTTATAAGTATATTCGTCTTTATTTAAAGGTAGGTTCACGAATATATCTTTTATCATTTTATTGATTTCTTTTTCTCTATAAGTATGGCCATAATTAAACATTTGTTTAACAATTTCATAGCCATTTAACAAAGGATTTCTTTTGTTTGACTTTTCTATAAAATTTAGTGTCTTACGATATGAGTAATTAAACGGAAGTCATTCTTCAAAATTAAAATTATCTCTTTTTTCAATAATTTCAAATTGACTATTTTCAATTGTTTTATATTCATTTTGCTTTACAACAAATTTTTTTTGTTTAATTTGATTTACTTTATCAACTATTTTTTCTAATTCTCTTCTTTTGTTTTTGAATCAATTTAAAGATCAAATTCTGTGAATTATTCAACCTCTTGACTCAAGTAGCATTTGTCTAATCGCATCTCTATCACGAGCGCTTTTTGATGAATGATATGTGGCGCCATCGCATTCAATACCTAAAATATATTTCGATGGATTTTTATCATCAACAATTGCTAAATCTATTTTGAATTTTGAACATCCTACTTGAGTATTTACTTTATATCCAAGCTTCTTAAGTTCATCATAAACTTGGATTTCAAACTCACTATCAAATGTTTTAGTGTCATCTTGTAATATATCATCTGATTTGCTGTCGTAACCAAATTCTGCAAATTGTAAATATTGCTTTAAAAATCTTAGTCCTTCATTTTTTAAATCGTCTACTTTTAAATCAGAATATGATATTGAAGATACAACAATAGTTGCTTCTTTGGCTCTACTTATTGCGACGTTTAGTCTCTTTTCACCGCCAACATTATTTAATGGTCCAAAGAATCTTGACAATCTTCCGTTTTTATCCCAACTATTTGTAATTCCTAAAATAATAATGTCTCTTTCATCACCTTGAACACTTTCAATGTTCTTAACAAAAATTTTATTTTCTGTGTCTAAATCCATATATTCAGGATTTTCATTCATAAATAAGAATAATTTGTGATCAATTAAGTTAGCTTGTTTTAAATTAAACGTTACGACCCCAAATGTTTTTGTATTGCCATAATAATCTTTTAATTGCTTTATTAATTCAATGATTCTATTTGCTTCAATTTCGTTTGTTTGATCTTCGTAAATTCCATTGACTTTAATAAATGAAACACCTTCAAAAGGAAGCAATTCCCCGTCTTTCTTGGCGGTTTTTTGATATGTTGGAAAAGTAATTAATTTATTATCATAAACGAACTTATTTGAAGGCAAAATTAATTCTTCAAATTTTGAACGATAGTGTCATTTTAATGTAACTTGTCTAAATGAACTTTGCATTCTTTCAAGAAGTGAATCATAATCATTTTCTTCATTGACTATTTCTTCGTCTTCGTTTGGTTCTTGCTCGCTAGACATAGTTTTAAAGAAATTAGTTGGTGGCAATTGATTTATATCACCGGCAATAATAATTTGTTTAGCCTTAATCATTGATATCAAGGCATTTTCTGTCGTTAATTGAGAAGCCTCATCAAAAATTACACTATCAAATGTAATGCTACTATCCGCTAAATATGTACTTAGCGAAGAAGGAGACATCATAAAACAAGGCTTAATATTTTGAATTAAATCCGGAATTTCTTTAAATAGTTTTCTAATCGGTTTTTTATTTCTAACTTTTGAATATTCCTTCTTTAAAATTTCTAATTCATCATTGTATATTGTCGAATTTAACGTAGGCAAATTGTTTGATAAATTTTCAATTATTTTGAATTTAGCAATTTCATTTAGCTTTAAACAAGAGTCTTTAAAAACATTGATAATTTTTTCATATTCTCTATCGTTTCAATCTGCTAAATCAATTGATAAATAGTAATCAATTAAAAGTTGATAAAATCTTTTATTAAAAATATTTTCAATACTATTTTGTTTTAAACAATCAATATCTAGCAATTCAATAGCATCCACAAAATCATTAGTAAAGGGATTGTCTTTTAATTCTTGTCTTGATTTTATATAACTACAATATGCTTCAGGATATTCATCAGAATCAATTAAAAAATTTAGTTTTTTTATAAAATCATTAACTTTATAATATGAATTTGAATAATCTACATATTTATTATTAAAGTAGTCATTGAATTTATCTTCAAAAGTCATTAAATTTTTATATGAATTTATGTAATTATTTGTTAATTGAATATGGCTTAATAAAAAGTTAAAAAAGCCAATTTTTAAATTTTGATCAAATTTTAATTCACTTAATTGATTATTAATAATTTCGTATTTAGAAAATGTATCTAATTGTTGCTTTAAACTATTAAGATCCATTTCATTATAGTTTTCTTTAAATGAAAAAGGAATTTTATTTGTTAAAAGCGTAATTGCATTTTTAGCAAGTTGAATGTTATTTAATGCCTCTAAATCTTTTAACAAGTTTTTGTAATTTAAATTAGAAGATATAAAATTAATTTTTAATTCTTTTTTTATTGCTCTATATCTCTTTGAAAAAAGACGCTTAAAAATATTATTTCGAGATTTCACAAAATCTAAATTCTTATAAATTTCACTATTAAAAATATTTTGATTATATCTTGAAAGAATTCAGTCAATATCAGGTTGAATTTTTTCTTTTTGCAATAGTATTTCATTTAGGGTTTGTGTTGCATTAGTTTTGTTATATGAAAAAATTTCTAAATATTTTGGATTTATATCTATATCTTGTATATTTGAAAAAATTTTAAAAAATAAATTAAATAATGAATAATTGCTATTTTTAATATTCGGCTGATAATTGCTTGGAATTTCAAGGTCTAAGAAATTGATTAGGCTTTGAATTTCATTAATTAAATGGCTAGAAATCTCTTTAATGTTTTCTAAATCTTGAAGCTTTACATCCAAAATCGAAAGCCCGTATCATAAACTTTCCTTAGCGTTAAAATTTATATTTTTATAGTTTAAATTAAACTTATTTAAACTGTTTTGAATTTCAACAAACTGTGCATTATCCATTTTTTCAAAATCTTTAATATCAAAATTTATACCTTTTGTATTGGCAAGTAATTTTTGATAATCACCAATCATTAAATATAATGAGCGACCTAATGGTTTTCTATTTGATAATATCTTTCTAACAAAATCGTTCATTTTTTCTAATGAGTCGCTATATAAGTAATTATTATTAGTAATAACTTCGTCACTTACTTTTTTAAATTTTTGACCGTCTTGCAATGTTTCATACAAATCATTAGAAAAATCTTTTTTATTGCTGTTATTGCTATGAATAAGTAAAATAAAATTAGCAAATCCAAGATTTTGGATCGATTTATAAACAACATCCAAAGCAGCCTTTTTTTCAGCAACAAATAGAACCTTTTTATTTCTTGCAATTAATTCACTTATTATATTTGCTATAGTTTGAGACTTTCCAGTTCCTGGAGGACCTTGAATAATAAAACTGTTTCCTTTAATTGCTGATTGGATAGCTGCTTCTTGCGAAGAATTAGTCATTAGGCAGTGATAATAGCCTTTAGGAGAAACTATATTATCAACTTCTTCTTCAGAATACAAATATTCAGAACTAAAAACATTGTTAATCCCTGTCATTGCATTCATAATTTCTGAATTAATGATATTATCTTTGTTTTTATCAACATCTAAATAAATGTCCATAGAAGAGAACCTAAAAGCGGACAATGAAGCTTCATCAACTATTTTTCAATTTTGTTTTATAGAATCATTGAAGGAATTTATTTTTTGATTTATAAACTCAATGTATTGTGAATATCTATCATATGGAGAACCTTCAGCATTATCAAATGCTTCAAATTTAATATCAAGTCCAAAATCATCATTTAATTTCTTTATCAATGAATTATTTACAACAAAATCATTGCTTTGAATAATTATTTTTTTATTTTGCAATCCATTTTCGCTTTCAATTGAAACAGGTAAAAACAAAAGTGGGCTATAAACGTTTTTTTCTGTTTTATCCTTTCAATGAAGCCCACCAAAAGCCAAATATAGAATATTAATTCCTTGTTCTTCTAATAAAGTCTTTTGCTTTCTTTGCAATGACGACAATATTGCTAATAATTCTTTTTGAGAAGCAGAGGACAAAAATTCATTGCTTTCAACTTCGATGTCATCATCAGAATCTTGAGAACCAATAAATTTAAATTCACTTCTATTTTCAATTTTATCTAAAATTGATTTTATGTCTTCTTTTTCAATTGTTATAGTTGATGATTTATTTTTTTTATAATTTATCAATATATTTCTTAAAGTTGTGTCTAATAGCTTATTCTTTAAAGACTCATATGCCAAAGTTTTTGTAATTAATTCTTTATTTTCCATTTCTAAATTATATTATTTTAATTTTAAAATAAATGTTGTTTAATATAAGATTTTATTAGCATATTTTAAAAAACAAAAATTTTAACGCTTGTGAATTCAAAATTGAAATTTTTACATTTATTGATCGGCATAATAATAAATTCATTAAAAAAATGATTCAAAACATCCCAACAATGTGGGATGTTTGTAAAGACAATAAATTTATTATTCTAAATTCATTGTCTTAGTTTAATTAAACTTAATAATTTAATTTTTATTTTAAATTTAAATCCTTTGATATTTTACTATTCAGCAAAAACAGTATGTTTTGCAAATCATTGAATCATTCTTACTGGCATAAAGAACGCTCCATAAACGCCAAGAGTAACAAATGATAGTGCTCATCATTTTATAAATGAGCCAAACAATTGCAATCCGGTACCATTAAATTGTAATTGCTTTCCATTTACATAAGTATGGCGTGTCATGTATTTTCTGAAAAATACAACAATTCATGGCAATGTTAAAAATAATGTTGCCATGCTAAGCATTACTAGAAAGAAAACAAGCAATTTCATAAAGAAAAATCCCAAAACAGATCCTGTTCACTTTGAAGAATTTTCAATATTATTGTTTTCAAAATTATTCATAATATCTCCTTTTAAAAATTAATATATTTAATGTTATCAGATTTGATTTTAATATAAAAAAATCTTATTTTCTGCTAAAATTTAAACTTTTTTTATATTTATTTCAATAAAAATAACTAATATGTTTATAAAACGTGTCTATAATCTAATATTTTAAAAAAAATAAATTTTTTAATCAATAAATAAAAAAGCAAGACTCGTTTTATTTTGATGCGAGTCTTGCTTTTTAATTTTTAGTTGCTTTTTTCTATTATCGAACCCATTTCTAAAAAACCTTGAGCATCCAGTGCTGCTCCACCAACTAAAAAGCCATCCAAATCCTTAATTTCAAGCAGTTTTTCAATATTTTTTTGTGAAACTGAACCACCATACAATACTGGAACTTGATTATTAGTAATTTTTTTAATTACAGATATTGCTTTTTCGATATGGTTATTTTGAGGAATTTTTCCATTTCCAATGCAATAAGTAGGTTCGTATGAAATAATTAAACGATTATAAACATCAACATCTTCTAAAGCCTTTAATATTTGGTTTTTTATAATATCCTCAAAGACTTTATCGCTACAATTTTCTTGATCTTCCCCTATACACAAAATAGGAATAATGCTAGTTTTTTCAATTTGTTTAATTTTTAAATTAATCAATTGATCAGTTTCATTAAATAATTTTCTACGTTCACTATGTCCTATTAAAACAAAGTCAATGTTCAATTCAACTGCTGATCTAAATGAAACTTCCCCAGTGTATGCACCTTTTTCTTTATAAAAAATATTTTGAACTCCAAACAAGAAATTTCTTTTATTATATTGTAAAGAAAGAGACATTGCATCGAATGACAAAGCCGCTCCAACTATTACATTTTTAAAATTTTTAATTTTATTTAATGATTGCAGTTCATTTAAATAATTCTGACTTTCTAAATAAGATAGATTCATTTTTAAATTACCAATTAAATATTTCATATTAACCTCTTTTATTATCAAACTATTCTTAATGCCAAAAATGGAATCAATATAATTAATATAATTAATCCATATAAAATTAATAAAAGAAATGATAAATTATTAATTGATTGATAGTTTCTTTGCTTTTCAGCTTTGAGTCTTTCACGTTCAAATTCTTGACGTTCTTTAGCAATAATAATATCCTTTTGATGAATTGTCATTCCTGAATAAAATTTGTCTTTTGCGCGACGCATTATGATTCTATTTCTAATTAAATTAGCAATAGGATTGACAATTCATTTCCCCATTCCTTTTCTATATACAACAATTATAAAATTTATAGTAATTGCAAAAATCATTGATACAGTCAATGAATTTTGCCAAGAATGAACATATTGACCATCATCAGATTTTTTTGAATATCTAACTATAAAAATTAATGCAATATAAATCACTAATGAAACAAAAAAATAACTAATCGTTGCTTTATTTCAAGCATTCTTTCAATATGATATTGATTTCTTTTGATTTCTAAAATTTTTTTGTTTTAATGTCATAACATTATTTTATATTATTTTTTAACAATGCAAAACAAATTTAAAATATATCTGATTAATGATTTTAAAAATAAAAATTAAATATTATTTTGTTTCTTTGTATACAAGGTAAATTAATTTATTTGATTAGTGAATCTATTTTTTTGTGATAAATTTCGTTAAATTCTCTTTTATCATGCTCAACTTGTTCGAAATTTGTATATGAATTGTCTTTTTCGTTATTAACTAATTTATTAATATATGGGTTTATAAATTTTTCAATAAAATCTTGGCCATCTGGTTTTTGAAATATTTTATTTGAATTAATTTCTGCATCAGCCAAAGTTTCTACAAACTCTCAAATAGACTCGTTTAATGCTAACTTTTGAGTTATAGAATTAATTTGTTCATTTAAAGATTGTGTTAAATTATCATTTGCATGTCATTTGTTAATTTCTTCAATAATTTGTCCCTTAAAATATTGATATTGATTTATTAATGATATATATTTTTCTTTTAAATTGTTTTTTGCAATTTTATTGATAATATTTTCAATTACTTGGTCTTGGAATTTTTGTTGCAATGCCAAATTAATAAATTTAGTTTTTTGAATTTTAAGCGAGGTTATTTGCTTAATTTCTTGCAATTTATTTAAATAACTGGCAATATCTTTGCTATTAATATTATTCAATAATGCTTCTAGATTAATTGCCTTTAAAGTTAAAGATAAATTATCAGCAATTGATTTTAATTCGTTTCTTATTAATTTATCTATTTTAGAATTGTCTGCATCTAATTTAATTTTATTTTCAAGATTATTTAATTCGTTTACTAAATTATCAATTTTTGTTAAGTCTTTTAAAAGTGGTTGAATTTTAAGTATTTTTTGTTTAGCAACGTTTATTTTTTGTATTTTTTCATTTAAACTTTCTATTGAAGATTCAAGCGATTCTTTATTAAAAGATTCCAATAATTTCTTGGCATCCTGAACTTCTTTTAAGGTGTTAAATTGTTCAGTTTCTCTTGTTGAAAAGTTATCTTTTAAATAACTAAAAAGATCAGTTTTTGCATCATTAAATTTTTTTAATAATTCTTCAGTTTTTTGTGCATTTTCATCTTTAGTTTGTGTGGTTTCTATATTTTGTTTAGGTTGCTTAGGCTTTTTATAACAAGAAGCTGCAACTAATGGGGTTATTAAAATAGTTGAAGATAACGGCAATATATAAAGTAATTTTTTTCTCATTTTTCTCCTATGAAGTGTGTAATATTTTTTTAATTTTATATTCTTAAATTGTGCTATCTACTTGTATTTGTTATAAATCACGATTATTTTACCAAAATGTTCAATAAATTTAAGTAGTTTTATTAAAACAAATTATTTTTTTAAACCTAAATAATCAAAAAAATAGTCTAATTTAGGCTATTTCTTCAATTAAAAATTTTTCACTATTTTATATTAATCAATTTCAAGTGGTACTTCGCTATCACCATCACTACTAAAAATTCCATTATTTGATTTATCAGGGCATATTACACTATAAGAAATTACTGGCAAATCTCTTAATCTTCCCTCTGATTTTAGTTGTATTGAAATGGGGTGGCCAAACGGAATAAGTATCTTAGGCAATGTAACCTTAAAATATTTTACAAAATCTGCATCATTTATTACATTTTTATATGCTTTATAAACTTGCGAATTTATTCCACTAGTTTGTTTTTTTCTTATGTTGATTTCAATTTGTTCATCAATAGATTTATACTTATTGATTATTTCTTTTAAAATTCTTTGTAATTCTTTTGTTCCACGTGCAGGGTCATTTAATATTTCTTTACCTTCATTAGTTAAAGTAAAACCAAATTGGTCTCTAATATATGACAATTGGCTTTGGCCTAATTGACCTTTTGCTCTCTTATTAGGATCTACAATGTCGCCAGATTGAGATTGTCCGGTCGAATCTTCACCTGTGGTCGTAGAATCTTGACTATTATCTGTTGTTTTGCATCCTGCTGCAACTAATGGCATTGCAACAACTGAAGTTGCAACGGCACTTAGTATTAATAAGCGAATTTTATTTCTCATATTATTCCCCCTGAATATAAACATATCGTTCATCTTCAATAACTTGTAAAAATTGGTTACTTCTTAATTTACATATTATACTATATTTTATAAATATAGTATCTGATTTTAAATTTGTATCACCATATCCAAGTTCTAATTTCAATGTATGATTTAATAAGTATTTCGAATCAGGCAATGAAATTTTAAAATATTTTAAAAATAACTCATCACTGAATATGTTTGACTTTTGATCATCTTTTTTGTATTTATCATTGAGTTTATCAATGATTTTGAATAAAAATTTCTTATTCTCTTGATTAAGCGGTATTTTGGATTCAAAATTAAAACTATCATAAATTGCTGCAAGCATAGTACTAGACATGTTTTGATTATTATTAGATAAAACATTATATTTAGTATGACATGGGCAATTAATTCCTTCAATAATCTTGGTTTTTAATAAATCATTTTTGGTTTTTAAATTAGAGGATTTTTGGCATGAAACTGCTGCAATTGTTGGAATTATTAAAACTATTGGTAAACAAAATAGTGTAATCTTTTTATTCATTATTCTAAATCTAATTTTATTTCTTGATCTTTGGCATCATATGTTTTTACATCTTTGCAATATATGTCATAGATTAATGAAATATTGTTATTATTATCAATTTTAAAATTAATTATCAATTGGTGATTTACACCTAAATTCGGCTTAACCACTTTAAACAATTTTAAAAAGTTTGCATTATTAATTAACCTCAAACCTGCTAATTTATTATTTTCAGAAGGGCTAAGATTAATTTGCAATTCATAATCTTTACAAATCTTTTTTAGCTCATCTGCCAACGCCTTATTGCCATAATTATTTTTTAGTATTTTGCTCTGTTCAGTTAGTTCGAAAACAAAATCATTTTTAATTGCTTCTAATTGAGAAGAATTAAAAATTCCACTTTTATTAGTGTAAATTTCATTTGCTTTTGCTGTATTTTCATCAGTCGAAACACAAGATGCTGAAATTATTGGCGAAGCTAAAACTAGAACTGCCCCTATAGATAATATTATTTTTTTCATATGTTTTTCCTATTCTATTACAATATCTTGCAAGTAAGACAACCCGTCATAACTAATTGGTTTATTAATAAATTTTTGGTATATTACTTTTGAAAAACCAACAGTTTTAATGATATTTTCAAATGAAAAGGTATAAGAAAGTAAACTGTTAATATCATTAATTATATTTAATTGGTCTTGGTAAGAGATATTTTCTAAGTATTTTAATATAATTGATTTTATTATAGAAAAATTTTTGTTGTAATCTTCTAAATTTTTGTTAAAAATTTCATTATTTGTAATTAGTTTTGTTTTATTTAATTCATTTAATATTTTTTTGGTATTTTCTAGAATAAAACTATTGCTTTTAGTTAAGCTAGAAATTTTATTAAAGATTAAAAACAATTTATAGTTTTTAGCAATGGCTGTTGCTATAAATTCTTTAGTATTTGATTTTAAAAGTTCAAAGCTATTATTGCAATATATTGAAGCATCTTGATTAAAATACAAGTCATATTTTGCTAAATCATCAATAACTACAAAATTTGGCTTTAAATGTTTATTTACTGCTTTAAATATTGTTTTGATATGTTCCAAAACATTTTGCCTTTGGTTATTAAATTGCAAATTCAAAAAATTTAGTGGAATATCAAATTCAATATCAGCAGCATCTGGATGTTGTTCATAAAACTTTTTTATTATATCATTTAGTTGTTTAGATATTTCTTGAAAATAAGCTGATTTTAATTTGTAATCAAGATTGCTTGATTGAAGATCGTTTAATATTTTTTTGTTTGCAAGATAATAACTATTGTCAATAAAGTCAAGACTATTTTCATGAACACTTTTTGGAATCAATGGCTTAAAAATGTTTACCATAGAATCTTTTAAAACATGATAATTAATTCCACTATCAAAGCTTGAAATATCTAAATCGCTTGGCGCTTGAGATGTTCAAATATCACCTTGGTTAGCCAACATCCCATATTGATTAATCAATTGGTGAATCAATAATAAAAAGGCAATATTTTTGGATTTTAATATTTCATTTATATTCGTATTTTTATTTAATTGGTTTACTTTTAAACCGTATGCTAACATTGAAAAACTATCATTAAAAAATTTTTTATTTGCAGTTGGCATTCAATTAATGGTAATATTATTACTCAACTTATTTTTAGAAACATTACGAGTATAGCTTATATTAAAATCATTATATTTATGCAAAATTTCTTGTTGTTGATTTATGTCAAGATCGTCAAAATTCAATGTAGTTACAATATTTTGCAAGAATGCATGATACATTTGCATACCAAAAGTATTTTTTGATATTGGCAATGTATTAAATTGAAAATCAATTTTTGAAATTGATTTTTTATTTTTATCGTAAAAGAAATTCTTTAATAATTTTATTTCATCTACATTTACTTCATTAATAAAATAATAAGACGCATAATATCTATCTTTAAAAGTTTTATCATATTCATAAAATCAGTCAAGGTTTGTTAAAAAATCTTTATATTTTTCATTAATGATTTCATAAGGCATTGCATCAATGTAGTTTGAATGTATAAACAAATTGTTAAATAAATCAACCCAATCATGTTCAATATTATCAGCACTACAAATACTTAAATTTTTGTCGTTAAAGTCTAGTAATTTATAAACATCAACGTTGTAACTTTTTAAATATTCAAATAAATCAAAATTATTCAATTGATTTTGCTTTAAAAATTCTTGTTCTTTATCTTTGTTAAGTGTTATATTATTTTTTAATGCTCATGATCTTCTAAAATTTTTATTTAAAAGTAGCATTCTTAAAAGACCTAATTTGAAATCTTTTACATTTACTCAATGAACTTTATTGTTTATATTTCTAATTCCTTGCCAATTAATTCAATAATTTTTTCTTTGATTTATACTGATTGAAATATTTTTTGCTTTTCTTAGAGATTCAATGAATTGTTTGCTATTTATAGACATTGAATTTTTTGAAGGAACGAACAAATATGGATTATTAAAACCATTGCCCTTGTCTTTGCTGGGGTAATATTTGTTGGGATCTTGCTTATTCAATTCAGTGTTTTGATAGTCAACATTTAGTTTATCATCGCTATTGAAAAGTTTATCATTTATTTGAATACTATTACCATATTCAAATTTATAACTTAATTCAGTCGGGCGCAAAACTATATTATCCCTAAAGTCTATTTTAGGTTGATTTTTTGAATAAATTCTAAGAACTTTGGCTCCTGTCATTAAATTTATTTCTTTTAAATTTTGGTTTTTAAAATTTGGTATTGAACTGCCATATTCTAAATAAGGATTTGCAATATTAAGCTTTTGAATATATTTTTTCTTGTCTTCATTAAAATTATTAAATTTACAAGATATTGATATCGCAGATGTCATTGTAAATAATGCTAACATTGAACTTAAAATAAGAATGTTTTTTTTCATTTTTACCACCTTAAAATGCCGTTGCTGTATGAGTGTGAAAACATAAAATAACTATAGTTAATACTAGTCCTAAACTAAATAAAGCAAATGAAGTATATAAAACTTTCGGAGATTGATTTCTAACATGGATAAATTCTGGAACCAATTCAAATATGGACATAAATGTAATAATTCCTCCGCCTAATGCCAAAAACGAAGGGATTATTCAAAACACTTTACTATGTTCAATATATTGGCCAAGCAATCCGCCAATTAACATAAATGGGATAATTAATAAAAATGTTAAGAAGTTGTATAGTATGGCTTTTGATTTCTTTTCTCCATATTGAATTTGACGATAATAAACAACTAAAATTTCAAGTAATATATGAATATTAAATGTTATTATTAAAGGAATATTTGCTTTATATGTTCCACCAATTGATGTTAATGTATAAACATTATATCCCAAAAATAGTCCATCAATAATTCTATGACTCATTAGCATTAAAATAGCGCCTCATGCTGCTCTTGGATTGTCGAAATCTTTTATTGAAAATAAGTGGTCCGAGTGATTGTGCTTATTATGATCTGCATGAACGTCTGTTTTTAAATACTTTACAAAAATGAACCTTCCTAAAATAACTAAAATTAATCCTAATAATGCTGATAAACCAATTATTGCAGCTTGGATTCAAGGAATATTAGTTTCAGAATAAACTCTTAATCCATGCAAATAGCTATTAGATCAATCAAAACTTTCTTTAAAGAAACCTACAGTGCCAATCATTAGAAACATTCCCGTAGCAAATGCATAAATATAAATATTTTTCTTATCAGTAATTTTTAATTTAAATATAGAAATTACGCCCAAAACTAATGAAGGTACGCCCAATAATATTAAAATATAGATTATTAGATTTACAAATGTTGCTAAAGTTATATTTCCGTTAAATGCACTTGTTCCTATAAACATGAAATTCCTTTCTATTTCGTTTATTATACAATAATTTTTAAACAACTATACAATAAAAAATGATAAAATTGTTATATATTATGAAAAAAAAGTTTTATTTTAATTCTATTTTAATTGCTAGCGCACTTAGCTTTATGCCTTTAATAATTTGTAGTTGTCAAACTAAAAAAGATAACTATATTATAGAGGGTAGCAATGCTTATATAGATATTTCAAAAATTTCTAGAATTTTTTTGCATAGGTTAAGCATAGGCCAAATTGCTGCACTACACAACATGTATAAGATTTTTTATTACTATGATAATTCTAATAAAAAGCAATATTATGACTCTGCATATGTAAATGGCCAAAAGCTATATTTAAGAAATGCAAATAAAAATGTTGAATATAAATTAAATTTTCCAATTAAACCTTCTTGGAAACAAGAAATATCAAAATTTGACAATATTAATATTGTAAAAAGCAATAAACCAAGTGATATTAATAATTTTTTAAACCAATATAGTTTTGATGAAGTAGATTCTGCAGGCGATGTTAATGACGAATGATATAGCATATTAGGCGATAAATGAAAAAAAGACTTTAACAGAAATAATGATCCTTATTTTGAAGATATGCAAACTGTTATTTTTAGATTAATTCAAGATACAGAATTAAATTATTCAATAATGAAATCTAAATATTTAATAAATTCTAAAAGAGAAAATGTAATTACACAAAATTTATTTAAAAACAAATACATTCAAGCTAGTTCATGATTAAATGATGAGGAACATGAGCAACATCGTCAATGATTCAAAAGATTTTTAGTAATATATTTGAATAAATTTAATGTCAATGTCGGGGATGTAGATATAGATTGGAAAAATGCAAAAATTGTAAGGTCTTTTTCTGGAACCACTGATTATATAAAATTTAAATTCAAAGATATTAAAGATTGAAACGGAAAATCATTATTAAGAAATGATAAAAAAAATATTGAATATTACATAAATGGTTTTAGAACATATGCAACTAATCAAAAATTCGGTGTCGGCAATCAAGGTTTAAAAGAAGAACTACCATTGTTCAACGATTACGTGCCAAATCCGCTTCTTGAAATTGATGGTATGAAATTTATGAACATTATTGATAATATTAACTATTTTGTTAAAGGCGCAACTGATATTAATTATTGAAATGCAAAAGGATTAATGTATCTTTTTCAAAGCTTTAAAAATGAACCTGGTTTCTTTAAATTAGCAGTTCCCGAATATAAAAAAAATGAAGATAAGGAATATAAAATTATTGATTTTGAATATACTCCATATTTTGAAACAAATCAAATTTTTAAAGCAATAGTAAGGGTATTTAAATTAAATGGTACATATAAAGATTATGTTTTAATATCTTCTAATTTTGATGATCACGGTCACAGATTAAAAGGCTTAATTACTAAAAATGCTTTACCAAATGAACTTAAAGCTTCTGATATATATTCAATTAGAGCAGATAGTGAGCCAATTAAACAAGGAATCAAATTGGATGATTTCATTGACAAGAATAATAAAAATAGTGTTTTTAGATATATGTTAAATGAATCTGCAAAAAAACTAGAAAATATTTTTGAATATTGAAACAATAATAATAAGCAAAATTATGAAGTTGCTAATTTATTGCTTGCCAAAGATCCATTCCAATTAAAATTGTTAGCTTCTTATTTAAATAACTACTTATTAGCATATGCACTTGAAAATAAGGCAGGTGAAGTATTTAGTGGAATTAAACGCATCGATCTTAACATAGTTAAATTGCCAAATCAACCAATTGGAAGATTATATTTAAAAATGGATTTTGTTTCATATGCCAATAACCAAGATTTCAAATTCAAATCAAAAAATGAAAAAATTGTAAAAAGTGTTTATTTATATTGAAATGGATTTAAAGGATATGACCAGCAAAAATATGGAAATGAATATTTTACCATTGATAAAATTACAGATGGAGATAATTAAACATGAAAAAATCAATAAAATTGCTATCCTTACTTTGTACTTCTACAATAATTTCATTGCCTGCTATTTCTTGCACTATCAAAGTAAATCACGATAACGAAGACAAGGAAAATTTAGATTTTTCAAGTGATTTTAGTGGCAAAAAGCAAAAAAATGAATTCAATTTCAAAAATAAGATTTCTCAAAATATTTATGATTTGTATCAAAATAAAATTGCTATTTTATTTAACGATGTAAAAACTAATTACCGGGGATATAAAAAAATATTTGGATCTTTAACAAGAAACTTAAACACTCTAAGGGCTAAGATTAATAGCTTAAGATACGAACAAAGTTTAAAAGAAAACAAAAAAATATTAAATGATTTTTACAATAATTGATTAGAAATAAAATCATCAATGCTAAAAAATAATCCATTTGCATTATATTTATTCAAATATAGTTTAATTTTTTCTGATGTGGATGCTGTATTAGCTGATACAAATCTTGTTTTTGAAACTTCTGAATTTTTAAAATATTTAAAAACAATTGATGACAGACTTGAAGGTAAAGACATTGATTTAGGTCAAACTCAACATGCATTACAAGCGGTTTGAGATTTTATTAAGGCGCATATATTCAATCCAAATAAATTAACCGAGAGAGAAATGCTTGATAAAATTAACATTGAAGATGATAAAAACTCGCATAATCACTCACATGCAATCATAAATTTAACATATGAAATGGGTTTATGGCATGAAATGCTGGCAAAAAATAAACTTCAAAATTACGAAGAATTTCAAGAAGAATATAAAATTGCTAAAAAACACATAATCGATAATATAAACAATATTGATGTAAATAATAATTATTTAGCATTAAAAAAAGTCCTAGATAAAGATTGAAGTTGGGGAAATTCTTACAACTTATTAAATGATTCATTTTTTAGTAGAGCAAAAGAAATTTTAAAAATACTAAAAGAAAATTTGATAAGTATTGCTAAGATTGTGGGTTTAACTGATAAAATCAATTTAGACTAAATCTTTAAATCATAATTCCTTTTTAAACTAAAAATAAACGTAACTTGTTATAAACCTGGACAAAAAATTAGACTCGTAATGAAAAAAATGCGAGTCTTTATTTTTATTATTGAAAGGTTAAATTATTATGGAAAGACAATTAAAAGAAGATGAATGATATCAATATTTTTTATAAGCGTAGAAAAATGTGGGGTTAATTCCAAATAAGGAAAAAAAGCAAGATCATAAAGGTCTTGCTTTTTTTCCTAGAATGAAAATTCAAGTGCAACACAAATTTGAAATAAAATTTAGTTGTGAGGCATTTGAATTTTCATTTTGGCAATAAAAAGCCGTTTGGCTTTTTATTTTTTAAATTCTTTTTTAACGTACTTAGAGTTAAATAAATAAAGCAAACTTCCTCAATCTAAATCAAATTTAAGTATGTCCCCACATTTATAATTAATTTTAGAATCGTTAGCATTAATTATTAAATGGTCTGAAGATCCACCTAATATTTTTAAATCTTTATCATAAGGTATCATCGCATCAAACATCGTGTCTAATTTGCCGATTGCACAAATTATTCTTTTAATATTTCCAATGTCTTCAAAATAAGGCTTATTTCCAAACGCATCAATTCCACATTCACCAATTGGCAATGAACTTTTGTAATCAATTTCTACAATTTCAGCTTCGCAACTAAATGCGTCTCGATACATACCTTTTATAGTTTTCCTATACATATCTTCTGTACCAAAAATCATTGCAAATCCCATTCTTAAGAAATTAATTTCTTTAGGCATACGATTTTCTCAAACTAGGTGCAAGCTTAGTGAATTTCCGCCAGACATATGAGTTAATTTAATTTTTAGTTCATCTTCAATTTTGTGTTGAATATTTACAAATAATTTCATTGATTCATCTGAGGGAACTCTAGCGCCATAGCAACCGAAGTTACAGCCTAACCCAATTAATTTAACACCTTTTAGTTCATGTATTTTTTTAGCAGTGCTCAAAGCATCTTCAGGTAAAACACCTTCTCTTCTATCGCCTAAATCTATCATTAAAACTATTTCATGTGGTTTTAAATTATTTTTAAGACAATATTCACTAATTTTTTCTATTACTTGAATATCCCCATTTAAACTTGAATCACAAATTTTTACAACTTCAGGAATTTCTGAAATCATTGGTATTCTTAATAATTGTTTTTTAACATCTTTAAATTTATTAAAGATAGTAAAGTTTTCAAGTCTAGAATCACCAAAATACCTAATTCCTGCTTCATAATATAGTTCAGCCATTCTTTGATTTCCACAAAAACCTTTAGTAACGGCAAGAACTTCTATATTGTGTTTTTTACACATTTCAATAGCAACTTTTATATTATTTTTAAATTTTTTCTCATCAATAATTATTTTGGGATATGACATTTATTTACCTCTCAAATATTGTTTATGTAATGATATTTTACAATAAATAGCTTTAATTATTAATTAGTTAGCTCTATAATTTTAAAATAAAAAGGATGTTTATAAACCTGGACAAAAAAATTAGACTCGTAATGAAAAAAATACGGGTCTTTATTTTTATTATTGAAGGGTTAAATTATTATGGAAAGACAATTAAAAGAAGATGAATGATATCAATATTTTTTTGATATAAACAATATTTAAATCATGATATTTCTAAAGATTTTTTATCATATAAATATTGTGAAATTAGTAATGGAAGGCAACTAAATAAATATAGTTTAAAACTAATTAAAGCCAAATATAAACTCTATAATTTAGGTATGAATATAAAATCACAAACAGGCAATGCCCCAAAAAAGGTAAGGGTTCAGGTCGTCCAAGAAAAAGAGATATTGAACAAGAAAACAAACTTCGCAAAAAAACTTTAAAAGAAATGTCAAGAGAACAATTTGAATATCTCATTACTGATATTTTTTATGAAGAAATTTTAAAACGCAACAATAGCAAAAATTTAGAAGAAGTTGTTGAAAAAATTAAAAAGAATCATAATTTTTCAATATCAATAAAGCAAATTATCACTATTTTAGGGATTTAAAAATCTACTTTTTATTATAGAATTAAAAAATTTAAATTGCCAGCCAAAAACAAAGCAATTCAATATGAAAAACAAATAAAAGAGGCTTTTATTGAATGCAAAGGAAGATTTAGAAGGGAAAGACTTTCACTTTTTTAAAGAAAAAGTACCAAATTGATATAAACCCAAGGACTCTGGGCAGGATTATGAATAAATTGGGTTTGTTATGTAAAATAAGAAGAGATAAAAGAAAAAGAGAAATAAAGAATGTTAATGCAACATTCAAAAACTTAGCCAATAGAGATTATGATGGCCAAATGAATGATATATATGCTACTGATGTGACATATATTCCTTCACCCACTGATGTTATAGAAAATCATGTATATTTATCGGTTTTATTGCATCATAAAACAAAGAAAATTGTGAGCTGGAATTTATCAAAATATAATGATACTAATCTTGTTTTAAAACATTTTAAACTAACTAAATTTCCTAAAAAATTCATTGTACATTCAGACTACGGATATCAATATTCCTCTAAGGAATTCCTTGATTACATAGATTCAATTAATGGCACTATTTCTATGTCAAGAGTTGGAAATTCTTTGGATAATAGAGAAGCAGAATATTTCTTTTCTATTCTAAAAACTGAAATATTTTCAAATTTTTTCAAAAAAGCAAAAAGTTTAACATTTAATGAATTGCAAACAGAAATAGACAAATTTATTAATTGATATAATAATGAAAGATTTATCAAAAAATTTGATTTAAAAACACCCCAACAAATGCGGGATGTTTATATGAACAATAAATCTATTTGAGTCTAAATTTTTTGTCCTAGTTTATTTATAGGCTTTTTTTATAAGTTTATATTATTTTTTATCTAATGTTCTAAATCTTAATGATAAGCAGCTAAGTCCACCATCAAGTTTTCTATATTCACTTGTATCACAAGTAATAACTCTATAAATACCTAAATCTTGAATAGCTTTTAAAGTTTTTGGATATCCTTCAGGTACTATAACAGTTTCATTAACTCAAATACAGTTTGCGGCATATGCTTCATCGGCAGGAACTACAATTTTGTTAAATGAATCAAATGTAGGATAGTTAACAAATTCACCTGCAATTAATAAGTTATTATGTTCAAGATAGTTAACTCCTGTTTTAAGGTGCAACATTTCTGTCATTGGTACTGGAACTAACTTCTTACCAATTTTTGCTAATATATTGTGGAATTGTCTAATTCCGTCACGATTAGTTCTTTCGGACATACCAATGTAGTATGTATCTCCAACCATCATAACATCTCCCCCGTCAACAGTTCCGGGAGCTTCAATTTTTGCAATGTGTCCATCATCAAAGTATTTCTTTAATGCAGGAAGCATTTCAACTTTTTCTCCATTACGTGTTTTAGCACCTGGATTAGTTAAAATAGCTAGTTCACCTGGAATAATAACAGCAGTATCTTCTACGAAGCATGAGTCTGGATATTTTTCATTTAAAGGTAAAACGGTTATATCAACACCACAAGTCTTTAATTTTTCAATATAATCTTTGTGTTGTTTTTTAGCAAGTTCATAATCTGGATGTAAGCCAGCTGATGAAATACCATTTACCATAGCAGCACATGGTGTTTTAACAATAATACTATTAAATTTATTTTTTAAAGGTTTTTCACATGATTTGCAGCAATCTTTGCAATCATTACATTTTTTACAATTACACATGTTTTTTCTCCTTAAATATAAAATTGTCGTATATATAACGCATAATTTTACTATTGCTATACATTTATAATTATGCCATTTTATGGTAAAGTAGAAATTTAAAATATTGAATTTACATAAAATTGTTTGTTCCTAAACAAATTGATAATCTTTTACTAAAAAAATTATAAGTTAATTTAGTAAGAGATTTTTTATATTTTTTGCCAAATGAAATCGTTTATTTTTTTGGTTAATTTCTATAAATTCATTATAAATTTTTGTTTGATTTTTAATAATTTCAATTGTTGTTTTATTTTGTTGATCTAAATACTTTTGATTTAGCTCAAAAAGTTTTTGATATCCCTTGTTGGTTTTTTCTTTTTCAGCTCATAAGGCAACTAATGCACCACTTTTGGCATCAATTGCATAATAAATATGAATTTTTCATTGGTTCCGAAAAATCTATCTTGGCAAGCATCTACTTCAATAATTTCACCAAATTTATAATTTTGGTTTTTATTTGAATATATTGACAATTTTTTAGTGTTTTCAATCAATAAAATATTCGCCTTATAAATTAATTTTTCACTATTGCTAATTTTGCTGTCATTAATTTTCAAATTTGAAAAATATTTTTTGCAATTCTTTTAATTTTATGGGTAGCATAAGGACTGCAAAAACCATGCTTTAATCAAGCATTATATAAATACGATAACGAAATTTTTGACTTTATTTCTTCACTAAGGTCTTCATAAAAATCTTTAACAGTAATAAATTTTTTAGTAACATTTGAATTTATTTTTTGATTTTCAATTTGATATTTCAAAAATAAGTCTTTTGCAAAACCGTTTTCTATTATTTTTGCATTTTGATTATTAAAGTTTTTATGTCTAATATTTACTTTAATTTTGCTACCTTGCATATTATCTTTAATAATTTTTTTATATCTACAAATTGTAGGAATTGATAACATTGTTTTTTCTTGCAAATTTTTGGAAATAAAATCTAAATTTTTAATCTTTTTAATTTCATATTTTGAAATAATTGAACCATTAATTTTTGCCATAAATAATACTCTCTTATTTAATTTACAATTTTAAGTAAAAGATTATCAATTTGTTTAAGAATAGACATTTCTTTGTGAAAGCGAACAAATCAGTTGCGAAAACGAATTTTTTATGAAGAAATTTTAAAACGCAACAATAGCAAAAATTTAGAAGAAGTTGTTGAAAAAATTAAAAAGAAGCATAATTTTTCAATATCAATAAAGCAAATTATCACTATTTTAGGGATTTAAAAATCTACTTTTTATTATAGAATTAAAAAATTTAAATTACCAGCCAAAAGCAAGGCGATTCAATATGAAAAACAAATAAAAGAGGCTTTTATTGAATGCAAAGGAAGATTTGGAAGGGAAAGACTTTCACTTTTTTAAAGAAAAAGTACCAAATCGATATAAATCCAAGGACTCTAGGCAGAATTATGAATAAATTGGGTTTGCTATGTAAAATAAGAAGAAGCAAAAGAAAAAGAGAAATAAAGAATGTTAATACAACATTCAAAAACTTAGCCAATAGAGATTATGATGGCCAAATGAATGATATATATGCTACTGATGTGACATATATTCCTTCACCCACTGATGTTATAGAAAATCATGTATATTTATCGGTTTTATTGCATCATAAAACAAAGAAAATTGTGAGCTGGAATTTATCAAAATATAATGATACTAATCTTGTTTTAAAACATTTTAAACTAACTAAATTTCTTAAAAAATTCATTGTACATTCAGACCACGGATATCAATATTCCTCTAAGGAATTCCTTGATTACATAGATTCAATTAATGGCACTATTTCTATGTCAAGAGTTGGAAATTCTTTGGATAATAGAGAAGTAGAATATTTCTTTTCTATTCTAAAAACTGAAATATTTTCAAATTTCTTTCAAAAAGCAAAAAGTTTAACATTTAATGAATTACAAACAGAAATAGACAAATTTATTAATTGATATAATAAAAGATTTATCAAAAAAATTGATTTAAAAACACCCCAACAAATGTGGGATGTTTATATGAATAATAAATCTATTTGAGTCTAAATTTTTTATCCTAGTTTATTTTACTTACTTTTTTAAATTTTGTTCATTCCTTATATTTTTTACACGGCCTAAAAATAAAAAATATTTCTTAGTATTATATTGCTAAGAAATATTTGCCAAAATGAAAATTCAAGTGCAACACAAATTTGAAATAAAATTTAGTTGTGGGGCACTTGAATTTTTTTAAAATAAAAAGCTCCACAAACGTACCAAGGGAGCCTTTATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGTTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCGAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAGTTTTCCAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGAATAAACACAAATAAATGAAAAATAAGAAAATCCGATAAATTAAGGCAATATTATAAAAAAGGAGGCAAAAGAACTGCTTCGGTTCTTAGTCGTCTAATAAAAAGTGCAAATTATGTGTTTCCAATATGAACAAGACCAAAAAATATTGATTTAAGGTTGGAATACGGGCATTGAGAGGCAGATTTGGTTCTTGGAAAAAGAGCAAATGGCTATAAAAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGCGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGAAATGGCATGATAAGAAGAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGAAAATCTGCATATGAATTGTTTATGTATGAAAATTTTGGCGAAATAATAAACTAGAAAAATTTAAAAAATATAACATGTTGTTTATCACAACGTTTTTTCTATGCAAAAATTTTAAAAATCGCATAGAAATTTTAATAACTTAATATAATAAAAACATCAAGCACAAAAGTGTTGCACTTGATGTTTCATTCTGGGAAATATTTCTTAGTATTATATTGCTAAGAAATATTTTTTTAAATTATTAATCTATTTAATTTTTGCTCTTTTGATCACTTAATGGATCAAAAGCAACGTAAGGATATTTATCTTTTAATGTAGATCATATGTATGAAGTATCAATGTTTTTGTTGGCCTTTTGAATAGCTTCACATTGTTGATATGAATTTATTGCATCATATATTGCTTTATATGTTGTCGGGTTGTTTTTTTCTCATGCAAAATATTCTTTGATAACAACTTTATCAATTTTGTTTTCAGTATAATTTGAAAAAAAGCAATGAACTATTTCATGTAGCAATGAATAAAATATATATGCTTCAGAATTAAACATATCAGAAAGGAAAATATACCTTTTTGCTCCCTTTTTCAAAGCAATAGCCCTTATCAAACTATTTTCAATAAAAGGCATCGTTACTAGATTAATTCCTTTGCTTAATAAAACTCTTTTAATTGTTTCCATTTTTTCTTCAAATGAACAATGTGTATTAGACATGATCGTTAAAACTTTATGATAAAGTGAATTAAATGAAGATTTTCTAAAAATTCCCACGTTTTCATCAATATTTTTATCAATTGAAAGTAATTCGCAAAATCTTATTCAAACTTTTGTATTTGGGTTTGAATATGTTCATTCTTCTGCTGAAACATTATCTTTTAAATATTTATCATAGTTAGCCAAGTCAGCAACACCAAAAAATCTTTTTAGCATTATTAATTTAACATGTAATGGACTATTTTCATGAATGCTAATGTTAGCTGTTAATGCTAGCTCTGGATGATTTATTAAAAAATCAATACCATGTTTCTTTAAATCATCTTCAATATTCTTATTTTTCTTTAGTGTTTTTAAGTTATTATAAATATGATAAACTTCCGTTAATGTGCCAATTTCTAAGTGAAATGCATATTCAAGCGCTTCAATAATAGTTGCGCTAACGTTATTAACTTCATTATTCATAATTGAATTGATATGTTTTATTGATAATCCAAGTCTTATAGATAATTCTTTTTGAGTCATTGAAAACATTTTTAAATAGTATTTTATTTCATCTGTAAAATCATTGCCATGAATTTTAACGTGCTTCATATCACAGATGTGCTCTTCAATCATAAACCCGCCTTAACTTAAACTATTTTTTTATTTTTTATAACTATATTATAATTTTTTTTAGTTAAAAAAGTTTATTTTTGATACAAAAAAATATACAAAAAAGTATTTTTATATTGTTTTTTGTATATTTTTTTAAAATGATAATTATTGTTAATATATTAAATTTAATATTCCTAATCCACTATATCTTTTGGTGCAACTGCAAAAGGTAGAATATCTTTCATTTTATAATGCTTTTGTTGCTTTAAATCAAAACTGTATAAATATACATCGCCATCTGGTTTTAAAAATTGGCTAATAACTTGGGCACAAGCACCGCAAGGATATAGCAATTTAGATAAATTGCTAATTATATGTATTTCTTTAAAATTACCAACTCTAGCGCCATATGCTACAGAACCAAATAATGCACTTCTTTCAGCACATAATCCAGATGGAAATGCAGCGTTTTCAGTATTGACTCCCCAATATTCTTTACCATTTTCTTCAATTGCACAACATGCAACTTGAACATGTGAATAAGGACTAAATGAATATTTTAATTTATTTTTTAATTCTTCTATTGTCATACATATAATTTTACAATATATTTGCAATATAAAATTTATATTAAAATAAACATTTTTTAAAAAGTGTTAAGTATTTTTGCTTAACAGTAAAAAATGTGCTAAAATATATACTAACTTTAAAAATAAAATTATTAATTAGAAAGGAAAAACCATGGTTAAATTAAGATTAAAAAGAACTGGTAAAAAATTCTATGCAACATATAAAATTGTTGCCGCAGATGCAAGAAGCCCACGTGATGGTAAATTTATTGAAGAATTAGGCTACTATGATCCTAATTCAAAAAAATTAGTGTTAAATAAGGAATTAATTATTAAACACTTGAACAACGGTGCAAAACCTTCAGACACTGTACGTAATTTGTTAAAGCAAGACAACTTCTATGCAGACTATGTTGCTTCTAAAAAATAATTTTAATAAATTATGAAGATAAACATTCTGACACTATTTCCAGATTATTTAAATGCTTTTTGCAATCAAAGTATGATAGCAAAAGCATTAGAGCTTGGACACATTGAAATAAACATCATTAATTTTAGAGATTATTCATTAGATAAACATCATAAAGTTGATGATAGCCCTTATGGTGGAGGCGCAGGTATGTTGTTGCAAATTGAACCAATAGATTTAGCACTACAGCCTTTAAAAAATAGTCACAAAATTTTACTTTCACCGCAAGGAAAGGTATTTAGTCAAAAAAAAGCACATGAACTTGCCAAATTAAATGAAATTACTTTAATATGTGGGCATTATGAAGGCTTTGATGAAAGAATAACAAAATTAGTGGATGAAGAAATATCAATTGGAGATTTTATCTTAACAGGTGGAGAAATTGCAGCAATGGCAATTTCAGAAGCAGTAGCAAGATTAATACCAGGAGTATTAAGAAAAAATAGCTATGAAAATGAAAGCTTCGAAGGAAATGGATTACTAGATTATCCACAATACACAAGACCTGCCGTATATAAGGGAATGAAAGTACCAAAAATCTTATTAAGTGGAAATCACCTAGAAATAGAAAAATGAAGATTAAAAAAAGCTCGGGAAATAACCAGAAAAAAACGTCCTGAATTATTAAAGAAAGGGAAATAACATGAGAACAAAATTGTTAGAATTAGTAGAAAAAGATCAAATAAGAGAAGACTTGCCACAATTAAGAGAAGGTTATAACGTAAGAGTTCACGTAAGAATTAAAGAAGGCGATAAAGAAAGAATTCAAGTTTTTGAAGGATTAATTATTGCTGAATATGGTACAGGAATCAATAAATCAATTACCGTAAGAAAAGAATCATATGGTGTTGGAGTTGAAAGAATCTTTAAAATTAACTCACCTTTAATTGCTCAAATCGAAGTTATTAGACGCAATAAAGTAAGACGTGCTAAATTGTATTACATGAGAGATTTAAAAGGTAAGAGTGCTAGATTAAAAGAAATTAAAAACTCAAAATAATAAAAAAATAGGGCGTAAGGCCTTATTTTTTTATTATTTAAGGCAATAAATTATATTACTTTAATTAACAAATTATTAACATTTTATTAACAAAAAAATTCAATATAATTATTGGTAAAAAATTTTGCCATTTAATTAAATTAAATACAATTTTTAATATCTTTTTTTCGACTTAAACTATAATCTTGTTCGAAAGGAAATATTAAAAAATGAAAAAACATACAAAAATTTTATTAGCATTAGGAAGCATTGCAACTTCAGTTGCTGCAATACCATTAGTTGCTGCAGGTTGCGTAAAAACAAAAAAACCAGAAGTTAAACCAGAAGAACCTAAAAAACCAGAAGGTGAAAAACCAGGAACAACACCAGAAGTTAAACCAGAAGATAATAGATCAGAAGAACAAAAAGAAATAGATAATTGAATAGAAACTATTAAAGACCAATTTGAATTTTCTCCTTACAACAAAGAAGAAGTATTAAAATCATTAAAAGATAAAACGGGAACATTAGTATATAGTTATAAATCTCATAGTATAGTTTTAAAAGATAATAATGATGGTAAAGTAAATTGATCTAACATTGTCTTTTCTCCTAAAGATGAATCTTTATTCTCACAATATCAACTAGCCAATCCAACAAAGCCTACTTTTTATAATAAAAAATACAAGAATAATTCAATAAGTGGTTACATTGACTATGAATTAAAAGACAATAAGATAACATTAAAATTTAAAGGAGCTAAATTTGTTGCGAGAAAAGACCCTATTATTGGAACTAAAATTGTAAGCAATGAAATAGATCTATCAGAAACACAATTAGATAAAATTATAAAAGATCTTAAATTTGATATCAAAGACAAAAACAAAGAAATTAGCAAATTAACTCCTGCCGATGTTAAACTAGAAGGCAATCTAAACGGTGCAAATATTACTTATAATTTTGCAGCAAAAAAAGGTGATGAAACAACACTTGTTTTAACTGTTAAAATTTCAAAGAATTCCGTAACTCAAACTTTTGAAAGAGAAATTAAAGGATTTAAAAAACAAGAAGCAAAACCTGAAACTAAAAAAGAAGACGAAGAATTAGATCCAAAAGTAAAATTGATCAACAAAAAAATTGAACAAAACAAAGACTTATTTACGTTCAAGGTTTGAGACCAAGCTAAAAAGCAAGAAGTAATTGAAAAATTAACAAAAAAGGAAGCTAAATTATTCTATCTAGGAAATCATAATATTATTATTGAATTCGATAAACAAGAACACGATAAAGCCTTAACTCAAGTTGTATTTGAAGGAAAATATGGAGCGTTTTATTCATTTGGTCGAGTTCAATTAGTAAATCACCTACACCACTACAAAGATGAAAAAGTTGCAAAAGATAAATTAGAATTATCTAACTGCTTAGAATATGAAGTAAAAGATAATAAAATTACTATAACTTGAGCTGGTGGACAAAAAGATAATGGAGTAGAACCTTTTATCTTTGGAACTAAAACTGGTTCACAAACATTTGAATTAAAATAACAAAACATAAAAAAGTGGGCAACATTGCCTACTTTTTTATATATTTTTTAACAATAGGAATTTATTAAATTCTTTTAAGTATGTAAAAATCTTTTTTGCCTTTTTTTAATAGCGCATATTGACCATTAAATTTATTAAATGTAATTGAATAATTTTCATCGTTTATTATATTGTTATCAATTAAAAAAGTATTTTTTGAAATAAATTCTCTTGCTTCTCTATTTGAAGAAACAATTTGATTATTTTTTAAAATTTCAATAAATTTTTCGCCTTCTTTAACTTCAACACATGGCAAATAGCCTAAAAGCATCGAAATATCTTCTATGTTTAATTCTTCGTTACTTTTATTGAATAAAATCTCACTTATTCTAATACATTTGTTTGCAATATCAACTCCATGAATGTCTTTAACCAATTCAAAAGCTAATTTTTTTTGGGCAATTCTTTTGGTTTTATTTTCATTGTGTTGAGTCATAATTTCATTTATTTCTTCAACTTCTAAAAATGTTAATCATTTTAAATATTCTTCAATTTTTACATCACTTTGATTTAATAAAAATTGATATATTGAATATGGGCTAGTCATATTTGGATCAAGCCATAATGAACCTCCGCCAGTGCTCTTACCAAATTTTTTGCCATTTTCATCAACTAATAAATTAAATGTCATGGCAACAGCATCAGCAGATTCTCCATGTAATTTTCTAATAATTTCTAGTCCAGTTGTAATATTTCCTCATTGGTCTGAACCACCCATTTGAATTTTTACATTATGATTTTCATATAAACATTTAAAATCTCAACCTTGAAGCAAGGTATATGAAAATTCTGTAAACGTTAAACCATTTGCAAGTCTAGTAGTAATAGAGTCTTTTTCAAGCAAATAATTTACATTAATTAATTTACCAATATTTCTTAAAAAATCTACTACTGTTCAATGTTTATAAAAATCTAAATTATCAATTACTTCTAAACCAAATTTTTGTAATTGATTTTTAATTGCATTTTTGTTTTGTAGTAGTGTTTCTTGATCTAGAAATTTTCTTTCAGAATTTCTAAAACTAGGATCACCAATCATTCCGGTTATTCCACCCAATATTGCAATTGGCTTGTATCCTGCTTTTTTAAAACGTAACAAACTTAATATTTGTAGATAATTTCCAAGGTGTAAACTTTTAGCAGTTGGATCAAAGCCTGTATAAACACCTGTCCCTAGTGATATATTATTAAATTTTTCTTCACTAGATATATTATTTAAAACACCCCTTGATTTCAAATCATCTAATATATTTTTTTGCATATCTGTCCTTTTTGTTATAATGTATTAAATTATTTATTAAAAATATAAGTAATATAGTAATTATATATTAATTTTGTTTATTTTATGGCTATGAATCTTAAAATAAACAAATAGAAGGACATTATGAAAATTAAAATTATTATAGACTCATCAGCAGGAATGTCACAAGAACAAGCAAACAGCTTTGGATGAGATTTAATTCCAATTCAAATTGAAGTAGGAAATGATATATATAAAAGTGGTATAGATTTTGATACAGACAAATTTGAAGAACTTTGAAGAAATGACAAAAAAATAGATGCTAAAACTTTTGCAACTCCCCCCGGAATAGCCATGCAAGTTATAGAAAAATATTTTAATGACTACGATAAAATTTTAGTCTATCCAATTTCAAATGCGCTTTCTTCGCAATGTGCTTCACTAACAGCACAATTTGCTTCAAATCCAAAAGTTCATGTAGTACAATCAAATAAAATTTCTTATCTGATTGTTAGAGACTTATTAATGTTTGAAGAATATATTAAAGAAGGAAAAAGCTTCGATGAGGCAATAGAACACTTTAAAAATAATAATGAAAGATTACTTCTAATTCCGGAGTTTAACGATGCCTTACTAAAAGGCGGACGTTTATCAAAAGCAGCAGCCGCAATAGCAAAATTATTAAAAATAGTTCCTATTATTAAATTTGATAACGGAATTTTAGAAAAAGATGGTATTGGTAGAATCTTTAAAAAAACTCTAGAAAAAAATTTAAAAGAATTATATGAAAATACAAACAAAGATAATGAAAACAATTATCTATTAGTTGTAAATGCAAATAACGATAAAATAGATGAATTAATTCAGTCGTTTAAACAAATAACCAATAATTTTCCAAGGATTTATACAATTCAATTGAGCTGCGACATTGTAGTTCATGCTGGTATTGGCGCAATCTGTTTAACATTTGCCGAAGTTAGTCCTAAAATAAAAGATAAATTCTTTTTATACTTAAAAAAACAATAAATTAAAAATACCAGGTTATTACTTGGTATTTTTAATTAGTTTATTTCTTGCTTTCAATTGGGTAAACTGATGCAAATTTTCTGTTAATTCTGCTTTCGAATTTTACAATACCATCGATTAAAGCAAATAAGGTGTCATCTTTTCCTCTACCAACATTTTGGCCTGGGAAAATTTTTGTTCCTCTTTGTCTATAAATAATGCTTCCTGCTAAAATAAATTGGCCGTCGGTTGCTTTTACGCCTAATCTTCTTCCCGCTGAATCACGGCTATTGGCGGTTGTTCCACCTGATTTCGTATGTGCCATAACTATTTTTCCTTAATTTCTGTAATTTTAACTCTGGTAAATGGTTGACGATGTCCTAATTTACGTTTATGAGTAGATTTAGCATTATGTCTATAAACAACTATTTTTTTTGCTTTTCCTTGTTTTTCGATGGTTCCTAAAACAGAAGCTTTTGGTAGATAAGGTGAGCCAACTTGTTCATCAATAACTAAAACTTTATCAAATGAAACTTTGTCACCTTCGTTGCCATCAATTTTTTCGATGTAAATTACATCACCGCTTTTAACAAGTAATTGTTTTCCGCCGGTTTCAATAATAGCAAACATTATGAAGCCTCCTTATATTTTGTCGCATCCTTAAGGTACTATTGATATAGTTTTAAAACCTTTTTAGTATGGTTACTCTATAAGTAACAAATACATTATAGCAAAAAAATATTTTTTTAATCAGTAAAAAGTGTTTCAAAAAATTTCTTTTATTTTTCAAACAATTTACTTACTTTTTTAATTTCAATATCTAGTTTTTCTATTGAAGCAAATATTTCATAAATTGTTGAACTTTTATCCTCTTCGGCGTTTTCTAGTAATAATATAGTAAATGTATCAACTGTAATATTTTTTTAAACTTAATAAATTAATTTATTAAAAAACCCCTATGCTAATTAATAGTAGTATTGCTTGATTAAAAAAAGAAAAACGATATATTCTGGCAAATAAAAATATTATTTCAAACCTTTTAGTTGATTTTAATTGCTTTTTGAAAGCATAGAAAAATGTGGGGTTAATAGAAATAAGAAAAAAAGCAAGATCATAACTGTCTTGCTTTTTTCTATTATTCATAAAAAGAAAGACTCGCATATTTTTATAGAGTATGAAAATCTATTTTATAGATCTTTTTTTTATTTTTTAATTTGTCACTGCCTATTTTAAAGCCTTCTTTTATTCCATTTAATTTTTCTTTAATTTCCATATTTCAAATTTCTTTGTCTTCGTTTTACAATTTTTAATATTCTTCAATAATATTTCTTGCTTCATTAACATAATTTTCTCTTAAATACGTTATTAAATCGTGAAATCTTCGATCTTTTGTGTTTTTATAATTTTCTTGTAAAATTAGTCTTAATTTTCTATTGCATCTATATATGCTTGGATTAAAGATTTCACAATTGCTTTTATTTGCATTTTAAATTCTTTAACTTTGCTTCTTAATTTCCCAAAAACAGATTTATTTATTTTTTTTGTATAATATAAGTGTAAATAATGAAATATAAAAGATAAGGAGATAATGTAATAGAAATAGTTGAATATTTACAAAAAAGACAATTTAGATAAATTTTTAAAAAGAGAAGATTTACCAAACTTTTGACCTATTTATGTTTATTGTGGGTCTGCCGGTTGAATTAAACCTTGAAAGTGAATTAGGGATGGATATCCTTGCTTAATATTTGGACTTACATACCTGATACTTGGGATAACCAAAAAAATAGGACATGCCGTTGTTGCATATGGTATGTATGATAATGGCAATAAACTTCCTTGCCATTATGGGTGAGCAGGCAGTTCTCAAGTTATTGTTTCTTCGAGTCTATCTGGCCAATTGTTTTTATTAGCTATAAAGCCAAATGGCAAAATTAAAAAACAAGACAACATTTTACTAAAAATGGAATTAAATATTCGGGAAAGGATTTTAAATAATGAAGAAAATTACATTAAAATGGTTAACAATTTTAGGAGCAACAACGCTTACTGCCTTGCCTTTTTGTGCAATTTCTTGTAGTAATGAGGAAAAAGAAATTGAAAAAGCAGAGAACATTATTGGCGATAAAGAACTAACATTTAAAATAGATGATTTAAAATATGAAAATTCTGATAATTCTATATTGCAAAGTGCTAGAAGTATTGCAGGACCATGGCGCAAGGACTTTCTTAAGGTTGTTATAACGTCAAAATCAAATATTGAAGAATATTGAAAAGATAATTGAATTGTAGGAAAAATAGATTTAAAAAATAAGGCTATATTATTTTTAATACCTAGAGAAGTATTATTAGATGCTGATTTTATTAAAGAAAAAAATATGGATCTAAAATCTTCTTACCAAAAAGCAAACAAAGCATATTTAATAGAAAAACCAAAAAAAGTATATTCAAATCTAAAAAATAAACAAATATTAATAAATAACTACACTATATTTTTTGATGATATAAATAATGATAAATCTCCAAGAGTTTCAATGGCGATAAAAGAATTAAAATTTAATTTTGATCAAGACCGAAAAACCATAGAAATTAAACTTGGTTTAAGATATGAATTATCACAAAAACATAATAGGAAGTCTAATATATTTTTTCTTTCTCCTAAGGCAACCTTTAAATACATTTTAAGCGAAGAAATCAAATAAATTTATTTAATCAGCAAATATTGATTTAAAACATTAAAAAAGGGAATCATAGAAAAATCTGTAGTTAATTCAAATAAGAAAAAAGCAAGATCATAACTGTCTTGCTTTTTTCTATTATTCATAAAAAATAAGTTTAAAAAAATTAAAAAATCTTTAAAAGGAATTTATGGGTGCTAATAAAGAATTTATTTAATGCAGCAAGAGGCTAAATTAAACAACGATTATTCAAAGCTCTTTAATTCTTATAAAAACCAAATTAAGAATTTAAATAATTTAAACATCGAAAATAAATTTCTAAAACAAAGAATTTTAAAAATTATAAATAATGAAATAAATGATTTTAAAAAAGTTGGTAATAAGATAATTAAAACCTTACTAAATGCCTATAAGCAAAACAATAATGCATTTACTGGCCAAAAGAATTTCGCTTCATTTTTTAATGAGTTTTAAAAACAAAGTTAACTATTTTATTAAAAACAATAACAAATAAAAAACACCCTTATAAATATAGGGTGTTTGTGTCAATAAAATTAAACTCTTATTTAAATTTTAATTATTCAAAATCGTTTTGATTTTCTTCATCATCAAATGTTTCTTCTTCAGCAACAAGTTCTTCGTCGTTTGGAGAATAATCTTCTTCAACTGATTGTTTATCATTGTCATATTCTTGCGCTGTATTAACAACTGAATCAATATCAATAGGTTCAGAAATTTCTGTGCCGTCAATAGGAGTTGTTGTAAAGTAATTAATTGATGGTTTTATATCATATTTTGAATGTTCTTCAAATGGGAATAATGCTGTTCCTGATGGAATCTTTTTACCAAGAATAATATTTTCTTTTAAGCCAGATAAATTGTCAATTCTTGATGAAATAGCAGCGTGAACTAATATCTTGCTTGTTTCTTGATAAGAAGCGGCAGCAAGGAATGAATCAGACAACAATGGAACTTGTTTTGCACCTTTAATAACAACTTTACCATATGCTGGCTTCAAACCTTTTGAAAGTAGAATAGCATTTTCTTCTTGATAGTCAAAAATATCTACAATATCACCAGCGAAGAATTTAGAATCTCCGGGTTCAGAAATTACTAATTTAGACATCATTTGACGAATTATAATTTCAATATACTTGTCGCTAATTGCAATACCTTGAATACGATAAATTCTTTGAATTTCTTTTAATAAATAATTTTGAACAGTAATTACGTCTGTTAAAGCTAATAATTCTTTTAAGATAATTGGTCCTTCAGAAATCTTTTGACCAATTCTTAATTCATCGCCTTCTTGAACACGTAATTTTTTGGTTGTGTTAACAAAAATTTTGTGAGAAACTGTTGTGTTTGAAGATCCTTTGCTTTCAATTGTTACTAAATAGTTTGTATTGTCTTTTTCATCAACTTCAATCTTTGTAACAATACCTTTATATGGTGAAATAACAGCAGGACGTCCTCATGGTTGTTCGTGGGCATCAATTAATTCAATCAATCTAGTGAAACCACCAGTAATATCTTCAACCCCAGCAACACCACCAGTGTGGAATGTACGCATAGTTAATTGAGTTCCTGGTTCCCCAATAGATTGAGCTGCAATAATACCTACTGCTTCACCGATATTAACAATTCTATTTGTTGCAAGGTCTTTACCAAAACACATCTTACATACACCGTTACGTGTATTGCAGCCTAAGATTGATCTAATTTCGACTTCTTCAATACCACTTGCTATTATTCGTTGAGCAATTTTTTCAGTAATTAATTCATTTCTAGAACAAATTTCTATACCCGCACTTGTAACAACAGGTTTGTTTGCAAATCTTCCAACAATTCTTTCTTTCAAAGGAACAATAATTTGTTTAGTTTTTGTATCAACTATGTTTCTTGCTTTATAACCATAGTCAGTACCACAGTTTTCTTCTCTAACAACAATATTTTGAGCAACATCAACTAAACGTCTTGTTAAATAACCAGATTTTGCAGTGTTCAAAGCAGTATCGGTTAAACCTTTTCTAGCACCGTGAGTTGATGAATAGAATTCAAATGCAGTAAGTCCTTCAATAAATGAAGACTTAACAGGAACTTCAACTGTTGATCTAACAACACGGTCGTTTTTAGCATCGGCCTTAGTAGTTTTAGTATTGTTAGCCATTAATCCACGCATACCTGCTAATTGAACATAGTTAGAAATGTTACCACGAGCACCTGAGTTAATTATGGTAATAACTGGGTTTTGAGGATTTTCTTTAATTATTTTTTGTAGGTCTTCTTGAATTAAATCCTTAACTTCTGCTCATTTTTTAATAGTTAAAGAGTAACGATCATCATCAGTTATTCAACCTTGTTCATAATAGTGTTTTAATTTAGCAATATATTCATCACCTTGAGCAATATATTCTTGCTTTTTATTAGTTTCTAGAATATCACTAAACGAAATAGAAATTCCAGAATATGTTGAATAGTCAAAACCTAATTGTTTAATACTATCCAATATTGAAGCAACAATGTTTGTATATCTAAATCATACAGTGTCTAGAATTTCTGCTTTTTCAGCTGGACTAAGTGGAACTTCTTGTCCATTTGCCCTAGCAGGATTTAACATTTCAATCTTTTGCTTTTCTTGACTTGTAAATTGAGCTAGCAACATTGCATGTTCATAATCAATTTTTCTATTTTCATAATTTTTAACATGCAAGAATTGAATTATTGAATCATTAGCATCACTTGATTCAGTTAAATTATTAATAACTTCTGCAATATCAGACATTGAAATTTGAGCAACATATTTATCAAAAACATAACGTATTATTTTAGAAATATTCTTTTTGTTAAATGCTTCTTCAATAGGCATATTTTTGATATATTCAGGAATATTAGTACCTGCTTGAACGACATATTTTGCATTGAATGTTTGATTATAATCTTCCAATGAATTTGCCTTGGCAACTTTATTGTCGTAAATAAATGGAAATGTTTTAGGGAATATATTATTAAAAATAAATTTACCTACTGTTGAAATTATATATTGAGGACCATCAAATGGGAAAATAAGTTTTGGAGTTTTAACTTCTGAAGCAGGTAATGCTATTCTAGTGTGCAATGTAACTACTTTATTTTCTAATGCTCTAAGCATGTGTTCATAGTTATCAAAAACACGTCCTTCACCTTTAGCTCCTGGTTCTTCAATAGTTAGATAATAAATACCTAAAACCATATCTTGAGAAGGGTTAATAATAGGTTCACCATCTTTAGGTCCTAGAATGTTTTTATTAGCCAACATTAATTCACGACTTTCAAGCAATGCTTGTTCAGAAACAGGAACGTGAACAGCCATTTGGTCACCATCGAAGTCAGCATTAAATGGAGTACATACTAATGGGTGTAATCTAATAGCCTTGCCTCTTACTAAAACAGGTTCAAAGGCTTGAATAGATAAACGGTGTAATGTAGGTGCACGGTTTAATAATACTAGTCTGCCCTTAATCGCTTCAGCAACATGAGGTCAAATTACTGGGTTTTGGTCTTCAATAATTTTTTTAGCACTTTTAATTGTTGTAGCTACTTCTTTTTCAATTAGCCTTTCAATTATTCATGGTTCAAATAATTTAGCAGCCATTTCTCTTGGTATACCACATTGGTGCATTTTTAATGAAGGCCCTACGGCAATAACTGAACGTCCAGAATAGTCAACACGTTTTCCTAGTAAATTTTGTCTAAAACGACCTTTTTTACCAGTTAATGCATCTGAAATTGATTTAAATGGACGATTGTCTTTTGATAAAACAGGGTTAGGTGTTCTTCTTTGGTTATCAATTAAAGCATCTACTGCTTCTTGAATCATTCTTAGTTCGTTTTGAATAACCAATGTAGGGGCATCTTTTTCTTGTCATTGTTGCAATCTATTGTTTCTAATAATTACTCTACGATACAATTCATTAATATCGCTAGTTGAGTGTCTACCACCATCTAATTGAATTAAAGGTCTTAAATCTGCTGGAATAACAGGCAAATTATAAATTAACATGCTAGTTGGATCTTGTTTAGATTCAATAAATGCATTAATTACTTGCAAACGTTTATAAAGTTTTTCTCTTGTTTGTTTTGCAGTTGTGGCAAAACGTTCAGGATTATTAATTTCTTCTGCATTTAAACGATTAATTTCATCAGTTACTTTTTGCTTTTCTTCAGCTAAATTTATGTTTTTTAATAAATATTCAATAGCTAAAGCACCAGTGCCTATTTTAGCGTCTGAATAATGTTCAATTACTTCGTTTAATTCATAAAAATCAATTCCATATTCTTGACCGATTTTTGATGTTGCTTTTTCAATTAAAGAATCTAGGGTTTCTTTAATATCATCATATTCCTCTGTACCTTCTTGAAATCTTTCAAGCAATTCTTCTAGAGCATTGCGGTAAATAATTGCTGCTTCATTAATTTCAATAATTTGATTTTTAGGTAATGATTTTAAGCCACCATCTTCTAAAACAATATGATTTTTGTAATAAATTAAATTTTCTAGTTCTTCACGTTTAACCACGTCAGAACCATTTGAAGATTTTAAACCAAGCAATTTATAAATAATTGAGTGATCAACTTTGAAGTATCAAAAGTGAACAACTGGACTATTTAGTTTTATATGTCCCATATGGGTTCTACGTGCTATTTTAGGCAATATTTGAACATGTTCTTGTTTACATAATTCAGTTCTTGTACAATAGCTTCCTTCATTAACTTTTTTGTATTTGAAACCACATACAGGGCAACGATAATCAATCATTGGTCCAAAAATTATTTCATCAAATAATCCACCACGTTCTGGCTTATATGATTTATAGTTAATAGTTTCTGGCTTAGTAACTTCACCATGAGACCAAGTTTCAACATCTTGAGGAGTTGCTAATGCTAATGAAATTTTTGAAATTTTTTCTTCATCTAATGATTCGTATTTTGAAATTTTTTTCATATTATTCTAAACTCCCTTCAAATTCTTGGAAATATTTTGTGTCTACTCTTTGATCTTCTTCTGAATCATCTACGACATTATCAGATTTTTGATGTACTTCTAATTTAATTCCTAAACCACGTAATTCATATGCTAAAACATTAAATGATTCAGGTGTTCCTGGTTTAGGCAATCTTGCATCTCTAGCAAGAGAGTTATATAGTTTATTACGTCCAACAATATCATCAGATTTATATGTTAATAATTCTTGTAGAACATTTGAAGCTCCATATGATTCAATAGCCCATGTTTCCATTTCACCAAATCTTTGACCACCATTTTGACTCTTTCCACCAAGTGGTTGTTGGGTAATCAATGAATAAGGTCCTACTGAACGTGCGTGCATTTTGTCATCAACCATGTGATACAACTTCAAATAGTACATAATTCCTACAGAAATTGGGTGATCAAATGGTTCTCCAGTGGCCCCATCGTATACTATTGATTTTCCTGTTACATCAATATTTGCTTCTTCAAGAATATCACGAATTTGGTTGTGTGAAATTCCATCAAATATTGGTGTAACAAATTTTGTATTTAATTTTTTAGCAGCAAGTCCTAAGTGCAATTCTAGAACTTGACCAATGTTCATACGTGAAGGGACACCTTGAGGGTTTAAAACAATATCTAATGGTGTTCCATCTGCTAAATAAGGCATTTCTTCAACAGGCAATACTATTGAAACAACACCTTTATTTCCGTGACGACCAGCCATCTTGTCGCCGACTTGAATTTTACGTTTTTGAGCAATTGATACCTTAACTATTTTTTCAATGCCATCTTCTAATTGGTCGCCAAATTCTCTACTTAGAATATCAACAGCAACAACGGTTCCATTGTGGCCATGTTTTACTTTTAGAGAAGTATCTTTTCTTGAGGCAGTTTTTTGACCAAAAATTGCAGCCATTAATTTTTCTTCAGGAGTTGGATTTTCTTCACCTTTTGGAGAAGTTCTTCCGACTAAAATATCACCTGCTGTTACTTCTGAACCAACTCTAACAATTCCATTTTCACCTAAGAAACGTTTAGAATAGTTTGAAACGTTAGGAATATCTGCAGTTAAAATATCTTCGCCAGCCTTTGAATGTCTAAATTGAATTGATTGTTCTTCAATGTGAATTGATGTATAAACATCGTCCTTAACCAATCTTTCAGATAAAACAACGGCATCTTCATAGTTATAACCATGTCAAGTAGTAAAACCTACTAGAACATTTTTTCCTAATGCTAATTCTCCATCTTTCATTGAAGGACCATCAACTAATAAATCACCTGCATTAATTTGTTGGCCAACTTTAACCAAAGGTACTTGGTGAATTAATGTACCTTGGTTACTTCTTTCAAATGTTCTTAAATGATAAACATCACTTTTTGAACTATTTTCAGGAGTTACTTTAATAACGTTAGCATCTACATAGTTAACAACCCCTGATCTGGTTGCTCTAATATTGGTTGATGAATATTTAGCAATATCAGCTTCAACTCCTGTAGCAACTAATGGAGCTTCTGGGAATAACACTGGAACAGCTTGACGTTGCATGTTAGAACCCATCAATGCACGGTTAGCATCATCGTTTTCCAAAAATGGTATAGCCGAAGCAGCTATTGAGGTCATTTGACGGTTTGAGACACCAATAAAATCTACATCTTCAGCATTTACTTCTAAATATTCATTGTCACGTCTTACCATTACTTTTTCATCAACAATTACATTACCTTCAATGTGAACAGTTGATTGTGCAAATGTATATCCTGTTTCTTCAATTGCAGTAATGTAAACTGGTTCTGAAAAATCTACAACTTTTTTATTTACTCTGAAATATGGTGATTGAATAAAACCATATTTATCAATTTTAGCAAATGATGCTAAATTTAAAATTAAACCAATGTTTGGTCCTTCGGGTGTTTCAATAGGACAAATTCTTCCATAATGGGTAGGGTGAACGTCACGAACTTCAAATTGTGCAGTATCACGGTTCAACCCTCTTGGTCCTAGGGAGGTTATTCTTCTCTTATTTGATATTTCAGCAAGAGGATTACATTGATCCATAAATTGAGAAAGTTTTGATGTGTTAAAGAACGATTTAAATTGATTAAATATTGGTTTATTGTTTGTAACGTTCTTAGCAGTAATCTTATCAATATCTTTTGCCGAGATTCTTTCTCTTGTATTTTTTTCTAATTTTAATAGCCCTATTCTAAATTGATTTTGTAATAATTCGCCTATAGTTACAATTCTTTTATTAACTAATGAGTCAGGATCATCATCAACCCCAACCTTTGAAAGTAAATTAAAATAATACGAAATCGTTGCAATTAAATCAGGAATCAACAAGAAATTTTCTGTTGATGTAGGATCATTACCTAAAACCAAAACTTTTTGGTCATGCAACATATCATTTTCAGTTGGATATATTCAAATGCTTGTTACAAATATTCTGCTTGCTAGTTGTTCATTTCCTTCAACTTCAAGTTGAGATCCATATGCATTTTCATCAATATCTGGCAATTTTCACATAGGAATAATTCCTTGTTTAAATTCATCATGAATCTTTCTTGCGATATTTCAAGTAATGAACATTCCTTTTTCATAAAGAAGTTCACCTTCACTAGAAAAAATATCTTCTGCTAAATAAGAATTAGCAATTCTTTCAATAATGTTTAATTTTCTATTTAAAGTGAAACGTCCTGTTTCAGTTAAACTATATCTTTTTTCATTAAACAAAGTTGTAGGAATTAAATTTCTAGCAGAATCAGCTGTCATACGATCGCCTTTTCTAATGATTCTATAAATTGCTTCTTGTGCTTCCCTTGTAACTTCTTCTGCATTAATTCCAGTGATTTTATCACGTTTAAATGTTTCTTCTAATTCTGGAATGTTTTCGCCAAACATTCTTCTAATACCTGATTCACTAAATCCTAATGCTTTTAAGAAAGTTACAACTAAAAATGATTTATTTTTATCGATATGTATTTTTACAGTATCTGGATTTTGAGAAGTTACTTTATGATAAATTTCTACTCATGAACCAATTTGAGGAATAATTTCTACTTTATTAAACAAGTCGTTGGCTTGTTTATTTCTAACATTAATTCCAAAATAAGCCCCGGTAGATCTAATTAATTGCGAAACAATAACTTTTTCTGAACCATTGATAATAAATGATCCACCATAAGTCATTATAGGAATTTCAGCAAATACTACTTCTGAATCTTCAACTTCACCTGTTTCGATATTGATTTGTCTTAGGTGTGCTTTTAACTTTCCTTGATATGATGAACCTTTTTGTTTAGCTTCTTTAATGCTTTCATATTCAGTTTTTTTAGGATATTCAAAATGTGCTGAATTATGAATGTATTCAATTCTAACTTTTCCATGTGCTTCAATTGGATAGATATTTCTTAATTCTTCTTCAATTCCAACTGTTAGAAATTTTTCAACTGATTCACGTTGCATTTCAAGAAAGTCAGGAGTTTCGAATTTCTTTTGACTAATTGAATAGTCACGTCTTAGGGTAATAGGTCCAAATTTTCTAATTTCGTATTTATCCTTATTCACCATGCTTTACCTCAATTTTTATTAAATTTTGTTTATATTTTTATTTTTTGCTGTATATACGCACACGTGCATAATATACGACAAAAAACACAAAAAAGGCTTTGTGTATTAAAAGCCTCACTTGTGTTTTTTGTAATTAGTCTAAAGTAACTTCGGCTTTAGCTTCTGTTAATTTAGCTTTTAGTTCTTCAGCTTCTTCTTTTTTAGCATTTTCTTTAATTACAACAGGTGCTGATTCAACAAGTTTTTTAGCATCCATTAATCCAAGACCTAATAAGTCTTTAACAACTTTAATAACTGCTAGCTTGTTTCCACCATCTGATTTTAAAGTAACATTAAATACAGTTTTTTCTTCTGCTGCGTCAGCTGCTGCTGCTGGAGCTGCTGCTGCTACAACTGCTGTTGGATCAATTCCAAATTCTTCTTTTAATCCTTGAATTAATTCCATAACTTCTTTAATGTTCATTTCTTTTAATGCTGAAACAAATTCTTCTTTTGTTAATTTTGCCATGATAAATCTCCTTTAAAAATAATTATTCTGAAATTTTTCCTTGTTTTATTAATTCATTTAAACCAATTGATAATTGTTTCAATGGACTCAATAATGAATTTCCAAGAATCATAAGTGATTCTGTAAATGAAGGAAGTTTTGATATTTCGTCTAATGTTTTTTCATCAACTACTTTATTTTCATAAATTCCACTTACAATTCTTAATGCAGGGAATTTTTTCTTTAAGCCTACTAAGGTTTTTAATGCTGCTAATGAATCGCCTTTAACAAAGGCATAAGCATTTTGTAAAACTAAACTATCATTAATATTTGCATATTCTGTATTTTTTAATGCTTGTTTAACTAGTCTATTTTTGTAAACTTTTAGCAATGCATTATTTGGAGCTAATTCTTTTCTAATTTCTTGAAATGAAACAACATCCAAAGTAGCATAATTAATTACATATAATGCTTGTGATTCTTTTAAATTGTTTGAAATTTCTTCAACAACTAAAACCTTGGCATCTTTTAATGCGCTCATTATTATTCCTCCTATAAAGATTATGCAATGTACAAACAAGTATAAACATACTAGGTAACATTATTAAGCACTACGCCGTTACTGTCTTCATATATTGCGTTGAAATTATAACAAATTTTTAAAATTTTTATAATTTTTAAATAAAAAAAATAGCCATTAGGCTATTTGTTGTTCTTTGAAAACTGAATACGACTTAATTTAGATAACTATATAGAATATTACTATTAAACCAATAAATTTATTAGTACTGGTCAGCTGAATGCATTGCTGCACTTACACCTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACACCATCGGTGCGTCCACTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGATCTCTTGGGAAAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCACCACACACTGTCCTCTTCCCGGATGACGGGAACAAGTTAGAAGTTCAATGTAACAAGGGTGGTATTTCAACGGCGACTACTCTTAAACTAGCGTTCAAGCATCAACGTCTCCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAGTCTATTGTCGAGACAGTTAAGAGATAATTACTCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCACATTAATGCTTCGCTAATGCTAACACCTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAACTTACGGGATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTGTGTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAAATCATCTAATTCGCTACTCAGTTGCCCTTTTGCTATGCATCGTAACTCCCAGTTAAGTTATGCGGATTTGCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCCTTTCGGCCTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAAGCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAGAAACTTCACAACCTTTCTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTATCACCGATGTACCGACTGCATAGTAATACATGATAGTATTCGGAGTTTGATTATAGTCAGTACGGCTAGGCGCCGCCATTCCATATTCAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCACCCCTACCCACAAGTCATCCGGGCACTTTTTAGCGTACTGCGGTTCGGTCCTCCACTTAGTGTTACCTAAGTTTCAACCTGCTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTCACGTGCCCCGCCCTACTCAGGATACTGCTAGGAGATTTATACATTTCGCTTACGGGAATTTCACCCTCTATGTTTAAGCGTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTCTTTATTTTCTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTCCAGACCCAAGGCATCCACCAAAAACTCTTGCTTGTTTAATAGTTTTGTTAATTGTATGACCTATTGTTTGTCTTATAAAATATAAATATTCTATGGTTGATCTAAACTTTGAAATATTAATTAATATATCAATGTCGATATTCAGTTTTCAAAGAACCGAGAGATAAATCTCTCAAAACTGGATACAAATATTTTTTTTCCATAACGTAGGTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTCACCCCAGTCACCAGTCCTACCTTAGGCGGTCGCCTCCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCAGTTACCCAGGCAGTATCTCTAGAGTCCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGACTTGACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCACTCCGATAACCTCCACTATATCTCTATAGCTTTGCGAAGGATGTCAAGAGTGGGTAAGGTTTTCCGTGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGCCCCGGGATTTAACTCCAGACTTAACAAACAGCCTACGAACGCTTTACGCCCAATAATTCCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCATCGCTTTCTGACAAGGTACCGTCAGTCTGCAATCATTTCCTATTGCAAATGTTCTTCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGTCTCTCGACCCGGCTAAACATCATAGTCTTGGTGGGCCATTACCTCACCAACTAACTAATGTTCCGCACCCTCATCTTTTAGTGGCGCCTTACAGCGCCTTTCACAACGGAACCATGCGGTTCCATGCGTATCCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATTAAGCACGTGTTACTCACCCATTCGCCGCTAGGTTATTGCTAACCTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTTATAAAAAATTTGAATTGGTTGATTATAAATAAAAAAATAAATTGACGTTAATGGTATTCGTATCCAGTTTTCAAAGAACTATCTCGTTGCCATTTTGTAATTGGCACGTTTATAATTATACATATTTTTTTGTTTTTTTATAAAAAAATAAATTTATTTTATTGTTTGTTTTTTTATAAAGTTTTTAGCAATCAAAATTGCTTGATTTATTATATAACTTTAATTAATTTTTTTGTAAAAAATAGATATTTTTTTATTTGCTTATATATTTTTTTAAAAAATGCCTTAATAATTATTCATTAATAATTTAATTATTTATTTTTTTAAATTGAAGAGTTTGATAAATAAAAATTCTCTCGAATAAATCAAGAGAATTTTTTATTATTTAATATTATTTAATTTTATCGTTAATTGCTTCTTTAATAACTTTTCTTCCTCTATAAAAACCGCAGTTTTCACATGCTTGGTGAGGAGTAATTAAATTCTTACATTGTGAACATTCAACTAATGTTGGAGCCACTAGAGCGTGGTGTGATCTTCTTAAATGTTTTCTTTGTTTTGAAGTTTTTCTTTTTGGTACTACTGCCATAATTTTCTCCTTTAAATAATCAAATTATACCAATTCGATTATTGCCATTTTAGCATTATCACCAGCACGATTGGCTAATTTTGTAATTCTTGTATATCCACCATTACGTTCAACGTATTTTGGTGCCACAACATCAAATAAATATTGTAATGAATTTTTATTTTTGTTTTCTGTTGCAATTAATCTTAAATATTTGGCTGCTTGTCTACGCGAAGCTAGGGTATTTTTCTTACCTAGAGTAATCATTTTTTCTGCATTTGAACGAATTCTTTTAGCACGTTCTAATGTGGTTGTTACTTTGCCATTAATCAATAAATCTGTTACAAGTGATCTTTCAACGTGGTCTCATCACTCAGTATTTCTACGAAATAGTTGTTTTGGATTTGCCATTGTTATTCTCCTTCTTCGTTAGCAGCATATTCTTTGTTAGAATCTTCATTATTTAATTGACTATCAAGTCATTCTCTACGACGGTCAATAATATCTTGTACTGATTTTTTACCCAAATTTTTAATATTCGAAAGTTCTTCATCGCTTAATTTCATTATTTCATCAACATTATTAAATCCAGCACGTCTTAAGGCATTCAATGAACGAATTGTCAAATTCAATTTATCAATAGAAACTGAAACTTTTGGAGTTTTTTCATGTTTTTCTTTTTGATCATCGAATAAATCAATTGTTCCTAATGCGTCAATGTTTCCAATGATTTGGAAATGAGCAACTATAATCTTTGCTGCTTCTTCCATAACGTTTTTAGCTTCAACAGTTCCATCGGTTTCAACTTTAATTTTTAATCTTTCTTCAATTATGTTAGAAGTAGAATTTAATTCTTCAAATAAAATCGCAACTTTTTTAATAGGACTGAAGTCACTATCCATTGCCAAAAATTGACCATTTTTGATTTTTGATTCTAATTTTGGACCATATTCTAGAATGGTTTTTTTATTGTCTTCAAATGAAACATAGCCTCTACCGGTTCTTAAATATAATTCAAATTCCAATGAACCATTTTTTGATATTGTTGCTATGTATTGATCTTTATTAACAATGTCAAGTCCAGGGATAGTTTCAATATCCGAAGCATGAACTTCCCCTTCTTTTGTTGATTTAAATGAAATTTTAGCTAAATTATCTTTTTCAAATAATTCTGGGTCATATGCAAAGCGAATTTTTCTTATGTTTGCAACTAATGTAATAGCATCTTCTCTTAATCCTTCAATTGCAGTAAATTCATGTTCAACATGACTAATTTTTATTGCAAATGGAGCAACAGAAGTAATTGAAGAAAGTAATGTTCTTCTTATTACAGTTCCTATTGTGTTACCATAACCACGCATTAGTGGTTCAATAACAAATGTTGTATTAAAATCTGATACTTTTTCTGCTACTAATTCTTTATATGTAATTTTTTGTATTTTCTTCATATTTATTCCTTAAAAAACAAAATAACAAATTATCCACGTTTAAGAATCTTTTTAGGAGGACGAGTTCCATTGTGAGGAGTAGGTGTTACATCCTTCACTTCTTTAACACTTAAACCACTTGTTTCTATTGATTTTCTAGCAGTTGATTTTCCAGGACCTACACCTTTAACTAAAATTGTAACTTCCTTTAAACCAAATTCTTTTGCTTTTTCAACTGCAGCAGCAGCAGCAAGTCCTGCTGCATATGGGGTTTTCTTCTTTGTGCCTTTGTATCCTATTGCACCAGAAGAACTTCAAGCAAAAACATTTCCTTTTAGATCTGAGAAAGAAACAATTGTGTTTTGGTAAGTTGAGTGAATATGTGCAATACCTTGAGTAATAACTTTTTTATTTTTCTTTGCCATTATTATTTACCTTTCTTTCCTGCTATTGTTTTTCTTGGACCTTTTCTTGTTCTTGCATTTTTCTTTGTAGATTGACCACGTACAGGTAATCCCTTACGGTGTCTAATACCACGATATGATTTAATTTCCATTAATCTTTTAATGTTTAAATTTATTTCTCTTCTTAAATCACCTTCAGTGGTGTATTTTTTAGCTTCATCTCTGATTCTAGTTAATTCTTCTTCGCTAAGATCTTTAACTCTTTTTTCGCCGTCAACATTAGCATCTTTTAAAATTTCTTTTGCTAGTGAAACGCCAATACCAAAGATGTAAGTAAGTGAAATACGAACTTTTTTATTATTAGGAATTTCAATGTTTAAAACTCTGGCCATGTTTTATCCTTGTCTTTGTTTATGTTTTGGGTTAATGCAAATTACGCGATTAACACCATGTCTTTTAATTATCTTGCAATTATTGCAAATACGCTTAATAGAAGCTCTGACTTTCATATTTCTCCTTATTATTTGTGTCTAAAAACAATTCTACCTCTGCTCATATCATATGGACTAAGTTCAACATCAACTAAATCCCCAGGGATCATTTTGATATTGTGAAGTGACATTTTACCTGAAATAGAAGCTTTAATAGTAAGTCCATTTTCTAATAAAACATCATAGTCTCTTGATGTGTGCATTTTAGTAATTTTGCCTGACATTTTGATTGCGTCTTTTGCCATTAAATATTGTCTCCTGAGAGTATATATGCTTGGTCATTGTCAATTAAAACTGTTTGTTCATAATGAGCAGTGTTATAACCAAGAGGATCATAAACTGTTCATCCATTTCCTTTGACCAATACATTTTTAGATTTTTGTAAAATCATTGGTTCAATGCAAATTACCATACCATTTCTAATTAGCTGTCCAGTTCCTGGATAGCCATCGTTATAAACATATGGATCTTCATGCAACTTTCTGCCTATTCCGTGACCTGTATATTCATCTGGAGTAAAATATCCTCTATCACGAATTAAATTTCCTATGGCTGCAGAAATATCACCAATGTGTTTGCCAACTTTAATTGCATTAAAGCCGGCATAAAAAGCGTCTTTTGCGCAGGCAATTAATTTTTTGTCTGCTTCAGTTATGTTGCCAACTCCTTTTGTAAATGCTGAATCAGAATAATAGCCATCATAAATTGCTCCAGTGTCAATTTTGACTATATCTCCATCCTTTACAACATAATCACTAGGAATTCCGTGAATTAATTCATTATTAACAGAAATGCAACACGTATTAGGGAAACCATATTGTCCTAAGAATGCTGGTTTTCCACCCCTTTTTCTTATTTCTTTAAAAGCTACGCTATCAATTTCTTTTAAAGAAACGCCTGGTCTTATAAAGTCGTATAAAACAGTTTTCACTTCTGCCAGGATCTTACAAGCTTTTTTGATCTTTTCAATTTCAAATTGATTTTTTATTATGACCATTTTAAAGCCTCTTCTACATCATTATATACTTTATCAATTGTTTGATAACTATTTATCTTTAAAAGTATACCAAGTTCTTGATAAAAATTGATCAAACATTTAGTTTGTTCATTATATATTGATAAACGTTTCTTTATTACTTCTGGTTCGTCGTCTGGTCTTTTTGTTACTTTTTCGTGGCATTTAATACACAAATCACCAGGTAATGGTGGAAATGATTGCAAATGATAAATGGTTTTACAATTTAAACAAATTCTACGTTTTGAAAGACGGTCAATAATTTGTTTATCAGTAATTTCTAGTAAAATTACTTTATCAATTTTGATTCCAACATTTGCTAATGAAAGTAAAAACTTTGCTTGAGCTAATGTACGGGGATATCCGTCTAATATGAAGTGTTTATTTTCTCTTACTAATTCAGTTAATTTTTTTTCAACTAGTTGATTTGTTATACAATCATCAACATATGCTCCCGAATCTAAAATAGATTTTATTTTTAAGCCAAGGGGAGTTTGATTTTTAATTTCTTGTCTAAACATATCTCCAGTTGAAATCTGAACATAATCATTATTTTGCACCATTTTTGTTGCAATAGAGCCTTTACCAGCTCCTGGTGCGCCAAGCATAATTATATTAGGAAATTTTTTTGCGTGTTCGTTGTACACTTCTTGAGATTTTTCTATCATATTAAATCTTCTTCTTCCTCTCCATACATTTCTTTTAATTCTCTTATCATTTTTCTCTTTCTTGATAATTCTTGTGACTTGTAGCGGGCTTTAATTTGTTGAACAGTTTCATAAGCCGTTGAAACTAGTATCATTATACTTGTACCACCAAATGCTATTGAGGCTGGCATGCCTAGCATTTGTTGAATAAATTGTAAAGCGCCTAGCAGCAATAAATATCCTGAAGAAAAGAATGATAAACGTAAAATTACATTCAATAAATAATCTTCTGTTTGTTCTCCGGGTCTAACGCCAGGAATAAAAGTACTGTTTTTAGCAAAATCTTCACTAATTTTATCAATTCTTGATTGTTGGATTCCCATTAATATTGTTAATCCAAATGTAATTAAAATAAATAGAAAGAATCCAAGTGGTTGAGTAAATTGCAAATTGTTGTAAACTCATTGATAATACTTGCTTGTATTTGGATCAAATAAGTTTGCAATCATTGTTGGCACACTCAATATCATCATTGAGAAGATAACTGACATGATACCAGCAGGATTTGCTTTAATAGGTAAATAACTCAATTCTTTTTCGTTTTTAGAAAGACCCGCACCAACTTGTTGAATTGGAATATGTCTTTCTGCAATTGTAAATATTACAACAATAAATATTACAAGTAAATAGCAAAAGATATATACTGCGTAATTTATTGCTTCTTGTAAAACTAAATTATTTTGTTGAGCTGATGGAACATAAAATTTAAATGCACTCTTGAATGAAGGAATTAAAGCACTTGCTATACCAACAAAGATTATTAAACTCGTTCCATTGCCAACGCCTCTATTAGTTATTTGTTCTGATAAGAACAATGAAAAGAATGAGCCAGCTACTAATATTGCAGGCAAAATGAAATATTGATATGCTACCATTCCACTTTGTCCAAATAATTTTTCTAATTTTGGTGAAAAAGTAATTCCAAATCCTTGACGAGGATTAACTAATGCTTTTGTTATCAAATATGCTTGAACTATTGCAAAAACAAAAGTAACTCCGTAAGTAATGAAATTTATTTTAATCCGGCCTTGAGGACCAGATTGACTTAAACGGTGAATGGCCGGGAATGCTTTAGTTTGCAATATCATCATAACTAAACTAGATGATATAAACGGCCCTATTCCTAGCGCGACAACAGAGAATTGTCTTAATCCGCCGCCACCTACTAAATTCAAAATCCCAAAGAAATCTCCTTGGGTTAATGAATCAGGGTTTGCTAAATTGACACCTGGAATTGTAATTGTTCCAGTGGCTAGAAAAAGAGTAATAATGAATAAGGTGAAAAGAATCTTTTTAAAAAGATTATGATTTTTTCATCATTCATTTCAAGTGTTTTTTTTATCAACTAAAAACTTATCTAGTGCTAATTTGCGCTCGATCTTTTCTTGACTAATCTCTTTTTCCAACTTAGTTTTTCTAGCCATTATTTAACCTCTATTTTTCCTCCGACTTTTTCAATAGCTTCAATAGCGGATTGTGAAAGTGTTGGAATTTGTACATTAAGTTTTTTAGTTAAAGAACCCTTAGCTAAAATTTTAACTGGTAATGTTCCTTTAATCAAGTTTCTTGCAGCAAGTGTTTCGTAAGAAACTACTTCACCATCTTGATATTTTGATTGTAAATCTGCTAGATTTACTACTTGGAATTCAACGTGATTTACATTGCTAAATCCACGTTTTGGAATTCTTCTAAATAGTGGCAATTGGCCACCTTCAAATCCTAGTCTTACTTTGCTTCTTTTTGTTTGACCACTTTGTCCTCTACCGGCTTGTTTTCCTTTTCCAGCAGCATGACCACGACCAACACGGTGTTTGTCTTTTCTTGCCCCTGGAGTTGGTTTTAAATTATGTAATTGCATAATCACTCCTTATTTTGATAATAGATGATTTACATCAATATCGCGTAATTTTGCGATTTCTTCAACAGTTCTAACTTTTTTAAGTGCCTTAATAGTAGCTTGGACAATGTTTTGTTTTGTTCTTGAACCATAAGTTTTTGTTGAAATGTCGGTGTAACCTGCTAATTCAACAACAGCACGAACGGTGTTTGAAGCAATGATTCCGGCACCTTTTGGAGCTGGACGTAATTGGATTCTTGATGCTAAGAATTTATCTTGAATTTCATGAGGGATTGTTCCATTTGAAATTGGAACTGAGAAAACATTTTTTTGTGCATCTTTAACTGCTTTTTTAATTGCATCTTGGACTTCATTAGCTTTTCCGTGTCCAAATCCAACTTTTCCTTTTTTGTCACCAACAACAACATAAGCTGAGAATGAAAATCTACGTCCACCCTTAACAACAGTTGTAACTCTTGCAATGTCAATAACTTTTTCTTCGAATTGTTTTGCTTGAGCTTGATTTGCTGAATGATCTTGATTACGATTTTGGAATTGTTTTGGTTTATTTTCGCTTGATTTTAGATCTTTTGAATTTGATCTTTCTCTTTTAAAAGAAACAACCTTTGTGCCTTCTGTTTTATTTTCTTTTAATTCAGGTTTTTCTGAATTTTTAATATTTTTTTCTACTTCAGCCATTAGAATTTAATCCCTTCTTCTCTTAGAGTATCAGCAAAAGCTGCTAATTTTCCGTGGTATATGTATCCTGAACGATCAAACACTATTGATTTTACATTGATTGCCAATAATTTTTTAGCAAAATCTTTTGCAACTAATTTAACTGCATCAATATTGTTTGCTTTATTTTCTAGTATTTGTGTTGATGATGAAGCGATTGTGGTGTGCAATTCATCATTTACTGCTTGTGCATAAAATGCATGTAAAGATTTAAAAACAACAACTCTAGGAACTAATGCAGTTCCTTTTGACAATTTGTTAGTAATCTTTAAGTGTTTTACTTTACGTTTTAAATTTCTTGATAACATAATTTATAACTCCTTAGGCTACTATTTAGCAGCTGTCTTTCCTTCCTTACGTCTAATAACTTCATCTTTATACATAATACCTTTTCCAGAATATGGACTTGGACGTCTTACATCACGAATATTTGCAGCAAATTCACCAACAGCTTGTTTGTCAATTCCTGAAATAATAATATTGGTTGGTTTTGGTAATTCAATTTTTAGATTTGATGGAACTTCTAAATTTACAGCATGTGAATAACCAGCAATAATTTCAATTGTATTTCCCTTTAAGGTGGCTTTGTAACCTACACCTTTAATTTCAATTTCTTTTTTGTAACCACTTGAAACACCTATTAACATATTTTTAATGTTTGCATTTGTTGTTCCGTGTAATTGTTTTGTAGATTTTTCTTCGTTAGATCTCAATGTCTTAATTTCGTTGTTTTCAACAACTACTGAAATTAATGGTGAGAATGTATAACTCAATTCTCCTAATTTGCCTTTTACTTCGATATGGTTTTTATCTAATTTAACTTCAACATCTTTTGGTATTTCTAAAACTCTTTTTCCGATTCTAGACATAATTACTTCTTTTTAAGTTTATTATCAAATGAAGGCGATAACTTCGCCACCAACATTAGCCTTACGAGCTTCTTTATCTGTTAATAAACCTTTTGATGTTGAAATAATAGCTGTTCCATATCCACTTAAAACTCTAGGCAATTTTTCAGCAGATGAATATACTTTTAAGCCAGGTTTTGAAACTTTTTTAAGACCAGAAATTGCTGATTGATTTTGATTCATACCTTTGTATTTTAAAGTAACTTTTATTTCTTTAAAATTGGTTTTTTCGTTTTTTACAACTTCAAAATCAACAATATATCCTTCTCTTTTAATAATTTCTAAAATTTTTGCTTTCTTGTTTGAATGTGGCAATACAACTTCATGATATTTACGAGAAATTGCATTTTTAATACGTACAAACATATCTGCAACTGGATCTATAATAATAGCCATAATTATCAGCTCGCTTTCTTAACACCAGGAAGTTTTCCTTCATGTGCTAATTTTCTAAAACAAATTCTACAAATTTTATATTTACGCAATACAGCGTGAACACGGCCACAAAGTTGGCAACGAGTATATTTTCTAACCTTAAATTTAGGTTGGCGTTCGGCTTTAACCTTTAATGATGTTCTTGCCATAATATCTCCTAATTATTGCCCTTTGCAAAAGGGATTCCTAAGTGTTTTAATAATGCAAATGCTTCTTTATTGCTTTTTGCAGTTGTTACAAGAATAACATCCATACCTTTTAATTTACGGATTTTATCAAATGAAATTTCAGGGAAAATAATTTCTTCTTTAATGCCGATAGCATAATTTCCACGGCCGTCAAAGCTTTTTAGTGATGTTCCATTGAAGTCACGTACACGAGGTAAAGCAACATTAATTAATTTTGTTAAAAATGATCACATCTTTTCTCTTCTTAGTGTAACTTTCCCTCCCATTGGCATTCCTTCACGTAATTTTCATGAAGCCAATGATTTCTTAGCAGTTGTTTGATATGGTTTTTGACCTGTAATTTGAGCAAGTTCAACCATAACTTCTTCGATTGCCTTTGAGTTTGAAACTTCGTTACCGGCAGTCATGTTAATAACGATTTTTTCTAGTTTAGGAGCTTGCATTACACTGGTGTAACCAAATTCTTTAACTAGCGCGAAACGAATTTCGTCTAAATATTTTTGTTCTAATGCTAATTTAATTTGTTTTGCCATATATTTGCTCCTTTCTAGATAGTCTTGTTAATTTTCTTCATCACTCTTTGTTTTTTGTTGGTTTTAGGATCAACTTTTCAACCAATTTTTGAACCGATTGATTTTTGACCTTGAGCACCTTTTTTAACCAAATAAGCAACGTTTGAAATATGAATTGGGTATTCTTTCTTTTCAATTTTTCCATCATTATTTTCTTGTGATGGTTTATGGTGTTTAGTTTTAATGTTTACGCCTTTTAAAACAACAGTTTGTTCTTTTTGATTTGTTGATAAAACAGCAGCAAATTTTCCTTTGTCTTTACCAGCAATAATTAATACCATGTCATTTTTTCTAATTTTAGCTTGAGCCATATTATAAAACCTCCGGAGCTAATGAAACAATCTTTAAAAAACCTTTTTCACGTAATTCTCTTGCTACTGGACCAAATACCCGAGTTCCTCTTAATGAGTTATCTTCTTTAATTAATACAACAGCATTGTCATCAAATCTTATATGAGAACCATTTTCACGCATTAAACCACGTTTTGTTCTAACTATAACTGCTTTAACAACTTGGCCTTCTTTTACAATTCCGTTTGGAATAGCTTTTTTAATAGTTACAACAACAATATCACCAATGTTGGTTGATCTAACAATTGATCCACCTAGGTTTTTAATAATCATAACTTCTTTTGCGCCTGAATTGTCAGCAACGTTACATCTTGAAAATTCACTAAGCATTTTCTGCTCCTTCAATTGCGTGTGATTTGATTTCTACTAGTCTAAAGTGTTTGGTTTTTGATAAAGGTCTTGTTTCTTGAATTTTAACAATGTCACCAACTTTAGCTAAATTTTGTTCATCATGAACTGCGAATTTCTTTGATTTTTTAAAACGTTTTGAATAAAGAGGGTGTTTTTCGTAAATTTCTACAACAACAGTAATTGTTTTTTGGTTTTTAGTTGATACAACTGTTCCAACTAAAGTTTTACGACGGTTTTCTCTAACTGTAGTTTCCATTATTTATTCCCTTCTAAAGTTTTTGCTTTAATTGCTGTTAAAGTTCTTGCGATATCTTTTTTAACTAATTGGATTTTGTGGGTTTCGTCTAATTGTCTTGTAGCATTTTTGAAACGTAATGAGAATAGTTCTGCTCTTAAATCAGCAACCAACTTATTTAGTTCGTCAAGTGATTTTTCTTTTAATTCTGCAAATGTCATAGTAATTATTCTCCTTCAACTTCATTAGTTTTAGTTTCTAAAGATTGTTCCTTTGAAATAACTTTTCAAGTTACAGGTAGTTTGTGACCACCTAATCTCAATGCTTCCTTCATGGTATCAACTGTATTGCCTAAAACTTCAAACATTACTGTTCCTTCTTTTACGACTGCAAATCATTGATCTGGGCTACCCTTACCAGATCCCATACGAACACCAATAGGTTTACTAGTTTTTGACATGTGAGGGAATATTTTGATAATAACCTTTCCTTCACGTCCCATATGTCTAGTAATAGCAATACGGGCTGCTTCGATTTGTCTTGCACTAATTCATGCTGATGAAGTTGATTTTAAACCAAATTCGCCAAATGCAACTGTATTATTACGGTGCGCAACAACTTTGTCGTGGCGGATACGGAACATTTTTCTATGTTTAGTTCTTTTTGGTTGAAGCATGTCTTTCACCGCCTTTTTTAAATTCTCTTTTACGTGGAGCTTCTTTTACTTCTGCTTCACTTGTTTTAGGTGATAAAATTTCGCCTAATGAAACTCATACTTTAACTCCTAAGATACCATATGTTGTTAAGGCTGTTGTTACAGCATAATCAACATCTTGTCTTAAAGTATGTAATTTCATTTCACCTTCGGTATATCCTTCTGTTCTAGCCATATCAACGCCATTTAGACGTCCAGCTACTGAAGTTTTAATACCTTTTGCGCCAGCTTTTAATGCTGCTTTAATTGCAAATTTTTGTGCTAGTCTAAAACTACCACGATTTTCTAATTTAATTGCAATTCCTTCTGCTAGCAATCTAGCATTTAAATCAGGATTTTTTAATTCAATTACTTCAATAGTAATGTCAGCTTGTTTTTTAGCAATAACCTTTTTTAATTGTGCTGTTAAGGCTTTGACATTTTCACCGTTTGTTCCAAGGAATGCTGCAGGTTTTGCAGTGTGAATATAAACAATAGTATGTCCTGCTTGATCACGTTTAATTTGTACTTTACCAATTAAATATTCACGAACTTTTTTGTCAAAGAAATCATAAATTTTTTGATCTTCTAATAAATAAGTTGCAAATTTATCTTTATCTGCATATCAAGTTGTGTTATGAGATTTTGTAATTCCATAACGAAATCCATTTGGATTAACTTTTTGACCCATTATTTATCTCCTTTTTTGTTGATTTCTTCAACATTATTGATTTCTGTTAATTCAATAAAGAAATGACTTGTTCTTTTTAAAATTGCATAAGCACGTCCACGTGAGTGAGGTTGGAATCTTTTTAGTGTAGGTCCATCATTTACTAAAATGGTAGTTACATAAAGTTTAGTTGCATCAAGTCCAGCATTATTTGTTGCATTAGCAATTGCTGAATTTAGAAGTTTTAGAATAATTTCTGACGCTTTTTTGTGTGTATGTTTTAGAATAACTATTGCTTGAGTTGCTGATTTATATCTAATTAAATCTGCTACTAATCTTGCTTTACGTGGTGAAATTCTTTGCATGCGAACTGATGCATGTGCTGAATTTTGTAAAATTTCTTTAGCCATATTCTATTTCTTTCCTTTATCTGCACCATGACCATTGAAGGTTCTTGTTGGAGCAAATTCACCTAGTTTGTGTCCTACCATATCATTTGTAACAAATACATCAATAAATGCATGTCCATTGTGAACTTGGAAGGTTAAACCGATAAATTCTGGATAAATTGTTGAGCGTCTTGATCATGTTTTAATTGGACGTTTTGGTGATTTATTTTCAATAATAGCCAAAACTTTCTTCATTAAATGATCATCAACAAAGGGAGCTTTCTTTAATGAACGAGCCATTATTTAGTTTCCTTTCTTCTTCTAATAATAAATTGATTTGATGCTTTTTTAGTTGCACGAGTTTTAACACCAAGTGCTCTTTTTCCTCATGGAGTCATAGGACTCTTTCTACCAATAGGTTGTCTACCTTCACCACCACCATGTGGGTGATCATTAGGGTTCATAGCTGAACCACGAACTGTTGGTCTAATTCCTCTTAAACGGTTACGTCCTGCCTTACCAATATTAACAAGTGAGTGTTCTTCGTTTCCTACTTCACCAACTGTTGCACGATTAATTGCTAAAACTTTTCTAACTTCGCCTGATTTTAATCTTAGAATTACATAACGACCTGATTCATCTTTACCTAAGATTTGTGCTGATGATCCAGCTGATCTAGCAATGATTCCACCTTGTCTTGGTTGTAATTCAATATTGTGAACAAATGTACCTTCAGGAATGTTGGCTAATGGTAAATGGTTACCAACTTGAATGTCAACATTATCACCTGACAAAATAACTTGTCCAATTTTGATACCTTTTGGGCTTAAAATATATCTTTTTTCACCATCTCTATAAACAACTAATGAAATATTAGCTGAACGATTAGGGTCATATTCAATAGTTTTTACAGTAGCTGGGATTCCATCTTTTTGACGTTTAAAATCAATGATACGATAGAATCTTTTCAATCTACCACCTTGATGACGGGTAGTGATTTTACCTTGATTGTTTCTTCCTGCTTTTGTTGGAAGAGCAACAAGCAATGATTTTTCAGGAGCATGTCCTGTTAAATTTGCTTGATAATCAAGAGAAGACATATTACGACGGCCATTGGTATATGCTTTAAATTTTTTAATAGCCATGTTGTTTTCTCCGTTCTTAAAAATATTGTTATCTTTTAAGAAAATATTAATTATTTTTTTAATTTAGTTTCATCTTATATTTATGCAAGATTAACTATTTGTTTTCAGCAGCTTTTTTAGCTTCAGCTGCTTTAATTTTTTCTGCTGCTTTTAATTCTGCAGCGGTAGGTTCAGCAGGTTTTTCTTCTTTAACTTCTTTTTCTGCTGTTGCTTTTTCAGCTTCTTTGTTAGCTTTCTTTAATTCTTCAGCAAATAGAATAATTTTTGAATCTTTTGCTAAATATACAGTTGCTTTTTTAACTGAATTTTTAAAACCTGAGAAACGTCCTAATTTAGCAGGTTTTTTATCGTAATTAGTAATGTTAACTTTTAATACTTTTACATCAAAAATAAATTCAACTGCTTTTTTAACTTCGATTTTGTTTGTTCTTTTATCAACTACAAATGTATAAACGTTTTTATTTTCACGTGATAATTCACTTTTTTCTGTTAAAAGTGGGTATTTAATAACTTCATTTATATTCATTATTTAACCAATCCTTCTAAGTATTTAACATCATCTGAACTAATAACTAAAACATCTGCCGCCAATAAAGATTCAACTGATAAACTAGTTACTTTTGTTACAGTTACATTTGGTAAGTTTCTTGCTGATAAGAACACTGTTTCATTATTTGAAACTAATAAAACCTTGTTTAGACTAGCAATTTTATCTTCGTTTAATTTAATTAATAATTCACGAGTTGAAATTTTTGATAAAGAATATTCTTTTACTAAAACAGCATGGTCTTTTGCTAATAAAGTTAATGCTGATAATAAAGCGTTTCTTCTTGTTTTTTTGTTGATTTTTAGATTGTAGTTTCTTTCAGTTGTTGGGCCGAATACCTTACCACCACCAACAAATATAGGTGATCTTAGTGATCCAGCACGAGCATTACCAGTATGTTTTTGATCTCATGGTTTCTTACCACTTCCACTAACTTCTCCACGAGTTTTAGTTTTGTGAGTAGCAAGTCTTCTTGAGCCACGTTCTGATAAAATTGCATCAAAAATTGCTTGGTTATAAATTTTTTCTAAACCAAATACTGATTTAGGAAGATTTTCACTTGAGAATTCAACGTGTTCTTCATTATCTTTTTTAACTGATATTTTTGATGGTTTTTTAGGAAGGGTTTTTTCTACTAGATTTTCTTTTTCTAAGTAGTCGATTGCTTCTTCATTTTTAACTTTTTCGCCAGTTACTTTTTCTAAAATTAATTTAGCTTTTTCTAATCCAACTGATCCGCGGTAAGCGCCATCACGGTGGAATCATACTCTAGTATTGTTTTTTGATTTTTCAGAAAGTTCAATAAATTCTGTTAATGCTTCTGAAAAATTTTCAAAATTCTTGAATGATTTTTGGTTAGCCTTTTTTAAATTAAATGAAATATTACGATTTTCATCAAATTTTTCAGAAATGTAGAATTTTTCTAATGTTACATTTTTTGTTGCCATTATTTATTTTCTCCTTCTTCTCCGGCCTTAGCAATTGCTTCATCCAAAGCAGCTTTCATTTCTTTTAATGACATATCCATGTTTAATTCAAGATTGTATTTTTTAGCTTGAGTAAAGATTTCATTTTTAGCCAAAGCTTCTTTTAGATTCAATAATTTAATAACTGGTTTATTCTTAATTGATTTCTTAGCAGTTTTAATGATTAAGAATGCTCCTTTAGCACCAGGAACTGATCCCTTGATCAATAAAATATTGTTTTCTGCATCTTCTTTAACAACTTCTAGGTTTTGAACAGTTACTTGTTCAGCTCCTAAATGTCCTGGCATGGTCATACCTTTTCAAACTCTATTTCCAGAAATATCTCCTAGAGAACCGGTTTGTCTTACAGGTTGTGAACCACCACCACCACCGTGTGATTTAGGGCCAATATGTTGATTTCATCTCTTGATGGTACCCGCAAATCCTTTACCTTTAGAAATAGCTGTTACGTCAACAACTTCTCCAGCGGTAAATAATGAAGCATCTACAGTATCACCTAATGAAAATCCTGACATATCACGGATTTCTTTTACGAAGCGCTTAGGTGTTGTATTTGCTTGTTTAAAGTAATTAACTTCAGGTTTAATGATTCTTGAAGATTTTTTATCTTCTGTTGCTAATTGAAGAGCAACATAACCATTTTTTTCTGATGTTAAAACCTTAGTAACAACATTTGGTTTAACTTCTACTACTGTCACAGGTATTAATTTACCTTCTGAAGTAAATAGTTGAGTCATCCCAACTTTTCTTCCTAAGATTCCTTTCATGATTATCCTTTCAAATTTATTAATTTATTAAATATATATACTATATTTATAAATAAAAAATAATTACTGTTATTGATTAACAGTTTTTAAACTTATTTGTACACCAGCAGATAAATCTAATCTCTTAGCTTTATCAACTAATGATTCAGAAACTTTATCTAAAACGATTAAACGTTTATGAGTTCTGCTTTCAAATTGTTCACGAGATTTTTTGTTAACGTGAACAGATCTTAAGATGGTAATAATTTCTTTATGTGTTGGTAAAGGAATTGGACCACTAATAGTTGCTTTTTCAGCCTGAGCTAATAAAACTATTTTTTTAGCAGCTTCATCTACTAAGTGGGCATCAAAAGATTTAATTTTGACTTCTAGTTTCATATTTGTTCTCCTTCTTCGTAATTCGAAGTAACTCCACACAAGTTCTCAATATGCGAGATGAACAACTAAGCTCGGCATATCGTAACCTCGTGTTTCTTCGTTGTAGTTGCAAAAGAATTATAACAAAATTTTTATTTTTTAAAAAAATAATTTTTTTTGATGAAAAAAACATGCTTTTTACTTTTGATTTTATTAAAAAATTAACGATAAAAAATAATCGACTTACACTAAAAAATATAGATATTAAAAAACAATATAAATAATTAGTTTCTTATAAAAAATATTAAAAATAGTTGTGTAAGGTCAGTAAGTATATCATAAGGTCTATAAAAAGCTACGTTGGCTCTGTTTTATATCACTAAAAGAATTTGTTATTTGTAAGTATAATAGTTCTTTTTTGTTTTTAAGTTTTTATAAATAAATCAAACTATGCTATAATACATTCATATGAACCTAGAGAGATACACGTAAGTAATCGAACAGTAGCATGCTAGTTCGTGTAGCTACAATTACTCTAGGCTGGTTCGTCTTAAAAAGAGGTTTGTTCCTCTTTTTTTGTATAATTTTTATATGAGTTTAGAACTATGAAAACCATTAAAAATTACAAATCCTTTATTAAAATGACACTAGATTTAAAGTTCATCCCTTGATAATTTTTAAACATTTTTACATCTTATTTAATCTATTTATAATATAATTAGTTTAATTAGCTTGAGGCAATACTTATAAGTAATCGAAAAATAGGCAAGCTTATAAGGCTTATGTGTTAAATGTCTATTGATACCCCTCTAAGCTCTAGTTCGTCTTAAAAAGAGGTTTGTTCCTCTTTTTTTGTATCCTTTACTAGAAAATATTTTTAAATTAACAATTGATGGCAAAGTTTTAAATAATGTAAAAATTTAAGCTCATCCTGATAACCTTTGAGAATGTTACAAAATTAAATCGTTTTACGCTATGATGTATTCATATAAACCTAGATAGGTGCATATAAGTAATCGAACAGTAGCAAGCTTATATGTAGCTACAATTACTCTAGGTTGGTTCATCTTAAAAAGAGGTTGTTCCTCTTGTAAAAATGAAAATTCAAGTGCCCCGAACTAAATTTTATTTCAAATTTGTATTGCACTTGAATTTTAATTTTGACAACCATCAATTTGATTTTCAAATTGCTAGTTTTCTATTTATTTTTTTAATTTTTAAAATTAATCCTATTAATCAGCATCCGAAAGATCTAAGTAGATATATCCTTCCGATTCTTTACCCATTAAATTGTTTTTAATCATATCTGGACAAAGAATTTGTATATAAATACAAGGCATATTCTTTTCTTCACCTTTTTTTAAAAATTCAATTAAAAGTTTGTGACCTCAAGGCATAAGATTTTCAGGAATTTTTATATCAAAATATTTTTTAATGTCATTAGCTTCCATAAAATATGTATAAGCAATTCTTTCTTGACTTTTTAGCATTTCAATAGTGTCGTATTTTTTAGTTTCATCAATTGCCTTATTAACAATTTCAATTAAGGCTTTGGCTCCTTTGTTGGCTACAAATTTTTTGCCTTCTTTGGAAAGTTCAAATGAAAATTGTGTTGTTATTTCTGAAATTTGAGAAGCGGATAATAGCTTTCTTTGAAACATTCTATGAAGTCTATCGGGTTGTACAGGATCTGTATCATCAGTATTTTCGTTATTTGTATCTTTATTGTCATCTTTCTTGTCGTTAGTATCGGCGGTACCGGTATTTGTGCTGTCTGTATTTTTGTCATTAGTATCTGTAGTGCCAACACTAGAATACCTATATTCTTATTATCAGTATTAGTGGTACCAGTATTTGTACTATCTGTATTTTTGCCATTAGTATCTGTAGTGCCACTATTATCTATATCAATAGTGTTATTACTTGTATCTAAGGTATTATTAATTGTGGTAGTTTCCTTGCAAGATATGCTTAAAAGAGGTAATAATGACACAGCTGGAAAAATAAAACTTAATAAAATATTTTTTTTCATATTCCCTTCACCTTTTAATAACTATGATTATACAACAATACGGCAAAATATATTAGTCAAAAAAATTTTAAATTTGAGCAAAATAATGTTTATATTTCTTTACATTTAGTAAAATTTAATTACTAGATAACAATATTTTGTTTTTTAGTTTAAATTTCAAATAATAAAATAATAATAAAAATTAAATCAAGGAGAAATATGATTTCTAAAATTTATGATGATAAAAAATATCTTGAAAAAATGGATAAATGATTTAGAGCTGCTAATTATTTAGGTGTTTGTCAAATGTATTTAAGAGACAACCCTTTGTTAAAAAAGCCTTTAACATCAAATGATATTAAACTATATCCTATTGGACACTGAGGAACAGTTCCTGGACAAAACTTTATTTATACACATTTAAATAGAGTAATTAAGAAATATGACTTAAATATGTTTTATATTGAAGGTCCCGGACATGGTGGACAAGTTATGATTTCAAACTCTTATCTTGATGGATCTTATTCAGAAATATATCCTGAAATATCTCAAGATGAAGCCGGACTTGCAAAAATGTTCAAAAGATTCTCATTCCCTGGTGGAACTGCAAGCCATGCAGCGCCTGAAACACCTGGTTCAATTCATGAAGGTGGTGAATTAGGATATTCTATAAGCCATGGAACAGGAGCAATATTAGATAACCCTGATGTTATTTGTGCAGCTGTTGTTGGCGATGGTGAAGCAGAAACAGGCCCACTAGCAACTAGCTGATTCTCAAATGCTTTTATTAACCCTGTAAATGATGGAGCAATACTTCCTATATTACATTTGAATGGTGGAAAAATTTCAAACCCTACACTTCTTTCAAGAAAACCAAAAGAAGAAATTAAAAAATACTTTGAAGGTTTAGGCTGAAATCCAATTTTCGTTGAATGAAGCGAAGATAAAAGTAATCTCGACATGCATGAATTAATGGCAAAATCATTAGATAAGGCTATTGAATCAATTAAAGAAATTCAAGCAGAAGCAAGAAAAAAACCAGCCGAAGAAGCAACAAGACCAACTTGACCTATGATTGTTTTAAGAACTCCTAAGGGATGAACTGGACCAAAGCAATGAAACAATGAAGCTATTGAAGGAAGCTTTAGAGCTCACCAAGTTCCTATTCCTGTTTCAGCATTCAAGATGGAAAAAATTGCTGACTTAGAAAAATGATTAAAATCATATAAACCAGAAGAATTATTTGATGAAAATGGAACTATAATAAAAGAAATCAGAGATCTTGCACCAGAAGGTCTAAAAAGAATGGCTGTTAATCCAATTACAAATGGTGGAATCGATAGCAAACCTTTAAAATTACAAGATTGAAAAAAATATGCTTTAAAAATTGACTATCCTGGAGAAATAAAAGCCCAAGATATGGCAGAGATGGCAAAATTTGCTGCTGATATAATGAAAGATAACCCTTCATCATTTAGAGTATTTGGACCTGATGAAACTAAATCAAATCGTATGTTTGCATTATTCAATGTAACAAATAGACAATGACTAGAACCAGTTTCTAAGAAATACGATGAATGAATTTCACCTGCAGGTAGAATTATTGACTCGCAATTAAGTGAACACCAATGTGAAGGTTTCCTAGAAGGTTACGTATTAACCGGAAGACATGGTTTCTTTGCATCTTATGAAGCATTTTTGAGAGTTGTTGATTCAATGCTTACTCAACATATGAAATGAATTAAGAAAGCTTCAGAATTATCATGAAGAAAAACCTACCCTTCATTAAATATTATTGCAACTTCAAACGCTTTCCAACAAGACCACAACGGTTACACTCACCAAGACCCAGGGCTTTTAGGACACTTAGCTGATAAAAGACCAGAAATTATTAGAGAATACTTACCGGCTGATACAAACAGTTTACTAGCAGTAATGAATAAAGCCTTAACCGAAAGAAATGTAATTAACTTAATTGTTGCTTCAAAACAACCAAGAGAACAATTCTTTACAGTTGAAGATGCTGAAGAATTACTAGAAAAAGGTTATAAGGTTGTTCCTTGAGCTTCAAATATTTCAGAAAATGAAGAACCAGATATCGTATTTGTTTCAAGTGGTGTTGAACCAAACATTGAATCATTGGCTGCTATTTCACTAATTAACCAAGAATATCCACATCTAAAAATAAGATATGTATATGTTCTTGACTTGTTAAAATTGAGATCTAGAAAAATAGATCCAAGAGGAATTAGCGATGAAGAATTTGATAAGGTATTTACTAAAAATAAACCTATCATATTTGCTTTCCATGGATTTGAAGGGTTATTAAGAGACATCTTCTTTACAAGATCTAACCACAATTTAATTGCACACGGATATCGTGAAAACGGTGATATTACAACATCATTCGATATTAGACAATTAAGTGAAATGGATAGATATCATATTGCTAAAGATGCAGCAGAAGCAGTATATGGAAAAGATGCAAAAGCATTTATGAATAAATTAGACCAAAAACTAGAATATCATAGAAACTACATTGATGAATATGGATATGATATGCCAGAAGTTGTTGAATGAAAATGAAAGAACATAAACAAAGAAAATTAATTTTAGTTTAAATAATTTCTTATATTTATGGTAAAATTGACTAAGTTAATTAACGAAAAATAACATAGGAGTACACATGCAAAAAGATATTCACCCAAAATACAACCAAATTAAAGCTGTTTGCTCATCATGCAATTCAACAATAGAATTTGGTTCAACTGCTGATAAAGTTACTTTAGATGTTTGCTCTAATTGTCATGCTTTCTACACAGGAAACCGTACAGATGTTAAAGCTCGTGGACGTGTTGAAAGATTTAATAAGATATTAGAAAAATCACATAAAAAGAACTAATTAATTAGCATTAATAAATAGGCGAAATGCCTATTTTTTATTATGTGCAATAATGCATACCAACTGCATAACCAATGTTTTCATTGCTATTGTCTCATTTCAATGAAATATTCCCGGCTTTTACTTCTCCTTTATTTGCATTTAAAGTCTTAGCCAAGCCTATTGAAGTTCCAATAATTCCGACAGTGCTTGTTACTACAGACAAAATAGTTGAAATTAAAGCAGTAATTGCAAAACCTCCTACAATATTTTTTGTTTCTTCTTCGCTCATTAGTTTGAAATTTATATTCATTTTTATTTCTCCCTTCAATTTCAAATATAACAAATATTCCATTATGTTGAATGTATTGAAGAAAAAATAGCGAAAAGTAACAATCTTTTTCGCTATTAGTAATTTAAAATTGTTATGCTCTTGCCATTTGCTAATATTGCTAATAAATCTAAAAATATTGCATATTTAATCTCATTGGGTCTTCAACAAATGTTATATTCATTCTTTATCACTATTTTCTTTAGTAATACTTATTTCTTATTTGATAAGCAAAAATTTAAAGTCAATACTATTTTTTATACACTAATATTTAGAATTGTTAATTCTTCAATAATTCTTATTGTATTGACTTTGATAAATAACTTTGTAAACTATTTTGTAACCTTTGCAGTATATTCATTACTAATATTAATATGTAGCATAACAATAATCTTTGCGAACGAAAAATTAATTTAAAAATTAAGCAAGTTAAAGAACAAAAAAATACAGTTTAAAATTCTATGAATAAAAAATTAGACTCATATAACTTAAGGGTTGAGAGTCTAATTTTTAAATTTGTATTTTTATTATTATTTTGTTGAATCAAAAGTATTTTCACTATCAACGTTTTTATCATTGGCTAGTTGTTCTTCTTTACTTTTATTTTGTTTATTATGAAAGCATAAACCTATTATTATTGCAGAAATTATTGTTCCAATTCCTCCACAAATTAAAAATGAATCTGCGAAATTGAATGTTCCTTTATCAATTCATGGAAAATATATAATATCTCTTACACCTTGAAACTGGAATCTATCATACATATTTCCAAGCGAACCTGCTGCTGTAACTGCTAGTCCTGCAACTACAAAAATATATCAATGATTTTTAAAAAATAAACTGCCAATGAAAGTTGCAAAAATTATAATAAAACTTATTATGTGTAATCCAACGTTGCCCAATCCTAATTCCAAAGTAGTTCCAGCATGGAATACAGTTCTATATCATAAAAATGCATGGGCTCCGGGTTGACCAAATGAATAAATAGCTTCAGAATCTCTATAATCTGCCGGAATATAATAATTTCTTTTAGTTAATAAATCAATTAAAGCAAATATTATAAATATTCCTATATAAACAGAAGCATTTATTAAAGCATTTTTTCATTGGCTCTTCCAATATTCTTTAGTAAAAATGGAAATTTTTTTATTTTGCATCAATTACATCCTTGCATCTTAAACAAATTTGCAATTCATCATCATAATCTGAATCTTCAAAATGGTTTCAACATCTTAAACATTTCTTAGTTTCTAATTCTCTTACTTCAATAGTATTGCCAAATGTTACTTTTGCCACCATTAATAATCTATTTAATGGCAATGATTTTATAAAATCTGAATCGCTTTTTATTACTACTCATGCTTCATTTGGTCTCTTTATTGCTTGCGATTTAATTTTTTCTTCAATTAATTTGTATACTTCATCTTTTATTTCAAAGAATTCTTTTCATTGATCTTGTAATTTAAAATCAAAATCTTTTCTTTCTAAAAATGACAATAAATGAACGGATGGCAACTTGTTAGGTTTATTGTAATATTTATAAATTTCTTCGGTTGTAGTTGGCAATATTGGGGCAAGAGCTTTTAATAAGACCTCAATAATGTTGCTAAAAACATATTGAACCATTCTACGTTCAGGGTCATCTTGTTTTTCAAGATATAAAATATCTTTGGTTATAGAAATATAGTAACTACTTAAATCAATAACAAAATTATTAATATCCTTAATAACATTAACAAATCTATATTGTTCGTAATTCAATAATATATTTGCTTCTAGATTATGCAATTTTTCTAACATTAAAGCATGAATTGAAGTTGGCGTAATTTCTTTAAAATCAAAGTCACTAATTCCACCAAGCATGAACTTAATTGTATTTCTAACTTTACGATAAATTTCAGTATTTTGGTTTAATATCTTGTCGTCAATTGTAACATCAGCAGTATATTCAGAATTTGCTGCTCAAAGCCTTAAAATATCTGCTCCATATTTATCAATTACAGCTAAAGGATCAACAACATTGCCTTTTGATTTTGACATTTTTTGGCCTTTGCCATCTAAAACAAACCCATGAGAAACCAAAGCTTTAAATGGGCTAGTATTTCTTCAAGCTACTGAGTTAATTAATGAACTATTGAATCAACCACGATATTGATCTGAACCTTCTAAATAAAGATCAAATGGAGCTTTTATTCCGCCTGGTTCAACACTTATTGAAGTTGAGCCCGAGTCAAATCAAACATCCATAATGTCTGTTTCTTTTTTAAGACCTAAATTTCTAAATTTTTCTGGCAATAGTTCATCGGCTGTTCATTCGTATCAAACATCGCTGCCATGTTCTTTAACTAGATTAATTACATGTTCAAAAATTTCATCATCTAGAACAGGATTTTTGTTTTTATCATAAAAAATAATTATTGGAACTCCCCAAGATCTTTGACGAGAAATGCATCAACTTTCACGATTTTCCAACATTAGACTTAAACGTTTTTTGCTTCATTCATTGAATGTTTTAACTTCACTTAATGCCTTAATGATTTGTTGTTTTATTGGTTTTAATGAAACAAATCATTGTGGTGTTGCTCTATACATAATTGGTAAGTGAGTTCTTCAATCATGAGGGTATGAGTGTTTAATTTTCTTAAACGCAATTAATTGATTATTTTCTGCTAAAAATTTGCCAATTAATGGATTTGCATCATCATAAAAAACACCGCTATATTCAAAGCCTTTATCATTGATTGTTCCATCATCGTTTATGTGCATAATCATTTCTAGGTTTTCTTTTTTGCCGATAATAAAGTCATCTTCACCAAATAATGGAGCAATGTGAACTAAGCCAGTACCAGTATCTAAACTAACATGATGACCAAAAACAACTGGCCCCACTAATGGGGTTATAGGATTTTTATATTTAATTCCTAAAAATTCTTTACCTTTGAATGTTGATAGAACTTGATATTCTTCTCATTTAGCAACTGCAGCAACTGATTCAACTAAATCTTTTGCAATTATATATTTGTTTCCATTTACTAACACTTTTGCATATTCAAATTGATCGTTAATTGCAACGCCAGAGTTAGCCAACAATGTTCATGGGGTTGTTGTTCAGATAATTAAATAATCTCCATTTTCAACGTATTTGTTTCCTTCGATCACTTTGAATGCAACATATAGAGAAGGTGAAATGTGATCAGCATATTCAACTTCTGCCTCAGCAAGTGCAGATTGAGAAGAAGGTGATCAATAAACTGGTTTCAAATCGCGATAAATTAGGCCTTCTTTAACCATTTTAGCAAAAAGTTCTAATTGCTTAAATTCTAAGTTTTTATCCAATGTCACATAAATTTCTGAAAAGTCTGTCAATAAACTTAATTTCTTAAATTGAGCCTTTTGATGTTCAACTTGACTTAAAGCATATTCTGCTGCCTTCTTTCTTAATTCAATAACACTAAAATCTTTAGCATTCTTTTTAGCTTCTAGCAACATTTTATGTTCAATAGGCAAACCATGTGTATCTCAACCCGGAACATATGGCGAATAATATCCTTGCATACTTCTAAATCTTACAATTATATCTTTTAAAATTTTGTTTAGAGCGTGTCCAACATGAATATTACCATTTGCATATGGTGGACCATCATGCAATACAAATGATTGACTGTTTTTATTTCTATTTAAAATAGTTTGATACAACTTAATATCTAGCCATTGTTTTTGATATTCCAATTCTTTTTCAACTAAATTTGCTTTCATTGGAAAATCAGTATTTGGCATCAATAACGTGTCTTTATAATCTTTCTTTTCTTCCATTATTAACAAACCTTTCTTTTTCTATTTAATTATGAAATTTAAAATTTTAAAATCTTTATATATTACTTTAACTATTTCATGACCTTGTAATCATTTAGCAACATTGTCTAGTTTCTTTGCATTTTCAATAATTTCTTCATTGCTTCAATTTGGCAATATTTCAATTTGAGAACGAACTTTTCCGTTAATTTGAATACCAATTTGTATATTTTTAATAATTATCTTGGTTTCATCAGCATAAGGTCAAGTTTGATATTGAATTGGTTTTTCATTCAACATAAACAAAAGTTCTTCAGCTAAAAATGGCGCAAACGGACTTAATAAAATAGCAAATTGTTTTAAAACAAATGATGATGGAATGGTTTTTAAAGATGTTAAGTAATTAGTAAACACCATCATTTTGCTTATTGCAATATTAAACTTAGTTTTTTCAATATTTTCGCTAACTTCTAAAATCAATTTATTAATGTTGCTTATTAATTCATTATTACTATCGTTTATATCAATATTTCCTTTGACAAAATATTCAAATATATTGTAGATTTTTTCAATTCATCTTCTTATACCATTTAATGAATCTGTACTTCAAGATTTTGATTCCACTAAAGGCCCCATAAACATTTCATAAACTCTTAAACTATCTGCTCCATGAGATAAAATAAAATCATCAGGATTTATAACATTACCTAGAGATTTAGACATTTTTTGACCATCAGGTCCCATAATTAATCCTTGGTTTATTAGCTTTGTAAATGGTTCTTTTGTATTAACAATACCCAAATCAAATAAAAATCTATGTCAAAACCTTGCATACAATAAATGCAAAACAGCATGTTCTTGCCCACCAATATACAAATCAACCGGCATTCATTCTTCAAAACGTTTTTTTGCTTCCAATGAATTTAGTGGAGTATATGAGCCATCTGGGTTTTTTAATATATAAGCCAAATAATATCAACTTGAGCCAGCCCATTGTGGCATAACATTTGTGTCATGATGATATTTTTTACCGTTAATTTCAACATTCATTCAACTTTCAACATTTGCCAGTGGACCAGAGGTTGTACCTGATGGTTTTATGTTTTTTATCTTTGGCAATTCAACTAATTCTTTTACTAGATAAATATTATTTTCTTCATCAAATAAAACAGGAAATGGTTCTCCCCAGTATCTTTGCCTTGAAAAAATTCAATCGCGTAATTTATAACTTACTTTTTCATCTGCCAAATTATTTGCTTTCAATTCATTAAAAATACATTTTACTGCGCCTGTGTTGTCTAAACCATTTGCAATATCTGAATTGATATGAATACCATCTCCTAAAAATGGTAATTCATCACATTCAATAATTTTTTTAATTTTTAAATCAAATTTTTTAGCAAATTCATAATCTCTTTGATCATGGCCAGGCACACACATTACTGCCCCACTTCCATATGACATCAAAACGTAATCAGAAACTCAAATAGGAATTAATTCATTAGTCAATGGATGAATTGCATACGAACCAGTAAAATATCCTGTTTTATCTTTATTTAAAGATGTTCTTTCTAATTCATTTTTTAATTTAACTTCTTTTAAATAGTTAGAAATATCATCATGATATTTTGCGGTAGTTATTTTTTGAAGAAGTGGGTGTTCGGGAGAAATTACTATTGCAGTTACACCATAGATAGTATCCAATCTAGTAGTAAATGTTGAGAGTTGCAATTTTTGTTGTTCACTTGAACTAATATTTCATTTGACAATATGTCCTTTACTTCTTCCAATTCAATTTCTTTGCAATTGTTTTAAACTTTCAGGTCAATCTAAATCTTCTAGGCCTTCAAGTAATTTATCTGCATAGGCTGTTATTTTTAACACTCATTGTTTCATAGGCTTTTTAACGACAGGAAAATTACCCCGTTCGCTAACTTTAATACCGTTTTTTTCTAAAATTTCTTCATTGGCTAAAACAGTACCTAGCCCTTCGCATCAATTAACATCAATTTCTTCAATTGAAGCTAGTCCTTGTTTATACATTTCTTTGAATATTCATTGAGTTCAAATATAAAATTTGGGATCAGTAGTATTTACTTCCTTGTCATAATCATAAGAAAATCCCATTGCTTGCAATTGTCTTCTAAAATTAGCAATATTTTTTAATGTAAAAGGTTCAGGATCATTTCCTGTTTTTATTGCATATTGTTCAGCGGGTAAGCCAAAAGCATCTCAACCAATAGGATGCAACACATCAAAACCATTTAGACGTTTATACCTAGCAATAATATCAGTAGCGGTATAACCTTCTAAATGCCCAACGTGCAATCCCATTCCCGAAGGGTAAGGAAACATGTCAAGTATATAGGCTTTTTTGTTGCTATTATTTCCTGTGGCAAACGATTTATTTTCGGCTCAAAATTTTCTTCATTTTTGTTCTATTTCATTGTGCTTATACATAATATTTAAATTTTACATTAATATTAATTAAAAAAATAAGAAATAAAAAAAGTTGCGAAAATTTAATTGATCCAAAAAAAATAATTAATTTAAAAATAAGCCAATGCATTGAAATTTCTAATTAATATGTTTAAAATTTTTCAATATTTAGAAAATATTACAAATTTTTATATCTATCCTGTAAAATTTACTTATTTAAAGACTAAAAACAAAATAAAGTTAAAAAAGTTTTAGTTTTATTAATAATAGAAATAAATTTAATCAAAGAGGTATAAACATGAAAAGAAAAATTTTACTATTTACCCCCTTAATTATTGCTGGTAGCTTAATACCAACAACATTGACAGCATGCAATAATGAAGATAGCAAACTTACAAAGCAATTAAAAGCAAAGCAATTAAAAGCAAAGCAAACATTTAATAATTTAAAAAATAAATTGCAAGATTTATTGAACTCAGAAGATGCAAAAGATATTGATGCAAATAAAGAAATTAATACACTTGCAAACACAACAATTGATGATAATTCTCAAATACAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTGACTAAGAAAATTAATGATAAAAAAATCCTAATAAAACTAAGACAAGATTTCAACAAAGCAAAAGAAGATCTAAGAAATCTTTTGGCGTCAAAAGAAGTGGCGGATATTGAAAAAGAAATCGAATTAAACACTCTAAATACCACTGATATTGCCAAAGATTCTTTACAACAAGAAATTCAAACCAAAATACAAAAAATTCAAGAAGCTATACAATCAATAAACAAAAAAATCAATGACAAAAAGCTATTAAACAAAGAACAACAAAATCTTGTTGAAGCAAAAAAGACACTTGAAAATTTAATTGCTACAGAAGATGCAAAAACAGTCGGAATTGATGAAGCAAAAAAAATTCTTGACAATAATAAAAATAATAAAATATCAACTATTGAAGAAATTAAAAAAGCAATAAACGATTTGAATGATATTACAGCCAAATTAAATCAAAAAATTGCAAACAAAAAGCAAGAATTATTAGAAAATTTCAAAAAATCTAAACAAGATTTAGAAGAATACATAAATAAAAATTTAAAAGAAATCAGATTTATTGAAGTTAAAGAAAAAGCCCAAAAAACCATTGAAAAATTTTCTAATATAAATAAAGATAATTCCTCAATCAAAGAAATTACAGATGCTACTAAATCGCTAAATAAAGCCAAAGAAGATGCTTTAAAAGAAGAAGAAGGAATCAAAAAATTAAACGAAGTCTTGGAATATGTCAATAAAAGAAATGAACTAGAAAAATTAATAAAATCATCTAATACTCAATCAAATGAAGATGCTATTAAAAAAGCCCAAAAAACTTTAGATGATAATAAAATAGCAAATACCAACGAATCATCTTCAAAAGATTTTGAAGAAAAAAGAAAAAATATTGAAAAAGCAATAATTGAACTAAAGGCAGCAATAGAAGCAACAAAAAAATCTAAGAATTAAAAATTCATATATGTTGAATTATTCATTCAATGTAGTTAAACCCAAAATAATTTAAGTTGTTATAAATTATTTTGGGTCCCAGAATGAAACATCAAGTGCAACACTTTTGTGCTTGATGTTTTTATTATATTAAGTTATTAAAATTTCTATGCGATTTTTAAAATTTTTGCATAGAAAAAACGTTGTGATAAACAACATGTTATATTTTTTAAATTTTTCTAGTTTATTATTTCGCCAAAATTTTCATACATAAACAATTCATATGCAGATTTTCAGTTTAATATTTCTCTTGGCATATTATTTATTTTAGCTATTGTAGTGTCTAAATCTTCTTGGGTAAGAATATTAAAATTAAATCCTTTTTGGTATTCTCTTCTTATCATGCCATTTCAATTTTCATTTGAACCTCTTTGAAATGAAGCATAAGGTTCCGCTCTATAAATAATAATATTTAATGATTTTGCTAGAAAGCCAATTTTTTCAAATTCTATACCATTATCGATTGTTATAGTTTTAACGATAAGATTTTTGTTTTTTATTAATTCTTTGATTTTGCTATTGATTTCAAAAGGAGATTTAGATGACACAAAAGTAGCAAATCCAACTCTTGTTTTTCTTTCTGTTAAAGTTAATATATTTTTATAGCCATTTGCTCTTTTTCCAAGAACCAAATCTGCCTCTCAATGCCCGTATTCCAACCTTAAATCAATATTTTTTGGTCTTGTTCATATTGGAAACACATAATTTGCACTTTTTATTAGACGACTAAGAACCGAAGCAGTTCTTTTGCCTCCTTTTTTATAATATTGCCTTAATTTATCGGATTTTCTTATTTTTCATTTATTTGTGTTTATTCAATTGAAAACGGTTCGCATTGTTGGTTTTTTAACATTAAAGTTTTCTTCAATATAATTCATTGTTGCTTTAACGCCAAAATATTTTTTGTCATATTTAGACTTAAATATTTTGGAAAACTCCTCAAATTCAAAATTTATATTGAACTTAAACATGTATTTATGATATGCTCTAAATTTCGATTTTTTATCAGCTCTCAAAAATATATATTCGTTAAAATTTGAATTTCTTTTTATTTCTCTTGAAATAGTAGATGCACTTCTATTCAATTGTCTTGAAATTGCTCGTAATGATAAATTATTTTCCAACGATCTTTGAATAAAAAATCTCTCTTCTAATGACAAATGAAAATATTTATGTATGTTATAATTCATAAAGGCTCCCTTGGTACGTTTGTGGAGCTTTTTATTTTAAAAAAATTCAAGTGCCCCACAACTAAATTTTATTTCAAATTTGTGTTGCACTTGAATTTTCATTTTGGCACCCAAAATAATTTAAGTTGTTATAAATTATTTTGGGTTTTAATATTAAATGATTTTTGCATATTATATTTTTATCCAAAAAATATATATACTGACATTAAAAAACAATTAAATGTTAAGATTATAAATATAAATTAATTTATTTTAAAATTATGGAGTGTAAAAATCTATGGGTAAAAAAGCAAAATTATTTGTCTTTGGTGGGATTAGTTTTTTAGTGGCGGCTTCAATTATTGGTTCTGTTGCAGCATATGCTTCAATCAATAAAAAGAATGAAATTAGAGTTAGCGAATATAATAAATTTCTTGAAGAAAAAACTAATTTAAATTCTTTTAGCAATTTAGAAAATGATAAAAAAATTAGTTCAATAAAATCTAATACCTTAAGTGAAGTTGAAAAGATAGATAAAAATTCAGATTTATTTACTATTCAAAAAGCCCGCAAAATAGTCAACGAATCCATTGAAAATATCAAAAATATAATAGCATCGCATAAAAAAATGTTATTAGAAAAAGAAAAAGCGTTTATTCAATATAACAATGTAAAAAAGCAATTGCAAGATATGCTTTTAAAAATTTTTGACTTTTTGAATAAAGAAAGCCAAGGACATTATTACATTGGCGATGGAAACGAAAAAATAATAAACAAACAAAATTGACTATTAAAAGAAATAAATGAAAATCAAAAATATTTTTCAAACATAACGATTGATTCGGATATAGATAGCATAGAATTGGCAACAACTAAACTGGTTGAAATAAAATTTTTAATTAATGATAAACAAGAAGCATATAAATTTGCCAAAGAATATAACGACTTATTAAAAGAAATCAAATGATTTATTTCAGATTGATATGAATTATTAAAAGATCCTGATATAAAAAAACATGAAGGTCCTTATGGCTATAACTCGTCAGAAACTTTATTCGTATATTTTTTATTTAAATACAAAAATGATGGCAAAAAAATATTTACAAAATTTAAAAATTATGCACAAGAAATGTATGACAAAACTGCACTTCTTGAGGAATTCGACATTGATTTTTTAGGATTCAAAATCAAAGATAAAAACTTCTATAAAAATTATGTTGAGTTATTGACACCATATAAAAAGGCTATTGATTTGTGAAAAAATTATTATGATTCATATTCTATAGGGCTAAAAAAACTAAAAGAAATTATTTGAGACAAATTGTATATTAATGGTTATACTATCTTTTTTAAACCTAAAAAAATATATGCTTTGATAAATCAACCTGAACTAGACAAAGAACTAACTGAAACTACTGATGATTATAAATTTAAACTAATGCTTATCCCAAAAGAAAATACTATAGAACATAATAAATTAATAGAAAATTTTAAAAACTATTGCGACGATTTAATGAATAGAAGCAAAAAATTAAAAAACGACAATCAATAAAGGAGAGACGAAAATGAAAAAAAGAACAAAAATTGTAACTTCATTAAGTCTATGCTTAACTACTGCTATTTCAACAATAGCAATCTATTCAATAATCGCCGTTAAAAAAAATATAGAACTAGATAAGTCAAATAAATATTCATTATTTATACAAGAAAAATCTAGATTGCAATCGCTTGTTGCAAATAATCAACACAAAATTCCAAATAGTTATTTAAATTCAATTCAATATTTCCATAATGTAACTAGAACCTCAAAAGCAATAGAAATAATTGATGCAACATCAAAAATAAGAAGTGAAATTATTGACGTAAATAAAAAAATTCTTGCCTTTGATTTAGAAATAAAAAATCAATTATCGCTATTCAATGAATTTAATCAAATAAAACAAAAGCTTGAAAATGATTATCTTAACAGACTATATAAAATATGAAGAGCAGATGCTGGAGAACCAAATATGATAGTTGATTTTGGTTTTTTTGAAGAAGAAACTCACTTTCAAAAATATCTTGAAACAATTAAGAAATATTCTTGAATTTCAAAAAATTCATCATCATTTGAAATAAAAAGAGCTATCTTTGATTTGAAAAATATTGAAAATAATATTGATTATTTTTTAGTTAAAAAAGAAGTTGAACATTTACTTGAAAAAATTGAAAGCACTATTTCAATAGTTGAAACTAGACTAGAAAAAAATAAAATCAATAAAATATTTGAGGATAATGAAGTAAAAGAAATTTTAGATTTAATTTCACAATTTAAATTAGATTTACAAAAACAAAAAGATTTATATAACTCAGTAGACAGTAAAACTGATAATATCAAACACGAAACTTTTAAAAAAATATTTAGCCATTATAATAAAAACAATTTCTTTAAAATTTTTGATTGCATAGATCAAAGAGTTGAATTATATTACGACTTTATGTTCTTTTACTCGCCTTTATTCTTTTATGAACAAACAGAAGAAATCAAAAAAGTTTTCCAATATCCTAATAAAAAAGATTTTGAAAAAGAAATAATTTTAAAATATAATCAAGCCAAACAAAATCTTACTAACTTCAAATCAAAGATAAATGGAAATGAAAAAATTGCCAAAAAAGCATTCAATAAATTCAATGAATATATACGCAATTTAGCAATAAGTTTTGAAAAGGAATTAATACAAAAAAAATTATTATTATTAAAAAATTTATTAACTAAATATGATCAAAAGCAAGATGGAAAATCACAATTCATAACAGTTGAAATAAAAAAAGCCATAAATGATGCAGAATCTTTATTAAGTTATAATGACTCGCAATATTCCACAAGAGATAAAAAGAGTATTTGAGAATTCAGGGTAACAAAAAATAATTTGCAAGAATTAATAGACAAATATTAAAAATCCTTGAATTGTCCTGCCCTTGCAATAATCAAAAGATTATTTCATACACAATACTAAAAACAAGATAACTGAAAATCATTATCTTGTTTTTATTTTTAATTTGGCAATATTGAAATTAAATTTAATTGACTTTTTGTCTAATTGAATGTAATTTATTTTGTAATTTAAGTAATATTTCGGTTAAATATTTTAAATTTGGCTGTGATTTCTTAATATAAAAATCTTCTAATTTTTCTTCGTGTTCTTCTACAAGTTTTCTAACTTCTTGTATTTCGTTAGAAATTTTTTGATTTTTAGAATAGACATCATTTGCAAGAATATTAAATTCCTCAACATTTGATGATATCAATTTCAATAAATTTCACCTATTTAAAATAATTTCTAAGTTTAATTTTCTAGAAAGTTCGTATGTAATTTCTATATTTTTATTGATTTTGGCAATATCTTTTGAAGAAAATAGCTCAGAATTTTCTTTTAATATTTTGGCTATTTCATTATTTAGAAATTTACTTAAAAAATTTCCATCTTCTGAATTTTTTTGTTCTTTTAAACTTTCATTAAGCGTTTTTAAAAGTTTAATGCTTTCTTCTTTATTAGTAATGTTTAAAAAATCCTTATTTATATTTTTATAAATTTCTTCTTGGACAGTTAGAATTTTTTTAAATTCTTCATGTATAGATTTTTTTATTTGTTGATCATTTCTATTATTTTCCAATTTATTAATTGTTGCTAAAAAATTTGTAATTTTATTTTTTGTTTCTTGACTAAATAAATTAAATAAATCGCTATTTTTTTGTTGCATTTCAAATTCATTCTTTGCATTATAAATAATTTGTGAATATAATTCAATATATTCGGCCAATAATTTATTTGATTCTTCAAATTGATAAATAACTTCTGCAAATGTTTTTGAATCAATATCCTTGAAATCATTAAATAATTCGTTCAACATTAGCCTATCCACAACATCTCATGTTTTGCTATTTTTAAAATTATTAAAGTTAATCAATTCTTGTATAAGTGGATTTAAATCATTCTTAACATTATTTAAATGTTTTTGTATATCTTTTGATGAATTTATTTTTTGATAAATATCTTCAATTCTTTTTGAAATTTCACTAGTTAATTTCGCTATATCTTCGTTTTTAAAGCTAAAATTAACATTATTAATCTTTTTAAGAAGTTCTATTTCCTTGCTCATATCTGTTTTGTTTCATTTTTTATAAATACTAAATAGATTTTCTAATTTTCTTTTTTGTTTCAAAAATTCAACATCAATTTTTGATTTTGGACTTAGCGACTCTTTAGTATATCTTTGGTCAATTAAATTCTTTTGAACGCTAATGTTTCAAAGAATTTCTTTAGATTTCAAAATAAATTCATCAGCCTCATTTTTATCTTTTTCAATAAAATCTTTAATTTCTAATTTTAAATTTTCATAAATAAAATCTTTAAGATTATTGGCTATATATGTTTGCAGTTCATTTTTAACTTCTTGTAGCCTACTAGGACTAAAACTTGCTACATTATTTTTATCTTCTTTTGGTTTTTGAGATTCATTTGGAACAGTTGGCTTTATATTAGGACTAACTATGTGGCTATTATCTTCTTGTTTTTTATTAGATGTATTTGGTTTAACATTAGGCGTATTTGCTACGTTGTTGTTTGAATCTTCTTTTCTATCTTTAGTTTCTTCTTTTTTATCTTGTTTATTAGAACATGAAGCACTAGAAATTACTGTAAATACAGTTGCAAACGATCCTAAGATAGTTGATAGAATAAATAGTTTGTTTTTTCGTTTCATATGTGTTTCCTTTGATTTGATATTAATAAATTATTTTTAAATAATTATTAATATAATATAGAAATATCAAAAACTTATATTTAATTTAATTGATTTTTAAAAAATTAATAAATTATTCTATTAAAAGTACAAAATTAGATTTGCTAACAAAAAATATATTTTTTTAACTTTTGTTTCTAATATATTATATAATTATTAAGCAATTTAAAGGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCATGTTGCCCTTTAGCCAAGTGGAAAGGCAGCGGTTTTTGGTACCGCCATCCGTTAGTTCGAATCTAACAAGGGCAGCCATTTCATTAAATTGTCCAAAAAAGCGCAATGCGCTTTTTTATTTTATTAAAAACCTTCCGATTTTTAAGTTTTACTATCGGAAGGTTTTTAACAAATTATTTATATTATTTGTTCATATTATATTTATTTTTATATTCAAGCAATTTGGCTTGTATTTTAATATCTTTATTTTTTAGTTCTAAATTGCTATGTTCTCCTGGGTTGTCCAAAATTTTTTCTGCTTCTTGTATTAATTTGTTTAAAGCTTCGTAGCCTTCTTTATATTCAACTTTTCCTTTAAGATTTTTTTCAATAAAAGAAGATGTATTACTATTTGTGCCATACAAATTATTTATGAAATCAAAAAATTCACCAATAATTTCATTCATCTTATCTTCAATTGGTTTTTTTAATGCTCTAAAATCAGATTTAAATTTTTCTAATTTATCTTCCTGTATTTCTTGTCCTTGCAACTCAAGAGCATCCATTCTAATCCTTTCAATTTCTTTTTTTGGTTCTTCTCAATATTTTTTGCTATCCAAACAAGTTAAAGATTTGCTTTGCATATCTCCTATAAATTCAGGTACTTCTAGATTTACGAAGCAAGATATATCGCAACTAAATTTATCTTCCTTTGCATTGTTTAGGTCTTTGGTTAATCATTGCTTAGCCACGGATATTTGTTGAATGTCAGAAGCAGAAGTAATATCTTTAAAGAGATTAAGTTGTGCGTTTAAATGGTCTATTATTTTTTTATAATCATAATAGTTACTATTATATTCTTTATAATATTCATCCTCTTTTACTTTTTCAATAAATTGTTTAATTTGTTCCCTGATTTTTTCAAGCCCTTCATATTCAATATTTTCCTTTGGTTTTTTATCATTAATTTTCTTAGTTAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGACTCAATGTCTTTAATTAAATCTTTGTTAGTAAGATTTGTGTTGTCAAAGATGTTGGTTTCTGATTGTTTATCAACATCTTTGGCTTCATTTGATTTAATCAAATTATCTAGTTGTTGTTTCTTTGAATCAAAGTTTTGTTTAGCATTTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAACAATTTGATCGGTCGTCGATGAAATATCTGCATTGTTATTTTTCAATGATTGATTTGCATTAGATTTGTCGACTTTTTGTGCGTCTTGTGAATTGATTAAGTCTTCTAATTGCTTTTTAGCGTCATTAAAGTCTTTAAGAAGATTGTCTTTTTGGTTCTTTTTATCATTAACTTTCTTAGTTAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGACTCAATGTCTTTAATTAAATCTTTGTTAGTAAGATTTGTGTTGTCAAAGATGTTGGTTTCTGATTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATCATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTGTGGCTTGTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCATTTGTTGCGTTAATAATTTGATCAGTCGTTGATGAAGCATCTACATTCTTGTTTTGCAATGATTGATTTGCTTTAGAAGTGTCAACTTTTTGACCATCTTGTGAATTAATTAAGTCTTCTAATTGCTTTTTAGCGTCATTAAAATCTTTAAGAAGATTGTCTTTTTGGTTCTTTTTATCATTAATTTTCTTGGTCAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGACTCAATGTCTTTAATTAAATCTTGGCCAGTAAGATTTGTATTGTTAAAGGTGTTAGTTTCTTCTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATTATCTAATTGCTGCTTCTTTGAATTAAATTCTTGTTTAGCTTTTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAACAATTTGATCGGTTGTTGATGAAGTATCTACATTATTGTTTTGCAATGATTGATTTGCTTTAGAAGTGTCGACTTTTTGGCCGTCTTGTGAATTAATTAAATCTTGCAATTTCTTTTTAGCGTCGTTAAAATCTTTAAGAAGATTATCTTTTTTATCATTAATTTTCTTAGTTAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCTTTACCAGCAAGATTTGTATTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATTATCTAATTTAGATTTTGATCTATTAAATTTTTCCTTTAATTTGTCAATATTATCTTCTTGTTCATATTGTCTTCTTTTAAATTTACTTTTTAGTGATGTTGCAACTAAGGTTGCCGTTGTTGCTAGAGCAATAATTAACGAAGTAGAAAGTAAAATTCTTAATTTCGATTTTTTCATATATCCCCTTTTTTTATTTTTCCCTTATTTTTTATATAAAAACTTATAAACAATTATTAAATTCTTATATTGTGATAATTTTCAAACAAGACTTCGAAAAAAAAAAAAAAAAAAAACGGTTTTCGCTCTATTACAATTTTTTTATGCACCTTTTAGTTTATTTTAATTGCTTTCTGTTAAAACATGCTTAAAATAAACTAAAAAATCAAACTCATATTTTTAGTTGAGTTTGATTTTTATTTGTATTTGGTTATATTATTAATCTTGTGGAATATCTTTGTTTGAACCTATATAAGCAAAAATTTCAGGATTTTGTTTTAAATATCTTCCTTCCAATATTCTTATAACGCTTTCAACTGTTTCGTTTAATGGCACAAATTGACCAGGCGATTTAGTAAAATGTTCAGTCATAAAGAAATTTTGAGTAAAAAACATTTCAAGTTGAAGAGCTTTTTTAACAGTGATTTTGCTTTCTTCATCAAGTTCATCAAATCCCAATATCAGAATAACATCTTCAAGATCTTTATATGCCTTTAATATTCTTTTTGCTTCAATTATTGCGTCATAGTGGCGTTTGCCAATAATGGTTGGATTAATTGCGTTTGTAGATGAAGCAAGTGGATCAAAGGCAGGAAAAATATTTTTAGCCGCTTGAGATCTAGATAAGACCAAATTACCATCTAAGTGATTAAATACGGCTACTGCAGAAGGATCGCTTAAATCATCCATTGGTAGAAATATTGTTTGGAATGAAGTTATTGAGCCCTTCTTGTTTCTAAACAATCTGCTTTCAACTTCAGTTACATCACTTTCTAAAGTTGATTGATAACCTCCAATTGAAGGCTTTTTGCCTAATGCTGAACTTACTTCATTTTCTGCTTGAATAAAACGATAAATATTGTCAATAAATAATAAAACATTTTCATTGCATGTGTCTCTTAAATATTCTGCAGCAGTAATTCCAATTGGAACAATTGACGTTCTAGCTCCTGGTGCTTCATTCATTTTTGAAATAAACATTATTGAGTCCTTCATTAAATTAGATGAAGAAAGTTCATTGTATAATTCTATTCCTTCTCTTGAACGTTCACCCGAACCAATAAAGATATTAGAATTTGCTTCATGTTGTTTATTAAGGTTAAAAATAATTTCCTTCATTAGAACAGTTTTTCCAACTCCGGCTCCTCCAAATAAACCAATTTTTGCGCCCTTAACAATTGGCATAAAGAAATCAATTGCTTTTATTCCGGTTTCTATAACTTCTTTGTTATTTTCTAGATATCTTTCATTGTTTATAGTTGAATTCATTTCTACATATTCAATTTTATTCTTTGTTTCTAGTAAAGGTTGACCCCAAAAGTTGTAAATATTGTTTTTTGAATTTTGCCCTATTGGAACCAAAAAACTCTTATGAGTGTTAACAACTTCTTCGTTAATTGAAATATCTTTACTTGCATATACAACAATTGCTCTAACGTGTTTTTCGTCAATTAATCTTTTTACTAGTAAAAAGGTTTTTCCTTCATGCAATGTCAACAAGTGATTGATAGCAGGTAAATCTTTATTAAATTCTACTTCAACTATATCTGATCAAATTTTTATAATTTTTCCATTCATTATTTATCACCTAAAACCTTCGCTGCCTTATCTAATTGTTCTTCAGGTATTGGCACAAAATTATGTTCCAATTCAATATCTCAATTTAAATTTAAATAGTCCGAATATGATTTCAAGACAAATGCAAAATAATTCTTCATAATATTATCATCGTAGTCTAAATTATTTAAAATTGTTTCAAATGCTTTTATGCAATCGTTATAATTATTTATTAAATAATCTAGTAACATAAATGCTTTTTGTTCATCTTTTATTCCTTGCAAAATACCTCAAGAAATTAATTTTGTTGTTAGCACTACAAAACGATATGAGTAAAGTGAAAAACCCTTTTGCAAGAACATTTTGTCAATTAATTTTCCTTTGTGAAGCAAAATAGAAGTTTCTTTATTAAGCTCGTAATCTAACATTGCCAATTTTAAATGTCTCTTGTAACTTCTAAAAATTTTATTAATTTCACCCGCTATTTTGGTCATCATTCTTGATTGAACACTTGAACCTGTTCTAGAAACAGACAAATCAATGTTTATAGCAGGTAATTTTCCAGAAGCAAATAAGTCAGAAGAAGTTACAATTTGACCATCAGTTATTGAAATTATATTAGATGAAATTAAACTAGTTATATCACCATCAACTGTTTGCAAAATTGGAAGTGCAGTAATTGTTTTTCTATTAACAAAAGATCCAGCCCTTTCAAGCAATGATGAGTGGGCAAAAAAGATGTCACTAGGCATTGCTTCTTTTCCAACTGACTTGTTGGTTAATAGGGCAATTTCTCTAATAATATTAGCATGTTTAGTTAAATCATCAAATACTATTAAAACATCGTTGTCATATGAAAGATTTTCTGCATGAGCCATTCCAATGTAAGGCGCTAGATATTGTTCATAAACGTTTTGAGGCGCATCAATAATCATAGTGTTTTTCAATGCATCGTATTTTTTTAATGTTTCATAAATATTAGTTAATGATTCACGCTTTTGGCCAATTGCAACATAAATACATTTTGTGCCATTTTTTGCTTGATTTATAATTGCGTTTAATGCCATATGAGTTTTGCCTGTTTGTCTATCACCAATAATTAATTCACGTTGACCTTTTCCAATTGGAATTAATAAATCTATTGCAATAATTCCAGTATAAAGTTGTTCATTTAATGTTTTAACTGTCATCAAATTATGAGCTAGACCGAATATTGGATGATGATTGCTTAACATTGAATTATTTGTTCTCTTTTTTTCATTTGTTGGTCAAATAATGCCACCGTTAATATCAATAATTTTGCCCAAATAATCAAGACTAGTATTTATATTTTGGGATTCATTTAATTCAATTAATTCACTATTTATATCTAAAGATACGTGTTCGCTATTAATCAACATGAATGCCTTATTAGTTGTAGCAGAAATAACTATGAATTTAACATTTGGGTTACTTGCTAAATAAAATTGTTGATTTTGGCGGTAGTTATATTCTCCCTCAATTGTTGCTATGCAATTGAATATTGATTTAATACGAGGATTTTTAGTTGTGTTGTTCATGTGTTCTCCTTCTAACTATATAAATAATAGCCCTCCAATTATTAATATCAGTAATGCGATCGCTAGTATTACACTATTGATAATAATTACCTTTTTCTTTAATTTTAAGTTTTTTAACAATGCAAAAATTATTATGTTTGTAATTATTATGATTAAAGAAATAACTATAAAAGATGTCCCTAGTTTTTTAATAATCTTCTTTTGTTCAAGATAATTTTTATTTAAAACATTGACTGCTTCTAAATAAGCTTTATTGAAATTTTCTTTTTCTTCTTTATTAGCTTTTATTGAAATATTACTTGCTAAATTTGATAATAAGTCAAAATTATGTTTTATCTTAGACAATAGTTCAAGGTAATCAGAATATCATTTTTCTTGGTTGAAATCTCTTTGGCTAATTAAAGCGGTAAATTTGTAGTTTAATTGTTTATTTAATAAGAATAATTCTCTTAAAACATTCTCATTGCCTTTGATTTCTGCTAAATTTGTTTTAATGAATTTTTCATTGAATTTAAGTGCTTCACTAAATTGCAATTGTACAAGATTATATGCCTTTGCTATTGTAGTTCAAAAGTATATATTATTTACTACTAGACTATTTAAATAAGAAAAAAATGTATAACGAATTGTTTTGCCAAACTCTTCAAGTAATTTTTTTGTATTTGTTTTATCTTCTTGCAATTTAACTCAATATTTTTGCAATATTTTATTGCTAATTGCACTATATGAATCTTTGTCAGCTTTAAATGACATTTGTAGCGCTGAGTCAATCATTGCAAGCAATGCTTTTCTTAAATATCCATTTCTTAGCTTATTGTAATCTAACTTTTCATCTAAGCCCAAACCTTTGTATAATGGCTTAAATATTTCTGAAATTGATTTTATTTCAGTTTCTTTTAAGAGATTAAAATATTTGTCTTGTGACGGAGTATATTTTTCAATTAAATATGTTCTTTCTAACTTAGGATTTATATTATCTGAAATATGTACTTTTAAATTAATAATATCATTGCCACTAATATCAAATGAAAAAACAGAATATTTATAACGAGTATTTATTGGGTTATTGAAATAAAATAATTCTTGACGTTTGCTTGCATCTAATGAATCAAATTGACTTTTAATTTGACTTAATGATGAAGGCAAATTATTACTTGATATAAACGGAACCAAAAGGGGCAATGATTGAATTAAATTTTTTTCTTGTTCTTTTGTTAAGTGACTTGTTTTATCACCATCAACTAATGGTTTTAAATCAGGCAAGGAATCAGGATTTTTTTCTTCTTTTTGTTCAGCTTCGTTTGTTTCTTGATTTTGTTTTAAATCAAACTTTGTAATTCTTACTTTAAACTTTGAAATAAAATATTCATCTCAACTTTTAAAGCCTTTGGGCAAAGTGGTAGAAAATACGCCGTTTTTATCCCTAATTAATTCGACATCTTTGTCAATATTTAAAACGTCTTTTTCATTAAAAAATAACTTATTGACTTCTTTTAAAAAATCAGATTTATATTTCTTTAAAAATTGATAATACTTATCTTTTTTAACAGTATTTAATTGATCTTTAGCAACTTCTTTAACACTTCCTTTTGGCAAAATTGCTTTTTTATAATCTAGTGGATCGGTTTTACCAGTTTTTATTGATTTAAATAAATCGCCTTCATAATCAATATCTTGCAAATCGTGGTTTTTTTCTTTGCCTAATATAAGGGGAGTATTTAAATAAATTCCATACTTATTAGGATCTTTTTTAATTTCTTCTTTATTTTTTGTTAGAAATGTTTCTAAATTTTTTAAATAAATTAATTGTTGAATTTTTAATTTAAATTCTAATTCTTTATTTTCTAGCAATCTTTGTTGTTGAACTTGCAAATAATTGATTATTGCTTTTAAACCATCATCCAATGTTGAAGCAATTGTTTTTTTAGCTTCGCTTTCAAATTCGCCAAAATTTGGATCTAACTCAGGTTTTTTATTATTTGGATCATTATTTGACGCTGCCGAAACTACTATTGGAGATAATAAAGGAACTATTGCAAATGATAATAATAAGATTTTCTTTTTATTCATTGTTAATCCTATCTTTTTCAATTAAATTTAATTCTTGTCTCAAAGATAACAAGTAATATTTTTCTTCAAGAACTTCTAATTTTAAAAATTCTTCTAATTTTAAGCCTAATTGTTGTTTAGCCTTTCAAAATTTAATTTCATCTGAAAGTTCAGCTATTGCTTTAACTAAATTGACTTTATTGAGAGATTTTATATAAAAATGAGGTTGGGTTAATGTTGAAATTGTAGCTATATTATTATTCACAACAATATTTGAGTTTTTTAAAAAAACAAAGAATGTTTCGTTTTGTTTTGAAATATCAATAATTTTTAGTAAAAGGTTTTCATAAGAAGCAATTGATGTATTTGCTATTTGTTCTCATGTATTTTCTTCATTAACGTTAATATAAACCAAGCCTTTTTCGATGTTTAACTTCTTATTTTCTAAAAAAAGTAATTCAATTTTATATACGTTATTCATAAATATCACCTTCATCAGGGTCTTTTTTAGTCAATAAATTTAGTTCCTCAATTTGCAATTCACTTTTTGCTCTTGATATTTGCTTTTTAAGTTTAAGAATGCTTTCGTCTAAATCTTTTAAAATTGTATTAGTTGAAACTAAACCTATTTTTGCTTTATAAAATGCAGATTCGGATATTAGCATATTTATAGTGTTTAATAAATAACAATTAATTTGAGAATTAATAAAATCATTAATATTAGGGTATATTTTTGCTTTTTTAAAATCTATGTCATATAAATTATTATTGCTTTCATTTAAAGGAAATTTTTCAAACGAGAATTTAGACATTGGCAAAATTGTAAAATATTGATTAAAATTTTTATTGGAGTTAACTACGAAATTGACTTCTTCGTAATTTTCATATTCATACAAGATCATTACTATTTTGGTTAATTTTTCTGCTAAATTTTCAATATTTGAATTTTCATAAGATTGCAATACTTGTAGGTTATTATATTGACCAAATTTAATTGCATTTTTGCCAATTAAAATGAACTCATTATTAGATTTTTTTATATTACTTAGAATAGTTTTTTCATATCTTGAATATGAGTCAGTGCTATATTTTTGTTCTTCTGTTAAATAAATTCACAATATGTTTCTATTTATTTTCCTAAATAAATTAAACCTATGGCGACTTTCTCTCCTCAACATATCATTCTTAATATTGTAGATTTCTTTTAAATGCAAAATTATTTGCCTTGATACCAATGCATTTTTATTAAAAAAGCTTAATCTTTGGTTAAGTTTCATAATATTTATTAAATAAATATTTTTTTCATTATTTACTTTTAATGAAATATTGTTTAAACTTTTGCGTTTATTCTCTAAATCTTTTAGTTGCATGTTATTGTTCCTTTGTAACAACTGCTACTACTTTTTCACTGAATAATAAAACTAAATCATTTTTTAATTCTGAATGAAATAAAATTACATGTGGAAATATTTTAATTGATTTTTTAAGGGCCAAAAGTTTTTTTATTTCAGCATTATATGAATTCAAAAATTCTTGGTATTTTATATCGCTAGAAGTGGTTAAATCAATTGCGTTATTGGCAGAATAATTTTTTGTTGTAATTATAGGTACTAATACCTCTTGCGAGAATTTCAAATTCAAATTAAAAGTAATATTAAAATCCTTTAGTAAAATGTCATATTTATTTTTAAAAGCCAAATCAATTTTGCTTATAAAATCTAAATAAATAGGAGAATTAAATCCTAGGTTGTTATTAAACAATACTGTTGATATCATTTCATCAAAAGTAATTAAATCTTGTGAGGACTCGTGTTCTTTATATTCTTTAAATACATCAAAATAACTCAATAATGGATTATTGTCTTTCTTTTTCATATTCATTCTCCTTATTTAATTTTCTTATATAATAAATTGCCAAAATAAATTTAAGCAATAAAGATAATGCCATAATAAATGCAATGCTAAAGTTTAAAAGCAAGATAATTTGAATAATATTCATTCTACTTTTAATTATTGAAAATAAATAATTTTCATTGCCATCAGCAGTTAAAATATAATTAAATCCAATAATTGCAAAGATTAAAATTGAAGAGATTATTGCTAGTTTAATTATTATTAATAATAGCAATGAAATATTCTTGTTTTGTTTAGCAAACACAAATAATATTACTATTTGTGTTGTTGCCGTTACAGCAACGTTGATTAAAGAAACAATGCTATTGGTGTTAAATAATGTAGTCAATAGCAAACTTAACGCAAGCACTATTTGAACAATTGCTAAATTCAATCACGATCTAAATGAACTTTTCATTTCAAAACGTTTTTGAAATGAAACAACTATATATGCAATAAATACCACAATTGCAAAAACGCTTTGAAAAATGTAAATTCATGTTGCTTTTGAAGAACCTATTGAAATGTTTTCATTATTATGCTTATATGCAAAAATTCTTAGAATTCAAATAAATACAGCCCCAAATAATACTAATAAAAATAGTAATTGATTTTTTAAATAGAACTTATCATATTTTTTATTTATCAATAAAGCAAAGAATTTAATATTAGATAATACTATAAAAGTAATTAAAACTAAAAATGTCAATACAATAATCACAAAATTGCTAGCAGATTTAATTGTTAAAATATCTCTAATTGTTTCATAAAATTTATTGCCTACAAACAATATGTCTTTATTAATTCCTAAATTAATTCAAACAAATGATATTGCAACAAATAATATTGTTAAGATTCCTTGAAATGTTAAAGAAATTATTAAAATATTTTTATTCTTATAAAGCAATGGATTTGTTTTTCTTTGAATTAAAAAGTTAAATATTTCATAGCTAAAATTAATAATATATAAAGCAATCAAAATAAATGATATTGCAAATAATTTGCTTGGAAGAATTTTAAAATAGCTAAAAAATAATGTATAAGAAGTTAATGAAAGCAATAGATATAAGAAGTAAAATGGAATATATTTTTTAATAGTAACCTTATGTCAATATAAATTAGCAAAGCCTTTTACTAAACTATAAAATAAGACAAAGGCTAAAATTAAGAATCTATAAATTATTAAAAAGTTTAATTGTTGATTTCTTGGACTTGAAAAGTCAAAAAATGAATTTAGTGAATTAGAAAGCAACATTTTATTAGCAAAAAGTGTCTTTTCACTAAAAATTAATAAAGTTGTAATAAATACAAGACTAATTACAATCAAAAGGTATTTCAACACCTTATTGAAAATGTTGTTTTTCTGCTGATTTTTTATTGAATATTCATCATCATAAATCAACTTTTGATTTATATTGTTCGAATCGATTTGTTTTTGTTTCATCTTGTTATTTTCCTATTAAATAAATTCTCCGTCAGAATCAGAAGAAGAAGAGGTTTTTACACTATATCCCAAACCTTCTAATTGAGATTTTAAAGCTGAGGCATTATTATCAACAATTATTTCTTTTTTCAATTGTTCTGCAAACATATATAAACGCGAAAGGTTGTCAATTCCTTCTGAATTTAGTAATGAAGTTCCCGTAATTCTTACTTTTGTTGTTAAATTTCCATTGCTAAATAAAATCTTGCTCTTTTGACCAGGTTCGCCTGAAACAATATGTCTATTAAAACTTGCTTCATTTAATTCTTCACCTGATATGTTGAATGCTTCACTGTTATTATATAAAGTAAGTCTTCTTATCTTTCTTGATTTTGAATTACTTGGTTTTAATTTATCATGAAATTCTAATCCCCTCAAAGAACGTATCTTGGGTATTCTAGTAAAATCTAGGCCTTGTGGATAACTATTGCCATCTTCTTTATGATCTGGTTCTAAGCCCGGACCAAATCCACCTTCAAATATTGGTTCATTATTTCTAACTCAATAAACCATTCTCATTCCATCGTTAATTCTCTTTAATTTTTCTTGTAAAGTAGAAGCATTTTCATTGTAATCAACAGGATCAAAGGCCAATGTATCAAATGTTATTCTTGTGGCGATATCAGCACCTTGTTTATAGTCTCAAGAAACATTGTAATCGTTTGAGTTTATTCATTCAACTTTTCTTAATGCATTAGGGTTAATTGATCATTTATCCAATAATGAATTTCCTAAAGTATAAATTCCTAGTTCCTTTATGTGTTTATTTTCTAATGCAATTAAAGAAGAAGTGTTTGAAGAACCTGCTGAGAAAAATAGTTCTAGTAGTTGAATTTCATCAGGAATTGCCTTTAAAATATCCATAAATTTTTGGCTAGAGTCAGATTTTCCCATATTTCTAATTCTATAACCAGTAATATTGACCTTTTTAGCCTTTAAATCTTCAATTAATTTTTTAGTTTTTGCATAACCATCTGGGTTGTCAGCATCAATATCAACGATATAACCTTCATTGATCTTTCCATCTTCTTTTTTTTCTCTAGTATATTTATTGACAGTTATACCATCGCCATCTTTAATACCATATTGTTGAAATTTCTTAGTTACATTAGTTTTCTTTCAACCAGGATAGTCGCCATTAGCAAGACCATATGGGCTTCTATTTTCAATACTATCATATCCAAAAACTCTTCTATTTTTATTGTCATTTATTATTCTATTTGCAACACCGTTATAGCCTTTGGCTGGAGAATATGCATATGAATCTAGCTCACCTTTTTCATTTACATAGGCGTTATCTTGATCTAATGCAAGGCCTTCAGCTAATTGCTTTTCAGCATTAGAAGTAATCTTTGTAAATTTAGAGAAATCTAAATGAGAATACAATCAAATATATTTGAAGTGTTTCCTACCTTCAAATTTAGGCTTCATTTCTGGATACTTTGCCTTGCCTTCTGCTGTTAAGAAATTAACAACATTTTCTGAATCAAATAATCTTTGGAACTTTAATAATTGGTTTGCGGTGTAACTTAATGAACCACGATCAAATTCTCTTTTAAGATAATCTTCGGCTTCATCTATTCAATTTGCCCCTCAACTTTTTTCATAATCTTCAAGATCTTTTTTGAAGTATTTTTCATCTCTTTTTAAACGTTGAGTAGCCGCTTGGGCTGCTGCTTGTTTCAATTCTTCAGTAACCTTAACTTTTTTAATTTTTGGAGCACTATCATTGATGTATGGGTTTAAATTAGTTAGATGATTTAAGATATCATATTGATTATATTCACGCTTCTTTTGATCTTCGACTTCCGCTTCAACTTTGACACCGTTTAGAATAATTTCAGTTGTTCTAGTTGAAGGCTTTGATGAAGGAGGAGTAGGCTTGGGTTCAGGTTTGGGTTTTGGTTCTGGCTTTGGAGCTGGAGGGGTTGGTTGAGGTTTTGGTTCTGGTTTGGGTTCGGGTTTTGGCTCTGGTTTTGGAGCTGGCGGAACTGGGATTGGTTCAGGTTCTAGTTTAGGGGTTGGAATTGGTTGTGGCTTTTCTAATTCTTTTAAGTTGTTATCCTTTATTGAAGGCAACGCATTTTTATAATCTAGGTTGCTTTTATTTATAAAATCATTTCCTGTTGCTGAGCTGGTGGTATTAAAACGAGAATAATTTTGCGAACTTGTACTATAAAATATAGCCCCAGCAATTATTGAAGCTCCTATAATAACACCTGCTGTCATTAAAATTAATTTGTTTTTTCTTTTCTTTAATAAGTTTTTCATTTCACCACTTTTTTTAAGTTTTATTATTGATTATTTTTTGTTTTCTATAAATTTAAATTCATTAGGAACTTTATCATCACCTAAGCCATCAGGGAATAAAGCTGTTTTAAAATCACTATTATCTTTATATAGTTCTTTTAATGTTTGTCTAAATGAATTTTTTTGGTCTTTGCCGCCACCATAAATTAGATCATATTGAGGCAAATTGTAATTTCCATACAAGCCTTTGTAGTCAAATCCTTCCGATCTAAAAGCAGCTGCTAAGCCGGTTTTTGCGCCTAAATTCGATAAATAAAATATTCCAACAAGTTCGTTTTTTTGATTTCTTAAGCTTGTACCAGAAGCACCTCCAACTGGAGCAAAATGTTTAGGCATATAATTTAGCCCCATACGCATATATGTTTTGCCATCGCTACTTTCATAGAATGAAAATACTGATTTTTGATATTTTTGGCCATTTATTTCTTCTTGAACTTGCTTGGTTCCACCTGGTTGTGCTAAAAACACATCAGAAATACCAGGTTTATCAATGAATGTTCTATATCCTAAACTATAAGATAAGTAATTTCCTCTTTCTGTTTTTGATGGAGGAAATGAAGGGGTTTCATTTTCACCAACTGCTAATTGATCATAAAATCTATAATCCGAATTTGTTCATAAACTGTAATTAAATTCATCTCTTCTTTTAATTTGGTCATCATCTACATATCTTCTTAAAAAGAAATCTTGGGTTGAATTTGGATAACCTAATGCAAAAAGTTCATCATAATTCTTTCATCATTCCTTGTTTTCTTTATTTGCTTTTAAAGGAACATCAATTTTTTGATAATTTGATAAATATGATTTCGACAAGAATTTAATTTGTTTTGCTTTATTTTGAGCATAATTACTTGTTAAGGCTTTTGCTAATTCGTGGGCATCTAAATTGCTAAATTTTTGTGTAATATTGCTATTTTCACTTAGATCATTTTTAGAAACGCTTTGCAATGTCAATTTTTCAAAATCAACTTCAAAGACTGCAAAATCAGCATATTCTTCAACATCTTTATACTTTTCTTTTTGCGATTTAATTAAATAATCAGAAGGTTTTGAATTTAAGAAATCTCTTGCAGTATAAACTGTCTTTATTCCATTAGAAACATTAGTAGAATCTTTACCAACAAATGAAAATCTATATGTATTTTCATCAAGATTTGCTAAACGCAATGTTGTTTTAATTTTTGCACTATCCATCAATTTTAGAAGTGTAAATGAAGTTGTAAGTTGTTTCAATGCATCTGCAACGTGTAAATTTGTTGCAAAATATCATTTTGTTGGATATTTTCCATCACTTCTTTGTTGATAATCCATGATTCAAGTTGTTCCAGCTGTATTAGTAAATTTTCTTTCTTCACCTTTAATAGGAGTTAAGAATCTTATTCCAAATGTTTGAGAAGCAATGTCACGATAATGTTCATTTGGCAATGTTCTTGCTAAACCTGTAATTTTATATGGATCTCTACCAAATGCATCTGTAGGTGAAGGCCCTTGTCCTCCTGGAGTTTCATTAATAGCAAGGCCTTCTACATTACCATTTGAATCGTAGCTTGGCAATGTAAAACCTTTTCTTGCAGCTGATTCATACGAATCTTGACTAATTTCTTTTGCCTTAGCATCAAATTTTTGAATGTTAGATTGTGAAGCTTCCAAATTTGGTCTAATTTCTTTTATTGATTTTTGTTCTTGTTGACGAGTTAAAATGTCTAAATATTTTTTATTATCTAGCTCATATCTTTTTGTTTGATTTCCTTCAAGATATTGTTCCAACTCACTTTTATTCAATCTTAAAGCATCAGAACCTGTATTAGGAATTGTTCCATTTTCATCAAAACCATAAGGATTTGTTTTAAATTTTCCTACAGTTATTGTTTCAATAAATGTTTTGTTTGTCTTTTTGTCTTTAATAGTAATATTAAAATTCAATTGACCGGTTTTGTTTGCAACTGATCAATCATCAGAATTGGTTGAAGTTTGTACGTATAAAACATTGACTATTTTTTTAATTTCAATTTCATCATTAAATTTTTTTTGTTTAAACTCAACACCTTGATTGAAATTTCTAACAATTTGAGATGGAAGCAAATCTTGTGAATTTTGATTTGAAAAAGCCTTGAATTTCAAATCAAATAATTGACTTGGATCATTTAATGCTAATTTTGAAACTTCAGAAATTATTTTTTTATATTCGATAGTTTTTTGTTCTTCAGCTGATAGTTCATCTGTTGAATTATTATTTTTGTTGTCATTGCCTTTTGAATCTTGATTATTTTGGGGTTTTTCATCAGGATTCTTCTTTTCGTCATTTTGATTATTATTAGTCTTTTTATTTGTATTTTTACATGAAATAGCTACAGCAGACATACTAGAAAGTGATGTCGCTGCTATTAATAATGATATTATTAATTTAGATTTTTTCAACCTTATACCTTCCTTGTTTTTTAATTTTAAACTTGAAAAATAGGAACTAAATTTCCTTTGATTTTTTATTGATTTCCCTATCTTAAAAAAACAAATATTTCCATTTTTCGCAATAATTTTACTATATTAAATTTAAAAAAAAAAAAAAATACTTCAATGTTTAAAAAAATTACTTTAAACCTTTTAAAATCAATATTAAATTCTAGGTATCTAATTGAAATTAAGATAATAAATGCATATCAAGATATGCTTTAAAAAATGAAATTTTTCATTTTAATGTATAATTTAAAAATTAATATTTGAGGAGGCAACATGTTGGATGTTTGCAATTTAGCTAAAGTTTTTCCAGACAAAAAATTATTTACAAATGTTAACTTAAAATTCTTGCCTGGAAATGTATATGGAATTATTGGCGCTAATGGGGTTGGAAAATCCACTTTTTTAAAAATATTAAGTGGGGATATTGAAGCATCAGAAGGGCAAATAATTAAAGAAAAAAATGCTAGAATGAGTGTATTATCACAAAACCAAAATGAATTCGACGATTATATTGCAACTGATGTTGTAATAATGGGGAACAAAGAACTTTATCAAATTAATCTTGAAAAAACTAAAATTTATGAAGATCCAAATGCAACTATTCAAGATTATGAAAAAGCAAGCCATTTAGAAGAAAAATTTGGTGAAATGGGGGGTTGAAATGCTGAAAACGACGCCCAAACATTATTATCAAATTTAGGAATTGAACCTGAAAAATGAACTATGAAAATGTCGGATTTAAAAGCCAATGAAAAAGTAAAGGTTTTATTGGCAAAGGCTTTGTTTGGAAATCCTGATATTTTAGTAATGGATGAACCAACAAACAGATTGGATCTAAAAACAATTAAGTGATTAGAAAACTTTTTAATGGATTATCCAAACATTGTTATTGTTGTTTCTCACGATAGTGATTTCTTAGATGCAATTTGCACGCATATTATTGACATAGATTATTCTGAAGCTAAATTATTTATTGGAAATTATTCATTTTGAAAACAATCAAGCCAATTGTTAATAGAAATGCAACAAAAACAAAACCTTAAAAAAGAAGAAATTGCCCAAAAATTAAAAGATTTTATTGCCAGATTCTCTGCTAATGCCTCAAAATCAAAACAAGCTACAAGTAGAAAGAAATTACTTGATAAAATTGTCATTGATGAAATCAAACCATCATCTAGAAAATATCCTTTCATTCAATTTAACTTAAATAGAACTCCTGGAAAACAAATATTAACTGTTAAAAATTTAACTTATGTAAATGACAATGGCGACACTTTATTTGAAAATGTAAGTTTTACAATCCACCCCGGAGAAAAAATGGTTATTATTGGCAATGACGACATTGCCAAAACTAGATTGCTTGAAATACTTATGGGTAAGCGTAAACCAACATCAGGAACAGTTGAATGAGGAATAACTATTAAACCAAATTACTTCCCAAATAATAACCTAGAATATTTCAAAGAGCCAATGTCAATAATGGATTGAATTAGCAAATGGCCTCTAAATAATACAACTCAAGAAACGAAAGATAATAGTGATTCAAGAATGAGAGCATTTTTAGGAAGAATGTTATTTTCAAATGATAGCGTATTTAAGAATGTTAATGTAACTAGCGGTGGTGAAAAAGTTAGATTAATGATGGCTAAACTAATGTTAGAAGAAAGTAACTTTTTAATCTTCGACCAACCTCTTGACCACCTTGATAGTGAATCAATTGATAGTTTAATTGAAGCAATTAAAAATTACAAAAGTAGTTGTATATTCACAACATACAATAGAGCAATGATAAAAGAATGTGCCTCAGTTATTTTAGAAATAAAACCTACTTCAAGTTATTTATTCTATGGAACCTTAGAAGAATATGAAGAAGCAATGGGATATTAAATAACTAATTAAAAAAACTCGTAATTAAAACTACGAGTTTTTATTTACTTTTTAAAGTTTAGCAGCTTATTTCTATAATATTCGTATTGTTTTCTACGTTGATCAATTTCAAGAGGAAGGCCAGATTGAATGTCTTTGGTGTATGTTTCAAGTTTGTCAAGGATTTCAACTATTTTATTTTGAATTGAAATGTGAGGGATTGGGATGAAAAAATTATCAATATCGATTTTATTTAATGCAGGAATACTACCTTTTGCAACTAATTCATTTGATATTTTTTCTTCATTATATTTAAGCCAATAGTATATATATTTAGTTAAAAAAGATGTTTCATTTTTACTTCTATACGGATAACATAATCCACCTGCTCAAAATTTATCATTCATATAATTTATAAAACCAGCATATGCTCCACGCGCTGCAATTGTTATAGCATTTCCTTCATTATTATATTGGTCATATGTCCCATATATTTCTTTGCCACTATTAATTACTTGATATTTGCCATTATTTAGTAATTTGTTTTTTTCTAATGTTTCCTTTTTTAAATTAATAGTTACCTCATTTATCCTCTTATATTCAATAATGTTAATTATCTTTTCATAAAGTTTATTTAATAATAATATGTAATTTTTACTTAATACCCCCCCCGCAAGGTCTTTAAAGTCTAACAACTTATCTCTATAATATTCGTATTGTTTTTTGCGTTGGTCAATTTCAAGAGGAAGGCCGGATTGAATGTCTTTGGTATATGTTTCAAGTTTGTCAAGGATTTCAACTATTTTGTTTTGGATTGAAATGTGAGGGATAGGAATTTCAATATTTTCCATAATATTGCTCATAAGTTTAGGATTTCCACTTGCCTTGTTTACAAATTTTTTTGTCTGCATAGATAAGCAAGTGCTTAAATATTTCACATTTAATTTCGATTTATTTAGAACACTCAATATTCCACACACATTAGTTATTGAAAATCTGCCAATTCTATAAAATACTGTTCCAGCATATGCTCCATCCGTAGTTCATGTTACATATTCACCATCAAAATCATATTTGTCAATTTTTCCTAAAATTCCATCATTTAAACTTTGAGATGAATAAACAGGGTATTCACCTTTATTTAATTTTATGAATTCTTTTGAGTAAACTTTTCCTCGCTTTATTTCACACAAGTTTTTAATTAAATCATATTCAACTATATTGACAATTTTGTCTCAAAGCTCATTTAAAAGCAGTAAATAATTTTTACTTAATACCCCCCCCGCAAGGTCTTTGAAGTCTAACAACTTATCTCTATAATATTCGTATTGTTTTCTACGCTGGTCAATTTCAAGAGGAAGGCCGGATTGAATGTCTTTGGTATATGTTTCAAGTTTGTCAAGAATTTCAACAATTTTATTTTGAATTGAAATGTGAGGGATGGGGATTTTGATATTCGTTATTGAAGACCAATGTCTTTTATATCCTAAAGAAGGTATATTAATATTATCTAATGAATAAAATAAAAATTTTGATGATACATTATTTTTTGTTTTATCTTTATTTAATAAAATTTTTAAACCATCACCACCTTGTGCAAAGGTATGATTTATAAATTTAATTTTTCTTGTATGATCACCAAACAAAATATATTCATTTGCTGGCAAAACAGCATTTTCATCATTTGTATACATTTTTTTATCATCGCCTTGATTAATAACTATATATTTTCCATTATTCATTGTTTCGTTAATAGGTATTTTCTTAGGGGGTATTATAATATCAAACAAATCTAATATTTCTCAATAATTAACTAAATTATTTAAAGAATCTGAAGTTGTTTTATTCATAATTTTGGCTATTAGATTTTTATTATTATCAAAGTCTAATAATTTATTTCTATAGTAATTATATTGTTTATTTCTATAGGTCAACTCGGTGGTCAACTCGGTGGTCAACTCGGTGGTCAACTCGGTGAACTTGTCTAAAATATTAACAATTTGATTTTGGATTGAAATATTAGGAATGGGGAATAATAAATTCCCAAGTTCCTCTTTTTTAATTAAAGGTAAATTTACGTTTTCATATCTTTGCTTTAAGATTTTTGAAAAGATAAAAAAATAATAAAACATAAACATTGGGTTTATTTTAGCCACAAATTTATCTTTAACAGTAAAAATTGAAAATTGTTGATTGCAAAAAAATGGTTCTAATACAATAGCATGTTCACCAATTGTTGCGGTAGTAGATAATATAATTGAATTTGCTTTAAAAATTTTTTTTATTCTTAAGACATTATTATCCACTTTAGTAATTGAGCTTTTTAATACTCGACCTTTTTGTCTAATATCTTCAAGTTTAAATCAATTTATATTTTCGTTTTTTCAATATTCGGAAAGATTTTTTTGGGGAGTAAAACCTCCAAATATGTCGCAGATATCCTGCATTTTTTTATATTCAACTCCATCAGGACATAGTTCCTTAATTAACTTTTCTATTTTACTCATAACTATTCCTCTAATTCGGCAACTATTTTGTCAATTTCACTTCTCAATGTTTCAATCTTGGAAACTGTTTCTTTTATTTGAGAGTTTAAGACATTAATATCTATTTTTTCTGAAGTGTCTTGTTTTTCAACGTAACTATTGACTGATAAATTATATGAATTGGACTTAATTTCTTCAACACTGGCTAATTTTGAAATATAAGAAATGTCTTTTCTTTGGCCATAATAATCAATAATATTGTTAATATTTTGACTAGTTAGCTTGTTGTTGTTTGTTACTTTTAAAAATTCTTGAGAGGCGTCAATAAATAAGATATTGTTGTCAATTTTTGATTTTTTCAATACCATTATGCATGTTGAAATACTTGTTCCAAAAAACAAGTTGCTTGGGAGTTGAATTATTGCATCTATGAAGTTATTTTCGACTAAATATTGTCTAATTTTTTGTTCAGCGCCCCCGCGATACATAATGCCCGGAAAGCAAACTATTGCAGCTACTCCATCAGTTGCAAGTCAAGAAAGGCTATGAAGTATAAAGGCAAAATCAGCTTTACTTTTTGGAGCTAATATTCCAGCAGGTGAAAATCTTTGGTCATTTATTAATAAAGGGTTGTCTTCACCTTCTCATTTAATTGAATAAGGAGGATTTGAAACAATAACATCAAATGGTTCTTTGCGTTGATGTTCAGGGCTTGGAGATAGCAATGTGTCGCCGTTGTAAATATTGAATTTATCAAATCCTATATCATGCAAAAACATATTTATTCTACATAAGTTATATGTTGTTAAATTGACTTCTTGGCCATAAAATCCATCCTTGATGTTTTCTTTGCCAAGAACCTTGATTGTTTGTAAAAGTAAAGAACCCGATCCACATGCAGGGTCATATACTCTATTAACTTTTTTCTTATTAAAAACTGCTATTTTAGTAAGTAATTCAGAAACTTCTTGGGGAGTAAAATATTCACCCCCTGATTTTCCAGCATTTGATGCATACATTGACATCAAATATTCATAGGCATCACCAAAGGCATCTATTGAATTATCTTGATAATTGCCTAATTTCATGGCTGCTATTTCTTGCAATATTGCTGCTAATTTTTTATTTCTTTCATCAACACTACGACCTAATTTTTGAGAATTAACATCAACGTCATTAAATAGACCTGAAAAATCATTTTCACTTTGTTGTCCTTTTGCTGATGATTCAATATTTTTAAAAATATTGTCCAACGTTTCATTTAAACAATTGTTTGTTGAAGCATTTTTAACAACATTAATAAACAATTCAGAAGGTAAAATAAAAAATCCTTTTTCATTGATTAAATCATCTTTGCCCGTCAATGTTTCTTCATCTGATAAAGTTGTATAATCAAAATCTTCAATTCCTGCTTGACGTTGGCGATTGTTTGCATATGTTGCAATATTTTCAGAAATATATCTATAAAACATAATTCCTAAAACATATTGTTTAAAATCTCAACCATCAATTGAACCGCGCAATCTATTTGCTATATCTCAAATCTTTTTATGTAATTCGTCACGTTCTTGATTATTAATAGCCATAATTTTCCCCCAGTGTAATAAATGACTTTTATATATAAATTTTACAAAAATAACCTCAAAAAAGGTTCATTATCACTTTAAATTCAAAAAACCTTTTGAATCTTAAATATACGAATGACAATGTTTCTACAAATTAAAATACAAAAAAACTCGCAATAAATGCAAGTTTTTTTTAATATATCAAACATTTTTTTAGTCAATAGGTTTTATTGCTTGAAGAACTAATTTAGCAAGTTGATTGCCACCCAAAACTTCAATAACAGCTAATCCAAAATCAAAAACAGCAGCCGCGCATTTTCCTGTTATTAATTTTTTATCTGGGCTTATTGATACTAATTCTTTACTACGATTAGATATTTCTTTTACACTGTCGTTTATTGGAAATGAACTAAATTTAGTTTTACTATCAATAAACTTATTTTCATATAAAACATTGGGAGCATCGCATATTGCTATAATTAATTTGTTTTGACTTTTAAATTTAGCTATTAAGTTTAAAAATGTTTGGTTCTTTCTAATACTTTTAGCAGCGGGTCCTCCAGGAATAAATAAAACATCATATTCATCAACATTTATATTTGAAGTTGTTTGCAATTTTACAATTTTATGTGCACCAGTTGCTTCAATTCCTAGAGGATTGTAAAATGTGGTCTTATTAAATAAATTATTTGCTCTATGAAATAAATCTAATGTTCCTGTTAATTCAGTATCTTCAAATCCTTCTTCAACAATTACTAATAAATTCATAATTTTCACCTCTTGTTTGATTATATACTTTTAAACAAAAAAGAATAGATAGTTTTAACTAAAAATCTTTTATTTTGTTAAATAAATTATTTTAAATTTAAAAAATTCAAGTGAATATTTTTAACACTTGAATTTTTATATTTTTTTAACATTACTTTTTTTCTTTGAATATATATTTATATTCAAAAATATAGTCATCTTCAAAACCTTCAATCTTTTGTTGATGTTCTTTAGATTTTGAATATTTTAAGCTAAAATCCATTTCAATCGTTTTAGGATTTGAATTCATATGAATTGTTAATTTATGAATATTAATATATAAACCAGGCAATTCTTTTCAATCTTTTGCAGCACCTAATTCAAAGTTTTTAATTGTGATTTCACCGCTTTTTAAATTTGCATAAACTTTAGTTTTTTTAAAGTTCGAATATCCTGAATTATATTTAAAGAATGCTTCTTTTAAATCAACATTTTCATTATCAATAAATTTTGCTTCGAACAAAACTTTTCTAGGAATCAAGAAAAAAATAAATTTTTTGGAATTGGATTCTTCATCTTCTAAGACTAATCTATCTACAATTCAATAGTCTTTTCAATATTCAAATCATTTTGACTTTGGTCCTATAGCAATTTCTAAAAAATCATTACGTCAAGGACCTGCTATACTTTTATTGCTTTGCAATACTGAATTATAAGATTTGGCACTGAATAAATTTTCTATATAAAAATGTGGATTACTATTTCCAATAATGTTTAAAACTTTTTTATGTTGTTCTTGTTCATTATAGCAAGATGTAACACATAATGGCAAAATTGGTAATGTTGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATCATTTATTTAAGACTCCATTTTAAAATATTTATTTTTATATTTTTTATAAAAAACATAAGGATCTTTATTTAGTATTCTCTTCAAAAATAAGTTTAAATTTTGAATATGGACCAGTTTTAAAATCATATTCGCTTTTTGGGTGTTGAATATATCACAAATGAACATCAAGTTCAAGTCTTTTTTTGTTTAGACTGCAATCTAACATTAATTGTTTAATGTAAAAAAAGCGCAACTCGGGTTTATTATTCTTATCATCTAATTTATGCACCGGGGCCATATATTTATAAATTCATATTTTTTTATTTTTTAAATTTGTTGAAATTTTTCTAGGCTGTTCATTAAACGCAACATTAAGTTTTTCACTATAAAAGTCTGCTAAATTAAAATCTTCGCTTGAAATAAATCCTGTTTCTAATAATACTTTTTTAGGAATTAAAAACAAAATGGAATATGCATAAAAATCTATCTTTCCAAAAACACAATAATCTTTTTCATATTTATCAAAATTCTTTTTTGATGTTATTACAATTTTAAAAAAATCATTACGCCATGGGCCAGAAATAGTTGCGTCGTTATTTAAAACTGATTCTTCTTCGCTATTTAAAATATCAACCATATTAAAAACTATTTCTTTATTGCCAATAATTGTTTCGGCATTTTTTAATTCAGTTTCTCTATTATTGCAAGAAGTTACTAATAAGGGCAATATTGGCAATGCAAAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATTATTTAAAATCCTTTCCCGAATATTTAATTCCCTTTTTGATAAAATGTTTCTTTGTTTTATAAACCTTTCAGTTTGGTTTCATGCCCAATAAAAATAATTCGCCAGAAAGACTTGAAGAAACAATTACTTGAGAATATCCTCCCCATCCATAATGGCAAAGAGGTTTATTTCCATTGTCATACATTCCATAAGCAACAATCGCATGACCAGTTTTTCCTTTTTTTATTGGATTAGGCATATATGTTCCAAATATTAACCAAGGATAACCATCTCTAATTCATTTTCAAGGTTTTATTCACCCTGCAGATCTTCGATAAACATAAGCAGGTCAAAAGTTTGGCTAATCATCTTTTTTTGTTTACACCTTCATTATAAAAAAACAAAGAAAATAAGATATTTTTTTATTTAAAATAATGATGAAATTAGTTAAAAAAAATTAATAGTCGGTTATTTTTTTATTTTTTAATATTAAATTTAGCGCAACCTAACTAGTTGATAAAAATGATCATCAATTTATTTATAAATAATTATTTAGTTGAATTTATATGCCTCTATTTTAAGTTCTACAATTTTCTTAAAGTTATATTTAATGTGACAAAAAACCTATGCAAAAGTTATAATTTCGCTTTTTGCATAGGTTTAGGCTTATTCTAATTTATTATCTATTTTTTCTAATGCTTTTATTGTTGTTTTAAAAGAAACTTTTGCAACCTCGCGCAATGTCTTTTGACCATATTTCAAAATAAAATTTGCTGCCAAATCAATAATTTTATTGCTTGCACCAAGTTTGAATTCAAAATTATATTTTTTGTTTTGTTGTTGCATTCAATCTATTAAAAATTTTCTTGATATTATTGAAGCTGCTGCAACAGCCAGGGATTTTTCTTCGCCTTTTGTTTCAATAATTATTTCATTTTGAGGTTTTTTAAAATCTAGTCAAGAAATTGCTTGCAGTTTTTTGAAATGCTTTTGTAACACTTCTAATGAAGTTGTATATTGATCAATAAAAACATCAATGTTCTGATTTAATTTTTTTGTTAATATATTTATTGAATTACTATGAATTAATGTTTTTATTTCGTTGTTATTAATACCTGCATCAATTAATTTATTATAACCACTTTGACTAAGGTAAGTTTTTTGATAATAAACTAAATTTTCAATTTGATTAAAAATTTCAATTATTTTTTCATGAAATAACCTCAAAAAATTTGTTATCACTTTAAATTTAAAAAATTCAAGTGGAATATTTTTAACACTTGAATTTTTATATTTTTTAACATTTACTTTTTTTCTTTGAATATATATTTATATTCAAAAATATAGTCATCTTCAAAACCTTCAATCTTTTGTTGATGTTCTTTAGATTTTGAATATTTTAAGCTAAAATCCATTTCAATTGTTTTAGGATCTGAATTCATATGAATTGTTAATTTGTGAATATTAATATATAAACCAGGCATTTCTTTTCAATCTTTTGCAGCACCTAATTCAAAATTTTTAATTGTGATATCACCACTTTTTAAATTTGCATAAATTTTAGTTTTAGAGTTTCAATATAATGAATTATATTTAAAGAATGCTTCTTTTAAATCAACATTTTCATTATCAATAAATTTTGCTTCGAGTAAAACTTTTTTAGGAATCAAGAAAAAAATACAATTAAATTCTTTATCTTCTAAGGTGAATCAATCTAAAATTCAATAGTCTTTTCAATATTCAAATCATTTTGAATCTGGCCCTATAGCAATTTCTAAAAAATCATTACGTCAAGGACCTGCTATACTTTTATTGCTTTGCAATACTGAATTATATGATTTGGCACTGAATAAATTTTCTATATAAAAATGTGGATTACTATTTCCAATAATGTTTAAAACTTTTTTATGTTGTTCTTGTTCATTATAGCAAGATGTAACACATAATGGCAAAATTGGTAATGTTGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATCATTTATTTAAGACTCCATTTTAAAATATTTATTTTTATATTTTTTATAAAAAACATAAGGATCTTTATTTAGTATTCTCTTCAAAAATAAGTTTAAATTTTGAATATGGACCAGTTTTAAAATCATATTTGCTTTTTGGGTGTTGAATATATCATAAATGAACATCAAGTTCAAGTCTTTTTTTGTTTAGATTGCAATCTAACGTTAATTGTTTAATGTAAAAGCGCAACCTGGGTTCACTATTCTTATCATCTAATTTATGCACCGGGGCCATATATTTATAAATTCATATTTTTTTATTTTTTAAATTTGTTGAAATTTTTCTAGGTTGTTCATTAAACACGAAATTAAGGTTTTTACTATAAAAGTCTGCTAAATTAAAATCTTCGCTTGAAATAAATCCTGTTTCTAATAATACTTTTTTAGGAATTAAAAACAAAATGGAATATGCATAAAAATCTATCTTTCCAAAAACACAATAATCTTTTTCATATTTATCAAAATTCTTTTTTGATGTTATTACAATTTTAAAAAAATCATTACGCCATGGGCCAGAAATAGTTGCGTCGTTATTTAAAACTGATTCTTCTTCGCTATTTAAAATATCAACCATATTAAAAACTATTTCTTTATTGCCAATAATTGTTTCGGCATTTTTTAATTCAGTTTCTCTATTATTGCAAGAGGTTACAAATAAGGGCAATATTGGCAATGCAGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATTATTTAAAATCCTTTCCCGAATATTTAATTCCCTTTTTGATAAAATGTTTCCTTGTTTTATAAACCTTTCCATTTGGTTTCATGCCCAATAAAATAATTCGCCAGAAAGACTCGAAGAAACAATAACTTGAGAATATCCTCCCCATCCATAATGGCAAAGAGGTTTATTTCCATTGTCATACATTCCATAAGCAACAATTGCATGACCAATTTTTCCTTTTTTTATTGGATTAGGCATATATGTCCCAAATATTAAACAAGGATAACCATCTCTAATTCATTTTCAAGGTTTTATTCACCCTGCAGATCTTCGATAAACATAAACAGGTCAAAAGTTTGGCTAATCATCTTTTTTTGTTTACACCTTCATTATAAAAAAACAAAGAAAATAAGATATTTTTTTATTTAAAATAATGATGAAATTAGTTAAAAAAATTAATAGTCGGTTATTTTTTTATTTTTTAATATTAAATTTAGTGCAACCTAACTAGTTGATAAAAATGATCATCAATTTATTTTTAAATAATTGTTTAGTTGAATTTATATGCCTCTATTTTAAGTTCTACAATTGTCTTAAAGTTATATTTAATGTGACAAAAAACCTATGCAAAAAGTTATAATTTCGCTTTTTGCATAGGTTTAGGCTTATTCTAATTTATTATCTATTTTTTCTAATGCTTTTATTGTTGTTTTAAAAGAAACTTTTGCAACTTCGTGCAATGTCTTTTGACCATATTTCAAAATAAAATTTGCTGCCAAATCAATAATTTTATTGCTTGCACCAAGTTTGAATTCAAAATTATATTTTTTATTTTGTTGTTGCATTCAATCTATTAAAAATTTTCTTGATATTATTGAAGCTGCTGCAACAGCCAGGGATTTTTCTTCGCCTTTTGTTTCAATAATTATTTCATTTTGAGGTTTTTTAAAATCTAGTCAAGAAATTGCTTGCAATTTTTTGAAATGCTTTTGCAACATTTCTAATGAAGTTGTATATTGATCAATAAAAACATCAATGTTCTGATTTAATTTTTTTGTTAATATATTTATTGAATTACTATGAATTAATGTTTTTATTTCGTTGTTATTAATACCTGCATCAATTAATTTATTATAACCACTTTGACTAAGGCAAGTTTTTTGATAATAAACTAAATTTTCAATTTGATTAAAAATTTCAATTATTCTTTTATCAGAAAGTTTTTTGCTGTCTTTAACACCAATTTGTTTTAGTTTTTCAATGTTATCTTTTGGTATATAACAGGCAACTGAAATTACGGGGCTGAAATAATCACCTACTCCCGTTTCATCAACGCCAATTAATTCATCTAAATTTTTATTCAATTTCTTCTTCTTTACTAAAAAAGTTATCTGCACCTACAGTTTTAATATTATTTTTTTGTTCGGCCTTTTGTTTTTTACCTAATTTAATGTATTTTTCTGGGTTTTTTAGGAATTGATCAATAAAGTATCCAACTGCGCCTTCTTTGTTTGTTTGCTTCATAACAACTGTTGACATCTTTTTAATTAAGGGTTCAGCATTTGCAGGTGATACACAAACATTAGCAACATTATACATGCTTAAGTCATTATATGAATCTCCCATTGCAACAGTATCATCAATGTCAATGTTGTAATATCTCATAATTAGTGAAATAACTTTTCCTTTATCGATACCTATTGAAGTAATATCAAATACTGGGCTTAATCCTTCACCTTTTGATCAAGAAGAAAATTCTCCTAAATCTCCATAACGTCTTTTTAAATATGTCAATAATTCCAAGACATCAGTATCAGGTTTAACATCGAATACTATTCCGGTTGGTTTTAGTGGAATTTTTTCTAGGTTAATACATTCTCTAAATTTTGTTGCTTGGTTAAATCCAAACACTCTTTCAAGGTTAGGATCTCTATGCATTAATTGAACCCAGTCAGGGCCTTCTATTGCATAATTAGTAATTTCTTTTTTTACTTTTTCATCGCCTAAAATATAAAGAACTTCATTTAAGTCCAAATATGTAATAGCAGGTATAAAGAATGGGTCAGCAGGGTTGTGGATGTGAGCACCATTGTAATTACCTACAATTGCATTAAGTCCAAGTTTTTCATACACAGGCAATGTACTTCTTCAAGGTCTTCCTGTAATTATTGAAACAATGTGTCCTTGGGCAACAGCTTTTTTAATTGCTTCTTCTGTTTTTGGATGAACTGTGCCATTGGCTGAATCTGCTAATAGTGTTCCATCTAGATCAATAGCAAATAAAAATCTTCTTTGTTTTGATTCTTTGTTGTACGCCATAATTTCCTTTCATAATATATAAATATTTTACAATATTTTTTATATCTTCTACATTTTGAACAAAATTACTAAAAAATGTGTCTCAATTTTCAATGTTTTATTGCAAAAATTTACATGGAATCAATTTATTGCAAATTAAAAAAAACTTCAAGTTTTTTTACTTGGAGTTTTTTAAAAATTTATTTATTTTATTTTTGTTCCAAATATACGGTCACCAGCATCTCCCAAACCAGGCAATATATAATTGTGTTCATTCAATTTTTCATCTTTTGAAGCTAAATATATATCAACATTAGGAAATTGTTTTGTTACTTTATCAATACCTTGTTGAACGCCTACTAAACATACTAATTTAATATTGTTAAAGCCTAACTTATCCAATTTTGCTATTGCATCGCATGCAGATGAGCCGGTTGCAAGCATAGGGTCAACAATAATAATATAGCTATCATGTGGTACTTCTGGAATTTTAAAGAAATATTCAACAACTTCGTTTGTTTCTTCGTTTCTATACATTCCAATATGACCAACTCTAGCTTGGGGAACCAAATTCATAATACCATTCAACATTCCAAGCCCCGCACGTAAAATAGGAATAATTACAATTTCTCTATCAAGAGTTTCGCCTTCATATTTAACATTCATTGGTGTTGTTATTGATATTTTCTTAGTTTGATAGTCCCTCAATGTTTCATATACCATCAACGAAGCAATCTCGTTCAAATTATCTCTAAAATCTTTGTAAGATGTTTCTTTTGAACGCATCGTTGTTAATTTAGCGTTAATTAAAGGATGCTGAAAAATTTTTACCATATATTTGTGTTATCAACTTTCTTTTTATTATTTTTCTAATTTAAATAATTCTTCCATAACTGGAACTGATTTAGACATACCCAAACGGTTTGCACCTAATCTAATCATTTCTTGGGCATCCGCTAGATTCTTAATACCACCTGATGCTTTAATTAAAAGCTTGTCACCACAGGTTTCCTTCATAGTTTGAATGTCTTCAAATGAAGCTCCTCTATATGAAAATCCGGTTGATGTCTTAATAAAATCGGCGCCAGATTGCATTACTAATTCAGTAATTTTTATTAATTCAGCTTTTGTTAACAATGCTGTTTCAACTATAACTTTTAAAGTTTTGCCATTGCATGCTTCTTTTATTGCTTTGATTTCGTTTAATACGTATTCATATTGTCCATCTTTAAATCTTCCAACATTGATTACCATATCAATTTCGTCAGCACCATGTTCAACAGCAATTTTTGTTTCATATACTTTAACACTTGTTGCATTATAGCCTAGTGGAAAACCAATAACTGTGCAAACAAGCACATCACTGTTTTTTAATGCTTCTTTAGCATACTTAACATATGATGGTGCAATACATACTGATTTAAAATCATATTTAATTGCTTCTTGAATTAATTTATCAATGTCTTTGCTTGTTGCAGAAGGTGATAAATTTGTGTGGTCAATATATTTGTTTAATTCTGTCATATTCTTCCTTAAGTTAATTTAATTAATTACATTAATTTAGCAAAAACCGTTTGAATGTCATGCTTGCTTTCGCTAAATTCTATTGCAGACAACAATTCTTTTTGAAGTTCTGGATTTATAGGTTTTGACGAATACATTGTAAATAATACATCTCCTTTATTTACATATTCATTTGAACTCTTATTCAAATAAATTCCTGCTTCATAGTCGATAGAATCTTCTTTTTTAGATCTACCTGCTCCCAATTTCATTGCTACTAATCCAAAATCAATTGCAGATTTTATTTTTAAATATCCACTTTGGCTTGCAATAATTTCTTGTTTATATTGAGGATTAAATCATTTTGAATCTTTTTCAAAAACCTTGATGTTTCCGCCTTGTGCTGAAATTCATTCCAAAAATTTGTTATATGCTTTTTTATTATTGATTACTTCATCAATCATTTTTCTTGCTTCAACTTCATCTTTAGCTTTTTGGGCTAAAACTAATAACGTTGAACCTGATGAGTAAATAATTTCTGTAAAATCTTTAGGTCCGTGTCCATTTAATGTATCTATTGCTTCTAAAACTTCAATTTTATTTCCAATAGTTTTTCCTAAAGGTTGTTGCATATTGGTTATTTCAACGGCAATCTTTCTATTTAATTTCTTGCCAATTTCAATCATCAATTTTCCAAGTTTTTTTGCTTCATTAATATCTTTCATAAATGCGCCATTGCCACATTTAACATCCAATAAAATGCAGTTTGAACCGGTTGCAAGTTTTTTAGACATAATTGATGAGGCGATCAATGGAATTGATTGAACGGTGCCAGTTACATCACGTAATGCATAGATTTTTTTATCTGCAGGCACAAGTTTTTGATTTTGTCCAACTATTGCAATACCATGCTTTTCAACTAAGTCTCTAAATTGTTGGTCAGTCACTGAAAAATTATAACCGTCAATTGATTCTAATTTATCAAGTGTTCCTCCTGTATGTCCAAGTCCTCTTCCTGACATCTTTGCAACAGTTAAACCTAAGGCTGCTAAAATTGGGCATAATGCAATACTTACTTTATCTCCAACTCCTCCTGTTGAGTGTTTATCAACGATTATTTTATTTAAATAACTTCAATCAAGTACTTCGCCTGAATGCATCATAGCGTCGGTTAATTGCGCTGTTTCAGCATCGCTTAAACCATTTAATCTAATTGCCATTAATAAAGCAGCAGCTTGGTAGTCAGGAACATTTTCTGCTACATATTCTTTAATGAAAAAATCAATTTCTTCCTTGGTAAGAGTTTGATTTTCTACTTTTTTGTTAATAATATCAATAATTCTCATAATTATTTCTTTCTATTTATTCAAAGTCTTCTGATATTGATAATGCTAATTCCATCATTTGATTGAATGAATTTTGTCTTTCTTCAGCGGTTGTATATTCATGTGTTATTAAATTATCAGAAATAGTTAAAATAGTTGCAGCTTTTTTGTGCAATTTTTCTGCTACTGTAAATAACCCATATGCTTCCATTTCAACAGCAACTGCTCCGCCTGAATTTTTAACTCTATCTTCAACAGAAAATACTGAATAAAAGGCATCTTCCGATAAAATTCTTCCTTCTTCAAGTTTTAAATTCAATTCCTTAGCATGTTTTTTAATTAAATCATTTAATTCGCTATTTGGAAGCGCAACATTACTTTCATCATTGACTAATAAATTTTTACGGAATGAAGTGCTATCACTATATGCTGAAGTTGCCAAAACTAATGAATAATTTTTTAAATCAGCCTTGAATGAGCCACCTGATCCAATTCTAATTATTGCTTCAACTCCATATTGGCTAAATAATTCATATGAATAAATTCCAATTGAAGGAACGCCCATGCCTGAAGCACAAATAGAAATTTTTCTTCCTTTATAAAAGCCAGTATACATTAAAACATTTCTAACTGTTGACACTAACTTAGCGTTTTCTAAATAGTGTTCAGCAATATACTTTGCTCTTAAAGGATCACCTGGCATTAAAACTAATTTTGCAAAATCATCATCTTTTGCATTTATATGAGGTGTCATATTTTCTCCTTATTCTATTGATAGTTTTTTATACATTATTATTTTATAATAATTAAATAATTAATAAACATTAAATGTCTAAAACGCTAATAAGTCAATACATAAAAAAGCAAAAAGGTTAACTTCTTGCTTTTTTATGACACTATAAACCGCTTTCTGTACTATTTTAATAGCGCCAACTATTTATCTAAGCCAATAGGCTTCCGGGTTCTTTGTTCGGTTCCTTGTTCCCAGTTCCCCTACCAAAATTTGGGTTTCTAGCTCATGGAGTTTACATCGTTGCATCTTAACTAAATAAGCTCGTCTCTGTTGCACTAGTCGTCAACTAAATTGTTGCCTAGACTTATTGGGTCTAGCATGAACACTACTCGCATCACAGCGAGTGCTAGAGCGGATTTTCCTCTACTTTGTAGTAGCAGTTGGCCATGTAATTTAATTTTACCTTAAAACACAAAAAATATCAATGTTTTTTTAGTAAAAAAATATAATTTTAATATATGGAATTTGAGCTAAGTGGAATTGTAATTAATAAACAAAATGTTAATGAATTTGATGAAATAATATCATTACTAACAGATAAAGGCATTCAAAGAATTTTTTGCCCCGGGGTAAATAAAACTAATTCAAAAAATGGCCCCTCAATTTTAATTTTAAACTTAGTTAATTGTGAAGTCATTAAATCAGAAACAAGAAATTTATTACCTAGACTAAAAACTGCTTCGTTAATAAAAAATTTCCCCACAGATTATAGACTTTTAAATATTGCAAATGATTTAAAAACCTTCTTTAATTGTCTTGACAATTGCAATAATATTAATCTTATTAAAGAATATAAACATTGCTTAGATTTTATATTGTCAAAATCTGATAAAGTTGAAGCATATTTATTGTTTTTAGTTGTTTCTAGTTTAGGATTTAAGCCTAACTTAAATTCTTGTATTGAATGTAACAATGTTGAAAATATTGTAGATTTTAAACTATACAAGGGGGGATTTTTGTGTTCGATGCATTCAAACATTGTAAATGCTGATGTATCGTTATTGAGGTCTTGATATTGAATAAATAAAAAATTTGAACAATTTGATTTAAATTGTTCAAATCTAAATGCTATTAAAATCAATAATGAACTTAAAATGTTTTTGTATGATAATAACATTATTTATCGTTAATAGATATTTTTTTTATCTCATTAAACTTAAGTTGCAATGTTTTAAAATCAATGTTATTTGTTTTATTAGAACGTAACATGTCTTCAAAATCATTTAATTTTATTAAATCTTCCTTTGATATATTCTTTTTATGTAAATTAATAATTAAATTAAATGCTTCATTATAAAATAGTAAGTATTGCTTAAAATTATTTTCTAAATATTCAAATAGATTTTTAAAATCATCAGAAATTTTAGCCAATGTTTCTTTGTCTTGTTTATCTTCAATATATTGACTAAAAATATGTTCATTTTTATTCACTATTTTTTTCTTAAAATCATATTGAGCAACTCTTTTTTCAAAATAATCTTTTAAGTTTCAAAATACACCATAATTAGGCAATGAATGAATTAAATTTAATGAACATAAATATTGCTTATAAATTTCTTTACGAATTATTATTAATTGACTGTTTGAATTAGTTTTTTTATGATTATTAACAAGAACATAAGTAATACCTAGTAAAAAAGGCAATCCAATAACTGCACTGATTCCTGAAATAATGCCTATTTTTGATCTTCTAGTTAAAAATCTATGCGGTTTTTTATTTTGAACTTTTTCTTGATTTTTTGATAACTTCATAACAAAATTATATAGTTTAAATTAGCATTGTTATTTTAAAAATAACAGAAAAATTTATTATTTTTTAGTAGATTTTGCCAATAATATAACATTTTCATCAAGGTTTGGATCTTCTTCAATACTTGCAGTAGTATGATTTGTAAACAAAATTGTAGGGATAATAACAACGTCTCTACGTTTATTTTTATAAAAAAATGGGGCTAATCTATCAATTATTAGTTGCTTTAATTGGGGTACGGTTCAATCATTTGAATTTTTAATTGTATATAATATAGCGCCATGAACCAATCTCTTTGCTTCTTCAATTAAATGCAATGAATTTTTTACATAAAATGCGCCACGAGAAACTATTCTTGGTTTACCAATAATTAGATTTTTTTCTTTATTAATTGCTAAAACAACATGCATAAATCCATTTTGGCCTAATTCACTACGTTCTTTAATAATTTGAGAGTTAGTTTTTGATAAAATATTGCCATCAATAAAAACAGGCCCAAAATAAACCTTTTCATTCGACAAACGAACTTGATGATTTTCTAAATAATAAACATCCCCTAATCTAGCAATCAAAATATTTTTAGCATCAACACCGCTTTCAATAGCAGTATAGCCATGAACAACGCTCATTCTATATTCACCGTGATAAGGCATAAAATATTTTGGTCTAGTCAATTCAAAAATTTTTCTATGTTCATCTTTGTAGGCGTGGCCCGAGGTATGTAAATAACCGTCTACTCCATTTTCACGAATTATTGCGCCTAATTTATATAGTCTATTAACTAATAATTCAATTTTAGATCTATTTCCAGGAATTGGACTAGATGAAAAAATAATAGTGTCTTTTGGTTTTATTGTAATATTTGGATGTTTTCCTCATGACATCTTTGCAAGTCCGGCTAGTTCTTCACCTTGAGAACCAGTTGTTAAAATTAATAACTCATTATCTCCAAATTTGCTTAATGATTTTTTGTCAATAAACACTTCATCTGGAACATTAATATAACCTAGTCTTCTACCAATATCTATTCCATCAACCATAGAACGGCCGAAGGCAACAACCTTTTTATTTAAAGAAGCGCCTAATTCAATAATTGCTTTAATTCTAGTTAAATTAGAAGCAAATGCAGTTACAATGATTTTCTTTTCTGCTTCCAACATATATTTTTTAATATCTTTTAAAATATCTGACTCTGATGGTGAATGATTAGGACGCATAGCATTTGTTGAATCAGAAAATAAAACATCAAGGCCTTCGTCGCCAATTTGTTTAAGTTTATTAAAATCTGTAAAATTTCCAATAGGTGTGTAGTCAAACCTAAAATCACCTGTCATCATTAATGAACCATTAGGGGTTTTAATTCTAATGCCAAAAGCATCAGGAATTGAATGTTGGGCAGTTCAAAAATCTACTTGACAAGGGCCAAAAGTTCAAACCGCATCTTTTTCAGTTTCAATAAATTCAATATTACGTTTTATCTTCATATCCTCAAATTTTAGTTTTAGATATTGAATAGCAATTCTAGGTGCAAATATTTTTTTAACATCTACTTGTTGAACTAAATATGGAACACCTCCAATATGATCTTCATGTCCGTGGGTTATGAAAAGACCTTCGATTTTATCTTGATTTTCTGCTAAAAAAGTGAAATCTGGAATAGAACCATTTACACCTGTTACATATGAATCGCAAAATTTAATTCCGGTGTCAATAATAAAAATATGATTTTCATATTGAATAACCAAAGTACTTTTACCAATTTCTTGCATTCCGCCTAATCCAAAAATTTTAGTTGGGCTCATAAATCCTCCAATTTTTATAATTATTATAATGAATAAATATTCAGCTTTTTATTGTTAATATTTTAGCAAAAAAATACGAAATTAAAAAAAATACAAATTTTTTATTAAAATTTTAGATATGAATGATAGTAGGGTACAAAAAACAAAGATTCCTAAAGTATTAGCAAGTTTTAGTTTAATTTTAGAACTTATTGCATTAGCATTACTTATTTATGTTATATATAGTTTCTCTTCAATTTGAAAAGAAACACTTGCAGATATAAAGGGAATTATAAAAAATAATAAAGACAGTTTTGAAACATTTTGAAAGGAATTTTCTAAATATTGAATAAACTTTTATAAAAATAATAAAGTTTTAATTAGTGCAATTTTAGCATTGCCAATATTGTTATATGTTTGCTTTATATTTAGCATGTTAGTAATAGCAAAAGCTAGTTGATCATTGCACAAAGTTCCAATAATACTTATTATTGTTACATTATGTTTGCCAATTCTATTATTATGTGAAATAGTTAGAATAATATCACTATTTTGAATAATAATCAAAAAGCAAAAAAATTTCAATAACCAAGTGTACTATGGACCACAAGTTGCTAACATACAAAATTAATAAAAAATTCGTAATTATTTCTATTACGAATTTTTATTTATAAAATAAGAAAACAAAAAGAAATCTTATTTATATAATTTATCGATTTTATTGCTTATTGCTCTTGCAGCAATTGTCCCATCAGCTGTTGCTGTTGTAATTTGTCTTACATCTTTTGCAATAACATCGCCTATTGCAAAAATATTAGGAATTTTAGTTTCCATATTTTCATTAACTTCAATAAATCCACGGCTATTTAATATTTCATCGTATTTGCCTTTTATAAATGCTGTGTTAGGTTTAAAACCAACATAAGGAAAAATGCAACTTATATTCATTTTTGTTTCTTTTCCATTTACATTTGCAATAATTTCTTCAACTTTTTCATTGCCCAAAATTTCTTTAATAGTTGAATTTTCATGAATATAAATATTACTTTTAGACATTGCTTCATTGACAAGTTTTTTTTCTGCAATAATTCCATCACGAACAAAAATATGAACCTCAGAAGCAATTGATGCTAAATAAGATGATTCTTCGAATGCCGAATTACCACCACCAATGATTGCACAAGGTTTATTTTTATATAAAGGGCCATCGCAAATTACGCAATATGATACGCCTTTTCCTCTATATTCTTCAATGCCTTTTACGTCCAATGGAACAATTTCTTTCATGCCGGTTGCAATAATTATTGCTTTTGATTTTATTGACATGCCATTTTCCAAAATGACTTCTTTTTCAATATCTGATATAGAATTAATTTCCTTTACATTACCGTAAATAAATTTTGAGCCATTATCTCTTGCTTGTTTAAGCATAGCTTTAGAAAGTTCAAAACCACTTATAAAACTAAAACCTGGATAATTCTCAATTTTAGATTGAGAAGAAACTTTTCCGCCTGGTACTCCGCCTTCAATAAAAGCAACATTTATGTCATTTCTTGCTAGATATATTGCAGCCGTTAATCCTGCTGGCCCTGCCCCAATTATTAAAACATCATATAAGTTATTATTTTCCATATATTTCTCCATTACTAAAAAAGCAGCAAATTATTAAGCTGCTGTTTTAGTTTCTATTTGATTTTTAGTAGTCGATTTTTTAAATGCAAATGAGTTTGAATTTTTAGGTTCATAAACTATAAAGTAAAAGAATTTTGAAAGCTTTTCGTTATTATATGTATTAACTTTATAAACATTGTAATTCAATTTTATAACGAATTCAAACATTACAGTCAATGCCAAACCAACTATTATATATAAAGCAGAAATTACTTTATAAATAGTATAACTGCCATCACGTAAAGGTTCCATTCCAAAACGAATTATTCCATAATAAACAATATATAAACCACCAGTTGTTCCTGGACGTAATCAATTTCAATTATTTAATATTCAAACTAAGATAATATAAGCAGCTAAGTTTGCAATTGATTCATAGAAGAATAAAGGATAACGGTATTTTCCATCAATAAACATGTGATCTCTAATTAATTTTGGCAATATTTCAAATGCGACAGAGCCCTTTTCCATAACGCCGCCATAAACTTCATGGTTTGCGAAGTTTCCCCAACGTCCAATTGCTTGTCCAATTAATACCGCTGGTAAAATTATAGTAATTGCTTTTTTCAAATCAATTCTATTTTTATTTTTTGAAAATAGAATATAAAGAATACAACAAATTGTAGCAGTTATGACACCACCATGTATTGATAGTCCACCCTTTCAAATAAAGAATGCATTATACCAAGGACCATCTGCCCAACCTTTATGTTCAATTAGTTGTTGAATAACGAATACAAATCTAGCTCCGATAATAGCAGTAGGAAGTGCTATAAATACTAAAATTGTTAGTTGTTCGAAAGTATATTTTTCTCTTCTTCAGAAAAAATAAACAGTCAATATAGCAGTGAGCATTCCTAACATCATTGTTAAGGAATAAACATTCATTGGGCCTAATTTTAATTGAATTCCTGCTCAATCTTTAACTTGCATTTTCCCTCCTTATAAATTTGCCACTTACATTAAAATATAATTATAAATTATACAAATCAAAAAAATTTTTTTTAATAATCACTATAAAAGTTTTGCTAAATATTGCCCCGTGTACGAATTTTCACACTTTGAGACCTGTTCAGGTGTTCCTGAGCAAATAACTTTTCCCCCTTCGTCCCCACCATCAGGGCCTAAATCAATAATATGATCAGCACATTTAATTAAATCCAAATTATGTTCAATTACAATTACTGTATCGCCATTGTCCACTATTCTATTTAATACTATTAATAAATTAGATATATCGTAAGGATGCAATCCGGTTGTTGGTTCATCTAATACATATAAAGATTTGCCTGTTGGCTTTTTTTGTAGGTATGTTGCTAATTTTACCCTTTGAGCTTCACCACCGCTTAATGTTGTAGCATTTTGGCCTAATTTAATATAGCCAAGTCCAACATCTAATAAAACTTGCAATTTTTCTTTAATTTGGCTCTTGTTTATAAATAATGCATAGGCTTTTTCGATTGTCATATCCAAAACATCGCCAATACTTTTACCATGATATTTTATTTCTAGCGTTTCTTGATTATAACGTTTCCCGTTGCAATGGTCACATGTCACATAAACATCGGGCAAAAAATGCATAGCAATTTTGATTACACCATCACCTTCACATTTATCACAACGCCCACCTGGAACATTAAATGAAAATCTTGATTTTGTATATCCTCTGATTTTACTTTCTTCGGTATTTGCAAAAATTTCTCTAATATCATCAAAAACACCGGTATATGTTGCAGGATTTGATCTAGGAGTACGCCCGATTGGATTTTGGTCAATTTGAATTAATTTATCAATGTTAGTTTGGCCAATTAGTTTATTATCTCTTTGAATATCATAATATTGATCTAATAGATATTTATTAAGACTCCCGACTAGTTCATCGTTTATTAACGTAGATTTTCCAGAACCGCTAACTCCAGTAATTGCAATAAATTTTCCTAATGGAAAAGTGACTTTAATATTTTTTAAATTATTTAATTTTGCATTTTTTAATGTTAATGTCTTGCCATTTCCACTCCGCCTAAATTTTGGCACCTCTATTTGTCTTTCTTTTGACAAATATTGGCCAGTTAGACTTTGCTTACATGCAATTATATTTTCAACATTTCCACATGCAACAATATTTCCACCATGTTCGCCTGCTTGAATACCAATATCAACTATATAATCTGCTTGTCTAATTGTTTCTTCATCATGTTCAACAACAATTAAACTATTGCCTAATTCAACCATTTTTCTTAAAGAGTTTAGCAATTTTTCATTATCTTTTTGATGTAAGCCAATTGATGGTTCATCTAAAACATATAAAATGCCTGTTAGATTCGAGCCAATTTGTGTTGCTAATCTAATTCTTTGAGCTTCACCACCGCTTAATGTCTCAGCTTTTCTATTCAATGTTAAATACTTTAAGCCAACATCAATTAAAAATTTAATTCTATGTTTCAATTCAGACAAAATTAAAGAGGTAATTTCTTTTTCTAAATCACTTAACTGCAATCTTTCTAAAATAATTGCCAATTCTCCAAGCGACTTATTGCAAATTTCGTATATATCTAGATCTAGAATTTTGCAAGACAATGCATACTTATTCAAACGCTTTCCATGGCATGAGGGACAAGTAATGTCTCCAATGTATTTACTATAATAAGCCCTAGCTTCTAGTGATTTTGTATCAAAATACTTCCTTTGTAATTTATCAACAATACCTTCAATAACTTGAAACTTCTCGCGTTTTGTGCCACTTTCAGATAATAACACATATGATATTTCTTCTTGCGACCCATGTTGAATAATATCTAGTTCAAAAGAAGATAAAGAATCAATAGGTTGATCTTTATCAATATGATAATAATTTAATAGCTTGTCAAATTCTTGCCATTCTAAATTGTAAGTATTCATGAAATTTCTATAATACTTTATTGCTCCTTCGTTTATAGAAAGCTGCCTATCGGGACAAATTAAATCAAAGCTTGCCTTTTGAATAACACCAAGTCCCTTGCATTCAGGACACATTCCGGCTGGAGAATTAAACGAGAATAATTTGGGTTCAATTTTTGGCATATCAAAGTCGCCAAAACTGCAACTATGATGTATTGAATAGGTATTCTTTTTGCTTTCATTAACAATAGTTGAAATTAAACCATTAGATTGCTCTAATGCAACCTCAATTGCACTTGATATTCTTGATTTTATATCATCATCATTTGATAATTTAACTCTATCAATAAACAGATCAATATTTCAACGTTTATTTTTATCTAGATTAATTGAATCAACATTTTCTAAGAAATAAATTTCATCATTTATTAAAACTTTTATAAAACCATCTCTTTTTAATTTATTAAGTAATACTTGGTGGCTTCCTTTTTGATTATTTACTAATGGGGCTAATATGTATATTGTTGATCCTAATGGGTTTTTATAAATACTAGAAATAATGTCTTTTGTTTTTTGAGCAGTAATTTCAATTTTGTGATTTGGGCAATAAGGTTTTGCTACTCTTGCATATAGCAATCTTAAATAATCATAAATTTCAGTAACAGTACCAACTATACTTCTGGGATTAACATGTACTGTTTTTTGTTCAATAGAAATTGCTGGGCTCAATCCTTCAATGCTATCAACATTAGGTTTTTTAGTACCACCTAAAAATTGTTTTGCATAATTAGAAAGGCTATCAACATACCTACGTCTGCCTTCTTCATAGATTGTATTAAATGCTAAAGAACTTTTACCAGAGCCACTGACACCAGTAAAAACCACAAATTTGTTACGAGGAATATCTAAATTAATATTCTTTAAATTATTCTCTCTAGCACCTTTTATTATTATTTTATCGTTCATTTTCTTTGATATAACCCTTTTCTATTAAATATTGTTCTGCTTTCTTTATCCTTTTTTTAAAAAATTTATTAATGAATTTTAAGTATTTCATATCCATTTTAATCTTATTAGGTTGCTTAAATTTAAATTTTTTAAGTTGTTTGATTCAATCTAAATGTCTTTTTCTAATAAAAATTTTTAGTTGTTGATTAAAACTTTCAAAAAACCTAGTTTTGCGATTCTCATAGTCATTTAAAAATGCAGTTTTTGCAATTGATTTTAAATAAATAGGATAATCAAATATTAAACTATTGTTGAAATCAAATATTGGAGCTGGCTTTGTTAAAATTCCACTATTATTATCCAACATCATGCCAAAATTATTTAGATGCCTATCCCCATTGCAAATTAAAGCATCAAAAACCATTAAATCTTCAAATAACTCTTTGCCATAATAAAAAGCAATCTGACTACATAGTTTTAAATTATTATCAATACTATTGGGATCTATGATTTTAGAAAATGGGATGAATGAAAAATCTTTGTTTGTAAACAATTTGCAATCACTAACAAAATGCTTTCCATACTTAGTAATTTTATATTCAACTGCCGGTAAATTCAAAAATTTGGCAACTTGACTTGCAAAATATTCACTATAAACATCGTATAAGTTTTTATTTGCAAATTTTGAGTTTCGTTTATATAAATGAATTTCGCCATCCACCTCAACTCATGCTTTTAACATGTCGCCATCAATAAATCAACTAGGGCTTTTAATTTGTAGTCTGTTAGTTTCTGTTAATTTTGCTTTATAATCTAAAAAATAATTGCAAGAATCATTATTGAAATGATCAAAATAGTTTACATCTTCTCAATTTAATTTGCAACTTTCTTCTAGGCTACATATTCAAAATGAATTGCACAAATTCAAATAATTATTATTTAAAATTAATTTCTTATAATTTAAAAAGTTTTCAATTAATCCTTTGTAATTAGGCCTTTGCATTGGAATTAATGAAATATTTAAAATGAATTCAAGTCATTGTCTTGGCGAATATTCATTATATTTTTGCTTTATAAAAATAAAATCATCAAGCAATTTAACATTGCTCAATGTTGTTAATTTTTCATCTGCTTCAAAACTTAAACAAATCCTGTTTTTACATTTTAAATAATATGTTTTCATTACTAACTAATTTATTTCGCCATCAAAATCGTTCTTATTATCTTTTGATTTTGTTTTTGGATCTTCTTTGTGGTCTTCCTTTGCTTGATTTTTATACTTTTCTTCAACTTCTGCAATTTCTTTTGAAAGATCACGGTGCAATATTTTTTGTAAATTTTCTAGCATGCTTTTTTCTCATGAACCGTCATTAGGAATATTATTTATTCCCATTCCTCTTAATTCATCGAATGCGCCCTTGAAATAATCAACCATTTCATCATCACGAACATTTCCAATTGCTTTGGCAGCTTTATACATACTATCAACTAATTTTTCCATACTTAGATCTTCATAATTCATTTCAAATGGAGAACCAGACATAAATTTATTATCAGCAATTTGTTCTTTTAATTTTTTTGCTTGTTGTTTTAAATCATCAAAACCTTCTAAGAAATTTACAAATGAATTTTTAAAATCATCCATACTACTCATATCTATTTCTTCTTCATTTGAATCGCCATCATCTGATTTTTCATCAGATTCTTCGTCTTCTGATAATTCAGATTCAAGAGAAAATTCCTTAGTTCCAAACCCACCATCATAATCTTCTATTGACATTCTACGTTCAATAGGGAACGCTTCTTCTATTGCTCAAATTTTAGTTCCCAATAAATTCTCTACTGAATTTCATGCATCAATACCATTTTCTATTTTTGTTAATTTAAAATCAAAACTAGATGGTTCGGTTTGCAATAAATTAAGTTTTTCTGATAATTCAAATAATTCGTCTCATTTACTAAAATCGCTTCTAGTTACGTTGTTAATATCATTTGAAGGATTTGTATGAAAATTTTTAAATTCATTTTTCTTTAATTCTTTTAAATATTTTAAAGTTTCTTTTCTTTCGGAAATTACACTTTGACTAATTAATTCAGGATCGATTTGAAAATCTTCATCAAATTCTCTTTTATCGTCATAAACAAAATCAATTTTTAATGCCAATGAAATAATTTCTTTTGTAGGTAAAATTGCAGAGAAAATAATTTCATTTTGTTCAAGTCCATATTTTTTAAAGTCAAAAGAATTTTTATTTTCTTCATCTTCATTTATTAAAAAATCATTAAAAATATTTAGGGAATTAAAAGTGTTTAAATCTGACAAAAATTCTTGTGATGCTTGTAAATCACCAAATACAAATTCAAACGGAACAATTGTTTTTAAATAATTTGGAATTGTATATCAATGAAATACAACACTCTTTTCAAAGCCAAAAAATGCGCCTAAAACATCCACTAATTCAGAAAAAGAAATCCCTGAAGTTATTGGAAATGATATTGGTTCAAAATTAATTTTTTTAACTGCTTCTATTGGCTTAAATTCGTAGTCAAAAGCTACATTTAATTCTCTAACTGTTAATTTTAGATTTATTCCTAATTGCTTCTTAATCATTTTCTACTCCTCTATTGTTTTCAAATTCATGCAACATATATTTTTTAGTTATATTTTTTAATCTATATATGTAATCAAGTATTGCTTCTCCCAAACCATTTTGTTCAACAGCACTTGTATGAAAGTGGCTATGTTCATGTACGGTTTGTGTTGCATTTGCCATTGCATATGAATATTTTGATATTGACAGCATTGGTATATCATTATTTCCATCCCCGATTGTCATTATTTCATCTTTAGATGTTTCTACATTCTTATAAACATTTTCTAACATTCAAAGAACAGCAGACCCTTTTGAAATATTTAATGGTGATATTTCAATATTGCAATTTGTTGGAATCATTGCCAAATTTGTTATTGACTTAAATTTTTCAAACATTTGCAATGCTGACTCTTCCGTTTCAGTTCCATCTGCTGAATAAATTTCAATCTTGGTTGGTTCAATAAATTCACCTTTATATAATTTAGGAATCGAGTGTAATAATTCCTTGCTTATAAAGTGATATGAGCAAATATATTCAATTGATTTTTGATTATCTTTTGTATAATAGTAATTATTATTGTCTCATAATAGAACTTGAAAATCATTCTTTTTAGCAATGTCAAGAATCAATTTTGTTGTATCAAAATCTATTTTTCAACAGGCAATATATTCTCTTGATTGCATATCAAAAATTTGAGCGCCGGAAGATCCTATTAAATATCTTGCTTTTAATGTTTCGGCTAGTTTTAACATTCTTTCTTGAATTGGATTTCCGGTGCATATATTAAAATCTAATCCATTGTCTACTGCAAAATGGATATCATCAATAGTTTCTTGCTTTAAATTAAAATTTTTATATATAGTACCGTCAACATCTGAAAAAGCAATTGGCTTCTTGTTGTTAGTAACTTTTAAAATTGTTTGACTCATAATTCTCCTTGTTTTTTTATGCTCTTGCCTTTGATTTATGCTTCTTTTTTAATACCATTATAATAATATAAATTATAATGCCAGAAATGGCAACTGGTGGCACAATATAGCTTAATAGTCTAAATGTATAATGCATTGTAGTTTCGGGTTCTTCTGATTTTAGAAATTCCGTATAATCTAATAAAAAATGATTTATGGCATATGTTTTTAAATTGAAATAATTCTTTACATTTAAATCCGAGGCATCTTTTTTTATTTCATTGCTTAATTGTTGTTGCATTTTTTTTGATACTAATTCAAACACAAAAAGTATTTTTAAAAGTTCGTCATTTGTTCTTAATCTTATTTGGAAATATTTATTAAAAACTCTATAAAGATTAGGATCAGGATCATTTTTTAATAAATTAATAAAACTATTTTGATTCTTTATATCAAAGAAGGGATATTTAAGATCATTATCCAACTCCAAAGCTCTATTGTATTCAGACTTTTCTTTAAAATTCCAATCTTTTACTCTATTGTTATAAAAATCTTCAAATCTTATTTTTTTAAATGGATTTTTAGGGTCTTCTATATTTAGATTTTGTAAGAATAATTTTTGATTCATTTCATCAAATGAAAATCTGTGTTCTTTAAATATTTCAACTATTCTTTTGGCAACACTTAAATCTTGTTTTTCAATTCCATCATCATTGCCTAATTCTAAATTATCTTGATTATCTTTTGATTTTTTGCATGACAGTGAAACTAATGGGATAGTTAGTAAAGTTGGAATAGTCAATGATAAACCCAAGAATTTTTTTATTTGTTTTTTGTTCATAATATTTATTTATTATATTTTACTAAAATTCCAAACTTAAAAGATTTAAAAGGGAAATAAATGCTAAAAACTATACACTAGAAGTTTTTAAAAAATAACAAATTAAATTAAATTTCAAACTTTATAATTCCTATTCTATTATTCTAGATACTTAAATCCAAAAAGTTTTCAACAAAAATTAACCTTTTAGTTCAAAAATATAGTTTTTTTTATTTTTTTCAATATATCGAATATAAACCGTTTAAAAAAACAATAAAATATTCCCCGGAGATTATTAAGTCTCTTTTTTATATTTACTAAATTTAAAAAAGGATAAAAAATTAGAAATGAAAAAATCAAAGAAAATATTTATAACATTATGTGGAATAGCAACTACCGCTATTTTGCCAGTTGCTACTATTTCATGTAATGATGATAAACTAGCAGAGAAAAATGGAAAAGAAAAAGCAGATGCTGCTTTAAAACAAGCAAATGCATTAGCAGAAGAATTGAAAAAAAATCCTGATTATTCAAAAATTTTAGAAACATTAAACAAAGAAATTGCCGAAGCAACCAAATCATTTAAAGAAGCAGGTTCATACGGCGATTATCCAGCAATAATTTCTAAACTTTCAGCAGCAGTAGAAAATGCTAAGAATGAACAACAAAAAGTTGATCAAGCAAATAAAAAAATTGCTGATGAAAATCAAAAAATTAAAGAAGGCGCTAAAGAATTATTAAAATTAAGCGAAAAAATTCAAAGTTTTGCTGATACAATTGCATTAACAATTACTAAATTAGAAGGAAAAAAATTCCAAATTGATGAAACATTTAAAAAACAATTGATTTCAACAATTGAATTATTAAATAAAAAATCAGTCGAAGTTAAAACTTTTGCAACAGTAAATACCATTAAAAAAGATTTCCTTTTATCAGAACTAGAATCATTTAAAGAATTTAACACCTCATGACTTGAAAAAATCGTATCAGAATGAGAAGAAGTTAAAAAAGCTTGATCAAAAGAATTAGCTGAAATTAAAGCTGACGATGATAAAAAATTAGCAGAAGAAAATCAAAAAATTCAAAATGGTATCGCAGAATTATCAAAATTAAGCAAGGAAAATTCAGATTTAGCAAAAACTATTGAAGAAACTATTGCTAAATTAGAAAAGAAATTCCAAATTCCAAAAGATTTTAAAGAAAAATTAACCTCAACAATCAAATTATTAAATAAAAAAGCTAACGAAATCAATACATTTGTTTCTACTGTTTCTAAAAAGACAGAATTTGTTCTTGAAGAATTAGAATCATTTAAAGAATTAAACACTCTTCAATTTAATGAAATTAAAACTGAATGAGCAAAAGTACAAGAAGCATGAAAAAATGAATTAGCAGAAATTAATGGTGTTGAAGAATTAAAGAAATTAAGTCATGAAATTTTTGAATTTTCTAATTCAGTAAAGAAAACTATTTCTGAATTAGAAAAGAAATTTAAAATAGATGATCAAAATAATAATGAAGAAGTTAAAAAATTCAAAAACGAATTAGAAAATTTTGCAGATCAATTATTGCACAAGAGTCGCCAAATTAATGAATTTGCTACAGTTACATCAGCACAAGAAGATTTTTCTTTATCTGAACTAGAATCATTTAAATCATTGAATACCACATGATTCAATGAAATGAAGTCTGAATGAGCAAGAGTACAAGATGCTTGAAAAGATCAATTAAAAGAAATCTCAGCAAAATAATATAAATTAAAAATACAAAAAATAGTGCTACTAATGGGCACTATTTTTAATTGTCAAAATTGAAAATTGATCTTTTATTAAAAGCAAACATTTATCTAAAAGTTATAATGTTTTTTAACAATATCAAAATCTAATTTTTCAAAAAATCATAGCAGCAATGGTATGGTTTAATTGTTGTACATTTTTTAGGTCAAATGAAATTAAATTAATATTTTTATTAATTATGCAGTCATATGAATAAACAAAAAATAAAGGATTTATTTTATAACTAGATATTTCAATGTTCCCCCCTTTTTGCTTTCTAACTTACAAATTATCCAAAGATAATGTGGTTATATATTATTTATTATTGAAGCATTTTTGGTTTTCAATATTTTTTAAAAAATAAGATAAATAAATGATTAAAAAAATTAATAAAAAAATGGGGCGTTTGATGGGGATCGAACCCACGTATGCCGGAGCCACAACCCGGAGCGTTAAACCACTTCGCCACAAACGCCATTTTGTTAAATTGCTTATATATTTTAACATAAAATACAAATATTATTGTAATTTAGTGCCAAAAAAAACTTAAACTCTTACTTTATATTTATTTTAATAACCCTTATAAATTGAACAAAAAAACAAGATAATTACTTTTGGGTAGCTATCTTGTTTTTTAAATTTATTTATTTGTAAAATACAATGGTTTAATGTTTGAAATGGCTTTCTGTCAAAGATTGAGCCGTTGCTAGTTCATTTTCAATTTCTTTGATTTTCTCTTTCATTAATTTATCATTACCAAGGTCTTGATTTAAATTAATTTGTCCCAAGGCATTTATTGCTTCATCAGCATCTTTAATTCCTCTTTTAAAGACTTCTCTTTGATCATGTTTATCAGAAATTAAATAGTTAAATTGTTTCCAATATGTATAAAATAATTTAATTAATTCATTTAAATTGTTTATTTTGCTTGTTTCTACTTTTAGGGCATCCAAATCTTCTTTTTGTAATGCTGAATTATATCCTTCTTCAATTAATTTTAATGCATTATTGATTCATGATTGAAGTTTTTTGAATCCATCAAGTTCTTTAGCTGATTCAGGAACTAGATTTTCAATTAATTTTTTAGTATCTTTTGCTTCATTTTGAAGGTTACTAAATTCCGTTGTTGGATCTTCTTCAACAATCTTTTTTAATTTGTTTTCCAATTCAGTTATTTCAGAATCACTAGGTATTGTTTCTTTGGCTAATATTTTTTCAAATTCTTTTGCAAGTTTTTTTTGTAAAGCCGGCGACAAATTAGTGTCGTTTTTATATTGGCTAAACAATTGACAAAGTTTCCAAAGATTATCTGAATCTTCATCTGCTTTCTTGAGATCTGATAATTTTTTTGTCCAAGATGGATTATTGAAAATAGGTTTTTCTAATACTCTTTTAAATTTATCAAGGAAAACGCTATAAAATTCATTGGCTTTAATTTGTTTAAGATTTTCAATACGGAATTTAATTCTATGGGCAATAGGCTTTTTCTCTTCTTCCATAACCTTTATTCAAGATTCAAGTTTTCCAACAAATTCATCTATTTTTTGAATTTTTTGGTTTATATCAGTAATTTTTGAATTTTCAGAAAGGTCTCTCGCTTCTTGCGATAATTTTCTAGCTTTTTCAAGCTTATTTTTTATTTCATCCGCATTTAAATCATCAGGTCTTTTGCTAATAACCATAAGATCGTCTAAATTTTCAGTTTTTAATAACGTATCTAATTTTTCATTCTTTTCTTTCAAACTAGCCATCAATTCTTGTTTTTTAGCCTTGATTTTTTGGCCTAATTTAGATTTTGCTTCATCAAGTGCTTTAGTTGCATTTGTAATTTCGTCAATAGATGAATTTTTATTAATCTTGTTATTATCTTCTAACAACTTTTGGGCATCATTTGTTCCAACTTCTTTTGCATCTTCATCTTTAATTAAATCTTCTAAAGCTTTTTTTGCTTCGTTGAATTTTTGCAATTTTTCAGTTTTTTCTGCTTCTAATTTTTCTATAGCTTGTTTTAATGCTTCGTTTGCATCTTTAATTTTTTGAGTTGCATCTTTCAATGATTTTATTGTTGAAACATTAGTTAAGTTTAATTTATTAATTTCATCCAATGTTTTTTGAGCAGCAGATATTGCATCTGCTGGTGCTTGTTTATTATTAGATGCAATTAAATTACTTAATTCTGTTTTTGCGTTAGTTAAATTATTAAATTCAGCTTCTTTTTTAGTCTTGATTTCTTGATCTAATTTAGATTTTGCATCTTCAAGTGCTTTAGTTGCCTTTGTAATTTCATCAAGGGTAGAGTTTTCGTTAGCATTGTTGTCTTCTAAAACTTTTTTAGCTGCTTCTACTCCAGTTTGAATTGCATCTTCTTCTTTAACTAAGTTTTCTAAGGCAGTTTTTGCTTCGTTGAATTTTTGTAATTTTTCGGTTTTTTCTGCTTTTAATTTTTCAATTTCTTTAGTTAGTTGTGTTTCTGCGTCCTTAATTTTTTGAGTTGCATCTTTCAATGATTTTATTGAGGAATCATTGCTTAGACTTAAACCGTTAATTTCTTCTAATACTTTTTTAGCATTAGATATTGCTTCAGCTGGTGCTTGTTTAGTAATTAAATCACTTAATTCTGTTTTTGCATTAGTTAAATTATTAAATTCAGCTTCTTTTTTAGCCTTGATTTCTTGATCTAATTTAGATTTTGCATCTTTAAGTGCTTTAGTTGCGCTTGTAATTTCTTCAAGAGTTGAATTTTTATTAATCTTATTATTGTCATCCAAGGCTTTTTTAGCTGCTTCTACTCCAGTTTGAATTGCATCTTCTTCTTTAACTAAGTTTTCTAAGGCATTTTTTGCTTCGTTGAATTTTTGCAATTTTTCGGTTTTTTCTGCTTCTAATTTTTCTATAGCTTGTTTTAATGCTTCGTTTGCATCTTTAATTTTTTGAGTTGCATCTTTCATTGATTTTATTGTTGAAACATTAGTTAAGTTTAATTTATTAATTTCATCCAATGTTTTTTGAGCATCAGATATTGCTTCATCTGGCGCTTGATTACTTGAGGAGGTAATTAAATTACTTAATTCTGTTTTTGCGTTAGTTAAATTATTAAATTCAGCTTCTTTTTTAGTCTTGATTTCTTGATCCAATTTAGATTTTGCATCTTCAAGGGCTTTAGTTGCATTTGCTATTTCTTCAAGAGTTGAATTTTTATCAGCTTTGGTTTTAGTTAATGCTGTAGTAGCTTCGGTTGTGTCGACTTCTTTTGCATCTTCATCTTTAATTAATTGTTCTAGCGCTGTTTTGGTTGTATTGTATTTTTCTAATTCTTGTTCTTTTTGTTTTGAAATTTTATCGGTTAATGATTTAAGAGCATTTTGGATTTTTGTTATTTTTTCTTTTATTTGTTTAATAGAATCACTATTAGTAATGTTGATATTGCCGAATATGCTCAATTCATCCTTTTTATCAACATTATTTGCATCTGGTAAGGAAATTAATTTTTCTAATTCAGATTTTTTGTTTTCAAATTCTTTAGTTAATTGTTTTTTTGTATTGTCAATTAATCTTTGTAATTCTTCTTTGACATGAGTTAATGCTTCGGTTGAATGTTGAATTTTTTCAATAGTTGCAGTTTTATCTACATCGTTTTCGGATAAGGTTTTTTGAACATCGGCTATGCCCACTTGAACTGCGTCTTCTTCTTTGATTAAGTTTTCTAATGATGTTTTAATAGCATTATATTTATTAAATTCATCATCCTTTTTATTGTTTATACTTGTAGTCAAAGAATTAATTGCTTCTGTAATTTTTTCTGTTTTGGCTTTTATTTCTTTTATTGGAGTATTTGAATCAACTGAATTGCCATTTAAAACATCAGATTCTTTTTTATTATCAACAATTTTGGCATCACTTGATTCTAGTAATTTCTTTAATTCATCTTTCTTGTCATTGAATGTTTTTGTTGCATTGTCTTTAGCATCAGTAATATTTTGTGTTAATTCAGCCTTAACGTCTTCAAGAGCTTTAGTTGCTTTTGAAATAGCATCAAGAGTTGAATTTTCGTCTGCTACATTAACTTTCAATGCTTCTTCAGCCTTAGAAGTTCCAACTTCTTTTGCATCTTCTTTTGCAATTAATTCTTCTAATTGTTGTTTTACTAGATTAAATTTTTGTAATTCAACAGCCTTATCTCCTTTTGCTTTGGCTATTTTTTCTTTTAAGGTTGATATTGCATTTTCGATGTCGCTTGTTTTTTGTTTAATTTGTTTAATTGTAGAATCATTATCAATTGTAGTTTTATTGTATTTTTCTTCTTCGGCTTTTTTATCAACTTCAGATGTTGTAGAATCTAAAAGGCTTTTTAATTCTTGTTTCTTTGAATTAAATGTTTCTTTAGCTTGTTGTTTTGCACTTTCAATGCTACTTTTTAATTTTGTTTCTTCGTTAGATATTTTTAAAATAGCATTTTTAATTTCTTCAATAGTTAACTTTTCATCAACTTTTGTTTTTAAAGCTTCGTTTGCACTATCAATGCCAACTTCTTTTGCATCTTCTTCTTTAACTAGTTTTTTTAGACTTTCTTTAGCCTTTTCAAATTCTTGAATTTTTTCGTTCTTTTTATCTTGAACTTTTTTATTTAAGGTCTTAATTGCTTCTTCGATAGTTGAAGTTTTTTGTTTAATTTCTTTAATAGAATCTGTTTTAGAAATTGAAGTATTAGTTAAAACATTTAATTCATTGCTTTTATCAACATTATTTGCATCACTTAAGCCAATTAATGTTTTTAATTGTTCTTTTTTAGTATCAAATTCTTGTTTAGCTTTTTCTTGAGCCTTTGAAATTTTTAGTTCCAATGCTGCTTTTGCTTCTTGCAATGCCTTGGTTGCGTTTGTTATTTCTTCAAGAGTTGAATTTTCATCAGCTTTATTATCTTGCAATGCTTTTTTAGCTTCGTTTACTCCAGCTTGAATTGCATCATCTTTTGCAATCAATTCTTCTAATTGTGTCTTTATACTATTAAATTCAGCTAACTTGGCTTTTTTGTCTTTTTCTTGGGCAACATTATTGCTTAGTTTTTCTATGGCGTCCTTTGTTTTTATAACTGCATTTTTTAGTAAATTAATTGGTGAGGAGTTATTTAATTTTTCAACTTCTGTCAATACTTTTTTGGCTTCTTCAATTGCTTTTGCTGACGCATCAGTTGATTTAGCAATTAATTCATTTAATTTGCCTTTTTCAACTGTTAAATTATTAAATTCAGTTTCTTTTTTAGTCTTGATTTCTTGATCTAATTTAGATTTTGCATCTTCAAGTGCTTTAGTTGCCTTTGTTATTTCTTCAAGAGTTGAATTTTCATTTACTTTATTATTATTAAGAGCTTTTGATGCATTTTCTGTTCCAACTTCTTTTGCATCATCAGTTTTAATTAATTGTTCTAAAGCAGCCTTGATATCATTAAATTTTTGCAACTCATTTTTTAATGCCTTAACGTTTGCGATTTTTTTGTTTAAAGATGATATTGCTTTTTTAATTGCTTCAGTCTTTTCTTTAATTTGTTTTATTGTATCTCTTTCAAGAATATAAGTGTTTGCCAAAATACCAATTTCAGTTCTTTTTTCAATTGGTTTTGCATCTTCCGAGCCCACTAAAACTTCTAAATCTTTTTTAACTTTATCAAATTCTTTTATTGAATCTTCTTTGGCACCATTTATTGCATTTTTTAATTCAACTATAGCATCTTTAAGAGTTTTAATTGCTTGGTTTATTTCATCTATTGAAGAAGTGCTTGTTATTTTAGTATTTTTTAATGTTTCGGATGCTTTATTAGTATCGATGAATTTTGCATCTTCTGAATTGATTAAATCTTGCAATTGTTTTTGAATTTTTTCAAATTTTTCTTTTGCATATAAGGGATTTAATTTATTTTTTTCATCCATTTTTTGGATCAATGAATCAATAGCACCTTGAACTTCTTTTGTTGCTTCCTTGATTTCTTTTATTGAATCGCTATCATCAATCTTTGCATTATTTCAGACTGTTGTTTCGTTTTTCTTGTCAATATCTTTTGACCATTCTGATTCTAGCAAAGTCTTTAATTCTTGTTTTTTCGCTTCAAATTCTTGCTTTTGAATATTCTTTTCATTCTTAATTGCTTCTTTAAGTTCATCTAGAGCGTTTTCAAGATTTATTCTTCTTGTCTTAAATTCTTCAGATGATAATGTAGTATCCTTTGTTGTATTTTCTGCTAATATCTTTTTAGCTTTTTCGTTGGCACTTTTTATCATTTGAGCATCTTCGGTTTTAATTAATAGTTCCAATTTGTTTTTAGCGATTTTATATTTGTCTAATTCACTACTTTTATTAATTTTTTCTACTTCTGAGATTGCTTGTTTTTTGGCATCAATTAATAATTTAGTTGCTTGTTGAATATCGCTAATAAAAGAACTATTAATGTTTATATCGCTAACTTTTTCAATTGATTTTTCTGCCTTCGCCTTAATATCTTTGTATTTTAAATCATTTAAATCGTTTGCAATGTAATCTGTTAATTCTTGCTTTATTTTATTAAATTCATTAAATTCTTGTTGCTTCTTAAATTTAATTGCTTCTTGCAATTCTTTTGTAGCTTTTTTAATTGTTTGAACTGCTTGAAGAACTTGAAAGGCAGCAGATGAATTTGTTATTTTAGTATTTTTTAATACTTCAGTGGCCTTGGTGGTATCTACTGATTTTCCATCTTTTGAATTAATCAATGCTTGTAATTCTTCTTTGACGTTTGCAAAATCTAATCTTAGTTTATCAAGAGTTTTTTTATCGTTTTGATTTTGTTGTTCATTTTGTTTTCTTTCAAGAATTTTTTGAGTTAATGAATTAATTGCTTCTTTTACTTGCTTCGTTTTATTTTTAATTTCTTGAAGCGAAGAATTTTCATTTAATTTAGCATTATCTAAAATTTTAGATTCTTCTAATTTATTAACATTTTTAGCATAATCTGATTTTAGTAATTTTTCTAAAATAGATTTGGCCTTATTTAGTTCGGCCTTAGATTTTTCAATATTTTGCTTTAATTCTTGTTGTTTTTTAAATTTTCCTTTTGCGGCAATGGCTGCTATAGTAGCAGTTGTAGCTGCTGTGGCTAACAAAGAAATTGAAAGTAAAATACCTATTTTCTTATTCATGTTATTTATTTCTCCTTATCCTATATAAACTTAATAAATAATTTATTAATTTGCTTATATTAAGTTATTTTGAAACAAGAACCCTAAAAAAAAAAAAAAAAACGGTTTTCTCCACCCAAAGAGATTTTTTTGAACTTATTGGTTAATTAAATGTGATTTTTTTTATTTTTTTGAAATTTTTTTTAAAACAAAATAAATAATAAATATATAGATGTTTTTTAGCATAAAAGCCCATGAACATCTATAATAAATTAATCACAAAAATTTATAATTTAAAAACTAAATAAATCATTTTTTAATTATTTTAAATAATAAAAAAGGCTTGCTATTATTGCGCGCAAGCCTTGTTATTTTTTTAATTATTATTAATATCTTGGGATTATATGAACATGAATTCTGAATACTGTTTGATCTGCTGAAAGATTATTATTTATTATTAAATTAAATCCAGTTGCACCTAATTCCTTAACTCTTTTTAAAGCAAGTTCTCTAGACTTTAATATTAGTGATGACAATGTTTGGTCATCAATATCATATAAATTTGTGGCGTGGTTCTTTACTGTTACTAAAAAATGGCCTTTATCTACTGGTTTAATATCTTCAAAGGCAATAAATTTTTCATCTTCATAAATTATTTTTGCCGGGATTTCTCTTGTTATTATTTTTTCAAAAATATCATCATGCATATTATACTAGACCTTCAACTTTCAATATATCATAAACAGATTTGTTTGTTTCTGAAACATTTAATTTAAACCCTAGTTCATTAATATATTTAACTGCAGTTTTATATAATGACTCAAAATTAGCAGTCACTATATTATTTGCAATAATTTCTTGTTTTTCTTTCGTTGCTCATGGAGTTTCATCAAATGTTAAAACTTGTTTTGTAACTTCTTTGCCGTTATAATCTTTAACTTTCTTAGTTAAATATAATGGTGAAATTTTAACTACATATTGGGCAGTAATTGAATTGTCTTTTGTAACATTTATTCTTTGAGGATTTTGTTTTAAATTGTATGCAACATCTTGTTTTATTGACTCAAATTTCTTTGTATTTTTATTGTATTCTAGTTTAGCATTGAATTCATAGAAATTTCTTTTTCCTAAACCATCAACTGTTAAATTCATAGGAATTGTTAAAAAGTTTCCATCAAATGATATTGAACCTTTTTGGCTAAATCTCAATGTATATTGCTTGTTATCAAGAGTTATTGTCTTTGTATATTGATAATTATCCTTGCTATCTTTTGTTAAACCTTCAAATTCGTAATCTTTGTAATCAAACGATTTTAGTTCACCCTTTGAAACTTTAGATTGCAATGTTTCGGTTGCTTTTGCCTTTGGATGATTTATTTCTTGGGCAAGAATTTTTGCAAGTAAAAAGCTTTCTTGACTATAAACTTTTTTGCTATATGGATCTACAAATCCATATACTGAACTTAAATCTTTCTTTATACTATCTAAATTGCCACCTAAATCACCTGACGTATTTGAATTTTTAACTATGCCTTTATATGCCAATAATGATTTTAATACTTCGCCTTCCATTGAACCACTATTAATCAATTGTTCTGGGTTTTTAGCCGCTTCATTGTGACCATATTCTGGTTTAGTTGAAGGATTGTAAGAAGCTAGTTTATTAAATCCTTCAATTGAATTTATATATGATTTTGGTCATCTTAAATTCATATCGCGCTTATCATTAAATTCTCAATTTTCAATAATCGGATTTTCAACTAGATATTTCATTAAATACAATGAATCATCTGCGAAATTCAATGCTTCGTATAATTCTTTTTCATTAATAGGAGCATCTTTTTTAGACATTTTGTTGTGTAATGCAACTTGTGCTTTGTCTAGACCATTGCTATCATTTGCTATTTTTTTAAGTTCTGGTCTATTTTTTAATAAATAAAGTTTAGCAAGTAACATTTTTTGAAGTTCTAATCTAATGTTTGCCGCGATTTGATTTGAGTGTTTAAAAATAAATTTAAATTCTTGTTCATTTGGTATTGAAAATGCAGCAGGATTATAGTTAGTTGTGTCAATCTTTGCATTGATAAACAATGATTTTAAATTTCAAAAGAATTGAGGATCGGAAGCAACTCTATTAGTTGCATAATATTTGAATAATTGATAAAGATCTTTTGTTAGCGCTGAATTGTTGAAATTTGAGACAATATATTCAATCTTGTCTTTATCTTTAGAATCTTTAGCATAGCTTTCAATTTCATTTTTATAAAATTCTGAAATGTATATATCAGTAAGTCAAGTTAAAACCCTGTTTTTTGTTAATGAATCTTTTAAAACACGTTTTTGGTACCCTGAATTTTGTTGAGAATCTTCCTTTTCTTTTTTACATGAACTAGCAATCAAAGGAAGAACCGCAAGTGGCATTGCAATAGCTGTTAATAATCTAATTTTTTTAGTCATTGTATCTCCTTTTAATTTTTAGTATTTATGTAATTTACAATTGTTGTGAATAAATAAGATAAGATCTCTTTGTCTTCATGTGGTGATAGAATAAACTTATTGTTCTTGAACACATAATCTTTGTATAAATTACTATTAAATTTTTTAATTATAAATTGTTTTATTTCGTTTGCTTTACCATTAATTAAAACGTATTTACTTGTTTCGCGAATTAATTTTTCATAGTTGATTGCATTTAAAATATCTTGATCATTGAATTTACGTTTTGTTCCTTCAAGACTTAAATATTCTTTTGATTTAATATAATTTTTAAAATTATCTTGTTGTAATAAATCATTAACCATATAGTCACGAGTTAAAATTTCAGAAAATATTGAATCTAAATTAAACAATTTGGTATTTAATGTATCTGAGTGGTTGGCCTTAGATAGTATTGACAAATCATTTGTTATCAATCTAGTTACAACTTCTTCTTTGTCTAATGAAAATACATTTTGAATCAAAATGCCACTTTCATTGACTTGAATGTAATTTCCATTACTTTTAATTAATGTTGAAACCTTATACCCACTTGCTTCATCACTAAATGCATCTCGAAATGCCAAACCAAAAGATTTATTAAAATCTTTTTCACTTAAATCATTGATAAATTTTTCAATAGCTGCATTGTATCTAGTGTATTTTTCAGAGAAGTTTTCTTTGTCAACACTTTCAGTTCCTTCATTAAAAATATCGGCAAATTTCTTTGTTTTCTTTTCATGATCAATAAATGTAGCATCGCCAAAGATCTTTTTGAAGTTTTCTTTTAATTTTGTAAACAAATCATCTTTATCTTCAACTTTAAAAATGCTTTTTTCTTTGTCAGCATCCCCCATTAAGATTGAAAGAAGATTCATATACTTATTAGGATCTTTTTCTTTTAAACTATTCAACACACTTTTAAAGCCATCGAAACCGTATTTATCAGCTGACTTGCTATCACTTGAAACATATCTCAAAAGGTTGCTATCATTTTTGGCTGCAATTTCTTGCTCTTTAGTAATTTCATTTGTTTTAAGAAGTGAAGATATACCTTTAAATGTTGAAATGGCATCAATGCCTTGTTTAATTGAAACCTTGTCATCACTTTCATTCAAATAAGCAGAAAAACTTAATAATTTAATAATTTCTGCCTTTGTTACGACTCATGGATTTGAAGCATCTTTTGGATCTGGATGTGCCAATAAATTTAAATTAGATGTTATTGCTTGCTTTCTTTCGATTCAATTTTTAATGAATTTTAAAGGATTGCGATATTCTTTAACAAAAGATTTTGTAACAAACATCGGAATTTTAACTTCATCTTTTGTTCCAATTTTTAACGATTTATCATCAGATTTTGGCAAAACATAATTTTTATTTTCTATAGCAAAAGGAAGAGTAATTTTTTCACCCTTCTTAGCAATGTCAACTCTTTTGCCTTGATATGTGTAATAAACGTCTTTGTTTGCAATAATTCCATTTTTTAATTCACTATAAGTAAAATCACTATTTACTTCGGTTGTATATCTATTAAATGAATATCTATTTAATTTTTGTGCTAGAAGGTATTCAATTGCTTCTTCTTTTGACTTTGCTTTTCCATATCTATCAGTTGCAAGTTCTTGTAAGAATTTTTCTTCTCAATTTTTTTGTAGACCAAATTGTTCTTGATATTTCTTTTCAAGATCTTGAATTTCTTTTGTTATTTCTTTTTTAACTTCTTCGATTGGTTTTAGTGCTAATTTTTTAATATCTTTTTCTGCCTTATCAGCATTATAAACAGCTTCATATCACAATGAACCTTCATATTCTTTTTCATAAAGGTATTTAATTAGTTGCTTTTCAACACCTTCAGCAGCATGTTTTGATGAATTTGCTATTTCATGGATTTTATTAATATCACCATAGTTCAAATTTAACCTTTGACCATCTGGCCCGGTTATTGATAAAATTGAAGAAGAATCTTTTAATAAAGGTGAAGGCTTAGGTAAGGAATGATTAGCTTGTGCTAACGGAACGCCAATTCCTGCAGCAACTGCGGTTGCTATTGCTGTGCCTCAAAAAGCACCTCAAATAATTTTTTTACGCTTAGCCTTTTGTTCGGTTGTTTTGGGTTGATTTTGATTTGACATAGTAAACCTTTCTATTATTTAATATTAAATAATTATACTATTTTTGGTTATTTTTCTTTATTTATGGATATTTAACCAGTATTATTTTATAATTTAAAACATGAATAAATTACTTTTAAATAAAGATAGAATATTGCTTGAATTTATTGAAATTTGTAAAAATCTTAATCTTTTTTATAGTTTAGATAAATTAAGCTTGTATGGCACTATGTCAAAAGAACATTCTTCAAATAATTTAGATCACCATGAAATAATGATGAAATATCAAGATTATGAAATCTTAAAAAATGCTTGTCCTAATAGAGTTTTAGATAATACATTGCATTCTTTGTATCACGACTTACAAATAAAATTTGTTGATGATAACAAAAACATATTTGAAAACCAACCGTTTTTGAACATTAACTTAATTTATCCAACAAACATTGCCAATATAAAGAAATATTTAAAACATTGCAATCAAGCTAGATCATTTATTGGCCACTATTTAACAATGACAAATAGCAACGATTGAAAAACAAAAAATAATATTATTAAATCTCATTTTCTATCAATTTTTTATAAACCAATTACATACAAAGAAGTTGCTAATTTATTGATAAAAAATGATCACCAAGGTTTTTTAATCTCAAGTCCGATAATTAAAAAAGATTCATTGAAATATTGATTGCCAAATATTAAATTTGAAACAAAAAAAGTAATTATAAACAATATTGAAGTTGATGTTTTAGTTGAATTTGACCAATATCTAAAAAACGTTTATGAAGTTGAATATTTTGAACATTCCGTTCCACAAAGAAAGATAGAATATATTAACCCCATTGAATATAAAAAAATATTTAGCTAATTTTTTATAAATGAAAGTTCAAAATTGCTTTTTATTGAATAAATTTCAAAATTTCTAATGTGTATTGAAATGTCTTTGTTTTTTTAAAAAATAAACAAATAAAAAAGTAGGAAGTTTCTACTTCTTACTTTTTTTAAAATTGACTTTCTCTATCGATTAATCATTTTCTTTCTTGAAGTTTTACTTCTTTAATTAATCCCAACTCCAAGTATATAACTTTATCAGCAATTTGGGCAACATCAGGGTCGTGGGAAACAATGATAATGGTTGTGCCATATTTTAAATTGATTTGTTGCAAATTTTTCAATACAATAATTCCTGTTTTTTCGTCCAATGCTCCTGTTGGTTCATCAGCTACAAGAATTTCGGAATTTTTTGCAATGGCTCTTAAAATTGACACTCTTTGTTGTTGACCACCAGACATTTGAGAAGGATATTTATACATACATTCAGAAAGATCAAAATCTTCAAATAATTTTTTAATATTTAAACGCTTGTTTTTATCTCTTTGTAAATATGAACCAGTTTCAACATTGTCATAACTATTCAAATTTTGAAGTAAATTATAATTTTGAAAAATGAACGAAATGTTTTCTCTTCTAAATAAGGTTTGCTTTGCATCAGATAAATAAGGTAAATTTATGTTATTTACAATTACATTGCCAGAGGTAGGCCTATCAAGAGCACTAATTATGTTTAATAATGTTGATTTACCTGACCCAGACTTACCATATAGAACAACAATTTCTCCTTTTTTAATTGAAAAAGTTATTCCTTTTAAAACTTCAGTTGCTACATTGCCGGATAAATATGTTTTTTTAATATTATCAACTTCAACAATGTTATCGTTAGAATTTTGTAATTTAGCATACTCGTCTTTTTTTCTTGGTTTGTCATTAGCAAGTCTATATTTCTTTAAAGTTGCATTATCTAAAACCTTAATTGCAATGCCTTCATCTTTAATTTCAAATGGCTTTTTTTGCTTAGAAATATTTATCAAGTTTAAATCTTGCAATGTTTGTTCATCAACAAAATTTTTATTCTTAGAAATTTTATTTGTTTTAGCTTTAATTTCTTTTTTGTTTTCTTTTATTTCTTTTGCCATTTTATTTTCCCTTCAAAGCATCTACCGCTTTTATTTTATTTAAACTGTTTCATGTTGCAATTGATGTTGCTATAAATACTGCCAAACAAATTAATGCACTTAATATTACTGTTGAAATATGTAAAACCAAAGGTAATACAATCTGGCTAGTTACCATCATAAACGAGTTGAATATTGCTATTATTGCAAGAGTTGCAGGAATCATTAGTAAGCAAGCAAATACAATTGCAGGTACAAAATTGAAGAAGAATAATAGAATTTTTTCTTTATTTGTATATCCCAATATAGAAAATGTAGCAATTGATCTTCCATTAGAAGCAATCATTATATTAGTAATAACAATTAATATAACTATTGAAATTATCAATGAAATTATTATAAATGCCAGCAATATGGTATTAACTGTTGAAGAAATTCCTTCAATAAATCCGGCTTCAATGTCTTTACTATTAACATCAAATGATGCACCATACATTATATTCTTGCCATATATTGAAGATGCTTGTGTGCCATAGAATTTGGTTAATGTTTCTCTTACTAAGATTTTATAACTTTCTAATGGGCTATCTGAGTCTATTATTTCTTGTATACTTTCATCGTTTAGATTTCCAAGTAATTTCTTTAATTGAGTTTTATAATCTAATTTTGCCTGAGGATGTGCTTCATTATATGATTGAATATTATGTTCATATACAGAAATATATTTTAATTTTGGATTGCTTATAAACATGCGTTTAAAGAAATCTCATGCGCTATTGTCATTTGCTTCGGCAACATCATATGAACTTGCTGCACCTCAGAATCCTGTTGCTGAATAAGTTGTTAATGTATCAATAGTTTGAACTGGAACTTTATCAATTGACAAAATACCGTTAAATGCATCATATTTCTTATTGAATTTTTTAATATATTCATCTTTTAAATCAGGGTGTAAACTAATTGCTTTTTGTAATTCGTATTTTCTTGCATCTTTCAATCTTTTTGAAAGTGTATCATAACCTAAAATGTGGTCTAAAATATCTTTCCTTGTACTAATTTCGGAGTTAATATAAGTATCTGATATGCCAATTACTTTAAACTTATAATCGACTCTTGGGCTTTTTTGCATTAAAGCTTGATATGCAAATCGATCAACGTGATTTAATATTTTGGCATCAAATACGGAATTAACTTTTAATCTATAGCTTCTTGCCGTTACTTCATTTACAACAACAGGAATTGGATCATTACTATCAAAATTGTAATTATAGTCTTGTAATAATTTTGTTAAATTATTGCCCCGACTATCTTGCAATCTCATGAATTTACTATCATTATAATATCCATAAATTTTAGTTTCTTTTCCATTTAGAGTTACTGAAGCATATGTGTATTTTTCGTTGGTTGTATCGTTTCAATAAACTCCACCAAATGAAACATAGAAATCTATACCATTTATTTTTCTATAAGCATTTACTAAAAAATTACGATATTCTTGTCTGAATTTTGCTGTACTTACTTTGGCGGGTTTTAAATAAATTTCATCTTCTGCAACTCATTCAACAAATCTAAATTGTTTTCTATTTCCTGATGCTGCTTCTGAATAATTACCAGCGCTAAACAAGAAATAACTTGTTCTATTTGATAAATCTTCTGGACGTTTAGCATTATAGTCATCAAAGAATTTTTTAATATCTTTTACAGCAATTATTTTTGTTGGATCTTTTACATCTTCTTCATCTATTATTCTATAATCTCCACCATTTTTTAATCTACGTAAATTTATTAAGTTTTGAGTTGATTGCATCATTGAACTTACGCGGTTAAATATTTGGTTTACTCTTGCCCTTTGTGTTTCAGGCATGTTGGCATAAGTTATATCTCATGGACTTAATTCAACTGATAAATCCATTAATAAATCAAGCGATGATTTTGTAATGACAGTAGGCGCAAATTTTCTTTGAATTACATCTGTATTAAATGAAGCTCCAGGTCTTAAGAAATTAGGATTATCATAATCTAGTTGACTACCATTTGTTGATGAATCACCTGCAAGATCATTAGGAACATACAATAAATCATTTATATCGTTTTGATTGTATGTAACATAAGGACCACCTTCGGTTGTTGGACTTTCAAGATTTAATTTATATTTATATAGCCTATTTTTATATGTCTTTGAAATAGTTTTATTAAATACGTTATTTGAAGATAAGAAGAACATAGAAATTAATGAAGTCGTTGAAAATGCTAAGAATAACGAGAACATTTTTCAGAAATTATTCAATGCCATCGAGGCAATAAATCTTGATTTAACTGGCATTTTTCTAAACATAGCAGAAACTCTTTGGGCAAAATTACCAACACTAACTTCAGTTAACCCACTCATTAATTCTGTAGGTTTATTTCTTACAATAACTCTAGTAATTATATAAATAACTAGTGAAACAACAATCAATGGAATAATAACTACTGCTAATATTAATAATATATTAAATGGAATAATATTGTTCTCTAACGTTCAATAAGAAGATAATATTGACATTGCAGGTTTTTGTAAGGCAAATCCCACAATATAACCAAATATTCCACCGACAATAGTTGGAATTCAGCCAAAAGCACAGAATGATAAAGCAATTTCTGATGTTTTATATCCTTGAGCTCTTAATATTCCAACAACCTTATTTCTTGCTTCAATATATCTTTTGATAATAAAGTAAACAATCAAAATTACTAATATTGCCAAAATTGTTATTAAATAAAGTGTTGCATTTTTAATTGATGTTACTAATGTTCTTATTGTTTTTACTCTGATGTAACGTTCGGGGTTGATTGCATCTGGTTCATCTTTTAAAAATACTTTATTTTTTGAATTTGGAGAAATTGATGAAACAATTCTTTGAAGTTCTTTCTTTAAGTTTTTAGGATCTTTGCCAGCTAAATATTTTCCTTTTTTGTCAACAGGAGATTTTACTAATGCATAACTCTTTAAGGCAAATGTAGGATATGCCGATCTAATTCTGTCAAAGCCTCTTGAATTAACATAAACAACAGATTGAGTTTTTGTATCAACTTGTAAGTTTTCTTCATTAATTACAGGATATAAATAATCAGCGGTGCTATCAATACCTGTTATCAAATATTCTTGAGTATTTACTTTAATCTTATATTTATCATCTAATGAAGCAATGAATTGTTGCATTTCAAATGGATTATTTAAATATTTCGAAATATCGCCATTGTAAACTTCTTTTTTATTTTTGTTTAAATATCCATAATTTACCTTGGCTAAATATGAAGCAGCTTCGGAATATGCAATTAAATTATAAACTGTGCTTGGTTCTATTATTTTTGAAAATACTTCCTTAACAGTTTTGGTAATATTTATTCCAAAAAAGCTAATATCTTTGTTATCGGGTGTTTTTAGTAAATGGTTAAACATATAATTGAATTTAGAAATATCTAAATCAATTCTGCTATCGCTTTTTGAAGTAACAACATTTTCAACAAAATTGAATTTTTGAGTTGCACCTTCGATTTGTTTTTTCAACATTGTTGCATTAGTTTCATCAATATTCGATAATAATTTTTTGATTATATAAGCAATATTTCCACTATCTGGGTTTGGACTTAATACGTAGTATAACAATATTTGTGGGAAACTTAAAATATTGAAACCATTTAGAATATTTCTTGGCACGTTTAACAACGAAATTATTGAGGCGGTTTTTGAAGCTGCAATTGCATCCTTATATACTAAGCTAAAGGTATATTCATCATTTATTAACGGTTTAATTTTTTGATTTTGATCATTGACATTAATAAATGATTGTAAAAATAATTTTAAGGCTTGAAGATAATGAGTTGGCATTTGATTTGAAGAGGCCATATAACCAAAATTGTAGGTTATTTCATTCATATTATTTTTTAATATCTCTTTAATTGCTTCAATGCCCTTATTAGAAACATGATTTTTAGATAATTGGTCAAATAAATTTTTAAATACTGCTGAATTATTCATTAAATCCATAATTTGCTTTGTTGCTTTTGCTGTATGTGCGTGAGCAAAAGGCATTTTAAATAATTTAGCAGAATAGTCATCGTCCGAGCCTAAAATTCCAAGACTAGGAATTGGATTAATTACTTTTTTGTAATTTAATACTATATCTTTAATTTCCTTTGATATTCCTATGTTGTAAAGACCTAATAATATCTCTTTAATTTGCTTGTAATCTAAATATTTTGTAACTTTTCGTTCAATTATTCTCTTATTAGTTGTTGGATCAATAACAATATTTTCTTGAATATCTGCAATTTGGCTTAGTTGATATGCAAGTTTAATTCATAATGAATATAGAGATAAGACATTAGAAGCAACATTTTGTGGATCATTATTCAACATTAATTTTGCTAAATTGTCTTTTATTAATGAATGAATAGTTTTTAAAATTTCCAAATCTTTTGCATGTTCAGGCGCTACATTATTAAATGAAGCCGAATAATATGCAACATCCAAATAAGATTTTAAATCATTTAATTCAACTGCTGAATTTATACCGCTTTGCTTATATTGAGAGTTTGATGTTACAAGTTTGTCTATATATTCATGATATTTAGTTAATTTTGAAATTATTAAATCAAGGTCATTAAATTCGTCTTTGGTAACTAAAACATTGTTTTTTAATACATCAAATTCGTTTTGAGTTAATTTCAACTTTTTGTTATTTATTTTTGCATCTTTTGCCTTTAATAATATTTTTTTAATATCATCAACATCAAAAGGATTTATTGGAATATTAGTCAATACATTTGTTTGCTTTTGAGATTGAGGGAATGAAAATTTTGACATGTTTATTAAATCAAACAAAGTAATTTTATTATTGTCATAATCAGGAATAGTTATATTCAATTTACCTAAAATTCTCTTTGTCTTATTTGAAAAATTAAACATTTGAATAACGGATTCTTGAATTTGGGAAATTTTTCCGCTATTTATTTTTCCATCTAGGTTTGAACTTATTGCACCAAAAAATGATGTTAAATAATCTGAACTAGTTAAACTTTCTGTGTTGAAATCTTTATAAATTTTATTATTTGCAACAATTGGATAATGTTCATGTTTAACTAGTTGCTCCAAGATTTGAATAAATCTATCAAGATCAAAATTAGCTATTATATTTTTTAATCCATGTTTAAAATTATCATATTCTTGGCCTGCTTTTGTTGCACCGTTTAATTTTCTAAAAAATTCTTTAATTCCGTCTTTTGTTTGTTGGTCTATAAAATTATTAGCTTCTCTATTTGCATTAATTCATTTTGAATACAAACCATTAGACGATTCTGGATTTAAAAAGCCATTAAAGTCAATATTATCAATCAATTCAGACAATGTTGATTTTAATTTGTAAACATCAATAGATTTTAAGAAACTTATTAAAATTCTTTCCGATGATAGTATTCAATATGTATCATCAACACTAGTAAAAGGCTTATATGGGTGTTTTTCAAATCAATCTTGAATAATTAAAGAAAATTTTTCAAAATCAACAGAGCGAATAACATTTCTCAATACTTCAAAAATTGTTGATTTATTTTTAACTAAATTAATTATGTCAAATAAACTTAATTTCAAAGAAGGAATCAAATATAGATTATCAAGATTTAATAATTTACTTATATTGTAAACTTGTTGCATTAAATATTGATTTCGTTCATTTTCATTGGTTCCTGAATTCATATACATTTTTTCTAAATAGTTTATTATTCCAATGAATACATTTTGAACAAAAGCATTAACATTAACATTATTAATATTTAAATTGTTACTATTAGTTCTATCTACTGGTCTAATAATGTATTTCAAAATATCTAAAAGAACTTTTTGTAAAATCGTGCCATTTACCTTGCCAATATTTAGCAATGTATGGAAATCGTTATCCACAACACTTTGTTTTGCTGCATCCATGATTCTCAATAAATCATTTTTATTGAACATGTTAATAATTTCGCTTTTGAATAAAGCATCTCTAATAACATTAACTAAACCATTTATTGTTTTATTTTGAATAATTTCGTTGGTTAAATTGTTGTTTATTGCCCCGAAACTTATAGGCAAACTAAATGAGTTTTTAAACTCCTTATCAGCAACTGCTCAACCATTTGGACCAATTTCTCCTCTGATACTATAATTATTTCTAATCATGTATTCATAAAGTTCTTGAGATGTTAAATATGCCTTTCCATTAACATCAACTCTATTTCAAACTTTTTTATCACTAAATCCATCAATAGTTGAATATTTTAATAAAATGTATTTTTCATCACTAATTCTTGCAACTGCACCAACTATTGAAGAGCCAACGGTCTTTGGTTCATCATTAGCAACGGTTAACATCAATATTTTTCCATCGATTTCATAAGGTATTTTTGTATTTGGTAGGAAATCATAATATTTTAGAAATCTGATATCTGCATTGAAATAATTAATAGACGGCGTTCATTGCTCAAAAATATATTTAATTATTTGTTTTGTATAAACTGGTGGAATTTTTTTGATCATTGGATCGTTTGGATCGGGTTTCAAAATAAATTCATTAACATTTTTATCTGATACAGAAGAATTTAGTAATGTAGGATTTAGAGTTTCGTTATATAATTTACCAACGTTATTTTTTACGCCATAAATTTTTTGGTTTCCGTCGCCTGCATTAACAAAGTGAAAAACGTTTTTCTTAGTAGTTTTTTCATCAACGGATTCTACAGTTAGAGTTTGTCTAATTCCTAAGTTTTCTTCTCCAACATTATTTTTTATTTCATTAACTAAGGCATTTCTAGCATATGAAACAGACCCTTTTCTAATAAATTCTTGATATTTTTTTAAATTATTAGGATCTGATATTGCTTTAAATTGGTCTTCTGTAATTAATTTATCACCGGATTTAGAATACTCAATTTGTTGAATGTTTCAACGGTGTTCTTTAGAAATGTTTTCTTTTATAGCAGGAATTTTGGAATTATTAAATCCACCATTTCAACTGCTTTTAAAAGTTTGCAATAAATCTTGTTGTTCAATTGGCAATGTTGTAGTTTTTTCTTGTTTCAAAGAAGACGAATTTAATTTATAAATTGTTTTAATCGTCTTATTTCAATATGAAAAATATTGAATATTTTTATAAACATTTGGGTCACTTTCTTTTAATTTAGCAATGTATTTGAAATATGATCCTTTTCAATTTGAGCTATATTCGTCACTCTTTTCGTTTCAATTTAATGCAAATCTATATCAATTATATGTTGTTAAAACCTCGGTAGATTTTATTCATTCATTTGGAATTTGATATATTTTATTTGCTGCTAGTTTTGTAAATTCATTAACATCTTTTTCAATACTTTTATTGCTTAAAAAATTTTGAAATTTAAAAGTTCTTGAAATATTAAAGTCTTCATTAATAAATTTTGATTTTTGACTTGTAAAATCCGAATTTTCGATTTTTAAATCAGAGTTTTTATTATAAAGAGATGCATCAAATTCATAATGGTTTTCTGAAATTTCTTTAAAACCAAGCATCTTCATTACATCTCTACCATCAATAGTGTGCAATGAACCGCTGTTTTTTCATGCTTCATAATCTTTAGTATCAAATGATGCTTGTTTAGTTTTAACATTTAGAAATAATGGAGAGGGATTGTATAAATAATCAACTACTTTTTGATCGTTTAGCGAGCCTCTTGGACCATATGCAATAGAAGCTATATTTTTTAATCTAACTTCATTTAAAAAAGTAATGTCATCATTTTTTGTCAAATTATATCTTTTAGTTAATGGTTTTATATTTCCTTTGCTATCTTTTACATAAAGTTTAAATTCCCTATCATGACCCGAAAAACTAAAATTCCTAATTTTAGAAAAATCAGGGTTGGATTTTAAAATTTCAACGTCTACATCACTTATAGATTCCTTGCTAGAATATAATAAATTTAATAAATCATAAGTATTTATAAAATGGTTTTCATTATCATAATTTAAATTATTAAAAGTATTTTTTAAACCTACATAATTTCTTTGATCTTTTGGTAGATCTATTGAATATGAAATATCACTTCCATTATTAGAAGACTCAAAAACAACAGGTTTATCAACAATTGATTGATTATCAGATCCTATTTGGTTATAACCTGATTTTGGTATCACACCTGAAGGGTTAACGTCAAAATCAACAGTTGCATCATGCAATTTTGAAACCTTTTCATACGAATTAATCGTAGATGAATAGCTAGTAGTAACATCATTTAACAAAGTAAATAAAAATGTTGTTAAGAAAATTAAAATTGTTAAACAAATCAAGGTAACTTTATTTCTTGCCAATGATTTGAAAACCTCTTTAAAAAGTCTTCTCATATTTCTTCTCCAGTTTAAAAAATATAATTTTTTAATATATTAAAAATATAAAAATATGTGTATATTATAAATGTTTAAATCTGAAATAACAAAAAAACAGCACTTTTAGACCAAAAAATGGCTTAAAAATGATGTTTTTTGTTAATTATTAGTTATTTTTTAGATTATTTTTAATAATTAAAGATCATTTTATAGGAAATCCTAAGGCTGTTTTTATTAATTTTTGGTGAAAAATTAAAACATTTTCAATATTGAAATATATTGGCAAAATCCTTGTTTTTAAATTAATATTTAATTTGTTAACTATAAAGTTTGAATTAGCTATTTCTTGTTGATATTTATTTGATTTTAAAAAGCAAGCAATTCCATTAATTTTTAAAAGATGGTGTGAAATTTCTAATAAATTTTTTAATTCACTCACCGCTCTTGCACTAATAAAATCAAAACATTCTATTTGATTTGAATCTTCAGCACGAATTTTTTGTATATCAATATTTTTCAATTTCAACTCATTTTTAACCAAATTTAAAAAATTTACTCTTTTTGATTGAGGCTCATAAATTGTTAAAGAAAAATCTTGTTTATATATGAAATATGGAAGGATAGGAAATCCTGCGCCCGAGCCAATGTCGAGTACAGATTTGTTAGTTAGATTACAAATATTTTTTTCAATGTCTAAAAAAATTAAAATTGATTCAATTATTGCTTCTTTTAAAAGCTTTTCGTTATAGAAACCTGTTAAATTATATTTGCTATTTTCTTGTTCTATTAATTGATAATATTCATATAATTTTTTTAAGACTTCTTTGCTACATTGTGGTAGACTTTGCAATATTAAATTATTGCTTTCTTTTTCCGTAAGCATTATATACTTCTGTTATAGATTTGCTTTCAATGTTTGCATTGATTACCATACTTAAAAATTGAGCCAAAGATAATTCTCTTAATTTTTTAAAGTTTGATAAATGGCTGTGATCTATTGAATTAGTAATAATAACTTCATCAATCATTGCGTTATTTTCAAACATTTCAAATCCTTTTGAAAATAAACCATGAGTGGCTGCAATTATAATATTTTTTGCGCCATTTGCTTTTAAAGCTTCTGCTGCATGAATAATTGTTCCACCTGTGTCAATAATATCGTCATAGATTAAAACATTTTTATCTTTAACATCACCTAAAACACCCATAATTTCGCATTTATTAGGTCCTGTACGACGCTTGTCAATAATTGCAATTTGTTCTTCATTGTTTAGTAATTCTGACAATTGTCTTGCTCTAACTGCACCCCCGTGGTCTGGTGATACAATTACTAGTTTTTCTTTTCTGTTTAGCAATTCATTTGAAAAAATAAATTGTCCCCTTAAATCATCAACAGGAATGTTGAAAAATCCTTGAATTGAAGCGTTGTGCAAGTCAATAGTAATTATTTTATCAGCACCCGCTGTTTCATACATCTTAGCAACTAGTGCAGCTGATATTGGATCTCTTCCACTTGCTTTTCTATCTTGTCTTGCATATCCATAGTAGGTCATAACGCAATTAATGCTCTTTGCTGATGAACGTCTTAATGAATCAATTAAAATCAGCAATGACATTATGTTATCGTTTACTGGTTTTGAGGTATTGCAAATAATGTAAACATCCTTATTTCTAACGGTTGTTTCTGATTTTAAAAGGCATTCTCCATCTGCAAATACTGTAGTTTTAACTGGCAATATTTCTTTATTTAAATACTTTCCAATTTCTTGTGCTAATTTTTGGGAATTTTCCATGCCAAAAATTACAACATTATCTTCTTTTTTTGCTTTCATTTAAATCCTTATATATATTCTCTTTAATTATTTATAGTTAAATTTTACATTATTTTATTTAAAAATAAAATATTAATAAATTGCAATTAAAAAAAGTGCAAGTTGCACTTTTATAAAAATTCTTTATATATACGTTTATATATTGACTTAACACTGACAACTAATAAGACATATATTCCTAAAAATAAAACTAATCAAGCATAATAAATTCCTGGTAATGGCATTAGACTTAAACCTTTATTAATTCCTGGTATATATGGCACGGCATTAATTATTGCACATCCAATTAATGTTGAAATAAATAACGAAGTTGTTGCATTTGATTTAATAAATGGTGTTTTGTCAGTACGCAAAAAGTGAATTACAAGGGTTTGAGTATACATAGAAACAACTAATCATCCTGCTCAGAATAGTTCTTTAAATGCTTTTTGTTTTTCTATACTTAGTTCATCTTTTAAAGAAGCAAAGCTCTTATAAGCTCCTATTTGTACTCAATCTCCCTTACTAGTCATAAATGACGCATTTGGAATTATTGCAAAGAATAAGACTACATATGAAACAATATCTACTATTGAACTCGTTGGACCAAACCAAAACATAAATCTTCAAATTGATTTATACTCTCATTGCCGAGGTTTAAGAATAAATTCAGGGTCTACATTGTCTCATGGCATTGTTGCACAGCAAACATCATATACCAAATTCAATAATATTATTTGTACAGGGTTTAATGGAACAAAAGGAATTAAAAATGAAGCAATTAAAATACTAAATATATTTCCGAAGTTTGAAGATGCAGTCATTTTTATATATTTGTTAATATTGGCATGTGTTTTTCTACCTTCAACAATTCCTTTAGCCAAAACGTTTAGGTCTTTTTCTAAAAGAATTATGTTAGCTGATTCTTTTGCAATATCAACCGCAGTATCGACTGATATTGAAACATCGGCCGATTTCATAGCGGGTGCATCATTAATTCCATCACCCATATAGCCAACAACATGACCATTATCACGCAATATTGAAATAATTCTTGCCTTTTGGTCAGGGCTTAATTTTGCAAAAATTTGAACTTTTTCAACTTCAATTTTAAGTTCAGCATCACTTAATTCTTCAACTTCTTTGCCTAGTAAAATTCTATTATTTTTTATTCCTACTTGGGCACATACTGCTTTTGTTACAAGTGCACTATCTCCTGTTAATATTTTTACATCAACTCCATATTCATGCAATTTTTCAATTGCAGCAGCAGCAGAATCTTTAGGAGGATCAAGAAATGTTAAATATCCAATCAACGTCATATCGCATTCATCATTTACCGATATTTTTCCAACTGCATTAATTGTTGATTTTTTAGAAGCTATTGCAATAACTCTTAGTCCTTCTGAATTGAATTCTTCAACTCTTCTTAGAACCTTATCTATTGTTTTTTTATCAAGAGGTTTTACCTCTCCATCAATGTAAATATTTTTACATACACTCACAATTTCTTCGACAGCACCCTTGGTAATTAGGGTTACTTTATTTTGTTTTAATGATTTAACTAAAACAGACATTCTTTTTCTTTTGAAATCAAAAGGAATTTCGTCAATTTTTTCATAAAATCTTTGAAAATCCTCAAGTTCTTTATTTTTTGATGCCATTTCAAAAGTCTTATTAATTATTGATTTATCAAGTAAATTTTTTAATCCTGTTTGATAATATGAATTAATAAAAGCATATTTTAAAACATGAAAATCTTCTTTGCCGGTAACATCCATATGGCGTTCCATAATTACTTCATCTTGTGTTAATGTTCCAGTTTTATCTGTACATAAAATGTCCATTGAACCAAAATTTTGAATTGAATTAATATTTTTAATTATTGTTTTGGCCTTGCCTAGTGAAACTGCACCTTTAGATAAACTTGCTGTCACTATCATTGGAAGCATTTCCGGTGTTAAACCAACAGCAATTGATATACCAAACAAAAATCCTTCTAATCATTGATTTTCTCCACCTATAACACCTTTAATTCCTAAAATTAAAAATACTATAGGAACAATAACAGCCATTATAATAGTGAACATTATTGAAATTTTTTTAATTCCTTTATCAAAGTTGGTTGGTATTGGTTTTTCATTGATTTTAGAAGCAACTTGTCCAATAATTGTATCATTGCCGGTTGCAATTACAATAGTTTTAGCAGAACCTGAAATAACATTAGTTCCCATAAAAGCTAAATTACTACGGTCAGTTATTGCTTCAAATTCTTTAGAACTGTCGATGTTCTTATATTTTTCAATAGCATCGCTTTCTCCGGTTAATGAGGATTGAGAAATAAATAAGTCTTTTGCTTCTAATATTCTTACATCCGCCGGAATAATATCTCCAGCTGCTAAATACACAATATCGCCTACTACAATCTCATCCATTGGAATTTCCTTCATAATACCATTCCTTTGAACAAGCGAAGTGGTTTCAACAGTCTCAACTAATTTATCAGCGCTTGATGCCCCACGTTGTTCTTGAACGAAGTGAATAATTCCACTTAAAAAAACCATAAATAAAACAATGATGACAGTCATTCCATCAATTCATGTTATCTTTTTGCCTTTTGATGCAGGCAATATTAAATCAATAATCATAGAAACAATTGCCAATGCAAACAAAATGATGTTAAAAAAATTAAAAAATGATCTAAATAAACGCTTTCAAACATTATCAGCGCCTTTTTTTGTTATTTTATTTAAACCATATTCTTCTCTTGCAGCTTCAATTCTTTCTTCATCTTGAAATCCGTTTTGAGAGGTTTGATATTTTAAAAATAATTCTTCTTGACTACAATTAGAAGCTTCAACTAACCTTGTATTGATTTGCTTAATTTGTTTTTCTTTTCTTGAAGAATTTTTAAGTTTATTTTTTGTTATATTAACTATCATGTTATCTCCTTTTTATTTGGGTATTTATTATAGGAGAGCGAAATATAATTAAATACTAAAAGTTACTATGTAATTTTTAGTTAAATTGCTATATCGCTCACAACTTGGTAATTCCATAGTACCTCCATATATTTAAGTTTATAAAAGTATTTTAACATATTTCAAAATTAATTTCATCTAAACTTAAAAATTAAAAATTATATAAATCTTTTATTTTTAATTCATTTAAATTTTTTGTTTCACCAATTTCAATAATCTGTCCATCTTTGACATAAATGTATTTCTTAAAATAAGTTTTTATTAATGAAATGTCGTGAATATTTACTAAAATTGTTTTTTTGTATTCTTGTGATATTGAAGACAATAATTCTAAAACATCTCTTGCGTTTGCTATATCAAGACTATTGGTAGGTTCGTCTGCTAAAATAATGTCAACTTTTTGTAATAAAATAAGTATCAACCACAATCTTTGCCTTTGACCCCCGCTTAATTCATCGACTCTTGTAAATAACTTATCAAATATATTGAATTTTACGAAATATTCATATATTTCTTGTTTTTGAGTTTTGCTTAAAACATGAAATCAAGAAAAAAGAAAATTTTTATATTTTGGATAAAAATGTAGAATGTTTTCATAGCAATTTAAACTTTCAATAAAATTATTGTTTTGACTTAAAAATCCAATATTTTTTAATATTGCCTTTCACTGTTTTTTTGAAGCTCTATAAATATCAATATCATTAATTTTTACATTGCCTTTTTTTACAAACAATTGCTTCAATAAAGCATTGAAAATTGTCGATTTTCCAGCGCCGGATTTTCCTATTATCGCTATAAATTCGCCCTCTTCAATAGAAAAATTTATGTTCTTTAAAACTATTGGACTTTTTTTATAATAACTTGCATCAACATTGATGAATTCAATTTTTTTCATATTTAAATTATATTTGGAATGTATTAATACAAAACAAAATATTGCAAAAATAAAAACATTAAGCGTGTTCTGCTCAATGTTTCTCAGCAAGGCTTGTATCGACCTCATCAATTTTTATTTAATAGTAATTTCAACATCCAAATTTTTTAATACTTGAATTGCTGTTTCTTTAATTTCTTTTGAATATTCATCAAATTGTTGTTGTTCATCTTCGTCAAGTCTAATTTTTGGCAAGTGTGTATAACCATTCTTACCCAAGATTACTGGTAAACCAAAGTAAACTCCGGCATGTTTGTATTCCTTTGGTAATTGAACTCCAAGAGAATAAATTCTTTGTCTATCATACAAAATTGCTTGCGTAATTTCAGCAAGGTTTTCGCCAATACCATAATTAGTAATGCCTTTGCCAGCAATAATTTCAAATGCTTCTTTTTTAACCATTTCATTTAATTGAGAAAGTCATTCATTAGTTATAGTACCATTAGCTAATCAATCTTTTAATGAACCCAAACCAATTCTTATTTTTGAAAATGGAATTAAACATCTTGCTCCATGTTCACCAATAACAAATCCAGTAATTGAGTGTGCTTTAATTCCAATTTTTTCTGAAAGGAATTTTTTCATTCTTGCAGTTTCTAGAATAGTTCCTGAACTTATAACTTTATTTGGAGAAAACTTGGTTACTTGTTGATAAATTGTTGCCATAATGTCAACAGGATTTGAGGCAATGATTGTAACACCATCAAAACCTGAATCTTTTATGCTTTGGCCGATTTTTCTCAATAATTTTGCATTATCGGTCAATAATTCCATTCTATCTTTTAAAACGCCTTCTTTTGGTCTAATTGATGCAGTTATAATAACAATATCAGCATCTTTCAAATCTGAATAATCTCCAGCTTTGAATGTTGAACCATTGTTGGCCATTGAACAAGCAGCATCATTTAAATCTTTCGCTTGCGCAATTGCGACTTGTGGAAAACTATCAATTAAAACATAATCTGCTTCTATATTTTTATTTAATGCAGCATATAAATATGAACTACCAACAGCACCAACGCCAATTAATCCTATTTTAGTCATTTTTTTTGCCTCCTAATAACTCTTTAAAATTTTTTGCCAAATTAAAATTGTAAGATTCCAAAGGCTTTCTTTCGAATGGATTCTGTTCATTTAAATAGGCAAATAAAATACTTGAATGATGTAAGAAAGACATTATTCAACCCAATGTACTATCGTTATAATTGTCAATCATGATTTGAATGTTAGGTATTTTATATACATTAACATGGTTTTCAATAAATGTTTCCATTACAAGATTACATAAATTATTATAAGTTAAATTGCTTAAATAATTCAAACCATCCGTCTCATTTTCGTTATTTTGTGAAATTTTGCAGTCCATAATAGGTTCTTGAAATTTTAAACAAGTATCAAAGCACTTAAATGGAGTGTTGGTGTAATTTGAGGCCAAAGTTTTGTTATCAACGCCCCCTAAGCAAGTTTCAATATAAATCCTATTGTTCATAGATGAAATGGTCTCACTGAAAAGCATTTGTCACAATTTTGCTAAGGATTCTTGATTTTGCTCGAAATAGCAAACATTTTCAAATATAAAATTATTATCATGAAGAATTTTTCTAGTAATTGCATATAAAACTGCTGAGTTTTTTTCTAATGGTGCATTTAAAAAAAATTCATTAGCCTTATGAGCACCAGATAAATATTTTTCAATATTAATTCCTATACAAGCAATTGGCAATAAAATTGCAGGTGAATAGCTTAAGAATCTTTCGACTGTATTATCTAAAATTGTTAAATGAGTATATTGATTTATCTTGGCATCATTAAATAATCTTCCATAATTATTGTTTGTAGTTACATAGATATATTTAGAAGCGCCCGCCTTGCCTAACTCTTTATATAATAAGTTTTTTAATTCTCTAAACATAATTAATGTTTCTAAGCATTCGCCAGTTTGTGAGATTACATTAATAGCAAACTTTTTATTTTGAAGTTTAATAAGCAATTTAGCAACTTCTAAACTATTAAAATTTTCATTAACATAAACCAATTTAATTGATTCATTTTCTTTTCTTAAATCAAAATGTAAATCAGCAAGCGCCTTAATTTGATTAATTATTGTTTGAGTTGCTATTATTACTAAATATTCAATACCATCTGATAATAATTCACTTGATATTTCTTGAATTTTTTCATAATCAGATTTCTTTACATTTTTAAAAACATTGAATACACCTAAATAATTTGAATTTTTAACCTTTGAGTTTTTTATATCTAACAAGATTTTTTGAACCGCCTCATAATAACGGTTTGGTGCACTTTCTTTAATTGCTTTTTTTAAATTTATGTCAATAATTTGCTTATTTTCCATAATTAATTCCTTTGTTAGTAAATTCTAATAATCCATCAAATTCTGCATCTTGACTATCTAGTTTAAATTCAAAAGGGTTTCTTAATTCCTTGGGGTGAATTCTTTTAAATGAAACTACTAAATCATTATTTGGCATTATTTCAATTACTTGAACTTCCTTAATATCATTGATTTTAAACATTAATTTTAAATCGCTAATGTAATAATCACTAACTTCATTTATACTCAAAAAGCATTTACATCCTTCTTTTGTTTGCAAAATAATATTATTCTTTCAAACCTTTATTATTTTGGCTTTAAAAATATCTCCTATTTCATAATTCATTTTTGCCTCTATTTTTATATATATTTATAATAAAATTTCTAAATTATATTTTTGTATAAGTTATTAATTAATTTAATAATCATGAATATATAAATTTAAAATTTAATATATATTAATTATTAATGCTTAAATTATATAATATATAAAATTTAAGTTTATACATTTAAAATAAAATGTAAAAATTAATATAATTTGTTTATGAAAAATAGTTTTTATAAATGCTTAAACATTGCAACATTGACAGTTTCCTTGCTTTCAACTAGTCCTATTTTGACGATTAGCTGTGATGTAAAGAAAGATGTTGATTTATTGCATTTTAAAATGATTGTTAATAAGCCTTGAAATGGTTCAAAAAATGATGAATATTTTAATCAACTAATTAAAGATATTAAACAAAATAATAAGAATTTAAAATTTGATTTTAACGTTTCATATATTTTAGACAATGTCGATATTGCCTCTGCTATTAAAGCCAAAAAAGCAAATATGGCTTTTATAACGTCAACTTTATTAAAAAATTCATTGCTAAAAAATGAAGTTATTCCTTTACTTCAAACAAAAACAAGGGCTTTTTATTTTGATAAATTAGATGAAAAATATGTTGATGGTTCTGGCAATGATCCGCTAAGAAAAATAGCAAAAATAGCAAACGATTTATTTAACAAAAAGCCTTATAAATCATGAACAGATAACGAATATCAATGAAATGGAAAAATTTATGAAAAATTTTATGATAAAGAAGGTGTCAATGTAAATTATCAACGCGGGTCAATCATGATTTTTGGAACCGATTCAGAAATTAAATCAATTAAAGAAGCATGAGAAAATAAAGACTGAAATACATTTAGAAATTTTGGAATTGTAATTGGAAAAAAATCAAGTGGAACAAAATACATAGCACAAGAGGATTTATTTAAAAAACATTTCAATTTAGCAAATAATAAATTTTCTTCTTTTGCAAATGATCAAATAAATAATGCTAATAAATACATTACTGAAAAACCTAGAAACATCGGCAAGGGTGCACTGTCAAAATACCACATTGTTTTTGATGAATTTGCATCCTTTGCATATACACACAATTGAAACAAATCAAAAAGCATAAACTATAATTTTTATTGCCCTGATGGTAATAAAAAGATTGAATTATTAACAGTAACTGATCCAATAAAGTTTAATGTCTTAGTTGTTGATAAATCAATACCAAAATCCGCTTATCTTGCTTTTGCAAATTCAATAATTAATTTAGCAAAAGAAAAAAAAGATACATACGGACCAAATGTTGGTTTCAATGAATATGAACTAATTAATGAATCTGACTATTCAAAATATTTTTAATTATTAGCAATTAAATTAAGTATATAATTTTTTTATAAAGAACAGGGGTGCCTTTGGCTGAGAAATACCCTCATTAGCTGATCTAGTTAATACTAGCGTAGTGAGTGTCTTAGTTTTTTGCATCCTCTTTTAGGAGGATTTTTTTATGCTAAAATTTAAAAAATATTTGCTTATAGGGGCAGCGGTATTGCCTGCAATGGTTGGCGCATCAGTTTTATCTTCATCATGCGTTGATAAAATTTCAAACAATACTAAAACTTACGAAAATTTAAAATTTTCAATTAACAAGCCTTGAAGTGAAGCAAGCAATAAAGATAATTTCTTTTATGAAGTTATTAATAAAATTAATGAAAATAAAAAAGGTTATAAAGTCAACAACGCAACGTTACATTATTTAGATGATAATGTTGATTTTATAAAAGAATTACAAAAAGGAACTGCTGATATTGCATGTTTAACACCAACGGTGTACAATCTAGAAAAAGATAAATCAAATATTCATCCATTTTTGCAAACAATGACAAGAGCGTTTGTTTTTGATCAAGATTTTAACGCTCAATATTCAAATGGGTTAGATGACGATCCACTAAGAAAAATAGCTCAATCAGCATTTGATTTATTTAACAAAAAACCTTTTGAAAATTGAGATGATAAAGAATATAAATGAAATGGTTCAATCTATGAATCATTCTATGCAAACAAAGACAAATTAGTTGATTATTATAGAGGATTGGTTGCTATTTGAGGAACACAAGAAGAACTTAAAGCAATAAAAGAAGCTTGGAATAAAAAAGATTGAAAAACATTTAGAAATTTTGGAATAATGCATGGAAGTAGTTCTAGTGGTTCAAAATACTTGCTTCAAGAAGCATTATTTAAAAAACATTTTAATTTAAAAGGAAATGAATTTAAATCATTTTTAGAAGATAAACAAGGAAACGAAACAAAATACACCAAAGGTAAGATTAAAAATATTGGAAAAGGCGCATTTTCTAAATACCATATTGTTTTTGATGACCTTGGATCTTTTGCATATACTCATAGTAAAGGCAGCACAATCTATAAATTGGATGACAAAAATTCAAAAATTGAATTTTTAACAGTTACCGAACCTTTAAAATATAACATTCTTGCTACATCATCAAATATTCCAAAAGAATTAAGGGAAATAATTGCAAATGCAATGGTCGAAGTTTGAAAGGAAGGCAAAGATACATATGGGCCTACTGTTGGATTCAATGGTTACAAGATAATAAATGATCCAATGAAGGAAGTTATATTGCCTTATAAAAATGTATTCAATATAAATTAGTTATGAATAATATTTCTTTTCCAAAATCTTTCTATTTTGAAAAAATAAAATACGATAATTTAACGTTAAGGGGACCAAAAAAAATTATTTGAAGAATTTTAGGTCTCGTTTTTGTTTTAGTCATAATTGCTTTATCATTTTATTTTATTGACTTCTCGTTTGCACCAAACGGAAGAAGGGTTTTTAGTCAAAATATAATTGATTTTTTTAGACCACATTTGATTAGCAAATTAATTCCTGGTCAAAATTTGTTTCTATTATCAATTAAATATTTATGAGAAAGCATTAAGGTTGCTTTTTTAGGAACGATTATTGGGACATGTTTAGCATTATTTACAGCTTTTTTTTCTAACTACAAAATGAATAAATATTGAATAGCAATTCCTATAAAATTCATAATAATAGTTTTAAGATTACTACCAGAATTATTCTTTATTTATTTATTTCAAATTTCAGCCGATAAAACGTTGGCTATAAATTTGATTTTCTCTTGATTTACTTGGCTTTGATTACACGAATATTTTTCTCAAGTAATTGAAAATGCAAATTTTGAGATATTCTATTGACTAACAAAAATAAAACAAAATAGATTCACTGCATTCTTTAAAGAAATTTGGCCTCAAATATCTCCAAAAATTCTTAATTATATATTTTATGCTTTTGAATCAAACATTCGTTGATCTACAATTCTTTCAAAACTTGGTTTTTTAGGAATTGGAATATTGATCTATCCAAATTCAATCAATGTCAATTACTTTCATGAACTTCTAATTCCACTACTAATATTGGCAACATTTTTAATTTTTATTGAATTTTGTAACTTTATAATATCAAATTTGTTGTTTTATTCAAGGACTCAAAAAGTAAATATAAAAAAATATGCCAAAAATTCTACTATTAAAAAAATAATAATTTTTATAACAATTGCAATAGGATTAATAATAACTATCTTTGCATGTTTATCATTAAGGGGAGAAAAATTTTATTCAACCCAAGGAAATTACTATTTCAAGGAATTATTTAATGGTGAATGAAAAAATGTTTTTCTTGGTTGAAAAACAAACAGTAATTTTGGACTTCTATATATGCTTTTGCAACTTGGGGCATTGGTATTTTTAAGCATTTTATTAACATATTTTATATCGTATTTTAAACTGCTATTGCTTTCAAGACAATTATTCAAACGCTCATATTGAATTGAAAAAATTTGAAACACTATTTTAAGATCAATTCCTGTAATTTCATTATTTTTATTAATCTCTAATTTGTTTAATAAATTAGAAGCTGCTTTTGTTATAGCATTTGCAATTCATAGTTCTTCTACTATTTCTAGAATGTTGGAGAGCTCCATTAAAAAAATCTCGGCAAACAAGATAAATGAATTAAAAATCAAAGGATATTCAAAATTCTATATCTTTAGAAATTTCATAATGCCAACAATTAAGCTAGATTTAATTACATTTATGACTTTTGAAATTGAAAAAATAACTAGAAACTTTATTACATATGGTTTATTTTCTGCTTCATTATTAGGATCTGAAACTAATCTAGGAAGATATAAAGAAATCTCTGATATTGCACCATATTTATGAATTGAATTTATAATTATTGGATTAATTAATCTAGGTAATTATTTATATAGATTAAGATTAAATAAAGGCAGTATTAAAAAATAAAATAATACTTATTTAAATATCACTAGATAAATTTATCTCTAGTGATATTTTATTAATTACAAACATTGAAATTTAATTAATTAACTTAGATCTTTAAAGGAAATGTATTATTTAAAAATTATGTAATTTATATAATGTTTGTTATAATAAATATTAGACTTAGGGGTTTAGTATAACGGCAGTACCGCAGTCTCCAAAACTGCTTGTGAGGGTTCGATTCCTTTAACCCCTGCCATATAAAAAAAGTTTGTTAATAGCAAACTTTTTTTAAATTCAATATTTAAAAAATTTGCTTTGTTAGCAAATTTTTAATTTAATTTAATTTTGATATTTTTTAGCAACATATTCTTTAATTTGATCAACGCTATCAAGCTTTTCTCAACTTAAATTAAGGTCGTTTCTACCAAAATGTCCATATGTTGCAGTTTGTAGATATATCGGCTTTAATAAATCTAATTTTTTTATAATTTGTTCAACGCTTAAATCAAATACATGTTCAATAATGTCAGCAATTTGTTCATCATTATATTGTGATGAATTAAATGAATTTATATAAATAGATTGAGGACGTGGCAAGCCTATTGCATAAGACAATTGAATTTCAATCTTGTCTGCTATCTTAGCAGCAACTAAGTTTTTAGCAATATACCTTGCCATATAAGCAGCAGACCTATCTATTTTTGTAGCATCTTTCCCTGAAAATGCTCCTCCACCATGGTGAGCAAAACCGCCATATGTATCAACAATTATTTTTCTTCCTGTAAGTCCAGTATCTCCAACAGGGCCTCCAATTACAAATCTGCCAGTCGGATTAATCAATAAATCATAATCCAAATTGAAATTAAATTCTTTTAACACATAATCAACAATATTTTTCTTTATAAAATCTTTAAATTCTTTTAAATTTGCATTTTCAGCATGTTGAATTGACATTAAAACTTTTTTAACAGTTATTTTATTGCTTTCATCATCATATTCCAATGTTACTTGGCTTTTCATGTCACTTTTTGCATATGGAAATTGATTTTGTCTTCTTAAAAATTCGGCGCGTTTAAGAAGCTCATGTGCTAAAACTATTGGTCAAGGCATATAAAATTCATTTTCATTAGTTGCATATCCAAAAATAATCCCTTGATCACCGGCACCCAATTCATGGCCTTCTTCTTTATTGACACCCAAAGCAATATCGGCACTTTGTGGAGCAATACCATTAATTATATTAAAATCATTTTCATCATATCCTAATGGTTTTAATACCTTTCATGCTTCTTTAATTGTGTCGGAATATGCCTTTGTAGTTATTTGACCGCCTATATAAATGATGTTGTTGTTTGCTAAAACATCGCAAGCTACTCTTGCATATGGATCATTTGTCAACAAACTATCTAATATCGCATCACTAATTTGGTCACATATTTTATCAGGGTGACCTGCCCCTACACTTTCTGATGTTAATAATTTTTTCATTTAAAAAACCTTTCTAACACAGAGAAAGGTTTTTATTATCTTTTTTTGTACATGTTAGATATAAATCCCTTACCATCCACCTTGCTTTATTTAAGTAGGTTGGCATGCCTTAATATGGTTAACTGGCAACTCTCTATGTATTTTTTGTTTTATTATTATACTAAAAGTTTAGTATATTATTAAAAATTAATAATTATAAAATTATAATGTTTTCTTTTGCTGATTCATCCAAGGTTTTTTGATCTCATGCAGATGCTTGAACTTCACCAATATGATGTTTTTCTAATAGAAACATTGCAATTCTACTTTGGCCTAGGCCACCACCAATCGTATATGGCAATTTGCCTTCTATTAAATCACAATGATATGGACTTAATTTTTTAATTTCTTCTTCGGTTTTATTTGATTGTTTAATTAAAGAATCTTTATCAACTCTAATTCCCATACTGCTAATTTCCAATGCTTTTTTATTTATAGAATCATAGACAATTAAATCCCCATTCAACATTCAATCATCATAGTCGAATGCTCTTTTTGAATGTGGAATTCCATCAGGTAGATTGTATCCTATTTGATAAATAAAAACAGAACCATATTCTTTACAAATTGCTTCTTCACGTTCTTCAGGACTTAGGTTTGGATAAAGATTATATAAATGGCTAGAACTAATAAAATAAATATTTTTAGCTAACTTTTGACTCAAAACGGGATAGATTCTATTAATTGATTTTTCAGTATTCTTTAATGCACCATAAATTAACTTAACAATATGTTTTAAATATTTTAAATTTCTATCTTCTTTTTTTATGATCAACTCTCAATCCCATTGGTCTACATATATTGAATGTTTATAATCTAATTTTTCTTGTTTTCTTATAGCATTCATATCCGTATATATTCCTTCATACAATCCAAATTCATATTTCTTTAAAGCATTTCTTTTTCATTTGGCAAGTGAATGAATAATTTGCAATTCATCTTCAACTTCGTCTGCAAAAAAGACAACTGGTTTTTCTCCATTCAAACCGTCGTTTAAACCACTCTTAGTTTTTACAAACAATGGCGCTGAAACCCTGACCAAATTCAAGGTTCTATGAAGTTGATGTGAAAACAGATCTTTTAATTTTTCAATAGCAAATTGGGTATCTTTCAATGATAATTTTGATGATGGCATATTTTATGCTCCTTTTTTAATTAATTTAATAAATTATAAATTAATATAACTCTTAAATATAAATAATTTTTTAATACAATAAAAATCAAGCCTAGTGGCCTGATTTTTATTACTTATATTGCCAAATTTGAAACTATTTCTTTTCAACAGTTAGGCCATATTTAGCACTTACTGATTTAATTAAAGCATCCATTGGCAATGCACTATTACGTAATAAAGCATCAAAGAATTCTTTTACATATTTATGATTATCTTTTTCGACAAATTCTCTTCTTGTTAATCCTAGTTTCTTTTGAACTTTTGTATATAGATCCATCATAACTGCCTTACCTGAGTTATATGAGGTTGCTTGACCAACATATGATAGGTAACGTCTTGATTCTGATGTTATGTCACCAATTCCTAGTGATGAATTCTTCTTCATGAATTCTCTTACTTGGTTGATTGAAGCACCTGCTGGCAAGTCTTCATGTCCTTTTACACCTTTACCATGGTAAGCAGTGTCTAATGCTAAACGCATGTTACGCAATTGAGCTTCGTTTAATGCACCTTGGTATTGCAACAAGTTAGCCAATTTTACAGCATCCATTGCATGCTTTTTATCTTGGTTTTCATATTTATTAACTTGTGCAACTTTATTTCAATATACACCATTGTGTAGGTCTTTAATTTGTTGAATCATGTCATCTGTAACTTCTGCTTCTGTTTTAGCAGTAAAGAAGTTTGTTATACCATGCGCTGTTGAGAAGTCAACTGGCATTGCATATAAATCGCCGTCTTTATTGTCATAATCTGGTGTGCCATAGAATCCGGCTTCAATTCCAAATCATTCCATAAATAGAGCTCAACCTTCAACGTATGAAGTGTAGTGGAATACATTTCCTAATTTATATTTACCAAATTCTCCAACTGATGATAGATACTTTTGAGCATACATTAATTGGTTGTGGTGTCCCATCATTGATTCATGGTTTGCAAATGAAGTAACTGATCATTTTGGTAATGAGAAGTATGGGTCAACATTAAACATGAATTTTTTAATGCCTGAGTTGTAAGCTCCAACACCCACAAATTCTCTTTCATTAAATTTATAGGTTTGAATATCATAATCAGGAACTTCATTTGCAAAGAATTTACGTCCATAATTTTGAGTAGTCTTCTTAAATTGTTCATATGCCTTAAATGCTGACAATCCACCATAGTAGAATTCTTCGTTTGTTATTGAGCCATGTTTTGCATCTTTGTTGCCTTTAGTTAATAATTGATCATAATCAAATTTTGTTAATTCGCCAACGTATTTCTTTAAATTAGGGTCTGTTTTAAGACCATTTTTTATAGTATCTGTTCAGTAGGCACTACCTTCACGTCCAAAGAAGAATTCTTCGTCATTTAATCATTTAGTAAATGAAGGAATATCAATGTTTCCTTTTTCATCACGAATTACAACAGTTTCTTCTTTTATTTCTTCTGGTCCAATACCATCAGCATCGTATTTAATTTTAGCTTCTCATTTTTTGCCTTTTCCAACAATTAAATCAGCTGCTTGGTTAGCAATTTTCTTTGAGTTTTCAGCAGATTTCTTTGTTGTTTCATAACCAATATTGTTTACTTGATCATCTGTTAAATCAGAAGTTGTAGCCATCTTTAGAATTTGACGATACATCGATTGACCATTTACGGTAGCATCTCCTTCAGCAAATCCCAAACCAACATTTCTTTGATTTAAGTCTTTTTCAGTTAATCCTAAACCATATAATTTAACAATTTTTCCGCCTTTATCTCTAGCATCAATTGATAATTCTTTTTCGTTTGTTTTATTTTCTTTATAAAGTTTTAATTCTTTAATATCTTCACCCAATCCATGAGTTGCAGCATTGTAATAAGTAGTAGCATAATATTTTAGAAATTCTTTTCAATTTCCTTCCAATGATGATGATTCAATTAAATCTGCAACAGATATTTCGTTTTTAGATTTATCTTCAACAAACTTAGTTAATTCTTCTTGGAATAGTCTGCCAACAAAAGCATTAACTACCATTTTTGAATAAACTTTTGACATAACTATTTTATGTTGAACTGCTTCTTTAAAGTTTGCTAATAATTGTTTATATGGTAATTGACCATACTTTCCGTATTGATTGCTGAATGCATTCATTGGATATACAGAAGCATTTCCATAAAAGAAAGTAGGATTTAACAATCTTAGTTGGTTGTCATGGTGTCCTTTAGCAATTTCTCAATCGTATTTTATTGCATATTTTCATGCTAAAGAATCACTTGTTAGTGAAGCATCATTTATTTTTGAAATTTTTTCAATATATTTACCATAAATTTCAGAAATTTTCTTTGATTGTTCAGCAGTAGGAGCACTTATATCATCTAATTTTGCGTTTTCCAATTCATTTTCAAGGTCGTCTTTTAGTTCTAAAATGTGTCTTGCCGCTGCTTCTTGACTGGCTGGTGAATTATCATTTTCTTTTGAAAGTAATTTGCTTAATTCATCACTCTTTTTAACACTGTCTGCATATTCCTTATTCAATGCTTCTAATTCTTTTTTATTTGCATCAATTTGTTCAGAAACCTTATTTTTATTAGTTTCCAATTCTGCAATCTTCAAATCTAATTTTGACTTTTCAGAAGTTTTTTTAATAATTTCATCTTTTTTAGTTTTTAATTCTTTTTGCAATTTATTATTTTTTTCTTCAGCGGTTTTTATTTTTAAATTTTGGGCATCTATATCTTTGTATTTTGCTTCTATTTCTGCGTTTTTAGAACCGTCAGTATTTGCTTTGTTTAGGGCTTTTAAGTCCTCATTCAATTTATCTAATTGAGATTTTAGACCGCCTAAATCTGTTTCGTTTTTTGTTAATTCTTGTTCTAATTGTTTTGCTTTTTCTGAACTGGTTTTTATTTTCCCTTCTAGGCTTCTTGATTGCATTCTAGAAGGTACCAATTCTTTTGATAAGTTTTCTAATTGATCTTTAAGATCATCATTTTTTTGTTCTAGTTCCTTATATTTGTTAGGAATTTCAACAATAGCCTTGTTTGCAGCATTTAATTCTGTTTGATATTTAGCTGTTCTTTCTTGTTGAAATTTATTTAAATCTTCTTGCAATTTTTTAACTAATTTTTCTTTTTCTTCAATTGTTTTTGAATTATTTTTTGTTTCTTGCAATTGCTTCATAAGATCATTAATTTGTGCTTGATCTTTTTGAGAATTTGCTTTTGCTTCATTTAATTGCTTTTCTAAAGATTCAATTTTTTTGTTTAATTCATCATTATTTGTATGTGTACATGATGCTGCCATAGGCATTAAGGCTGCTACGCCAAAAATTGAACCTAATGAAATTAGAATTCTAGTTTGTTTTTTCATTTTTCCCTTCTTTAACTTTTAACTTATATTGTTCTTTTTTACAACAAAGTTTTTATCTTTATATAGTCATAATTTTAGAAAAAAAAAAAAAAAAAACGCTTATTTTGTAATTTTTTAGCATATAAAATACAAAAAAAATTAACATTTTCTTATAGTTTTAAGTACAAAAAAGCATAACAAAAGCACAATAAAAATTTTTTGTTAACTTTAAGTAGTGGAATTTATGCTTTATTTTTGTAAACTATATATTTTTTAAAAAAGTCATAGGAGATAAAAGCTAAAAATGTTGTTACTTTTTTTAAAAAATCTAACAAAATAAAAAATTTTTAAAAATTTTGAAACATATTTAAAAATAGTATATAATAATACACGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCCTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTTTTTATATCTTTTTTTAATAAAAAATAAGGCTTTTAAACCTTATTTATATATAGATTAATATAAGTTGCGATTAACAATTATTATTTTTAAAAATTTTTTTACCTAATTTAATATACTTATCAACTTTTTGATTCAAAACCTCAAATGCTAAATTATAAATATCTTCATTATAAATATCAACACCAGCATCTTTTAAAATATTTGCAGGTCAATCGATGTCTCCGGCACTTAAAAATTTATTTATATAGTTTTTTAATGCTTCTTGGCCTTCTTGTTTGTATTTTTGGAAAAATACATTTGCTACGATATATCCTAATGCATATTTATATACATAGAAATAATAATAGAAGTGAGGAACCATTACGCTATAAACATTTCTAGGATCGCCTAGTTTTATTGATTTTGATGTAGTTGAGTATTTCTTTTCATTTTCAACATATAATTTTTCTATTTCTTCATATGTATTTAATGGTTTATTTTCATCTATTGCATTATATAGATCAAATTCAAAATTAGATCACATCGTTTGGCGCAAAACTGTGCCAATAAAATCATTAATGCTTGAATCAAGTAAATAAAATTGTTGTTGAATATTTTTTGATTTGCTTATTAAATAATCATTTAGTAATAATTCATTAAATATCGAAGCAATTTCTGCTAAAAATATTGGATAAGAGCTTCTAAGTGGAGATTGGCATTTATTAGAAAAATATGAATGCATTGAATGTCCCATTTCGTGGCATAATGTATTGACTGATTCCAATGTTCCATCAAAATTCATCAATATATAAATTTTTTTCATTCCTGCTGCCCCACCTATAGAATAAGCGCCGCTTCGTTTAGATGGGACATTAATATAATCAATTCAACGTTCATCAATAGCTTTTTTAACAACTTCTGGATATTCATAAGGCATTATTGAAATAACATCTAATAAAATCTTTTGTGCTTCTTCTATTGTATATGAAGATTTAACATTAACTAAATCTAAAAAGTAATCTCATTTTTCTAATTTTTTATTGAATTTAGCTTTAAAAAATTGTTTTTTGGCATTGTTATATTTTTTAAATATATACATATGTTTTTGAACTGCATCATAAATAATGTTCAATAACTTTTTATCAACATGATCTTGCAACAAAATAGATTCAAGAGATGAATTATATTTTCTATATAAAGCATTAACTGATATTTCTTTAATATGTTGATATAGCAATCTCGCTAATGATTGTTTGTTTTTTAAATATCCATTTAAATAATTAAAATAAACATCTTTTCTAACTTGTTCATCTTTATTTTTTAATAAAGCTATTCTATTTCCTTCAGTTATTTTTATTTTTTTATTTTTAATTAAAACATTGCCAAAATCAACTTCACTATCGCTTATTATTGAAAAAATATCTTCAGGTACAGGTGAACCGCTTGCAGTATCATTTAAATAATTTTCAACATCATCTGACAATTTATGATCAAGTTCTTGCAAAGTTGCTTCTAGGTCTTTTTTTACTTCAATTAGTCTGTCGTCATTAATTCATGTTTTGATTTTGTCTTTATGTTGAAAAATTCTGTTGGCTTCTGAACCTAACTTTTTAGCATATTCTTGGGATTTTGTTTCAAATAATGAAATTGTTTTGTTTATTGTGGGATCAACAATATTTATATTTAATTTATTATCCAAATAATTAGAAATTCTATTGCCTAAAATTAGGAATTTTTTTGAAAGTTCAATATATTCAACATATTGGTCTAATGAATTGTATTTGCTATCTTTTATTTCAATTATTTTTGAATACAAATCAAAATATTCATCTTCTAATTGTTGATAGGTTTTATTACCTAATATGTCTTCAATATCTCATTTATATTTTTCTTCAACGTCACTATATGAATTGTATTTTTTTAACATTATTCCCCTTTATTAATATAGATTAAACTTTTTATATCGTATGGCAATCTTTCATGAGAATTAAAGCCATCTAATTCCATTAAAACAATTATTCTACTGATAATTACACCTTGTTCAGTTAATAATTTAGTAATTGCTTCCAATGTACCCCCGGTTGCTAAAACATCGTCAACTATGACGGCTTTTTGACCTGGTTTAACTAAACCTTTTTGAATTTCTAAAATATTTCTACCATATTCAAGTCCATAATCGCAGCTTATAACTTCACCAGGCAATTTGCCTTTTTTTCTTACCATAATAAATGGTTTTGATAATTTAGCAGCTACTGGTGTTCCGAATAAAAATCCTCTTGCATCAGGACCCACAATAATATCTGCACCTTCTGCTAATTTAGCCATTTCATCAATTGTATAATGTAGCGCTTGTCCATTAGCTAATAAAAGCGAAATATCTTTAAATTTGACACCTTTTTGTGGAAAGTCATCAACAGTTCTTATATACTTTTTTAAATCCATAAATTTCTCCTAACAATAAATTGATAAATAACGAATAAATTTTAATATTATTTACAATTTTATTTTATAAAAAAATGATTTTATTTCAAACAATTGAATAAAAAACTAGTTAAAATAAATATATTAATTTGCTAAATTTTTTTTATTTATTGATAAAATAAAATTAACTTAAATTCTTTTATTTTAAATTTTAATAATTACTCGGAGGATAGAATGAATAAAATTAATTTTTTTGCATTAGGTGGTTTGGATGAAAACGGCAAAAATTGTTATGTTTTGGAAATAAATAACAAACTATTTGTGATTAACTCAGGAACTAAAATTCCTATTAACTCGCATAACGGTATTGATACATTAATTTGCAATTACGAATTTTTAGAAAAACGTCAAAAAGATATAGAAGGATTATTTTTGACTGATGTTAGAAATGAAACGTTTTCTGCTATTCCTTGATTATTAATGAAAATTAAAAACCTAAAAATATATACTAGTGCTTTTAACAAAATTGTTTTATTAGATAGAATTTCTAAATATAATATAGGTTCGAATACATTTGAAGTTAAAACAATAAATAAAAAAACAGCATTTGGCGGAGTAAATGTAACTCCTTTTGATCTAGCCGGAGGATTGCCTGGACAACTTGGGTTCAATTTCGAAACCCCAATTAGCAATATCTTGTTTATGACAAATTTTGTTGATGGTGATTTGGGTCCTTATGGTAAAACCGATTTGCAACAAATTCAACAAATAATTAATAACAAAAAGGAATTACAGCTTTTAATAATGGATAGTTCAAGAAGCGCTTTTCCTGGACGTTCAATTGAAAAACTTTGAGTTTCAAGAAAAATTGAATACAAATTTAAAGAAACATCAGATAACCAAAGAATTATTGTTGGTTTATATGATGAAGATATGATAACTGCACATGAAATTTTAATACTTGCAAAACGTTATAACAGACCGGTTATAACTTATGGTTTAGCTTATTCTCAATTAATAAAACTTGTTGAAAAGGTAAATCCAAATTCTCAATTTCCAGAATTCGTTGAATATCAAACTGCAAATAATGTAAAAAATGCTGTTATTCTTGTAACGGGTGCAATAGATAGACTTTACACAAGATTTGCAAGAATTTCTAGCGATAATGATGTTTATTTAAAATTAAAAAATGATGATGCAGTCATTATAATTGCTCCACCAGTTAACGGATTGGAAGTAAATTACGCAATAACACTAGATGATATTGCTAGACATACTGCTAATTTAATTGAACTAGGCAATGATCAATATTATTCATGCAATCCTGCTAAATTAGATATTTTTAATATTATTAAAGCATTAAAACCTAAATTCTTTATACCAATTCAAGGTCTATACCGTTACCAAGTTGTAGCAACTCAAGTTGCTAGAAGCGCAGGTATGAACATTAGTAACTGCTTAGTGTTGCAAAACGGAAAGGTGGCTGAATTCAACGATACAAATTTAATTTCTCAAAAACATGGAATTAGAAATGTAGGTGATGTTATTATTGATGGCTATGGTATTGGCGATATTTCTCATGAAGTTATTAATGAAAGAGAAAACTTAGCAAGAGATGGTGTAATTGCTCTTAGCTCATTAATAGATTATAAAAAGAAGAAACCAATCAATGAACTTCAAATTTCTTCATATGGAATAATTACGAAAGATAATAAAGAAGTTATTCACAAAATAATTAATGATTTATTCAAAAAAGAATTTGAAAATAAACAAACAAAAGAAATAAATTTAAAGGAAATTCAAGAAAAATTAAGAAAATCAATTAAAAGAAAAATCGCCAAAACTATCGACAAAGAGCCAATGATTGTAATAACTTTATATGAAATTTAACTTTTAAAAATTATAATTATAGTGATAACTAGGAATATTTATGAAAGTGGCAAAGGAAAAACAAACTAAAATTAAGAACCAAAAACAATACTTTTTTTCAAGAGACAGGGTGCTTTTATGAACAATAATACTTTTAGTAACATTGTTTTTTTTAGTTATATCTTTCCTTAATGTTCCTTGATTAACAACATTGCATTCATATTCATTTAATGTATTGTTTGGAATGTTCTCAATATTATTTTATTTATTAATTATTCTACTTTCAATATGAAAAATCTTTAATTTAAAATCAACGTATAAATATTGAATATTCAATTTCAACCTTGGAAGATTAATATTCTTTTTTATTAATATTATAATTTTGGGTTCTGTTATTTATTACACCATTCATCAAGTTGAACATAATTCAAAGAATTTTTTTACTAAATATTTTAGCAGTTGATTTGTTGATTTTAGTAATAGCAAAAACTATTTACTACCTCAAAAATACACCCCCGGAGTGATTGGAACATTTATTAATTCAATATTAAATATCCCTGGAAAAAAACTTGGTTTTTCAATAGCTTACATAATAAGTATTGTTCTTTTAAGCTTATCTGTCATTTCATTTTTTATTAGTAACTCTTTCTTTAAAATGATATTCTCTCCAAAAAGCAAAAAGAACAAGGAAAAACTTCAAAATCAAAGCGATTTAAAAATAAAGGAAGAGGATATTGAAATAATTGATAACAAAGAAGTTGTAAATAGCAATGTTCAAATTTTAGAGACTAATTCCGACTCAAATAAAAATATAGACTCAGCACAAAATTCAAACCAAAATAACAATCTTGATTTAGATCCTTTTGATGACGAAAAAGAAATTGAAGAAGATTCAAGGCAAAAAGACAACAATGAGCTAGAATCAAAAGAAGCTATTGACTCACCAAGCAATGAAAATTCTATAGATCCTACACTACAAGAACCAGATAATTCAAATAAAAATAAAGAGGTTAAAAAAGAAGATAAGATCTATAAAATCATTGAAGACGAAGAAAATTTGTTCTAATTAAAGAATTCATTTTTTGCAAACATTTATTTATAAGAACAAATCTCAATAACAAAATAATTTAAGCGTTTTAGCTATAAACAAAGAGCATTTAAATTGCTCTTGTTTTTTTATATACAAATTATAAATTTACATAAATATTTTTATATATAATTTTTAAGTAAAAAATTTATGATTTAGTTTGCTAAAAAAAGCAAAGTATAACTGATAGACTTCATATTTAACAAATAATAGTTTATTAATTTAAAATAGTTTTTTATTTTTTAGATGTATTGGTTCAATATATCTTAGTTAACTTTTAAATATACACACCCAAGCACAATAAATGATATAGAAAGGTGCTAGTAATGAAAAACAAAAAAAGTAAAAAAATTGTGGTTGCACTTTCATTAACATTGATTGCATCCGCCATCACAATACCCGTTGTAATTTCATCTTTAATTATAAATAAGAATAATTGGGCTAACAAATACTATAGCCAAAGATCAGAGGCTAGTCATCTAGTTAATTTAATAGAAAAAAATAAGCATTTTTATTATTTTGCCTTAGAGGATCAAAATAATTTAAATAAGTTATTGAATAAAATTAATTTAACTAGCAAAAATAGTTCTGCCGATTTTAAAAACTCATATAATTTGCTTAACAAGACAAATCTTGATATCAACAAAAAGATAAATATTAACAAAAAAGAATTTGACAAAACTTATGCTGATATTTTGCTTTTAAGCCCACAGTTGCAAGATTTATTCAATAAAATTATTGATTTGAAATTGAACAACAATATAAAAGAAGATGTTGATGGACTATTACAAAGAGTTGAAAAAGATCTAAATAAATGTAGAAAAATAGATGAAAACATTAATCTTATTGATATTAAAGAAATATACGCACATAGTTCAAAAACAATCATTGATGTTTCAAATCAATTGAATTCATATTATAAATTAATTAGCAATAAAGATAAAACATATAAAGGCATTGTCTCAGAAATAAAAGATCTTAAAAAAGATTATTTCAACAAAATTGAAGATGGAGAAGAATATTACAAATTATATGGCAATGAAATAAACAATCTTAAAAAGAATTTTAAAAATTTTCAATCAGTCGAAGATTACAACAATTATATAAATTACGCCAATTCAAAAATAAAAGAAATTAAAGAAGAAATTGTTGAACACAGAAAGCTAAGCCAAAAGATAAAAAAAGATGGAAATGATATTCTAAATAATGATTATGATAAAAGTGCTTTAATAAGAAATTCATACTTCCAAGAACCGAAATCATTTTTATTAATGTGAAATACCTTCACTAAATTAGATGCGTTAATAAAGGCAAAATTTATTAATGAACGTCCTAAACAAATAAAAGAATTCCTAAAGCAATATGAAGAAAGGCAAGAATACTTTTATAGAATTAATGAAAACGTTCCAAACCTTATTTACACATGAGTAATTTATAATAATAAAATTAGAATTATAAAAGAATTCTATTTTAAAGATAAAAAAGAAAACGAAGAATTTAATTCATTTTATTACAAGGATAAAGATATAGACAATCAATTAAATTCGCTTCTTAATGAAGAACGAGAATTTAAAAATAGGCAAGAAAGACTAGTAAGATTGTTTTATGACAAGGTTATTCAAGACCCAAAAACTAACGAAATTGATGAAGAAAAATTAATGAAATTAGATGACATCCATTTTGACCCAGAACAAGACATTAGATATTTACACTACCATTTTGGAAGTCTCATAACTCAAAAGTTTAATGAAATAATTAAGCATTATAACGAAAAAGCAAAAGCCTTATTAGAAAAAATAAAAAACAATAATAGTTACGAAACACAAAAAAATAGAATAGAAAAAATACTTGCTAAAAATATTACTTATGTAAATAATTTTAGAGGCGATTTCAATGATTACGAAAAATTCTATTGGTGAACATATAAATTTCAAAAAGACAGCAATGGTGATATAATCACTTCATCAAACAGCGAAATCCCTGATTTTTATAAAAATGAAATTGAAGTAGCCCAATACCTACATGAACTTGATGAAATACTTGATAATTTAAAAAACATTTAAATATTTGTTTCTTTAATAAAATGGCATTATATGTGCCGTTTTATTTTTTAATAATTAAGTCAAAAAATAAATTAGATTTTTATAATGCTTAAAATAAGTTACTTTTTAATTAAAACTTAATTTGCTTTTGCAATAAAAAAACTAAAAATAATAAAAAAATTTTACATATTTGTTAAAATTAAAATGTTTTTTTACAACGAATTAAAAAAATAATACTTTTAGAAAAAATTAAGGAGAATATTATGAAAATGAGAAAGAAATTAATTGCTTTATCTGTGGCTTTTGGACTTTTAGCGCCTATTTCAGGAACTATTATGATTTCGTGCAAAAACGAAAAAAGTAATGTTGAATATCAAAAAGAACTGCAAAATAAATGAACAGAACTTACGAATCTTTTAAATTCAAATTCATTTAAAGAAGTTGAATCTAAGTTAGATCTTTCAAAATTAAACGCTCTTAAAGAAGAATATAAAACAATAAACAGCAAAAGCAATGTTTCAGAATTGAAAAATTTTATTGCAAAAATTAATGAAACTACACAAGATATAAATGCACAAATTTCAAAATTAAAAAACGAAAACGGAAATACAAGCCCTGAAAACAATAGCCAAAATGATTTATTAAAAAAATTAAAAACAGAATTGGAAACGGCAACTAAAAAACTTAACATGCTACTTTCGGCAAATGAAAGTAAAAACATCTCAACAAAAGAAGAAGCTAAAAATATATTAAACGCCGCAAATAAAGCGTTAGAAAAATCAAATGAAAAAGAATATAAAAATCAAATTCAAAAAGTAAATGAAACTATCAATAAAATTGAAGAAGAAATATCAAATTTAGAAAAAGGCCCAAAATCAAAAGAATTTATTAAATTAAGAGAATTAAGAGAAAAGCTAATAAATTACTTAAATAGTAATTTGTCCGACTTTGCTTATAAAGATTTAAGAGCTGAAATTAAAGAATTAATTCAAAAACAAGAAAGCGTTTCAATTGTTTCAAATGAAAAAGAAATAAAGTCAGCAATTATGCAAATAAATGATATTTTTTGAATTGCCAACATCAAAAAATCAATACTAGATAAACAATTTCTTTATTTAGAAAATAAACCTAAATCTGAATTAGACGTAAAATTTTTAGCAGCGCATGACGAATTAAGCCAAGCATTGAATACCTCAATTTTTGAAAACATTGAAACAACAAATGAAAGAAATCTATTAAACAAATATATTAAAACAAATTTAACCAAAATTTCTGATGTAGAAAAACAAAATTATATTGAGGAAGTTAAAAAAACTATAAACTCTTTGTTAAACAAATATAATAATAATAATAATAATATAAATAACAATAATGATCAAATTTATGAATTAAATCGAAACATAGAACAATTAAAAAAATTAAACCAAATAGAAAATAATTATGTTATTGACAAAGTCAAACTACTAAATCTATTAAATAAAGCAAATACTATAAAAAATGAAGGTTCATTTGCTAAAAAAGCAATTTTACTAGAATCGTTAAGAGACAGCGTTTCAGAATTTCTTGAATTTTATTTACAATTAAGCCTTTATCTAAGAATCTTTTTTGATAAAAATAACGACATTATTAAATTATTACCAGCAGATTCACAAACAAAAATTGATGATTATCTAGACAAAATTAAAAAAATTACAGAAAAGAATAGTCAAGCCAAATGAAGCGCGCCAACATTTGACGAATACATTTCAGCCTTTAAAGCACAAGAAGAAATTTTTAAAATTATATCTAGTGATCTAAAATCAGCAGATGTTAACATATTAGAAAAATTGACAAATAATTTAAAAGAAATTAAAAAACAAAAGAAAAATGATTATGACACGATTGTTACCTCTTATTTAAAAGAAAAAACAGAAGAAACAATAACAAAAAATGAATCAATAATTGCTAAAAAATCTAATATTGAAAGTCAAAATATTATTGAAACGTTTGTTTTATTGCAAAAATTAGAACTTGAATTTGCTTACATTAATTGAACTGAATTAAATTTGATTACTAAAATTAAAAATAAATTAGAACTGTTAATAAAAATCAATAAAATAGATTCAAATGAAGAAGCAAAAATATTAAAACCTTGAACAGATTCGCTTAATAGTTCAAAAAAACCAGAAATTTTTGAATTAGAAAACATCTTGAAAGAATTAAGCAAATCTTTAAAGGAACTAATTAACAAATAAAGGAAGCGTAGAAAAATGTGGGGTTAATTCCAAATAAGGAAAAAAAGCAAGATCATAAAGGTCTTGCTTTTTTTCTATCATTCATAAAAAAAATAAGTTTAAAAAAACGAAGAGCACAACACAAGCACAGCTCTTCATACATCTACATAAATTATACCACGTGCTCTTCGTTTTTTTTAAAAAACTTAAAAATTTTTGAAAGGAATTTTATGGGTGCTTATAGTGAATTTATGGTAATGCAGCAAGAGGCTAAATTAAACAACGATTATAGCCTTGTGGATGCATATGAAAGAGATTTAAATTGGAGGAGAAATTATAGTAAAAAAATATATGACAAATTAAGCGACATAGCTATAAGCAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATGGTTATTAAATCCTTTAAAAATAAAAAAATCAAAATCATTGATCTAATAAATTTAGGGTTTAAAAAAACTTCTATAAAATCAGCTATAAAAAGACTTATTGAAATAAAAGTTTTAGATGGTGAGTTTTATAAAAAATATAAACTTCTAAAATTAAAAAAAATAGTACTAAAAAACCCTAAGGAAAATTACTGGATTTTAAATGGCTATGATGCCTTAAAAATCTTTCTTCTTAATGGTTTAAGTAATGCTATTTTATATGCAATAAATAGGCATAAATCAAAGCAACTAAATGCTAAAAAATTGCATTGTAAAATAAACTCAAGAAAAGTGATTTTACAAAATGCATATTATGCAAAATTTAACTGATCAAATAGCAAAATATATTTAATGAACAAAGCCATAGCCAATTATTTTGGAGTTGAATATTTGCAAATGTTTGGTATATTGCGTAGTCAAGAAAAAAAAGTTACAACATTAAAATTAGGAAATTTAAACATCAAATGTAATGTTTATAAATTTTCTACACAACGTTATTTGTTAATGCAAATTTAAAAGTTTTAAAGGTTTTTATAAGCAGTAATAAATGGCTCCCAGCATTGGGCTAATGTTTCTGCATTGTAGGTTCAAATCCTACGCGAGCCACCAAATAATATTTACATTTTAAATTACTACTTTTAAAAACGTTTAAATTTAAGTTTTGATAGTTACTGGCACTAAGTGTCTATTTTAGTCATAAATAAATTAGTCAAATTACTAATACAAATTAATAAGAGCGAATATGCGGTCAAAATTTTAAAGATAAATATATAGCAGTAATAAATATAAATAATTACTAATGTTTTAGAAGGGAGAATAAATGGATAAAGTATGAACTGAATTTAATTTTATTACTCCTAACACAATGTATAAAACCAAACTTGGTAATACTCACGATTTTAAAAGTTGAGTTGATTCTGGACTTGTGGTAGATTACATTACAAGACCAGATAAATGCGTTGTATTTAAAGAAGATGAAAATAATTATGATGCTATGAAACTACTTCTTACTAAGGAAGATAAAATAAATTGATATAGACAACATTTAAAAATAAATCAAAATTCTAAACAATCAGGTCTTTATAACTTATTAGATAATGATCCAAAGGATGTAAATTTAGAAAATGTCAAAAACGATTTAAAAAATTTAAAAGGTAATTATTGAGAATTAATCGTTAATCCTGGTGATTTAGGCTTAGATAATAATGTTTTATGTAAGGATAAATGACATGAAGTTTTAAAACCTAATTTTGAAAGATTTTTAAAAGCTAATGCCTTTGATTTAGAAAATGTTAATGTTTTTTATTCTATACATGGAAATACTGATTATCCTCATGTTCATATGTTTTGATATGAAAAGGAGCATAAACACAAAAAAGCCAAACTTAATTTAAATTCAATTAATGTTTTTGGCTATAAAGTGGGTCTATCTATTAAATACGATGAGGATTATCAAAAAATAAATGCACTTACAAAAGACATTTGAAATACAAGAAAACAAATTGTAGGAATAATTGCCAATAATTTTGCTAATAGTAATGAAATTATTTCTAAAAGTAATTTGTGCCAAGAATTCATTAAATCTTCAAAAAGTATTTTAGATGAAGTTTTAAACAAAAAAAATATTTCATATTCTCGTATTTCTGATGAAAATAAGAAAAATATTGAAACAATAAAAAAATTCTTATTAAATTATGACAATGATTATTCAAAGCTCTTTAATTCTTATCAAAAACAAATTAATGATTTAAATAATTTAAACATCGAAGATAAATTTCTAAAACAAAGAATTTCAAAAATTATAAATAATGAAATGGATGATTTTCAAAAACAAGTTGGTAATAAGATAATCAAAACCTTACTAAATGCCTATAAGCAAAATAATAATGCATTTACTAGCCGAAAGAATTTCGCTTCATTTTTCAATGAATTTCTAAAAATATTTAGCCTAAATAGATCGGAATGAGCCCTTAGAAATCTAGCGGAAAGAGAATTTTTGGCTAAAGATTTAAAAGAACTTGAACTAGTAGAAGAGTTTTATCGTAGCAAACTTGTCCATTAGGAGTTTTATGGAAGAACAAGAAAAATTATCAAAATCAAAAATTATAGGAAAGACACTTCTTTCAATAATTGTTAGTACATTTTTGTTTTTTGTTCTAACTTTTATTATTAATGTTTTTATGGGCCAGAATAAAAATCCTGGACATTTTAAACTTTTTTGAAACTCTACTGTTACTTTATTAAAAACCAAACCTGATATTTTTGTTTATATAGGTCTAGGAATTTCTATATTAGTCTTCTGTTTCATTTTTTGAAGGCAAATAAAATCTCTTGGCAAAAAGATATCAAAATACTTATTTGGTGCAAAGGCTAGCCAAAATTGATTATGAAATTATCAATTAGGAGAAGGCTCTAAAACAAAATACAATAAATTATTTAGAAAAATGATTAATGCAGAAACAGAACCAAATTGATTATTTACTTTTGATAAAACTCAAAAATATTGATATGTAAATGATAAAGATGATAACTCTATTGTAAATGGACCGCCAGGATCAGGGAAGACTCATAGAATAATTTTGCCAAATATTGAATTTATGGCACAACTTGATTATTCTAAAAAACCAAATTTAATAATTAGCGATCCAAAGAAAGAAATTATCAAATTTACAGGAGGCTTATTAACTGAAAACGATTACAAAATTTATGTAATTGATTTCTCTGACCCTAAATTATCTCTAAAATGAAACCCTCTTTCATATGCATGAGATTTAGCGGTTGATGGTGAAAAATTCAAGAATTTGACTTTCGAAGAAAGAATTAATAAATCGTTAGAAGTTATAAATAATGTTATTGAAAGTTTAAATTGAGGTAAGGCAGTTAATGGCAATATGTGGATTAGCCAAGCTAAAAAAACATTAATTGTTATTACTAATTTCTTATTGCTATTAGGTATTGATGATAAAAATATTATTCATAAAAATAATTTTAATTTCGAAACAATAGCTTCATTATTAAATACTTCTCACTGAGAAAACGATAAAACTATCTCTGCTTGAATCTTATATGCTAAAAAAAGAGCGCTTGATGACAATAATTATTATTGAAAAAAAATTTATAACAATATAGAAGATTTGTCAAAAACTCCTAAAGAAACATTTGGTGGAATATTAGCCGAAGCACAAAGTGTTTTAAATGTATTCAATAATGATGAAAATTTAAAGATGGTTTTAAGAGACACAGAAAGTTTCAATTTAGTTGATATAGTGTCCGATCCTAAACCTTTTGCTATATTTATACATTATCCTGACCATAAACCTACATCTCACTTTTTAGTAAGTATGTTGGTAGAACAAATTTATCAATCGTTAATTGAACAAGCAAACAAAAATGATAACCTAAAATTAGACAGAAAGGCTTTCTTTCTATTGGATGAATTCGGAAATTTACCTGTTGTTGTAAATTTTGATAATAAACTTACAATTTGTCGTTCAAGAAATATTTTCTTCATGCTGGTAGTACAAGATTACAATCAAATTAAGAAATATAACATATATGGAGAAGGAACAGCCCAAGTAATTAAAAACTCTTGCCAAGTAGTATATTTTTTAGCCTCTAATGATGAGCAAACATTAAACGATATTTCAAAAGCGCTCGGAACAAAAGAGGTTAAATCAAAAAGTTATACAACAAGTGATAACCATTCAAGTTCTACTGAAAGTGAAAAATCCAAACCTGTCATGGAAGTAGCGGAACTTAAATCTAAAAAACCTGATGATTTAATAATTATAAAAAATGGTTATAAACCTTTATTAATTAAAACCTTATTGGCTTATAAATATTTCCCAAATGATAATTATGTTTATACCAGAAACGAAAGTAAGCTACTAACTAATGCGGATTTTGAATTATTTGATATTAGAAGTTTGGAAAAACTTATTAAATTTGATAATTCTAAACAAAAAAAACAAAATAATAAAAACCCAAAAGTTTTAGATGATAACAATTTAACTTATTCAGAAGATGTTGAAGATCCTGCGGATTTATCCCAAGAACAAAAAATTCAAAGAATGAAGATGGTTTTTGGAAAATTAGATCTTTGAAACAATTATATAAATCTAAAAAATGATTATAAACGTACATTAATACCTCATAAAAAAAATCAAATTAAAGAACAAATAGAAGAAACATTAAGTTTAATGTGAGAGCAATATGTTACCAATGATCTCGAAAGCGATTTGCTTAATATTCTTTGATCTGAGGCAAAAGAGCGCAAAATACCAAATTATCACGATTTGAATGTTGATATATTGCTTTACTTGTTGCATCCTACCGAAGACATCTCCAAGTTAATTGAAAAATCTAATTAAGGAATTTTACTATGAATATAAATAAAATAAAAGTTGTTGAATTATTTGGCGGAATAGGCGCTATTAGAAAAGCTCTAATTAGAACCATCAAACCTCCTAATTTTGAAATTATTGATTATGTCGAAAGTGATAAAAAAACAGTAAATGCTTATAATGCAATATATAACGATTTTTACAAACCTTTAGATATAAGAGATTATTCACTTCCTAATAATGTTAATGTTGATTTATTGTTTCACGGATCTCCTTGTCAAGATTTTTCGTGTGCAGGTAAAAATATCGGAGGAAGTAAAGAAAGTAAAAGTCGAAGTAGTTTATTGTGGGAAACAATTAGAATCATTAAAGAAGCTCAAATAAAACCCAAAATTGTTATCTGAGAAAATGTCAAAAATGTATTAAGCAAAAAACATAAACCTATATTTGATGCATATATTAAGGCATTAAATGATCTAGGATATGTTAGCCATTATAAAATAATTAATTCATTAGATTGTGGAATTCCCCAAAGTAGAAATAGAGTTTTTACTGTTTCTATTAATAAAAACTATTTAAAACAATTCAATAATTTAGATACATTAAATTGTCTAAACAATAAATATAAAAATATAAATGATTTTTTACAGCCAAATGTAATAAATCCAAAATACTTTATTAAAGCAAATTGCCCCTCAATGATTAATGCCATAGCAAACCATAAAATAAAAATAATAGAGAATAAAGTCTTTACTATAACGACTAAACAAATGCGTTGAAATAATCAAGGGGTTGTTAAAGTAGAAATTAGCAAAGTTCCTAATAATAGCAATTTAGCAAAAATGATTATAGATAATAAACCATATTATTTAAGGGTTATTACACCATTAGAAGCAATGCTATTAATGGGCTATGATCAGCAAGATTATGAAAGAATTAAGAATTTCTGTGATAGTCAAATTTATAAGTTTGCTGGAAATTCTATTGTTGTTAATGTATTGCAAGAAATAATTAAACACATTTTTAGACACAAACAATAAGGAGAAACATGACAAAAGATAAATATTGTGCTTTTTGTGGAAAAGAACTAGTAGGAATGTATGTTAAGAATTTTTGATCTGATTTTTGTTCAAGAGAATGTAGAGCTAAATCTGAAAAATTGCAAGAAGATTTAAATTATTGGAAATTCCACGAACCGCAAGACGTTTATCCATTAAACGACGCTTGAGAATTAGCAGACAAAGAGTTAAGAAAATACGGAATGGAAGCAGATTTATGGGCGTTTGACATTAATAACATTTATGAAATGCAAAACGATTTTTATGATATTGAATTCGTGGGCATTGACGCCTATGGTAGAGCCTTCATCATAAGCAGAGAAGATGCAGAAAAATATTATGCAAAGTACTATTTAAAACAAAACAAGTTAGTTGAAAAAGGAGATAAATAACTATGGAAATAAAACCATTTAAGATGAATGATATATTGTATATGACTCAATATGACGACGTTTGATATAACGATCATAAGGTACTAAATTATTTTAATAATACTTTATTTAGTTTAAATTCAGAAGAAGAACAAAAACTATATAAAGAACAAATCTGCCAAATTTCTAAACAAATGGAAGAATCAGATGATTTTGTAAATCAATGAATTTATGATAAACTAATAACCTCTGAAACACGTCCAAACTATTTGGAAGGATGTTCTAATATAATTAATACCCTATCTTCAGATAATTGAATAAATGAAAGCGATTGGCTTAAATATCCTGCAAGTCAATTTATGGAAGAAAATGACATTATTCGCAAGGATAATTTAGAAGAAATAATTGATGAAGATTTAAAAGCTAATGGTTATGAAAGTCAATGAATTAATGGAACAACGAAATTTAATGTTCTAAATAATTTGTCAAATGAAGAATATGTTTATGTAAATCCCTATTTAGAAATAGAACCAATTAATATTAAAGAAATTAAAGACGATTACATAGAAGCATTAAAAACTTTTGATTTAGATGACCTTTTAGAATCAAGAGATGAAAAATCATTAATTAATTTAGATCCTAACAAATCTTCTAAATCATCTTTATCTGATTTTGACAAGACCCCAACCGATCTCAAAGATTCCAAAGTAATCAACGAATATAACAATTCATCCCATACCCCCTCTGATACCCAATCAAAATCCCATTCATTAACAAAATAAGAAGGTAATATGAAGAAAAAAATTTTCAATCGTGATCAAATGGAACAAATCCACATTGGTTTAGTCCAAGGTCTTGATGTCACTCAATACACTCAACTAGACCCTCAAGGCCATCCAATATTCAATGATAATCAAATGACCGAAATCCGTAAGGGTCTAAAAAAAGGCCTTGATGTCTCTAAATATAATCAAATAGATCATGATGGATATCCGATATTTAATTGAGGTCAAATGTACGTAATCCGTGAAGGTCTAGAACAAGGCCTTGACATCTCACAATACACCCAACTAGACCTCAAAGGCCATCCAATCTATGATTTTTGACAAATGGAACAAATCCGCATTGGCCTAGAACATGGCCTTGACATCTCAATTTATGCAAAACAAAACAATTTTAATAGACCTATCTATGATTGTTTGCAAATGTCTGAAATCCGTGAAGGTCTAGAACAAGGCCTTGATATATCTCAATACAGCCAACTAGACCATAAAGGTCGCCCAATCTTTAATCGAGGTCAGATGTCTGAAATCCGTGAAGGTCTAGAACAAGGCCTTGATGTCTCACAATACACTCAACTAGACCTTCAAGGCCATCCAATATTCAATGGTTATCAAATGTATGCAATCCGTAAGGGTCTAGAACAAGGGCTTGACATCTCTCAATACACCCATTTGGCCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTCAAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCGGAATCCCAATCAAAATCCCATTCATTAACAAAATAAGAAGGTAAAAAATGATAACCATTATTCTTTTAATTATCATAATCAGACATATAAAAAAGCGCAATAAATTATTAGCAAATTCTAATCAAGCTAATGATCAAAAAAATAGTCCCAAATAAAAATTCTTATTAAATTATGACAATGATTATTCAAAGCTCTTTAATTCCACTCAAAATCCCATTCATTAACAAAATAAGAAGGTAATATGAAAAAAACAATTTTTGATAATGACCAAATGGAACAAATCTGCCTTGGTCTAGAACACGGCCTTGATATCTCTAAATATACTCAATTAAATCATAAAGGCCACCCTATATTTGATAGCTTCCAAATGAATGAAATCCGCGAAGGTCTAGAACACGGCCTTGATGTCACCCAATACACTCAACTAGACCCTCAAGGCCACCCCATCTATGATTGATGACAAATGGAGGAAATCCACAAAGGCCTAAAAAAAGGCCTTGACGTCTCAATTTATGCAAAACAAAACAATTTTAATAGACCTATCTTTGATTGATTACAAATGGAACAAATCCGCATTGGCATAGAACAAGACCTTGATATATCTCAATATAACAAACTAGACCCTCAAGGCCATCCAATTTTCGTTGGTAGCCAAATGTCTGAAATTCGTTATGGCCTAAAACAAGGCCTTGATGTCTCTAAATATACCCAACTAGACTCTCAAGGCCACCCTATATTTGACAGCTTTCAAATGGGTGAAATCCGTAAGGGTCTAAAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGATTTTTGCCAAATGGAACAAATCCGTGAAGGCATAAAACAAGGCCTTGACGTATCTAAACCCACTCAATTAGTCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCCCACTCACAACCTAATCAAAATAAAACCTCAGAATCAAAAAATATAAATAGATGCATATAGATGAATTTATTGATAAATATCGCAGGAATTTAAAACAATCGGGTTTTAATAAAGACAATATAGACTTCTTTTTATATGGTTTAGAACTAGGATTAAATATAAAAATAAAATCCAGTAGAAATTATTACAACTTTAAAAAATCTATACATTATTCTTCAAATAATTCTCATCCCTCTTCCCTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCGGAATCCCTATCAAAATCCCATTCATTAACAAAATAAGAAGGTAATATGAAGAAAAAAATTTTCAATAGTGATCAAATGAATGAAATCCGCATTGGCCTAGAAGAAGGTCTAGACGTATCCAAATACATCCAATTATCCCCTCACGGTCGCCCAATCTATGATAGTTATCAAATGTATGCAATTCGTAAGGGTCTAAAACAAGACCTTGATATCTCTCAATATACCCAATTAAATCATAAAGGTCGCCCAATCTATGATGGTTATGAAATGTCTGTAATCCGTGAAGGTTTAAAAGCTGGCCTTGATGTCTCTCAATATAACCAACTAGACCCTCAAGGCCACCCAATTTTTGATTGAGTTCAAATGTATGCAATCCGTGAAGGTCTAGAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGCTGGTTATGAAATGTCTGTAATCCGTGAAGGTCTAGCCCAAGGCCTTGATATATCTCAATATAACCAACTAGACCCTCAAGGCCACCCAATTTTCGTTGGTAGCCAAATGCACGAAATTCGTAAGGGTCTAGAACAAGGCCTTGATATATCTCAATATACCCAATTAAATCATAAAGGCCATCCAATCTATAATGCTTACCAAATGCACGAAATTCGCATTGGCCTACTATTTGATTTTTTAGGATCATGAGAAGAAAAATCATTAATTAACTTAGATCCTAACGAATCTTCTAAATCATCTTTATCCGATTTTGATAAGACCCCAACGGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCTGATACCCAATCAAAATCAAGAAAGTTATAAGGAACAACGTGTCAAAATACTTTTATAGATTAAGAGGGAGTCAATCTACAGGGAAGAATGCCAAAACATATTCGTTTGGCTCTGCGCAACAATACAATAAAGCATTAATAATGGCTCAAGATGCTATTTCAAAAAATCAATTTAAGGAGGTGCAAATAGAAAGAATACAAGTTATAAAAATTATTAAGCAAAATTCATAGTGTAGGAGAAACAATGATAACAATTATTCTTTTAATTATCATAATTAGACATTTGAAAAAACGCAATAAATTATTAGCAAATTCTAACCAAATTAATAGCGCTAAAGGAGTGATAAATAAATAATTCTCAATTATTTCAAATGTCAATAGATGATGAATGATATACTCAAGAAAAAGATATTAAATACTTTTTAGAAAATTTTAAAATAGACAAGAAAAAAACCATTTGATGCCCCTTTGATACACAGCAAAGTAATTTTGTAATTGTTTTAAAATCATTGGGCTATAAAGTAATATATTCGCATATTGATAATGGGCAAGATTTTTATAAATATGAGCCTATTGAAAATTATGATTTAATAATATCGAATCCTCCGTTTAGAAATAAAGCAAATATTATTAAAAGATTGCAAGAACTAAATAAACCGTTTGCTTTAATTTTTGGAGTTCAATGTTTTAATTCTGGCGGTTTTGTAAGCCAATTGCAAAAACTAAAAAATTTAGAGCTTGTGTTCTTAACAAAAAGAATTAAATTTCTTAAAAATTATAAACAAGACCTTAAAAACATTCCTCAGCCTACTTTTCATTCTCTATGAATTTGTAGTGGAATTACAAATAAACCTCTTTCTATTTTAGAAGGCGCTAAATAAAACAAATTATAAATATTAAATATAAGGAGGATTAAAAATTACTAAACAACAAAAATTACTATTGCCTTTTTGGCTAACTTGAAAAAGTTTTATTTGATATCTTATATTTTTAATTATCGTTTGATTATTTTTAGCATTTTATTTTTCGGGTATGACTATTTCTGTTGAAAAAGGGTCATGGACAAACAAACAATTCAATATTGCACTACTTATAATTTGTTTAATATTAGCCTCAACATTAATTTATCCATTTATATGAGGTAGAAAAAGACGAAAATTTGCAAAATCACTTGTTTAGTTTTATAATTATAATATACTTAAAATAAATAAAAAATAAGCAAATTTCAATACAAAAAATAAACAAATAATAAAGAATAAAAATTGTCATTATAAGACTTAAAAAATATATAAATAATATAAGGAAGTATATGAAGAAATCAAAAAGAATTTTATTAACAATAGGCTCTACAATTGCCTTAACTACTTTACCAATTATGGCAATGAGTTGCGAATGACTTGATAAATTGATTAATAAAAATAATCCTAATCCTACCCCAACACCGGGTCCAGATTCGGGTTTAGTAAATCCAAATCCTTCAAACCCAACTCCTGCTCCTGCTCCAGTTCCAACCCCTGAAGTTAAACCAGAAGAAAAAGAAGGTTATGTTATTTATAAATCGACAAATGAAGATTATTTAAAAAAGAATAACACTTATTATTTTAGCAAATTGAGTTATAACCCCGTGGAATTGTCTGATGAGATTTATAATAGTCAAAAATGAAAACTATACAGACCTAAAATGTGACTTTCTTTAAAAATGCCTAAAGAAAATAAACCAGAACTTGGACCAGAAGAGCAATTAGCCAAATGAGGTATGGATTTAAATTTAAATGCTGACCATTTTACAAATGACTGTGAAAGCGCTTGTGGAATTTGAAACATTATTAGAAAATTGGCTGAAAGTGATGAAAACAAAGAAGGTATCAACCAAGGCGGTCGTTTTAAACCAAAAGATAATGAAAGTTATAATGAAGCCTTAATGAGATTTTTAAAAGAAATGGATGATTATTTAGATCCTGATATTGCATTTAAAGGTTTAACAATGGATGAAACTCATATAAATTATGTAACGGAAAATTTAGCTAAAGATCCTAAATGACTTGAAAAAAAACTTATTTACTCTGCTGATTCAAAAATGCCTTCTACTTTTATTAGAGACGAATTTAACCACCAATCAATTGAAGACATGGATGGATTTGTTAAAGAATTAATATCTGATTATGAAAATGCTATGTATGAAGGATGAACCGTTGAAAATAAAGCAAATGATTGGTTTGTTGCTCCTTTTTCATTTGGATCTAATCCTACAATGAGAAATAAATTTCCACAACAAACTTATGATATGTATACTGATAAAAATGGTCTAGATAAAACAACTCCTACTCCTGCTTATAATAACTACAATCCAGTAGCATTTAATTTCTTAGTAATGGAAAGATTTGACTTATCAGAAAAATTAAATTTTAAAGTTTATAAGAATTTAATTCAATGACTAAAATCATTTAATAATATAAACTCTGAAAATAAATTAAAATGAAGTGAAATCGAAAGTGATAAAAAAAGAAAATTAATGAAGGCTTCAACTGAATTAGTAAAAGCGTTAGTGGAATTTATTGACTGACATCATTTACTACTAGGAGAATTTGAAGATCCTAATGATAGACATTCAACTTTAGCTGATTTGTTTGCAATGAAGAGTTTAACAATAGGTGACAAAATTGACTTTGATTACACATATAGATTTGCTTATAAATTTTTATATGAAAAATTAGTGCCTACAATGGTGGCAAACGGTTGAAACATGCCAATTGAATTTAATAATTTAGAAACGCTTATGCTAGACAGAAATAAAAAAGATTCATATATTTATGACTATATCTTTAAATATATAAATGTCATTTTTAAAGCTAAATATAAAGACATTGATGAAATCAAAAAACCAAATGGATATTTAGATTTAAATAATAAAGAACATTGACAAAAATTATTTTTAGACAATGTTTTAAAAAAATGAAATTCAGCGTTTAATATAAATGTTGATTTAAAAGAATTTTAACTTATATAAGGTGTTTAAAACACCACAACTTATTTTTAATTTTGAATGATAGAAAAAAGCAATTTGATTTTCAAATTGTTAGTTTTCTATTTATTTTTTTAATTTTTAAAATAACTCTTTAATGAGTATGAAAGGAAAAAAATGAAAATCGATGATAAAAGCTTTAGCTTTTTAGGAAATTTTAGTGAAACTATAATGAGGTCTATTTTTATAGCCCTTATTCCAGTTGCAATTATTGTATTAATAGGAATGATATTGTGAGCAAGAAGTCAAAATGCTGCCACAAAAGACCCTAATGAAAGAAAGCAAAATAATTTAAAGGTATGAGGTGCTTTATCTGCACTAATTATTTTAGTAGTAATGTGACCATTAATTGAATTAATTAAGGCTAATGTTAAAAAACCATCTAATGTTGGCTCACAAGCATCATTATTTACACATGCAATATTAAATAATTTTACAATTTCAATACCTAAATTGACTAATTTTAATTTAATGGTGTAATATGAAATTTTTACAGGCGATAATAAATCCTTTAATAAATGTAATCGCAGCGCCTATTAGGCTTATGTGGTTTGTACCTCTTGCTGCTATTGGATTAATAGGATTTACTATATTTAATCTTTTGTTTTTCTTGTTTAAATTGTTTAGCCTCGATTTAAACAACTATTTAATGTTTAATTTATTACCAGGCCAAAGTTATAGCCAATTTTCTGTAGCAAGGCCTTGATATCAATTTTTAATTATTAGTTTTGTATTATGATTAATAAACTTTTTTGTATTTGTTATTAGGTATTCAAAAGAATCTAATGGATTAAAGGCTAAGGAAATATTTGTTCAAGCCTTAAAATCAACGGTAGGAGGCGTCCTCACGCTACTATTATTCCAAGTAGTAATTCTCTTTATAAATATTATCTTTATTGAATTAACAAAAGCCTTATATGGAGATTATAACGATTTCTTTAATTTTGGTTTCGGTCAAATTTCAAATAATATTATTAGAAATATGATGCCCCCAAAAGCCTTTGTACCTTCAAGATATTGAGATCCATTCCCTTACGGATCTCCTATAGCAAATCCTTTGTGAGGTTTGGGTTTTCCTGTCTGATGAAAATTTACAGAAAACATTACAGGTTATGCACTTACTATTGGCTTATCAATAGCTATTGTGGGTGTATTAATATCAATTCCTTTAATATTTGTATTGTTTGACATTATTTCAAGAGTATTTGTTAATTGATTACTATTTATTTCATTCCCACTAATTCCTGCATTTTCTATAAATGATGGAGGAAAGAGAATAAGAGTATGAAAGGAAAAATATTTTGGTAATTTATTATCCTGCTCGATTTATGGCGTGGGAATTATGATGCTGGGTAGTTTTATAGGCTTAGCTAATACATTTATGACTAGCGCTACAAATTATATGATAGGAGCCTTTTCTTCATTCTCAAACTTTACTACTATCAAAGCCGTTTTAGCATTATTATCAACTTGTTTCTTGGTAATTGGCTCTGCATTTGCTTTCAAAAAATTATCAGAAATTGCAATTGCTTTTGTTGGAAGTGAAGTTTCTGGTGGCCAATCTGCAAGACTTGCTATGTCTGGAGCAAGATCAGGGGCAGGAGCATACAAAACAGTAAAGGATGCAGCATTAAAGGGCACGAATGTAGGTGCAGCATATAAAGTTGCAAGTTTTCTAAAATAGCAAAGTATAAGGAGTAATATGACGCAATTACAGCCAAGTAGAATAAAACATAGAAGTAGTAAAATTTGAAAAACTTTCAAAACAAAAGATCTTATTGAATTTGTTGTAATTCTATTATTATCATTTTTTATTAGTTTTGGTTTTAGCCAATTAATAAATGTGAAATTAATTTTCTTTGCATTTTGAATACCTACTTTTTTATTAGGAATTATTTTAATAATGCCAAATCGAAAGAATAATTGCAAGGTATATGTTTTAATATGAAGAATGTTTGTTTTTTGAACTATACCAAAACGGTTTGTAAAAAACGTTCAAAATAATTCATTTAGAAGTAGTTTGGATTTAAATCCTTATTCATGTTTAGTTAATGAATATGTAGTCAAAAATAAAAATTCAAAAATAAGTTTTTATAATCCAAGAAATGATAAAGAAAACTATTTAGCGGTATTTAAACTAAATGGTCTTAATATTTGAAACGAGGATGAACTAACTCGTGAAGCATTTATTGATAGTTTTGCAAAAACTTTAAATATACTTACTTACAAAGTTTCTTTTGTTAAACTTGAAGAAAAAGTAAATTATGAAGAAAATATTAAATATATTGAAGCCAAACAAGCAAAGCAAATAAATAATAATGAAATGTGAAACAATTATTATGACCTTAATATAAAAGATTTCTCTAATTTAAATAAAGAAGCAATAAGTAATAACTTTTATTTAATAGTAAATGGTGAAAATCTTAATGACTTAAATGAGTATCTCGAAGTTATAATTGATAAATTTCAAGATACTGGAATAGGTTTAGAAAAATTGCATAAAGCAAGACTTCTAACATTTCTTAATAAACTTAATATGTTTGGTATTGATAGCCAAAACATACAAGAATATTGCAATGATTTAGATGCTAAATTTGGAGTTGAAAATTCATTAGATAACTTATTTATTTATAATCAAATTATTATTAAAGATAAATATTTACAAATCAATGATAATTACGTAAAAATCAATTGTTTAAACAAATTGCCATTTGAATTGTCTGATAAATGAATCAAAACCTTATTTTCTATTAACGGAACTTGTATATGAAATAATTTCCCTTGAAATAACAATTTTGCCAATAAAAAAATGGACAAATCCATGATGGCATCTATTGATACTACTAACGCAACAGGATCGGTGTCAGCAAACTTGGTTGGTTCTATTGATGAACAAGCGACAATGGCGATGATGTATCAAGTAAATAAAGATGAATACAAACTATTTGATAGTAATTTCTTCTTTGTATCAAAAGCCACTGACCTTGCTGAATTAAATAGACAGCATAGAATTGCTAGAAATATAGCAAGTAAAAAGAATATAAGCTTAAATGACTTAAAATATAGACAGCTTTTGGGTTTATCTGAGGCTTGCAATTATCCTTTTGAAAGATTAGAAAGAGAATATTATCAAATTACATCTATTAATTTAGCTTTGGGATGGCCTTTTGAAACCGAAAACCACAATGATTCAAATAATTTACTTCTAGGTTACTCAGAAGCCACAAGAAGTCCTCTAAGTTTTAGATTATTTGATATGTATCATTCAAATAGAACTAATTTTAATATGTTTATAAATGGTACTAGTGGAAAAGGTAAAACAACTTTTACTATGAAGTTATTAACCAGCATTTTAGCAGCAAATAATAAAGTTGTTGTTATTGACCCTCAAGGAGAATATAGAGAATTAGCTCAAAAACTTAATGGACAAATTATTGATTTGGGAGCAGGTAAAAACACCATAATCAATCCTCTTCAAATCCGTAATTCATTAAAGGATGCAACGGGTAATGATACATCTTCACTAATTAACAACCATTTGTCGTGATTAGAAAGTTTTTTTAAGGTTTTAATTAATATAGAAGATCATAAGTGAATATTGCTTCAAAACGTAATAAAAGAATTTTATGAAGATAGAGGAATATATAAACTTGCTTCTATTGAGCAATTAAAGGCCGTAGAATGGCCAACAATAAGTGAGTTGATAAAATATATGTCCAACTATAAAACGCTTCATTTAAACAATCCTGAAGCAAAAAGAGCAATATTAGTTGAACTTTGTGAATCGTTTAGCTTTTTGTTTGAAAATAATGGAAGATATCAAAACTTATTTAATGGACATACTAATATAAAACTTGATAATGATTTTGTAGTATTTAATACGCAAAACTTAATAAATGGAGCGGATAATATCGGCGCTAAATTAGGAACAATGTGCTTATTAAACTTTGTTAGTGAAATAGTATTTAATAATTGAACACATAATGAAATGTTAAAGAGAAAATATTTGCAAGAACATAACATTAAGTTAATGGATGCAAAACAAGTAGAAGATACTATTAGATTTTGTGCCGTCATCGTAGATGAAGCCCATTTATACATCGACCCTGATAATCCTGCCATTTTAAAATATATGGTTCAAATGACAAAAACGGTTAGAAAATTCAATGCAGGTATGATATTTACTACCCAAAATCCTGGTGATTTTACTATTAATAATGCCACAAGTGATGATGCATCTAGAATTATTCAAAACTGTCAATATTCTGTATTCTTTGGCCTAAAAGACCAAGATATTGACAAAGTGCAACAATTATTTAAAAACTCTAATCAATTATTACAAAGTGAAATTAGATATTTAGTAAATGCAAGATATGGAAAATGTTTATTTTCAATAAGTTCAAATAAAAGAATTAGAGTTGAAATTTATTACAACAAATTAGAACAAGAATTATTCTTCAAGTTCTTAAATAAAGAATGAATTTAATATAGCACATGTGCGAGCATAATACACGCACACGTGCATAAGGAGGTTTAGTTGAAAACTAATAAAAACGAATTAGATCAAGATTTTGATAATTTAGATAATAATGAAATTTTATCAAATGACAATCAAACTCAAAATGAAGATGACTTTATAGAAAATATTAATTTAACAAGTGATAATCAAATAAATAACATTATGAATTCTATCACTAGTTCCCAACCTAAAAAGCAAAAGGAAAAAAAGCAACAAAACAATTTTGAAGCACAATTTTCAGGACGATTTACTGATCCTGAAGATGTAGGAATGTTTTATCGTTGAAAAAAAGAATTAGAAAAGAATGGAAAAACTGTTTATAAAGAATTAAACAATGTTATTAGAAGTTATTTATTAGCAAATGAGAAAAAAGAAATTATGCGGGATTGAAAACATGATTTGTTTCAAGCACTAAGAAAAGTTAGTTGAAGTGCATTAAGTCCTTATCAATTGCAAATAAATCGAAGATTTAGAAGCATGGATATAGAAATGTCTATTATTAATTTAAAACTTAATATGATTATTAATGCTTTATTTAAAGATAATGGAGATTTAGAAAAACAAATTGATAATCCCGGGGCTGATATATTAGCAGAATCTAAGTTCTTTGAACGCACTATAAGAGGTGCTGTTAATAAATTAACTAATGATTGAACTAAAAAAATGGCAAATAAAATTCAAAATTCAGAACGTGCTTGAAATGATTTTCTTTCAAGTTTGAGTGAAAATGAATTTGATGACATTTTACAAGAAATAGGGGCAACTAATTCTACTGCCCAAGAACAAATAGAAGAAAAATAAAAATATATAGCACATGTGCGTGCATAATACACGCACGCGTGCAATGGAGGATAAATGAATAAAGTTATATTAACTGGTAGGCTAGTAAATGATAATTGAAAACCTTTTAAAAGCGAAAAAGGACAAGCATTGCCCTTTGTTATTGCACTATTTGAACAAGCAAATAAAACCAATTTTATCGAGATTACTTGTTTTAATAAATTGGCAGAACATGTTATGAAATATGTTAAAAAAGGAGATTTATTAGAAGTTGTTGGCAAATTACACAATGCTAAATTTAGTTCAAAGAATAACGAAACTATTTATAAAACAGAAATAATTGCTAAAAATATTACTTTTTTGACAAAGTCAAAATCAAATATTCAACCCATTTCAAAAGAAACCAACTCAGAAAATAATGAAAAAGACATTTTTGGAATAGATTTTTTTGAAGATGAAGAAAATTAAAAAAACCTTATTGATAGCCGGAGGAATATTTAGTATTGCTTCGGCTTCTCTATTTTCTTTGAGTTTTTCTAGAAATGAACATTATTCAAGTTATAAATATGAAGAAATTGACAAAGACCAATATTCTTTTTTAAACAACAAAAATTTATTAAAAAGTTTTTTTACAAATTTGACATATTGAAAAAATAGCAGGCAAGATAGTTTTATTTTTGACCCCAAAAGTCCTACAAGTAATCCCCTTTATTCTGATCAAAGATTTATTGCAAATACCAATGATAGATTAGAATTTTTAAAAGAATTGTCTATTAATGATATTAATAAAATAAATAATCAACCTTTTGGTGATTATAAAATTACTTTACAAAATCAAGAAGAGGCAAAGGAACAATTGCCAAATGTAAAATGGTGAGCTCCTAGTTGATTGTATGGCTACAAAAAATATATCAACGACTTAACTTTTTATCACGGCGATGGTAATGAGTGGTGTGATCAAGATGGGTGCCAGGAAGAAACACATAGCAACGGCGAAATTACTCAAACTATTAAAAAATCTAATTATAGTATTAACTGAGCTAATGCCCAATCTTTTAAAAAATTCACCAAAGATTATGATATAAATATTAAAAAATTCAATAATAGACTTAGTGATCCAAAAATTAGTTCGTCTGATATTGCATATTCTGATGAAGGTTATACTGCTTATAATAAATTTAAATTGTCATACAATGATTATTTAGAAGTTAATTATGATTTTACAAAGTGACATGGCATAGATCTTGAAGATGTTTATTCTTTAATTTGCTTTTTAGCAAATTCTAATAATTTTAAGCATTCTCAAAAATTCATGTCAGCATTTCATCTACAAAATTCAAGTCAAGAAATTATTATAGATTCTGATACTATGGGAAAATTAACGACAGGTATTGATTTAAATATTTTTAAAGGTGGTAAAAGTAATCTTGAAAAACTACAAGAAATTGTTGAACAAATGGATAATGAAACAGCAATTGGCAATTTTATTATTAAATATGGTTATGAAGTCAAACCTATTTCAGATGGAAATCATTTTAATCTAATTTTTAAAATCAAACAATTAAAATATAAGGGAAAAGATATCACTAATTCTTTTGCTTCTAAAATTTTTAATGAAGCAGATACTTATAACTTTGCTAATAATTTTGCCAAAAATCAACAAAGTAAAATTCAAATTAAGAATCTTTATCATATGTTTTATAGTCTTTTTAAAGGCAAGACAATAATGGCTAATGTTCCAGTAAGATTTACTCCAAGCCAAATTTATCAAACTTATGCAGACAAGACAGGATTTGTTAGTAGATTATGATTTAAATATGGTGTAGTTCTTAATCAAAATAACCTATCTAGTATTCAAGAAAATCATAGTTTCAATTTCAAAGACAACCTACAAGAAGACAAACCTATACAATTAGAGCCTGCAAATGGTAATTACGGGGGAAAATGGTTAGTGCATACTCCACTACAAGTAAATTTTGACACCACATTAGAAGAAAATGAAGTTTTATTTATAAATGGTCAAAAAGTTGATGTTTTAAATAGACATTTTGTTTATCATTTAAAAGATTTAAGACGTTCGGCATCCGATGAAGAAAAAATAGCATTAGAGAAAAATGATTCAAATAAGCCTCAAGAAGAGTTAAATGACGATAATTCAAGAAAGAAAAACGAGTATATAGTTGAAATAAAGAAATTCAAACCTGGCACAAATAATAGCGGAGAGCCAATAGCAACTTATAAAATTAAGTTCGTTATAGTTGAACAACCAATCAACCAATCATTTAAATGGTTTGCTTGAGACCCTGAAAACAACCCAAAACAAAAGGAACTAATTACTAAAGAAATAACAAAAGATGGAAGAATTTTAGTGGATAAAGAAGGTAATCCATTACCTAACCCTAAATATGATCCTACAATAGACCCTAAAACCGGTACTAAAAATCAACTCATATGAATAAAAAACGGAACTTTAGCTAAAGAAATAACAAAAAATTTAAAATTTTGATATCCCGATATTAAAATGTTGAATGATTTTGGTATTTTTGCAGAGGGCGCTGTCCTTGGTAAAGGAGCCTTAAGAAATCTAATCTTAGATAAAAATATTGCCAAAAAATCTAATGTTACATACTATAAATGCAAAATTTATGATAAAGGAAGCAACAAATGGCTTAGTCCTAGTGAAATTAAACCTGAACCATTATCTTCATCAAACGGTAATAGTGAAAATGCTTATATGTCTGAAGAAGGCATCTGAATAGTTTATGCTGCTACTTCTACATCAATTTCAAATATTAAATTAGTGCTTATCGATGAATCAAATTCACCAAAAAATCTTTTCTGGCAAGAATTAGAAAATAAAAAAGATAATTATGGCATTGATTTAATGAAAAAATTTGAGCCATTTTGACAATCAACACTTGGAATATATTTTAAAAAATGGTTAATTGAATCTAAACATTATGATAAAGAACAATTAGACAAATTAACATATGATGAATTATTGCCTCTTTATAACGAATGCGTTAATTCATCTTGAAAATTTGCTGATAACGATAATAGCAGCATAGAAAATGCCATTAATATTTTTAACGACTTTAAATGGAGTAAATTACAAAATTTTAATGGTCTAACTGATAAACAAGCCATAAAAGACAAAGTTCTTGAATATATTAGAACAAATATGTCAAGCAACACATTAACCAAAAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACCGAGGAAAATAAATTTCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGACACGTATATAATCCACCTATTGATTTAAGCACGTTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCAGAAGATGTTATTGATGAAAGATATAGAAAAGCAATAGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCCAATTTAAATGAAGTAATTGCCCATTTAATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTCTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGATTGGAATTAGTTTCGAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAGGCTCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTCGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATATAAGAAAATTCTTTTATTACCTACAAACAAAACCACCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATAGACCCTGATTGAAAACCAAATCCAAATCCCGATGATGAGGATAAGGCAGTTAATATTTTTAACGACTTTAAATGGAGTAAATTACAAAATTTTAATGGTCTAACTGATAAACAAGCCATAAAAGACAAAGTTCTTGAATATATTAGAACAAATATGTCAAGCAACACATTAACCAAAAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACTGAGGAAAATAAATTCCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGACACGTATATAATCCACCTATTGATTTAAGCACGTTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCAGAAGATGTTATTGATGAAAGATATAGAAAAGCAATAGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCCAATTTAAATGAAGTAATTGCCCATTTAATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTTTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGATTGGAATTAGTTTCAAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAAGCCCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTTGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATACAAAAAAATTCTTTTATTACCAACAAACAAAACAATCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATTGACCCTGATTGAAAACCAAATCCAAATCCCGATGATGAGGATAAGGTCAGTCCCGAAGATATACCTAAAGACCCAAATAGAATAAAGAAAGTCGTGAAAACTTGATGATTTCCTTTAGTGGTAATTGTAGGTATATTAACATTTTTAGTTGCTTCTATTTTCATAATTAAATCTTTATTAAGGAAATACGGTTGATCATTTGGAAAAAGAGCTAAACGTATTAAACGTCAAAATGAAAACAAGCAATCTTATCAAGAAAAACGCTCAAATAAGCGTCAAGAAAAATTAGCCAAAAAGGAAGCAAAAAATTTAAAACGCCAAACAAAAAAACAAGAAAAAGCAAGCTTAAAGAAAGAAAAAAGGACAAAAAAACAAAAAATAAACCAAAAATAAATAATGAGCAAAATTCTGTAAATTAGCAAATAACATCAAGTGTTCTTGCACTTGGTGTTTTTAATTAATTAAATCCTTTAAAAATATAAAAAAATCTCTTACTAAATTAACTTATAATTTTTTTAGTAAAAGATTATCAATTTGTTTAAGAACAAACATATGCAAAAAAGATTTGAAAAATTTTTAATTTTTTTGTTTATTTGTAGTATAATATAACCAATAGTCTAAGAAGGATTTTGTTATATGAGTAATCGAACAAGCAAGCAGCTCATATTTTATCTTAAGTGCTTGATGATACGCCTTAGACTCTAGTTCGTCTTAAAAAGAGGTTTATTCCTCTTTTTTTGTATAATTTTTATATATGTTAGAATCATGAAAACCATTAAAGATAAAAAATCCTTTACTAGAAAATATTTTTAAGTTGCCGATCGATGGCAAAGTTTTAAATAACGGAAAAATCTAAGCTCACCCCCTTATATTTTTTGAAAATATTATAAAATTAAAAATTTATTTGTAGTATAATTATTATAACTTAAGAAAGGATATTTATAGGTAATCGAATAGTAGGCAGCCTATAAACTTTAATTTTTTGTAAAGCCTAATGATAGGTCTTAAGTCTTAGTTCGTCTTAAAAAGAGGTTTATTCCTCTTTTTTTGTATAATTTTTATATGAGTTTAGAACTATGAAAACCATTAAAAATTACTAATCCTTTATTAGAAGATATTTTTCATCTAAATATCGACACCAAATTTAAAATTCATCCTTTGATAATTTTTGAAAATAAAGAAAATAAAACCTATTATTGTATAAGACTTCAAACATCTACCAAAACTACAAAAAAGCATAATTTACAAATTGATAATAGTTCTTATCAAACTAATGAATATTGAATGAATCATCAAAGTGTAGTAGTCACCAAAGATATTTTTATAATTGATAAAGATGTTTTAGAAGCCAATGTAGATCAAAACATCTATCAAGAAACCTTGACATTAAATAAAAACGATAAATCTTTAATAATTAGCGATTTAGAACAAAGGATTAATTCAATTCCACCTGATTTAAATATTTTAAAAATATCTAATAACTTAAAAAGCAATTTAGTTTTATACACTAATGATAGCCTAGTTAATTCTCAGTCTAATTCGATTTTGAAAGGCAAAAATTACAAAATTAAAAAGGCTACTAAAAATTATTACAAACATAATTTTAACAAATCTCAATTTCTTAAAGATACAAACACGTCAAATATAGAACATACTAAGCAAGCCCTAAAAAACATAAAGTTATGCATAAATAAAGCTAAAAAAATTGATTATACTAATTTTGAATATATTTTAGATGTTCAAACAAACACATATCTATTTCATCTAATTCCCTCTTCTCTATCCGATTTTGATAAAACACCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACCAATCTCAGAAAGAGTCCAAAGCTAGCCAATTCCGCTCAAAACCTCATAATCTAAAACATTAGGCAAGAATATTCTTGCTTTTTTGTTTTAATTTTTATATGGAAATAGATTTTTATATTAATGGTGTAAGCGAAAAAAGTATCTTATCCTCAGGCCAATTAATAGTAGAAAAATTGAACATCGAAGAAGAAATATTTCGTACTAAAACAAGGTTTGAAAATAATCAATATAGTAATTGAATTGTATTTGAAAGAAAGCATAAAACATTAATAATAAATGGAGAAAAATTAACAATTAATATTACTAGATATTTAGACAAAAAAACTAACAAAAAATTCATTTTTTATCATAACGAAATTCTTAAAATAATGGGTAAAAAAAGATATTGATTAAGTGATGTTTTAACGGCAATAAAAACCCAAAATTTAAGGCGTAGAAATCCAAATCAAAACGAAGCATTAAAACCCAAAATTCCTTATGATATTTACTACTATTATTTAAAGAAAAAATACATTACTTTTTTAGTAAAAAATACTAAATTTTTACCTTCAAAAAATGATGTAATTCAGCTAGAAAATGATGATGCTTATATTAATTTAAATAAGGGAAAATTTATGGTTAGAATGGTTGGATTTCACTCTGGATATTTAAACCAAAATAACCATATGTTATTAAATAAAACCATTATTTTTCAGTTTAACAAAAGTCAAAAAATTTATCAAAATATAGATATCTTAGCCACTAAAACCAGCGAAATTTTAAATAACTTTTACCATGCAAAAACTATTAATTTATCAGGAGATGGAGCAAGAATAATAACTAAATTTAGCGAACAAATTAATGCTATTAGGTATTATGATAAGTTCCATTTTCACAAAATATTATTTGATGTTTTTGGGTATTCAAGAAAGAAAAATAAGGATAATAAGAGAATATTTGATAATACATTTGGGAATCAATTTTCTCAATTTTTGTGATTTTTAAATAACAAAAAATATGAAGATTTTGAAAATAAATTACATGAGAATTTGATTTATTTAAGAGAAAATAAATTATCTGCTATAAAAATTGTTGAAATAAAAAGTCTAATAAAATTATGAAAAAACAACAAAAATTACTTAATTAATAGCTATGAAAATGCTAATTATTATGGAGGAAATGCCGAAACTTTTATTGGACATTACCTCAAGAAATATACAAAAACAGCATTTTCGAGACATAATATTGACTATTTAAAACTAAAAATCTTGTTAAATATTCCCCAAAATTTAAATGTGGTATTTTTATAAAAACCACTTTTTTTTATATATTTTAAAATCCCTTGTTTGCTAATGCTACGCTATTATTATATAATTATTTATAATTATATAAAAAATTGAATTGATAGCAATTAGCATGCTATTTTTTAAAAAAACTTAATAATGCAAACTTTTGTGCATACTATTATAATATATTATTACAAGTAATAATATATTAAAAAAGGAAAATCCACACTACGGTAAGTAGTTTCCGTGGTTCTTCCTTTGCCCTATTCAAGGGTGCGCCCGTACGTACGCGGGCGCGTAGAGCATTTTTTAAGTTTGCAATAAAATTTGCATCAAAAAAATGCGAATTTTTTAAAAAATATCTTTAATTCAGTATAATTAGGGTGATAGAAAAATCTCTTTATGATTTTGCGACCCCTGATTTTCAAACACTTCCAATCTTTTTTGATAAAAATAACGACATTATTAAATTATTACCAGCAGATTCACAAACAAAAATTGATGATTATCTAGACAAAATTAAAAAAATTACAGAAAAGAATAGTCAAGCCAAATGAAGCGCGCCAACATTTGACGAATACATTTCAGCCTTTAAAGCACAAGAAGAAATTTTTAAAATTATATCTAGTGATCTAAAATCAGCAGATGTTAACATATTAGAAAAATTGACAAATAATTTAAAAGAAATTAAAAAACAAAAGAAAAATGATTATGACACGATTGTTACCTCTTATTTAAAAGAAAAAACAGAAGAAACAATAACAAAAAATGAATCAATAATTGCTAAAAAATCTAATATTGAAAGTCAAAATATTATTGAAACGTTTGTTTTATTGCAAAAATTAGAACTTGAATTTGCTTACATTAATTGAACTGAATTAAATTTGATTACTAAAATTAAAAATAAATTAGAACTGTTAATAAAAATCAATAAAATAGATTCAAATGAAGAAGCAAAAATATTAAAACCTTGAACAGATTCGCTTAATAGTTCAAAAAAACCAGAAATTTTTGAATTAGAAAACATCTTGAAAGAATTAAGCAAATCTTTAAAGGAACTAATTAACAAATAAAAGTTATTCACTTAATAAAGACATTGTAGTGCAAAGTTTAACCCTCTTTTTCAACGGAAAGAAATTTACATTACACAACCTATAAAAACAGTGTATAAAAAACAAGATAAAAGTTATTGAACAATTATCTTGTTTTTTATATTATTAATTTTATTGCTATGAATATGCAATTTATTGGGCTTTAATTGTTATTTCATCGATGGTTATGGGACTTGCTCATCCAAAATATCCATAACAAGGGATTTCTGGGTCATAAAATTGAATAAATCCTTCAAATCTTAGTTTCCCATCTTGTTCATAAATTCCTTTAGTTTTTGTTCCCCCTAAAAATGTATTAAAATTTACACTTACTTTGCTAAATAATTTAAATAAAGGAAGGCTTTTATCAAAATATAATTTTCTTTCATCACGATCAGTTTTATCAGGTAAGAATGTTTTTATTTTATAAACACTAGCCTCGCTCGTTTTTTCAAACATATCATTAATCATTTTATTAAAAATTATTTTTTTAACTTTTACAAGTGAACCATCAAAAACCTTTTTATGTTCAGCAATAAATTCAGCATTAAATTCGTCGTTTACAAAATAACTTCAATTTAAATATTCGATTTTGCTTCTACTAGTTTTGGTAATTTCTATTACATCTTCGTCCATTATTTTTTTCTTTATTTCATCACTTAATTTTGCTTCTTCTGATTCAATATATTTGAATACTTCTTTTAAATAATTAGCATACATATCAACTATCTTATAAATATTTTTAAGTTGATTTATATATTTCTTACAATCTTCTTTATTTCTTTCATTATAGGCTTTTCTATAATATAATCTTTCTTCAGTTCCCAGGTATTCACCATAAACTTCGTTATTAACTTTTTTATTAATTGCGCTCTTTATAGATTCATTTGATTTAATTAATTGTTCGCTGTATGTCTTATTAAATTTTAGAGAGCAAATTTCATCTAGTTTAGATTCAATTTGCTTCTTTTGGTCTTGAAATTCTTCTTCAATTTTTAAAAATTCATCGCTTGGTGGCACAACTTCATGGCTTTTAGCTTCATTAATTTTTTTAGTTAATGAATTGATTGCTTCTTCAATATTCTTTGTTTTTGATTTAATTTCTGAAATATTTGATGATTCATCAACATTTGTAGTATTTAATACTTTTTGTTCTTCAGAATTATCAACATTTTTTGCCCTTGCAGATTCAATTAATGCTTTTAATTCTTGTTTCTTAGTTTCAAAATTTTGTTTTACTTGAAGTTTGGTTGTATTTATTTTATTTTCTAATTGTTCTTTTGCATTTTTAAGATTGTTTGAAGCATCTTGTATTTTTGAAATAGTTCAATCTTGGGCAATGTTTGCTTCATTCAAGGTTTTGTTTATATCATCAATGTTTCCTACTTCTTTTGCATCAGGGGTTGCAATTAATTCTTGTAGTTCTCTTTTTATTTTTTCAAAATCTTGTTTTGCTTGTTCAAATTCTTGTTCATTAATTTTTTTATTCAATGATTTAATTGCTTTTGAAATTTCATTAGTCTTATTTTTAATGTCTTTAACTAAATCATTATCTTTAACTTCAATATTCTTATATTTTTCTTCTTCGTCTTGTTTATTAATCTTATTGTCTATTGAATCTAGAAGTACTCTCAATTCTTGTTTTTTAATATCAAACTCATTTTTTGCTTTATTTTTGGTATCTTCTATTAAATTTTGAAGATCTTGTTTAGCTTTAATAATTTTGTTTGTTTTTTCTTTAACCTCACTAATTGAAGAATTTTCTTCAATGTTTGTTGAATCTAAAATTTCTTTTGCTTTTGAATTTTCAATTCCTTGGTCAGCGCTAAAATCAATAAGATTTTGTAATTCTTGCTTAGACTTTTTCAACTGTTCAATAGCAATGTTAAGTTCCTTATTTTCGTCTTTCGAAGGCGAATTTTGTTTCTTTTGTTCGTTAATTTTTTGGGTTAAAACATTGATTTTTTCTTCAATGTTTTTAATAATTTCATTATCTCCTTGACTATTATCCCCTTTAAATTCTTTTTCTTGGTTAACTAGTCATTCATTAAGTTCTTGTTGTTCTTCATCACTTAGTTTTGAAATTAAATTTTGAAGTTGTGATTTTAGAGCATTAAACTTTTCTTTAACTTTTACAGTTACGGTACATTTAGATGCTAAAACAGGCACAGAGCCCGCTGCTACACACAACGCTAAAATTTGTAATGATAAATTTTTTTTCTTCATTTTAGTTATACCTTTCGTATTTATTTAAGCTTAGATATATAAAATTATATATATTCATATATATAAAAATATAAAAATATTTTTAAACATTGCAAAAAATATTTTTATTCATATTAAAGTTAAAATCAATATTTTTCGCTAATTTTTATTCTTATATTGAGTTTTAATATGATTATGAGATAATTTTAAAGTAGTTAAGAGGAAAGGGAGCATATGGAAGATCAAAAAGATTTTAATATTATAGATAGTGAAATAGCAGCATTTGGCAATGTAAAAAAGATCTTTGATGAAAATATAAACAATATAAATGATATTTGTAAAGAAATAATTAAACAGGAATTTTTAGAAAAAAATTCAAATAACAAAAGAATTGAAGAAGCTAAAAAACAATACGAAATAGTAAGAGATTCTAATGAAAGAATTATTGCTGAAAAAAAGCTAAAAAAAATATCAGGAAGGAAATTTGCAAATTGCATAATTTTTATACTTTTCTTTATTCTAATTGGTTTTTGTTTTTTTTCATTTTTTGCTAACAATAAGAAAATAATTAAATCATTTGAAGAGTTTAGAAATAATGAACAATTAAAAATTGATGAAGCTTTAGGCAAGGCAAGACAGGCATACGTTGAAACCTTTTCATTACAAACCCTTGAAGATATAAAAAATAAAATTTTAAGCAAAATGGGGATAAAAAAAATATCACAATACGATGCTAAAGTTGGTGATTTTTCGCGTAGTTTTATTTATGATAAAAATTTTTGAAATTTTAATAGCATAAGTCAATATAAATTAAGAAATTCTTATATTTATGACATTTTTTATACTTGCAAATGAATGAAAAATGTTGTTACTAAGGCTTCAATTACCGTTACTAAAAAAGATGCTAATGGTAATACTTATTGAGAAACATATTGAGCATATCATACCGAACCTACACCTGTTGTAGAATATTTTAGAAGTTTAACCATACCTTCAAATTACAAGCAAAAACTATACTTTGAAATTATAGAAAATAATCGAAGTGAAAAAGAAGTAAAAAGACTTTTAAAAAATGAAGGCTTCCACCTTGAAAATAAAGAAGTCTATCAAAATTTTGATTTTGCATTTTCTGATGACCAAAAAATTGATTTTTTAACTTATTTTGATGTTAGAACTCAACAAAACCTAATCAATTATGCCCAATATATGAATCAAGAAAACATGCCGGTATTTTCAATGATAAAAAATGGAAATTGGTTGGTTTCTAAAAATACGATTCAATCAAGTTCCTTATTATTTGAACAAGTTCAAGATTGGATGTTAAGTGTTTTGGATTTAACTGAAGAACTAACAGTTGACTTTGTCACGGAATTTTTAAGTTCAAAAATTATTGAATGTTTATTGCCAATATTTAATTTTATTTCCTTAGTATATACAAATAAGTATATTGCAACTGAAAATTTAGAAAAAACAGAAAATAGATATGAACAAGACCTTGTTGTTAAACAAAATAACAATGTCCAAAATTACCCTCTAAACATTTACTCATATTATAGAGACATGACATTATATAGTGGGTTTTATAAATTTAACAAGCATGCAGATATAACACCAATTTGCGAATTTAAAAATCTTCATAATGACCCAGAATCTTTGATAATATCAGCTACATTTGATTTGATTTCATATACAGCCAGAGACGCAATTGCAATTGAATATACACATGGTTACGCCGTTGAAGTACCATATGTGGCATATGATGAATTTAGAGAACATAAAGCAATAATTGTTGCTCCTTATAATATAAAAACAGAAAATGCCAAAATATTTGCAAATAGTCTTATTGCTAATAATATTTATGTAAGCGATGATCGCATAGATGAAGAATATAGCAAAATTATCCAAAAAAATAAAATTTCATTTAATTTAGAATCATTAAAGGACTTTGATGAATTTAAGAAAAATATTAAGCTTATAGATGAATTATTTAGTCAAATTCCTGAAATAAAAAAGATTCCAATACTACTTGATAATAGAGGAATGGCTTTCTTTATAAATGAGCCAAAATTATTTGACATCAATTTATTAATTAAAAAGCTTAATAATTTTTATTAAAATTATATTAATCTCTATTTTTGTAATTAAAATAAAAATAGATTACATTATAATTTAATTATTAATAATAAGCAATAAAGGAGCAATATGTTATTTGATTCAAGAACCCCACAATCTAGTGAAGGATTTAAACCAAACGTAGATAATAGCATTAAAAAACCAATTCCAACAGGTGTTGAAAAATTCTTTTTTATCTTATTTTTTATACTTACAATAGGAATTTTCTATTTTGTATATGTAGGAAGAAAAAATGAATTAATGCGTGATCAAAACGAAATTCAAAATGCAAGTTCTCTAATACAAGCAGCAGAAAAAAGGAGACGTGCAGTTCTAATTAAAATGATGGATAGTTTAATTGGCTATAAAAATTTTGAAAATGAAACATTAACTAAAATAACTCAATATAGATCAAAATTATCAAATATCGATGTAGACAAAACTTCTCCCGTTGAATTGAAATCACAAATTGACAGCATCAGAGGTGCTTTGAACTTTCAATTTGAACAATACCCAGATCTTAAAGCAAGCAAATTGTATTTACAATTTTCAACCGAAATTTCTATGCAAGAAGATGAAATTTATGCAACAATAAGAAACTATAATATGATTGCAACATCATTTAATTCAAAAATATATACATTTTGAACAAATTGTGTTGCTCAAAAATTAGATTTGTACAATGTGGCTATTTTTCAAGCATCCGAAATAGAAAGAGCAGATGTAGATACAAGCGAACTTAGAAACTAAATGAGCCGAAAGGCTTATTTTTTTGCTTTATTTTGCTATAATATAATATATTAATTAATTAATATATTACAACATGCGAGGAAACATGGACAAAATAGAAGAAATACATAAATATAATGCCGATAATATTCAAATATTAGAAGGTTTAGAAGCCGTAAGAAAAAGACCCGGCATGTACATAGGCTCAATAGGGTTCAAGGGTTTGCACCACTTGCTATGAGAAATAGTGGATAACTCAGTCGATGAAGCAATGGCGGGTTTTGCTACTGAAATTAAAATTAAATTGTATCCAAATAATGTAATAGAAGTTGAAGACAATGGTCGTGGAATGCCCACTGGAATTTACTCTGGAACTAAAAAGTCAGCTGTCGAAACAATTTTAACCGTGCTACATGCTGGTGGTAAATTTGATGGATCAAATTATAAAGTTTCCGGAGGATTACATGGTGTTGGTGCATCAGTTGTTAATGCATTAAGTAGTGAATTTGAAGTATGAGTTAAAAGAGATGGCAAGTTACACTACCAACAATTTAGAAATGGTGGAATTCCTGTTAAACCTTTGGAAGTAATTGGAAATGTTTCTGAAGTTGAAACAGGAACAACAATTAAATTTCACCCTGACTATACCATAATGGAAAAAGAAAATTTTGACTTTGATACAATTATTGACCACTCCAAACAAATTGCTTATTTAAACAAAGGTTTGAAAATAACCGTTGAAAATGTTGAAAAAAATATCATCAAAGTTTTTTGTTTTGAAGGTGGACTAATAGACTATGTCAAAGAACTAAACAAAGGTAAAAAATTAATAGTTCCCGAAGTTATTTATGCAGAGGGAGTTTTTAACGATAAAAACTTTACAAATGGACAAGATGTAATAGTAGAAGTTGCAATGCAATATAATGAAGCCTACACAAATAGTATTGTTTCTTATGCAAACAATATTCAAACAATTGATGGTGGAACACATGAACAAGGTTTCTATGATGCATTAGTAAGAATTTACAATAATTACGCCGAAACAAATAAACTATTTAAAACTAGCTCAGAAAAAATAACAAGAGAAGATGTTAAGGAAGGTTTAGTAGCAATCATTTCTATTAAACACACAGACCCAATTTTTGAAGGTCAAACTAAAGGAAAATTAGAAAATAAAGATGCAAGAATTGCCACAAATAAAATTCTTTCAGACTCACTAGAACGTTATTTGAATGAAAACCCAGAAATTGCAAGAGCAATAATCGAAAAGTGTCTTCTTTCGCAACACACAAGGCTTCTTGAAATAAAGGCTCGCGAAGCTTCTAGAAAAGGTAATGGTTTAGATTTAGGTAATCTTCCAGGAAAATTAGCAGACTGTTCATCGAAAAATGCAGAAATTAGAGAATTATTTATTGTCGAAGGTAATTCAGCCGGAGGTTCTGCTAAAATGGGAAGAGATCGTTCTATTCAAGCTATTTTGCCTCTACGTGGTAAGGTTATAAATGCAGAAAAAAATTCGTTTGCTTCTGTCTTGTCAAATAAAGAAATTGCAACAATGATTCACGCCTTAGGCACAGGAATAAATACAGAATTTGATATTAACAAATTAAAATATCACAAAATTATCATTATGACAGATGCCGACGTTGATGGAGCACACATTACTACTTTATTATTGACATTCTTTTACCGTTATATGAAACCTTTAATTGAATATGGATTTGTTTATTTGGCACAACCTCCACTATATAAAATAACTAGCGGAAAAAATGTTGAATATGCATATAATGATTTACAAAAAGAACAAATCATGGCAAAATTAGAAGATAAAAGAAATGTTGCTATTCAACGTTACAAAGGTCTTGGTGAAATGGACCCAGAACAACTATGAGAAACAACAATGGATCCAGAAACTAGAAAAATGCTCCAAGTTCAAATAGATGATGCAGCAATTTGTGATACAGTATTTGCTACATTAATGGGAGAAGAAATAGAACCTCGTCATGATTTTATTCAAGAAAACGCAAAATACGCAAATAATATAGATATCTAATATGCTAAAGCCTCTAACTAATCAAGGTTTCACAAACAAAGTATTTTACGATGATGAAACTAATAGATTTATAAAAATAAAATCATATGATGGATTTAACCATAAAAGCGATGCGTTCTTATTAAATAATTTAGATTTTTGTCCAAAGATATTTGTTGATAATAAAAAAGAACTTCAAACCGAATGAATTAATGGAATCACATTAAACGAAAGTCTTTTGACAGATGACATTTTAAAAACTATTGGAAAAAATTTAATCACTTTGCATAATTCAAAATTGAAATTTTATAAAGAAAATCAAATTGCTAGAAGATTTAATATTTATAGAAAAAAAATTTCTAGTTTAAATAGAAAAATTCCAATCCTAGATAAATACTATAAAAAAATTAATTTGTTTTTAAGAAATATTGACAATTCCGCCCCTGTCCATAATGACCTTTGACTATTCAATATGATAAAAGTAAATAATAAAATTTATTTTACAGATTGAGAGTATGCCACAATGGGTGATGTTCATTTTGACCTTGCCTATTTTATTGAATCAAGCAATCTAAATGAAAAACAAGAAAAAGTGTTTTTAGATGCTTATGGTGATGATTTTGAACCCAAATATTTATTTATTCACAAAATTTTGGTAAATGCCTTAATTGTGCTATGAATAAATGCCCATGAAATACCTCCATTTGATGATAGTCTATATCTAAATAGAGTTGAGAAATACATGGAACAATTAGAAAAAGAAAAAGAGTAATATGTTAAAAAGAGATTGTTGTTGTGGTTGCAAGTCAGCTGAGTTCTGCACATGCAAAAATAAAAAATCATGTATTCCTAATAATTTATATGAAAAAATTAGGAATGTTTTTGGTTATGAAGTTTTTGAAAAACTTAATAATTTAAGACCTTATTTTGACGATTTACATAGTAGCACATATATTGGAAAACTAGATGATGTTTGAGTTCAAATAAGAATTCCTTCTGACAGTAAAATAAATTATGATAACGAAACAAAACTAGTTGAAAAGTTCAAAGATTATTTTTATTACAAAGATGGATATATTATAAAAAAATGATTCCCCGGAGTAGACTTGTTTAAAGTTAAGATTGATAGTGGAATTAAAAAAGCAATATTTAACTGCGTCAAAAATTTTCAAAACCTTAATGTAGATAAGATAGAAAAATTCGATTGATTCAAATATCCTATTCAAGACGCTGAATATAAAGCATTAGTTAAAAAATATTCAAAAGAACCTTTAGTACTAAGCCATAATAATCTAAAACGTCAAAATATTCTAGTTAATAAATACGGCTTCATTAAACTAGTAGATTTCGAATATGTTGCTTTAAATAATAAATATGTAGATCCAGTAAGTCTTTATTTATTTTTGGGTATTCCAAAAGAAGATATAATTGATTTCTTTAAATTAGACCCTTCTGTATTTGATGATTTTATTTTTTTAATGAGAGTTTACAATGAGGCAATGTATTTAAATGATTATTCAAAAAATAATTCCAAATCGCTAAGCCCTTTTGATTCAAAGAGTTTATATAGCAACAAGGATTTCTTAGAATTAAATAGATTCATTGTTCAAAAAAATCATAATAACTTTGACAATAAATTAAATATTTCTAAAATTGAAAAATTTTATTTTGTTCCACTTTGTGTTTACGAGGATGAAGATAGAACCATATGAAAATGAATCAATTCTAAACAATTAAGTTCATTCAACCACCATCAAATTAAAGTTCTTGCAAAAGCAATGAGAACACTTCATGATTCTGATGTAGAATTTCCTGAATACATTTTATCAAAGAAAATTAATTGATATTTGGATCATATGGAAATAAAAACCTTATTAGAAGATTTAAAAGGCAATAAAAGAATCAATGAAATTATTAAATGAATCAAACAAATTAAACCGGATGCAAATTGTCATAATAATTTAAATTTCAATAACATATTCTTTAATAGTAGCGACAATTTATATATTATTGATTGATCAGTTGCATATAGAAACAATCGTTATTTAGACATAGCTTTCTTATTTGAAAATACTCAAATGACACCTGAATTAGAATCTTTATTTTGAAAATCTTATGGTATGATATGTCCAAAAGACTTTTATAAATATAGAATTATTGTTCACTTTACAGCATATTTATATAATAAATTATTAAACACTGATTTTAACGCTGCTAAGGTTAACACTAAAAGAATAAATGAAATATTTGAAAAGTTAAACATTAAAGACTAAGGACTAATAAATGAAAAAAGGCAAAGCAAAAGAAACAAAAACTATTGATGTCGACAAGCAAGAAAAAAAATCAATTAGAATTCGTAGAGTAAAAACCGGATTTCTATTTTCAGGAATTTTAATTCCTTTAGTTTGCGTTGTCGCAATACCACTTTCATTAAACAAGAATTATTCATTAAAGCGAACCAAATATTTTACTGACGATAGAACTTTATATTCAATTGACAAAAACACTATAAAAGAAAATAATAAAACTTATAAAATACTTACCCTAACATCTAAAAGTCTTAATGTTTCTGATATCATGAGCGAAAGCGCAAGATATATGAATATTCCACAAAAATCATTTAATAATTCCGTTACATATTCAAATAAAATCAATATTAGAACAAACACCAACTTGTTAAAAAATACAAAAATTAATGAGACAAAGGTTACAAAAATAACTAATAGTAACCAAACTATTTTTAAATTAGATTTAGTTCAACTATGAAATGAAATAAACTCAAAATCTAATAATAAAATATCTGGGTTTGATTTTTTAAGCAATATCGAATCAATAAATAATGCTATGACTTACTCTTATATAAAAAATAACAAACTCTTTTACTTTGACAATAAGCAAGGATATTCTGGATATATTATTCAAAAGTTCTTTGAAAATTTTGATGTTGAGATTCGTAAGGAGAATCTTAAAAATAAAAATGTTAATGTTTTAGAAGCTTTTATTTCTAGCACTATTGAATTAAACAAATCATTAACCGAAATCAAAAAAATCACTTTTGATACTAAAATTACATTAAAATATGAAATATAAAAACAGGCAAACGCCTGTTTTTATATTTATATATTCTTTGAATTATTTTATACCGTAGTGAATCTTTGTAACTTTTTCTAAAGTATCTAGAGGTAAGGCACTATTCTTCAATAGAACGTCAAAGAATTCTTTTATGTATTTGTGGTTTTCTTTTTCAACAAAGTCTCTTCTTGATAGTCCTAAGTGTTTACGTACTTGGTCGTATAGAGATAGCATCTTTTCTTTACCAGCATTGTATGAAATTGCTTGTCCAGCTAGATTTAGGTAACGTTTTGATTCAGACATAATGTCACCAACACCTAATGCTGAGTTTGCTGACATAAATCTTCTTACATCATTAATTGATGCATTTGCAGGTAAATCAGATTTTGCTTCAATTCCTTGTCCATGGTATGCAGGGTCAACTGCTCTACGCATATTACGTAATTGTGCTTCGTTTAATGCTCCAAAGTATTGTAACATGTTTGTTAATTTTACTGCTCTTTGTGCATGTTCTTTTTCATCAGTTTCGCCATCTTTAGTAGTTAATGTTCAATATACTCCACCATGTAATTCTTTGATTTGTTTTATCATGTCGTCTGTAATATCTGCTTTTGTTTTTGCTGTAAAGAAGCTTGTTATTCCTTTTGCATGGCTAAAGTCAGTTGGTTTTGCATAGTAATCGTTATCTTTGTTGTCGTAGTCAGGAGTTCCGTAGTATCCTGCTTCAATTCCAAATCATTCCATAAATAATGCTCAACCTTCAACGTATGAAGTAAAGTCAAATGCATTTGCACCTAGATTTTCATTGTTTTGACTTGCTAGGTGTTGACGTGCGTAGTAAATTTGGTTGTGGTGTCCCATAATTCCTTCGTGGTTAGCGAATGAAGTAACTGATCATTTTGGCAATGAGTAGTATGGGTCACAGTTGAAGTTAAATGCACCTTGAACAGAAGTTACATTTCCGTTTGCATCCAAAATACGTGTATCTGAGTTGTATGCTCCAACACCTTCTTGATCACGTCTTTCATATAGATATGTTACTGCTTTGTAGTCAGGAACAGTTTTACCGAAGAATGATTTTCCATTCTTGATGGTTGACTTCATGAATTGGTCATAAGCCTTAAATGCTTCTAAGGCTCCATAGTAGAATTGTTCGTTTGTGATTGAACCGTATTCTTTAGCCTTGTTTTCATCTCTTGTTAAGATGTCATAACCATAAGCACTTAAATTGGTCTTGTATTCTTTAAGAGTTGGATCTTCTGATAATGACTTCTTGTAGGTATCTGTATAGAAAGTTTTATCTTCACGACCAAAGAAGAATTGTTCTTGGTTTAATCATTTTGCAAAAGCTTGTAAATCGATATTACCTTTTGCATCACGAATTGTTAATTCAAGATTTTCAACTGGTTTTGGTCCAATACCATCGGCATCATATTTAATTGTGGTCTTTCATTCTCCACTTTCACCAGCTATTAATTTAGCAACTTCTGATGCAATGGCAGTCATGTTCTTTGAAACACCTTTTGATGTTTTGTATCCTGATTGATATACATCGTTTGGAGTGTCTTCTGATGTGGTTGCCATTTTTGTGATTTGGTCATAAATGGTCTTTCCACTTTCAGTTTCATCACCGCCAACTTGTGAGAATCCAACGCCCTTAGCTTCTAAGTCAAATTTGGTAAAACCTAAACCATAAACTGATCTTTCAATAAGTTTTCCGTCTTCTTGTTTTGAAACTGATATTGTGTTTTCTGTTTCTTTGTAAACATTTTCTTTATACAATTTAAGTTCTTTTAGATTTTGACCTAAACCATGTTTTGAAGCGTTATAGTATTCTGTAGCATAGTATTTTCAGAATTCTCTTAGATCTTTCTTGTCTGTATCTGTGTTTAAATCAATTAAATCCGAAACACTAATTTCTGTTAAAGTGTCGTCGTCTAAGAATCTATTTAATTCATCTGCAAAAAATTCTCTAAGTGCTTCTTTAACATTGTTTTTGATAACAACTTTTGATAAAACTATATCTTTTTTAACAGCTTCTTGTAATCTTGCTTCTGCTTTTGCTCAGTCTTCTTGATCTAAAATTGTTCCATAGAATGAGTTTGCAGCATAAACATTTCCTGCTCCTCATCCATATTTAGATAGAACATTACGTCCACCTGCTAGATAGTTCTTTTGAACCATTTGTCAGCTGTAAATTGTTGCATCTTTTCATGCTGTTGTAATAGCATTTAAGTTTTCTTCTTTGATTAATTCTAGCAAGTTAATGTATTTTTCATAGAAGTTAGCAATTGCTTCAACTTGTTCTCTAGTTGGTTTTTCAAAATCAACATCTTTTAATTCTTCAACATCAGCAGGATTGTTTAAAGCTGAGTCTAGTTGGTTTCTAACTTCAACGATATAATCAGCTACAATGTCATATATTTTTTGATCTTTCTTTGCTGATAAGCTTGAAACTACAACCTTAGAAAGTTCAAATTTTTCTGTAACTTTATCGTTTATAAAGGCTTTAGTTTCTAGAATTTTACGTTCTAAAACTTTTATTTCTTTGCTAATAGCTTCCTTTTTATCTTCAGCAGCAGAAGTTAATTCATTTTCCTTAGCTGTTTTCTTAGCATTTAAGTCATTTAAATCTTTTTGATAAGCTTTAATTTGGTTTTCAACTGACTTGATGTTTAAAACACTTCTTCTTACTTCTTTATTGTGGACACCAGTTGAAATTCCTCATCCAATTAAAGGAATTCCGGCAGTTACAACTGCGGCTCCAAGAACAGCAGCGGCCACAAAAATTTTAGATTTTTTTGTCACTTTTTTATTTCCTTTCACTTAATATAAATGTATATACGTATATATTTATATTTGAGTTTTTTTATATACATATATATCTTAAAAAATTGTTGGTTATTTTAAACCTATAGAAAATTATAGAATGTATTAAATCAATGTTTAAATATATAATGTAATAATAAAATTTAGTAAAAAAAATAACCATAAATACTTTTAAAAATGAAAAATAAAAAACAGAAGATTTTCTGCTTTTTGGTTTACTTGGTATGGCGGATAGTAAGGGATTTGAACCCTTGAATGTTAAAAACATTATTAGTTTTCGAGACTAACCTCTTCAGCCTCTCGAGCAACTATCCGTTAGTTTAATATTTAGGTTTTTATTTTTTTAACTAAATTAATCTAATTATTAAAACAAAACAATTATAATAAAAATGAGTTAATATACGCAAAAATAGTAAATGTTTATTTTATTAACTTATCAAGTATATAAAATAAATTCAAATAATATTGTGAAAGTAAAATTTTATATATTTATGGTTTAACTTATTATTTATAAGTTTGGCTTAATCAACTTGCAATATTCATTATTGCAAATAATAATATAAAAGAGGAAAAATGAAAAAAATAATTCTTACAATTATCGATGGACTTGGTCTAAGAAAAGAAAGGCAAGGAAATGCTTATTTACAAGCAAAACACCCTTGCTTTGACTATCTTTTTTCAATGTGCCCTAATTCTGTTTTACAAGCATCTGGTCAATACGTTGGTTTGCCAGAGGGGCAAATTGGCAATAGCGAAGTAGGACATTTAAATATTGGCGCAGGTAGAGTTGTATATACGGGATTATCTTTAATTAATAAGGCAATTGAAAATGATACTTTTAAGGATAATGAAATTTTAAATGATGTCATAGATAAAACTATTAAAAATAATACAACATTGCATGTAATGGGTTTATTAAGCAACGGCGGTGTTCATTCATTAGATTTACATTTGTTTGAAATTCTAAAATTAGCGCATAGTAAAGGATTAAAAAACGTAAGTGTTCATGTGTTTGGTGATGGAAGAGATGTAAAACCACAATCAATAAAAAATTCTCTTGAAACCTTAAAAGATTTATGCCAAAAATTTGGATATAAAATCTCTTCTATATCTGGCAGGTTTTATGCAATGGATAGAGATAGTATATTTTCAAGAAATCAAGAAGCATATGATGCCATTTTAGGGCAATCAAAAAATGTTATTGAAAACATTGATGACTACATTGAAAGTCAATATAAAAAAGGAATATTTGACGAATTTTTTGAGCCTGCTCAATTAAAAGACGGCGTATTTGTTAAAAATGGAGATTCGATTATATTCTTTAACTTTAGACCAGATAGAGCAAGACAGCTAAGCCATATGTTTATTGGATCAAACTTATACACATACAAACCAAGAAACCAAGTACAAATTGATAATTTTGTTTCATTGATGAAATATGAAGGTATAAATAGTAAAATAGCCTTTAAAGAAATGGAAGTTGTAAATCCACTAGGCAAGGTTTTAGAAAATAATGACATTAAACAATTAAGACTTGCAGAAACTCAAAAATATGCCCATGTAACATTCTTTTTTGATGGAGGGGTTGACATCGAATATAAAAATGAAAATAGAATTCTAGTTGATAGTCTTAAGGTTGATTCATTTGCTGATTATCCACATATGTCGGCTAAAGAAATTACAGATTCACTATTGAACAACATTGAAAAAAATGATTTTATAATAATGAATTATGCAAATCCTGATATGGTGGGCCATACCGGTAATTTGAACGCAACAATTGAAGCAATTGAATTTTTAGATTCACAATTTCAAAGAATATTAGAATATGTGTCATTAAATCATGAAAATGTAACCTGATTTATTACAGCAGATCACGGCAATGCTGAAATTACAGAAGATGAAAATAATAAACCAGCAACAAAACATACAACAAGCCCAGTAATGTTTATTTGCACAGATAAAAATGTTAATTTAGAAAATGGTAGTCTTTGTGATGTAGCTCCTACAATATTAGATTATTTAAAAATCAACAAACCAAAAGAAATGACTGGAAAATCATTACTTAAATAATAAAAAAATAGATTCATTATTCGTTTGAATCTATTTTTTTATTATTCATTTTCTTCACTAAAAGATGAATGATATAGCTTTTCATAAAAACTACCTTTAGTCAATAGTTCTTTGTGTGTACCTTGTTCAATAATTTTTCCATCTTTAACAACTAAAATTTGGCTAGCATTTACTATAGTTGAAAGTCTATGAGCAATAACAAATGAAGTTTTTCCTTTCATTAACTCTAACATTGCATCTTGAATATTCTTTTCTGTTCTTGTATCTACATTAGATGTTGCTTCGTCTAATATCAAAATATTTTTATTAGCAATTATTGCTCTTGTGATTGCAAGCATCTGTCTTTCACCTTGTGAAAAATTGCTTCCGCCTTCTTCAACTATTTCTTCGTAACCATGTTCAAGTTGTCTTATAAAATTATCGGCCTTTGTAATTTTGGCAGCATTAATGATTTCTTCATTGCTTGCACTAGGATTAGCATATCTTAGATTTTCTAAAATTGTTGTTTTAAATAGAAATGTATCTTGCAATACTATTGAAATTTGATTCCTTCAACTAGATTCATTTATTTCGCTTGATTTATAATCATCAATTTTTATTTCCCCTTCAATAGGCAAATAAAATTTGCTTAGTAAATTTATTATTGTAGTTTTGCCTGCCCCGGTAGGTCCTACAATAGCAAATACTTGACCTTTTTTTGCTTTGAATGAAATATTTTTCAATTGTAAATTATTTAAAGAATTATCATATGAAAAACTTACATTTTTAAATTCAATATCACCATTCACATTTGATATTGTAGCAAGTTTGCTTTCATCAACGGCCGGATGCATTCTAATTAAATTTAATACTCTTGTTGTTGATATTGTTGCTGTTTGAATCATTGAAATATTTTCAAAATTTTTAATTATTTGACTAGAAGTAATTTTTATATACAAAATAAATGATGTCAATGTACCGACTGTAAAATTACTATTTGAGCCAGTGCCAATTCCAGACAATGCAAATGCTCCGCCAACAAAAATTATTATTAATTGCATTATGAATGTTATTGCATTTCCATATGGATAAACTATTCCGGCATATATATTTGCTTTTAATGAAGGTTTATATAATTTTTCATTATATAAATCAAATTGTTCGTTAATAATTTGTTGACCATTCAATGAATTTATTAATTGGTGTGCCGCTAAATTTTCTTCAACAAAACCATTCAATAAACCTATATTTCTTTGAGTCTTGCCAAATAATGGAATTGCTTTTTTAATTAGAACAAAAGCCAATATCAACAAAACAGGAAATAAGGCAATATATATAAGTGTTAAATATGTAGAATAGTAAAACATAAATCCAAGCGATGTTAAAGCACTAAATATCGCTGCTAATAACGAGTTTAAGGTTCCACATATACCATATGAAACATTATCAATATCATTTGACAATATTGACATTAATTCTCCAGAGCTAACAGTATCAAAAAATTGGATTGGCATTTTCATTATTGATTTATAAGCCTTACTCCGCATTATTTCTGAGCCATAAATTCCTGCCTTGACTGCCATTGCTGAACATATAAATGAAAAGACTTTTTCTAAAATATAGCCTAAGATCAATATGCCTATTGCAATACCAAATTCAAATAAACCATTAGAATTACTATTTCAAAACTTAAAGTTTAAATAATGGTCAATAATATAGCCCAAACCATATGTGGCAGCAGTTAAACACAATGAAGAAATTAATGTAATTATTATTATTAAAAAAACATATCCACGTTTGTAATTTAAATATTTAATTAGTTCTTTAAAACTTTCTGATTTTATTGCGTGTTGTTTTTTTCGAAATGCTTTTCTTTCAAATTTAGATACTTTTTTTAAATCAATATCAACTTTTAGCGGATCAAGAATATCTCTATTCATTATTACCCCCTAACTGTGAATATGCAATTTCTTTATATAGACCATCTCTAGAAATTAATTCCTCATGATTGCCACGATCAATAATTTTTCCTTGATTTAATACTAAAATTTCATCTGCATCAATAATTGCTGAAATTTTTTGGGCAATAATAACTAATGATATTTTTTCGTATTTAAATTCTTCTTTAATATTGTTTCTTACCATAGCTTCTGTTCTAGCATCCAATGCTGAAGTAGAATCATCTAAAATCAATATTTTAGGTCGTTTAATCAAACCTTGGGCAATAGATAATCTTTGTTTTTGACCACCTGATAAATTTGCACCCTTTTGTTCAACAACATGTTCAAATTTTTCTTTAAACTTATTGATATATTCAAAAGCGCAACCATTTTTTGCTGCTAATTCAATCTCTTCATCAGTGGCATTAGGTTTAGCAAGCAATAAATTGCTTTTTATTGTGCCACTTAATAAGCAAGGGTTTTGATAAACGTGGGAGATATTTTTTCTCAATGAGTCTGTATCAATTTCTTGAATATTATGCCCATCAATTGTTACTAGCCCGTCTTGAGTTTTAAAATCTAAATTTAACAATTTTGCAATAGTTGATTTTCCAGAACCAGTAGGGCCAATTATGCCTAAAACCTTACCAGGCTTTAAACTAAATGAAATATCTTCTAAAACATTAGATTTGGCAGATTCATAATATTTAAAAGATACATGGCTAAATTCAATTTCTCCATTAACAATTAGCTTATCAGATTTGATTTTTGCAATATCTGGCTTTTTATTCATAATTTCAAAAATACGTTTTGAGGATACATTTGCCTTGAAAATATTAAATGTAGTAAATGATGAAGCAACAACTCCAAATGTAACAGTCATAACGTAATTCATAAATGTTACCCCATTGCCTATTGATTTTGAGAAATCAGCAATATTTTCCTTGCTAGTTTGCTTTCCTACAAAAAATACAATGAAAGCAATTGCTATATTTGAAATGATTTCAATAAAATTAAATGTTATAGTGAATATTATTCATGATTTGCTTGTTATGGTTCCTCAATTTTTATTAACATTTTGGAATTTATTTCATTGAATTTGTTCCATGTTATATGATTTAATAACCTTGGCGCCTAAAATATTTTCTCTACTTTCAATGTTAATTTTGTCATACATTTCTTGTTCTTTTTTTTGAATTGGATTTCAAATGACACCCGAAATTACCATAGTTAAAGTTAATAGTGGAATAACGACTAGAAATATCATTGACAATTGAAGATTTGTTAATAGGGCAAATACTAAACCTAAAACTAGATTAAACGGCCCTATAATTAGCATCCTCAAAAAAGACATTAGTCCGGTTTGAATTCTACTAATGTCTATTGTAAATCTCGTTAGGATCTTGGCATTTGAGAAAGCCTCAATATCTTTTTGAGAAAGTTCGCCAAGATGCTTTCATAAACAACTTCTTATTTGTTTTGATCCTGATTGAGACACATATGATGCAAGCAATGAAGCACACATTGCTGAAATAGTTCCTATTATTAAAAACAATGCAAAATATCCTCCCAAATGCATTAACGCACTCTGGTGGCTTTGACATAAAAAATTAATTCTTAGAATTTCAATTGTTATTGGTCTGTTTGTTGACAAATTATTTTGAATCAAATATGCATTTTCACCAATTAACCTTGTCAATTGTCCTAATAATATTGGCAATACTAAAAATGAAACTACTTGTAGCAATGTTAAAGATATAGTTAAAAATGACAAACATTTGAATTTGTTGGCCATTAATTTATAAAGTTTAAACATTGTTTCCTCTTTCTTTTTAAATTTGTTTTAATCATTTAATTTAAAAAGTTACCTTGTATTATCATCAAAAAATTGTTTAACTTCTTTTGCGATATAAAGTTCTTCATCTGCCCTTATTATATAAATAGGAATTGAAGAGTTTTTTGTTGAAATTAATTTGTATGAATCAAAATCTCTTCCTTTATTTAGTTCACTATCAATTTCTAATTTAACTAAATGAATTTTATCAATAACTGCTTGGCGAATATATTCATCATTTTCCCCAACACCCGCCGTAAAAACAATAGCATCGATTTTTCCTTCAATCTTGTTCATATAATTAATTAAATAATCTGCTATTTTTCTTACATATAAATCAATTGCAAATTTTGCTCTTTCATTTGTTTTTTTAGCTTCTTCAACGTCACGAATATCAGAAGAAATTTTTGAAACTCCCAATAATCCTGATTCTTCATTTAACATTTTTATTGTTCTTTCAATATCTCAATTTTTATGTTTCATAATATATGGAATAATTGAACAATCAACATCACCAGAACGAGTGCCCATCATAACTCCCGCTAGTGGTGTAAATCCCATTGAGGTATCAATTGATTTACTATCTTGAATGGCTGCTAAACTTGCGCCATTTCCGATATGTAAATTAACAAAATTAACTTTGTCTTTATTTAAAATTTCTTGCAATTTTAATGTAATAAAGTGGTGATTCAAGCCATGGAAACCATATTTTCTAACATTTAATTCATCGGTTATATTTGCATCAATTGCATAAGTATATGCTTCTTTTGGAAGGGTTTTATGGAATGAAGTATCAAAGTGGAATGTCATTTTAGCAAATGGTAAAATTTCTTTAAAGGCTTTTACAGCTTCAAGAGCACCAGGATTATGAACTGGCGCTAATTCAATAGCATCTTCTAAATATGCAATAGTTTGAGGGATAACTTTTGAAGTATCTTGTAGATGTATTCCACCATTAACAATTCTAAAAGCAACGTATTTAATTTCATTAAAATCTGAAATAATATTCTTATTAATTCATAAATTAACCAATTGTTTAACTGTTTCTAAGAAACTGGGTAATTCAATACTTACTTCTTCTTTTTTGTTATTGTAATTTAAAATAATAGAACTATTTTTAATTTTAATTCTTTGTGCAATTCCTTTGGCAATAATATTTAGATCTTTTGAATATAAGGTTCATTTAATTGAACTTGAACCTGCATTAATAATTAAAATTTTTGACATAATAGCTCCTTTACAATTTATAAAATTATATTAAAAAAAATATCTTTTAAACTTAATATGATTAAAAGATATTAAAAAAGATATAAAAAAAAGCAACGTCCTATTTTCCCATTGAGGTATCGTCGGCACTGAAGGGCTTAACTGCTGAGTTCGGAATGGTTTCAGGTGGACCCCTTCGTTATGGTTGCTACGTGTATTATTATATACTATTTTTAAATATGTAATAAAAAATTTTTATTTTTTTTATTACTTTTTAAACACTCTTATTTTTGCAAGACTATTACCAATATTTTTTCAATAATATCCTTCTTTGTCTAATAAGTCTTCTGTTATTTGGCGAATAATCCCACTTCCAATGCCTACAATAATATCAAAGTAATTATTATATTCATCTTGTTTAAATTCATACAAAGCGTTTAAAATTGCTGCTGTTGCAGAGTTTACGTCATAGCCATGAAGATCTAACACCATTGGAAAAAAATTGAACTTCATTTAATATACCTTCTGACAATTAGGGCAAACATATGTACCACGTTGGTTAACTTTTAATTTAAGTGTTTTTTGACCACAAACCTTACATTTTTTTATAGTATCACTATGAATATTTAAAAAGTCTTGATATTTTCCTTCTTGTTTATTTAATGATTCATAAGAAAATAACGTTGTTCCACCTAATTCATAACTTTTTTCCATTATTTCATTTGCATTTTTTATTATGTCTTTAAGTTGCTCTAGTGATAAATCAGAAGCTTTTGAAAGAGGATGAATTTTGGAAGCAAACAATGCTTCATCAGCGTAAATATTTCCTATACCAGCAACAAAACTCTGATCAAGTAATTTTGTTTTAATGGGCATTTTTGAATTTTTTATTTTTTTAAATAATGCTTCAATGTCAATATCCTTTGGCTCAGGTCCAAGCAAAGAAATTGGAGGAATTTTATTATAATTTTCTAAGTTTCTTAGCATATATGTGCCAAATAAACGTTTATCTAAAAAGCGCAATTCGCTGTTGTCGCTAAAAACAAATCTAGCAACTAAGTGCTTATCAATTTCTTTTGGGGGGTTTAAATAATAACGATATTTTCCTTCCATTCTTAAATGAGAAAGTAAAACTAATTTTTCTGATAAAAAGAAAATTATATGTTTGCCAATGTTATCTATTTTTTCAATTTTTTTATCTCTTAAAATTGAAATGAAATATTGAGGGCTAAACTCTTTAAAAATTTTGGGTTTGTATATAATCAAATTTGTTATTTTTTTATTTAATATAGTATTATTAAGGGCTTTAACAACAACCCTAACTTCAGGTAATTCAGGCATAGTAAAATTATAATACTTTCGAATGACATTACTCATATGATTAACTGCAAAAATAAAAGTGTTATATAATTTAAATTATGATAAATAAATATCAAAAATTAATTATTTCAACTACAGACACAGTTTTAGGAATTGGTGGCCCTGTGTCAAAAGAAGTTGAAGACCTTATTTATGAAATAAAAGGTAGAGACCGCAATAAAAAATTAATAATATTAGTAAGTTCAATAAAACAAGCAAGGCAATTTAAGGAATGAAATTCACAAGCAGATGAATTTGCTAAAAAATATTGGCCTGGTGCAACTACCATTGTCGTAAATAATCAAGGTTTTAGAATGCCAAACCAAAAAAAATTGCTTGAATATATAGAAGTAAATGGTCCAATTTATATGACAAGTTGTAACTTGTCAAATGCACCTGTATGTAAAACTATTGAAAATGCAAAGCAATTATTCCCCGAAGTTACTAATGTATATAATTTTGGTCCTATGACTCAAAAACCTAGTCAAATAATTAGAGTAGAAGATGGAGAAATTTTAAGAAAATAATATGAAAACGACAGTTAAAATAACAAGCGATCACGCAGGATATGACGCAAAAATAAACCTTGCAAAAAGATTGGAAAATGAAGGCTACAATGTAGAATTATTTGGCTCAAAAACAAATAACAATAGTGATTCTTATGCGGATGCTGCTATTGAATTAGTTAATGAAATTCACAAGGATGGCAATCCTGATGAAAATAAAAATGTAAAATATGTTGCATTTTGTGGATCAGGTATTGGTATTGAAATGACTTTAAACAAATTTAAAGGCATAAGAGCAGCAAGAGTTACAAATGAAAACGAATCTCACCTAGCAAAATTGCATAATAATGCTAACGTACTATGTATGGGTGGAAGATTGTTAGAAGGTAATGAAATTGAAAAAATTTTTCATAACTGAGAAAACACCGAATTTGAAGGTGGCAGACATCTTGAAAGAATTAAAAAACTAAGCTCAATTGGTGAAAACTAATAAATTTATAACAAAAAATAGGCTCATTTTATTGAGTCTATTTTTGTTTTTATATATATTTTATTTTTCAATCTTAGAAGTATTAAATCTTCCAGATACGTTTTCTACTTTTGATGAAGTTATTCATGCATTACTATCGGCAGCCAAAATTTCTTTCTTAATCATATTTTGTTCCAAGAACAAACAATATGTTTCAATTTTAACTGTTTCTGTATTGGTATAACCTCCCAACATACGATCAATAGTGTAAGCGTGTCAATAGTTAATTTCTTTGAATTTGTTTAAAATTAAATCGGGATTCTTGCTGTAAATTTCTAGTTTTACTTTTCTGTATTTTGGATAAATTGATGCCATGATAAATGTATATAAGAATATATATACAAATGTACAAATAATTCTTAATAATAATGCTGCATGGTTTATTGGATTTCCATTTTGAGCATGTGATATTACCCCGGTAAGTTCGCAAATTGAAATAACAACTATTGAGAAAACTGCAAAGCAAATTGCGACTATTAAAGAAATTAAGCCTGGTTCTTTTTGTTTAGTTCTTGAAATGTAGTAGAGAACAAAGTCTCCCCCTGCTGTTGAAGCAGAATTTTTTCAAGCAATTCCCCCTGCACATCCTCCACATAATGCTCCAAGTACTGCAAAAATGATAATTGGTCAAGTAGGATTGTTATAACCATGTATTAAATGTTGATAGAAATATCAATAAGACATTGTTTCTTGGTAGCTTAGTTGCACGCCGTTTAGAAGTATTCCATTATTTCAAGAAGGATTAAATCCAGTACTTTTTATAGTATTTCAAACGTTTGAATAATGTGTTAGATAGTTTCCATAGACATCCCATGGTATTAATACATTTAGAGAGTTAGCAACTTCGCCATGTGCTGGAGCTAATGTTGCAGAATGAGGAGCATACCAGTTTACATAATAAAACGTTATTTGACTAAAGAAGTGGCCAACTTGTGGAATTAACAATATTGATTGAAAGATAACTTGTCAAAGTAACCAATAAGTTGTTAATATCATAAATAGTCTAGAATTTTTCTTTCAAAATGCAATTACAAAAGGCAAATTCATTGCTATATAAATATAATCAAAATATGGTGCAGTTTGAGTTAATGTTAGAGTAATGGCTTGAACCAATGCAGATAACCCAGTTGCAACAGTGGCGGCCTTAGCCAAAAATGTTGCAACACCAATATTGTATAAAACGGCTGAAATAATAATCATGACCATTTTTTTAGGAAATTTCTTTCAAATATTAAAGAAATTTACCCCATAAGGATTTATGTCAATTTTGCCGACTGATCCCTTTTCTTTTTTGGTTTCTTTCTTTAAACGTTTACGTTCTTTAATCTCAGCAAGTTCCTCAGCTGTATGTTTCATTTTCACCTCCTTTTTATTATCGTGTATATTCTAACACTTTTTAAGTGTTTATATTTTTTATTAATTATTTTTTTTAATAAAAAATACTTTTATATGACTTTAATAATTAATCTACGTATGAGGTATCAAATGAACCAATTAATCCATGAATATTTGAGATACTTATTCAGGCTTTTTTATCAACGCTTCTAACTTCTTCTAAAATTGATTCTTTTTCTAAATATAGGGCAATTGTTTCAATTTTTCCAAAATCTTGATGGTTATATCCGCCAAATCCGTTGAATACATTAAATGAGTGAACATATCCAATTTGTTTGAATCTTTCAGCAACTAGTTCTGGTTTTGTGGTGTAAATATCAATTTTTACTTTCTTGTATTTTGGATAGAATTTATTTACCAACATTGAAAATACTAATAAATAAATTGCAGTGCCCATAATTCTTACTAAAAATTCTGAAAAATCTCATGTATGTCCAATAATGTTTCTTGTATATTCTAATGAACCTATTACAATTGTTGAACATGATGAAAAGCAAATTGCAACCATTAAAAATACTGTTCCTACTGGTTTTTTATTTTTATATGCAATGTAATTAGAAATAACAGATGTACCGCCAATTGAACCACGTTGTTTTCAGGCAACTATTGATGCAAGGCCTTCCATTATTCCCCCAAGAATTGCATATATCAAAATAGGTCAATTTTGGCCATTATATATTCCATAAATTTTAGTATTTTTACCACCTACTAAATCAATACCTGTTAATAGACTAGGGATATTAGCAAGTGATAAAGAATTAGATTTTTCAAAAATTAATTTTGCATTATCAAATACCATTTTTAAATCATTAGTTTTAAATAAGTTGGCTAGATACTTAGCAATAGCTAAATCATCTCTGGACAATGAACGGCCAATTCAAGCTGTTTTAGTTGATATACTTAAAGCGTAGTTAAAAATATCTTGATAATTTTCTTTATTAATATAATTTAATACTCTTGTATCAAAACCATATGGATCTCAATAAAATTTGTATGTTAATCCGTTATTTGTAAAAGTTTGTCCTATTGGATCATAGAGATTAATTCTTTGATTAATGAAGTTAACAATAGGATTTGATGCTCTTCCTTGGTATTCAATAAATATACATTGTACAATTAATTGGAAAAACATTCAATAATAAGTTATTACCATAAACGTTCTAGGATTTAACCGTCAATAAATAAACATTAAAGGTAGGTTAACTAATACATAAAATAAACCATAAAATTGTGCAGTTGCAGGCGCTGTAAAAGTAATTATTTGAGATAAAGATGAAGTTCCTGAAGCTATTGTTGCTGCTTTTTTTAAAAATACTGTAATTGCAATATTATATAAAAATGCACTTACAAGCATTCAAAACAACTTTTTAGGATATTTTTTTCAGACATTTATTAAATTTATTTTTTGTGGATTTAGAATTTTCATTTTTTCTAAATACCGTTGGTGTTTTTGCTCTTTAGTAAGAATGATTTTATTGATTTTAGTTATTTTTTTATCTTCTAAACTCATGTTACCCCTTTCTTTAGATTCATTTAAACTATTAAATTATTAATTTTTCTTTTTTTCATAAATAAACCTGTTGCATTCTGGTTGAAATAATAGTTGTACAATTCCAATTGCCCCATGTCTATTTTTTGCTACAATGACATTGGTTAAAGAAGAACTGCCATTGACAATTTCACTTTTGTCAACAGCTTCTGCATTATCATTGTCATCATTAATGTTGTGCTTATTATAATAATCTTTTCTATGTAAAAAAATAACCATATCGGCATCTTGTTCAATTGTTCCTGATTCTCTTAAATCTGACAAAATAGGAGTTTTATCTTCCCTTTTTTCAACATTTCTACTTAACTGGCTTAATGCTAAAACAGGACATTCTAATTCTCTAGCAAGTTGCTTTAAGGATCTCGAAATCTTGCTTACTTCCAATTGACGATTTTCCATACTTGCTTTTGAAGAATCTGACATTAATTGTAGATAATCAACTACAACTAGATCAACTTTATTGTTTCTAGCAAATCGTTTTGCAATGGTTGTTATATCCGAAATTGTTGATGTAGGTTTATCATTTAAATACATTTTTCATTGTTTCATTTCTGAAACAGTTCAGGCTAGATTTTTAGCGTTTTCTGTTGAGATTTCATTTTTTCTCATTAAACTAATCGGAACCAAGGAATCAATTGACATCATTCTTTGAACAATTTGTGAATTTGACATTTCAAGAGAAAAAAATAGCACCGTTTTATTGTTTTTAGCAACATTATTTGCTATATTTAAAGCAAAAGCAGTTTTTCCCATTGCTGGGCGAGCTGCCAAAATTATCAAATCGCCGGGATTGGCGCCTAAAATTAATTCATCCAATGGTGGAAATCCAAAAGAAAGGCCAACGCCAATATCTGATTTTTCTTTGCTCATTATTCTATTAAATAGTTCATCTGCTTTATTTCCTACTTGTTCATAAGCAGTATTTATTTCTGAAATATCTAGGTTGATTAAATTCAATTGTAAATTGGATAATAAATCTGAAATTTCATAAGGCTTGTTTTCCATAATATTTATTGAACTATTTAATAAATCTCGTAAGCGATCCTTCTTGTATTGATCAATTATTTTCTTTGCATATGACTCTAATTCGGCGCTAAAGCCCGCATTTTTAGTAAGTCAGCTAATATATTCACTACCATTATAGCCAAACGAACCAATGTGATCAATTAATTTTTGGTCATTTAAATAACTCAATAAGTTGGCTATATCGAATTGTTTAGAGGCAATATGAACTTCTGAAATAGATTTAAAAAGAATTTGATTATTTTTAAAATGAAACATTTCAGGTTTTAAAATTTCAGCAATTGTTAAATAACAATTTGGTTCCCTAATTAATAAACCTAATAACGATGTTTCATTATTAATAATTGCTAATTCTCTTTCTTCTTGAATGTTCATTTTTGGCCACCTTATATTTATTTTGATTATTTCTCTTCTATGATATTTATTTTTAGTGTTGCAGTTACTTGATCATGTAATTTAATTTTTACATCATGCAACCCAAAAGTATTATATGTTACCCCTGATTCAAATGCATGGCTTGGGATTTTAATGTTGTTTTTTATTAATTCTTTTTCAATTGCTTTTGTTGTAACGTTTCCGTGCACTATATTTCCATTAGATTTTAAATAGAATTTTAAAACTAATGATTCAATTAATTGTTTGATTTCATTAGCCTTGGCTATTTGATTTGCTAAATCTTCTGCAATATTTGATTTAACTCTATCTAAATTTGCCAATGTTTGTTTGTTAATTGGTTGAGCATAACCATTTTTAATTAAAAAGTTTTTTGCAAAACCGTCTGTAACTTCAATGATTTCATTTTTTTTATGTTTTTGGTATTCTTTAATTAATATTACTTTCATTATCTTTCACACTCACTATTGCTTGTATTATATTATCAACAAATAAATTCAAATTTTCAGTTGTTTCAGCAGCGGCTGCCCCAAAATGGCCACCGCCGTTAACTGCTTCTGCTATAATTTGAACATTTGTATTAATTCCTCTTGCTGACATTTTGTATGTTTTTGTTCCTGGTAATTTAGCAACAACAAATGCAGCTTTCCTACCATTAACTCTTAATATTTCATCTGACGCTATTGAAATAATATCTGTTGATGCTTCAATATCTTTGTATGCCAAGAAATATCCTTTTTTAATTTCTTGCAAATTGGCTAATAATTCTTGAACAATCTTAAATACTTTTTCATCGAATTTTAATGTATCAACAGATTTATCATTTTGTGCTCCTCATTTTTGCAATATTGATGCTGCTGCAAAAGTTTTAGCACTTGTTTGCTTTTTAAATGTATTAGTATCTAAATAAATTCCATCTAATAAATATTGCGCTGTATCATTATTAATTTTATCTTCTTCGTGTAACAATGTGATGATTTCTGTTAATATTTCAGAAGCAGATGACGCCGAGGAATCAATATATACATTTTCTCTATATGCTATTTCATCAGTATTTTTATTTATTCTATGATGATCAATTATCACGATATTTTCTTTTTTTATGTTTTTAAAGACATTAGCATTTTCAATTCTAGTATCATCTGAAGTATCGCAAATTACAACCAATGTTTTTTCATTGTTGATGCTTTCTGCTTCTTTAGGACTAATAAAATATTCTTGAGTTTCCTTCGACATTTTATCAATAACTCTTTGAGTAGTACTATCAAATGTCTTGTTTTGAATATAAGCATTCTTTTTATAGGCTAATGCTAATTGCAAAATTGCATATGTTGAACCCAAAGCATCAAGGTCTGCATTCTTATGGCCATAGGCTATAACATTTTCAATATCTTTTGATTTTATCTTTTCTAATAAGTTTTGTGCTATATAGTTTACATTTGTCCTTGATAGATTTACATCAATTTCCGAGCTTGAGCCATAGTGTCTAGCTTTTTCATTTTTTGTTAAAACTGTAATTTGATCGCCACCCCGCATTTGAGATTGCATTAGAGCATCTTTTGCTAATTTGACTAACGTATCGTATTTAAAAATACCATAACCAAATCCACCAGAAATTGATATCAAAACATCTTTAACAATTATTTTGTTTTTTACTGAAGTTATAACATTAAAACTATCTCGTTCTAATTCTTCTAATGTTTCTTTATTTGTTATTAAAAAGAATCTTCCGTTTTCGTATTGTCTATAAATAACATTGTATTTTTTAGATATTTCATCTAATAAATTTACTACAGAAGCATAAATTTTAAAAATTTCTTCTTGGGATAAAAATGTTTGATATAAATTAATATTGTCAATGTCCAATTCCCCAAACACAATTCTTTGTTTTTTATAGTCATCTAATAAATTTTGTTGAGCAGTAACATCTCTTAAAATAACTAGATTTTTTTCAAATGATATATGAACTTCGTAATTGCATCCGTCTATTTCATACATAAATTCTTGATTATCTGAAGTTCATTGTTTTATATTAAAAATATCTTTAATGTTTTTGTTGATTAAATTGTTATGAAATTTTATTTTTAAAGATTCTGATGCTCATATAATTTTTCCACTATTTAAAAATATCAAAATTCCCGTACCAAATTGATTAAACTCACTTTGTAAATAAAATTCCAATGATTTTTTAGCTAATATTGAGTTTTTAAAATAACGTTGCAAAATAATAGTTAAATAAATTGCAAGGCCGATTAAAAATAAAGTGTAAATTGCCATTAACGCTATTTTTGACTGTTGAGTCTTTAAACAAAAAATGCCAATAAAGAATAATATTGTTGGCAAAATAAATAATAGGATAACTTGTAAAGCTATATAAATTTTTTGAATATCTTTGTTTTTCATTTTATATATATAAAATTATAATAGAAAGTTAACAATAGATTCAATTAATGATACTACAAGTCATAAAATTGCATTATTTCTTCAATACCTTGTTTTTTTTCAGATGATACCAAAAAAATATTTTCAGCTGTTTTTAAAGACAAGGCTTGATTTTTAACGCAATAACGTTGAGATTGATTTGCCTTGTCGATTTTTGTAACAACTATATCAAATTTTATTTTAAGATCGTTAAGATATTCTATTATTTCTAAATCACTAGCGCTAAAACCAATTTTAGCATCAATTAATAGCAACACTTTTTTATTGTTTTTATCATTTTTAAAATAAAAGTCAATCATAAAAGAAATTTTATCTTGCAATTTCTTTGAAATTTTTGCATAACCATAGCCTGGTAAATCAATAATTATTTTATTTTTGTTGCTTTCAAAGTAATTAATTAATTGTGTTCTTCCTGGATAAGATGATGTTTTTGCAATATTGCAAGAAGCCAGTGAATTAATTAATGATGATTTTCCAACATTAGATCTTCCTCAAAATACATATTGTATATTGTCAGAATTAATAAAATCATCGTGGCTCTTACAACTTTTTAAGAATTTTCACATTATCTATCTCTCCTTAATAGTTCATATGCTAACCTAGCAATCATCATTGCATTATCAGTTGTATATTTCATTTCCGGAATTATGGCATTTGAATGGACATTTAAAAACATTTCTCTAATTGCTTTATTAGCACTTACTCCGCCTGCCAAAACTATTACTCTTGGATGATATTTTTTAATTGCAATTTCTAAATGATTTTTTAAATATAAAACTACTGTATTTTCAAACATTGTTGCATATTGATCAACATTGACCTCTTGTTTTCTATTGATTTGGTTATTTATAAGATTTATAACTTGTGTTTTTAATCCACTAAATGAAAAGTTCAATTGAGCTTCAGTATGTGGAATAGTAAAATGTTCTGTGTATTTATTTCTAAATTCCATTCATTTTTTATCAATAATAGGTCCTCCAGGAAAACCCAAATTTAATTTTCTTGCTATTTTATCAAATACTTCTCCGACGGCATCATCTTGAGTTTGACCTATAACTTCGTATTCATTTTTTGATTTATATAACATTAATTGTGAATGCCCACCTGAAACAATTAAACCTAAAGCAGGGTATTCAACTTCTTTGCCTATAAAAGCAGAGAAAAAATGCCCTTCTAAATGATTCAAAGGCAATATAGGTTTTTCAAGTAACATTGCTATTGCATTGGCAACAACATATCCAACATGTAAAGAACCAACTAATCCTGGTTCTTTTGTATAGGCTACATAGTCAATCTTTTTAATATCAATTATTTTTTTTAATTTTTCAATTAAAATACCTATATTTTTTACATGCAATCTTGATGCTAATTCTGGAACTGTGCCTCCATATTTTCTATGAATGTCTATTTGGCTAATTGTTTCCATTCAAATTGGTTTGTTATCTTCTAATAAAGCAAATGATGTATCATCATGAGAACTTTCTATGGCAAAAATTAACATTTTATCTCTCCTATTTGTGGATCATGCAAATAAATTAATTCACAATTTTCAATATCATTTACTTGTTTAAATTCTTTTAAATATTGCTCTATATTTTTTGAAAAAAGCTCTTTATTGAATAAATCAAAATTGCCTAAGTTTTCTTGCATATGACATTCAAATGGATCAACAATTATTGAATAAACATTGTATTTATTGGCTTTTATATTTATTAAATTGCAATCTTCATGTTGCAATTTTTTTTGTGTATAAAATAAAGAATATGTTGGGGCTACAAATAATTTGATATTTGATAATTGTGCTACTGTTTTGGCAAAAACTAAGCCAATTCTTGCACCTGAAAAACTCCCAGGTCCTAACGTTGTATAAATTTCTGAAATTGAACTTAATTTAAAATTATTTTTATTTAATAAATAATTTAAATTTTCAAAAAATGCATCGGTTTTTTTAACTAAGTTAGCAATAAAAATATAATCAATCTCGCAATAATTTTCATCAATTAAGCTTATATAAAAATCGCTTAATGAAGTTTCAATGAATAATTTCACTATTGTTCCTCCATAATTTCAAATACATGGTTTTGTTCATTGTCAAAATAAACTTTTATTTTTATATAATGTTTAAATTTGGGCAATGTCACATTTTCTGCTCATTCAATAATTGCTAATTTATCTTCAAAATAATCTTCATAAGCAAATAAATTTGTAGTTAATTTATAAGCATCAATATGTACAAATTTATCATACACTAAAATTGTATTGAATGTTGGTGATATTACAGTCTTTGGTTCGTTTAATTTTTTTGCTATTTGACTTGTTAAAGTTGTTTTGCCTGCTCCGAGTTCGCCTATTAAAAGCAAGGCTTCTAAATTTTTAAATGACAAAATATAGTCAACTAGTTTATCTAATGGTTCGTTATTTTTATAAATAAATTTTAGATTTGACATGATTTCTTAACCTTGATAATAAAATACATTATTATTTTACCATTTATGCACCTTTTTAAGGCTTGTTACAAATAAAAAATGTATTTAAAATTTAAAAAATAAACATTAAGTATTTAAAACACAAACTTAATGTTTATTTATTTTTTTAAGCAAGATGTTATTTTATTTATATTGAATATTTTCTCAATCAGATTGTTTAAAATCAGTTAAAAATTTTGTTAACAAATCAACGGCTGCCAATAAATCACTTATGCAACATACTCCGATTGGTGAATGCAAATATCTTTGAGGTAATGAAATTGTTAATGTGGCTGCTCCGCCTTTTGAAAATTGCAATTCAAAGGCATCAGTACCCCCGCCCATGGCTACATATTTAAATGTTGGAATATTATATTTCTTAGATAAATCAACAAAAAATTGTACCAATTTTGGATCCATCATTGTTCCTCTATCTTTTAATCTTAAAGCAGCTCCTTTATATAAAGAAACATTTCCAGGAATTGTATTAGGTGTATCGTGAGATGCAGAAGTATCAACTGCTATCGCAATGTCTGGATCAATCATAGACGCAACTGTTTTAGCTCCTCTTGTTCCAACTTCTTCTTGAACTGTACCTACTAAATATAGATCAATGTCAAGATCTAATTTTGCAACAGCTTTAGCAACAAATTCTAACACGGTTACACCAGCACGGTTGTCCATTGCTTTTCCACCAATTAAATTTTGATCATTAAATAGTATTGTTTCTCCACTCATGTAAACTCTATCGCCAACATTAACACCATGAGCAATAGCATCTTCATCGCTTTTGAAACCAAAATCAGCAAATAATTCATTTTCTGTAATTGCTTTCTTTCTTTCTTCTTCTTGCATTATATGAATACTTGTATGTCCAAATAATCCAATAAATGTTTCGTTATTTGAAGTAATTAATTTTGCTTTGGTTCCAATAACTGCACATGGTCATACACCACCAACTGTTGATAAATATAATTGTCCTTTTTTATCAAAATTTCTTACTACATATCCAACTTCATCCATGTGTGCTGCTAATAAAACTTTAGGAGCATTTGGATTTTTAGATGGCTTGTGCATAATTAAACAGCCAAAATTATCTCTGCTAAATTTAAATCCTTCATTAGCTAATTCTTTTTTTAATTCATTAACAACTGGTTCTTCATAACGAGACATTGCTTCAATTTCCATATATTTTATTAATCTTTCTTTGAATTGATTTTCTCTTGTCATATTAATTCCTCCTTTATTTAATGTATAAGATATATTTTATAACAAATTGTATAAAAAAATAGCCATTAGGCTATTTGTTGTTCTTTGAAAACTGAATACGACTTAATTTAGATAACTATATAGAATATTACTATTAAACCAATAAATTTATTAGTACTGGTCAGCTGAATGCATTGCTGCACTTACACCTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACACCATCGGTGCGTCCACTCCGGTCCTCTCGTACTAAGAGCAGCTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGATCTCTTGGGAAAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCACCACACACTGTCCTCTTCCCGGATAACGGGAACAAGTTAGAAGTTCAATGTAACAAGGGTGGTATTTCAACGGCGACTACTCTTAAACTAGCGTTCAAGCATCAACGTCTCCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAGTCTATTGTCGAGACAGTTAAGAGATAATTACTCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCACATTAATGCTTCGCTAATGCTAACACCTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAACTTACGGGATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTGTGTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAAATCATCTAATTCGCTACTCAGTTGCCCTTTTGCTATGCGTCGTAACTCCCAGTTAAGTTATGCGGATTTGCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCCTTTCGGCCTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAAGCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAGAAACTTCACAACCTTTCTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTATCACCGATGTACCGACTGCATAGTAATACATGATAGTATTCGGAGTTTGATTATAGTCAGTACGGCTAGGCGCCGCCATTCCATATTCAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCACCCCTACCCACAAGTCATCCGGGCACTTTTTAGCGTACTGCGGTTCGGTCCTCCACTTAGTGTTACCTAAGTTTCAACCTGCTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTCACGTGCCCCGCCCTACTCAGGATACTGCTAGGAGATTTATACATTTCGCTTACGGGAATTTCACCCTCTATGTTTAAGCGTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTCTTTATTTTCTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTCCAGACCCAAGGCATCCACCAAAAACTCTTGCTTGTTTAATAGTTTTGTTAATTGTATGACCTATTGTTTGTCTTATAAAATATAAATATTCTATGGTTGATCTAAACTTTGAAATATTAATTAATATATCAATGTCGATATTCAGTTTTCAAAGAACCGAGAGATAAATCTCTCAAAACTGGATACAAATATTTTTTCCATAACGTAGGTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTCACCCCAGTCACCAGTCCTACCTTAGGCGGTCGCCTCCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCAGTTACCCAGGCAGTATCTCTAGAGTCCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGACTTGACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCACTCCGATAACCTCCACTATATCTCTATAGCTTTGCGAAGGATGTCAAGAGTGGGTAAGGTTTTCCGTGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGCCCCGGGATTTAACTCCAGACTTAACAAACAGCCTACGAACGCTTTACGCCCAATAATTCCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCATCGCTTTCTGACAAGGTACCGTCAGTCTGCAATCATTTCCTATTGCAAATGTTCTTCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGTCTCTCGACCCGGCTAAACATCATAGTCTTGGTGGGCCATTACCTCACCAACTAACTAATGTTCCGCACCCTCATCTTTTAGTGACGCCTTGCAGCGCCTTTCACAACGGAACCATGCGGTTCCATGCGTATCCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATTAAGCACGTGTTACTCACCCATTCGCCGCTAGGTTATTGCTAACCTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTTATAAAAAAATTTGAATTGGTTGATTATAAATAAAAAAATAAATTGACGTTAATGGTATTCGTATCCAGTTTTCAAAGAACTATCTCGTTGTTTCAAAACAACCTTTAAATATTATATACACTTTTTTTTATTTTTTCAAAAAAAATTAAATTTTTTAGCGATATAAAATTGCTGTTTTAAAACAGAAAAGTAAATATGTATATATTATAACTGTTTTATTTTTTAAATAATAAAAAAATCAGGATTTTTCCTGATTCTAATATCTATTCATTATTTTATTGTTAACCTACTATTTCTAGATCTGCATAATCAAATTCAGTTGGAACTTTTCTACCAAATTGTTCAATATTAACAAAGGCTTTTTGGGTAATATCATTATTTTTAATTATTTCGCCAACTTCATCTTTGAATATTCCACTAGAAATTTTTACTAAAGCCCCTTCTTTAAAAGCAGTTTCAATGTCCCCTGCTTTAAATTTAGCTAGCAACATTGCTTCTTTATCAATCATTAATTGGAATTTTTTATCACTTATTGGGGTTGGTTTTGTTTTGTTACCACTTGAACCAATTAGCCCGGTTACATATTGAGTATTTCTAACAAGATATCATGTATCATCAGTCATTGCCATATTTAAGAAAATATAACCTTTGTACATATTTACAAATTTAACAGTAAATTCTTCGTTTCTTTGCTTCTTTTCTAATTCTTTATTTGATAAATGGGGTTCTTTAAACATCCTTATATCTTTAAAGTATTCATCCATTCTTTGAGCAGCAATTTTATTCTTTAATGATTCTATAACATTTTCTTCTTTGCCTGAAACTGTTGAAATCATATATCATTTAAAATCATTATTTGTTAATTCCATATTCTTTCCTTACTAAGCCTTTATTCCTACTGAATTTCATAAACTACTAAATCCAAGGGTTATTGCAAAACAAAATGCAGCCATTAAAACTAGAAATAAAATTGTAATACCAAATCATTTTCAAGATTTTGCAGATTTTGGCCAGATAACTCTCTTTATTTCCTTAATTCAATTTCGAAATGTATATAGCTTAACTTGACCTTTTTCTGCTTTTGCTTGCAATTTAGCTTGTTTTCTTTGTTCTCTAGTCATTATTTTTCCTCTTTGTGCAATGTTTGTTGATTACAAAATTTGCAAAATTTATTAATTTCTAATCTCTTTTCATTGCTTTTATTTACCGAGTAATTTTTATGTAAACATACTTGGCACGCTAATGTAACCTTCTTCGACTTTGTATTCATAATTAGATATTATAATATATTAATTTCAAATTTTCAATCATTTTTAATAGTAATTTATATTAAATAATTGTTGCGTAAGAAATTTTATATAATTTTAAAATATGAGTAAATGAGCAATTTTGTTTTTAGTAATCTTAATTATGCTAATTGCAATGTATTTAACATTTATTTTTTATTATTTTATAAAAATATATCCCCAAGTTTTAAAATCAAAATTGGATAATTTTATTAATAAAATTTGAACATTTATTAATAAAGCAAAAAATTCTTATGACAAAATTTGTCAATTAAGTTTGAACAATGATGAATGCAAGGAACTAGAAAAACAAATTGGAATTAATTTGCATGAAGTTTGCAGTTTATATAAGGCATGTAAAATATTAAAAACCAAAGTAGATGATATTGTTTATTTGCATATGAATAAGCATACTTTTTGGCATTTAAAATGCACAATTCTTATTAAAAATAAATATAAAGAAATTTATTTTAAAAGACTAGAAATTTTTAATTTACTTGAAACCATCTTTAAAAAAGTCGCAGAAATTGATAAAAAAAGTGGAAATATACAAAAAATCGTAAATAAATACAAAGTTAGTTTAAATGATTTATCAAATATAATGGCAGGCGAACATGAACATTTTCCTCAACACTTCATTGATAATTTAAAAGACAAAAAAACAATTATACAAAAAAAATTAGTTGATTTTAATAATGATGAATATCTAAAATGAAACGAAAAAATATTAAAGCAAAATTTATTAGAAATTAAAAATTTAATCATTGAATATGCCACTTTTTATTTTAAATTTAAAAACAATTACAAGAATTTAAATTATATTGTTGAAAAATACAAAAAAAATCAACAGCTTATTAAAGAATTAAATAATATTAAACTAGAACAATTAATAAAAGAAATGTTGGAAAAAGAATTTAAATTTAATTATGAGATAGAAGTTGAAAAAATAAAAACTTTGATTAATAAATGTCAAAATTCAAAAGACATTTCAAATATTGAACAAATTATTAATAAACTATTAGCTTCGATAAATACGACTAGTATAAAAATATATAACTATATTAAAACCTTTGCTTTTCATTTAAAAATACAAAATAATATAAAAAAAATCATTGAAAAAATTGACATTCAAAATAAAAAAATTTCTGATTTCTTTGCAAGCAAAACTAAAAAGAATTTGCTAAAAAGTGAGTATTTTAATGTCTATAAGCCAATATTGAATAGCTTTATAGATATTAAAAATAATAATTGAATGGATATAGTTGAATTTATAGAGTTGAAGAGATGATTAGAATCCTTATTGAATTTGTTGAAGATTCAAAATAAAATAATAAATAAAATAGACGAACAAGAATATCAAAACAATCAAATTGAATTTTATATTGAAAACTTTAAAAGATTAACTGCCAATATAAAAGTATTAAACATTAATTTAAATCCAATAGACATTGAAAACATAAATAGTTTATTTACTTTTATTTATAAATTCAAAAATAATAATTTTTCTGAAAACGAATTGAATGACTTTTTAAGTTATTTATCTAATGAAATAAATTTTTTAAATTGCAAAATTCAATATTTTTTAATAATTAAAAATGTCGAATTTTCTTTAAGAAAAGACGTAAGTGAAAATTCAAACGCCCAAAATTTATTTAATGAGTTGCAAAAACTTAAAGGTTATAATGATGATAAAAAAGCAATCGATTTAATATATGATTATATAGAAGAACAATACAACTAATGGAGTAAAAATGGACTACAATAAAATTTTAATTAGATATGGCGAATTGACTCTTAAAGGTGAAAATAAAAAGGATTTTATTAGGCAATTGCATAACAACCTATCTTGATATATAGAAAAATCAGAAATCAAAACCGAATATGATCGGGCATTTGTTGATTATTCACAAAAGAACTTAGACTGCTTAAAATATATTTTTGGAATTTCTTCATATTCGCCTGTATATGAAACAGAAACTAGCCTAATCAATATTGAAAATATAGTAAAAGAAATAATTAGCAAAAAACAATTTGATAGTTTTGCAATAAATGCGCGAAGACACAACAAGAATTTTTCAATGACTTCAAGTGAATTAAATATGTATTTTGGGGGTATTGTTTTAAAACAAATGCCTGACAAACAAGTTAAATTAAAAAATCCTGATTTAACTATATATATTGAAATAAGAGATCAACGTAGCTTTGTTTTTTGCGATTATTTTGACGGCTTAGGTGGCATGCCGGTATTAAGTTCTGGAAAATGCATACATCTAATTAGTGGAGGTATTGATTCACCTGTAGCAGCGAATTTATTACAAAAAAGAGGCTTAAAAATAGTGTTTTTAAATTTTATTACCCCTCCTCAAACTGATGAAAAAACAATCGCAAAAATAGATGAAATTGTTAATATACTAAAAAAATATCAAGGCAATGCTATGTTATATCAAATTAACTATAGCAAAGTGATGGAATATATTGGTCTTATAAGCAATCAAAAATACAAAATAACTTTAATGAGAAGATCATTTTATAGAATAGCAAATCTTATTGCAAAAAAAGAAAACATTAAAGCAATTTCAAATGGCGAAAATTTAGCCCAAGTTGCCTCTCAAACTCTTGAATCTATTTATACTATCTCCGAAGTTTGTGATTTGCCTATTTTTAGACCTTTAATTTCTTTTGACAAAAATGAAACTATTGCTATAGCAAAAAAAATTAACACATTAGATGTTTCAATAGTAAGGGCGTGTGAAACATGTGAATTATTTGCTCCTAAAAATCCTGTAACCAAGCCTAATAGACAAGAAGCTCATAAACTTGAAGAAGAAGAATTGCCTTTGATAAAAGAATTTGAAGAAGAATGTATTAAAAATGCTATTATAAAAAAATATTCAATATAATCGTTTAAATGTTTAATAAAAAAAGGTTTGATTGAACAAACCTTTTTTATTATTTTAAATCTTTTAATTGAGGGAATAAAACTACATCTCTAATTGAATCATTTTGAGTTAGTAGCATAATTAAACGATCAATTCCTATACCAGAACCGCCTGTTGGAGGCATTCCATATTCCAAAGCATTAACAAAATCTCAATCAATTTCATTTGCTTCTTCATTACCATTTTCTTTTTCCAACAATTGGTTTTCAAAACGTTGCAATTGGTCAATTGGATCATTTAATTCAGTAAACATATTTGCAAATTCTTTTGTTCCAATAAATAGTTCAGCACGTAGTGTAAATCTTTTATCATCAGGGTCAATTGCTGCAAGAGGAGAAATTTCAATTGGGTGGCCATATACAAATGTTGGTTCTATTAATGTTTCTTCAACATATTTTTCAAATAATTCATTGATAATGTGACCATATCTAAAGAATTTTTCAATTTTAATGCCATGTTTTTTAGCTAATTCAAACATTTGTTCTTCATTTAATTTTCTAATGTCAACATTAATTTTTTCCGATATAGCATCAATCATATTGACTCTCTTAAATGGAAAATTAAATGTTATATCAAAACCATTAAATTTAACTTCTTTAATGTTGAATTTATTTATTAAATGTTTGAAAACACCTTCAGTTCTTTCCATCATTCCTCATAAATCTGAATATGCTTCATAAAATTCAATTGAGGTAAATTCTGGATTATGAGTTGTATCTACGCCTTCATTTCTAAATATTCTTCCTATTTCATAAACTCTATCCATTCCACCAACAATCAATTTTTTTAATGGCAATTCAGTTGCAATTCTTAAATAAAAGTTTCTATTAAGTGCATTATAGAAAGTAATAAAAGGTTTGGCAGCAGCTCCTCCTAATATTGGATTTAAAATTGGTGTATCAACTTCAATGTAGTTCAAACTATCAAAATATTCTCTAATGCCTTTGATAATTTGGGTTCTCATAACAAATGTATTTCTTGTTTTTTCATTCATTATTAAATCTACATAGCGTTGACGACGTCTTTCTTCTGGATCAACTAAACCATGAAATTTATCAGGTAAGGGTTTCAATGATTTTGTTAATAGAACTATGTCTTGAGCTTTGATAACCAAAGCATCAGTATTAGTTTTTGACAATGTTCCTCTTACACTTAAAATATCGCCAAAATCCATTAAAGAAAAGGTTTTTGCAGAAGACTCATTCAATGTTTTTTTGTTTGCATAAATTTGCATTTGTCCGCAATTATCTTGTAAAACAATAAATGGACCACGAATAGTCATAATCCTTCCATTAAATACAAATTCTAGATTTTGTTTGTCAAGATCTTCACGACTTAAATTTTTATATTTTTCATTTAACTCTTTTGAATTTGTTGTTGGTTTTATAGTTTTGTGAAATGCTTGTAAATTGTTTTTTTCTAATTCAGCAATTTTATTACGTCTAATTTCTTCTTGTTCACTAAGTTTTCTTTCCATAAATATTTATCACTTTCTATTTCTTAAAATATCAAAAAAATTATATATTATATGCTTTATTTTTTTTATAATTATTAATGCATAATTGCGTGGTCGCATAGCTCAGTGGAAGAGCACGAGCCTCCTAAGCCCGGGGTCGCAGGTTCAACTCCTGTTGCGATCGCCATTTTTTTATACATTTTAATTAATTAAAAATAGTCCAAAAGGACTATTGGGCATTCTTCATAGAACGCATAACTTGTCTAATTTGTGCTTCTGAAGCTTTTCTCCCCATTTGTTGAAACATTACACGAATCATTTTTTCAGTAACTGGTGGATTTTCTTTTAATTGCTTCTTGAATTTTTTTTGTGTTATGTATACCGCAATTATTCCACCGATTATGGCAGTAATTATTGGTAATATAACTAATAACGCTATTCCTCAACCTTGCATATTCTACTTTCCTTTCAATACTGTCTTCTTTAAAATAATATATATTATAAAGGGCAATACAATTAAAACTGCGATACTTATAAAGAAAATTTTGGCATAAAATATGTGAGCTTTTCTTTTACTTTCATCAAGTTCAACAAAATTCATTCCTTGTTCATTGCCAAAGAACGATTGGATGTCCACCATTCTTTTCTTTTTAAAAATTGTACTGAATATAAATAATATTAAAACTATTCCAATTATACCACATAGTACATAAATTAGTATTTCGGGTTTTGGAAAAGTATTATGAATTCATTTTTGTCAATCCAATAAACCCCATTTTGCATCTTTTAAACCTCAAAAAATACCTATAAACACACCGATATAAAACACAATAGCAACAACTGCTCATGTTTGTCTTACTTGTCTTATCATTAATTTCTTATAAAGTAAGGCAATAAATGGCGGTGTTGATGTCGCACCATTTTTAATTGTAATCCTATACATATCTATGCTTTTATGTATTCCTGAAAGTTCAATCAATTCAATAATAAATCTAGTTAACGAAATTACTATAAATATTGCCGGAATAATATATCAAGCCCATTTTATTTTAAAATCTTTTGCATTAGAAAAATTGAATAAATTTGGTCAAAAGTAAAGCAATAATATTGCTATAACGCTCAAAAGAAGGATACCAAAATTTCAAAGTATTGATATTTTTTTAATATTCATTTCTCTTTGAAAAACTGAATAAATTCCATTAGGAATTATTTTCTGAGGATCTTTTATTTTATAACTTAATTGTTGCAAATTTTGAGTATTCATTGAATCTTCTACCATGTGATTTTCAAAGGTACGTGTTGGAATTTCAATGTTTTTACTACTATCGCTATTATTTCCTGTTGTTGTGGGTTTAATATCCATTATTTTCTAATCCTTTCGAGAATCATCTGTTTTTAATATTGCCAAAAATGCCTCTTGTGGAACTTCAACGCTTCCTATTTGTTTCATCCTTTTTTTACCTATTTTTTGTTTTTTTAATAGTTTTTGTCTTCTTGTAACGTCACCACCATATAACTTAGCTGTAACATCTTTTCTATATGCCTTAATTGTTTCTCTTGCTATAATTTTAGCACCAATTGCAGCCTGAACGGGAATTTCAAAATTTTGTCTTGGTACTACTTCCTTTAGTTTTTCTGTTAATGCTCTACTTTTATTATATGCTGAATCTCTGTGAACAATCATTGCTAAGGCATCAATTTTCTCACCATTTAATAAAATATCAACCTTTACTAAATCGCTTTCTCTTAAACCAATGTAGTCATAATCAAATGAAGCATAGCCTTTTGAATAGCTCTTCATTAAATCAAAGAAATCAAATATTATTTCATTTAAAGGCAATTCATAAATCAATAATCTACGTGTTTCATCTAAATATTCAATATTTACATATTTGCCACGTTTAGATTGACATAATTCCATAATTGGGCCCAAATATTCTTCGGTTAGCATTATGTTAGCCTTAATATAAGGTTCCTCTATCATTTTTATATAACTTCTATCTGGAAATAGTGATGGATTTGTAATATATTGAACATCTCCATTAGTTAGTGTTACAACAAACTCAACTGAAGGAGCAGTAGCAATAACATCTAAATTAAATTCTCTTTCGAGTCTCTCTTGAAGAATATCCATATGCAATAAACCTAAAAAACCTATTCTAAATCCAAATCCTAGGGCTTTTGATGTCTCTGGTTGTCATACCAATGAAGAATCAGATAAACTAATTTTTTCTAGGGCATCTTTTAAATCATTGTAATCTCTAGTATCAACGGGATAAAATCCGGTATAAACAACTGGTTTTATTTTTTTATATCCTGGCAATGGTTCAGGGCATTGGTTTTCAAATAATGTGACAGTATCACCAACTTCAACATCTTTGATATCCCTTATTGATGCTGAAATTCAACCAACTTCGCCAGCTTGCAATTCATTTTTTTGAACTTCATTAGGGTTTTTAACGCCTAATTCAGTTACTAAATAATGCTTTTTTGTTTGCATAAAGTAAATTTCGTCGCCTACTTTTACTTTGCCGTCAAACAATCTCACATAGATAACAACGCCACGATAATTGTCAAAATAGCTATCAAAAATTAAAGCTTGCAATGGTTTATTTATATCTGCTTCTTTAGGAGCAGGAATTTTGTTTATAATTGCATCAATTACCTTATCAATATTTAATCCTGTCTTTGCAGAAATCATAATTGCGTCAGAAGTATCAATTCCAATCATGTCTTGAATTTCTTTTTTTACTCTTTCAGGATCTGCACTAATTAAATCTACCTTATTAATAATAGGAACGATTTCTAAATTATTTTCCATAGCCATATAAACATTAGCCAATGTTTGTGCTTGAATTCCTTGAGTTGCATCAACTAGCAACAATGCTCCTTCACATGCAGCAAGTGATCTACTAACCTCATAAGTAAAATCAACATGACCTGGTGTATCTATCAGGTGAAAAATGTAATCTTTATATTTAATTTGAATAGCATTTAATTTAATAGTGATTCCACGTTCTTGTTCTAGTTCCATTGAATCTAAAATTTGAGGTTTTGCTTCCCTGGCTGAAATTGTATTGGTAAATTCCAAAATACGATCAGCTAAAGTACTTTTACCGTGATCAATGTGTGCTATTATTGCAAAATTTCTTATTTTTTCGTTTGTCATATTATTTTAAATTATATTTAAAAATGTCATACATTTAAAGAAAAATAAAAAATAACCATAAATTAGATACATTTATTAAATATCTCTATGGTTATTTTTTTTAATTATTTTTTGTTTCTTTGGCACCAAATATTTGTTCAAATATTTCGTCATAATAATGAACTGGCACAAATTCAACAACATTTTTAACTTCAGCAGGAACATCTTTTAGATTCTTATAATTATCATATGGAATAAAAATCTTTTTAATACCAAATTGAGCAGCCGCCAATGATTTTTCTTTTAAACCACCAATTGGTAGAACTTTTCCACGCAATGTAATTTCTCCAGTCATACCGATATTAGCAGGGACTGGTCTTTTAGATAAGGCACTAATTATTGAGGTTGTAAATGTTACTCCGGCACTTGGGCCATCTTTTGGAATAGCACCTGCTGGAACGTGAATATGAATTGCTGTATTTTCAAAATCAAAGTCAATATTAAATTTGGCAGCATTTGCTCTTACATAAGCTAATGCAATTTGAGCAGATTCACGCATAACATCTTTTAGTTGCCCTGTCATTTTTAGGCCTTCTTTTCCAGGGAATGTTGTAACTTCAATAGCTAATGTTGAACCACCAAAACTTGTATATGCCAAACCATTAACAACACCTATTTGTGCCTTATTTTCATTTTCATCATCATCGAATTTAATTGGTCCTAAAAATTCAATTACAATTTCTTTTGTAACAATAAAACTATCGTTGATTTTTTTGTCAAGAATTTCGACAACAATCTTTCTTGCTATACTATCTAAAATACGTTTTAATTCACGAACTCCACTTTCCCTTGTGTAGTAACGAACTAAAAATTCTAAAACTTCGTCAGAAATTTGAAATTGTTCAGGATTTAAGGCATTTTGTTCATACACTTTTGGAAGTAAATGTGATTTAGCAATTTCTACTTTTTCTTTAGTAGTATATGTTGAAAGTTCAAGCAATTCAACACGATCAATTAGTGCTGCTGGTATTGAATCATAGTAGTTAGCAGTAGCGATGAATAATACTTTTGATAAGTCGTATTCTAAATCCAAGTAGTGATCTTGAAAATTAACATTTTGTTCAGGGTCTAACACTTCAAGCATTGCGCTTGTTGGATCGCCTCGAAAATCTGATGACATCTTATCTATTTCATCTAACAAAATGACTGGGTTTGAAACTCCAGCTTTTTTAATTGCTGATATTATTTTTCCAGGCATTGCCCCAACATAAGTTCTTCTGTGTCCTCTTATTTCGCTTTCATCCTTAACACCGCCCAATGAAACCTTTATAAATCTACGATTTAGTGATTCGGCGATTGATTTAGCAAGTGTAGTTTTACCAGTTCCTGGGGGACCAATTAATGTTAAAATAGGAATATTATTTACTGGTTTATTTGTGGTTTGACCCGTTTTATGAACGAATAATTTAGTATCAATAAATTCTTGATCATTTCCTTTTATTGGTAAATATTCAGTTTTTTCTTCCTTGATTTTAAGATGAGTTAAAACCGAAATAAATTCAAGAATTCTTTCCTTTGGCTTTTCAAGTCCATAGTGATTCTTATTCAATACTTCTCTAACTTTGTTAATATCAATTAATTCTTTACTTACTTTTCTTCAAGGCAATGCCAAGATTAAATCAATATATGTTTTAGATATATTAGCTTCTGGGCTAGATGGCATCATTGAGGCCATTCTTGATTGTTCAGATCTTAACGCTTCTAGAACATATTCAGGATATTGTTCTTTTAAAGTGCTGTCTTTTTCAATGTCGTCAGCAGATTTTCCACCTTCATCATCCTTCAACATTTTTCTAATTTGGCGCATTTTTTCTCTTAGGAAGAAATCTCTTTGTTGGTCTTCCATATCTTCACGTAAATTACTATTAATTTCGTTTTCTATTTTTTCAAATTGAACTTGCTTATTTGCCTTTTTAATTAGTTCATTGTATCTAGCTAAAACGTCATTGCTTTCAATTAAAGAAAGAAGTTCATCACTCAAAAAGTACTTTTTCAAAAACTTTATAAGTTGTCATGATTCAGTTTCATATTCAACTAATGATTGTTTAGGTTTGTTAGTTTCAAATTCTTTTTCTAAATCTCGAAATAAAACTGTTCCTATACCTTCGTTAATAACTTGTTTAGATAGTGAGAAGAACTTTTGTCTTATTTCTTCAATTTTTGGTAAATATGAATCATATGAATTGACTTGAATAGGCGAAATATCAACATAAAAATTATCCTCACTATTTGTTGATTTTTCATCAACTATTGAGATTTTATTAATATTAACCGGTTCTTTTGCTTCTAAATTAACATGATAAATTCCTGATTCTTCACTAATGTTAGTAATATTAACAAGTACGCCTACAGGAGCAATATCATTAATTGTATGTTCCTCATTTGATTGTTTATAATTTGGATTTAAAAATGTAATAACTATATTTTTATTTCCATTAAAAGCCTTTAATAAAGCTGATACTGATTTAGCTTTTCCAACTCTAATTTTGTCAGAAGTATATGGAATAATAATCTGATTTTTTATTGCTATATAAGGTAATTTCATTGTTATCTCCTTAGAAAAATGAATTAATTATTTAATATTTTATATTAAATGTTGTTTTTTATTAGTTTATTTGCTAATTATTACTGAAGCAGGTTTTAAAATTCTGTTAAATAATGAATAACCATTGCTCTTTACTTCAAGAATTGTGCCAGAAGGTTCTTTTGATTCAATGCTTTCAGTAATTTGTTGACATTCGGGGTCGAATAAATCTCCTACTTTTGGTTCAATTATTTTTATTCCGTTATCTAACATTAAATTTTCAAAATCATTCTTAAGCATATCAAAACCTTTTGCAAAATTATTAACAGCGATATTATCAGATTTTTTTGCAAAATTTAAAGCATTGTCATATTTTGTATATATCTTAATGAACTCTTCTAAAAAGTCTTGTAGAGCATATTTTTTTAATTCTGTTTTTTGTTGCAATAAATGCTCATCATTTTTTTGGCGATGTTCGTTTATTTTTGATTGTGCTTTTGCTTCCAATTCGATTACTTGATTTTTAAACAAAATTTCTTGTTGTGTCTTTTCTTGTTTTATTTTTTCATTAAGTTCGCCTAATTCCAAAAAATTTAGTTGTAATTTTTTAATATTTTCATTCAATTCTTGATTTTTAGCAAAAACTTCTAAATACTTAGGACTTGTTTTTTGAAAATCAACAATTATTACCTTAAAAATTTCATTATCGATTTTTAAAACTAACTCACCTTTTTCGTTTTGCTTTCCTTCCAAGATGGTTTTTTCAATATTTTTATCTAAATCAAATCCAACAAATCCCTTATATTCAATTTTTTCATCTTTGCTAATTTTTCTAATGCACTCTATATAATCGCATTTTGAAATATTTTGACTCATTTATTCTCCTTTTTCTTCTTCTTTTTTAATAATACCTTCAAATAAATTTATCGCTTCTAATGATTCTTCAACATCTAAATTTTTAGGGCCTAATATACTTATTTCTTTAATATTTTTGTGATTAATAAATTGAATTCTTTTTGAAATAATATTTAAATTAGGATGTGAAAAATCTAGTTTTATGTTTTTTTCTTCATCAAGATTGTTTTCAATCGCTTCTCAAACTGAGTGGTTTTCAATTAATTCTAATGTCTTAGCGATTTCTTCTCTGCTTATTTGTTCTGATAATATTATTACATTTTTATTAAACGATTTATTCTCCAATTTTTCTTCAAAACAAAAAATATTTTTGATAAATTTCTGCATTATTAATTCAAAATGTTTAACTTGTGTAGATAATATTGGGGCTAATGATTCGGTTTTTTTAGCAAGATTAATTAATGGAGTATCAATCAATCTTTCTTTAAATAATCTAATTGCAATCCTTAAATCATTTAATTCAATCCCACTTTCAAACTTAAACATTTTGTTTTGAACATTTCCTGAACTTGTAACAATAACAACAACTGCAGATTCTTCATCAAGCGGTGTCAACTGAATACTTTTCAATAATTCAGATTCATTGTTTGAAGTTGCAACTACGGCAAAGCCTGCCATTTGGCTAACGACATTTGCTGCTTCTTCTAATGTTGTGTTGATGCTTAACCTACGCTTAGATAAAAGATCTTCAAGTTTTTCATTGAAATATTTTTTAGCATTATAAAGTAAATTTTTTGTATAGTATTCAAGACCTTTTGAAGAAGGAATTCTTCCTCCTGAAACATGTTTTTTTTCTAAAAGACCTAGTTTTTCTAATTCTGCCATTGCGTTTCTTATAGTTGCAGAGCTACAAGATAATTTAAATTTTTCAACTAATCTTTTTGATCCTACTGGTTCGCCGGTTTTAATGTACATTTCAACAATGTATCTAAAATATTTGATTAGTTTCTTATCATAATTATTGCATATAATTAAAGACTTATTTGTGTTTGCATTAGAAGAATTTTGCTCCATGATTGCCTCTTTCAAAAAAATTTTAGCACAATTTAGCACTTTAATGCGTTAATTGCTAATTTTTTAAAGATTAATAGTTTAAAAAAACTAGGAATTAACCTAGTTTTTAATTCAGTTTAACTGATATGGCGCGCCCGGCAGGAGTCGAACCCGCAACCTTTTGGGCCGTAACCAAACACTCTGTCCAATTGAGCTACGGGCGCATTGGAGCCACCAACCGGATTCGAACCGGTAATCAAGGTGTTGCAGACCTATGCCTTGGCCGTTTGGCTATGGTGGCAACACTGGCATGCATGTTTGCATATAGAATTATAATACATTTTATAAATTTTTAAAATAAAATTAGTAAAGAAAAAAGCGCAAGAGGGGACGCTTTAATACTATATATTTTCTTTAAATATGCAATAATTATATTATTTTTTTTAAAAAAATCATTTTTTATTATTTTTAATGCTTTATTTTAAACAAAAATTAACAATTTTAGAACGAAATGAACTTTTTCATCAATCTTAAGACTGTCAATTTAAGCAATGATTCAATCCTAGTTATACCATCATTACCAGGCATTTTATTAAATATTATATGTTGTTTTGAAGAAGTAAAATCAATAGGCGAGCTCATAAAAGTAATTTTATTTTTTTGTTCACGTTCGCTCAATATATTAAATATATTTGTAATATATCATTCCGGTTTTACTCCGATCGCAAAGCGATCTATGAATAAATAATCAACAGAAATCAAATTTTGAACAATTGAATTAATTTCTGATGAGTTTTTTAAATTATTAGACATGACTTCAAACAAATTATTGTTGTCAACAAATGCTACACTTTTGTTTTTCTTAGTGCCAAATAAGAAAGCTAAAAATTTTAAAATATTTCTTGCATTAACGAGATTTTGATCAATGAGCAATAATCCTTGATTTGATTTCATTGAAGTAATATCTTTGATTTCATTTTTTAGTTCTGGCGCAAATTTTTCCAATGATTGGTTTGTATTCAATACTATATATTGAGGTTTCTTTGCTTTTGCGTTATTAAAATAATAAATTCATTCATTATCCATGCTTGTTATATTTGTGAGTCAAAAGTTATTCATTTCTTTTTGTTTTCTAAAAAAACCATCGTTGCTTAAATCAATTTGCAAAATTCCATTATCATCAACAAAGAGGGTTCATGTAGGGAGTTGTTTATGTATTTCAATATAGCTATAATATTTTTGCAAATAATTCATTCCTTTTTGGATTTGTTTAGAATTTAAATTCAGTTTTTCAATAATTGCTTTAACATTTGGATCGGCTAAAATTTTTGCTTTTTCTGCCTCTAAATCAATATTTTTCAAAAGAGAATTTTTAAATAATTCATCGAATATTTTGTTTTCCATTTTTTTGCTTTCCTATTTTTTAGTTAGTTCAATCATGTTCATTTGCAAGATCTAAAAAACAATTTAAATCTTCTCTAGTAACCGATTCATCTTCATTTTTTTTGATATTTTTTAACTCTTTATTTGCATTTTGTTTTATTAAATAAACATTTTCTAATTCTTGATCAATTCTTTCGGCATCATATAAATTTCTATGACTAAAATCTTCTGCTATTTTTTCAATATATTTGCATACAACTGAATGATTTATTTTAATTGAATAATAAATGAAAGCATTTATTGAAAAATTATTAAATTTCATTCTTTTTAATTTTCTAATCATTTCATTTTGGCTAAAGCTTAAATTTGTTTTTGTATATTGACTAATAAATTGTTCGGGTTCTATCTTATCCTTTAATTCATCTAGAGTAATTGAATTAAAATTCAATTCTTCATTTTTTGTATTTAAATCAATTATATTTTCTAGATTAAATACTTCATAAAATTTTTTAGAAACATCATTAATTGTTGTTTTATCAAATTGATAATTTGCATTTAATTTTAATAATTCTTCATATTTTGATTTAGTGATTAATTTAATTAACATATTAGAAATAATTTTATTATTGCTTATTTCATTTGCATTCAATGGAGCATTTAAACTAAAAATATAAACACCATCCTTGTTTAAATAACTTTTTAGTAAGCTTAGCGCTTCTAGTTTACTACGACATTCTTCAAATTCTTGTTCATTTAATAATAAAATATCTTTAATTTCAGAAAAATTAAAAAGTTTATTTTTTGTATAAATATTTTTTAAATTATATAAAAATTGATATAGAAATATGCTACTATGGCCTAAAATCGGAGCATAAAAAATTACTAGATTTTTAAAATCGTTTTGTGTAATTTCCCTCTCGCTTTCAATAAAAAAATTTTCAACCATATTTAGCCCCTTTCTAATTAGTCGTGTACAAATATGATAGATTGCTTAGGAAATAAAAATTAATTTTTCTTAGGTTTTTTAACAATTTAACTACTTATTAAAAAGTTATTAACAATGCACCGCATGCCTTTTAGCCTTAAAACTAGATACTTTTTAAACTATTTTTATTTTCTAACAGTAAGAAGGAATTTTTTAATGATTTTTTGGTTAAAAATGTAATAAAAAATAGAAAAAATTTTTCTTGCAAAATAACTTTCACTATGTTTTCTTATAGTTTCGAAAAATGTTTTATTTAATAATATAATTGAAATATGAAATTAATTAACCTTCACTTAAATACTATATTTTCTTTTTTAGAAAGTACAATAACCATTAATGATTTTATTCAAGAATTACTAAATAATAATATAGAGTATTTTGGTATTACAGAACATTGCAATTTTTATAGCATGCCACATTTTTTAAATGAAGCTAGAAAACATGATTTAAAGCCTATTTTTGGTCTTGACGTAAATGTAAAAATAGAAAATAATTTGTATAGATTTATAGTTTACTGCCAAAATAAAGAATCTTTTATGAACCTAAAAATGCTTTCTTTAAACTATGGAAAAAAAGGCTATATAGATTTAGCTGAAATTAGAGATTATTCTAATTTAATATGGATAGAACATCCAATTTTTGGCTATTATAAAAAAACTAATAATATTTTAGAAATTCAAAACCATTATTTTGCAATAAGCCAATATGATATAGACAATAGTGAAATACAAAATATTTTTGATAAGTGTTTATTAATTAATCACAATTCAATATTAAAATATGATGATAATTCAATAATTAAAACAATGTCAAATATTTCAAATAATAAAATATTTGAAAGCATTTACGAAGAATTTAAAATTGATATAGAAACAAAAAACAATTCAAAATTAGAAGAATTAATTGAAAGAACAAATAATTTTGCTAAAAAACTTTATTTTGAAATTGAAACTAAAGGTTTCAAAATGCCCAAATTCAAAAACGACAAAAATATTGCTTCTAATGAATATTTAAAATTGTTATTATTTAAATCAATAAGAGAAAAATTTGATAAATATTCATGAACAGATGAATACCAACAAAGGTTTTTAAAAGAACTAAAAGTTATTGAAGATCTTAATTTTGCTGATTACTTTTTAGTTGTTCAAGATTGAGTTAATTGAGCAAAAAATAACAATATTAAAATAGGACCAGGAAGAGGTTCAGCAGCCGGTTCATTAATAGGCTATCTATTAAATATAACCGAAGTAGACCCTATTAAATATGATTTGATATTTGAAAGGTTCCTAAATTCTAATAGAGTTACTATGCCTGACATTGATATTGATGTTCAAGATAATAAAAGAAATGATGTTATTGAATATTTATTAAAAAAATATGGGCATGAATTTGTGGCAAATATTGTTACTTTTTCTTCTCTAGGTAAAAAATCAGCGATTAGAGATATTTTAAGAATAAACAATATTGCCCCAACATTAATTAATGAAATATCAAAAAATATTGAAAATGATGAACAAGACCTATTAGTTGAATACAATGAAAATAGAAATTTACAATTATCATTAGCAAAATTAAATCCTAATGATGAATCATTTAGCACAAGAATATTAAACCAAGCAGCAAGGATATCAGGATTTTATAGACAAATTGGCACGCATGCTGCCGGATTAGTAATTAGCGACCAACCCGTCATCTCAATAGCACCAGTTCAATATAATTCTGAAAATTTTCTTCAAACTCAAATAAGTATGGAATATTTAGAAGATTTCGGGTTAGTTAAAATGGACATACTTGGTTTGAAAACATTGACGACTATTTCAAATATAGTTGATCTAATTTATCAAAATCAAAGTCTTAAAATTAACTTAAAAGATATAGACTTAACCGATCAAAAAACATTAGAATTATTGAGCTCTGGCCACACAAGAGGCGTTTTCCAAGTTGAAAGTCCAATCATGATAAAAGCCTTAAGACAGGTAAAGGTAAATAATTTTGAAGATATTGTTGCAATAATTTCATTAAATAGACCAGGTCCAATGAAAAACGTTTCGGTTTATGCAAAAAGAAAATTTGGACTAGAGTCGATACCAAAAATAAATAATGAATATGATAAAATTCTTGATTCTACTTATGGAATCATTGTATATCAAGAACAAATTATGAAAATTGCTCAAGTTGTTGCTAAAATGACATTCATTGAAGCTGACAATCTAAGAAGAATTATCTCTAAGAAAAAAATAAATGAAATGGAAGATATTAAATCTATTTTTATTGAAAGAGCAATGAAGAATGGCTATGAAAAATCTTTGGCTACAAATATCTTTAACAGCATAGAAAAATTTGCATCATATGGATTCAATAGATCTCATGCGGTTTCATATTCAATTATAACTTTTCAAATGGCCTTTTTAAAAGCTCATTACCCCTTAGAATTTTATACGTCATGTATAAGCTCGGCACACGGCTCTCAAGATACAATTGCAAAGTTCGTTAACGAGGCAAGTGAGTGAGGGGTAGAAATAGTTTCTCCTGACATTAACCTTTCTGAATCAGAAGCTATAATTAAAAATAGAAAAATTATATTGCCACTTACACTAATTAAGGGTCTTGGTCCCGAAATTGCTAATTCAATTGTTGAAAACAGATCAAAATATGGCCCATATAAAAATTTCCTAGATTTCTTATTAAAAATTTCTAGCTCTCAATCAATTGGCAAAAGTACAATTGAATTATTAATAAAGGCAAACTCTCTTAGATCGTTTAATTATAATCAAAACACCTTGTTGAATGAGATTCAAAAAAACAATACTGATACAATGTTATTTATAAAAATGTTTAAGAATAACATTGAAGGTGTTAACCCTAATATAATAAACTCTTATAAACCAAGTGAAATTATTAATCAAGATATCATGTTTGAAAAGAAAAACGAAATTGAATTATTAGGTCAAGTTTACAATTTTAATTCAAGCGCAAGCAATGGAATGCCTGGACAAACTAGTTTTATAGACATGCACGTTGGAAATGAATATTTAGCAATTGTTAACTGTACAAACGTAAAGCAAAAAATTTCTAAAAAAGGTAATAACTATTTTGAATTAGATTTAGAAGCAAACATGCAAAAAATAAGAGCATTTTATTTTACATCAAATCAAGAAATACTAAATGTTAAGGGAAAAACTATCAAAGTAACTCTAGAAAAAAGAAATGAAAATATTTTTTATTTGCGTTCAATAAAGGAAGTTTTAAATGAAGAAAGATAATGAACAACTGTTGATTATAGATGGTACATTTTTAGCATACAGATCCTATTATGCAACATCTTATAACGCAACTCAAATTTTAACCACATCAGAAGGCTTTGAAACCAATGCGATAGTAGCATTCTTTAAAACACTTTTTTTATTACTAAAAAAATTAAATCCTAAATATATCTATATAGCCTTTGATTCGCATGTTAAGACGTTTAGGCATGAATTATTTCCTTCTTATAAAGATGGTAGAAAAAAAGCGCCAGAAAGTTTTTATCTACAGCTTGATTTAATTCAAGAAATATTAACAAATATTAATATTCAAAACCAATATTATAATGGCTTTGAAGCTGATGATATAATTGCAAAAGTTAAAAATAAATTTAATTTGAATACAATCATTTTTTCAGGAGATCAAGATTTAAATCAATTAATAGATGAACAAACAGCAATCATTAAAAAAGATAAAACTGGTTCTTTCTTTTTTCTTAATTCAAAGAATTTTTCAGAATATTATGATTTTTTACCAAATCAAGTTATTGATTATAAAGCATTAGTCGGTGATAACAGTGATAATTTTAAGGGTGTTGTAGGAATTGGTCCTAAATCTGCTAAAACTTTATTAGAAGATTATAAGACTATTGAAAATATATACAATAATATTGAAAAAATAAAACCTTCAATTGCCAAAAAATTAATGGAATATAAAGAAAATACATTTCTCGATAAATATTTAGCAACCTTAAGGATAGACTTTGATTTGCAACTTCCAGAATTAGAAAATTTAGCAATATCTAATATTGCAATTTCAAACAGTGCAAAACAAATTATTGACAAATTTGAACTATATTCTTTGGAAAATTCTTTGAAGAATTTAATCAATAAAAAATAACAAAAGTAGGGTTTTTCCTACTTTTGTTATTTGCCAAAATGAAAATTCAAGTGCAACACAAATTTGAAATAAAATTTAGTTGTGGGGCACTTGAATTTTTTTAAAATAAAAAGCTCCACAAACGTACCAAGGGAGCCTTTATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGTTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCGAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAGTTTTCCAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGAATAAACACAAATAAATGAAAAATAAGAAAATCCGATAAATTAAGGCAATATTATAAAAAAGGAGGCAAAAGAACTGCTTCGGTTCTTAGTCGTCTAATAAAAAGTGCAAATTATGTGTTTCCAATATGAACAAGACCAAAAAATATTGATTTAAGGTTGGAATACGGGCATTGAGAGGCAGATTTGGTTCTTGGAAAAAGAGCAAATGGCTATAAAAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGCGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGAAATGGCATGATAAGAAGAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGAAAATCTGCATATGAATTGTTTATGTATGAAAATTTTGGCGAAATAATAAACTAGAAAAATTTAAAAAATATAACATGTTGTTTATCACAACGTTTTTTCTATGCAAAAATTTTAAAAATCGCATAGAAATTTTAATAACTTAATATAATAAAAACATCAAGCACAAAAGTGTTGCACTTGATGTTTCATTCTGGGAAATAACAAAAGTAGGGTTTTTCCTACTTTTGTTATTTTTGAAGTTCTTGCAATGTTTTTGCTACTCCAGATTTTTTATATGAATATCCTACATAATCTGCTTTTTGTTTTATGTTCTTTGTAGAATTTTTCATCGCTACAAAGCCACCTAAATATTTTTGAGTAACAACATCATTGCCCGAATCGCCTAAGTGAACTGCTTCTTTATAATCAATATTTAACAAATCACATACAAATCGATCGCCAATTCCTTTATTAGCATTAATATCGCTAATTTCAATTGAATATCCTTTTGATACAATATGTGTTGAAATATCATGAAAAGCATTTAATAATTCATCTCTAAGTTTTATTATTCCTTTTTTTGACTTCCCAAAAGTTAAAATTTTTGTTGTTGCTGGCAATGATTGAATTTCATCATATGTAACTTTGTTTAGCTTCTTTAATCAAGCTCTAGCGATTCTAAATTTTGTTTTTGTTCCATATATTGTATCGCCACTATTAAAAATAAAAAACATTTTTTGTGCAATTAAAATTTCAATTATTGATTTCAATGTATCAGGGTTAATTTCATGGCGTTTCAAAACATTATTATTTTTGTCTACAATTACTCCACCATTTTGGCAAACAGCAAACGGTGCATTTATTTTATTAGTTAAATCCAAAACAAACTTTGAATTTCCTCTACCTGTTGACAAAATAACAGGGATTCCTCTATTATTGAAGTCCTTAGCAACCTCAACATTTTTTTCGCTTATCATAATTTGTTTTTTGCGAGGCAAATCTAGATATGTGCCATCCATATCCAAAAAATATGCCTTAGGTTTAAAATCAATCATAATTTTCCTACTTATTTAAATTGTTTAAAATTTCTTCAACTGGCAAGTATCCTACACTTTGAGAAACTAACTTACCGTCTCTATAATAAAATAACGATGGAATATGAGATACATTCATTTCTCTTGCTAAATCAATGAATTTATCTACGTTCAAATCAAAAACATTAACATCTGAATTTTCTTCTGCTAATTTTTCTAATTCTGGGCCAATCATTTGGCATGGACCACATCAAACTGCTCTAAATGCAACAATTGATTTTCCTTCTAAATATTCATTCTTTTTGAATTCTTCAACTGATTCAATAGTTTTATACATAATATTTTCTCCTTTAATATGCTCTTGCAAATAATACAAATCTTTTTGTTTTTCTATTAGTTCAAAAACAAGTATCATTTGAAACTTTTATTGGTTCTTTAAATGGAATACACCTTGTTGATGCTCCTGTTTCTTCTTTAATTTTTACTTCATCATCACCGTTACCATCAAATGCAGCATACACTCAATGACCCGCAGCAATTGCTTCCTTAAATTCTTGATAATTATTAACAGTTACAATATTATTTTGTAAGCGTTGTTTAGCACTTTCAAATAAATTGTCTTGTATTTTTTGTAATAAGAGACTGGCAATATCTGCTGCTTCAGCAATTGTTGAAGTAATTTTTTCTAGGTTATCACGTCTAACGAATAATACACTATTATTTTCAATATCCTTTGGACCAATTTCTATTCTCAATGGTGTACCATGAATTTCTGAATTTGCTGCTTTAAAACCTGGACCTTTGTTTGAAGAATCTATGTTTGCAACAATATTTTTTGCTAATAATGCATCTTTTAATTCAAGGGCAAGCTTGTGAACTCGTTCATCCTTTGAATTAAAAATTTCTAATATATCAACTTGAGTCCTTGCTATTTTTGGTGGAATGATTATTCCTCGATCATCACCATGAGTCATAATTAGCGCACCAATTAATCTTGTTGTTGTCCCTCAACTGGTACCAAAAGGATTAATTAATTTATTTTCATTATTTTTGAATGTTATATCGAATGCCTTAGTAAAATTTTGTCCCAAATAGTGACTAGTACCCGATTGAAGTGCCTTTCCGTCCTTCATCATTGATTCTAAGGTGTATGTTATATCTGCGCCAGCAAATTTTTCATGATCCGTTTTGCAGCCTTCTACTACTGGAATTGCTAAATTTTCTTCAATAACATCTTTATAAACCTTGTGCATAGTCTTGGCAAATTCAATGGCCTCTTCACTACTTGAATGGATAGTATGACCTTCTTGTCACAAAAATTCCCTTGTTCTTAAAAATGGATTTGTTGTTTTTTCTCATCTTAAAACATTAACTCATTGATTATAAATTATTGGCAAATCATTGTAAGACGAAACCTCATTTTTGAATAAATCAGCAAACAATGTTTCTGAAGTTGGTCTTATAAAAAACTTTTCTGCCAATTTTTTGCCGCCTACTTCAGTAACAGTTGCAAGTTCAGGATTAAATCCTTCGACATGATTTTTTTCTTTTTGTAATAAGCTTTCTGGTATTAAAAGAGGTAAATAAACGTTTTGAACGCCTCTCTTTTTAAATTCAATATCCATTTTTGCTTGAATATTTTCTCAAATAGAATATGCCAATGGTTTAAAAATAATGCATCCTTTTGTTGCACCATATGCCATTAAATCCCCATTTTGGATAACGTCAGTATATCAACGCGCAAAATCTTCATTTTGTGGTGTTATTTTATCTAATTTTTTCATGTAATTAATTATAAATAAAAAAGATAAAATTAAAAGCACAATGTGCTTTAATTTATTTTTTAGATTAATTTATCAAAAATATCTTCACCTGTTTGAACGGTTGGAACATTGAAATTTCTTGCAACAATATTTCCTGCAGTTCCCAAGCCTTTCAAAGGTTCTTTAAATACACATGGTGATTTGAATTTCTTTGAAAATATTGTCAATGGCAATGCTTCTCTTGTATGATCAGGGCCTTTTGTTGGGTCATTTCCATGGTCAGAAGTTATAATTAACAAGTCATCTGATTTCATTGCGTTTATCAATTTTGTTAATTTAATATCTAGCCTTGAAATATTTTGTGAATATCCTATTGGGTCTTCTCTATGTCCAAAATGGCTGTCAAATTGAACAAGGTTTACAAATACAAATTGGTTTTTGGTATTTGAAGAAGCAATGTCAATTGCAACGTCCATATTTTCATCATCAGAAGCTGGTCCATAAACTTTGTCAATACCAACATGACTGAAAATGTCGTTAATTTTACCAACAGCAACTACTTCAACACCTGCTTTTTGTAAATCTTCTAGAATAATATGTCCTGTTGGTTTATTAGCAAAATCATGACGATTGAATGTACGTGTGAACTTTCCATTTTCATAAACAAATGGACGTGAGATAACTCTTGCTACATTTCATTCAGGTCTTGATGAACAAATTGCTCTTGCTCTCTTTGCATATTCAGTTAGTTTTTCAACACCCAATGTTTTTTCATCCCCAATAATTTGCAATGTCGAATCAGGAGAAGTGTAAATTATCATATCTCCTTTTTCAATGTGTTGCAAACCTAATTCGGCCAATATAACTGTGCCTGATGCATTTCTATTTCCAATAATTTTTCTATTATCAAACGCTTTTGATAATTCTGCGACTAAATCAGCAGGGAAACCTTTTTCAATGAATTGTGGGTTTGGAACTTTTGTATAAATTCCCATCATTTCTCAATGTCCTGTCAATGTATCTTTTCCATTTGACATTTCATAAATTTTTCCCATATATGCCAATGGTTTTTTATTTCTTGGTACATGTTTTGAAGTTTCTGCTATTTCAGTAATTCCTAATTTTGTTCATGTAGGAATTTCTAGTGGCAATGCTTCTGATGCATGCAACATGCTATTTGCACCTTCATCATGAAATTCAGCTTGGCGTGGTTCATATCCAATGCCAAGACCATCTGTAACTATAAAAAATATTCTATTAAATTTTTGTTCTTGCATAATTAGTACTCCTTTTTAGCAATCACAATAATAATTGGAATCTCATACTTATTTTTTAAATTGATTTTAATTTTAAATAAAAATAATTAATGTTTTAATAAGTTAGATAAGTAATTTTACTATTAATTTACTCACTTATTATTTTATTACAATAATGAATTTAAATTTATTAACTTTCAATGCATTAATAGACTTTATATTGTCCGGCGGGCATTCTATTGATTTTTTTAGCCTCCTGTGTGATAAAATTAATATGTTAGATTTATATATTTATTTAACAAAAGAGGTAATATGAAATTTAATGAACTAAGAACTAAAATTGTTGCAACAATTGGCCCTTCTTCAGATAATTATGACACAATGAAGAGTTTAATTGAAGCCGGAGTTACTACAATTAGAGCCAATTTTTCACATGGCGGTCCTGAAACTCATGTTAATAAATTTGAAATAGCAAGACAAATTTCAAAAGAACTAAACATTCCAATCTCATTAATGCTTGACACAAAAGGCCCTGAAATTAGAGTTGGAAAAATGGAAAATGATGGAGTTACAATCCCTGCAAATCACATTTTAAAAATTAGAACAGATGAAAATTCATACAAGAATTTTTTAGGCACCCAAGATCAAATTACTGTTTCATATAGAATGGACAACGATCTTAAAATAGGGGATAAAGTTTTAATTGATGATGGAAAATTAGTTACAACTGTAAAGGATATTAAAAAATTTGAAATTACTGTTGAAACAGTTAATGAACATTTTTTAAAAACTAATAAAAGAATTAACATTCCTGGTGTTAACTTCTCACTTCCTTTTTTAAGTCAAAAGGATATTGACGATATTATATGGGGTGCAAAATACAAAGTTGATTATATTGCAGCATCATTTGTAAATTCTGCTAAAAATGTAAAAGAAATTAGAGACATATTAAATGCAAATGGCGGAGAAAACATTCAAATCATTTCAAAAATTGAATCTAAGATGGCAATTAATAACATTGATGAAATAATTGAAGCTTCAGACGGAATTATGGTAGCAAGAGGGGACCTTGGACTTGAAATTCCTTACTATGATGTTCCTTACTACGAAAAGAAAATAATTCGTAAATGTAGATTAGTCGGCAAGGAAGTTATAGTTGCAACTCAAATGTTAGATTCAATGGAAAACAATCCTATTCCAACAAGAGCGGAAGTTACCGATGTTTATTATGCAGTTGAATTAGGTGCAGACTCAACAATGTTATCCGGAGAATCTGCAAGTGGTAAATACCCTGTTGAAGCAGTTAAAGTTATGTCTAAAATTGCAAAAAGAGCAGAAAAAGAATATTACAATGAATTATTCTATGGCAAACAAATAGAAAAAGTAGCAAATCATCCTACTTCAAGAATGCTAATTGCTTATGAAGTTGCCAAAATCCTTCAAAGAGGTGAATACAAATTTGCCATTATATTAAGTCACACCGGAAAATTATTAAAAGAAGTTGCTAAATACAGACCAAACGCATTTATTGTTGGAGTTGTTAGCGACAATAGATTAGTTTGAAGCTTTGGAATTACTGCTGGTGTATTCTGTGAAAAACATTCAGAAGCAGTAAGAGCATTAATTAAAAACAATCACAATGCTGCTTATAAAATTTTAGGCGAATATGGCGCTAAAAAAGGCGACAAATATATTGTGGTGGATAGTTTAAATATCACAACCCATGAATACTAATAAAAATTATTAATTTCAAAACGAAAAATTAGGCTTTAACTAGGCCTAATTTTTTTGTCTCGGCTTAACCGGACTTACTTTTTTTATATCGCTTATTTCTTATTTTTAAAATAAAAACATGCTTTTTATTTGTTTTGACTACCTTCTAAAAGTCGGTCGTAATGAGATAATTGCTTATTTTTTTGTGACCATATTTATAAATTTAAATTAATTAATTCAGAGAATTTTTTTATATAAATATTTTTCCCAACATTATTTTTTTCAAGCATTGATTTATATGTAGATAAAAATACGGATTCTAATTCTTTTTTTTGTTCTTCGTTAAATTTTAAAAAATTTACAAAATATAAATTAATTTCCTTTATATTTTGATTTGCGTTAACTAGGTTTTGTGCATGTTTTGATATTGAATAATTTTCACCATCAACAACTTCCTTCTCTGTTTTTAGTATAGTCGTTTCAACTATATACTGAATGTTGTTTTTTGAATCATAATAATCGAAATCTGCCGAATGTCCGGGAGCAGAAAAAATTGGATACAAATTCTTTGTTAATTTTGTATGACATATACTTTTAAAATTTTGCGGATTAATATTAAGGGAATATGTGAAGAATAAATTAACAAAATATTCTGCTAACGCAAAATTTGGCACGTGTTTTAGGGCAATGTATTTTTCTGATGGTGCTTCTTTAAATTCAAATTTTTCACTTTGAATTTGATTTAAAATATCTATTACTTCTTCATATTTAAATGGAAACTTGATATTTTTTTCAATATTATCCACCTTAAAAATTGGCAATGAATAAAAATTGTCTTCATTTAAATATTTCAAATTTTCTAAATTAAAAAAATTATTCAATTCAGATTTTAAAATCGTTTTTGAATTGCTATTATATAAACCCAATTTACACATTCATCTATTGAACATGTCCATATATTCTTTTTCGATATTAAATTCAATAGAGATTTTTTTTAAATCTAGCAAAAAATCTTCTTGTAAATGATTAAATATGTAAAAATTTATTTTATCTAAATAGTTTACATTTCTTTCTTTTGAGGGAATATTTAATAATCTTTTTATATATTTAAATTCTTTGGGTTTCAATTCTAAATAATCACTATATGACAAAGGTTCTTTGATTCTTAACAAATTAAAAATTTTTTCTAAAATCTTATATTCATTAATTTTCTTTTTAAATAAAGACTTAATATTAGTTATATTAGAAACATCTGCAATCTCTTCTAAAATTTGCTGTTTATTTTTTGTATATAGATCCTCAAATTTATCTTCTTTATAATCAAAAACCGCAAATGCTCAATTTAATTTTTCAATGTTAATCGATGATTCATTATAAGAAATTATAAAATTTACCATTTCTCTGAATTCTTCTCAATATAAGTACATCTTTTTGAAAAATAATTCCTCAACCGGGCTGTCTGTGTCAAAAAATTTTTTTGGTTCAAAATTAAAAACTAAATTTTTAGAATTTTTTAGAAATCCTCATTTTTTTAAATGACTTATCACGAAATTAAATCTACTTTTTATTTTTTTATCATTGCTATAATTATAGATTTTTTGATTTTTAACATATTTTTTAAACTCTTCGTCTCAATTTACATTGAAATTTTTTTGAAGATTGTTTGACAAATTATTTTCAATAGATATTCTCTCATAATTTACTCAAGATAAAATAGAAAAATATTCATTTATAGGTTCAGCAACTCTAAATTCAGTACCACCAAATTTATTAAATCCCTGCTTTTTCATAATTAGTTACATAAACTTCGTCATCTAGTTGATCTCTATTTTTTCTTTGATAATTTGAATTTGTGTATTGCTTATTTAAATGAAAAACTTTATATTTTTTTGACCATTCAATTAATTCAGTATTTGATTTGCCCTTCGATTGAAATAAATTTGATAATCCTCATTTTATGTTTTTTGAATCTAACAAATCTAATAATTTAAATAATTTTCTGTCTTCTTCTAATGTTCAGGCCTGATTATATATTGTACCCGAAATTAAGTATGGAGGATCAAAATACCAAAAATCGTCTTCTCTTGTTTCTTGCATTTTCAAATATATAAATTCTTGAAAATCCAATGAAGAAAAATCAATATTTTTTCTATTTAAAGTTGAAGAAAAATCGACAAGGCATTGTTTCCTTGTTTCATTTAAAATTTGTTTTCCGGAAGGAATATTAAATTCTCCTTTTGAATTAAATCTTATTTGACTATTAAATCCATAAATTATTAGAACTAATAAAACTATACAAGATTCATATGAATACTTATTTTTTGATAAATTATATTCACTTTTTATCGAATTATAAATTGATGAATTTACTCTTTTCGTTACGGGATCTAATTTATCAATTGAGCCATACTTCTTATATATATATATATATATATATCATTTAAAATTTTTATAGGGTTAGTATTTGAGAGAAGTTTTACAATATTGCACACTTCTTTTTGCTTATCATTGTAAAAATATTTTTGAGCTATAACATTTGCAATAACCGTTCCTCCTCCGGCAAATGCATCATAAAAATTTCTAATATTATTTGGAAATAATTTTTTTAATTGTGGTAATAATTTATATTTATCGCCCAAATAATACAATGGTGATCTATTAATTTTATTCATTTCTAGCTCATACCTTTCTATTCTTTGCTTTGCAGCACAAAAATATTTTTTATCTATCTCACATCCAACAAAATTCCTTTGCAATTTTAAGGCCGCAACGCAAGTCGAACCAGAACCCATAAATGGATCAAAAATTCAATCATTCTTATTTGTATGAATATTTATAATTTTTTCCATTAACTCTACCGGTTTTTGAGTCGGATGCAATTTATCAATTGATTTAGAAACGATTGAATTTTTAAATTCCGGTCTTAAATATTTTTTGCTTTTTTGTTTATTAAATGTTCATTTTTTGTTTCCTTTTACTGCTCAAATAGCAAATTCAAAATCTGTAACATATCTTCTTTCTACATTTCTAGGCATAGGGTTATTTTTTACTCATCTTAATAAATCTTTTATAAAAAATCCTTGATCTTCTAGTTCCTTGCTTATTTTGCCAAAATTTTTGTAATCATTAAATATTATTATTGAACCGCCATTTTTTATAAATGGGCTAATTTTCTTAATTCATTTAGTTTGGTCAAATTCATAATCTCATTTCCCAAAGTCTATTCCTGATCTGCCAATACTTTGAAAGTTATTTTTTTTAGATATATTATATGGTGGATCAGTTATAACAGCATCAAATAGTTCTATATTTAAATTTTCTTCTTTTAATTTCTTTATATATTCTAAGCAATCTTTATTCTTAATAATTCAAGTCATATCATCATCATAACAAAAAAAACGAAAATAACGACTATACAAAATTAAATTTTTTATAATTTTAGTTATAAAAATTTTCTATATAAAACTTTAAGTTCATATAAGAACAAATAGATATTCAACTCTAAATTTTCTTAATTCTCTTTTTTTATACACTTACTTTAATATTATAAAAAACACCAAGTGTTAAAATAATATACTTGGTGTTTCGCTTTAAATATTCTATTATTTAATAACTTCTTTGCCCATGTATGGAACTAAAACTTTTGGAATTGTGATTGAACCATCTGGGTTTTGATAATTTTCTAAAATAGCAGCAACAACTCTATCAATAGCTAAGCCTGAACCATTCATTGTGTATGCATATTTTGTGTGACCATTTTCATCGCGATATCTAATTTTTGCCCTACGACCTTGGAAATCTCTGCATATAGAAATTGAGCTTGTTTCTCTATATCTTTGTTCTGATGGAATTCATAGTTCCAAATCAATGGTTTTTGCTGAGCTAAATCCAATATCGCCAGTTGAAAGCATCATTTCACGGTATGGAATTTCAAGCAATTCAAGAATGCTTTTGGCATCATTTACCATTGATTCTCATTCTTTCATTGCATCTTTTTCACTGGTTATTTTTACAAGTTCGACTTTATGAAATTCGTGTTGTCTAATTAAACCTTTTGTATCTTTTCCACTGCTTCCTGCTTCTGAACGGAAACATTTTGTATATGCAACAAATTTCTTTGGCTTAGACAAATCTAAAATTTCGTCATAATAATAATTTGTAACTGGAACTTCTGCAGTTGGTATTAATCAAAGGTCATTGCCTTGCATTTTAAATAAATCTTCTGCAAATTTAGGCAATTGTCCCGTTCCATAAAGCATATTTGAATTAACTAAATGGGCGGGCATTAATTCTTTATATCCATTTGCAATGTGCGTATCAAGCATAAAAGAGGCTAAGGATCTTACTAATTTAGCACCATCATTTTTGTATAAAACAAATCTTGTGCTTGCTAACTTCACTGATCTAACAAAATCAACAATATCTAATTTTGTCGCTATTTCATAATGTGGCAATACGCCTTTTACTAACCCTCTTCCTAAATTTGGATGTTCTTTTAAAACTACGTTGTCATCTTCATCTCTTCCCACAGGAACTTCATCATATGGCAAGTTAGGAATTTGGTATAGCAATGTATTGACTTTCTTTACCAATGTGTCGGCTTCTTCATCAATTTTTTCGATTTGTTCTTTAACTTCAGCAACTTGTTGTAATAATTCTGAGCAATCTTGCTTGTTTCTTTTATTAATTCCAATTTGTTCACTTAATTTATTTCTTTTGGCTTGCAAAGTTTCTAAATTATGAATTAATTCGTTTCTTTTTTGAGCGTCTTCAAAAATTTCTTCGATAATAGCAATATTGAAATTACGTGTTGATAGTTTTTTAATAACTTCATTTTTGTTACTAATAATATATTTTAAATCTAACATTTTTAATCCTCTTTTATTTTTTTAAATGCTATTATATTTTACAATATTATTAAATAAAAGAATTACTTAATTTTTGTTAAATGTTATTTGACTTTTGCTTATTTTATATATAAAACAATAATTGATTATTATTGATAACCAAATTCTTATATTTGTTAAAATTTTAAATATGTTACAAAATAGCAATATTCAAGGTAAATTTAATAAAATAATGTGAACGTCTACTGATGGCCGAGTTGTTATAGTTTCATTTTTAGTATCTTCTAATGATGGTTTGAATCCTGTTAAAGTTAATAAATACAACAGTATTTCAGTAACTTTAAAAAACAATTTATTTTCTGAAAAAAACATTACTTTAAATAGCCACATTTATGAAATATCAGTCTCTAAAAATAGTTTTTCAAAATATCCAAATAGTTATATTGCAACAGACGTTATTGAAATAAACGAAGATGAAGAATATAAACTAAACTATGTTATTAAATTTTTAAAAAGCAGTCATTTCCCTGGTATAGGCGAAGAAAAAGCAAAATTAATTGTAAAAGATTTAGGGATTGACGCTTTAAAAAAAATGGTTGAGGATAATTCAATTGATTATTCGAAATATTCAATATCAGAATTGACATGAAACTATGCTGTTGAATTTATTAAAAATAATCCAACATTAATTGAAGATCAAATGTTCTTTTTAACAAATAATTTAACACCTTCTTTGTATGAAATAGTTGCTAAGAAATTTGAACATTTTAAAGATTTTATAGATGCTTACAAAGATAACTTTTACTTATTTTATTTAGACAATGAACGCATCTCGCTTGAAGATATGGACAAATTACATAATATCTTTAACAAATTTGAACATCCTTTTAAGCCTGCAACTCACTTGTACAAATCATTTTTAAAATATCTATATAATTCTGGAAACACTAGAATTTTAAAAGATTATTTATACGACCAACTAATAAACTTTGCAAGCCAAGACACTGAATGACCTAGGGATGTTGAAACATTCAATAATAGCGTTGAATTATTGGTTCAAAATAAATATTTAATCGAGATTGAATACGAAGATGGTACATACCTTAGTGCTAAAGATGTATTTGATATGGAAAAATATATTGTCAAAAGATTGAAACATATTGAATCTTCTTCAAACCCAAAATTCATCAATTTTATGCCAAGCGAAATGTTCCACCCTTTGCAACTTCAAGCAATAGAAACAGCACTAACTAGCAAATTGACATTGATTACAGGAAACCCTGGCACTGGAAAAACATTGATAACTAATGAAATAATTAAACAACTGCTTTCAGTCTATGATGAAGATGATTTGGCAATAGTTACACCAACCGGTAGAGCAACTATCAACATAAACAATATTCAAGAAAAGACGAATGCCGTTACCATACATAGTTTTTTAGAATGGGATGTTGATAATGACAAATTCATGATTAATGAAAAAAATGCAAAACCGATTGAATGTTTAATAATTGATGAATTTTCAATGGTAAGTGTTGATTTATTTTATTCACTACTTAAAGGAATAAAAAAGAATTCATTAAAACGAATCATATTAGTTGGCGACAAAGATCAATTGCCTGCCATTGGCGCTGGTTATTTAATTAGGGATTTTATAGAAAGCAATATTTTTCAAACAATTGTTTTAAGTAAAATTTTTAGACAAGCAGAAAATTTTGACATTATTCAAGATGCTCTAGATATTAATAAAGGCAAACTACCCGAATTTAAAGGAAATAATTCCCAATTTATTGAAACGAAACAGAGTGATCTAAAGTCAATGTTACTCTTTAAATTAAATGACTTATTGCAACAAGGCTATAGCAAGCGTGATATTGCAATTTTAAGTCCAATATATAATTACGAAACGGGAATTGATCAAATAAATGAAACATTAAATAATTTCTTTAGAAAATTAGATGGCAATGAACAAATAAAGTATCGAGACCATGTTTTGGCCATTGATGATAAGGTAATAAATTTAGTTAATGATCCAAAAACAAAAGTGTTTAATGGAGAAATCGGTTATATATCTCACTTTGGCTTTGAAAACAAAAAAAATTCATTTGAAAAGGTTTTAGCAAGTATCTCTGTAGATTTTGAAAACGATTCAAAAACTGTTTCTTATTCTAGAAAAGATTTTTTTGCTAATACATATCCTGCATATTGCACAAGCGTTCACAAATATCAAGGTAGTGAATGTAAATGCGTTATTGTAGTATTATTTTCAGAGGCTAAAAAATTATTATCTAAAAAACTTATTTATACCGCTATTACTAGAGCAAAAAAATATTGCGTTGTTATTGGAGAAAAAGAAGCTTTGATAACCGGTATAAATAATGATGATGATTCAAATAGAATAACCAATATTAAATATTTATGGAGGAATGAATAATGAAATTAATTGTTGGACTTGGAAATCCTGGTGTTGAATATGAAAAAACACGCCACAACACAGGGTTTATGGTAATTGACAAACTAGCAGAAAAATTGCATACTCCCTTAACTGAAAAAAAGTTTAATGGAATTTATTACATTGACAAAGAAGTTATTTTAGCCAAACCGTTAACCTTTATGAATAATAGCGGTGATTTTGTTAGAGCAATAAAGGATTATTACAATATTGTAGATGATGACATTATCATTGTATATGATGATTTAGATTTGCCAGTTGGAAAAGCTGTTATTAGACAAAAAGGTTCGTCTGGCGGACATAACGGAATGAAAGATATAATTGCTAAATTAAAAACTGATGAAATAAAAAGATTAAAAATAGGAATTGGTCGTGGTCAGAATGTAATTAATTATGTTCTTGGCAAATTTAGCTATCAAGATTTTTGCACAATTGAAAAAATAATTGACATTGCTTCCGATGCATTAATTTCTTTTATTAGTAATGATATTAGATTTGTTATGAATAAATTTAGTGGTGAACTATATCGTGAAAACAAATAATTTAGTTGAATTACAACATGATTTATTAAAAGAATTTAATAAATACAATATTGATACCAATAAAAAGTTTTTAATCGGAGTAAGTGGTGGCCCTGATTCAATGTGGCTACTTAATTTGCTTAAATCATTGAATATTGTTGTTGCGCATGTAAACTACAATAAAAGATATGATAGCAATTATGATGAATCATTGGTTTCACGATTTTGTGCTGATAACAATATCCCTTTAGAAATACTCTCATTACATAACAAATATTCTAGTGGTAACTTCCAAGCTATTGCTAGAAAAGAAAGATATGATTTTTACTTTGAAATTTCTAAAAAATATCAATGCGACTATATCCTCCTTGCTCACCAAAAAGATGATTTTTTAGAATCATCTATTATGCAAGAAAATTCAAACAGAAAACCCTTATTTTGAGGTATTAAGCAACTTAGCGTACTAAATAATAATATTATTTTTAGACCAATGTTAAATTTATGAACTAAACAAGAAATTATAGACTTTTGCAATGAATATTCAATATTATTTGCAACTGATTATACCAATGCTGAAACTCATTACACTAGAAATAAAATTAGATCGAACTTAAAGAAATTAGATCCAGAGCAAAAACAAAATAAATATCAATTTTTTGTCAATAAAAATATTGAAAATGAACATATTGAAAAACAAGTTTTAGAAAGTTTTGAAGATTGAAAAAATAATCAATTCAATGTTGATAAATTAGATAAGAATTTTGTTTATATTGACCAGTTATTGTTTTATACATTAAATCATTTTTATAACAATTTAAAACTTTCATTAAATAAGATACACGGAATTAAAGATTTTTTATTTTCACAAAATAGAACAAAAAATTTTAAATTAAACGATGAACAATATATTCATAAAATTAAAAATTTACTAATATTTAAGTAATTTTATTATAATTTACATATTAAATTATAAACAATAGAGAGGACAAATGAACAATCCAAAAGAAAAAAAACCTAGGAAAATTGGTTTGTTTGGAATAATTGGAATTTTATTTGCAATTATTGCAGTTATAACATTGGGATATATTATCAGTGATGTTGTTAAAGAAAGAATGTCTAACTTTCAAACCTTAAATTTTCTAGAAAATGTTATTAGAGGAACTGCTAACAAAAATGATGGAAACTATATTTCTTCATGAGTTGAAGACCCTTATAACGATACAATAAATATCACTATTAGAGGTACAAATAGTTTTTTAAGATCCTTAATTGGGAATAATTATTTTGTTGGCGATTCTTATAGTTTTATAGTAAAAAATGGAGGCTATATAGGTCGTTCAATATTTCAAGAATTGCCTGTATGAGCAGGTTTAAGTGGAAAGTCATATATAGAAGCATTGAATTCTTGTGCAACATTTAATGGTCAAGTTTTAGGAAATATTGTTGCAAGTTCAATGCCAAAGCCAAGCATTGCAAGCAAATATATAATTCCTGCTTTCTCTGCTTTTGTTGGCTTAGCATTTATTATTATCATTATGGCAATGTATTTTGGTAAGGGAAGAGGAATGCTAACTGGCGGTATTGATACAAACAAATCATTAGCGCAAAGAATATATTCAAATAAAAAATTCTCTGACATTGCCGGAAATGAAGAAGTAAAAGAAGAAGTAAAAGAATTAGTTGATTATCTTAAAAATCCTAAAAAATATGAAGCAGCTGGCGCTAGAATTCCAAAAGGAATATTACTAGGAGGCCCTCCAGGAACTGGTAAAACTTTAATTGCCAAAGCAACAGCGGGAGAAGCAAACGTTCCATTCTTCTTCATCTCGGCATCAAATTTTGTTGAAATGTTTGTTGGATTGGGCGCAAAAAGAGTTCGTGACATGTTTGCTGAAGCTCGCTCTCATGCTCCTGCAATAATTTTCATTGATGAATTAGATGCTGTTGGTAGATCTCGTGGAGCTGGAATTGGTGGAGGTAATGACGAAAGAGAACAAACTCTTAACCAATTACTAGTAGAGATGGATGGTATCAAAGAAAATAGCGGTATTTTAATAATGGCTGCTACAAATAGAACCGATGTTCTAGACCCCGCTTTATTAAGACCAGGACGTTTTGATAGAACTATTACCGTTGGCCTTCCTGATATAAAAGAAAGAGAAGCAATCTTAAAATTACATGCTAATGGAAAAAGAATTGCAAACAATATTAATTTTGCTATGGTAGCAAGAAGAACACCTGGTTTCTCTGGAGCACAGCTTGAAAACGTAATTAACGAAGCCTCTTTATTAAGTGTTAGAGAAAATACCGGAGTTATAACTATTGAACAAATAGATGAAGCTATTGACAGAGTTATTGCCGGCCCTGCTAAAAAATCAAGAACCATTTCTGAAAAAGAAAACATTGCAGTTGCCTACCATGAAGCGGGACACGCAGTTGTTGGAATAAAAATTAAAGGTGGAAATAAAGTTCAAAAAATAACCATCATTCCAAGAGGAAAAGCTGGCGGATATAATTTAATGATGCCTGATGAAGAAAAATACAATCGCTCGAAATCAGAATTACTAGCTATAATAACTTCGTTTATGGGAGGACGTGTAGCTGAAGCAATTATTTATGGAGCAGATAATGTTTCAACTGGAGCAAGTGATGATATTGCCAAAGCAACAAATATTGCTAGAAAAATGGTTACTGAATGAGGTTTATCAGACCTAGGACCAATTAAATATAAAGAAGATTCTTCAAATCCTTTCTTAGGAAGAGACTACATGAAAAATTCTTCATTTTCAAGTAAAGTAGGCCAAGAAATTGATGAACAAATAAGAAAAATTATTTTAACCGCCGAAAAGAAAGCACATGAAATAATTACAGAAAATCGTCAATTGTTAGAATTAATTAAAGACGCATTAATTATTAAAGAAACTATTGTTGCTGAAGAAATTGAATATATAGAAAAACATATGGAATTACCTCCTGAAATAACAAATACAAAAAAAGAACTTAAAAATGAATATTCAGAAGAAGATTTCAATGAATTATTTAATGAAGTTGCTGGACAAAAACAAATTCAAGAAGATAAATACAAAGCTACTTTAAACTCTGAATTAAAGAAACATAATATTGAAGATAATGATTCAAGCAACCATTCAGAAGATTCTAATAATACAAAAAATTCAGATGAAGACAGCAACAAAGAAGACAATCAATAAAATAAAAAAGTGGAATTCCACTTTTTTATATATCTCCGCTTTCTCGCAACATCTTTTCAAGGCCGTTAACAAGTTTCTTAGAAAAAACTCCTGTTCCTTTTTGCTTTTGTTCATATAAAAAATCTAATTCTTCATACAATTTATAAATTAATTTCAATGAATAATTGTATTGACTAATTGAATTTTTAAAATCTTGCCAATCTATAACGCTAAAATCTTTATCATCATAACTTTTAATATCGATATCAAAATCAATATATTTAATTACTTCATTTTCAATATAAAAAGGGCTTGCTAAATTAATATAAATATAGTTTTCTTTAGATTTATTTAATGTAATAGAAGCATTATAAAATTTGTTTCTAGCAAAGAAAAACAAAATAGGTTCAGAAACAACTCAATTGATAGATTTTTCAGAAACTTTTGTTTTCATTAACAAAACAACTACAAAGTCAGTTAAATTTGCAACTATTTTTGCCCCTTCATATTGTCTGTATAAAGTTCCATCATATTTAAAAACTTGAATGGTTGTTAGTTGTCCCATCTTAAAAAACTTTTTATGTTTTTTTATATTTTTTGAGTTTGGTGTATCAATTGTTTTTAATAAATCATCAAAACTCTTTATGCTTTCTTCTTCCATAATACTATTTAAATGGTATCAAATAAAAAACAAAAAGATTAATTAATTAAATTTTATTTTTAATATATTACAAATTTAACAACTTGCGATTTGCTGTATATGTTATAATAATTACGTGATTTAAACAAATTAAGCATTTTAGAATAAATCTATGTATGCTTTGAAAAGTAACCTTTGGTTGCTTTTTTTGTTTATTTAGTGTATTATAATTAATATGCGACTAAGATTTAATAAAAATGCAGAAACATCCTTAATGGCAAGCCCAATGACATTTAAGGATTTTCCTATTGATAATAAAAAAAATACTATATTAGAGATTGGTATGGGGAGAGGAACCATGCTTACAAAATTAGCCCTAATGCACCCTGACATAGAATATATCGGACTTGAAAAATACAGCACGCCCGCCTATTCTGCTTTAAAAAAAGCAATTGACCTCAACCTTGAAAATTTTCACATAATTATAGGAGATGCAATAAATTTATCAACTTATTTTAAAAACAAAATTAAAACTATTTGATTAACATTTTCAGATCCTTGACCTAAAAAAAGACATTATAAAAGAAGGTTAGTTTATAGAGATTTTTTAAAAATTTATCAAAACATTTTGGATAAAGACGGAGTGGTTTATTTTAAAACCGATAATGATATGCTTTATCAATTTGCAATAGATGAATTAAAAGAAATCAATGCAAAAATCATTTATCAAACTTCTGATTTACACCATTGCAATTTTGAAATAGAAAATGTTTTTACTGATTATGAAGAAAAATTTAACAAATTAAACAAAAATATAAACTTTATTGCGTTTACTTTTAATTAAAAAGGCCTGTAATAGAGGCCTTTTTTGTTATAATTATATAATAATATAATTATATTGTGTAAATGGAGTAAATTATGCTTTTTGGCGAAGATGATAACAATAAAAAAGAAAACAAAAATTTTGAAGAAGATAACAGCCACGATTTAGATGAATATACAAATTTTGATGATAGAATCAAAAAAATCTTTAGTGACGATGATGAAACAAAAGCAAAAAAAATAGACGAAGAGGACGAAGAAGAAATCCCTCAAGACAAAGAAGGCTATAAGGTAGAACCCCAACTTTTAGATAAAGAAATTAATGGTTTAAGACCCGCTAATTTATCAAAAGTAATGAAAACATCATTCATTGAATATGCAATGAGTGTCATAGTTTCACGTGCTCTACCTGATGCTCGTGATGGTCTAAAGCCAGTACACCGTAGAATTTTATATGGCATGAGCGAACTTGGAATGTTTTATACAGCGCCACATAAAAAATCAGCAAGAATCGTCGGAGATGTTTTAGGTAAATATCACCCACATGGAGATTCATCAGTATATGAAGCTATGGTAAGAATGGCTCAAGATTTTTCACTTCGCTATCCTTTAATTGATGGACATGGTAACTTTGGATCTGTTGACGGAGACGAAGCTGCTGCAATGCGTTATACAGAAGCTAGAATGAGTAAGATTGCTGGTGCAATGGTTGATGGTATTAAGAAAAATACTGTTGACTTTATGGACAACTATGATGCAACTGAAAAAGAACCAGTTGTTTTACCATCACGTTTCCCTAATTTATTAGTTTCTGGTTCAAGTGGTATTGCTGTTGGTATGGCAACAAATATTCCTCCTCATAACCTGGGCGAAACTATTGATGCAGTTTGTGCACTTGCAAAAAATCCAGAAATAAATGTTTTTGAATTAATGGAATATATTCAAGCTCCTGATTTTCCAACAGGTGGAATAATATTTAATAAAAAAGGATTAATTGAAGCTTATAGCACAGGTAGAGGATCAATCACCATTCGTTCAAAAGCACACATACAAGAACTTTCAAACGGAAAAAGTAAAATAATTGTTACTGAAATTCCATACGAAGTTAAAAAAACAGAAATCATGGAAAAAATTTCTGAACTTATTAAAGATAAAAAAATTGATGGAATTGCTGATTTTAGAGATGAATCGAACAGAGATGGCATTAGAATTGTAATTGATATCAAAAAATCTTTTGTTCCTGAAATTGTTTTAAATAACTTGTTCAAATTAACAAAATTACAAAGCAATTATTCATTCAATATGATTGCGCTGGTAAATAATGAACCAAAATTGTTGAACCTTAAGGCTTGCTTAGAAGTTTATTTAAAACACCAAATTGAAGTTGTAAGTAGAAGATTAAAATTTGATCTTGAAAAAGATAGAGCAAGAGCTCATATTTTAGAAGGTTTAAAAATCTGCATTGAAAATATTGATGCCGTAATTAAAATAATTAAAGAATCGAAAACTGACCAAGAAGCACAAAGCAAACTTTCTCAAGCATTTAATCTTAGTGAAATACAAACAAAAGCAATTGTTGATATGAAGCTAGGAAGATTAACTGGATTAGCAATTGAAAAAATGAACGAAGAACTAAATGAAGTACATGAAAGAATTGCTAACTACATTAAGATATTAGGCGACCATAACTTATTAATCAATTTGATAATTGAAGAATTACAAGAAATTAAAAATGCTTATGGCGATGCTCGTAGAAGCGATATAAATTGAGATGAATTGAGCAGCATAGACAATGAAGATTTGATTCCAAAACGTGATATTGTTATAACATTAACAAATAATGGCTATATTAAAAGACTTGATATTGAGGAATATAGAGAACAAAGAAGAGGCGGAGTTGGTGTATCAACTACAAAAACATATAAAGATGATGCAATTCAAGATATATTAGTTGCAAATACCCACACGGACTTGTTAATATTTACAGATCTTGCCAAGGTTTATAGAATTAGAGGTCACGAAGTTCCTGTTGGCACCAAACAATCAAAGGGTATTCCTATTGTTAATGTAATTCCTTCTATTCAAAAAAATGAAAAAGTTATTAAAATTTTGCCAATAAATGAATACAAACAAAATGAATATTTACTAACAGCAACTAAGAAGGGGATTGTTAAAAAAACTCCAACAATTTTATTTGAAAGTATTAACAGAAACGGAAAAATTGCCTTTGCCATTCGTGATGATGATGACAAATTAATTGATGCATTGATTGTTAAAGATGAGGATGAAATCTTTATTGCTGCCTCAAATAATAAAATTTGCCGTTTTGATTCAAACGAAATCAGGTCAATGGGAAGAATGGCCTATGGTGTTAAAGGAATTAAAATTGACGATGTTGAAGACAATGTTGTTTCTGTATCTTCTTCAAACCAAGGAAAATATATATTTAGTCTTGGAAAAGAAGGATATGGAAAATTGACACTTGCTGCAATGTTTAGAAAAACAAGGCGTGGAGCAAGCGGAGTTTATTCATTAAATGAAGAAAAATCTGGTAAATTAATTTATGCTGCAACTGTTCACGGAGTTGAAGATCTGATAGTTTTAACAAGCGATGGTGTTGCAATTAGATTTTCTTTAAAAGATGTTGCAATAACCGGTAGAAATACTAAAGGTGTTAAATTAGTAAATCTAAAAGGAAGAACCACAAGCATTGTTGCGGCAACAAAAATTTTAGATGATGATTTAGAAGACAATGACAGAAAATTATCAGAAAATGAATTCAAAGAAATTGAATCAGATGCTAAAAATAACCTTAATCAAAAAAATGAATAATTATTAATAACAAAATAAACTATAATTATTATGTATGCGTTAAAATGCATACAGATTGTAGTTGCGCAGAGAGGTTTTGAAAAATAATCTCTCTTTTTTTATATTTTAAAAACCTCCTTATAAAAGGAGATTTTTAAAATAAATTAAATGTTTAATTTTCTGGGTTTTGTTTTAATTCTGTAAGAAATTTCTCTAATTCTTTGTTTAATTCAATCGATTTCAATAGTCAAGGAGTTTTATCAACTCCCTTAAAATATTCAAACATTCCTTCAGGTGCATCTTTAAGTCCAAATCATTCAACTCATTTGTTGATAGATTCTAATTGATTTGAATTGTTGGGTAATTTTAACGTTAATTCGTCTAATTTTGTTTTTCATTCAGAAATTTTTGAGGCATATTTTCCACTTTGTTCTAAGCGTTTTATAGTATCATTAACTTCTTTGTATATTGATTTATTTGGATCAATTCTGGTATATGGTATAGAATAAGATCCACGTTTAAGGGTCGCTGTTGTGGCTTTGTGTTCTTTAAAGTTTGACAAAATCTTTTTTCAAACAACATCGCAATAAAATCATAATCTTTTTTCAACTTCTGACATTGTTTTATTAAAGTTAGACACATATTCATCAAAGGTCATATTTTTAAATAATATTTTCTTTTCTTTTGTTTCAACTACAGTGTTTTTAGATTTGTCAATTTCGTATTGGGATTGTTCAAATTTAACAGCATTTTTAGCAAATTTATTATTTCATTCGGATGAGATTCCTTCAAAATAAAGTCCATCAGTTTCTTTTAAAACATCACCACGAATATATGAAGCAGTATTTCAATCGTGAAATAATCAATTAATCCTATCAGTATTTATATTTCCATCAAATATTTTTATTAAATCTTTAACAAATTTTTCTTGGATTTCTTGTATATCATTTTCATATTTTGACGCTTCTGCAAGAGTTGCCAATTGACTAGAATTATTTTTGATATTTGCTAATTCTTGCAATGCCTTTTGTTTTATTTCATTTAGTTGTTGTGTTACACCTAAAAAATCTGGATCTTTTACACTACTATCTAGCTTATTAAATTTTTGCAAACTATTATTTATATCATCTAGACTATTATTTATTAATAATTTTTTAGCCTCAAGATATTCCACTGAAATTTCATTAATTACTTGCATTATTTCTTCTCTTGTGTGTAGAGTACTAATATTATGTTTCCTAATAGATCATTCTATTCTTTCAATGTATGCATTGTATTTATCTCTAGGGCGCATTTTATTTTCAATATATTCTTTTGCTTCTATAATTAAAGAAATTAATCTATCCCTTAATTTTTGCATATCATCTTCAACAAATTTTTTACTTTGTTTTAAAATTTCGTTCAAATGAATATTTGTAGCTACTATTTCTGATTTGTTAGAACTAATTGAAACGTTTGCTTGTCTTGCAACTTCTCCATTAAGATTTCTAACTATATCTGTATAATAATCAGATTTAACAACATCTTTGGCCAAAAATTCTTCAACTAATTTTCTAGTTTTTTGTAATTTTTCAAATTCTTGTTCTTTTGATTTTTTAAATTTGATCAATATATCTTCAACTTCAGAAATTTTAAAAGTTAATGATTTATTTAAATTATTTATTTCGTCTATATTATTTTTGTTATAAACCTCTAATGCATTAATAATTTGTGAATTTAAATCTTCCTTAGTTTTTTGAATATCATCATCATTATCAATTAATTTATTTTCCAATATCCTCATAACATGAATAATATTTTCCAACTGTTTCTTTGCTGCAAAAAGTAGTTTTTGCATGCTTTGCTTTTGAATTTTTGCATTTAATGAATCAATCGAACTTTTAATCTTAAAAATTTCTTGATTAAGATTATCAATTGGCGATTCGATATTGATCATTTTGGAATTATCAAAGACATTTTGTTCTTCAATTGAATAAATTCCTTGAATGTTTTTATTTTCTAGCAATAATTGCAATTCTTCTTGAAGAATTTTAAATTTTTTAAAGAGTGATTCTTTTTGTGTCTTTTCATCCACTTTATTTTGACACGATCTTATTGCAACGGTAGCGATGGCAGCTATTGATAATCCGCCACAAAAAATTGCTAATGGAAGAACGACCTTTTTGCTTTTATTGTTCATTTTTTTCTCCATTTTTTTATTATTTTTAATATTTGTAAAATTATTTTATGTTATTTAGTGCATAAGAATATTTTTATATTAAAATAGCAGGAATGAGATCATATAAATAGAAGAAAAGGTTTATTTTTTAAAAAATACATATATATTAAGCAAATAATTTATTATCGATTAATATGAATATATTTTACTTGTATAAAATAATATTCTTTGTCTAATCATAATTATTATTGGATAGAGTACGAAAATAACTGCGGTATATATTGGTATTTCAATAAGAGTTTTTGCTATTATTGGAATCATAAATATTGGATAATAAGTTTTGTAATCTCAATTACCTGATCTAAATCTATTATGATAATTTATATATGCAAATGGTCCTCAAAATCATCTACACAACATTAAAGTGAAAAACACAACCAATAAAATGCTAAATAAAAGTACAACATTGCTTTTTACAGAAAATTTTCTATTTTTTATAAATAAAGTTAAAAAGGTTATAGAAGATATTCAAATAAATGCTAATCCCACAATAGCAATAGATAAAACAATTCTGATTGGTATTAATTTGGTGCGTTTTACTGCTGTTTCATTTATTGGCAAATTGTTACCGTGAATTGCTAAAACTATTACAAAAATTGCTGTTATTATGCTTAAAAAACTAAATCCAATTATTCATGTAATCTTTTGTTTAGCATACATTAATCTTAATAATCAACCACTTATTAAAGCAATAAAAACAGGCACTAATGCATATTCAATCATTCATGTGCTTATTCCTCTAATAACCTGGTTTAGCGTATCACATAATATACCTAAAAATGCACCCTTTCATGGTCCCAATGCCAAGCCAAAAATAATGAATACTGCATAAGTAATATTAATATTCATTATTCCTTTAAAAACAAATCTTTCTAAGATTGCAGCAATTATATAAATAGCTAAAAGTAAAGCAAATAAAGAAATTTCAAAAACTGTTAATTTATGTTGTTTTGTTTTTTTAATACGTCTAATTTTTTGAATAAATTCAAACATGTTTCTCCTAAAATAAACCCTCAAAGGGTTTTAAATTTTGATAAATTATATTTTTAAATACGAGTTTATATATACGAGAACAGTTTTTTAAATCATAACTATTAAATTTTAACTATTTTTTAAAAAAATAAACAAAAATAAAAATTTCGGTTATTTGTCCGAAATTTTTAAACCTTATTTCAACACTAATTCTGTCAAAGTTTTTGTCATTGCAAACATTGGTTTTTCATCAATATCATTAGTTCCTGCAACATCTAAATGAATATATTCAATTCCCTTTGTAAATTCTTTTAAGAACATTGCTGCTGAACAAGAACCTGCATGTCCAGTATAGTCTGTATTCTTCAAATCTGCAACAATTGAACCCTTCATAAATTCTTCATAGTCAGAATCAAAAGGCATTCTTCATATCAACTCATGTTGAATGGTTGCTGCATCGCTAATTTCTTTTCATGCTTTTTCAGTTGTTGATCAAATGCCTGTATATGTGTCTCCTAGGGCTGCAACCATTGCACCAGTTAATGTGGCAACATCAATTAATCTTGTTGCTTTTAATACATCAGCTCCATAGTACAAGCCATCAGCCATAACTAATCTACCTTCAGCGTCAGTATTATTTATTTCAACAGTCTTTCCGCTCATTGAGGTCCAAACTGAATCAGGCAACGATGCATCTCCATTAACTCTATTGTCAGTAATGCACATAATTGCTGATACATTTGTTTTTGGTTTTAATTGGGCAACAGATTTCAAAACGGATGCAACTATTGCAGAACCTGACATGTCATATTTCATTCCTAACATGTTGCGGCCTGTTTTAATGTTGTAACCGCCAGAGTCAAAGGTAATTCCTTTACCAATTAAAACTGTTTTTTGATTTGAATCAGGATTTCCTTTATATTCAATTATTACAACTCTAGGTTCATAAACTGAACCTCTATTCACAGATAGTAATAATCCCATACCCAATTTTTCAATTTCTTTCTTTTCAAGAACTTTAACTGATAGGTTTTTATATTCTTTAAAGTCTTCAACAACTATTTTAGCTAATTGTTCAGAATTTAATTCATTAGGAGGGGTAATACCCAAATTTCTTGCTCAGTTTTGAGATTCAATTAATATTTTTGCTTTATTGAATGAGTCAAGTACTGATTGAGACGCTTGTTCTATAAACAATTCTATGTTGTTTGGATTTTCTTTATATGTAAATGTCTTTTTATTGTATAACTTTGCAGCTGAGAAATTTATTCCTTTTGTTAATGCATCAACTACATCATTAATTGTTAAATTTTCTGTTACAAAAGAAGAAAGATCAATTTGATAATCGTAGGCAGCATTCAATGCTAAATCTTGTGCCAATGAATATAAAGAATTGTATTGTAATTCTTGTTTTTTGCCTAGATAAATATACGAAACTTTTTTATCAATAAAATCTGTTATTGCTTCTGTTTCTTTAACTAAAAATTCTGGCAAAATTTCATCTTCAAATATTGCTTTCAATAACAAATGATCATTGCGCTTTGTTGATAATTGTTTAATTAATTGCATACTATTCTCCTTATTATTTTTGATTTGCTGCTAATTCAACAAAGGTAGAAACTAAAATTCCTAGTCCTACTCCTTTATTGTCAGCGGTTCCTGCAATATCGCAATGAATATAGTCAACATTATTTGTAAAATGTTTTAAGAACATTGCTGCTGTATTACAATCTGATTTTTCACTGTTGTTGTAATTATTAAGATCAGCTACTTTAGATTCTTTATTTGGTTTATCATAATCATCATGTAGAGGCATTCTTCAAACCTTTTCATGAGCAATTTTTGCCGCTTTGTTGAAATCTTCTCATCTTTGTTCAGAAGTTGATCAAATTCCTGAATACGTCTTTCCTAATGCTCTTTCCATCGAACCGGTTAAAGTTGCGGCATCAATCAATAATGAAGCATTCAATTTCGAGGCACCGTAGTATAAACCATCAGCCAACACTAATCTTCCTTCAGCATCAGTATCAGTAATTTCAACTGTTAAACCACTCATTGATTTGATTACAGATTCAGGCATTGTTGGGTGTGTATCTATTGCGTTATCTGTAATCATCATTATTGCTGCTACGTTTGCTTTTAATTTTAGTTGAGCAATTGCTTTAACTGCATATGCAACAATAACAGAACCAGACATGTCAAATTTCATGCCATCCATGTAGTATCCTTTGGTGTTATATCCTCCGGTATCAAATGTAATTCCTTTTCCTACATAAACATATTTTTCTTTACTTTCAGGATTGCCATTATATTCAGCAATTAAAACTCTTGGTTCATAAGAAGAACCCGAATTTACTGCCAACATAAGTCCCATGTTATTTTTCTTGATGTTATCACGATCTAAAACTTTTATTGATAGTCCAGGAATATTTTCTAAATCTTCTTTTACTACCTTAGCGATATATTCACTAGTTGCGACATTAGGGGGTGTCATTTGCAAACTGCGGGCTTTATTTGTTGCTTCAAGAATTATTGACAATCTTTCAACTAATTCTTCATACGATTTATCAGCATAAAACAATGTTATTGTCTTTTCTTGTTTGTTTTCTTTAGTTTTTGCTGAATAAAGATTTGCATTTTTAAATGCTAATTTAGATACAAATGCTCTAAAAACTTCTTCTGTTGACACAACATTTTCTTTAACAAAAGAAGCAATATTAATTTGATAATTTCTTCTATTTGAACTTAGAATTTTGTCAACTACTTTTAAAAAACTAAAGTAGTTATCTACAGTTTTTGGTATGTAAATAAATGCTTCATTTTTTTTAAAATCTTCTGTAATATGATTATCTTTCTTGGCAATATTTAAACTAGCGTTTGAGCCTTCATAAACTGCTGTTAAAAGCATATCTTTATTACGTTTAGTTTCATAATAAATAGAATTCATTTTATTCCTTTCTAATTTTTAAATAAATATCTTTTAGATAATTAAATTATAATGATTAATATTTTTTAGAACGTAAAAACATAAAATAAAGAGAAAATTTTCAATATTTTTAATTAAATTAAAAATAATAAAAAATCAGCCTTACTAAAAGCTAATTTTATTATTTAATATTTTTAAGTTGATTAATAACTAAATCTATGCTAAATAAGATTGCGTCGCATTGTTGATCATTTAGTTTTGCAATTTCTTCATCTTTTATCATTTTTTCATATTGGTTCATAATTTCACCAGCAGATGGTATCTTTTTTGCCATATCAAGTAATTGCTTTATTTTTGATTTAACTTGTTGTTGCTTTGATGTCAATTTTGAACACGATATTGCTACTATTGGTAATGAAATTAAAGAACTTGCTCCTAAAATAGACAAAATTATTTTTTTACTCTTCTTCATAGAATATCCTTTATCTTTGTTTAACGAATTATTAGATATGATAATTATAAATATAAATTTAATTATTTTTTAGTAGATCAATCGATTTCATCTTTTGATAATGATTTAAGTTTAAAATTTATTTTTTTAAAAACATTTGAACCAAAAAATCCTTTATATGCAGCAAGAGGTGAAGGATGTGGCGCATAAACTATTATTTGTTTAGACATATCAATGTTTTTTATAAAATCTTTGGCGTTATTCCCTAAAATTAAATAAATAATATTGCTATAACACGAATTAATTTCTGTTAATAAATTTTGATTGAATAGTTCTCAACCTAAATTCTTATGTGCCAATGTCTTTCCTTCAATGTTTGTTAAAATTGAATTTAAAAGTAATACACCTTGGTCTTTTCAATAATTTAGCGAAAAAGTTTCAAATGTTGAATTTGGATATTCATTTTTTATTTCAATAAATATATTTTTTAAAGATGCCGGGCATTTTGTTGCATTGCTTGAAAAACACATACCATCTGCGTCACCTTTGTTGGGATAAGGATCTTGGCCAATAATAATAACCTTTAGGTTGTCAAAATCGAAATTTTTAAATGCAAAAAATACTCTGTTTGGCTTTGGCGTTACATTAGGCAACATAGTTAATTTTTTTAAGACTTCAAAATATGGTTTTGAAGTTTCTTTTTTTAAAAATTCTAAAAAAGCAAATTTTGACATTTTTAAACTCTTACTCTTTTTTTCCCTTCAAATTGATATTCTATTAAGTACAAATATCCATCATTAAATTTTATTTTCAATGGGGTACTAATTTTTGTTGTTGAAGCCCGGAAGATTTTTAATCTTTTATTTTGAAAAATTAAATATGCACCTGGTTCATCATTAAAAGCTCTGATTTTGTTTACAACACTTGAAAGATTTTCGCTTTGATCAATCCTTTCCATACCATTTAATATCTTAGGAGCAAATGTTACTTTAGATTCATCCTGAGCCACTCGATTATATTGGCCGTTGTAAATTTCAAACAATCAATTACCAAGATTTTGCGAGGCCGCATTTGCTAATTTATCAAATACTTCAATTGCTGTATCTGTAGAATCAATTTTCACTTTCGAGGATTCTAGGATATCTCCTGCATCCATTTGTTTTACCATTTCAATTAATGTTATTCCTGTTTCAATATCACCATTGAGTAATGCATATTGCACAGGCGCAGCTCCACGATATTTTTCAAGCAAACTTCCGTGAACGTTCAAGGGCATTTTTTTAGGTAATTTTAATATTGAATCAGGTATAAATTGTCCAAATGAAGCTGTGATAAAAAAGTCAAAATCCATTGCGCTAAGTTCGGGCAAAATTTCTTTTATTTTTTGAGGTTGAAAAACTTTTATATCATACTGTTTGGCAAGTTTAAAAATTTCTGTTTCAATTACATTTTTGTTTCGATCCAATTTTCTATTTGGCTGAGAAATAAATGCCAAAACATTGAACCTTTCATCATTTAAAAGAGGTTCAAAAGTTTTAGCAGAAAATTGTCCAGTTCCCGCTAGAACTAAATTAATTTTGTTATTTATATTTTTCATATCAAAATTATAAAATAAATTATTTTTTATAAACTTATTATCTTGTATTTCAAGCAAATGTTTTTATTGACAATAAACTCAATATTACAATTCAAATTCAAGGCAATATGAAGTTCAATAAATTTTCTGTTTTTGAACCTATTTTTGTTAAACTATCATTGCTTAAAACGTTATTTGAAAATTGAATGTAACTACTGTCTAAAAATTTAAAATAGTTTGCAAATTCATTATTCTTTAACAATTGGTCCAAACCATTTTGATTGAATGTTTCAGCAATGGTTGAGTATTTTAAAATTATAGGTTGATAAACATTTAAAATATTTAGTGTTTTATATTCGTTGTTAATATTAAATATATTATATTGACTATATTTTATTCCAATTGGTGTATTGGCTGGCAATGCATTATTATTTGCTAGAAATAAATCTTTTTGTTGATTCAAGAATTTCAAAATTGTTAACCTTGCAATTAGATCTTTGCTATTTGCTTCTTTCAATAAATTTGCATATTTTATAACACCTTCAATGTATTTTTGAAGTTCTTCAATTGTATAGATTTTATTGCTTGAATCAGCACTTAAAAAAGGTTTTAATAAATCTATATTTTTTATTTCATTTCCGATGTTAAGAGTTGCCTTTATTGAAGCAATATTATACTCATTTGATAAGGATGCTTGTCCTTGAAAAGAAATTGTGTTTTGAATTATTGCACTCTTAAAGGGCGTTAAGTAGGAAACGTATCACATTCCTTTTATATTTGAAATTTGAGAAATAGGCAACACTTGGCCCGATAAGAACATTGTGATTATTAATAGCGAACAACCTATTGCTTGAATAGTTAATACTGATTTTGAAACGCTTATAATGAACAATCCAACAGATATGCTTACAAAAATTATAATAACAAAACTAAATATATAACCTGGCCAAAATATCCTTGAGAACATTTCTTTAATACTACTTCCATATAAAGTTATATTTAGACTTGCTTCTCCAATTTTTATATTATTAATTGTTCCTAAAGAACGACCGGTTTTAAAATATTCGCAAAAAAACGTTAAGGCAACCAGCATTGTTCAAAGGCCAGAACAAATCATTATAATCAAATAAAATATTGCTGTATATGCCAAGAACGATTTAGGCCTAATGCTAGAAGCACCAATTCTTTTTAAAATTGTTGATTTTTTAAACTCAAATATTGCCGTTGGCAGTGAAATACAGGCTATTGCCACCGGCCCAATGGTAATTGTACTTCCTAAAATCATTTCATAACCAAATATCGTTCCTAAAATAGCAACAAAAATAATTGGATAGCCGAATGCAAAAAATGGTCCTACAAATGATTTTCAAAAATGTTTATTAATAATTGCTAATATTCCTAAAAACTTATTTTTGTTAGCCTCGGTATTATTGTTTTTTTTAATAATTTCTTTATTTTTCATAATTTTAAATATATTCCTTAAGACATTGAGATATTGATCCATATTTTTCAATTATTTCGCTTTTCAAAGCATCAAGGACAATTCTACCTTTATTCATAATAATAATTCTATCTGCCAGAATTTCAATTTCATTTATATCGTGAGATATTAAAATCAAAGTACAATCAATTTCTTTGATATAGTCATTCAAAAATGATACCAATCTTGTTTTTATTTTTATGTCAAGACCAGTGCTTAATTCATCTAATATTAACAACTTTGGTTTATTAATTATTGCAAGCAATGCATTAAGTCTTTGATGTTGGCCCCCGCTTAAGGAAGAAGCTTTATTATTTATAAATTCATCAACCCCAAAAATCTTTATAAGTTTTTGCAATTCTTCTGGTGAACTTTTATTGCCATAGATTTTGTTCATTGAATAGATGACATCATTAACCGATAAACCTTGAGGATATGAAGAATCCTGAAATTGAATTCCTATGTTTGTTTGTGCACTTTCATTATGATTTGATCATCCATCATTAAAATAATATTCAATATTGCCTGAGGTAGGTTTTAAAATTCCTATAAGCATTTCAACTGTTGTAGTTTTTCCGGCTCCATTTCCACCTAATAGAGCAATGTTTTGATTCTTATAAATTTCGAGATTCAAATTATCTACAACTTTTATTTTTTCTTTTTTTATTTTGAATTCTTTTGTCAAATTTTTAATTTTCACCAACTTTTTTAAATTGTATTGGCGCATTTCAATTGAATTTGCTTTAATTGTGTTTTTTTCATGATCTTCTAAATTACTTAAAAAATTGTCTATTATTTCTAGACTTTTATTTTTACAAACTTTTTCATTTTCCACTATTTTAACTCCTTATTTTATATATTTCCCTTTTTGCCTAAATTATATCAATAACTTAAAGAATGAAAACAAAAACTCGTAATCAAAGAATTACGAGTCAATGTTTTTATGCTTTTTTTGTTTTGCTAAAAATTTTTGGTTCTGTATGTTTAAATATTCGTTTAAAATTGGGTATATGTTTAACAACTATTATTAGCGAAGCCAATATTAAAATAATTACATGTGCGGGATATATAGCATTTTGTTGCATATAACCAAATATTGTTCCTGCATAAAATATATAAATTAAACCTAAATAAGGAATTATTCCGCAGCTTATTGATGAAGCAAGTGAAACATATTTTGTTGAAAAAACTAGTATTACATATATTGCTACAAAAATTAAAAATAATAGAAAATTATATGCAAGCATTATTCCAAAAAAGCAAGCTACTCCTTTGCCGCCTTTAAATTTAAAATAAATTGGAAAGACGTGTCCAATTACTGCACCAAGCCCTGCTAGCATTGGAAAAATATATTCAAGATTTCTTAGATATTTTTTAATTAAAAATGCAATTATTGTTGCTAAAAAGGCTTTAAAAATGTCAAAAGCAAAAACTAATGCTGCAAATTTTATTCCAAAATTTCTTAATGCGTTTGTTGCTCCAGGATTTTGCGAGCCAATTTTTCTTATATCATTCCCTCTTAATTTAGTTATTATTATTGCCAAATTAAAAGAGCCAATTAAATATCCTAATATTAAAAATATTATATTTAATCACACATATTCACCATAAAACATAAATAAATGATACACACAAGAGAAATTATTTTATAAAAACTTACAAAAAAAATAGCAAAACTCTATTTTTGTTTGCTATTTTTCGGTCTTTGTTTCAATGTTAATTATATTGCTTGCAACAACATCACTACCAACTAAAATATCATAATGAGCAGATATCTTGTTTCCTTCAATAAAAACATCTTTTAAATAAAAATCTAGAGAAAATTCTTGAATTTTATTGCTTACTCTTTTGTTTTTTTGTAACTCGATATATGAACCAGAATATTTTATTTTTAAAGTATCACTATTGACCAATAAATCACATTCCTGATTATTATTATCAATAAAATTTATATGAATAAATTCATCAACAACTTCTTCAGTATATGGAACAAATTCTGTTTCTATCGTTTTAGAGGGTTCATTATTGAATTCGTTTTTTGTAATTAAACTATATTTTAATAATTCCATTTAATCTCTTAAAAACTAATTCAGCCCCAAAAAGATAGATTGCTTTTGCAAGTTCAGGGCCATGTTCCTCAAATGTTGTTGCCTTTCTAATTGGCATGAACAAATCTCTTCCCTTAACATTCAAGATGGTTTTTGTTTCATCAATTGCTTTTTGTATATTTTCTATATTGAATTCTCTTGAAATTAACTCTTTATAAAAAACTTTTACAACATCTAATCTTTCTAACTCTAAGTCGCAATTTTCTTTTGGGTTTAAATAAATTTCTAAATCTTTCTTTAATGTTGAAATCGTTGGACTTGATTGTTTATAAGTTTCAACAAATAATTCATTTCATTCATGTTCTTTTGGAGAGTTTATTTTTTTAATTAACTCTTCATTAGAAATATTTTTCATATATTGCTTTGCAAATCAATCCATTTTTATAATGTCAAATTTAGATGGTGATTTGCTCAATCTTTCTGGATCAAATTTTGCAATTAATTCTTCTCTATTCATTAATTCTTGCTTATCTCCGGAAGTTCAACCTAATAAGGCTAAAAAATTCATTATTGCTGCTGCGGGATAGCCTTCATTTTTATAGTCTTCAATAAATTGTTTTAAAGTTTTATCTCTTTTTGAAAGCTTTTTTCCTTCCATATTCGTAATAATAGTTAAATGTCCAAATAATGGGGGTTCTCATCCAAATGCATCGTATATTGCTAATTGCTTTGGCGTGTTTGTGATGTGTTCTTCGCCTCTTAGTACATGTGAAATTTCCATTTGGTGGTCATCAACAACAACTGCAAAATTATATGTTGGATAACCATCACTTTTCATAATAACAAAATCTCCTATATCATCACTATTAACTGAGATTTTGCCTCTTACTAAGTCATTCCAAGCATAATCTTTATTTGCTGGTAATTTTAATCTTATTGAATATTCTTTAGCTTCTTCTCTTCTTTGCTTTTCTTCATTAGAAATTTGCAATCAATTTGGATTATAACGAAATGACGCAACGCCAGCTTCTTCTGATTGTTTATGTTGCATTGCTAATTCTTCCTCTGAATCGTATGCTTTATAAGCGTGTCCATTTTGAATTAGTTCTTCAACAAGTTTTTGATAAATTGGTAATTTTTCGCTTTGACGATATGCACCATATTTTGAATTAGGTTTTAAAGGACTTTCGTCAGGGATGATACCAAGTCACGCTAAATTATTTAATTGGCTTTCTTCACCTCCAACAACGTTTCTAGCAACATCAGTATCTTCTAGCCTAAAAACAAAAGTGCCATTGAAATGTTTTGCAAATAAATAGTTAAATAATGCAGTTCTTGCACCACCAATGTGCAAATACCCAGTTGGGCTCGGTGCATATCTTGTTCTTATAGTTTTCATAATCTACCTTCTTTTATGGCTTTTCAAAAATAGCAGTTATGGGTTCTGAAGCATTGCTTCAAAACGCATCCTTATTTCCCTTCATAATTCCTTTATAAAAAGGTCAAGGTGCTTCCTCAATAGAAGTATTAACATTTCTATTTTTTCCCTTAGCTAACAATGATTCAATACTTGGATCTAGGAAATCTGATTTTCAATTAGGGGCTGCTTCATTTTGTTTTAAAGTTCAATATAAAACTAAAAATAATGCTCTTGGATTATTTCGATAAGGTCAGTATCTGGAATCATTTTTTGTAGCAGGAAAATAATTAATTAATTCCTCTCATGTAAAAGAGAAAACTCTAAAATCGTAATTTGGAGAAATATTATTAAAATCTAAAAATCTGCTAAAAAATTCTCTATAAACTCTTTGCACTGGTTTTCCGTCAATTACTTCGAGTTCATTATTCAATACTTCTTTCAATCTTGGATATCCATTTTCCTTGTCATAAATGTAGTTTCCGTTGGCATCTCTATCAAATTCTATTTCACCATTTTCCAATTTTTTACCCTTAAATTTTAATAAATTTCCGTTGGCATCTCTAAAATAAATTGGATTTCCTTTTTCATCTTTTAAATTTTTTGTTAATTTGTACAAGGAATCTCTTGGCGATATCGGGTTACTTACTGAATAATTATTTTTATTAAAATCAAGTGCAGAACCCTTAACGTGATAACCATATTTTTCAAATGATTCTTTCGCTTTTGAATTGAGAGTTATGGTTGCTGTTTTATTACAGCTTGCCGCAACTAGTGGCAATGCAAACACAGGAGCAACACTTCCTATACAAAGTAAAAATTTTGTCATAATTGCTTGTTTTTTCATGTTTAAAATTATACAAGTTTTTTTAATATATTAATAACCATAAAAATAAAGTTTCTCTAACTTTTCGCCATTTCAAAAAAAATAAACTTTTTCAATCAATAATTTAAATATGTGAATAAATAAACAAGACAGCGTTAAAGATTGTGCAATTTATACATTGCAAGCAATGATTTGCTACTTTTTTAAATATAAGGCAGATATAAATTTTTTAAAGAAAAATACGATATACGACGAAGAAGGAGTGTCGCTATATTCATTTAAAAAAATTTCTGATTTTGTAGGGTTTGATACTAATATTTACAAAGTAGATTTTTTAAGTTTTATTTCAAATTTGAATGATATTTCATTTCCGTTTGTCACAATATTGAAAAATGATAATTTCTTGCATTATACAATTGTTAAAAGAATTGATAAAGCCTTTGTTTATCTATGTGATTCTGAATTTTTGAATTATAGATTAGAAATTAATGAATTTAAAAATAAATTCATAAATGTTGTAGTTATATTAAGTAAAAAAATTAAAACCCCAAATTGCACAAAAATTAACTATAATCTATATCTAACACAAAACACAAAGTTAACACTAATTAGTATATTTTTGGCAATTTTATTCAATGCTCTTATGCTTTCTTCTTCATTTTATATTAAATATGTTTTAAGCGTTTTATGATCGGAAAACGCTAATAATAGATTGCTGTCAATATTAATTATTTTTAGTTGAATTGCAATATTAAAGGTAATTACTTGATTATTCAAAAAGATTATTATTAATAAGCTAACTTTTTATTATGAAAAAAAGTTATATTTGTTAGTCAAAGAAAGATTAAAATTTGCAAGTCAGAAAGACCTGCAAAAAATTAACCAAAATGATTTTATGGCAAGAATATTAAGTATTCCAACCATTGCAGAATATAAGGCGGGTTTATTTTTGTTTTTATTTGAAAATATAATTATTTTTATTAGTTCAACACTGCTTTTAGGTTTGTTTAATTATAGAATATTATTAATTTGCTTATTAGGCATTTCAATAACCTTTGCAATCTCGTTATTCAGTTTAAAATATCAATCAAAAAAAGCAACAGAATTAATAAATTTAAAGATAAAATCAATTAAAATATTCAATAATTATTTTTTAAATTATGAATATAGGACGAACGATTTAAATAATTCGAATTCTAAATTATTTCAATCATTATTTAACACTCAATATAATCTAGGAATATTCAATGAAACAAAAGATTCATTTATTACATTTATTTGAGATTTTATTCAATTAATCATTATATTTACTGGCGCTTGATTGTTTATAAAAAATAAAGATAATTTTACAAATATTACATTATATGGATCAATATCAATTTTTTTGAATTCCCCTATTGTAAATTTAGTCTTCCTGATTTCAAATTCTTCAATAAATAAACAGGGAATAATAAGAATAAAATATTTATTAGAAATCACACAAAACAAGGATGGAAATATATCATTCAAACCCCAGAAGCAAAATAAAATAAACATATTAAATGCTTCAATAAATATTGGTTCAAAAAACGTTATAAAAAATCTTAATTTAACAATCAAAAACAATCTAGAAATATCAGGAAAAAATGGAGTTGGTAAAAGTTTAATTTTGAATGCTATATTTAAAGTTTATTGATTAAATCAAGGCCACATTTTTGTCAATAATATAAATTTAAAGCATATTAGTCAAAAGTCATGATTAGATGCCTGTTTTTTAAATAAAAATAATGAATATTTTTGCTATGAAAATCTAATGGATTGCATATGTAAGAAAGACAATATAAAGGAAAATATTTTTCTTAAAAATTTCAAAAAATATAATTTAAATAACTTGTTTTTAGATATGAACTTACCTCTTAATAAATATATTGAAAATGGAGGAAATAACCTATCATCCGGCCAGAAGGAATTCATAATTATAATGCGACTTTTTTGCCAGAAATATTATTTTATTCTTTTGGATGAGGCTTTTGAAAACATTACAAACGAAATTTTTCTTAAATTAAAATTTTTAATTTTAAACTACCAAGATAGGGCCATTTTTATTGAAATTAGCCATAACAAAAAAATTGTTGCAAACAAGCGAAAAATTTTCGCTTTAAAAAAATAAATATTTTTTTGTTTTTATTTAGTTTAAAAAAGAATATTATCTTTATAATTAATAAAATATGAATAGAATTAATTTTAAAAACCAAAGCTTTCATCGTTTTAATTTTATGAAAGATTTCTTAGATATTTTAGAAGAAGCAAAAATAGAATTTGGTGCAAAAAAATCACTTTGTGTTGATGTAATGTTTGTAACTGCTAAAAAGATGAAAAAGTTAAATAACTTACATAGACAAAAAAACTATACAACTGATATTTTAAGTTTCCCACTAGATAGTCAAGAAGAATTAAATTTTTTGGATGAAATTGAATTAGGACAAATTATTATTTCTCCGTGGAAAATAAAAAAACAAGCCAAGGAGTTCAATCATAGTTTAAGACGAGAATTTTGTTATATTTTTGCTCATGGTATTGCTCACCTATTTGGTTTTGATCACATTACTGAAGAAGAAGCAAAAATAATGAATGGGCATGTAGATAACATAATGAATAAATTAAACATAAAGAGAATATAAAAATTGGAGAAAAATGAAAAAAGTTTGCAATGTATGCTTAATTGGGCGCCCAAATGTTGGAAAAAGCACGTTTTTAAATAATGTGTTAAATTTTAACCTATCAATAATTACGGATAAACCCCAAACAACAAAAGATAACATTAGAGGAATATACAATGATAAAGACTATCAAATCATTTTTATTGATACTCCAGGAATTCACAAAGCAGAAAATTTATTATCTGAAAGATTAAATAGTAAATCATATGAAGCTATTGAAAATTCTGATGTTGTTTTGTTTTTAACTCCCGCTAATGAAACAATCGGAACAGGAGATAAATTCATTATTGAAAAAATAAACGAAACTAATAATGAAAATAAAATTGCAGTTATTTCAAAAATTGATTTATTAAATGTAAAAGAAGAAGCAGACAAAAAAGCAGAAGAATTAAAAAAACTTGGATTTGAAAAAGTTTTTGGCATTGGACAAAATTTGCCTACTTCTTATTTAGATTTGATTAATGAAATTAAAACCTATTGCTACGAAGATGAGCCATTGTACCCTGATGATCAAATTGGGGATATAACATTAAGGTTTATGGCAAAAGAAATAATTAGAGAAACTGCTATAAACAATTTAAAATTTGAATTACCACACTCAATTGCGGTTGAGATCGAACAATTTGATGATAAAAACGAAGATGAAGATTACAAGATCTATGCTTTAATTTATGTTAAAAAGAAGTCTCAGAAACCTATTTTAATTGGAAAAGACGGTCAAATGATTAAAAAAATCTCTATTGATTCACGACATAAAATGGAAAAAATTTTTAATCACAAAGTCTTTTTAAATATTAAAGTAAAAATTAGCGAAGATTGAGTAGATAATGAATTCAAAATAAAACAGTTAGGATATTAAGGTGAAACGATTAACTACACATAGAACGAGAGTTATTTTTATAGCAATAATTGCCTTACTAACACTTGGATTATTTTTAATAATTTTATTTTTTAAAATTAGCTCCCCATTTAAAGTAAGTATATATAACTACGAATCATACTTAGATAAAAAAATAATTACTAAATTAAAACAAAATTATTCTTATCACGCCTTTACAAATCTAGATGAGTTTACGAGAGCAATAAATAACGAAAAAGCCGTTGCCGGAGTTGGAAGTGATTACCAAATTGCTCAACTAATTCTTGAAAAAAAATTAAGAAAAATTAACTTTGCAAAATTGTTTAATAATTCAAAATTTAATGATTCAACCCAAATAAAGAATCTTTATCCAGAAATTGTTAATAAACATTTTGATTTATTTGAAAAATTTATCATTAAAAAAATAAGACAAATAAATCCTGAGAACTTCCAGCAAACTGATTGGCAAAATATTAGTCACACCAAATTCTTACCTTTTATTTATTATGACAAAAATAACAATATTTTAGGTTTTGAAATTGATCAAAAAAAGGGAATTGACCACTTTTATGAATTTTTAATTCCTTATTTTATAATGGATAAAATGGTTGTTTATAATACAGATAAAACCCCAAATAAATTTACTGAAAGAAGCAATTTAAAATCTAATGAAAACTTTAACGATTTAGAAAGTTTAAATTCTTGAAAAGATATCATTAAGACACTTGTTTCTAAATATAAAAACCCTAGAGTATATTGAACAAATTGATTTCAAGATAATGCTATGATTGGCCAATTTTATGGTTATGAATCTCTTAACAAATTAAAATATTTTTCTAAGCAAAACGAATGGGCAGATATAAATCACGAAAATTTTAAAGAGATTATTAATGATTGGGTTTCTTTAGTTAAGGATGCAACAGGAGAAAGTATAAAAAATTCTAAAACAAATAAATTAGCTACTGATGGACAAGAGTTGGTTAGTTCAATAATAGAGCCGATTAATGGCAAGGCAGATGTTTCAATTATGTATAACGGAGATGCTTTAGATGCTTATTACGGGGAAGACAATTTTAAAAAATTAGGTAATATTCCACATATTGGTTTTAAAAGGCCAAAAAATACTTATATGAATATTGATGCTTGAATAATTTCAAGAGAAACATCTGAAAATGATGCTAATAATTTATTAAAAATATTATATGAAAATGTTATAAAGGGAGCTGAATATTCACTCCAAGATATTGAAACAAAATATTTGAAAGAAGTGTTGAATCTAATATCAAAAGACAACAAATTAAAAAAAGATGAAATTGTTAATGAGCTATTTGCAGACAAAAACATGTCTATGCCCAAAAAAATTAAAGACATTAAAAAAGATTTTTTTGCAGATTATTTTGACTATTTCAAAGAAGCATTTGAAACAATGAATTTACCCATTGTAAACAATTTTCATTCAATAAATTACACACCAGGCTTTAAAAATGTAAAAAATTTTATTGAAAAATGGTACTTTTTGAATAAAAATCAGGAAGTTGATAAAACTGCTTTGGAGATATTCAATCCAGAATTTAATAAAAAAATCAATCACAGAATTTACCAACCACTTGATTTAAATTTAAAAACAAAAATAATAGATTATTACTTTCAAAAAACAAAATCATAATATTATTTAATTTCATGTTAGGAGAAAAATGGAAAATAAAATTAAAACACCAAACAAAAAAGAAGAACCAATAATTCAATTAGTAGACGTGGTTAAAGAATTTGAAGATAAAACAGTTTTAGATAATGTTAACTTGTCAATAAATAAAGGTGAATTTATAACATTACTAGGTCCGTCAGGTTCAGGAAAAACAACCATATTGAGAATTATTGGCGGTTTTGAATGAGTTACCAGAGGTGAGGTTAAATTTTTTGGCAAAGATATCAAAGACTTAAGTCCACATAAAAGGGATGTTTCCACAATTTTTCAAGATTATGCATTATTCCCTCATTTAAATGTAGAAAACAACATCAAATATGGTCTTAGATTAAAGAGAATACCAAAAGATGATATCCCTAAAAGCTTTTACAATAAATTAAAATATCAACAGAAAAAATGAACAAAAAAAGCTTCTATTGAAATGAAAAAGCTTGATAAAACAATGGCGCAATATGAAAAGATTCTTGAAAACCAAAAACTTCCCGAAAAGAAACGAAACAAATACCAATCTTGAATTGATGACATGGATTTCAAATACTCATATTGAGAAAATTTTAAAGATTTAAAAACTGAAAATTATGAAAAAAGATATCTAACAAGAAAAATAACAAATAACGAAATGAACCAAGAAGTATCGGAAATCATGGATCTTGTTGGTTTGAAGGGAAATGAAAAGAAAAATGTTAATTTCCTATCTGGTGGTATGAAACAACGTGTTGCACTTGCTCGCTCTCTTGTTATTGAACCCGAAATTCTTCTACTAGATGAACCATTAAGTGCCTTAGATGCTAAAATTAGACAAAAAATGCAAACATTATTAAGGGATATTCAAAAAGAATTAAAAATTACGTTTATTTTTGTTACTCACGATAGAAATGAAGCATTGCAATTAAGTGATAGAATCGCCATTATCAGAGATGGTAAGGTTGAACAATTTGCTTCACCAAAAGATTTATACGATTATCCAATAAACAAATGAGTAGCAAATTTTATAGGCGAATCTAATTTCTTTGATGCTGTATTTATTGATGAAAATAAAGTTAAATTTATGGGCAAAATTTTTGATACTATTCACAAAGAATTTGAGCCAGGAGAAAACCTTGATGCATTAATTAGACCCGAAGATGTTTATGTTACCAAAGTAAAAGCCAAATCTAAATTTATCGGCAAAATAATTAAATCAACATATAGTGGTAGTTACTACAATTATGAGGTTTTAGTAAAAAATAAAATAATAAAAATGGAAACATCAATAAACTATGAAGTGGGTAAGGAAGTATATTTAGATTGATACAAGGATTCAATTCATTTGATGAAAAAGGATATTAAATTTGCCAAGGAACTAGCAGAACAAAACGAGATTAACAATGGAAAAACAACTGAAATCAACTTCTTATAAAGAAATAGACAAAAAAACTAAAAAAAGTAGTAAATTTGCAAAATTCTTTTTGGGTGCAAACAAATTACAACTTTCTTTGCTGATTCCTTATTTAGTATTTTCAATTTTATTTATTATTTTGCCATTAATTTTGATATTTATTTATGCATTCAAACCACTAGAAGGACAAGGTCATCTTGCAAACTGGGAAATAGCAGGCAAATCATCAACCTGAATGATTATTCTTAGATCAATTGGAACCGGAATATGTGCAGCGTTTTTTGCATTAATCATTGGTTTTCCATATGCCTATATTATTGCAAGAAGTAAATCAATGATATTTAGAACTTTAGGATTAACATTGATTCTAAGCCCGCTTGCAATATTCACAATTTCAAAAGCTTTAGCAGTTAGAGGTTTATTTTCGGCTATATTTGACGAAAATGAATTAAATAATAATTTCTTTCTAATCTTTGGAATGGTGTATTTGTATATCCCTTTTATGGTAATGCCATTATATCAAGTTATTAAGGATATGCCTAACAATATAATTGAAGCATCTCAAGATTTGGGATATTCAAAAACACAAACTGTTTTTAAAGTTATATTGCCTTACTGCATGAAAGCTATATTAAGCGGTTTTGGAGTTGTTCTTATGATGTCAGCAACATCAATAATTCTTTCAGATAAATTGTTGCCAAATGGTAGTCAAAAGCAGCTTATAGGAAATCTTATAAATCAGTTTGCTAATACAGCAAACCCTTACGACTTAGCAAATGCCTCTACATTAGTTATTATCACTTTGGCTGTTTTATTAACAATATATGGAATTATTTATGGTATACCTTATTTAATTTCAAAAAGAAAGCAAGGAGGAATATATGAATAAATTTAAAAAATTTTTGAAGTATTCTTATGTATTAATAATTCTTGCTTGTTTATATATTCCAATCATTTTTGGAACAATATATAGCTTCAACGCACCTTCTGACAAGGGAATATTTTCTGTAACCTCATGAAATAGAACTTCTTATGAAGCATATGTGGAACTTTTTTCAAAATCACATTGACTTGCATTTGTAAATTCTTTTTTACTAGGAATTTCAACATCTATTTTTGTTATATTGCTTTCATTGATGACGGTTTTTGCACTTTGAAGGCATAAAAACAAAACAGTTAGAGCATATGTACAATCAACATCAAATATTCCTTTAATTAATCCAGATGTTATTACTGGTTTAACGTTAGCAATTGTTTTAAATCTTATATTTTTTACCACACTAAAAGCAACCAACGAAGGATTTTTTAGAGCAATAATTTCACATACAGTAATGACTCTTCCTTATGGAATATTAATTATGTTGCCAAAAAGTGATAAATTTTCAAAAAATATCTTTGAAGCTAGCCAAGATCTAGGTTATTCAAAATTTAAAACCTGATTTAAAACTTATTTTGTTTATATGTTAGGTTCAATAGGATTTACATTTGTAATCACAATGACACTTTCTTTTGATGATTTCATTATTACTAGAATTGTTTCAAATACAGAAACTTTGGGTACTCAATTATATGAAGGCCAATTTCAAGCATGATCGTTAGCTATTGGTGGAATATCATTACTAGTAGTTTTAATGGCCAATACAATATATGTAGCCATTAAATCTTCACAATTTTCACATAAAAAAATATCATCAATCAATATTTCTTCAATAAAAAAATAATATAGGAGGTAATTTTATAAATTGAGATCACGAATTTTAAAAGCTATTAGTTTAATATTTTTACCCTTGATTTTTATTGCATTATTCCTTGCTTTTTTAGTTATAAAATCAACTAATCAATATAGACCTAGCATCTATAACTATGAATCTTATTTAGCTCCAACCATTATTAATAAATTAAAATCAAAATACAATTTCAAAGAATTTAAAGAAATTAGTGAGTTTACTCAAGCATTATTACAAGATAAGGCTATTGCTGGAATTGGAAGCGACTTTCAAGCTGCTCAACTTATTTTAGATAGGAAGATTCAAAAAATAGATTTTAGTCTAGTTTATGGTGAAAAATACAAAGATTGAAAAGAAAGACAATTTCTATATACCCCAAATGTTAGAGAGCATATTTTAAGATTTGATGCTCTGATTTTAAATATTTTAGAATCTGATAAATCTAATAAATATAAAAACTTCGAAATAAAGAAAAATAAAGAAGGCAAACCTATAGCATTTAGAGACAAATTAAACAATAACAGCAAGGAAGTTTGGGATCATTTTTATGATTATATCATTCCTTATTTTACCCAAGATAAAGGTATAGCTTACAATATAAATCCAAAGACTAGACCAAATTTAAATATAAACAACGCTATAGAAAAGCTAGATAAAAACGACAAGATTTTTTCTTGAAATGAAATCTTGCAAATTCTAAGTAAAAATAATTACACACATTTTGGTTGAACTCATGCTTATTTAGATAACATGATGATAGGAGCGTTTAACTCTGGAGAAAATTGAGAAGAAGTTTTTACTAAAAAAATCGGTGACAACAAAATTTTTGATTTTAATGAATCAAACTATAAATTAGCTATTGATTCATTTATAAATTTTGTTAAAAATTCTACCGGAAACGATATAAAAAACAATAAGAAGCATTACTTTAGTGGCGATGGTCTTGAATTATTAAATCATCTAATTGAACCAAAAACTTCTAGATCTGATGCATCAATAATGTACAACGGTGATGCATTGGATGCATTCTATTCTGAAGATAATTTTCAATCGGTTGAAAGTGGTTTAATAAGGTTTATTAGACCTAAAAACACATATATGCTAATGGATAATTGAATAATTTCAAAAAGCCTTTCAAAAAAAGAAACAACAACTTTTTTAAAAGCCATAAGAGATCATGTTATAAATATTGATAAACACAAAAATAATATTCAGAATATTGAAAAAATATTTTTTAATCAATTTAAAAACAACATGACAAAACAATTAAAACAATCTGCTGAAGCCTCATTTTTTGAAACTATACATAATATTGATAAAACCTTAAAAAATAAGTTAATTCAAGAATTTTTAAAAAATAAAGAATTTTTCAATATAAACGAAAAATCAACTAGTTTTGAAGAATGAAATAGTAAAAAATTTAGAAAATTTTTAAAAGATCCGACTATTCAAGAAAATTATGAGTGGTTTTTAATTTGAAGGAACAATTATCAAGACCATAATGAAGCTTTCTTATTTGACGATCTCATGTCTGATGCTTTTGCAAATAGTGAAATAGGAAGCATCTCTAATTTTGATTATGTGTCTTATACTCCGACCGAAAAAATAATTTATGACTTTATTAAAAAATGATATTTTGGTTCAAATAAAGTGGCAATATCAATATTCGATCAACCCGAATCAAATAACAATTACCAAGTTTTCTCATATCCAATTATTGACAATAATTTAAGAACAAAAATAATTACTTATTATTATGAAAAAACCAAGTCCTAAAATTAAAAGATCTATTCATAAATGTTTAATAAGATTTACAAAATTTAAATAATATATAATTTTTAGTATAAATTAAAAATTGAAAGGAAAAAACGTCATGAACATAAAGCAATTATATAAGCAAATAAGCCAGTTACAAGAAAATGTTGAACTTACAATTGAAGGTTGAATTATTTCAAACCGTGGAAACGATAAAATTCGTTTTATTACAATTAATGATGGTAGTACAATTGCTAACATGCAATTAGTTGTTAAGGGGGATCTAGCATCAAAGAAAGAAATCTCTGAAATTGCCATGGGTTCAGCAGTTAGTGTAATAGGTAAATTACACTTAACGCCAGAAGCAATGCAACCATTTGAACTAAGTGTCTCAAAATTTTTATACATAAACAATCCACAAGAAGATTTTCCAATCCAAAAGAAAGAAACATCTCTTGATTTTTTAAGAGAAATTCCCCAATTAAGACATAGAACAAGTTTATTTAGAGCAATTATGTTAATCAGAAACTCTCTTATGTTTGAAATTCATAAATATTTCCAATCTAAAGGCTTTTTGAATCTTTCATCTCCAATAATCACATCAAACGATGGTGAAGGAGCAGGAGAAACTCTACTTGTTGACGATGAAAGCAAAGATTATTTTTTCAAACAAAAAGCATTTCTAGGGGTAACCGGACAATTGCACGCCGAATCATATGCTGAAGGATTTAAAAAAGTTTATACATTCGGGCCAACATTTAGAGCCGAAAATTCTCACACTTCGCGTCATCTAGCAGAATTTTGAATGATGGAACCAGAAGTGGCCTTTTGTGATTTAAAAGGTATCATTGAAATTACAGACGATATGTTAAAGAATGTTATTGCAAATACATTAAAAAACTATCCCGAAGAAATGAAATATTTGGATTCATTACAAAACAATAAATTAATCGAATCATTAAACAACTTTATCAATAAGAAATTAACAATAATTGAATATAAAGATGTTGTTGAAAATCTTAAGAACTATGTAGATAAATTTGAAGAAAAAGATATCTTTTTCGGAATGGACTTAGCTTCAGAACACGAAAAATTCCTTGCCGAAAACATCATAAAAGGCCCTGTTGCAGTTATTAATTATCCAAAAGACATAAAGGCTTTTTATATGTATCAAAATGATGATGGCAAAACAGTCGCTGCCTTTGACTTATTAGTACCAGGAATTGGCGAATTAGTAGGGGGAAGTCAACGTGAAAATAGATATCAAAAGCTTGTTGACAGAATCAATGAACTAAAAATCACTCAAGAAACATTACAATGATACTTAGACTTAAGAAGATTTGGTTACGCCCCAAGTAGTGGTTTTGGTTTGGGCTTTGAAAGACTTGTAATGTATGTTACTGGCACAAGCAACATAAGAGACGTTATTCCTTTTCCTCGTGTTGCTGGCTCAATTAAAATGTAGGTTTCTATGCAAGAAAATAATTTAACAATATTAATTCCTATATATCATCCAACTCTAACAATTAATGAGATATTAAAAAATTTGTATAAACAAAAACAACAAAATTTTAATTTAGTAATCGCTATAGATAAGCCTTCTGAATCAGAACTTTATGAATTAGAAAAAGCCAAAACCTTTTTCTCAAATAGACTTCAAATAATAATCAATACAAATCACCAACACATTGATTCAATAATTAGTAGTTGCTTAAACATTACAAAAACAAAATACACATTTATAATGTATTCATATATTGAAATTGAGTATGATTTTATTTCAAAAATAAATAAACTTTTATTAGAATTTAAAGATGTTGATATTATAAGTTTTGACGCATATACAAAGGGTATATCTTGAATGAATTTCAACAAATATCCTAAACAAAATAAAGAAATAAATATTCATATTGAAGAAGAAAATGTTGCTAAAATAATTCCTTTTATGTACAATTTTATTGCAAAAACTTCTTTACTTATTGGTGCAACAAATCAACACAATAATAAACACCTTAGCGAGCAATATTCACCCAATATACTTTTTAAGACTTTAATTAATGCAAACAAAATTCTTTTAACAAAAGATATTCAAGTAATTGATTGAAATTATGATGTTCTATTGTGAAGCGTTAAATCTTTATTAGAATCTTGAAAAGACATAGAGTCTGAGTATTCTATGCATGAATTTACAACTCCTTTTTCAGAAAGTTATTATTATCTTGCAAAATTTTTAAATATAGCATATTGTTTCGCAGGTTTTTTAGGCCAATGTCAATTTAAATCAAACACAAAAAACTATCTAACCTTAAAAAACCTTAAAAAACATTTAGAATCATCGATAAGTGAAAACATTGAAGAATGAAAAAATAGCAAAATTATCAATGATTTTTTAGAAAAAAACCGTATTCAACAACTTTTTGAATTAATACCAAATAGTAAAAAATGATCACTTATTTTTAAAAAAATAATATGATAAAAATAAATATTAAAGCTAATTTTTGAATAAGACTTGCTGCTACTTTGATAGATTTCTTGGTATTCATTGGTTTTTTAATTGGTTCTTCGTTTGCAATATTTAACTACAAGGAAGCAAAATTTTATAATTATTGATCTTATTATCTTTGAATAATAATGATAATTTTAGTCCAAAATATAATTTGAATAATCATTCCAATTTTTACCAAAGGTTATACTCTGGGTTTGTTTATTTGCAGACTAAAAATAATCAAACAGCAAAACAATAAATCGAAACTTAGCAAAGTTATTTTTGATAGGCAAAGGTTATTTAGTTTTATATGAATGATAATTTTTGCTTTATTTATGATTTTTATATCTCCCCAAACTTTTGAACAAGCAGCAAATCTTTCTCAAACAAAACCTCTAGACCAACTTCAAAAGGCTTTTCTTTCAATTCCTAGTGTGCTTTCGGCATTAGCCACATTTTTAGAATTCTTATTTATTTTAAGCAATGCTAAGGGAGATAGAATTGGTTTAAATGATAAATTTTCCGGATCCTATACAGTTTGAGCTAAAAAATATGAAGATATTGAAGAAAACAATATCGATAATTTGATTATAAAACCCATTGTTAGAGAATTACCAAAAATTGATTACAAATAGCAAAAAACAAATATTTCAATTTTGGTTAATTATAATTGAAATATGCACACCACAGAGGGGGTGTTGCTTTTTTATATTGCACTTTTGTGATATAATTTTATAATAAAATTTGCACATAAAAGGGGAATAAAATGATTGATTTAAAAGATTTAAATGAACAGCAAAAAAGCGCCGTTATATATAACGAAGGACCTTTAAGAATTATTGCTGGCGCTGGTTCAGGAAAAACTAGAGTTTTAACATATAAGATTGCTTATTTAATCCAAAAATTAGGTGTTCTACCTAATCGTATTTTGGCACTAACTTTTTCAAATAAGGCTGCAAACGAAATGAAACAAAGGGTATTTAATCTACTTGCAGAAGATAACCCTAGCAATCTTTCTCCTTATATTTCAACATTCCATGCAATGTGTGCAAAAATATTGAGAAAAGAAATATATAACTTTGGATATGAAAACGATTTTCAAATTCTTGATGAATTAGACCAAAAAGAAATATTAAAAATTGTTTATTCAGAACTTGATATTTCGCCAACAGAATTTACATTCTCTTCAATAATTTCATTTATTCAAAACAAAAAGAATTCAATCGAATATTTAAACAACTCAATTAACGATAATAACGAACATTTTAATGAAGACTCAGATTCACAAGAAACACAAGATGAAAAAATCAAGGCAATAAAAGAAAAAATTTACGAACGCTACCAATTACACCTTGAAAGATCTCACTCCCTTGATTTTGATGATTTGTTGGTGTTTGTATACAAATTATTTTATGAACCAAAATTCAGTTTTGTAGCCGAAAGATGAAAAAAGAGATTTGATTATATTTTAATAGATGAATTTCAAGATACTAGCATTTTGCAATATAGAATAATGCAAAAACTTGCAAATGAACATTTAACCATTGTTGGTGATCCAGATCAAACAATTTATTCTTGAAGAAATGCTGACATAAACATTATTATGAATTTTGATAAGGATTACCCAAATTCAGTTACAGTAAAACTTGAAGAAAATTACAGATCAACCAAAACTATCTTAAGAGCAGCAAACAACTTAATTTCGCATAATAAACTTAGATTAGAAAAGAAATTATTCACTGAAAACGAAGAAGGCGAAGATATTGAGTTTTATTGTGGTTTTAATGACGAAGCAGAAGCTAGATGAATAGCAACAAAAATTTCTGAATTAAAAAGGAATCGAGTTCAATTAAAAAATATTGCTATTTTATATAGAACCAATAGTTGTTCAAGAGCAATTGAAGAAGCCTTAATCAAAGAAAATACAATATATAAATTATTTGGCTCTATTAAATTTTATCAAAGAGAAGAAGTAAAAGATGCCCTAGCATATTTAAGAGTTATTCATGATGGAAGCGAAATTTCGTTATTAAGAATAATTAATAAACCTTCTCGTAAAATAGGGAATGTTACAATTGAAAAACTTCTTTCTGCTTCTAAAAAATACAACTTAGGACTTTTTGAATATCTAGAAACTCACTTTAACCAAATTCAAAAAGAATTACATATTTCCGGCGATACATTAAAGAATTTAGCAAACTTTATCAATGAAATCAGATGAGCTAGAAGAGCAATTCAAACAAATGCAATTCACTTAACCATAAAAGAATTAATCGTTAATAAAATAAAATACTTTGACGAAATAAAAAATTCAGAAGAAGAATATGAAAATAAAATCGACAACTTCAATAGTTTAATTGAAGCCATTGCTGAATGAGAAAAGAAAAACCCACAAGGTTCTATTGATGAATATTTGCAAGAAATAACTCTAATAACAGATAGAGATGTTGAAGATGATGCCGCAAGTTTCGTTTCACTAATGACTGTTCACAACTCAAAAGGGCTTGAATTTGATTATGTATTTGTTGCTTGCTTAGCAGAAAATATCTTTCCATTAAAGAGGGCCATATCGATTTCTCCACAAGACGATTTTACTTTTTTAAATTCAAAAAATTTCAAAGAAAACATAGAAGGATTAGAGGAAGAAAGACGTTTGGCTTATGTAGCAATGACTCGTGCTAAAAAAAGATTAATGCTTTCTTTCTCGGTTGGTAGAAATGGTATAAATAGAAAAAGTAGATTCTTGTCTGAAGCAGGAATAAACGAAATTAGAACAATAAAGATTGCCAACAACTTCTCAATTGCAGCAGAGCAAGAAACAAGAAATAACGATCTTATAGTTGGCGATTATATTATTCACAACACATATGGAAAAGGGTTGATTATAGATATGGTTGACAACGTTATTGAAGTTAAATTTGAAAATGATAAAAAGATTCGCAAATTATCAAAAACCCACCATTCAATTAAAAAATACGAAGATGATGAAAGCCTAAATAGTTAAAATGTATAAATACATAGCAATATGAATCTTTCTAATAATTTTATTCTTGGCCGTATCAACCGTATTAACGATATATTACGTTATTATTAGACAAAAGAAGATAAATGCACAAACAGGATTTTTTGTATTTAAAATAGACAGTACAAAAAACAGAATAAAAATCGAAGGCGATATAAATTCAATTAACAAAATACCTAAATATTTATGAAAGATTTTAAGTATAGATGGTCAATGGGCAAAACTAAATAACTTCTTAAATATTTTTGATTCAGGTGATAAGCGCCTATTACTAGATAATATAAAGAAAAAAAACTTCTTTAAATTAAAAACAAAGATAGCAGAAGAATCATGATCTAACTATTTTTCAAAAGTAGATATTGAGTTTAACAATGTTGTAAATGATGAAATTATTGGAACATTTACATGAAGAGACATTGACATTAAAATGGATAATGTTTTCAATAAAGAAATTCATGATTTGTCTTATATTCCAACCATCGAAAATTATGAAGGCTTGTTTTTCTTTTTAAACAAGAAACACATTACTGATATAAATGCTTTTTTAGATCTGTTTAAAGATTTAGGAAAACAACACAATATTAATGGCTTAGAAGTTGTATATGAATGAAATGTTCTTTGCATTTTGTTTAATCATGATAAATTAGGTATTGAGAAAGCAAAATTTCTTGCACAAAAATTCATCGATATTGCAAAGCTATACAACAAACTTTATTTTAAACTTTGTTGATTTGATTCGTATATAATGAATAAAAAAACATTGGATAACGTATTTACATTCTTTGATTATTTAAAATTAGAAACATTTGAAAAAAATGCATATAACTTTCAAAAAATTCCAAGCAATATTTGAACCGAGCAAAACTTTCAAAAATTTTGTTTAACATACGAAAACATTATTTATAATCTAAGAAATAATTTCTCTATTTCACTAAAAAATGTTTCGCTAAAAAATGCAATTGGCGATAAAACCGAACTTAGAAGGTTTGGACTTCCTAAGATTTTTAATTCATTAAAATTAAACAAAGATTATGACTATTACCTAGAATTTTTAGACATAACTAGATTATTTTATAATGTCTTTTATGAAAAAATTGGTGAACTACATCCTAAAAATGCAATATTAAAAATTAGTGATTCAATATTTGAAACTTTAGATATAAACGTTATTAAAAAAATCGGGGTTGAATTTCCAACATTTATTCAATCAATAAAATTATTATCAGCAGAAAGAATAAAAGACATATCCCAAAAAATTGTTAAACTACAAGGAACAAAACTCAATTTTTGTCTCGAAATTAAGGATATTGATTCAACAATAATTTCTCTGGTATCCCCTTGAGTTAAATTCATTTGAATAGATGAAGAACTAACCTCTAGATTAACCGAACCTAACATTGTTTTATATCTAAATACCCTATTAGATAATGTAAAAAATCTAGGTATTAAAGTTATTTTTGAAAAATTAGACTACAAAACATACAGGAAATTATTTTATAAACACACATCGATTATTTTATATACGACCAATTAGCGTTTTAATATAAAATTATTAAAAAATTCAAAACAAAAAAGGACAAAACATGAACAAAAAACTTATTCCAATAGATTTCTTTGATGCAGTAAATAAAAAGTGGGTAGATAGCCATAAAATCCCTGATTCTAAAACCTCTATAGGTTGCTTTGCGCAATTAGATAAAAATATTACTACATTAACTAAAAAAATATTAAATACATGAAGTGAGGATGCCTCAACAATTCCAAACGACCCTATTATCAATGAACTAGTAAAATTTTACAGCTTAACCAAAAACTGAAAATTACGTTATCAAGCACAATTAAAACCAATTAACAAGTTGCTTTCAAAAATTAACTCTTTAAATTCTTGAAAAGATGTTGAAAACAATTTTTTGGAATTGAAATTATCTAGTTTAAACACCCCTTTAATTTTTTCAGTTTCAACAGATTTTAAAAACAGCGATGTTCAAACTCTTTATTTAGATGTAAACTCTACAATTCTACCAGACAAAAGTTTTTATACCGATCCCACCAAAAAAGAAAAATTTATTAATGTTTGAAAAAATATGGTTTTAAAACTTTTAAATAAGATCGACAAAAACGAATCTAAAAATAATTTAATCATTGAAAATGCTTTAAAATGAGATGAATTAACTTCTTCATTTTTAAGAGATTCAGAATTCTATTTTGATATGGTTAAAATTTGCAACACTATTTCAACAAAAGAATTAGCCCAAAACATTAAAACAATAAACATAGAAAATATATTAAGACAATTAATTAAATGTTTCCCTGATGAAATAAATGCTATTGATCCAAATTCTATAAACATTGCAAAGTCTTTAACCAATGAAGAAAATTTTGAATATTATAAAGCAAGTTTAATTATTGATGCAATATTAACCTATGCACCTTATTTTGATTATTCAACAATTCAAATTGCTTCTGAATTTAATTTAGCAATAAACGGAAACAAAAAAATTGAATCAAAAGAAAAATATCAAATTAAACAAGCAAAACGTTTTTTTGATATTCCTTTTGGAACTTACTATGGTAAAACCTATTTTGGATTAAAAAACAAGCAAACAGTTGAACACATGATAAAAAGTATGATAGATGTTTATAAAGAACAACTAACAAAAAACTCTTGGTTAAGTAAGCAAACAAAAGAAAAAGCAATAACAAAACTTAACTCAATAGGTGTTTACGTTGGCTTTCCTGAAAAAATCCAAGATCACTATAAATATTTAATAGTTGAAGAATTTGATGGATACAATGATTTATTTGAAAATGTTTTAAAATTTAACAGAATTTTAGCAGAACATATCTTATCCGAATATGGAAAAACAGAAGACAAGAACCTTTGACATATGTCACCTGCTATGGTTAATGCTTATTATAATCCTTCAAAAAATGTAATAGTATTCCCTGCTGCAATCTTGCAAAAACCTTTCTTTAGTGCAGATCAATCCTCTTCATCAAATTTCGGCGGAATTGGTGCTGTTATTGCCCATGAAATATCACACGGATTTGACAATAACGGAGCAAATTTCGACGAAAAAGGAAATATGATTAATTGATGAACAGAAGAAGATAAAAAAGCTTTTGAAATCAAGACAAAAGAAATGATTAATTTATTTGAAGGTAGAGAAACATCTGTTGGTAAATGTAATGGAACATTAACCGTTTCAGAAAACATAGCAGATGCTGGTGGATTATCGTGTGCTTTAGCAGCAGCAAAATTAGAAAAAGATTATAACCCTAAAGATTTTTATATAAATTTTGCAACAATTTGAAGAACAAAATACCGTCCAGAATTACAATCATTATTGTTAACTATTGATTGTCACGCCCCTGCCAAATTAAGAACAAATGTTCAAATACAAAACTCTGATGATTTTTATACAACTTTCAACGTCAAAAAAGGCGACCCAATGTATTTGCCACCTGAAAAAAGAGTAAAAATTTGATAATTCTAAACACAAAAAAAGCAACCGCACGGTTGCTTTTTTAATTAACACAAATCTTCTTTCTTTATTCCTAAAAGAACGTACAACATCACTCGTTTAATTCTACTATTTGTATATCTTTTTGAAGTACATAAATCAACAAATTCATTATAATTTTTTGCTATTTCAATATTCTTTTTAAAAAGATTTTCCATTCCCTCGGATACTAATGCAATATTTGCTAGTTCTTCTTTAGAATAATTTTGTACCCTTTGTTTAAATTCAATATATTTATCTTCAATTTTTGAAAAATTTTTAGGAATGTTTATTGGTGTGAACATTGAAACATCTTCGCCTTTTTCTAACATTTTTCGTATCATTGTTGCTGAAGCATAATTATTATTTGCTATTAATGAATTGTGTGGAGCTGTTCTTTTTATGCAAAACGGTTTTACTGACAAATTATTAAAAACTATGTATTTGGTATATTCAAACCCCAAAATATCTTGTGGTATATTTTCAACACCTATTAATTTTTCTAGCGACAAAAAACAACTCAAAACATATGATTTTCCTGTTTTTAAATTCATTCTTACATCTTTGTTATATTGATCAAAATTTTTCTCTATTACGTTTGCTGCGTTTAAATATTTTTTTATATCATTGGTGTCAGAAACCCCAAAAACTAAATAATCAATACCTTCTTTTATTAACAAATCTATTGATTTTTTAGCAAAAATATGAGCCGCTTGTGTAGATGCAAAAAAATCTAATTCAACAACTTTTGTTGCTCCATATTCAAGCGCAATGTTTTTTCTAACTTCAAAAGGTAAACACGCTATTTCACCACGTTGAACATAATCGCAAGATAATGCAACAATTATTTCAGGATCTTTGAAGATTTCTTTAATTTTATTAATCAAATAAATATGCCCATTGTGAAATGGGTTAAATTCAGCTATTATTCCTATTTTCATTTATTTTTTCTTTAATTCATCAAGGTTAATATCGCAAAAATCTTCAATAGAACTATCTTCTCAATAATTTAATTCCACAGTTTCTTGGATTTCAATATTTTTTTGATCGCCTTCACAATTTTTGTTTGAATCAAAATCTTCAAACATCACAATATCGTTTTGTTCTTTTTCTTTTTTTATTTGTTTTTTCTTGCTTGTTTTCTTTTTATTAAACATATTTAAATTATATCATTGCAAAAAATTACTTTTGTTTTTTTAATTGTGTGATTAAAATGTTAATAAATTTTTTCGAAATTTCTTTTTAATTTTAGTTGTTTTTTAAATTGTTAACATTATAAACATAGCTTATTATATTTATTATTTTAACAAAGCAAACCAATAACACCTAACATTAAACAAAAACATTTTTATATATTATTTAATATATAATTAAAATATGAAAAATAATATTCAAGATACAATAGTTGCTATTTCTTCAGGTAATGTAAATCAAGCAATATCTATTATCAGATTATCTGGTGAAGATTCGATTGAAATTGTTAAAAAAGTTTTTAGTGGTAAAGTCGGAAAAGATAAAACAATTACTTACGGAAATATCATTGATAATATAACAGGGAAAATTGTTGATGAGGTATTAGTTAATTGATTTATTGGTAATAAAAATTATACCGGAGAAAATACCGTTGAAATAAATGCTCATGGTGGAATTGTTGTAACAAACAAAATACTTAATCTATTAATTGCTAATGGTGCAAGGTTGGCAAATAGAGGAGAATTTACAAGGCGAGCATTTTTAAACGGAAAAATTTCATTAGATAAAGCCGAAGCGATAAACAGCTTAATTCATGCAAAAACAAATAAACAAGCAGAAATTGCTATTTCTCAATTTAATTCTAAGGACAATTTAATTATTGAAAATTTAATCAACGAACTACTTACTATAATTTCATTAATCGAAATTAATATTGATTATTCTGATTATAACGATATAAAAGAAATGGATGCTAACACCTTAACAAGTCAATTATCAAAATTTAATAGAAAATTAGATTCTATAATTAATAAATCAGAAAATGCTTGCGATATTTATAAAGGAATCAATGTTGCAATAGTAGGAAGACCCAACACTGGTAAATCATCACTTTTAAATTCTTTAATTGGTTCACAAAAAGCAATAGTAACAAATATACCCGGAACAACAAGAGACGTGGTAGAAGGTGAATTTCAGATTAATGGTTTTTTATTTAATTTAATTGATACAGCAGGTATAAGACAAACAAAAGATCTTGTTGAATCAATAGGTATCGAAAGATCTAAAAACGCAATAAAAGAAGCCAAAATTGTAATTCATATGCACGAACCATTTATTAAAGAAAATGAAGAAGATCAAATAATTAAAGAACTCTCAAAAGGTAAGGTATATATCCCAGTAATGAATAAGCAAGATCTTTTAAATAAAGATCAATATCCTAAGAACATGGTTTTAATCTCAAGCAAAGAAAATAGTCTATGAGAATTAAAAAATGCGTTAGTAAAAAATTACTTGAATATTGATTTAGATGACCCTCAAATAATTTTTAATAATAGAAAACTTTCGTTATTGAAGCAAGCGAAGATTTCTATCAATACTGCACTAGAATCACTAAGTCAAGGTTTAGGTCCTGAGGTGGTTATTTTAGATATAAACGAAGCTTGAGGGTCATTGAGCGAAATACTAAATAAAAAGCACGATAATGAAGCTCTACTAGATAATATATTTAGCAAATTTTGTTTAGGAAAATAATATGTATAAAATAAACGACAAAATAGTTGTGAAAGCAACAGAACTAAGTTATGAAGGCTATGGGGTTGTAAGAAATGATAAACAAACACTTTTTGTTGAAAATCTCTTACCTGATGAAGAAGCAGAAGTAATAATTTATAAATCAAATTCAAAAATTGCTTATGGACGCACAATTAATTTGATAAAAAAATCAAGTAAAAGAGTTGAAGTTGTTGAGAAAGAATTATTTGAAAGTGGTGCTGCGCCATTGATGGTTTTAAGTTACGAAGATCAATTAAAATTCAAACAAGAAATTGTTAATAATTTAATAAAAAGAAATATTGGATTTAAAAACATTGAACAAATTCAACCCAGTCCAAAAATATTAAATTATAGAAATAAAATAACATTGCAAGTAAATTATAAAAACAACAATATTTATATTGGTTTCTATAAAAAAAATAGCAACAAACTAGTTGTTCAAAATTCGCTTCCTCTTTGTCATGATGAACTTGATAAATATTACAAAGAACTTGTTGGCACCTTAAAACAACAAATTTTATTAAAAGATAATTGAATTAAAGAGCTTAAACTTAGACAAATTACATTGAGGCATAGTAATTGAAGCCATAATATTGAAGTTATTTTTATTATTGAAAATCCAAATAAAAAAGGTTTTGATTTATTGAAAAATAATTTGATTTTCTTATTGAAACCCAATGTAATTTCAATACAATGCCAGCACTCTCAAGAATCTAAAGATATATTCACTTCTTTATACAAATCAATGCCACAACAATTTAATATAAACAATAAAATATTCAATGTGAACAATGATTCTTTTTTTCAAGTAAATGAAGAACAAACTAGTTTAATATATAATCAAATTAAAAAATTCATAGATCAAGAAAATTCGGCAAAAATAATTGATGTTTATTCAGGTGTTGGTAGCATTGGAATATCTGTTTGTGGCAATGACTCTCAACTTGAATTAATTGAAATAAATAAATCAGCATGTGAGTGTGCAAAAAATAACATAAACCAAAATTTGATTAATCCAAAATTAGTGGATATAACCAATAGTTCAGCGCAAGACTTTTTTAATAAAAAAGAAGCAGAATATTTTGAAAAATCAATAGTTATATTTGATCCACCAAGAACGGGCTTAACAAATAAAGAAATTGACATGTTAGAAAAATGCAATAGTGTAATTTATATGAGTTGCAATCCTCATACAATGATTAGGGATTTAAAATATTTTGAAGAAAAAGGGTATAGCATTGAAAGGCTAATACCTTATGATATGTTTCCTCAAACTTATCATATTGAAATGTTGTCACTTTTAAAAAAATAAAAATTAGGTTTAATTAAATTACCTAATTTTTATTTATAACTTTTTAGCATATTTATACCATTTTCTTGTGCTTCAACGAATATTGAATAGCAATGTCCTCGTGAATGCATCTAATAACGTTGCTCCTGTCATTAAAAACATGAAGGTGGCAGGACTGAAATAATGATGTCCTAATTGAATATACACAACAAGATATACGGCGCTCAATTGAACACCTAGCATAGTTATTAAAGAAACTAAATTTGGCATTCAAACATCGTTGGCACCTCATAAAGCCCTTAGGGTTGTAAGGTTTCCTATATCTAGAACAGCTCTTAGCATCATAATTCCCATAACACCATACCCAACAGAATTAATTATTTGAATATCTATTGAATAAAGTCTTAGCAAAGGCCAGGTTAATGAAACTCCTAATATCGAAAATAATGACTGTGCATAGAAGCCAAATTTTAATGCACTATTTCCAAGTTCATGAGCAGCTTCAAAATTTTGTTTCCCTATTTCGTTTGCAACAATTGCGCTTGATACATAACCCAAGGCGATCATTACTGCATTAAATATGTCATAAGAACTATTTACAGCTCTTCACAAACCAATGAATTGTTCGTTCTTTCCTTCAAATCCCATCGGAATTGCTCTTGCAAGAAGATATTGTCCTATGAATCAAATTCCTGTTTCAAAAGCAATAGGAAGACCTAACTTAAGAATGTTTTTAACATATTTTGTGTTTCATGTTTCTTTAATTGAAATTTTATTGTTTTTGGTTATAAAGTGATAGGAAATTGAATAATCAATTGTTAAATGAAATATTGCCCCCAAAATTGGTTCAAGTGCAACCATTATAAAAAGATCTTCGTTTGAAATCTTTCAAGGTAAAAACATAATTAAATAAGTTCAACTTGCACGTACAAATATACTGGTAATTAATCCAAATATTGAAAATTTATTTTTTTCAACAGATTGTAAACTGGCTGTAAAAATAACAGCTATTGAATATATGAATATGTCGCAAGTGGTTATTCTTAAGAACTTTATAGCCATGGTTGTTTCAGAGGTTACTGAAACCTTAAAAGGATTTGTATTAACTAGGGTAATTCCGTTTATAGTTCCACCGTATAGCACCTTATTAAATTGATTCATTTTAGCTAAATCATTTATAGTGATGTTGTGATTTTTCAATAATTCAATGTTTGATTTAAAAAGATCTACACCTTCCTTGTTTTTTCAACCAAAAAGAGGAATGTTTTTTGTTCCAGAAGCTTGTAAAACTATTGGCGCAAGTCCAAACATAATAAAATAAACACAAGTATTTATTATTAATGATACATAAACGGCAGACCACAGAGCTTTTGGTATGTCTCTTATTTTGCTTTGTCCAAAAAGATTTGAGCACAAGACTAATGTTCCGCTTTGGTAAATAATCGGAATAAAGGTTATCATTTTGTAAACAGTTAATATTTTTGCAATCATATAAAAATAAAAACCGTTGTATGAACCATCGGCGTGATAGACTTTTATATAATGCGAAGAAGCAAGAGAAGAAACTACCGATACCAAAGCAACAAAAAGTGTTTGAAAAAAGATTGGTAAAGTCAATTTGAAAATATACTTAAAGTGCTTAACTTTAAATAATTCATTTGATTTTTCAATAAAAATATTTAAACGATTTGTATTTCTTTGTTTACTGTTTTCTTTCTTTCTCATAACTCGTTAATTCTAACATATAAATTAAAATCCATTTAAAAAACTTTATTATAATGTAAAATTTATATAAATAACGCTAAAATTTGCAAGGAGATTTACATGGGATTTTGATCAAACTTAAAAGATAAATTATTTGGTACCAAAGAAGAAAGAATAGCCAAAAAACAAGCAAAAATTGAAGCAAAAGAACAAAGACAATTAGAAAAAGAATTAATTAAAAAAAATAAGTTAAATACTTATATTGCAGGTCTTTCAAAATCAAACTCTTCATTTAATGAGCATATAAAAGAATTGCAAAACAAGCATAATAAAATTGACGAAGAATTTTTTGAAGATTTAGAAGAAATGCTGATTATGTCTGATATTTCGATTAAGCTAGTGCAAATAATTATTAACGAATGTAAAAAAGAAGTTAGAAATGAAAATATAACTGATCCAAAATTAATAAACGAGATAATTGCTGACAAACTGTTTACAATTTATACATCAAATTCTGTTGTCGATACAACACTTAATATTAAAGATGATAGGTTAAATGTAATATTGGTTGTGGGCGTCAATGGTTCAGGAAAAACTACATCAATATCAAAAATTGCTAAGAAATTAATTGATGAAGGTAAAAAAGTATTGATTGCTGCAGGTGATACATTTAGAGCAGCGGCAGTTGAACAATTAGAGATTTGAGCAAAAAGAGTTGGGGCTGATATTGTAACACCAAACGAAAATGAAGTGGATCCAGCGGCTGTTGTTTATAGAGCAATCGATATAGCAAAATCAAAAAAATACGATATATTGATTGTAGATACTGCGGGAAGGTTGCAAAATAAAGTAAATTTAATGAACGAACTTGCCAAAATTAATAGGGTTTTAGCCTCTAAAATTCCTGATGCGCCTCATGAAAGTTTATTAGTTTTAGATGCAACAACCGGACAAAATGGTGTAATTCAGGCTAGGGTATTCAAAGAATCTACACCGCTGACCGGGATCATTCTAACAAAAATGGATGGAACCTCAAAAGGTGGAATTGTTTTAACAATCAAAGATGAGATTGGTTTGTTTGTAAAATACATTGGTTTAGGTGAAAAAGTTGAAGATTTAGCGGAATTTGATTTAGATTCTTATATCTATGGTTTAACAAAAGGTATTAACGAATAATGGAAACAATTGAAGAACGAGATTACTATGTAAAGTTGTTTGAAAAATATGGTGATTTATTACCACAATCTCAAAAACAAGCTCTACAATTACTTCTATATGAAGATTTGTCATATGGAGAATTAGCCCAAGAATTAGCGATGTCTAGACCAGGGGCTTACGACGCGGTTAAAAAAGCAAAATTACACTTAAAACAAATTGATGAAAAGTTAAAACAAAGTTAAAAAATTTCTATTTTTTTGTTATTAAACAAATAATTTATGAAAATAATTAAATTTAATATATAAAATATATATAAATTATTAACCAATAAAGAGGTAAGAATGTCTTATAAAAGAGTATTGATTAAACTTTCGGGTGAGGGTTTAGCAAATAAAGGTAAACACCTTGCAATTGATTATGAATTAGTTGAAAAATTTGCAAACCAATTAAAAACAATAATTAGTAGAAATATTGAAGTTGCGATTGTTGTCGGTGGCGGTAATTTTTGAAGAGGAACAAGTGCCGAAAAAAATGGTATGCAAAGGGTTAGAGCAGATTATATTGGTATGTTAGCTACAACAATGAACGCGTTAGCTTTGCAATCAGGATTTGAGAGATTGAATTTGCATTGTAGAGTATTATCGAGCCTAACAATGGACCCAAAGGTTTGTGAAGTTTATATAAATGAAAAAGCTAAAAAATACCTAAGAGATGGAGAAGTTGTAATTTTTGCAGGAGGAACTGGACGTCCATTTTTTACAACCGATACAGCCTCAACATTATTCGCTAGTGAAATTGATGCGGATGCAATATTGATGGGAAAAAACAACGTTGATGGGGTGTATGATAGCGATCCAAAATTCAATAAAGACGCCAAAAAATTTCCAATCATTACATATGATGAATTACTAGAAAAGGAATTGAAAGTTATTGATCAAACCGCAGCATCAATGGCAAAGGAAAATAATATTGATATTTTAATATTTGATATTAATGAAGAAGATTCTTTAATAAGGGCAATAGAAAATAAAATACCTAACACAATAATAACTAGAGAAAGGGAAAAATAATGGAATTAGACAGTTATCTTTTAGAACTAGATGAAGAAATCAGCAAATCAATTGTTAACTATGAAAATCAATTAATGAAAGTTGTTATAGGTAGGGCCAATCCTGTTTTAATCAGTAAAATTAAATTAGAATATTATGGTTCACCAATTTCGGTAGAAGAAATAGCATCTATAACCACATCAGGACCATTACAATTAATTGTTAAGCCATATGATATGAGTGTGCTAAAAAATATTGAAAAAGCAATCATGGATAACAAGATAAACGTTTCAATAATAAATGAAGGACACCAATTGAGATTGCAATATCCTCAAATGACCACTGAAAAAAGAGTAGAAATGACAAAGCAACTTGCTTCAATAACCGAACAAGCAAGAATAGGCGTAAGACAAGCAAGACAAGATATCAATAAAAAAATAAAAGCAGATAGCGACCTTTCAGAAGACATTCAAAAACATTATCTAGATAATATTCAAAAAGAAGTTGATAAATCAATAGAAAAAATCAATGAAATGTCAAAAACAAAAGAAAAAGAACTTTTAACAATATAAAATGCTTCGGCATTTTTATTTTTAATTAATCTTTTATTTATTTATAATTATTAAATAATGAAAAACCTAAAAATTAGAGCAAGATCTGCAATTATATTATTTAGTATATTGATTCCACTACTTATTTTAACTTATTTTGTTAAAAGTTTAGGGGTGGCAATTTATATGTTTTTTAGCGCGTGGGCTTTGTATGAAGTTATTTCACACAACCAAAACAAAAGATCTGTTAATTTAGTTATATCCTTACTATCAATATTAATTTTAATAATACCAGTTAATTGATTCTTAAAAGATTGAGGAACATTTTGAGAAATGTATAGCAATGGGCATTGAATCTATGGTCTTGATAGAGATACTTTGATTTCAATAATAAAAAACACAATATTTTTTAAATCAAATGCCTGGTCTGGTGCTCCGGGATTAGGTTTAATATTGACATTTTTAATAACAACATTCTTACTTATTGTTGATGTAAAGAGTCAAAAAAATGCTAAGAATTGGTTTAAGAACTATTTCATTACAATATTCGCCGTTATATTTATTCCATCAAGTTTTAAACTAATTTTTATGTACAACGTAATGAGTTTGTATTGACTATTCATATTATTTTTAATTCCTATTGTGTGTGATAGTTTTGCTTATTTAATTGGTAGACTTATAGGAAGGAAAATAATTAAGTTTGGCTTTGCACCTCACATAAGTCCTAAAAAAAGTTGAGAAGGAGCCATTGCCGGATATTTTATAGGGGCCTTGTTTGTCTTTATATTTATGGGAATTAATTTTGGAAATTCCGATTCAATAAAAATATTAATAAATCAAAAGCAAATTATTACGGCCGTTTTTGTTCTTCCTGCAATTTCTATTATTGGCGATTTAGTATTTTCGGGAATAAAAAGAGCTCAAAATATTAAAGATTTTTCTGAATTAATAGCGGGTCATGGAGGAATTATGGACAGATTTGATTCGCTATCGTTTAATACGGTATTTTTAACTTTTATATTAATGATTTAAGTAAAATAAAATAAAAAAATCGTTATTACTTTTTAACGATTTTTTTATTAATATTAATATAATTAATAATAAAACTATTTAATGTAAATGAGGGGATAATTTATGCTTGATAAGACTTTTGTGCAATTATGCAAAGAAATAAAATTTGAATTTGATGAACAACAATTTTATGGTGCAAAAGTTTTGAGTGTTGAATCAAACTTAAATGAATGGAAATTTAAGATTGCCTTTGCAAGCCATATTGACATTGAACAATACAAACAATTTAGAGAAAAATTAGTTAAAAGATTTGGCAAGGTTGAATTAATCTTGGCTATTGAGACAATTATAAGAAATAAACAAATTATTGTTGATTATTTAAATTATCTAATAGAATGTAACCCCAAGAAATATTGGCCTTTTGTGAAATATAATTATTCATGTATAACTACTTCAACAATTGAAATAGATGAAAAAACATTAACACTTTCCTTACTAACAAATGAAATTTATGAAGTTTTTAAAAGTAATAAAGATCTTTTTAAAGAAGCGCTTGCTTCAATAGGATTTGATTTTTATAATGTAGTTTTTAATTTGGGATTGAATAATAAAAAAAGCGTTGCAAGCAAAAAACTAGATGGTTGAATTGAACAATTTAACAATTTAAAAGATAATGAAAATAACTTAAAAGAAAGCTCTTCAAAATCTAATTTTTATTCTAAAAAAGAATCAGCAAGACCAATTGAGATGGATTTAACTACGGCAAACGAAACGTTTGAAGAAATAAAAATAACCACAAAAGGAGAAGTTTTTTTCATTGAAAGCAAAGTGCTAAAAAACAAAAAAATTCTATATACGCTAAAGATTTCAGATTATAAAGAAGCTATTCTTGTTAAAAAATTTTATGATTCCGAAAACGAAATACCTGATATAAAGGTAGGGGACACGATTATTGTTACTGGAAAATTAAGTGATGATAATTTTACAAAATCAAAAGTTTTAATAGCAATGGGCAAGGAATGCTTCATAAAGACAGAACCATTACAAAAACTTATTGAAGATAACGAAGACAATAAAAGAGTTGAATTAGCCGCAAGATCTAACATGAGTGCTCAAGATGGAATTTCAACGCCCACCGACTATATTAATGCTGCTAAATTTTATGGTCATAAGGCTATAGCATTGACCGACCTAGATGATGCGCAAGGCTTTCCCGAATTTTATAATTATGCCAAAAAGCAAAAGGATGTTAAGCCTATATACGGCGCAACATTAAGCACCGTTGATTCAGATAAAAAATTCTTCTACAACTTTAAAAATTTCAACTTAAAAGAAGAAGAATACGTTGTATTTGACCTTGAAACAACAGGATTGAGTCCAAGATTGGATGACATTATTGAATTTGGTGCCACTATTATAAAAAATAATACTATTACTCAAAAAATTCAATTCTTTTTGCAAACGAAACGTGAACTATCAGCCTTTACTGTTGAAAGCAGCCACATTAATAATGAAATGTTAAAGAACGGTTATGCACAAGAAGAAGGCATTAAAAAGATTTATGAAATATTAAAGGATAAAATTGCAGTTGCTCACAATGCAAACTTCGATATAAATTTTTGCAAAGAAAAATTTAGCAAACTTGGCCTTGATAATTCTAAAATTTTTGGCCTTGATACATTGGCCTTATCTTATTTTTTAAATCCCGATGAATTTCGTCATTCTTTGGGTTATGTTGCTAAAAGATTCAATATTATTTATGATACAACTGTTGCCCACCGTGGTGATTATGACGCTAACGTTTTGGCTAAGATTTGAATAAAAATGATTGACAAACTTGCAAAAGATTACAATATCTTGAACTCACAGGATTTAAACAATGTTGATAACCAAAAAATACTTTCCCATAAATTTACATACGAAACAAGAATATTAGTAAAAAATCAAGCCGGCCTTAAAAAATTATTCAAAATTATTTCGCGTTCTTTAACTTCCCAATTTAAAAAAAATCCACAAATTTACTTTGATCAATTGGGTAATGATGAAGATTTGTTTTTTGGATCAGCAACAAATCAATCATATGTTTGAGACCAAGTATTCACTGGAACAAATGAAAATTTGCTAAATTCACTAAAATATTATGACTATATTGAATTACCCCCTATATCATCATTTCAATATATGATTGATAGTAACCACGTTACATTAGATGAATTAAAATTTTTGTATAAAGATTTAATTTCAAAAGCAAAAAGTTTAAACAAAATGTGCGTTGCTGTTTCTGATTGCAGATATATTTATAATTATCAAAGATTAATTCATGAAATTTATATTGAGGCTCCAACTTTGGGGGGTGGGCTTCATTGGCTTAAGAAGAGACCATCTCATCCAGAATTTAGATATTTAACAACAAGAGAGATGTTAAAAGAATTTGCTTTTTTGAATGATAGTTATTTAATTAATGAAATAGTAATTGAAAATCCAAACAAAATTGCTAATGAAATTTCATCTAATATTCAAGTTATTAAAGATAAATTGTATGTTCCAACTTTTGATAACTCACCTCAAAAATTAAGAGAAGTTGTTTATAGGACTTTAAAAGAAAGATACGGGGATAATCCCGATCCCATCATAGTTAAAAGAATTGAAAAGGAATTAAATCCAATTATAAAGTATGGATATTCTGTTATTTATTGAATAAGTCATAAATTAGTTAAAAGAAGTAATGAAGATGGTTATCTAGTAGGCAGTAGAGGAAGCGTTGGTAGCTCAATTGTTGCCAATCTAGCGGGTATTTCAGAAGTAAACCCCCTACCTCCACATTATTTATGCAAAAATTGTAAATATTTTGAATGAAATAGTAATAAAGAAATTTATTCAGGTTGAGATTTGCCAGACAAGCATTGTCCAAATTGCAACTCAATATTGTCAAAAGATGGGCATAATATCCCTTTTGAAACATTCTTGGGATTTGAAGCGGATAAAGTTCCTGATATTGATTTGAATTTTAGTGGTGAATATCAACCAACAATTCACAATTATGTTAAAGAATTGTTTGGAGAAGCGCATACATATAGAGCTGGTACAATAGCAACCGTAGCAGAAAAAACAGCATATGGATTTTGCAAAAAATATGAAGAAGAAAAGAGAGGAAATTATCAACCTTGAAGTCATTTGTTCTTGGACTTTTTAGCATCTAAAACACAAGGAGTAAAGAGAACAACTGGACAACATCCAGGAGGAATTATTATTATTCCTAAAGAATTCGATGTTGAGGATTTTACGCCTGTTAACTATCCTGCTAACGACACAGAAAGTTCTTGAAAAACAACACACTTCGACTTCACCTCTATTCATGATAATGTCTTAAAATTAGATTTGTTGGGTCATGATGATCCTACCGTTATTAGAATGCTTGAAACATTGACAAATACAAACGCAAAGGAAATACCAAAATTTGATGCAGAAGTTATGAAACTTTTTTATACAACTGAATCATTGAAAATTAGTCCTTCAGACATTGGGGGCGAAACAACCGGGGCATATGGGTTGCCTGAATTTGGAACAAACTTTGTAAGAAAAATGTTAAAAAAAGCTCAACCGAGATCGTTTAATGATTTAATTCTTCTTTCGGGGTTGAGTCATGGTACGGATGTTTGAACGGACAATGCCGAAGACCTTGTAGCAAAAGGAAAAAAATTAATAGAATGTGTATGTTGTCGTGATGATATAATGCGTGGGTTGATTGAATGAAATATTGAGCCCTTAACAGCTTTTCAAATTATGGAAAAAGTTAGAAAAGGAAAAGGCTTAACGCCTGAGTGAGAAGAACTCTTAAAAAGTCATAACGTTCCTGAATGATATATAGAGTCGTTACAAAAAATTAAATACATGTTCCCTAAGGCCCATGCTACTGCGTATGTAATTATGGCTTGAAGAATTGCTTGATATAAGTTATATTATCCATTACCTTTTTATGCATCGTTTTATACAAACAGGCCAGATGCTATTGATATTCAAATAATGAGTAGTGGCGCAGCAAGCGTTCACCAAAAACTTATTGAATTAAAAAATCGCCAAAATTCAACTGGCGAAAAATTATCTGTAAAGGAAGAAGCATTAATTCCTGTTTTAGAAATTACTCAAGAATTATACGCAAGGGGATTTAAAATTAAAAATGTTGATTTAAAACTTTCTTTGGCTGATAAATGAATAATAGATGAAAAAGATAAGGCGCTAATTCCTCCATTTAATGTTGTGGATGGTTTAGGAATTACTGTTGCTCAAACAATAGTATCTGCAAGAAAAGAAGGCTTTTTCCTTTCAATTGAAGACCTTGCTTCGAGAACAAAAGTCAATTCAAGAATTATTTCGGAATTAAAAAAAATAGGCGTTTTAGATTGCTTAGATGATACCAATCAAGTAAGTTTGTTTTAAAAATATCTTAAAATAAAAAGAAAATGAAAATTTTAATAAATTAAAAGTGATATAATAAAAGAGCTCCAATTGGAGCATAAAGATCATTGTTTATTTAAATATATAGTTCGTTTATGTCAAATTAACTAAGATTTATTTGTTAATCTTTATATAAAAGTATAAGTTCAATAATTAAAAGTTTTATATGTTTTTCAACCTCCTTCATATTTTTTATCTATATACTTAAAACATAAAAAATGTGCGTATTTTAATTCAAAGAAATCTTATACAAAAAAATTTTTGTGTAGTTGCTTTAATTTGTTTTTGTTGTCTTATTGAATATATTTTAGGTTTTTGTAAATCCTTTCTTAAAAATGTTATTTCGTATTTGTGTGTTTGCAAAACCAAAGGGGTAAATTTATATTTAATAATATTTTTCTTCAAAAGCATCTATACAAAAATAAAGCTGTTTAAAAGTTTATATTTTTTATTAAAAAAATGTGTAGTTATTTCTTAAACAACGTCAGATAATAAGGCGTTGTTTTCATTTGCCTATTTTTTGTTTTTTAAAAAATGCTATATTTGGTAAAATTTTAATTAAATTAAATAATTATTTATATAAGAATGGAGCGTATTATGTCAAATAATGACAAGTGAAAAAATAAGAAAGTTAATTTAAAGAATTATGTTGTAGAATCAGGAAAGCCAAAAAGAGAATTGTCACGTTCATGAAAAATAGCTCTAACGGGTTTATTTTTAATTGTCATTCCTTCTTTTATAATGTTTTTAATTTTAGGCATAGATGGTTGAATTATAAAATCAACAAAAAATTTATCAAGATGAGGTGTTGAGTTTCCAATAGCATTAGCAATTGCTGCAATACAAATTATTATTGTTTTATTGTTAGTTTTTAAATTTAAGGTATTTAACACTGAAGCATTAACCTTTCTAATTCCAATTTCTTTAGCCATTAATTCATTTTTGGTATCTAGTGGTCAAAGACCAGAAGATTGATATATTAGGGTTTTACCCGCTGTAGGTTTGGTATTCCTTGCAATTCCGATAATATTAATAAACAAGGCAGTTGCTAAAAGCCAAGAAAAACAAAGAAAAATTAAATTACTTGAAGAAGACCAAAAAAATAAATCATTGCTTGACTAAACTAAACTTTGAGTTTAGTTTTTATATTTAAGGAGTTTAGATGTATAAAAAAATAAGTTTAGATGATAAAGAAATCGAAGAATTAATTAACCTAGAAAATCAACGTTTAGAAGACCATGTAGAACTAATAGCATCTGAAAATTATGTTTCAGAAGATGTTATGAAGGCAAATGGTTCTTGTTTAACTAATAAATATGCAGAAGGCTATCCTAATAAAAGATATTATGGTGGTTGCGAATATGTTGATAAAATAGAAGAAATTGCACAAGAAAGAGCAAAAAAATTATTCAATGTAAAATATGCTAATGTTCAACCATATTCAGGAAGTGTTGCAAATGCTGCTGTATACATGGCAATGGTTGATCCTGGAGATAAAGTCTTAGGATTAGATTTAAATTCAGGAGGACACTTATCACACGGATATAAAATTTCGTTTAGTGGTAAATTTTACGAAGCACATACCTATTCTGTTAACGATCAAGGTTTATTGGATTACGACAAGATATTGGAAATTGCAAAAGAAGTAAAACCAAAAATGATAATTTGTGGATATTCTGCATATTCTCAAATTGTTGACTTTGCTAAATTTAGAAAAATTTGCGATGAAGTAGGTGCAAAATTATTTGCCGATATTTCTCATATATCAGGATTAATAATAGCAGGATTACACCCATCGCCTTCGGGTTATGCTGATGTTATAATGACTACAACACATAAGACATTGCGTGGCACTCGTGGAGCAATAATTTTAACTAATGACGAAGAAATTTTTAAAAGAGTTAACAGTGCGGTTTTTCCTGGCGTTCAAGGAGGACCTTTGTTGCACCAAATAGCGGCAAAGGCAGTAAGCTTTAGAGAAGCATTGCAACCAGAGTTTATTGATTATCAAAAGCAACTATTGTTGAATTCAAAAATTATGTGTCAAACATTTTTGAACAAGGGAGTAAAAATTATCTCTGGCCTTACTCAAAATCATTTATTTATGATTGATGTAAAAACTAGTTATGGAATAACAGGAAAATATGCAACAGAAGTTTTGGGTAGAGTTAATATAACCGTGAATAAAAATACTATTCCAAACGATAGTGAAAAACCAACCGTTGCTAGTGGAATAAGAATAGGATCTGCGGCAATGACATCAAGAGGATTAAAGGAAGATGAGTTCATCATTTTAGCCAATTTAATTGATAAAGTTCTAAGAGATCCAGAAAACGAAAACTTATTATATGTAGTAAAGAAAGAAATTGCAAAATTAACGAATTCATTCCCAATTAAACGTTCTCATTTAGTTCGTTAGAAGTAGGCATATGATATTAGCGGAAATAAAAAATTTAATTAAATCTTCAATAAGTATTATTCCAGGAATAGTTGATTTTGTTGAATTAACTTCAACAAATAATATAAATATTGAAAATGATGGTTTAATCATTGAGGAAGATCCACAAAATAAAAACATTATAAATATAACAATTGGTTTGAGAATAATGAGCAATTTAAGTGCTAAAAATATCGTTGAAGGAATATATGAAATCATTCCATATAACTTAACTTCTTACGATATAAAATTAGGAACATTAACAATATATATTAAAGGAACTAAATAATGAAAATTATTACAGGAAAAGAGTGAAATAAAGCCGTATATAATGGTTCAATAAACATAAATAACAAAAAAAACGCAATTAACGCTTTAAATGTTTTCCCTGTACCTGATGGTGATACAGGAAGCAATATGTCATCTACAATTTCTTCTGCATGTAATTCTGAATGCTTAGAAGACGATTGCAGTATTGAATCAGCTTCGAGAATTGTTGCTCAAAATATGTTAATGTCCGCCAGAGGAAATTCTGGTGTAATTTTAAGTCAAATTTTTAAAGGTTTTTCTAATGGGTTTGCATCTAAAAAGTCTGCTAATGCTTTTGATTTAGTTAATGCTTTCGAAATGGCTGCTAAATCAGCTTATGGTGCAGTTTTAAAACCTGTTGAAGGAACTATTTTAACCGTTATTAGAGAAGTTTCTGAAGGTTTAAAAAACCAAGTTGTGGTTGATACTACTATTGAAGAAGTTTTTGAAATTGCTGTTAAATTAGCAAGAAAAAGTTGTGATGGTACTCCTAAATTATTAAAAATATTAAAAGAAGTAGGTGTTACTGATTCAGGTGGTGAAGGTTTATATGCAATATTGCAGGGAATGAGCGCATACTTTAGTGGTAATAAAGTAGAACCTATTGTTGCAGAAGAGTCGGTTGAAAAATTTATTTCTGATGAAGAGGTATATGAAGGCGAATTTGGATATTGCACCGAATTTATTTTGGAATTATATAAACCAAAAAAATTCGACAAAGATTATTTAGTTCGTAAACTAGAAAAAATTGGTAATTCAATGGTTGTGGTAAATGATGACAAAATTTTAAAAGTTCATATTCATACCCCTAAGCCCGGTGATGTTTTAAATAGCGTAAATTCTTTGGGACAATTTGCAAAAATTAAAATAGATAACATGACTTTACAAGCAAACAATAGTAAGAAAACTGCAAAGAAAAAACAAACCGAAGCGCCAAACAATGTTGAATTAAAAAATAGCGCCATTGTTTCGTGCAATACAGGCCAAGGAATCGTAACCTTAATGAAAGAAAACGGTGTTTCATATGTTGTTGATGGTGGTCAAACAAACAACCCCTCAATTCAAGATATTGTTAATGCAATAAATGCAGTCAATGCAAAAACGGTATTTGTATTGCCAAATAACTCAAATATAATTTTATCTGCTCAACAAGCCGGTACTATTATTAGAGATAAGAATGTAATTATTATTCCAACTAGAAGTCAAGTTCAAGGATTAAATGTTGCATTGAATTATAGTTCTGAAAATAGTGATGAAGAAAATCATGAATTAATGAGTAACAGTTTAAAACATATAAGATATGGAGAAATTGCTGCCGCCATAAAAGATGCTAAATTAAACGGAATAAAGGTTAAAGCATCCGATTATATGGTTATTGTAGAAGGAAAATTACAAAAAACATCAAAGAGTCTATTCAACGCAGCACAATTATTAGTATCAGAATTAGTTTCGGATGATAGTCAAGTAGTAACAATAATATATGGCCAAGATGTTTCGGAAAATGATGCGCTGGAATTACAAAATTATATTGAAACAACATACGACCTTGTTGTAGAAATTTATCAAGGTGACCAAGGAATTTATCCATATTTAATCTCAGTCGAATAGGAAAAAAAACAATGAAAAAAATAGTATTTGATTTATTGAATAATGATAATGGCGAAGAACAAGCGATTTTGGGTGCAAAAAGATTTTTACAAGAAAATTCAGATTATTTTTTAATTTTGGTTGGTCATGAACAACTTATCAAAAAACATTTATCAGATTACATTGATAGGATTGCCATAATTAATAATGAAACAATTGTACACATTGAATCTAGTCCAAGAGACGTATTGAGAACTCAAAATTCAATGCTAGAGGCTTTTAAACAACTCAACGAATCAAATGCAGATGGAATATTGAGTAGTGGAGATAGTGGCTCGTTAATAACATTATCATCATTGAAAGTTAAAAGGCTTCTTGGGATTGAAAGACCAGCATTCATGCCTATTCTGCCAACAACAACGAATAAGAAAATGTTATTAATAGATGCAGGGGCAAACATTGAAATCAAACCAGAATATTTAAGTCAATGGGCTATGATAGCAAGTGAATGATATTCAATATTGTTTGGAAGCAAAAAGCCAATAGTAGGACAATTAAACGTAGGTGTCGAAGAATACAAAGGTTCTGAAATAACAAAGAATGCTAAAAATATATTAAAAGAAGTTTCTAAATCTGCTAATTTTGTTTTTAATGGTTATGTTGAAGCAACAAATGCCATTAATGGAGATATAGATATATTAGTGGTTGATGGATATGGCGGAAATGTGTTTCTAAAAACATTAGAAGGAACCTTTATTGCTTTTTCAAAATTATTGAAAAATATTTTATTAAAAAATTGAAAAACAAAATTAGCGGCATTAATTTTAAAAAGGCATCTAAGGGAATTTAAACAAAAATTTGATTATAGAAATACAGGCGGAGCATTTATTATTGGGCTTGAAAAAATTGTTGTAAAGGCTCATGGCGGAAGCGACGAATTAGCATTTTATAACGCCCTTAACCAAATAAAAATAGGTATAGAAAACAATATTTCAACAATTTTAAATAAGAAATTCAAAGAGGCTGATAATGAAGATTAATTTTGAAGAAAATTTACAAAATGCATTGCTAAAATTTGGTATAAATTTTCAAAAATTAGATGAACAAAAAAGACGTTATTTTTGTAATGCTTTTACTCATAGTTCATACAGCAACGAACATAAAAATTTAGAAAGTTATCAATATTTAGAATTTTTAGGTGACTCAGTTTTAGGTTTTGTGGTTTCTGATTTCATTTTTGATAATTATAAAAATTTCGGTGAGGGAAAGGCAACAAAATTAAAAAGTGAAATTGTTGACAAAAATGCGCTTGGATTCTTATCGAGATATTATGGTTTTCCAAACTGTTTGAGGACGGGCAAAAGTGCATTTGCAAACGGAAAAGGAATTAAATCAGATTCTGATCTAATTGAATCATTCATTGGTGCTTGTTATAAGGCCTGCGGTTTGGAATTTACTAAAGAAATTATTTATCATTTTATTGACAAAATATTAAAAAAATTTCACAATTCAATCAATGAACACCCTAAAACAAAATTGCAAGAACTTGTACAAAGCAATTGAGGTTGTAGGCCTAGATATGAAGATCAAGCCTTAAATAACGGCAAATTTGAAAGTAAGGTCTTTATTAATAATAACTTATATGGTGAAGGTATAGGTCAAAGTAAAAAAGAATCCCAAACAAAAGCAGCAAGTGATGCTTTAAAAAAAATGGCAAAAATTTAATTTGGAAGGAAAGAAGTATTTATGAAGTTAATTCAAGTTGAAGCACACGGTTTTAAATCATTTGCAGATAAAGTAACCCTAAAATTTGACGGTGGAATTGTTGCAATAATAGGACCAAATGGGTCAGGAAAATCAAACATAAATGATGCAATCAGATGGGTGTTAGGTGAAACTAGTTCCAAAGTGTTGCGTGGAGATACAATGGAAGACGTTATCTTTTCAGGTTCTAAGACTGAAAAAGAAATGGATCGAGCAGAAGTAATTTTAACATTTGACAATAAGGATAGATCTTGTGATATCCCATATGATGTTTTTACTATTTCTAGAGTATTAACTAGAGGAAATGGTTCAAATGAATATTATATTAATGGTGAATTAGCAAGGCAAAGAGACATTAAAGAAATTGCAATGCAAAGTGGTATTTCAAAATCCAGTTTAGCAATCATAGGTCAAGGAACTATTTCCAACATTGCCGAATCAACTCCTGAAAAACGTAGAGAAATATTTGAAGATGCTGCTGGAACTTCAATGTACAAAAGCAAGAAGATTGAAGCACAAAGAAAATTAGAAAAAACCCAAGAAGCATTGGAACAAATTTCAATATTAATTCAAGAACAAGAAAGACAATTAAAGCCATTGCAACGTCAAGCTGAAAAAGCAAAAATTTATAAAACAAAAGCAGAACAATTAAAAGAAGTTGAAGTAGCATTATTGGTTCATGATTTCATGGATTATTCAAATAAATTAGAAAAATTAAATGTTGAGGGGCAAGATTACAATCTAATTAAAGAAGAATTAGAAACTAAAATAAGAGTATACAACGAAGCAAGCGAACAAAAAAGCAAGTTTGTTTTAGAATTAGAAAATAACATAAAAAAGATAAGCGAAAGATTAAGCGAAATTAGCGAAGAAATTACGACCCTAGAAATAAGAAACGCAAAAGAAGCAAAACACCGCGAAATGCTTTTAAGTGGCGAACTAACTATATCCCCAAAAGAAAAATTAGCAGCAATGAAAGAAGAATTGAATGCATTAAATACTAATATTTTAGGGTATAAGACATTCTTAGAAACCAAAAAGAAGGAATCTGAACAATTATCTCAATCAGCTTCAATTAGATCAACAAAAATAAATGATATAAAACGAGAATTATCCTTTAAAAAAGAAGAACTTAATAAGATAAAATCAAAATTAGATGTTATTGAAGAAATAAAATCTAAAAAGACAAATTTGGCTAAAGGAACACATAATATTGTTGAAAACTCTCATTTATTTAGAGGATATAAAGCATTGGTTAGTGAATTAATTGAAGTTAATGAAAAATATGCCTTAGCTATTGAAACAATTCTAAGCTCCGCTATGCAACATATAGTTGTTGACAAACCAGAAACAGCAGTTGAAGCAATCAATTTCTTAAAATCAAATAATGGAGGTAGAGCAACTTTTATTCCTTTGTCATCAATTAAACCAAAATTCGTTCCTGAACAACATATTGTTGTTGCTCAAACCCAAAAAGGTTTTCTAGGAATTGCTTCGGAACTTGTTTCAACAGAGGAACAATATAATGTTTTAAAGAAATTCTTACTAGGCAATGTTTTAGTTGCAGATAACATTGAAAATGCAAATAAATTATCTAAATTGCTAGAAACTCGTTATATGGTCGTAACGCTAGATGGCGACATCATAAGAGTTGGTGGAGTTCTTTCAGGAGGACAAGCAAGTCAAAACGTTTCCACATTAGCAGCTGATGAACAAGTTAAAAAATTACAAGACCTTATTCCAGTAATTAATGCAAAAATTTCACAATTATCAAATGAGTTGTTTGAATTAGAAAAAGACCAAAATAAAGAAACATCATTATTAACAAATATTTCTCTTGAAAGAGCAAAGATTGACAACTTATATAGAACAACATTGGATAAGTTTGATTCATTGCGTGAACAATACCGTAGTGCTTCGCAAGAAGAATATGAGGCAGAAAACAACAAAAAAATTGATATAGTAGCAGCAATTCAACAATTAATGCTTGAAAGAAGTGATTTATCAGCTAAAAAATCATCTCAAGAAGATATTTATCATAATCTAAAACAAGAATTACAAAATCTTACCAATCAACGCTTTCAAGTTAGTGAAGAATTAAATAATTTGATTAAAGATAATGCAAACAAGATAGCAGAAAAAGCAAAAGCAGAAACAATTATTCAAAATGCAAAACAAAGATTGTCAGAACAATATGGAATGCTATTTGAAGTGGCAAAACAATTTTACAATCCAGATATAGATTTTGAAGTAGCAAGAAAAACAGTTGCTGATTTGAAATTGGATATAAGAGAGTTAGGACATGTAAACCTTGATTCAATTAACCAATTAGAAGAACTTGAAACAAGATATAAAGAAGTAAAATCTCAAGAACAAGAAATAATTTCAGCAAAAACCACCATTGAAGATGCAATATCAGAAATGGATAAAATTATTATTGAAAGAATGACACAAACAGTTACATTAGTCAATGCTGAATTCAAGCATGTGTTTGCAAAAATGTTTGGCGGAGGTATGGCTGAAATAAGATATACTGACCCTGAAAACATTCTTGAAACAGGAATTGACGTCATTGCTCAACCTCCAGGAAAATCTATTAAGAATTTAAAATTATTTAGTGGGGGTGAAAAGGCACTTATAGCTATTTCATTACTATTTTCAATATTAAAAGCTAAGCCATTGCCATTGTGTATCTTAGACGAAGTTGAAGCAGCACTAGATGAAGCAAACGTAATAAGATTTGCTGAATATTTGCACACTCTAAAAAAAGATACCCAATTTATAGTGATTACTCACCGTCAAGGAACAATGGAAAGAGTAAACAAACTTTATGGCGCAACGATGCAAAAAAGAGGGGTTACAACATTTTTTGCAGTAAATCTTTCAGACGCAAAAAAGGCAATTGAAAACAACAATTAATTTTTTAAAAAAACGTTAGCTTAAAAAAGAGCTAGCGTTTTTTTAAAATTTATTGCCAACTAACGTTTTTTATTAATTAATTTTTAGTAAGTTTAAATATAAAAAGAATTTTATTAATATTTTTGATATTAAAACCTAAAAACGCAATATTTTCAATATTTTTTAGTTAAAAGTTAGATAATTTTTATAGTGTATTGTTAAACAAATACCATTTATGTCAATGACTAATGTTATATTACAATAAACAAGGAGAGCCTTATGTACGGGTTAGTTTTTTATTTAGATAAAGAAATATTAAAAAAAGAATACTCAGATAGTAATCATAAAGAAGCTTATGAAAAAGTAAGAGATATATTGAGGCACTACGGATTTCATTGATTATCAAATAGCTTTTATTTCTCGAGGTCAATTAATAATTTATTACAAGTCTATAAAGCAATAGAAGAATTAAAGACAATTTCGTGATTTATTAAATCATTAGTTAGCCTACACATTTTTAAAATGGAAGATTTATCAGATTTTACTGAATATTTAACAGGCGAAAGTGAAAAAATAGAAGAATAATTAAAAAAACTTGTAATGTATTAAATTGCAAGTTTTTTAATTAATTGTAGCGAATCTTTTGGATTAAAATTAAATAAAAGGCAGCAAAAGATCTCAAATGAACAATATTTTTTATAATTAGTTTATTGGAATATTTTAATATTTAAAAAGTAGCAATTGTGCTACTTTTTAAAATTGATAAATTATTAGTTTAATTTATATAGATGATTTGGGTTTTCCTTCAAAGCTTTTTCTAAAAACGATTGAATATCATTGTTTTCAATTTTAGAAATAAGAGTTTTAGCAGCTGATTCCCCCGCACCAATAAAGTCAAATGATTCAGGACTAAATGATTTCATTGTTGAAGGAATTTCTATTGTTTTTATTTGATTATTTGCCAAGGCTCCGCTTTCAACTAATTCAATGTTTTTTAAGTTTATGCTTGTAAGGTTATTTGAAGCAAAAACACCTTGTGTAAGAGTTTTTAAATAAGGTAAATCTTCTAAATTTACAGATGTAATGCCACTGTTTGCAAAGGCTTTTTGTCCTAACTTATTTAAAGATGCAGGCAATTGTAATTTTTTAATATTTGTATTTGAAAAAGCTTCAGCATCTATTTGAGATAAGTCGTTGCATTGAGAAAAATCTACTTCTTCTAGGTTACTTAATCCATAAAAAGCACCTGCGCCAATTGATTTTAAGGTTTTTGGCAAAATAATTTTTCTAATATTTATTTTTTTGTTGTTATTTGAAGAATTAGTTGTAACTAATGTTGATAATTCAGTTGGTAAGCCAGCACCATCGTCTGCCAAATTTTGCATAACTAGATAAGAAAATGATCCTGCGGGTATTGAAGTTATATTGGTTTTTGATAAATCTAGCAACCCTGATTCAGCAAATCAAGTTTGATTGTGATTGGATGAGTTCTTAGGGTCTTTTAGTGGGCCTTGCACATGATTTAGAGCAAAAGCATTGAACTTTTCTCCTCCACAAGATATTGCTGTTAATCCAGCAATAAATGGAGCAACTAAACCAAAGCCTAAAAATAAAAATTTTTTGTTCTTTTTCATAGTCAAAATTTTACCATAATTTTGTTATTTTAGTTTTTTATATTAAATGTCAAAAATAAATTACTTAATTTATATAATTAAATTAATAAAATCATGGAAAAGTTTAAATTAGTTTCAAATTATAAGCCAGCCGGCGACCAACCTCAGGCAATAAATGAGTTGGTTCGCGGTGTTTTAGAAGGTAAAGAGCATCAAGTTTTATTAGGCGTAACTGGTTCAGGAAAAACATTTACTATGGCAAATGTCATTAATCAGTTAAATAGGCCTACATTGGTTATAAGCCATAACAAAACATTAGCAAGTCAACTTTATAGCGAATTAAGGGAATTATTTCCTGAAAATAGAGTTGAATATTTTGTTTCGTATTTTGATTTTTATAAGCCAGAAGCGTACATTCCTAAGTCTGATTTATATATCGAAAAAACAAGTAGGAACAATAAAGATTTAGAAGCAATGAGGATGTCTGCTCTTAATGCCCTAAGCATTCGAAGAGATACTATTGTTGTTGCATCTGTTGCTGCAATATATGCCGAATTTAACCCCAAAGAATATATTCAAAGTTTTATGCCTATTGAAAGCCATATGAAAATTAAGTTGCGTGATCTTTTAACCAAGTTGGTTAATTTAGGTTATGAAAAGCATAATGGTGATTTATCTTCTGGACAATTTTTCAATAAGGGGGAAGTTATTGAAATTTGTCCCGGATATACTTCAGAAAACAATATTCGTATTGAGATGTTTGATGATTATATTGAAAGAATTTCATTAATTAATCCTTTAACAAAGTCAATAGTTGAAGTGCGTAAGTCGTTGACTATTTTTCCTGCAACTACCTATAACGTAGAAAAAGGTTATATTGAAAATATTTGCAATAGCATTGAAAGTGAATTAAAAGAAAGGGTTGCCTATTTTAAATCACAAAATCAACTTTTGGAAGCCCAAAGGATTCACGATAAAACTTTAAATGATATTGATTCATTAAAGGAATTTGGTTATGTTAAGGGTATTGAAAATTACGCTCGATATGTTGATAAACGCGAACCCGGACAAAAACCATACACAATATTCGATTATTTACCAAAGGATGCAGTAATTTTTGTCGATGAAAGTCACCTTATGATTCCTCAAATAATAGGAATGTACGAAGGAGATCGTTCTAGAAAAGAAACACTAATTAAATATGGTTTTAGATTGCCTTCTGCCTTGGATAATAGACCTTTAAAATTTAATGAATATGAAGAATATAAATTTCCAAGAATTTATATGTCAGCAACTCCTGGCGACTATGAAATAAATAAGGCAAATGGTGAACTAGTTACTCAATACATTAGACCAACAGGCCTTTTAGATCCCATTATTGAAATACATTCAAAAGAAAATCAAATTCAAGACATTCATGATTTATTAAAATTACAAATAGAAAAAAAAGAAAGAACTTTAATTTTGACTACAACCAAAAAGAATGCCGAAGAGCTTTCTTTATACCTTTCTGAATTTAAAATTAAAAGTGCATATATTCATGATGGATTTAAAACCTTTGAAAGAAATGAAATCTTAAAAGGTTTAAGAATGGGAAAATATGATGTTGTAATTGGAATTAATTTATTGCGTGAAGGTATTGATTTGCCAGAAGTCAGTTTGATTTGTGTATTAAATGCAGATTCTATGGGGCTAATGCGTGATGCTAGAAGTTTGATTCAAATAGTTGGTCGAGCCTCTAGAAATGATCATGGAAAAGTAATATTTTATGCCGATAATATTACAACCAGTATGCAAAAAGCAATTGTTGACAATGAACATAAAAGAACATTACAAATTAAATATAATAAAGAACACAACATTACCCCTAAAAGTATTATTAAACCAATACCCGCACCTATTCAAGGCAGTATTTTATTAGAAGATAATAAGGCAATAAAAGAAGCCAATAGAACTATTCATAATAAAAAATATATTGATAATATGATTAAACATATGAAAGAATTAGCAAGGGCAAAAAAATACGAAGAAGCTATTGAAATAAGAGATTATTTGATTGAATTAGGTGTCGAATTTGACGATTAAAAAAAGGACTTTGTCGTCTTTTTTTTTAATCATTTTTTGTTGCTTAAAAACACAAAAAAATAAAAAGGAAGTGTTTGAAAATCAGGGGTTGCAAAATCATAAAGAGATTTTTTCTATCACCCTAATTATCCTAAATTAAAGATATTTTTTAAAAAATTCGCATTTTTTTGATGCAATGTCTATTCTTAAACAAATTGATAATCTTTTACTTAAAATTGTAAATTAAATAAAAGAGTATTATTTATGGCAAAAATTAATGGTTCAATTATTTCAGAATATGAAATTAAAAAAATTAAAAATTTAGATTTTATTTCCAAAAATTTGCAAAGAAAAAATAGTTATTTGCAAGAAAAAACAATGTTATCAATTCCTACAATTTGTAGATATAAAAAAATTATTAAAGATAATATGCAAGGTAGCAAAATTGAAGTAAATATTAGACATAAAAACTTTAATAATCAAAATGCAAAAATAATAGAAAACGATTTTGCAAAAGACTTATTTTTGAAATATCAACTTGAAAATCAAAAAATAAATTCAAATGTTACTAAAAAATTTATTACTGTCAAAGATTTTTATGAAGACCTTAGTGAAGAAATAAGGTCAAAAATTTCGCTATCATATTTATATAATGCTTGATTAAAGCATGGTTTTTGTAGTCCTTATGCTACCCATAAAATTAAAAGAATTGCAAAAAATATTTCAAATTTGAAAATTAATGACAGCAAAATTAGCAATAGTGAAAAATTAATTTATAAGGCGAATATTTTATTGATTGAAAACACTAAAAAATTGTCAATATATTCAAATAAAAATCAAAATTATAAATTTGGTGAAATTATTGAAGTAGATGCTTGCCAAGATATATTTTTCAAAACCAATGAAAAAATTCATATTTATCATGCAATTGATGCTAAAAGCGGTGCATTAGTTGCCTTGTGAGCTGAAAAAGAAGAAACCAATAAGGGATATCAAAAACTTTTTGAGCTAAATCAAAAGTATTTAGATCAACAAAATAAAACAACAATTGAAATTATTAAAAATCAAACAAAAATTTATAATGAATTAATAGAAATTAACAAAAAATAAACGATTCCATTTGGCAAAAAATATAAAAAATCTCTTACTAAATTAACTTATAATTTTTTTAGTAAAAGATTATCAATTTGTTTAAGAACAAACATTTGAGAAGAGGCGAGTTAATTATGGCTAATGAGTATTATAATATGTGTGCCTTAGATGCACTCAAATTTGTGAAAAATAGAAATATGTTATTACCAGATATTCAAAGAGAATATGTTTGGGATACAACTGATATAGAAAAATTATTTGAATCTATAGTTGATGATTATCCAGTGGGTTCTTGTATATTTTGGAAAACAAATAAAAAAGTACTTAATGATGAAAAACCAAATCTTTATTATTTTATTTCTAAATATAAGCAAGGCGTAACTAAAAATGAAAAAGCACCAGAATTTGTATCTGATGAAACAGATTATTATATAGTATTAGATGGACAACAAAGAATTACATCTCTTAATATTGCATTGTTTGGATCGTTTACATCTAAGAAAAAAGGTAAAGGATATAGTGTAAAAAATTCTAAATCATGGGTAGAACGAGAACTATATTATAATTTATCTTTTTACGACAGCAATGAATTGGATGATGAAAATCCCATTAAAAGATTTGTATTTTTGTCTGAAGAAGAAGCAAAGAATGGTAATTATTATAAAGTTAAGCAAATAGTTAAAAGTGATAACTTAACTGATTATGTTGATACAATATCAAATTTTAACAAAAATGTAAAAACGGATTTAATGTGTTTGTATAGAAGAATTATGGAAGCGCCTGCCACTAGTGTAATTCATTGTTATTGCATTGCAGAGAATACATATGATGAAGCACTAGATATTTTTGTTAGAGTAAATTCTACTGGAAGAAAACTATCAAAGTCCGATTTATTATTTTCTACATTAATTGATGGTTGGAAAGAAGGGAAGGAAAATATAGACACCTTATTGTCTGACTTAAATTCAGCCGGAGATGGATTTTCTTTTACAAAAGATTATTTAATGCGATTGGCTTTGGTATTAGTTGATGCAAATACTAATTTAAAAATTAATTCGTTAACTTCTAAAACAATAAATGAAATCAGGGATAACTGGCAAAAAATAAAGAAAGCGCTAGAAAAATTATCTGAATTATTAGTTTCAATTGGCATGTGTGACGAAAATATGACCTCATATAATGCAACCATTCCACTGGCATATTATTTTTACAAAGGTGGTAAAATTGATACAGCAGAATCAAAAAAAGAAGCAAGAAAGTTCCTAGCCGTTTCAATGTCAAAAGGATTGTTTGGTGTAGCAAGTAATGATTCTTTGAATCAAAGCAGAACCCTCTTAAGTCAGTAAATGAAAAAACTCCATTTTCATTAACCTTATTTAGTAGTATTACTTTAACGGGTAATAGAACATTTACTGCATCGAAAGATGATATTGAATATTGGTTGACGAAGTTTGAAAAAGGAAAAAACACATTTATAATTCTTTCTTTGCTTTATCCTAATTTAAAGTTAAGTCAAGTTGAGTTCCATCAGGATCACTGTCACCCTTATACTTTATTTGATGATAAAAACATTAAAAAATTAGGATTGACAGAAGATAAAATAAAAGAATGGCAGTTTAAAAGAAATTTAATTCCTAATTTGCAGTTTTTAGTAGGTAAGGAAAATGAGTCAAAAAATAAAACGCCTTTATCAGATTGGGTTAAAAAAGGAAATACCTTTGAATATTGACCAGAAAATGTTTCTCTTGAATTATCCAATTTTGATGAATTTTTTAATAAAAGAAAATCGCTTATAAAAAATAAGCTTTTGGATATTTTTGGTTTAGAAGCAAACCAAAAATAAATAAATGTATAAAAATCTAGATTTTACTCAGTTCTAAATAAATTAATTTCTATATGAAGTGGAATTACATGGTTCTTTTTTGCTAGAATATTGTAAATATTCGTATGCTTTGATATAGTATTAATGCAAGCCAATGTGTATAGTGCCTTGCAGTACCTATGACCGATTAATGGTGGAGTGGTGCAAAAAAAATTATTTTTCTCTGATCTCAAAGGGTTGATGAAAGGAGGAACTTATTATGTCATCAAACAAAGTAGAAAGATTCGACTACTCTAAATACGAATCAGTGTCAATAAACGACCATGATACTTTAGGACAATATCAATTCTGGGGTATTCATCCTAAAGGCGGAAGATTAAGAATCGATACAAAACGTTATAAAAAAATGTGTGAATGGATAGGAATCACTGACTTTATGCCAGATGGTTTGTTTGTGCCAAGAAGCACTGCTTATTTTACACCAGTAAAAAAGGAAAGATACGACTATAAAGTAAATCTATTTAATGACTTATTAGATGGATTAATTCATGATTGGGAGTATGAATACAAACCATTATTTAAATCCATAAAGACACCAGATCAAGTGGCGAGCGATAGTCGTCTCGGAATGCTTGCTTTCACAGGTTGTTCAGATGACTATGATGAAATTGACATAGAGGCAAAGATGAATGGCTTTAGAAGAATAGAAAAATATAATCAAATTATTAATTCTTTACATTGTCAATTTATATTGAAGATTGGTGCGGAAGTAGACCGATTTACCTTAAGGGTAATGACCGAACTTGGATATAAAGGTACTGACTACGATTTTAAATCATTTTGTAAATTTTCTGATGGATTATTAAGAAGTAAAAATGGTATGCCATTAAAAGATTTAAAGAAGTTTGAAGCGTACAACATGCTTCATAAAATATGCAATTTTCTAAAACATAATTCAAAAGAAGCATACAACACAATGAAACATTTGTATCCAGATAATGTTTGTTCCAAAGAAAAAGGAACCGCTAGTACGGATTTTGAAAATGGTATGTTTGCAGGTGATTGGATTATCATTAAAGAAGGTTATATTGATAAGACATTAAAACAATTAAAAATATTCTTCACTGATTACTGCAAAGTCTACTTGAAAGAAGACGTAGATAATGCGTATTGGAATTATGATGATTATTTTAAAGATGCAGTTTCTCAGATGCGTTATCCATACGATTATTAGGGTATTTAATTAGCATTATACTAAGATAAACTAGGGACAAACTTCGGACCACAAATCTATTGATTTGTTTTATAATGAAGTAAAGCAAATGAGGAATAGAATGAACACCGATATTAAGGTGCCGAAGGTCAAAGGGATGTTACTAGGTAGAACACCTTTAGGCCATAAAACTCTTAGAGATGAGACTATCATCGCTAGAATCATTGAAGAAGACGAACCCGCTACTGTGATTGACAATGGCTGGGACACAGTTATTAGGTTGCATTACAAATTAAAGTCAAAAAGGAATAAAGCATATAATACTAAAGCACTAGTGTGGATTGAATTTCTAAAGTCATTATTGTGGTTAGATGAAATACCAGCTGTATGGTTTCCAAAAGAAAAAGGTTTTATTAAGTATGGTGATGTGGTTTTTGTGATGTTAAAATCAGGAGAGTTTTTCTACTCAAGAAGCATCGCACATAGATTATCGCCACTAGATCTAAAATGGAAATTGCATAACCAAAAGAAAAACACCGAGTATGAACTAGGTGACATTTTTTGTTTATCAAGTGTTAGTTCCATACGATTTATACGATGATGAAAAATACTATCGATTCCTAAAAAAAGATAAAATTGATATTGATGAATAAGCTCAGCTAGAAAATAGTTGAGTTTTTTTATTATTCTTTGTCCGTTTTATTTGGTTTTGTGTAGAGCTCTTGAAGGGATAAGAAATCTCTTCGAGGAGGTTCTTACAATGATTATCGAAGAAAAAAATATGATTATTAAATTAAGTGTTGATGGATTGGACTATAGGCAAATAGCCAGTCAATTAGATTTAAATAAATCTACAGTATCATCTTTTTTCAAAGGGAAAGTAAGAAAAAAATACGAGAGGTTCCTCGACCTTTTGTAAGAATTTTGATGCGGTTTTATTCAATATAAATTCAAGACCAACTAAAAAGTTTTGTTCTGATAAATGTCGTTTGGAGTGGTGGCAAAAAAATGAGAAATCTATAAATAAAAAGGCATTCTATAAGCTTGAGTGTTCTTGCTGTGGTAAGGAGTTTATTTCTTACGGACATAAGGAAAGAAAGTTTTGTTCGGGACTGTTTAGAATTTTGTGTATAGTCAATTCCTTTCTATTTAAAAAAGGAGAAACTTTACACAAAATTCTAAACAGTCTCTTTTAAAATATTTAAATTAGGAAGAATTGAATTAATCCTTCGTTCTAAATCATTAACAATTAATGATTTATCGCTTTTGTTTAATGGCAAGGTTTCTTGATATATATCTTGATCTATATTAGCTTCCAAAATCTCTTTATCAATAATAAAAACATCTTTAGTAATTGCTACACCTTTATGATTTTTTCAATATTCGTTGGTTTGATAAGAGCTGTTATCAATTTGTAGGTTATATTTAGTTGTGTTTTTTCTCGCGGTTTGATGTCTTATAAATAATACGTTTTATTTTCTGTATTTTCAAAAATTATCAAAGGATGAATTTTGAATTTTGTGTCGATATTTAGATGAAAAATATCTTCTAATAAGGGGTTAGTAATTTTTAATGGTTTTCATAGTTCTAAACTCATATAAAAATTATACAAAAAAGAGGAATAAACCTCTTTTTAAGACGAACCAGCCTAGAGTAATTGTAGCTACACGAACCAGCACGCTACTGTTCGATTACTTACGTGTATCTCTCTAGGTTCATATGAGTATATTACATCATAAAACCATTTAATTTTATAATATTTTTAAAAATAATTGAATACATAAAGTTAATAACAAAATAGCACTCAGAAACAAAAGCGCTATTTTTGCATATACTCTATAACATCATATTTGGGTAATTACCTTACTCTTCTTTAAAAAATGTCATCATAAAGGGTCTCATATTTTTAATTATCGGGACTTTATAACATTTTTTAATTACTTTATTGTTTGTTCTTAAACAAATTGATAATCTTTTACTAAAAAAATTATAAGTTAATTTAGTAAGAGAATTTTTTAAAGTTGTGATAATTTCTACTAGATTTTATTTTTATATTTAATCCTTGCTCTAGGTCATATAAAAAGAAGTCTATATTCTCTTTATTAAAACCCGATCGTTTTAAATTTCTGCGATATTTATCAATTAATTCATTTATATGCATCTATATTTTCATAAACTTTTTGGAAATTCACAAAGTAATCTCAATTACATATTCTGTTTTTTGAAAATGAATTTAAAAATTTTTCAAATCTTATTTGCATAGATCCTCTTTATCAAAATTAGTAATGATTACCTCTTCGACATCGCCTCGATTTTTACCATTTCTATTAATGTTTCTTCTTGCTTTAATTATATTAATATTAAAATCCCTGTATAAATTGCAAACAAAATCAGTTTTGTGATTACTTAATATTACATAAACGCCCTTTTTTGACAGTTCCTTAAAAACATTAGCAAGTTTAATTTGCTCGTTTTTTCCAAAGGTTTCATTTGAATAAGAAGTAAATGAATTTTTATTTTCTAAGGTATCATAAGGAGGGTCAAAATAAACCAAGTCGCCAGCTTTTGCATCTTTTATTGCATCTTCAAAATTTAAATTTGTTATTTTGTAATCTACATTAGAAAAATATTCAAAAATAGAATTATAGTTTTCTTTATTATAGCAATTGACTTTTTCTTTCTTTCCTGATGGAACATTAAAAAAACCATTTTTATTTACACGATATAAACCATTAAAACATGCTTTATTCAAATAAATAATTCTGGCAGCCTTTTCTTCATCTGACAAGTTTTTATATTCGGGTTGTCTATCTAAATTTCTTATTTGATAATAGTATTCGTTGGAATGCTTACTGGTATGAAGGCCTAAAAGTGATTTTAATTTATCAAATGATTCCCTCTTTGTAAAACACTTGTATGCTAGAATTAGTTCTTTATTAATATCATTAATGCAAAATTTCTGGGCAAGAGACTAAAAAGTAATGAACCTCCGCCAATAAATGGTTCATAGTAGTTATTAAATTTTTTAGGTATAAAATTTAATAATTTTTCTAAAATTTGTGTCTTTCCACCTGCTCATTTTACAAAAGGTTTTGTCACTTTTTTCCTTCCTTAAAATTAATTATATCTTTTAAATTCAAAATTAACTTCATCATTGTTATTTACTAAATTTAATGAATAGATTAGATTTTTTGTAGTTTGTTTTTCTGTGTCGTATCATTCCATTGTTTTTCTAAAATTTGTTACATTGCGTTTGTTATTTTGTTGGTGGATTCAATATCCGTTACTTCCAAAGGTTAAAAAATAAGAATTGTATAAATCAATAATTTCATTAATTAATAATTTTGCTTTAGGCGAGTTAGTATTTTTTATTCCTTCTATCAATTGCACAACTTGTTCATACAATGATTTATTTTCGTCATATTCAACGTTTGCAAGCAATGATAATTCCTTCATTTTTTTATCTAATATATTTTTGCTTGAAACAATGTTTGATATTTCATCCGATTCAATTTTATTATTATTATTTTTATCAACTCAAAGTTTTAAATTTAAATAGTTATTTTGATTTGTATAATCTTTTATTTCATTAATTCCAATTGAATCAGAAAAATATGAAACATATTCATAATTTTTAACATTATTTGAATATATGCTTTCTTCATTTAGTAGATTCTTTGAATAAATTTCATTTCATCTGCTTTTGGCAATAAAGTTGTATTTTGAAGTATGAATTCTAAGTTTTAAAGGATTTTTATTTTCTTTTTGAAATAGTAAATAGGTTGGATATTTTGATTTTTTGCTATTTGGATCTATTAATGAATTTTCTAAGTTTAGAAATCCCCCAATATTTATGTTTGAATATTGAGAGTTAATATTGCCATTATGATCAATATATCAACTGCTAATATCAGAACCCATATAACTAATAGGTTGGCCAAAACCCATTAAATCAATATATGCTTTTAATAACGCCTTTACTTGTTGGTTTAGTTTATAAATGTATTTTCCTTGCTTATTTCTAAACAAGAATCTATTATTAATATTTTTATAATTTTTTAATTCATCATCAAATACTCAATTGGCTGAATATATTACATATTTATTATAATAGTTGGCAATCATTTTCAATGTGTCATCACCAATTGATGATAGAAAAATATCATCATTTTTTAATTTATAATATAAGAAATTCAAAGAATCATCCAAAGTTAAATGACCGTCAAACATTTGAAAATTTTCATGTGCTGAATATGAAAGTTTTACAAGTTCTCTTGCAAAAAGTTCCTCAAATGAAAACAAGTATTTTATTTGATCAATATTAGTAGAATTTGCATAATAAACTGTGTTGTTTGTTGAATTATTAAAATAAAAGTTTTTATTGTTTAAAATATTGTATTTTTCAACTTCTTTGTTTGTTAAAGCGAGGTTAGCTAACTTGAATAAGTCATATTGACTAAATTTTGAATAAACTGGTATTTCTTTGTTTTTAGGATTAAATAATTCTTTATTGACTAAATATTCATCGTTTTGCATTGAACCATCGTAGTTTAGCGTTTTTCTAAATTCGGTTAAAAATTTGTTGTTGTTGCCATTGAATTTATTGATTATTTTGTTTAGTTCAGGAGTTGCTAATTTATAGCTTAAATCATTATTGGCAAATTCATCATTTTCCCTTATACTTCGGTTGTATGTATTATCAATGTGGTGTGCGTATTCATGAAATAAAGTATTAAAAACTAGCTCAACTCTCTTTTGTAAACTTCATGATTGGACTTGTTTTATTCCCCCAGAGATAAAATGATTTATTTGAATAAAAATGTCAAAAGCTTGTGGCAAATAAACACCATTTACATCTTTAATATTCGAAACATTAGGTATTTTTTTATTTATATAAACATGATTTAAACCATTAATTTCGGGACCATAAAATCCTCTTTTTAAAAACTCTAAATTCAATAATTTTCTTCCTTCTTCACCCAAAAAATAAATAGGTTCATTATTCTTTGTTAAAGCAAAAGGATATTCAAAAATACTTAGATTTCCATATTGATAATTCTTGTTCTTTAATTGAACATTGTTTTTGGTAATTGTTTTTGCATTGAAACTTTGTAGATATCTTTCTTCATTTGGCATTGAATAATTTTTATTTTTTTGGCCATCAAATAAACTCCCACCATTGCCATTTGAATAATTAACATAATTAAAAATGTAATATATTACGACGCCAATAATACCTGCCGTTAATATAAAAGCCAAGATTATAATAAATATTGCCCATTTTGGTAGAAATGCTTTTCTACCTTGTTTTTGCTTGCTAATTACTAAACTACACTCCTAACTAATTTTAATAACTAATATTTTATAATTTTAATATTATAATTAAATAATATGTTGAGAATAATAGCAGGAAAATATAGATCCAGAATTCTAAAGCAACCTTCCAAGTCAATAACAAGACCAACAATTGATAGAGCAAGGGAGGCAATATTTTCTTCAATTCAGTTTAACATTGAAAATAAGAAAGTTTTAGATTTATTTTCGGGATCGGGAGCATTTTGTTTAGAAGCTCTATCAAGGGGCGCAAAATTCGCGGTATGTGTTGATAAAAATGCTGAGGCTATTAAAATAATTAAAGAAAATCAAAATAGTTTAAAAGAACCAAATTTAAAAATTTTTAATATTGATGCATTGAATTTTTTAAATAATAATGTTGAAGTATTTGATTATATTTATTTAGATCCCCCATTTGTTGAAAAAAAATTACTAGTTGAATGTTTAAATAAAATTATTGATAAAAAATGTATTGATAAAAGTGGGACAATGATAATTGAAACAGATTGAGAAGACTTCAATTATAGCTATTGCGGTTTTGAAATAACTAAAAGCAAAAAATATGGTAAAATTTATTTCTACTATATTAGCAATATAGATGATATGGATTAATGAATATTAAACATTTTAAAATCTTATCAACTTAAGATTAGGAGTTTATATATGAGTAAAAAATTAATAATATTTACAGGACCAAGTGGCGTTGGAAAAGGAACAATTGAAAAGCCCTTATTTGAAGATAAAAGTCTAAAGCTAAAATTATCAGTATCAATGACAACGAGATCACCACGTGAAGGAGAAATTGATGGTGTACATTATTACTTTGTGTCAAAAGATACTTTTAACTCTTGCTTAGCAGAAAATAAGCTAATAGAATATTCAATGCACTTTGATAATTATTATGGAACATTATATTCAGAAATTGAACGTATTAGTGAACAAGGAAAAATTCCATTTCTTGAAATTGAAACTAATGGTGCTAAACAAATTTTAGAATATTATAAAAAACGTAACAGATTGGATGAAATTTGTTCAATATTTTTAATGCCTCCTTCATATGGAGAATTAAGAAAAAGAATTGAAGGTCGCAATACCGAAACTGAAGATCTAATTATTAAAAGAATGAAGAAAGCAAAAGAAGAAATAGGTTATTCTACAATATTTCATCATGTGATAGTTAATGATGATTTAAATGTAGCACTTGAAGAAATCAAAGAAATAATCAGATCAGAATTTTCAGATATTATAAACAAAAAAGAAGTTGCAAGTGAAGGTAGAAAGGCATAATTAATGGTAGACTTTTGCATAGCGTCAGATCAAGGAAAGTTTAGAAAAGAAAACCAAGATAGAGCCATTGTTGTTAATGGAGAGTATTGAACATTGGCTGCTTTGTGTGATGGTATGGGCGGACATGTTGGCGGTAGCCAATGTTCAACATTATCAACTAATAGTATTAAACAATATTTCCTAGACACATTTCCTCAAAAGTTGGAATGTAGTGATAAAAAAAACGTTAGCAAATGATTTAACAATGCAATAAGCTTTATAAAAGAAGCTTTAGTTAATTATGCAAAAGAGTATACTGAATTTGAAGATATGGGGACTACAATGGTTGTTGCCCTCATTTTTAATGCAAATGGCTTAGCTTATGTCTTTAATATTGGCGATTCACGCTTGTATGCATACAATGGATTACTTTACCAAATCACAGAAGATCAAAATTATTTATATCAATTGATGAGAGAATTTAATTTAACATACGAAGAAGCGGCATTAGATCCCAATTCTTACAAACTTATAAGTTGTTTAGGCCCCAATAAAAAAACCAATTGTCAGTCATTTTTTATATCACAAAAATCAGCAGTTAAATATTATTTATTAACATCCGATGGATTACACGATTATGTTTCTAAACCAATAATAGAAACTGTTTTGCAAACAAATAAGAGTTTAAAAGATAAGTTAAACCTTCTAATAAAATATGCCAAAAAAAATCTTTCAAAAGACAATATAACCGGAATTCTTGTGGGGTTGAAAAATGAATAAGATAAAAGAATTACCCGGCATGTTTGTACTTAATAAGTATTTTACTGATTTTGAATTAATTGGGACTGGAGGATTTGGCGAAGTTTATTCTGCTACATATAAAAAAAATAATAAAAGAGTTGCAATTAAAATACTAAATTGTATCGATGATAGCCGCTGGACAGAGCAGAGAACGAGATTCATTAACGAAATAAATATATTAAAGGAAATTCATAGTTCTAACGTAGTAAAGATATTAGGCTGACATTATAAAGGAAACGAAGTTTTTTATGTAATGGAGTTAATTAAAGGCCAAGGTTTAAAAACAATAATTGCAAAAAACAAAAAAATCGATCCTGATACTGCAATTATGTATGCAAAGCAAATATGTCAAGGTTTGATTGAAATCCATGCTGCCAAAATAGTTCATAGAGATTTGAAACCAAGCAATATATTAATTGATGAAGAAACAAATAGCCTAAAATTAATTGATTTTGGCATCTCAATAGGCGAATCAACTCAACGTGTAACTAGAAATTATAAAGCTGTTGGTTCTGTTCAATATATGGCACCTGAATTATTGCGTGGAATAGCTGATCCTTCTCCGAGAAGTGATATATATGCATTTGGTATAATTTTGTTTGAAATGCTTGAAGGCAAGGTTCCTTTTAATAATCATGATGATATTAACCAAATTATGATGATGCACATCAATAATGAATTGCCTAGAATGAGTGATGTCAATGTTATTATTCCACAAGCATTAGAAAATATTGTAATTCGTTGCACTGCAAAATCTGCAGAGCAAAGATATACTGATTGTGTTGAACTAATGCATGATTTAGAGACATGTTTAGATTCAAAAAGAGTAAATGAAAAAAGATTGGAATTAAATCCAAGTAAATCAAAAATGAAAGCCAAAAGAATCTTTAATAGTAAATTATTTAATATTATTTTTTTCACAACAGGGGGACTTTTAGTAGCCTTTATTGTGATATTTGTGGCTTTATTTTTAAAGGGGATATTGTAGATGATAGAAGGTAAAATAGTTAAATCAGTTGCTGGTTTTTATGATATAAAAAGTTTTGAAGATAAACAAATTTATCGTGTAAGAGGAGGAGGAAAACTTCGCTTGCTTGATATAGTGCCAATTGTGGGTGACAACGTTTTGTTTAAGAAAGACGAATTTATCTATCAAATTTTAGGTCGCAAGAATTTTTTTATTAGACCTAAAATTGCAAATGTTGATCAAGCAATTGTTGTGATGTCGTTGGTTGAACCTGAATTCAGTTCATTGCTCGTTGATAAATTTTTGATTATTATTGAAAGTAAGAATGTTAAGCCTATTATCGTATTAACAAAAAAAGATCTAACTTCTGATTCAAAAATAGCTTTTTATAAAGATCAAGGCTATGAAGTTTTTGAAATTAATTACGAAACAGGGCAAGGATTTGAAGGCTTAGGAGAAATATTTAAACATAAGACAAGCTTCTTTGTTGGTCAAACAGGAGTTGGAAAAACTACCTTGATAAATTATTTGGCAAAAACAAATTTTGAAACACAAGCAATTTCTAAAGCCTTAAACCGTGGAAAGCATACAACAAGAGAAGTTAGTTTGATTGATTTTAACGGTGGTGAAATAATTGATACACCAGGTTTTTCTTCAATAGAATTTGATTTAACAATTGAAGAGCTACCGATTGCTTTTAAATCGTTTAGAGAGGCTGCAAGCAAATGCAAATTTAGAACAAGTTGTAAACATTATTTAGAATCCGTTGAGGATTGCGAAGTTAAAAAATTAGTTTTACAAGGCAAAATTAAACAAGAAAGATATGATAATTACATTAGTTTTTTAAAAAGACTAACAAATCCAATAAAATAGGAGAAACTATGAAAAAAATAAGTCCATCAGTTTTAGATGTTGAAAAGAATAAATTAACAAACTATATTGACCAATTAGTTGATTGAGGTGTTTGCAATGTTCATTATGATGTAATGGATGGAATTTTTGTAAACAATACTGCTTTAATATTTCAAGAAATCAAGCAAGTTAAAGAAAATTGTAAGAAACATACAATGGATATTCACTTGATGGTTAAAGATGTTTTCAAATATTATGAAATGTACAAGAATATTGGAGATATATTGACATTTCATTATGAAGCATTTGAAAATAGTGATCTTGAAAAGATGATTAAACAATGCCAAAAAGATAACGTTAAACTTGGCTTAGCAATAAAACCTAATACACAAGTTGAAGAAATAAGACAATATTTAAAACATTGTTCTTTGGTTTTAATAATGAGTGTTGAACCAGGCTTTGGTGGCCAAAAATTTATTGAAAATTCATTTAATAAAATAAAGGAATTAGCAGACATTCGTAAGGCTTTAGATTTAAACTTTATTATTGAAGTGGATGGTGGAGTTAAAGACCAAAATATTGCCAAATGTTTTGAATGTGGCGTTAATTTAGCAGTAGTTGGTTCTTATTTAGTAAAAAACTTTTCAAAAGAAACAATTGACCAACTTTTAAAATAATTATAATGAAAAAAGCAAATAAAATATTACTAGCTACATTTGCAACTACAATTTTAACATTACCTTTTGCCGCAATTTCTTGTAAAGTCGATCAAGTTAAAGAAAAAGAAAAATTAATAAACAATTCTAGTCTGCTAAATAAAGAAAACAAAAAAATATTATTAAATGAATTAAACATATTAAAAACCCGTTTAGCAAATTCAAAACTTAATGAGAAACAAAAAAACGAATTTATTGAAATTCAACTATTTTTAAAATTAATAACACTTAAAGAACTAAAAATATCTAACGAATTATTAAAATATTATAAATTAATGGCCTTGCTTCAAGATAAGTCTGAAGTAAACAAATACGCAGATTTAATCAAAAAATATTCAAAAAAATTTTCTGATGTTTTAAAGGATGATAGTTCAATATTAAATGATATTTACAATCAAGTTGCAAATTTAATTCCTAGAATTAATTTACTTAAAAATTCTTATATTTTTTCATTAAATATAAATAATAAAAAATTCACTTTTTTGAATAATAATGGAATAGTATTCAATTTGGATTTTATATTGAATAACATTGAAAACAAGGAAAACAAATATTCAATTTATTACAATACAAAGAAATATGAACTAAATTTAGAAATAAAAAACATTGAATTTAATTCAAATATTAAAACTGCATTAGATTTTAAAAATCAAGATTTTTTAACAACTAATTTTTTGGATTATGATAAAGATTTAGTGTGCAATGAAATGTTAAAAAATGCAATGGTCTATAAAAATTATGAACTAGTTGAAGATTTTAATGAAAATGATAAGAACATTAAATTTTCAAAAATGTTTACTAGTCAAGATTTTGTAAATAATTATTTGGTTAATTGATTAACAAACGAACCAAATATATGCCAAATCGGTGAAGATAAATTTTTAATTTATTACCATTTACCTTTTTCTATAGAAAATAAAAAAATTGTTCAAAAGCCTTCATTGTATATTTCAAATGTTGTTGCTGATTCAACAATTATTAAAGCAATAGCAAGCAGGAACGTATATTTATTGAAAAACTTATTTTTGTTGCTGCCAAAAGAAAATTACAATGAAAATTTAAAAGTCTTAAATAACTTAATGAAATAAGCATTTGAAAATTAGACTAACAATTAAAATACTGGTCTAATTTAGCTTTGTTAAAAGTTTATAAAAAGTTTGAATTTAAAGTATTGAGATGTCCATTACTAAAAAAATCAAATTAAACAAGATAATTATTAAAAACGCTTATCTTGTTTAATTTGATTTTTAATTTTGTGTATTTAAATAAAAAATCCTAAGCAAAGGCAATGTCAAAAATATAGTAAACAAAGTAACTATATAATTAAAATGTATAAAAAAGTGCTAAAATTCAAATAAATTAATTAATATGATTAATAAAGAATAATTATTTTTTGCTTGCGCCTACAATTTTTAACTAACTTTTTAAATTGATTGTTAAGGCTCATAATTCTGAGCAAATTAGAAAGGAAAAAAATGTATAAGAAAACAAATAGAAAAATATTGATTTCTCTTGCCGTATTATGTGGAGGTTTAGCGGTGGCATCAATTGCTACAGTTTCTGCATATGCGGGTAAATCAAGACCAGAATCAAAAATAGAAAAAGAACATCTTTTGAATGACTTTAAAGAGGCATTAAATCAATTAAAACAATTGATTGCATCACCAGAAGCTAAAAATATTGAAAAATCAATGGAAATCAATATCTTAAATAATGCCAATGTTTCAGAAAATTCTTCAATTAAAGAAATTCAATCTAAAATCAATCAAGTTAAATATGCTATTGATTCATTAAACAAAAAAATCGCAGATCGAAATAAAAAGGAATTTGAAAAATTTGAACAAATCAAAAATGATCTTCAAACTTATATTAATAGAGATTTAAATGTTGTTATATACAATGAAATAACTAAAAAATCTAAAAATGAAATATTGAAATTGAATAGCATATCGCAAAGCTCAAAAGAACAAGAAATTATAGATGCAATAAGCAAATTGTATGATTTAAAGAAATGAATTTTAGCCCAAAAATTATTAATAGATAATTCAATAAATGAACAAAAAAGACAACTTAAGTCTTTGATTGAAATTGCTAATCAAATATTGTTATCAAACAAAATAGTTGAACGTGACTTAGAATTTAAACAAGAAATTTCTAAGGCTGAGGCATCATTAAGTCAAACAAACAAAACTGCATCTGAACTTTTTAGAGAAAGAATATTGCTTCACAAATTTATTAATGAAACTCTATTACTTGAAGAAACAATGTTTAAAGAAAAGGAAACAAAAATTAACGAGCTTAAGAAACAATCAGAAACTATTTTTGAACATATTGGAAAAGACCCAAATAATAGCATATTCAATTATAATTCCCAATTTTATAGAAACATAATAACTAAAATTGATAGTTTGAACACTAATGAAAAAAGTTTAACCTTAAATAAAAAGATTGAAGATATAAGTAATGAAATAAATAATCTAGACAAATCATTTTCTAAGATGGTTTCTGATACCCTTGAAAAATTTAAATATGAGGATGATCTTAAAGACCATATTGATGTTGCAAAAGATCCCAAAAATTGAATTAATATTCAAAAATATATCTGATACATTAGGACAATAAGCCAAGTTAAAAAACTAAACTTAAAATCGTTAGTAGATTTTTATAGCAATGATCAATTTGCTAAATTTATTTGCAATACAATTTCAGACTTTTTTGATTATACAAGAGCAAAGAAAGATTTTGATTTTACAGATTCTGTTATTGCAAATTATGTACTTAATAAAGTTATTAAGATTAGGGATTTTGAAGAATTTAAAAACTTAAATAGTTATTTTGCAGATGAAAAAAATGATATTTGAGCAATGCAAGTAGACCAAATAACAGATGAAATGAAAACAAAAATCTTTGATTTGTTAATTAAAGATTTATCTAATATTTTAAAGAAAGATAGTTCTGAAAGCAAACTATTAAGTGCAATATTGTTGCCTACAATATTTAAAAAGACATATGAAAATCTTTCTAAAATAATAAAGCCAATTATAGATATGATATATAATAAAGACGCATATAACATAGAAATTATTGGATGAGAAAAAGCTTCACAAGAATTAAGTCTGGATATATCTAATATAAAAGAAGCAGAAAAAAAGGCTTTAGAAATAAAAGCAAAATTGACCCAAGAAAATTTAAAAAATAAATTAAAAGAATTTAAATTAGTAAAAGAAAACTTGGGAAATTTAATTAAAAAATTATCCGAAAAAGGCATCGAAGTCGAAGAAGATACAAAATTCATATATGAAAATTCTGAAGAAGACGAAAATTCTACATTATATGAAGTAATAAATTCTATTATTAGAATAAAAAAAGAAATAAGATACCTTGAATATAAACTAGATTAATAATCACTAAAATATAATAAAAAAATCCTTTTGATAGCATTAAACCTGGACAAAAAATTAGGCTCGTAATGAAAAAAACGGGTCTAATTTTTTGTCCTAGTTTAGTACTTATCTTGCTTTTTTTATTTTTTTAGATATTAGAAAGCAAAAATTAAACCAATTTTTGCTATATATTTATTATTTTTTAAGAATTTTTTGACTTTTAAAGGTAAATCTTAAATTTATATTTCCTTTACAATTTTTATATCAATACCAATATCATTTCAATTTCAAGGCGTATTGTTCTTATTTCCTTTATAGTAAACTTCTTTTAAAGAAGGAAAGAAGGGGTTTCAGTCATAATATTTAAATCCAGCTAAAAACGCATTAGTTCCAATTTCTTTAACTGAATTTGGAATAGTTACTACTTCTAGATTTCTGTTTTTAAAAGCATTTTTACCAATTCTTTCAATTCCTTCATTTAAAACTACTTCTTTTAGATTTTTAAAATCCTTAAATGCGCCTTCTTCAATTTCTTTAACTGAAGCTGGAATAGTTATTGATTTTTGATATTGAAGAACCCCTGTTATATTTCTATTAATTTTTTCAAAACCATTACGTAAAGTAATCTCACAGTCTTTATTAAATATATCTTTAGAAGATAGTTGAGTTACTGAACTAGGAATAGTAACAGAAGAAATTTTAGTGCCTAAGAATGCTTGGCTTCCAATACTATTAAGTCCTTCATTTAAAGTTAATTCTTTTAAATTATCGCAATAATAAAATGCATTAGCATAAACATCTTTTAAACTATTAGGAAAAATAATAGTAGTAACTCCTGTGCCTTCAAAAGCACCTTCTCCAATACTATTAAGTCCTTCATTTAAAGTTAATTCTTTTAAATTATCGCAATAATAAAAAGCATTAGCATAAACATCTTTTAAACTATTAGGAAAAATAATACTAGTAACTCCCGCGTCTTCAAAAGCGTTTTCACCAATTCTTTCAATTCCATTGCTAAAATCAATCTTCTTTAAATATCAACAATCCTTAAAGGTTTTTGCCTTAATTTCTTTAATTGAACTAGGAAGAATAGAAATTGAAGAGATTTGTGTGCCTTCAAATGCACCTTCTCCAATATTTTCAATACCATTTTTTAAAGTTAATTCCTTTAGATTTTTAAAGTTTTTAAAGGCTTCATTTTCTATTGATTTTACATTTTCAGGAATAGTAACAGAGGCAATTTTTTTAAGCCCCTTTGTTAAATCTTTAGTAATTTTGTCAAACCCATTATCAAATTTTATTTTGCAATTTTCAAAAGAAGCATTTTCATCTATTTCCTCTACCTTTGTGCCTTTAAAATAACTAGGTATAACAACATCGATTTGTTTGCCATTATAATTGGTAATTTTTCAATTATTTGAAGAATTTCTTTCAAAATCAAAAAGTTCATATAATTCTTTTTCTTTATTATCTTGAACATTTTGATTTTCGTTATTTGCAGAAGAATCTTCATTCTTTAACGATTCTGGCTTTTTTTCATTAGGTTTGTTGTCATTATTCTTATCTATATTTTGATTGTTTGAATTGCTGTCTGTTGTTTTAGTACATGATGCCGCCACTAATGGAAAGCAGATTGTTGAAGTTGTAACAAGTCCTAAAGTTAATAACATTTTTGTTCATTTTTTCATATTTAGTTCTCCTTATTTTCTTCTTCATCTTTAAAAATTGATTTTGAATCATATTTGTATATATCAAAAATTATTTTTAATATTTGTTTTGTTCTAGCCAGGATTTTTGGATAATATTCTTCTAAATTTCTTCGATTTTTATTTGTATTTTTTTCGTCTTTGTCATTAAAATCAACTCAACTTTCTAAATTAATTAAATAAGAACCTTTGCCCGAGAAAGAAATATTGCTTTCTAAATTTAACTTTTTATATTCCTTAAATTTATCTATTAAGTTTTTGTTTGAAGCCTTCTTATTAATTTTTTCATGAATTTGAAAACCATTGCCTATCATATCAATATATTTTTTTGCCTCATCTTTTGATTCTCCTCTCAACGCATTTATGCCGGCATCATTTTGCGCTATTATGTGCTCATATTCATATTTTGAGTCAATAAATATAGAATATATTGAATCAAAATTTAAATTAATTTGTTTTTCTCCGATGGATAATCTATTTTGATTGTATTCTCCGCAAGATAAAAGGAAATGAATTCTACTTAAAAGCAATGATTTCATTTTTGGTTTCATTAATTTAGATTTAGGATATTTTATGCATTCATTTAATTTATCATTAATTTTTTTGTCAATATCAGAAGGATTATTTTTGAATCTTTTAAGTTCAGGAACAAGGTCAATTAATTGTTCTCTTAACTCATCAGGATTCTCAATTTCATCATTTTTTTTAATTTCATCAATAATTCGCGAAATTGATTTTGTTAATGATTGGCCTTCAAATGCAATCGTTTTTCAAACAAAATTAAATCTCTCTATTTCGCGTAATAAATTAAATGTATTATTTAAATCAAGATCTCTCATTGGTTTTTCATTATGTCAAATATTTAACTTATTAAATATTACTCATACTAATGGTATAAAAATTGTATTTTCTTGAATAGCAGCATAAACTTGCTCAGCAAATGCTGAACGTGAAAAAATCTCATTTTGAATTAATGCTTTGGCATATTTTGAGTCTTTTTTCTTCTTAGCTTTATTATTCAAGTTATAGCTATTAAAGAAATCTTTAGGATTTTTTAAATATTTATACATATACAAATAATTAATTAATTTGTTAAACGTTTGTTTTAAATTTTTAGAATTATTATTTAATTCATTATTAAATCATTTTTCAAGTAGTTGAAAATTATTCACATTCTTGTTTAAATTTTTTTCATTATCCATTTGATATTGAGAATCAACTTTGTTTATTACGTATGCAAAGTTTTCAACTTCACCTTCCTTGATTTGGTTTTTTGTATTGCTTAATGCTTCATAAAATTCATCATTAAATATTTTTATGTTTTCCTCGTTATTTGGATTCTTTGGATCCAATGAATATAAGTAATTTTTAAATAAATCTAGCGAATTTAAAGGTTTTGAATTAAGGTTAAGATTTTTAAAAAGAATTTCGGGCTTGTTGGTTTTAACAATTGTATATGTGATTATAGATTTGTTTAAAACATAATCTATTTTATTAGTAAGCCCCTTAGTTTCTAAGTTTTCAAGTTTGTTAAAAATAATTATAAGTTTTTTAACAAAATCATTATTTTTGTCATTGGTTTCAATTTTTAAATTATTTTTTATAGTTTTATTTATAAACGAATAAATGTTGTTTGATTCAAAATTTTCATCAATATTGCTATAAATACTCAAGTATTTTCTTTCTATAAATAAATTGAATAATAGCAGTCAATTTTTATCATTTATTTTAATTGGTTCTTCTTTAGATAAGAATTGATGGCAATAAAGTTTAAATAAACAAAACAAAATCAATAGAATTGTAAAAGTTCGTTGTTGGCCATCAACAATTACAATATTTGTCTTTGCAGATATACAATCTTGTTTTGTAACAATAATATTATTCAAATAAGAATATTCTTTTTCATCGTTATTAATTAAACAATCAACAAGGTTTTCTATTAATTCCTTGTTTCAAACATATTTTCTTTGAAAAATAGGAATTATTACATTTTCGAAATAATCATTTAACAAACTAAATATTGTTAAATGAATACTATAAATTTCATCATTTAATTTATTATTTCCTTGACATTTTGAATTTTGGTAATCTTCTTGAGAATTATTTTCAATAGAAAAATTATTTTTTAAAATTTCTTCATTAAGGATTGTCGTAATTCTTTCATTGGGCAAATCTTTTATCCTTTTAATTCAATTAACAATCGAATAGAAAGAATTTTTTATTGAATTTAACAAATCATCTTTGCTATATTCTGAAGCAATTTCTTCTAATATAGGTCCAAAATAGTCGTCCTTGTTATCTTTACTATTTCTTATAGATTTAAATTCGGATTTAATTAGCTTAATATCTTTGTTAATTTGTTCTTCATCTTTTTTGTTTTTTTTGAATAATTTGTTTAATTTGACTAAAAAAGAAAACGAAATATTAGAATCTTCAACACAATTTATTCCTTTAATTTGGTTTTCGCGAAGAGATTCATTAACATTTTTATCATAAGTTTTAATGATTTCGGTAAGAGTTTCAAATTTATTATTAATTTGGTTTAATAAAAAATCATTTAAGAATAGCTTTACATTATTTTTATTTGAATTATTTTTTTGAACAACACATTCAATAAAATCGGGTGTTAATTCGTATTCATTTTTGTTTATTTTAATTAATATATTAAAATAGATTGCAACTTTCAAAAATTGCTCAATAGTGCTATTTGATTTATTTTTATAAGATGCATTTTTACTATCAAAGTCAAAAAATAATGAATATTGTTTATTTGGTTCATTATTGTCAATTTGCTTTCAAATTAATTTGTTATTTTTTTTAATTTTAGAATAAAAATTATCAATTTCTAATCAATCAACAATTTTGTCATAATAACTTTTTAAACTAGTTCCTATTGTTCAAGTTTTCAATTCTAATTTACTCATATTTTTCCTCTCATTTAGCCGAAATTTCTATATTCAGATGTTTAAATATTTGATTTAAAATATCCGCAACTAATTGAGTTGGTATTGAACTTCTTAATGAATATGACCCTCTTGCCATATGGTCATTATTGCCTTTTTGCCTCTTCTTTAACAAATCAAGTTTTATATTGGATTTAAAAATAGTAGGTTTTAAACTAAACCTATTGTCGTATGAAGAATAATATGTACTATTTTCATTGCCTTCAAAGTTTCAATGATTTCTTTGATATTCCCAACTTTTTGACGTTTTAGGGTTTTCTATCACTCACACTTTTGGCTTGAATATTTGAATTATTAAAATAGTTCCGGATATAGTACCTTCTCCAATTAATCTACCTCGTTCTTTTTGCTCAAAGAATCTATGTTTTACAGGATGACAAGTCTTATTGTATTCATCATAATATTTTTTGTTCATGACCACTCAATGGCAGTCATTTGAAATCGAACGCAGCATTTTGCCGTTACAATCTGCTCCTGATCAACTTTCACATGGCGGTGAGGCTAAAATAATATCTGGTTTGGGCAATTTTTTTAGTTCATTAATTAAATTAAAATTTTGAATTGAAAGGTCAATTTTGTGATAAAAAAACCTCTCTTTGTCTTTAAAAGAGATGTCATTTATTCCAATTGAATGCACTTCTATATTATTAAAATTCTTATAGTATTTTTTAACAGCATTTTTGTATGATGACTCCGCATCATCAAAAAGAGCTCAAATAATTATTTTACTTTTTGGCGAAGAGCTCTCTCTCTCTCTCTCTCTCCATACTATTACTATTCATATATATTTCCCCAATCTATGTATGAACTACTTCTAATTACTTTATTTTAATAATTATACCTATTTTTAGTGTCTTTCTAGTGCTATTTATGTTTAAAAATATTGTTAATTTTTATTAGTCATAAAAAATATAAACAATTTTTTGCTTTGTTTTTTATATCTAAAAACATACTTTATTAAATAAAGATATATGAAAATAAAAAAATAAAAAATTTTTAGCACTCTTATGCTTTTAGTGCTAAAATATTAAATGTAAATTGAAAAAATTCAAATTAAAAAATATATTTTAAAAGGAGATTAATTATGGCTAAAGAATTTATCTTAGGTATAGACTTAGGTACTACTAACTCGGTAGTATCTGTTATAGAAAATGGAACCCCAAAAATATTAGAAAATCCAAATGGTAAAAGAACTACACCATCTGTTGTTGCTTTTAAAAATGGAGAAACAATTATTGGTGAAAGTGCTAAAAGACAATTGGAATCAAATAAAGATAGTGTTGCATCTATAAAAAGATTAATGGGTACTTCTCAAACAGTACATCTAAATAATAAAGATTACAAACCTGAAGAAATCAGCGCAATGATTTTAAGTTACATGAAAGATTATGCTGACAAAAAATTAGGTCAACCTGTTAAAAAAGCAGTTATCACAGTTCCAGCATATTTTGACAATGCACAACGTGAAGCAACAAAGAATGCGGGAATTATTGCTGGTCTTGATGTTGTAAGAATTATTAACGAACCCACTGCTGCCGCATTGGCATTTGGATTGAATAAAGATAAAAATGAAAATCAAAAGATCTTAGTATTTGACTTAGGTGGTGGTACATTTGACGTTTCATTGCTTGAAATGGAAAGTGGAACATTTGAAGTTTTGGCAACAGCAGGCGATAACCACTTAGGTGGTGATGACTGAGACCATGAAATTGTTAAATGAATGGTTGAACAAATTAAATCTAAATACAATTTCGATCCAACAACCGACAAAATGGCAATGGCAAGATTAAAAGAAGAAGCAGAACGTGCAAAAATTACTTTATCAGAACAATTAATTGCAAACATTTCTCTTCCATTCTTAGCAATGAATGAAAATGGACCAGTTAATGTTGAATTAGAAATTACTCGTGCTACATTTGAATCAATGACTGAACATTTACTACAAAGAACAAGAAAACCATTATTAGATGTTTTAAGTGAAGCAAAATTAACATGAAATGATATTAATGAAGTGTTACTAGTTGGTGGTTCAACAAGAATGCCAGCTGTTCAAAAATTAGTAGCAGAAGTAACTAATAAAAAACCTAATAATTCAATTAACCCTGATGAAGTAGTTAGCGTTGGAGCTGCTATTCAAGGTGCAATATTAGCAGGAGAAATTCAAGATGTTTTACTACTTGATGTTACTCCTTTAACATTAGGCATTGTAGTAGAAGGTGATGTTGTAGCGCCTTTAATTCCAAGAAATACAACAATTCCTGTTACAAAGAGTCAAATATTCTCAACAGCTGTTGATAATCAAACAGCAGTAACAATCGTTATCACACAAGGTGAAAGACAATTGGCTAGAGATAACAAGATTTTGGGTCAATTCAATCTTGAAGGAATTGAACCTGCGCCAAGAGGAATTCCTCAAATTGAAGTAAGTTTCTCAATTGACGTAAATGGTATTACTAAAGTAACAGCAAAAGACAAAAAGACAAACAAAGAACAAACAATAACCATTCAAAACACCTCTTCATTATCAAAAGAAGAAGTAGAAAAGATGGTTAAGGACGCAGAAGCAAATAGAGAAGCTGATCAAAAGAAACGTCACGAAATCGAAGTTATTGTAAAAGCAGAACAATTAAGCAACGACTTAGAAAAGACATTAAAATCAGAACAAGCTAAAAACCTAGGCGAACCTCAAAAACAAGAACTTCAAAAAGAAATAGATGAAATTAAAGAACTAATAAATAAAAAAGATATCGAACAACTAGAAAAGAAAATTACAGAATTTGAACAAAAAATGGCTCAAGCTGCTGAATTTTTAAAGAAACAACAAGGAAATAATAATCCAAACACCAACAACGACAACCCTCAAACTAACTAAAAATAATAAATTTAAAATAACGGTTTTAAGCCGTTATTTTTTTGTTTTTTGTCTTTTAAAAAAAATCTTCAAAATAAAAAGCAAAAATGGGACTAAATATTGCATTTTTCCCAAAATAACACTTTTTTTATAACTTGATTAATATTTTTTTTGTAAAATTTTTAACATAATTGAAAATTTTAATAGAAGTTAAAGTTTAAAATAAATGGAGGAAAATAAAAATGAAGACAGCTTTAAACAATTATCACAATTTTTTATTTAAAGTTACTGCTAAGAAAAAAAGCACAATAATTATTCCTATAATTTTATTGTTATGCAGTTTGATTTTGTGTTTTGTTTTTGTTGGTACTAAACCAGCACCGCGTTATTTTAATGTAATTATTTTTGCATATACATTAGTTGCAATATTATTTACAGTATTATATGGCTCTCTCAAGTCCTTAAATATATTTAAAGATCTTGAACAAGATGGGATTGAATTAATTATATTTTCTAAGCCCATTTCAAGAAAAGCAATAATCTGAGGGAAAATTTTAAGTTTTAATTCTCTTGGACTTATTTGAACATTGTTTGCTTTTGTTAGTTCTATTATTGTATATAGTCAAGTTTCAAAAGGAAACATGTTTGGATATTTAGTTTTGTTGTCATTGGTTGCGCATTTTCTTGCATATACAATTTTTGGATATATAGCAGCATTGATTGCATATAAAGTTAACCAAAAAATTGCTATAACTGTTCCAATCATAATTTTTGCACCTATGGCAATAGGTGGAGGATTTATATTCGCCAACTCAACATCAACAAATGAAAATTTTGCTCATTATATTAATAGCAAATATAAATACCACAGAGCAGGAAACGAAGTTAATAGTGAAGTATTTTATTTAAATAAAAATGATGATAAATATTATTTAGTTCCAAATGGAATTAATAATAACAAGTTTAGTGATGTACAAAATCAATATTTAAATTTAGCCTGAAAATATTCAAATAGTTCAGCTAATGAATGGCAAAAATATTCATGACTTGCAATGCCATATCAATTTGTTGATATATTTAACATTGAAAATCAAAATATTTTTAGCAACCTTTCTTCAGACAGTATTAATAATAGTTTAAGTAATTATTTATATTATAAAAATCAAGAAAATACAATATATAAATATAAATTAAATAGAAATTCGGACTTATTAAAATTACCAACATTAGAAAATAAAATTAAGATTAATAAATACTATGTCCCAGGAATGTTAAATTTAAATTCAAATATTGATAATGAAGTAAATAATGGAGTAATTTACGCTAGAAAAAATGCTTCTAATTTTTCTGCCTCTTTTGAAGAAGACAACTTTGTTTATGCATCTCCAAATAATCTAGTAGGAAAATTAAAATGAAAAATAATGAGCCAAGTTTTAAAATATCATAAATTTACAGAAGCAGCCGAAGAGTTTTATTCTAAGGTTAAAAATGAAATTAGTTTAAAGAAATTAGTTAAACCATATGATATTAAAAAGCTATTGTTAGAAACGATTTCAAATGAAGTTAATAATACAAATAGTAAGTTCTTTAATATAGATAATGTTAACTTAGCAGTATTCGACCCTCATGGTGTTAGAGATGGAAGATTACAATCTGAAACAGAAAGAAAAATTTATATTGCCACAGCGTTGCTATATTTTATCTATTTTAAAGATAATCAAGGTCAAGTTATTAAATCATTATTGAAATCTGAAAGTGATAAAACAACATATGCACCAAGCCAAATAGAAATCAAGGTAGATGGTTATATCTATGAAATAGGTGGATATGAAAGCTACGAACCAAAACAACAAGTTCAAGAAAATAAAATAACTAAAAATAAACAAGTAGTAGTTAGATATGAATTAATTCCAAGTAATAAAAATTATTTATTCCAAACAGTAGATGATGTATATTCAATTGAAAGAGATAGAAAAATAGTTAATAAACAATTTTATATTTTAATTTGAGTAGTAGTAGCAATAATCTTAATGATGGCAAATAGATTTCTATATCTAAGAAAGGATTATAAATAATGGATAATCATATTTTAGAATTAAAAAATGTAGTAAAAATCTATAATAAATCAGATCGTGGAATTCATAATATTTCATTTGCTGTCGAATCTGGAAATTTTCATGCATTTATTGGCGAAAATGGTGCGGGAAAAACAACAACAATTAAAACAATAATTGGTTCTTATATAAATTTTAAAGGTGAAATACTTATTGGCGGAATTACAAACTTAAACCCTAATTCTAAAAAAATTCTTGGTTATGTTCCAGAAAATGCAGTGTTTCCTAAAGAACTAACAACATATGAATATTTAAAGGCCCTTGCATTATTATCAGGAATAAATAAAACCGAAGTAAAGGCAAAAATCGAAAGTTATTTACAAAGATTTGGAATTATTGAATTAAAAAACGAAAAGCCCGCTAATTTTTCATCAGGTCAAAAGAAAAAAGTTTTATTAATACAAGCTTTAATTAACGAGCCTAAATTATTGATTTTAGATGAACCAGCTGCTAATTTAGACCCTACTGCAAGATTTGAATTGTTTTCAATATTACATGAACTAAATGAAAAAGGAACAACAATTTTTATTAGTTCCCACGTTCTAAGTGAAATTGATAAATATGCAAATTCATTAACATTAATTCATGGCGGAAAGATCTTATATTCTGGCCCTAAATATGATTCATTGGAAAAAATATTTTATGAAAAAGTTATTAAAAATTAGTTTGTCATTGCCGTTAGTTATTGCAACAATTCCATTAGTTGCTTCAAGCTGCAAACAAACAAATATTGAAATTAAAAAACCAGATGATAAAAAAGAAACAGCAAATCAAATCCAAGCAAAAATTAATAAAGAATTTCAAAAAAATAAAGTGGTTCAAAAATTACTAAGTTCATTTATTGAAAATGAAAAAGAACAAGATATTTTTGTACAAAATCAAAACAATATATTGCCCAATGTATATTCAGAACTAAAGTTTTCTTTGTTGTATTTCCCTATTTATAATAGAGCCTCAGATAATTCGGGTAGCGAACTTTATGGATTTATAAGACAATCAAGAGAAATTATTGAAAACACATTGCAAAGAAATTGATATTGATATTTAAAACAAATTGGTAATTTTGTATATTCAATAAACCCTTATAATGATAGATACATTGAAACCCCTCAAACAAAAAAAACAATTCAATCTGCCCAAAAAGACATTGGTATTTTATATAAAATAAAAGATGCAATGCCTAAGGATATTATTGAATTGCCTTATAACCCAATAAGTTCAGATGAATTAAAAGAAATAGTTGCTAAAAAAATTAATGAAAGTATTGATTCTAAAGTTCAAGACCAATTTAGCGATTATTTAGAAGATATAATTAATGATTCATCAAAGTTAAAAATAGATACAAATTCAGAAATAACCAATTTTTTAAGAAACAAAATTAACTTAAATGATGCAAAAAAGCTAATAGAAAATTATTTGAACAATAAGAACAAATTTGAAACAAGTAATGAATATCAAATTAATAACGAAATTTCTAATAAAATATTTAATCTTTTAAATACAAAAGGATCTTCATTTGATAACTATTCCTCAAAGAAAATCTTTTACGTAGCATACGACCACAAAACTTGAATAAGAGTAATGGTTTATACTGAAAATAACGTTAAAAAAATTTCTATAAATCCTGATGTTTATATTTTAAATCAAAGCAATAGCTATGAACAAAGTAAAGAAATTTTAAAAAAATTAAATAAACAAATTTTTATTGAACGGTTGAATTCAATTATTGATTCTTTAATTGAAAATATAAATGAAAAAGATTTCAATATTAAAAACTTTTTTGAAAAAAATAATGATAAAAAATTCTTTGAATTGTTTCAAAAGTTTTCATACAATAAATACTTGATTGAAGCAATGCAAAAAATTAATAAAGAAAAAGATCTAATTTATCGCTATAGTTGGGAGACAGTAGATTATGAATAAAAGAAATTTATTAAATTTTTTGTTTGTTCCCACTTTAATTCTTGCACCATTAACCGCTATATCGTGCGTTGATAAAAGTGAAAAAAGCGATTTGAATGCATTAGCAACTAAAATAGGGGATGTTGTAAATAAAAAAGAAAATCCTGCTATTGAAGTAACCAAAAAAATAATTAATGACTTATTGAAACAAGTATATAGGGATAATGATGTTGAAAAAATTAATTTTTTGGATCAACAACAAAATAAAGAATATCAAAAAAACATTTTTTTGAAAATTAAAGAACTTTCAAAAAAATGAAATTCTAAATTAAACAATACAGAAAAAGAAAACATTGCCAATCAATTAAATCAAATTTATGAAAAAAATTGATATATTGTGTTAACAAATATAAATAAATTTAATTTAAGATTCTATGAATGACTTTTGTTTGATAAGCCTGAATTTTTAAGCCAAATTAGGGCAGATGGAAAGCATAGCAAAGAATATTTAAATTCATTAAAAAGCGAAAAAAAACCAGATAATGTTCCGATTATTAATAATTATTTAAGGGAATTAAAAGAAGGCGATGAATCAGGAGAACTTTCAAATATGAACATGCTTTATTTAAAAAAAGATAGTATGATATTCAGATTTAGAGTTGTTGATACATTGACAAAACCAAAAATCAAACTAACTTGTTTTGTTTGATATTTTAGTAAATTTAAAAATATATCAATAAATTTGATTTCAGATATTGTTCATAGCGCTTATATTCACGGCGTTCAAGAAGGATTCAATAGATTTGAAAATGATATGGCAAACCGTTATGGCAAACCTGCCAAAATGATCATGGGAATTTTCGATGATAATGCTTCTAGCGCCAACGGAGGGGAAGAAAATGAATAAAAAAATTTTGATGACTCTATCATTAAGCACGTTAAGCATTATACCTAGCTGTTTTTCTTTAATTTCATGTACTAAAGAAAATAAACAAGATGAAAAAAAATCATTGAATCAAATAATTGAGGGCAACGAAAATGCAAACGAAAGAGAATGAAAAAATTTTTTAAATAGAGATTATGTTAAAGAAATTTTAAGACTTTCGTTTGATGCTGATGTTGATGCTAAAAACAAATATATAGCCGAACAAAAAAAATTAAATAATAATATAGAATATTCAAAAAAATTAAAAGAAGCATTGATATATGGAAATTCTTTGAAGGCTTCTTCAAGTTACAATAGCGATGATTTTTCTTCACAACCTAAATCATTGTTTCAAAACGGAAAAAGGATTTTTAATGAACTTAAAAGTAAGAATTGACTTTGATTTCTTTACAACCTTCCAAAAATAATTTTTGGTTATTTTCCTCCAATGAATAAATTTGAATCATCAGATGAACAATCGGCGTTAACCGCGCAAGGTAATTCCTTGATTTTAGGCCATTTTTATAAACCAAAGAGTAATGAAATTATTCAATATACAACTCAAGTTACAAAAAATGACAAAAATGGTGAGAGAACTTATGATGTTTATTTATTAACAAAAGAAGGTTTTATTCTTCAATTAACTATTGATTGAGCAAAAGATGATGAAGATAATAAAATAAAGCCTAGTGTTAAACTTTTTGGCTATATTTACACCTACCCAAACATTGTAAAAGATAGAAATAATTTAAATATTTTCAATATTCAAAAATATGTTATTGCCAATACGGAATCGTTTGATGATATTAACAATAGAACATTGCAAATATTATTTCAAGATCAATATGGGGGCAGTCCCTTACGTTATACAATAATTGATGTTAGTTTAAAATAAAATATATTGTCAAGATAAAAATTCAAGTGTACACTAATTTGAAGCAAAATTAAGCGTTGCCTTGATTTTTTTAAATTAAAATTTCAATAGATATGAGCAATAAATCAACATATATCTCATTTTCTTTTGTTTTAAAAATAAAGTTTTTTTGCATATTGTAAATTGTAAATTTTAGAATTTAAACAATTAATTTTCATATTTTTGATACAATTTTATTGTATTTATTACAACCGGTAAATTATTCAATTATATTAATTTATCAAACACTATTACGGATTCATTTAACTAGTGTTAGTAAAGATTACTAATGTTAAATGTTAGAAATAATAGGAAGAACAACAAAAAAACAAAGGAAAATTATGTCAGAACAAAAACAACAAGCACAACCAGCAGTAGCTCCAACACAAGCAGAACCTGCTATTGTATCACGCGAAAAATTATTAGAAGCAGGAACATACTTTGGACACAGAGTAAGCCAATGAAATCCAAAAATGAAACAATACATTTGAGGAAAAAGAATGGGAATTCATATTATTGATATTGCCAAAACTCAAAAAACATTGGAATATGCTTACAAACTATTAAATAAAATGGCTCAAAAACCAATTTCATTTATTTGAGTAGGAACAAAAAAACAAGCTAAAAAGGCTATAGAATCAGCCGCAGCAAGAACAAATTCAGTTTATGTTTCAGAACGTTGATTAGGTGGAACATTAACTAATTCACAAACAATTTTTAGAAGTGTTAGAGAATTAGAAAGATTAGAAGAACTACAAAAATCAGAATATCAAGGATATACTAAAAAAGAAGGTCTTCTAATGGACAAAAAAATTGCCAAGTTGCAAAAGAACTTAGGCGGTATTAGAAAATTAGCAGGAAAAGCAGCAATGCCACAAGTAATGATTTGTGCATCACCTATTGATGATGAAATAGCAATTAGAGAAGCAAAAAAGAAAGGTTTGAAAATTTTTGCAATTCAAGATACAAACTCAAACCCAGATTTAGTTGACTTCGTAATACCAGCAAACGATGATTCTGTAAAGTCAATTACTTTAATTACAACTATCTTGGCAGACGCAATAGCATCAGCACATGGTGAAAAACCAGTATATGCATATAAGTCAAATGAAGAAATTATCTTGCCAGAAGAACAAAAACCAGAAAAGGTTGAAAAAATAGCAAACACTGAAGAAAGAAAACCAAGAACTTTTGTTAAAAAAGCTAACGTTGAATCAGAAAATACTAAGAAATCTGAATAAAATAATTGAAAGGGAATGTTATGTCAGTAGATTTAAAAAAAATTAAGGAATTAAGAGAAAGAACAAATTCAGGATTTTTAGATGTTAAAAATGCATTAGAAGCAACAAACAACGATATTGAAAAAGCAATTGAATGATTGCAAGAAAAAGGTATAGTTAAGGCTGCTAAAAAAGCCGGAAGAATTGCAGCAGAAGGAATAGTAAGAGCTGTTATTAAAGATAATATTGCTGTAATTTTTGAATTAAATTCAGAAACAGATTTTGTTGCTAAAAATCAATTATTTTTAGACTTTGCTAAATTATTAGAAGAAGAATTGCCAAAAGTTAATTTCTCAAATATCGATGATTTATTAGAACAAAAAATTAATGGTAAAACAATTGAAGAATATACAACAGATTTAACAGCAAAAATTGGCGAAAAGATTCATTTAAGAAGAGCATTAAAGTATGAAGCAAAAGAAGATGAAGTTGTTGCTGGATATACACATGCTAACAATAGAATTGCTACTATTTTAATCGCTAAAGGACATAACCAAGAAGCATTAAGACATGTTGCAATGCATATTGCAGCCTTAAACCCTGCTCATTTATTCGAAACATGTTTGGATGAAGAAGAATTAAAAGAAATTTATGAACGTATTAACAACGATCCTAAATTAGCATCTAAACCAGAAAAAATTCAAGTTTCTATGAAATCAGGAATGTTAAGAAAAGAATTCAATGAACGTGGCGTTTTAATGTATCAACCATTTGTTATGGAAGAATCAAAAACAGTTCAACAATATTTAACAGAAAACAAATTGCAATTAATAGATGCAACAAGATTTGAAGTTGGCGAAGGAATAGAAAAGAAAACTGTTGATTTTGCTACAGAAGTTGCTGAACAAATGTCACTTAAATAATAATTAAGTGTTAAAAAAATAGGCTTTTGCCTATTTGCCAAAATGAAAATTCAAGTGCAACACAAATTTGAAATAAAATTTAGTTGTGGGGCACTTGAATTTTTTTAAAATAAAAAGCTCCACAAACGTACCAAGGGAGCCTTTATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGTTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCGAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAGTTTTCCAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGAATAAACACAAATAAATGAAAAATAAGAAAATCCGATAAATTAAGGCAATATTATAAAAAAGGAGGCAAAAGAACTGCTTCGGTTCTTAGTCGTCTAATAAAAAGTGCAAATTATGTGTTTCCAATATGAACAAGACCAAAAAATATTGATTTAAGGTTGGAATACGGGCATTGAGAGGCAGATTTGGTTCTTGGAAAAAGAGCAAATGGCTATAAAAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGCGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGAAATGGCATGATAAGAAAAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGAAAATCTGCATATGAATTGTTTATGTATGAAAATTTTGGCGAAATAATAAACTAGAAAAATTTAAAAAATATAACATGTTGTTTATCACAACGTTTTTTCTATGCAAAAATTTTAAAAATCGCATAGAAATTTTAATAACTTAATATAATAAAAACATCAAGCACAAAAGTGTTGCACTTGATGTTTCATTCTGGGAAATAGGCTTTTGCCTATTTTTTTTGATACTTTTTAGTTATTTTTTTTAGCTTAATTAAGAAATGATTCAATGCAGAACGAGACTTTTTAATGTTTTTTGTTTCTAATAATAAACTAAGTTCATTCAAGGAACTGTCTAAACTATTTAATTTTAATTCAAATAGTGTAAGTTCTTCAACGCTAAACTCATCCAAGAGATTGTTTTCTTTTATAAATTGATAATTTTTAATAAATTCTAAATTTGCTTTTGCAATACGTTCTTGATTATATACGTCGAGATTTGTTATTCTATTTGCCGAATTATAAAAATCTCTTTGAATTTTGCCATCTTCGAATAAATAATAAGATTCAATGGTTTCAATAGCTTTTAAAAAATCACAAATATTTTCAATTTTTTTTAAATAAATTATTATTTTTTGTTTTCTAATTATTTTTTTAAATTCTAATCCGTGAGAATTTAAAATTGATAAAGCATTTTCTGCATTTATTAAATCATTAAATTTTAACTCTAGGTGATTTGTTCTTCCATTTATTATCGAACCCGAAGAAAGGAATAAACCAGCAAAGAATTTTCCTTGATATTTAACGCTTATGTTAGCTAAAAAATTTGTATTTATTGAGATCTCATTTTTTTTAGTATACGTATAAGAAATTTTTAGTTCATTACAAATATTTAATATATATGTCCTAATCTTAATATTATTTATTACTAATTTAGCTTCATTGTTTTTTACATTACAACAATAAATTATGCCAGCCATCAAAAAAAACTTTTCTTGTTTTTTTAACTGAAAAGACATAATTTCTTCTTTTACTAGTTGACTAAAAGATTTATTCTCCATAAATGATTTCTTTCCCTCAATATATTAAATTATATTATATAGGAGTCTTTATTTAATTTTTATGCCTGCATTAGCCATTAAATTTAAAGCAAAATCGTGATATTCTTGAGAATTATGATTTGGAATATTAAATGTTGCAATTAAATTTTTAAAGACAATAATTTCTTTATTTTGCTTTAAATGATTGAAATAAGTTTTTAGAGTTATTGCAAATTGTAAAATGCAAATATCTGAACAACAACCTATAATTTCAAAGGAATCATACTTGCTTAAAATTTCCTTTTTTAATGCAAAAAAGGAGTTAGTAGAATTCTTTTCAATTATATTTTTAGCATATTTTTTTAATTCATCTACTACTTGGCTTTCTTTACTATTTTTTAAGCAATGTATTGGATATGATTGCATTTCAATATCATCGCTACTATGAGCATCATTTAAAAAATATATATCATTGTCTTTATATTGAGTTTCTAAAAAATTTTTTATAGGAACAATTATTGACTCAATATTGTCACTATATAGTGCCCCTTCTTTGCAAAAACCATTAACCATATCAACAACAAAAATTGCTTTCATATTGTTTACTCCTTCTTTTTCATTTTTCAATGTTTTTGTATTGTCATATAAATTGTTAATTATTGTATAATTATAACGGATACATCTTAGAGGGACATTTATTTTTAATTATATGTTGGTGTATCTAAAGTTCGAATTTTATTTATTTTAATATATAAGAAAGGATTTCTAATGGCCGAAGAACAATTAGTTGTTACTTCAAGTCCTAAGAAAAAGATTGGCTTCTTTTCAGCTATTCTTATCGTTATGGGTTCGGCTATCGGTGTTGGGGTTTTCCTACGTTCAAAGAACGTTTTACAAAACTCAGCTAACAACGTCGTATGAGCATTGATCGTTTGATTAATTGCTGGATTTGCCGTAATTACCATGGGTATTGCATTAGTTGAAGTTGCATCAGGACGTAATGACAACTTAGGTGTTATAGGTTGAACCAAGGCATTTAGTACATTAAGTCTATACAAAGGTACTAAGTTCTTTATGACCTATCTATATCTACCATTTACTTACTTCTTTATGCCTTACTACGTTATCGTTCAATTCCAAGACGGTATCGCAGGATTCGTAGGATACAGCAAAGTAGGATTTGGTGGTTCTACAAGTGCTCCATGAATCTACTTTGCAATAGGTCTAGCACTTACATTATGAATGATTTTTTCAGCCGGTATTAGTGGCCGTGCTACAAACATTCAAAACTGAATAGTTACAGTTGCAAAATTAATTCCTATTTTCGTAGTTATTATTTTGGGTATTATTTTTGCTGTATACAAAGGTACAGGAAAAGAAGGATTCGTTGCTGGAGTTGATGATGGTGTTACACCACTATGACACAAAATAACATCAGCTGACCTATTCAACAAGAGTTCATCATCATTCTTAGGATTAACACCATTCCTAACTGTATTTAGCTCACTAGGTGGTATCTTCTTTGCATTCGACGGTTTCTATGTAACCGCTGGTATTCAATCAGAAATGAAACACCCAGAAAAAACCCCTGCTGCTATTGTTATTGGTTTAGCATCAATGACTGTTCTATACATGTTAATTGCTGTTACAATGACATTAGGTGGAAACAACGGTTCATTCTACGGATTTGGAGATGAATTAAACGCTGTTAAAGCAGGTTGAGTATTTGGTGTAATTAACATTTTAATCTCAATCGGTATTATTGGTATTTTAAACGGTTTTACCGCTTGAGCTACAAGATGAGTAGAAGACTTAGTTAGAGAAGGTGAAATGTTCATGCCTGTAAAAGCTTACAGATACATTAAACATTCACAAGTTCCATGAGTTGGAGCAATCTTCGTATTATTAATTTCTCTACCATTTATGGTTATCTTCACTGCTATTGGTGCTTACGCATACTTTGGTGGAGGATATTCAACAGACTACGGTTACCAAATTGATAACCTATTAACATTTGCTGACTTGATGGCAAACTGAATGGCTGTGTTCGCATTCGCATTCATTGCAACATCAATTATCGGAATGTTACAAAACAGAAAGAAACACTTTATTGCTGTTGTTGAAGGAAAACACAGTAAATGAACAGGATACGTATCAGTTATCTTCATCTTATCAGTTATGTTCTTCTTAGCAATTGACCCATTCATATCAATAGGTCTATCAGCAGCTCAATACGTTGCAGATAAAACAGAAGACAACAGAAACGGCTTAGTAGGAAACATTGCTACATCAGTTCTATTTATAATATATGCAGCAGTTTCATTCTTAGGCGCTCCAGTTGAAAGACAAATTGCTAAATCTAACAAGATTAAATTTGATAGAGTTCTTTCAGGAAATATTTGTGCACACGAAGTTCCAGAATGATGCCCATGCAAATTAGAAGAATTAAAAGAAAAAGCTGAACTAAATGACATGGTTCTAAAAACATTTGCTGAAGCAAGATAATAATTTGTTATTTCACCAGTGGGTTACTCCCACTGGTTTTTATTTATATTTTTAATATTCAATTAAAAACTTAATTTTAATGTTAAAATGTAAAGATAATTATTTTAATAAAGGTAACCAATGAAAAATAGTAAAGAACAGATTAACAATATTCCTTATGTTTTAGATGAAACATTGATTAACACAAATTTAAAAACCGGCCTAAGTTCGCAAGAGGCCGCCTTAAGACTTAACAAGTTTGGAAAAAATGAGTTGCATAAAGCAAAAAAAATAAATCCATTTTTAATATATTTAGAACAATTTAAAGATATATTGGTCATTATATTATTATTTGCAGCAATAATATCTTATGCTCTTGCGATTGTTTCAGGAACTCAAAATAATTGAAATTGATCTATCAATGGTAATCGCTTATTAATTGAATTTATTGAACCTACAATTATATTATTTGTAGTTCTCACTAATTCTCTAGTAGGCGCTATTCAAGAAATTAAGAGCATACAAGCAGTTGAGGCATTGAGCAAATTAAGTCCCTTACAAGCTAAAGTCGTCCGTGATGGCAATTTAATAACATTAGCATCAAGTGATATAACCGTTGGCGATATTGTTTTAATTGAATCAGGTGATGTTGTACCTTCTGATGGTTATTTGTTGTTTAGTTCAAATTTAACTGTTATTGAGAGTGCATTAACAGGAGAAAGTGAACCGGCGACAAAAGATTACCAAGCTAAAAGAAATTTAACATTAGCAATGGCGGATAGACAATTTATGGTTTATTCTTCGACTGTGGTTGCTACAGGAACAGCTTATTTTGTTGTTACATCAGTAGGTGAAAATAACGAAATCGGAAAAATTTCTAAATTAGTTAATCAACAAAAAGAATCAATGACTCCTCTACAAATTAAAATCAATAAATTAGGAAAAATATTTGGATATTCAGGTCTTGCATTATTTTTAGCAAGTTTAGTGACTCAAATAGTTTTCCATGCTGTATCAAGAAATTCTTTTGCTGATACAATGTTTTGAGGCAATGCAATAGTTGGAGCCATTTCACTAGCAGTTGCTGCCATTCCTGAGGGATTAATTGCTTTTACTTCAATTATTTTAGCAATTGGTGTTCAAAGAATGGCAAGAAAGAATGCGATTGTTAAGAACTTAATGGCTGTTGAGTCTCTTGGCAATTGTGCAGTAATATGTTCAGATAAAACAGGAACATTAACACAAAACAAGATGACAATTGTAGATGCTTTTGATGGAAGTCAACTACTATCAAATAATAGTGAAATTAAAAACTTTTTAAATATTATAGAATTAGGTTCGTTATGTTGCGATGCAAACTTGACTCGCAATGAAAAAGATGAATATGTTGTAGTTGGAGATCCAACAGAAATAGCATTTTTATATGAACTTGAAAAATTTTCTAATTTTAAATATAAAAACAACCTCGAAATAGAATATAAGCGTTTGCATTTGTTCCCGTTTGATTCAGATAGAAAAATGAAATCAGTAATAAATCAAAACGACAACAATTATTGAGCAACGGTTATGGGCGCTCCTGAAATAATAATTGAAAAATGTAATTTTGATTCACCTTCATTAAAAGAAAAATATTTACAAATAAATAATCAATGGGCAAATAATGCTTATAGAGTCTTAGCAATAGCAAGAAAATCCTTAAAAAAGGAAACTATTGAGAAATTCAATGAATCATCAATTTCTAAATTACAAGAGCATGTAGAAAATGATTTAGAAATTGTTGGATTAGTTGCAATGATTGATCCTCCAAGAGAAAGTGCAAAGGATGCTATTACATTATGTAAACAAGCGGGAATAAAACCTGTTATGATTACTGGCGATAATTTAAATACAGCAAAGGCAATAGCAAAAAATCTTGGTATATATAGTGATCAAGACCGCGCTATAACAGGAACTGAACTTTCAAGCATTTCTGACGAAGATTTAGTTCAAAATATTGAAAAATACAGTGTTTATGCTAGAGTTAAACCAGAAGATAAATTAAGAATTGTTAATGCTTGACAACAAAGAAAACAAGTTGTAGCAATGACCGGTGATGGTGTTAATGATGCTCCAGCATTAAAAGCTTCAGATATAGGCTGCGCTATGGGTATAACAGGAACTGAAGCTTCTAAGCAGGCTGCAAATGTTATATTGGCAGATGATAATTTTGCCACCATAGTTTCAGCAGTAGCAAACGGAAGATCAATTTATCAAAAAATTAAAAACGTAATTCAAAGTTTATTGATTACATCGATTGCAGAGATCATTTTACTATTTATAGGTTTGTTTTTATTTAAAGCGATTTTTTCAAGTGCTGAATGAAAAGTGGCATTAGGAAAAAGTGATTTATATATAATGTCAGCCACTCAATTGTTGTGAATCAATTTATTTACTCATGGTTTTCCAGCAATTGCATTAGGACTACAAGATTCTAAAGAAGACTACATGAATAGAAGGCCTATATCAAAATATGAGTCAATATTTGCAAATCAAATGGGATTCAACACTTTATGAAAAGGAATTTTGATAGGAATACTATCACTAATAACTTATTATTTAGCAGCGTTATATGCTAGAAATAATGGATTTAATGCAGAAGATATATTAAAGGCAGCATCTACTTGTTCATTTTTGGTTTTAGGTATATCTGCTACATTCAATTCTATAAACTTAATGACAAAGAAATCAATCTTTATATCAAATCCATTATATTATTGAAAAGTTTATGCCTCAGTAATTTTTTCATTAGCATTTCTATTGATAGTAGTATTTGTAAAAAATATAAATCTAGTGTTTGGATGTTACACCGAAATTCATTCTAAGCCCGAACTTTTGGCACTAGGATTAGCATTACCATTAGTAATTATTCCAATATATTCAATTCATAAATTTATCATATATTTAATTGAAAAAAACAAAATTAAAGAAAATAAAATTACAGAATTTAAAATGATATTACCTCCAAGGCAAGTCAAAAGACATCAAAAAAATGTAGATCTATAGAAATCTACATTTTTTATATTGCAATATAAAATTATACAATTTCATTTTTTGCTTCAATAGCTCCATTAAAGGCTTACGCTCATTACGGTAATGAATATGACAAAGGCAAAAAACATAGTTAAGCAAGAAATAATAATTCCTGCTATTGACAAAGTTCTTCCTGAATCATTCATTGATTTTGACTTGCTAAGCCCAATACAGCTAAAAATAAGACCTAATATTGCAAAGAAAAATGACAATATAAATCCGGCAATAGCAAATCCATTAGTTGGTGAATTTTGGATTTGTTGTTGACGTTGCAAATAAGGTTGAGATGGTTGCGTTGTATTCGTTTGTGTTGTTACATATGCATCTTTTTCCACCTTTTTTCCGCAATTTGGGCAATAATTAGCGTTATTTAATTTAGTTCCACAATTTGGACAATAATTCATACTACCTCCTCTTACTAAAATGCATAAAAATTATTTTTTTAAATTAATTTTATATATAAATATAACACAATAAAGCAATAAAAAAACATTCCAATTCTTGTTGGGATGTTTTTTTATTTAATTAATTATTTTTTTGTTTATATATATTTTATTTTCTATATCAAATGTTTCACCGTCAAGTTGCATAAAGTGCATTTCCTTACATTCCATGCTAAATTTATATCCTTGAATTTCATGTACATATTTTTTATAACGTAAATGTTTTCCACTATATACTGAAAAGAATAATCATGCTAATTTTCATTTAGAGACGTTATTAATAATGATTAAATTTAATTTGTCTGTATTGTTACAATTAGGAGCTAATTTCATTCCACCACCAAAATAACAATTATTCATAATTGAAGCTAATCAAACATTTTTAAATTCTAATTCTTTTCCATCTACATTAATTTTTATTAAAGGAATTGTTTTGAATGTCTTAAATGCATTTATTGTTACTTTAGCAAATGACGATTTATTTTTAAGCATTTTTTCATCATTAACTTTCTTTGCAATAACTGCATCAATACCAAAACCAATGCCATTTAAAAAAGATCTTTCATCTTGGTCGTTTTTAAATTTAATTGTTGGTAAGTTGGTAATGTAAGGAGATATCAAATAATATTTTTTATGTATTATTTGAACATCTTGTTTTATTTTTAAAGACCTTAGAAAATCGTTGCCCGTTCCTGCTTTATATGCATAAATTTTGGGTAAAGGTTTTATATTGTGAATTTTATTGGTGATATTGGTTAATGTTCCATCACCGCCAATTAATAAAACAATATCTTTTTCTATATCCATTTGAGAAACTAGATTTTGACTGTCTTTAATTTTTATAATGTCAAATATTTTTACCTCAGGTTGTAAAAATACTTTAATGGCGTCAGAAACTATGTCATACATCTTTCTTTGACATTTTCCACTCTTTGATAGAGAATTATAAAATATATATAGCATAAATTATTTTTCCAAATCTTGTAATCTATTTAATGCATTGATATAAATTTCAAGTGATGATTTTATTTCATCAAATGTAAAATATTCGTTTGGTCCATGCATTAAATGCATATATTTAGTTGATCCAAAAGCAACACAGGTTTTTATTAATCTTGCAAATGTACCGCCACCAATTGCTAAAGGTTTTTCATTTGAATTCATCGCTTCATTATATGTATCCATAAGAATTCTTACAATTGGAGAATCTTGTTCAATATACTTTGGTTCCTTGAATCCTTGCAATTTTGAAAATACATCATCGCCAAATTCATCTTTTAAATAATCATTTAAGCTTTTAGTTACATCATCAATTTTATGAGAAACTGGGACTCTAAAATCAAAAGTTAAAACATATTCATTTTTCAAGGTTCTAATAATGCCTAAATTTGCAGATAATTCGCCCGAAAAATCACTATAGTTTTTAAAAATATTACTTAAATTAAATGATTTGCTATTTAAATTTTCTTTAATAAATTTAAATAATTTAGATTTAGCAAATTCTTTATTTTCTTTGCTTGCTATATTAATGGCTTTAATTATTGCATTGTCACCTTTTTCAGGTGTTGAGCCGTGTCCACCAATTCCTTTAATAATATTAGGTTTTTCATTCAATACTAAAGATGTTGAATCAGGAATTTGGTTAACAACGTCGCCCCCATTTAATTCAACTCCTGGAACCTTTGGAAAATGAATTTCTGTTACATAGATCATTTTTTCAGCATATATTAAAGGTCATTCACCATCAGGAGTATAAGAAATATAAGGATCTTTGAAGTCAGCCAAATAAGACTTCATTGACTTCATTGTGGTTTCTTCTGAAATTCCAAATATTATTCTTATTTTTCATTCGTTAGATAATAAACCATGATCATGAATGTATTTCATTGCATATAGATTAATAATAGCAGGTCCCTTATCATCTAAACTACCACGACCAATAATTGATTCATTTGTTATAACTGGGACAAATGCACTTGTACGTCATTGGCTTTCATCTCCTGCAGGAACAACGTCAAGGTGGGCCAGTATTCCAATAATCTTATCTCCATTGCCAAATTCAGCAAACCCATAACGATTTTTAGGATCTTTATAAATATTAAATCCAAAACTTTTGGCAAGATTTAATGCATAATTTAAAGCATTGTCAGTTTCTTTTCCAAAAGGAAATTCACTACTAAAATCTTCTTCACTAATCGAAGGAATTGCACAAATGTTTGCAATATTTTTAATCATTTCATCAAAGTCTTCTTTACTTTGCTTATAAGTAATAAATTTTTTCATTCTTACCTCTTATTAATTTTTTTAATATATTATACATTTTAATCCAAATAAAAAAAATAAATAAGGCGTAGCCTTATTATTTGTTTAATCCAACAGCAATACCTTTGTTTTTAAAAACATAGTCATTATTATTGTTATATACAACATCAATTAATTCGGGATAATCAATAAAAGGATTTCTATTTCCTTGGTGTTTATATACTCCATTGTTTCTATCAATGTCAAATTTTGAAACCTTATCTAAATTATTTCAAGCTAAAAATGTTTTTAAAAATATAGGAGTTATAGTATTTTTTCTATTAATTTCTTTAAATACACGAGTTGATTGATTATTTGATGTTATATTTAAATCGTTATAAGTTAAACAAAAGTATAAATATGCCCTAGCAACATCACCTTTAAATTCATTAATAGGTTCGGTAACAGGTCCACCATCTTCAACTGAAGAACCAGTTTTTGTACCATTTTTGGATATTTTATTGCCTTGCAATTCCTTAACTGTTCCATAAGGGTAATTTGCATGCCGTGCATTAACTAATTTATCGGTTGGTCAAACATGGTGAGCATCTGTTCTCATTGGAGCATTTTTTCTAAATCATGATTGTGGAACTAAGTGTTCTCTATTCATTCCTTGGCCTTCACGCTTGCCTGTATCTCTATATCTTCCATGTCAATTAGTAAAAACATCTTCTTTATCTGGGATTTCTTCATAGATATCTAAAACAGATCCATCTTTTTCATAATATTTATCAACAAAAGCTTCTTTATATGTATCAAATAAGTCATTATATTTTGTTGAGTGATTTCTATGAGCTTTTTGCAATGCAAATAAGTTATCTCTTAGGTTTTGTCCACTTAAGCCATCTAAGGATTTATAAAAGTCATTTGAATTATCGTAAATTATCTTATTATCTAAGGGTTTAGCTTCAGGCATCTTAGGTTTTTCTGGTTTAGCTTCAGGGCTCTTTTCGGGGGTTGTTTTGGGTTTTTCACCTTCTAGTTTAGCTTCAGGGCTCTTTTCTGGTGTTGTTTTTGGTTTTTCACCTTCTGGTTTAGCTTCAGGTTTTACACATCCAGCAGCAACTAATGGTGTTGAAATTAGCAAAGGTAAAATAGAACCCATAGTTAGGAATATTTTTTTGGATTTTTTCATTATCTTATATATTTCGCTTTCTTTTTTGTATTTAATAAAAATCTATCATATGAATTAAATAAGTCAATTCAAATTAATAATAATTTTCGAAAAAGCAAATAAAAAAACACCTATAAGTGTTTTTTAATGTTTAACAAAGAGTTTGCAAAGAAAAACTTATTCAGCTTGTTGTTTAGCAAGTCTACGGCGAGCTCTGGCTTTTTCTAAAGCTAATTTATGTTCTCTTTTAGCTGCTTTTACCTTGTTAGCTAATCTAACGTCTTTTCTCATAATTTCTCCTTTGCTTTGTTCAAGCAATAATTATATCTATTTTTTCTTAATTTGTTTAATTTTTTGCATTATTGTTTCAAGATCTATTTTTTCAATTTCTTGTATTTTCTGACTAACGCTTTCTTTTGTTGCTATATTTTTTTTATCTTTTTGGTTGTCATTAACATTATTTGAAACATTTGGTAACTCAATATCTTCTGTAGATATTTGAAATAGTTCTTCTTGTTCATCATTATTTAAATGTTGAATATCTTGATTATAGGATTTTTTAATCAAAGCATGTTCAGCGGTCAATTCAATATTTTTATCAATATATCCTTGATATTCTTGTCTTATTTTTTCCTGCTCTATTTCGTCAGCTTCAATTAATTCATTAATTTTTATTGGCGTAATAAAATACCCAGGGTTAGTAAATGAATAATCTAATTTAAGAAGTTTACTTTCCTTAGTCTTATCTTTTAATATAAATTTTCAATCATATTCTGCCAAATTACCATTATTATCAGTGTATAACATTTTTAAATTGCTCAATGTAACAGCAAGTGATTGAAGACCTAAAATATTATTTTTTAGTGGCGTAAACAACATTTTTGGAGTATTGCGTGTGGTTGATTCTAATTTGCTAGTATCCCAACATTTTATATAGCCACCTTGCGTTAAATAAATTATATTGTTGTATTTTGAAACTGTTATAAAACCACTTACTTGAAGTTGAGGATGTTTATATAAGTTTAAAAGAAGTCTAATGCCTCTTGCTTTCAAAGAAATTCCAACCTTTAGTTCGTTTTCCGAAACTAAATGATACATACCATTATTTAGTAAAATAAATAGCATTTTTTCATCATTAGAAATTTGGGCATCTATTAATTCATCATTATCATCCTTAAACGACATGCAAGTTTTTGTTTTAAGAGAAGATTTATTTTCAAAATCTCTTAAATTAACTTTTTTGGCATATCCTAATTTTGACATTAAAATAAAGTTTGCATAACTATTGAATGACGTAATTTCTATAACTCTAATAATTTTCTCTGAACTTTCTAGGGCTACTATTGATGAAATATGTTGGCCAAGATCTTTTCAATTGCAATCTTTAAATAAATGGCAATCAATAATAAAATAATTTCCTAAATTTGTAAAGAATAATATCTTTGATAATGAGTTTACTTTATCAAAATAGAAAACATTATCTTCTTCTTTTAACTTGAACGTGCTTAATTCATTGCTAGTATAAACTTTTAAACTTATTTTTTTATAATATCCATCTTTGGTTATATATAAATAAAAATCTTCATTTTTTGTCAATAATTTATGATCAATTTCTTTATCGATTTTTTGATCTGAAATTTCCGTTAATCTTGGCTTTGAATATTGATTTTTTATTTCAAGCAATTGCTTTATTAAATATTGATTAAATTCTCATTTATCATTTAATAATTTATTGCAATTTTCAATTTGAATTTCAAGGTTTTTCTTTTCTTCTTGAAATTCAATTTGATCCATTCTTGAAAGCTTGTACAATCTTAATTCAGCAATCGCTGTTGCTTGCAATTCACTAAATTTAAATTTATTCATCAATGCTAGGATAACACCTTTTTTTGAGTTATCGCTTTCTTTAATCAAATGTATAACTTCATCAGAAATTTCAGCTACTTTAATGAACCCTTCAACTATTTCTAGCCTCAATTTAAACTTCTTTAAATCATAATTAATACCATTTATATTAACTTCTTTTAAATGACTTAAATAAGCAATTAAAGCACTATAGAGATTTAGAATTACTGGACGGTTATTGTCGATTGCAACCATGTTATAACTATACGAGATACTTAGTTCGGTTTTATTCATTAAGTATGCAATTATTGCGTCAGCATTTGCACCGTCTTCTAATTGAATAAATATTGAAATTCCGTTTCTATCTGTTTGGTCCAAAACTTCTTTAATACCAGAAATTTTTTTATCTATTGCAATTGCATCAATGTCGGCAACTAATTTTGATTTAACAACCCCAAAAGGAATTTCAATTATTTCAATACCAATAATTTTCGATTCATCTTTTTTATCATAAACATAATGATATTTCGAAGAAATTGTAATTCTACCTTGCCCACTTGAAAGAGCTTTTTTTATTTCATTTATACCATTAATAATTGCTCCTGTTGGGAAATCCGGTCCTTTAACTATTTCACTTAATTCTTCAATTGATATTGTAGGATTTTTGATTAATGCAATTGTTGCATCAATAACTTCGCCTAGATTATGTGGTGGGATTTCTGTAGCAAAACCAGCAGCAATTCCTTTAGCACCGTTAACTAATAAATTAGGAAATAAGGCCGGCAAAACAATTGGTTCGTATTCAGAGTCATCAAAGTTTGGAGCCATTTTTACAACTTTTCTGTCTAAATCTCTCAACATTAGTTCACTAATTTTTTCAAGTCTTGATTCAGTGTAACGCATTGCAGCGGCAGGGTCATCATCAATTGAACCTTTATTACCATGCATTTCAATTAATGGGAAATTGCTTTTTCAATCTTGAGCCATTCTGACTAACGCTTCGTATATTGAACTATCTCCATGAGGGTGATAACGTCCGATAACATCCCCAACGATTCTAGCTGATTTTTTAAAAGGTTCGCTATTTTTTAAATGTAAATTTCACATTGAATATAAAATCCGACGTTGAACAGGTTTTAGTCCATCACGAGCATCAGGAATTGCTCTTTGTTGAATAATGTATTTTGAATAACGCCCGAATCTATCAGACATTATATCGGCCATATTTTTTTCAATAATGTTTTCAGTAACTTCTTGTATTTCTTCTTTTCTATCTTTTTTCATACGACCTACTTATTATTTATTTTAAAATCATCTTCTGATGAAAAATCAACATTATTGTTTATTCATTGTTTTCTTGCTATTACGTCGTCACTCATCAATGTTGAAACATTTCTTTCAACTAAAATTTCATCTTCTACATCAACTTTAATTATTGAACGAGTTTCGGGGTTCATTGTTGTTTCTCAAAGCTGGTCAGCATTCATCTCACCAAGCCCTTTGTAGCGTTGAATTTCATAGTTGGTATATTTTGAAGTAATTTCTTTTAATTGGTCTTCATCCCAAGCATAAAGAGGCTGTGTATTTTTTGATTTTATTGAAATCTTATAAAGAGGTGGCAAAGCAATATAAACCATACCATTTTCAACAAGTTTTTTCATATAACGTCAAAAGAATGTTAATAGTAATGTTTGAATGTGAGCACCATCAGTATCAGCATCTGTCATAATAATAATTTTGTGATATTGAGCATTTTTTAAATTGAAGTCTTCGCCTATACCTGCTCCGATTGTATTAATTATTGTTGCAATTTCTTCATTTGCTAAAACATCGCTTAATTTTGCTTTATCAGTGTTAATAACTTTACCTCTAAGTGGCAATATTGCCTGGGTAACTCTATTTCTTCCTAATTTAGCAGAACCGCCAGCTGAATCGCCTTCAACTAAAAACAATTCTTTAATGGCAGGATTTTTACTTTGAGCAGGAGTTAATTTTCCTGAAAGAATCTTTCTATTCTCAAATTTATTTTTAATCTTACGTGCTTCATTTCTTGCGTTTCTAGCTGCAACTTTTGCTTCGTATGCTTTTTTAATTTTAGAAATAATTTTTTCTGCTTCAATTTTGTTTTCATTTAAAAAGAAATAAAACTTTTGACTTACAAAATCTTCAACAACTTCTCTAGCCTCTGGCGTACCTAATTTATCTTTCGTTTGACCTACAAATTCTAATATTTCTTCTGGGACTTTTAAAGAAATAACAGCAATTATTCCTTCTCTAACATCCGATCCTTCAAAAGTTATTTTCTTAGCTAAACCATTTTTAGAAGCATAATCATTTATTGTCTTAGTTAATGCGGTTTTAAGTGCTGTTTCATGTGTTCCACCATCACGAGTCTTTACATTATTTACAAAACTGTAAATTGATTCACTATATCCATCAGTATATACTAATGCCAAGTCAATTTCAATGCCGTAATTGCACATACCAGATGCATAAAAAATTTCACTAATAAATTTTTTGCCTTCACCAACAAAATTTATATATTCTTTTATGCCATCTTTGGTTTTAAATGTTACATGTTCATCAGTATTTTCATTATAAAATTCAATAGTAAGATTTGGCATTAAAAAACTGGCTTCTCTTAATTTTTCTTTAATTATTTCAGGTGAATATTTTGATTTTTTAAATATTGAAAAGTCGGCTCAAAATTGAACCTTTGTTCCTTGTTTTGTAGTTGGAGATATCTCATGTGTTTTTTCGATAATTGTATCTTGCTGAAAGGCTGTAAAGTATGCTTTTTTATCTCTTCAAACCGTAACTTCAACTTTAGAACTTAAGGCATTGACAACTGAGGATCCTACTCCGTGTAATCCCCCACTTGTTTTATAAGCGCCATCGCCAAATTTACCCCCAGCATGAAGCTCAGTAAAGACAAGTTCAACTCCACTTTTTCCAGACTCATGTTTATTGACAGGGATTCCCCTACCGTTGTCTTCAACAATAACAGAAGAATCCTTCTTTAAAGTTACAATTATTTTATTTGCAAACCCACTCAACGCTTCATCAATAGCATTATCAAAAATTTCCCAAATTAAGTGATGAAGCCCACTTGAATCAGTAGACCCAATATACATTCCCGGTCTTTTTCTGACGGCATCTAACCCTTTTAAAACTTTTAAATCGCTAGCTTCGTAATTATTTAAACTCATATCTAAATTATAAAGAAAAAAAATCTCTTGAACTATTTAAAGTAATAAGAGACTTATTTTAGAAAAATTTTGCTTGCGATTTTTATAATTTGTGGATTTGAAAGATTAAAGTTTCTAATGTCTTTAAAGTCAAAAAAAGCAATGTACAAATCAAAACTATTGTCATCATTAATTGCAAAAATTAATTTAGACCCAATTGGATATTTTTCTAATTGTTTAAGGTCTGGCATTTTGACGTCTAACATTGCCAAGGTCGTGGCAATGTTGGTATCGTGGCCTACAATCAATGTTAAATCTTTGCCGTTGTTGAATTCTTTTTTAATTAATTTATATATATTTTTTTTATTTGAATTTATAACATTTTTGTCGCAAAAAGCAAAGTCAATTACATAATCCTTAGCCTTACCTATTAATCTTAAATCTCTTATAAAATTATTAGATTTAAATATTGAATCATATGGTTCGTTGAAGTAGTATTTAACTACTAATAAATCACTTAAAGAACAAGCCATAAAGAACTTGCCTCTTCAAAAAACTCCATCTTTATTTTCATTCAATGTAATATTGGTTTCTTCATTTGTAAATAAACAATTTTTTAGGTCAAAAAGGTCAATAATTTTTTGATATATTTTTTTACTTTTTGCTTCATATCTTCATAACAAATCATAATCAACGAGATTAATATCGTCAGTGTTAATGTCATATCATTCATTCTCGTTTTTGAATGAATAGTCTTGACATTGAATGTCAACCTTTTTAGCGGGAAAAAGACCAATTGAAAGCAGTTGTGCAGTTTGAAAAGTTCTTTGCGTTGAATTTGCCATCACCTTAACATCAATATCATTTGTTATATTGAAAAATTCTCTAATCTTTTGGGCCATTTTTAGCTCAAAAAGTGCTCCTTTATCTGTTAAAAATGCACTTCCTAAATTATCGATGTTTCAATTTACAATATCCTTATTGAAATATTTAATTGAACGTTCTTTTGTAAAAAACGGATATCTTAAACCATGCCTACTCAAAATTAATAGTTTCACAATGTCACCTCATTTTTTCAATAATTACGAGTAATTATTTTTTACATAATTGCATAATCTTATATAATTATATTGTTATATAAATAATTTTTAATTATATAAATTTTAAGGAGAAAAGATGAAAAAAACTAGAATTGCAATCAACGGTTTTGGTAGAATTGGTCGTCTAATTTTTAGAGAAATTTTTAATGACCCTGAATTTGAAATCGTTGCTATTAATGATTTAACAGATGCTGCTACATTAGCTCACCTATTGAAATACGATACAGCTCATGGAAAATTGAATGAAAATATTTCACACACAGAAAATTCTATTATTGTAGATGGCAAAGAATATCGTGTTTATAGTGAAAAAGATCCATTAAACCTACCTTGAAAAGAACTAAACGTAGATATAGTTATTGAAGGAACTGGAAGATATGTAACAAAAGAAGGTGCTGAATTACATATTCAAGCAGGCGCTAAAAAGGTATTTATTACTGCTCCAGCTAAAAGCGAAGGCGTTAAAACAGTTGTTTATTCAGTAAACGAAGATATAATTACACCAGAAGATAAAATTTTATCAGGCGCTTCATGTACTACTAACTGTTTAGCTCCTATTGCCAACGTATTGGAAAAAAACTTTGGTATTGAAAAAGGATTTATGACAACAGTTCACTCATATACAGCAGACCAAAGATTACAAGATGCTCCACACAAAGATTTAAGAAGAGCAAGAGCAGCTGCTTCAAACATGGTTCCAACAACAACAGGTGCTGCAATAGCTATTGGAAAAGTTATCCCTTCATTGGATAAGAAACTAAATGGACTATCTTTAAGAGTTCCAACAATAACTGGATCATTCGTTGATTTAACTGTTGAATTAAAGAAAGATGCCACTGTTCAAGAAATTAATGAAGCAATTAAAAAATCTGCTTCTGAATCATTAGAATATTCTACTGAACCAATTGTATCAAGTGATATTATTGGTGCAAAAGCAGGCTCAATATTTGACTCACTTTTAACAAGCGTGTTAGAAGTAGACGGCAAAAAACTATACAAAGTTTATGCATGATACGACAATGAATCTTCATTTGTTGCTCAATATGTAAGAACATTAAGACATATTGGAAAATTAACAAAATAGTTAAAAAAATATTGGATAAGAAATGAAACTTTCAATAAAGGAAATAATTAAAAAAATTAAATTACTTGAATTGGATCTCGAAGATTTAAAAAAAGAAGAATCCAAAGAAAACTTTATTGTTTATTTAGAAAACAAAAGGCCAACTAACATTGAATATGATTATCAAGATTATTCAAACAAAATTAAAAATTTATATAATGAGATTTTTAAATTAAGAACATTGGTTCAAGTAACTAATATAAACACAATAACATCATACAAAAAATATTCTATATCAGAATTAATTATTTTATTAGCTCAAAAAAGTAATCAATTAGAAAGATTGCAAGAAATGCGCGATTGCAAGAAAATTTCTAGGGTTACTACAAATGACGGTCAAAATGAATATACAGAATATCTTTTTGACCCTAAAATGGTTGCAATTGAACTTAGAAACTTAAACAAAGAAATTTCTGAAATTCAAATTGCAATTGATAGTGCAAACATTAATACATTAGTTGAAGTTAAGTAATTAACTTTCCTTAATAAATTTGCTTTATTTTCTATAATTTTTTGTTTATTGTTATAGATTTCTAAATTATTAACCTTCCGTTTTAAGTTTGTGTTTATATGATATCGAATTTACATACTTAAATTAGCTAATTTATTTAAAAACTTCTACATTTAGTAAGGGTTAGAAAATGTAAACCACCATTTGAGGTGGTTTTTATTTATATAAATTTATAAATATTAATAATTAATAATATAATTAGATAATTATGAATACAACAAAAGTATATATTGATTTTGAAGCAATTACCAACCCTTTTGCAAGATTAGTAAATTTGCCAAGTGGAACTCCTTATGCCTATACATTAGGTCTTTTAAATATTCACGGTCAATATCAAATTAAAACATTTATTATTGACTTTAAAGAACATCATAATTTAAATTCAATTTGAACTGCTATAAAAAATGCAATAGTTTCTGACATATTAGAGATCAATAATACTGTAAATTTAAAGGAAGTTGTATTTGTAGGCCACAATCCTGTTTTAGAAAATAAATGCTTAAAAAAATTATTTCCTGAAAATAATGTTGAACCTTTAATTTCCAATACAATTGTGTCATTGTCAAAATTAACAGGAAAAATTTTTAACATTCCATATTTTAATAGAATAAAAAAAATCATTGCTGATTCTGGTGATAGTGAACTTAATATGAGAATTAAAGATAGAAATGGCGTAATTGCCGCATTTGCTGGGTATTGATTATATACAAAATCAATTAAAAATTTACGAGCAAATGATAGAAGAAAAAGGTTTTTAATTAAGATGAATAAAAATCTTTTGTTAAAAGAATTAAAACGTTATTCTCACGACGATGTCAACAAAATGCTTTATATTGTTTCTCATCCTGATGAAACAGAAACTCTACTAAAGGAACTTTTATACAAACGTGAATTAATAAAACAAATAAAAAATTTGGAACTTGATGACAATTTAACAATTAAAGAAATTAAAGAAAAAATTTGAACATTATAATTTCTAAAAATAAAGGATATTATGAAAAATAAGCTTATTGAAAAAAAAGGTAAGTTTTCAATTAAAAAATTTTTCCCTCAAAGCAAGTTTGATTGAAAAATTTATATTATAAAAACATTGCCAATAATAATTGGCGAAATTATATTTAATTTAAATGGGCTACTTGACAATTTCATGGTTAGCCATATTGATGGCGGAATTGATGCCTTGGCTTATGCTAATACATATACGGGAATAATTTATACAATTTTCTTTTCTATTCAAGCAATTGGAGCAATGTTCGTGGGTCAATATTATGGCCGTAAAGACTTTGACAAAGTAAAGCAGATAATGAATTTAAGAATTTGAATGAATCTAATTATTGCCTTAAGTTTTGCAATTCCTGCTTGAACTTTAGCAAAGCAAATGGTTCACTTGATTGGTGGGCAAGGAATAAAACCTCAATCTGAATTAGCATCTACATATTATTTAATGTTTATTGCCATAAACTGAGTTTTAACTTCTTTTAATTTTAATACAAATATGTTGCTAAATGAAACCGGACATTCAAAATATGCTTTTGTTTCGGCATGTTTAACAATGCTAACAAATGCTTCAATTAATTCTATTTGTTTATACGGATTTCATAAACCTGCATATTATGCTGCTTTTGGTTCAATTATTGCTGCGGCAGTTTGCTTTACAAGTGATTCTTTATTTACATATTACAAAGAAAGAAAAATTTTTATTAATGTATTTAAATTGTTTCATATTACAAAACCAATTGCAAAGCAATTATTAAAAAGAATTCCCGCCATGTTAATTGCCATTGCCGGAATGTTAACTTTACCTACAAGAATGATTATTTGAAGCCATGCATATCCCGATAATTTATCAGGTGGTTCAGGAATTGGTGAAAAATGAATGGGAATTAATGCTGTTACAATACTTGGTTTAGTCGAAAGCCTTTCTTCCGTTGCTTCGGCAATAACTTCTGTTTGTGCAAGCAATGTTTCGTTTTTTGTTGCTACAAGATTAGGAGAGTCTGATTTTGAAGGCGCAGATAAGCATGCTCATTCATTGCGTGGATTTCATGCTCTGGCCGGATGTTGTATGTCTATATTAATGATTGCGGTTGTTTTTGGAATTGCATATTCTCCTGCTACAACAAAAGGTGTTGGTATCGGTGTCTCTCAAAGATTTTATGATGCACAAAGAAAAGATTACATTATTGAAATATTAAATAAATCTGATATTTTAAAAAATAGTGATTTATTAAACTCGCTTGGCAATGATTTTACATTCACAAAAGGCCAAAATTTGGCTTATGATCAATGAGTGGCAAGCGCAGTGCTGGCAACTTCAAAAGAATTTAAAGAAACATTTTTATTATGTAGCGCAACCTTTATTTTCTTTAACCCTATATGATGTTGATTTTATACAAGCGCCGCTTTGCCTGCTGCGGGTGGTAGAAATATAATTGGTTCTATTACAATATTAGCAACACAATGAGCATCATTTATATGATTGATTATTATTACTTATGGAATTGTTATTCCATTGCATAAAACTTATACTGGATATAGTGATTTATCAATCCCTCTAGAACTTGCTTATTTTATGTTATTTGCATTTGACTTTATTAGATGATTGATGTTTGAAATAGTGGCATTAAAGACTGATTGAAAGAGAAACATTACAAATGAAATTGAAAAACAAGACCCAAACTTTCCAAGTAATATAGCAGAAGCAACAGCAACACACGATATTGTTCATAAACAATAAAAAGGAGATTTAAGATGTTATATGATTTAGTTTCACCATACAATTCAGGACATTTAAAGGTTAGTGATATTCATAGTATTTATTATGAAGAATCAGGAAATCCAAATGGCAAGCCAATTTTGTTTGTCCATGGTGGCCCAGGAGGGGGAACCAAACCTTCATGTCGTCAATTTTTTGACCCTGAATTTTATAGAATTATTTTATTTGATCAAAGAGGATGCGGACTTAGCATACCTAGTGCCGAAATCAAACAAAATACAACTCAAGATTTAATAGAAGATATTGAAAAATTACGGAAATTTTTAAACATTGACAAGTGAATATTATTCGGGGGGTCATGAGGTTCAACTTTAAGCTTGCTATATGCAATTAATTATCCTCAAAACGTTTCAGGAATAATTTTGCGAGGAATATTTTTAGGTAGGGATGAAGACAATTATTGATTGTATCAAAAAGGTGCTTCTTATTATTTTCCCGCTGAATATGAAGAATATTCAAACTTTATTCCTGAAAATGAAAGAGGCGATTTAATTAATGCTTATTATAAATATTTAAATTCAAGTGACCAAAATGTTGCAGAGCAAGCAGCATATCATTGAAGCAAATGGGAACTTGGATTGGTAACAATGAAAAAATTGCCTAATTTAGAAGAAATTTTATCCGATAAAAAAAGTAATTTAGAATTAGCAAGATTAGAAAATTATTATTTTGTTAATAAGATATTTTTAAATGACGATAATTTTATTTTAAATAACGCAAGCAAAATCAAAGAAATTCCAACGATATTAATTCATGGTCAATTTGATATGGATTGTCGTCCAGTTGGCGCATATTTATTGCATAAAGCCTTACCAAAATCAAAACTTTGAATAATTCCAGAATCAGGCCATTCAGGTCGTGATGGGCATATCAACGAAGCATTAATGCAGGCAACTGAATACTTTAAAACAATTAAATAAGCGTTTGCTTATTTTATTTTACTAAAAGATTATGAAACAATAAATGCAATATTTCTTATAATTTAAACAGACATTTAAGGAGAGAAATGAATTCAATAAATAACGTTGCTAAAAAATTAAATTTAAGCGAAAATCAAGTAAAAAATACATTATCTTTGTTATCAGAAGGAGCAACAGTACCATTTATTTCAAGATATAGGAAAAATATAACTGGTGGTTTAGACGAAGATCAAATTACAAAAATAAATGATTTATATGTATATGATGTTGAATTAAATAAAAGAAAAGAAGCCATATTGAATATATTGGCCGAAAATAAATTATTAAATGATGAATTAAAACAAAAAATCGAAGCTGCTGATACAAAACAAGCAGTTGAAAATATTTATGAACCATTTAAAATAGGTAAAAAAACAAAAGCATCAGACGCTATTGCTCTTGGTTTAAGTCCGTTGGCTGATGAAATTTTAAATAATAATGATGACAACTATAATCTTTATTATGCTGCTAAGAAATATATTAGTGATAAATTAAAAACTGAAGATGAAGTCTTACAACAAACTAAATTTATCATTGCTCAAAATATTTCTCAAGACCCTTCTACAAGAGAATATGTAAAAAATCAATTATGAGATTATGGAGTTATTGAAACTAAATTAAAGAAGAATGCAATTGATGAAAATGAAACTTTCAAACAATATTATAATTTCTCCGAAAGAGTTAATAAAATTCCAAATCATAGAATTTTAGCAATATCAAGAGGGGAAGATAAAAAAATTCTTTCATATGATATTACTTATAATGAAAAGAAAATTATTTATGATCTAAATCAAAAGTATTTCAAATCAAAACGAACTGCTAAATGTATTCAAGAATGTATTTTAGATGCATTAGACAGACTAATTATGCCTTCAATAATTAGAGAAATTAAAACCGAATTATTTGAAAGAGCTGAGGCAGAAGCTATTAAAGTATTTGCTTCAAATGTTGAACAAATGTTATTGAGTCCAGCAACTAAAAATAAAAGAATTTTAGCAATAGACCCTGCTTATGTTAATGGATGTAAAATTGCAGCCATTGATCAAAATGGTAACTTTTTAAAAGAAGGAATAATTTATCCTCATAATCACAATCAAGAACAATGATTTGAGGCAATTAATACAATTAATAAATTTATTGATGAATACAAAATAGATTTAATTGTTATTGGTAATGGAACAGCAAGTAGAGAAACTGAATCGTTTATTGATAAATTATTGCAAAAAAGACTTGAAAAAAATCCTCATGAAAAAATCAAATTTGTAATTGTAAGTGAAGTTGGTGCTTCTGTTTATTCAGCTTCAAAAATTGCCCAAGAAGAATTTCCGCAATTACATGTTGAATATAGATCAGCAATTCATATTGGCAGAAAGTTCCAAGATCCCTTAAATGAATTAATTAAAATAGATCCTAAATCAATTGGAATAGGACAATATCAACATGATGTTAACCAAAAGGAATTATCAAAACAATTAACCGAAAAATTAAATAAAGTTGTTAACCTAGTTGGTGTTGATCTAAATACAGCAACAAAAGTGATTTTATCTTATATATCAGGATTAAGTAATACCATAGCAAGCAATATTGTAAACTATAGAGAAGAAAACGGTTCATTTAAATCACGTGAAGAACTTAAAAAAGTGAAAGGACTTGGGCCAAAAGCATACGAACAATCAGTTGGATTTTTAAGAATACATGATTCTAATAATTTTTATGACAAGACTAATATTCACCCCGAAAGTTATAAATTAGCAGATACTATTGTCAATAAGCTAAATATAGATTTGAATAATATTGACAAAGAAAAATTATTATCCGTTGATAAAGAACAACTTGCAAAAGAATTAAATTCTGATAAATATAGCATTCAATTAATTATAGAATCATTGATTGCACCCGAAAAAGATATTAGAGATGACAAACCAGGATTTTTAGTATCAGATAAAGTCTTAACTATTGATGATATAACTCAAGGTCAAATAATTAAGGGTCAAATTCAAAATGTTACCGATTTTGGTGCCTTTGCGTTTATTGGATTAAAACAAAACGTTTTGATACATATAACCAATATGAAGAAGAAAGAAAATCAATTTATTAAACACCCTTTAGATGTATTAAATGTTGGTGATAATGTAAACATACAAATAATTTCTATTGACAAAGAACACAATAAAATTCAAGGAAAAATTATTTGAGATTAATTAATAATCAATATTGCAACATTGCTATTGAAAAAATAGCAATGTTTTTTATTGTAATTTTGTAAAATTTATATATAAAAGTTATTTATTGCACTGGGGGGAAATTATGGCTATTAATAATCAAGAACGTGACGAATTACATAAAAAAATTTGAGATATAGCAAATAGATTACGCGGTTCAATTGATGGTTGGGATTTTAAACAATATGTTTTAGGAATTATGTTTTATAGATATATTTCTGAAAATATTGCAACATACGCCAATAATCGCCAACGTCAAGCAGGAATTGAAGACTTTGATTATGCAACTTTATCAGATGAAGAAGCATTGACAGGCAGAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTACCTTCTGAATTGTTTATTAATGTTGTTAGAAATGCTACAACAAATAATTGTTTAAATGAAACGTTGGACAATATTTTTAAAAATATTGAATCATCAGCAAAGGGACAACAAAGTGAAAATGATTTTTCAGGTCTTTTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTGCAAGAAATAGCAGCCATGAAATTAGGCAATTACCAAGATAATTCAATAGATGCCTTTGGCGATGCTTATGAATATTTGATGTCAATGTATGCATCAAATGCTGGAAAATCAGGGGGTGAATATTTTACTCCCCAAGAAGTTTCAGAATTACTTACTAAAATAGCAGTTTTCAATAAGAAAAAAGTCAATAGAGTATATGACCCTGCATGTGGATCGGGTTCTTTACTTTTACAAACAATCAAGGTTCTTGGTAAAGAAAATATCAAGGATGGATTTTATGGCCAAGAAGTCAATTTAACAACATACAACTTATGCAGAATAAATATGTTTTTGCATGATATAGGATTTGATAAATTCAACATTTACAACGGCGACACATTGCTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTCATTGTTTCAAATCCTCCTTATTCAATTAAATGAGAAGGTGAAGATAACCCTTTATTAATAAATGACCAAAGATTTTCACCAGCTGGAATATTAGCTCCAAAAAGTAAAGCCGATTTTGCTTTTATACTTCATAGCCTTTCTTGACTTGCAACTGATGGAGTAGCTGCAATAGTTTGCTTTCCGGGCATTATGTATCGCGGAGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAAGCAACTTGTTTTTTGGAACAAGTATTTCAACATGCATAATGGTATTGAAAAAATCAAAAATTGACAACAATATCTTATTTATTGACGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAACAAGTTAACTAGTCAAAATATTAACAATATTATTGATTACTACAGCCAAAGAAAAGATATTTCTTATATTTCAAAATTAGCTAGTGTTGAAGAAATTAAGTCCAATTCATATAATTTATCAGTCAATAGTTATGTTGAAAAACAAGACACTTCAGAAAAAATAGATATTAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAATATTGAGAAGCGAAATTGACAAAATAGTTGCCGAATTAGAGGAATAGTTATGAGTAAAATAGAAAAGTTAATTAAGGAACTATGTCCTAATGGAGTTGAATATAAAAAAATGCAGGATATCTGCGACATATTTGGAGGTTTTACTCCCCCAAAAAATCTTTCCGAATATTGAAAAAACGAAAATATAAATTGATTTAAACTTGAAGATATTAGACAAAAAGGTCGAGTATTAAAAAGCTCAATTACTAAAGTGGATAATAATGTCTTAAGAATAAAAAAAATTTTTAAAGCAAATTCAATTATATTATCTACTACCGCAACAATTGGTGAACATGCTATTGTATTAGAACCATTTTTTTGCAATCAACAATTTTCAATTTTTACTGTTAAAGATAAATTTGTGGCTAAAATAAACCCAATGTTTATGTTTTATTATTTTTTTATCTTTTCAAAAATCTTAAAGCAAAGATATGAAAACGTAAATTTACCTTTAATTAAAAAAGAGGAACTTGGGAATTTGTTATTCCCCATTCCTAATATTTCAATTCAAAATCAAATTGTTAATATTTTAGACAAGTTCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCTATAGAGATAAACAATATAATTACTATAGAAATAAATTACTAGACTTTGATAATAATAAAAATCTAATAGCCAAAATTATGAATAAAACAACTTCAGATTCTTTAAATAATTTAGTTAATTATTGAAAAATATTAGATTTGTTTGATATTATAATACCTCCTAAAAAAATACCTATTAATGAAACAATGAATAAAGGAAAATATATAGTTATCAATCAAGGCGATGATAAAAAAATGTATACAAATGATGAAAATGCTGTTTTGCCAGCAAATGAATATATTTTGTTTGGTGATCATACAAGAAAAATTAAATTTATAAATCATACCTTTGCACAAGGTGGTGATGGTTTAAAAATTTTATTAAATAAAGATAAAACAAAAAATAATGTATCATCAAAATTTTTATTTTATTCATTAGATAATATTAATATACCTTCTTTAGGATATAAAAGACATTGGTCCTCAATAACGAATATCAAAATCCCTATCCCTCACATTTCCATCCAAAATAAAATTGTTGAAATTCTTGACAAACTTGAAACATACACCAAAAACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTTTGAAACAAAATTGTCAATATAGTTGAATATGATTTAATTAAAAACTTGTGTGAAATAAAGCGAGGAAAAGTTTACTCAAAGGAATTCATAAAATTAAATAAAGGTGAATACCCTGTTTATTCATCTCAAAGTTTAAACGATGGAATTTTAGGAAAAATTGACAAATATGATTTTGATGGTGAATATGTAACATGAACTACAGATGGAGCATATGCCGGGACAATATTTTATAGAATGGGTAGATTCTCAATAACTAATGTGTGTGGAATATTGAGTGTTCCAAATAAATCAAAATTAAATGTGAAATATTTAAGCACTTGCTTATCTATGCAGACAAAAAAATTTGTAAACAAGGCAAGTGGAAATCCTAAACTTATGAGTAATATTATGGAAAATATTGAAATCCCAATCCCTCATATTTCCATCCAAAATAAAATTGTTGAAATTCTTGACAAACTTGAAACATACACCAAAAACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTATGAGACAAAATTGTCAATATAGTTGAATATAAAGAATTAGGAACCATAATCACAAAGGCTCCCAAATCTCCATTTGGAGTTGCAAAACTCTTAAAAATGAACAAAGGGGATTATTTAGCAATAACTTCAGGAAATAAATCATTTTATGTCTCTGATTTTTTAGTTGATGGAGAATATATATTTGTTAATGATGGTGGGCAAGCAGACGTTAAATATAATCAAGGAAAAACAATGTATTCCGATCATGTGTTTGCGTTTAAAACAAATGAATGCAACACAAAATATCTTTACTTTTATTTATTGAATATTTCTAATTTTATTAACGAAAAATTATTTGTTGGTAGTGGATTAAAAAATTTAAATAAAAAAGAATTCTTGAATCTTAATATTGCAATTCCTCCTATTGAAATTCAAAATAAAATAGTTGAAATCCTCGATAAATTGCAAGCCTATACCAAAGACATTCAAACTGGTCTTCCTCTTGAAATTGACCAACGTAGAAAGCAATACGAACATTACAGGGATAAATTATTGAACTTTAAAGAAAAGAATACTAAAATTTAATTAGTAGCAATATTAAATAAGGGTGTAAACTATGGAAAAATATAACATTATTGTTGAAAACGAGAATGATACAGTTGTTTCTGAATATAAACCTTTAGCGAATAAAGCAAAAGATTATCAAACAGAAAGTGAACTTGAAAGTCAATTCATATCAATGCTTAAAAGTCAAGGATATGAGTATTTGAATATTCATAATGAAGAAGATTTAATTAATAATTTAAGACAACAAATTGAATTGCTAAATAAAATAAATTTTTCAGATAATGAATGAAAAAAATTTTTTAATGAAAATATTGCAAATAAAAATTTTTCAATAAAAGATAAAAGTAGAATAATACAAGAGGATTATCGCCAACTTTTAACTAGAGATGATGGAAGCCATAAGAATATTATTTTAATTGATAAAGACAATATTCATAATAATAAACTTCAAGTTATAAATCAATATGAAGAAGAAACAGGCAACTATAAAAATAGATATGATGTTACTATATTGGTTAATGGCATTCCAATGGTTCATATTGAATTAAAAAGAAGAGGGGTTGCTCTAAAAGAGGCTTTTAATCAAATCATTAGATATCAAAGAGATTCGTTTTGGGCTAGTAATGGTTTATATGAATATGTGCAAGTGTTTGTTATTTCAAACGGGGCAAATACCAAATATTATTCAAATACAACAAGATTACAACATGTTAATAAAAAATCAGATATGATAGATTCAAAAACTTCTAAGAGTTTTGAATTTACACATTTTTGATCTGATTCAAAAAATAACCCTATTCATGATTTAATTGATTTCACAAGTACCTTTTTTTCAAAACATACGATTTTAAATATATTGACCAAATATTGTATATTTACATCTGAAGAAATGTTGCTAGTAATGAGACCTTATCAAATTGTTGCTGTTGAAAAAATAATCAATAAAATAAATATTTCACACAATTATAAAAAATATGGAAGTATTGCAGCGGGTGGCTTTATTTGACATACTACAGGAAGTGGTAAGACTTTGACCTCTTTTAAAACTGCAAAATTAGCCTCACAATTGCCTTATATAAATAAAGTAATTTTTGTTGTTGATAGAAGAGATTTAGACTATCAAACAATGAAGGAATATGACAAATTAGAAAAAGGCGCTGCAAATTCAAATCGTTCTACATCAATATTGCAAAGACAACTAGAATCTAATGACCCTAATAAAAAAATAATAATTACAACTATTCAAAAATTATCAATTTTTATAAAAAATAATAGAAATCATCCCGTTTTTGATAAAGAAGTTGTTTTAATATTTGATGAATGTCACCGCTCTCAATTTGGTTCAATGCATACAGCTGTTATTAAAAATTTTAAAAAATATTATTTATTTGGTTTTACCGGAACACCAATTTTTGCAGCTAATATCAATAAATCAAGTCCGGAAGTGATTAAAACAACCGAACAAGCTTTCGGCCAAAAATTGCACACTTATACAATAATAAATGCAATAAATGATAGAAACGTTTTGCCATTTAAGATCGAATATATTAAAACAATTGATGTTGATAAATCGCTAAACGATAAAGAGGATCAAAAAGTAAGAGATATTGATCGGGAAAGTGTTTTTTTAGATCAAAGAAGAATTAAACTAGTTGTTGAATACATATTGAAACATTTCGATCAAAAAACTAGGAGAAATTCCAATATAATTTATAGCCATTCAATATCTGATAATTTAGATAAAACTATTAAAAGAAATGTGAGCGGTTTTAATTCTATTATGGCGGTTTCTTCAATAGAAATGGCTAAAAAGTATTATTTAGAATTTAAAAATCAGCTTGCTCAAGATCCTAAAAACAATCTAAAAATTGCTTTAATATATTCGTATGGGGTTAATACCGAGGAATCATATGGCGGTTTAATCGATGAAGAGGATAATGAAAATACATTGCAATTAAGTAAGCCAGATAGAGATTTTTTGGATGATGCTATTAAAGATTATAACAATATGTTCAACACAAACTTTAATAGTGAAGGTGAACAATTTCAAATGTATTATAAAGATGTTTCTTTAAAACTAAAAAATAAAGATTTAGACATGCTAATTGTAGTAAACATGTTTTTAACTGGTTTTGACGCACCAACATTAAATACATTATGGGTTGATAAGAAACTAAGAGAACATGGTTTAATTCAAGCTTTTTCAAGAACAAATAGAATTCTAAATGATAAAAAGGTATATGGAAATATTGTATGTTTTAGAAATTTAGAAAAAGAAACTGACCAAGCAATTTCAATATTTGGCGATGGTGAAGCCAGTGGAATTGTTAAAATCAAAAATTTCGTTGACTATTATTATGGCTATAATGATAGTCAGCAAGTGCATCACAATGGTTATTGCGATTATATTCAAAAATTAAATAATGAATTTCCTTTAGATAATTGAAATGTAATAAGTGAACAAAAGAATAAAGAATTTATTATATTGTTTGGTAAGGTATTAAAATTAAAAAATATTCTTGCTTCTTTTGACGAATTCATTGGTAAAGAAATCTTAAACGTTAGAGATTTTCAAAATTATTCAAGCAAATATTTGGATATAAAAGATGAATTTAAAAAGATATATAAAGAAGAAATTGCTTCAATTAACGATGATGTAATTTTTGAAACCGAATTATTAAAGACTTACGAAGTTAATATTGATTATATATTGCAAAAGGTAGAAGAGTTTAGAGGAAAGACAATTACTGAAATTAACAATTTAAGAAATGAAATATCTTTAGCTTCTTCTTCATCGCCTGAATTAAGAAGTAAAAAACAATTAATTGAAAAATTCGTTTCAACAATTAATAATAATGAACAAGATATCCCAACTAAATTTCAAGAATTTATGGAACTAGAAAAAAAGAAAGAGCTTGACAAAATTATTAGTGAAGAAAATTTAGATTTTGATAAAACCCATAAATTTATGGATAATTCGTTTGAGTTAGGAGAAATAAAAACTGCTGGTAGTGAGATTGGAGATTTATTGCCTAAAATGCCATTATTTGGTTCAAAAGGAAAAGAAAGATCTATTAAAAAGAAGGCAGTTTTAGAAAAATTTGAAAATTATTTCCAAAAATTTTATATTTAAATTCTAATGTTTTTGGTTTATAATATAGTTACGTTTTATATTGATATATAAAGGGTAGGCTAAATGCCTCCCTTTTTACTTAAAATAGATAAAAAAGGAATGAAAATGAGTGAAATAAAATTAACAGCAAAAGAAATTTACAAACAAATTTATAACCTTTCACAAATTAAAAAAATAGATCAAGCAGAAGTTGTTGAAGCTTTTAAAAATACAGTTACAAAGTTAATAACTGAAACTTATGACGAAGAAGCAGATCTTGAATTTATTTTTGATCAAGAAAATGACAATTTTGCAATTATTAATCATAACAAAGTTGTCGTTGGCGATCCAATAACAAGCGAAGAAAAAGATCGCTTAACAAGATGCATAGAAATACCATTAAGTGATGCTAAAAAAATAGATCCAAATGCAGAAATTGGAGATTCTCTTTCTCAAGAAATTAACTTTGAATATTTTAGTAAAAAAGACTATAACAAAATTCTTGCTAATTTCAGTCAAGATATTAAAAATTTAGAAAGAAAAATGACTTGTGAAAAATATGCAAGTGAAGTTGGAAATTCAACTAAGGCAAAAATTGTTTCTGTTAACAAAGGAAAAGTAAATCTTGAATTAAGAGACGGAACTTTGGCATTTATGCCTTCTAATTTTGTAAATCAAAGAATAATTTCAAAATTAAATCCAGGCGATTGAATTGATGTTGTAATTGAAGAAGTAAAAGAAGAAAACAGCAATGCTCAAATAATTGTTTCGTCGGTTGAATCAAGATTACTTCAAAAATTATTTGAAATTGAAATTCCTGAAATTTCACAAGGCTTAATTTCAATAGTTAATATTGCAAGAATTCCTGGAGAAAGAGCAAAAGTTTCTATTAAAAAATCTAATGATGCTCCTGAAACAATGGAAGAAATCGGCGCAATAATGGGACGTGATTCTGAAAGAATTTCAACAATATCTAGAAAACTTAATGGAGAAAAAATAGATGTAGTTTTATATTCAGATGATATAAAAGAATACATTAAAAATGCATTAAGTCCAGCAAAGGTTATTGATTTAATTGAAGTTCCATCAAAAAATTCTTATCCTTCATTTAATGTTATTGTTCCAACAATTCAACATACGCTTGCTATTGGAAAGAAAGGCCAAAATGTTTCTTTAGCTTCTGAATTAGTAAAAGCAAAATTAGATATTTTAAGCCAAGAACAAGCAGACGAAAGAGGCATTGAATACAATTTTGAAAATGGTAATATTACAAAAGAAGAAATTGAATTACTTGAAAGTGGTAAAAAACTTCATCACTCATTTAAACACCCAAGAACTATTGGCAATAAAAATGCTACAAACAAATTTAATACGTTTGAAAATAGTTTTAATATTGATGAATTTGATGAAGACCTTGCCGAAATGAGAAGAAAAGCTCAACAAAATGATAATATCTTTGAAAGACAAATGTTTTCAAATAGTCTAGATGAAGATTTAGAAAGAACTTTATCAGAACTAAATGACGACAAAGAAAATAATGTTGATGAAAATGACCTTGATCCTTATTCAAAATCTTTTGATGAATTAAATGAACAAAAAAATGATGAAGAAGAGGATTATGAAAAAATTACAGCAACAAAAATGAAAGATTTTAAACGTGATGAAGATTTAAGCAGTGGATTAGAAAACATTGATTTAAGTGATTTGGATGATGAAAAATGGTAAAGTCTATACAAAGAAAGTCAATTGTTGATAATAAAATATATGATATAGATTTATTGCTTAGATTTTCTAAAGATAAAAACAATTTAATTAGCTTTGATCCTTACAAAAAGAACCCCGGAAGAGGCGCATATTGTTTAAACGATAGTGAACAAATTGAAATTCTATTTAAAAGAAGATTATTAAATAAAGCATTTAAGCAGAATATAGATATTCAAAAATACAACGAAATAAGGAAAGAGGTGGACGAATGAGTAAACAAAATATCCAAAAACGAAAATCAAACGTTAGCTCGTTAAAAGAGCAATTAACTGCTGGTGAAACAAAATTACAAGATGGCGTTTTTATATTTAATGGGCCATTGACAATTTCGGAATTTTCTCTTAAAATAAATAAAAATCCTACTGAAATATTGACCTATTATTTTAAAAAAGGAATAATCAAAACAATTAACACAACATTAAGCGAAGATGAAATAGCAGAACTTTGTTTTGAATTTAATTTAGATTTTAAAAAAGAAGAAAATGTTGATGCTCAAAATTTGTTTAATAAGCTTGAAATTGTTGAAGACCCAAATGATTTAGCTTCTAGACCCCCAATAGTAACAATTATGGGACATGTTGACCATGGTAAGACTACATTAATTGATAGGATAAGACATAGTAATGTTACTGAAACAGAATTCGGTGGTATTACTCAACACACCGGAGCATATCAAGTTGAATATCAAGGACGCAAAATTACATTTTTAGATACACCTGGTCATGAGGCCTTTACTGAAATGCGTTCAAGGGGAGCAAAAATTACTGATATAGTAGTCATTGTAGTTGCGGCAGATGATGGAGTTAAACCTCAAACTATTGAAGCAATAGACCATGCAAAAGCAGCCAATGTGCCAATTATTGTTTTTGTTAATAAAATGGATAAGCAGGGAGCCGATATTGAAAGAATAAAAAGCGAACTTGCTGATAACAATGTTTTATGTGAAGAATGAGGTGGAAACTACCAATTTGTTTATGGATCGGCTTTAAAAAATTCTAATATAAATGAACTACTTGAAGCAATATTTTTACAAGCTGATTTACTTGATTTAAAAGCTAATATAAATAGAGATCCTATTGGAACAATTATTGAATCTAGAATTGACAAAGGACGTGGAGTTGTTTCGACTGTTATTGTTGAAAGTGGAACATTGCTACCTCGTGATTTCATTGTAGCTGGAGGGCAATATGGCAAGATTAGAAGTTTAACTTCTATTGATGGTAAGCCTATACAAAAAGCATTGCCTGGTACTCCATGTATAATTACTGGTTTAAGCAAAAACCCTCAATCGGGTGATAAATTTGTAGCTATTAGTGATGAAAAATTTGCTAAAAAATTAGCATCTGAAAAATCATATTTAGATAAATGTAAAGAATTAAATGAAAAAAATGCATTTTCTATTCAAGATGGAATAAAAACTGTAAACATCATTGTTAAGACAGATGTTCAAGGAACAGCAGAAGCAATTAAACAAACATTGCAAAAAATCAGAAATGATGAAATCGCAGTTAATGTTATAAGAGCAACTTCTGGTGAAGTAACAAAAGCAGATATCTTGCTTGCTGAAACTTCTAATGCAAGAATTTATTTATTCAACAATACAATAAATAACGAAATTAAAAAGGCAGCCGAATTAAAGAATATAAAAATCTTTAAACATAATATCATTTATAAAATGGTTGAAGAATTAGAAACTTTAATAACAACCTTAAAAGAACCTGTTTATGAAGAAAAACTAATTGGATCTGCAACCGTCCTACAACTATTCTTTTATTCAAAAATAGGCCAAATTGCAGGATGCATGATGCAAGAAGGCATTGTTAAGGAATTTAGTAAGGTCGAAGTATATAGACGCAATAAATTAATTTTTAAAGGACAAATTATTTCTCTAAAAAGAGGAAAAAATGATGTCAAATCTGTTGAAAAGGGTTTTGATTTTGGAACTCATGTCAAGGATTTTGATAAAATAGAAGTAGATGATGAATTAAAATTTTATGAGGATGTATTAGTCAATGAACACAATTAATCACGAAAGAAAAACACAATTACTATTAGAATTAATTGGAAATAGTTTTTATGAATTAAAAGATTTTGATGTAACAAACATTTCAATAAATGATGTTATATTATCAAATGATGGTTCTCATGCCAAGATCTATGTAACTTTATTTAAAGATCAAAAAAGATATTTAGAAAAATTAACCACAATGACACCATTTATTCGTTCGGTTGTGGCAAAGAATTGAAAATATAAAGTTCTACCAGAATTAATTTTTGAAATTGATACTGTAGAAGAACACGCAGCTAAAATCGATAAAATTCTAAATAAGATTGAAGAAGAAAAAAATAATTAATTAGAAAGCAAAGGAAAAATATGACCAATAATTTTGACGCTATTGTAATTGGCGGTGGTCATGCTGGAATAGAAGCAACTTATGCTTTGGCAAAAAGAAATTTTAAAGTCGCATTGGTAACTTTAAACATTAATAGACTTGCTATGCTTCCTTGCAACCCCTCAATTGGTGGTTCTGCAAAAGGAATAATAACACGTGAAATTGATGCCCTTGGCGGAGTTCAAGGGTTTTTTGCTGATAATGCAATGATTCAAATTAAGATGCTAAATACATCTAAAGGCCCAGCAGTTTGATCATTAAGAGCCCAAATCGATAAAGAAAAGTATTCAGAAATAATTTTAAAAGACATTGAAAAACAAAAAAATATTACATTGATTCAAGATGAAGCAAGCGATTTAATTGTTGAAGATGGAACTTGTGTCGGAATTGAAACACTTGAACATGGTAAACTATATTCAAAAGTTGTTATTATGACAACTGGTGTTTATATGAATTCAAGAATTTTGCGCGGTGAAAATATAAAATATGATGGACCCGATGGAGAAAAGACAAGTTCAACACTTTCAAAAAATTTAAAAAAATATGGCTTTGAAATAATAAGATTAAAAACTGGAACACCTTGCAGAATTTATACAGATTCAATAGATTTTTCAAAAGTTGAAAAAGAAGTTTTAGAAAAAAATGAACTTTGTTTTTCAAGCCGTAGTAATAAAAAACTAGATGAACAAACTTGCTGTTATTTAACACACACAACCTTAAAGACAAAAGAAATAATCTTAAAAAACTTAAAACGTAGTTCATTATATTCTGGAATCATAATTGGAATTGGTCCACGTTATTGTCCAAGTGTTGAAGATAAAATTGTTAGATTTCAAGAAAAAGATACACATCATATTTTCTTTGAACCAGAAACAGCACGTGGTGACATCATGTACATTAATGGGTTGTCTACATCAATGCCTGAAGATGTTCAAGATGAAATGATTGCATCTATTCCAGGTCTTGAACATGCAAGAGTTCAAAAATATGGTTATGCAATTGAATATGATGCAATTAATCCTTTGAATTTATACAAATCTCTTGAAAGCAAAATTTTAAAAAATTTCTTTTCAGCAGGTCAACCAAATGGAACGAGTGGTTATGAAGAAGCAGCAGCTCAAGGATTAATAGCAGGAATTAACGCCGCTAATAAACTTGATAATTTAGAACCACTAATCATAAAAAGAAATGACGGTTATATTGGTGTTTTAATTGATGACATTGTAACTAAAGGCACAAACGAACCTTATAGAATGCTAACAAGTAGGGCAGAATTTAGACTTCTTTTGAGAAATGATAACGTTGATGAACGTTTGTATAAATATGCATATGACAACAAAATGATAAGCAAAGAAGAACATGATAGGATTGAAGAAAAATATAAGATTATTGCCGAAACAGTAGAAAAATTAAAAAATGAATATGTTTCTTCTAAAAGTGATGTTGGTCAAAAGTACAATGTAACAAATGGTATGTCATATTTAAAATTACTTTCAAATCCCGAAGTAGATCCAGTTGATATTTTAGGTTCAAATTTTCCATATATTGATGAAATAACTATTCAAGTTCGTTTGTATGGATATTTAAAGAAACAAGAATCAGCAGCTGATAAAATGCTTAGATTGGAACAATTAAAATTACCAAAAGACATTGATTATTGAAGTGTTGATAATTTAGCAACAGAAGCAAGACAAAAATTAGATAAAATAAGACCTGAAACAATTGGCCAAGCAGCAAGAATAAGTGGAATAAATCCTGCTGATATTCAAATGCTAATATTTTATTTAAACAATAAGCGTTAATAATGAAATTAAATATTGTAGCAGTAGGTTCTCTAAGCAAAGAATATAAAACTATCTATGATCAATATCTTAAAAAAATTAGTTTTTTTTGTTCAATAAATTTGATTGAAATAAAAGAACAAGTTAACAAAAATATTGATTTAAAAATAGCCGAAGAAACAAAATTAATCCTAGAAAAAATTCCTAAAAACTCCAAAGTTTATTATTTATCATTAAGAGGCAAGAAACTTGATAGTGGCGAATTTTCAGATTTACTTATTGAAGATAATATTACTTTTGTAATAGGCGGTAGCAATGGAGTTGATGAAACATATTTTAAAAATAAAATATGTTTTTCAGATTTGACATTTCCTCATCAATTATTTAGAATAATGTTAATTGAACAAATTTATCGAGGATTTAGTATAAAAAACAACATAAAATATCACAAATAAAAGGAAGCGTAGAAAAATGTGGGGTTAATTCCAAATAAGGAAAAAAAGCAAGATCATAAAGGTCTTGCTTTTTTTCTATCATTCATAAAAAAAATAAGTTTAAAAAAACGAAGAGCACAACACAAGCACAGCTCTTCATACATTTACATAAATTATACCACGTGCTCTTCGTTTTTTTTAAAAAACTTAAAAATTTTTGAAAGGAATTTTATGGGTGCTTATAGTGAATTTATGGTAATGCAGCAAGAGGCTAAATTAAACAACGATTATAGCCTTGTGGATGCATATGAAAGAGATTTAAATTGGAGGAGAAATTATAGTAAAAAAATATATGACAAATTAAGCGACATAGCTATAAGCAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATGGTTATTAAATCCTTTAAAAATAAAAAAATCAAAATCATTGATCTAATAAATTTAGGGTTTAAAAAAACTTCTATAAAATCAGCTATAAAAAGACTTATTGAAATAAAAGTTTTAGATGGTGAGTTTTATAAAAAATATAAACTTCTAAAATTAAAAAAAATAGTACTAAAAAACCCTAAGGAAAATTACTGGATTTTAAATGGCTATGATGCCTTAAAAATCTTTCTTCTTAATGGTTTAAGTAATGCTATTTTATATGCAATAAATAGGCATAAATCAAAGCAACTAAATGCTAAAAAATTGCATTGTAAAATAAACTCAAGAAAAGTGATTTTACAAAATGCATATTATGCAAAATTTAACTGATCAAATAGCAAAATATATTTAATGAACAAAGCCATAGCCAATTATTTTGGAGTTGAATATTTGCAAATGTTTGGTATATTGCGTAGTCAAGAAAAAAAAGTTACAACATTAAAATTAGGAAATTTAAACATCAAATGTAATGTTTATAAATTTTCTACACAACGTTATTTGTTAATGCAAATTTAAAAGTTTTAAAGGTTTTTATAAGCAGTAATAAATGGCTCCCAGCATTGGGCTAATGTTTCTGCATTGTAGGTTCAAATCCTACGCGAGCCACCAAATAATATTTACATTTTAAATTACTACTTTTAAAAACGTTTAAATTTAAGTTTTGATAGTTACTGGCACTAAGTGTCTATTTTAGTCATAAATAAATTAGTCAAATTACTAATACAAATTAATAAGAGCGAATATGCGGTCAAAATTTTAAAGATAAATATATAGCAGTAATAAATATAAATAATTACTAATGTTTTAGAAGGGAGAATAAATGGATAAAGTATGAACTGAATTTAATTTTATTACTCCTAACACAATGTATAAAACCAAACTTGGTAATACTCACGATTTTAAAAGTTGAGTTGATTCTGGACTTGTGGTAGATTACATTACAAGACCAGATAAATGCGTTGTATTTAAAGAAGATGAAAATAATTATGATGCTATGAAACTACTTCTTACTAAGGAAGATAAAATAAATTGATATAGACAACATTTAAAAATAAATCAAAATTCTAAACAATCAGGTCTTTATAACTTATTAGATAATGATCCAAAGGATGTAAATTTAGAAAATGTCAAAAACGATTTAAAAAATTTAAAAGGTAATTATTGAGAATTAATCGTTAATCCTGGTGATTTAGGCTTAGATAATAATGTTTTATGTAAGGATAAATGACATGAAGTTTTAAAACCTAATTTTGAAAGATTTTTAAAAGCTAATGCCTTTGATTTAGAAAATGTTAATGTTTTTTATTCTATACATGGAAATACTGATTATCCTCATGTTCATATGTTTTGATATGAAAAGGAGCATAAACACAAAAAAGCCAAACTTAATTTAAATTCAATTAATGTTTTTGGCTATAAAGTGGGTCTATCTATTAAATACGATGAGGATTATCAAAAAATAAATGCACTTACAAAAGACATTTGAAATACAAGAAAACAAATTGTAGGAATAATTGCCAATAATTTTGCTAATAGTAATGAAATTATTTCTAAAAGTAATTTGTGCCAAGAATTCATTAAATCTTCAAAAAGTATTTTAGATGAAGTTTTAAACAAAAAAAATATTTCATATTCTCGTATTTCTGATGAAAATAAGAAAAATATTGAAACAATAAAAAAATTCTTATTAAATTATGACAATGATTATTCAAAGCTCTTTAATTCTTATCAAAAACAAATTAATGATTTAAATAATTTAAACATCGAAGATAAATTTCTAAAACAAAGAATTTCAAAAATTATAAATAATGAAATGGATGATTTTCAAAAACAAGTTGGTAATAAGATAATCAAAACCTTACTAAATGCCTATAAGCAAAATAATAATGCATTTACTAGCCGAAAGAATTTCGCTTCATTTTTCAATGAATTTCTAAAAATATTTAGCCTAAATAGATCGGAATGAGCCCTTAGAAATCTAGCGGAAAGAGAATTTTTGGCTAAAGATTTAAAAGAACTTGAACTAGTAGAAGAGTTTTATCGTAGCAAACTTGTCCATTAGGAGTTTTATGGAAGAACAAGAAAAATTATCAAAATCAAAAATTATAGGAAAGACACTTCTTTCAATAATTGTTAGTACATTTTTGTTTTTTGTTCTAACTTTTATTATTAATGTTTTTATGGGCCAGAATAAAAATCCTGGACATTTTAAACTTTTTTGAAACTCTACTGTTACTTTATTAAAAACCAAACCTGATATTTTTGTTTATATAGGTCTAGGAATTTCTATATTAGTCTTCTGTTTCATTTTTTGAAGGCAAATAAAATCTCTTGGCAAAAAGATATCAAAATACTTATTTGGTGCAAAGGCTAGCCAAAATTGATTATGAAATTATCAATTAGGAGAAGGCTCTAAAACAAAATACAATAAATTATTTAGAAAAATGATTAATGCAGAAACAGAACCAAATTGATTATTTACTTTTGATAAAACTCAAAAATATTGATATGTAAATGATAAAGATGATAACTCTATTGTAAATGGACCGCCAGGATCAGGGAAGACTCATAGAATAATTTTGCCAAATATTGAATTTATGGCACAACTTGATTATTCTAAAAAACCAAATTTAATAATTAGCGATCCAAAGAAAGAAATTATCAAATTTACAGGAGGCTTATTAACTGAAAACGATTACAAAATTTATGTAATTGATTTCTCTGACCCTAAATTATCTCTAAAATGAAACCCTCTTTCATATGCATGAGATTTAGCGGTTGATGGTGAAAAATTCAAGAATTTGACTTTCGAAGAAAGAATTAATAAATCGTTAGAAGTTATAAATAATGTTATTGAAAGTTTAAATTGAGGTAAGGCAGTTAATGGCAATATGTGGATTAGCCAAGCTAAAAAAACATTAATTGTTATTACTAATTTCTTATTGCTATTAGGTATTGATGATAAAAATATTATTCATAAAAATAATTTTAATTTCGAAACAATAGCTTCATTATTAAATACTTCTCACTGAGAAAACGATAAAACTATCTCTGCTTGAATCTTATATGCTAAAAAAAGAGCGCTTGATGACAATAATTATTATTGAAAAAAAATTTATAACAATATAGAAGATTTGTCAAAAACTCCTAAAGAAACATTTGGTGGAATATTAGCCGAAGCACAAAGTGTTTTAAATGTATTCAATAATGATGAAAATTTAAAGATGGTTTTAAGAGACACAGAAAGTTTCAATTTAGTTGATATAGTGTCCGATCCTAAACCTTTTGCTATATTTATACATTATCCTGACCATAAACCTACATCTCACTTTTTAGTAAGTATGTTGGTAGAACAAATTTATCAATCGTTAATTGAACAAGCAAACAAAAATGATAACCTAAAATTAGACAGAAAGGCTTTCTTTCTATTGGATGAATTCGGAAATTTACCTGTTGTTGTAAATTTTGATAATAAACTTACAATTTGTCGTTCAAGAAATATTTTCTTCATGCTGGTAGTACAAGATTACAATCAAATTAAGAAATATAACATATATGGAGAAGGAACAGCCCAAGTAATTAAAAACTCTTGCCAAGTAGTATATTTTTTAGCCTCTAATGATGAGCAAACATTAAACGATATTTCAAAAGCGCTCGGAACAAAAGAGGTTAAATCAAAAAGTTATACAACAAGTGATAACCATTCAAGTTCTACTGAAAGTGAAAAATCCAAACCTGTCATGGAAGTAGCGGAACTTAAATCTAAAAAACCTGATGATTTAATAATTATAAAAAATGGTTATAAACCTTTATTAATTAAAACCTTATTGGCTTATAAATATTTCCCAAATGATAATTATGTTTATACCAGAAACGAAAGTAAGCTACTAACTAATGCGGATTTTGAATTATTTGATATTAGAAGTTTGGAAAAACTTATTAAATTTGATAATTCTAAACAAAAAAAACAAAATAATAAAAACCCAAAAGTTTTAGATGATAACAATTTAACTTATTCAGAAGATGTTGAAGATCCTGCGGATTTATCCCAAGAACAAAAAATTCAAAGAATGAAGATGGTTTTTGGAAAATTAGATCTTTGAAACAATTATATAAATCTAAAAAATGATTATAAACGTACATTAATACCTCATAAAAAAAATCAAATTAAAGAACAAATAGAAGAAACATTAAGTTTAATGTGAGAGCAATATGTTACCAATGATCTCGAAAGCGATTTGCTTAATATTCTTTGATCTGAGGCAAAAGAGCGCAAAATACCAAATTATCACGATTTGAATGTTGATATATTGCTTTACTTGTTGCATCCTACCGAAGACATCTCCAAGTTAATTGAAAAATCTAATTAAGGAATTTTACTATGAATATAAATAAAATAAAAGTTGTTGAATTATTTGGCGGAATAGGCGCTATTAGAAAAGCTCTAATTAGAACCATCAAACCTCCTAATTTTGAAATTATTGATTATGTCGAAAGTGATAAAAAAACAGTAAATGCTTATAATGCAATATATAACGATTTTTACAAACCTTTAGATATAAGAGATTATTCACTTCCTAATAATGTTAATGTTGATTTATTGTTTCACGGATCTCCTTGTCAAGATTTTTCGTGTGCAGGTAAAAATATCGGAGGAAGTAAAGAAAGTAAAAGTCGAAGTAGTTTATTGTGGGAAACAATTAGAATCATTAAAGAAGCTCAAATAAAACCCAAAATTGTTATCTGAGAAAATGTCAAAAATGTATTAAGCAAAAAACATAAACCTATATTTGATGCATATATTAAGGCATTAAATGATCTAGGATATGTTAGCCATTATAAAATAATTAATTCATTAGATTGTGGAATTCCCCAAAGTAGAAATAGAGTTTTTACTGTTTCTATTAATAAAAACTATTTAAAACAATTCAATAATTTAGATACATTAAATTGTCTAAACAATAAATATAAAAATATAAATGATTTTTTACAGCCAAATGTAATAAATCCAAAATACTTTATTAAAGCAAATTGCCCCTCAATGATTAATGCCATAGCAAACCATAAAATAAAAATAATAGAGAATAAAGTCTTTACTATAACGACTAAACAAATGCGTTGAAATAATCAAGGGGTTGTTAAAGTAGAAATTAGCAAAGTTCCTAATAATAGCAATTTAGCAAAAATGATTATAGATAATAAACCATATTATTTAAGGGTTATTACACCATTAGAAGCAATGCTATTAATGGGCTATGATCAGCAAGATTATGAAAGAATTAAGAATTTCTGTGATAGTCAAATTTATAAGTTTGCTGGAAATTCTATTGTTGTTAATGTATTGCAAGAAATAATTAAACACATTTTTAGACACAAACAATAAGGAGAAACATGACAAAAGATAAATATTGTGCTTTTTGTGGAAAAGAACTAGTAGGAATGTATGTTAAGAATTTTTGATCTGATTTTTGTTCAAGAGAATGTAGAGCTAAATCTGAAAAATTGCAAGAAGATTTAAATTATTGGAAATTCCACGAACCGCAAGACGTTTATCCATTAAACGACGCTTGAGAATTAGCAGACAAAGAGTTAAGAAAATACGGAATGGAAGCAGATTTATGGGCGTTTGACATTAATAACATTTATGAAATGCAAAACGATTTTTATGATATTGAATTCGTGGGCATTGACGCCTATGGTAGAGCCTTCATCATAAGCAGAGAAGATGCAGAAAAATATTATGCAAAGTACTATTTAAAACAAAACAAGTTAGTTGAAAAAGGAGATAAATAACTATGGAAATAAAACCATTTAAGATGAATGATATATTGTATATGACTCAATATGACGACGTTTGATATAACGATCATAAGGTACTAAATTATTTTAATAATACTTTATTTAGTTTAAATTCAGAAGAAGAACAAAAACTATATAAAGAACAAATCTGCCAAATTTCTAAACAAATGGAAGAATCAGATGATTTTGTAAATCAATGAATTTATGATAAACTAATAACCTCTGAAACACGTCCAAACTATTTGGAAGGATGTTCTAATATAATTAATACCCTATCTTCAGATAATTGAATAAATGAAAGCGATTGGCTTAAATATCCTGCAAGTCAATTTATGGAAGAAAATGACATTATTCGCAAGGATAATTTAGAAGAAATAATTGATGAAGATTTAAAAGCTAATGGTTATGAAAGTCAATGAATTAATGGAACAACGAAATTTAATGTTCTAAATAATTTGTCAAATGAAGAATATGTTTATGTAAATCCCTATTTAGAAATAGAACCAATTAATATTAAAGAAATTAAAGACGATTACATAGAAGCATTAAAAACTTTTGATTTAGATGACCTTTTAGAATCAAGAGATGAAAAATCATTAATTAATTTAGATCCTAACAAATCTTCTAAATCATCTTTATCTGATTTTGACAAGACCCCAACCGATCTCAAAGATTCCAAAGTAATCAACGAATATAACAATTCATCCCATACCCCCTCTGATACCCAATCAAAATCCCATTCATTAACAAAATAAGAAGGTAATATGAAGAAAAAAATTTTCAATCGTGATCAAATGGAACAAATCCACATTGGTTTAGTCCAAGGTCTTGATGTCACTCAATACACTCAACTAGACCCTCAAGGCCATCCAATATTCAATGATAATCAAATGACCGAAATCCGTAAGGGTCTAAAAAAAGGCCTTGATGTCTCTAAATATAATCAAATAGATCATGATGGATATCCGATATTTAATTGAGGTCAAATGTACGTAATCCGTGAAGGTCTAGAACAAGGCCTTGACATCTCACAATACACCCAACTAGACCTCAAAGGCCATCCAATCTATGATTTTTGACAAATGGAACAAATCCGCATTGGCCTAGAACATGGCCTTGACATCTCAATTTATGCAAAACAAAACAATTTTAATAGACCTATCTATGATTGTTTGCAAATGTCTGAAATCCGTGAAGGTCTAGAACAAGGCCTTGATATATCTCAATACAGCCAACTAGACCATAAAGGTCGCCCAATCTTTAATCGAGGTCAGATGTCTGAAATCCGTGAAGGTCTAGAACAAGGCCTTGATGTCTCACAATACACTCAACTAGACCTTCAAGGCCATCCAATATTCAATGGTTATCAAATGTATGCAATCCGTAAGGGTCTAGAACAAGGGCTTGACATCTCTCAATACACCCATTTGGCCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTCAAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCGGAATCCCAATCAAAATCCCATTCATTAACAAAATAAGAAGGTAAAAAATGATAACCATTATTCTTTTAATTATCATAATCAGACATATAAAAAAGCGCAATAAATTATTAGCAAATTCTAATCAAGCTAATGATCAAAAAAATAGTCCCAAATAAAAATTCTTATTAAATTATGACAATGATTATTCAAAGCTCTTTAATTCCACTCAAAATCCCATTCATTAACAAAATAAGAAGGTAATATGAAAAAAACAATTTTTGATAATGACCAAATGGAACAAATCTGCCTTGGTCTAGAACACGGCCTTGATATCTCTAAATATACTCAATTAAATCATAAAGGCCACCCTATATTTGATAGCTTCCAAATGAATGAAATCCGCGAAGGTCTAGAACACGGCCTTGATGTCACCCAATACACTCAACTAGACCCTCAAGGCCACCCCATCTATGATTGATGACAAATGGAGGAAATCCACAAAGGCCTAAAAAAAGGCCTTGACGTCTCAATTTATGCAAAACAAAACAATTTTAATAGACCTATCTTTGATTGATTACAAATGGAACAAATCCGCATTGGCATAGAACAAGACCTTGATATATCTCAATATAACAAACTAGACCCTCAAGGCCATCCAATTTTCGTTGGTAGCCAAATGTCTGAAATTCGTTATGGCCTAAAACAAGGCCTTGATGTCTCTAAATATACCCAACTAGACTCTCAAGGCCACCCTATATTTGACAGCTTTCAAATGGGTGAAATCCGTAAGGGTCTAAAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGATTTTTGCCAAATGGAACAAATCCGTGAAGGCATAAAACAAGGCCTTGACGTATCTAAACCCACTCAATTAGTCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCCCACTCACAACCTAATCAAAATAAAACCTCAGAATCAAAAAATATAAATAGATGCATATAGATGAATTTATTGATAAATATCGCAGGAATTTAAAACAATCGGGTTTTAATAAAGACAATATAGACTTCTTTTTATATGGTTTAGAACTAGGATTAAATATAAAAATAAAATCCAGTAGAAATTATTACAACTTTAAAAAATCTATACATTATTCTTCAAATAATTCTCATCCCTCTTCCCTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCGGAATCCCTATCAAAATCCCATTCATTAACAAAATAAGAAGGTAATATGAAGAAAAAAATTTTCAATAGTGATCAAATGAATGAAATCCGCATTGGCCTAGAAGAAGGTCTAGACGTATCCAAATACATCCAATTATCCCCTCACGGTCGCCCAATCTATGATAGTTATCAAATGTATGCAATTCGTAAGGGTCTAAAACAAGACCTTGATATCTCTCAATATACCCAATTAAATCATAAAGGTCGCCCAATCTATGATGGTTATGAAATGTCTGTAATCCGTGAAGGTTTAAAAGCTGGCCTTGATGTCTCTCAATATAACCAACTAGACCCTCAAGGCCACCCAATTTTTGATTGAGTTCAAATGTATGCAATCCGTGAAGGTCTAGAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGCTGGTTATGAAATGTCTGTAATCCGTGAAGGTCTAGCCCAAGGCCTTGATATATCTCAATATAACCAACTAGACCCTCAAGGCCACCCAATTTTCGTTGGTAGCCAAATGCACGAAATTCGTAAGGGTCTAGAACAAGGCCTTGATATATCTCAATATACCCAATTAAATCATAAAGGCCATCCAATCTATAATGCTTACCAAATGCACGAAATTCGCATTGGCCTACTATTTGATTTTTTAGGATCATGAGAAGAAAAATCATTAATTAACTTAGATCCTAACGAATCTTCTAAATCATCTTTATCCGATTTTGATAAGACCCCAACGGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCATCCCATACCCCCTCTGATACCCAATCAAAATCAAGAAAGTTATAAGGAACAACGTGTCAAAATACTTTTATAGATTAAGAGGGAGTCAATCTACAGGGAAGAATGCCAAAACATATTCGTTTGGCTCTGCGCAACAATACAATAAAGCATTAATAATGGCTCAAGATGCTATTTCAAAAAATCAATTTAAGGAGGTGCAAATAGAAAGAATACAAGTTATAAAAATTATTAAGCAAAATTCATAGTGTAGGAGAAACAATGATAACAATTATTCTTTTAATTATCATAATTAGACATTTGAAAAAACGCAATAAATTATTAGCAAATTCTAACCAAATTAATAGCGCTAAAGGAGTGATAAATAAATAATTCTCAATTATTTCAAATGTCAATAGATGATGAATGATATACTCAAGAAAAAGATATTAAATACTTTTTAGAAAATTTTAAAATAGACAAGAAAAAAACCATTTGATGCCCCTTTGATACACAGCAAAGTAATTTTGTAATTGTTTTAAAATCATTGGGCTATAAAGTAATATATTCGCATATTGATAATGGGCAAGATTTTTATAAATATGAGCCTATTGAAAATTATGATTTAATAATATCGAATCCTCCGTTTAGAAATAAAGCAAATATTATTAAAAGATTGCAAGAACTAAATAAACCGTTTGCTTTAATTTTTGGAGTTCAATGTTTTAATTCTGGCGGTTTTGTAAGCCAATTGCAAAAACTAAAAAATTTAGAGCTTGTGTTCTTAACAAAAAGAATTAAATTTCTTAAAAATTATAAACAAGACCTTAAAAACATTCCTCAGCCTACTTTTCATTCTCTATGAATTTGTAGTGGAATTACAAATAAACCTCTTTCTATTTTAGAAGGCGCTAAATAAAACAAATTATAAATATTAAATATAAGGAGGATTAAAAATTACTAAACAACAAAAATTACTATTGCCTTTTTGGCTAACTTGAAAAAGTTTTATTTGATATCTTATATTTTTAATTATCGTTTGATTATTTTTAGCATTTTATTTTTCGGGTATGACTATTTCTGTTGAAAAAGGGTCATGGACAAACAAACAATTCAATATTGCACTACTTATAATTTGTTTAATATTAGCCTCAACATTAATTTATCCATTTATATGAGGTAGAAAAAGACGAAAATTTGCAAAATCACTTGTTTAGTTTTATAATTATAATATACTTAAAATAAATAAAAAATAAGCAAATTTCAATACAAAAAATAAACAAATAATAAAGAATAAAAATTGTCATTATAAGACTTAAAAAATATATAAATAATATAAGGAAGTATATGAAGAAATCAAAAAGAATTTTATTAACAATAGGCTCTACAATTGCCTTAACTACTTTACCAATTATGGCAATGAGTTGCGAATGACTTGATAAATTGATTAATAAAAATAATCCTAATCCTACCCCAACACCGGGTCCAGATTCGGGTTTAGTAAATCCAAATCCTTCAAACCCAACTCCTGCTCCTGCTCCAGTTCCAACCCCTGAAGTTAAACCAGAAGAAAAAGAAGGTTATGTTATTTATAAATCGACAAATGAAGATTATTTAAAAAAGAATAACACTTATTATTTTAGCAAATTGAGTTATAACCCCGTGGAATTGTCTGATGAGATTTATAATAGTCAAAAATGAAAACTATACAGACCTAAAATGTGACTTTCTTTAAAAATGCCTAAAGAAAATAAACCAGAACTTGGACCAGAAGAGCAATTAGCCAAATGAGGTATGGATTTAAATTTAAATGCTGACCATTTTACAAATGACTGTGAAAGCGCTTGTGGAATTTGAAACATTATTAGAAAATTGGCTGAAAGTGATGAAAACAAAGAAGGTATCAACCAAGGCGGTCGTTTTAAACCAAAAGATAATGAAAGTTATAATGAAGCCTTAATGAGATTTTTAAAAGAAATGGATGATTATTTAGATCCTGATATTGCATTTAAAGGTTTAACAATGGATGAAACTCATATAAATTATGTAACGGAAAATTTAGCTAAAGATCCTAAATGACTTGAAAAAAAACTTATTTACTCTGCTGATTCAAAAATGCCTTCTACTTTTATTAGAGACGAATTTAACCACCAATCAATTGAAGACATGGATGGATTTGTTAAAGAATTAATATCTGATTATGAAAATGCTATGTATGAAGGATGAACCGTTGAAAATAAAGCAAATGATTGGTTTGTTGCTCCTTTTTCATTTGGATCTAATCCTACAATGAGAAATAAATTTCCACAACAAACTTATGATATGTATACTGATAAAAATGGTCTAGATAAAACAACTCCTACTCCTGCTTATAATAACTACAATCCAGTAGCATTTAATTTCTTAGTAATGGAAAGATTTGACTTATCAGAAAAATTAAATTTTAAAGTTTATAAGAATTTAATTCAATGACTAAAATCATTTAATAATATAAACTCTGAAAATAAATTAAAATGAAGTGAAATCGAAAGTGATAAAAAAAGAAAATTAATGAAGGCTTCAACTGAATTAGTAAAAGCGTTAGTGGAATTTATTGACTGACATCATTTACTACTAGGAGAATTTGAAGATCCTAATGATAGACATTCAACTTTAGCTGATTTGTTTGCAATGAAGAGTTTAACAATAGGTGACAAAATTGACTTTGATTACACATATAGATTTGCTTATAAATTTTTATATGAAAAATTAGTGCCTACAATGGTGGCAAACGGTTGAAACATGCCAATTGAATTTAATATTTTAGAAACGCTTATGCTAGACAGAAATAAAAAAGATTCATATATTTATGACTATATCTTTAAATATATAAATGTCATTTTTAAAGCTAAATATAAAGACATTGATGAAATCAAAAAACCAAATGGATATTTAGATTTAAATAATAAAGAACATTGACAAAAATTATTTTTAGACAATGTTTTAAAAAAATGAAATTCAGCGTTTAATATAAATGTTGATTTAAAAGAATTTTAACTTATATAAGGTGTTTAAAACACCACAACTTATTTTTAATTTTGAATGATAGAAAAAAGCAATTTGATTTTCAAATTGTTAGTTTTCTATTTATTTTTTTAATTTTTAAAATAACTCTTTAATGAGTATGAAAGGAAAAAAATGAAAATCGATGATAAAAGCTTTAGCTTTTTAGGAAATTTTAGTGAAACTATAATGAGGTCTATTTTTATAGCCCTTATTCCAGTTGCAATTATTGTATTAATAGGAATGATATTGTGAGCAAGAAGTCAAAATGCTGCCACAAAAGACCCTAATGAAAGAAAGCAAAATAATTTAAAGGTATGAGGTGCTTTATCTGCACTAATTATTTTAGTAGTAATGTGACCATTAATTGAATTAATTAAGGCTAATGTTAAAAAACCATCTAATGTTGGCTCACAAGCATCATTATTTACACATGCAATATTAAATAATTTTACAATTTCAATACCTAAATTGACTAATTTTAATTTAATGGTGTAATATGAAATTTTTACAGGCGATAATAAATCCTTTAATAAATGTAATCGCAGCGCCTATTAGGCTTATGTGGTTTGTACCTCTTGCTGCTATTGGATTAATAGGATTTACTATATTTAATCTTTTGTTTTTCTTGTTTAAATTGTTTAGCCTCGATTTAAACAACTATTTAATGTTTAATTTATTACCAGGCCAAAGTTATAGCCAATTTTCTGTAGCAAGGCCTTGATATCAATTTTTAATTATTAGTTTTGTATTATGATTAATAAACTTTTTTGTATTTGTTATTAGGTATTCAAAAGAATCTAATGGATTAAAGGCTAAGGAAATATTTGTTCAAGCCTTAAAATCAACGGTAGGAGGCGTCCTCACGCTACTATTATTCCAAGTAGTAATTCTCTTTATAAATATTATCTTTATTGAATTAACAAAAGCCTTATATGGAGATTATAACGATTTCTTTAATTTTGGTTTCGGTCAAATTTCAAATAATATTATTAGAAATATGATGCCCCCAAAAGCCTTTGTACCTTCAAGATATTGAGATCCATTCCCTTACGGATCTCCTATAGCAAATCCTTTGTGAGGTTTGGGTTTTCCTGTCTGATGAAAATTTACAGAAAACATTACAGGTTATGCACTTACTATTGGCTTATCAATAGCTATTGTGGGTGTATTAATATCAATTCCTTTAATATTTGTATTGTTTGACATTATTTCAAGAGTATTTGTTAATTGATTACTATTTATTTCATTCCCACTAATTCCTGCATTTTCTATAAATGATGGAGGAAAGAGAATAAGAGTATGAAAGGAAAAATATTTTGGTAATTTATTATCCTGCTCGATTTATGGCGTGGGAATTATGATGCTGGGTAGTTTTATAGGCTTAGCTAATACATTTATGACTAGCGCTACAAATTATATGATAGGAGCCTTTTCTTCATTCTCAAACTTTACTACTATCAAAGCCGTTTTAGCATTATTATCAACTTGTTTCTTGGTAATTGGCTCTGCATTTGCTTTCAAAAAATTATCAGAAATTGCAATTGCTTTTGTTGGAAGTGAAGTTTCTGGTGGCCAATCTGCAAGACTTGCTATGTCTGGAGCAAGATCAGGGGCAGGAGCATACAAAACAGTAAAGGATGCAGCATTAAAGGGCACGAATGTAGGTGCAGCATATAAAGTTGCAAGTTTTCTAAAATAGCAAAGTATAAGGAGTAATATGACGCAATTACAGCCAAGTAGAATAAAACATAGAAGTAGTAAAATTTGAAAAACTTTCAAAACAAAAGATCTTATTGAATTTGTTGTAATTCTATTATTATCATTTTTTATTAGTTTTGGTTTTAGCCAATTAATAAATGTGAAATTAATTTTCTTTGCATTTTGAATACCTACTTTTTTATTAGGAATTATTTTAATAATGCCAAATCGAAAGAATAATTGCAAGGTATATGTTTTAATATGAAGAATGTTTGTTTTTTGAACTATACCAAAACGGTTTGTAAAAAACGTTCAAAATAATTCATTTAGAAGTAGTTTGGATTTAAATCCTTATTCATGTTTAGTTAATGAATATGTAGTCAAAAATAAAAATTCAAAAATAAGTTTTTATAATCCAAGAAATGATAAAGAAAACTATTTAGCGGTATTTAAACTAAATGGTCTTAATATTTGAAACGAGGATGAACTAACTCGTGAAGCATTTATTGATAGTTTTGCAAAAACTTTAAATATACTTACTTACAAAGTTTCTTTTGTTAAACTTGAAGAAAAAGTAAATTATGAAGAAAATATTAAATATATTGAAGCCAAACAAGCAAAGCAAATAAATAATAATGAAATGTGAAACAATTATTATGACCTTAATATAAAAGATTTCTCTAATTTAAATAAAGAAGCAATAAGTAATAACTTTTATTTAATAGTAAATGGTGAAAATCTTAATGACTTAAATGAGTATCTCGAAGTTATAATTGATAAATTTCAAGATACTGGAATAGGTTTAGAAAAATTGCATAAAGCAAGACTTCTAACATTTCTTAATAAACTTAATATGTTTGGTATTGATAGCCAAAACATACAAGAATATTGCAATGATTTAGATGCTAAATTTGGAGTTGAAAATTCATTAGATAACTTATTTATTTATAATCAAATTATTATTAAAGATAAATATTTACAAATCAATGATAATTACGTAAAAATCAATTGTTTAAACAAATTGCCATTTGAATTGTCTGATAAATGAATCAAAACCTTATTTTCTATTAACGGAACTTGTATATGAAATAATTTCCCTTGAAATAACAATTTTGCCAATAAAAAAATGGACAAATCCATGATGGCATCTATTGATACTACTAACGCAACAGGATCGGTGTCAGCAAACTTGGTTGGTTCTATTGATGAACAAGCGACAATGGCGATGATGTATCAAGTAAATAAAGATGAATACAAACTATTTGATAGTAATTTCTTCTTTGTATCAAAAGCCACTGACCTTGCTGAATTAAATAGACAGCATAGAATTGCTAGAAATATAGCAAGTAAAAAGAATATAAGCTTAAATGACTTAAAATATAGACAGCTTTTGGGTTTATCTGAGGCTTGCAATTATCCTTTTGAAAGATTAGAAAGAGAATATTATCAAATTACATCTATTAATTTAGCTTTGGGATGGCCTTTTGAAACCGAAAACCACAATGATTCAAATAATTTACTTCTAGGTTACTCAGAAGCCACAAGAAGTCCTCTAAGTTTTAGATTATTTGATATGTATCATTCAAATAGAACTAATTTTAATATGTTTATAAATGGTACTAGTGGAAAAGGTAAAACAACTTTTACTATGAAGTTATTAACCAGCATTTTAGCAGCAAATAATAAAGTTGTTGTTATTGACCCTCAAGGAGAATATAGAGAATTAGCTCAAAAACTTAATGGACAAATTATTGATTTGGGAGCAGGTAAAAACACCATAATCAATCCTCTTCAAATCCGTAATTCATTAAAGGATGCAACGGGTAATGATACATCTTCACTAATTAACAACCATTTGTCGTGATTAGAAAGTTTTTTTAAGGTTTTAATTAATATAGAAGATCATAAGTGAATATTGCTTCAAAACGTAATAAAAGAATTTTATGAAGATAGAGGAATATATAAACTTGCTTCTATTGAGCAATTAAAGGCCGTAGAATGGCCAACAATAAGTGAGTTGATAAAATATATGTCCAACTATAAAACGCTTCATTTAAACAATCCTGAAGCAAAAAGAGCAATATTAGTTGAACTTTGTGAATCGTTTAGCTTTTTGTTTGAAAATAATGGAAGATATCAAAACTTATTTAATGGACATACTAATATAAAACTTGATAATGATTTTGTAGTATTTAATACGCAAAACTTAATAAATGGAGCGGATAATATCGGCGCTAAATTAGGAACAATGTGCTTATTAAACTTTGTTAGTGAAATAGTATTTAATAATTGAACACATAATGAAATGTTAAAGAGAAAATATTTGCAAGAACATAACATTAAGTTAATGGATGCAAAACAAGTAGAAGATACTATTAGATTTTGTGCCGTCATCGTAGATGAAGCCCATTTATACATCGACCCTGATAATCCTGCCATTTTAAAATATATGGTTCAAATGACAAAAACGGTTAGAAAATTCAATGCAGGTATGATATTTACTACCCAAAATCCTGGTGATTTTACTATTAATAATGCCACAAGTGATGATGCATCTAGAATTATTCAAAACTGTCAATATTCTGTATTCTTTGGCCTAAAAGACCAAGATATTGACAAAGTGCAACAATTATTTAAAAACTCTAATCAATTATTACAAAGTGAAATTAGATATTTAGTAAATGCAAGATATGGAAAATGTTTATTTTCAATAAGTTCAAATAAAAGAATTAGAGTTGAAATTTATTACAACAAATTAGAACAAGAATTATTCTTCAAGTTCTTAAATAAAGAATGAATTTAATATAGCACATGTGCGAGCATAATACACGCACACGTGCATAAGGAGGTTTAGTTGAAAACTAATAAAAACGAATTAGATCAAGATTTTGATAATTTAGATAATAATGAAATTTTATCAAATGACAATCAAACTCAAAATGAAGATGACTTTATAGAAAATATTAATTTAACAAGTGATAATCAAATAAATAACATTATGAATTCTATCACTAGTTCCCAACCTAAAAAGCAAAAGGAAAAAAAGCAACAAAACAATTTTGAAGCACAATTTTCAGGACGATTTACTGATCCTGAAGATGTAGGAATGTTTTATCGTTGAAAAAAAGAATTAGAAAAGAATGGAAAAACTGTTTATAAAGAATTAAACAATGTTATTAGAAGTTATTTATTAGCAAATGAGAAAAAAGAAATTATGCGGGATTGAAAACATGATTTGTTTCAAGCACTAAGAAAAGTTAGTTGAAGTGCATTAAGTCCTTATCAATTGCAAATAAATCGAAGATTTAGAAGCATGGATATAGAAATGTCTATTATTAATTTAAAACTTAATATGATTATTAATGCTTTATTTAAAGATAATGGAGATTTAGAAAAACAAATTGATAATCCCGGGGCTGATATATTAGCAGAATCTAAGTTCTTTGAACGCACTATAAGAGGTGCTGTTAATAAATTAACTAATGATTGAACTAAAAAAATGGCAAATAAAATTCAAAATTCAGAACGTGCTTGAAATGATTTTCTTTCAAGTTTGAGTGAAAATGAATTTGATGACATTTTACAAGAAATAGGGGCAACTAATTCTACTGCCCAAGAACAAATAGAAGAAAAATAAAAATATATAGCACATGTGCGTGCATAATACACGCACGCGTGCAATGGAGGATAAATGAATAAAGTTATATTAACTGGTAGGCTAGTAAATGATAATTGAAAACCTTTTAAAAGCGAAAAAGGACAAGCATTGCCCTTTGTTATTGCACTATTTGAACAAGCAAATAAAACCAATTTTATCGAGATTACTTGTTTTAATAAATTGGCAGAACATGTTATGAAATATGTTAAAAAAGGAGATTTATTAGAAGTTGTTGGCAAATTACACAATGCTAAATTTAGTTCAAAGAATAACGAAACTATTTATAAAACAGAAATAATTGCTAAAAATATTACTTTTTTGACAAAGTCAAAATCAAATATTCAACCCATTTCAAAAGAAACCAACTCAGAAAATAATGAAAAAGACATTTTTGGAATAGATTTTTTTGAAGATGAAGAAAATTAAAAAAACCTTATTGATAGCCGGAGGAATATTTAGTATTGCTTCGGCTTCTCTATTTTCTTTGAGTTTTTCTAGAAATGAACATTATTCAAGTTATAAATATGAAGAAATTGACAAAGACCAATATTCTTTTTTAAACAACAAAAATTTATTAAAAAGTTTTTTTACAAATTTGAGATATTGAAAAAATAGCAGGCAAGATAGTTTTATTTTTGACCCCAAAAGTCCTACAAGTAATCCCCTTTATTCTGATCAAAGATTTATTGCAAATACCAATGATAGATTAGAATTTTTAAAAGAATTGTCTATTAATGATATTAATAAAATAAATAATCAACCTTTTGGTGATTATAAAATTACTTTACAAAATCAAGAAGAGGCAAAGGAACAATTGCCAAATGTAAAATGGTGAGCTCCTAGTTGATTGTATGGCTACAAAAAATATATCAACGACTTAACTTTTTATCACGGCGATGGTAATGAGTGGTGTGATCAAGATGGGTGCCAGGAAGAAACACATAGCAACGGCGAAATTACTCAAACTATTAAAAAATCTAATTATAGTATTAACTGAGCTAATGCCCAATCTTTTAAAAAATTCACCAAAGATTATGATATAAATATTAAAAAATTCAATAATAGACTTAGTGATCCAAAAATTAGTTCGTCTGATATTGCATATTCTGATGAAGGTTATACTGCTTATAATAAATTTAAATTGTCATACAATGATTATTTAGAAGTTAATTATGATTTTACAAAGTGACATGGCATAGATCTTGAAGATGTTTATTCTTTAATTTGCTTTTTAGCAAATTCTAATAATTTTAAGCATTCTCAAAAATTCATGTCAGCATTTCATCTACAAAATTCAAGTCAAGAAATTATTATAGATTCTGATACTATGGGAAAATTAACGACAGGTATTGATTTAAATATTTTTAAAGGTGGTAAAAGTAATCTTGAAAAACTACAAGAAATTGTTGAACAAATGGATAATGAAACAGCAATTGGCAATTTTATTATTAAATATGGTTATGAAGTCAAACCTATTTCAGATGGAAATCATTTTAATCTAATTTTTAAAATCAAACAATTAAAATATAAGGGAAAAGATATCACTAATTCTTTTGCTTCTAAAATTTTTAATGAAGCAGATACTTATAACTTTGCTAATAATTTTGCCAAAAATCAACAAAGTAAAATTCAAATTAAGAATCTTTATCATATGTTTTATAGTCTTTTTAAAGGCAAGACAATAATGGCTAATGTTCCAGTAAGATTTACTCCAAGCCAAATTTATCAAACTTATGCAGACAAGACAGGATTTGTTAGTAGATTATGATTTAAATATGGTGTAGTTCTTAATCAAAATAACCTATCTAGTATTCAAGAAAATCATAGTTTCAATTTCAAAGACAACCTACAAGAAGACAAACCTATACAATTAGAGCCTGCAAATGGTAATTACGGGGGAAAATGGTTAGTGCATACTCCACTACAAGTAAATTTTGACACCACATTAGAAGAAAATGAAGTTTTATTTATAAATGGTCAAAAAGTTGATGTTTTAAATAGACATTTTGTTTATCATTTAAAAGATTTAAGACGTTCGGCATCCGATGAAGAAAAAATAGCATTAGAGAAAAATGATTCAAATAAGCCTCAAGAAGAGTTAAATGACGATAATTCAAGAAAGAAAAACGAGTATATAGTTGAAATAAAGAAATTCAAACCTGGCACAAATAATAGCGGAGAGCCAATAGCAACTTATAAAATTAAGTTCGTTATAGTTGAACAACCAATCAACCAATCATTTAAATGGTTTGCTTGAGACCCTGAAAACAACCCAAAACAAAAGGAACTAATTACTAAAGAAATAACAAAAGATGGAAGAATTTTAGTGGATAAAGAAGGTAATCCATTACCTAACCCTAAATATGATCCTACAATAGACCCTAAAACCGGTACTAAAAATCAACTCATATGAATAAAAAACGGAACTTTAGCTAAAGAAATAACAAAAAATTTAAAATTTTGATATCCCGATATTAAAATGTTGAATGATTTTGGTATTTTTGCAGAGGGCGCTGTCCTTGGTAAAGGAGCCTTAAGAAATCTAATCTTAGATAAAAATATTGCCAAAAAATCTAATGTTACATACTATAAATGCAAAATTTATGATAAAGGAAGCAACAAATGGCTTAGTCCTAGTGAAATTAAACCTGAACCATTATCTTCATCAAACGGTAATAGTGAAAATGCTTATATGTCTGAAGAAGGCATCTGAATAGTTTATGCTGCTACTTCTACATCAATTTCAAATATTAAATTAGTGCTTATCGATGAATCAAATTCACCAAAAAATCTTTTCTGGCAAGAATTAGAAAATAAAAAAGATAATTATGGCATTGATTTAATGAAAAAATTTGAGCCATTTTGACAATCAACACTTGGAATATATTTTAAAAAATGGTTAATTGAATCTAAACATTATGATAAAGAACAATTAGACAAATTAACATATGATGAATTATTGCCTCTTTATAACGAATGCGTTAATTCATCTTGAAAATTTGCTGATAACGATAATAGCAGCATAGAAAATGCCATTAATATTTTTAACGACTTTAAATGGAGTAAATTACAAAATTTTAATGGTCTAACTGATAAACAAGCCATAAAAGACAAAGTTCTTGAATATATTAGAACAAATATGTCAAGCAACACATTAACCAAAAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACCGAGGAAAATAAATTTCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGACACGTATATAATCCACCTATTGATTTAAGCACGTTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCAGAAGATGTTATTGATGAAAGATATAGAAAAGCAATAGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCCAATTTAAATGAAGTAATTGCCCATTTAATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTCTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGATTGGAATTAGTTTCGAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAGGCTCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTCGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATATAAGAAAATTCTTTTATTACCTACAAACAAAACCACCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATAGACCCTGATTGAAAACCAAATCCAAATCCCGATGATGAGGATAAGGCAGTTAATATTTTTAACGACTTTAAATGGAGTAAATTACAAAATTTTAATGGTCTAACTGATAAACAAGCCATAAAAGACAAAGTTCTTGAATATATTAGAACAAATATGTCAAGCAACACATTAACCAAAAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACTGAGGAAAATAAATTCCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGACACGTATATAATCCACCTATTGATTTAAGCACGTTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCAGAAGATGTTATTGATGAAAGATATAGAAAAGCAATAGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCCAATTTAAATGAAGTAATTGCCCATTTAATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTTTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGATTGGAATTAGTTTCAAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAAGCCCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTTGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATACAAAAAAATTCTTTTATTACCAACAAACAAAACAATCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATTGACCCTGATTGAAAACCAAATCCAAATCCCGATGATGAGGATAAGGTCAGTCCCGAAGATATACCTAAAGACCCAAATAGAATAAAGAAAGTCGTGAAAACTTGATGATTTCCTTTAGTGGTAATTGTAGGTATATTAACATTTTTAGTTGCTTCTATTTTCATAATTAAATCTTTATTAAGGAAATACGGTTGATCATTTGGAAAAAGAGCTAAACGTATTAAACGTCAAAATGAAAACAAGCAATCTTATCAAGAAAAACGCTCAAATAAGCGTCAAGAAAAATTAGCCAAAAAGGAAGCAAAAAATTTAAAACGCCAAACAAAAAAACAAGAAAAAGCAAGCTTAAAGAAAGAAAAAAGGACAAAAAAACAAAAAATAAACCAAAAATAAATAATGAGCAAAATTCTGTAAATTAGCAAATAACATCAAGTGTTCTTGCACTTGGTGTTTTTAATTAATTAAATCCTTTAAAAATATAAAAAAATCTCTTACTAAATTAACTTATAATTTTTTTAGTAAAAGATTATCAATTTGTTTAAGAACAAACATATGCAAAAAAGATTTGAAAAATTTTTAATTTTTTTGTTTATTTGTAGTATAATATAACCAATAGTCTAAGAAGGATTTTGTTATATGAGTAATCGAACAAGCAAGCAGCTCATATTTTATCTTAAGTGCTTGATGATACGCCTTAGACTCTAGTTCGTCTTAAAAAGAGGTTTATTCCTCTTTTTTTGTATAATTTTTATATATGTTAGAATCATGAAAACCATTAAAGATAAAAAATCCTTTACTAGAAAATATTTTTAAGTTGCCGATCGATGGCAAAGTTTTAAATAACGGAAAAATCTAAGCTCACCCCCTTATATTTTTTGAAAATATTATAAAATTAAAAATTTATTTGTAGTATAATTATTATAACTTAAGAAAGGATATTTATAGGTAATCGAATAGTAGGCAGCCTATAAACTTTAATTTTTTGTAAAGCCTAATGATAGGTCTTAAGTCTTAGTTCGTCTTAAAAAGAGGTTTATTCCTCTTTTTTTGTATAATTTTTATATGAGTTTAGAACTATGAAAACCATTAAAAATTACTAATCCTTTATTAGAAGATATTTTTCATCTAAATATCGACACCAAATTTAAAATTCATCCTTTGATAATTTTTGAAAATAAAGAAAATAAAACCTATTATTGTATAAGACTTCAAACATCTACCAAAACTACAAAAAAGCATAATTTACAAATTGATAATAGTTCTTATCAAACTAATGAATATTGAATGAATCATCAAAGTGTAGTAGTCACCAAAGATATTTTTATAATTGATAAAGATGTTTTAGAAGCCAATGTAGATCAAAACATCTATCAAGAAACCTTGACATTAAATAAAAACGATAAATCTTTAATAATTAGCGATTTAGAACAAAGGATTAATTCAATTCCACCTGATTTAAATATTTTAAAAATATCTAATAACTTAAAAAGCAATTTAGTTTTATACACTAATGATAGCCTAGTTAATTCTCAGTCTAATTCGATTTTGAAAGGCAAAAATTACAAAATTAAAAAGGCTACTAAAAATTATTACAAACATAATTTTAACAAATCTCAATTTCTTAAAGATACAAACACGTCAAATATAGAACATACTAAGCAAGCCCTAAAAAACATAAAGTTATGCATAAATAAAGCTAAAAAAATTGATTATACTAATTTTGAATATATTTTAGATGTTCAAACAAACACATATCTATTTCATCTAATTCCCTCTTCTCTATCCGATTTTGATAAAACACCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACCAATCTCAGAAAGAGTCCAAAGCTAGCCAATTCCGCTCAAAACCTCATAATCTAAAACATTAGGCAAGAATATTCTTGCTTTTTTGTTTTAATTTTTATATGGAAATAGATTTTTATATTAATGGTGTAAGCGAAAAAAGTATCTTATCCTCAGGCCAATTAATAGTAGAAAAATTGAACATCGAAGAAGAAATATTTCGTACTAAAACAAGGTTTGAAAATAATCAATATAGTAATTGAATTGTATTTGAAAGAAAGCATAAAACATTAATAATAAATGGAGAAAAATTAACAATTAATATTACTAGATATTTAGACAAAAAAACTAACAAAAAATTCATTTTTTATCATAACGAAATTCTTAAAATAATGGGTAAAAAAAGATATTGATTAAGTGATGTTTTAACGGCAATAAAAACCCAAAATTTAAGGCGTAGAAATCCAAATCAAAACGAAGCATTAAAACCCAAAATTCCTTATGATATTTACTACTATTATTTAAAGAAAAAATACATTACTTTTTTAGTAAAAAATACTAAATTTTTACCTTCAAAAAATGATGTAATTCAGCTAGAAAATGATGATGCTTATATTAATTTAAATAAGGGAAAATTTATGGTTAGAATGGTTGGATTTCACTCTGGATATTTAAACCAAAATAACCATATGTTATTAAATAAAACCATTATTTTTCAGTTTAACAAAAGTCAAAAAATTTATCAAAATATAGATATCTTAGCCACTAAAACCAGCGAAATTTTAAATAACTTTTACCATGCAAAAACTATTAATTTATCAGGAGATGGAGCAAGAATAATAACTAAATTTAGCGAACAAATTAATGCTATTAGGTATTATGATAAGTTCCATTTTCACAAAATATTATTTGATGTTTTTGGGTATTCAAGAAAGAAAAATAAGGATAATAAGAGAATATTTGATAATACATTTGGGAATCAATTTTCTCAATTTTTGTGATTTTTAAATAACAAAAAATATGAAGATTTTGAAAATAAATTACATGAGAATTTGATTTATTTAAGAGAAAATAAATTATCTGCTATAAAAATTGTTGAAATAAAAAGTCTAATAAAATTATGAAAAAACAACAAAAATTACTTAATTAATAGCTATGAAAATGCTAATTATTATGGAGGAAATGCCGAAACTTTTATTGGACATTACCTCAAGAAATATACAAAAACAGCATTTTCGAGACATAATATTGACTATTTAAAACTAAAAATCTTGTTAAATATTCCCCAAAATTTAAATGTGGTATTTTTATAAAAACCACTTTTTTTTATATATTTTAAAATCCCTTGTTTGCTAATGCTACGCTATTATTATATAATTATTTATAATTATATAAAAAATTGAATTGATAGCAATTAGCATGCTATTTTTTAAAAAAACTTAATAATGCAAACTTTTGTGCATACTATTATAATATATTATTACAAGTAATAATATATTAAAAAAGGAAAATCCACACTACGGTAAGTAGTTTCCGTGGTTCTTCCTTTGCCCTATTCAAGGGTGCGCCCGTACGTACGCGGGCGCGTAGAGCATTTTTTAAGTTTGCAATAAAATTTGCATCAAAAAAATGCGAATTTTTTAAAAAATATCTTTAATTCAGTATAATTAGGGTGATAGAAAAATCTCTTTATGATTTTGCGACCCCTGATTTTCAAACACTTCCAAATAAAATAGGCTTTTATTTTAAAAAACAAGTCTATTTTTTTATTTCTTATTATTTTATTTAAAAGGAATAAAACATTTATAATTTAAATGTAACCATGTTAAATTTAAAAAATAAATTCTTGAAAGGAGTTTACATGGCAAAAAATACATGAGAAATTGCCAAACAGGCAATTGAAGAACACGATAATATTTTTATATTTCATCATATTAGGCCCGACGGAGATTGCTTAGGCTCTCAATTTGGTTTGAGGGAATTAATAAAGGAAAATTATCCTGAAAAAAACGTTTTTGTTTTAGGGGATAATGGAGGAATCTTTCCATTTTTAGAATGAGATTTTGATAATTTTGAAAATATTGATAAAAAATATCTTAAGAATTCTTTAGGGGTAGTAGTTGATGCTAACTGCTCAAATAGAATTCAATTTGCTAATTATATTTTAGATGGCAATTTTACCCATATGTTAAGAATAGATCACCATCCAGTTGATCCAGATATTAATTATGACTATACTTGGGAAGATGCAACATATGCAGCAGCTGCTGAACAAGTTGGATACATTGCAATGATGGCAAAATGAAAAGTTACAACAAAAGCTGCTAAATACGTTTATTTAGGCATAAACACAGATTCTAATCGTTTTCAATATGACTACGTTCAAAAAAGAACATTTGATGTAGTAAGTTATCTTCATGAAGGCAATGATTTTAAAGTTTGAGACATTAACTTCCCTCTTTCAATTCGTGAAGAAAAGAAAGTTAGATTCAATGCCTACGTTCTTTTACATTACAAAAATGAAGGCAAAGTACTTTGATTCTATACAAACAAGAAGATTCAAAAAAAATTTGGTTTAAATGAAAAGGAAGCAAATGATGTGGGAATTTTAGCAAATATTGGTGATGCTAAAATTTGAGTATTATTCATTGATGAACCAAATGGCAAAATTAGAGCAAGAGTAAGAAGCAATGGAATATGAATAAATCATATTTGTGCAAAACCTGAATATTACCCTGGCGGTGGTCACGAAGTTGCTTCAGGAGCAACTTGTGCTAATAAAAAATCTATGAAACATTTAATAGAAGACTTAAAGGCAGAGGTATTAAAATATGAATAAAATAAGTGCAGAAAAATTAGCAATTTTCAAGAAAATTGAAGAAAGAATTAAAGCACACAAAAATATAGTTATATATCATCATATTAGACCTGACGGAGATTGCTTGGGTTCTCAATTTGGAATGAAGAATTTAATTAAGGTAAATTTCCCTGATAAAACAGTGTATACAATCGGTGATTCCAAAGGTATTTATTCATTTTTAGAATTTAAAATGGATAAATTACCACTAGAAAAGTTAGATGATTCATTAGCAATTATTGTTGATGCTAACTTTAAAGAACGTTTAGAATGTAGAGAATACTTGGATAATAATTGTTTTGATGAAGTAATAAGAATCGACCACCATCCAAATGATGATGATCTAGATGCTTCATTAAGATGAGTTGAACCAGAAGCACCAGCTGCTGCTCAACAAGTAACTGAAATAGCATACGAATTGGGTTGAAAATTAAACAAAGAAGCTGCAACTTATTTATATTTAGGTATATATACTGATTCAGTTAAATTGTCAACCAATACGACAACCGCAAAGACAATGTTGTTAGTAAGCTGATTGTGAGACAATGGTAGCCAAAAAGACTTAATTCATACAGAACTTTCTAAGCGTACATTGTTTGATATTAATATAAATACATATATATCTCAAAACATGAAAATTGCAAACGACGTCGTTTCATTTTACTTCCCATTGTCTGAACAAATTAAATTAGGAATTAAAGATCCATTAAAAGCAAATAGACCTTTTGTTCTAGGCTCAATTGACAATACAAGGGCATGAGTATTCTTTACAGAAGAAAAACCCGGCCAAATTCGTTGTGAATTTAGATCAAACGGTTGCAATGTAAGAAATGTAGCAATTAAATGAGGTGGGGGCGGACACATACGTGCATCAGGTGCTCAAATTTCTGATCCTAATTTAATTCCATTGATTATTAAAGATTTAGAACAAGAAACAAAAAACCTTGCTGATTATGAATAAAAAATAGAAATAATTTGTTAAAAAAGATGATTCTGTAAAATAAGAATCATCTTTTTTAAAATCTACCCTGAACGAAAACAAGGCTATGAGGATTTATCTAAACTAATTGAAGAGATAAATAATGGATTAAATTCTTTAGATATGAACGATATAAAGAATTTAGATTGAGTAAATGCAAAAAACTCAGAATTAAATAGAAAACATAATCATTATTTAAAAGATACAACCTTCTTACAGAAGAAAAAAAATAAAAGTTAAAAACCAAGATTTGACAATACAACAAATCTTGGTTTTTAACTAGAATTAAATGTTAGTTATGAATAAAATTATCCAATATATAATTATCAACTTTCTTGTTTATTGATTTAATAAAAGAAAAATAAGTTTTGTATTTCTTAAAATCTCCCTTTCAAACAAGCTTTTTATTTAATGAATTTGCTAAATCAATTGCCAAATAGACATCAAAGATTCCAAATTCATTATGGCAGTCAATAATCATTTTTTCAATTAATGAATTTATTTCGTGGTTTGAGATATCAATATTATCTCTTTTATATGAGCTTAATGCTTTAGTAAATAAATATCCAAAATATATGTTTCTACTTTGTTGATATTGTTTAAATTTTTCAATAAATTGCTTATGAATTTCATTTTCTTCAGGGCTTAGAGAAGCATTTGCAAAGAGCTTATCTGTATCAATTGCATTTTTTATATATACATTTTCTTTTTTTAATATTAATATCATTTTAATTACAATTAAAATAAATATAGCTAAGAAGATTGCGCCAAAAACATAACAAACAATACTTGATTTTTTAGATATAGTTCCCATAATGCCATTTGCTGCTAAAAATAGCAAGATAGCAATAACGAGAAAGAGAGCAACGGTTAGAAAACCAATAATGTTTAATATAACATTTTTTATTTTCATTTTCTTTTCTTTTGACGCCATTTATTTAACCTCCTTATAAGTTATTAGTTGAGGATTTTTTAAATATCCCCTTCCAGCTTTAGCAATATTATGGCCATTTAATCTTGTTGCATCGGCGCTAAAATTTACTCAAATTTTTAGCAACGCTGCACCAATGCCGTATGTGTCAACAGGAGTGTCTTCTGCTTCAAATTCAGCAATTCTTTGAGCGTCAAATCCACTTGAAACAGTTATTTTTACATGCTTGCCGTTATTATTGTCTAATGCTTCTCTTAATCTTTTTACTTGATTTGGTGTAACGCCAAATTCCGCTTCACCTTCTTTGAACATTTTATCAATTAGAGCTTTCGATGTGTCTATTCTTACACTTTTTAAATCTTTGCCAAAAGCCTTTAAACATTTTAATGAATCGCTTATAACATCGTTGTGAAAATCAACTAAGGCAACTAGATCATCATTTGGAAAAACTTTTTTATATGCTTTACAAGCAGCTACAATATCACCGTCAAACATTTGAATTAATGCATGAGGCATGCTTCCAAAAGTATGTTTTGGATCAAAATTTGAACTTAGAAAAGTCGAGTGATTTTCTATTCCACCTATTCTTATTGCATATGAGTCGTTTGGTTGATTTATATAATGATCTGCCCTATCTGCCATGCTTACGATTTTTTTGCCATTTGCTGCTTTTATTGCTCAATATGCATTAGTTGCTATTGATGATTGCCTTGCTAAAATGCCATCAATGATTCCTTCTCATATACCAAATTCATAATAAGGGCCTTCAAGTTCTAAAACTACTTCTCTATTGTGGATTATACTGCCTTCTGGCATGTATCTTATGGTGTATTTAGAAATATCTGTGTTTTGTTTTAAAATATCAATTGCTTCATTTATTCCACATAATTTGACATCATTATGTCTTTGAAAGAATTGCAAAGTAATGTTTTGATCTTTTTTAAACTTCTTTGATATTTTTTGAGTTTTGTAAAAATAAATTGAAATGTAATCTTTCAAATTGATTTTTTTCATTATTCTCCTTTTTATAATAATTTAATAAATTATTATTAGCCTCTTATTAAAATTTTAAACAAAATTAATTAATAAAATCATATAAAGTTATAAAATAATAAATCATTGTTAAAATATCTTTTTTAAAGACATGTTTTATAATAAAGGAGAATAAGTATGGCAAAAAACCAAGATAAAAAAATGCTATCTCCAGAAGAAAAAGAAATAGAAAATATAAAGAAAATATCAAAAAATGGTTGACATTTTAGTTATTGATCGTTATTTTTTATTTTAGGAGTTATAGCTATTTTAATATGAGCAGTTGAATCTAAAAGTTCATCTTCAATGGTAAAATATCCATTATCTATAATATTTATAATTCTTTGCATAGCAATGATAGCAGGAATGTATACAATAAATGTAATGGGTTATAAACTAGCAATCAAATTAAACAATGAATCTTTAAGAAAATGCTATTTGATTTCACTAATTCCAATATTGTTTTTTGTAGCATTAGTAGCAAGCTTTGTGTCTTCAATTAAGCTAAAAGAAATTTATATAGATAAAAAAAGCCAATTTCAAAACGTTGCTATAGCAGGATAGAAAAAAACCTTGAATTAATTTTCAAGGTTTTTTTGTTTAATTTCTTTTGCTATTTTGTTTGCATTTCTTTGGCGATGAGTAAATAGAATTATATAAAATACTAATGTAGGAATGGCAATGCTTATAAAGATTATAAAAAAGCAAGATAAGGCTATGCAACCATTTTGATTTCATAGGATAAAATAATTATCTAATGAAGTTATAGCATAAACACTTAATGTTGGAATAAACGCTATTGCAACTTGTGAAATTAAAGTGTGGCGCTTAGTTATTAATTGTGGTTTTTTAATTAACAGTATTGATGTTGTTAATATGTATGTAATTAAAATATAACCAACAATTACAAGTATTATAGGTAAGGTAATTTTCATAGTTAAAATTTTACTCCATTAAAAAATAAGAGCATTTAAAAAGCGCATGTTTTAAACATGGCTTTTTAAAATTAGTTATT", "length": 780024, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_013367855.1|CP055151.1", "orig_description": "HMT-632 Metamycoplasma hominis FBG" } ], "run": { "start": "2026-01-28 09:21:24", "end": "2026-01-28 09:29:16", "duration": "7.85 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }