{ "genome": { "genus": "Metamycoplasma", "species": "hominis", "strain": "SS25", "taxon": "Metamycoplasma hominis SS25", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 672843, "gc": 0.27034389894819444, "n_ratio": 0.0, "n50": 672843, "n90": 672843, "coding_ratio": 0.7422652832830244 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 472, "stop": 621, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTCAAGCAAAAATGGAAGAATTCAACCAAAAGAAAGCCAAAATTGAATCTAAATATGCTGCTTACAAAGGCGACAGACAAATGATGCAACGTAAGCAAGCTGAAATTAGTGAATTATATAAGAAAGAAAAAATTTCTCCTTGA", "aa": "MFSQAKMEEFNQKKAKIESKYAAYKGDRQMMQRKQAEISELYKKEKISP", "aa_hexdigest": "7588ca6ca96a15cae5b57833b385386d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5875.7765, "isoelectric_point": 9.646807289123537 }, "id": "AHCKFKLKGB_43", "locus": "GCA013367975_00001" }, { "type": "cds", "sequence": "contig_1", "start": 850, "stop": 1005, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTTCAACTATTTACTTTTGTTAAAAGTGTTTTCCAACAATACTTATCAAATGTAAATTATGTAAAATTACCTTCTAAAACAAATGACGGTAGTTTTGTATATAGAAAATCAAGTCTAAGTCCTGCTTCAAATTACAATTCAGAATATTGA", "aa": "MLLQLFTFVKSVFQQYLSNVNYVKLPSKTNDGSFVYRKSSLSPASNYNSEY", "aa_hexdigest": "70e3238dbe95673daecbc9a241e2394a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5904.613100000001, "isoelectric_point": 9.456109809875489 }, "id": "AHCKFKLKGB_44", "locus": "GCA013367975_00002" }, { "type": "cds", "sequence": "contig_1", "start": 1300, "stop": 1434, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGACGAAAAAGGAAATTACAAAACCAAAAATTACACTCCATTAACCAAATTCAGTGAAATTCTAATTCCTAATTCTTTATCGTTTGACACTAAGCCAAAAGATAAAGATGGCATTATACCAACAGATTGA", "aa": "MGDEKGNYKTKNYTPLTKFSEILIPNSLSFDTKPKDKDGIIPTD", "aa_hexdigest": "00a76826a52447d9c1fa25fe72451b42", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4961.553500000002, "isoelectric_point": 6.104928398132324 }, "id": "AHCKFKLKGB_45", "locus": "GCA013367975_00003" }, { "type": "cds", "sequence": "contig_1", "start": 1513, "stop": 1854, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTATCGGTTTAGTTGGTTGTGTACAATCATTTACTGTAAAATCAAGTAATAAAGTCGGTTCTGGACAAGAGCTATATTCTAAAAAGAGTGACATTTCACCAAATATTGTCACTTATAGATACGATAGTACTGGTAATTTCTTTACCTTACCAAACAAAATAAATGATAAAAATATTATCGCCAATACATATCTTGCTTTAAAAGAACCTAATGCTATTAATGAGTTAAGGAAAAGAGATCCTAATTATGGCGCATATGGTTCTCGTTCATTTGGGTTGCAATTGCAAATGCCAAAAAACGACAAAGCAAAAGTTTTATCTTCTCATGATGAAGATTGA", "aa": "MAIGLVGCVQSFTVKSSNKVGSGQELYSKKSDISPNIVTYRYDSTGNFFTLPNKINDKNIIANTYLALKEPNAINELRKRDPNYGAYGSRSFGLQLQMPKNDKAKVLSSHDED", "aa_hexdigest": "200f4ab9d28dac35760f2bb7d475a211", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12536.983699999986, "isoelectric_point": 9.211774635314942 }, "id": "AHCKFKLKGB_46", "locus": "GCA013367975_00004" }, { "type": "cds", "sequence": "contig_1", "start": 1970, "stop": 2299, "strand": "-", "frame": 3, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "COG:COG0594", "COG:J", "EC:3.1.26.5", "GO:0000049", "GO:0004526", "GO:0008033", "RefSeq:WP_012855283.1", "SO:0001217", "UniParc:UPI00006920BE", "UniRef:UniRef100_D1J7D9", "UniRef:UniRef50_A0A448ZZD2", "UniRef:UniRef90_D1J7D9" ], "nt": "ATGATAAATAAAAATAACGTTGTTAAAAAAAATTGGGAATTTCAGAAAATTCTTGATTCTCACAATCAAGTGGTTTCAAAAAATTTAATAATTTATTTTAACAAATCCGATTTCTTTAAAGTGGGTATTTCAATCCCTAAGCAATTTGCAAATGCTGTTTTGAGAAATCATTACAAAAATCAAATCAGATCAATTCTAAGAAACATTGATTTTAGCACTTTAAATTACGAAACTGTGATTATTGCTCGCAAAGGCTTCTTGAGTCTTGACTGGAATCAAAAAGTTAAAGAAGTAAAAAAACTTTATGAAAGGATCGCTGATGGCAAATAA", "aa": "MINKNNVVKKNWEFQKILDSHNQVVSKNLIIYFNKSDFFKVGISIPKQFANAVLRNHYKNQIRSILRNIDFSTLNYETVIIARKGFLSLDWNQKVKEVKKLYERIADGK", "aa_hexdigest": "ecba321b6721ed7a45d96e43c86447b3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00006920BE", "ncbi_nrp_id": "WP_012855283.1", "uniref100_id": "UniRef100_D1J7D9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00006920BE", "RefSeq:WP_012855283.1", "UniRef:UniRef100_D1J7D9" ] }, "ips": { "uniref100_id": "UniRef100_D1J7D9", "uniref90_id": "UniRef90_D1J7D9" }, "psc": { "uniref90_id": "UniRef90_D1J7D9", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_A0A448ZZD2", "cog_id": "COG0594", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0004526", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZD2" ], "product": "Ribonuclease P protein component" }, "genes": [ "rnpA" ], "id": "AHCKFKLKGB_47", "locus": "GCA013367975_00005" }, { "type": "cds", "sequence": "contig_1", "start": 2327, "stop": 2473, "strand": "-", "frame": 3, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "KEGG:K02914", "RefSeq:WP_012855284.1", "SO:0001217", "UniParc:UPI00006920BF", "UniRef:UniRef100_D1J7E0", "UniRef:UniRef50_P05647", "UniRef:UniRef90_B3PNJ7" ], "nt": "ATGAAAAGAACATACCAACCAAAAAAACGCAAACACATTAAAGTTCATGGTTTTATGGCTAGAATGCAAACTAAAAATGGTAGAAAAGTTTTAGCAGCTAGACGTGCAAAAGGTAGATATACATTAACTGTATCTGACAATAAATAA", "aa": "MKRTYQPKKRKHIKVHGFMARMQTKNGRKVLAARRAKGRYTLTVSDNK", "aa_hexdigest": "c12d043f3061d758a33db3c492b0b7ea", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00006920BF", "ncbi_nrp_id": "WP_012855284.1", "uniref100_id": "UniRef100_D1J7E0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00006920BF", "RefSeq:WP_012855284.1", "UniRef:UniRef100_D1J7E0" ] }, "ips": { "uniref100_id": "UniRef100_D1J7E0", "uniref90_id": "UniRef90_B3PNJ7" }, "psc": { "uniref90_id": "UniRef90_B3PNJ7", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_P05647", "cog_id": "COG0230", "cog_category": "J", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P05647", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05647" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "AHCKFKLKGB_48", "locus": "GCA013367975_00006" }, { "type": "oriC", "sequence": "contig_1", "start": 2474, "stop": 2805, "strand": "?", "product": "origin of replication", "nt": "AACAATTCTCCTTTGCGTTTTTATATATAAAATAACTTAATTTATTTTACCAAAAATACGACAAAAAAAGCACATAAAATACCTAAGAAAAAAAATTTAATATTTTTTCTGTTTTTAATATACATTAAAAGAAACATAAAATTCAAAGTTAATAACAAAATAAACAAAGAAATTATTTAAATGTTTATTATGTTATTTTAGTATAAAAAACCTCTTATTTTAAAAACAAAAGGCCTAAAAAAAATGTTAATAATTTTGTTAATAATTTGTAAATTAGCATTTATAAGGGCTTATTAATTTATTAATTTTGAATTTTAAAATATAATAGAGGT", "id": "AHCKFKLKGB_1155" }, { "type": "cds", "sequence": "contig_1", "start": 2806, "stop": 3735, "strand": "+", "frame": 1, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "KEGG:K02313", "SO:0001217", "UniRef:UniRef50_A0A448ZWT6", "UniRef:UniRef90_D1J7E1" ], "nt": "ATGAAAACTACAAATCCCTCTGAATTAGATTTAAAAGCGCATAATATGTCATTTCAAAAAGAATTAGTTAATACTGCAAGTGATCCCTTAATATATGATCAATTCTTGTCGTCATTAGAAATTGTAAAAGAAAATAATAAAATGATTTACATATTAGTTAAAGGACCTGAAGATGTTTTAACATATATTAAGAACAGTCATAATGAAAGTATTAAAAGAGCAATTAAAAACGTTTATGGTGAAGAATATAATTTGGTTTATATTAGAAATTTAGATGAAATTAAAAGCTTGCAAGATAGCAATAATGTTGAATCTCCAACTAATTCATTGGCTAATAATTCTAAAGAAAATTTTAGCAATATTAAGAAGAATTTAACATTTGATGATTATGCAATAGGAAAATTTAATAATATGGCTTTAAAGGCAGCAAAGGCTATTTGCAATAGTGAAAAGATATTGTTCTCTCCTTTATTTATTCATGCTTCAAGTGGGCTTGGTAAAACGCATCTATTACATGCTATTGGAAATGAATTATTAAAACACGGTAGAAGTGCATTATATATAAATCCTGATTCATTGACAAGAAGATTAGTTGAACAATTAAAATCAAAGAATCAAGAACAAATTAATAAAATTATTGATGAATTAATGTCTTATGATTGTCTAATGTTTGATGATGTTCAACAATATGGTAATCGTGATTCTACATTAAATGTCTTATTTAATATTATTGATAATATGATAATGAACGATAAGCAAATAATCTTTTGTGGTGATAAAAAGCCGGATGATTTAGGTGGATTTGAACAAAGATTTATAACTAGATTTAATGGCGGATTAACTGTTGAAATTTTAAAACCAGAGTTAAATGATGTAATTAATGTCTTGAAATTTAAATTGCAAGAAAATGGTATAAATCCGGAACTTTGA", "aa": "MKTTNPSELDLKAHNMSFQKELVNTASDPLIYDQFLSSLEIVKENNKMIYILVKGPEDVLTYIKNSHNESIKRAIKNVYGEEYNLVYIRNLDEIKSLQDSNNVESPTNSLANNSKENFSNIKKNLTFDDYAIGKFNNMALKAAKAICNSEKILFSPLFIHASSGLGKTHLLHAIGNELLKHGRSALYINPDSLTRRLVEQLKSKNQEQINKIIDELMSYDCLMFDDVQQYGNRDSTLNVLFNIIDNMIMNDKQIIFCGDKKPDDLGGFEQRFITRFNGGLTVEILKPELNDVINVLKFKLQENGINPEL", "aa_hexdigest": "5cf4c206747ecab97980db0ee426889e", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaA" ], "truncated": "3-prime", "pseudogene": { "start": 2806, "stop": 4038, "observations": { "mutation": [ 3733 ], "stop": [ 3733 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E1", "query_cov": 1.0, "subject_cov": 0.6673866090712743, "identity": 0.987, "score": 599.0, "evalue": 9.56e-214, "gene_start": 1, "gene_end": 309, "reference_start": 1, "reference_end": 309, "reference_sequence": "MKTTNPSELDLRTHNMSFQKELVNTASDPLIYDQFLSSLEIVKENNKMIYILVKGPEDVLTYIKNSHNESIKRAIKNVYGEEYNLVYIRNLDEIKSLQDSNNVESATNSLANNSKENFSNIKKNLTFDDYAIGKFNNMALKAAKAICNSEKILFSPLFIHASSGLGKTHLLHAIGNELLKHGRSALYINPDSLTRRLVEQLKSKNQEQINKIIDELMSYDCLMFDDVQQYGNRDSTLNVLFNIIDNMIMNDKQIIFCGDKKPDDLGGFEQRFITRFNGGLTVEILKPELNDVINILKFKLQENGINPELWEEESLKFIARNFSSSIRNIEGAINRIKLFQDGDDGFFTYDINTIKSIFNSMTQVAENITPDKIIDAVSKYYKIDRKKILSNVRTNDVVNARRIATYLVNKNFNYTLQEIGKVVGNQSHSNVIVSLNWIEKNANSNPTLKLALQKIESNLKKIS" }, "paralog": false, "identity": 0.9878048780487805, "score": 799.0, "evalue": 3.39e-295, "description": "Internal stop codon at 3733. Nonsense mutation around 3733.", "psc": { "uniref90_id": "UniRef90_D1J7E1", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_A0A448ZWT6", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E1", "UniRef:UniRef50_A0A448ZWT6", "COG:COG0593", "COG:L", "KEGG:K02313" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZWT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZWT6" ], "product": "Chromosomal replication initiator protein DnaA" } }, "id": "AHCKFKLKGB_49", "locus": "GCA013367975_00007" }, { "type": "cds", "sequence": "contig_1", "start": 3886, "stop": 4116, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08299.17" ], "nt": "ATGACTCAAGTTGCTGAGAATATTACCCCCGATAAAATAATTGATGCTGTTTCTAAATACTATAAAATTGACAGAAAAAAAATATTGAGTAATGTAAGAACAAATGACGTGGTTAATGCCCGCAGAATAGCAACATATTTAGTAAATAAAAATTTTAATTATACATTGCAAGAAATAGGCAAGGTTGTAGGAAATCAATCACATTCAAACGTTATTGTTTCACTTAATTGA", "aa": "MTQVAENITPDKIIDAVSKYYKIDRKKILSNVRTNDVVNARRIATYLVNKNFNYTLQEIGKVVGNQSHSNVIVSLN", "aa_hexdigest": "28ae5fe87d266549f489ee290f7117cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08299.17", "name": "Bac_DnaA_C", "length": 66, "aa_cov": 0.868421052631579, "hmm_cov": 1.0, "evalue": 2.1841906933005715e-21, "score": 71.04753875732422, "start": 10, "stop": 75 } ], "seq_stats": { "molecular_weight": 8637.7939, "isoelectric_point": 9.753437995910645 }, "id": "AHCKFKLKGB_50", "locus": "GCA013367975_00008" }, { "type": "cds", "sequence": "contig_1", "start": 4213, "stop": 4611, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00712.24" ], "nt": "ATGAAATTAAAAATTGATAAATTATTATTAGATAATGCAATTGAAAGAGTATCAAAATCTGTGGATGCTAATCCATTTTTACCAGTCATGAAAGGTATTTTAATTCAAGCAGAAGATAACAAAATAACATTAATTGGTTCAAATGGTGAAACAAGCATAAAGCATGAAATAGAAACATCGATGGATGCCCAAATTGAATTGCCCGGAATTGTATTAGTTGAACTTAGCTTATTTAAAAATATTATTAAAAAGTTAGATGGCGAAATAGTATTAGAAGTTGTAAATAATATTCTTAAAATAAGTACAAACAACGAAAATTATAATTTAAATATATATGATGTAAATGAATATATTGCAATTGATTTTGCATGTTATGGAGAACAAGTAAAAATTAACTGA", "aa": "MKLKIDKLLLDNAIERVSKSVDANPFLPVMKGILIQAEDNKITLIGSNGETSIKHEIETSMDAQIELPGIVLVELSLFKNIIKKLDGEIVLEVVNNILKISTNNENYNLNIYDVNEYIAIDFACYGEQVKIN", "aa_hexdigest": "9ef52e86224a71ce7e6a51915943094c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00712.24", "name": "DNA_pol3_beta", "length": 122, "aa_cov": 0.9090909090909091, "hmm_cov": 0.9754098360655737, "evalue": 2.4921319227061984e-18, "score": 61.724979400634766, "start": 1, "stop": 120 } ], "seq_stats": { "molecular_weight": 14804.990099999985, "isoelectric_point": 4.6782129287719725 }, "id": "AHCKFKLKGB_51", "locus": "GCA013367975_00009" }, { "type": "cds", "sequence": "contig_1", "start": 4678, "stop": 5310, "strand": "+", "frame": 1, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360", "KEGG:K02338", "SO:0001217", "UniRef:UniRef50_A0A2Z4LLI0", "UniRef:UniRef90_D1J7E2" ], "nt": "TTGTGCTGTGTCAATATATCGGCAGCCAATAAAAAAATAAAGTTTATAACAACAGACAGATTTAGATATGCCCAAGCAGTTCAAGACATTTCTGAAGATGTAACATTCAATATTTCAATATTAGCTAAAAACTTAAGAGATATTACCAACTTTGATTACAATGGCGACGTTATTTTAAATATTTCTGATCAAAAGATTTCATTTAAATTAGACTCAACAACTATTGAATCAAAAGTTGTTGATCAATTGTATCAAGATGTTTCAAGAATTGTTCCACAAGAATTTGACACCGAACTTAAAATAACTAAAAAAGAACTAAATAACTTGTTAAATAAGGCATCAGTTATTATTAATGAAAATTATAATAAAATTAAGTTGCACATTGTTGATAATGTTTTAACCATTTCATCTGCTAGAGAAGAAATTGCTAATGCTGAAATTAAAACAAATAATTTTTCTTACAACGGTGAAGAATTGAAACTCGCATTAAATAGTAGATTCTTAAAGGATGCTATTTCTGTATATGAAGATAATTTGAATATTTTATTTACAAAAGATAAATTAAGAATAGTCATAAAATCAGATTCTAACCCAAATGTGCTACAATTGATTACGCCACAAAAAGGATTCTAA", "aa": "MCCVNISAANKKIKFITTDRFRYAQAVQDISEDVTFNISILAKNLRDITNFDYNGDVILNISDQKISFKLDSTTIESKVVDQLYQDVSRIVPQEFDTELKITKKELNNLLNKASVIINENYNKIKLHIVDNVLTISSAREEIANAEIKTNNFSYNGEELKLALNSRFLKDAISVYEDNLNILFTKDKLRIVIKSDSNPNVLQLITPQKGF", "aa_hexdigest": "5fd6dec55127547b14c9da8b30a299ed", "start_type": "TTG", "rbs_motif": null, "genes": [ "dnaN" ], "truncated": "5-prime", "pseudogene": { "start": 4378, "stop": 5310, "observations": { "mutation": [ 4609 ], "stop": [ 4609 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E2", "query_cov": 1.0, "subject_cov": 0.5753424657534246, "identity": 0.995, "score": 399.0, "evalue": 5.1e-138, "gene_start": 1, "gene_end": 210, "reference_start": 156, "reference_end": 365, "reference_sequence": "MKLKIDKLLLDNAIERVSKSVDANPFLPVMKGILIQAEDNKITLIGSNGETSIKHEIETSIDAQIELPGIVLVELSLFKNIIKKLDGEIVLEVVNNILKISTNNENYNLNIYDVNEYIAIDFACYGEQVKINWNDFKSLVRDVIFAASTNEVSLILCCVNISAANKKIKFITTDRFRYAQAVQDISEDVTFNISILAKNLRDITNFDYNGDVILNISDQKISFKLDSTTIESKVVDQLYQDVSRIVPQEFDTELKITKKELNNLLNKASVIINENYNKIKLHIVDNVLTISSAREEIANAEIKTNNFSYNGEELKLALNSRFLKDAISVYEDNLNILFTKDKLRIVIKSDSNPNVLQLITPQKGF" }, "paralog": false, "identity": 0.9935483870967742, "score": 592.0, "evalue": 2.12e-216, "description": "Internal stop codon at 4609. Nonsense mutation around 4609.", "psc": { "uniref90_id": "UniRef90_D1J7E2", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A2Z4LLI0", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E2", "EC:2.7.7.7", "UniRef:UniRef50_A0A2Z4LLI0", "COG:COG0592", "COG:L", "KEGG:K02338", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z4LLI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z4LLI0" ], "product": "DNA polymerase III subunit beta" } }, "id": "AHCKFKLKGB_52", "locus": "GCA013367975_00010" }, { "type": "cds", "sequence": "contig_1", "start": 5310, "stop": 5483, "strand": "+", "frame": 3, "gene": "ybcJ", "product": "Ribosome-associated protein YbcJ, S4-like RNA-binding protein", "db_xrefs": [ "COG:COG2501", "COG:J", "SO:0001217", "UniRef:UniRef50_N9UZE0", "UniRef:UniRef90_D1J7E3" ], "nt": "ATGGAAATAGAAATTACTGGAGAATTTATTAAATTAAGTCAATTTTTAAAGAAAATTGATGTGTGCCCAACGGGCGGAATGGCAAAATATTTTGTTCAGGTTCACAGAATATTGATTAACGGGAACCTTGCAAACGGAAGAAATGCTAAGATTTATCCCGGCGATACCGTTTGA", "aa": "MEIEITGEFIKLSQFLKKIDVCPTGGMAKYFVQVHRILINGNLANGRNAKIYPGDTV", "aa_hexdigest": "c366bbf7c6e153c1c5ccd4e3f3610b33", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7E3", "query_cov": 1.0, "subject_cov": 0.8142857142857143, "identity": 1.0, "score": 119.0, "evalue": 4.16e-34, "valid": true, "gene": "YbcJ", "product": "Ribosome-associated protein YbcJ, S4-like RNA-binding protein", "uniref50_id": "UniRef50_N9UZE0", "cog_id": "COG2501", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_N9UZE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UZE0" ], "product": "RNA-binding S4 domain-containing protein" }, "genes": [ "ybcJ" ], "id": "AHCKFKLKGB_53", "locus": "GCA013367975_00011" }, { "type": "cds", "sequence": "contig_1", "start": 5591, "stop": 5788, "strand": "+", "frame": 2, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "COG:COG0227", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_012855288.1", "SO:0001217", "UniParc:UPI0001BC1FC5", "UniRef:UniRef100_D1J7E4", "UniRef:UniRef50_Q98PM6", "UniRef:UniRef90_B3PLS0" ], "nt": "ATGCCAGGAAGAGATCAATTAACAGGTCAAAAAGCTCTAAGTGGAAACAAAAGATCACACGCTTTAAACACCGCAAAAAGAACATTTGACTTAAATCTTCAAAAAGTTACTGTTTTAACTGAAACCGGAAGTAAAAAAACAGTTCGTGTAACTGCAAAAAATGCTAGAACACTAAAAAAATATGGTCTAGTTGCATAG", "aa": "MPGRDQLTGQKALSGNKRSHALNTAKRTFDLNLQKVTVLTETGSKKTVRVTAKNARTLKKYGLVA", "aa_hexdigest": "be79e6e221684802b48e19dfde4c6d71", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC1FC5", "ncbi_nrp_id": "WP_012855288.1", "uniref100_id": "UniRef100_D1J7E4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC1FC5", "RefSeq:WP_012855288.1", "UniRef:UniRef100_D1J7E4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7E4", "uniref90_id": "UniRef90_B3PLS0" }, "psc": { "uniref90_id": "UniRef90_B3PLS0", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q98PM6", "cog_id": "COG0227", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PM6" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "AHCKFKLKGB_54", "locus": "GCA013367975_00012" }, { "type": "cds", "sequence": "contig_1", "start": 5858, "stop": 6025, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCAATACAAACGTAAAACTCTATTAAATAATTTTCCAAGCAATTTAAAACAAAAAATTTCTGAATTTTTAAAAAGCAATGAACTAGATTTAAATATAAGAGCTGAAAAAATTACAAAAGAGATTTCTATTAATTTATTTAAATTTATAAACAATAAAAATTAA", "aa": "MFQYKRKTLLNNFPSNLKQKISEFLKSNELDLNIRAEKITKEISINLFKFINNKN", "aa_hexdigest": "d061f8f47610aed152b4b48fc3d66d05", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6618.659699999998, "isoelectric_point": 9.996741676330569 }, "id": "AHCKFKLKGB_55", "locus": "GCA013367975_00013" }, { "type": "cds", "sequence": "contig_1", "start": 6062, "stop": 6631, "strand": "-", "frame": 3, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "COG:COG0030", "COG:J", "EC:2.1.1.182", "GO:0000179", "GO:0006364", "SO:0001217", "UniRef:UniRef50_D1J7E5", "UniRef:UniRef90_D1J7E5" ], "nt": "ATGATTAAAGCATTGAAATCACTAGGCCAAAATTTCTTAAGCAATAAAAACATTCAAAAAGAGATTGTAAAGGCTGCGAACGTTGCAGGCAAAAATATTATTGAAATTGGCCCGGGAATGGGCGCAATAACTGATCAAATGGCGGATGTTGTAAATAGACTTGTATGTATTGAATTTGATAAAAGATTATACGAATTTTTATTACAAAAAAATTATTCAAGCAATGTTGAAATATTGAACCAGGATTTCTTACAAACTGATTTATTAAAATATGCTGATTTTGAAGTAATTGGAAACATTCCATATAATATAACAAGCGATATAATTTTTAAATTATTAGATAATGCTAAAAACATTAATAAAATAACATTAATGGTTCAAAAAGAAGTAGCAGATAGAATATGCTGTGGAGCAGGTAATTCTCAATATAGTAAATTAGCAGCATCAATACAACTTCTCTATGAACCTAAATATTTATTCACTGTTAAGGCAAAAGAATTTAATCCCGCTCCTAAAGTTGATTCGGCAGTTATTCAACTAAATCTAATAAAAAATAATGATTTTATTTGA", "aa": "MIKALKSLGQNFLSNKNIQKEIVKAANVAGKNIIEIGPGMGAITDQMADVVNRLVCIEFDKRLYEFLLQKNYSSNVEILNQDFLQTDLLKYADFEVIGNIPYNITSDIIFKLLDNAKNINKITLMVQKEVADRICCGAGNSQYSKLAASIQLLYEPKYLFTVKAKEFNPAPKVDSAVIQLNLIKNNDFI", "aa_hexdigest": "40356ed03a40dca308d55e1bdd56440a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rsmA" ], "truncated": "5-prime", "pseudogene": { "start": 5858, "stop": 6655, "observations": { "mutation": [ 6064 ], "stop": [ 6064 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E5", "query_cov": 1.0, "subject_cov": 0.7132075471698113, "identity": 0.9840000000000001, "score": 364.0, "evalue": 3.37e-126, "gene_start": 1, "gene_end": 189, "reference_start": 9, "reference_end": 197, "reference_sequence": "MLERFLKSMIKALKSLGQNFLSNKNIQKEIVKAANVAGKNIIEIGPGMGAITDQMADVVNRLVCIEFDKRLYEFLLQKNYSSNVEILNQDFLQTDLLKYTDFEVIGNIPYNITSDIIFKLLDNAENINKITLMVQKEVADRICCGAGNSQYSKLAASIQLLYEPKYLFTVKAKEFNPAPKVDSAVIQLILIKNNDFIWNNQVEILKFIKQMFQYKRKTLLNNFPSNLKQKISEFLKSNELDLNIRAEKITKEISINLFKFINNKN" }, "paralog": false, "identity": 0.9849056603773585, "score": 506.0, "evalue": 1.48e-184, "description": "Internal stop codon at 6064. Nonsense mutation around 6064.", "psc": { "uniref90_id": "UniRef90_D1J7E5", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_D1J7E5", "cog_id": "COG0030", "cog_category": "J", "go_ids": [ "GO:0000179", "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E5", "EC:2.1.1.182", "UniRef:UniRef50_D1J7E5", "COG:COG0030", "COG:J", "GO:0000179", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7E5" ], "product": "Ribosomal RNA small subunit methyltransferase A" } }, "id": "AHCKFKLKGB_56", "locus": "GCA013367975_00014" }, { "type": "cds", "sequence": "contig_1", "start": 6624, "stop": 7304, "strand": "-", "frame": 2, "gene": "tatD", "product": "YchF/TatD family DNA exonuclease", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.21.-", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_N9U095", "UniRef:UniRef90_A0A6A8Q279" ], "nt": "ATGGAAAAACTTTTTGTATGTGGAACAAACGAAGATGATTCGTTGCAAGCAATAGAACTTGCTAAAAAGGTTGATTTTGTTTATCCTGTTATTGGAATACATCCAACATTATCAAATGGTTTGCAAGATAGAGAAATTTTAAACAAGATATATGAACCAAATGTTGTTGCAATTGGAGAAGTTGGCCTTGATTATCATTATGATGATTCGCCTAGCAAAGAAATTCAAAAACAAGGATTTATTGCTCAAATAGATTTTGCAAAAGAACATAATTTGCCAGTAATTATGCACATTAGAGATGCTTTAGATGATTCTTTAGATATAATAACCTTGCCTCAATATAAAGACATTAATTTTATATTTCACTCATTTAGTGGGGATCTTACTTTTTTAAATAAATGCTTGACATATTCAAATATTTATTATTCAATAAGCGGGGTTGTTACTTTTAAAAATGCTAAGCCTTTACAAGAAGCAGTAAAAAATATTCCTTTAGATAGAATATTTTGTGAGACCGATACACCTTATTTAGCTCCAACTCCAATGAGAGGAAAACCCAACATTAGTCCTTTTGTTCAATACACATATGAATATATTGCTAATTTAAAACAAATTGATGTTGATGTTTTAGTCAAACAAATTAAAGAGAATGTTAGAAAGGTTTTTAAAATCTATGATTAA", "aa": "MEKLFVCGTNEDDSLQAIELAKKVDFVYPVIGIHPTLSNGLQDREILNKIYEPNVVAIGEVGLDYHYDDSPSKEIQKQGFIAQIDFAKEHNLPVIMHIRDALDDSLDIITLPQYKDINFIFHSFSGDLTFLNKCLTYSNIYYSISGVVTFKNAKPLQEAVKNIPLDRIFCETDTPYLAPTPMRGKPNISPFVQYTYEYIANLKQIDVDVLVKQIKENVRKVFKIYD", "aa_hexdigest": "d4ad969ed92c07b5e2570d6d24ce6015", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A6A8Q279", "query_cov": 1.0, "subject_cov": 0.8862745098039215, "identity": 0.9690000000000001, "score": 448.0, "evalue": 9.16e-159, "valid": true, "gene": "TatD", "product": "YchF/TatD family DNA exonuclease", "ec_ids": [ "3.1.21.-" ], "uniref50_id": "UniRef50_N9U095", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424" }, "pscc": { "uniref50_id": "UniRef50_N9U095", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U095" ], "product": "TatD DNase family protein" }, "genes": [ "tatD" ], "id": "AHCKFKLKGB_57", "locus": "GCA013367975_00015" }, { "type": "cds", "sequence": "contig_1", "start": 7391, "stop": 7564, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATGTTGTTCATTGGCACATACATCATTTATTTTTGGCAAACCATTTTATTCATTCAAGGTAATTTCGGATTGTTTGGAATCAAATAATAACCTAGAATATAGGCAATCAAAAGCAATTTGGGAATTAAAAATAATTGATATTTTTAAAGATATAATAGGAGAATTATAA", "aa": "MECCSLAHTSFIFGKPFYSFKVISDCLESNNNLEYRQSKAIWELKIIDIFKDIIGEL", "aa_hexdigest": "e3ee504ddb9e8f5b5d171cb39f314c82", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6649.642099999997, "isoelectric_point": 5.122978019714354 }, "id": "AHCKFKLKGB_58", "locus": "GCA013367975_00016" }, { "type": "cds", "sequence": "contig_1", "start": 7589, "stop": 7924, "strand": "-", "frame": 3, "gene": "mtnN", "product": "Nucleoside phosphorylase domain-containing protein", "db_xrefs": [ "COG:COG0775", "COG:FH", "SO:0001217", "UniRef:UniRef50_A0A454C9F7", "UniRef:UniRef90_A0A454C9F7" ], "nt": "TTGTTAGCGCACTGCGGAGTTGGAAAAACTAATTCTGCTTTAATGTTAAGTTATTTATTGACTAAATTCAATATATTAAAAGTAATTAACATTGGCCCTGCTATATCAATTTTTAATGGCTGTGTTGGAGATATTTATTGCATACAAAACTGCAAATATTATGATGTTAATTTAACCGCCTTGCCTAATTATAAAATAGGGCAATTGCCTAATTTGCAAGATGTATTTTATTGCAATCAAGATCTAAAAATTTGCAGCAATTTTAAAAAAGCCAATATTATAACCGGTGATAGTTTTATTAATAAAAGCATAATAAATTCAAATATATTAGATTGA", "aa": "MLAHCGVGKTNSALMLSYLLTKFNILKVINIGPAISIFNGCVGDIYCIQNCKYYDVNLTALPNYKIGQLPNLQDVFYCNQDLKICSNFKKANIITGDSFINKSIINSNILD", "aa_hexdigest": "518a116f577de6940300b1cc102f1e67", "start_type": "TTG", "rbs_motif": null, "genes": [ "mtnN" ], "truncated": "both", "pseudogene": { "start": 7391, "stop": 8077, "observations": { "mutation": [ 7591, 7942 ], "stop": [ 7591, 7942 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9F7", "query_cov": 1.0, "subject_cov": 0.4868421052631579, "identity": 0.955, "score": 218.0, "evalue": 2.72e-70, "gene_start": 1, "gene_end": 111, "reference_start": 52, "reference_end": 162, "reference_sequence": "MEKKFYLFIFAEPLETEQLRIKYSKHLKQIKIDFENNFQNLYELNWNNKQILLAHCGVGKTNSALMLSYLLTKFNILKVINIGPAISIFNGSVGDIYCIQNCKYYDVNLTALPNYKIGQLPSLQDVFHCNQDLKICSNFKKANIITGDSFINKSIINSDILDWNTNNLLGDMECCSLAHTSFIFGKPFYSFKVISDCLESNNNLEYRQSKAIWELKIIDIFKDILGEL" }, "paralog": false, "identity": 0.956140350877193, "score": 444.0, "evalue": 8.85e-162, "description": "Internal stop codon at 7591, 7942. Nonsense mutation around 7591, 7942.", "psc": { "uniref90_id": "UniRef90_A0A454C9F7", "gene": "MtnN", "product": "Nucleoside phosphorylase domain-containing protein", "uniref50_id": "UniRef50_A0A454C9F7", "cog_id": "COG0775", "cog_category": "FH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9F7", "UniRef:UniRef50_A0A454C9F7", "COG:COG0775", "COG:FH" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9F7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F7" ], "product": "Nucleoside phosphorylase domain-containing protein" } }, "id": "AHCKFKLKGB_59", "locus": "GCA013367975_00017" }, { "type": "cds", "sequence": "contig_1", "start": 7940, "stop": 8077, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAGAAATTTTATTTATTTATTTTTGCTGAACCACTAGAAACTGAACAATTAAGACTAAAATGTAGCAAGTATTTAAAACAAATAAAAATTGATTTTGAAAATAATTTTCAAAATCTTTATGAATTAAATTGA", "aa": "MEKKFYLFIFAEPLETEQLRLKCSKYLKQIKIDFENNFQNLYELN", "aa_hexdigest": "fb77b0c0ec8dcfe57ae641c47ae7faa0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5621.481999999999, "isoelectric_point": 6.152388954162597 }, "id": "AHCKFKLKGB_60", "locus": "GCA013367975_00018" }, { "type": "tRNA", "sequence": "contig_1", "start": 9161, "stop": 9247, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 74.5, "nt": "GCCCAGGTGGCGGAATGGTAGACGCTACGGACTTAAAATCCGTTGGCTGTAATGGCCGTGCTGGTTCAAGTCCAGTCCTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 9194, 9196 ], "id": "AHCKFKLKGB_1", "locus": "GCA013367975_00019" }, { "type": "tRNA", "sequence": "contig_1", "start": 9253, "stop": 9328, "strand": "+", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 80.1, "nt": "GCGCCCTTAGCTCAGCAGGTAGAGCAAATGGCTTTTAACCATTGGGTCAGAGGTTCGAATCCTCTAGGGCGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 9285, 9287 ], "id": "AHCKFKLKGB_2", "locus": "GCA013367975_00020" }, { "type": "cds", "sequence": "contig_1", "start": 9566, "stop": 9751, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17676.7" ], "nt": "TTGAAACCTAATGAACTATATGATAAGGTTTTATTTCTTGAAACAAGTGAAGAAAAGCCGAGTCCACAATTGCTTAAAAAAATGTTGCTAAAATTCAAAGAAGTTGGCTTATTTAAAAATATTCAAGGAATATTAGTCGGATGCCCTCAAAATCGTGTTTATTATAACGAATATAAAGAAGTTTGA", "aa": "MKPNELYDKVLFLETSEEKPSPQLLKKMLLKFKEVGLFKNIQGILVGCPQNRVYYNEYKEV", "aa_hexdigest": "df46e7c999a7c8a7683c5cb970627ae8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17676.7", "name": "Peptidase_S66C", "length": 50, "aa_cov": 0.7704918032786885, "hmm_cov": 0.96, "evalue": 4.340136709726667e-10, "score": 35.58340072631836, "start": 4, "stop": 50 } ], "seq_stats": { "molecular_weight": 7222.446000000002, "isoelectric_point": 8.733032417297363 }, "id": "AHCKFKLKGB_61", "locus": "GCA013367975_00021" }, { "type": "cds", "sequence": "contig_1", "start": 10003, "stop": 10491, "strand": "-", "frame": 1, "gene": "mccF", "product": "Microcin C7 resistance MccF related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X0PHG8" ], "nt": "ATGATCAAACCAATAAAATTAAGCAAGGAGAATGGCATTGCAATAGTAAGCTTATCTGCAGGAACTATTGGAGAAGAATTTGCTAGTCACCAATTAGATTTAGCAATAAAAAGACTCAATTTTGATTTTCAACTTAACGTTATGATTGCTAAAAACGCACTTAAGGGAATAAAAGTTTTAAAAGAAAATCCTATGTTAAAAGTTGAGGATTTAAATGAAGTTTTTAGCAATGTCAATGTAAGAGCAATAATTTGTGCAATTGGTGGATTTGACACATACAAAACTATTCCACTTTTATTAGAAGACGAAAACTTTAAAAATTTAGTCTTAAATAATCCTAAAATTTTTATTGGATATTCAGATTCAACCGTTAACCATTTAATGTTTTATAGATTAGGACTAAAAACATTTTATGGGCACAACATTTTATGCGATATTGCTGAACTTGACAAAGAAATGTTGCCTTATACTAAAAACTCATTTAAATGA", "aa": "MIKPIKLSKENGIAIVSLSAGTIGEEFASHQLDLAIKRLNFDFQLNVMIAKNALKGIKVLKENPMLKVEDLNEVFSNVNVRAIICAIGGFDTYKTIPLLLEDENFKNLVLNNPKIFIGYSDSTVNHLMFYRLGLKTFYGHNILCDIAELDKEMLPYTKNSFK", "aa_hexdigest": "04d4575bffa51b51cfdefdaf64bbc0da", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00308136E8", "query_cov": 0.9938271604938271, "subject_cov": 1.00625, "identity": 0.547, "score": 183.0, "evalue": 1.13e-56, "valid": false, "product": "LD-carboxypeptidase", "uniref50_id": "UniRef50_X0PHG8" }, "pscc": { "uniref50_id": "UniRef50_X0PHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X0PHG8" ], "product": "Microcin C7 resistance MccF related protein" }, "genes": [ "mccF" ], "id": "AHCKFKLKGB_62", "locus": "GCA013367975_00022" }, { "type": "cds", "sequence": "contig_1", "start": 10582, "stop": 11304, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E443604", "UniRef:UniRef90_UPI001E443604" ], "nt": "TTGCAATATGCTCAATATATTGAAAAAATAGATTCAACAAATCAACTTTATAAAATATTAGCCAATAAAACATTGCCTGAAACTAATGAAATATTTATTGACCCTGATCAAATAGAAAAAAATAATGTTAAACTAAACAAAACAGAAAATTATGCATATAAAGATTTAAACGATAGACTTCATCATTTTGATTCAAATCCCGAAACATTATTCATTAACGAAGCAAAGAAAATAATTAGTGAATATAAAAATGAAATAAACGTCAAAGTTTATACAAAAAACCCTGTTTTTCATGGTTTAAAATTAGAATATTTTGATGAAGAAAGAGAAATTAAGTCTTCATATCCTGATTTTATTTTTAAAATAAATAATGGTGAAGTAACAAATTATTTATATGTTGAAGTTAAAAGTGCAAAACACGATTATAATCAACAAAAAACTAAAAACATAGTTACTAGCTATAAAAAATATTTAGACAATAAAAAACCTGATAATGCTTTATTTGTTGATGACGTAGAAAAAAATAATAATTACACGTTTTTAATTTGTTATGTTGATGAAGAAAACCAAGACTTTTATTTTTTAGGGGCAAGTGATAATGCAGAACTTGATAAAGAGGTCAATATATCTATTCCTGACGGCATAAAAGAATTAATTATCAATAAACATAAATTTATTTCAGATATTGAATCATTATTCAAATATTTTCAAAACAATAAATAA", "aa": "MQYAQYIEKIDSTNQLYKILANKTLPETNEIFIDPDQIEKNNVKLNKTENYAYKDLNDRLHHFDSNPETLFINEAKKIISEYKNEINVKVYTKNPVFHGLKLEYFDEEREIKSSYPDFIFKINNGEVTNYLYVEVKSAKHDYNQQKTKNIVTSYKKYLDNKKPDNALFVDDVEKNNNYTFLICYVDEENQDFYFLGASDNAELDKEVNISIPDGIKELIINKHKFISDIESLFKYFQNNK", "aa_hexdigest": "0d4601a62da27b1bac8a0f49a50bd3a4", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 10582, "stop": 11604, "observations": { "mutation": [ 11343, 11493 ], "stop": [ 11343, 11493 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E443604", "query_cov": 1.0, "subject_cov": 0.42704626334519574, "identity": 0.983, "score": 475.0, "evalue": 1.07e-164, "gene_start": 1, "gene_end": 240, "reference_start": 323, "reference_end": 562, "reference_sequence": "MIEKKQDLYDLAFFPYLELTPHFSTLPLKNIRPAMLIQVENEPSEKSTAKEKDDFKESIENIIKKLNDCNLSYVKYFSNDKVESSSLIKEENHKINLKEISKNDSNIDAIIFKIGPSTGWNIPRACMLVQLRKVSSSSLSIQTIGRIKRFPNPSYNKDQISREGNRYYIYSNAQTKENIRRTLILKREFENEKFVYGQINKVKIKQAIIGADFIREAKNSIVKEKIDEYFKKYHKEYIKNNKKLIGEQGNIEDKIRVYSWINNSIELELFINKFLLMHKKIFNEVLIEALNEKYDSIYSNNSPITRNIFWYILIKEYFNTFKLQYAQYIEKIDSTNQLYKILANKTLPETNEIFIDPDQIEKNNVKLNKTENYAYKDLNDRLHHFDSNPETLFINEAKKIISEYKNEINVKVYTKNPVFHGLKLEYFDEEREIKSSYPDFVFKINNGEITNYLYIEVKSAKHDYNQQKTKNIVTSYKKYLDNKKPDNALFVDDVEKNNNYTFLICYVDEENQDFYFLGASDNAELDKEVNISIPDGIKELIINKHKFISDIESLFKYFQNNK" }, "paralog": false, "identity": 0.9735294117647059, "score": 672.0, "evalue": 1.98e-244, "description": "Internal stop codon at 11343, 11493. Nonsense mutation around 11343, 11493.", "psc": { "uniref90_id": "UniRef90_UPI001E443604", "uniref50_id": "UniRef50_UPI001E443604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E443604", "UniRef:UniRef50_UPI001E443604" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E443604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E443604" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 28502.56200000004, "isoelectric_point": 5.188058662414549 }, "id": "AHCKFKLKGB_63", "locus": "GCA013367975_00023" }, { "type": "cds", "sequence": "contig_1", "start": 11491, "stop": 11892, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGTGCAACTTAGAAAAGTGTCATCAAGTAGTTTGTCTATTCAAACAATAGGAAGAATTAAAAGATTCCCAAATCCAAGTTATAACAAAGATCAAATTTCTAGAGAAGGCAATAGATACTATATTTATTCAAATGCTCAAACCAAAGAAAATATAAGAAGAACATTGATTTTAAAACGAGAATTTGAAAATGAAAAATTTGTTTATGGCCAAATAAATAAAGTAAAAATCAAACAAGCAATAATAGGAGCAGATTTTATTAGAGAAGCTAAAAATTCTATTGTTAAAGAAAAGATTGATGAATATTTTAAAAAATACCATAAAGAATATATTAAAAACAACAAGAAATTAATAGGAGAACAAGGAAACATTGAAGATAAAATTAGAGTTTATTCATGA", "aa": "MLVQLRKVSSSSLSIQTIGRIKRFPNPSYNKDQISREGNRYYIYSNAQTKENIRRTLILKREFENEKFVYGQINKVKIKQAIIGADFIREAKNSIVKEKIDEYFKKYHKEYIKNNKKLIGEQGNIEDKIRVYS", "aa_hexdigest": "beb3cf193d9d6585f06e5659a021583d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15826.048599999987, "isoelectric_point": 9.932660102844238 }, "id": "AHCKFKLKGB_64", "locus": "GCA013367975_00024" }, { "type": "cds", "sequence": "contig_1", "start": 11911, "stop": 13035, "strand": "-", "frame": 1, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2BAF28" ], "nt": "ATGAATCTAACTAGTGTACAAGAAAAAGCAGTTAAACAATTGCTTGAAGAATATAAAATTGCAATTCAATCTAGTGAACCTAAAATAGTTGATTTTCAAGCTCCAACTGGTAGCGGTAAAACCTTTATGTTGGCTAATACTATAAATGAAATAATAAAAGAAAATAATGCCAATGGTGAACCAAACAAATTAGTCTTTGTTATTGCCACACTTTCATCAGCAGATTTACCTTATCAAATGGAATCAAACCTAAATGAATATAAAAGATATATTAATGGTTTGTATGAAGTAGAAAGAAAGGAATCTCCTTCCTTGAATTCTCAAAAAAATAAAGATTCAACTTATAATTTAATTCCTCAAAAAAATAAAGTAATAATCTTTGGTGTTTCATCATTTGGTAAAAATAGAATATTTACCGAACAAGGTTTGCTTGAAAGCTTTCTTGATCAAATTAAATATCAAAACTATACATTAGTTTATATTAGAGATGAAGCGCATCATGGTGGAAATATAAAAGGTAGAAGTAATTTTAAATTTGAAGATATTGATACAATCCATGATGAATCTAAATTAAAAAAAGAAGAAGTTAGGTTTGAATATAAAATTCAAAAAGCAGCCAATTTAATAATAAAAATGACAGCAACTCCTAAAGGAACACATAAACTTGTCTATGTTGATGAAAAGGATTTAGCTGAAGATGATATTAAATTATTAAAAGATGAATTAGTTTATAACAAGGGTATAACTGACTCGAGTCAAGATTTAATTGATGATAATGAATTACTAAATAAAGCATGTCAAACATTTAAAGAAGTTCAAATCCAATATGCAAACAATAATAGTGATTTAAAAAATATAAGACCAGCAATGCTTATTCAAGTCGAAAATGAACCAAGTGAAAAATCAACAGCCAAAGAAAAAGATGATTTTAAAGAAAGCATTGAGAACATAATTAAAAAATTAAATGAATGTAATTTGAGTTATGTTAAATACTTTTCAAATGATAAAGTGGAATCTTCTTCTTTGATAAAGGAAGAAAATCATAAAATAAACTTAAAAGAAATTTCAAAAAATGACTCTAATGTTGATGCAATAATTTTTAAAATTGGTCCTTCAACTGGTTGA", "aa": "MNLTSVQEKAVKQLLEEYKIAIQSSEPKIVDFQAPTGSGKTFMLANTINEIIKENNANGEPNKLVFVIATLSSADLPYQMESNLNEYKRYINGLYEVERKESPSLNSQKNKDSTYNLIPQKNKVIIFGVSSFGKNRIFTEQGLLESFLDQIKYQNYTLVYIRDEAHHGGNIKGRSNFKFEDIDTIHDESKLKKEEVRFEYKIQKAANLIIKMTATPKGTHKLVYVDEKDLAEDDIKLLKDELVYNKGITDSSQDLIDDNELLNKACQTFKEVQIQYANNNSDLKNIRPAMLIQVENEPSEKSTAKEKDDFKESIENIIKKLNECNLSYVKYFSNDKVESSSLIKEENHKINLKEISKNDSNVDAIIFKIGPSTG", "aa_hexdigest": "3c91c4814b4c8b8788c69e910a030ad1", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0001981E5C", "query_cov": 0.8903743315508021, "subject_cov": 1.021472392638037, "identity": 0.5920000000000001, "score": 363.0, "evalue": 9.65e-122, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_UPI001E2BAF28" }, "pscc": { "uniref50_id": "UniRef50_UPI001E2BAF28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2BAF28" ], "product": "DEAD/DEAH box helicase family protein" }, "genes": [], "id": "AHCKFKLKGB_65", "locus": "GCA013367975_00025" }, { "type": "cds", "sequence": "contig_1", "start": 13022, "stop": 13180, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTGCCAACAACAATGAAATAATTAAACAAGATTTTAAAAACATGTTGAATGATTTTAGCTTATCGAATGTAAATAAAATTAACGAACAAGAAATATTACTAAATTTAACTGCTTTAAAACCAGTTGAAGGAAAAGATAGCAATGAATCTAACTAG", "aa": "MLANNNEIIKQDFKNMLNDFSLSNVNKINEQEILLNLTALKPVEGKDSNESN", "aa_hexdigest": "3fe26d477464b381edc8c24080cc38c5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5906.566599999999, "isoelectric_point": 4.5550428390502935 }, "id": "AHCKFKLKGB_66", "locus": "GCA013367975_00026" }, { "type": "cds", "sequence": "contig_1", "start": 13247, "stop": 13537, "strand": "-", "frame": 3, "gene": null, "product": "Site-specific DNA-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAW9XAF6" ], "nt": "ATGAAAAGTATTTTTTCAGATAAAAATATTTTTGATTTTCCCAAACCAATCGAACTAATTAAGTATTTAATTAACATTCACCCTAATCCCAACGCTCGTGTATTAGATTTCTTTGCAGGTTCAGGAACAACAGGTCATGCAGTTTTAGATTTAAATAAAGAAGATGGTGGCAATAGAACATTCACATTAGTTACAAACAATGAAAATAATATTGGCATAAATGTTTGCTATGAAAGGCTTTATAGAATTAATAATGGCAAGGGAAGTAAGGGTGAAACCGACTTCGATTGA", "aa": "MKSIFSDKNIFDFPKPIELIKYLINIHPNPNARVLDFFAGSGTTGHAVLDLNKEDGGNRTFTLVTNNENNIGINVCYERLYRINNGKGSKGETDFD", "aa_hexdigest": "fd1b8f72ab529cec6e8f773530a50125", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0013684925", "query_cov": 0.90625, "subject_cov": 0.90625, "identity": 0.7240000000000001, "score": 126.0, "evalue": 3.94e-36, "valid": false, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_A0AAW9XAF6" }, "pscc": { "uniref50_id": "UniRef50_A0AAW9XAF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAW9XAF6" ], "product": "Site-specific DNA-methyltransferase" }, "genes": [], "id": "AHCKFKLKGB_67", "locus": "GCA013367975_00027" }, { "type": "cds", "sequence": "contig_1", "start": 13739, "stop": 13879, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTTACTTTTTCGTAATAGTTCAACCGGTAGTTTTCAATATTTGAAATCTCTAGATTATCCAATTGAAGCACCAGATGGAACTACTTTTATGTTGCACGTAAATAAATATAAACCAAAGTCGGGCCGTTATACATGA", "aa": "MTLLFRNSSTGSFQYLKSLDYPIEAPDGTTFMLHVNKYKPKSGRYT", "aa_hexdigest": "3f5a338fe4467b9879b65efd3062cb4b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5305.004600000002, "isoelectric_point": 9.52193202972412 }, "id": "AHCKFKLKGB_68", "locus": "GCA013367975_00028" }, { "type": "cds", "sequence": "contig_1", "start": 14072, "stop": 14221, "strand": "-", "frame": 3, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025CFB7FC" ], "nt": "ATGATGAACGAAAGATTAAAAATGGCTCGCCAACTTTTAAAAGAAGATGGTGTTATTTTTGTTTCAATTGACGACAATGAACAAGCATATTTAAAAGTATTAATGGATGAAATTTTTGGGGAAGAAAATTTTATAACATGTTTTTCTTGA", "aa": "MMNERLKMARQLLKEDGVIFVSIDDNEQAYLKVLMDEIFGEENFITCFS", "aa_hexdigest": "7a9d3ec5dd85a621319ff061dbcc14af", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0025CFB7FC", "query_cov": 0.8979591836734694, "subject_cov": 0.88, "identity": 0.705, "score": 67.0, "evalue": 7.25e-14, "valid": false, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0025CFB7FC" }, "pscc": { "uniref50_id": "UniRef50_UPI0025CFB7FC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025CFB7FC" ], "product": "DNA methyltransferase" }, "genes": [], "id": "AHCKFKLKGB_69", "locus": "GCA013367975_00029" }, { "type": "cds", "sequence": "contig_1", "start": 14361, "stop": 14720, "strand": "-", "frame": 2, "gene": null, "product": "Pseudogen of Type III restriction modification system: methylase (Part 1)", "db_xrefs": [ "RefSeq:WP_175933355.1", "SO:0001217", "UniParc:UPI00158845D2", "UniRef:UniRef100_UPI00158845D2", "UniRef:UniRef50_D1J7F1", "UniRef:UniRef90_D1J7F1" ], "nt": "ATGAAGGATTTAAAAAATTATATAGATGATATAGATAACTTGAATTCTTTTTTATTAAATCAAGACCAAAAGAAATTAATAAAAGAAATTTTAGTTAACACAAACAATTCAAATAAAGAAAACTTGGACAATGTTTTTCAACTTTTGATTCAAAGAGTTAAAACAGGCTTTGTTTTTGATGTTGCACCTAGTGTTGATACAAAACAAATTGCAATATTAAAAAAACAAGAAAAATTATCATTTACAAATGATTTATTGAATACTAATAAAAACACTTTAATTATTGGTGAAAACTATGATGCATTAAAAAACCTACTTGCAATTGAAAGACAAAAAGAACAAATAGGGGGGGGGTTATAA", "aa": "MKDLKNYIDDIDNLNSFLLNQDQKKLIKEILVNTNNSNKENLDNVFQLLIQRVKTGFVFDVAPSVDTKQIAILKKQEKLSFTNDLLNTNKNTLIIGENYDALKNLLAIERQKEQIGGGL", "aa_hexdigest": "adb66a9c16bb9bd2dcce13d4048e8d7f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158845D2", "ncbi_nrp_id": "WP_175933355.1", "uniref100_id": "UniRef100_UPI00158845D2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158845D2", "RefSeq:WP_175933355.1", "UniRef:UniRef100_UPI00158845D2" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158845D2", "uniref90_id": "UniRef90_D1J7F1" }, "psc": { "uniref90_id": "UniRef90_D1J7F1", "product": "Pseudogen of Type III restriction modification system: methylase (Part 1)", "uniref50_id": "UniRef50_D1J7F1" }, "pscc": { "uniref50_id": "UniRef50_D1J7F1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7F1" ], "product": "Pseudogen of Type III restriction modification system: methylase (Part 1)" }, "genes": [], "id": "AHCKFKLKGB_70", "locus": "GCA013367975_00030" }, { "type": "cds", "sequence": "contig_1", "start": 15028, "stop": 15117, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACAAAGAAATAGATGCTAAACAAGCAAAACAAGAAAGAGCAGAAAAATTATTTGGCCAAACACCAATAAAAAAAGCAATTTGA", "aa": "MKNKEIDAKQAKQERAEKLFGQTPIKKAI", "aa_hexdigest": "64c0078cc5b697f9a98ccb8247f59a16", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3342.9089000000004, "isoelectric_point": 9.873542594909669 }, "id": "AHCKFKLKGB_71", "locus": "GCA013367975_00031" }, { "type": "cds", "sequence": "contig_1", "start": 15151, "stop": 15570, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FC234F" ], "nt": "ATGGCTGGATTATATGGATTTATTGACCAAATTTTTATTTTGAATTTGGTTCCAAAAAATAGAAACATCTTTGGAAATAATGAAAGTGAAATTGTTCAATTTTTAAATCCTGCCTTGCATAGTGGTGGATTTTTTAGTGATTATAAGATAATGATTGAACAATATAATTTACATTCAATTACAAAATTAGCTGAAATTACTCCTAATAGTATTGTTTCAACTGCGTCAGCAGTATTTGGCCCATTAATATTATTTTCTAATGCAATAGTATTTTTAGTACCTGTTGGAGCTTCAATTTATTATACTAAATGTGTAAGTAAGCAACTAAAACGGGCTGGCCAAGATTTATGGGCGACGATGTTTTGGTTTACGGTTGCACTTTCTTTATTTGCTACGTTAGTATCTTTTGTGTTTATATGA", "aa": "MAGLYGFIDQIFILNLVPKNRNIFGNNESEIVQFLNPALHSGGFFSDYKIMIEQYNLHSITKLAEITPNSIVSTASAVFGPLILFSNAIVFLVPVGASIYYTKCVSKQLKRAGQDLWATMFWFTVALSLFATLVSFVFI", "aa_hexdigest": "18c33e03fb1f6f68af633214941e2da1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002FC234F", "query_cov": 0.9856115107913669, "subject_cov": 0.8154761904761905, "identity": 0.569, "score": 152.0, "evalue": 1.72e-44, "valid": false, "uniref50_id": "UniRef50_UPI0002FC234F" }, "pscc": { "uniref50_id": "UniRef50_UPI0002FC234F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FC234F" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15496.946199999986, "isoelectric_point": 7.86406307220459 }, "id": "AHCKFKLKGB_72", "locus": "GCA013367975_00032" }, { "type": "cds", "sequence": "contig_1", "start": 15799, "stop": 15909, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTGTGCTATAGTTGCAAATGTAATAAATGTTGCTTGTGATGCGCTATTTATTATTTGCTTTAAAATGGGAGTTTTAGGTGGTGTTATGGCAACCATTGTTGGTTGA", "aa": "MICAIVANVINVACDALFIICFKMGVLGGVMATIVG", "aa_hexdigest": "9a593128d35cc48176198b8083bd7525", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3671.5899, "isoelectric_point": 5.580645561218262 }, "id": "AHCKFKLKGB_73", "locus": "GCA013367975_00033" }, { "type": "cds", "sequence": "contig_1", "start": 16411, "stop": 16563, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACTTAGAATAATTGCCGTTTCATTTGTTGTTGTTTCATTGTTAGCATTTCAAGGTTCAAATGATATTGCAAAGTCTATCTTTGCCTCAATATTTGAAAACTTTATTTGCTTTGTAATAATTGTTCCTATTGGATATGGAATTGCTTGA", "aa": "MSLRIIAVSFVVVSLLAFQGSNDIAKSIFASIFENFICFVIIVPIGYGIA", "aa_hexdigest": "7e3886a0f6f4f97d10d162cf23a13065", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5395.421199999997, "isoelectric_point": 5.820676612854004 }, "id": "AHCKFKLKGB_74", "locus": "GCA013367975_00034" }, { "type": "cds", "sequence": "contig_1", "start": 16917, "stop": 17378, "strand": "-", "frame": 2, "gene": null, "product": "DUF996 domain-containing protein", "db_xrefs": [ "RefSeq:WP_175933357.1", "SO:0001217", "UniParc:UPI00158B61F3", "UniRef:UniRef100_UPI00158B61F3", "UniRef:UniRef50_A0A454C8X1", "UniRef:UniRef90_D1J7F6" ], "nt": "ATGGAAAATAATTCAAAAAACTTGAAAACCAAAGAAAATGCAAAACTTCTAATAATATTTTCAGCAATCTTATTAGGAATAGATGTTATTTATATTATTTGTGGTTCGCTTGTACCAGTATTTGGATTATTATTAGCAATAGCAAGCATCGTCTTTTTTATTCTAACAGTTGTTTATGGATTTAAAACAGGATCTCGCGGTTTAAGTAAAAATGAAATAACAACAAGAAAATTATCAATTTCATTAGTTGCCTTGTTTATAATTTCATTCGTCTTAGCAATTGTTGCTACAGCAATGTTTTTATCAGGTGCTGTTAAAACTGCTATAGCAATATATGGACTTTCAGTAATTGCTAATCTTGCACAATTAGGTGTTTTAATTGCATTATTATGTTTTGCAGTAAAAACTTACAATAGTAAAAACAATTCACACCCAGCCCAACAACAAAATTTAAGACAATAA", "aa": "MENNSKNLKTKENAKLLIIFSAILLGIDVIYIICGSLVPVFGLLLAIASIVFFILTVVYGFKTGSRGLSKNEITTRKLSISLVALFIISFVLAIVATAMFLSGAVKTAIAIYGLSVIANLAQLGVLIALLCFAVKTYNSKNNSHPAQQQNLRQ", "aa_hexdigest": "19a03ebac63f857dbaa2506559215e99", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158B61F3", "ncbi_nrp_id": "WP_175933357.1", "uniref100_id": "UniRef100_UPI00158B61F3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B61F3", "RefSeq:WP_175933357.1", "UniRef:UniRef100_UPI00158B61F3" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B61F3", "uniref90_id": "UniRef90_D1J7F6" }, "psc": { "uniref90_id": "UniRef90_D1J7F6", "uniref50_id": "UniRef50_A0A454C8X1" }, "pscc": { "uniref50_id": "UniRef50_A0A454C8X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1" ], "product": "DUF996 domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_75", "locus": "GCA013367975_00035" }, { "type": "cds", "sequence": "contig_1", "start": 17551, "stop": 18081, "strand": "-", "frame": 1, "gene": null, "product": "DUF996 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1" ], "nt": "GTGAATAATAACTTAAAAACCAAAGAAAATGCAAAACTTCTAATAATATTTTCATCAATCTTATTAGGAATAGATGTTATTTATATTATTTGCGGTTCGCTTGTACCAGTATTTGGATTATTATTAGCAATAGCAAGCATTGTGTTTTTTATTTTAACAGTTGTGTATGGATTTAAAACAGGATCACGTGAACCAAAAAATAGTAATCGAATGATAATAAGAAAATTATCCATTGCATTAATAGCATTGTTTGCAGCAACAATTGCAATAGTTATTGTTGCAATAATAATAGCAATATCTTTATCACTTGGCGCATATAATTATGAAGGCCCAAATGAATATAATCCATTTTTGTTAGTGTCATCAACATCTGCACTATATATTATTGGTTTAATTTTGACCCTTGCAGAATTAGGTGTTTTAATTGCATTATTATGTTTTGCAGTTAAGATCTATTGCGTTAAAAATAACAATAATGTTAATAATGAAACAAGCAACTATGGTGACGACTCTCAAAAGCCTCAAAATTGA", "aa": "MNNNLKTKENAKLLIIFSSILLGIDVIYIICGSLVPVFGLLLAIASIVFFILTVVYGFKTGSREPKNSNRMIIRKLSIALIALFAATIAIVIVAIIIAISLSLGAYNYEGPNEYNPFLLVSSTSALYIIGLILTLAELGVLIALLCFAVKIYCVKNNNNVNNETSNYGDDSQKPQN", "aa_hexdigest": "73f0f9db69a031349cff82e589923eec", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00093E8FF7", "query_cov": 1.0056818181818181, "subject_cov": 0.9619565217391305, "identity": 0.8640000000000001, "score": 262.0, "evalue": 2.78e-87, "valid": false, "uniref50_id": "UniRef50_A0A454C8X1" }, "pscc": { "uniref50_id": "UniRef50_A0A454C8X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1" ], "product": "DUF996 domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_76", "locus": "GCA013367975_00036" }, { "type": "cds", "sequence": "contig_1", "start": 18207, "stop": 19091, "strand": "-", "frame": 2, "gene": "clpB", "product": "Chaperone ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_D1J7F8" ], "nt": "TTGTCTGAAACATTAAAAAAGAGAGTGAAAGGCCAAGATCAAGCAATTGAACTAATCTCTGATGCAATCAAGCGTTCTAAAGCAAACATCAATGACCCAAATAGACCAATTGGTTCGTTCCTATTTTTAGGGCCAACTGGTGTTGGTAAAACTGAACTTGCAAAAAGTTTAGCAGAAGCTTTATTTGATGATGAAAATCACATTGTAAGATTAGACATGTCTGAATATATGGAAAAACACAGCGTGTCAAAATTAATTGGATCGCCTCCAGGTTATATTGGTTATGATAATGGGGGACAACTAACTGAAAAAATTAGACAAAATCCTTATTCAATAGTTTTATTTGATGAAATTGAAAAAGCAAATTCAGATGTTTTAAATATATTGTTACAAATATTGGACAATGGTACATTAACAGATAGTACAGGAAGGGTAGTAAACTTTAAAAACACAATAATTATCATGACTTCAAACATTGGAAGTAGTGAAATTATAAATAAGAGTTTAACCATTGATGGGGTAAGGGCATTATTATTAAGAAGTTTTAGACCAGAATTTATTAATAGAATCGATGAAATAGTACCATTCAACCCATTAAGCCCTGCCGTTGTTTGTGATATTGTAAAACTAGAATTATCAAAATTAAAGAAAAGAGTTATTAATAACCATAATTTAGTAATTAATTTTGACGATAAAATAGTTAAATTCGTTGCTGATAACGCTTACGATTCCGCTTTTGGCGCTCGTCCAATTAAAAGATATATTCAAAAAAACATCGAAAACAAATTAGCAATGGAAATCATAAAAGGAAATTTGGTTGAAGGTGATGTTGCCTTATTAACAGTTTCAAATAATAATGTTATTGAATTACAAACAAATAATTAA", "aa": "MSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN", "aa_hexdigest": "28a3a307c9f5b3ecd2df9033bc508995", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_U4KPN6", "query_cov": 0.9829931972789115, "subject_cov": 0.8376811594202899, "identity": 0.5539999999999999, "score": 315.0, "evalue": 1.22e-103, "valid": false, "product": "ATP-dependent Clp protease ATP-binding subunit, 2", "uniref50_id": "UniRef50_A0A356L436" }, "genes": [ "clpA", "clpB" ], "truncated": "3-prime", "pseudogene": { "start": 18207, "stop": 19391, "observations": { "mutation": [ 19148 ], "stop": [ 19148 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F8", "query_cov": 1.0, "subject_cov": 0.4106145251396648, "identity": 0.997, "score": 563.0, "evalue": 2.76e-196, "gene_start": 1, "gene_end": 294, "reference_start": 423, "reference_end": 716, "reference_sequence": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSKWTNIPVNRLLENQKQKILNLSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN" }, "paralog": false, "identity": 0.9949238578680203, "score": 754.0, "evalue": 1.64e-273, "description": "Internal stop codon at 19148. Nonsense mutation around 19148.", "psc": { "uniref90_id": "UniRef90_D1J7F8", "gene": "ClpA", "product": "Chaperone ClpB", "uniref50_id": "UniRef50_I5D648", "cog_id": "COG0542", "cog_category": "O", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F8", "UniRef:UniRef50_I5D648", "COG:COG0542", "COG:O" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "AHCKFKLKGB_77", "locus": "GCA013367975_00037" }, { "type": "cds", "sequence": "contig_1", "start": 19146, "stop": 20357, "strand": "-", "frame": 2, "gene": "clpB", "product": "Chaperone ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_D1J7F8" ], "nt": "ATGGAAGCAAGTTATACACCTTATGAACAAGAAAAAGATCCCTTGAAGGAATTTGGTCGAAACTTAACTGAGTTAGCTACGCAGAACAAACTAGAACCTGTTATAAACCGTGATGAAGAAATAAGATCACTAGTTAGAATTTTGTCAAGAAAAACTAAAAATAATCCCGTTTTAGTTGGTGAACCCGGTGTAGGTAAAACAGCAATTGTTGAAGGCCTAGCAAGGAAAATCGTTGAAAATCAAGTTCCAGAAAACCTAAAAGGCAAACAATTAATTGAAATTGATTTACCTGCATTGGTTGCAGGAACACAATATCGTGGTCAATTTGAAGAAAGACTAAAAAAAGTATTGAAAAAGATTGACGAAGCAAATGGTGAAATCATTCTTTTCATTGATGAAATTCATATGATAATTGGCGCCGGAGGAACTGGCGATAGCAATATGGATGCAGCAAATATATTGAAACCTATGATGGCAAGAGGTCAATTGCATCTAATTGGGGCAACAACATTGGATGAATATAGAAAATATATTGAAAAAGATCCTGCCCTTGAAAGAAGAATGCAAAAAATTTTAATTTCAGAACCTTCAATTGAAGATACCATAACTATATTGCGTGGTATAAAAGAAAGATTTGAAGCATATCATGAAGTTAAAATTGATGACTCTGCTATAGTTTCGGCAGCAAATTTATCAGCAAGATATATTGCAGATAGATTTTTGCCTGATAAAGCAATAGATTTAATTGATGAAGCAGCATCAAATATAAAAACTGAAATGAACTATTTGCCTGAACCTCTAGAAAAAATTAATTATCAAATTGCTAGATTAGAAATTGAAAAAGCTGCTCTAACAAATGATAGTGAAACAAAGCAAACAGCCATTAAAAATAAAGAAAGAATTTTAGAAATAGATAAAGAACTAAATACTTTAAAATTGCAAAAAGTTGACCTTGAAAAACGCTGGAAGCAAGAAAAAGAAGATATTCAAAAATTTTCTTCAATTAAAGAAAACATTGAAGAATTGAATAGAAAATTGCCTTTACTACAAAGTGAAGGAAAATATGTTGAAGCTTCTAAAATTATGTATGTTTTAGTTCCTGAATTAATCAAAACTAGAGATGAATTGGAACAAAAAATACAATCAAGGAAAAATCGTTTAATCAAGGAAGTTGTTGATGCTGAAGAAGTTGCAGATATTGTTTCAAAATGA", "aa": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSK", "aa_hexdigest": "e7cfa570cca2fe16147fef4315f4855e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI001C99829F", "query_cov": 0.8933002481389578, "subject_cov": 0.975609756097561, "identity": 0.531, "score": 370.0, "evalue": 1.55e-123, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0AA38HIJ9" }, "genes": [ "clpA", "clpB" ], "truncated": "5-prime", "pseudogene": { "start": 18843, "stop": 20357, "observations": { "mutation": [ 19148 ], "stop": [ 19148 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F8", "query_cov": 1.0, "subject_cov": 0.5628491620111732, "identity": 1.0, "score": 753.0, "evalue": 3.45e-269, "gene_start": 1, "gene_end": 403, "reference_start": 1, "reference_end": 403, "reference_sequence": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSKWTNIPVNRLLENQKQKILNLSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN" }, "paralog": false, "identity": 0.996031746031746, "score": 964.0, "evalue": 0.0, "description": "Internal stop codon at 19148. Nonsense mutation around 19148.", "psc": { "uniref90_id": "UniRef90_D1J7F8", "gene": "ClpA", "product": "Chaperone ClpB", "uniref50_id": "UniRef50_I5D648", "cog_id": "COG0542", "cog_category": "O", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F8", "UniRef:UniRef50_I5D648", "COG:COG0542", "COG:O" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "AHCKFKLKGB_78", "locus": "GCA013367975_00038" }, { "type": "cds", "sequence": "contig_1", "start": 21035, "stop": 21268, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAATTTATTGCCATGGGTTTCAGAAATAAATTCATCACTGTTTTTTATTATACTTTCAACCATTAATACAACTGTATTAGGTGCTTTATCAACTTTTTGTCTTTTTATTGCTACAAAAATTAATATTGAAATAATAAAAATTAAAGCAACTAAAATAATTAAAGTAAATAATTGGTTGTCATTGAAACTGCTTACTTCGCCAGGATTAACTGGACCACCAAATCAATGA", "aa": "MSNLLPWVSEINSSLFFIILSTINTTVLGALSTFCLFIATKINIEIIKIKATKIIKVNNWLSLKLLTSPGLTGPPNQ", "aa_hexdigest": "6e03ba3af67b45d900294beb0578c60f", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8459.05179999999, "isoelectric_point": 9.748022651672361 }, "id": "AHCKFKLKGB_79", "locus": "GCA013367975_00039" }, { "type": "cds", "sequence": "contig_1", "start": 21278, "stop": 21685, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTATCAAAAATATCAAAATATAAATTAAATGCTGGCAAGATCAATAACCCAATAGGGTTTAACTCAGCTGAAGGTGATTCTAAGTTAGCGCCAATAATTTTGGTTCAAATATTATTTAAGAATAAACTTACTAGTGCAACCAATACAACTCCTCCAAGTATGTTTCCATACATACGGAAAGTTAAACTTATTAATGGTGAAAAGTTTGATGATGCTTCTAAGGGATTAAGAATTTTAAATAAATACTTAAATTTTTTATATATGAATCCAATGATGTGTGTTCCAAGTCAACTTATAACAGTGGCAGCAAAAATTGCTGACAAACTAGTTCCAATTGGGGCTAAACCAACTAATGAAAGCATATTTCCTAAAATCAAGAAGGCAAATAATGAAATAAAATAA", "aa": "MKLSKISKYKLNAGKINNPIGFNSAEGDSKLAPIILVQILFKNKLTSATNTTPPSMFPYIRKVKLINGEKFDDASKGLRILNKYLNFLYMNPMMCVPSQLITVAAKIADKLVPIGAKPTNESIFPKIKKANNEIK", "aa_hexdigest": "cd316ae9f96adac2d24ff4871f142a36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14983.714699999984, "isoelectric_point": 10.005122566223147 }, "id": "AHCKFKLKGB_80", "locus": "GCA013367975_00040" }, { "type": "cds", "sequence": "contig_1", "start": 21883, "stop": 22188, "strand": "+", "frame": 1, "gene": "atpE", "product": "ATP synthase F0 subunit C", "db_xrefs": [ "COG:C", "COG:COG0636", "EC:7.1.2.2", "GO:0015078", "GO:0015986", "GO:0045263", "RefSeq:WP_175933360.1", "SO:0001217", "UniParc:UPI00158D3D58", "UniRef:UniRef100_UPI00158D3D58", "UniRef:UniRef50_B3PLV3", "UniRef:UniRef90_D1J7G1" ], "nt": "ATGGATAAATTAGAAAATATTGTTAATGCATTCAATACAGCATCAGCAGGTGTTAATAGTAAAGAAGCAGGAGTGAACTACCCTCTAGCATATGGTTTAACAATGTTAGGAGCAGGTATTGCTGTTCTTGGCTCAGGTTTAGTTTCTATTGGTCAAGGTATAGCCGTTGCTAAAGCCTGTGAAGCTGTTGGAAGAAATCCAGAAGCATTTTCAAAAATTCGTACATTGTTAATTTTGGGTTTAGGTATTGTTGAAACCGGATCTATTTATTGTTTAGTTATTTCATTATTGCTAATCTTCGTTTAA", "aa": "MDKLENIVNAFNTASAGVNSKEAGVNYPLAYGLTMLGAGIAVLGSGLVSIGQGIAVAKACEAVGRNPEAFSKIRTLLILGLGIVETGSIYCLVISLLLIFV", "aa_hexdigest": "c879cecb8f47ebfc100ac9fe218b5340", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158D3D58", "ncbi_nrp_id": "WP_175933360.1", "uniref100_id": "UniRef100_UPI00158D3D58", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D3D58", "RefSeq:WP_175933360.1", "UniRef:UniRef100_UPI00158D3D58" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D3D58", "uniref90_id": "UniRef90_D1J7G1" }, "psc": { "uniref90_id": "UniRef90_D1J7G1", "gene": "atpE", "product": "ATP synthase F0 subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_B3PLV3", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0015078", "GO:0015986", "GO:0045263" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLV3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLV3" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "AHCKFKLKGB_81", "locus": "GCA013367975_00041" }, { "type": "cds", "sequence": "contig_1", "start": 22193, "stop": 22318, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCAAAATTTTGCAAATTTATTAAGCCAATCGCAAAATGCAATTGCAGCAGCTAAGCCATCTGCTTCTGCTAAAATTGCAGAAGAACTAAACAAAACTTTTAATGGTTTAACATTTAATTGA", "aa": "MFQNFANLLSQSQNAIAAAKPSASAKIAEELNKTFNGLTFN", "aa_hexdigest": "14512e228ffe99de195f5f6ed3e87fe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4401.906899999999, "isoelectric_point": 8.254934883117677 }, "id": "AHCKFKLKGB_82", "locus": "GCA013367975_00042" }, { "type": "cds", "sequence": "contig_1", "start": 22361, "stop": 22774, "strand": "+", "frame": 2, "gene": "atpF", "product": "ATP synthase subunit b", "db_xrefs": [ "COG:C", "COG:COG0711", "SO:0001217", "UniRef:UniRef50_B3PLV4", "UniRef:UniRef90_D1J7G2" ], "nt": "TTGATTATTACTTTATTGGTTTATAAACCCATAAAGAAATTAATTAAAAACCGCCAAGAACTTATTCAAAAAAATATTGATACTTCAATATTAGCAAAAGAAAATGCTTTAAAAGTTCAAGAAGAAAATGATAAAAAAATTATTGAAGCTAATAATCAAGCAGCTTTAATAATTAATCATGCTAAATCAGAAAGTGAAAGAATTGTCAACGCAAGCACTATTGCTGCTAAAAAACAATCTGAACTTTTATTAGATCAAGCACAAATTTTGATTAATAAAAAGCAATCTGAGCTAGAACAAGCGCAAAAGAAAATAATAGTAGAAAATGCTGTTGAATTAGCCAAGAAAATTATTGGCCGTGAAATTAGAAATGAAGATAACATTAAATTAATTAATGAAGTCTTAGAAGATTAA", "aa": "MIITLLVYKPIKKLIKNRQELIQKNIDTSILAKENALKVQEENDKKIIEANNQAALIINHAKSESERIVNASTIAAKKQSELLLDQAQILINKKQSELEQAQKKIIVENAVELAKKIIGREIRNEDNIKLINEVLED", "aa_hexdigest": "be3df468688ee76264db8a0e355bc9c6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "atpF" ], "truncated": "5-prime", "pseudogene": { "start": 22193, "stop": 22774, "observations": { "mutation": [ 22316 ], "stop": [ 22316 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7G2", "query_cov": 1.0, "subject_cov": 0.7098445595854922, "identity": 0.993, "score": 228.0, "evalue": 2.8e-74, "gene_start": 1, "gene_end": 137, "reference_start": 57, "reference_end": 193, "reference_sequence": "MFQNFANLLSQSQNAIAAAKPSASAKIAEELNKTFNGLTFNWPFFVFSLISLGLVVLIITLLVYKPIKKLIKNRQELIQKNIDTSILAKENALKVQEENDKKIIEANNQAALIINHAKSESERIVNASTIAAKKQSELLLDQAQILINKKQSELEQAQKKIIVENAVELAKKIIGREIRNEDNIKLINEVLED" }, "paralog": false, "identity": 0.9948186528497409, "score": 344.0, "evalue": 1.75e-122, "description": "Internal stop codon at 22316. Nonsense mutation around 22316.", "psc": { "uniref90_id": "UniRef90_D1J7G2", "gene": "AtpF", "product": "ATP synthase subunit b", "uniref50_id": "UniRef50_B3PLV4", "cog_id": "COG0711", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7G2", "UniRef:UniRef50_B3PLV4", "COG:COG0711", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLV4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLV4" ], "product": "ATP synthase subunit b" } }, "id": "AHCKFKLKGB_83", "locus": "GCA013367975_00043" }, { "type": "cds", "sequence": "contig_1", "start": 22848, "stop": 23315, "strand": "+", "frame": 3, "gene": "atpH", "product": "F0F1 ATP synthase subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "SO:0001217", "UniRef:UniRef50_D1J7G3", "UniRef:UniRef90_D1J7G3" ], "nt": "TTGGCATTAGTTTCTAATGAAGCTGCTGAAATTACAAGACTTTTAAAGAAAAATAAAGATTATTTGTATGTTTTAAATTCTTATGATATTGACGAAAATGAAAAATATCGTCTAATTGATGAAGCTTTTTCAAGATACAATTTTTATTTAGTAAATACTCTTAAATTAGCAGCTAAGCAACATGTGATTAAATACATTATTGTAATATTAACAAAATTTGTTGAACTTTCAAATGAAAAAAGAAACATTAAATACGGGTTTGTTTATACAATATCGCCTTTATCTGAAAGAGAATTAAAAAGGTTAGAAGTAAAACTTTCAAACGAGTTAAAGGCTAATATTCATTTAACAAATGAAATTGATCCCAAATTAATAGCGGGAATCAAAATAAAAGTTGACGATTATTTAATCGATAATTCAATAGTCAACAAATTAAATGACTTTAAAAAAGTCGTAGAAAATAATTAA", "aa": "MALVSNEAAEITRLLKKNKDYLYVLNSYDIDENEKYRLIDEAFSRYNFYLVNTLKLAAKQHVIKYIIVILTKFVELSNEKRNIKYGFVYTISPLSERELKRLEVKLSNELKANIHLTNEIDPKLIAGIKIKVDDYLIDNSIVNKLNDFKKVVENN", "aa_hexdigest": "c2aedb1828d349ce96d24aa5aed5c9c3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7G3", "query_cov": 1.0, "subject_cov": 0.8659217877094972, "identity": 0.987, "score": 293.0, "evalue": 4.58e-100, "valid": true, "gene": "AtpH", "product": "F0F1 ATP synthase subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_D1J7G3", "cog_id": "COG0712", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_D1J7G3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7G3" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "AHCKFKLKGB_84", "locus": "GCA013367975_00044" }, { "type": "cds", "sequence": "contig_1", "start": 23328, "stop": 24074, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGTCATTAGAACCAAATGATTTGTCAGCAATCATTAAGGCGCAAATAAAACACTTTAATGAAGACATATCTAAACAAGAAATTGGCCGTGTAATAACAGTTGGTGATGGTATTGCCTTAGTAAGTGGCTTGAGCAATGTAGAATATGGCGAATTGGTAAAATTCGAATCCGGCGTTATTGGAATGGCCTTGAATCTTGAAGAAGATTTAGTTGGTATAGTTGTCATGGGTGATGACCGCAACATTGTTGAAAATAGTACAGTTTATCGTACTAAACAAGTTATATCAACACCTGTTGGTGACCATTTATCAGGCCGTGTGTTAGATGCACTTGCTAACCCAATTGATGGAAAAGGTCCAATTCAATATGAATATACTAGACCAATTTTTAAAATTGCACCTGGTGTTATGACAAGAAAAGAAGTTAATCAACCACTTGAAACTGGAATTTTGGCAATTGATGCTATGATTCCTATTGGTAAAGGTCAACGTGAATTGATTATTGGCGATAGACAAACAGGTAAGACCGCTATAGCCATTGATGCAATTTTAAATCAAAAAGGTCGCAATGTTTATTGTATTTATGTTGCTGTAGGTCAAAAGAATTCAACTATTGCACAAATAGTTGATCAATTAGCAAAACATGATGCATTAAAATATACAACAATTATTTCTGCATCAGCATCAGACAGTGCTCCTTTACAATATATTGCACCATATACAGGAATTACTATTGCCGAAGAATGA", "aa": "MSLEPNDLSAIIKAQIKHFNEDISKQEIGRVITVGDGIALVSGLSNVEYGELVKFESGVIGMALNLEEDLVGIVVMGDDRNIVENSTVYRTKQVISTPVGDHLSGRVLDALANPIDGKGPIQYEYTRPIFKIAPGVMTRKEVNQPLETGILAIDAMIPIGKGQRELIIGDRQTGKTAIAIDAILNQKGRNVYCIYVAVGQKNSTIAQIVDQLAKHDALKYTTIISASASDSAPLQYIAPYTGITIAEE", "aa_hexdigest": "09d81e32fbab4285a474e274502644c0", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.9717741935483871, "subject_cov": 0.9450980392156862, "identity": 0.602, "score": 294.0, "evalue": 1.19e-97, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "AHCKFKLKGB_85", "locus": "GCA013367975_00045" }, { "type": "cds", "sequence": "contig_1", "start": 24075, "stop": 24776, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9F0" ], "nt": "ATGGCAAGAGGTAAGGATGTTTTAATTATATATGACGATTTGTCAAAGCATGCTGTTGCATATCGTACATTATCATTACTATTAAGAAGACCACCTGGACGTGAAGCTTATCCTGGAGACGTCTTTTATATTCACTCACAACTTCTAGAACGTGCAGCTAGAGTAAATTCTGATTATGGAGGCGGTTCAATTACTGCTTTGCCAATTATTGAAACTCAAGCTGAAGATATTGCAGCATATATTCCAACAAATGTTATTTCAATAACTGATGGTCAAATCTTTACTAAGGAAGCATTGTTTAACTCTGGCCAACGTCCTGCTATTGATATCGGTTATTCAGTTAGCCGTGTTGGATCAGCAGCACAAACAAAATTAACAAAAAAGGTTGTATCAAGTCTAAAATTGGAATTAGCACAATACAATGAAATGTTAGCATTTGCTCAATTTGGTAGTGATCTAGACCAAGCAACTAGAACTGTTTTGGATCACGGTGCTAAAGTGTATGAATTATTGAAACAACCTCAATATTCTCCATATTCTTCAATAGAACAAATAATTATTCTATTTTGCACAAAATATCGTTTAATAAATGTAATTCCAAAAGAACAAATATTAAAATATAAAACTGATATTTTAAATTACTTTAGAACTAATCCTAATGCTATTAATACATTAAGAGCCGTTTCTGAAGCAAATGATTGA", "aa": "MARGKDVLIIYDDLSKHAVAYRTLSLLLRRPPGREAYPGDVFYIHSQLLERAARVNSDYGGGSITALPIIETQAEDIAAYIPTNVISITDGQIFTKEALFNSGQRPAIDIGYSVSRVGSAAQTKLTKKVVSSLKLELAQYNEMLAFAQFGSDLDQATRTVLDHGAKVYELLKQPQYSPYSSIEQIIILFCTKYRLINVIPKEQILKYKTDILNYFRTNPNAINTLRAVSEAND", "aa_hexdigest": "b468ffcffd554434878d00167a9a7f5b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M9F0", "query_cov": 0.9141630901287554, "subject_cov": 0.841897233201581, "identity": 0.653, "score": 274.0, "evalue": 7.09e-90, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9F0" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9F0" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "AHCKFKLKGB_86", "locus": "GCA013367975_00046" }, { "type": "cds", "sequence": "contig_1", "start": 24890, "stop": 25108, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00231.24" ], "nt": "ATGGAAAGTTTGCAAAAAATAAAACATAGAATTAATTCGGTTAATTCAACTAAAAAAATTACCAAAGCAATGGAGCTAGTTGCAACTGCTAAGTTTGCTAAAATTAAAAATCAATTTGAAAATATCAATAATTATTATCAAAGTGTTGAAAGAGTTTTTTTAAACTTAATTAAAAATTCTGATCAAAATATTGATAAATTATTGAACAGTGCTTCTTGA", "aa": "MESLQKIKHRINSVNSTKKITKAMELVATAKFAKIKNQFENINNYYQSVERVFLNLIKNSDQNIDKLLNSAS", "aa_hexdigest": "04b8f1f836b6c54b2edfbab0ccaf8896", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00231.24", "name": "ATP-synt", "length": 64, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 1.625292054539671e-18, "score": 62.676998138427734, "start": 3, "stop": 66 } ], "seq_stats": { "molecular_weight": 8315.497499999998, "isoelectric_point": 9.85201015472412 }, "id": "AHCKFKLKGB_87", "locus": "GCA013367975_00047" }, { "type": "cds", "sequence": "contig_1", "start": 25184, "stop": 25771, "strand": "+", "frame": 2, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "KEGG:K02115", "SO:0001217", "UniRef:UniRef50_D1J7G5", "UniRef:UniRef90_D1J7G5" ], "nt": "ATGTATAAAAAAATTACTCAAACTGTTGGAAAAGAAGATATCCTAATAGTAGTTGGTAGCAAATTATTGAGTTTAATTCAAAAAGATAATAGCCATCATATTATTCAAATATTAACTCAAATTGGTGACAATCCAAGTTACGAAATCGCTCAAATATTGTCAAATAAGATTTATCATGTTTTAGAAATATCATGGCTAAAATCTATTAAACTTATTTATACTCAATACATTAATCCAATAAAATCTGAACCAATAGTAAAAGAAATTTTTCCAATTTCTATTGATACACTATCAAATAATGATAACAATTTAGCAGGGGAAGAAAGTTCAAAAAGTATTGATCCTTCTTGCTTTGAACCAAACCCTACTGTAATATTGCAAAATTCATTTTCAATATTCTTTGAAGCCTCTATATACAATGCCTTAGCAAATGCTAAGCTTGCAGAAATGAGTCAAAGAAGAACAGCAATGGAACAAGCAAGTGATAATGCACAAGATCTAATTAATGAATTAAATGTTCAATACAATAGTTCTCGTCAAGCAAAAATTACACAAGAAATTACTGAAATAATTAATGGATCAAATTAA", "aa": "MYKKITQTVGKEDILIVVGSKLLSLIQKDNSHHIIQILTQIGDNPSYEIAQILSNKIYHVLEISWLKSIKLIYTQYINPIKSEPIVKEIFPISIDTLSNNDNNLAGEESSKSIDPSCFEPNPTVILQNSFSIFFEASIYNALANAKLAEMSQRRTAMEQASDNAQDLINELNVQYNSSRQAKITQEITEIINGSN", "aa_hexdigest": "6ed4b614693c566f03fdcc8ec32644f5", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "5-prime", "pseudogene": { "start": 24890, "stop": 25771, "observations": { "mutation": [ 25106 ], "stop": [ 25106 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7G5", "query_cov": 1.005128205128205, "subject_cov": 0.6666666666666666, "identity": 0.995, "score": 362.0, "evalue": 6.82e-125, "gene_start": 1, "gene_end": 195, "reference_start": 99, "reference_end": 294, "reference_sequence": "MESLQKIKHRINSVNSTKKITKAMELVATAKFAKIKNQFENINNYYQSVERVFLNLIKNSDQNIDKLLNSASWKFKSKRNLYIVFGSDLGLCGPYNSEMYKKITQTVGKEDILIVVGSKLLSLIQKDNSHHIIQILTQIGDNPSYEIAQILSNKIYHVLEISWLKSIKLIYTQYINPIKSEPIVKEIFPISIDTLSTNNDNNLAGEESSKSIDPSCFEPNPTVILQNSFSIFFEASIYNALANAKLAEMSQRRTAMEQASDNAQDLINELNVQYNSSRQAKITQEITEIINGSN" }, "paralog": false, "identity": 0.9931972789115646, "score": 561.0, "evalue": 1.31e-205, "description": "Internal stop codon at 25106. Nonsense mutation around 25106.", "psc": { "uniref90_id": "UniRef90_D1J7G5", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_D1J7G5", "cog_id": "COG0224", "cog_category": "C", "kegg_orthology_id": "K02115", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7G5", "EC:7.1.2.2", "UniRef:UniRef50_D1J7G5", "COG:COG0224", "COG:C", "KEGG:K02115", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7G5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7G5" ], "product": "ATP synthase gamma chain" } }, "id": "AHCKFKLKGB_88", "locus": "GCA013367975_00048" }, { "type": "cds", "sequence": "contig_1", "start": 25788, "stop": 27275, "strand": "+", "frame": 3, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_020002442.1", "SO:0001217", "UniParc:UPI00036A1184", "UniRef:UniRef100_A0A2K9YSL9", "UniRef:UniRef50_Q5FKY0", "UniRef:UniRef90_D1J7G6" ], "nt": "ATGGCAAATAAAAAAACTAATGAAAATCAAGAAATTCAAGAAGAACAAAACCCAGTTGATGAATCTAAAAATTCAGAAAATTATGGCGTTATTAGCCAAATTTTGGGATCTGTTATTGACGTTAGATTTGAAGAAGGACATACTCCAAAAATCTTAAATGCTTTAAAAATTATTGATGAAGACAAAAAATTTAGCAATGAACAAATTCTTGAAGTTGCTCAACACGTAGGAAATGATACAGTTAGAACAATAGCAATGAACTTGACTATTGGTTTAAAGCGTGGTATGAAGGTTCTTGATTTGGGTTCTCCAATAAAGGCGCCTGTTGGTAAAGAAGTTCTATCTAGAATGTTTAATGTTCTTGGTGAACCTATAGACCAAAAAGGTGGTGTATTCAAACACAAAATGCCTATCCATAGAGCAGCGCCAAGCTACGAAGAACAAAAATCAACACTTGAAATATTTGAAACAGGAATTAAAGTTATTGACTTACTTGTTCCATATATTAAAGGTGGAAAGATAGGTTTATTCGGAGGAGCCGGAGTTGGTAAAACTGTTTTAATTCAAGAATTAATTAACAATATTGCAAAACAACACGGCGGTCTTTCTGTGTTTGCAGGTGTTGGAGAACGTACAAGGGAAGGTAATGACCTTTACCACGAAATGCAAGCAAGTGGTGTTCTAGAAAAAACTGCTCTAGTATTCGGTCAAATGAACGAACCTCCTGGAGCCAGAATGAGAGCACCATTTACTGCTTTAACTATCGCTGAATACTTTAGAGATGTTATGCATCAAGATGTGCTATTGTTTATTGATAACATATTTAGATTTACACAAGCAGGTTCTGAAGTTTCTGCATTGTTAGGTCGTATGCCTTCTGCTGTTGGATATCAACCAACATTGGCTGTTGAAATGGGTCAATTACAAGAAAGAATTACCTCAACCAATAACGGATCAATTACCTCTGTTCAAGCTGTTTATGTTCCTGCCGATGACTTAACAGACCCCGCACCAGCAACAACATTTACACACCTTGATGCAAAAACTGTTTTGGACCGTGATATTGCTGCTTTAGGTATTTACCCTGCTATCGATCCTTTGGCTTCATCATCAAGAATGGTAGATCCAAATATAATTGGTCTTGAACACTATAACACTGCTAGAGCAGTTCAAAATATTCTTCAAAGATTTAAGGAATTACAAGATATTATTGCTATATTAGGTATGCATGAATTAAGTGAAGAAGATAAGAAAGTTGTTGCAAGAGCAAGAAGAATAAGAAACTTCTTATCTCAACCTTTCTTTGTTGCCGAAAAATTCTCTGGTATTAAAGGACAATACATAAAATTGTCAGATACTATTAGATCATTTAAAGAAATTTTAAGTGGAAAATATGATGAATTGCCAGAAGAAGCATTCCTTTATGTGGGTACCATTGACGATGTTATAGAAAAAGCTAAAAGACTTGGGGTTAATGTTGAAAAATAA", "aa": "MANKKTNENQEIQEEQNPVDESKNSENYGVISQILGSVIDVRFEEGHTPKILNALKIIDEDKKFSNEQILEVAQHVGNDTVRTIAMNLTIGLKRGMKVLDLGSPIKAPVGKEVLSRMFNVLGEPIDQKGGVFKHKMPIHRAAPSYEEQKSTLEIFETGIKVIDLLVPYIKGGKIGLFGGAGVGKTVLIQELINNIAKQHGGLSVFAGVGERTREGNDLYHEMQASGVLEKTALVFGQMNEPPGARMRAPFTALTIAEYFRDVMHQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAVEMGQLQERITSTNNGSITSVQAVYVPADDLTDPAPATTFTHLDAKTVLDRDIAALGIYPAIDPLASSSRMVDPNIIGLEHYNTARAVQNILQRFKELQDIIAILGMHELSEEDKKVVARARRIRNFLSQPFFVAEKFSGIKGQYIKLSDTIRSFKEILSGKYDELPEEAFLYVGTIDDVIEKAKRLGVNVEK", "aa_hexdigest": "4a19b542e91cd0c2c735d0c3c4fad8a1", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00036A1184", "ncbi_nrp_id": "WP_020002442.1", "uniref100_id": "UniRef100_A0A2K9YSL9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036A1184", "RefSeq:WP_020002442.1", "UniRef:UniRef100_A0A2K9YSL9" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YSL9", "uniref90_id": "UniRef90_D1J7G6" }, "psc": { "uniref90_id": "UniRef90_D1J7G6", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q5FKY0", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q5FKY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5FKY0" ], "product": "ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "AHCKFKLKGB_89", "locus": "GCA013367975_00049" }, { "type": "cds", "sequence": "contig_1", "start": 27275, "stop": 27652, "strand": "+", "frame": 2, "gene": "atpC", "product": "ATP synthase epsilon chain", "db_xrefs": [ "COG:C", "COG:COG0355", "RefSeq:WP_012855306.1", "SO:0001217", "UniParc:UPI0001BC1FD9", "UniRef:UniRef100_D1J7G7", "UniRef:UniRef50_A0A0C6FPC1", "UniRef:UniRef90_D1J7G7" ], "nt": "ATGGAAACTCCTAAGATACATATTATTATTAGTACTCCATTAGGAATATACTTTGATAAAGAAGCAAGTATTTGTACATTTACTACTACTGAAGGGCAAATTGGAATTATGGCAGGCGCAACTGAGTTTATGGCAGCCTTGGTTCCTTCAAAAATAATGGTTAATTTTTTAAATTCACCTGATTCTAAAGTTTTTTATATTGATAAGGGAATTGTTCAATTTAAAAATAATGTCTTATCAATAATAGTTAATCAAATTGATACTAAAGAAATTGAATTGGATCATAAATTTAGCCCCGAAGATCAAAAGAAATATACAATTATTGAAGAAGTTACTTTGAAAAAGAGCATTTTGAAAAGTCAAACAAACAATAAATAA", "aa": "METPKIHIIISTPLGIYFDKEASICTFTTTEGQIGIMAGATEFMAALVPSKIMVNFLNSPDSKVFYIDKGIVQFKNNVLSIIVNQIDTKEIELDHKFSPEDQKKYTIIEEVTLKKSILKSQTNNK", "aa_hexdigest": "0a62504168c594b2b20b89e019f93a7b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC1FD9", "ncbi_nrp_id": "WP_012855306.1", "uniref100_id": "UniRef100_D1J7G7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC1FD9", "RefSeq:WP_012855306.1", "UniRef:UniRef100_D1J7G7" ] }, "ips": { "uniref100_id": "UniRef100_D1J7G7", "uniref90_id": "UniRef90_D1J7G7" }, "psc": { "uniref90_id": "UniRef90_D1J7G7", "gene": "AtpC", "product": "ATP synthase epsilon chain", "uniref50_id": "UniRef50_A0A0C6FPC1", "cog_id": "COG0355", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_A0A0C6FPC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0C6FPC1" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "AHCKFKLKGB_90", "locus": "GCA013367975_00050" }, { "type": "cds", "sequence": "contig_1", "start": 27909, "stop": 28541, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0", "UniRef:UniRef90_UPI00215D6119" ], "nt": "ATGAAAAAAACAGCAAAAGCTTTATTAATAATATGCACAACTATAATACCTAGTTTTGCTATGCCATTAATATCGGCGGGATGTAATAAAACAACAAACCATGAAACAAACAAAGAAAACACAATTATAGATAAACAGGCCCCTATTAAAAAACAAGAAAATGATTCTAATAATCCCAATAAGTTAGAATCTAATAGTGAATTAGCAGAAGCAGTTAAAAAAGTTAATTTTCAATATAAAGATATCGATAAAATTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAATAACCTTGATAAAAAATTTAAAATAGAAATTTTAAAATTAGAAGCAGACAAGAAGAATAATTTAATATCGATCTCGTATAATATTTCGTTTAATGACGGAAATAAAATCACTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAATAATGAAAGCTATTTAAATGGATTATTGAATGATAATGACACAATGTTTAATTTAACAACTAATCTCAAAAAAGGTAATGAAATAGATGCATATCATTTAGCTAATGGCCAAGGTTCTATTAGTTTGTTGGCATCTAATAAAAATGTTTATATTCCCAAAGATCTAATCTTAGAATATACTTGA", "aa": "MKKTAKALLIICTTIIPSFAMPLISAGCNKTTNHETNKENTIIDKQAPIKKQENDSNNPNKLESNSELAEAVKKVNFQYKDIDKISMFDKLNKENLSNNLDKKFKIEILKLEADKKNNLISISYNISFNDGNKITKLENQKYSLTGLSTNNESYLNGLLNDNDTMFNLTTNLKKGNEIDAYHLANGQGSISLLASNKNVYIPKDLILEYT", "aa_hexdigest": "6e0eeb3f3fd7e2a3d4a2a14b264aa286", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 27909, "stop": 28844, "observations": { "mutation": [ 28539 ], "stop": [ 28539 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D6119", "query_cov": 1.0, "subject_cov": 0.40540540540540543, "identity": 0.971, "score": 384.0, "evalue": 4.49e-130, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MKKTAKALLIICTTITPSFAMPLISAGCNKTTNHETNKENTIIDKQAPIKKQENDSNNPNKLESNSELAEAVKKANFQYKDIDKISMFDKLNKENLSNNLDKNFKIEILKLEADKKNNLISISYNISFNDGNKITKLENQKYSMTGLSTNNESYLNGLLNDNDTMFNLTTNLKKGSEIDAYHLANGQGSISLLASNKNVYVPKDLILEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSIDSFNEGDIEQTLRNNFIPKFENSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVAGFKGIEKVDDEQAKKIVDKLTFGDVYFPEKVKTFSQSRLNKLNLYIKDENGKAQLENPLNFVEYFTKNGINNFNKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKN" }, "paralog": false, "identity": 0.9614147909967846, "score": 593.0, "evalue": 1.85e-214, "description": "Internal stop codon at 28539. Nonsense mutation around 28539.", "psc": { "uniref90_id": "UniRef90_UPI00215D6119", "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D6119", "UniRef:UniRef50_UPI00215CD1C0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23626.61150000002, "isoelectric_point": 8.629689598083495 }, "id": "AHCKFKLKGB_91", "locus": "GCA013367975_00051" }, { "type": "cds", "sequence": "contig_1", "start": 29244, "stop": 29972, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0", "UniRef:UniRef90_UPI00215CD1C0" ], "nt": "TTGAATGAAAAAGAATACACAATTGAAACAAAATTAACGCATGAATCAAAACCAGATGAATGGGTCAACGAAGTTGGTAAGGAATTTTTAGGAAACCCAAATGAAATTTTGGCAAGTGATTTCTCGGCTACAAATTTAAAATTAAATAAGAATGTTGGCGATAGTCTTGACAGTTTAAATTTTATTAAATTATCTTCAATAAAAAGACCTTCAAAAAATTCAGAGGAATTAAAACTCAACGAAGAAGGCAAGGCAACGTTAATTTATGAATTTAGAAATATATATGATAATTCAATATATACAAAAGAAGTCGTATTTGAAAATCTAAAAAAAGTAGAAAAAGCTGATTATAAAAAACAACACGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTAAAAGTGGTTGGGCTACTGACGAAGAAATAAAGAAATTAGAAACTTTTTTGCCAGGCGAAAAAAAATCTAAAAAAAGTAAGAGAATAAACATTGAACATTATATAAATAATGGCAATATAGAAATGCTTGTTGGAAGAGTAATTGTTAAAAAAAATAAAATTACTTTCGGCGATGCATTTAAAAGACAAAAAGAATTTAAAGAAATAGTATTTGATGAAGAATTTGTTGAAGCAGTTAAGAAGTCGTATGATAATAAAAGCGAAAATAAAATATTATCTAATGTAATAACAGATGAAAATTCTAAACGTAAAAATCAAAAGAATTGA", "aa": "MNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKNSEELKLNEEGKATLIYEFRNIYDNSIYTKEVVFENLKKVEKADYKKQHASLIQKINDKKIIKSGWATDEEIKKLETFLPGEKKSKKSKRINIEHYINNGNIEMLVGRVIVKKNKITFGDAFKRQKEFKEIVFDEEFVEAVKKSYDNKSENKILSNVITDENSKRKNQKN", "aa_hexdigest": "fc58186ee5ad11228e8f5013e76cfa59", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 28944, "stop": 30068, "observations": { "mutation": [ 29970, 30000 ], "stop": [ 29970, 30000 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215CD1C0", "query_cov": 1.0, "subject_cov": 0.44814814814814813, "identity": 0.9420000000000001, "score": 426.0, "evalue": 2.04e-145, "gene_start": 1, "gene_end": 242, "reference_start": 267, "reference_end": 508, "reference_sequence": "AYHLANGQGSISLLASNKNVYVPKDLILEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSIDSFNEGDIEQTLRNNFIPKFENSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVAGFKGIEKVDDEQAKKIVDKLTFGDVYFPEKVKTFSQSRLNKLNLYIKDENGKAQLENPLDFAEYFTKNGINNFNKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKNSEELKLNEEGKATLIYEFRNIYDNSIYIKEVVFENLKKVEKADYKKQHASLIQKINDKKIIKSGWATAKEIKKLETFLPSQKKSKKNKGINIEHYINNGNTEILVGRVITKKNKITFGDAFKRQKEFKEIVFDEEFVEAVKKSSNNKSENKILSNVITDENSKHKNQKNWIRLTKHEGKWCILFTFIEDTKSTPYYVQIEK" }, "paralog": false, "identity": 0.9438502673796791, "score": 699.0, "evalue": 2.21e-255, "description": "Internal stop codon at 29970, 30000. Nonsense mutation around 29970, 30000.", "psc": { "uniref90_id": "UniRef90_UPI00215CD1C0", "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215CD1C0", "UniRef:UniRef50_UPI00215CD1C0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 28145.652200000048, "isoelectric_point": 9.065947151184083 }, "id": "AHCKFKLKGB_92", "locus": "GCA013367975_00052" }, { "type": "cds", "sequence": "contig_1", "start": 30309, "stop": 31001, "strand": "+", "frame": 3, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5ADF", "UniRef:UniRef90_UPI00215D5ADF" ], "nt": "ATGAAGAAATCGAAAAAAATTTTGTTGACCATGGGAGGGATACTTCCCTTGGTTATTTCAGCGCCATTAGTTGCTGCTGGATGCAAGGATCCAAACAAACCAGAAGTTAAACCAGAGGATTCAAAGAAACCAGAAGGTGAAAAACCCGGAACAACTCCTGAAAAGAAGCCTGAAGTTAAGCCGGAGGCTGAAAAACCCGGAACAACTCCTGAAGTTAAACCAGGATCTAACAGTGATTTAAATGATTTAACAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAGATTTCAATGTTTGACAAATTAAACAAAGAAAACTTGTCAAACAACTTTGATAAAAAATTCAAAGTAGAAATTTTGAAATTAGAAGTAGATAAAAAAATTAATTCAATGTTAATTACATACAACATTTCGTTCAATGACGGAAGTAAAGACGCTAAATTAGAAAATCAAAAATATTCTTTGACAGGACTTCCAACAAACAATGAAAGCTATTTAAATGGATTATTAAACGATAAGGATACAGTATTCAATTTAGGTGATGCTTCTAATGATGATAGTTTAACAACAGTTGAACAGTTAGATGCATATTATTTAGTTGATGGTCAAGGCTCTATTAGTTTATTGGCATCTAATAAAAATGTTTATGTTCCCAAAGATCTAATCTTAGAATATACTTGA", "aa": "MKKSKKILLTMGGILPLVISAPLVAAGCKDPNKPEVKPEDSKKPEGEKPGTTPEKKPEVKPEAEKPGTTPEVKPGSNSDLNDLTEAVKKANFQYKDIDKISMFDKLNKENLSNNFDKKFKVEILKLEVDKKINSMLITYNISFNDGSKDAKLENQKYSLTGLPTNNESYLNGLLNDKDTVFNLGDASNDDSLTTVEQLDAYYLVDGQGSISLLASNKNVYVPKDLILEYT", "aa_hexdigest": "dea8d3db81b91500c3d90b9dd7829642", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 30309, "stop": 31304, "observations": { "mutation": [ 30999 ], "stop": [ 30999 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D5ADF", "query_cov": 1.008695652173913, "subject_cov": 0.5686274509803921, "identity": 0.914, "score": 354.0, "evalue": 2.82e-119, "gene_start": 1, "gene_end": 230, "reference_start": 1, "reference_end": 232, "reference_sequence": "MKKSKKILLTMGGILPLVISAPLVAAGCKEPNKPEVKPEDPKKPEGEKPGTTPEVKPEDPKKPEGEKPGTTPEVKPKSNSDLNDLTEAVKKANFQYKDIDKISMFDKLNKENLSNNFDKKFKVEVLNLEVDKKINSVLITYNISFNDGSKDAKLENQKYSLTGLSTNNESYLNGLLNDKDTVFNLGDASNDDSLTTVEQLDAYYLVDGLGSISLLASNKNVYVPEGLMLEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSINSFKEGDIEQTLKNNFIAKFEKSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVSGFKGVEKVDDEQVKKIVDKLTFGDVYFPEKIKDF" }, "paralog": false, "identity": 0.9219219219219219, "score": 563.0, "evalue": 5.64e-204, "description": "Internal stop codon at 30999. Nonsense mutation around 30999.", "psc": { "uniref90_id": "UniRef90_UPI00215D5ADF", "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI00215D5ADF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D5ADF", "UniRef:UniRef50_UPI00215D5ADF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D5ADF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5ADF" ], "product": "variable surface lipoprotein" } }, "id": "AHCKFKLKGB_93", "locus": "GCA013367975_00053" }, { "type": "cds", "sequence": "contig_1", "start": 31701, "stop": 32135, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCCGCAACATTAAAATTAAACGGCAAGGAATATAATGTTGAAACAACATTAACTCATGAATTAAAATTAGATGAATGGGTAGATGAAGTCGGAAAAGAAATTGATGGGGATTCAAATACCATTCCCGTAAATGAATTTTCTAATAAAAATTTAAAATTGAATAAAAAAATTAATGACTTCGTCAATGGTTTAGACTTTGTTCAATTAAATTCAATAAAAAGAGCAAAATCTTCTGAGGCAGAAAAAAAATTGAATTTAGAAGGTAAGGCAACCTTAATTTATGAATTTAAAAATATATATGATAATTCAACATATAGAAAAGAAGTCGTATTCGAAAATCTAAAAAAAGCAGAAAAAGTTGATTATAAAAAACAATATGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTACTAATAATAAATGA", "aa": "MSATLKLNGKEYNVETTLTHELKLDEWVDEVGKEIDGDSNTIPVNEFSNKNLKLNKKINDFVNGLDFVQLNSIKRAKSSEAEKKLNLEGKATLIYEFKNIYDNSTYRKEVVFENLKKAEKVDYKKQYASLIQKINDKKIITNNK", "aa_hexdigest": "0792fe4b7b4a9cbc4e3bc91e3af5b33c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16665.739799999996, "isoelectric_point": 8.857198524475098 }, "id": "AHCKFKLKGB_94", "locus": "GCA013367975_00054" }, { "type": "cds", "sequence": "contig_1", "start": 32277, "stop": 32405, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATTTTGGAGATAATAACAGAACTAAAGAATCATTCAAAGATTTAAAATTTGACAATGATTTTCTTGAAGCAATAAAAAGTAAAACATTAGTTAATGTTGTTAAGGATTCTGGACATGGCGAATGA", "aa": "MHFGDNNRTKESFKDLKFDNDFLEAIKSKTLVNVVKDSGHGE", "aa_hexdigest": "54e21af4c5a1e3e00801c1a41b93c3b8", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4812.288900000001, "isoelectric_point": 6.043542289733887 }, "id": "AHCKFKLKGB_95", "locus": "GCA013367975_00055" }, { "type": "cds", "sequence": "contig_1", "start": 32613, "stop": 33563, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H1", "UniRef:UniRef50_UPI00215D9390", "UniRef:UniRef90_D1J7H1", "UniRef:UniRef90_UPI00215D9390" ], "nt": "ATGGTTAAGAAAAAAGTATTATTTAAAATATTTGCTATGGGTACTATTCTAGCCGGAGGCGTTTTTAGTTTAAATTCATGTATTTTTAAATCAAATGATACAGAAAATAAAGATAATAGTTTTGAAAATAACACTAATAACGGTCAAAGCAACCAACATGATTTAGGCACCGAAGATAATAATTCAAGAAATTTGATAGTTCCTGAATCAAATAATAAGCCCGAAAATGATTCTATCCCTCAACCTGAATCAAAACCAAATATTACCCCAACGCCCGAACCAGCGCCTGAGCCAGCGCCTGAAATAAAGCCTGAACCAGCGCCTGAGCCAACTCCCAAACCAACGCCCGAACCAAAAGAAAATGATCATAAAGCCAATCATAATCAAACTCATTTTGTAAGGCCACCTTTTATTGCTAAATTTAAAATTAAAAATACTGATTTTTCAATAGCGACAATTTTTAACCATTTAGATAGTCCTGGTTCTAGAAAGAAAGAAAAATACATTGAACCAAAACCATCGATTTTATTGGCTGATAGTTCAAATAAACAGTCGGATTTGAATAAACAAGGAGCCCAAGAAGTAAGCGAATTTCTAGGCTTAATAGATGTTATTAATGAAAACAAAGATTTGGCCAATTTTGTTATTTATGGTGGCGATACTAATATAAAAAATGAAAATTTTTATTTAGCAAGAAAATATCCCGAAAATATTCAATTTACTTTAGATAGAAACTTAAAATTAAAGAAATATGACCAAAAATTTCTAACATCATTAGGAACAAGGGGTGGATATTCAGAACAATACGACAAAATGTTCTTTATTAATCAATTGCCCAATACATTTAAACCAAAAATAATTACCAATGGTCTTTTACCTTATAAGGTTGATATTTATAGAACGTTTAATGATGTTATTTCAAAAGCAGAACTTGAAAAATTAAAAGGATGA", "aa": "MVKKKVLFKIFAMGTILAGGVFSLNSCIFKSNDTENKDNSFENNTNNGQSNQHDLGTEDNNSRNLIVPESNNKPENDSIPQPESKPNITPTPEPAPEPAPEIKPEPAPEPTPKPTPEPKENDHKANHNQTHFVRPPFIAKFKIKNTDFSIATIFNHLDSPGSRKKEKYIEPKPSILLADSSNKQSDLNKQGAQEVSEFLGLIDVINENKDLANFVIYGGDTNIKNENFYLARKYPENIQFTLDRNLKLKKYDQKFLTSLGTRGGYSEQYDKMFFINQLPNTFKPKIITNGLLPYKVDIYRTFNDVISKAELEKLKG", "aa_hexdigest": "b8589887cb4b73f306d989f3cde0c469", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00215D9390", "query_cov": 0.8829113924050633, "subject_cov": 1.021978021978022, "identity": 0.91, "score": 431.0, "evalue": 4.45e-150, "valid": true, "uniref50_id": "UniRef50_UPI00215D9390" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9390", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9390" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 32613, "stop": 33866, "observations": { "mutation": [ 33561, 33732 ], "stop": [ 33561, 33732 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7H1", "query_cov": 1.0411392405063291, "subject_cov": 0.6553784860557769, "identity": 0.866, "score": 450.0, "evalue": 3.13e-154, "gene_start": 1, "gene_end": 316, "reference_start": 1, "reference_end": 329, "reference_sequence": "MTKKKVLFKIFAMGAILAGGVFSLNSCIFKSNDTENKDNSFENNTNNGQRNQHDLSTEDNNSRDLIVPESNNKPENDSIPQPESKPNIIPTPEITPKPAPEITPEPTPKPAPEITPEPTPKPAPEITPKPNENDYNKANHNQTHFVRPPFIAKFKIKNTDFSIATIFNHLDSPGTRKKGEYIEPKPSILLADSSNRQSDLNKQGAQEVSEFLGLIDVINENKDLANFVIYGGDTNIKNENFYLARKYPENIQFTLDRDLKLKKYDQKFLTSLGTSGKYSEQYDKMFFINQLPNTFKPKIITNGLLPYKVDIYRTFNDVISKAELENLKGWKSKNNDKSTIRSQISDHAPVFTDVVFSNPIETLKVNESLRKNQTLSKEQNTIRIAHWNILNFGGPNQDAKTMAIATTIYKSGFDIIGLTEINDGRGNYVQNIVDLLNEYAGKERYKFVLQNKKDTNIRPEYLQSNKFGKAQQEQVAVIYDSQNFEFVSSRSYLKPIKYYKDN" }, "paralog": false, "identity": 0.8325581395348837, "score": 619.0, "evalue": 1.97e-223, "description": "Internal stop codon at 33561, 33732. Nonsense mutation around 33561, 33732.", "psc": { "uniref90_id": "UniRef90_D1J7H1", "uniref50_id": "UniRef50_D1J7H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7H1", "UniRef:UniRef50_D1J7H1" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35745.8251, "isoelectric_point": 7.70795841217041 }, "id": "AHCKFKLKGB_96", "locus": "GCA013367975_00056" }, { "type": "cds", "sequence": "contig_1", "start": 33777, "stop": 34082, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B017", "UniRef:UniRef90_UPI00215DB2CF" ], "nt": "ATGGCTATTGCTGCCACAATTTATAAGTCCGGTTTTGATATAATAGGCCTAACTGAAATTAATGACGGAGGAGGAAACTATGTGCAAAATATAGTTAATCTTTTAAATGAATATGCAGGAAAAGAAAGATATAAATTTGTATTACAAAATAAAAAGGATACAAATATAAGGCCTGAATATTTACAATCAAATAAGTTTGGAAAAGCACAACAAGAACAAGTTGCAGTGATTTATGATAGTCAAAATTTTGAATTTGTTTCAAGCCAAAGCTATTTAAAACCAATTAAATACTACAAAGATAACTAA", "aa": "MAIAATIYKSGFDIIGLTEINDGGGNYVQNIVNLLNEYAGKERYKFVLQNKKDTNIRPEYLQSNKFGKAQQEQVAVIYDSQNFEFVSSQSYLKPIKYYKDN", "aa_hexdigest": "bdd71c7195bfed73066c3c222445f6b4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00215DB2CF", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.941, "score": 187.0, "evalue": 6.47e-60, "valid": true, "uniref50_id": "UniRef50_A0A449B017" }, "pscc": { "uniref50_id": "UniRef50_A0A449B017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B017" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11601.896499999995, "isoelectric_point": 7.838082313537598 }, "id": "AHCKFKLKGB_97", "locus": "GCA013367975_00057" }, { "type": "cds", "sequence": "contig_1", "start": 34675, "stop": 35103, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATGCAATTGAAAAAATTAAATCAGCATTTAGCTTTAATGATGTTTTAGTATTGTCTGAATTAGTAGGTGAAGATAAACCAATCAATGAAACTGCTTTGGTTAAATTAATCAATTTAGTATTTGGAAAAAGTAATTTTAATAACTCTGTAATCTATAATGCATTAAGAAATCTAAACAATAATTCAGACGAATCAAAACGTTCAAACATGAATACCTTAAATAGCTCGGTTTCAAATGCGTTGGGAAGCATTTTCAAATCTTCTTCAAAAGCTAGCGATAAAGATGACCCTGATAATATTGAATATTCAGCAGACGTTTTAAAATTACTTGATACAATTTATAAATTGCTTGCAAAGGCATACAATAATGATAAAGATGCAACTTCACCAACATATAAAGTTAGGGCACAATCTGAAGCATGA", "aa": "MNDAIEKIKSAFSFNDVLVLSELVGEDKPINETALVKLINLVFGKSNFNNSVIYNALRNLNNNSDESKRSNMNTLNSSVSNALGSIFKSSSKASDKDDPDNIEYSADVLKLLDTIYKLLAKAYNNDKDATSPTYKVRAQSEA", "aa_hexdigest": "691cc1cd09a79762d24b4548ce21e42b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15646.26329999998, "isoelectric_point": 5.163902091979979 }, "id": "AHCKFKLKGB_98", "locus": "GCA013367975_00058" }, { "type": "cds", "sequence": "contig_1", "start": 35104, "stop": 35511, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTAATAAGTAAAAAATTCATTGATTTTCCAGAAATTAAAAACCTAATTGAAGCCGTAGTTAATGATTTTCTAGCAGGAGCAAAAATTGAATCAAGCAACCTTGGGGACTTAATATCTAAAACAATTACTAGAATTCAAGATAATCTAATTAAAAACTCTTCTGAACTTATTAAGCATCTTGCAACTGATGATGAAATTACAAATCACATTGTTGAAAGTATTTTAAATTATCTAAAAATAGAAGGAAATGATGAAGATAAAAAATTCCTAAAAGACTTAATTGGAAAAATCGTGCCAGCATTGTTAAATAATGATTTTATTAAAAGGAAATTCATTAAACGTTCAATAGGATTGATAAGCAATTATGCTAAACAATTTGATATTTTAAATCCTGTTAAATGA", "aa": "MELISKKFIDFPEIKNLIEAVVNDFLAGAKIESSNLGDLISKTITRIQDNLIKNSSELIKHLATDDEITNHIVESILNYLKIEGNDEDKKFLKDLIGKIVPALLNNDFIKRKFIKRSIGLISNYAKQFDILNPVK", "aa_hexdigest": "116e63efe0a447f507f6953747629ce4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15354.683099999978, "isoelectric_point": 6.7399904251098635 }, "id": "AHCKFKLKGB_99", "locus": "GCA013367975_00059" }, { "type": "cds", "sequence": "contig_1", "start": 35548, "stop": 36237, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGTAAATAAAGATAAATTTAAAGCATTTATTAAAAACGCAGTAAATGAAACGACTTCTAACAAAAACTTAATTAACAAATCTATTGAATATGCATTGAATTTATTCAACAAGAAATATTCAATTAGTTCAAGTAGCGCTCAAATTCAAGGAATTGCAAATCTTTTGACTAGATTAATTAACAAACTAGGTTCAATGAACGTATTTAGCAATATATTGGGAGACTTCATTGATTTGTTTGTTGATGTTGATTTACTTGACAATAATGGTCATTTCAATGGAAATGCTTTGGCATCCAAACTTCTTGAATCTATTAAAAAGATTGAATACCATAACTATTTAACAACAGAAAATTTAAGTGAATTATTTGAAACAATATTAGATAAAAATATTGATAAAAATGTCTTGTATAATGAATTGAATTTCGCTTATTCATATATAGTTGATAATTTTGATAAATTTAAACAAGCATTCTCTGCTAAAAGCAATTCTACAACATTCAACGAAGTAGATAAAACTAAACAAGATAAGGAACACGAACAATTCCTAAAGAATTTAGAAAAATTGATTTACAATGCTTTAAAAGCTCTAAATGGATCAATAAAAGCAGATAATAAAAATGGAAAAGAAGCATTAGTTGATTTTGTATATGATGTTTTGAAGGATCAAATTAAGAAATTTAATTGA", "aa": "MSVNKDKFKAFIKNAVNETTSNKNLINKSIEYALNLFNKKYSISSSSAQIQGIANLLTRLINKLGSMNVFSNILGDFIDLFVDVDLLDNNGHFNGNALASKLLESIKKIEYHNYLTTENLSELFETILDKNIDKNVLYNELNFAYSYIVDNFDKFKQAFSAKSNSTTFNEVDKTKQDKEHEQFLKNLEKLIYNALKALNGSIKADNKNGKEALVDFVYDVLKDQIKKFN", "aa_hexdigest": "00749aa281b27e4f7d1861064b744dd4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26155.33500000003, "isoelectric_point": 7.748143577575684 }, "id": "AHCKFKLKGB_100", "locus": "GCA013367975_00060" }, { "type": "cds", "sequence": "contig_1", "start": 36277, "stop": 36567, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAATGCGTATTTTCAATATTAGATAATAAAGCAAAGAAAGAAATTAAAGATGAATTAAAAAATTCAGTTGATATTTTCTTGCAAAGTAATAAATTCAAGCAATATATCAAACACAATTTATTAAAAATAAAAGAAAAAATTCTAAAAATTGATTCAAAAGCTTCTGAATTAGGCAATGAAATAGTTGAAACATTAATGAAGGTGATTTTTGATTCAAACAATACAAAAGAATTTATACTAACATTATTGAATTCATTTATAGATTTAGGCGATAACAATCAATGA", "aa": "MFKCVFSILDNKAKKEIKDELKNSVDIFLQSNKFKQYIKHNLLKIKEKILKIDSKASELGNEIVETLMKVIFDSNNTKEFILTLLNSFIDLGDNNQ", "aa_hexdigest": "388e7ab597102b89ff2fd9b22dd99354", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11167.919499999987, "isoelectric_point": 8.644839668273928 }, "id": "AHCKFKLKGB_101", "locus": "GCA013367975_00061" }, { "type": "cds", "sequence": "contig_1", "start": 36829, "stop": 37014, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAAATCTAAAGAATATTACATGGCCTGTTAATACAAAAGAAACATTACTAAAATTAATTTCGCTAGGCATTGAAAAATTGCCAGAATTTAATATTCTAGATGGCTTAATTGAAAATGTTTTAGACTACTCTGTCAATAATCTAAACAAGAATCAATTTGAATTTAAAAAGTACGATTGA", "aa": "MEQNLKNITWPVNTKETLLKLISLGIEKLPEFNILDGLIENVLDYSVNNLNKNQFEFKKYD", "aa_hexdigest": "efb98cd7c5a9087269a8f137c7c42598", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7184.179400000001, "isoelectric_point": 4.964397239685058 }, "id": "AHCKFKLKGB_102", "locus": "GCA013367975_00062" }, { "type": "cds", "sequence": "contig_1", "start": 37564, "stop": 38103, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACCAGAAGTTCAAAAGTTATTAAAAAATGAAAATTTAAAACAATATTTAACAAAAATAATTAATAATGAGTTAAAAAATGTTCATAATGAAGTTCTAAATAAAGTCAATACAAATGATAATAATCTTGCCAATTTTATTGTCGATAAATTCGATAAGATTATTGATAATAAATTAATTGACAAAGTTTTCGAATTTATTGAAAATTTAATTGAAAACGGCAGTGAATATGATGCTCAAACATTGCCCGAATTTATCAATAAAATATTGAAAAATCTTGCAGACAACAAGGGCTTTGATGCGTTAGAACAATTGCTAGAAAATGTTTTATCAAATAAAGATATAACCCAAAAATTAGTTGAATTTTTAGTTGCGTTTTTAGAAATTGAAGCAAAAACAAAATTGAATTCTGATGAAGTTAACCAATTAACTAATTATTTCAACAAGATTTTACCTACTTCATTGAAATCTAATTTATATAAAAACTTAAAAATTAAATTAACAAATGAATTGAAGTCAATAAATAAATATTCTTGA", "aa": "MKPEVQKLLKNENLKQYLTKIINNELKNVHNEVLNKVNTNDNNLANFIVDKFDKIIDNKLIDKVFEFIENLIENGSEYDAQTLPEFINKILKNLADNKGFDALEQLLENVLSNKDITQKLVEFLVAFLEIEAKTKLNSDEVNQLTNYFNKILPTSLKSNLYKNLKIKLTNELKSINKYS", "aa_hexdigest": "3805f0f450217144224ea3a7cb056fad", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20805.651400000028, "isoelectric_point": 5.974426078796387 }, "id": "AHCKFKLKGB_103", "locus": "GCA013367975_00063" }, { "type": "cds", "sequence": "contig_1", "start": 38143, "stop": 38337, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGATACATTAAATGAAGAAAATGTAAAAACATTATTTTTGATATTAATCAAGAATTTAAATGAATTTGCAAATGTTGTTTTAGCAAACAACGACACAACAACACAAAAACAAAATGAAGGTTCAAAACCAATTAAATTAGACAATGATTTAAATTTTGAGCAAATCTTCTTACTAATTAAAAAATTATGA", "aa": "MIDTLNEENVKTLFLILIKNLNEFANVVLANNDTTTQKQNEGSKPIKLDNDLNFEQIFLLIKKL", "aa_hexdigest": "d583c3f50b1ec02d8d956186b7985824", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7376.437099999998, "isoelectric_point": 4.853277015686035 }, "id": "AHCKFKLKGB_104", "locus": "GCA013367975_00064" }, { "type": "cds", "sequence": "contig_1", "start": 38788, "stop": 39111, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAACTATTTAAAATCAATATTTAGTGACTACTTCAACATTGAAAAAAATAAGGATTTAATTAATAATTTTGTTGATTTACTACTCGAAAGAGATGATAAATCAGAAGGAATAAAATTTTCTGAATTAATAAAAACACTTTCTGTTCTTGCTTCTAAATCCGAACTTGTTAACTACGTCTTTAAAAAATTTGACATTAAATCTATTATATTAAATTACTTGGATTCATTTAAAGACAACAAACTATCTAACGATGCTGAATTAAATTCACTATTACTAACTTTAATCACCAAAACTAGAAATTACATAGATAGTCAATGA", "aa": "MKNYLKSIFSDYFNIEKNKDLINNFVDLLLERDDKSEGIKFSELIKTLSVLASKSELVNYVFKKFDIKSIILNYLDSFKDNKLSNDAELNSLLLTLITKTRNYIDSQ", "aa_hexdigest": "8005ac58b7e2476b859c89f79a40ea85", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12511.222799999978, "isoelectric_point": 6.255949592590333 }, "id": "AHCKFKLKGB_105", "locus": "GCA013367975_00065" }, { "type": "cds", "sequence": "contig_1", "start": 39523, "stop": 41664, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATCTATTTGGTGATTTTTCTAATAAAAAATTACAAGAGCTAATTAAAAATATAAAACAAGCAAACCTGATTACAATTTCTTTGGGATTCAATGACCTTTTTGAACAATCATCATTTTTTAAAGAAGCGTTTGATCTTTTAAGAGAAAATAAAAATAAAGAAGAAATTTTGAAATATTTTTCAAAAATTCTTCTTGAATTTAAAAGAAATTGTAAAAATTTTGAAGATTCATATTCAAAATTAATTTCAATCATTAAACAATATAATCCTAAGGCAAATATTAATCTTGTGGGTTATATATCGCCTTATTTGAAATTAACAAAATTAATTGACAAGCAATACAATATTTCATTGACTAATGATTGCATAGAAGAATTAAACAAATCATTAGCAAACATTGCCAAAACAAACAAGCTAAACTATTTAAACATTAATAATGACATTAAAAATCTCAAAGATAATGCCGATAAACTAAACAATAATTTCTTTGACTGCTTCCCATCTAATAATTTATACAAAAAATTAGCGCAAGATATTTTTATGAAAATGTCATTGCCTAATAATAAATATAAAGAATTAGTTGATAATAATTCTAATCTAAATGAATTTAGCCAATCACTTGAATTTAGCAAAACTCCTTCCGAAATTTTTTCAATGATTTTAGGAATAAATGGCAACAATGTAGATTCTTTTGAAAAAGAATATACATTCGAAGAAAATGCAGATATCAAAAATATTAATTCTTCATACAATTCAAACCATATAATCCTTCAATTAAAATCAATGTTAAATTCAAACAAAAACATTGAAGATAAAGAAATTTTGAATACATTTTCAATACTATTCGGATTAATAGGCTTAGACCCTAAAGATTTAAACAGCTTTAATAAGTGTTTATTAGAATCTTTAAAGAATAAAGAAAGTAAAAAATCTATTATTAATTTTTTGAATTTCTTTATTGATTCAAAAAATTTAAGCAAATCGTTTCAAGATATCAACAATGCTTTAAATAATGCTATTAATAAAAAATCATATGCAGGTCTAAACTTTGAAGAAATTAAAAAAATTGTTTATTCAACAACTACTTCTAAACAAAATATTTATGAAATTTTAAAAGAAATTTTCTATAACAAATCTTTAAGTCAAGATCTTAAAAGAAGTGAATATGCAAAATTATTTAAGGCTATATTTTCAGATGTATTCATTAAAAAAATATTAAAGAGATTATTGCCTCAAACTACTATTGATTTATTCAAAAATCTTTTTAATAACAAAGATATTGAAAAAGGTTTAGACACCATTTTTCAAAAAGTGTTGAATTTGTTAATTAATAATTCAGATAAATATTTTAAATTTGAAAAAATTGATGACTTTATTACTACATTGGTTCATGATTCAAGCAAAGATGTAAATGATTTAGTTAGCCTAATAATTAAAGAAATTTCAAAAGATAGAAAGCTTGCAAACTACTTGACCGGAAAAATTGTTGATTCTTTATACAAATCATATGATTTAGATTTACTTGAAAAACCAAATATTTCTTTATTTGTTTCAACATTTTTTGACAACATTTCAAACTTCGATGGTTTATCTCAAATATCAAACATTATTATTCAAATATTAATGAATTCTAAACTAGATGATGACCACCAAAATAGGGATTTAATTTCAGAAATTCTGAATGGAATTCTTTTTGATAAATACGACCTAAATAGGGATAACTCGCTTTTATTTAAAATCCTATCGTTTGAACCAAAAATACAAAATATTAACGACTACAAACAAGAATTAAAACATTTAGCCCTAAAATATGCAATTAAAAATAATGCATTAATGCCAAAAAACATAGGCAAATTAATCGATAAAGATTCAAGAGAAAACATATTAAGATTTTATAAAAATATAGCATTAAATGCAAAGAAGAATTTAAACGAATTTGGTAGCGAAATATTTAAAGATATTATCTCGCTTCTAGTAAATGAATTATTTAAGAATAATAGTTTGTTCAGCCAATTAATTGATCAAATTGGAAATTATGTTGTTATTCAGCCTATCGTTGCTTTCATTAAATCAATTAATTGCAAAAAGATATTCAACAATGAAGAAGAAATTCAAAAATTTGTTGAAAATGTTTGA", "aa": "MNNLFGDFSNKKLQELIKNIKQANLITISLGFNDLFEQSSFFKEAFDLLRENKNKEEILKYFSKILLEFKRNCKNFEDSYSKLISIIKQYNPKANINLVGYISPYLKLTKLIDKQYNISLTNDCIEELNKSLANIAKTNKLNYLNINNDIKNLKDNADKLNNNFFDCFPSNNLYKKLAQDIFMKMSLPNNKYKELVDNNSNLNEFSQSLEFSKTPSEIFSMILGINGNNVDSFEKEYTFEENADIKNINSSYNSNHIILQLKSMLNSNKNIEDKEILNTFSILFGLIGLDPKDLNSFNKCLLESLKNKESKKSIINFLNFFIDSKNLSKSFQDINNALNNAINKKSYAGLNFEEIKKIVYSTTTSKQNIYEILKEIFYNKSLSQDLKRSEYAKLFKAIFSDVFIKKILKRLLPQTTIDLFKNLFNNKDIEKGLDTIFQKVLNLLINNSDKYFKFEKIDDFITTLVHDSSKDVNDLVSLIIKEISKDRKLANYLTGKIVDSLYKSYDLDLLEKPNISLFVSTFFDNISNFDGLSQISNIIIQILMNSKLDDDHQNRDLISEILNGILFDKYDLNRDNSLLFKILSFEPKIQNINDYKQELKHLALKYAIKNNALMPKNIGKLIDKDSRENILRFYKNIALNAKKNLNEFGSEIFKDIISLLVNELFKNNSLFSQLIDQIGNYVVIQPIVAFIKSINCKKIFNNEEEIQKFVENV", "aa_hexdigest": "60347b83cdf092e261eb1eec43c6335d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 82877.19560000044, "isoelectric_point": 8.694222450256348 }, "id": "AHCKFKLKGB_106", "locus": "GCA013367975_00066" }, { "type": "cds", "sequence": "contig_1", "start": 41764, "stop": 42132, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGTACAAAAATCCTCAAGGCAATTGTTGCTTTAGGAATTACATCTTCAATTGCTTCTAGTTCATTAGTTGCTATATCATGCAAAGACAAAACTAAGAAGCCAGATATAAAAATAACTCAAAGCGAAAATAAAGTTTCTAAAAAAATTAATTATGTTGCATTAGGAGACGGCTTTGCTGCCGGTTATAATAGCAGCTCAAATTACTATTTCAACAATGAATATGATAGCAAAAACGATTTAGTTTTAGGAACATCGTATCCTTCATACATTGCTAATTTTATAAAACTATTATCTGATAAACAAACAACATTATCAACTTATTATAACTACGGTTTGCCACTTTCAAATGTTAATGATTGA", "aa": "MKKSTKILKAIVALGITSSIASSSLVAISCKDKTKKPDIKITQSENKVSKKINYVALGDGFAAGYNSSSNYYFNNEYDSKNDLVLGTSYPSYIANFIKLLSDKQTTLSTYYNYGLPLSNVND", "aa_hexdigest": "1f682091f6ea34d067179224de14d746", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13373.013699999985, "isoelectric_point": 9.320919609069826 }, "id": "AHCKFKLKGB_107", "locus": "GCA013367975_00067" }, { "type": "cds", "sequence": "contig_1", "start": 42142, "stop": 42696, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAACAAGATCAGCAGGTTTTTCAACTGTAAATTATTATAATTTTACTCAACCAACAATTATGGTTCATGGTATCTTAATGTTTATTGGATTTTCTCCTGTTTCAACTGCCGGAGGTATTCGTAATATTACAGTGGCTATAATTTTTATGAGTATAATTACTTTAATTAAAAATCGCAAAAACATAAATGCCTTTCATAGACAAATAGGAAAAGAATCTTTAATTAAGGCAGTTAACATTTTTGCAATTGCAGTTTTTATAACTCTAATAATGACAATAGTAATATATCATACACTTCCAAGCGATTTAAAAAATGTTTATAATGAGTCATATCCAATGGTATATGTAATGTTCGAAGTTTTCTCAGCTTTTGGAAACTCAGGACTTAATATTGGATTAACGCCAAATTTGGGCATTACTGGAAAAATACTTTTAATTATAACAATGATAGTAGGACAATTAGGAATATCACAATTTCTAAAAATTTTTACTTTTTCAAAATCAAAACCACAAAGATATCAATATATTTATGAAGATATTTCTATGGGTTAG", "aa": "MSTRSAGFSTVNYYNFTQPTIMVHGILMFIGFSPVSTAGGIRNITVAIIFMSIITLIKNRKNINAFHRQIGKESLIKAVNIFAIAVFITLIMTIVIYHTLPSDLKNVYNESYPMVYVMFEVFSAFGNSGLNIGLTPNLGITGKILLIITMIVGQLGISQFLKIFTFSKSKPQRYQYIYEDISMG", "aa_hexdigest": "e672b8f167eea24e527da42b8b3b7da1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20572.1435, "isoelectric_point": 9.800693321228025 }, "id": "AHCKFKLKGB_108", "locus": "GCA013367975_00068" }, { "type": "cds", "sequence": "contig_1", "start": 42907, "stop": 43080, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACTAAGATTTGGAATATTTCATACACTAAGCGCAATAAATAATGCTGGATTAGATATAATTGGCACAAAGAGTTTGCAACCTTATTATCATAATTATTACTTTTTATTTTTAACATCAATTTTATTTCTAATTGGTGGAATGGGATTCCCTGTATTCTATGATGTTTGA", "aa": "MSLRFGIFHTLSAINNAGLDIIGTKSLQPYYHNYYFLFLTSILFLIGGMGFPVFYDV", "aa_hexdigest": "aaf7ace7f181f8eeb3756d7d54f88553", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6498.521599999998, "isoelectric_point": 6.68991527557373 }, "id": "AHCKFKLKGB_109", "locus": "GCA013367975_00069" }, { "type": "cds", "sequence": "contig_1", "start": 43132, "stop": 43515, "strand": "-", "frame": 1, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F33CDF9", "UniRef:UniRef90_UPI001F33CDF9" ], "nt": "TTGCTGTTAGCAGCTTCATCATTTACAACAACCGGTTTAAGTGGAGTTGCCGTTAGTGAAGTATTTAACATGTTTGGACAGATATTAATAGCTGTCTGTTTTATTCTTGGCGGAATTGGAATTTTTACATTTAAACTTTATATTTTTCAATCATTACTTGGATTAAGAACGAATTTATTTAGTGGCCAAATTCATCAAACTGAAAGAGGCGGTTCTAATATTTATGAAACAAAAAAAATGATAAAAATTTCAATAAGTTTTTTAATTATTATTTCTATATTATTTTCTTTAATATTTTCTTTAATATTTTATTATGATAATACAGCACTTTTTAGTGATGAAAAGATGTCTCAATTAAATGCAATAAGCAAAAATAATCCATGA", "aa": "MLLAASSFTTTGLSGVAVSEVFNMFGQILIAVCFILGGIGIFTFKLYIFQSLLGLRTNLFSGQIHQTERGGSNIYETKKMIKISISFLIIISILFSLIFSLIFYYDNTALFSDEKMSQLNAISKNNP", "aa_hexdigest": "58648bd2bfc441465325427d11b9e690", "start_type": "TTG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "both", "pseudogene": { "start": 42831, "stop": 43572, "observations": { "insertion": [ 42903, 42928, 42974, 43005 ], "mutation": [ 42909, 43134, 43563 ], "stop": [ 42909, 43134, 43563 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F33CDF9", "query_cov": 1.0, "subject_cov": 0.5059760956175299, "identity": 0.976, "score": 214.0, "evalue": 3.59e-68, "gene_start": 1, "gene_end": 127, "reference_start": 20, "reference_end": 146, "reference_sequence": "MLYWEVSHNPSKHISYLDALLLAASSFTTTGLSGVAVSEVFNMFGQILIAVCFILGGIGIFTFKLYIFQSLLGLRTNLFSGQIHQTERGGSNIYETKKMIKVSISFLIIISILFSLIFSLIFYYDNTALFSDEKMSQLNALSKNNPWTFNPYEESIRNPYHNFGMSLRFGIFHTLSAINNAGLDIIGTKVCNLIIIIITFIFNINYISNWWNGIPVFYDVWQKLKASRKTILVIALHYLQNLQLLHTFLHH" }, "paralog": false, "identity": 0.9755102040816327, "score": 427.0, "evalue": 1.33e-154, "description": "Internal stop codon at 42909, 43134, 43563. Frameshift due to insertion around 42903, 42928, 42974, 43005. Nonsense mutation around 42909, 43134, 43563.", "psc": { "uniref90_id": "UniRef90_UPI001F33CDF9", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI001F33CDF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F33CDF9", "UniRef:UniRef50_UPI001F33CDF9" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F33CDF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F33CDF9" ], "product": "potassium transporter TrkG" } }, "id": "AHCKFKLKGB_110", "locus": "GCA013367975_00070" }, { "type": "cds", "sequence": "contig_1", "start": 43842, "stop": 44534, "strand": "+", "frame": 3, "gene": "trkA", "product": "Potassium uptake protein KtrA", "db_xrefs": [ "COG:COG0569", "COG:PT", "RefSeq:WP_114090906.1", "SO:0001217", "UniParc:UPI000DED9C61", "UniRef:UniRef100_UPI000DED9C61", "UniRef:UniRef50_D1J7H4", "UniRef:UniRef90_D1J7H4" ], "nt": "ATGAGTTTGTTTAAAAAGATACGTGAAATTTGTATTATTGGTATAGGTCGTTATGGTTCGGCAGTAGCAAGAACATTATTGGCCGATAGAAATAATAATATAAGGATAGTTTTAGTCGATGAAGATGAAAAACATTTGTTGCCTTTTAAAGATGAAGTAGAAATGATCTACGTTGCAGACTGTGCTGAACAAAAAACATTAGAAAGTCTTAATATAAAAGATTTTGATGCTGTTATTGTTGGTGCTTCAAATAATATTGAAATAGTTGCTGCTTTAACTGAATGTGGTGTAAAAACTATAATAGCAAGGGCTTCAACAAAAAGACATGCCCAAGTTTTAAAGCAAATTGGTGTTAGCTCAATTGTTTGTCCTGAAGAAGAAGCCGGAAAGAAAACTGCAATGTTAGTTGCAAACCCTGCGCTTGCATTATATTCAGAAAACATGGTTGAATTGCAAGACGGATTTGTTTCTGCTTCAATATATGTTAAAAACCCTGCTGTTGCAAACAAAAAAATTAGTGAATTAAATTTCAGAAATAAATACAATGTTTTAGTAACTATGATTAAGAGAGATAAACATTCATACATTCCTGAAGGCGATTTTGAAATAAAATTAGATGATTTAATAACTCTTATTGGTCAAATTGAAGATATTATTCAAGTTATTGAAATTTGCGCAATGAACGAAAAATAA", "aa": "MSLFKKIREICIIGIGRYGSAVARTLLADRNNNIRIVLVDEDEKHLLPFKDEVEMIYVADCAEQKTLESLNIKDFDAVIVGASNNIEIVAALTECGVKTIIARASTKRHAQVLKQIGVSSIVCPEEEAGKKTAMLVANPALALYSENMVELQDGFVSASIYVKNPAVANKKISELNFRNKYNVLVTMIKRDKHSYIPEGDFEIKLDDLITLIGQIEDIIQVIEICAMNEK", "aa_hexdigest": "bf4a2097f1a524c05cb02a2c3190d329", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI000DED9C61", "ncbi_nrp_id": "WP_114090906.1", "uniref100_id": "UniRef100_UPI000DED9C61", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DED9C61", "RefSeq:WP_114090906.1", "UniRef:UniRef100_UPI000DED9C61" ] }, "ips": { "uniref100_id": "UniRef100_UPI000DED9C61", "uniref90_id": "UniRef90_D1J7H4" }, "psc": { "uniref90_id": "UniRef90_D1J7H4", "gene": "TrkA", "product": "Potassium uptake protein KtrA", "uniref50_id": "UniRef50_D1J7H4", "cog_id": "COG0569", "cog_category": "PT" }, "pscc": { "uniref50_id": "UniRef50_D1J7H4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H4" ], "product": "Potassium uptake protein KtrA" }, "genes": [ "ktrA", "trkA" ], "id": "AHCKFKLKGB_111", "locus": "GCA013367975_00071" }, { "type": "cds", "sequence": "contig_1", "start": 44645, "stop": 44854, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTTAGACAATGAGCTTCTTAAAAAATTAGAAGCAAAATACGAATCTAATTTAAATAACAAAATTATTGAAAATGCTATTTTTAAAAATGGTATTAAAGCTTCATCAACAAATAATCAAGTTGTAAGAAAACACGATTTTCAATTTTCTGTTGAAGTAAAACCTGGCTCAATAACTAACCAAAAACATTCAGGTAGATGTTGA", "aa": "MAILDNELLKKLEAKYESNLNNKIIENAIFKNGIKASSTNNQVVRKHDFQFSVEVKPGSITNQKHSGRC", "aa_hexdigest": "3609b43cf55c2d29729f13c1fda61b8a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7802.815200000001, "isoelectric_point": 9.463910484313967 }, "id": "AHCKFKLKGB_112", "locus": "GCA013367975_00072" }, { "type": "cds", "sequence": "contig_1", "start": 44891, "stop": 44983, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGATTTAAACATTGAAGATTTAGAATTTTCACAAAATTTTATTCATTTTTATGACAAATTAGAAAAAGCAAATGCATATTTAACATGA", "aa": "MKDLNIEDLEFSQNFIHFYDKLEKANAYLT", "aa_hexdigest": "189e783e2c094f0c78409ad88256c469", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3651.0591000000004, "isoelectric_point": 4.581245613098145 }, "id": "AHCKFKLKGB_113", "locus": "GCA013367975_00073" }, { "type": "cds", "sequence": "contig_1", "start": 44999, "stop": 45178, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTAGATAATGAAGATAGATTATTTAGAAGATATAATGAAAACCCTATTTCAGATGGTGGATATTTTGAATTCTTTTTAAATATTGCTAGCAAATATGGAATTGTTCCAAAATCTGCAATGCCAGAATCATTCCATAGTGAAGCAACAGACGATATGGTGGAACAAATTAATTGA", "aa": "MELDNEDRLFRRYNENPISDGGYFEFFLNIASKYGIVPKSAMPESFHSEATDDMVEQIN", "aa_hexdigest": "4247cef103c9ccdb1cbd7ca155fe7e68", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6879.498900000002, "isoelectric_point": 4.303871345520021 }, "id": "AHCKFKLKGB_114", "locus": "GCA013367975_00074" }, { "type": "cds", "sequence": "contig_1", "start": 45203, "stop": 45592, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGAAGCATATAAAAAGAATCATAATATGAGTGAAGTTGAAGCAATTCAACAACATGCTTTGGAAGACGTTTACAACATTTTAGTTAAGTCCCTAGGTATGCCTCCAAAAACATTTATTTTTGAATACTATGATAAAGATAAAAAATTTGTAAGATTGCCAGAAATGACACCAATAAAGTTTTTTGAAACATACATTAAACAATTTGTTGAAAATAAGGTTGATGTAATTAGCGACCCAAGAGAAATTTATCCTTACTATAGACAATTGGTTGCTCCTTCGGTTTTCAATTCGGTTGGTGGTATTGAATATAAAATGTTAAATATACCAATGAATGAATTAAAGGCAGCAGCAATTAAACAATTGCAATCTGGTCAATCAATTTGA", "aa": "MREAYKKNHNMSEVEAIQQHALEDVYNILVKSLGMPPKTFIFEYYDKDKKFVRLPEMTPIKFFETYIKQFVENKVDVISDPREIYPYYRQLVAPSVFNSVGGIEYKMLNIPMNELKAAAIKQLQSGQSI", "aa_hexdigest": "86e947b32f4a59983984df1705be817d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15117.370899999984, "isoelectric_point": 7.850975990295411 }, "id": "AHCKFKLKGB_115", "locus": "GCA013367975_00075" }, { "type": "cds", "sequence": "contig_1", "start": 45875, "stop": 45976, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATTGTTGACAAGGAATTTTTATCAAAAGAAGCAATTGAAGGTCTTTCAAAAGAAGCTATAGAAATTTCTCCATACGACCCATTATGCGAAGAATAA", "aa": "MLIVDKEFLSKEAIEGLSKEAIEISPYDPLCEE", "aa_hexdigest": "eb71dcd39b353ef260716d4fc5eb2555", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3740.2521000000006, "isoelectric_point": 4.216737174987793 }, "id": "AHCKFKLKGB_116", "locus": "GCA013367975_00076" }, { "type": "cds", "sequence": "contig_1", "start": 46089, "stop": 46232, "strand": "+", "frame": 3, "gene": "acpS", "product": "Holo-[acyl-carrier-protein] synthase", "db_xrefs": [ "COG:COG0736", "COG:I", "SO:0001217", "UniRef:UniRef50_D1J7H8", "UniRef:UniRef90_D1J7H8" ], "nt": "ATGTTAGGTGTGGACTTAACCGATATTAGACGATTTAAAGAATATAGTGAAACGCTTTTGATTAAGTTTTTACATCCGATTGAAATTGAACAATTTAAAAAGGTTTCGATAACAAAACGTGCTGATTTTCTTGCGACTAGATGA", "aa": "MLGVDLTDIRRFKEYSETLLIKFLHPIEIEQFKKVSITKRADFLATR", "aa_hexdigest": "c6a974de7e7b90d325ed84c9ef20712f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "acpS" ], "truncated": "3-prime", "pseudogene": { "start": 46089, "stop": 46416, "observations": { "insertion": [ 46380 ], "mutation": [ 46230 ], "stop": [ 46230 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7H8", "query_cov": 1.0, "subject_cov": 0.42727272727272725, "identity": 1.0, "score": 94.7, "evalue": 3.38e-24, "gene_start": 1, "gene_end": 47, "reference_start": 1, "reference_end": 47, "reference_sequence": "MLGVDLTDIRRFKEYSETLLIKFLHPIEIEQFKKVSITKRADFLATRWAIKEAIFKADNQYYSFNQINITKDEVGRYVFKDFEISTSNEGIYIIAVVLKDRTNSRSYLWI" }, "paralog": false, "identity": 0.9722222222222222, "score": 198.0, "evalue": 1.08e-67, "description": "Internal stop codon at 46230. Frameshift due to insertion around 46380. Nonsense mutation around 46230.", "psc": { "uniref90_id": "UniRef90_D1J7H8", "gene": "AcpS", "product": "Holo-[acyl-carrier-protein] synthase", "uniref50_id": "UniRef50_D1J7H8", "cog_id": "COG0736", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7H8", "UniRef:UniRef50_D1J7H8", "COG:COG0736", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7H8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H8" ], "product": "Holo-[acyl-carrier-protein] synthase" } }, "id": "AHCKFKLKGB_117", "locus": "GCA013367975_00077" }, { "type": "cds", "sequence": "contig_1", "start": 46413, "stop": 46676, "strand": "+", "frame": 3, "gene": null, "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AB411F", "UniRef:UniRef90_UPI0023AB411F" ], "nt": "ATGAATTTAAGAACAAGAATATTTTTTAGATTTTTTGGTTTGTTACATTGCATATCGTTTTTATGTGCAAAATCAAGACGTGATTTTAAAATGCCTGATTATTACAAAGTAGTAGAAAAAAACGACTTTTTGCAACAAAAAAGTAATGCCATTTTAAACCATTTCAATATTTCTGTTAATGTTGAAGGGTTTGAGAATGTTCCTAATGGACCATGTTTAATTATTCCAAACCATTCAACGTATTTAGATCCACTAATAATGTGA", "aa": "MNLRTRIFFRFFGLLHCISFLCAKSRRDFKMPDYYKVVEKNDFLQQKSNAILNHFNISVNVEGFENVPNGPCLIIPNHSTYLDPLIM", "aa_hexdigest": "e4346338aaa50bbc249342aa214d6162", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 46413, "stop": 46923, "observations": { "insertion": [ 46866 ], "mutation": [ 46674, 46686 ], "stop": [ 46674, 46686 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AB411F", "query_cov": 1.0, "subject_cov": 0.514792899408284, "identity": 1.0, "score": 182.0, "evalue": 2.14e-57, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MNLRTRIFFRFFGLLHCISFLCAKSRRDFKMPDYYKVVEKNDFLQQKSNAILNHFNISVNVEGFENVPNGPCLIIPNHSTYLDPLIMWSALWNHRDGSKQSKLVNFVARKEITKKKMVKRIADLGQTYYIDTQNPREALATLKDFGQFIKKINHAALFLLKEQEQKMEN" }, "paralog": false, "identity": 0.9822485207100592, "score": 330.0, "evalue": 5.01e-118, "description": "Internal stop codon at 46674, 46686. Frameshift due to insertion around 46866. Nonsense mutation around 46674, 46686.", "psc": { "uniref90_id": "UniRef90_UPI0023AB411F", "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "uniref50_id": "UniRef50_UPI0023AB411F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AB411F", "UniRef:UniRef50_UPI0023AB411F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AB411F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AB411F" ], "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "AHCKFKLKGB_118", "locus": "GCA013367975_00078" }, { "type": "cds", "sequence": "contig_1", "start": 46719, "stop": 47018, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAATTTCGTTGCAAGAAAAGAAATAACAAAAAAGAAAATGGTTAAAAGAATAGCAGATTTAGGTCAAACTTATTATATTGATACTCAAAACCCTAGAGAAGCATTAGCAACCTTGAAAGATTTTGGCCAATTTATTAAAAGAAATAAATCATGCGGCGTTATTTTTGCTGAAGGAACAAGAACAAAAGATGGAAAATTAGGTGAATTTCATAGTGGCGCCTTTAAACTTGCTCAATCTTCATATTTAACAATTGTTCCAGCAACAATAAATAATGCAGCAAACGCATTGGATTGA", "aa": "MVNFVARKEITKKKMVKRIADLGQTYYIDTQNPREALATLKDFGQFIKRNKSCGVIFAEGTRTKDGKLGEFHSGAFKLAQSSYLTIVPATINNAANALD", "aa_hexdigest": "15320358c81252547587e75baa27fe81", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10985.549299999984, "isoelectric_point": 9.760980796813964 }, "id": "AHCKFKLKGB_119", "locus": "GCA013367975_00079" }, { "type": "cds", "sequence": "contig_1", "start": 47064, "stop": 47222, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16AA7C", "UniRef:UniRef90_UPI001F16AA7C" ], "nt": "ATGAAGCCAATTCAATTTCAATCACTTGATAGCCGTGTATTAGCAGAACAAGTTAAATCAATAGTTGCATCAGATTATATTGATCAAACAATTACATCAAATGAAACAATCAAAAACAAATATTCAAAACGAGACAATGTAAAAGAAGTTAAGAGATAA", "aa": "MKPIQFQSLDSRVLAEQVKSIVASDYIDQTITSNETIKNKYSKRDNVKEVKR", "aa_hexdigest": "0098145e8ba22746e835912ee04130fd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 47010, "stop": 47222, "observations": { "mutation": [ 47016 ], "stop": [ 47016 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F16AA7C", "query_cov": 1.0, "subject_cov": 0.7428571428571429, "identity": 0.981, "score": 94.4, "evalue": 1.97e-24, "gene_start": 1, "gene_end": 52, "reference_start": 19, "reference_end": 70, "reference_sequence": "MDWNRKEKLEVKVTFHKPMKPIQFQSLDSRVLAEQVKSIVASDYIDQTITSNETIKNKYSKRDKVKEVKR" }, "paralog": false, "identity": 0.9571428571428572, "score": 132.0, "evalue": 5.5e-42, "description": "Internal stop codon at 47016. Nonsense mutation around 47016.", "psc": { "uniref90_id": "UniRef90_UPI001F16AA7C", "uniref50_id": "UniRef50_UPI001F16AA7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F16AA7C", "UniRef:UniRef50_UPI001F16AA7C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F16AA7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16AA7C" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6043.815600000001, "isoelectric_point": 9.454562568664553 }, "id": "AHCKFKLKGB_120", "locus": "GCA013367975_00080" }, { "type": "cds", "sequence": "contig_1", "start": 47226, "stop": 48050, "strand": "+", "frame": 3, "gene": "scpA", "product": "segregation/condensation protein A", "db_xrefs": [ "COG:COG1354", "COG:L", "KEGG:K05896", "RefSeq:WP_012855317.1", "SO:0001217", "UniParc:UPI0001BC2041", "UniRef:UniRef100_D1J7I0", "UniRef:UniRef50_N9U9Z0", "UniRef:UniRef90_D1J7I0" ], "nt": "ATGTCCGATTTAGAAAAACCAATTATTAGAGAAATTTCAATAAAAGAAGAACCAAACAAAGATCAAAACAATCAAACTAATTCATCAATTTTTAAAGAAAAACACGTATTTAAATTAAAAAATTTTGATGGTCCGCTTGATTTGTTGGTTTCTTTAATTAAAGAGAAAAATATAAGCATTTTTGATGTTGACTTATCTGAATTAGCAAATCAATATCTTGAAATAATTCATAGTCTTCAATCTTATGAAGTTGATATAGCAAGTGAATATCTAGTTATGGCTGCACATTTATTACAATTGAAAGCTAGAATGGTATTGCAAGATCCAGAAGTTGAAGAAGAAATTAGACAAGAAAAAAAGAGATTGCTCGAACAGATTGCTGAATATGAAAAATTCAAAGAAATTTCTTTACTTTTACGTGAACAAGAAGAAAAAAGATCTAGATTGTTTTCAAAAGAACCAGAAGACACACAAGATTTCGTAAGAGATATTGATGGTTCGATTCTTGACGGGCATTCCAATAGTTCAAAATTAGTTGTAACTCTAAGAAAAATGTTTGAAAGAACATATGCTGAACTTATTAGAAATATTACTATTTCAACAATAGCAGTGAGTCCCGAAGAACAAAAAGAAAGAATTATTAAATTATTTAAAACAAAGTTGCAAGTTACCTTTAAAGATGTTTTCTCTGTTCCAAGTTTGGGACACTTTGTTATTACTTTATTGGCAGCATTGGATTTAGTAAGACAACAAATTATTGAAATTTATCAAGAAGAAAATGAAGGTGAAATTCATTTTAAGAAAGGGGCTTTATATGAAGAATAA", "aa": "MSDLEKPIIREISIKEEPNKDQNNQTNSSIFKEKHVFKLKNFDGPLDLLVSLIKEKNISIFDVDLSELANQYLEIIHSLQSYEVDIASEYLVMAAHLLQLKARMVLQDPEVEEEIRQEKKRLLEQIAEYEKFKEISLLLREQEEKRSRLFSKEPEDTQDFVRDIDGSILDGHSNSSKLVVTLRKMFERTYAELIRNITISTIAVSPEEQKERIIKLFKTKLQVTFKDVFSVPSLGHFVITLLAALDLVRQQIIEIYQEENEGEIHFKKGALYEE", "aa_hexdigest": "3ad9e3d61b2247f7a3ef63311ff3136d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2041", "ncbi_nrp_id": "WP_012855317.1", "uniref100_id": "UniRef100_D1J7I0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2041", "RefSeq:WP_012855317.1", "UniRef:UniRef100_D1J7I0" ] }, "ips": { "uniref100_id": "UniRef100_D1J7I0", "uniref90_id": "UniRef90_D1J7I0" }, "psc": { "uniref90_id": "UniRef90_D1J7I0", "gene": "ScpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_N9U9Z0", "cog_id": "COG1354", "cog_category": "L", "kegg_orthology_id": "K05896" }, "pscc": { "uniref50_id": "UniRef50_N9U9Z0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U9Z0" ], "product": "Segregation and condensation protein A" }, "genes": [ "scpA" ], "id": "AHCKFKLKGB_121", "locus": "GCA013367975_00081" }, { "type": "cds", "sequence": "contig_1", "start": 48040, "stop": 48672, "strand": "+", "frame": 1, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005515", "GO:0007059", "KEGG:K06024", "RefSeq:WP_104940351.1", "SO:0001217", "UniParc:UPI000CD69D76", "UniRef:UniRef100_UPI000CD69D76", "UniRef:UniRef50_Q6KHG8", "UniRef:UniRef90_D1J7I1" ], "nt": "ATGAAGAATAAAATTATTGAAGCTTTGCTATTTATTCAAGGCTCTGAAGGGTTAAGCAGTGAACAAGCACAACAAGTTTTCAAATTAAATACAATTCAAGAAGCAAGACGATTATTAAGGGATTTTAAAACATACTTTAATAACTTGGAAAGAGGAATAAAAGTATTGGAATTCAATGATGTATTCAAGTTTTTAACTATTGAAAGCGTCAAAGATTATATTAGTGATTTAGTAACTATTACTAGAAAACAAAGATTATCAAATGCTGCAATTGAAGTTGCGGGTATTGTTGCTTATAAACAACCTATAACAAGAGGCATGATTTCAAACATAAGAGGTGTTGTAAGCGATGGAATTGTAAGTAGTTTATTAGAAAAAGGAATTATTGAAGAGGTTGGAGTTGCTCAAACTCCAGGCAACCCTATTTTATATGGAATTACTAATAAATTCTATGATTATTTCAAAATAAAGACCTTAGGGGAATTGCCTCCTTTTCCTGAATTTAATAAATATAGCAGTGAAGAAGAGGAAGAAATTGATAAAGACTTCAATTTGTTTGATTCTCAAAGACAAGAAACAGACAATGATGGAGTTTTAATTTCTAATGAAGGGGCAGAATATGATAAATTTTAA", "aa": "MKNKIIEALLFIQGSEGLSSEQAQQVFKLNTIQEARRLLRDFKTYFNNLERGIKVLEFNDVFKFLTIESVKDYISDLVTITRKQRLSNAAIEVAGIVAYKQPITRGMISNIRGVVSDGIVSSLLEKGIIEEVGVAQTPGNPILYGITNKFYDYFKIKTLGELPPFPEFNKYSSEEEEEIDKDFNLFDSQRQETDNDGVLISNEGAEYDKF", "aa_hexdigest": "e08438d076668dc77a88bafba75a0699", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000CD69D76", "ncbi_nrp_id": "WP_104940351.1", "uniref100_id": "UniRef100_UPI000CD69D76", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CD69D76", "RefSeq:WP_104940351.1", "UniRef:UniRef100_UPI000CD69D76" ] }, "ips": { "uniref100_id": "UniRef100_UPI000CD69D76", "uniref90_id": "UniRef90_D1J7I1" }, "psc": { "uniref90_id": "UniRef90_D1J7I1", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_Q6KHG8", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0007059" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHG8" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "AHCKFKLKGB_122", "locus": "GCA013367975_00082" }, { "type": "cds", "sequence": "contig_1", "start": 48644, "stop": 49492, "strand": "+", "frame": 2, "gene": "rluA", "product": "Ribosomal large subunit pseudouridine synthaseC", "db_xrefs": [ "COG:COG0564", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A448ZXK9", "UniRef:UniRef90_D1J7I2" ], "nt": "ATGAAGGGGCAGAATATGATAAATTTTAAGGCTAAAAAGGATGACGAAGGTCGTATCCTTTTTAAATATTTGGTAAAGGTTTGCGACAATGTTCCAATATCTAGAATAGAGCGACTTTTTAGAGAAAAGGATATCAAGGTAAATAATATACGTGTAAACGATAAGCAATACAAAGTCAAAGAAGGAGATTTAATTTCAATTTATGGATTAATTGAAACTTCCAAACATATTGATAGCAAAGCTAATACAAACATAAATTTTAAAGTTATTTACGAAGATAAAAATCTTTTAATTATTGACAAGGCGCCAAATATTGCAATGCATTCTGAAGAAAATTGTCTAGATGATCAAGTTTTAAAATATTTAAATTTTAAAAAAGTTTCTTCGTTTAGGCCCGCTTCAATTGGTAGAATAGATAAAAAAACTAGCGGTTTGGTTGTTTATGCAAAAAACTACAAAACTCTAGTTGAATTTGAAAACAAACAAAATAAATTTGACAAAATTTACCAATTTGTAAGTGATTTTGATGAAGATGTTTTGGATATAACAGTAAGACTAAAAAAAGATATTAAAAACGAAAAAATGATTGTCGATAATAATTTTGGCGTTAAAGCACAGACAATATTTTATAAAGAAGGAAATAGAAAATTTGCCAAGATAATAACAGGTAAAAAGCACCAAATTAGGGCGACATTAGCATATTTAAAAACACCTATTTTAGGAGATATTAAATACGGCGGAAAATTTGCTAAAAGAATGTATTTGCATTCATATTCAATAAAATTTCATGATCTTTCTGATGATTTTAAAGAATATAATGAACAAGTATTCATTTGCTATCCAAAATGA", "aa": "MKGQNMINFKAKKDDEGRILFKYLVKVCDNVPISRIERLFREKDIKVNNIRVNDKQYKVKEGDLISIYGLIETSKHIDSKANTNINFKVIYEDKNLLIIDKAPNIAMHSEENCLDDQVLKYLNFKKVSSFRPASIGRIDKKTSGLVVYAKNYKTLVEFENKQNKFDKIYQFVSDFDEDVLDITVRLKKDIKNEKMIVDNNFGVKAQTIFYKEGNRKFAKIITGKKHQIRATLAYLKTPILGDIKYGGKFAKRMYLHSYSIKFHDLSDDFKEYNEQVFICYPK", "aa_hexdigest": "3a2a67f6d181564bc75e9a6176b72886", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_D1J7I2", "query_cov": 0.9822695035460993, "subject_cov": 0.9964028776978417, "identity": 0.996, "score": 545.0, "evalue": 1.13e-195, "valid": true, "gene": "RluA", "product": "Ribosomal large subunit pseudouridine synthaseC", "uniref50_id": "UniRef50_A0A448ZXK9", "cog_id": "COG0564", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXK9" ], "product": "Ribosomal large subunit pseudouridine synthase C" }, "genes": [ "rluA" ], "id": "AHCKFKLKGB_123", "locus": "GCA013367975_00083" }, { "type": "cds", "sequence": "contig_1", "start": 49515, "stop": 49817, "strand": "+", "frame": 3, "gene": "gatC", "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C", "db_xrefs": [ "COG:COG0721", "COG:J", "RefSeq:WP_012855320.1", "SO:0001217", "UniParc:UPI0001BC2044", "UniRef:UniRef100_D1J7I3", "UniRef:UniRef50_D1J7I3", "UniRef:UniRef90_D1J7I3" ], "nt": "ATGGAAAAAGAAGAGATTAAAAAATTGGCTAATGATCTTTTATTTGAACCAAGTGAAGAAGTTATTAAATTAACTAATAACTTACTTAATTCAATAGAAAAAGGTTTAAAAGATTTAGAGGAATTTGACTTACAAGACATTAAACCGATGTCGCATATCAATGAATCACCCATTTGTTTCGAACAACTAAGAAGTGATGAACCGGATAAAAGTTTTTATTTGAATAAACAAGATTTATTAAATAATGCATCTGAACATGATGAAAATTATGTAATTATGAAGAAGGTAATAAATGATAAATAA", "aa": "MEKEEIKKLANDLLFEPSEEVIKLTNNLLNSIEKGLKDLEEFDLQDIKPMSHINESPICFEQLRSDEPDKSFYLNKQDLLNNASEHDENYVIMKKVINDK", "aa_hexdigest": "e5f45cfef76f2cb5e32c174fb203ef47", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2044", "ncbi_nrp_id": "WP_012855320.1", "uniref100_id": "UniRef100_D1J7I3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2044", "RefSeq:WP_012855320.1", "UniRef:UniRef100_D1J7I3" ] }, "ips": { "uniref100_id": "UniRef100_D1J7I3", "uniref90_id": "UniRef90_D1J7I3" }, "psc": { "uniref90_id": "UniRef90_D1J7I3", "gene": "GatC", "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C", "uniref50_id": "UniRef50_D1J7I3", "cog_id": "COG0721", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J7I3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I3" ], "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C" }, "genes": [ "gatC" ], "id": "AHCKFKLKGB_124", "locus": "GCA013367975_00084" }, { "type": "cds", "sequence": "contig_1", "start": 49807, "stop": 50709, "strand": "+", "frame": 1, "gene": "gatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "db_xrefs": [ "COG:COG0154", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A449AI30", "UniRef:UniRef90_A0A6A8PZD5" ], "nt": "ATGATAAATAAAGGTAATTTAAAAGAAGCAATTTTACAATTAAATGCTGATAAAAATAATGCGGTATCCTTTGTTTTTAACGATGCTCAATTAAAAGAACAAGGACCGCTTGCAAATTGTGTTTTTACAATTAAGGACAATTATGCAGATTTGAATGTTATTGAAAGCGCTTCAAGCAGATTTCTTTCTAATTTTAGACCTTCATATCAATCTACAATCTTAAAATTATTAAATGAAGCAGGAGCAACGTGCGTTGCAAGAACGAATTTAGATGAATTTGGTATGGGTGGCAGTGGAGAATATTCATGCAATGGATTAATAAAAAATCCTTTGAATAGTAATTATTTAGTTGGAGGTTCTTCATCAGGTGCCGCTGCAACTTTTTCTAAAAATATCTCGTTTGCAATTGGATCTGACACAGGCGATTCAGTTAGAAAACCTGCATCAAATATTGGTGTGGTTGGATTTAAACCAAGTTATGGTGCTATAAGCCGTTATGGATTGTCTGCTTTTGCTTCGTCATTAGATACAGTTGCATATTTTACACATAATGTTCAAGATGCAATAATTCTTTCATCAGTATTGTTTAAGCAAGATTTAAAACATGATTTGACATCAGTTGATTTGTCGTTTAATGCAGATAGTGTTATTGAAACAAAACCACATAAAATTGCTTATTTGGATTGTTTTGACAAATTGGATATATCTGTTGCTGATGCATATAAAAAATTATTGAATAAGCTAGAAAAAATGAATATTGAACTGGTAAAAATTTCAATAAATGAAAAGTTATTACAAGCCATCGATACTGTTTATAAGGTTATTGCTTTTTCAGAATCTTCATCTAATTTAGCAAATCTTGTAGGTGTTGAATTTGGCAATAGAATTACTGGCAATTCTTGA", "aa": "MINKGNLKEAILQLNADKNNAVSFVFNDAQLKEQGPLANCVFTIKDNYADLNVIESASSRFLSNFRPSYQSTILKLLNEAGATCVARTNLDEFGMGGSGEYSCNGLIKNPLNSNYLVGGSSSGAAATFSKNISFAIGSDTGDSVRKPASNIGVVGFKPSYGAISRYGLSAFASSLDTVAYFTHNVQDAIILSSVLFKQDLKHDLTSVDLSFNADSVIETKPHKIAYLDCFDKLDISVADAYKKLLNKLEKMNIELVKISINEKLLQAIDTVYKVIAFSESSSNLANLVGVEFGNRITGNS", "aa_hexdigest": "55a0ce3b49a87e7469114d2dfe8a6f49", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "gatA" ], "truncated": "3-prime", "pseudogene": { "start": 49807, "stop": 51012, "observations": { "mutation": [ 50707 ], "stop": [ 50707 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8PZD5", "query_cov": 1.0, "subject_cov": 0.6787330316742082, "identity": 0.99, "score": 562.0, "evalue": 9e-200, "gene_start": 1, "gene_end": 300, "reference_start": 1, "reference_end": 300, "reference_sequence": "MINKGNLKEAILQLNADKNNAVSFVFKDAQLKEQGPLANCVFTIKDNYADLNVIESASSRFLSNFRPSYQSTILKLLNEAGATCVARTNLDEFGMGGSGEYSCNGLIKNPLNSNYLVGGSSSGAAATFSKNISFAIGSDTGDSVRKPASNIGQVGFKPSYGAISRYGLSAFASSLDTVAYFTHNVQDAITLSSVLFKQDLKHDLTSVDLSFNADSVIETKPHKIAYLDCFDKLDISVADAYKKLLNKLEKMNIELVKISINEKLLQAIDTVYKVIAFSESSSNLANLVGVEFGNRITGNSWEETFNLSRTEGIGKMVQSRMILGSYFLEDNNQIKYFVRAKKMRRYLSNYFTNVHKSADIFIYPASNMSAPLIGKLYEPSYMDYILTNSNLTGNPSLTLKLGIDKNNNLPFNLAIDGEIYKDCKMLSHALFIERVLEGEKNE" }, "paralog": false, "identity": 0.9850374064837906, "score": 770.0, "evalue": 4.05e-284, "description": "Internal stop codon at 50707. Nonsense mutation around 50707.", "psc": { "uniref90_id": "UniRef90_A0A6A8PZD5", "gene": "GatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "uniref50_id": "UniRef50_A0A449AI30", "cog_id": "COG0154", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8PZD5", "UniRef:UniRef50_A0A449AI30", "COG:COG0154", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AI30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AI30" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A" } }, "id": "AHCKFKLKGB_125", "locus": "GCA013367975_00085" }, { "type": "cds", "sequence": "contig_1", "start": 50752, "stop": 51135, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "ATGGTTCAATCAAGAATGATTTTAGGATCTTATTTTTTAGAAGATAATAATCAAATAAAGTATTTTGTTAAGGCTAAAAAAATGAGAAGGTATTTAAGCAATTATTTCACTAATGTTCATAAAAGCGCAGATATATTTATCTATCCAGCATCTAACATGTCCGCTCCTCTTATTGGAAAATCATATGAACCAAGTTATATGGATTATATTTTAACAAATTCTAATTTGACGGGAAATCCTTCTTTGACATTAAAATTAGGAATTGACAAAAATAATAATCTTCCATTTAATTTGGCAATTGATGGAGAAATATATAAAGATTGCAAGATGCTAAGTCATGCATTATTTATTGAAAGAGTATTGGAAGGTGAAAAAAATGAATAA", "aa": "MVQSRMILGSYFLEDNNQIKYFVKAKKMRRYLSNYFTNVHKSADIFIYPASNMSAPLIGKSYEPSYMDYILTNSNLTGNPSLTLKLGIDKNNNLPFNLAIDGEIYKDCKMLSHALFIERVLEGEKNE", "aa_hexdigest": "8739a66543944fc254cfbeafc7d3a7ff", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 119, "aa_cov": 0.8661417322834646, "hmm_cov": 0.9915966386554622, "evalue": 1.6607451234938012e-10, "score": 35.833763122558594, "start": 2, "stop": 111 } ], "seq_stats": { "molecular_weight": 14631.690599999985, "isoelectric_point": 8.590106010437015 }, "id": "AHCKFKLKGB_126", "locus": "GCA013367975_00086" }, { "type": "cds", "sequence": "contig_1", "start": 51194, "stop": 51472, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "ATGTTTTCTCCAGTTCAAAATGAATACGGAGATGAAGCCAATACAAATGTTTCAAATATTGACCTTGCCTATCCTGGAAGTTTGCCTCTTTTAAATTCTGCTGCCATAATTAAGGCAATTAAATTAGCCAAGGCGCTAGAAATGGAAATAGATCCATTAGTTAGATTTGACAGGAAAAATTATTTTTATCCCGATTTAACAAAAGGATATCAAATTACTCAACAATTTCATCCAATTGGTAAAAATGGTTTGATAAGGGCAAAGGTTCAAAATTCATGA", "aa": "MFSPVQNEYGDEANTNVSNIDLAYPGSLPLLNSAAIIKAIKLAKALEMEIDPLVRFDRKNYFYPDLTKGYQITQQFHPIGKNGLIRAKVQNS", "aa_hexdigest": "bd4a573732d5a59ebc977f8369418c0d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 90, "aa_cov": 0.9782608695652174, "hmm_cov": 1.0, "evalue": 5.850867759791238e-33, "score": 109.510498046875, "start": 1, "stop": 90 } ], "seq_stats": { "molecular_weight": 10328.703099999992, "isoelectric_point": 7.964891624450684 }, "id": "AHCKFKLKGB_127", "locus": "GCA013367975_00087" }, { "type": "cds", "sequence": "contig_1", "start": 51500, "stop": 52549, "strand": "+", "frame": 2, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "COG:COG0064", "COG:J", "EC:6.3.5.6", "EC:6.3.5.7", "GO:0016884", "KEGG:K02434", "SO:0001217", "UniRef:UniRef50_A0A449AA70", "UniRef:UniRef90_D1J7F4" ], "nt": "ATGGAAGAAGATACTGCCAAATCAATTCACGAAAATGGTTTGACATATTTAAACTATAATAGATGTGGAGTCCCTTTAATTGAAATAGTTTCTAATCCCGTAATGCATAGTGCAGAGGATGCAATGGCTTACGTTGAAGCTATAAGACAAACAGCATTAGTTTTAGACATTAGTGATGCAAAATTAAATGAAGGAAGTTTAAGGACTGATGTTAATTTATCAGTAAGAAGAAAGGGAGAAAACATTCTTAATACTCGTACGGAAATAAAAAATTTAAATTCTCTTTCAAATATTAAAAAAGCAATTGAATATGAATCTAATTTCCAAATTCAAATGTATGAAAACAATTTAACTTTTAATCAAGAAACAAAGCGTTTTGATGAAAAAATGCAAAAGACTGTTTCAATGCGTTCGAAAGGTGATGCAATTAGTTACAAATATTTCCCAGATCCAAATTTACCTTATATTCAATTGCAACAAAAATTTATAGATGATATTAAAATAGAAGAAATACCATATCAAAAAGAAATAAGATATCTAAAAAATGGCTTAAATCAAGTTCAAATATCTCAATTATTGAATAATATTTCTTATGCAAAATATATTGATAGTATAAACTCAAAAGATTTTAAAAAAGCCACAAATGTTTTCTTTTCTGACATAGTTACCTATTTAAATAGTAACAATAAGAATATTGAAAATTTATCATTTAAAGCAGATGATGCAAGAAAGGTTGTCGAATATTTAATTGATAATAGGATTAATAAAAATAATATTGCTGAAATTATCAAAATTAAAGATCAAAATCCAAATTTAAATATTGAATCTATTATTAAAGACAATGGCTTTTTAATTGAAATAAAAGAAATTGATTTGAACGAACTAGTTCAAGAAATATTAAATGAAAATCCAAACTTGGAAACTGAATTTAAAAACAATTATAACCGTGCCCAAAAATTTATGCAAGGTCAAATAATGAAAAAAACAATGGGAAAGGCAAATCTAAAAAATCTTGATGAAATAATCAAAAATAGGTTCAATAATGAGTAA", "aa": "MEEDTAKSIHENGLTYLNYNRCGVPLIEIVSNPVMHSAEDAMAYVEAIRQTALVLDISDAKLNEGSLRTDVNLSVRRKGENILNTRTEIKNLNSLSNIKKAIEYESNFQIQMYENNLTFNQETKRFDEKMQKTVSMRSKGDAISYKYFPDPNLPYIQLQQKFIDDIKIEEIPYQKEIRYLKNGLNQVQISQLLNNISYAKYIDSINSKDFKKATNVFFSDIVTYLNSNNKNIENLSFKADDARKVVEYLIDNRINKNNIAEIIKIKDQNPNLNIESIIKDNGFLIEIKEIDLNELVQEILNENPNLETEFKNNYNRAQKFMQGQIMKKTMGKANLKNLDEIIKNRFNNE", "aa_hexdigest": "dc5ee7efd35e0af78d4134fc2d183f73", "start_type": "ATG", "rbs_motif": null, "genes": [ "gatB" ], "truncated": "5-prime", "pseudogene": { "start": 51200, "stop": 52549, "observations": { "mutation": [ 51470 ], "stop": [ 51470 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F4", "query_cov": 1.0, "subject_cov": 0.7378435517970402, "identity": 0.991, "score": 661.0, "evalue": 2.14e-237, "gene_start": 1, "gene_end": 349, "reference_start": 125, "reference_end": 473, "reference_sequence": "MNNDWEIVIGIEIHIELNTKTKMFSPIQNEYGDEANTNVSNIDLAYPGSLPLLNSSAIIKAIKLAKALEMEIDPLVRFDRKNYFYPDLTKGYQITQQFHPIGKNGLIRAKVQNSWKTFSIERIHMEEDTAKSIHENGLTYLNYNRCGVPLIEIVSNPVMHSAEDAMAYVEAIRQTALVLDISDAKLNEGSLRTDVNLSVRRKGENVLNTRTEIKNLNSLSNIKKAIEYESNFQIQMYENNLTFNQETKRFDEKMQKTVSMRSKGDAISYKYFPDPNLPYIQLQQKFIDDIKIEEIPYQKELRYLKNGLNQVQISQLLNNISYAKYIDSINSKDFKKATNVFFSDIVTYLNSNNKNIENLLFKADDARKVVEYLIDNRINKNNIAEIIKIKDQNPNLNIESIIKDNGFLIEIKEIDLNELVQEILNENPNLETEFKNNYNRAQKFMQGQIMKKTMGKANLKNLDEIIKNRFNNE" }, "paralog": false, "identity": 0.9866369710467706, "score": 876.0, "evalue": 0.0, "description": "Internal stop codon at 51470. Nonsense mutation around 51470.", "psc": { "uniref90_id": "UniRef90_D1J7F4", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.6", "6.3.5.7" ], "uniref50_id": "UniRef50_A0A449AA70", "cog_id": "COG0064", "cog_category": "J", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0016884" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F4", "EC:6.3.5.6", "EC:6.3.5.7", "UniRef:UniRef50_A0A449AA70", "COG:COG0064", "COG:J", "KEGG:K02434", "GO:0016884" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AA70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AA70" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" } }, "id": "AHCKFKLKGB_128", "locus": "GCA013367975_00088" }, { "type": "cds", "sequence": "contig_1", "start": 52824, "stop": 52979, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTATTTTTGTCTTTTATTCCAATCTCAATATTGATGCTTGGAATTATTGGCTCGGTTGATGAAAAGTATGAAGTGGTGTTAAAGAACGTAATTTTGGGTCAAATTATTCCCGGAATTTCAGAAGTAATTCCATCATTTAGTACATTATGA", "aa": "MYLFLSFIPISILMLGIIGSVDEKYEVVLKNVILGQIIPGISEVIPSFSTL", "aa_hexdigest": "69d05ef375dc0ac833d6cb7a1bce4907", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5611.733599999998, "isoelectric_point": 4.406579399108887 }, "id": "AHCKFKLKGB_129", "locus": "GCA013367975_00089" }, { "type": "cds", "sequence": "contig_1", "start": 52928, "stop": 53134, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCTTTAATTCTCGATTTTCACGCTCTGAATGGAGATTTGTGTTCATATAAAGCATCTATTGAAAAAATAAATTTTGAATATCCCTTCGATGCTAAAATTAGTACTGATAATGCAAATAAGATTAAAGCAATTGCATTGCCTGTAGAACCTCATAATGTACTAAATGATGGAATTACTTCTGAAATTCCGGGAATAATTTGA", "aa": "MNPLILDFHALNGDLCSYKASIEKINFEYPFDAKISTDNANKIKAIALPVEPHNVLNDGITSEIPGII", "aa_hexdigest": "071e74e9bbe3cabfe0c888d365e43dc1", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7468.471200000001, "isoelectric_point": 4.7775674819946286 }, "id": "AHCKFKLKGB_130", "locus": "GCA013367975_00090" }, { "type": "cds", "sequence": "contig_1", "start": 53154, "stop": 53300, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTGTCTTTAATTTTACTAGGGCTAACAGCCTTTATGGTATTTTTAATTGATAAAGTAGGGCTTGGAAAATTTGATGCCGATATTAAAAAAATGAAATTAGCAGATTTTAATTTAACTACAGAATTTTATTTAATATATTGA", "aa": "MILSLILLGLTAFMVFLIDKVGLGKFDADIKKMKLADFNLTTEFYLIY", "aa_hexdigest": "34a95c0f3e2425c725d584f3efb8db5a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5488.648999999999, "isoelectric_point": 5.955214500427247 }, "id": "AHCKFKLKGB_131", "locus": "GCA013367975_00091" }, { "type": "cds", "sequence": "contig_1", "start": 53313, "stop": 53624, "strand": "+", "frame": 3, "gene": null, "product": "YhjD/YihY/BrkB family envelope integrity protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE35A95", "UniRef:UniRef90_UPI002FE35A95" ], "nt": "TTGTTTTTACCTGTATTTACTTATTTAGGATTTCTATTTTATTTTGTATATGCCCCAAATTTTAAATTGAAAATGTCGCAAGCACATCCGGGGGCTTTGATAGCAGCAATACCAACAGCAATATTTATTTTGATTTTTGGTTCATTAACATCCTTAATTCCTTATAAAAAATTTGGGATAGTTGCTTCATTTATGTATATTATTTTATTATTGAGTTTTACTTCTTATTTTATTTATATGGGAATTATTGTTAATTCAAGTTTTTATAAAACTTTTATTAATTTACCAACTGTAGAAAAACATTCATTTTGA", "aa": "MFLPVFTYLGFLFYFVYAPNFKLKMSQAHPGALIAAIPTAIFILIFGSLTSLIPYKKFGIVASFMYIILLLSFTSYFIYMGIIVNSSFYKTFINLPTVEKHSF", "aa_hexdigest": "f4d5323e4ec5f05e3006e13736be685c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 53015, "stop": 53704, "observations": { "insertion": [ 53063, 53619, 53632 ], "stop": [ 53109, 53298 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE35A95", "query_cov": 1.0, "subject_cov": 0.42738589211618255, "identity": 0.99, "score": 194.0, "evalue": 7.69e-61, "gene_start": 1, "gene_end": 103, "reference_start": 113, "reference_end": 215, "reference_sequence": "MRFYRQCNCFNLICAIGINFSIEGIFKIYFSIDALYEHKSPFRAWKSRIKGFIVSVFVCLILSLILLGLTAFMVFLIDKVGLGKFDADIKKMKLADFNLTTEFYLIYWFTTILFLPVFTYLGFLFYFVYAPNFKLKMSQAHPGALIAAIPTAIFILIFGSLTSLIPYKKFGIVASFMYIILLLSFTSYFIYMGIIVNSSFYKTFINLPTVEKHSFWKNKNFNLKSFDFFANFSLFIVINDI" }, "paralog": false, "identity": 0.9692982456140351, "score": 414.0, "evalue": 5.51e-150, "description": "Internal stop codon at 53109, 53298. Frameshift due to insertion around 53063, 53619, 53632.", "psc": { "uniref90_id": "UniRef90_UPI002FE35A95", "product": "YhjD/YihY/BrkB family envelope integrity protein", "uniref50_id": "UniRef50_UPI002FE35A95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE35A95", "UniRef:UniRef50_UPI002FE35A95" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE35A95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE35A95" ], "product": "YhjD/YihY/BrkB family envelope integrity protein" } }, "id": "AHCKFKLKGB_132", "locus": "GCA013367975_00092" }, { "type": "cds", "sequence": "contig_1", "start": 53844, "stop": 54593, "strand": "+", "frame": 3, "gene": null, "product": "ECF transporter S component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I4", "UniRef:UniRef90_D1J7I4" ], "nt": "GTGGGTGTTTTGATTGCTATTTCAGTTGTCTTTTTTCTAATAAGTGTTAGAATCATGCCCATAAGTGCCTTACCAAGTATAAAATTTAGCTTTATTGGTTTGCCAATAAAAATAACTGGTTTTATTTTTGGACCAATTGTTGGTATGATAACCGGGATATTGGCGGATATAATTTCATTTGCTCTAGTTCCAACTTACTATAATGTTTTGTATACAATAGCGGTTGCGGTATCAGGGTTTGTTCCCGGGTTTGCTGCGTACTATTTTTTCAATATTAATGAATTGTTTTTTTCAAGAACGTATAGAATTTTTAAATACAAAGAAACAATTGATTATTTTAAAGAACAATATGATATTGCACTTACAATAGGTAACTCCGAAGATATGCAATATTTTTCAGAAAAAATTGCTTTTTATGAAGTCAAGATCATAATTTTAGAATGTAAGAAAAAACCAATTGCCATGATTAATTTTTCATTTATTTCAACATTGATAATATTAGGTATTCAGGTGTTGATAATTCTTGCAATTTTTTCTAACCTAGATCCTTCAATATTTGAAAAAAATAGATTTGTTAAAAACAAAAAATTCTATATGTTCTTAACTTCTATAGGATTTATAGGAATGATGATTTTTGTTTCTCTATATAGAATATTTTTAAAGAAAAAATTTGAAACCTTTTTAGAAATAATGGCAATAATTTCATTTAGCGCTGTTTTAGAAATAGTAAATGTTATTTTATTGGCGTGA", "aa": "MGVLIAISVVFFLISVRIMPISALPSIKFSFIGLPIKITGFIFGPIVGMITGILADIISFALVPTYYNVLYTIAVAVSGFVPGFAAYYFFNINELFFSRTYRIFKYKETIDYFKEQYDIALTIGNSEDMQYFSEKIAFYEVKIIILECKKKPIAMINFSFISTLIILGIQVLIILAIFSNLDPSIFEKNRFVKNKKFYMFLTSIGFIGMMIFVSLYRIFLKKKFETFLEIMAIISFSAVLEIVNVILLA", "aa_hexdigest": "9db49f7cfba2e9009cbeac8f6e97cb26", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 53745, "stop": 54749, "observations": { "mutation": [ 53820, 54591 ], "stop": [ 53820, 54591 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I4", "query_cov": 0.9959839357429718, "subject_cov": 0.7425149700598802, "identity": 0.992, "score": 430.0, "evalue": 3.97e-150, "gene_start": 2, "gene_end": 249, "reference_start": 35, "reference_end": 282, "reference_sequence": "MNKLPGGLRHYIYWARSQTNISYRKWNSKRIAFVGVLIAISVVFFLISVRIMPISALPSIKFSFIGLPIKITGFIFGPIVGMITGILADIISFALVPTYYNVLYTIAVAVSGFVPGFAAYYFFNINELFFSRTYRIFKYKETIDYFKEQYDIALTIGNSEDMQYFSEKIAFYEVKIIILECKKKPIAMINFSFISTLIILGIQVLIILAIFSNLDPSIFEKNRFVKNKKFYMFLTSIGFIGMMIFVSLYRIFLKKKFETFLEIMAIISFSAVLEIVNVILLAWADTSTLKTDFWVNFTAQSLFSPVKIFFNLAIILATYKVVAPLIKSKEGGHL" }, "paralog": false, "identity": 0.9940119760479041, "score": 630.0, "evalue": 1.82e-231, "description": "Internal stop codon at 53820, 54591. Nonsense mutation around 53820, 54591.", "psc": { "uniref90_id": "UniRef90_D1J7I4", "product": "ECF transporter S component", "uniref50_id": "UniRef50_D1J7I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I4", "UniRef:UniRef50_D1J7I4" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I4" ], "product": "ECF transporter S component" } }, "id": "AHCKFKLKGB_133", "locus": "GCA013367975_00093" }, { "type": "cds", "sequence": "contig_1", "start": 54749, "stop": 55159, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAGCAATAAGTTCTTTAAAAGTTACCAATTTAAAAAGTATTAATGTTGTAAATGAACAATTTAAAAACTTAATTAATAATCTATCTTTTACACTTAATGCAAACAATATATTGGGTTTTGTAGGTGGAGATAGTGAAACAAATAATTTAATATTTAATTATTTAACTAATAATACAATAAATCCTAAAAGATATCAAGGTTACATTGAATTTCAAACAAACTTAGGTGAATATTCTTCAATATATAAGAACTCAAATTATCAAAATAACATAGCTTTTGGTTTTTATAATGATATTTTAAACAATGATTTAAATGAAGATAATAGTTATAGAAATATAAAAAAATATATTAACCACGGAGCAGTTATAGGGGCACTAACGGAAGAATTTGTTTCTCAGTGA", "aa": "MKKIAISSLKVTNLKSINVVNEQFKNLINNLSFTLNANNILGFVGGDSETNNLIFNYLTNNTINPKRYQGYIEFQTNLGEYSSIYKNSNYQNNIAFGFYNDILNNDLNEDNSYRNIKKYINHGAVIGALTEEFVSQ", "aa_hexdigest": "7074d2209fe7d3c109b94223dda4f3ea", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15508.084699999981, "isoelectric_point": 6.56259593963623 }, "id": "AHCKFKLKGB_134", "locus": "GCA013367975_00094" }, { "type": "cds", "sequence": "contig_1", "start": 55187, "stop": 56104, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E424311", "UniRef:UniRef90_UPI001E424311" ], "nt": "TTGCTTCAAAGCGAATTTGCAAAGTTACAATATGGTGTTGAAATTGAACTTAAAAGCAAATTAGACGATTTATTCAACGAATTAAATCAGTTTGATTTGCGAAATATCGACGCTAAAAATTTAAAATTTGATACTTTTTTTAATAAATTTAAAGATATAGAAATTTTATATAGAACCCATGAAATGAAACTATTCGCATTTGTTAATGCAACTATAGCAAAATATAATAAGGGGTTATCGTTTTTAAAACGCAAGGAATATTTAGACAGTAAAAAGGAATACGAAGAGGTAATAAAAAAAATTTATAAGCCTTTTTCAAAGCCTAATAGATTTTTAAATCGAGTAAGATTTTTTTCTAAACTTTTAGAAACAAAAAAATCTTCAAAATCATCTATTAAAAAATTTGTAAATAAATTAAGGCGTGATTTAAATATTGAAAACTATTATTACAAATATGAATTAAAGAAAACAATAAAACAATCTTCGTTTATTCACTTTTATAAAAATCTATATTTTAATGGTCTATTATTGAAATTTATAAATAAAAATTTAAAGGCCATATTAGGGCTAAAAGAAGCAAAATCGTTTGAATTGTTGTATGAAATTTTTGATTTAAAAAACAATATTACAAATGAAGTTAATGTAATTTCAAACATTGATAATCAAAAAGAATTAAAGAATGAAATAAAGGCTGTAATAAAAAATAATTTTTTAAATGATGTGATGCCATATATTGAAAGAATTAAGGCGTCTAGTTATGAACACACTCAAGAAGTTATTAATGATGTTACAAGAGAAATTCAAACGTCTAATAACTACGATTTAAACATAGGCGAGTTTGGCCTTGCAAACAAAGCGTTTGAAAAATTAAAAACAGTTGAATCAAAATATAAAGATGCAAAAAATGAATATTTTTGA", "aa": "MLQSEFAKLQYGVEIELKSKLDDLFNELNQFDLRNIDAKNLKFDTFFNKFKDIEILYRTHEMKLFAFVNATIAKYNKGLSFLKRKEYLDSKKEYEEVIKKIYKPFSKPNRFLNRVRFFSKLLETKKSSKSSIKKFVNKLRRDLNIENYYYKYELKKTIKQSSFIHFYKNLYFNGLLLKFINKNLKAILGLKEAKSFELLYEIFDLKNNITNEVNVISNIDNQKELKNEIKAVIKNNFLNDVMPYIERIKASSYEHTQEVINDVTREIQTSNNYDLNIGEFGLANKAFEKLKTVESKYKDAKNEYF", "aa_hexdigest": "0e91fa40d657d624e2d8031a0ec815ff", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 54887, "stop": 56149, "observations": { "mutation": [ 55157, 56102 ], "stop": [ 55157, 56102 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E424311", "query_cov": 1.0, "subject_cov": 0.6475583864118896, "identity": 0.9740000000000001, "score": 562.0, "evalue": 3.09e-199, "gene_start": 1, "gene_end": 305, "reference_start": 147, "reference_end": 451, "reference_sequence": "MKKIAISSLKVTNLKSINVVNEQFKNLINNLSFTLNANNILGFVGGDSETNNLIFNYLTNNTINPKRYQGYIEFQTNLGEYSSIYKNSNYQNNIAFGFYNDILNNDLNEDNSYRNIKKYINHGAVIGALTEEFVSQWKPVFQDYKLLLQSEFAKLQYGVEIELGSKLDDLFSELSQFDLRNIDAKNLKFDTLFKKFKDIEILYRTHEMKLFAFVNATIAKYNKGLSFLKRKEYLDSKKEYEEVIKKIYKPFSKPNRFLNRVRFFSKFLETKKSSKSSIKKFVNKLRRDLNIENYYYKYELKKTIKQSSFIHFYKNLYFNGLLLKFINKNLKAILGLKEAKSFELLYEIFDLKNNITNEVNVISNIDNQKELKNEIKAVIKNNFLNDVMPYIERIKASSYEHTQEVINDVTREIQTSNNYDLNIGEFGLANKAFEKLKIVESKYKDAKNEYFWNYKTEGNAILKETKSFKKF" }, "paralog": false, "identity": 0.9785714285714285, "score": 815.0, "evalue": 2.24e-301, "description": "Internal stop codon at 55157, 56102. Nonsense mutation around 55157, 56102.", "psc": { "uniref90_id": "UniRef90_UPI001E424311", "uniref50_id": "UniRef50_UPI001E424311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E424311", "UniRef:UniRef50_UPI001E424311" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E424311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E424311" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 36488.65950000005, "isoelectric_point": 9.476159477233889 }, "id": "AHCKFKLKGB_135", "locus": "GCA013367975_00095" }, { "type": "cds", "sequence": "contig_1", "start": 56225, "stop": 56884, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATTTAATAAAAATTAAGGAATTTAATAATTCAGTTCTAAATGAGCAAGTAAAAAAAATAAAAGAAAATCTACAATTTTTAAAAATTTTAAGAAATGAATTTGATTCTTATAAATATGTTGTAAGTGATTTTAAAACGGCATCAAAAAAACAAATAAAACTTTTTGTTTCAAAAATTAATATTTACAAAATTATTGCAAAGGCAAATATTGACGTTAATTTTTTAGTAGAGAAATTAGGCTCTTTAAGCGATGATGAAAAAATTAGATTAGAGTGCGAAAAAATTTTAATAAATAATCCTTCGCTAATAATAATAGGACCCAATATTTCTAAATTACCATATGAAAAGCAATGCGAAACATTGAATAAATTGAACAAATTCGTTTTAGACAATCACAAAATGGCAATTTATTTCTTAGACAATATAAATATTGCTCAAAAAATAACTACAGATTTATATATTATAAATTCATCAAAAATTATTGAGCAAGGAAAAACTAGTCAATTAATAAGAAACCCAATAAATCCTATAGTCAAAAATATGTTAAATTATAAAGATGAGAAGACTCAAGAGGATTTAAGCTTCTATATGTCTCAAGAAGACAATATGGAAAACATATACAAATATGAAATAGAGCCATACCACTTTGTTTGA", "aa": "MINLIKIKEFNNSVLNEQVKKIKENLQFLKILRNEFDSYKYVVSDFKTASKKQIKLFVSKINIYKIIAKANIDVNFLVEKLGSLSDDEKIRLECEKILINNPSLIIIGPNISKLPYEKQCETLNKLNKFVLDNHKMAIYFLDNINIAQKITTDLYIINSSKIIEQGKTSQLIRNPINPIVKNMLNYKDEKTQEDLSFYMSQEDNMENIYKYEIEPYHFV", "aa_hexdigest": "e760e6a53e0320f0234620b36f3627cf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25769.726700000025, "isoelectric_point": 8.778933906555174 }, "id": "AHCKFKLKGB_136", "locus": "GCA013367975_00096" }, { "type": "cds", "sequence": "contig_1", "start": 56945, "stop": 57130, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTATTTATTCCACAAAATACCTTTCAAAGTAAAGGAAATAAAACAATAGATAATAAGGAACCATTCGAAGAAACAACGCTTATAAATTTAATCGATGACGAAGATAACGAACTAAGGAATTTGAACATGGAAAAAACTTTTAATCATAAAATAGTTGAAAAAGACAGATGCAAAAAATGA", "aa": "MSLFIPQNTFQSKGNKTIDNKEPFEETTLINLIDDEDNELRNLNMEKTFNHKIVEKDRCKK", "aa_hexdigest": "d00d099dfced2fa91f162c6214778f5a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7257.111500000001, "isoelectric_point": 5.284571266174315 }, "id": "AHCKFKLKGB_137", "locus": "GCA013367975_00097" }, { "type": "cds", "sequence": "contig_1", "start": 57137, "stop": 57298, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGAAGATTTTTCAAACTCACGATGAATCTAAAAAACCATTCACCATTATTTTGCCACCCCCAAATGTAACCGGCAATCTCCATATTGGCCATGCCCTAGACAATTATATTTCTGATACAATAATAAGGTTTAAGAAGTTACAGGGATATGATGTATTGTGA", "aa": "MKIFQTHDESKKPFTIILPPPNVTGNLHIGHALDNYISDTIIRFKKLQGYDVL", "aa_hexdigest": "a7f9fee12811d4164b5043ad99892ddd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 47, "aa_cov": 0.8867924528301887, "hmm_cov": 1.0, "evalue": 1.2846885146874334e-17, "score": 58.95497131347656, "start": 6, "stop": 52 } ], "seq_stats": { "molecular_weight": 6078.990000000001, "isoelectric_point": 8.123548316955567 }, "id": "AHCKFKLKGB_138", "locus": "GCA013367975_00098" }, { "type": "cds", "sequence": "contig_1", "start": 57470, "stop": 57595, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGCCCTTGATTATGGTTTTGAAAGATTCACATTAGATGATAATTCTAATGAAGCTGTATTAAAGGTATTTATTGAACTTTATGAAAATGGTTTAATATATCGTGACAAAAAAGCAATAAATTGA", "aa": "MALDYGFERFTLDDNSNEAVLKVFIELYENGLIYRDKKAIN", "aa_hexdigest": "abc8b3314988d02db92af6055c58a28c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 39, "aa_cov": 0.9512195121951219, "hmm_cov": 1.0, "evalue": 1.482481682002237e-09, "score": 32.398719787597656, "start": 3, "stop": 41 } ], "seq_stats": { "molecular_weight": 4829.396900000001, "isoelectric_point": 4.507070732116699 }, "id": "AHCKFKLKGB_139", "locus": "GCA013367975_00099" }, { "type": "cds", "sequence": "contig_1", "start": 57659, "stop": 58141, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "ATGTACTACATAAAATATCCAATTAAAAGTTCTGAAAGCTTTCTTGAAGTAGCCACAACGAGACCCGAAACAATGTTTTCAGATGTAGCTTTAGCAATAAACCCACAAGATAGTAGAGCAAAACAATTAATAAAAGAAGTAATAATACATCCATTGACAAACAAAGAAATTCCAATTATTTTATCAAACAAAATAGATCCTAATTTTGGTAGTGGAATAATGAAAGTTTCAGCCCATGCTGTTGATGATATTGAAATTATTATTGAAAATAAACTCCCAATAAATGAATGTATCAATAAAGATGGGAAATTAAACGATTTAGCAGTTGAATTTAAGGGATTGGATCGCTTTGAAGCTAGAGAAAAAATTGCACACTTATTAAGTCAAAAAGGACTTTTAATAAAAGAAGAAGAAGTAGTTTCTAATATTGGATATAGTGAAAGATCAAAGGCTCCAATTGAAATTTTAGTTCAGCCACAATGA", "aa": "MYYIKYPIKSSESFLEVATTRPETMFSDVALAINPQDSRAKQLIKEVIIHPLTNKEIPIILSNKIDPNFGSGIMKVSAHAVDDIEIIIENKLPINECINKDGKLNDLAVEFKGLDRFEAREKIAHLLSQKGLLIKEEEVVSNIGYSERSKAPIEILVQPQ", "aa_hexdigest": "d1e43956634fef4d42ee0cc2764a0fa3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 61, "aa_cov": 0.375, "hmm_cov": 1.0, "evalue": 4.361553438778463e-11, "score": 37.92393112182617, "start": 47, "stop": 106 } ], "seq_stats": { "molecular_weight": 18014.650899999986, "isoelectric_point": 5.462306785583496 }, "id": "AHCKFKLKGB_140", "locus": "GCA013367975_00100" }, { "type": "cds", "sequence": "contig_1", "start": 58523, "stop": 58777, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTATTTCTTTAGTTTATATTTTATGGATCAAAAACCTTTTGAACATCTTTTATTGCATGGATTAGTTAGAGATTCACAAGGAAGAAAAATGTCTAAATCGCTTGGCAATGGAATTAATCCAATTCAAGTAATTGATGAATACGGTTCTGATGTTTTAAGAATGTCTTTAATTTTTAATTGCACACCTGGCCTTGATATTAATTACAGTGATGAAAAAATCCAAGGTGCTAAACTATTTGCTAATAAATTCTGA", "aa": "MYFFSLYFMDQKPFEHLLLHGLVRDSQGRKMSKSLGNGINPIQVIDEYGSDVLRMSLIFNCTPGLDINYSDEKIQGAKLFANKF", "aa_hexdigest": "2c3b7d7103de409c769653338b70449a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001D01CCB6", "query_cov": 0.9642857142857143, "subject_cov": 0.9310344827586207, "identity": 0.58, "score": 106.0, "evalue": 1.57e-28, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PEY5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 69, "aa_cov": 0.8214285714285714, "hmm_cov": 0.9855072463768116, "evalue": 2.758768819047994e-22, "score": 74.33128356933594, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 9653.03739999999, "isoelectric_point": 6.705830192565918 }, "id": "AHCKFKLKGB_141", "locus": "GCA013367975_00101" }, { "type": "cds", "sequence": "contig_1", "start": 59063, "stop": 59596, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGTTTCTAACAGATTATGTTTTTGAAAAATTGTATCATGAAGAATTGCTATGCAGCAATCTTCAAACTTTTGCCTTAAATAAAGAAAGTTCAAACAATACTCCTAAAATTATTTCTTTAATTACTGCTTTAAGAAAATATAGGGAAGATAAAAAGATTTCAAAATCTATTGAATTATTCTTCTATTTGAATAATCTTGTATTCAGCAGTATAGATTTAAAAATAATTAACAAATTAAGTAATTTTTCTTGGAAAGAAAATCATGACTTTTCACTAAAAACAGAAAGTTTTGAAGTATTTATAATTCAAAGTAAGCAAGACAAGGAAAATGAAATTCTAGAATTAAATAAATTAATTGAATTTAGTCAAAAAGAAATTGAATTTAATGAAAAATTAATTAATAATCCTAAATTTATGGAAAAGGCTAACAAAAATACAATCAATGAAAAATTAGCAAACTTAAAATTGCATAAAGAAAAATTAGAGTTTTATAAAAACCAACTTAAAGAAAAACAAGAAAAAATAAATTAA", "aa": "MPFLTDYVFEKLYHEELLCSNLQTFALNKESSNNTPKIISLITALRKYREDKKISKSIELFFYLNNLVFSSIDLKIINKLSNFSWKENHDFSLKTESFEVFIIQSKQDKENEILELNKLIEFSQKEIEFNEKLINNPKFMEKANKNTINEKLANLKLHKEKLEFYKNQLKEKQEKIN", "aa_hexdigest": "40054b217056c135a015a07379ba39ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21209.17510000002, "isoelectric_point": 8.428999519348146 }, "id": "AHCKFKLKGB_142", "locus": "GCA013367975_00102" }, { "type": "cds", "sequence": "contig_1", "start": 59917, "stop": 60345, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGAATAATGAAGTTGAACACCTTCAAAAATTATTAGTAGGATTTAAACGTCCTTTTGTTGCAATAATTGGTGGGGCAAAAGTAAGCGATAAAATCAAAGTTCTAGAAAAGTTATTTGAACTAGCAGATAAGGTTTTAATTGTTGGTGGTATGGCATATACATTCCAAAAAGCCTTAAATAGAAAAATTGGAACTTCTATTTTTGAAGAAGATAAAGTTGAAATGGCAAAAGAATATTTAAACAAATATAAAGATAAAATTGTTTTGCCAATTGACAATGCTTATGTCGATGAATTTGCTGACAAAACTCCTAAATTTACAAGTCCAGAAAATCCTGATATTCCTGACGGAAATATGGGATTAGATATTGGACCAGAAAGTATTAAATTATTTGCAAGCATTATTAAAGATGCTAAAACTATCTTTTGA", "aa": "MNNEVEHLQKLLVGFKRPFVAIIGGAKVSDKIKVLEKLFELADKVLIVGGMAYTFQKALNRKIGTSIFEEDKVEMAKEYLNKYKDKIVLPIDNAYVDEFADKTPKFTSPENPDIPDGNMGLDIGPESIKLFASIIKDAKTIF", "aa_hexdigest": "62d79ad13d7edce897ac9733b4b7d5dc", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 145, "aa_cov": 1.0, "hmm_cov": 0.993103448275862, "evalue": 1.2144572003841326e-45, "score": 151.40011596679688, "start": 1, "stop": 142 } ], "seq_stats": { "molecular_weight": 15924.39339999999, "isoelectric_point": 5.9026384353637695 }, "id": "AHCKFKLKGB_143", "locus": "GCA013367975_00103" }, { "type": "cds", "sequence": "contig_1", "start": 60457, "stop": 60870, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGAAGAAAACTATTGAAGACCTTCAATTGGAAAACAAAAAGGTTATTTTAAGAGTTGATTTTAACGTTCCCATTTCAAACAACACCATTATGGACACAAAAAGAATTGATGAAGAATTGCCAACAATCAAATATATATTGAGTAAAAATGCTTCTTTGATCATTCTATCTCATTTAGGAAGAGTTAAAACAGAAGAAGACAAAAAGACTAAATCTTTAGAAATTGTAGCTAAAAAATTAGCGCAATTACTAAATAGAGATGTTAAATTTATTAACGAAAATAGAAGTGAAGCAGTTACAAATGCTGCTAAAAACTTAAAACCTGGAGAAATTTTGGTTTTGGAAAATACTCGTTTTCAAGATTTAAATAATAAAGCAGAATCTAATAATGATGAAGAACTTGCAAAATATTGA", "aa": "MKKTIEDLQLENKKVILRVDFNVPISNNTIMDTKRIDEELPTIKYILSKNASLIILSHLGRVKTEEDKKTKSLEIVAKKLAQLLNRDVKFINENRSEAVTNAAKNLKPGEILVLENTRFQDLNNKAESNNDEELAKY", "aa_hexdigest": "f3577e9c2dd39a4d024f5587867052be", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 133, "aa_cov": 0.9635036496350365, "hmm_cov": 0.9548872180451128, "evalue": 1.2189111384216683e-40, "score": 134.93797302246094, "start": 5, "stop": 136 } ], "seq_stats": { "molecular_weight": 15720.913899999981, "isoelectric_point": 8.729293251037596 }, "id": "AHCKFKLKGB_144", "locus": "GCA013367975_00104" }, { "type": "cds", "sequence": "contig_1", "start": 60982, "stop": 61158, "strand": "+", "frame": 1, "gene": null, "product": "YARHG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYF4", "UniRef:UniRef90_D1J7I7" ], "nt": "ATGGCAAAAATTTTAAATGCTCAAAAGCAAAAGGACACAAGAACTCTTAGCTATGATCCTGAAAAAGACAGCTTGACTTTGCTAATTTCAGAGTTTCAACTTTACAATAAAAACACCAAATTTATTAATGATTATTCAGAAATGGAAAAATTCCACTATTTTAAAGAATTTAGATGA", "aa": "MAKILNAQKQKDTRTLSYDPEKDSLTLLISEFQLYNKNTKFINDYSEMEKFHYFKEFR", "aa_hexdigest": "ea50b9f6f9f9d9aca0b4de39d3389e5f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 60982, "stop": 61389, "observations": { "mutation": [ 61156, 61261, 61306 ], "stop": [ 61156, 61261, 61306 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I7", "query_cov": 1.0, "subject_cov": 0.4264705882352941, "identity": 0.983, "score": 111.0, "evalue": 3.26e-30, "gene_start": 1, "gene_end": 58, "reference_start": 1, "reference_end": 58, "reference_sequence": "MAKILNAQKQKDTRTLSYDPEKDSLTLLISEFQLYNKNTKFINDYSEMEKFHYFKESRWMKTMDEKLQDQVQEFSKRQKKLNAKYKSYIEYSKWSESPYATKTKPLTKWNRIIIISVFIILVIIMLLIVIGLNKLW" }, "paralog": false, "identity": 0.9703703703703703, "score": 237.0, "evalue": 2.46e-82, "description": "Internal stop codon at 61156, 61261, 61306. Nonsense mutation around 61156, 61261, 61306.", "psc": { "uniref90_id": "UniRef90_D1J7I7", "uniref50_id": "UniRef50_A0A448ZYF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I7", "UniRef:UniRef50_A0A448ZYF4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYF4" ], "product": "YARHG domain-containing protein" } }, "id": "AHCKFKLKGB_145", "locus": "GCA013367975_00105" }, { "type": "cds", "sequence": "contig_1", "start": 61647, "stop": 61991, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAAGAACTGATGGTGTAATATTAAATGCTAACATTTCTGCTTCTCTAATTATTTCTATTTTTAATAAACACGCTCCACTTCATGATGGCGCAATAATTATTAGAAATAACAAAATTTATTATGCTGCCACATATTATAAAATAACAAAAAAATCAATTGATAATAAATATGGCGCAAGACACCGTGCTGCTATGGGTATTTCAGAAATTTGCGATGCTACAACTATAGTTGTTAGTGAAGAAAATGGTGGAATTACAATAGCTAAAAACGGAACTTTTAGCCCTGTTTTGCTAGAAAATTTACAAGAAAGATTAGTCAATATTTTCAAGGATAATAATTAA", "aa": "MLRTDGVILNANISASLIISIFNKHAPLHDGAIIIRNNKIYYAATYYKITKKSIDNKYGARHRAAMGISEICDATTIVVSEENGGITIAKNGTFSPVLLENLQERLVNIFKDNN", "aa_hexdigest": "e9d95699979e2d59e9bd131c9670276f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12594.339599999987, "isoelectric_point": 9.098052406311037 }, "id": "AHCKFKLKGB_146", "locus": "GCA013367975_00106" }, { "type": "cds", "sequence": "contig_1", "start": 62564, "stop": 63757, "strand": "+", "frame": 2, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "COG:COG0050", "COG:J", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "RefSeq:WP_012855329.1", "SO:0001217", "UniParc:UPI0000129DB6", "UniRef:UniRef100_P22679", "UniRef:UniRef50_Q8Y422", "UniRef:UniRef90_P22679" ], "nt": "ATGGCAAAATTAGATTTTGACCGTTCAAAACCACACGTTAATATTGGTACTATCGGTCACGTTGACCACGGTAAAACTACATTAACTGCTGCTATTGCAACAGTATTAGCTAAAAAAGGTCTAGCAGAAGCTAGAGACTACGCTTCTATTGACAATGCACCAGAAGAAAAAGCACGTGGTATTACAATTAACACATCACACATTGAATATCAAACAGAAAAACGTCACTACGCACACGTTGACTGTCCAGGACACGCCGACTATGTTAAAAACATGATTACCGGTGCTGCTCAAATGGATGGAGCAATTTTAGTTGTTGCTGCTACTGATGGTCCTATGCCTCAAACTCGTGAACACATTTTACTTGCAAGACAAGTTGGTGTTCCAAAGATCGTTGTATTTTTAAACAAAATTGATATGTTTAAAGATGACGAAAGAGAAGAAATGGTTGGACTAGTAGAAATGGACGTAAGAAGCCTTCTATCAGAATATGGTTTTGATGGCGACAATGCTCCTATTATTGCTGGTTCAGCTCTTAAAGCATTACAAGGAGATCCAGAATACGAAAAAGGTATTTTGGAATTAATGGATGCTGTTGATACATACATCGAAGAACCAAAACGTGAAACAGACAAACCATTCTTAATGGCTGTAGAAGACGTATTTACAATTACTGGTCGTGGAACTGTTGCAACTGGTAGAGTTGAACGTGGTGTTTTACAATTAAACGAAGAAGTTGAAATTGTTGGATTGAAACCAACCAAGAAAACAGTTGTTACTGGTATTGAAATGTTCCGTAAGAACTTAAAAGAAGCACAAGCTGGAGATAACGCTGGATTATTGCTACGTGGAATTGACAGAAGCGAAGTTGAACGTGGACAAGTTTTAGCAAAACCTAAGACAATCGTTCCTCACACACAATTCGAAGCAACTGTTTATGTTCTTAAAAAAGAAGAAGGTGGACGTCACACACCATTCTTCCACAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACTGGTGGAATTGAATTCAAACCTGGACGTGAAATGGTTGTTCCTGGCGACAACGTAGAATTAACTGTTACTTTAATTGCTCCTATTGCTATTGAAGAAGGAACCAAGTTCTCTATTCGTGAAGGTGGAAGAACTGTTGGTGCCGGATCAGTTACAAAAATTCTTAAATAA", "aa": "MAKLDFDRSKPHVNIGTIGHVDHGKTTLTAAIATVLAKKGLAEARDYASIDNAPEEKARGITINTSHIEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVPKIVVFLNKIDMFKDDEREEMVGLVEMDVRSLLSEYGFDGDNAPIIAGSALKALQGDPEYEKGILELMDAVDTYIEEPKRETDKPFLMAVEDVFTITGRGTVATGRVERGVLQLNEEVEIVGLKPTKKTVVTGIEMFRKNLKEAQAGDNAGLLLRGIDRSEVERGQVLAKPKTIVPHTQFEATVYVLKKEEGGRHTPFFHNYKPQFYFRTTDVTGGIEFKPGREMVVPGDNVELTVTLIAPIAIEEGTKFSIREGGRTVGAGSVTKILK", "aa_hexdigest": "094091eaca3165ab26beac6d706d2680", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000129DB6", "ncbi_nrp_id": "WP_012855329.1", "uniref100_id": "UniRef100_P22679", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129DB6", "RefSeq:WP_012855329.1", "UniRef:UniRef100_P22679" ] }, "ips": { "uniref100_id": "UniRef100_P22679", "uniref90_id": "UniRef90_P22679" }, "psc": { "uniref90_id": "UniRef90_P22679", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_Q8Y422", "cog_id": "COG0050", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q8Y422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8Y422" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "AHCKFKLKGB_147", "locus": "GCA013367975_00107" }, { "type": "cds", "sequence": "contig_1", "start": 64200, "stop": 65144, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_277872611.1", "SO:0001217", "UniParc:UPI002410D0FC", "UniRef:UniRef100_UPI002410D0FC", "UniRef:UniRef50_UPI001F51FD84", "UniRef:UniRef90_UPI001F51FD84" ], "nt": "ATGATAAAGAAAAGAAGAAGAAAATTAGGATGCTTGACCTTTATTGCCGGAGGAGTTGCTGTTGCTGCTTTCGTTCTAATAATGTGTAGATTTTGCAAGAAACCTAATAAACAAATAAAAGATCGAGCCTCTTTAAATTTATTTAATAAGGCACAAAGTGAATTAAAAGAATTAATAAATTCTCCAATAGCTAAGCTATGTCAAACTGCTATTGAACAAAAAGTTTTAAACGATTCAAATAAAATAAATGAAAAGTCTACTGACGAAGAAATTTCAAAGAATACAGAATATTTAAATAGTTCAATCGCTTTGCTTAAAAATAAGTTAGATCTTGCAAAACAAGAATTAGAAAAATTCAACATGGCTATGTTTCAACTAAGATCATTAGTTAATAATAAAGATAGTAAAAGTATAGATACTCAAAATTCAATCAATGCTTTAAATAATGCTAAAAAATTGCCTCTTAATTCTATTGAACAAGTTAAGAGCGAATCTTTAACTTTGAAAAATAATATTGATGAATTAAAGTTAAAGCTTAAAGAAGTTAAAAAGTCTAGACTTGCAGAAGCCAAGAACAAATTAAAAGCAATTATTGATTCAGAATCTTCTAAATATGTTGATAGTTCTAGAGAATTGCAAATATTTAATGATTCAAATGTTGAAGAAAAATCAACACTTGAAGATATTGATAAAAAAATTGATGCTGCAATAAGAGCAAACAATGATTTAAATGAAAAAATTCAAAATTACAAATCAGCAAGATTATCTGATCTTGAATCTTCAAGAAATGATTTGTCAAACTTTATTGCAAACGATTTAAAAGATCCTGTATATAAAGAAATTGCAAATAAAATTAGTGAAAAGATTAAAGAAGTAAAAGGCGTTGGAAAAAATTCAACAATCAAAAGAATTGCAAGCAGCCATAAAAACTTTAGCAGATGCTAA", "aa": "MIKKRRRKLGCLTFIAGGVAVAAFVLIMCRFCKKPNKQIKDRASLNLFNKAQSELKELINSPIAKLCQTAIEQKVLNDSNKINEKSTDEEISKNTEYLNSSIALLKNKLDLAKQELEKFNMAMFQLRSLVNNKDSKSIDTQNSINALNNAKKLPLNSIEQVKSESLTLKNNIDELKLKLKEVKKSRLAEAKNKLKAIIDSESSKYVDSSRELQIFNDSNVEEKSTLEDIDKKIDAAIRANNDLNEKIQNYKSARLSDLESSRNDLSNFIANDLKDPVYKEIANKISEKIKEVKGVGKNSTIKRIASSHKNFSRC", "aa_hexdigest": "c28932b3da6ac033c34d8e190dc67dfc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI002410D0FC", "ncbi_nrp_id": "WP_277872611.1", "uniref100_id": "UniRef100_UPI002410D0FC", "db_xrefs": [ "SO:0001217", "UniParc:UPI002410D0FC", "RefSeq:WP_277872611.1", "UniRef:UniRef100_UPI002410D0FC" ] }, "ips": { "uniref100_id": "UniRef100_UPI002410D0FC", "uniref90_id": "UniRef90_UPI001F51FD84" }, "psc": { "uniref90_id": "UniRef90_UPI001F51FD84", "uniref50_id": "UniRef50_UPI001F51FD84" }, "pscc": { "uniref50_id": "UniRef50_UPI001F51FD84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51FD84" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35527.486000000055, "isoelectric_point": 9.537855720520017 }, "id": "AHCKFKLKGB_148", "locus": "GCA013367975_00108" }, { "type": "cds", "sequence": "contig_1", "start": 65083, "stop": 65409, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAAATTCAACAATCAAAAGAATTGCAAGCAGCCATAAAAACTTTAGCAGATGCTAAAGAAAAAGCAATTAAAGAAAAAGCTTGGATAGATAGAGTTGGTATTAAAAAAGTTTTAGAAATTCAAAGATCTAAAATATTGTTAGTTGATGCTGAATATATTGCAAGATTTTTAAGATCAGGAAATGGTGAACGTTTTAGACAAGAGAATGAATTTAATAAATATTCCGGAACAGAATTTTTAAAATTAAAAAATGAGTTGGAAAGGGTTTCAAATCTTAAAGATTTAAATGCTCTTGACAGTGTTGATTATAAAGAAATATGA", "aa": "MEKIQQSKELQAAIKTLADAKEKAIKEKAWIDRVGIKKVLEIQRSKILLVDAEYIARFLRSGNGERFRQENEFNKYSGTEFLKLKNELERVSNLKDLNALDSVDYKEI", "aa_hexdigest": "6086dd1c0376d74bfac347038509a877", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12588.330899999986, "isoelectric_point": 9.096827507019043 }, "id": "AHCKFKLKGB_149", "locus": "GCA013367975_00109" }, { "type": "cds", "sequence": "contig_1", "start": 65803, "stop": 65940, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002735871D", "UniRef:UniRef90_UPI001F1864BA" ], "nt": "TTGAACAATAGCAAATTAAATAAACAAGAAATTAAGAATTTTAAAGAAAAATTAATTGCAAAAAATCTAGAAATTGAAGAATATTTAAGTAGCGAAGAAACCTTTAAAGATTATCGTATATATAACCCTAGTCTATGA", "aa": "MNNSKLNKQEIKNFKEKLIAKNLEIEEYLSSEETFKDYRIYNPSL", "aa_hexdigest": "9b1f78f0f94744b38287475023e804e2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 65803, "stop": 66099, "observations": { "mutation": [ 65938, 65956 ], "stop": [ 65938, 65956 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F1864BA", "query_cov": 1.0, "subject_cov": 0.45918367346938777, "identity": 0.867, "score": 75.1, "evalue": 1.29e-16, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MNNSKLDKQEIKDFKEKLSAKNLEIEKYLSSEETFKDYRAYNPDLWMNNLSYDIKEQEKILNDINSKISLIEENSFTNQELKNTFAELKQKLSILNKN" }, "paralog": false, "identity": 0.8061224489795918, "score": 153.0, "evalue": 5.16e-50, "description": "Internal stop codon at 65938, 65956. Nonsense mutation around 65938, 65956.", "psc": { "uniref90_id": "UniRef90_UPI001F1864BA", "uniref50_id": "UniRef50_UPI002735871D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F1864BA", "UniRef:UniRef50_UPI002735871D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002735871D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002735871D" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5454.1251, "isoelectric_point": 6.188140678405762 }, "id": "AHCKFKLKGB_150", "locus": "GCA013367975_00110" }, { "type": "cds", "sequence": "contig_1", "start": 66432, "stop": 67019, "strand": "-", "frame": 2, "gene": "tdk", "product": "thymidine kinase", "db_xrefs": [ "COG:COG1435", "COG:F", "EC:2.7.1.21", "GO:0004797", "GO:0005524", "RefSeq:WP_073673795.1", "SO:0001217", "UniParc:UPI00093D3F1C", "UniRef:UniRef100_UPI00093D3F1C", "UniRef:UniRef50_Q5ZZT0", "UniRef:UniRef90_D1J7J2" ], "nt": "ATGTATAAAACATTTAATGATGGAATGATAGAAGTTATCACTGGACCAATGTTTAGTGGAAAAAGTGAAGAATTATTAAGGAGAGTTAGAACCTTAGAATATGCTAAGATGCTACCGCTTTTAATAAAGCCCGAATATGATAATCGCTTTTCTGATGATGAAATAGTAAGCCGTTGTGGCGTAAGAAGAAAAACGCATTCATTAAAAAACATCAATGAAGTTTATGAATTACTTAAGCAAGATAAATATAAGGCAATAATAATTGACGAAGCCCATTTTTTTGGGAATTCATTAATTGAAGTCGCTGATGACTTAGCAAATAAGGGATATTTAGTAATTGTAGCTGGCCTAGACCAAGATTATCTAAGAAAACCTTTTGGGCCAATTCCTGCCTTAATGGCAATGGCTGAACGAGTAACTAAATTACAAGCAATTTGCGTAGTATGTCAACATGCTGCTTCAACGAGTTTTAGAAAAGATTCTTCAAATGAACAAAACTTAATTGGTGACTATAATGAATATGAAGCAAGATGCAGAAAGTGCCACATAATAGGTCAAAAACAAAAGGAATTACTAAAAAATAAGTAA", "aa": "MYKTFNDGMIEVITGPMFSGKSEELLRRVRTLEYAKMLPLLIKPEYDNRFSDDEIVSRCGVRRKTHSLKNINEVYELLKQDKYKAIIIDEAHFFGNSLIEVADDLANKGYLVIVAGLDQDYLRKPFGPIPALMAMAERVTKLQAICVVCQHAASTSFRKDSSNEQNLIGDYNEYEARCRKCHIIGQKQKELLKNK", "aa_hexdigest": "47fc38de18b54b1b70db1b6c9c8cb3d3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00093D3F1C", "ncbi_nrp_id": "WP_073673795.1", "uniref100_id": "UniRef100_UPI00093D3F1C", "db_xrefs": [ "SO:0001217", "UniParc:UPI00093D3F1C", "RefSeq:WP_073673795.1", "UniRef:UniRef100_UPI00093D3F1C" ] }, "ips": { "uniref100_id": "UniRef100_UPI00093D3F1C", "uniref90_id": "UniRef90_D1J7J2" }, "psc": { "uniref90_id": "UniRef90_D1J7J2", "gene": "Tdk", "product": "thymidine kinase", "ec_ids": [ "2.7.1.21" ], "uniref50_id": "UniRef50_Q5ZZT0", "cog_id": "COG1435", "cog_category": "F", "go_ids": [ "GO:0004797", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_Q5ZZT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5ZZT0" ], "product": "Thymidine kinase" }, "genes": [ "tdk" ], "id": "AHCKFKLKGB_151", "locus": "GCA013367975_00111" }, { "type": "cds", "sequence": "contig_1", "start": 67094, "stop": 67984, "strand": "+", "frame": 2, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "COG:COG0012", "COG:J", "GO:0005524", "GO:0005525", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47270", "UniRef:UniRef90_D1J7J3" ], "nt": "ATGAGTTTAAAAGCTGGTATAGTTGGATTGCCTAATGTTGGCAAAAGTACATTGTTTTCTGCATTAACATTAAATGAAGCTGAAATGGCAAATTATGCTTTTACTACAATTGAGCCTAACGTTGCAATAGTTAATTTAGAAGATCCAAGGCTTTATGAATTAGCTAAAATAGTTCAAACAGAAAAAATTGTTCCTGCTACTTTTCAATTTGTTGATATAGCTGGATTAGTTGCTGGTGCTTCAAAAGGAGAAGGCTTAGGCAATAAGTTTTTAGCAAATATTCGCGAAGTTGATGCAATTGTTCATGTTATAAGATGCTTCGAAAATGCTGATATAATGCATGTTAATAATAAAATTGATCCTATTAGTGATTTAAAAACTATTAATATTGAATTAATATTGGCAGATTTGCAAACTATTGAAAATGTAATTAATAGAATTGGCAAAAAAGCTCAAAATTCAGGTGATAAAGAATTGAAATTCGAATTTGAAGTTGCCTTAAAAATACGTAATGCATTAGAAAATGAAATTATGGTTAAAAATATGGATTTAAATCCAGAAGAACAAGCCATGATCAAATCATATCAATTGCTTACTGCAAAACCTACAATCTATGTTGCAAACCTTGATAGTGATAGTTTTAAAAATCTAGAAAGTGCTAAATATTATCAAGAATTAAACAAATATTTGCAAGACAAAAAAGAAATTTTAATACCTATTTGTATTAAATTAGAATATGAATTATGTAAATTTAATGATGAAGAAAAAGAAATGTTTTTAAATGAATATAACATTGAATGCTCCGGATTAAATTTAATTATCAAGAAAAGTTTTTATTTATTAAATCAAGCAACATATTTTACGGCAGGCAAAATTGAAGCAAGGGCATGA", "aa": "MSLKAGIVGLPNVGKSTLFSALTLNEAEMANYAFTTIEPNVAIVNLEDPRLYELAKIVQTEKIVPATFQFVDIAGLVAGASKGEGLGNKFLANIREVDAIVHVIRCFENADIMHVNNKIDPISDLKTINIELILADLQTIENVINRIGKKAQNSGDKELKFEFEVALKIRNALENEIMVKNMDLNPEEQAMIKSYQLLTAKPTIYVANLDSDSFKNLESAKYYQELNKYLQDKKEILIPICIKLEYELCKFNDEEKEMFLNEYNIECSGLNLIIKKSFYLLNQATYFTAGKIEARA", "aa_hexdigest": "340f68d6d9412b3abfa69e1a8c584927", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J7J3", "query_cov": 1.0, "subject_cov": 0.8065395095367848, "identity": 0.986, "score": 563.0, "evalue": 2.53e-201, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P47270", "cog_id": "COG0012", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47270" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "AHCKFKLKGB_152", "locus": "GCA013367975_00112" }, { "type": "cds", "sequence": "contig_1", "start": 68000, "stop": 68197, "strand": "+", "frame": 2, "gene": "ychF", "product": "YchF C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A382QXD8" ], "nt": "ATGAATGCTGCTCAATGTGCAGGAATTATTCACACTGATTTTGAAAAGAAATTTGTAAAAGCAGAAGTCATTAAATATGAAGATTATGTTGCCTTAAATGGCGAAAAAGGTTGCAGGGAAGCTGGAAAAATAGCAATTGAGGGCAAGAATTATATTATGCAAGATGGTGATGTTTGCTTTTTTAGAGTGGCAAAATAA", "aa": "MNAAQCAGIIHTDFEKKFVKAEVIKYEDYVALNGEKGCREAGKIAIEGKNYIMQDGDVCFFRVAK", "aa_hexdigest": "204250049ecabfea5f2f80e5dd4fa1f8", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A380DK80", "query_cov": 0.9538461538461539, "subject_cov": 0.8051948051948052, "identity": 0.629, "score": 89.7, "evalue": 2.79e-22, "valid": false, "product": "GTP-binding and nucleic acid-binding protein YchF", "uniref50_id": "UniRef50_A0A382QXD8" }, "pscc": { "uniref50_id": "UniRef50_A0A382QXD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A382QXD8" ], "product": "YchF C-terminal domain-containing protein" }, "genes": [ "ychF" ], "id": "AHCKFKLKGB_153", "locus": "GCA013367975_00113" }, { "type": "cds", "sequence": "contig_1", "start": 68521, "stop": 69090, "strand": "+", "frame": 1, "gene": null, "product": "Polymerase/histidinol phosphatase N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L9U4", "UniRef:UniRef90_D1J7J4" ], "nt": "ATGAATTTAATAAAAATTGATTTGCATGTACATTCTCAGGATTCAAGCCATACAGGAAGTAATATTTCCCAAGAATCGGATTTAGAAAAATTGTTAATTTTAACTAAAAAAAGAGTCAAAATTGCTAGTTTTTCTGACCATGATAATTTTTATATTGAAAGTTATTTGAAACGCCTTGAAATAATCAAACAATACAATATTGATATTACATTATGGCCTGGTATTGAAGTTAATTTAAGAAAATATGATGGTCAAATAGGACAAGCTATATTTATATTTGATCCAGATTGCAACTTAAAAGAATTAAATGAAGTAACTAAAAAGGAATTTAGATTCAATAGCAATAAATACACATACAAAGACGCTGTTGAACTATTTAAAAAAAGCAACTTCAATTTTATGGTTTTCCCTCATGCTGGAAAAGGTCAAGATAATATGGAATGGCAAGATATATGCAATAGCCAAGTTGATGGGCTGGATATAACAGATTTTAATAACAGCAACAAAAAGAAAATATTAAAACAAGATCCAAACATTCCAGTTTTATATTTTAGTGACACACATACATGA", "aa": "MNLIKIDLHVHSQDSSHTGSNISQESDLEKLLILTKKRVKIASFSDHDNFYIESYLKRLEIIKQYNIDITLWPGIEVNLRKYDGQIGQAIFIFDPDCNLKELNEVTKKEFRFNSNKYTYKDAVELFKKSNFNFMVFPHAGKGQDNMEWQDICNSQVDGLDITDFNNSNKKKILKQDPNIPVLYFSDTHT", "aa_hexdigest": "fe71eba9cf2b66da9412a281b2a8d16d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_D1J7J4", "query_cov": 1.0, "subject_cov": 0.8217391304347826, "identity": 0.995, "score": 382.0, "evalue": 9.59e-134, "valid": true, "product": "Polymerase/histidinol phosphatase N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A077L9U4" }, "pscc": { "uniref50_id": "UniRef50_A0A077L9U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L9U4" ], "product": "Polymerase/histidinol phosphatase N-terminal domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_154", "locus": "GCA013367975_00114" }, { "type": "cds", "sequence": "contig_1", "start": 69225, "stop": 69392, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13476.12" ], "nt": "ATGATAAAAAGCCTAAAAATTAAAAATAATAACGAAACTTATGACATAAATTTAAATAATCAAGTTAATGTTATTGTTGGTGAAAAGGGTTCTGGTAAAAGCACCTTGCTTTTGATTTTAGCAGAAGCAATAGTAAACAAAAAATTATTCAAAAACGAATATGAATGA", "aa": "MIKSLKIKNNNETYDINLNNQVNVIVGEKGSGKSTLLLILAEAIVNKKLFKNEYE", "aa_hexdigest": "6a444988ddbae595e6e1b6cf9a716b5e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13476.12", "name": "AAA_23", "length": 28, "aa_cov": 0.509090909090909, "hmm_cov": 0.9285714285714286, "evalue": 8.341726453035547e-08, "score": 28.387985229492188, "start": 13, "stop": 40 } ], "seq_stats": { "molecular_weight": 6208.142099999999, "isoelectric_point": 9.050087928771973 }, "id": "AHCKFKLKGB_155", "locus": "GCA013367975_00115" }, { "type": "cds", "sequence": "contig_1", "start": 69663, "stop": 70256, "strand": "+", "frame": 3, "gene": null, "product": "AAA family ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491A3C", "UniRef:UniRef90_UPI001E491A3C" ], "nt": "ATGTTAAAACAATTAGATGATTTTAAATTGCTAGAAGATTTGTTAAATGAATATAGGCGCGAACGCGATAGACAGATAGCACTTTCAAATGTTTTTAATCAATCTTTTAATTTAAATGAAGGTAGTACTAAAAACATTGCAACCATTGACTATGATTCTTCAAATGAAATAAAAAAAATTAAGGAATTATTGAAATCAATTGAAGAAACAAGAGAAAAAATAAATAGTACAAATTTGGTTGTCAATAATCTTTTGGCACAATTTGAACAATCTTCGTCGCTACTTCAAATTTCAAATGAACAATTATTGGATATTCAACAAAGAACAGAAAATGTTATACTACAAAATAACTCATTTCTAGAATATTACAATCAATATAATTTAGTTGTATCCAAAGATAAAAAAACTCTTGAATGTTTTAAATTATCATTTACAAATGCTAAAAGAGCATATCTTGAAGAATTAAGCAACACTTTGAAGATTCAAAATGATAATGAACAATTATTGGAACATTTTTCTAATTTAGGAAGAATAATGAAAAAAATCAATAGACAATATAATTCAATAATTGAAAAAGAAATCATTTTAAACTGA", "aa": "MLKQLDDFKLLEDLLNEYRRERDRQIALSNVFNQSFNLNEGSTKNIATIDYDSSNEIKKIKELLKSIEETREKINSTNLVVNNLLAQFEQSSSLLQISNEQLLDIQQRTENVILQNNSFLEYYNQYNLVVSKDKKTLECFKLSFTNAKRAYLEELSNTLKIQNDNEQLLEHFSNLGRIMKKINRQYNSIIEKEIILN", "aa_hexdigest": "6324355ed785b857d0239e217855ee95", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 69363, "stop": 70283, "observations": { "mutation": [ 69390, 70254 ], "stop": [ 69390, 70254 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E491A3C", "query_cov": 1.0, "subject_cov": 0.5549295774647888, "identity": 0.975, "score": 354.0, "evalue": 9.24e-121, "gene_start": 1, "gene_end": 197, "reference_start": 147, "reference_end": 343, "reference_sequence": "MIKSLKIKNNNETYDINLNSQVNVIVGEKGSGKSTLLLILAEAIVNKKLFKNEYEWFNEKAKFLVDSINIDNTEIKTQDFSYIFDSENKQNSKDAGILEIQKNLSGYISQNDSRKNSLDSSEYVEKQKSNCIDNFTEKIYYDQDQKMLKQLDDFRLLEDLLNEYRRERDRQIALSNVFNQSFNLNEGSTKNIATIDYDSSNEIKKIKELLKSIEETKEKIYSTNLVVNNLLAQFEQSSSLLQISNEQLLDIQQRTENVILQNNLFLEYYNQYNLVVSKDKKALECFKLSFTNAKRAYLEELSNTLKIQNDNEQLLEHFSNLGRIMKKINRQYNSIIEKEIILNWTLEETDKKIRI" }, "paralog": false, "identity": 0.9771241830065359, "score": 580.0, "evalue": 4.64e-212, "description": "Internal stop codon at 69390, 70254. Nonsense mutation around 69390, 70254.", "psc": { "uniref90_id": "UniRef90_UPI001E491A3C", "product": "AAA family ATPase", "uniref50_id": "UniRef50_UPI001E491A3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E491A3C", "UniRef:UniRef50_UPI001E491A3C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E491A3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491A3C" ], "product": "AAA family ATPase" } }, "id": "AHCKFKLKGB_156", "locus": "GCA013367975_00116" }, { "type": "cds", "sequence": "contig_1", "start": 70434, "stop": 70904, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E54A28D", "UniRef:UniRef90_UPI001E54A28D" ], "nt": "ATGATTAAGGCATTGATTAAAGATAAAATAAAAATCTATGCAGGGGATGAAGAATATAAAAATCTATCAATGGGCCAAAAGACATTGTTTGGTATAACACACGCGATTGATACATTGAACAATGTTCCTAATGAACAATACTTATTGCTAGACCAAATTGAAGATAATCTAGACAATAAAACAATATATGAAAAAATAGTTCCAATGTTGCAAGAACAAATTAAAAAAGACAAACAAATTTTTATAGTTACACATAACCCTAATATTGGAACATTAATAAAAGGCAATATAATAACAACAGATATTTTCAATACTAATCTAAACAAAAAATTTGTTGTTAATGAAATAATTCGCGAAGATAATGAAATTGACACACCTCAATCGCTTTATTTGGAGGGCAGTATGAAAGCCTTATATGAAAGATTAAAAGTACATGAAGAAAAAATTAATAAAACTAAAGGAGATAAATAA", "aa": "MIKALIKDKIKIYAGDEEYKNLSMGQKTLFGITHAIDTLNNVPNEQYLLLDQIEDNLDNKTIYEKIVPMLQEQIKKDKQIFIVTHNPNIGTLIKGNIITTDIFNTNLNKKFVVNEIIREDNEIDTPQSLYLEGSMKALYERLKVHEEKINKTKGDK", "aa_hexdigest": "61a3ccd52d71209f6c79ef85da4fbc0c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E54A28D", "query_cov": 1.0, "subject_cov": 0.8387096774193549, "identity": 0.9940000000000001, "score": 301.0, "evalue": 7.72e-103, "valid": true, "product": "ATP-binding protein", "uniref50_id": "UniRef50_UPI001E54A28D" }, "pscc": { "uniref50_id": "UniRef50_UPI001E54A28D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E54A28D" ], "product": "ATP-binding protein" }, "genes": [], "id": "AHCKFKLKGB_157", "locus": "GCA013367975_00117" }, { "type": "cds", "sequence": "contig_1", "start": 70904, "stop": 71026, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7J6", "UniRef:UniRef90_D1J7J6" ], "nt": "ATGATAACAATTGACTATGTATTTACAAAAGATGAAAAGAGATTAATAGTTATTTCTAATGCAAGCGATAGCAAAAATAAATATAAAATTGAAATTGACTTAGACAACCCTTCTGATGCTTGA", "aa": "MITIDYVFTKDEKRLIVISNASDSKNKYKIEIDLDNPSDA", "aa_hexdigest": "0b5c43c96b7a5c397768c5edef3831e1", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 70904, "stop": 71200, "observations": { "mutation": [ 71024 ], "stop": [ 71024 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7J6", "query_cov": 1.0, "subject_cov": 0.40816326530612246, "identity": 1.0, "score": 78.2, "evalue": 6.29e-18, "gene_start": 1, "gene_end": 40, "reference_start": 1, "reference_end": 40, "reference_sequence": "MITIDYVFTKDEKRLIVISNASDSKNKYKIEIDLDNPSDAWNKENINNFIIRAISISDEKLSEPQLTESAQEQLQKGNKQIEFIKNLFTNFVERYNEN" }, "paralog": false, "identity": 0.9795918367346939, "score": 187.0, "evalue": 1.03e-63, "description": "Internal stop codon at 71024. Nonsense mutation around 71024.", "psc": { "uniref90_id": "UniRef90_D1J7J6", "uniref50_id": "UniRef50_D1J7J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7J6", "UniRef:UniRef50_D1J7J6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7J6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4604.149700000001, "isoelectric_point": 4.663718986511231 }, "id": "AHCKFKLKGB_158", "locus": "GCA013367975_00118" }, { "type": "cds", "sequence": "contig_1", "start": 71308, "stop": 71832, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGAAGAATGGTAAAGAAGTTAAAATGTCAAAACGTTTAGGAACCTCTTTAACGTTAAGAGAACTAGCAGAAGAAGTTGGAAATGATGCAATTAGATACTTTCTAATTGATAGATCATATAATTCAAGAATTGACTTTGATATTAACAAAGTTACTGACAACAACGAAAATAACCCTATGTATATAATTAAATATGCTCATGCTCGTGCTTGCCAATTATTGGATAAAGTAGGAATTAAAAACCCTATTGCTTCTGATGAGTTGGATAACGAATATGCTCAAAAGCTTGTTGCAGAATTAAAAGAATACCCTGAATTAATTTCAACAATGGCCAAAACTTATAAAGTAAACTTGTTGCCTCCTTATCTATTAAAATTATCAAAAGCATTTAACTCATTTTATTCAAATACCAAAGTTTTAGGATCTCCTGACGAACAAAGCCTTGCAGCCCTAGTAAAGGCAACAAAAATTGTTTTAGCTGATGGTTTAAAATTAATGGACATTGAAGCACCTGAAAAAATGTAA", "aa": "MKNGKEVKMSKRLGTSLTLRELAEEVGNDAIRYFLIDRSYNSRIDFDINKVTDNNENNPMYIIKYAHARACQLLDKVGIKNPIASDELDNEYAQKLVAELKEYPELISTMAKTYKVNLLPPYLLKLSKAFNSFYSNTKVLGSPDEQSLAALVKATKIVLADGLKLMDIEAPEKM", "aa_hexdigest": "3d3740b113071afe38003fc3e5d0d38a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 117, "aa_cov": 0.6436781609195402, "hmm_cov": 1.0, "evalue": 1.5006199817761587e-29, "score": 97.8059310913086, "start": 63, "stop": 174 } ], "seq_stats": { "molecular_weight": 19667.520299999996, "isoelectric_point": 7.708924674987793 }, "id": "AHCKFKLKGB_159", "locus": "GCA013367975_00119" }, { "type": "cds", "sequence": "contig_1", "start": 72139, "stop": 72573, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTGCTAACCCAACAGGATATTTGCATATTGGACACGCAAGAAATGCTGCTATTGGTGCAACATTGTGCAATATTCTAGAAAAAGCAGGTCACCGCGTTGTTCGAGAATATTTAGTTAATGATTATGGAAACCAAATGAATAAAATGGCAGATTCAATATTTGCTAGATACCAACAAATATTTAATCCCAAATTTCCTATGCCAGAAGATTCATACCATGGCGGAGATATGATAGAATTCGCTCAAGCATTTTATGACATTCACAAAGATGAATACAAAAATGTAGAATATACTGATGAAATTAGAAAAATCTTTAGAAGTTTTGGAAGAGATTTTGCTTTAAAAAATATTATTGAAGATATGAAAAAATTTGGAGTAACATTCGATATTTTCACATCAGAAGCAGAACAATATGCCAAAAATAGAGTATGA", "aa": "MSANPTGYLHIGHARNAAIGATLCNILEKAGHRVVREYLVNDYGNQMNKMADSIFARYQQIFNPKFPMPEDSYHGGDMIEFAQAFYDIHKDEYKNVEYTDEIRKIFRSFGRDFALKNIIEDMKKFGVTFDIFTSEAEQYAKNRV", "aa_hexdigest": "1acaa45099a82b9ee23a8d2571667471", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16721.70229999999, "isoelectric_point": 6.19598445892334 }, "id": "AHCKFKLKGB_160", "locus": "GCA013367975_00120" }, { "type": "cds", "sequence": "contig_1", "start": 72679, "stop": 72933, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03485.21" ], "nt": "ATGAAAACAACGAAAGACATTATTATTGCTTCATTAAATGAAGCACTTGAAAAATTAAATATTTCTAAGCCTATTGTACTTACTGAATCTAAAAATTACGGCGACTATTCAAGCAATATTGCCTTAACATTGCAAAAGGATTTAGGTAAGAAGGCAATAGATATTGCCCAAGAAATAGTAAGAAATATTGATTTAAATAAATTTAAAGAAATTAGTGAAATAAAGGTTGCCGAACCTGGTTTTATTAATTTTTGA", "aa": "MKTTKDIIIASLNEALEKLNISKPIVLTESKNYGDYSSNIALTLQKDLGKKAIDIAQEIVRNIDLNKFKEISEIKVAEPGFINF", "aa_hexdigest": "c4fb2651a79feec6cd8afa5b3577dae8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03485.21", "name": "Arg_tRNA_synt_N", "length": 81, "aa_cov": 0.9404761904761905, "hmm_cov": 0.9753086419753086, "evalue": 7.818388644722985e-20, "score": 66.67340087890625, "start": 6, "stop": 84 } ], "seq_stats": { "molecular_weight": 9409.805399999996, "isoelectric_point": 6.232872962951659 }, "id": "AHCKFKLKGB_161", "locus": "GCA013367975_00121" }, { "type": "cds", "sequence": "contig_1", "start": 72994, "stop": 73476, "strand": "+", "frame": 1, "gene": null, "product": "Cysteine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "nt": "ATGAAAAGATATTATTTATGTGGCCCAACTGTTTATAATTATCCCCATATAGGCAATTTAAGGCCAATAGTTAGTTTTGATTTAATGATTAGGGCTCAAAGGCAATTGGGAGAAGATGTTTATTTTCTTCACAATATCACTGATATAGATGACAAAATCATTGCTAAGGCAAAACAAGAAAACAAGACAGAAAAAGAAATTTCAAGTTTTTATGAAGCATATTATTTAAATTTATTTAAATTATTCTCAATTGAAATGCCAAATAAGATTGTAAGAGTTACTGATTCCCTTAATGATATGTATGAATATATTCAATCATTGATTGAAAAGGGCGCAGCATATCAAGTGAACGGCAATGTCTATTTTGACGTTTTGAAATTTAAAGATGTGTATGGAAAAATAAGCAATCAAAAATTGGCAAATTTAATTTCAGATTCAAGCAATGAAGTTGACAAGCGCTCACCATATGATTTTGCTTTATGA", "aa": "MKRYYLCGPTVYNYPHIGNLRPIVSFDLMIRAQRQLGEDVYFLHNITDIDDKIIAKAKQENKTEKEISSFYEAYYLNLFKLFSIEMPNKIVRVTDSLNDMYEYIQSLIEKGAAYQVNGNVYFDVLKFKDVYGKISNQKLANLISDSSNEVDKRSPYDFAL", "aa_hexdigest": "53e44d9bdfe39edeeac9ba27ba052aca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZZN9", "query_cov": 0.8, "subject_cov": 0.9624060150375939, "identity": 0.672, "score": 190.0, "evalue": 1.14e-59, "valid": false, "product": "Cysteine--tRNA ligase", "uniref50_id": "UniRef50_A0A448ZZN9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "product": "Cysteine--tRNA ligase" }, "genes": [], "id": "AHCKFKLKGB_162", "locus": "GCA013367975_00122" }, { "type": "cds", "sequence": "contig_1", "start": 73702, "stop": 74121, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF01406.26" ], "nt": "TTGAATTATAAGAATCAAAAGATGTCTAAGTCAATAGGAAACATAATTTATGCTGAAGAATTTTTAAAAAATTATTCAGCTGATACTTATAAATTGTTAATGCTATCAACAAGTTACAGCAAACCAATTAATATTACTGATGAATTACTTGCATCGCTTCAAAAACAAATTAATAATATTGAGGTGCATTTTAATAAAAGTCAATTGGAAGATATTAAATATACGCTAAACAAACAAACAATAGATTCAATTTTAAAAAATGTTTCTAATCTTGATTTTTCATTAGCCTTTAAGCAACTTAATGAGCAATTAAAATCACCATTGAATTATGGTAATGCGTTTTTTGTATTTAAGGTTTTAGGCTTTAAATTAGTTGAAAATAAAGTAAGCAATGATGATAAAGAACTATATAGAAAGTGA", "aa": "MNYKNQKMSKSIGNIIYAEEFLKNYSADTYKLLMLSTSYSKPINITDELLASLQKQINNIEVHFNKSQLEDIKYTLNKQTIDSILKNVSNLDFSLAFKQLNEQLKSPLNYGNAFFVFKVLGFKLVENKVSNDDKELYRK", "aa_hexdigest": "e47b30531437d02f81802d5f6a4409a6", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 42, "aa_cov": 0.302158273381295, "hmm_cov": 1.0, "evalue": 1.1182414472177045e-07, "score": 26.214223861694336, "start": 4, "stop": 45 }, { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 51, "aa_cov": 0.3669064748201439, "hmm_cov": 1.0, "evalue": 2.630176979446446e-10, "score": 35.412010192871094, "start": 2, "stop": 52 } ], "seq_stats": { "molecular_weight": 16177.372499999989, "isoelectric_point": 9.116554832458494 }, "id": "AHCKFKLKGB_163", "locus": "GCA013367975_00123" }, { "type": "cds", "sequence": "contig_1", "start": 74199, "stop": 74666, "strand": "+", "frame": 3, "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "db_xrefs": [ "COG:COG0566", "COG:J", "GO:0006396", "GO:0008173", "SO:0001217", "UniRef:UniRef50_C4XFX7", "UniRef:UniRef90_D1J7J9" ], "nt": "ATGAAAAAGATAGTTTTTGGAAAAAATTCAGTCTTAGAAGCAATTGAATATGAATTTCCTATAGAAGAGATTTACTTGCAAAAAGGACTTGTTAACAATAATTTAAAATTTAAGAAAGTTGTTTATTTAGATAGAAATAGCCTAGATAAATTAACAGAAAATGGAAATCACCAAGGATATGCTGCAAAATTAAAAGATTTTCAATATCATGATTTGGGTAGCATTTTTAAAGATGGCTCAGATAAGGTTTTAATTTTAGACCATATCCAAGACCCCCAAAATTTTGGTGCTATTATCCGTAGTGCCAATGTGTTTGGCTTTAAACATATAATCATTCCAAAGGATAGAGCATGTGATGTAACTCCGAGTGTTTTAAAAACTAGCTCAGGAGGTTTTAAAAACGTTAAAGTAATTAAAGTTAACAGCTTATTTGATGCAATTGAGGAATTAAAAAATAAAGGTTTTTGA", "aa": "MKKIVFGKNSVLEAIEYEFPIEEIYLQKGLVNNNLKFKKVVYLDRNSLDKLTENGNHQGYAAKLKDFQYHDLGSIFKDGSDKVLILDHIQDPQNFGAIIRSANVFGFKHIIIPKDRACDVTPSVLKTSSGGFKNVKVIKVNSLFDAIEELKNKGF", "aa_hexdigest": "1e54981a63b0844ac6a0f58473b6586c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rlmB" ], "truncated": "3-prime", "pseudogene": { "start": 74199, "stop": 74891, "observations": { "mutation": [ 74664 ], "stop": [ 74664 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7J9", "query_cov": 1.0, "subject_cov": 0.6739130434782609, "identity": 1.0, "score": 313.0, "evalue": 6.4e-107, "gene_start": 1, "gene_end": 155, "reference_start": 1, "reference_end": 155, "reference_sequence": "MKKIVFGKNSVLEAIEYEFPIEEIYLQKGLVNNNLKFKKVVYLDRNSLDKLTENGNHQGYAAKLKDFQYHDLGSIFKDGSDKVLILDHIQDPQNFGAIIRSANVFGFKHIIIPKDRACDVTPSVLKTSSGGFKNVKVIKVNSLFDAIEELKNKGFWIYASALNQQAKKLNDAKFNEKIAIIVGNEGSGVSKTLLKHSDEIIYIEQDKESVQSLNVSVATGILLYTVFNKK" }, "paralog": false, "identity": 0.9956521739130435, "score": 449.0, "evalue": 5.42e-163, "description": "Internal stop codon at 74664. Nonsense mutation around 74664.", "psc": { "uniref90_id": "UniRef90_D1J7J9", "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "uniref50_id": "UniRef50_C4XFX7", "cog_id": "COG0566", "cog_category": "J", "go_ids": [ "GO:0006396", "GO:0008173" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7J9", "UniRef:UniRef50_C4XFX7", "COG:COG0566", "COG:J", "GO:0006396", "GO:0008173" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFX7" ], "product": "RNA 2-O ribose methyltransferase substrate binding domain-containing protein" } }, "id": "AHCKFKLKGB_164", "locus": "GCA013367975_00124" }, { "type": "cds", "sequence": "contig_1", "start": 74960, "stop": 75895, "strand": "-", "frame": 3, "gene": "arcC", "product": "Carbamate kinase", "db_xrefs": [ "COG:COG0549", "COG:E", "RefSeq:WP_036439257.1", "SO:0001217", "UniParc:UPI00051365B9", "UniRef:UniRef100_A0A454C933", "UniRef:UniRef50_P78030", "UniRef:UniRef90_D1J7K0" ], "nt": "ATGAGTAGAATTGTTATTGCATTAGGCGGAAACGCTTTAGGAAATAACCCCGAAGAACAAAAAGAACTTGTCAAAGTTCCTGCAGCCAAAATTGCTGAATTAGTAAAAGCAGGACACGAAGTTATTGTTGGACACGGAAACGGACCACAAGTTGGAATGATTTTCAATGGTTTCGTTAGTGCTCACTCAGTTAATCCTAAGTCTCCTTTAGTTCCATTGCCTGAAGCAGGTGCAATGAGCCAAGGATACATTGGATATCATATGGTTAATGCTATTACAAATGCATTTAAAAACGAAGGATTAAAAAACAAGGAAGTTTTATACATTTTAACTCAAACTCTTGTAGATCCAAAAGACCCTGCATTCCAAAATCCTACAAAACCAATTGGCCCATTCTTTGCAACAGAAGCAGAAGCAGTTGCTGCAAACCCAAATAGCACAATTGTTGAAGATGCAGGTAGAGGCTACAGAAAAGTTGTAGCATCTCCAAAACCATTAAACTTTGTTGGTATTAATCAAATTAAAAAAGCTATTGAATCAGGTGCTACCGTTATCGTTGGTGGTGGTGGTGGAATTCCTACTGTTGAAGATGAAAATGGCTTTATTAAAGGTGTAGATGGTGTTATTGACAAAGATTTCGCTTTAGCAAAAATGGCTGCATTAGCAAAAGCTGATTACTTTATTGTTCTAACAGCAGTTGATTATGTTAAAGTAAATTACAACAAGCCAGATCAAAAAGATCTAAAACACGCTACAAAAGCAGAACTTGAAAAATACATTGCTGAAAAACAATTTGCCCCAGGATCAATGTTACCAAAGGTACAAGCAGCTATTAAATTCGTTGAAGAAGGCGGAAAAGCAGCATTTATTGGTGATTTAAAAGATCTTGAAAACATTCTAGCAGAAAAAATCGGTACAAAAGTTACTTTAAATTAA", "aa": "MSRIVIALGGNALGNNPEEQKELVKVPAAKIAELVKAGHEVIVGHGNGPQVGMIFNGFVSAHSVNPKSPLVPLPEAGAMSQGYIGYHMVNAITNAFKNEGLKNKEVLYILTQTLVDPKDPAFQNPTKPIGPFFATEAEAVAANPNSTIVEDAGRGYRKVVASPKPLNFVGINQIKKAIESGATVIVGGGGGIPTVEDENGFIKGVDGVIDKDFALAKMAALAKADYFIVLTAVDYVKVNYNKPDQKDLKHATKAELEKYIAEKQFAPGSMLPKVQAAIKFVEEGGKAAFIGDLKDLENILAEKIGTKVTLN", "aa_hexdigest": "7d5aecb977527eca1dc65444c741f3ea", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00051365B9", "ncbi_nrp_id": "WP_036439257.1", "uniref100_id": "UniRef100_A0A454C933", "db_xrefs": [ "SO:0001217", "UniParc:UPI00051365B9", "RefSeq:WP_036439257.1", "UniRef:UniRef100_A0A454C933" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C933", "uniref90_id": "UniRef90_D1J7K0" }, "psc": { "uniref90_id": "UniRef90_D1J7K0", "gene": "ArcC", "product": "Carbamate kinase", "uniref50_id": "UniRef50_P78030", "cog_id": "COG0549", "cog_category": "E" }, "pscc": { "uniref50_id": "UniRef50_P78030", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78030" ], "product": "Carbamate kinase-like protein" }, "genes": [ "arcC" ], "id": "AHCKFKLKGB_165", "locus": "GCA013367975_00125" }, { "type": "cds", "sequence": "contig_1", "start": 75897, "stop": 76145, "strand": "-", "frame": 2, "gene": null, "product": "Ornithine carbamoyltransferase chain F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "nt": "ATGGCAATGATCAAAGCAGCAAAAGAAAATGTTATCTTCCTACACTGCTTACCAGCATTCCACGATGACCAAACTCAATTCTCAGCAACTATTAAAGAAACCTTAGGCGCAAAATACCCAGTTGTTTCAACCGGTGCTATGGAAGTTACTGATGAAGTATTCCAATCAAAATACAACAAATCAATCGAACAAGCTGGAAACCGTATGCACACAATTAAGGCTGTTATTCTAGCTACTTTAGGATACTAA", "aa": "MAMIKAAKENVIFLHCLPAFHDDQTQFSATIKETLGAKYPVVSTGAMEVTDEVFQSKYNKSIEQAGNRMHTIKAVILATLGY", "aa_hexdigest": "0e8f710bbe858e2fe23fe497b4f89f6b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4U9HLN2", "query_cov": 0.9634146341463414, "subject_cov": 0.9080459770114943, "identity": 0.519, "score": 75.1, "evalue": 4.39e-16, "valid": false, "product": "Ornithine carbamoyltransferase chain F", "uniref50_id": "UniRef50_A0A4U9HLN2" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9HLN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "product": "Ornithine carbamoyltransferase chain F" }, "genes": [], "id": "AHCKFKLKGB_166", "locus": "GCA013367975_00126" }, { "type": "cds", "sequence": "contig_1", "start": 76359, "stop": 76520, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00185.30" ], "nt": "ATGCTAGCTGACTACTTAACATTTAAAGAACACGTTGGAGACCTAAGAGGAAAGAAAATTGTTTTCTCAGGTGATATTAAAAACAACGTTGCTCGTTCATTAATGATTGGTGCTGCATTCTTTGGTGTTAATATTGTATTATGTGGACCAAAAGCTCAATGA", "aa": "MLADYLTFKEHVGDLRGKKIVFSGDIKNNVARSLMIGAAFFGVNIVLCGPKAQ", "aa_hexdigest": "d35d8a671afba032d3529a3fb2aa2a80", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00185.30", "name": "OTCace", "length": 36, "aa_cov": 0.6792452830188679, "hmm_cov": 1.0, "evalue": 3.837079794781791e-09, "score": 32.08402633666992, "start": 17, "stop": 52 } ], "seq_stats": { "molecular_weight": 5754.7484, "isoelectric_point": 9.428968620300292 }, "id": "AHCKFKLKGB_167", "locus": "GCA013367975_00127" }, { "type": "cds", "sequence": "contig_1", "start": 76554, "stop": 76940, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02729.26" ], "nt": "ATGCCATTAAATTTAAAGGGTCGTAGTTTAGACAGCGCTCTAAACTTTACAACCGAAGAAATTAACTATTTACTAGATTTATCACTAGAACTAAAAAGAGCAAAATTTGCTGGTTTACACACAAATAATCGCCCATTAGTTGGAAAAAACATTGTTATAATGTTCCAAAAAGACTCAACAAGAACTCGTTGTTCATTTGAAGTTGCAGCTGCTGATTTAGGTGCAAGCTGTACATACATTGGCCCAACAGGATCAAACTTTGGTAAAAAAGAATCAGTTATTGACACAGCTAAAGTTTTAGGCCAAATTTATGATGGTATTGAATTCCGTGGATTCAAACAATCAGATGTTGATGCATTAGTAAAATACTCAGGAATTCCTGTATGA", "aa": "MPLNLKGRSLDSALNFTTEEINYLLDLSLELKRAKFAGLHTNNRPLVGKNIVIMFQKDSTRTRCSFEVAAADLGASCTYIGPTGSNFGKKESVIDTAKVLGQIYDGIEFRGFKQSDVDALVKYSGIPV", "aa_hexdigest": "06307b4f1814626b0733c2ae428eb8cc", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BB40E1", "query_cov": 1.0, "subject_cov": 0.8152866242038217, "identity": 0.563, "score": 144.0, "evalue": 8.79e-42, "valid": false, "uniref50_id": "UniRef50_A0A3D1ABF9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02729.26", "name": "OTCace_N", "length": 126, "aa_cov": 0.9375, "hmm_cov": 0.9841269841269841, "evalue": 3.04592214726167e-30, "score": 100.5682373046875, "start": 9, "stop": 128 } ], "seq_stats": { "molecular_weight": 14021.913599999985, "isoelectric_point": 8.562320137023928 }, "id": "AHCKFKLKGB_168", "locus": "GCA013367975_00128" }, { "type": "cds", "sequence": "contig_1", "start": 77137, "stop": 77385, "strand": "-", "frame": 1, "gene": "mscL", "product": "Large-conductance mechanosensitive channel", "db_xrefs": [ "COG:COG1970", "COG:M", "KEGG:K03282", "SO:0001217", "UniRef:UniRef50_D1J7K2", "UniRef:UniRef90_D1J7K2" ], "nt": "TTGGGTGCATTAATTCAATTTGTTATTGTTTCAACATTTATTTTTATAGGATTAATGATTGTGTTTACAATTAAGAATTCTATTCTATATGCAAAAGCCAAAAAGAACCCAATTGAAGAAGAAATAAAGGAAGAACCTACTCCAACAACAGAAGAATTAATTTTAAATGAATTAAAAAAATTAAATGAAAATTTATTAAAAACTAAAAATTCTGGGAATAAAAAAGATTTAAAAAATGATTTAAAATAA", "aa": "MGALIQFVIVSTFIFIGLMIVFTIKNSILYAKAKKNPIEEEIKEEPTPTTEELILNELKKLNENLLKTKNSGNKKDLKNDLK", "aa_hexdigest": "ec04fc70b29e801127da649899a760df", "start_type": "TTG", "rbs_motif": null, "genes": [ "mscL" ], "truncated": "3-prime", "pseudogene": { "start": 77137, "stop": 77682, "observations": { "mutation": [ 77439, 77448 ], "stop": [ 77439, 77448 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K2", "query_cov": 1.0, "subject_cov": 0.4293193717277487, "identity": 0.927, "score": 136.0, "evalue": 5.86e-39, "gene_start": 1, "gene_end": 82, "reference_start": 110, "reference_end": 191, "reference_sequence": "FLLIKKTKTRIKFILYNIDMTSKELEEKKHYIKKSYVDAKKIVSRGNMFMLAIGLLLGASFGALVSSLANDVIMSAITKAIGMKNLDAWVVWPGIHATKETGGIFIGKFLGALIQFIIVSTFIFIGLMIVFTIKNSILYAKAKKSPIEEEIKEEPTPTTEELILNELKKLNENLLETKNSGDKKDLKNNLK" }, "paralog": false, "identity": 0.9392265193370166, "score": 329.0, "evalue": 1.81e-117, "description": "Internal stop codon at 77439, 77448. Nonsense mutation around 77439, 77448.", "psc": { "uniref90_id": "UniRef90_D1J7K2", "gene": "MscL", "product": "Large-conductance mechanosensitive channel", "uniref50_id": "UniRef50_D1J7K2", "cog_id": "COG1970", "cog_category": "M", "kegg_orthology_id": "K03282", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K2", "UniRef:UniRef50_D1J7K2", "COG:COG1970", "COG:M", "KEGG:K03282" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7K2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7K2" ], "product": "Large-conductance mechanosensitive channel" } }, "id": "AHCKFKLKGB_169", "locus": "GCA013367975_00129" }, { "type": "cds", "sequence": "contig_1", "start": 77446, "stop": 77655, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01741.23" ], "nt": "ATGACAAGTAAAGAATTAGAAGAAAAAAAGCATTATATTAAAAAGTCATATATAGATGCAAAAAAAATAGTTTCGCGTGGAAACATGTTTATGCTAGCAATTGGGCTTTTATTAGGAGCTTCATTTGGGGCACTAGTATCATCACTAGCAAATGATGTTATTATGTCAGCAATAACAAAAGCAGTCGGTATGAAAAATCTTGATGCTTGA", "aa": "MTSKELEEKKHYIKKSYIDAKKIVSRGNMFMLAIGLLLGASFGALVSSLANDVIMSAITKAVGMKNLDA", "aa_hexdigest": "f66c04029516805ebdcad7631d08d727", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01741.23", "name": "MscL", "length": 47, "aa_cov": 0.6811594202898551, "hmm_cov": 1.0, "evalue": 5.775383942648525e-13, "score": 44.62319564819336, "start": 20, "stop": 66 } ], "seq_stats": { "molecular_weight": 7462.8359999999975, "isoelectric_point": 9.573893547058105 }, "id": "AHCKFKLKGB_170", "locus": "GCA013367975_00130" }, { "type": "tRNA", "sequence": "contig_1", "start": 77728, "stop": 77820, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 68.9, "nt": "GGGTAAGTACTCAAGTGGTCGAAGAGGCGGTCCTGCTAAGACTGTAGGGGTGCTAAAACACCCGCGGAGGTTCAAATCCTCTCTTACCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 77784, 77786 ], "id": "AHCKFKLKGB_33", "locus": "GCA013367975_00131" }, { "type": "cds", "sequence": "contig_1", "start": 77888, "stop": 78055, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTATATTAGCATAGATCCTCAAAGCAATTATTATACAAAGAACATTGACTACTACAAGCAATTGCAAGAAGCTTCTAATCATGCTAAAAATTTAAAATTAGGAATTTTTGATAGTGGCAATGATATCAATAAAATTTACCCTCCAAGATCAAGGAAAAATTAA", "aa": "MYYISIDPQSNYYTKNIDYYKQLQEASNHAKNLKLGIFDSGNDINKIYPPRSRKN", "aa_hexdigest": "9df24ca2bbb1d30e9a19b08ce0ae9de5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6532.224200000001, "isoelectric_point": 9.260254859924316 }, "id": "AHCKFKLKGB_171", "locus": "GCA013367975_00132" }, { "type": "cds", "sequence": "contig_1", "start": 78212, "stop": 78568, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATCAAGAATAAAAAAACTTACTAAGATAGCTAAGCCAAATAATCAAAACGACACAAATAATGACAAATTTAACATCAATAATTTTCATGAAGCTAAATTTGATTTTGAAATACTAAAAAATAATAAATTTGAAGTAGGAAAAGCAACAGAAATAAATATTGCAAGGATTGCTGATGGAGATACAATATCAGATTCTTATCGAAACAAATATCGCTTTAATGGAATTGATACGCCGGAAACTCATAAAAAGAGTAATGGCAAATTTGAAGAAACATCCGGCATTCAATATAAATATGGAAAAATTGCCGAAAATTATACAAAGAATTTTCTAAACAATGCTGCTAAAATTTGA", "aa": "MLSRIKKLTKIAKPNNQNDTNNDKFNINNFHEAKFDFEILKNNKFEVGKATEINIARIADGDTISDSYRNKYRFNGIDTPETHKKSNGKFEETSGIQYKYGKIAENYTKNFLNNAAKI", "aa_hexdigest": "30095884925c3f7508b4d815c9e366fd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13628.078899999979, "isoelectric_point": 9.467649650573733 }, "id": "AHCKFKLKGB_172", "locus": "GCA013367975_00133" }, { "type": "cds", "sequence": "contig_1", "start": 78587, "stop": 78841, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAAATACTAAAAAAATATTGAATCTCACATTGGGCTCTATTGCTACAATTTTGCCAATTTCATTAATTTCATATTCTTGTAAGCTAGAAAAAATTAATGACAAGGAACAACAAAATTTTAAAGAAAAATATGACAAAAAAATAAAGGAAATTGAAAATAGCAATGAATGGCAAAAAAATGATTATTATAAACAATTAATTGCTGATGCCAAAAAATTAATAAATTCAAATAGCAAAGAAGTTAATTGA", "aa": "MERNTKKILNLTLGSIATILPISLISYSCKLEKINDKEQQNFKEKYDKKIKEIENSNEWQKNDYYKQLIADAKKLINSNSKEVN", "aa_hexdigest": "3ddba50fe502c357907029b94a1bddf6", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9880.226099999993, "isoelectric_point": 9.162778663635255 }, "id": "AHCKFKLKGB_173", "locus": "GCA013367975_00134" }, { "type": "cds", "sequence": "contig_1", "start": 78825, "stop": 79016, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCATTAAAAAAATAAATTTCAATACCGAGTTGCAAATTGCCAATGCAAAAGCCCTAAATGATTTTTTAAAAAATAACAATTTTTTAGAAAACAAAAATTACAATCCAAGAATTCTTTATGCAAATTCAAATCAAGCAATATATAAATGTACGTTGGACTTATTGAATAAATATGGAAAGAAATACTAA", "aa": "MGIKKINFNTELQIANAKALNDFLKNNNFLENKNYNPRILYANSNQAIYKCTLDLLNKYGKKY", "aa_hexdigest": "5e0d467dac9d0744d89122546c427103", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7374.414200000003, "isoelectric_point": 9.630238914489745 }, "id": "AHCKFKLKGB_174", "locus": "GCA013367975_00135" }, { "type": "cds", "sequence": "contig_1", "start": 79128, "stop": 79580, "strand": "-", "frame": 2, "gene": "fba", "product": "Fructose-bisphosphate aldolase", "db_xrefs": [ "COG:COG0191", "COG:G", "SO:0001217", "UniRef:UniRef50_A0A3B0NXB7", "UniRef:UniRef90_D1J7K4" ], "nt": "TTGGCAACAAAGGAGGCAAATTTAGATATCATACTTGGAGCTTCTCCTTCTGCAATTAAATACATGGGCGGATATAAAACCGTTGTCAACTTAGTTAACGGTCTTATTCAAGATTTAGAAATTAAAACTAATGTTATATTGCACTTAGATCATGGTAATTATAACGATTGTATTAATGCTATAAATGCCGGCTTTAAGTCAATAATGTATGATGGTAGTAAAGAAGATTTTAAAACCAATATATTGAACACAAAAAAAATAGTTAACATTGCAAAAAAAATGGATATAACTGTTGAAGCAGAAGTAGGTAGGATCGGACTAGATATTAGTAAAAATAAATCACTTGATATTCAAAAAACAAATATTGAAGATGCTAAAAAATTAGCAAATACCGGAATTGATTTATTAGCAGTGGCAATTGGAAATATTCATGGTCAATATGAAAGTAACTGA", "aa": "MATKEANLDIILGASPSAIKYMGGYKTVVNLVNGLIQDLEIKTNVILHLDHGNYNDCINAINAGFKSIMYDGSKEDFKTNILNTKKIVNIAKKMDITVEAEVGRIGLDISKNKSLDIQKTNIEDAKKLANTGIDLLAVAIGNIHGQYESN", "aa_hexdigest": "7ec99fa5980480b6ba96b54b5c1b5677", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "genes": [ "fba" ], "truncated": "both", "pseudogene": { "start": 78828, "stop": 79709, "observations": { "mutation": [ 79130, 79598 ], "stop": [ 79130, 79598 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K4", "query_cov": 1.0, "subject_cov": 0.5102040816326531, "identity": 0.98, "score": 288.0, "evalue": 2.49e-96, "gene_start": 1, "gene_end": 150, "reference_start": 44, "reference_end": 193, "reference_sequence": "MIKLKRKNMEIQNLNQTLKKIKEENYAILHININNLEWAKFVLLATKQANLDIILGASPSAIKYMGGYKTVVNLVNGLIQDLEIKTNVILHLDHGNYNDCINAINAGFKSIMYDGSKEDFETNILNTKKIVNIAKKMDITVEAEVGRIGLDISKNKSLDIQKTNIEDAKKLANTGIDLLAVAIGNIHGQYESNWKGLDFELLDKLNQEIKIPLVLHGASGIKEDDIKKAIRLGIKKINFNTELQIANAKALNDFLKNNNFLENKNYNPRILYANSNQAIYKCTLDLLNKYGKKN" }, "paralog": false, "identity": 0.9761092150170648, "score": 559.0, "evalue": 9.16e-206, "description": "Internal stop codon at 79130, 79598. Nonsense mutation around 79130, 79598.", "psc": { "uniref90_id": "UniRef90_D1J7K4", "gene": "Fba", "product": "Fructose-bisphosphate aldolase", "uniref50_id": "UniRef50_A0A3B0NXB7", "cog_id": "COG0191", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K4", "UniRef:UniRef50_A0A3B0NXB7", "COG:COG0191", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NXB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NXB7" ], "product": "Fructose-bisphosphate aldolase" } }, "id": "AHCKFKLKGB_175", "locus": "GCA013367975_00136" }, { "type": "cds", "sequence": "contig_1", "start": 79906, "stop": 80016, "strand": "-", "frame": 1, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "EC:2.7.7.6", "GO:0003899", "GO:0006351", "SO:0001217", "UniRef:UniRef50_N9VBR1", "UniRef:UniRef90_D1J7K5" ], "nt": "ATGGAATATAAATCGCAAGTTGATATAGCTAAAGAAATTTTAGATAGACATGATGCACTAGATTTTGAAACTTTATTTAATTATGTAAAAGACGAACTACTTGAACGTTGA", "aa": "MEYKSQVDIAKEILDRHDALDFETLFNYVKDELLER", "aa_hexdigest": "c8d54175b4f84f2b8947c9e2089e7878", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 79759, "stop": 80016, "observations": { "mutation": [ 79791, 79908 ], "stop": [ 79791, 79908 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K5", "query_cov": 1.0, "subject_cov": 0.4235294117647059, "identity": 1.0, "score": 72.8, "evalue": 5.06e-16, "gene_start": 1, "gene_end": 36, "reference_start": 1, "reference_end": 36, "reference_sequence": "MEYKSQVDIAKEILDRHDALDFETLFNYVKDELLERWKEELTSSISDEELLIKKRGELYKLLTIDGRFFKKVNGLWTSKRPEFKN" }, "paralog": false, "identity": 0.9764705882352941, "score": 162.0, "evalue": 8.64e-54, "description": "Internal stop codon at 79791, 79908. Nonsense mutation around 79791, 79908.", "psc": { "uniref90_id": "UniRef90_D1J7K5", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_N9VBR1", "go_ids": [ "GO:0003899", "GO:0006351" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K5", "EC:2.7.7.6", "UniRef:UniRef50_N9VBR1", "GO:0003899", "GO:0006351" ] }, "pscc": { "uniref50_id": "UniRef50_N9VBR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VBR1" ], "product": "HTH HARE-type domain-containing protein" } }, "id": "AHCKFKLKGB_176", "locus": "GCA013367975_00137" }, { "type": "cds", "sequence": "contig_1", "start": 80468, "stop": 81070, "strand": "+", "frame": 2, "gene": null, "product": "arginine deiminase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005698E2F" ], "nt": "ATGTCTGTATTTGACAGTAAATTTAATGGCATTCACGTTTATTCAGAAATCGGAGAACTAGAAACTGTTCTAGTTCACGAACCTGGACGTGAAATTGACTACATTACACCAGCTCGTTTAGACGAATTACTATTCTCAGCTATTCTAGAAAGCCACGATGCTAGAAAAGAACACCAATCATTTGTTAAAATAATGAAGGACAGAGGAATCAACGTTGTTGAATTAACTGACCTAGTTGCTGAAACATATGATTTAGCATCAAAAGCAGCTAAAGAAGAATTCATTGAAACATTCTTGGAAGAAACAGTTCCTGTTTTAACAGAAGCAAACAAAAAAGCTGTAAGAGCATTCTTATTAAGCAAACCAACTCATGAAATGGTTGAATTCATGATGTCAGGTATTACTAAATATGAATTAGGAGTTGAATCAGAAAACGAACTAATCGTTGACCCAATGCCTAACTTATACTTTACACGTGACCCATTTGCATCAGTAGGTAATGGTGTTACAATTCACTTTATGCGTTACATCGTAAGACGCCGTGAAACACTATTTGCAAGATTTGTATTTAGAAATCACCCTAAACTAGTTAAAACTCCATGA", "aa": "MSVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTP", "aa_hexdigest": "a1b2024decd2b61298954f3300560145", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0005698E2F", "query_cov": 0.915, "subject_cov": 0.9104477611940298, "identity": 0.574, "score": 216.0, "evalue": 3.11e-68, "valid": false, "product": "arginine deiminase family protein", "uniref50_id": "UniRef50_UPI0005698E2F" }, "pscc": { "uniref50_id": "UniRef50_UPI0005698E2F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005698E2F" ], "product": "arginine deiminase family protein" }, "genes": [], "id": "AHCKFKLKGB_177", "locus": "GCA013367975_00138" }, { "type": "cds", "sequence": "contig_1", "start": 81086, "stop": 81256, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGTCAATTGAAGGTGGTGACGTATTTATCTACAACAACGAAACATTAGTTGTTGGTGTTTCAGAAAGAACTGACCTAGATACTATTACTTTATTAGCTAAAAACATCAAGGCAAACAAAGACGTTGAATTTAAACGTATTGTTGCAATCAATGTTCCTAAATGA", "aa": "MKMSIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKDVEFKRIVAINVPK", "aa_hexdigest": "756abf8800f3830929735dd4d49184ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6252.2179000000015, "isoelectric_point": 6.125447273254394 }, "id": "AHCKFKLKGB_178", "locus": "GCA013367975_00139" }, { "type": "cds", "sequence": "contig_1", "start": 81401, "stop": 81694, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCACTAGACAAATTATTAGCTTCAATCATTAACAAAGAACCAGTTTTAATTCCTATTGGTGGTGCTGGAGCAACTGAAATGGAAATTGCTCGTGAAACAAACTTTGACGGAACCAACTACCTAGCAATTAAACCAGGACTTGTTATTGGTTATGACCGTAACGAAAAAACAAATGCTGCTTTAAAAGCTGCTGGTATTACTGTATTGCCATTCCACGGAAACCAACTATCTCTAGGTATGGGAAATGCACGTTGCATGTCAATGCCTCTATCACGTAAGGATGTTAAGTGA", "aa": "MPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVK", "aa_hexdigest": "7c5eac652468c5e656c36d6582d9a4e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10354.017699999995, "isoelectric_point": 8.851718711853028 }, "id": "AHCKFKLKGB_179", "locus": "GCA013367975_00140" }, { "type": "cds", "sequence": "contig_1", "start": 81811, "stop": 82107, "strand": "-", "frame": 1, "gene": "dut2", "product": "dUTP diphosphatase", "db_xrefs": [ "COG:COG4508", "COG:F", "SO:0001217", "UniRef:UniRef50_D1J7K7", "UniRef:UniRef90_D1J7K7" ], "nt": "ATGTTAGAAGAATATGCGGATGGAATTCATTTTATTACTAGTATCGCATTAAAAAATAACATAACCTCATTTGATATAAAGCCAATAATTAAATATAATGACTTTACCTTGCAACTTGCTTATGCATATCAATGCTTTACCAAATTGTTTTTGCACCATAATAAAAGATTAATAAAAAAAGCATATGGTGTTTATTTGGGATTAGGTGAAATTATGAATATTGATCGCGATATGATTTGTACGGCCTATGCCAAAAAAAATCATAAAAACTATGAAAGAATAAAAAATAATTATTAA", "aa": "MLEEYADGIHFITSIALKNNITSFDIKPIIKYNDFTLQLAYAYQCFTKLFLHHNKRLIKKAYGVYLGLGEIMNIDRDMICTAYAKKNHKNYERIKNNY", "aa_hexdigest": "b29315dcbb54fbe93b236ab84ddd5618", "start_type": "ATG", "rbs_motif": null, "genes": [ "dut2" ], "truncated": "3-prime", "pseudogene": { "start": 81811, "stop": 82338, "observations": { "mutation": [ 82140 ], "stop": [ 82140 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K7", "query_cov": 1.0, "subject_cov": 0.56, "identity": 0.99, "score": 202.0, "evalue": 4.97e-65, "gene_start": 1, "gene_end": 98, "reference_start": 78, "reference_end": 175, "reference_sequence": "MELFLTFKFIIQNMNLTEVFEAQKKLDLAFASSIEKTEFLNIKIKMVIALLVELGEFANEVKLFKYWKKDKSINQISMLEEYADGIHFITSIALKNNITSFDIKPIIKYNDFTLQLAYTYQCFTKLFLHHNKRLIKKAYGVYLGLGEIMNIDRDMICTAYAKKNHKNYERIKNNY" }, "paralog": false, "identity": 0.9828571428571429, "score": 342.0, "evalue": 8.88e-123, "description": "Internal stop codon at 82140. Nonsense mutation around 82140.", "psc": { "uniref90_id": "UniRef90_D1J7K7", "gene": "Dut2", "product": "dUTP diphosphatase", "uniref50_id": "UniRef50_D1J7K7", "cog_id": "COG4508", "cog_category": "F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K7", "UniRef:UniRef50_D1J7K7", "COG:COG4508", "COG:F" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7K7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7K7" ], "product": "dUTP diphosphatase" } }, "id": "AHCKFKLKGB_180", "locus": "GCA013367975_00141" }, { "type": "cds", "sequence": "contig_1", "start": 82138, "stop": 82338, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08761.16" ], "nt": "ATGGAATTATTTTTAACTTTTAAGTTTATAATTCAAAACATGAATTTAACAGAAGTTTTTGAAGCCCAAAAGAAATTAGACCTTGCATTTGCAAATAGTATTGAAAAAACAGAATTTTTGAATATCAAAATCAAAATGGTTATTGCTTTGCTTGTTGAACTAGGAGAATTTGCAAACGAAGTAAAACTATTTAAATATTGA", "aa": "MELFLTFKFIIQNMNLTEVFEAQKKLDLAFANSIEKTEFLNIKIKMVIALLVELGEFANEVKLFKY", "aa_hexdigest": "616d1077d09d512230a2b21d98a99e41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08761.16", "name": "dUTPase_2", "length": 52, "aa_cov": 0.7878787878787878, "hmm_cov": 1.0, "evalue": 2.793892880856648e-10, "score": 36.042789459228516, "start": 15, "stop": 66 } ], "seq_stats": { "molecular_weight": 7762.191499999994, "isoelectric_point": 5.319015693664551 }, "id": "AHCKFKLKGB_181", "locus": "GCA013367975_00142" }, { "type": "tRNA", "sequence": "contig_1", "start": 82373, "stop": 82456, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 69.5, "nt": "GCCCCGATGGCGGAATGGTAGACGCAGTTGACTCAAAATCAACCGGTGAGAGCCATGCTGGTTCAAGTCCAGTTCGGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 82406, 82408 ], "id": "AHCKFKLKGB_3", "locus": "GCA013367975_00143" }, { "type": "cds", "sequence": "contig_1", "start": 82694, "stop": 83431, "strand": "+", "frame": 2, "gene": null, "product": "RDD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TR13", "UniRef:UniRef90_D1J7K8" ], "nt": "TTGCTAATATCTGCAATTGGTTTACTTGTTTTAATTTATATTACATTGGTCTTTAAAAACAAATATGAAGAATCTCTTGAACTTGAAATTTTTAATGATATTTTTGTAAGTTGTTTAGTGGGAATATTTTCAGGAGCATTATTAGTCTTGGCTTCTTTTATATTATTAGATGTTTTTAAAAGAAATAAAATCAAAAATTTCTTTGAATATTATTGTTATCTTTCTTCATTAAAAAGTAAAAATAAATTTTTATTTTTTAAAAATTATGATTTAAGAGAAATAATAAATAATAAAAATCAACTAACCAAACAAGAATTTATTTGCTTTGTTGCCGAAAAATTAAACTATACAATGCCTTCTATTGAATATAAAAACTTAGTTAATGAAATTAATAGCGATTTTGCCAAACATTCATTTTTAGATCCAAATTTTAAAAAGCAAAAGAAAGCAACACTTACTAGGTTAATTATCTTTGAAATTGTCATTCCTTTATTTATTTCTATTGCATTAATAGTATTAATTGCATGTCTTTTTAACAATGATGATCAACCAATCAGGGCAATTTATAGATTGATAATCATTTTAGTCAACGCAATAATTGTTTTAAGCCTATCGATTTTAATTTATGAACTATTTATTTTAAGTAAAGTAAAAAATTATCAAAATTTCAATAATTTATATTTGCTTTCATTTAATAATTACTCATATAAGTATTTAAATTCTAGTTTAGTAAGATAG", "aa": "MLISAIGLLVLIYITLVFKNKYEESLELEIFNDIFVSCLVGIFSGALLVLASFILLDVFKRNKIKNFFEYYCYLSSLKSKNKFLFFKNYDLREIINNKNQLTKQEFICFVAEKLNYTMPSIEYKNLVNEINSDFAKHSFLDPNFKKQKKATLTRLIIFEIVIPLFISIALIVLIACLFNNDDQPIRAIYRLIIILVNAIIVLSLSILIYELFILSKVKNYQNFNNLYLLSFNNYSYKYLNSSLVR", "aa_hexdigest": "0fd42c1b2364af87f3670be02ccaa4f4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7K8", "query_cov": 1.0, "subject_cov": 0.9074074074074074, "identity": 0.98, "score": 441.0, "evalue": 2.67e-155, "valid": true, "uniref50_id": "UniRef50_N9TR13" }, "pscc": { "uniref50_id": "UniRef50_N9TR13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TR13" ], "product": "RDD domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_182", "locus": "GCA013367975_00144" }, { "type": "cds", "sequence": "contig_1", "start": 83793, "stop": 84002, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGATAAACAAAATTCTTTTAGGGCTAGCACCTGCTGCAGCACTTATTGCTGCATCAGGACTTTCTGCTGCTTGTGGTCATACAGGAACCGGCTATGGATTTGACCAAACAGATGATGGAAAATTAGTATTAGCCTCAGGATTTTCTAACACAAATAAACAAGGTATTGCCTTAAAAGCAGTTGTTGATGCATACAACAAATGA", "aa": "MKKINKILLGLAPAAALIAASGLSAACGHTGTGYGFDQTDDGKLVLASGFSNTNKQGIALKAVVDAYNK", "aa_hexdigest": "547e5a70071c34812bb968a23cc6df27", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6951.932700000004, "isoelectric_point": 9.25226078033447 }, "id": "AHCKFKLKGB_183", "locus": "GCA013367975_00145" }, { "type": "cds", "sequence": "contig_1", "start": 84057, "stop": 84281, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAATGGATATAATACTAACACTTTAAGAAGTGACTTGGATGCTAAAAACTCAAATGACTTTTATAACATGACATTAAATTATCCAGCTGCTGCTTCGATATTGTCTCAATACAAAATGAATTTAGCTATTTCAAAAGAAGATTATGATAAATTCGGTATTGAAAAAGGATTTAATTTGGGTAATGATTTAATTGGTGGAAACACTAAAAAAGAAAAATGA", "aa": "MANGYNTNTLRSDLDAKNSNDFYNMTLNYPAAASILSQYKMNLAISKEDYDKFGIEKGFNLGNDLIGGNTKKEK", "aa_hexdigest": "a90dd48672fece00390f31cd8469b741", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8262.105099999997, "isoelectric_point": 6.115898323059081 }, "id": "AHCKFKLKGB_184", "locus": "GCA013367975_00146" }, { "type": "cds", "sequence": "contig_1", "start": 84309, "stop": 84464, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAGTTGCTAAGGCATTAGTAGGAAAATTCATTAAAGAATTAAAAGACTTGGGTGTAAAAATTTCTGACAAAGACAATACTAAAGTTAACGAATATCTAAAATATTACACAGAAAATACAAATGAAGCAAAAGGTGTTGATGAAACTTGA", "aa": "MLSVAKALVGKFIKELKDLGVKISDKDNTKVNEYLKYYTENTNEAKGVDET", "aa_hexdigest": "5d214cb6440ddbe41660613eeaa4592c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5752.501400000002, "isoelectric_point": 6.161596870422362 }, "id": "AHCKFKLKGB_185", "locus": "GCA013367975_00147" }, { "type": "cds", "sequence": "contig_1", "start": 84528, "stop": 84839, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATCAAATCTTTAGAGATTATTCAAGTATGATTAATTTCTCAATTCAAGCAAAGAAAATGTACAACAAAGATCCTTTATTAAATGTTATTGGTTTTGACTCACTACCAAATGTTATTAATACAATGACAACTTCATTAACCAATGGAGATATTAATAAAGGATATATAAAACCAGATGAAGATAAAATAACAACAGGTGGATTTGACTACTCAACATTTCTAAAAGATGCAAATAGTGAACAATCAAAATTATTCAAAAGGATAGTTGACTTAATCCTAGATGGTGTTAAGGAAGGCGCTGTTTGA", "aa": "MSDQIFRDYSSMINFSIQAKKMYNKDPLLNVIGFDSLPNVINTMTTSLTNGDINKGYIKPDEDKITTGGFDYSTFLKDANSEQSKLFKRIVDLILDGVKEGAV", "aa_hexdigest": "b1a54264c073f023b626485609dc78e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11550.994299999988, "isoelectric_point": 4.944901466369628 }, "id": "AHCKFKLKGB_186", "locus": "GCA013367975_00148" }, { "type": "cds", "sequence": "contig_1", "start": 84894, "stop": 85040, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCTTGGATCAACCGCCGGATATTCACATACATTCTCAAACGCTCAATCATTAACAAACTTAAAAATAAAAGGCACAACTACTTCATTAGATCAATCTACATTGGAAGTTTTAGGCCAAAAACCTAAAGAATCTGACAAATGA", "aa": "MSLGSTAGYSHTFSNAQSLTNLKIKGTTTSLDQSTLEVLGQKPKESDK", "aa_hexdigest": "e01ef7ed911dbc6f88861c21110498ce", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5101.610800000001, "isoelectric_point": 8.120776176452637 }, "id": "AHCKFKLKGB_187", "locus": "GCA013367975_00149" }, { "type": "cds", "sequence": "contig_1", "start": 85503, "stop": 85892, "strand": "+", "frame": 3, "gene": null, "product": "Putative mycoplasma lipoprotein, C-terminal region", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215C1230", "UniRef:UniRef90_UPI00215C1230" ], "nt": "ATGTTCCAACACGATTTAGAAAACTTTGAAATTGAAGAAGTATTTAAAGGAAAAACTACAACCACAAAATTCGACGGCAAGCAAAAACCTATTGACATTTTCAATGAATACGGAAGCTATATTTCACCAACTAAGTCATACTTCTCATCAGATAAAGCAAGCAAATTAAATACAGCAACAAAAATAGCATTTGACAACTTTAAACAACTTGACAATAAAGATAGTGGTTATATCCCATCAGAAGATATTGCAACTTCATTATCAGATGTTTTAAGAGAAGCAATAACAAGTGCTGGAAAAGCAGCGTTATCAAAAGCAAACGTTAACGAAGTATTTGAATATAAGGAATTTGTTTCAAAAATTTTAGGTCAATTAAGTAACCAACTTTAA", "aa": "MFQHDLENFEIEEVFKGKTTTTKFDGKQKPIDIFNEYGSYISPTKSYFSSDKASKLNTATKIAFDNFKQLDNKDSGYIPSEDIATSLSDVLREAITSAGKAALSKANVNEVFEYKEFVSKILGQLSNQL", "aa_hexdigest": "53e048157990d5c0453d658dd2475165", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 85335, "stop": 85892, "observations": { "mutation": [ 85377, 85500 ], "stop": [ 85377, 85500 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215C1230", "query_cov": 1.0, "subject_cov": 0.6972972972972973, "identity": 0.946, "score": 237.0, "evalue": 4.88e-78, "gene_start": 1, "gene_end": 129, "reference_start": 57, "reference_end": 185, "reference_sequence": "MVSSNKILNEADADWISSPLAFDKNAKKAVFIQGPSILPIHANEKEDKATKLFINWMFQHDLENFKIEEVFKGKTTTKTFEGKQKPIDIFNEYGSYISPTKSYFSSDKASKLNKATKIAFDNFKQLDKEDSGYIPSEDIATSLSDVLREAITSAGKAALSKANVNEVFEYKEFVSKILGQLSNQL" }, "paralog": false, "identity": 0.9351351351351351, "score": 336.0, "evalue": 1.08e-119, "description": "Internal stop codon at 85377, 85500. Nonsense mutation around 85377, 85500.", "psc": { "uniref90_id": "UniRef90_UPI00215C1230", "product": "Putative mycoplasma lipoprotein, C-terminal region", "uniref50_id": "UniRef50_UPI00215C1230", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215C1230", "UniRef:UniRef50_UPI00215C1230" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215C1230", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215C1230" ] } }, "id": "AHCKFKLKGB_188", "locus": "GCA013367975_00150" }, { "type": "cds", "sequence": "contig_1", "start": 85980, "stop": 86519, "strand": "+", "frame": 3, "gene": "yncB", "product": "TNase-like domain-containing protein", "db_xrefs": [ "COG:COG1525", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J7L0", "UniRef:UniRef90_D1J7L0" ], "nt": "ATGAAAAAATACAATAAATTTTTATTAATTTTAGCCTCATCTGCTGGCTTAGTGGTCGGGGGTCCGCTACTATCTGTATCATGCAAGACAGTGGATAAAGAAAAAATAAATTTATCAAAATTTGATGTAATAAAGAAAAATTATGAAATTCCAGTATATGAATTTTCAAAAGTGGGAACAATTTTTAATAAATCAATAAATTATAGAATGTATGTCACTGATGAAAAAGGAAATAAAACTATTAGAGAAGTTTCTCAATCATTATGGGATTATTATAATTACATTGAAGGCGAAGTTACTAGAATTTTTGATGGCGACACCATTGAAATAAAAGTAACAAAGCAACCAAATCCTAAACCTGGTGGTAAAGTTCTTAGTGTTCCTACAACAATGAGATTAAGAATACCAATGATTGATACATTGGAAGAAAATACGCCAGAAGTTGGAAAAGAAGAAAAGGAATTAGCAAAAAAAGATCATGCATATGCCGAAAAATTAATTCCTATTGGTTCAAAAGTTAGAGTAATTTCAAGCAATTGA", "aa": "MKKYNKFLLILASSAGLVVGGPLLSVSCKTVDKEKINLSKFDVIKKNYEIPVYEFSKVGTIFNKSINYRMYVTDEKGNKTIREVSQSLWDYYNYIEGEVTRIFDGDTIEIKVTKQPNPKPGGKVLSVPTTMRLRIPMIDTLEENTPEVGKEEKELAKKDHAYAEKLIPIGSKVRVISSN", "aa_hexdigest": "c8876c9e627e2f7915dea70255bf26ae", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "yncB" ], "truncated": "3-prime", "pseudogene": { "start": 85980, "stop": 86822, "observations": { "mutation": [ 86517 ], "stop": [ 86517 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L0", "query_cov": 1.0, "subject_cov": 0.5559006211180124, "identity": 0.972, "score": 343.0, "evalue": 3.76e-117, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MKKYNKFLLILASSAGLVVGAPLLSVSCKTVDKEKINLSKFDVIKKNYEIPVYEFSKVGTIFNKSINYRMYVTDEKGDKTIKEVSQSLWDYYNYIEGEVTRIFDGDTIEIKVTKQPNPKPGGKVLNVPTTMKLRIPMIDTLEENTPEVGKEEKELAKKDHAYAEKLIPIGSKVRVISSNWSEKTYDRFVGYVFFGKNFERQFDIEMLAGGYTLARLDTTKVISEFIADLSKESTEAKEIRSYLLPYAAYAINYGILNRKGFYGEVANMRSPYELSSKYLEHGQGMVDFSLSILHYNYWKKPKLATKKNNIYRYLEQINQNKK" }, "paralog": false, "identity": 0.9785714285714285, "score": 544.0, "evalue": 6.95e-199, "description": "Internal stop codon at 86517. Nonsense mutation around 86517.", "psc": { "uniref90_id": "UniRef90_D1J7L0", "gene": "YncB", "product": "TNase-like domain-containing protein", "uniref50_id": "UniRef50_D1J7L0", "cog_id": "COG1525", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L0", "UniRef:UniRef50_D1J7L0", "COG:COG1525", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L0" ], "product": "TNase-like domain-containing protein" } }, "id": "AHCKFKLKGB_189", "locus": "GCA013367975_00151" }, { "type": "cds", "sequence": "contig_1", "start": 86595, "stop": 86873, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCAGGTGGATATACTCTTGCTAGATTAGATACAACAAAAGTTATTTCAGAATTTATTGCTGATTTATCAAAAGAAAGCACTGAAGCAAAGGAAATAAGAAGTTACTTGTTGCCTTATGCTGCATATGCAATTAATTATGGAATTTTAAATAGAAAAGGATTTTATGGTGAAGTTGCCAACATGCGTTCTCCATATGAACTAAGTTCAAAATATTTGGAACACGGACAAGGTATGGTTGATTTCTCACTTTCAATACTTCATTATAACTATTGA", "aa": "MLAGGYTLARLDTTKVISEFIADLSKESTEAKEIRSYLLPYAAYAINYGILNRKGFYGEVANMRSPYELSSKYLEHGQGMVDFSLSILHYNY", "aa_hexdigest": "8df4e3bfd313c4224aaa41d011cbb95d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10429.735599999998, "isoelectric_point": 6.0617876052856445 }, "id": "AHCKFKLKGB_190", "locus": "GCA013367975_00152" }, { "type": "cds", "sequence": "contig_1", "start": 86960, "stop": 87358, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAAAGAAACAAAATCAAGAGAAGAATTATTAAAAGATATTCATAGTTTTTCTAAGCAACGTCATGAAATTAAAAAGACATTGCCTGCAATTGAAATTAAAAATTTAGTTATTGACTTTGGTGAATCACTAGCAGTCGATAATGCTTCATTTTGCATTAATAAAGGAGAATTAGTAACATTATTAGGACCATCTGGTTCTGGTAAAACGACTACCTTGAATGCCATTGCGGGACTTTTAAGACCAACAGGAGGAAAAATTTTCTTTTCAGGAATTGATGTAACAAAATTCTCACCTCAACAAAGAGAATTAGGATTGGTGTTTCAAAACTATGCACTATATCCTCACATGTCAGTTTATGAAAATATTGCATTTCCTCTATACAATGACAAACATTGA", "aa": "MKETKSREELLKDIHSFSKQRHEIKKTLPAIEIKNLVIDFGESLAVDNASFCINKGELVTLLGPSGSGKTTTLNAIAGLLRPTGGKIFFSGIDVTKFSPQQRELGLVFQNYALYPHMSVYENIAFPLYNDKH", "aa_hexdigest": "8d40a7fa5d1c53b48bb03bab9d1f50ef", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 78, "aa_cov": 0.5757575757575758, "hmm_cov": 1.0, "evalue": 2.347267753484963e-21, "score": 72.1390151977539, "start": 47, "stop": 122 } ], "seq_stats": { "molecular_weight": 14727.77869999998, "isoelectric_point": 8.66733913421631 }, "id": "AHCKFKLKGB_191", "locus": "GCA013367975_00153" }, { "type": "cds", "sequence": "contig_1", "start": 87395, "stop": 88369, "strand": "+", "frame": 2, "gene": "potA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG3842", "COG:E", "SO:0001217", "UniRef:UniRef50_D1J7L1", "UniRef:UniRef90_D1J7L1" ], "nt": "TTGAAAATTTTTGAAATCATTTTAAATGCTTATAAAGTTGATGAAAAAGCAATTGAAGATTTAAAGACAAAATGCTACTATTCAATTGACAACCCTAAAGAAACTAAAAAGTATTTAAATGAATTAATTTCACAATATAATGATATTATTGATGAACAAATTAACAAATTAAACTTTTATAAAACTAAAAAAACAGCCGAAGGAACCTCACTAACTAAAAATGTTTTGCAATATATTAAAAAACTAGGATTGCAACGCCAAGCAGAAATTTCGGAAGCTAGAGAAAAATTTTTGGGTGAAAAACAAAAACTTGATAGAGAATTTGAATTTACTCATGACAAAAATATTTTGAAAAATATATCAGCATTAAAATCGGAATTTTCAGATACTGTTTCTAAAATTAAATCTAAAAGTGCAGAAGAGATTGCTAATGCTAAAAAAGAATATTCTGAAAAATATAACGAAGCGTTAGCTCGTAATACTCAAGCAATTAAAGATGCAAAACTAGAACTAATGGAAAAGAAACGTGAACAAGCAGAATCTCCTTTAGTTAATAAAATAAAAGAAATAAAGAATAGATACGTTTTAGTTTCAAAAATAACTAATGTTGAATATACAGAAGCAGTTGAAAATGTTTTTGGTTCGTTGTTCAATTCTCAAAATATAACAAAAGGTAATTTTGATACTAAACTTAAGGCTTTAATATTTGCTTTAGATCAAGAAACTCAAAATCAAATTTCAGAAATTTCTAAAGATGTTTTGACAATTGACGAAGCAATCGTAAAAGATGTATTAGATGTAGCACAAAGAGTTGAAATTACAAAGAACTTAGCAAAACGTCCTACTCAAATGTCTGGCGGTCAACAACAACGTGTTGCCATAGCTAGAGCCATCGTTAAAAAACCAAAAATATTATTATTGGATGAACCATTGTCAAACTTGGATGCTAAGTTGCGTATTTCAACAAGAAAGTGA", "aa": "MKIFEIILNAYKVDEKAIEDLKTKCYYSIDNPKETKKYLNELISQYNDIIDEQINKLNFYKTKKTAEGTSLTKNVLQYIKKLGLQRQAEISEAREKFLGEKQKLDREFEFTHDKNILKNISALKSEFSDTVSKIKSKSAEEIANAKKEYSEKYNEALARNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRYVLVSKITNVEYTEAVENVFGSLFNSQNITKGNFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRK", "aa_hexdigest": "d2dd8516b99c826973945b47670df2db", "start_type": "TTG", "rbs_motif": null, "genes": [ "potA" ], "truncated": "both", "pseudogene": { "start": 87095, "stop": 88672, "observations": { "mutation": [ 87356, 88367 ], "stop": [ 87356, 88367 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L1", "query_cov": 1.0, "subject_cov": 0.500772797527048, "identity": 0.991, "score": 511.0, "evalue": 2.77e-176, "gene_start": 1, "gene_end": 324, "reference_start": 146, "reference_end": 469, "reference_sequence": "MKETKSREELLKDIHSFSKQRHEIKKTLPAIEIKNLVIDFGESLAVDNASFCINKGELVTLLGPSGSGKTTTLNAIAGLLRPTGGKIFFSGIDVTKFSPQQRELGLVFQNYALYPHMSVYENIAFPLYNDKHWREETLEKTKLSQLKIFEIILNAYKVDEKAIEDLKTKCYYSIDNPKETKKYLNELISQYNDIIDEQINKLNFYKTKKTAEGTSLTKNVLQYIKKLGLQRQAEISEAREKFLGEKQKLDREFEFTHDKNILKNISVLKSEFSDTVSKIKSKSAEEIANVKKEYSEKYNEALARNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRYVLVSKITNVEYTEAVENVFGSLFNSQNITKGNFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRKWIRSIQQELGITTVFVTHDQEEAMSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKSSIDVGIRGESLIEEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV" }, "paralog": false, "identity": 0.9904761904761905, "score": 917.0, "evalue": 0.0, "description": "Internal stop codon at 87356, 88367. Nonsense mutation around 87356, 88367.", "psc": { "uniref90_id": "UniRef90_D1J7L1", "gene": "PotA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_D1J7L1", "cog_id": "COG3842", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L1", "UniRef:UniRef50_D1J7L1", "COG:COG3842", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7L1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L1" ], "product": "ABC transporter ATP-binding protein" } }, "id": "AHCKFKLKGB_192", "locus": "GCA013367975_00154" }, { "type": "cds", "sequence": "contig_1", "start": 88436, "stop": 88900, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGF6", "UniRef:UniRef90_UPI00215C93E5" ], "nt": "ATGTCAATTAGCGATAAAATCGTATGTATGTCAACTGCTAAAATTCAACAAATTGGTTCTCCTATGGAACTATATTTGAAACCTAAAAATGAATTTGTTGCCAAATTCTTAGGTATGCCAGAAATGACAATATTTGAAGCGCAAATTAAAGACGGATATATAATTTACAACAATAAAAAACTACTTAAAGCTCCTGAAGGATATCAAAAAGCTTCTATTGATGTTGGTATAAGAGGCGAATCTCTAATAGAAGAAGAAGAAGGAATATTTGAAGGAAGCATAAAATTAGTTGAACATTTAGGTAAGGAAATTCAAGCTCAAATATTTATCAAAGAATTAAATAAAATTGCAAACGTTTTCTTAGCTAAAAAACACAATTATGAATTAGGTGAAGTTGTTAAGTTAAATGCAAAAAGTGAAGGCTTATTCCATTTATTTGATGTAGACAGCAAAGAACGTGTATAA", "aa": "MSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKASIDVGIRGESLIEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV", "aa_hexdigest": "cc028970f521777878587e463934f672", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 88136, "stop": 88900, "observations": { "mutation": [ 88367 ], "stop": [ 88367 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215C93E5", "query_cov": 1.0, "subject_cov": 0.42896935933147634, "identity": 0.9940000000000001, "score": 298.0, "evalue": 3.84e-99, "gene_start": 1, "gene_end": 154, "reference_start": 206, "reference_end": 359, "reference_sequence": "ANAKKEYSEKYNEALDRNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRCVLVSKITNVEYTEAVENVFGSLFNSQNITKGSFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRKWIRSIQQELGITTVFVTHDQEEAMSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKSSIDVGIRGESLIEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV" }, "paralog": false, "identity": 0.9921259842519685, "score": 482.0, "evalue": 3.17e-174, "description": "Internal stop codon at 88367. Nonsense mutation around 88367.", "psc": { "uniref90_id": "UniRef90_UPI00215C93E5", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3G8LGF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215C93E5", "UniRef:UniRef50_A0A3G8LGF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LGF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGF6" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "AHCKFKLKGB_193", "locus": "GCA013367975_00155" }, { "type": "cds", "sequence": "contig_1", "start": 88999, "stop": 89274, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCATCATTAATTACATTAACTTTATTTACTATAATTCCATTTATTTTGGTTTTAATCTTTTCATCAACATACCAAGTTGGTGCGACGGTTTATGACAAGGGATTTGGATTAAAAAACTATATTAAATTGTCAAAAGATCCAATATTCCACATTGCTATTAGAAATACATTGGTTTATGCTATATTAGCTTTACCAATTAGCTTGGCGATTTCCATTTTAATTTCATCTGCAATAAGCTTTGTAATCAAACAAAGTTTCCGTAGATTTTGA", "aa": "MLPSLITLTLFTIIPFILVLIFSSTYQVGATVYDKGFGLKNYIKLSKDPIFHIAIRNTLVYAILALPISLAISILISSAISFVIKQSFRRF", "aa_hexdigest": "960c2ff5d57665f278c0ba6e525ce04a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10181.19469999999, "isoelectric_point": 10.08996295928955 }, "id": "AHCKFKLKGB_194", "locus": "GCA013367975_00156" }, { "type": "cds", "sequence": "contig_1", "start": 89178, "stop": 89387, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTACCCGCTAGCATGTTAATAAATCCGCCTGATGTTCTAAATAAGAATGCAAAGGCGATTGAAACAGCAATTCCTGATGTTACATATGGTAGAAAGAATACTGTTTGTCAAAATCTACGGAAACTTTGTTTGATTACAAAGCTTATTGCAGATGAAATTAAAATGGAAATCGCCAAGCTAATTGGTAAAGCTAATATAGCATAA", "aa": "MFLPASMLINPPDVLNKNAKAIETAIPDVTYGRKNTVCQNLRKLCLITKLIADEIKMEIAKLIGKANIA", "aa_hexdigest": "9aa49c4a416e4a28510bc1101834c47c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7595.086599999995, "isoelectric_point": 9.342967796325684 }, "id": "AHCKFKLKGB_195", "locus": "GCA013367975_00157" }, { "type": "cds", "sequence": "contig_1", "start": 89497, "stop": 89724, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATCAGTTAACCCTGATTTATATAAATCAGCTTCTATTGATGGAAGTTCAAATTTAAAACAATTTTTTGCAATTACTTTGCCATCAATTAGAAAAACAATTACTTTCCTATTTACAGTAGGAATTATTGGATCAATTAAAACATTCCCATTGGCTTTATTTGATAACAAACCTGCTGAAGCAGCTGCAAACTCTGGTATGAGTATTATGTTATACATATTCTGA", "aa": "MLSVNPDLYKSASIDGSSNLKQFFAITLPSIRKTITFLFTVGIIGSIKTFPLALFDNKPAEAAANSGMSIMLYIF", "aa_hexdigest": "50ec91ad7d2108c5968bc822baf7c8d8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8149.496999999999, "isoelectric_point": 9.22402362823486 }, "id": "AHCKFKLKGB_196", "locus": "GCA013367975_00158" }, { "type": "cds", "sequence": "contig_1", "start": 90041, "stop": 90277, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAATGTCATTTGTAGAAAAGCAATTAAGTACAGATTCAAGAACCACAATACTATTTCCAAGAGCAATAGTAGAAGGTGGAAAGAGCTTCTTCCCTGAAAACTATAGAGATGCTTTTGAATCAGGATTCCTTGAAGCACTAGTATTTACTGCTGCTATCACAACTTTCTCGGTATTAATTAGATTAGGATTTTCAATTACATTGGGATATGCACTATCATTAAGAAACTGA", "aa": "MIEMSFVEKQLSTDSRTTILFPRAIVEGGKSFFPENYRDAFESGFLEALVFTAAITTFSVLIRLGFSITLGYALSLRN", "aa_hexdigest": "02686dad6bedcfa50cfdf51c79c06a03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8733.972199999993, "isoelectric_point": 5.221082115173339 }, "id": "AHCKFKLKGB_197", "locus": "GCA013367975_00159" }, { "type": "cds", "sequence": "contig_1", "start": 90413, "stop": 90568, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTGCCGCTTCAATATTCTTTGGATATATGTATAAAAATGCATTTGAAGCAATCCCAAACAGTGTTAAAGAATCATCAATGCTAGATGGCGCAAATGGAATCAAATACTTCTTTAAAATAGCAATGCCAATGGTTTCAGCAACAACCTGA", "aa": "MPFAASIFFGYMYKNAFEAIPNSVKESSMLDGANGIKYFFKIAMPMVSATT", "aa_hexdigest": "3fdccda69aa0966f0fbf3273c97ae301", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5636.5592, "isoelectric_point": 8.063657188415526 }, "id": "AHCKFKLKGB_198", "locus": "GCA013367975_00160" }, { "type": "cds", "sequence": "contig_1", "start": 90801, "stop": 91025, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0006401D33", "UniRef:UniRef100_A0A454C920", "UniRef:UniRef50_A0A454C920", "UniRef:UniRef90_A0A454C920" ], "nt": "ATGCAATTTCAAGACAAGGAAATGCAACAAAAGGATAGAATTATGGAAAAAGACATTAATGTTCAATTAGTAAAATTATCGAAATATCAAAAGAAAAGTAAAATTAAAAAAGTATTTATTTATATTGGTATAGGATTTGTATTAATTTCTTTGATTATTATAATGATATTGGGTGTATATGGCATAATTGGTGGTCTAGACGGATTAATAAAAAAAGGTTTATAA", "aa": "MQFQDKEMQQKDRIMEKDINVQLVKLSKYQKKSKIKKVFIYIGIGFVLISLIIIMILGVYGIIGGLDGLIKKGL", "aa_hexdigest": "8e8c01ea1f6a2a8601616c64e21b874f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0006401D33", "uniref100_id": "UniRef100_A0A454C920", "db_xrefs": [ "SO:0001217", "UniParc:UPI0006401D33", "UniRef:UniRef100_A0A454C920" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C920", "uniref90_id": "UniRef90_A0A454C920" }, "psc": { "uniref90_id": "UniRef90_A0A454C920", "uniref50_id": "UniRef50_A0A454C920" }, "pscc": { "uniref50_id": "UniRef50_A0A454C920", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C920" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8472.313499999993, "isoelectric_point": 9.823321723937987 }, "id": "AHCKFKLKGB_199", "locus": "GCA013367975_00161" }, { "type": "cds", "sequence": "contig_1", "start": 91130, "stop": 91285, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCTATGGAACTAAAAATTTATTAATATTTAAGAATAATTTAATTCAAAATAAACAAAATTTAAAATCTTTTGCACTTAAATTAAGCTTTTATGTTTTAAAACAAATGCCACAAATTAAAAGTGTTATATTGGCATATGACAATGAATATTGA", "aa": "MAYGTKNLLIFKNNLIQNKQNLKSFALKLSFYVLKQMPQIKSVILAYDNEY", "aa_hexdigest": "35315d506ca26165cd48c6380d7df148", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5999.050600000001, "isoelectric_point": 9.757241630554198 }, "id": "AHCKFKLKGB_200", "locus": "GCA013367975_00162" }, { "type": "cds", "sequence": "contig_1", "start": 91292, "stop": 92578, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L4", "UniRef:UniRef90_D1J7L4" ], "nt": "ATGAGTCAATACGTAACTTATTATTTAAAATCTAGGGGTATAAATGTTTATTTTTTACATAAGAATCATTTAGGAGTTCCTTTTTCATTCTTGGAATTTGCTTTTAAAAAATTAAATGCCTTGGATTTGGCAATATATTTTTCTTTTTTTGACAGTAAATCATGTAAAATTCAAACTATTTTTAAGAATAATCAAGAGATAAATTTTTTTGATTTTAGTAATTTTAACCTCAACGAAAATGAATACCAATTTTTAAATGATGAGCCAATATATTTAGATACTAAGATGCTGCTTAATGATTATTTAAACCAAATTACTTCAAATAGAGTAACTAAATATCATAATTCAATCCTTAATTTAATAATTAACAAATATCAATATAATTTTGAATTAATTAAGAAGATTATGGGTTTTGATGATTTGGGATATAAATTTATTAATAAACAAAGATTCACTAATGATACAATTTTTAGAGTTTCTAAAACTTTTTTAACTAGGGGCATGCAATTTAATTATTTTGCTAGATTTTTTATAAATCAAGAAGTTAAATATTATTTAGCATATAAAAAATTATTTAATTATGATTTTAAGCAAATTTTAAATGAACATTTAGTTGCATTATATATAAAATTTCAAAACACATTCTTTAGCTCAATGCTAATAAATGATAATTTTTTTAAAATAAATAAAGTTTATATAAATCAACTTTATCCCACTCCTATTTTAATAAAAGTTTGTGAAATGTTTGACATAGAATATAGTTTTGTTTTGCAACAACAAGACATTATTTTAAAAGACAATGAAAACGTTTTGTATGTTGATAATAATGATATTTCATTTTTAAGTGCTGAGTATAATTACAGAGATTCATTTAAGACATTTTTTTATATTAATGAAATGTTAAACTATTTTGAAACTCAGCAAATTGATATTTTTGACATTGAAAAAGAATTGAAATTAAAATTCTTAACCTATAAAGAATTTGAAATTGAATGTAGTATTGCAAATTTAGTTCATTTTAAAGATAAATTAAGTATTCAAAAATCAATATCTCGTATTGAAATTGATCAATTAACAGTTTTTGAAAACAAACAAGACAAATATATAAAACATATTGCTAAGATAACGTTAAAAACCAATCCTCAATGTTTTATATCTATTAGTTATAATGAAATCTCCTGCAAGTTGAAATTTTCTGTTTTTTATTCGCAAACTACAAAAATTTTCAATAAAATCAAAGAATATTTTAATAAATTTCTTTTATTAAACAAAAAAATTCGCAACTAA", "aa": "MSQYVTYYLKSRGINVYFLHKNHLGVPFSFLEFAFKKLNALDLAIYFSFFDSKSCKIQTIFKNNQEINFFDFSNFNLNENEYQFLNDEPIYLDTKMLLNDYLNQITSNRVTKYHNSILNLIINKYQYNFELIKKIMGFDDLGYKFINKQRFTNDTIFRVSKTFLTRGMQFNYFARFFINQEVKYYLAYKKLFNYDFKQILNEHLVALYIKFQNTFFSSMLINDNFFKINKVYINQLYPTPILIKVCEMFDIEYSFVLQQQDIILKDNENVLYVDNNDISFLSAEYNYRDSFKTFFYINEMLNYFETQQIDIFDIEKELKLKFLTYKEFEIECSIANLVHFKDKLSIQKSISRIEIDQLTVFENKQDKYIKHIAKITLKTNPQCFISISYNEISCKLKFSVFYSQTTKIFNKIKEYFNKFLLLNKKIRN", "aa_hexdigest": "1f57b4f9ff1ae91b128a19c7bd5cd211", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7L4", "query_cov": 1.0, "subject_cov": 0.8879668049792531, "identity": 0.97, "score": 820.0, "evalue": 6.33e-299, "valid": true, "uniref50_id": "UniRef50_D1J7L4" }, "pscc": { "uniref50_id": "UniRef50_D1J7L4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 51899.26579999998, "isoelectric_point": 8.913801765441896 }, "id": "AHCKFKLKGB_201", "locus": "GCA013367975_00163" }, { "type": "cds", "sequence": "contig_1", "start": 92594, "stop": 92977, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACACAACCTTTAAATAATTTAAAATTTTATAAAGATAGTAGTGGTAAAAATGTTCAAGCATTCCCCGATGGTACAAAAGCAATAGATGCTTTTAATATCTTTAGTGGTTATATTTCTCCTACAAAAAAATATCTAAGCCAAGATGCAAAGAGCGTAACAAAAAATATTGCCGAAGAAATAGCATTCACAAACTTTAAACATTTAATCAATGAAGGATCAATTTACAAACCTGCCGATGATGTTGCAACTAGAAGAAGTGGTCAATTAAGAGATGCAATAAAGCAAGCAGCTAGATCAATATTGAGTGATGCCAATGTTTTAAAAATTTTTGACTACGATGAATTTATTAATAAAATTAAGCTTTTGTTCCAAGAATAA", "aa": "MYTQPLNNLKFYKDSSGKNVQAFPDGTKAIDAFNIFSGYISPTKKYLSQDAKSVTKNIAEEIAFTNFKHLINEGSIYKPADDVATRRSGQLRDAIKQAARSILSDANVLKIFDYDEFINKIKLLFQE", "aa_hexdigest": "d02d4015daf3208febf247642f742650", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14378.133699999975, "isoelectric_point": 9.047122383117674 }, "id": "AHCKFKLKGB_202", "locus": "GCA013367975_00164" }, { "type": "cds", "sequence": "contig_1", "start": 92978, "stop": 93145, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGCTAATAATTTTATTAACAAAGAAGATGTAGATATAATATCACCCCCTTATTCATATTCAAAAGATGAAAAATATAGAGGAATTATTCTCCAAGGTCCCTCTGTAATCCCGGTTCATGCAAATAAAAGAGAAGATAAAGCAACTAAACTATTTATTAATTGA", "aa": "MNANNFINKEDVDIISPPYSYSKDEKYRGIILQGPSVIPVHANKREDKATKLFIN", "aa_hexdigest": "a636f015c8c28daf7d0672cc05bee5c7", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6293.081000000002, "isoelectric_point": 8.029553413391113 }, "id": "AHCKFKLKGB_203", "locus": "GCA013367975_00165" }, { "type": "cds", "sequence": "contig_1", "start": 93476, "stop": 93628, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTACTTCAACAGCTGCATATCATTACACTTTTGTTAGTGAAAATGGAAAAAATAACAATTATACAATCAAGCAGGCAAACAGAGTGTTAAATCCAAACACAACAATTGAAATATTAAACGATACTCCCGAAAATAATGAAAAATGA", "aa": "MVITSTAAYHYTFVSENGKNNNYTIKQANRVLNPNTTIEILNDTPENNEK", "aa_hexdigest": "a0331a5f20af297babdb361e71af83ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5687.181100000001, "isoelectric_point": 5.573768043518065 }, "id": "AHCKFKLKGB_204", "locus": "GCA013367975_00166" }, { "type": "cds", "sequence": "contig_1", "start": 93683, "stop": 93994, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAATTATTTGACAACTATAGCGATTTAATTAATTTTGCAATTCAAGCAAAAAGATTATACAACGCGGACCCTACATTAAACGTTTTGGGAATAGATGAAATACCTTCGGCTATCAATGTTATGGCAACATCAATCATTGGTAGTGATATCAATAAAAACTATATAAACCCAGATGATAATTGCCTAACAACTGGTGGCTACAATTATGACAATTTTATTGAAAATCCTGATAGCCCACAAGCGAAATTATTTAAAAAAGTTTCTTCTATTATTTTAACAGGTATTCAAGAAGGTGCCGTTTGA", "aa": "MSDKLFDNYSDLINFAIQAKRLYNADPTLNVLGIDEIPSAINVMATSIIGSDINKNYINPDDNCLTTGGYNYDNFIENPDSPQAKLFKKVSSIILTGIQEGAV", "aa_hexdigest": "49cbf38464efebd4410f742245240b09", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11305.56629999999, "isoelectric_point": 4.232879447937012 }, "id": "AHCKFKLKGB_205", "locus": "GCA013367975_00167" }, { "type": "cds", "sequence": "contig_1", "start": 94064, "stop": 94219, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTACAGTAGCTAAAGCATTAGTTGGAAAATTCGTTAAAGAATTAATGGAATTAGGAGTTAAATTAGATAGTCAAAATAGCAGCAAATTGCAATCTTATTTAGACTATTACAATAATAATAAACAAGAAGCAAAAGGCGTTGATGGTATTTGA", "aa": "MTTVAKALVGKFVKELMELGVKLDSQNSSKLQSYLDYYNNNKQEAKGVDGI", "aa_hexdigest": "20af473d2d9f309b8a852887fb0a98c5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5667.423100000003, "isoelectric_point": 7.983522987365723 }, "id": "AHCKFKLKGB_206", "locus": "GCA013367975_00168" }, { "type": "cds", "sequence": "contig_1", "start": 94247, "stop": 94351, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTAGCAATTGATGACAAAGATTTTAAAAAATACAACATTGAACCAGTATTTAATGTTGCAAACTCTTTAATTGCAAATAATAAAGATAACAAAAAATGA", "aa": "MNLAIDDKDFKKYNIEPVFNVANSLIANNKDNKK", "aa_hexdigest": "80613b8fafd2806a7d39ba00a840f1b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3925.4239000000007, "isoelectric_point": 8.063786125183103 }, "id": "AHCKFKLKGB_207", "locus": "GCA013367975_00169" }, { "type": "cds", "sequence": "contig_1", "start": 94526, "stop": 94735, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGACTTGGCAAATTATTCACTTTAATGACTATAGGTTTACTTCCTACGACTACATTGATTTTATCCTCATGTTCAACAACAGAAAATTCGAAATACGGATTTGATCAAGAAGATGATGGGCAATTAGTATTAGCAATGGGATTTTTAAAAAACGATTGGCAAGGGCTTGCTATTCAAGGGATTGTTGATGCATACAACAAATGA", "aa": "MKRLGKLFTLMTIGLLPTTTLILSSCSTTENSKYGFDQEDDGQLVLAMGFLKNDWQGLAIQGIVDAYNK", "aa_hexdigest": "2ddd18768533f7aaaa46258b0a3dc368", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7615.7312, "isoelectric_point": 4.9838361740112305 }, "id": "AHCKFKLKGB_208", "locus": "GCA013367975_00170" }, { "type": "cds", "sequence": "contig_1", "start": 94995, "stop": 95387, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACACAACCATTGAATAATTTAGAGCTTTATAAAGTTGATAGCAATGGCAAAAAAACAAACATTCAAAAATTCCCTGATGGTACAAAAGCAATAGATGCTTTCAATGCCTTTGGTGGATATATTTCGCCTACAAATAAATATCTAAGTCAAGATGCAAAGAGTGCAACAAAAAATATTGCTGATGAAATAGCATTCACAAACTTTAAACATTTAATTAATGAAGGATCAATATACAAACCCGCTGATGATATTGCAACTAGAAAAGGCGATCAATTAAGGGATGCAATAAAGCAAGCAGCTAGATCAATATTGAGTGATGCCAATGTTTTAAAAGTTTTTGACTACAAAGATTTTATTAATAAAATTAAGCTATTGTTCCAAGAATAA", "aa": "MYTQPLNNLELYKVDSNGKKTNIQKFPDGTKAIDAFNAFGGYISPTNKYLSQDAKSATKNIADEIAFTNFKHLINEGSIYKPADDIATRKGDQLRDAIKQAARSILSDANVLKVFDYKDFINKIKLLFQE", "aa_hexdigest": "14657af385a140e6505471275e2336d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14642.412599999976, "isoelectric_point": 9.001930046081544 }, "id": "AHCKFKLKGB_209", "locus": "GCA013367975_00171" }, { "type": "cds", "sequence": "contig_1", "start": 95886, "stop": 96044, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATAAATATTGGTTCTACTGCTGGATATCGTCACACATTCGTTGGCAATAATGAAAAAAATAGTACTTATATAATTAAACAAGCCAACAGGGTATTAGATCCAACAACAACAATTGAAATATTAAATAATACTCCTAAAAATAATGAAAAATGA", "aa": "MAINIGSTAGYRHTFVGNNEKNSTYIIKQANRVLDPTTTIEILNNTPKNNEK", "aa_hexdigest": "9f125fe2d5c33ffda7d748534f0afbda", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5806.436100000001, "isoelectric_point": 9.301836967468262 }, "id": "AHCKFKLKGB_210", "locus": "GCA013367975_00172" }, { "type": "cds", "sequence": "contig_1", "start": 96093, "stop": 96404, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAATTATTTGACAACTATAGCGATTTAATTAACTTTGCAATTCAAGCAAAAAGATTATATAATGTTGACCCTACATTAAATGTTTTAGGAATAGATGAAATACCTTCAGCTATCAATGTTATGGCAACATCAATCATTGGTAGTGATATCAATAAAAACTATATAAACCCAGATGATAATTGCCCAGCAACTGGTGGCTACAATTATGACAATTTTATTGAAAATCCTAATAGCCCACAAGCGAAATTATTTAAAAAAGTTTCTTCTATTATTTTAAGAGGTATTCAAGAAAATGCTGTTTGA", "aa": "MSDKLFDNYSDLINFAIQAKRLYNVDPTLNVLGIDEIPSAINVMATSIIGSDINKNYINPDDNCPATGGYNYDNFIENPNSPQAKLFKKVSSIILRGIQENAV", "aa_hexdigest": "5d05e34f3adce510e9e3e45a56e58f0a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11398.699299999987, "isoelectric_point": 4.478082847595215 }, "id": "AHCKFKLKGB_211", "locus": "GCA013367975_00173" }, { "type": "cds", "sequence": "contig_1", "start": 96474, "stop": 96581, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTAGGAGTTAAATTAGATAGTCAAAATAGCAGCAAATTGCAATCTTATTTAGACTATTACAATAATAATAAACAAGAAGCAGAAGGCGTTGATAATATTTGA", "aa": "MALGVKLDSQNSSKLQSYLDYYNNNKQEAEGVDNI", "aa_hexdigest": "2b31644653e7e18f194895bf18a67834", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3950.2562000000016, "isoelectric_point": 4.436874580383301 }, "id": "AHCKFKLKGB_212", "locus": "GCA013367975_00174" }, { "type": "cds", "sequence": "contig_1", "start": 96657, "stop": 96761, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTAGCAGTTAATGATGAAGATTTTAAAAAATATAACATTGAACCTGTATTTAATAATGCAAACTCTTTAATTGCAAATAATAAAGATAACAAAAAATGA", "aa": "MNLAVNDEDFKKYNIEPVFNNANSLIANNKDNKK", "aa_hexdigest": "c779ef403daf47c8605ad34b0f99d431", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3926.3258000000014, "isoelectric_point": 6.000344657897949 }, "id": "AHCKFKLKGB_213", "locus": "GCA013367975_00175" }, { "type": "cds", "sequence": "contig_1", "start": 96948, "stop": 97157, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTACCAAATTATTTACAGCAGTAGCGGCAGGATTATTTCCCGCAACTGCATTAATTTCAGCCTCGTGCTCAACAACAGAAAATTCAAAATATGGATTTGACCAAGATGATGACGGACAATTAGTATTAGCAACAGGATTTTCAAAAAGTAACTGGCAAGGATTTGCCATTCAAGGAATTGTTAATACATATAATAATTGA", "aa": "MKKITKLFTAVAAGLFPATALISASCSTTENSKYGFDQDDDGQLVLATGFSKSNWQGFAIQGIVNTYNN", "aa_hexdigest": "9ae150dd452904b40b63e5b2c8ed22e4", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7368.1831, "isoelectric_point": 5.952713584899903 }, "id": "AHCKFKLKGB_214", "locus": "GCA013367975_00176" }, { "type": "cds", "sequence": "contig_1", "start": 97568, "stop": 98062, "strand": "+", "frame": 2, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "SO:0001217", "UniRef:UniRef50_Q9PPZ2", "UniRef:UniRef90_D1J7L7" ], "nt": "ATGTCAAAATTTGTTGGCTCAATGTTTAAAAAGAGCCGTAGATATGGAATTTCTTTATTAGAAAATAATAAAGAATTCACAAAAGGCAAAAAACGTACAACTGCCCCTGGACAACACGGAGCAAAAAGAAGCAAACCATCAGACTATCAACTACACATGTATGAAAAACAAAAAGTTAGATATATGTATGGTTTAAATGAACGTCAATTTAAAAATTTATTTATTAAAGTTTCTAAAAAAGCCGGAGTTACTGGTATTAACTTACTACAAACTGTTGAATCAAGATTAGACAATCTAGTATTTAGAGCAGGATTTGCAAGAACAAGAGCTCAAGCAAGACAATTAGTAAATCATGGACACTTCACAGTTGATGGATGCAAAGCAAACATTCCTTCAATGATTGTTAAAGTAGGTTCAGTTATTGAACTAAAAGAAGCATTACAAAATAATGTTGCTATTAAAGATGCTATTGAAGTAATGACAGTTGCTCCATGA", "aa": "MSKFVGSMFKKSRRYGISLLENNKEFTKGKKRTTAPGQHGAKRSKPSDYQLHMYEKQKVRYMYGLNERQFKNLFIKVSKKAGVTGINLLQTVESRLDNLVFRAGFARTRAQARQLVNHGHFTVDGCKANIPSMIVKVGSVIELKEALQNNVAIKDAIEVMTVAP", "aa_hexdigest": "da01c9dae17b2a1d09586afb5cf50a64", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7L7", "query_cov": 1.0, "subject_cov": 0.8241206030150754, "identity": 1.0, "score": 317.0, "evalue": 4.69e-109, "valid": true, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_Q9PPZ2", "cog_id": "COG0522", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "AHCKFKLKGB_215", "locus": "GCA013367975_00177" }, { "type": "cds", "sequence": "contig_1", "start": 98206, "stop": 98517, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63", "UniRef:UniRef90_UPI000AFE6B63" ], "nt": "TTGTTTACTTTTGTAATTATATTAATATATCTAATATCACTTATTTTGGGTATTCTTTTTTATCAAATTAGTCATGATAGACTCACAAACTATTTGATTATTGTTTTAAGTTCTATAGTTTTCCCTGAAATTTTAAATACGCTGTTTTCACTTTCAAATATAACAATGACGCTTAATCCTTTATGCAATAATATTTATCTAATTAATTTAGTTTATATACCAGCATTTATCTTATTTATTGTTTTAATTACAAAAACAAAAGAAAAACAATTTATATTTTTTAAAAAAGTTAATTATTTAAAAAATAAATAA", "aa": "MFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNPLCNNIYLINLVYIPAFILFIVLITKTKEKQFIFFKKVNYLKNK", "aa_hexdigest": "2542b514f7902e4487960cf43ae8ee7b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 98206, "stop": 98703, "observations": { "mutation": [ 98574, 98655 ], "stop": [ 98574, 98655 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AFE6B63", "query_cov": 1.0, "subject_cov": 0.6242424242424243, "identity": 0.971, "score": 176.0, "evalue": 1.42e-54, "gene_start": 1, "gene_end": 103, "reference_start": 63, "reference_end": 165, "reference_sequence": "MILFINQSYYRIKDGRDHSKTPVYLENTKIIEYFYVYIFRLTYWFLLSFLLIKITNNFKKAILFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNSLCNNIYLINLVYIPAFILFIVLITKTKEKQFIFFKKVNYVKNK" }, "paralog": false, "identity": 0.9151515151515152, "score": 291.0, "evalue": 1.79e-102, "description": "Internal stop codon at 98574, 98655. Nonsense mutation around 98574, 98655.", "psc": { "uniref90_id": "UniRef90_UPI000AFE6B63", "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AFE6B63", "UniRef:UniRef50_UPI000AFE6B63" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12112.638799999979, "isoelectric_point": 9.519675636291506 }, "id": "AHCKFKLKGB_216", "locus": "GCA013367975_00178" }, { "type": "cds", "sequence": "contig_1", "start": 98653, "stop": 99003, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q2Z3", "UniRef:UniRef90_D1J7L8" ], "nt": "TTGCTAGCAGGATTTGTAATTTATCTTTTAATATCAGCAATAATATTAAAAATAGATGGTCAAATCGATCAATTTAATCTATTTCCTTTAATAAATCAAATATTTTTAATCGCTCCTTCTTATACTACCCAGCTTCTTTTAACATTATTATCAATAGGACTATTTTTAAACTTCTATTTTGTTAAACGATATCTATCAAAACGAAGTATAGAACTAGATCACAAAGAAAAAAGAAGAAACTATTTAATAATAATATTGACGACTGCAATATCATTGATTGTAATATTTTTAATTAACGTAATGATTTTGTTTATTAAACAAAATTTTTATGAAATCGCCTACAAACATTGA", "aa": "MLAGFVIYLLISAIILKIDGQIDQFNLFPLINQIFLIAPSYTTQLLLTLLSIGLFLNFYFVKRYLSKRSIELDHKEKRRNYLIIILTTAISLIVIFLINVMILFIKQNFYEIAYKH", "aa_hexdigest": "c73a697da9cbff9d06b635528a5bf502", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 98350, "stop": 99067, "observations": { "insertion": [ 99046 ], "stop": [ 98574, 98655, 99009 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L8", "query_cov": 1.0, "subject_cov": 0.40559440559440557, "identity": 0.94, "score": 198.0, "evalue": 1.09e-61, "gene_start": 1, "gene_end": 116, "reference_start": 22, "reference_end": 137, "reference_sequence": "MEKFVNEKNKKQLLLFLPYWILLAGFVIYLLVSAIMLKIDGQIDQFNLFPLINQIFLIAPSYTTQLLLTLLSIGLFLNFYFVKRYLSKRSIELDHKEKRRNYLIIILTTAISLIVIFLINVMILFIKQNFYKIVSGHWNTKTPVYLENTKIIEYFYVYIFRLTYWFLLSFLLIKITNNFKKAILFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNSLCNNIYLINLVYIPAFILFIVLITKIKEKQFIFFKKVNYAKNK" }, "paralog": false, "identity": 0.9621848739495799, "score": 429.0, "evalue": 5.29e-155, "description": "Internal stop codon at 98574, 98655, 99009. Frameshift due to insertion around 99046.", "psc": { "uniref90_id": "UniRef90_D1J7L8", "uniref50_id": "UniRef50_A0A6A8Q2Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L8", "UniRef:UniRef50_A0A6A8Q2Z3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q2Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q2Z3" ], "product": "ABC transporter permease" } }, "id": "AHCKFKLKGB_217", "locus": "GCA013367975_00179" }, { "type": "cds", "sequence": "contig_1", "start": 99235, "stop": 99801, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L9", "UniRef:UniRef90_D1J7L9" ], "nt": "ATGATTTGTTCTAGATTACTATTGCCTTTGAATGATGTTGATGAATATAAATTAATTCCGCTTGTTAGAACAATCGAATTTACAATTTATACAAAGGCTTCAAAAATTAAACACGATAATGAAGTACTTCTAACTTCAATATCAAATAATTTAAGTCAATATGACATTGATAATTTTCAAGGTTTATATTGCGATATAAACCAAGCATTTGTAGCTGATAATCAATTATTTGACGAAGAAACAGAGTACCAATTTAAATTCAGCAATTCAAATGATGAAGATAATTACCAAGCAAGTTATATTATTCAAAAACTAATAAAAAAACTATTAAATTTTGTCAATGATGAAGACCTTAATTATTGCTTTATTATTATGACAAAAATCCAAAATAATATTATTAAACCCTTTTATATATATTGCAATCCAGAAGATGCAAAAAAGGAACTTGAACAACTATTTAAAACATTAGATAATACAAAATACGAAGCATTACTATTAGAAGCAACAAATACTTTTAGTTTTGAATTAAAAAAATTTAATGAGGAATATTTAAACAAATCTAGTTGA", "aa": "MICSRLLLPLNDVDEYKLIPLVRTIEFTIYTKASKIKHDNEVLLTSISNNLSQYDIDNFQGLYCDINQAFVADNQLFDEETEYQFKFSNSNDEDNYQASYIIQKLIKKLLNFVNDEDLNYCFIIMTKIQNNIIKPFYIYCNPEDAKKELEQLFKTLDNTKYEALLLEATNTFSFELKKFNEEYLNKSS", "aa_hexdigest": "eea2725ffe320e4396b9a0952b9a4b20", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_D1J7L9", "query_cov": 1.0, "subject_cov": 0.8663594470046083, "identity": 0.9840000000000001, "score": 364.0, "evalue": 8.47e-127, "valid": true, "uniref50_id": "UniRef50_D1J7L9" }, "pscc": { "uniref50_id": "UniRef50_D1J7L9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22238.924600000028, "isoelectric_point": 4.583007621765137 }, "id": "AHCKFKLKGB_218", "locus": "GCA013367975_00180" }, { "type": "cds", "sequence": "contig_1", "start": 99979, "stop": 100146, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175933389.1", "SO:0001217", "UniParc:UPI000A408A13", "UniRef:UniRef100_UPI000A408A13", "UniRef:UniRef50_UPI000305A2B6", "UniRef:UniRef90_UPI000305A2B6" ], "nt": "ATGAATAACAAAAATAATAAAGTAAGTGGAAACACACATACAAGACAACAAATGAATAATTATTCAAATCAAAATAATTCTAATAATTCAGCATACAGATCGAATTTAAACAATCATTCGAATCAATTGAATCCAAACAATAGCAACTACAAGGGCAACAAAAAATAA", "aa": "MNNKNNKVSGNTHTRQQMNNYSNQNNSNNSAYRSNLNNHSNQLNPNNSNYKGNKK", "aa_hexdigest": "331729aee084b469b468fb82cb6c01f3", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000A408A13", "ncbi_nrp_id": "WP_175933389.1", "uniref100_id": "UniRef100_UPI000A408A13", "db_xrefs": [ "SO:0001217", "UniParc:UPI000A408A13", "RefSeq:WP_175933389.1", "UniRef:UniRef100_UPI000A408A13" ] }, "ips": { "uniref100_id": "UniRef100_UPI000A408A13", "uniref90_id": "UniRef90_UPI000305A2B6" }, "psc": { "uniref90_id": "UniRef90_UPI000305A2B6", "uniref50_id": "UniRef50_UPI000305A2B6" }, "pscc": { "uniref50_id": "UniRef50_UPI000305A2B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000305A2B6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6325.600600000003, "isoelectric_point": 10.461107444763183 }, "id": "AHCKFKLKGB_219", "locus": "GCA013367975_00181" }, { "type": "cds", "sequence": "contig_1", "start": 100318, "stop": 100494, "strand": "-", "frame": 1, "gene": null, "product": "putative dsRNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D72BC", "UniRef:UniRef90_UPI00215D72BC" ], "nt": "ATGTTAGATATTGAACATTTTTTTAGAGATGTTGATTGCAGAGAAAAAAGACCGGATAAATTTAAATTAGAAAATGCTATCAATACATTAAAAGAATTAGCAGAGCGTGGTGAATGTTCAATGCCAATATATCAAATATCAGAAAAACCTATAATTGATGATAACGGCAAACAATGA", "aa": "MLDIEHFFRDVDCREKRPDKFKLENAINTLKELAERGECSMPIYQISEKPIIDDNGKQ", "aa_hexdigest": "97bea04649db311e0a0f588e9852fe68", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 100156, "stop": 100584, "observations": { "mutation": [ 100278, 100317, 100320, 100530 ], "stop": [ 100278, 100317, 100320, 100530 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D72BC", "query_cov": 1.0, "subject_cov": 0.4084507042253521, "identity": 0.966, "score": 117.0, "evalue": 1.01e-32, "gene_start": 1, "gene_end": 58, "reference_start": 31, "reference_end": 88, "reference_sequence": "MKADLFEAIIGAIAIDSGWDIKRLKNIIIKMLDIERFFRDVDCREKRPNKFKLENAINTLKELAERGECSMPIYQISEKPIIDDNGKQWWHCRCNVNFRNLQWMGLWTKYTEGYGTSKKLAKKMAAYNMLCDHYGLPNEYKN" }, "paralog": false, "identity": 0.9366197183098591, "score": 271.0, "evalue": 1.27e-95, "description": "Internal stop codon at 100278, 100317, 100320, 100530. Nonsense mutation around 100278, 100317, 100320, 100530.", "psc": { "uniref90_id": "UniRef90_UPI00215D72BC", "product": "putative dsRNA-binding protein", "uniref50_id": "UniRef50_UPI00215D72BC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D72BC", "UniRef:UniRef50_UPI00215D72BC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D72BC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D72BC" ], "product": "putative dsRNA-binding protein" } }, "id": "AHCKFKLKGB_220", "locus": "GCA013367975_00182" }, { "type": "cds", "sequence": "contig_1", "start": 100528, "stop": 101004, "strand": "-", "frame": 1, "gene": null, "product": "RNase III domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1G3X3E2" ], "nt": "ATGGGAATAGAAGAATGGCAAGAATATATTTATCAAATTGAATATGCAATAGGTTATTCCTTTAAAAATAAATTTTTACTTTATCAAGCGTTTACCAGAAGTTCTTATTCAAATCAATATGGAATTGAAAATAATGAAGTTCTTGAATTTATAGGAGATTCAGTTTTGAGCTTTTGCGTAGTTCAACTAATGGCAGAAGAATTTGGCTTTTTGAAATCAGCTTCGCAATATTACAACAAATATGAAGATCTTGAAGAATATTGCCTTATAGCCAATAAAAATGAATCCGATTTTACTGATTTAAAAAATCAAATCGTAAGCAATAAAACACTAGCTAAAAAAATTAATAAATTAGGTTTTGCTAAGTATTTAGTTTTAGGAGATAATGATAAAAGAAATAATGTTGCTAATCAAGATAGTGTGAAAGCTGATTTATTTGAAGCTATAATTGGTGCAATAGCAATTGACAGTGGTTGA", "aa": "MGIEEWQEYIYQIEYAIGYSFKNKFLLYQAFTRSSYSNQYGIENNEVLEFIGDSVLSFCVVQLMAEEFGFLKSASQYYNKYEDLEEYCLIANKNESDFTDLKNQIVSNKTLAKKINKLGFAKYLVLGDNDKRNNVANQDSVKADLFEAIIGAIAIDSG", "aa_hexdigest": "2408c7c5d73392cc4b8eedf841174f3b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A2K9A1I8", "query_cov": 0.9810126582278481, "subject_cov": 0.8908045977011494, "identity": 0.535, "score": 160.0, "evalue": 2.77e-47, "valid": false, "product": "RNase III domain-containing protein", "uniref50_id": "UniRef50_A0A1G3X3E2" }, "pscc": { "uniref50_id": "UniRef50_A0A1G3X3E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1G3X3E2" ], "product": "RNase III domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_221", "locus": "GCA013367975_00183" }, { "type": "cds", "sequence": "contig_1", "start": 101193, "stop": 102188, "strand": "+", "frame": 3, "gene": null, "product": "Putative gamma-glutamylcyclotransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2K9YSY5", "UniRef:UniRef90_A0A2K9YSY5" ], "nt": "TTGAACAATTTAATTACACTTATATTAAGCTATGATTTTATTGCATTCAAGAATAAATTTAAATGTATGTATATTGATTTTGTTATTTTGGTTTTTAAAATAGAATTACAATATGAGTTTTATTTGATTTTAGCGTTTTATAATAATTTAAATAATATAATATTTATGTATTTAGGAGAAATTATGTCAAACGAAGAAAAAATTTATGTTTTTAGTTATGGAACTATTCAAGATCCACAATTTTATAAGGAATTATTACCAAACTCAAAGCCAATGCCAGCAATATTAAATGGTTATGCAAAATGTGTTGATGAAACAATGTATTTTTTATTAAAAAAAGATTTGTCAAGTCAAGTTAAGGGAAGCGTATTTGAAATTAGTAAAGAAGAATTGTTTTTAATTGATAGATGGGAATTATTTCCTCAATATCAAAGATTTCAAGTTAATGTTTTATTAACCGAAACAAATGAAATTTTAGAAAATGTTTATGTATATACAAAATTAGAAGTTGGAAAATATTATTTAGCAACAGATGATATGGGATTTTCTAGAAATCCTAACGCTAATGAAAATAATTTAAATGCTTTTATTGAAATGGAAAAGGCAATAAAAGATTTTCCATTAACTGACTATATATTTTTATATGATATAAATGAACAAGAATTTGAAGAAATTAATAAATTAACTCACCCTTATGCTGCCTTAATTATTGATGATAAGGAAAATAGAAATTATGTTGCAATTCATGGATCAATTTTTGCAATTAAAGAAGATGGCAAAATGTATGGAGCCTTAACTTCGTTTAGTCAAAAGTCAAATTTAAATAGTATTTTTTATTATCAAGCATTTAATGAAAAGTTATTAAATAGCAAACCAGAAATAACGTTAAAATCGTTATACGATAATACAAATATAGATTTTTTAATTAATAAAAAACCTGTTTATTATTTAAGCTCAAGAGAAGATAAAACAATCAATGAAACTCAAGTTGGTTGA", "aa": "MNNLITLILSYDFIAFKNKFKCMYIDFVILVFKIELQYEFYLILAFYNNLNNIIFMYLGEIMSNEEKIYVFSYGTIQDPQFYKELLPNSKPMPAILNGYAKCVDETMYFLLKKDLSSQVKGSVFEISKEELFLIDRWELFPQYQRFQVNVLLTETNEILENVYVYTKLEVGKYYLATDDMGFSRNPNANENNLNAFIEMEKAIKDFPLTDYIFLYDINEQEFEEINKLTHPYAALIIDDKENRNYVAIHGSIFAIKEDGKMYGALTSFSQKSNLNSIFYYQAFNEKLLNSKPEITLKSLYDNTNIDFLINKKPVYYLSSREDKTINETQVG", "aa_hexdigest": "12c0f05bbb1e23b4987b2cd34d02e117", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2K9YSY5", "query_cov": 0.8912386706948641, "subject_cov": 0.8858858858858859, "identity": 0.993, "score": 584.0, "evalue": 1.76e-209, "valid": true, "product": "Putative gamma-glutamylcyclotransferase", "uniref50_id": "UniRef50_A0A2K9YSY5" }, "pscc": { "uniref50_id": "UniRef50_A0A2K9YSY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2K9YSY5" ], "product": "Putative gamma-glutamylcyclotransferase" }, "genes": [], "id": "AHCKFKLKGB_222", "locus": "GCA013367975_00184" }, { "type": "cds", "sequence": "contig_1", "start": 102409, "stop": 103998, "strand": "+", "frame": 1, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "COG:COG0322", "COG:L", "GO:0006289", "GO:0009380", "GO:0009381", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_A0A2Z5IPW9", "UniRef:UniRef90_D1J7M2" ], "nt": "ATGTTGCAATACTTTGATATAAAAATGCAAAATTCTTTTAAAACAGAAAAAATGAGGCAAGAGATTGCTGATTTTTCTACAATCATTTTAAGCTCGGAACGTGAAGCATTTATTGAAGAAAGAAAATTGATTGATTTTTATAAACCATTTTACAATGTTCTTTTTCCAACTAGAGATAGTTTCCCTTATATAAGAGTTAAATTACTAAGTACTAATAAATTAATAATTGACATAAAAAATCATTATAAAAAAGAACCAAATTCTATATATTATGGTCCTATTCCCGACAACAAAAATTTCAAACCATTAATTAGATATTTGAATCATTTACTACTTTCTAAGGATGGAATCATAATTGAAAAACAAAGTCCTGAATTTGCAAAGCAGAGATTTGAACAAGCCAAATCAATCATGAAATTTGGTCCTGATTTTAAAAGAAATTTAAAACAAAAAATCAATGAGGCAATTGAAATGAACCTGTTTGAGCAGGCGAGATTTTATGACCAAATTTTAGATTTATTAAATTACAATAAACAAAGTCAAAATATTTTTATAAAAACTATCAAAGATATTGATGTCTTTGGTTTTTATCAATATCAAAACTATATGTTGGTTAATGTTATGATTTATAAAAACGGATCATTAGTAAATTCTTATGACTATATTTTTGAAATAATATTGAATGAACATGAATTTGTTAATCAATTCATTGATGAATTTTATGTTAATAATCAATTACCTGATCAAATAATAATACCTTTAAAATATTATGAATTAGGTGGATTTGATTATAACGACAAAGTAAGCGCGCCATCGAATAAATTAAATAAAAACTTAATTGATATTGCTATTGAAAATGCATCAAATAATATTGAACAAAAAATAAAAAATTATTTGAGTAAGCAAACAAAAGACAATGAAATTTTCAATAAATTATGTTCATATTTGAATGTTTACGCAGAAAGAATTGTTTTATTTGACAATTCATTTTTATCAAATACTAACGAAGTAATAGGAGCAGCTTGCTATTTTAAAAATGGCCAAGCTTTTAAAAAATTCTACCGTTTCTATAATTTAAAATTGAATTTATTAAGAAATGCAGATGTTGAATATATGCAACAAACTTCCTTTAATTATTTAAAAGATTTGGGAGATCAAGTTGATGTTGTTATTGTTGATGGTTCAATTGGTCAAATAAATGAAGTTAAAAAATCAATGGCATTTTTAAATTTAAATATTCCTCTATTCGGGCTGGTTAAAAATAGTAAACATGAAACTCAAAAATTAATTGATTCAAACAACAACGAAATTAAAATAGATGACCAAGATGTATTTAATTTGCTTTCAAGAATGCAAACAGAGGTTGATAGGTTTGCAAAATATAGATATAACCAAAAACAAAGAAAATCAATGTTATCTAATCCTCTATTAAAAATAAAAGGTATTGGATTTAAGACAATAGAAAAATTAATCAATTATTTTGGCAGTTATAAAAAAATTCAACAAGCTTCTTTTGATGAAATATCAAAAGTAGCAAACAAAAAAGTTGCTAAGCTAATTTGTGAAGACAAAGGGGAATCGTTTTATTAA", "aa": "MLQYFDIKMQNSFKTEKMRQEIADFSTIILSSEREAFIEERKLIDFYKPFYNVLFPTRDSFPYIRVKLLSTNKLIIDIKNHYKKEPNSIYYGPIPDNKNFKPLIRYLNHLLLSKDGIIIEKQSPEFAKQRFEQAKSIMKFGPDFKRNLKQKINEAIEMNLFEQARFYDQILDLLNYNKQSQNIFIKTIKDIDVFGFYQYQNYMLVNVMIYKNGSLVNSYDYIFEIILNEHEFVNQFIDEFYVNNQLPDQIIIPLKYYELGGFDYNDKVSAPSNKLNKNLIDIAIENASNNIEQKIKNYLSKQTKDNEIFNKLCSYLNVYAERIVLFDNSFLSNTNEVIGAACYFKNGQAFKKFYRFYNLKLNLLRNADVEYMQQTSFNYLKDLGDQVDVVIVDGSIGQINEVKKSMAFLNLNIPLFGLVKNSKHETQKLIDSNNNEIKIDDQDVFNLLSRMQTEVDRFAKYRYNQKQRKSMLSNPLLKIKGIGFKTIEKLINYFGSYKKIQQASFDEISKVANKKVAKLICEDKGESFY", "aa_hexdigest": "f0cada0cd66d3b0e670b3a6f02ef17ca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M2", "query_cov": 1.0, "subject_cov": 0.921602787456446, "identity": 0.991, "score": 1017.0, "evalue": 0.0, "valid": true, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_A0A2Z5IPW9", "cog_id": "COG0322", "cog_category": "L", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0006289", "GO:0009380", "GO:0009381" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z5IPW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z5IPW9" ], "product": "Excinuclease cho" }, "genes": [ "uvrC" ], "id": "AHCKFKLKGB_223", "locus": "GCA013367975_00185" }, { "type": "tRNA", "sequence": "contig_1", "start": 104063, "stop": 104138, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 76.3, "nt": "GGTCGTGTAGCTCAGGGGATAGAGTACGTGCCTTCTAAGCATGTGGTCGCAGGTTCGAATCCTGCCACGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 104096, 104098 ], "id": "AHCKFKLKGB_4", "locus": "GCA013367975_00186" }, { "type": "tRNA", "sequence": "contig_1", "start": 104208, "stop": 104281, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 71.2, "nt": "GCGAGTGTAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGAATACGTGGGTCCGATTCCCATCACTTGCACCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 104240, 104242 ], "id": "AHCKFKLKGB_5", "locus": "GCA013367975_00187" }, { "type": "tRNA", "sequence": "contig_1", "start": 104284, "stop": 104357, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 70.8, "nt": "GCGGATGTAGTTCAATGGTAGAACATCAGGTTGCCAATCTGAATACGTGGGTCCGATTCCCATCATCCGCACCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 104316, 104318 ], "id": "AHCKFKLKGB_6", "locus": "GCA013367975_00188" }, { "type": "cds", "sequence": "contig_1", "start": 104688, "stop": 105104, "strand": "+", "frame": 3, "gene": null, "product": "FMN-dependent NADH-azoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W7GCQ1" ], "nt": "ATGGCAAAAGTATTAATAATATATGGTTCAGTTATACCAAGAGAAAATTCAATTTCAACAAAAATGTTAGATCTTTTTGTTGATGAATATAAAAGAAATAATATTAATGATGAAATAAAAATTCTTGATTTAAATAATGAAGATATGTATTCATTAACTGACAAGAATTTTAAATTGTTTTATAGTGAAGAAAACTCTGACAAATATATAAATTTATTGAAGTCTGTTGATAAAGTTGTTATGAATGCGCCGATGATAAACTTCAATATTCCAACTATAATCAAGGCATTTTTTGACCGTATTGCAGTTGCAAATAAAACATTTTCATATAAATACGCAAGCAAAGGTTCTTCAATTGGTTTATTGAATAATTTAAAAGTTCAAATAATCGCAACGCAAGGAGCTCCATTAGGTTGA", "aa": "MAKVLIIYGSVIPRENSISTKMLDLFVDEYKRNNINDEIKILDLNNEDMYSLTDKNFKLFYSEENSDKYINLLKSVDKVVMNAPMINFNIPTIIKAFFDRIAVANKTFSYKYASKGSSIGLLNNLKVQIIATQGAPLG", "aa_hexdigest": "192337f9037dd954a8548cfbf08c4d5f", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A2W7GCQ1", "query_cov": 0.8478260869565217, "subject_cov": 0.9212598425196851, "identity": 0.556, "score": 120.0, "evalue": 1.34e-32, "valid": false, "product": "FMN-dependent NADH-azoreductase", "uniref50_id": "UniRef50_A0A2W7GCQ1" }, "pscc": { "uniref50_id": "UniRef50_A0A2W7GCQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W7GCQ1" ], "product": "FMN-dependent NADH-azoreductase" }, "genes": [], "id": "AHCKFKLKGB_224", "locus": "GCA013367975_00189" }, { "type": "cds", "sequence": "contig_1", "start": 105384, "stop": 105806, "strand": "+", "frame": 3, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02867", "SO:0001217", "UniRef:UniRef50_B3PM28", "UniRef:UniRef90_D1J7M4" ], "nt": "ATGGCTAAAAAAGTAGTCAAGAAAGCAAAATTACAATTTGCTGCCGGCCAAGCAAAACCAGGCCCAGCATTGGCTGGTGTTGGTATTAACATGCCAGAATTTACAAAGGCATTTAATGACAAAACAAGAGAAAGAGGAAATGAACCAGTTCCTGTTTTAATTACTGTTTATGAAGATAAATCATTTGAATTTAAATTATTTACTTCTCCAACATCATACAAGTTAATCGAAGCTGCCAAAATTAAAAAAGGTAGCGGAGTTGCAAACAAAGAAAAAGTTGGTTCTATAACAATTGATCAATTAAGAGCAATTGCAGAATATAAATTACCTGATTTAAATACAACTAAAATTGATTCAGCAATGAAACAAGTAGCTGGTACTGCAAAGAATATGGGAATTACAGTTGAAGGTTATGACGAATGA", "aa": "MAKKVVKKAKLQFAAGQAKPGPALAGVGINMPEFTKAFNDKTRERGNEPVPVLITVYEDKSFEFKLFTSPTSYKLIEAAKIKKGSGVANKEKVGSITIDQLRAIAEYKLPDLNTTKIDSAMKQVAGTAKNMGITVEGYDE", "aa_hexdigest": "c988e0254890aaae286c794b34012d8d", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7M4", "query_cov": 1.0, "subject_cov": 0.9523809523809523, "identity": 1.0, "score": 271.0, "evalue": 8.2e-92, "valid": true, "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_B3PM28", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM28" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "AHCKFKLKGB_225", "locus": "GCA013367975_00190" }, { "type": "cds", "sequence": "contig_1", "start": 105827, "stop": 106519, "strand": "+", "frame": 2, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "COG:COG0081", "COG:J", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02863", "RefSeq:WP_012855365.1", "SO:0001217", "UniParc:UPI0001BC20A4", "UniRef:UniRef100_D1J7M5", "UniRef:UniRef50_Q4A5E1", "UniRef:UniRef90_D1J7M5" ], "nt": "ATGGCTAAAAGAATTACAAAAAACATTAAAAATGTTAAAAGCTTAGTTAATAAAAAAGACGTATTCCCTCTTGCAGATGCTATTGAATTAGCAAAAAAAGCTTCATTTGCAAAATTTGATGAATCTGTTGATATTGCAATTAGATTAAATCTTGATACAAGAAAATCAGATCAACAATTACGTGGATCAGTTGTACTTCCACACGGTACAGGAAAATCAGTTAAAGTTTTAGTAGCAACTGACGAAGTTTCAGCCCAAAAAGCTTCATTAGAAGCTGGAGCAGATTTTGTTTACAGTTCAGCTGAATTACCAGAAGTATTAAATCAAGATAAATACGATTTTGATGTAATAGTTGCTGATCCTAAAATGATGCTAGTTTTAGGAAAATTTGGTAAAAAATTAGGACCTAAAGGTTTAATGCCAAACCCAAAAACAGGAACAGTTACAATCAATCCTGCTAAAGCTGTTGAAGAACTTAAAAAAGGTAAAGCAAATTATCGTGCTGATAAGGGCGGAATTGTTCATGCCTCATTTGGTAAGAAATCAATGGAAACAAATGTTTTAGCAGAAAATGCCGATACTATTATTCAAACAATTAAGAGATTAAAACCTCAAACAGTTAAGGGTACATATGTTTTAAATATTACCGTTTCAACTTCAATGGGTCAATCTGTTAAAGTAAGAATTGATTAA", "aa": "MAKRITKNIKNVKSLVNKKDVFPLADAIELAKKASFAKFDESVDIAIRLNLDTRKSDQQLRGSVVLPHGTGKSVKVLVATDEVSAQKASLEAGADFVYSSAELPEVLNQDKYDFDVIVADPKMMLVLGKFGKKLGPKGLMPNPKTGTVTINPAKAVEELKKGKANYRADKGGIVHASFGKKSMETNVLAENADTIIQTIKRLKPQTVKGTYVLNITVSTSMGQSVKVRID", "aa_hexdigest": "b6b4b4b8e99890894c640bc5c2c3664f", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC20A4", "ncbi_nrp_id": "WP_012855365.1", "uniref100_id": "UniRef100_D1J7M5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20A4", "RefSeq:WP_012855365.1", "UniRef:UniRef100_D1J7M5" ] }, "ips": { "uniref100_id": "UniRef100_D1J7M5", "uniref90_id": "UniRef90_D1J7M5" }, "psc": { "uniref90_id": "UniRef90_D1J7M5", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_Q4A5E1", "cog_id": "COG0081", "cog_category": "J", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5E1" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [ "rplA" ], "id": "AHCKFKLKGB_226", "locus": "GCA013367975_00191" }, { "type": "cds", "sequence": "contig_1", "start": 106629, "stop": 107021, "strand": "-", "frame": 2, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "nt": "ATGAATAAGAGATTTGGCAACAAAGTTCAATTAGTTGGAGATGACTTAATTGTAACAAATCCAAAATACATTCAAATGGCTATTGACAAACATGCAATCAATGCATCTTTAATAAAGATAAACCAAATAGGTTCATTAACTGAAACATTTAAAGCAATAACAATGTCACAAAAAGCTGGCTTTGTAGCAATAATTTCTCACCGTTCAGGTGAAACTGAAGATACATTTATTGCAGATTTGGCAGTAGCATTAAATACCGGAGAAATCAAAACCGGCTCTCTTTCAAGAACAGATAGAATCGCTAAATACAACCGCTTGTTAAAAATAGAAGCAGAATTAGGCAATGAAGCTAAATTCGAAGGCAAAAACTCATTTTATAATCTAAAAAATTAA", "aa": "MNKRFGNKVQLVGDDLIVTNPKYIQMAIDKHAINASLIKINQIGSLTETFKAITMSQKAGFVAIISHRSGETEDTFIADLAVALNTGEIKTGSLSRTDRIAKYNRLLKIEAELGNEAKFEGKNSFYNLKN", "aa_hexdigest": "a8bfb550153aece1307080461a1bd556", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A139MRN9", "query_cov": 0.9923076923076923, "subject_cov": 0.8376623376623377, "identity": 0.636, "score": 169.0, "evalue": 1.63e-51, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A139PDG5" }, "pscc": { "uniref50_id": "UniRef50_A0A139PDG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "product": "Enolase" }, "genes": [], "id": "AHCKFKLKGB_227", "locus": "GCA013367975_00192" }, { "type": "cds", "sequence": "contig_1", "start": 107037, "stop": 107804, "strand": "-", "frame": 2, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "COG:COG0148", "COG:G", "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_P0DXB0", "UniRef:UniRef90_D1J7M6" ], "nt": "GTGCAATTAGCATGCGATAATGTTAATAATATTATTGCTAAAGCTATTATTGGCAAGGAAGTTTCTAAGCAAGAAGAATTAGATAATTTAATGATTAATCTTGATGGAACTGAAACAAAATCAAAATTAGGGGCAAATGCAATATTAGCTGTTTCATTAGCTATTGCAAAAGCTGGTGCCATTGAAGCAAATCTTCCATTATATAAATATTTGGCAAAATTAAACAACCAAGTAGCATACAAAATGCCAGTGCCAATGCTAAACGTTATTAATGGTGGAGAACATGCTTCAAATACTATTGACTTCCAAGAATTTATGATAATGCCTATTGGGGCAAAAACATTTAAAGAATCATTGCAAATGGCAAACTTCGTATTTCATACATTAGCAAAATTATTGAAACAAGCAGGTCATGGAACACAAGTCGGAGATGAAGGCGGATTTGCACCAAACCTACACACTCATGAAGAAGCATTGGACTTTTTAGTAAATGCGATTAAAGTTGCAGGATATAATCCAGCTACTTCAGGCGAAAAAGCAGTAGCAATTTGTTTGGATGCTGCAAGTTCAGAACTTTATCATTCAGATACAAAATTATATGTGTTTAAAAAATTCAAAAAAGCAATTGAAGAAAAACGTCCAGGATTTGAAAAATATCAAAATGTTAAATATTCATTTACTAGCGATGAATTTGTTGAATATTATGGCCATTTAATTGAGAAATACCCAATAATTTCTATTGAAGATTCACATGATGAAAACGATTGA", "aa": "MQLACDNVNNIIAKAIIGKEVSKQEELDNLMINLDGTETKSKLGANAILAVSLAIAKAGAIEANLPLYKYLAKLNNQVAYKMPVPMLNVINGGEHASNTIDFQEFMIMPIGAKTFKESLQMANFVFHTLAKLLKQAGHGTQVGDEGGFAPNLHTHEEALDFLVNAIKVAGYNPATSGEKAVAICLDAASSELYHSDTKLYVFKKFKKAIEEKRPGFEKYQNVKYSFTSDEFVEYYGHLIEKYPIISIEDSHDEND", "aa_hexdigest": "bfac1696c8cd4a683586cc12527d4998", "start_type": "GTG", "rbs_motif": "GGxGG", "genes": [ "eno" ], "truncated": "both", "pseudogene": { "start": 106734, "stop": 108002, "observations": { "mutation": [ 107039, 107822 ], "stop": [ 107039, 107822 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7M6", "query_cov": 1.0, "subject_cov": 0.5579868708971554, "identity": 0.996, "score": 495.0, "evalue": 7.28e-174, "gene_start": 1, "gene_end": 255, "reference_start": 67, "reference_end": 321, "reference_sequence": "MSKIIKINAYEVLDSRGNPTVKVELNTELAYSEALVPSGASTGSKEALELRDKGTKYENNWFGGKGVQLACDNVNNIIAKAIIGKEVSKQEELDNLMINLDGTETKSKLGANAILAVSLAIAKAGAIEANLPLYKYLAKLNNQVAYKMPVPMLNVINGGEHASNTIDFQEFMIMPIGAKTFKESLQMANFVFHTLAKLLKQAGHGTQVGDEGGFAPNLHTHEEALDFLVNAIKVAGYNPATSGEKAVAICLDAASSELYHSDTKLYVFKKFKKAIEEKRPGFEKYQNVKYSFTSDEFVEYYGHLIEKYPIISIEDSHDENDWDGFIKMNKRFGNKVQLVGDDLIVTNPKYIQMAIDKHAINASLIKINQIGSLTETFKAITMSQKAGFVAIISHRSGETEDTFIADLAVALNTGEIKTGSLSRTDRIAKYNRLLKIESELGNEAKFEGKNSFYNLKN" }, "paralog": false, "identity": 0.995260663507109, "score": 820.0, "evalue": 2.71e-304, "description": "Internal stop codon at 107039, 107822. Nonsense mutation around 107039, 107822.", "psc": { "uniref90_id": "UniRef90_D1J7M6", "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_P0DXB0", "cog_id": "COG0148", "cog_category": "G", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7M6", "EC:4.2.1.11", "UniRef:UniRef50_P0DXB0", "COG:COG0148", "COG:G", "KEGG:K01689", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P0DXB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0DXB0" ], "product": "Enolase" } }, "id": "AHCKFKLKGB_228", "locus": "GCA013367975_00193" }, { "type": "cds", "sequence": "contig_1", "start": 107820, "stop": 108002, "strand": "-", "frame": 2, "gene": null, "product": "Phosphopyruvate hydratase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V5C5X0" ], "nt": "ATGTCAAAAATTATTAAGATTAATGCCTATGAAGTTTTAGATAGTAGAGGAAATCCAACAGTTAAAGTTGAATTAAATACTGAACTTGCATATTCTGAAGCATTAGTACCATCAGGAGCTTCAACAGGATCAAAAGAAGCACTTGAATTACGCGACAAAGGAACAAAATACGAAAATAATTGA", "aa": "MSKIIKINAYEVLDSRGNPTVKVELNTELAYSEALVPSGASTGSKEALELRDKGTKYENN", "aa_hexdigest": "6327a23b922b52ad6b82638088f018a6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A920A3H1", "query_cov": 0.9166666666666666, "subject_cov": 0.873015873015873, "identity": 0.527, "score": 55.1, "evalue": 8.27e-09, "valid": false, "product": "Enolase N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A7V5C5X0" }, "pscc": { "uniref50_id": "UniRef50_A0A7V5C5X0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V5C5X0" ], "product": "Phosphopyruvate hydratase" }, "genes": [], "id": "AHCKFKLKGB_229", "locus": "GCA013367975_00194" }, { "type": "cds", "sequence": "contig_1", "start": 108205, "stop": 108786, "strand": "+", "frame": 1, "gene": null, "product": "DUF3196 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LJ90", "UniRef:UniRef90_D1J7M7" ], "nt": "ATGAATAACATTGATAATAATTTATTTTTTATTGAAGAAGAAATTAAAAAGATTGAAAAAATTGCAAAAGAAGATCCACTTAAGGCAATTTATATTTTAGATGAAGATAGAAAGCAATTCACCCTAAAAGAAGACTTGGATGATTTAGATGCATTGAGGGATAACATTGAATTTCAACTTAAGAAACAAAATTTAATAACCTCAACAAAACTTGATACATTAAGTTTAATAAACAGCTTAAAAAACAAGAAAATGGGTTATGCATTTATGTTGAGCTATAACGAACTTAAAAAGCGAGACGATATAGCAAAATATGCAATTGAATTTCAAGATTTTTTTAAAGGAAATGATTTTGATAGTAAAGGATTTCAAACATTGATTTATGACTTATTAGTCAATAGTAATATTGATTTTGAATACGATATTCAAAACAGAAAAATTAATCCCAAAAAATTAGGATCATTATTAAAGAACGTTGAAATTCAAAAGATGCAAGACGAGATTATTGCAACTTTTGATAAAGATATTTCAAAAAACAAGGTTGCAAGACAAGTGTTTTCAGCATATTTATTTCAAAATTGA", "aa": "MNNIDNNLFFIEEEIKKIEKIAKEDPLKAIYILDEDRKQFTLKEDLDDLDALRDNIEFQLKKQNLITSTKLDTLSLINSLKNKKMGYAFMLSYNELKKRDDIAKYAIEFQDFFKGNDFDSKGFQTLIYDLLVNSNIDFEYDIQNRKINPKKLGSLLKNVEIQKMQDEIIATFDKDISKNKVARQVFSAYLFQN", "aa_hexdigest": "cf1ddd8d92cfb1d1e8e9d680d52b98dd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M7", "query_cov": 1.0, "subject_cov": 0.8143459915611815, "identity": 0.9790000000000001, "score": 364.0, "evalue": 2.11e-126, "valid": true, "uniref50_id": "UniRef50_A0A3G8LJ90" }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LJ90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LJ90" ], "product": "DUF3196 domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_230", "locus": "GCA013367975_00195" }, { "type": "cds", "sequence": "contig_1", "start": 108799, "stop": 108918, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATGAAACAAAAGACGATTATTTAAAAATATCACACGTAATTGAAGTAATTTTAGGTAATCAAACATCAGATTCACTTTCTGAAGAAGAAAAAGAGTTATATAACATTTTTAAATAA", "aa": "MHETKDDYLKISHVIEVILGNQTSDSLSEEEKELYNIFK", "aa_hexdigest": "cf5e792319dd5863fc84e2c6b319ec03", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4597.070999999999, "isoelectric_point": 4.629729270935059 }, "id": "AHCKFKLKGB_231", "locus": "GCA013367975_00196" }, { "type": "cds", "sequence": "contig_1", "start": 109159, "stop": 110391, "strand": "+", "frame": 1, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "COG:COG0544", "COG:O", "EC:5.2.1.8", "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083", "SO:0001217", "UniRef:UniRef50_A0A501XAI0", "UniRef:UniRef90_D1J7M8" ], "nt": "TTGGATAGTATTTTAGATAATATTTATAAAGAACAAATAATTAGCCAAATAACCAAAGATGACGAAATTGTTGGACATGCAAATTTAAGAATTAAAGATATAAATGAAGAAAAAGCAGAAATTTCATTTGTATTTCCACTTTTCCCAGAAGTTAAATTGGGAGATTATAAAAAATTAGGCGTTAAATTAGGATCATGTGAATTAACTGAAAAAGATTTAAATGATGCAAAATCACAAATAGCTTCAAACTATGTAGTTATGCTAGATTCTAAAGAACCAATTAAAGAAAAAGATCAAGTAAACTTTAATTTCAAAGGCTTTATTGACGGAAAACCATTTGATGGCGGAGAAGCAGAAAACTTTGATTTAGTAATTGGATCTAAACAATTTATTCCAGGCTTTGAAGACAAAATGATTGGCCTAAAAGCAGGAGAAGAAAAAGATTTAGATTTAGTATTTCCAGAAGATTATCATGCTAAGAATTTAGCAGGAAAATCTGTTGTTTTCCATGTAAAAATAAATTTTGTTAAAACTGCAAATTATCCAACAATCGATGATAATTTTGTAAAAGAAATTAATTTGCCAAATGTTAAAAATGTTGCTGAATTTGAAGAATATGTAAAATTTATTGCTTTGAAGAACAAATTAGCAAATGTTAAAAATGAATTTATTAACGAAGCAGTTAAAAAATTATCAGAACAATCAGAAGTTTCTTTATCAGAATCATTAGTTGAAGAAGAAGCAGATAAATACTATCAAAATTTCCTTAACAATTTAAAACAACAACAAATTACTGAAAAAGATTATTACGAATTTTCAAAGAGTACAAAAGAAGAATCATTAAATGTATTTAAAAAACAAGCCCAAATAAACTTAAAGCAAATGTTTGTATTAGGTGCTATTGCCAAAGCTGAATCTTTAAAAGTAACATTAGAAGATTATGAAAAAGAAGTTGAAAAATTGGCAAAAACATACGGCTTAGCTCCTGAACATGTAAAACAAATTTTGAAATTTGAAAATGTTGAAAATAATTTAATTAATGAAAGAATTAGCGAAATTTTATTAAAAGAAAATGACACTAAAGCATTTGAAAACTTTGACAAACTATTAAAAGAAGTCGAAGAATATGAAGCTAAACAAACTCAAGCTATTGTAGATGAAGCTAAACGCAAAAGAGAAGAAGCAGAAAAAATAGCTAAAGAATCAAAAGAAAACATTGACGAACAAAAATAA", "aa": "MDSILDNIYKEQIISQITKDDEIVGHANLRIKDINEEKAEISFVFPLFPEVKLGDYKKLGVKLGSCELTEKDLNDAKSQIASNYVVMLDSKEPIKEKDQVNFNFKGFIDGKPFDGGEAENFDLVIGSKQFIPGFEDKMIGLKAGEEKDLDLVFPEDYHAKNLAGKSVVFHVKINFVKTANYPTIDDNFVKEINLPNVKNVAEFEEYVKFIALKNKLANVKNEFINEAVKKLSEQSEVSLSESLVEEEADKYYQNFLNNLKQQQITEKDYYEFSKSTKEESLNVFKKQAQINLKQMFVLGAIAKAESLKVTLEDYEKEVEKLAKTYGLAPEHVKQILKFENVENNLINERISEILLKENDTKAFENFDKLLKEVEEYEAKQTQAIVDEAKRKREEAEKIAKESKENIDEQK", "aa_hexdigest": "a8628f6beb632eb1e50774c69228dd82", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M8", "query_cov": 1.0, "subject_cov": 0.8613445378151261, "identity": 0.993, "score": 759.0, "evalue": 5.8e-275, "valid": true, "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_A0A501XAI0", "cog_id": "COG0544", "cog_category": "O", "go_ids": [ "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501XAI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501XAI0" ], "product": "Trigger factor" }, "genes": [ "tig" ], "id": "AHCKFKLKGB_232", "locus": "GCA013367975_00197" }, { "type": "cds", "sequence": "contig_1", "start": 110483, "stop": 111709, "strand": "+", "frame": 2, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038", "KEGG:K03070", "SO:0001217", "UniRef:UniRef50_Q98RA6", "UniRef:UniRef90_D1J7M9" ], "nt": "ATGAAAATATTTGAATTTAAATCAACAGAAATGAGAATTGCAGAGGCAACCCTTAAGAGGATAAATGCTTTAGAACCAGAAATATCAAAATTAAGCAACGAGGAATTGCAACAAAAAACTATTGAGTTTAAGTCGCGTTTAAAGGCAGGAGAAACGCCAGAACAAATTAGACCTGAAACGTTTGCTGTTTGTAGAGAAGCAACAAAACGTATTTTAGGCAAAAGACCATTTGATGTTCAAATGATAGGCGGAATAATTTTAGATTTAGGTTCTGTAGCAGAAATGAAAACCGGTGAAGGTAAAACTATTACTTCTATAGCACCAATTTATTTAAACGCAATTACTGGTCAATCTGTTATTGTTTCAACAGTTAATGAATATCTAGCAGAACGTGATGCTCAAGAAATGGGGCAAGTTTTTAACTTTTTAGGTTTAAGTGTTGGGATAAACAAATCTCAAATGTCAAGCGAGTTAAAAAGAGAAGCATATGCTTGCGATATTGTTTATTCTGTTCATTCTGAATTGGGTTTTGACTATTTACGTGACAATATGGCAATGAGTAAAGAAGAAAAAGTTCAACGTGGGCTTGATTTTATTTTACTAGATGAAGTAGATTCAATATTGATTGATGAAGCAAAAACTCCTTTAATTATTTCAGGTGGGGATAATGACGAAAGCAATTTGTATACAATTGCAGATTTGTTTGTAAGAACATTAAGTCAAGATGATTATTTTATTGATGAAGAAACAAAATCTGTTTACCTAACAGATAAAGGAATTGAAAAAGCTAATAAATATTTCAATTTTAAAAATCTTTATGATATAGAAAATTCTGAACTTGTTCATAGAATTCAAAATGCTTTAAGAGCACATAAAGTAATGAAATTAGATGTTGAATATATTGTTAGAAACGACAAAATTGAATTAGTTGATTCCTTCACAGGTAGAATAATGGAAGGAAGAGCTTATTCAGAAGGATTACAACAAGCAATTCAAGCAAAAGAACGTGTTGAAATTGAATCAGAAACCAAAACATTAGCAACAATCACTTATCAAAATTTTTTCCGTTTATTTAAAAAAATAAGCGGTATGACAGGAACAGCAAAAACAGAAGAAAAAGAATTTATTGATATTTATAATATGAGGGTCAATGTTGTTCCAACAAATAAACCAATAGCTAGATTTGATGATAAAGATGAAATCTATGTTGATATGCATAGCAAATGA", "aa": "MKIFEFKSTEMRIAEATLKRINALEPEISKLSNEELQQKTIEFKSRLKAGETPEQIRPETFAVCREATKRILGKRPFDVQMIGGIILDLGSVAEMKTGEGKTITSIAPIYLNAITGQSVIVSTVNEYLAERDAQEMGQVFNFLGLSVGINKSQMSSELKREAYACDIVYSVHSELGFDYLRDNMAMSKEEKVQRGLDFILLDEVDSILIDEAKTPLIISGGDNDESNLYTIADLFVRTLSQDDYFIDEETKSVYLTDKGIEKANKYFNFKNLYDIENSELVHRIQNALRAHKVMKLDVEYIVRNDKIELVDSFTGRIMEGRAYSEGLQQAIQAKERVEIESETKTLATITYQNFFRLFKKISGMTGTAKTEEKEFIDIYNMRVNVVPTNKPIARFDDKDEIYVDMHSK", "aa_hexdigest": "ae83a3a804cfa4d25aff8f17034ae6fd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034361D20", "query_cov": 0.9534313725490197, "subject_cov": 0.9328537170263789, "identity": 0.584, "score": 445.0, "evalue": 3.84e-152, "valid": false, "product": "DEAD/DEAH box helicase", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_A0A939UGC5", "kegg_orthology_id": "K03070" }, "genes": [ "secA" ], "truncated": "3-prime", "pseudogene": { "start": 110483, "stop": 112012, "observations": { "mutation": [ 111707 ], "stop": [ 111707 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7M9", "query_cov": 1.0, "subject_cov": 0.4722222222222222, "identity": 1.0, "score": 780.0, "evalue": 2.28e-277, "gene_start": 1, "gene_end": 408, "reference_start": 1, "reference_end": 408, "reference_sequence": "MKIFEFKSTEMRIAEATLKRINALEPEISKLSNEELQQKTIEFKSRLKAGETPEQIRPETFAVCREATKRILGKRPFDVQMIGGIILDLGSVAEMKTGEGKTITSIAPIYLNAITGQSVIVSTVNEYLAERDAQEMGQVFNFLGLSVGINKSQMSSELKREAYACDIVYSVHSELGFDYLRDNMAMSKEEKVQRGLDFILLDEVDSILIDEAKTPLIISGGDNDESNLYTIADLFVRTLSQDDYFIDEETKSVYLTDKGIEKANKYFNFKNLYDIENSELVHRIQNALRAHKVMKLDVEYIVRNDKIELVDSFTGRIMEGRAYSEGLQQAIQAKERVEIESETKTLATITYQNFFRLFKKISGMTGTAKTEEKEFIDIYNMRVNVVPTNKPIARFDDKDEIYVDMHSKWKAVTEEVKRVYKKKQPILIGTAQVEDSEILHEYLLSEGIPHTVLNAKQDASEAEIISKAGQVGAVTIATNMAGRGTDIKPSKEALELGGLYVLGTEKAESRRIDNQLKGRSGRQGDVGYTKFFLSLDDQLILRFSVQDHWKEIFKEYGEDPIPGEAIRKAFLRAQKKIEGFNFDNRKSVLNFDDVIRQQRDLIYEQRDLILNRDDLGTIIRKMISVAAEKTVNNPFFIKNNSLDLIEFVKHLNNNFMILTNKEFSVEELVEYDREELIQYLIETWNKCYDQLRQNIIDKYGISSLINSERNIILSVFDAAWQDHINIMDRLRRSTNLVQYSQKNPYQVYTQLGSKKFKELTDRIALECTINLMNNYDAIPSTNTDVDLSWLTNAFEEKTDMAQTTSKNQQALEFINYLLKSERDHLIKEGLGEQEVDDKLKEVERSILKEFQFQLASLQTNEEKK" }, "paralog": false, "identity": 0.9980353634577603, "score": 979.0, "evalue": 0.0, "description": "Internal stop codon at 111707. Nonsense mutation around 111707.", "psc": { "uniref90_id": "UniRef90_D1J7M9", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_Q98RA6", "cog_id": "COG0653", "cog_category": "U", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7M9", "EC:7.4.2.8", "UniRef:UniRef50_Q98RA6", "COG:COG0653", "COG:U", "KEGG:K03070", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RA6" ], "product": "Protein translocase subunit SecA" } }, "id": "AHCKFKLKGB_233", "locus": "GCA013367975_00198" }, { "type": "cds", "sequence": "contig_1", "start": 111809, "stop": 112129, "strand": "+", "frame": 2, "gene": "secA", "product": "SecA family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920T749" ], "nt": "TTGCTATCTGAAGGCATACCTCATACAGTTTTAAATGCAAAGCAAGATGCTTCGGAAGCAGAAATTATTTCTAAAGCAGGACAAGTAGGAGCAGTTACTATTGCCACAAACATGGCTGGACGTGGAACAGATATCAAGCCTTCAAAAGAAGCACTAGAATTAGGTGGATTGTATGTTTTAGGAACTGAAAAAGCTGAATCGAGAAGAATTGATAATCAACTAAAAGGACGTTCTGGTCGTCAAGGAGATGTAGGATATACAAAATTCTTTTTATCATTAGATGACCAATTGATTTTGCGTTTTTCAGTTCAAGATCACTGA", "aa": "MLSEGIPHTVLNAKQDASEAEIISKAGQVGAVTIATNMAGRGTDIKPSKEALELGGLYVLGTEKAESRRIDNQLKGRSGRQGDVGYTKFFLSLDDQLILRFSVQDH", "aa_hexdigest": "4cd803eb0ed4b784c964843ec9d98f33", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920T749", "query_cov": 0.8113207547169812, "subject_cov": 0.9247311827956989, "identity": 0.698, "score": 119.0, "evalue": 2.96e-33, "valid": false, "product": "SecA family profile domain-containing protein", "uniref50_id": "UniRef50_A0A920T749" }, "pscc": { "uniref50_id": "UniRef50_A0A920T749", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920T749" ], "product": "SecA family profile domain-containing protein" }, "genes": [ "secA" ], "id": "AHCKFKLKGB_234", "locus": "GCA013367975_00199" }, { "type": "cds", "sequence": "contig_1", "start": 112193, "stop": 112534, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGGCTCAAAAAAAGATTGAAGGGTTTAACTTTGATAATAGAAAAAGCGTCCTAAATTTCGATGACGTAATTAGACAACAACGTGATCTAATTTATGAACAACGTGATCTAATTTTAAATAGAGATGATCTTGGAACAATAATTAGAAAAATGATATCAGTTGCAGCAGAAAAAACAGTAAATAACCCATTCTTTATAAAAAACAATAGTTTGGATTTAATAGAATTTGTAAAGCATCTAAATAACAATTTTATGATTTTAACAAACAAGGAATTTAGTGTTGAAGAATTAGTTGAATATGATCGTGAAGAATTAATTCAATACTTAATTGAAACTTGA", "aa": "MRAQKKIEGFNFDNRKSVLNFDDVIRQQRDLIYEQRDLILNRDDLGTIIRKMISVAAEKTVNNPFFIKNNSLDLIEFVKHLNNNFMILTNKEFSVEELVEYDREELIQYLIET", "aa_hexdigest": "c4260b48df972497f5fd3f9d24e28613", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13543.310099999984, "isoelectric_point": 5.040334129333496 }, "id": "AHCKFKLKGB_235", "locus": "GCA013367975_00200" }, { "type": "cds", "sequence": "contig_1", "start": 112661, "stop": 113077, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAGATTAAGAAGAAGTACAAATTTAGTTCAATATTCTCAAAAGAACCCGTATCAAGTTTACACTCAATTGGGTTCAAAAAAATTCAAAGAATTAACAGACAGAATTGCTTTGGAATGTACAATTAATTTAATGAATAATTACGACGCAATACCTTCAACAAATACTGATGTTGATTTATCATGGTTAACAAATGCTTTTGAAGAAAAAACAGATATGGCTCAAACAACTTCAAAGAACCAACAAGCTCTTGAATTTATCAATTATTTATTGAAGTCAGAACGTGATCATTTGATTAAAGAAGGTTTAGGCGAACAAGAAGTGGATGATAAACTAAAGGAAGTGGAAAGATCAATATTAAAAGAGTTCCAATTCCAACTTGCTTCTTTGCAAACTAATGAAGAAAAAAAATAA", "aa": "MDRLRRSTNLVQYSQKNPYQVYTQLGSKKFKELTDRIALECTINLMNNYDAIPSTNTDVDLSWLTNAFEEKTDMAQTTSKNQQALEFINYLLKSERDHLIKEGLGEQEVDDKLKEVERSILKEFQFQLASLQTNEEKK", "aa_hexdigest": "fdd96d929476e5d5db4aad5b81cc4d01", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16172.984399999992, "isoelectric_point": 5.129798698425293 }, "id": "AHCKFKLKGB_236", "locus": "GCA013367975_00201" }, { "type": "cds", "sequence": "contig_1", "start": 113085, "stop": 113333, "strand": "-", "frame": 2, "gene": "yjhB", "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)", "db_xrefs": [ "COG:COG1051", "COG:F", "EC:3.6.1.17", "KEGG:K01518", "SO:0001217", "UniRef:UniRef50_D1J7N0", "UniRef:UniRef90_D1J7N0" ], "nt": "TTGCTCAAGGGTTTTAGAGAAGTCAATAAATATATTATTGGAAAATTAATATCTAAAGAAGTTGTTTATTTTATTGCTAAGCCAACATCTTTTGATTTAATTAAGCAAGATAGTGAAATTAAGGTAGTTGAATGGCAAGATACAACAAATGCATTGTTATATCGCATAACATACGAAAGTGATTGTGAAATATTGAAAAAGGCTGTTGAATTTTATTGCGAATATATAACAAAAAATAACGAGTTTTAA", "aa": "MLKGFREVNKYIIGKLISKEVVYFIAKPTSFDLIKQDSEIKVVEWQDTTNALLYRITYESDCEILKKAVEFYCEYITKNNEF", "aa_hexdigest": "e842433637f69956c87135a143581f8c", "start_type": "TTG", "rbs_motif": null, "genes": [ "yjhB" ], "truncated": "3-prime", "pseudogene": { "start": 113085, "stop": 113516, "observations": { "mutation": [ 113426 ], "stop": [ 113426 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N0", "query_cov": 1.0, "subject_cov": 0.5734265734265734, "identity": 0.988, "score": 167.0, "evalue": 4.67e-52, "gene_start": 1, "gene_end": 82, "reference_start": 62, "reference_end": 143, "reference_sequence": "MKKEKSCGAIIFKEENDNLFVLLVEQTAGHWGFPKGHVEKNETEEETAIREVKEETNIDIKLLKGFREVNKYIIGKLISKEVVYFIAKPTSFDLIKQDSEIKVVEWQDTTNALLYRITYESDCEILKKAVEFYCEYITKNNEF" }, "paralog": false, "identity": 0.993006993006993, "score": 285.0, "evalue": 7.48e-101, "description": "Internal stop codon at 113426. Nonsense mutation around 113426.", "psc": { "uniref90_id": "UniRef90_D1J7N0", "gene": "YjhB", "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)", "ec_ids": [ "3.6.1.17" ], "uniref50_id": "UniRef50_D1J7N0", "cog_id": "COG1051", "cog_category": "F", "kegg_orthology_id": "K01518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N0", "EC:3.6.1.17", "UniRef:UniRef50_D1J7N0", "COG:COG1051", "COG:F", "KEGG:K01518" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N0" ], "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)" } }, "id": "AHCKFKLKGB_237", "locus": "GCA013367975_00202" }, { "type": "cds", "sequence": "contig_1", "start": 113424, "stop": 113516, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGAGAAGTCGTGTGGCGCTATTATTTTCAAAGAAGAAAATGATAATCTTTTTGTTTTATTAGTTGAACAAACAGCAGGCCATTGA", "aa": "MKKEKSCGAIIFKEENDNLFVLLVEQTAGH", "aa_hexdigest": "ac70932b5b7a134afe7ea7e4125d559a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3392.8976999999995, "isoelectric_point": 5.573540687561034 }, "id": "AHCKFKLKGB_238", "locus": "GCA013367975_00203" }, { "type": "cds", "sequence": "contig_1", "start": 113714, "stop": 114106, "strand": "-", "frame": 3, "gene": null, "product": "DUF4199 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U5", "UniRef:UniRef90_A0A454C9U5" ], "nt": "ATGGATATTAACAATATTATAAAAAATAATATAAAAAGATTATTAATAACAACCGCATTTGGCTTTTTAGCGATTTTGATTTCTTTTTTGATTACCGGGATATTTTCAATACAATCACTTTCAAATAATAGCCGTCTTGCAATATCTTGCTTTGGACAAATAATTTGCGGTTTTTATGTTGTTTATTATATTTTAAATTTCTTTTTAGCATATAGATTTAAATCAGAAATTGCAAACAAAAAGATATTAAAGACTCTATTAATAATCAGCATTTTTATTTTGATATGTGGAATTTGTTTTATTGTTAATGGTTTGATTTTACCTTCAATTAGAAAAACATACATTCAAGCAGTAATGACTAAAAATAATAATTTATTACAAAGTATACTTTGA", "aa": "MDINNIIKNNIKRLLITTAFGFLAILISFLITGIFSIQSLSNNSRLAISCFGQIICGFYVVYYILNFFLAYRFKSEIANKKILKTLLIISIFILICGICFIVNGLILPSIRKTYIQAVMTKNNNLLQSIL", "aa_hexdigest": "f20def24de83c98f5d2a756e7d4ab2ab", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 113603, "stop": 114106, "observations": { "mutation": [ 113686, 113716 ], "stop": [ 113686, 113716 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9U5", "query_cov": 1.0, "subject_cov": 0.7784431137724551, "identity": 0.985, "score": 237.0, "evalue": 2.78e-78, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MDINNIIKNNIKRLLITTAFGFLAILISFLITGIFSIQSLSSNSRLAISCFGQIICGFYVVYYILNFFLVYRFKSEIANKKILKTLLIISIFILICGICFIVNGLILPSIRKTYIQAVMTKNNNLLQSILWQGKVIKVEMWISSTIFLVAIASSLILCSFIFKKMNN" }, "paralog": false, "identity": 0.9700598802395209, "score": 307.0, "evalue": 1.49e-108, "description": "Internal stop codon at 113686, 113716. Nonsense mutation around 113686, 113716.", "psc": { "uniref90_id": "UniRef90_A0A454C9U5", "product": "DUF4199 domain-containing protein", "uniref50_id": "UniRef50_A0A454C9U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9U5", "UniRef:UniRef50_A0A454C9U5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U5" ], "product": "DUF4199 domain-containing protein" } }, "id": "AHCKFKLKGB_239", "locus": "GCA013367975_00204" }, { "type": "cds", "sequence": "contig_1", "start": 114190, "stop": 115074, "strand": "+", "frame": 1, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "COG:COG2812", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360", "KEGG:K02343", "SO:0001217", "UniRef:UniRef50_D1J7N2", "UniRef:UniRef90_D1J7N2" ], "nt": "GTGGCACTTACTAATAAATATTTAGCATTGTATAGAAAATATAGACCAAAAATTTTTGATGATATAAAAGGCCAAGATCACATTGTAACAACATTGAAAAATATTATTTTGAATAATAAATTGAGTCATGCGTATTTATTTTGTGGTCCCCATGGAAATGGAAAAACTTCAACTGCTAAAGTTTTTGCAAACACAATTAATTGTTTACATAATCAAAGCGATATATTAAAACCATGTGAAGAATGTATCAAAAATGTCGATAGAAATCTAGATATTATAGAAATAGATGCTGCTTCTAATACCGGCATTGATGACATTAGAGAATTAAAAGAAAAAATAAAACATTTACCAACACAAAGCAAATATAAAATTTATATTATTGATGAAGTTCATATGCTTTCAAAAGCAGCCTTTAACGCTTTATTAAAAACAATTGAAGATCCACCTAGTCACATAATTTTTATTCTAGCAACAACTGATCCCCAAAAAATACCAGAAACTATTTTAAGTAGGGTTCAAAGATTCAATTTTAAACTTATGACAATTGAAACATTAGTTTCTCAATTGAAGGATATTTTCAATAAAGAAAATATTGAATATGAAGATGATGCAATTAATGTTATAGCTAAATTAGGAAATGGTAGCCTTAGAGATTCATTGAGTATTGCAGACCATGTTGCAATATATTGTGCCAATAAAGTTATTAAGAAAGAAGTTGTTGAACAATTATTTGGAATTGTAAATATAGATAATAAAATAGAGTTATTAAATTGTATGTCAATGCATGATATCAATAAAACATTGAATCTTGCAAGAGAATTATTTAGTCAAGGTATTGATATAAACATCTTGATAAACGATTTAATAAACATTTTAAAAGAATGA", "aa": "MALTNKYLALYRKYRPKIFDDIKGQDHIVTTLKNIILNNKLSHAYLFCGPHGNGKTSTAKVFANTINCLHNQSDILKPCEECIKNVDRNLDIIEIDAASNTGIDDIRELKEKIKHLPTQSKYKIYIIDEVHMLSKAAFNALLKTIEDPPSHIIFILATTDPQKIPETILSRVQRFNFKLMTIETLVSQLKDIFNKENIEYEDDAINVIAKLGNGSLRDSLSIADHVAIYCANKVIKKEVVEQLFGIVNIDNKIELLNCMSMHDINKTLNLARELFSQGIDINILINDLINILKE", "aa_hexdigest": "599f28bc4d3f563ffeffba8cd9517212", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00244E6DAE", "query_cov": 0.8163265306122449, "subject_cov": 0.967741935483871, "identity": 0.629, "score": 304.0, "evalue": 8.38e-101, "valid": false, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A7V0IXX6", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "genes": [ "dnaX" ], "truncated": "3-prime", "pseudogene": { "start": 114190, "stop": 115377, "observations": { "mutation": [ 115072 ], "stop": [ 115072 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N2", "query_cov": 1.0, "subject_cov": 0.4407796101949025, "identity": 1.0, "score": 579.0, "evalue": 4.82e-203, "gene_start": 1, "gene_end": 294, "reference_start": 1, "reference_end": 294, "reference_sequence": "MALTNKYLALYRKYRPKIFDDIKGQDHIVTTLKNIILNNKLSHAYLFCGPHGNGKTSTAKVFANTINCLHNQSDILKPCEECIKNVDRNLDIIEIDAASNTGIDDIRELKEKIKHLPTQSKYKIYIIDEVHMLSKAAFNALLKTIEDPPSHIIFILATTDPQKIPETILSRVQRFNFKLMTIETLVSQLKDIFNKENIEYEDDAINVIAKLGNGSLRDSLSIADHVAIYCANKVIKKEVVEQLFGIVNIDNKIELLNCMSMHDINKTLNLARELFSQGIDINILINDLINILKEWIIFNKTRDQSLVSTDFKILLQIKIDDVKAHYYIETLIDQLHSIAISDSPEDYFEILLLKLCNYLSPKEEYKNSAVMPKISNEEFNNFNSQEKQVINKNNIYKSLDLSSIANGFNANNSTIQNSSDNNSTEDLANNSKKIDDLLEKTAEIYSLDQTSEFEVADISNQIISNNQISIDSNVDNQETADLQISENWKDLVNEDLISKENSENKHVPLETIIDCLLIKQYLKNNMLNDGRSNFTDIDAIKYGMLSQKITDKNEINILSDFKIIFSTNDFILLTCDIDEKVYDLNINSHNENIMAALNKVFDRYLYAYAITKGEILEAKKYWKANINEIRAKKVKPLEDLNLKYNKTVQKDVAWAQEIFGDKFKLNK" }, "paralog": false, "identity": 0.9873417721518988, "score": 773.0, "evalue": 1.05e-281, "description": "Internal stop codon at 115072. Nonsense mutation around 115072.", "psc": { "uniref90_id": "UniRef90_D1J7N2", "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_D1J7N2", "cog_id": "COG2812", "cog_category": "L", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N2", "EC:2.7.7.7", "UniRef:UniRef50_D1J7N2", "COG:COG2812", "COG:L", "KEGG:K02343", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N2" ], "product": "DNA polymerase III subunit gamma/tau" } }, "id": "AHCKFKLKGB_240", "locus": "GCA013367975_00205" }, { "type": "cds", "sequence": "contig_1", "start": 115132, "stop": 115653, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAATTAAAATTGATGATGCTAAAGCACACTATTACATTGAAACATTGATAGATCAATTGCATAGTATTGCCGTAAGTGATAGTCCAGAAGATTATTTTGAAATTCTGTTGCTAAAGTTATGTAATTACTTAAGTCCAAAAGAAGAATACAAAAATTCTGCAGTAATGCCAAAAATTAGCAATGAAGAATTTAATAATTTTAATAGTCAAGAAAAACAGACCATTAATAAAAATAATATTTACAAATCATTGGATTTATCCTCAATAGCAAATGGATTCAATGCAAACAATTCCGCAATACAAAATTCAAGTGATAATAATTCAACAGAAGACTTAGCTAATAATTCAAAAAAGATTGATGACTTATTAGAAAAAACTGCAGAAATATATTCATTAGATCAGACATCAGAGTTTGAAGTTGCTGATATTTCTAATCAAATAATAAGTAATAATCAAATTAGCATAGATTCAAATGTTGATAACCAAGAAACATCCGATTTACAAATATCGGAAAATTGA", "aa": "MQIKIDDAKAHYYIETLIDQLHSIAVSDSPEDYFEILLLKLCNYLSPKEEYKNSAVMPKISNEEFNNFNSQEKQTINKNNIYKSLDLSSIANGFNANNSAIQNSSDNNSTEDLANNSKKIDDLLEKTAEIYSLDQTSEFEVADISNQIISNNQISIDSNVDNQETSDLQISEN", "aa_hexdigest": "c737dcf77decdea4ce7f7e897e2c27d7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19556.055199999988, "isoelectric_point": 4.198037147521972 }, "id": "AHCKFKLKGB_241", "locus": "GCA013367975_00206" }, { "type": "cds", "sequence": "contig_1", "start": 115714, "stop": 116055, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAACAATAATCGACTGTTTATTAATAAAACAATATTTGAAAAACAATATGTTAAATGATGGTCGTAGTAATTTTACAGACATTGATGCTATTAAATATGGTATGTTAAGTCAAAAAATAACAGACAAAAATGAAATTAATATCTTATCAGACTTTAAAATTATTTTTTCAACGAATGATTTTATTTTGTTAACGTGTGATATAGATGAAAAAGTCTACGATTTAAATATAAATTCACATAATGAAAATATTATGGCTGCATTGAATAAAGTTTTTGATAGATATCTTTATGTTTATGCCATTACAAAAGATGAGATATTAGAAGCAAAGAAATATTGA", "aa": "METIIDCLLIKQYLKNNMLNDGRSNFTDIDAIKYGMLSQKITDKNEINILSDFKIIFSTNDFILLTCDIDEKVYDLNINSHNENIMAALNKVFDRYLYVYAITKDEILEAKKY", "aa_hexdigest": "44fdef39c406177904bc089d2d8ce400", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13245.102699999985, "isoelectric_point": 4.749602699279785 }, "id": "AHCKFKLKGB_242", "locus": "GCA013367975_00207" }, { "type": "cds", "sequence": "contig_1", "start": 116217, "stop": 116516, "strand": "+", "frame": 3, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "KEGG:K09747", "RefSeq:WP_012855373.1", "SO:0001217", "UniParc:UPI0001BC20AC", "UniRef:UniRef100_D1J7N3", "UniRef:UniRef50_Q98RF9", "UniRef:UniRef90_D1J7N3" ], "nt": "ATGAGTATGAACATGAATGAAATGCTTAAAAAAGCACGTAGAATTCAAACTGAAATGGAAAAAGATGAACAAGAAATTCAAAAGAAAGAATTTACTATTGAAAAGCAAGGTATAACCGTTGTTATGAATGGTTTGAGAAAATTAGTGTCTATAAAATTTAATCCTGCATTAATCGATCCAGATGATCCTGAATTATTAGAAGATTTATTAATGCTTGCGGTTAATGAAGCAATAGAAACAGTTGATAAGGCTTTTGATGAAATTAATGCTAAATATTCTGATAGTGGCCTTTCATTCTAA", "aa": "MSMNMNEMLKKARRIQTEMEKDEQEIQKKEFTIEKQGITVVMNGLRKLVSIKFNPALIDPDDPELLEDLLMLAVNEAIETVDKAFDEINAKYSDSGLSF", "aa_hexdigest": "77242d963041dcb031811ba880313b74", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20AC", "ncbi_nrp_id": "WP_012855373.1", "uniref100_id": "UniRef100_D1J7N3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20AC", "RefSeq:WP_012855373.1", "UniRef:UniRef100_D1J7N3" ] }, "ips": { "uniref100_id": "UniRef100_D1J7N3", "uniref90_id": "UniRef90_D1J7N3" }, "psc": { "uniref90_id": "UniRef90_D1J7N3", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_Q98RF9", "cog_id": "COG0718", "cog_category": "K", "kegg_orthology_id": "K09747", "go_ids": [ "GO:0003677" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RF9" ], "product": "Nucleoid-associated protein MYPU_0500" }, "genes": [ "ybaB" ], "id": "AHCKFKLKGB_243", "locus": "GCA013367975_00208" }, { "type": "cds", "sequence": "contig_1", "start": 116516, "stop": 117106, "strand": "+", "frame": 2, "gene": "recR", "product": "Recombination protein RecR", "db_xrefs": [ "COG:COG0353", "COG:L", "RefSeq:WP_175933397.1", "SO:0001217", "UniParc:UPI00158CF438", "UniRef:UniRef100_UPI00158CF438", "UniRef:UniRef50_D1J7N4", "UniRef:UniRef90_D1J7N4" ], "nt": "ATGAACAATATTGAGATACAAAAACTAGAAGAACTTTTAAAAACGGTTTTTGATTTTACTAAAAAACAATCTCAAAAATTTATATTCAACATTTTTAAATCATCAAGTGAAGATATAGATCAAATTTGCAATCAAATAAAAGTATTGAGAAATTCAATAAAGAAGTGTAATATTTGCAATAGATATAACGAAAATACAATTTGCAATATTTGCTTGGACACTAATCGAACAAAACAACTTATGTTAGTTGGCAATTCGTTAGATATTGATAAATTTGAAAATATTGGTTTGTATAAAGGAAAATATTTTGTATTTGATCAAAATATAAGCTTAAAGAATGAAGATTTTATAGTTTCAAGTTTATTAGAAAAATTAATAGATATATTAAAAGATAAGAATGAACTAATTCTTTCACTTTCCCCAACTATTGAGGGTCAATTTAATATGCATTATATTAAAAAATTCTTGAAAAACAAAATAAATTTTACAAATGTTTTTCAATTGTCAACTGGTATTCCTTTGGGCTCATCAGTTGAATATATTGATCAAGATACATTAAAAGAATCATTATTGAATAAAATCAAAATGTAG", "aa": "MNNIEIQKLEELLKTVFDFTKKQSQKFIFNIFKSSSEDIDQICNQIKVLRNSIKKCNICNRYNENTICNICLDTNRTKQLMLVGNSLDIDKFENIGLYKGKYFVFDQNISLKNEDFIVSSLLEKLIDILKDKNELILSLSPTIEGQFNMHYIKKFLKNKINFTNVFQLSTGIPLGSSVEYIDQDTLKESLLNKIKM", "aa_hexdigest": "27719367b43cfa05d788c29f3354d35e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158CF438", "ncbi_nrp_id": "WP_175933397.1", "uniref100_id": "UniRef100_UPI00158CF438", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158CF438", "RefSeq:WP_175933397.1", "UniRef:UniRef100_UPI00158CF438" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158CF438", "uniref90_id": "UniRef90_D1J7N4" }, "psc": { "uniref90_id": "UniRef90_D1J7N4", "gene": "RecR", "product": "Recombination protein RecR", "uniref50_id": "UniRef50_D1J7N4", "cog_id": "COG0353", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_D1J7N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N4" ], "product": "Recombination protein RecR" }, "genes": [ "recR" ], "id": "AHCKFKLKGB_244", "locus": "GCA013367975_00209" }, { "type": "cds", "sequence": "contig_1", "start": 117121, "stop": 117381, "strand": "+", "frame": 1, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0006233", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_A0A448ZVP3", "UniRef:UniRef90_D1J7N5" ], "nt": "ATGTCAAAGGGAAAATTTGTAGTATTTGAAGGGATGGATGGTTCAGGAAAAACAACTATTATAAAGAAATTAAAAGAATATTTAGAGTCTAAAAATAGACTAGATGATTTCGTATTTACAAGAGAACCAGGAAGCTCACATTCAAAAGAAGCAGAAGAAATAAGACAGCTAATTTTAGATAATGATAATTCTTTTAGTGTTATGGTGGATGCTTTGTTATTTGCAGCAAGTAGAAGGTTAAATCTTGAAAAATGCGTTTGA", "aa": "MSKGKFVVFEGMDGSGKTTIIKKLKEYLESKNRLDDFVFTREPGSSHSKEAEEIRQLILDNDNSFSVMVDALLFAASRRLNLEKCV", "aa_hexdigest": "5e432a091c89f9b807b2724ce10618be", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [ "tmk" ], "truncated": "3-prime", "pseudogene": { "start": 117121, "stop": 117684, "observations": { "mutation": [ 117379, 117424, 117649 ], "stop": [ 117379, 117424, 117649 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N5", "query_cov": 1.0, "subject_cov": 0.40375586854460094, "identity": 0.988, "score": 167.0, "evalue": 6.82e-51, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MSKGKFVVFEGMDGSGKTTIIKKLKEYLESKNRLDDFIFTREPGSSHSKEAEEIRQLILDNDNSFSVMVDALLFAASRRLNLEKCVWPGLEKNKTVISDRYWTSSFVYQGVLGGLGLKKVKDINNIATEETTPDLTIFFDMEPELSVERITQIRKTKTDRLESTNVEYYKKLRDSYWYVINQGNEKYEIIKSDCNIDELFKRVLNVLKENKII" }, "paralog": false, "identity": 0.9786096256684492, "score": 358.0, "evalue": 1.24e-128, "description": "Internal stop codon at 117379, 117424, 117649. Nonsense mutation around 117379, 117424, 117649.", "psc": { "uniref90_id": "UniRef90_D1J7N5", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_A0A448ZVP3", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0006233" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N5", "EC:2.7.4.9", "UniRef:UniRef50_A0A448ZVP3", "COG:COG0125", "COG:F", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0006233" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVP3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVP3" ], "product": "Thymidylate kinase" } }, "id": "AHCKFKLKGB_245", "locus": "GCA013367975_00210" }, { "type": "cds", "sequence": "contig_1", "start": 117541, "stop": 117651, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCCAGAACTATCAGTTGAAAGAATTACGCAAATTCGAAAAACAAAAACTGATAGATTAGAATCTACCAATGTTGAATATTACAAAAAACTAAGAGATTCTTATTGA", "aa": "MEPELSVERITQIRKTKTDRLESTNVEYYKKLRDSY", "aa_hexdigest": "a43bed897f7f5d7fb1812dc0843d9f5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4420.9522000000015, "isoelectric_point": 8.064688682556149 }, "id": "AHCKFKLKGB_246", "locus": "GCA013367975_00211" }, { "type": "cds", "sequence": "contig_1", "start": 117800, "stop": 118633, "strand": "+", "frame": 2, "gene": "dnaX", "product": "DNA polymerase III subunit", "db_xrefs": [ "COG:COG2812", "COG:L", "RefSeq:WP_175933398.1", "SO:0001217", "UniParc:UPI00158B3F63", "UniRef:UniRef100_UPI00158B3F63", "UniRef:UniRef50_D1J7N6", "UniRef:UniRef90_D1J7N6" ], "nt": "ATGATAAACAAAAATTTGAGTAAAGTATTGGAAAATTCAATAAAAAACAATAAGCTCCAACAAGTTTATTTACTATTTTCACGTGCAAATCAAGATATTGATTTAGATATTTTAGAATTTGTTAGATTAGTTAATGATGAAAAACAAATAAGCTGGCATCAATGTTTGCAAAAAGAATATTGTTTTATTATTGATGGGGGCCAAGGCGAAATATCGAAAGAAAATTTACAGGAATTTTTAAATCGTGTTTCCAAAAGCTCTCTATTTGCTAATAAGTATAAATTTTTAATTATTAAGTGTGTTGAAAATATTACACTAAAGGCCGCAAATAGTATTTTGAAATTTTTAGAAAATCCTCCATTTAATACAATAATATTATTACATACAATAAAGCCTCAAAACGTTTTGAAAACTATCAAATCAAGATGTTTTATTATTGATATTCGTGATGATTTTAATGCTGTAGCTAGCGTTGAAAGTTTTTTTATTACATATAAAATTGACGTAAGCAAGCAAAAACAATTTAGGGAACTTGCTGATTTAATATACAATTCACCAGCAAATCTATTTAATCTTATTTTGTTTTATATTCAACAATTAAATATAGAAAATTATAAAAATATTATTCATTTTTCTATTTTTATTTTTAAACAAATTTATGAATTAAAAAATAGTATAAAAAAGGATAAAAATATTAGTAAATTTGATTCTATTTCAATATTAAATATTATTAATAATTTATATAATTTTGTTGAATTAATTGATAACAATAATTGTATTTTCAATATTCAAAAAGCAAAGTTAATTTTAAAATTAAAGGAAGAATATGAATAA", "aa": "MINKNLSKVLENSIKNNKLQQVYLLFSRANQDIDLDILEFVRLVNDEKQISWHQCLQKEYCFIIDGGQGEISKENLQEFLNRVSKSSLFANKYKFLIIKCVENITLKAANSILKFLENPPFNTIILLHTIKPQNVLKTIKSRCFIIDIRDDFNAVASVESFFITYKIDVSKQKQFRELADLIYNSPANLFNLILFYIQQLNIENYKNIIHFSIFIFKQIYELKNSIKKDKNISKFDSISILNIINNLYNFVELIDNNNCIFNIQKAKLILKLKEEYE", "aa_hexdigest": "96522f294848a488d618bde3ae1760d8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158B3F63", "ncbi_nrp_id": "WP_175933398.1", "uniref100_id": "UniRef100_UPI00158B3F63", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B3F63", "RefSeq:WP_175933398.1", "UniRef:UniRef100_UPI00158B3F63" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B3F63", "uniref90_id": "UniRef90_D1J7N6" }, "psc": { "uniref90_id": "UniRef90_D1J7N6", "gene": "DnaX", "product": "DNA polymerase III subunit", "uniref50_id": "UniRef50_D1J7N6", "cog_id": "COG2812", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_D1J7N6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N6" ], "product": "DNA polymerase III subunit" }, "genes": [ "dnaX" ], "id": "AHCKFKLKGB_247", "locus": "GCA013367975_00212" }, { "type": "cds", "sequence": "contig_1", "start": 118626, "stop": 119339, "strand": "+", "frame": 3, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0008649", "GO:0008757", "KEGG:K07056", "RefSeq:WP_036439211.1", "SO:0001217", "UniParc:UPI000512999F", "UniRef:UniRef100_A0A454C981", "UniRef:UniRef50_D1J7N7", "UniRef:UniRef90_D1J7N7" ], "nt": "ATGAATAATAAAATTTATATAGTTGGAACTCCAATTGGAAATTTAGAAGATATAACATATAGAGCAATTAAGGTTTTACAATCCAGCGATATAATTTTGTGTGAAGATATAAGAGTTAGTCAAAAATTATTGCAACATTACGAAATTAATAACAAACGCCTAATTGCATACCATAAATTCAATGAAAAAGAAATGACGCCTAAAATTATTGAATTATTGCAACAGGGGATGATTATTTCATTGATTTCTGATGCCGGCATGCCTTGCATTTCTGATCCTGGTTTTGAAGTTATTAAGGCAGCCAAGGCAGCAAATATTGGCATTGATGTAATTGGAGGCCCAACTGCTCTGATACATGCAATAATAAAGGCTAATTTGGATAATTGCTTCACATTTCTAGGCTTTTTAAAAGATAAGTCAGGTGCAAGACAAAATCAATTAAAATCTTTGACATTTGGCACATATGTTGCTTACGTTTCACCTCATAAATTGCTTTCAACTATTAAAGATTTTGAGATTATTTTTCAAGAGCAAGTTAAATTATTTTTAATTAAAGAAATGACAAAAATGTTTGAAACCTCATTTGAAGGAACACCTCAAGAAGTTCTAATGCAACTAAGCGAAGAAGATCAACGCGGTGAATATACTCTTGTATTTAGTTTCAAAGAACCAAAGAAACTTAAAATTAACAAATATAAAAAAGAAATAAAATAA", "aa": "MNNKIYIVGTPIGNLEDITYRAIKVLQSSDIILCEDIRVSQKLLQHYEINNKRLIAYHKFNEKEMTPKIIELLQQGMIISLISDAGMPCISDPGFEVIKAAKAANIGIDVIGGPTALIHAIIKANLDNCFTFLGFLKDKSGARQNQLKSLTFGTYVAYVSPHKLLSTIKDFEIIFQEQVKLFLIKEMTKMFETSFEGTPQEVLMQLSEEDQRGEYTLVFSFKEPKKLKINKYKKEIK", "aa_hexdigest": "ff0c5a3f7d186d0d3329cc4eb6c74e29", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000512999F", "ncbi_nrp_id": "WP_036439211.1", "uniref100_id": "UniRef100_A0A454C981", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512999F", "RefSeq:WP_036439211.1", "UniRef:UniRef100_A0A454C981" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C981", "uniref90_id": "UniRef90_D1J7N7" }, "psc": { "uniref90_id": "UniRef90_D1J7N7", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_D1J7N7", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0008649", "GO:0008757" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N7" ], "product": "Ribosomal RNA small subunit methyltransferase I" }, "genes": [ "rsmI" ], "id": "AHCKFKLKGB_248", "locus": "GCA013367975_00213" }, { "type": "cds", "sequence": "contig_1", "start": 119383, "stop": 119646, "strand": "-", "frame": 1, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02968", "RefSeq:WP_012855378.1", "SO:0001217", "UniParc:UPI0001BC20B1", "UniRef:UniRef100_D1J7N8", "UniRef:UniRef50_Q98PR3", "UniRef:UniRef90_D1J7N8" ], "nt": "ATGGCAAACATTAAATCAAAACAAAAAGCAATATTATCTAACGAAAAAGCAAACATCCGTAATTCAGCCGTTAAATCATCAGTTAAAACAGCTATTAAGAAAGCAAAAATTGCTGCTGTTAATAAAGATCCTAAAGCTGCTGAATTTCAAGCTAATGCTCACCATGAAATTGACAAAGCTGTTTCAAAGGGCGTTTTACATCAAAACAATGGTTCTAGAAAAGCTAGCCGTTTAGATGCATTTATTGCTAAAAATAACAACTAA", "aa": "MANIKSKQKAILSNEKANIRNSAVKSSVKTAIKKAKIAAVNKDPKAAEFQANAHHEIDKAVSKGVLHQNNGSRKASRLDAFIAKNNN", "aa_hexdigest": "19721fc9eea4c2bbc137fa79a30a1504", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC20B1", "ncbi_nrp_id": "WP_012855378.1", "uniref100_id": "UniRef100_D1J7N8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20B1", "RefSeq:WP_012855378.1", "UniRef:UniRef100_D1J7N8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7N8", "uniref90_id": "UniRef90_D1J7N8" }, "psc": { "uniref90_id": "UniRef90_D1J7N8", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_Q98PR3", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PR3" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "AHCKFKLKGB_249", "locus": "GCA013367975_00214" }, { "type": "cds", "sequence": "contig_1", "start": 119740, "stop": 120015, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAACAAAGAAAAAATATGATGGATATCTTAAATATTCAAAAAATAATGAATCTGAGTTGATAAATTATTATCTAACTAATAATAAAAATATCTATTTAAAGTTGAACAAGGAACAAGAAATCGTAGCCTATGTGCCACGTAAGTATATGAATACAATAATATTGGATAATTTTTTAAAATCATCAGTTCCTAAACTTCTAGAAATAAAAACAAAAATTGAGCAAAAGAAAATACTTGCAATTGATATAGAAAAAAATAAATTTTATTTATGA", "aa": "MVTKKKYDGYLKYSKNNESELINYYLTNNKNIYLKLNKEQEIVAYVPRKYMNTIILDNFLKSSVPKLLEIKTKIEQKKILAIDIEKNKFYL", "aa_hexdigest": "56f9b88474e4b9eb27b636cdc7c7c8a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10943.76899999999, "isoelectric_point": 9.566286277770995 }, "id": "AHCKFKLKGB_250", "locus": "GCA013367975_00215" }, { "type": "cds", "sequence": "contig_1", "start": 120457, "stop": 120798, "strand": "+", "frame": 1, "gene": null, "product": "NERD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7P0", "UniRef:UniRef90_D1J7P0" ], "nt": "ATGGAAAATAAAACAAAATTTATTAATGGAATTATTATAGGATTAGTTTTGCTATTAGTAATAGTTATAATTCTAATTTCAGTTTCAATTGTTCTTTCTAAAAAGAATAGAAGATTTTTAAAAGATCAAAAGGGATTTTTAGATTTATACAATACACAATTAAAAAATATTAGTGAATCTATAAAAAATTGCAAATTTATAGGTGCTAACGATTATTCTTATAACGATCAAAGAATACACTTAGATGATATATTTGTTACTGATAAAATTATTTTTATATTAAAGCCTTATATGTATGACTCCGACTCTTCTATTCAAGGTGACTATTTTAGCAACGAATGA", "aa": "MENKTKFINGIIIGLVLLLVIVIILISVSIVLSKKNRRFLKDQKGFLDLYNTQLKNISESIKNCKFIGANDYSYNDQRIHLDDIFVTDKIIFILKPYMYDSDSSIQGDYFSNE", "aa_hexdigest": "3c37016237e60afb46f28d9e8afd259c", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 120457, "stop": 121095, "observations": { "mutation": [ 120796 ], "stop": [ 120796 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P0", "query_cov": 1.0, "subject_cov": 0.5330188679245284, "identity": 0.991, "score": 197.0, "evalue": 4.08e-62, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MENKTKFINGIIIGLVLLLVIVIILISVSIVLSKKNRRFLKDQKGFLDSYNTQLKNISESIKNCKFIGANDYSYNDQRIHLDDIFVTDKIIFILKPYMYDSDSSIQGDYFSNEWILTKSNGKKSKIPNQLISLKKSMNNFGLLFSNEMSICSIFVHNLSTELNIEDLPSFQGIYNVKDLCASIKSVYNSNINSMYNQSVSTIFNVLNNKIAK" }, "paralog": false, "identity": 0.9811320754716981, "score": 362.0, "evalue": 1e-129, "description": "Internal stop codon at 120796. Nonsense mutation around 120796.", "psc": { "uniref90_id": "UniRef90_D1J7P0", "product": "NERD domain-containing protein", "uniref50_id": "UniRef50_D1J7P0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P0", "UniRef:UniRef50_D1J7P0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7P0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7P0" ], "product": "NERD domain-containing protein" } }, "id": "AHCKFKLKGB_251", "locus": "GCA013367975_00216" }, { "type": "cds", "sequence": "contig_1", "start": 120865, "stop": 121095, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTTGGACTATTGTTTTCTAATGAAATGAGTATATGTTCAATTTTTGTACATAATTTAAGCACGGAACTAAATATAGAAGATTTGCCATCGTTTCAAGGAATTTGCAATGTTAAAGATTTGTGTGCTTCAATAAAATCTGTATATAATTCGAATATTAATTCAATGTATAACCAATCTGTTTCAACTATTTTCAATGTTTTAAATAATAAAATTGCAAAATAA", "aa": "MNNFGLLFSNEMSICSIFVHNLSTELNIEDLPSFQGICNVKDLCASIKSVYNSNINSMYNQSVSTIFNVLNNKIAK", "aa_hexdigest": "0e9bf6c979980a3b50b64596d209f8ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8496.635599999994, "isoelectric_point": 5.537618446350096 }, "id": "AHCKFKLKGB_252", "locus": "GCA013367975_00217" }, { "type": "cds", "sequence": "contig_1", "start": 121255, "stop": 121443, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAGAACAAAAAACAGTATTGGAAAAATACTATATTTCTGAAGCTTTTGATAAAGATAGAAATATTTCATTTAACTTTAAAAAGGCAAATAAGGGCATTTCAGGAAACTTTTTAAATTTTTCTAGCGCAGTTGAAGAATTTATTAAAGTTAGTGAAAATACACAAAACCCAACCAAATGTTGA", "aa": "MAKEQKTVLEKYYISEAFDKDRNISFNFKKANKGISGNFLNFSSAVEEFIKVSENTQNPTKC", "aa_hexdigest": "c6147096031e44b0c32b36152fe9cf71", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7128.958700000002, "isoelectric_point": 8.787637138366701 }, "id": "AHCKFKLKGB_253", "locus": "GCA013367975_00218" }, { "type": "cds", "sequence": "contig_1", "start": 122193, "stop": 122480, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGCGTGAAGTTTTAGGTGAATTAAATATTAAATATAAAGAATTTATTGGTGAAGCGGCATTTTATGGACCTAAGGTTGATTTTCAAGTAAAAACTGCACTAAATAGAATTATTACTATGTCAACCTTACAATTAGACTTCTTATTGCCTTCAAGGTTCGAAATGAAATTTGTTGATAGCAATGAACAATTTGTAACACCAGTATTAATTCATAGAGGTTTAATTGGAACTTATGAAAGATTTATTGCAACACTACTTGAACAAACAAAAGGTGTATTGCCATTTTGA", "aa": "MREVLGELNIKYKEFIGEAAFYGPKVDFQVKTALNRIITMSTLQLDFLLPSRFEMKFVDSNEQFVTPVLIHRGLIGTYERFIATLLEQTKGVLPF", "aa_hexdigest": "f194bf682ba591db6ef0c59e7468d337", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0029CA135A", "query_cov": 0.8736842105263158, "subject_cov": 0.8217821782178217, "identity": 0.578, "score": 102.0, "evalue": 1.71e-26, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.3" ], "uniref50_id": "UniRef50_Q71HR4", "kegg_orthology_id": "K01868" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 78, "aa_cov": 0.7894736842105263, "hmm_cov": 0.9743589743589743, "evalue": 9.476259504864406e-15, "score": 50.41576385498047, "start": 14, "stop": 88 } ], "seq_stats": { "molecular_weight": 10973.738299999988, "isoelectric_point": 6.570439720153809 }, "id": "AHCKFKLKGB_254", "locus": "GCA013367975_00219" }, { "type": "cds", "sequence": "contig_1", "start": 122499, "stop": 122867, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGTTTGCCCCAATTAAAATGGATAACGAAACATTAGTTGCTAGACCTATGACTTGCCCACACCATATAATATTGTTCAATTCAACAAGACGTTCATACAAAGACTTGCCAATTAGATATTCAGAACAATCAAGGTTATATCGTTACGAAGCATCGGGTGCTTTAAGCGGACTTGAAAGAGTTAGAGGAATGGATCTAACAGAAGGTCACATTTTTGTTAGACCAGATCAAATAAGATCAGAATTTAAGCACTTGTATAAAATGATATTACAAGCATTGAAAGATTTTAATATCGAAATAGACCATATTTCTTTATCATTAAGAGATCCACAAGATACTCAAAAATTCTTCCAAAATGATGAAATGTGA", "aa": "MFAPIKMDNETLVARPMTCPHHIILFNSTRRSYKDLPIRYSEQSRLYRYEASGALSGLERVRGMDLTEGHIFVRPDQIRSEFKHLYKMILQALKDFNIEIDHISLSLRDPQDTQKFFQNDEM", "aa_hexdigest": "7a07e828130cfa861d1bd06ca1ae0583", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 104, "aa_cov": 0.8360655737704918, "hmm_cov": 0.9807692307692307, "evalue": 7.503003831319763e-22, "score": 73.55043029785156, "start": 8, "stop": 109 } ], "seq_stats": { "molecular_weight": 14463.435299999981, "isoelectric_point": 6.968255805969237 }, "id": "AHCKFKLKGB_255", "locus": "GCA013367975_00220" }, { "type": "cds", "sequence": "contig_1", "start": 122886, "stop": 123005, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTCATAATGCAATTAGAGATTATGTTTTAAAACTTGATAAAAAATTTGGTTTTAGAGAAGTACTAACTCCACACTTTGGCGAAAAGAAGCTTTATGAAATAAGTGGACACTGA", "aa": "MKIHNAIRDYVLKLDKKFGFREVLTPHFGEKKLYEISGH", "aa_hexdigest": "a17315a2b28feeeb87eb514c8fb89092", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4659.415, "isoelectric_point": 9.5220609664917 }, "id": "AHCKFKLKGB_256", "locus": "GCA013367975_00221" }, { "type": "cds", "sequence": "contig_1", "start": 123018, "stop": 123179, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACAAGAATCTATGGTACTTGTTGGGAAACAAAAGATGAATTAGCAAAATATCTAGATATCTTAAGGGAAAGAAAAGAAAGAGATCATCGTAAAATAGGAAGAGATTTAAATTTATTCACTTTTAACCAATTAGGTGGACAAGGATTCCCATTTTGA", "aa": "MLTRIYGTCWETKDELAKYLDILRERKERDHRKIGRDLNLFTFNQLGGQGFPF", "aa_hexdigest": "c58cc23b31d45c62610ae0c87358d88e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6391.2588, "isoelectric_point": 8.976658439636232 }, "id": "AHCKFKLKGB_257", "locus": "GCA013367975_00222" }, { "type": "cds", "sequence": "contig_1", "start": 123312, "stop": 123629, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCAAATAAAAAATTAAATCATTCAGCAAGCCATTTATTAGCGGCTGCTATATTAAAACTTTATCCAAATGCTAAATTGGCAATTGGACCAGCCATTGAAGAAGGGTTCTATTACGACTTTGAATTTGAAAATCCAATTTTAGAATCAGATCTTCCAAATATTGAAAAAGTAATGAAAGAATTAGCAAAAGAAGGTTACGAAATGCGAAAAGTTGGCATTGAACAATATTCTTTTGAAAATCAACCATATAAAAAAGAACTTTACGAAGAATTTAAAAAAGAAGGAAAAGAAATAACATTCTTTCAATATTGA", "aa": "MEANKKLNHSASHLLAAAILKLYPNAKLAIGPAIEEGFYYDFEFENPILESDLPNIEKVMKELAKEGYEMRKVGIEQYSFENQPYKKELYEEFKKEGKEITFFQY", "aa_hexdigest": "5b3a71cd39b162d39bb0cc2e71f1f051", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12319.913599999993, "isoelectric_point": 5.108199882507324 }, "id": "AHCKFKLKGB_258", "locus": "GCA013367975_00223" }, { "type": "cds", "sequence": "contig_1", "start": 123629, "stop": 124303, "strand": "-", "frame": 3, "gene": null, "product": "Tryptophan--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "nt": "ATGACGCAATTTAAAGATAAATCAGCTAAAATAAAAACTGCAAATGGTATGGAAACAATTCCAACAGGAATTTTAATGTATCCTACCTTAATGGTTGGCGATATATTACTATATAATGCAGATATTATTCCTGTTGGCATTGACCAAAAGCAACATGTTGAATTGACTAGAAATTTAGCAATTAGATTAAATAATAAATTCAATACAACGTACAAAATACCTGAACCATATATACCAGAAGTTGGGGCCAAAATAATGTCTTTAACTGACCCAAGTAAGAAAATGTCAAAATCTGATACAAATCCTAAATCAGCAATTTATTTATTAGATGATCCAGAAGATGCATACAAAAAAATATTAAAAGCTGTTACAGATTCAGAAGGAAAAATATATTTGTCAGATGAAAAGCCGGGCATAAAAAACCTTTTGACTATATATAGTGCTTTAAATGATATTTCATTACAAGAAAGCGCTGAAAAATTTAAAAACAGCAATTACGGTGAATTTAAAAATGCTGTCGCTCAATGTGTAAAGGAATTTTTAATCAAGATTCAAGATAAGTATAAAAATGCCTTAAATCAAATTGAAACATTGAGCAAAGAGGGAGCAAAAAAGGCAAGTGAAGTTGCCCAAATTAACTTAGATATGTTATTAAAAGGAATAGGATTAAGATAA", "aa": "MTQFKDKSAKIKTANGMETIPTGILMYPTLMVGDILLYNADIIPVGIDQKQHVELTRNLAIRLNNKFNTTYKIPEPYIPEVGAKIMSLTDPSKKMSKSDTNPKSAIYLLDDPEDAYKKILKAVTDSEGKIYLSDEKPGIKNLLTIYSALNDISLQESAEKFKNSNYGEFKNAVAQCVKEFLIKIQDKYKNALNQIETLSKEGAKKASEVAQINLDMLLKGIGLR", "aa_hexdigest": "fd7b8c8172c37627a113216ad05a7c0b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3B0PAD4", "query_cov": 1.0, "subject_cov": 0.9333333333333333, "identity": 0.71, "score": 323.0, "evalue": 1.59e-109, "valid": false, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_A0A3B0PAD4", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PAD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "product": "Tryptophan--tRNA ligase" }, "genes": [], "id": "AHCKFKLKGB_259", "locus": "GCA013367975_00224" }, { "type": "cds", "sequence": "contig_1", "start": 124343, "stop": 124630, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATTCTGAAAAGAAGACTTTATTAAGTGGAATTACTGCAACTGGTAAATTAACGCTAGCAAATTATATTGGCGCTATAAAAAATATGGTTAAATTGCAAGAAGAATATAACAATTACATTTTTATAGCAGATTTACATGCTCTAACGTTGCCAATTGATCCTAAGCAATTATCAAAGAATAAAAAGGATATTTTTGCACTTTATATTGCATGTGGAATTGACCCTAAAAAGAGTGTGTTATTTTTTCAATCAGATGTCATTCAACACGGATTAATGAATTGA", "aa": "MQNSEKKTLLSGITATGKLTLANYIGAIKNMVKLQEEYNNYIFIADLHALTLPIDPKQLSKNKKDIFALYIACGIDPKKSVLFFQSDVIQHGLMN", "aa_hexdigest": "a613fab4aa3aa15dabfc15552bb97150", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10644.364899999991, "isoelectric_point": 9.041578102111817 }, "id": "AHCKFKLKGB_260", "locus": "GCA013367975_00225" }, { "type": "cds", "sequence": "contig_1", "start": 124949, "stop": 125185, "strand": "+", "frame": 2, "gene": "ptsG2", "product": "PTS EIIB type-1 domain-containing protein", "db_xrefs": [ "COG:COG1264", "COG:G", "SO:0001217", "UniRef:UniRef50_N9UZL6", "UniRef:UniRef90_D1J7P4" ], "nt": "ATGCAAGATTTAATTTTGTATTTGGGTGGAATTTCAAATATAGTTTCTTCATCAGCAACTCAAAAAATTGTAAGAATTGCATTTAAAAATAAAACAATTATTTCTATTGAAAAAATAAAAAAACTTAATGGCATATCAGGTATTGCATTTCAATCTTCAAATATATCATTAGTTGTTGGAAATTGTGCAAATTATTTATCTACATTACTTAATAAGGAATTAAACAATCATGAATAA", "aa": "MQDLILYLGGISNIVSSSATQKIVRIAFKNKTIISIEKIKKLNGISGIAFQSSNISLVVGNCANYLSTLLNKELNNHE", "aa_hexdigest": "5c8a37d1dd235bc9fa8ae10e3c227502", "start_type": "ATG", "rbs_motif": null, "genes": [ "ptsG2" ], "truncated": "5-prime", "pseudogene": { "start": 124774, "stop": 125185, "observations": { "insertion": [ 124807 ], "stop": [ 124880 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P4", "query_cov": 1.0, "subject_cov": 0.5735294117647058, "identity": 0.9740000000000001, "score": 142.0, "evalue": 2.53e-42, "gene_start": 1, "gene_end": 78, "reference_start": 59, "reference_end": 136, "reference_sequence": "ISYIGKTIIGEKMLNKNKFLYIFLIIITLGLVLIFWRKKYKILKTKNYLTTDNEITFSMQDLILYLGGISNIVSSSATQKIVRISFKNKTNISIEKIKKLNGISGIAFQSSNISLVVGNCANYLSTLLNKELNNHE" }, "paralog": false, "identity": 0.9485294117647058, "score": 244.0, "evalue": 9.26e-85, "description": "Internal stop codon at 124880. Frameshift due to insertion around 124807.", "psc": { "uniref90_id": "UniRef90_D1J7P4", "gene": "PtsG2", "product": "PTS EIIB type-1 domain-containing protein", "uniref50_id": "UniRef50_N9UZL6", "cog_id": "COG1264", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P4", "UniRef:UniRef50_N9UZL6", "COG:COG1264", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_N9UZL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UZL6" ], "product": "PTS EIIB type-1 domain-containing protein" } }, "id": "AHCKFKLKGB_261", "locus": "GCA013367975_00226" }, { "type": "cds", "sequence": "contig_1", "start": 125178, "stop": 125552, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114FF0", "UniRef:UniRef90_UPI0024114FF0" ], "nt": "ATGAATAACAAAGATTCTAAATATCAAACATTAATGGCTAATTTATTGAATATTGATACATATGATACTTCACTATATACATCTATAAATAGTGAAAATTTTTTTGATATTTATAACAATTTTGGCCAAGAAATATTTGATCAAATTTTCAAAAATTACAAATTTAATTGTATTTTAACCGAAGTCAAAAATAAAGAAATGCGCCAATTGCTAGAAAAGGCACAAAATAAGCAGGAAATAATAACAATTTATAAAGATAATAACGTCAGAATCGAACAATCGATATTCAAGATGCTATCAACTGATTTTGAAAAGGCTAAAACTTTGTTAATTACTAAATTAGAATCAGAATTTCAAAAATCAATAGTAAATTGA", "aa": "MNNKDSKYQTLMANLLNIDTYDTSLYTSINSENFFDIYNNFGQEIFDQIFKNYKFNCILTEVKNKEMRQLLEKAQNKQEIITIYKDNNVRIEQSIFKMLSTDFEKAKTLLITKLESEFQKSIVN", "aa_hexdigest": "11dc00f925aeb645eb72db61d3602459", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0024114FF0", "query_cov": 1.0, "subject_cov": 0.8157894736842105, "identity": 1.0, "score": 153.0, "evalue": 1.46e-45, "valid": true, "uniref50_id": "UniRef50_UPI0024114FF0" }, "pscc": { "uniref50_id": "UniRef50_UPI0024114FF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114FF0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14779.667499999985, "isoelectric_point": 5.516190147399901 }, "id": "AHCKFKLKGB_262", "locus": "GCA013367975_00227" }, { "type": "cds", "sequence": "contig_1", "start": 125607, "stop": 125855, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATGTAGGTTTTTTATTTATTTCAATAAAAACGGATAAAAAGACAATCTTTGCTCCCTTATTATTTAAAGAAGTAACATTAGAAGTTAAAAATTCCTTGGTTTATTTGAAATCAAATTCAGATGTAAGAATAAATAGTAAGTTAGTAACATTTTTATCTCAAGAAGGTTTTTTGTTAAATATTGATAATTTAGATTTTTCTAATTTGTCAATTGAATCTATATTTGAGCATTTAAAAAGAATTTGA", "aa": "MHVGFLFISIKTDKKTIFAPLLFKEVTLEVKNSLVYLKSNSDVRINSKLVTFLSQEGFLLNIDNLDFSNLSIESIFEHLKRI", "aa_hexdigest": "470694e780c225f6cfea60ea59c7d5ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9461.970299999994, "isoelectric_point": 8.050699043273926 }, "id": "AHCKFKLKGB_263", "locus": "GCA013367975_00228" }, { "type": "cds", "sequence": "contig_1", "start": 125871, "stop": 125993, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCAATCACTAAGAAGCAATATTCCGCTTGTATCTCAAAGTGATATTCAAAACGTATCTATCGAATTTCATCCCGGAATGATTTTTGGATTTTATAACGTTTCTTCTGGATATTTATGA", "aa": "MPQSLRSNIPLVSQSDIQNVSIEFHPGMIFGFYNVSSGYL", "aa_hexdigest": "3b3542bb815f6037943a18df730e216e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4474.0337, "isoelectric_point": 5.3026460647583 }, "id": "AHCKFKLKGB_264", "locus": "GCA013367975_00229" }, { "type": "cds", "sequence": "contig_1", "start": 126000, "stop": 126188, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTATTGAAAACGATGAATTTGAAAGTATTTTAGTACCCGAAATTAATAAAAATGTTTATAGAGAAAAAATTAATACAAGTATTTTTAATAATAAATTTAAGTTATTTAAGGTACAAAAAACAAACTTTTCACAAGATGTTGCTACAGTGTCTGCACTATGTCAAGATACAATAATTTGA", "aa": "MKKIIENDEFESILVPEINKNVYREKINTSIFNNKFKLFKVQKTNFSQDVATVSALCQDTII", "aa_hexdigest": "82aa51f4507ea3369e6fa1a6adb94bd3", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7239.282900000003, "isoelectric_point": 7.844142341613771 }, "id": "AHCKFKLKGB_265", "locus": "GCA013367975_00230" }, { "type": "cds", "sequence": "contig_1", "start": 126270, "stop": 127088, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTCAAAAGAAGGCCGCCCTTGATGTATTGAGAAATAGACTAGGACAATTGAGCTTATTTTGTTTATTTGCTTTAAATGATAAGAATTTAAGACAAGAGGTATTTTACGAACCATTAAAAAAATTTATAGAAAAAATTGAGAATTTTTCTATTTCTCAAGTTGAAAAAGGAATTCAAATTTTTTCTGATGAAGATAAAGGTTATGTTGATACATTGAATGAAATAGAAGCATTGCCTAACTTAAATAACTTACTTCATTTCTATGCCTCAACATTGAATGCCAATCTAACAATAAATGATTTTAATACCCTAAGATTATTGGCAAAAGATATAAAATATGATGTTGAAGGACATACTTTTAATGATAAGAAAGAACTTAAAAAATATCTATATGAAGTAAATTTAAAAAGAAGACCGTCAATATTTTCAATTTATCCAAGGTCAATAAAAGAAGCTAGTGAATTAATTATTAATGCGCCTGAATTATTTAAAATCAATGTAGATGAAGCAGTAAAATACATTGATAAAGTAGAGTTTGCCGAATTGGAAACTTTTGTTGAAAAATATAAAAATTTATTGATTAAAAAAACAATAAATGTAAGTAACTCAGCCGTTTTATCTAATATGTTATTTGCTAAATTAATAAATACAATTTCGAATTTCAATGATGAACAAAAACGTGAATATACATCATTTGCAATGTCTATAAGAACAGGTAGCTTAAAGCCATATATGTTTTTCCATAGATATAAAAACATAATTAAAGTATTATTTCCAATAATTGTAACTACTCCTGAATTAGATTTGTCAATGTGA", "aa": "MSQKKAALDVLRNRLGQLSLFCLFALNDKNLRQEVFYEPLKKFIEKIENFSISQVEKGIQIFSDEDKGYVDTLNEIEALPNLNNLLHFYASTLNANLTINDFNTLRLLAKDIKYDVEGHTFNDKKELKKYLYEVNLKRRPSIFSIYPRSIKEASELIINAPELFKINVDEAVKYIDKVEFAELETFVEKYKNLLIKKTINVSNSAVLSNMLFAKLINTISNFNDEQKREYTSFAMSIRTGSLKPYMFFHRYKNIIKVLFPIIVTTPELDLSM", "aa_hexdigest": "87c00da7256fcdfefac955d39ce4ccd3", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31726.381600000037, "isoelectric_point": 8.651479911804202 }, "id": "AHCKFKLKGB_266", "locus": "GCA013367975_00231" }, { "type": "cds", "sequence": "contig_1", "start": 127260, "stop": 127469, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGATATTGAATCTCTTCTAGATTACGCTACTGCTAGAGGAACTTATTCAATATTGCTTGATAAGAACTATCGTTCAAAGAGGGCATCATTAATGACATTCAGTTCTAAACATTTTTATGATTCCAAATTAGATGTAATAGATGATTTTAAATTAGCATTTTCTAGTGAAAAACCAATTGATGTAATTCAAGTTGATGGTGAATGA", "aa": "MGDIESLLDYATARGTYSILLDKNYRSKRASLMTFSSKHFYDSKLDVIDDFKLAFSSEKPIDVIQVDGE", "aa_hexdigest": "553a809e908339a412a86b6e93f2c402", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7856.7582, "isoelectric_point": 4.954848289489745 }, "id": "AHCKFKLKGB_267", "locus": "GCA013367975_00232" }, { "type": "cds", "sequence": "contig_1", "start": 127584, "stop": 128267, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "TTGACAAATGCAATATTTAGCGTTGAACCAGAACTAGAAAAAGCCTTATTTTCCGATAAAATAACAATAAAAAATATTGAAAACATTCAAGGTGATGAAGCTGATTTAGTCATTATGTCTGTTGTTTATGACAAAAAAACAGCATTGTTTGGAACATACGTTGCAAGGAAGGGTGGAAAAAACGCCCTAAATGTTGCCATATCAAGAGCAAGAGAAAAAATTATTGTTGTAAAATCAATTTATGCAGATGATGTTGAAATTAATGAACGTTCAACATCTGATATGCGTTTATTCAAAGAATGGCTAAAATTTTTAGATTTATCAATAATTGAACAAAAAAACTATTTATTTTTAGATAATGAAAATAAAAAATATGATGAAAAAACACAAACAATATTGTTACCAAAAGTAAGCAACCTTCAAATGGATGTATTGGCAAAATTAAACGAATTATTGAAGGAACTAAATGCTAACAATTTCGAAATAATTTCGAATTATTCAATAGGAACTAAATTATTAGACTTAGTCTTAATCAATAAAATTTCAAATAATATGATTATTGGTATCAATATTGACGATTTTAAATATTTGCCTGATTATAGAAAATATTTAAAATTTAAAGATGATTTTAATTTTTTACTATCAAAATCATATCCAATAATTAATATTTCTAGAATAAGTTGA", "aa": "MTNAIFSVEPELEKALFSDKITIKNIENIQGDEADLVIMSVVYDKKTALFGTYVARKGGKNALNVAISRAREKIIVVKSIYADDVEINERSTSDMRLFKEWLKFLDLSIIEQKNYLFLDNENKKYDEKTQTILLPKVSNLQMDVLAKLNELLKELNANNFEIISNYSIGTKLLDLVLINKISNNMIIGINIDDFKYLPDYRKYLKFKDDFNFLLSKSYPIINISRIS", "aa_hexdigest": "62ad1e8a35138bd2bde679c02ac50b6d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 64, "aa_cov": 0.2775330396475771, "hmm_cov": 0.9375, "evalue": 5.460297205870526e-12, "score": 40.96646499633789, "start": 15, "stop": 77 } ], "seq_stats": { "molecular_weight": 26225.099500000044, "isoelectric_point": 6.436299705505371 }, "id": "AHCKFKLKGB_268", "locus": "GCA013367975_00233" }, { "type": "cds", "sequence": "contig_1", "start": 128346, "stop": 129395, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9F07", "UniRef:UniRef90_UPI00215D9F07" ], "nt": "TTGCCCGAAGAAAATAACAATTTAAATGTAGAAGAAATTAAAGAAAACATTATAGAAAATAATGCCTTAAAAAACATAAAATCAAGCCAAATTAAACAAGGTCCTGAATTAAAAGAAATAGATTTTGAAAATGTTGATGACGAAACTTTTGAAGTTGATCAAGATTCTAATAATAAAATTTATGAATTAAATGAATCTGAGGTTGCTCAATTTGAAGAATTCCATAATAAGAATATAAATCATGAATTTAATGACCAAACCGAATCAAGAGCCGAATACTCAAAGGACTATGAAAAAGATTTTTTGATTAATTATAATGATGTTGAATTAGATAATACGCATATTGATCAGGAATTTCACATTGAAAATGAATTTGAATCAACAATAAATAATAGTAATGCGGTAAAGATTGAAGATGCAACTTCATCATCGGCTAACGATGAATTAAAAATAATTAATTCTAATGAACAAAAAATTTCTCCAAAAACAGCAAGTGAAGATATTGAATTGGGATTTTCAATTGAAGATATAAACAAAGAAAATATTAGCAATTTATTTTCTAATACATTTGATGATCTTGATATAAATATAGCACCTGAAAGTGAACAACAAGTTGAGGCTAAACTTAATTATGATTCAGGCATTATTCAAGATAAGTTGCATGAGCAAAACGATGAATATTTTAATTTAGAAGAAAATAATATTGAAGAGCAACCAAAAAATAATAATGTTCAAAGCGCAGATGAGACATTAAAATCTCAATTAGTTTATATTGACAATAATAAAGAAGTAAACGATAAACAAAAAAGCTATGATTTTGCAGGTTTATTTGCTGACGAAAATGAAGAAACTTCTACAGATGTAAAAAACATGATAGACATGAATACTGCAGAATATAATCAATATATGAAAAATAAAGATATTGAACAAGATGAACAAAATAAAGAGATAACTAAAAATACTGCTGATGAAGAAATATTGAATTTTGATTTAATCGAAGATGAAAAAACTACGGAATTCAATGTGGAGGACAACAATGTCAAAAAATAG", "aa": "MPEENNNLNVEEIKENIIENNALKNIKSSQIKQGPELKEIDFENVDDETFEVDQDSNNKIYELNESEVAQFEEFHNKNINHEFNDQTESRAEYSKDYEKDFLINYNDVELDNTHIDQEFHIENEFESTINNSNAVKIEDATSSSANDELKIINSNEQKISPKTASEDIELGFSIEDINKENISNLFSNTFDDLDINIAPESEQQVEAKLNYDSGIIQDKLHEQNDEYFNLEENNIEEQPKNNNVQSADETLKSQLVYIDNNKEVNDKQKSYDFAGLFADENEETSTDVKNMIDMNTAEYNQYMKNKDIEQDEQNKEITKNTADEEILNFDLIEDEKTTEFNVEDNNVKK", "aa_hexdigest": "b5251320463b405efb9e97872498026b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 128046, "stop": 129395, "observations": { "mutation": [ 128265 ], "stop": [ 128265 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D9F07", "query_cov": 1.0, "subject_cov": 0.6856581532416502, "identity": 0.9540000000000001, "score": 613.0, "evalue": 8.23e-218, "gene_start": 1, "gene_end": 349, "reference_start": 161, "reference_end": 509, "reference_sequence": "MRLFKEWLKFLDLSIIEQKNYLFLDNENKKYDEKTQTILLPKVSNLQMAVLAKLNELLKELNANNFEIISNYSIGTKLLDLVLINKISNNMIIGINIDDFKYLPDYRKYLKFKDDFNFLLSKSYPIINISRISWIVENKQILNKIKILIKHEEELNSQILLSEENNNLNVEEIKENIIENNAIKNIKSNQIKQSPELKEIDFENVDDETFEVDQDSNNKIYELNESEVAQFEEFHNKNINHEFNDQTESRAEYSKDYEKDFLINYNDIELDNTHIDQEFHIENEFESTINNSNAVKIEDATSSSANDELKITNSNEQKISAKTASEDIESGFSIEDINKENISNLFSNTFDDLDINMALESEQQVEAKLNYDSGIVQDKLHEQNDEYFNLEENDIEEQQKNNDVQSPDETLKSQLVYIDNNKEVNDKQKSYDFAGLFADENEETSTDVKNMIDMNTAEYNQYMKNKDIEQDEQNKEITKNTADEEILNFDLIEDEKTTEFNVEDNNVKK" }, "paralog": false, "identity": 0.9643652561247216, "score": 856.0, "evalue": 2.68e-316, "description": "Internal stop codon at 128265. Nonsense mutation around 128265.", "psc": { "uniref90_id": "UniRef90_UPI00215D9F07", "uniref50_id": "UniRef50_UPI00215D9F07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D9F07", "UniRef:UniRef50_UPI00215D9F07" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9F07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9F07" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 40646.94360000006, "isoelectric_point": 4.097602653503418 }, "id": "AHCKFKLKGB_269", "locus": "GCA013367975_00234" }, { "type": "cds", "sequence": "contig_1", "start": 129457, "stop": 129708, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACAAAGATATTGGAACTAAAATTGCAAAGCAAAAAAAGATTAAAAAAATTTTGTTCATAACTTTTTCTGTTGTTGGTATTGCTACAATCGTAGGGGCAACAATTGGAGTTACAAGCAGAAAAAAAATGGAATTAGTAACTTATGATGATTATGTTGATGTTTCTCTATATTCACAAATAAATTCTAAATTTAAAGAATTGTATCTCGATGGAAAACAACCTGACAAAAAAAATTACCCAATTACTTGA", "aa": "MHKDIGTKIAKQKKIKKILFITFSVVGIATIVGATIGVTSRKKMELVTYDDYVDVSLYSQINSKFKELYLDGKQPDKKNYPIT", "aa_hexdigest": "6d956b33ec10a6d5b821687dfb799fac", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9413.992499999995, "isoelectric_point": 9.663955879211429 }, "id": "AHCKFKLKGB_270", "locus": "GCA013367975_00235" }, { "type": "cds", "sequence": "contig_1", "start": 130162, "stop": 130575, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATAGTGATTACAATTACGTTGATAAATCGCTTTGGTTAACATCTCATCCTAAAAAAACCGATGAATCCGAATTAAATTATTTTAAGTTGTTTCAGTCAGATGTAAGAGACTGGTTTGTTCCTTTTAATTTCTTAAGTCAAGGAATTCAATTGAAAAAGATTGAAAAAAATGAAAATAATATTTTTACTAAGACGCTCAATTTAGAATTCAAGTCAACTTTTTATACTATTGTAAAGAATAAATTAATTTTAAAAAATAAATCTAGTTATGAATATGCTATTTATGTTCCATTTTTTACAAATAAGGATAAGACCATTAAAGACGGCAAGGAAATTTATCAACCTAGAGTTTTTGAATATAGTTGTCCTATAACATTTGTTAATAGTAATTACAAAAAATTTAAATAA", "aa": "MNNSDYNYVDKSLWLTSHPKKTDESELNYFKLFQSDVRDWFVPFNFLSQGIQLKKIEKNENNIFTKTLNLEFKSTFYTIVKNKLILKNKSSYEYAIYVPFFTNKDKTIKDGKEIYQPRVFEYSCPITFVNSNYKKFK", "aa_hexdigest": "7af4551bb96884f13918d079a047cb27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16513.652399999984, "isoelectric_point": 9.333362007141115 }, "id": "AHCKFKLKGB_271", "locus": "GCA013367975_00236" }, { "type": "cds", "sequence": "contig_1", "start": 130635, "stop": 131162, "strand": "-", "frame": 2, "gene": "def", "product": "peptide deformylase", "db_xrefs": [ "COG:COG0242", "COG:J", "EC:3.5.1.88", "GO:0036211", "GO:0042586", "KEGG:K01462", "SO:0001217", "UniRef:UniRef50_Q98PN3", "UniRef:UniRef90_D1J7P7" ], "nt": "ATGTTTAAATTTGAAGATGTTAAATTAGTTCAATTACCAGAAAAAGTATTGAGAAATAAATCAAAGAACGTTGAACTGCCATTGACCGCAGAAGATGATTTATTAATACAAAAAATGATTTTTCATGTTGATGATAGTCAAACTCCTCAAACAAAATTTAGACCTGCCGTTGGAGTTGCAGCAGTTCAATATGGAATATTGAAAAATATTTTTTATGTAATGGTAAAAGACGATCAAAATAATTATATTTTTAGAGATGCTTTAATAAATCCAATAATGCTTTCACATTCAAACCATAAAATATCATTGCAAGAAGGGGAGGGATGTTTAAGTGTTAAGGAAAGCGATGCAGGTCAAGAAGGTTTTGTATATAGATATTCAAGAGTTGTAATTAAAGCATATTCATATTTTGAAAAAAAAGAAAAAATTTATGATGTTTCTGGCTATCCGGCAATTGTATTGCAACACGAATATGACCACTTACAAGGAAAATTATTTATAGACAGAATCAATAAAAATCAACCTTGA", "aa": "MFKFEDVKLVQLPEKVLRNKSKNVELPLTAEDDLLIQKMIFHVDDSQTPQTKFRPAVGVAAVQYGILKNIFYVMVKDDQNNYIFRDALINPIMLSHSNHKISLQEGEGCLSVKESDAGQEGFVYRYSRVVIKAYSYFEKKEKIYDVSGYPAIVLQHEYDHLQGKLFIDRINKNQP", "aa_hexdigest": "4356b018542dd2d3bbac879a184fc64a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7P7", "query_cov": 1.0, "subject_cov": 0.9459459459459459, "identity": 0.9940000000000001, "score": 346.0, "evalue": 2.35e-120, "valid": true, "gene": "def", "product": "peptide deformylase", "ec_ids": [ "3.5.1.88" ], "uniref50_id": "UniRef50_Q98PN3", "cog_id": "COG0242", "cog_category": "J", "kegg_orthology_id": "K01462", "go_ids": [ "GO:0036211", "GO:0042586" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN3" ], "product": "Peptide deformylase" }, "genes": [ "def" ], "id": "AHCKFKLKGB_272", "locus": "GCA013367975_00237" }, { "type": "cds", "sequence": "contig_1", "start": 131441, "stop": 131716, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGATTGAAAAAAAGCAAAAGTTCGCTAAATTAATTCAAAAACAACATTTATTTTTTAATAGAATTTTAGGGACTATTTTAATAGCTAATAATTTGGTAAATATTGCATCTGCAACATTGTTTTCATATGTTTTATCATTAACAACAATGTCTTCTGGCAAGGCTACTATTATTTCAACTGCTGTTATGACTCCACTTATTGTTTTGTTTGCAGAAATTTTACCAAAATTAATTGCAAAAGCATATCCTGAAAGAGTTATTAGATCAACGTGTTGA", "aa": "MIEKKQKFAKLIQKQHLFFNRILGTILIANNLVNIASATLFSYVLSLTTMSSGKATIISTAVMTPLIVLFAEILPKLIAKAYPERVIRSTC", "aa_hexdigest": "7766a89e40dcf13447ec1cdff86791b7", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 88, "aa_cov": 0.967032967032967, "hmm_cov": 1.0, "evalue": 7.932666993418179e-20, "score": 66.57260131835938, "start": 2, "stop": 89 } ], "seq_stats": { "molecular_weight": 10111.135399999988, "isoelectric_point": 10.17938060760498 }, "id": "AHCKFKLKGB_273", "locus": "GCA013367975_00238" }, { "type": "cds", "sequence": "contig_1", "start": 131810, "stop": 132352, "strand": "+", "frame": 2, "gene": "mpfA", "product": "Hemolysin C", "db_xrefs": [ "COG:COG1253", "COG:PT", "SO:0001217", "UniRef:UniRef50_A0A077L6Q7", "UniRef:UniRef90_D1J7P8" ], "nt": "ATGAAAAACTTGATTAATATTGCACAAGATGAAGGTGTTTTAGAAATCAACGAATCAACTATGGCAAAGAATGTTTTGGATCTTGATTCAAGCAAGGTTTCTCAAAATTACGTTAAGTTAAAAGATATTGATTATTTGAATTATAAAGCAACTTTGCAAGATGCATTAAACAAGTTTAAAGAAACTAATTATTCAAGATTGCCAATTGAAAAAGATGGAGATATGATAGGCATATTGCTTTTAAAAGATATATTCTTTTTAAAGCGTGGCAATATTATGAATTATGTTAAAACGGTGCCAAAAATTTCCGCAAATAGTTTACTTTCTATTGCATTAGAAAAATTAAGGAAGGCTAGAGCACAAATGGGTTTCGTCATTAAAAATAATAATGATGACAGTGTCATAGGAATAATTACAATCGAAGATATTATTGAAGAAGTTGTAGGCGAAATATACGACGAATATGATGATGATGAACAAATTTATGAAATAAGTTTGGAACGCTGTGAGGCCCGCGGAAACGTTAAAATTTCAACAATTTGA", "aa": "MKNLINIAQDEGVLEINESTMAKNVLDLDSSKVSQNYVKLKDIDYLNYKATLQDALNKFKETNYSRLPIEKDGDMIGILLLKDIFFLKRGNIMNYVKTVPKISANSLLSIALEKLRKARAQMGFVIKNNNDDSVIGIITIEDIIEEVVGEIYDEYDDDEQIYEISLERCEARGNVKISTI", "aa_hexdigest": "692cbb311716660370230c394aa8b159", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "mpfA" ], "truncated": "both", "pseudogene": { "start": 131510, "stop": 132559, "observations": { "mutation": [ 131714, 131735, 132350, 132365, 132422, 132548 ], "stop": [ 131714, 131735, 132350, 132365, 132422, 132548 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P8", "query_cov": 1.0, "subject_cov": 0.4147465437788018, "identity": 1.0, "score": 342.0, "evalue": 3.08e-115, "gene_start": 1, "gene_end": 180, "reference_start": 186, "reference_end": 365, "reference_sequence": "MPEPLSGNYNTLNSASGASTSTGAPWQYILYSIILFLLILFSAIFSASETAYTTVSRAKIENMIEKKQKFAKLIQKQHLFFNRILGTILIANNLVNIASATLFSYVLSLTTMSSGKATIISTAVMTPLIVLFAEILPKLIAKAYPERVIRSTCWFIEALYWIFFPITYPISKIGKKIYITNTEEDMKNLINIAQDEGVLEINESTMAKNVLDLDSSKVSQNYVKLKDIDYLNYKATLQDALNKFKETNYSRLPIEKDGDMIGILLLKDIFFLKRGNIMNYVKTVPKISANSLLSIALEKLRKARAQMGFVIKNNNDDSVIGIITIEDIIEEVVGEIYDEYDDDEQIYEISLERCEARGNVKISTIWKRFEWDEILNLEAPNDEDLNLSNWLKNRVGHELHVNTKYTYEDKIDFQVISLKNKDQKFDIIDIDWSK" }, "paralog": false, "identity": 0.9828080229226361, "score": 654.0, "evalue": 1.8e-240, "description": "Internal stop codon at 131714, 131735, 132350, 132365, 132422, 132548. Nonsense mutation around 131714, 131735, 132350, 132365, 132422, 132548.", "psc": { "uniref90_id": "UniRef90_D1J7P8", "gene": "MpfA", "product": "Hemolysin C", "uniref50_id": "UniRef50_A0A077L6Q7", "cog_id": "COG1253", "cog_category": "PT", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P8", "UniRef:UniRef50_A0A077L6Q7", "COG:COG1253", "COG:PT" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L6Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L6Q7" ], "product": "Hemolysin C" } }, "id": "AHCKFKLKGB_274", "locus": "GCA013367975_00239" }, { "type": "cds", "sequence": "contig_1", "start": 132734, "stop": 133174, "strand": "+", "frame": 2, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "KEGG:K02990", "RefSeq:WP_012855389.1", "SO:0001217", "UniParc:UPI0001BC20BC", "UniRef:UniRef100_D1J7P9", "UniRef:UniRef50_B3PN60", "UniRef:UniRef90_D1J7P9" ], "nt": "ATGTCAAATTACGAAATTATGATTCTAACTAATCCAAAAACAACAGATGAAGAATTACAAAATCTAGTATTTTCAGTATTAGATAAAAATTCATCAAAATTTGAAAGATTAGAAAGAAACGAAACTGCATATCCAATTAAAAAGTTAACTCATGTAAATTACTTTTTAATTACAACAAAGGCAGAACCTCATTTAATGTCTGAATTAACAAGAAAGCTTAATATCGATAAATCAGTTTTAAGATCTTTAATTATTAATTTAGATTCTGAAAAAGGTTTAAAGCCAAGAAAAGTTTCTAAGAAATTCAATAAGAGAATTCCTCTAAACCGTGAAAATAAAAAACCTTATGTAAAAAACAACGAAACTTCTAAAGATGCTAGCGAAGAAAAGAAGGTTGCAAGAAAACCAAGAACTTCAAAAGTTTTAGACGAAAAGAAATAG", "aa": "MSNYEIMILTNPKTTDEELQNLVFSVLDKNSSKFERLERNETAYPIKKLTHVNYFLITTKAEPHLMSELTRKLNIDKSVLRSLIINLDSEKGLKPRKVSKKFNKRIPLNRENKKPYVKNNETSKDASEEKKVARKPRTSKVLDEKK", "aa_hexdigest": "1f6220519f0b35fdeb0c010b1e0bdf54", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20BC", "ncbi_nrp_id": "WP_012855389.1", "uniref100_id": "UniRef100_D1J7P9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20BC", "RefSeq:WP_012855389.1", "UniRef:UniRef100_D1J7P9" ] }, "ips": { "uniref100_id": "UniRef100_D1J7P9", "uniref90_id": "UniRef90_D1J7P9" }, "psc": { "uniref90_id": "UniRef90_D1J7P9", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_B3PN60", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN60" ], "product": "Small ribosomal subunit protein bS6" }, "genes": [ "rpsF" ], "id": "AHCKFKLKGB_275", "locus": "GCA013367975_00240" }, { "type": "cds", "sequence": "contig_1", "start": 133203, "stop": 133361, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00436.31" ], "nt": "ATGAATAAAGTATTTTTAATTGGTCGTTTGTCGGCCAAACCTTATAAAAATGTTACACCTTCTAATGTTGAATATTCAAGATTTACTATGGCTATTAGACGCGAATACGCCGCCCCTAATTCTGAACCTGTAGTAGACTTTGTCCCATGTGTTGCTTGA", "aa": "MNKVFLIGRLSAKPYKNVTPSNVEYSRFTMAIRREYAAPNSEPVVDFVPCVA", "aa_hexdigest": "e3643d89a9a5ba85e9b34a3c195bc11e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00436.31", "name": "SSB", "length": 54, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.925947287775446e-09, "score": 32.86436462402344, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5877.7719000000025, "isoelectric_point": 9.426970100402833 }, "id": "AHCKFKLKGB_276", "locus": "GCA013367975_00241" }, { "type": "cds", "sequence": "contig_1", "start": 133521, "stop": 133661, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAAAATAGACGAAAAAATAACAATGGTAAAGAATTTAATATTGCCACAGAAGAATCAAACACTATCGATAATAATTTAGAAAATAATCATCAATCAAATGAATCAAATGATAATCCTTTTGATCAACTTGATTGA", "aa": "MSENRRKNNNGKEFNIATEESNTIDNNLENNHQSNESNDNPFDQLD", "aa_hexdigest": "27e71f40877bf2643a03d5e9efb89bb6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5352.431800000002, "isoelectric_point": 4.394188499450684 }, "id": "AHCKFKLKGB_277", "locus": "GCA013367975_00242" }, { "type": "cds", "sequence": "contig_1", "start": 133679, "stop": 133987, "strand": "+", "frame": 2, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "RefSeq:WP_044329438.1", "SO:0001217", "UniParc:UPI0005C9432E", "UniRef:UniRef100_UPI0005C9432E", "UniRef:UniRef50_D1J7Q1", "UniRef:UniRef90_D1J7Q1" ], "nt": "ATGGCAAGAATTGTTCGTAAGAAACCTTTTATTCGTAAACGTCCATGTCAATTTTGTTTAAGTAAAGAACCAGTTGTTTACATTGACTACAAAAATGAAGAAGTTTTATCAAAATTAGTAAATTTACAAGGAAAAATTTTATCATCACGTATTACCGGAACATGTGCAAAACACCAAAGAGCAGTTGCATTAGCAATTAAAAGAGCAAGATTTGTTGGTATTTTGCCATACATCGGTTTAGTAAAAAGAGAATCTAAAAAAGAAACTGTTAAAACTGAAAAAGTAGAAAAAACAGAAAAAGCAGAATAA", "aa": "MARIVRKKPFIRKRPCQFCLSKEPVVYIDYKNEEVLSKLVNLQGKILSSRITGTCAKHQRAVALAIKRARFVGILPYIGLVKRESKKETVKTEKVEKTEKAE", "aa_hexdigest": "3694139d864a63ad27348bd0acc2eced", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0005C9432E", "ncbi_nrp_id": "WP_044329438.1", "uniref100_id": "UniRef100_UPI0005C9432E", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C9432E", "RefSeq:WP_044329438.1", "UniRef:UniRef100_UPI0005C9432E" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C9432E", "uniref90_id": "UniRef90_D1J7Q1" }, "psc": { "uniref90_id": "UniRef90_D1J7Q1", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_D1J7Q1", "cog_id": "COG0238", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Q1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Q1" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "AHCKFKLKGB_278", "locus": "GCA013367975_00243" }, { "type": "cds", "sequence": "contig_1", "start": 134467, "stop": 134766, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_277872654.1", "SO:0001217", "UniParc:UPI0024119A9B", "UniRef:UniRef100_UPI0024119A9B", "UniRef:UniRef50_UPI001E51F6A9", "UniRef:UniRef90_UPI001E51F6A9" ], "nt": "GTGGCTAATATTTCCTTTAAATTTACATATGGAAAAAATTTTCAAAATCAAATTTCGGTTTATGAAACTAATAAATTTAATATGATCAATAGAGTATTAACGGCTTTTTTAGTATTATACATTTTATTTACTATTGCTTCTATTTCACTGAATATTTATGTTTCAATAATTTTATTCAAAAACAAGCATGCTAAACTCTTTTTACTTTCATTGGCAAGCGTTTTAGTTTCAAGAATAATTGGTGTTTTTTGCGGAATCATTCTGCTTTTAAATAAAAGACTTTCAACAAGCAAATGCTAA", "aa": "MANISFKFTYGKNFQNQISVYETNKFNMINRVLTAFLVLYILFTIASISLNIYVSIILFKNKHAKLFLLSLASVLVSRIIGVFCGIILLLNKRLSTSKC", "aa_hexdigest": "a0f64d5a9fcd9de150ba39a77ce3ddc6", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0024119A9B", "ncbi_nrp_id": "WP_277872654.1", "uniref100_id": "UniRef100_UPI0024119A9B", "db_xrefs": [ "SO:0001217", "UniParc:UPI0024119A9B", "RefSeq:WP_277872654.1", "UniRef:UniRef100_UPI0024119A9B" ] }, "ips": { "uniref100_id": "UniRef100_UPI0024119A9B", "uniref90_id": "UniRef90_UPI001E51F6A9" }, "psc": { "uniref90_id": "UniRef90_UPI001E51F6A9", "uniref50_id": "UniRef50_UPI001E51F6A9" }, "pscc": { "uniref50_id": "UniRef50_UPI001E51F6A9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E51F6A9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11272.486399999983, "isoelectric_point": 10.159202003479002 }, "id": "AHCKFKLKGB_279", "locus": "GCA013367975_00244" }, { "type": "cds", "sequence": "contig_1", "start": 134822, "stop": 135550, "strand": "+", "frame": 2, "gene": "rarA", "product": "ATPase, AAA family", "db_xrefs": [ "COG:COG2256", "COG:L", "KEGG:K07478", "SO:0001217", "UniRef:UniRef50_A0A077L8I4", "UniRef:UniRef90_D1J7Q3" ], "nt": "ATGGATTCTAACTTAGCAAATTTAATAAGGCCGAAAATTTTAGAAGATTTTATTTGCCCACAAGAACAAAAAAAATTATTTCAAAAAATTATAGAAAATAATGATTATCGTTCATTTATTTTTTATGGAAAGCCAGGTACCGGAAAAACAACTATTTCCTATATTCTTGCCAATGCCTTAAAAGTAAATTACGAATATTTTAATGCGGCTATTGAAAATAAAGATGAGCTTGTGCATAAATTAAAGTTGAACAAGATTTTGATCATTGATGAGATTCATAGATTAAACAAAGATAAACAAGATATATTGTTACCATATTTAGAAAACGATTTAATCACAATTTATGCAACTACAACGGAAAATCCATATTTTAAGCTTAATCCTGCATTACGTTCAAGATGTTCAATAATTGAAATTACTAAGCCAACAATTCAAGATTTATCAAGTTGTTTAAAAAATGCCTGCCTTAAAACTTTTGGTGTGGATCTTAATAAGGATATTGCTGATTATTTAGCCCTACAGGCTAATGGCGACTTTAGATCAGCATTAAACAATCTTGATTTGATTATGTTAATTTTTAAAGATCAAGAATTTTCATTGGAGGCAATTAAAAAGATTATTCCCTCAATTCAATTTAGTTCAGATGAATCAGGTGATAATCATTATGATTATTTAAGTGCTTTTCATAAATCACTACGCGGTTCGGACATTGATGCAGCATTATATTGA", "aa": "MDSNLANLIRPKILEDFICPQEQKKLFQKIIENNDYRSFIFYGKPGTGKTTISYILANALKVNYEYFNAAIENKDELVHKLKLNKILIIDEIHRLNKDKQDILLPYLENDLITIYATTTENPYFKLNPALRSRCSIIEITKPTIQDLSSCLKNACLKTFGVDLNKDIADYLALQANGDFRSALNNLDLIMLIFKDQEFSLEAIKKIIPSIQFSSDESGDNHYDYLSAFHKSLRGSDIDAALY", "aa_hexdigest": "dabf03921ebcdfee86d7496cc1c501dc", "start_type": "ATG", "rbs_motif": null, "genes": [ "rarA" ], "truncated": "3-prime", "pseudogene": { "start": 134822, "stop": 135853, "observations": { "mutation": [ 135548 ], "stop": [ 135548 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q3", "query_cov": 1.0, "subject_cov": 0.599009900990099, "identity": 0.992, "score": 479.0, "evalue": 1.66e-168, "gene_start": 1, "gene_end": 242, "reference_start": 1, "reference_end": 242, "reference_sequence": "MDSNLANLIRPKILEDFICPQEQKKLFQKIIENNDYRSFIFYGKPGTGKTTISYILANALKVNYEYFNAAIENKEDLVHKLKLNKILIIDEIHRLNKDKQDILLPYLENDLITIYATTTENPYFKLNPALRSRCSIIEITKPTIQDLSSCLKNACLKTFGVDLNKDIADYLALQANGDFRSALNNLDLIMLIFKDQEFSLEAIKKIIPSIQFSSDESGDNHYDYLSAFHKSLRGSDIDAALYWGFIIIKSGDFDGLFRRMLCASYEDVGMANPKLGSEVLSAIECFERIGMPEGYLPITQAILNICLSPKSNTVYSSSTLIKSIIDSGNIFEVPIHLKDAHYKSAAKLKRGLNYKYPHDYKNNYIDQQYLPNELKDKIHIELNGQGYEQKIKQYWDLIKNKGEK" }, "paralog": false, "identity": 0.9912536443148688, "score": 677.0, "evalue": 5.01e-249, "description": "Internal stop codon at 135548. Nonsense mutation around 135548.", "psc": { "uniref90_id": "UniRef90_D1J7Q3", "gene": "RarA", "product": "ATPase, AAA family", "uniref50_id": "UniRef50_A0A077L8I4", "cog_id": "COG2256", "cog_category": "L", "kegg_orthology_id": "K07478", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q3", "UniRef:UniRef50_A0A077L8I4", "COG:COG2256", "COG:L", "KEGG:K07478" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L8I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L8I4" ], "product": "ATPase" } }, "id": "AHCKFKLKGB_280", "locus": "GCA013367975_00245" }, { "type": "cds", "sequence": "contig_1", "start": 135587, "stop": 136006, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12002.13" ], "nt": "TTGTTTAGAAGAATGTTGTGTGCAAGTTACGAAGATGTTGGAATGGCAAATCCTAAATTGGGATCAGAGGTTTTGTCAGCAATTGAATGTTTTGAAAGAATAGGAATGCCTGAGGGGTATTTACCAATAACACAAGCAATATTAAATATTTGTTTAAGTCCAAAATCAAACACTGTTTATAGCTCATCAACATTAATAAAATCTATAATTGACAGTGGAAATATTTTTGAAGTGCCAATTCATTTAAAAGATGCTCATTATAAATCGGCAGCAAAACTAAAAAGAGGATTAAATTATAAATATCCTCATGATTATAAAAATAATTATATTGATCAACAATATCTACCAAATGAATTAAAAGATAAAATTCATATTGAATTAAATGGGCAAGGTTATGAACAAAAAATTAAACAATACTGA", "aa": "MFRRMLCASYEDVGMANPKLGSEVLSAIECFERIGMPEGYLPITQAILNICLSPKSNTVYSSSTLIKSIIDSGNIFEVPIHLKDAHYKSAAKLKRGLNYKYPHDYKNNYIDQQYLPNELKDKIHIELNGQGYEQKIKQY", "aa_hexdigest": "3c6fda4fe81798fb9208ff8e42ca8689", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12002.13", "name": "MgsA_C", "length": 137, "aa_cov": 0.9856115107913669, "hmm_cov": 1.0, "evalue": 3.389968602471293e-45, "score": 149.2564239501953, "start": 2, "stop": 138 } ], "seq_stats": { "molecular_weight": 15923.146799999979, "isoelectric_point": 8.34403018951416 }, "id": "AHCKFKLKGB_281", "locus": "GCA013367975_00246" }, { "type": "cds", "sequence": "contig_1", "start": 136038, "stop": 136355, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02912.23" ], "nt": "ATGGAATTTGATTTAAATAAAATAAATACATTGGAAGATTTAAAGTTAGCTAAAAATGCTTTCAATAATTCAGATGAGTTAAAAGAATTAATGTCTCAAATTAAAACAGCAGATCCATTGCAAAAAGCAGAACTTGGTAAAAAAATTCAACTGCTAAAAAAGCAAGCAGAAGATTTTTTTGATTTAGCAAAGCAAAAACTTGAAGATATAAAAATTCAAGATTTAATTAATCAAGAGTATGTAGATTTTGCAAATCCTATAAATACAACTGGAGGAATTCATCCAATTAATTTAGTTGCAAATAGATTTAGAGAATGA", "aa": "MEFDLNKINTLEDLKLAKNAFNNSDELKELMSQIKTADPLQKAELGKKIQLLKKQAEDFFDLAKQKLEDIKIQDLINQEYVDFANPINTTGGIHPINLVANRFRE", "aa_hexdigest": "399641ab99a851063a0cfdba480224fb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02912.23", "name": "Phe_tRNA-synt_N", "length": 64, "aa_cov": 0.6, "hmm_cov": 1.0, "evalue": 3.824761264881063e-08, "score": 28.557003021240234, "start": 11, "stop": 73 } ], "seq_stats": { "molecular_weight": 12088.696599999985, "isoelectric_point": 5.212385749816893 }, "id": "AHCKFKLKGB_282", "locus": "GCA013367975_00247" }, { "type": "cds", "sequence": "contig_1", "start": 136359, "stop": 136796, "strand": "+", "frame": 3, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "COG:COG0016", "COG:J", "EC:6.1.1.20", "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_C5J6V7", "UniRef:UniRef90_D1J7Q4" ], "nt": "TTGGCAAATGGATATTTCGAATATACGGGACCCGAAATAGAAAATGAAGAATATAATTTTGAACGTTTGAATATTCCCCAATCTCACCCTGCAAGAGAGATGCAAGATTCACTATATATATCAGAAAAAGAATTGCTTAGAACTCACAATACAGGATTTAGTGCAAGAGCATTAGAGGAACATAAAAATCAAGAGTTTTCACAATTCGCTATTGGGAAAGTTTACAGAAATGATGAAGAAGATAGAACACATACTCACCAATTTACTCAATTAGATTTTGTTAGTGTTGGTAACCATTCATTTGGAACATTAATTTATACATTGACTGAATTATTTTCTTATGTTTTAGAACAAAAGGTAAAAATTAGACTACGTCCTTCTTATTTTCCATTTACTGAACCTAGTGTTGAAGTTGATGTTTTGTTTAATGGAAAATGA", "aa": "MANGYFEYTGPEIENEEYNFERLNIPQSHPAREMQDSLYISEKELLRTHNTGFSARALEEHKNQEFSQFAIGKVYRNDEEDRTHTHQFTQLDFVSVGNHSFGTLIYTLTELFSYVLEQKVKIRLRPSYFPFTEPSVEVDVLFNGK", "aa_hexdigest": "1a51f5768bece1e6eef1d1a4be9c0bd3", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "pheS" ], "truncated": "both", "pseudogene": { "start": 136059, "stop": 136979, "observations": { "mutation": [ 136353, 136794 ], "stop": [ 136353, 136794 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q4", "query_cov": 1.0, "subject_cov": 0.46325878594249204, "identity": 0.986, "score": 288.0, "evalue": 5.36e-96, "gene_start": 1, "gene_end": 145, "reference_start": 108, "reference_end": 252, "reference_sequence": "MEFDLNKINTLEDLKLAKNAFNNSDELKELMSQIKTADPLQKAELGKKIQLLKKQAEDFFDLAKQKLEDIKIQDLINQEYVDFANPINTTGGIHPINLVANRFREWLLANGYFEYTGPEIENEEYNFERLNIPQSHPAREMQDSLYISEKELLRTHNTGFSARALEEHKNQEFSQFAIGKVYRNDEEDRTHTHQFTQLDFVSVGNHSFGTLIYTLTELFSYVLEQKVKIRLRPSYFPFTEPSVEVDVLFNEKWVEVLGAGMLHENVLKMAGYTNDMNGIAAGIGIERITMIKYGIDDIRELYGNDQRFLKQFK" }, "paralog": false, "identity": 0.9901960784313726, "score": 601.0, "evalue": 3.75e-222, "description": "Internal stop codon at 136353, 136794. Nonsense mutation around 136353, 136794.", "psc": { "uniref90_id": "UniRef90_D1J7Q4", "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_C5J6V7", "cog_id": "COG0016", "cog_category": "J", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q4", "EC:6.1.1.20", "UniRef:UniRef50_C5J6V7", "COG:COG0016", "COG:J", "KEGG:K01889", "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_C5J6V7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C5J6V7" ], "product": "Phenylalanine--tRNA ligase alpha subunit" } }, "id": "AHCKFKLKGB_283", "locus": "GCA013367975_00248" }, { "type": "cds", "sequence": "contig_1", "start": 136818, "stop": 136979, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGTTGCATGAAAACGTTTTAAAAATGGCCGGATATACCAATGATATGAATGGCATTGCTGCAGGAATTGGTATTGAAAGAATAACAATGATAAAGTATGGCATTGACGATATAAGAGAATTGTATGGAAATGATCAAAGATTTTTAAAACAATTTAAATAA", "aa": "MLHENVLKMAGYTNDMNGIAAGIGIERITMIKYGIDDIRELYGNDQRFLKQFK", "aa_hexdigest": "247ce24247d8c02fcba2a60b9e877202", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 52, "aa_cov": 0.9811320754716981, "hmm_cov": 1.0, "evalue": 8.745560420312303e-20, "score": 66.50789642333984, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 6107.0264, "isoelectric_point": 6.533664894104004 }, "id": "AHCKFKLKGB_284", "locus": "GCA013367975_00249" }, { "type": "cds", "sequence": "contig_1", "start": 136991, "stop": 137704, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGTTATTTTCTTATAAAAAAATAGTTAAATTGGCAAATATAACTGACAAAAGTGTAGAAGAGGTTGTTGGGGCAATTAATTCAATAGGCTTTGAAGTTGAAGAATATCATAAGTTTCTAGATGCCGAAGGTATGAAATTCTGTCATGTATTAAAGGCATACAAAAATCCTAATTCCGATCGTTTAACAGTTTGTGAGGTTGAATTTGGTAATGGAAATCATTCAATAATTCAAACCAATGCAACAAACATGCATGAAGGCGATTATGTAATGGCTTATGTTCCTGGATCAAGATCTGGAAAAAATGTATTTGCTGCCAAGGAACTAAAAGGAATCATCTCTGAAGGAATGTTTGTAAGTTTGAATGAAATTGGTATTCCAGAAGAATTTCATCCTTTAGATTCAAAAGATGGTATTTTTCAAGTTGGAAAAATAGATTTAAATTTAGACCCTGTTCAATATTTTGATCTTGATGATTATTTGATTGATGTAACGATTTTATCTAATAGAGCTGATGCATTGTGCTATTTAATTTTAGCTAAAGAAATTTCAGCATATTTTGAATCATATGTTGAACCTCTTTCAAAACCAAAACCTACTTTGGAAAGCGATATTGTTGTAAGTAAAAATTTAGTTGCTACAAACTGTTTTAGTTTAGTTGAAGCAACTACTAAACAACCTACTATTTCTCTTCAAGATGAATTTCTTTTATGA", "aa": "MLFSYKKIVKLANITDKSVEEVVGAINSIGFEVEEYHKFLDAEGMKFCHVLKAYKNPNSDRLTVCEVEFGNGNHSIIQTNATNMHEGDYVMAYVPGSRSGKNVFAAKELKGIISEGMFVSLNEIGIPEEFHPLDSKDGIFQVGKIDLNLDPVQYFDLDDYLIDVTILSNRADALCYLILAKEISAYFESYVEPLSKPKPTLESDIVVSKNLVATNCFSLVEATTKQPTISLQDEFLL", "aa_hexdigest": "cc22ce2e74b2c05dbaadbcc4aa630543", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI001F47B76D", "query_cov": 1.0126582278481013, "subject_cov": 1.00418410041841, "identity": 0.5, "score": 220.0, "evalue": 8.85e-69, "valid": false, "uniref50_id": "UniRef50_A0AAV4HE63" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 82, "aa_cov": 0.3291139240506329, "hmm_cov": 0.9146341463414634, "evalue": 1.4744835936378977e-09, "score": 33.22843551635742, "start": 46, "stop": 123 } ], "seq_stats": { "molecular_weight": 26450.893000000025, "isoelectric_point": 4.7467039108276365 }, "id": "AHCKFKLKGB_285", "locus": "GCA013367975_00250" }, { "type": "cds", "sequence": "contig_1", "start": 137750, "stop": 139129, "strand": "+", "frame": 2, "gene": "eMAP", "product": "phenylalanine--tRNA ligase subunit beta", "db_xrefs": [ "COG:COG0073", "COG:J", "EC:6.1.1.20", "KEGG:K01890", "SO:0001217", "UniRef:UniRef50_A0A448ZZ89", "UniRef:UniRef90_D1J7Q5" ], "nt": "TTGGTCTTATTATATGCGGGAGTTCCTTGTCATGTATATAATAAAAATTCTTTAAGTTCAAATGAGTTTTCTACATCATTGAGCAATGAAACATTAAATGTCTTAGGCAATAAACAAGTTACATTAAACAACAATTTGGTTGTAAAAAATGGAGATCAAACTGTTTCAATAGCAAGTGTTATAGGACTAGAAAATTATGAATATAGACCCGATGGTGGACATTGCGTCTTTGAATTAGCATCATTTTCGTTAAAAGAAGTTAGAAGAAGTATAAAAACAGTCAAATTAGAAACATTGGCATCTTTACGGGGCTCAAAAGAAATATCATGTGGTCAAGTTGTTATGGCATATGAATTTTTAACTAAATATTTAGATCAATATTCAGTTCAAATAAACGCACCAAAGCAAAGAAAAACAAATATATTAATTGATAGTCATTATATTACTAAATATGCGGGCTTTAATATTACAAAAACCAAAAAATATATTCAAGTCTTAAATCAATTAACTCTTTTGGGCTTTAAAATAAAGGAAGATTTATCATCAGTAATTTTTCCAACTTATAGATACGATTTAAAAAATATGCAAGATTTTGTTGAAGAAGTGTTTAGATTTTATGGATATGATAATTTCCCAGCTAAATCTCCAAAAATTACTAGATTATTAATTAAAGAAAACAAAGAATTTAAATTTATTGAAAACTTCTGTGCAAAAGGATATTTCAATGTTAGAACATACACGTTAATTAATCCTGAAAAAAACATTTTTAATCCATTTGATTTTAAAACTAATTTCAATGCTATTGCAGCAAAGAATTATGATCATTCTCAAATTAGAAATTCAATGATTTCTTCATTATTAGAAGTCTTGGATACAAATATCAAACAAGGTTTGCCAGCTGCTTCTTATTTTGAAATTGCTATGATTAATAATAAAATGAATGTATTAGGAATTGCTTCATTAAATAAAACTATTGATGATATAAAAGCTGATATTTGTTCAATAACTAATGAAACATTAGAATTTAAACCAAGTAAATTAAAAATTTTTAATCCAAATGCAGCACAAGAAATTTATTTAAATAACGAATTAATTGGATATGTTGCAAAATTAGACCCTCACTATTGTCAATATAATGCAGTGTTTAGCGAAGTCTTTTTAGATAAAATTAATAGCAACGTAATTAAATATAAAAATTATAAGCACACACCTCTTAAATCTAGGGATGTTACTATATCTGTTTTAGATAAACAATCTATTGAAGAAACAATTAAAAAAATACAAAGTATTTTTGGCGTGTACAAGATTTCAATTAAGGATGTTTATAATAAAGATAATGGCACAAAAAATATTACATTAGAAGTAATTTTAGAAGATTGA", "aa": "MVLLYAGVPCHVYNKNSLSSNEFSTSLSNETLNVLGNKQVTLNNNLVVKNGDQTVSIASVIGLENYEYRPDGGHCVFELASFSLKEVRRSIKTVKLETLASLRGSKEISCGQVVMAYEFLTKYLDQYSVQINAPKQRKTNILIDSHYITKYAGFNITKTKKYIQVLNQLTLLGFKIKEDLSSVIFPTYRYDLKNMQDFVEEVFRFYGYDNFPAKSPKITRLLIKENKEFKFIENFCAKGYFNVRTYTLINPEKNIFNPFDFKTNFNAIAAKNYDHSQIRNSMISSLLEVLDTNIKQGLPAASYFEIAMINNKMNVLGIASLNKTIDDIKADICSITNETLEFKPSKLKIFNPNAAQEIYLNNELIGYVAKLDPHYCQYNAVFSEVFLDKINSNVIKYKNYKHTPLKSRDVTISVLDKQSIEETIKKIQSIFGVYKISIKDVYNKDNGTKNITLEVILED", "aa_hexdigest": "541fb30e1d91ab77dfb569186050fe66", "start_type": "TTG", "rbs_motif": null, "genes": [ "eMAP" ], "truncated": "both", "pseudogene": { "start": 137450, "stop": 139165, "observations": { "mutation": [ 137702, 139127 ], "stop": [ 137702, 139127 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q5", "query_cov": 1.0, "subject_cov": 0.6339779005524862, "identity": 0.987, "score": 890.0, "evalue": 0.0, "gene_start": 1, "gene_end": 459, "reference_start": 254, "reference_end": 712, "reference_sequence": "MLFSYKKIVKLANITDKSVEEVVGAINSIGFEVEEYHKFLDAEGMKFCHVLKAYKNPNSDRLTVCEVEFGNGNHSIIQTNATNMHEGDYVMAYVPGSRSGKNIFAAKELKGIISEGMFVSLNEIGIPEEFHPLDSKDGIFQVGKIDLNLDPVEYFDLDDYLIDVTILSNRADALCYLILAKEISAYFESYIEPLSKPKPTLESNIVVSKNLVATNCFSLVEAITKQPAISLQDEFLLWKHGIKTFRNAVDITNLVLLYAGVPCHVYNKNSLSSNEFSTSLSNETLNVLGNKQVTLNNNLVVKNGDQTVSIASVIGLENYEYRPDGGHCVFELASFSLKEVRKSIKTVKLETLASLRGSKEISSGQVVMAYEFLTKYLDQYSVQINAPKLRKTNILIDSHYITKYAGFNITKTKKYIQVLNQLTLLGFKIKEDLSSVIFPTYRYDLKNMQDFVEEVFRFYGYDNFPAKSPKITRLLIKENKEFKFIENFCAKGYFNVRTYTLINPEKNIFNPFDFKTNFNAIAAKNYDHSQIRNSMISSLLEVLDTNIKQGLPDASYFEIAMINNKMNVLGIASLNKTIDDIKADICSITNETLEFKPSKLKIFNPNAAQEIYLNNELIGYVAKLDPHYCQYNAVFSEVFLDKMNSNVIKYKNYKHTPLKSRDVTISVLDKQSIEETIKKIQSIFGVYKISIKDVYNKDNGTKNITLEVILEDWATKKFDSIFNK" }, "paralog": false, "identity": 0.9807355516637478, "score": 1103.0, "evalue": 0.0, "description": "Internal stop codon at 137702, 139127. Nonsense mutation around 137702, 139127.", "psc": { "uniref90_id": "UniRef90_D1J7Q5", "gene": "EMAP", "product": "phenylalanine--tRNA ligase subunit beta", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_A0A448ZZ89", "cog_id": "COG0073", "cog_category": "J", "kegg_orthology_id": "K01890", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q5", "EC:6.1.1.20", "UniRef:UniRef50_A0A448ZZ89", "COG:COG0073", "COG:J", "KEGG:K01890" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZ89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZ89" ], "product": "Phenylalanine--tRNA ligase beta subunit" } }, "id": "AHCKFKLKGB_286", "locus": "GCA013367975_00251" }, { "type": "cds", "sequence": "contig_1", "start": 139263, "stop": 139448, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAACAACAAATGATAATCTTCAAAAAGAAAAGAGCATTATTGCTGAAACATTAAAGGAAATAGAAAAGAAATTTGGCAAAGAAGCCGTTATGATTTTAGGAGAAAAGCCAAGCATAATTCCCGAAACTTTTAGTAGTGGTTCGTTGACTATTGATAGAGCGTTAGGAATAAATGGATGA", "aa": "MKETTNDNLQKEKSIIAETLKEIEKKFGKEAVMILGEKPSIIPETFSSGSLTIDRALGING", "aa_hexdigest": "c9f41f4d8b6240cde5449c81972c0dfd", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6693.649199999999, "isoelectric_point": 5.342262840270995 }, "id": "AHCKFKLKGB_287", "locus": "GCA013367975_00252" }, { "type": "cds", "sequence": "contig_1", "start": 139509, "stop": 140150, "strand": "+", "frame": 3, "gene": "recA", "product": "Protein RecA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9RV21" ], "nt": "TTGCACGCTATTGCCGAAATACAAAAAACTGGTGGAATTGCAGCATTTATTGATGCTGAGCATTCTATAGATCCAATATATGCCAAAAATTTAGGGGTTGATATTGACAATTTAATTTTATCTCAACCAGATTCAGGAGAACAAGCATTAGAAATTGTTGATACATTGTCTAAATCTGGGGCAATAGGACTAATTGTTGTCGATTCGGTCGCTGCATTGGTTCCTGAGGCTGAGTTGAATGGAGAAATGAAAGACCAAGTAGTAGGTGCACAGGCAAGGCTTATGTCTAAAGCCTTAAGAAAAATAACTGGATCATTATCAAAAAACAAAACAACAGTAATATTTATTAATCAAATAAGAGAAAAAATTGGAGTTATTTTTGGCAATCCTGAAACAACAACTGGCGGAAGAGCATTGAAATTTTATTCAAGCATCCGTCTTGAAGTTAGAAAAATTCAAAATGTAACCAATAACGGAGACATTACAGGTAATCAAATTAAGTGTAAAGTTGTAAAGAACAAATTAGCTGCTCCGTATAAAACTGCTCAAATTGATATTATTTTTTCAAAAGGTATTTCAAAAGAGCTTGAAATAATTAATTTAGCAGAAGAATTAGGAATTTTAGTAAAAAAGGGCTCGTGA", "aa": "MHAIAEIQKTGGIAAFIDAEHSIDPIYAKNLGVDIDNLILSQPDSGEQALEIVDTLSKSGAIGLIVVDSVAALVPEAELNGEMKDQVVGAQARLMSKALRKITGSLSKNKTTVIFINQIREKIGVIFGNPETTTGGRALKFYSSIRLEVRKIQNVTNNGDITGNQIKCKVVKNKLAAPYKTAQIDIIFSKGISKELEIINLAEELGILVKKGS", "aa_hexdigest": "b0f1378336773daef7e2d17b28aa8210", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A0D5BUH9", "query_cov": 0.9765258215962441, "subject_cov": 0.9244444444444444, "identity": 0.625, "score": 266.0, "evalue": 9.97e-88, "valid": false, "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_A0A7U9RV21", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9RV21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9RV21" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "AHCKFKLKGB_288", "locus": "GCA013367975_00253" }, { "type": "cds", "sequence": "contig_1", "start": 140316, "stop": 140540, "strand": "+", "frame": 3, "gene": null, "product": "Metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "nt": "ATGAAAAATGAAAATATAAAAATTCTATTTTTGGGAGACATATTTGGTTTACCAGGAATTAGATTTGTTGAGCAACAATTGCCAAACATAATAAAACAAAATAATATTGATTTTGTAATCGCTCAAGCAGAAAACGTTTCAGGAAGAAAGGGCTTTGAGCCTAAGGATTATGAAAGATTAAAAAGAGCAGGAATAAATGCATTTACATTAGGAAATCACGTTTGA", "aa": "MKNENIKILFLGDIFGLPGIRFVEQQLPNIIKQNNIDFVIAQAENVSGRKGFEPKDYERLKRAGINAFTLGNHV", "aa_hexdigest": "d1de8a61c0e753d54d0f0f87de7e0890", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FBC5", "query_cov": 0.9324324324324325, "subject_cov": 1.0, "identity": 0.536, "score": 89.0, "evalue": 6.56e-22, "valid": false, "product": "Metallophosphoesterase", "uniref50_id": "UniRef50_A0A432FBC5" }, "pscc": { "uniref50_id": "UniRef50_A0A432FBC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "product": "Metallophosphoesterase" }, "genes": [], "id": "AHCKFKLKGB_289", "locus": "GCA013367975_00254" }, { "type": "cds", "sequence": "contig_1", "start": 140901, "stop": 141122, "strand": "+", "frame": 3, "gene": null, "product": "Putative metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PKR0" ], "nt": "TTGCCTAAGGGTACATATTTTGTAACTGATGCCGGAATGTGTGGTCCAAGAGATTGCGCTATTGGTTCTGACTTCCAAGAAGTATATGAAAAAATGCGTTACGCAGCATTTTCTGCATTTAAAGTATCAAAAAATAAATGTCAATTAAATGGAGTTATTTTTACATTAACTAAAGATCCAACAGCAAAGCATATTGAGTTAGTAAATATAAAGGAGCAATAA", "aa": "MPKGTYFVTDAGMCGPRDCAIGSDFQEVYEKMRYAAFSAFKVSKNKCQLNGVIFTLTKDPTAKHIELVNIKEQ", "aa_hexdigest": "442d0c506a340999cb8e039bf86e36d6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PKR0", "query_cov": 0.9863013698630136, "subject_cov": 0.9863013698630136, "identity": 0.667, "score": 107.0, "evalue": 2.36e-29, "valid": false, "product": "Putative metallophosphoesterase", "uniref50_id": "UniRef50_A0A3B0PKR0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PKR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PKR0" ], "product": "Putative metallophosphoesterase" }, "genes": [], "id": "AHCKFKLKGB_290", "locus": "GCA013367975_00255" }, { "type": "cds", "sequence": "contig_1", "start": 141124, "stop": 141384, "strand": "+", "frame": 1, "gene": "nusB", "product": "N utilization substance B-like protein(Protein nusB)", "db_xrefs": [ "COG:COG0781", "COG:K", "SO:0001217", "UniRef:UniRef50_D1J7Q8", "UniRef:UniRef90_D1J7Q8" ], "nt": "ATGAATAACGATTCATCATCAAAAATCAGTGAGAAGGATAAACGAATTTTATTTCTACTCAATAAATATAAGGCAAGGATGCATATTATTAATTGCATATATCAGAGCGAGCTATTTAATAAGAATCTTTCAATACAAGAAATTTTGGAAAATTGTGGAGAAAATCAATTGAATGCCAATGAAATAGAATCTTTAAAAATAATTGAAGCTAAATATGATGTTTTTACTAGAATAGCAAAAAAATTTATTAGTGAAGACTGA", "aa": "MNNDSSSKISEKDKRILFLLNKYKARMHIINCIYQSELFNKNLSIQEILENCGENQLNANEIESLKIIEAKYDVFTRIAKKFISED", "aa_hexdigest": "63cf68f1efa722f3473afd29320ff0de", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "nusB" ], "truncated": "3-prime", "pseudogene": { "start": 141124, "stop": 141597, "observations": { "mutation": [ 141382, 141388 ], "stop": [ 141382, 141388 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q8", "query_cov": 1.0, "subject_cov": 0.5477707006369427, "identity": 1.0, "score": 166.0, "evalue": 4.96e-51, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MNNDSSSKISEKDKRILFLLNKYKARMHIINCIYQSELFNKNLSIQEILENCGENQLNANEIESLKIIEAKYDVFTRIAKKFISEDWEWNRILPLSRAIIIYGEYELLFNDPRVVINEMVNISKIYSPNNDYKFINKVLDLVSKNVIKKNTKDEKEN" }, "paralog": false, "identity": 0.9872611464968153, "score": 303.0, "evalue": 1.42e-107, "description": "Internal stop codon at 141382, 141388. Nonsense mutation around 141382, 141388.", "psc": { "uniref90_id": "UniRef90_D1J7Q8", "gene": "NusB", "product": "N utilization substance protein B homolog(Protein nusB)", "uniref50_id": "UniRef50_D1J7Q8", "cog_id": "COG0781", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q8", "UniRef:UniRef50_D1J7Q8", "COG:COG0781", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Q8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Q8" ], "product": "N utilization substance protein B homolog(Protein nusB)" } }, "id": "AHCKFKLKGB_291", "locus": "GCA013367975_00256" }, { "type": "cds", "sequence": "contig_1", "start": 141478, "stop": 141597, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAATATTTCAAAAATATACAGTCCTAATAATGATTATAAATTTATCAATAAGGTTCTAGATCTTGTTTCTAAAAATGTAATTAAAAAAAATACTAAAGATGAAAAAGAAAATTAG", "aa": "MVNISKIYSPNNDYKFINKVLDLVSKNVIKKNTKDEKEN", "aa_hexdigest": "348a48353fc4b62036a2aff9bc7366ba", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4585.2389, "isoelectric_point": 9.399055290222169 }, "id": "AHCKFKLKGB_292", "locus": "GCA013367975_00257" }, { "type": "cds", "sequence": "contig_1", "start": 141581, "stop": 142315, "strand": "+", "frame": 2, "gene": "yqxC", "product": "Hemolysin A", "db_xrefs": [ "COG:COG1189", "COG:J", "EC:2.1.1.226", "EC:2.1.1.227", "KEGG:K06442", "RefSeq:WP_175933406.1", "SO:0001217", "UniParc:UPI00158F61C7", "UniRef:UniRef100_UPI00158F61C7", "UniRef:UniRef50_A0A077LAY8", "UniRef:UniRef90_D1J7Q9" ], "nt": "ATGAAAAAGAAAATTAGCGAGATAATAACCAATATTTTTAAATACGATTTGAAAATTTCAGAAGCGTTAATTAGAGAAGGAAGAGTAATTGTAAATAATGAAAAGATAATTATTCCATCATTAAAATTTGAAGAAACCGATGTAGATATAAGATTCATTAATATGAAATTGCCCTATGTTTCAAGAGGCGCTTATAAATTATTAGAAGCAATAGAAAAATTTAATATAGACTTAACGGATAAAATATGTCTTGACATAGGATCGTCTACTGGGGGATTTGTTGATGTAATGTTAAGGCATGGTGCTAAAAAAGTATATGCCGTAGATTGTGGCACAAATCAACTAGCATATGAACTTAGGATAAATGATAAGGTTGTTGTTTATGAAAAAACTAATCTTAAAAATTTAAAAAAGGAAATGTTCCTTGAAAATATTGAATTTGTATCTTGTGATGTTTCATTTATAAGCTTGAAATATGTATTTGAGGTTTGCAATAAAATATTAAATAATAATATAAATGTAGTTTTTTTAATTAAACCCCAATTTGAAGCTTCAAGCAAATATGTTGAACCCGGAGGATATGTTAATCAAGAAAATCATAAATATATTATTGATAAAGTAATTTCATATGCAAAAAATAATGGCTTTGTTTTGATTGATTTAATAAAAAGCCCTATTTTAGGAGAAAAATCAAAAAATATTGAATATTTAGGCTTTTTTAGAAAGGATAGTTAA", "aa": "MKKKISEIITNIFKYDLKISEALIREGRVIVNNEKIIIPSLKFEETDVDIRFINMKLPYVSRGAYKLLEAIEKFNIDLTDKICLDIGSSTGGFVDVMLRHGAKKVYAVDCGTNQLAYELRINDKVVVYEKTNLKNLKKEMFLENIEFVSCDVSFISLKYVFEVCNKILNNNINVVFLIKPQFEASSKYVEPGGYVNQENHKYIIDKVISYAKNNGFVLIDLIKSPILGEKSKNIEYLGFFRKDS", "aa_hexdigest": "ead2769a2db35db17b71557ce05e8572", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158F61C7", "ncbi_nrp_id": "WP_175933406.1", "uniref100_id": "UniRef100_UPI00158F61C7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158F61C7", "RefSeq:WP_175933406.1", "UniRef:UniRef100_UPI00158F61C7" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158F61C7", "uniref90_id": "UniRef90_D1J7Q9" }, "psc": { "uniref90_id": "UniRef90_D1J7Q9", "gene": "YqxC", "product": "Hemolysin A", "ec_ids": [ "2.1.1.226", "2.1.1.227" ], "uniref50_id": "UniRef50_A0A077LAY8", "cog_id": "COG1189", "cog_category": "J", "kegg_orthology_id": "K06442" }, "pscc": { "uniref50_id": "UniRef50_A0A077LAY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LAY8" ], "product": "Hemolysin A" }, "genes": [ "yqxC" ], "id": "AHCKFKLKGB_293", "locus": "GCA013367975_00258" }, { "type": "cds", "sequence": "contig_1", "start": 142315, "stop": 143007, "strand": "+", "frame": 1, "gene": "csdA", "product": "Nitrogen fixation protein NifS(Aminotransferase protein S)", "db_xrefs": [ "COG:COG0520", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZVE8", "UniRef:UniRef90_D1J7R0" ], "nt": "ATGAATTATAAGGATTTATTTCCTATGTTTAAAAATAACCCTGGTATTGTATATTTCGATAATTCAGCACTTACTTTTAAACCACAAACCGTAATAAATTCAGGTATTGATTTTTATGAAAAATATTCAATTTCTACAAGGACTGCTGATAGTGTTTTAGGAATACAAACAGAAGAAGTAATGCATGAAACTAGAAAAAAAATTGCAGATTTGATTGACGCTTCTGCCGATCAAACTATATTTACTTCAGGAACGACTGATGGATTAAACTTAATATCTTTAATGTTAAAAGATATAATAAAGGATGGCGAAATAATATTTAGCTATTTTAATCACAGCTCAGCAATCGTTCCATTCATTGAAAATCTAAAAAACAATAATATAACATTTAAATATGCAAAAGATAACGAGGAAGTTTTAGATTTAATAAATGAAAAAACGAAGTTAATTGTATTGCCTCAATTAACTAATAATTTTAATGTATTTTTTGATTTAAAAAAAATTCATCAAAAATCTAAAAAATATGGTGCATATTTGATAAACGATGCCGCACAAGCTATTGCTCACGAAAAAGTTTCATTGAACGACTGCGATGTAATTGTTTTTTCAAGCAATAAAATTTATGGTCCTACCGGTATGGGAGCTTTAGTAATTGAAAAAGATTTACTAAAAAAAATAGTTCCTCAAAAATGA", "aa": "MNYKDLFPMFKNNPGIVYFDNSALTFKPQTVINSGIDFYEKYSISTRTADSVLGIQTEEVMHETRKKIADLIDASADQTIFTSGTTDGLNLISLMLKDIIKDGEIIFSYFNHSSAIVPFIENLKNNNITFKYAKDNEEVLDLINEKTKLIVLPQLTNNFNVFFDLKKIHQKSKKYGAYLINDAAQAIAHEKVSLNDCDVIVFSSNKIYGPTGMGALVIEKDLLKKIVPQK", "aa_hexdigest": "41d7d379d2c52ff35cd02ac2e9d71ce2", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "csdA" ], "truncated": "3-prime", "pseudogene": { "start": 142315, "stop": 143310, "observations": { "mutation": [ 143005, 143047 ], "stop": [ 143005, 143047 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7R0", "query_cov": 1.0, "subject_cov": 0.5912596401028277, "identity": 0.983, "score": 446.0, "evalue": 7.31e-156, "gene_start": 1, "gene_end": 230, "reference_start": 1, "reference_end": 230, "reference_sequence": "MNYKDLFPMFKNNPGIVYFDNSALTFKPQTVINSGIDFYEKYSISTRTADSVLGIQTEEVIHETRKKIADLIDASANQTIFTSGTTDGLNLISLMLKDIIKDGEIIFSYFNHSSAIVPFIENLKNNNITFKYAKDNEEVLDLINEKTRLIVLPQLTNNFNVFFDLKKIHQKSKKYGAYLINDAAQAIAHEKVSLNDCDVIVFSSNKIYGPTGMGALVIEKDLLEKIVPQKWGGGQVQSIGSGCEWSAKNDIKKFEPGTPNFAGIFQFNAAIDFINSLGYELINKLETAVANYCYDELKTIDGIEIDSKRGDKIILFNIKNIPSQDIASYLGHNNIYVRSGVFCAHKFHELKQYSNSYVRISISFYNDYNDVDKFIKLLKEMGANFLEFL" }, "paralog": false, "identity": 0.9788519637462235, "score": 644.0, "evalue": 3.16e-236, "description": "Internal stop codon at 143005, 143047. Nonsense mutation around 143005, 143047.", "psc": { "uniref90_id": "UniRef90_D1J7R0", "gene": "CsdA", "product": "Nitrogen fixation protein NifS(Aminotransferase protein S)", "uniref50_id": "UniRef50_A0A448ZVE8", "cog_id": "COG0520", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7R0", "UniRef:UniRef50_A0A448ZVE8", "COG:COG0520", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVE8" ], "product": "Probable cysteine desulfurase" } }, "id": "AHCKFKLKGB_294", "locus": "GCA013367975_00259" }, { "type": "cds", "sequence": "contig_1", "start": 143471, "stop": 143920, "strand": "+", "frame": 2, "gene": "iscU", "product": "Aminotransferase protein U-like protein (Nitrogenfixation protein NifU)", "db_xrefs": [ "COG:COG0822", "COG:O", "RefSeq:WP_020002692.1", "SO:0001217", "UniParc:UPI0003650E82", "UniRef:UniRef100_A0A6A8PZC5", "UniRef:UniRef50_D1J7R1", "UniRef:UniRef90_D1J7R1" ], "nt": "TTGAATTTTTATAGTAATATTGAAAAACAAAGAATAATTTTTGAAGCATACGAAAACCCAAAATTTAAAATAAAAAATGATGAAGATTTTAAGAAAACTGATAAGACATTATTTGAACATTCAAATGTATGCGTTGACGATATTAAGCTAAATTTTTTATTTGATGAAAAAAATACTTTGAAAAAAGTGGAATATTGTGCTAAAGGGTGTTCAATTTTTTTGTCATCTGTTGAATTAATGATTCAGCAAATTTTGAATAAAAATAAATTAGAAATAAATGAAATTTTAAATGAATATAAAACAATGATAGATACATCTACAATTTCACAACGTGGTGGAGAAATATTGGGAAAATTGGTTGTATATCAAAATGTAAGAGTTCATTTAAATAGGTTAGAATGTGCATCAATAATTTTTAGGTTAATAAAAAAAGCAGTTTCTAATGAATAA", "aa": "MNFYSNIEKQRIIFEAYENPKFKIKNDEDFKKTDKTLFEHSNVCVDDIKLNFLFDEKNTLKKVEYCAKGCSIFLSSVELMIQQILNKNKLEINEILNEYKTMIDTSTISQRGGEILGKLVVYQNVRVHLNRLECASIIFRLIKKAVSNE", "aa_hexdigest": "8b4703889b764e3cc14019fde846bcfe", "start_type": "TTG", "rbs_motif": "GGxGG", "ups": { "uniparc_id": "UPI0003650E82", "ncbi_nrp_id": "WP_020002692.1", "uniref100_id": "UniRef100_A0A6A8PZC5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003650E82", "RefSeq:WP_020002692.1", "UniRef:UniRef100_A0A6A8PZC5" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PZC5", "uniref90_id": "UniRef90_D1J7R1" }, "psc": { "uniref90_id": "UniRef90_D1J7R1", "gene": "IscU", "product": "Aminotransferase protein U homolog (Nitrogenfixation protein NifU)", "uniref50_id": "UniRef50_D1J7R1", "cog_id": "COG0822", "cog_category": "O" }, "pscc": { "uniref50_id": "UniRef50_D1J7R1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7R1" ], "product": "Aminotransferase protein U homolog (Nitrogenfixation protein NifU)" }, "genes": [ "iscU" ], "id": "AHCKFKLKGB_295", "locus": "GCA013367975_00260" }, { "type": "cds", "sequence": "contig_1", "start": 143913, "stop": 144806, "strand": "+", "frame": 3, "gene": "dinP", "product": "DNA-damage repair protein MucB", "db_xrefs": [ "COG:COG0389", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J7R2", "UniRef:UniRef90_D1J7R2" ], "nt": "ATGAATAAGATAATTTTTCACATAGATATGGACTCCTTCTTTGTTAGTTGTGAACGTTCTATTAATACAAACTTAATAGACAAGCCTGTTGTAATTGCTAAACGATACAAACGTTCAATAATTAGTGCAATGTCTTACGAGGTAAAAAATCTTGGATATAGAGTAGGAAGTTCTTTATACACCGTTTTAGAAAAGGTTCCTAATTTGCTTATAGTAGAACCACACTATGATTTATATTCTCTAATTTCTAAAAAAATTTTTGATTATCTAAAAAATAAGTATTGTACACAACTAGAAATATATTCAATAGACGAATGCTATATTGATTTTAGCAACACTGTTAGCAATTGGGAGGAAGCTAAAAATCTTGCCCAACAAATTAAAGATGATATTTTAAAAACATTTATGATTCCTTGTTCAATAGGAATTTCCTTTACAAAGTTTTTGGCAAAAATGAGTACAAATAAAGCAAAACCATCAGGAATTTTAATAACAAAAAAGGAAGATATTGAAAGAAATTTTTACTCATTGCCGGTGGAAAAAATATTTGGGGTAGGAAAAAATATTTCACAACAATTAATAAAAAACAAAATTTCTACTTATAAAGATTTGTTAGAATGCAATAATGAAATTTTTTTAAGAAAATTGTTTAAAAAAAATTTTTTCTTATTTATTGAACAACTAAAGGGAATCAATGCAACCGACCACATCTTAAATCTTGAAACAAAAGGAATAAGTAACACGATAACTTTTATGGAAAAAGATTCTGATGATATCGTTTTTTTGAAAAATGAATTGTTTAAATTGGCAAGAAATGTTGCTAATAGGGCCGTAAACAACAATTATGAATGCAGATGTGTTGAAGTTTTAATAAGAAAACAAGACAAAAAATGA", "aa": "MNKIIFHIDMDSFFVSCERSINTNLIDKPVVIAKRYKRSIISAMSYEVKNLGYRVGSSLYTVLEKVPNLLIVEPHYDLYSLISKKIFDYLKNKYCTQLEIYSIDECYIDFSNTVSNWEEAKNLAQQIKDDILKTFMIPCSIGISFTKFLAKMSTNKAKPSGILITKKEDIERNFYSLPVEKIFGVGKNISQQLIKNKISTYKDLLECNNEIFLRKLFKKNFFLFIEQLKGINATDHILNLETKGISNTITFMEKDSDDIVFLKNELFKLARNVANRAVNNNYECRCVEVLIRKQDKK", "aa_hexdigest": "a4f5ec5a1e87eb5a864edf0788928c10", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "dinP", "mucB" ], "truncated": "3-prime", "pseudogene": { "start": 143913, "stop": 145109, "observations": { "mutation": [ 144804, 144891 ], "stop": [ 144804, 144891 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7R2", "query_cov": 1.0, "subject_cov": 0.7105263157894737, "identity": 0.993, "score": 584.0, "evalue": 7.08e-209, "gene_start": 1, "gene_end": 297, "reference_start": 1, "reference_end": 297, "reference_sequence": "MNKIIFHIDMDSFFVSCERSINTNLIDKPVVIAKRYKRSIISAMSYEVKNLGYRVGSSLYTVLEKVPNLLIVEPHYDLYSLISKKIFDYLKNKYCTQLEIYSIDECYIDFSNTVSNWAEAKNLAQQIKDDILKTFMIPCSIGISFTKFLAKMSTNKAKPSGILITKKGDIERNFYSLPVEKIFGVGKNISQQLIKNKISTYKDLLECNNEIFLRKLFKKNFFLFIEQLKGINATDHILNLETKGISNTITFMEKDSDDIVFLKNELFKLARNVANRAVNNNYECRCVEVLIRKQDKKWMSEQKKIDFFTNDIDIIFKIALKLFLNLWNEKECLRGLGIRICDLRSIFQENKQVNLFEDNNQKSIVNSILNEVNTKFEGKILITGKQYLLENSIDIDNIKFLRKNNQPYDKKINLEEKK" }, "paralog": false, "identity": 0.9824120603015075, "score": 771.0, "evalue": 7.24e-285, "description": "Internal stop codon at 144804, 144891. Nonsense mutation around 144804, 144891.", "psc": { "uniref90_id": "UniRef90_D1J7R2", "gene": "DinP", "product": "DNA-damage repair protein MucB", "uniref50_id": "UniRef50_D1J7R2", "cog_id": "COG0389", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7R2", "UniRef:UniRef50_D1J7R2", "COG:COG0389", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7R2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7R2" ], "product": "DNA-damage repair protein MucB" } }, "id": "AHCKFKLKGB_296", "locus": "GCA013367975_00261" }, { "type": "cds", "sequence": "contig_1", "start": 144939, "stop": 145169, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGTCGATATTTCAAGAAAACAAACAAATCGATTTGTTTGAAGATAATAATCAAAAATCAATAGTAAATTCAATATTAAATGAGGTTAATGCAAAATTTGAGGGTAAAATTTTAATAACGGGGAAACAATATTTGTTAGAGAATTCAATCGATATTGATAATATAAAGTTTTTAAGAAAAAACAATCAACCATATGATAAAAAAATAAATTTAGAGGAGAAAAAATAA", "aa": "MRSIFQENKQIDLFEDNNQKSIVNSILNEVNAKFEGKILITGKQYLLENSIDIDNIKFLRKNNQPYDKKINLEEKK", "aa_hexdigest": "3ba406ffc4b0bbcfda369fa81aaaba84", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9015.179199999991, "isoelectric_point": 7.9263395309448255 }, "id": "AHCKFKLKGB_297", "locus": "GCA013367975_00262" }, { "type": "cds", "sequence": "contig_1", "start": 145169, "stop": 145510, "strand": "+", "frame": 2, "gene": null, "product": "nicotinate-nucleotide adenylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "nt": "ATGAAGATAGGAATATTTGGCGGAAGTTTTGATCCTATTCATAAAGGACATATTTTAATTGCTAATGATGCAATTGAATTATTAAAACTAGATAAAGTTATTTTTGTTCCTGCTAACAAAAATCCTTTTAAAGATAAGCAAGATTATGCATCTAATGAACATAGAATAAATATGATTAACATTGTTATAAACAAGAGTAATATGAAAGTTTCACAATTTGAAACAAAAAGAGGTGGCACTTCATACACAATTGATACTGTAAAATATTTTGCTCAAAAGTATCCAAACGATGAGCTATATTTTTTAATTGGATCGGATAACGTTGGTAGTTTAAACAAATGA", "aa": "MKIGIFGGSFDPIHKGHILIANDAIELLKLDKVIFVPANKNPFKDKQDYASNEHRINMINIVINKSNMKVSQFETKRGGTSYTIDTVKYFAQKYPNDELYFLIGSDNVGSLNK", "aa_hexdigest": "262d6fa00ff26454e4cc192e1b01c021", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FBE0", "query_cov": 1.008849557522124, "subject_cov": 1.0178571428571428, "identity": 0.588, "score": 129.0, "evalue": 7.53e-37, "valid": false, "gene": "nadD", "product": "nicotinate (nicotinamide) nucleotide adenylyltransferase", "ec_ids": [ "2.7.7.18" ], "uniref50_id": "UniRef50_A0A432FBE0", "go_ids": [ "GO:0000309", "GO:0004515", "GO:0005737", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FBE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "product": "nicotinate-nucleotide adenylyltransferase" }, "genes": [], "id": "AHCKFKLKGB_298", "locus": "GCA013367975_00263" }, { "type": "cds", "sequence": "contig_1", "start": 145934, "stop": 146098, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACACCAAACCACGGCCTATTATTGGTTGAGAGATGTATATAAATATCAAAATGATGAAGCGTTACAAGCAATCAAAATTCATACATCATTAGATTTTAAAATATCTTTATTGGCAAAAATTTTATATATTGCTGACAAAATTTGTGAAGGAAGAAAATGA", "aa": "MLHQTTAYYWLRDVYKYQNDEALQAIKIHTSLDFKISLLAKILYIADKICEGRK", "aa_hexdigest": "bd5763502d0e2b7603ea2617461d9da3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6434.442399999998, "isoelectric_point": 8.788862037658692 }, "id": "AHCKFKLKGB_299", "locus": "GCA013367975_00264" }, { "type": "cds", "sequence": "contig_1", "start": 146317, "stop": 147006, "strand": "+", "frame": 1, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0006413", "RefSeq:WP_012855404.1", "SO:0001217", "UniParc:UPI0001BC20F0", "UniRef:UniRef100_D1J7R4", "UniRef:UniRef50_A0A448ZXX5", "UniRef:UniRef90_D1J7R4" ], "nt": "TTGCTGGGTTGCTACAATAAAGAATTTGTAGTTTTTGGGAAGTGGATTAATACCACTTTTTTATTTATAAAAGAAGGAGGAGTTATTATCAATAACCAAAAAATTGGAGACGATTTAAATAACAATCAAACAAATCGTAAACCAAAGTCAGAACATGTTATCAACAATGCTATTCCATACAAAAAAGTTTTTGTTCTAGGACCTAGTGGGGAAAAAATTGGTGTGTTAACCAAAGAAGAAGCTTTAGAAAAAGCATCTGAATATAATATGGATTTAGTTTTAATTGCAATAGAAAATAATAAGCCAATAGTTAGAATATTAGATTATGGCAAATTTAAATATGATAAGAAAAAACGCCAAAAAGAAGTTAAAGAAAAACAAGCAGTTACTGTTAACAGAGAAATTCGCTTAACTCCATTGATTGGTGATCATGATTTGAAAACTAAGGCAAACAAAACTAGAGAATTTATTTTAGATGGTAATAGAGTTAAAGTTTCAGTTAAGTTTCGTGGTAGAGAAAGAGCAAGAATTGAATTAGGGGAAGAAATATTAAAAAAGTTCTTTGCCTTAATTGAAGATGTTGCAAAAATTTCAAAAGAAGCAACATTAGTTAATGATAGATTTTTAGATATGTATGTAGAAAAAGATAAACGAAAAGTTGATACATTAACCAATAAAAAAGATAACTAA", "aa": "MLGCYNKEFVVFGKWINTTFLFIKEGGVIINNQKIGDDLNNNQTNRKPKSEHVINNAIPYKKVFVLGPSGEKIGVLTKEEALEKASEYNMDLVLIAIENNKPIVRILDYGKFKYDKKKRQKEVKEKQAVTVNREIRLTPLIGDHDLKTKANKTREFILDGNRVKVSVKFRGRERARIELGEEILKKFFALIEDVAKISKEATLVNDRFLDMYVEKDKRKVDTLTNKKDN", "aa_hexdigest": "b02804fc375a2cd456cd5400c98cc448", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC20F0", "ncbi_nrp_id": "WP_012855404.1", "uniref100_id": "UniRef100_D1J7R4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20F0", "RefSeq:WP_012855404.1", "UniRef:UniRef100_D1J7R4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7R4", "uniref90_id": "UniRef90_D1J7R4" }, "psc": { "uniref90_id": "UniRef90_D1J7R4", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_A0A448ZXX5", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXX5" ], "product": "Translation initiation factor IF-3" }, "genes": [ "infC" ], "id": "AHCKFKLKGB_300", "locus": "GCA013367975_00265" }, { "type": "cds", "sequence": "contig_1", "start": 147033, "stop": 147221, "strand": "+", "frame": 3, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_020002688.1", "SO:0001217", "UniParc:UPI00036BF9D9", "UniRef:UniRef100_A0A2K9YT13", "UniRef:UniRef50_P75447", "UniRef:UniRef90_D1J7R5" ], "nt": "ATGCCAAAAATGAAAACCAAAAGCGGTTTGAAAAAAAGAATTTCAATCACTGCATCAGGAAAAGTAAAACGTGGACAAGCGTTTAGATCACATTTAGCACAAAACAAAACAACAAAACAAAAACGTCACGCTCGTAAAGCAACAACTTTATCAAGTTCAGATTTTAAAAGATATAAAGAATTAATTTAG", "aa": "MPKMKTKSGLKKRISITASGKVKRGQAFRSHLAQNKTTKQKRHARKATTLSSSDFKRYKELI", "aa_hexdigest": "12cf694fb7c510f41385e8bf4b0e3d29", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00036BF9D9", "ncbi_nrp_id": "WP_020002688.1", "uniref100_id": "UniRef100_A0A2K9YT13", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036BF9D9", "RefSeq:WP_020002688.1", "UniRef:UniRef100_A0A2K9YT13" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YT13", "uniref90_id": "UniRef90_D1J7R5" }, "psc": { "uniref90_id": "UniRef90_D1J7R5", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_P75447", "cog_id": "COG0291", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P75447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75447" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "AHCKFKLKGB_301", "locus": "GCA013367975_00266" }, { "type": "cds", "sequence": "contig_1", "start": 147492, "stop": 147593, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGTAAATAGAAAAATGTTATCTGAATTAGCAATTAATTATCCTACATCATTTGCTAAATTAGTAGATCAAGCAAAATCAGCTTTAGCAAAAAAATAA", "aa": "MLVNRKMLSELAINYPTSFAKLVDQAKSALAKK", "aa_hexdigest": "30ec095d133853a43f24cd1b5e60831b", "start_type": "GTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3680.3854, "isoelectric_point": 9.997450828552246 }, "id": "AHCKFKLKGB_302", "locus": "GCA013367975_00267" }, { "type": "cds", "sequence": "contig_1", "start": 147692, "stop": 147802, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGAAAAAGAGAGTGGTATTAGGAATGAGTGGCGGAGTTGATTCTAGTGTTTGTGGATATTTGCTACAAAAACAAGGATATGACGTTGTTGGTTTGTTCATGAGAAATTGA", "aa": "MKKRVVLGMSGGVDSSVCGYLLQKQGYDVVGLFMRN", "aa_hexdigest": "09399c4b20bbd243650b061f6885b900", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 23, "aa_cov": 0.6388888888888888, "hmm_cov": 1.0, "evalue": 1.2134486653994353e-07, "score": 26.389699935913086, "start": 3, "stop": 25 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 34, "aa_cov": 0.9444444444444444, "hmm_cov": 1.0, "evalue": 4.213490352403422e-12, "score": 41.443851470947266, "start": 3, "stop": 36 } ], "seq_stats": { "molecular_weight": 3936.6466, "isoelectric_point": 9.514002418518068 }, "id": "AHCKFKLKGB_303", "locus": "GCA013367975_00268" }, { "type": "cds", "sequence": "contig_1", "start": 148088, "stop": 148804, "strand": "+", "frame": 2, "gene": null, "product": "tRNA-uridine 2-sulfurtransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E4E9" ], "nt": "ATGGGGCATTATGCAAATGCAATTGACGGAGAATTATTTCGGGCGCAAGATGATAATAAAGACCAATCTTACTTTTTAGCGCAATTAAGTCCTGACCAACTTAAAAAAGTAATTTTTCCATTGGCTAATATTACCAAACCAGAAGTACGAGAAATTGCAAAACAATTAAACTTGTCAACCGCTATGAAAAAAGATTCAACAGGAATTTGCTTTATAGGTGAAAGACACTTCACAAAATTTTTAGAAAATTATATTCCTGCTCAACCAGGCAATATTATTGATATATTTAGCGGGAAAGTGGTTGGAAAACATATTGGTGCGATGTATTACACTATAGGCCAAAGAAAAGGTTTAAATCTTGGCGGGTATGAAAAACCATATTTTGTAGTAGGACATAATCTTTCAAAAAAAGAAATTTATGTAACAAACGATGATCATATTGACTATTTAATGTCAAATAAATTAATTGCAACAAATTTTAATCAAATTTCAAAAGAATTTAATTTTGCTAATTTAACTGCTAAGTTTCGTTATAGACAAAAGGATATCAAAATAACTTTAAAAATTTTAGATGATAATAAAATAGAAGTTTGCTATCCAAATACCGAAGCAGTAACTCCTGGCCAACAAGTTGTAATATATGATGGAAATAAAGTAGTTGGTGGAGCGATAATTGACAAAATTTATTTAAATAATGAATTAAAAACGTTTTTATAA", "aa": "MGHYANAIDGELFRAQDDNKDQSYFLAQLSPDQLKKVIFPLANITKPEVREIAKQLNLSTAMKKDSTGICFIGERHFTKFLENYIPAQPGNIIDIFSGKVVGKHIGAMYYTIGQRKGLNLGGYEKPYFVVGHNLSKKEIYVTNDDHIDYLMSNKLIATNFNQISKEFNFANLTAKFRYRQKDIKITLKILDDNKIEVCYPNTEAVTPGQQVVIYDGNKVVGGAIIDKIYLNNELKTFL", "aa_hexdigest": "881a1a6eed27e99860e8cd4827a0cd23", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030845807", "query_cov": 0.8235294117647058, "subject_cov": 0.98989898989899, "identity": 0.515, "score": 198.0, "evalue": 1.06e-60, "valid": false, "product": "aminomethyltransferase beta-barrel domain-containing protein", "uniref50_id": "UniRef50_A0A380E4E9" }, "pscc": { "uniref50_id": "UniRef50_A0A380E4E9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E4E9" ], "product": "tRNA-uridine 2-sulfurtransferase" }, "genes": [], "id": "AHCKFKLKGB_304", "locus": "GCA013367975_00269" }, { "type": "cds", "sequence": "contig_1", "start": 149082, "stop": 149228, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGGTAACTTTTCAATAGGCGATTATTTTAAAAAAGAAGCAATTGCTTTTGGATATGAATTTATTTTTAATGTTCTAAAACTAAATAAACAAAATATTTATATAACTTATTATTCAGAAGATAAAGACACTTATAATTATTGA", "aa": "MLGNFSIGDYFKKEAIAFGYEFIFNVLKLNKQNIYITYYSEDKDTYNY", "aa_hexdigest": "ee4df62ca45ebba9d68af99bee4f848e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5793.464500000002, "isoelectric_point": 4.992987251281736 }, "id": "AHCKFKLKGB_305", "locus": "GCA013367975_00270" }, { "type": "cds", "sequence": "contig_1", "start": 149520, "stop": 150398, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATGCTCCAACTAATTTTGATACTGACTTATTTTTACCAATAATTCATGCAACAGAAAAATTAACAAACAAACATTATGATATTAATAACTATTTTACAAAAGAACCAATTCAAATGAAAATTAACACCCAATTTAAAATAATTGCTGATCACATTAGAGCTTCAGTTAATGCAATAAGTGACGGTGTTAAACCTTCAAATATTGGTCGTGGTTATATTATTAGACGTCTAATAAGAAGAGCATATAGGGCAGGTATTTCTCTTGGCATAACTCAAAAAGCATTTTTATATGAACTTGTTCAAATAGTTAAAGATTCATTAATTTATGACATTGATGTTGAAGAAGTTAAAAAAGTTATTTTAGATGAAGAAAATCTTTTTGCTAAGACTATTAATGAAGGTGAAAAACTATTAAATCAAGAAATTGAAAAAAATGGTACCATCGATGTTAAAATAGCCTTTAAAATGTTTGACACATATGGCTTTCCTATTGAACTAACAAGAGAAATTCTACAAGAAAAAAACATTGACATCGATATGAATGAATTTAACGAATTTTTAAAGCAACACCAAGAAAAATCAAGAGGAAATATTGAAGCCGGAATGCAAAAAGTTATTAATTCACTTTCATTAATAACTGATAAGATATCTGAATTTGTGGGATATGATCACACTAAAACAACTTCAAAAATTTTATATTTATTAAATAGCGAAGAAAAAATAGATAATGCGGGCGAAGACGAAGTTTCTTATTTAATATTAGATAAAACTCCTTTCTATGCTACAAGTGGTGGCCAAAATCATGATGAAGGTTACATTTTACAAGGAAATAATAGAATTGAAGTTTTAGATGTATTTAAAGACAAATATTGA", "aa": "MQDAPTNFDTDLFLPIIHATEKLTNKHYDINNYFTKEPIQMKINTQFKIIADHIRASVNAISDGVKPSNIGRGYIIRRLIRRAYRAGISLGITQKAFLYELVQIVKDSLIYDIDVEEVKKVILDEENLFAKTINEGEKLLNQEIEKNGTIDVKIAFKMFDTYGFPIELTREILQEKNIDIDMNEFNEFLKQHQEKSRGNIEAGMQKVINSLSLITDKISEFVGYDHTKTTSKILYLLNSEEKIDNAGEDEVSYLILDKTPFYATSGGQNHDEGYILQGNNRIEVLDVFKDKY", "aa_hexdigest": "46e1ec8ddab81e6bf47c0b1d63a53aef", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33614.78540000003, "isoelectric_point": 5.151113319396971 }, "id": "AHCKFKLKGB_306", "locus": "GCA013367975_00271" }, { "type": "cds", "sequence": "contig_1", "start": 150510, "stop": 151484, "strand": "+", "frame": 3, "gene": null, "product": "Alanine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZR4" ], "nt": "ATGTTTAGTGCATTAAGAGAAACATATCCAAACGAAATTATCAAGCAATTAGGTTCGAATATTACATATGAACATTTTACATTTGATTTCGGTCTTGATCATAGGCCAACTAAAGAAGAGATAAAATTAATTGAACATAAAATGAGGCAATATATTGCTAGAGATGTAAAACGTAATTACATAATAACTTCGCTTAAAAAAGCAGAAGAAATGGGCGCATACATGACAATTGATGAATCAGAATATCATGATGCAAATTATATAAGAGTTGTTGAATTTGAAGGTATTACCAAAGATTTATGTGGTGGAACACACGTTCCTCATTCAGGTGTAATTGAAGATTTTAAAATTGTTGCCGTTGATTCAAAAGGTACAGGAATATATAGACTTAGAGTAGTTACTTCATATGATTTAGTAAGAAAATATTTATTATCAGAAATTCAAGAAAAACAAAAAATATTGGATACATTAATTAGAAATAATCAAAAACTAAATGAAGGCTATAACTTTAAATATACAAAGGAAATTATTTCTAATAAGACATCTGATTTAGAAGAATTACTTGAAGAATATGATAAATGCGAAGAACAACTAAGAAATGATTATCGAACATTATTGAAACAAAAACAATCAGAAGATGTTGATTTAAGTTTGTATAATTATGAAACCATCAACGATAAAAAAGTATTCTTATTAATTAACATAGAACAATCATTAATTAAAAAAGTTTCAATCTCATTAAGAGAAGCAAATCCTGATTCAATTATTATTATCTTAAACAAGAGCAAACAAGATTCATATTATTTTGCAGTAGCATCTAAAAAATATGATTCAAATGCAATATTTAAAAACTTGGGCAAGGTTTTAAATATTCATGGCGGTGGAAATGCAATAATTTCTCAAGGTTCATTGGTTTCAAACTTGAAACAAGAAGAAATTATTGAAATTATTAAGAGCAATTTATGCGAATTTTAG", "aa": "MFSALRETYPNEIIKQLGSNITYEHFTFDFGLDHRPTKEEIKLIEHKMRQYIARDVKRNYIITSLKKAEEMGAYMTIDESEYHDANYIRVVEFEGITKDLCGGTHVPHSGVIEDFKIVAVDSKGTGIYRLRVVTSYDLVRKYLLSEIQEKQKILDTLIRNNQKLNEGYNFKYTKEIISNKTSDLEELLEEYDKCEEQLRNDYRTLLKQKQSEDVDLSLYNYETINDKKVFLLINIEQSLIKKVSISLREANPDSIIIILNKSKQDSYYFAVASKKYDSNAIFKNLGKVLNIHGGGNAIISQGSLVSNLKQEEIIEIIKSNLCEF", "aa_hexdigest": "a1737523d4fd836785422373f4b91337", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NZR4", "query_cov": 0.9907407407407407, "subject_cov": 0.9042253521126761, "identity": 0.5579999999999999, "score": 352.0, "evalue": 1.03e-117, "valid": false, "product": "Alanine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0NZR4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NZR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZR4" ], "product": "Alanine--tRNA ligase" }, "genes": [], "id": "AHCKFKLKGB_307", "locus": "GCA013367975_00272" }, { "type": "cds", "sequence": "contig_1", "start": 151472, "stop": 151819, "strand": "+", "frame": 2, "gene": null, "product": "Putative pre-16S rRNA nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM70" ], "nt": "ATGCGAATTTTAGCATTGGATTTAGGCACAAAAAGTTGTGGCTTTGCAATAAGTGATCCTAATAATATAATTGCAAGTGGACTTGAAAATTTTAGATTTGAATTGAATCATTTTAACTTAGTTATTGAAAAAGTGCTACATTATTTAAATAAAAGTGAATATCAAGGTTCAATTGGCACAATAGTTTTAGGATATCCCTTAAGAATGGATTTATCAAAAAGTCAACGAACATATATGGTTGAAAGATTTGGAGAAAGATTAAAAAAAGTTGTTGATATTCCAGTTGTATTTCAAGATGAAAGACAAAGCACTATTGGAGCTCAAGAAATATTGCAGAGCGCTGGATGA", "aa": "MRILALDLGTKSCGFAISDPNNIIASGLENFRFELNHFNLVIEKVLHYLNKSEYQGSIGTIVLGYPLRMDLSKSQRTYMVERFGERLKKVVDIPVVFQDERQSTIGAQEILQSAG", "aa_hexdigest": "6985cc6d5db1c0ac36561d19f3492d67", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00112C3C79", "query_cov": 1.0, "subject_cov": 0.8098591549295775, "identity": 0.687, "score": 160.0, "evalue": 9.65e-49, "valid": false, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_B3PM70", "go_ids": [ "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM70" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [], "id": "AHCKFKLKGB_308", "locus": "GCA013367975_00273" }, { "type": "cds", "sequence": "contig_1", "start": 151899, "stop": 152210, "strand": "+", "frame": 3, "gene": null, "product": "DUF1292 domain-containing protein", "db_xrefs": [ "RefSeq:WP_012855410.1", "SO:0001217", "UniParc:UPI0001BC20F6", "UniRef:UniRef100_D1J7S0", "UniRef:UniRef50_D1J7S0", "UniRef:UniRef90_D1J7S0" ], "nt": "ATGAATAAAAATATTATTAATTTAGATGTAGTTGATAGGCAATTGACTACAAGCGATGGCGAAAAACTTTATGTTATTTTTGATATTGAAGAAAACGGCGAACATTATCTTGTTTTAACAGATTATGACGCGATTATTTTTGCTAAAGAACAAGATCAAAATTTAATCGAAGTAACAGATGAAGGTGAAATTGACATTCTTGTTGATTTGACAATGGAATTTGCAGAAAATAATTTTGTTTTAGATAAAGACGGAAAATCAGATTTAATGAAGAAATTAATTGGCAACGATCAGGGTGAAAATGAAGCATAA", "aa": "MNKNIINLDVVDRQLTTSDGEKLYVIFDIEENGEHYLVLTDYDAIIFAKEQDQNLIEVTDEGEIDILVDLTMEFAENNFVLDKDGKSDLMKKLIGNDQGENEA", "aa_hexdigest": "85120146fd2db4f4c4dc4a506d74e8d6", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC20F6", "ncbi_nrp_id": "WP_012855410.1", "uniref100_id": "UniRef100_D1J7S0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20F6", "RefSeq:WP_012855410.1", "UniRef:UniRef100_D1J7S0" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S0", "uniref90_id": "UniRef90_D1J7S0" }, "psc": { "uniref90_id": "UniRef90_D1J7S0", "product": "DUF1292 domain-containing protein", "uniref50_id": "UniRef50_D1J7S0" }, "pscc": { "uniref50_id": "UniRef50_D1J7S0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S0" ], "product": "DUF1292 domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_309", "locus": "GCA013367975_00274" }, { "type": "cds", "sequence": "contig_1", "start": 152200, "stop": 152919, "strand": "+", "frame": 1, "gene": null, "product": "BC85_0335 family putative methyltransferase", "db_xrefs": [ "RefSeq:WP_114119111.1", "SO:0001217", "UniParc:UPI000DEDE5A6", "UniRef:UniRef100_UPI000DEDE5A6", "UniRef:UniRef50_D1J7S1", "UniRef:UniRef90_D1J7S1" ], "nt": "ATGAAGCATAAGGGTTCAATATTTAGCGCTAATACAAAATTAGGTTTAATAATCTCTGTCGTCGTTGTATTTATTTTAATGTGTGTAATTTTGTTAATAACTATTTTAGCACGTAGACATTTCTTAAAAAGCACAATAACATCAAGCGAAGAACGAGAACTACAAAATTTAAAAACAAAAAATCCAAATTATGGAATTGTTTTGAGTGGCATTAAAAAATATTATGATAATGTTTTAGGCGACCATTTGATTGCGTTTTTAATTAATACAATATATTTGAATAATTTTCTAAACGTTAGTCTTATTGAAAAAAATGATTATGCTTCAATTTCAATAGCAGCATTATCTATGGCTAATGTTTATTCAAAAAATCAATATTTAATTCAAAAGCAAGGTTCAAATATTCAAAACGAAAATCCAGATTTAGATCTATCTAGATTAAATTATGTTGATGAAATTTATAATCCTTCGGATATGATAATATCTTTATGCGATGATCTTTCAATAGAACAACAAATTAATTCAAATTTACCTTATTTAAGTGATAGAGGTATGCTAATTGTTTCATTTAAGACTATAAAAGACTTTAAAAATCAAAAAAATGTTATTTTAGATAATAATTTAAGATACGAAACTGTTAAAATTAGTAATAAAAATGTTATACTTTTAGCAAAAAACAATATGAATTCAAACAATGAAAATTCTAAGGAGCAACAATAA", "aa": "MKHKGSIFSANTKLGLIISVVVVFILMCVILLITILARRHFLKSTITSSEERELQNLKTKNPNYGIVLSGIKKYYDNVLGDHLIAFLINTIYLNNFLNVSLIEKNDYASISIAALSMANVYSKNQYLIQKQGSNIQNENPDLDLSRLNYVDEIYNPSDMIISLCDDLSIEQQINSNLPYLSDRGMLIVSFKTIKDFKNQKNVILDNNLRYETVKISNKNVILLAKNNMNSNNENSKEQQ", "aa_hexdigest": "a2b806deecaa2278c42af202d7081a14", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000DEDE5A6", "ncbi_nrp_id": "WP_114119111.1", "uniref100_id": "UniRef100_UPI000DEDE5A6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DEDE5A6", "RefSeq:WP_114119111.1", "UniRef:UniRef100_UPI000DEDE5A6" ] }, "ips": { "uniref100_id": "UniRef100_UPI000DEDE5A6", "product": "BC85_0335 family putative methyltransferase", "uniref90_id": "UniRef90_D1J7S1" }, "psc": { "uniref90_id": "UniRef90_D1J7S1", "uniref50_id": "UniRef50_D1J7S1" }, "pscc": { "uniref50_id": "UniRef50_D1J7S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S1" ] }, "genes": [], "id": "AHCKFKLKGB_310", "locus": "GCA013367975_00275" }, { "type": "cds", "sequence": "contig_1", "start": 152919, "stop": 153389, "strand": "+", "frame": 3, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "COG:COG0782", "COG:K", "GO:0003677", "GO:0032784", "GO:0070063", "RefSeq:WP_012855412.1", "SO:0001217", "UniParc:UPI0001BC20F8", "UniRef:UniRef100_D1J7S2", "UniRef:UniRef50_B3PM73", "UniRef:UniRef90_D1J7S2" ], "nt": "ATGGCTAATAATGATAATAAGATTTATTTAACAGAAGAAAGTTTAAAGCAATATCAAGAAAAATTAAAATATTTACAAGAAGTTGCAAGACCTCAAGTTATTGAAGAAATTAAAGAAGCAAGAAATCAAGGTGACTTGTCAGAAAATGCTGAATATGACTCAGCTCGTGATAAACAAGCTGCTATTGAAAATGAAATTTTAGAAATTCAACATATTCTTGAAAATGTTGAAATTATTAAATCAACATCAACTCATACAATTAGAATTGGTTCAACTGTAAAATTAATAAATATAAACACAAACGAAATAAGCGAAGTTAAAATTGTTGGTGCATTAGATGCCGATCCATTCAACAATAAAATTTCAAATACCTCACCGCTTGCAATTGCATTAAATGGCAAAAAAGTCGGACAAGTGATTGAAGTTGAAGCTCCTAAAAAATATAAAGTTAAAATTGAATCAATTTTATAA", "aa": "MANNDNKIYLTEESLKQYQEKLKYLQEVARPQVIEEIKEARNQGDLSENAEYDSARDKQAAIENEILEIQHILENVEIIKSTSTHTIRIGSTVKLININTNEISEVKIVGALDADPFNNKISNTSPLAIALNGKKVGQVIEVEAPKKYKVKIESIL", "aa_hexdigest": "64b98e0388441a4943fb2bf3806ee693", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20F8", "ncbi_nrp_id": "WP_012855412.1", "uniref100_id": "UniRef100_D1J7S2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20F8", "RefSeq:WP_012855412.1", "UniRef:UniRef100_D1J7S2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S2", "uniref90_id": "UniRef90_D1J7S2" }, "psc": { "uniref90_id": "UniRef90_D1J7S2", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_B3PM73", "cog_id": "COG0782", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM73" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "AHCKFKLKGB_311", "locus": "GCA013367975_00276" }, { "type": "cds", "sequence": "contig_1", "start": 153494, "stop": 154168, "strand": "+", "frame": 2, "gene": "trmN6", "product": "DNA methylase", "db_xrefs": [ "COG:COG4123", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A448ZXG4", "UniRef:UniRef90_D1J7S3" ], "nt": "ATGAAAAATGAAGTTGTTAAAAATAATTTAGGATATAGCGGAGATTTATTTGTATATCAAGATAAATCAATGTTTAATTATTCTGTTGATACTATTTTACTCGGCAATTTTGTATCAATAAACAGAAAAGTAAGAAATATTTTGGAAGTAGGCACGAATAATGGTGCCCTTTCTATTTTTATTGCTGCAAGAGATAAACGTTTGAACATTGATGCTATTGAAATCCAAAGCAAAGCTGTTGAGCTTGCAAGAAAAAATATTATTTTAAACAAACTTGAAAATCAAATTAATCTTATTGAAGGCGATTTTCGGATGTGGGCTAATCAAAAGGCATACGACTGTGGTAATAAATTAGCCAAAAAATACGATTTAATTGTTGCTAATCCGCCTTACTACAATGAAGATTATAATCAACAAAGAAAAAACTCAACTCTTGAACAAAAACTAGCAACTCATGAAATAAATTTAAATTTAGAACAATTAGTTGCTAATAGTGCAAGAATTATTGAACAAAAAGGATACTTAGCAATTGTTTTGCCAATGGCAAGATATATTGATCTAATTTGTTTGCTAAGAAAATACAATTTTGAACCAAAAAGAATTCAATTAGTTTATCCAAGAATCACTGATTTACCAAAATTCTGTTTAGTTGAAGCCAGGTTTAATTCAGGTTGA", "aa": "MKNEVVKNNLGYSGDLFVYQDKSMFNYSVDTILLGNFVSINRKVRNILEVGTNNGALSIFIAARDKRLNIDAIEIQSKAVELARKNIILNKLENQINLIEGDFRMWANQKAYDCGNKLAKKYDLIVANPPYYNEDYNQQRKNSTLEQKLATHEINLNLEQLVANSARIIEQKGYLAIVLPMARYIDLICLLRKYNFEPKRIQLVYPRITDLPKFCLVEARFNSG", "aa_hexdigest": "1bdc4b0dbaa34b36576f4f70f66f78e0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7S3", "query_cov": 1.0, "subject_cov": 0.8615384615384616, "identity": 1.0, "score": 442.0, "evalue": 1.38e-156, "valid": true, "gene": "TrmN6", "product": "DNA methylase", "uniref50_id": "UniRef50_A0A448ZXG4", "cog_id": "COG4123", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4" ], "product": "DNA methylase" }, "genes": [ "trmN6" ], "id": "AHCKFKLKGB_312", "locus": "GCA013367975_00277" }, { "type": "cds", "sequence": "contig_1", "start": 154299, "stop": 154553, "strand": "+", "frame": 3, "gene": null, "product": "Methionyl-tRNA synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X9AHZ7" ], "nt": "ATGAATAATAATAAAAAGACATTTTATATTACCACACCAATTTATTATCCAAGTGGTAATCTTCACATAGGACATTTATTAACTACTACATTAGCTTCAGTATATAGAAATTACAAAAAACAACAAGGATATGAAACCTTGTTTATTACCGGAATTGATGAACATGGTCAAAAGATTCAAAAGAAAGCCCTTGAACAAAATATTACTCCAAAAGAATATGTAGATATTCAATCTCAAAAGTTCGTTGATTTATGA", "aa": "MNNNKKTFYITTPIYYPSGNLHIGHLLTTTLASVYRNYKKQQGYETLFITGIDEHGQKIQKKALEQNITPKEYVDIQSQKFVDL", "aa_hexdigest": "ab459c6b68362502debb64089bceef2b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00283F72E3", "query_cov": 0.8809523809523809, "subject_cov": 0.8705882352941177, "identity": 0.608, "score": 95.5, "evalue": 3.89e-24, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A7X9AHZ7" }, "pscc": { "uniref50_id": "UniRef50_A0A7X9AHZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X9AHZ7" ], "product": "Methionyl-tRNA synthetase" }, "genes": [], "id": "AHCKFKLKGB_313", "locus": "GCA013367975_00278" }, { "type": "cds", "sequence": "contig_1", "start": 154638, "stop": 154826, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAAAAGGCTATATTTACAAATCTAAATATGAAGGCTTATATTCAATTAGCGATGAAGAATTCTTAACTAAAACTCAAGCAATATCGAAGGATGGAAAATTTTACCACCCAATTTCAGGACACGAACTTCAAGAAGTAAAAGAAGAAAGTTATTTCTTTAATATGTCAAAATTTACCCCTTGA", "aa": "MEQKGYIYKSKYEGLYSISDEEFLTKTQAISKDGKFYHPISGHELQEVKEESYFFNMSKFTP", "aa_hexdigest": "44f2d0256d7935fe5526ee4f0eb1c69b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7354.149999999999, "isoelectric_point": 5.585135841369628 }, "id": "AHCKFKLKGB_314", "locus": "GCA013367975_00279" }, { "type": "cds", "sequence": "contig_1", "start": 154890, "stop": 155006, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTTATTAATAAAGGATTAGAGGATCTTTCGATAACAAGAGTTTCTTTTGACTGGGGAATTAAAATAGAAACTAAAGATGCTGAAAAGCAACACATTATTTATGTTTGA", "aa": "MNNFINKGLEDLSITRVSFDWGIKIETKDAEKQHIIYV", "aa_hexdigest": "2a966a0ca5c85685c2a5f258d4edcb14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4467.0631, "isoelectric_point": 5.564105415344237 }, "id": "AHCKFKLKGB_315", "locus": "GCA013367975_00280" }, { "type": "cds", "sequence": "contig_1", "start": 155232, "stop": 155630, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAATCAAAAGGCAATGTTATTGAACCAATTCCTTTAATTGAAAAATATGGGGCAGAACCCGTTAAGTATTTTTTTGCATCGCAAATAAATATTGACAATGATTTTAGTTTTAGCGAAGAATTACTTATTAATGTATTAAATGCTGATTTAGCAAATAATTTTGGTAATTTGGCCAATAGAACCTTTAAAATGGTAATGTTAAATTTTGAAAATGGTACAACTTATTGTTCAAACCAATTGAATTCATTAGATAAAAATGTTTATTCAGAAATAGAAAATGCATACAATGATTATAAAAAATATTTTGACAATTTTAATGCTGATAAGGCTTTAAAAAGGGCAATTGAATTAAGTAAGTTTTTAAATGAATATATTGATAAAAATGAACCTTGA", "aa": "MSKSKGNVIEPIPLIEKYGAEPVKYFFASQINIDNDFSFSEELLINVLNADLANNFGNLANRTFKMVMLNFENGTTYCSNQLNSLDKNVYSEIENAYNDYKKYFDNFNADKALKRAIELSKFLNEYIDKNEP", "aa_hexdigest": "75025e337a5353f3901e80d0fb1d920c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15236.911499999984, "isoelectric_point": 4.756821250915528 }, "id": "AHCKFKLKGB_316", "locus": "GCA013367975_00281" }, { "type": "cds", "sequence": "contig_1", "start": 155646, "stop": 155864, "strand": "+", "frame": 3, "gene": null, "product": "Methionyl-tRNA synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PIV2" ], "nt": "TTGCCAAGATTAAATGTGGTTTTAAATACATTGTTGAATGGAATTTATGCAGTTAATATGATGTTAAGCGTTGTTTTAAAAGAAAAATGTAACATTTTATTAAATGATTTGCAATTGTCTGAATTCTCTGAAAAAATGTTGTTTAATTATTCTAAATTTGATAACAAAAAGTTTTCTAAACCTAATATTTTATTTGAAAGGATAAAACCAAATAATTAA", "aa": "MPRLNVVLNTLLNGIYAVNMMLSVVLKEKCNILLNDLQLSEFSEKMLFNYSKFDNKKFSKPNILFERIKPNN", "aa_hexdigest": "ea3934c64676a13e84ccfe772178b09b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PIV2", "query_cov": 0.9444444444444444, "subject_cov": 0.9066666666666666, "identity": 0.5, "score": 63.2, "evalue": 1.13e-11, "valid": false, "product": "Methionyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A3B0PIV2" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PIV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PIV2" ], "product": "Methionyl-tRNA synthetase" }, "genes": [], "id": "AHCKFKLKGB_317", "locus": "GCA013367975_00282" }, { "type": "cds", "sequence": "contig_1", "start": 155885, "stop": 157036, "strand": "+", "frame": 2, "gene": "obgE", "product": "GTPase ObgE", "db_xrefs": [ "COG:COG0536", "COG:DL", "GO:0003924", "SO:0001217", "UniRef:UniRef50_Q4A6N4", "UniRef:UniRef90_D1J7S5" ], "nt": "ATGAAATTTATAGATGAAACAAATGTAATAGTAATAGCTGGCAAAGGTGGCGATGGCATAATTAGTTTCAGAAGAGAAGCTAATGTTGACAAAGGCGGTCCTGATGGTGGCGATGGTGGCGATGGTGGCGATGTTTATTTTGTAGGTGATCCTGGCCAAAATACACTTCTACCATTTTATTATCAAAATAAATTGGTCGCTGAATCAGGAGAAGATGGAAGGCCAAAAAATCAATATGGTGCTGATGGCCAAGATTTAATTGTAAAAGTTCCACTTGGAACAATGGTTTATAATGGCGATGATTTAATAGCCGATGTAGTTAGCCAAGAGAAATACTTAATAGCAAAAGGTGGACACGGTGGCAAAGGAAATCCTAGATTTAAATCTAGTACTAACACTGCTCCTAGAATATGTGAAAATGGTACACCTGGTCAAAAATTTGAACTTCACTTAGTTTTAAAAGTTTTAGCAGATGTTGGCTTTGTTGGAAAGCCTAGTGCTGGCAAGAGCACTATTTTAGGCGAAATATCTAATGCAAAGCCTAAAATTGCAGATTATGATTTTACTACATTGGTTCCTCAACTAGGTTTGGTAAAATATTTTGACAACTCATACGTTGTAGCAGATCTACCAGGACTTATTGAAAATGCTCATTTGGGCAAGGGTCTTGGAATTCAATTTTTAAAGCATATTGAAAGATGTAAAATTATTGCTCATGTTATTGATTTTGGTAGTGAAGAAAAGAATCCAATAGATGATTATGAAATAATTAATAATGAATTAAAATCATATAATTTGTTGCTTGAAAAATTACCACAAGTAGTAATAGCAAACAAAAAAGATCTACCGGCTTTTTCACTAAACTTATCAAAATTTAAAGAAAAATATCCAAATATAAATGTATTTGAATATTCAGCATTAATGGCTGAAAATTTAGATGAATTAAAAGGATGCTTGTTTGAAACATTGCAATTTGCTAAAGATTCAATTGCTGTTCAACAAATTCATGAAAGTCAAAACATTGTAAAAATAACATTAAATAAAAAACCTATTCAAATTAAAAAAATTGATAATGAAACATATGAATTGTTTGGTGATGATATAGATAATATATATAATAAGATACCAGTGATTTCATTAGATAATTTATGA", "aa": "MKFIDETNVIVIAGKGGDGIISFRREANVDKGGPDGGDGGDGGDVYFVGDPGQNTLLPFYYQNKLVAESGEDGRPKNQYGADGQDLIVKVPLGTMVYNGDDLIADVVSQEKYLIAKGGHGGKGNPRFKSSTNTAPRICENGTPGQKFELHLVLKVLADVGFVGKPSAGKSTILGEISNAKPKIADYDFTTLVPQLGLVKYFDNSYVVADLPGLIENAHLGKGLGIQFLKHIERCKIIAHVIDFGSEEKNPIDDYEIINNELKSYNLLLEKLPQVVIANKKDLPAFSLNLSKFKEKYPNINVFEYSALMAENLDELKGCLFETLQFAKDSIAVQQIHESQNIVKITLNKKPIQIKKIDNETYELFGDDIDNIYNKIPVISLDNL", "aa_hexdigest": "6d808d26232c10ae3f7412ca9502556f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7S5", "query_cov": 1.0, "subject_cov": 0.9033018867924528, "identity": 0.992, "score": 739.0, "evalue": 2.35e-268, "valid": true, "gene": "obgE", "product": "GTPase ObgE", "uniref50_id": "UniRef50_Q4A6N4", "cog_id": "COG0536", "cog_category": "DL", "go_ids": [ "GO:0003924" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N4" ], "product": "GTPase Obg" }, "genes": [ "obgE" ], "id": "AHCKFKLKGB_318", "locus": "GCA013367975_00283" }, { "type": "tRNA", "sequence": "contig_1", "start": 157306, "stop": 157382, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 86.0, "nt": "GGGGAAGTAGCTCAGCTTGGTAGAGCACTTGACTTGGGCTCAAGCGGTCGCAGGTTCGAATCCTGTCTTCCTCACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 157323, 157325 ], "id": "AHCKFKLKGB_7", "locus": "GCA013367975_00284" }, { "type": "tRNA", "sequence": "contig_1", "start": 157388, "stop": 157464, "strand": "+", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 78.5, "nt": "GGGGGGGTAGCTCACCTGGCCAGAGCGCCTGCCTTGCACGCAGGAGGTAGAGGGTTCGAGTCCCTTCCTCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 157417, 157419 ], "id": "AHCKFKLKGB_8", "locus": "GCA013367975_00285" }, { "type": "tRNA", "sequence": "contig_1", "start": 157469, "stop": 157545, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 77.9, "nt": "GGCACTGTAGCTCAGTCGGTTAGAGCGTCCGGTTCATACCCGGAAGGTCAAGAGTTCGATTCTCTTCGGTGCTACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 157503, 157505 ], "id": "AHCKFKLKGB_9", "locus": "GCA013367975_00286" }, { "type": "tRNA", "sequence": "contig_1", "start": 157570, "stop": 157645, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 86.1, "nt": "GGACCCATAGCTCAATGGTTAGAGCAACCGGCTCATAACCGGTCGGTTACAGGTTCGAGTCCTGTTGGGTCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "AHCKFKLKGB_10", "locus": "GCA013367975_00287" }, { "type": "tRNA", "sequence": "contig_1", "start": 157648, "stop": 157738, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 74.7, "nt": "GGGCAATTACCCAAGAGGCTGAAGGGAGCAGTCTTGAAAACTGCCAGGGGCTTTACGGCCCGCGGGGGTTCAAATCCCTCATTGCCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 157667, 157669 ], "id": "AHCKFKLKGB_11", "locus": "GCA013367975_00288" }, { "type": "tRNA", "sequence": "contig_1", "start": 157745, "stop": 157820, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 69.0, "nt": "AGCGGAGTAGAGCAGGCGGCAGCTCGTTGGGCTCATAACCCAAAGGTCATTGGTTCAAATCCAATCTCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "AHCKFKLKGB_12", "locus": "GCA013367975_00289" }, { "type": "tRNA", "sequence": "contig_1", "start": 157822, "stop": 157898, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 74.0, "nt": "GGTCCAGTGGTAAAGTGGTTTAATACGCTTCCCTGTCACGGAAGAGATCGCGAGTTCAATTCTCGTCTGGACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 157823, 157825 ], "id": "AHCKFKLKGB_13", "locus": "GCA013367975_00290" }, { "type": "tRNA", "sequence": "contig_1", "start": 157900, "stop": 157975, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 76.9, "nt": "GGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCACTGGTTCGATTCCTGTCGGAGCCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 157933, 157935 ], "id": "AHCKFKLKGB_14", "locus": "GCA013367975_00291" }, { "type": "cds", "sequence": "contig_1", "start": 158063, "stop": 158203, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04193.19" ], "nt": "ATGAATATTAATAGTATAAAAATTGCAATTCAATTATTTGGAATTTTTGGTTCAATTGTAACGATTTGTTTAGGAATTCCACAATTAATTAGATTAAAAAAAACAAAAAACACGGGCAAAGTTAGTTTTGCAGCATTTTGA", "aa": "MNINSIKIAIQLFGIFGSIVTICLGIPQLIRLKKTKNTGKVSFAAF", "aa_hexdigest": "fa5f71c74dafc2ab4e51ccf2c18d046b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04193.19", "name": "PQ-loop", "length": 35, "aa_cov": 0.7608695652173914, "hmm_cov": 1.0, "evalue": 2.9639505099284265e-07, "score": 25.503488540649414, "start": 10, "stop": 44 } ], "seq_stats": { "molecular_weight": 5009.071300000001, "isoelectric_point": 10.595137214660646 }, "id": "AHCKFKLKGB_319", "locus": "GCA013367975_00292" }, { "type": "cds", "sequence": "contig_1", "start": 158360, "stop": 158614, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATCAGGGCTATTGTTGGAATAAGCATTAATCAAGTTATTTCATTAATTTTCTTGTGCTTATTTTTTGTTACGGTATATCAAAAGTTAAAATTTGGTAAATTTTATCCCGATAATTCAATTATTCCTGTTCTTCCGGGTAATGTACAACTTGCATTTGGACTAATATGTCCCGCCTTTACAAGTGCAGCATTCTTCCCTGGATTAATTGTTAGTTTAATTAAAAAAGAAGAAATAAGTTTAAGCCCATGA", "aa": "MLIRAIVGISINQVISLIFLCLFFVTVYQKLKFGKFYPDNSIIPVLPGNVQLAFGLICPAFTSAAFFPGLIVSLIKKEEISLSP", "aa_hexdigest": "4c8d8104e842a8bfd8b0330f48f63585", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9235.093599999995, "isoelectric_point": 9.138925361633301 }, "id": "AHCKFKLKGB_320", "locus": "GCA013367975_00293" }, { "type": "cds", "sequence": "contig_1", "start": 158880, "stop": 159236, "strand": "-", "frame": 2, "gene": "yIH1", "product": "Impact N-terminal domain-containing protein", "db_xrefs": [ "COG:COG1739", "COG:R", "RefSeq:WP_277872615.1", "SO:0001217", "UniParc:UPI0024110DCF", "UniRef:UniRef100_UPI0024110DCF", "UniRef:UniRef50_D1J7S7", "UniRef:UniRef90_D1J7S7" ], "nt": "ATGGCAATTTTAGAAATTAAAAAGTCTAAATTTATTTCTTATAAATTTAATATTAAAAGTAAAGATGATGTGAAACTTTTAAAAGAGCAATTAAAAAATCAACATCAAAAAGCAAGACATATAGTTTATGCTTATTTAGTTATTGAAAATGGCGTTCAATGTGGCGGAATGAGTGATGATGGTGAACCATCAGGAACAGCAGGCAAACCTTTATATTTTTTACTAGAAAGAAATAAATTAAATAATTGTGTGTTGTTTGTAGTTAGATATTTTGGCGGCATAAAATTAGGCGCAAGTAAATTAATTAGAACATATTCTAAAGTTGCAAATGATGAAATTAAAAGTAGCAATCAATAA", "aa": "MAILEIKKSKFISYKFNIKSKDDVKLLKEQLKNQHQKARHIVYAYLVIENGVQCGGMSDDGEPSGTAGKPLYFLLERNKLNNCVLFVVRYFGGIKLGASKLIRTYSKVANDEIKSSNQ", "aa_hexdigest": "af021c8df47111fc90d0b88bbb47a817", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0024110DCF", "ncbi_nrp_id": "WP_277872615.1", "uniref100_id": "UniRef100_UPI0024110DCF", "db_xrefs": [ "SO:0001217", "UniParc:UPI0024110DCF", "RefSeq:WP_277872615.1", "UniRef:UniRef100_UPI0024110DCF" ] }, "ips": { "uniref100_id": "UniRef100_UPI0024110DCF", "uniref90_id": "UniRef90_D1J7S7" }, "psc": { "uniref90_id": "UniRef90_D1J7S7", "gene": "YIH1", "product": "Impact N-terminal domain-containing protein", "uniref50_id": "UniRef50_D1J7S7", "cog_id": "COG1739", "cog_category": "R" }, "pscc": { "uniref50_id": "UniRef50_D1J7S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S7" ], "product": "Impact N-terminal domain-containing protein" }, "genes": [ "yIH1" ], "id": "AHCKFKLKGB_321", "locus": "GCA013367975_00294" }, { "type": "cds", "sequence": "contig_1", "start": 159277, "stop": 160170, "strand": "+", "frame": 1, "gene": "rluA", "product": "Pseudouridine synthase", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.23", "KEGG:K06180", "RefSeq:WP_012855418.1", "SO:0001217", "UniParc:UPI0001BC20FE", "UniRef:UniRef100_D1J7S8", "UniRef:UniRef50_Q4A5G9", "UniRef:UniRef90_D1J7S8" ], "nt": "ATGTTAAAAATTATTTCAAATTATTCAGACAGAATAGATAAATATGTTGCTAATAATAGCGAGATTTCAAGAAACGATATTAAGGAATTAATTGAAAATGGCGCAGTATTTGTAAATTCAATAAAGATAAATAAACCTAAATTCGTTGTTAAAGAAGGCGATGAAATTTTAATTGAAAGATTATTAGATAAACAAATAAAAGTCGAACCTCAAAACATTCATTTAGATATAGTGTATGAATGCGATGATTATTTAATAATTAATAAGCCATCAGGCTTAGTTGTTCATCCGGCTCCTGGACATCATGATAATACATTAGTCAATGCTTTGATGTATCATTTTAAAAATAATTTAAGTGATGTTAATGGTTTATTACGCATGGGGGTTGTTCACAGAATCGATAAAGATACTAGCGGTTTATTATTAATTGCTAAAAACAATAAAGCGCATAACTACTTTGCTTCTCTTTTAAAAACACATGAAATTAAAAGGAGCTATTATGCTATTGTTGAAGGCAAGATTGCAAATGAAGAATTACATATTGAACTTCCAATAGGTAGAGATACTAAAAATCGCCAAAAATATGCTGTTAGTGAAACAAATTCAAAACCTGCTTATACAATAGTAAATGTTGTCAAATATTTTAAAATGAATAATAAAGATTTTTCATTGGTTAAATGTAATTTAAAAACTGGTCGTACTCATCAAATTAGAGTTCACTTAGCATATATTAAACATCCTGTTTATGGAGATCCAATTTACGGTCATAAGATTGATGATTTCAATCAAAGGCTTCATGCATTTGCTTTAGATTTTGTTGATTTAAATGGAAACAATAAACATTTTGAAGCAGAAATGCCTGAAATTATGAAAAAAGAAATAACTGGCGAATAA", "aa": "MLKIISNYSDRIDKYVANNSEISRNDIKELIENGAVFVNSIKINKPKFVVKEGDEILIERLLDKQIKVEPQNIHLDIVYECDDYLIINKPSGLVVHPAPGHHDNTLVNALMYHFKNNLSDVNGLLRMGVVHRIDKDTSGLLLIAKNNKAHNYFASLLKTHEIKRSYYAIVEGKIANEELHIELPIGRDTKNRQKYAVSETNSKPAYTIVNVVKYFKMNNKDFSLVKCNLKTGRTHQIRVHLAYIKHPVYGDPIYGHKIDDFNQRLHAFALDFVDLNGNNKHFEAEMPEIMKKEITGE", "aa_hexdigest": "c66bfbcb51e14849c3255465f1b3c238", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC20FE", "ncbi_nrp_id": "WP_012855418.1", "uniref100_id": "UniRef100_D1J7S8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20FE", "RefSeq:WP_012855418.1", "UniRef:UniRef100_D1J7S8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S8", "uniref90_id": "UniRef90_D1J7S8" }, "psc": { "uniref90_id": "UniRef90_D1J7S8", "gene": "RluA", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.23" ], "uniref50_id": "UniRef50_Q4A5G9", "cog_id": "COG0564", "cog_category": "J", "kegg_orthology_id": "K06180" }, "pscc": { "uniref50_id": "UniRef50_Q4A5G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5G9" ], "product": "Pseudouridine synthase" }, "genes": [ "rluA" ], "id": "AHCKFKLKGB_322", "locus": "GCA013367975_00295" }, { "type": "cds", "sequence": "contig_1", "start": 160715, "stop": 161194, "strand": "+", "frame": 2, "gene": null, "product": "Rho termination factor N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S9", "UniRef:UniRef90_D1J7S9" ], "nt": "GTGGCAACATTGTTATATATAGTTTCATATTTTGCTGTATTTTTAAAATGCTCTTATGTTGCAAAACTATTTTTAATTTTAAAAAGAAATTTAGCATTTAAGGCATTAAACAACTCGGGCTCGATGGATGATATTTTAAGATCATATTTCTATGGTCAAGAATCTCCTGAATCAACACTAAATACCAATAATACATTTGATTCAAATCAAAAAACCGAAGAAAACAAATCTGAAGATTTAAACAAAGAAGAAGATTCAAAATCAATTGAATATCGTAGTAAATTAGAACAATTAAATGATGAACAATTGCAAAATATGGCCAAGAAATTAAATATTTTTGGAATTGAAACATTTTCAAGAGAACAATTGATTTCAAAAATTAGCGATATTTTTGAACAATCAGCCAAGTCTCAAGATCAACAATCAAAACCAAATAATCTTGACGAAGACAACAACGGTGAAGGCCAAACCAACAATTAA", "aa": "MATLLYIVSYFAVFLKCSYVAKLFLILKRNLAFKALNNSGSMDDILRSYFYGQESPESTLNTNNTFDSNQKTEENKSEDLNKEEDSKSIEYRSKLEQLNDEQLQNMAKKLNIFGIETFSREQLISKISDIFEQSAKSQDQQSKPNNLDEDNNGEGQTNN", "aa_hexdigest": "7ab764c574faaa66eb7826937f598467", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 160415, "stop": 161194, "observations": { "mutation": [ 160541, 160688 ], "stop": [ 160541, 160688 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7S9", "query_cov": 0.9937106918238994, "subject_cov": 0.49221183800623053, "identity": 0.956, "score": 229.0, "evalue": 1.92e-72, "gene_start": 2, "gene_end": 159, "reference_start": 164, "reference_end": 321, "reference_sequence": "MKSININEYNSTNELYTKNKKVFWAWFIAWSVLFATILIFVIASFIQFAVEKQNYLNKYAELFGQIQKLNPNFQGDVFALANNEYVKSLAMSGVLLVILIAVYVWHIVSIALVAKNKDFSAYSRILTTLYLFVIIYEIITLIFSGFQSFSLAIWQVNKILNLVATLLYIVSYFAVFLKCSYVAKLFLILKRNLAFKALNNSGSMDDILRSYFYGQESPESTLNTNNTFDSNQKTEENKSEDLNKEEDSKSIEYRSKLEQLNDEQLQNMAKKLNIFGIETFSREQLISKISDIFEQSAKSQNQQSKPDNSDEDNNDGSQTSN" }, "paralog": false, "identity": 0.9652509652509652, "score": 431.0, "evalue": 1.16e-154, "description": "Internal stop codon at 160541, 160688. Nonsense mutation around 160541, 160688.", "psc": { "uniref90_id": "UniRef90_D1J7S9", "product": "Rho termination factor N-terminal domain-containing protein", "uniref50_id": "UniRef50_D1J7S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7S9", "UniRef:UniRef50_D1J7S9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S9" ], "product": "Rho termination factor N-terminal domain-containing protein" } }, "id": "AHCKFKLKGB_323", "locus": "GCA013367975_00296" }, { "type": "cds", "sequence": "contig_1", "start": 161237, "stop": 161332, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATCAAACATCATTAAAACAACATATTTTTACTTTTTTTTCACAAAATTCTCCTGCTAGTCCAATAAAAAATGGTGATCAAAAATTATGA", "aa": "MNNQTSLKQHIFTFFSQNSPASPIKNGDQKL", "aa_hexdigest": "06f405e4343d81d7761327974f8a8dfb", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3521.910500000001, "isoelectric_point": 9.700380516052245 }, "id": "AHCKFKLKGB_324", "locus": "GCA013367975_00297" }, { "type": "cds", "sequence": "contig_1", "start": 161621, "stop": 162076, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGCACTTTCAATTGTTTTAATAATATCAATTAATTTATCACTATTAGCAATTTATTTTGTTTCATTTAAAGAAGCTAAGTTTGTTAATAAAAAAAATTTAGAAGATGAGCTTATTTCTTTGAAGTATAAAAAATCCAAAGAATTAAATGATTTAAATGCTCCTGAAAATATTAAACTAGATATCAAAGCAATGGAATCACACAATCCATTAGCACCTAGAGTGTTAGCAAAATTTATTGATTTTTATAACAAAATGTCAAATATCGAATTATTTAAACGTTATAAAAATTATTTAAATCAAACATTCAAAATTAGATATTTTAATGAATCCCTTGAAGTAATTGAGGGAAAAAATGAAATGGAAGATAATCTTGAAAAATATTTAGACAACATAAATAATCAAGATCCTGAATTGCAAAAAAATTCTCTAATTTTAAAGGAAATTGCTTGA", "aa": "MIALSIVLIISINLSLLAIYFVSFKEAKFVNKKNLEDELISLKYKKSKELNDLNAPENIKLDIKAMESHNPLAPRVLAKFIDFYNKMSNIELFKRYKNYLNQTFKIRYFNESLEVIEGKNEMEDNLEKYLDNINNQDPELQKNSLILKEIA", "aa_hexdigest": "5a7d0f4c87b7b3ef5cf041862fb95f45", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17752.426799999994, "isoelectric_point": 6.621481132507324 }, "id": "AHCKFKLKGB_325", "locus": "GCA013367975_00298" }, { "type": "cds", "sequence": "contig_1", "start": 162077, "stop": 162229, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATATGGATAAAAAGGTAAAAATTATTAGAGAAGCAGATAAAAATATAACCATGTCTAAAAAAGAATTTCAAGAAAAATATGATGAATATGTTTATTTGCAAAGATCACAAGATCCTGCATATCAACAAACTCAAAAGAATACATGA", "aa": "MNDMDKKVKIIREADKNITMSKKEFQEKYDEYVYLQRSQDPAYQQTQKNT", "aa_hexdigest": "01d8f0b425674717c14c6c03c8d4da74", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6119.825400000004, "isoelectric_point": 7.9425855636596685 }, "id": "AHCKFKLKGB_326", "locus": "GCA013367975_00299" }, { "type": "cds", "sequence": "contig_1", "start": 162422, "stop": 163021, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_175933416.1", "SO:0001217", "UniParc:UPI00158B0CF9", "UniRef:UniRef100_UPI00158B0CF9", "UniRef:UniRef50_D1J7T1", "UniRef:UniRef90_D1J7T1" ], "nt": "ATGACTAAGAAACACAAAAGAATTTTATTTTTATCAACATTAGCATTACCCGTAGCCCTTGTACCTGCAACTATGGTTTCGTGTTCAAAAAAAGATATTGAAGAAATAAAATTGGATATGATCACAATAGCAAAAAATCAAATGCTAAATTATTTACAAAAATTTGAATCATTTTTTAAAAATGATGATGATCCTGATGTTAAAAATTTAGAAATAGAATTTAATAAAGTTATAATTTCATTAAAAAAAATGGATATAACACCTGATTTTAATATGGACCAATTTATTCAAGAAATGACTAAAAAATTCAGTGATGTAGCAAATAATTTTTTGAATTTAAAATACAATAAAGCCAAATTGACAAGTGAATATTATATTTCAAAAGTAAAAGCTCAGCTAATTTTGGAGCAATTTAAAAATCCCGGTTTGTTTCCAAAAAAGAACGAATTTGCCAAGATTTTAGAGCAAGAATTATCAAAAGTTTCCGATCCAACTATTAAAACTTCTGAAGAAGAGTTAAAAAATTCAATTAGATTTTTAGATGATTTAAACAAAAAATATGATGAAATTTTAAAAAAGCAAGTAATGATTTTAAAATAA", "aa": "MTKKHKRILFLSTLALPVALVPATMVSCSKKDIEEIKLDMITIAKNQMLNYLQKFESFFKNDDDPDVKNLEIEFNKVIISLKKMDITPDFNMDQFIQEMTKKFSDVANNFLNLKYNKAKLTSEYYISKVKAQLILEQFKNPGLFPKKNEFAKILEQELSKVSDPTIKTSEEELKNSIRFLDDLNKKYDEILKKQVMILK", "aa_hexdigest": "4d8d510768963b52d1aea452705c8e85", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158B0CF9", "ncbi_nrp_id": "WP_175933416.1", "uniref100_id": "UniRef100_UPI00158B0CF9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B0CF9", "RefSeq:WP_175933416.1", "UniRef:UniRef100_UPI00158B0CF9" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B0CF9", "uniref90_id": "UniRef90_D1J7T1" }, "psc": { "uniref90_id": "UniRef90_D1J7T1", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J7T1" }, "pscc": { "uniref50_id": "UniRef50_D1J7T1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T1" ], "product": "Lipoprotein" }, "genes": [], "id": "AHCKFKLKGB_327", "locus": "GCA013367975_00300" }, { "type": "cds", "sequence": "contig_1", "start": 163183, "stop": 163599, "strand": "+", "frame": 1, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02950", "RefSeq:WP_012855422.1", "SO:0001217", "UniParc:UPI0001BC2102", "UniRef:UniRef100_D1J7T2", "UniRef:UniRef50_P92532", "UniRef:UniRef90_A0A858U5X2" ], "nt": "ATGCCTACAGTTGCACAATTAGTAAAACAAGGCCGTAAAGATAAAACAACAAAATCTAAAGCTCCTGCTTTAGGAAAAATGTATAACTCTTTACAAAAAAAAGAAACTGCTATGCCCGCACCATTTAAGCGTGGCGTATGTACTCGTGTGGCAACAATGACACCTAAGAAACCTAACTCAGCTATTAGAAAATATGCCCGTGTTAGATTATCAAATGGTCAAGAAGTTACTGCCTATATTCCCGGTGAAGGACACAATTTACAAGAACACAGTGTTGTTTTAATTCGTGGAGGTAAGGTTAAAGATTTACCCGGAGTTAGATACACAATTGTTCGTGGAACACAAGATGCTGCCGGAGTTAATAACAGAAAGCAAGCTCGTTCTATTTATGGAACTAAAAAACCAAAAAGTAATTAA", "aa": "MPTVAQLVKQGRKDKTTKSKAPALGKMYNSLQKKETAMPAPFKRGVCTRVATMTPKKPNSAIRKYARVRLSNGQEVTAYIPGEGHNLQEHSVVLIRGGKVKDLPGVRYTIVRGTQDAAGVNNRKQARSIYGTKKPKSN", "aa_hexdigest": "f80b852c405717dd77647c74b99dac37", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2102", "ncbi_nrp_id": "WP_012855422.1", "uniref100_id": "UniRef100_D1J7T2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2102", "RefSeq:WP_012855422.1", "UniRef:UniRef100_D1J7T2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7T2", "uniref90_id": "UniRef90_A0A858U5X2" }, "psc": { "uniref90_id": "UniRef90_A0A858U5X2", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "genes": [ "rpsL" ], "id": "AHCKFKLKGB_328", "locus": "GCA013367975_00301" }, { "type": "cds", "sequence": "contig_1", "start": 163630, "stop": 163938, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "nt": "ATGTCAAGAAAACATAAAGCGCCTGTTAGAGATGTCTTAGCAGATCCAGTTTTTAACTCTAAGATAATTACTAAATTAGTAAATACCATTATGTTAGATGGTAAAAAATCTATTGCTGAAAACATTTTATACAGTGCATTTGATATTATTAAAACAAAAACAGGAAAAGAACCAGTTGAAGTATTTAATCTAGCATTAGAAAATGTTAGTCCACAACTAGAAGTTAGATCAAGAAGAGTTGGTGGTTCAAACTACCAAGTTCCTTGTGAAGTTTCTGCAAAAAGAAAACAAACTTTAGCATTAAGATGA", "aa": "MSRKHKAPVRDVLADPVFNSKIITKLVNTIMLDGKKSIAENILYSAFDIIKTKTGKEPVEVFNLALENVSPQLEVRSRRVGGSNYQVPCEVSAKRKQTLALR", "aa_hexdigest": "a846a895e05ab362d597ef3c44d7d680", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A381KMK1", "query_cov": 1.0, "subject_cov": 0.8429752066115702, "identity": 0.5589999999999999, "score": 124.0, "evalue": 1.23e-34, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A381KMK1", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A381KMK1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "product": "Ribosomal protein S7" }, "genes": [], "id": "AHCKFKLKGB_329", "locus": "GCA013367975_00302" }, { "type": "cds", "sequence": "contig_1", "start": 163975, "stop": 164097, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948V4F4", "UniRef:UniRef90_J9ZBC4" ], "nt": "ATGGAAGAAAAGTTAGCAAATGAATTAATAGATGCCTCAAACAAAGTTGGTGGCGCTATTAAGAAACGTGAAGATACCCATAAAATGGCTGAATCTAACAAAGCATTTGCTCACTTTAGATGA", "aa": "MEEKLANELIDASNKVGGAIKKREDTHKMAESNKAFAHFR", "aa_hexdigest": "c45ebf744fe7d637b0f9acfff3932c27", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.775, "score": 69.7, "evalue": 4.19e-15, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 163924, "stop": 164097, "observations": { "mutation": [ 163936 ], "stop": [ 163936 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_J9ZBC4", "query_cov": 1.0, "subject_cov": 0.6896551724137931, "identity": 0.8, "score": 69.3, "evalue": 7.18e-15, "gene_start": 1, "gene_end": 40, "reference_start": 18, "reference_end": 57, "reference_sequence": "MALRWIVDSAYKRAGHSMEEKLAGELLDASNNTGGAVKKREDTHRMAEANKAFAHYRW" }, "paralog": false, "identity": 0.6666666666666666, "score": 79.0, "evalue": 1.12e-21, "description": "Internal stop codon at 163936. Nonsense mutation around 163936.", "psc": { "uniref90_id": "UniRef90_J9ZBC4", "product": "Ribosomal protein S7", "uniref50_id": "UniRef50_A0A948V4F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_J9ZBC4", "UniRef:UniRef50_A0A948V4F4" ] } }, "id": "AHCKFKLKGB_330", "locus": "GCA013367975_00303" }, { "type": "cds", "sequence": "contig_1", "start": 164113, "stop": 164268, "strand": "+", "frame": 1, "gene": "lepA", "product": "GTP-binding protein lepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4", "UniRef:UniRef90_A0A3P8KNQ4" ], "nt": "ATGTCTCGTGAATATAAATTAGAAGATTACCGCAATATCGGAATCATGGCTCACATTGATGCCGGAAAAACCACAACTACTGAACGTGTGTTATATCACACAGGAAAAATTCATAAAATTGGTGAAACACACGAAGGTGCTTCACAAATGGACTGA", "aa": "MSREYKLEDYRNIGIMAHIDAGKTTTTERVLYHTGKIHKIGETHEGASQMD", "aa_hexdigest": "819aa66155ed42a00a7c7201fbcb4d6f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003516DBAA", "query_cov": 1.0, "subject_cov": 0.9622641509433962, "identity": 0.725, "score": 84.7, "evalue": 8.03e-21, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A962BSW7" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 164113, "stop": 164325, "observations": { "mutation": [ 164266 ], "stop": [ 164266 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "query_cov": 1.0, "subject_cov": 0.6891891891891891, "identity": 0.882, "score": 100.0, "evalue": 5.36e-27, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MARQYKLEDYRNIGIMAHIDAGKTTTTERILLHTGKIHKLGETHDGASQMDWMAQEQERGITITSAATNSILKW" }, "paralog": false, "identity": 0.8571428571428571, "score": 126.0, "evalue": 7.62e-40, "description": "Internal stop codon at 164266. Nonsense mutation around 164266.", "psc": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KNQ4", "UniRef:UniRef50_A0A3P8KNQ4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "product": "GTP-binding protein lepA" } }, "id": "AHCKFKLKGB_331", "locus": "GCA013367975_00304" }, { "type": "cds", "sequence": "contig_1", "start": 164530, "stop": 165636, "strand": "+", "frame": 1, "gene": null, "product": "Elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A379SQ57" ], "nt": "ATGGGTGCTAACTTTAAAGCATCTGTTGAATCACTTCATAAATTACTTGGTGCTAATGCTCACGCTATTCAATTAAATATTGGTGAAGAAAACCATTTTGTTGGAATCATTGATTTAGTTGAAATGAAAGCATATTCATATGATGGCGAAGTTGATGAAAACGCTAAAGAAATTGCTATTCCAGAACATTTAAAAGATGAAGCTCATATTATGAGGGCTGAATTAGCTGAATCACTTGTTGATTATGATGAAGAACTTATGACTTTATTATTAGAAGAAAAAGAAGTTCCAGTTGACATTTTAAAAAGAGCAATTAGAACTGCTACAATTTCTTCTGAATATTTCCCAGTTGTTTGTGGAACATCATTTAAAAACAAGGGTGTTAAGTTAATGCTTGATGCAATTGTTGATTATTTACCAAGTCCTTTAGATATTCCTCCAATGAAGGCATATAAAGGCGAATCAGAAATTCATATCCCTGCCTCAGATGATGAATTTTTCTCAGCTTTAGCATTCAAAGTTATGAATGACCCATTTGTTGGAAACCTAACATACTTTAGAGTTTACTCAGGAATTGTTTCAAAAGGTTCTTATGTATTCAATTCAACAAAAGGTGAAAAAGAAAGACTAAGTAGAATTCTATTGATGCATGCTAATAGTCGTACAGACATTGACGAAGTTAGAACAGGAGATATTGCTGCAGCAGTTGGTTTAAAAGTAACAACTACTGGAGATACATTGATTGATGAAAAACAAAAAGATGTTGTATTGGAAAATATGAATTTCCCTGAACCAGTTATATCTCAAGCTATTGAACCAAAGACCAAAGATGCTTCTGAAAAATTATCATTAGCATTGCAAAGATTGGCTGCCGAAGACCCAACATTTAAATACTACACCGATGAAGAAACAGGACAAACAATTATTGCAGGTATGGGTGAACTTCACCTTGACATTATTGTTGACCGTCTAAGACGTGAATTTAAAGTTGAAGTAACAGTTGGCGCACCACAAGTTTCATATCGTGAAACCATTACAAAGACTGCCGATGTTGAAGGTATGCACAAAAAGCAATCTGGTGGTAAAGGTCAATATGGACACGTTTGA", "aa": "MGANFKASVESLHKLLGANAHAIQLNIGEENHFVGIIDLVEMKAYSYDGEVDENAKEIAIPEHLKDEAHIMRAELAESLVDYDEELMTLLLEEKEVPVDILKRAIRTATISSEYFPVVCGTSFKNKGVKLMLDAIVDYLPSPLDIPPMKAYKGESEIHIPASDDEFFSALAFKVMNDPFVGNLTYFRVYSGIVSKGSYVFNSTKGEKERLSRILLMHANSRTDIDEVRTGDIAAAVGLKVTTTGDTLIDEKQKDVVLENMNFPEPVISQAIEPKTKDASEKLSLALQRLAAEDPTFKYYTDEETGQTIIAGMGELHLDIIVDRLRREFKVEVTVGAPQVSYRETITKTADVEGMHKKQSGGKGQYGHV", "aa_hexdigest": "673df4d7d54be95568c1227b72ebd123", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00255A9B97", "query_cov": 1.0, "subject_cov": 1.005464480874317, "identity": 0.503, "score": 335.0, "evalue": 3.36e-110, "valid": false, "product": "translation factor GTPase family protein", "uniref50_id": "UniRef50_A0A379SQ57" }, "pscc": { "uniref50_id": "UniRef50_A0A379SQ57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A379SQ57" ], "product": "Elongation factor G" }, "genes": [], "id": "AHCKFKLKGB_332", "locus": "GCA013367975_00305" }, { "type": "cds", "sequence": "contig_1", "start": 165748, "stop": 166119, "strand": "+", "frame": 1, "gene": null, "product": "Elongation factor G domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AGV8" ], "nt": "ATGGCTATTGGTATTTTAGCAGGATACCCTATGATAGATATCAAAGCTACTTTATTTGATGGATCATACCACGAAGTTGACTCGTCTGAATTAGCATATAAAATTGCTGCTTCTAAATCACTTACAAAAGGCCGTGAAGCCTTGGGAACAGTATTACTAGAACCTATTATGGATGTTGCTGTTGTTGTTCCTGAAGACTTCTTTGGAGATGTTATGGGTGATATTTCTAGAAGAAGAGGGCAAGTTAGAGATAACGAAACAAGAAACGATGGAGCTCATGTTATTAAAGCATATATTCCATTGAGTGAAATGTTTGGTTATGCAACTCAATTACGTTCAATGACTACAGGTCGTGGAACATACCAAATGTGA", "aa": "MAIGILAGYPMIDIKATLFDGSYHEVDSSELAYKIAASKSLTKGREALGTVLLEPIMDVAVVVPEDFFGDVMGDISRRRGQVRDNETRNDGAHVIKAYIPLSEMFGYATQLRSMTTGRGTYQM", "aa_hexdigest": "96f465eb36f63e8aba1a1d4f44085104", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449AGV8", "query_cov": 1.0, "subject_cov": 0.9318181818181818, "identity": 0.772, "score": 194.0, "evalue": 3.83e-62, "valid": false, "product": "Elongation factor G domain protein", "uniref50_id": "UniRef50_A0A449AGV8" }, "pscc": { "uniref50_id": "UniRef50_A0A449AGV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AGV8" ], "product": "Elongation factor G domain protein" }, "genes": [], "id": "AHCKFKLKGB_333", "locus": "GCA013367975_00306" }, { "type": "cds", "sequence": "contig_1", "start": 166465, "stop": 166680, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTCAAGAAACTCGAGATAAATTAATCTTAGCTAATGCCTTGCCTTCTGTTTCAGGTGCAATGTTGCAAGTTAGATTTACATTTACCTACATTAGTAACATTTTAATAGGTTTTGCATTGATATTTTATGGTTTACTTCCTAATTATCTATGGACAAAAAGAGGATTTTTTTTGGCAACTGTTTATATAAATATAACATTCTTTACTTTTTGA", "aa": "MSQETRDKLILANALPSVSGAMLQVRFTFTYISNILIGFALIFYGLLPNYLWTKRGFFLATVYINITFFTF", "aa_hexdigest": "53e70e2870cc45461ed2b56fb7457907", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8180.5798999999915, "isoelectric_point": 9.694836235046388 }, "id": "AHCKFKLKGB_334", "locus": "GCA013367975_00307" }, { "type": "cds", "sequence": "contig_1", "start": 166906, "stop": 167229, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGTAACTAATTTATTTTTAATTGAACAATTTAATAAAAAAACAACTGGCCTTGATATTTTTACAAAAACTAAGGTGGCAATCTATCCCTTTTTAAATTTAAAATATCCTCTTGGCTATCATGGCAATAATTTATTTTATAAAATTTTATTAAATTTATTTATTCCGATATTTGGAATTATTGTATGTATATCTGTTGCTTATTTTTTAAAATTTATTTGTAAGATTCAAACGTATTCAAAAAAAGATTTTGAAAATCCTGCGTTTTTAAGAGAAAATAAGCTCTTAATAAAATCAATAAAATCACAAATTTTTAAGTAA", "aa": "MQVTNLFLIEQFNKKTTGLDIFTKTKVAIYPFLNLKYPLGYHGNNLFYKILLNLFIPIFGIIVCISVAYFLKFICKIQTYSKKDFENPAFLRENKLLIKSIKSQIFK", "aa_hexdigest": "4d283377d9f4a9083156c97c15d29ca5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12580.982599999981, "isoelectric_point": 9.758853340148924 }, "id": "AHCKFKLKGB_335", "locus": "GCA013367975_00308" }, { "type": "cds", "sequence": "contig_1", "start": 167241, "stop": 167870, "strand": "+", "frame": 3, "gene": null, "product": "DUF1846 domain-containing protein", "db_xrefs": [ "COG:COG4868", "COG:S", "SO:0001217", "UniRef:UniRef50_D1J7T6", "UniRef:UniRef90_D1J7T6" ], "nt": "ATGACGGCTTTTAATTTAAAAAAATATTTTAAGTTACAAACAATTGAAATCAAAAATAGAATTAAGCAATTTAACAATAAACTATATTTAGAATTTGGGGGCAAAATTTTTGACGATTTACATGCTTCCAGAGTTTTACCTGGCTTTTTAAGCGATAGCAAATTACAATTATTGTTAAAATTAAAAAACGAAGCAGAAATTGTAGTTGTAATTTCTGCTAATGACATTGAAAACAATAAATTGCGTGGTGATCTAGGAATCAAATATGAAGATGATGTATTAAGATTAATTGATAACTTTAAAAGTTACGATTTAATTGTAAATAATGTAGTTATTACCCAATATCAATCAACCTTTAGAGTTAATAAACTGCAAAACATTCTTAGTGAACATAATATCAATGTCGTTAAACACTATAAGATTGACGATTATCCTAATAATTTAGATTTAATAGTTTCAAAAAATGGATATGGCAAAAATGAAACTATAAAAACTCAAAAGCCATTAATAGTAATTACTGCCCCTGGACCAGGCTCTGGAAAATTAGCAGTTGCATTAAGCGAACTTTATCATGATGCAATAAACAATATAGATTCTGGTTATGCAAAATTTGAAACATTTCCTGTTTGA", "aa": "MTAFNLKKYFKLQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQSTFRVNKLQNILSEHNINVVKHYKIDDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPV", "aa_hexdigest": "917f5ea40b0e64b26fd967b146037f0d", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0025EA5487", "query_cov": 0.9904306220095693, "subject_cov": 0.8922413793103449, "identity": 0.565, "score": 242.0, "evalue": 4.02e-78, "valid": false, "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_A0A348P2W6" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 167241, "stop": 168173, "observations": { "mutation": [ 167868 ], "stop": [ 167868 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7T6", "query_cov": 1.0, "subject_cov": 0.4247967479674797, "identity": 0.99, "score": 407.0, "evalue": 1.53e-139, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQSTFRVNKLQNILSEHNINVVKHYKIEDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPVWNLAVDHPLNLAYEAATTDLNDQNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINIIGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK" }, "paralog": false, "identity": 0.9870967741935484, "score": 609.0, "evalue": 2.92e-221, "description": "Internal stop codon at 167868. Nonsense mutation around 167868.", "psc": { "uniref90_id": "UniRef90_D1J7T6", "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_D1J7T6", "cog_id": "COG4868", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7T6", "UniRef:UniRef50_D1J7T6", "COG:COG4868", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T6" ], "product": "DUF1846 domain-containing protein" } }, "id": "AHCKFKLKGB_336", "locus": "GCA013367975_00309" }, { "type": "cds", "sequence": "contig_1", "start": 167892, "stop": 168719, "strand": "+", "frame": 3, "gene": null, "product": "DUF1846 domain-containing protein", "db_xrefs": [ "COG:COG4868", "COG:S", "SO:0001217", "UniRef:UniRef50_D1J7T6", "UniRef:UniRef90_D1J7T6" ], "nt": "TTGAATTTAGCTTATGAAGCTGCAACAACAGATTTAAATGATAAAAACAAAATCGATCCTTATTATTTAAAAAGTCGCAATAAAATAGCAACAAATTATAACCGCGATATTCAAGCATTTCCAATTTTGAGTCAAATAATGAACAAAATTCAAAAGCGTAATATTTATGATTCACCAACATCAATGGGAGTTAATATGGTTGGATTTTGTTTTAATAATGAAGATGAAATTAAACAAGCAGCAAATAAAGAAATAATTCGTCGTTACTATCAAAGTGCTGTTGCTTTTAAAAAAGGTATGGTTTCTAAATCAGTATTAATAAGAAATGCAAACTTGATATCTAAAAATAATATTAATTTTTCAATTAGACCTTGCATTGAAATTACTAACAAATTAGCAAACCAACTAAATATGAATATCACAACCTTTACCTTAAACAACAATATAAATATTACAGGTAAGACATCAGCGCTTCTTGGCTCATCATCAGCTGCGTTATTAAATGCATTAAAATATCTAGCAAATATTCCTAAAGAAATTCATTTGCTATCAAAAGAAGCAATTGAATTAGTTCAAAATCTTAAAGTAAATGTTTTTAAAAGCAAAAATCCTCGCTTACATATGAATGAAACTTTAATAATTTTAGCAATGTCTGCTAAGAGCAATCAATATGCAAAATTAGCCTATGATCAACTAAAAAATTTAGCAGGTATCGAAGCACATTGTAGCGCAATACTTTCTATGCAAGATATTGAAACATACAATCACTTAAATATTAACATTACTCAAGAACCAAAATACGATAATGACACCCTATATCATAAATAG", "aa": "MNLAYEAATTDLNDKNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNINFSIRPCIEITNKLANQLNMNITTFTLNNNINITGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK", "aa_hexdigest": "7067bf5c48e5e6334573949dcd9cba51", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 167592, "stop": 168719, "observations": { "mutation": [ 167868 ], "stop": [ 167868 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7T6", "query_cov": 1.0, "subject_cov": 0.5589430894308943, "identity": 0.982, "score": 521.0, "evalue": 5.08e-183, "gene_start": 1, "gene_end": 275, "reference_start": 218, "reference_end": 492, "reference_sequence": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQSTFRVNKLQNILSEHNINVVKHYKIEDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPVWNLAVDHPLNLAYEAATTDLNDQNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINIIGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK" }, "paralog": false, "identity": 0.984, "score": 729.0, "evalue": 9.42e-268, "description": "Internal stop codon at 167868. Nonsense mutation around 167868.", "psc": { "uniref90_id": "UniRef90_D1J7T6", "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_D1J7T6", "cog_id": "COG4868", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7T6", "UniRef:UniRef50_D1J7T6", "COG:COG4868", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T6" ], "product": "DUF1846 domain-containing protein" } }, "id": "AHCKFKLKGB_337", "locus": "GCA013367975_00310" }, { "type": "cds", "sequence": "contig_1", "start": 168773, "stop": 169336, "strand": "-", "frame": 3, "gene": "comEB", "product": "Deoxycytidylate deaminase", "db_xrefs": [ "COG:COG2131", "COG:FJ", "SO:0001217", "UniRef:UniRef50_B3PML8", "UniRef:UniRef90_D1J7T7" ], "nt": "ATGTTAAAATATTTTATTATGAATAATAAAACAAGAAAATTAATTTTTAACAACCATAAAGATAATTTAAAATGGGATATTTATTTTATGTCGCTTGCCAAATTAAGTGCATTACGTTCAAAAGATCCTTTAACTAAGGTGGGAGCTTGTATTGTAAGCCCAGATAATTATGTAATATCGCTAGGATATAATGGTATGCCAACAAGTTATTTAAATAAAGAAGTTAACAATGATGATTTATTCACTTGGAATAGACCTGTGACCCAAAACGATGTCTTAAATTCCAAATATACTTACGTTGTCCATGCCGAAGCAAATGCAATAATAAATGCAAATATTACAAATTCAAAGATTATACCAGGATCTATATTATACGTAACACATTCACCTTGCTACCACTGTGCTAAGCTAATTGTCCAATCTAAGATTTCTAAAGTTGTTTATGCAGTTGCATATAAGCCTGATTCAGATGATTTTAAGGCTTCAAATAAAATTTTTGCTGCATTTAATATCGAATGTAATGCAATAGAAAATGATTTTGATTTAGATTTTCATATAAAATAA", "aa": "MLKYFIMNNKTRKLIFNNHKDNLKWDIYFMSLAKLSALRSKDPLTKVGACIVSPDNYVISLGYNGMPTSYLNKEVNNDDLFTWNRPVTQNDVLNSKYTYVVHAEANAIINANITNSKIIPGSILYVTHSPCYHCAKLIVQSKISKVVYAVAYKPDSDDFKASNKIFAAFNIECNAIENDFDLDFHIK", "aa_hexdigest": "16f1b51dc2dc12b4dafafbf11e1af2a1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7T7", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9840000000000001, "score": 377.0, "evalue": 2.61e-132, "valid": true, "gene": "ComEB", "product": "Deoxycytidylate deaminase", "uniref50_id": "UniRef50_B3PML8", "cog_id": "COG2131", "cog_category": "FJ" }, "pscc": { "uniref50_id": "UniRef50_B3PML8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PML8" ], "product": "Deoxycytidylate deaminase" }, "genes": [ "comEB" ], "id": "AHCKFKLKGB_338", "locus": "GCA013367975_00311" }, { "type": "cds", "sequence": "contig_1", "start": 170049, "stop": 170363, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTGCATCAAATATAGGTTCTCTGTTTACTGCGTTGAATTCATATACGAATTCGCCTTTTACATATGCCGGGTCTATTTTACATGAAGCGGCTACTAATCCTGGTACTCCTACTGCTAGTAAGGACCCAAGACCAAGTAATCATAATTTTGATTTAGTTTTCATTTTTATCCTTAAAATTTTAAATTTTTATATATTGTTTTGCATATCTTTGTTGATATACAAATATCTTGTTTGTAAATATTTAAACAATAATTGTTTAAAGAATGTTATTGTAATTTATACCAACATTTTTAATTTTAAAGGTCATTAA", "aa": "MSASNIGSLFTALNSYTNSPFTYAGSILHEAATNPGTPTASKDPRPSNHNFDLVFIFILKILNFYILFCISLLIYKYLVCKYLNNNCLKNVIVIYTNIFNFKGH", "aa_hexdigest": "a53c820f46a798cbe97b8216bf9aa744", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11735.496999999987, "isoelectric_point": 9.074908256530762 }, "id": "AHCKFKLKGB_339", "locus": "GCA013367975_00312" }, { "type": "cds", "sequence": "contig_1", "start": 170496, "stop": 170669, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCATTGCCTTTTTCGTCTGTTGCGGTTTTAGTTGGTTGTGGATATTCAGATTCACTTGGAATTTCATCTGAATCAAATACATATTGTTTATTAGTTCCTTTTTCAGTTAAAACAATAGCTTCTGCTAATTCTAATCTATATTTAGCAAAAGTAGGTTTTACTAAATAG", "aa": "MYSLPFSSVAVLVGCGYSDSLGISSESNTYCLLVPFSVKTIASANSNLYLAKVGFTK", "aa_hexdigest": "ac474b8fda57dc9aa62d05958a050dda", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6001.851000000001, "isoelectric_point": 7.759852409362794 }, "id": "AHCKFKLKGB_340", "locus": "GCA013367975_00313" }, { "type": "cds", "sequence": "contig_1", "start": 171022, "stop": 171366, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCGGCATTGATGCTAATAAATTATTTGAAAAAGATAAATTTATTCAAAAAGTATCAGCTGAAGGCAATTTAAAAGACAGAGAAGCAATTACTTTCGAATCAATTACTGGAAAAGAAAATGATGCCAACATTTCAAGCTTCTTTGAATCTCAATTTGCATCAGGAACATATGACTTTATGCCAGCTCCATCTGACTACATTGATGCATTAAATGCTAAAAATGAACAAGTTGTTTTAAAATCAAATGGTAATGCTTTATCGAAATCTGAAACAGAAGCATATCGTAACGCTTTAAATTCAATGGATAAAGAAAGCTTACAATACAAAATTGGTCAATACTGA", "aa": "MFGIDANKLFEKDKFIQKVSAEGNLKDREAITFESITGKENDANISSFFESQFASGTYDFMPAPSDYIDALNAKNEQVVLKSNGNALSKSETEAYRNALNSMDKESLQYKIGQY", "aa_hexdigest": "61498998f44a5a027f596af9e671dc20", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12769.975299999986, "isoelectric_point": 4.715158271789551 }, "id": "AHCKFKLKGB_341", "locus": "GCA013367975_00314" }, { "type": "cds", "sequence": "contig_1", "start": 171661, "stop": 171891, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTAAATGAACGTTTGAATGTTCAAACTTCAATCTTTGGATTTGTTAGAACACCATTCATTTCTTCAAAATTAGGCAAAAATTACAAATTCAATGAAAACTTTGCTAAATTAGTATATGGATCTTCATTAGCAGATCTTGACAGTGGAAAAGCAGACACTTCAGAATCATATCTATTTGGTAAAGGTTTAAGCTTCAGAACACTTCTATATGCAGCAGTAAACTGA", "aa": "MKLNERLNVQTSIFGFVRTPFISSKLGKNYKFNENFAKLVYGSSLADLDSGKADTSESYLFGKGLSFRTLLYAAVN", "aa_hexdigest": "aeaa1115da145dd2d2daad202e0b9c27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8480.571899999995, "isoelectric_point": 9.572346305847166 }, "id": "AHCKFKLKGB_342", "locus": "GCA013367975_00315" }, { "type": "cds", "sequence": "contig_1", "start": 171994, "stop": 172308, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATACCATTGTAGACTTTAGAGACCAAGCTAACTCACTATTTGCAATTGACAAAGATGGAAACAAAGTTGATTTTGGAAAAGAATTAGGTAAGGAATTAAGTCCTTCTGAAAACGCTAAAGCTTCAGAAACTGAATCATCAATTTCTGAAAAGATGAAATCAGCAGGATTTAAAGTTCTTCAAACAAAAATGAAAGAATTACTAGATGAATTCTTTAAAGAACAAAACATTACCGATCCTGAAAAACAAACAATTGAATTTGAAGTAGGATTTAGATATTTAAATGCTCCACAACTATACATTAATGCATGA", "aa": "MNTIVDFRDQANSLFAIDKDGNKVDFGKELGKELSPSENAKASETESSISEKMKSAGFKVLQTKMKELLDEFFKEQNITDPEKQTIEFEVGFRYLNAPQLYINA", "aa_hexdigest": "43ec1ebef38cdd4c2b074938340a4aec", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11817.13879999999, "isoelectric_point": 4.776658058166503 }, "id": "AHCKFKLKGB_343", "locus": "GCA013367975_00316" }, { "type": "cds", "sequence": "contig_1", "start": 172513, "stop": 172851, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAGGTTGCGCACCAAACAGGTGACCAAAAAACTAAGTTCAAACAAGCCTTCCCAGAAATTGCTAAATTAGCTGATAAATTGTTAGAATATGCAAAAACAAACAAACCAAATTTATCATTGGATTTTGAAAAAATTGATCAAATTGACAATAGATATTTAAATTCAAACTTACCTGCAATTTTAGCAGCATATAAATTAGAACAAGATACCAATACTAAAAAATATGTAATTGCTAAAAAAACTGTTAAAGGTTCAAAACCAAAACCAATCGAATTCGATTTTGGAAAAGATAGTTCAGGAAAATCAATAGAACCAACCGATCTATATCAATGA", "aa": "MVKVAHQTGDQKTKFKQAFPEIAKLADKLLEYAKTNKPNLSLDFEKIDQIDNRYLNSNLPAILAAYKLEQDTNTKKYVIAKKTVKGSKPKPIEFDFGKDSSGKSIEPTDLYQ", "aa_hexdigest": "3fd1d0dc8a4bc44ea30f09f38abe420c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12694.398199999983, "isoelectric_point": 9.301514625549316 }, "id": "AHCKFKLKGB_344", "locus": "GCA013367975_00317" }, { "type": "cds", "sequence": "contig_1", "start": 173098, "stop": 173556, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAATATATATTACAAAGAATAGCGTTTGCTATTCTTACTCTATTTATTATCTCTATTTTCGTTTATGTGCTGGTTGCTGCATTTGGAAACAACCCAGTTAATGACTTAGCACAAGCACAATTTCAAGCAAGCAAAAAATCAGGATTAACATATACTGAAATATTGCATAAACTTGAAATTGAAAACGGATTAAGATATAAAGATAACCCGGACATTTTAGTTCCTATAATTGTTAGATATGGCAATTATATGAAAAACATTTTTACGAAGGGCGATTTTGGATTTTTATATAATCCAGCTAACAACCCAAACTCTGCTTTATATACAAATATGGCAAAACTATTCTTTACCCCCCTACAATATTCATTAATAATCACTGTTCCAACATTCTTTATTAGTTCAATATTGGGTATTGCAATAGGTGTCATTGCCGGATATAAGCGTGGAAAATGA", "aa": "MTKYILQRIAFAILTLFIISIFVYVLVAAFGNNPVNDLAQAQFQASKKSGLTYTEILHKLEIENGLRYKDNPDILVPIIVRYGNYMKNIFTKGDFGFLYNPANNPNSALYTNMAKLFFTPLQYSLIITVPTFFISSILGIAIGVIAGYKRGK", "aa_hexdigest": "6ca28f82d5a3d4f57dd1e00d12085b0f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17086.889199999983, "isoelectric_point": 9.63056125640869 }, "id": "AHCKFKLKGB_345", "locus": "GCA013367975_00318" }, { "type": "cds", "sequence": "contig_1", "start": 173614, "stop": 173952, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTCCTATTGTAATAACTATTTCAGTCGCATTAGGAATTTCTAGCGTTGTGCCTAGACCTGGTGAACAATCATTCGGAGTAATGTTTGTTTCTTATTTACCTCCTATATTTGTTATGTCAATAACCTCATTAGCAGGTTATGTAACATATACAAGAAATCAAGTTATTACAGTATTGACATCAAATTTCGTTTTAATAGCTAAAACAAAAGGATTGAGTTCTTCACAAATTTTCTTTAAATATGTTTTAAGAAATATTTCAATTCCTTTATTTAGCCTAGTTTTTGGGTCATTTATTGGTCTATTATCAGGATCAATAATTATTGAACAATATTGA", "aa": "MAPIVITISVALGISSVVPRPGEQSFGVMFVSYLPPIFVMSITSLAGYVTYTRNQVITVLTSNFVLIAKTKGLSSSQIFFKYVLRNISIPLFSLVFGSFIGLLSGSIIIEQY", "aa_hexdigest": "d6799819950cb17780ac61da17767541", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12173.337999999989, "isoelectric_point": 9.77445468902588 }, "id": "AHCKFKLKGB_346", "locus": "GCA013367975_00319" }, { "type": "cds", "sequence": "contig_1", "start": 174245, "stop": 174871, "strand": "+", "frame": 2, "gene": "dppC", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG1173", "COG:EP", "SO:0001217", "UniRef:UniRef50_D1J7U0", "UniRef:UniRef90_D1J7U0" ], "nt": "ATGAATATAAATGAAGTTAAAGCCTTTAACAAAGAATATAAAATTAATACTGAATTAGAGAAAAAATTTAAATTTGTTGCACCAGAAGATAGAATAGTAATCTCTTCTGTTGCAGGTAAGCCTAAGAAATTAGGTATAGAAATTTTAAAGAGATTTTTTACTAAACCAGTAGTATTAATAGCACTAGCAGTATTTATAATTATTTTACTGTTATCTATATTAGTTCCTGCTAATGGAATTTCTTACTATAAACCAAACGATCCAATTTCAGATAATGCATTTGTAAAGAATTTACCTCCTGCATCTTCTCCAATTGTTACTCAAGCATTAGAATCAAATGATCCCGTATTCAAATTTTATCAATTTGTCTTAAATAGGTCAGAAACCGATCCTGTATATAAGGCTAGAATGGCATTTTTCTTAAATGGCTTTCAAGCAAAAGTTAATGATGTTGGGATCTATGTTGCAAAATATAATGCTTATGATTTAGTAGTTGCTAATTACATAAATGGAATTATTTCTAAGAATCAAATAACAGATGCTGAATTCTCAAAAATATTTAATGAACAAAAAAACTTCTATTCTCATGTCTTATTAGGAACATCATCAATAGGCTACGATATTTGA", "aa": "MNINEVKAFNKEYKINTELEKKFKFVAPEDRIVISSVAGKPKKLGIEILKRFFTKPVVLIALAVFIIILLLSILVPANGISYYKPNDPISDNAFVKNLPPASSPIVTQALESNDPVFKFYQFVLNRSETDPVYKARMAFFLNGFQAKVNDVGIYVAKYNAYDLVVANYINGIISKNQITDAEFSKIFNEQKNFYSHVLLGTSSIGYDI", "aa_hexdigest": "9c73b7dc4e06fc7fd365e3da9f6bb7f6", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dppC" ], "truncated": "3-prime", "pseudogene": { "start": 174245, "stop": 175174, "observations": { "mutation": [ 174869, 174881, 174893, 175046, 175118 ], "stop": [ 174869, 174881, 174893, 175046, 175118 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U0", "query_cov": 1.0, "subject_cov": 0.49056603773584906, "identity": 0.986, "score": 392.0, "evalue": 1.87e-134, "gene_start": 1, "gene_end": 208, "reference_start": 1, "reference_end": 208, "reference_sequence": "MNINEVKAFNKEYKINTELEKKFKFVAPEDRIVISSVAGKPKKLGIEILKRFFTKPVVLIALAVFIIILLLSILVPANGISYYKPNDPISDNAFVKNLPPASSPIVTQALESNDPVFKFYQFVLNRSETDPVYKARMAFFLNGFQAKVNDIGIYVAKYNAYDLVVANYINGIISKNQITDAEYLKIFNEQKNFYSHVLLGTSSIGYDIWTTAWYATWRAIKIALIVVILEAIIGISIGAFLGFYAGKLIDTIIMRVIEIFLAPPTLIWILLFVSIIGANEWSLVLTLTIVGWPGFVGITRMFIITVKDEEFIIAAKAIGVGTPRRIFVHALPTIIGKIANNIVRSVPGVILWIASLAFLGFFKEKKDTNLGQMLIEASQEVGSNIWIIALPTIILLFLSLSLNFIALGVHDALDPRVMNKGKGK" }, "paralog": false, "identity": 0.8770226537216829, "score": 509.0, "evalue": 4.05e-183, "description": "Internal stop codon at 174869, 174881, 174893, 175046, 175118. Nonsense mutation around 174869, 174881, 174893, 175046, 175118.", "psc": { "uniref90_id": "UniRef90_D1J7U0", "gene": "DppC", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_D1J7U0", "cog_id": "COG1173", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U0", "UniRef:UniRef50_D1J7U0", "COG:COG1173", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U0" ], "product": "Oligopeptide transport system permease protein" } }, "id": "AHCKFKLKGB_347", "locus": "GCA013367975_00320" }, { "type": "cds", "sequence": "contig_1", "start": 175145, "stop": 175300, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCATTATTACAGTAAAAGATGAAGAATTCATCATTGCTGCAAAAGCAATTGGAGTTGGTACACCAAGAAGAATTTTCGTTCATGCATTACCAACTATAATTGGAAAGATTGCAAATAATATAGTAAGATCAGTACCAGGTGTTATTCTATGA", "aa": "MFIITVKDEEFIIAAKAIGVGTPRRIFVHALPTIIGKIANNIVRSVPGVIL", "aa_hexdigest": "766e71a14c489f6cc7701055b90c904a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5513.6307, "isoelectric_point": 10.266412925720214 }, "id": "AHCKFKLKGB_348", "locus": "GCA013367975_00321" }, { "type": "cds", "sequence": "contig_1", "start": 175361, "stop": 175519, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGATTGAAGCTTCACAAGAAGTTGGCTCAAATATTTGGATTATTGCTTTGCCTACAATTATTTTATTGTTTTTATCATTATCATTGAATTTCATTGCCTTAGGTGTGCACGATGCACTTGACCCACGTGTAATGAACAAAGGAAAAGGTAAGTAA", "aa": "MLIEASQEVGSNIWIIALPTIILLFLSLSLNFIALGVHDALDPRVMNKGKGK", "aa_hexdigest": "67d842025be02f62258fdb30e5d0caef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5661.721400000002, "isoelectric_point": 6.518659400939941 }, "id": "AHCKFKLKGB_349", "locus": "GCA013367975_00322" }, { "type": "cds", "sequence": "contig_1", "start": 175521, "stop": 175895, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGCAAAAATTATTAATAAACACGACAATTTAGAAAAAAACAACTCAGATACTATAAAACATCAAAAAATTAATAAAAGTACTAGAGTTTTAGATTCAATTCGTGTTGAACCTCTAAAAAATAATCAAATATTAAAAGTTAGAAATTTACATGTTAGTTTCCCTCAAGGTCATAAGAAAAGCATCCATATTGTTAGAGGTGTTGATTTAGATGTCCATAGAGGCGAAATAATTGGCCTCGTTGGAGAATCTGGTTCTGGTAAGTCAGTAACTTCAAAAACCTTAATTAATGTTAATGAATCAGGTATTATAAGTGCTGATCAAATTCAAATTGGCGAATATGAACTAACAAATATTAAAAAACAAAAATATTGA", "aa": "MAKIINKHDNLEKNNSDTIKHQKINKSTRVLDSIRVEPLKNNQILKVRNLHVSFPQGHKKSIHIVRGVDLDVHRGEIIGLVGESGSGKSVTSKTLINVNESGIISADQIQIGEYELTNIKKQKY", "aa_hexdigest": "181d13bbc0db6f4b43d159cf046d10dd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 56, "aa_cov": 0.43548387096774194, "hmm_cov": 1.0, "evalue": 1.234376942409523e-09, "score": 34.1786003112793, "start": 66, "stop": 119 } ], "seq_stats": { "molecular_weight": 13900.774199999989, "isoelectric_point": 9.734097480773926 }, "id": "AHCKFKLKGB_350", "locus": "GCA013367975_00323" }, { "type": "cds", "sequence": "contig_1", "start": 176073, "stop": 176408, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FD1CF63" ], "nt": "TTGCGTTCAGCTGAAAAAATATTTTATGCATACCCTCACACTCTATCGGGAGGTATGAAACAAAGAATTGTTATTACAATGGTTGTTGCTTTGAAGCCAGAATTAATAATTGCTGATGAACCAACAACAGCATTAGACCCAACAGTTCAAGCCAGCGTTTTGGCATTATTTGAAGATATTAGACAAAAAATGGGAATTTCAATAATTTTAATAAGTCACAATATATCTGTTATTGCAAAATTTTGTGATTATATTTATGTTATGTATGCTGGTAGAATTGTTGAAAAAGGAACCAAACAAGAAATCTTTACAAATCCTGCCCACCCATATACTTGA", "aa": "MRSAEKIFYAYPHTLSGGMKQRIVITMVVALKPELIIADEPTTALDPTVQASVLALFEDIRQKMGISIILISHNISVIAKFCDYIYVMYAGRIVEKGTKQEIFTNPAHPYT", "aa_hexdigest": "aa241ded370b8c4f868d7d0398dcf46a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FD1CF63", "query_cov": 0.9099099099099099, "subject_cov": 0.9439252336448598, "identity": 0.525, "score": 119.0, "evalue": 7.69e-33, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000FD1CF63" }, "pscc": { "uniref50_id": "UniRef50_UPI000FD1CF63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FD1CF63" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_351", "locus": "GCA013367975_00324" }, { "type": "cds", "sequence": "contig_1", "start": 176478, "stop": 176597, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATTTACCTCTTGGTGATCCATTTGTATACCGTAATGACTACGCATTAGAAATAGACTATTTAAAAGAACCTCCTCTAATTCCAATTACTGAAACCCATGCTGCCGCAACATGA", "aa": "MANLPLGDPFVYRNDYALEIDYLKEPPLIPITETHAAAT", "aa_hexdigest": "89750929c15eade9b04e273a06f3c46d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4373.931700000001, "isoelectric_point": 4.290286827087402 }, "id": "AHCKFKLKGB_352", "locus": "GCA013367975_00325" }, { "type": "cds", "sequence": "contig_1", "start": 176680, "stop": 177096, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "nt": "ATGAATAATAAAAATTTTAAAGATAAAAAAGTTATCTTATCGATAGATAATCTAAAAAAATACTTCGTTAATCAAGGAATGATAAATAAGGCTGTTGATGGAGTTTCTTTTGATGTACATGAAGGAGAAATAGTTGGTTTAATTGGAGAATCAGGTTCAGGTAAAACAACCGTTGGTTCAACAATACTTAGACTATATGATGATTACAACGGATTTGTAAGATTAGAAGACAAAATAATTTCAGGTAAGCGTATTAGTGAATCTTTAAATCGTTTTTTAAGAAAAAATGTGCAAATGATTTTCCAAGATCCACATGCTTCATTAAATAGTCAACAAAATATTTATTCAATTTTAAAAGAACCTTTATTAGTTAATGGAGTAATGAAGGACAAAATTAAGGATATTTTTAAAGATTGA", "aa": "MNNKNFKDKKVILSIDNLKKYFVNQGMINKAVDGVSFDVHEGEIVGLIGESGSGKTTVGSTILRLYDDYNGFVRLEDKIISGKRISESLNRFLRKNVQMIFQDPHASLNSQQNIYSILKEPLLVNGVMKDKIKDIFKD", "aa_hexdigest": "91715e0a19db33f76a755f8881eb8d8a", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00249F2F44", "query_cov": 0.9202898550724637, "subject_cov": 0.9921875, "identity": 0.701, "score": 184.0, "evalue": 1.13e-57, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000CA0BF5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000CA0BF5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_353", "locus": "GCA013367975_00326" }, { "type": "cds", "sequence": "contig_1", "start": 177325, "stop": 178494, "strand": "+", "frame": 1, "gene": "dppD", "product": "Oligopeptide transport ATP-binding proteinhomolog", "db_xrefs": [ "COG:COG0444", "COG:EP", "SO:0001217", "UniRef:UniRef50_B3PML2", "UniRef:UniRef90_D1J7U2" ], "nt": "ATGTATGCAAATGTTGATAGGTTAATTGAATTTTATTATGAAACACAACGTAAATATCGTAATAAAGAATTACCTGGCCCACAAATTGCTTTAAATGAAAAAGAAGAAAAATTAAAATACATTAAATGTTTAAGCAAGACTTCAAAGATTGCTTATGAAGCTTCACTTGAATTAAAAGAAGTTAAAAAACAAATATTTAATTTAAGACATAAAATTGAAAAAATATCTTCTGAAAGCAAGAATACATTTGCAAACTATATTCATGAATCAAAAAATGAAAAGAGTTTAATTGACATAGCAAGATTAATGTCATGTGATTTAGACTTTTATTCATATAATTTAAAAAGTGAATTGTTGATGAATAAAAGGGGTCAAGTATTAGCAAAAATAAAACCATTTAATAAATTCCTAGGATTTAATAATATAAAGAAGTTAATTAATGAACTAGATGAATATATTAATGAATTTTATAAAACAAAATTAGCCCCATTGCCATATTCAAAAACATTGAAATCTGATATTAAAAATGTTTTAGAAAAAGACTTTGATTTTAAATTCGATGAATATACTAAAATATCAGAATTAGAAAAACAAAAGTTAGATTCAGAATTAAAACAATTACTATCAAGAGAAGTTGAAATTAAAAAAGTAATAAAAGAAAATCCAAATCCTGAAATTACTTCAGAACAACTTCAAGAAGCAAAAAACGATCTAGAATTAGGAAAACAAGCATATCTTGAAGGCAGATCTAAATATTTAATTTCATATAAAGCCGAACTTGAAAGCTTGTATAAAAATCTTGAAGAACAAAAGAAATTCTATCTTGAATTGCGCAAGCAACAAGATTATTGCAATAACAAATATAAAGAATTGAAACAAAAATTCTTTGAATATGTTGAACAATTACGTCAAAAAGAAAAAGATAAGATTTTATTAAAAATAGCTGAATTAAAACAAAATCAAGACAAATCAAACAAAGATAAAAAGTCATTAGCAAAATTACAAAGCAAATTAAAATCAATTAAGGTTCAATACAATACAATATTAAAAATGTACCATTCAGATATTTCATTAAAAGAAGATACATTGAAATCATTTGATATTGAAAGAAAATATCTTGATAAAGATATAAATAATATTTATGTTTTATTAGGAATTGACCACAAGTGA", "aa": "MYANVDRLIEFYYETQRKYRNKELPGPQIALNEKEEKLKYIKCLSKTSKIAYEASLELKEVKKQIFNLRHKIEKISSESKNTFANYIHESKNEKSLIDIARLMSCDLDFYSYNLKSELLMNKRGQVLAKIKPFNKFLGFNNIKKLINELDEYINEFYKTKLAPLPYSKTLKSDIKNVLEKDFDFKFDEYTKISELEKQKLDSELKQLLSREVEIKKVIKENPNPEITSEQLQEAKNDLELGKQAYLEGRSKYLISYKAELESLYKNLEEQKKFYLELRKQQDYCNNKYKELKQKFFEYVEQLRQKEKDKILLKIAELKQNQDKSNKDKKSLAKLQSKLKSIKVQYNTILKMYHSDISLKEDTLKSFDIERKYLDKDINNIYVLLGIDHK", "aa_hexdigest": "15fb21b6471bb85275d4b7699dc7f1cd", "start_type": "ATG", "rbs_motif": null, "genes": [ "dppD" ], "truncated": "both", "pseudogene": { "start": 177025, "stop": 178797, "observations": { "mutation": [ 177094, 177205, 178492, 178561 ], "stop": [ 177094, 177205, 178492, 178561 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U2", "query_cov": 1.012853470437018, "subject_cov": 0.4651711924439197, "identity": 0.98, "score": 711.0, "evalue": 1.19e-250, "gene_start": 1, "gene_end": 389, "reference_start": 216, "reference_end": 609, "reference_sequence": "MNNKNFKDKKVILSIDNLKKYFVNQGMINKAVDGVSFDVHEGEIVGLIGESGSGKTTVGSTILRLYDDYNGFVRLEDKIISGKRISESLNRFLRKNVQMIFQDPHASLNSQQNIYSILKEPLLVNGVMKDKIKDIFKDWLNVKKNFKYTFQIEAMRLELQNYLEINKLAEPLFKKWTENLNKLEFNFDISREDNFSLFFGYLEEKQNVESIIINNMYANVDRLIEFYYETQRKYRNKELPGPQIALNEKEEKLKYIQCLSKTSKIAYEASLELKEVKKQIFNLRHKIEKISSESKNTFANYIHESKNEKSLIDIARLMSCDLDFYSYNLKSELLMNKRGQVLAKIKPFNKFLGFNNIKKLINELDEYINEFYKTKLAPLPYSKTLKSDIKNVLEKDFDFKFNEYTKISELEKQKLDSELKQLLSREVEIKKVIKENPNPEITSEQLQEAKNDLELGKQAYLEGRSKYLISYKAELESLYKNLEEQKKFYLELRKQQDYCNNKYKELKQKFFEYVEQLRQKEKDKILLKIAELKQNQDKSNKANNINLKKSLAKLQSKLKSIKVQYNTILKMYHSDISLKEDTLKSFDIERKYLDKDINNIYVLLGIDHKWVETNLKTADAMAGVDAKHMKWVWRKRYFDSKVSYPIAKLLISSILYKTIIYKALEDVGLLKQFAYRYPHEFSGGQLQRIVIARALIVEPQVIVADEPIASLDISIQAQVVNLLKELCIKKNIGLIFIAHDLSMIEYVADEVQIMHLGKIVESGKTEAIYANPIHPYTINLFKAIPKISNANEKFQNVSFALDYLDEQKFPNVPETFKVGEDHYVYGTASQLKQWTHNAKLEKEHKEL" }, "paralog": false, "identity": 0.9798319327731092, "score": 1127.0, "evalue": 0.0, "description": "Internal stop codon at 177094, 177205, 178492, 178561. Nonsense mutation around 177094, 177205, 178492, 178561.", "psc": { "uniref90_id": "UniRef90_D1J7U2", "gene": "DppD", "product": "Oligopeptide transport ATP-binding proteinhomolog", "uniref50_id": "UniRef50_B3PML2", "cog_id": "COG0444", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U2", "UniRef:UniRef50_B3PML2", "COG:COG0444", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_B3PML2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PML2" ], "product": "Oligopeptide ABC transporter ATP-binding protein" } }, "id": "AHCKFKLKGB_354", "locus": "GCA013367975_00327" }, { "type": "cds", "sequence": "contig_1", "start": 178627, "stop": 179166, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "nt": "TTGTATAAAACAATAATTTATAAAGCATTGGAAGACGTTGGATTATTAAAACAATTTGCTTATCGTTATCCTCATGAATTTAGTGGGGGTCAATTGCAAAGAATTGTTATTGCAAGAGCATTGATAGTTGAACCTCAAGTTATTGTGGCCGATGAACCTATTGCCTCACTAGATATATCAATTCAAGCACAAGTTGTTAACTTGCTAAAAGAATTATGTATTAAAAAGAACATCGGTCTTATATTCATAGCACACGACCTTTCAATGATTGAATATGTTGCTGATGAAGTTCAAATAATGCACCTTGGAAAAATTGTTGAAAGTGGAAAAACCGAAGCAATATATGCAAATCCAATTCACCCATATACAATAAACTTATTTAAAGCTATTCCTAAAATTTCTAATGCTAATGAAAAATTCCAAAATGTTTCATTTGCTTTAGATTACTTAGATGAACAAAAATTCCCTAATGTTCCTGAAACATTTAAAGTTGGCGAAGATCACTATGTTTATGGAACTGCTAGTCAACTTAAACAATGA", "aa": "MYKTIIYKALEDVGLLKQFAYRYPHEFSGGQLQRIVIARALIVEPQVIVADEPIASLDISIQAQVVNLLKELCIKKNIGLIFIAHDLSMIEYVADEVQIMHLGKIVESGKTEAIYANPIHPYTINLFKAIPKISNANEKFQNVSFALDYLDEQKFPNVPETFKVGEDHYVYGTASQLKQ", "aa_hexdigest": "f1efd8141ea362f19edc8aad725ebec4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024CC3F60", "query_cov": 1.0, "subject_cov": 0.8647342995169082, "identity": 0.737, "score": 267.0, "evalue": 7.16e-89, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A4R0XTG3" }, "pscc": { "uniref50_id": "UniRef50_A0A4R0XTG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_355", "locus": "GCA013367975_00328" }, { "type": "cds", "sequence": "contig_1", "start": 179276, "stop": 179998, "strand": "-", "frame": 3, "gene": "rsuA", "product": "Pseudouridine synthase", "db_xrefs": [ "COG:COG1187", "COG:J", "RefSeq:WP_047207614.1", "SO:0001217", "UniParc:UPI000640100A", "UniRef:UniRef100_UPI000640100A", "UniRef:UniRef50_D1J7U3", "UniRef:UniRef90_D1J7U3" ], "nt": "ATGGAAGAAAAAGCAAAACGAATTCAAAAAATTTTAAGTGAGCAAGGCATTTGTTCTAGAAGAAAAGCAGAAGAATTAATTTTATCGGGGAAGATTTTAATAAATAATAAAATTGCAAAATTAGGCGAATGTGTAACTATAAATGATGAAATTAAAATAAACAATAGAATTATCAAATTAAATAATAATGTTGCAAATAATTTTAAATACATTGTTTTAAATAAACCTATGAATACAATTTGTACAGCAAGTGATCCTCAAAATAGAAAAACAATTTACGAATATCTCAAAGTAAAAGAACGCTTATTTTCAATAGGAAGATTAGACTTTAATACAACAGGAATTATATTAATCACAAACGATGGAGAATTTGCTAATTTATTAAGCCATCCTTCTTCTGAAATTCCTAGAATTTATATTGCTACATTAGAACGTGAAATGCTTGATGGTGAATTAAACTTATTAAATTCAAATCACGTATTATTAAATGGCAAAATTTCAAAACAAAAAGTTACAAAATTAAGTGCTTTTAAATATGAAGTTCTACTATATGAAGGCAGAAACCATCACGTTAAAAATTTATTTTTGTTAGTAAATAATTATGTTAAAAAATTACATAGAAAATCTTTTGGATGCATTGATGATAGTAATTTAAAAATAGGTCAATATAGAGATTTAACTTCAAGCGAAATTAATAAATTATTAGCTTATATAAAGAAATAA", "aa": "MEEKAKRIQKILSEQGICSRRKAEELILSGKILINNKIAKLGECVTINDEIKINNRIIKLNNNVANNFKYIVLNKPMNTICTASDPQNRKTIYEYLKVKERLFSIGRLDFNTTGIILITNDGEFANLLSHPSSEIPRIYIATLEREMLDGELNLLNSNHVLLNGKISKQKVTKLSAFKYEVLLYEGRNHHVKNLFLLVNNYVKKLHRKSFGCIDDSNLKIGQYRDLTSSEINKLLAYIKK", "aa_hexdigest": "7b992beb87b2b0f94a0c088484ebfff0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000640100A", "ncbi_nrp_id": "WP_047207614.1", "uniref100_id": "UniRef100_UPI000640100A", "db_xrefs": [ "SO:0001217", "UniParc:UPI000640100A", "RefSeq:WP_047207614.1", "UniRef:UniRef100_UPI000640100A" ] }, "ips": { "uniref100_id": "UniRef100_UPI000640100A", "uniref90_id": "UniRef90_D1J7U3" }, "psc": { "uniref90_id": "UniRef90_D1J7U3", "gene": "RsuA", "product": "Pseudouridine synthase", "uniref50_id": "UniRef50_D1J7U3", "cog_id": "COG1187", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J7U3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U3" ], "product": "Pseudouridine synthase" }, "genes": [ "rsuA" ], "id": "AHCKFKLKGB_356", "locus": "GCA013367975_00329" }, { "type": "cds", "sequence": "contig_1", "start": 180106, "stop": 180360, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U4", "UniRef:UniRef90_D1J7U4" ], "nt": "ATGGAATATTTACAAAATATAGACTTAAGCAAAATAAGATCGCTTTGTTTACAAAAAAAGCGGGAATTTATCTTTAATTATTGCCTTTTTGAGCAGTTAAAAGATAAACAATTAAAACAAAAAAAATCACAAACAATCAATGACGAAGAAGATGATGATATTTCAAAATTAGAAAAATTTATTAAATTATTAAATTCTGATAATCAAAAAATCTTTTTTAATAATTTTATTTATAACAATCCTGAATCTGAATGA", "aa": "MEYLQNIDLSKIRSLCLQKKREFIFNYCLFEQLKDKQLKQKKSQTINDEEDDDISKLEKFIKLLNSDNQKIFFNNFIYNNPESE", "aa_hexdigest": "cc38cd88563039e12c5649d4cb46963b", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 180106, "stop": 180444, "observations": { "mutation": [ 180358, 180373 ], "stop": [ 180358, 180373 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U4", "query_cov": 1.0, "subject_cov": 0.75, "identity": 1.0, "score": 162.0, "evalue": 1.84e-50, "gene_start": 1, "gene_end": 84, "reference_start": 1, "reference_end": 84, "reference_sequence": "MEYLQNIDLSKIRSLCLQKKREFIFNYCLFEQLKDKQLKQKKSQTINDEEDDDISKLEKFIKLLNSDNQKIFFNNFIYNNPESEWYLAYWSKNAYYKKLNYIVNLFIDFINQ" }, "paralog": false, "identity": 0.9821428571428571, "score": 221.0, "evalue": 3.27e-76, "description": "Internal stop codon at 180358, 180373. Nonsense mutation around 180358, 180373.", "psc": { "uniref90_id": "UniRef90_D1J7U4", "uniref50_id": "UniRef50_D1J7U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U4", "UniRef:UniRef50_D1J7U4" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10245.543599999992, "isoelectric_point": 5.506072807312011 }, "id": "AHCKFKLKGB_357", "locus": "GCA013367975_00330" }, { "type": "cds", "sequence": "contig_1", "start": 180684, "stop": 180926, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACTCCAAAAAGTCTTTGATTCAGAAAAACATGAATATACATATACTCTTATTTCTGAATTTAACGAAGCAACAAAAACTAAAAAAGTTCAGGATATAAAAAATATTAATTTTGAGATTTTTTATAAGGTAAAAGGTTTATTGTATCAAGTAGAAAGATATACATATGAAATATATAGTAATAACAATTCTAATAAAGAATTAGTTTCAAATAAAAATGGCGTTGAATATGATTTATGA", "aa": "MKLQKVFDSEKHEYTYTLISEFNEATKTKKVQDIKNINFEIFYKVKGLLYQVERYTYEIYSNNNSNKELVSNKNGVEYDL", "aa_hexdigest": "68d68bc3c1ee038b0c6b99624d1449af", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9630.717699999997, "isoelectric_point": 6.574191093444825 }, "id": "AHCKFKLKGB_358", "locus": "GCA013367975_00331" }, { "type": "cds", "sequence": "contig_1", "start": 180942, "stop": 181508, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9K8", "UniRef:UniRef90_A0A454C9K8" ], "nt": "ATGTCATTGCCAGATAGAGATATTCCAACAGATATGAAACATTATTCATTTGTCGAACAAGTTAAATACAAATTTATACCAACTACACTGATGTTGGGAAAACATAATGAAAAATTATTTGATGTTGAAATATCAAAAAAAGAAATTTCTAACAAAAGTGGCATAACTAGTGATTTTTCTCAATTAATTCAAAGTGATATTAACGGTTTTAATGTATCTATTGATATTGATAAACAATTTATAAAGGGCAACTTGAATGCAAAAATTAATGATTTCGACAAAAATGAGCATAAGCAAATTTCAATAGATTCTTATTCATATTACGACAAAGTTTCTAAGGCAATAATAGCAAATAATGATAGTATTGGAGCAACAAGAGGCTTGCTTTTGCCTCTTAATTATTCAGGAAATTATTCATATTTACTAAATGTTAATTTTGGCGAAGATTTGAAAGGCTTTAAAATTAGATACAATCAAGAAATTTCAAAACCATTTTTTAATGAAAAAAGTGGCTTAATAAAATTAAGTGTAAACAATATATTTAAACAACAAAATTTTACAAAATGA", "aa": "MSLPDRDIPTDMKHYSFVEQVKYKFIPTTLMLGKHNEKLFDVEISKKEISNKSGITSDFSQLIQSDINGFNVSIDIDKQFIKGNLNAKINDFDKNEHKQISIDSYSYYDKVSKAIIANNDSIGATRGLLLPLNYSGNYSYLLNVNFGEDLKGFKIRYNQEISKPFFNEKSGLIKLSVNNIFKQQNFTK", "aa_hexdigest": "4fc0220be91be2dba96ae8e952b21794", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 180642, "stop": 181589, "observations": { "mutation": [ 180924, 181506 ], "stop": [ 180924, 181506 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9K8", "query_cov": 1.0, "subject_cov": 0.49343832020997375, "identity": 0.9259999999999999, "score": 345.0, "evalue": 4.73e-117, "gene_start": 1, "gene_end": 188, "reference_start": 167, "reference_end": 354, "reference_sequence": "MFPIVMQAINDQEPTKWIENINAYTTKQLYLDKWTNSFQKVDFKLQRLIENNKFIIEFKYLNYDQEIDDIDVKCSINNQLLKLQKVFDSDKHEYTYTLISEFNEATKTKKVQDIKNINFEIFYKVKGLLYQVERYTYEIYSNNNSNRELVSNKNGVEYDLWTKLRILSLADRDIPTDMEHYSFVEQVKYKFMPTTLVLGKHNKKLFDVEISKKEISNKSGIISDFSQLIQSDINGFNVSIDIDKQFIKGNLNAKINDFSKNEHKQISIDSYSYYDKVSKAIIANNDSIGAAKGLLLPLNYSGNYSYLLNINFGEDLKGFKIRYNQEISKPFFNEKSGLVKLNVNNIFKQQNFIKWSVVKAEDIRSLINNSNTLEELMKGGK" }, "paralog": false, "identity": 0.9396825396825397, "score": 592.0, "evalue": 1.14e-216, "description": "Internal stop codon at 180924, 181506. Nonsense mutation around 180924, 181506.", "psc": { "uniref90_id": "UniRef90_A0A454C9K8", "uniref50_id": "UniRef50_A0A454C9K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9K8", "UniRef:UniRef50_A0A454C9K8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9K8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 21586.231400000004, "isoelectric_point": 8.842499732971191 }, "id": "AHCKFKLKGB_359", "locus": "GCA013367975_00332" }, { "type": "cds", "sequence": "contig_1", "start": 181589, "stop": 181933, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U6", "UniRef:UniRef90_D1J7U6" ], "nt": "ATGTTAATTGCAAAAAAGAAAAAAATAATTTTTGGTTTATCTCTTGGATTAGCTTGCGCCGTTGGGGCGGGGGCATTAGTTGGAGTTTATTTTTTAGCATCTTCTAATTCAAATAAAAAAGCTAATAATCTAGACAATAAAAAGGTTAACAAAAAAGATTCAACAAATAACATTAATGACAACAAAAACATTAATTCTGGCAATGAAAACGAAAAAGATAACATTATTGATAACCAAAACGAAGATAGTAAAATTGAAGATTCTAATGAAGATGGCCAAGCGAATGATGACGATAAAAAGCTTAAACCAGAAACAAAGCCAATTATAAAAACAGAGATTAATTGA", "aa": "MLIAKKKKIIFGLSLGLACAVGAGALVGVYFLASSNSNKKANNLDNKKVNKKDSTNNINDNKNINSGNENEKDNIIDNQNEDSKIEDSNEDGQANDDDKKLKPETKPIIKTEIN", "aa_hexdigest": "5ec2f5272160ae6b950482f82b7becb2", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 181589, "stop": 182188, "observations": { "mutation": [ 181931, 182129 ], "stop": [ 181931, 182129 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U6", "query_cov": 1.0175438596491229, "subject_cov": 0.5771144278606966, "identity": 0.871, "score": 121.0, "evalue": 2.19e-32, "gene_start": 1, "gene_end": 114, "reference_start": 1, "reference_end": 116, "reference_sequence": "MLIAKKKKIIFGLSLGLACAVGAGALVGVYFLASSNSNKKANNLDNKKVNKKDSTNNNSDNKNINSGNENEKDNTIDNQNEDSKIEDSNEDGQANDDDKKLKPEVKPRPNIKTEINWREIFPDVKTSDYYNQLNFKDGQGFIDKEMITYIIKDILNRMTILDGDVSYAYKQEDDQNLYIAFKWENKSQKSNITYKFSVNRI" }, "paralog": false, "identity": 0.9104477611940298, "score": 298.0, "evalue": 1.2e-104, "description": "Internal stop codon at 181931, 182129. Nonsense mutation around 181931, 182129.", "psc": { "uniref90_id": "UniRef90_D1J7U6", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J7U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U6", "UniRef:UniRef50_D1J7U6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U6" ], "product": "Lipoprotein" } }, "id": "AHCKFKLKGB_360", "locus": "GCA013367975_00333" }, { "type": "cds", "sequence": "contig_1", "start": 182018, "stop": 182131, "strand": "+", "frame": 2, "gene": null, "product": "MHO_1590 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A6F3B0", "UniRef:UniRef90_UPI0035A6F3B0" ], "nt": "ATGATTACTTATATAATAAAAGATATTTTAAATAGAATGACTATTTTAGATGGCAATGTTTCTTATGCTTATAAACAAGAGGATGATCAAAATTTATATATTGCTTTTAAATGA", "aa": "MITYIIKDILNRMTILDGNVSYAYKQEDDQNLYIAFK", "aa_hexdigest": "fbfa7e545dfa804fbe96fcddc547dae2", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 181940, "stop": 182188, "observations": { "mutation": [ 182129 ], "stop": [ 182129 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0035A6F3B0", "query_cov": 1.0, "subject_cov": 0.45121951219512196, "identity": 1.0, "score": 77.8, "evalue": 5.11e-18, "gene_start": 1, "gene_end": 37, "reference_start": 27, "reference_end": 63, "reference_sequence": "MFPDVKTSDYYNQLNFKDGQGFIDKEMITYIIKDILNRMTILDGNVSYAYKQEDDQNLYIAFKWENKSQKSNITYKFSVNRI" }, "paralog": false, "identity": 0.975609756097561, "score": 164.0, "evalue": 1.43e-54, "description": "Internal stop codon at 182129. Nonsense mutation around 182129.", "psc": { "uniref90_id": "UniRef90_UPI0035A6F3B0", "product": "MHO_1590 family protein", "uniref50_id": "UniRef50_UPI0035A6F3B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0035A6F3B0", "UniRef:UniRef50_UPI0035A6F3B0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0035A6F3B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A6F3B0" ], "product": "MHO_1590 family protein" } }, "id": "AHCKFKLKGB_361", "locus": "GCA013367975_00334" }, { "type": "cds", "sequence": "contig_1", "start": 182197, "stop": 182421, "strand": "+", "frame": 1, "gene": "acpP", "product": "Acyl carrier protein", "db_xrefs": [ "COG:COG0236", "COG:I", "RefSeq:WP_020002664.1", "SO:0001217", "UniParc:UPI0002DDE617", "UniRef:UniRef100_A0A2K9YT00", "UniRef:UniRef50_A0AA43R1D2", "UniRef:UniRef90_A0A2K9YT00" ], "nt": "ATGGAATCTGAAAAAATTATAATTAATGCTATTAAAAAACTAACTAAAACAAAGTTTAATAAAGAAACCTTTATTCGCGATTTGAATATCGATAGTTTAGATTTGGTTATACTTGTTAGCGATTTAGAAGAGGCTTACAATATAAAAATAAGTGACGAAGAATTGCTTTCTTTAAAGACCATAAATGATATTATTGAATTATTTGATAAAAAAACGAATAAATAA", "aa": "MESEKIIINAIKKLTKTKFNKETFIRDLNIDSLDLVILVSDLEEAYNIKISDEELLSLKTINDIIELFDKKTNK", "aa_hexdigest": "0fda9036b020adeab1537197b28e01cc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002DDE617", "ncbi_nrp_id": "WP_020002664.1", "uniref100_id": "UniRef100_A0A2K9YT00", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002DDE617", "RefSeq:WP_020002664.1", "UniRef:UniRef100_A0A2K9YT00" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YT00", "uniref90_id": "UniRef90_A0A2K9YT00" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YT00", "gene": "AcpP", "product": "Acyl carrier protein", "uniref50_id": "UniRef50_A0AA43R1D2", "cog_id": "COG0236", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_A0AA43R1D2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA43R1D2" ], "product": "Acyl carrier protein" }, "genes": [ "acpP" ], "id": "AHCKFKLKGB_362", "locus": "GCA013367975_00335" }, { "type": "tRNA", "sequence": "contig_1", "start": 182454, "stop": 182529, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 66.8, "nt": "ATGGGTATAGTTCAATCGGCAGAACAACGGGCTTCAACCCCGTGTGTTGTGAGTTCAAGTCTTACTACCCATGCCA", "db_xrefs": [], "id": "AHCKFKLKGB_15", "locus": "GCA013367975_00336" }, { "type": "tRNA", "sequence": "contig_1", "start": 182636, "stop": 182710, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 76.0, "nt": "GCCGTCATAGCTCAGGGGCAGAGCACTTCCATGGTAAGGAAGGGGTCGTTGGTTCAATTCCAATTGACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 182676, 182678 ], "id": "AHCKFKLKGB_32", "locus": "GCA013367975_00337" }, { "type": "cds", "sequence": "contig_1", "start": 182820, "stop": 183899, "strand": "+", "frame": 3, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0006415", "GO:0016149", "KEGG:K02835", "RefSeq:WP_020002663.1", "SO:0001217", "UniParc:UPI0003822901", "UniRef:UniRef100_A0A2K9YT01", "UniRef:UniRef50_A5IZH6", "UniRef:UniRef90_D1J7U7" ], "nt": "ATGGAAAAGACAATGTACGATTCACTACTTTCAATTAAAAATCAATACGATGAATTAACTGAAAAATTAAACGACCCTAATATTTATAATGATATAAAAGCATATACAAGAATAGCAAAAGAAAAATCTGAGTTGCAAGAAATTTATGATGCTTTTACAAAATATCAAAACATTGAAGAACTATATTTCTTGTCAAAAGAAATTTTGGCAAATGAAAAAGATCAAGAATTAATTGAGCTTGCAAAAAACGACATTGACAATCAAATGAATCAATTGCAAGAAATAGAAGAAGAAATTAAAGAACTTTTATTGCCACAAGATGAAAATGACAAACGTGATGTTATTATGGAAATAAGAGGTGCAGCTGGTGGTGATGAAGCAAATATCTTTTCTGGTGATTTATTTAAAATGTATCAAAAATATGCAGAAATCAAAGGTTTTAAAATTAAAATAATTGATTCTACATATGGCGCTGCCGGAGGATATAGCCAAATTGTTTTTAGTATAAAAGGCGAAAAGGTTTTTTCACAACTAAAATTTGAACGTGGTGTACATAGAGTTCAACGTGTTCCTGCAACTGAAACTCAAGGACGTGTTCATACATCAACTGCAACTGTAACTGTATTGCCTGAAGTTGATGAAGATGTAAAAATTGAAATAAGACCAGAAGATATAGAAGTTAATGTATTTAGAAGTTCTGGAGCTGGCGGACAATCTGTTAATACAACGGACTCTGCAGTAAGAATTACTCATAAGCCTACAGGATTGGTTGTAACATCACAAGATGAAAGAAGCCAAATTCAAAATAAAGAAACAGCTTTAAAAGTTTTGAAAAGTAGATTGTACGAACTAGAATTAAGAAAACGTGAAGAAGCAGAAAGCGGACTTAGAAAATTAGCTGGAACAGGGGATAGATCAGAAAAAATTAGAACATATAACTACCCTCAAGATAGAATTACAGATCATAGAATTGGATTTTCAACTTCATTAAAAGTAGTTATGGAAGGCGAATTAGACAAAATTATTGAAGCATTAATAGCTGATGAAAAGGCTAAAAAAATCATTGAAGCAGGAAATTAA", "aa": "MEKTMYDSLLSIKNQYDELTEKLNDPNIYNDIKAYTRIAKEKSELQEIYDAFTKYQNIEELYFLSKEILANEKDQELIELAKNDIDNQMNQLQEIEEEIKELLLPQDENDKRDVIMEIRGAAGGDEANIFSGDLFKMYQKYAEIKGFKIKIIDSTYGAAGGYSQIVFSIKGEKVFSQLKFERGVHRVQRVPATETQGRVHTSTATVTVLPEVDEDVKIEIRPEDIEVNVFRSSGAGGQSVNTTDSAVRITHKPTGLVVTSQDERSQIQNKETALKVLKSRLYELELRKREEAESGLRKLAGTGDRSEKIRTYNYPQDRITDHRIGFSTSLKVVMEGELDKIIEALIADEKAKKIIEAGN", "aa_hexdigest": "c3c8ccaa135d7fe80bd1b50f2783a8ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0003822901", "ncbi_nrp_id": "WP_020002663.1", "uniref100_id": "UniRef100_A0A2K9YT01", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003822901", "RefSeq:WP_020002663.1", "UniRef:UniRef100_A0A2K9YT01" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YT01", "uniref90_id": "UniRef90_D1J7U7" }, "psc": { "uniref90_id": "UniRef90_D1J7U7", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A5IZH6", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_A5IZH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IZH6" ], "product": "Peptide chain release factor 1" }, "genes": [ "prfA" ], "id": "AHCKFKLKGB_363", "locus": "GCA013367975_00338" }, { "type": "cds", "sequence": "contig_1", "start": 183899, "stop": 184291, "strand": "+", "frame": 2, "gene": null, "product": "methyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022080B54" ], "nt": "ATGATAGATAAAGAAATTTTGCTACGTGAAAAAGAAAGATATAATTTACCCCTATATATTTCAAAAAAAGAATTAAGAATGCTAAAAAAAGATGTTCCTGTGCAAAAAATAATTGGATACCAGGAATTTCAAAATGTAATTTTAAATTTAAAATATAAAACCTTGATTCCTAGATATGAAACAGAAGAGGTTATTTTAGCTGCATATCCATTTATAAAAAAAGAATCTAAAGTATTGGATTTGGGGTGCGGTAGTGGCCTAATAGGACTTGCAATTAAAAAAAATAAAGATGCTAATGTTACATTAAGTGATATTAGCCGTTTTGCAATCAAACAAACAAAAATAAATGCCAAATTGAATAATTTAAATGTAAATGTAATTCGTTCAAATTGA", "aa": "MIDKEILLREKERYNLPLYISKKELRMLKKDVPVQKIIGYQEFQNVILNLKYKTLIPRYETEEVILAAYPFIKKESKVLDLGCGSGLIGLAIKKNKDANVTLSDISRFAIKQTKINAKLNNLNVNVIRSN", "aa_hexdigest": "fb92bb821032a6ab69fada4c0e251a7f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0022080B54", "query_cov": 0.8846153846153846, "subject_cov": 0.9274193548387096, "identity": 0.513, "score": 94.4, "evalue": 1.82e-22, "valid": false, "product": "methyltransferase domain-containing protein", "uniref50_id": "UniRef50_UPI0022080B54" }, "pscc": { "uniref50_id": "UniRef50_UPI0022080B54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022080B54" ], "product": "methyltransferase domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_364", "locus": "GCA013367975_00339" }, { "type": "cds", "sequence": "contig_1", "start": 184397, "stop": 184606, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTAGAGACAACGGTTTATTTGCTTATAAAAAGATTTTAAAATCTGCGTTTAATTATTTAAACGAAAAAGGAATAATTATTTTTGAAGTCGATCCTTATTGCTCTTTATGGTTTAAAAATAAATTTCCAAATGTTCTAATATTAAAAGATATAAATAACAAAGATAGAATAGCATTGCTAGCAAAAAATGATTTAAAAAATTAG", "aa": "MFARDNGLFAYKKILKSAFNYLNEKGIIIFEVDPYCSLWFKNKFPNVLILKDINNKDRIALLAKNDLKN", "aa_hexdigest": "e99f8748d453c079e19efaab39262372", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8112.4699999999975, "isoelectric_point": 9.519095420837402 }, "id": "AHCKFKLKGB_365", "locus": "GCA013367975_00340" }, { "type": "cds", "sequence": "contig_1", "start": 184641, "stop": 185753, "strand": "+", "frame": 3, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "EC:1.8.4.-", "GO:0005515", "GO:0005524", "GO:0006457", "GO:0009408", "SO:0001217", "UniRef:UniRef50_A0A448ZV77", "UniRef:UniRef90_D1J7U9" ], "nt": "ATGGAAAATAAACGTGATTATTATGAAGTTTTAGGCATTTCTAAAAATGCCACAGAAAAAGAAATCAAGTCGGCATATAGAAAATTAGCAATGCAATACCACCCAGATAGAAACAAAGCGCCAGATGCTGAAGAAAAATTTAAAGAAGTTTCAGAAGCATATGAAGTTTTATCTGACCCTGAAAAAAGACAAAAATATGATAAGTTCGGACATTCTGCATTCGACCAAAGTTCATTCGGATATTCAGAAGATGTATTTAATAGTTTCTTTAATTCTTTTAGAGATATGTTTTCTGGCGGAGGTTTTGAAGTTGATTCGAGTGATGATTCAGATGATCCTTTTTCAAGTATTTTTGGTTTTGGCTCAAATAGATCTAGAAAAACAAAGGGCAGTGATTTGCAAATGAAATTGTCAATTGATTTTGTAGATTCAATATTTGGAAAGAATGTAACTGTTCAATTAAAGAAATACAGCGAATGTTCACATTGTCATGGTTCAAGAGCAGAAAGTGATAGTGATATTGTTTCATGTCCTCAATGCCATGGTAGTGGTCAAGTTCAACAAAAAATGGGTTTCTTTTCAACAATTAGTCCTTGCAACAAGTGTGGAGGCTTGGGTAAAAGCATTAAAAACACATGCCATGTATGTAGTGGAAAAGGATATGAATATAAATTAGTGAATGAAAATATTCAAATTCCTGGCGGAATTAATAATGGCGAATATATTAAATTGTCAGGATATGGCAATCCTTCTCGCAATGGAGGATCTAATGGAGATTTATATATTATCATTGCTATTAGACCACATAAATTTTATGAAAGATTTAACAATGATATTGTAGTTTCAATGCCTATTTCTGTTTTTTCAATTATTAATGAAGATACCATTAATGTTCCTACACCATATGGAAATAAAGATATAAAATTAAATAAAAATATGAAATTAGATGGAGTAATCACTTTAGATGGCTATGGTTTTCCTATTAAAAATAGTTCTAAAAAAGGCAACTTCATTATCACATTAAAACCTTATATTCCTTCGCTTAATAAAGAAGATAATAAAAAAGTTCAAGAAATATTTAGTAACTATAAAGATGTAGAATTTCAAAAATGA", "aa": "MENKRDYYEVLGISKNATEKEIKSAYRKLAMQYHPDRNKAPDAEEKFKEVSEAYEVLSDPEKRQKYDKFGHSAFDQSSFGYSEDVFNSFFNSFRDMFSGGGFEVDSSDDSDDPFSSIFGFGSNRSRKTKGSDLQMKLSIDFVDSIFGKNVTVQLKKYSECSHCHGSRAESDSDIVSCPQCHGSGQVQQKMGFFSTISPCNKCGGLGKSIKNTCHVCSGKGYEYKLVNENIQIPGGINNGEYIKLSGYGNPSRNGGSNGDLYIIIAIRPHKFYERFNNDIVVSMPISVFSIINEDTINVPTPYGNKDIKLNKNMKLDGVITLDGYGFPIKNSSKKGNFIITLKPYIPSLNKEDNKKVQEIFSNYKDVEFQK", "aa_hexdigest": "0d17e4dae60fd352e2d4bfec96dc2669", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7U9", "query_cov": 1.0, "subject_cov": 0.9814323607427056, "identity": 0.997, "score": 734.0, "evalue": 1.13e-267, "valid": true, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "ec_ids": [ "1.8.4.-" ], "uniref50_id": "UniRef50_A0A448ZV77", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005515", "GO:0005524", "GO:0006457", "GO:0009408" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZV77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZV77" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "AHCKFKLKGB_366", "locus": "GCA013367975_00341" }, { "type": "cds", "sequence": "contig_1", "start": 185942, "stop": 186097, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAATTATGATTGTTGAATCACCAAATAAGGTTAATACTATTCAAAAGATAGTAGGTGATAACATAAAGGTATTGTCAAGTATTGGCCATATTTTAAAAATGTCAACATCAGGCATTGGAGGATTAGGTATAGATTTTGAAAATTGA", "aa": "MENKIMIVESPNKVNTIQKIVGDNIKVLSSIGHILKMSTSGIGGLGIDFEN", "aa_hexdigest": "5944c78f065504df67fff7c2f6f5aca3", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5484.369399999998, "isoelectric_point": 6.527242088317871 }, "id": "AHCKFKLKGB_367", "locus": "GCA013367975_00342" }, { "type": "cds", "sequence": "contig_1", "start": 186107, "stop": 186610, "strand": "+", "frame": 2, "gene": null, "product": "DNA topoisomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF05F56" ], "nt": "ATGGTAATTGATCCAAGCAAGAGAAAAATTGTATCCGAATTAAAAGAAGCAGCTAAAAATGCTAGTGAAGTTTTAATTGCAACTGACCCCGATCGCGAAGGCGAAGCAATTGCAAATAATTTAGTAAATACTCTAAAAGTGCAAGATAAATATAAAAGGGTAAAATACAATGAAATCACTCCAGATGCCATTAATTACGCAATTGAAAACCCTTTAACAATTGATGAAAATTTAGTAAATGCTCAAAAAGCAAGAAGAATATTAGATAGGATTATTGGTTTTAAATTGTCTCAATTAATCCAAAGAAAAGTAAAAAATACTCCAACTAATCCATCTGCTGGTCGTGTTCAATCCATCGCTTTAAAACTAGTAGTTGATAAAGAAGAAGAAATTGCAAAATTTATTCCAACATTATATTCAAAAATTGAAGCACATACTGCCGACGAACAAATTGCAAATTTCGTATATAGAGATAATTTAGATTTTGTTGGCGAAAATGAATGA", "aa": "MVIDPSKRKIVSELKEAAKNASEVLIATDPDREGEAIANNLVNTLKVQDKYKRVKYNEITPDAINYAIENPLTIDENLVNAQKARRILDRIIGFKLSQLIQRKVKNTPTNPSAGRVQSIALKLVVDKEEEIAKFIPTLYSKIEAHTADEQIANFVYRDNLDFVGENE", "aa_hexdigest": "a7316725fddc52d6a5022b04ae39801b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF0FCFB", "query_cov": 0.8203592814371258, "subject_cov": 0.8726114649681529, "identity": 0.547, "score": 138.0, "evalue": 7.02e-39, "valid": false, "product": "DNA topoisomerase", "uniref50_id": "UniRef50_UPI002FF05F56" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF05F56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF05F56" ], "product": "DNA topoisomerase" }, "genes": [], "id": "AHCKFKLKGB_368", "locus": "GCA013367975_00343" }, { "type": "cds", "sequence": "contig_1", "start": 186665, "stop": 187549, "strand": "+", "frame": 2, "gene": "topA", "product": "type I DNA topoisomerase", "db_xrefs": [ "COG:COG0550", "COG:L", "EC:5.6.2.1", "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265", "KEGG:K03168", "SO:0001217", "UniRef:UniRef50_B3PMD8", "UniRef:UniRef90_D1J7V0" ], "nt": "TTGCAGGTAATAGACTTTAAAAAATCGATTCGTTCAGAGGCAATGGTTGTTCCATTTAAACAATCTATTTTGTATAAAGAATCCCAATATAGTTCAGCAACGGTTCAAATTGCTGCACAAAAACTATTTGAAATGGGGTTAATTTCTTATCCTAGAACAGATTCAACTAGACTTTCATCAATATTTATTGAAAAATCCCAAAAATTTATTGCTCAAAAATACGGAAAAGAATATATAGCAACTGAAATAAAAGGATTTTCTGGTGATCAAGATGCCCATGAAGCAATAAGACCAACAGACATTTCACTACTTGCATCCGAAGCTCAACAAAAATTTAATTTAAGTAATGAAGAAACAAATGTATATAAATTAATCTATGAAAAGACATTAATGGCTTTGATGGCTACTCCAAAACGAGAAATATATCAATACGAATTAAAAAATGGCCAATATAATTTCAAAATGTCATATAGTAAAATAATTTTCGATGGATATTATAAAATTTTTGATAAAAAACAAGAATCTGTTATTCCTAATTATAAAGTTGGAGATATTATTAAAGTTTTAGAATTTGTTAAATTAGACAAAGAAACTAAGGCTCCTGCAAGATATAATGATGGTTCATTAATTAAGATGCTTGATGATATAAAAGTAGGAAGACCATCAACATTTGCAACGACAGTTAAAATTATAAAAGATAGACTATTTGTTGAAACTAAAAATAATAGTTTATATCCTACAGATTATGGAAAACTAGTTTTAGAAAAATTAATTCAAGGTTTTCCAACTACAATTAATGAAGAATATACAGCAAGCGTTGAAAACCAACTTGATTTAATTTCAGAAGGATTATCAGATTATAAAGTCTTATTGAAAGATTTTTGA", "aa": "MQVIDFKKSIRSEAMVVPFKQSILYKESQYSSATVQIAAQKLFEMGLISYPRTDSTRLSSIFIEKSQKFIAQKYGKEYIATEIKGFSGDQDAHEAIRPTDISLLASEAQQKFNLSNEETNVYKLIYEKTLMALMATPKREIYQYELKNGQYNFKMSYSKIIFDGYYKIFDKKQESVIPNYKVGDIIKVLEFVKLDKETKAPARYNDGSLIKMLDDIKVGRPSTFATTVKIIKDRLFVETKNNSLYPTDYGKLVLEKLIQGFPTTINEEYTASVENQLDLISEGLSDYKVLLKDF", "aa_hexdigest": "5b5b2467b045126a592db1b594e3c935", "start_type": "TTG", "rbs_motif": null, "genes": [ "topA" ], "truncated": "both", "pseudogene": { "start": 186365, "stop": 187793, "observations": { "insertion": [ 187754 ], "mutation": [ 186608, 187547 ], "stop": [ 186608, 187547 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V0", "query_cov": 1.0, "subject_cov": 0.4772727272727273, "identity": 0.993, "score": 569.0, "evalue": 7.74e-200, "gene_start": 1, "gene_end": 294, "reference_start": 242, "reference_end": 535, "reference_sequence": "MENKIMIVESPNKVNTIQKIVGDNIKVLSSIGHILKMSTSGIGGLGIDFENWEPKMVIDPSKRKIVSELKEAAKNASEVLIATDPDREGEAIANNLVNTLKVQDKYKRVKYNEITPDAINYAIENPLTIDENLVNAQKARRILDRIIGFKLSQLIQRKVKNTPTNPSAGRVQSIALKLVVDKEEEIAKFVPTLYSKIEAHTADEQIANFVYRDNLDFVGENEWIKREKVDDILAQLNQNKSLQVIDFKKSIRSEAMVVPFKQSILYKESQYSSATVQIAAQKLFEMGLISYPRTDSTRLSSIFIEKSQKFIAQKYGKEYIATEIKGFSGDQDAHEAIRPTDISLLASEAQQKFNLSNEETNVYKLIYEKTLMALMATPKREIYQYELKNGQYNFKMSYSKIIFDGYYKIFDKKQESIIPNYKVGDIIKVLEFVKLDKETKAPARYNDGSLIKMLDDIKVGRPSTFATTVKIIKDRLFVETKNNSLYPTDYGKLVLEKLIQGFPTTINEEYTASVENQLDLISEGLSDYKVLLKDFWNNFNLQLQNVNETMETDFLPQEILQEKCPLCGHDLLYRYTKSKHQKFIGCSNFPSCRYIRNIDNEKPKFLKEDIINNFYF" }, "paralog": false, "identity": 0.991578947368421, "score": 922.0, "evalue": 0.0, "description": "Internal stop codon at 186608, 187547. Frameshift due to insertion around 187754. Nonsense mutation around 186608, 187547.", "psc": { "uniref90_id": "UniRef90_D1J7V0", "gene": "topA", "product": "type I DNA topoisomerase", "ec_ids": [ "5.6.2.1" ], "uniref50_id": "UniRef50_B3PMD8", "cog_id": "COG0550", "cog_category": "L", "kegg_orthology_id": "K03168", "go_ids": [ "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V0", "EC:5.6.2.1", "UniRef:UniRef50_B3PMD8", "COG:COG0550", "COG:L", "KEGG:K03168", "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMD8" ], "product": "DNA topoisomerase 1" } }, "id": "AHCKFKLKGB_369", "locus": "GCA013367975_00344" }, { "type": "cds", "sequence": "contig_1", "start": 187562, "stop": 187780, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01396.24" ], "nt": "TTGCAACTTCAAAATGTAAATGAAACAATGGAAACAGATTTTTTACCGCAAGAAATTTTGCAAGAAAAATGCCCTCTTTGTGGACATGACTTACTTTATAGATATACTAAATCAAAACACCAAAAATTTATTGGATGTTCTAACTTTCCAAGTTGTAGATATATAAGAAATATTGACAATGAAAAGCCTAAATTTTTTAAAAGAAGATATAATAAATAA", "aa": "MQLQNVNETMETDFLPQEILQEKCPLCGHDLLYRYTKSKHQKFIGCSNFPSCRYIRNIDNEKPKFFKRRYNK", "aa_hexdigest": "e7c9e3d77905d61d1e4033d4c4c50f55", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01396.24", "name": "Zn_ribbon_Top1", "length": 40, "aa_cov": 0.5555555555555556, "hmm_cov": 0.95, "evalue": 8.25368715188846e-17, "score": 56.109832763671875, "start": 21, "stop": 60 } ], "seq_stats": { "molecular_weight": 8776.033999999992, "isoelectric_point": 9.182634925842287 }, "id": "AHCKFKLKGB_370", "locus": "GCA013367975_00345" }, { "type": "cds", "sequence": "contig_1", "start": 188098, "stop": 188241, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTATTTACAATATTGATATTTATTCAAAAACTGGAAGACCAAATACTAACGATTTAGACTTAAGAAATAAAGACAATATTTTTAAAGGATATAAAGATGTTACATTTGAATTCAGTACCGAAAATGTTCCACCTAGATGA", "aa": "MTIYNIDIYSKTGRPNTNDLDLRNKDNIFKGYKDVTFEFSTENVPPR", "aa_hexdigest": "78ee2311cc1a6af6ddc988c7f337a800", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5542.1091000000015, "isoelectric_point": 6.049965095520019 }, "id": "AHCKFKLKGB_371", "locus": "GCA013367975_00346" }, { "type": "cds", "sequence": "contig_1", "start": 188365, "stop": 190563, "strand": "-", "frame": 1, "gene": "smc", "product": "Lmp3 protein", "db_xrefs": [ "COG:COG1196", "COG:D", "SO:0001217", "UniRef:UniRef50_D1J7V1", "UniRef:UniRef90_D1J7V1" ], "nt": "ATGCAATCTGCTAAATCTTCATTAGATGCCAAAGTTACTGAAATTACTAAAAAATTAGAAACATTCAATAAAGATAAAGATGTTAAATTTAAAGAATTACAACAAACAAGAAAAAATATTGATGAATTTATAAATACAAATAAAACTAATCCAAATTATTCAACATTAATTTCAGAATTAACTTCAAAAAGAGATTCTAAAAATTCAATAACTAATTCTTCAAACAAATCAGATATTGAAACAGCAAACACTGAATTAAAACAAGCTTTAGTAAAAGCAAACACTGACAAATCTCAAGCTGATAATTTAGCAAGATCAACTAAAGAACAATTAAATAAATCAATTTCTAGTGCAAATACATTATTAGCAAAATTAACTGACAAAGATAATACAATTCAACAAGCAAAAACTGAATTAGAAAAAGAAGTTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCTGCTAAATCTTCATTAGATGCCAAAGTTACTGAAATTACTAAAAAATTAGAAACATTCAATAAAGATAAAGATGTTAAATTTAAAGAATTACAACAAACAAGAAAAAATATTGATGAATTTATAAATACAAATAAAACTAATCCAAATTATTCAACATTAATTTCAGAATTAACTTCAAAAAGAGATTCTAAAAATTCAATAACTAATTCTTCAAACAAATCAGATATTGAAACAGCAAACACTGAATTAAAACAAGCTTTAGCAAAAGCAAACACTGACAAAGCTCAAGCTGATAATTTAGCAAGATCAACTAAAGAACAATTAAATAAATCAATTTCTAGTGCAAATACATTATTAGCAAAATTAACTGACAAAGATAATACAATTCAACAAGCAAAAACTGAATTAGAAAAAGAAGTTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCTGCTAAATCTTCATTAGATACCAAAGTTACTGAAATTACTAAAAAATTAGAAACATTCAATAAAGATAAAGATGCTAAATTTAAAGAATTACAACAAACAAGAAAAAATATTGATGAATTCATAAAACAAATTGAAAATGATCCTCAAACTAAAAAGAATTATCAAAATATAGTTAAAAACTTAAAAGATAAAAAAGCTGAAAAAAATTCAATAACTTTTTCTAATAATAAAAAAGAAATTCAAGATGCAAATAAATCGTTGCAAGATGAATTAAATAATGCAAAGATTACAAAAAAAGGTATTACTGATTTTTACAACAGCAAAAAACAATTAGAAGATTTGATAAAAACTGATGATGCTAAAAAAGTAGGAACTACAGAAGCAGATACAATTTTAGATCATTATAAAAATATATCTGATGCTTCAAAAAATGAAGAAATTAAACAAGCAACCCAAAAAATAAATGATATTAAAAAAATAATAGAAACTAAAATTCAAGAAAAGAAACGTAATGAATTTTCTCAATTTGAACAAATAAAAAATGAATTGCAAAGTTTTATTAATAAAGATTTGAAGGATCAAAAATATAATTCAATTAGAACAAAAATTGAAAATAAAATAAATGGTGTTTCAAGCATAAATAAAAATTCAAAAATTCAAGATATAGAAAATGCTAAAAAACAATTAGAACCTGCTAAAAACGAGTTTGAAAAAATAATAAATAATATTAAAAAATTCAATTCCCAAAAACAAAAAGTTGAAGAAAAGTTAAAATTCTTAGATACCAAAGACATTGGTACCTCAGGTAGTCTTTCAAACAAAAACAAAGATATAAAGGCAATTTTTCAAGGTTTCATAAACGAAATAGAAAAAGAATTAATTAATAAAAAAGCTGACGATAAAGCAAAAGATGAATGCATTAATAAAATAGATTTTTTAGACAAAGCTTTAATTTCAAGATATGAAGGAATTTTAAAAGCAGCATATAAATTTTTCGACGATACATCAGTACAAAAGAATGAAGCAGAAAAAAATGAATTTTTTGATAATTTAAGAAATAATTTAAGTCAAGATAAAGTTTTTGACCAACTAGAAAAAATGAATATGAATCATTTTGAAGATTTTTATAAAACATTCTATAATAAAGGCAATGCGCCATTAAAAGATGGTAAGCCAAATTATGATGATCCAAAATATAAAGAAGAAATTAACAAACAAAATGAATTAGAAGGGCCAGCTGTAAAGTGA", "aa": "MQSAKSSLDAKVTEITKKLETFNKDKDVKFKELQQTRKNIDEFINTNKTNPNYSTLISELTSKRDSKNSITNSSNKSDIETANTELKQALVKANTDKSQADNLARSTKEQLNKSISSANTLLAKLTDKDNTIQQAKTELEKEVQKANQAVASNNTASMQSAKSSLDAKVTEITKKLETFNKDKDVKFKELQQTRKNIDEFINTNKTNPNYSTLISELTSKRDSKNSITNSSNKSDIETANTELKQALAKANTDKAQADNLARSTKEQLNKSISSANTLLAKLTDKDNTIQQAKTELEKEVQKANQAVASNNTASMQSAKSSLDTKVTEITKKLETFNKDKDAKFKELQQTRKNIDEFIKQIENDPQTKKNYQNIVKNLKDKKAEKNSITFSNNKKEIQDANKSLQDELNNAKITKKGITDFYNSKKQLEDLIKTDDAKKVGTTEADTILDHYKNISDASKNEEIKQATQKINDIKKIIETKIQEKKRNEFSQFEQIKNELQSFINKDLKDQKYNSIRTKIENKINGVSSINKNSKIQDIENAKKQLEPAKNEFEKIINNIKKFNSQKQKVEEKLKFLDTKDIGTSGSLSNKNKDIKAIFQGFINEIEKELINKKADDKAKDECINKIDFLDKALISRYEGILKAAYKFFDDTSVQKNEAEKNEFFDNLRNNLSQDKVFDQLEKMNMNHFEDFYKTFYNKGNAPLKDGKPNYDDPKYKEEINKQNELEGPAVK", "aa_hexdigest": "c86735b32bb7621a573b6d3fae96daff", "start_type": "ATG", "rbs_motif": null, "genes": [ "smc" ], "truncated": "both", "pseudogene": { "start": 188065, "stop": 190860, "observations": { "insertion": [ 190782 ], "deletion": [ 190592 ], "mutation": [ 188099, 188327, 188366 ], "stop": [ 188099, 188327, 188366, 190619, 190661, 190670, 190703, 190727, 190766, 190778 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V1", "query_cov": 1.0013661202185793, "subject_cov": 0.4610062893081761, "identity": 0.958, "score": 889.0, "evalue": 4.93e-305, "gene_start": 1, "gene_end": 732, "reference_start": 693, "reference_end": 1425, "reference_sequence": "MKKRINILMSISIVCAAASTSAIAAMCSISNNSTKKYNEAKSRLLNLIKKLDNEGQKKANDFIARQDKKFNSTAFKNHSNTSKLIDEIEIFSKKILENLQKDEKQRLEELNKLNKLRLDLQNLINSNDGQNVDSSDAKKALNENQIDDSLPIDKIKKTNENLENAKKELLNKINAERELQSKIFNEKKQELKRVLDLEDTKEVDFTKEQKVFIETNINETSSIEDIKNKIIEVEKATSSLTSKILNTKQQELQEFENIKKDLQDFINTKLNDAKYQSIKQKALDKINSLNGINKNSTIKEIKAGQNALIKAKEEAGLEKEKLDGQNIKDTLKETINNAKEFKKLLIDNDQKIVDLKSNLDNEISKAEQSLSKDKESMESANDLLNTKLIEYKEILNKFNQEKEAKFNELEQTRKNIENFLTDEVKNNPNYATLVKDLTNAKDAKKSVTNSSNKSDIIAANEALIQALADANKAKDQVDEANKSIKEQLNALIDKANTLLPQLNDNDSEIVKAKESLNAEITNANKAVNQNDNASMQSAKSSLDDKVTKIQNQLTEFNKDKDAKFKELEQTRKDIDNFLTDDVKNNPNYATLVKDLTNAKDDKKSVTKSSNKSEIIAANDELKQALDKAKVAKDQIDEANKSIKEQLSDSITNANQLLNKLVDSDKDIQKAKTELSQEIQSASQELNLNNPTSMQSAKESLDAKVTEITKKLETFNKDKDVKFKELEKTRKDIDEFINTNKTNPNYSTLISELTSKRDSKNSVTNSSNKSDIETANTELKQALAKANTDKAQADNLAKSTKEQLNNSISSANTLLAKLTDKDNTIQQAKTELEKEVQKANQAVASNNTASMQSAKSSLDAKVTEITKKLETFNKDKDVKFKELEQTRKDIDEFINTNKTNPNYSTLISELTSKRDSKNSVTNSSNKSDIETANTELKQALAKANTDKDQADNLARSTKEQLNKSISSANTLLAKLTDKDNTIQQAKTELEKEVQKANQAVASNNTASMQSAKSSLDAKVTEITKKLETFNKDKDAKFKELEQTRKDIDEFIKQIENDPQTKKNYQNIVKNLKDKKAEKNSITFSNNKKEIQDANKSLQDELNNAKITKKGITDFYNSKKQLEDLIKTDDAKKVGTTEADTILDHYKNISDASKNEEIKQATQKINDIKKIIETKIQEKKRNEFSQFEQIKNELQSFINKDLKDQKYNSIRTKIENKINGVSSINKNSKIQDIENAKKQLEPAKNEFEKIINNIKKFNSQKQKVEEKLKFLDTKDIGTSGSLSNKNKDIKAIFQGFINEIEKELINKNADDKAKDECINKIIFLDKALISRYEGILNVAYKFFGDTSTQNNQTEKNEFFDNLRNNLVDQKVFDQLEKMNMNHFEDFYNTFYNKGNAPLKPDGNPNYNDPKYLEEINKQNELEGPAVKWSAFDDILDAAFVRTAQTSFENKCKNLFNDHKKDDERYGTINKTLNVYDIDIYSKTGRADANDLDLRNKDKIFKGHEYVTFKFSTENVPGRWQKNFKGLAKWDGSKNRYRFVGIVSINLKYEYNLIISTKGENYKKPLSLEYKLGSNNKGQNYNPIIIDKISGIN" }, "paralog": false, "identity": 0.8562300319488818, "score": 1115.0, "evalue": 0.0, "description": "Internal stop codon at 188099, 188327, 188366, 190619, 190661, 190670, 190703, 190727, 190766, 190778. Frameshift due to insertion around 190782. Frameshift due to deletion around 190592. Nonsense mutation around 188099, 188327, 188366.", "psc": { "uniref90_id": "UniRef90_D1J7V1", "gene": "Smc", "product": "Lmp3 protein", "uniref50_id": "UniRef50_D1J7V1", "cog_id": "COG1196", "cog_category": "D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V1", "UniRef:UniRef50_D1J7V1", "COG:COG1196", "COG:D" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7V1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V1" ], "product": "Lmp3 protein" } }, "id": "AHCKFKLKGB_372", "locus": "GCA013367975_00347" }, { "type": "cds", "sequence": "contig_1", "start": 190705, "stop": 194523, "strand": "-", "frame": 1, "gene": null, "product": "Lmp3 protein", "db_xrefs": [ "RefSeq:WP_175933423.1", "SO:0001217", "UniParc:UPI00158E9428", "UniRef:UniRef100_UPI00158E9428", "UniRef:UniRef50_D1J7V1", "UniRef:UniRef90_Q49547" ], "nt": "ATGAAAAAAAGAATAAACATTTTAATGTCAATTTCAATCGTTTGTGCTGCTGCATCAACATCAGCCATTGCTGCTATGTGTTCAATAAGCAATAACTCCACGAAAAAGTATAACGAAGCAAAATCTAGATTACTTAATCTAATTAAGAAACTAGATAATGAAGGTCAAAAGAAAGCAAATGATTTTATTGCAAGACAAGATAAAAAATTTAATAGTACAGCATTTAAAAATCATTCAAACACATCAAAACTTATTGATGAAATAGAAATTTTTAGTAAAAAAATATTAGAAAATCTACAAAAAGATGAAAAACAAAGATTAGAAGAATTAAATAAATTAAATAAATTAAGATTAGATTTACAAAACTTAATTAATTCAAATGACGGTCAAAACGTCGATTCTTCTGATGCTAAAAAAGCATTAAATGAAAATCAAATAGATGATTCTTTGCCAATAGATAAAATTAAAAAGACAAATGAAAATCTTGAAAATGCAAAAAAAGAACTTCTAAATAAAATAAATGCCGAAAGAGAATTACAATCAAAAATTTTTAATGATAAAAAACAAGAATTAAAAAGAGTTCTTGATTTAGAAGATACAAAAGAAGTAGATTTTACAAAAGAACAAAAAGTTTTTATTGAAACAAATATAAACGAAACATCATCAATTGAAGATATAAAAAATAAAATTATTGAAGTTGAAAAAGCAACTTCTTCTTTAACATCAAAAATTTTAAATACCAAACAACAAGAACTTCAAGAATTTGAAAATATTAAAAAAGACTTACAAGATTTTATTAATACAAAATTAAATGATGCAAAATATCAATCAATAAAACAAAAAGCTTTAGATAAAATAAATAGTTTAAATGATATAAATAAAGATTCAACAATTAAAGAAATAAAAGCTGGACAAAATGCTTTAATAAAGGCAAAAGAAGAAGCAGGCCTTGAAAAAGAAAAATTAGATGGTCAAAATATAAAAGATACTCTTAAAGAAACTATAAATAATGCCAAAGAATTTAAAAAGTTATTAATTGACAATGACCAAAAAATAGTTGATTTAAAATCTAATTTAGATAATGAAATTTCTAAAGCAGAACAATCACTTTCAAAAGATAAAGAATCAATGGAATCAGCCAATGATTTATTGAATACTAAACTTATTGAATATAAAGAAATCTTAAATAAATTCAATCAAGAAAAAGAAGCAAAATTCAATGAATTAGAACAAACAAGAAAGAATATTGAAAATTTCTTAACAGATGAAGTTAAAAATAATCCAAATTATGCAACTTTAGTTAAGGATTTAACCAATGCAAAAGATGCTAAAAAATCAGTAACTAATTCTTCAAATAAATCAGACATTATTGCTGCAAATGAAGCATTGATACAAGCATTAGCAGATGCTAATAAAGCAAAAGATCAAGTTGATGAAGCAAATAAATCAATTAAAGAACAATTGAATGCATTAATTGACAAAGCAAACACATTATTGCCTCAATTAAATGATAATGATAGTGAAATTGTAAAAGCTAAAGAATCATTAAATGCAGAAATTACCAATGCAAATAAAGCAGTTAACCAAAATGACAATGTTTCAATGCAATCTGCTAAATCTTCATTAGATGACAAAGTTACTAAAATTCAAAATCAATTAACTGAGTTCAATAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGATATTGACAATTTCTTAACAGATGACGTTAAAAATAATCCAAATTATGCAACTTTAGTTAAGGATTTAACCAATGCAAAAGATGATAAGAAATCAGTAACTAAATCTTCAAATAAATCAGAAATTATTGCTGCAAAGGACGAATTAAAACAAGCCCTTGATAAGGCAAAAGTAGCAAAAGATCAAATTGATGAAGCAAATAAATCAATTAAAGAACAATTAAGCGATTCAATTACTAATGCAAACCAATTATTAAATAAATTAGTTGATTCAGACAAAGACATTCAAAAAGCAAAAACTGAATTAAGTCAAGAAATTCAAAGTGCAAGTCAAGAGCTTAATTTAAATAATCCAACATCTATGCAATCAGCAAAAGAATCTCTAGATGCCAAAGTTACTGAAATTACTAAAAAATTAGAAACTTTCAATAAAGATAAAGATGTTAAATTTAAAGAATTAGAAAAAACAAGAAAAGACATTGATGAATTTATAAATACAAATAAAACTAATCCAAATTATTCAACATTAATTTCAGAATTAACTTCAAAAAGAGATTCTAAAAATTCAGTAACTAATTCTTCAAACAAATCAGATATTGAAACAGCAAACACTGAATTAAAACAAGCTTTAGCAAAAGCAAACACTGACAAAGATCAAGCTGATAATTTAGCAAAATCAACTAAAGAACAATTAAATAACTCAATTTCTAGTGCAAATACATTATTAGCAAAATTAACTGACAAAGATAATACAATTCAACAAGCAAAAACTGAATTAGAAAAAGAAGTTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCTGCTAAATCTTCATTAGATGCCAAAGTTACTGAAATTACTAAAAAATTAGAAACTTTCAATAAAGATAAAGATGTTAAATTTAAAGAATTACAACAAACAAGAAAAAATATTGATGAATTTATAAATACAAATAAAACTAATCCAAATTATTCAACATTAATTTCAGAATTAACTTCAAAAAGAGATTCTAAAAATTCAATAACTAATTCTTCAAACAAATCAGATATTGAAACAGCAAACACTGAATTAAAACAAGCTTTAGTAAAAGCAAACACTGACAAAGCTCAAGCTGATAATTTAGCAAAATCAACTAAAGAACAATTAAATAACTCAATTTCTAGTGCAAATACATTATTAGCAAAATTAACCGACAAAGATAATACAATTCAACAAGCAAAAACTGAATTAGAAAAAGAAGTTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCTGCTAAATCTTCATTAGATGCCAAAGTTACTGAAATTACTAAAAAATTAGAAACTTTCAATAAAGATAAAGATGTTAAATTTAAAGAATTAGAAAAAACAAGAAAAGACATTGATGAATTTATAAATACAAATAAAACTAATCCAAATTATTCAACATTAATTTCAGAATTAACTTCAAAAAGAGATTCTAAAAATTCAGTAACTAATTCTTCAAACAAATCAGATATTGAAACAGCAAACACTGAATTAAAACAAGCTTTAGCAAAAGCAAACACTGACAAAGATCAAGCTGATAATTTAGCAAGATCAACTAAAGAACAATTAAATAAATCAATTTCTAGTGCAAATACATTATTAGCAAAATTAACTGACAAAGATAATACAATTCAACAAGCAAAAACTGAATTAGAAAAAGAAGTTCAAAAAGCTAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCTGCTAAATCTTCATTAGATGCCAAAGTTACTGAAATTACTAAAAAATTAGAAACATTCAATAAAGATAAAGATGTTAAATTTAAAGAATTACAACAAACAAGAAAAAATATTGATGAATTTATAAATACAAATAAAACTAATCCAAATTATTCAACATTAATTTCAGAATTAACTTCAAAAAGAGATTCTAAAAATTCAGTAACTAATTCTTCAAACAAATCAGATATTGAAACAGCAAACACTGAATTAAAACAAGCTTTAGCAAAAGCAAACACTGACAAAGATCAAGCTGATAATTTAGCAAGATCAACTAAAGAATAA", "aa": "MKKRINILMSISIVCAAASTSAIAAMCSISNNSTKKYNEAKSRLLNLIKKLDNEGQKKANDFIARQDKKFNSTAFKNHSNTSKLIDEIEIFSKKILENLQKDEKQRLEELNKLNKLRLDLQNLINSNDGQNVDSSDAKKALNENQIDDSLPIDKIKKTNENLENAKKELLNKINAERELQSKIFNDKKQELKRVLDLEDTKEVDFTKEQKVFIETNINETSSIEDIKNKIIEVEKATSSLTSKILNTKQQELQEFENIKKDLQDFINTKLNDAKYQSIKQKALDKINSLNDINKDSTIKEIKAGQNALIKAKEEAGLEKEKLDGQNIKDTLKETINNAKEFKKLLIDNDQKIVDLKSNLDNEISKAEQSLSKDKESMESANDLLNTKLIEYKEILNKFNQEKEAKFNELEQTRKNIENFLTDEVKNNPNYATLVKDLTNAKDAKKSVTNSSNKSDIIAANEALIQALADANKAKDQVDEANKSIKEQLNALIDKANTLLPQLNDNDSEIVKAKESLNAEITNANKAVNQNDNVSMQSAKSSLDDKVTKIQNQLTEFNKDKDAKFKELEQTRKDIDNFLTDDVKNNPNYATLVKDLTNAKDDKKSVTKSSNKSEIIAAKDELKQALDKAKVAKDQIDEANKSIKEQLSDSITNANQLLNKLVDSDKDIQKAKTELSQEIQSASQELNLNNPTSMQSAKESLDAKVTEITKKLETFNKDKDVKFKELEKTRKDIDEFINTNKTNPNYSTLISELTSKRDSKNSVTNSSNKSDIETANTELKQALAKANTDKDQADNLAKSTKEQLNNSISSANTLLAKLTDKDNTIQQAKTELEKEVQKANQAVASNNTASMQSAKSSLDAKVTEITKKLETFNKDKDVKFKELQQTRKNIDEFINTNKTNPNYSTLISELTSKRDSKNSITNSSNKSDIETANTELKQALVKANTDKAQADNLAKSTKEQLNNSISSANTLLAKLTDKDNTIQQAKTELEKEVQKANQAVASNNTASMQSAKSSLDAKVTEITKKLETFNKDKDVKFKELEKTRKDIDEFINTNKTNPNYSTLISELTSKRDSKNSVTNSSNKSDIETANTELKQALAKANTDKDQADNLARSTKEQLNKSISSANTLLAKLTDKDNTIQQAKTELEKEVQKANQAVASNNTASMQSAKSSLDAKVTEITKKLETFNKDKDVKFKELQQTRKNIDEFINTNKTNPNYSTLISELTSKRDSKNSVTNSSNKSDIETANTELKQALAKANTDKDQADNLARSTKE", "aa_hexdigest": "f72e15b12020b3bde9177869fd9430aa", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00158E9428", "ncbi_nrp_id": "WP_175933423.1", "uniref100_id": "UniRef100_UPI00158E9428", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E9428", "RefSeq:WP_175933423.1", "UniRef:UniRef100_UPI00158E9428" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E9428", "uniref90_id": "UniRef90_Q49547" }, "psc": { "uniref90_id": "UniRef90_Q49547", "product": "Lmp3 protein", "uniref50_id": "UniRef50_D1J7V1" }, "pscc": { "uniref50_id": "UniRef50_D1J7V1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V1" ], "product": "Lmp3 protein" }, "genes": [], "id": "AHCKFKLKGB_373", "locus": "GCA013367975_00348" }, { "type": "cds", "sequence": "contig_1", "start": 194966, "stop": 195475, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V2", "UniRef:UniRef90_D1J7V2" ], "nt": "TTGCAAATTAGTAAAAATATAAAACTTGATCCGGTTGTAATCAATTCAGCTTCTGCAAAAGAAATTTATGAAAATTTTATTCATGAAAAACAAAAAGTAGATGCGTTAAGAAATAAATATATTAATCAACACCCTGAGTTGAAACCATATTTGAATAATAATACTAGAACGGCATTTGATTTTAAATTTAAAGGTCCTAAAAAGTCTGAAATTAAAAAATTACTAAAAGATATGCCTGAAGAATTTGACGCTCATAATTTCATCTTTTCAAAAATTTTAACAAATCAAACATTTACTGAAATGAAATTCTTAAAAGTGAGATTTTCTAATTTGAAATTAGACACATCTGATAAAGAATCAAATAAACTAAAGGTAACATATGAAGTTTATTTGAATTATGAATATGCAACAGGAAGTTTCGAACTTAATAACGTTAAGGGCACTTCGCAATCTAAATATTATTTTAAAGGAAATCAAACTGTCAAGGTAATTAGTGATTCTGAAAAGTGA", "aa": "MQISKNIKLDPVVINSASAKEIYENFIHEKQKVDALRNKYINQHPELKPYLNNNTRTAFDFKFKGPKKSEIKKLLKDMPEEFDAHNFIFSKILTNQTFTEMKFLKVRFSNLKLDTSDKESNKLKVTYEVYLNYEYATGSFELNNVKGTSQSKYYFKGNQTVKVISDSEK", "aa_hexdigest": "8170ca48eca1fb592e3ddf5b37108ec0", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 194804, "stop": 195778, "observations": { "mutation": [ 194948, 195473, 195569, 195608, 195719 ], "stop": [ 194948, 195473, 195569, 195608, 195719 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V2", "query_cov": 1.0, "subject_cov": 0.49271137026239065, "identity": 0.982, "score": 327.0, "evalue": 1.22e-110, "gene_start": 1, "gene_end": 169, "reference_start": 55, "reference_end": 223, "reference_sequence": "MKNHSNTRRKHRSLAPLYYTLGSIAAAATIAGAIYFVVKNGRRVSKQYWSIEKFLQISKNIKLDPVVINSASAKEIYENFIHEKQKVDALRNKYINQHPELKPYLNNNTRTAFDFKFKGPKKSEIKKLLKDMPEEFDAHNFIFSKILTNQTFTEMKFLKMRFSNLELDTSDKESNKLKVTYEVYLNYEYATGSFELNNVKGTSQSKYYFKGNQTVKVISDSEKWNLNSKFANNLEKNLDDARKIIRIGIVKNDPKWTQSNEFREMIFKWFKRIFIENDAFPDTYSKDEYEIMPFNETSSGDDFVRWNPKKGLNNLTIKFFYVNKKNPDIKSPGRIKIFTILKV" }, "paralog": false, "identity": 0.9722222222222222, "score": 626.0, "evalue": 5.27e-231, "description": "Internal stop codon at 194948, 195473, 195569, 195608, 195719. Nonsense mutation around 194948, 195473, 195569, 195608, 195719.", "psc": { "uniref90_id": "UniRef90_D1J7V2", "uniref50_id": "UniRef50_D1J7V2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V2", "UniRef:UniRef50_D1J7V2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7V2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19830.3968, "isoelectric_point": 9.397765922546387 }, "id": "AHCKFKLKGB_374", "locus": "GCA013367975_00349" }, { "type": "cds", "sequence": "contig_1", "start": 195837, "stop": 196166, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_044730145.1", "SO:0001217", "UniParc:UPI0005CD28E7", "UniRef:UniRef100_UPI0005CD28E7", "UniRef:UniRef50_D1J7V3", "UniRef:UniRef90_D1J7V3" ], "nt": "ATGGAAATAATTAATAAAGATCTAGAGGCAAAATTAAAAACGTTTCAAAAGCAAAATTATAAATTGCAAAAAATTTGTCAATATTTTAAAATAGATTTAAAAAAACTTAATTTAAATGAATTTTTAGATTTTAATAAAAATAGTGAAAACCCTTTATTGGATTTAAAACAAATCAAACAAATTATTGCAGAAAATCAAGGTGATTCCAATATCCTTGAAGTTTTAAACATGATTAAAGAACTAGAAAGTATTAATAAAGAAAAAACTACAAATGATGAAACTTATAAACAAGATCAAATTTTAAAAAAAATTTTAAGTAATTTAGAGTAA", "aa": "MEIINKDLEAKLKTFQKQNYKLQKICQYFKIDLKKLNLNEFLDFNKNSENPLLDLKQIKQIIAENQGDSNILEVLNMIKELESINKEKTTNDETYKQDQILKKILSNLE", "aa_hexdigest": "a828eb2736d3fab7a0a2ef5aa6a9f5f6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0005CD28E7", "ncbi_nrp_id": "WP_044730145.1", "uniref100_id": "UniRef100_UPI0005CD28E7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005CD28E7", "RefSeq:WP_044730145.1", "UniRef:UniRef100_UPI0005CD28E7" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005CD28E7", "uniref90_id": "UniRef90_D1J7V3" }, "psc": { "uniref90_id": "UniRef90_D1J7V3", "uniref50_id": "UniRef50_D1J7V3" }, "pscc": { "uniref50_id": "UniRef50_D1J7V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12947.819699999985, "isoelectric_point": 6.357861900329589 }, "id": "AHCKFKLKGB_375", "locus": "GCA013367975_00350" }, { "type": "cds", "sequence": "contig_1", "start": 196237, "stop": 196431, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "ATGAAAACACAAAAGCGTATTAATATTGCAATTGATGGACCTGCTGGTGTTGGCAAAACTATTATGTCAACAATGCTAGCAAAAACATTAGGTTATAAATTTTTAAGTAGTGGTAGTTTTTATAGAGTTGTTGCCTACAATGCATTACAAAACAGACTAGATTTAAATGATGAAGAATCTATTAATAATGCATGA", "aa": "MKTQKRINIAIDGPAGVGKTIMSTMLAKTLGYKFLSSGSFYRVVAYNALQNRLDLNDEESINNA", "aa_hexdigest": "9d43ae697aa19f7caf7f9de94e3a7a66", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 54, "aa_cov": 0.84375, "hmm_cov": 1.0, "evalue": 1.8669222378922995e-17, "score": 59.0133171081543, "start": 9, "stop": 62 } ], "seq_stats": { "molecular_weight": 7040.021100000001, "isoelectric_point": 9.520900535583493 }, "id": "AHCKFKLKGB_376", "locus": "GCA013367975_00351" }, { "type": "cds", "sequence": "contig_1", "start": 196522, "stop": 196677, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "ATGGCCGCATCAGCAATAGCAAAATTTCCCTTAATAAGGGCAAAGGTTAATAAGTTTATTCAATCATTTGGTCTTAAATACAAAGGAATTATTGTTGATGGTAGAGATGCAACATATAGAATCTTACCGCAGGCAGAGGTTAAATTCTTTTTGTGA", "aa": "MAASAIAKFPLIRAKVNKFIQSFGLKYKGIIVDGRDATYRILPQAEVKFFL", "aa_hexdigest": "022ecf965105dc92e068de5a1e762d7b", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 50, "aa_cov": 0.9803921568627451, "hmm_cov": 0.98, "evalue": 6.53355311286178e-12, "score": 40.899166107177734, "start": 2, "stop": 51 } ], "seq_stats": { "molecular_weight": 5745.824500000001, "isoelectric_point": 10.21090564727783 }, "id": "AHCKFKLKGB_377", "locus": "GCA013367975_00352" }, { "type": "cds", "sequence": "contig_1", "start": 196732, "stop": 196920, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "TTGAAAGCCGACTATAATGAGGTATTAGAAGATATAAAAATAAGAGATTACAATGACACAAATAGAAAATTTGATCCATTAAAAATTAGCGAAGGCAGCATTGTTATTGACACTTCAAATATGAGCATTCAAGAAAATTTTGACGTAATGTATAAAGAAGTGCGAAAGAGGCTTGATGATGAGAAATAA", "aa": "MKADYNEVLEDIKIRDYNDTNRKFDPLKISEGSIVIDTSNMSIQENFDVMYKEVRKRLDDEK", "aa_hexdigest": "6346b732c13b72ef172b74dc59ed9267", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 53, "aa_cov": 0.8548387096774194, "hmm_cov": 1.0, "evalue": 2.3407042747210224e-12, "score": 42.355751037597656, "start": 3, "stop": 55 } ], "seq_stats": { "molecular_weight": 7417.236000000002, "isoelectric_point": 4.679520225524903 }, "id": "AHCKFKLKGB_378", "locus": "GCA013367975_00353" }, { "type": "cds", "sequence": "contig_1", "start": 196910, "stop": 197053, "strand": "+", "frame": 2, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7C7AIQ0" ], "nt": "ATGAGAAATAATATTGTTGCAATAATTGGAAAACCTAATGTCGGTAAAAGTACCTTATTTAATAAAATCATAAACAAAAGAAAATCCATTGTTTATGATACGCCCGGGGTTACTAGAGATAGATTATATGAGGATGCAATTTGA", "aa": "MRNNIVAIIGKPNVGKSTLFNKIINKRKSIVYDTPGVTRDRLYEDAI", "aa_hexdigest": "62f2c355d3a368a5bd3cf56b2c965ad1", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A7C7AIQ0", "query_cov": 0.9574468085106383, "subject_cov": 0.8035714285714286, "identity": 0.711, "score": 68.2, "evalue": 2.66e-14, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A7C7AIQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A7C7AIQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7C7AIQ0" ], "product": "GTP-binding protein" }, "genes": [], "id": "AHCKFKLKGB_379", "locus": "GCA013367975_00354" }, { "type": "cds", "sequence": "contig_1", "start": 197252, "stop": 197791, "strand": "+", "frame": 2, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "SO:0001217", "UniRef:UniRef50_Q98RC1", "UniRef:UniRef90_D1J7V5" ], "nt": "TTGTTGGCAGTAAATAAACTTGAAGGTTCAAACGTTAATTATTATGATAATTCAATTTACTCGCTTGGATTTGATGAAATATTTCCAATTTCAGCAATTCATGGTGATGGAATTGGAAATTTGTTGGATAAAATCATAGAAAAATTAAATTTTAGTGATAAAGATGTTGAATCATATTTTAAATTGGCATTGCTTGGAAAAACCAATGTTGGTAAAAGTACATTGCTTAATACTTTGGCTAATGAAGAACGTTCAATTGTTAGCAATGTCGAAGGGACAACGCGAGACTCTGTTTCGTCTCTAATAAAAATTAACGGAGAAATCTTTGAGGTCGTAGATACAGCAGGTATTAAAAGAAAAAGCAAACTAACTGAAAGTGTTGAACATTATGCTTTAATGAGAGCAAATCAATCAATTGAAGATGCAAATTTATGTCTATTGGTTTTAGATGCAACTGAAGAAGTTAGCCATTTTTCTCAAAATGTAATCGGCATAGCCTATGAATTAAAAAAACCATTAATTTTGATTGTAAATAAATGA", "aa": "MLAVNKLEGSNVNYYDNSIYSLGFDEIFPISAIHGDGIGNLLDKIIEKLNFSDKDVESYFKLALLGKTNVGKSTLLNTLANEERSIVSNVEGTTRDSVSSLIKINGEIFEVVDTAGIKRKSKLTESVEHYALMRANQSIEDANLCLLVLDATEEVSHFSQNVIGIAYELKKPLILIVNK", "aa_hexdigest": "f5b7a3286fb944d6407954e0a267f0d7", "start_type": "TTG", "rbs_motif": null, "genes": [ "der" ], "truncated": "both", "pseudogene": { "start": 196952, "stop": 198094, "observations": { "mutation": [ 197051, 197789, 197867 ], "stop": [ 197051, 197789, 197867 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V5", "query_cov": 1.0, "subject_cov": 0.40589569160997735, "identity": 0.9940000000000001, "score": 340.0, "evalue": 2.11e-114, "gene_start": 1, "gene_end": 179, "reference_start": 116, "reference_end": 294, "reference_sequence": "MMRNNIVAIIGKPNVGKSTLFNKIINKRKSIVYDTPGVTRDRLYEDAIWTGKKFRVVDTGGITIEDEDFKKQIKLQAQIAIDEANVIVFLIDGKEPLTVEDYYVASLLRKSNKHVLLAVNKLEGSNVNYYDNSIYSLGFDEIFPISAIHGDGIGNLLDKIIEKLNFSDKDVESYFKLALLGKTNVGKSTLLNTLANEERSIVSNVEGTTRDSVSSLIKINGEIFEVVDTAGIKRKSKLTESVEHYALMRANQSIEDANLCLLVLDATEEVSHFSQNVIGIAYELKKPLILIVNKWDLIEKDTNTMEKYKKDLLKKVKFVDWSPIVFISAKDNLRINKLRDTIIQVKNNISRKINTNQLNDLMATAQMIRPASSINGRRLSITFARQIDGSIPTFLLFVNNVDLAHFTYLRYIENQIRENYDFSGTPIELVLRNKNKKEERE" }, "paralog": false, "identity": 0.9921052631578947, "score": 720.0, "evalue": 2.03e-266, "description": "Internal stop codon at 197051, 197789, 197867. Nonsense mutation around 197051, 197789, 197867.", "psc": { "uniref90_id": "UniRef90_D1J7V5", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_Q98RC1", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V5", "EC:3.6.5.-", "UniRef:UniRef50_Q98RC1", "COG:COG1160", "COG:J", "KEGG:K03977", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RC1" ], "product": "GTPase Der" } }, "id": "AHCKFKLKGB_380", "locus": "GCA013367975_00355" }, { "type": "cds", "sequence": "contig_1", "start": 197879, "stop": 198232, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14714.11" ], "nt": "GTGTTTATATCAGCCAAAGATAATTTAAGAATAAATAAACTAAGAGATACTATTATTCAAGTCAAGAATAATATTTCAAGAAAAATCAATACAAATCAATTAAATGACTTAATGGCTACTGCTCAAATGATAAGACCTGCTTCATCAATAAACGGCAGAAGATTATCCATTACATTTGCAAGACAAATAGATGGATCAATTCCAACATTTTTATTGTTTGTAAACAATGTTGATTTAGCCCATTTCACTTATCTAAGATATATTGAAAATCAAATAAGAGAAAATTATGATTTCTCTGGAACTCCTATAGAGCTAGTTTTAAGAAATAAAAACAAAAAAGAAGAGAGAGAATAA", "aa": "MFISAKDNLRINKLRDTIIQVKNNISRKINTNQLNDLMATAQMIRPASSINGRRLSITFARQIDGSIPTFLLFVNNVDLAHFTYLRYIENQIRENYDFSGTPIELVLRNKNKKEERE", "aa_hexdigest": "51debaa0839e8831ce52cb33b0871601", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14714.11", "name": "KH_dom-like", "length": 81, "aa_cov": 0.6923076923076923, "hmm_cov": 1.0, "evalue": 2.332887729102812e-28, "score": 93.66259002685547, "start": 28, "stop": 108 } ], "seq_stats": { "molecular_weight": 13671.543899999982, "isoelectric_point": 9.982042884826662 }, "id": "AHCKFKLKGB_381", "locus": "GCA013367975_00356" }, { "type": "cds", "sequence": "contig_1", "start": 198235, "stop": 198555, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02826.25", "PFAM:PF02737.24", "PFAM:PF02558.23", "PFAM:PF03446.21", "PFAM:PF02153.23", "PFAM:PF03721.20" ], "nt": "ATGGCAAATATTTCGATTTTTGGAAGTGGCGCTATGGGTACTGCTGTGGCAACTATCTTAGCAAATAATGGTCATAATATTACAATATATGGCATAGATGATAGTGAGTTAAATGATCTAAAAAATGGTAGAAATACAAAATATTTTGAAGATGTAAATTTACCAAAATTTAAAGTTACAAAAGATAAAAAACAAGCGGTTGAAGATTGCAATTATATAGTTTTTGCAATTCCAACTAAGGCTTTGCCTGAAACATATAAAGAAGTAATTAAATTAATAAAAAAACAAGTCATAATAATAAATGTATCTAAAGGCTTTTGA", "aa": "MANISIFGSGAMGTAVATILANNGHNITIYGIDDSELNDLKNGRNTKYFEDVNLPKFKVTKDKKQAVEDCNYIVFAIPTKALPETYKEVIKLIKKQVIIINVSKGF", "aa_hexdigest": "029ab543a64c457495d1fc070900fcf8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02826.25", "name": "2-Hacid_dh_C", "length": 104, "aa_cov": 0.9622641509433962, "hmm_cov": 0.875, "evalue": 1.5868989968024001e-07, "score": 26.19732666015625, "start": 4, "stop": 105 }, { "id": "PF02737.24", "name": "3HCDH_N", "length": 75, "aa_cov": 0.5849056603773585, "hmm_cov": 0.9866666666666667, "evalue": 2.7698929266500182e-08, "score": 29.20623207092285, "start": 4, "stop": 65 }, { "id": "PF02558.23", "name": "ApbA", "length": 99, "aa_cov": 0.9150943396226415, "hmm_cov": 0.9797979797979798, "evalue": 1.7691685154110501e-09, "score": 32.73456573486328, "start": 4, "stop": 100 }, { "id": "PF03446.21", "name": "NAD_binding_2", "length": 104, "aa_cov": 0.9433962264150944, "hmm_cov": 0.8846153846153846, "evalue": 6.562004659749904e-10, "score": 34.553951263427734, "start": 4, "stop": 103 }, { "id": "PF02153.23", "name": "PDH_N", "length": 88, "aa_cov": 0.8301886792452831, "hmm_cov": 0.8977272727272727, "evalue": 1.1351267018883428e-07, "score": 26.760461807250977, "start": 16, "stop": 103 }, { "id": "PF03721.20", "name": "UDPG_MGDP_dh_N", "length": 86, "aa_cov": 0.7358490566037735, "hmm_cov": 1.0, "evalue": 7.55919331105506e-08, "score": 27.44905662536621, "start": 3, "stop": 80 } ], "seq_stats": { "molecular_weight": 11723.46249999999, "isoelectric_point": 8.990712547302245 }, "id": "AHCKFKLKGB_382", "locus": "GCA013367975_00357" }, { "type": "cds", "sequence": "contig_1", "start": 198586, "stop": 199218, "strand": "+", "frame": 1, "gene": "gpsA", "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0240", "SO:0001217", "UniRef:UniRef50_A0A2Z5IR65", "UniRef:UniRef90_D1J7V6" ], "nt": "ATGACGCAGGAAGCCCAAGCTAATTCATTAATAAGTGGTGTAGTTTCATTAATTGGGCCATCTTTTGCAATAGACATAATTAATAAAAATATAACTGCCGTTGACGCTGTTTCTAATAATGATAAACTAAATAAAAAAGTCCAAAAATTATTTTCAAATGATTATTTTAGAGTTTATACGCAAAAAGATGTGGCAGGAGCAGAATCAGGATCAATTTTTAAGAATATTTTAGCGATTGCTAGCGGTATGGTTGCTGGGCTTGGATATAGCACAAATAGCCAAATAGCCTTAATCACTCGAGGATTTAATGAACTAAAAAAATTTGTTTTAGCTCGTGGGGGTAAGCTGAAAACGATTTTTGGATTGTCGTGCCTGGGTGATTTAATGCTAACAGCATTGTCGGATAAATCACGAAATTACACATTTGGAAAAAATTTTTATAACAAAAATTTTGATAGTTCAAAATTAACAGTGGAAGGTCTTAGATCAATAGAAATTGTTTACGATGAATATATAAAAACAAAACTATTGGATTTGCCTATTGTAAATTCACTTTATCAAATAATATACAAGAATGCTAACCCTAATGAAATAATAATTAATTTAATGAAAAGAAAGCTAAAATCAGAATAA", "aa": "MTQEAQANSLISGVVSLIGPSFAIDIINKNITAVDAVSNNDKLNKKVQKLFSNDYFRVYTQKDVAGAESGSIFKNILAIASGMVAGLGYSTNSQIALITRGFNELKKFVLARGGKLKTIFGLSCLGDLMLTALSDKSRNYTFGKNFYNKNFDSSKLTVEGLRSIEIVYDEYIKTKLLDLPIVNSLYQIIYKNANPNEIIINLMKRKLKSE", "aa_hexdigest": "ab49fe71c6c7049995428b1e6d484967", "start_type": "ATG", "rbs_motif": null, "genes": [ "gpsA" ], "truncated": "5-prime", "pseudogene": { "start": 198286, "stop": 199218, "observations": { "mutation": [ 198553 ], "stop": [ 198553 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V6", "query_cov": 1.0, "subject_cov": 0.6422018348623854, "identity": 0.995, "score": 399.0, "evalue": 1.95e-138, "gene_start": 1, "gene_end": 210, "reference_start": 118, "reference_end": 327, "reference_sequence": "MANISIFGSGAMGTAVATILANNGHNITIYGIDDSELNDLKNGRNTKYFEDVNLPKFKVTKDKKQAVEDCNYIVFAIPTKALPETYKEVIKLIKKQVIIINVSKGFWKDSNKSCHEIMTQEAQDNSLISGVVSLIGPSFAIDIINKNITAVDAVSNNDKLNKKVQKLFSNDYFRVYTQKDVAGAESGSIFKNILAIASGMVAGLGYSTNSQIALITRGFNELKKFVLARGGKLKTIFGLSCLGDLMLTALSDKSRNYTFGKNFYNKNFDSSKLTVEGLRSIEIVYDEYIKTKLLDLPIVNSLYQIIYKNANPNEIIINLMKRKLKSE" }, "paralog": false, "identity": 0.9935483870967742, "score": 600.0, "evalue": 2.33e-220, "description": "Internal stop codon at 198553. Nonsense mutation around 198553.", "psc": { "uniref90_id": "UniRef90_D1J7V6", "gene": "GpsA", "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A2Z5IR65", "cog_id": "COG0240", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V6", "UniRef:UniRef50_A0A2Z5IR65", "COG:COG0240", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z5IR65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z5IR65" ], "product": "Glycerol-3-phosphate dehydrogenase [NAD(P)+]" } }, "id": "AHCKFKLKGB_383", "locus": "GCA013367975_00358" }, { "type": "cds", "sequence": "contig_1", "start": 199233, "stop": 199514, "strand": "+", "frame": 3, "gene": "hupA", "product": "HU family DNA-binding protein", "db_xrefs": [ "COG:B", "COG:COG0776", "RefSeq:WP_175933425.1", "SO:0001217", "UniParc:UPI00158DB030", "UniRef:UniRef100_UPI00158DB030", "UniRef:UniRef50_N9UB80", "UniRef:UniRef90_A0A454C9F8" ], "nt": "ATGAACAAGAAAGAACTACTAAGTAAAACTAGCAATGAAACAGGATTTGCACATGTATTAATCGAATCTGCATTTGATGAAATAATCAGAATAATTGCCGATGAAATAGAGGCAGGAAGAGAAGTTACAATTTCGGGATTTGGAAACTTTTCTTCTAAATTTATAAAAGGCAAGGAAAAGGTTCACAACATTACTAAAGAAATTATTAATGTTCCAGATAGATTGGATCCACGTTTCAAATTCTCAACAGATTTTAAAAAACGTATTAACAGCAAAAATTAA", "aa": "MNKKELLSKTSNETGFAHVLIESAFDEIIRIIADEIEAGREVTISGFGNFSSKFIKGKEKVHNITKEIINVPDRLDPRFKFSTDFKKRINSKN", "aa_hexdigest": "d9515837138bed30d55f97e83c569ece", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158DB030", "ncbi_nrp_id": "WP_175933425.1", "uniref100_id": "UniRef100_UPI00158DB030", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158DB030", "RefSeq:WP_175933425.1", "UniRef:UniRef100_UPI00158DB030" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158DB030", "uniref90_id": "UniRef90_A0A454C9F8" }, "psc": { "uniref90_id": "UniRef90_A0A454C9F8", "gene": "HupA", "product": "HU family DNA-binding protein", "uniref50_id": "UniRef50_N9UB80", "cog_id": "COG0776", "cog_category": "B" }, "pscc": { "uniref50_id": "UniRef50_N9UB80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UB80" ], "product": "DNA-binding histone-like protein HU" }, "genes": [ "hupA" ], "id": "AHCKFKLKGB_384", "locus": "GCA013367975_00359" }, { "type": "cds", "sequence": "contig_1", "start": 199840, "stop": 200043, "strand": "-", "frame": 1, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "COG:COG3331", "COG:R", "EC:3.1.21.10", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_A0A077L6J4", "UniRef:UniRef90_D1J7V7" ], "nt": "ATGAAAAATAAAAATAATAGAGGTATGCTTTTAGAATCTATAATAAATAACACTAATGAGTATTATTTTAAAAACGGAATTGCTTTAATTCATAAAAAAAATTTGGATATTAAATTTAAAAATGTTAAATTAGAAAAAAATAAATTACTTTTAAACGATGCAAGTATCTATTCAAAAAGCACAGTAGATTATTATGGAGTTTGA", "aa": "MKNKNNRGMLLESIINNTNEYYFKNGIALIHKKNLDIKFKNVKLEKNKLLLNDASIYSKSTVDYYGV", "aa_hexdigest": "6ba5c8dd1bc0835597e28ef2ab389482", "start_type": "ATG", "rbs_motif": null, "genes": [ "recU" ], "truncated": "5-prime", "pseudogene": { "start": 199540, "stop": 200043, "observations": { "mutation": [ 199716, 199842 ], "stop": [ 199716, 199842 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V7", "query_cov": 1.0, "subject_cov": 0.40119760479041916, "identity": 1.0, "score": 130.0, "evalue": 2.08e-37, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MKNKNNRGMLLESIINNTNEYYFKNGIALIHKKNLDIKFKNVKLEKNKLLLNDASIYSKSTVDYYGVWKGRFLAFEVKSTEQKNFEKKNIKKHQIEYLNKVLLHQGIAFWIIYFKFQNEFLLIEHANFKKCLFEEKKSYICFEALKSLGKEIKLIFPGILDFLNILK" }, "paralog": false, "identity": 0.9640718562874252, "score": 320.0, "evalue": 9.86e-115, "description": "Internal stop codon at 199716, 199842. Nonsense mutation around 199716, 199842.", "psc": { "uniref90_id": "UniRef90_D1J7V7", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_A0A077L6J4", "cog_id": "COG3331", "cog_category": "R", "kegg_orthology_id": "K03700", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V7", "EC:3.1.21.10", "UniRef:UniRef50_A0A077L6J4", "COG:COG3331", "COG:R", "KEGG:K03700" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L6J4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L6J4" ], "product": "Holliday junction resolvase RecU" } }, "id": "AHCKFKLKGB_385", "locus": "GCA013367975_00360" }, { "type": "cds", "sequence": "contig_1", "start": 200081, "stop": 200683, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "TTGAATTACGACTATAAAACAATTATGAAAGTAATAAATGATTTATATGATGAGAAAGCGGATTATTATGTCGTATATAACGACACATATGAAGCATCTAGAATAAAAGAAATGCAAGAAATTTTGGAATATTACAAAGATGATGGATTTATTAGCCAAAATGAATTTGACATTTTAAAAGAAAAATCTCAATTTATATTAAGCAAGATTATTGATATAGAAAATTTATTTTTTAGACCAGCAATGGATTGCAATATAAAAAGCTCTATTGTAAATTTAGGGCAAATAAAAAATGTCTATTCAATAAAAAAGATTGAGCTTTTTGTAAATGAAAATAAATTAAATTTAAAACATAAAATTTTTCCATATCACGAACTTGATGTAAAAAATGGTGGAATGGCATTGCAAATAGCAACGCTAAGAGCTTTAAATAAAATAAGAGATAATGAATGGACGGTCAAAGAAGCTCAATTAAAAAGATATTGCCATAACGATGTTTTGGCAATGATTATGGCATTTGACTTAGTTGAATATTTATTAAAATCAAAAAATATTTATTTTAAAAACTTCGAAACATATAAAGACTTAAAAGCGAAAAAATAG", "aa": "MNYDYKTIMKVINDLYDEKADYYVVYNDTYEASRIKEMQEILEYYKDDGFISQNEFDILKEKSQFILSKIIDIENLFFRPAMDCNIKSSIVNLGQIKNVYSIKKIELFVNENKLNLKHKIFPYHELDVKNGGMALQIATLRALNKIRDNEWTVKEAQLKRYCHNDVLAMIMAFDLVEYLLKSKNIYFKNFETYKDLKAKK", "aa_hexdigest": "15f6b59e71b21ea58c153e419201da45", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 71, "aa_cov": 0.355, "hmm_cov": 0.7746478873239436, "evalue": 1.689211849535871e-06, "score": 23.669660568237305, "start": 7, "stop": 77 } ], "seq_stats": { "molecular_weight": 23884.39450000002, "isoelectric_point": 7.641911506652832 }, "id": "AHCKFKLKGB_386", "locus": "GCA013367975_00361" }, { "type": "cds", "sequence": "contig_1", "start": 200765, "stop": 201082, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGACTTAAAAGATAATTTAAATTTAGGAATCAAATTCTACCAATTATTAGTTAAAAAGGTTATTCCTAAATACATTATTTGTCAAAAGGAATTATTAAAATTAAAAGTTAAATCATTAACTGATAATTCAATTGAAACCAAAGAAATTTTAGATTTTTATGAAAACAATTTAATTTCAATTTCACCCGATATTTTAAACAAAGATAAATGTCCTGAATATTTATTATTAAAAGAATCTAATAAAAAAATAGTTTGGTTTGATTTTGAAGGCGTTACCTTGCCCGTTCCAATGATTAACCACTCTCTAGCATGA", "aa": "MDDLKDNLNLGIKFYQLLVKKVIPKYIICQKELLKLKVKSLTDNSIETKEILDFYENNLISISPDILNKDKCPEYLLLKESNKKIVWFDFEGVTLPVPMINHSLA", "aa_hexdigest": "2fa7cce725a0224190f7059828724bc2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12227.321999999984, "isoelectric_point": 6.566972541809082 }, "id": "AHCKFKLKGB_387", "locus": "GCA013367975_00362" }, { "type": "cds", "sequence": "contig_1", "start": 201455, "stop": 202087, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATTTTAGTGAAGTTTATGAAAGCGACAATCAAGAAGACAACGGTTATGAAATGTTGGAATTTGCAAATGATGATATTGAAAACATTCTTGACAAAAATTTTAATCAATACTTTGAAAATAACTCTACTAAAAACAAAGACTTTTTAAAGCTAAATACTATTGAAAAAATAAAAAAAATTCAACAAGATATGCAAAATGATTCAACACTGTTTGAAAAAATTAATTCAGATGGTAAACTAGAATTATTTAACAAAATATTGGATAATTTTTTTCAATTTCAAATGGTTGATAGTGGAATAGCCCTTGTCGAGGAACAAATTATAAATTTTTTAACAAAAAATTCTTCAAATAAAGTAAATATAATTTCTAAAAAATCTTCAACCGATGAAAAAATTTTACAAACAAAAGAATTTTTAATGCAAAATTCGCTAATAATAAATCCTGTTTTTGAATATAAAAAATGCATATCAAAGCCTTTTTACTACGATAGTAAAACTAAAGAAATGGGAAATATTGTATATTCATCAAAAACTAGCCTAAAGGATTTATTAAGAGCATATTATGATTATTCCGTTCTTTCATCCTTAGGAATTGAAATCAATAAAATTTTCATTGTGAAACCTTGA", "aa": "MNDFSEVYESDNQEDNGYEMLEFANDDIENILDKNFNQYFENNSTKNKDFLKLNTIEKIKKIQQDMQNDSTLFEKINSDGKLELFNKILDNFFQFQMVDSGIALVEEQIINFLTKNSSNKVNIISKKSSTDEKILQTKEFLMQNSLIINPVFEYKKCISKPFYYDSKTKEMGNIVYSSKTSLKDLLRAYYDYSVLSSLGIEINKIFIVKP", "aa_hexdigest": "5961c665a6bbb5bdee6928003352ca11", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24596.562000000027, "isoelectric_point": 4.855664253234862 }, "id": "AHCKFKLKGB_388", "locus": "GCA013367975_00363" }, { "type": "cds", "sequence": "contig_1", "start": 202545, "stop": 203015, "strand": "-", "frame": 2, "gene": null, "product": "MAGa7180 family putative nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBU0", "UniRef:UniRef90_D1J7V9" ], "nt": "ATGGAAAAAAAAATAATAATTCCTAAAAGAACATTTTTTAACAATAAAAATTACACTTTGGATTATAAAAATAAAACTTTAATAGTAGATCCAAAAATCTTAAAATCTATGCAAGAAAAAAAACCTGGCACTTTTGGAGGATTTAAAAAAATGACAGGTTCGGCTTTGGGGGCTATAATGGGATTGAGCTCATTTGACAGCCAATTTGAAGCATTTTGTAATATATGCGGTTTTAGAAAACCAATATTGAACAGGAAATATGTTGATGCAGGTATTGCATTAGAACCAAAAATAGTAAAAATTATTGAAAAAAAATTAAATTCCAAATTGCTTAGATATGAAGCCAATCAATATAATTATGATTATTTTAAAGATAACGAGCTATTTGGAGGGTTGCCTGATGGCTATAGTCCCGAAAGACATTTAATTATTGAAATTAAAACTGTTGGGGAAAAAAATTATGAAAATTGA", "aa": "MEKKIIIPKRTFFNNKNYTLDYKNKTLIVDPKILKSMQEKKPGTFGGFKKMTGSALGAIMGLSSFDSQFEAFCNICGFRKPILNRKYVDAGIALEPKIVKIIEKKLNSKLLRYEANQYNYDYFKDNELFGGLPDGYSPERHLIIEIKTVGEKNYEN", "aa_hexdigest": "62555fefa421922b2ff76884a03c3221", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 202242, "stop": 203015, "observations": { "mutation": [ 202292, 202331, 202388, 202547 ], "stop": [ 202292, 202331, 202388, 202547 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V9", "query_cov": 1.0, "subject_cov": 0.5531914893617021, "identity": 1.0, "score": 318.0, "evalue": 2.92e-108, "gene_start": 1, "gene_end": 156, "reference_start": 1, "reference_end": 156, "reference_sequence": "MEKKIIIPKRTFFNNKNYTLDYKNKTLIVDPKILKSMQEKKPGTFGGFKKMTGSALGAIMGLSSFDSQFEAFCNICGFRKPILNRKYVDAGIALEPKIVKIIEKKLNSKLLRYEANQYNYDYFKDNELFGGLPDGYSPERHLIIEIKTVGEKNYENWIKYGVNPGYIKQAQLYTYLIGATSFSIVACFLKEEDYLNPNNVDITKRIIKNWTYILNNEQVQDDINYCMQWYKKYTQIGISPKWNEQIDQDLLKFLECNNQEEWKNLYLYWVDQGKAVPEYEQA" }, "paralog": false, "identity": 0.9844357976653697, "score": 511.0, "evalue": 5.73e-187, "description": "Internal stop codon at 202292, 202331, 202388, 202547. Nonsense mutation around 202292, 202331, 202388, 202547.", "psc": { "uniref90_id": "UniRef90_D1J7V9", "product": "MAGa7180 family putative nuclease", "uniref50_id": "UniRef50_A0A077LBU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V9", "UniRef:UniRef50_A0A077LBU0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077LBU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBU0" ], "product": "YqaJ viral recombinase domain-containing protein" } }, "id": "AHCKFKLKGB_389", "locus": "GCA013367975_00364" }, { "type": "cds", "sequence": "contig_1", "start": 203250, "stop": 204047, "strand": "+", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W0", "UniRef:UniRef90_D1J7W0" ], "nt": "ATGAAAAAATACACTAAAATTTTATTAACATTAGGAGCTATTTCTACTTCTATTGTTGCTGTTCCATTGGTAGCAGCAGGATGCAATGATCCAAAGAATAAAAAAAACCCAAACAATAATGATCAAAGTCAAGATGGCCAAATTGAATTTCAAGAAGGCTTAGGGTTAAAAATTGCAAAAATCGCCAAAGATCAAGAAAATGTTAAAGTTGAAGAATTTGTAAAAGAAGCAAAGAGTGCAAAGACTCTAGATCAAATAAAAGCATTATTTACTAAATATAAAATTGCTTATGATTTAAGTGAAATTCCAGATGATGCAAAATATGAAGTTGCTCCTGGTACACACGGTCATGCCGATGAAGGACTAGTACACTTAGATATTATCCAAACAGTGAATGAAAAGCAAAATAATAATAGATTTGAAATTAAAGGTTTTAAAAAGGAAACTGTTGAAGAAACAGTCCAATTGGGAGGATATAAGTTATCAACAAAAATCCTAGATAATAAAAAAGATTTTTCTTTGCAAGAAATAAAGAAACAAATTATTGACGCTCAAGAAAAAGGCTTTAATTCTTTACTTGAAAAATTAAAAGAATTTGTTAATATTGAAGTTGATGAAAATAATAAAACAAAACAATTTGAATTTAATAAAGAAAAGATTGAATTAATTAGTCAATCTGGTCAAATTGTTTTTAAAGGAATTTCTATTATTGATATTAGTAAAAAAGTAAATGAAGTCGGATATAAAACCGAAAGCAAAACTGATTTTATAATTCAAGGCTTGAACAAAGTTGAATAA", "aa": "MKKYTKILLTLGAISTSIVAVPLVAAGCNDPKNKKNPNNNDQSQDGQIEFQEGLGLKIAKIAKDQENVKVEEFVKEAKSAKTLDQIKALFTKYKIAYDLSEIPDDAKYEVAPGTHGHADEGLVHLDIIQTVNEKQNNNRFEIKGFKKETVEETVQLGGYKLSTKILDNKKDFSLQEIKKQIIDAQEKGFNSLLEKLKEFVNIEVDENNKTKQFEFNKEKIELISQSGQIVFKGISIIDISKKVNEVGYKTESKTDFIIQGLNKVE", "aa_hexdigest": "339b12539001de07143ab6a0a9c4661e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J7W0", "query_cov": 1.0, "subject_cov": 0.9814814814814815, "identity": 0.981, "score": 488.0, "evalue": 1.08e-173, "valid": true, "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J7W0" }, "pscc": { "uniref50_id": "UniRef50_D1J7W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W0" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "AHCKFKLKGB_390", "locus": "GCA013367975_00365" }, { "type": "cds", "sequence": "contig_1", "start": 204679, "stop": 205026, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTATTTCACTAGGAAATTCAAAAACTAAATTATTTAAACAAAACATTATTAAAAATTTAATTATTGCTGAATTACAAGTCATTTTGCCTTTTTATATATTGCTTTTTAGTACTTCATTAATAATTGAGAGAATTTTTTCAATACCAGGACAAAGTATTTTTATTTCTTATGTATTTACAAAAGCAGAATTAAATTGCATAATGTTTTATTTTAGTTTTAATTTTTTTGCTTTATTGATTGCTAGATTCATAAATGAAATAATTCTAAATTATTTGAATCCTGCTCAAGCTATTGAAAAGCGTATAAGATGGATTAAAAGAAAAAGGTTAGATTATGAATGA", "aa": "MFGISLGNSKTKLFKQNIIKNLIIAELQVILPFYILLFSTSLIIERIFSIPGQSIFISYVFTKAELNCIMFYFSFNFFALLIARFINEIILNYLNPAQAIEKRIRWIKRKRLDYE", "aa_hexdigest": "7e199641eb37af17c4d5ac9fde9b865b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13624.162099999976, "isoelectric_point": 9.809589958190916 }, "id": "AHCKFKLKGB_391", "locus": "GCA013367975_00366" }, { "type": "cds", "sequence": "contig_1", "start": 205019, "stop": 205687, "strand": "+", "frame": 2, "gene": "dppC", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG1173", "COG:EP", "SO:0001217", "UniRef:UniRef50_D1J7W2", "UniRef:UniRef90_D1J7W2" ], "nt": "ATGAATGAACTAGATAATAAAAAAGAACTTTTTCAATTTGCTAAAAAAAGACAACAAATTCACAATTACCCTATATTTCAAAGCGAAACAAAATTATTTTGGAAGAGATTTTTTAATAAAAAAAGCAACATCGTAATATTTAGTTTTTTATTAGCATTCATTTTACTAACATTAATATTGCTATGTTTTGTAAAATTTTCTCCAATACATTCGATTGATAATAAAAGCGATTTTGTCAATAATTTACCTCCCTATTATTCTCAAATTATTACAAGAAATTTTGAAAGAGGATATCAACTTGATTTGATAAGATCTATTGAAAATCAAGAGTCAATTAGGGCTGCTAAATTAGGTATTAAACCAATATTTAGAATCTTGTATGATTCAGCAAGAGATAGCGGGGGAAGTTTAACTGTAAATACTGATATTGTTGCTTTAACATATAATCCCTACGATTTAATAAAGGCAATAAATTTGAATCAAAATTCAATAAATATCAATATCTTAAATAAAGTTTTACTTGGAACAACAGAAAGCGGAATTGATATTTATTCAAGGATATTTTGTGCATTTGCATTAACAACCTCAATTATATTATTGGCAATATTTATTAATATTTTTATTGGGTTTTCTCTAGCAGCATTTTATGTTTTTAATCGTTACAAATGA", "aa": "MNELDNKKELFQFAKKRQQIHNYPIFQSETKLFWKRFFNKKSNIVIFSFLLAFILLTLILLCFVKFSPIHSIDNKSDFVNNLPPYYSQIITRNFERGYQLDLIRSIENQESIRAAKLGIKPIFRILYDSARDSGGSLTVNTDIVALTYNPYDLIKAINLNQNSININILNKVLLGTTESGIDIYSRIFCAFALTTSIILLAIFINIFIGFSLAAFYVFNRYK", "aa_hexdigest": "0d3fc454d370c2f736a1855d6765b6a1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "dppC" ], "truncated": "3-prime", "pseudogene": { "start": 205019, "stop": 205990, "observations": { "mutation": [ 205685, 205745, 205781, 205817 ], "stop": [ 205685, 205745, 205781, 205817 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W2", "query_cov": 1.0, "subject_cov": 0.556390977443609, "identity": 0.995, "score": 416.0, "evalue": 3.81e-144, "gene_start": 1, "gene_end": 222, "reference_start": 1, "reference_end": 222, "reference_sequence": "MNELNNKKELFQFAKKRQQIHNYPIFQSETKLFWKRFFNKKSNIVIFSFLLAFILLTLILLCFVKFSPIHSIDNKSDFVNNLPPYYSQIITRNFERGYQLDLIRSIENQESIRAAKLGIKPIFRILYDSARDSGGSLTVNTDIVALTYNPYDLIKAINLNQNSININILNKVLLGTTESGIDIYSRIFCAFALTTSIILLAIFINIFIGFSLAAFYVFNRYKWYGIVIDKIAVTLNAIPEIIWIFLLCIFLGTNWYGILIAFILISWISFYELSKNEILNLIQKEYIKAAKIIGLNNFQITYRHLFKNVLPSLIILIADRLAINILIVASLAFLDFLTAESNLNIGTIFKEALSSIKVNPQYLIIVAVYITIFCILLKLFSIGLSMTYDPKISKIINRI" }, "paralog": false, "identity": 0.9845201238390093, "score": 612.0, "evalue": 1.01e-223, "description": "Internal stop codon at 205685, 205745, 205781, 205817. Nonsense mutation around 205685, 205745, 205781, 205817.", "psc": { "uniref90_id": "UniRef90_D1J7W2", "gene": "DppC", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_D1J7W2", "cog_id": "COG1173", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W2", "UniRef:UniRef50_D1J7W2", "COG:COG1173", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W2" ], "product": "Oligopeptide transport system permease protein" } }, "id": "AHCKFKLKGB_392", "locus": "GCA013367975_00367" }, { "type": "cds", "sequence": "contig_1", "start": 206305, "stop": 206835, "strand": "+", "frame": 1, "gene": "yqdH", "product": "Iron-containing alcohol dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG1979", "SO:0001217", "UniRef:UniRef50_H3NQ88", "UniRef:UniRef90_D1J7W3" ], "nt": "ATGTTGAATTTTATTTATAAAAACGATTGTAAAATCATATTTGGAAAAGATTCAATTCAAAATTTAGAAGAAGAATTGAAATCTTTAAAAGTAAAGAAAATTTTAATTGTTTATAGTGGAAAATATATTTTTGAACTAAAAATATATGACGAAATAATTAAGGCATGCAAAAAATTAAATATAGAATTTATTGAAAATGGTAATGTTGTTCCTAACCCAAGAATTGAATTAGTAAGAAAATTAGTATTAGAAACAAAGGCTCAAAATGTTGATTTTATACTTGCCATTGGAGGTGGTTCAGTAATCGACACCGCCAAAGCAATAGCAGTTGGAGCGAAAAGCGATGTTGATGTTTGGAAATTTTATACATATGAAGATAAACCAAATTGTGCATTACCTATCGGAGTTATTTCGACAATTGCATCTAGCGGTTCCGAATCGTCAAATTGCTCAATTTTGTCAAATGAGAATCACAAACTTGGCATAGAATATGATTTTATTATTCCAAAATTTGCAATAATTGATCCTTGA", "aa": "MLNFIYKNDCKIIFGKDSIQNLEEELKSLKVKKILIVYSGKYIFELKIYDEIIKACKKLNIEFIENGNVVPNPRIELVRKLVLETKAQNVDFILAIGGGSVIDTAKAIAVGAKSDVDVWKFYTYEDKPNCALPIGVISTIASSGSESSNCSILSNENHKLGIEYDFIIPKFAIIDP", "aa_hexdigest": "a039457cc5db0f89488934a8401831ff", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [ "yqdH" ], "truncated": "3-prime", "pseudogene": { "start": 206305, "stop": 207138, "observations": { "mutation": [ 206833, 207091 ], "stop": [ 206833, 207091 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W3", "query_cov": 1.0056818181818181, "subject_cov": 0.45618556701030927, "identity": 0.9890000000000001, "score": 342.0, "evalue": 8.49e-116, "gene_start": 1, "gene_end": 176, "reference_start": 1, "reference_end": 177, "reference_sequence": "MLNFIYKNDCKIIFGKDSIQNLEGELKSLKVKKILIVYSGKYIFELKIYDEIIKACKKLNIEFIENGNVVPNPRIELVRKLVLETKAQNVDFILAIGGGSVIDTAKAIAVGAKSDDVDVWKFYTYEDKPNCALPIGVISTIASSGSESSNCSILSNENHKLGIEYDFIIPKFAIIDPWYTKSLPMYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKLLMEDPNDMNARSEIFLISIFAHNNMLDSGRVADWASHRIEHELSNFYGITHGEGMAIVMVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQDEKANLLSKKLDEFYRTIKMRTRLSELGVSCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK" }, "paralog": false, "identity": 0.9820143884892086, "score": 535.0, "evalue": 2.15e-194, "description": "Internal stop codon at 206833, 207091. Nonsense mutation around 206833, 207091.", "psc": { "uniref90_id": "UniRef90_D1J7W3", "gene": "YqdH", "product": "Iron-containing alcohol dehydrogenase", "uniref50_id": "UniRef50_H3NQ88", "cog_id": "COG1979", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W3", "UniRef:UniRef50_H3NQ88", "COG:COG1979", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_H3NQ88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H3NQ88" ], "product": "Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein" } }, "id": "AHCKFKLKGB_393", "locus": "GCA013367975_00368" }, { "type": "cds", "sequence": "contig_1", "start": 206854, "stop": 207093, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCAAATTTCATGTGGCATATCTGATATTAGTTCTCATCTAATTGAAAGATATTATACAAATATAGAACATGTTGATGCAACAGATTACATGATTGAAGGACTTTTAAAGGCATTGATGATAAATGCTAAATTATTAATGGAAGATCCAAATGATATGAATGCAAGGTCAGAAGTATTTTTAATTTCTATTTTTGCACACAATAATATGTTAGATTCTGGTAGAGTTGCTGATTGA", "aa": "MYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKLLMEDPNDMNARSEVFLISIFAHNNMLDSGRVAD", "aa_hexdigest": "23b965e3c3e0931a1d406117acaf40a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8983.178399999993, "isoelectric_point": 4.593579673767089 }, "id": "AHCKFKLKGB_394", "locus": "GCA013367975_00369" }, { "type": "cds", "sequence": "contig_1", "start": 207166, "stop": 207468, "strand": "+", "frame": 1, "gene": null, "product": "iron-containing alcohol dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H1B6A3", "UniRef:UniRef90_UPI00215D79B7" ], "nt": "ATGGTTGCATATGCAAAATATATGGCAATTGAAAAACCTAAAAAATTAGCTCAATTAGCCGAAAGAGTTTTTAATGTAGACAAGGGTTTGAGTCAAGAAGAAAAGGCTAATTTATTGTCAAAAAAATTAGATGAATTTTATAGAACAATAAAAATGAGAACTAGATTATCAGAATTAGGAGTAAGTTGCGAAAAGTTTCGTGAAATGGCATTAAGTGCAACTAAAAACGACAGTCAAACCATTGGTCATTATAAACCCCTTTTATCAAAAGAAATAATTGAAATTCTAAAGATTGCAAAGTAA", "aa": "MVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQEEKANLLSKKLDEFYRTIKMRTRLSELGVSCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK", "aa_hexdigest": "f29260e14884c0d1926e1f5fbbbbab27", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 206866, "stop": 207468, "observations": { "mutation": [ 207091 ], "stop": [ 207091 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D79B7", "query_cov": 1.0, "subject_cov": 0.425531914893617, "identity": 0.99, "score": 187.0, "evalue": 4.32e-58, "gene_start": 1, "gene_end": 100, "reference_start": 136, "reference_end": 235, "reference_sequence": "MSNENHKLGIEYDFIIPKFAIIDPWYTKSLPMYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKSLMEDPNDMNARSEIFLISIFAHNNMLDSGRVADWASHRIEHELSNFYGITHGEGMAIVMVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQEEKANLLSKKLDEFYRTIKMRTRLSELGVGCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK" }, "paralog": false, "identity": 0.98, "score": 384.0, "evalue": 2.95e-138, "description": "Internal stop codon at 207091. Nonsense mutation around 207091.", "psc": { "uniref90_id": "UniRef90_UPI00215D79B7", "product": "iron-containing alcohol dehydrogenase", "uniref50_id": "UniRef50_H1B6A3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D79B7", "UniRef:UniRef50_H1B6A3" ] }, "pscc": { "uniref50_id": "UniRef50_H1B6A3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H1B6A3" ], "product": "Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein" } }, "id": "AHCKFKLKGB_395", "locus": "GCA013367975_00370" }, { "type": "cds", "sequence": "contig_1", "start": 207521, "stop": 208708, "strand": "-", "frame": 3, "gene": "tktA", "product": "Transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J7W4", "UniRef:UniRef90_D1J7W4" ], "nt": "ATGAATTTCAAATACATTGAAGTGTCAAATGGTCAATGTCGTTCAATAATAAAAGCAATAAAAAAAGCACAAAAAATTAATCAACCTGTGTTTATTAAAATTTTAAGTCCTCAAAGCGAACAATTATTTAAAAAGAACTTTGATAATTCAATAACTCATGAAAAATTAGAAGAATTCAAAGACAAAACCTCTTTTCAAAAAACAAATTTGAGTGATTTGTATTCTGAGGTTGTTGAGTTTTATGAAAATAGATTGCAAAAAATAGAAAACAAAATAAAAACATTTAGTAATTCAAATAATTTGCAAATCTTTTTAAAAAACAATATTCGTGAAAATATTAACGATGTTGAAGAATTTAAAGAAAAAACATACATTGAAAATGCTTTAAAAATAATAAATAATTTATGTGATAAATACAAAAATTTACTAGTATTATCAAATGATATTTCTTATTTTCAAAATATTAAATCGTTAAATGGGATTTTTGCAAGCAACAATCTTGGAAGAGGAATTTTCTTAGGTAATAGAGAATTTACAATGATTAATATGGCAATGGGATTAGGTTTGCATTCAAGCATAGAAACAATTGTCATTGTAGATATTAATTATTTAGGTTATTTATTGCCAACATTACAAGTCTTAAAATATTCAAATGCAAAAATTCTTATACTATTAATCAATAATAGAAATAATTTTAGCCTAAGCAATGAGCTTAAAATATTATATTCATATGACCATATAAATTTATTGAGGCCTTGCGATTTCAATGAATTAAAAGGAGCATTTGAATTTCATTTTAATCACACAAAAAAGTCATCAATAATTTATTTAGAAAATAAAGAAATTAAAGAAATAAAGGATACTTCAAAAGTTGAATTTGAAGATGGCCCATATTACATTAGAAAAATTAGTCAAACAACAAATATTATAGCAGCAGGATCTGATCTTAGAATAGCAGAGCTTATTGCTAGAAGGTTAAATAGTTCTATTATTTCAATATCAAATATTGGAAACATTGAAAGCCTGAACTACGATAGAAATAATTCAATACTAATTGAATCTTATGAGGCATTATCTCATGAAAAAATTGCTAAATATAACATTGCATTAAATAAATTTTGCAACCTTGAAAAAAATGATGAAGAGTATATTGTAGAAAAAGTTTTAACTTCAATAGTAAAAAAATAA", "aa": "MNFKYIEVSNGQCRSIIKAIKKAQKINQPVFIKILSPQSEQLFKKNFDNSITHEKLEEFKDKTSFQKTNLSDLYSEVVEFYENRLQKIENKIKTFSNSNNLQIFLKNNIRENINDVEEFKEKTYIENALKIINNLCDKYKNLLVLSNDISYFQNIKSLNGIFASNNLGRGIFLGNREFTMINMAMGLGLHSSIETIVIVDINYLGYLLPTLQVLKYSNAKILILLINNRNNFSLSNELKILYSYDHINLLRPCDFNELKGAFEFHFNHTKKSSIIYLENKEIKEIKDTSKVEFEDGPYYIRKISQTTNIIAAGSDLRIAELIARRLNSSIISISNIGNIESLNYDRNNSILIESYEALSHEKIAKYNIALNKFCNLEKNDEEYIVEKVLTSIVKK", "aa_hexdigest": "65f90c7ad666ae36bb276b7c6f055856", "start_type": "ATG", "rbs_motif": null, "genes": [ "tktA" ], "truncated": "3-prime", "pseudogene": { "start": 207521, "stop": 209008, "observations": { "mutation": [ 208879, 208993, 209005 ], "stop": [ 208879, 208993, 209005 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W4", "query_cov": 1.0, "subject_cov": 0.6605351170568562, "identity": 0.997, "score": 743.0, "evalue": 4.84e-267, "gene_start": 1, "gene_end": 395, "reference_start": 204, "reference_end": 598, "reference_sequence": "MKNKKIDQIAIDNIKINSLNMAFSWKYSDFSSIMSGAKIFHALFGYFFNINRKNNSDVNRDRFVFFNPNILPLFYEILRLYSLISENEYLNLINNRNQNISYSNWTLDWIKSGEIFLGISGGFALGNTIAQNYLNRLDKNINHFTYWYVTERELQEGSTQEALAYAGTHNLRQLIVIYDANEIEYTVDSKSINPENIAKKVESMNFKYIEVSNGQCRSIIKAIKKAQKINQPVFIKILSPQSEQLFKKNFDNSITHEKLEEFKDKTSFQKTNLSDLYSEVVEFYENRLQKIENKIKTFSNSNNLQIFLKNNIRENINDVEEFKEKTYIENALKIINNLCDKYKNLLVLSNNISYFQNIKSLNGIFASNNLGRGIFLGNREFTMINMAMGLGLHSSIETIVIVDINYLGYLLPTLQVLKYSNAKILILLINNRNNFSLSNELKILYSYDHINLLRPCDFNELKGAFEFHFNHTKKSSIIYLENKEIKEIKDTSKVEFEDGPYYIRKISQTTNIIAAGSDLRIAELIARRLNSSIISISNIGNIESLNYDRNNSILIESYEALSHEKIAKYNIALNKFCNLEKNDEEYIVEKVLTSIVKK" }, "paralog": false, "identity": 0.9919191919191919, "score": 956.0, "evalue": 0.0, "description": "Internal stop codon at 208879, 208993, 209005. Nonsense mutation around 208879, 208993, 209005.", "psc": { "uniref90_id": "UniRef90_D1J7W4", "gene": "TktA", "product": "Transketolase", "uniref50_id": "UniRef50_D1J7W4", "cog_id": "COG0021", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W4", "UniRef:UniRef50_D1J7W4", "COG:COG0021", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W4" ], "product": "Transketolase" } }, "id": "AHCKFKLKGB_396", "locus": "GCA013367975_00371" }, { "type": "cds", "sequence": "contig_1", "start": 209003, "stop": 209218, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGGGGCAAAAATATTTCATGCTCTTTTTGGCTATTTTTTCAATATAAATAGAAAAAATAATTCTGATGTCAATAGGGATAGATTTGTTTTTTTTAATCCCAACATATTGCCACTTTTTTATGAAATTTTGAGATTATATAGTTTAATTAGTGAAAATGAATACCTTAACTTAATAAGTAATAGAAATCAAAACATTTCATATTCGAATTGA", "aa": "MSGAKIFHALFGYFFNINRKNNSDVNRDRFVFFNPNILPLFYEILRLYSLISENEYLNLISNRNQNISYSN", "aa_hexdigest": "bf272d45ac44cd0e2f7558d7c310d171", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8501.515799999992, "isoelectric_point": 9.220993614196775 }, "id": "AHCKFKLKGB_397", "locus": "GCA013367975_00372" }, { "type": "cds", "sequence": "contig_1", "start": 209307, "stop": 209591, "strand": "-", "frame": 2, "gene": null, "product": "MMB_0454 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W5", "UniRef:UniRef90_D1J7W5" ], "nt": "ATGAACTTTCGTTATTTTGTTGAACAAGATGCAATATATGAAGAATTAAACAATATTTTTAGTTCAAATAAAAATATAAAATTAATTGGAAAAATAGAAATTAAACCAAATCGCACTAAAACAAATGTTTTTATTAATTTAAGTTACAAATTGATAAACAAAACTAGTTTTGCATATGAAACAAAAAAACTAATATTTAAGATTGAACATAAAGTTTTTTCATTAATAAATGCCAAACCAAACAATATTAACTTAATATTCCAAGGTTATGAAGATGAAAAATAA", "aa": "MNFRYFVEQDAIYEELNNIFSSNKNIKLIGKIEIKPNRTKTNVFINLSYKLINKTSFAYETKKLIFKIEHKVFSLINAKPNNINLIFQGYEDEK", "aa_hexdigest": "6260e9e8055bd6afa1fc15267aea064e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7W5", "query_cov": 1.0, "subject_cov": 0.912621359223301, "identity": 1.0, "score": 187.0, "evalue": 3.63e-60, "valid": true, "product": "MMB_0454 family protein", "uniref50_id": "UniRef50_D1J7W5" }, "pscc": { "uniref50_id": "UniRef50_D1J7W5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W5" ] }, "genes": [], "id": "AHCKFKLKGB_398", "locus": "GCA013367975_00373" }, { "type": "cds", "sequence": "contig_1", "start": 209880, "stop": 210179, "strand": "-", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_Q98Q55", "UniRef:UniRef90_D1J7W6" ], "nt": "ATGATAAATGTTAACGATTTAAAACCAGGAGTTACATTTAAATTAGAAGGCAATATTTATGTAGTGCTTGAAGCAGCGCATTCAAAACAAGGCCGCGGCCAAGCAAATGTTAAAGCAAAAGTTAAAGATCTAAGAACAGGTTCAACAACAATTAAGTCATTTACTGGTGGCGAAAAAGTTGAACCAGCAATGATTGAAAAATCAAATATGGATTATCTATACAATGCAGGAGACGTTGTAGTTTTAATGGATCAAGAAACTTTCGAACAAATTGAAATACCTTCGGAACATCTTAATTGA", "aa": "MINVNDLKPGVTFKLEGNIYVVLEAAHSKQGRGQANVKAKVKDLRTGSTTIKSFTGGEKVEPAMIEKSNMDYLYNAGDVVVLMDQETFEQIEIPSEHLN", "aa_hexdigest": "4dfa5bee95f1db1ddb640fba54f22fd7", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "efp" ], "truncated": "5-prime", "pseudogene": { "start": 209622, "stop": 210179, "observations": { "mutation": [ 209882 ], "stop": [ 209882 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W6", "query_cov": 1.0, "subject_cov": 0.5351351351351351, "identity": 1.0, "score": 196.0, "evalue": 1.38e-62, "gene_start": 1, "gene_end": 99, "reference_start": 1, "reference_end": 99, "reference_sequence": "MINVNDLKPGVTFKLEGNIYVVLEAAHSKQGRGQANVKAKVKDLRTGSTTIKSFTGGEKVEPAMIEKSNMDYLYNAGDVVVLMDQETFEQIEIPSEHLNWELNFLKEGQKVIVRKFQNEVLDIELPANVNLKVVEAPDAVKGNTAQAAQKKVTLETGYVVETPLFIKEGEIVTISTETGKYTGRA" }, "paralog": false, "identity": 0.9945945945945946, "score": 358.0, "evalue": 1.07e-128, "description": "Internal stop codon at 209882. Nonsense mutation around 209882.", "psc": { "uniref90_id": "UniRef90_D1J7W6", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_Q98Q55", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W6", "UniRef:UniRef50_Q98Q55", "COG:COG0231", "COG:J", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q55" ], "product": "Elongation factor P" } }, "id": "AHCKFKLKGB_399", "locus": "GCA013367975_00374" }, { "type": "cds", "sequence": "contig_1", "start": 210567, "stop": 211076, "strand": "+", "frame": 3, "gene": null, "product": "NAD(+) synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD8376" ], "nt": "TTGCAACTTAAATTAAAGACAAAAATTGAAATTATTAATTTAAGTTCTTGCTTTAAAAAAATAGTAAAGATTGCAAATATAATAGACCAAGGTTCAATTGCAAATACTAAATCCAGATTATTTATGACAACATTATACGGTCTTGCATTCCAAAACAAAAATCTTGTGCTTGGCACAGATAATTTTGATGAATATTATTTGGGATATTTTACAAAATATGGAGACGGTGGATGCGATTTACTGCCTTTTGCTAACATAAAAAAATCTGATGTATATGCTATGGCGAGTTTGTTGGGTGTTCCACAGGAAATTATTGATAAAAAACCTAGCGCAACATTATACAAAAATCAATATGATGAAGAAGAATTAGGGTTTAAATACTCAGATTTTGAAAAATATCTATTAGATCCAACATCTGTTTCTGAGGAATTAATTAAAAAGATAAAGAATATTCACAAGCAAACTGAACACAAAAGAAAAGCAATCCCCAAGGGTCCAAAATTAAAATAA", "aa": "MQLKLKTKIEIINLSSCFKKIVKIANIIDQGSIANTKSRLFMTTLYGLAFQNKNLVLGTDNFDEYYLGYFTKYGDGGCDLLPFANIKKSDVYAMASLLGVPQEIIDKKPSATLYKNQYDEEELGFKYSDFEKYLLDPTSVSEELIKKIKNIHKQTEHKRKAIPKGPKLK", "aa_hexdigest": "6c82a6eb091e5d7e5e3d5a489affc069", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BD8376", "query_cov": 0.8757396449704142, "subject_cov": 1.0, "identity": 0.655, "score": 193.0, "evalue": 1.12e-60, "valid": false, "product": "NAD(+) synthase", "uniref50_id": "UniRef50_UPI0021BD8376" }, "pscc": { "uniref50_id": "UniRef50_UPI0021BD8376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD8376" ], "product": "NAD(+) synthase" }, "genes": [], "id": "AHCKFKLKGB_400", "locus": "GCA013367975_00375" }, { "type": "cds", "sequence": "contig_1", "start": 211637, "stop": 212266, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E586E53", "UniRef:UniRef90_UPI001E586E53" ], "nt": "TTGTACCAAGTAGTTGTTGAAAAAAAGGCAAATGATATCCTAGCAAAAAACTATATTTGTGAAGATGTAACTTTTAAAATATCTGCTTCAAAATCTTTAAAATCAAATACTTTAGTATCTAATACGATGCTAAAAAAATATCCAATATTTATCAAAATAAATAGAGATGAAAAAAGCAATAAATTATCAATCACTCTCTCAATTCATAGATTGATTTATGAAGTTGCTACAAAAAAAGAAATTTCAATAAATAAAAAATCTCTAAATTTGTCGATTTATCATAAAACAAATGATGAATATATTCACTTTAAAATTGATAAATATGAAGATGGAAATCCTATATTTTTAGAAAAAAAACTGATAGTAAAAATTGATGCTTATACAAAAATTAGAGATTATTTGAGCTATAAAGTTGGGGCTAATATTCTTATTAACTATGACTTAGCAAATAACCTATCAGATAATAGAAATATAGACTCAGATTTTAAAACAAGAACCCTAAAAAATATTGATATTTTTCTTACTGACAAGAGTTTTAAAGAAGATGTTAATAATTCTTCTACAACTATAGTTAAAGAATTTTTTGTTACATATTTTAAATTCAATCAACTAAAACAAAATATGCCCTGA", "aa": "MYQVVVEKKANDILAKNYICEDVTFKISASKSLKSNTLVSNTMLKKYPIFIKINRDEKSNKLSITLSIHRLIYEVATKKEISINKKSLNLSIYHKTNDEYIHFKIDKYEDGNPIFLEKKLIVKIDAYTKIRDYLSYKVGANILINYDLANNLSDNRNIDSDFKTRTLKNIDIFLTDKSFKEDVNNSSTTIVKEFFVTYFKFNQLKQNMP", "aa_hexdigest": "b7b18ef0d77f78c6238e09ffd7dc391a", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI001E586E53", "query_cov": 1.0, "subject_cov": 0.8293650793650794, "identity": 0.981, "score": 401.0, "evalue": 1.14e-140, "valid": true, "uniref50_id": "UniRef50_UPI001E586E53" }, "pscc": { "uniref50_id": "UniRef50_UPI001E586E53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E586E53" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24467.96350000002, "isoelectric_point": 9.390287590026855 }, "id": "AHCKFKLKGB_401", "locus": "GCA013367975_00376" }, { "type": "cds", "sequence": "contig_1", "start": 212620, "stop": 212883, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W9", "UniRef:UniRef90_D1J7W9" ], "nt": "ATGCGTCGTAGTAGCAACAGAGCATTCTTTTTAAAATGTTTTAATTATTTTAAGGAAATAATAAAAGAGTTAAGGGAACGAAAAATTAAAATAGATATAAATAAAATTCCCGAAATTTTTAATAAAGAGAATTTAATTTCACTTAAATTCTTAATTCAAAAATATGTTTTAAAAACAGCAAAATTGTTTGAATCTTCATTGAGTGATGATTTGAAATGTATTTATATTAAATATTTTAAAGAGCTTAAAGATGATATTAAGTGA", "aa": "MRRSSNRAFFLKCFNYFKEIIKELRERKIKIDINKIPEIFNKENLISLKFLIQKYVLKTAKLFESSLSDDLKCIYIKYFKELKDDIK", "aa_hexdigest": "8c8b63501e8df17ceedf37253533e467", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 212536, "stop": 212883, "observations": { "mutation": [ 212553, 212622 ], "stop": [ 212553, 212622 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W9", "query_cov": 1.0, "subject_cov": 0.7565217391304347, "identity": 0.9890000000000001, "score": 166.0, "evalue": 6.83e-52, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MRRSSNRAFFLKCFNYFKEIIKELRERKIKIDINKIPEIFNKENLISLKFLIQKNVLKTAKLFESSLSDDLKCIYIKYFKELKDDIKWTDFFFSKSSYYRKLNYLILAIAWFLIF" }, "paralog": false, "identity": 0.9739130434782609, "score": 218.0, "evalue": 1.31e-74, "description": "Internal stop codon at 212553, 212622. Nonsense mutation around 212553, 212622.", "psc": { "uniref90_id": "UniRef90_D1J7W9", "uniref50_id": "UniRef50_D1J7W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W9", "UniRef:UniRef50_D1J7W9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10646.579699999987, "isoelectric_point": 9.666018867492678 }, "id": "AHCKFKLKGB_402", "locus": "GCA013367975_00377" }, { "type": "cds", "sequence": "contig_1", "start": 213225, "stop": 213404, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "TTGGATGTTTTAGTTTATTCTTCAGAAAGTTTTTTACCCGGAACATCATTAAATGCAAGACTAATTGGTGCAATGAAGATGATTGATGGTGGCGAAACAGATACTAAATTAATTGCAGTTCACGCAGATGATTATAGATTAGATAATATTAAATCATTGGCAGATGTCGATCAAATGTGA", "aa": "MDVLVYSSESFLPGTSLNARLIGAMKMIDGGETDTKLIAVHADDYRLDNIKSLADVDQM", "aa_hexdigest": "2761b4dabd5cd8b4bf0800825a52388c", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 58, "aa_cov": 0.9830508474576272, "hmm_cov": 1.0, "evalue": 1.320103394183102e-17, "score": 59.326881408691406, "start": 1, "stop": 58 } ], "seq_stats": { "molecular_weight": 6446.274400000002, "isoelectric_point": 4.331608772277833 }, "id": "AHCKFKLKGB_403", "locus": "GCA013367975_00378" }, { "type": "cds", "sequence": "contig_1", "start": 213417, "stop": 213572, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAAAAATTTAGAAGTAAACATTGAAATTAGTAAAGATTCAAAAATCAAATATGAATATGACCGCAAAAGCAACAAAATCAAAGTTGACAGAATTTTAAGAGGTGATTTTAAATATCCTGCAAATTATGGCTATTTATCTGATGCACTAGATTGA", "aa": "MKNLEVNIEISKDSKIKYEYDRKSNKIKVDRILRGDFKYPANYGYLSDALD", "aa_hexdigest": "cc1867663a18a93573bdd0ed9b325cc6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 44, "aa_cov": 0.8627450980392157, "hmm_cov": 1.0, "evalue": 2.3782334264004237e-13, "score": 45.507972717285156, "start": 7, "stop": 50 } ], "seq_stats": { "molecular_weight": 6058.826600000001, "isoelectric_point": 8.92521266937256 }, "id": "AHCKFKLKGB_404", "locus": "GCA013367975_00379" }, { "type": "cds", "sequence": "contig_1", "start": 213701, "stop": 213973, "strand": "+", "frame": 2, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "SO:0001217", "UniRef:UniRef50_B3PMK3", "UniRef:UniRef90_D1J7X1" ], "nt": "ATGAAAATATATTTATATGGAAAAATAATGCACGTAAACACTAACTATTTAATTTTAGATCATAATGGTGAGGGCGAATTGATATACGTGGCGGATATAAAAAGATTTAATAAGGATGATTTGAGAAAAATATTTATTTACGATGTGGTAAATGAATATAAAAAAACTACTTATGGATTTGAAAATTTTAAAGAATTAATAATTTTTGAGGATTTAATTTCATTACAAGGTTTAGGACCTAAGACAGCAATTTCTCTTCTAAATTTAGGATGA", "aa": "MKIYLYGKIMHVNTNYLILDHNGEGELIYVADIKRFNKDDLRKIFIYDVVNEYKKTTYGFENFKELIIFEDLISLQGLGPKTAISLLNLG", "aa_hexdigest": "6ab85d8a3108cf0df2cf20e20a0b18c2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ruvA" ], "truncated": "3-prime", "pseudogene": { "start": 213701, "stop": 214276, "observations": { "mutation": [ 213971 ], "stop": [ 213971 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X1", "query_cov": 1.0, "subject_cov": 0.4368932038834951, "identity": 1.0, "score": 183.0, "evalue": 2.72e-57, "gene_start": 1, "gene_end": 90, "reference_start": 1, "reference_end": 90, "reference_sequence": "MKIYLYGKIMHVNTNYLILDHNGEGELIYVADIKRFNKDDLRKIFIYDVVNEYKKTTYGFENFKELIIFEDLISLQGLGPKTAISLLNLGWENLIQLIANEDKDKLCEASYVSLRIANNIIFAYKQKYAKFLSKLSDEDLLKIKSKNTSDSLREKFEYTMRMLGFKKQQISYALEKMSITENIEESVENAIKIIGMKQNESRVLDQ" }, "paralog": false, "identity": 0.9895287958115183, "score": 370.0, "evalue": 3.07e-133, "description": "Internal stop codon at 213971. Nonsense mutation around 213971.", "psc": { "uniref90_id": "UniRef90_D1J7X1", "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_B3PMK3", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X1", "EC:3.6.4.12", "UniRef:UniRef50_B3PMK3", "COG:COG0632", "COG:L", "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK3" ], "product": "Holliday junction branch migration complex subunit RuvA" } }, "id": "AHCKFKLKGB_405", "locus": "GCA013367975_00380" }, { "type": "cds", "sequence": "contig_1", "start": 214178, "stop": 214321, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07499.19" ], "nt": "ATGAGAATGCTCGGTTTTAAAAAACAACAAATTTCATACGCATTAGAAAAAATGAGTATAACCGAAAATATTGAAGAATCAGTTGAAAATGCAATAAAAATAATTGGAATGAAGCAAAATGAGTCAAGAGTTCTCGATCAATAG", "aa": "MRMLGFKKQQISYALEKMSITENIEESVENAIKIIGMKQNESRVLDQ", "aa_hexdigest": "4d3fcfe438bc4408c6a51f35a9623a7f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07499.19", "name": "RuvA_C", "length": 31, "aa_cov": 0.6595744680851063, "hmm_cov": 0.9354838709677419, "evalue": 1.2155643832722467e-06, "score": 24.261507034301758, "start": 3, "stop": 33 } ], "seq_stats": { "molecular_weight": 5473.3271, "isoelectric_point": 6.155799293518065 }, "id": "AHCKFKLKGB_406", "locus": "GCA013367975_00381" }, { "type": "cds", "sequence": "contig_1", "start": 214296, "stop": 215249, "strand": "+", "frame": 3, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.12", "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378", "RefSeq:WP_012855462.1", "SO:0001217", "UniParc:UPI0001BC2154", "UniRef:UniRef100_D1J7X2", "UniRef:UniRef50_D1J7X2", "UniRef:UniRef90_D1J7X2" ], "nt": "ATGAGTCAAGAGTTCTCGATCAATAGTTTTGATGAATTTGTAGGCCAAGAAAAACTAATTTCCACTATTAAAATAATAATAGAATCTGCAAAGGTTCAAAAAAAACAAATTGATCATTTACTATTTTATGGCCCGCCAGGTTTAGGTAAAACTACACTTGCTAAAATAATTTCAAAAGAAACAAAAAGCAATATTGTTTATGTTCAAGGTCCATTGATCGAAAAAAAGAGCGATGTATTGACTATTTTTTCATCAATAAATGATAATGACATCATTTTTATTGATGAAATACATGGCATAAACAAAAATATTGAAGAATTATTTTATTCAGCATTAGAAGAGGGTACAATTGACGTTGCCTTGGGCGTTGATGGTGATAAAAAAATCATGAGAATGAAATTAAAAAAATTTTGTTTGATTGCGGCCACGACAAAAATCAATTTGTTATCCAAACCCTTAAAAGATCGTTTTGGTTATATAGGAAAATTAAGCGATTATAGCGATTTAGAAATTGCTAAAATAATTTTTAACTCTGCAACTAAAAATAAGATAAAAATTAAAAATGATGCGATTAATTTTATTGCAATTCATTCAAGGCAAACACCAAGAGTCGCTAATACTCTTTTAAAAAGAGTTAATGATTTTGCCATATATTACAAAATTGATCTAATTGACTTGGAAACTGTTAAGAAAGCTTTTTCGTATTTGGGTATTTATTTGTATGGTTTGTATTCGCCTCAAATTGAATATTTAAAAACATTGCATAAAGTATTTAATGACAAATTTTCTTCGCTTGATGCGATAAGTTCAATAATTAAGGATGATAAATATACTATTTTAAATGATATTGAGCCTATATTATTGATTTATAAGTTAATTGAGAAATCACCCCGTGGTAGAAAAATTACAAACAATGGCATTGAATATTTAAAGAGTCAAAATATAATTACTTAA", "aa": "MSQEFSINSFDEFVGQEKLISTIKIIIESAKVQKKQIDHLLFYGPPGLGKTTLAKIISKETKSNIVYVQGPLIEKKSDVLTIFSSINDNDIIFIDEIHGINKNIEELFYSALEEGTIDVALGVDGDKKIMRMKLKKFCLIAATTKINLLSKPLKDRFGYIGKLSDYSDLEIAKIIFNSATKNKIKIKNDAINFIAIHSRQTPRVANTLLKRVNDFAIYYKIDLIDLETVKKAFSYLGIYLYGLYSPQIEYLKTLHKVFNDKFSSLDAISSIIKDDKYTILNDIEPILLIYKLIEKSPRGRKITNNGIEYLKSQNIIT", "aa_hexdigest": "2222fa95e3b47663b8bdd442e2d1d476", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2154", "ncbi_nrp_id": "WP_012855462.1", "uniref100_id": "UniRef100_D1J7X2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2154", "RefSeq:WP_012855462.1", "UniRef:UniRef100_D1J7X2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7X2", "uniref90_id": "UniRef90_D1J7X2" }, "psc": { "uniref90_id": "UniRef90_D1J7X2", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_D1J7X2", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7X2" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "AHCKFKLKGB_407", "locus": "GCA013367975_00382" }, { "type": "cds", "sequence": "contig_1", "start": 215411, "stop": 215965, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTCCGCAACTTAAAAAACAATCCAATTCAAATGTTACATCTGCTAAAATTGGGTTAAAAATAGAAAGGGAAAATCAAAAAGAAAACTCGTATAAAAAATCATATGGATTATCAGATCAAGAAATTTTGAATTTGACTAAGAATTACATAGGAAACCAAAACGGGGTTTTAAGTACTTCATATGATATTAATTTGGTATCTAAAAATTTGATTTCTGCTACTAGTTATCAAGATAAAACAGATAAGGATGTTAATAATTTAATATCCAATTTTGTTAATAAGCCTTATTTAACAGTAACTGATGGTGGCGGTAATCCATTATTTTATAAAGGTGAATATGTTTCGAGGTTTTATCCAAATCCTGATGTTCCTAGAACTCTTAAAGATTTTTTAAAAAATGGACCTCAAAACTACAATATTAGTGTAGAAAAAAATCCGGCAACAACCTACAACAAATTTGGTGCAAGACAAAAAATTCAAGTTAAATTGGATGAATATGGATGGGATAGTTTTGTGCAAATGATAAACGAGTACAGGCTTGCAACAAGTTGA", "aa": "MFPQLKKQSNSNVTSAKIGLKIERENQKENSYKKSYGLSDQEILNLTKNYIGNQNGVLSTSYDINLVSKNLISATSYQDKTDKDVNNLISNFVNKPYLTVTDGGGNPLFYKGEYVSRFYPNPDVPRTLKDFLKNGPQNYNISVEKNPATTYNKFGARQKIQVKLDEYGWDSFVQMINEYRLATS", "aa_hexdigest": "1ce245a0de981bfa4387d8bdadfc9cb8", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20994.258899999993, "isoelectric_point": 9.267217445373536 }, "id": "AHCKFKLKGB_408", "locus": "GCA013367975_00383" }, { "type": "cds", "sequence": "contig_1", "start": 216233, "stop": 216880, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATCAGCAGTTAACCCTGCAACATCTAGAATACTTTCAACTGAAAAAAATGAATCATCATTTTATTTGGCAAACGAAAATCTTGAAGGTTTAAGTGATGAATATATTGCTGCTGCAATTAACTATTCATATGCCCCATTTAAATTAACTAAAACTTACAATTATTTTTTAACTGGTAGTTCAACAAATCAAAATAGATATTTAGCAGTTTTAGCAATTTTATTTTCATTGTTTGCAGTATTTTTGATCGCAAAATATCGTTTATATGGAATCGTATCATCAATATGCTTGGCATTTATGATATTTGTGTTTTTATCAATTATTACTTCATTTAATATATATATAACGGGAGCGGTAGCATTTACTTTCTTAATTTCTACATTCTTAACATTTTTAATGATTTGGCAAATATTAAGACGTCTAGATAAAGAAGTAAATTCAGGTTCTAACGGAATAAAAGCAACAAATAAATCATTAAGGTTTGGAATAATATCAACACTTGATATTGCCTTTGCATTAATTGTGGTTTCAATATTTACTATTTTTATAAAAACTTCATATTTAAATGCGATGGGCATAATGCTATTTATTGGATTGGTTTCTGGATTGATTATTGGAGTATTGGTCAATGCGTTAATAATGTGA", "aa": "MISAVNPATSRILSTEKNESSFYLANENLEGLSDEYIAAAINYSYAPFKLTKTYNYFLTGSSTNQNRYLAVLAILFSLFAVFLIAKYRLYGIVSSICLAFMIFVFLSIITSFNIYITGAVAFTFLISTFLTFLMIWQILRRLDKEVNSGSNGIKATNKSLRFGIISTLDIAFALIVVSIFTIFIKTSYLNAMGIMLFIGLVSGLIIGVLVNALIM", "aa_hexdigest": "d5453b79c25ec13af73c085343245128", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23865.97420000002, "isoelectric_point": 9.32478771209717 }, "id": "AHCKFKLKGB_409", "locus": "GCA013367975_00384" }, { "type": "cds", "sequence": "contig_1", "start": 216953, "stop": 217150, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGCAAAGATTGATTTAATCTCAAAATCAAAATATTTTAGTATAGGTTTTGCAATATTTGTAATATTAACCATTGTATTCTATGGAATATATAGCGGTGTATTTGGAAGTACAAAATTAGGAATTAATGCTAGTGAAACATTAGCAAATAATTACTTATATTCTATTCAAAAATCATCAATAAATGGTGATTGA", "aa": "MIAKIDLISKSKYFSIGFAIFVILTIVFYGIYSGVFGSTKLGINASETLANNYLYSIQKSSINGD", "aa_hexdigest": "d0487408b15302ee7b0e4c118ae259b1", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7107.177599999999, "isoelectric_point": 9.050023460388182 }, "id": "AHCKFKLKGB_410", "locus": "GCA013367975_00385" }, { "type": "cds", "sequence": "contig_1", "start": 217168, "stop": 217566, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATTGTCCATGGCAAATTATTAATCAAACTATTGAACTTATAACTGCTAATATTTCGTTTATTCACATTACAAAAAACGCTATTAAACCAAATCTTATAATGCTATATATTCCACAAATTATTAATGAAACCACAATTATTAAAATAGTAAATCCAAAATTGATTAATTCACCATTTGCATTTAATGATATTTCGTGTATGTTCAAATCAAATTCATTACCTATAGCTGAAATTTTAGTATTAATAAATGTCTTTAAAGCTTCTAATTTAGTTGAATCAATCTTGTCCGCTGAAGAAATTAATAAATTATATGAGTCGTTAGTTAAACTTGATTTTTCAAAGAAAATATTTGCATTTGAAATATTTAATTCTTTTCAATATTTAATAATTTGA", "aa": "MLNCPWQIINQTIELITANISFIHITKNAIKPNLIMLYIPQIINETTIIKIVNPKLINSPFAFNDISCMFKSNSLPIAEILVLINVFKASNLVESILSAEEINKLYESLVKLDFSKKIFAFEIFNSFQYLII", "aa_hexdigest": "80462d273146f0136045a8b9f01d3483", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15122.824199999985, "isoelectric_point": 6.558730888366699 }, "id": "AHCKFKLKGB_411", "locus": "GCA013367975_00386" }, { "type": "cds", "sequence": "contig_1", "start": 217583, "stop": 217888, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTATCTACTGCATTTAGTGTAGTTGATTCTATCTCTAATTCTCAAACAATTAAGGAAGAATTTGGCAATAATTTAAATACAAAAAATTACATATTTGAAAATGACCAAATAAACAACATATTTAAAAAATATGTAAAGGAATCTTTGAATAAAAACATCTTCAATATATTCTTTGCAATTATTTTTGTGGCTTCTACATATACATTGATGACGGGCTTTAATGCAACAACAATATTAGTAACTTCAATGTTTATTATTTCAAATTCATTTATCAATTTATTTATATTACCAAACATTTGA", "aa": "MILSTAFSVVDSISNSQTIKEEFGNNLNTKNYIFENDQINNIFKKYVKESLNKNIFNIFFAIIFVASTYTLMTGFNATTILVTSMFIISNSFINLFILPNI", "aa_hexdigest": "d59ab9b7ad89d1f922ef06318eb63ce7", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11525.187799999985, "isoelectric_point": 6.089752388000488 }, "id": "AHCKFKLKGB_412", "locus": "GCA013367975_00387" }, { "type": "cds", "sequence": "contig_1", "start": 218020, "stop": 219297, "strand": "+", "frame": 1, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "COG:COG0124", "COG:J", "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "KEGG:K01892", "RefSeq:WP_175933431.1", "SO:0001217", "UniParc:UPI001588D769", "UniRef:UniRef100_UPI001588D769", "UniRef:UniRef50_A0A3Q9VA72", "UniRef:UniRef90_D1J7X4" ], "nt": "ATGTTTCAAAGACTTAAAGGCACAAGAGATATATATGGGTTAGAAGAAAAGGTTTTGAATTTTATAGAAAATAAATTTTTTAAAATTATAAAAAATTACAATTTTAAAAGAATTTGCACACCGATAATTGAAGACGCTAACTTGTTTATAAGATCAGTTGGTGAAACTAGTGACATTGTTTCAAAAGAGATGTATCTGTTTAAAGATAATGGCCAAAGAGACATTGCCTTGAGGCCAGAAGGAACAGCCTCAACCATAAGAGCTTTTGTGGAAAACAAAATAAACAATTTGGAGCCTACTAAATTAGCCTATTTTGGACCTATGTTTAGATACGAACGTCCTCAAAAAGGTCGCTATAGACAATTTATTCAAGGAGGAGTTGAATTAATTGAAAATTCAACTGTTGAGAATAATTTTGAAATAATAAAATTGGCATATGATTTTTTAAAGTCGTTAAAAATTAGTAACATTACTTTAGAAATAAACAATTTAGGTTCTTTTGAGTCAAGAAATAACTACATAGAAATATTAAAGGAATATTTTTCTAAATACAAGGAACAATTGAGTGAAATTTCTTTAATTAGATTGCAAAAGAATGTTTTGAGAATCTTAGATGATAAAGAAGAAATAAAAAAAGATTTTGTAAAAAATGCTCCAAGATTAATAGATTATTTATCTTCAATTGAAAAAGAAAGATTTAACAATTTACTAGATTTACTAGATGAATTTGAAATCAAATATGTAATAAATCCTTACTTAGTTAGGGGACTTGATTACTACAATGATGTTGTTTTTGAATTTGTTTCAAATTCAGACGCTCTAGGTTCACAATGTACTGTTCTTGGCGGCGGAAGATATGATGGCATGATTAAAAGTTTTGGCGGACCTGATATAGGTTCTATTGGGTTTGCTTTTGGAGTAGATAGATTAATGGAAATAATAATGTCAAATATTGACAATTATCCAGAGCTAAGTTTTAATGAAACGATATTAATTGCTTATTTGAATGAAGATGAAAAAAATGAAATAAGAAAAATTGCTTATGATTTGAGAAAGAATTTTCAAGTAATTTTTCCAAGTCAAAAACAATCAATTAAGAGTTTGTTTAAACTTCAATATAAATTGCAACCAAAATATTTAATTTTTAAAGAATTAAATATGAAAAAAAATGAGTTGAAAATAAAATCAGTTTTAAATGAAAGAAATATAATTTATACTAATTTAGACGAATTTAAAAATATTATTAGTGAAATGAAAAGAGATGAAAACAATGAATAA", "aa": "MFQRLKGTRDIYGLEEKVLNFIENKFFKIIKNYNFKRICTPIIEDANLFIRSVGETSDIVSKEMYLFKDNGQRDIALRPEGTASTIRAFVENKINNLEPTKLAYFGPMFRYERPQKGRYRQFIQGGVELIENSTVENNFEIIKLAYDFLKSLKISNITLEINNLGSFESRNNYIEILKEYFSKYKEQLSEISLIRLQKNVLRILDDKEEIKKDFVKNAPRLIDYLSSIEKERFNNLLDLLDEFEIKYVINPYLVRGLDYYNDVVFEFVSNSDALGSQCTVLGGGRYDGMIKSFGGPDIGSIGFAFGVDRLMEIIMSNIDNYPELSFNETILIAYLNEDEKNEIRKIAYDLRKNFQVIFPSQKQSIKSLFKLQYKLQPKYLIFKELNMKKNELKIKSVLNERNIIYTNLDEFKNIISEMKRDENNE", "aa_hexdigest": "2a87df07e59e1f20dd90c23d6ffe34b6", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI001588D769", "ncbi_nrp_id": "WP_175933431.1", "uniref100_id": "UniRef100_UPI001588D769", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588D769", "RefSeq:WP_175933431.1", "UniRef:UniRef100_UPI001588D769" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588D769", "uniref90_id": "UniRef90_D1J7X4" }, "psc": { "uniref90_id": "UniRef90_D1J7X4", "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_A0A3Q9VA72", "cog_id": "COG0124", "cog_category": "J", "kegg_orthology_id": "K01892", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3Q9VA72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3Q9VA72" ], "product": "Histidine--tRNA ligase" }, "genes": [ "hisS" ], "id": "AHCKFKLKGB_413", "locus": "GCA013367975_00388" }, { "type": "cds", "sequence": "contig_1", "start": 219422, "stop": 220033, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "GTGCAATTAATATTTCAAAATGTATCAGATCCTTTATTAACTAAAGAAAGTTGTATTTATGTAAAAGGAACTGTTCAAAAGCGTTTAGAAGTTAATCCAGCATTAAAAACCGGAGAAATCGAAGTTATAGTTTCTGATTATAAAATTTTTAATTCAGCAAAGCAAATACCTTTTGAAATTAACAATTCAAAATCAACAAATGATGCAAACGAAGATATAAGATTGGAATATCGTTTTCTTGATTTAAGAGAAGAAAGAATGCTTAACAATTTAAGATTAAGACATAAATTCTTACTTTTGGTAAGAAATTTCTTTGATAAACAAGGCTACATTGAAGTAGAAACCCCAATTTTGGGTAAATCAACTCCTGAAGGAGCTAGAGATTACTTGGTACCCACAAGAAGAAAGGGAAGATTTTTTGCATTACCTCAATCACCACAACTATTTAAGCAATTATTGATGGCTGCTGGATTTGAAAAATATTTCCAAATTGCCAGGGTTTTTAGAGATGAAGATTTAAGAAAAGATAGACAACCAGAATTCACTCAATTAGATATTGAAATGTCATTTGGAAGTCAAGAAGAAATATTTAAATTATGTGAAGAAATGTGA", "aa": "MQLIFQNVSDPLLTKESCIYVKGTVQKRLEVNPALKTGEIEVIVSDYKIFNSAKQIPFEINNSKSTNDANEDIRLEYRFLDLREERMLNNLRLRHKFLLLVRNFFDKQGYIEVETPILGKSTPEGARDYLVPTRRKGRFFALPQSPQLFKQLLMAAGFEKYFQIARVFRDEDLRKDRQPEFTQLDIEMSFGSQEEIFKLCEEM", "aa_hexdigest": "56df0164e538cf164b07c9a2d475a141", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_G9YIR7", "query_cov": 0.9310344827586207, "subject_cov": 0.8217391304347826, "identity": 0.556, "score": 193.0, "evalue": 7.66e-59, "valid": false, "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.-" ], "uniref50_id": "UniRef50_A0A951N4R3", "go_ids": [ "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 136, "aa_cov": 0.6600985221674877, "hmm_cov": 1.0, "evalue": 1.2248726575429562e-60, "score": 200.42628479003906, "start": 70, "stop": 203 } ], "seq_stats": { "molecular_weight": 23943.272099999984, "isoelectric_point": 6.65228786468506 }, "id": "AHCKFKLKGB_414", "locus": "GCA013367975_00389" }, { "type": "cds", "sequence": "contig_1", "start": 220052, "stop": 220372, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGGTACAAAATTGAAACTCCATTTCCAAAAATGGACTTTTTCTATTCAATGGCTCATTATGGAAATGATAAACCAGATACAAGATTTGGATTTTTAATTGAAGATTATTCAAAACAATTTGCAGCCAAATATAATAAGAAATTTGTAAAAGCTATTGTTTTTGATAGAAATATAAATGAACATAAACAAATAATTAATGAAACTTTTAAAAAGAATAATGGACAAATATTAGACATATTCGACTTAAAAGAAGCAAACCAAAATGAATTTCAATGTCTTTTAGATAAGGCAAAAGAAGATAATATTTCAACCCCATGA", "aa": "MGYKIETPFPKMDFFYSMAHYGNDKPDTRFGFLIEDYSKQFAAKYNKKFVKAIVFDRNINEHKQIINETFKKNNGQILDIFDLKEANQNEFQCLLDKAKEDNISTP", "aa_hexdigest": "369ba5b3bcb30183525d491ce220a7a8", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12543.092099999985, "isoelectric_point": 6.724530220031738 }, "id": "AHCKFKLKGB_415", "locus": "GCA013367975_00390" }, { "type": "cds", "sequence": "contig_1", "start": 220505, "stop": 220975, "strand": "+", "frame": 2, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FB0CEA" ], "nt": "ATGTTTGAATATGACAGAGAAACAAAAACATATTCACCTGCTCACCATGCGTTTACACAATTTGAAGAATCTACAATGAAATATTTAGAAACTGGAGAGCTTGAAAAAGTTAGAGCTAAATCATATGATTTAGTGTTAAATGGATTTGAATTAGGATCTGGTTCTATAAGAATTTATGATCCAAAGATTCAAAAGAAAATGTTCGACGTAATTAATCTAAGTGAAAAACAACAACAAGATCGTTTCGGATTCTTTTTAAAAGCATTTGAATACGGTTTACCTCCTCACAATGGTATTGCATTTGGAGTTGAAAGAATTTTAATGATTTTAACCAAATCAGAATCAATTAGAGATGTAATTGCATTTCCTAAAAATGCTAAAGGCATTGACTTGTTGACATCAGCTCCTAGCGATGTGACAAAAGAACAATTAGATGAATATGGATTGGAACTTAAAATTAATAAAAAATAA", "aa": "MFEYDRETKTYSPAHHAFTQFEESTMKYLETGELEKVRAKSYDLVLNGFELGSGSIRIYDPKIQKKMFDVINLSEKQQQDRFGFFLKAFEYGLPPHNGIAFGVERILMILTKSESIRDVIAFPKNAKGIDLLTSAPSDVTKEQLDEYGLELKINKK", "aa_hexdigest": "f007a3977b2685b4435b15c340846545", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021FB0CEA", "query_cov": 0.9743589743589743, "subject_cov": 0.8837209302325582, "identity": 0.5660000000000001, "score": 176.0, "evalue": 1.44e-53, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI0021FB0CEA" }, "pscc": { "uniref50_id": "UniRef50_UPI0021FB0CEA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FB0CEA" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "AHCKFKLKGB_416", "locus": "GCA013367975_00391" }, { "type": "cds", "sequence": "contig_1", "start": 221067, "stop": 221246, "strand": "+", "frame": 3, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0015136", "GO:0015739", "GO:0016020", "SO:0001217", "UniRef:UniRef50_I5D5Q9", "UniRef:UniRef90_D1J7X6" ], "nt": "ATGCATGCTGCTTCAATAGCCTTAACAGTTATATTAGTAATAATTTCATTTGCGATAATTGGAATTTCATTTATTATGGCTCCTGATTCAAATAGTTTTAGTGGAGCTTTAGTTGGTTCAAATGACCTAGACTTGTTCAAAGTATCAAAAGAAAGAGGAATAAAAAAAGTATTAAAGTGA", "aa": "MHAASIALTVILVIISFAIIGISFIMAPDSNSFSGALVGSNDLDLFKVSKERGIKKVLK", "aa_hexdigest": "287b3e133ec6143731f4c8470f1a19f0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "secG" ], "truncated": "3-prime", "pseudogene": { "start": 221067, "stop": 221318, "observations": { "mutation": [ 221244 ], "stop": [ 221244 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X6", "query_cov": 1.0, "subject_cov": 0.7108433734939759, "identity": 1.0, "score": 105.0, "evalue": 2.03e-28, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MHAASIALTVILVIISFAIIGISFIMAPDSNSFSGALVGSNDLDLFKVSKERGIKKVLKWSMITLGILLFIFLIALRVVIQNG" }, "paralog": false, "identity": 0.9879518072289156, "score": 151.0, "evalue": 2.89e-49, "description": "Internal stop codon at 221244. Nonsense mutation around 221244.", "psc": { "uniref90_id": "UniRef90_D1J7X6", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_I5D5Q9", "go_ids": [ "GO:0015136", "GO:0015739", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X6", "UniRef:UniRef50_I5D5Q9", "GO:0015136", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_I5D5Q9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D5Q9" ], "product": "Protein-export membrane protein SecG" } }, "id": "AHCKFKLKGB_417", "locus": "GCA013367975_00392" }, { "type": "cds", "sequence": "contig_1", "start": 221334, "stop": 222224, "strand": "+", "frame": 3, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_B3PMK9", "UniRef:UniRef90_D1J7X7" ], "nt": "ATGACATATATAAAAAATTTTAAAAATAAGAATAACAACCCTAAAATTAATGAAGAACAAGTATTAAAATGCATAAGAGAAAGTGATGGAATTACTTTTATTGAAATTGCAAAAAAATTAAGAATTCCTGCTTTTTTGAATTTTGAATTATCAGAAATATTAAATAACTATTTATCTAAAAAATTAATTGATACGAAAAGCGATGAAAAATACATCGCTATTTATTTTCTAACTAAAGTACAAAAGGAAATATCAATAACTGCCAAAAGATTAGGATTTATTGATTTTGAAATGCAAGAAGAATCTCAAACTAAAGTATTTAGTGCCTTTCTTGCACCTTTTCAATTAAAAGCGGTATTAGACAAGGATTTATTGCTTGCAAATATTTATTATTATTTTGAAAATGGCATAAAAAAATATAAAGGCGATATTATTCAAATTTTAAATCACGCAAGAAAAACTATTGTTGGGACTATTGAAAAGAGCGCAAATAAACTGTTTTTTAATGCTTATGATGAAAAAGATAGGGCTAAATTTGAAATTATTAATGAAAAAGAATGCCCAGATATAACAAAATTGAAAAATTCATTGGTATCTTGCGAGATTTTAAAACCAAATAATAAAAAAGTTGCTATTGCATATAAAAATATAATTGGTAATATTAATGATAAAGAACATGTGATTAAAAAAATCATTGCCCAAAATGATGTAAATGTCAATTTTCCACAAAATTTATTAGAATATGTTTCCTCAATACCTTCTTTTGTTTCACAAGAAGAAATTAATTCAAGAATAAATTTAATAGATTTAATGACAGTTACAATTGACGGTCTTGACACTAAAGATTTTGATGACGCCATTTCGTGTTATAAAAATGAAAAGAATAATTGA", "aa": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYFENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNN", "aa_hexdigest": "3c8347faa9420ca11f255c5a0bdcbfd4", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "3-prime", "pseudogene": { "start": 221334, "stop": 222527, "observations": { "mutation": [ 222222 ], "stop": [ 222222 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X7", "query_cov": 1.0, "subject_cov": 0.41283124128312415, "identity": 0.997, "score": 570.0, "evalue": 8.22e-199, "gene_start": 1, "gene_end": 296, "reference_start": 1, "reference_end": 296, "reference_sequence": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNNWKLFIHIADVSYYVKENDLIDNEALLRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA" }, "paralog": false, "identity": 0.9949622166246851, "score": 780.0, "evalue": 6.1e-284, "description": "Internal stop codon at 222222. Nonsense mutation around 222222.", "psc": { "uniref90_id": "UniRef90_D1J7X7", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_B3PMK9", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X7", "EC:3.1.13.1", "UniRef:UniRef50_B3PMK9", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK9" ], "product": "Ribonuclease R" } }, "id": "AHCKFKLKGB_418", "locus": "GCA013367975_00393" }, { "type": "cds", "sequence": "contig_1", "start": 222297, "stop": 223487, "strand": "+", "frame": 3, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_B3PMK9", "UniRef:UniRef90_D1J7X7" ], "nt": "TTGCGTGGCACAAGCATTTACTTACCTGATAGGGTTATACCAATGTTGCCGGTTGAACTTTCTAACGGAATTTGTTCATTAAACCCAAATGAAGTCAGAGCTTGTATAACATTGGAATTAGAAATAGACAAGCAAGGGAACAATATATCATATAAAATTTATCCTAGTGTTATAAAATCAAATTATAGATTGACTTATAATGAAGTTAATGATTTTTATAGCAATATAAAAAGTGTTCCTGATGATGTTGCAAATATGCTGTTGCATGCACGAGATCTTGCAAAAATTATTAGAAGTAAAAAAATATCCGAAGGTTATGTTGACTTTGAAATTCAAGAATCAAAAGTTATAATGGATGGAACAAAGGTTGTTGATATCAAGGTGAAAGAAGAAGGCGAAAGCGAAAAATTAATTGAAGACTTCATGGTTCGGGCAAACGAAACCGTTGCTCAAATGATGACTAATTTAAAAATTCCTTCAATTTATAGAATTCACGAAAAACCTTCACCTGAAAAACTAACTTTATTGCAAGAATTATTCAATTTTTGTGGATTTAAGGATGTAAAAGTTCCTTATGATGGAGAACCAAAATCATTTGGAGAAATGATTGCAAAATTAAAAGAACATAAATTTGATGATTTTATTAAAATGGCATTATTAAGAACAATGCAAAAAGCAATATATTCATCAAATAATATTGGCCATTTCGGTTTAGCTTCAAAAGCTTACTCACATTTTACTAGTCCAATTAGAAGATATCCAGACTTATTGCTACATAGATTGATAAGAACTTATATTTTTGAAAAGAAAGAAATAACACCTGATTTATCAGAAACGTTAAAACAAAAAATAAGTCAAATTGCTTTAATGAATAGCGAATCTGAAAAAAATGCAATGGTTGTCGAACGAAGTGTTGTTGATGTTAGAAAATCAGAATTCTTTGAAAATTTAATTAATAAGCAATTTTTTGCAACATTAGTATCAATAGAAAAATTTGGAATGTTTTTTAATATTGATGAATACCAAACAAGTGTTTTAGTTCGTTTTGAAAATCTTGCGATTCCAACTAATAAAGACAACGATTTTCAAGCTTCATCTAAAAATAAAATCTATAAAGCAGGCAATAAATATTTAATTACTATCACTTCGATAGATCATGAAAAAGGAAATGTAAATGCAACATTGGCTTAG", "aa": "MRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA", "aa_hexdigest": "52a4d609eb5fb8047bbdc1b03fd17a15", "start_type": "TTG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "5-prime", "pseudogene": { "start": 221997, "stop": 223487, "observations": { "mutation": [ 222222 ], "stop": [ 222222 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X7", "query_cov": 1.0, "subject_cov": 0.5523012552301255, "identity": 0.997, "score": 768.0, "evalue": 3.19e-275, "gene_start": 1, "gene_end": 396, "reference_start": 322, "reference_end": 717, "reference_sequence": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNNWKLFIHIADVSYYVKENDLIDNEALLRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA" }, "paralog": false, "identity": 0.9979838709677419, "score": 972.0, "evalue": 0.0, "description": "Internal stop codon at 222222. Nonsense mutation around 222222.", "psc": { "uniref90_id": "UniRef90_D1J7X7", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_B3PMK9", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X7", "EC:3.1.13.1", "UniRef:UniRef50_B3PMK9", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK9" ], "product": "Ribonuclease R" } }, "id": "AHCKFKLKGB_419", "locus": "GCA013367975_00394" }, { "type": "tmRNA", "sequence": "contig_1", "start": 223988, "stop": 224358, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 224071, "stop": 224148, "aa": "AEEKQNKQSFVLNQMMSSNPVFAY", "nt": "GCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAA" }, "nt": "GGGGGCGTACAGGCTTCGACATACATGCAAGTTATTAAGTTGCAGTAGTTTGGCAAACTATAATGCTACTAGGCTTTTTAAGCGCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAAATTCTGGTAGGCCAATGAATAGCTCGGTTCATTGTTAGCCTTTGGAGAGCTTAGCAATTAAAGTGGCGTTTTAATTGCAAAATTATGCCAATGGTTATTATGAAGGTTGTTAATTTGATAATAATATAACCAAACATAAAATTAACTAAACTGTAGATACTTAATAACAAACATGTGTGGACCGGGGTTCGATTCCCCGCGCTTCCACCA", "id": "AHCKFKLKGB_34", "locus": "GCA013367975_00395" }, { "type": "cds", "sequence": "contig_1", "start": 224568, "stop": 224750, "strand": "+", "frame": 3, "gene": null, "product": "DUF2188 domain-containing protein", "db_xrefs": [ "RefSeq:WP_020002640.1", "SO:0001217", "UniParc:UPI0002F57F90", "UniRef:UniRef100_A0A2K9YST0", "UniRef:UniRef50_B3PMJ9", "UniRef:UniRef90_A0A2K9YST0" ], "nt": "ATGGCGGTCAGATTAGTAGTAAAGCATGAAGATGGTTGGGCAGTCAAAAATCCAAAAGGAAAAAGAGCATTGAGAGTATTTAAGACTCAAAAAGAAGCTGTTCAATATGCAAAAACTTTGCAAGATACTTCATCTGTAAATGTTCAGTCTAAAGCTGGAAAGTTTAGAAAGTTCACAAGCTAA", "aa": "MAVRLVVKHEDGWAVKNPKGKRALRVFKTQKEAVQYAKTLQDTSSVNVQSKAGKFRKFTS", "aa_hexdigest": "9af3d45b90630dd907ce1f2a199efb17", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0002F57F90", "ncbi_nrp_id": "WP_020002640.1", "uniref100_id": "UniRef100_A0A2K9YST0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F57F90", "RefSeq:WP_020002640.1", "UniRef:UniRef100_A0A2K9YST0" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YST0", "uniref90_id": "UniRef90_A0A2K9YST0" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YST0", "product": "DUF2188 domain-containing protein", "uniref50_id": "UniRef50_B3PMJ9" }, "pscc": { "uniref50_id": "UniRef50_B3PMJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMJ9" ], "product": "DUF2188 domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_420", "locus": "GCA013367975_00396" }, { "type": "cds", "sequence": "contig_1", "start": 224707, "stop": 225630, "strand": "+", "frame": 1, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "COG:COG0190", "COG:H", "SO:0001217", "UniRef:UniRef50_D1J7X9", "UniRef:UniRef90_D1J7X9" ], "nt": "ATGTTCAGTCTAAAGCTGGAAAGTTTAGAAAGTTCACAAGCTAAAATGGCAATATTATTAAATGGTAAAGCAATCTCTTTAGAAATTAAAGAAGATTTAAAAAAAGCTATTGAAAAAATGCCTCAAGAAATTAGACCTACTTTAGGAATTTTACAAGTAGGCGATTTAAGTGAGTCAAATATTTATGTAAAAAATAAACTTAAATATGCTTCTGACATAGGATTAAATACAAAACTAATAAAATTGCAACAAAATGCAAGTAAATTGCAAATTTCTGAAGCAATAGAATTATTAAATAAACAAAGCACCGGATTTATAGTTCAAATTCCAATGCAAACTAATCAAATTATAGATAGTCAAGAAATATTGGATTTAATAAGCATAGATCACGATATTGATGGTCTAACCTCTTTTAATCTTGATTTAAAAAATCCTAATAATTATTTTTTATCAGCTACTGCTAGTGGAATTTTTTTGCTTTTAAACAAATACAATATTCTTTATAAAAATGCAAAAATTGGAGTTATTGGTCAAAGCAAAATTGTCGGGTACCCATTGGCAAATTTCTTAGTTTTAAAAGGATATAACGTATTTAGATACAATAAAGATACTCCTAAAAATAATATTAAAGACTTGGATATAATAATTGTTGCAACCGGCGCAAAAGATCCAATTACATACGACCAAGTAAAACCTGGGGCGGTTATTATTGATGTTGGAATACACAGAGATAGCAATAATAAAATTTCAGGGGATTTAAATTTTAATGAATTTGCTAATGTTGCTTCATTTATAACCCCTGTTCCTGGCGGAGTTGGTCCAATGACTATCTCGGCATTGATCATTAATCTTTTAAAGGCAAGTTCTTTACAAAATCCAATTATTTTACCATATCTTAAATTTTTGAAATCATATTTTAATTAA", "aa": "MFSLKLESLESSQAKMAILLNGKAISLEIKEDLKKAIEKMPQEIRPTLGILQVGDLSESNIYVKNKLKYASDIGLNTKLIKLQQNASKLQISEAIELLNKQSTGFIVQIPMQTNQIIDSQEILDLISIDHDIDGLTSFNLDLKNPNNYFLSATASGIFLLLNKYNILYKNAKIGVIGQSKIVGYPLANFLVLKGYNVFRYNKDTPKNNIKDLDIIIVATGAKDPITYDQVKPGAVIIDVGIHRDSNNKISGDLNFNEFANVASFITPVPGGVGPMTISALIINLLKASSLQNPIILPYLKFLKSYFN", "aa_hexdigest": "3412379da61bb2e7c8e0d79e3f773348", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7X9", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9740000000000001, "score": 574.0, "evalue": 1.66e-206, "valid": true, "gene": "FolD", "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_D1J7X9", "cog_id": "COG0190", "cog_category": "H" }, "pscc": { "uniref50_id": "UniRef50_D1J7X9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7X9" ], "product": "Bifunctional protein FolD" }, "genes": [ "folD" ], "id": "AHCKFKLKGB_421", "locus": "GCA013367975_00397" }, { "type": "cds", "sequence": "contig_1", "start": 225730, "stop": 225867, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATAGGTGTTAGTGGTATGATTGCCGCCGGTAAAAGTTCGTTAGCTGAAAAACTTCACCGTTATTTTGAAGGTTCGCTTATGCTTCATGAATTTGAAGAAGATGATGAGGTATTTAATACATTTTTAAAATGA", "aa": "MIIGVSGMIAAGKSSLAEKLHRYFEGSLMLHEFEEDDEVFNTFLK", "aa_hexdigest": "0bfc1de864416f9cb21613380c6e5897", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5091.789099999999, "isoelectric_point": 4.8198556900024405 }, "id": "AHCKFKLKGB_422", "locus": "GCA013367975_00398" }, { "type": "cds", "sequence": "contig_1", "start": 225991, "stop": 226209, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01712.25" ], "nt": "ATGATTTTTTTGGATAGATTTTCAATAGAACATTATATTTTTGCAAAATTAATTTTATCTAAAAAAGGTAAAAGATATTTAGATGCATATGATGCTTTGTTTGAAAAATTAATTAGTAAAGATGAATTGCCTGATTTTGCAATCTTTTTAGATATTAATTTTGAAACATTTAAAAAGAGAATATTTACACGCGGAAGACAAAGTGAAGTAGATAACTGA", "aa": "MIFLDRFSIEHYIFAKLILSKKGKRYLDAYDALFEKLISKDELPDFAIFLDINFETFKKRIFTRGRQSEVDN", "aa_hexdigest": "579e8b1a95c98a26c99315edf116751f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01712.25", "name": "dNK", "length": 62, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 9.511032884737158e-08, "score": 27.327556610107422, "start": 10, "stop": 69 } ], "seq_stats": { "molecular_weight": 8669.956499999993, "isoelectric_point": 7.985392570495606 }, "id": "AHCKFKLKGB_423", "locus": "GCA013367975_00399" }, { "type": "cds", "sequence": "contig_1", "start": 226521, "stop": 226868, "strand": "+", "frame": 3, "gene": null, "product": "Hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PE13" ], "nt": "TTGCCATTTTTGGCGCAGTTAATTAAAGACGTAACTGTAGATTTTGTTTTGGATTGCATGATAACTAGCTCATATGATGGAAAAGATAGATCTTCGGGAAATGTCAAAATAACATTGGATTTACAAAATAATATTGCAGATAAAGATGTATTAATTGTTGAAGATATAGTAGATAGTGCTAGAACTATGACCAAAGTTGTTTCACTGCTAAAATCAAGAAATCCTAAATCATTAAAAGTTCTTACTTTACTTGATAAACCATCTGGCAGATTAGTAGAATTTAAACAAGATAACTTTGGTTTTGAAATCGGCAAAGATGACTTTGTTGTTGGCTTTGGCTTCGATTGA", "aa": "MPFLAQLIKDVTVDFVLDCMITSSYDGKDRSSGNVKITLDLQNNIADKDVLIVEDIVDSARTMTKVVSLLKSRNPKSLKVLTLLDKPSGRLVEFKQDNFGFEIGKDDFVVGFGFD", "aa_hexdigest": "a53522b5963d9e722b06c02c046cf37a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0PE13", "query_cov": 0.991304347826087, "subject_cov": 0.8085106382978723, "identity": 0.526, "score": 113.0, "evalue": 4.58e-30, "valid": false, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_A0A3B0PE13", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PE13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PE13" ], "product": "Hypoxanthine phosphoribosyltransferase" }, "genes": [], "id": "AHCKFKLKGB_424", "locus": "GCA013367975_00400" }, { "type": "cds", "sequence": "contig_1", "start": 226954, "stop": 227310, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y2", "UniRef:UniRef90_D1J7Y2" ], "nt": "ATGATTCTTAAGTTACAAAATTGGCAAATAACAGAAACTTCAAATCAAGATAGTGGCACTAGCATACCTTTAGCTATTGGCTCTGCGTTTGTGGCATTTATTTTAATAGTTGCTGTAGTTATTTTTATAATAAAAAAAATAATAAATAAGCAATCAAAAACAGAAGATAATTCAACTAATAAAGAATTAAATAAAGACAATAAAAATGACGAATATAATAAAAAAATTACATTGCCTTTTTTTAATTCTGAAGACCTAAAAAGAATAGATAAATTAAATTCCTTAAAAATTGAAAAACAAATTGCTTATTTATTTGACTCGCAAAAATTTTTTAAAGAAAATTTAAATAATTTATGA", "aa": "MILKLQNWQITETSNQDSGTSIPLAIGSAFVAFILIVAVVIFIIKKIINKQSKTEDNSTNKELNKDNKNDEYNKKITLPFFNSEDLKRIDKLNSLKIEKQIAYLFDSQKFFKENLNNL", "aa_hexdigest": "61a3475ece98c1607ce254f458299887", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 226897, "stop": 227613, "observations": { "mutation": [ 226906, 227308, 227428 ], "stop": [ 226906, 227308, 227428 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Y2", "query_cov": 1.0, "subject_cov": 0.4229390681003584, "identity": 0.9490000000000001, "score": 208.0, "evalue": 1.57e-65, "gene_start": 1, "gene_end": 118, "reference_start": 20, "reference_end": 137, "reference_sequence": "MVYWQIKKLINTKNTNNFSIILKLQNWQITETSNQDSSTSITLAIGSAFVAFILIAAVVIFIIKKIINKQSKTRDNSTNKELNKDNKNDEYNKKITLPFFNSEDLKRIDKLNSLKIEKQIAYLFDPQKFFKENLNNLWVKKYYEIIKKFKLEKSPTSKFNKLKINKYYKNYLNEVGQWNYCRHHIDEIKISGAIYSAHPQTKEYYKTGDVILVTYEQHLFLHYLIVMAKTTMPNHGMIVGLKFHFNDTKKCIEYWDKVIQKMCEKYLVKYDKNWSENLT" }, "paralog": false, "identity": 0.9495798319327731, "score": 450.0, "evalue": 1.89e-163, "description": "Internal stop codon at 226906, 227308, 227428. Nonsense mutation around 226906, 227308, 227428.", "psc": { "uniref90_id": "UniRef90_D1J7Y2", "uniref50_id": "UniRef50_D1J7Y2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Y2", "UniRef:UniRef50_D1J7Y2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Y2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13627.542699999976, "isoelectric_point": 9.09856815338135 }, "id": "AHCKFKLKGB_425", "locus": "GCA013367975_00401" }, { "type": "cds", "sequence": "contig_1", "start": 228453, "stop": 229250, "strand": "+", "frame": 3, "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "RefSeq:WP_175933436.1", "SO:0001217", "UniParc:UPI001588AFE3", "UniRef:UniRef100_UPI001588AFE3", "UniRef:UniRef50_Q98QH5", "UniRef:UniRef90_D1J7Y4" ], "nt": "ATGATTGAAATTAAAAAAATATCTTATAAATATCCAGGTTCATCGAAGCTAGCGTTAGAAAATATTGATCTTAAAATTGAAGAAGGCCAATATATTGCTATTTTAGGTCATAACGGTTCGGGAAAAAGCACATTTTCTAAACTTTTATCTGCAATTTATAAACCAACATCAGGAAAAATAATAATTGATGGATTAGAAATTAATTCAGAAAATTTACTAGAAATTAGAAAAAAAATTGGAATCGTATTTCAAAATCCTGATAACCAATTTATTGGTTCAACTGTTGAAGATGATATTGCTTTTGGTCTTGAAAATAAAATGTTGAAACCTGAAGAAATGAAAACAATTATTTATAAATATGCTCAAGAAGTCGGAATGCTAGATCAGTTAGATAGAGAACCACAAAATCTATCTGGTGGTCAAAAACAACGCGTTGCAATTGCTTCAACATTAGCCCTAGATCCTAAAATAATTATTTTCGATGAAATTACTTCAATGCTAGATCCTAAAGGGCGTAATGATATTTATGAAATAATTCATAATTTACACAAAAAAACTAATAAAACATTAATTTCTATCACACATGATATGGATGAAGCACTACTAGCAGATAAATTAATAGTATTTGCTGGCGGTCATGTTATTGCTCAAGGTAAACCAATAGAAATCCTAAATAACAAAGAAATTATTGAAATTGCAAAAATAGATTCTCCATTTATCTATAAACTAAGTAGTCTCTTAAATGATATTGAGCCAACATATAATAGAGAGGAATTAATTAAAGAAATATGCAAATAA", "aa": "MIEIKKISYKYPGSSKLALENIDLKIEEGQYIAILGHNGSGKSTFSKLLSAIYKPTSGKIIIDGLEINSENLLEIRKKIGIVFQNPDNQFIGSTVEDDIAFGLENKMLKPEEMKTIIYKYAQEVGMLDQLDREPQNLSGGQKQRVAIASTLALDPKIIIFDEITSMLDPKGRNDIYEIIHNLHKKTNKTLISITHDMDEALLADKLIVFAGGHVIAQGKPIEILNNKEIIEIAKIDSPFIYKLSSLLNDIEPTYNREELIKEICK", "aa_hexdigest": "3bc49e8373d42e35756742178f546d17", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001588AFE3", "ncbi_nrp_id": "WP_175933436.1", "uniref100_id": "UniRef100_UPI001588AFE3", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588AFE3", "RefSeq:WP_175933436.1", "UniRef:UniRef100_UPI001588AFE3" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588AFE3", "uniref90_id": "UniRef90_D1J7Y4" }, "psc": { "uniref90_id": "UniRef90_D1J7Y4", "gene": "EcfA2", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q98QH5", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QH5" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [ "ecfA2" ], "id": "AHCKFKLKGB_426", "locus": "GCA013367975_00402" }, { "type": "cds", "sequence": "contig_1", "start": 229241, "stop": 229540, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZL6", "UniRef:UniRef90_UPI001E584429" ], "nt": "ATGCAAATAAAAGCTTCTGAAATTACCAAAGAATACGACAAAGGATTGCCCACTTATATAAAAGTTATTGATAATGTAAGTTTAGAAATATCAGAAGGAGAAGCAATATCCATTATTGGCCCAACCGGTTCAGGGAAAACAACTCTTATTGAACATTTTAATGGTTTAATTATTCCTTCAACTGGAAAAATTATTTTTAATGAGTTGCCAATAAAAATTAAAACTCCAAAACCTAAAAAACCTAACTTAAAAAAAATTAAACATGATTCAAATGAATATGAAATTGTTCTTGAAAAATGA", "aa": "MQIKASEITKEYDKGLPTYIKVIDNVSLEISEGEAISIIGPTGSGKTTLIEHFNGLIIPSTGKIIFNELPIKIKTPKPKKPNLKKIKHDSNEYEIVLEK", "aa_hexdigest": "fffb3cabe1c8f86cb2544f66da902a73", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI001E584429", "query_cov": 0.8484848484848485, "subject_cov": 0.9545454545454546, "identity": 0.976, "score": 162.0, "evalue": 1.63e-50, "valid": true, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A448ZZL6" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZL6" ], "product": "Cobalt ABC transporter ATP-binding protein" }, "genes": [], "id": "AHCKFKLKGB_427", "locus": "GCA013367975_00403" }, { "type": "cds", "sequence": "contig_1", "start": 229664, "stop": 230038, "strand": "+", "frame": 2, "gene": null, "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "SO:0001217", "UniRef:UniRef50_Q4A5A4", "UniRef:UniRef90_UPI0019672E9D" ], "nt": "GTGGGAGTTGTATTTCAATTTGCTGAATATCAACTTTTTGAATCAACCATTGAAAAAGATATAATTTTTGGACCTATTTCAATGGGTGTAAGTAAAGATGAGGCTAAAAAATTAGCACTTAAATATATTGAAATGGTTGGATTGCCTATTGACTATTTGCAAAGAAGTCCATTTAATTTATCAGGCGGTCAAAAACGTAGAGTTGCGTTAGCTGGTATATTAGCAATGGAACCAAAATTTCTAGTTTTAGATGAACCAACTGCCGGGCTTGATCCGCAAGGTGTCGAAGAAATGCTAAACTTATTTCATAATCTCTATTTAAATGGAAAAACGATTATTATTGTAACACATGACCTTGATAATGCCTTAAAATGA", "aa": "MGVVFQFAEYQLFESTIEKDIIFGPISMGVSKDEAKKLALKYIEMVGLPIDYLQRSPFNLSGGQKRRVALAGILAMEPKFLVLDEPTAGLDPQGVEEMLNLFHNLYLNGKTIIIVTHDLDNALK", "aa_hexdigest": "9f34442d53614fac91ff9a4b5cd8ea2d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 229364, "stop": 230266, "observations": { "mutation": [ 229538, 229559, 229592, 230036 ], "stop": [ 229538, 229559, 229592, 230036 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019672E9D", "query_cov": 1.0080645161290323, "subject_cov": 0.40584415584415584, "identity": 0.8240000000000001, "score": 202.0, "evalue": 6.19e-63, "gene_start": 1, "gene_end": 124, "reference_start": 109, "reference_end": 233, "reference_sequence": "MQIKVEKISKEFDKKLPTHIQVLKDISVDIKEGEIISIIGHTGSGKTTFIEHLNALIIPDSGKITFSNVDVNKKQWYQSKNKLQLVKMDIVVEKTKRKIKNIKSLRKEVGVVFQFAEYQLFESTIEKDIIFGPVSMGVPVEEAKKLAKKYIKMVGLPESYLQRSPFNLSGGQKRRVALAGILAMQPKYLILDEPTAGLDPAGVEQMLQLFANLNKEEGKTVIIVTHDLDNALRWTKRCILFKNGEIIRDGETYEVLNDEQCLIENKLRATQLLTFVNKLKARGVKIGKVTDINQLAEQINNWKQNKGV" }, "paralog": false, "identity": 0.5980066445182725, "score": 328.0, "evalue": 1.39e-114, "description": "Internal stop codon at 229538, 229559, 229592, 230036. Nonsense mutation around 229538, 229559, 229592, 230036.", "psc": { "uniref90_id": "UniRef90_UPI0019672E9D", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q4A5A4", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019672E9D", "UniRef:UniRef50_Q4A5A4", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5A4" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" } }, "id": "AHCKFKLKGB_428", "locus": "GCA013367975_00404" }, { "type": "cds", "sequence": "contig_1", "start": 230102, "stop": 230278, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGATGACAATTTCTTAATAGAAAATAATTTAATTCCTACTAGATTATTATCGTTTGTTAATGAATTAAAAAAGAATAATGTAGATGTAGGTAAGGTAACAAGTGTTGAAGATTTAGCTGAAAAAATTAATTATTACTTAACCAAGAAGCGCAAGGGAGGAACAAATGAATAA", "aa": "MSDDNFLIENNLIPTRLLSFVNELKKNNVDVGKVTSVEDLAEKINYYLTKKRKGGTNE", "aa_hexdigest": "2307ad223dc409822875b731fcbc831b", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6630.448700000003, "isoelectric_point": 6.218833732604979 }, "id": "AHCKFKLKGB_429", "locus": "GCA013367975_00405" }, { "type": "cds", "sequence": "contig_1", "start": 230271, "stop": 230438, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAACAATTATTGGTAAATATTTAAATTACGATACATTTATTCACAAAATTGATCCTCGCTTCAAATTTTTAACAAATGTTTTATTAATAATATTGCTTTTTATGGCTGATCACTTTATAACTTTGGGAATTTTAATTTTCTCAATAATGATTTTTTATTGA", "aa": "MNKTIIGKYLNYDTFIHKIDPRFKFLTNVLLIILLFMADHFITLGILIFSIMIFY", "aa_hexdigest": "4d86250178570e8cd34577cd44f37af2", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6584.976999999995, "isoelectric_point": 9.224603843688964 }, "id": "AHCKFKLKGB_430", "locus": "GCA013367975_00406" }, { "type": "cds", "sequence": "contig_1", "start": 230693, "stop": 230875, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACGTAATGATATTGAAATAATCATTGTTATAATTTCTGTAGGAATTTTAACTAACTTTAAAGGGATTAAAATATCATTAATTGCTCTAGTTAGTAAAACTGGTTTTGTTGAAACTGTTAAAATTGTTGTTGTTAAAATAACACCATATATTCTTAAAAATATTGATAACGTTCTATAA", "aa": "MKRNDIEIIIVIISVGILTNFKGIKISLIALVSKTGFVETVKIVVVKITPYILKNIDNVL", "aa_hexdigest": "79326af5fa4742a7b566d38cc92c8a5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6652.105000000001, "isoelectric_point": 9.777162361145017 }, "id": "AHCKFKLKGB_431", "locus": "GCA013367975_00407" }, { "type": "cds", "sequence": "contig_1", "start": 230907, "stop": 231104, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCACAAGCAAGTAGAGGTATCGATTTTAAAAACGGAAATTTAAAGGATAAGACTAAATCCTTTATTGTTTTGATTGTTCCACTATTTGTTTCTTCATTTTCAAAGGCGAATGATTTGGCTGACGCAATGACATCAAGAGGTTATGAACCTTATGCTAAAAGATCTCACTATAGAGTGTTGACACCTTGTTGA", "aa": "MKAQASRGIDFKNGNLKDKTKSFIVLIVPLFVSSFSKANDLADAMTSRGYEPYAKRSHYRVLTPC", "aa_hexdigest": "10873f30714aa4d79dbb0e5dc19d092a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7284.358300000002, "isoelectric_point": 9.841888618469238 }, "id": "AHCKFKLKGB_432", "locus": "GCA013367975_00408" }, { "type": "cds", "sequence": "contig_1", "start": 231442, "stop": 232914, "strand": "+", "frame": 1, "gene": null, "product": "DNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5IJW6" ], "nt": "TTGGCTTCCGATCTATTTAAAAAAGTAAAACATAAACGTCCAATGCTATCTTTAAATAAAGCGTATTCGTTAGATGAAATTCAAAAATTTATTGATAATATAAAGAAAATTACGTCTGATTTTTCTTTTTTTATTGAGCCTAAAATTGATGGTCTTTCAATATCTATTATTTACGAAGATGGCAAATTAGTTAGAGCAATAACAAGAGGAGATGGAATAACTGGCGAAGATGTTACGGCAAATGTTATTCAAATGTCTTGCATTCCTAAAACTATTAAATATAAGAAATCATTAGAGGTAAGAGGGGAAGTTTATTTAGCAATATCTGATTTTGAAAAATTAAATTTAAAATTAGGACAAGAAAATAAAAATAAACTTGCAAATCCTAGAAATGCAGCAGCAGGCACATTAAGGCAATTAAATTCTCAAATTATTAAAGAACGCAATTTGTCAGCATTTTTGTATTATGTTGTGGACCCTTTGCAACATAATATTAAAACAATGCAAGAAGAACATGAGTTTTTAAGAACTTATGGTTTTCCTGTTACTCCTGAAGTTCAACACGCTAACACATTACAAGATATTGAAGAATATATTAGAAACTTCAAGAATCAGAAACTATTAATAGATTACGAAACAGATGGAGTTGTTATAAAGTTGAACGAAGTTCAATATTTTGACAAGTTAGGATCAACGGCTAAGTTTCCCCATTATGCAATTGCATTTAAATATGAACCTAATGTTGAAACAACAACAATAAAAAGAATATACGCAACAGTAGGAAGAACAGGATTAGTTACGTATAATGCAGAATTAGAACCTGTAGAAATTTCTGGTAGTCTCGTAAGCTTTGCAACATTGAATAATTTTCAATATATAAAAGATTTAAATCTAAATGAAAATGATTTAGTATATATTAAAAAAGCTGGAGAGATTATTCCTTGTGTAATTGGCTTAATTTCTGGCAAAAAAGAAATTGATCATTTTCAAAAAGTAGAATTCTGCCCATATTGTCACAATAAACTTGTCGATTCAGAAACGGGATTGGAGCAATTTTGCTCAAATGATCAATGTCCTGAAATACAAAGAAGAAAATTGATTCATTTTTGTTCAAAAGACGCTTTAGATATTAATACTATGGGTGAGAGAAATATTGATCTATTTTTAAAAAATTCTTTAATTTCCAACCCTATTGATATTTTTAATTTAAAGAACAAAAAACAAGATTTATTAAAATTAAATAGAATGGGTGAAAAATCCATTGAAAATATTTTGATTTCCATTGATGAATGTAAAAATAAACCTTTATATAAAATGATTTCTGCCCTTTCAATCCCTCTAATTGGTGTAAAATTAGCTAAATTCGTATCCTCGAAAATTAAATCGTTACATAATTTATTAAACTATGACTTTTCGCAATTAATTGATTATCATGAAATAGGAGATAAGATTATAAATTCTATAATTTCTTGA", "aa": "MASDLFKKVKHKRPMLSLNKAYSLDEIQKFIDNIKKITSDFSFFIEPKIDGLSISIIYEDGKLVRAITRGDGITGEDVTANVIQMSCIPKTIKYKKSLEVRGEVYLAISDFEKLNLKLGQENKNKLANPRNAAAGTLRQLNSQIIKERNLSAFLYYVVDPLQHNIKTMQEEHEFLRTYGFPVTPEVQHANTLQDIEEYIRNFKNQKLLIDYETDGVVIKLNEVQYFDKLGSTAKFPHYAIAFKYEPNVETTTIKRIYATVGRTGLVTYNAELEPVEISGSLVSFATLNNFQYIKDLNLNENDLVYIKKAGEIIPCVIGLISGKKEIDHFQKVEFCPYCHNKLVDSETGLEQFCSNDQCPEIQRRKLIHFCSKDALDINTMGERNIDLFLKNSLISNPIDIFNLKNKKQDLLKLNRMGEKSIENILISIDECKNKPLYKMISALSIPLIGVKLAKFVSSKIKSLHNLLNYDFSQLIDYHEIGDKIINSIIS", "aa_hexdigest": "1edf93a97fdf6abed2f4b212badf4fae", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BDB6CC", "query_cov": 0.9938775510204082, "subject_cov": 0.8513986013986014, "identity": 0.602, "score": 605.0, "evalue": 1.39e-211, "valid": false, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_A0AAJ5IJW6", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAJ5IJW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5IJW6" ], "product": "DNA ligase" }, "genes": [], "id": "AHCKFKLKGB_433", "locus": "GCA013367975_00409" }, { "type": "cds", "sequence": "contig_1", "start": 233005, "stop": 233226, "strand": "+", "frame": 1, "gene": null, "product": "BRCT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE292E9" ], "nt": "TTGGAAAATAAATCATTTGTAATAACAGGAACATTGGGACATCCTAGATCATATTTCGAAAGGTTAATTGAACAAAATGGTGGTTTAATTCACTCAAGTATTTCATCTAAAACTAATTATTTATTAGCAGGTGAAAATGCTGGCTCTAAATTGCAAAAGGCAAATAAATTAAATATTACTATAATAACTGAAGAAGATTTATTTAAAATGATTGGTCAATAA", "aa": "MENKSFVITGTLGHPRSYFERLIEQNGGLIHSSISSKTNYLLAGENAGSKLQKANKLNITIITEEDLFKMIGQ", "aa_hexdigest": "5518f639d0aae5b447668bf77664eae0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE292E9", "query_cov": 0.9863013698630136, "subject_cov": 1.0, "identity": 0.653, "score": 94.0, "evalue": 7.12e-24, "valid": false, "product": "BRCT domain-containing protein", "uniref50_id": "UniRef50_UPI002FE292E9" }, "pscc": { "uniref50_id": "UniRef50_UPI002FE292E9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE292E9" ], "product": "BRCT domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_434", "locus": "GCA013367975_00410" }, { "type": "cds", "sequence": "contig_1", "start": 233203, "stop": 233850, "strand": "-", "frame": 1, "gene": null, "product": "MAG0480 family ComEC-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y8", "UniRef:UniRef90_D1J7Y8" ], "nt": "TTGAATTCAAAGGAATTGGTTGTTGATTTGATTTCAATATTAATGTTATTTTTTTACCTAAGGTTATTAAATTTTTCAATATCTGCAAGTCGGGCGTTTTTGTTTTTGATATTAAAAATTGCAAGCAAAAACATTTTTAAAGGCAAATTTAATAACATTGACATATTGAGTTTTACAGCACTATTATTGATATTCTTTGATATTAGAACCATGTATTCTTATTCTTTTATTTTATCTTTTACAATAACATTGATTATTTTTTTAATTTTAGACATTAAAAAAATTTACAAAAACAATATATTTGTAAATTTTCTATTTATCATATTACCTTATATTTCTTCTATTTCAATAGTAATTTCATTTAATCCAAAATTCAACATTTTAGGAATATTTTATCAATTATTCTTTCTACCAATTTCAATATTTAACTATGTATTTTGTTTATTTGCTTTTATAGTTAAACCTATAATAAAAATGTATGTTTCAATATTTGAAATAGCAATAAATACTTTAAGCTATGGTTGTTTGATTATTGAGGTAGAAAAAAATTCAATATTAGGATATTCAATTAATTTTTATATTATTTCAATATATACATATATTAAAATAAAAAGAGAATATTATTTATTGACCAATCATTTTAAATAA", "aa": "MNSKELVVDLISILMLFFYLRLLNFSISASRAFLFLILKIASKNIFKGKFNNIDILSFTALLLIFFDIRTMYSYSFILSFTITLIIFLILDIKKIYKNNIFVNFLFIILPYISSISIVISFNPKFNILGIFYQLFFLPISIFNYVFCLFAFIVKPIIKMYVSIFEIAINTLSYGCLIIEVEKNSILGYSINFYIISIYTYIKIKREYYLLTNHFK", "aa_hexdigest": "27bf338f087f20093d75da38dd9d1a4c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 233203, "stop": 234150, "observations": { "mutation": [ 233853 ], "stop": [ 233853 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Y8", "query_cov": 1.0, "subject_cov": 0.4725274725274725, "identity": 0.977, "score": 374.0, "evalue": 9.21e-127, "gene_start": 1, "gene_end": 215, "reference_start": 241, "reference_end": 455, "reference_sequence": "MTGIGKFTRSRWKNFNYIKKSFNNLNTNLFVALSFCLYCLIIYKINIVISSIMLSLIIAVNFFNLKYWIKIAIVFPLIFLVHYLWYLDLFKINGKNICGKFEIKRFFNKSILIKYQYYNVLVKNWSSWEFNNFYKYNIELSGTLSIIKNQNMFSLSNKIDFELLNVKLVSKAYAYQEKIPILQDYDYKTKKFLALVLFNIQQANDLFVDQLKQLSVMHYFTISGFHFGIIYLLISKILKWLNSKELVVDLISILMLFFYLKLLNFSISASRAFLFLILKIASKNIFKGKFNNIDILSFAALLLIFFDIRTMYSYSFILSFTITLIIFLILDIKKIYKNNIFVNFLFIILPYISSISIVISFNPKFNILGIFYQLFFLPISIFNYVFCLFAFIVKPIIKMYVSIFEIIINTLSYGCLIIEVEKNSILGYSINFYIISIYTYIKIKREYYLLTNHFK" }, "paralog": false, "identity": 0.9809523809523809, "score": 596.0, "evalue": 1.02e-216, "description": "Internal stop codon at 233853. Nonsense mutation around 233853.", "psc": { "uniref90_id": "UniRef90_D1J7Y8", "product": "MAG0480 family ComEC-like protein", "uniref50_id": "UniRef50_D1J7Y8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Y8", "UniRef:UniRef50_D1J7Y8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Y8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y8" ], "product": "ComEC/Rec2-related protein domain-containing protein" } }, "id": "AHCKFKLKGB_435", "locus": "GCA013367975_00411" }, { "type": "cds", "sequence": "contig_1", "start": 233851, "stop": 234117, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAGTTTGAGCAATAAAATTGATTTTGAATTATTAAATGTTAAATTGGTTTCAAAAGCATATGCATATCAAGAAAAAATTCCAATATTGCAAGATTATGATTATAAAACAAAAAGATTTCTAGCACTGGTTTTATTCAACATTCAACAAGCGAATGATTTGTTTGTAGATCAATTAAAACAACTAAGTGTTATGCACTATTTTACAATAAGTGGATTTCATTTTGGAATAATATATTTGCTTATATCAAAAATTTTAAAATGA", "aa": "MFSLSNKIDFELLNVKLVSKAYAYQEKIPILQDYDYKTKRFLALVLFNIQQANDLFVDQLKQLSVMHYFTISGFHFGIIYLLISKILK", "aa_hexdigest": "c867fefe6e6dc67d1e91726f86160225", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10398.183299999997, "isoelectric_point": 9.188630485534667 }, "id": "AHCKFKLKGB_436", "locus": "GCA013367975_00412" }, { "type": "cds", "sequence": "contig_1", "start": 234567, "stop": 235007, "strand": "-", "frame": 2, "gene": null, "product": "MAG0490 family ComEA-like DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y9", "UniRef:UniRef90_D1J7Y9" ], "nt": "ATGAATAAACGAAAAATTTTTTTGGGACTAGGATTATTTTCATCAGCATCAATAATAATACTAATGTCAATAGGAATTAATAAATACATTTCAAATATAAAAAATAACAATAAATTATTTATCAAAAACAAAAATGAAATAATAACAATAGGAATATCAGGCGCAATAGAATATCCCGACTCATACGAAATTAAAAAAGGCACTAAATTATTGGAGATAATAAAAAAAGCACGTCTTAAAGCGGGCGCAGATTTAAACAATATTGATTTAAATTTAGAGTTAGAAAAAAATCAATCAATTAATATTCCTTTTAAAAAAGATAATTCAATTTCTATTAGAGAAGTAAATAACGTTGGCATATTAGAGGATTTAGGAATAAAAAAAGATATAGCAAAAATTATTTTAAGCTACTTCATTGCTAAGGATTATTTAATAACATGA", "aa": "MNKRKIFLGLGLFSSASIIILMSIGINKYISNIKNNNKLFIKNKNEIITIGISGAIEYPDSYEIKKGTKLLEIIKKARLKAGADLNNIDLNLELEKNQSINIPFKKDNSISIREVNNVGILEDLGIKKDIAKIILSYFIAKDYLIT", "aa_hexdigest": "535512cddf6ab2c1e301ed97e075a0ac", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_D1J7Y9", "query_cov": 1.0, "subject_cov": 0.8588235294117647, "identity": 0.973, "score": 264.0, "evalue": 1.27e-88, "valid": true, "product": "MAG0490 family ComEA-like DNA-binding protein", "uniref50_id": "UniRef50_D1J7Y9" }, "pscc": { "uniref50_id": "UniRef50_D1J7Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y9" ], "product": "ComEA family DNA-binding protein" }, "genes": [], "id": "AHCKFKLKGB_437", "locus": "GCA013367975_00413" }, { "type": "cds", "sequence": "contig_1", "start": 235149, "stop": 235790, "strand": "-", "frame": 2, "gene": "tACO1", "product": "YebC/PmpR family DNA-binding transcriptional regulator", "db_xrefs": [ "COG:COG0217", "COG:KJ", "SO:0001217", "UniRef:UniRef50_Q98PU5", "UniRef:UniRef90_D1J7Z0" ], "nt": "ATGTTTCAAAAATTTTCAAAAGAAATTATTGTTGCTGCAACATTAGGCGGACCAGATCCAGACTCTAACCCATCGCTAAAATTAGCAATTGCTAAGGCTAAGGCAAAAAGTATGCCTAAAGCAAACATTGAAAAGGCAATTGCAAAAGTAGCTGGAGGATCTAAAGAAGCTGCAAACTATCAATCTTATTTATATTCAGGTACAGCATTTGGCGGTGTAAATTTTATTGTAAGTTGTTTAACAGATAATTTCAATCGTTTAAGCTCAAATATCAAACATTTCTTTTCAAAACACAATGGTCAATTAGGAAAACAAGGAATAATACCATATGTTTTTGATCAAAAAGGTTTAATTGAATTTCCAAGTAATGTTTCTGGTGAAGAAGAAACCATGATGACAGTTTTAGATGCGGGAGCAGAAGATTTTAGAAATGAAGATGGAACTTATACAATAATTACAAGCCCTTCAGCATTTCAAAAAGTAAAACAAGCATTAGATGAAACATTCAAAATTGAAAATTATACAACAGCTGAAGTTACATATATCCCAAATACCGAAATTGAAGTAGATGAAGAAAAAGCAAGCCAAATTGAACAATTTGTTGACTTTTTAGAAGACGATGAAGACGTCCAAGAAGTTTGA", "aa": "MFQKFSKEIIVAATLGGPDPDSNPSLKLAIAKAKAKSMPKANIEKAIAKVAGGSKEAANYQSYLYSGTAFGGVNFIVSCLTDNFNRLSSNIKHFFSKHNGQLGKQGIIPYVFDQKGLIEFPSNVSGEEETMMTVLDAGAEDFRNEDGTYTIITSPSAFQKVKQALDETFKIENYTTAEVTYIPNTEIEVDEEKASQIEQFVDFLEDDEDVQEV", "aa_hexdigest": "40ff67e088fb0f542b927f88be3c8775", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_D1J7Z0", "query_cov": 1.0, "subject_cov": 0.8765432098765432, "identity": 1.0, "score": 400.0, "evalue": 2.78e-140, "valid": true, "gene": "TACO1", "product": "YebC/PmpR family DNA-binding transcriptional regulator", "uniref50_id": "UniRef50_Q98PU5", "cog_id": "COG0217", "cog_category": "KJ" }, "pscc": { "uniref50_id": "UniRef50_Q98PU5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PU5" ], "product": "Probable transcriptional regulatory protein MYPU_6240" }, "genes": [ "tACO1" ], "id": "AHCKFKLKGB_438", "locus": "GCA013367975_00414" }, { "type": "cds", "sequence": "contig_1", "start": 235944, "stop": 236906, "strand": "+", "frame": 3, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "RefSeq:WP_175933442.1", "SO:0001217", "UniParc:UPI00158B22DD", "UniRef:UniRef100_UPI00158B22DD", "UniRef:UniRef50_A0A454C9E8", "UniRef:UniRef90_A0A454C9E8" ], "nt": "ATGTATTTAATAAGAGGAGAAGATAGTTATTTAATAAATCAAGAATTACAAAAAATTATTGATTTAAAATTTGAAAATCAAGACGATTATTCTGTAATTACTTACAATGAATCTATTGATTTAGAGCAACTATCAGATGATATATTTAACCAATCATTATTTTCTTTTAAAAAACAAGTCATAGTTTTAAAAAACATTGATTTTTTTAATTCTAAGAAAAAAAATGACAATAAAAAAATGAACGATTTTTTACAATTGCTTGAAAAAGGGCAAGATAACAATTTAATATTTATTTTGCAAGATATTAAAAAAAATGATATTTCTTTTAATCCTTCATTGGCTTATAAAAAAATTGAATATATTTCAAAAATAATTGAAATTAAAAAACCAAGTGATAAGGATGTATATAACTATATAAAGGAATATATTTTAAAAAATGGAGGCAATATATCTTCAACTACATTACTTGATTTTTTGTCAATGTTTCCTAATGATTTACAACAAATTATTAATGAAGTAGATAAATTGCTATTGCAAAATAAAAATATTGATATGTTGTTGATCAATAATTCAAATATTATTTCTTCTGACAACATTGAATTTAATTTATCAAATTGTATTTTAAAACAAAAAAGCATTGATATTATAGAAGCAGTAGATGAACAAAAGAAATATGGTAATGGTGCTAGTGAAATTCTAAGCCAAATAAGTTATTTGTTTTATCAAATTTATTGCACGTATTTGTTGATGGAAAATACCAGTGATAATGAATATATATCAAAAACTTTATCAATACATTCATATAGAATCAAGTTGTTTATGTCGTATATAAAAAAAACAAACAAGCATAAATTAAAGGAATTAATAGAGTTACTTGCTGATATAGATATAGCAATTAAATCAGGATTATTAGATGAAGAAAATGCCTTAAATAAATTTATTTTATTTTACATAGAAATATAA", "aa": "MYLIRGEDSYLINQELQKIIDLKFENQDDYSVITYNESIDLEQLSDDIFNQSLFSFKKQVIVLKNIDFFNSKKKNDNKKMNDFLQLLEKGQDNNLIFILQDIKKNDISFNPSLAYKKIEYISKIIEIKKPSDKDVYNYIKEYILKNGGNISSTTLLDFLSMFPNDLQQIINEVDKLLLQNKNIDMLLINNSNIISSDNIEFNLSNCILKQKSIDIIEAVDEQKKYGNGASEILSQISYLFYQIYCTYLLMENTSDNEYISKTLSIHSYRIKLFMSYIKKTNKHKLKELIELLADIDIAIKSGLLDEENALNKFILFYIEI", "aa_hexdigest": "3c3070d35d89bf16e8cf73614ba4f4cc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158B22DD", "ncbi_nrp_id": "WP_175933442.1", "uniref100_id": "UniRef100_UPI00158B22DD", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B22DD", "RefSeq:WP_175933442.1", "UniRef:UniRef100_UPI00158B22DD" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B22DD", "uniref90_id": "UniRef90_A0A454C9E8" }, "psc": { "uniref90_id": "UniRef90_A0A454C9E8", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A454C9E8", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9E8" ], "product": "DNA polymerase III subunit delta" }, "genes": [ "holA" ], "id": "AHCKFKLKGB_439", "locus": "GCA013367975_00415" }, { "type": "cds", "sequence": "contig_1", "start": 236917, "stop": 237102, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTTCTATTTATGAACTTAATTTAAGCACTTTTAATAATAAAAAGGGTTTAAACAAGTATTTGTCAATTATTGAAAAAATTGAATATTTAAAATCATTAAATATTGATATCATTGCAATTGATGATATATTATTGAAATGCTATGAAATCAATAGAAATTATGAATTTAATGATTGA", "aa": "MKKISIYELNLSTFNNKKGLNKYLSIIEKIEYLKSLNIDIIAIDDILLKCYEINRNYEFND", "aa_hexdigest": "992aac5f3fde38e0dba9e482c0ac1027", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7290.408999999998, "isoelectric_point": 6.194790840148925 }, "id": "AHCKFKLKGB_440", "locus": "GCA013367975_00416" }, { "type": "cds", "sequence": "contig_1", "start": 237109, "stop": 237513, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATTTTAAAAAATTAGTTGAACTTTGTTCGGCAAATAACATTAAAATAATGCCAACAATCAATATTGCAAAATTAAAAAAAAGTATTATTGCAAAAAAGCATTTAATTTCTATTTATCGAAACAAAAGCAGTGAAAATGAATCAAATAAAAATGATATTGAATCTTATTTATTAACCCAAGAATTTCTAGTTCCCACCATTGAAAATATTTCAAATTTAAGCGTTTTCTTAAAAGAAATTATTAGTTTTTATAGTGTATTAGGCATCAAGGGATTTATTCTTGAAGATTTTGAATTCTTAATCAATAAAAAAACAAATAACATGAAGTCAAAATTTAGTTTTTTATTAGATATATATAGAATTATCAAACACATAAATTTTAGTTCTATGGTTATTTGA", "aa": "MQDFKKLVELCSANNIKIMPTINIAKLKKSIIAKKHLISIYRNKSSENESNKNDIESYLLTQEFLVPTIENISNLSVFLKEIISFYSVLGIKGFILEDFEFLINKKTNNMKSKFSFLLDIYRIIKHINFSSMVI", "aa_hexdigest": "ed00c073e64af204aa9e4a35fc3b7cf2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15586.232699999988, "isoelectric_point": 9.408274269104005 }, "id": "AHCKFKLKGB_441", "locus": "GCA013367975_00417" }, { "type": "cds", "sequence": "contig_1", "start": 237805, "stop": 238290, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAATGCTTGCAAAAAATTCTTATAGTTTATACAATGGCGATGAATTAGGAATTCTTGGTACTGATTCAAATAATATTTTTAATTTAAATAACATTAATTTTAACGAAGAAAAAAGATTTTATCAATCTAGGAATATTTCTAGTCAAAAATATATTGAGTCTCAAATTAAATTCAATCCAATAACACTACAAATAGAATTTCCATGGACTAATAACATTCAAAATTTAGATTTTATACAGCCAAAATTGCGTAACAAAATTAATGTTGAACAACAGTTAACCAGTAAAGATAGCACGTTTAATTTTTATTCAAATTTAGTTAGAATTTTAAACAAATCAAAATATTCAATTTATTTTAAGGGTTTTCCCAAAGTTTTACTTTGCAATAATTTAATAAGTATTGAATATAGTACTAGTGAAAATAAAAAACTTTTAATTTTATTAAACTTAAATTCGTTTCCAATAAAATCATATATATGA", "aa": "MFLMLAKNSYSLYNGDELGILGTDSNNIFNLNNINFNEEKRFYQSRNISSQKYIESQIKFNPITLQIEFPWTNNIQNLDFIQPKLRNKINVEQQLTSKDSTFNFYSNLVRILNKSKYSIYFKGFPKVLLCNNLISIEYSTSENKKLLILLNLNSFPIKSYI", "aa_hexdigest": "83018acfdff413be1b2ea1d7dc6981ef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18949.495500000008, "isoelectric_point": 9.361405754089354 }, "id": "AHCKFKLKGB_442", "locus": "GCA013367975_00418" }, { "type": "cds", "sequence": "contig_1", "start": 238450, "stop": 238572, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTACAATTCGATGCATCACAAGTAGCAAAGGAACAAAAGGCGTCAACAGTTGGTTCTGCAATTGTTTATACACTTTTAGCTATAGTTTTGGCTGCCGTCTTAGCTTTTTTAATTTGA", "aa": "MQLQFDASQVAKEQKASTVGSAIVYTLLAIVLAAVLAFLI", "aa_hexdigest": "91c0d7081103885315333806e60440c0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4222.982499999998, "isoelectric_point": 5.8228364944458 }, "id": "AHCKFKLKGB_443", "locus": "GCA013367975_00419" }, { "type": "cds", "sequence": "contig_1", "start": 238621, "stop": 238887, "strand": "+", "frame": 1, "gene": null, "product": "DUF4129 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Z3", "UniRef:UniRef90_D1J7Z3" ], "nt": "ATGCAAAAACAAAAAGAAACGTTAGCACTATTTTATCAATATATTTTAAGCTTTTATGAAGTTATAAAATTTACAAACCAAGAACTTAAAAATTTTGAAGTTTCAATAAGCACTCATCCAATGGGTGAAATTAAAGAAGGCGCTAAAAGGTTACTATATCGTTTAATTACAAGAGATGATTTTTCATATTCATTTGTTAAAAATGAACAATACAAAACCTTTGTAAAACACGCCGAATTAATAAATATTACAAATTGCAACTTATGA", "aa": "MQKQKETLALFYQYILSFYEVIKFTNQELKNFEVSISTHPMGEIKEGAKRLLYRLITRDDFSYSFVKNEQYKTFVKHAELINITNCNL", "aa_hexdigest": "9b36e6ba1a2caa28196c4251ad6c657f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 238450, "stop": 239019, "observations": { "mutation": [ 238570, 238885 ], "stop": [ 238570, 238885 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Z3", "query_cov": 1.0, "subject_cov": 0.4656084656084656, "identity": 1.0, "score": 177.0, "evalue": 3.99e-55, "gene_start": 1, "gene_end": 88, "reference_start": 58, "reference_end": 145, "reference_sequence": "MQLQFDASQVAKEQKASTVGSAIVYTLLAIVLAAVLAFLIWKLIKNKVLKKRAQKVEMQKQKETLALFYQYILSFYEVIKFTNQELKNFEVSISTHPMGEIKEGAKRLLYRLITRDDFSYSFVKNEQYKTFVKHAELINITNCNLWDKKIPETINYFKEQYELVPLGKTRDDYIQLVQKSISERFYNEK" }, "paralog": false, "identity": 0.9894179894179894, "score": 360.0, "evalue": 7.01e-130, "description": "Internal stop codon at 238570, 238885. Nonsense mutation around 238570, 238885.", "psc": { "uniref90_id": "UniRef90_D1J7Z3", "product": "DUF4129 domain-containing protein", "uniref50_id": "UniRef50_D1J7Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Z3", "UniRef:UniRef50_D1J7Z3" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Z3" ], "product": "DUF4129 domain-containing protein" } }, "id": "AHCKFKLKGB_444", "locus": "GCA013367975_00420" }, { "type": "cds", "sequence": "contig_1", "start": 239231, "stop": 239467, "strand": "+", "frame": 2, "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "db_xrefs": [ "EC:2.7.8.5", "GO:0008444", "GO:0008654", "GO:0016020", "SO:0001217", "UniRef:UniRef50_UPI001E3AA40F", "UniRef:UniRef90_UPI001E3AA40F" ], "nt": "ATGATAACTGATTTCTTAGATGGATTTTATGCTAGAAAAACAAAGACCGTATCTTCATTTGGAAAGATATTTGATCCAATTGCTGATAAAATTGCAACTACATTGATGTTATTATTCTTAGCGACATATAATTTTGTTTATATACCTATTGCAATTTTGTTTATTATAAGAGACATAATTGTTGATGGTACTAGAGTATATGCTGTAAAGAAAAACATTGAAGTATCAGCAAATTGA", "aa": "MITDFLDGFYARKTKTVSSFGKIFDPIADKIATTLMLLFLATYNFVYIPIAILFIIRDIIVDGTRVYAVKKNIEVSAN", "aa_hexdigest": "99081ee91c5acbeb6bb892c98e69355e", "start_type": "ATG", "rbs_motif": null, "genes": [ "pgsA" ], "truncated": "both", "pseudogene": { "start": 239009, "stop": 239543, "observations": { "insertion": [ 239525 ], "mutation": [ 239069, 239201, 239465 ], "stop": [ 239069, 239201, 239465 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E3AA40F", "query_cov": 1.0, "subject_cov": 0.4406779661016949, "identity": 0.9490000000000001, "score": 143.0, "evalue": 6.13e-42, "gene_start": 1, "gene_end": 78, "reference_start": 75, "reference_end": 152, "reference_sequence": "MKNKSQKKQKIIVSKFGVANWLTVLRMLLMIPFIVFLSTSFGLILNGVQLSYKTNKGIVLSILYWINVAIFIIAMITDFLDGFYARKTKTVSSFGKIFDPIADKVATTLMLLFLATYNFVYIPIVILFIIRDIIVDGTRVYAIKKNIEVSTNWFGKIKTILVSLALVVISIGFLLYR" }, "paralog": false, "identity": 0.9548022598870056, "score": 312.0, "evalue": 3.32e-111, "description": "Internal stop codon at 239069, 239201, 239465. Frameshift due to insertion around 239525. Nonsense mutation around 239069, 239201, 239465.", "psc": { "uniref90_id": "UniRef90_UPI001E3AA40F", "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "ec_ids": [ "2.7.8.5" ], "uniref50_id": "UniRef50_UPI001E3AA40F", "go_ids": [ "GO:0008444", "GO:0008654", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E3AA40F", "EC:2.7.8.5", "UniRef:UniRef50_UPI001E3AA40F", "GO:0008444", "GO:0008654", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E3AA40F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3AA40F" ], "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase" } }, "id": "AHCKFKLKGB_445", "locus": "GCA013367975_00421" }, { "type": "cds", "sequence": "contig_1", "start": 239663, "stop": 239776, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTCAATCATATACAAAAAATGATTCAGATGCAACAATTGAAAATAATCAAGATCAAAATAATAATAATAATAAATCAAATCAAGATTCTAGCCCATCGCCTAATTGCTAA", "aa": "MLQSYTKNDSDATIENNQDQNNNNNKSNQDSSPSPNC", "aa_hexdigest": "6002f9348f219b2eb93c12a22c80da26", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4131.173500000002, "isoelectric_point": 4.0500284194946286 }, "id": "AHCKFKLKGB_446", "locus": "GCA013367975_00422" }, { "type": "cds", "sequence": "contig_1", "start": 239932, "stop": 240276, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B181AC3", "UniRef:UniRef90_UPI000B181AC3" ], "nt": "ATGAAATCAAAGAAAATAAAGTTATTAAAGTTATTATTAACATCGTCAGTTGCAATTTCGTGTGCAATATTACCTGTTTCTTGTTCTTTATTATCTAATGATAATGATAAAAAAATAACAATAAAGAATTATGAATTTAATAATTTATCAAGTGAATACACAATTGAATCTAAAAAATTAAATTTATCATTTGTTAATGATGGCAATGTTCCATATGTTAATTTGCTACAAATGATAAATTCATTAGATGGATTTTTTGATAAAAACTATATTAATTTTTCAACAAATATTCTATTTTCTGAATTGAAAATGGCAACTAAATTTGGATATATTTCGTTTAACTGA", "aa": "MKSKKIKLLKLLLTSSVAISCAILPVSCSLLSNDNDKKITIKNYEFNNLSSEYTIESKKLNLSFVNDGNVPYVNLLQMINSLDGFFDKNYINFSTNILFSELKMATKFGYISFN", "aa_hexdigest": "8d544c72682c98da92f65c86ef2c4fa2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI000B181AC3", "query_cov": 1.0, "subject_cov": 0.8837209302325582, "identity": 0.982, "score": 214.0, "evalue": 4.04e-70, "valid": true, "uniref50_id": "UniRef50_UPI000B181AC3" }, "pscc": { "uniref50_id": "UniRef50_UPI000B181AC3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B181AC3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12922.893599999983, "isoelectric_point": 9.077035713195801 }, "id": "AHCKFKLKGB_447", "locus": "GCA013367975_00423" }, { "type": "cds", "sequence": "contig_1", "start": 240295, "stop": 240822, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D962DE", "UniRef:UniRef90_UPI0034D962DE" ], "nt": "ATGCCAAACACTTATTATGAATATATAACAAAAAGCCCAGGAAAGACTAATTTCAACAAATATTTGAAATCCAAATCATATTATTCAAAGGATTTTAATAAAAATACAATTGTTATAGATTTAAAAAAATATAATTTTGACATTTTATATCATAACGGTAATGTTTTGCTGCCATTAGCAATATTTAATTTGTTATTTTGCTCAACTAATTATTACAATTTGTATTTCAATGGTAACAAAATCTATGGGTGCGATTTTGACATTAAATCTAATGCTAAAGAAATTCCTCAAATTAGAAATAGCAAATTTCAAAACTCACTTATTCCCAAGGATATAGTTGAAGCAAAATTTAACTTCACTGCCTTACTAATTGATTATTTTTACGGCCTTAAAGAATATAATAATATTAATAGTTTTTATTTCACAATTTCAAATGAACAAAAGCAAAGAATGCTTAGTCAAAATCCAGTTATTTCAACAAAGGAATATATAAAATATTTATACAATTTAAATGATTTACACACCTGA", "aa": "MPNTYYEYITKSPGKTNFNKYLKSKSYYSKDFNKNTIVIDLKKYNFDILYHNGNVLLPLAIFNLLFCSTNYYNLYFNGNKIYGCDFDIKSNAKEIPQIRNSKFQNSLIPKDIVEAKFNFTALLIDYFYGLKEYNNINSFYFTISNEQKQRMLSQNPVISTKEYIKYLYNLNDLHT", "aa_hexdigest": "676993b76c5022ee6466439531a88be9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 239995, "stop": 240882, "observations": { "mutation": [ 240274, 240820 ], "stop": [ 240274, 240820 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D962DE", "query_cov": 1.0, "subject_cov": 0.5537974683544303, "identity": 0.966, "score": 342.0, "evalue": 5.25e-117, "gene_start": 1, "gene_end": 175, "reference_start": 122, "reference_end": 296, "reference_sequence": "MKSKKINLLKLLLTSSVAISCAILPVSCSLLSNDNDKKITIKNYEFNNLSSEYTIESKKLNLSFANDGNVPYVNLLQMINSLDGFFDKNYINFSTNILFSELKITTKFGYISFNWKTNKITMPNTYYEYITKSPGKTNFNKYLKSKSYYSKDFNKNTIVIDLKKYNFDILYHNGNVLLPLAIFNLLFCSTNYYNLYFNGNKIYGCDFDIKSNVQEIPQIRNGKFQNSLIPNDIVEAKFNFTALLIDYFYGLKENNNINSFYSTISNEQKQRMLSQNPVISTKEYIKYLYNLNDLHTWVSMPSFYNRKDSSIAKDFY" }, "paralog": false, "identity": 0.9593220338983051, "score": 575.0, "evalue": 2.21e-211, "description": "Internal stop codon at 240274, 240820. Nonsense mutation around 240274, 240820.", "psc": { "uniref90_id": "UniRef90_UPI0034D962DE", "uniref50_id": "UniRef50_UPI0034D962DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D962DE", "UniRef:UniRef50_UPI0034D962DE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D962DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D962DE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20834.505400000005, "isoelectric_point": 9.162262916564941 }, "id": "AHCKFKLKGB_448", "locus": "GCA013367975_00424" }, { "type": "cds", "sequence": "contig_1", "start": 240829, "stop": 240957, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E356EC4", "UniRef:UniRef90_UPI001E356EC4" ], "nt": "ATGCCTTCATTTTATAATCACAAGGATTCTAGCATTGCAAAAGATTTCTATGAATATACCACAAATAAAAGAGAAAGAATTTATCAATCTACAATAAAATATCTAAAAAATAATAAATTAAATAATTGA", "aa": "MPSFYNHKDSSIAKDFYEYTTNKRERIYQSTIKYLKNNKLNN", "aa_hexdigest": "ff78031624260bd747f455f2684c0b03", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 240745, "stop": 240939, "observations": { "mutation": [ 240820 ], "stop": [ 240820 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E356EC4", "query_cov": 0.8571428571428571, "subject_cov": 0.5373134328358209, "identity": 0.9440000000000001, "score": 71.2, "evalue": 1.69e-15, "gene_start": 1, "gene_end": 36, "reference_start": 29, "reference_end": 64, "reference_sequence": "MLSQNPVISTKEYIKYLYNLNDLHTWVSMPSFYNRKDSSIAKDFYEYTTNKRERIYQSTRKYLKIIN" }, "paralog": false, "identity": 0.953125, "score": 124.0, "evalue": 2.74e-39, "description": "Internal stop codon at 240820. Nonsense mutation around 240820.", "psc": { "uniref90_id": "UniRef90_UPI001E356EC4", "uniref50_id": "UniRef50_UPI001E356EC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E356EC4", "UniRef:UniRef50_UPI001E356EC4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E356EC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E356EC4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5164.719000000001, "isoelectric_point": 9.573119926452637 }, "id": "AHCKFKLKGB_449", "locus": "GCA013367975_00425" }, { "type": "cds", "sequence": "contig_1", "start": 241132, "stop": 241371, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAATCAAGAAAAACCTAAGAAATATGAAAATAAAAAACATTATTGTAGATTTATCAACCAATGGTGGTGGCGATATGGGTGCTATGCAAAGGGGGTTGGGTTTTATCACAGATGAACCAATATTAAACAAAACATATAATCGTTTAAATGAAGAAATAAATATTAGATCTGTTGAAGTTGATATAGATGGCGATGGAAATTACAAAAATGATGCTTATTCTGAATACAATTGA", "aa": "MKLIKKNLRNMKIKNIIVDLSTNGGGDMGAMQRGLGFITDEPILNKTYNRLNEEINIRSVEVDIDGDGNYKNDAYSEYN", "aa_hexdigest": "176a4fc7fb9b14db2b815b65a83b54fb", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8954.036399999994, "isoelectric_point": 5.294972801208496 }, "id": "AHCKFKLKGB_450", "locus": "GCA013367975_00426" }, { "type": "cds", "sequence": "contig_1", "start": 241435, "stop": 241665, "strand": "+", "frame": 1, "gene": null, "product": "S41 family peptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005133DA3", "UniRef:UniRef90_UPI0005133DA3" ], "nt": "ATGAAATTAGGCAAGATAATAGGACAAAAAACAGGCGGCGGTGCAGCATCAATTTTACCTGTTGTGTTGCCAGATGGAACTTCAATAGTAATAAGTTCTAATAATTTATCTGTAACTATGAAAAATAATACAATTAAATCTATTGAAGGTGGAATAGAACCTGATATTTACTTAGAATACAAAGATTTTTATAATGATCAAAAACTATTAGAAATAATAAAAAATAAATAA", "aa": "MKLGKIIGQKTGGGAASILPVVLPDGTSIVISSNNLSVTMKNNTIKSIEGGIEPDIYLEYKDFYNDQKLLEIIKNK", "aa_hexdigest": "acdb24b4ee0f58b34eb6fcedffcaac12", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 241333, "stop": 241665, "observations": { "mutation": [ 241369 ], "stop": [ 241369 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005133DA3", "query_cov": 1.0, "subject_cov": 0.628099173553719, "identity": 0.987, "score": 147.0, "evalue": 1.53e-44, "gene_start": 1, "gene_end": 76, "reference_start": 46, "reference_end": 121, "reference_sequence": "MGLTPHFSTLPGDYKNDAYSEYNWYLLTGVNTFSAANTFTSIFKEMKLGKIIGQKTGGGAASILPVVLPDGTSIVISSNNLSVTMKNNTIKSIEGGIKPDIYLEYKDFYNDQKLLEIIKNK" }, "paralog": false, "identity": 0.9636363636363636, "score": 211.0, "evalue": 2.92e-72, "description": "Internal stop codon at 241369. Nonsense mutation around 241369.", "psc": { "uniref90_id": "UniRef90_UPI0005133DA3", "product": "S41 family peptidase", "uniref50_id": "UniRef50_UPI0005133DA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005133DA3", "UniRef:UniRef50_UPI0005133DA3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005133DA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005133DA3" ], "product": "S41 family peptidase" } }, "id": "AHCKFKLKGB_451", "locus": "GCA013367975_00427" }, { "type": "cds", "sequence": "contig_1", "start": 242133, "stop": 242318, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATTCAAATAAAATAACAAAACAAGAATATGAAATTATTTTTAATGAATTTAAAGGAATAATATTGATATTTAAGCAACTAATCACAAATATTTATAGTAAAGATTTAAAAAATGTTTTAACATCTCAACTGCCAAAAGTTTTTAAAGCAATAAATAATGCTAAAAAAAGCGCTAAATAA", "aa": "MVNSNKITKQEYEIIFNEFKGIILIFKQLITNIYSKDLKNVLTSQLPKVFKAINNAKKSAK", "aa_hexdigest": "f5520905232713a6deb00746ece17446", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7060.3039000000035, "isoelectric_point": 9.933240318298338 }, "id": "AHCKFKLKGB_452", "locus": "GCA013367975_00428" }, { "type": "cds", "sequence": "contig_1", "start": 242403, "stop": 242672, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E316352", "UniRef:UniRef90_UPI001E316352" ], "nt": "ATGTTCAAAGAATTTAAAAATGGAACTGATGTTTTTATTAAATATTTAACTCCAATATTGAATAGCTTTAAAGAAAAACATGAATTTTTAAAAACAAATGATAACAAATATAATGAATTAAAAAGCTTGTTAATAAAACATTTAGAATATATTTTGGATTACTTTTGCAATCTTAAATTTGATTATTTATTCCACAATGAAGGTCCAAAACAAACAGAATATGATAATAAAGGTGGAGGAAAGAAAGTATTGATATTATTTGGTGGATGA", "aa": "MFKEFKNGTDVFIKYLTPILNSFKEKHEFLKTNDNKYNELKSLLIKHLEYILDYFCNLKFDYLFHNEGPKQTEYDNKGGGKKVLILFGG", "aa_hexdigest": "87948b8600648fc4c0d5cffcc3b2baf1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 242336, "stop": 242798, "observations": { "insertion": [ 242357 ], "mutation": [ 242405, 242684, 242711 ], "stop": [ 242405, 242684, 242711 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E316352", "query_cov": 1.0, "subject_cov": 0.6013513513513513, "identity": 0.888, "score": 173.0, "evalue": 5.41e-54, "gene_start": 1, "gene_end": 89, "reference_start": 38, "reference_end": 126, "reference_sequence": "MLNVLLELGIDDKVSKGLRNLTRDWVLSKTVGAWAIKMFKEFKNGTDVFIEYLTPILNSFKEKHEFLKTNDSKYNELKSMLIEHLKYILDYFCNLKFDYLFHNEGPKQSQYDKKGGGKMVLLLFGGWPILFDVEKFIEPKYSIHPAMI" }, "paralog": false, "identity": 0.8431372549019608, "score": 253.0, "evalue": 4.64e-88, "description": "Internal stop codon at 242405, 242684, 242711. Frameshift due to insertion around 242357. Nonsense mutation around 242405, 242684, 242711.", "psc": { "uniref90_id": "UniRef90_UPI001E316352", "uniref50_id": "UniRef50_UPI001E316352", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E316352", "UniRef:UniRef50_UPI001E316352" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E316352", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E316352" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10576.07359999999, "isoelectric_point": 8.59184665679932 }, "id": "AHCKFKLKGB_453", "locus": "GCA013367975_00429" }, { "type": "cds", "sequence": "contig_1", "start": 242709, "stop": 243776, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAACGAAATTTAAAAAATTTTGCAAATAACTTTGATTTTGATTTTAAAGATGACACCAAAAAAGAATTAGCAAAATCAATGACAATAGATGAATCAATAGAAAAAGGCTACAATTTCTTTTTTAGAGAAAAAATTAATTTTGGTAGCGGAGAAGAATCTTATATTATTGATGATCTTAAAATTCAAAAAGATTATAAACTACTGGCAAGTGAAAATGCAAATGATATAAATATACAAATAACATTCAAATTAGACGATTATAGTGAAATAAAAACCTTTACTAAATCAGGTTTCAATGACAAAGCAAGCATTGAAAAAATAAAAGAAGAATTAAAAAATGAACCAAAAAGGCTTGACGATTTATTGGATAAACTTACAATGGAATTCACTAAAAATAATGATTTTGAAAAGCGATCTATTGATGTTAAAGATATTACAATTCAAGATTATAGATTTAATCTAAAAGATAGTGATTCTGCAGCTATCTATGAAATAGAGGCGGTTAAATATGATAAAACATTGGGAAAATTAACTTATAAAATTAAGTTAAAGTCAAATAAAAGTTTATTGAATGAATATAGTCAAAATCCAATAGAATCTACTAAATCAAGAACATTTGAATTTTATGGATTTGCTAAGGATGTAAGCGAAATAACTAAAAATTTAGAATCGGATGTAAAAAAATCAAATGAATTTAAAAAATATCTAGAAAATGTTAAATTAAATATTGAAACAAATATTGAAAAAAAGATTAATGAAGCAAGAAAAAAACAACACAAAAAAGCCGCTCATGCAAACGTAATTAAAGCAATAATTGAACAATATAAAAAGTTTAACGAAGAAATAGAAAAAACGTTATTTGAACTAGCAAATAAAGCAATTAATGTATTTGGTCAATTTAATTCTAACAAAGAATCTATTAAAAAACTTTACAACATCTTAATTAATTTAATTATTAACACCAATGATGAAATGTTAAATGTATTATTAGAATTAGGCATTGATGATAAAGTTTCTAATGGTAGGGGTTTTATGTATTATAAAAATCTGACAGCAGACTGA", "aa": "MNKRNLKNFANNFDFDFKDDTKKELAKSMTIDESIEKGYNFFFREKINFGSGEESYIIDDLKIQKDYKLLASENANDINIQITFKLDDYSEIKTFTKSGFNDKASIEKIKEELKNEPKRLDDLLDKLTMEFTKNNDFEKRSIDVKDITIQDYRFNLKDSDSAAIYEIEAVKYDKTLGKLTYKIKLKSNKSLLNEYSQNPIESTKSRTFEFYGFAKDVSEITKNLESDVKKSNEFKKYLENVKLNIETNIEKKINEARKKQHKKAAHANVIKAIIEQYKKFNEEIEKTLFELANKAINVFGQFNSNKESIKKLYNILINLIINTNDEMLNVLLELGIDDKVSNGRGFMYYKNLTAD", "aa_hexdigest": "df96ef028859ca9dfd5e3f6dcbebe4d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 41552.655199999965, "isoelectric_point": 7.602124214172364 }, "id": "AHCKFKLKGB_454", "locus": "GCA013367975_00430" }, { "type": "cds", "sequence": "contig_1", "start": 243876, "stop": 244364, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGCATTAGTTGAACGAGAAAATATTATATCTTTAAAAAATAATGAATATTCAACATCTAAAAAATCTTCAATGATTTCAAAAAATGACTTTGAATTAAACAAGCAAAAAAGTAATGAACTAGCAGGCTTGGAATTAAGCATTTCAGAAATCAACGCCAATGCCAATGGAAAATTAGAAATAACTTTTTCATTATTGAATAAAACATTTAAACATGGAACAATAGAATCAAACAAAAAGACAGTTACATTCAATTTCTTAAGTGATGAAGATTACACATTTAATTTAATTAATAACTTAAGCATTTCAAAAGAATCAACTTATGTAACTGATGGCGTTTATACTAAAGCGGATTTTAAAAATAAAATTGCTAATAATAAACAAGAAATTGCTAAGTTGTTAGGTATTTCTGATTTTCTAAATCAAAACTCAATTATCGAATTGAATCAAGAAAATACAAACGCTATTGTGTCATTAAAAGAATGA", "aa": "MDALVERENIISLKNNEYSTSKKSSMISKNDFELNKQKSNELAGLELSISEINANANGKLEITFSLLNKTFKHGTIESNKKTVTFNFLSDEDYTFNLINNLSISKESTYVTDGVYTKADFKNKIANNKQEIAKLLGISDFLNQNSIIELNQENTNAIVSLKE", "aa_hexdigest": "9b6554d071ec5c16f5d698f4c8fd7fe8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18240.227499999994, "isoelectric_point": 5.3499361038208 }, "id": "AHCKFKLKGB_455", "locus": "GCA013367975_00431" }, { "type": "cds", "sequence": "contig_1", "start": 244485, "stop": 245642, "strand": "-", "frame": 2, "gene": null, "product": "lipoprotein 17-related variable surface protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E4E6412", "UniRef:UniRef90_UPI00193A5D11" ], "nt": "GTGGGATTTAATACATTACTCAACATAGAAAGACAAGAAACATCAAAGAAACTTTCTAATTTAGTTAATTCAATTGATCTAAACGAATTTTTGGCACTTGAAAAGCATAACAAAAAACCTTCAGAAATCAAAAATGTTGATTTCAATAGAAAAAATATTGATGGAGCAATGTTAGTTATTGAAAAAATGCAAGCCAATGACGAAGAAGGAACATTGTTAATTAGTTTTTATTTAAAAAGTTCTAATCCAAAATTCAGCGATTTAAAAACAGAAATCAAAACAGTAAAATTGACAGATTTTTTAACAAATGCACAATTTATTGATAACCAAAGAAAAGAAACAATTGCTTATTTAAATGACCAAATAGGAAAATTAAATATAAAAGTAAGCGATAAGAAACCTGAAACTACTATTAAAAGATTTGCATCTTCAATCAAGCAATCAGATTTAAATATTGAAATTTCAAAGCCAAATAATAATTTTAAGAATATAGAAGTTGTTGTAAAGCAATTAATTTCAAACGATGAAAGTGGAAAATTAAGTATAAGATTAGCAATCAAGCAGTTAAACAAACAAATTAATGAATATTGTGAATCAAGCAAACTTATTGAAGTTGAATTAAGTGGTTACAAAACTAAAACAATGGATAAAAACGAAAAAATAGAATCTTTAAATAAAGTTGCCAAAGAAACAACAACGTTAAGATTAATTTCTTCAAAAAATATTGCCAAAACATTTGCTTCTGATGTTCAAATAAACGATTTTGAAACAAAAATTAGTAATGATTTTTATATTGAAATTTTAGAAATAAGTGAATCAAATGATGATAAAGGAAACTTAAAAATTAAATTTGTTGTTAAAACAAACGAAAAGCAATATAATGATTTAAAATCAAATGAGCAAACTATAATATTGACAGGTTTTTATAAAAAAGAACAATACTTAAAAGCTCGTAAGCAAGAGTTGAATAAATTAATTAGCGATGATAAGATAGAAGCAAATGTAGTTTTTGATAAAGAAAATGATAAGTCAAATATATTGCCTTCAAATGTTGAAGGTAAAGAAATTCATCTAAATATAAGCGATTCAAAATATATTATTGAAGGAAATCCATCAATTATCGCAGATGATGATCAAGGAATTTTGTCAATAAGTTGA", "aa": "MGFNTLLNIERQETSKKLSNLVNSIDLNEFLALEKHNKKPSEIKNVDFNRKNIDGAMLVIEKMQANDEEGTLLISFYLKSSNPKFSDLKTEIKTVKLTDFLTNAQFIDNQRKETIAYLNDQIGKLNIKVSDKKPETTIKRFASSIKQSDLNIEISKPNNNFKNIEVVVKQLISNDESGKLSIRLAIKQLNKQINEYCESSKLIEVELSGYKTKTMDKNEKIESLNKVAKETTTLRLISSKNIAKTFASDVQINDFETKISNDFYIEILEISESNDDKGNLKIKFVVKTNEKQYNDLKSNEQTIILTGFYKKEQYLKARKQELNKLISDDKIEANVVFDKENDKSNILPSNVEGKEIHLNISDSKYIIEGNPSIIADDDQGILSIS", "aa_hexdigest": "11bdcb61951c0be0cfeddc5eb4edb7b5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 244182, "stop": 245642, "observations": { "mutation": [ 244487 ], "stop": [ 244487 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193A5D11", "query_cov": 1.0, "subject_cov": 0.5476529160739687, "identity": 0.948, "score": 675.0, "evalue": 7.26e-239, "gene_start": 1, "gene_end": 385, "reference_start": 1, "reference_end": 385, "reference_sequence": "MGFNTLLNIERQETSKKLSNLVNSIDLNEFLVPEKRNKKPSEIKNVDFNRKSIDGAMLVIEKMQANDEEGTLLISFYLKSTNPKFSDLKTEIKTVKLTDFLTNAQFINNQRNETIAYLNDQIGNLNIKVSDKKPETTIKRFSSSIKQSDLNIEIPKPNNNLKNIEVVVKQLIPNDESGKLSIRLAIKQLNKQINEYCESSKLIEVELSGYKTKTMDKNEKIESLNKVAKETTTLRLISSKNIAKTFASDVQINDFETKISNDFYIEILEISESNDDIGSLKIKFVVKTKEEQYKDLKSNEQTITLTGFYKKEQYLKACKQELNKLISDDKIEANVVFDKENDKSNILPSNVEGKEIHLNISDSKYIIEGNPSIIADDDQGILSVSWTIKVKEEKFKTFIVSESRISKKITNFLTKKQNEINIEQKRLDALVEQKNIISLKNNEYLTSKKSSMISENDFELNKQKSNELVGLELSISKISPNASGKLEITFSLLNKTFKHGAKESSKKTVTFDFLSDENYTFNLINKLNISKESTYVTDGVYTEADFNNKIANNKQEIAKLLGISDFLNQNSIIELNQESTNAIVSLKKGANTTVKYEFILKIKSANDKLFKKFSGEISIANIMNKRNLINFANKFDFDFKNDNKKELAKLMTIDESIKNATISFLKKKLILVVEKNLILLMIIKFKKIINYWQVEKKILSIYK" }, "paralog": false, "identity": 0.9362139917695473, "score": 868.0, "evalue": 1.9e-317, "description": "Internal stop codon at 244487. Nonsense mutation around 244487.", "psc": { "uniref90_id": "UniRef90_UPI00193A5D11", "product": "lipoprotein 17-related variable surface protein", "uniref50_id": "UniRef50_UPI001E4E6412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193A5D11", "UniRef:UniRef50_UPI001E4E6412" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E4E6412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E4E6412" ], "product": "lipoprotein 17-related variable surface protein" } }, "id": "AHCKFKLKGB_456", "locus": "GCA013367975_00432" }, { "type": "cds", "sequence": "contig_1", "start": 246081, "stop": 246860, "strand": "-", "frame": 2, "gene": null, "product": "lipoprotein 17-related variable surface protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A2EAC", "UniRef:UniRef90_UPI00193A2EAC" ], "nt": "ATGAAAAAGAATACAAAAATCAAAAGCATTTCCATTTTAAGTGCTACTGCCATTTTACTTGTATCAGGAGTCATTTCATCAGCCTGCAACAAGACGCAAGATAAAGAAACTGAAACAACAAAATTAAAAGAACAAACAAGAAATATTAAACCAAATTTAAAACTTTCTATTAAACAAGATAACATACTTCCAAGTGAAATTAATTCATCAAATTTTTATGTTAATAATCTTGATAAAGAAATATCGTTTGAAATCTCTAATATTATTGCAGATGATGTAAACGGGAATGTTATTATAAATTATTATTTTACAAAATCAATAATCGATTCTAAAACAAGACAACCACTGTTAATAAAATCGAATACGTTTTCTCAAAAATATACAGGATTTAAAGCATTGGTTGCTCCTTTAAATTCGTCAATTGAACAAGTTAATCTAGAAATAAATGAATTTAATAAAAAAGACTTTTTGCCTTCGCAACATATTAAATTGCAAAGCAATTTTTCATTTAAAAACATCGATTCCCAATATGAATTAAAAACTGAATATATTGCAAATGATGAACAAGGCAAAATAGAAGTTAAATATTATTATGTACAAAAAGATAAAGATATAAAATCAAAAATTATTTCAAAAACGTTTTATGGTTTTAATTCATTGAATAATATAGCTAATATGCAAAAAGAAGCTAACACTTTAAATAAGTTAGGAAATGAAATATCTTTAAAATTAAGGAAACAAATTAATAAATCAGAAACATATGCTTCAACATTGAATTGA", "aa": "MKKNTKIKSISILSATAILLVSGVISSACNKTQDKETETTKLKEQTRNIKPNLKLSIKQDNILPSEINSSNFYVNNLDKEISFEISNIIADDVNGNVIINYYFTKSIIDSKTRQPLLIKSNTFSQKYTGFKALVAPLNSSIEQVNLEINEFNKKDFLPSQHIKLQSNFSFKNIDSQYELKTEYIANDEQGKIEVKYYYVQKDKDIKSKIISKTFYGFNSLNNIANMQKEANTLNKLGNEISLKLRKQINKSETYASTLN", "aa_hexdigest": "6ab940ad401084e81cba8d6133c06973", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 245778, "stop": 246860, "observations": { "mutation": [ 245936, 246083 ], "stop": [ 245936, 246083 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193A2EAC", "query_cov": 1.0, "subject_cov": 0.6675257731958762, "identity": 0.981, "score": 467.0, "evalue": 9.78e-164, "gene_start": 1, "gene_end": 259, "reference_start": 1, "reference_end": 259, "reference_sequence": "MKKNTKIKSISILSATAILLVSGVISSACNKTQDKETETTKLKEQTRNIKPNLKLSIKQDKILPSEINLSNFYVNNLDKEISFEISNIIADDVNGNVIINYYFTKSIIDSKTRQPLLIKSNTFSQKYTGFKALVAPLNSSIEQVNLEINEFNKKDFLPSQYIKLQSNFSFKNIDSQYELKTEYIANDGQGKIEVKYYYVQKDKDIKSKIISKTFYGFNSLNNIANMQKEANTLNKLGNEISLKLKKQINKSETYASTLNWSDFETSKKLNSAYLRSNGELIFDDINGEVKLNYYFEKQYIDKKTGASYWIKSKVYTTKITGFKSFLKNLNIAVDKVNLSINEKNINTILPSQYIQNLSNFKFDNLNNEYELQKKFSYDDQKGELTISY" }, "paralog": false, "identity": 0.9777777777777777, "score": 680.0, "evalue": 3.25e-250, "description": "Internal stop codon at 245936, 246083. Nonsense mutation around 245936, 246083.", "psc": { "uniref90_id": "UniRef90_UPI00193A2EAC", "product": "lipoprotein 17-related variable surface protein", "uniref50_id": "UniRef50_UPI00193A2EAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193A2EAC", "UniRef:UniRef50_UPI00193A2EAC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193A2EAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A2EAC" ], "product": "lipoprotein 17-related variable surface protein" } }, "id": "AHCKFKLKGB_457", "locus": "GCA013367975_00433" }, { "type": "cds", "sequence": "contig_1", "start": 246943, "stop": 247986, "strand": "-", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175933447.1", "SO:0001217", "UniParc:UPI00158D4ABA", "UniRef:UniRef100_UPI00158D4ABA", "UniRef:UniRef50_D1J7Z7", "UniRef:UniRef90_D1J7Z7" ], "nt": "ATGAAAAAATCCAAAAAAATTTTATTAACTATGGGGGTAGCACTTCCATTATTAGCCTCAACTCCATTGGTTGCTGCAGGTTGCGTAAAAACAGAACCTGAACCTAAACCAGAAGATCAAAAGAACCCAGAAGGTGGAAATCCAGGAACAACACCAGAAAAGAATCCTGAAGGTGAAAAACCAGGAACAACACCCGAAAAGAAGCCTGAAGGTGAAAAACCAGGAACAACACCAGAAGTTAAACCAGAAGAAAAAAGCGAACTTCAAAAAGCAACTGAAGGTATTGCAGATGCTACAGTTAAAGCAAATTTTGAAAGTGAATTAAAGAAATATCGTGAAGAATTAAAAACTAAATCAGAAGCCATTTTAAAGCCAATTATTGAAAATAAAGAAATTAAAGCTGCAGTTTCTGAAAAAGAATTGCCAAATATTACAAAAACATATAATGAAGCAATTTCTAAATTAAATGAAATTTTAGATGATATTATTTTGTCAACATTAAAAGAATACAACACAAATAAAACAAAAGTTTCTGATGGATTAATGACAATTAAAAATTATTTAACAAAAGAAAAAATTTCTGATTTAATTAAAAAAGAAAACGGATTGGCAGATTTCTTGGTTGCATTTGGAAAAAATATTTCTAAATATGCAAAAGCAATTTTTGAAACACTAAAAACAAATGAATCAACAAAATCAATAGCTCAATTAAACGATTTAATTAAAGCTGTTGACGATAAATTTGCTGGAAAAGATAATTTATTTGAAACAACCGCTAAGAAATTAAAAGAATTTGATTTTAGCAAATTGGCTGAAGAAGGATATGTAAAAACATTTGAAAAATTAATTACATTATTATCTGACGCTAAGGCAGAAGCAGTTGCGGTATTAAAATCTGTAAAAATGTCTGATGAAGATATTAAAAAAGTAAAAGACGTAATTAGACCAATTTACAACGTTTTAAATGAAGGTCTAAAAAACTCAATTAACTTATTTGAACAATCATTGAGAGATTTACCAAAGGTGTTAGAAATCTTAAAATAA", "aa": "MKKSKKILLTMGVALPLLASTPLVAAGCVKTEPEPKPEDQKNPEGGNPGTTPEKNPEGEKPGTTPEKKPEGEKPGTTPEVKPEEKSELQKATEGIADATVKANFESELKKYREELKTKSEAILKPIIENKEIKAAVSEKELPNITKTYNEAISKLNEILDDIILSTLKEYNTNKTKVSDGLMTIKNYLTKEKISDLIKKENGLADFLVAFGKNISKYAKAIFETLKTNESTKSIAQLNDLIKAVDDKFAGKDNLFETTAKKLKEFDFSKLAEEGYVKTFEKLITLLSDAKAEAVAVLKSVKMSDEDIKKVKDVIRPIYNVLNEGLKNSINLFEQSLRDLPKVLEILK", "aa_hexdigest": "47d6a40bdfe35833d3b172ff68f8a989", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158D4ABA", "ncbi_nrp_id": "WP_175933447.1", "uniref100_id": "UniRef100_UPI00158D4ABA", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D4ABA", "RefSeq:WP_175933447.1", "UniRef:UniRef100_UPI00158D4ABA" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D4ABA", "uniref90_id": "UniRef90_D1J7Z7" }, "psc": { "uniref90_id": "UniRef90_D1J7Z7", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J7Z7" }, "pscc": { "uniref50_id": "UniRef50_D1J7Z7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Z7" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "AHCKFKLKGB_458", "locus": "GCA013367975_00434" }, { "type": "cds", "sequence": "contig_1", "start": 248346, "stop": 248498, "strand": "+", "frame": 3, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "COG:COG0267", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_012855488.1", "SO:0001217", "UniParc:UPI0001BC21A4", "UniRef:UniRef100_D1J7Z8", "UniRef:UniRef50_P35871", "UniRef:UniRef90_B3PMG6" ], "nt": "ATGCCAAGAGAAGGTTTAACCTTAAGATGTGAAAAATGTAAAATGGAAAATTACATTACAAAAAAGAACAAAAAATTACATCCAGACAAAATGGAAGTACAAAAATACTGCTCAAAATGTAATGCACATACAACACACAAAGAAAAGAAATAA", "aa": "MPREGLTLRCEKCKMENYITKKNKKLHPDKMEVQKYCSKCNAHTTHKEKK", "aa_hexdigest": "a94740e22d2064ea07f0b499858aa7c7", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC21A4", "ncbi_nrp_id": "WP_012855488.1", "uniref100_id": "UniRef100_D1J7Z8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21A4", "RefSeq:WP_012855488.1", "UniRef:UniRef100_D1J7Z8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7Z8", "uniref90_id": "UniRef90_B3PMG6" }, "psc": { "uniref90_id": "UniRef90_B3PMG6", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P35871", "cog_id": "COG0267", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P35871", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35871" ], "product": "Large ribosomal subunit protein bL33" }, "genes": [ "rpmG" ], "id": "AHCKFKLKGB_459", "locus": "GCA013367975_00435" }, { "type": "cds", "sequence": "contig_1", "start": 248779, "stop": 249189, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00557.29" ], "nt": "TTGGCTAAAAAGAAATATGCAACTGTTGGTGTTGAAAAGGACTATTTAATACTTGAAACATTTGAAAATTTAAAATCAATGTTGCCAAAATCTAAATTTGTGTTAGTAAATGGACAAGAATTTAGAATACTAAAAGATGAAGAAGAATATAGTAATGTTCAAGAAGCATGTAACATTTCTCTTCAATCTTTTGAACAAATAAAAACCGTTCTAACTGAAGGAATTACAGAATTAGAAGTTTCAAATAAATTAGGCTACTTAATGCGTTTATTTGGAGCTGAAAAAGAATCATTTGAAACAATTGTAGCTTTTGGTACAAATGCTGCTGAACCTCACCACCATCCAACAGATCGTAAACTTGCTGATGGTGATATTGTTAAGATAGACTTTGGTGCTCAATATCAAGGTTGA", "aa": "MAKKKYATVGVEKDYLILETFENLKSMLPKSKFVLVNGQEFRILKDEEEYSNVQEACNISLQSFEQIKTVLTEGITELEVSNKLGYLMRLFGAEKESFETIVAFGTNAAEPHHHPTDRKLADGDIVKIDFGAQYQG", "aa_hexdigest": "01e59e4a40b1bf253a9a3c195050a784", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00557.29", "name": "Peptidase_M24", "length": 87, "aa_cov": 0.625, "hmm_cov": 1.0, "evalue": 1.9730489926861978e-22, "score": 75.3111572265625, "start": 52, "stop": 136 } ], "seq_stats": { "molecular_weight": 15395.32599999998, "isoelectric_point": 5.111382865905762 }, "id": "AHCKFKLKGB_460", "locus": "GCA013367975_00436" }, { "type": "cds", "sequence": "contig_1", "start": 249466, "stop": 249591, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00557.29" ], "nt": "ATGATTATTACTGTTGAACCTGGTATATATATTGAAAACGAAGGTGGCGCTAGAATAGAAGACACTATTTTGGTTACAAAAGACGGCTCAAGAGTATTAAGCAGACCCGAAGATTATGAAAAATAA", "aa": "MIITVEPGIYIENEGGARIEDTILVTKDGSRVLSRPEDYEK", "aa_hexdigest": "c183ab7130d306e1a13ae1b24b5ee943", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00557.29", "name": "Peptidase_M24", "length": 28, "aa_cov": 0.6585365853658537, "hmm_cov": 1.0, "evalue": 9.935082387872104e-10, "score": 33.818260192871094, "start": 1, "stop": 27 } ], "seq_stats": { "molecular_weight": 4608.1418, "isoelectric_point": 4.442785835266113 }, "id": "AHCKFKLKGB_461", "locus": "GCA013367975_00437" }, { "type": "cds", "sequence": "contig_1", "start": 250186, "stop": 250464, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTATTTATTTCTGAATACCCTAGCAATTATCACACAAGTAAAATAACGCTAAAAGCGCGAAATAGAATTAGCGCAGCAATTTCTAATTATTTAGTCTTACTCTCTTGCTATAAAAGCACCATGAACAACTTAATAGATCAATTCTTAAATATTGGCAAAGAGGTTTATTGCCTTGATGATATTCAAAATAATTTGAATGAAGATAATTTCAACAAAAACTTAATTAATGAAGGAGCAGGAAAAATAACTTCAATAACTGATTTAATATTAAAATAG", "aa": "MLFISEYPSNYHTSKITLKARNRISAAISNYLVLLSCYKSTMNNLIDQFLNIGKEVYCLDDIQNNLNEDNFNKNLINEGAGKITSITDLILK", "aa_hexdigest": "833c2b3ed8d3b2711a41fee5a7c0f4ef", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10477.887899999987, "isoelectric_point": 6.536677360534668 }, "id": "AHCKFKLKGB_462", "locus": "GCA013367975_00438" }, { "type": "cds", "sequence": "contig_1", "start": 250456, "stop": 250701, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01261.30" ], "nt": "TTGCCTTTAATTAGAGTTATTCATTTAAATGATTCCATGAATGAACTTGGATCAAAAAAAGATCGTCATGCAAACATTGATAAAGGCCATATAGGTTTAGAAATATTGAAAAAATTTGTTCATGATAAAGACTTCGATAATATTCCTATTATTCTAGAGACACCGTTTATTAATAACAAAAGTCCTTACAAAGATGAAATAATAATGCTTTTAGAGGATAATAAAAAAGATAATATTCTATTTTAA", "aa": "MPLIRVIHLNDSMNELGSKKDRHANIDKGHIGLEILKKFVHDKDFDNIPIILETPFINNKSPYKDEIIMLLEDNKKDNILF", "aa_hexdigest": "b2f0103609553f00e778779e355aa3cb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387AC6AF", "query_cov": 0.8641975308641975, "subject_cov": 1.0144927536231885, "identity": 0.757, "score": 102.0, "evalue": 2.82e-27, "valid": false, "product": "TIM barrel protein", "uniref50_id": "UniRef50_Q49058" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01261.30", "name": "AP_endonuc_2", "length": 67, "aa_cov": 0.8148148148148148, "hmm_cov": 0.9850746268656716, "evalue": 6.686488821613535e-14, "score": 47.46160888671875, "start": 4, "stop": 69 } ], "seq_stats": { "molecular_weight": 9467.900699999991, "isoelectric_point": 6.0339365005493155 }, "id": "AHCKFKLKGB_463", "locus": "GCA013367975_00439" }, { "type": "cds", "sequence": "contig_1", "start": 250765, "stop": 251307, "strand": "-", "frame": 1, "gene": "nfo", "product": "deoxyribonuclease IV", "db_xrefs": [ "COG:COG0648", "COG:L", "EC:3.1.21.2", "GO:0003677", "GO:0006281", "GO:0008270", "SO:0001217", "UniRef:UniRef50_Q4A6S5", "UniRef:UniRef90_D1J801" ], "nt": "ATGATTAAATTAGGAAGCCATATATCGTTTAAAAAACCTGATTATTTAATAGGCTCATTTAATGAAGCTATTAATAATGGCGAAAATTGTTTTATGATTTATTTAGGAGCACCACAAAGTGCTTTTAGAGTTGATAGTTCATTATATAATATTGAAAAATATTTAAAGCAATGCAATAGTATTATTCCTGTATCTGATATTGTGGTTCATGCCCCATATATAGTAAATTTAGCCAATAGAGAAAAGGTAGATTTTGGGATTAAATTTTTATGTAGTGAAATTAAAAGAATGAATAGTTTTGGTGCTAAATTGTTAGTTTTACACCCAGGTTCATCAGTTGAATATAGCAGAAGCGAAGCATTAGCATCATTAATTCAATCTCTTAAAAAGATAATTGAATCTACTAAGGACGTAATAATATGCCTTGAAATAATGTCGGGTAAAGGCAGTCAAATTTGTAGCAATTTCAATGATTTATTATATGTGATAAAATCCGTTAATTCCGAAAGAATTGCAATATGTCTTGATACTTGCCATGCCTGA", "aa": "MIKLGSHISFKKPDYLIGSFNEAINNGENCFMIYLGAPQSAFRVDSSLYNIEKYLKQCNSIIPVSDIVVHAPYIVNLANREKVDFGIKFLCSEIKRMNSFGAKLLVLHPGSSVEYSRSEALASLIQSLKKIIESTKDVIICLEIMSGKGSQICSNFNDLLYVIKSVNSERIAICLDTCHA", "aa_hexdigest": "a8726765480384e3034f975b6381f773", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "nfo" ], "truncated": "5-prime", "pseudogene": { "start": 250462, "stop": 251307, "observations": { "mutation": [ 250767 ], "stop": [ 250767 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J801", "query_cov": 1.0, "subject_cov": 0.6360424028268551, "identity": 1.0, "score": 357.0, "evalue": 3.42e-123, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MIKLGSHISFKKPDYLIGSFNEAINNGENCFMIYLGAPQSAFRVDSSLYNIEKYLKQCNSIIPVSDIVVHAPYIVNLANREKVDFGIKFLCSEIKRMNSFGAKLLVLHPGSSVEYSRSEALASLIQSLKKIIESTKDVIICLEIMSGKGSQICSNFNDLLYVIKSVNSERIAICLDTCHAWDAGYDIKDYENFKSYIKQQNILPLIRVIHLNDSMNELGSKKDRHANIDKGHIGLETLKKFVHDKDFDNIPIILETPFINNKNPYKDEIIMLLEDNKKDNILF" }, "paralog": false, "identity": 0.9893238434163701, "score": 556.0, "evalue": 4.64e-204, "description": "Internal stop codon at 250767. Nonsense mutation around 250767.", "psc": { "uniref90_id": "UniRef90_D1J801", "gene": "Nfo", "product": "deoxyribonuclease IV", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_Q4A6S5", "cog_id": "COG0648", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J801", "EC:3.1.21.2", "UniRef:UniRef50_Q4A6S5", "COG:COG0648", "COG:L", "GO:0003677", "GO:0006281", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6S5" ], "product": "Probable endonuclease 4" } }, "id": "AHCKFKLKGB_464", "locus": "GCA013367975_00440" }, { "type": "cds", "sequence": "contig_1", "start": 251474, "stop": 251995, "strand": "-", "frame": 3, "gene": null, "product": "RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ5" ], "nt": "ATGGTTGAAACAATAAATAAAATATTGAAGATTGAACGTGAATTGCAACAAGAAAATGGATATCCTCCAACAGATGAAGAAATTGCTAAAAAATATGGTGGAGATTTTACTGCTGAAAAAGTAAGATATATTAGAAAAATTAATATCGATCCAATTAGTTTGGATAAAAACATTGGAAAAGAAGAAAATTCTAGTTTTTCAGATTTTGTTAAAGATGAAAGCGTAATCAGCCCAACTTCGTATGCAAGCCATGAAGAGTTAAGCGTTATTATAAATGAAATGATTGAAGAAGGGTTAAGTGATCAAGCTGATCGGGATTTAATTAGAAAACGTTTTGGAATTAACGACTTAAAGGGAAACAAATACAAACCTCATACTTTAGATGAACTTTCAAAAGAATTAGGCATTTCAAAAGAAAAAGTTAGACAATTAGAAACAAAAGTTTTGCGTAAATTAAAGCATCCTCAAAAACGTAGAAAATTGAAGGAATTCTTTAATAGCGATAGCTATGATTTAGATTAA", "aa": "MVETINKILKIERELQQENGYPPTDEEIAKKYGGDFTAEKVRYIRKINIDPISLDKNIGKEENSSFSDFVKDESVISPTSYASHEELSVIINEMIEEGLSDQADRDLIRKRFGINDLKGNKYKPHTLDELSKELGISKEKVRQLETKVLRKLKHPQKRRKLKEFFNSDSYDLD", "aa_hexdigest": "bb6960937ffe99faca464418640fb8db", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0024C90298", "query_cov": 0.9884393063583815, "subject_cov": 0.8142857142857143, "identity": 0.561, "score": 166.0, "evalue": 3.83e-49, "valid": false, "product": "sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0A448ZYJ5" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYJ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ5" ], "product": "RNA polymerase sigma factor" }, "genes": [], "id": "AHCKFKLKGB_465", "locus": "GCA013367975_00441" }, { "type": "cds", "sequence": "contig_1", "start": 252062, "stop": 252283, "strand": "-", "frame": 3, "gene": null, "product": "sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028FC70B1" ], "nt": "ATGCAAGAAGCTAAAGAAGCAAATAATTATTATAAATATAAAAAAGCTAGAGATATGTTAGTCAAAAGAAATCTCCGTTTAGTAATCAATAATGCAAAGCGTTATAAAAATCGTGGATTGAATTTTATTGATTTGATATCAGAAGGAAATGCTGGAATAATGAAGGCAGTTTCAAAATATGATTACACAAAAGGATTTAAATTTTCAACATATGCTACTTGA", "aa": "MQEAKEANNYYKYKKARDMLVKRNLRLVINNAKRYKNRGLNFIDLISEGNAGIMKAVSKYDYTKGFKFSTYAT", "aa_hexdigest": "51e3db9e296d577ba9aa1a44d51643eb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0020416EE7", "query_cov": 0.8493150684931506, "subject_cov": 0.8157894736842105, "identity": 0.629, "score": 80.5, "evalue": 1.71e-18, "valid": false, "product": "sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_UPI0028FC70B1" }, "pscc": { "uniref50_id": "UniRef50_UPI0028FC70B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028FC70B1" ], "product": "sigma-70 family RNA polymerase sigma factor" }, "genes": [], "id": "AHCKFKLKGB_466", "locus": "GCA013367975_00442" }, { "type": "cds", "sequence": "contig_1", "start": 252353, "stop": 253090, "strand": "-", "frame": 3, "gene": "rpoD", "product": "RNA polymerase sigma factor", "db_xrefs": [ "COG:COG0568", "COG:K", "SO:0001217", "UniRef:UniRef50_A0A501X9X8", "UniRef:UniRef90_D1J802" ], "nt": "ATGCAAGATACTAAAAATAATGTAGAATCATCAGTTAAGTGTATAACTAAAATTGTTGCAGACGAGTTAAAAAAAGTCCAAAAGAAAAATAAGGATAAAAAGGCTTTTACTCAAGATGAAATTTTTGAATTCCTAAACAAAAAGAACCTTTACGTTGATGACGAAGAAGCAGAAGCTTTACTTGAAAGCCTTTTTGCAGCTAATCTAGTCTCATCTGATACTGATGACGAAGATATTGAAGATATTGACATAAATTCGCTAGATGACGAAGTTGCTCATGCAACAACTAAAGATGATGATAATGATGACGATGACGCAGAAGAGGAAGAAAAAGATTTTGATCCTTCAAAAATTAAAGAGTTGGAAGAAGAAAATCTTGACGAAGATATAAATATTGATCAAGAATTTGACGATAGTTCAATTAGAAGCAAGGAAGAAGATGACTCAGAAATGTCTTTTGACGAAGATGATAATCATTTTGTTAAAAAGGCATTTAATGATCTTGATGATGAAGAATATAATGATTATTACGGCGAAGATTTTGACGAATATGAAGATGACGATTATCAAACAAACCACAAGCAAGGTAAAAAACAATCATACGATGACTTTGATGCTAACAATTTGCTAAAAGAATACAATGATGAAGATATAAAGTTGAGTGAAGAAAAAGTTTATGATCAAGATGATATAAAATTAACTAATAAACTAACCGAAACCAATGACATTGTTAAATGA", "aa": "MQDTKNNVESSVKCITKIVADELKKVQKKNKDKKAFTQDEIFEFLNKKNLYVDDEEAEALLESLFAANLVSSDTDDEDIEDIDINSLDDEVAHATTKDDDNDDDDAEEEEKDFDPSKIKELEEENLDEDINIDQEFDDSSIRSKEEDDSEMSFDEDDNHFVKKAFNDLDDEEYNDYYGEDFDEYEDDDYQTNHKQGKKQSYDDFDANNLLKEYNDEDIKLSEEKVYDQDDIKLTNKLTETNDIVK", "aa_hexdigest": "915da4e6aba3dd5b9385155b135b4b26", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "rpoD" ], "truncated": "5-prime", "pseudogene": { "start": 252050, "stop": 253090, "observations": { "mutation": [ 252061, 252064, 252343, 252355 ], "stop": [ 252061, 252064, 252343, 252355 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J802", "query_cov": 1.0163265306122449, "subject_cov": 0.45940959409594095, "identity": 0.94, "score": 372.0, "evalue": 2.62e-124, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 249, "reference_sequence": "MQDTKQDTKNNVESSVKCITKIVADELKKVQKKNKDKKAFTQDEIFEFLNKKNLYVDDEEAEALLESLFAANLVSSDTDDEDIEDIDINSLDDEVAHATTKDDDNDDDDAEEEEKDFDPSKIKELEEENLDEDINIDQEFDDSSIRSKEEDDSEMSFDEDDNHFVKKAFNDLDDEEYNDYYGEDFDEYEDDDYQTNHKQGKKQSYDDFDANNLLKEYNDEDIKLSEEKVYDQDDIKLTNKLTETNDIVKWYMRWIGKYGKLLTTNEEQALAKKMQEAKEANNYYKYKKARDMLVKRNLRLVINNAKRYKNRGLNFIDLISEGNAGIMKAVSKYDYTKGFKFSTYATWWIRQAITRAVADQARTVRVPVHMVETINKILKIERELQQENGYPPTDEEIAKKYGGDFTAEKVRYIRKINIDPISLDKNIGKEENSSFSDFVKDESVISPTSYASHEELSVIINEMIEEGLSDQADRDLIRKRFGINDLKGNKYKPHTLDELSKELGISKEKVRQLETKVLRKLKHPQKRRKLKEFFNSDSYDLD" }, "paralog": false, "identity": 0.9571428571428572, "score": 589.0, "evalue": 3.84e-212, "description": "Internal stop codon at 252061, 252064, 252343, 252355. Nonsense mutation around 252061, 252064, 252343, 252355.", "psc": { "uniref90_id": "UniRef90_D1J802", "gene": "RpoD", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0A501X9X8", "cog_id": "COG0568", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J802", "UniRef:UniRef50_A0A501X9X8", "COG:COG0568", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501X9X8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X9X8" ], "product": "RNA polymerase sigma factor" } }, "id": "AHCKFKLKGB_467", "locus": "GCA013367975_00443" }, { "type": "cds", "sequence": "contig_1", "start": 253150, "stop": 253380, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTTCCAAATGGATGTCAAAATCATAAAATGTTTTTGTGTTTTTTAATAAACAGCATAAAAAATAATAAATCAATAGATGAAACAAATTTTGAATCATATATTACAAACAATATTGATCTTGAAGATAATGCTAAACAAATATATTTAGATTTGTTCAAGGAAATTATGAGTCTTAAAAACAAAGAATATACAAAAGAAGAACTAGATAAAAATTTAAATAATTGA", "aa": "MAFPNGCQNHKMFLCFLINSIKNNKSIDETNFESYITNNIDLEDNAKQIYLDLFKEIMSLKNKEYTKEELDKNLNN", "aa_hexdigest": "b35b78f0069937f849c129fb0136152a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8998.102099999993, "isoelectric_point": 4.993385124206542 }, "id": "AHCKFKLKGB_468", "locus": "GCA013367975_00444" }, { "type": "cds", "sequence": "contig_1", "start": 253693, "stop": 254952, "strand": "-", "frame": 1, "gene": "dnaG", "product": "DNA primase", "db_xrefs": [ "COG:COG0358", "COG:L", "EC:2.7.7.101", "KEGG:K02316", "SO:0001217", "UniRef:UniRef50_D1J803", "UniRef:UniRef90_D1J803" ], "nt": "ATGGCAGAAGATAATATATTTGAAATTGTTAAACAAAAGTTAAACATTGTTGATGTTATTTCTTTATATATTTCTTTGACAAAAAAAGGAAAGAATTATGTGGCTTGTTGCCCATTTCATGGCGAAAAAACTCCTTCTTTTGTTGTGTCACAAGAAAAACAAATATTTAAGTGTTTTGGTTGCTCTAAAAGCGGAAATTTAATAACTTTCATTGAACTTTATACTAAAAAAAGCCCTCTTGAAAGTATCAAGGAGCTTGTTTCAAAATTCAATTTAAATATCGATTTAACAAGGTTTAGTAATAGTATAACTACTACAACAAAAGAACAAGAAGATTTATACAATATAAATCAAATAGCAAATGATATATTTCAATTTGAAATTTTAGAAATTAATCAAAATGAGCAATTAAAATTATTTCTTAAAAAAAGAAGGTTAACAAAACCACTTATTAAGGAATTTGAAATTGGCTTTGCTGATAAAGATAAAAATTTATGTAATATGCTAATTGCTAAAAATTGTTCATCATTTAATTTAGCCAATTCATCATTGATAGCAAGCAACAATTTTGATAACTTTTTTAATGATAGATTGATGTTTCCAATCAAAAATAAGGATGGAAGAATTGTTGCTTTTTCTGGAAGAAGTATTTATAATAATGTTGAACCAAAATATTTAAATAGTTCTGAAACAATAGTTTTTAAAAAGCATGAAGTTATGTTTAATTACTATCATGCATTGGATGAAATTATAAAGAAATCAAGTGTTTATTTAGTTGAGGGGCAATTTGATTGCATTGCTCTTTATAAAATAAACATAAAAAATGCAGTAGCACTCTTAGGCACGGCTTTATCTATACAAAATTTAGAATCTTTAAGAAATTGTAATATTGTTTTGTTTTTTGATAATGATTTTGCCGGCAAAAATGCTACTAAGAAAAATTTAAGAATAATATTAGCAAATCAAAAAAGATATAATTTAAAAGTTTCTTTTGTTTTGAATGAACATAGCAAAGATCCTGACGAATTATATAATATTGACGAAGGAAAAACATTGTCTGAGATAGCATGTTGTCCTGTCGATTTATTTATGTATTTATTCAATGAATTTAAACAAGTATTTCAATTGGAAACCAATGAATCAAGAAAGGTTGAATTAAATGCTGAATTGTTTGAATTTGTTATTTATTTGCAAGAACCATTAATATTGAAATTAAAAGAAAAATTGATTGAAAATAAAATTTTTTCAATTGAAACTTGA", "aa": "MAEDNIFEIVKQKLNIVDVISLYISLTKKGKNYVACCPFHGEKTPSFVVSQEKQIFKCFGCSKSGNLITFIELYTKKSPLESIKELVSKFNLNIDLTRFSNSITTTTKEQEDLYNINQIANDIFQFEILEINQNEQLKLFLKKRRLTKPLIKEFEIGFADKDKNLCNMLIAKNCSSFNLANSSLIASNNFDNFFNDRLMFPIKNKDGRIVAFSGRSIYNNVEPKYLNSSETIVFKKHEVMFNYYHALDEIIKKSSVYLVEGQFDCIALYKINIKNAVALLGTALSIQNLESLRNCNIVLFFDNDFAGKNATKKNLRIILANQKRYNLKVSFVLNEHSKDPDELYNIDEGKTLSEIACCPVDLFMYLFNEFKQVFQLETNESRKVELNAELFEFVIYLQEPLILKLKEKLIENKIFSIET", "aa_hexdigest": "7fc114e5b04fdbc2749a0a327458ce83", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dnaG" ], "truncated": "5-prime", "pseudogene": { "start": 253390, "stop": 254952, "observations": { "mutation": [ 253695 ], "stop": [ 253695 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J803", "query_cov": 1.0, "subject_cov": 0.6768982229402262, "identity": 0.998, "score": 813.0, "evalue": 5.5e-294, "gene_start": 1, "gene_end": 419, "reference_start": 1, "reference_end": 419, "reference_sequence": "MAEDNIFEIVKQKLNIVDVISLYISLTKKGKNYVACCPFHGEKTPSFVVSQEKQIFKCFGCSKSGNLITFIELYTKKSPLESIKELVSKFNLNIDLTRFSNSITTTTKEQEDLYNINQIANDIFQFEILEINQNEQLKLFLKKRRLTKPLIKEFEIGFADKDKNLCNMLIAKNCSSFNLANSSLIASNNFDNFFNDRLMFPIKNKDGRIVAFSGRSIYNNVEPKYLNSSETIVFKKHEVMFNYYHALDEIIKKSSVYLVEGQFDCIALYKINIKNAVALLGTALSIQNLESLRNCNIVLFFDNDFAGKNATKKNLRIILANQKRYNLKVSFVLNEHSKDPDELYNIDEGKTLSEIACCPVDLFMYLFNEFKQVFQLETNESRKVELNAELFEFVIYLQEPLILKLKEKLIENKIFSVETWNKYLFLYGKENFPLHPISKNNLKPSLDNEYNKKNYSKNSNYKKYEDLNFAYSDPSFDRLDFFEEPLIQQPKLITGRNLVFYLIIKTIIKHPQYIANYQLVDFARMAFSNGCQNHKMFLCFLINSIKNNKSIDETNFESYITNNIDLEDNTKQIYLDLFKEIMSLKNKEYTKEELDKNLNNWMKETGQSKDLIINLDRRK" }, "paralog": false, "identity": 0.9903846153846154, "score": 1027.0, "evalue": 0.0, "description": "Internal stop codon at 253695. Nonsense mutation around 253695.", "psc": { "uniref90_id": "UniRef90_D1J803", "gene": "DnaG", "product": "DNA primase", "ec_ids": [ "2.7.7.101" ], "uniref50_id": "UniRef50_D1J803", "cog_id": "COG0358", "cog_category": "L", "kegg_orthology_id": "K02316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J803", "EC:2.7.7.101", "UniRef:UniRef50_D1J803", "COG:COG0358", "COG:L", "KEGG:K02316" ] }, "pscc": { "uniref50_id": "UniRef50_D1J803", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J803" ], "product": "DNA primase" } }, "id": "AHCKFKLKGB_469", "locus": "GCA013367975_00445" }, { "type": "cds", "sequence": "contig_1", "start": 254963, "stop": 255481, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "TTGCTTGGTATTGCAAATCGTGGCAATTATGATTTAAGTCAACATATGAAATATTCAAATGAATCTCTTGAATATTTGTTAGACGATGGAACTAAAATAATTCCTCATGTTATTGAACCTTCAATTGGTTTAGATCGTTTATTGCTTGCGTTGCTTGTAGATTCATATGAAGAACAAGAACTAGAAAATAATGATGTAAGAATTGTTTTAAAACTTGATTTCAATATTGCACCATATCAAGTTGCGATTTTTCCGTTAATGAAAAAACTTAACTCTAAGGCTAAAGAAATATTTGATAATTTATTGCAAAAAACTGAATTAAGATTAATTTATGATGAAAGCGGTTCGATAGGAAAACGTTACCGTCGTCAAGATGCAATTGGTACACCATTTGCAGTAACAATAGATTTTGACACATTAGAAAAAAATACAGTAACTGTCAGAGAACGCGATTCTATGAAACAACATGTAGTTGAATTAGACAACTTAATTAAGTTTTTTGAAAATTATAAAAAATAA", "aa": "MLGIANRGNYDLSQHMKYSNESLEYLLDDGTKIIPHVIEPSIGLDRLLLALLVDSYEEQELENNDVRIVLKLDFNIAPYQVAIFPLMKKLNSKAKEIFDNLLQKTELRLIYDESGSIGKRYRRQDAIGTPFAVTIDFDTLEKNTVTVRERDSMKQHVVELDNLIKFFENYKK", "aa_hexdigest": "2442d64f90482f18560c9191389c5df0", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0P9T9", "query_cov": 0.9651162790697675, "subject_cov": 0.9707602339181286, "identity": 0.657, "score": 228.0, "evalue": 7.7e-74, "valid": false, "product": "Glycine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0P9T9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 93, "aa_cov": 0.5232558139534884, "hmm_cov": 1.0, "evalue": 1.1602747859933937e-23, "score": 78.49624633789062, "start": 80, "stop": 169 } ], "seq_stats": { "molecular_weight": 19984.670899999994, "isoelectric_point": 5.293097114562988 }, "id": "AHCKFKLKGB_470", "locus": "GCA013367975_00446" }, { "type": "cds", "sequence": "contig_1", "start": 255626, "stop": 255892, "strand": "-", "frame": 3, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "nt": "ATGTTTAAAACCCAAATGGGTGCAATTGATACAGCAGCAAATCAAACATATTTGCGTCCTGAAACAGCCCAAGGAATTTTTATAAATTTTAAAAATGTCTTAAGAACAACTAGAGCTAAGTTACCTTTTGGAATTGGCCAAATTGGTAAAAGTTTTAGAAATGAAATTACACCTAGGGATTTTATTTTTAGAACAAGGGAATTTGAACAAATGGAATTAGAATATTTCTGCAATCCTAGTGAAGATGAAAAGTTTTACCATTATTGA", "aa": "MFKTQMGAIDTAANQTYLRPETAQGIFINFKNVLRTTRAKLPFGIGQIGKSFRNEITPRDFIFRTREFEQMELEYFCNPSEDEKFYHY", "aa_hexdigest": "fb21550e42e24642fc3daa802e4e2712", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003241E146", "query_cov": 0.9318181818181818, "subject_cov": 0.8913043478260869, "identity": 0.695, "score": 123.0, "evalue": 5.97e-35, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_X1KVU4" }, "pscc": { "uniref50_id": "UniRef50_X1KVU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_471", "locus": "GCA013367975_00447" }, { "type": "cds", "sequence": "contig_1", "start": 256181, "stop": 256339, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGATAAAATACAAAAAAATATTCAAAATATAATAAATCACTTAAAAGTTTCTGGTTTTGTTTATCAAAATTCAGAAATCTATGGAGGCTTAATAAACACCTGGGATTATGGGCCATTAGCAGTTAATATTATGACTGCAATTAAGAAATATTGA", "aa": "MGDKIQKNIQNIINHLKVSGFVYQNSEIYGGLINTWDYGPLAVNIMTAIKKY", "aa_hexdigest": "060c8f592c19fc45fda4a41c098dc6d2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5915.793100000001, "isoelectric_point": 9.10114688873291 }, "id": "AHCKFKLKGB_472", "locus": "GCA013367975_00448" }, { "type": "cds", "sequence": "contig_1", "start": 256484, "stop": 257812, "strand": "+", "frame": 2, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "COG:COG0541", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501", "KEGG:K03106", "RefSeq:WP_175933452.1", "SO:0001217", "UniParc:UPI00158A97CA", "UniRef:UniRef100_UPI00158A97CA", "UniRef:UniRef50_A0A448ZXZ7", "UniRef:UniRef90_D1J805" ], "nt": "ATGTTAGATTTTATTCAAAAAAGAATGCAAAATTCAATACAAAAAATTAATAAAAAAATGTCTATTAATGAAGAAGACATTTTAGAAATTTTAAGAGAAATTAAAATATCGTTATTGGAGGCAGACGTTAACCTTGAAGTTGTTAAAAACTTCACTAAAGAAGTAAAGGCAAAAGCCCTTTCTTCTCAAATTATTGGAAAGTTAAATCAGCAACAAACAGTCCTAAAAATATTCAAAGATGAATTGACTAGAATTTTAGGTTCAAAATCAATTGATATTAAAACAAACAATAACCCTACTAAAATAATGATGGTAGGATTGCAAGGTTCAGGTAAAACTACTACTGCTGCAAAATTGTCTGTTTATTTCAAAAAGAAGAATTTGGCAGTAAACCCTTTGCTTGTTGGTGATGATATTTATAGACCTGCTGCCAGAGAACAATTGCAACAATTAGCAAAACAAACTCAAACAGATTTCTTTACCGCAGAAGAAAACAATGCAATTAATATTGCTCAAAAAGCAATAGAATTGGCAAAGGAAAACAAAAACGATTTAGTAATAATCGATACTGCCGGAAGATTGGCAATTGATGAAAATTTGATGCAAGAACTAAAAGATATTAAAAAATATACTCACCCTGATTATATATTTCTAGTAGTTGATGCAATGAGCGGACAAGATGTTATAAATACAGCAAAAATTTTCCACCAAGAACTTAATTTAGATGGCACTATTATTACAAAATTGGATAGTGATGCTCGTGGTGGCGCAGCTTTGTCGATTACTCATTTATTGAACGTTCCAATAATTTTTATTGGTACTGGTGAAAAATTAACTGGTTTAGAATTATTTCATCCAGATAGAATGGCAGATAGAATATTGGGCATGGGCGATGTTCTAACATTAATTGAAAAAGCTTCGGAAGAAGTTGATGAAAAAATGATGAAAAAAATTGGCTATAAAATGCTTTCTGGCCAATTCAATTTAATGGATTTGATGAATAGTTTAGCTCAAATTAAAAAACTAGGTAAGATGAAAGCAATCTTAAGAATGATTCCCGGAATGGCAGATAAGGTTTCAGACGAAAAGATTGAAGAAGCAGAAAATAAATTTAGAATTTATACTTATTTAATTAATTCAATGACGAACTTCGAGAAAAAAAACCCACGAGTTTTAAAAAACCCAAGTAGAAAGCAAAGAATTTTGCAAGGTTCTGGTAGAAGCCCAAGAGAATTTAATATGCTAATTTCGGATTTCGAAAGAATGTCTAAGCAAATGAAAGAATTAGCAAATGGAAATTTAAAATTAAAAAATGGCTTGGATGAATAA", "aa": "MLDFIQKRMQNSIQKINKKMSINEEDILEILREIKISLLEADVNLEVVKNFTKEVKAKALSSQIIGKLNQQQTVLKIFKDELTRILGSKSIDIKTNNNPTKIMMVGLQGSGKTTTAAKLSVYFKKKNLAVNPLLVGDDIYRPAAREQLQQLAKQTQTDFFTAEENNAINIAQKAIELAKENKNDLVIIDTAGRLAIDENLMQELKDIKKYTHPDYIFLVVDAMSGQDVINTAKIFHQELNLDGTIITKLDSDARGGAALSITHLLNVPIIFIGTGEKLTGLELFHPDRMADRILGMGDVLTLIEKASEEVDEKMMKKIGYKMLSGQFNLMDLMNSLAQIKKLGKMKAILRMIPGMADKVSDEKIEEAENKFRIYTYLINSMTNFEKKNPRVLKNPSRKQRILQGSGRSPREFNMLISDFERMSKQMKELANGNLKLKNGLDE", "aa_hexdigest": "938b6a71e82c79b3b4abcac7e768480f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158A97CA", "ncbi_nrp_id": "WP_175933452.1", "uniref100_id": "UniRef100_UPI00158A97CA", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158A97CA", "RefSeq:WP_175933452.1", "UniRef:UniRef100_UPI00158A97CA" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158A97CA", "uniref90_id": "UniRef90_D1J805" }, "psc": { "uniref90_id": "UniRef90_D1J805", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_A0A448ZXZ7", "cog_id": "COG0541", "cog_category": "U", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXZ7" ], "product": "Signal recognition particle protein" }, "genes": [ "ffh" ], "id": "AHCKFKLKGB_473", "locus": "GCA013367975_00449" }, { "type": "cds", "sequence": "contig_1", "start": 257922, "stop": 258113, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGCTTTTTATAATTTATTCTTATTTTTAATTGAAATTAAGACTAATATTGCTTTAGATATTTTTAAACTTGATAAAACATATCTATCAAAAATAATAATATTTCTTAATTTAAATATTGAATTTAATTTTTTAGTTTTTACAATTAGGTTATATGGAGAAATAGTTTTAATTTTATTGATGAAGTGA", "aa": "MGAFYNLFLFLIEIKTNIALDIFKLDKTYLSKIIIFLNLNIEFNFLVFTIRLYGEIVLILLMK", "aa_hexdigest": "9e5efd39970fa1f580ad824f38038867", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7493.086099999995, "isoelectric_point": 8.03393726348877 }, "id": "AHCKFKLKGB_474", "locus": "GCA013367975_00450" }, { "type": "cds", "sequence": "contig_1", "start": 258350, "stop": 258508, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTATTTTCATTCAACGATAATCAAAAATATATAGAAATTAAGAAATTTTTGTCTCAATTGAATACATTTAAGGAATTTATTTATTTTATAGTAGAAACACTTTTAAACTATAGCGATATTTATGCGAAATTAAAAACCTTTGATGAATTATGA", "aa": "MFLFSFNDNQKYIEIKKFLSQLNTFKEFIYFIVETLLNYSDIYAKLKTFDEL", "aa_hexdigest": "bf67931aa5b4ac998666b296d549052f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6415.358999999999, "isoelectric_point": 5.0959794998168935 }, "id": "AHCKFKLKGB_475", "locus": "GCA013367975_00451" }, { "type": "cds", "sequence": "contig_1", "start": 258653, "stop": 258817, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATGCATTTTTAAGCAACGAAACAAATAGCAAAGCTATATTTTTAACATTATTTAAAGATGGAAAAATTGATGATATATTATTTAGATTACAATCAAGACTTGAAGATGTATTATTAACTAAAAAAACAATTATTAATTTTGATTTGCGTGAACAATGA", "aa": "MIDAFLSNETNSKAIFLTLFKDGKIDDILFRLQSRLEDVLLTKKTIINFDLREQ", "aa_hexdigest": "ad6aca48d69c023ee65d932c650e4711", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6332.281099999998, "isoelectric_point": 5.125876808166503 }, "id": "AHCKFKLKGB_476", "locus": "GCA013367975_00452" }, { "type": "cds", "sequence": "contig_1", "start": 259253, "stop": 259606, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTTATAAATTAACTAATTTAATAACAGCAAAAAATGAAACAAAAACCAATTTAATTATTGAAATAAAAAAAGATTTTGAAAATATCGGCAAAGAAATTATTTCAAAATATCAAAAGCTTGTTGAATATATTCAAAAAATTAATCCTCTAGCAAATATCGTGGTAACAAATTATGCCAAACCATTTATGGCCTTACAAGATTTAATTAATTCGATTTACATTTCTTCTTCATATAACAATGAATTTGATTTGGTTGAAATGATACAAGATTTATTAAACAATGTCGCTAAAGAAGTAGCTAAAAAATGCCGATGTTTATATGTAGATATTTATGAAGAAAAATATTGA", "aa": "MPFYKLTNLITAKNETKTNLIIEIKKDFENIGKEIISKYQKLVEYIQKINPLANIVVTNYAKPFMALQDLINSIYISSSYNNEFDLVEMIQDLLNNVAKEVAKKCRCLYVDIYEEKY", "aa_hexdigest": "63979708af73f0b2cefc63473ac540e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13725.85089999999, "isoelectric_point": 6.29187183380127 }, "id": "AHCKFKLKGB_477", "locus": "GCA013367975_00453" }, { "type": "cds", "sequence": "contig_1", "start": 259703, "stop": 260068, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F5209ED", "UniRef:UniRef90_UPI001F5209ED" ], "nt": "ATGGCTAAAAATCAAAAATACAATTTAAATGACGAAATTAAATATGTTGCTTTAGGAGATTCATTTGCAGCGGGATTTAATAGCAAAGTAGGGTTTAATACTAATGGAAAATTATCAAATGGTAATATAGATGGTTTGGGTTATCCATCATTTTTAGCCTTAAAATTGCGAGATTTAAACTATAGATTAACAAGTTTTTACAATCTGGCAATACCAGCAGGTAATTTAGACATAATTTATGCCTTAACTTCAAATAATGAAAATGATTTAATTGCCAATTCAAAAAGTCTTGATTTGCTTCAATCAATTGATTGGCATGTTTCAAATCCTTCAAAGAATTTTTTTGATGAATTTTTTTTAAATTGA", "aa": "MAKNQKYNLNDEIKYVALGDSFAAGFNSKVGFNTNGKLSNGNIDGLGYPSFLALKLRDLNYRLTSFYNLAIPAGNLDIIYALTSNNENDLIANSKSLDLLQSIDWHVSNPSKNFFDEFFLN", "aa_hexdigest": "e9610aedb459f1cb65780fab95cbbf46", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001F5209ED", "query_cov": 0.9834710743801653, "subject_cov": 1.0, "identity": 0.992, "score": 238.0, "evalue": 9.53e-80, "valid": true, "uniref50_id": "UniRef50_UPI001F5209ED" }, "pscc": { "uniref50_id": "UniRef50_UPI001F5209ED", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F5209ED" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13508.941999999979, "isoelectric_point": 5.16594829559326 }, "id": "AHCKFKLKGB_478", "locus": "GCA013367975_00454" }, { "type": "cds", "sequence": "contig_1", "start": 260178, "stop": 260393, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAACTAAAAGATGAAGAAACCGGCGCCAAATTAGCTGTAGTTAGTTCAATAAAGTTGGAAGCTTTAAATATAAATGAAGTTATTGTTGCTACTTACCGCCTAATATCTAAGTATTATCCTCAAAAAATTATTGATATAAAATTGCAACCAAGTCAAGATGAAGAAGAAATCTTTAGCCAATTGAGTTTATATGCTAAAAATAATAATTGA", "aa": "MPKLKDEETGAKLAVVSSIKLEALNINEVIVATYRLISKYYPQKIIDIKLQPSQDEEEIFSQLSLYAKNNN", "aa_hexdigest": "8cd3b9a7b842bd0dcfd511df535b60af", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8098.235599999996, "isoelectric_point": 5.311001396179199 }, "id": "AHCKFKLKGB_479", "locus": "GCA013367975_00455" }, { "type": "cds", "sequence": "contig_1", "start": 260439, "stop": 260888, "strand": "-", "frame": 2, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_A0A077L649", "UniRef:UniRef90_A0A454CAF5" ], "nt": "ATGGCCAAAACCATTAGATTATTAAATGAAAAAATAAGATTATTTGATTTATTTATAATTGTTTTAGATGCACGCTTACCTCTTACTTCGTTTAATAACGATATTTATAAGATGGCTAATAATAAGCCAATTTTGTTCATATTAAACAAAAGCGACAAAGCAGATCTATCTAAATTAAAACCTTTTATAAATGAATATGAAAAGAAAGGTGTTGTTGTAACAACTAATTTAAAAGATAAATTAGCCTATAAAAAAATAAATTCAGCTCTTAATAATATTTATTTAAAAGTAAAAGAAAAAAATGCGCAAAAACATAAGCTAACACCAGCATTAAAATGTGTCGTGCTTGGCGTTCCTAATGTTGGTAAAAGTACCTTAATAAATTTATTGGCTGGTTCCAAAGTTGCAAAAGTGGGTGCAATGGCAGGAGTAACAAAGTCAGAGCAATGA", "aa": "MAKTIRLLNEKIRLFDLFIIVLDARLPLTSFNNDIYKMANNKPILFILNKSDKADLSKLKPFINEYEKKGVVVTTNLKDKLAYKKINSALNNIYLKVKEKNAQKHKLTPALKCVVLGVPNVGKSTLINLLAGSKVAKVGAMAGVTKSEQ", "aa_hexdigest": "5210e832a85ee2d2043f177ad2b5f1f3", "start_type": "ATG", "rbs_motif": null, "genes": [ "ylqF" ], "truncated": "both", "pseudogene": { "start": 260136, "stop": 260912, "observations": { "mutation": [ 260180, 260441, 260903 ], "stop": [ 260180, 260441, 260903 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CAF5", "query_cov": 1.0, "subject_cov": 0.5173611111111112, "identity": 0.993, "score": 278.0, "evalue": 2.49e-92, "gene_start": 1, "gene_end": 149, "reference_start": 9, "reference_end": 157, "reference_sequence": "MFNWFPGHMAKTIRLLNEKIRLFDLFIIILDARLPLTSFNNDIYKMANNKPILFILNKSDKADLSKLKPFINEYEKKGVVVTTNLKDKLAYKKINSALNNIYLKVKEKNAQKHKLTPALKCVVLGVPNVGKSTLINLLAGSKVAKVGAMAGVTKSEQWINCKNYLLLDTPGLLMPKLKDEETGAKLAVVSSIKLEALNINEVIVATYRLISKYYPQKIIDIKLQPSQDEEEIFSQLSLYAKNNNWILKNQLPDIKKAIVQLISYFNNLNNIVYDQCQEKEQNGWKSKI" }, "paralog": false, "identity": 0.9806201550387597, "score": 489.0, "evalue": 2.59e-178, "description": "Internal stop codon at 260180, 260441, 260903. Nonsense mutation around 260180, 260441, 260903.", "psc": { "uniref90_id": "UniRef90_A0A454CAF5", "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_A0A077L649", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CAF5", "UniRef:UniRef50_A0A077L649", "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L649", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L649" ], "product": "Ribosome biogenesis GTPase A" } }, "id": "AHCKFKLKGB_480", "locus": "GCA013367975_00456" }, { "type": "cds", "sequence": "contig_1", "start": 260921, "stop": 261136, "strand": "-", "frame": 3, "gene": "trmH", "product": "TrmH family RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "nt": "ATGTTTGGAACTGAAAGCACAGGAATTCCTAAGAGAATTATGCAAACAAATATTCCTAATTGTTTGCGTATTCCGATGTTTGATGAATGTCGTAGTTTGAATTTAGCTAATAGTGTTGCAATAGTACTATTTGAAGTATTAAGACAAAATGATTTCGCCGGACTATCAAAATTTGAAAGTCAAAAAGGGAAGGATTTTATTTTAAGGAAAGATTGA", "aa": "MFGTESTGIPKRIMQTNIPNCLRIPMFDECRSLNLANSVAIVLFEVLRQNDFAGLSKFESQKGKDFILRKD", "aa_hexdigest": "6bd8369e0e35ce00d282458f1ad0d93b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AH15", "query_cov": 1.0140845070422535, "subject_cov": 0.8674698795180723, "identity": 0.514, "score": 79.0, "evalue": 7.73e-18, "valid": false, "product": "TrmH family RNA methyltransferase", "uniref50_id": "UniRef50_A0A449AH15" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "product": "TrmH family RNA methyltransferase" }, "genes": [ "trmH" ], "id": "AHCKFKLKGB_481", "locus": "GCA013367975_00457" }, { "type": "cds", "sequence": "contig_1", "start": 261140, "stop": 261319, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAGGCCGCTGGACACTTTTTAACTGAAATTCAACATGAAGTTCATGAAAATTATGAAGAATTTTTTAAAAAATATGGTGATAAAAATATTTATTATGTAACAAGATACGGTTTAAAAAATTACGTAGATGTTGATTACAAACAAGAATTGCAAACAAATGGTGAACTTTGA", "aa": "MKRKAAGHFLTEIQHEVHENYEEFFKKYGDKNIYYVTRYGLKNYVDVDYKQELQTNGEL", "aa_hexdigest": "b02bb34b0d68b2a9b578926e87dc7e26", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7192.941000000004, "isoelectric_point": 6.26146297454834 }, "id": "AHCKFKLKGB_482", "locus": "GCA013367975_00458" }, { "type": "cds", "sequence": "contig_1", "start": 261713, "stop": 261979, "strand": "-", "frame": 3, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855499.1", "SO:0001217", "UniParc:UPI0001BC21E3", "UniRef:UniRef100_D1J809", "UniRef:UniRef50_P0C0K0", "UniRef:UniRef90_D1J809" ], "nt": "ATGGTATCAAAAGAAAAAAAAGCTGAATTAGTTGTTAAATTCGGACGTAATGCAAAAGACACTGGATATTTACCAGTACAAATAGCAATTTTAACTGAAGACATTGAAAGCTTAAAAGAACATTTTGCAGTAAATAAAAAAGATTTACATTCAATGAGAGGATTCATTGCTAAAGTTAATCACCGTAAAGCATTACTAAATCACTTAAAAGACGAAAACTACAACTTATATTTAGAAACCATCAAAGCTTTAAACATCAGAAAATAA", "aa": "MVSKEKKAELVVKFGRNAKDTGYLPVQIAILTEDIESLKEHFAVNKKDLHSMRGFIAKVNHRKALLNHLKDENYNLYLETIKALNIRK", "aa_hexdigest": "fbc082619cd1a86501261f37c1edf520", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC21E3", "ncbi_nrp_id": "WP_012855499.1", "uniref100_id": "UniRef100_D1J809", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21E3", "RefSeq:WP_012855499.1", "UniRef:UniRef100_D1J809" ] }, "ips": { "uniref100_id": "UniRef100_D1J809", "uniref90_id": "UniRef90_D1J809" }, "psc": { "uniref90_id": "UniRef90_D1J809", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P0C0K0", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P0C0K0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0C0K0" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "AHCKFKLKGB_483", "locus": "GCA013367975_00459" }, { "type": "tRNA", "sequence": "contig_1", "start": 262102, "stop": 262178, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 83.1, "nt": "GCGAGCGTAGCTCAGTTGGTTAGAGCACACGACTGATAATCGTGAGGTCGATGGTTCAAGTCCATTCGTTCGCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 262142, 262144 ], "id": "AHCKFKLKGB_31", "locus": "GCA013367975_00460" }, { "type": "cds", "sequence": "contig_1", "start": 262227, "stop": 262625, "strand": "-", "frame": 2, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02996", "RefSeq:WP_012855500.1", "SO:0001217", "UniParc:UPI0001BC21E4", "UniRef:UniRef100_D1J810", "UniRef:UniRef50_Q98Q72", "UniRef:UniRef90_D1J810" ], "nt": "ATGGCAGATAAAGTAAGATACTATGGTTTAGGTCGTCGTAAATCTTCAGTAGCAAGAGTTTATATTTTACCAGGTAGTGGAAATTTAATTATTAATGGAATAGTTGCAAAAGAATACTTAAATTCAGAAATTTTAATAAAAGATGCTTTAAGTCCTTTTGCAGTAACTGAAACTGCTAACCAATTTGATGTTTTTGCAAATGTTAATGGTGGTGGTTTAACAGGACAAGCTGGAGCAATTAGATTAGGTATTGCTAGAGCATTATTAGAAGCATCTAATAACGAATATAGAAATAAATTAAAAGATGCAGGATTTTTAACACGTGATGCTAGAGTTAAAGAACGTAAGAAATTCGGTTTAAGAAAAGCAAGACGTGCAAGACAATTTTCAAAACGTTAA", "aa": "MADKVRYYGLGRRKSSVARVYILPGSGNLIINGIVAKEYLNSEILIKDALSPFAVTETANQFDVFANVNGGGLTGQAGAIRLGIARALLEASNNEYRNKLKDAGFLTRDARVKERKKFGLRKARRARQFSKR", "aa_hexdigest": "8a2d74830272a058243860cc79a3c920", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC21E4", "ncbi_nrp_id": "WP_012855500.1", "uniref100_id": "UniRef100_D1J810", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21E4", "RefSeq:WP_012855500.1", "UniRef:UniRef100_D1J810" ] }, "ips": { "uniref100_id": "UniRef100_D1J810", "uniref90_id": "UniRef90_D1J810" }, "psc": { "uniref90_id": "UniRef90_D1J810", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q98Q72", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q72" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "AHCKFKLKGB_484", "locus": "GCA013367975_00461" }, { "type": "cds", "sequence": "contig_1", "start": 262628, "stop": 262912, "strand": "-", "frame": 3, "gene": null, "product": "50S ribosomal protein L13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "nt": "ATGGGCGACAATGTGGTTGTGATCAATGCTAAAGATGTACTTTTAACTGCTAAAAAAGATGAAATAAAAGTATATTACCACCACACTGGATACCCAGGTGGATTAAAACAAATTACCGCTGCTGATTTAAGAGCAAAAAAACCAGAATTATTAGTTGAAAAAGCTGTTAGAGGAATGCTTCCACACACCAAATTAGGTCGTAAACAATTTAAAAATTTATATGTTTATGCAGGTTCTGAACACAAACAAGAAGCACAACAACCAGTTAAATTAGAGGTTAAATAA", "aa": "MGDNVVVINAKDVLLTAKKDEIKVYYHHTGYPGGLKQITAADLRAKKPELLVEKAVRGMLPHTKLGRKQFKNLYVYAGSEHKQEAQQPVKLEVK", "aa_hexdigest": "5376e3427d2b3b0ced82ebaa8fcaf930", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P644", "query_cov": 1.0, "subject_cov": 0.831858407079646, "identity": 0.649, "score": 124.0, "evalue": 3.48e-35, "valid": false, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_A0A3B0P644", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "product": "50S ribosomal protein L13" }, "genes": [], "id": "AHCKFKLKGB_485", "locus": "GCA013367975_00462" }, { "type": "cds", "sequence": "contig_1", "start": 263175, "stop": 263837, "strand": "-", "frame": 2, "gene": "rsmE", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "db_xrefs": [ "COG:COG1385", "COG:J", "EC:2.1.1.193", "RefSeq:WP_175933454.1", "SO:0001217", "UniParc:UPI00158E2170", "UniRef:UniRef100_UPI00158E2170", "UniRef:UniRef50_A0A448ZY27", "UniRef:UniRef90_D1J812" ], "nt": "ATGTATAAATTTTTTGCCAACAAATTAGAAGATGGAATTTTTTATTTTAGTGATGAAATCCAAAAGCATTTTAAGGTTTTACGTTTTAACAAAGAAAATATTATTGTAAATTATGAAAATAAGTTTTATGAATGTCAATTTGTTTTTCCTAATAGTGCTAAATTAGTTAAAGAGTTAGATATTAATAATGAATTGGATTATAAAATTTCAGTTGCAATTCCTTTAATTAAACAAAACCATTTTGAAATAGCATTGCAAAAAGCAGTTGAACTTGGCGCTTATGAAATTTATATATTTCAAAGCCAATATGTTGATAAATCTAATTTAAATTTTGGTTCTAAAAAGCAACGTTATGAAAAAATAATTTTTGAAGCTTGCCAACAAAGTTTTAGAAATTATATTCCAAGACTTCATGATCCAATTTCATTTTCAGAACTTTGTGTCTTGGATATTAAAAATAAAGTATTAGCTTATGAAAATAAAAAAAATAACGAATTAATTAAAGATCGCCAAGATGCTTTAATTATTGTTGGCCCTGAAGGTGGATTTAGCCAACAAGAAATTGATTATGCCATATCTTGTAATGTTCAAGTTGTTTCACTAACAAAAACGATTTTAAGGGCTGAGACCGCCTTAATTTATATGCTTTCAAAATTAAATTAA", "aa": "MYKFFANKLEDGIFYFSDEIQKHFKVLRFNKENIIVNYENKFYECQFVFPNSAKLVKELDINNELDYKISVAIPLIKQNHFEIALQKAVELGAYEIYIFQSQYVDKSNLNFGSKKQRYEKIIFEACQQSFRNYIPRLHDPISFSELCVLDIKNKVLAYENKKNNELIKDRQDALIIVGPEGGFSQQEIDYAISCNVQVVSLTKTILRAETALIYMLSKLN", "aa_hexdigest": "bbee87012bee5d6171f20916b0c41571", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158E2170", "ncbi_nrp_id": "WP_175933454.1", "uniref100_id": "UniRef100_UPI00158E2170", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E2170", "RefSeq:WP_175933454.1", "UniRef:UniRef100_UPI00158E2170" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E2170", "uniref90_id": "UniRef90_D1J812" }, "psc": { "uniref90_id": "UniRef90_D1J812", "gene": "RsmE", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "ec_ids": [ "2.1.1.193" ], "uniref50_id": "UniRef50_A0A448ZY27", "cog_id": "COG1385", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZY27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZY27" ], "product": "Ribosomal RNA small subunit methyltransferase E" }, "genes": [ "rsmE" ], "id": "AHCKFKLKGB_486", "locus": "GCA013367975_00463" }, { "type": "cds", "sequence": "contig_1", "start": 264028, "stop": 264534, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "ATGATTTCAGATATTTCAATTAATTCAAATAATAAAAATTTTGAAATTAGAGACAATTTTTTTATTGATAAAAATAATAAAAAGATATTAGCATATTTGGATAAAAAGGTCACAAAAGTTACTATTCCTGGTTCAATAAAAGAAATTGGTGAAGTTGCATTTTCTGGTTGCGAAAACCTTAAGAAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGAGGCATTTAGAGTTAGGGCATTTTCAGATACAAACATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGCGAAAAAGCGTTTTATGATTGCGAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGAGGCATTTTATAATACAAAAATTGAATCTATTACTATTCCTAGTTCAATAAAAGGAATTGGTGAAGGTGCATTTTCTAGATGTGAAAACCTTAAAGAAGTAATTTATAAGGGCTATGCAAGTGAAATAAATTGA", "aa": "MISDISINSNNKNFEIRDNFFIDKNNKKILAYLDKKVTKVTIPGSIKEIGEVAFSGCENLKKVILNEGLEKIGAEAFRVRAFSDTNIESITIPGSVKEIGEKAFYDCENLKEVILNEGLEKIGAEAFYNTKIESITIPSSIKGIGEGAFSRCENLKEVIYKGYASEIN", "aa_hexdigest": "2b2d95bd858073fd8fa012859213ffa4", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 86, "aa_cov": 0.5119047619047619, "hmm_cov": 1.0, "evalue": 7.59976271637747e-40, "score": 131.09214782714844, "start": 80, "stop": 165 } ], "seq_stats": { "molecular_weight": 18653.098700000002, "isoelectric_point": 5.265018653869628 }, "id": "AHCKFKLKGB_487", "locus": "GCA013367975_00464" }, { "type": "cds", "sequence": "contig_1", "start": 264866, "stop": 265165, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTACCAAAAAATGCCCGAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCAACCAGAAAATGCACCTTCGCCGATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTACCACCAAATTCCCCAACGCCAAATGCATCTGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTCTACAACCAGAAAACGCTCATTGGCCAATTTCTTTTACTGAACTAGGAATAGTAATAGATTCAATATTTGTATCATAA", "aa": "MFVPKNARAPIFSRPSFKITSLRFSQPENAPSPISFTEPGIVIDSIFVPPNSPTPNASAPIFSRPSFKITSLRFLQPENAHWPISFTELGIVIDSIFVS", "aa_hexdigest": "4a8cc0888aa14954b933e7769fcb4ae0", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10945.473499999991, "isoelectric_point": 9.684714698791506 }, "id": "AHCKFKLKGB_488", "locus": "GCA013367975_00465" }, { "type": "cds", "sequence": "contig_1", "start": 265105, "stop": 265875, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05725.18", "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "TTGGATAAAAAGGCCACAAAAGTTACTATTCCTGGTTCAGCAAAAGAAATCGGCGAAGGTGCGTTTTCTGGTTGCGAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCAGAGGCATTTTATAATACAAAAATTGAATCTATTACTATTCCTGGTTCATTAAAAGAAATCGGCGAAGGCGCATTATCTAGTGGAAATATTTCAAGTATTTTAATTAATTCAAATAATAAAAATTTTGAAATTAAAGATAATTTTTTTATTGATAAAAATAATAAAAAGATATTAGCATATTTGGATAAAAAGGTCACAAAAGTTACTATTCCAAGCTCAGTAAAAGAAATTGACAAAGGTGCATTTTCTGGTTGCAAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGGTGCATTTTCAAGCACAAACATTGAATCTATTACTATTCCTGGTTCAATAAAAGAAATTGGTGAAGGTGCATTTTCTGGTTGCAAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGGTGCATTTTCAAGCACAAACATTGAATCTATTACTATTCCTGGTTCAATAAAAGAAATTGGTGAAGGTGCGTTTTCTGGTTGCGAAAACCTTAAGAAAGTAACTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGGTGCATTTTATGATACAAATATTGAATCTATTACTATTCCTAGTTCAGTAAAAGAAATTGGCCAATGA", "aa": "MDKKATKVTIPGSAKEIGEGAFSGCENLKEVILNEGLEKIGAEAFYNTKIESITIPGSLKEIGEGALSSGNISSILINSNNKNFEIKDNFFIDKNNKKILAYLDKKVTKVTIPSSVKEIDKGAFSGCKNLKEVILNEGLEKIGAGAFSSTNIESITIPGSIKEIGEGAFSGCKNLKEVILNEGLEKIGAGAFSSTNIESITIPGSIKEIGEGAFSGCENLKKVTLNEGLEKIGAGAFYDTNIESITIPSSVKEIGQ", "aa_hexdigest": "5940eb86dbdea9aac9cff3f3641b2d01", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05725.18", "name": "FNIP", "length": 36, "aa_cov": 0.140625, "hmm_cov": 1.0, "evalue": 1.7842569907685345e-07, "score": 26.578205108642578, "start": 219, "stop": 254 }, { "id": "PF13306.12", "name": "LRR_5", "length": 110, "aa_cov": 0.4296875, "hmm_cov": 1.0, "evalue": 2.3526038404826612e-76, "score": 249.10057067871094, "start": 102, "stop": 211 }, { "id": "PF13855.12", "name": "LRR_8", "length": 35, "aa_cov": 0.1328125, "hmm_cov": 1.0, "evalue": 3.3280246091563637e-13, "score": 44.56373596191406, "start": 116, "stop": 149 } ], "seq_stats": { "molecular_weight": 27059.52890000001, "isoelectric_point": 5.177316093444824 }, "id": "AHCKFKLKGB_489", "locus": "GCA013367975_00466" }, { "type": "cds", "sequence": "contig_1", "start": 266164, "stop": 266751, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "ATGATTTCAGATATTTCAATTAATTCAAATAATAAAAATTTTGAAATTGAAGATAACTTTTTTATTGACAAAAATAATAAAAAAATATTATCATATTTGGATAAAAAGGCCACAAAAGTTACTATTCCTAGTTCAATAAAAGAAATTGGTGAAGGTGCATTTTCTGGTTGCGAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGTGCTGAGGCATTTTATAAAACAAAAATTGAATCTATTACTATTCTTGGTTCAATAAAAGAAATTGGTGAAGGTGCATTTTCTGGTTGCGAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGAGGCATTTTATAAAACAAAAATTGAATCTATTACTATTCCTGGTTCAATAAAAGAAATTGGCGCAAGTGCATTTTCAAGCACAAACATTGAATCTATTACTATTCCTAGTTCAGTAAAAGAAATTGGTGAAGGTGCGTTTTTTGGTTGCGAACACCTTACAAGAGCATTTTTAAATGAAGGTCTTGAAAAAATTGGCGCTCGGGCATTTAGTAGAAGTACAAACATTTGA", "aa": "MISDISINSNNKNFEIEDNFFIDKNNKKILSYLDKKATKVTIPSSIKEIGEGAFSGCENLKEVILNEGLEKIGAEAFYKTKIESITILGSIKEIGEGAFSGCENLKEVILNEGLEKIGAEAFYKTKIESITIPGSIKEIGASAFSSTNIESITIPSSVKEIGEGAFFGCEHLTRAFLNEGLEKIGARAFSRSTNI", "aa_hexdigest": "ebe7b628c16b5270a5b8d36564c77856", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 112, "aa_cov": 0.5743589743589743, "hmm_cov": 1.0, "evalue": 2.70997625674804e-51, "score": 168.0948486328125, "start": 35, "stop": 146 }, { "id": "PF13855.12", "name": "LRR_8", "length": 34, "aa_cov": 0.16923076923076924, "hmm_cov": 1.0, "evalue": 2.3067749070916935e-08, "score": 29.05915641784668, "start": 45, "stop": 77 } ], "seq_stats": { "molecular_weight": 21199.875900000006, "isoelectric_point": 5.079325675964354 }, "id": "AHCKFKLKGB_490", "locus": "GCA013367975_00467" }, { "type": "cds", "sequence": "contig_1", "start": 266771, "stop": 267577, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTATTTCTTTTACTGTATCAGGAATAGTAATAGATTTAATGTTTGTATTTGAAAATGCCCGAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGATTTTGCAATCAGAAAACGCACCTTCACCAATTTCTTTTACTGAACTAGGAATAGTAACTTTTGTGGCCTTTTTATCCAAATATGATAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATCTGAAATCATTGAAAAATCAAATGATCAATTGCCTATTTCTTTTACTGTATCAGGAATAGTAATAGATTTAATGTTTGTACGATCAAATGCCCGAGCGCCAATTTTTTCAAGACCTTCATTTAAAGTTACTTCTTTAAGGTTTTCACATCTAAAAAATGCACCAGCGCCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTTTATCAGCAAATGCACTTTCGCCAATTTTTTCAAGACCTTCATTTAAAGTTACTTTCTTAAGGTTTTTGCAACCAGAAAATGTACTTTTGTCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTGCTTGAAAATGCACCAGCGCCAATTTTTTCAAGACCTTTATTTAAAATTACTTCTTCAAGATTTTCACAACCAGAAAACGCACTTTCACCAATTTCTTTTACTGTACCAGGAATAGTAATAGATTCAATGTTTGTATCAGAAAATGCCCAAGCGCCAATTTCTGTAATATTTTTGCCTTGA", "aa": "MPISFTVSGIVIDLMFVFENARAPIFSRPSFKITSLRILQSENAPSPISFTELGIVTFVAFLSKYDNIFLLFLSIKKLSLISKFLLFELIEISEIIEKSNDQLPISFTVSGIVIDLMFVRSNARAPIFSRPSFKVTSLRFSHLKNAPAPISFIEPGIVIDSIFLSANALSPIFSRPSFKVTFLRFLQPENVLLSISFIEPGIVIDSMFVLENAPAPIFSRPLFKITSSRFSQPENALSPISFTVPGIVIDSMFVSENAQAPISVIFLP", "aa_hexdigest": "418d80dcfd6da2dff3023710dba698de", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29777.692200000016, "isoelectric_point": 7.989067268371582 }, "id": "AHCKFKLKGB_491", "locus": "GCA013367975_00468" }, { "type": "cds", "sequence": "contig_1", "start": 267631, "stop": 267909, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCCATTAGCAACTATTTCTTGTGATTATGGAGTAAAACAAAATTCAAAAGAAAACAAAGAGGATTTAAAACCCGATGAAAATAAACCAGAACCTAAACCAGAACCTAAACCAGAACCTAAACCCGAACCTAAACCAGAACCTAAACCAGAACCTAAACCAGAACCTAAACCCGAACCTAAACCCGAACCTAAACCAGAAAATCCTACCAAAGAAGAAGATTCATCAAATTTAAATAAAACTTTTAAATTTGAACTAACTAAATCAAATACTTGA", "aa": "MLPLATISCDYGVKQNSKENKEDLKPDENKPEPKPEPKPEPKPEPKPEPKPEPKPEPKPEPKPEPKPENPTKEEDSSNLNKTFKFELTKSNT", "aa_hexdigest": "aa7f64c7b6814dae60dad1e3218ddcb5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10453.643599999992, "isoelectric_point": 5.343570137023924 }, "id": "AHCKFKLKGB_492", "locus": "GCA013367975_00469" }, { "type": "cds", "sequence": "contig_1", "start": 268243, "stop": 268554, "strand": "-", "frame": 1, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "COG:COG0206", "COG:D", "GO:0000910", "GO:0003924", "GO:0005525", "RefSeq:WP_241857910.1", "SO:0001217", "UniParc:UPI001F5176D0", "UniRef:UniRef100_Q8RT91", "UniRef:UniRef50_A0A3L9ZYL3", "UniRef:UniRef90_D1J814" ], "nt": "ATGAGGGAAATTGTTGGTAATGAAATTAACATTATTTTTGGTATAACAACTCTAGAATCTGAAGAAAGCAACAAATTAGGTGAGCTATTTGTCTCGGTAATCGCCACGGGATTAAGAAAAGACGCTCCTAAAGACATTGATCAAATTCAAGATGAAGTAATCAATGTAATAAAAAAAGACGATTTAAATTATGTTAACGATGAAACAAAGGAATTCTTTGTATCTGAAGGAACATTTAAAACACAATCATTTTTTTCAATGGATAACGATGAAGATTCAAACGATGAACTTGCAGATATATTAAAACACTAA", "aa": "MREIVGNEINIIFGITTLESEESNKLGELFVSVIATGLRKDAPKDIDQIQDEVINVIKKDDLNYVNDETKEFFVSEGTFKTQSFFSMDNDEDSNDELADILKH", "aa_hexdigest": "e84d90497158d66f58330e865028e1b9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001F5176D0", "ncbi_nrp_id": "WP_241857910.1", "uniref100_id": "UniRef100_Q8RT91", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F5176D0", "RefSeq:WP_241857910.1", "UniRef:UniRef100_Q8RT91" ] }, "ips": { "uniref100_id": "UniRef100_Q8RT91", "uniref90_id": "UniRef90_D1J814" }, "psc": { "uniref90_id": "UniRef90_D1J814", "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_A0A3L9ZYL3", "cog_id": "COG0206", "cog_category": "D", "go_ids": [ "GO:0000910", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3L9ZYL3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3L9ZYL3" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "AHCKFKLKGB_493", "locus": "GCA013367975_00470" }, { "type": "cds", "sequence": "contig_1", "start": 268820, "stop": 269206, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "ATGGCCACAAAAGTTACTATTCCTGGTTCAGTAAAAGAAATTGGCGAAAGTGCGTTTTCTAGTTGCAGAAACCTTGAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTTGGGCATTTTATGATACAAACATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGCGAAAGTACATTTTCTGGTTGCGAAAACCTTGAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGAGGCATTTGAATATACAAAAATTGAATCTATTACTATTCCTGATACAGTAAAAGAAATTCGCAAAGGTGCGTTTTATAATTGCAAAAACCTTAAAGAAGTAATTTATAAAGGTGATGCAAGTAATATAAATTGA", "aa": "MATKVTIPGSVKEIGESAFSSCRNLEEVILNEGLEKIGAWAFYDTNIESITIPGSVKEIGESTFSGCENLEEVILNEGLEKIGAEAFEYTKIESITIPDTVKEIRKGAFYNCKNLKEVIYKGDASNIN", "aa_hexdigest": "bbd7a6db1570c4af8081ab9abf6bde3e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 114, "aa_cov": 0.890625, "hmm_cov": 1.0, "evalue": 3.2544655082796206e-41, "score": 135.51495361328125, "start": 3, "stop": 116 } ], "seq_stats": { "molecular_weight": 14044.70909999998, "isoelectric_point": 4.567945289611817 }, "id": "AHCKFKLKGB_494", "locus": "GCA013367975_00471" }, { "type": "cds", "sequence": "contig_1", "start": 269241, "stop": 269717, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAATAAAAAAGTTATCTTCAATTTCAAAATTTTTATTATTTGAATTAATTAAAATACTTGAAATATTTCCACTAGATAATGCACCTTCGCCGATTTCTTTTAATGAACCAGGAATAGTAATAGATTCAATTTTTGTATCAAAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTCAAGGCTATGGCAACCAGAAAATGTACTTTTACCAATTTCTTTTACTGAACCAGGAACAGTAATAGATTTAATGTTTGTATTTGAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTTATTTAAAATTACTTTCTTAAGGTTTTTGCAACCAAGAAATGTACTTTTGTCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTATTGCTAAATGCCCAAGCGCCGATTTTTTCAAGACCTTCATTTAAAACTACTTTTTTAAGGTCATAG", "aa": "MSIKKLSSISKFLLFELIKILEIFPLDNAPSPISFNEPGIVIDSIFVSKNASAPIFSRPSFKITSSRLWQPENVLLPISFTEPGTVIDLMFVFENASAPIFSRPLFKITFLRFLQPRNVLLSISFIEPGIVIDSMFVLLNAQAPIFSRPSFKTTFLRS", "aa_hexdigest": "e2655232c02dd1931fc7e44687839522", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17800.835099999986, "isoelectric_point": 9.769039344787597 }, "id": "AHCKFKLKGB_495", "locus": "GCA013367975_00472" }, { "type": "cds", "sequence": "contig_1", "start": 269868, "stop": 270038, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTGCAATTTTTCAAGTATTTGATTTAGTTAGTTCAAATTTAAAACTTTTATTTAAATTTGATGGATCTTCTTCTTTGGTAGGATTTTCTGGTTTAGGATTTGGTTTATTTTCATCAGGCTTTGAATCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTTTATGA", "aa": "MNFAIFQVFDLVSSNLKLLFKFDGSSSLVGFSGLGFGLFSSGFESSLFSFEFCFTL", "aa_hexdigest": "2ed5f44c066960b4a57b7ffbd2c2a77c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6168.004299999995, "isoelectric_point": 4.316091728210449 }, "id": "AHCKFKLKGB_496", "locus": "GCA013367975_00473" }, { "type": "cds", "sequence": "contig_1", "start": 270706, "stop": 272082, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21717.2", "PFAM:PF05725.18", "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "TTGGATAAAAAAACCACAAAAGTTACTATTCCTGGTTCAGTAAAAGAAATTGGTGAAGGTGCGTTTTATGGTTGCACAGGCCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCCAGGGCATTTTATGATACAAAAATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGACAAAAATACATTTTCTGGTTGCCCAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATCGGCGCTGAGACATTTAGAGATACAAAAATTGAATCTATTACTATTCCTAGTTCAGTAAAAGAAATTGACAAAAATACATTTTCTGGTTGCCCAAACCTTAAAAAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGAGACATTTAGAGATACAAAAATTGAATCTATTACTATTCCTGGTTCAATAAAAGAAATTGGCGAAAGTGCGTTTTCTGGTTGCCCAAACCTTAAAAAAGTAATTTTAAATGAAGGTCTTGAAAAAATCGGCGATTATGCATTTTATGATACAAAAATTGAATCTATTACTATTCCTGGCACAGTAAAAGAAATTGGCGAAAGTGCGTTTTGTAATTGCAAAAACCTTAAAGAAGTAATTTTAAATAAAGGTCTCGAAAAAATTGGCGCTTGGGCATTTGATGGTACAAAAATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGCAAAAGAGCGTTTTCTGATTGCAAAAACCTTAAGAAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGAGGCATTTTCAAAGACAAACATCAAATCTATTACTATTCCTGGTTCAGTAAAAGAATTTGGCGATTATGCGTTTTCTGATTGCAAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATCGGCGCTTGGGCATTTGATGGTACAAACATTGAATCTATTTCTATTCCTGGTTCAGTAACAGAAATTGGCGTTGGTGGATTTGATTTTTCAACAATTTTGCATATTTCAATTAATTCAAATAATAAAAATTTTGAAATTAGAGACAACTTTTTTATTGATAAAAATAATAAAAAGATATTAGCATATTTGGATAAAAAGGTCACAAAAGTTACTATTCCTGGTTCAGTAACAGAAATTGGCAGAGGTTCGTTTTCTAATTGCCAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATCGGCGCTTGGGCATTTAATCAAACAAAAATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGCTTAGGTACATTTTCTGGTTGTATAAAACTTAAAGAAGTAATTTATAAAGGTGATGCAAGTAAAATAGATTGA", "aa": "MDKKTTKVTIPGSVKEIGEGAFYGCTGLKEVILNEGLEKIGARAFYDTKIESITIPGSVKEIDKNTFSGCPNLKEVILNEGLEKIGAETFRDTKIESITIPSSVKEIDKNTFSGCPNLKKVILNEGLEKIGAETFRDTKIESITIPGSIKEIGESAFSGCPNLKKVILNEGLEKIGDYAFYDTKIESITIPGTVKEIGESAFCNCKNLKEVILNKGLEKIGAWAFDGTKIESITIPGSVKEIGKRAFSDCKNLKKVILNEGLEKIGAEAFSKTNIKSITIPGSVKEFGDYAFSDCKNLKEVILNEGLEKIGAWAFDGTNIESISIPGSVTEIGVGGFDFSTILHISINSNNKNFEIRDNFFIDKNNKKILAYLDKKVTKVTIPGSVTEIGRGSFSNCQNLKEVILNEGLEKIGAWAFNQTKIESITIPGSVKEIGLGTFSGCIKLKEVIYKGDASKID", "aa_hexdigest": "39630a89fa222a9e231732db0d478296", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21717.2", "name": "DTX3L_a-b", "length": 33, "aa_cov": 0.07205240174672489, "hmm_cov": 0.9696969696969697, "evalue": 1.937994643889769e-09, "score": 32.670501708984375, "start": 105, "stop": 137 }, { "id": "PF05725.18", "name": "FNIP", "length": 24, "aa_cov": 0.05240174672489083, "hmm_cov": 0.9583333333333334, "evalue": 1.824372635610242e-07, "score": 26.54729652404785, "start": 415, "stop": 438 }, { "id": "PF13306.12", "name": "LRR_5", "length": 126, "aa_cov": 0.27510917030567683, "hmm_cov": 1.0, "evalue": 7.894392532364628e-161, "score": 522.1456298828125, "start": 174, "stop": 299 }, { "id": "PF13855.12", "name": "LRR_8", "length": 29, "aa_cov": 0.0611353711790393, "hmm_cov": 1.0, "evalue": 2.849347223890131e-16, "score": 54.38839340209961, "start": 148, "stop": 175 } ], "seq_stats": { "molecular_weight": 49804.684399999984, "isoelectric_point": 5.966923332214355 }, "id": "AHCKFKLKGB_497", "locus": "GCA013367975_00474" }, { "type": "cds", "sequence": "contig_1", "start": 272179, "stop": 273078, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05725.18", "PFAM:PF13306.12", "PFAM:PF13855.12", "PFAM:PF23952.1" ], "nt": "TTGGATAAAAAGGTCACAAAAGTTACTATTCCTAGTTCAATAAAAGAAATTGGTGAAGGTGCGTTTTATGGTTGCACAGGCCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTAGCGCCAGGGCATTTTATGATACAAAAATTGAATCTATTACTATTCCTAGTTCAGTAAAAGAAATTGACCAAAATACATTTTCTGGTTACCCAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATCGGCGCTGAGGCATTTGAATATGCAAACATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGCAAAGGTGCGTTTTCTGGTTGCAAAAACCTTAAAGAAGTAATTTTAAATAAAGGTCTCGAAAAAATTGGCGCTTGGGCATTTTATAATACAAAAATTGAATCTATTACTATTCCTGGCACAGTAAAAGAAATTGACAAAAATACATTGTCTGGTTGCAAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTAGGGCATTTAGCGTTGAGGCATTTGAAGATACAAACATTGAATCTATTACTATTCCTAGTTCAGTAAAAGAAATTGGTGAAGGTGCGTTTTATGGTTGCGCAGGCCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGTTGGGGCATTTTATAATACAAAAATTGAATCTATTACTATTCCTGGCACAGTAAAAGAAATTGACAAAAATACATTGTCTGGTTGTGAAAACCTTAAGAAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGAGGCATTTTCAAAGACAAACATCAAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGCGAAGGCGCATTATCTAGTTGA", "aa": "MDKKVTKVTIPSSIKEIGEGAFYGCTGLKEVILNEGLEKISARAFYDTKIESITIPSSVKEIDQNTFSGYPNLKEVILNEGLEKIGAEAFEYANIESITIPGSVKEIGKGAFSGCKNLKEVILNKGLEKIGAWAFYNTKIESITIPGTVKEIDKNTLSGCKNLKEVILNEGLEKIGARAFSVEAFEDTNIESITIPSSVKEIGEGAFYGCAGLKEVILNEGLEKIGVGAFYNTKIESITIPGTVKEIDKNTLSGCENLKKVILNEGLEKIGAEAFSKTNIKSITIPGSVKEIGEGALSS", "aa_hexdigest": "c87ab2035ed8dc2de9410f9f8fa0650b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05725.18", "name": "FNIP", "length": 31, "aa_cov": 0.10367892976588629, "hmm_cov": 1.0, "evalue": 1.8160506821948264e-09, "score": 32.955501556396484, "start": 217, "stop": 247 }, { "id": "PF13306.12", "name": "LRR_5", "length": 115, "aa_cov": 0.38461538461538464, "hmm_cov": 1.0, "evalue": 4.885008110135556e-99, "score": 322.41754150390625, "start": 183, "stop": 297 }, { "id": "PF13855.12", "name": "LRR_8", "length": 37, "aa_cov": 0.12040133779264214, "hmm_cov": 1.0, "evalue": 1.3891013478032349e-12, "score": 42.576194763183594, "start": 13, "stop": 48 }, { "id": "PF23952.1", "name": "LRR_EndoS", "length": 59, "aa_cov": 0.1806020066889632, "hmm_cov": 1.0, "evalue": 7.641524266751518e-08, "score": 27.939546585083008, "start": 139, "stop": 192 } ], "seq_stats": { "molecular_weight": 32113.477300000028, "isoelectric_point": 5.1199655532836905 }, "id": "AHCKFKLKGB_498", "locus": "GCA013367975_00475" }, { "type": "cds", "sequence": "contig_1", "start": 273175, "stop": 273408, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "TTGGCATTTAGCGCTGGGCCATTTACTCGCACAAAAATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGTGAAGGTGCGTTTTATGGTTGCACAGGCCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATCGGCGCTGGGGCATTTTGTGATACAAAAATTGAATCTATTACTATTCCTGGTTCAATAAAAGAAATTGGCGAAGGCGCATTATCTAGTTGA", "aa": "MAFSAGPFTRTKIESITIPGSVKEIGEGAFYGCTGLKEVILNEGLEKIGAGAFCDTKIESITIPGSIKEIGEGALSS", "aa_hexdigest": "f86440393a7e4cee79d8dec5913de634", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 70, "aa_cov": 0.9090909090909091, "hmm_cov": 1.0, "evalue": 2.6149953455805064e-21, "score": 71.17628479003906, "start": 7, "stop": 76 } ], "seq_stats": { "molecular_weight": 7938.020099999996, "isoelectric_point": 4.795187568664551 }, "id": "AHCKFKLKGB_499", "locus": "GCA013367975_00476" }, { "type": "cds", "sequence": "contig_1", "start": 274083, "stop": 274472, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_104940430.1", "SO:0001217", "UniParc:UPI000CD67A4D", "UniRef:UniRef100_UPI000CD67A4D", "UniRef:UniRef50_UPI002FE2A79D", "UniRef:UniRef90_UPI000CD67A4D" ], "nt": "ATGTATAAATTTTTTGCAAACAAATTAGAAGATGGAATTTTTTATTTTAGTGATGAAATCCAAAGTGCTTTGGGAGCTATGCGGGAAATTGTTGGTAATGAAATTAACATTATTTTTGGTATAACAACTCTAGAATCTGAAGAAAGCAACAAATTAGGTGAGCTATTTGTCTCGGTAATCGCCACGGGATTAAGAAAAGACGCTCCCAAAGACATTGATCAAATTCAAGACGAAGTAATCAATGTAATAAAAAAAGACGATTTAAATTATGTTAACGATGAAACAAAGGAATTCTTTGTATCTGAAGGAACATTTAAAACACAATCATTTTTTTCAATGGATAATGATGAAGATTCAAACGATGAACTTGCAGATATATTAAAACACTAA", "aa": "MYKFFANKLEDGIFYFSDEIQSALGAMREIVGNEINIIFGITTLESEESNKLGELFVSVIATGLRKDAPKDIDQIQDEVINVIKKDDLNYVNDETKEFFVSEGTFKTQSFFSMDNDEDSNDELADILKH", "aa_hexdigest": "e872aa7fb3edc83ea3e9f7ba95dc9313", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000CD67A4D", "ncbi_nrp_id": "WP_104940430.1", "uniref100_id": "UniRef100_UPI000CD67A4D", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CD67A4D", "RefSeq:WP_104940430.1", "UniRef:UniRef100_UPI000CD67A4D" ] }, "ips": { "uniref100_id": "UniRef100_UPI000CD67A4D", "uniref90_id": "UniRef90_UPI000CD67A4D" }, "psc": { "uniref90_id": "UniRef90_UPI000CD67A4D", "uniref50_id": "UniRef50_UPI002FE2A79D" }, "pscc": { "uniref50_id": "UniRef50_UPI002FE2A79D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE2A79D" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14706.173199999987, "isoelectric_point": 4.195479393005371 }, "id": "AHCKFKLKGB_500", "locus": "GCA013367975_00477" }, { "type": "cds", "sequence": "contig_1", "start": 274933, "stop": 275097, "strand": "-", "frame": 1, "gene": null, "product": "leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000967FFA2" ], "nt": "TTGAATAAAGGTCTTGAAAAAATTGGCGCTTGGGCATTTAGCGATACAAAAATTGAATCTATTACTATTCCCGGTTCAGTAAAAGAAATTGGCGAAGATGCGTTTAATGGTTGCACAAACCTTAAAGAAGTAATTTTAAATGAAGGCCTTGAAGGAATTGGCTGA", "aa": "MNKGLEKIGAWAFSDTKIESITIPGSVKEIGEDAFNGCTNLKEVILNEGLEGIG", "aa_hexdigest": "3553dd5f103de537f0d64b84ba4ef789", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI000DCB5A76", "query_cov": 0.8703703703703703, "subject_cov": 0.8867924528301887, "identity": 0.809, "score": 76.3, "evalue": 2.08e-17, "valid": false, "product": "leucine-rich repeat protein", "uniref50_id": "UniRef50_UPI000967FFA2" }, "pscc": { "uniref50_id": "UniRef50_UPI000967FFA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000967FFA2" ], "product": "leucine-rich repeat domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_501", "locus": "GCA013367975_00478" }, { "type": "cds", "sequence": "contig_1", "start": 275177, "stop": 275665, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCTTTTCATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCGAAATTTTTATTATTTGAATTAATTGAAATATGTGAAATTCTTGAAAAATCAAATGCCCTGACGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGTATCTAAAAATGCCTTAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTGTGCAACCAGAAAATGCACTTTCGCCAATTTCTTTTACTGTATCAGGAATAGTAATAGATTCAATGTTTGTATATGAAAATGCACAAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTCTTAAGGTTTGCGCAATCAGAAAACGCACCTGCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGGATTATTAATATTAATTAACCCAGCAAATGCCGAATAG", "aa": "MAFSSKYANIFLLFLSIKKLSLISKFLLFELIEICEILEKSNALTPISFTEPGIVIDSMFVSKNALAPIFSRPSFKITSLRFVQPENALSPISFTVSGIVIDSMFVYENAQAPIFSRPSFKITFLRFAQSENAPAPISFTEPGIVIDSIFGLLILINPANAE", "aa_hexdigest": "7eff1a3c3f8df27d897fff58965f991b", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17933.86319999998, "isoelectric_point": 5.517838478088377 }, "id": "AHCKFKLKGB_502", "locus": "GCA013367975_00479" }, { "type": "cds", "sequence": "contig_1", "start": 275728, "stop": 276153, "strand": "-", "frame": 1, "gene": null, "product": "leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015EC9057" ], "nt": "ATGGCCACAAAAGTTACTATTCCTAGTTCAATAAAAGAAATTGGCGAAGGTGCGTTTTTAGGCCACGGAGACCTTCATGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTTGGGCATTTGATTTTTCAATAATTTCACATATTTCAATTAATTCAAATAATAAAAATTTTGAAATTAAAGATAACTTTTTTATTGATAAAAATAATAAAAAGATATTAGCATATTTGGATAAAAAGGCCACAAAAGTTACTATTCCTGGATCAGTAAAAGAAATTGATGAAAGAGCGTTTTCTTGGTGCCCAAACCTTAAAGAAGTAATTTTAAATAAAGGTCTTGAAAAAATTGGTGCTGAGGCATTTTATAAAACAAAAATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGCGAATGA", "aa": "MATKVTIPSSIKEIGEGAFLGHGDLHEVILNEGLEKIGAWAFDFSIISHISINSNNKNFEIKDNFFIDKNNKKILAYLDKKATKVTIPGSVKEIDERAFSWCPNLKEVILNKGLEKIGAEAFYKTKIESITIPGSVKEIGE", "aa_hexdigest": "f65acbea3fb96bf6d614c4d86366fb7b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0015EC9057", "query_cov": 0.8865248226950354, "subject_cov": 0.8333333333333334, "identity": 0.68, "score": 163.0, "evalue": 4.2e-49, "valid": false, "product": "leucine-rich repeat domain-containing protein", "uniref50_id": "UniRef50_UPI0015EC9057" }, "pscc": { "uniref50_id": "UniRef50_UPI0015EC9057", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015EC9057" ], "product": "leucine-rich repeat domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_503", "locus": "GCA013367975_00480" }, { "type": "cds", "sequence": "contig_1", "start": 276154, "stop": 276258, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTACACTTATTTTAATTAATTCAAATAATAAAAATTTTGAAATTAAAGATAACTTTTTTATTGATAAAAATAATAAAAAAATATTATCATATTTGGATTGA", "aa": "MTTLILINSNNKNFEIKDNFFIDKNNKKILSYLD", "aa_hexdigest": "1c1839a3b324874c461d2a7e0cdb724d", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4062.642600000001, "isoelectric_point": 8.099308204650882 }, "id": "AHCKFKLKGB_504", "locus": "GCA013367975_00481" }, { "type": "cds", "sequence": "contig_1", "start": 276274, "stop": 276834, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "ATGGCCACAAAAGTTACTATTCCTAGTTCAATAAAAGAAATTGGTGAAGGTGCATTTTCTGGTTGCGAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGGGGCATTTCATTTTTCAGACATTTCACATATTTCAATTAATTCAAATAATAAAAATTTTGAAATTGAAGATAACTTTTTTATTGACAAAAATAATAAAAAAATATTATCATATTTGGATAAAAAGGCCACAAAAGTTACTATTCCTAGTTCAGTAAAAGAAATTGGTGAAGGTGCATTTTCTGGTTGCGAAAACCTTAAAGAAGTAACTTTAAATGAAGGTCTTGAAAAAATTGGTGCTGAGGCATTTTATAAAACAAACATTGAATCTATTACTATTCCTAGTTCAGTAAAAGAAATTGGCGAAAGAGCGTTTCATGGTTGCACAAACCTTAATGAAGCAATTTTAAATGAAGGTCTTGAAAAAATTGGCGTTGAAGCATTTAGATATACAAAAATTGGAATTATTACTATTCCTGGTTCAGTAAAGGAAATTGGCAATTGA", "aa": "MATKVTIPSSIKEIGEGAFSGCENLKEVILNEGLEKIGAGAFHFSDISHISINSNNKNFEIEDNFFIDKNNKKILSYLDKKATKVTIPSSVKEIGEGAFSGCENLKEVTLNEGLEKIGAEAFYKTNIESITIPSSVKEIGERAFHGCTNLNEAILNEGLEKIGVEAFRYTKIGIITIPGSVKEIGN", "aa_hexdigest": "e72c3f6ad840ebd0e883ad3a7a005e30", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 107, "aa_cov": 0.5752688172043011, "hmm_cov": 1.0, "evalue": 3.353442251886039e-50, "score": 164.56349182128906, "start": 80, "stop": 186 }, { "id": "PF13855.12", "name": "LRR_8", "length": 48, "aa_cov": 0.24731182795698925, "hmm_cov": 1.0, "evalue": 9.433017824572138e-09, "score": 30.303010940551758, "start": 10, "stop": 55 } ], "seq_stats": { "molecular_weight": 20215.735000000004, "isoelectric_point": 5.239043235778809 }, "id": "AHCKFKLKGB_505", "locus": "GCA013367975_00482" }, { "type": "cds", "sequence": "contig_1", "start": 276869, "stop": 277591, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAATAAAAAAGTTTTCTTCAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATCTGAAATCATTGAAAAATCAAATGCTCAATTGCCTATTTCTTTTACTGTATCAGGAATAGTAATAGATTTAATGTTTGTATTTGAAAATGCCCGAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGATTTTGCAATCAAAAAACGCACTTTCGCCAATTTCTTTTACTGAACTAGGAATAGTAACTTTTGTGGCCTTTTTATCCAAATATGATAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATCTGAAATCATTGAAAAATCAAATGATCAATTGCCTATTTCTTTTACTGTATCAGGAATAGTAATAGATTTAATGTTTGTATTTGAAAATGCCCGAGCGCCAATTTTTTCAAGACCTTTATTTAAAATTACTTCTTCAAGATTTTCACAATCACAAAACGCACTTTCGCCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTTTATCAGCAAATGCACTTTCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTCTTAAGGTTTTCGCAACCAGAAAATGTACTTTTGTCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTTTTATAA", "aa": "MSIKKFSSISKFLLFELIEISEIIEKSNAQLPISFTVSGIVIDLMFVFENARAPIFSRPSFKITSLRILQSKNALSPISFTELGIVTFVAFLSKYDNIFLLFLSIKKLSLISKFLLFELIEISEIIEKSNDQLPISFTVSGIVIDLMFVFENARAPIFSRPLFKITSSRFSQSQNALSPISFIEPGIVIDSIFLSANALSPIFSRPSFKITFLRFSQPENVLLSISFIEPGIVIDSMFVL", "aa_hexdigest": "c89b321a8113402ef55108e079f87c99", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27003.571000000007, "isoelectric_point": 7.9135103225708 }, "id": "AHCKFKLKGB_506", "locus": "GCA013367975_00483" }, { "type": "cds", "sequence": "contig_1", "start": 277805, "stop": 277996, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_217702318.1", "SO:0001217", "UniParc:UPI001C37BB16", "UniRef:UniRef100_UPI001C37BB16", "UniRef:UniRef50_UPI001C37BB16", "UniRef:UniRef90_UPI001C37BB16" ], "nt": "TTGTCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTGCTTGAAAATGCACCAGCGCCAATTTTTTCAAGACCTTTATTTAAAATTACTTCTTCAAGATTTTCACAATCACAAAACGCACTTTCGCCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTGTATCATAA", "aa": "MSISFIEPGIVIDSMFVLENAPAPIFSRPLFKITSSRFSQSQNALSPISFIEPGIVIDSIFVS", "aa_hexdigest": "e94eb37218678e3fb56b304e44b9e2a3", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001C37BB16", "ncbi_nrp_id": "WP_217702318.1", "uniref100_id": "UniRef100_UPI001C37BB16", "db_xrefs": [ "SO:0001217", "UniParc:UPI001C37BB16", "RefSeq:WP_217702318.1", "UniRef:UniRef100_UPI001C37BB16" ] }, "ips": { "uniref100_id": "UniRef100_UPI001C37BB16", "uniref90_id": "UniRef90_UPI001C37BB16" }, "psc": { "uniref90_id": "UniRef90_UPI001C37BB16", "uniref50_id": "UniRef50_UPI001C37BB16" }, "pscc": { "uniref50_id": "UniRef50_UPI001C37BB16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001C37BB16" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6889.937399999998, "isoelectric_point": 4.512527275085449 }, "id": "AHCKFKLKGB_507", "locus": "GCA013367975_00484" }, { "type": "cds", "sequence": "contig_1", "start": 278153, "stop": 278314, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAGGATTTTCTGGTTTAGGTTCTGGTTTAGGTTCGGGTTTAGGTTCTGGTTTAGGTTCGGGTTTAGGTTCTGGTTTAGGTTCTGGTTTAGGTTCGGGTTTATTTTCATCGGGTTTTAAATCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTCCATAA", "aa": "MVGFSGLGSGLGSGLGSGLGSGLGSGLGSGLGSGLFSSGFKSSLFSFEFCFTP", "aa_hexdigest": "9f4076d6878d3a01b5d7f6b2036337c3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4957.502599999998, "isoelectric_point": 5.750594139099121 }, "id": "AHCKFKLKGB_508", "locus": "GCA013367975_00485" }, { "type": "cds", "sequence": "contig_1", "start": 278677, "stop": 279822, "strand": "-", "frame": 1, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "COG:COG0206", "COG:D", "GO:0000910", "GO:0003924", "GO:0005525", "RefSeq:WP_175933459.1", "SO:0001217", "UniParc:UPI00158BB1E2", "UniRef:UniRef100_UPI00158BB1E2", "UniRef:UniRef50_A0A3L9ZYL3", "UniRef:UniRef90_D1J814" ], "nt": "ATGGCAAAAGAATTAAATAATTATAACCCGGTGGCAAAAATAATTGTTATTGGAGTAGGTGGTGGTGGAAATAATAGTGTTGAAACTATGATAAATAGCCATTTAGATAGTTTTCAAATTATTGCTGCCAATACCGATAAACAAGTATTAACAAAATTTCCTCAAGAATGCGTTTTACATTTAGGTGATGAACGTGGAATTGGCGCGGGTGCTAACCCTGAAATCGGCAAAACTGCTGCTGAATCTTCGCGTGAAGAAATCAAAAGTAGATTGCAAGGAGCTGATCTAGTAATTATAACTGCAGGAATGGGTGGTGGAACCGGCACAGGTGCAGCGCCCGTGATTGCACAAATAGCCAAGGAATGCAATGCTTTAGTAGTTGCGGTTGTAACTACTCCGTTTGACTTTGAAGGTCCAAAGCGAATGAGAATTGCAAAGCAAGGCTTACAAGAAATTAAAAAATGTGTTGATTCATATATTGTTATTTCAAACAATAAACTATTACAACAATATGGAAATATTTCTTTTTCAGATGCATTTATTTGTGCAAATAATGTCTTAAAACAAACTATTAGAACAATAGTTGATGTTATTGCCACCCCAAGCATTATTAATCTTGATTTTGCAGATTTATCAACCATTATTAAAAATAAAGGTGAAACAGTAATCGGCATTGGCCAAGCAAATGGCCAAGATCGGGCTGTAAAAGCAATCACATCTGCAATAACAAGCCCAATTTTAGAATCAAGTGTTGTTGGGGCTAGTGATGCAATAGTAAATTTCAGTGCTTCTCAAAAGGTAACTTTAAATGAAATTCAAAGTGCTTTGGGAGCTATGAGGGAAATTGTTGGTAATGAAATTAACATTATTTTTGGTATAACAACTCTAGAATCTGAAGAAAGCAACAAATTAGGTGAGCTATTTGTCTCGGTAATCGCCACGGGATTAAGAAAAGACGCTCCCAAAGACATTGATCAAATTCAAGATGAAGTAATCAATGTAATAAAAAAAGACGATTTAAATTATGTTAACGATGAAACAAAGGAATTCTTTGTATCTGAAGGAACATTTAAAACACAATCATTTTTTTCAATGGATAATGATGAAGATTCAAACGATGAACTTGCAGATATATTAAAACACTAA", "aa": "MAKELNNYNPVAKIIVIGVGGGGNNSVETMINSHLDSFQIIAANTDKQVLTKFPQECVLHLGDERGIGAGANPEIGKTAAESSREEIKSRLQGADLVIITAGMGGGTGTGAAPVIAQIAKECNALVVAVVTTPFDFEGPKRMRIAKQGLQEIKKCVDSYIVISNNKLLQQYGNISFSDAFICANNVLKQTIRTIVDVIATPSIINLDFADLSTIIKNKGETVIGIGQANGQDRAVKAITSAITSPILESSVVGASDAIVNFSASQKVTLNEIQSALGAMREIVGNEINIIFGITTLESEESNKLGELFVSVIATGLRKDAPKDIDQIQDEVINVIKKDDLNYVNDETKEFFVSEGTFKTQSFFSMDNDEDSNDELADILKH", "aa_hexdigest": "248d2d5e42360acd0a0d77b969495716", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158BB1E2", "ncbi_nrp_id": "WP_175933459.1", "uniref100_id": "UniRef100_UPI00158BB1E2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158BB1E2", "RefSeq:WP_175933459.1", "UniRef:UniRef100_UPI00158BB1E2" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158BB1E2", "uniref90_id": "UniRef90_D1J814" }, "psc": { "uniref90_id": "UniRef90_D1J814", "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_A0A3L9ZYL3", "cog_id": "COG0206", "cog_category": "D", "go_ids": [ "GO:0000910", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3L9ZYL3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3L9ZYL3" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "AHCKFKLKGB_509", "locus": "GCA013367975_00486" }, { "type": "cds", "sequence": "contig_1", "start": 279830, "stop": 279970, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCAATAGATAATATTTTAGAATGTCAAATCAAGCAAAATGCTTTAAATAATGAAATTAGTAGGGAATTATTTACAATTGAAGTTTATAGTAATTCCAAAAAGTACTACAATTTAATGCTAAACGATATAAATTAG", "aa": "MLAIDNILECQIKQNALNNEISRELFTIEVYSNSKKYYNLMLNDIN", "aa_hexdigest": "18d5c39f4a1dba767c77377ba2b8f4bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5438.170000000001, "isoelectric_point": 4.655875205993653 }, "id": "AHCKFKLKGB_510", "locus": "GCA013367975_00487" }, { "type": "cds", "sequence": "contig_1", "start": 280229, "stop": 280564, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTAAACTAGTTAAAAAAACTAATATTAAGCAAGTTTATTCTTTAAAAACAATATCAAATGCTAATAGTCTAAGATTGCAAGAAATATTTAGTAGCAACGATTTAAAAATTGCCAAAATATTTTGCTTAGATAGTTTAAAGACAAATTCAGTTTTGTTTAAAAATTCAAAACAAAAAGTAGCTATTTTTGTTGATGTATTAGATGATAAAGCTAAATTTATTTTAAACATTAATAAATCAAATATAGTAAGCCAAGTATTAGATTTAGGACTAAACAAATTAAAAGCAAGAATGTTACAATGTAATTTATTAAGTACAGAAAGCAATTGA", "aa": "MEFKLVKKTNIKQVYSLKTISNANSLRLQEIFSSNDLKIAKIFCLDSLKTNSVLFKNSKQKVAIFVDVLDDKAKFILNINKSNIVSQVLDLGLNKLKARMLQCNLLSTESN", "aa_hexdigest": "4a69b394779e7889bd7591567e80179f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12580.710499999983, "isoelectric_point": 9.76794338226318 }, "id": "AHCKFKLKGB_511", "locus": "GCA013367975_00488" }, { "type": "cds", "sequence": "contig_1", "start": 280664, "stop": 280897, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E400CB7", "UniRef:UniRef90_UPI001E400CB7" ], "nt": "ATGAAAAAAATTATTATTGCTGCATATAAATTTTTAAAAAATAGTATCGAAGTAGAAGTTATGGAAAAAACTAATACTTTATCTTTTTGCATTTATAAAAAAGCAATTAATAATATTTATTTAACTGAAAGCGATATAAAAAATTTTATAAAGCAAACAAAGAATGAAATTCAAAAATTAATATCAGCAAGCATAAATGAAGTTATTGTTATGTTGTCTGAAGATAGCAAATGA", "aa": "MKKIIIAAYKFLKNSIEVEVMEKTNTLSFCIYKKAINNIYLTESDIKNFIKQTKNEIQKLISASINEVIVMLSEDSK", "aa_hexdigest": "29c293c4bbf54aa26858a8c72d03686c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 280576, "stop": 280897, "observations": { "insertion": [ 280606 ], "mutation": [ 280666 ], "stop": [ 280666 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E400CB7", "query_cov": 1.0, "subject_cov": 0.7264150943396226, "identity": 1.0, "score": 147.0, "evalue": 2.03e-44, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MKKIIIAAYKFLKNSIEVEVMEKTNTLSFCIYKKAINNIYLTESDIKNFIKQTKNEIQKLISASINEVIVMLSEDSKWNINSKLLTLNNDNFNISNLKILDQTSII" }, "paralog": false, "identity": 0.9905660377358491, "score": 194.0, "evalue": 1.73e-65, "description": "Internal stop codon at 280666. Frameshift due to insertion around 280606. Nonsense mutation around 280666.", "psc": { "uniref90_id": "UniRef90_UPI001E400CB7", "uniref50_id": "UniRef50_UPI001E400CB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E400CB7", "UniRef:UniRef50_UPI001E400CB7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E400CB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E400CB7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8904.433299999999, "isoelectric_point": 9.04112682342529 }, "id": "AHCKFKLKGB_512", "locus": "GCA013367975_00489" }, { "type": "cds", "sequence": "contig_1", "start": 280998, "stop": 281804, "strand": "-", "frame": 2, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0016434", "SO:0001217", "UniRef:UniRef50_Q6KHR2", "UniRef:UniRef90_D1J816" ], "nt": "ATGGAACATATTCCAGTTCTATTAAATGAAGTAATAGAACAATTATCAATAAAACCAGACGGAATATATGTTGATTTAACATTAGGCCGTGGTGGTCATAGCCAAGAAATTTTAAAAAGACTAACAACTGGAAAATTAATTTGTTTTGATAAAGATATTCAAGCTATAAACGAAAGTAGAGAAAAATTAATAAAAATAAGCCAAAACTTTATTTTAATAAAAAGTGATTTTAGAAACATTACTTGTGAACTAAAAAAACTAAATATTGATAAAGTCGATGGAATATTAGCAGATTTAGGAGTTTCTAGTCCTCAATTAGATGATCCAAATAGAGGATTTTCTTATTCAATCAATTCTGATTTAGATATGAGAATGGATTTAGATAATACTCTAACTGCAAAAGAAATTATTAATAATGCAACTGAAAAAGAAATTATAGATATATTGCAATCTAATGCTGATGTAAAATTAAGTAATTTAATTGCGAAAGCTATTGTAAAAAATCGACCAATATCTGATTCATTTACGTTAAATCAAATAATAAAGGATTCATTGCCAGCTAAAATAGTAAGACAAAAAAATCCTTCTAAAGCAATTTTTCAAGCTTTTAGAATAGCAGTGAATGATGAACTAAATGCCTTAAGTGAAATGCTAAGCCAACTTCCTAATTTATTAAAAAAAGACGGAAAAATAGCGATTATAACATTTCATAGTAAAGAAGACGTTATAGTTAAAAAGTTTTATCAAAATTTAAATTATTTTGACCCTAAGTTAAAAAAAATACTAATTGTTGAAACTCATAAGTGA", "aa": "MEHIPVLLNEVIEQLSIKPDGIYVDLTLGRGGHSQEILKRLTTGKLICFDKDIQAINESREKLIKISQNFILIKSDFRNITCELKKLNIDKVDGILADLGVSSPQLDDPNRGFSYSINSDLDMRMDLDNTLTAKEIINNATEKEIIDILQSNADVKLSNLIAKAIVKNRPISDSFTLNQIIKDSLPAKIVRQKNPSKAIFQAFRIAVNDELNALSEMLSQLPNLLKKDGKIAIITFHSKEDVIVKKFYQNLNYFDPKLKKILIVETHK", "aa_hexdigest": "36ff3b59f11e9e4bf538d7067d084b58", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_D1J816", "query_cov": 1.0, "subject_cov": 0.8933333333333333, "identity": 0.9890000000000001, "score": 509.0, "evalue": 1.06e-181, "valid": true, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_Q6KHR2", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0016434" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR2" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "AHCKFKLKGB_513", "locus": "GCA013367975_00490" }, { "type": "cds", "sequence": "contig_1", "start": 281805, "stop": 282245, "strand": "-", "frame": 2, "gene": "mraZ", "product": "Transcriptional regulator MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "RefSeq:WP_012855507.1", "SO:0001217", "UniParc:UPI0001BC21EB", "UniRef:UniRef100_D1J817", "UniRef:UniRef50_Q6KHR3", "UniRef:UniRef90_D1J817" ], "nt": "ATGTTTGGTAAATATTATCGCCAAATTGATGATAAAAATAGAGTTGTTGTTCCGCCTAAATTACTACAAGAATTAGGCAAAGAATTCTATATCACAATTGGAATAGATAAAAACTTAGTATTAAGAACGGTTTCTGAGTTTCAAAAAATGAAAACTAAATTAGAAGAAGGAAACACACTTAACAAAAACGTAAGAGATTTTATTCGCTACATTTTTGGAAATACTGAAGTTGTATCTCCTGATAAAAATAACAGAATCATGATTCCCAAACATCTTTTAAATAAAGCCGCTATCGACAAAGAAGTTGTTTTCCTAGGAACTGGTGATACTTGTGAATTATTTGCTAAAGAAGTTTATGATGAACAAGAAAGCTACTATGAAAATGATGATAATGTAAATGACTTGGCTCAAAAATTATTTGAAGCAGGAGTTAAGTTCTAA", "aa": "MFGKYYRQIDDKNRVVVPPKLLQELGKEFYITIGIDKNLVLRTVSEFQKMKTKLEEGNTLNKNVRDFIRYIFGNTEVVSPDKNNRIMIPKHLLNKAAIDKEVVFLGTGDTCELFAKEVYDEQESYYENDDNVNDLAQKLFEAGVKF", "aa_hexdigest": "90a8ff3ff46d05845bdb9147a418c90f", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001BC21EB", "ncbi_nrp_id": "WP_012855507.1", "uniref100_id": "UniRef100_D1J817", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21EB", "RefSeq:WP_012855507.1", "UniRef:UniRef100_D1J817" ] }, "ips": { "uniref100_id": "UniRef100_D1J817", "uniref90_id": "UniRef90_D1J817" }, "psc": { "uniref90_id": "UniRef90_D1J817", "gene": "MraZ", "product": "Transcriptional regulator MraZ", "uniref50_id": "UniRef50_Q6KHR3", "cog_id": "COG2001", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_Q6KHR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR3" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "AHCKFKLKGB_514", "locus": "GCA013367975_00491" }, { "type": "tRNA", "sequence": "contig_1", "start": 282435, "stop": 282510, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 79.9, "nt": "GCTAGTTTAGCTCAGTTGGTAGAGCAATTGATTCGTAATCAATGGGTCGGGGGTTCGATCCCTCTAACTAGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 282468, 282470 ], "id": "AHCKFKLKGB_16", "locus": "GCA013367975_00492" }, { "type": "tRNA", "sequence": "contig_1", "start": 282601, "stop": 282675, "strand": "+", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 77.6, "nt": "GCCGACTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTTGTCGTTGGTTCAATCCCAATAGTCGGCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 282633, 282635 ], "id": "AHCKFKLKGB_17", "locus": "GCA013367975_00493" }, { "type": "tRNA", "sequence": "contig_1", "start": 282680, "stop": 282755, "strand": "+", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 66.5, "nt": "GGCCCTATGGTGAAGCGGTTAACACATATGGTTTTCATCCATACATTCGTGGGTTCAAGTCCCGCTAGGGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 282713, 282715 ], "id": "AHCKFKLKGB_18", "locus": "GCA013367975_00494" }, { "type": "tRNA", "sequence": "contig_1", "start": 282758, "stop": 282833, "strand": "+", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 82.1, "nt": "GGAGAATTAGCTCAGCTGGGAGAGCATTGCCCTTACAAGGCAAGGGTCGTGGGTTCGAGCCCCTCATTCTCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 282791, 282793 ], "id": "AHCKFKLKGB_19", "locus": "GCA013367975_00495" }, { "type": "cds", "sequence": "contig_1", "start": 282961, "stop": 283965, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280AD9DE", "UniRef:UniRef90_UPI00280AD9DE" ], "nt": "TTGGTTATATTAATAGTTACTGTAACACTTGTATTAAAAGGAAATAATATTCACGTTTATGGACTTTTTAATACAAATGCAGAACATGGATTTTTAATAAGTTATTTAACACTTATATATAGTTTTTTATCAACACTTCAATCAATGCTTACTATATTAGTTTATGCTAGAATTGGTATTGCATCTGCTGTTAGGGTTAATACGCTTTTAAGTTTGCCAAACACACACATAAAAAACCACTCAAAGGGAATTGTGATAGAAAATCCTGTTGGCAATATAACATTTGATCATGTATATTTTAGATATAATGAAAATTTGCCTAAATATCAACTAACAGATGCTTCATTTAATGCAAAAGCAGGACAAACTATTGCTTTAGTTGGAGAAACTGGAGCAGGTAAAACTACTATTATAAATTTATTGAATCGTTTTTATGATTATGAAAAAGGTTCAATAAAGATAGATGGAATAGAGCTAAGAGAAATTTCAAAAGACAGTTTAAGAGATTTTACGGCAGTTGTTTTACAAGATAGCTTTATGTTTAATGAAAGTATTTTAAATAACCTTTTAGTAGGAAATCCAAAAGCAACCAAAGAAGATGTAATAAGGGCAGCAAAACTAGCATCTGCTCATCATATTATTGAAAAGTTAGAACATGGATATGATACTATAATTGATAATAGCACTTCAATATTATCTAAAGGAGAAAAGCAACTTCTTTCAATAGCTAGAGCAATATTAGGAAACAAAAAAATCTTAATATTAGATGAAGCAACATCGAACGTAGATTCTAATACAGAAAAAATAATTCAACAAGCATTAAAAGAAAATATCATGGTTGGTAAAACATCAATAGTTATTGCCCACCGTCTAAGCACGATTAAAAATGCTGATTTAATCTTAGTTGTGGATGATGGTCGCATTATTGAACAAGGAACCCATAAGCAATTGCTAGAATTAAATAAAAAATATGCAAGGTTATATTACTCACAATTTGATGATTAA", "aa": "MVILIVTVTLVLKGNNIHVYGLFNTNAEHGFLISYLTLIYSFLSTLQSMLTILVYARIGIASAVRVNTLLSLPNTHIKNHSKGIVIENPVGNITFDHVYFRYNENLPKYQLTDASFNAKAGQTIALVGETGAGKTTIINLLNRFYDYEKGSIKIDGIELREISKDSLRDFTAVVLQDSFMFNESILNNLLVGNPKATKEDVIRAAKLASAHHIIEKLEHGYDTIIDNSTSILSKGEKQLLSIARAILGNKKILILDEATSNVDSNTEKIIQQALKENIMVGKTSIVIAHRLSTIKNADLILVVDDGRIIEQGTHKQLLELNKKYARLYYSQFDD", "aa_hexdigest": "4c02778f683b5dfd98c36001c358ad2c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 282961, "stop": 284265, "observations": { "mutation": [ 283989 ], "stop": [ 283989 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280AD9DE", "query_cov": 1.0, "subject_cov": 0.6958333333333333, "identity": 0.988, "score": 625.0, "evalue": 1.52e-223, "gene_start": 1, "gene_end": 334, "reference_start": 147, "reference_end": 480, "reference_sequence": "MIIKNQVILFLFLVNDINSLSETIFKLLNELIQCAINVILSVIFLSMVSLVLSAVVLPVLFLFSFIGILITKKAQKAFIQTRKHFGELNAFVEEMLTNSKIIKTFEQQEQVNNHGKQIARNIFNTAFTGDLNNQIINPWIVFTNNILVILIVTVTLVLKGNNIHVYGLFNTNAEHGFLISYLTLIYSFLSTLQSMLTILVYARIGIASAVRVNTLLSLPSTHIKNHSKGIVIENPVGNITFDHVYFRYNENSPKYQLTDASFNAKAGQTIALVGETGAGKTTIINLLNRFYDYEKGSIKIDGIELREISKDSLRDFTAVVLQDSFMFNESILNNLLVGNPKATKEDVIRAAKLASAHHIIEKLEHGYDTIIDDSTSILSKGEKQLLSIARAILGNKKILILDEATSNVDSNTEKIIQQALKENIMVGKTSIVIAHRLSTIKNADLILVVDDGRIIEQGTHKQLLELNKKYARLYYSQFDD" }, "paralog": false, "identity": 0.9907834101382489, "score": 825.0, "evalue": 1.08e-304, "description": "Internal stop codon at 283989. Nonsense mutation around 283989.", "psc": { "uniref90_id": "UniRef90_UPI00280AD9DE", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00280AD9DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280AD9DE", "UniRef:UniRef50_UPI00280AD9DE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00280AD9DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280AD9DE" ], "product": "ABC transporter ATP-binding protein" } }, "id": "AHCKFKLKGB_515", "locus": "GCA013367975_00496" }, { "type": "cds", "sequence": "contig_1", "start": 283987, "stop": 284688, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGTTTTGTTTTCATTTTTTCATGCAATTTTTTATGCAATAGGTTCATTTTTAATTGGATATATTTTTACAATATGTTTAACAAAAGATGTATTAGATCATAAAACACCTTTTAATTTAAAATTATTTATAGGTATGGCTTCATGTTTGGTTATATCGTTTATATTGTATGGAATTTTTAGATATGTTTCAGGAGTATTCTTTGTAAAAATAACTTTTTCAGTTTGCGAGAATATAAGAAAAACTGCCATGAAAAATCTTTTATATATTCCTGTTTCGTATTTTGATAATCAAAAATCAGGTAATATTATTTCTATTTTAGTAAATGATATTAATAGTTTATCTGAAACTATATTTAAATTATTAAACGAATTAATCCAATGTGCTATAAATGTTATTTTATCTGTAATATTTTTATCAATGGTTAGTTTAGTATTATCTGCTGTTGTTTTACCTGTTCTATTCTTATTTTCATTTATAGGAATATTAATTACTAAAAAAGCTCAAAAAGCATTTATTCAAACCAGAAAACATTTTGGGGAATTAAATGCCTTTGTTGAAGAAATGCTAACTAATTCTAAAATTATTAAAACTTTTGAACAACAAGAACAAGTTAATAATCATGGTAAACAAATAGCAAGAAACATATTTAACACAGCCTTCACAGGAGATCTTAATAACCAAATAATTAATCCTTGA", "aa": "MAVLFSFFHAIFYAIGSFLIGYIFTICLTKDVLDHKTPFNLKLFIGMASCLVISFILYGIFRYVSGVFFVKITFSVCENIRKTAMKNLLYIPVSYFDNQKSGNIISILVNDINSLSETIFKLLNELIQCAINVILSVIFLSMVSLVLSAVVLPVLFLFSFIGILITKKAQKAFIQTRKHFGELNAFVEEMLTNSKIIKTFEQQEQVNNHGKQIARNIFNTAFTGDLNNQIINP", "aa_hexdigest": "511f8b17b57075d707311b7b34376e9d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26396.00490000002, "isoelectric_point": 9.158330345153807 }, "id": "AHCKFKLKGB_516", "locus": "GCA013367975_00497" }, { "type": "cds", "sequence": "contig_1", "start": 284689, "stop": 284784, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGAACTAAAGAAAAGCAAAAACTTTCATTTATTACTTTGTTTAAATTAGTTTTTAAATATTCAGGTGAATCAAAAACAAAATTTTGA", "aa": "MKKRTKEKQKLSFITLFKLVFKYSGESKTKF", "aa_hexdigest": "9e033a066f30cb864e5c512b3f8fafc4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3770.5296000000008, "isoelectric_point": 10.363437843322753 }, "id": "AHCKFKLKGB_517", "locus": "GCA013367975_00498" }, { "type": "cds", "sequence": "contig_1", "start": 284799, "stop": 285767, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFCB5D" ], "nt": "GTGGTGCTTTATTCATTAGTTGTTCATGATGTTATTTCTAATGTAGCAACATATGATACAATTGTAAGATTTAATATTTTTATTGACTATTCATTTTATGTAACGTTTGGTGTTATATTGGGGGCAGTATTTATTTCAGCATTTGTAAAAGGAAAAATTTCTGCTACTAGAGTTATAGAAGTATTAAATACTAACGAAGATAAAATTTATGTATCAGATGGTAAAAAAATAACAAAAAACTTCGATTTAAAAGTTGAAAATTTAAGCTTCAAATATTATTCTTCAAATCCAAATTATACTTTACACAATATAAGTTTTAGCCTACCGTTTGGAAAAACTCTTGGATTAGTTGGCCCTACTGGAAGTGGCAAGAGCATACTTGCAAATTTATTGGTTAATAACTATCTATATAATGAAGGATCGATTTTAATTGGGGATAATGAAATTAAAGAAGTTAATACAAAAAATTTACATGAAACGATCGGAATTGTTTACCAAGATGCCTTATTAAATACAGGAACAATAAAAGAAAATATGCTTTTTGCTAAAAATGATGCAACAGATGAAGAAATAAATCAAGCATTACGTGACGCTTGTGCATATGATTTTGTTTATAAATTTGAAGAAAATTTAGATCATGTCATTACTCAAGGGGCAACAAACCTTTCAGGTGGCCAAAAACAACGTCTTTCAATTGCAAGAACTTTATTAACTAAACCAAAAATTTTAATATTAGATAACTCAACTAGTGCTTTAGATAATATTACTGCACACCAAGTTTTACAAAATATAAAAAATAAATATTCATGTTCAACTATAATAATTAGCCAAAAATTGCCATTAATAAAAAATGCTGATGAAATTATTGTTTTATCTGATGGAAATATGTTGGATAAAGGAAATCATGAAGAATTAATGAAATCATGTTCATATTATAATGAATCATATAATAACCAATTAAGTCAATAA", "aa": "MVLYSLVVHDVISNVATYDTIVRFNIFIDYSFYVTFGVILGAVFISAFVKGKISATRVIEVLNTNEDKIYVSDGKKITKNFDLKVENLSFKYYSSNPNYTLHNISFSLPFGKTLGLVGPTGSGKSILANLLVNNYLYNEGSILIGDNEIKEVNTKNLHETIGIVYQDALLNTGTIKENMLFAKNDATDEEINQALRDACAYDFVYKFEENLDHVITQGATNLSGGQKQRLSIARTLLTKPKILILDNSTSALDNITAHQVLQNIKNKYSCSTIIISQKLPLIKNADEIIVLSDGNMLDKGNHEELMKSCSYYNESYNNQLSQ", "aa_hexdigest": "1e3134162cc4f909a70cca444715aa55", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DFCB5D", "query_cov": 0.9440993788819876, "subject_cov": 0.9589905362776026, "identity": 0.52, "score": 315.0, "evalue": 1.35e-103, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021DFCB5D" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFCB5D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFCB5D" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "AHCKFKLKGB_518", "locus": "GCA013367975_00499" }, { "type": "cds", "sequence": "contig_1", "start": 285846, "stop": 286103, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATTGGTGGATGTATTTTAGCCGTCATGAATAACGTAAAAATGGCAATAGGATTAATTTTGTTAGTTCCGATAATTTTAACAATAGTTTCTATTATGGCCAAAAAAGCAAGTCCTTCAATTAAAAAAGCTCAATTAGCCGTTGAAGAAATAACTAAGCAAGTTGATGAAAATATATTGGGATCTCGTTTGATAAAAATATATGATTTATATAAAAATAGAGAAGAAAAGTTTGCAAAAATAAATAAAACATGA", "aa": "MIIGGCILAVMNNVKMAIGLILLVPIILTIVSIMAKKASPSIKKAQLAVEEITKQVDENILGSRLIKIYDLYKNREEKFAKINKT", "aa_hexdigest": "f4b0a80b858dff6d1eaa5909d85d28fe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9426.415499999992, "isoelectric_point": 9.673819541931152 }, "id": "AHCKFKLKGB_519", "locus": "GCA013367975_00500" }, { "type": "cds", "sequence": "contig_1", "start": 286149, "stop": 286541, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGTAGGAAGCTTTATTTCTTTAATTTCAGTTATATTTGAGTTGATTACTCCTACATTGATTTCTCAATTCATTAGATTGCTTTTTATTGAAAATAAAAATAGCCAAGTAGATTTAAGTTTTTTTAATGGATTAATAAACTTTACATTTAATAATGCTATTAATGCTCGCAATTATATGATTTATTCTATAATAGTTTTAACTATAATTTCAATGTCTTTAACATTTGGCTGCACTAGTTTAATTGTATATGCAGCAGAAAGAAGTAGTAGATATATTAGAGAAAACTTTATTAGAAAATTAAATACATTAAGTTTGAAAAATATATCTGATTTGAAACCCGAATCATTAATTACTAGGGTGAGTGATGATATTGCGGTTTTCTGA", "aa": "MFIVGSFISLISVIFELITPTLISQFIRLLFIENKNSQVDLSFFNGLINFTFNNAINARNYMIYSIIVLTIISMSLTFGCTSLIVYAAERSSRYIRENFIRKLNTLSLKNISDLKPESLITRVSDDIAVF", "aa_hexdigest": "62971badabec682713fe13ec023314ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14847.25269999998, "isoelectric_point": 8.88685398101807 }, "id": "AHCKFKLKGB_520", "locus": "GCA013367975_00501" }, { "type": "cds", "sequence": "contig_1", "start": 286770, "stop": 287585, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATCTTACTGTTTCATTTAAAGATTATGAAAATAGTATTAAGAAATCAAAATTTGTTGAAATTGATTTAAGTAAATTGGGCGTTGATGAATATAACAAGATTAGAAAACAAAATAATGAAACTGAAATAGATGACCAACAAGCCCCAACATCAACTATTGAAGACCAAGATGTAAAAGAATTAAATAAAGATGCATTCTTGAAATATTTCAAAGAAAACAAAGATAAATTATCTAATGATGTAGTTAAAAATAATTCAACATTTATGTTTGTAGAAAAATTGAAAAATATTAGTCTTTTAAGCAAAGACATTGAAAAAGAAATGATTGAAAAAGGTGATAATTACATTTCGGTTCAAAATTTTGATTATGATAAAACAACATTAAAATTAAAACATTCAATGTTTGCGTTTGAATATACAAAACATATGGTTAATTTACAAAATGTTTATATCTTTTCATTTCCTAAAATAGAAAATAATAAAGTAAAATCAATTAGAGTTTCTATAGTTTCTATTCAAGATTTTCAAAATAATGATTTTAGCAATATGTCATCTAAACGTGTCAATGTTAATGAAAAGATAAATGAAGATAAAGACTTTATGCAAAAAATAGTTTTAAAAGAACATTTTAGAGAATTAGTAACCGTAAAATACATTGGCGATAAAAAAACAGTTGATGAAGTAATTAAAGATGATATTTCTAAATTCGAATGCAAATTACCAGAAGGATTAACATTTGAAAAAATTTCATTCAAAAAAGCAACCAAAAATAAGAAGGAATATATAAAATATACAATCACTGCTAAATGA", "aa": "MINLTVSFKDYENSIKKSKFVEIDLSKLGVDEYNKIRKQNNETEIDDQQAPTSTIEDQDVKELNKDAFLKYFKENKDKLSNDVVKNNSTFMFVEKLKNISLLSKDIEKEMIEKGDNYISVQNFDYDKTTLKLKHSMFAFEYTKHMVNLQNVYIFSFPKIENNKVKSIRVSIVSIQDFQNNDFSNMSSKRVNVNEKINEDKDFMQKIVLKEHFRELVTVKYIGDKKTVDEVIKDDISKFECKLPEGLTFEKISFKKATKNKKEYIKYTITAK", "aa_hexdigest": "dd34b0382409010bf6e873380f779107", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31945.18210000002, "isoelectric_point": 8.710339546203613 }, "id": "AHCKFKLKGB_521", "locus": "GCA013367975_00502" }, { "type": "cds", "sequence": "contig_1", "start": 287652, "stop": 288569, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J821", "UniRef:UniRef90_D1J821" ], "nt": "TTGGTAAATCCTGAAAAACCTCTTAGAAGTGGAGACAAGTTAAACTCTAAATTGTTATTAACAAAAGATGAAAAAGGAAACTTTATTTTGAAATATAAAGTTGCAATTTTTAAACAAAATGGTAAACATATTATTTCAAACAAGGTTTATGAAAGTAATTTGGGAAACTTAGATTCTGGATTAGATCAAGTTTCAGAAGAAGATTTTAAAGAAGTTTCAAAAAATCTAGTCTTTGACTATCCTAACAAACAAGAAACATACATAAAAGACGCGGACACATCAAAAGTATTTTTAAAAACTATTAAAGAAAACTTTTCAATTGTTAAAGATTCATTAAAGATTACTAAAAATACAGGAGAAGATGGAACATATGACTTGTCAATTCTTTACTATTTATCATATAAAAATAAACAAATCTCAAGTCAAAAATATATTTATGAAATCAAAGGTTTTAAATTAACTCCAGAATTGCAAGAAAAAATAGATAAGGCAAGAAAAGAACTAAAAGATCAAATTGAAAAAACAAATGTTTTAGTTGCAAATGCCAAGTCATATCAAGATATTTTAAAAAATAAACAAATAAATAATTTTGATAAAAAACCAAATTTTGCAGTTCAAGGCTATGATCAAGCTTCATATTCAGTAGTATTAAGCAATTTAAAAGTTGAAGATAAAAAAATAACATTGACTTTAACTTTGTCAAGTCTTTTAAATAAAGAAATAAGTGAATCTAAAACTATTGAAGTTAAATTAGATAAATTTGCAAATGGTTTAGATAATATTGCATTGCTATCACCAGAAAAACAAGCTGAACTTTTAAACAAAGCTTTAGAAACTTCAATTTATCCATTTTATAGTAAAGACAAATATTACATTTCAAAGAAATCTATTGACAAATTAGAAAATAAATCATACTGA", "aa": "MVNPEKPLRSGDKLNSKLLLTKDEKGNFILKYKVAIFKQNGKHIISNKVYESNLGNLDSGLDQVSEEDFKEVSKNLVFDYPNKQETYIKDADTSKVFLKTIKENFSIVKDSLKITKNTGEDGTYDLSILYYLSYKNKQISSQKYIYEIKGFKLTPELQEKIDKARKELKDQIEKTNVLVANAKSYQDILKNKQINNFDKKPNFAVQGYDQASYSVVLSNLKVEDKKITLTLTLSSLLNKEISESKTIEVKLDKFANGLDNIALLSPEKQAELLNKALETSIYPFYSKDKYYISKKSIDKLENKSY", "aa_hexdigest": "bda397c0fcc2d56f3cbf73e1d4582019", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 287349, "stop": 288869, "observations": { "mutation": [ 287654, 288599, 288743 ], "stop": [ 287654, 288599, 288743 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J821", "query_cov": 1.0, "subject_cov": 0.5222602739726028, "identity": 0.987, "score": 563.0, "evalue": 7.74e-198, "gene_start": 1, "gene_end": 305, "reference_start": 142, "reference_end": 446, "reference_sequence": "MKKHTKILLALGSVATSVFAIPLVAAGCIKTKKPEVKPEEPKKPEGEKPGTTPEVKPEDPSHEEETNKNDADKYGEESISKITWNAENLEFIKKVLNNLDKYKILFERNNKNIMAFEGAYDFKNQEFAIAKWNSKSGVSVQLVNPEKPLRSGDKLNSKLLLTKDEKGNFILKYKVAIFKQNGKHIISNKVYESNLGNLDSGLDQVSEEDFKEVSKNLVFDYPNKQETYIKDADTSKVFLKTIKENFSIVKDSLKITKNTGEDGTYDLSILYYLSYKNKQISSQKYIYEIKGFKLTPELQEKIDKARKELKDQIEKTNVLVANAKSYQDILKNKQINNFDKKPNFAVQGYDQASYSVALSNLKVEDKKITLTLTLSSLLNKEISESKTIEVKLDKFANGLDNIALLSFEKQDELLNKALETSIYPFYSKDKYYISKKSIDKLENKSYWINNKNSEINYTLGELKFEGDKCLINLTVSFKDYENSTKKSKFVEIDLSKLGVDEYNKIRKQNNETEIDDQQAPTSTIEDQDVKELNKDAFLKYFKENKDKLSNDEVKNISTFMFVEKLKNISLLSKDIEKRNDWKRW" }, "paralog": false, "identity": 0.9624505928853755, "score": 945.0, "evalue": 0.0, "description": "Internal stop codon at 287654, 288599, 288743. Nonsense mutation around 287654, 288599, 288743.", "psc": { "uniref90_id": "UniRef90_D1J821", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J821", "UniRef:UniRef50_D1J821" ] }, "pscc": { "uniref50_id": "UniRef50_D1J821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J821" ], "product": "Variable surface lipoprotein" } }, "id": "AHCKFKLKGB_522", "locus": "GCA013367975_00503" }, { "type": "cds", "sequence": "contig_1", "start": 288741, "stop": 288992, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATACAAAAATTTTATTAGCCTTAGGTAGTGTTGCAACCTCGGTTTTTGCAATACCATTAGTGGCAGCAGGTTGCGTAAAAACAAAAAAACCTGAAGTTAAACCAGAAGAACCTAAAAAACCAGAAGGCGAAAACCCAGGAACAACACCTGAAGTTAAACCAGAAGATCCAAGTCATGAAGAAGAAACAAATAAAAATGATGCTGATAAATATGGCGAAGAATCAATTTCAAAAATAACATGA", "aa": "MKKHTKILLALGSVATSVFAIPLVAAGCVKTKKPEVKPEEPKKPEGENPGTTPEVKPEDPSHEEETNKNDADKYGEESISKIT", "aa_hexdigest": "870baa272249d35f31f855643b8996b3", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8969.078999999994, "isoelectric_point": 5.690515327453612 }, "id": "AHCKFKLKGB_523", "locus": "GCA013367975_00504" }, { "type": "tRNA", "sequence": "contig_1", "start": 289270, "stop": 289345, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 81.9, "nt": "GCATCCTTAGCTCAGTTGGCAGAGCAAGGGACTCTTAATCCCTGGGTCGTGGGTTCGAGCCCCACAGGATGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 289338, 289340 ], "id": "AHCKFKLKGB_30", "locus": "GCA013367975_00505" }, { "type": "tRNA", "sequence": "contig_1", "start": 289447, "stop": 289531, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 60.8, "nt": "GCGCGAGTGGTGAAATTGGCAGACACGTTAGTTTTAGGCACTAATGCTTTGCGGCGTGAGGGTTCAAGTCCCTCCTCGCGTACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 289501, 289503 ], "id": "AHCKFKLKGB_29", "locus": "GCA013367975_00506" }, { "type": "tRNA", "sequence": "contig_1", "start": 289535, "stop": 289610, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 88.2, "nt": "GCCGTTTTAGCTCAGTTGGCAGAGCAACTGACTTGTAATCAGTAGGTCGTAGGTTCAAGTCCTATAAACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 289575, 289577 ], "id": "AHCKFKLKGB_28", "locus": "GCA013367975_00507" }, { "type": "cds", "sequence": "contig_1", "start": 289819, "stop": 290607, "strand": "+", "frame": 1, "gene": "truB", "product": "tRNA pseudouridine(55) synthase TruB", "db_xrefs": [ "COG:COG0130", "COG:J", "EC:5.4.99.25", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "KEGG:K03177", "SO:0001217", "UniRef:UniRef50_A0A501X9B0", "UniRef:UniRef90_D1J822" ], "nt": "ATGTTTTATAAGTTATATAAAAAAAGAGGCCAATCAAGTTTTTTTGCAATAAAAAAATTTGCAAAAAATAATAATATCAATAAAGTTGGACATTGTGGAACATTAGATCCCGAGGCTGAAGGTCTTTTAATCGTAGCAACAGATGAAGATACAAAGGCCTTGTCTTTAATTGAAAATCAAAACAAGGAATACTATGTGGAAGCGAAATTCCATTACTCTTCAGATAGTTTTGATTTAGGAACATATGTAACTAAAATACAAAATTTTCAAATAATAACTAAATCTCAAATTATACAAGCACTAGAAATATTAAAAAAACAAACAAAACAAGTACCTCCAATTTATAGTGCAAAAAAAATTAATGGAACAAGAGCATACAAACTTGCTAGAGATAATAAAGAATTTAGTTTAAAAGCCAATGATATAAAAATAAATAATTTAGAATTAATTGAATTTAATGAAGTGAATCAATCAATCATCATTAAGGTCAATGTTTCAAAAGGCACATATATTAGATCATTGATTCATGATTTAGCTAATATATGTAACACAGATGCAGTAGTAGTTGTTTTAAAGCGAATTAAAATTGGCAATATATGTTTAAATGGTCAAGAAGAATTTGAAAAAATTACAAACTTAAACGAACTTTTTAATGTAAAATTATTTAAGCTAACCAAAAATGATATTCAAATATTAGCAAACCAAAATTCAAATAGTTGCTACTTTGAATCATTGAAAAATTTTTGCGGAAACATTATTTTTATTTACTTGAATAACATAGTAGGATGA", "aa": "MFYKLYKKRGQSSFFAIKKFAKNNNINKVGHCGTLDPEAEGLLIVATDEDTKALSLIENQNKEYYVEAKFHYSSDSFDLGTYVTKIQNFQIITKSQIIQALEILKKQTKQVPPIYSAKKINGTRAYKLARDNKEFSLKANDIKINNLELIEFNEVNQSIIIKVNVSKGTYIRSLIHDLANICNTDAVVVVLKRIKIGNICLNGQEEFEKITNLNELFNVKLFKLTKNDIQILANQNSNSCYFESLKNFCGNIIFIYLNNIVG", "aa_hexdigest": "bd0e56cb55050564543079d0a4248b1a", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J822", "query_cov": 1.0, "subject_cov": 0.9128919860627178, "identity": 0.9890000000000001, "score": 502.0, "evalue": 4.08e-179, "valid": true, "gene": "truB", "product": "tRNA pseudouridine(55) synthase TruB", "ec_ids": [ "5.4.99.25" ], "uniref50_id": "UniRef50_A0A501X9B0", "cog_id": "COG0130", "cog_category": "J", "kegg_orthology_id": "K03177", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501X9B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X9B0" ], "product": "tRNA pseudouridine synthase B" }, "genes": [ "truB" ], "id": "AHCKFKLKGB_524", "locus": "GCA013367975_00508" }, { "type": "cds", "sequence": "contig_1", "start": 290691, "stop": 291062, "strand": "+", "frame": 3, "gene": "cof", "product": "YcsE-related riboflavin metabolism phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_D1J823", "UniRef:UniRef90_D1J823" ], "nt": "ATGACAAAATACAAAATGATAGTATTTGATATTGATGGAACATTGTTGCCGTTTGGAGTTACTCAATTAACACCTAGAATGCAAGAAATGTTTGAAAAATTAAAAGAACAAGGCTATGTAAATGTGTTGTGTTCTGGTCGTGATATTTTTACAGTAGGCGCCCAAATAGATAACCCTTATGTTGATTATTTTATAGGTGCAAATGGAACTTTTATTCTTGATTTAAGAACAAAAGAATATATTTTTGAAAAAACTATTAATTACAAAGATTTTGTTGAATTTAGAAAATATGCAAGCGAACATAAATTATCTTTTTCTTTTGTTGGAAACAAATGGGGATATTACAATGAATTATTTAATATAGATCATTGA", "aa": "MTKYKMIVFDIDGTLLPFGVTQLTPRMQEMFEKLKEQGYVNVLCSGRDIFTVGAQIDNPYVDYFIGANGTFILDLRTKEYIFEKTINYKDFVEFRKYASEHKLSFSFVGNKWGYYNELFNIDH", "aa_hexdigest": "1d96cd0a73be2f35c123cce0dec97131", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 290691, "stop": 291365, "observations": { "mutation": [ 291060, 291213, 291228 ], "stop": [ 291060, 291213, 291228 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J823", "query_cov": 1.0, "subject_cov": 0.4606741573033708, "identity": 0.9840000000000001, "score": 256.0, "evalue": 9.87e-85, "gene_start": 1, "gene_end": 123, "reference_start": 1, "reference_end": 123, "reference_sequence": "MTKYKMIVFDIDGTLLPFGVTQLTPRMQEMFEKLKEQGYVNVLCSGRDIFTVGAQIDNPYVDYFVGANGTFILDLRTKEYIFEKTINYKDFIEFRKYASEHKLSFSFVGNKWGYYNELFNIDHWFYRPYKDKFIPEKEFESQHDKNYLITISTPTPSIVAADLEKFFKEKNLDLWILAQWNGGIFISAKGITKAVGLKILGDLLNISLDEMVAFGDSENDIEMLEKVGLGIAMGNGEDVTKDAAKEICLPVTEDGPYWKLRDKGFFD" }, "paralog": false, "identity": 0.9776785714285714, "score": 443.0, "evalue": 8.2e-161, "description": "Internal stop codon at 291060, 291213, 291228. Nonsense mutation around 291060, 291213, 291228.", "psc": { "uniref90_id": "UniRef90_D1J823", "gene": "Cof", "product": "YcsE-related riboflavin metabolism phosphatase", "uniref50_id": "UniRef50_D1J823", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J823", "UniRef:UniRef50_D1J823", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_D1J823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J823" ], "product": "Cof-type HAD-IIB family hydrolase" } }, "id": "AHCKFKLKGB_525", "locus": "GCA013367975_00509" }, { "type": "cds", "sequence": "contig_1", "start": 291321, "stop": 291464, "strand": "+", "frame": 3, "gene": null, "product": "Hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W0C783" ], "nt": "ATGGTGGCATTTGGTGATTCAGAAAATGATATTGAAATGCTTGAAAAAGTAGGATTAGGTATTGCTATGGGCAATGGAGAAGATGTTACTAAAGATGCTGCAAATGAAATTTGCTTGCCAGTTACTGAAGATGGTCCATATTGA", "aa": "MVAFGDSENDIEMLEKVGLGIAMGNGEDVTKDAANEICLPVTEDGPY", "aa_hexdigest": "574b0db1bae9dc23f0128d424f25fbcc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W0C783", "query_cov": 0.9361702127659575, "subject_cov": 0.8, "identity": 0.568, "score": 53.5, "evalue": 1.64e-08, "valid": false, "product": "Hydrolase", "uniref50_id": "UniRef50_A0A2W0C783" }, "pscc": { "uniref50_id": "UniRef50_A0A2W0C783", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W0C783" ], "product": "Hydrolase" }, "genes": [], "id": "AHCKFKLKGB_526", "locus": "GCA013367975_00510" }, { "type": "cds", "sequence": "contig_1", "start": 291509, "stop": 292288, "strand": "+", "frame": 2, "gene": "spoU", "product": "tRNA/rRNA methyltransferase", "db_xrefs": [ "COG:COG0566", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A3B0PPX9", "UniRef:UniRef90_D1J824" ], "nt": "ATGGTTGATTTTTATTTATTTTATTTTAAAATACTTATATTAGGAAAAAAACACATGGCAATTACAAGTACACAAGATCCAAAAATTCTAAAATATAAAAAATTACTTACTCCCAAAGGCAGACAAGAACAACAATTGTTTATTGTTGAAGGTTATCATTTAGTAAAAGAAGCAATCAAGGCAAATATAGTTATTGACATTTTAGAATTCAATGACATACTAACTTATAAAAACTCAACTCAAGTAACTAGAGCAATTATTAAAACATTATCAACAACGCAAACACCTCAAAATATCATTGCAATTTGTAAAATACAAAATTTAAATATTAAAGCAAATAAAATAATAGCCCTTAATAAATTACAAGATCCCGGAAATATTGGTACTATTTTTCGACTTGCTAAATCTTTTGGCTTTGATAGTGTTATAGTTGAAAATATAGATATATACAATGAAAAGATAATTCGTTCATCACAAGGAGCAATGTTTTCACTAAACATTATTAAGACAAATGATTTAAGCAAAGAACTGTCAGCCTTTAAAAAAGATGATTTTTTAGTTTATAGTACAACATTAAATAAAAATTCAATTAAGCTCAATGACATTGATTTTAGAAATAAAAAAATTATTGTTGTTTTGGGAAATGAAGGAAATGGTATAGACAAAAAAATTATTGACTTAAGTGATTTTAATGTTTATATTCCTATTGAATTTGAAAGTCTCAATGTTGCTTGCTGCGGCGCAATTATTATGAATAAAATTTATAATGGAGATAAATAA", "aa": "MVDFYLFYFKILILGKKHMAITSTQDPKILKYKKLLTPKGRQEQQLFIVEGYHLVKEAIKANIVIDILEFNDILTYKNSTQVTRAIIKTLSTTQTPQNIIAICKIQNLNIKANKIIALNKLQDPGNIGTIFRLAKSFGFDSVIVENIDIYNEKIIRSSQGAMFSLNIIKTNDLSKELSAFKKDDFLVYSTTLNKNSIKLNDIDFRNKKIIVVLGNEGNGIDKKIIDLSDFNVYIPIEFESLNVACCGAIIMNKIYNGDK", "aa_hexdigest": "3c5f9be6c585dc5a60ede321074ace09", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J824", "query_cov": 0.9305019305019305, "subject_cov": 1.0, "identity": 0.975, "score": 457.0, "evalue": 4.59e-162, "valid": true, "gene": "SpoU", "product": "tRNA/rRNA methyltransferase", "uniref50_id": "UniRef50_A0A3B0PPX9", "cog_id": "COG0566", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PPX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PPX9" ], "product": "tRNA/rRNA methyltransferase" }, "genes": [ "spoU" ], "id": "AHCKFKLKGB_527", "locus": "GCA013367975_00511" }, { "type": "cds", "sequence": "contig_1", "start": 292447, "stop": 292920, "strand": "+", "frame": 1, "gene": "ribF", "product": "FAD synthase", "db_xrefs": [ "COG:COG0196", "COG:H", "EC:2.7.1.26", "EC:2.7.7.2", "KEGG:K11753", "SO:0001217", "UniRef:UniRef50_A0A448ZYJ6", "UniRef:UniRef90_D1J825" ], "nt": "ATGATGTTTAAAAATCCAGTTAAAAATTCTCAGGTATTAACAAAAAAAGCAATGCAACCAAAAACCAGATTATATACATTAGCAATGTTGGGATTTGATTATGTTTTTTTAGTTGATGTAAACCAAACGATATTGAATTGTAGTTATAGCGATTTTATTCAAGGATTAAAGATAAATAATGTTAAAAATGTTGTTTGTGGTCAAGATTTTAGGTTTGGGTTCAATAGAGAGGGTAACATTGATAAATTAAAAAAATATTTCAATGTATATGTTGCCAATGAATTAAAGGTAAATAAACAAAAAATTTCTTCAACAATAATCAATGAATTTATTGAAGAAGGAAACATAAATGGCTTGAACAAATTACTAATAGACAAATATTCAATAATTGTAAATTCTCAAAAATTTAATTTCAAATATCCAGAACATTTAAATCAATTAAAAGCAGGAATTTATATTGCCAATGTCGTTTGA", "aa": "MMFKNPVKNSQVLTKKAMQPKTRLYTLAMLGFDYVFLVDVNQTILNCSYSDFIQGLKINNVKNVVCGQDFRFGFNREGNIDKLKKYFNVYVANELKVNKQKISSTIINEFIEEGNINGLNKLLIDKYSIIVNSQKFNFKYPEHLNQLKAGIYIANVV", "aa_hexdigest": "da5ea319c3bce68c2d1989e8223fe2e0", "start_type": "ATG", "rbs_motif": null, "genes": [ "ribF" ], "truncated": "both", "pseudogene": { "start": 292288, "stop": 293160, "observations": { "mutation": [ 292303, 292918 ], "stop": [ 292303, 292918 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J825", "query_cov": 1.0, "subject_cov": 0.5358361774744027, "identity": 0.9940000000000001, "score": 307.0, "evalue": 1.14e-103, "gene_start": 1, "gene_end": 157, "reference_start": 54, "reference_end": 210, "reference_sequence": "MQVFDWDFKAKLNINEPLTICLGSFETLHLGHNELFKIAKDLKQSYPDQKLAIMMFKNPVKNSQVLTKKAMQPKTRLYTLAMLGFDYVFLVDVNQTILNCSYSDFIQGLKINNVKNVVCGQDFRFGFNREGNIDKLKKYFNVYVANELKVNKQKISSTIINEFIEEGNINGLNKLLIGKYSIIVNSQKFNFKYPEHLNQLKAGIYIANVVWNNIEYHGLIFINKNQFDSNNNLQENNIIYLFDIDYFISKYQELYIEFLQCIRHINNNSENQISTNDIEIGKKYFISKEKMNS" }, "paralog": false, "identity": 0.9689655172413794, "score": 565.0, "evalue": 6.03e-208, "description": "Internal stop codon at 292303, 292918. Nonsense mutation around 292303, 292918.", "psc": { "uniref90_id": "UniRef90_D1J825", "gene": "RibF", "product": "FAD synthase", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_A0A448ZYJ6", "cog_id": "COG0196", "cog_category": "H", "kegg_orthology_id": "K11753", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J825", "EC:2.7.1.26", "EC:2.7.7.2", "UniRef:UniRef50_A0A448ZYJ6", "COG:COG0196", "COG:H", "KEGG:K11753" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ6" ], "product": "FAD synthase" } }, "id": "AHCKFKLKGB_528", "locus": "GCA013367975_00512" }, { "type": "cds", "sequence": "contig_1", "start": 293224, "stop": 293736, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATGGCTATGAAATTGTTAAACAAATGTTTAATTATGGCCATACTTATAGAGAAAAAGAAATCAATAAAATGATAAAAGATATATTCGTGAACCTAGCTTTAAATAAAGACGAATATACTTATAAATTTCTTTGTGAAAATTCTATAAATTATTTTGTTAATAATAAAATGTTAGAAAAATTAATAAATCCTGATGGCGAAATCTTTTATCATGATGTTTCAAAGCCAAATACTTTTAGAATGCCAAAGGATTGTGACAATAGAAAAATATCAGAAATAAGTTCGTTGGAAATGTTTGATTTAATAAAACAAGTTTTAAACAATTCAAAAAGTTCTATTTTAAAAAACAATTTAACTAATACTATTTTAAAAACATTACTAATAGCAAGAACAGAAAATAATATTTCAAAATTAAATGATCTATATGGATATTTAAATAATTTAGGGTTAATTAAAGTTATTTCAAACGGCGATATTGAATATATTGACTTGAACAACAAAGATTAA", "aa": "MLNGYEIVKQMFNYGHTYREKEINKMIKDIFVNLALNKDEYTYKFLCENSINYFVNNKMLEKLINPDGEIFYHDVSKPNTFRMPKDCDNRKISEISSLEMFDLIKQVLNNSKSSILKNNLTNTILKTLLIARTENNISKLNDLYGYLNNLGLIKVISNGDIEYIDLNNKD", "aa_hexdigest": "f432fd4670b3426146f4016bfbd517f8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19923.67160000002, "isoelectric_point": 6.740047264099122 }, "id": "AHCKFKLKGB_529", "locus": "GCA013367975_00513" }, { "type": "cds", "sequence": "contig_1", "start": 293989, "stop": 294861, "strand": "-", "frame": 1, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FAB5C6", "UniRef:UniRef90_UPI001F43A718" ], "nt": "TTGCCTTCAAATAAGTTCGTTTATGATAATAAATTAATTACTTTTCCAACATATCAAAAAACCGCCAAGAAAGACGGGGAATTGCTTCCTTTTGAAGGCGTTTCATTTATTAAGGTTAATGGAATTTATGAAGATCAAACAAACGAAATTGAAGCAAATAGAATCATTGAATTAATAAAGCAATTAAAAGATTATTATGGCAATACAAAAACATTTGGAGTTGTAACGTTTAATTTAAAACAAGCTAACTTAATTGATCACAAATTATTCTTATTTATGAATGAAAATCCTGAATATATGGATTTAGACACAGAAAATAAAATTTTTGTTAAGAACATTGAAAGTGTTCAAGGTGATGAAAGAGACATCATTATTTTAGGAATTACAAATAGTTGGGATAAAAACGGAAGATTGTCAAGATTCTTTGGACCATTAAATAATGTTGGCGGTGAAAAGAGACTAAACGTCGCAATAAGTAGAGCCAAGGAAGCAACTATTGTTGTATCTTCAATATCATATTCTGATTTAAAAGTAGACGATTTAAAAAACGAAGGACTAAGATTTTTAAAGCAATATTTACAATTTGCAGAATTTGGTTACGACAGCAAATCAGATGATATATTACAAGATGACACTAAAATATTTGATAGTGAGTTTGAAATCCAAGTTTATGATGAACTTAAGAAGCTTGGATTTAAAGTAAATACTCAAGTGGGATGTTCAAAATTTAAAATAGATTTAGCAATTGTTGATGATAAAAATCCATCGAAATATATTTTAGGTATTGAATGCGATGGCGCCACATATCATTCATCAAAAAGCGCTCGTGATAGAGATGCGATTAGACAAATGCTACTTGAGTCAAGAGGTTGA", "aa": "MPSNKFVYDNKLITFPTYQKTAKKDGELLPFEGVSFIKVNGIYEDQTNEIEANRIIELIKQLKDYYGNTKTFGVVTFNLKQANLIDHKLFLFMNENPEYMDLDTENKIFVKNIESVQGDERDIIILGITNSWDKNGRLSRFFGPLNNVGGEKRLNVAISRAKEATIVVSSISYSDLKVDDLKNEGLRFLKQYLQFAEFGYDSKSDDILQDDTKIFDSEFEIQVYDELKKLGFKVNTQVGCSKFKIDLAIVDDKNPSKYILGIECDGATYHSSKSARDRDAIRQMLLESRG", "aa_hexdigest": "d26fd8f149c5d8830ed723e546136fb2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 293686, "stop": 294834, "observations": { "mutation": [ 293805, 293961, 293973, 293991 ], "stop": [ 293805, 293961, 293973, 293991 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F43A718", "query_cov": 0.9689655172413794, "subject_cov": 0.5072202166064982, "identity": 0.993, "score": 554.0, "evalue": 6.71e-195, "gene_start": 10, "gene_end": 290, "reference_start": 19, "reference_end": 299, "reference_sequence": "MIEKSLYDFATPDFQTLPNKLITFPTYQKTAKKDGQLLPFEGVSFIKVNGIYEDQTNEIEANRIIELIKQLKDYYGNTKTFGVVTFNLKQANLIDHKLFLFMNENPEYMDLDTENKIFVKNIESVQGDERDIIILGITNSWDKNGRLSRFFGPLNNVGGEKRLNVAISRAKEATIVVSSISYSDLKVDDLKNEGLRFLKQYLQFAEFGYDSKSDDILQDDTKTFDSEFEIQVYDELKKLGFKVNTQVGCSKFKIDLAIVDDKNPSKYILGIECDGATYHSSKSARDRDAIRQMLLESRGWIIHRIWSLNWFKNKRRELEKIVDKVNQIKQKKFVVKQNEYKTIENSQFEVIEKRDNFNFEKWLPFNYSYRKTLNFIEKSNKRNPLLNGYEIVKQMFNYGHTYREKEINKMIKDIFMNLPLNKNEYTYKFLCENSINYFVNNKMLEKLINPDGEIFYHDVSKPNTFRMPKDCDNRKISEISSLEMFDLIKQVLNNSKSSILKNNLTNTILKTLLIARTENNISKLNDLYGYLNNLGLIKVISNGDIEYIDLNNKD" }, "paralog": false, "identity": 0.9764397905759162, "score": 741.0, "evalue": 3.15e-271, "description": "Internal stop codon at 293805, 293961, 293973, 293991. Nonsense mutation around 293805, 293961, 293973, 293991.", "psc": { "uniref90_id": "UniRef90_UPI001F43A718", "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_UPI0002FAB5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F43A718", "UniRef:UniRef50_UPI0002FAB5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002FAB5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FAB5C6" ], "product": "AAA domain-containing protein" } }, "id": "AHCKFKLKGB_530", "locus": "GCA013367975_00514" }, { "type": "cds", "sequence": "contig_1", "start": 294892, "stop": 295329, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGCCTACGTTAAATTCGACAATATACAATGATGAATTAGAAATTTTAAAGAAAGAATATTCAAAAGTTAGAAATAAAAAACCGATTAGAAAACTATTTAAAGAAATTCCAAATTTAATTCAAAATATTAAGCCTTGTTTTATGATGTCTCCCTCTTCGCTAAGTACATATTTAGCGGATAGTAGCATCACATTTGATAGTGTAATTTTTGATGAGGCTTCTCAATTAACGACAGAAAATGCCTTGATATCAATGATTAAGGCTAAACAAATTATTATTGCTGGTGATATAAATCAATTGCCACCAACTAATTTCTTTAAAACTATGTCTAGCGAGCAAGAACCAAACGAAGACGAAGAAATAGTCAACGAAGAAAATGATTATGATTCACTTCTTGAAAGAATGCAAAGCTCATTTAGACAAGTTACATTAAAATGA", "aa": "MPTLNSTIYNDELEILKKEYSKVRNKKPIRKLFKEIPNLIQNIKPCFMMSPSSLSTYLADSSITFDSVIFDEASQLTTENALISMIKAKQIIIAGDINQLPPTNFFKTMSSEQEPNEDEEIVNEENDYDSLLERMQSSFRQVTLK", "aa_hexdigest": "ec16b0df06247633108957fce7a498fc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 52, "aa_cov": 0.35172413793103446, "hmm_cov": 0.9807692307692307, "evalue": 7.97235970290909e-12, "score": 40.750946044921875, "start": 54, "stop": 104 } ], "seq_stats": { "molecular_weight": 16691.853299999988, "isoelectric_point": 4.7559118270874015 }, "id": "AHCKFKLKGB_531", "locus": "GCA013367975_00515" }, { "type": "cds", "sequence": "contig_1", "start": 295432, "stop": 296130, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATCTCGAAATAATATTTTTAAGCGTCTTTTTTCAAAGAGATATAGAGCAATAAAAAAAGAATTAAAAATTAATTTTATATCTTCTAATTTAAATTACAAAAACTTGTTAAAAGATTTAGTGGCATTAAACAACATTCAACTTGCTAAAAATGCAATTACGCTTTCAACAAATAAAATTCCTTTTTCATTTAAAGAAAACTATAATGAAATGGATCTTAATAGTTTAAAGCAACAACTAAATACATTTTCTAAATACGAAATTATTAATAATCAATTAAGTGAATTAAAATTTGATCAAAATTTAAAAATTAGCTTTTTTAACTTTTTATTAAGCCATATTCAATTAACAAATAATTACATAAATTTATATAAAAATTCAATGACTTTTGAAGATAAATTCAATGACTACTTTAACAATAAATATGTAGATTATTCAAATTCAAATTATAAAGTTAATGATTTTATAAAAAAACTAAATTTTTTAATTGATTCTGATGAATATCCTGAAGCATATTGTAGTTATATAAAATCAAGACAAGAATTAAAAAACAATCCCTTTACTAATGATTTTGTGGATGCTATTGAATTGCTAGATATTGATTGTTTAAAGCAAAATAGTATTGAAAATATTTTTAATAAAAGATTTTATCAACTTTTAATTGACTACTATTTATCAATTGATTTAGTAGATTGA", "aa": "MKSRNNIFKRLFSKRYRAIKKELKINFISSNLNYKNLLKDLVALNNIQLAKNAITLSTNKIPFSFKENYNEMDLNSLKQQLNTFSKYEIINNQLSELKFDQNLKISFFNFLLSHIQLTNNYINLYKNSMTFEDKFNDYFNNKYVDYSNSNYKVNDFIKKLNFLIDSDEYPEAYCSYIKSRQELKNNPFTNDFVDAIELLDIDCLKQNSIENIFNKRFYQLLIDYYLSIDLVD", "aa_hexdigest": "b853b6bd61cf9675dd9b81ff6b9aed00", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27844.369800000037, "isoelectric_point": 8.76907024383545 }, "id": "AHCKFKLKGB_532", "locus": "GCA013367975_00516" }, { "type": "cds", "sequence": "contig_1", "start": 296191, "stop": 296487, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGTAAAGCTTCAAGATTTAGAAAACATTAAAGAGATTTCTAGCCATTTAATTAATGAAATTCAAAGCCTAATCAATTTCTTAAATCTTGAAATTCCAAACAATTATCAACCATATATTAAAAATAGCAATTATTCATTATTTAATTCATTTTTTAAAATTTTTTCAAATATACAAGATATAGATATAAATCCAAAATATTTAGAAATTTTTTCGTATTATGAAACAAATGCAACACAAACCCTAAATGAAATACTATTGCAAAAAGAAAGAATTCAACCTGATATTGACTGA", "aa": "MDVKLQDLENIKEISSHLINEIQSLINFLNLEIPNNYQPYIKNSNYSLFNSFFKIFSNIQDIDINPKYLEIFSYYETNATQTLNEILLQKERIQPDID", "aa_hexdigest": "aba8fe582578b65e320a2f1f26213277", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11678.024099999988, "isoelectric_point": 4.479219627380371 }, "id": "AHCKFKLKGB_533", "locus": "GCA013367975_00517" }, { "type": "cds", "sequence": "contig_1", "start": 296503, "stop": 297258, "strand": "-", "frame": 1, "gene": null, "product": "DUF4011 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114E65", "UniRef:UniRef90_UPI0024114E65" ], "nt": "ATGGACATTTATTTAGATGTTGATAAAAACAAAGATAATATCATTAATTCAGAAATTATGAATGCAATGACGGGGATTAACAATGTTTTTAGTTCTGAATATTTGTATTCTGAAGAAGAAGTTGATAATATAGTTTCTCCTAAAGGCTATTATCACTGCCTAATGACTAATTCTTCGCAAGAAGCAGCTATTCAATCAGCAATTAAAGGAAATAGTTTTATTATTCAAGGTCCTCCAGGAACTGGAAAGTCTCAAACTATAGCAAATATAATAAGTGAATTAATTGCAAGAAATAAAAAGGTTCTATTTGTTGCTGAAAAAAAGGCTGCTTTGGATGTTGTTTATAAATCAATCCAAAATTTAGGATTTGCTAATTTTATTTTACTTATTCATAGCAATAACAGCAATAAAAAAGATTTTTCTAATGATTTGTATGAAACATTGCAAGACGGTCAAAAATTTAAAAAAGTAAGTGATGAAGTTATTGCTAATAATGATTATTTATATAGTGATTCATTAGAAAAAATGAATGATTTTGTTAGAAAGATATTATCAAATAGAAAACCACTAGGTCGCTCATTATATTTAATGATTGGTGATTATCAAAAATTACTTGCCAATACAAAAGGTATAAATTTTGATATTAAAGATTTTGAAAAAATTGATAATGCACAGTTTGTTGAAATTCAAAGTAGTTTAAATAAGTTTAATTTAAACTATAAAAATATAAATTTTAACGCTAAGGAAAGTTTATGA", "aa": "MDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEYLYSEEEVDNIVSPKGYYHCLMTNSSQEAAIQSAIKGNSFIIQGPPGTGKSQTIANIISELIARNKKVLFVAEKKAALDVVYKSIQNLGFANFILLIHSNNSNKKDFSNDLYETLQDGQKFKKVSDEVIANNDYLYSDSLEKMNDFVRKILSNRKPLGRSLYLMIGDYQKLLANTKGINFDIKDFEKIDNAQFVEIQSSLNKFNLNYKNINFNAKESL", "aa_hexdigest": "fa10e1cdf4d34ccc3fdc461ae1f0bb99", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 296200, "stop": 297558, "observations": { "mutation": [ 296505, 297306 ], "stop": [ 296505, 297306 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024114E65", "query_cov": 1.0, "subject_cov": 0.4009584664536741, "identity": 0.976, "score": 477.0, "evalue": 2.3e-164, "gene_start": 1, "gene_end": 251, "reference_start": 229, "reference_end": 479, "reference_sequence": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSLLQRKQKTLLEEQGINILYLAFGGLQWKDKTEKNVYSPLLFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNAEGSLYDRYSQYIEFINQKINSFNDSIEQNWKIVDEASLSAFRFSSMDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEFLYSEEEVDNIVSPKGYYHCLMTNSSQEAAIQSAIKGNSFIIQGPPGTGKSQTIANIISELIARNKKILFVAEKKAALDVVYKSIQNLGFANFILLIHSNNSNKKDFSNDLYETLQDGQKFKKVSDEVIANNDYLYSDSLEKMNDFVRKILSNRKPLGRSLYLMIGDYQKLLANTKGINFDIRDFEKIDNEQFVKIQSSLNRFNLNYKNINFNAKESLWYGLSILDVKLQDLENIKEISSHLINEIQSLINFLDLEIPNNYQPYIKNSNYSLFNSFFKIFSNIQDIDINPKYLEIFSYNKTNATQTLNEILLQKEKIQPDIDWILSRYNQNIFNSEIYKNLDFVKSRNNIFKRIFSKRYRAIKKN" }, "paralog": false, "identity": 0.9690265486725663, "score": 861.0, "evalue": 6.35e-318, "description": "Internal stop codon at 296505, 297306. Nonsense mutation around 296505, 297306.", "psc": { "uniref90_id": "UniRef90_UPI0024114E65", "product": "DUF4011 domain-containing protein", "uniref50_id": "UniRef50_UPI0024114E65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024114E65", "UniRef:UniRef50_UPI0024114E65" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024114E65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114E65" ], "product": "DUF4011 domain-containing protein" } }, "id": "AHCKFKLKGB_534", "locus": "GCA013367975_00518" }, { "type": "cds", "sequence": "contig_1", "start": 297304, "stop": 297546, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTACCTGTTTCAATTGAAAGCGAAAATGGATTGCAAAATAAAAAAATAATTATTCAAAGCAATGACTTTGTTGTAAATAATTCATTGATAAAGAAATTAAATGATGATTTTGGACTTGATATTAAATTTGAAGCATTTGATAATGCCGAAGGTTCTCTATATGATAGATATTCACAATACATTGAGTTTATAAATCAAAAAATAAATTCGTTCAATGATTCTATAGAACAAGATTGA", "aa": "MFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNAEGSLYDRYSQYIEFINQKINSFNDSIEQD", "aa_hexdigest": "66340d431ac70ac4c2e9c76663691d35", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9286.180099999996, "isoelectric_point": 4.303757667541505 }, "id": "AHCKFKLKGB_535", "locus": "GCA013367975_00519" }, { "type": "cds", "sequence": "contig_1", "start": 297583, "stop": 297942, "strand": "-", "frame": 1, "gene": null, "product": "DUF4011 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BA0F4", "UniRef:UniRef90_UPI00280BA0F4" ], "nt": "ATGGAAAATAAAGAATTAATTACAAAAACTTTGGCATATGAGTCTTTAAAGAATAAGCTATTAGACACAACATTAAGAAATATATTGATAAATTATAAAAAAAATAAATCATCAACTATAACAATTGAAAAGGAAGACATAAAATCAATTTTAGATAAAATTGAAAATAGAAGTGAATTTAAATTTATTGGTTCTCAAGATTCTGATGATGACATTGAAGTTGAAAGCAATGAATTTTTGTCCTCTGCTTCTCAAAAAGAATTATTAGCAATATTGTCGTTATTGCAAAGAAAGCAAAAGACTTTATTAGAAGAACAAGGAATTAATATTCTATATTTGGCTTTTGGTGGGCTTCATTGA", "aa": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSLLQRKQKTLLEEQGINILYLAFGGLH", "aa_hexdigest": "794a923b3160f7aba8e7a982848a9a41", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 297280, "stop": 297942, "observations": { "mutation": [ 297306, 297585 ], "stop": [ 297306, 297585 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280BA0F4", "query_cov": 0.9915966386554622, "subject_cov": 0.44360902255639095, "identity": 0.992, "score": 209.0, "evalue": 2.72e-66, "gene_start": 1, "gene_end": 118, "reference_start": 1, "reference_end": 118, "reference_sequence": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSLLQRKQKTLLEEQGINILYLSFGGLQWKDKTEKNVYSPLLFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNAEGSLYDRYSQYIEFINQKINSFNDSIEQDWKIIDEASLSAFRFSSMDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEYLYSE" }, "paralog": false, "identity": 0.9818181818181818, "score": 412.0, "evalue": 1.01e-148, "description": "Internal stop codon at 297306, 297585. Nonsense mutation around 297306, 297585.", "psc": { "uniref90_id": "UniRef90_UPI00280BA0F4", "product": "DUF4011 domain-containing protein", "uniref50_id": "UniRef50_UPI00280BA0F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280BA0F4", "UniRef:UniRef50_UPI00280BA0F4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00280BA0F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BA0F4" ], "product": "DUF4011 domain-containing protein" } }, "id": "AHCKFKLKGB_536", "locus": "GCA013367975_00520" }, { "type": "cds", "sequence": "contig_1", "start": 298183, "stop": 298914, "strand": "-", "frame": 1, "gene": "tpiA", "product": "Triosephosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "KEGG:K01803", "RefSeq:WP_036432897.1", "SO:0001217", "UniParc:UPI0005138FF4", "UniRef:UniRef100_A0A454C9S4", "UniRef:UniRef50_D1J827", "UniRef:UniRef90_D1J827" ], "nt": "ATGAAATATTTAATTGGTAATTTAAAAATGAATCTATCTTATTTAGAAAGTCAAAATTATTTAAATGAACTACAATCATTAAATAAAATTAAAAATTTTAAAAATGTAATAGTTGGAGCGGCTTTGTCATTCGATGCAATGTCTCTGTCTTTACAATATAATAAAAGAAATTTCTTGTTTGGAGTTCAAAATATTTTTTATAAAGAAAAAGGTGCATACACTGGGGAAGTTTCATTTAGATCAGCAGTTGAATTGAACATTGACTTTGTTTTAATAGGACATAGTGAACGTAGAAAATTATTTAATGAAACTGATCAATTGATTAATTTAAAAATTAAACAAATTGAAAAAACTAGCATTATTCCTATTTTGTGTATAGGGGAAGATCAAGAAAATTGTAGCGATAAAGTCTTTGAAGATATTATAAAAAACCAAATATTAAAAGCTTTAGAAGACGTCGATGTTTATAATCGTTTAATTATTTCATACGAACCTACTTATTGCATTGGAAATGGAAAAATTCCTCAAAATAACCATATCGAAAAAGCAATATCTGTAATTAAAAAAATTACTAATAATCAAGTTCCAGTGTTGTATGGTGGTTCAGTTTCACAAAAAAATATTGAAAAATTGCTTGAAATTAAGGATTTGGATGGTTTTTTAGTTGGCGGAGCAGCATTAGATGCTCAAGGTTTTTTAGAAATGGGTTCAATAATAGAAAAAAGCAACTAG", "aa": "MKYLIGNLKMNLSYLESQNYLNELQSLNKIKNFKNVIVGAALSFDAMSLSLQYNKRNFLFGVQNIFYKEKGAYTGEVSFRSAVELNIDFVLIGHSERRKLFNETDQLINLKIKQIEKTSIIPILCIGEDQENCSDKVFEDIIKNQILKALEDVDVYNRLIISYEPTYCIGNGKIPQNNHIEKAISVIKKITNNQVPVLYGGSVSQKNIEKLLEIKDLDGFLVGGAALDAQGFLEMGSIIEKSN", "aa_hexdigest": "f072e141c61a95602ea3d9cf508ba5ec", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0005138FF4", "ncbi_nrp_id": "WP_036432897.1", "uniref100_id": "UniRef100_A0A454C9S4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005138FF4", "RefSeq:WP_036432897.1", "UniRef:UniRef100_A0A454C9S4" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9S4", "uniref90_id": "UniRef90_D1J827" }, "psc": { "uniref90_id": "UniRef90_D1J827", "gene": "TpiA", "product": "Triosephosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_D1J827", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803" }, "pscc": { "uniref50_id": "UniRef50_D1J827", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J827" ], "product": "Triosephosphate isomerase" }, "genes": [ "tpiA" ], "id": "AHCKFKLKGB_537", "locus": "GCA013367975_00521" }, { "type": "cds", "sequence": "contig_1", "start": 298932, "stop": 299150, "strand": "-", "frame": 2, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J828", "UniRef:UniRef90_D1J828" ], "nt": "ATGCGTCGCGCAAAAGACAAATTTTATTCAGGAATGACAATTCATCAAAAGGATGTTATTATTGCTAAAGAACGCCAAGAATTTGAACGCGAAAGACTCAAAGCTGAAAAGCAAAGAAACTATCAATCAATTAATAATTTATCATTTCTTTTATTAATATTATATGGATTAATTATACTAATTATATTGATTCCATTTTTAGCATTAAAAATAGTTTGA", "aa": "MRRAKDKFYSGMTIHQKDVIIAKERQEFERERLKAEKQRNYQSINNLSFLLLILYGLIILIILIPFLALKIV", "aa_hexdigest": "5775a137310fe2c7a9390f485f205035", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 298932, "stop": 299450, "observations": { "mutation": [ 299195, 299390, 299402 ], "stop": [ 299195, 299390, 299402 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J828", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 0.972, "score": 123.0, "evalue": 3.4e-34, "gene_start": 1, "gene_end": 72, "reference_start": 102, "reference_end": 173, "reference_sequence": "MTLKQKNFRNQKKSISYWKNAWNKATISYFFVSLVIYIALIFIVRYSKKSIDGQYVHSWQNSLTVSMIFAITINFIIVVYRKGMGKWIVNPIANLIRNRIIMRRAKDKFYSGMTIHQKDIIIAKERQEFERERLKAEKQRNYQSINNLSFLLLILYGLIILIILIPFLALRIVW" }, "paralog": false, "identity": 0.9651162790697675, "score": 319.0, "evalue": 6.18e-114, "description": "Internal stop codon at 299195, 299390, 299402. Nonsense mutation around 299195, 299390, 299402.", "psc": { "uniref90_id": "UniRef90_D1J828", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_D1J828", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J828", "UniRef:UniRef50_D1J828" ] }, "pscc": { "uniref50_id": "UniRef50_D1J828", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J828" ], "product": "DUF3899 domain-containing protein" } }, "id": "AHCKFKLKGB_538", "locus": "GCA013367975_00522" }, { "type": "cds", "sequence": "contig_1", "start": 299193, "stop": 299351, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTATATTGCATTAATTTTTATAGTTAGATATTCAAAAAAATCTGTTGATGGTCAATATGTTCATTCTTGGCAAAATTCATTGACTGTATCAATGATTTTTGCAATTACTATAAATTTTATAATTGTTGTATATAGAAAAGGAATGGGAAAATGA", "aa": "MIYIALIFIVRYSKKSVDGQYVHSWQNSLTVSMIFAITINFIIVVYRKGMGK", "aa_hexdigest": "fe5ef27d68fb9bed388bcaf54785a2dc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6044.201899999998, "isoelectric_point": 9.9959680557251 }, "id": "AHCKFKLKGB_539", "locus": "GCA013367975_00523" }, { "type": "cds", "sequence": "contig_1", "start": 299572, "stop": 299775, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTGATGCAGAAATTAATTCAAATAAAATATTTCAAAAACCAGATGGCCAAGATTTTATTGAAAAATTTATAAACCCATATATTAATAAATTAGTTAATAACGAAAAAGACAGTTCATATACAAATTTCGAACAAGTTGAGCATGATAAAAGAGAATTTAACGAAATTTATCACAAAAAAATAAATTCACTAATCAAATAA", "aa": "MTDAEINSNKIFQKPDGQDFIEKFINPYINKLVNNEKDSSYTNFEQVEHDKREFNEIYHKKINSLIK", "aa_hexdigest": "b7035f2b2cbde89bd7ffd627bb2eeafb", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8051.8754999999965, "isoelectric_point": 5.5921838760375975 }, "id": "AHCKFKLKGB_540", "locus": "GCA013367975_00524" }, { "type": "cds", "sequence": "contig_1", "start": 299791, "stop": 300666, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9N2", "UniRef:UniRef90_A0A454C9N2" ], "nt": "ATGAGAAAAAAATTACTTTATATATTACCGTTATCTTCAACTATTTTAATAACCCCATTAGTTGCAGCTTCTTGTTCTAAAAAACCTAAACAAAATATAGAAACCACACAAACTAAAGATGAAAATGCACAAAAATCTGAAGAATTATTAAAAAAATTTAATGATGCAAAAACTGATCTTTTTAGTTATTTAAAAGATAACTTTTTGGTAAAAGGAGCTGAACAATTTAACACCTTAAAAGAAGTTCAAGATGCCAAGAAATTATTGGAATCTTTTAATAAAGAATCCCCTGAATCATCAATAGAAAGTTTAAATGAAAAAATACAAAAAATAAACGTTGCTAAACAAAAAATACTTAAAATTCAACCACTTTTAAAAGACTTAACAAAAATTGATAATTTAGTAAACGAATTAAATAATCTTGAAAATAAAATTAAATTAGATGCAGACAATTCTAAAATAGATAAATTAGTAAGAAACGAATTAAAATCAATTGCTGATAATTTATCTTTAACTTTAAAGGCAATTAATCTAGAAACATTATTGAATAATATTAATAGCAAAGATATTGCCAGTTATTTAAATAAATTGAACGAGATTAAGCAAATAACCTCGCTTAGAATTCAAAAAACTAAATTTATTAATTTAGCATTGCAACAAAAATTCCAAGACCAAGTAATTGAAAATATTATCAATAAAATTGCTAAAAACAATTTAAAAGAAAAATATATACCATTAATAAATCAATATCAATATTTTAAGGGGCAAATTATTGAAAAAATTAACAAATGTCATGCAAATGATAATTTAACAGAATCTTTAAATGAACAAATTAATTCTATAAGTCAAAAGTTAGCATTAAACGAGTCTATTTGA", "aa": "MRKKLLYILPLSSTILITPLVAASCSKKPKQNIETTQTKDENAQKSEELLKKFNDAKTDLFSYLKDNFLVKGAEQFNTLKEVQDAKKLLESFNKESPESSIESLNEKIQKINVAKQKILKIQPLLKDLTKIDNLVNELNNLENKIKLDADNSKIDKLVRNELKSIADNLSLTLKAINLETLLNNINSKDIASYLNKLNEIKQITSLRIQKTKFINLALQQKFQDQVIENIINKIAKNNLKEKYIPLINQYQYFKGQIIEKINKCHANDNLTESLNEQINSISQKLALNESI", "aa_hexdigest": "85faa53afaeeb4d4ae3dfb25d810f629", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A454C9N2", "query_cov": 1.0103092783505154, "subject_cov": 0.8010899182561307, "identity": 0.932, "score": 432.0, "evalue": 8.15e-150, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A454C9N2" }, "pscc": { "uniref50_id": "UniRef50_A0A454C9N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9N2" ], "product": "Lipoprotein" }, "genes": [], "id": "AHCKFKLKGB_541", "locus": "GCA013367975_00525" }, { "type": "cds", "sequence": "contig_1", "start": 300879, "stop": 301502, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175933469.1", "SO:0001217", "UniParc:UPI0015895F60", "UniRef:UniRef100_UPI0015895F60", "UniRef:UniRef50_D1J831", "UniRef:UniRef90_D1J831" ], "nt": "ATGAGAAATAAAATTCGCTTATTAATACTAAGTGCCGTTGCAACTTCAGTTGTTGCAATGCCATTAGTTGCAGCAGGATGCAAAACAACAGATAATAGTCAGAATTCTACAGCCACAGGTGAAGATTCGAGTGGACAATCTCAATCTGGCGACATTATAGATCCTAATAAGAGAGCAAAAGGTCAATTAGGCCAAAGCCAATTGTCATATATTAGAGACCAATTTGGTTTTACTCTAACTAATGAAGGTAAAGAAATATTAAATGACCCTGCACGTGGAACAAAAGAATTACAAAGAATTTTAGAAGGAATAATCAATAAGTATAAATCTATTGATGAACAAATTGAAAACAACATAAGAAATAAACAAACTAGTGGAATAAATTCGCAAGTTTATAAAGCATATAAAAATGTAATAAATGATGCAGATTTTGTAAAATATTTTAAGGTTACATTGCCTAAGATACTTATTCCGTTTGGCCACCCCATTTCAATACAATTAAAATCAAAGGGAAGATTAAGGGATTTGCCAGTAATTTCTTATAGTGTAATATGCCCTGATAAATCAAATAATGGAATTTTTAGTAGTGACGGTGATAGCGAAGTACCACTTGAAATTGATTAA", "aa": "MRNKIRLLILSAVATSVVAMPLVAAGCKTTDNSQNSTATGEDSSGQSQSGDIIDPNKRAKGQLGQSQLSYIRDQFGFTLTNEGKEILNDPARGTKELQRILEGIINKYKSIDEQIENNIRNKQTSGINSQVYKAYKNVINDADFVKYFKVTLPKILIPFGHPISIQLKSKGRLRDLPVISYSVICPDKSNNGIFSSDGDSEVPLEID", "aa_hexdigest": "d4d39a3ae049c9087dafe5580f4a7ee9", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0015895F60", "ncbi_nrp_id": "WP_175933469.1", "uniref100_id": "UniRef100_UPI0015895F60", "db_xrefs": [ "SO:0001217", "UniParc:UPI0015895F60", "RefSeq:WP_175933469.1", "UniRef:UniRef100_UPI0015895F60" ] }, "ips": { "uniref100_id": "UniRef100_UPI0015895F60", "uniref90_id": "UniRef90_D1J831" }, "psc": { "uniref90_id": "UniRef90_D1J831", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J831" }, "pscc": { "uniref50_id": "UniRef50_D1J831", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J831" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "AHCKFKLKGB_542", "locus": "GCA013367975_00526" }, { "type": "cds", "sequence": "contig_1", "start": 301504, "stop": 302052, "strand": "-", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175933470.1", "SO:0001217", "UniParc:UPI00158E13DA", "UniRef:UniRef100_UPI00158E13DA", "UniRef:UniRef50_D1J832", "UniRef:UniRef90_D1J832" ], "nt": "ATGAATAAAAAGATTACACTATTTTGTTTACCAATAGTTTTAATAATTCCAACAATTGCAGCAGTTTCATGCCAAAAATCCTCTAATTTAAAAACCAAAAATGATTTATTAAAAACCAAGATTATTGAAGGAATTAATTGCCCATGTCATACTAAATATAATGTTTTATCTAATAATAATCAAAACATGTCTAGTACTATGCTTGCAGCAATTTATGATAGTTTTAATTTTGAATCTAAAATACCGCTTAATCAAGAGAATAAGAAATTTTTATTCAAAATCATTGATAAACTCAATGATAAATACAAAAAAGATGATCAAAAGTCAAACATATTCAGTGATGAGTTATTTTTAAAATATTTTAAAATTTCATTGCCTGATTCGAAATACTTATTAAATCATACATTAAAATTAGAACTTGGATATGGTGATACAAATTTAAAATCAGATACTATATTTATAAAATATAGTATAATATGTAAATTAAGAAGTAGCCAATTTTTACAAGTTATTGAAGATGAACGATATATTTATATTCAGGGGGAATAA", "aa": "MNKKITLFCLPIVLIIPTIAAVSCQKSSNLKTKNDLLKTKIIEGINCPCHTKYNVLSNNNQNMSSTMLAAIYDSFNFESKIPLNQENKKFLFKIIDKLNDKYKKDDQKSNIFSDELFLKYFKISLPDSKYLLNHTLKLELGYGDTNLKSDTIFIKYSIICKLRSSQFLQVIEDERYIYIQGE", "aa_hexdigest": "263fa7baec443a87a2b8841525a280ae", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158E13DA", "ncbi_nrp_id": "WP_175933470.1", "uniref100_id": "UniRef100_UPI00158E13DA", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E13DA", "RefSeq:WP_175933470.1", "UniRef:UniRef100_UPI00158E13DA" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E13DA", "uniref90_id": "UniRef90_D1J832" }, "psc": { "uniref90_id": "UniRef90_D1J832", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J832" }, "pscc": { "uniref50_id": "UniRef50_D1J832", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J832" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "AHCKFKLKGB_543", "locus": "GCA013367975_00527" }, { "type": "cds", "sequence": "contig_1", "start": 302052, "stop": 302555, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_012855523.1", "SO:0001217", "UniParc:UPI0001BC21FB", "UniRef:UniRef100_D1J833", "UniRef:UniRef50_D1J833", "UniRef:UniRef90_D1J833" ], "nt": "ATGAAAAAAATAATATTATCTATAGGGGCAGTTCTAGTTTTAGCTTCGCCAATAATTTCAGCATCGTGTGTTTCAACAGATGAAAATACAGCAAAAGCAAATGAAATTTACACTAATAAAAGTGGAATTTTTAATTCTTCTCAATTAGAAGCAATCAAAAATGATTTTGTTTTCGAACTAACTGAACAGAGCAAAATACTAAAAAATAATTATGGCAATAAGGCGTTGGCAGATGAGCTAAAAAAGATTTGTAAAGATTATGAATTGCAAATTAATCTTAGCCCTTCTGAAAATAATAAATTAGCAGGTTTGAGGTTAATTAATAATGCAAACTTTTTAAAATTGTTTAAAGTGGTTAAGCCGAATTTAGGTGTAAATCACCAATTGATAATTAATTTTAAAATTGATAATAATAACAATATTTCATTAATCTATGACATATATTGCAAAGATGTAAAAACATATGATGCCAAAGATCAAGAAATAAAATTAGATTTAGAATAA", "aa": "MKKIILSIGAVLVLASPIISASCVSTDENTAKANEIYTNKSGIFNSSQLEAIKNDFVFELTEQSKILKNNYGNKALADELKKICKDYELQINLSPSENNKLAGLRLINNANFLKLFKVVKPNLGVNHQLIINFKIDNNNNISLIYDIYCKDVKTYDAKDQEIKLDLE", "aa_hexdigest": "c0e3d2396ac023e414bd2846a3e2afb3", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC21FB", "ncbi_nrp_id": "WP_012855523.1", "uniref100_id": "UniRef100_D1J833", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21FB", "RefSeq:WP_012855523.1", "UniRef:UniRef100_D1J833" ] }, "ips": { "uniref100_id": "UniRef100_D1J833", "uniref90_id": "UniRef90_D1J833" }, "psc": { "uniref90_id": "UniRef90_D1J833", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J833" }, "pscc": { "uniref50_id": "UniRef50_D1J833", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J833" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "AHCKFKLKGB_544", "locus": "GCA013367975_00528" }, { "type": "cds", "sequence": "contig_1", "start": 302565, "stop": 303413, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J834", "UniRef:UniRef90_D1J834" ], "nt": "ATGGTAAACATTTTTAAGCCATTGATTCCAAAAAATGTTCATGAAAATAGTCTTGACTTTATTGACAATAGTTATTATCTTGCAAACAAAAAAATATTAAACGATCTTCAATCAAGCAATCTTGATTACAAATTAAAATCAGCTTATTTTCAAGAAATATCTAAACAACTAAATGATATAATCAAAAAGTTTTATGAACAACATCCAGATGCTGCTGATATTGAATTTGATATTCCACTAAATTTTTTGAATTTGCAATTTAATAACCAAAGGCAAAATGTTTTGGAACATATCAAAACAATATTTAAAGCAGTAAATAAACATTTAAAGCCAAATTTTGTAGTTATTGATGATTTAGCAAAATATGACTTGTATTTTAATCAAGATGCTTCAATATATTGCAATAATAGCTTTGAACTTTTAAAATCAAATACTAAAGAATTTATAGCAACAGCCATTGCTAAAAACTATAAATTGTTTTTAATCTTTAATAAAGTTTCTAGCTTAACTAAAAGCAATAGTTTTATTCTAGAAAATACCAAAAAAATATTAAATGAATTAAATAAAACAAAACTAATTACAAATAATGAAATTTTTAACAAAAATTTAGAAGATTACAACAAAAATTTCTCTATAATAAAATCAATTATATTAAAATACTTAGAAAATATCTCTTACCAAGACCAATTAAATATAATTAATGATATTAACAGTTTACTTTCTTATACCTTTTCATTTGAAAATATCATTAAAACTGTTGGTTTTTCAAAAGTAATATACCAAAAATTTATTAATAAACCAATTAGTTATGACGGGTTGTCTTACTTGCAAGATATTGTAATAGAATAG", "aa": "MVNIFKPLIPKNVHENSLDFIDNSYYLANKKILNDLQSSNLDYKLKSAYFQEISKQLNDIIKKFYEQHPDAADIEFDIPLNFLNLQFNNQRQNVLEHIKTIFKAVNKHLKPNFVVIDDLAKYDLYFNQDASIYCNNSFELLKSNTKEFIATAIAKNYKLFLIFNKVSSLTKSNSFILENTKKILNELNKTKLITNNEIFNKNLEDYNKNFSIIKSIILKYLENISYQDQLNIINDINSLLSYTFSFENIIKTVGFSKVIYQKFINKPISYDGLSYLQDIVIE", "aa_hexdigest": "c2ac32b787bd1e27d6f4722c51076f26", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 302565, "stop": 303713, "observations": { "mutation": [ 303491, 303680 ], "stop": [ 303491, 303680 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J834", "query_cov": 1.0, "subject_cov": 0.6482758620689655, "identity": 0.993, "score": 541.0, "evalue": 7.95e-192, "gene_start": 1, "gene_end": 282, "reference_start": 154, "reference_end": 435, "reference_sequence": "MPISKNTFGMQMYHAFLQNIVTTLNFDDLDINQQQEILHKYNDFNISYTRNVSKNKLSNNITINWMPTANKKFFNDSFSMLAYGLKANQLNENTNINKILKSKNIAFLLLIHQLINQYGMLANQGDIWTSQAPSDLDISSFDSGINYHVLKDSMVNIFKPLIPKNVHENSLDFIDNSYYLANKKILNDLQSSNLDYKLKSAYFQEISKQLNDIIKKFYEQHPDAADIEFDIPLNFLNLQFNNQRQNVLEHIKTIFKAVNKHLKPNFVVIDDLAKYDLYFNQDASIYCNNSFELLKSNTKEFIATAIAKNYKLFLIFNKISSLTKSNSFILENTKKILNELNKTKLITNNEIFNKNLEDYNKNFFIIKSIILKYLENISYQDQLNIINDINSLLSYTFSFENIIKTVGFSKVIYQKFINKPISYDGLSYLQDIVIE" }, "paralog": false, "identity": 0.9895287958115183, "score": 744.0, "evalue": 3.48e-274, "description": "Internal stop codon at 303491, 303680. Nonsense mutation around 303491, 303680.", "psc": { "uniref90_id": "UniRef90_D1J834", "uniref50_id": "UniRef50_D1J834", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J834", "UniRef:UniRef50_D1J834" ] }, "pscc": { "uniref50_id": "UniRef50_D1J834", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J834" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 33089.53780000005, "isoelectric_point": 8.286137580871586 }, "id": "AHCKFKLKGB_545", "locus": "GCA013367975_00529" }, { "type": "cds", "sequence": "contig_1", "start": 303489, "stop": 303677, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAACTGCAAATAAAAAATTTTTTAATGATAGTTTTTCAATGCTAGCATATGGTTTAAAAGCAAACCAATTAAATGAAAATACGAATATAAATAAGATATTAAAATCTAAAAATATTGCCTTTTTATTATTGATTCACCAATTGATTAATCAATATGGGATGTTGGCTAACCAAGGTGATATTTGA", "aa": "MPTANKKFFNDSFSMLAYGLKANQLNENTNINKILKSKNIAFLLLIHQLINQYGMLANQGDI", "aa_hexdigest": "447ece77dc0a74eed10a91cb8d6d0fa3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7030.111899999999, "isoelectric_point": 9.523801612854005 }, "id": "AHCKFKLKGB_546", "locus": "GCA013367975_00530" }, { "type": "cds", "sequence": "contig_1", "start": 303678, "stop": 303872, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATATCAAAAAATACTTTTGGCATGCAAATGTATCATGCATTTTTGCAAAATATTGTAACTACATTGAATTTTGACGATCTTGACATAAATCAACAACAAGAAATTTTGCATAAATATAATGATTTTAATATAAGCTATACTCGCAATGTTTCTAAAAATAAGTTGAGTAATAATATTACTATTAATTGA", "aa": "MPISKNTFGMQMYHAFLQNIVTTLNFDDLDINQQQEILHKYNDFNISYTRNVSKNKLSNNITIN", "aa_hexdigest": "3364ab9bd125409e701de2e7bc69a378", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7553.4361, "isoelectric_point": 6.698270606994629 }, "id": "AHCKFKLKGB_547", "locus": "GCA013367975_00531" }, { "type": "cds", "sequence": "contig_1", "start": 304431, "stop": 304994, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAACATTCTTATTTTAAGTTCAATGTTAGCATTATTTACAATGACATCTGCGATATCAATATCTTGTAAATTTAATAATTTTAATGAAGATAAGAAAAAATATATTCAAAAGCTTAATGTTGCAAATCCTTATTTAGAATATGGCAGTTCAATACCAAATTTTAAAAACCAAAATTTAAAAGAAATAAATTTAATGACAGGAGCCAAAGTTCTTAGAATTTATTCAAAAAATCAACCTAAAATAGACTTTAGGGATAATATAGTTTTGCGCCCGACTGAATTAAGTTATAAATTTGAATATGGTAATAGTATTCAAATAAATGATAAACTTTTCAATAGCGATGATAAACTAAATGTTGACTATCAAAACACTGAATTGAATAAGCAAGATCCCAACAAATATTACCCCAGCAAAGACAAGGGCAATGGTTTTAATAATCCATATTTGTTCGTTCCTTCAAAAAATTCAATGTCTATAAATAGCAAACAATTCATTGAATCTCTAAGAAAAGCAAAAAATATTTCAATCAGTATAAATCAAAGAAAAAATTATTGA", "aa": "MKKNILILSSMLALFTMTSAISISCKFNNFNEDKKKYIQKLNVANPYLEYGSSIPNFKNQNLKEINLMTGAKVLRIYSKNQPKIDFRDNIVLRPTELSYKFEYGNSIQINDKLFNSDDKLNVDYQNTELNKQDPNKYYPSKDKGNGFNNPYLFVPSKNSMSINSKQFIESLRKAKNISISINQRKNY", "aa_hexdigest": "044b61f2915b3833d0edfc2195d746f2", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21753.645100000005, "isoelectric_point": 9.670080375671386 }, "id": "AHCKFKLKGB_548", "locus": "GCA013367975_00532" }, { "type": "cds", "sequence": "contig_1", "start": 305200, "stop": 305481, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATGAGTCATAGAATTATTGATGGACTATTTTTGGGATATAATGTTTATACATTAACATCAATTGGTGGAACATATAAAGCAAATATTCCTTTAATAATAACATTTAATATTCATATATTACTTGAAATTTTAGTTGTTTATTATCGTCAAATTCAATATGGAGAAAAGAAATCAAAAGCCATACTATACAACTTCTTAACATTTTTATTAATTATCCCATTTATGTTAATTGGCGGATTGCTTGGCCAATATATTGAACATAGTAAAGTGTTTTGA", "aa": "MLMSHRIIDGLFLGYNVYTLTSIGGTYKANIPLIITFNIHILLEILVVYYRQIQYGEKKSKAILYNFLTFLLIIPFMLIGGLLGQYIEHSKVF", "aa_hexdigest": "6c261c62e31d345c939dfab4a8d16dc6", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10745.78719999999, "isoelectric_point": 9.260512733459471 }, "id": "AHCKFKLKGB_549", "locus": "GCA013367975_00533" }, { "type": "cds", "sequence": "contig_1", "start": 305722, "stop": 305949, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATAGGAACAAGTGCATTTAACGGAAATATAACTTTAGCAACATTTGTAAATCTAATAATCTATATTTTAATATTATTGGGCGTACCTTCATTAGTTTTGGGCGTAATTTCTATATTTAAATTAAAAATTACTGATAAGAAAAATATTTATATTTATGCATTTGCTACGGGAATGTTTCTAATGATTGGCACTGTAGGTTTCTTTAAAGAAAGTTTTGATTGA", "aa": "MFIGTSAFNGNITLATFVNLIIYILILLGVPSLVLGVISIFKLKITDKKNIYIYAFATGMFLMIGTVGFFKESFD", "aa_hexdigest": "2dc2ffb6a1c8af9c24258720723a8558", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8289.930899999998, "isoelectric_point": 9.158330345153807 }, "id": "AHCKFKLKGB_550", "locus": "GCA013367975_00534" }, { "type": "cds", "sequence": "contig_1", "start": 306030, "stop": 306497, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAAGTTTTATTTTAATTCTATTTTAATTGCTAGCGCACTTAGCTTTATGCCTTTAATAATTTGTAGTTGTCAAACTAAAAAAGATAACTATATTATAGAGGGTAGCAATGCTTATATAGATATTTCAAAAATTTCTAGAGTTTTTTTACATAGGTTAAGCATAGGTCAGATTGCTGCATTACACAACATGTATAAGATTTTTTATTACTATGATAATTCTAATAAAAAGCAATATTATGACTCTGCATATGTAAATGGCCAAAAGCTATATTTAAGAAATGCAAATAAAAATGTTGAATATAAATTAAATTTTCCAATTAAACCTTCTTGGAAACAAGAAATATCAAAATTTGACAATATTAATATTGTAAAAAGCAATAAACCAAGCGATATTAATAATTTTTTAAACGAATATAGTTTTGATGAAGTAGATTCTGCAGGCGATGTTAATGACGAATGA", "aa": "MKKKFYFNSILIASALSFMPLIICSCQTKKDNYIIEGSNAYIDISKISRVFLHRLSIGQIAALHNMYKIFYYYDNSNKKQYYDSAYVNGQKLYLRNANKNVEYKLNFPIKPSWKQEISKFDNINIVKSNKPSDINNFLNEYSFDEVDSAGDVNDE", "aa_hexdigest": "866269285d23b10328405dc0bd6e2503", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18121.401499999993, "isoelectric_point": 8.886209297180176 }, "id": "AHCKFKLKGB_551", "locus": "GCA013367975_00535" }, { "type": "cds", "sequence": "contig_1", "start": 306564, "stop": 306707, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACTATTATTTTTAGATTAATTCAAGATACAGAATTAAATTATTCAATAATGAAATCTAAATATTTAATAAATTCTAAAAGAGAAAATGTAATTACACAAAATTTATTTAAAAACAAATACATTCAAGCTAGTTCATGA", "aa": "MQTIIFRLIQDTELNYSIMKSKYLINSKRENVITQNLFKNKYIQASS", "aa_hexdigest": "e03fbba843d8b202d0a18682185b7014", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5627.493300000002, "isoelectric_point": 9.775808525085449 }, "id": "AHCKFKLKGB_552", "locus": "GCA013367975_00536" }, { "type": "cds", "sequence": "contig_1", "start": 307143, "stop": 307565, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTTTTTCAAAGCTTCAAAAATGAACCTGGTTTCTTTAAATTAGCAGTTCCTGAATATAAAAAAAATGAAGATAAGGAATATAAAATTATTGATTTTGAATATACTCCATATTTTAAAACAAATCAAATTTTTAAAGCAATAGTAAGGGTATTTAAACTAAATGGTACATATAAAGATTATGTTTTAATATCTTCCAATTTTGATGATCACGGTCACAGATTAAAAGGTTTAATTACTAAAAATGCTTTGCCAAATGAACTTAAAGCTTCTGATATATATTCAATTAGAGCAGATAGTGAGCCAATTAAACAAGGAATCAAATTAGATGATTTCATTGATAAGAATAATAAAAATAGTGTTTTTAGATATATGTTAAATGAATCTGCAAAAAAACTAGAAAATATTTTTGAATATTGA", "aa": "MYLFQSFKNEPGFFKLAVPEYKKNEDKEYKIIDFEYTPYFKTNQIFKAIVRVFKLNGTYKDYVLISSNFDDHGHRLKGLITKNALPNELKASDIYSIRADSEPIKQGIKLDDFIDKNNKNSVFRYMLNESAKKLENIFEY", "aa_hexdigest": "334cd2664ba84e5911bb83b26f45ccc0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16604.766499999987, "isoelectric_point": 8.872735404968264 }, "id": "AHCKFKLKGB_553", "locus": "GCA013367975_00537" }, { "type": "cds", "sequence": "contig_1", "start": 307605, "stop": 307862, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGCCAAAGATCCATTCCAATTAAAATTACTAGCTTCTTATTTAAATAACTACTTATTAGCATATGCGCTTGAAAATAAGGCAGGTGAAGTATTTAGTGGAATTAAACGCATCGATCTTAACATAGTTAAATTACCAAATCAACCAATTGGAAGATTATATTTAAAGATGGATTTTGTTTCATATGCCAATAACCAAGATTTCAAATTCAAATCAAAAAATGAAAAAATTGTAAAAAGTGTTTATTTATATTGA", "aa": "MLAKDPFQLKLLASYLNNYLLAYALENKAGEVFSGIKRIDLNIVKLPNQPIGRLYLKMDFVSYANNQDFKFKSKNEKIVKSVYLY", "aa_hexdigest": "90a334c80318eee6e758a6d23630ff18", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9882.500599999992, "isoelectric_point": 9.58440189361572 }, "id": "AHCKFKLKGB_554", "locus": "GCA013367975_00538" }, { "type": "cds", "sequence": "contig_1", "start": 307946, "stop": 308323, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATCAATAAAATTGCTATCCTTGCTTTGTACTTCTACAATAATTTCATTGCCTGCTATTTCTTGCACTATCAAAGTAAATCATGATAACGAAGACAAGGAAAATTTAGATTTTTCAGATGATTTTAGTGGTAAAAAGCAAAAAAATGAATTCAATTTCAAAAATAAGATTTCCCAAAATATTTATGATTTGTATCAAAATAAAATTGCTATTTTATTTAACGATGTAAAAACTAATTACCGGGGATATAAAAAAATATTTGGATCTTTAACAAGAAACTTAAATACTCTAAGGGCTAAGATTAATAGCTTAAGATACGAACAAAGTTTAAAAGAAAACAAAAAAATATTAAATGATTTTTACAATAACTGA", "aa": "MKKSIKLLSLLCTSTIISLPAISCTIKVNHDNEDKENLDFSDDFSGKKQKNEFNFKNKISQNIYDLYQNKIAILFNDVKTNYRGYKKIFGSLTRNLNTLRAKINSLRYEQSLKENKKILNDFYNN", "aa_hexdigest": "57145e4268d4c466205f53ca91933e95", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14638.602699999981, "isoelectric_point": 9.562353706359861 }, "id": "AHCKFKLKGB_555", "locus": "GCA013367975_00539" }, { "type": "cds", "sequence": "contig_1", "start": 308342, "stop": 308539, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAAAATAATCCATTTGCATTATATTTATTCAAATATAGTTTAATTTTTTCAGATGTGGATGCTGTATTAGCTGATACAAATCTTGTTTTTGAAACTTCTGAATTTTTAAAATATTTAAAGACAATTGATGACAGACTTGAAGGTAAAGACATTGATCTAGGTCAAACTCAACATGCATTACAAGCGGTTTGA", "aa": "MLKNNPFALYLFKYSLIFSDVDAVLADTNLVFETSEFLKYLKTIDDRLEGKDIDLGQTQHALQAV", "aa_hexdigest": "53ee0f7517b2a8fe14d8b8609555d331", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7441.420799999998, "isoelectric_point": 4.562545585632326 }, "id": "AHCKFKLKGB_556", "locus": "GCA013367975_00540" }, { "type": "cds", "sequence": "contig_1", "start": 308591, "stop": 308830, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGATAAAATTAACATTGAAGATGATAAAAACTCGCATAATCACTCACATGCAATCATAAATTTAACATATGAAATGGGTTTATGGCATGAAATGCTAGCAAAAAATAAACTTAAAAATTACGAAGAATTTCAAAAAGAATATAAAATTGCTAAAAAACACATAATCGATAATATAAACAATATTGATGTAAATAATAATTATTTAGCATTAAAAAAAGTCCTAGATAAAGATTGA", "aa": "MLDKINIEDDKNSHNHSHAIINLTYEMGLWHEMLAKNKLKNYEEFQKEYKIAKKHIIDNINNIDVNNNYLALKKVLDKD", "aa_hexdigest": "75246a864e687229581d69ec09553e31", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9437.66269999999, "isoelectric_point": 6.501209831237793 }, "id": "AHCKFKLKGB_557", "locus": "GCA013367975_00541" }, { "type": "cds", "sequence": "contig_1", "start": 309280, "stop": 309618, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAATTTTTAGAAAATCTTCATTTAATTCACTTTATCATAAAGTTTTAACGATCATGTCTAATACACATTGTTCATTTGAAGAAAAAATGGAAACAATTAAAAAAGTTTTATTAAGCAAAGGAATTAATCTAGTAACGATGCCTTTTATTGAAAATAGTTTGATAAGGGCTATTGCTTTAAAAAAGGGAGCAAAAAGGTATATTTTCCTTTCTGATATGTTTAATTCTGAAGCATATATATTTTATTCATTGTTACATGAAATAGTTCATTGCTTTTTTTCAAATTATACTGAAAACAAAATTGATAAAGTTGTTATCAAAGAATATTTTGCATGA", "aa": "MGIFRKSSFNSLYHKVLTIMSNTHCSFEEKMETIKKVLLSKGINLVTMPFIENSLIRAIALKKGAKRYIFLSDMFNSEAYIFYSLLHEIVHCFFSNYTENKIDKVVIKEYFA", "aa_hexdigest": "77f1ae2d9b0245b3d766fd258cd43f37", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13119.326799999984, "isoelectric_point": 9.250778007507321 }, "id": "AHCKFKLKGB_558", "locus": "GCA013367975_00542" }, { "type": "cds", "sequence": "contig_1", "start": 309697, "stop": 310179, "strand": "-", "frame": 1, "gene": null, "product": "HTH cro/C1-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J844", "UniRef:UniRef90_D1J844" ], "nt": "ATGATTGAAGAGCACATCTGTGATATGAAGCACGTTAAAATTCATGGCAATGATTTTACAGATGAAATAAAATACTATTTAAAAATGTTTTCAATGACTCAAAAAGAATTATCTATAAGACTTGGATTATCAATAAAACATATCAATTCAATTATGAATAATGAAGTTAATAACGTTAGTGCAACTATTATTGAAGCGCTAGAATATGCATTTCACTTAGAAATTGGCACATTAACAGAAGTTTATCATATTTATAATAACCTAAAAACGTTAAAGAAAAATAAGAATATTGAAGATGATTTAAAGAAGCATGGTATTGATTTTTTAATAAATCATCCAGAGCTTGCATTGGCAGCTAACATTAGCATTCATGAAAATAGTCCATTACATGTTAAATTAATAATGCTAAAAAGATTTTTTGGTGTTGCTGATTTGGCTAACTATGATAAATATTTAAAAGATAATGTTTCGGCAGAAGAATGA", "aa": "MIEEHICDMKHVKIHGNDFTDEIKYYLKMFSMTQKELSIRLGLSIKHINSIMNNEVNNVSATIIEALEYAFHLEIGTLTEVYHIYNNLKTLKKNKNIEDDLKKHGIDFLINHPELALAANISIHENSPLHVKLIMLKRFFGVADLANYDKYLKDNVSAEE", "aa_hexdigest": "e07654f3e13b12c233b1754b5b25cdd6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 309394, "stop": 310179, "observations": { "mutation": [ 309669, 309699 ], "stop": [ 309669, 309699 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J844", "query_cov": 1.0, "subject_cov": 0.4456824512534819, "identity": 1.0, "score": 322.0, "evalue": 1.35e-108, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MIEEHICDMKHVKIHGNDFTDEIKYYLKMFSMTQKELSIRLGLSIKHINSIMNNEVNNVSATIIEALEYAFHLEIGTLTEVYHIYNNLKTLKKNKNIEDDLKKHGIDFLINHPELALAANISIHENSPLHVKLIMLKRFFGVADLANYDKYLKDNVSAEEWTYSNPNTKVWIRFCELLSIDKNIDENVGIFRKSSFNSLYHKILTIMSNTHCSFEEKMETIKKVLLSKGINLVTMPFIENSLIRAIALKKGAKRYIFLSDMFNSEAYIFYSLLHEIVHCFFSNYTENKIDKVVIKEYFAWEKNNPTTYKAIYDAINSYQQCEAIQKANKNIDTSYIWSTLKDKYPYVAFDPLSDQKSKN" }, "paralog": false, "identity": 0.9885057471264368, "score": 512.0, "evalue": 7.76e-186, "description": "Internal stop codon at 309669, 309699. Nonsense mutation around 309669, 309699.", "psc": { "uniref90_id": "UniRef90_D1J844", "product": "HTH cro/C1-type domain-containing protein", "uniref50_id": "UniRef50_D1J844", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J844", "UniRef:UniRef50_D1J844" ] }, "pscc": { "uniref50_id": "UniRef50_D1J844", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J844" ], "product": "HTH cro/C1-type domain-containing protein" } }, "id": "AHCKFKLKGB_559", "locus": "GCA013367975_00543" }, { "type": "cds", "sequence": "contig_1", "start": 310354, "stop": 310749, "strand": "-", "frame": 1, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0008270", "GO:0009972", "KEGG:K01489", "RefSeq:WP_036438513.1", "SO:0001217", "UniParc:UPI0005144531", "UniRef:UniRef100_A0A454C9K2", "UniRef:UniRef50_D5E4U5", "UniRef:UniRef90_A0A454C9K2" ], "nt": "ATGACAATAGAAGAATTAAAAAATAAATTAAAATATTCATTTAGTCCTTATTCACATGTTCAAGTTGCATGTTGTGCAATTGAAGAAAATGGTAAAGAATATTGGGGAGTCAATACTGAAAACGCTGCATTTCCATCTGGATTATGTGCTGAAAGAAGTGCATTATTTGGTTCTGTAGCATATGGCGCTAGAGTTGGTAATTTTAAAGAAATACATATAATTAGCAATTTGTCTAAATTGCTATATCCTTGCGGTGCTTGTGCTCAAGTTATTAGTCAATTTTTAAAACCAGATGGCGATGTATATTTATACAGTTTTGATTTAAAGCAACAAAAGCATTATAAAATGAAAGATATTCTACCTTTTGCAGTTGCACCAAAAGATATAGTGGATTAG", "aa": "MTIEELKNKLKYSFSPYSHVQVACCAIEENGKEYWGVNTENAAFPSGLCAERSALFGSVAYGARVGNFKEIHIISNLSKLLYPCGACAQVISQFLKPDGDVYLYSFDLKQQKHYKMKDILPFAVAPKDIVD", "aa_hexdigest": "675e8b3d310036b1f9d0a200199bd6cb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0005144531", "ncbi_nrp_id": "WP_036438513.1", "uniref100_id": "UniRef100_A0A454C9K2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005144531", "RefSeq:WP_036438513.1", "UniRef:UniRef100_A0A454C9K2" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9K2", "uniref90_id": "UniRef90_A0A454C9K2" }, "psc": { "uniref90_id": "UniRef90_A0A454C9K2", "gene": "Cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_D5E4U5", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0008270", "GO:0009972" ] }, "pscc": { "uniref50_id": "UniRef50_D5E4U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E4U5" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "AHCKFKLKGB_560", "locus": "GCA013367975_00544" }, { "type": "cds", "sequence": "contig_1", "start": 310901, "stop": 311158, "strand": "+", "frame": 2, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "RefSeq:WP_036432541.1", "SO:0001217", "UniParc:UPI000513536C", "UniRef:UniRef100_A0A2K9YTE6", "UniRef:UniRef50_B3PMA1", "UniRef:UniRef90_D1J846" ], "nt": "ATGGTTAAATTAAGATTAAAAAGAACTGGTAAAAAATTCTATGCAACATATAAAATTGTTGCCGCAGATGCAAGAAGCCCACGTGATGGTAAATTTATTGAAGAATTAGGCTACTATGATCCTAATTCAAAAAAATTAGTGTTAAATAAGGAATTAATTATTAAACACTTGAACAACGGTGCAAAACCTTCAGACACTGTACGTAATTTGTTAAAGCAAGACAACTTCTATGCAGACTATGTTGCTTCTAAAAAATAA", "aa": "MVKLRLKRTGKKFYATYKIVAADARSPRDGKFIEELGYYDPNSKKLVLNKELIIKHLNNGAKPSDTVRNLLKQDNFYADYVASKK", "aa_hexdigest": "a7a483f38af834dbe4316c42c22e2626", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000513536C", "ncbi_nrp_id": "WP_036432541.1", "uniref100_id": "UniRef100_A0A2K9YTE6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513536C", "RefSeq:WP_036432541.1", "UniRef:UniRef100_A0A2K9YTE6" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTE6", "uniref90_id": "UniRef90_D1J846" }, "psc": { "uniref90_id": "UniRef90_D1J846", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_B3PMA1", "cog_id": "COG0228", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMA1" ], "product": "Small ribosomal subunit protein bS16" }, "genes": [ "rpsP" ], "id": "AHCKFKLKGB_561", "locus": "GCA013367975_00545" }, { "type": "cds", "sequence": "contig_1", "start": 311171, "stop": 311773, "strand": "+", "frame": 2, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "db_xrefs": [ "COG:COG0336", "COG:J", "EC:2.1.1.228", "GO:0008033", "GO:0052906", "KEGG:K00554", "SO:0001217", "UniRef:UniRef50_A9NHF7", "UniRef:UniRef90_D1J847" ], "nt": "ATGAAGATAAACATTCTGACACTATTTCCAGATTATTTAAATGCTTTTTGCAATCAAAGTATGATAGCAAAAGCATTAGAGCTTGGACACATTGAAATAAACATCATTAATTTTAGAGATTATTCATTAGATAAACATCATAAAGTTGATGATAGTCCTTATGGTGGAGGCGCAGGTATGTTGTTGCAAATCGAACCAATAGATTTAGCACTGCAACCTTTAAAAAATAGTTACAAAATTTTACTTTCACCGCAAGGAAAGGTATTTAGTCAAAAAAAAGCACATGAACTTGCAAAATTAAATGAAATTACTTTAATATGTGGGCATTATGAAGGCTTTGATGAAAGAATAACAAAATTAGTAGATGAAGAAATATCAATTGGAGATTTTATCTTAACGGGTGGAGAAATTGCAGCAATGGCAATTTCAGAAGCAGTAGCAAGATTAATACCAGGAGTATTAAGAAAAAATAGCTATGAAAATGAAAGCTTCGAAGGAAATGGATTACTAGATTATCCACAATATACAAGACCTGCCGTATATAAGGGAATGAAAGTACCAAAAATCTTATTAAGTGGAAATCACCTAGAAATAGAAAAATGA", "aa": "MKINILTLFPDYLNAFCNQSMIAKALELGHIEINIINFRDYSLDKHHKVDDSPYGGGAGMLLQIEPIDLALQPLKNSYKILLSPQGKVFSQKKAHELAKLNEITLICGHYEGFDERITKLVDEEISIGDFILTGGEIAAMAISEAVARLIPGVLRKNSYENESFEGNGLLDYPQYTRPAVYKGMKVPKILLSGNHLEIEK", "aa_hexdigest": "15108877a695f1001c7ff64434b4bcc2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J847", "query_cov": 1.0, "subject_cov": 0.9009009009009009, "identity": 1.0, "score": 402.0, "evalue": 1.34e-141, "valid": true, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_A9NHF7", "cog_id": "COG0336", "cog_category": "J", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_A9NHF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A9NHF7" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [ "trmD" ], "id": "AHCKFKLKGB_562", "locus": "GCA013367975_00546" }, { "type": "cds", "sequence": "contig_1", "start": 311841, "stop": 312197, "strand": "+", "frame": 3, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "COG:COG0335", "COG:J", "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_012855536.1", "SO:0001217", "UniParc:UPI0001BC2235", "UniRef:UniRef100_D1J848", "UniRef:UniRef50_Q044E2", "UniRef:UniRef90_D1J848" ], "nt": "ATGAGAACAAAATTGTTAGAATTAGTAGAAAAAGATCAAATAAGAGAAGACTTGCCACAATTAAGAGAAGGTTATAACGTAAGAGTTCACGTAAGAATTAAAGAAGGCGATAAAGAAAGAATTCAAGTTTTTGAAGGATTAATTATTGCTGAATATGGCACAGGAATCAATAAATCAATTACCGTAAGAAAAGAATCATATGGTGTTGGAGTTGAAAGAATCTTTAAAATTAACTCACCTTTAATTGCTCAAATCGAAGTTATTAGACGTAATAAAGTAAGACGTGCTAAATTGTATTACATGAGAGATTTAAAAGGTAAGAGTGCTAGATTAAAAGAAATTAAAAACTCAAAATAA", "aa": "MRTKLLELVEKDQIREDLPQLREGYNVRVHVRIKEGDKERIQVFEGLIIAEYGTGINKSITVRKESYGVGVERIFKINSPLIAQIEVIRRNKVRRAKLYYMRDLKGKSARLKEIKNSK", "aa_hexdigest": "9c890839eeb918280e6bf122bb6ab676", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2235", "ncbi_nrp_id": "WP_012855536.1", "uniref100_id": "UniRef100_D1J848", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2235", "RefSeq:WP_012855536.1", "UniRef:UniRef100_D1J848" ] }, "ips": { "uniref100_id": "UniRef100_D1J848", "uniref90_id": "UniRef90_D1J848" }, "psc": { "uniref90_id": "UniRef90_D1J848", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_Q044E2", "cog_id": "COG0335", "cog_category": "J", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q044E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q044E2" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "AHCKFKLKGB_563", "locus": "GCA013367975_00547" }, { "type": "cds", "sequence": "contig_1", "start": 312408, "stop": 312692, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACACACAAAAATTTTATTAGCATTAGGAAGCATTGCAACTTCAGTTGCTGCAATACCATTAGTGGCAGCAGGTTGCGTAAAAACAAAAAAACCAGAAGATAAAAAATCAGGAACAACTCCAGAAGATCCTAAAAAATCAGAAGGTGAAAAGCCAGGAACAACACCTGAAGTTAAACCAGAAGATCCTAAAAAATCAGAAGGTGAAAAACCAGGAACAACACCTGAAGTTAAACCAGAGGATAAAAGAACAGAAGAACAAAAAGAAATAGATAATTGA", "aa": "MKKHTKILLALGSIATSVAAIPLVAAGCVKTKKPEDKKSGTTPEDPKKSEGEKPGTTPEVKPEDPKKSEGEKPGTTPEVKPEDKRTEEQKEIDN", "aa_hexdigest": "d431bccc7bed15775d2763e27cede9dd", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10090.366399999997, "isoelectric_point": 7.780447578430176 }, "id": "AHCKFKLKGB_564", "locus": "GCA013367975_00548" }, { "type": "cds", "sequence": "contig_1", "start": 312962, "stop": 313177, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTCTAGTTTAACATCATCGGCAGTTAATTTGTTAATTTCTTTGTTTTTGTCTTTGATATCAAATTTAAGATCTTTTATAATTTTATCTAATTGTGTTTCTGATAGATCTATTTCATTGCTTACAATTTTAGTTCCAATAATAGGATCTTTTCTTGCAACAAATTTAGCTCCTTTAAATTTTAATGTTATCTTATTGTCTTTTAATTCATAG", "aa": "MPSSLTSSAVNLLISLFLSLISNLRSFIILSNCVSDRSISLLTILVPIIGSFLATNLAPLNFNVILLSFNS", "aa_hexdigest": "16b4dd445e71ad257f99816ca7f01344", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7610.965999999997, "isoelectric_point": 7.999833488464356 }, "id": "AHCKFKLKGB_565", "locus": "GCA013367975_00549" }, { "type": "cds", "sequence": "contig_1", "start": 313476, "stop": 313583, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTATCTAAAAGGAAATCATAATATTATTATTGAATTTAATGGCCAAAAACCAAATGAAACCTTAAGTCAAGTTGTATTTGAAGGAAAATCCGGAGCATTTTGA", "aa": "MFYLKGNHNIIIEFNGQKPNETLSQVVFEGKSGAF", "aa_hexdigest": "714b5cc7067f23650074264dc18cf3d0", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3958.4535000000005, "isoelectric_point": 6.520194053649903 }, "id": "AHCKFKLKGB_566", "locus": "GCA013367975_00550" }, { "type": "cds", "sequence": "contig_1", "start": 313857, "stop": 314267, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22421.1" ], "nt": "ATGACTCAACACAATGAAAATAAAACCAAAAGAATTGCCCAAAAAAAATTAGCCTTTGAATTGGTTAAAGACATTCATGGAGTTGATATTGCAAACAAATGTATTAGAATAAGTGAAATTTTATTCAACAAAAGTAACGAAGAATTAAACATAGAAGATATTTCGATGCTTTTAGGTTATTTACCATGTATTGAAGTTAAAGAAGGCGAAAAATTTATTGAAATTTTAAAAAATAATCAAATTGTGTCTTCGAATAGAGAAGCAAGAGAATTTATTTCAAAAAATACTTTTTTAATTGATAACAATATAATAAACGATGAAAATTATTCAATTACATTTAATAAATTTAATGGTCAATATGCGCTATTAAAAAAAGGTAAAAAAGACTTTTACATACTTAAAAGAATTTAA", "aa": "MTQHNENKTKRIAQKKLAFELVKDIHGVDIANKCIRISEILFNKSNEELNIEDISMLLGYLPCIEVKEGEKFIEILKNNQIVSSNREAREFISKNTFLIDNNIINDENYSITFNKFNGQYALLKKGKKDFYILKRI", "aa_hexdigest": "6e6885b7adda3ce9c29f82cb536d06e9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 81, "aa_cov": 0.5808823529411765, "hmm_cov": 1.0, "evalue": 1.8534061801372557e-14, "score": 48.72767639160156, "start": 54, "stop": 132 } ], "seq_stats": { "molecular_weight": 15914.204799999985, "isoelectric_point": 8.798983573913574 }, "id": "AHCKFKLKGB_567", "locus": "GCA013367975_00551" }, { "type": "cds", "sequence": "contig_1", "start": 314727, "stop": 315092, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEF080B" ], "nt": "ATGCAAAAAAATATATTAGATGATTTGAAATCAAGGGGTGTTTTAAATAATATATCTAGTGAAGAAAAATTTAATAATATATCACTAGGAACAGGTGTTTATACAGGCTTTGATCCAACTGCTAAAAGTTTACACCTTGGAAATTATCTACAAATATTAAGTTTGTTACGTTTTAAAAAAGCAGGATACAAGCCAATTGCAATATTGGGTGGAATAACCGGAATGATTGGTGATCCTAGTTTTAGAAATTCTGAAAGAAAATTTCTAGATCAAGAAACATTACTACAAAATAAAAATGCAATTAAAAATCAATTACAAAAATTTGGTTTAGAAGTAATTGATAATTTAGATTTTTATAAACATTGA", "aa": "MQKNILDDLKSRGVLNNISSEEKFNNISLGTGVYTGFDPTAKSLHLGNYLQILSLLRFKKAGYKPIAILGGITGMIGDPSFRNSERKFLDQETLLQNKNAIKNQLQKFGLEVIDNLDFYKH", "aa_hexdigest": "4fd057b6db23b16aef4ac9231c5c4af3", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002FEF080B", "query_cov": 0.9752066115702479, "subject_cov": 0.9752066115702479, "identity": 0.6609999999999999, "score": 157.0, "evalue": 2.11e-47, "valid": false, "product": "tRNA synthetases class I (W and Y)", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_UPI002FEF080B", "kegg_orthology_id": "K01866" }, "pscc": { "uniref50_id": "UniRef50_UPI002FEF080B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEF080B" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13633.498899999982, "isoelectric_point": 9.362501716613771 }, "id": "AHCKFKLKGB_568", "locus": "GCA013367975_00552" }, { "type": "cds", "sequence": "contig_1", "start": 315426, "stop": 316097, "strand": "+", "frame": 3, "gene": "degV", "product": "DegV family protein", "db_xrefs": [ "COG:COG1307", "COG:I", "SO:0001217", "UniRef:UniRef50_A0A077L663", "UniRef:UniRef90_D1J851" ], "nt": "ATGCAAGTTATAGAAAAATATTTTAATGACTACGATAAAATTTTAGTTTATCCAATTTCAAATGCGCTTTCTTCGCAATGTGCTTCACTAACAGCACAATTTGCTTCAAATCCAAAAGTTCATGTAGTACAATCAAATAAAATTTCTTATCTGATTGTTAGAGACTTATTAATGTTTGAAGAATATATTAAAGAAGGAAAAAGCTTTGATGAAGCAATAGAACACTTTAAAAATAATAATGAAAGATTACTTCTAATTCCAGAGTTTAACGATGCCTTGCTAAAAGGCGGACGTTTATCAAAAGCAGCAGCCGCAATAGCAAAATTATTAAAAATAGTTCCTATTATTAAATTTGATAATGGAATTTTAGAAAAAGATGGTATTGGTAGAATCTTTAAAAAAACTCTAGAAAAAAATTTAAAAGAATTATATGAAAATACAGATAAAGATAATGAAAACAATTATCTATTAGTTGTAAATGCAAATAACGATAAAATAGATGAATTAATTCAGTCATTTAAACAAATAACTAATAATTTTCCAAGGATTTATACAATTCAATTAAGCTGCGACATTGTAGTTCATGCTGGTATTGGCGCAATCTGTTTAACATTTGCCGAAGTTAGTCCTAAAATAAAAGATAAATTCTTTTTATATTTTAAAAAACAATAA", "aa": "MQVIEKYFNDYDKILVYPISNALSSQCASLTAQFASNPKVHVVQSNKISYLIVRDLLMFEEYIKEGKSFDEAIEHFKNNNERLLLIPEFNDALLKGGRLSKAAAAIAKLLKIVPIIKFDNGILEKDGIGRIFKKTLEKNLKELYENTDKDNENNYLLVVNANNDKIDELIQSFKQITNNFPRIYTIQLSCDIVVHAGIGAICLTFAEVSPKIKDKFFLYFKKQ", "aa_hexdigest": "a967e341331bcf05b2aa6ffc9e653537", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "degV" ], "truncated": "5-prime", "pseudogene": { "start": 315216, "stop": 316097, "observations": { "mutation": [ 315282, 315369 ], "stop": [ 315282, 315369 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J851", "query_cov": 1.0, "subject_cov": 0.7610921501706485, "identity": 0.991, "score": 429.0, "evalue": 9.31e-151, "gene_start": 1, "gene_end": 223, "reference_start": 71, "reference_end": 293, "reference_sequence": "MKIKIITDSSAGMSQEQANSFGWDLIPIQIEVGNDIYKSGIDFDTDKFEELWRNDKKIDAKTFATPPGIAMQVIEKYFNDYDKILVYPISNALSSQCASLTAQFASNPKVHVVQSNKISYLIVRDLLMFEEYIKEGKSFDEAIEHFKNNNERLLLIPEFNDALLKGGRLSKAAAAIAKLLKIVPIIKFDNGILEKDGIGRIFKKTLEKNLKELYENTNKDNENNYLLVVNANNDKIDELIQSFKQITNNFPRIYTIQLSCDIVVHAGIGAICLTFAEVSPKIKDKFFLYLKKQ" }, "paralog": false, "identity": 0.9829351535836177, "score": 564.0, "evalue": 2.24e-206, "description": "Internal stop codon at 315282, 315369. Nonsense mutation around 315282, 315369.", "psc": { "uniref90_id": "UniRef90_D1J851", "gene": "DegV", "product": "DegV family protein", "uniref50_id": "UniRef50_A0A077L663", "cog_id": "COG1307", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J851", "UniRef:UniRef50_A0A077L663", "COG:COG1307", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L663" ], "product": "DegV family protein" } }, "id": "AHCKFKLKGB_569", "locus": "GCA013367975_00553" }, { "type": "cds", "sequence": "contig_1", "start": 316138, "stop": 316401, "strand": "-", "frame": 1, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "COG:COG0211", "COG:J", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "RefSeq:WP_175933476.1", "SO:0001217", "UniParc:UPI00158D9691", "UniRef:UniRef100_UPI00158D9691", "UniRef:UniRef50_O21275", "UniRef:UniRef90_D1J852" ], "nt": "ATGGCACATACGAAATCAGGCGGAACAACCACCAATAGCCGTGATTCAGCGGGAAGAAGATTAGGCGTAAAAGCAACCGACGGCCAATTTATTTTAGCAGGAAGCATTATTTATAGACAAAGAGGAACAAAAATTTTCCCAGGCCAAAATGTTGGTAGAGGAAAAGATGACACCTTATTTGCTTTAATCGATGGTATTGTAAAATTCGAAAGCAGAATTAACAGAAAATTTGCATCAGTTTACCCAATTGAAAGCAAGAAATAA", "aa": "MAHTKSGGTTTNSRDSAGRRLGVKATDGQFILAGSIIYRQRGTKIFPGQNVGRGKDDTLFALIDGIVKFESRINRKFASVYPIESKK", "aa_hexdigest": "93faf0c8e07f971978fe79efe7de3647", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158D9691", "ncbi_nrp_id": "WP_175933476.1", "uniref100_id": "UniRef100_UPI00158D9691", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D9691", "RefSeq:WP_175933476.1", "UniRef:UniRef100_UPI00158D9691" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D9691", "uniref90_id": "UniRef90_D1J852" }, "psc": { "uniref90_id": "UniRef90_D1J852", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_O21275", "cog_id": "COG0211", "cog_category": "J", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "genes": [ "rpmA" ], "id": "AHCKFKLKGB_570", "locus": "GCA013367975_00554" }, { "type": "cds", "sequence": "contig_1", "start": 316404, "stop": 316706, "strand": "-", "frame": 2, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855541.1", "SO:0001217", "UniParc:UPI0001BC223A", "UniRef:UniRef100_D1J853", "UniRef:UniRef50_Q6F123", "UniRef:UniRef90_D1J853" ], "nt": "ATGTTTGCTATTATTGAAACCGGCGGAAAACAATTACTTGTTAAAAGCGGTGATGTAATTTACATCGAAAAAATTGATGGCAATGAAGGTGACAAAGTTTCATTTGATAAAGTTTTAGTTATTGATGAACAAGTTGGCTCACCTTATCTACCAAAAGCTTCTGTTTTAGGAACCATTGAAAAACAAGGAAAAGCAAAAAAAATAGTTGTTTATAGACATAATGCTAAATCTACTCATAAACGTAAATTAGGACATCGTCAACCATTTACCAGAGTTAAAATTACAGAAATTAAGGAAAAATAG", "aa": "MFAIIETGGKQLLVKSGDVIYIEKIDGNEGDKVSFDKVLVIDEQVGSPYLPKASVLGTIEKQGKAKKIVVYRHNAKSTHKRKLGHRQPFTRVKITEIKEK", "aa_hexdigest": "2e1b8b13d765a237bc4b0b6090d9d3bf", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC223A", "ncbi_nrp_id": "WP_012855541.1", "uniref100_id": "UniRef100_D1J853", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC223A", "RefSeq:WP_012855541.1", "UniRef:UniRef100_D1J853" ] }, "ips": { "uniref100_id": "UniRef100_D1J853", "uniref90_id": "UniRef90_D1J853" }, "psc": { "uniref90_id": "UniRef90_D1J853", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_Q6F123", "cog_id": "COG0261", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6F123", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6F123" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "AHCKFKLKGB_571", "locus": "GCA013367975_00555" }, { "type": "cds", "sequence": "contig_1", "start": 317322, "stop": 317951, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAATTCAAAGAATTTATCGTATTCAAGGTATTGCAATTAGCGATAAGTATATTGAAATTATAATTCGTCAAATGATGTCTAAATTAGTAATTTCTGAACCCGGAGATTCTAAATTCTTCGCTGGTGCTATTGTAGATATTTTTGACTATCAAGAAGAAAATGCTATTCTACTTTCAAAAGGTTTGAAGCCAGCATATGGTAAAGTTGTTATTAAAGGTGCAAAACAAGTTCCATTGTTATCTGATTCATTCCTTGCCGCTGCTTCTTATCAAGAAACAAGCAAGATATTAGTTCACGCTGCTATTTCATCAAGAATTGACAATTTATCTGGCTTAAAAGAAAATATTATTCTTGGTAAAAAGATTCCATCAGGAACAGCATTATTCCCATTTGAAGAACATTCAAAATATGATATAAAACCATCAATTAATTACTTTACAACAACTCCTATTGACGGCACAGAAATTTCTGAACCTATTGATATTGATTCAGTTGTTAATACAGCGCAAGAATATGACAATGATAAGCAATCAGTTGAAGAAGATTATTCTCCAAACGACGAAGAACTTGTTGCTGAAGAAGAAACATTTGATGATGAAGAAAATCAAAACGATTTTGAATAA", "aa": "MKEIQRIYRIQGIAISDKYIEIIIRQMMSKLVISEPGDSKFFAGAIVDIFDYQEENAILLSKGLKPAYGKVVIKGAKQVPLLSDSFLAAASYQETSKILVHAAISSRIDNLSGLKENIILGKKIPSGTALFPFEEHSKYDIKPSINYFTTTPIDGTEISEPIDIDSVVNTAQEYDNDKQSVEEDYSPNDEELVAEEETFDDEENQNDFE", "aa_hexdigest": "fbd290a4b427df44851d560d6d23fcd8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23438.981199999995, "isoelectric_point": 4.424085807800293 }, "id": "AHCKFKLKGB_572", "locus": "GCA013367975_00556" }, { "type": "cds", "sequence": "contig_1", "start": 318219, "stop": 318980, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "ATGATTAATTCAGGTGCTCGTGGTAACATTTCTAACTATGTTCAATTAGCAGGTATGCGTGGATTGATGGCTAACAATACTAAAACTACTAAGGCCGATGCTAAAAACGACCGTGTTGTTAGATCAACAGTTGAAGTTCCTGTTAAGTCTTCATTTATTGAAGGACTTACTGCATTTGAATTCTATTCATCAACTCACGGTGCTAGAAAAGGTTTAACCGATACTGCCTTGAACACTGCAAAATCTGGTTATTTAACAAGACGTTTAGTTGATGTTGCTCAAAATATTGTTGTTAGAGAAGAAAACTGTGGTACTGACTATGGTTATAAAGCAAGAAACATAGTTGATACAAAAACTAAACAAATTATTGTTCCTTTGAAAGAAAGAATTGTTGGAAGATTTGCAAACAAACCTGTTGTTACAAGTGCGGGTATAGAAATTTGTTCTAGAAATGAATTAATTACTGAAAAAATTGCTCAACGAATAATAGCAAGTGGTATTGAAGAAGTCGAAATTAGATCAATCTTAGGCTGCAATACACGTAACGGTGTATGTAAGATGTGTTTTGGTAAAGACCTTGCAACAAATAGAATTGTTAATATCGGTGAAGCAGTAGGTATTATTGCAGCTCAATCTATTGGGGAACCAGGAACTCAATTAACTATGCGTACATTCCACACTGGTGGTGTTGCCGGGGTTGAAGATATTACTGGTGGTTTCACTAGATTGATCGAATTAATTGATGCCCACGAACAACCATGA", "aa": "MINSGARGNISNYVQLAGMRGLMANNTKTTKADAKNDRVVRSTVEVPVKSSFIEGLTAFEFYSSTHGARKGLTDTALNTAKSGYLTRRLVDVAQNIVVREENCGTDYGYKARNIVDTKTKQIIVPLKERIVGRFANKPVVTSAGIEICSRNELITEKIAQRIIASGIEEVEIRSILGCNTRNGVCKMCFGKDLATNRIVNIGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGVEDITGGFTRLIELIDAHEQP", "aa_hexdigest": "0d69966ffc331c42d09c365b161e2ab6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 56, "aa_cov": 0.20948616600790515, "hmm_cov": 0.9821428571428571, "evalue": 5.004684772293286e-12, "score": 41.05525588989258, "start": 1, "stop": 53 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 195, "aa_cov": 0.7707509881422925, "hmm_cov": 0.6, "evalue": 5.084199725120017e-47, "score": 155.84100341796875, "start": 55, "stop": 249 } ], "seq_stats": { "molecular_weight": 27363.022100000013, "isoelectric_point": 9.110559272766114 }, "id": "AHCKFKLKGB_573", "locus": "GCA013367975_00557" }, { "type": "cds", "sequence": "contig_1", "start": 319098, "stop": 319253, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAGTATTAAACAATTAGGTTTTGACTATTCAACATATTCTGGAATTTCTATTTCGTTTAGTGATATTCTAGAAACTAATAAAAAGCAAGAATATATTGCTCAAGGTGATGAATATATTGCTAAATTAAAACACTATTATGAACAAGGTTGA", "aa": "MDSIKQLGFDYSTYSGISISFSDILETNKKQEYIAQGDEYIAKLKHYYEQG", "aa_hexdigest": "fd1d13a9a161c1bc127e024eb4652b75", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5917.4749, "isoelectric_point": 4.749659538269043 }, "id": "AHCKFKLKGB_574", "locus": "GCA013367975_00558" }, { "type": "cds", "sequence": "contig_1", "start": 319284, "stop": 320096, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04983.23" ], "nt": "ATGGCTGTTCACGTTCCTGTTTCTGAACAAGCATTGCTTGAAAGTCGTGAATTAATGTTGGCTAATAAAAACATTCTAGGACCTAAAGATGGTGAACCTATTATTAACCCTTCTCAAGATATGGTTTTAGGTATTTATTATCTAACTATTGAAGAACCAGGAGCTAAAGGTGAAGGACGTGTTTTTGATAACTATGAACACATGCTTAGAGCATTAGAAAATAAAGTAGTTACATTGCACACTAGAATAGCATTACCTGCTTCAGAAGTTAAAACTCCAAAACTTATTTTCCCATTTGATGGTCCTCAATATATAATTTCAACAGTAGGTAAATTTATTTTTAATAATATATTCCCTAAAACATTTCCATTTATTTACGACAATAAAGTTGCTAAGGCAAATTCATTGGAAGATTATAATCAAACATTCAATGCAAAATATGTCGTTCAAGCAGGTACTAATATTCCTGAATATATCAAAAATATGCCTATTGAAGAAGCATTTAACAAAAAGAATATTTCTAAAATAATACGTTATGTTTTTGACAAATATGTTGCTCAAATTTCAATGTCTGATATTGCAGAAGTTATTAATAATTTAACTGAATCAAGTGATGCTAATGATTCAATAATTCAATTCTTGCATGTTAAAAATTATGAAAATAGAAAAATTGATTATGAACATGCAATGTTGCTAGCTCAATTTACAAGTCAAGAAAAGCAAAAGATTGAAATGTTAAATCCTGCTAGGGCAAATGGACAAGAAGTTCCACTTAGTCCAGCTGAAAAAGCAGAAATTCTAGACACTGTATGA", "aa": "MAVHVPVSEQALLESRELMLANKNILGPKDGEPIINPSQDMVLGIYYLTIEEPGAKGEGRVFDNYEHMLRALENKVVTLHTRIALPASEVKTPKLIFPFDGPQYIISTVGKFIFNNIFPKTFPFIYDNKVAKANSLEDYNQTFNAKYVVQAGTNIPEYIKNMPIEEAFNKKNISKIIRYVFDKYVAQISMSDIAEVINNLTESSDANDSIIQFLHVKNYENRKIDYEHAMLLAQFTSQEKQKIEMLNPARANGQEVPLSPAEKAEILDTV", "aa_hexdigest": "e47c54f02340f394fb31d6537a96b9d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04983.23", "name": "RNA_pol_Rpb1_3", "length": 182, "aa_cov": 0.6518518518518519, "hmm_cov": 0.8131868131868132, "evalue": 2.2305566102126227e-26, "score": 87.98047637939453, "start": 24, "stop": 199 } ], "seq_stats": { "molecular_weight": 30617.709800000026, "isoelectric_point": 5.372728538513184 }, "id": "AHCKFKLKGB_575", "locus": "GCA013367975_00559" }, { "type": "cds", "sequence": "contig_1", "start": 320352, "stop": 320627, "strand": "-", "frame": 2, "gene": null, "product": "DNA-directed RNA polymerase subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "nt": "TTGGTTATTCAAAACGAACTAAGAATGATTCAAGAAGCAGTAGATGCTTTAATTGATAACCAAAGAAGAACACCTAACCCTGTTTTATCAAAAGACAATCGTCCATTTAAATCAATTTCAGATGCATTAACTGGTAAAAAAGGTCGTTTTAGACAAAATTTACTAGGAAAACGTGTTGACTATTCTGGACGTTCAGTTATTGCTGTAGGGCCTTCATTAAAAATGCACCAATGTGGTATACCAAGAGAAATGGCTGCTAAATTATTTGAACCATGA", "aa": "MVIQNELRMIQEAVDALIDNQRRTPNPVLSKDNRPFKSISDALTGKKGRFRQNLLGKRVDYSGRSVIAVGPSLKMHQCGIPREMAAKLFEP", "aa_hexdigest": "4958223e7fd21991b5516508c96ea886", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002381C6D8", "query_cov": 0.9340659340659341, "subject_cov": 1.0, "identity": 0.624, "score": 112.0, "evalue": 1.02e-30, "valid": false, "uniref50_id": "UniRef50_A0A3B9S0R9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B9S0R9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "product": "DNA-directed RNA polymerase subunit" }, "genes": [], "id": "AHCKFKLKGB_576", "locus": "GCA013367975_00560" }, { "type": "cds", "sequence": "contig_1", "start": 320649, "stop": 321335, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "TTGCTTGGTTTAAAATCTTCAAATGGTTCTGACGTGGTTAAACGTGAAGAACTAGAAAATTTAATTTATTACAAAAATCATATTGTTCTAGAAGATGGTGGCTTAAAATCATTACCTAAAAATCAAATTATTGAAATCAATGAAGCAGCAATCATTTACCGCAATGCTCTAGAAGAATTGCTTGAAAGATTTCAAGAAGGTACAGAAGAATATGATGATATTAAAGAAACCCTAGATTCTTTAATTGAAAAAGCAACATCAAAAATCGGTCAAGAATATGGAATTGATTTTTATGAATTAAACGAAGTAATTGAACATTATTCAGATGCTAAAATAGGTACTGGTGCTTTAGCTATTGAATATTTATTAAAAAACATAAATTTAGCTGAAGAAAAGCAAAAAGTAACTGATGAAATTAATCGTTTAAATGCAGAAGAAATTAATAATCCTGAACGTTTTGCCACAACTGCAAAACAAACAAGAGAAAAACTTTATAAACGTTTGCAAGTAATTAATGCATTTATTGAATCTAAACAAGATCCAACTAGCATGTTAATTTATAATTTGCCTGTTATTCCAGCAGATTTAAGACCTCTAATTCAATTAGATGGTGGTAGACACTCAACTAGCGATATTAATGAATTGTATCGTAGAGTAATTATTAGAAACAATAGATTGCAACAATGA", "aa": "MLGLKSSNGSDVVKREELENLIYYKNHIVLEDGGLKSLPKNQIIEINEAAIIYRNALEELLERFQEGTEEYDDIKETLDSLIEKATSKIGQEYGIDFYELNEVIEHYSDAKIGTGALAIEYLLKNINLAEEKQKVTDEINRLNAEEINNPERFATTAKQTREKLYKRLQVINAFIESKQDPTSMLIYNLPVIPADLRPLIQLDGGRHSTSDINELYRRVIIRNNRLQQ", "aa_hexdigest": "c57cfefce064fd45e8f993c30529a07d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 222, "aa_cov": 0.9342105263157895, "hmm_cov": 0.7207207207207207, "evalue": 1.075500154154568e-22, "score": 76.0958480834961, "start": 16, "stop": 228 } ], "seq_stats": { "molecular_weight": 26268.37680000003, "isoelectric_point": 4.939615440368651 }, "id": "AHCKFKLKGB_577", "locus": "GCA013367975_00561" }, { "type": "cds", "sequence": "contig_1", "start": 321369, "stop": 321728, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "ATGAAAAAAATTTCAAAATACGAATCATTAGATGAAGAAAAAATTTCAAAAATTTCATTAGCATTAGCAACTCCTCAAGATGTTGAAACTTGGTCTCATGGTGAAGTTACTAAGCCAGAAACTATTAACTATAAATCATATAAACCAGAACGTGGTGGATTATTTGATGAAATAATTTTTGGACCAATGATTGATTATCGTTGCCCTGTATGTGGTTTCAAATACAAAAAAGTTAATGAAGGAAGCTATTGTACAAGAACTGAATTATGTAAACAAGAACATGTTCAAATATTGCCTAAAATAGCACGTAGAACCCATATGGGACATATAAAACTAAATAGTCCAGTTGTTCACTTTTGA", "aa": "MKKISKYESLDEEKISKISLALATPQDVETWSHGEVTKPETINYKSYKPERGGLFDEIIFGPMIDYRCPVCGFKYKKVNEGSYCTRTELCKQEHVQILPKIARRTHMGHIKLNSPVVHF", "aa_hexdigest": "7ab28ebbdd5fde8231c32dc012a94f7b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 109, "aa_cov": 0.8991596638655462, "hmm_cov": 0.7522935779816514, "evalue": 4.773809678963655e-24, "score": 80.53942108154297, "start": 13, "stop": 119 } ], "seq_stats": { "molecular_weight": 13760.796499999986, "isoelectric_point": 8.857262992858885 }, "id": "AHCKFKLKGB_578", "locus": "GCA013367975_00562" }, { "type": "cds", "sequence": "contig_1", "start": 321730, "stop": 322914, "strand": "-", "frame": 1, "gene": null, "product": "DNA-directed RNA polymerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352BHB6" ], "nt": "TTGGTTAAAGATGATGTTTATACATCAATTCACATTGAAGAACAATCAATTCAATTTAGACATTCAAAGGCTGGCGAAGATATTTTAACTGCAGATATTCCTAACGTTTCAAACTATTCTAAACGTTTCTTAGGTGAAAATGGAATTGTTAGAGTTGGTTCAGAAGTAACAGCAGGTGATATTTTAGTCGGAAGAACTTCTCCAAAAGGTGAAGAAAACCCAACTCCTGAAGAAAAATTAATGGCTGCAATTTTTGGTCAAAAAACTGCCTCAAGAAAAGATACTTCTCTAAAAGTAAAACATGGCCACAATGGAACTGTTGTTGCTGTTGATATTCTAAGTAGAGAATTTGGCGACCAATTAGAAGATGGCATTGAAAAAATAGTTAAGGTATCAATTGCTCAAAAACGTAAAATTCAAGTCGGCGACAAGATGGCTGGTCGTCACGGAAATAAAGGTGTTGTTTCAATAGTATTGCCTGTTGAAGAAATGCCTTATTTAGCAGATGGAACACCATTAGATATTGTTTTAAACCCTCAAGGTGTCCCTTCACGTATGAACATTGGTCAAGTTCTAGAATTGCACTTAGGACTTGCTGCTAAAAAATTAAATACAAAATTTGTTACACCAATATTTGATGGAATTTCACACAACCAAATTCGTGATATTCTTGAAGAAGCAAATATTGATGTAACAGGAAAATCAATAGTATACGATGGGGCCACTGGAGAACCATTTGATCACCCAATTTCTGTAGGGATTATGTACTATTTGAAGTTGTATCACATGGTTGATGACAAAATGCACGCACGTTCAGTAGGACCTTATTCATTGATTACCCAACAACCACTTGGTGGAAAGAGTCAAAATGGTGGTCAAAGATTTGGTGAAATGGAAACATGGGCTATTGAATCATATGGAGCTTCAAATGTTCTACAAGAATTATTAACATATAAATCTGATGATATTGTTGGACGTAATAAACTATATAACTCTCTTGCTAGAGATGCAAGATTGCCTAAACCAGGAACACCTGAATCATTTAATGTTTTAGCATATGAATTACGTGGTTTAGGAATTAAATTAGAAGTACATCAAAAATCTGATAATGTCGTAGATGATTCAGAAGAAGATCAAAGAGTAGACACAAAATATTTCCAAGAATTTGAAGGGAGTTTAGAATAA", "aa": "MVKDDVYTSIHIEEQSIQFRHSKAGEDILTADIPNVSNYSKRFLGENGIVRVGSEVTAGDILVGRTSPKGEENPTPEEKLMAAIFGQKTASRKDTSLKVKHGHNGTVVAVDILSREFGDQLEDGIEKIVKVSIAQKRKIQVGDKMAGRHGNKGVVSIVLPVEEMPYLADGTPLDIVLNPQGVPSRMNIGQVLELHLGLAAKKLNTKFVTPIFDGISHNQIRDILEEANIDVTGKSIVYDGATGEPFDHPISVGIMYYLKLYHMVDDKMHARSVGPYSLITQQPLGGKSQNGGQRFGEMETWAIESYGASNVLQELLTYKSDDIVGRNKLYNSLARDARLPKPGTPESFNVLAYELRGLGIKLEVHQKSDNVVDDSEEDQRVDTKYFQEFEGSLE", "aa_hexdigest": "64194ddd54b985f768b118196d0dc5de", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030B82D9C", "query_cov": 0.9873096446700508, "subject_cov": 0.8644444444444445, "identity": 0.715, "score": 555.0, "evalue": 5.4e-195, "valid": false, "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_A0A352BHB6", "kegg_orthology_id": "K03043" }, "pscc": { "uniref50_id": "UniRef50_A0A352BHB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352BHB6" ], "product": "DNA-directed RNA polymerase" }, "genes": [], "id": "AHCKFKLKGB_579", "locus": "GCA013367975_00563" }, { "type": "cds", "sequence": "contig_1", "start": 322957, "stop": 324162, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04565.21", "PFAM:PF10385.14", "PFAM:PF00562.33" ], "nt": "ATGTTGCATGACCAAAAAGTTTTGGTTTTAGGTAATGATCCTACATCAACAGAAAATTTCTTGTTGATTCCTGATTTAATTGCAACGATTTCGTATTATTTTAATTTACTTTCAAAGGTTGGGGTTGATGATGATCCTGACTCATTAGTTAATAAAAGAATTGTAACAATAGGCGAATTATTACAAAATCAATTTAGAATAGGGCTATTAAAATTAGAAAAAAATACAAGAGAAAGAATCTCGGCAAAAGATATTGATAAGATTACTGCTAAGAACGTTACAAACAATAAACCAATATTTAATCAATTTAAATCGTTCTTTAACACATCAAAACTTTCTCAATTTATGGATCAATGTAATCCTCTTGCTGAAATATCAAACAAGAGAAGAATAACCTCCCTAGGACCAAGAGGGTTGAACCGTGATACTGCACAATTTGAAGTTCGTGACGTTCACCCTACACATTATGGAAGAATTTGTCCTATTGAAACACCCGAAGGACCAAACATTGGTTTAATTTTAAATTTAGCATCATTTGCTAAAATTGATAAATATGGTTTTATTCAATCACCATATTTCAGAGTAAATAAAAAAGTTGTAGATTTTTCAGAACCAGTTTACATTACCGCAATTGAAGAAACAGGATATACATTTGCACAATCAACTGTTCACATTGAAGGTAATGTAATTGTTGATGAAAAAGTAATGGTAAGACGTGACAATGAATATTTAGAAGTAAATGCTGAAGATGTAGATTTTATTGGTGTCTCAAACCGTCAAATGACCTCAATAGCTGCTTCGGCTATACCATTTTTGGAAAACGATGATGCTAACCGTGCATTGATGGGTTCAAACATGCAACGTCAAGCTGTTCCAGTGTTATTCCCTGAAGCACCATTAGTTGCTACAGGAGTTGAAGCTGACATTGCTAAATATTCATCAACCAATATTAGAGCAACCAGATCAGGGGTTGTTAACTATGTAGATGCTAACGTTATTAAAGTAACTCCTGAAAATAGTTCAAAAAGTGATGTTTATCATTTAAGAACATTTGAAAGAAGTAACCAAGGTACATTAATTCACCAAGTACCTTTGGTTAAAGTTGGCCAACAAATTAATGCAGGAGATTTATTAGTTGATGGTCCTTCAATGAAAGATGGAGAATTAGCATTAGGAAAAAATGTTCTAGTAGGTTTTACTACTTGA", "aa": "MLHDQKVLVLGNDPTSTENFLLIPDLIATISYYFNLLSKVGVDDDPDSLVNKRIVTIGELLQNQFRIGLLKLEKNTRERISAKDIDKITAKNVTNNKPIFNQFKSFFNTSKLSQFMDQCNPLAEISNKRRITSLGPRGLNRDTAQFEVRDVHPTHYGRICPIETPEGPNIGLILNLASFAKIDKYGFIQSPYFRVNKKVVDFSEPVYITAIEETGYTFAQSTVHIEGNVIVDEKVMVRRDNEYLEVNAEDVDFIGVSNRQMTSIAASAIPFLENDDANRALMGSNMQRQAVPVLFPEAPLVATGVEADIAKYSSTNIRATRSGVVNYVDANVIKVTPENSSKSDVYHLRTFERSNQGTLIHQVPLVKVGQQINAGDLLVDGPSMKDGELALGKNVLVGFTT", "aa_hexdigest": "a5684565194a5de4b4ea6da7d1130d75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04565.21", "name": "RNA_pol_Rpb2_3", "length": 69, "aa_cov": 0.17206982543640897, "hmm_cov": 0.9855072463768116, "evalue": 6.479462898556764e-30, "score": 98.50003814697266, "start": 114, "stop": 182 }, { "id": "PF10385.14", "name": "RNA_pol_Rpb2_45", "length": 67, "aa_cov": 0.16458852867830423, "hmm_cov": 0.9850746268656716, "evalue": 1.956817584083987e-15, "score": 51.9917106628418, "start": 192, "stop": 257 }, { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 86, "aa_cov": 0.20448877805486285, "hmm_cov": 0.8837209302325582, "evalue": 1.682379483910964e-10, "score": 35.854862213134766, "start": 319, "stop": 400 } ], "seq_stats": { "molecular_weight": 44613.200400000074, "isoelectric_point": 5.792484474182129 }, "id": "AHCKFKLKGB_580", "locus": "GCA013367975_00564" }, { "type": "cds", "sequence": "contig_1", "start": 324334, "stop": 324660, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04561.19" ], "nt": "ATGTTTGGCGAAAACATTCCAGAATTAGAAGAAACATTTAAACGTGATAAAATCACTGGAATTAATGCAGAAGAAGTTACAAGGGAAGCACAAGAAGCAATTTATAGAATCATTAGAAAAGGCGATCGTATGACAGCTGATTCTGCTAGAAATTTAATTCCTACAACTTTGTTTAATGAAAAAAGATATAGTTTAACTGAAACAGGACGTTTCACTTTAAATAGAAAATTAAACATTATTGAAAGAATTGCCAATTCTTATTTAGCAGAAGATATTTTTTCTAGTGAAGGTGAACTTCTTTATGAAAAAGGAATGTTCATTACTTGA", "aa": "MFGENIPELEETFKRDKITGINAEEVTREAQEAIYRIIRKGDRMTADSARNLIPTTLFNEKRYSLTETGRFTLNRKLNIIERIANSYLAEDIFSSEGELLYEKGMFIT", "aa_hexdigest": "381928d4fce804669575b1b026c59d28", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04561.19", "name": "RNA_pol_Rpb2_2", "length": 71, "aa_cov": 0.6296296296296297, "hmm_cov": 0.9154929577464789, "evalue": 2.538210525060132e-06, "score": 22.57516098022461, "start": 27, "stop": 94 } ], "seq_stats": { "molecular_weight": 12558.086299999986, "isoelectric_point": 5.114622688293457 }, "id": "AHCKFKLKGB_581", "locus": "GCA013367975_00565" }, { "type": "cds", "sequence": "contig_1", "start": 324790, "stop": 325329, "strand": "-", "frame": 1, "gene": null, "product": "RNA polymerase beta subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFDE54", "UniRef:UniRef90_UPI002FDFDE54" ], "nt": "ATGGTGAATAAGGATAAATACGAAATTAGAAAATTTGGACCTATTACCCTAAGACGTGACTATTCAATTAGTCAAAAGAAATTCGAAACTCCTGACTTTCTTGAAATGCAACGTGAATCAGTTGAAAAATTTCTAACAGTTGGAATTGAAGAAGAATTAAGAAATATCTATCCAATTGAAGCACATGGAAAAGTTAGAATTGAATACATTCATAATTCAGCACATTTTGAATATCCTAAAAAAACTGAATATGAAAGCATTAAAGAAGCTAAACAAAAAGGTTCATCATATCAAGGAAAGTTAAAAGCACACCTAAGACAAATCAATATCGAAACAGGTGAAGTTGAAGATTCAGAAGTAGTATTTGCTGAAATTCCTATAATGACTTATGGTGGATCATTTATTATCAATGGTTCAGAAAAAGTTATTGTTTCGCAATTAATTAGATCTACCGGGGCTTATTTTGGAATTAATGTTAGAAATAAACAAGCCAACGACTTGTTTAATAAAGTAGAAATTATTCCTCAAATTGGTTCATGA", "aa": "MVNKDKYEIRKFGPITLRRDYSISQKKFETPDFLEMQRESVEKFLTVGIEEELRNIYPIEAHGKVRIEYIHNSAHFEYPKKTEYESIKEAKQKGSSYQGKLKAHLRQINIETGEVEDSEVVFAEIPIMTYGGSFIINGSEKVIVSQLIRSTGAYFGINVRNKQANDLFNKVEIIPQIGS", "aa_hexdigest": "73e8e121e79577765c555dbc7556d4bc", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 324487, "stop": 325320, "observations": { "mutation": [ 324792 ], "stop": [ 324792 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FDFDE54", "query_cov": 0.9832402234636871, "subject_cov": 0.5482866043613707, "identity": 0.8859999999999999, "score": 307.0, "evalue": 4.96e-103, "gene_start": 4, "gene_end": 179, "reference_start": 3, "reference_end": 178, "reference_sequence": "MVKAKYELRKFGPITERRDYSISQKKFATPDFLEMQRTSVENFIHQGIEEELRNIYPIESHGKVRIEYVWNSAHFDYPKKSEFESIKEAKQKGSSYQGKLKARLRQINLETGEVEESEVVFAEIPIMTYGGSFIINGSEKVIVSQLIRSTGAYFGLNVRNKQANDLFNKVEIIPQVGSWVEIYHKVTSANADSVKIHIDKNKSFLLTTFLKALGFSETGIRAMFGDIAELEETLKKDKIVGLSLEESTREAQEAIYRIIRKGDRMTVESARNLIPSTLFNEKRYNLTQIGRFTLNRKLNVIERIANSFLAESIFSDKGELL" }, "paralog": false, "identity": 0.851985559566787, "score": 465.0, "evalue": 1.04e-167, "description": "Internal stop codon at 324792. Nonsense mutation around 324792.", "psc": { "uniref90_id": "UniRef90_UPI002FDFDE54", "product": "RNA polymerase beta subunit", "uniref50_id": "UniRef50_UPI002FDFDE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FDFDE54", "UniRef:UniRef50_UPI002FDFDE54" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FDFDE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFDE54" ] } }, "id": "AHCKFKLKGB_582", "locus": "GCA013367975_00566" }, { "type": "cds", "sequence": "contig_1", "start": 325463, "stop": 325837, "strand": "-", "frame": 3, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_175933477.1", "SO:0001217", "UniParc:UPI00158C6AE2", "UniRef:UniRef100_UPI00158C6AE2", "UniRef:UniRef50_B3PM75", "UniRef:UniRef90_B3PM75" ], "nt": "ATGGCAAAATTAACAAAAGAAGAATTTGTTTCAGCATTAAAAGAAATGAACATTAAAGAAGTTATGGAATTAATTCAAGGATTAAAAGAAGAATTTGGAATTGATCCAACAGCAGTTGTAGCAGCAGCAGCTCCAGCAGCAGCAGCTGACGCAGTAGAAGAAAAGACTGTATTTAATGTTACTTTAAAATCAGATGGTGGAAACAAGCTAGCAGTTATTAAAGTTGTTAAAGACTTATTAGGTCTAGGATTAATGGATGCTAAAAAACTTGTTGAATCAGCACCTGTTGTAATTAAAGAAAATGCTAAAAAAGAAGAAGCTGAAGAACTAAAAGCTAAATTAACAGAAGCTAAAGCCGAAGTTACTTTAGACTAA", "aa": "MAKLTKEEFVSALKEMNIKEVMELIQGLKEEFGIDPTAVVAAAAPAAAADAVEEKTVFNVTLKSDGGNKLAVIKVVKDLLGLGLMDAKKLVESAPVVIKENAKKEEAEELKAKLTEAKAEVTLD", "aa_hexdigest": "1475b68516aed80ea0ef09b8aac3df85", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158C6AE2", "ncbi_nrp_id": "WP_175933477.1", "uniref100_id": "UniRef100_UPI00158C6AE2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C6AE2", "RefSeq:WP_175933477.1", "UniRef:UniRef100_UPI00158C6AE2" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C6AE2", "uniref90_id": "UniRef90_B3PM75" }, "psc": { "uniref90_id": "UniRef90_B3PM75", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_B3PM75", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM75", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM75" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "AHCKFKLKGB_583", "locus": "GCA013367975_00567" }, { "type": "cds", "sequence": "contig_1", "start": 325860, "stop": 326357, "strand": "-", "frame": 2, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02864", "RefSeq:WP_012855545.1", "SO:0001217", "UniParc:UPI0001BC223D", "UniRef:UniRef100_D1J857", "UniRef:UniRef50_A0A448ZYM3", "UniRef:UniRef90_D1J857" ], "nt": "ATGAGCGCATTAAAAGATGCCAAGGTTTTAGTTGTTGAAGAAATTTCAAACAATTTAAAAGAATCACAAGCATTATATGTAATTAATTATGCTACTTTGGATGTTGTTTCATTTCAAGAAATTAGAAAAGAATTAGCTCCAAATAATGCATTGCTAAAAGTTTACAAAAATAGACTAGTTAAACAAGCATTAAAAAATACAGAATATGCAAATATTAATGATAGTTTAGTTTTACAAAATGCTTATGCCTTTGTTAAAGGCGATTCATTAGCAGCATTAAAAACCTTAGTAGGCTTAAAGAAGAAATTCCCTGCATTAAGAATTGTAAGTGGAATTTATGAAAATAAAGTAGTTGATGAAAAAACATTAGACGAAATATCAAAACTTCCTTCATTTACAGAATCACTTATGATTCTTGGAAATTCATTATTGAGTCCATTGAAACAATTATCAATTGGTTTAAATGAATTAATAAAACAAGGAAAAATTTCAGAATAA", "aa": "MSALKDAKVLVVEEISNNLKESQALYVINYATLDVVSFQEIRKELAPNNALLKVYKNRLVKQALKNTEYANINDSLVLQNAYAFVKGDSLAALKTLVGLKKKFPALRIVSGIYENKVVDEKTLDEISKLPSFTESLMILGNSLLSPLKQLSIGLNELIKQGKISE", "aa_hexdigest": "4e952e6b06822b7a4ca63497eef65389", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC223D", "ncbi_nrp_id": "WP_012855545.1", "uniref100_id": "UniRef100_D1J857", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC223D", "RefSeq:WP_012855545.1", "UniRef:UniRef100_D1J857" ] }, "ips": { "uniref100_id": "UniRef100_D1J857", "uniref90_id": "UniRef90_D1J857" }, "psc": { "uniref90_id": "UniRef90_D1J857", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_A0A448ZYM3", "cog_id": "COG0244", "cog_category": "J", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYM3" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "AHCKFKLKGB_584", "locus": "GCA013367975_00568" }, { "type": "rRNA", "sequence": "contig_1", "start": 326578, "stop": 329472, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9897435897435898, "score": 2757.9, "evalue": 4.6e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TATTAAACAAGCAAGAGTTTTTGGTGGATGCCTTGGGTCTGGAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGGTTAGCTGGAAATAAGCTGTTATCCGGGGATTTCCGAATGGGGAAACCTAATTGAGCTAATCCTCAATTATCATATCGATGAATTCATAGTCGAATGAAGAGACACGCTGTGAATTGAAACATCTCAGTAGCAGCAGGAAGAGAAAATAAAGAATGATTCCCTAAGTAGTGGCGAGCGAACGGGGAAGAGCCCAAACCAACACATGTTGTTGGGGTTGTAGGACTGCGACATGGATTAATGAATTTGTACATAGCAGAATAAGTTGGAACGCTTAAACATAGAGGGTGAAATTCCCGTAAGCGAAATGTATAAATCTCCTAGCAGTATCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATTTGCCGGGACCACCCGGTAAGGCTAAATACTAACCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGGGAGTGAAATAGATTCTGAAACCAATTACTTACAGTTAGTCAGAGCCCGTTAATGGGTGATGGCGTACATCTTGCAGAATGGACCGGCGAGTTATGTCAACATGCGAGGTTAAGTAGAATAAAGCGAAGCCGTAGAGAAATCGAGTCTGAATAGGGCGATTTAGTATGTTGATATAGACCCGAAACCAGGTGATCTACCCATGAGCAGGTTGAAACTTAGGTAACACTAAGTGGAGGACCGAACCGCAGTACGCTAAAAAGTGCCCGGATGACTTGTGGGTAGGGGTGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAATGATGGGGGTAGAGCACTGAATATGGAATGGCGGCGCCTAGCCGTACTGACTATAATCAAACTCCGAATACTATCATGTATTACTATGCAGTCGGTACATCGGTGATAACGTCGATGCACGCGAGGGGAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGTGTTAAGTGAGAAAGGTTGTGAAGTTTCTTAAACAGCTAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAATAGCTCACTAGTCGAGAGACTTTGTGCCGATAATTCAACGGGACTAAAACACAATACCGAAGCTACGGGCAGAAATGCGTTAGGAGAGCGTTGTAAGGGCTTAGAAGCCAGACCGTGAGGACTGGTGGAGCGCTTACAAGTGAGAATGCCGGTATGAGTAACGATTCAGAGTGAGAATCTCTGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCGGGTCCTAAGACGAGGCCGAAAGGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTTAACATGTGATGGAGTGACGGGGAAGGATAATTTTACCACTAATTGGATTGTGGGGTAAGTAACAACTGGGTTATGTAGGCAAATCCGCATAACTTAACTGGGAGTTACGACGCATAGCAAAAGGGCAACTGAGTAGCGAATTAGATGATTTCATGCCTCTTAAAAAAGCTTCTAACATTAAGTGTTAGGAAACCCGTACCGAGAACGGACACACGTCCCCAAGATGAGTATTCTAAGGCGAGCGAGAAAACTATTGTCAAGGAACTCTGCAAATTCATCCCGTAAGTTCGCAAGAAGGGATGCCCACAATAAAATGTGGGCCGCAGTGAATAGTAAGGGGGAACTGTTTATCAAAAACACAGCTCTATGCTAAGTCGTAAGATGATGTATATGGGGTGACTCCTGCCCAGTGCCCGAAGGTTAAGCAGAGGTGTTAGCATTAGCGAAGCATTAATGTGAAGCCCGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGAGTAATTATCTCTTAACTGTCTCGACAATAGACTCGGTGAAATTATGGTTCCGGCGAAGACGCCGGAGACCCGCATCTAGACGAAAAGACCCCGTGGAGCTTTACTATAACTTCATATTGGAGTTTGATTTAACATGTGTAGGATAGGTGGGAGACGTTGATGCTTGAACGCTAGTTTAAGAGTAGTCGCCGTTGAAATACCACCCTTGTTACATTGAACTTCTAACTTGTTCCCGTTATCCGGGAAGAGGACAGTGTGTGGTGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGGGTAACGGAGGCGTTCAAAGTTACACTCAATACGGTCAGAAACCGTATCTAAGAGCATAAAGGTAGAAGTGTGATTGACTGTGAGACCTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCGGTTCTTCGTGGAAAGGCCGTCGCTCAACGGATAAAAGCTACCCCGGGGATAACAGGCTTATCTTTCCCAAGAGATCACATCGACGGGAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTTCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCTACTCTTAGTACGAGAGGACCGGAGTGGACGCACCGATGGTGTGCCAGTTGTTTCGCCAGAAGCATAGCTGGGTAGCCAAGTGCGGCAGGGATAACCGCTGAAAGCATCTAAGCGGGAAGCCCCCTCAAAGATTAGTATTCCCTTTAAAATTCCTTATAGACTATGAGGTTGATAGGCTGGAGGTGTAAGTGCAGCAATGCATTCAGCTGACCAGTACTAATAAATTTATTGGTTTAATAGT", "id": "AHCKFKLKGB_40", "locus": "GCA013367975_00569" }, { "type": "rRNA", "sequence": "contig_1", "start": 329655, "stop": 331172, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1513.4, "evalue": 2.3e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTATAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAGGTTAGCAATAACCTAGCGGCGAATGGGTGAGTAACACGTGCTTAATCTACCTTTTAGATTGGAATACCCATTGGAAACAATGGCTAATGCCGGATACGCATGGAACCGCATGGTTCCGTTGTGAAAGGCGCTGTAAGGCGCCACTAAAAGATGAGGGTGCGGAACATTAGTTAGTTGGTGAGGTAATGGCCCACCAAGACTATGATGTTTAGCCGGGTCGAGAGACTGAACGGCCACATTGGGACTGAGATACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCACGATGAAGGTCTTCGGATTGTAAAGTGCTGTTATAAGGGAAGAACATTTGCAATAGGAAATGATTGCAGACTGACGGTACCTTGTCAGAAAGCGATGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTTCGTAGGCTGTTTGTTAAGTCTGGAGTTAAATCCCGGGGCTCAACCCCGGCTCGCTTTGGATACTAGCAAACTAGAGTTAGATAGAGGTAAGCGGAATTCCATGTGAAGCGGTGAAATGCGTAGATATATGGAAGAACACCAAAGGCGAAGGCAGCTTACTGGGTCTATACTGACGCTGAGGGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTCGGTGGAGAATCACTGACGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACACGGAAAACCTTACCCACTCTTGACATCCTTCGCAAAGCTATAGAGATATAGTGGAGGTTATCGGAGTGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTGCAACGAGCGCAACCCCTATCTTTAGTTACTAACATTAAGTTGAGGACTCTAGAGATACTGCCTGGGTAACTGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCCACACACGTGCTACAATGGTCGGTACAAAGAGAAGCAATATGGCGACATGGAGCAAATCTCAAAAAGCCGATCTCAGTTCGGATTGGAGTCTGCAATTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCTATGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATACCCAAAGTCGGTTTGCTAACCTCGGAGGCGACCGCCTAAGGTAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "AHCKFKLKGB_35", "locus": "GCA013367975_00570" }, { "type": "cds", "sequence": "contig_1", "start": 331559, "stop": 331756, "strand": "-", "frame": 3, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "COG:COG0333", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_012855546.1", "SO:0001217", "UniParc:UPI0000DB8019", "UniRef:UniRef100_D1J858", "UniRef:UniRef50_Q4A6N8", "UniRef:UniRef90_D1J858" ], "nt": "ATGGCAGTAGTACCAAAAAGAAAAACTTCAAAACAAAGAAAACATTTAAGAAGATCACATCACGCTCTAGTGGCTCCAACATTAGTTGAATGTTCACAATGTAAGAATTTAATTACTCCTCACCAAGCATGTGAAAACTGCGGTTTTTATAGAGGAAGAAAAGTTATTAAAGAAGCAATTAACGATAAAATTAAATAA", "aa": "MAVVPKRKTSKQRKHLRRSHHALVAPTLVECSQCKNLITPHQACENCGFYRGRKVIKEAINDKIK", "aa_hexdigest": "2966d01971762bee811933c45cb0dcc6", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000DB8019", "ncbi_nrp_id": "WP_012855546.1", "uniref100_id": "UniRef100_D1J858", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000DB8019", "RefSeq:WP_012855546.1", "UniRef:UniRef100_D1J858" ] }, "ips": { "uniref100_id": "UniRef100_D1J858", "uniref90_id": "UniRef90_D1J858" }, "psc": { "uniref90_id": "UniRef90_D1J858", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_Q4A6N8", "cog_id": "COG0333", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N8" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "AHCKFKLKGB_585", "locus": "GCA013367975_00571" }, { "type": "cds", "sequence": "contig_1", "start": 331781, "stop": 332068, "strand": "-", "frame": 3, "gene": null, "product": "Large ribosomal subunit protein bL17", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20277" ], "nt": "TTGATTAATGGCAAAGTAACAACCACATTAGAACGTGCTAAAAGAATTCGTTCAAATGCAGAAAAAATGATTACTCTAGGTAAGAAAAATACCCTAGCTTCGCGTAGACAAGCAGCCAAATATTTAAGATTAATTGCAACAGAAAACAAAAATAAAAATTCATTACAATACTTATTTGATGTTGTGGCGCCAAAATACGTTGAACGTAATGGTGGATATACAAGAATTACAAAATTAGCCAATCGTGCTGGTGATAATGCTAAAATGGCAATAATCGAATTGGTATAA", "aa": "MINGKVTTTLERAKRIRSNAEKMITLGKKNTLASRRQAAKYLRLIATENKNKNSLQYLFDVVAPKYVERNGGYTRITKLANRAGDNAKMAIIELV", "aa_hexdigest": "624475da950116ad9799080f8a5457a3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FA24CA4", "query_cov": 1.063157894736842, "subject_cov": 0.808, "identity": 0.564, "score": 107.0, "evalue": 2.53e-28, "valid": false, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P20277", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P20277", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20277" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [], "id": "AHCKFKLKGB_586", "locus": "GCA013367975_00572" }, { "type": "cds", "sequence": "contig_1", "start": 332215, "stop": 333183, "strand": "-", "frame": 1, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_P38018", "UniRef:UniRef90_D1J860" ], "nt": "ATGAAGAAAATACAAAAAATTACATATAAAGAATTAGTAGCAGAAAAAGTATCAGATTTTAATACAACATTTGTTATTGAACCACTAATGCGTGGTTATGGTAATACAATAGGAACTGTAATAAGAAGAACATTACTTTCTTCAATTACTTCTGTTGCTCCATTTGCAATAAAAATTAGTCATGTTGAACATGAATTTACTGCAATTGAAGGATTAAGAGAAGATGCTATTACATTAGTTGCAAACATAAGAAAAATTCGCTTTGCATATAACCCAGAATTATTTGAAAAAGATAATCTAGCTAAAATTTCATTTAAATCAACAAAAGAAGGGGAAGTTCATGCTTCAGATATTGAAACTATCCCTGGACTTGACATTGTTAATAAAGATCAATACATAGCAACAATATCAAAAAATGGTTCATTGGAATTTGAATTATATTTAAGAACCGGTAGAGGCTATGTTTCATTTGAAGACAATAAAAAGACCATTCTAGAATATGGCCCAAAATTAGAATCAAAAATCAAAAATGGTCAATTTTTAGCAATGGATAGTGACTTCAGTCCTATTAAAAAAGTTGCGATTTTATTTGAAGAATTAAATTCTACTTCTAACATAATTGAAGAAAGATTAAAAATTAAAGTTGAAACCGATGGAACTGTTGAAGCTAAAAACGTTATGGAAGAAGCAGCAAAGATTATAGTTGCTCATTTCCAAATCATTGGAAACATTGACGCATTAGGAACAATTGATTTATTCGATGATCAAAAAGAAAAACATGAAAAAACTCCAAAAGTTTCAGTTTCTATTGATAAATTGAATTTGACAATTCGTTCATTGAATGCTTTAAGACGTGCTGGATTTAATAATGTTGATGAAATAATGAAATTAAGCGATGAAGAACTTTCAAATATTAAAAATTTGGGTAAAAAATCAGTACAAGATATTATTGACCGTCGTAGAGAATGA", "aa": "MKKIQKITYKELVAEKVSDFNTTFVIEPLMRGYGNTIGTVIRRTLLSSITSVAPFAIKISHVEHEFTAIEGLREDAITLVANIRKIRFAYNPELFEKDNLAKISFKSTKEGEVHASDIETIPGLDIVNKDQYIATISKNGSLEFELYLRTGRGYVSFEDNKKTILEYGPKLESKIKNGQFLAMDSDFSPIKKVAILFEELNSTSNIIEERLKIKVETDGTVEAKNVMEEAAKIIVAHFQIIGNIDALGTIDLFDDQKEKHEKTPKVSVSIDKLNLTIRSLNALRRAGFNNVDEIMKLSDEELSNIKNLGKKSVQDIIDRRRE", "aa_hexdigest": "87616d0f93ec027f09cd520c53e3717f", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J860", "query_cov": 1.0, "subject_cov": 0.936046511627907, "identity": 0.9940000000000001, "score": 606.0, "evalue": 2.68e-218, "valid": true, "gene": "RpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P38018", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P38018", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38018" ], "product": "DNA-directed RNA polymerase subunit alpha" }, "genes": [ "rpoA" ], "id": "AHCKFKLKGB_587", "locus": "GCA013367975_00573" }, { "type": "cds", "sequence": "contig_1", "start": 333477, "stop": 333587, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAGAAAAATAAAAAAGTTATTACTCAAGGTATTGCACATATTCACTCAACTTACCAAAACACAATTGTTTCTTTCTCAGATCTAAAAGGAAATGTTTTTGCTTGA", "aa": "MAKKNKKVITQGIAHIHSTYQNTIVSFSDLKGNVFA", "aa_hexdigest": "a34c7eb380a59aa586242df175c0d8bb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3990.5867000000007, "isoelectric_point": 10.000351905822754 }, "id": "AHCKFKLKGB_588", "locus": "GCA013367975_00574" }, { "type": "cds", "sequence": "contig_1", "start": 333590, "stop": 333958, "strand": "-", "frame": 3, "gene": "rpsM", "product": "30S ribosomal protein S13", "db_xrefs": [ "COG:COG0099", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02952", "RefSeq:WP_012855550.1", "SO:0001217", "UniParc:UPI0001BC2240", "UniRef:UniRef100_D1J862", "UniRef:UniRef50_P38017", "UniRef:UniRef90_D1J862" ], "nt": "ATGGCCAGAGTTTTAAACATTGAAATTCCTAATAATAAAAAAGTTCGTATTTCACTTACTTACATCTTTGGTATTGGCGTTTCACTAGCAAAAGAAATTTTAAAAGATGCTAATGTTGACGGCGAAAAAAGAGTTAAAGATCTTAGCGAAGAAGAATTAACTAGAATCAGAGATGAAGCTAAAAAATACACCACTGAAGGTGATTTAAGAAGAGAAATAAATTTAAACATTAAAAGATTAATGGAAATTAAATCATATCGTGGTATTAGACACCGTAAGGGATTACCTGTACGTGGTCAATCTACAAAGAAAAATGCAAGAACAAGAAAAGGTCCAAGAAAAACAATAGCAGGAAAGAAAGGTAAATAA", "aa": "MARVLNIEIPNNKKVRISLTYIFGIGVSLAKEILKDANVDGEKRVKDLSEEELTRIRDEAKKYTTEGDLRREINLNIKRLMEIKSYRGIRHRKGLPVRGQSTKKNARTRKGPRKTIAGKKGK", "aa_hexdigest": "ac04cc895c8f114982ffe618d49356fa", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2240", "ncbi_nrp_id": "WP_012855550.1", "uniref100_id": "UniRef100_D1J862", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2240", "RefSeq:WP_012855550.1", "UniRef:UniRef100_D1J862" ] }, "ips": { "uniref100_id": "UniRef100_D1J862", "uniref90_id": "UniRef90_D1J862" }, "psc": { "uniref90_id": "UniRef90_D1J862", "gene": "rpsM", "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_P38017", "cog_id": "COG0099", "cog_category": "J", "kegg_orthology_id": "K02952", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P38017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38017" ], "product": "Small ribosomal subunit protein uS13" }, "genes": [ "rpsM" ], "id": "AHCKFKLKGB_589", "locus": "GCA013367975_00575" }, { "type": "cds", "sequence": "contig_1", "start": 334087, "stop": 334302, "strand": "-", "frame": 1, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0006413", "KEGG:K02518", "RefSeq:WP_012855552.1", "SO:0001217", "UniParc:UPI0001BC2242", "UniRef:UniRef100_D1J864", "UniRef:UniRef50_Q4A5I6", "UniRef:UniRef90_D1J864" ], "nt": "ATGGCAAAAGACGCAATCAAAATGTCAGGCAAAATTACTAAAATGCACACATCAAGAGACTATGATGTTTTATTAGAAAATGGACTTACTATTAAAGCTTCTATTTCAGGTAAAATGTCACTTCACAATATCAAAATGATCCCTGGAGATTTAGTTGACGTTGAACTTAGTCCATATGATATGAGCAGAGGTAGGATTGTTTTTAGACACAAATAA", "aa": "MAKDAIKMSGKITKMHTSRDYDVLLENGLTIKASISGKMSLHNIKMIPGDLVDVELSPYDMSRGRIVFRHK", "aa_hexdigest": "00b621bb30ccf8e19c9059b63d700fad", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2242", "ncbi_nrp_id": "WP_012855552.1", "uniref100_id": "UniRef100_D1J864", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2242", "RefSeq:WP_012855552.1", "UniRef:UniRef100_D1J864" ] }, "ips": { "uniref100_id": "UniRef100_D1J864", "uniref90_id": "UniRef90_D1J864" }, "psc": { "uniref90_id": "UniRef90_D1J864", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q4A5I6", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5I6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5I6" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "AHCKFKLKGB_590", "locus": "GCA013367975_00576" }, { "type": "cds", "sequence": "contig_1", "start": 334401, "stop": 335054, "strand": "-", "frame": 2, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0006508", "GO:0070006", "SO:0001217", "UniRef:UniRef50_L0RW44", "UniRef:UniRef90_D1J865" ], "nt": "ATGGTCATAATAAAAAATCAATTTGAAATTGAAAAGATCAAAAAAGCTTGTAAAATCCTGGCAGAAGTGAAAACTGTTTTATACGACTTTATAAGACCAGGCGTTTCTTTAAAAGAAATTGATAGCGTAGCTTTTAAAGAAATAAGAAAAAGGGGTGGAAAACCAGCATTCTTAGGACAATATGGTTTCCCTAATACATGTTGCATTTCTGTTAATAATGAATTAATTCATGGAATTCCTAGTGATTATGTTGTAAAGGATGGAGATATAGTCAAAATTGACACTGGAGCAATTTATGACGGCTATTATTCTGATTCAGCATTTACAAAAGGAGTTGGCAACATAACTGAAGCAGACAAAAAATTAATTGCCTGCGCAAAAGACGCTTTTTATGCTGGCTTTAATGCAATTAAAGTTGGCAAACACATTGGTGATATTTCTGCAGCCATAGGAAATTTAATTCGTGATAGAGGATATTTTACTCCAGATGAATATACAGGTCACGGAATAGGCAGAAAGTTGCATGAAGATCCATATGTTTATAACGATGGCTATCCAGGTACTGGACAGCTAATTAGAAATGGTATGGTAATTTGCATTGAACCAATGATTTTACAAAAATCTAAAAATGTATTGGTCAAGGGAAATGGATGA", "aa": "MVIIKNQFEIEKIKKACKILAEVKTVLYDFIRPGVSLKEIDSVAFKEIRKRGGKPAFLGQYGFPNTCCISVNNELIHGIPSDYVVKDGDIVKIDTGAIYDGYYSDSAFTKGVGNITEADKKLIACAKDAFYAGFNAIKVGKHIGDISAAIGNLIRDRGYFTPDEYTGHGIGRKLHEDPYVYNDGYPGTGQLIRNGMVICIEPMILQKSKNVLVKGNG", "aa_hexdigest": "f0771c603bdc7f9bf4e85fd5e65ecb54", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_D1J865", "query_cov": 1.0, "subject_cov": 0.868, "identity": 1.0, "score": 442.0, "evalue": 1.02e-156, "valid": true, "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_L0RW44", "cog_id": "COG0024", "cog_category": "J", "go_ids": [ "GO:0006508", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW44" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "AHCKFKLKGB_591", "locus": "GCA013367975_00577" }, { "type": "cds", "sequence": "contig_1", "start": 335045, "stop": 335740, "strand": "-", "frame": 3, "gene": "adk", "product": "adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0005524", "GO:0006139", "GO:0019205", "RefSeq:WP_036439187.1", "SO:0001217", "UniParc:UPI000512ED24", "UniRef:UniRef100_A0A2K9YTD3", "UniRef:UniRef50_Q6KI35", "UniRef:UniRef90_D1J866" ], "nt": "ATGATAGAAAAATCTCAAGAAGTGTACAACGAACACGCAAAAAAATTTCCTAATATAATTATGCTTGGCGCACCGGGAGCTGGTAAAGGCTCTATTGCAACAAAAATGGTGCAAAATAATGATTATGTTCAAATTTCAACTGGGGACATGTTTAGACAAGAAATTAAAAATCAAACTCCCCTTGGCTTAAAAATAAAATCTATTTTAGATTCGGGAGCATATGTTGATGATTCTATAACAAATCAACTAGTTGAAAAAAAATTAACTGAATTAATAAGAGAAAAGAAACACTTCATATTAGACGGATATCCCCGCACATTAGCTCAAGCAAAGTTTTTACTTTCATTAGCAAATGTTGGAATTAAAATTGATAAAGTAATTTTACTAGAAATTACTGATGAACAAATTATTGACCGTCTTTCAAAACGTAGAATTTGTTTAAATTGTAAAACCATTTATCATTTGCAATCATTTCCACCATTACCTGGTGATTTGTGTATTAAATGCCACGAAAAAGTAACAAAAAGACCAGATGACGAACCAGAAGTAATAAAGAAACGTTTATCAATATATAATGAACAAACTAAATGTTTGATCAATTTTTATCAAGAACTTGGTATACTTTTAAAGATAAATAGTTATCAAACGATTGATAAAGTATATAATGATGTAAAAGAGGCTTTAAAATGGTCATAA", "aa": "MIEKSQEVYNEHAKKFPNIIMLGAPGAGKGSIATKMVQNNDYVQISTGDMFRQEIKNQTPLGLKIKSILDSGAYVDDSITNQLVEKKLTELIREKKHFILDGYPRTLAQAKFLLSLANVGIKIDKVILLEITDEQIIDRLSKRRICLNCKTIYHLQSFPPLPGDLCIKCHEKVTKRPDDEPEVIKKRLSIYNEQTKCLINFYQELGILLKINSYQTIDKVYNDVKEALKWS", "aa_hexdigest": "3cde7b7fa6f09b103478ebb4e4f163fd", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI000512ED24", "ncbi_nrp_id": "WP_036439187.1", "uniref100_id": "UniRef100_A0A2K9YTD3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512ED24", "RefSeq:WP_036439187.1", "UniRef:UniRef100_A0A2K9YTD3" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTD3", "uniref90_id": "UniRef90_D1J866" }, "psc": { "uniref90_id": "UniRef90_D1J866", "gene": "Adk", "product": "adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q6KI35", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005524", "GO:0006139", "GO:0019205" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KI35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KI35" ], "product": "Adenylate kinase" }, "genes": [ "adk" ], "id": "AHCKFKLKGB_592", "locus": "GCA013367975_00578" }, { "type": "cds", "sequence": "contig_1", "start": 335737, "stop": 336168, "strand": "-", "frame": 1, "gene": "secY", "product": "SecY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD3D78", "UniRef:UniRef90_UPI0021BD3D78" ], "nt": "TTGCAATTTACTCAACCACTTGGATTCTTTCTATTTATTTTAATTACATTTGGATTAACAATATTAATGGGAATCCAACAATCGAGAATTGATAAAATTAGTGAAGATTTTGCTAAAAATAGTACTTTTATTCCTGGCGTTAGACCCGGAGAACAAACAGAAGATTATTTATTGAATGTAATTTTACGTTTATCATTCTTTTCTTCAGGATATTTATTGCTGCTAGGCGCTTTGCAATTTATTCAACAAATGCTAGGCATGCCAGCCTCAATAGCATTTGGCGGTACAAGCATAATGATACTAGTTTCAACGGCTTATGAAACTGTTCAACAAATTAAAGCCCGCTACAAGTCACAAGAATTATCAAGAAAGAGAAAAATGATAAGAGAATTAAAAGAAATGTATGGAGAGGAAGAAGAAGATTTAATATGA", "aa": "MQFTQPLGFFLFILITFGLTILMGIQQSRIDKISEDFAKNSTFIPGVRPGEQTEDYLLNVILRLSFFSSGYLLLLGALQFIQQMLGMPASIAFGGTSIMILVSTAYETVQQIKARYKSQELSRKRKMIRELKEMYGEEEEDLI", "aa_hexdigest": "85abb75e04e5391b92af8f76c1e9a417", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "secY" ], "truncated": "3-prime", "pseudogene": { "start": 335737, "stop": 336468, "observations": { "mutation": [ 336183 ], "stop": [ 336183 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021BD3D78", "query_cov": 1.0, "subject_cov": 0.5296296296296297, "identity": 0.867, "score": 243.0, "evalue": 5.13e-79, "gene_start": 1, "gene_end": 143, "reference_start": 127, "reference_end": 269, "reference_sequence": "MLWFFVGIASGLIPSFKSAFQYYIPSAKQTNLVLQEIINYAVYILVYLLTIFIVVIFTIAERRIPIQQVGAGLSKNAEELSYLPLKANPAGIMSVIFSMMILGVPTMIANMLNPNTSRYYQWVYANLQFTQPLGFFLFILITFGLTILMGIQQSKIDKISEDFAKSSTYIPGIRPGEQTETYLLNVVLRLSFFSSGFLIVLGSLQFVQQMIGMPASIAFGGTTVMILVSTAYETIQQIKARYKSQELARKRRTIRELKEMYGEEEEDLIW" }, "paralog": false, "identity": 0.8559670781893004, "score": 414.0, "evalue": 5.05e-149, "description": "Internal stop codon at 336183. Nonsense mutation around 336183.", "psc": { "uniref90_id": "UniRef90_UPI0021BD3D78", "product": "SecY", "uniref50_id": "UniRef50_UPI0021BD3D78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021BD3D78", "UniRef:UniRef50_UPI0021BD3D78" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021BD3D78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD3D78" ] } }, "id": "AHCKFKLKGB_593", "locus": "GCA013367975_00579" }, { "type": "cds", "sequence": "contig_1", "start": 336181, "stop": 336954, "strand": "-", "frame": 1, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952", "SO:0001217", "UniRef:UniRef50_A0A448ZXS5", "UniRef:UniRef90_D1J867" ], "nt": "TTGAATTTAGTAGGTGGCGGCGGATTAAGACAATTCTCTGTTGTCGCGCTAGGAATAGGACCGTTTATATCATCTAGTTTAGTTATGATGATATTGCAAACTAAAGCATTCCCAGCCATTCACCGTTTAAGTCAATCTGGTCCTCAAGGCCGGATTAAAATAAATTTCATTACTTACGGAGTTACTTTTGTTTTTGCAATAGTTCAAGCATATTTGATAACAAAAGCATTAGTTAATCCTCGTCAAGGATTTGGAATTACTTTTTCACCAAAATTAGAAAAATTATTTGGACAAAGTGGAATGGTGGCATATCAATATTTCATTTTGCCTGCAATATTAGTAGCTGGCTCATTCTTTTCATTGTTTTTATCAGAACAAATAACTAATAGAGGCGTTGGCAATGGAACGAGTTTAATAATCTTTGTTGGTATAGCAAGTGCTTTAATTCCTTCATTCAAGAGTGCATTTAAATTTTATGTTCCATCAGCTCAACAAAATAATTTAGTTCTACAAGAAGCAATAAATTACGCAGTATATATCTTTTGTTATTTACTTGTAATATTTATTGTTGTAATATTTACAATTGCAGAAAGACATATTCCAATTCAACAAGTTGGTGCGGGTCTTTCTAAAAACGAAAAAGAATTGAGTTATTTACCTATTAAAGCAAATCCTGCTGGTATCATGTCAGTTATCTTCTCAATGATGATATTGAGTGTGCCAACAATGATTGCAAACTTATTTGATCCAAATACAAGCAAGTATTATCAATGA", "aa": "MNLVGGGGLRQFSVVALGIGPFISSSLVMMILQTKAFPAIHRLSQSGPQGRIKINFITYGVTFVFAIVQAYLITKALVNPRQGFGITFSPKLEKLFGQSGMVAYQYFILPAILVAGSFFSLFLSEQITNRGVGNGTSLIIFVGIASALIPSFKSAFKFYVPSAQQNNLVLQEAINYAVYIFCYLLVIFIVVIFTIAERHIPIQQVGAGLSKNEKELSYLPIKANPAGIMSVIFSMMILSVPTMIANLFDPNTSKYYQ", "aa_hexdigest": "36e165d8b8faf321a6d6ce6ece0a03ce", "start_type": "TTG", "rbs_motif": "AGGAG(G)/GGAGG", "genes": [ "secY" ], "truncated": "both", "pseudogene": { "start": 335878, "stop": 337203, "observations": { "mutation": [ 336183, 337095, 337098, 337107 ], "stop": [ 336183, 337095, 337098, 337107 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J867", "query_cov": 1.0, "subject_cov": 0.5255623721881391, "identity": 0.996, "score": 489.0, "evalue": 4.52e-171, "gene_start": 1, "gene_end": 257, "reference_start": 84, "reference_end": 340, "reference_sequence": "MARKTKLEKEISQEKIERKLALDKFLVDKKNTWNEWWKNHNLFKKILFTLFIITLFLATGTITIPGVNLANPDSLTQGDFFGILNLVGGGGLRQFSVVALGIGPFISSSLVMMILQTKAFPAIHRLSQSGPQGRIKINFITYGVTFVFAIVQAYLITKALVNPRQGFGITFSPKLEKLFGQSGMVAYQYFILPAILVAGSFFSLFLSEQITNRGVGNGTSLIIFVGIASALIPSFKSAFKFYVPSAQQNNLVLQEAINYAVYIFCYLLVIFIVVIFTIAERHIPIQQVGAGLSKNEKELSYLPIKANPAGIMSVIFSMMILSVPTMIANLFDPNTSKYYQWVYNNLQFTQPLGFFLFILITFGLTILMGIQQSRIDKISEDFAKNSTFIPGVRPGEQTEDYLLNVILRLSFFSSGYLLLLGALQFIQQMLGMPASIAFGGTSIMILVSTAYETVQQIKARYKSQELSRKRKMIRELKEMYGEEEEDLIW" }, "paralog": false, "identity": 0.9909297052154195, "score": 839.0, "evalue": 2.36e-311, "description": "Internal stop codon at 336183, 337095, 337098, 337107. Nonsense mutation around 336183, 337095, 337098, 337107.", "psc": { "uniref90_id": "UniRef90_D1J867", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_A0A448ZXS5", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0015031", "GO:0016020", "GO:0043952" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J867", "UniRef:UniRef50_A0A448ZXS5", "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXS5" ], "product": "Protein translocase subunit SecY" } }, "id": "AHCKFKLKGB_594", "locus": "GCA013367975_00580" }, { "type": "cds", "sequence": "contig_1", "start": 337105, "stop": 337203, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGAAAAACTAAGTTGGAAAAAGAGATTAGTCAAGAAAAGATCGAGCGCAAATTAGCACTAGATAAGTTTTTAGTTGATAAAAAAAACACTTGA", "aa": "MARKTKLEKEISQEKIERKLALDKFLVDKKNT", "aa_hexdigest": "52b053d98925f993c9317f2b09f4dc46", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3832.5150000000012, "isoelectric_point": 9.775808525085449 }, "id": "AHCKFKLKGB_595", "locus": "GCA013367975_00581" }, { "type": "cds", "sequence": "contig_1", "start": 337203, "stop": 337637, "strand": "-", "frame": 2, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_012855556.1", "SO:0001217", "UniParc:UPI0001BC2246", "UniRef:UniRef100_D1J868", "UniRef:UniRef50_Q601J6", "UniRef:UniRef90_D1J868" ], "nt": "ATGCAATTACATAATTTAAAACCAACTCCAGGGGCAAGAAAAGACAAACACCGTGTTGGTCGTGGTCATGCTGCTGGAAAAGGAAAACAAGCCGGTAGAGGACAAAGTGGTCAAACAAAAAGAAGCAAAGTAAGACTAGGATTTGAAGGTGGCCAATTGCCACTATTTAGAAGAATTCCAAAACGTGGATTTAGCAATGTAAATCACGTTGAATTCCAAGTAGTAAATCTAGCAGATTTACAATCAAAATATCAAGATGGTGAAGTAGTTTCTTACGAAACACTTGCTGCAAGAAACTTGATTAAAGGAACATTACCAGTTAAAATTTTAGCTAAGGGTTCTTTAACTAAAAAACTTAATGTACAAATTCCAACACTTTCACAATCCGCTATTGAAGCTATTGAAAAAGTCGGAGGAAAAATAGAGGTTAAATAA", "aa": "MQLHNLKPTPGARKDKHRVGRGHAAGKGKQAGRGQSGQTKRSKVRLGFEGGQLPLFRRIPKRGFSNVNHVEFQVVNLADLQSKYQDGEVVSYETLAARNLIKGTLPVKILAKGSLTKKLNVQIPTLSQSAIEAIEKVGGKIEVK", "aa_hexdigest": "e48e82061d297ab366d048e8a4e1e848", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2246", "ncbi_nrp_id": "WP_012855556.1", "uniref100_id": "UniRef100_D1J868", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2246", "RefSeq:WP_012855556.1", "UniRef:UniRef100_D1J868" ] }, "ips": { "uniref100_id": "UniRef100_D1J868", "uniref90_id": "UniRef90_D1J868" }, "psc": { "uniref90_id": "UniRef90_D1J868", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_Q601J6", "cog_id": "COG0200", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q601J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q601J6" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "AHCKFKLKGB_596", "locus": "GCA013367975_00582" }, { "type": "cds", "sequence": "contig_1", "start": 337647, "stop": 338315, "strand": "-", "frame": 2, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02988", "RefSeq:WP_020002417.1", "SO:0001217", "UniParc:UPI000367CA6D", "UniRef:UniRef100_A0A454CA16", "UniRef:UniRef50_L0RWI9", "UniRef:UniRef90_D1J869" ], "nt": "ATGGCTGAAGTAGAAAAAAATATTAAAAATTCAGAAAAACCTGAATTAAAAGAAAATAAAACAGAAGGCACAAAGGTTGTTTCTTTTAAAAGAGAAAGATCAAATTCAAAAGATCTAAAATCAAGCGAAAATAAACCAAAACAATTCCAAAATCGTAATCAAGATCATTCAGCAAATCAAGCTCAAGCAAAACAATTTGAAGAAAAAGTTATTGACATTGCAAGAGTTACAACTGTTGTTAAGGGTGGACGTAGATTTTCATTCTCAGCTTATGTTGTTGTTGGTGACAAAAAAGGAAAAGTTGGATTTGGACACGGAAAAGCTAATGAAGTCCAAGATGCAATTAAAAAAGCAGTTAAAGATGCACAAAAAAATGTTTTCTCAGTTCCAATTTCAAATGGAACAATCCCTCATGAAATCCAAGATAAATTCTTAGCATCAAGAATCCAATTACGTCCAGCTCCAAAAGGTGCCGGAATCATTGCTTCAAACACCGTTCGTGCTGTTGTTGAATTAGCAGGTTACACCGACATTTCAACAAAAACTTATGGTTCAAGAACAAAACAAAACATTGTCCAAGCTACTATTAAGGCACTTAAAAAAGTTAGAACTGTTGAAGAAATCGCAAAATTACGCGATATTGATGTAAATCATCTATTATCAAAATAA", "aa": "MAEVEKNIKNSEKPELKENKTEGTKVVSFKRERSNSKDLKSSENKPKQFQNRNQDHSANQAQAKQFEEKVIDIARVTTVVKGGRRFSFSAYVVVGDKKGKVGFGHGKANEVQDAIKKAVKDAQKNVFSVPISNGTIPHEIQDKFLASRIQLRPAPKGAGIIASNTVRAVVELAGYTDISTKTYGSRTKQNIVQATIKALKKVRTVEEIAKLRDIDVNHLLSK", "aa_hexdigest": "bcbc4611471dda464e1e9604f871d769", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI000367CA6D", "ncbi_nrp_id": "WP_020002417.1", "uniref100_id": "UniRef100_A0A454CA16", "db_xrefs": [ "SO:0001217", "UniParc:UPI000367CA6D", "RefSeq:WP_020002417.1", "UniRef:UniRef100_A0A454CA16" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CA16", "uniref90_id": "UniRef90_D1J869" }, "psc": { "uniref90_id": "UniRef90_D1J869", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_L0RWI9", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_L0RWI9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RWI9" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "AHCKFKLKGB_597", "locus": "GCA013367975_00583" }, { "type": "cds", "sequence": "contig_1", "start": 338315, "stop": 338662, "strand": "-", "frame": 3, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "COG:COG0256", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855558.1", "SO:0001217", "UniParc:UPI0001BC2248", "UniRef:UniRef100_D1J870", "UniRef:UniRef50_A0A6M4JHS6", "UniRef:UniRef90_D1J870" ], "nt": "ATGTTATCAAGAAATTTAAAACGTAAAGCAAAACACTTAAAGATTACTAACAAATTGTCAAAAGGAACTGCATTAGTTCCTAGAGTTGTTGTTTTTAAATCTTTACATGCATTTTATGCACAAGCAGTAAACGATGAATTGCACACTACAATTGCTTCATCATCAACACAAATACTAGAAAATAAAGCAAACAATATTGATGCAGTTAAATTAGTTGCAAAAGATTTTGCTAAAAAATTATTGGCAATCAATGTAAAATCAATAGTGTTTGATCGTTCAGGATACATATACCACGGAAAATTAGCAGCTTTTGCTGATACTCTAAGAGAAGAAGGGATTAAATTCTAA", "aa": "MLSRNLKRKAKHLKITNKLSKGTALVPRVVVFKSLHAFYAQAVNDELHTTIASSSTQILENKANNIDAVKLVAKDFAKKLLAINVKSIVFDRSGYIYHGKLAAFADTLREEGIKF", "aa_hexdigest": "4512354e3b284c6d5db3f37ab085dddf", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2248", "ncbi_nrp_id": "WP_012855558.1", "uniref100_id": "UniRef100_D1J870", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2248", "RefSeq:WP_012855558.1", "UniRef:UniRef100_D1J870" ] }, "ips": { "uniref100_id": "UniRef100_D1J870", "uniref90_id": "UniRef90_D1J870" }, "psc": { "uniref90_id": "UniRef90_D1J870", "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_A0A6M4JHS6", "cog_id": "COG0256", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6M4JHS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4JHS6" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "AHCKFKLKGB_598", "locus": "GCA013367975_00584" }, { "type": "cds", "sequence": "contig_1", "start": 338684, "stop": 339223, "strand": "-", "frame": 3, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "RefSeq:WP_012855559.1", "SO:0001217", "UniParc:UPI0001BC2249", "UniRef:UniRef100_D1J871", "UniRef:UniRef50_Q4A5D6", "UniRef:UniRef90_D1J871" ], "nt": "ATGTCTAGAATTGGAAAAAGAGTTTTAGAAATACCAAAAGATGTTGAAGTTAAATTAGATAAAAACCATATCGAAGTAAAAGGCAAATTAGGAGAATTGAGTTATACATTCTCACCATTAATTTCAGTAGTTGTTGAAAACAACGAAATTAAGACATTGAGATCTAACGAAGAAAAATCTACAAAACAATTACACGGAACAACAAATGCAAACATTAAAAATATGTTAATAGGTGTTTCAAGTGGTTACAAAAAAGAAATTGAAATTAAAGGTGTAGGTTACAAAGCTACCTTAAAGGGAAATACAATTGAAATTATTGCTGGTTATTCACATGCTGTAAATTTAGAAGTTCCATCAAATCTAAAAATTGAATTACCAAAACCAACCAATATTATTATTTCAGGAATTGACAAACAAGCTGTTGGTGAATTTGCTGCAAATATTCGTGATGTAAGACGTCCAAGTCCATATTCTGGAAAAGGTATTATGTATAAAGATGAAGTTATTAGACGTAAGGAAGGAAAGACAGCTGCTAAATAG", "aa": "MSRIGKRVLEIPKDVEVKLDKNHIEVKGKLGELSYTFSPLISVVVENNEIKTLRSNEEKSTKQLHGTTNANIKNMLIGVSSGYKKEIEIKGVGYKATLKGNTIEIIAGYSHAVNLEVPSNLKIELPKPTNIIISGIDKQAVGEFAANIRDVRRPSPYSGKGIMYKDEVIRRKEGKTAAK", "aa_hexdigest": "e98ddf5827d0489bd840bf7f811c766a", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001BC2249", "ncbi_nrp_id": "WP_012855559.1", "uniref100_id": "UniRef100_D1J871", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2249", "RefSeq:WP_012855559.1", "UniRef:UniRef100_D1J871" ] }, "ips": { "uniref100_id": "UniRef100_D1J871", "uniref90_id": "UniRef90_D1J871" }, "psc": { "uniref90_id": "UniRef90_D1J871", "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_Q4A5D6", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5D6" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "AHCKFKLKGB_599", "locus": "GCA013367975_00585" }, { "type": "cds", "sequence": "contig_1", "start": 339248, "stop": 339658, "strand": "-", "frame": 3, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_Q6MSN9", "UniRef:UniRef90_D1J872" ], "nt": "ATGGCTATTATTATAGATCCAGTTGCAGATATGTTTGTACGTATTAAAAATGCAATTTCTCGTAAATATCATGAAGTTGTATTGCCACATTCAAACAAGAAAGCAAAAATTTTAGAAATTATTAAAAGAGAAGGATATATTGTTGATTTTGAAGTTGTAAAAAACGAAAAAACCAATTTTAAAGAAATAAAAGTTACTTTAAAATACAAAGGTATGAATCAAAATCAATCAGCAATTTCTGGTCTTAAAAAAGTTTCAAAACCTGGCTTAAAAGTATATTCATCTGCTGAAAAATTGCCTAGAGTTTTAAGTGGATATGGAACAGCTATTATTTCAACATCAAAAGGTTTATTAACAGATAAAGAAGCTCGTAAGGCTAATGTTGGTGGCGAAGTTATCGCCTTCATTTGA", "aa": "MAIIIDPVADMFVRIKNAISRKYHEVVLPHSNKKAKILEIIKREGYIVDFEVVKNEKTNFKEIKVTLKYKGMNQNQSAISGLKKVSKPGLKVYSSAEKLPRVLSGYGTAIISTSKGLLTDKEARKANVGGEVIAFI", "aa_hexdigest": "44621eacfa9ce74a148bdad8879e46d6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J872", "query_cov": 1.0, "subject_cov": 0.9927007299270073, "identity": 1.0, "score": 256.0, "evalue": 4.4e-86, "valid": true, "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q6MSN9", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MSN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MSN9" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "AHCKFKLKGB_600", "locus": "GCA013367975_00586" }, { "type": "cds", "sequence": "contig_1", "start": 339664, "stop": 339846, "strand": "-", "frame": 1, "gene": null, "product": "Small ribosomal subunit protein uS14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP7" ], "nt": "ATGGCAAGAACATCATTAAAGGTTAAAGCCGAACGCCAACCTAAATTTAAGGTTAGAAAATATACTCGTTGCCAACTTTGTGGCCGTGTTCACGCTGTATTGCGTAAATATAAAATTTGTAGAATTTGTTTTAGAAAATTAGCACATGAAGGAAAACTTCCTGGTGTTAAGAAAGCGAGCTGA", "aa": "MARTSLKVKAERQPKFKVRKYTRCQLCGRVHAVLRKYKICRICFRKLAHEGKLPGVKKAS", "aa_hexdigest": "4b14d9dfdd9d224ff7a908e546cd8f55", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A448ZVU2", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 0.85, "score": 106.0, "evalue": 2.85e-29, "valid": false, "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_Q9PQP7", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP7" ], "product": "Small ribosomal subunit protein uS14" }, "genes": [], "id": "AHCKFKLKGB_601", "locus": "GCA013367975_00587" }, { "type": "cds", "sequence": "contig_1", "start": 340173, "stop": 340418, "strand": "-", "frame": 2, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "nt": "ATGGCAAAACAAATTAAATTAGCATTAGAACAAAAATATTTAGACGAAATTCGTTTCGCGCTAGTTAAAGAATTTGGTTACACCAGTGTAATGCAAGCTCCTAAACTAGAAAAAATCGTTATTAACATGACTGCCGGTAACGAAGTTTCAAACTCAAAGGCAATCGAAGAAGTTATGGTTGAACTTGCTCAAATTACAGGTCAAAAACCATATCAAACAACTGCTAAGAAATCATTGGCTTCATGA", "aa": "MAKQIKLALEQKYLDEIRFALVKEFGYTSVMQAPKLEKIVINMTAGNEVSNSKAIEEVMVELAQITGQKPYQTTAKKSLAS", "aa_hexdigest": "7a5beec12a4a6cd8a099cdf0e8effdc6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002E1CF638", "query_cov": 0.8888888888888888, "subject_cov": 0.8571428571428571, "identity": 0.569, "score": 79.7, "evalue": 5.85e-18, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A2X3HCF5", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A2X3HCF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "AHCKFKLKGB_602", "locus": "GCA013367975_00588" }, { "type": "cds", "sequence": "contig_1", "start": 340496, "stop": 340768, "strand": "-", "frame": 3, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_A0A448ZW26", "UniRef:UniRef90_A0A454CAH0" ], "nt": "ATGGCTCAAGCTAAAATTAGAAAAAATGACATGGTATTAATTATTGCTGGTAAAGACAAAGGAAAATTTGCTGCTGTTTTATCAACAAATCAAAAAGAACAAACTGTTGTTTTAAAAGGCGTAAACATTAAAACTAAACACCACAGACCATCACAAGAAAATAATGATGGAAAAATTGAAAAGAAAGAATACCCAATTCATATTTCAAACGTTGCTTATTTGGTTAAAAAAGGTGCTCAAGGTCAAAAATCAATCGGTTCAAAAATTGGTTGA", "aa": "MAQAKIRKNDMVLIIAGKDKGKFAAVLSTNQKEQTVVLKGVNIKTKHHRPSQENNDGKIEKKEYPIHISNVAYLVKKGAQGQKSIGSKIG", "aa_hexdigest": "a74a670b853a52000f9ce7c62c8ae22b", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_A0A454CAH0", "query_cov": 1.0, "subject_cov": 0.8108108108108109, "identity": 0.9890000000000001, "score": 172.0, "evalue": 2.48e-54, "valid": true, "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_A0A448ZW26", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZW26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZW26" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "AHCKFKLKGB_603", "locus": "GCA013367975_00589" }, { "type": "cds", "sequence": "contig_1", "start": 340770, "stop": 341138, "strand": "-", "frame": 2, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02874", "RefSeq:WP_012855564.1", "SO:0001217", "UniParc:UPI0001BC229F", "UniRef:UniRef100_D1J876", "UniRef:UniRef50_A5FMZ0", "UniRef:UniRef90_D1J876" ], "nt": "ATGCTTAGTGAATTTTCAAGATGTAACGTTGCTGACAATTCAGGCGCAAAAGAAGTTATGATTATTAAAAACCTAGGTGGATCAATTGTTAGATCAACCAATATTGGTGATATTGTTGTTGTAACTATTAAAAAAGCTATTCCAAATGGAATTGTAAAAGAAGGCCAAGTTGTTAAAGCAGTTATAGTTAGAACAAAACGTGGTTTAATGCGTGAAAATGGTTCTCATATAAGATTTGATGATAATGCTGTTGTATTAATTAAGGAAGATAACTCATTAAGAGGAACTCGGGTATTTGGTCCAGTAGCAAGAGAATTACGTGAAAAAGGTTTTTTAAAGATTGTTTCATTAGCTCCGGAGGTTTTATAA", "aa": "MLSEFSRCNVADNSGAKEVMIIKNLGGSIVRSTNIGDIVVVTIKKAIPNGIVKEGQVVKAVIVRTKRGLMRENGSHIRFDDNAVVLIKEDNSLRGTRVFGPVARELREKGFLKIVSLAPEVL", "aa_hexdigest": "c87871ee0223ca5673069e09b0bae576", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC229F", "ncbi_nrp_id": "WP_012855564.1", "uniref100_id": "UniRef100_D1J876", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC229F", "RefSeq:WP_012855564.1", "UniRef:UniRef100_D1J876" ] }, "ips": { "uniref100_id": "UniRef100_D1J876", "uniref90_id": "UniRef90_D1J876" }, "psc": { "uniref90_id": "UniRef90_D1J876", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_A5FMZ0", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A5FMZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5FMZ0" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "AHCKFKLKGB_604", "locus": "GCA013367975_00590" }, { "type": "cds", "sequence": "contig_1", "start": 341131, "stop": 341415, "strand": "-", "frame": 1, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "COG:COG0186", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855565.1", "SO:0001217", "UniParc:UPI0001BC22A0", "UniRef:UniRef100_D1J877", "UniRef:UniRef50_Q98PZ1", "UniRef:UniRef90_D1J877" ], "nt": "ATGGAAACTACAGTTAGAGAAAATCGTCGTAAAACTTTAGTTGGAACAGTTGTATCAACTAAAAACCAAAAAACAATTACTGTTGTTGTAGAAATTTACGAAAAACACCCTCTTTATTCAAAACGTTTTAAAAAATCAAAGAAATTTGCAGTTCATGATGAACAAAATTTAGCTAAAGTTGGTGACATTGTTAAAATTCAAGAAACAAGACCTTTATCAAAAACCAAACACTTTAGACTAGTAGAAATCAAATCACACGCAATTGAAGGAGCAGAAAATGCTTAG", "aa": "METTVRENRRKTLVGTVVSTKNQKTITVVVEIYEKHPLYSKRFKKSKKFAVHDEQNLAKVGDIVKIQETRPLSKTKHFRLVEIKSHAIEGAENA", "aa_hexdigest": "ea20c26ef5b5db251866ea7f325bed04", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001BC22A0", "ncbi_nrp_id": "WP_012855565.1", "uniref100_id": "UniRef100_D1J877", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A0", "RefSeq:WP_012855565.1", "UniRef:UniRef100_D1J877" ] }, "ips": { "uniref100_id": "UniRef100_D1J877", "uniref90_id": "UniRef90_D1J877" }, "psc": { "uniref90_id": "UniRef90_D1J877", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_Q98PZ1", "cog_id": "COG0186", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PZ1" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "AHCKFKLKGB_605", "locus": "GCA013367975_00591" }, { "type": "cds", "sequence": "contig_1", "start": 341415, "stop": 341615, "strand": "-", "frame": 2, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855566.1", "SO:0001217", "UniParc:UPI0001BC22A1", "UniRef:UniRef100_D1J878", "UniRef:UniRef50_A0A3B0PQ96", "UniRef:UniRef90_D1J878" ], "nt": "ATGACATTTGCAGAATTAAAAGAAAAATCACTTGACGAACTAAATAAGTTGGTTGCTGATTTAAGAGCAGAACTATTCTCATTACGTTTCAAAAATGCTACAAGACAATTAGACGAAACCCACAAAATCCAATTAGTTAAAAAAGATATCGCAAGAACTTTAACAGCAATTAAAGCAAAAACTTTAGAAGGGAATAAATAA", "aa": "MTFAELKEKSLDELNKLVADLRAELFSLRFKNATRQLDETHKIQLVKKDIARTLTAIKAKTLEGNK", "aa_hexdigest": "cb17ed1367064946a7cec20043621673", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A1", "ncbi_nrp_id": "WP_012855566.1", "uniref100_id": "UniRef100_D1J878", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A1", "RefSeq:WP_012855566.1", "UniRef:UniRef100_D1J878" ] }, "ips": { "uniref100_id": "UniRef100_D1J878", "uniref90_id": "UniRef90_D1J878" }, "psc": { "uniref90_id": "UniRef90_D1J878", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_A0A3B0PQ96", "cog_id": "COG0255", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PQ96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PQ96" ], "product": "Large ribosomal subunit protein uL29" }, "genes": [ "rpmC" ], "id": "AHCKFKLKGB_606", "locus": "GCA013367975_00592" }, { "type": "cds", "sequence": "contig_1", "start": 341789, "stop": 341896, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00252.24" ], "nt": "ATGGGACGTGAAGGAAAAGTTATTATTAAAATATTCCCTCACATGTCAAAAACTAGTAAACCTATTGGTGTTCGTATGGGATCTGGTAAGGGTAGCCCAGATCAATGA", "aa": "MGREGKVIIKIFPHMSKTSKPIGVRMGSGKGSPDQ", "aa_hexdigest": "06b42c6d46d5e2a7d1d7dd09ca724493", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00252.24", "name": "Ribosomal_L16", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 3.365331606633612e-10, "score": 35.33918762207031, "start": 3, "stop": 34 } ], "seq_stats": { "molecular_weight": 3755.4388000000017, "isoelectric_point": 10.564772605895993 }, "id": "AHCKFKLKGB_607", "locus": "GCA013367975_00593" }, { "type": "cds", "sequence": "contig_1", "start": 341945, "stop": 342070, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCAACCAAAAAGAACTAAACATAGAAAAATGTTCCGTATCCGCCACGACAAAGTTGTTGCACACCGTAATAATACAGTTGCATTTGGCGAATTTGGTTTAAAATCAACTTCATCAGCATGA", "aa": "MLQPKRTKHRKMFRIRHDKVVAHRNNTVAFGEFGLKSTSSA", "aa_hexdigest": "7c21f6da5b3d095a3e3e708e02acb1c9", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4781.533299999998, "isoelectric_point": 11.849047279357908 }, "id": "AHCKFKLKGB_608", "locus": "GCA013367975_00594" }, { "type": "cds", "sequence": "contig_1", "start": 342165, "stop": 342284, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00189.25" ], "nt": "ATGGCTAGAACAGAAGGATATACCGAAGGTGAAATGAAATTACATACTTTAAGACAAGATGTTGATTATGCCGTAACAACAGCCTTAACAACATATGGTATCTTAGGAGTTAAGGTATGA", "aa": "MARTEGYTEGEMKLHTLRQDVDYAVTTALTTYGILGVKV", "aa_hexdigest": "1f121bf5c4c5cbf158767497bb45b9d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00189.25", "name": "Ribosomal_S3_C", "length": 39, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.2778757313523623e-15, "score": 53.091026306152344, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 4332.9064, "isoelectric_point": 5.53852787017822 }, "id": "AHCKFKLKGB_609", "locus": "GCA013367975_00595" }, { "type": "cds", "sequence": "contig_1", "start": 342779, "stop": 343168, "strand": "-", "frame": 3, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "COG:COG0091", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02890", "RefSeq:WP_012855569.1", "SO:0001217", "UniParc:UPI0001BC22A4", "UniRef:UniRef100_Q8GM55", "UniRef:UniRef50_Q98PY6", "UniRef:UniRef90_Q8GM55" ], "nt": "ATGGCTAAAGAAATTTTACAAAATTCAGCACATGCATCAGTTCGCATGCAAAGAATTTCACCACGTAAAGCAAGATTAGTAGCAGATTTAATTAGATATAAATCAGCAACTCAAGCAATAGTTATTCTAAAACATACACACAAAAAAGCGTCAGAAATTATTCTAAAACTTCTAAATTCAGCAATTGCTAATGCAACAAATAATGCTGGACTTGATGCAACTAAACTTTATGTAACTACCATTTTAGTAAATGATGGACCTACACTAAAAAGATTCCAACCTCACTCACGTGGACGTGCTTATGCAATTTTAAAAAGAACAAGTCATTTCTTTATTGAATTAACAGAAATCAATAATGTTGAAGAAATCAACAAAAAAGGAGATAAATAA", "aa": "MAKEILQNSAHASVRMQRISPRKARLVADLIRYKSATQAIVILKHTHKKASEIILKLLNSAIANATNNAGLDATKLYVTTILVNDGPTLKRFQPHSRGRAYAILKRTSHFFIELTEINNVEEINKKGDK", "aa_hexdigest": "7a8b68311e501c927669bb50a65848bd", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC22A4", "ncbi_nrp_id": "WP_012855569.1", "uniref100_id": "UniRef100_Q8GM55", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A4", "RefSeq:WP_012855569.1", "UniRef:UniRef100_Q8GM55" ] }, "ips": { "uniref100_id": "UniRef100_Q8GM55", "uniref90_id": "UniRef90_Q8GM55" }, "psc": { "uniref90_id": "UniRef90_Q8GM55", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_Q98PY6", "cog_id": "COG0091", "cog_category": "J", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY6" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "AHCKFKLKGB_610", "locus": "GCA013367975_00596" }, { "type": "cds", "sequence": "contig_1", "start": 343343, "stop": 343447, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTTCATTAAAGAAAGCTCCCTTTGTTGATGATCATTTAATGAAAAAAGTTTTGGCTATTATTGAAAATAAATCACCAAAACGTCCAATTAAAACATGA", "aa": "MARSLKKAPFVDDHLMKKVLAIIENKSPKRPIKT", "aa_hexdigest": "5da938756da5c11a053df77e46912a7d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3904.734700000001, "isoelectric_point": 10.383809852600098 }, "id": "AHCKFKLKGB_611", "locus": "GCA013367975_00597" }, { "type": "cds", "sequence": "contig_1", "start": 343534, "stop": 344292, "strand": "-", "frame": 1, "gene": null, "product": "Large ribosomal subunit protein uL2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "nt": "ATGGCTATTAAAAAATTTAAAGCATATACCAATGGCCGTCGTAATATGTCTTCTCTTGATTATCAAGCAAATTTAACAGGACATGCTCCTGAAAAATCATTGCTTGTTGCTCTTCCAACAAAAGCAGGAAGAAACAATCAAGGTAAAATCACTACCCGTCATCAAGGTGGTAGATTGAAAAGATTCTATCGTATCATTGATTTTAAACGTCAAAAAGATGGAATCCCAGCTACTGTAAAAACTATTGAATATGACCCTAATCGTTCAGCTAATATTTCATTAGTTGTTTATAGAGATGGTGAAAAAAGATATATTTTAAGCCCAAAAGGTATCAAAATTGGACAAGTTATTTTGTCAGGTGATAATGTTGACATTCAAGTTGGTAACCATTTACCATTAGCCAACATTCCTGAAGGTACATTTGTTCACAATATTGAATTACAACCAAGACAAGGTGGAATCATTGCTAGATCAGCTGGATCATCAGCACAAATCTTAGGTAAAGATGAATCAGGTCGTTATGTAATTCTAAGATTGAAATCAGGCGAAGTTAGAAAAGTTTTAGCAATTAATCGTGCAACAGTTGGTGAAGTAGGAAACGAAGAACACTCACTTGTTAATATTGGTAAGGCAGGACGTAACCGCTTAAGAGGAATTAGACCAACAGTTCGTGGTTCAGCTATGAACCCTAATGATCACCCACATGGTGGTGGTGAAGGTAGACAACCTATTGGTAGAAAGAGTCCTATGACTCCATGA", "aa": "MAIKKFKAYTNGRRNMSSLDYQANLTGHAPEKSLLVALPTKAGRNNQGKITTRHQGGRLKRFYRIIDFKRQKDGIPATVKTIEYDPNRSANISLVVYRDGEKRYILSPKGIKIGQVILSGDNVDIQVGNHLPLANIPEGTFVHNIELQPRQGGIIARSAGSSAQILGKDESGRYVILRLKSGEVRKVLAINRATVGEVGNEEHSLVNIGKAGRNRLRGIRPTVRGSAMNPNDHPHGGGEGRQPIGRKSPMTP", "aa_hexdigest": "2d1053e9b4b069d6e507646decf318a5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3M0A1P8", "query_cov": 1.0, "subject_cov": 0.896797153024911, "identity": 0.897, "score": 457.0, "evalue": 1.04e-161, "valid": false, "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [], "id": "AHCKFKLKGB_612", "locus": "GCA013367975_00598" }, { "type": "cds", "sequence": "contig_1", "start": 344386, "stop": 344817, "strand": "-", "frame": 1, "gene": "rplW", "product": "50S ribosomal protein L23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_277872592.1", "SO:0001217", "UniParc:UPI002410E716", "UniRef:UniRef100_UPI002410E716", "UniRef:UniRef50_Q98PY3", "UniRef:UniRef90_D1J884" ], "nt": "ATGAATATAAATGAAGTTATTAAATACCCACTTTTAACAGAAAAAAGTGAATTATCACGTGAAAATAAAAACGTTTATACATTTGTAGTTGATAAAAGAACAAACAAAATCGAAGTTAAAAAAGCAGTTGAATTTATTTTTGATGTAAAAGTATTAAAAGTTAACATTACTAATTACGATAAAAAACCTGCTAAATTAGGACGTTTCTCAGGTTTTAAAAATTCAGTTAAAAAAGCAACTGTATATTTAGCAAAAGATTCAAAAATTATTCTATTTGCTGAAGAATTAAAGAAAGCTAACAAAGAAGCTGAAAAAGCAACAGCAGAAAAAGAAGTTAAAGAAGAAAAACCTGCTGAACCTACTGCCGCAGAATTAAAAGCAGCAGAAAAAATTAAAGCAGCTGAAACTAAAAAAGCTGCTGAAAACAAATAG", "aa": "MNINEVIKYPLLTEKSELSRENKNVYTFVVDKRTNKIEVKKAVEFIFDVKVLKVNITNYDKKPAKLGRFSGFKNSVKKATVYLAKDSKIILFAEELKKANKEAEKATAEKEVKEEKPAEPTAAELKAAEKIKAAETKKAAENK", "aa_hexdigest": "956c6ec80be3ad81bf415b8daf67b06e", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI002410E716", "ncbi_nrp_id": "WP_277872592.1", "uniref100_id": "UniRef100_UPI002410E716", "db_xrefs": [ "SO:0001217", "UniParc:UPI002410E716", "RefSeq:WP_277872592.1", "UniRef:UniRef100_UPI002410E716" ] }, "ips": { "uniref100_id": "UniRef100_UPI002410E716", "uniref90_id": "UniRef90_D1J884" }, "psc": { "uniref90_id": "UniRef90_D1J884", "gene": "rplW", "product": "50S ribosomal protein L23", "uniref50_id": "UniRef50_Q98PY3", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY3" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "AHCKFKLKGB_613", "locus": "GCA013367975_00599" }, { "type": "cds", "sequence": "contig_1", "start": 344817, "stop": 344954, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCTTATCAGCAAGAAACTTACCAAATGTAACTGTAACAAAAGTAACTAGTTTATCAGTTGAATCTTTATTGGCAGCAGATGTTTTAGTTATTAGTTCAGATGATGTTAAATACTTAGAAGGATTGGTTAAATAA", "aa": "MFLSARNLPNVTVTKVTSLSVESLLAADVLVISSDDVKYLEGLVK", "aa_hexdigest": "8430c512fd0c8253469c444377393a46", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4836.5984, "isoelectric_point": 4.8610639572143555 }, "id": "AHCKFKLKGB_614", "locus": "GCA013367975_00600" }, { "type": "cds", "sequence": "contig_1", "start": 345600, "stop": 345785, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314D514", "UniRef:UniRef90_UPI001314D514" ], "nt": "ATGGCAACAAAAAATGTAACATTAGAAAAATTCTACATTTCTGAAAAATTTGATGAAAATCGTAATATTTCATTTAATTTAAAAAAGGCTAACCAAAAATCATTCAAGAATTTTGAAAATTTTTCAGAAGCATTAACAGAATTTATTGAACTTTCTGAAAAATCAAAAAACAATACTAGAGTATGA", "aa": "MATKNVTLEKFYISEKFDENRNISFNLKKANQKSFKNFENFSEALTEFIELSEKSKNNTRV", "aa_hexdigest": "65cd46d9cd54cbf3847c176a252340c8", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 345488, "stop": 345785, "observations": { "insertion": [ 345521 ], "mutation": [ 345602 ], "stop": [ 345602 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314D514", "query_cov": 1.0, "subject_cov": 0.6224489795918368, "identity": 1.0, "score": 115.0, "evalue": 3.69e-32, "gene_start": 1, "gene_end": 61, "reference_start": 1, "reference_end": 61, "reference_sequence": "MATKNVTLEKFYISEKFDENRNISFNLKKANQKSFKNFENFSEALTEFIELSEKSKNNTRVWFHRDGAYRGSVGLEKAKLILEKVTGEKLKMKKQSTT" }, "paralog": false, "identity": 0.9897959183673469, "score": 184.0, "evalue": 4.84e-62, "description": "Internal stop codon at 345602. Frameshift due to insertion around 345521. Nonsense mutation around 345602.", "psc": { "uniref90_id": "UniRef90_UPI001314D514", "uniref50_id": "UniRef50_UPI001314D514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314D514", "UniRef:UniRef50_UPI001314D514" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314D514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314D514" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7242.053499999998, "isoelectric_point": 9.04905643463135 }, "id": "AHCKFKLKGB_615", "locus": "GCA013367975_00601" }, { "type": "cds", "sequence": "contig_1", "start": 345785, "stop": 346111, "strand": "-", "frame": 3, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_D1J886" ], "nt": "ATGCCAGGACATTTAGGAGCTGAACAAGTAACTGTTCAAAACCTAGAAGTTGTTAAAGAAGATGCAGAAAACAATATTTTATTGATCAAGGGATCAGTTCCTGGTGCTAAGGGAGCATTCTTAATCATTAAAACTGCTAAGAAATCAATTAAGAATAAACCAGTTATTAAATTATTGAATCTAAAAGAAGCTTTGGCTAAAAATGAAATCTTTACTCAAGCTAAAAAATACAATCTTGAATTAAACATGGATATGTCATTAAAAGAAATGAAAGCTGCTTTGGATGAAGCAATTGCTAAGGCCGGAGAAGAAGGAGAAAATAAATAA", "aa": "MPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK", "aa_hexdigest": "8579bc1a82bf19d1b9aee04b89886a80", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rplC" ], "truncated": "3-prime", "pseudogene": { "start": 345785, "stop": 346411, "observations": { "mutation": [ 346126, 346225 ], "stop": [ 346126, 346225 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J886", "query_cov": 1.0, "subject_cov": 0.40298507462686567, "identity": 1.0, "score": 201.0, "evalue": 3.99e-63, "gene_start": 1, "gene_end": 108, "reference_start": 161, "reference_end": 268, "reference_sequence": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVWKGMTMPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK" }, "paralog": false, "identity": 0.9903846153846154, "score": 400.0, "evalue": 7.48e-144, "description": "Internal stop codon at 346126, 346225. Nonsense mutation around 346126, 346225.", "psc": { "uniref90_id": "UniRef90_D1J886", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J886", "UniRef:UniRef50_Q98PY0", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "AHCKFKLKGB_616", "locus": "GCA013367975_00602" }, { "type": "cds", "sequence": "contig_1", "start": 346223, "stop": 346591, "strand": "-", "frame": 3, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_D1J886" ], "nt": "ATGAAAGGAATCTTAGGAAGAAAAGTTGGGATGACTCAACTATTTACTTCAGAAGGTAAATTAATACCTGTGACAGTAGTAGAAGTTAAACCAAATGTTGTTACTAAGGTTTTAACATCAGAAAAAAATGGTTATGTTGCTCTTCAATTAGCAACGGAAGATAAAAAATCTTCAAGAATCATTAAACCTGAAGTTAATTACTTTAAACAAGCAAATACAACACCTAAGCGCTTCGTAAAAGAAATCCGTGATATGTCAGGATTTTCATTAGGTGATACTGTAGATGCTTCATTATTTACCGCTGGAGAAGTTGTTGACGTAACAGCTATTTCTAAAGGTAAAGGATTTGCGGGTACCATCAAGAGATGA", "aa": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKR", "aa_hexdigest": "565ff623fc584ade78acfc33a3752bd4", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "rplC" ], "truncated": "5-prime", "pseudogene": { "start": 345920, "stop": 346591, "observations": { "mutation": [ 346126, 346225 ], "stop": [ 346126, 346225 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J886", "query_cov": 1.0, "subject_cov": 0.4552238805970149, "identity": 1.0, "score": 233.0, "evalue": 1.81e-75, "gene_start": 1, "gene_end": 122, "reference_start": 1, "reference_end": 122, "reference_sequence": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVWKGMTMPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK" }, "paralog": false, "identity": 0.9910313901345291, "score": 427.0, "evalue": 2.02e-154, "description": "Internal stop codon at 346126, 346225. Nonsense mutation around 346126, 346225.", "psc": { "uniref90_id": "UniRef90_D1J886", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J886", "UniRef:UniRef50_Q98PY0", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "AHCKFKLKGB_617", "locus": "GCA013367975_00603" }, { "type": "cds", "sequence": "contig_1", "start": 346661, "stop": 346969, "strand": "-", "frame": 3, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855575.1", "SO:0001217", "UniParc:UPI0001BC22A6", "UniRef:UniRef100_D1J887", "UniRef:UniRef50_D1J887", "UniRef:UniRef90_D1J887" ], "nt": "ATGAAACTAGAAGTCAAAATTAAATCTTTTGATGCCCACTTAGTAGATGAAGCTGCTAAAAAAATAGTTTTATTAGCTCAAGCTGAAAAAGCAACTATTAGTGGTCCAATTCCTTTACCAACACATAAAGAAATTATTACCATCTTAAGATCTGTTCACGTTAACAAGAAATCTCGTGAACAATTTGAAAGCAGAACTCATAAACGTTTAATCGTTTTAGATAAAGTTTCTGAATCATTAGTTGATAAAGCTAAGAGATTAGATTTATCTGCTGGTGTACAAATAAGTTTAAAAACTGTTAATCAATAA", "aa": "MKLEVKIKSFDAHLVDEAAKKIVLLAQAEKATISGPIPLPTHKEIITILRSVHVNKKSREQFESRTHKRLIVLDKVSESLVDKAKRLDLSAGVQISLKTVNQ", "aa_hexdigest": "f6c7d2f92638f7c729b3f6b0f0f4476d", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A6", "ncbi_nrp_id": "WP_012855575.1", "uniref100_id": "UniRef100_D1J887", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A6", "RefSeq:WP_012855575.1", "UniRef:UniRef100_D1J887" ] }, "ips": { "uniref100_id": "UniRef100_D1J887", "uniref90_id": "UniRef90_D1J887" }, "psc": { "uniref90_id": "UniRef90_D1J887", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_D1J887", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_D1J887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J887" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "AHCKFKLKGB_618", "locus": "GCA013367975_00604" }, { "type": "cds", "sequence": "contig_1", "start": 347514, "stop": 347621, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTGTATTTATATACAAATTCTTTGTCCAGATATGATTAAAAACAATTTAATGGGTAAAGAATCGGAAGGATATATCTATTTAGATCTTTCGGATGTTGATTAA", "aa": "MPCIYIQILCPDMIKNNLMGKESEGYIYLDLSDVD", "aa_hexdigest": "36f1d7a228e4ddf589d29e748b95b7bc", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4038.7023000000017, "isoelectric_point": 4.1011266708374015 }, "id": "AHCKFKLKGB_619", "locus": "GCA013367975_00605" }, { "type": "cds", "sequence": "contig_1", "start": 347670, "stop": 348314, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J888", "UniRef:UniRef90_D1J888" ], "nt": "ATGAAAAAAAATATTTTATTAAGTTTTATTTTTCCGGCTGTGTCATTATTACCTCTTTTAAGCATATCTTGCAAGGAAACTACCACAATTAATAATACCTTAGATACAAGTAATAACACTATTGATATAGATAATAGTGGCACTACAGATACTAATAACAAAAATACAGATAGTACAAATACTGGTACCACTAATACTGATAATAAGAATATAGGTAATTCTAGTGTTGGCACTACAGATACTAATAACAAAAATACAGACAGCACAAATACCGGTACCGCCGATACTAACGACAAGAAAGATGACAATAAAGATACAAATAACGAAAATACTGATGATACAGATCCCGTACAACCCGATAGACTTCATAGAATGTTTCAAAGAAAGCTATTATCCGCTTCTCAAATTTCAGAAATAACAACACAATTTTCATTTGAACTTTCCAAAGAAGGCAAAAAATTTGTAGCCAATAATGGAGCCAAAGCCTTAATTGAAATTGTCAATAAAGCAATTGATGAAACTAAAAAATACGATACTATTGAAATGTTAAAAAGTCAAGAAAGAATTGCTTATACATATTTTATGGAAGCTAATGACATTAAAAAATATTTTGATATAAAAATTCCTGAAAATCTTATGCCTTGA", "aa": "MKKNILLSFIFPAVSLLPLLSISCKETTTINNTLDTSNNTIDIDNSGTTDTNNKNTDSTNTGTTNTDNKNIGNSSVGTTDTNNKNTDSTNTGTADTNDKKDDNKDTNNENTDDTDPVQPDRLHRMFQRKLLSASQISEITTQFSFELSKEGKKFVANNGAKALIEIVNKAIDETKKYDTIEMLKSQERIAYTYFMEANDIKKYFDIKIPENLMP", "aa_hexdigest": "712b03fc543f53c51cf15931dd771fbf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 347514, "stop": 348308, "observations": { "mutation": [ 347672 ], "stop": [ 347672 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J888", "query_cov": 0.9906542056074766, "subject_cov": 0.7881040892193308, "identity": 0.934, "score": 246.0, "evalue": 3.46e-79, "gene_start": 3, "gene_end": 214, "reference_start": 6, "reference_end": 217, "reference_sequence": "MKGIWKNILLSFIFPAVSLLPLLSISCKETTTINNTLDTNNNTIDIDNSGTTDTNDKNTNSTNTGTTDTDNKNIGNSSVDTTDTNDKNTDSTNTGTTDTSDKKDDNKDTNNENTDDTDPEQPNRLHRIFQRKLLSASQISEITTQFSFELSKEGKKFVANKGAKALIEIVNKAIDETKKYDTIEMLKSQERIAYTYFMEANDIKKYFDIKIPENLMPWGHKLLIEFLKKGEEKNMPCIYIQILCPDMIKNNLMGKESEGYIYLDLSDVD" }, "paralog": false, "identity": 0.9431818181818182, "score": 360.0, "evalue": 1.66e-126, "description": "Internal stop codon at 347672. Nonsense mutation around 347672.", "psc": { "uniref90_id": "UniRef90_D1J888", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J888", "UniRef:UniRef50_D1J888" ] }, "pscc": { "uniref50_id": "UniRef50_D1J888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J888" ], "product": "Variable surface lipoprotein" } }, "id": "AHCKFKLKGB_620", "locus": "GCA013367975_00606" }, { "type": "cds", "sequence": "contig_1", "start": 348748, "stop": 349065, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATATTGAAGGCCCAGGACATGGTGGACAAGTTATGATTTCAAACTCTTATCTTGATGGATCTTATTCAGAAATATATCCTGAAATATCTCAAGATGAAGCTGGACTTGCAAAAATGTTCAAAAGATTCTCATTCCCTGGTGGAACTGCAAGCCATGCAGCACCCGAAACACCCGGATCAATTCATGAAGGTGGTGAATTAGGATATTCTATAAGCCATGGAACAGGAGCAATATTAGATAACCCTGATGTTATTTGTGCAGCTGTTGTTGGCGATGGTGAAGCAGAAACAGGCCCACTAGCAACTAGCTGA", "aa": "MFYIEGPGHGGQVMISNSYLDGSYSEIYPEISQDEAGLAKMFKRFSFPGGTASHAAPETPGSIHEGGELGYSISHGTGAILDNPDVICAAVVGDGEAETGPLATS", "aa_hexdigest": "cb9a70a1207895298182aa6f7a6c75c0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10793.721599999992, "isoelectric_point": 4.3166601181030275 }, "id": "AHCKFKLKGB_621", "locus": "GCA013367975_00607" }, { "type": "cds", "sequence": "contig_1", "start": 348936, "stop": 349256, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAGATTACTTTTATCTTCGCTTCATTCAACGAAAATTGGATTTCAGCCTAAACCTTCAAAGTATTTTTTAATTTCTTCTTTTGGTTTTCTTGAAAGAAGTGTAGGGTTTGAAATTTTTCCACCATTCAAATGTAATATAGGAAGTATTGCCCCATCATTTACAGGGTTAATAAAAGCATTTGAGAATCAGCTAGTTGCTAGTGGGCCTGTTTCTGCTTCACCATCGCCAACAACAGCTGCACAAATAACATCAGGGTTATCTAATATTGCTCCTGTTCCATGGCTTATAGAATATCCTAATTCACCACCTTCATGA", "aa": "MSRLLLSSLHSTKIGFQPKPSKYFLISSFGFLERSVGFEIFPPFKCNIGSIAPSFTGLIKAFENQLVASGPVSASPSPTTAAQITSGLSNIAPVPWLIEYPNSPPS", "aa_hexdigest": "e74dad0e4e0293078566f4399dcc17f9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11315.932199999985, "isoelectric_point": 9.359794044494631 }, "id": "AHCKFKLKGB_622", "locus": "GCA013367975_00608" }, { "type": "cds", "sequence": "contig_1", "start": 349267, "stop": 349362, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAATCATTAGATAAGGCTATTGAATCAATTAAAGAAATTCAAGCAGAAGCAAGAAAAAAACCTGCTGAAGAAGCAACAAGACCAACTTGA", "aa": "MAKSLDKAIESIKEIQAEARKKPAEEATRPT", "aa_hexdigest": "9dabdcbb4dcd8d8b3e81f6139aafeee5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3440.9214000000006, "isoelectric_point": 8.141083717346195 }, "id": "AHCKFKLKGB_623", "locus": "GCA013367975_00609" }, { "type": "cds", "sequence": "contig_1", "start": 349714, "stop": 349842, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAAATGGCAAAATTTGCTGCTGATATAATGAAAGATAACCCTTCATCATTTAGAGTATTTGGACCTGATGAAACTAAATCAAATCGTATGTTTGCATTATTCAATGTAACAAATAGACAATGA", "aa": "MAEMAKFAADIMKDNPSSFRVFGPDETKSNRMFALFNVTNRQ", "aa_hexdigest": "7538684bd81affb55bcf87471adf19fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4814.4602, "isoelectric_point": 8.253452110290528 }, "id": "AHCKFKLKGB_624", "locus": "GCA013367975_00610" }, { "type": "cds", "sequence": "contig_1", "start": 350230, "stop": 350358, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAGCCTTAACCGAAAGAAATGTAATTAACTTAATTGTTGCTTCAAAACAACCAAGAGAACAATTCTTTACAGTTGAAGATGCTGAAGAATTACTAGAAAAAGGTTATAAAGTTGTTCCTTGA", "aa": "MNKALTERNVINLIVASKQPREQFFTVEDAEELLEKGYKVVP", "aa_hexdigest": "06265444dd5f8e3c2b7c8e8889e02782", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4848.5314, "isoelectric_point": 5.189195442199705 }, "id": "AHCKFKLKGB_625", "locus": "GCA013367975_00611" }, { "type": "cds", "sequence": "contig_1", "start": 350434, "stop": 350874, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGCTATTTCACTAATTAACCAAGAATATCCACATCTAAAAATAAGATATGTTTATGTTCTTGACTTATTAAAATTGAGATCTAGAAAAATAGATCCAAGAGGAATTAGCGATGAAGAATTTGATAAGGTATTTACTAAAAATAAACCTATCATATTTGCTTTCCATGGATTTGAAGGATTATTAAGAGACATCTTCTTTACAAGATCTAACCACAATTTAATTGCACACGGATATCGTGAAAACGGTGATATTACAACATCATTCGATATTAGACAATTAAGTGAAATGGATAGATATCATATTGCTAAAGATGCAGCAGAAGCAGTATATGGAAAAGATGCAAAAGCATTTATGAATAAATTAGACCAAAAACTAGAATATCATAGAAACTACATTGATGAATATGGATATGATATGCCAGAAGTTGTTGAATGA", "aa": "MAAISLINQEYPHLKIRYVYVLDLLKLRSRKIDPRGISDEEFDKVFTKNKPIIFAFHGFEGLLRDIFFTRSNHNLIAHGYRENGDITTSFDIRQLSEMDRYHIAKDAAEAVYGKDAKAFMNKLDQKLEYHRNYIDEYGYDMPEVVE", "aa_hexdigest": "59f58ddc36c55ba5c33ce12997ad2aed", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA37XNB4", "query_cov": 0.9863013698630136, "subject_cov": 0.8674698795180723, "identity": 0.59, "score": 189.0, "evalue": 3.88e-59, "valid": false, "product": "Xylulose 5-phosphate/Fructose 6-phosphate phosphoketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A3B0NYC0" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17242.383899999986, "isoelectric_point": 6.071961784362792 }, "id": "AHCKFKLKGB_626", "locus": "GCA013367975_00612" }, { "type": "cds", "sequence": "contig_1", "start": 350981, "stop": 351196, "strand": "+", "frame": 2, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855578.1", "SO:0001217", "UniParc:UPI0001BC22A9", "UniRef:UniRef100_D1J890", "UniRef:UniRef50_Q6KIG2", "UniRef:UniRef90_D1J890" ], "nt": "ATGCAAAAAGATATTCACCCAAAATACAACCAAATTAAAGCTGTTTGCTCATCATGCAATTCAACAATAGAATTTGGTTCAACTGCTGATAAAGTTACTTTAGATGTTTGCTCTAATTGTCATGCTTTCTACACAGGAAACCGTACAGATGTTAAAGCTCGTGGACGTGTTGAAAGATTTAATAAGATATTAGAAAAATCACATAAAAAGAACTAA", "aa": "MQKDIHPKYNQIKAVCSSCNSTIEFGSTADKVTLDVCSNCHAFYTGNRTDVKARGRVERFNKILEKSHKKN", "aa_hexdigest": "14ec0f3da0d8b604325a5ed87f9f7f2a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A9", "ncbi_nrp_id": "WP_012855578.1", "uniref100_id": "UniRef100_D1J890", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A9", "RefSeq:WP_012855578.1", "UniRef:UniRef100_D1J890" ] }, "ips": { "uniref100_id": "UniRef100_D1J890", "uniref90_id": "UniRef90_D1J890" }, "psc": { "uniref90_id": "UniRef90_D1J890", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_Q6KIG2", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG2" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "AHCKFKLKGB_627", "locus": "GCA013367975_00613" }, { "type": "cds", "sequence": "contig_1", "start": 351291, "stop": 351530, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J891", "UniRef:UniRef90_D1J891" ], "nt": "ATGGAATATTTGTTATATTTGAAATTGAAGGGAGAAATAAAAATGAATATAAATTTCAAACTAATGAGCGAAGAAGAAACAAAAAATATTGTAGGAGGTTTTGCAATTACTGCTTTAATTTCAACTATTTTGTCTGTAGTAACAAGCACTGTTGGAATTATTGGAACTTCAATAGGCTTGGCTAAGACTTTAAATGCAAATAAAGGAGAAGTAAAAGCCGGGAATATTTCATTGAAATGA", "aa": "MEYLLYLKLKGEIKMNINFKLMSEEETKNIVGGFAITALISTILSVVTSTVGIIGTSIGLAKTLNANKGEVKAGNISLK", "aa_hexdigest": "5ae79da8f2d0aaa4e27df05c8fffdaa5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J891", "query_cov": 1.0, "subject_cov": 0.8144329896907216, "identity": 0.987, "score": 140.0, "evalue": 4.64e-42, "valid": true, "uniref50_id": "UniRef50_D1J891" }, "pscc": { "uniref50_id": "UniRef50_D1J891", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J891" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8445.945599999995, "isoelectric_point": 9.347996330261232 }, "id": "AHCKFKLKGB_628", "locus": "GCA013367975_00614" }, { "type": "cds", "sequence": "contig_1", "start": 352948, "stop": 353364, "strand": "-", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE0DEE4", "UniRef:UniRef90_UPI002FE0DEE4" ], "nt": "ATGCTTGGTGGAATTAGTGACTTTGATTTTAAAGAAATTACGCCAACTTCAATTCATGCTTTAATGTTAGAAAAATTGCATAATCTGGAAGCAAATATATTATTGAATTACGAACAATATAGATTTGTCAATGTTATTAAAGATATTAATAATTTTGTTATTGATTTAAGTAGTTACTATATTTCTATAACCAAAGATATTTTATATCTTGAAAAACAAGATGACCCTGAACGTAGAATGGTTCAATATGTTTTTAGTAACATTATTGAGGTCTTATTAAAAGCTCTTGCCCCAATATTGCCAACTACAACCGAAGAAATTTATAAATATTACAATAAACCTAACAAGTTGCCATCTGTTCATCTATTGCCATTTTTAGAAAGAAAAGATTTCGATTTTAAATTACAAGATCAATGA", "aa": "MLGGISDFDFKEITPTSIHALMLEKLHNLEANILLNYEQYRFVNVIKDINNFVIDLSSYYISITKDILYLEKQDDPERRMVQYVFSNIIEVLLKALAPILPTTTEEIYKYYNKPNKLPSVHLLPFLERKDFDFKLQDQ", "aa_hexdigest": "e55a56ea383829b50658805c31a39248", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 352840, "stop": 353355, "observations": { "mutation": [ 352860, 352950 ], "stop": [ 352860, 352950 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE0DEE4", "query_cov": 0.9782608695652174, "subject_cov": 0.7627118644067796, "identity": 0.985, "score": 266.0, "evalue": 2.01e-89, "gene_start": 4, "gene_end": 138, "reference_start": 3, "reference_end": 137, "reference_sequence": "MLGISDFDFKEITPTSIHALMLEKLHNLEANILLNYEQYRFVNVIKDINNFVIDLSSYYISITKDILYLEKQDDPERRMVQYVFSNIIEVLLKALAPILPTTTEEIYKYYNKPNKLPSVHLLSFLERKDFDFKFQDQWKEFFEIKDEVYKLIEEKIKSQAIKRPNEAWVVIKAIQTL" }, "paralog": false, "identity": 0.9707602339181286, "score": 326.0, "evalue": 1.01e-115, "description": "Internal stop codon at 352860, 352950. Nonsense mutation around 352860, 352950.", "psc": { "uniref90_id": "UniRef90_UPI002FE0DEE4", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI002FE0DEE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE0DEE4", "UniRef:UniRef50_UPI002FE0DEE4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE0DEE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE0DEE4" ], "product": "class I tRNA ligase family protein" } }, "id": "AHCKFKLKGB_629", "locus": "GCA013367975_00615" }, { "type": "cds", "sequence": "contig_1", "start": 354112, "stop": 354390, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGCCAGTTGTTTTTGGTCATCATGTTAGTTTAGATACTGGTACTGGCTTAGTTCACATTGCTCCATTATTTGGTGAAGATGACTTTATTATCGGCAAAAAAGAAAACCTAGAAATGATTATGCACATAAACGATGATGGAACAATCAATGATAAAGGCTTTGAATATAGCGGTGTTTTTTACGATGATGCAAATCCTTTAATTGGCAAATTTTTAGCAGAAAATAATCAATTAATTGCGTTTAAGAAAATTAAACACTCATATCCTCACGATTGA", "aa": "MGPVVFGHHVSLDTGTGLVHIAPLFGEDDFIIGKKENLEMIMHINDDGTINDKGFEYSGVFYDDANPLIGKFLAENNQLIAFKKIKHSYPHD", "aa_hexdigest": "ec0cfcc482567d5921c86253b942139e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10259.48839999999, "isoelectric_point": 5.131447029113769 }, "id": "AHCKFKLKGB_630", "locus": "GCA013367975_00616" }, { "type": "cds", "sequence": "contig_1", "start": 354469, "stop": 354591, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGGCTAACTCTGGAGTTGCAATTAACGATCAATTCGAATATGCAAAAGTGTTAGTAAATGACAATAAATATATAATTGCAAAAGATTTAGTTGAATCAGTTGCTGCAGTTGCTAAATGA", "aa": "MLANSGVAINDQFEYAKVLVNDNKYIIAKDLVESVAAVAK", "aa_hexdigest": "dae79f8ebe2348c548447731b026d859", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4325.9333000000015, "isoelectric_point": 4.8610639572143555 }, "id": "AHCKFKLKGB_631", "locus": "GCA013367975_00617" }, { "type": "cds", "sequence": "contig_1", "start": 354745, "stop": 354990, "strand": "-", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AA8A25", "UniRef:UniRef90_UPI0023AA8A25" ], "nt": "ATGTTGCTAGAAGCTAAAAAGAATGCTAAAGATTTTAGTGTTATTGAATTAAGAAAAAAGGCAGCAGAATATGCTTTAAGTCAAGTTGAACATCAAAAGGCTCAATTTAAAAAATTAAGTTTATTGACAGACTTTTCAGAAATTTATGTGACATTGGATAAAAACTTAGAATTTAAACAATTAGAACTTTTTGCTAAAATGGTTAAAGAAGGCTTAATTTATCGTGATTTAAAACCAGTTTATTGA", "aa": "MLLEAKKNAKDFSVIELRKKAAEYALSQVEHQKAQFKKLSLLTDFSEIYVTLDKNLEFKQLELFAKMVKEGLIYRDLKPVY", "aa_hexdigest": "60f04ddce680b0da4157d568877814b2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 354442, "stop": 355008, "observations": { "mutation": [ 354471, 354597, 354612, 354747 ], "stop": [ 354471, 354597, 354612, 354747 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AA8A25", "query_cov": 1.0, "subject_cov": 0.4175257731958763, "identity": 0.988, "score": 153.0, "evalue": 9.15e-46, "gene_start": 1, "gene_end": 81, "reference_start": 7, "reference_end": 87, "reference_sequence": "MPIEHKMLLEAKKNAKDFSVIELRKKAAEYALSQVEHQKAQFKKLSLFTDFSEIYVTLDKNLEFKQLELFAKMVKEGLIYRDLKPVYWSPSSQSALAEAEVEYADHVSPSLYVAFKVIEGNKYVENGDYLIIWTTTPWTLLANSGVAINDQFEYAKVLVNGNKYIIAKDLVESVAAVAKWEEYQVLSTFKGKEF" }, "paralog": false, "identity": 0.9468085106382979, "score": 338.0, "evalue": 3.03e-121, "description": "Internal stop codon at 354471, 354597, 354612, 354747. Nonsense mutation around 354471, 354597, 354612, 354747.", "psc": { "uniref90_id": "UniRef90_UPI0023AA8A25", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI0023AA8A25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AA8A25", "UniRef:UniRef50_UPI0023AA8A25" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AA8A25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AA8A25" ], "product": "class I tRNA ligase family protein" } }, "id": "AHCKFKLKGB_632", "locus": "GCA013367975_00618" }, { "type": "cds", "sequence": "contig_1", "start": 355021, "stop": 355314, "strand": "-", "frame": 1, "gene": null, "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352TTR4" ], "nt": "ATGGAAGAAAAGAAAGATTATAAAGACACGTTATTGATGCCAAATACTGATTTTCCAATGAAAGCAAATTTAGTTGAAAAAGAATTGGAATATCAAAAACAATGGCTAGATATTAAGTTGTATCAAACTATTTTAAATAGAAATAAGAACAGTCAATCATTTGTATTACATGACGGTCCGCCATATGCAAATGGCAATATTCATGTTGGACACGCTCTAAACAAAATTCTAAAAGATATAATTGTAAGATTTAGAAGTATGCAGGGATATTATTCTCCATATGTTCCGGGTTGA", "aa": "MEEKKDYKDTLLMPNTDFPMKANLVEKELEYQKQWLDIKLYQTILNRNKNSQSFVLHDGPPYANGNIHVGHALNKILKDIIVRFRSMQGYYSPYVPG", "aa_hexdigest": "68b231c91ac2e4767637ea05b37ce65a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00190B9126", "query_cov": 0.9381443298969072, "subject_cov": 0.9891304347826086, "identity": 0.626, "score": 129.0, "evalue": 2.2e-37, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A352TTR4" }, "pscc": { "uniref50_id": "UniRef50_A0A352TTR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352TTR4" ], "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_633", "locus": "GCA013367975_00619" }, { "type": "cds", "sequence": "contig_1", "start": 355558, "stop": 355794, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "TTGATAAGCAACATTAATAAATTGATTTTAGAAGTTAGCGAAAATATTGAAAAAACTAAGTTTAATATTGCAATAAGCAAAATGATGGTCTTTACTAATTACTTAACATCTTTAAAAACTATTCCATCATCATTTGTTTTAAAACAATTTGCTATTTTATTAAGTCCATTTGCGCCATTTTTAGCTGAAGAACTTTTGTTTATGTTGAATGAAAAATCAATTCAATATCAAACTTGA", "aa": "MISNINKLILEVSENIEKTKFNIAISKMMVFTNYLTSLKTIPSSFVLKQFAILLSPFAPFLAEELLFMLNEKSIQYQT", "aa_hexdigest": "dcb4ca15054ee8dfc8bc6d4d2fe25e27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 95, "aa_cov": 0.9358974358974359, "hmm_cov": 1.0, "evalue": 5.612995714916336e-13, "score": 44.33252716064453, "start": 1, "stop": 73 } ], "seq_stats": { "molecular_weight": 8978.595699999998, "isoelectric_point": 8.008859062194826 }, "id": "AHCKFKLKGB_634", "locus": "GCA013367975_00620" }, { "type": "cds", "sequence": "contig_1", "start": 355900, "stop": 356094, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGGTATTGTTAATACAAAAGAACCATTTACAAAGCTAATAAACCAAGGATTAATTATGGGACCTGATGGTCAAAAAATGTCTAAATCTCTAGGTAATGTTATAAATCCTGATGATTTTATTTTATCTCATGGAGCGGATAGTTTAAGAGTTTATGAAATGTTTATGGGGCCTTTGGTAGAATCAAAATCTTGA", "aa": "MGIVNTKEPFTKLINQGLIMGPDGQKMSKSLGNVINPDDFILSHGADSLRVYEMFMGPLVESKS", "aa_hexdigest": "7700a8b03e05cbedaece89c5a097eac2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 48, "aa_cov": 0.75, "hmm_cov": 1.0, "evalue": 6.643571061395789e-16, "score": 53.310489654541016, "start": 5, "stop": 52 } ], "seq_stats": { "molecular_weight": 6998.0638, "isoelectric_point": 5.587352561950683 }, "id": "AHCKFKLKGB_635", "locus": "GCA013367975_00621" }, { "type": "cds", "sequence": "contig_1", "start": 356503, "stop": 356757, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "ATGTCATATGGAAGTGGAGCAGTAATGTGTGTGCCCGGCCATGATCAAAGAGATTATGAATTTGCTAAAAAATTTGATTTAAAAATTAAAAAAATTATTGAATGTGATGAATTACCATTTTTAGGAGATGGTATTCATATCAATTCAGATATTGCAAATGGTTTAGATAATGCAGGGGCAGTAAAATGTATTTTTAATGAATTGAAAGCAAATAATTTGGCAGATGAAAAAGTAAGTTATAAATTACGCGATTGA", "aa": "MSYGSGAVMCVPGHDQRDYEFAKKFDLKIKKIIECDELPFLGDGIHINSDIANGLDNAGAVKCIFNELKANNLADEKVSYKLRD", "aa_hexdigest": "feb5be2e345d142cf793d6cf1fd3e4d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 77, "aa_cov": 0.8095238095238095, "hmm_cov": 1.0, "evalue": 4.977646883761176e-19, "score": 63.81592559814453, "start": 1, "stop": 68 } ], "seq_stats": { "molecular_weight": 9322.527299999992, "isoelectric_point": 5.265132331848144 }, "id": "AHCKFKLKGB_636", "locus": "GCA013367975_00622" }, { "type": "cds", "sequence": "contig_1", "start": 356776, "stop": 357024, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "TTGCAACTCTCAACATTTACTACTAGATTGGATACTATCTATGGTGTAACTGCAATAGTAATTTCTCCCGAACACCCACTTCTTCAAAAAATAACTACCGCAAAATATCATGATGATATTTCTAACTATTTAAAAGAAGTTAAATTAAAAAATGAATTGGAAAGAACATCTTTAAATAAAGACAAAACAGGATATTTTACTGGCTCGTATGCAATTCATCCATTGACTAATGAATTAATTCCTATTTGA", "aa": "MQLSTFTTRLDTIYGVTAIVISPEHPLLQKITTAKYHDDISNYLKEVKLKNELERTSLNKDKTGYFTGSYAIHPLTNELIPI", "aa_hexdigest": "191f04c27562310af5aaf71b65e975e4", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 81, "aa_cov": 0.9878048780487805, "hmm_cov": 0.9753086419753086, "evalue": 2.784573970147506e-24, "score": 80.93769073486328, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 9353.616299999994, "isoelectric_point": 6.8211565017700195 }, "id": "AHCKFKLKGB_637", "locus": "GCA013367975_00623" }, { "type": "cds", "sequence": "contig_1", "start": 357526, "stop": 357648, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCTTACCCTTCGGGAATGGGATTGCACGTTGGGCATTTAGAGGGTTATACCGCTACTGATATTATTGCTAGGTATAAACGCCTAAATGGTTTTGATGTGTTGCATCCTATTGGCTGA", "aa": "MFPYPSGMGLHVGHLEGYTATDIIARYKRLNGFDVLHPIG", "aa_hexdigest": "21af7fc6286f9ee63374d2bcb75ef168", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4445.087, "isoelectric_point": 6.810016059875489 }, "id": "AHCKFKLKGB_638", "locus": "GCA013367975_00624" }, { "type": "cds", "sequence": "contig_1", "start": 358030, "stop": 359256, "strand": "+", "frame": 1, "gene": null, "product": "Lmp protein", "db_xrefs": [ "RefSeq:WP_175933482.1", "SO:0001217", "UniParc:UPI00158D8469", "UniRef:UniRef100_UPI00158D8469", "UniRef:UniRef50_A0A454CAH5", "UniRef:UniRef90_A0A454CAH5" ], "nt": "ATGAAAAGAAAAATTTTACTATTTACCCCCTTAATTATTGCTGGTAGCTTAATACCAACAACATTGACAGCATGCAATAATGAAGATAGCAAACTTACAAAGCAATTAAAAGCAAAGCAAACATTTAATAATTTAAAGAATAAATTGCAAGATTTATTAAACTCAGAAGATGCAAAAGATATTGATGCAAATAAAGAAATTAATACACTTGCAAACACAACAATTGATGATAATTCTCAAATACAAGACATTGAAAATAAAATCAAAACTATTGAAGATGCTATTGAATCATTGACTAAGAAAATTAATGATAAAAAAATCCTAATAAAACTAAGACAAGATTTCAACAAAGCAAAAGAAGATCTAAGAAATATTTTGACATCAAAAGAAGTGGCGGATATTGAAAAAGAAATCGAATTTAACACTCTAAATACTACTGATATTGCCAAAGATTCTTTGCAACAAGAAATTCAAGCCAAAATACAAAAAATTCAAGAAGCTATACAATCAATAAACAAAAAAATTAGCGACAAAAAGCAATTAAACAAAGAACAACAAAATCTTGTTGAAGCAAAAAATACACTTGAAAATCTTATTGCTACAGAAGATGCAAAAACAGTTGGAATTGGTGAAGCAAAAAAAATTCTTGATGATAATAAGAATAATAAAATATCAACTATTGAAGAAATTAAAAAAGCAATAAATGATTTGAATGATATTACAAACAAATTAAATCAAAAAATTGCAAACAAAAAGCAAGAATTATTAGAAAATTTCAAAAAATCTAAACAAGATTTAGAAGAATACATAAATAAAAATTTAAATGAAATCAGATTTATTGAAGTTAAAGAAAAAGCTCAAAAAACCATTGAAAAATTTTCTAATATAAACAAAGATAATTCCTCAATCAAAGAAATTACAGATGCTACTATCTCACTAAATAAAGCTAAAGAAGTTGCTTTGAAAGAAGAGGAAGAAATCAAAAAATTAAACGAAGTCTTGAAATATGTCAATAAAAGAAAAGAACTAGAAGAATTAATAAAATCATCAAATATTCAATCAAATAATGATGCTATTAAAAAAGCTCAAAAAACTTTAGACGATAATAAAATAGCAAATACCAACGAATCATCTTCAAAAGATTTTGAAGAAAAAAGAAAAAATATTGAAAAAGCAATAATTGAACTAAAGGCAGCAATAGAAGCAACAAAAAAATCTAAGAATTAA", "aa": "MKRKILLFTPLIIAGSLIPTTLTACNNEDSKLTKQLKAKQTFNNLKNKLQDLLNSEDAKDIDANKEINTLANTTIDDNSQIQDIENKIKTIEDAIESLTKKINDKKILIKLRQDFNKAKEDLRNILTSKEVADIEKEIEFNTLNTTDIAKDSLQQEIQAKIQKIQEAIQSINKKISDKKQLNKEQQNLVEAKNTLENLIATEDAKTVGIGEAKKILDDNKNNKISTIEEIKKAINDLNDITNKLNQKIANKKQELLENFKKSKQDLEEYINKNLNEIRFIEVKEKAQKTIEKFSNINKDNSSIKEITDATISLNKAKEVALKEEEEIKKLNEVLKYVNKRKELEELIKSSNIQSNNDAIKKAQKTLDDNKIANTNESSSKDFEEKRKNIEKAIIELKAAIEATKKSKN", "aa_hexdigest": "4fc3c0b44b37642f8d228e56f6702967", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00158D8469", "ncbi_nrp_id": "WP_175933482.1", "uniref100_id": "UniRef100_UPI00158D8469", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D8469", "RefSeq:WP_175933482.1", "UniRef:UniRef100_UPI00158D8469" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D8469", "uniref90_id": "UniRef90_A0A454CAH5" }, "psc": { "uniref90_id": "UniRef90_A0A454CAH5", "product": "Lmp protein", "uniref50_id": "UniRef50_A0A454CAH5" }, "pscc": { "uniref50_id": "UniRef50_A0A454CAH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAH5" ], "product": "Lmp protein" }, "genes": [], "id": "AHCKFKLKGB_639", "locus": "GCA013367975_00625" }, { "type": "cds", "sequence": "contig_1", "start": 359596, "stop": 360084, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D52A", "UniRef:UniRef90_UPI002410D52A" ], "nt": "ATGGGTAAAAAAGCAAAATTATTTGTCTTTGGTGGGATTAGTTTTTTAGCAGGAGCTTCAATTATTGGTTCTGTTGCAGCATATGCCTCAATTAATAAAAAGAATGAAATTAGAGTTAGCGAATATAATAAATTTCTTGAAGAAAAAACTAATTTAAATTCTTTTAGCAATTTAGAAAATGATAAAAAAATTAGTTCAATAAAATCTAATACATTAAGTGAAGTTGAAAAGATAGATAAAAATTCAGATTTATTTACTATTCAAAAAGCCCGCAAAATAGTCAACGAATCCATTGAAAATATCAAAAATATAATAGCATCGCATAAAAAAATGTTATTAGAAAAAGAAAAAGCGTTTATTCAATATAACAATGTAAAAAAGCAATTGCAAGATATGCTTTTAAAAATTTTTGACTTTTTAAATAAAGAAAGCCAAGAACATTATTACATTGGCGATGGAAACGAAAAAATAATAAACAAACAAAATTGA", "aa": "MGKKAKLFVFGGISFLAGASIIGSVAAYASINKKNEIRVSEYNKFLEEKTNLNSFSNLENDKKISSIKSNTLSEVEKIDKNSDLFTIQKARKIVNESIENIKNIIASHKKMLLEKEKAFIQYNNVKKQLQDMLLKIFDFLNKESQEHYYIGDGNEKIINKQN", "aa_hexdigest": "c39ee29bd425da6267b975f5c4819be0", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 359596, "stop": 360250, "observations": { "insertion": [ 360205 ], "mutation": [ 360082 ], "stop": [ 360082 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410D52A", "query_cov": 1.0, "subject_cov": 0.7465437788018433, "identity": 0.981, "score": 288.0, "evalue": 4.34e-97, "gene_start": 1, "gene_end": 162, "reference_start": 1, "reference_end": 162, "reference_sequence": "MGKKAKLFVFGGISFLVAASIIGSVAAYASINKKNEIRVSEYNKFLEEKTNLNSFSNLENDKKISSIKSNTLSEVEKIDKNSDLFTIQKARKIVNESIENIKNIIASHKKMLLEKEKAFIQYNNVKKQLQDMLLKIFDFLNKESQGHYYIGDGNEKIINKQNWLLKEINENQKYFSNITIDSDIDSIKLATTKLVEIKFLINENKKHINLPKNITAY" }, "paralog": false, "identity": 0.967741935483871, "score": 400.0, "evalue": 9.28e-144, "description": "Internal stop codon at 360082. Frameshift due to insertion around 360205. Nonsense mutation around 360082.", "psc": { "uniref90_id": "UniRef90_UPI002410D52A", "uniref50_id": "UniRef50_UPI002410D52A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410D52A", "UniRef:UniRef50_UPI002410D52A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410D52A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D52A" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18528.093999999994, "isoelectric_point": 9.397959327697755 }, "id": "AHCKFKLKGB_640", "locus": "GCA013367975_00626" }, { "type": "cds", "sequence": "contig_1", "start": 360427, "stop": 360555, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGACAAAACTGCACTTCTTGAGGAATTCGACATTGATTTTTTTGGATTCAAAATCAAAGATAAAAATTTCTATAAAAATTATGTTGAGTTGTTGACACCATATAAAAAGGCTATTGATTTGTGA", "aa": "MYDKTALLEEFDIDFFGFKIKDKNFYKNYVELLTPYKKAIDL", "aa_hexdigest": "06aa18affaf7abae4b9665d86555c85e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5128.885300000001, "isoelectric_point": 5.086430549621581 }, "id": "AHCKFKLKGB_641", "locus": "GCA013367975_00627" }, { "type": "cds", "sequence": "contig_1", "start": 360619, "stop": 360849, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024112F2E", "UniRef:UniRef90_UPI0024112F2E" ], "nt": "TTGTATATTAATGGTTATACTATCTTTTTTAAACCTAAAAAAATATATGCTTTGCAAAATCAACCTGAACTAGACAAAGAACTAACTGAAACTACTGATGATTATAAATTTAAACTAATGCTTGTTCCAAAAGAAAATACTATAGAACATAATAAATTAATAGAAAATTTTAAAAACTATTGCGACGATTTAATGAATAGAAGCAAAAAATTAAAAAACGACAATCAATAA", "aa": "MYINGYTIFFKPKKIYALQNQPELDKELTETTDDYKFKLMLVPKENTIEHNKLIENFKNYCDDLMNRSKKLKNDNQ", "aa_hexdigest": "df804558586bfb37bb00d21a1bf88a3f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 360523, "stop": 360849, "observations": { "mutation": [ 360553, 360610 ], "stop": [ 360553, 360610 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024112F2E", "query_cov": 1.0, "subject_cov": 0.7037037037037037, "identity": 0.9740000000000001, "score": 151.0, "evalue": 4.36e-46, "gene_start": 1, "gene_end": 76, "reference_start": 33, "reference_end": 108, "reference_sequence": "MTPYKKAIDLWKNYYDSYSIGLKEVEEIIWDKLYINGYTIFFKPKKIYALINQPELDKELTETTDDYKFKLMLVPKENTIEHNKLIENFKNYCDDLMNRSKKLKNDNQ" }, "paralog": false, "identity": 0.9629629629629629, "score": 213.0, "evalue": 4.74e-73, "description": "Internal stop codon at 360553, 360610. Nonsense mutation around 360553, 360610.", "psc": { "uniref90_id": "UniRef90_UPI0024112F2E", "uniref50_id": "UniRef50_UPI0024112F2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024112F2E", "UniRef:UniRef50_UPI0024112F2E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024112F2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024112F2E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9209.470799999992, "isoelectric_point": 7.784702491760254 }, "id": "AHCKFKLKGB_642", "locus": "GCA013367975_00628" }, { "type": "cds", "sequence": "contig_1", "start": 360864, "stop": 361283, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGAACAAAAATTGTAACCTCATTAAGTCTTTGCTTAACTACCGCAATTTCAACAATAGCAATCTATTCAATAATCGCCGTTAAAAAAAATATAGAACTTGATAAGTCAAATAAATATTCATTATTTATACAAGAAAAATCTAAATTGCAATCGCTTATCTCAAATAATCAACACAAAATTCCAAATAGCTATTTAAATTCAATTCAATATTTTCATAATATAACTAAAACCTCAAAAGCAATAGAAATAATTGATGCAACATCAAAAATAAGAAATGAAATTATTGACGTAAATGAAAAAATTCTTGCCTTTGATGTAGAAATAAAAAATAAACTATCGCTATTCAATGAATTTAATCAAATAAAACAAAAGCTTGAAAATGATTACCATAACAGACTATATAAAATATGA", "aa": "MKKRTKIVTSLSLCLTTAISTIAIYSIIAVKKNIELDKSNKYSLFIQEKSKLQSLISNNQHKIPNSYLNSIQYFHNITKTSKAIEIIDATSKIRNEIIDVNEKILAFDVEIKNKLSLFNEFNQIKQKLENDYHNRLYKI", "aa_hexdigest": "75db02841b22d49afb8d2f619d4f2c18", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16165.607699999988, "isoelectric_point": 9.60606327056885 }, "id": "AHCKFKLKGB_643", "locus": "GCA013367975_00629" }, { "type": "cds", "sequence": "contig_1", "start": 361434, "stop": 362183, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J897", "UniRef:UniRef90_D1J897" ], "nt": "TTGAAAAATATTGAAAATAATATTGATTATTTTTTAGTTAAAAAAGAAGTTGAACATTTATTTGAAAAAATTGAAAGCACTATTTCAATAGTTGAAACTAGACTAGAAAAAAATAAAATCAATAAAATATTTGAGGATAATGAAGTAAAAGAAATTTTAGATTTAATTTCACAGTTTAAATTAGATTTACAAAAACAAAAAGATTTATATAACTCAGTAGACAATAAAACCGATAACATCAAACACGAAACTTTTAAAAAAATATTTAGCCATTATAATAAAAACAATTTCTTTAAAATTTTTGATTGCATAGATCAAAGAGTTGATTTATATTACGACTTTATGTTCTTTTACTCACCTTTATATTTTTATGAACCAACAGAAGAAGTCAAAAAAGTTTTCCAATATCCTGATAAAAAAGATTTTGAAAAAGAAATAATTTTAAAATATAATCAAGCCAAACAAGATCTTACTAACTTCAAATCAAAGATAAATGGAAATGAAAAAATTGCCAAAAAAGCATTCAATAAATTCAATGAATATATACGCAATTTAGCAATAAGTTTTGAAAAGGAATTAATACAAAAAAAATTATTATTATTAAAAAATTTATTAACCAAATATGATCAAAAGCAAGATGGAAAATCACAATTCATAACAGTTGAAATAAAAAAAGCCATAAATGATGCAGAATCTTTATTAAGTTATAATGACTTAAAATATTCCACAAGAGATAAAAAGAGTATTTGA", "aa": "MKNIENNIDYFLVKKEVEHLFEKIESTISIVETRLEKNKINKIFEDNEVKEILDLISQFKLDLQKQKDLYNSVDNKTDNIKHETFKKIFSHYNKNNFFKIFDCIDQRVDLYYDFMFFYSPLYFYEPTEEVKKVFQYPDKKDFEKEIILKYNQAKQDLTNFKSKINGNEKIAKKAFNKFNEYIRNLAISFEKELIQKKLLLLKNLLTKYDQKQDGKSQFITVEIKKAINDAESLLSYNDLKYSTRDKKSI", "aa_hexdigest": "627de341514400c1838716d157a2aaa9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 361134, "stop": 362234, "observations": { "mutation": [ 361281, 361383, 362181 ], "stop": [ 361281, 361383, 362181 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J897", "query_cov": 1.0, "subject_cov": 0.5460526315789473, "identity": 0.956, "score": 452.0, "evalue": 8.24e-157, "gene_start": 1, "gene_end": 249, "reference_start": 191, "reference_end": 439, "reference_sequence": "MKKRTKIVTSLSLCLTTAISTIAIYSIIAVKKNIELDKSNKYSLFIQEKSRLQSLVANNQHKIPNSYLNSIQYFHNVTRTSKAIEIIDATSKIRSEIIDVNKKILAFDLEIKNQLSLFNEFNQIKQKLENDYLNRLYKIWRADAGEPNMIVDFGFFEEETHFQKYLETIKKYSWISKNSSSFEIKRAIFDLKNIENNIDYFLVKKEVEHLLEKIESTISIVETRLEKNKINKIFEDNEVKEILDLISQFKLDLQKQKDLYNSVDSKTDNIKHETFKKIFSHYNKNNFFKIFDCIDQRVELYYDFMFFYSPLFFYEQTEEIKKVFQYPNKKDFEKEIILKYNQAKQNLTNFKSKINGNEKIAKKAFNKFNEYIRNLAISFEKELIQKKLLLLKNLLTKYDQKQDGKSQFITVEIKKAINDAESLLSYNDSQYSTRDKKSIWEFRVTKNNLQELIDKY" }, "paralog": false, "identity": 0.9344262295081968, "score": 678.0, "evalue": 2.23e-248, "description": "Internal stop codon at 361281, 361383, 362181. Nonsense mutation around 361281, 361383, 362181.", "psc": { "uniref90_id": "UniRef90_D1J897", "uniref50_id": "UniRef50_D1J897", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J897", "UniRef:UniRef50_D1J897" ] }, "pscc": { "uniref50_id": "UniRef50_D1J897", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J897" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 30062.13710000003, "isoelectric_point": 8.838115882873538 }, "id": "AHCKFKLKGB_644", "locus": "GCA013367975_00630" }, { "type": "cds", "sequence": "contig_1", "start": 362358, "stop": 362591, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA961", "UniRef:UniRef90_UPI00215DA961" ], "nt": "TTGAAATTGATATCATCAAATGTTGAGGAATTTAATATTCTTGCAAATGATGTCTATTCTAAAAACCAAAAAATTTCCAACGAAATACAAGAAGTTAGAAAACTTATAGAAGAACACGAAGAAAAATTAGAAGATTTTTATATTAAGAAATTGCAGCCAAATTTAAAATATTTAACAGAAATATTACTTAAATTACAAAATAAATTACATTTAATTAGACAAAAAGTCAATTAA", "aa": "MKLISSNVEEFNILANDVYSKNQKISNEIQEVRKLIEEHEEKLEDFYIKKLQPNLKYLTEILLKLQNKLHLIRQKVN", "aa_hexdigest": "e8a7d7028a877c04f314a8a61fbae7dd", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 362358, "stop": 362801, "observations": { "mutation": [ 362600 ], "stop": [ 362600 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215DA961", "query_cov": 1.0, "subject_cov": 0.5167785234899329, "identity": 0.935, "score": 132.0, "evalue": 3.25e-38, "gene_start": 1, "gene_end": 77, "reference_start": 71, "reference_end": 147, "reference_sequence": "MLNESLKEQKNSEDGNFLSKFLNNEIAKILKENSKLFSSSDIAKINKNIEITYELSRKLNLEIILNRWNLLKLISSNVEEFNILANDVYSKNQKISNEIQEVKKLIEEHDKKLEDFYIKKLQPNLKYLTEILLKLQNKLYLIRQKVNQI" }, "paralog": false, "identity": 0.9455782312925171, "score": 268.0, "evalue": 3.73e-94, "description": "Internal stop codon at 362600. Nonsense mutation around 362600.", "psc": { "uniref90_id": "UniRef90_UPI00215DA961", "uniref50_id": "UniRef50_UPI00215DA961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215DA961", "UniRef:UniRef50_UPI00215DA961" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA961" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9281.687599999992, "isoelectric_point": 7.953093910217285 }, "id": "AHCKFKLKGB_645", "locus": "GCA013367975_00631" }, { "type": "cds", "sequence": "contig_1", "start": 362598, "stop": 363188, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410C12B", "UniRef:UniRef90_UPI002410C12B" ], "nt": "ATGTTGAACGAATTATTTAATGATTTCAAGGATATTGATTCAAAAACATTTGCAGAAGTTATTTATCAATTTGAAGAATCAAATAAATTATTGGCCGAATATATTGAATTATATTCACAAATTATTTATAATGCAAAAAATGAATTTGAAATGCAACAAAAAAATAGCGATTTATTTAATTTATTTAGTCAAGAAACAAAAAATAAAATTACAGATTTTTTAGCAACAATTAATAAATTGGAAAATAATAGAAATGATCAACAAATAAAAAAATCTATACATGAAGAATTTAAAAAAATTCTAACTGTCCAAGAAGAAATTTATAAAAATATAAATAAGGATTTTTTAAACATTACTAATAAAGAAGAAAGCATTAAACTTTTAAAAATGCTTAATGAAAGTTTAAAAGAACAAAAAAATTCAGAAGATGGAAATTTTTTAAGTAAATTTCTAAATAATGAAATAGCAAAAATATTAAAAGAAAATTCTGAGCTATTTTCTTCAAAAGATATTGCCAAAATCAATAAAAATATGGAAATTACATACGAACTTTCTAGAAAATTAAACTTAGAAATTATTTTAAATAGGTGA", "aa": "MLNELFNDFKDIDSKTFAEVIYQFEESNKLLAEYIELYSQIIYNAKNEFEMQQKNSDLFNLFSQETKNKITDFLATINKLENNRNDQQIKKSIHEEFKKILTVQEEIYKNINKDFLNITNKEESIKLLKMLNESLKEQKNSEDGNFLSKFLNNEIAKILKENSELFSSKDIAKINKNMEITYELSRKLNLEIILNR", "aa_hexdigest": "85a5745bc453a8f1f3d6d95691d16624", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 362358, "stop": 363488, "observations": { "mutation": [ 362600, 363209, 363452 ], "stop": [ 362600, 363209, 363452 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410C12B", "query_cov": 1.0, "subject_cov": 0.47115384615384615, "identity": 0.9740000000000001, "score": 348.0, "evalue": 1.25e-117, "gene_start": 1, "gene_end": 196, "reference_start": 141, "reference_end": 336, "reference_sequence": "MKSKEILWNISVQKNLIDQRYTKESLSPKSKIDVEFLKQKRKLENLFSIYKKWNKADMSKEIELLKKINNVNFGFKNDDIAKLTSEISKRIEDIYQKINSSKDIQKHLNNVKNDLNPLIQELINFNNFKNSKTWDVVDRLMLNELFNDFKDIDSKTFAEVIYQFEESNKLLAEYIELYSQIIYNAKNEFEMQQKNSDLFNLFSQETKNKITDFLAIINKLENNRNDQQIKKSIHEEFKKILTVQEEIYKNINKDFLNITNKEESIRLLKMLNKNLKEQKNSEDGNFLSKFLNNEIAKILKENSELFSSKDIAKINKNIEITYELSRKLNLEIILNRWNLLKLISSNVEEFNILANDVYSKNQKISNEIQEVRKLIEEHEEKLEDFYIKKSQLILKYLTEILLKLQNKLHSIRQKVN" }, "paralog": false, "identity": 0.9601063829787234, "score": 691.0, "evalue": 2.96e-255, "description": "Internal stop codon at 362600, 363209, 363452. Nonsense mutation around 362600, 363209, 363452.", "psc": { "uniref90_id": "UniRef90_UPI002410C12B", "uniref50_id": "UniRef50_UPI002410C12B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410C12B", "UniRef:UniRef50_UPI002410C12B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410C12B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410C12B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23344.265700000033, "isoelectric_point": 5.226368141174315 }, "id": "AHCKFKLKGB_646", "locus": "GCA013367975_00632" }, { "type": "cds", "sequence": "contig_1", "start": 363207, "stop": 363437, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAGGAAATAGAACTTCTTAAAAAGATTAATAATGTTAATTTTGATTTCAAAAATGAAGATATAGCGAAATTAACTAGTGAAATTTCAAAAAGAATTGAAGACATTTATCAAAATATAAATTCATCAAAAGATATACAAAAACATTTAAATAATGTTAAAAATGATTTAAATCCGCTTATACAAGAATTGATTAACTTTAATAATTTTAAAAATAGTAAAACATGA", "aa": "MSKEIELLKKINNVNFDFKNEDIAKLTSEISKRIEDIYQNINSSKDIQKHLNNVKNDLNPLIQELINFNNFKNSKT", "aa_hexdigest": "e61829f9033391704181c4ef5797eabe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8951.050899999995, "isoelectric_point": 7.96650333404541 }, "id": "AHCKFKLKGB_647", "locus": "GCA013367975_00633" }, { "type": "cds", "sequence": "contig_1", "start": 363585, "stop": 364019, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CFBAA", "UniRef:UniRef90_UPI00215CFBAA" ], "nt": "ATGAAACAAAAAAACAAACTATTTATTCTATCAACTATCTTAGGATCGTTTGCAACTGTATTTACAGTAATTTCTAGTGCTTCATGTTCTAATAAACAAGATAAAAAAGAAGATTCAAACAACAATGTAGCAAATACACCTAATGTCAAGCCAAATATATCTAATGAAAAACAAGAAGATAATAGCCACATAATTAATCCTAATATAAAGCCAACTGTTCCAAATGAATCTCAAAAAACAAAAGAAGATAAAAATAATGCGGCAAGTCCTAGTCCTAGTAGGCTACAAGAAGTTAAAAATGAACTGCAAACATATATAGCCAATAATCTTAAAGATTTTATTTATGAAAATTTAAAATTAGAAATTAAAGATTTTATTGAAAAAGAAAAAAATGAGGCTGATGAATTTATTTTGAGATCTAAAGAAATTCTTTGA", "aa": "MKQKNKLFILSTILGSFATVFTVISSASCSNKQDKKEDSNNNVANTPNVKPNISNEKQEDNSHIINPNIKPTVPNESQKTKEDKNNAASPSPSRLQEVKNELQTYIANNLKDFIYENLKLEIKDFIEKEKNEADEFILRSKEIL", "aa_hexdigest": "6fe3c6263f4429b38c93e5790bc07874", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 363456, "stop": 364019, "observations": { "mutation": [ 363587 ], "stop": [ 363587 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215CFBAA", "query_cov": 1.0, "subject_cov": 0.7700534759358288, "identity": 0.868, "score": 227.0, "evalue": 8.71e-74, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MKRKNKLFILSTILGSFATVFTVISSASCSNKQDRKEDSNNNVANTPNVKPNISNEKQEDNSHIINPNKKPTAPNESKKPKEDKNNVASSSPSRLQEVKNELQTYIANNLKDFIYENLKLEIKDFIEKEKNEADEFILKSREILWNISVQKNLIDQRYTKESLSPKSKIDVEFLKQKRKLENLFSIY" }, "paralog": false, "identity": 0.8877005347593583, "score": 320.0, "evalue": 3.37e-113, "description": "Internal stop codon at 363587. Nonsense mutation around 363587.", "psc": { "uniref90_id": "UniRef90_UPI00215CFBAA", "uniref50_id": "UniRef50_UPI00215CFBAA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215CFBAA", "UniRef:UniRef50_UPI00215CFBAA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CFBAA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CFBAA" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16369.186099999992, "isoelectric_point": 6.613694190979005 }, "id": "AHCKFKLKGB_648", "locus": "GCA013367975_00634" }, { "type": "tRNA", "sequence": "contig_1", "start": 364229, "stop": 364312, "strand": "+", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 70.9, "nt": "GGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 364235, 364237 ], "id": "AHCKFKLKGB_20", "locus": "GCA013367975_00635" }, { "type": "tRNA", "sequence": "contig_1", "start": 364316, "stop": 364390, "strand": "+", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 65.1, "nt": "TGCCCTTTAGCCAAGTGGAAAGGCAGCGGTTTTTGGTACCGCCATCCGTTAGTTCGAATCTAACAAGGGCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 364348, 364350 ], "id": "AHCKFKLKGB_21", "locus": "GCA013367975_00636" }, { "type": "cds", "sequence": "contig_1", "start": 364495, "stop": 364716, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00093A2145", "UniRef:UniRef90_UPI00093A2145" ], "nt": "TTGAGTAACACCAAAAATACTACATCTTCTTTTATTGAAAATAAGCTTAAAGGAAAAGCTGAATATAAAGAAGGCTACGATGCTTTAAACAAATTAATACAAGAAGCTAAAGAAATTTTGAAGAATTCAGGAAAACATAGCAATTTAGAACTAAAAAATAAAGAAGCTGAAATACAAGCCAAATTTATTGAATATAAAAATAAATATAACATTAACAAATAA", "aa": "MSNTKNTTSSFIENKLKGKAEYKEGYDALNKLIQEAKEILKNSGKHSNLELKNKEAEIQAKFIEYKNKYNINK", "aa_hexdigest": "ed31934871a94568d9e88f77c46e7a67", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 364495, "stop": 364935, "observations": { "mutation": [ 364725, 364839, 364893 ], "stop": [ 364725, 364839, 364893 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00093A2145", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.877, "score": 119.0, "evalue": 3.71e-33, "gene_start": 1, "gene_end": 73, "reference_start": 74, "reference_end": 146, "reference_sequence": "MRTDSSNCLDSKKYWEEAEKEISKIRWDALDLEKQGIQEDKLEKFKSDFRALKKPIEDKKNEILDEFADFWTKLSNSKNKTSYFIENKLKGKAEYKEGYDALNKLIQEAKEILNNSEKHSNLELKNKEAEIQAKLLEYKNKYNMNK" }, "paralog": false, "identity": 0.863013698630137, "score": 242.0, "evalue": 6.23e-84, "description": "Internal stop codon at 364725, 364839, 364893. Nonsense mutation around 364725, 364839, 364893.", "psc": { "uniref90_id": "UniRef90_UPI00093A2145", "uniref50_id": "UniRef50_UPI00093A2145", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00093A2145", "UniRef:UniRef50_UPI00093A2145" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00093A2145", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00093A2145" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8453.506099999995, "isoelectric_point": 9.426518821716307 }, "id": "AHCKFKLKGB_649", "locus": "GCA013367975_00637" }, { "type": "cds", "sequence": "contig_1", "start": 365128, "stop": 369012, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CD68537", "UniRef:UniRef90_UPI000CD68537" ], "nt": "ATGAAAAAATCGAAATTAAGAATTTTACTTTCTACTTCGTTAATTATTGCTTTAGCAACAACAGCAACTTTAGTTGCAACATCACTAAAAAGTAAATTTAAAAGAAGACAATATGAACAAGAAGATAATATTGACAAATTAAAGGAAAAATTTAATAGATCAAAATCTAAATTAGATAATTTAATTAAATCAAATGAAGCCAATGATGTTGATAAACAACCAGAAACCAACATCTTTAACAATACAAATCTTACTGATAAAGATTCAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTAACTAAGAAAATTAATGATAAAAAGAACCAAAAAGACAACCTTCTTAAAGATTTTAACGATGCTAAAAAGAAATTGCAAGATTTAATTAATTCACAAGACGGCCAAAAAGTTGACACTTCTAAAGCAAATCAATCATTGCAAAATAACAATGTAGATGCTTCATCAACAGTCGATCAAATTGTTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAACAAGCTAAACAAGACTTTAATTCAAAGAAACAACAACTAGATAAATTAATTAAATCAAATGATGCCAATGATGTTGATAAACAAGAAGAAACCAACATCTTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTAACTAAGAAAATTAATGATAAAAAAGACAACCTTCTTAAAGATTTTAACGACGCTAAAAAGCAATTAAAGGATTTAATTGATTCACAAGACGGTCAAAAAGTTGACACATCTAAAGCAAATCAATCATTGCAAAACAACAATGTAGATGCTTCATCAACGAGCGATCAAATTGTTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAACAAGCTAAACAAGACTTTAATTCAAAGAAGCAACAACTAGATAAATTAATTAAATCAAATGATGCCAATGATGTTGATAAACAACCAGAAACCAACATCTTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTGACTAAGAAAATTAATGATAAAAAAGACAATCTTCTTAAAGATTTTAATGATGCTAAAAAGCAATTAGAAGACTTAATTAATTCACAAGACGCACAAAAAGTCGACACTTCCAAAGCAAATCAATCATTGCAAAACAACAATGTAGATGCTTCATCAACAGTCGATCAAATTGTTCATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAAAAAGCCACACAAGAATTTAATTCAAAGAAACAACAATTAGATAATTTAATTAATTCAAATGAAGCAAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTAACTAAGAAAATTAATGATAAAAAAGACAATCTTCTTAAAGATTTTAATGATGCTAAAAAGCAATTAGAAGACTTAATTAATTCACAAGACGCACAAAAAGTCGACACATCTAAAGCAAATCAATCATTGCAAAACAATAATGCAGATACTTCATCAACGACCGATCAAATTGTTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGAAGCCAAAGAAAATGCTAAACAAGAATTTAGTTCAAAGAAGCAACAATTAGATAATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAACCAGAAACTGACATATTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTAACTAAGAAAATTAATGATAAAAAAGACAATCTTCTTAAAGATTTTAATGATGCTAAAAAGCAATTAGAAGACTTAATTAATTCACAAGACGCACAAAAAGTCGACACATCTAAAGCAAATCAATCATTGCAAAACAATAATGCAGATACTTCATCAACGACCGATCAAATTGTTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGAAGCCAAAGAAAATGCCAAACAAGACTTTAATTCAAAGAAGCAACAATTAGATGATTTAATTAATTCAAATGAAGCCAAAGATGTTGATAAACAAACAGAAACTGATATCTTTAACAACACAAATCTTTCTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTGACTAAGAAAATTAATGATAAAAAAGACAATCTTCTTAAAGACTTTAACGACGCTAAAAAGAAATTACAAGATTTAATTAATTCACAAGACGGTCAAAAAGTTGACACATCTAAAGCAAATCAATCATTGCAAAACAATAATGCAGATACTTCATCAACGACCGATCAAATTGTTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGAAGCCAAAGAAAATGCTAAACAAGAATTTAGTTCAAAGAAACAACAATTAGATAATTTAATTAATTCAAATGAAGCCAAAGATGTTGATAAACAAGAAGAAACCAACATCTTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTAACTAAGAAAATTAATGATAAAAAAGACAATCTTCTTAAAGATTTTAATGATGCTAAAAAGCAATTAGAAGACTTAATTAATTCACAAGACGGTCAAAAAGTTGACACATCTAAAGCAAATCAATCATTGCAAAACAATAATGCAGATACTTCATCAACGACCGATCAAATTGTTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCTAAAGAACAAGCTAAACAAGACTTTAATTCAAAGAAACAACAACTAGACAATTTGATTAAATCAGATGAAGCCAAAGATGTTGATAAACAAGAAGAAACCAACATCTTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAGTCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTGACCAAAAAAATTAATGATAAAAAAGAAAATCTTCTTAAAGACTTTAATGACGCTAAAAAGCAATTAGAAGACTTAATTAATTCACAAGACGGTCAAAAAGTCGACACATCTAAAGCAAATCAATCATTGCAAAACAATAATGCAGATACTTCATCAACGACCGATCAAATTGTTCATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCTAAAGAACAAGCTAAACAAGACTTTAATTCAAAGAAACAACAACTAGACAATTTGATTAAATCAGATGAAGCCAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAACACAAATCTTTCTGGTAATGATTTAATTAAAGACTTTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTGACTAAGAAAATTAATGATAAAAAGCCAAAGGAAAATATTGAATATGAAGGACTTGAAAAAATCAGGGAACAAATTAAACAATTTATCGAAAAAGTAAAAGAGGATGAATATTATACAAAATATAAAAATCAACAATATTATGATTGA", "aa": "MKKSKLRILLSTSLIIALATTATLVATSLKSKFKRRQYEQEDNIDKLKEKFNRSKSKLDNLIKSNEANDVDKQPETNIFNNTNLTDKDSIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKKLQDLINSQDGQKVDTSKANQSLQNNNVDASSTVDQIVNATNEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDKLIKSNDANDVDKQEETNIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLKDLIDSQDGQKVDTSKANQSLQNNNVDASSTSDQIVNATNEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDKLIKSNDANDVDKQPETNIFNNTNLTGNDLIKDIESKTKTIEDAIKSLTKKINDKKDNLLKDFNDAKKQLEDLINSQDAQKVDTSKANQSLQNNNVDASSTVDQIVHATTEIKKATQDLQKLIDAAKEKATQEFNSKKQQLDNLINSNEAKDVDKQPETDIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLEDLINSQDAQKVDTSKANQSLQNNNADTSSTTDQIVNATNEIKKATQDLQKLIDEAKENAKQEFSSKKQQLDNLIKSNEAKDVDKQPETDIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLEDLINSQDAQKVDTSKANQSLQNNNADTSSTTDQIVNATNEIKKATQDLQKLIDEAKENAKQDFNSKKQQLDDLINSNEAKDVDKQTETDIFNNTNLSGNDLIKDIESKTKTIEDAIKSLTKKINDKKDNLLKDFNDAKKKLQDLINSQDGQKVDTSKANQSLQNNNADTSSTTDQIVNATNEIKKATQDLQKLIDEAKENAKQEFSSKKQQLDNLINSNEAKDVDKQEETNIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNADTSSTTDQIVNATNEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDNLIKSDEAKDVDKQEETNIFNNTNLTGNDLIKDIESKTKTIEDAIKSLTKKINDKKENLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNADTSSTTDQIVHATTEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDNLIKSDEAKDVDKQPETDIFNNTNLSGNDLIKDFESKTKTIEDAIKSLTKKINDKKPKENIEYEGLEKIREQIKQFIEKVKEDEYYTKYKNQQYYD", "aa_hexdigest": "a605b34c1721121d1f0a4070daabcc40", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00193946BF", "query_cov": 1.1143740340030912, "subject_cov": 0.875, "identity": 0.742, "score": 1414.0, "evalue": 0.0, "valid": false, "uniref50_id": "UniRef50_UPI000CD68537" }, "pscc": { "uniref50_id": "UniRef50_UPI000CD68537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CD68537" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 364825, "stop": 369003, "observations": { "insertion": [ 365325 ], "deletion": [ 365240 ], "mutation": [ 364838, 364892, 365129 ], "stop": [ 364838, 364892, 365129, 365276 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000CD68537", "query_cov": 0.9505409582689336, "subject_cov": 0.6886898096304591, "identity": 0.872, "score": 1458.0, "evalue": 0.0, "gene_start": 1, "gene_end": 1230, "reference_start": 1, "reference_end": 1227, "reference_sequence": "MKKSKLRILLSTSLIIALATTATLVATSLKSKFKRRQYEQEDNIDKLKEKFNRSKSKLDNLIKSNEAKDVDKQPETDILNNTNHTGNDLTKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDTSSTTDQIINATNEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDNLIKSDEAKDVDKQPETNIFNNTNLTDKDLIKDIESKTKTIEDAIESLTKKINDKKDSLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDASSTVDQIVHATTEIKKATQDLQKLIDAAKEKATQDFNSKKQQLDDLIKSNEAKDVDKQTETDIFNNTNLSGNDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKKLQDLINSQDGQKVDTSKANQSLQNNNVDASSTTDQIINATTEIKKATQDLQKLIDAAKENAKQEFTSKKQQLDNLIKSNEAKDVDKQPETNIFNNTNLTDKDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLEDLINSQDAQKVDTSKANQSLQNNNVDASSTVDQIVHATTEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDNLIKSDEAKDVDKQPETNIFNNTNLTDKDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDASSTVDQIVHATTEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDNLIKSDEAKDVDKQPETNIFNNTNLTDKDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDASSTVDQIVHATTEIKKATQDLQKLIDAAKEKATQEFTSKKQQLDNLIKSNEAKDVDKQPETNIFNNTNLTDKDLIKDIESKTKTIEDAIESLTKKINDKKDSLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDASSTVDQIVHATTEIKKATQDLQKLIDAAKEKATQDFNSKKQQLDDLIKSNEAKDVDKQPETNIFNNTNLSGNDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKKLQDLINSQDGQKVDTSKANQSLQNNNVDASSTTDQIINATTEIKKATQDLQKLIDAAKEKATQEFTSKKQQLDNLIKSNEAKDVDKQPETNIFNNTNLTDKDLIKDIESKTKTIEDAIESLTKKINDKKDSLLKDFNDAKKQLEDLINSQDAQKVDTSKANQSLQNNNVDASSTVDQIVHATTEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDNLIKSDEAKDVDKQPETNIFNNTNLTDKDLIKDIESKTKTIEDAIESLTKKINDKKDSLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDASSTVDQIVHATTEIKKATQDLQKLIDAAKEKATQDFNSKKQQLDDLIKSNEAKDVDKQTETDIFNNTNLSGNDLIKDIESKTKTIEDAIKSLTKKINDKKPKENIEYEGLEKIREQIKQFIEKVKEDEYYTKYKNQQYYDWDSNIIDHLNRQLEFFKNVTSASDIQQISGAKQQLTKDLNKAKKDKFSSDIYCFVNKEVTKFTGDMRTDSSNCLDSKKYWEEAEKEISKIREDAIDLWKQEPQEDKLEKFKSDFRALKKPIEDKMNEIIVEFFDFWKKLSNSKNKTSYFIENKLKGKAEYKEGYDALNKLIQEAKEILNNSEKHSISELKNKEAEIQAKLLEYKNKYNMNK" }, "paralog": false, "identity": 0.8488538681948424, "score": 1803.0, "evalue": 0.0, "description": "Internal stop codon at 364838, 364892, 365129, 365276. Frameshift due to insertion around 365325. Frameshift due to deletion around 365240. Nonsense mutation around 364838, 364892, 365129.", "psc": { "uniref90_id": "UniRef90_UPI000CD68537", "uniref50_id": "UniRef50_UPI000CD68537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000CD68537", "UniRef:UniRef50_UPI000CD68537" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000CD68537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CD68537" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 146064.47790000084, "isoelectric_point": 5.118658256530761 }, "id": "AHCKFKLKGB_650", "locus": "GCA013367975_00638" }, { "type": "cds", "sequence": "contig_1", "start": 369268, "stop": 370317, "strand": "-", "frame": 1, "gene": null, "product": "MSC_0618 family F1-like ATPase beta subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0004864FE0" ], "nt": "ATGAATTCGACTATAAACAATGAAAGATATCTAGAAAATAACAAAGAAGTTATAGAAACCGGAATAAAGGCAATTGACTTCTTTATGCCAATCGTTAAGGGCGCAAAAATTGGTTTATTCGGAGGAGCCGGAGTTGGAAAAACTGTTCTAATGAAGGAAATTATTTTTAACCTTAATAAACAACATGAAGCAAATTCTAATATCTTTATTGGTTCAGGTGAACGTTCAAGAGAAGGAATAGAATTATACAATGAACTTTCTTCATCTAATTTAATGAAGGACTCAATAATGTTTATTTCAAAAATGAATGAAGCGCCAGGAGCTAGAACGTCAATTGTTCCAATCGGAATTACTGCTGCAGAATATTTAAGAGACACATGCAATGAAAATGTTTTATTATTTATTGACAATATTTATCGTTTTATTCAAGCAGAAAATGAAGTAAGTTCAGCATTAGGCAAAAAGCCTTCAATTGGAGGTTATCAATCAACTTTAGAAAGTGATGTAACTGAAGTTGAAAGCAGATTGTTTAGAAACAAGAAGGGCTCAATAACTTCATTCCAAACAATATTTCTACCAATGGATGATTTAAGCGACCCTTCTGCAGTAGCTGTATTTAATCACTTAGATGGTAATTTAGTTTTATCTAGATCTCAAGCAGCTAAAAATATTTTTCCTGCCTTTGATCCACTTGCTTCATCTACAAACGCAATTAATCCGACCATTATTGGCAAACGCCACTATGATGCAATAATTGAAGCAAAAAGAATATTAAAAGCATATAAAGATCTTGAAGATGTTATTCTAATATTGGGATTTGATGAACTTGATGAAGAAAGCAAAATCACTGTTAAAAAAGCTCTTCAACTCGAAATGTTTTTTACCCAAAATTTCTTTATGACTGAACACTTTACTAAATCGCCCGGTCAATTTGTACCATTAAACGAAACAGTTGAAAGCGTTATAAGAATATTAGAAGGAAGATACTTAAAACAAAATCCTGAAATTTTTGCCTATATAGGTTCAAATAAAGATATTCAACAAGATTAA", "aa": "MNSTINNERYLENNKEVIETGIKAIDFFMPIVKGAKIGLFGGAGVGKTVLMKEIIFNLNKQHEANSNIFIGSGERSREGIELYNELSSSNLMKDSIMFISKMNEAPGARTSIVPIGITAAEYLRDTCNENVLLFIDNIYRFIQAENEVSSALGKKPSIGGYQSTLESDVTEVESRLFRNKKGSITSFQTIFLPMDDLSDPSAVAVFNHLDGNLVLSRSQAAKNIFPAFDPLASSTNAINPTIIGKRHYDAIIEAKRILKAYKDLEDVILILGFDELDEESKITVKKALQLEMFFTQNFFMTEHFTKSPGQFVPLNETVESVIRILEGRYLKQNPEIFAYIGSNKDIQQD", "aa_hexdigest": "aa26a2106ae8e3530029006e22d0177b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0004864FE0", "query_cov": 0.8997134670487106, "subject_cov": 0.9843260188087775, "identity": 0.7609999999999999, "score": 485.0, "evalue": 2.05e-170, "valid": false, "product": "MSC_0618 family F1-like ATPase beta subunit", "uniref50_id": "UniRef50_UPI0004864FE0" }, "pscc": { "uniref50_id": "UniRef50_UPI0004864FE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0004864FE0" ], "product": "MSC_0618 family F1-like ATPase beta subunit" }, "genes": [], "id": "AHCKFKLKGB_651", "locus": "GCA013367975_00639" }, { "type": "cds", "sequence": "contig_1", "start": 370363, "stop": 370545, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACATTGCACGAAGGGAAAACTTTTTTATTAGTAAAAAGATTAATTGACGAAAAACATGTAAGAGCAATTGTTGTATATGCAAGTAAAGATATTTCAATTAACGAAGAAGTTGTTAACACTCATAAGAGTTTTTTGGTTCCAATAGGCCAAAATTCAAAAAACAATATTTACAACTTCTGA", "aa": "MTLHEGKTFLLVKRLIDEKHVRAIVVYASKDISINEEVVNTHKSFLVPIGQNSKNNIYNF", "aa_hexdigest": "513b0d67c45413b864e932aa99aae246", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6912.9229, "isoelectric_point": 9.157943534851075 }, "id": "AHCKFKLKGB_652", "locus": "GCA013367975_00640" }, { "type": "cds", "sequence": "contig_1", "start": 370626, "stop": 370889, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGGAATAAAAGATGAACAAAAAGCATTTATGTTACTAGATTATTTAATAAATAATTATAATGATTGCATAAAAGCATTTAAAACAATTTTAGATAATTTAGACTACGATGATAATATTATGAAGAATTATTTTGCATTTGTCTTGAAATCATATTCAGATTATTTGAATTTAAATTGGGATATTGAATTAGAACATAATTTTGTGCCAATACCTGAAGAACAATTAGATAAGGCAGCGAAGGTTTTAGGTGATAAATAA", "aa": "MQGIKDEQKAFMLLDYLINNYNDCIKAFKTILDNLDYDDNIMKNYFAFVLKSYSDYLNLNWDIELEHNFVPIPEEQLDKAAKVLGDK", "aa_hexdigest": "778e24cd557c36216d7974e19d905744", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10302.607499999995, "isoelectric_point": 4.415900993347169 }, "id": "AHCKFKLKGB_653", "locus": "GCA013367975_00641" }, { "type": "cds", "sequence": "contig_1", "start": 370896, "stop": 371840, "strand": "-", "frame": 2, "gene": null, "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FAQ0" ], "nt": "ATGTTGAGCAATCATCATCCAATATTTGGCCTAGCTCATAATTTGATGACAGTTAAAACATTAAATGAACAACTTTATACCGGAATTATTGCAATAGATTTATTAATTCCAATCGGCAAAGGTCAACGTGAACTAATTATTGGCGATAGGCAAACAGGAAAAACTCATATGGCATTAAATGCAATTATAAATCAAGCAAAAAATGGCACAAAATGTATTTATGTTGCAATTGGCCAAAAGCGTGAATCATTAACTAATATTTATGAAACATTAAAAAAATACGATGCATTGAAAAACACTATGATTATTGATGCGCCTCAAAACGTTTATGAACAATATCTAGCGCCTTACATCGGAATGGCCCATGCAGAAAATCTTTCATATGACAATGATGTTTTAATAGTATTTGATGATTTAACTAAACATGCTAATATTATTAGAGAAATTGCCCTATTAACCAACAAGTCAGTTGGAAAAGAAGCAATGCCTAGTGACATCTTTTTTGCCCACTCATCATTGCTTGAAAGAGCTGGATCTTTTGTTAATAGAAAAACAATTACTGCACTTCCAATTTTGCAAACAGTTGACGGTGATATAACTAGTTTAATTTCATCTAATATAATTTCAATAACTGATGGTCAAATTGTAACTTCTTCTGACTTATTTGCTTCAGGGAAATTACCTGCTATAAACATTGATTTATCAGTATCAAGAACAGGTTCAAGTGTTCAATCAAGAATGATGACAAAAATAGCTGGTGAAATTAATAAAATTTTTAGAAGTTACAAGAGACATTTAAAATTGGCAATGTTAGATTACGAGCTTAATAAAGAAACTTCTATTTTGCTTCACAAAGGAAAATTAATTGACAAAATGTTCTTGCAAAAGGGTTTTTCACTTTACTCATATCGTTTTGTAGTGCTAACAACAAAATTAATTTCTTGA", "aa": "MLSNHHPIFGLAHNLMTVKTLNEQLYTGIIAIDLLIPIGKGQRELIIGDRQTGKTHMALNAIINQAKNGTKCIYVAIGQKRESLTNIYETLKKYDALKNTMIIDAPQNVYEQYLAPYIGMAHAENLSYDNDVLIVFDDLTKHANIIREIALLTNKSVGKEAMPSDIFFAHSSLLERAGSFVNRKTITALPILQTVDGDITSLISSNIISITDGQIVTSSDLFASGKLPAINIDLSVSRTGSSVQSRMMTKIAGEINKIFRSYKRHLKLAMLDYELNKETSILLHKGKLIDKMFLQKGFSLYSYRFVVLTTKLIS", "aa_hexdigest": "191f1297b78296469ae700fd94d6d26a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FAQ0", "query_cov": 0.8853503184713376, "subject_cov": 0.8938906752411575, "identity": 0.525, "score": 251.0, "evalue": 1.05e-78, "valid": false, "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein", "uniref50_id": "UniRef50_A0A432FAQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A432FAQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FAQ0" ], "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_654", "locus": "GCA013367975_00642" }, { "type": "cds", "sequence": "contig_1", "start": 371874, "stop": 372164, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAACACAACTAAAAATCCTCGTATTAAATCAATATTCAATTGCATAGCAACAATTGAGGGTGAATACAACTACCGTCAAAATCAACAATTTTATTTAGCAAGTAACCCAAATGTTAAATTCATAGTTATTTCTGCTACAACTAATAAGGCATTTATGTTGATTAATAGTGAACACGTATCTTTAGATATAAATAGTGAATTAATTGAATTAAATGAATCCCAAAATATAAATACTAGTCTTGATTATTTGGGCAAAATTATTGATATTAATGGTGGCGTCATTTGA", "aa": "MNNTTKNPRIKSIFNCIATIEGEYNYRQNQQFYLASNPNVKFIVISATTNKAFMLINSEHVSLDINSELIELNESQNINTSLDYLGKIIDINGGVI", "aa_hexdigest": "689894a023341db3c52f8aa6e8fc1b8f", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10842.134999999991, "isoelectric_point": 5.187547111511229 }, "id": "AHCKFKLKGB_655", "locus": "GCA013367975_00643" }, { "type": "cds", "sequence": "contig_1", "start": 372180, "stop": 372509, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTGGTATAAAACATAATTTTGACTTATTATCAAATTTAGCAAGTAATATTTCAATAAAAGCTAATAAAGAAGAAAAAGAAAATTTCAATAAAGCTTATTTAGAAGCAGTCAATGTTTTAAATAAAAATTATCTTGAACAAAAGAAGATTATTAAAAAACTAGGGACTTCTTTTATAGTTATTTCTTTAATCATAATAATTACAAACATAATAATTTTTGCATTGTTAAAAAACTTAAAATTAAAGAAAAAGGTAATTATTATCAATAGTGTAATACTAGCGATCGCATTACTAATATTAATAATTGGAGGGCTATTATTTATATAG", "aa": "MSGIKHNFDLLSNLASNISIKANKEEKENFNKAYLEAVNVLNKNYLEQKKIIKKLGTSFIVISLIIIITNIIIFALLKNLKLKKKVIIINSVILAIALLILIIGGLLFI", "aa_hexdigest": "40d5d3327595f25768d062261b24a46e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12201.784299999981, "isoelectric_point": 9.948970603942868 }, "id": "AHCKFKLKGB_656", "locus": "GCA013367975_00644" }, { "type": "cds", "sequence": "contig_1", "start": 372531, "stop": 372704, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATTGCAATTTAGTGAAGCGCTTAAATTCAATGAAAAATTCATTAAAACAAATTTAGCAGAAATCAAAGGCAATGAGAATGTTTTAAGAGAATTATTCTTATTAAATAAACAATTAAACTACAAATTTACCGCTTTAATTAGCCAAAGAGATTTCAACCAAGAAAAATGA", "aa": "MQLQFSEALKFNEKFIKTNLAEIKGNENVLRELFLLNKQLNYKFTALISQRDFNQEK", "aa_hexdigest": "d6eabfa7a88a7a82b8c32cda74619552", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6837.8099999999995, "isoelectric_point": 9.156976509094239 }, "id": "AHCKFKLKGB_657", "locus": "GCA013367975_00645" }, { "type": "cds", "sequence": "contig_1", "start": 372852, "stop": 373427, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCGATTTAAAACCATTGGTTGATGGTGATAAAACAAGCCACTTAACAAAAGAACAAGAAAAAAATTTAATTCAATCATTGCCCCTTTTGGTTCCGTTTATATCAAGCAACAATTTGCCTTCTTCATTAAGTCAAATTAAAAGCCAATTTGATTCATTAGATGCAAGCAAACGTCAAGAATTATTTTATTTCAATAACCCGATAAAAACTCGCTATAAATATTCTGTTTTTTCATTTGATATTAGTGGCAATGATATTATTAATTTAAAAGTACATATTTCAGATAATATAAATCCTAAGTTAGAAAGAACATATTTAATTGAAAAATATACTCCGTCACAAGACAAATATTTTAATCTGTTAAAAGAAACTGAAATAAAATCAATTTCAGAAATATTTAAGCCATTATACAAAGGTTTAGGCTTAGATGAAAAGTTAGATTACAATAAGCTAAGAAATGGATATTTAAGAAAAGCATTGCTTGCAATGATTGACTCAGCGCTACAAATGTCATTTAAAGCTGATAAAGATTCATATAGTGCAATTAGCAATAAAATATTGCAAAAATATTGA", "aa": "MPDLKPLVDGDKTSHLTKEQEKNLIQSLPLLVPFISSNNLPSSLSQIKSQFDSLDASKRQELFYFNNPIKTRYKYSVFSFDISGNDIINLKVHISDNINPKLERTYLIEKYTPSQDKYFNLLKETEIKSISEIFKPLYKGLGLDEKLDYNKLRNGYLRKALLAMIDSALQMSFKADKDSYSAISNKILQKY", "aa_hexdigest": "b52ed6c28258a28bdad0896d4ad5648d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22073.087600000003, "isoelectric_point": 9.125258064270021 }, "id": "AHCKFKLKGB_658", "locus": "GCA013367975_00646" }, { "type": "cds", "sequence": "contig_1", "start": 373536, "stop": 374234, "strand": "-", "frame": 2, "gene": null, "product": "MSC_0620 family F1-like ATPase-associated subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D97E37", "UniRef:UniRef90_UPI0034D97E37" ], "nt": "ATGAATAAAAAGAAAATCTTATTATTGTCATTTGCAACAGTTCCTTTATTATCTCCAATAGTAGTTTCAGCTGCGTCAAATAATGATCCAAATAATAAAAAACCTGAGTTAGATCCAAATTTTGGTGAATTTGAAAGTGAAGCTAAAAAAACAATTTCTTCAACAATAGAAGATGGTTTAAAAGCAATAATCAATTATTTGCAAGTTCAACAACAAAGATTGCTAGAAAATAAAGAATTAGAGTTTAAATTAAAAATTCAACAATTAATTTATTTAAAAAATTTAGAAACATTTCTAACAAAAAATAAAGAAGAGATTCAAAAAAATCCTAATAAGTATGGAATTTATTTAAATACTCCTCTAATATTAGGTAAAGAAAAAAACCATGATTTGCAGGATATTGATTACGAAGGCGATTTATTTAAATCAATAAAAACTGGTAAATCCGATCCATTAGATTATAAAAAAGCAATTTTGCCAAAAGGAAGTGTTAAAGAAGTTGCTAAAGATCAATTAAATACTGTTAAAAAAGATAAGTATTCTCAATTTTTAAAGAAATATAAATCTGATTTCTTAAAAGAAGTCAATAAGTTATTTTTTAATGAAAAAGATGTTTTAAATATTGACAAAGATGTCGAATTAATTAGGGATAAAAATGGAGTATTTTCTACCACCTTGCCAAAAGGCTTTAAAAGTTGA", "aa": "MNKKKILLLSFATVPLLSPIVVSAASNNDPNNKKPELDPNFGEFESEAKKTISSTIEDGLKAIINYLQVQQQRLLENKELEFKLKIQQLIYLKNLETFLTKNKEEIQKNPNKYGIYLNTPLILGKEKNHDLQDIDYEGDLFKSIKTGKSDPLDYKKAILPKGSVKEVAKDQLNTVKKDKYSQFLKKYKSDFLKEVNKLFFNEKDVLNIDKDVELIRDKNGVFSTTLPKGFKS", "aa_hexdigest": "01681d56c3b04e4f0aaa2ebe676b16fa", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 373233, "stop": 374234, "observations": { "insertion": [ 373634 ], "deletion": [ 373706 ], "mutation": [ 373537 ], "stop": [ 373537, 373646, 373667, 373673, 373700 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D97E37", "query_cov": 1.0, "subject_cov": 0.6609686609686609, "identity": 0.875, "score": 375.0, "evalue": 2.04e-128, "gene_start": 1, "gene_end": 232, "reference_start": 1, "reference_end": 232, "reference_sequence": "MNKKKILLLSFATVPLLSPIVVSAASNNDPNNKKPELDPNFGEFESEAKKTISSTIEDGLKAIINYLQVQQQRLLENKELEFKLKIQQLIYLKNLETFLTKNKEEIQKDPNKYGIYLNTPLILGKEKNHDLQDIDYEGDLFKSIKTGKTDPLDYKKAILPKGSVKEVAKDQLNAVKKDKYYQFLKKYKSDFLKEVNKLFFNEKDVLNIDKDVELIRDKNGVFSTTLPKGFKSWDEYFISKFKVRITKFDLKQNQETNEAEQKEEKNPDSLPDLKPLVDGDKTSHLTKEQEKNLIQSLPLLVPFISSNNLPSSLSQIKSQFDSLDASKRQELFYFNNPINTRYKYSVFFIWY" }, "paralog": false, "identity": 0.9279279279279279, "score": 586.0, "evalue": 4.01e-214, "description": "Internal stop codon at 373537, 373646, 373667, 373673, 373700. Frameshift due to insertion around 373634. Frameshift due to deletion around 373706. Nonsense mutation around 373537.", "psc": { "uniref90_id": "UniRef90_UPI0034D97E37", "product": "MSC_0620 family F1-like ATPase-associated subunit", "uniref50_id": "UniRef50_UPI0034D97E37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D97E37", "UniRef:UniRef50_UPI0034D97E37" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D97E37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D97E37" ], "product": "MSC_0620 family F1-like ATPase-associated subunit" } }, "id": "AHCKFKLKGB_659", "locus": "GCA013367975_00647" }, { "type": "cds", "sequence": "contig_1", "start": 374593, "stop": 374694, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTATATAAAATTGAATTACTTTTTTTAGAAAATAAGAAGCTAAATATTGAAAAAGGCTTAGTTTATATTAATGTCAATGAAGAAAATACATGA", "aa": "MNNLYKIELLFLENKKLNIEKGLVYINVNEENT", "aa_hexdigest": "bf89cffbd4c768243ac5d4ea37c19f42", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3954.544500000001, "isoelectric_point": 5.062103462219238 }, "id": "AHCKFKLKGB_660", "locus": "GCA013367975_00648" }, { "type": "cds", "sequence": "contig_1", "start": 374687, "stop": 375175, "strand": "-", "frame": 3, "gene": null, "product": "MSC_0622 family F1-like ATPase gamma subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR95", "UniRef:UniRef90_D1J8A4" ], "nt": "TTGCAATCTTATGAAAATTCAAATATTGAAAATTTGGCAGAAAAATTAACCAAAATAGTAATGATCTTGTATGAATATGAAAATTACGAAGAAGTCAATTTTGTAGTTAATTCTAATAAAAATTTTAATCAATATTTTACAATTTTGCCAATGTCTAAATTCTCGTTTGAAAAATTTCCTTTAAATGAAAGCAATAATAATTTATATGACATAGATTTTAAAAAAGCAAAAATATATCCTAATATTAATGATTTTATTAATTCTCAAATTAATTGTTATTTATTAAACACTATAAATATGTTAATATCTGAATCTGCATTTTATAAAGCAAAAATAGGTTTAGTTTCAACTAATACAATTTTAAAAGATTTAGACGAAAGTATTCTTAAACTTAAAAAGCAAATATCAAGAGCAAAAAGTGAATTACAAATTGAGGAACTAAATTTATTGACTAAAAAAGACCCTGATGAAGGTGATATTTATGAATAA", "aa": "MQSYENSNIENLAEKLTKIVMILYEYENYEEVNFVVNSNKNFNQYFTILPMSKFSFEKFPLNESNNNLYDIDFKKAKIYPNINDFINSQINCYLLNTINMLISESAFYKAKIGLVSTNTILKDLDESILKLKKQISRAKSELQIEELNLLTKKDPDEGDIYE", "aa_hexdigest": "78276d3a930eca3700b485055d598886", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 374687, "stop": 375475, "observations": { "mutation": [ 375328 ], "stop": [ 375328 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8A4", "query_cov": 1.0, "subject_cov": 0.5436241610738255, "identity": 0.9940000000000001, "score": 309.0, "evalue": 2.87e-104, "gene_start": 1, "gene_end": 162, "reference_start": 137, "reference_end": 298, "reference_sequence": "MQLKDLENKRKSLNNISLKVNNEKNIYLINIMKLNQRLSFFNKNALVSRQIILHLKEIYNIKNDMLRRESRHRFNLFRKINRNILWIYLTEEQKYSTDSYSRYEKTILSNIKKSNNEFILIGKNAIKFGQDNNLQVLQSYENSNIENLAEKLTKIVMILYEYENYEEVNFVVNSNKNFNQYFTILPMSKFSFEKFPLNESNNNLYDIDFKKAKIYPNINDFINSQINCYLLNTINMLISESAFYKAKIGLVSTNTILKDLDESILKLKKQISRAKSELQIEELNLLTKKDPDEGDIYE" }, "paralog": false, "identity": 0.9923664122137404, "score": 510.0, "evalue": 3.72e-186, "description": "Internal stop codon at 375328. Nonsense mutation around 375328.", "psc": { "uniref90_id": "UniRef90_D1J8A4", "product": "MSC_0622 family F1-like ATPase gamma subunit", "uniref50_id": "UniRef50_Q9PR95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8A4", "UniRef:UniRef50_Q9PR95" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR95" ], "product": "Uncharacterized protein UU050" } }, "id": "AHCKFKLKGB_661", "locus": "GCA013367975_00649" }, { "type": "cds", "sequence": "contig_1", "start": 375326, "stop": 375583, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTAAAAGATTTAGAGAATAAACGCAAAAGTTTAAACAATATTTCATTAAAAGTAAATAATGAAAAAAATATTTATTTAATAAATATTATGAAACTTAACCAAAGATTAAGCTTTTTTAATAAAAATGCATTGGTATCAAGGCAAATAATTTTGCATTTAAAAGAAATCTACAATATTAAGAATGATATGTTGAGGAGAGAAAGTCACCATAGGTTTAATTTATTTAGAAAAATAAATAGAAACATATTGTGA", "aa": "MQLKDLENKRKSLNNISLKVNNEKNIYLINIMKLNQRLSFFNKNALVSRQIILHLKEIYNIKNDMLRRESHHRFNLFRKINRNIL", "aa_hexdigest": "8f4cc15ed9b3f3fcb9f21307f0de9739", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10419.179999999986, "isoelectric_point": 10.83953685760498 }, "id": "AHCKFKLKGB_662", "locus": "GCA013367975_00650" }, { "type": "cds", "sequence": "contig_1", "start": 375585, "stop": 376088, "strand": "-", "frame": 2, "gene": null, "product": "DUF2714 domain-containing protein", "db_xrefs": [ "RefSeq:WP_036439154.1", "SO:0001217", "UniParc:UPI000513EE1C", "UniRef:UniRef100_A0A454C9U4", "UniRef:UniRef50_D1J8A5", "UniRef:UniRef90_D1J8A5" ], "nt": "ATGAAAAAGAAAGACAATAACCCATTATTGAGTTATTTTGATGTATTTAAAGAATATAAAGAACACGAGTCCTCACAAGATTTAATTACTTTTGATGAAATGATATCAACAGTATTATTTAACAACAACCTAGGATTTAATTCTCCTATTTATTTAGATTTTATAAGCAAAATTGATTTGGCTTTTAAAAATAAATATGACATTTTACTAAAGGATTTTAACATTACTTTTAATTTGAATTTGAAATTCTCACAAGAGGTATTAGTACCTATAATTACAACAAAAAATTATTCTGCCAATAACGCAATTGATTTAACTGCTTCTAGTGATATAAAATACCAAGAATTTTTGAATTCATATAATGCTGAAATAAAAAAACTTTTGGCCCTTAAAAAATCAATTAAGATATTTCCACATGTAATTTTATTTCATTCAGAATTAAAAAATGATTTAGTTTTATTATTTAGTGAAAAAGTAGTAGCAGTTGTTACAAAGGAACAATAA", "aa": "MKKKDNNPLLSYFDVFKEYKEHESSQDLITFDEMISTVLFNNNLGFNSPIYLDFISKIDLAFKNKYDILLKDFNITFNLNLKFSQEVLVPIITTKNYSANNAIDLTASSDIKYQEFLNSYNAEIKKLLALKKSIKIFPHVILFHSELKNDLVLLFSEKVVAVVTKEQ", "aa_hexdigest": "21fe0e74cd769d0538ceff5beddb570a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000513EE1C", "ncbi_nrp_id": "WP_036439154.1", "uniref100_id": "UniRef100_A0A454C9U4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513EE1C", "RefSeq:WP_036439154.1", "UniRef:UniRef100_A0A454C9U4" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9U4", "uniref90_id": "UniRef90_D1J8A5" }, "psc": { "uniref90_id": "UniRef90_D1J8A5", "product": "DUF2714 domain-containing protein", "uniref50_id": "UniRef50_D1J8A5" }, "pscc": { "uniref50_id": "UniRef50_D1J8A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8A5" ], "product": "DUF2714 domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_663", "locus": "GCA013367975_00651" }, { "type": "cds", "sequence": "contig_1", "start": 376069, "stop": 376500, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTAGCAATTGTTCAAATAGTGCTTGCGTTAAGTTTGCTATTGACTACATTATTTAACACCAATAGCATTGTTTCTTTAATCAACGTTGCTGTAACGGCAACAACACAAATAGTAATATTATTTGTGTTTGTTAAACAAAACAAGAATATTTCATTGCTATTATTAATAATAATTAAACTAGCAATAATCTCTTCAATTTTAATCTTTGCAATTATTGGATTTAATTATATTTTAACTGCTGATGGCAATGAAAATTATTTATTTTCAATAATTAAAAGTAGAATGAATATTATTCAAATTATTTTACTTTTAAACTTTAGCATTGCATTTATTATGGCATTATCTTTATTGCTTAAATTTATTTTGGCAATTTATTATATAAGAAAATTAAATAAGGAGAATGAATATGAAAAAGAAAGACAATAA", "aa": "MNLAIVQIVLALSLLLTTLFNTNSIVSLINVAVTATTQIVILFVFVKQNKNISLLLLIIIKLAIISSILIFAIIGFNYILTADGNENYLFSIIKSRMNIIQIILLLNFSIAFIMALSLLLKFILAIYYIRKLNKENEYEKERQ", "aa_hexdigest": "a70019bda0113a40c59d0350050772ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16237.546499999988, "isoelectric_point": 9.646098136901855 }, "id": "AHCKFKLKGB_664", "locus": "GCA013367975_00652" }, { "type": "cds", "sequence": "contig_1", "start": 376672, "stop": 376896, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGTAGGCAATAAATTTTATGAAACAATTAGAGATATTTTAACAATTAAATCTGCTAGCAATTTTGTGATTATTGTATTGACATTTTTAGTTTTAATTACTTTTATAGTATTATCTAATATTAAATTCTTTGCTTTATTGATAAATAAAAAATATGATAAGTTCTATTTAAAAAATCAATTACTATTTTTATTAGTATTATTTGGGGCTGTATTTATTTGA", "aa": "MFVGNKFYETIRDILTIKSASNFVIIVLTFLVLITFIVLSNIKFFALLINKKYDKFYLKNQLLFLLVLFGAVFI", "aa_hexdigest": "743c1e88d16c38a31320c8d39d0fcea8", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8658.491099999996, "isoelectric_point": 9.822999382019042 }, "id": "AHCKFKLKGB_665", "locus": "GCA013367975_00653" }, { "type": "cds", "sequence": "contig_1", "start": 376924, "stop": 377166, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTCATTAACTTCTTATACATTATTTTTTAGCTATTTTAAAATTCTTCCAAGCAAATTATTTGCAATATCATTTATTTTGATTGCTTTATATATTATTAATTTTAGCTATGAAATATTTAACTTTTTAATTCAAAGAAAAACAAATCCATTACTTTATAAAAATAAAAATATTTTAATAATTTCTTTAACATTTCAAGGAATCTTAACAATATTATTTGTTGCAATATCATTTGCTTGA", "aa": "MLSLTSYTLFFSYFKILPSKLFAISFILIALYIINFSYEIFNFLIQRKTNPLLYKNKNILIISLTFQGILTILFVAISFA", "aa_hexdigest": "ae156c7096a307d089c8ed38e5ae3523", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9342.196699999991, "isoelectric_point": 9.822999382019042 }, "id": "AHCKFKLKGB_666", "locus": "GCA013367975_00654" }, { "type": "cds", "sequence": "contig_1", "start": 377215, "stop": 377550, "strand": "-", "frame": 1, "gene": null, "product": "MSC_0624 family F1-like ATPase-associated membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E41E527", "UniRef:UniRef90_UPI001E41E527" ], "nt": "ATGAAACAAAAACAAATCGATTCGAATAATATAAATCAAAAATTGATTTATGATGATGAATATTCAATAAAAAATCAGCAGAAAAACAACATTTTCAATAAGGTGTTGAAATACCTTTTGATTGTAATTAGTCTTGTATTTATTACAACTTTATTAATTTTTAGTGAAAAGACACTTTTTGCTAATAAAATGTTGCTTTCTAATTCACTAAATTCATTTTTTGACTTTTCAAGTCCAAGAAATCAACAATTAAACTTTTTAATAATTTATAGATTCTTCATTTTAGCCTTTGTCTTATTTTATAGTTTAGTAAAAGGTTTTGCTAATTTATATTGA", "aa": "MKQKQIDSNNINQKLIYDDEYSIKNQQKNNIFNKVLKYLLIVISLVFITTLLIFSEKTLFANKMLLSNSLNSFFDFSSPRNQQLNFLIIYRFFILAFVLFYSLVKGFANLY", "aa_hexdigest": "a10b32784efe381e88fa4d0edaf3f013", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 376912, "stop": 377550, "observations": { "mutation": [ 376926, 377217 ], "stop": [ 376926, 377217 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E41E527", "query_cov": 1.0, "subject_cov": 0.4036363636363636, "identity": 0.982, "score": 197.0, "evalue": 2.58e-61, "gene_start": 1, "gene_end": 111, "reference_start": 1, "reference_end": 111, "reference_sequence": "MKQKQIDSNNINQKLIYDDEYSIKNQQKNNILNKVLKYLLIVISLVFITTLLIFSEKTLFANKMLLSNSLNSFFDFSSPRNQQLNFLIIYRFFILSFVLFYSLVKGFANLYWHKVTIKKYIPFYFLYLLLSLTSYTLFFSYFKILPSKLFAISFILIALYIINFSYEIFNFLIQRKTNPLLYKNKNILIISLTFQGILTILFVAISFVWINLGINKDILFVGNKFYETIRDILTIKSASNFVIIVLTFLVLITFIVLSNIKFFALLINKNMINSI" }, "paralog": false, "identity": 0.9764150943396226, "score": 398.0, "evalue": 4.37e-143, "description": "Internal stop codon at 376926, 377217. Nonsense mutation around 376926, 377217.", "psc": { "uniref90_id": "UniRef90_UPI001E41E527", "product": "MSC_0624 family F1-like ATPase-associated membrane protein", "uniref50_id": "UniRef50_UPI001E41E527", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E41E527", "UniRef:UniRef50_UPI001E41E527" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E41E527", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E41E527" ], "product": "MSC_0624 family F1-like ATPase-associated membrane protein" } }, "id": "AHCKFKLKGB_667", "locus": "GCA013367975_00655" }, { "type": "cds", "sequence": "contig_1", "start": 377566, "stop": 377913, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAGAAGACTTACTTTATATAATAACAGTGAAGCGTTCAACATATCAGGTGAAGAATTAAATGAAGCAGGCTTTAATAGACATATTGTTTCAGGCGAACCTGGTCAAAAAAGCAAGATTTTATTTAGTAATGGAAATTTAACAACAAAAGTAAGAATCACAGGAACTTCATTACTAAATTCAGAAGGAATTGACAACCTTTCGCGTTTATATATGTTTGCAGAACAATTGAAAAAAGAAATAATTGTTGATAATAATGCCTCAGCTTTAAAATCTCAATTAGAAGGTTTGGGATATAGTGTAAAGACCTCTTCTTCTTCTGATTCTGACGGAGAATTTATTTAA", "aa": "MIRRLTLYNNSEAFNISGEELNEAGFNRHIVSGEPGQKSKILFSNGNLTTKVRITGTSLLNSEGIDNLSRLYMFAEQLKKEIIVDNNASALKSQLEGLGYSVKTSSSSDSDGEFI", "aa_hexdigest": "fe26518889725d39460cec536a931f09", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12620.94469999999, "isoelectric_point": 5.386767768859863 }, "id": "AHCKFKLKGB_668", "locus": "GCA013367975_00656" }, { "type": "cds", "sequence": "contig_1", "start": 378298, "stop": 378483, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAATCTGACTCTAGCCAAAAATTTATGGATATTTTAAAGGCAATTCCTGATAAAATTCAACTACTAGAACTATTTTTCTCAGCAGGTTCTTCAAACACTTCTTCTTTAATTGCATTAGAAAATAAACACATAAAGGAACTAGGAATTTATACTTTAGGAAATTCATTATTGGATAAATGA", "aa": "MGKSDSSQKFMDILKAIPDKIQLLELFFSAGSSNTSSLIALENKHIKELGIYTLGNSLLDK", "aa_hexdigest": "4f1f6c20bb49da91bc8d8797edc840a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6700.684299999998, "isoelectric_point": 6.533323860168457 }, "id": "AHCKFKLKGB_669", "locus": "GCA013367975_00657" }, { "type": "cds", "sequence": "contig_1", "start": 378709, "stop": 378975, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCTCATTTAGATTTCTCTAAATTTACAAAGATTACTTCTAATGCTGAAAAGCAATTAGCTGAAGGCCTTGCATTAGATCAAGATAACGCCTATGTAAATGAAAAAGGTGAGCTAGATTCATATGCATATTCTCCAGCCGAAGGCTATAACGGTGTTGCAAATAGAATAATAAATGACAATAAAAATAGAAGAGTTTTTGGATATGATAGTATTGAAAATAGAAGCCCATATGGTCTTGCTAATGGCGACTATCCTGGTTGA", "aa": "MYSHLDFSKFTKITSNAEKQLAEGLALDQDNAYVNEKGELDSYAYSPAEGYNGVANRIINDNKNRRVFGYDSIENRSPYGLANGDYPG", "aa_hexdigest": "a1bcc7199b4045fa88d9b8f45cadf358", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9833.539199999996, "isoelectric_point": 4.780182075500488 }, "id": "AHCKFKLKGB_670", "locus": "GCA013367975_00658" }, { "type": "cds", "sequence": "contig_1", "start": 379183, "stop": 379866, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACTTATTAAAGAAAAGAAAAAACAAATTAATTTTAATGACGGCAGGTGTTATTATAGGAGCTTCAATTATTGCTGGGGCTATATTTTATAGTACAAGTTCGCAAAATTATTCTCGTTTTAATACCACCAGCTCAGCAACAGGAAATGATTTTATAAATAAAAGCAACCTAGATTATAAAAATGCGTTGCCTTCAATAAAGGATAACAACTTAAAAGAATTAGAAAAGCCACAACCAATTCCAACCCCTAAACCAGAACCTGAACCAATTCCGGTTCCACCGGCTCCAAAACCTAAACCAGAGCCCGAACCCAAACCAGAGCCAAAACCTCAACCTCAACCAACGCCTCCAACTCCCAAACCAGAGCCAAAACCCAAACCCGAACCCAAGCCTACTCCTCCTTCATCAAAACCTTCAACTAGAACAACTAAAATTATTCTAAACGGTGTTGAAGTTGAAGCGGAAGTTGAAGATCAAAAGAAACGTGAATATAATCAATATGATATCTTAAATCAACTAACTAATTTAAACCCATATATCAATGATAGTGCTCCAAAAATTAAAAAAGTTAAGGTTACTGAAGAATTGAAACAAGCAGCAGCCAAAGCGGCTACTCAACGTTTAAAAAGAGATGAAAAAAACTTCAAAAAAGATCTTGAAGATTATGAAAAAAGTTGA", "aa": "MKNLLKKRKNKLILMTAGVIIGASIIAGAIFYSTSSQNYSRFNTTSSATGNDFINKSNLDYKNALPSIKDNNLKELEKPQPIPTPKPEPEPIPVPPAPKPKPEPEPKPEPKPQPQPTPPTPKPEPKPKPEPKPTPPSSKPSTRTTKIILNGVEVEAEVEDQKKREYNQYDILNQLTNLNPYINDSAPKIKKVKVTEELKQAAAKAATQRLKRDEKNFKKDLEDYEKS", "aa_hexdigest": "0dc22bc0cbca314aef25f88df67ed68e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25391.811700000013, "isoelectric_point": 9.56325626373291 }, "id": "AHCKFKLKGB_671", "locus": "GCA013367975_00659" }, { "type": "cds", "sequence": "contig_1", "start": 379897, "stop": 380346, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGAAGCAAGTTCAAGAAGAGAAAGATGGCCAAAAATATCAAAAATCAGTATTTTCGTTCTATGAAAGTAGCGATGGCAAAACATATATGCGTATGGGACTAAATTATATGCCTAAACATTTTGCTCCAGTTGGCGGCGCTTCTGGTACAAGCTTAAGAAATCAAAAAAACGAACTTGTTGGAATATTTTATTTATCGAATTTAGGTGCAAAAACCGGCTTAGCAGCTGCTTTTAGATCGGAAGGATTTGACTACGAAGGCTTGTATGGAAATTACAATTTGCCTCAATATGATCTAATTTACGGTGGTGGTAAAGACCAAAAAAATTCATTTAGACAAACATTAAAAGAACTATATAAAAATAATAGCGATTTTAAAACAGCATTATTCCCTGATGGCTTAGGTGATAATGAAGTTCCTAATGAATTTAAATTTATAGAAAAAAAATAA", "aa": "MKQVQEEKDGQKYQKSVFSFYESSDGKTYMRMGLNYMPKHFAPVGGASGTSLRNQKNELVGIFYLSNLGAKTGLAAAFRSEGFDYEGLYGNYNLPQYDLIYGGGKDQKNSFRQTLKELYKNNSDFKTALFPDGLGDNEVPNEFKFIEKK", "aa_hexdigest": "2be5eb4baa381a7b46cc937896ed3291", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 57, "aa_cov": 0.3825503355704698, "hmm_cov": 1.0, "evalue": 8.633727000715887e-14, "score": 47.19933319091797, "start": 8, "stop": 64 } ], "seq_stats": { "molecular_weight": 16896.71909999999, "isoelectric_point": 8.596810722351076 }, "id": "AHCKFKLKGB_672", "locus": "GCA013367975_00660" }, { "type": "cds", "sequence": "contig_1", "start": 380641, "stop": 381096, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGGATAGTGCAAAAATTAGAACAACATTGCGTTTGGCAAATCTTGATGAAAATACATATAGATTTTCATTTGTTGGTAAAGATTCTACTAATGTTTCTAATGGAATAAAGACAGTTTATGCTGCAAGAGATTTCTTAAATTCAAAACCTTCTGATTATTTAATTAAATCGCAAAAAGAAAAGTATAAAGATGTTGAAGAATATGCTGATTTTGCAGTTTTTGAAGTTGATTTTGAAAAATTAACATTACAAAGCGTTTCTAAAAATGATCTAAGCGAAAATAGCGATATTACTAAAAACTTTAGCAATTTAGATGCTCACGAATTAGCAAGAGCCTTAACAAGTAATTATGCTCAAAATAAAGAAAAACAAATTAAATTCTTGTCGAAATCATATTTATCAAATTATCAAAAAATTGATGTTCCTTTAAAAGCAAATAAAGAAAACAAAGAATGA", "aa": "MDSAKIRTTLRLANLDENTYRFSFVGKDSTNVSNGIKTVYAARDFLNSKPSDYLIKSQKEKYKDVEEYADFAVFEVDFEKLTLQSVSKNDLSENSDITKNFSNLDAHELARALTSNYAQNKEKQIKFLSKSYLSNYQKIDVPLKANKENKE", "aa_hexdigest": "651a3a4c4830c0bda9bde7bd153cd19e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 149, "aa_cov": 0.9271523178807947, "hmm_cov": 0.8322147651006712, "evalue": 9.040612556951932e-26, "score": 86.59517669677734, "start": 2, "stop": 141 } ], "seq_stats": { "molecular_weight": 17373.1719, "isoelectric_point": 7.785991859436035 }, "id": "AHCKFKLKGB_673", "locus": "GCA013367975_00661" }, { "type": "cds", "sequence": "contig_1", "start": 381217, "stop": 381825, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTTAATATTACTATTAAAGACAAAAAGACAAACAAAACATTTATTGAAACAATAACTGTAGGAAAATTTAAAACAAATCCTTATGGTTTTGATGAAAATGGAACAATTCCTAATACAGGTTCTGATGCTTTAAGATTGAATAAAAGTGAGTTAGAACAATATCTTGAAGGAAATCAAACAAAAAGATATGAGCTAGATAATAAAAAATATTTAGACATTTTAACTCGTCAACAAAAACAAAAATCAATAAAAGAAATTAGACCCAATTTGGAAGCTTCACAATCTAACATTCAAAAATTTGATGCCAAAGCAAAAGAAATTAGTCAAGATTCGTATGAATCAGCTGCAAGAAAAGGTTTTACATTGCCAAGCTACGATTCAAATGGCAAAGTAGAAGGCCTTGCTATTAATGAAAATCCAGGAGGGCAAGGACCTTCACCTACAGATGCACTTGATAGAGATCCAAATAAAATTACAGGTTTAGCAAGAACATTGCCAAATGAACATTACCGTGACATTGCTTCACAAACATTTGGAATAAGATTCTTAACTCCTATTGAAGGCGAAGAAAGAAAATTTACTAATACTGCTGGAACAACTTGA", "aa": "MNFNITIKDKKTNKTFIETITVGKFKTNPYGFDENGTIPNTGSDALRLNKSELEQYLEGNQTKRYELDNKKYLDILTRQQKQKSIKEIRPNLEASQSNIQKFDAKAKEISQDSYESAARKGFTLPSYDSNGKVEGLAINENPGGQGPSPTDALDRDPNKITGLARTLPNEHYRDIASQTFGIRFLTPIEGEERKFTNTAGTT", "aa_hexdigest": "f257e0d64ca830d071ff5130701d23d6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22701.955599999994, "isoelectric_point": 8.596037101745608 }, "id": "AHCKFKLKGB_674", "locus": "GCA013367975_00662" }, { "type": "cds", "sequence": "contig_1", "start": 381850, "stop": 382320, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAATCTAAATTAATAATATCATTATTAATAGCAGCGACATCACTTTCTAGTATGTCTGCTGTAGCTATTTCATGTAAAAATACAAATAAAAAGACTAATAATAATCAAAATGACGAAAAAAAGAATCCTGATGAAAAACCCCAAAATAATCAAGATTCAAAAGGCGACGACAACAAAAATAATAATTCTACAGATGAACTATCAGCTGAAGAACAAAAAACTACCGAATATAAAAAAATAATTTCTGAAGTTTCAAAATTAGCGTTAAATGATCCAAGTCAATTATTTGATTTGAAATTCAAAGCTTTTTCAAATCAAAATTCACAAGATTTGCTTCCATCTCAAATTGTTAGAAATTTCAATCAAGGTGTTGAATTTAAACAAAAAAAATTTAATGATGAAATTGAAATTAAAAAAATAGTCAATGTTTTATATGTACAAACTCCAACCAATTCTGATGATTGA", "aa": "MKKSKLIISLLIAATSLSSMSAVAISCKNTNKKTNNNQNDEKKNPDEKPQNNQDSKGDDNKNNNSTDELSAEEQKTTEYKKIISEVSKLALNDPSQLFDLKFKAFSNQNSQDLLPSQIVRNFNQGVEFKQKKFNDEIEIKKIVNVLYVQTPTNSDD", "aa_hexdigest": "d95955f972cb05270f8c02f1d7e0eb02", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17616.4337, "isoelectric_point": 7.710970878601075 }, "id": "AHCKFKLKGB_675", "locus": "GCA013367975_00663" }, { "type": "cds", "sequence": "contig_1", "start": 382634, "stop": 383020, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002614D0AA" ], "nt": "ATGTTGGATGTTTGCAATTTAGCTAAAGTTTTCCCAGACAAAAAATTATTTACAAATGTTAACTTAAAATTCTTGCCTGGAAATGTATATGGAATTATTGGCGCTAATGGGGTTGGAAAATCCACTTTTTTAAAAATATTGAGTGGGGATATTGAAGCATCAGAAGGGCAAATAATTAAAGAAAAAAATGCTAGAATGAGTGTATTATCACAAAACCAAAATGAATTCGACGATTATATTGCAACTGATGTTGTAATAATGGGAAATAAAGAACTTTATCAAATCAATCTTGAAAAAACTAAAATTTATGAAGATCCAAATGCAACTATTCAAGATTATGAAAGAGCAAGCCATTTAGAAGAAAAATTTGGTGAAATGGGGGGTTGA", "aa": "MLDVCNLAKVFPDKKLFTNVNLKFLPGNVYGIIGANGVGKSTFLKILSGDIEASEGQIIKEKNARMSVLSQNQNEFDDYIATDVVIMGNKELYQINLEKTKIYEDPNATIQDYERASHLEEKFGEMGG", "aa_hexdigest": "4b35b0f957e60a343fa0f2a34b04df18", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002614D0AA", "query_cov": 1.0, "subject_cov": 0.9343065693430657, "identity": 0.5, "score": 124.0, "evalue": 3.77e-34, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI002614D0AA" }, "pscc": { "uniref50_id": "UniRef50_UPI002614D0AA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002614D0AA" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_676", "locus": "GCA013367975_00664" }, { "type": "cds", "sequence": "contig_1", "start": 383084, "stop": 383212, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGTCGGATTTAAAAGCTAATGAAAAAGTAAAGGTTTTATTGGCAAAGGCTTTGTTTGGAAATCCCGATATTTTAGTAATGGATGAACCAACAAACAGATTGGATCTAAAAACAATTAAGTGA", "aa": "MKMSDLKANEKVKVLLAKALFGNPDILVMDEPTNRLDLKTIK", "aa_hexdigest": "767e909f7a3a553f1973341b68c98a0f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4742.6662, "isoelectric_point": 9.22402362823486 }, "id": "AHCKFKLKGB_677", "locus": "GCA013367975_00665" }, { "type": "cds", "sequence": "contig_1", "start": 383228, "stop": 383353, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTATCCAAACATTGTTATTGTTGTTTCTCACGATAGTGATTTCTTAGATGCAATTTGCACGCATATTATTGATATAGATTACTCTGAAGCTAAATTATTTATTGGAAATTATTCATTTTGA", "aa": "MDYPNIVIVVSHDSDFLDAICTHIIDIDYSEAKLFIGNYSF", "aa_hexdigest": "459d1e9fe7647d18e60ab69fb0c47a61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4695.236499999998, "isoelectric_point": 4.0500284194946286 }, "id": "AHCKFKLKGB_678", "locus": "GCA013367975_00666" }, { "type": "cds", "sequence": "contig_1", "start": 383381, "stop": 383770, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33", "PFAM:PF12848.13" ], "nt": "ATGCAACAAAAACAAAACCTTAAAAAAGAAGAAATTGCCCAAAAATTAAAAGATTTTATTGCCAGATTCTCTGCTAATGCCTCAAAATCAAAACAAGCCACAAGTAGAAAGAAATTACTTGATAAAATTGTCATTGATGAAATCAAACCATCATCTAGAAAATATCCTTTCATTCAATTTAACTTAAATAGAACTCCTGGAAAACAAATATTAACTGTTAAAAATTTAACTTATGTAAATGACAATGGCGACACTTTATTTGAAAATGTAAGTTTTACAATCCACCCTGGAGAAAAAATGGTTATTATTGGCAATGACGACATTGCCAAAACTAGATTACTTGAAATACTTATGGGCAAGCGTAAACCAACATCAGGAACAGTTGAATGA", "aa": "MQQKQNLKKEEIAQKLKDFIARFSANASKSKQATSRKKLLDKIVIDEIKPSSRKYPFIQFNLNRTPGKQILTVKNLTYVNDNGDTLFENVSFTIHPGEKMVIIGNDDIAKTRLLEILMGKRKPTSGTVE", "aa_hexdigest": "a837073c1b585038c050706acd779c73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 41, "aa_cov": 0.3178294573643411, "hmm_cov": 1.0, "evalue": 6.33418460786387e-08, "score": 28.639638900756836, "start": 88, "stop": 128 }, { "id": "PF12848.13", "name": "ABC_tran_Xtn", "length": 59, "aa_cov": 0.4573643410852713, "hmm_cov": 0.9661016949152542, "evalue": 3.982071503480089e-13, "score": 44.65205001831055, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 14693.900499999978, "isoelectric_point": 9.908742332458495 }, "id": "AHCKFKLKGB_679", "locus": "GCA013367975_00667" }, { "type": "cds", "sequence": "contig_1", "start": 383909, "stop": 384247, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGCATTTTTAGGAAGAATGTTATTTTCAAATGATAGCGTATTTAAAAATGTTAATGTAACTAGCGGTGGTGAAAAAGTTAGATTAATGATGGCTAAACTAATGTTAGAAGAAAGTAACTTTTTAATCTTTGACCAACCTCTTGACCACCTTGATAGTGAATCTATTGATAGTTTAATTGAAGCAATTAAAAATTACAAAAGTAGTTGTATATTCACAACATACAATAGGGCAATGATAAAAGAATGCGCCTCAGTTATTTTAGAAATAAAGCCTACTTCAAGTTATTTATTCTATGGAACCTTAGAAGAATATGAAGAAGCAATGGGATATTAA", "aa": "MRAFLGRMLFSNDSVFKNVNVTSGGEKVRLMMAKLMLEESNFLIFDQPLDHLDSESIDSLIEAIKNYKSSCIFTTYNRAMIKECASVILEIKPTSSYLFYGTLEEYEEAMGY", "aa_hexdigest": "09499d814b522967d8a94203f3f165be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12834.661299999987, "isoelectric_point": 4.819230461120605 }, "id": "AHCKFKLKGB_680", "locus": "GCA013367975_00668" }, { "type": "cds", "sequence": "contig_1", "start": 384774, "stop": 385127, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGTTTTTTAATTTAAAAAATCTTGAAAAAAGATTTATGGAATTGCAATATGGGGCAGGCATACCGGCTATTGACTTAAAAAAATTAAAAAATATTCAAATTCCACTTCCCCCTCTTGAAATCCAAAATCAAATTGTTAATATTTTAGACAAGTTCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCTATAGAAATAAACAATATAATTACTATAGAAATAAATTACTAGACTTTGATAATAATAAAGAATTATTAAACAAGATAATGAATAATCAACAATGCAATAATAGTATAGTTGAATATAAAAAATTAGAAGAAGTAGTTGACTATTTACAACCTACTAAATGA", "aa": "MFFNLKNLEKRFMELQYGAGIPAIDLKKLKNIQIPLPPLEIQNQIVNILDKFTELTTELTTELTYRNKQYNYYRNKLLDFDNNKELLNKIMNNQQCNNSIVEYKKLEEVVDYLQPTK", "aa_hexdigest": "1e2f24ec51b981aa87bf341fe7bdcf23", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 62, "aa_cov": 0.5299145299145299, "hmm_cov": 0.9838709677419355, "evalue": 8.696522664120982e-15, "score": 50.63947296142578, "start": 2, "stop": 63 } ], "seq_stats": { "molecular_weight": 14006.034799999983, "isoelectric_point": 7.737912559509279 }, "id": "AHCKFKLKGB_681", "locus": "GCA013367975_00669" }, { "type": "cds", "sequence": "contig_1", "start": 385275, "stop": 385448, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAATAGAAAAGTTAATTAAGGAACTATGTCCTAATGGAGTTGAATATAAGAGCCTAGATGGCTTATGTGAAATAAAAAAAGGTCAACAATTAAACAAAGAAAATTTTAAATCTATTGATGGGGGATATCCTGTTATTAATGGAGGCCAGGCACCGAGTGGTTATTGA", "aa": "MNKIEKLIKELCPNGVEYKSLDGLCEIKKGQQLNKENFKSIDGGYPVINGGQAPSGY", "aa_hexdigest": "43312ac62d7512e39d36ca9dddcb17ee", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6258.1181000000015, "isoelectric_point": 7.729102516174316 }, "id": "AHCKFKLKGB_682", "locus": "GCA013367975_00670" }, { "type": "cds", "sequence": "contig_1", "start": 385451, "stop": 385915, "strand": "-", "frame": 3, "gene": null, "product": "type I restriction-modification system subunit M", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0009307", "KEGG:K03427", "SO:0001217", "UniRef:UniRef50_A0A7X6YKI2", "UniRef:UniRef90_UPI001939D1E0" ], "nt": "ATGTATCGTGGGGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAAGCAACTTGTTTTTTGGAACAAGTATTTCAACATGCATAATGGTATTAAAAAAATCAAAAATTGACAACAATATCTTATTTATTGACGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAACAAGCTAACTAGTCAAAATATTAACAATATTATTGATTATTACAGCCAAAGAAAAGACATCTCTTATATTTCAAAATTAGCCAGTGTTGAAGACATTAAGTCCAATTCATATAATTTATCAGTTAATAGTTACGTTGAAAAACAAGACACTTCAGAAAAAATAGATATCAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAACATTGAGAAGTGAAATTGACAAAATAGTTGCCGAATTAGAGGAATAG", "aa": "MYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYSQRKDISYISKLASVEDIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE", "aa_hexdigest": "c651805964eb275b8a85b0613fec3ec3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_R6V7S9", "query_cov": 0.9935064935064936, "subject_cov": 0.9807692307692307, "identity": 0.654, "score": 197.0, "evalue": 1.73e-62, "valid": false, "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A645HPD1" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 385451, "stop": 386215, "observations": { "mutation": [ 385963, 386074 ], "stop": [ 385963, 386074 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939D1E0", "query_cov": 1.0, "subject_cov": 0.4652567975830816, "identity": 0.981, "score": 277.0, "evalue": 2.46e-91, "gene_start": 1, "gene_end": 154, "reference_start": 178, "reference_end": 331, "reference_sequence": "MYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIKWEGEDNPLLINDQRFSPAGILAPKSKADFAFILHSLSWLATDGVAAIVCFPGIMYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQWKDISYISKLASVEEIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE" }, "paralog": false, "identity": 0.9763779527559056, "score": 484.0, "evalue": 2.02e-175, "description": "Internal stop codon at 385963, 386074. Nonsense mutation around 385963, 386074.", "psc": { "uniref90_id": "UniRef90_UPI001939D1E0", "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7X6YKI2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939D1E0", "EC:2.1.1.72", "UniRef:UniRef50_A0A7X6YKI2", "KEGG:K03427", "GO:0003677", "GO:0009007", "GO:0009307" ] } }, "id": "AHCKFKLKGB_683", "locus": "GCA013367975_00671" }, { "type": "cds", "sequence": "contig_1", "start": 386072, "stop": 386899, "strand": "-", "frame": 3, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "nt": "ATGTTTTATAGATATATTTCCGAAAATATTGCAACATATGCAAACAATCGCCAACGTCAAGCAGGAATTGAAGACTTTGATTATACAACTTTATCAGATGAAGAAGCATTGACTGGAAGAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTGCCTTCTGAATTGTTTATTAATGTTGTTAGAAATGCTACAACAAATAATTGTTTAAATGAAACATTGGACAATATTTTTAAAAATATTGAATCATCAGCAAAAGGACAACAAAGTGAAAATGATTTTTCAGGTTTATTTAATGACGTTGATGTTAATTCTCAAAAACTAGGTCGTAGTGTCGATGAAAGAAATAAAAAATTAGCAGCAATATTGCAAGAAATAGCAGCCATGAAATTAGGCAATTACCAAGATAATTCAATAGATGCCTTTGGAGATGCTTATGAATATTTGATGTCAATGTATGCATCAAATGCTGGAAAATCAGGGGGTGAATATTTTACTCCTCAAGAAGTTTCAGAATTACTTACTAAAATAGCAGTTTTCAATAAGAAAAAAGTTAATAGAGTGTATGACCCAGCATGTGGATCGGGTTCTTTACTTTTACAAACAATCAAGGTTCTTGGCAAAGAAAACATCAAGGATGGATTTTATGGCCAAGAAGTCAATTTAACAACATATAACTTATGTAGAATAAATATGTTTTTGCATGATATAGGATTTGATAAATTCAATATTTACAACGGCGACACATTGCTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTTATTGTTTCAAATCCTCCTTATTCAATTAAATGA", "aa": "MFYRYISENIATYANNRQRQAGIEDFDYTTLSDEEALTGRDDLINEKGFFILPSELFINVVRNATTNNCLNETLDNIFKNIESSAKGQQSENDFSGLFNDVDVNSQKLGRSVDERNKKLAAILQEIAAMKLGNYQDNSIDAFGDAYEYLMSMYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIK", "aa_hexdigest": "84a04d1b79b4b94b47e206e06b4a5ce5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0028EAAB7E", "query_cov": 1.0, "subject_cov": 0.8136094674556213, "identity": 0.662, "score": 368.0, "evalue": 3.84e-125, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7U9ST90", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9ST90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "AHCKFKLKGB_684", "locus": "GCA013367975_00672" }, { "type": "cds", "sequence": "contig_1", "start": 387206, "stop": 387760, "strand": "-", "frame": 3, "gene": "yajL", "product": "DJ-1/PfpI domain-containing protein", "db_xrefs": [ "COG:COG0693", "COG:V", "RefSeq:WP_175933496.1", "SO:0001217", "UniParc:UPI00158C2F9B", "UniRef:UniRef100_UPI00158C2F9B", "UniRef:UniRef50_D1J8B4", "UniRef:UniRef90_D1J8B4" ], "nt": "ATGAATTTATTAGTAATTGTTGAAGAAGGATTTGAAGATACTGAATTAACAGGAACATTAGATTTATTTCATAGAGCAAATAATTTATTTAATAAGACCACATTTTACAATCCTCTAGGAATTGAAGCGACTGGTGCACATAAAATTGTAAAATTGCAAACAACTTCAAATATAAATGTTGATGAATATGATGTTTTATTTATTCCTGGAGGACCAGCTGCTAAAAGTATTAGAAAGAACCAAACATTTTTAAACTTAATAGCTAAATTCAAAAGTCAAAACAAATTAATTATAGCAATATGCGATGCTCCCAATGTTTTATATGAAAATAAGTTTATTGATAGTAAAACTAAATTTAGTTCATTTCCAATAAACGACAGTGTAAAAGAAATATCTAATCGTAGTAAAGAATTAGTATCAATAAGCCCAAATAAAAAATTAATAACAGGAAAATGTGCGGCTGCTGTTTTTGATTTTGCATTAGCTGTTATTGAAGTTTTGGGTGGCAATCAACTTGCTAAATTAGTTCTTCAAGCAATAAAACCTATTGATTAA", "aa": "MNLLVIVEEGFEDTELTGTLDLFHRANNLFNKTTFYNPLGIEATGAHKIVKLQTTSNINVDEYDVLFIPGGPAAKSIRKNQTFLNLIAKFKSQNKLIIAICDAPNVLYENKFIDSKTKFSSFPINDSVKEISNRSKELVSISPNKKLITGKCAAAVFDFALAVIEVLGGNQLAKLVLQAIKPID", "aa_hexdigest": "a09eec80515e81a0089be7ed2dbc523b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00158C2F9B", "ncbi_nrp_id": "WP_175933496.1", "uniref100_id": "UniRef100_UPI00158C2F9B", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C2F9B", "RefSeq:WP_175933496.1", "UniRef:UniRef100_UPI00158C2F9B" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C2F9B", "uniref90_id": "UniRef90_D1J8B4" }, "psc": { "uniref90_id": "UniRef90_D1J8B4", "gene": "YajL", "product": "DJ-1/PfpI domain-containing protein", "uniref50_id": "UniRef50_D1J8B4", "cog_id": "COG0693", "cog_category": "V" }, "pscc": { "uniref50_id": "UniRef50_D1J8B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B4" ], "product": "DJ-1/PfpI domain-containing protein" }, "genes": [ "yajL" ], "id": "AHCKFKLKGB_685", "locus": "GCA013367975_00673" }, { "type": "cds", "sequence": "contig_1", "start": 387913, "stop": 388092, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF", "UniRef:UniRef90_UPI0015F0C8A9" ], "nt": "ATGCCTGGTTTATATATTAATATTCATAAATTAACAATTCATATGAATTCAAATCCTAAAACAATTGAAATGGATTTTAGCTTAAAATATTCAAAACCTAAAGAACATCAACAAAAGATTGAAGGTTTTGAAGATGACTATATTTTTGAATATAAATATATATTCAAAGAAAAAAAGTAA", "aa": "MPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKPKEHQQKIEGFEDDYIFEYKYIFKEKK", "aa_hexdigest": "bb4655e076c067ee84989a364753b6ae", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 387913, "stop": 388260, "observations": { "mutation": [ 388101, 388185 ], "stop": [ 388101, 388185 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "query_cov": 1.0, "subject_cov": 0.5086206896551724, "identity": 0.966, "score": 118.0, "evalue": 2.44e-33, "gene_start": 1, "gene_end": 59, "reference_start": 58, "reference_end": 116, "reference_sequence": "MFEAKFIDNENVDLKEAFFKYNSGYSNFKKTKVYANLKSGEITIKNFELGAAKDWKELPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK" }, "paralog": false, "identity": 0.896551724137931, "score": 209.0, "evalue": 1.13e-71, "description": "Internal stop codon at 388101, 388185. Nonsense mutation around 388101, 388185.", "psc": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0015F0C8A9", "UniRef:UniRef50_UPI001939F8FF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7228.2817, "isoelectric_point": 7.9542543411254885 }, "id": "AHCKFKLKGB_686", "locus": "GCA013367975_00674" }, { "type": "cds", "sequence": "contig_1", "start": 388183, "stop": 388326, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D981E8", "UniRef:UniRef90_UPI0034D981E8" ], "nt": "TTGCTTCTAGAAGATAAAGAATCCAATTCAAAAAAATATATTTTTTTCTTGATTCCTAAAAAAGTTTTACTCGAAGCAAAATTTATTGATAATGAAAAAGTTGATTTAAAAGAAGCATTCTTTAAATATAATTCATTATATTGA", "aa": "MLLEDKESNSKKYIFFLIPKKVLLEAKFIDNEKVDLKEAFFKYNSLY", "aa_hexdigest": "fd198994e37ddfc9c250bd614ca24319", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 388189, "stop": 388440, "observations": { "mutation": [ 388341, 388353, 388365, 388410 ], "stop": [ 388341, 388353, 388365, 388410 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D981E8", "query_cov": 0.9361702127659575, "subject_cov": 0.5301204819277109, "identity": 0.977, "score": 86.3, "evalue": 3.61e-21, "gene_start": 2, "gene_end": 45, "reference_start": 40, "reference_end": 83, "reference_sequence": "MQSNKSIAGPWRNDFLEIAIGPKSKWFEYWKDYWILDRFVLEDKESNSKKYIFFLIPKKVLLEAKFIDNEKVDLKEAFFKYNS" }, "paralog": false, "identity": 0.891566265060241, "score": 143.0, "evalue": 2.94e-46, "description": "Internal stop codon at 388341, 388353, 388365, 388410. Nonsense mutation around 388341, 388353, 388365, 388410.", "psc": { "uniref90_id": "UniRef90_UPI0034D981E8", "uniref50_id": "UniRef50_UPI0034D981E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D981E8", "UniRef:UniRef50_UPI0034D981E8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D981E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D981E8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5691.6315, "isoelectric_point": 7.943101310729982 }, "id": "AHCKFKLKGB_687", "locus": "GCA013367975_00675" }, { "type": "cds", "sequence": "contig_1", "start": 388914, "stop": 389339, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B6", "UniRef:UniRef90_UPI00158BFF58" ], "nt": "TTGCCAATATTGCCCTTATTTGTAACCTCTTGCAATAATAGAGAAACTGAATTAAAAAATGCCGAAACAATTATTGGCAATAAAGAAATAGTTTTTAATATGGTTGATATTTTAAATAGCGAAGAAGAATCAGTTTTAAATAACGACGCAACTATTTCTGGCCCATGGCGTAATGATTTTTTTAAAATTGTAATAACATCAAAAAAGAATTTTGATAAATATGAAAAAGATTATTGTGTTTTTGGAAAGATAGATTTTTATGCATATTCCATTTTGTTTTTAATTCCTAAAAAGGTATTATTAGAAACAGGATTTATTTCAAGCGAAGATTTTAATTTAGCAGACTTTTATAGTAAAAACCTTAATTTCGTGTTTAATGAACAACCTAGAAAAATTTCGACAAATTTAAAAAATAAAAAAATATGA", "aa": "MPILPLFVTSCNNRETELKNAETIIGNKEIVFNMVDILNSEEESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSKNLNFVFNEQPRKISTNLKNKKI", "aa_hexdigest": "f8ed3850db5ed24a03fc2e56871cc953", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 388744, "stop": 389387, "observations": { "deletion": [ 388808 ], "mutation": [ 388916, 389372 ], "stop": [ 388751, 388793, 388799, 388916, 389372 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158BFF58", "query_cov": 1.0, "subject_cov": 0.6558139534883721, "identity": 0.965, "score": 271.0, "evalue": 8.64e-91, "gene_start": 1, "gene_end": 141, "reference_start": 17, "reference_end": 157, "reference_sequence": "MKKNKWKLLTIFGISALPILPLFVTSCNNRETELKNAETIIGNKEIVFNMVDILNSEEESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSEKLNVAFNEQPRKISTNLKNKKIWIYKYMAPVHKLDDKNNKPELRFFYIKQLMLDCSLNKKKTWTWCSFVIYSTPKKRIWF" }, "paralog": false, "identity": 0.9348837209302325, "score": 389.0, "evalue": 7.17e-140, "description": "Internal stop codon at 388751, 388793, 388799, 388916, 389372. Frameshift due to deletion around 388808. Nonsense mutation around 388916, 389372.", "psc": { "uniref90_id": "UniRef90_UPI00158BFF58", "uniref50_id": "UniRef50_D1J8B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158BFF58", "UniRef:UniRef50_D1J8B6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B6" ], "product": "Lipoprotein" } }, "id": "AHCKFKLKGB_688", "locus": "GCA013367975_00676" }, { "type": "cds", "sequence": "contig_1", "start": 389387, "stop": 389626, "strand": "-", "frame": 3, "gene": null, "product": "putative cysteine peptidase", "db_xrefs": [ "RefSeq:WP_175933499.1", "SO:0001217", "UniParc:UPI00158E42F1", "UniRef:UniRef100_UPI00158E42F1", "UniRef:UniRef50_A0A6A8Q0U9", "UniRef:UniRef90_D1J8B7" ], "nt": "ATGCCTAATCCAATAAAAAAAGGAAAAATTGGTCATGCAATCGTTGCTTATGGAATGTATGACAATGGAAATAAACCTCTTTGCCATTATGGATGGGGAGGATATTCTCAAGTAATTGTTTATTCAAGTCTTTCTGGCGAATTATTTTTATTGGGCATGAAACCAAATGGAAAGGTTTATAAAACAAGGAAACATTTTATCAAAAAGGGAATTAAATATTCGGGAAAGGATTTTAAATAA", "aa": "MPNPIKKGKIGHAIVAYGMYDNGNKPLCHYGWGGYSQVIVYSSLSGELFLLGMKPNGKVYKTRKHFIKKGIKYSGKDFK", "aa_hexdigest": "c09e861a04a296c6d1ff611ea2a030cf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158E42F1", "ncbi_nrp_id": "WP_175933499.1", "uniref100_id": "UniRef100_UPI00158E42F1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E42F1", "RefSeq:WP_175933499.1", "UniRef:UniRef100_UPI00158E42F1" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E42F1", "product": "putative cysteine peptidase", "uniref90_id": "UniRef90_D1J8B7" }, "psc": { "uniref90_id": "UniRef90_D1J8B7", "uniref50_id": "UniRef50_A0A6A8Q0U9" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q0U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_689", "locus": "GCA013367975_00677" }, { "type": "cds", "sequence": "contig_1", "start": 390033, "stop": 390212, "strand": "-", "frame": 2, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53", "UniRef:UniRef90_UPI0023AACE53" ], "nt": "ATGCAACAACAAAATAAAAAATATAATTTTGAATTCAAACTTGGTGCAAGCAATAAAATTATTGATTTGGCAGCAAATTTTATTTTGAAATATGGTCAAAAGACATTGCGTGAAGTTGCAAAAGTTTCTTTTAAAACAACAATAAAAGCATTAGAAAAAATAGATAATAAATTAGAATAA", "aa": "MQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLREVAKVSFKTTIKALEKIDNKLE", "aa_hexdigest": "0084eb09148121a8e6683e2b4ad655e1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 390033, "stop": 390341, "observations": { "mutation": [ 390215, 390326 ], "stop": [ 390215, 390326 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AACE53", "query_cov": 1.0, "subject_cov": 0.5784313725490197, "identity": 0.983, "score": 110.0, "evalue": 1.81e-30, "gene_start": 1, "gene_end": 59, "reference_start": 44, "reference_end": 102, "reference_sequence": "MQAISWLDFKKPQNEIIIETKGEEKSLAVAAASIISRKFLIDWMQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLHEVAKVSFKTTIKALEKIDNKLE" }, "paralog": false, "identity": 0.9509803921568627, "score": 182.0, "evalue": 2.94e-61, "description": "Internal stop codon at 390215, 390326. Nonsense mutation around 390215, 390326.", "psc": { "uniref90_id": "UniRef90_UPI0023AACE53", "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AACE53", "UniRef:UniRef50_UPI0023AACE53" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53" ] } }, "id": "AHCKFKLKGB_690", "locus": "GCA013367975_00678" }, { "type": "cds", "sequence": "contig_1", "start": 390324, "stop": 390731, "strand": "-", "frame": 2, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51E35B", "UniRef:UniRef90_UPI001F51E35B" ], "nt": "TTGAATAAAAACTTAGATGAATTAATTGGTGTTGATGAAACGGGAGTAGGAGATTATTTCAGTCCCGTGATTTCAGTTGCCTGTTATATACCAAAAGATAACATTGAAAAACTAAAACAAATTGGTGTTAAAGACAGCAAAAAACTTTCTGATAAAAGAATAATTGAAATTTTTAATCAAATTGAAAATTTAGTTTATTATCAAAAAACTTGCCTTAGTCAAAGTGGTTATAATAAATTAATTGATGCAGGTATTAATAACAACGAAATAAAAACATTAATTCATAGTAATTCAATAAATATATTAACAAAAAAATTAAATCAGAACATTGATGTTTTTATTGATCAATATACAACTTCATTAGAAATGTTGCAAAAGCATTTCAAAAAATTGCAAGCAATTTCTTGA", "aa": "MNKNLDELIGVDETGVGDYFSPVISVACYIPKDNIEKLKQIGVKDSKKLSDKRIIEIFNQIENLVYYQKTCLSQSGYNKLIDAGINNNEIKTLIHSNSINILTKKLNQNIDVFIDQYTTSLEMLQKHFKKLQAIS", "aa_hexdigest": "8ab1c7743e6c47e26e58a7dfe7cf5fda", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI001F51E35B", "query_cov": 0.9259259259259259, "subject_cov": 0.9057971014492754, "identity": 1.0, "score": 246.0, "evalue": 2e-82, "valid": true, "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI001F51E35B" }, "pscc": { "uniref50_id": "UniRef50_UPI001F51E35B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51E35B" ] }, "genes": [], "id": "AHCKFKLKGB_691", "locus": "GCA013367975_00679" }, { "type": "cds", "sequence": "contig_1", "start": 390724, "stop": 391032, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAGATATTACAACATTGACATTGATGATACTGTTGCAATGGGAGATTCATATAATGATTTAAGCATGTATAATGTTGCTAATGTTTGTGTATCACCTGCAAATGCTGAACCCTTAATTAAAAAGATGTCAACAGTTGTTATGAAACAAACAAATAAAGAAGGCGCAGTTGGATACTTTATTGATCAATTCCTAAAAAACCCAGAAAAATACATTAAATTAGGTAAAAAACAAAAGGCCGAACAAAAAAATAATATTAAAACTGTAGGTGCAGATAACTTTTTTAGTAAAGAAGAACAAATTGAATAA", "aa": "MRYYNIDIDDTVAMGDSYNDLSMYNVANVCVSPANAEPLIKKMSTVVMKQTNKEGAVGYFIDQFLKNPEKYIKLGKKQKAEQKNNIKTVGADNFFSKEEQIE", "aa_hexdigest": "c35d3af5988983dafc4f0989cc33846f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 60, "aa_cov": 0.5882352941176471, "hmm_cov": 0.9833333333333333, "evalue": 2.880650508766514e-13, "score": 45.427085876464844, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 11610.111099999991, "isoelectric_point": 6.288575172424316 }, "id": "AHCKFKLKGB_692", "locus": "GCA013367975_00680" }, { "type": "cds", "sequence": "contig_1", "start": 391111, "stop": 391506, "strand": "-", "frame": 1, "gene": "cof", "product": "Putative phosphatase in upp 3'region", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "KEGG:K21064", "SO:0001217", "UniRef:UniRef50_P43051", "UniRef:UniRef90_P43051" ], "nt": "TTGCCTGTGTATGAAAAACTTGGACTTAATGCAATTGTAGGTAATTACAATGGTGCTCACATCCACAACCCTGCTGACCCATTCTTTATACCTGCTATTACATATTTGGACTTAAATGAAGTTCTTTATATTTTAGGCGATGAAAAAGTAAAAAAAGAAATTACTAATTATGCAATAGAAGGTCCTGACTGGGTTCAATTAATGCATAGAGATCCTAACCTTGAAAGAGTGTTTGGATTTAACCAAGCAACAAAATTTAGAGAATGTATTAACCTAGAAAAAATTCCACTAAAACCAACCGGAATTGTATTTGATGTTAAACCTGATACAGATGTTTTGGAATTATTGACATATTTAAAAAGACGTTATGGAGATTTAGGAGAATTTTCTTCTTGA", "aa": "MPVYEKLGLNAIVGNYNGAHIHNPADPFFIPAITYLDLNEVLYILGDEKVKKEITNYAIEGPDWVQLMHRDPNLERVFGFNQATKFRECINLEKIPLKPTGIVFDVKPDTDVLELLTYLKRRYGDLGEFSS", "aa_hexdigest": "c77529f2e3482b857b9e30296c47109d", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [ "cof" ], "truncated": "both", "pseudogene": { "start": 390808, "stop": 391686, "observations": { "mutation": [ 391113, 391518 ], "stop": [ 391113, 391518 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43051", "query_cov": 1.0, "subject_cov": 0.409375, "identity": 0.992, "score": 275.0, "evalue": 2.73e-91, "gene_start": 1, "gene_end": 131, "reference_start": 61, "reference_end": 191, "reference_sequence": "MAYNKESKQRRFLFAIDLDGTLLADSANGTVHPKTEEAIKKAVAQGHIVSIITGRPWRSTLPVYEKLGLNAIVGNYNGAHIHNPADPFFIPAITYLDLNEVLYILGDEKVKKEITNYAIEGPDWVQLMHRDPNLERVFGFNQATKFRECINLEKIPLKPTGIVFDVKPDTDVLELLTYLKRRYGDLGEFSSWSKGEGLSPVFDITSIGIDKGKVISLIMRYYNIDIDDTVAMGDSYNDLSMYNVANVCVSPANAEPLIKKMSTVVMKQTNKEGAVGYFIDQFLKNPEKYIKLGKKQKAEQKNNIKTVGADNFFSKEEEIE" }, "paralog": false, "identity": 0.9931506849315068, "score": 580.0, "evalue": 6.68e-214, "description": "Internal stop codon at 391113, 391518. Nonsense mutation around 391113, 391518.", "psc": { "uniref90_id": "UniRef90_P43051", "gene": "Cof", "product": "Putative phosphatase in upp 3'region", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P43051", "cog_id": "COG0561", "cog_category": "HR", "kegg_orthology_id": "K21064", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43051", "EC:3.1.3.-", "UniRef:UniRef50_P43051", "COG:COG0561", "COG:HR", "KEGG:K21064", "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P43051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43051" ], "product": "Putative phosphatase in upp 3'region" } }, "id": "AHCKFKLKGB_693", "locus": "GCA013367975_00681" }, { "type": "cds", "sequence": "contig_1", "start": 391516, "stop": 391686, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGGCATACAACAAAGAATCAAAACAAAGAAGATTTTTATTTGCTATTGATCTAGATGGAACACTATTAGCAGATTCAGCCAATGGCACAGTTCATCCAAAAACAGAAGAAGCAATTAAAAAAGCTGTTGCCCAAGGACACATTGTTTCAATAATTACAGGAAGACCTTGA", "aa": "MAYNKESKQRRFLFAIDLDGTLLADSANGTVHPKTEEAIKKAVAQGHIVSIITGRP", "aa_hexdigest": "b4070446689c7714536aa9e25714399e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 43, "aa_cov": 0.7678571428571429, "hmm_cov": 0.9534883720930233, "evalue": 6.62788142813121e-12, "score": 40.96463394165039, "start": 14, "stop": 56 } ], "seq_stats": { "molecular_weight": 6108.936900000001, "isoelectric_point": 9.30106334686279 }, "id": "AHCKFKLKGB_694", "locus": "GCA013367975_00682" }, { "type": "cds", "sequence": "contig_1", "start": 391869, "stop": 392498, "strand": "-", "frame": 2, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0009116", "GO:0044206", "RefSeq:WP_020002490.1", "SO:0001217", "UniParc:UPI0003662176", "UniRef:UniRef100_A0A2K9YTA2", "UniRef:UniRef50_Q98QP6", "UniRef:UniRef90_P43049" ], "nt": "ATGGTAAAAATTTTTCAGCATCCCTTAATTAACGCTAAATTAACAACGATGCGTTCAAAAGAAACATCTTACAAAGATTTTAGAGATAATTTGAATGAAATTGCTTCGTTGATGGTATATGAAACATTGAGGGACTATCAAACTAAGAAAATATCAATAACAACACCAATGAATGTTAAATATGAAGGCGAAACTCTTGATAGAGAAATTGTAATCATTCCTATTTTACGTGCGGGGCTTGGAATGTTGAATGGTATTATGGATTTGGTTCCCCAAGCTAGAGTTGGTCATATTGGAATGTATAGAAACGAAGAAACAAACGAAGTTGTTGAATATTTCTTTAAAATTCCAGAAGTACCACATGATAGCTATATTATTATTGTTGACCCTATGCTTGCAACCGGCTCATCTGCATGCGATGCAATAGCAAAATTGGATAAGTTAGGCTTTAGCAATATTAAATTAGTATGTTTAGTAGGCGTTCAACAAGGTATTGATAAAGTAACAAAACAATTTCCTAATGTTGATATATATTTAGCTTCAAAAGATGAAAAATTGAATGAACACAATTATATATTGCCTGGTTTGGGAGATGCTGGCGACCGTATATTTGGAACAAAAATAAAATAA", "aa": "MVKIFQHPLINAKLTTMRSKETSYKDFRDNLNEIASLMVYETLRDYQTKKISITTPMNVKYEGETLDREIVIIPILRAGLGMLNGIMDLVPQARVGHIGMYRNEETNEVVEYFFKIPEVPHDSYIIIVDPMLATGSSACDAIAKLDKLGFSNIKLVCLVGVQQGIDKVTKQFPNVDIYLASKDEKLNEHNYILPGLGDAGDRIFGTKIK", "aa_hexdigest": "3e720a22535f25de2c7be65caa1488af", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003662176", "ncbi_nrp_id": "WP_020002490.1", "uniref100_id": "UniRef100_A0A2K9YTA2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003662176", "RefSeq:WP_020002490.1", "UniRef:UniRef100_A0A2K9YTA2" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTA2", "uniref90_id": "UniRef90_P43049" }, "psc": { "uniref90_id": "UniRef90_P43049", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_Q98QP6", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0009116", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP6" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "AHCKFKLKGB_695", "locus": "GCA013367975_00683" }, { "type": "cds", "sequence": "contig_1", "start": 392526, "stop": 393188, "strand": "-", "frame": 2, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "db_xrefs": [ "COG:COG0274", "COG:F", "EC:4.1.2.4", "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052", "KEGG:K01619", "RefSeq:WP_020002489.1", "SO:0001217", "UniParc:UPI000378BCA6", "UniRef:UniRef100_A0A2K9YTE4", "UniRef:UniRef50_Q98QP7", "UniRef:UniRef90_P43048" ], "nt": "ATGACAGAATTAAACAAATATATTGACCACACAAATTTATCACCTTCTGCAACAAGCAAAGACATTGATAAATTAATTCAAGAAGCAATTAAATATGATTTTAAATCAGTATGTATTGCACCATCATATGTTAAGTATGCTAAAGAAGCATTAAAAAACAGTGATGTGCTTGTTTGCACAGTTATTGGTTTTCCACTAGGATATAATGCAACCAGTGTTAAAGTTTATGAAACAAAAATTGCTGTTGAACATGGTGCTGATGAAATTGATATGGTAATCAATGTTGGAAGATTTAAAGATGGACAATATGAATACGTATTAAACGAAATCAAAGCAATAAAAGAAGCATGCAATGGCAAAACTTTAAAAGTTATAGTTGAAACAGCATTGTTAACAAAAGCTGAATTAATAAAAATTACTGAATTAGTAATGCAATCTGGCGCCGATTTTATTAAAACATCAACCGGATTTTCATATAGAGGAGCTTCATTTGAAGACATTCAAACTATGAAAGAAACTTGCGGTGACAAGCTTTTAATTAAAGCATCAGGTGGTATTAAGAATCTAGCAGATGCACAAGAAATGATTAGATTAGGCGCAAACCGTTTGGGTATGTCTAAATCAGTTCCAGTTATGGAAGAATTATTTAAATTAGAAAAATAA", "aa": "MTELNKYIDHTNLSPSATSKDIDKLIQEAIKYDFKSVCIAPSYVKYAKEALKNSDVLVCTVIGFPLGYNATSVKVYETKIAVEHGADEIDMVINVGRFKDGQYEYVLNEIKAIKEACNGKTLKVIVETALLTKAELIKITELVMQSGADFIKTSTGFSYRGASFEDIQTMKETCGDKLLIKASGGIKNLADAQEMIRLGANRLGMSKSVPVMEELFKLEK", "aa_hexdigest": "58846acd5aac329a1c0a28ee84221e78", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000378BCA6", "ncbi_nrp_id": "WP_020002489.1", "uniref100_id": "UniRef100_A0A2K9YTE4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000378BCA6", "RefSeq:WP_020002489.1", "UniRef:UniRef100_A0A2K9YTE4" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTE4", "uniref90_id": "UniRef90_P43048" }, "psc": { "uniref90_id": "UniRef90_P43048", "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_Q98QP7", "cog_id": "COG0274", "cog_category": "F", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP7" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [ "deoC" ], "id": "AHCKFKLKGB_696", "locus": "GCA013367975_00684" }, { "type": "cds", "sequence": "contig_1", "start": 393215, "stop": 393439, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "ATGAAATTGGGAGCAGGTAGATCTAAAAAAGAAGATTCTATCGACTATGAAGCAGGAATTTATTTGAATAAGAGTTCAAATGAATATGTAAATAAAGGAGATGTATTATTTACATTGTATTCGTCAAAACCTATAAATCCAGAACTTCAAAAAGAATTGTTGTCTGCAATAGAATTTAGCGAAAGCAAGCATGACATTCAAACGGTTTTTGCTAAATTAATGTAA", "aa": "MKLGAGRSKKEDSIDYEAGIYLNKSSNEYVNKGDVLFTLYSSKPINPELQKELLSAIEFSESKHDIQTVFAKLM", "aa_hexdigest": "5fc7271d4064027fce5b036a85fdb1be", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 60, "aa_cov": 0.7972972972972973, "hmm_cov": 1.0, "evalue": 6.579597603849691e-22, "score": 72.3613052368164, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 8359.406099999997, "isoelectric_point": 5.74621753692627 }, "id": "AHCKFKLKGB_697", "locus": "GCA013367975_00685" }, { "type": "cds", "sequence": "contig_1", "start": 393578, "stop": 394189, "strand": "-", "frame": 3, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A386RHI4" ], "nt": "ATGTCAGGAAGAGGACTTGGACATACAGGAGGAACACTTGATAAATTAGAATCAATTGACGGTTATAATTTTTCAGTGACTGACCAACAATTTAGAGACCTAGTTGAAAAACATGGTATTGCAATAGTTGGACAAAATCAAAAACTTGTGCCTGCGGATAAAAAAATCTATGCATTACGTGATGTAACTGGCACCGTTCAATCAATTCCATTGATCGCCTCATCAATTATGTCTAAAAAACTTGCAACTGGTTCAAACTGCATTTTATTGGATGTTAAATGTGGCAATGGCGCATTTATGAAAGATATTAATGAAGCAAAAAAACTTGGAAAATTGATGATTGAAATTGGCAAGAAATTAAATAGAAAGATTGCCGTTGAAATAACCAATATGCAACAACCTTTAGGAAAAACTATTGGCAATAAAATTGAAGTTTTGGAAGCAATAGATACATTAAATGGACACGGACCTAAAGATTTTACAGAAATTATTTACTCATCAGGTTCAACATTATTAGTTTTGGCACAAAAAGCTAAAGATGAAGTTGAAGCAAGAAAAATGATTGATGAAGTAATCAATAATAAAAAAGCATATAACAAATTTTTGGAATGA", "aa": "MSGRGLGHTGGTLDKLESIDGYNFSVTDQQFRDLVEKHGIAIVGQNQKLVPADKKIYALRDVTGTVQSIPLIASSIMSKKLATGSNCILLDVKCGNGAFMKDINEAKKLGKLMIEIGKKLNRKIAVEITNMQQPLGKTIGNKIEVLEAIDTLNGHGPKDFTEIIYSSGSTLLVLAQKAKDEVEARKMIDEVINNKKAYNKFLE", "aa_hexdigest": "c73401cc8d28be398ff1038470d4c93b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A386RHI4", "query_cov": 0.8275862068965517, "subject_cov": 0.8527918781725888, "identity": 0.53, "score": 182.0, "evalue": 3.36e-55, "valid": false, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0A386RHI4", "go_ids": [ "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0A386RHI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A386RHI4" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "AHCKFKLKGB_698", "locus": "GCA013367975_00686" }, { "type": "cds", "sequence": "contig_1", "start": 394301, "stop": 394513, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02885.22" ], "nt": "ATGAGAATTATTGATATTATTAACAAAAAAGTAGAAAATCAAACTCTTACCAAAGAAGAAATTGATTTTTTCATTAAAGAATATGTAGCAGAAAATGTTCCTGACTACCAAGCTGCTGCTTTATTAATGGCAATCAGATTAAATGGTTTAAGCGATGCTGAAACAGCACAATTAACCGACGCTATGATGCATTCAGGCGAAGTACTTGATTGA", "aa": "MRIIDIINKKVENQTLTKEEIDFFIKEYVAENVPDYQAAALLMAIRLNGLSDAETAQLTDAMMHSGEVLD", "aa_hexdigest": "36b15e58d0f699d7be26cfe5892d5407", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02885.22", "name": "Glycos_trans_3N", "length": 62, "aa_cov": 0.8857142857142857, "hmm_cov": 1.0, "evalue": 2.361549973051891e-25, "score": 83.47432708740234, "start": 5, "stop": 66 } ], "seq_stats": { "molecular_weight": 7899.955200000003, "isoelectric_point": 4.414707374572754 }, "id": "AHCKFKLKGB_699", "locus": "GCA013367975_00687" }, { "type": "cds", "sequence": "contig_1", "start": 394530, "stop": 395246, "strand": "-", "frame": 2, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0006139", "KEGG:K03784", "RefSeq:WP_175933500.1", "SO:0001217", "UniParc:UPI00158A3E6D", "UniRef:UniRef100_UPI00158A3E6D", "UniRef:UniRef50_A0A449A549", "UniRef:UniRef90_D1J8C3" ], "nt": "ATGACACCTCATATAAATGCAAAAGATGATGATTTTGCAAAATTAGTTTTAATGCCAGGTGATCCTTTAAGAGCAAAGTATATTGCTGAACACTATTTAGAAAACGCTAAATTAGTGTCAACAGTTAGAAATGTTTTAATGTATACTGGCTTTTATAAGGGAAGAAAAATTTCTATTTGTGCTTCAGGCATGGGCGTTCCTTCAATTGGAATTTATTCATATGAATTATTTAGTCAATATGGAGTTGAAGCAATAATTAGAATTGGATCAGGTGGCTCATTCAAGGCTGATTTAAAAAATTATTCATTAGTTTTGGCAACTTCAGTATATAGTGATAGCACTTCATTCCGTAAAAATTTATTAGTCAATGATGAAAGTAATGTTGCGCTTCCAAATAGCGAATTAAATGATTTAATTAAAAAACATGCTAAGGAATTGAATTTAAAACTTGAAGAAGGAAGAATTTTATCGGAAGATGCCTTTTATTCAGTATTTTCTGTTGAAGATAGAGTTAAAAATTCAGGCGGAGCAGTTGCTGTTGAAATGGAAGCATATGGACTATTTACCGTAGCAGAAAAATTACACAAAAAAGCTGCAACAATTTTAACTATTTCTGATAATTTAATAACACATGAATATACAACCGCTGAAGAAAGACAAAATTCATTCAATCAAATGATGGAATTAGCATTATCAATATCAGAAGATTTTGAATAA", "aa": "MTPHINAKDDDFAKLVLMPGDPLRAKYIAEHYLENAKLVSTVRNVLMYTGFYKGRKISICASGMGVPSIGIYSYELFSQYGVEAIIRIGSGGSFKADLKNYSLVLATSVYSDSTSFRKNLLVNDESNVALPNSELNDLIKKHAKELNLKLEEGRILSEDAFYSVFSVEDRVKNSGGAVAVEMEAYGLFTVAEKLHKKAATILTISDNLITHEYTTAEERQNSFNQMMELALSISEDFE", "aa_hexdigest": "5ca47690f3ca01965d2c5086a11275e0", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158A3E6D", "ncbi_nrp_id": "WP_175933500.1", "uniref100_id": "UniRef100_UPI00158A3E6D", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158A3E6D", "RefSeq:WP_175933500.1", "UniRef:UniRef100_UPI00158A3E6D" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158A3E6D", "uniref90_id": "UniRef90_D1J8C3" }, "psc": { "uniref90_id": "UniRef90_D1J8C3", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_A0A449A549", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0006139" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A549", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A549" ], "product": "Uridine phosphorylase" }, "genes": [ "deoD" ], "id": "AHCKFKLKGB_700", "locus": "GCA013367975_00688" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 395387, "stop": 395677, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 206.2, "evalue": 3.4e-22, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "TTGCATGGCCAACTGCTACTACAAAGTAGAGGAAAATCCGCTCTAGCACTCGCTGTGATGCGAGTAGTGTTCATGCTAGACCCAATAAGTCTAGGCAACAATTTAGTTGACGACTAGTGCAACAGAGACGAGCTTATTTAGTTAAGATGCAACGATGTAAACTCCATGAGCTAGAAACCCAAATTTTGGTAGGGGAACTGGGAACAAGGAACCGAACAAAGAACCCGGAAGCCGATTGGCTTAGATAAATAGTTGGCGCTATTAAAATAGTACAGAAAGCGGTTTATAGTG", "id": "AHCKFKLKGB_41", "locus": "GCA013367975_00689" }, { "type": "cds", "sequence": "contig_1", "start": 395744, "stop": 396298, "strand": "+", "frame": 2, "gene": "recO", "product": "DNA repair protein RecO", "db_xrefs": [ "COG:COG1381", "COG:L", "GO:0003677", "GO:0006281", "GO:0006310", "KEGG:K03584", "SO:0001217", "UniRef:UniRef50_D1J8C4", "UniRef:UniRef90_D1J8C4" ], "nt": "ATGGAATTTGAGCTAAGTGGAATTGTAATTAATAAACAAAATATTAATGAATTTGATGAAATAATATCATTACTAACAGATAAAGGCATTCAAAGAATTTTTTGCCCCGGAGTAAATAAAACTAATTCAAAAAATGGCCCATCAATTTTAATTTTAAACTTAGTTAATTGTGAAGTCATTAAATCAGAAACAAGAAATTTATTGCCTAGACTAAAAACTGCTTCATTAATAAAAAATTTTCCCACAGATTATAGACTTTTAAATATTGCAAATGATTTAAAAACCTTCTTTAGTTGTCTTGATAATTGCAATAATATTAATCTTATTAAAGAATATGGCCATTGCTTAGATTTTATATTGTCAAAATCTGATAAAGTTGAAGCATATTTATTGTTTTTAGTTGTTTCTAGTTTAGGATTTAAGCCCAACTTAAATTCTTGTATTGAATGCAATAATGTTGAAAATATTGTAGATTTTAAACTATACAAAGGAGGATTTTTGTGTTCGATGCATTCAAACATTGTAAATGCTGATGTATCACTATTAAGGTCTTGA", "aa": "MEFELSGIVINKQNINEFDEIISLLTDKGIQRIFCPGVNKTNSKNGPSILILNLVNCEVIKSETRNLLPRLKTASLIKNFPTDYRLLNIANDLKTFFSCLDNCNNINLIKEYGHCLDFILSKSDKVEAYLLFLVVSSLGFKPNLNSCIECNNVENIVDFKLYKGGFLCSMHSNIVNADVSLLRS", "aa_hexdigest": "9e2fd46df687b60ebdd653ef1446e0bc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8C4", "query_cov": 1.0, "subject_cov": 0.8214285714285714, "identity": 0.9840000000000001, "score": 366.0, "evalue": 1.12e-127, "valid": true, "gene": "recO", "product": "DNA repair protein RecO", "uniref50_id": "UniRef50_D1J8C4", "cog_id": "COG1381", "cog_category": "L", "kegg_orthology_id": "K03584", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C4" ], "product": "DNA replication/recombination mediator RecO N-terminal domain-containing protein" }, "genes": [ "recO" ], "id": "AHCKFKLKGB_701", "locus": "GCA013367975_00690" }, { "type": "cds", "sequence": "contig_1", "start": 396402, "stop": 396716, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5", "UniRef:UniRef90_D1J8C5" ], "nt": "GTGGATAAGAATGAACATATTTTTAGTCAATATATTGAAGATAAACAAGACAAGGAAACATTGACTAAAATTTCTGATGATTTTAAAAATCTATATGAATATTTAGAAGATAATTTTAAGCAATACTTACTATTTTATAATGAAGCATTTAATTTAATTATTAATTTACATAAAAAGAATATATCAAAGGAAGATTTAATAAAATTAAATGATTTTGAAGACATGTTATATTCTAATAAAACAAATAATATTGATTTTAAAACATTACAACTTAAGTTTGATGAGATAAAAAAAATATCTATTAACGATAAATAA", "aa": "MDKNEHIFSQYIEDKQDKETLTKISDDFKNLYEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEDMLYSNKTNNIDFKTLQLKFDEIKKISINDK", "aa_hexdigest": "ec79a5dd6a1a5341cdbc92635790ed05", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 396402, "stop": 397016, "observations": { "mutation": [ 396776 ], "stop": [ 396776 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8C5", "query_cov": 1.0, "subject_cov": 0.4928909952606635, "identity": 0.9420000000000001, "score": 187.0, "evalue": 2.44e-58, "gene_start": 1, "gene_end": 104, "reference_start": 108, "reference_end": 211, "reference_sequence": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPNYGIFRNLRDYFEKRVAQYDFKKKIVDKNEHIFSQYIEDKQDKETLAKISDDFKNLFEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEGMLRSNKTNNIDFKTLQRKFDEIKKISINDK" }, "paralog": false, "identity": 0.9656862745098039, "score": 392.0, "evalue": 1.32e-141, "description": "Internal stop codon at 396776. Nonsense mutation around 396776.", "psc": { "uniref90_id": "UniRef90_D1J8C5", "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8C5", "UniRef:UniRef50_D1J8C5" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12700.199899999978, "isoelectric_point": 5.0036729812622065 }, "id": "AHCKFKLKGB_702", "locus": "GCA013367975_00691" }, { "type": "cds", "sequence": "contig_1", "start": 396774, "stop": 397037, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5", "UniRef:UniRef90_D1J8C5" ], "nt": "ATGAAGTTATCAAAAAATCAAGAAAAGGTTCAAAATAAAAAACCACATAGATTTTTAACTAGAAGATCAAAAATAGGCATCATTTCAGGAATTAGTGCAGTTATAGGATTGCCTTTTTTACTAGGTATTACTTATGTTCTTGTTAATAATCATAAAAAAACTAATTCAAACAGTCAATTAATAATAATTCGTAAAGAAATTTATAAGCAATATTTACGTTCATTAAATTTAATTCATTCATTGCCTAATTATGGTATATTTTGA", "aa": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPNYGIF", "aa_hexdigest": "b8fc13f867f179a3a78165c64892718f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 396471, "stop": 397037, "observations": { "mutation": [ 396776 ], "stop": [ 396776 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8C5", "query_cov": 1.0, "subject_cov": 0.41232227488151657, "identity": 1.0, "score": 171.0, "evalue": 2.88e-52, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPNYGIFRNLRDYFEKRVAQYDFKKKIVDKNEHIFSQYIEDKQDKETLAKISDDFKNLFEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEGMLRSNKTNNIDFKTLQRKFDEIKKISINDK" }, "paralog": false, "identity": 0.9680851063829787, "score": 362.0, "evalue": 2.55e-130, "description": "Internal stop codon at 396776. Nonsense mutation around 396776.", "psc": { "uniref90_id": "UniRef90_D1J8C5", "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8C5", "UniRef:UniRef50_D1J8C5" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10047.819399999991, "isoelectric_point": 10.718271827697755 }, "id": "AHCKFKLKGB_703", "locus": "GCA013367975_00692" }, { "type": "cds", "sequence": "contig_1", "start": 397095, "stop": 397274, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTCAATTAAAGCAACTAATAATTGATAGATTAGCCCCATTCTTTTATAAAAATAAACGTAGAGACGTTGTTATTATTCCTACAATTTTGTTTACAAATCATACTACTGCAAGTATTGAAGAAGATCCAAACCTTGATGAAAATGTTATCCTATTGGCAAAATCTACTAAAAAATAA", "aa": "MPQLKQLIIDRLAPFFYKNKRRDVVIIPTILFTNHTTASIEEDPNLDENVILLAKSTKK", "aa_hexdigest": "487e9ca3fe41d15f57e66c0146051a14", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6851.9645, "isoelectric_point": 9.222669792175292 }, "id": "AHCKFKLKGB_704", "locus": "GCA013367975_00693" }, { "type": "cds", "sequence": "contig_1", "start": 397278, "stop": 397688, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17770.6" ], "nt": "ATGCCTTATCACGGCGAATATAGAATGAGCGTTGTTCACGGCTATACTGCTATTGAAAGCGGTGTTGATGCTAAAAACATTTTGATTGCTAGATTAGGGGATGTTTATTATTTAGAAAATCATCAAGTTCGTTTGTCGAATGAAAAGGTTTATTTTGGGCCTGTTTTTATTGATGGTAATATTTTATCCAAAACCAACTCTCAAATTATTAAAGAACGTAGTGAATTAGGCCAAAATGGATTTATGCACGTTGTTTTAGCAATTAATAAAGAAAAGAATCTAATTATTGGTAAACCAAGAATAGTTTCTCGTGGTGCATTTTATGTAAAAAATTCATTGCATTTAATTGAAGAAGCAAAGAGATTGGTTCATGGCGCTATATTATATACAATTAAAAACTCAAATGATTGA", "aa": "MPYHGEYRMSVVHGYTAIESGVDAKNILIARLGDVYYLENHQVRLSNEKVYFGPVFIDGNILSKTNSQIIKERSELGQNGFMHVVLAINKEKNLIIGKPRIVSRGAFYVKNSLHLIEEAKRLVHGAILYTIKNSND", "aa_hexdigest": "20886ae1a00e5afdf394dd569c4a12bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17770.6", "name": "RNase_J_C", "length": 60, "aa_cov": 0.4411764705882353, "hmm_cov": 1.0, "evalue": 6.445925126716824e-14, "score": 48.14616012573242, "start": 69, "stop": 128 } ], "seq_stats": { "molecular_weight": 15400.56679999999, "isoelectric_point": 9.300031852722167 }, "id": "AHCKFKLKGB_705", "locus": "GCA013367975_00694" }, { "type": "cds", "sequence": "contig_1", "start": 397911, "stop": 398372, "strand": "-", "frame": 2, "gene": null, "product": "ribonuclease J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080D5B6", "UniRef:UniRef90_UPI003080D5B6" ], "nt": "ATGATGACAGGTGATTTTAGGTTTGACTATACACCTATTGGAAATTTTACAGATTTTAATAAACTTAAACAAATTGGCGATGAAGGTCTTGATGTTTTATTTTCTGATTCAACAAATGCTATGCGTCCAAATCATTCACCATCAGAGTCAGATATTTTAAAAGATATTAAAAAATATATGTTGGAAGCAGAAAAGAAAATCATTGTAACTGCATTTGCTTCTAATTTAACTAGAATTAAAGCAATTATTGAATTAGGCGCTTCTTTAAATAAAAAAGTTGTTGCCTTTGGCCGTTCTATGGTTGATGGAATAGATATTGGTAGAAGACTAGGCTATATTAATGTTCCAGACGAAGTGTTTATTGACAAAAAATCATTAAGCAAATTTGGAGATAATGAATTATTAATTTTAACAACTGGTTCTCAAGGTGAAGAACTAGCTGGACTTGCAAAGATGTCATGA", "aa": "MMTGDFRFDYTPIGNFTDFNKLKQIGDEGLDVLFSDSTNAMRPNHSPSESDILKDIKKYMLEAEKKIIVTAFASNLTRIKAIIELGASLNKKVVAFGRSMVDGIDIGRRLGYINVPDEVFIDKKSLSKFGDNELLILTTGSQGEELAGLAKMS", "aa_hexdigest": "e42de43bff8d4d1683c236ec3a4bfa47", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 397956, "stop": 398504, "observations": { "mutation": [ 398438, 398468 ], "stop": [ 398438, 398468 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI003080D5B6", "query_cov": 0.9019607843137255, "subject_cov": 0.7540983606557377, "identity": 0.855, "score": 233.0, "evalue": 2.82e-76, "gene_start": 1, "gene_end": 138, "reference_start": 46, "reference_end": 183, "reference_sequence": "TNVEFIEINKDLVVKFGECSDDWWTAQHSIPDAFGVRVKTPTGSLMLTGDIRFDYTPIGNLTDLSKLMKIGEEGLDVLFSDSTNAMRPNHSPSASDILKDIKKYMLEAEKKSIVTAFASKLTRIKAIIELGASLGKKVIAFGRSMIYNIDIGRRLGYINAPDEVFIDKKHLSKYNDNELLILT" }, "paralog": false, "identity": 0.8131868131868132, "score": 295.0, "evalue": 2.94e-103, "description": "Internal stop codon at 398438, 398468. Nonsense mutation around 398438, 398468.", "psc": { "uniref90_id": "UniRef90_UPI003080D5B6", "product": "ribonuclease J", "uniref50_id": "UniRef50_UPI003080D5B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI003080D5B6", "UniRef:UniRef50_UPI003080D5B6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI003080D5B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080D5B6" ], "product": "ribonuclease J" } }, "id": "AHCKFKLKGB_706", "locus": "GCA013367975_00695" }, { "type": "cds", "sequence": "contig_1", "start": 398466, "stop": 398837, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00753.33" ], "nt": "ATGAGCCCAACTAAAATTTTTGGATTAGGCGGAATGCAAGAAATTGGTAAAAGTACTTTGGTTATTCAATATGAAAATCATATTTTTATTATTGACACCGGAATTAAATTTTGCGATTCATATGTAACAGGTGTAAATGGTTCTATTCCAGATTTCACTTTTTTAGCAGAAAATCAAGATAAAATCGAAGGTCTTTTCATAACCCACGGACATGAAGATCATATTGGAGGTGTTCCATATTTAGTTCAACAAGTTGATGTTAAAAAAATATTTGCACCTAGAATTGCTATTCAATATCTAAAACTAAAATTTGAGGATATGAAGATAAAACGTAATATTGAATTTATTGAAACTGAAAAAGATGCAGTTTGA", "aa": "MSPTKIFGLGGMQEIGKSTLVIQYENHIFIIDTGIKFCDSYVTGVNGSIPDFTFLAENQDKIEGLFITHGHEDHIGGVPYLVQQVDVKKIFAPRIAIQYLKLKFEDMKIKRNIEFIETEKDAV", "aa_hexdigest": "5657dcf5c352ec4d827c4c65c5ef9a20", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00753.33", "name": "Lactamase_B", "length": 77, "aa_cov": 0.6260162601626016, "hmm_cov": 0.9090909090909091, "evalue": 4.9480256565819995e-12, "score": 41.480987548828125, "start": 18, "stop": 94 } ], "seq_stats": { "molecular_weight": 13935.941499999986, "isoelectric_point": 5.498797416687012 }, "id": "AHCKFKLKGB_707", "locus": "GCA013367975_00696" }, { "type": "cds", "sequence": "contig_1", "start": 399250, "stop": 399447, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGTAATAGCAAAAGCTAATTGGTCATTACACAAAGTTCCAATAATACTTATTATTGTTACATTATGTTTGCCAATTCTATTATTATGTGAAATAGTTAGAATAGTATCACTATTTTTAATAGTAATAAAAAAGCAAAAAAATTTTAATAGCCAAGTATATTATGGACCACAAGTTTCTAACATACAAAATTAA", "aa": "MLVIAKANWSLHKVPIILIIVTLCLPILLLCEIVRIVSLFLIVIKKQKNFNSQVYYGPQVSNIQN", "aa_hexdigest": "e730064c48e213a4a784a910f5f0e9b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7418.054700000001, "isoelectric_point": 9.698317527770996 }, "id": "AHCKFKLKGB_708", "locus": "GCA013367975_00697" }, { "type": "cds", "sequence": "contig_1", "start": 399513, "stop": 400448, "strand": "-", "frame": 2, "gene": "trxB", "product": "Thioredoxin reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "RefSeq:WP_020002482.1", "SO:0001217", "UniParc:UPI00037C1753", "UniRef:UniRef100_A0A6A8PY71", "UniRef:UniRef50_A0A448ZW45", "UniRef:UniRef90_D1J8C8" ], "nt": "ATGGAAAATAATAACTTATATGATGTTTTAATAATTGGGGCAGGGCCAGCAGGATTAACGGCTGCAATATATTTAGCAAGAAATGACATAAATGTTGCTTTTATTGAAGGCGGAGTACCAGGAGGAAAAGTTTCTGCTCAATCTAAAATTGAAAATTATCCAGGTTTTAGTTTTATAAGTGGTTTTGAACTTTCTAAAGCCATGCTTAAACAAGCAAGAGATAATGGCTCAAAATTTATTTATGGTAATGTAAAGGAAATTAATTCTATATCAGATATTGAAAAAGAAGTCATTTTGGAAAATGGCATGTCAATAAAATCAAAAGCAATAATTATTGCAACTGGCATGAAAGAAATTGTTCCTTTGGATGTAAAAGGTATTGAAGAATATAGAGGCAAAGGTGTATCATATTGCGTAATTTGCGATGGTCCTTTATATAAAAATAAACCCTGTGCAATCATTGGCGGTGGTAATTCAGCATTCGAAGAATCATCTTATTTAGCATCAATTGCCTCTGAGGTTCATATTTTTGTTCGTGATGGAATTATTGCAGAAAAAAAACTTGTCAATGAAGCAATGTCTAAAAGTAATATTTATATTCATGAAAATTCAACTATTAAAGAAATTTTGGGCAATGAAAAAGTTGAAGAAATTATTGCTAATGTAAATGGAAAAGAAACAAAAATGAATATAAGTTGCATTTTTCCTTATGTTGGTTTTAAACCTAACACAGCATTTATAAAAGGTAAATACGATGAAATATTAAATAGCCGTGGATTTATTGAAGTTAATGAAAATATGGAAACTAAAATTCCTAATATTTTTGCAATAGGCGATGTTATTGTAAAAGATGTAAGACAAATTACAACAGCAACAGCCGACGGGACAATTGCTGCAAGAGCAATAAGTAATAAAATCGATAAATTATATAAATAA", "aa": "MENNNLYDVLIIGAGPAGLTAAIYLARNDINVAFIEGGVPGGKVSAQSKIENYPGFSFISGFELSKAMLKQARDNGSKFIYGNVKEINSISDIEKEVILENGMSIKSKAIIIATGMKEIVPLDVKGIEEYRGKGVSYCVICDGPLYKNKPCAIIGGGNSAFEESSYLASIASEVHIFVRDGIIAEKKLVNEAMSKSNIYIHENSTIKEILGNEKVEEIIANVNGKETKMNISCIFPYVGFKPNTAFIKGKYDEILNSRGFIEVNENMETKIPNIFAIGDVIVKDVRQITTATADGTIAARAISNKIDKLYK", "aa_hexdigest": "363540d965586c386bd51304437db1bf", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00037C1753", "ncbi_nrp_id": "WP_020002482.1", "uniref100_id": "UniRef100_A0A6A8PY71", "db_xrefs": [ "SO:0001217", "UniParc:UPI00037C1753", "RefSeq:WP_020002482.1", "UniRef:UniRef100_A0A6A8PY71" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PY71", "uniref90_id": "UniRef90_D1J8C8" }, "psc": { "uniref90_id": "UniRef90_D1J8C8", "gene": "TrxB", "product": "Thioredoxin reductase", "uniref50_id": "UniRef50_A0A448ZW45", "cog_id": "COG0492", "cog_category": "O" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZW45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZW45" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "AHCKFKLKGB_709", "locus": "GCA013367975_00698" }, { "type": "cds", "sequence": "contig_1", "start": 400482, "stop": 400739, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACCTTTACGTGATGGCAGTTATACTATTTATAAAGTAATTTCTGCTTTATATATAATAGTTGGTTTGGTATTGACTGTAATGTTTGAATTCGTTATAAAATTGAATTACAATGTTTATAAAGTTAATACATATAATAACGAAAAGCTTTCAAAATTCTTTTACTTTATAGTTTATGAACCTAAAAATTCAAACTCATTTGCATTTAAAAAATCGACTACTAAAAATCAAATAGAAACTAAAACAGCAGCTTAA", "aa": "MEPLRDGSYTIYKVISALYIIVGLVLTVMFEFVIKLNYNVYKVNTYNNEKLSKFFYFIVYEPKNSNSFAFKKSTTKNQIETKTAA", "aa_hexdigest": "8a0fbf5055710196ae29c000861a2e52", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9942.458099999989, "isoelectric_point": 9.456045341491702 }, "id": "AHCKFKLKGB_710", "locus": "GCA013367975_00699" }, { "type": "cds", "sequence": "contig_1", "start": 400821, "stop": 400958, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAAGGTTCTGTCGCATTTGAAATATTGCCAAAATTAATTAGAGATCATATGTTTATTGATGGAAAATACCGTTATCCTTTATTCTTCTATGAATCAATTGCAAACTTAGCTGCTTATATTATCTTAGTTTGA", "aa": "MEKGSVAFEILPKLIRDHMFIDGKYRYPLFFYESIANLAAYIILV", "aa_hexdigest": "73895c3ec48eb0fb9b843951e168c0dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5297.237099999999, "isoelectric_point": 6.526503181457519 }, "id": "AHCKFKLKGB_711", "locus": "GCA013367975_00700" }, { "type": "cds", "sequence": "contig_1", "start": 401502, "stop": 403244, "strand": "-", "frame": 2, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "nt": "GTGTTATTATCCGAAAGTGGCACAAAACGCGAGAAGTTTCAAGTTATTGAAGGCATTGTTGATAAATTACAAAGGAAGTATTTTGATACAAAATCACTAGAAGCTAGGGCTTATTATAGTAAATACATTGGAGATATTACTTGTCCCTCATGCCATGGAAAGCGTTTGAATAAGTATGCATTGTCTTGCAAAATTCTAGATCTAGATATATACGAAATTTGCAATAAGTCGCTTGGAGATTTAGCAATTATTTTAGAAAGATTGCAGTTAAGTGATTTAGAAAAAGAAATTACCTCTTTAATTTTGTCTGAATTAAAACATAGAATTAAATTTTTAATTGATGTTGGCTTAAAGTATTTAACATTGAATAGAAAAGCTGAGACATTAAGCGGTGGTGAAGCTCAAAGAATTAGATTAGCAACACAAATTGGTTCAAATTTAACAGGTATTTTGTATGTTTTAGATGAACCATCAATTGGCTTACATCAAAAAGATAATGAAAAATTGCTAAGCTCTTTAAGAAAAATGGTTGAATTAGGCAATAGTTTAATTGTTGTTGAACATGATGAAGAAACAATTAGACAAGCAGATTATGTAGTTGATATTGGTGTTCAAGCAGGCGAACATGGCGGAAATATTGTTGCATGTGGAAATGTTGAAAATATAATTGCATGTAAGCAAAGCCTAACTGGCCAATATTTGTCGAAAGAAAGACAAATAGAGGTGCCAAAATTTAGACGTAGTGGAAATGGCAAGACATTAACATTAAAAAATGCAAAATTAAATAATTTAAAAAATATTAAAGTAACTTTTCCATTAGGAAAATTTATTGCAATTACTGGAGTTAGTGGTTCTGGAAAATCTACATTAATAAACGATGAACTAGTTGGAAGTCTTAATAAATATTTATTAGATCAATATTATGATATTCAAAGAGATAGTAAATTAATTGGCCAAACTAACATTGATAAATTAATTCAAATTGACCAAAATCCAATCGGACGTACTCCTAGATCAAATCCCGCAACATATACTGGTGTTTTTGATGATATTAGAGAAATTTTTGCAAATACCGAAGAAAGTAAAATTAGAGGATATACAAAATCAAGATTTTCATTTAATGTTTCAGGAGGACGTTGTGATAAATGCGAAGGCGATGGTGTAATCAAAATTGCCATGCATTTTTTGCCTGATGTTTATGTGACATGTGACCATTGCAATGGAAAACGTTATAATCAAGAAACACTAGAAATTAAATATCACGGTAAAAGTATTGGCGATGTTTTGGATATGACAATTGAAAAAGCCTATGCATTATTTATAAACAAGAGCCAAATTAAAGAAAAATTGCAAGTTTTATTAGATGTTGGACTTGGCTATATTAAATTAGGCCAAAATGCTACGACATTAAGTGGTGGTGAAGCTCAAAGGGTAAAATTAGCAACATACCTACAAAAAAAGCCAACAGGCAAATCTTTATATGTATTAGATGAACCAACAACCGGATTACATCCTTACGATATCTCTAATTTATTAATAGTATTAAATAGAATAGTGGACAATGGCGATACAGTAATTGTAATTGAACATAATTTGGATTTAATTAAATGTGCTGATCATATTATTGATTTAGGCCCTGATGGTGGGGACGAAGGGGGAAAAGTTATTTGCTCAGGAACACCTGAACAGGTCTCAAAATGCGAAAATTCGTACACGGGGCAATATTTAGCAAAACTTTTATAG", "aa": "MLLSESGTKREKFQVIEGIVDKLQRKYFDTKSLEARAYYSKYIGDITCPSCHGKRLNKYALSCKILDLDIYEICNKSLGDLAIILERLQLSDLEKEITSLILSELKHRIKFLIDVGLKYLTLNRKAETLSGGEAQRIRLATQIGSNLTGILYVLDEPSIGLHQKDNEKLLSSLRKMVELGNSLIVVEHDEETIRQADYVVDIGVQAGEHGGNIVACGNVENIIACKQSLTGQYLSKERQIEVPKFRRSGNGKTLTLKNAKLNNLKNIKVTFPLGKFIAITGVSGSGKSTLINDELVGSLNKYLLDQYYDIQRDSKLIGQTNIDKLIQIDQNPIGRTPRSNPATYTGVFDDIREIFANTEESKIRGYTKSRFSFNVSGGRCDKCEGDGVIKIAMHFLPDVYVTCDHCNGKRYNQETLEIKYHGKSIGDVLDMTIEKAYALFINKSQIKEKLQVLLDVGLGYIKLGQNATTLSGGEAQRVKLATYLQKKPTGKSLYVLDEPTTGLHPYDISNLLIVLNRIVDNGDTVIVIEHNLDLIKCADHIIDLGPDGGDEGGKVICSGTPEQVSKCENSYTGQYLAKLL", "aa_hexdigest": "5fab0d5a666062e68adebb9ca8779735", "start_type": "GTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A3B0P0N4", "query_cov": 0.9982758620689656, "subject_cov": 0.9538714991762768, "identity": 0.737, "score": 862.0, "evalue": 1.39e-310, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0P0N4", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "product": "UvrABC system protein A" }, "genes": [], "id": "AHCKFKLKGB_712", "locus": "GCA013367975_00701" }, { "type": "cds", "sequence": "contig_1", "start": 403362, "stop": 403718, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGATAGAGTTAAATTATCAAATGATGATGATATAAAATCAAGAATATCAAGTGCAATTGAGGTTGCATTAGAGCAATCTAATGGTTTAATTTCAACTATTGTTAATGAAAGTAAAAAGAATACTTATTCAATACATCATAGTTGCAGTTTTGGCGACTTTGATATGCCAAAAATTGAACCTAAATTATTCTCGTTTAATTCTCCAGCTGGAATGTGCCCTGAATGCAAGGGGCTTGGTGTTATTCAAAAGGCAAGCTTTGATTTAATCTGTCCCGATAGGCAGCTTTCTATAAACGAAGGGGCAATAAAGTATTATAGAAATTTCATGAATACATACAATTTAGAATGA", "aa": "MFIDRVKLSNDDDIKSRISSAIEVALEQSNGLISTIVNESKKNTYSIHHSCSFGDFDMPKIEPKLFSFNSPAGMCPECKGLGVIQKASFDLICPDRQLSINEGAIKYYRNFMNTYNLE", "aa_hexdigest": "fbbc537df1fec16720c87644cd9139fb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13292.003799999984, "isoelectric_point": 5.665392494201661 }, "id": "AHCKFKLKGB_713", "locus": "GCA013367975_00702" }, { "type": "cds", "sequence": "contig_1", "start": 403728, "stop": 404330, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17760.6" ], "nt": "ATGAACGATAAAATAATAATAAAAGGTGCTAGAGAAAATAATTTAAAAAATATTAATTTAGATATTCCTCGTAACAAATTTGTGGTTTTTACTGGGGTTAGTGGCTCGGGTAAAAGTTCTTTAGCATTTAATACAATCTATGAAGAAGGCAGACGTAGGTATGTTGATAGCCTTTCTAATTATGCAAAACAATTTTTAGGTGGTACTAAAAAACCTAATGTTGATAGCATTGAAGGATTGAGCCCAGCAATTTCTATTGAACAAAAAACAGTACATGTTAATCCCAGAAGTATAGTTGGTACTGTTACTGAAATTTATGATTATTTAAGATTGCTATATGCAAGAGTAGCAAAACCTTATTGTCCAAATCACAAAATTGAAATTACTGCTCAAAAAACAAAAGACATTATTTCTAACATTTATAAAAACCCATTAGGATCAACAATATACATATTAGCCCCATTAGTAAATAATCAAAAAGGAAGCCACCAAGTATTACTTAATAAACTAAAAAGAGATGGTTTTATAAAAGTCTTGATAAATGACGAGATTTATTTCTTAGAAAATGTTGATTCAATTAATCTAGATAAAAATAAACGTTGA", "aa": "MNDKIIIKGARENNLKNINLDIPRNKFVVFTGVSGSGKSSLAFNTIYEEGRRRYVDSLSNYAKQFLGGTKKPNVDSIEGLSPAISIEQKTVHVNPRSIVGTVTEIYDYLRLLYARVAKPYCPNHKIEITAQKTKDIISNIYKNPLGSTIYILAPLVNNQKGSHQVLLNKLKRDGFIKVLINDEIYFLENVDSINLDKNKR", "aa_hexdigest": "d68f6e1ecdefd5eaf0bea1d643073187", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_W7DIQ3", "query_cov": 0.99, "subject_cov": 0.9611650485436893, "identity": 0.5660000000000001, "score": 231.0, "evalue": 4.38e-74, "valid": false, "product": "UvrABC system protein A", "uniref50_id": "UniRef50_W7DIQ3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17760.6", "name": "UvrA_inter", "length": 69, "aa_cov": 0.345, "hmm_cov": 0.9710144927536232, "evalue": 1.6210600126462713e-19, "score": 65.13473510742188, "start": 131, "stop": 199 } ], "seq_stats": { "molecular_weight": 22612.763600000024, "isoelectric_point": 9.641972160339353 }, "id": "AHCKFKLKGB_714", "locus": "GCA013367975_00703" }, { "type": "cds", "sequence": "contig_1", "start": 404476, "stop": 405033, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2DDAE5", "UniRef:UniRef90_UPI001E2DDAE5", "PFAM:PF07804.18" ], "nt": "GTGGATGGCGAAATTCATTTATATAAACGAAACTCAAAATTTGCAAATAAAAACTTATACGATGTTTATAGTGAACATTTTGCAAGTCAAGTTGCCAAATTTTTGAATTTACCGGCAGTTGAATATAAAATTACTAAGTATGGAAAGCATTTTGTTAGTGATTGCAAATTGTTTACAAACAAAGATTTTTCATTTATCCCATTTTCTAAAATCATAGATCCCAATAGTATTGATAATAATTTAAAACTATGTAGTCAGATTGCTTTTTATTATGGCAAAGAGTTATTTGAAGATTTAATGGTTTTTGATGCTTTAATTTGCAATGGGGATAGACATTTAAATAATTTTGGCATGATGTTGGACAATAATAGCGGAATTTTAACAAAGCCAGCTCCAATATTTGATTTCAACAATAGTTTAATATTTGATTATCCTATTTATTTAAAATCAATTGCAAAAACTGCATTTTTAAATGACTATGAGAATCGCAAGACTAGGTTTTTTGAAAGTTTTAATCAACAACTAAAAATTTTTATTAGAAAAAGACATTTAGATTGA", "aa": "MDGEIHLYKRNSKFANKNLYDVYSEHFASQVAKFLNLPAVEYKITKYGKHFVSDCKLFTNKDFSFIPFSKIIDPNSIDNNLKLCSQIAFYYGKELFEDLMVFDALICNGDRHLNNFGMMLDNNSGILTKPAPIFDFNNSLIFDYPIYLKSIAKTAFLNDYENRKTRFFESFNQQLKIFIRKRHLD", "aa_hexdigest": "492b10126e7669c18423629de9b42c20", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 404320, "stop": 405234, "observations": { "mutation": [ 404478, 405042, 405072, 405183, 405219 ], "stop": [ 404478, 405042, 405072, 405183, 405219 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2DDAE5", "query_cov": 1.0, "subject_cov": 0.6085526315789473, "identity": 0.978, "score": 373.0, "evalue": 4.98e-129, "gene_start": 1, "gene_end": 185, "reference_start": 68, "reference_end": 252, "reference_sequence": "MCNSFWICSQEESCKLNWEDVNYFDHFNNDSCNYFLDYKAKLTETNRLQIKSPSWFIDGDMLKAWVEVDGEIHLYKRNSKFANKNLYDVYSEYFASQVAKFLNLSAVDYKITKYGKHFVSDCKLFTNKDFSFIPFSKIIDPNSIDNNLKLCSQIAFYYGKELFEDLMVFDALICNGDRHLNNFGMMLDNNSGILTKPAPIFDFNNSLIFDYPIYLKSIAKTAFLNDYENRKTRFFESFNQQLKIFIRKRHLDWIKKLKKFKFKQPNKIKMDMKYLKFINKFFKKRIKKAEQYLIEKGYIKENER" }, "paralog": false, "identity": 0.9638157894736842, "score": 597.0, "evalue": 6.19e-220, "description": "Internal stop codon at 404478, 405042, 405072, 405183, 405219. Nonsense mutation around 404478, 405042, 405072, 405183, 405219.", "psc": { "uniref90_id": "UniRef90_UPI001E2DDAE5", "uniref50_id": "UniRef50_UPI001E2DDAE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2DDAE5", "UniRef:UniRef50_UPI001E2DDAE5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2DDAE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2DDAE5" ] } }, "hypothetical": true, "pfams": [ { "id": "PF07804.18", "name": "HipA_C", "length": 104, "aa_cov": 0.5459459459459459, "hmm_cov": 0.9038461538461539, "evalue": 9.420494268806864e-10, "score": 33.78028869628906, "start": 76, "stop": 176 } ], "seq_stats": { "molecular_weight": 21783.736300000008, "isoelectric_point": 8.627497673034668 }, "id": "AHCKFKLKGB_715", "locus": "GCA013367975_00704" }, { "type": "cds", "sequence": "contig_1", "start": 405349, "stop": 405495, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACATATTATTTAAAATGTAAAAACAAGATTTGTTTAAGTTTTGAAGCAGATGAAAAATTAACAACATTGAGCAATGTTAAATTGCTTGATGATTTTATTTTTATAAAGCAAAAATACAATGAATATTCGCCAAGACAATGA", "aa": "MKTYYLKCKNKICLSFEADEKLTTLSNVKLLDDFIFIKQKYNEYSPRQ", "aa_hexdigest": "366f812ebc9389176d9af3cf518abcec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5812.731900000001, "isoelectric_point": 8.97594928741455 }, "id": "AHCKFKLKGB_716", "locus": "GCA013367975_00705" }, { "type": "cds", "sequence": "contig_1", "start": 405502, "stop": 405660, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BCA40", "UniRef:UniRef100_Q8RK97", "UniRef:UniRef50_A0A454CA89", "UniRef:UniRef90_A0A454CA89" ], "nt": "ATGCTAGAAAATTTACAAAAAATATTGCACCGTGATCTTTCAAAAGAAATTGCAGAAGTTGAAGAAAAGTATAAAAATCAAGCAAAGGAAGACCACAAAGAAGATCCAAAAACAAAATCAAAAGATAATAAGAACGATTTTGATGGCGAAATAAATTAG", "aa": "MLENLQKILHRDLSKEIAEVEEKYKNQAKEDHKEDPKTKSKDNKNDFDGEIN", "aa_hexdigest": "9bddfb4c4a295b23be16d0370265a4b8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000BCA40", "uniref100_id": "UniRef100_Q8RK97", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BCA40", "UniRef:UniRef100_Q8RK97" ] }, "ips": { "uniref100_id": "UniRef100_Q8RK97", "uniref90_id": "UniRef90_A0A454CA89" }, "psc": { "uniref90_id": "UniRef90_A0A454CA89", "uniref50_id": "UniRef50_A0A454CA89" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA89" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6184.768400000002, "isoelectric_point": 5.288891029357909 }, "id": "AHCKFKLKGB_717", "locus": "GCA013367975_00706" }, { "type": "cds", "sequence": "contig_1", "start": 405670, "stop": 406092, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2C38EE", "UniRef:UniRef90_UPI001F2C38EE" ], "nt": "ATGTCAATCGAAGATTATGATGGCGGGTTTGGAACTAAGGAATTTTCTCTTGAATCTGAATTATCAGAAGACGAAGAATCTGATGAAAAATCAGATGATGGCGATTCAAATGATGAAGAAATAGATATGAGCAGTATGGATGATTTTAAAAATTCATTTGTAAATTTCTTAGAAGGTTTTGATGATTTAAAGCAACAAGCAAAAAAATTAAAAGAACAAATTGCTGATAATAAATTTATGTCTGGTTCTCCATTTGAAATGAATTATGAAGATCTAAGTATGGAAAAATTGGTTGATAGTATGTATAAAGCTGCCAAAGCAATTGGAAATGTTCGTGATGACGAAATGGTTGATTATTTCAAGGGCGCATTCGATGAACTAAGGGGAATGGGAATAAATAATATTCCTAATGATGGTTCATGA", "aa": "MSIEDYDGGFGTKEFSLESELSEDEESDEKSDDGDSNDEEIDMSSMDDFKNSFVNFLEGFDDLKQQAKKLKEQIADNKFMSGSPFEMNYEDLSMEKLVDSMYKAAKAIGNVRDDEMVDYFKGAFDELRGMGINNIPNDGS", "aa_hexdigest": "ff5801b1df743f872f7cfd637333fe06", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 405502, "stop": 406165, "observations": { "insertion": [ 406108 ], "stop": [ 405672 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2C38EE", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 0.993, "score": 263.0, "evalue": 1.09e-87, "gene_start": 1, "gene_end": 140, "reference_start": 25, "reference_end": 164, "reference_sequence": "MKFSREFIGKQNLSNRRSIPIERRMSIEDYDGGFGTKEFSLESELSEDEESDEKSDDGDSNEEEIDMSSMDDFKNSFVNFLEGFDDLKQQAKKLKEQIADNKFMSGSPFEMNYEDLSMEKLVDSMYKAAKAIGNVRDDEMVDYFKGAFDELRGMGINNIPNDGSWEKSMLENLQKILHRDLSKEIAEVEEKYKNQAKEDHKEDPKTKSKDNKNDFDGEIN" }, "paralog": false, "identity": 0.9727272727272728, "score": 397.0, "evalue": 7.49e-143, "description": "Internal stop codon at 405672. Frameshift due to insertion around 406108.", "psc": { "uniref90_id": "UniRef90_UPI001F2C38EE", "uniref50_id": "UniRef50_UPI001F2C38EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2C38EE", "UniRef:UniRef50_UPI001F2C38EE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2C38EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2C38EE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15832.989399999991, "isoelectric_point": 4.0500284194946286 }, "id": "AHCKFKLKGB_718", "locus": "GCA013367975_00707" }, { "type": "cds", "sequence": "contig_1", "start": 406264, "stop": 406635, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2E838F", "UniRef:UniRef90_UPI001F2E838F" ], "nt": "TTGTCAGATTTAAACACTTTTAATTCCCTAAATATTTTTAATGATTTTTTAATAAACGATGATGAAGAAAATAAAAATTCTTTTGACTTTAAAAAATATGGACTTGAACAAAATGAAATTATTTTCTCTGCAATTTTGCCTACAAAAGAAATTATTTCATTGGCATTAAAAATTGATTTTGTTTATGACGATAAAAGAGAATTTGATGAAGATTTTCAAATTGATCCTGAATTAATTAGTCAAAGTGTAATTTCCGAAAGAAAAGAAACTTTAAAATATTTAAAAGAATTAAAGCAAAATGAATTTAAAAATTTTCACACAAACCCTTCAAATGATATTAAAAATATAACTAGAAGTGATTTTAGCAAATGA", "aa": "MSDLNTFNSLNIFNDFLINDDEENKNSFDFKKYGLEQNEIIFSAILPTKEIISLALKIDFVYDDKREFDEDFQIDPELISQSVISERKETLKYLKELKQNEFKNFHTNPSNDIKNITRSDFSK", "aa_hexdigest": "05ddce20e792826eca5ce3b83712c8fe", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 406295, "stop": 406923, "observations": { "insertion": [ 406328, 406341 ], "mutation": [ 406716 ], "stop": [ 406303, 406716 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2E838F", "query_cov": 0.8048780487804879, "subject_cov": 0.47596153846153844, "identity": 0.97, "score": 183.0, "evalue": 1.12e-56, "gene_start": 1, "gene_end": 99, "reference_start": 97, "reference_end": 195, "reference_sequence": "MIKKQLGINLKLTVRELNVAFDYEFKPIEAVKKINFEPISFPITSGISFSELVDVLGAFFGFEKSVVFHWYTIPNYLKTIVPFEFVFGDLQASQEFLSDLNTFNSLNIFNDFLINEDEENKNSFDFKKYGLEQNEIIFSAILPTKEIISLALKIDFVYDDKREFDEDFQIDPELISQSVISERKETLKYLKELKKMNLKFSYKSFKWY" }, "paralog": false, "identity": 0.9711538461538461, "score": 387.0, "evalue": 2.32e-139, "description": "Internal stop codon at 406303, 406716. Frameshift due to insertion around 406328, 406341. Nonsense mutation around 406716.", "psc": { "uniref90_id": "UniRef90_UPI001F2E838F", "uniref50_id": "UniRef50_UPI001F2E838F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2E838F", "UniRef:UniRef50_UPI001F2E838F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2E838F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2E838F" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14586.074499999979, "isoelectric_point": 4.589316749572753 }, "id": "AHCKFKLKGB_719", "locus": "GCA013367975_00708" }, { "type": "cds", "sequence": "contig_1", "start": 406714, "stop": 406923, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAGAAACAATTAGGAATAAATCTAAAATTAACAGTTAGAGAATTAAATGTAGCTTTTGACTACGAATTTAAACCAATAGAAGCAGTTAAAAAAATTAATTTTGAACCAATATCATTTCCAATAACTTCAGGGGTTTCTTTTTCTGAATTAGTGGATGTTTTAGGCGCATTTTTTGGCTTTGAAAAGAGTGTTGTATTTCATTGA", "aa": "MIKKQLGINLKLTVRELNVAFDYEFKPIEAVKKINFEPISFPITSGVSFSELVDVLGAFFGFEKSVVFH", "aa_hexdigest": "1b31cfaa840b87f55f8e97b1cb728602", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7824.093599999998, "isoelectric_point": 6.552023887634278 }, "id": "AHCKFKLKGB_720", "locus": "GCA013367975_00709" }, { "type": "cds", "sequence": "contig_1", "start": 406916, "stop": 407197, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTTAGAAAATGTTTATAAGAATGTAGAAACATCTAAAGATGAAATAATGACAATCGGGGATGGAAATAATGATATACCAATGTTGTCAATATCAAAATATTCATATGCAATGGCAAATGCAACACAAACTGTACATGAACATAGCCACTTTCATACAAGTGCTGTTGAACAAAATGGTTTGGGAGAAGCAATACTTGATTACATATATAGATTAAAAAATATAACCAAAAAATATATGTTGCATGAATTTGAAAACAATAGAGGAGCAGAAAATGATTAA", "aa": "MLENVYKNVETSKDEIMTIGDGNNDIPMLSISKYSYAMANATQTVHEHSHFHTSAVEQNGLGEAILDYIYRLKNITKKYMLHEFENNRGAEND", "aa_hexdigest": "f986a217e3e43ad8678e3b360fbf529f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 64, "aa_cov": 0.6881720430107527, "hmm_cov": 1.0, "evalue": 1.3231767314441105e-18, "score": 62.91756057739258, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 10654.730299999992, "isoelectric_point": 5.228414344787596 }, "id": "AHCKFKLKGB_721", "locus": "GCA013367975_00710" }, { "type": "cds", "sequence": "contig_1", "start": 407561, "stop": 407836, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAGTCAAACAATTTTAAAAGTTACTAACAACAAGAAGCCAATTGCTTTTTCAGATGTTGATGGTACTATATATAAAAATTTTAATTTAAAGCAAGAAACTATTGATGATATCCATTTTGCAGTAGACAATGGATTAGATTTTAATATATGCACCGGAAATCCAATTCAAGAAAGAATGTTGAAACTAGCCGAAACATTAAAAGCGAGATATTTAATAGGATCTTCCGGCGCTCAAATTTTTGATATGCAATCAAGAGAATATATTGCCTGTTGA", "aa": "MSQTILKVTNNKKPIAFSDVDGTIYKNFNLKQETIDDIHFAVDNGLDFNICTGNPIQERMLKLAETLKARYLIGSSGAQIFDMQSREYIAC", "aa_hexdigest": "3d09afb544d3af4ccad0b0724e2ecdca", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 75, "aa_cov": 0.8021978021978022, "hmm_cov": 0.9733333333333334, "evalue": 3.1985094522604097e-10, "score": 35.44811248779297, "start": 17, "stop": 89 } ], "seq_stats": { "molecular_weight": 10276.648599999993, "isoelectric_point": 5.686195564270019 }, "id": "AHCKFKLKGB_722", "locus": "GCA013367975_00711" }, { "type": "cds", "sequence": "contig_1", "start": 407853, "stop": 408656, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175933510.1", "SO:0001217", "UniParc:UPI00158B1EB6", "UniRef:UniRef100_UPI00158B1EB6", "UniRef:UniRef50_A0A454CA04", "UniRef:UniRef90_A0A454CA04" ], "nt": "ATGAATAAAAAACAAATAAAAAAATTCTTGGGTTTATCATTGACTATTCCAACTTTACTAGCTATCCCATTAGTTTCATTATCATGCAAAAAATCAAAAGATAATCAAGATAATTTAGAATTAGGCAATGATGATGGAATTGAAAAACAAGATTTAAGTGTTGCTAAAAGAATAGTTGAAATATTTAAAGAACACAGATTTTCATTTGGTGAAATGAATCAAAAATTATTCTTACAAAATCAAAATATAGAAGACCCTAAAAAACCATTTAAAAAAATAAGATTTGAAGATTTTTATAACAATAGAGTAAAAGATTGGAATTTTAAAGAAAAGTCTGAATACAATAGAGCTTTGGAGTTGGATAATGATCTTAAATATCCCTTCTTTGATATAAAGAATCAAAATAGTTTTATTAATTTATTAAAAAATGATCCTGATCCTAATCTTTATAGGGTTTTTAATAAATATTTTCAAATAAGATTAAGAACAAATGATGAACTTTTAAAAATACTTTTTGTGTTTGAATTAGTATCAAAAAAAATGCAACAACAATTAAGTAATGAAATAAAAAAGGACGCATCGGATTTAAATGTAAAGAATTATTTCAATTTAAAAACATATGCCATAAATCATTTTTTATTAGATTATACGGAATTTTTAGAATCAGAAGAACCCGAAACTACAATGCATTATACATTTAGATTATTAAGTTATATTGTGCCACCAGTTGCCATTTCTAGCATTATAATTTATATTATTATAATGGTATTAAAAAAGAGGCATAAATCAAAGACAAGAGCATAA", "aa": "MNKKQIKKFLGLSLTIPTLLAIPLVSLSCKKSKDNQDNLELGNDDGIEKQDLSVAKRIVEIFKEHRFSFGEMNQKLFLQNQNIEDPKKPFKKIRFEDFYNNRVKDWNFKEKSEYNRALELDNDLKYPFFDIKNQNSFINLLKNDPDPNLYRVFNKYFQIRLRTNDELLKILFVFELVSKKMQQQLSNEIKKDASDLNVKNYFNLKTYAINHFLLDYTEFLESEEPETTMHYTFRLLSYIVPPVAISSIIIYIIIMVLKKRHKSKTRA", "aa_hexdigest": "501906a46a790d53119b455127e792e9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158B1EB6", "ncbi_nrp_id": "WP_175933510.1", "uniref100_id": "UniRef100_UPI00158B1EB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B1EB6", "RefSeq:WP_175933510.1", "UniRef:UniRef100_UPI00158B1EB6" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B1EB6", "uniref90_id": "UniRef90_A0A454CA04" }, "psc": { "uniref90_id": "UniRef90_A0A454CA04", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_A0A454CA04" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA04", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA04" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "AHCKFKLKGB_723", "locus": "GCA013367975_00712" }, { "type": "cds", "sequence": "contig_1", "start": 408981, "stop": 409604, "strand": "+", "frame": 3, "gene": null, "product": "Vaa surface lipoprotein adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O53103" ], "nt": "ATGAAAAAATCAAAAAAAATATTTATAACATTATGTGGAATAGCTGCTACCGCTGTTTTGCCAGTTGCTACTATTTCATGTAATGATGATAAACTAGCAGAGAAAAATGGAAAAGAAAAAGCAGATGCTGCCTTAAAACAAGCAAATGCATTAGCAGAAGAATTGAAAAAAAACCCAGATTATTCAAAAATTTTAGAAACATTAAACAAAGAAATTGCTGAAGCAACCAAGTCATTTAAAGAAGCAGGTTCATACAGCGATTATCCAGCAATAATTTCTAAACTTTCAGCAGCAGTAGAAAATGCAAAAAGTGAACAACAAAAAGTTGATCAAGCAAATAAAAAAATTGCTGATGAAAATTTAAAAATTAAAGAAGGCGCTAAAGAATTATTGAAATTAAGCGAAAAAATTCAAAGTTTTGCTGATACAATTGCATTAACAATTACTAAATTAGAAGGAAAAAAATTCCAAATTGATGAAACATTTAAAAAACAATTAATTTCAACAATTGAATTATTAAATAAAAAATCAGCCGAAGTTAAAACTTTTGCAACAGTAAATACCATTAAAAAAGATTTCCTTTTATCAGAACTAGAATCATTTAAAGAATTTAACACCTCATGA", "aa": "MKKSKKIFITLCGIAATAVLPVATISCNDDKLAEKNGKEKADAALKQANALAEELKKNPDYSKILETLNKEIAEATKSFKEAGSYSDYPAIISKLSAAVENAKSEQQKVDQANKKIADENLKIKEGAKELLKLSEKIQSFADTIALTITKLEGKKFQIDETFKKQLISTIELLNKKSAEVKTFATVNTIKKDFLLSELESFKEFNTS", "aa_hexdigest": "2c59a5923fcd3cbd71435f69c39cc5b6", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_O69453", "query_cov": 1.0, "subject_cov": 0.8023255813953488, "identity": 0.889, "score": 327.0, "evalue": 2.25e-111, "valid": false, "product": "p50 adhesin", "uniref50_id": "UniRef50_O53103" }, "pscc": { "uniref50_id": "UniRef50_O53103", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O53103" ], "product": "Vaa surface lipoprotein adhesin" }, "genes": [], "id": "AHCKFKLKGB_724", "locus": "GCA013367975_00713" }, { "type": "cds", "sequence": "contig_1", "start": 409757, "stop": 409909, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTTACTGTTGCAAAAGTTTTAACTTCGGCTGATTTTTTATTTAATAATTCAATTGTTGAAATTAATTGTTTTTTAAATTTTTCATCAATTTGGAATTTTCTTTCTAATTTAGTAATTGTTAATGCAATTGTATCAGCAAAACTTTGA", "aa": "MVFTVAKVLTSADFLFNNSIVEINCFLNFSSIWNFLSNLVIVNAIVSAKL", "aa_hexdigest": "f04784822c3ff8ceec3fa0f124055702", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5568.5049, "isoelectric_point": 5.820676612854004 }, "id": "AHCKFKLKGB_725", "locus": "GCA013367975_00714" }, { "type": "tRNA", "sequence": "contig_1", "start": 410620, "stop": 410696, "strand": "-", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 59.6, "nt": "GCGTTTGTGGCGAAGTGGTTTAACGCTCCGGGTTGTGGCTCCGGCATACGTGGGTTCGATCCCCATCAAACGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 410688, 410690 ], "id": "AHCKFKLKGB_27", "locus": "GCA013367975_00715" }, { "type": "cds", "sequence": "contig_1", "start": 410889, "stop": 411239, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAATGCATTAGAAATAATTGAAGCAGGATATAATTCAGCATTAAAAGGAGAAAGTTTGGAAGCCTTAAAAGCAGAAACAAGCAAAATAAAAAATTTAAATGAATTAATTAAATTGTTTTATGGATATTGGAAAAAATTTAACCATTTGATTTCTGACAATCATACTAAAGAAGAAGTCTTTAAAAGAGGAATTAAAGATGCTAAAGAAGCAATAAATGCCTTGGTATCAATTAATTTAAATCAAGAAATTGGTGATGATAAATTAATGAAAGAAAAAACCAGGGAAATTGAAAATGAACTAGCAACGGCTCAATCTTTGACAGAAAGCACTTTCAAACATTATTAA", "aa": "MNNALEIIEAGYNSALKGESLEALKAETSKIKNLNELIKLFYGYWKKFNHLISDNHTKEEVFKRGIKDAKEAINALVSINLNQEIGDDKLMKEKTREIENELATAQSLTESTFKHY", "aa_hexdigest": "78f95ec950892a608a1fb9cf44b51c5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13275.912399999981, "isoelectric_point": 5.944017219543457 }, "id": "AHCKFKLKGB_726", "locus": "GCA013367975_00716" }, { "type": "cds", "sequence": "contig_1", "start": 411240, "stop": 411737, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A4828", "UniRef:UniRef90_UPI0005CA5961" ], "nt": "ATGCTTAAAATAAAAGGCGGTTTAGTTTCAGTAATTAATTCAAATATAGAGCAACTTAAAAAATTTAAGGCAAAAAAATTTAATAATAATTTTATTTCAAAACTTAATGAAAAATTAAACAGTAATATTTTTAATAATCTTTCGAAAAATATGAAATTAGCCGATCTTAAAAAAATAGAAGAAGATTCAGTAAATCTTTTAAGGCTTGTCAAATTACAAGAAAAATATGAAAATGACGATAAATTGTCATCGTCTCTGCAAAAAAAACTTGTAAAAGAATTTGAAAACATATTAGACAAAGAAACAATACCTAGTGATTCTGAAATAACTGAATTAGAAAATAAATTAAAGAAAATGATTGAAGAAGACCCAACAACGGAATTTACTACAGCATTTAGTAATCTTAAAAATGAAACAAAAAATACTAAAGAATCAATTGAAAATCTAGTTCCTGAATCAGTTAAAGAACTTGATGGATTCAAAAACCTTCAATCATGA", "aa": "MLKIKGGLVSVINSNIEQLKKFKAKKFNNNFISKLNEKLNSNIFNNLSKNMKLADLKKIEEDSVNLLRLVKLQEKYENDDKLSSSLQKKLVKEFENILDKETIPSDSEITELENKLKKMIEEDPTTEFTTAFSNLKNETKNTKESIENLVPESVKELDGFKNLQS", "aa_hexdigest": "0ad8c7cfc2200d5001ad861cff58024a", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 410937, "stop": 412037, "observations": { "mutation": [ 411242, 411749 ], "stop": [ 411242, 411749 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005CA5961", "query_cov": 1.0, "subject_cov": 0.40441176470588236, "identity": 0.867, "score": 248.0, "evalue": 8.59e-79, "gene_start": 1, "gene_end": 165, "reference_start": 128, "reference_end": 292, "reference_sequence": "AKKVLDENNANENSALEEITNATKALDEAKSKLDQEIMAKKQELMANLKEKNEKLEALLNTENLEDLMSIKGSGGLSADEIKNKLEDARKLLQEAKGLSENSKITNIKQKILKIDEFVGKLEPWISNMLKIKGSLVSVINSNIDKLKKFKAKKFNKNFISKLNEKLNSNIFNNLSRNMKLADLKKAEEDSVNLLRLVKLQEKYENDDNLSSSLQEKLVKEFENILDKETIPSDSEITELENKLEKIIEEDPTTPFTREFSYFKNETKDKKEFIENLVPQSAKELAEFKNLQSWINNALSVIEEWYNLALKNNDLDGLKTEKIRIENLNKLIKLLYFYLKGSGKLVSSHHDKEEVFRKAIKEAREALNDMASINLNQDLGNDKLMKEKIEDIENKLSTARSLTESHFKR" }, "paralog": false, "identity": 0.773224043715847, "score": 482.0, "evalue": 2.74e-173, "description": "Internal stop codon at 411242, 411749. Nonsense mutation around 411242, 411749.", "psc": { "uniref90_id": "UniRef90_UPI0005CA5961", "uniref50_id": "UniRef50_UPI00193A4828", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005CA5961", "UniRef:UniRef50_UPI00193A4828" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193A4828", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A4828" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19013.566700000007, "isoelectric_point": 7.750644493103027 }, "id": "AHCKFKLKGB_727", "locus": "GCA013367975_00717" }, { "type": "cds", "sequence": "contig_1", "start": 411747, "stop": 417398, "strand": "-", "frame": 2, "gene": null, "product": "Variable membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9Y3", "UniRef:UniRef90_Q700S8" ], "nt": "ATGAATAAGAAAATAGGTATTTTACTTTCAATTTCTTTGTTAGCCACAGCAGCTACAACTGCTACTATAGCAGCCATTGCTGCAAAAGGAAAATTTAAAAAGCAACAAGAATTAAAGCAAAATATTGAAAAATCTAAAGCCGAACTAAATAAGGCCAAATCTATTTTAGAAAAATTGTTAAAATCAGATTATGCTAAAAATGTTAACAAATTAGAAGAATCTAAAATTTTAGATAATGCTAAGATTGATGAAAATTCTTCGCTTCAAGACATTAAAAATAAAACTAAGCAAGTAAAAGAAGCAATTAATTCATTAACTCAAAAAATTCTTGAAAGAAAACAAAAAGAACAACAAAATCAAAATGATAAAAAAACTCTTGACAAACTAAGATTGGATTTTGCAAATGCCAAAGAAGAACTACAAGCATTGATTAATTCAAAAGACGGAAAATCAGTAGATACCACCAAGGCCGCTGAAGTATTAAAAAATACTAAAATAACAAATTCATCTGCTGCCTTTCAAGTTCTTCAAGCAGTTCAAACAATTAAAAAAGCTACAAAAGAATTGCAAGAAGCAATTAAATTTAAGAAGCAACAAGAATTTAATGAATTTAATAAAATAAAGCAAGAATTAACAGATTACATTGCAAACGATTTAAAGGATTTAAAATACAAAGATATTAAGGCGAAGGCAGAAAAATCAATTGAAAAAGTTAGCGATATAAACATTAATAGTTCTTTTATTAGCGATATTCAACAAGCAACTAAATTATTAATTGATGCTAAAAAACAAGCAATCTCAGAAGCAGAAAAAATTAATAAAAGTAGTGAATTAGACAAATATAAAATCGCTAAAAACAAATTGGAACTATTAATTAAAACCGAAGATGCTCAAATGATAAAAAGTGCCAACGAAAAAGCTAAAAAGATATTAGCAGAAAATACAACAAAGGATACCACATTATCATCTGAAGAATTTAAGACAAGAAGAATAAATCTTGAAAACGCTCTAGAAGAACTTAAAGAAGCAATTAAAAATGAAAAGAGTATTCAAAAACAAGAATTTGAAGCAAAAAAACAAGAATTAAAGACTTTGCTAGAATCAAAATGGTCAAAAGATATTGACAAGAAAAACGAAACAACAGTCTTAAATAATGCAAAGATTGATGATAGCGATTCAATAAAAGAAATCAAGGAAGCAACAAAAGAAGTTCAAGGTGCTATTGATTCATTGATCCAAAAAATGGATGAAAAAAATAAATTAAATCCTTCATATGCAAAAGAAAAATTTGAAAAAATTCAAAAACAATTGCAAGATTTAATCAATTCAGAAGATGCAAAATTCATTGATACTAATAAAGCATCCGAAACATTAAAAAATACTAAAATAACAAGCACTTCTTCAATAGATGAAATAAACCAAGCAATTAAAACTCTTAAAGATGCAACCGTTGAATTGAAAAATGCAATAAATGGTGCTAAAGAAGATTCAATAAAAGAATTTGATAAAGTTAAAAAAGATCTAGAAGTTTTAGTAGGCTCGGAAGATGCAAAACCAATTGAAAAAAGAACTGAAATTGGTATTTTGGCAAACACTTATATTCTCGAAAGAGATACAATAAAACAAATTAAAGAAAAGACTGAAGCAATTAAAAAAGCAATATCATCTTTAAACAAAAAAATTGAAGACGTTAAAGCTTTAAAAAACGAATTGCAAAAATTTAATGATATCAAGGTTGCTTTAGAACAATTAATTAAAACTGATGATGCAAAAGAAGTTGGAACAGAAGATGCATCAAAAGCTCTTAATAATAATAAAGTAAATGAAAATTCAACTCTTGAAGAAATAACAAAGGCAACTAAAGCACTTGAAGATGCAAAATCTAAATTAGATCAAGAAATCAAGGCTAAAAAAGAAACTGAATTTAATAATTTAACAGTTGAAAAAGGCAAATTAAATGAATTAATTGCCAAATCAACTGATGCGTCAGCAAAAGCAATTGAAGAAGCCAAAAAAGTATTGGCAGAAGTTGAAAAATTAAATAACTCTTCACCAATTAATTTACTAAAAAATGCAGTTATAAAAACAAAGGACGCTATAGAAAAATTAAATAATAATGTTGCCCAAGAAAAAGACAAAAAAGCCAAATTAGTTGAATTTAATAGTATAAAAACACAATTAGAAGAATTGATTGCAAAAGATGATGCAATTCAAGCCGGAGTAAACGAAGCTAAAAAAGCATTACAAGATAATAAAGCTGATGAAAATTCAACTCTTGAAGAAATAACAAAGGCAACCAAGGCATTGCAAGAAGCAAAAGCAGCATTGGAACTAAAAATTTCAAAGGCTCAAGAAAAAGCTAAACAAGAATTTGAAACTAAAAAAGAACAATTAAAAACATTAATTGGCTCAAGTGATGCAAATAATGTTGATAAAGATAATGAATTAAATGTTTTAACTAATACTTCAATTTCTAAAACAGATTCTATTAAAGAAATTAAACAAAAAACTTCAACTATCGATGAAGCAATTAAGACCTTAAATAAAAAAGTTCAAGATAAAAAGAACGAAAAAATTCAAGAATTTGAAAAGGCTAAAGAAAGTCTAAAAAAACTAGTTAAAGAAGAAGATGCAAAAGAAGTAGGCATTGATAGTGCAAACGAAGCTTTAAAAACAAAAGTTGATGAAAAGTTAACTATTGAAGAAATTAAAAATGCTATTTTAAAAATATCTAACGAAGAAACAAAATTAAAAAGTAGCATTGAAAGTGCAAAACAACAAGCTAAAGAAACATTTAATTCAAAGAAACAAGAATTAAAAAGCCTTTTAGATTCTACAACATCTGAAGTTGATAAAAAAGCCGAAGAAGAAAAATACAATAAAACTACAATTGATAATGATTCTACAATTAAACAAATTAAACAAAAAACAAGCGACATCGAAAATGCAATATCAACCTTAAAAGAAAAAATAGCCAAAGCAAAAGGAGATAAGGCTGTTGAATTACAAAAATTTAATCTAGTAAAACAACAATTAGAAGAATTAATTGCAAAAGAAGATGCAAAAGAAGTTGGAACTTCTAAGGCTGAAGAAGCATTGAAAGTTAATGTAGCAGACGAAAATTCAACTCTTGATGCTATTTCAAAAGCAACTAAAGCTCTTGAAGGTGCTAAAAGCGAATTAACACAAAATATTATTGATACTAAAAATAAAGAAACAAAAACATTCAATGAAAAGAAAGATGAATTAAACAAATTACTAGATACAAGTGATGCCAAAAGTGTTGATAATAAAAAAGAATCTGATGTTTTAAAAGATAATTCAATTGATTCAAATACTCCAATAAAAGAAATAAAAGCCAAAACAGAAAAAATTACAGAAGCAATTAATTCTTTGACTACAAGTATAGAAAATAAAAAGGATGAAGAATTTAAAAAATATAATGATATTAAAGCATCATTAGAAAATTTAATCAAAGAAGAAGACGCAGTTCAAGTGGGCATAGCTGATGTTCAAAAAACCTTATCCGAAAACAATGTAGATAAAACTGCAACTATTGAAAAAATTCAACATTCAACCGAAGCATTAACTCATGCTAAAGAAGAATTTCAAAAATTAATTGACACTACAAAAGAACAATTAACTAAAGAATTTGAAACTAAAAAATCTGAATTAGAAAAATTAATTTCCTTACCAGATGCAAATAATGTTGATAAAAACAAAGAATTGAGCATATTCGGCAATACCAACATTACTAATAGTGATTCTATTAAACAAATAAAAGAAAAAATAACAAAAATCCAAAATGCTCTTGAATCATTAACTAATAAAATTTCAAAGCAAAAAGAACAAGAATTAGAAAAATACAATACCACCAAAACAGCGCTAGAACAATTAATTAAAGATGAAGATGCAAAAGAAGTTGGTACAACCGATGCTAATGCAGCAATAACTAAAAACAAAGCTGATAAAAATTCAACTCTTGAAGAAATTACAAACGCAACTAAAGCACTTGAAGATGCAAAATCTAAATTAGATCAAGAAATCAAGACTAAAAAAGAAGCTGAATTTAATAATTTAACTAACGCAAAAACAGAATTAAGTAATTTAATTACTAAACAAGCACCAGCTGAAGCAATATCTAATGCTAAAAAAGTATTAGAAGAAATTAATGGTTTAAATCTAAGCAATGATTCCTCAATAAAATCATTGAAAGATGCAACTCAAAAAATTAAAGATGCAAACGAAGCATTAAAACAAGCTATAGAAAAATTAGAAACAGAAAAAACCGAAAAATTGCAAAAATTCACCGAAGCAAAAAAAGCTTTAGAAGATTTAATTAAAGATGAAGATGCAAAAGAAGTCGATACAACCGATGCTAATGCAGCAATAACTAAAAACAAAGCTGATAAAAATTCAACTCTTGAAGAAATTACAAAGGCAACTAAAGCACTTGAAGATGCAAAATCTAAATTAGAACAAAAAATCAAGGCTAAAAAAGAAGCTGAATTTAATAATTTAACTAAAGCAAAAACAGAATTAGGTGATTTAATTACCTCCTCAAGTAATCAAGCACCAGCAGATGCAATATCTAATGCTAAAAAAGTATTAGAAGAAATTAACGGTTTAAATCTAAGCAATGATTCCTCAATAAAATCATTGAAAGATGCAACTCAAAAAATTAAAGATGCAAACGAAGCATTAAAACAAGCTATAGAAAAATTAGAAACAGAAAAAACCGAAAAATTGCAAAAATTCACCGAAGCAAAAAAAGCTTTAGAAGATTTAATTAAAGATGAAGATGCAAAAGAAGTTGATACAACCGAAGCTACTGCAGCATTAACTAAAAACAAAGCTGATAAAAATTCAACTCTTGAAGAAATTACAAACGCAACTAAGGCACTTGAAGATGCAAAATCTAAATTAGAACAAAAAATCAAGGCTAAAAAAGAAGCTGAATTTAATAATTTAACTAAAGCAAAAACAGAATTAGGTGATTTAATTACCTCCTCAAGTAATCAAGCACCAGCAGATGCAATATCTAATGCTAAAAAAGTATTAGAAGAAATTAACGGTTTAAATCTAAGCAATGATTCCTCAATAAAATCATTGAAAGATGCAACTCAAAAAATTAAAGATGCAAACGAAGCATTAAAACAAGCTATAGAAAAATTAGAAACAGAAAAAACCGAAAAATTGCAAAAATTCGCCAAAGCAAAAAAAGCTTTAGAAGATTTAATTAAAGAAGAAGATGCAAAAGAAGTTGGAACAAATGATGCCCAAAAGTTGTTAGAAGACAATAACAAGATTGATAAAAATTCAACTCTTGAAGAAATTACAAAAGCAACTAAGGCACTTGAAGATGCAAAATCTAAATTAGATCAAGAAATCAAGGCTAAAAAACAAGAATTGATGGCTAATTTAAAAGAAAAAAACGAAAAATTAGAAGCGTTATTAAACACTGAAAATTTAGACGACCTTATGTCTATTGAAAAGCTTATTGGTTTAAGTGCAGGCGAAATAAAAAATAAACTTGAAGATGCTAAAAAATTATCGCAAGAAGCAAAAGGTCTTCCTGAAAATTCAAAAATTACTAATATAAACCAAAAAATTCAAAAGATAGATGAATATGTTAGACAATTTGAGCCTTGA", "aa": "MNKKIGILLSISLLATAATTATIAAIAAKGKFKKQQELKQNIEKSKAELNKAKSILEKLLKSDYAKNVNKLEESKILDNAKIDENSSLQDIKNKTKQVKEAINSLTQKILERKQKEQQNQNDKKTLDKLRLDFANAKEELQALINSKDGKSVDTTKAAEVLKNTKITNSSAAFQVLQAVQTIKKATKELQEAIKFKKQQEFNEFNKIKQELTDYIANDLKDLKYKDIKAKAEKSIEKVSDININSSFISDIQQATKLLIDAKKQAISEAEKINKSSELDKYKIAKNKLELLIKTEDAQMIKSANEKAKKILAENTTKDTTLSSEEFKTRRINLENALEELKEAIKNEKSIQKQEFEAKKQELKTLLESKWSKDIDKKNETTVLNNAKIDDSDSIKEIKEATKEVQGAIDSLIQKMDEKNKLNPSYAKEKFEKIQKQLQDLINSEDAKFIDTNKASETLKNTKITSTSSIDEINQAIKTLKDATVELKNAINGAKEDSIKEFDKVKKDLEVLVGSEDAKPIEKRTEIGILANTYILERDTIKQIKEKTEAIKKAISSLNKKIEDVKALKNELQKFNDIKVALEQLIKTDDAKEVGTEDASKALNNNKVNENSTLEEITKATKALEDAKSKLDQEIKAKKETEFNNLTVEKGKLNELIAKSTDASAKAIEEAKKVLAEVEKLNNSSPINLLKNAVIKTKDAIEKLNNNVAQEKDKKAKLVEFNSIKTQLEELIAKDDAIQAGVNEAKKALQDNKADENSTLEEITKATKALQEAKAALELKISKAQEKAKQEFETKKEQLKTLIGSSDANNVDKDNELNVLTNTSISKTDSIKEIKQKTSTIDEAIKTLNKKVQDKKNEKIQEFEKAKESLKKLVKEEDAKEVGIDSANEALKTKVDEKLTIEEIKNAILKISNEETKLKSSIESAKQQAKETFNSKKQELKSLLDSTTSEVDKKAEEEKYNKTTIDNDSTIKQIKQKTSDIENAISTLKEKIAKAKGDKAVELQKFNLVKQQLEELIAKEDAKEVGTSKAEEALKVNVADENSTLDAISKATKALEGAKSELTQNIIDTKNKETKTFNEKKDELNKLLDTSDAKSVDNKKESDVLKDNSIDSNTPIKEIKAKTEKITEAINSLTTSIENKKDEEFKKYNDIKASLENLIKEEDAVQVGIADVQKTLSENNVDKTATIEKIQHSTEALTHAKEEFQKLIDTTKEQLTKEFETKKSELEKLISLPDANNVDKNKELSIFGNTNITNSDSIKQIKEKITKIQNALESLTNKISKQKEQELEKYNTTKTALEQLIKDEDAKEVGTTDANAAITKNKADKNSTLEEITNATKALEDAKSKLDQEIKTKKEAEFNNLTNAKTELSNLITKQAPAEAISNAKKVLEEINGLNLSNDSSIKSLKDATQKIKDANEALKQAIEKLETEKTEKLQKFTEAKKALEDLIKDEDAKEVDTTDANAAITKNKADKNSTLEEITKATKALEDAKSKLEQKIKAKKEAEFNNLTKAKTELGDLITSSSNQAPADAISNAKKVLEEINGLNLSNDSSIKSLKDATQKIKDANEALKQAIEKLETEKTEKLQKFTEAKKALEDLIKDEDAKEVDTTEATAALTKNKADKNSTLEEITNATKALEDAKSKLEQKIKAKKEAEFNNLTKAKTELGDLITSSSNQAPADAISNAKKVLEEINGLNLSNDSSIKSLKDATQKIKDANEALKQAIEKLETEKTEKLQKFAKAKKALEDLIKEEDAKEVGTNDAQKLLEDNNKIDKNSTLEEITKATKALEDAKSKLDQEIKAKKQELMANLKEKNEKLEALLNTENLDDLMSIEKLIGLSAGEIKNKLEDAKKLSQEAKGLPENSKITNINQKIQKIDEYVRQFEP", "aa_hexdigest": "672c5f4d1cd15ac425a489e2cd7460a9", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_Q700S8", "query_cov": 0.9840679766330324, "subject_cov": 0.8543107422775472, "identity": 1.0, "score": 2896.0, "evalue": 0.0, "valid": true, "product": "Variable membrane protein", "uniref50_id": "UniRef50_A0A454C9Y3" }, "pscc": { "uniref50_id": "UniRef50_A0A454C9Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9Y3" ], "product": "Variable membrane protein" }, "genes": [], "id": "AHCKFKLKGB_728", "locus": "GCA013367975_00718" }, { "type": "cds", "sequence": "contig_1", "start": 417767, "stop": 418087, "strand": "-", "frame": 3, "gene": "hinT", "product": "histidine triad protein HinT", "db_xrefs": [ "COG:COG0537", "COG:FR", "RefSeq:WP_012855622.1", "SO:0001217", "UniParc:UPI0001BC232C", "UniRef:UniRef100_D1J8D6", "UniRef:UniRef50_P32083", "UniRef:UniRef90_D1J8D6" ], "nt": "ATGCATGATGATATTTTTGAAAAAATAATAACAAGAGAAATCCCAGCAAAAATAATTTATGAAGATGAAAAATTTATTGCCTTTGAAGATATTAAACCAGTAGATAAGGGCCATTTTTTAGTAACGGTAAAAAACCACGCCACGAATTTATATGATATTGATGACCAAGCATTGTCATCACTAATATTAAAGGCGAGAGAACTTGCTCTAAAAAGAGTTAAGGAATTAGGCGCAACTGGATTTAATTTAATAATAAATAATAATCTTTCAGCAGATCAAACAGTATTCAGAATTCATGTTCATATAATTCCAAGATATTAA", "aa": "MHDDIFEKIITREIPAKIIYEDEKFIAFEDIKPVDKGHFLVTVKNHATNLYDIDDQALSSLILKARELALKRVKELGATGFNLIINNNLSADQTVFRIHVHIIPRY", "aa_hexdigest": "032e4d9f517db52ca0cbb90f5dbef90e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC232C", "ncbi_nrp_id": "WP_012855622.1", "uniref100_id": "UniRef100_D1J8D6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC232C", "RefSeq:WP_012855622.1", "UniRef:UniRef100_D1J8D6" ] }, "ips": { "uniref100_id": "UniRef100_D1J8D6", "uniref90_id": "UniRef90_D1J8D6" }, "psc": { "uniref90_id": "UniRef90_D1J8D6", "gene": "hinT", "product": "histidine triad protein HinT", "uniref50_id": "UniRef50_P32083", "cog_id": "COG0537", "cog_category": "FR" }, "pscc": { "uniref50_id": "UniRef50_P32083", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P32083" ], "product": "Uncharacterized 13.1 kDa HIT-like protein in P37 5'region" }, "genes": [ "hinT" ], "id": "AHCKFKLKGB_729", "locus": "GCA013367975_00719" }, { "type": "cds", "sequence": "contig_1", "start": 418089, "stop": 418217, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTGCTAATTTTGAGTCATTATATAAAACTGCAGTTAAATATATTAATGAACTAGGGTTTAAATTAAATGTTTCAGAAACAAACAAATCTGTTTATGATATATTGAAAGTTGAAGGTCTAGTATAA", "aa": "MTANFESLYKTAVKYINELGFKLNVSETNKSVYDILKVEGLV", "aa_hexdigest": "bbfeebaf8b194e99c537736287b7ff2e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4770.4540000000015, "isoelectric_point": 6.0701997756958 }, "id": "AHCKFKLKGB_730", "locus": "GCA013367975_00720" }, { "type": "cds", "sequence": "contig_1", "start": 418257, "stop": 418952, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGGCGAAGTATTAAAATCTTTATTGGCATATAAAGGCATTGTTAAAAATTCAAATACGTCAGGTGATTTAGGCGGCAATTTAGATAGTATAAAGAAAGATTTAAGTTCAGTATATGGATTTGTAGATCCATTTAGTAAAAAAGTTTATAGTCAAGAAAGCTTTTTACTTGCAAAAATTCTTGCCCAAGAAATAAATCATCCAAAGGCAAAAGCAACCGAAACATTGCAAACTAAAGCTTCAAAAGGTGAATTAAAATCGTTTGATTACAAAGATTACGAATTTGACGGTTTAACAAAAGATAGCAAGGATAATTATCAATATACAAAGACAATAACTCTTGGTAGTAAGCAATATACATTGAGATTTAGCCAAAAAGGTTCAATATCATTTGATGGTAACTTTTTAACAATTCCAATGAATTTAACAGTTGATGGTTTAGGAAAAAGAAATTTCTATGAATTCAATGCTAAACTAGAATACAATAAAAGTACAAAGAAATTTGAGTCAATAAGTCAAAATGTTGCATACAATTTAAAACAAAATCCTCAAAGAATAAATGTTACAAAAGACAATTCAATTACTGCCCAATATGTAGTTAAAATTTCACCATTATATTTAACTAAGAAAGTTAAAGATTATAACGGCAAAGAAGTTACAAAACAAGTTTTAACATTTGATGAAACTCCATGA", "aa": "MEGEVLKSLLAYKGIVKNSNTSGDLGGNLDSIKKDLSSVYGFVDPFSKKVYSQESFLLAKILAQEINHPKAKATETLQTKASKGELKSFDYKDYEFDGLTKDSKDNYQYTKTITLGSKQYTLRFSQKGSISFDGNFLTIPMNLTVDGLGKRNFYEFNAKLEYNKSTKKFESISQNVAYNLKQNPQRINVTKDNSITAQYVVKISPLYLTKKVKDYNGKEVTKQVLTFDETP", "aa_hexdigest": "abab5f60e25e350a91e89ab3646a5dfb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26126.289900000014, "isoelectric_point": 9.32324047088623 }, "id": "AHCKFKLKGB_731", "locus": "GCA013367975_00721" }, { "type": "cds", "sequence": "contig_1", "start": 419115, "stop": 419471, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATCAATGCAAAGATTGATACAACTAAATATAATCCCGCTGCATTTTCAATACCAAGTGAACAAGAATTTAAATTTATTTTTAAACATTCAAGTCAAATCGCAGCAAACATTAGATTAGAACTTCAAAAAATGTTACTTGCTAAACTTTATTTATTAAAAAATAGACCAGAACTTAAAAAAATAGCAAATGATAGCAACGGTCTAGACAAAGCACAAGTTGCATTACACAACAAAATGTCTAAGAAAGATGCTCCTATTAATGAAAAAGAATTATACGAAGCATTGAATTTTGCAGATGATTCATTGTATTTAATGAAATATCTAGTTGAAAATCCGATTATTGAAAATTGA", "aa": "MFINAKIDTTKYNPAAFSIPSEQEFKFIFKHSSQIAANIRLELQKMLLAKLYLLKNRPELKKIANDSNGLDKAQVALHNKMSKKDAPINEKELYEALNFADDSLYLMKYLVENPIIEN", "aa_hexdigest": "10c9a1aa9897d14e41528f44b6825624", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13605.65139999998, "isoelectric_point": 8.730904960632323 }, "id": "AHCKFKLKGB_732", "locus": "GCA013367975_00722" }, { "type": "cds", "sequence": "contig_1", "start": 419673, "stop": 419837, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAAAAAAATTAGATTATTAACAGCTATTGCAATGCCACTTGCGGTTCTTCCTTTGATTGCTAGTTCATGTAAAAAAGAAAAGGAAGATTCTCAACAAAATTCAGGGTACCAAAAACGTGTTTTAAAAGATTCATTAACAAAAAATAGGGTTTTAACTTGA", "aa": "MTKKIRLLTAIAMPLAVLPLIASSCKKEKEDSQQNSGYQKRVLKDSLTKNRVLT", "aa_hexdigest": "96c489145654e415ec9ec61e1a5af3c0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6059.176500000003, "isoelectric_point": 10.103952598571777 }, "id": "AHCKFKLKGB_733", "locus": "GCA013367975_00723" }, { "type": "cds", "sequence": "contig_1", "start": 419849, "stop": 420973, "strand": "-", "frame": 3, "gene": null, "product": "HinT-interacting membrane complex protein P80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BDBC09" ], "nt": "TTGAATGAAAGTGATGACAAGGTTTCAATCAAACAAGGCATTGATGCCATTTCAACATTTAAGGGCATATCTTCACTTCTTGAAACAAATGAAATTACTAAAAATCAAGAAATTGCAGCCAAAAATGATAGCAACCTTTTGAGATATGTTTCAAGCGATAGCAAGTCAGCTGATAAATACGGTTTCGACGGCTTTAAAAGTGTGTTGAATAGTTTAAAAGAAAAAGATCCTAATAAGTATATGAATCTTCTTTCAATCTTAATGGGCGATGCTGACAAAGATAAAAGCATTTTTAAAGCTGAAGATAAAAAAGATTTGTTTAAAAAATTAAAAGAAAACTTCAAAAAGATCTTTGGCGATGCTACATTTATTGATCATAAAAAGAACACAATGAAATTTGCTGATATTTTTAATGAAGGAACTGAAAGTGTTGACAAAGAAAACTTCTCTGAAAAATACACTAGATACAATGCAGCTATTGAAAAATTTATCAATGATTTAAGTGAAAAAGATTTTAATAAATCTTTTGGCTTGGCATTTAGAGATGCATTTAGTGATGAAAAAAGTGGATATAAGGTTTCAACATTAATTAAAAGTAATGGAAATTACATTCAAGTCAATGAAAGTGGAATTTTGATTCAAAATGTATTTTCATTAGACAAAGAAGAAGTTGTAACTAGATTGATAACAAATGATTTGTCAATACTATCTAAGGCCAACCACTCAGATACATTGAATACTAAATTATTTAATTTAGATTCAATATTTTCTGAAATTTTAACTCGTGACTATATGGTTAATGATTTATTACAACAAGATAATTTTAAAAATTATATTAAATCAAAAGAATATTTAAGTCTTGAAGGAACAAAACGTAAATTCAATGATCAAGATATTTTAAATGCAATCAACTATGAAAAATTAATTCGTGAAACAAGCAAATACGTTTTAATTAATGGCAAAGCAAACGAAATAAAACAATTTATAATTAAAAAATTTAATAGTAATTTATACAAAGATTATGTGTTCAAAGACAATAAGTTTATTCTATCGCCCCATGAAGACAAAGAGATCTTATCTTATTTATTCACAACAATTGTAAATTACATAAATACTAAAAATTAA", "aa": "MNESDDKVSIKQGIDAISTFKGISSLLETNEITKNQEIAAKNDSNLLRYVSSDSKSADKYGFDGFKSVLNSLKEKDPNKYMNLLSILMGDADKDKSIFKAEDKKDLFKKLKENFKKIFGDATFIDHKKNTMKFADIFNEGTESVDKENFSEKYTRYNAAIEKFINDLSEKDFNKSFGLAFRDAFSDEKSGYKVSTLIKSNGNYIQVNESGILIQNVFSLDKEEVVTRLITNDLSILSKANHSDTLNTKLFNLDSIFSEILTRDYMVNDLLQQDNFKNYIKSKEYLSLEGTKRKFNDQDILNAINYEKLIRETSKYVLINGKANEIKQFIIKKFNSNLYKDYVFKDNKFILSPHEDKEILSYLFTTIVNYINTKN", "aa_hexdigest": "0abb2432ed60ee4e8797aca97573e7c1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BDBC09", "query_cov": 0.8155080213903744, "subject_cov": 0.9967320261437909, "identity": 0.518, "score": 288.0, "evalue": 2.24e-92, "valid": false, "product": "HinT-interacting membrane complex protein P80", "uniref50_id": "UniRef50_UPI0021BDBC09" }, "pscc": { "uniref50_id": "UniRef50_UPI0021BDBC09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BDBC09" ], "product": "HinT-interacting membrane complex protein P80" }, "genes": [], "id": "AHCKFKLKGB_734", "locus": "GCA013367975_00724" }, { "type": "cds", "sequence": "contig_1", "start": 422091, "stop": 422504, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2617A5", "UniRef:UniRef90_UPI001F2617A5" ], "nt": "ATGAATAAATTACTTTTAAATAAGGATAGAATATTGCTTGAATTTATCGAAATTTGTAAAAATCTTAATCTTTTTTATAGTTTAGATAAATTGAGCTTGTATGGCACTATGTCAAAAGAACATTCTTGGAATAATTTGGATCACCACGAAATAATGATGAAATATCAAGATTATGAAATCTTAAAAAATGCTTGTCCTAATAGAGTTTTAGATAATACATTACATTCTTTGTATCATGACTTACAAATAAAATTTGTTGATGATAACAAAAATATATTTGAAAACCAACCGTTTTTGAACATTAACTTAATTTATCCAACAAACATTGCCAATATAAAGAAATATTTAAAACATTGCAATCAAGCTAGATCGTTTATTGGCCACTATTTAACAATGACAAATAGCAATGATTGA", "aa": "MNKLLLNKDRILLEFIEICKNLNLFYSLDKLSLYGTMSKEHSWNNLDHHEIMMKYQDYEILKNACPNRVLDNTLHSLYHDLQIKFVDDNKNIFENQPFLNINLIYPTNIANIKKYLKHCNQARSFIGHYLTMTNSND", "aa_hexdigest": "9f7bd439fe3090507d0c8493f42a3227", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 422091, "stop": 422616, "observations": { "insertion": [ 422592 ], "mutation": [ 422502 ], "stop": [ 422502 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2617A5", "query_cov": 1.0, "subject_cov": 0.7873563218390804, "identity": 0.993, "score": 280.0, "evalue": 3.99e-95, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MNKLLLNKDRILLEFIEICKNLNLFYSLDKLSLYGTMSKEHSWNNLDHHEIMMKYQDYEILKNACPNRVLDNTLHSLYHDLQIKFVDDNKNIFENQPFLNINLIYPTSIANIKKYLKHCNQARSFIGHYLTMTNSNDWKTKNNIIKSHFLSMFYKPITYKEVANLLIKMITKVF" }, "paralog": false, "identity": 0.9827586206896551, "score": 348.0, "evalue": 2.7e-124, "description": "Internal stop codon at 422502. Frameshift due to insertion around 422592. Nonsense mutation around 422502.", "psc": { "uniref90_id": "UniRef90_UPI001F2617A5", "uniref50_id": "UniRef50_UPI001F2617A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2617A5", "UniRef:UniRef50_UPI001F2617A5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2617A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2617A5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16368.646599999982, "isoelectric_point": 7.045727348327636 }, "id": "AHCKFKLKGB_735", "locus": "GCA013367975_00725" }, { "type": "cds", "sequence": "contig_1", "start": 422658, "stop": 422837, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F176BBD", "UniRef:UniRef90_UPI001F176BBD" ], "nt": "TTGCCAAATATTAAATTTGAAACAAAAAAAGTAGTTATAAATAATATTGAAGTTGATGTCTTAGTTGAATTTGACCAATATCTAAAAAACGTTTATGAAGTTGAATATTTTGAACATCCCATTCCACAAAGAAAGATGGAATATATTAATCCCATTGAATATAAAAAAATATTTAGCTAA", "aa": "MPNIKFETKKVVINNIEVDVLVEFDQYLKNVYEVEYFEHPIPQRKMEYINPIEYKKIFS", "aa_hexdigest": "03750b9143f48d9727234b60258d5307", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F176BBD", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.932, "score": 114.0, "evalue": 2.3e-32, "valid": true, "uniref50_id": "UniRef50_UPI001F176BBD" }, "pscc": { "uniref50_id": "UniRef50_UPI001F176BBD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F176BBD" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7254.3351999999995, "isoelectric_point": 5.270873069763183 }, "id": "AHCKFKLKGB_736", "locus": "GCA013367975_00726" }, { "type": "cds", "sequence": "contig_1", "start": 422996, "stop": 423940, "strand": "-", "frame": 3, "gene": "lolD", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1136", "COG:M", "SO:0001217", "UniRef:UniRef50_A0A448ZXQ2", "UniRef:UniRef90_D1J8E0" ], "nt": "ATGGCAAAAGAAATAAAAGAAAACAAAAAAGAAATTAAAGCTAAAACAAATAAAATTTCTAAGAATAAAAATTTTGTTGATGAACAAACATTGCAAGATTTGAACTTGATAAATATTTCTAAGCAAAAAAAGCCATTTGAAATTAAAGATGAAGGCATTGCAATTAAGGTTTTAGATAATGCAACTTTAAAGAAATATAGACTTGCTAATGACAAACCAAGAAAAAAAGACGAGTATGCTAAATTACAAAATTCTAACGATAACATTGTTGAAGTTGATAATATTAAAAAAACATATTTATCTGGAAATGTAGCAACTGAAGTTTTAAAAGGAATAACTTTTTCAATTAAAAAAGGAGAAATTGTTGTTCTATATGGAAAGTCTGGGTCAGGTAAATCAACATTATTAAACATAATTAGTGCTCTTGACCGACCTACCTCTGGCAATGTAATTGTAAATAACATAAATTTGCCTTATTTATCTGATGCAAAGCAAACCTTATTTAGAAGAGAAAACATTTCGTTCATTTTTCAAAATTATAATTTACTTCAAAATTTGAATAGTTATGACAATGTTGAAACTGGTTCATATTTGCAAAGAGATAAAAATAAGCGTTTAAATATTAAAAAATTATTTGAAGATTTTGATCTTTCTGAATGTATGTATAAATATCCTTCTCAAATGTCTGGTGGTCAACAACAAAGAGTGTCAATTTTAAGAGCTATTGCAAAAAATTCCGAAATTCTTGTAGCTGATGAACCAACAGGAGCATTGGATGAAAAAACTGGAATTATTGTATTGAAAAATTTGCAACAAATCAATTTAAAATATGGCACAACCATTATCATTGTTTCCCACGACCCTGATGTTGCCCAAATTGCTGATAAAGTTATATACTTGGAGTTGGGATTAATTAAAGAAGTAAAACTTCAAGAAAGAAAATGA", "aa": "MAKEIKENKKEIKAKTNKISKNKNFVDEQTLQDLNLINISKQKKPFEIKDEGIAIKVLDNATLKKYRLANDKPRKKDEYAKLQNSNDNIVEVDNIKKTYLSGNVATEVLKGITFSIKKGEIVVLYGKSGSGKSTLLNIISALDRPTSGNVIVNNINLPYLSDAKQTLFRRENISFIFQNYNLLQNLNSYDNVETGSYLQRDKNKRLNIKKLFEDFDLSECMYKYPSQMSGGQQQRVSILRAIAKNSEILVADEPTGALDEKTGIIVLKNLQQINLKYGTTIIIVSHDPDVAQIADKVIYLELGLIKEVKLQERK", "aa_hexdigest": "662dd1bababf85e6d39c7f1fafb15a6c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8E0", "query_cov": 1.0, "subject_cov": 0.9721362229102167, "identity": 0.997, "score": 587.0, "evalue": 5.27e-211, "valid": true, "gene": "LolD", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A448ZXQ2", "cog_id": "COG1136", "cog_category": "M" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ2" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "lolD" ], "id": "AHCKFKLKGB_737", "locus": "GCA013367975_00727" }, { "type": "cds", "sequence": "contig_1", "start": 423990, "stop": 424670, "strand": "-", "frame": 2, "gene": null, "product": "FtsX-like permease family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16D702", "UniRef:UniRef90_UPI001F16D702" ], "nt": "ATGTTTATAAGCAATCCAAAATTAAAATATATTTCTGTATATGAACATAATATTCAATCATATAATGAAGCACATCCTCAGGCAAAATTAGATTATAAAACTCAATTAAAGAAATTACTTGGAAATCTAAACGATGAAAGTATACAAGAAATAATAGACTCAGACAGTCCATTAGAAAGTTATAAAATCTTAGTAAGAGAAACATTAACAAAATTCTATGGCACACAAGCATCTTCAATATATGGCAAGAATATAATGTATGGTGCATCATTTGATGTTAATAGCAAAGATATTGAAGCTGGATTTATTGAAGGAATTTCTTCAACAGTTAATACCATATTGCTGGCATTTATAATAATTTCATTGATAATTTCAATAGTTATATTAATTGTTATTACTAATATAATGATTGCTTCGAATGGAAGATCAATTGCTACATTTTCTATATTGGGATATACAAATAAAGAAAAAATTCTATTATTCTTCTTTAATTTTGTACCTGCAATTGTATTTGCTTGCTTACTAATGATTCCTGCAACTCTTGCAATAATAGCAATATTCAACTCGTTTATGATGGTAACTAGCCAGATTGTATTACCTTTGGTTTTACATATTTCAACAGTAATATTAAGTGCATTAATTTGTTTGGCAGTATTTATAGCAACATCAATTGCAACATGA", "aa": "MFISNPKLKYISVYEHNIQSYNEAHPQAKLDYKTQLKKLLGNLNDESIQEIIDSDSPLESYKILVRETLTKFYGTQASSIYGKNIMYGASFDVNSKDIEAGFIEGISSTVNTILLAFIIISLIISIVILIVITNIMIASNGRSIATFSILGYTNKEKILLFFFNFVPAIVFACLLMIPATLAIIAIFNSFMMVTSQIVLPLVLHISTVILSALICLAVFIATSIAT", "aa_hexdigest": "eca63e3e956efbfb1658aec33baa0952", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 423942, "stop": 424805, "observations": { "mutation": [ 423992, 424688, 424739 ], "stop": [ 423992, 424688, 424739 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F16D702", "query_cov": 1.0, "subject_cov": 0.7874564459930313, "identity": 0.996, "score": 385.0, "evalue": 1.95e-133, "gene_start": 1, "gene_end": 226, "reference_start": 46, "reference_end": 271, "reference_sequence": "MSIDKVPVQTIDTLTTYSATGFWGAASSYDVAEANDNSAWDFFKRMFISNPKLKYISVYEHNIQSYNEAHPQAKLDYKTQLKKLLGNLNDENIQEIIDSDSPLESYKILVRETLTKFYGTQASSIYGKNIMYGASFDVNSKDIEAGFIEGISSTVNTILLAFIIISLIISIVILIVITNIMIASNGRSIATFSILGYTNKEKILLFFFNFVPAIVFACLLMIPATLAIIAIFNSFMMVTSQIVLPLVLHISTVILSALICLAVFIATSIATWNSLNKIKAVDALKGK" }, "paralog": false, "identity": 0.9825783972125436, "score": 513.0, "evalue": 1.73e-186, "description": "Internal stop codon at 423992, 424688, 424739. Nonsense mutation around 423992, 424688, 424739.", "psc": { "uniref90_id": "UniRef90_UPI001F16D702", "product": "FtsX-like permease family protein", "uniref50_id": "UniRef50_UPI001F16D702", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F16D702", "UniRef:UniRef50_UPI001F16D702" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F16D702", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16D702" ], "product": "FtsX-like permease family protein" } }, "id": "AHCKFKLKGB_738", "locus": "GCA013367975_00728" }, { "type": "cds", "sequence": "contig_1", "start": 424737, "stop": 425240, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTGCAAGATAGTCGAGGCAATAATTTAACAAAATTATTACAAGACTATAATTACAATTTTGATAGTAATGATCCAATTCCTGTTGTTGTAAATGAAGTAACAGCAAGAAGCTATAGATTAAAAGTTAATTCCGTATTTGATGCCAAAATATTAAATCACGTTGATAGATTTGCATATCAAGCATTAATGCAAAAAAGTCCAAGAGTTGATTATAAGTTTAAAGTAATTGGCATATCAGATACTTATATTAACTCTGAAATTAGTACAAGAAAAGATATTTTAGACCACATTTTAGGCTATGATACTCTTTCAAAAAGATTAAAAGATGCAAGAAAATATGAATTGCAAAAAGCAATTAGTTTACACCCTGATTTAAAAGATGAATATATTAAAAAATTCAATAAAAAATATGATGCATTTAATGGTATTTTATCAATTGATAAAGTTCCAGTTCAAACTATTGATACATTAACAACTTATTCAGCAACAGGATTCTGA", "aa": "MKLQDSRGNNLTKLLQDYNYNFDSNDPIPVVVNEVTARSYRLKVNSVFDAKILNHVDRFAYQALMQKSPRVDYKFKVIGISDTYINSEISTRKDILDHILGYDTLSKRLKDARKYELQKAISLHPDLKDEYIKKFNKKYDAFNGILSIDKVPVQTIDTLTTYSATGF", "aa_hexdigest": "c8b5300a642c6e825d1fad08f957edae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19337.8133, "isoelectric_point": 9.203522682189941 }, "id": "AHCKFKLKGB_739", "locus": "GCA013367975_00729" }, { "type": "cds", "sequence": "contig_1", "start": 425475, "stop": 425783, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCGAAACACAAAGGGCAAGAGTAAACCAAATATTTAACCGCGTAAGTTCAATGATGCAATCAACTCAAAACTTAATAAATTTACGTAGATTGAAAAATGGTGGAGATTATAGAATAATAGATGAAGAAGATGTAAAAGATCCAACAAAAATAATTGCTGTAAAAGATATTAAAAAATTCTTTGATGACTATAATGCTAAACGTCCAGAAGATTTATCAAATAGAACAAGTTACTTCTTGTTTAGCGCTGGTAATTATTCAGAAGCAGCATCAGGAAATAGAAAACAATTTAGATTTGTTGAATGA", "aa": "MPETQRARVNQIFNRVSSMMQSTQNLINLRRLKNGGDYRIIDEEDVKDPTKIIAVKDIKKFFDDYNAKRPEDLSNRTSYFLFSAGNYSEAASGNRKQFRFVE", "aa_hexdigest": "6b08e1a730bc0aa42ee5b39d2731b77b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11945.299099999987, "isoelectric_point": 9.51716136932373 }, "id": "AHCKFKLKGB_740", "locus": "GCA013367975_00730" }, { "type": "cds", "sequence": "contig_1", "start": 425802, "stop": 426188, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTCGTTATTCTTAGCATTTTCAACGACTTCATTAATTTCTATGTTCTTCTTATCCTCAAATAATGTATTTAATAAAACTATTTCAAAAACATATAAAAATAGGCTATATAAATATAAATTAAATCTTGAAAGTCCAACAACCGAAGGTGGTCCTTATGTTACATACAATCAAAACGATATAAATGATTTATTGTATGTTCCAAATGATCTTGCAGGTGATTCATCAACCAATGGTAGTCAACTAGATTATGATAATCCTAATTTCTTAAGACCTGGCGCCTCATTTAATACTGATGTAATTCAAAGAAAATTTGCGCCTACTGTCATTACAAAATCCTCACTTGATTTATTAATGGATTTATCAGTTGAATTAAGTCCTTGA", "aa": "MFSLFLAFSTTSLISMFFLSSNNVFNKTISKTYKNRLYKYKLNLESPTTEGGPYVTYNQNDINDLLYVPNDLAGDSSTNGSQLDYDNPNFLRPGASFNTDVIQRKFAPTVITKSSLDLLMDLSVELSP", "aa_hexdigest": "81bfa1e11e916d49b86f73582905571f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14374.00049999998, "isoelectric_point": 4.882492256164551 }, "id": "AHCKFKLKGB_741", "locus": "GCA013367975_00731" }, { "type": "cds", "sequence": "contig_1", "start": 426192, "stop": 426377, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTTGTTTCACTAGTTATTTATATAATTACTAGAGTTATTGTAAGAAATAAACCTACAGAATTAATGAGTGGGTTAACTGAAGTTAGTGTTGGTAATTTTGCCCAAAGAGTTTCTGCTATGTTTAGAAAAATGCCAGTTAAAGCAAGATTTATTGCCTCAATGGCATTGAATAATTTCTGA", "aa": "MIVVSLVIYIITRVIVRNKPTELMSGLTEVSVGNFAQRVSAMFRKMPVKARFIASMALNNF", "aa_hexdigest": "ec87560bdd6cd360c1edb573737bef73", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6860.252400000002, "isoelectric_point": 11.466427421569826 }, "id": "AHCKFKLKGB_742", "locus": "GCA013367975_00732" }, { "type": "cds", "sequence": "contig_1", "start": 426528, "stop": 427994, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTTGCTCACGCACATACAGCAAAAGCAACAAAACAAATTATGGATTTAATGAATAATTCAGCAGTATTTAAAAATTTATTTGACCAATTATCTAAAAATCATATTTCTAATAAGGGCATTGAAGCAATTAAAGAGATATTAAAAAATAATATGAATGAAATAACCTACAATTTTGGTTATATGGCCTCTTCAAATCAAATGCCAACTCATTATCTTCAAGCCTTAAAATTATTTTTACAATCATTTATTAATGTCAATGATCAAACTCAAAAAATTAAACCGTTAATAAATGATGAATATACCTTTAGCTTAGTATATAAGGATGCAATTGCAGCATCAAAAACCGCCTCAATAATTTCGCTATTAAACGTACCAAGAAATATTCTAAATGGTTTCAATGTTTTAAGTTTCCCACAAATATTGTTATACTACGTATTAAGTCCAAACCCAGATAGTGGAAATATTGCTTATATAATCAAAAAATTCTTATCAAATATTGATGAAACTAATGCAACAATGTTGAAAAGTCAAATCGAAGGTGCAACTCAAAAATTCAATTTTGTTGAAAATGTTGTTACTTCAAAAAGCGATAGCAGAATTGATTTAGATATTTCTAAATTCAATTATATGTTTAACCATTTACTAAAAACACCCGATAACAAAGATATTAGCTTTTTTGGAATAAATATTACCAAAACTGTTAAGGAAGTATTTTCAAAAATAATAGAGCCAAGCACAGTTTATAATTTAATTGCATATTCCGAAGCTGCTTCATATTTAGCCAAGGTAAATTATGGATATTTAAACAAAAATAAAAAAGAAGTATACAAAGGCGATATTTCGAAATATTTAAATAATCCATTTGAAATGCAACAATTCATTGCTTCATTAGATGATAAATATAAAATTAAAGTAAATACCCAAGAATATTTGATAACAGGTGTTGATAGTACCGCTGATTATTTATATCCTGTAATTAATGAAGAAAACTTACAAGTTGATACAAAAACTCAATCTGTTGTTTATGTTAATTCAAGAGGTTTTGACAGAATTAGATCGGCATATCCTACATTTGCCTTAAAGAGTTATGCATTAGTAAAATCTCCTGTGGACAAAAAAGGAAACTATTTAGCTGGCAAAGATCCTAAAAACTTAAAGAAAGAACTTCAAAGAATTGTTTCATCAATTTCTCCAAATTCAAAAAATAAAGTATTTTTAAAAGATGAACCAGATGCAATCAACCCTGAACGTTACATCAGAGTGAAAACAATAAGAACATTAGTAACATCAATTAAAAATGCAACATTTTATTTAATAACAATTTTGGCAATATTAGTAATTTTGATTGTTTACTTTATTATCAAAAGATATATTGAAGCAAGAAATAAGGTTGTTGGAATATTGAGAGCTCAAGGATATAAAACATCAGAAATCGCTTTATCATTCTGTGCTTTTGGTTGA", "aa": "MPFAHAHTAKATKQIMDLMNNSAVFKNLFDQLSKNHISNKGIEAIKEILKNNMNEITYNFGYMASSNQMPTHYLQALKLFLQSFINVNDQTQKIKPLINDEYTFSLVYKDAIAASKTASIISLLNVPRNILNGFNVLSFPQILLYYVLSPNPDSGNIAYIIKKFLSNIDETNATMLKSQIEGATQKFNFVENVVTSKSDSRIDLDISKFNYMFNHLLKTPDNKDISFFGINITKTVKEVFSKIIEPSTVYNLIAYSEAASYLAKVNYGYLNKNKKEVYKGDISKYLNNPFEMQQFIASLDDKYKIKVNTQEYLITGVDSTADYLYPVINEENLQVDTKTQSVVYVNSRGFDRIRSAYPTFALKSYALVKSPVDKKGNYLAGKDPKNLKKELQRIVSSISPNSKNKVFLKDEPDAINPERYIRVKTIRTLVTSIKNATFYLITILAILVILIVYFIIKRYIEARNKVVGILRAQGYKTSEIALSFCAFG", "aa_hexdigest": "cf7af143374dfacc66a7fffc73f54b7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 55519.43329999996, "isoelectric_point": 9.420200920104982 }, "id": "AHCKFKLKGB_743", "locus": "GCA013367975_00733" }, { "type": "cds", "sequence": "contig_1", "start": 428262, "stop": 429056, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGCAACTAGTTAAACATGAACATTATCCAATTGTTGCAAATAATAAAATTTATAAAGATTTCAACACAGAAAGTTTAACTAGCTCAGATTATTTAACATCATTTTTTGGCGCAATAAGTTCAAACATAGATGGAAAAATAAATAGCGGAAAAATTTCCCAAATTCAAGAATCCGTTATTCAAATGTTTAATTTTTCAAATAAGACAAAGAGAATTTTGGGTAAATTGAATATAACTATTCCTGACTATGACAATAATAAAATTACTTTGTTTGATTTAATAAACATGTCAAAATTCTCATTCCCTCAATCTCAAAAGCAAACAAATGTATTGACTAATATTCCAATAAATCCTTTTGATGTTGATGATATTAAAAAAATATTATTAAAGGCCAAAGATGCAAAAATAAATAACAAAAAGTTGAAATTAACTCAAAACGAATTTGATGTATTAAAAAACAATGTTTTAGTTACCAAAGACGAATTTAATGACCTTGATTTAATAATTTCAAAATTAACTAAATATCATGAATATATAGACAAACTTGTAACTTCAAACTCTCAATATAAGCAAAGCGGTATAAATTCGGCAGTTGAATTAAATGATTTAAAATCTTATTTGGATGTTGCATATTATTCAGCTTCATTTAATAATATAGCGCCTGAACATGCAAAAGATTTGGAAATTTTAAAAACTATTCATTCATTAATAAAAGACAATTTAGCAAAATTAATGTTGAATAATGATCCACAAAATGTTGCTTCTAATGTCTTATCTCTATATTCATTATGA", "aa": "MEQLVKHEHYPIVANNKIYKDFNTESLTSSDYLTSFFGAISSNIDGKINSGKISQIQESVIQMFNFSNKTKRILGKLNITIPDYDNNKITLFDLINMSKFSFPQSQKQTNVLTNIPINPFDVDDIKKILLKAKDAKINNKKLKLTQNEFDVLKNNVLVTKDEFNDLDLIISKLTKYHEYIDKLVTSNSQYKQSGINSAVELNDLKSYLDVAYYSASFNNIAPEHAKDLEILKTIHSLIKDNLAKLMLNNDPQNVASNVLSLYSL", "aa_hexdigest": "f3474509d1da9e225b0e6db4600a5d85", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30079.960100000048, "isoelectric_point": 7.763774299621583 }, "id": "AHCKFKLKGB_744", "locus": "GCA013367975_00734" }, { "type": "cds", "sequence": "contig_1", "start": 429429, "stop": 430034, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTATAAGTTTCGGGGCAATAAACAACAATTTAACTAACGAAATTATTCAAAATAAAACAATAAATGGTTTAGTTAATGTTATTAGAGATGCCTTATTTAATAGTGAAATTATTAACATGTTCAATAAAGACGATTTATTGAGAATCGTGGATGCAGCAAAACAAAGTGTTGTGGATAATGATTTCCATACATTGCTAAATATTGGTAAGGTAAATGGCACGATTTTACAAAAAGTTCTTTTAGATATTTTAAAATACATTATTAGACCAGTAGATAGAACAAATAGTAACAATTTAAATATTAATAATGTTAATGTTAATGCTTTTGTTCAAAATGTATTCATTGGAATAATAAACTATTTAGAAAAAATGTATATGAATTCAGGAACCAATGACAATGAACGAAATCAATATTTAATGCAACAAGTTTACAACATAAGCAAACTATTAAATCTTAATAATCTATATTTGATTCCTTCTTTGAAATTAAGTTTATTTGACATAATTAATTTAGTTAAAAATAAATCAACAATTTTTGAAGTATTGAGAAATGTTATTCGCTCTATTGATTTTGAAAAATTTTCTTTAATTATTCAAGATTGA", "aa": "MPISFGAINNNLTNEIIQNKTINGLVNVIRDALFNSEIINMFNKDDLLRIVDAAKQSVVDNDFHTLLNIGKVNGTILQKVLLDILKYIIRPVDRTNSNNLNINNVNVNAFVQNVFIGIINYLEKMYMNSGTNDNERNQYLMQQVYNISKLLNLNNLYLIPSLKLSLFDIINLVKNKSTIFEVLRNVIRSIDFEKFSLIIQD", "aa_hexdigest": "eef1252c9edf030cfe9f0a8f6f8354e3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23078.44580000002, "isoelectric_point": 7.875409507751465 }, "id": "AHCKFKLKGB_745", "locus": "GCA013367975_00735" }, { "type": "cds", "sequence": "contig_1", "start": 430179, "stop": 430358, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAACACAAAAATACCTTATGAAATTGATGGAAAAATATTGATGTTGACTGTTGCTAATGACGAACCAAAGACCGTTGGTTCTTCAATAGTTGGTGCAGTTGCAAGAATTAGTGATGAAAAATACATTTTATTAAAATATTCAACTATTGACGGATTTAGTGATAAAAAAGTTTGA", "aa": "MPNTKIPYEIDGKILMLTVANDEPKTVGSSIVGAVARISDEKYILLKYSTIDGFSDKKV", "aa_hexdigest": "be2686358a6ebaa3cc9d5e47637ab526", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6474.4519, "isoelectric_point": 6.099187660217283 }, "id": "AHCKFKLKGB_746", "locus": "GCA013367975_00736" }, { "type": "cds", "sequence": "contig_1", "start": 431238, "stop": 431519, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACACTATTGATGGTAAAGATGTAATGAAGATGCTAGGTTTTAAAGAAATTTCAGAAAACCATTATGAATTTGATGCATCTCTTTATAATAAAAACTCTGATTTAAAAATCGAAAATTCAGATTTTACAAGTCAAAAATCAAAATTTATTAATGAAGACTTTAATATTTCAAGAACTTTTAAACTTCAAAATTTCTTAAGTAATAAAAGTATTGAAAAAGATGTTAATGAATTTACAAAACTAGCAGCAAATAAAATATATCAAATTCCAAATGAATGA", "aa": "MHTIDGKDVMKMLGFKEISENHYEFDASLYNKNSDLKIENSDFTSQKSKFINEDFNISRTFKLQNFLSNKSIEKDVNEFTKLAANKIYQIPNE", "aa_hexdigest": "cc67ce97dd05b14625ea52c2116d74da", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10920.116399999988, "isoelectric_point": 5.6785791397094725 }, "id": "AHCKFKLKGB_747", "locus": "GCA013367975_00737" }, { "type": "cds", "sequence": "contig_1", "start": 431535, "stop": 432317, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGACTTTTTAAAGAGGTTTTCAAATCATTGGCAAGAAATAAAGTTACCTTGATTTGTTTAACAATTTTAATTTTCTTAACAACATTTTTATTTACTTTGTTAAATGATGTTACTACTAGCTATTCATCTACGATTAATTCGTATGAAAAAGTTTCAAAATTGCATGATGCAACTGTTGATTTTGACGTTAACCCATCAGGTGTGATACCAAAATCAGGTTATAACCAAATAGGATCTGATAATCAATCAATTGTTGATAAACCTGTTGTTTTTGAGTCTTCTAATAATGGAAGTGATATTTCATATTCAATAGATCTACCAAAAGATCAAAGAAATTATGTAGGTTTAAAAAATACTTTTAATAATTTAAATTATGATAATGAAAACCATTTTATAAGTACTTATGATTTATTAAATTTATTATATTCTAGCAAGGAATCTATAAGTGATGTAGACGTTGAAATTTTAAAATCAAACCCTGATTTTTCTAAAATTAGGAATTTTAGTTTTTCAGGTCACGATAGGGAATTTAAACTTTATATAAAAGATAGCAAAGGAAATATAAAACCGTTAACTAAAAGATATAATTTGACAAAAAATGATGACATTACTTTTTTAAATGAAGTTAGATTAAAAAATATAGCTTCTATTGCATATGGTCCAAGAGGTTCACTAAACGATCAAAAAGTAGTTGATTATTTATACAATCCCTCTCCATTATTTCTAAATGTTAAAACTAAACAAGCATCATTTGATACTAAAGATTATGAAGCGTGA", "aa": "MRRLFKEVFKSLARNKVTLICLTILIFLTTFLFTLLNDVTTSYSSTINSYEKVSKLHDATVDFDVNPSGVIPKSGYNQIGSDNQSIVDKPVVFESSNNGSDISYSIDLPKDQRNYVGLKNTFNNLNYDNENHFISTYDLLNLLYSSKESISDVDVEILKSNPDFSKIRNFSFSGHDREFKLYIKDSKGNIKPLTKRYNLTKNDDITFLNEVRLKNIASIAYGPRGSLNDQKVVDYLYNPSPLFLNVKTKQASFDTKDYEA", "aa_hexdigest": "b937ed926af749327d1753f14cec9a31", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29692.063300000056, "isoelectric_point": 7.699773597717285 }, "id": "AHCKFKLKGB_748", "locus": "GCA013367975_00738" }, { "type": "cds", "sequence": "contig_1", "start": 432502, "stop": 432840, "strand": "-", "frame": 1, "gene": "rsmG", "product": "RsmG family class I SAM-dependent methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D54A", "UniRef:UniRef90_UPI002410D54A" ], "nt": "TTGGTTAAAAATGAGTTGAAATTGAAAAATATTGATATACAAAAAATTCGTGCTGAAGATTCAAATCAAATAGAATGTTTTGATTTTATTAGTGCAAGAGCGGTGAGTGAATTAAAAAATTTATTAGAAATTTCACACCATCTTTTAAAAATTAATGGAATTGCTTGCTTTTTAAAATCAAATAAATATCAGCAAGAAATAGCTAATTCAAACTTTATAGTTAACAAATTAAATATTAATTTAAAAACAAGAATTTTGCCAATATATTTCAATATTGAAAATGTTTTAATTTTTCACCAAAAATTAATAAAAACAGCCTCAGGATTTCCTAGAAAATGA", "aa": "MVKNELKLKNIDIQKIRAEDSNQIECFDFISARAVSELKNLLEISHHLLKINGIACFLKSNKYQQEIANSNFIVNKLNINLKTRILPIYFNIENVLIFHQKLIKTASGFPRK", "aa_hexdigest": "8dd8912789829373f67b46d2acf1ba05", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI002410D54A", "query_cov": 1.0, "subject_cov": 0.9032258064516129, "identity": 1.0, "score": 219.0, "evalue": 3.28e-72, "valid": true, "product": "RsmG family class I SAM-dependent methyltransferase", "uniref50_id": "UniRef50_UPI002410D54A" }, "pscc": { "uniref50_id": "UniRef50_UPI002410D54A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D54A" ], "product": "RsmG family class I SAM-dependent methyltransferase" }, "genes": [ "rsmG" ], "id": "AHCKFKLKGB_749", "locus": "GCA013367975_00739" }, { "type": "cds", "sequence": "contig_1", "start": 432882, "stop": 433181, "strand": "-", "frame": 2, "gene": "rsmG", "product": "RsmG family class I SAM-dependent methyltransferase", "db_xrefs": [ "RefSeq:WP_277872598.1", "SO:0001217", "UniParc:UPI00241117DC", "UniRef:UniRef100_UPI00241117DC", "UniRef:UniRef50_UPI001F51865A", "UniRef:UniRef90_UPI00241117DC" ], "nt": "ATGCTTACGGAAAAAGAAAGCAATAATTTAATATTGCAAAGTCTACCGCAATGTAGCAAAGAAGTCTTAAAAAAATTATATGAATATTATCAATTAATAGAACAAGAAAATAGCAAATATAATTTAACAGGTTTCTATAACGAAAAGCTTCTAAAAGAAGCAATAATTGAATCAATTTTAATTTTTTTAGACATTGAAAAAAATATTTGTAATCTAACTAACAAATCTGTACTCGACATTGGCTCGGGCGCAGGATTTCCTATCCTTCCATATTTCATATATAAACAAGATTTTCTTTAA", "aa": "MLTEKESNNLILQSLPQCSKEVLKKLYEYYQLIEQENSKYNLTGFYNEKLLKEAIIESILIFLDIEKNICNLTNKSVLDIGSGAGFPILPYFIYKQDFL", "aa_hexdigest": "6c725dd8a94693e3f1261d161b6fb146", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI00241117DC", "ncbi_nrp_id": "WP_277872598.1", "uniref100_id": "UniRef100_UPI00241117DC", "db_xrefs": [ "SO:0001217", "UniParc:UPI00241117DC", "RefSeq:WP_277872598.1", "UniRef:UniRef100_UPI00241117DC" ] }, "ips": { "uniref100_id": "UniRef100_UPI00241117DC", "uniref90_id": "UniRef90_UPI00241117DC" }, "psc": { "uniref90_id": "UniRef90_UPI00241117DC", "product": "RsmG family class I SAM-dependent methyltransferase", "uniref50_id": "UniRef50_UPI001F51865A" }, "pscc": { "uniref50_id": "UniRef50_UPI001F51865A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51865A" ], "product": "RsmG family class I SAM-dependent methyltransferase" }, "genes": [ "rsmG" ], "id": "AHCKFKLKGB_750", "locus": "GCA013367975_00740" }, { "type": "cds", "sequence": "contig_1", "start": 433156, "stop": 434130, "strand": "-", "frame": 1, "gene": "prsA", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "KEGG:K00948", "RefSeq:WP_012855629.1", "SO:0001217", "UniParc:UPI0001BC237C", "UniRef:UniRef100_D1J8E3", "UniRef:UniRef50_Q98R83", "UniRef:UniRef90_D1J8E3" ], "nt": "ATGAAAGCAAAAAAAGAAGATAATGTTGTAATTTTTGGCATGGAAAATTCCCAAAAATTAGCACAAGAAATTGGAAAGTATTTAAATAAAGAAATATTGCCAGTTAAAACTACAGTATTTGCAGATGGAGAATGCCTTTTAAAATCAGAAACAACCGTTAGAAATAAGGATGTTTACATTATTTGCAATACTTCAAAGCCAGTAAACGATAACATAATGTCATTGCTGATTTTAATTGATTCATTAAGACGTTCATCAGCAAAGAGCATTAATTGCGTTATGACCTACTATGGATATGCAAGACAAGATAGAAAAGCAAGTGGAAGAGATCCAATATCAGCTGCACTTGTTGCTAAGATGTATGAAACAGCGGGTGCTGATAAAATAATTACTATTGACTTGCACAACGCTTCAATTCAAGGATTTTTCAACATTCCTGTTGATGATTTAAGGGGACAATTTATTTTTTCAAATGAATTGCTAAACAGAAAAGAAAAACTAGTAATTGTATCACCAGACCACGGGGGTGCAGTTAGAGCAAGACAATTGTCAGAATTACTAAACAATGAAGAACAAATTGCAATTATTGACAAGCGTCGTACAGGACCTAATAAATGCGAAATTATGGGTGTTTTAGGTGATGTTAAAGATAAAAATGTTTTAATTTATGATGATATTATTGACACAGGTGGAACAATTATTCATGCAGCAGAAGCGTTAAAAGCAAACGGCGCAAAAAATATTATAATTGCAGCTACTCATGGTTTATTTTCAAAAGGGTTTGAAATGTTTGAAAATAACGCAATGATTGATGAAGTTATTATTACTAATTCAATAGATCACAGCCACTTATCAAACTTTAAAAAATTAAGAGAATTATCTTTGGCTCAATTTTTAAGTATGGTAATCAATGCAAACATTGAAAGCAAATCTATAACAGAAGTATATAATGCTTACGGAAAAAGAAAGCAATAA", "aa": "MKAKKEDNVVIFGMENSQKLAQEIGKYLNKEILPVKTTVFADGECLLKSETTVRNKDVYIICNTSKPVNDNIMSLLILIDSLRRSSAKSINCVMTYYGYARQDRKASGRDPISAALVAKMYETAGADKIITIDLHNASIQGFFNIPVDDLRGQFIFSNELLNRKEKLVIVSPDHGGAVRARQLSELLNNEEQIAIIDKRRTGPNKCEIMGVLGDVKDKNVLIYDDIIDTGGTIIHAAEALKANGAKNIIIAATHGLFSKGFEMFENNAMIDEVIITNSIDHSHLSNFKKLRELSLAQFLSMVINANIESKSITEVYNAYGKRKQ", "aa_hexdigest": "4ad11e3f20055d1b39cae4c28e53011b", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC237C", "ncbi_nrp_id": "WP_012855629.1", "uniref100_id": "UniRef100_D1J8E3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC237C", "RefSeq:WP_012855629.1", "UniRef:UniRef100_D1J8E3" ] }, "ips": { "uniref100_id": "UniRef100_D1J8E3", "uniref90_id": "UniRef90_D1J8E3" }, "psc": { "uniref90_id": "UniRef90_D1J8E3", "gene": "PrsA", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_Q98R83", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948" }, "pscc": { "uniref50_id": "UniRef50_Q98R83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R83" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [ "prsA" ], "id": "AHCKFKLKGB_751", "locus": "GCA013367975_00741" }, { "type": "cds", "sequence": "contig_1", "start": 434324, "stop": 434512, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACTCAAACCCTTGTAATTCACTTTTTGCGTACTGATAAAACACCATTTATTAAATCAAATGCAACGACCTCGTTATTTATTTCAACATTAATCGGATGTGCAATAATTAATGCTGTGCCATATATACCGGGAATTAATAAAGGTTTAAGTCTAATGCCATTACCAGGAATTTATTATGCTTGA", "aa": "MYTQTLVIHFLRTDKTPFIKSNATTSLFISTLIGCAIINAVPYIPGINKGLSLMPLPGIYYA", "aa_hexdigest": "5bb3a49d8d55cdb133e821f609318a28", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6804.0436, "isoelectric_point": 9.360567665100099 }, "id": "AHCKFKLKGB_752", "locus": "GCA013367975_00742" }, { "type": "cds", "sequence": "contig_1", "start": 434795, "stop": 436099, "strand": "-", "frame": 3, "gene": null, "product": "Magnesium-transporting ATPase, P-type 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X4XS16" ], "nt": "TTGTTTGGTATATCAATTGCTGTTGGTTTAACACCAGAAATGCTTCCAATGATAGTGACTGCTAGTTTATCTAAAGGCGCAATTTCACTAGGCAAGGCCAAAACAATAATTAAAAATATTAATTCAATTCAAAATTTTGGTTCAATGGACATTTTATGTACAGATAAAACCGGAACATTAACACAAGATGAAGTAATTATGGAACGTCATATGGATGTTACCGGCAAAGAAGATTTTCATGTTTTAAAATATGCTTTTATTAATTCATATTATCAAACAGGATTGAAAAATTTACTTGATAAATCAATAATTAATAAGACTTTTGAAATGGCATCAAAAAACAAAGAACTTGAAGATTTTCAAAGATTTTATGAAAAAATTGATGAAATTCCTTTTGATTTCAAAAGAAAAAGAATGTCTGTTTTAGTTAAATCATTAAAACAAAATAAAGTAACCTTGATTACAAAAGGTGCTGTTGAAGAAATTGTGAGTGTATGCAAAAATATTTACATTGATGGAGAAGTTAAGCCTCTTGATAAAAAAACAATAGATAAGGTTCTAAGAAGAGTTGAAGAATTCAATTCAGAAGGACTAAGAGTTATTGCGATAGCTTCTAAAAAATCAACAATTAATGCAGTTGGAAAAATATCAGTAAATGATGAATGCGATATGACTTTGATTGGATATTTAACATTTCTTGATCCTCCTAAAGATTCTGCTGCTGCCGCAATTGAAAAATTGCATGAATATGGAGTTGATGTAAAAATATTAACGGGAGATAGTGCGCTTGTAACAAAAGCAGTATGTGCCCAAGTAGGAATAAAAAATAATAGAATTTTACTAGGCAAAGAAGTTGAAGAATTAAGTGATGCTGAACTTAAAATTGAAGTTGAAAAAGTTCAAATTTTTGCAAAATTAAGCCCTGACCAAAAGGCAAGAATTATTTCAATATTGCGTGATAATGGTCATGTTGTTGGCTATATGGGTGACGGAATTAATGATGCACCCGCTATGAAATCAGCCGATGTTTCAATATCAGTTGATACTGCAGTTGATATTGCAAAAGAATCAGCTAACATAATTCTTTTAGAAAAAGACCTAAATGTTTTGGCTAAAGGAATTGTTGAAGGTAGAAAAACACATGCCAATATTAACAAATACATAAAAATGACTGCATCTTCAAACTTTGGAAATATATTTAGTATTTTAATTGCTTCATTTTTAATTCCTTTTGTTCCATTAAACCCTGTACAAATAATATTATTGAATTTGGTATATGATGTTTGCTGTGCAACAATGCCATGA", "aa": "MFGISIAVGLTPEMLPMIVTASLSKGAISLGKAKTIIKNINSIQNFGSMDILCTDKTGTLTQDEVIMERHMDVTGKEDFHVLKYAFINSYYQTGLKNLLDKSIINKTFEMASKNKELEDFQRFYEKIDEIPFDFKRKRMSVLVKSLKQNKVTLITKGAVEEIVSVCKNIYIDGEVKPLDKKTIDKVLRRVEEFNSEGLRVIAIASKKSTINAVGKISVNDECDMTLIGYLTFLDPPKDSAAAAIEKLHEYGVDVKILTGDSALVTKAVCAQVGIKNNRILLGKEVEELSDAELKIEVEKVQIFAKLSPDQKARIISILRDNGHVVGYMGDGINDAPAMKSADVSISVDTAVDIAKESANIILLEKDLNVLAKGIVEGRKTHANINKYIKMTASSNFGNIFSILIASFLIPFVPLNPVQIILLNLVYDVCCATMP", "aa_hexdigest": "6dbfabd812017564fb8b11baea567c9d", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0001C17514", "query_cov": 0.9976958525345622, "subject_cov": 0.9039665970772442, "identity": 0.524, "score": 444.0, "evalue": 1.63e-150, "valid": false, "gene": "mgtA", "product": "magnesium-translocating P-type ATPase", "ec_ids": [ "7.2.2.14" ], "uniref50_id": "UniRef50_A0A2X4XS16", "kegg_orthology_id": "K01531", "go_ids": [ "GO:0015444", "GO:0015693", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2X4XS16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X4XS16" ], "product": "Magnesium-transporting ATPase, P-type 1" }, "genes": [], "id": "AHCKFKLKGB_753", "locus": "GCA013367975_00743" }, { "type": "cds", "sequence": "contig_1", "start": 436112, "stop": 436672, "strand": "-", "frame": 3, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "nt": "ATGGTTTTTTTAAGTGGAATTATTCACTTCGTTCAAGAACAACGTGGGGCATCAAGCGCTGATAAATTAGTTGAGACTGTTGAAACTACTTCGCTAGTTCAAAGGAATGGCATTATGAAGGAAATTCCAATGGATGAGATTGTAGTAGGCGATATTGTGTATTTAGCAGCTGGAGATATTATTCCGGCGGATGTAAGAATATTAGAAGCAAAAGACTTATTTATTTCTCAATCCTCATTAACCGGAGAAAGCGATGCTATTGAAAAATATAAGAACATCGACAGTTCTAAAGAATTTGAAGCAATAACTGATCGTAGCAATTTAGCTTTTATGGGAACCAATGTTATTTCAGGTTCTGCTAAAACTATTGTAATTGCAACCGGCAATGATACAATTATTGGACAAGTTGCTTCCAAAATCAATGAAAAACCAATACCAACAAACTTTGATAAAGGAATTAAAAAAATTTCAATAATGTTCACTATTATAATGGCCGTTATTGTCCCTATAGTATTTTTAATTTTAGGAATTAAAGGTGTTATTGGTGGAGAAAACCAATGA", "aa": "MVFLSGIIHFVQEQRGASSADKLVETVETTSLVQRNGIMKEIPMDEIVVGDIVYLAAGDIIPADVRILEAKDLFISQSSLTGESDAIEKYKNIDSSKEFEAITDRSNLAFMGTNVISGSAKTIVIATGNDTIIGQVASKINEKPIPTNFDKGIKKISIMFTIIMAVIVPIVFLILGIKGVIGGENQ", "aa_hexdigest": "068c32b7a2457ef169dae19419f14c69", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AC587A1", "query_cov": 0.9247311827956989, "subject_cov": 0.900523560209424, "identity": 0.541, "score": 157.0, "evalue": 1.48e-45, "valid": false, "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI002AC587A1" }, "pscc": { "uniref50_id": "UniRef50_UPI002AC587A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "product": "HAD-IC family P-type ATPase" }, "genes": [], "id": "AHCKFKLKGB_754", "locus": "GCA013367975_00744" }, { "type": "cds", "sequence": "contig_1", "start": 436683, "stop": 436904, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGACAGTCATTCCATCTATTCATGTTATCTTTTTACCTTTTGATGCCGGCAATATTAAATCAATAATCATAGAAACAATTGCCAATGCAAACAAAATGATGTTAAAAAAATTAAAAAATGATCTAAATAAACGCTTTCAAACATTATCAGCGCCTTTTTTTGTTATTTTATTTAAACCATATTCTTCTCTTGCAACTTCAATTCTATCTTCGTCTTGA", "aa": "MMTVIPSIHVIFLPFDAGNIKSIIIETIANANKMMLKKLKNDLNKRFQTLSAPFFVILFKPYSSLATSILSSS", "aa_hexdigest": "67f197011df8f7d7f53af322caf7baf3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8160.720099999997, "isoelectric_point": 9.998095512390137 }, "id": "AHCKFKLKGB_755", "locus": "GCA013367975_00745" }, { "type": "cds", "sequence": "contig_1", "start": 436823, "stop": 437050, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGTTAATATAACAAAAAATAAACTTAAAAATTCTTCAAGAAAAGAAAAACAAATTAAGCAAATCAATACAAGGTTAGTTGAAGCTTCAAATTGTAGTCAAGAAGAATTATTTTTAAAATATCAAACTTCTCAAAATGGATTTCAAGACGAAGATAGAATTGAAGTTGCAAGAGAAGAATATGGTTTAAATAAAATAACAAAAAAAGGCGCTGATAATGTTTGA", "aa": "MIVNITKNKLKNSSRKEKQIKQINTRLVEASNCSQEELFLKYQTSQNGFQDEDRIEVAREEYGLNKITKKGADNV", "aa_hexdigest": "5a65f7974afac9ca319e1ab4e9043a1f", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8704.731499999994, "isoelectric_point": 9.130415534973146 }, "id": "AHCKFKLKGB_756", "locus": "GCA013367975_00746" }, { "type": "cds", "sequence": "contig_1", "start": 437241, "stop": 437516, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGGTTGATACTTATTTTATTACAAAAAGTTGACATCATTTTAGCAGACGAACCTACTAATAGTCTTGATATAGCAAACGCAAGAGATGTTTTAGAATTATTGTCTTCAATATCACAAGAATACAAAAAAACAATTTTAGTAAATATTCACGACATTTCATTAATAAAAACTTATTTTAAGAAATACATTTATGTCAAAGATGGACAAATTATTGAAATTGGCGAAACAAAAAATTTAAATGAATTAAAAATAAAAGATTTATATAATTTTTAA", "aa": "MWLILILLQKVDIILADEPTNSLDIANARDVLELLSSISQEYKKTILVNIHDISLIKTYFKKYIYVKDGQIIEIGETKNLNELKIKDLYNF", "aa_hexdigest": "477e37b7292077b0aedcc44a98fda922", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10612.329799999989, "isoelectric_point": 5.278318977355956 }, "id": "AHCKFKLKGB_757", "locus": "GCA013367975_00747" }, { "type": "cds", "sequence": "contig_1", "start": 437754, "stop": 437984, "strand": "-", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AFED49B" ], "nt": "ATGAAAAAAATTGAATTTATCAATGTTGATGCAAGTTATTATAAAAAAAGTCCAATAGTTTTAAAGAACATAAATTTTTCTATTGAAGAGGGTGAATTTATAGCGATAATAGGAAAATCCGGAGCTGGAAAATCAACAATTTTCAATGCTTTGTTGAGGCAATTATTTGTAAAAAAAGGCAATGTAAAAATTAATGATATTGATATTTATAGTGCTTCAAAAAAACAATGA", "aa": "MKKIEFINVDASYYKKSPIVLKNINFSIEEGEFIAIIGKSGAGKSTIFNALLRQLFVKKGNVKINDIDIYSASKKQ", "aa_hexdigest": "597d614b91455d10362c1195bef78c85", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AFED49B", "query_cov": 0.8552631578947368, "subject_cov": 0.8783783783783784, "identity": 0.585, "score": 79.3, "evalue": 5.23e-18, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI002AFED49B" }, "pscc": { "uniref50_id": "UniRef50_UPI002AFED49B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AFED49B" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_758", "locus": "GCA013367975_00748" }, { "type": "cds", "sequence": "contig_1", "start": 438099, "stop": 438425, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02866.23" ], "nt": "ATGGTTAAAAAAGAAGCATTTGAAATTATTGCCGGTAAAGGCATTACCAATTATGGTATTGGCGAAAACCTTGCTGAAATTACACAAGCAATTTTGTATGATAGACAAAGAATTTATTCTCTTGGAGTTCAATTGCCAAAGGAATATAAACATGCCGGAGTTTACTTTGGTTTGCCAGTAATCTTGGGTAAGAATGGTTATACACACTTGCCAAAAATTAGACTTGACGAAGATGAACAACAACAATTTGATGAATATTCAAAAGAAATTAAAGAAACAGCAATTCAAGTATTAAAAAATTTGGATGTTGAAATTACTATTAAATAA", "aa": "MVKKEAFEIIAGKGITNYGIGENLAEITQAILYDRQRIYSLGVQLPKEYKHAGVYFGLPVILGKNGYTHLPKIRLDEDEQQQFDEYSKEIKETAIQVLKNLDVEITIK", "aa_hexdigest": "0ff069a0685e97f7c251b507af1b4ee9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02866.23", "name": "Ldh_1_C", "length": 97, "aa_cov": 0.8611111111111112, "hmm_cov": 1.0, "evalue": 3.447259136409734e-15, "score": 51.76319122314453, "start": 2, "stop": 94 } ], "seq_stats": { "molecular_weight": 12343.04149999999, "isoelectric_point": 5.687104988098144 }, "id": "AHCKFKLKGB_759", "locus": "GCA013367975_00749" }, { "type": "cds", "sequence": "contig_1", "start": 438474, "stop": 439067, "strand": "-", "frame": 2, "gene": "mdh", "product": "L-lactate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0039", "SO:0001217", "UniRef:UniRef50_A0A2Z4LNL4", "UniRef:UniRef90_D1J8E6" ], "nt": "ATGACTAAAATAGGATTGATTGGCGTTGGTGCTGTTGGTAGTTCATATTTATATGCTGCATTAAATAAAAATATAGAAGCAGATTATGTTTTAATTGATAGCTTTCCTCAAGTTGCAATTGCACAAGCAAAGGATTTAAATGATGCTGCTTGTTCAATGGCAAATAATGGTTCAACATTCAAGGCTGGAGATTATCCAGATTTGAAAGATGCTGATATTGTCATTATTACTGCATCAATTAGACCAAAGGAAGGCGTTTTAAAAGATAGAATGGAATTATTGACCGATAATGCAAAACTATTGAGAAAAATCGGTCAAAGCATAAAAGATTCAGGATTTGCTGGTGTTACAATCATTGCTTCAAACCCTGTTGACATTATGGCAACAATTTATCAACAAGTAACCAAGTTTTCTCCAAATAAAGTTATTAGTTCAGGAACTATTCTAGAAACTGCCAGAATGAAAAAATTTCTTTCAGAAAAAATTGGAATTAAAGCACACTCAATTACTGGATTTGTTATTGGCGAACATGGAGCAAGATGCTTAATCCCATTTTCAAAAATAAGAATTGGTTTGGGTTCATTAAAAGATTGA", "aa": "MTKIGLIGVGAVGSSYLYAALNKNIEADYVLIDSFPQVAIAQAKDLNDAACSMANNGSTFKAGDYPDLKDADIVIITASIRPKEGVLKDRMELLTDNAKLLRKIGQSIKDSGFAGVTIIASNPVDIMATIYQQVTKFSPNKVISSGTILETARMKKFLSEKIGIKAHSITGFVIGEHGARCLIPFSKIRIGLGSLKD", "aa_hexdigest": "8bd6037b667dd6d64f4223a898a51693", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "mdh" ], "truncated": "5-prime", "pseudogene": { "start": 438171, "stop": 439067, "observations": { "mutation": [ 438446, 438476 ], "stop": [ 438446, 438476 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E6", "query_cov": 1.0, "subject_cov": 0.6118012422360248, "identity": 0.99, "score": 376.0, "evalue": 9.18e-130, "gene_start": 1, "gene_end": 197, "reference_start": 1, "reference_end": 197, "reference_sequence": "MTKIGLIGVGAVGSSYLYAALNKNIEADYVLIDSFPQVAIAQAKDLNDAACSMANNGSTFKAGDYSDLKDADIVIITASIRPKEGVLKDRMELLTDNAKLLRKIGQSIKDSGFDGVTIIASNPVDIMATIYQQVTKFSPNKVISSGTILETARMKKFLSEKIGIKAHSITGFVIGEHGARCLIPFSKIRIGLGSLKDWLANGTITNEWLSQLNEMVKKEAFEIIAGKGITNYGIGENLAEITQAILYDRQRIYSLGVQLPKEYKHAGVYFGLPVILGKNGYTHLPKIRLDEDEQQQFDEYSKEIKETAIQVLKNLDVEITIK" }, "paralog": false, "identity": 0.9865771812080537, "score": 569.0, "evalue": 2.52e-208, "description": "Internal stop codon at 438446, 438476. Nonsense mutation around 438446, 438476.", "psc": { "uniref90_id": "UniRef90_D1J8E6", "gene": "Mdh", "product": "L-lactate dehydrogenase", "uniref50_id": "UniRef50_A0A2Z4LNL4", "cog_id": "COG0039", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E6", "UniRef:UniRef50_A0A2Z4LNL4", "COG:COG0039", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z4LNL4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z4LNL4" ], "product": "L-lactate dehydrogenase" } }, "id": "AHCKFKLKGB_760", "locus": "GCA013367975_00750" }, { "type": "cds", "sequence": "contig_1", "start": 439204, "stop": 439539, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTTAGTGAAACTATTTCATCTATAAATAACAGGATTTATATTGAAACATGCTTTGGAGGAGTTGATAATAAAACTTTGGCTTCAAATTACACCAATACTCCATTTAAGTGTTTTGATACTTGTTTGAAATTTCAAGAACCTATTATGGACTGCAAAATTTCACAAAATAATGAAAATGAAACAGATGGTTTGAATTATTTAAGCAATTTAACTTATAATAATTTATGTAATCTTGTAATGGAAACATTTATTGAAAACCATGTTAACGTATATAAAATACCTAACATTCAAATCATGATTGACAATTATAATGATAGTACATTGGGTTGA", "aa": "MLFSETISSINNRIYIETCFGGVDNKTLASNYTNTPFKCFDTCLKFQEPIMDCKISQNNENETDGLNYLSNLTYNNLCNLVMETFIENHVNVYKIPNIQIMIDNYNDSTLG", "aa_hexdigest": "50a46ab84c98ab08bfef6426eafd2a35", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12758.223899999986, "isoelectric_point": 4.352127647399903 }, "id": "AHCKFKLKGB_761", "locus": "GCA013367975_00751" }, { "type": "cds", "sequence": "contig_1", "start": 439543, "stop": 440379, "strand": "-", "frame": 1, "gene": "pgi", "product": "Glucose-6-phosphate isomerase (GPI)", "db_xrefs": [ "COG:COG0166", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J8E7", "UniRef:UniRef90_D1J8E7" ], "nt": "ATGGAAAATAAGCAAATTATTGACATAAATTTAAAAAAAGCAATTAAAGAAAGTGCACCAAACCGTTATTATGAGGCGGTTCAAAAAATCTTGTTAGATATAAAAAACTCAAAGGTTAAAAATTCAAATTATTTAGGTGTATTCAATGTTTTTAAAAATGTAAAGAAATCTGATTATGAAAAAATTCAAGAAATATCAAGCGAATTATTATCAGATGGTATTGAATATTTAGTAATAATAGCGACTCAAACAATAATTAATCAAATTAAGGCACTTGCTGATTTGCATTTTGATTTAAGAAAAGAAAATGAATCAATTAAATTGATTTATGTTAATGAAAATTTTAATAGTTTAGAAGTTGCTAAATTGCTTATTAAACTTCAAAATAAAAAGTTTGCTATTAATGTAATCTCACAAACTGGCGAATGCTTAGAAACCTTAATAATGTTTAGAGAATTAAAAAACTTATTATATAAAGAGCTAGGCAAGGCAGGTGCTTCAAAATATATTTATGTAACAACAAACAATAATTATGGAAGATTATTTAATGATGCCAAGATAAATCAATATACTCATTTAACAATTTTAGATAATACCGTCGAAAGATTTTTAAGTTATTCACCTGCAATTTTATTACCAATTGCTTGTATAGGAATTAATATTGAAAAATATTTATCTGGTGCTCATAAGGCTAATGAATTTTTTTTAAATGCACCATTAGAAAAAAACCCAGCAGTTTTATATGCAATCACTAGAAAAATCCTTCATGATAACAATTTTATATTTGAAAATATTTGCTATTTCGAGCAAAGTCAAGAATCTTTAGCAAAATTGTGA", "aa": "MENKQIIDINLKKAIKESAPNRYYEAVQKILLDIKNSKVKNSNYLGVFNVFKNVKKSDYEKIQEISSELLSDGIEYLVIIATQTIINQIKALADLHFDLRKENESIKLIYVNENFNSLEVAKLLIKLQNKKFAINVISQTGECLETLIMFRELKNLLYKELGKAGASKYIYVTTNNNYGRLFNDAKINQYTHLTILDNTVERFLSYSPAILLPIACIGINIEKYLSGAHKANEFFLNAPLEKNPAVLYAITRKILHDNNFIFENICYFEQSQESLAKL", "aa_hexdigest": "c810b37d96da64801b5a32295d339b10", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "pgi" ], "truncated": "5-prime", "pseudogene": { "start": 439240, "stop": 440379, "observations": { "mutation": [ 439545 ], "stop": [ 439545 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E7", "query_cov": 1.0, "subject_cov": 0.6303854875283447, "identity": 0.975, "score": 526.0, "evalue": 1.02e-185, "gene_start": 1, "gene_end": 278, "reference_start": 1, "reference_end": 278, "reference_sequence": "MENKQIIDINLKKAIKESAPSRYYETVQKILLDIKNSKVKNSNYLGVFNVFKNVKKSDYEKIQEISSELLSNGIEYLVIIATQTIINQIKALADLHFDLRKENESIKLVYVNENFNSLEVAKLLIKLQNKKFAINVISQTGECLETLIMFRELKNLLYKELGKAGASKYIYVTTNNNYGRLFNDAKINQYTHLTILDNTVERFLSYSPAILLPIACIGINIEKYLSGAHKANEFFLNAPLEKNSAVLYAITRKILHDNNFIFENVCYFEQNQESLAKLWQMLFSETISSMNNRIYIETCLGGVDNKTLASNYTNTPFKCFDTCLKFQEPIMDCKISQNNENETDGLNYLSNLTYNNLCNLVMETFIENHVNVYKIPNIQIMIDNYNDSTLGWIMSFLHHSSILFAYLNEQNPFERKPLESYNFNLAKNFKELLGGKKKWLK" }, "paralog": false, "identity": 0.9736147757255936, "score": 736.0, "evalue": 4.13e-271, "description": "Internal stop codon at 439545. Nonsense mutation around 439545.", "psc": { "uniref90_id": "UniRef90_D1J8E7", "gene": "Pgi", "product": "Glucose-6-phosphate isomerase (GPI)", "uniref50_id": "UniRef50_D1J8E7", "cog_id": "COG0166", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E7", "UniRef:UniRef50_D1J8E7", "COG:COG0166", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8E7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8E7" ], "product": "Glucose-6-phosphate isomerase (GPI)" } }, "id": "AHCKFKLKGB_762", "locus": "GCA013367975_00752" }, { "type": "cds", "sequence": "contig_1", "start": 440369, "stop": 440608, "strand": "-", "frame": 3, "gene": "yabR", "product": "30S ribosomal protein S1", "db_xrefs": [ "COG:COG1098", "COG:R", "SO:0001217", "UniRef:UniRef50_A0A3T0TTP6", "UniRef:UniRef90_D1J8E8" ], "nt": "TTGAGTATAAATGAAGTTAGTGACTATTACATTAGCGATTTAAAATTAATGTTTAAAATCAATGATATTAAGGAAGTTCAAGTAATTGAAATAATGCCAAATAATGATTTAGTAGTTTCATTTAAAAGAATTCACCCCAAGGAATTAAGAAACCCTTTCGAATTTAAACTAGATAGTCAAGATGCAGAATTTGATGGATTATTAGAATTTACTAACAAAGGAATTAATTATGGAAAATAA", "aa": "MSINEVSDYYISDLKLMFKINDIKEVQVIEIMPNNDLVVSFKRIHPKELRNPFEFKLDSQDAEFDGLLEFTNKGINYGK", "aa_hexdigest": "e2ea816ef770b351b97bd2726dfdb568", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "yabR" ], "truncated": "3-prime", "pseudogene": { "start": 440369, "stop": 440704, "observations": { "mutation": [ 440656 ], "stop": [ 440656 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E8", "query_cov": 1.0, "subject_cov": 0.7117117117117117, "identity": 0.987, "score": 159.0, "evalue": 2.4e-49, "gene_start": 1, "gene_end": 79, "reference_start": 33, "reference_end": 111, "reference_sequence": "MNYEIGDIFKAKIIKVWKNNIILQTKEGCKCFLSINEVSDYYISDLKLMFKINDIKEVQVIEIMPNNDLVVSFKRIHPKELRNPFEFKLDSQDAEFDGLLEFTNKGINYGK" }, "paralog": false, "identity": 0.990990990990991, "score": 222.0, "evalue": 1.33e-76, "description": "Internal stop codon at 440656. Nonsense mutation around 440656.", "psc": { "uniref90_id": "UniRef90_D1J8E8", "gene": "YabR", "product": "30S ribosomal protein S1", "uniref50_id": "UniRef50_A0A3T0TTP6", "cog_id": "COG1098", "cog_category": "R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E8", "UniRef:UniRef50_A0A3T0TTP6", "COG:COG1098", "COG:R" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3T0TTP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3T0TTP6" ], "product": "S1 motif domain-containing protein" } }, "id": "AHCKFKLKGB_763", "locus": "GCA013367975_00753" }, { "type": "cds", "sequence": "contig_1", "start": 441191, "stop": 441388, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTTATAACGTCAACTTTATTAAAAAATTCATTGCTAAAAAATGATGTTATTCCTTTACTTCAAACAAAAACAAGGGCTTTTTATTTTGATAAATTAGATGAAAAATATGTTGATGGTTCTGGCAATGATCCGCTAAGAAAAATAGCAAAAATAGCAAATGATTTATTTAACAAAAAGCCTTATAAATCATGA", "aa": "MAFITSTLLKNSLLKNDVIPLLQTKTRAFYFDKLDEKYVDGSGNDPLRKIAKIANDLFNKKPYKS", "aa_hexdigest": "8b3d111ea7160a229669bec8aa33c6b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7451.5965000000015, "isoelectric_point": 9.646807289123537 }, "id": "AHCKFKLKGB_764", "locus": "GCA013367975_00754" }, { "type": "ncRNA-region", "class": [ "riboswitch", "SO:0000035" ], "sequence": "contig_1", "start": 442076, "stop": 442163, "strand": "+", "label": "TPP", "product": "TPP riboswitch aptamer (THI element)", "score": 57.5, "evalue": 3.6e-07, "db_xrefs": [ "RFAM:RF00059", "SO:0000035" ], "nt": "TATAAAGAACAGGGGTGCCTTTGGCTGAGAAATACCCTCATTAGCTGATCTAGTTAATACTAGCGTAGTGAGTGTCTTAGTTTTTTGC", "id": "AHCKFKLKGB_42" }, { "type": "cds", "sequence": "contig_1", "start": 442188, "stop": 442337, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAATTTAAAAAATATTTGCTTATAGGGGCAGCGGTATTGCCTGCAATGGTTGGCGCATCAGTTTTATCTTCATCATGCGTTGATAAAATTTCAAACAATACTAAAACTTACGAAAATTTAAAATTTTCAATTAACAAGCCTTGA", "aa": "MLKFKKYLLIGAAVLPAMVGASVLSSSCVDKISNNTKTYENLKFSINKP", "aa_hexdigest": "600996eede73b2d80f6ca4e7b8329204", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5334.320699999999, "isoelectric_point": 9.732872581481931 }, "id": "AHCKFKLKGB_765", "locus": "GCA013367975_00755" }, { "type": "cds", "sequence": "contig_1", "start": 442509, "stop": 442688, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTACAATCTAGAAAAAGATAAATCAAATATTCATCCATTTTTGCAAACAATGACAAGAGCGTTTGTTTTTGATCAAGATTTTAACGCTCAATATTCAAATGGTCTAGATGATGATCCACTAATAAAAATAGCTAAATCAGCATTTGATTTATTTAACAAAAAACCTTTTGAAAATTGA", "aa": "MYNLEKDKSNIHPFLQTMTRAFVFDQDFNAQYSNGLDDDPLIKIAKSAFDLFNKKPFEN", "aa_hexdigest": "714398cd00552b056449b42ca5225220", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6932.712100000001, "isoelectric_point": 5.030216789245605 }, "id": "AHCKFKLKGB_766", "locus": "GCA013367975_00756" }, { "type": "cds", "sequence": "contig_1", "start": 442866, "stop": 443240, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACGGAAGTAGTTCTAGTGGTTCGAAATACTTGCTTCAAGAAGCATTATTTAAAAAACATTTTAATTTAAAAGGAAATGAATTTAAATCATTTTTAGAAGATAAACAAGGAAACGAAGCAAAATATATTAAAGGCAAGATTAAAAATATTGGAAAGGGTTCGTTTTCTAAATTCCATATTGTTTTTGATGACCTTGGATCTTTTGCATATACTCACAGCAAAGGTAGCACAATCTATAAATTAGATGACAAAAATTCAAAAATTGAATTTTTAACAGTTACTGAACCTTTAAAATATAACATTTTTGCTACATCATCAAATATTCCAAAAGAATTAAGAGAAATAATTGCAAATGCAATGGTTGAAGTTTGA", "aa": "MHGSSSSGSKYLLQEALFKKHFNLKGNEFKSFLEDKQGNEAKYIKGKIKNIGKGSFSKFHIVFDDLGSFAYTHSKGSTIYKLDDKNSKIEFLTVTEPLKYNIFATSSNIPKELREIIANAMVEV", "aa_hexdigest": "10e42b26bc33604673c2cd06e5e9b648", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14004.834999999981, "isoelectric_point": 9.32388515472412 }, "id": "AHCKFKLKGB_767", "locus": "GCA013367975_00757" }, { "type": "cds", "sequence": "contig_1", "start": 444077, "stop": 444388, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCTATCCAAATTCAATTAATGTCAATTACTTTCATGAACTTCTAATTCCACTACTAATATTGGCAACATTTTTAATTTTTATTGAATTTTGTAACTTTATAATATCAAATTTATTGTTTTATTCAAGAACTCAAAAAGTAAATATAAAAAAATATGCCAAAAATTCTACTATTAAAAAAATAATAATTTTTATAGCAATTGCAATAGGATTAATAATAACTATCTTTGCATGTTTATCATTAAGGGGAGAAAAATTTTATTCAACCCAAGGAAATTACTATTTCAAAGAATTATTTAATGGTGAATGA", "aa": "MIYPNSINVNYFHELLIPLLILATFLIFIEFCNFIISNLLFYSRTQKVNIKKYAKNSTIKKIIIFIAIAIGLIITIFACLSLRGEKFYSTQGNYYFKELFNGE", "aa_hexdigest": "287df4b89f3c78c1228a91d746767a75", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12032.218199999985, "isoelectric_point": 9.329364967346194 }, "id": "AHCKFKLKGB_768", "locus": "GCA013367975_00758" }, { "type": "cds", "sequence": "contig_1", "start": 444358, "stop": 444627, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACAAATTAGAGATTAATAAAAATAATGAAATTACAGGAATTGATCTTAAAATAGTGTTTCAAATTTTTTCAATTCAATATGAGCGTTTGAATAATTGTCTTGAAAGCAATAGCAGTTTAAAATACGATATAAAATATGTTAATAAAATGCTTAAAAATACCAATGCCCCAAGTTGCAAAAGCATATATAGAAGTCCAAAATTACTGTTTGTTTTTCATCCAAGAAAAACATTTTTTCATTCACCATTAAATAATTCTTTGAAATAG", "aa": "MNKLEINKNNEITGIDLKIVFQIFSIQYERLNNCLESNSSLKYDIKYVNKMLKNTNAPSCKSIYRSPKLLFVFHPRKTFFHSPLNNSLK", "aa_hexdigest": "e45194b05146868e29324e46fa03356f", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10485.107299999989, "isoelectric_point": 9.736934089660643 }, "id": "AHCKFKLKGB_769", "locus": "GCA013367975_00759" }, { "type": "cds", "sequence": "contig_1", "start": 444785, "stop": 444946, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAACAATTAAGCTAGATTTAATTACATTTATGACTTTTGAAATTGAAAAAATAACTAGAAACTTTATTACATACGGTTTATTTTCTGCTTCATTATTAGGATCTGAAACTAATCTAGGAAGATATAAAGAAATCTCCGATATTGCACCATATTTATGA", "aa": "MPTIKLDLITFMTFEIEKITRNFITYGLFSASLLGSETNLGRYKEISDIAPYL", "aa_hexdigest": "b984ef0b3c7726f9b9472d7b402879f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6077.0255, "isoelectric_point": 5.052213478088378 }, "id": "AHCKFKLKGB_770", "locus": "GCA013367975_00760" }, { "type": "tRNA", "sequence": "contig_1", "start": 445191, "stop": 445264, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 64.3, "nt": "AGGGGTTTAGTATAACGGCAGTACCGCAGTCTCCAAAACTGCTTGTGAGGGTTCGATTCCTTTAACCCCTGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 445223, 445225 ], "id": "AHCKFKLKGB_22", "locus": "GCA013367975_00761" }, { "type": "cds", "sequence": "contig_1", "start": 445408, "stop": 446028, "strand": "-", "frame": 1, "gene": "metK", "product": "methionine adenosyltransferase", "db_xrefs": [ "COG:COG0192", "COG:H", "EC:2.5.1.6", "GO:0004478", "GO:0005524", "GO:0006556", "KEGG:K00789", "SO:0001217", "UniRef:UniRef50_Q4A5F4", "UniRef:UniRef90_D1J8F2" ], "nt": "ATGAAAAGCCAAGTGACATTGGAATATGATGATGAAAGCAATAAAATAACTGTTAAAAAAGTTTTAATGTCAATTCAACATGCTGAAAATGCAAATTTAAATGAATTTAAGGATTTTATAAAGAAAAATATTGTTGATTATGTGTTAAAAGAATTTAATTTCAATTTAGATTATGATTTATTGATTAATCCAACTGGCAGATTTGTAATTGGTGGCCCTGTTGGAGATACTGGACTTACAGGTAGAAAAATAATTGTTGATACATATGGTGGTTTTGCCCACCATGGCGGAGGAGCATTTTCAGGGAAAGATGCTACAAAAATAGATAGGTCTGCTGCTTATATGGCAAGGTATATTGCTAAAAACTTAGTTGCTGCTAAGATAGCAGATAAGATTGAAATTCAATTGTCTTATGCAATAGGCTTGCCACGTCCTCAATCTATTTATATAAATTCATTTAATTCATCACAATATAATGATGAACAAATTGCTGATATTATTGAACATGTATTTGATTTAAGCGTTGAACAAATTATAAAAAAATTAGATTTATTAAAGCCAATATATCTACAAACTGCAACATACGGACATTTTGGTCGAAATGACCTTAATTTAAGTTGA", "aa": "MKSQVTLEYDDESNKITVKKVLMSIQHAENANLNEFKDFIKKNIVDYVLKEFNFNLDYDLLINPTGRFVIGGPVGDTGLTGRKIIVDTYGGFAHHGGGAFSGKDATKIDRSAAYMARYIAKNLVAAKIADKIEIQLSYAIGLPRPQSIYINSFNSSQYNDEQIADIIEHVFDLSVEQIIKKLDLLKPIYLQTATYGHFGRNDLNLS", "aa_hexdigest": "c00c48577602a988da4a892f91dfb282", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A401FK43", "query_cov": 0.9757281553398058, "subject_cov": 0.9220183486238532, "identity": 0.5670000000000001, "score": 233.0, "evalue": 1.42e-74, "valid": false, "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_A0A538A148", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0004478", "GO:0005524", "GO:0006556" ] }, "genes": [ "metK" ], "truncated": "both", "pseudogene": { "start": 445348, "stop": 446328, "observations": { "mutation": [ 445410, 446112, 446292 ], "stop": [ 445410, 446112, 446292 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8F2", "query_cov": 1.0, "subject_cov": 0.5392670157068062, "identity": 0.995, "score": 404.0, "evalue": 1.16e-139, "gene_start": 1, "gene_end": 206, "reference_start": 157, "reference_end": 362, "reference_sequence": "MKKLLTSESVGAGHPDKICDQISDAILDSLLTNDPYARVACDVLANNNIIYIGGQITTKAYSDTIKEAWKVLKPLGYDENDFNIINGIAPQSADIALGVNKEEDHELGAGDQGIIFGYATNENEFYMPWPIVLAHELLKRAEFLRRQNQFPYAKSDMKSQVTLEYDDESNKITVKKVLMSIQHAENANLKEFKDFIKKNIVDYVLKEFNFNLDYDLLINPTGRFVIGGPVGDTGLTGRKIIVDTYGGFAHHGGGAFSGKDATKIDRSAAYMARYIAKNLVAAKIADKIEIQLSYAIGLPRPQSIYINSFNSSQYNDEQIADIIEHVFDLSVEQIIKKLDLLKPIYLQTATYGHFGRNDLNLSWEKLDSVDQIKEYVAKKYQN" }, "paralog": false, "identity": 0.9846625766871165, "score": 634.0, "evalue": 6.11e-233, "description": "Internal stop codon at 445410, 446112, 446292. Nonsense mutation around 445410, 446112, 446292.", "psc": { "uniref90_id": "UniRef90_D1J8F2", "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_Q4A5F4", "cog_id": "COG0192", "cog_category": "H", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0004478", "GO:0005524", "GO:0006556" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8F2", "EC:2.5.1.6", "UniRef:UniRef50_Q4A5F4", "COG:COG0192", "COG:H", "KEGG:K00789", "GO:0004478", "GO:0005524", "GO:0006556" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5F4" ], "product": "S-adenosylmethionine synthase" } }, "id": "AHCKFKLKGB_771", "locus": "GCA013367975_00762" }, { "type": "cds", "sequence": "contig_1", "start": 446290, "stop": 446496, "strand": "-", "frame": 1, "gene": null, "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387A9BEE" ], "nt": "ATGAAAAAATTATTAACATCAGAAAGTGTAGGGGCAGGTCACCCTGATAAAATATGTGACCAAATTAGTGATGCGATATTAGATAGTTTGTTGACAAATGATCCATATGCAAGAGTTGCTTGTGATGTTTTGGCAAACAACAACATCATTTATATAGGCGGTCAAATAACTACAAAGGCATATTCTGACACAATTAAAGAAGCATGA", "aa": "MKKLLTSESVGAGHPDKICDQISDAILDSLLTNDPYARVACDVLANNNIIYIGGQITTKAYSDTIKEA", "aa_hexdigest": "8e03e4d2757fc48613e17f520dcae434", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00387A9BEE", "query_cov": 1.0, "subject_cov": 0.9444444444444444, "identity": 0.5589999999999999, "score": 81.3, "evalue": 6.23e-19, "valid": false, "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_UPI00387A9BEE", "kegg_orthology_id": "K00789" }, "pscc": { "uniref50_id": "UniRef50_UPI00387A9BEE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387A9BEE" ], "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_772", "locus": "GCA013367975_00763" }, { "type": "cds", "sequence": "contig_1", "start": 446741, "stop": 447004, "strand": "-", "frame": 3, "gene": null, "product": "Aspartate--ammonia ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ9" ], "nt": "ATGTTGAATGGGGATTTAATTGTTTATGATTCAGTAAATAAAAAAGCATTGGAAATTAGCAGTATGGGAATTAGAGTTGATAAAGATTCTTTAATTAAACAATCAAATAAAACCGAAGAAGAAATTAAAAAATTGAGTCCATATCATTGTGATTTAATAGAAGGCAAATTGCCATATACGATTGGTGGTGGTCTAGGCCAAAGTAGAATTGCAATGTTTCTATTAGAAAAACATCATATTGGTGAAGTTCAAGCATCTGCATGA", "aa": "MLNGDLIVYDSVNKKALEISSMGIRVDKDSLIKQSNKTEEEIKKLSPYHCDLIEGKLPYTIGGGLGQSRIAMFLLEKHHIGEVQASA", "aa_hexdigest": "9c327a574bde271a5fce32e86d2e8ad5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZXQ9", "query_cov": 0.9655172413793104, "subject_cov": 0.8, "identity": 0.655, "score": 117.0, "evalue": 1.62e-32, "valid": false, "product": "Aspartate--ammonia ligase", "uniref50_id": "UniRef50_A0A448ZXQ9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ9" ], "product": "Aspartate--ammonia ligase" }, "genes": [], "id": "AHCKFKLKGB_773", "locus": "GCA013367975_00764" }, { "type": "cds", "sequence": "contig_1", "start": 447005, "stop": 447199, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "TTGAGTCAAAAGTTAGCTAAAAATATTTATTTTATTAGTTCTAGCCATTTGTATAATCTTTATCCAAACCTAAGTCCTGAAGAACGCGAAGAGGTAATTTGCAAAGAATATGGTTCTGTTTTTATTTATCAAATAGGATACAATCTACCTGATGGAATTCCACATTCAAAAAGAGCATTCGACTATGATGATTGA", "aa": "MSQKLAKNIYFISSSHLYNLYPNLSPEEREEVICKEYGSVFIYQIGYNLPDGIPHSKRAFDYDD", "aa_hexdigest": "c32ba03157b52bebd1cc0eb488d55135", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 63, "aa_cov": 0.984375, "hmm_cov": 1.0, "evalue": 6.131901727501372e-23, "score": 77.0584716796875, "start": 2, "stop": 64 } ], "seq_stats": { "molecular_weight": 7508.322800000001, "isoelectric_point": 5.167596626281735 }, "id": "AHCKFKLKGB_774", "locus": "GCA013367975_00765" }, { "type": "cds", "sequence": "contig_1", "start": 447440, "stop": 447670, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "ATGCCATCATCAAAATTATCATTGAAAGATACCCAATTTGCTATTGAAAAACTAAAAGATTTGTTTTCACATCAACTTCATAGAACATTGAATTTGGTCAGGGTTTCAGCGCCATTATTTGTAAAAACCAAGAGTGGTTTAAATGACGGTTTAAATGGCGAAAAACCAGTTGTATTTTTTGCAGACGAAGTAGAAGATGAATTGCAAATTATTCATTCAATTGCCAAATGA", "aa": "MPSSKLSLKDTQFAIEKLKDLFSHQLHRTLNLVRVSAPLFVKTKSGLNDGLNGEKPVVFFADEVEDELQIIHSIAK", "aa_hexdigest": "317be684de9a1cfa53705a30ea99353e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 67, "aa_cov": 0.8552631578947368, "hmm_cov": 1.0, "evalue": 2.187647561373932e-19, "score": 65.4371566772461, "start": 12, "stop": 76 } ], "seq_stats": { "molecular_weight": 8533.764299999999, "isoelectric_point": 6.824282646179199 }, "id": "AHCKFKLKGB_775", "locus": "GCA013367975_00766" }, { "type": "cds", "sequence": "contig_1", "start": 447804, "stop": 448259, "strand": "-", "frame": 2, "gene": null, "product": "DUF885 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "nt": "ATGGATGCTGTAAAATTGGCTAACTTGTTGCAATACCAAGGTGCATTAAACGAAGCTCAATTGCGTAACATGCGTTTAGCATTAGACACTGCTTACCATGGTAAAGGTGTAAAAGGACATGAAGACTTGCCAGCAGGTGCTTCAATTAACCAAGTAAGAGAATTTATGAAGAAGAATTCATCACTAGGAATTGGTGACATAACATCAGAATCAAGACGTTACCTATCATATGTTGGTCAAGCAACCTCATATAACTCAGGTAAGGCAGTTATGATGGATCTATATACAAAAGTTCAAAAGAAACTAGGACTATCAAGAAGAGAATTTGTTGAAAAAGATAATCATAAATATGTAAAAGAATTCTTTGATGCTTTATTACGTAATAGTGCATTGCCAATGGATGCTTTAATTAAATCAGTAAGTGCTAAATACGGCCTAACTGTTGAAAAGAAATAG", "aa": "MDAVKLANLLQYQGALNEAQLRNMRLALDTAYHGKGVKGHEDLPAGASINQVREFMKKNSSLGIGDITSESRRYLSYVGQATSYNSGKAVMMDLYTKVQKKLGLSRREFVEKDNHKYVKEFFDALLRNSALPMDALIKSVSAKYGLTVEKK", "aa_hexdigest": "861552b9f77c70e476f66df59e83ebd3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P8H0", "query_cov": 0.8675496688741722, "subject_cov": 1.0234375, "identity": 0.557, "score": 140.0, "evalue": 2.01e-40, "valid": false, "product": "Bacterial protein of uncharacterized function (DUF885)", "uniref50_id": "UniRef50_A0A3B0P8H0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "product": "Bacterial protein of uncharacterized function (DUF885)" }, "genes": [], "id": "AHCKFKLKGB_776", "locus": "GCA013367975_00767" }, { "type": "cds", "sequence": "contig_1", "start": 448311, "stop": 448430, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGTTGACTTCTCAACAGCACATGGTATAACAAACTTCTTTACTGCTAAAACAGAAGCAGAAGTTACAGATGACATGATTAAACAAATTAAAGACCTACACAATGGTGTATATTGA", "aa": "MPVDFSTAHGITNFFTAKTEAEVTDDMIKQIKDLHNGVY", "aa_hexdigest": "b0173a6c01a784096c3861479ffec60b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4385.8811000000005, "isoelectric_point": 4.857937812805175 }, "id": "AHCKFKLKGB_777", "locus": "GCA013367975_00768" }, { "type": "cds", "sequence": "contig_1", "start": 448515, "stop": 448631, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGACACCACAACCAATTAATGTATGCTCAAAAGTATCTATCATCAGTTGGAGAATTTGGTAAATATAAATTAGGAAATGTATTCCACTACACTTCATATGTTGAAGGTTGA", "aa": "MMGHHNQLMYAQKYLSSVGEFGKYKLGNVFHYTSYVEG", "aa_hexdigest": "064ced3e7916d6f765f037258ca96559", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4415.979300000001, "isoelectric_point": 8.093570518493653 }, "id": "AHCKFKLKGB_778", "locus": "GCA013367975_00769" }, { "type": "cds", "sequence": "contig_1", "start": 448662, "stop": 448976, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGAAATATGTTGATGAATTAACAAAATTCAATTATAATCAATTACTAAGTGAAAAAAATAAAGCTACAAAACATGGTTCGATAACAAACGAAGAATTCTATTATGGTGGATTGTCAGCATTTAAAGCATATGATCAATTTAAGAAGACTACTCAAAATTATGGACGTAAATTCTTTGCAAATCCCGTTCCTGATTATGATATTCAAACATATGCCTTTTCTAGAAGAGAATTTTCAGGTGTTGGAGCTTACGACTCAAGCATTAAAAAATTCATGTTTAATGTTGACCCATACTTCTCATTACCAAAATGA", "aa": "MKKYVDELTKFNYNQLLSEKNKATKHGSITNEEFYYGGLSAFKAYDQFKKTTQNYGRKFFANPVPDYDIQTYAFSRREFSGVGAYDSSIKKFMFNVDPYFSLPK", "aa_hexdigest": "0063a34bc859dc1db63559b5f7975501", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12212.57939999999, "isoelectric_point": 9.323176002502443 }, "id": "AHCKFKLKGB_779", "locus": "GCA013367975_00770" }, { "type": "cds", "sequence": "contig_1", "start": 449089, "stop": 449265, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCTTTTTCATCACGAATTACAACAGTTTCTTCTTTTATTTCTTCTGGTCCAATACCATCAGCATCATATTTAATTTTGGCTTCTCATTTTTTGCCTTTTCCAACAATTAAATCAGCTGCTTGGTTAGCAATTTTCTTTGAGTTTTCAGCAGATTTCTTTGTTGTTTCATAA", "aa": "MFPFSSRITTVSSFISSGPIPSASYLILASHFLPFPTIKSAAWLAIFFEFSADFFVVS", "aa_hexdigest": "ea56eb80e1eb0db2d5e379dfa934a12c", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6382.334099999998, "isoelectric_point": 6.505302238464354 }, "id": "AHCKFKLKGB_780", "locus": "GCA013367975_00771" }, { "type": "cds", "sequence": "contig_1", "start": 449178, "stop": 449729, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGAAAGTTTTCTCAAAAATGGTAATTAATGCTTTTGTAGGAAGTTTATTTCAAACACAACTAACTAATTTTGTAAATGATAAGTCTAAAAACGAAATTTCAGTTGTAGAATTAATTAATTCTTCTACCTTGCCAACAGCCAAAAAAGAATTATTAAAATATTATGCAACTACATATTACAATGCTGCAACTCATGGATTGGGTGAAGACATTAAAGAATTAAAACTTTATAAAGAAAATAAAGTTAATGAAAAAGAATTGTCTATTGATGCAAAAAATGCTAGTGGGGAAATTGTTAAATTATATGGTTTAGGATTAACTGAAAAAGACCTAAATCAAAGAAATGTTGGTTTGGGTTTTGCTGAAGGAGATGCTACCGTAAATGGTCAATCAATGTATCGTCAAATTCTAAAGATGGCTACAACTTCTGATTTAACAGATGATCAAGTAAATAATATTGGTTATGAAACAACAAAGAAATCTGCTGAAAACTCAAAGAAAATTGCTAACCAAGCAGCTGATTTAATTGTTGGAAAAGGCAAAAAATGA", "aa": "MSKVFSKMVINAFVGSLFQTQLTNFVNDKSKNEISVVELINSSTLPTAKKELLKYYATTYYNAATHGLGEDIKELKLYKENKVNEKELSIDAKNASGEIVKLYGLGLTEKDLNQRNVGLGFAEGDATVNGQSMYRQILKMATTSDLTDDQVNNIGYETTKKSAENSKKIANQAADLIVGKGKK", "aa_hexdigest": "76260c2f9ed00d98cdb408235766e8b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20157.659099999993, "isoelectric_point": 8.842048454284669 }, "id": "AHCKFKLKGB_781", "locus": "GCA013367975_00772" }, { "type": "cds", "sequence": "contig_1", "start": 449928, "stop": 451040, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAAACTAGAATTCTAATTTCATTAGGTTCAATTTTTGGCGTAGCAGCCTTAATGCCTATGGCAGCATCATGTACACATACAAATAATGATGAATTAAACAAAAAAATTGAATCTTTAGAAAAGCAATTAAATGAAGCAAAAGCAAATTCTCAAAAAGATCAAGCACAAATTAATGATCTTATGAAGCAATTGCAAGAAACAAAAAATAATTCAAAAACAATTGAAGAAAAAGAAAAATTAGTTAAAAAATTGCAAGAAGATTTAAATAAAATCCAACAAGAAAGAACAGCTAAATATCAAGCAGAATTAAATGCTGCAAACAAAGCTATTGTTGAAATTCCTAAGAAATATGAAGAATTAAATGAAAAAAATGATGATCTTAAAGATCAATTAAGTAGCTTAGAGACCAAGCTATTGTCTTCTGAAAGCAAATTTAAAAATTCAGGGAAAAAAATAGAAAGTTATTTGAAAAAATCAACAGAAATAGAAAATAACATTAAAGAATCTAAAACTAAATTAGTTGAATTAAGAAAAGCCTTGGATGATTTAAATAAAAACCTAGAAACATTAGAAAAACAAAATTCTGATGGCACAAAAAATAATGAAATATCATCAAAAAAGGAAGAAATTAAAGCTAAGAATTTAGAAATTAAACAAGAAGAAGAAAAGTTTGAAGGTCTTCAAACAGAATTAAAAACTCAAAAAGATAAAATCATTGAAGAAACTAATAAAAAGTCAAAACTTGAAATTGAAGTTGCAAATTTGACAACAAAAAAAGAAAATATTTCTAAAGAAATTGAAGCTAATCAAAAAGAATTAGATGCACTAGACAAAGAATATGACGATATTGTTAAAAAGAGCGATGAATTAAGCAATTTACTTTCAAAAGAAAATGATAATTCACCAGCCAGCCAAGAAGCAGCCGCAAGACATATTTTAGATTTAAAAGGTGATCTTGAATCTGAATTAAAGAAAGAAAAATTAAATGGAATAAGTGCACCTACTGCTGAACAATCAAAGAAAATTTCTGAAATTTATGGTAAATATATTGAAAAAATTTCAAAAATAAATGATGCCTCATTAACGAGCGATTCTTTAGCATGA", "aa": "MKKQTRILISLGSIFGVAALMPMAASCTHTNNDELNKKIESLEKQLNEAKANSQKDQAQINDLMKQLQETKNNSKTIEEKEKLVKKLQEDLNKIQQERTAKYQAELNAANKAIVEIPKKYEELNEKNDDLKDQLSSLETKLLSSESKFKNSGKKIESYLKKSTEIENNIKESKTKLVELRKALDDLNKNLETLEKQNSDGTKNNEISSKKEEIKAKNLEIKQEEEKFEGLQTELKTQKDKIIEETNKKSKLEIEVANLTTKKENISKEIEANQKELDALDKEYDDIVKKSDELSNLLSKENDNSPASQEAAARHILDLKGDLESELKKEKLNGISAPTAEQSKKISEIYGKYIEKISKINDASLTSDSLA", "aa_hexdigest": "71fb0505926e4cc2087c94aae2f20d74", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 41972.89400000005, "isoelectric_point": 5.757016944885254 }, "id": "AHCKFKLKGB_782", "locus": "GCA013367975_00773" }, { "type": "rRNA", "sequence": "contig_1", "start": 451405, "stop": 451509, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8823529411764706, "score": 58.8, "evalue": 2.6e-09, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "CGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCTTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTT", "id": "AHCKFKLKGB_38", "locus": "GCA013367975_00774" }, { "type": "cds", "sequence": "contig_1", "start": 451720, "stop": 451872, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCCTCATTTCTATTATTATTTCTATGTATATAAATATGCATTAGGATATATCGTAGCAAATGTATTTTTCCAAAAATACAAACAAGAAGGCCAAGAAGCATTAAAAAACTATATAAATAAATTTTTAAGTGCCGGAGACATCGATTGA", "aa": "MVPHFYYYFYVYKYALGYIVANVFFQKYKQEGQEALKNYINKFLSAGDID", "aa_hexdigest": "ccab9e932d67397b07838c4ee1e3418c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6061.8657, "isoelectric_point": 7.928853797912598 }, "id": "AHCKFKLKGB_783", "locus": "GCA013367975_00775" }, { "type": "cds", "sequence": "contig_1", "start": 452029, "stop": 452427, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTATGAATATCCAGAAGTTGTTAAAAAAGCTATTGATGAACGTTGGGTTGATTATGTCAATGTTCCATCTAAACGAAGCGGAGCTTATTCTATAGGTGGGGCAGCAGGAATGAAAAAAATTTATATATTGATGAATTTTGATGGAACATTGGAATCAGTCAATACATTATGCCACGAAATGGGACATTCAATGCATTCGTATTTTTCTAATAAATGCCAATCTCCACTTAGAAGCTCTTATCCAATATTTTTAGCAGAAATTGCTTCGATATTTAACGAATTATTACTAAATGATTATTTAATAAGCAAATCAAAAAATATTCAACAACAATTTTATTTACTTGATTCAAGCATTAATGATTTTATTGGCACAGTTTTGCGCCAAACGATGTGA", "aa": "MPYEYPEVVKKAIDERWVDYVNVPSKRSGAYSIGGAAGMKKIYILMNFDGTLESVNTLCHEMGHSMHSYFSNKCQSPLRSSYPIFLAEIASIFNELLLNDYLISKSKNIQQQFYLLDSSINDFIGTVLRQTM", "aa_hexdigest": "12b4d54c82e222d8b047438011431e97", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15077.136299999984, "isoelectric_point": 6.267885780334471 }, "id": "AHCKFKLKGB_784", "locus": "GCA013367975_00776" }, { "type": "cds", "sequence": "contig_1", "start": 452512, "stop": 452994, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAACTTGATCATAAATTGTCGGATGATGTTGAAAATTATTTAAATGATACTGCAAGTGGTTCGCCTGTACCTGAAGATATTTTTTCAATAATAAGCGATAGTGAAGTTGATTTTGGCAATGTTTTAATTAAAAATAAAAAAATAAAAATAACCGAAGGAAATAGAATAGCTTTATTAAAAAATAAAGATGAACAAGTTAGAAAAGATGTTTATTTTAATTATTTAAATGGATATTTAAAAAATAAACAATCATTAGCAAGATTGCTATATCAACATATTAAAGAAATATCAGTTAATGCTTTATATAGAAAATATAATTCATCTCTTGAATCTATTTTGTTGCAAGATCATGTTGATAAAAAGTTATTGAACATTATTTATGATGCAGTTCAAAAACATATGTATATATTTAAAAAATATAACAATGCTAAAAAACAATTTTTTAAAGCTAAATTCAATAAAAAATTAGAAAAATGA", "aa": "MQELDHKLSDDVENYLNDTASGSPVPEDIFSIISDSEVDFGNVLIKNKKIKITEGNRIALLKNKDEQVRKDVYFNYLNGYLKNKQSLARLLYQHIKEISVNALYRKYNSSLESILLQDHVDKKLLNIIYDAVQKHMYIFKKYNNAKKQFFKAKFNKKLEK", "aa_hexdigest": "8cd7040db18df7e979293f3522fcdff7", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18823.412900000003, "isoelectric_point": 9.33084774017334 }, "id": "AHCKFKLKGB_785", "locus": "GCA013367975_00777" }, { "type": "cds", "sequence": "contig_1", "start": 453043, "stop": 453306, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCAAAAATAATTGAAATAAAAGATAGCAAATACAATTCATTAGACCAATATGTTGAATATATTGAGCTTTCAAAGAAATTCTTAATTTTAGGCAATAGAATTTCTAATTATTTGGATAATAAATTAAATATAAATATTGTTGATCCCACAATAAATAAAACAATTTCATTATTTGAAACAAAATCCCAAGAATATGCTAAAAAGTTAGGTTCAGAAGCCAATAGAATTTTTCAACATAAAGACAAAATCAAAACATGA", "aa": "MYSKIIEIKDSKYNSLDQYVEYIELSKKFLILGNRISNYLDNKLNINIVDPTINKTISLFETKSQEYAKKLGSEANRIFQHKDKIKT", "aa_hexdigest": "224e375b41de36fb76ea8eb75d953e69", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10248.67349999999, "isoelectric_point": 9.273406410217284 }, "id": "AHCKFKLKGB_786", "locus": "GCA013367975_00778" }, { "type": "cds", "sequence": "contig_1", "start": 453417, "stop": 453932, "strand": "-", "frame": 2, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0003999", "GO:0009116", "KEGG:K00759", "RefSeq:WP_012855642.1", "SO:0001217", "UniParc:UPI0001BC2388", "UniRef:UniRef100_D1J8F6", "UniRef:UniRef50_Q9RFQ2", "UniRef:UniRef90_D1J8F6" ], "nt": "ATGGATTTAAAAAAGTATATAAGAACTGTTGATGACTTTCCACAAAAAGGCGTTAAATTTAAAGATATTTCGCTTTTATTAGCCAATGGACAAGCACTACATTACACAATTGATGAAATGGCAAAATTAGCAGAAGGTGCAGATATTATTGTGGGTCCTGATGCAAGAGGATTTTTATTTGGAACACCAGTAGCTGCTAAATTATCAAAACCATTTATTATGGTAAGAAAAAAAGGCAAATTGCCCGGCGAAGTTATAAGCTGCGATTATGGACTTGAATATGGTAGAAATATTTTAGAAATTCAAAAAGGTTTAGTTAAACCAGGACAAAAAGCCGTCATAGTTGACGATGTTTTGGCAACTGGGGGTACATTGGAAGCAATTACTAAATTATTAACTGAACAAGGTGTAATTATCAGTAGAATAATTGTTTTAATGGAATTAGATGGCTTTAATTCTCATGAAAGATTGCCATACGATATAAAAAGTTTAATCTATATTAATAAAGGGGAATAA", "aa": "MDLKKYIRTVDDFPQKGVKFKDISLLLANGQALHYTIDEMAKLAEGADIIVGPDARGFLFGTPVAAKLSKPFIMVRKKGKLPGEVISCDYGLEYGRNILEIQKGLVKPGQKAVIVDDVLATGGTLEAITKLLTEQGVIISRIIVLMELDGFNSHERLPYDIKSLIYINKGE", "aa_hexdigest": "e1df95e8030d659ee05d448dda0526e2", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2388", "ncbi_nrp_id": "WP_012855642.1", "uniref100_id": "UniRef100_D1J8F6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2388", "RefSeq:WP_012855642.1", "UniRef:UniRef100_D1J8F6" ] }, "ips": { "uniref100_id": "UniRef100_D1J8F6", "uniref90_id": "UniRef90_D1J8F6" }, "psc": { "uniref90_id": "UniRef90_D1J8F6", "gene": "Apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_Q9RFQ2", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0003999", "GO:0009116" ] }, "pscc": { "uniref50_id": "UniRef50_Q9RFQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RFQ2" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "AHCKFKLKGB_787", "locus": "GCA013367975_00779" }, { "type": "cds", "sequence": "contig_1", "start": 454146, "stop": 454385, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAATTAATTTTTTTGCATTAGGTGGTTTAGATGAAAACGGCAAAAATTGTTATGTTTTAGAAATAAATAACAAACTATTTGTGATTAACTCAGGAACTAAAATTCCTATTAACTCGCATAACGGTATTGATACATTAATTTGCAATTACGAATTTTTAGAAAAACGTCAAAAAGATATAGAAGGATTATTTTTGACTGATGTTAGAAATGAAACGTTTTCTGCTATTCCTTGA", "aa": "MNKINFFALGGLDENGKNCYVLEINNKLFVINSGTKIPINSHNGIDTLICNYEFLEKRQKDIEGLFLTDVRNETFSAIP", "aa_hexdigest": "9227c53fcb884f46403e9711cc4e8abd", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8967.135899999994, "isoelectric_point": 5.212613105773924 }, "id": "AHCKFKLKGB_788", "locus": "GCA013367975_00780" }, { "type": "cds", "sequence": "contig_1", "start": 454392, "stop": 454766, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAAAACCTAAAAATATATACTAGTGCTTTTAACAAAATTGTTTTATTAGATAGAATTTCTAAATATAATATAGGTTCGAATACATTTGAAGTTAAAACAATAAATAAAAAAACAGCATTTGGCGGAGTAAATGTAACTCCTTTTGATCTAGCCGGAGGTTTGCCTGGACAACTTGGGTTCAATTTTGAAACCCCAATTAGCAATATCTTGTTTATGACAAATTTTGTTGATGGTGATTTGGGTCCTTATGGTAAAACCGATTTGCAACAAATTCAACAAATAATTAATAACAAAAAGGAATTACAGCTTTTAATAATGGATAGTTCAAGAAGCGCTTTTCCTGGACGTTCAATTGAAAAACTTTGA", "aa": "MKIKNLKIYTSAFNKIVLLDRISKYNIGSNTFEVKTINKKTAFGGVNVTPFDLAGGLPGQLGFNFETPISNILFMTNFVDGDLGPYGKTDLQQIQQIINNKKELQLLIMDSSRSAFPGRSIEKL", "aa_hexdigest": "c211546d96dcb2aa95dca5bf55cfb2a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13803.850099999985, "isoelectric_point": 9.559452629089353 }, "id": "AHCKFKLKGB_789", "locus": "GCA013367975_00781" }, { "type": "cds", "sequence": "contig_1", "start": 454845, "stop": 455801, "strand": "+", "frame": 3, "gene": "rnjA", "product": "mRNA degradation ribonuclease J1/J2", "db_xrefs": [ "COG:COG0595", "COG:J", "SO:0001217", "UniRef:UniRef50_A0AAJ5NJJ8", "UniRef:UniRef90_D1J8F7" ], "nt": "ATGATAACTGCACATGAAATTCTAATACTTGCAAAACGTTATAACAGACCGGTTATAACTTATGGTTTAGCTTATTCTCAATTAATAAAACTTGTTGAAAAGGTAAATCCAAATTCTCAATTTCCGGAATTCGTTGAATATCAAACTGCAAATAATGTTAAAAATGCCGTTATTCTTGTAACAGGTGCAATAGATAGACTTTACACAAGATTTGCAAGAATTTCTAGCGATAATGATGTTTATTTAAAATTGAAAAATGATGATGCAGTCATTATAATTGCTCCACCAGTTAACGGGTTGGAAGTAAATTATGCAATAACACTAGATGATATTGCTAGACATACTGCTAATTTAATTGAACTAGGCAATGATCAATATTATTCATGCAATCCTGCTAAATTAGATATTTTTAATATTATTAAAGCATTAAAACCTAAATTCTTTATACCAATTCAAGGTCTATACCGTTACCAAGTTGTAGCAACTCAAGTTGCCAGAAGCGCAGGTATGAACATTAGTAACTGCTTAGTGTTGCAAAACGGAAAGGTGGCTGAATTCAACGATACAAATTTAATTTCTCAAAAGCACGGAATTAGAAATGTAGGTGATGTTATTATTGACGGCTATGGTATTGGCGATATTTCTCATGAAGTTATTAATGAAAGAGAAAACCTAGCAAGAGATGGTGTAATTGCTCTTAGCTCATTAATAGATTATAAAAAGAAGAAACCAATCAATGAACTTCAAATTTCTTCATATGGAATAATTACGAAAGATAATAAAGAAGTTATTCACAAAATAATTAATGATTTATTCAAAAAAGAATTCGAAAATAAACAAACAAAAGAAATAAATTTAAAGGAAATTCAAGAAAAATTAAGAAAATCAATTAAGAGAAAAATCGCCAAAACTATTGACAAAGAGCCAATGATTGTAATAACTTTATATGAAATTTAA", "aa": "MITAHEILILAKRYNRPVITYGLAYSQLIKLVEKVNPNSQFPEFVEYQTANNVKNAVILVTGAIDRLYTRFARISSDNDVYLKLKNDDAVIIIAPPVNGLEVNYAITLDDIARHTANLIELGNDQYYSCNPAKLDIFNIIKALKPKFFIPIQGLYRYQVVATQVARSAGMNISNCLVLQNGKVAEFNDTNLISQKHGIRNVGDVIIDGYGIGDISHEVINERENLARDGVIALSSLIDYKKKKPINELQISSYGIITKDNKEVIHKIINDLFKKEFENKQTKEINLKEIQEKLRKSIKRKIAKTIDKEPMIVITLYEI", "aa_hexdigest": "6d31e7ac111009a0ff6504458270a352", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnjA" ], "truncated": "5-prime", "pseudogene": { "start": 454545, "stop": 455801, "observations": { "mutation": [ 454764 ], "stop": [ 454764 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8F7", "query_cov": 1.0, "subject_cov": 0.5771324863883848, "identity": 1.0, "score": 571.0, "evalue": 3.86e-201, "gene_start": 1, "gene_end": 318, "reference_start": 234, "reference_end": 551, "reference_sequence": "MNKINFFALGGLDENGKNCYVLEINNKLFVINSGTKIPINSHNGIDTLICNYEFLEKRQKDIEGLFLTDVRNETFSAIPWLLMKIKNLKIYTSAFNKIVLLDRISKYNIGSNTFEVKTINKKTAFGGVNVTPFDLAGGLPGQLGFNFETPISNILFMTNFVDGDLGPYGKTDLQQIKQIINNKKELQLLIMDSSRSAFPGRSIEKLWVSRKIEYKFKETSDNQRIIVGLYDEDMITAHEILILAKRYNRPVITYGLAYSQLIKLVEKVNPNSQFPEFVEYQTANNVKNAVILVTGAIDRLYTRFARISSDNDVYLKLKNDDAVIIIAPPVNGLEVNYAITLDDIARHTANLIELGNDQYYSCNPAKLDIFNIIKALKPKFFIPIQGLYRYQVVATQVARSAGMNISNCLVLQNGKVAEFNDTNLISQKHGIRNVGDVIIDGYGIGDISHEVINERENLARDGVIALSSLIDYKKKKPINELQISSYGIITKDNKEVIHKIINDLFKKEFENKQTKEINLKEIQEKLRKSIKRKIAKTIDKEPMIVITLYEI" }, "paralog": false, "identity": 0.9952153110047847, "score": 767.0, "evalue": 5.42e-281, "description": "Internal stop codon at 454764. Nonsense mutation around 454764.", "psc": { "uniref90_id": "UniRef90_D1J8F7", "gene": "RnjA", "product": "mRNA degradation ribonuclease J1/J2", "uniref50_id": "UniRef50_A0AAJ5NJJ8", "cog_id": "COG0595", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8F7", "UniRef:UniRef50_A0AAJ5NJJ8", "COG:COG0595", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAJ5NJJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5NJJ8" ], "product": "Ribonuclease J" } }, "id": "AHCKFKLKGB_790", "locus": "GCA013367975_00782" }, { "type": "cds", "sequence": "contig_1", "start": 456436, "stop": 456846, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E31DEDA", "UniRef:UniRef90_UPI001E31DEDA" ], "nt": "ATGATATTCTCTCCAAAAAGCAAAAAGAACAAGGAAAAACTTCAAAATCAAAGCGATTTAAAAATAAAGGAAGAGGATATTGAAATAATCGATAACAAAGAAGTCGTAAATAGCAATATTCAAATTTTAGAGGCTAATTCTAACTCAAATAAAAATATAGACTCAACACAAAATTCAAACCAAAATAACAATCTTGATTTAGATCCTTTTGATGATGAAAAAGAAATTGAAGAAGATTCAAAACAAAAAGACAACAATGAGCCAGAGCCAAAAGAAGCTATTGACTCACCAAGCAATGAAAATTCTATAGATCCTACACTACAAGAACCAGATAATTCAAATAAAAATAAAGAGGTTAAAAAAGAAGATAAGATTTATAAAATCATTGAAGACGAAGAAGATTTATTCTAA", "aa": "MIFSPKSKKNKEKLQNQSDLKIKEEDIEIIDNKEVVNSNIQILEANSNSNKNIDSTQNSNQNNNLDLDPFDDEKEIEEDSKQKDNNEPEPKEAIDSPSNENSIDPTLQEPDNSNKNKEVKKEDKIYKIIEDEEDLF", "aa_hexdigest": "6317b1ef50351a4e2f9813c4dc1a9637", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 456163, "stop": 456846, "observations": { "mutation": [ 456241 ], "stop": [ 456241 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E31DEDA", "query_cov": 1.0, "subject_cov": 0.5991189427312775, "identity": 0.809, "score": 146.0, "evalue": 1.61e-41, "gene_start": 1, "gene_end": 136, "reference_start": 92, "reference_end": 227, "reference_sequence": "MGSVIYYTIHQVEHNSKNFFTKYFSSWFVDFSNSSNYLLPQKYTPGVIGTFINSILNIPGKKLGFSIAYIISIVLLSLSVISFFISNSFFKMIFSPKSKKNKEKLQNQSDLKIKEEDIEIIDNKEVVNSNIQILEANSNSNKNIDSAQNSNQNNNLDLDPFDDEKEIEEDSKQKDNNELEPKEAIDSPSNENSIDPTPQKPDNSNENKEVKKEDKIYKIIEDEENLF" }, "paralog": false, "identity": 0.8634361233480177, "score": 329.0, "evalue": 3.28e-116, "description": "Internal stop codon at 456241. Nonsense mutation around 456241.", "psc": { "uniref90_id": "UniRef90_UPI001E31DEDA", "uniref50_id": "UniRef50_UPI001E31DEDA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E31DEDA", "UniRef:UniRef50_UPI001E31DEDA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E31DEDA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E31DEDA" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15735.794499999989, "isoelectric_point": 4.330983543395997 }, "id": "AHCKFKLKGB_791", "locus": "GCA013367975_00783" }, { "type": "cds", "sequence": "contig_1", "start": 457214, "stop": 458164, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D7B81", "UniRef:UniRef90_UPI00215D7B81" ], "nt": "ATGAATAGTAAAAAAAGTAAAAAAATTGTGGTTGCACTTTCACTAACATTGATTGCTTCTGCCATCACGATACCCGTTGTAATTTCGTCCTTAATTGAAAATAAGAATAATTGGGCTAATAAATACTATAGCCAAAGATCAGAAGCTAGTCATCTAGTTAATTTAATAGAAAAAAATAAGCATTTTTATTATTTTGCCCTAGAAGATCAAAACAATTTAAATAAGTTATTAAGTGAAATTAATTTAACCAGCAAAAATAGTTCTGCTGATTTTAAAAATTCATATAATCTACTTAACAAGACAAATCTTGATATCAACAAAAAAATAAATATTAATAAAAAAGAATTTGACAAAACTTATTCTGATATTTTGCTTTTAAGCTTGCAATTACAAGATTTATTCAATAAAATTATTGATTTAAAATTGAATAACAATATAAAAGAAGATGTTGATGGATTATTACAAAGAGTTGAAAAAGATTTAAATAAATGTAGAAAAATAGATGAAAACATTAATCTTATTGACATTAAAGAAATATACGCACATAGTTCAAAAACAATCATTGATGTTTCAAATCAATTAAATTCATATTATAAATTAATTAACAGCAAAGATAAAACATATAAAGGCATTGTTTCAGAAATAAAAGATCTTAAAAAAGATTATTTCAACAAAATTGAAGATGGAGAAGAATATTACAAATTATATGGCAATGAAATAAACCATCTTAAAAAGAATTTTAAAAATTTCCAATCAGTTGAAGATTACAACAATTATATAAATTACGCCAATTCAAAAATAAAGGAAATTAAAGAAGAAATTGTTGAACATAGAAAGCTAAGCCAAAAGATAAAAAAAGATGGAAATGATATTTTAAATAATGATTATGGTAAAAGTGCTTTAATAAGAAATTCATACTTCCAAGAACCAAAATCATTTTTATTAATGTGA", "aa": "MNSKKSKKIVVALSLTLIASAITIPVVISSLIENKNNWANKYYSQRSEASHLVNLIEKNKHFYYFALEDQNNLNKLLSEINLTSKNSSADFKNSYNLLNKTNLDINKKININKKEFDKTYSDILLLSLQLQDLFNKIIDLKLNNNIKEDVDGLLQRVEKDLNKCRKIDENINLIDIKEIYAHSSKTIIDVSNQLNSYYKLINSKDKTYKGIVSEIKDLKKDYFNKIEDGEEYYKLYGNEINHLKKNFKNFQSVEDYNNYINYANSKIKEIKEEIVEHRKLSQKIKKDGNDILNNDYGKSALIRNSYFQEPKSFLLM", "aa_hexdigest": "8dad780ef29dc4a90097fbfcfc7f95ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00215D7B81", "query_cov": 0.9683544303797469, "subject_cov": 0.8116710875331565, "identity": 0.971, "score": 515.0, "evalue": 6.14e-182, "valid": true, "uniref50_id": "UniRef50_UPI00215D7B81" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D7B81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D7B81" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 36982.72300000001, "isoelectric_point": 9.111590766906737 }, "id": "AHCKFKLKGB_792", "locus": "GCA013367975_00784" }, { "type": "cds", "sequence": "contig_1", "start": 458186, "stop": 458314, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATAAAGGCAAAATTCATTAATGAACGTCCTAAAAAAATAAAAGAATTCCTAAAGCAATATGAAGAAAGGCAAGAATACTTTTATAGAATTAATGAAAATGTTCCAAACCTTATTTACACATGA", "aa": "MLIKAKFINERPKKIKEFLKQYEERQEYFYRINENVPNLIYT", "aa_hexdigest": "08884dbdb0bffc462ade886a691c41e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5339.1732, "isoelectric_point": 9.396992301940916 }, "id": "AHCKFKLKGB_793", "locus": "GCA013367975_00785" }, { "type": "cds", "sequence": "contig_1", "start": 458546, "stop": 458950, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D844A", "UniRef:UniRef50_UPI00215DA8CC", "UniRef:UniRef90_UPI00215D844A", "UniRef:UniRef90_UPI00215DA8CC" ], "nt": "ATGAAATTAGATGACATCCATTTTGACCCGGAACAAGATATTAGATATTTACACTACCATTTTGGAAGTCTCATAACTCAAAAGGTTAATGAAATAATTAGGTATTATAACCAAAAAGCAAAAACCTTATTAGAAAAAATAAAAAACAATAATAGTTATGAAACACAAAAAAATAGAATAGAAAAATTACTTGCTAAAAATATTACTTATGTAAATAATTTTAGAGGGGATTTCAACGATTACGACAAATACTATTGGTTAACATATAAATTTCAAAAAGACAACAATGGCGATATAATCCTTTCGCCAAATAGCAAAATCCCTGATTTTTATAAAAATGAAATTGAAGTAGCTCAATACCTACATGAACTTGATGAAATACTTGATAATTTAAAAAACATTTAA", "aa": "MKLDDIHFDPEQDIRYLHYHFGSLITQKVNEIIRYYNQKAKTLLEKIKNNNSYETQKNRIEKLLAKNITYVNNFRGDFNDYDKYYWLTYKFQKDNNGDIILSPNSKIPDFYKNEIEVAQYLHELDEILDNLKNI", "aa_hexdigest": "6b28db80aad4ebd5009659ad3eecff69", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00215DA8CC", "query_cov": 1.0, "subject_cov": 0.8701298701298701, "identity": 0.925, "score": 253.0, "evalue": 1.19e-84, "valid": true, "uniref50_id": "UniRef50_UPI00215DA8CC" }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA8CC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA8CC" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 458283, "stop": 458950, "observations": { "deletion": [ 458307 ], "stop": [ 458313 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D844A", "query_cov": 1.0, "subject_cov": 0.6036036036036037, "identity": 0.948, "score": 259.0, "evalue": 4.31e-86, "gene_start": 1, "gene_end": 134, "reference_start": 89, "reference_end": 222, "reference_sequence": "MKTFQTLFYTWVIYNNKIRIIKEFFFKDKKENEEFNSFYYKDKDIDNQLNSLLNEEREFKNRQERLVRLFYDKVIQDPKTNEIDEEKLMKLDDIHFDPEQDIRYLHYHFGSLITQKVNEIIKHYNEKAKALLEKIKNNNSYETQKNRIEKLLAKNITYVNNFRGDFNDYDKYYWWTYKFQKDSNGDIILSPNSKIPDFYKNEIEVAQYLHELDKILDNLKNI" }, "paralog": false, "identity": 0.9369369369369369, "score": 371.0, "evalue": 9.95e-133, "description": "Internal stop codon at 458313. Frameshift due to deletion around 458307.", "psc": { "uniref90_id": "UniRef90_UPI00215D844A", "uniref50_id": "UniRef50_UPI00215D844A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D844A", "UniRef:UniRef50_UPI00215D844A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D844A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D844A" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16292.128599999991, "isoelectric_point": 6.073894309997558 }, "id": "AHCKFKLKGB_794", "locus": "GCA013367975_00786" }, { "type": "cds", "sequence": "contig_1", "start": 459193, "stop": 459327, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGAGAAAGAAATTGATTGCTTTATCTGTGGCTTTTGGACTTTTAGCGCCTCTTTCAGGAACTATTATGATTTCGTGCAAAAACGAAAAAAGTAATGCTGAATATCAAAAAGAACTACAAAATAAATGA", "aa": "MKMRKKLIALSVAFGLLAPLSGTIMISCKNEKSNAEYQKELQNK", "aa_hexdigest": "5294e7c6498948119217fb1b901816a4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4926.885100000001, "isoelectric_point": 9.807978248596193 }, "id": "AHCKFKLKGB_795", "locus": "GCA013367975_00787" }, { "type": "cds", "sequence": "contig_1", "start": 459454, "stop": 459987, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04778.17" ], "nt": "TTGAAGAATTTTATTGCAAAAATTAATGAAACTGTGCAAGATATAAATGCACAAATTTCAAAATTAAAAAACACAAATGGAAATGCAAGCCCAGAAAACAACAGCCAAAATGATTTAAAAGTAGAATTAGAAATAGCAACTAAAAAACTTAACATGCTACTTTCATCAAATGAAGGTAAAAACATCTCAATAAAAGAAGAAGCTAAAAAAATATTAAACGCCGCAAATAAAGCGTTAGAAAAATCAAATGAACAAGAATACAAAAACCAAATTAAGAAAGTAAATGAAACTATCAATAAAATTGAAGAAGAAATATCAAATTTAGAAAAAGGCCCAAAATCAAAAGAATTTACTAAATTAAAAGAATTAAGAGAAAAGCTAACAAATTACTTAGATAATAATTTCTCTGAATTTGCTTATAAAGATTTAAGAGCCGAAATCAAAGAATTAATTCAAAAACAAGAAAACGTTTCAATTGTTTCAAATGAAAAAGAAATAAAGTCAGCAATTTCACAAATAAATGATGCCTCTTGA", "aa": "MKNFIAKINETVQDINAQISKLKNTNGNASPENNSQNDLKVELEIATKKLNMLLSSNEGKNISIKEEAKKILNAANKALEKSNEQEYKNQIKKVNETINKIEEEISNLEKGPKSKEFTKLKELREKLTNYLDNNFSEFAYKDLRAEIKELIQKQENVSIVSNEKEIKSAISQINDAS", "aa_hexdigest": "3819289a73021ee946d267f68a3603c9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 130, "aa_cov": 0.7288135593220338, "hmm_cov": 0.9923076923076923, "evalue": 1.6391570911052269e-07, "score": 26.658113479614258, "start": 48, "stop": 176 } ], "seq_stats": { "molecular_weight": 20172.547300000006, "isoelectric_point": 7.736946296691895 }, "id": "AHCKFKLKGB_796", "locus": "GCA013367975_00788" }, { "type": "cds", "sequence": "contig_1", "start": 460102, "stop": 460944, "strand": "+", "frame": 1, "gene": null, "product": "P75 protein precursor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RLP9", "UniRef:UniRef90_UPI000CD67922" ], "nt": "TTGAATACCTCAATTTTTGAAAACATTGAAACAACAAATGAAAGAAATCTATTAAACAAATATATTAAAACAAATTTAACTAAAATTTTAGATGTAGAAAAACAAAAATATATTGAAGAAGTTAAAAACACTATAAGCTCTTTGTTGAACAAATATAATAATAATATAAATAACAATAAAGATCAAATTTATGAATTAAGTCGAAACATAGAACAATTAAATAAATTAAATCAAATAGAAAATAATTATGTTATTGACAAAGTCAAACTACTAAATCTATTAAATAAAGCAAATACTATAAAAAATGAAGGTTCATTTGCTAAAAAAGCAATTTTACTAGATGAGTTAAAAGACAGCGTTTCGGAATTTCTTGAATTTTATTTGCAATTAAGCCATTATCTAAGAATCTTTTTTGATAAAAATAACGACATTATTAAATTATTACCAGCAGATTCACAAACAAAAATTAATAATTATCTAAACGAAATTAAAAAAATTGCGGAAAAAAATAGTCAAGCCAAAGGAAACGAACCAACATTTGATGAATATATTTCAGCCTTTAAAGCACAAGAAAAAATTTTTAAAATTATAAAATCTAGTGATCTAAAATCAGCAGATGTTAACATATTAGAAAAATTGACAAATAATTTAAAAGAAATTAAAAAAGAAAAGAAAAATGATTACAACTTGGTTGTTACTTCTTATTTAAAAGAAAAAATAGAAGAAGCAATAACAAAAAATGAATCAATAATTGCCAAAAAATCTAATATCGAAAGTCAAAATATTACTGAAACGTTTGTTTTATTGCAAAAATTAGAACTTGAATTTGCATATATTAATTGA", "aa": "MNTSIFENIETTNERNLLNKYIKTNLTKILDVEKQKYIEEVKNTISSLLNKYNNNINNNKDQIYELSRNIEQLNKLNQIENNYVIDKVKLLNLLNKANTIKNEGSFAKKAILLDELKDSVSEFLEFYLQLSHYLRIFFDKNNDIIKLLPADSQTKINNYLNEIKKIAEKNSQAKGNEPTFDEYISAFKAQEKIFKIIKSSDLKSADVNILEKLTNNLKEIKKEKKNDYNLVVTSYLKEKIEEAITKNESIIAKKSNIESQNITETFVLLQKLELEFAYIN", "aa_hexdigest": "597d74299c1327c05c287a089a3365fc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 459802, "stop": 461145, "observations": { "mutation": [ 459985, 460942, 461044 ], "stop": [ 459985, 460942, 461044 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000CD67922", "query_cov": 1.0, "subject_cov": 0.43143297380585516, "identity": 0.946, "score": 481.0, "evalue": 3.29e-165, "gene_start": 1, "gene_end": 280, "reference_start": 304, "reference_end": 582, "reference_sequence": "MKMRRKLIALSVAFGLIAPISGALMISCKNEKSNAEYQKELQNKWTELKNLLNSNSFKEVESKLDLSKLNALKEEYKTINSQSNVSTLKNFIAKINETAQDINAQISKLKNTNGNTNPENNSQNNLKVELEIATKKLDMLLSSNEGKNISIKEEAKKILNAANKALEKSNEQEYKNLIQKVNETIDKIEKEILNLEKGPKSKEFTKLKELREKLTNYLDNNFSEFAYKDLRAEIKELIQKQENVSIVSNEEEIKSAISQINDAFWIASIKKSILDKQFLYLENKPKSELDIKFLAAYDELSQALNTSIFENIETTNERNLLNKYIKTNLTKILDVEKQKYIEEVKNTISSLLNKYNNNINNNKDQIYELSRNIEQLNKLNQIENNYVIDKAKLLNLLNKANTIKNEGSFAEKAILLDELKDSVSEFLEFYLQLTHYLRIFFDKNNDIIKLLPADSQTKINDYLDEIKKITEKNSQTRGNEPTFDEYISAFKAQEEIFKIISSDLKSADVNILEKLTNNLKEIKKEKKNDYNLVVTSYLKEKIEETITKNESIIAKKSNVESQNIIETFALLQKLELEFAYINWTELNLIAKIKNKLELLIKINKIDSNEEAKILKPWTDSFKSSKKPEIFELENILKELSKSLKELINK" }, "paralog": false, "identity": 0.9485458612975392, "score": 805.0, "evalue": 7.63e-295, "description": "Internal stop codon at 459985, 460942, 461044. Nonsense mutation around 459985, 460942, 461044.", "psc": { "uniref90_id": "UniRef90_UPI000CD67922", "product": "P75 protein precursor", "uniref50_id": "UniRef50_Q9RLP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000CD67922", "UniRef:UniRef50_Q9RLP9" ] }, "pscc": { "uniref50_id": "UniRef50_Q9RLP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RLP9" ], "product": "p75 protein" } }, "id": "AHCKFKLKGB_797", "locus": "GCA013367975_00789" }, { "type": "cds", "sequence": "contig_1", "start": 460878, "stop": 461099, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCTAATTCAAAAATTTCTGGTTTTTTTGAACTATTAAATGAATCTGTTCAAGGCTTTAATATTTTTGCTTCTTCGTTCGAATCTATTTTATTGATTTTTATTAACAATTCTAATTTATTTTTAATTTTAGCAATCAAATTTAATTCAGTTCAATTAATATATGCAAATTCAAGTTCTAATTTTTGCAATAAAACAAACGTTTCAGTAATATTTTGA", "aa": "MFSNSKISGFFELLNESVQGFNIFASSFESILLIFINNSNLFLILAIKFNSVQLIYANSSSNFCNKTNVSVIF", "aa_hexdigest": "52d7010d89e1a04565810b6caa75c6fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8198.333399999994, "isoelectric_point": 5.983065605163574 }, "id": "AHCKFKLKGB_798", "locus": "GCA013367975_00790" }, { "type": "cds", "sequence": "contig_1", "start": 461328, "stop": 461645, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D96F05" ], "nt": "ATGATTAATGATATGTTTGAAAAAACTGATCAAGATAGAGTTTATAAAATAAAAACATTCTTGCCTAGTGGAAATGCGCATGATGAAAGAAAATTATATTTTGATAAAAGTCTTCCTTTATTTAAATTATTTGACAAAGTAAATGTAAATTTTAATACATTTTTAGGGGGAGCAAAAACTAAAGGAATTTATAAGCAAGATGGGAAATTAAGATTTGAAGGATTTATTCAATTTTATGACCCAGAAATTCCTTATTATAAATTTATTGGATCGGTGTACCCCATAACCATTGATGAAATAACAATTAAAGCCCAATAA", "aa": "MINDMFEKTDQDRVYKIKTFLPSGNAHDERKLYFDKSLPLFKLFDKVNVNFNTFLGGAKTKGIYKQDGKLRFEGFIQFYDPEIPYYKFIGSVYPITIDEITIKAQ", "aa_hexdigest": "f3c9d3a3003f83533faac7f24bdf57fa", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D96F05", "query_cov": 0.819047619047619, "subject_cov": 1.0, "identity": 0.767, "score": 137.0, "evalue": 1.6e-40, "valid": false, "uniref50_id": "UniRef50_UPI0034D96F05" }, "pscc": { "uniref50_id": "UniRef50_UPI0034D96F05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D96F05" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12364.08149999999, "isoelectric_point": 8.72980899810791 }, "id": "AHCKFKLKGB_799", "locus": "GCA013367975_00791" }, { "type": "cds", "sequence": "contig_1", "start": 461748, "stop": 462194, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCACCAAGCGATGAATTTTTAAAAATTGAAGAAGAATTTCAAGACCAAAAGAAGCAAATTGAATCTAAACTAAATGAAATTTGCTCTCTAAATTTCAATAAAACATATAGTGGACAATTAATTGAATCAAATGAATCCATAAAGAAGGCAATTAATAAAAAAGTTAATGATGATGTTTATGGCGAATACCCAGGATATGTAGAAAAATATTATTATAGAAAAGCCTATAATGCAAGAAATAAAGAAGATTGTAAGAAATATATAAACCAACTTAAAAATATTTATAAGATAGTTGATATGTACGCTAATTATTTAAAAGAAGTATTCAAATATGTTGAATCAAAAGATGCAAAATTAAGTGATGAAATAAAACAAAAGATAATGGAAGAAGATGTTATAGAAATTACCAAAACTAGTAGAAGCAAAATTGAATATTTAAATTGA", "aa": "MPPSDEFLKIEEEFQDQKKQIESKLNEICSLNFNKTYSGQLIESNESIKKAINKKVNDDVYGEYPGYVEKYYYRKAYNARNKEDCKKYINQLKNIYKIVDMYANYLKEVFKYVESKDAKLSDEIKQKIMEEDVIEITKTSRSKIEYLN", "aa_hexdigest": "5000abd8d6df127bfeea56b5c534627d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17708.904300000006, "isoelectric_point": 6.427375984191894 }, "id": "AHCKFKLKGB_800", "locus": "GCA013367975_00792" }, { "type": "cds", "sequence": "contig_1", "start": 462501, "stop": 463283, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAGGCTAAGCAAGAATTACAAAATCTTATTGATTTTAGCGCTGACCAAGGAATTGAATCTTCAAAAGCAAAAGAAATTTTAGATTCAACAAACATTGAAGAAAATTCTTCAATTAGTGAAGTTAAAGAAAAAACAGACAAAATTAATAAAGCTAAACAAGATCTTCAAAATTTAATAGAAGATACCAAAAATAAAGCAAAAAATGAGTTTGATATTAAAAAACAAGAATTGAGAGCACTTCTAGATTCAATAGACAATAAGATTAATAAACAAGTTGAAGAAGAAAAATATAAGGATATTGAAGTTAAAGATAATGATTTAGTTAATGACATTAAAAATAAAACTAATGAAATTTCAAAAGCAATTGAATCATTGAATAAAAAAATTAATGAACAAAAAAATGAACAAGAATTTGAACAAGCAAAACAAGATTTTGAAATTAAAAAACAAGAATTGAAAACATTAATTGAATCTGAAAAGGCAAGAAATATTGATAAATCTGAAGAACAAAATGTATTAAATACTACAAATGTTGCTGAATCATCAAATATTTCAGACATTAAATCAAAAACAAAGATTATTGAAGAAGCAATCAATTCATTGACTAAAAAAATTAATGAACAAGAAAATAAACAAGAATTTGAACAAGCAAAACAAGATTTTGAAAAAATAAAAAAAGAACTACAAGAATTAATTGCAACCCCTGATGCAAAGGAAGTAGAAAACATTGATGATATAAACAAAACCTTGAATGAAGCAAATATCGCCCAAGATTGA", "aa": "MKKAKQELQNLIDFSADQGIESSKAKEILDSTNIEENSSISEVKEKTDKINKAKQDLQNLIEDTKNKAKNEFDIKKQELRALLDSIDNKINKQVEEEKYKDIEVKDNDLVNDIKNKTNEISKAIESLNKKINEQKNEQEFEQAKQDFEIKKQELKTLIESEKARNIDKSEEQNVLNTTNVAESSNISDIKSKTKIIEEAINSLTKKINEQENKQEFEQAKQDFEKIKKELQELIATPDAKEVENIDDINKTLNEANIAQD", "aa_hexdigest": "e0426208ad65679302f7762931a7546c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30012.02540000003, "isoelectric_point": 4.8624849319458 }, "id": "AHCKFKLKGB_801", "locus": "GCA013367975_00793" }, { "type": "cds", "sequence": "contig_1", "start": 463455, "stop": 463640, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAAATTTATCATTACAAATTTTAGCGTTGTGTGTAGCGGCGGGCTCTGTGCCTGTTTTAGCATCTAAATGTACCGTAACTGTAAAAGTTAAAGAAAAGTTTAATGCTCTAAAATCACAACTTCAAAATTTAATTTCAAAACTAAGTGATGAAGAACAACAAGAACTTAATGAATGA", "aa": "MKKKNLSLQILALCVAAGSVPVLASKCTVTVKVKEKFNALKSQLQNLISKLSDEEQQELNE", "aa_hexdigest": "4a25dd07e169d330eef575d4d9523fbb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6700.840199999999, "isoelectric_point": 9.064206504821776 }, "id": "AHCKFKLKGB_802", "locus": "GCA013367975_00794" }, { "type": "cds", "sequence": "contig_1", "start": 463873, "stop": 464421, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGATCAAAAAGATTTTAATATTATAGATAGTGAAATAGCAGCATTTGGCAATGTAAAAAAGATCTTTGATGAAAATATAAACAATATAAACGATATTTGTAAAGAAGTAATTAAACAGGAATTTTTAGAAAAAAATTCAAATAATAAAAGAATTGAAGAAGCTAAAAAACAATACGAAATAGTAAGAGATTCTAATGAAAGAATTACTGCCGAAAAAAAGCTAAAAAAAATATCAGGAAGGAAATTTGCAAATTGCATAATTTTTATACTTTTCTTTATTCTAATAGGTTTTTGTTTTTTTTCATTTTTTGCTAACAATAAGAAAATAATTAAATCATTTGAAGAGTTTAGAAATAATGAACAATTAAAAATTGATGAAGCGTTATATAAAGCAAGACAAGCATACGTTGAAACCTTTTCATTACAAACCCTTGAAGATATAAAAAATAAAATTTTAAACAAAATGGGAATAAAAAAAATATCTCAATACGATGCTGAAGTTGGTGATTTTTCGCGTAGTTTTATTTATGATAAAAATTTTTGA", "aa": "MEDQKDFNIIDSEIAAFGNVKKIFDENINNINDICKEVIKQEFLEKNSNNKRIEEAKKQYEIVRDSNERITAEKKLKKISGRKFANCIIFILFFILIGFCFFSFFANNKKIIKSFEEFRNNEQLKIDEALYKARQAYVETFSLQTLEDIKNKILNKMGIKKISQYDAEVGDFSRSFIYDKNF", "aa_hexdigest": "d5ed3f2256007474034293ad67f8d72c", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21553.509799999996, "isoelectric_point": 8.605062675476077 }, "id": "AHCKFKLKGB_803", "locus": "GCA013367975_00795" }, { "type": "cds", "sequence": "contig_1", "start": 464845, "stop": 465753, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA032", "UniRef:UniRef90_UPI00215DA032" ], "nt": "ATGAATCAAGAAAACATGCCGGTGTTTTCGATGATAAAAAATGGCAATTGGTTGGTTTCTAAAAATACGATTCAATCAAGTTCCTTATTATTTGAACAAGTTCAGGATTGGATGTTAAGTGTTTTGGATTTAACTGAAGAACTAACAGTTGACTTTGTCACGGAATTTTTAAGTTCAAAAATTATTGAATGTTTATTGCCAATATTTAATTTTATTTCCTTAGTATATACAAATAAGTATATTGCAACTGAAAATTTAGAAAAAACAGAAAATAGATATGAACAAGACCTTGTTGTTAAACAAAATAACAATATTCAAAATTGCCCTCTAGACATTCACTCATATTATAGAGACATGACATTATATAGTGGGTTTTATAAATTTAACAAGCATGCAGATATAACACCAATTTGCGAATTTAAAAATCTTTATAATGACCCAGAATCTTTGATAATATCGGCTACATTTGATTTGATTTCATATACAGCTAGAGACGCAATTGCAATTGAATATACACACGGTTACGCCGTTGAAGTACCATATGTGGCATACGATGAATTTAGAGAACATAAAGCAATAATTGTTGTTCCTTATAATATAAAAACAGAAAACGCCAAAATATTTGCAAATAGTCTTATTGCTAATAATATTTATGTAAGTGACGATCGCATAGATGAAGAATATAGCAAAATTATTCAAAAAAATAAAATTTCATTTAATTTAGAATCATTAAAGGACTTTGATGAATTTAAGAAAAATATTAGGCTTATAGATGAATTATTTAGTCAGATTCCTGAAATAAAAAAGATTCCAATACTACTTGATAATAGAGGAATGGCTTTCTTTATAAATGAGCCAAAATTATTTGACATCAATTTATTAGTTAAAAAGCTTAATAATTTTTATTAA", "aa": "MNQENMPVFSMIKNGNWLVSKNTIQSSSLLFEQVQDWMLSVLDLTEELTVDFVTEFLSSKIIECLLPIFNFISLVYTNKYIATENLEKTENRYEQDLVVKQNNNIQNCPLDIHSYYRDMTLYSGFYKFNKHADITPICEFKNLYNDPESLIISATFDLISYTARDAIAIEYTHGYAVEVPYVAYDEFREHKAIIVVPYNIKTENAKIFANSLIANNIYVSDDRIDEEYSKIIQKNKISFNLESLKDFDEFKKNIRLIDELFSQIPEIKKIPILLDNRGMAFFINEPKLFDINLLVKKLNNFY", "aa_hexdigest": "728730eec0803c70227f94ddffb6f30f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 464545, "stop": 465753, "observations": { "mutation": [ 464560, 464572 ], "stop": [ 464560, 464572 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215DA032", "query_cov": 1.0, "subject_cov": 0.6425531914893617, "identity": 0.99, "score": 590.0, "evalue": 4.16e-210, "gene_start": 1, "gene_end": 302, "reference_start": 169, "reference_end": 470, "reference_sequence": "MGIKKISQYDAEVGDFSRSFIYDKNFWNFNSISQYKLRNSYIYDIFYTCKWMKNVVTKASITVTEEDANGNTYWETYWAYHTEPTPVVEYFRSLTIPSNYKQKLYFEIIENNRSEKEVKRLLKNEGFHLENKEVYQNFDFAFSDDQKIDFLTYFDVRTQQNLINYAQYMNQENMPVFSMIKNGNWLVSKNTIQSSSLLFEQVQDWMLSVLDLTEELTVDFVTEFLSSKIVECLLPIFNFISLVYTNKYIATENLEKTGNRYEQDLVVKQNNNIQNCPLDIHSYYRDMTLYSGFYKFNKHADITPICEFKNLYNDPESLIISATFDLISYTARDAIAIEYTHGYAVEVPYVAYDEFREHKAIIVVPYNIKTENAKIFANSLIANNIYVSDDRIDEEYSKIIQKNKISFNLESLKDFDEFKKNIKLIDELFSQIPEIKKIPILLDNRGMAFFINEPKLFDINLLVKKLNNFY" }, "paralog": false, "identity": 0.9875621890547264, "score": 799.0, "evalue": 3.35e-295, "description": "Internal stop codon at 464560, 464572. Nonsense mutation around 464560, 464572.", "psc": { "uniref90_id": "UniRef90_UPI00215DA032", "uniref50_id": "UniRef50_UPI00215DA032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215DA032", "UniRef:UniRef50_UPI00215DA032" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA032" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35436.106400000004, "isoelectric_point": 4.861575508117675 }, "id": "AHCKFKLKGB_804", "locus": "GCA013367975_00796" }, { "type": "cds", "sequence": "contig_1", "start": 465841, "stop": 466398, "strand": "+", "frame": 1, "gene": "lemA", "product": "Protein LemA", "db_xrefs": [ "COG:COG1704", "COG:M", "GO:0005886", "KEGG:K03744", "SO:0001217", "UniRef:UniRef50_P43054", "UniRef:UniRef90_P43054" ], "nt": "ATGTTATTTGATTCAAGAACCCCACAATCTAGTGAAGGATTTAAACCAAACGTAGATAATAGCATTAAAAAACCAATTCCAACAGGTGTTGAAAAATTCTTTTTTATATTATTTTTTATACTTACAATAGGAATTTTCTATTTTGTATATGTAGGAAGAAAAAATGAATTAATGCGTGATCAAAACGAAATTCAAAATGCAAGTTCTCTAATACAAGCAGCAGAAAAAAGGAGACGTGCAGTTCTAATTAAAATGATGGATAGTTTAATTGGCTATAAAAATTTTGAAAATGAAACATTAACTAAAATAACTCAATATAGATCAAAATTATCAAATATCGATGTAGACAAAACTTCTCCCGTTGAATTGAAATCACAAATTGACAGCATCAGAGGTGCTTTAAACTTTCAATTTGAACAATACCCAGATCTTAAAGCAAGCAAATTGTATTTACAATTTTCAACTGAAATTTCTATGCAAGAAGATGAAATTTATGCAACAATAAGAAACTATAATATGATTGCAACATCATTTAATTCAAAAATATATACATTTTGA", "aa": "MLFDSRTPQSSEGFKPNVDNSIKKPIPTGVEKFFFILFFILTIGIFYFVYVGRKNELMRDQNEIQNASSLIQAAEKRRRAVLIKMMDSLIGYKNFENETLTKITQYRSKLSNIDVDKTSPVELKSQIDSIRGALNFQFEQYPDLKASKLYLQFSTEISMQEDEIYATIRNYNMIATSFNSKIYTF", "aa_hexdigest": "181baf512e89f9176479d877cbeb4d28", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_P43054", "query_cov": 1.0, "subject_cov": 0.8447488584474886, "identity": 0.995, "score": 357.0, "evalue": 3.14e-124, "valid": true, "gene": "lemA", "product": "Protein LemA", "uniref50_id": "UniRef50_P43054", "cog_id": "COG1704", "cog_category": "M", "kegg_orthology_id": "K03744", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P43054", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43054" ], "product": "Protein LemA" }, "genes": [ "lemA" ], "id": "AHCKFKLKGB_805", "locus": "GCA013367975_00797" }, { "type": "cds", "sequence": "contig_1", "start": 466586, "stop": 466720, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAAATAGAAGAAATACATAAATATAATGCCGATAATATTCAAATATTAGAAGGTTTAGAAGCCGTAAGAAAAAGACCCGGCATGTACATAGGTTCAATAGGGTTCAAGGGTTTGCACCATTTGCTATGA", "aa": "MDKIEEIHKYNADNIQILEGLEAVRKRPGMYIGSIGFKGLHHLL", "aa_hexdigest": "8d4f09ee56f4b74af8dfcccb6640f0f0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5048.840599999998, "isoelectric_point": 6.81854190826416 }, "id": "AHCKFKLKGB_806", "locus": "GCA013367975_00798" }, { "type": "cds", "sequence": "contig_1", "start": 466751, "stop": 466996, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGGCGGGTTTTGCTACTGAAATTAAAATTAAATTGTATCCAAATAATGTGATAGAAGTTGAAGACAATGGTCGTGGAATGCCCACAGGAATTCACTCTGGAACTAAAAAGTCAGCTGTCGAAACAATTTTAACCGTGCTACATGCTGGGGGTAAATTTGATGGATCAAATTATAAAGTTTCCGGAGGATTACATGGTGTTGGTGCATCAGTTGTTAATGCATTAAGTAGTGAATTTGAAGTATGA", "aa": "MAGFATEIKIKLYPNNVIEVEDNGRGMPTGIHSGTKKSAVETILTVLHAGGKFDGSNYKVSGGLHGVGASVVNALSSEFEV", "aa_hexdigest": "db565570dd8e84d5374519d63bf2fa94", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D3B81C", "query_cov": 1.0, "subject_cov": 0.84375, "identity": 0.593, "score": 100.0, "evalue": 4.92e-26, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_Q706I4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 76, "aa_cov": 0.9012345679012346, "hmm_cov": 0.8157894736842105, "evalue": 4.1693799359956163e-11, "score": 38.64239501953125, "start": 5, "stop": 77 } ], "seq_stats": { "molecular_weight": 8404.4133, "isoelectric_point": 6.25185718536377 }, "id": "AHCKFKLKGB_807", "locus": "GCA013367975_00799" }, { "type": "cds", "sequence": "contig_1", "start": 467060, "stop": 468370, "strand": "+", "frame": 2, "gene": "gyrB", "product": "DNA gyrase subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P43053", "UniRef:UniRef90_P43053" ], "nt": "TTGGAAGTAATTGGAAATGTTCCTGAAGTTGAAACAGGAACAACAATTAAATTTCACCCTGACTATACCATAATGGAAAAAGAAAATTTTGACTTTGATACAATTATTGACCACTCCAAACAAATTGCTTATTTAAACAAAGGTTTGAAAATAACCGTTGAAAATGTTGAAAAAAATATCATCAAAGTTTTTTGTTTTGAAGGTGGACTAATAGACTATGTCAAAGAACTAAACAAAGGTAAAAAATTAATAGTTCCCGAAGTTATTTATGCAGAAGGAGTTTTTAACGATAAAAACTTTACAAATGGACAAGATGTAATAGTAGAAGTTGCAATGCAATATAATGAAGCCTACACAAATAGTATTGTTTCTTATGCAAACAATATTCAAACAATTGATGGTGGAACACATGAACAAGGTTTCTATGATGCATTAGTAAGAATTTACAATAATTACGCCGAAACAAATAAACTATTTAAAACTAGCTCAGAAAAAATAACAAGAGAAGATGTTAAGGAAGGTTTAGTAGCAATCATTTCTATTAAACACACAGACCCAATTTTTGAAGGTCAAACTAAAGGAAAATTAGAAAATAAAGATGCAAGAATTGCCACAAATAAAATTCTTTCAGACTCGCTAGAACGTTATTTGAATGAAAACCCAGAAATTGCAAGAGCAATAATCGAAAAGTGTCTTCTTTCGCAACACACAAGGCTTCTTGAAATAAAGGCTCGCGAAGCTTCTAGAAAAGGTAATGGTTTAGATTTAGGTAATCTTCCTGGAAAATTAGCAGACTGTTCATCGAAAAATGCAGAAATTAGAGAATTATTTATTGTCGAAGGTAATTCGGCCGGAGGTTCTGCTAAAATGGGTAGAGATCGTTCTATTCAAGCTATTTTGCCTCTACGTGGTAAGGTTATAAATGCAGAAAAAAATTCGTTTGCTTCTGTCTTGTCAAATAAAGAAATTGCAACAATGATTCACGCCTTAGGCACGGGAATAAATACAGAATTTGATATTAACAAATTAAAATATCACAAAATTATCATTATGACAGATGCCGACGTTGATGGAGCGCACATTACTACTTTATTATTGACATTCTTTTACCGTTATATGAAACCTTTAATTGAATATGGATTTGTTTATTTGGCACAACCTCCGCTATACAAAATAACTAGTGGAAAAAATGTTGAATATGCATACAATGATTTACAAAAAGAACAAATCATGGCAAAATTAGAAGATAAAAGAAATGTTGCTATTCAACGTTACAAAGGTCTTGGTGAAATGGACCCAGAACAACTATGA", "aa": "MEVIGNVPEVETGTTIKFHPDYTIMEKENFDFDTIIDHSKQIAYLNKGLKITVENVEKNIIKVFCFEGGLIDYVKELNKGKKLIVPEVIYAEGVFNDKNFTNGQDVIVEVAMQYNEAYTNSIVSYANNIQTIDGGTHEQGFYDALVRIYNNYAETNKLFKTSSEKITREDVKEGLVAIISIKHTDPIFEGQTKGKLENKDARIATNKILSDSLERYLNENPEIARAIIEKCLLSQHTRLLEIKAREASRKGNGLDLGNLPGKLADCSSKNAEIRELFIVEGNSAGGSAKMGRDRSIQAILPLRGKVINAEKNSFASVLSNKEIATMIHALGTGINTEFDINKLKYHKIIIMTDADVDGAHITTLLLTFFYRYMKPLIEYGFVYLAQPPLYKITSGKNVEYAYNDLQKEQIMAKLEDKRNVAIQRYKGLGEMDPEQL", "aa_hexdigest": "2da834d5bfb7958efa860297b16a027a", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI000D1D9E0B", "query_cov": 0.8555045871559633, "subject_cov": 0.8923444976076556, "identity": 0.517, "score": 389.0, "evalue": 1.98e-129, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_F5VUB3" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 466760, "stop": 468532, "observations": { "mutation": [ 466994, 468368 ], "stop": [ 466994, 468368 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43053", "query_cov": 1.0, "subject_cov": 0.6728395061728395, "identity": 0.995, "score": 855.0, "evalue": 8.1e-310, "gene_start": 1, "gene_end": 436, "reference_start": 159, "reference_end": 594, "reference_sequence": "MDKIEEIHKYNADNIQILEGLEAVRKRPGMYIGSIGFKGLHHLLWEIVDNSVDEAMAGFATEIKIKLYPNNVIEVEDNGRGMPTGIHSGTKKSAVETILTVLHAGGKFDGSNYKVSGGLHGVGASVVNALSSEFEVWVKRDGKLHYQQFRNGGIPVKPLEVIGNVSEVETGTTIKFHPDYTIMEKENFDFDTIIDHSKQIAYLNKGLKITVENVEKNIIKVFCFEGGLIDYVKELNKGKKLIVPEVIYAEGVFNDKNFTNGQDVIVEVAMQYNEAYTNSIVSYANNIQTIDGGTHEQGFYDALVRIYNNYAETNKLFKTSSEKITREDVKEGLVAIISIKHTDPIFEGQTKGKLENKDARIATNKILSDSLERYLNENPEIARAIIEKCLLSQHTRLLEIKAREASRKGNGLDLGNLPGKLADCSSKNAEIRELFIVEGNSAGGSAKMGRDRSIQAILPLRGKVINAEKNSFASVLSNKEIATMIHALGTGINTEFDINKLKYHKIIIMTDADVDGAHITTLLLTFFYRYMKPLIEYGFVYLAQPPLYKITSGKNVEYAYNDLQKEQIMAKLEDKRNVAIQRYKGLGEMDPEQLWETTMDPETRKMLQVQIDDAAICDTVFATLMGEEIEPRHDFIQENAKYANNIDI" }, "paralog": false, "identity": 0.9949152542372881, "score": 1159.0, "evalue": 0.0, "description": "Internal stop codon at 466994, 468368. Nonsense mutation around 466994, 468368.", "psc": { "uniref90_id": "UniRef90_P43053", "gene": "gyrB", "product": "DNA gyrase subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P43053", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0005694", "GO:0005737", "GO:0005524", "GO:0003677", "GO:0034335", "GO:0046872", "GO:0006265", "GO:0006261" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43053", "EC:5.6.2.2", "UniRef:UniRef50_P43053", "COG:COG0187", "COG:L", "GO:0005694", "GO:0005737", "GO:0005524", "GO:0003677", "GO:0034335", "GO:0046872", "GO:0006265", "GO:0006261" ] }, "pscc": { "uniref50_id": "UniRef50_P43053", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43053" ], "product": "DNA gyrase subunit B" } }, "id": "AHCKFKLKGB_808", "locus": "GCA013367975_00800" }, { "type": "cds", "sequence": "contig_1", "start": 468380, "stop": 468532, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACCCAGAAACTAGAAAAATGCTCCAAGTTCAAATAGATGATGCAGCAATTTGTGATACAGTGTTTGCTACATTAATGGGAGAAGAAATAGAACCTCGTCATGATTTTATTCAAGAAAACGCAAAATACGCAAATAATATAGATATCTAA", "aa": "MDPETRKMLQVQIDDAAICDTVFATLMGEEIEPRHDFIQENAKYANNIDI", "aa_hexdigest": "21e2080d0bfa91ac8070ba9604ef6d73", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5754.417799999999, "isoelectric_point": 4.176893043518065 }, "id": "AHCKFKLKGB_809", "locus": "GCA013367975_00801" }, { "type": "cds", "sequence": "contig_1", "start": 468534, "stop": 468719, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAGCCCCTAACTAATCAAGGTTTCACAAACAAAGTATTTTATGATGATGAAACTAATAGATTTATAAAAATAAAATCATATGATGGATTTAACCATAAAAGCGATGCATTCTTATTAAATAATTTAGATTTTTGTCCAAAGATATTTGTTGATAATAAAAAAGAACTTCAAACCGAATGA", "aa": "MLKPLTNQGFTNKVFYDDETNRFIKIKSYDGFNHKSDAFLLNNLDFCPKIFVDNKKELQTE", "aa_hexdigest": "beb625d91cc544b720c1c5a13551fe00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7235.125100000001, "isoelectric_point": 6.551284980773925 }, "id": "AHCKFKLKGB_810", "locus": "GCA013367975_00802" }, { "type": "cds", "sequence": "contig_1", "start": 468750, "stop": 468974, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGATGATATTTTAAAAACTATTGGAAAAAATTTAATCACTTTGCATAATTCAAAATTGAAATTTTATAAAGAAAATCAAATTGCTAGAAGATTTAATATTTATAGAAAAAAAATTTCTAGTTTAAATAGAAAAATTCCAATCCTAGATAAATACTATAAAAAAATTAATTTGTTTTTAAGAAATATTGACAATTCTGCCCCTGTCCATAATGACCTATGA", "aa": "MTDDILKTIGKNLITLHNSKLKFYKENQIARRFNIYRKKISSLNRKIPILDKYYKKINLFLRNIDNSAPVHNDL", "aa_hexdigest": "19fc795c88b81a826147d5e7c4888cec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8862.315299999995, "isoelectric_point": 10.167453956604003 }, "id": "AHCKFKLKGB_811", "locus": "GCA013367975_00803" }, { "type": "cds", "sequence": "contig_1", "start": 469035, "stop": 469190, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGATGTACATTTTGACCTTGCCTATTTTATTGAATCAAGCAATCTAAATGAAAAACAAGAAAAAGTTTTTTTAGATGCTTATGGTGATGATTTTGAACCCAAATATTTATTTATTCACAAAATTTTAGTAAATGCTTTAATTGTGCTATGA", "aa": "MGDVHFDLAYFIESSNLNEKQEKVFLDAYGDDFEPKYLFIHKILVNALIVL", "aa_hexdigest": "7b8a2a13d8a2be708d1059d9310e35ac", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5963.7613999999985, "isoelectric_point": 4.547426414489746 }, "id": "AHCKFKLKGB_812", "locus": "GCA013367975_00804" }, { "type": "cds", "sequence": "contig_1", "start": 469285, "stop": 469602, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAAGAGATTGTTGTTGTGGTTGCAAGTCAGCTGAGTTCTGCACATGCAAAAATAAAAAATCATGTATTCCTAATAATTTATATGAAAAAATTAGGAATGTTTTTGGTTATGAAGTTTTTGAAAAACTTAATAATTTAAGACCTTATTTTGATGATTTACATAGTAGCACATATATTGGAAAACTAGAAGATGTTTGGGTTCAAATAAGAATTCCTTCTGACAATAAAATAAATTATGACAACGAAACAAAACTAGTTGAAAAATTCAAAGATTATTTTTATTACAAAGATGGATATATTATAAAAAAATGA", "aa": "MLKRDCCCGCKSAEFCTCKNKKSCIPNNLYEKIRNVFGYEVFEKLNNLRPYFDDLHSSTYIGKLEDVWVQIRIPSDNKINYDNETKLVEKFKDYFYYKDGYIIKK", "aa_hexdigest": "af67f32cde0ef22a1f2c0660224c381c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12595.385399999988, "isoelectric_point": 8.620857429504397 }, "id": "AHCKFKLKGB_813", "locus": "GCA013367975_00805" }, { "type": "cds", "sequence": "contig_1", "start": 469915, "stop": 470223, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTATTCCAAAAGAAGATATAATTGATTTCTTTAAATTAGACCCTTCTGTATTTGATGATTTTGTTTTTTTAATGAGAGTTTACAATGAGGCAATGTATTTAAATGATTATTCAAAAAATAATTCCAAATCGTTAAGCCCTTTTGATTCAAAGAGTTTATATAGCAACAAGGATTTCTTAGAATTAAATAGATTCATTGTTCAAAAAAATCATAATAACTTTGACAATAAATTAAATATTTCTAAAATTGAAAAATTTTATTTTGTTCCACTTTGTGTTTACGAAGATGAAGATAGAACCATATGA", "aa": "MGIPKEDIIDFFKLDPSVFDDFVFLMRVYNEAMYLNDYSKNNSKSLSPFDSKSLYSNKDFLELNRFIVQKNHNNFDNKLNISKIEKFYFVPLCVYEDEDRTI", "aa_hexdigest": "3a1720ea33e21b6fe6d9403def826757", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12230.716999999988, "isoelectric_point": 4.881014442443848 }, "id": "AHCKFKLKGB_814", "locus": "GCA013367975_00806" }, { "type": "cds", "sequence": "contig_1", "start": 470632, "stop": 470784, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATGTCCAAAAGACTTTTATAAATATAGAATTATTGTTCACTTTACAGCATATTTATATAACAAATTATTAAACACTGATTTTAATGCGGCTAAGGTTAACACTAAAAGAATAAATGAAATATTTGAAAAGTTAAACATTAAAGACTAA", "aa": "MICPKDFYKYRIIVHFTAYLYNKLLNTDFNAAKVNTKRINEIFEKLNIKD", "aa_hexdigest": "4d6d525a10d2f40f07be44431cd0d210", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6072.0634, "isoelectric_point": 9.416461753845216 }, "id": "AHCKFKLKGB_815", "locus": "GCA013367975_00807" }, { "type": "cds", "sequence": "contig_1", "start": 470795, "stop": 471277, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MHO_3790", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43056", "UniRef:UniRef90_P43056" ], "nt": "ATGAAAAAAGGCAAAGCAAAAGAAACAAAAACTATTGACGTCGACAAGCAAGAAAAAAAATCAGTTAGAATTCGTAGAGTAAAAACCGGATTTCTATTTTCAGGAATTTTAATTCCTTTAGTTTGCGTTGTCGCAATACCACTTTCATTAAACAAGAATTATTCATTAAAACGCACCAAATATTTTACTGACGATAGAACTTTATATTCAATTGACAAAAACACTATAAAAGAAAATAACAAAACTTATAAAATACTTACCCTAACATCTAAAAGTCTTAATGTTTCTGATATCATGAGCGAAAGCGCAAGATATATGAATATTCCGCAGAAATCGTTTAATAATTCCGTTACATATTCAAATAAAATCAATATTAGAACAAATACCAACTTGTTAAAAAATACAAAAATTAATGAAACAAAGGTTACAAAAATAACAAATAGTAACCAAACTATTTTTAAATTAGATTTAATTCAACTATGA", "aa": "MKKGKAKETKTIDVDKQEKKSVRIRRVKTGFLFSGILIPLVCVVAIPLSLNKNYSLKRTKYFTDDRTLYSIDKNTIKENNKTYKILTLTSKSLNVSDIMSESARYMNIPQKSFNNSVTYSNKINIRTNTNLLKNTKINETKVTKITNSNQTIFKLDLIQL", "aa_hexdigest": "0fdb0d6c68ca5ffee3953c831ff9ffa6", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 470795, "stop": 471580, "observations": { "mutation": [ 471275 ], "stop": [ 471275 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43056", "query_cov": 1.0, "subject_cov": 0.5970149253731343, "identity": 0.988, "score": 290.0, "evalue": 1.93e-97, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MKKGKAKETKTIDVDKQEKKSVRIRRVKTGFLFSGILIPLVCVVAIPLSLNKNYSLKRTKYFTDDRTLYSIDKNTIKENNKTYKVITLTSKSLNVSDIMSESARYMNIPQKSFNNSVTYSNKINIRTNTNLLKNTKINETKVTKITNSNQTIFKLDLIQLWNEINSKSNNKISEFDFLSNIESINNAMTYSYIKNNKLFYFDNKQGYSGYIIQKFFENFDVEIRKENLKNKNVNVLEAFISSTIELNESLTEIKKITFDTKITLKYEI" }, "paralog": false, "identity": 0.9885057471264368, "score": 504.0, "evalue": 3.13e-184, "description": "Internal stop codon at 471275. Nonsense mutation around 471275.", "psc": { "uniref90_id": "UniRef90_P43056", "product": "Uncharacterized protein MHO_3790", "uniref50_id": "UniRef50_P43056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43056", "UniRef:UniRef50_P43056" ] }, "pscc": { "uniref50_id": "UniRef50_P43056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43056" ], "product": "Uncharacterized protein MHO_3790" } }, "id": "AHCKFKLKGB_816", "locus": "GCA013367975_00808" }, { "type": "cds", "sequence": "contig_1", "start": 471356, "stop": 471601, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTACTCTTATATAAAAAATAACAAGCTCTTTTACTTTGACAATAAGCAAGGATATTCTGGATATATTATTCAAAAATTCTTTGAAAATTTTGATGTTGAGATTCGTAAAGAAAATCTTAAAAATAAAAATGTTAATGTTTTAGAAGCTTTTATTTCTAGCACTATTGAATTAAACGAATCATTAACCGAAATTAAAAAAATTACTTTTGATACTAAAATTACATTAAAATATGAAATATAA", "aa": "MTYSYIKNNKLFYFDNKQGYSGYIIQKFFENFDVEIRKENLKNKNVNVLEAFISSTIELNESLTEIKKITFDTKITLKYEI", "aa_hexdigest": "3dc4f00d8a053d96587fe4510e7deb2b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9721.00909999999, "isoelectric_point": 7.864836692810059 }, "id": "AHCKFKLKGB_817", "locus": "GCA013367975_00809" }, { "type": "cds", "sequence": "contig_1", "start": 471644, "stop": 472063, "strand": "-", "frame": 3, "gene": null, "product": "DUF885 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "nt": "ATGTTACAATACTTTGGAGCATTGAACGAAGCACAATTACGTAATATGCGTAGAGCAGTTGACCCTGCATACCATGGACAAGGAATTGAAGCAAAATCTGATTTACCTGCAAATGCATCAATTAATGATGTAAGAAGATTTATGTCAGCAAACTCAGCATTAGGTGTTGGTGACATTATGTCTGAATCAAAACGTTACCTAAATCTAGCTGGACAAGCAATTTCATACAATGCTGGTAAAGAAAAGATGCTATCTCTATATGACCAAGTACGTAAACACTTAGGACTAACAAGAAGAGACTTTGTTGAAAAAGAAAACCACAAATACATAAAAGAATTCTTTGACGTTCTATTAAAGAATAGTGCCTTACCTCTAGATACTTTAGAAAAAGTTACAAAGATTCACTACGGTATAAAATAA", "aa": "MLQYFGALNEAQLRNMRRAVDPAYHGQGIEAKSDLPANASINDVRRFMSANSALGVGDIMSESKRYLNLAGQAISYNAGKEKMLSLYDQVRKHLGLTRRDFVEKENHKYIKEFFDVLLKNSALPLDTLEKVTKIHYGIK", "aa_hexdigest": "7d0c5c5299ac69a4bea75ffe07e85015", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A3B0P8H0", "query_cov": 0.9424460431654677, "subject_cov": 1.0234375, "identity": 0.603, "score": 163.0, "evalue": 1.95e-49, "valid": false, "product": "Bacterial protein of uncharacterized function (DUF885)", "uniref50_id": "UniRef50_A0A3B0P8H0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "product": "Bacterial protein of uncharacterized function (DUF885)" }, "genes": [], "id": "AHCKFKLKGB_818", "locus": "GCA013367975_00810" }, { "type": "cds", "sequence": "contig_1", "start": 472337, "stop": 472444, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACACCACAACCAAATTTACTACGCACGTAAACACCTAGCAAGTCAAAACAATGAAAATCTAGGTGCAAATGCATTTGACTTTACTTCATACGTTGAAGGTTGA", "aa": "MGHHNQIYYARKHLASQNNENLGANAFDFTSYVEG", "aa_hexdigest": "85eaea3623aa17efcf7969704c24eb3e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3998.2690000000002, "isoelectric_point": 6.217469596862792 }, "id": "AHCKFKLKGB_819", "locus": "GCA013367975_00811" }, { "type": "cds", "sequence": "contig_1", "start": 472478, "stop": 472687, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTCAACCATTAAGAATGGAAAATCATTCTTCGGTAAAGATGTTCCTGACTACAAGGCAGTAACATATCTATATGAAAGACGTGATCAAGAAGGTGTTGGAGCATATAACTCAGATACACGTATCTTAGATGCAAATGGAAACGTAACTTCTGTTCAAGGTGCATTTAACTTCAACTGTGACCCATACTACTCATTGCCAAAATGA", "aa": "MKSTIKNGKSFFGKDVPDYKAVTYLYERRDQEGVGAYNSDTRILDANGNVTSVQGAFNFNCDPYYSLPK", "aa_hexdigest": "167242c5e91e4860dfec3e3d008e0322", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7766.516000000001, "isoelectric_point": 7.807008552551269 }, "id": "AHCKFKLKGB_820", "locus": "GCA013367975_00812" }, { "type": "cds", "sequence": "contig_1", "start": 473033, "stop": 473365, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAGACAATGTTTACAATGAAACAGAAAACACAATATCAGTTTCAAAAGAAGCAGACGGAAAACTTACTGAAAGATCAGTTTATGGTTTAGGTTTTACCAAATTTGACTTAGAAGCTAAGGGCGTTGGATTCTCACAAGTTGGCGGTGATGAAACTGAAAGTGGAAAGACCATTTATGACCAAATCACAAAAATGGCAACCACATCAGAAGACACTCCAAATGATGTATATCAATCAGGATACAAAACATCAAAAGGTGTTTCAAAGAACATGACTGCTATTGCATCAGAAGTTGCTAAATTAATAGCTGGTGAAAGTGGAGAATGA", "aa": "MYKDNVYNETENTISVSKEADGKLTERSVYGLGFTKFDLEAKGVGFSQVGGDETESGKTIYDQITKMATTSEDTPNDVYQSGYKTSKGVSKNMTAIASEVAKLIAGESGE", "aa_hexdigest": "ddc9d71d2c0219ebd9ca6858eec68c31", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11840.85089999999, "isoelectric_point": 4.673495292663574 }, "id": "AHCKFKLKGB_821", "locus": "GCA013367975_00813" }, { "type": "tRNA", "sequence": "contig_1", "start": 474640, "stop": 474729, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 64.9, "nt": "GGATAGTTGCTCGAGAGGCTGAAGAGGTTAGTCTCGAAAACTAATAATGTTTTCTAACATTCAAGGGTTCAAATCCCTTACTATCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 474716, 474718 ], "id": "AHCKFKLKGB_26", "locus": "GCA013367975_00814" }, { "type": "cds", "sequence": "contig_1", "start": 474989, "stop": 476488, "strand": "+", "frame": 2, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537", "KEGG:K15633", "RefSeq:WP_175933534.1", "SO:0001217", "UniParc:UPI00158946B2", "UniRef:UniRef100_UPI00158946B2", "UniRef:UniRef50_Q8EW33", "UniRef:UniRef90_Q6Y8Q8" ], "nt": "ATGAAAAAAATAATTCTTACAATTATCGATGGACTTGGCCTAAGAAAAGAAAGGCAAGGAAATGCATATTTGCAAGCAAAACACCCTTGCTTTGACTATCTTTTTTCAATGTGCCCTAATTCTGTTTTACAAGCATCTGGTCAATATGTTGGTTTGCCAGAGGGACAAATTGGTAATAGCGAAGTAGGACATTTAAATATTGGCGCAGGTAGAGTTGTATATACGGGACTATCTTTAATTAATAAGGCAATTGAAAATAATACTTTTAAGGATAATGAAATTTTAAATGATGTCATTGATAAAACTATTAAAAATAATACAACATTGCATGTAATGGGTTTATTAAGCAACGGCGGTGTTCATTCATTAGATTTACATTTGTTTGAAATTTTAAAATTAGCGCATAGTAAAGGATTAAAAAATGTAAGTGTTCATGTGTTTGGTGATGGAAGAGATGTAAAGCCTCAATCAATAAAAAATTCTCTTGAAACCTTAAAAGATTTATGCCAAAAATTTGGATATAAAATCTCTTCTATATCTGGCAGATTTTATGCAATGGATAGAGATAGTATATTTTCAAGAAATCAAGAGGCATATGATGCCATTTTAGGACAATCAAAAAATGTTATTGAAAACATTGATGATTACATTGAAAGTCAATATAAAAAAGGAATATTTGACGAATTTTTTAAACCTGCTCAATTAACAGATGGCTCATTTGTTAAAAATGGAGATTCGATTATATTCTTTAACTTTAGACCAGACAGAGCAAGACAACTAAGCCATATGTTTATAGGTTCAAATTTATACACATACGAACCAAAAAACAAAGTACAAATCGATAATTTTGTTTCATTGATGAAATATGAAGGTATAAATAGTAAGGTCGCCTTTAAAGAAATGGAAGTTGTAAATCCACTAGGCAAGGTTTTAGAAAATAATGACATTAAACAATTAAGACTAGCAGAAACTCAAAAATATGCCCATGTAACATTCTTTTTTGATGGAGGGGTTGACATTGAATATAAAAACGAAAATAGAATTTTAGTTGATAGTCTTAAGGTTGATTCATTTGCTGATTATCCACATATGTCGGCCAAAGAAATTACAGATTCATTATTGAACAACATTGAAAAAAATGATTTTATAATAATGAATTATGCAAATCCTGATATGGTGGGTCATACCGGCAATTTAAATGCAACAATTGAAGCAATTGAATTTTTAGATTCACAATTCCAAAGAATATTAGAATATGTGTCATTAAATCATGAAAATGTAACTTGGTTTATTACAGCAGATCATGGCAATGCCGAAATTACAGAAGATGAAAATAACAAACCAGCAACAAAACATACAACAAGCCCAGTAATGTTTATTTGTACGGATAAGAATGTTAATTTAGAAAATGGTAGTCTTTGCGATGTAGCTCCTACAATATTAGATTATTTAAAAATCAATAAACCAAAAGAAATGACCGGAAAATCATTGCTTAAATAA", "aa": "MKKIILTIIDGLGLRKERQGNAYLQAKHPCFDYLFSMCPNSVLQASGQYVGLPEGQIGNSEVGHLNIGAGRVVYTGLSLINKAIENNTFKDNEILNDVIDKTIKNNTTLHVMGLLSNGGVHSLDLHLFEILKLAHSKGLKNVSVHVFGDGRDVKPQSIKNSLETLKDLCQKFGYKISSISGRFYAMDRDSIFSRNQEAYDAILGQSKNVIENIDDYIESQYKKGIFDEFFKPAQLTDGSFVKNGDSIIFFNFRPDRARQLSHMFIGSNLYTYEPKNKVQIDNFVSLMKYEGINSKVAFKEMEVVNPLGKVLENNDIKQLRLAETQKYAHVTFFFDGGVDIEYKNENRILVDSLKVDSFADYPHMSAKEITDSLLNNIEKNDFIIMNYANPDMVGHTGNLNATIEAIEFLDSQFQRILEYVSLNHENVTWFITADHGNAEITEDENNKPATKHTTSPVMFICTDKNVNLENGSLCDVAPTILDYLKINKPKEMTGKSLLK", "aa_hexdigest": "39c94369d22a024298cce8ab8b088e9c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158946B2", "ncbi_nrp_id": "WP_175933534.1", "uniref100_id": "UniRef100_UPI00158946B2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158946B2", "RefSeq:WP_175933534.1", "UniRef:UniRef100_UPI00158946B2" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158946B2", "uniref90_id": "UniRef90_Q6Y8Q8" }, "psc": { "uniref90_id": "UniRef90_Q6Y8Q8", "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_Q8EW33", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EW33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EW33" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "AHCKFKLKGB_822", "locus": "GCA013367975_00815" }, { "type": "cds", "sequence": "contig_1", "start": 476531, "stop": 477019, "strand": "-", "frame": 3, "gene": null, "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZZ8" ], "nt": "TTGCAAGATACATTTCTATTTAAAACAACAATTTTAGAAAATCTAAGATATGCTAATCCTAGTGCAAGCAATGAAGAAATTATTAATGCTGCCAAAATTACAAAGGCTGATAATTTTATAAGACAACTTGAACACGGTTACGAAGAAATAGTTGAAGAAGGCGGAAGCAATTTTTCACAAGGTGAAAGGCAAATGCTTGCAATCACAAGAGCAATAATTGCTAATAAAAATATTTTGATATTGGATGAAGCAACATCTAATGTAGATACAAGAACAGAAAAGAATATTCAAGATGCAATGTTAGAGTTAATGAAAGGAAAAACTTCATTTGTTATTGCTCATAGACTTTCAACAATAGTAAATGCTAACCAAATTTTAGTTGTTAAAGATGGAAAAATTATTGAACAAGGTACACACAAAGAACTATTGGCCAAAGGTAGTTTTTATGAAAAGCTATATCATTCATCTTTTAGTGAAGAAAATGAATAA", "aa": "MQDTFLFKTTILENLRYANPSASNEEIINAAKITKADNFIRQLEHGYEEIVEEGGSNFSQGERQMLAITRAIIANKNILILDEATSNVDTRTEKNIQDAMLELMKGKTSFVIAHRLSTIVNANQILVVKDGKIIEQGTHKELLAKGSFYEKLYHSSFSEENE", "aa_hexdigest": "4741d9406dc7d1a1a61753d7e98d8d00", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NZZ8", "query_cov": 0.9567901234567902, "subject_cov": 1.0, "identity": 0.742, "score": 231.0, "evalue": 9.17e-76, "valid": false, "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component", "uniref50_id": "UniRef50_A0A3B0NZZ8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NZZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZZ8" ], "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component" }, "genes": [], "id": "AHCKFKLKGB_823", "locus": "GCA013367975_00816" }, { "type": "cds", "sequence": "contig_1", "start": 477041, "stop": 478102, "strand": "-", "frame": 3, "gene": "mdlB", "product": "ABC transporter ATP binding protein", "db_xrefs": [ "COG:COG1132", "COG:V", "SO:0001217", "UniRef:UniRef50_A0A448ZXF5", "UniRef:UniRef90_D1J8H0" ], "nt": "TTGATCTTAGGCTATATTTTAGAAAAAGTATTTTCATTTATATGTTCAGCAATGGCAGTCAAGGCAGGAATTTATGGTTCAGAAATAATGCGGAGTAAGGCTTATAAATCAATAATGAAAATGCCAATCCAATTTTTTGATACTGTTAGCTCAGGAGAATTAATGTCAATATTGTCAAATGATATTGATAATGTTTCATATGGAATATGTGGAACTTTAAACTCGTTATTAGCAGCGATATTTAGTGCTTTAACATCGCTTGGATTTATGTTTTACTATTCTACATATTTAACACTTATATATATTGCCTTATTTCCTGTTTTGTTGATATTGGCCTTTGTTTTAATCAAAAAAGCAATTCCATTATTTGGAAAGACTCAAAGAAATATAGGTTTATTGAATGGTTTTGTTGAAGAAAATTTAGCAGCACATCAATTAATAAATTCATTGAATAGTCAACAAATTATTAACGAACAATTTGATTTATATAATGAAAAATTATATAAACCTTCATTGAAAGCAAATATATATGCCGGAATAGTTTATCCATATGGAAGTGCAATAACATTCATAATGCAATTAATAATAATTTTTGTTGGCGGAGCATTTGCATTGTCTGGAATTGGCACTGGCTCAAATAGTAATTTTACAGTGGGTACATTGACATCATTTATTTTGTATATAAAAATTACTTCTAGTCAGATAATTAAAAATTTTGAAAATATTTCAATGATTCAAACAGCAACAATATCAACAACAAGAGTATTAAATTTAATTAGAATGCATCCGGCCGTTGATGAAAGCAAACTTGCTACAATATCAAATGTGAATGGTGATATTGAATTTAAAAATGTAAGTTTTTCATATGATAATTCTTTGAATAATTTACAATTGAAAAATATTTCATTCAAAGCAAAAAAAGGTCAAGTGTTTGCTATTGTAGGACCTACCGGGGCAGGCAAAACTACAATAATAAATTTACTAAGCAAATTTTATTTGCCTATTGAAGGAGAAATAAAAATTGATGATTTTAAATCAAGTGAAATAAATGAATCTAGTTGA", "aa": "MILGYILEKVFSFICSAMAVKAGIYGSEIMRSKAYKSIMKMPIQFFDTVSSGELMSILSNDIDNVSYGICGTLNSLLAAIFSALTSLGFMFYYSTYLTLIYIALFPVLLILAFVLIKKAIPLFGKTQRNIGLLNGFVEENLAAHQLINSLNSQQIINEQFDLYNEKLYKPSLKANIYAGIVYPYGSAITFIMQLIIIFVGGAFALSGIGTGSNSNFTVGTLTSFILYIKITSSQIIKNFENISMIQTATISTTRVLNLIRMHPAVDESKLATISNVNGDIEFKNVSFSYDNSLNNLQLKNISFKAKKGQVFAIVGPTGAGKTTIINLLSKFYLPIEGEIKIDDFKSSEINESS", "aa_hexdigest": "79209757c73d07ec001e58f173e41a17", "start_type": "TTG", "rbs_motif": null, "genes": [ "mdlB" ], "truncated": "both", "pseudogene": { "start": 476738, "stop": 478402, "observations": { "mutation": [ 477043, 478153 ], "stop": [ 477043, 478153 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H0", "query_cov": 1.0, "subject_cov": 0.5648, "identity": 0.9890000000000001, "score": 650.0, "evalue": 1.45e-230, "gene_start": 1, "gene_end": 353, "reference_start": 103, "reference_end": 455, "reference_sequence": "MNRDILDPLKVDIDLKKVSKFERKAFRKKQHAIKSESFKELIKYLNYKRGYVFLIIIITLISSLCLTAATYGLGYIIDHYLNFKFWNSNSNGLFEFGIAIGILILGYILEKVFSFICSAMAVKAGIYGSEIMRSKAYKSIMKMPIQFFDTVSSGELMSILSNDIDNVSYGICGTLNSLLAAIFSALTSLGFMFYYSTYLTLIYIALFPVLLILAFVFIKKAIPLFGKTQRNIGLLNGFVEENLAAHQLINSLNSQQIINEQFDLYNEKLYKPSLKANIYAGIVYPYGNAITFIMQLIIIFVGGAFALSGIGTGSNSNFTVGTLTSFILYIKITSSQIIKNFENISMIQTATISTTRVLNLIRMHPAVDESKLATISNVNGDIEFKNVSFSYDNSLNNLQLKNISFKAKKGQVFAIVGPTGAGKTTIINLLSKFYLPIEGEIKIDDYKSSEINESSWRNQISIVLQDTFLFKTTILENLRYANPSASNEEIINAAKITKADNFIRQLEHGYEEIVEEGGSNFSQGERQMLAITRAIIANKNILILDEATSNVDTRTEKNIQDAMLELMKGKTSFVIAHRLSTIVNASQILVVKDGKIIEQGTHKELLTKGSFYEKLYHSSFSEENE" }, "paralog": false, "identity": 0.9909747292418772, "score": 1053.0, "evalue": 0.0, "description": "Internal stop codon at 477043, 478153. Nonsense mutation around 477043, 478153.", "psc": { "uniref90_id": "UniRef90_D1J8H0", "gene": "MdlB", "product": "ABC transporter ATP binding protein", "uniref50_id": "UniRef50_A0A448ZXF5", "cog_id": "COG1132", "cog_category": "V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H0", "UniRef:UniRef50_A0A448ZXF5", "COG:COG1132", "COG:V" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXF5" ], "product": "ABC-type multidrug/protein/lipid transport system ATPase component" } }, "id": "AHCKFKLKGB_824", "locus": "GCA013367975_00817" }, { "type": "cds", "sequence": "contig_1", "start": 478151, "stop": 478408, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGGGATATTCTTGATCCACTAAAAGTTGATATTGATTTAAAGAAAGTATCTAAATTTGAAAGAAAAGCATTTCGAAAAAAACAACACGCAATAAAATCAGAAAGTTTTAAAGAACTAATTAAATATTTAAATTACAAACGTGGATATGTTTTTTTAATAATAATAATTACATTAATTTCTTCATTGTGTTTAACTGCCGCCACATACGGTTTGGGCTATATTATTGACCATTATTTAAACTTTAAGTTTTGA", "aa": "MNRDILDPLKVDIDLKKVSKFERKAFRKKQHAIKSESFKELIKYLNYKRGYVFLIIIITLISSLCLTAATYGLGYIIDHYLNFKF", "aa_hexdigest": "a80f3f94ab1456aec97f2b99dee99346", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10062.88029999999, "isoelectric_point": 9.714434623718262 }, "id": "AHCKFKLKGB_825", "locus": "GCA013367975_00818" }, { "type": "cds", "sequence": "contig_1", "start": 478401, "stop": 479303, "strand": "-", "frame": 2, "gene": null, "product": "Multidrug ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D3WBZ0" ], "nt": "ATGAATTACGTTATGACTGTTACATTTGGAGTTGTTGCTTCATCATTTACTACATTTAATATTTTTAAAGCAAATGTATCCTCAAAACGTATTTTTGAAATTATGAACAAAAAGCCAGATATTGCAAAAATCAAATCTGATAAGCTAATTGTTAATGGAGAAATTGAATTTAGCCATGTATCTTTTAAATATTATGAATCTGCCAAATCTAATGTTTTAGAAGATATTTCATTTACTTTAAAGCCTGGTAAGGTTTTGGGTATAATTGGCCCTACTGGTTCTGGAAAATCAACTATTGCAAAATTATTAAACTTAGATTTTAAAACTCAAGAAGGGCTAGTAACAATTGACGGGCATAATATTCAAGAAATTGATACAGATTCATTGAGAAAGAATATCTCCCACGTTTATCAAAACCCTTGCCTATTAAGTGGCACAATAAAAAGCAATTTATTGCTTGCTAAACCTAATGCCACTGATGAAGAGATTGAATTAGCAACAAAAAATGGTTGTGCCTTTGAATATATCAATAAGTTTAAAGAAAAATTTGAACATGTTGTTGAACAAAAGGGCGCAAATTTATCAGGCGGTCAAAAACAAAGATTATCTATCGCCCAAGGTTTGATCAAACGACCTAAAATATTGATTTTAGATGATTCTACTTCGGCATTGGATGCTAGAACAGAAGCAATGGTAAGAAACAATATTAAAGAAGAATTTAAATACGAAAAAATATCATTAGTTATTATTGCCCAAAAAATTTCAGCAATTATTGATGCAGATGAAATTTTAGTATTAAACCAAGGAAAAATTATTGATCGTGGCAATCATGAGGAATTAATTTCTAGAGATGGTCTATATAAAGAAATTGCATATTCACAGTTAGGGGGCAATAATGAATAG", "aa": "MNYVMTVTFGVVASSFTTFNIFKANVSSKRIFEIMNKKPDIAKIKSDKLIVNGEIEFSHVSFKYYESAKSNVLEDISFTLKPGKVLGIIGPTGSGKSTIAKLLNLDFKTQEGLVTIDGHNIQEIDTDSLRKNISHVYQNPCLLSGTIKSNLLLAKPNATDEEIELATKNGCAFEYINKFKEKFEHVVEQKGANLSGGQKQRLSIAQGLIKRPKILILDDSTSALDARTEAMVRNNIKEEFKYEKISLVIIAQKISAIIDADEILVLNQGKIIDRGNHEELISRDGLYKEIAYSQLGGNNE", "aa_hexdigest": "26ef070b6fb59b16098af51e462c02da", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031CC58F0", "query_cov": 0.8, "subject_cov": 0.9876543209876543, "identity": 0.5, "score": 214.0, "evalue": 2.32e-65, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A3D3WBZ0" }, "pscc": { "uniref50_id": "UniRef50_A0A3D3WBZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D3WBZ0" ], "product": "Multidrug ABC transporter ATP-binding protein" }, "genes": [], "id": "AHCKFKLKGB_826", "locus": "GCA013367975_00819" }, { "type": "cds", "sequence": "contig_1", "start": 479865, "stop": 480224, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter transmembrane domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241122C8", "UniRef:UniRef90_UPI00241122C8" ], "nt": "ATGTTTAAACTTTATAAATTAATGGCCAACAAATTCAAATGTTTGTCATTTTTAACTATATCTTTAACATTGCTACAAGTAGTTTCATTTTTAGTATTGCCAATATTATTGGGACAATTGACAAGGTTAATTGGTGAAAATGCATATTTAATTCAAAATAATTTGTCAACAAACAGACCAATAACAATTGAAATTCTAAGAATTAATTTTTTATGTCAAAGCCACCAGAGTGCATTAATGCATTTGGGGGGATATTTTGCATTGTTTTTAATAATAGGAACTATTTCGGCAATGTGTGCTTCATTGCTTGCATCATATGTGTCTCAAGCAGGATCAAAACAAATAAGAAGTTGTTTATGA", "aa": "MFKLYKLMANKFKCLSFLTISLTLLQVVSFLVLPILLGQLTRLIGENAYLIQNNLSTNRPITIEILRINFLCQSHQSALMHLGGYFALFLIIGTISAMCASLLASYVSQAGSKQIRSCL", "aa_hexdigest": "3356d1856d119451e55003d5e2b1adf3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 479564, "stop": 480224, "observations": { "insertion": [ 479600 ], "mutation": [ 479615, 479867 ], "stop": [ 479615, 479867 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00241122C8", "query_cov": 1.0, "subject_cov": 0.5265486725663717, "identity": 1.0, "score": 216.0, "evalue": 1.44e-69, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MFKLYKLMANKFKCLSFLTISLTLLQVVSFLVLPILLGQLTRLIGENAYLIQNNLSTNRPITIEILRINFLCQSHQSALMHLGGYFALFLIIGTISAMCASLLASYVSQAGSKQIRSCLWKHLGELSQKDIEAFSNAKILTRFTIDISRIQTGLMSFLRMLIIGPFNLVLGLVFALLTNLQLSMIFLVVIPLLTLTMVISGVIWNPIQKKNKKCMTKLTLKVEKIF" }, "paralog": false, "identity": 0.9908675799086758, "score": 405.0, "evalue": 1.88e-146, "description": "Internal stop codon at 479615, 479867. Frameshift due to insertion around 479600. Nonsense mutation around 479615, 479867.", "psc": { "uniref90_id": "UniRef90_UPI00241122C8", "product": "ABC transporter transmembrane domain-containing protein", "uniref50_id": "UniRef50_UPI00241122C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00241122C8", "UniRef:UniRef50_UPI00241122C8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00241122C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241122C8" ], "product": "ABC transporter transmembrane domain-containing protein" } }, "id": "AHCKFKLKGB_827", "locus": "GCA013367975_00820" }, { "type": "cds", "sequence": "contig_1", "start": 480273, "stop": 480686, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATGAAGAATCAGGATTATTAGGAGTTTCAAAAATTTCTTCTGATATTCGTGACGTTGAAGAAGCTAAAAAAACAAATGAAAGAGCAAAATTTGCAATAGATTTATATGTAAGAAAAATAGCAGATTATTTAATTAATTACATGAATAAGATTGAAGGAAAAATCGATGCTATTGTTTTTACGGCGGGTGTGGGTGAAAATGATGAATATATTCGCCAAGCAGTTGTTGATAAAATTCATTTAGTTAAATTAGAAATTGATAGCGAACTAAATAAAGGAAGAGATTTTGATTCATACAAATTAATTTCAACTAAAAATTCTTCAATTCCTATTTATATAATAAGGGCAGATGAAGAACTTTATATCGCAAAAGAAGTTAAACAATTTTTTGATGATAATACAAGGTAA", "aa": "MLNEESGLLGVSKISSDIRDVEEAKKTNERAKFAIDLYVRKIADYLINYMNKIEGKIDAIVFTAGVGENDEYIRQAVVDKIHLVKLEIDSELNKGRDFDSYKLISTKNSSIPIYIIRADEELYIAKEVKQFFDDNTR", "aa_hexdigest": "0eeeaf365f428ed7272c04885b361866", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15741.707099999985, "isoelectric_point": 5.012255668640137 }, "id": "AHCKFKLKGB_828", "locus": "GCA013367975_00821" }, { "type": "cds", "sequence": "contig_1", "start": 480708, "stop": 481052, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTCCACTTTGATACATCATTTCATAAAACCCTTCCAAAAGAAGCATATACTTATGCAATTGATGCAAATATAACCGATGAATTAAATGTTAGAAAATATGGTTTCCATGGCTTGAATCACCACTTTATTACATTAAAATTGCAAGAAATTTTAAATAAAGATAAAGTTAATTTTGTTAATTTACATATCGGAAATGGCGCAAGTTTAGCAGCCATTCAAGATAGTAAATCAATTGACACTTCAATGGGATTTACACCATTGGCAGGGGTTATGATGGGAACTCGTTCTGGTGATGTTGATTGTTCAATTATTCCATATATTATGAAACATAAAAATTGA", "aa": "MTFHFDTSFHKTLPKEAYTYAIDANITDELNVRKYGFHGLNHHFITLKLQEILNKDKVNFVNLHIGNGASLAAIQDSKSIDTSMGFTPLAGVMMGTRSGDVDCSIIPYIMKHKN", "aa_hexdigest": "bf9ea4f0f79cb2cb311df2ef9af91b04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12773.492099999985, "isoelectric_point": 7.055731010437011 }, "id": "AHCKFKLKGB_829", "locus": "GCA013367975_00822" }, { "type": "rRNA", "sequence": "contig_1", "start": 481557, "stop": 481661, "strand": "-", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8823529411764706, "score": 60.7, "evalue": 1.1e-09, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "CGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCCTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTT", "id": "AHCKFKLKGB_37", "locus": "GCA013367975_00823" }, { "type": "cds", "sequence": "contig_1", "start": 481764, "stop": 481961, "strand": "-", "frame": 2, "gene": null, "product": "Smr domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN26", "UniRef:UniRef90_D1J8H3" ], "nt": "ATGAAGTTCAATTTTTTTCCAATGGTGTTAGATCTTCATGGCTATGACGTAAATTCCGCAACAGCAGCAATTTTAAATGCTTTATATGAATTTGAACAAGATGAATATAATAATTATTTCGATATTATTGTAGGCATTGGTAGTGGAATTATTCGCCAAATAACGGAAGACTTATTAGACAAAGAGGGATATTATTGA", "aa": "MKFNFFPMVLDLHGYDVNSATAAILNALYEFEQDEYNNYFDIIVGIGSGIIRQITEDLLDKEGYY", "aa_hexdigest": "2a385e12833b9e3f34a5c0e1508920da", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 481716, "stop": 481961, "observations": { "mutation": [ 481766 ], "stop": [ 481766 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H3", "query_cov": 1.0, "subject_cov": 0.7926829268292683, "identity": 1.0, "score": 136.0, "evalue": 7.85e-41, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MKFNFFPMVLDLHGYDVNSATAAILNALYEFEQDEYNNYFDIIVGIGSGIIRQITEDLLDKEGYYWKNIGNSLAKIRVFKKI" }, "paralog": false, "identity": 0.9876543209876543, "score": 162.0, "evalue": 1.41e-53, "description": "Internal stop codon at 481766. Nonsense mutation around 481766.", "psc": { "uniref90_id": "UniRef90_D1J8H3", "product": "Smr domain-containing protein", "uniref50_id": "UniRef50_B3PN26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H3", "UniRef:UniRef50_B3PN26" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN26" ], "product": "Smr domain-containing protein" } }, "id": "AHCKFKLKGB_830", "locus": "GCA013367975_00824" }, { "type": "cds", "sequence": "contig_1", "start": 481962, "stop": 482789, "strand": "-", "frame": 2, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "EC:3.2.2.23", "EC:4.2.99.18", "GO:0005737", "GO:0006281", "GO:0008534", "GO:0046872", "KEGG:K10563", "RefSeq:WP_049342447.1", "SO:0001217", "UniParc:UPI00066DC576", "UniRef:UniRef100_UPI00066DC576", "UniRef:UniRef50_A0A9Q9F2U2", "UniRef:UniRef90_A0A454C9Y4" ], "nt": "ATGCCTGAATTACCTGAAGTTAGGGTTGTTGTTAAAGCCCTTAATAATACTATATTAAATAAAAAAATAACAAATTTGATTATATACAAACCCAAAATTTTTAAAGAGTTTAGCCCTCAATATTTCATTTCAATTTTAAGAGATAAAAAAATTGAAAAAATAGATAACATTGGCAAACATATAATTTTCTTTTTATCTGAAAAATTAGTTTTACTTTCTCATTTAAGAATGGAAGGAAAATATCGTTATTATTTAAATCCACCCAAAGAAATTGATAAGCACTTAGTTGCTAGATTTGTTTTTAGCGACAACAGTGAACTACACTTCTTAGATAAACGTTTATTTGGTACATATATGCTAAGAAACTTAGAAAATTATAACAAAATTCCTCCAATTTCTTTGCTTGGACCTGAACCAAAGGATATTGACATTGAAGCATTATTTAAAAAAATAAAAAATTCAAAAATGCCCATTAAAACAAAATTACTTGATCAAAGTTTTGTTGCGGGTATAGGAAATATTTACGCTGATGAAGCATTGTTTGCTTCCAAAATTCATCCTCTTTCAAAAGCTTCAAATTTATCACTAGAGCAACTTAAAGACATAATAAAAAACGCAAATGAAATAATGGAAAGAAGTTATGAGCTAGGTGGAACAACGTTATTTTCTTATGAATCATTAAATAAACAAGAAGGAAAATATCAAGATTTTTTAAATATTCATAGTGATAATATAAAAAAATGTAAGGTTTGTGGCCAAAAAACACTTAAATTAAAAGTTAACCAACGTGGCACATATGTTTGTCCTAATTGTCAGAAGGTATATTAA", "aa": "MPELPEVRVVVKALNNTILNKKITNLIIYKPKIFKEFSPQYFISILRDKKIEKIDNIGKHIIFFLSEKLVLLSHLRMEGKYRYYLNPPKEIDKHLVARFVFSDNSELHFLDKRLFGTYMLRNLENYNKIPPISLLGPEPKDIDIEALFKKIKNSKMPIKTKLLDQSFVAGIGNIYADEALFASKIHPLSKASNLSLEQLKDIIKNANEIMERSYELGGTTLFSYESLNKQEGKYQDFLNIHSDNIKKCKVCGQKTLKLKVNQRGTYVCPNCQKVY", "aa_hexdigest": "2a62468c705066e96a9a534bc9a85185", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00066DC576", "ncbi_nrp_id": "WP_049342447.1", "uniref100_id": "UniRef100_UPI00066DC576", "db_xrefs": [ "SO:0001217", "UniParc:UPI00066DC576", "RefSeq:WP_049342447.1", "UniRef:UniRef100_UPI00066DC576" ] }, "ips": { "uniref100_id": "UniRef100_UPI00066DC576", "uniref90_id": "UniRef90_A0A454C9Y4" }, "psc": { "uniref90_id": "UniRef90_A0A454C9Y4", "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23", "4.2.99.18" ], "uniref50_id": "UniRef50_A0A9Q9F2U2", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0005737", "GO:0006281", "GO:0008534", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2U2" ], "product": "DNA-(apurinic or apyrimidinic site) lyase" }, "genes": [ "mutM" ], "id": "AHCKFKLKGB_831", "locus": "GCA013367975_00825" }, { "type": "cds", "sequence": "contig_1", "start": 482866, "stop": 483042, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAATAAATATCAAAAATTAATTATTTCAACTACAGACACAGTTTTAGGAATTGGTGGCCCTGTGTCAAAAGAAGTTGAAGACCTTATTTATGAAATAAAAGGTAGAGACCGCAATAAAAAATTAATAATATTAGTAAGTTCAATAAAACAAGCAAGGCAATTTAAGGAATGA", "aa": "MINKYQKLIISTTDTVLGIGGPVSKEVEDLIYEIKGRDRNKKLIILVSSIKQARQFKE", "aa_hexdigest": "fffbfe4be100210b6dc72de2222d76bc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6619.7276, "isoelectric_point": 9.629852104187012 }, "id": "AHCKFKLKGB_832", "locus": "GCA013367975_00826" }, { "type": "cds", "sequence": "contig_1", "start": 483121, "stop": 483333, "strand": "+", "frame": 1, "gene": "tsaC", "product": "L-threonylcarbamoyladenylate synthase", "db_xrefs": [ "COG:COG0009", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A077L684", "UniRef:UniRef90_D1J8H5" ], "nt": "ATGCCAAACCAAAAGAAATTGCTTGAATATATCGAAATAAATGGTCCAATTTATATGACAAGTTGTAACTTGTCAAACGCACCTGTATGTAAAACTATTGAAAATGCAAAGCAATTATTCCCTGAAGTTACTAATGTATATAATTTTGGCCCTATGACTCAAAAACCTAGTCAAATAATTAGAGTAGAAGATGGAGAAATTTTAAGAAAATAA", "aa": "MPNQKKLLEYIEINGPIYMTSCNLSNAPVCKTIENAKQLFPEVTNVYNFGPMTQKPSQIIRVEDGEILRK", "aa_hexdigest": "05a65d3a945978a0650e82ea69fd695c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "tsaC" ], "truncated": "5-prime", "pseudogene": { "start": 482866, "stop": 483333, "observations": { "mutation": [ 483040, 483076 ], "stop": [ 483040, 483076 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H5", "query_cov": 1.0, "subject_cov": 0.45161290322580644, "identity": 0.986, "score": 146.0, "evalue": 9.69e-44, "gene_start": 1, "gene_end": 70, "reference_start": 86, "reference_end": 155, "reference_sequence": "MINKYQKLIISTTDTVLGIGGPVSKEVEDLIYEIKGRDRNKKLIILVSSIKQARQFKEWNSQADEFAKKYWPGATTIVVNNQGFRMPNQKKLLEYIEVNGPIYMTSCNLSNAPVCKTIENAKQLFPEVTNVYNFGPMTQKPSQIIRVEDGEILRK" }, "paralog": false, "identity": 0.9806451612903225, "score": 301.0, "evalue": 2.09e-107, "description": "Internal stop codon at 483040, 483076. Nonsense mutation around 483040, 483076.", "psc": { "uniref90_id": "UniRef90_D1J8H5", "gene": "TsaC", "product": "L-threonylcarbamoyladenylate synthase", "uniref50_id": "UniRef50_A0A077L684", "cog_id": "COG0009", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H5", "UniRef:UniRef50_A0A077L684", "COG:COG0009", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L684", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L684" ], "product": "L-threonylcarbamoyladenylate synthase" } }, "id": "AHCKFKLKGB_833", "locus": "GCA013367975_00827" }, { "type": "cds", "sequence": "contig_1", "start": 483335, "stop": 483730, "strand": "+", "frame": 2, "gene": "rpiB", "product": "Ribose-5-phosphate isomerase", "db_xrefs": [ "COG:COG0698", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J8H6", "UniRef:UniRef90_D1J8H6" ], "nt": "ATGAAAACGACAGTTAAAATAACAAGCGATCACGCAGGATATGATGCAAAAATAAACCTTGCAAAAAGATTGGAAAATGAAGGCTACAATGTAGAATTATTTGGCTCAAAAACAAATAACAATAGTGATTCTTATGCAGATGCTGCTATTGAATTAGTTAATGAAATTCACAAGGATGGCAATCCTGATGAAAATAAAAATGTAAAATATGTTGCATTTTGTGGATCAGGTATTGGTATTGAAATGACTTTAAACAAATTTAAAGGCATAAGAGCAGCAAGAGTTACAAATGAAAACGAATCTCACCTAGCAAAATTGCATAATAATGCTAACGTACTATGTATGGGTGGAAGATTGTTAGAAGGTAATGAAATTGAAAAAATTTTTCATAATTGA", "aa": "MKTTVKITSDHAGYDAKINLAKRLENEGYNVELFGSKTNNNSDSYADAAIELVNEIHKDGNPDENKNVKYVAFCGSGIGIEMTLNKFKGIRAARVTNENESHLAKLHNNANVLCMGGRLLEGNEIEKIFHN", "aa_hexdigest": "904795d05bfcdd10308dac323e4c9276", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8H6", "query_cov": 1.0, "subject_cov": 0.8451612903225807, "identity": 0.985, "score": 263.0, "evalue": 1.18e-88, "valid": true, "gene": "RpiB", "product": "Ribose-5-phosphate isomerase", "uniref50_id": "UniRef50_D1J8H6", "cog_id": "COG0698", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_D1J8H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8H6" ], "product": "Ribose-5-phosphate isomerase" }, "genes": [ "rpiB" ], "id": "AHCKFKLKGB_834", "locus": "GCA013367975_00828" }, { "type": "cds", "sequence": "contig_1", "start": 484859, "stop": 485047, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00036493F6", "UniRef:UniRef90_UPI00036493F6" ], "nt": "ATGGTCATGATTATTATTTCAGCCGTTTTATACAATATTGGTGTTGCAACATTCTTGGCTAAGGCTGCCACTGTTGCAACCGGGTTATCTGCATTGGTTCAAGCCATTACTCTAACATTAACTCAAACTGCACCATATTTTGCTTATATTTATATAGCAATGAATTTACCTTTTGTAATTGCATTTTGA", "aa": "MVMIIISAVLYNIGVATFLAKAATVATGLSALVQAITLTLTQTAPYFAYIYIAMNLPFVIAF", "aa_hexdigest": "e9ecd32b7bb53b225037a9f72ff45509", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 484828, "stop": 485197, "observations": { "insertion": [ 484855 ], "mutation": [ 484861, 485068 ], "stop": [ 484836, 484861, 485068 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00036493F6", "query_cov": 1.0, "subject_cov": 0.5081967213114754, "identity": 1.0, "score": 103.0, "evalue": 2.01e-27, "gene_start": 1, "gene_end": 62, "reference_start": 51, "reference_end": 112, "reference_sequence": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNIWKKFPKKMVMIIISAVLYNIGVATFLAKAATVATGLSALVQAITLTLTQTAPYFAYIYIAMNLPFVIAFWKKILDYLWY" }, "paralog": false, "identity": 0.9754098360655737, "score": 221.0, "evalue": 3e-76, "description": "Internal stop codon at 484836, 484861, 485068. Frameshift due to insertion around 484855. Nonsense mutation around 484861, 485068.", "psc": { "uniref90_id": "UniRef90_UPI00036493F6", "uniref50_id": "UniRef50_UPI00036493F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00036493F6", "UniRef:UniRef50_UPI00036493F6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00036493F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00036493F6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6600.926899999999, "isoelectric_point": 8.138311576843265 }, "id": "AHCKFKLKGB_835", "locus": "GCA013367975_00829" }, { "type": "cds", "sequence": "contig_1", "start": 485066, "stop": 485197, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410BF5B", "UniRef:UniRef90_UPI002410BF5B" ], "nt": "ATGAAACATACAGCTGAGGAACTTGCTGAGATTAAAGAACGTAAACGTTTAAAGAAAGAAACCAAAAAAGAAAAGGGATCAGTTGGCAAAATTGACATAAATCCTTATGGGGTAAATTTCTTTAATATTTGA", "aa": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNI", "aa_hexdigest": "3bbac7b9d353dab09e5c0c372e3c1fba", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 484986, "stop": 485197, "observations": { "deletion": [ 485050 ], "mutation": [ 485068 ], "stop": [ 485068 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410BF5B", "query_cov": 1.0, "subject_cov": 0.6142857142857143, "identity": 1.0, "score": 84.0, "evalue": 1.76e-20, "gene_start": 1, "gene_end": 43, "reference_start": 1, "reference_end": 43, "reference_sequence": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNIWKKFPKKNGHDYYFSRFIQYWCCNILG" }, "paralog": false, "identity": 0.9857142857142858, "score": 143.0, "evalue": 1.79e-46, "description": "Internal stop codon at 485068. Frameshift due to deletion around 485050. Nonsense mutation around 485068.", "psc": { "uniref90_id": "UniRef90_UPI002410BF5B", "uniref50_id": "UniRef50_UPI002410BF5B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410BF5B", "UniRef:UniRef50_UPI002410BF5B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410BF5B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410BF5B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5018.7903000000015, "isoelectric_point": 9.630819129943848 }, "id": "AHCKFKLKGB_836", "locus": "GCA013367975_00830" }, { "type": "cds", "sequence": "contig_1", "start": 485364, "stop": 485633, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTACTGCAATTTATTTATTAGTATTTTCAATGTTAGTAAATAAGTTCTATCCAAAATACAAGAAAGTAAGAATTGATATTTACACCACAAAACCAGAACTAGTTGCTGAAAGATTCAAACAAATTGGATATGTTCACTCATTTAATGTATTTAACGGATTTGGTGGATATAACCATCAAGATTTTGGAAAAATTGAAACAATTGCCCTATATTTAGAAAAAGAATCAATTTTAGAAGAAGTTAGAAGTGTTGATAAAAAAGCCTGA", "aa": "MGTAIYLLVFSMLVNKFYPKYKKVRIDIYTTKPELVAERFKQIGYVHSFNVFNGFGGYNHQDFGKIETIALYLEKESILEEVRSVDKKA", "aa_hexdigest": "5fb240ebea4d8a7c69636658fba4330d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10389.952999999994, "isoelectric_point": 9.12616062164307 }, "id": "AHCKFKLKGB_837", "locus": "GCA013367975_00831" }, { "type": "cds", "sequence": "contig_1", "start": 485677, "stop": 485790, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCTTGTATATTCTAATGAACCTATTACAATTGTTGAACATGATGAAAAGCAAATTGCAACCATTAAAAATACTGTTCCTACTGGTTTTTTATTTTTATATGCAATGTAA", "aa": "MFLVYSNEPITIVEHDEKQIATIKNTVPTGFLFLYAM", "aa_hexdigest": "6e32a3089df65f17df82d5b5fbaeff0a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4274.9494, "isoelectric_point": 4.830996131896971 }, "id": "AHCKFKLKGB_838", "locus": "GCA013367975_00832" }, { "type": "cds", "sequence": "contig_1", "start": 485895, "stop": 486101, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAGAGATAATTTAGCTATTGCCAAGTATCTAGCCAACTTATTTAAAACTAACGATTTAAAAATGGTATTTGATAACGCAAAATTAATTTTTGAAAAAACTAATTCTTTATCGCTTGCTAATATCCCTAGTTTATTAACAGGTATTGATTTAAAAGGCGGTCAAAATACTAAAATTTATGGAATATATAATGGCCAAAATTGA", "aa": "MSRDNLAIAKYLANLFKTNDLKMVFDNAKLIFEKTNSLSLANIPSLLTGIDLKGGQNTKIYGIYNGQN", "aa_hexdigest": "405f761b988380877d1f43fd3ac8adfa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7537.624099999998, "isoelectric_point": 9.398346138000488 }, "id": "AHCKFKLKGB_839", "locus": "GCA013367975_00833" }, { "type": "cds", "sequence": "contig_1", "start": 486411, "stop": 486590, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGTAAGTGCATTTTTATATAATATTGCAATTACAGTCTTTTTAAAAAAAGCAGCAACAATAGCTTCAGGAACTTCATCTTTATCTCAAATAATTACTTTTACAGCGCCTGCAACTGCACAATTTTATGGTTTATTTTATGTATTAGTTAACTTACCTTTAATGTTTATTTATTGA", "aa": "MLVSAFLYNIAITVFLKKAATIASGTSSLSQIITFTAPATAQFYGLFYVLVNLPLMFIY", "aa_hexdigest": "1bf202222e35b1ddb3f6d176f53dd0d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6456.647599999998, "isoelectric_point": 9.303577613830566 }, "id": "AHCKFKLKGB_840", "locus": "GCA013367975_00834" }, { "type": "cds", "sequence": "contig_1", "start": 486618, "stop": 486746, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTAGAAGATAAAAAAATAACTAAAATCAACAAAATAATTCTTACTAAAGAGCAAAAACACCAACGGTATTTAGAAAAAATGAAAATTCTAAATCCACAAAAAATAAATTTAATAAATGTCTGA", "aa": "MSLEDKKITKINKIILTKEQKHQRYLEKMKILNPQKINLINV", "aa_hexdigest": "1873dbb81ad7f096745ecd14bcf5105a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5077.1061, "isoelectric_point": 9.998482322692869 }, "id": "AHCKFKLKGB_841", "locus": "GCA013367975_00835" }, { "type": "cds", "sequence": "contig_1", "start": 486786, "stop": 487319, "strand": "-", "frame": 2, "gene": null, "product": "Replicative DNA helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A6S1" ], "nt": "ATGTATTTAAATGATAAACCTACATCAACAATTTCGGATATAACAACCATTGCAAAACGATTTGCTAGAAACAATAAAGTTGATCTAGTTGTAGTTGATTATCTACAACTAATGTCAGATTCTTCAAAAGCAAGTATGGAAAATCGTCAATTGGAAGTAAGTAAGATTTCAAGATCCTTAAAGCAACTTGCTAGAGAATTAGAATGTCCTGTTTTAGCATTAAGCCAATTAAGTAGAAATGTTGAAAAACGGGAAGATAAAACTCCTATTTTGTCAGATTTAAGAGAATCAGGAACAATTGAACAAGATGCCGATATGGTTATTTTTTTACATAGAAAAGATTATTATAATAAGCACAACATTAATGATGACAATGATAAAGCAGAAGCTGTTGACAAAAGTGAAATTGTCAATGGCAGTTCTTCTTTAACCAATGTCATTGTAGCAAAAAATAGACATGGGGCTATTGGAATTGTACAACTATTATTCCAACCAGAATGCAACAGGTTTATTTATGAAAAAAAGAGAAATTAA", "aa": "MYLNDKPTSTISDITTIAKRFARNNKVDLVVVDYLQLMSDSSKASMENRQLEVSKISRSLKQLARELECPVLALSQLSRNVEKREDKTPILSDLRESGTIEQDADMVIFLHRKDYYNKHNINDDNDKAEAVDKSEIVNGSSSLTNVIVAKNRHGAIGIVQLLFQPECNRFIYEKKRN", "aa_hexdigest": "e010f659ebcd71b528a0515ddb9150bb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P211", "query_cov": 0.9322033898305084, "subject_cov": 0.9116022099447514, "identity": 0.6970000000000001, "score": 216.0, "evalue": 3.36e-69, "valid": false, "product": "Replicative DNA helicase", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_A0A449A6S1", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_A0A449A6S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A6S1" ], "product": "Replicative DNA helicase" }, "genes": [], "id": "AHCKFKLKGB_842", "locus": "GCA013367975_00836" }, { "type": "cds", "sequence": "contig_1", "start": 487347, "stop": 488183, "strand": "-", "frame": 2, "gene": "dnaB", "product": "replicative DNA helicase", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_B3PN54", "UniRef:UniRef90_D1J8H9" ], "nt": "ATGAACATTCAAGAAGAAAGAGAATTAGCAATTATTAATAATGAAACATCGTTATTAGGTTTATTAATTAGGGAACCAAATTGTTATTTAACAATTGCTGAAATTTTAAAACCTGAAATGTTTCATTTTAAAAATAATCAAATTCTTTTTAAATCTATTTCAGAAGTTCATATTGCCTCTAAACAATTTGATATTGCCAACTTATTGAGTTATTTAAATGACCAAAAATTAATTGATCATATTGGTTCATTTGGCTATAGTGGTAGTGAATATATTAGTTGGCTTACTAAAAACGCAGGTTTTAGTGCTGAACTAGAGTCGTATGCCAAAAAAATAATTGATCAATACAAGAAGGATCGCTTACGAGATTTATTGAATAGTTCAATAAATATTATGGAAAACAAACCTTATGAAATTTCAGATTTATTATCCAATTTACAATTGAATTTAATTAATCTAGATATTTCAGAAATAAATACTGCTTATGAACAAGTAGGAAATAAAGCAGATGAACTATTTAATAGAATAATGAGCAAAGAAAAATCAGATATTGGCGTTGGCCTTTCTTTTGGATTTCCTCCATTGGATGAATTAATTTTAGGTGCTAATCCCGGAGATTTGATAATTTTGGCAGCTCGCCCAGCTATGGGAAAAACTGCTTTTGCTTTAAATATAGCAAATAATGTTGCCAAAAACAATAAAACGGTGCTGTTTTTTTCTCTTGAAATGTCAAATTCCCAAATTGTTCAAAGAATGATGTCAATTGATTCTTTGGTTCCAATTAGTTTAATGAGAAAAAATGAAATCTCAACAGAAAATGCTAAAAATCTAGCCTGA", "aa": "MNIQEERELAIINNETSLLGLLIREPNCYLTIAEILKPEMFHFKNNQILFKSISEVHIASKQFDIANLLSYLNDQKLIDHIGSFGYSGSEYISWLTKNAGFSAELESYAKKIIDQYKKDRLRDLLNSSINIMENKPYEISDLLSNLQLNLINLDISEINTAYEQVGNKADELFNRIMSKEKSDIGVGLSFGFPPLDELILGANPGDLIILAARPAMGKTAFALNIANNVAKNNKTVLFFSLEMSNSQIVQRMMSIDSLVPISLMRKNEISTENAKNLA", "aa_hexdigest": "7bab48241bb0fc5d133b0240a0bc4a19", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "dnaB" ], "truncated": "5-prime", "pseudogene": { "start": 487044, "stop": 488183, "observations": { "mutation": [ 487325, 487349 ], "stop": [ 487325, 487349 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H9", "query_cov": 1.0, "subject_cov": 0.5978494623655914, "identity": 0.996, "score": 534.0, "evalue": 1.52e-188, "gene_start": 1, "gene_end": 278, "reference_start": 1, "reference_end": 278, "reference_sequence": "MNIQEERELAIINNETSLLGLLIREPNCYLTIAEILKPEMFHFKNNQILFKSISEVHIASKQFDIANLLSYLNDQKLIDHIGSFGYNGSEYISWLTKNAGFSAELESYAKKIIDQYKKDRLRDLLNSSINIMENKPYEISDLLSNLQLNLINLDISEINTAYEQVGNKADELFNRIMSKEKSDIGVGLSFGFPPLDELILGANPGDLIILAARPAMGKTAFALNIANNVAKNNKTVLFFSLEMSNSQIVQRMMSIDSLVPISLMRKNEISTENAKNLAWTVSEMKQWKMYLNDKPTSTISDITTIAKRFARNNKVDLVVVDYLQLMSDSSKASMENRQLEVSKISRSLKQLARELECPVLALSQLSRNVEKREDKTPILSDLRESGTIEQDADMVIFLHRKDYYNKHNINDDNDKVEAIDKSEIVNGSSSLTNVIVAKNRHGAIGIVQLLFQPECNRFIYEKKKN" }, "paralog": false, "identity": 0.9920844327176781, "score": 719.0, "evalue": 7.13e-264, "description": "Internal stop codon at 487325, 487349. Nonsense mutation around 487325, 487349.", "psc": { "uniref90_id": "UniRef90_D1J8H9", "gene": "dnaB", "product": "replicative DNA helicase", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_B3PN54", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H9", "EC:5.6.2.3", "UniRef:UniRef50_B3PN54", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN54" ], "product": "Replicative DNA helicase" } }, "id": "AHCKFKLKGB_843", "locus": "GCA013367975_00837" }, { "type": "cds", "sequence": "contig_1", "start": 488209, "stop": 488649, "strand": "-", "frame": 1, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_036432579.1", "SO:0001217", "UniParc:UPI000512F987", "UniRef:UniRef100_UPI000512F987", "UniRef:UniRef50_A0A318U7T8", "UniRef:UniRef90_D1J8I0" ], "nt": "ATGAAAGTAATATTAATTAAAGAATACCAAAAACATAAAAAAAATGAAATCATTGAAGTTACAGACGGTTTTGCAAAAAACTTTTTAATTAAAAACGGTTATGCTCAACCAATTAACAAACAAACATTGGCAAATTTAGATAGAGTTAAATCAAATATTGCAGAAGACTTAGCAAATCAAATAGCTAAAGCTAATGAAATCAAACAATTAATTGAATCATTAGTTTTAAAATTCTATTTAAAATCTAATGGAAATATAGTGCACGGAAATGTTACAACAAAAGCAATTGAAAAAGAATTAATAAAAAACAACATTAAAATCCCAAGCCATGCATTTGAAGCAGGGGTAACATATAATACTTTTGGATTGCACGATGTAAAAATTAAATTACATGACCAAGTAACTGCAACACTAAAAATAAATATCATAGAAGAGAAATAA", "aa": "MKVILIKEYQKHKKNEIIEVTDGFAKNFLIKNGYAQPINKQTLANLDRVKSNIAEDLANQIAKANEIKQLIESLVLKFYLKSNGNIVHGNVTTKAIEKELIKNNIKIPSHAFEAGVTYNTFGLHDVKIKLHDQVTATLKINIIEEK", "aa_hexdigest": "b72e7da703915f031cf4125f45431109", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000512F987", "ncbi_nrp_id": "WP_036432579.1", "uniref100_id": "UniRef100_UPI000512F987", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512F987", "RefSeq:WP_036432579.1", "UniRef:UniRef100_UPI000512F987" ] }, "ips": { "uniref100_id": "UniRef100_UPI000512F987", "uniref90_id": "UniRef90_D1J8I0" }, "psc": { "uniref90_id": "UniRef90_D1J8I0", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_A0A318U7T8", "cog_id": "COG0359", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318U7T8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318U7T8" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "AHCKFKLKGB_844", "locus": "GCA013367975_00838" }, { "type": "cds", "sequence": "contig_1", "start": 488630, "stop": 488953, "strand": "-", "frame": 3, "gene": null, "product": "DHHA1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9M3" ], "nt": "TTGCAAGAAATTAAAAAAGGATATTTCTTGGCATACAAAGATATTGAAGCATCAACAGATATTATTTCAATAGCGTCAGATGAAATATTAAGAGTTAATGGTAGGAAAGCTGCATTTGTTGTTGCTAAATTACCAGGAACAAAAACATACAAAATGTCAGCAAGAGGAATTAATACAAATGTTCAAATTATAGCAGAAGCAGTTAACGGCGGTGGCCATTTTGGGGCAGCCGCTGCTGAAACAACTGAAAATTTGAATTTATTTGTTGATAATATAATACAAGCAATAGTGAGTGTGAAAGATAATGAAAGTAATATTAATTAA", "aa": "MQEIKKGYFLAYKDIEASTDIISIASDEILRVNGRKAAFVVAKLPGTKTYKMSARGINTNVQIIAEAVNGGGHFGAAAAETTENLNLFVDNIIQAIVSVKDNESNIN", "aa_hexdigest": "203427b7f42c4d179281510b7caf3d55", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P200", "query_cov": 1.0, "subject_cov": 1.009433962264151, "identity": 0.757, "score": 152.0, "evalue": 7.27e-46, "valid": false, "product": "Putative bifunctional signaling protein/50S ribosomal protein L9", "uniref50_id": "UniRef50_A0A432F9M3" }, "pscc": { "uniref50_id": "UniRef50_A0A432F9M3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9M3" ], "product": "DHHA1 domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_845", "locus": "GCA013367975_00839" }, { "type": "cds", "sequence": "contig_1", "start": 489035, "stop": 490198, "strand": "-", "frame": 3, "gene": "gdpP", "product": "GGDEF domain-containing protein", "db_xrefs": [ "COG:COG3887", "COG:T", "EC:3.1.4.59", "KEGG:K22927", "SO:0001217", "UniRef:UniRef50_A0A448ZXC6", "UniRef:UniRef90_D1J8I1" ], "nt": "ATGTATGAAATAGATGGATGCAATTACGAAGTTCATATATCATTTGAAAAAAATCTAGTTATTTTAAGAGATGTTACTGCCCAACAAAATTTATTAGATGACTATAAAAAACAAAGAATTGTGTTTGGGGAATTAGACATTGACAATATTAATTTATATCAAACATTTTTATCCCAAGAAGAAATTTTTAAAATTTATGCTTCTGTAGTAAATTTATTAGATGAAATATCTAAAAAATACAATGTTATTTATAGACAATATGAAAACGGAAGATTCTTTTTAATAACAAATAAAGAAACATTAGAAGAATTAGAACGAGATAGTTTTAATGTTATAACTTCAGTAAAAAACAAAATAATTGTTAAAGATGTTTTGATATCAATTTCTGGTGGATTTGGTTATGGTATTTTTAAATACGATACGTTAGTCAAATTAGCAAAAGATGCTCTAATGCAATCTCAAATGCGGGGTGGCGATCAGATTACAGTTTTAACAAAAAATGAAAAAGCTAGACACTATGGCTCAAGCTCGGAAATCGATGTAAATCTATCAAGGACAAATGTAAACTATATAGCGCAAAACTTATTAGAAAAGATAAAATCAAAAGATATTGAAAATGTTATAGCCTATGGCCATAAAAATGCAGACCTTGATGCCTTGGGTTCAACATATGCAATTTTGCAATTAGCATTAGCCTATAAAAAGAATGCTTATATTCAAAACAAGACATTTGATAGTACTACTCAAAGAGTTATTGATAAAATGTCCAAGGAAACTCAAGAATATTTTATTAGTCCTAAAGAAGCAGAAAGCATCAACAACGAAAAAACGTTGGTTGTAATTTGCGATACTTCAGATGATACTAGAATTGAAAATGCTAATGCCTTTAAAAACATAAAAAAAGAAAATATCATGATAATCGATCATCATAGAATAAATAAAAATACTGATGAAATAGCATATAGAGAAAATGTATATATTGATTCCTCGGCGTCATCTGCTTCTGAAATATTAACAGAAATCATCACATTGTTACACGAAGAAGATAAAATTAATAATGATACAGCGCAATATTTATTAGATGGAATTTATTTAGATACTAATACATTTAAAAAACAAACAAGTGCTAAAACTTTTGCAGCAGCATCAATATTGCAAAAATGA", "aa": "MYEIDGCNYEVHISFEKNLVILRDVTAQQNLLDDYKKQRIVFGELDIDNINLYQTFLSQEEIFKIYASVVNLLDEISKKYNVIYRQYENGRFFLITNKETLEELERDSFNVITSVKNKIIVKDVLISISGGFGYGIFKYDTLVKLAKDALMQSQMRGGDQITVLTKNEKARHYGSSSEIDVNLSRTNVNYIAQNLLEKIKSKDIENVIAYGHKNADLDALGSTYAILQLALAYKKNAYIQNKTFDSTTQRVIDKMSKETQEYFISPKEAESINNEKTLVVICDTSDDTRIENANAFKNIKKENIMIIDHHRINKNTDEIAYRENVYIDSSASSASEILTEIITLLHEEDKINNDTAQYLLDGIYLDTNTFKKQTSAKTFAAASILQK", "aa_hexdigest": "829c75af3e5ae77bb85faa833d2a6455", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [ "gdpP" ], "truncated": "both", "pseudogene": { "start": 488732, "stop": 490498, "observations": { "mutation": [ 489037, 490222, 490303 ], "stop": [ 489037, 490222, 490303 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I1", "query_cov": 1.0, "subject_cov": 0.589041095890411, "identity": 0.997, "score": 743.0, "evalue": 2.89e-266, "gene_start": 1, "gene_end": 387, "reference_start": 136, "reference_end": 522, "reference_sequence": "MKNKDIQKIYIALQVILLFILPTILFFIGIFCLKTQQSKIALMAIYTLFLIGLAIYLTIILQRYFKNSILAKKSLEFYLQSEFNQFGTGILIFLNSGKIIWASESLKIKFHNNLINKNIKDIFNIKQWTSDNQEFMYEIDGCNYEVHISFEKNLVILRDVTAQQNLLDDYKKQRIVFGELDIDNINLYQTFLSQEEIFKIYASVVNLLDEISKKYNVIYRQYENGRFFLITNKETLEELERDSFNVITSIKNKIIVKDVLISISGGFGYGIFKYDTLVKLAKDALMQSQMRGGDQITVLTKNEKARHYGSSSEIDVNLSRTNVNYIAQNLLEKIKSKDIENVIAYGHKNADLDALGSTYAILQLALAYKKNAYIQNKTFDSTTQRVIDKMSKETQEYFISPKEAESINNEKTLVVICDTSDDTRIENANAFKNIKKENIMIIDHHRINKNTDEIAYRENVYIDSSASSASEILTEIITLLHEEDKINNDTAQYLLDGIYLDTNTFKKQTSAKTFAAASILQKWGAQNDKSVDTLKFDEKVFKIVQELLANLQEIKKGYFLAYKDIEASTDIISIASDEILRVNGRKAAFVVAKLPGTKTYKMSARGINTNVQIIAEAVNGGGHFGAAAAETTENLNLFVDNIIQAIVSVKDNESNIN" }, "paralog": false, "identity": 0.9914965986394558, "score": 1123.0, "evalue": 0.0, "description": "Internal stop codon at 489037, 490222, 490303. Nonsense mutation around 489037, 490222, 490303.", "psc": { "uniref90_id": "UniRef90_D1J8I1", "gene": "GdpP", "product": "GGDEF domain-containing protein", "ec_ids": [ "3.1.4.59" ], "uniref50_id": "UniRef50_A0A448ZXC6", "cog_id": "COG3887", "cog_category": "T", "kegg_orthology_id": "K22927", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I1", "EC:3.1.4.59", "UniRef:UniRef50_A0A448ZXC6", "COG:COG3887", "COG:T", "KEGG:K22927" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXC6" ], "product": "Probable manganese-dependent inorganic pyrophosphatase" } }, "id": "AHCKFKLKGB_846", "locus": "GCA013367975_00840" }, { "type": "cds", "sequence": "contig_1", "start": 490301, "stop": 490603, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACAAAGATATTCAAAAAATTTATATAGCTTTACAAGTTATCCTATTATTTATTTTGCCAACAATATTATTCTTTATTGGCATTTTTTGTTTAAAGACTCAACAGTCAAAAATAGCGTTAATGGCAATTTATACTTTATTTTTAATCGGCCTTGCAATTTATTTAACTATTATTTTGCAACGTCATTTTAAAAACTCAATATTAGCTAAAAAATCATTGGAATTTTATTTACAAAGCGAATTTAATCAATTTGGTACGGGAATTTTGATATTTTTAAATAGTGGAAAAATTATATGA", "aa": "MKNKDIQKIYIALQVILLFILPTILFFIGIFCLKTQQSKIALMAIYTLFLIGLAIYLTIILQRHFKNSILAKKSLEFYLQSEFNQFGTGILIFLNSGKII", "aa_hexdigest": "8fcf6fa3d8cb8f4effa2b48a8b43adfc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11532.958699999985, "isoelectric_point": 9.813909339904786 }, "id": "AHCKFKLKGB_847", "locus": "GCA013367975_00841" }, { "type": "cds", "sequence": "contig_1", "start": 490657, "stop": 490941, "strand": "-", "frame": 1, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "SO:0001217", "UniRef:UniRef50_B3PN57", "UniRef:UniRef90_D1J8I2" ], "nt": "ATGATTGACTTTTATTTTAAAAACGATAAAAACAATAAAAAAGTGTTGCTATTAATTGATGCTAAAATTGGTTTTAGCGCTAGTGATTTAGAAATAATAGAATATCTTAACGATCTTAAAATAAAATTTGATATAGTTGTTACAAAAATCGACAAGGCAAATCAATCTCAACGTTATTGCGTTAAAAATCAAGCCTTGTCTTTAAAAACAGCTGAAAATATTTTTTTAGTATCATCTGAAAAAAAACAAGGTATTGAAGAAATAATGCAATTTTATGACTTGTAG", "aa": "MIDFYFKNDKNNKKVLLLIDAKIGFSASDLEIIEYLNDLKIKFDIVVTKIDKANQSQRYCVKNQALSLKTAENIFLVSSEKKQGIEEIMQFYDL", "aa_hexdigest": "7bbafeda14442b568ac563ccdcc705ea", "start_type": "ATG", "rbs_motif": null, "genes": [ "yihA" ], "truncated": "3-prime", "pseudogene": { "start": 490657, "stop": 491208, "observations": { "mutation": [ 491085, 491136, 491205 ], "stop": [ 491085, 491136, 491205 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I2", "query_cov": 1.0, "subject_cov": 0.5136612021857924, "identity": 0.9890000000000001, "score": 182.0, "evalue": 3.15e-57, "gene_start": 1, "gene_end": 94, "reference_start": 90, "reference_end": 183, "reference_sequence": "MWKFLKSCKSHDDFINSDNIQYVFWGRSNVGKSSLINSLASCNIAKTSSYPGRTQLINYFESNKNKIIIDLPGYGYAKISKKLQDKISFMIDFYFKNDKNNKKVLLLIDAKIGFSASDLEIIEYLNDLKIKFDIVVTKIDKANQSQRYCVKNQALSLKTVENIFLVSSEKKQGIEEIMQFYDL" }, "paralog": false, "identity": 0.9781420765027322, "score": 348.0, "evalue": 7.5e-125, "description": "Internal stop codon at 491085, 491136, 491205. Nonsense mutation around 491085, 491136, 491205.", "psc": { "uniref90_id": "UniRef90_D1J8I2", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_B3PN57", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I2", "UniRef:UniRef50_B3PN57", "COG:COG0218", "COG:D", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN57" ], "product": "Probable GTP-binding protein EngB" } }, "id": "AHCKFKLKGB_848", "locus": "GCA013367975_00842" }, { "type": "cds", "sequence": "contig_1", "start": 491208, "stop": 491594, "strand": "-", "frame": 2, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q6KIG0", "UniRef:UniRef90_D1J8I3" ], "nt": "ATGGAATTTAGAGATAAATACACAGAACATTTTACTATTCCACATACTGAAGCTCAATTGAACTTTTCATTTAGTGGATTAAAAACACAAGTTATAAATCTTATAAATAACCAAATCAATAGAAAACAAGAGATCAATGTTGATCAATATGCAACAATGTTTGAAAATACAGTAGTCTTATATTTAAAAAATCATTTAGAAATTGCAATTAAAAAATATCATCCAAGAGTAATTGTTTTGGCAGGCGGAGTAAGTGCTAATAAAGCAATTAGAGAAATGTTTTTAAATGTCCATTCAAATGCCATAATTCCGGAAATGAAATATACAACTGATAATGCAATGATGATTGCTAGGTTAGCATATGAACTATTAAGGAGAGATAGATAA", "aa": "MEFRDKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR", "aa_hexdigest": "e3577e7b0c508003aa3e90d94bf16fc9", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 491208, "stop": 491894, "observations": { "mutation": [ 491597 ], "stop": [ 491597 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I3", "query_cov": 1.0, "subject_cov": 0.4115755627009646, "identity": 1.0, "score": 252.0, "evalue": 3.28e-82, "gene_start": 1, "gene_end": 128, "reference_start": 184, "reference_end": 311, "reference_sequence": "MLIFAIESSHDDTSFALLEDNKPIWMETISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKKWMEFRDKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR" }, "paralog": false, "identity": 0.9956140350877193, "score": 450.0, "evalue": 8.81e-163, "description": "Internal stop codon at 491597. Nonsense mutation around 491597.", "psc": { "uniref90_id": "UniRef90_D1J8I3", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q6KIG0", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I3", "EC:2.3.1.234", "UniRef:UniRef50_Q6KIG0", "COG:COG0533", "COG:J", "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG0" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "AHCKFKLKGB_849", "locus": "GCA013367975_00843" }, { "type": "cds", "sequence": "contig_1", "start": 491595, "stop": 492068, "strand": "-", "frame": 2, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q6KIG0", "UniRef:UniRef90_D1J8I3" ], "nt": "ATGGAAACAATTAGCCAAATAGACATTCATAGAAAATATGGAGGCACAGTTCCAGAATTAGCATCAAGATTGCATGTAAAAAATATAGGTATTTTAATTGAAAAATTAAAAAAAATAATTGATATTAAAAAGATTGACTATATAGCATATACAAAAGAACCAGGATTAGTTGGTTCTTTACATGTCGGATATGTTGTTGCTAATGCAATAGCAATGTTACTTGAAAAACCTATATTGCCTTTAAATCATTTAGAAGGGCATTTTTTCTCTGCTTTTATAGGCAAAGAAGTTGAATACCCTGCTTTAGGTTTAATTGTTTCAGGTGGGCATTCACAATTAATGTTATATAAATCAAAAAATGAATACGAAGTTATAGGTCAAACTCAAGATGATGCCGTCGGAGAAGTATTTGATAAAATAGCAAGAAAATTAAATTTGGGTTTTCCTGGAGGACCTATTATTGATAAAAAATGA", "aa": "METISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYIAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKK", "aa_hexdigest": "15c8610418188d4a5af53ff12e638e58", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "both", "pseudogene": { "start": 491292, "stop": 492143, "observations": { "mutation": [ 491597, 492071 ], "stop": [ 491597, 492071 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I3", "query_cov": 1.0, "subject_cov": 0.5048231511254019, "identity": 0.9940000000000001, "score": 314.0, "evalue": 3.8e-106, "gene_start": 1, "gene_end": 157, "reference_start": 26, "reference_end": 182, "reference_sequence": "MLIFAIESSHDDTSFALLEDNKPIWMETISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKKWMEFRDKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR" }, "paralog": false, "identity": 0.9893992932862191, "score": 553.0, "evalue": 4.35e-203, "description": "Internal stop codon at 491597, 492071. Nonsense mutation around 491597, 492071.", "psc": { "uniref90_id": "UniRef90_D1J8I3", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q6KIG0", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I3", "EC:2.3.1.234", "UniRef:UniRef50_Q6KIG0", "COG:COG0533", "COG:J", "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG0" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "AHCKFKLKGB_850", "locus": "GCA013367975_00844" }, { "type": "cds", "sequence": "contig_1", "start": 492137, "stop": 492691, "strand": "-", "frame": 3, "gene": "tsaB", "product": "Gcp-like domain-containing protein", "db_xrefs": [ "COG:COG1214", "COG:J", "RefSeq:WP_012855670.1", "SO:0001217", "UniParc:UPI0001BC23D2", "UniRef:UniRef100_D1J8I4", "UniRef:UniRef50_D1J8I4", "UniRef:UniRef90_D1J8I4" ], "nt": "GTGAAATTATTCATTGAAACTTCATTAAGCGATTTTTATATAAGCTTAATTGATGAAAATTATTGCGAGATTGATTGTATTTTTATTGCTAACTTAGTTAAAAAAACCGATGCATTTTTTGAAAATTTAAATTATTTATTAAATAAAAATAATTTTAAATTAAGTTCAATTTCAGAAATTTATACAACGTTAGGACCTGGGAGTTTTTCAGGTGCAAGAATTGGCTTAGTTTTTGCCAAAACAGTAGCACAATTATCAAATATCAAATTATTTGTAGCCCCAACATATTCTTTATTTTATACACAAAAAAAATTGCAACATGAAGATTGCAATTTAATAAATATAAAAGCCAATAAATACAATGTTTATTCAATAATTGTTGATCCATTTGAATGTCATATGCAAGAAAACTTAGGCAATTTTGATTTATTCAATAAAGAGCTTTTTTCAAAAAATATAGAGCAATATTTAAAAGAATTTAAACAAGTAAATGATATTGAAAATTGCGAATTAATTTATTTGCATGATCCACAAATAGGAGAGATAAAATGTTAA", "aa": "MKLFIETSLSDFYISLIDENYCEIDCIFIANLVKKTDAFFENLNYLLNKNNFKLSSISEIYTTLGPGSFSGARIGLVFAKTVAQLSNIKLFVAPTYSLFYTQKKLQHEDCNLINIKANKYNVYSIIVDPFECHMQENLGNFDLFNKELFSKNIEQYLKEFKQVNDIENCELIYLHDPQIGEIKC", "aa_hexdigest": "f5bc757e9aebf1457d73e00cc13b0baa", "start_type": "GTG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001BC23D2", "ncbi_nrp_id": "WP_012855670.1", "uniref100_id": "UniRef100_D1J8I4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC23D2", "RefSeq:WP_012855670.1", "UniRef:UniRef100_D1J8I4" ] }, "ips": { "uniref100_id": "UniRef100_D1J8I4", "uniref90_id": "UniRef90_D1J8I4" }, "psc": { "uniref90_id": "UniRef90_D1J8I4", "gene": "TsaB", "product": "Gcp-like domain-containing protein", "uniref50_id": "UniRef50_D1J8I4", "cog_id": "COG1214", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J8I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8I4" ], "product": "Gcp-like domain-containing protein" }, "genes": [ "tsaB" ], "id": "AHCKFKLKGB_851", "locus": "GCA013367975_00845" }, { "type": "cds", "sequence": "contig_1", "start": 492793, "stop": 493089, "strand": "-", "frame": 1, "gene": "tsaE", "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE", "db_xrefs": [ "COG:COG0802", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A3G8LG14", "UniRef:UniRef90_D1J8I5" ], "nt": "ATGTCAAATCTAAAATTTATTTATAAAAATAACGAACCATTAGATAAACTAGTTGACTATATTTTGTCATTTAAAAATTTAGAAGCCTTGCTTTTAATAGGCGAACTTGGAGCAGGCAAAACAACTTTAACAAGTCAAATAGCAAAAAAATTAAACGAACCAAAGACTGTAATATCACCCACATTCAATACAATTTTAGTGTATGATAAATTTGTACATATTGATGCTTATAAATTAACTACAAATTTATTTGCTTATGAAGATTATTTTGAAGATAAATTAGCAATTATTGAATGA", "aa": "MSNLKFIYKNNEPLDKLVDYILSFKNLEALLLIGELGAGKTTLTSQIAKKLNEPKTVISPTFNTILVYDKFVHIDAYKLTTNLFAYEDYFEDKLAIIE", "aa_hexdigest": "d4dfdacce5f9d287a456a83f5ae9929e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "tsaE" ], "truncated": "5-prime", "pseudogene": { "start": 492691, "stop": 493095, "observations": { "mutation": [ 492795 ], "stop": [ 492795 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I5", "query_cov": 1.0, "subject_cov": 0.7313432835820896, "identity": 1.0, "score": 191.0, "evalue": 3.45e-61, "gene_start": 1, "gene_end": 98, "reference_start": 3, "reference_end": 100, "reference_sequence": "EIMSNLKFIYKNNEPLDKLVDYILSFKNLEALLLIGELGAGKTTLTSQIAKKLNEPKTVISPTFNTILVYDKFVHIDAYKLTTNLFAYEDYFEDKLAIIEWAENVTLPKFKHYIKIKVYFDNEQNHVFEVMEEQ" }, "paralog": false, "identity": 0.9850746268656716, "score": 264.0, "evalue": 1.57e-92, "description": "Internal stop codon at 492795. Nonsense mutation around 492795.", "psc": { "uniref90_id": "UniRef90_D1J8I5", "gene": "TsaE", "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE", "uniref50_id": "UniRef50_A0A3G8LG14", "cog_id": "COG0802", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I5", "UniRef:UniRef50_A0A3G8LG14", "COG:COG0802", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LG14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LG14" ], "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE" } }, "id": "AHCKFKLKGB_852", "locus": "GCA013367975_00846" }, { "type": "cds", "sequence": "contig_1", "start": 493273, "stop": 493962, "strand": "-", "frame": 1, "gene": "ysdC", "product": "Aminopeptidase ysdC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "nt": "ATGCAAGAAGAAGAAAGAAAGAAAGCAATTACAGAAAATGAATTATTTGCTGATTTTTGTTTCAAAAGCGATGAAGATGCTATTGCTCATGGTGTTAATGTTGGCGATAGAGTTTACATGAGCGGAGAAACAATACTATTTAATGATCAAAATTTAATTGGTGGAAAAGCAATGGACAACCGTGCTGGTGTAACCGTGTTAGAATTTGTTGCTAAAGCTGTTGCAAAATTAGATCTTGACATTGATCTATATTTAGTAGGTACAGTTCAAGAAGAAGTTGGAACAAGAGGAGCTAAAACAGTTGCATCTATGATTGATCCAGACATTGCAATAGCAGTTGATACTTCTGCATCTCACGATACACCTAATACAATTCCTGGAAATGTTTCTTTATATAAGGGAGCTGCTTTAAGATTAAAAGATAGAGGAACAATGATGGATCCAAAATTGGTACAATTTTTTGTTGATTTATCTAAAAAATATAATATTCCAACATTTAAATATGTAGCCATGGGTGGTGGTACTGATGCCTTTGAATTACAATTTTCAAAAGGTGGGGCAGCCACATTAACAATTTCATTACCTCAAAGATATTTGCATTCACCAATTGGAGTATGTTGCATAAGTGATTTATTGGCAGCCGTTGATTTGTTAACAAAATTTTTAACTGATTTTAAACAATCTGATTGA", "aa": "MQEEERKKAITENELFADFCFKSDEDAIAHGVNVGDRVYMSGETILFNDQNLIGGKAMDNRAGVTVLEFVAKAVAKLDLDIDLYLVGTVQEEVGTRGAKTVASMIDPDIAIAVDTSASHDTPNTIPGNVSLYKGAALRLKDRGTMMDPKLVQFFVDLSKKYNIPTFKYVAMGGGTDAFELQFSKGGAATLTISLPQRYLHSPIGVCCISDLLAAVDLLTKFLTDFKQSD", "aa_hexdigest": "0862365152d7619311343c643af321a6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE1AB9A", "query_cov": 0.8733624454148472, "subject_cov": 0.9478672985781991, "identity": 0.735, "score": 297.0, "evalue": 7.82e-100, "valid": false, "product": "M42 glutamyl aminopeptidase", "uniref50_id": "UniRef50_A0A3B0P1S5" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P1S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "product": "Aminopeptidase ysdC" }, "genes": [ "ysdC" ], "id": "AHCKFKLKGB_853", "locus": "GCA013367975_00847" }, { "type": "cds", "sequence": "contig_1", "start": 494053, "stop": 494337, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGAGAAAATCAATTCAAAGAAAGATTAATAAAATATATGGAAATTGAAGCAATGTCTCGTTATGAAGAACCAGTTGTTAATGAATTAAAAAAAGAATTAGCTAATGAAGGATTTGAATTTAGCAGAGATAATTTTGGCTGTTTAATTATGCACAAGCCATCTAAAAATCCAAATGCTCCTAAAGTTTTATTAGCAGCACACATGGATGAAGTTGGATATGTAGTAAGAAATTTTGATAAAAAAGGACAATTATATTTATCAACAGTTGGTGGTGTATGA", "aa": "MTRENQFKERLIKYMEIEAMSRYEEPVVNELKKELANEGFEFSRDNFGCLIMHKPSKNPNAPKVLLAAHMDEVGYVVRNFDKKGQLYLSTVGGV", "aa_hexdigest": "3fc428d1e9f98d963fffb68baebbdbb2", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10869.407299999992, "isoelectric_point": 6.733567619323731 }, "id": "AHCKFKLKGB_854", "locus": "GCA013367975_00848" }, { "type": "rRNA", "sequence": "contig_1", "start": 494473, "stop": 497368, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9900854700854701, "score": 2764.0, "evalue": 4.4e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TATTAAACAAGCAAGAGTTTTTGGTGGATGCCTTGGGTCTGGAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGGTTAGCTGGAAATAAGCTGTTATCCGGGGATTTCCGAATGGGGAAACCTAATTGAGCTAATCCTCAATTATCATATCGATGAATTCATAGTCGAATGAAGAGACACGCTGTGAATTGAAACATCTCAGTAGCAGCAGGAAGAGAAAATAAAGAATGATTCCCTAAGTAGTGGCGAGCGAACGGGGAAGAGCCCAAACCAACACATGTTGTTGGGGTTGTAGGACTGCGACATGGATTAATGAATTTGTACATAGCAGAATAAGTTGGAACGCTTAAACATAGAGGGTGAAATTCCCGTAAGCGAAATGTATAAATCTCCTAGCAGTATCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATTTGCCGGGACCACCCGGTAAGGCTAAATACTAACCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGGGAGTGAAATAGATTCTGAAACCAATTACTTACAGTTAGTCAGAGCCCGTTAATGGGTGATGGCGTACATCTTACAGAATGGACCGGCGAGTTATGTCAACATGCGAGGTTAAGTAGAATAAAGCGAAGCCGTAGAGAAATCGAGTCTGAATAGGGCGATTTAGTATGTTGATATAGACCCGAAACCAGGTGATCTACCCATGAGCAGGTTGAAACTTAGGTAACACTAAGTGGAGGACCGAACCGCAGTACGCTAAAAAGTGCCCGGATGACTTGTGGGTAGGGGTGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAATGATGGGGGTAGAGCACTGAATATGGAATGGCGGCGCCTAGCCGTACTGACTATAATCAAACTCCGAATACTATCATGTATTACTATGCAGTCGGTACATCGGTGATAACGTCGATGCACGCGAGGGGAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGTGTTAAGTGAGAAAGGTTGTGAAGTTTCTTAAACAGCTAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAATAGCTCACTAGTCGAGAGACTTTGTGCCGATAATTCAACGGGACTAAAACACAATACCGAAGCTACGGGCAGAAATGCGTTAGGAGAGCGTTGTAAGGGCTTAGAAGCCAGACCGTGAGGACTGGTGGAGCGCTTACAAGTGAGAATGCCGGTATGAGTAACGATTCAGAGTGAGAATCTCTGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCGGGTCCTAAGACGAGGCCGAAAGGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTTAACATGTGATGGAGTGACGGGGAAGGATAATTTTACCACTAATTGGATTGTGGGGTAAGTAACAACTGGGTTATGTAGGCAAATCCGCATAACTTAACTGGGAGTTACGACGCATAGCAAAAGGGCAACTGAGTAGCGAATTAGATGATTTCATGCCTCTTAAAAAAGCTTCTAACATTAAGTGTTAGGAAACCCGTACCGAGAACGGACACACGTCCCCAAGATGAGTATTCTAAGGCGAGCGAGAAAACTATTGTCAAGGAACTCTGCAAATTCATCCCGTAAGTTCGCAAGAAGGGATGCCCACAATAAAATGTGGGCCGCAGTGAATAGTAAGGGGGAACTGTTTATCAAAAACACAGCTCTATGCTAAGTCGTAAGATGATGTATATGGGGTGACTCCTGCCCAGTGCCCGAAGGTTAAGCAGAGGTGTTAGCATTAGCGAAGCATTAATGTGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGAGTAATTATCTCTTAACTGTCTCGACAATAGACTCGGTGAAATTATGGTTCCGGCGAAGACGCCGGAGACCCGCATCTAGACGAAAAGACCCCGTGGAGCTTTACTATAACTTCATATTGGAGTTTGATTTAACATGTGTAGGATAGGTGGGAGACGTTGATGCTTGAACGCTAGTTTAAGAGTAGTCGCCGTTGAAATACCACCCTTGTTACATTGAACTTCTAACTTGTTCCCGTCATCCGGGAAGAGGACAGTGTGTGGTGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGGGTAACGGAGGCGTTCAAAGTTACACTCAATACGGTCAGAAACCGTATCTAAGAGCATAAAGGTAGAAGTGTGATTGACTGTGAGACCTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCGGTTCTTCGTGGAAAGGCCGTCGCTCAACGGATAAAAGCTACCCCGGGGATAACAGGCTTATCTTTCCCAAGAGATCACATCGACGGGAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTTCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCTACTCTTAGTACGAGAGGACCGGAGTGGACGCACCGATGGTGTGCCAGTTGTTTCGCCAGAAGCATAGCTGGGTAGCCAAGTGCGGCAGGGATAACCGCTGAAAGCATCTAAGCGGGAAGCCCCCTCAAAGATTAGTATTCCCTTTAAAATTCCTTATAGACTATGAGGTTGATAGGCTGGAGGTGTAAGTGCAGCAATGCATTCAGCTGACCAGTACTAATAAATTTATTGGTTTAATAGT", "id": "AHCKFKLKGB_39", "locus": "GCA013367975_00849" }, { "type": "rRNA", "sequence": "contig_1", "start": 497552, "stop": 499069, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1513.0, "evalue": 2.3e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTATAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAGGTTAGCAATAACCTAGCGGCGAATGGGTGAGTAACACGTGCTTAATCTACCTTTTAGATTGGAATACCCATTGGAAACAATGGCTAATGCCGGATACGCATGGAACCGCATGGTTCCGTTGTGAAAGGCGCTGCAAGGCGCCACTAAAAGATGAGGGTGCGGAACATTAGTTAGTTGGTGAGGTAATGGCCCACCAAGACTATGATGTTTAGCCGGGTCGAGAGACTGAACGGCCACATTGGGACTGAGATACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCACGATGAAGGTCTTCGGATTGTAAAGTGCTGTTATAAGGGAAGAACATTTGCAATAGGAAATGATTGCAGACTGACGGTACCTTGTCAGAAAGCGATGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTTCGTAGGCTGTTTGTTAAGTCTGGAGTTAAATCCCGGGGCTCAACCCCGGCTCGCTTTGGATACTAGCAAACTAGAGTTAGATAGAGGTAAGCGGAATTCCATGTGAAGCGGTGAAATGCGTAGATATATGGAAGAACACCAAAGGCGAAGGCAGCTTACTGGGTCTATACTGACGCTGAGGGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTCGGTGGAGAATCACTGACGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACACGGAAAACCTTACCCACTCTTGACATCCTTCGCAAAGCTATAGAGATATAGTGGAGGTTATCGGAGTGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTGCAACGAGCGCAACCCCTATCTTTAGTTACTAACATTAAGTTGAGGACTCTAGAGATACTGCCTGGGTAACTGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCCACACACGTGCTACAATGGTCGGTACAAAGAGAAGCAATATGGCGACATGGAGCAAATCTCAAAAAGCCGATCTCAGTTCGGATTGGAGTCTGCAATTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCTATGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATACCCAAAGTCGGTTTGCTAACCTCGGAGGCGACCGCCTAAGGTAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "AHCKFKLKGB_36", "locus": "GCA013367975_00850" }, { "type": "cds", "sequence": "contig_1", "start": 499345, "stop": 499635, "strand": "-", "frame": 1, "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "db_xrefs": [ "COG:COG0250", "COG:K", "GO:0006355", "KEGG:K02601", "SO:0001217", "UniRef:UniRef50_Q6KIE5", "UniRef:UniRef90_D1J8I7" ], "nt": "TTGATTGGTTCAAGTGGTAACAAAACAAAGCCTACTCCAATAAGTGATAAAAAATTTCAATTAATGATTGATAAAGAAGCAATGTTGCTAGCTAAATTTAAAGCAGGGGACATTGAAACTGCTTTTAAAGAAGGGGCTTTAGTAAAAATTTCTAGTGGAATATTCAAAGATGAAGTTGGCGAAATAATTAAAAATAATGATATTACCCAAAAAGCCTTTGTAAATATTGAACAATTTGGTAGAAAAGTTCCAACTGAATTTGATTATGCAGATCTAGAAATAGTAGGTTAA", "aa": "MIGSSGNKTKPTPISDKKFQLMIDKEAMLLAKFKAGDIETAFKEGALVKISSGIFKDEVGEIIKNNDITQKAFVNIEQFGRKVPTEFDYADLEIVG", "aa_hexdigest": "0e8c87d210692d92ff2c66bbff1138a7", "start_type": "TTG", "rbs_motif": null, "genes": [ "nusG" ], "truncated": "3-prime", "pseudogene": { "start": 499345, "stop": 499932, "observations": { "mutation": [ 499671, 499905 ], "stop": [ 499671, 499905 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I7", "query_cov": 1.0, "subject_cov": 0.49230769230769234, "identity": 0.9790000000000001, "score": 184.0, "evalue": 1.75e-57, "gene_start": 1, "gene_end": 96, "reference_start": 100, "reference_end": 195, "reference_sequence": "MELTNNDFKWYMISTVSGKEENVIESLKNKIAAQRMDEYFKDIRMFKEPHLSNKELEKKQRNEEFTVKFVNMYKGYIFLNMAMTDDTWYLVRNTQYVTGLIGSSGNKTKPTQISDKKFQLMIDKEAMLLAKFKAGDIETAFKEGALVKISSGIFKDEVGEIIKNNDITQKAFVNIEQFGRKVPTEFDYADLEIVG" }, "paralog": false, "identity": 0.9846153846153847, "score": 376.0, "evalue": 9.58e-136, "description": "Internal stop codon at 499671, 499905. Nonsense mutation around 499671, 499905.", "psc": { "uniref90_id": "UniRef90_D1J8I7", "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "uniref50_id": "UniRef50_Q6KIE5", "cog_id": "COG0250", "cog_category": "K", "kegg_orthology_id": "K02601", "go_ids": [ "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I7", "UniRef:UniRef50_Q6KIE5", "COG:COG0250", "COG:K", "KEGG:K02601", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIE5" ], "product": "Transcription termination/antitermination protein NusG" } }, "id": "AHCKFKLKGB_855", "locus": "GCA013367975_00851" }, { "type": "cds", "sequence": "contig_1", "start": 499669, "stop": 499899, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02357.24" ], "nt": "ATGATTTCAACAGTTTCAGGCAAAGAAGAAAATGTTATAGAATCATTAAAAAATAAAATTGCTGCTCAAAGAATGGATGAATACTTTAAAGATATAAGGATGTTTAAAGAACCCCATCTATCAAATAAAGAATTAGAAAAGAAGCAAAGAAACGAAGAATTTACTGTTAAATTTGTAAATATGTACAAAGGTTATATTTTTTTAAATATGGCAATGACTGATGATACATGA", "aa": "MISTVSGKEENVIESLKNKIAAQRMDEYFKDIRMFKEPHLSNKELEKKQRNEEFTVKFVNMYKGYIFLNMAMTDDT", "aa_hexdigest": "83445adab5e4fdbba14afdcaba235a99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02357.24", "name": "NusG", "length": 74, "aa_cov": 0.9736842105263158, "hmm_cov": 0.7432432432432432, "evalue": 2.1064364897283795e-08, "score": 29.945831298828125, "start": 2, "stop": 75 } ], "seq_stats": { "molecular_weight": 9073.367599999994, "isoelectric_point": 6.579306602478028 }, "id": "AHCKFKLKGB_856", "locus": "GCA013367975_00852" }, { "type": "cds", "sequence": "contig_1", "start": 500627, "stop": 501064, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5D951C", "UniRef:UniRef90_UPI001E5D951C" ], "nt": "TTGAACAATGATGAATGTAAGGAACTAGAAAAACAAATTGGAATTAATTTACATGAGGTTTGCAATTTATACAAGGCATGTAAAATATTAAAAACCAAAGTAGATGATATTGTTTATTTGCACATGAATAAGCATACTTTTTGGCATTTAAAATGCACAATTCTTATTAAAAAGAAATATAAAGAAATTTATTTTAAAAGACTAGAAATTTTTAATTTACTTGAAACCATCTTTAAAAAAGTGGCAGAAATTGATAAAAAAAGTGGAAATATACAAAAAATCGTAAATAAATACAAAATTAGTTTAAATGATTTATCAAATATAATGGCAGGTGAACATGAACATTTTCCTCAACACTTTATTGATAATTTAAAAGACAAAAAAACAATTATACAAAAAAAATTAGTTGATTTTAATAACGATGAATATCTAAAATGA", "aa": "MNNDECKELEKQIGINLHEVCNLYKACKILKTKVDDIVYLHMNKHTFWHLKCTILIKKKYKEIYFKRLEIFNLLETIFKKVAEIDKKSGNIQKIVNKYKISLNDLSNIMAGEHEHFPQHFIDNLKDKKTIIQKKLVDFNNDEYLK", "aa_hexdigest": "ae7434497fc3097ecd8cc647b152f69d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 500549, "stop": 501164, "observations": { "insertion": [ 501143 ], "mutation": [ 500573, 501062 ], "stop": [ 500573, 501062 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E5D951C", "query_cov": 1.0, "subject_cov": 0.7107843137254902, "identity": 0.966, "score": 288.0, "evalue": 9.78e-98, "gene_start": 1, "gene_end": 145, "reference_start": 27, "reference_end": 171, "reference_sequence": "MDNFINKIWTFINKAKNSYDKICQLSLNNDECKELEKQIGINLHEVCSLYKACKILKTKVDDIVFLHMNKHAFWHLKCTILIKKKYKEIYFKRLEIFNLLETIFKKVAEIDKKSGNIQKIVNKYKVSLNDLSNIMAGEHEHFPQHFIDNLKDKKTIIQKKLVDFNNDEYLKWNEKILKQNLLEIKNLIIEYAAFYFKFKTITRI" }, "paralog": false, "identity": 0.9607843137254902, "score": 394.0, "evalue": 4.17e-142, "description": "Internal stop codon at 500573, 501062. Frameshift due to insertion around 501143. Nonsense mutation around 500573, 501062.", "psc": { "uniref90_id": "UniRef90_UPI001E5D951C", "uniref50_id": "UniRef50_UPI001E5D951C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E5D951C", "UniRef:UniRef50_UPI001E5D951C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E5D951C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5D951C" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17384.2558, "isoelectric_point": 9.121003150939941 }, "id": "AHCKFKLKGB_857", "locus": "GCA013367975_00853" }, { "type": "cds", "sequence": "contig_1", "start": 501248, "stop": 501601, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGAAAAAGAATTTAAATTTAATTATGAGATAGAAGTTGAAAAAATAAAAACTTTGATTAACAAATGTCAAAATTCAAAAGATATTTCAAATATTGAACAAATCATTAATAAACTATTAGCTTTGATAAATACAACTAGTATAAAAATATATAACTATATTAAAACCTTTGCTTTTCATTTAAAAATACAAAATAATATAAAAAAAATCATTGAAAAAATTGATGTTCAAAATAAAAAAATTTCTGATTTCTTTGCAAGTGAAACTAAAAAGAATTTATTAAAAAGTGAATACCTTAATACTTATAAGCCAATATTGAATAGCTTTATAGATATTAAAAATAATAATTGA", "aa": "MLEKEFKFNYEIEVEKIKTLINKCQNSKDISNIEQIINKLLALINTTSIKIYNYIKTFAFHLKIQNNIKKIIEKIDVQNKKISDFFASETKKNLLKSEYLNTYKPILNSFIDIKNNN", "aa_hexdigest": "68d4d0a741e1c5706fcabcfe2a5d7051", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13879.059499999974, "isoelectric_point": 9.42735691070557 }, "id": "AHCKFKLKGB_858", "locus": "GCA013367975_00854" }, { "type": "cds", "sequence": "contig_1", "start": 501602, "stop": 502090, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D95B73", "UniRef:UniRef90_UPI0034D95B73" ], "nt": "ATGGATATAGTTGAATTTATAGAGTTGAAGAGATGGTTAGAATCGTTATTGAATTTGTTGAAGGTTCAAAATAAAATAATAAATAAAATAGACGAACAAGAATATCAAAACAATCAAATTGAATTTTATATTGAAAACTTTAAAAGATTAACTGCCAATATAAAAGTATTAAACATTAGTTTAAATCCAATAGACATTGAAAACATAAATAGTTTATTTACTTTTATTTATAAATTCAAAAATAATAATTTTTCTGCAAACGAATTGAATGACTTTTTAAGTTATTTATCTAATGAAATAAATTTTTTAAATTGCAAAATTCAATATTTTTTAATAATTAAAAATGTCGAATTTTCTTTAAGAAAAGACGTAAGTGAAAATTCAAACGCCCAAAATTTGTTTAATGAGTTGCAAAAACTTAAAGGTTATAATGATGATAAAAAAGCAATTGATTTAATATATGATTATATAGAAGAACAATACAACTAA", "aa": "MDIVEFIELKRWLESLLNLLKVQNKIINKIDEQEYQNNQIEFYIENFKRLTANIKVLNISLNPIDIENINSLFTFIYKFKNNNFSANELNDFLSYLSNEINFLNCKIQYFLIIKNVEFSLRKDVSENSNAQNLFNELQKLKGYNDDKKAIDLIYDYIEEQYN", "aa_hexdigest": "c03665429a8301de6a533d892b9062cf", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 501302, "stop": 502090, "observations": { "mutation": [ 501599 ], "stop": [ 501599 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D95B73", "query_cov": 1.0, "subject_cov": 0.42744063324538256, "identity": 0.975, "score": 305.0, "evalue": 1.87e-101, "gene_start": 1, "gene_end": 162, "reference_start": 218, "reference_end": 379, "reference_sequence": "MAGEHEHFPQHFIDNLKDKKTIIQKKLVDFNNDEYLKWNEKILKQNLLEIKNLIIEYATFYFKFKNNYKNLNYIVEKYKKNQQLIKELNNIKLEQLIKEILEKEFKFNYEIEVEKIKTLINKCQNSKDISNIEQIINKLLASINTTSIKIYNYIKTFAFHLKIQNNIKKIIEKIDIQNKKISDFFASKTKKNLLKSEYFNVYKPILNSFIDIKNNNWMDIVEFIELKRWLKSLLNLLKIQNKIINKIDEQEYQNNQIEFYIENFKRLTANIKVLNINLNPIDIENINSLFTFIYKFKNNNFSANELNDFLSYLSNEINFLNCKIQYFLIIKNVEFSLRKDVSENSNVQNLFNELQKLKGYNDDKKAIDLIYDYIEEQYN" }, "paralog": false, "identity": 0.9618320610687023, "score": 502.0, "evalue": 1.55e-181, "description": "Internal stop codon at 501599. Nonsense mutation around 501599.", "psc": { "uniref90_id": "UniRef90_UPI0034D95B73", "uniref50_id": "UniRef50_UPI0034D95B73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D95B73", "UniRef:UniRef50_UPI0034D95B73" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D95B73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D95B73" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19474.888499999997, "isoelectric_point": 4.803656578063964 }, "id": "AHCKFKLKGB_859", "locus": "GCA013367975_00855" }, { "type": "cds", "sequence": "contig_1", "start": 502101, "stop": 502199, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22025.1" ], "nt": "ATGGACTACAATAAAATTTTAATTAGATATGGTGAATTAACTCTTAAAGGAGAAAATAAAAAGGATTTTATTAGGCAATTGCATAACAACCTATCTTGA", "aa": "MDYNKILIRYGELTLKGENKKDFIRQLHNNLS", "aa_hexdigest": "fadac77c9c73137a76016e2ce1732acf", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22025.1", "name": "ThiI_fer", "length": 26, "aa_cov": 0.8125, "hmm_cov": 1.0, "evalue": 4.362885234797138e-12, "score": 41.67063522338867, "start": 6, "stop": 31 } ], "seq_stats": { "molecular_weight": 3865.4184000000005, "isoelectric_point": 9.301772499084471 }, "id": "AHCKFKLKGB_860", "locus": "GCA013367975_00856" }, { "type": "cds", "sequence": "contig_1", "start": 502428, "stop": 503249, "strand": "+", "frame": 3, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "SO:0001217", "UniRef:UniRef50_B3PLZ2", "UniRef:UniRef90_D1J8J1" ], "nt": "ATGACTTCAAGTGAACTAAATATGTATTTTGGAGGGGTTGTTTTAAAACAAATGCCAGATAAAGAAGTTAAATTAAAAAATCCTGATTTAACTATATATATTGAAATAAGAGATCAACGTAGCTTTGTTTTTTGCGATTATTTTGATGGCTTAGGTGGTATGCCGGTATTGAGTTCTGGAAAATGCATACATCTAATTAGTGGGGGCATTGACTCGCCTGTAGCAGCGAACTTATTACAAAAAAGAGGTTTAAAAATAGTGTTTTTAAATTTTATTACCCCTCCTCAAACAGATGAAAAAACAATTGCAAAAATAGATGAAATTGTTAATATATTAAAAAAATATCAAGGCAATGCTATGTTATATCAAATTAACTATAGCAAAGTAATGGAATATATTGGCCTTATAAGTAATCAAAAATATAAAATAACTTTAATGAGAAGATCTTTTTATAGAATAGCAAATCTTATTGCAAAAAAAGAAAACATTAAAGCAATTTCAAATGGCGAAAATTTAGCCCAAGTTGCATCTCAAACTCTTGAATCTATTTATACTATCTCCGAAGTTTGTGATTTGCCTATTTTTAGACCTTTAATTTCTTTTGACAAAAATGAAACTATTGCTATAGCAAAAAAAATTAACACATTAGATGTTTCAATAGTAAGGGCGTGTGAAACATGTGAATTATTTGCTCCTAAAAATCCAGTAACCAAGCCTAATAGGCAGGAAGCTCATAAACTCGAAGAAGAAGAATTACCTTTAATAAAAGAATTTGAAGAAGAATGTATTAAAAATGCTATTATAAAAAAATATTCAATATAA", "aa": "MTSSELNMYFGGVVLKQMPDKEVKLKNPDLTIYIEIRDQRSFVFCDYFDGLGGMPVLSSGKCIHLISGGIDSPVAANLLQKRGLKIVFLNFITPPQTDEKTIAKIDEIVNILKKYQGNAMLYQINYSKVMEYIGLISNQKYKITLMRRSFYRIANLIAKKENIKAISNGENLAQVASQTLESIYTISEVCDLPIFRPLISFDKNETIAIAKKINTLDVSIVRACETCELFAPKNPVTKPNRQEAHKLEEEELPLIKEFEEECIKNAIIKKYSI", "aa_hexdigest": "3d53dd9491921d0ad95ee415f5af8fee", "start_type": "ATG", "rbs_motif": null, "genes": [ "thiI" ], "truncated": "5-prime", "pseudogene": { "start": 502128, "stop": 503249, "observations": { "mutation": [ 502197 ], "stop": [ 502197 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8J1", "query_cov": 1.0, "subject_cov": 0.7146596858638743, "identity": 0.982, "score": 528.0, "evalue": 1.77e-187, "gene_start": 1, "gene_end": 273, "reference_start": 110, "reference_end": 382, "reference_sequence": "MDYNKILIRYGELTLKGENKKDFIRQLHNNLSWYIEKSEIKTEYDRAFVDYSQKNLDCLKYIFGISSYSPVYETETSLINIENIVKEIISKKQFDSFAINARRHNKNFSMTSSELNMYFGGIVLKQMPDKQVKLKNPDLTIYIEIRDQRSFVFCDYFDGLGGMPVLSSGKCIHLISGGIDSPVAANLLQKRGLKIVFLNFITPPQTDEKTIAKIDEIVNILKKYQGNAMLYQINYSKVMEYIGLISNQKYKITLMRRSFYRIANLIAKKENIKAISNGENLAQVASQTLESIYTISEVCDLPIFRPLISFDKNETIAIAKKINTLDVSIVRACETCELFAPKNPVTKPNRQEAYKLEEEELPLIKEFENECIENAIIKKYSI" }, "paralog": false, "identity": 0.9758713136729222, "score": 722.0, "evalue": 1.13e-266, "description": "Internal stop codon at 502197. Nonsense mutation around 502197.", "psc": { "uniref90_id": "UniRef90_D1J8J1", "gene": "ThiI", "product": "Probable tRNA sulfurtransferase", "uniref50_id": "UniRef50_B3PLZ2", "cog_id": "COG0301", "cog_category": "HJ", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8J1", "UniRef:UniRef50_B3PLZ2", "COG:COG0301", "COG:HJ" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLZ2" ], "product": "Probable tRNA sulfurtransferase" } }, "id": "AHCKFKLKGB_861", "locus": "GCA013367975_00857" }, { "type": "cds", "sequence": "contig_1", "start": 503302, "stop": 503415, "strand": "-", "frame": 1, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920J7V4" ], "nt": "ATGCCTCCAACAGGCGGTTCTGGTATAGGAATTGATCGTTTAATTATGCTATTAACTCAAAATGATTCAATTAGAGATGTAGTTTTATTCCCTCAATTAAAAGATTTAAAATAA", "aa": "MPPTGGSGIGIDRLIMLLTQNDSIRDVVLFPQLKDLK", "aa_hexdigest": "d9b52268f805181a0d249c6481f62c51", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A920J7V4", "query_cov": 0.8378378378378378, "subject_cov": 0.8611111111111112, "identity": 0.677, "score": 46.2, "evalue": 5.74e-06, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A920J7V4" }, "pscc": { "uniref50_id": "UniRef50_A0A920J7V4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920J7V4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_862", "locus": "GCA013367975_00858" }, { "type": "cds", "sequence": "contig_1", "start": 503443, "stop": 503940, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGGAAAGAACTGAAGGCGTTTTCAAACATTTAATAAATAAATTCAACATTAAAGAAGTTAAATTTAATGGCTTTGATATAACATTTAATTTTCCATTTAAGAGAGTTAATATGATTGATGCTATATCTGAAAAGATTAATGTTGACATTAGAAAATTAAATGAAGAACAAATGTTTGAATTAGCTAAAAAACATGGTATTAAAGTTGAAAAATTCTTTAGATATGGTCACATTATCAATGAATTATTTGAAAAATATGTTGAAGAAACATTAATAGAACCAACATTTGTATATGGCCACCCAATTGAAATTTCTCCTCTTGCAGCAATTGATCCTGACGATAAAAGATTTACACTACGTGCTGAACTATTTATTGGAACAAAAGAATTTGCAAATATGTTTACTGAATTAAATGATCCAATTGACCAATTGCAACGTTTTGAAAACCAATTGTTGGAAAAAGAAAATGGTAATGAAGAAGCAAATGAAATTGATTGA", "aa": "MERTEGVFKHLINKFNIKEVKFNGFDITFNFPFKRVNMIDAISEKINVDIRKLNEEQMFELAKKHGIKVEKFFRYGHIINELFEKYVEETLIEPTFVYGHPIEISPLAAIDPDDKRFTLRAELFIGTKEFANMFTELNDPIDQLQRFENQLLEKENGNEEANEID", "aa_hexdigest": "63d8fd55075fd618c782f9a6c8b2305e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0027D2801B", "query_cov": 0.9030303030303031, "subject_cov": 0.8097826086956522, "identity": 0.53, "score": 147.0, "evalue": 4.83e-42, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_A0AA38HFP6" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 165, "aa_cov": 0.9636363636363636, "hmm_cov": 0.806060606060606, "evalue": 6.8801703863284645e-15, "score": 50.169349670410156, "start": 1, "stop": 159 } ], "seq_stats": { "molecular_weight": 19551.041799999995, "isoelectric_point": 4.986791801452636 }, "id": "AHCKFKLKGB_863", "locus": "GCA013367975_00859" }, { "type": "cds", "sequence": "contig_1", "start": 503947, "stop": 504768, "strand": "-", "frame": 1, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EED3E5F" ], "nt": "ATGGAAAGAAAACTTAGTGAACAAGAAGAAATTAGACGTAATAAAATTGCTGAATTAGAAAAAAACAATTTACAAGCATTTCACAAAACTATAAAACCAACAGCAAATTCAAAAGAGTTAAATGAAAAATATAAAAATTTAAGTCGTGAAGATCTTGACAAACAAAATCTAGAATTTGTATTTAATGGAAGGATTATGACTATTCGTGGTCCATTTATTGTTTTACAAGATAATTGTGGACAAATGCAAATTTATGCAAACAAAAAAACATTGAATGAGTCTTCTGCAAAAACCTTTTCTTTAATGGATTTTGGCGATATTTTAAGTGTAAGAGGAACATTGTCAAAAACTAATACTGATGCTTTAGTTATCAAAGCTCAAGACATAGTTCTATTAACAAAATCATTGAAACCCTTACCTGATAAATTTCATGGTTTAGTTGATCCAGAAGAAAGACGTCGTCAACGCTATGTAGATTTAATAATGAATGAAAAAACAAGAAATACATTTGTTATGAGAACCCAAATTATCAAAGGCATTAGAGAATATTTTGATAGTTTGAACTACATTGAAGTTGATACACCAATTTTAAATCCAATATTAGGTGGTGCTGCTGCTAAACCTTTTATTACTTTCTATAATGCACTTAATAGAAACTTTTATTTAAGAATTGCAACTGAATTGCCATTAAAAAAATTGATTGTTGGTGGAATGGATAGAGTTTATGAAATCGGCAGAATATTTAGAAATGAAGGCGTAGATACAACTCATAATCCAGAATTTACCTCAATTGAATTTTATGAAGCGTATTCAGATTTATGA", "aa": "MERKLSEQEEIRRNKIAELEKNNLQAFHKTIKPTANSKELNEKYKNLSREDLDKQNLEFVFNGRIMTIRGPFIVLQDNCGQMQIYANKKTLNESSAKTFSLMDFGDILSVRGTLSKTNTDALVIKAQDIVLLTKSLKPLPDKFHGLVDPEERRRQRYVDLIMNEKTRNTFVMRTQIIKGIREYFDSLNYIEVDTPILNPILGGAAAKPFITFYNALNRNFYLRIATELPLKKLIVGGMDRVYEIGRIFRNEGVDTTHNPEFTSIEFYEAYSDL", "aa_hexdigest": "732c30408529c4bfa616675c4cdbc9d1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001EED3E5F", "query_cov": 0.9926739926739927, "subject_cov": 0.8885245901639345, "identity": 0.539, "score": 288.0, "evalue": 3.03e-94, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_UPI001EED3E5F", "kegg_orthology_id": "K04567" }, "pscc": { "uniref50_id": "UniRef50_UPI001EED3E5F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EED3E5F" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "AHCKFKLKGB_864", "locus": "GCA013367975_00860" }, { "type": "tRNA", "sequence": "contig_1", "start": 504860, "stop": 504934, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 68.0, "nt": "GGTCGCATAGCTCAGTGGAAGAGCACGAGCCTCCTAAGCCCGGGGTCGCAGGTTCAACTCCTGTTGCGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 504889, 504891 ], "id": "AHCKFKLKGB_23", "locus": "GCA013367975_00861" }, { "type": "cds", "sequence": "contig_1", "start": 505674, "stop": 505844, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAATTCTATACATATCTATGCTTTTATGTATTCCTGAAAGTTCAATCAATTCAATAGTAAATCTAGTTAACGAAATTACTATAAATATTGCTGGAATAATATATCAAGCCCATTTTATTTTAAAATCTTTTGCATTAGAAAAATTAAATAAATTTGGTAAAAAGTAA", "aa": "MVILYISMLLCIPESSINSIVNLVNEITINIAGIIYQAHFILKSFALEKLNKFGKK", "aa_hexdigest": "6944e64254b55f6c0519481cee3e0ed4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6310.574699999999, "isoelectric_point": 8.93223972320557 }, "id": "AHCKFKLKGB_865", "locus": "GCA013367975_00862" }, { "type": "cds", "sequence": "contig_1", "start": 505889, "stop": 506110, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001CC77350", "UniRef:UniRef90_UPI001CC77350" ], "nt": "ATGGATATTAAGCCCACAACAACAGGAAGTAATAACGATAGTAGTAAAAACATTGAAATTCCAACACGTACCTTTGAAAATCACATGGTAGAAGATTCAATGAATACTCAAAATTTGCAACAAATAAGTTACAAAATAAAAGATCCTCAGAAAATAATTCCTAATGGAATTTATTCAGTCTTTCAAAGAGAAATGAATATCAAAAAAATATTGATACTTTGA", "aa": "MDIKPTTTGSNNDSSKNIEIPTRTFENHMVEDSMNTQNLQQISYKIKDPQKIIPNGIYSVFQREMNIKKILIL", "aa_hexdigest": "2d7635d11943a8b5f9ab06eddcaa2ebb", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 505756, "stop": 506110, "observations": { "insertion": [ 505786 ], "mutation": [ 505891 ], "stop": [ 505891 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001CC77350", "query_cov": 1.0, "subject_cov": 0.6239316239316239, "identity": 0.9590000000000001, "score": 137.0, "evalue": 1.1e-40, "gene_start": 1, "gene_end": 73, "reference_start": 1, "reference_end": 73, "reference_sequence": "MDIKPTTTGSNSDSSKNIEIPTRTFENHMVEDSMNTQNLQQLSYKIKDPQKIIPNGIYSVFQREMNIKKISILWNFGILLLSVIAILLLYFWPNLFNFSNAKDFKIKWLDILFRQYL" }, "paralog": false, "identity": 0.9316239316239316, "score": 213.0, "evalue": 8.12e-73, "description": "Internal stop codon at 505891. Frameshift due to insertion around 505786. Nonsense mutation around 505891.", "psc": { "uniref90_id": "UniRef90_UPI001CC77350", "uniref50_id": "UniRef50_UPI001CC77350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001CC77350", "UniRef:UniRef50_UPI001CC77350" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001CC77350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001CC77350" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8454.601499999997, "isoelectric_point": 8.030133628845213 }, "id": "AHCKFKLKGB_866", "locus": "GCA013367975_00863" }, { "type": "cds", "sequence": "contig_1", "start": 506117, "stop": 506881, "strand": "-", "frame": 3, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003924", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q9ZHZ8", "UniRef:UniRef90_A0A454CA39" ], "nt": "ATGGATATTCTTCAAGAAAGACTCGAAAGAGAATTTAATTTAGATATTATTGCTACTGCTCCTTCAGTTGAGTTTGTTGTAACACTAACTAATGGGGATGTTCAATATATTACAAATCCATCACTATTTCCAGACAGAAGTTATATAAAAATGATAGAGGAACCTTATATTAAGGCTAATATAATGTTAACCGAAGAATATTTGGGCCCAATTATGGAATTATGCCAATCTAAGCGTGGTAAATATGTAAATATTGAATATTTAGATGAAACTCGCAGATTATTGATTTATGAATTGCCTTTAAATGAAATAATATTTGATTTCTTTGATTTAATGAAGAGCTATTCAAAAGGCTATGCTTCATTTGATTATGACTACATTGGTTTAAGAGAAAGCGATTTAGTAAAGGTTGATATTTTATTAAATGGTGAGAAAATTGATGCCTTAGCAATGATTGTCCATAAAGACTCGGCATATAATAAAAGTAGAGCATTAACAGAAAAATTAAAGGAAGTGGTACCAAGACAAAATTTTGAAATTCCCGTTCAAGCTGCAATTGGTGCTAAAATTATAGCAAGAGAAACAATTAAGGCATATAGAAAAGATGTTACAGCTAAGTTATATGGTGGTGATGTTACAAGAAGACAAAAACTATTAAAAAAACAAAAAATAGGTAAAAAAAGGATGAAACAAATAGGAAGCGTTGAAGTCCCACAAGAAGCATTTTTGGCAATATTAAAAACAGATGATTCTCGAAAGGATTAG", "aa": "MDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHKDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD", "aa_hexdigest": "8f107582d8e501a141d9db8374051d14", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449BBE2", "query_cov": 1.0, "subject_cov": 0.8639455782312925, "identity": 0.685, "score": 343.0, "evalue": 2.85e-116, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A449BBE2" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 506117, "stop": 507181, "observations": { "mutation": [ 506947, 507154 ], "stop": [ 506947, 507154 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA39", "query_cov": 1.0, "subject_cov": 0.4240400667779633, "identity": 0.996, "score": 488.0, "evalue": 8.87e-169, "gene_start": 1, "gene_end": 254, "reference_start": 346, "reference_end": 599, "reference_sequence": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDTSDAIMISAKTGLNIDKVIHAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTEKHYLVTELGVKNPNEVQKNELQAGEVGWISASIRDIKDVEVGDTVTLFENQCPEPLPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLVWQPETSKALGFGFRIGFLGLLHMDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHRDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD" }, "paralog": false, "identity": 0.9915254237288136, "score": 686.0, "evalue": 2.14e-249, "description": "Internal stop codon at 506947, 507154. Nonsense mutation around 506947, 507154.", "psc": { "uniref90_id": "UniRef90_A0A454CA39", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q9ZHZ8", "go_ids": [ "GO:0003924", "GO:0005525" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA39", "EC:3.6.5.n1", "UniRef:UniRef50_Q9ZHZ8", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZHZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZHZ8" ], "product": "Elongation factor 4" } }, "id": "AHCKFKLKGB_867", "locus": "GCA013367975_00864" }, { "type": "cds", "sequence": "contig_1", "start": 506945, "stop": 507070, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAGGATATAAAAAAATAAAACCAGTTGTTTATACTGGATTTTATCCTGTCGATACTAGAGATTACAATGATTTAAAAGATGCCCTAGAAAAAATTAGTTTATCTGATTCATCGTTGGTATGA", "aa": "MPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLV", "aa_hexdigest": "4a49afffe937ca97bc817e164d8b878b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4667.291399999999, "isoelectric_point": 5.986134910583496 }, "id": "AHCKFKLKGB_868", "locus": "GCA013367975_00865" }, { "type": "cds", "sequence": "contig_1", "start": 507152, "stop": 507916, "strand": "-", "frame": 3, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003924", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q9ZHZ8", "UniRef:UniRef90_A0A454CA39" ], "nt": "ATGACAAACGAAAAAATAAGAAATTTTGCAATAATAGCACACATTGATCACGGTAAAAGTACTTTAGCTGATCGTATTTTGGAATTTACCAATACAATTTCTGCCAGGGAAGCAAAACCTCAAATTTTAGATTCAATGGAACTAGAACAAGAACGTGGAATCACTATTAAATTAAATGCTATTCAAATTAAATATAAAGATTACATTTTTCACCTGATCGATACACCCGGTCATGTTGATTTTACTTATGAAGTTAGTAGATCACTTGCTGCATGTGAGGGAGCATTATTGCTAGTTGATGCAACTCAAGGAATTCAAGCACAAACATTGGCTAATGTTTATATGGCAATGGAAAATAATTTAGAAATTGTTCCTATTATTAATAAGGTAGATTTAATTAGTGCAGATCCCGAAAGAGTAAAAAAAGAAATTCAAGACATGATTGGAATTGATGCTTCTGATGCAATTATGATTTCAGCAAAGACAGGATTGAATATTGATAAAGTAATTGATGCAATTATAAACAAAATCCCCGCTCCTAAAGAAGCAGATATAAATAAACCATTGCAAGCTTTAATTTTTGATAGCTATTTTGACAATTATCGTGGCGTTGTTATCTATGTGAGATTGTTTGATGGCAAAGTAAAAGTAGGCGACGAAATTTACTTTATGCAAACAGAAAAGCATTATTTAGTAACGGAACTAGGCGTTAAAAATCCTAATGAAGTTCAAAAAAATGAATTGCAAGCTGGCGAAGTTGGTTGA", "aa": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDASDAIMISAKTGLNIDKVIDAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTEKHYLVTELGVKNPNEVQKNELQAGEVG", "aa_hexdigest": "c30ab95eaf3fd539c9a8970ed0e6cc76", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00317F3865", "query_cov": 1.0078740157480315, "subject_cov": 0.8827586206896552, "identity": 0.633, "score": 325.0, "evalue": 4.9e-109, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A0P1NWJ7" }, "genes": [ "lepA" ], "truncated": "5-prime", "pseudogene": { "start": 506849, "stop": 507916, "observations": { "mutation": [ 506947, 507154 ], "stop": [ 506947, 507154 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA39", "query_cov": 1.0, "subject_cov": 0.4240400667779633, "identity": 0.992, "score": 489.0, "evalue": 2.2e-169, "gene_start": 1, "gene_end": 254, "reference_start": 1, "reference_end": 254, "reference_sequence": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDTSDAIMISAKTGLNIDKVIHAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTEKHYLVTELGVKNPNEVQKNELQAGEVGWISASIRDIKDVEVGDTVTLFENQCPEPLPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLVWQPETSKALGFGFRIGFLGLLHMDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHRDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD" }, "paralog": false, "identity": 0.9887323943661972, "score": 685.0, "evalue": 6.12e-249, "description": "Internal stop codon at 506947, 507154. Nonsense mutation around 506947, 507154.", "psc": { "uniref90_id": "UniRef90_A0A454CA39", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q9ZHZ8", "go_ids": [ "GO:0003924", "GO:0005525" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA39", "EC:3.6.5.n1", "UniRef:UniRef50_Q9ZHZ8", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZHZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZHZ8" ], "product": "Elongation factor 4" } }, "id": "AHCKFKLKGB_869", "locus": "GCA013367975_00866" }, { "type": "cds", "sequence": "contig_1", "start": 508018, "stop": 509214, "strand": "-", "frame": 1, "gene": null, "product": "endopeptidase La", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9X8" ], "nt": "TTGGGCGGTGTTAAGGATGAAAGCGAAATAAGAGGACACAGAAGAACTTATGTTGGGGCAATGCCTGGAAAAATAATATCAGCAATTAAAAAAGCCGGAGTTTCAAACCCAGTCATTTTGTTAGATGAAATTGACAAGATGTCATCAGATTTTCGAGGCGATCCAACAAGCGCAATGCTTGAAGTATTAGACCCTGAACAAAATGTTAATTTTCAAGATCACTACTTGGATTTAGAATACGACTTATCAAAAGTATTATTCATCGCTACTGCTAACTACTATGATTCAATACCCGCAGCACTAATTGATCGTGTTGAATTACTTGAACTATCAACATATACAACTAAAGAAAAAGTAGAAATTGCTAAATCACATTTACTTCCAAAAGTGTATGAACAAAATGCCTTAAATCCTGAACAATTTCAAATTTCTGACGAAGTTTTAGAATTTTTAGTTCGTTACTACACAAGGGAAAGTGGAGTTCGTGAATTAAAACGTATTTTAGATAGTATAGCAAGAAAGATTGTTGTCGAAATTCTTGACAAAAAAATCAACGATAGTTTTGTTGTTACAAAAGAAGTTGTAATTGAATTTTTAGGACCAATTAAATTCGATGATGATGAAAATGAAAATAAGGCACAAATAGGTGTTGTTAATGGTTTGGCATATACAAGTTTTGGTGGTTCAACATTAGCTATTGAAGTTACAACATTCCCTGGAAAAGAAGGCCTAAAAATGACAGGGCAACTAAAAGATGTTATGCGTGAATCTGCTCAAATTGCATTAGCTTATGTAAGAGCAAATGCTGCCAAATTTAATATTGACTTTGATTTTGAAAATACAGCAATTCATATTCACGTTCCAGCAGGTGCTATTCCAAAAGATGGCCCAAGTGCCGGAGTAACATTCACAACCTCAATAATTAGTGCCTTATCTAAAAGGCCAGTCCCTGCTAATATCGGTATGACTGGAGAAATTACATTGCGTGGAAAAGTTCTACCAATTGGCGGTTTAAAAGAAAAATCATTGGCAGCTGCTCAATTTGGTATTAAAAAGATTTTTATTCCATATGATAATTATAAAAATCTAAAAGATGTTCCTGCTGAAGTTAAAAATGTTGTTGAATTTGTACCAGTTCATTATTATGATGAAATATTTGAACAAATATTTGGTGCCAAGGAAACAAAAAATAATTAA", "aa": "MGGVKDESEIRGHRRTYVGAMPGKIISAIKKAGVSNPVILLDEIDKMSSDFRGDPTSAMLEVLDPEQNVNFQDHYLDLEYDLSKVLFIATANYYDSIPAALIDRVELLELSTYTTKEKVEIAKSHLLPKVYEQNALNPEQFQISDEVLEFLVRYYTRESGVRELKRILDSIARKIVVEILDKKINDSFVVTKEVVIEFLGPIKFDDDENENKAQIGVVNGLAYTSFGGSTLAIEVTTFPGKEGLKMTGQLKDVMRESAQIALAYVRANAAKFNIDFDFENTAIHIHVPAGAIPKDGPSAGVTFTTSIISALSKRPVPANIGMTGEITLRGKVLPIGGLKEKSLAAAQFGIKKIFIPYDNYKNLKDVPAEVKNVVEFVPVHYYDEIFEQIFGAKETKNN", "aa_hexdigest": "b0980c4df91b123b45b1a67b1dc795d6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A432F9X8", "query_cov": 0.9824120603015075, "subject_cov": 0.8593406593406593, "identity": 0.547, "score": 435.0, "evalue": 8.42e-148, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A432F9X8", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432F9X8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9X8" ], "product": "endopeptidase La" }, "genes": [], "id": "AHCKFKLKGB_870", "locus": "GCA013367975_00867" }, { "type": "cds", "sequence": "contig_1", "start": 509554, "stop": 509952, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAGTACTTTTTGAGTGATGAACTTCTTTCTTTAATTGAAAGCAATGACGTTTTAGCTAGATACAATGAACTAATTAAAAAGGCAAATAAACAAGTTCAATTTGAAAAAATAGAAAACGAAATTAATAGTAATTTACGTGAAGATATGGAAGACCAACAAAGAGATTTCTTCCTAAGAGAAAAAATGCGTCAAATTAGAAAAATGTTGAAGGATGATGAAGGTGGAAAATCTGCTGACGACATTGAAAAAGACAGTACTTTAAAAGAACAATATCCTGAATATGTTTTAGAAGCATTAAGATCTGAACAATCAAGAATGGCTTCAATGATGCCATCTAGCCCAGAAGCTAATATATCTAAAACATACATCGATTTGATCCTTGCATTGCCTTGA", "aa": "MKKYFLSDELLSLIESNDVLARYNELIKKANKQVQFEKIENEINSNLREDMEDQQRDFFLREKMRQIRKMLKDDEGGKSADDIEKDSTLKEQYPEYVLEALRSEQSRMASMMPSSPEANISKTYIDLILALP", "aa_hexdigest": "8caae9d413dc55a00fb0ae39141fdaf3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15544.501499999995, "isoelectric_point": 4.859586143493652 }, "id": "AHCKFKLKGB_871", "locus": "GCA013367975_00868" }, { "type": "cds", "sequence": "contig_1", "start": 509968, "stop": 510501, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTACCTTATATAGCAATAAAAAATCAGATTATTATTCCATATACTTCTGACAAAATTAGAGTTGGAAAAGCTAAATCAGTGTCAGCTTTATTAAAGGCTTTTAATGGAAATAAAAATATAGTTATTACATTTTTAAATCCAAATTATAAACAATCAAATGAGGAACATACAATTAATGATATTGCTCCTGTAGGCGTACTTGTTAATATTACTAACATTAGTGAAGAATCAGGAATTTATCATGTTAATTTAGAAGCAAAAGAACCGGTTAATATTAATAAAATCTCAATAGTTGACGAAAAATCATCAAATAGTGAGGATAATTTTTATGTTAATATTTCGCCTATTCAAGTCAATTCATATGATTCATATTTACCAAAAATTGAAGAAATAAGACAAAAGTTCTTCTCACTATCTAAACAAGTTATTAACGAAGGTATAGGAACAGTTTTATTTCGAGATTTAGAAAAAGAATTTGAAACTAACAAACCTAAACAATCATTAGTTGAATATGAAACTGAATCATGA", "aa": "MKLPYIAIKNQIIIPYTSDKIRVGKAKSVSALLKAFNGNKNIVITFLNPNYKQSNEEHTINDIAPVGVLVNITNISEESGIYHVNLEAKEPVNINKISIVDEKSSNSEDNFYVNISPIQVNSYDSYLPKIEEIRQKFFSLSKQVINEGIGTVLFRDLEKEFETNKPKQSLVEYETES", "aa_hexdigest": "eca0b95c2ef2d19deb6479c8d0350fac", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20084.538300000007, "isoelectric_point": 5.518350028991698 }, "id": "AHCKFKLKGB_872", "locus": "GCA013367975_00869" }, { "type": "cds", "sequence": "contig_1", "start": 510568, "stop": 511356, "strand": "-", "frame": 1, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "KEGG:K03687", "RefSeq:WP_175933549.1", "SO:0001217", "UniParc:UPI00158D8823", "UniRef:UniRef100_UPI00158D8823", "UniRef:UniRef50_D1J8J6", "UniRef:UniRef90_D1J8J6" ], "nt": "ATGAGTCAAAATATTTCAAAATATGATTATATAGAGTGTATTAGAAAAATTAACAAAAACGAAAAAATTGAATATAAGGGATTTGTTGGCTTTGATTTAGATAAAAATATTGAAAAAACTATCTTGGAAGGAAAGCAAAACGAAAAAGGTGAGCTAGTTTTAAAAATTGATGATGAAATTTTTAAAGTAATAATTATTGATTTTCAAAAGACAAGTCCTAAGTATTTAGAAGTTTTTGCTAAAAATCAAGAATTAAATGAAAATATTAAGAAATTGCAACTAAATTTTTTGGAATTAGGCGAACTTAATGAAAAAATAAAACAAGAAAAGACACAACAAGAAATTTTATTTAAAAATCAAGTAATTGAATTGGAAGCAAAAGCACAATCAAAAATAAATGAACATCGTCAAAAAAATGATGAGCATTTATTGCAACAAAAAACAGAATTAAAAAAATATGCCCTACAAGACTTTTTAGAAGAGTTCATTAAGATATATACAAAATATGACAGTGCTTTAAATTTTGCAAAAAAATCTGATAATATCGCTGTTAATAATTTTGCAAAAGGCTTTGATATGCTTAAAAATGATTTTGAAAATTTAATGTTAGATAACGGAATAAAAATAATTGAACCAAAAGTAGGAGATTTATTTGACCCTGAATGTCAACAAATTACTGAAAGCATCGAATCAAAAGAACCCTCTGGCACAATTCTTGAAGTAAAGAGCAATGGTTATTCATTATTTAACAGAATTTTAAAACCCGCCTCAGTAATAATTAGTAAATAA", "aa": "MSQNISKYDYIECIRKINKNEKIEYKGFVGFDLDKNIEKTILEGKQNEKGELVLKIDDEIFKVIIIDFQKTSPKYLEVFAKNQELNENIKKLQLNFLELGELNEKIKQEKTQQEILFKNQVIELEAKAQSKINEHRQKNDEHLLQQKTELKKYALQDFLEEFIKIYTKYDSALNFAKKSDNIAVNNFAKGFDMLKNDFENLMLDNGIKIIEPKVGDLFDPECQQITESIESKEPSGTILEVKSNGYSLFNRILKPASVIISK", "aa_hexdigest": "7d3d6e57c5695e2d976fb9eeab7a1be1", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158D8823", "ncbi_nrp_id": "WP_175933549.1", "uniref100_id": "UniRef100_UPI00158D8823", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D8823", "RefSeq:WP_175933549.1", "UniRef:UniRef100_UPI00158D8823" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D8823", "uniref90_id": "UniRef90_D1J8J6" }, "psc": { "uniref90_id": "UniRef90_D1J8J6", "gene": "GrpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_D1J8J6", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687" }, "pscc": { "uniref50_id": "UniRef50_D1J8J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J6" ], "product": "Protein GrpE" }, "genes": [ "grpE" ], "id": "AHCKFKLKGB_873", "locus": "GCA013367975_00870" }, { "type": "cds", "sequence": "contig_1", "start": 511576, "stop": 512409, "strand": "-", "frame": 1, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "SO:0001217", "UniRef:UniRef50_B3PN01", "UniRef:UniRef90_D1J8J7" ], "nt": "ATGGAGCAAAATTCTTCTGACACAAACACAAATAAGTCTTTAATCATATGCAATAATTATGATAAAAAACTAATTAAATATTTTAGATACATTGTTGAAATGTACATTAAAACAGGCGAACCAGTAGGATCAAAAAGATTAGTTGAAAAATTTAAATTATCTTGTAGCTCTGCAACTATAAGAAACGCAATGGCAGAATTAGAAAAACTAGGTCTTTTAGAAAAAAAACATGTTTCAGGAGGAAGAATTCCTTCTTCAAAAGGTCTTGAATACTATACAAAAAATTTGCTTTACAATGCCAAAAAATATTTCAACGAAAAACTCGAAGATCTTTTATCTAAGCGTAGGTTAAGCATCAACACAACATTAGAAGAAGCAGCAAATGTCGTTAGCCAAATGGCCGGCTTTGCCGTAGTTGCAACTTCAAACAATGAATCTGAATTATTGAAAAGTATTCAGTTGACACCGCTTGATGAAGAATCTGCAGTTGTTGTTATTGTTACAAGTTCAGGAAATGTTCAAAACAAAATGTTTAAGTTTGAAAGTGGGATTGAATTAAATGATTTAAGGATTGCAATTAGATTATTTAAAGAAAGATTGATTGATACTCCGTTAATTAATCTTGCTAAAAAAACTGAATCATTAGCCCCAATATTATCTACACAAGTTAAACATTTTGAATTAATAATGCAGAAATTTATCAAAAATATTTTTTGTTTTGAAGAAAAATTGGAGAATAAATCGTTTAATAAAAATGTAATAATATTATCAGAACAAATAAGCAGAGAAGAAATCGCTAAGACATTAGAATTAATTGAAAACCACTCAGTTTGA", "aa": "MEQNSSDTNTNKSLIICNNYDKKLIKYFRYIVEMYIKTGEPVGSKRLVEKFKLSCSSATIRNAMAELEKLGLLEKKHVSGGRIPSSKGLEYYTKNLLYNAKKYFNEKLEDLLSKRRLSINTTLEEAANVVSQMAGFAVVATSNNESELLKSIQLTPLDEESAVVVIVTSSGNVQNKMFKFESGIELNDLRIAIRLFKERLIDTPLINLAKKTESLAPILSTQVKHFELIMQKFIKNIFCFEEKLENKSFNKNVIILSEQISREEIAKTLELIENHSV", "aa_hexdigest": "109813c048a62b0c73f606ed405225b7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 511357, "stop": 512409, "observations": { "mutation": [ 511578 ], "stop": [ 511578 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8J7", "query_cov": 1.0, "subject_cov": 0.7914285714285715, "identity": 1.0, "score": 522.0, "evalue": 1.26e-185, "gene_start": 1, "gene_end": 277, "reference_start": 1, "reference_end": 277, "reference_sequence": "MEQNSSDTNTNKSLIICNNYDKKLIKYFRYIVEMYIKTGEPVGSKRLVEKFKLSCSSATIRNAMAELEKLGLLEKKHVSGGRIPSSKGLEYYTKNLLYNAKKYFNEKLEDLLSKRRLSINTTLEEAANVVSQMAGFAVVATSNNESELLKSIQLTPLDEESAVVVIVTSSGNVQNKMFKFESGIELNDLRIAIRLFKERLIDTPLINLAKKTESLAPILSTQVKHFELIMQKFIKNIFCFEEKLENKSFNKNVIILSEQISREEIAKTLELIENHSVWEAIENNLDEEKNIKLDFSHPNLNIISKRIQFINHKNIKEISILGPKNLDVEESLEAINLFEGIIKKEEEKGE" }, "paralog": false, "identity": 0.9971428571428571, "score": 669.0, "evalue": 4.1e-246, "description": "Internal stop codon at 511578. Nonsense mutation around 511578.", "psc": { "uniref90_id": "UniRef90_D1J8J7", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_B3PN01", "cog_id": "COG1420", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8J7", "UniRef:UniRef50_B3PN01", "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN01" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "AHCKFKLKGB_874", "locus": "GCA013367975_00871" }, { "type": "tRNA", "sequence": "contig_1", "start": 512536, "stop": 512612, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(acg)", "amino_acid": "Arg", "anti_codon": "acg", "score": 76.1, "nt": "GCGCCCGTAGCTCAATTGGACAGAGTGTTTGGTTACGGCCCAAAAGGTTGCGGGTTCGACTCCTGCCGGGCGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 512576, 512578 ], "id": "AHCKFKLKGB_25", "locus": "GCA013367975_00872" }, { "type": "tRNA", "sequence": "contig_1", "start": 512615, "stop": 512689, "strand": "-", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 67.3, "nt": "GCCACCATAGCCAAACGGCCAAGGCATAGGTCTGCAACACCTTGATTACCGGTTCGAATCCGGTTGGTGGCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 512664, 512666 ], "id": "AHCKFKLKGB_24", "locus": "GCA013367975_00873" }, { "type": "cds", "sequence": "contig_1", "start": 512891, "stop": 513412, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J8", "UniRef:UniRef90_D1J8J8" ], "nt": "TTGAATACAAACCAATCATTAGAAAATTTTGCACCTGAATTAAAAAATGAAATCAAAGATATTACTTCAATGAAATCAAATCAGGGATTATTGCTTATTGATCAAAATCTTGTTAATGCAAGAAATATTCTAAAATTTTTAGCCTTCTTATTTGGCACTAAGAAAAACAAAAGCGTAGCATTTGTTGACAACAATAATTTGTTTGAATTCATGTCTAATAATTTAAAAAATTCATCAGAAATTAATTCAATTGTTCAAAACTTAATTTCTGTTGATTATTTATTCATAGATCGCTTTGCAATCGGAGTCAAACCGGAATGGTATATTACAAACATATTTAATATATTGAGCGAACGTGAACAAAAAAATAAAATCACTTTTATGAGCTCGCCTATAGATTTTACTTCTTCAAAGCAACATATAATATTTAATAAAATGCCTGGCAATGACGGCATAACTCGGATTGAATCATTGCTTAAATTGACAGTCTTAAGATTAATGAAAAAGTTCATTTCATTCTAA", "aa": "MNTNQSLENFAPELKNEIKDITSMKSNQGLLLIDQNLVNARNILKFLAFLFGTKKNKSVAFVDNNNLFEFMSNNLKNSSEINSIVQNLISVDYLFIDRFAIGVKPEWYITNIFNILSEREQKNKITFMSSPIDFTSSKQHIIFNKMPGNDGITRIESLLKLTVLRLMKKFISF", "aa_hexdigest": "5016187b76dba128f59bc68de8169264", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 512891, "stop": 513712, "observations": { "mutation": [ 513463, 513496 ], "stop": [ 513463, 513496 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8J8", "query_cov": 1.0, "subject_cov": 0.5766666666666667, "identity": 0.983, "score": 328.0, "evalue": 1.7e-111, "gene_start": 1, "gene_end": 173, "reference_start": 128, "reference_end": 300, "reference_sequence": "MENKIFDELFKNSLLKNIDLEAEKAKILADPNVKAIIEKLNLNSKQIQKGMNYLQKYYSYIETHKQLPIWTLFVDDNGILQIDLSNDGFFRKQKEMNNFWLTNITSMDNEWIYYFNNAKAKKPQYIVLNTNQSLEKFAPELKNEIKDITSMKSNQGLLLIDQNLVNARNILKFLAFLFGTKKNKSVAFVDNNNLFEVMSNNLKNSSEINSIVQNLISVDYLFIDRFAIGVKPEWYITNIFNILSEREQKNKITFMSSPIDFTSSKQHIIFNKMPGNDGITRIESLLKLTVLRLMKKFISF" }, "paralog": false, "identity": 0.978021978021978, "score": 525.0, "evalue": 1.43e-191, "description": "Internal stop codon at 513463, 513496. Nonsense mutation around 513463, 513496.", "psc": { "uniref90_id": "UniRef90_D1J8J8", "uniref50_id": "UniRef50_D1J8J8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8J8", "UniRef:UniRef50_D1J8J8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8J8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19965.930000000004, "isoelectric_point": 9.454498100280762 }, "id": "AHCKFKLKGB_875", "locus": "GCA013367975_00874" }, { "type": "cds", "sequence": "contig_1", "start": 513494, "stop": 513793, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAAAATATTTGATGAATTGTTTAAAAATTCTCCTTTGAAAAATATTGATTTAGAGGCTGAAAAAGCAAAAATTCTAGCCGATCCAAATGTAAAAGCAATTATTGAAAAATTGAATTTGAATTCTAAACAAATTCAAAAAGGAATGAATTACTTGCAAAAATATTATAGTTATATTGAAATGCATAAACAACTTCCAACATGGACTCTCTTTGTTGATGATAACGGAATTTTGCAAATTGATTTGAGCAACGATGGTTTTTTTAGAAAGCAAAAAGAAATGAATAACTTTTGA", "aa": "MENKIFDELFKNSPLKNIDLEAEKAKILADPNVKAIIEKLNLNSKQIQKGMNYLQKYYSYIEMHKQLPTWTLFVDDNGILQIDLSNDGFFRKQKEMNNF", "aa_hexdigest": "3201eff376288a8438ba2507279fa9c6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11716.365399999991, "isoelectric_point": 6.571974372863769 }, "id": "AHCKFKLKGB_876", "locus": "GCA013367975_00875" }, { "type": "cds", "sequence": "contig_1", "start": 513822, "stop": 514718, "strand": "-", "frame": 2, "gene": "dnaB2", "product": "DnaD domain-containing protein", "db_xrefs": [ "COG:COG3611", "COG:L", "KEGG:K03346", "SO:0001217", "UniRef:UniRef50_D1J8J9", "UniRef:UniRef90_D1J8J9" ], "nt": "ATGGTTGAAAATTTTTTTATTGAAAGCGAAAGGGAAATTACACAAAATGATTTTAAAAATCTAGTAATTTTTTATGCTCCGATTTTAGGCCATAGTAGCATATTTCTATATCAATTTTTATATAATTTAAAAAATATTTATACAAAAAATAAACTTTTTAATTTTTCTGAAATTAAAGATATTTTATTATTAAATGAACAAGAATTTGAGGAATGTCGTAGTAAACTAGAAGCGCTAAGCTTACTAAAAAGTTATTTAAACAAGGATGGTGTTTATATTTTTAGTTTAAATGCTCCATTGAATGCAAATGAAATAAGCAACAATAAAATTATTTCTAATATGTTAATTAAATTAATTACTAAATCAAGATATGAAGAATTATTAAAATTAAATGCAAATTATCAATTTGATAAAACAACAATTAATGATGTTTCTAAAAAATTTTATGAAGTATTTAATCTAGAAAACGTAATTGATTTAAATATAAAAAATGAACAATTAAATTTTAATTCAATTACCCTAGATGAATTAAAGGATAAGATAGAACCCGAACAATTTATTAGTCAATATACAAAAACAAATTTAAGTTTTAGCCAAAATGAAATGATTAGAAAATTGAAAAGAATGAAATTCAATAATTTTTCAATAAATGCCTTCATTTATTATTCAATTAAAATTAACCATTCAGTTGTATGCAAATATATTGAAAAAATAGCGGAAGATTTTAGCCATAGAAATCTATACGATGCCGAAAGAATTGATCAAGAATTAGAAAATGTTTATCTAATAAAGCAAAATGCAAATAAAGAGTTAAAAAATATCAAAAAAAATGAAGATGAATCAGTTACTAGAGAAGATTTAAATTGTTTTTTAGATCTTGCCAACGAACATGATTGA", "aa": "MVENFFIESEREITQNDFKNLVIFYAPILGHSSIFLYQFLYNLKNIYTKNKLFNFSEIKDILLLNEQEFEECRSKLEALSLLKSYLNKDGVYIFSLNAPLNANEISNNKIISNMLIKLITKSRYEELLKLNANYQFDKTTINDVSKKFYEVFNLENVIDLNIKNEQLNFNSITLDELKDKIEPEQFISQYTKTNLSFSQNEMIRKLKRMKFNNFSINAFIYYSIKINHSVVCKYIEKIAEDFSHRNLYDAERIDQELENVYLIKQNANKELKNIKKNEDESVTREDLNCFLDLANEHD", "aa_hexdigest": "d6ddf8720c988a0e54b2f18afdca50ca", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8J9", "query_cov": 1.0, "subject_cov": 0.9900332225913622, "identity": 1.0, "score": 570.0, "evalue": 4.38e-205, "valid": true, "gene": "DnaB2", "product": "DnaD domain-containing protein", "uniref50_id": "UniRef50_D1J8J9", "cog_id": "COG3611", "cog_category": "L", "kegg_orthology_id": "K03346" }, "pscc": { "uniref50_id": "UniRef50_D1J8J9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J9" ], "product": "DnaD domain-containing protein" }, "genes": [ "dnaB2", "dnaD" ], "id": "AHCKFKLKGB_877", "locus": "GCA013367975_00876" }, { "type": "cds", "sequence": "contig_1", "start": 515033, "stop": 515383, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAATTAACCTTCACTTAAACACTATATTTTCTTTTTTAGAAAGCACGATAACCATTAATGATTTTATTCAAGAATTGTTAAAGAATAATATAAAATATTTTGGCATTACAGAACATTGTAATTTTTATAGCATGCCATATTTTTTAAATGAGGCTAGAAAATATGATTTAAAACCTATTTTTGGTCTTGACGTAAATGTAAAAATAGAAAATAATTTGTATAGATTTATAGTTTACTGCCAAAATAAAGAATCTTTTATGAACCTAAAAATGCTTTCTTTAAACTACGGAAAAAAAGGCTATATAGATTTAGCTGAAATTAGAGATTATTCTAATTTAATATGA", "aa": "MKLINLHLNTIFSFLESTITINDFIQELLKNNIKYFGITEHCNFYSMPYFLNEARKYDLKPIFGLDVNVKIENNLYRFIVYCQNKESFMNLKMLSLNYGKKGYIDLAEIRDYSNLI", "aa_hexdigest": "0a2f7c5eb9a78a0aa39a3e76a507c870", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13856.973199999984, "isoelectric_point": 7.721883964538574 }, "id": "AHCKFKLKGB_878", "locus": "GCA013367975_00877" }, { "type": "cds", "sequence": "contig_1", "start": 515567, "stop": 515824, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAATATTTCAAATAATAAAATATTTGAAAGTATTTACGAAGAATTTAAAGTTGATATTGAAACAAAAAATAATTCAAAATTAGAAGAATTAATTGAAAGAACAAATAATTTTGCTAAAAAACTTTATTTTGAAATTGAAACTAATGGTTTTAAAATGCCCAAATTTAAAAACGACAAAAATATTGCTTCTAATGAATATTTAAAATTGTTATTATTTAAATCAATAAGAGAAAAATTTGATAAGTTTTCATGA", "aa": "MSNISNNKIFESIYEEFKVDIETKNNSKLEELIERTNNFAKKLYFEIETNGFKMPKFKNDKNIASNEYLKLLLFKSIREKFDKFS", "aa_hexdigest": "217951195377aefb0cc3c80414cd76ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10227.589599999988, "isoelectric_point": 8.802658271789554 }, "id": "AHCKFKLKGB_879", "locus": "GCA013367975_00878" }, { "type": "cds", "sequence": "contig_1", "start": 516068, "stop": 518023, "strand": "+", "frame": 2, "gene": null, "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023586AB7" ], "nt": "ATGCCTGACATTGATATTGATGTTCAAGATAATAAAAGAAATGATGTTATTGAATATTTATTAAAAAAATATGGGCATGAATTCGTGGCAAATATTGTTACTTTTTCTTCACTAGGTAAAAAATCAGCGATTAGAGATATTTTAAGAATAAACAATATTGCCCCAACATTAATTAATGAAATATCAAAAAATATTGAAAATGATGAACAAGACCTGTTAGTTGAATACAATGAAAATAGAAATTTACAATTATCATTAGCAAAATTAAATCCTAATGATGAATCTTTTAGCACAAGAATATTAAACCAAGCAGCAAGGATATCAGGATTTTATAGACAAATTGGCACACATGCTGCCGGATTAGTAATTAGCGACCAACCCGTCATCTCAATAGCACCAGTTCAATATAATTCTGAAAATTTTCTTCAAACTCAAATAAGCATGGAATATTTAGAAGATTTCGGGTTAGTTAAAATGGACATACTTGGTTTGAAGACATTGACGACTATTTCAAATATAATTGATCTAATTTATCAAAATCAAAACCTTAAAATTGACTTAAAAGATATAGACTTAACCGATCAAAAAACATTAGAGTTATTGAGCTCTGGCCACACAAGAGGCGTTTTCCAAGTTGAAAGTCCAATCATGATAAAAGCCTTAAGACAGGTAAAGGTAAATAATTTTGAAGATATTGTTGCAATAATTTCATTAAATAGACCGGGTCCAATGAAAAACGTTTCGGTTTATGCAAAAAGAAAATTCGGACTAGAGCCGATACCAAAAATAAATAATGAATATGATAAAATCCTTGATTCTACTTATGGAATCATTGTATATCAAGAACAAATTATGAAAATTGCTCAAGTTGTTGCTAAAATGACATTCATTGAAGCCGACAATCTAAGAAGGATTATTTCTAAGAAAAAAGTAAATGAAATGGAAGATATTAAATCTATTTTTATTGAAAGGTCAATGAAAAATGGCTATGAAAAGTCTTTGGCTACAAATATCTTTAACAGCATAGAAAAATTTGCATCATATGGATTTAATAGATCTCATGCGGTTTCATATTCAATTATAACTTTTCAAATGGCCTTTTTAAAAGCTCACTACCCCTTAGAATTTTATACATCATGTATAAGCTCGGCACACGGCTCCCAAGATACAATTGCAAAGTTCGTTAACGAGGCAAGTGAGTGTGGGGTAGAAATAGTTTCTCCTGACATTAACCTTTCTGAATCAGAAGCTATAATTAAAAATAAAAAAATTATATTGCCACTTACACTAATTAAAGGTCTTGGGCCTGAAATTGCTAATTCAATTGTTGAAAATAGATCAAAATATGGCCCATATAAAAATTTCCTAGATTTCTTATTAAAAATTTCTAGCTCTCAATCAATTGGCAAAAGTACAATTGAATTATTAATAAAGGCAAACACTCTTAGATCATTTAATTATAATCAAAACACATTGTTGAATGAGATTCAAAAAAACAATACTGATACAATGTTATTTATAAAAATGTTTAAGAATAACATTGAAGGTGTTAGCTCTAATATAATAAACTCTTATAAACCAAGTGAAATTCTTAATCAAGATATCATGTTTGAAAAGAAAAACGAAATTGAATTATTAGGTCAAGTTTATAATTTTAATTCAAGCGCAAGCAATGGAATGCCTGGACAAACTAGTTTTATAGACATGCACGTTGGAAATGAATATTTAGCAATCGTTAACTGTACAAACGTAAAGCAAAAAATTTCTAAAAAAGGTAATAACTATTTTGAATTAGATTTAGAAGCAAACATGCAAAAAATAAGAGCATTTTATTTTACATCAAATCAAGAAATACTAAATGTTAAGGGAAAAACTATCAAAGTAACTCTAGAAAAAAGAAATGAAAATATTTTTTATTTGCGTTCAATAAAGGAAGTTTTAAATGAAGAAAGATAA", "aa": "MPDIDIDVQDNKRNDVIEYLLKKYGHEFVANIVTFSSLGKKSAIRDILRINNIAPTLINEISKNIENDEQDLLVEYNENRNLQLSLAKLNPNDESFSTRILNQAARISGFYRQIGTHAAGLVISDQPVISIAPVQYNSENFLQTQISMEYLEDFGLVKMDILGLKTLTTISNIIDLIYQNQNLKIDLKDIDLTDQKTLELLSSGHTRGVFQVESPIMIKALRQVKVNNFEDIVAIISLNRPGPMKNVSVYAKRKFGLEPIPKINNEYDKILDSTYGIIVYQEQIMKIAQVVAKMTFIEADNLRRIISKKKVNEMEDIKSIFIERSMKNGYEKSLATNIFNSIEKFASYGFNRSHAVSYSIITFQMAFLKAHYPLEFYTSCISSAHGSQDTIAKFVNEASECGVEIVSPDINLSESEAIIKNKKIILPLTLIKGLGPEIANSIVENRSKYGPYKNFLDFLLKISSSQSIGKSTIELLIKANTLRSFNYNQNTLLNEIQKNNTDTMLFIKMFKNNIEGVSSNIINSYKPSEILNQDIMFEKKNEIELLGQVYNFNSSASNGMPGQTSFIDMHVGNEYLAIVNCTNVKQKISKKGNNYFELDLEANMQKIRAFYFTSNQEILNVKGKTIKVTLEKRNENIFYLRSIKEVLNEER", "aa_hexdigest": "e08b70882e1511d19ee186e280b4eaab", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0023586AB7", "query_cov": 0.9815668202764977, "subject_cov": 0.8753424657534247, "identity": 0.504, "score": 587.0, "evalue": 2.55e-199, "valid": false, "product": "DNA polymerase III subunit alpha", "uniref50_id": "UniRef50_UPI0023586AB7" }, "pscc": { "uniref50_id": "UniRef50_UPI0023586AB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023586AB7" ], "product": "DNA polymerase III subunit alpha" }, "genes": [], "id": "AHCKFKLKGB_880", "locus": "GCA013367975_00879" }, { "type": "cds", "sequence": "contig_1", "start": 518010, "stop": 518903, "strand": "+", "frame": 3, "gene": "exoIX", "product": "DNA polymerase I", "db_xrefs": [ "COG:COG0258", "COG:L", "RefSeq:WP_073673945.1", "SO:0001217", "UniParc:UPI00093CB3A5", "UniRef:UniRef100_UPI00093CB3A5", "UniRef:UniRef50_A0A318U4A1", "UniRef:UniRef90_D1J8K1" ], "nt": "ATGAAGAAAGATAATGAACAACTGTTGATTATAGATGGTACATTTTTAGCATACAGATCTTATTATGCAACATCTTATAACGCAACTCAAATTTTAACCACATCTGAAGGTTTTGAAACTAATGCGATAGTAGCATTCTTTAAAACACTTTTTTTATTACTAAAAAAATTAAATCCTAAATATATTTATATAGCCTTTGATTCGCATGTTAAGACGTTTAGGCATGAATTATTTCCTTCTTATAAAGATGGTAGAAAAAAAGCACCAGAAAGTTTTTATCTACAACTTGATTTAATTCAGGAAATATTAACAAATATTAATATTCAAAACCAATATTATAATGGCTTTGAGGCTGATGATATAATTGCAAAAGTTAAAAATAAATTTAATTTGAATACAATCATTTTTTCAGGAGATCAAGATTTAAATCAACTGATAGATGAGCAAACGGCAATCATTAAAAAAGATAAAACTGGTTCTTTCTTTTTTCTTAATTCAAAGAATTTTTCAGAATATTATGATTTTTTGCCAAATCAAGTTATTGATTATAAGGCATTAGTCGGTGATAACAGTGATAATTTTAAGGGTGTTGTAGGAATTGGACCTAAATCTGCTAAAACTTTATTAGAAGATTATAAGACTATTGAAAATATATACAATAATATTGAAAAAATAAAACCTTCAATTGCCAAAAAATTAATGGAATATAAAGAAAATACGTTTCTCGATAAATATTTAGCAACCTTAAGGATAGACTTTGATTTGCAACTTCCAAAATTAGAAAATTTAGCAATATCTAATATTGCAATTTCAAACAGTGCAAAACAAATTATTGACAAATTTGAACTATATTCTCTGGAAAATTCTTTGAAGAATTTAATCAATAAAAAATAA", "aa": "MKKDNEQLLIIDGTFLAYRSYYATSYNATQILTTSEGFETNAIVAFFKTLFLLLKKLNPKYIYIAFDSHVKTFRHELFPSYKDGRKKAPESFYLQLDLIQEILTNINIQNQYYNGFEADDIIAKVKNKFNLNTIIFSGDQDLNQLIDEQTAIIKKDKTGSFFFLNSKNFSEYYDFLPNQVIDYKALVGDNSDNFKGVVGIGPKSAKTLLEDYKTIENIYNNIEKIKPSIAKKLMEYKENTFLDKYLATLRIDFDLQLPKLENLAISNIAISNSAKQIIDKFELYSLENSLKNLINKK", "aa_hexdigest": "216bf676e24c5a02efe842a653bce933", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00093CB3A5", "ncbi_nrp_id": "WP_073673945.1", "uniref100_id": "UniRef100_UPI00093CB3A5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00093CB3A5", "RefSeq:WP_073673945.1", "UniRef:UniRef100_UPI00093CB3A5" ] }, "ips": { "uniref100_id": "UniRef100_UPI00093CB3A5", "uniref90_id": "UniRef90_D1J8K1" }, "psc": { "uniref90_id": "UniRef90_D1J8K1", "gene": "ExoIX", "product": "DNA polymerase I", "uniref50_id": "UniRef50_A0A318U4A1", "cog_id": "COG0258", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_A0A318U4A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318U4A1" ], "product": "5'-3' exonuclease, exo domain of DNA polymerase I" }, "genes": [ "exoIX" ], "id": "AHCKFKLKGB_881", "locus": "GCA013367975_00880" }, { "type": "cds", "sequence": "contig_1", "start": 518930, "stop": 519301, "strand": "-", "frame": 3, "gene": "cof", "product": "HAD family phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_A0A318UA29", "UniRef:UniRef90_D1J8K2" ], "nt": "TTGCCAGCAACAACAAAAATTTTAACTTTTGGGAAGTCAAAAAAGGGAATAATAAAACTTAGAGATGAATTATTAAATGCTTTTCATGATATTTCAACACATATTGTATCAAAAGGATATTCAATTGAAATTAGCGATATTAATGCCAATAAAGGAATTGGCGATCGATTTGTATGCGATTTGTTAAATATTGATTATAAAGAAGCAGTTCACTTAGGCGATTCGGGTAATGATGTTGTTACTCAAAAATATTTAGGTGGCTTTGTAGCAATGAAAAATTCCACAAAGAACATAAAGCAAAAAGCAGATTATGTAGGATATTCATATAAAAAATCTGGAGTAGCAAAAACGTTGCAAGAACTTCAAAAATAA", "aa": "MPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTQKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK", "aa_hexdigest": "e2821e4ba0226feb7075948f6be44d6e", "start_type": "TTG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 518930, "stop": 519601, "observations": { "mutation": [ 519346 ], "stop": [ 519346 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K2", "query_cov": 1.0, "subject_cov": 0.458955223880597, "identity": 0.9840000000000001, "score": 240.0, "evalue": 3.49e-78, "gene_start": 1, "gene_end": 123, "reference_start": 146, "reference_end": 268, "reference_sequence": "MINFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARAWLKKLNKVTYDEIQSLPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTKKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK" }, "paralog": false, "identity": 0.9910313901345291, "score": 435.0, "evalue": 1.34e-157, "description": "Internal stop codon at 519346. Nonsense mutation around 519346.", "psc": { "uniref90_id": "UniRef90_D1J8K2", "gene": "Cof", "product": "HAD family phosphatase", "uniref50_id": "UniRef50_A0A318UA29", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K2", "UniRef:UniRef50_A0A318UA29", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318UA29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318UA29" ], "product": "COF family HAD hydrolase protein" } }, "id": "AHCKFKLKGB_882", "locus": "GCA013367975_00881" }, { "type": "cds", "sequence": "contig_1", "start": 519344, "stop": 519736, "strand": "-", "frame": 3, "gene": "cof", "product": "HAD family phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_A0A318UA29", "UniRef:UniRef90_D1J8K2" ], "nt": "ATGATTAATTTTAAACCTAAGGCATATTTTTTGGATATGGATGGCACATATCTAGATTTGCCTCGCAAAAAACAAATTATGATAAGCGAAAAAAATGTTGAGGTTGCTAAGGACTTCAACAATAGAGGAATCCCTGTTATTTTGTCAACAGGTAGAGGAAATTCAAAGTTTGTTTTGGATTTAACTAATAAAATAAATGCACCGTTTGCTGTTTGCCAAAATGGTGGAGTAATCGTAGATAAAAATAATAATGTTTTGAAACGCCATGAAATTAATCCTGATACATTGAAATCAATAATTGAAATTTTAATTGCACAAAAAATGTTTTTTATTTTTAATAGTGGCGATACTATATATGGAACAAAAACAAAATTTAGAATTGCTAGAGCTTGA", "aa": "MINFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARA", "aa_hexdigest": "8c27f040cf025701d81bd45bddccd7be", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "cof" ], "truncated": "5-prime", "pseudogene": { "start": 519041, "stop": 519736, "observations": { "mutation": [ 519346 ], "stop": [ 519346 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K2", "query_cov": 1.0, "subject_cov": 0.48507462686567165, "identity": 1.0, "score": 261.0, "evalue": 1.44e-86, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MINFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARAWLKKLNKVTYDEIQSLPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTKKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK" }, "paralog": false, "identity": 0.9913419913419913, "score": 454.0, "evalue": 4.29e-165, "description": "Internal stop codon at 519346. Nonsense mutation around 519346.", "psc": { "uniref90_id": "UniRef90_D1J8K2", "gene": "Cof", "product": "HAD family phosphatase", "uniref50_id": "UniRef50_A0A318UA29", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K2", "UniRef:UniRef50_A0A318UA29", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318UA29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318UA29" ], "product": "COF family HAD hydrolase protein" } }, "id": "AHCKFKLKGB_883", "locus": "GCA013367975_00882" }, { "type": "cds", "sequence": "contig_1", "start": 519744, "stop": 519950, "strand": "-", "frame": 2, "gene": null, "product": "Thioredoxin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P2T3" ], "nt": "ATGATTGGCCCAGAATTAGAAAAATTAGCAGAAGAAAATTCAGATGTTAATGTTTTTGATTTGGACGTAGATAAATTCATTGATTTAGCAAGAGAAATGAATGTATCTCATATTCCATCGTTATTTTATTATAGAGACGGTAAGTTAGTTTCTCAAAGTGTAGGATACTTGCCAGTTGAAGAAATTTTAAACAATTTAAATAAGTAG", "aa": "MIGPELEKLAEENSDVNVFDLDVDKFIDLAREMNVSHIPSLFYYRDGKLVSQSVGYLPVEEILNNLNK", "aa_hexdigest": "b8d7bd8624b1522d9c36828a90a78435", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P2T3", "query_cov": 0.8382352941176471, "subject_cov": 0.9193548387096774, "identity": 0.544, "score": 72.8, "evalue": 1.09e-15, "valid": false, "product": "Thioredoxin", "uniref50_id": "UniRef50_A0A3B0P2T3" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P2T3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P2T3" ], "product": "Thioredoxin" }, "genes": [], "id": "AHCKFKLKGB_884", "locus": "GCA013367975_00883" }, { "type": "cds", "sequence": "contig_1", "start": 519966, "stop": 520130, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATACTTGTTTTTGAACTAATAGAAAAACAAAAAGATTTGTATTATTTGCAAGAGCATATTAAAGGAGAAAATATTATGTATAAAACTATTGAATCAGTTGAAGAATTCAAAAAGAATGAATATTTAGAAGGAAAATCAATTGTTGCATTTAGAGCAGTTTGA", "aa": "MILVFELIEKQKDLYYLQEHIKGENIMYKTIESVEEFKKNEYLEGKSIVAFRAV", "aa_hexdigest": "e3c34a065d1b30a44f763fd2991c67c8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6499.5041, "isoelectric_point": 5.297132682800293 }, "id": "AHCKFKLKGB_885", "locus": "GCA013367975_00884" }, { "type": "cds", "sequence": "contig_1", "start": 520114, "stop": 520236, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09180.16" ], "nt": "GTGTATGCTGCATTTGATGGCAATGGTGATGATGAAGTAAAAATTAAAGAAGAAACAGGAGCATCAACAAGGTGTATTCCATTTAAAGAACCAATAAAAGTTTCAAATGATACTTGTTTTTGA", "aa": "MYAAFDGNGDDEVKIKEETGASTRCIPFKEPIKVSNDTCF", "aa_hexdigest": "79c1090abc86c7c2c3a94b405e703492", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09180.16", "name": "ProRS-C_1", "length": 40, "aa_cov": 0.95, "hmm_cov": 1.0, "evalue": 5.844353755546745e-11, "score": 37.764034271240234, "start": 3, "stop": 40 } ], "seq_stats": { "molecular_weight": 4427.896500000001, "isoelectric_point": 4.507070732116699 }, "id": "AHCKFKLKGB_886", "locus": "GCA013367975_00885" }, { "type": "cds", "sequence": "contig_1", "start": 520237, "stop": 520689, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGACTCATGGTGATGATCGAGGAATAATCATTCCACCTAAAATAGCAAGAACTCAAGTTGATATATTAGAAATTTTTAATTCAAAGGATGAACGAGTTCACAAACTTGCCCTTGAATTAAAAGATGCTTTATTAGCAAAAAATATTGTTGCAAACATAGATTCTTCAAACAAAGGTCCAGGTTTTAAAGCAGCAAATTCAGAAATTCATGGTACACCATTGAGAATAGAAATCGGTCCAAAGGATATTGAAAATAATAGTGTATTATTCGTTAGACGTGATAATCTAGAAAAAATTACTTCAACAATTGCTGAAGCAGCAAATATTGCTAGTCTCTTATTGCAAAAAATACAAGACAATTTATTTGAAAGTGCTAAACAACGCTTACAAAATAATATTGTAACTGTCAATAGTTATCAAGAATTTAAGGAAGCAATTGCTGCGGGTCACTGA", "aa": "MTHGDDRGIIIPPKIARTQVDILEIFNSKDERVHKLALELKDALLAKNIVANIDSSNKGPGFKAANSEIHGTPLRIEIGPKDIENNSVLFVRRDNLEKITSTIAEAANIASLLLQKIQDNLFESAKQRLQNNIVTVNSYQEFKEAIAAGH", "aa_hexdigest": "2974e7188416bfeff6ac0b25bc5a6f88", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 82, "aa_cov": 0.5466666666666666, "hmm_cov": 1.0, "evalue": 8.079155283305965e-16, "score": 53.357421875, "start": 27, "stop": 108 } ], "seq_stats": { "molecular_weight": 16592.710699999985, "isoelectric_point": 6.423908805847168 }, "id": "AHCKFKLKGB_887", "locus": "GCA013367975_00886" }, { "type": "cds", "sequence": "contig_1", "start": 520723, "stop": 520968, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACAAGGTTTATAAAGATGTTATTGAAGAAAATTTAGCAATTCCAGTAGTAGAAGGCTGCAAAACGGATCATGAAAAATTTGCTGGCGCTGATATAACATACACCTTAGAATCAATGATGAAGGACGGAAAGGCACTTCAATCAGGTACTAGCCACTATTTAGGACAAAATTTTACTAAGGCATTTGATATAACATTTAAAAACAATGAAAATAAATTAATTAATCCTTTTGGTACCAGTTGA", "aa": "MHKVYKDVIEENLAIPVVEGCKTDHEKFAGADITYTLESMMKDGKALQSGTSHYLGQNFTKAFDITFKNNENKLINPFGTS", "aa_hexdigest": "9f9f35b3fc6881aaeb1e7cdccceaac90", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9072.162599999992, "isoelectric_point": 5.837330436706543 }, "id": "AHCKFKLKGB_888", "locus": "GCA013367975_00887" }, { "type": "cds", "sequence": "contig_1", "start": 521089, "stop": 521340, "strand": "-", "frame": 1, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "nt": "ATGGATATTGAATTTAAAAAGAGAGGCGTTCAAAACGTTTATTTACCTCTTTTAATACCAGAAAGCTTATTGCAAAAAGAAAAAAATCATGTTGAAGGATTTAACCCTGAACTTGCAACTGTTACTGAAGTAGGCGGCAAAAAATTGGCAGAAAAGTTTTTTATAAGACCAACTTCAGAAACGTTGTTTGCTGATTTATTCAAAAATGAAGTTTCATCTTACAATGATTTGCCAATAATTTATAATCAATGA", "aa": "MDIEFKKRGVQNVYLPLLIPESLLQKEKNHVEGFNPELATVTEVGGKKLAEKFFIRPTSETLFADLFKNEVSSYNDLPIIYNQ", "aa_hexdigest": "be7654839c9a3e753250bfb8135032dd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A661HMY4", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.614, "score": 102.0, "evalue": 4.5e-27, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A661HMY4" }, "pscc": { "uniref50_id": "UniRef50_A0A661HMY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_889", "locus": "GCA013367975_00888" }, { "type": "cds", "sequence": "contig_1", "start": 521559, "stop": 521954, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTGCAATTGACATTGCTTCATCAAATACCAAAAACCAATTTGTATTTGTAAATCTTGTTCAATTTGACAGCCATTTCGGACATAGAGAAGACCCAATAGGATATTCACAAAATATTTCAAGGTTAGATATTAAATTAACAAAATTGATAAATGCAATGAAATCAGATGACTTGTTAATTATAACTTCTGACCATGGAAATGACCCAACAAAAGGCCCTGATCATACAAGAGAAGCATTGCCATTGACAATATTTTCAAAGAAATTCAAATCACCATGTGTATTTAAAGAACCTTTGAAAGGCTTGGGAACTGCAGGAAATATTGTTGCAAGAAATTTCAATGTTCCAACCGTTCAAACAGGTGAAGATATTTTTGATAAATTAATCTAA", "aa": "MDVAIDIASSNTKNQFVFVNLVQFDSHFGHREDPIGYSQNISRLDIKLTKLINAMKSDDLLIITSDHGNDPTKGPDHTREALPLTIFSKKFKSPCVFKEPLKGLGTAGNIVARNFNVPTVQTGEDIFDKLI", "aa_hexdigest": "efe568986058dda60c7d493c057c6d40", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14543.415699999978, "isoelectric_point": 6.397819709777832 }, "id": "AHCKFKLKGB_890", "locus": "GCA013367975_00889" }, { "type": "cds", "sequence": "contig_1", "start": 522174, "stop": 522476, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGAATTTATACAAAAGTTCCAAACCCACAATTCATTGAAAAAGGTTTCCCTGCTGATTTAGTTGCAGAATTATCAAAAGCATTTGATAATAGAAAAATTATTGGAAATAGAAATGCATCAGGTACAGTTATATTGGCCGAATTAGGTTTGCAACACATTGAAAAAGGAGACATGATAATTTACACTTCTCCTGATTCAACATTGCAAATTATTGGGGATGAAAAAACATTGGGTGTTGAAAAACTAACTGAATATGCAAAGAGAGCAAGAGCAATTTGTTCATCAAGACCTGAATGA", "aa": "MMGIYTKVPNPQFIEKGFPADLVAELSKAFDNRKIIGNRNASGTVILAELGLQHIEKGDMIIYTSPDSTLQIIGDEKTLGVEKLTEYAKRARAICSSRPE", "aa_hexdigest": "5d61518090ed1c2deb07abf8c68a2cfe", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11024.595599999986, "isoelectric_point": 6.585217857360839 }, "id": "AHCKFKLKGB_891", "locus": "GCA013367975_00890" }, { "type": "cds", "sequence": "contig_1", "start": 522606, "stop": 522755, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAACAAAAATTTAATAGAATATTTTTTATAGTTACAGATGGTCTTGGCATTGGATATGAACCACGCCAAGCTGAATTTCATGACGAAGGTGCAAATAGCATGTTGCATGCGTCAGAAGCATTGCCACTAGAAATTCCTACATGA", "aa": "MQEQKFNRIFFIVTDGLGIGYEPRQAEFHDEGANSMLHASEALPLEIPT", "aa_hexdigest": "4eec1535153f3fde8ad1d1f95641cfd4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5549.166199999999, "isoelectric_point": 4.583291816711426 }, "id": "AHCKFKLKGB_892", "locus": "GCA013367975_00891" }, { "type": "cds", "sequence": "contig_1", "start": 523047, "stop": 524297, "strand": "+", "frame": 3, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_L0RUB4", "UniRef:UniRef90_D1J8K6" ], "nt": "ATGAAATTTAATGAACTAAGAACTAAAATTGTTGCAACAATTGGCCCTTCTTCAGATAATTATGACACAATGAAGAGTTTAATTGAAGCCGGAGTTACTACAATTAGAGCCAATTTTTCACATGGCGGTCCTGAAACTCATGTTAATAAATTTGAAATAGCAAGACAAATTTCAAAAGAACTAAACGTCCCAATCTCATTAATGCTTGACACAAAAGGCCCTGAAATTAGAGTTGGAAAAATGGAAAATGATGGAGTTACAATCCCTGCAAATCACATTTTAAAAATTAAAACAGATGAAAATTCATACAAGAATTTTTTAGGCACCCAAGATCAAATTACTGTTTCATATAGAATGGACAACGATCTTAGAATAGGGGATAAAGTTTTAATTGATGATGGAAAATTAGTTACAACTGTAAAAGATATTAAAAAATTCGAAATTATTGTTGAAACAGTTAATGAACATTTCTTAAAAACTAATAAAAGAATTAACATTCCTGGTGTTAACTTCTCACTTCCTTTTTTAAGTCAAAAAGATATTGACGATATTATATGGGGTGCGAAATACAAAGTTGATTATATTGCAGCATCATTTGTAAATTCTGCTAAAAATGTAAAAGAAATTAGAGACATATTAAACGCAAATGGTGGAGAAAATATTCAAATCATTTCAAAAATTGAATCTAAGATGGCAATCAATAACATTGATGAAATAATTGAAGCTTCAGACGGAATTATGGTAGCAAGAGGTGACCTTGGACTTGAAATTCCTTACTATGATGTTCCTTACTACGAAAAGAAAATAATTCGTAAATGTAGATTAGTAGGCAAGGAAGTTATAGTTGCAACTCAAATGCTAGATTCAATGGAAAACAACCCTATTCCAACAAGAGCGGAAGTTACTGACGTTTATTATGCAGTTGAATTAGGTGCAGACTCAACAATGTTATCTGGAGAATCTGCAAGTGGTAAATACCCTGTTGAAGCAGTTAAAGTTATGTCTAAAATTGCAAAAAGAGCAGAAAAAGAATATTACAATGAATTATTCTATGGCAAACAAATAGAAAAAGTAGCAAATCATCCTACTTCAAGAATGCTAATTGCTTATGAAGTTGCCAAAATCCTTCAAAGCGGTGAATACAAATTTGCCATTATATTAAGTCACACCGGAAAACTATTAAAAGAAGTTGCTAAATATAGACCAAACGCATTTATTGTTGGAGTTGTTAGCGACAATAGATTGGTTTGA", "aa": "MKFNELRTKIVATIGPSSDNYDTMKSLIEAGVTTIRANFSHGGPETHVNKFEIARQISKELNVPISLMLDTKGPEIRVGKMENDGVTIPANHILKIKTDENSYKNFLGTQDQITVSYRMDNDLRIGDKVLIDDGKLVTTVKDIKKFEIIVETVNEHFLKTNKRINIPGVNFSLPFLSQKDIDDIIWGAKYKVDYIAASFVNSAKNVKEIRDILNANGGENIQIISKIESKMAINNIDEIIEASDGIMVARGDLGLEIPYYDVPYYEKKIIRKCRLVGKEVIVATQMLDSMENNPIPTRAEVTDVYYAVELGADSTMLSGESASGKYPVEAVKVMSKIAKRAEKEYYNELFYGKQIEKVANHPTSRMLIAYEVAKILQSGEYKFAIILSHTGKLLKEVAKYRPNAFIVGVVSDNRLV", "aa_hexdigest": "21d04a8271f54140635de8e76e0bc84c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8K6", "query_cov": 1.0, "subject_cov": 0.879492600422833, "identity": 0.99, "score": 807.0, "evalue": 5.99e-294, "valid": true, "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_L0RUB4", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_L0RUB4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RUB4" ], "product": "Pyruvate kinase" }, "genes": [ "pyk" ], "id": "AHCKFKLKGB_893", "locus": "GCA013367975_00892" }, { "type": "cds", "sequence": "contig_1", "start": 524662, "stop": 525381, "strand": "-", "frame": 1, "gene": null, "product": "Restriction endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q098", "UniRef:UniRef50_UPI001E622C03", "UniRef:UniRef90_A0A6A8Q098", "UniRef:UniRef90_UPI001E622C03" ], "nt": "ATGTGTAAATTGGGTTTATATAATAGCAATTCAAAAACGATTTTAAAATCTGAATTGAATAATTTTTTTAATTTAGAAAATTTGAAATATTTAAATGAAGACAATTTTTATTCATTGCCAATTTTTAAGGTGGATAATATTGAAAAAAATATCAAGTTTCCATTTAAATATGAAGAAGTAATAGATATTTTAAATCAAATTCAAAGTGAAAAATTTGAATTTAAAGAAGCACCATCAGAAAAATACATTGCCCTAAAACACGTGCCAAATTTTGCGTTAGCAGAATATTTTGTTAATTTATTCTTCACATATTCCCTTAATATTAATCCGCAAAATTTTAAAAGTATATGTCATACAAAATTAACAAAGAATTTGTATCCAATTTTTTCTGCTCCCGGACATTCGGCAGATTTCGATTATTATGATTCAAAAAACAACATTCAGTATATAGTTGAAACGACTATACTAAAAACAGAGAAGGAAGTTGTTGATGGTGAAAATTATTCAATATCAAAACATGCACAAAACCTAGTTAACGCAAATCAAAATATAAAGGAAATTAATTTATATTTTGTAAATTTTTTAAAATTTAACGAAGAACAAAAAAAAGAATTAGAATCCGTATTTTTATCTACATATAAATCAATGCTTGAAAAAAATAATGTTGGGAAAAATATTTATATAAAAAAATTCTCTGAATTAATTAATTTAAATTTATAA", "aa": "MCKLGLYNSNSKTILKSELNNFFNLENLKYLNEDNFYSLPIFKVDNIEKNIKFPFKYEEVIDILNQIQSEKFEFKEAPSEKYIALKHVPNFALAEYFVNLFFTYSLNINPQNFKSICHTKLTKNLYPIFSAPGHSADFDYYDSKNNIQYIVETTILKTEKEVVDGENYSISKHAQNLVNANQNIKEINLYFVNFLKFNEEQKKELESVFLSTYKSMLEKNNVGKNIYIKKFSELINLNL", "aa_hexdigest": "f9087b7c503f52d6e2681de2618060fe", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E622C03", "query_cov": 0.8117154811715481, "subject_cov": 0.8899082568807339, "identity": 0.995, "score": 382.0, "evalue": 3.31e-133, "valid": true, "uniref50_id": "UniRef50_UPI001E622C03" }, "pscc": { "uniref50_id": "UniRef50_UPI001E622C03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E622C03" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 524662, "stop": 525681, "observations": { "mutation": [ 525384 ], "stop": [ 525384 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8Q098", "query_cov": 1.0, "subject_cov": 0.45610687022900764, "identity": 0.996, "score": 465.0, "evalue": 2.6e-161, "gene_start": 1, "gene_end": 239, "reference_start": 286, "reference_end": 524, "reference_sequence": "MKKQGFNKFGGTEFRVAEPINEYFSILSWVNYERISIENNLSNNLQKNFNVNWDEEFKKYVKNQKIYNYSNDKKIKSRFNFVISHLKKWGFLKNSKNLVFNFEPKKFFDTDSPVEELFFKKMYLYWEEFREMVNFIISCNESSISIEKLNWAFAVFDYKEDKFEDLYAKNKQQILEEIADVSNITNIKSLFKKKINEYKILEKIFNLLRIKEPLSYSDYLELKPKEFKYIKRLLNIPPKERNVNYLDKINFYIFNHLQEDFLLDLKKISIEFNIEKEYMDMFNRWMCKLGLYNSNSKTILKSELNNFFNLENLKYLNEANFYSLPIFKVDNIEKNIKFPFKYEEVIDILNQIQSEKFEFKEAPSEKYIALKHVPNFALAEYFVNLFFTYSLNINPQNFKSICHTKLTKNLYPIFSAPGHSADFDYYDSKNNIQYIVETTILKTEKEVVDGENYSISKHAQNLVNANQNIKEINLYFVNFLKFNEEQKKELESVFLSTYKSMLEKNNVGKNIYIKKFSELINLNL" }, "paralog": false, "identity": 0.9941002949852508, "score": 675.0, "evalue": 2.96e-246, "description": "Internal stop codon at 525384. Nonsense mutation around 525384.", "psc": { "uniref90_id": "UniRef90_A0A6A8Q098", "product": "Restriction endonuclease", "uniref50_id": "UniRef50_A0A6A8Q098", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8Q098", "UniRef:UniRef50_A0A6A8Q098" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q098", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q098" ], "product": "Restriction endonuclease" } }, "id": "AHCKFKLKGB_894", "locus": "GCA013367975_00893" }, { "type": "cds", "sequence": "contig_1", "start": 525382, "stop": 525618, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAGAATCAAAGAACCTTTGTCATATAGTGATTATTTAGAATTGAAACCCAAAGAATTTAAATATATAAAAAGATTATTAAATATTCCCCCAAAAGAAAGAAATGTAAACTATTTAGATAAAATAAATTTTTACATATTTAATCATTTACAAGAAGATTTTTTGCTAGATTTAAAAAAAATCTCTATTGAATTTAATATCGAAAAAGAATATATGGACATGTTCAATAGATGA", "aa": "MLRIKEPLSYSDYLELKPKEFKYIKRLLNIPPKERNVNYLDKINFYIFNHLQEDFLLDLKKISIEFNIEKEYMDMFNR", "aa_hexdigest": "26b7e4e233c6063d320d028564fd0e27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9751.304899999996, "isoelectric_point": 7.901583671569825 }, "id": "AHCKFKLKGB_895", "locus": "GCA013367975_00894" }, { "type": "cds", "sequence": "contig_1", "start": 526463, "stop": 526888, "strand": "-", "frame": 3, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "EC:2.1.1.72", "KEGG:K06223", "SO:0001217", "UniRef:UniRef50_A0A6A8PYM9", "UniRef:UniRef90_A0A6A8PYM9" ], "nt": "ATGATATATATATATATATATATATATAAGAAGTATGGCTCAATTGATAAATTAGATCCCGTAACGAAAAGAGTAAATTCATCAATTTATAATTCGATAAAAAGTGAATATAATTTATCAAAAAATAAGTATTCATATGAATCTTGTATAGTTTTATTAGTTCTAATAATTTATGGATTTAATAGTCAAATAAGATTTAATTCAAAAGGAGAATTTAATATTCCTTCCGGAAAACAAATTTTAAATGAAACAAGGAAACAATGCCTTGTCGATTTTTCTTCAACTTTAAATAGAAAAAATATTGATTTTTCTTCATTGGATTTTCAAGAATTTATATATTTGAAAATGCAAGAAACAAGAGAAGACGATTTTTGGTATTTTGATCCTCCATACTTAATTTCGGGTGCAATATATAATCAGGCCTGA", "aa": "MIYIYIYIYKKYGSIDKLDPVTKRVNSSIYNSIKSEYNLSKNKYSYESCIVLLVLIIYGFNSQIRFNSKGEFNIPSGKQILNETRKQCLVDFSSTLNRKNIDFSSLDFQEFIYLKMQETREDDFWYFDPPYLISGAIYNQA", "aa_hexdigest": "9a9c905308e20e14d284c478182ff584", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 526217, "stop": 526940, "observations": { "insertion": [ 526880 ], "stop": [ 526399, 526465 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8PYM9", "query_cov": 0.9787234042553191, "subject_cov": 0.5774058577405857, "identity": 1.0, "score": 279.0, "evalue": 1.23e-93, "gene_start": 4, "gene_end": 141, "reference_start": 20, "reference_end": 157, "reference_sequence": "MCNIVKLLSNTNPIKILNDIYIYIYKKYGSIDKLDPVTKRVNSSIYNSIKSEYNLSKNKYSYESCIVLLVLIIYGFNSQIRFNSKGEFNIPSGKQILNETRKQCLVDFSSTLNRKNIDFSSLDFQEFIYLKMQETREDDFWYFDPPYLISGAIYNQAWTLEEDRKLFKLLDLLDSKNIKWGLSNLFQSKGKSNTELIEWSKKYKVFHLNKQYTNSNYQRKNRDQLDEEVYVTNYEKAGI" }, "paralog": false, "identity": 0.9791666666666666, "score": 463.0, "evalue": 1.02e-168, "description": "Internal stop codon at 526399, 526465. Frameshift due to insertion around 526880.", "psc": { "uniref90_id": "UniRef90_A0A6A8PYM9", "product": "site-specific DNA-methyltransferase (adenine-specific)", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A6A8PYM9", "kegg_orthology_id": "K06223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8PYM9", "EC:2.1.1.72", "UniRef:UniRef50_A0A6A8PYM9", "KEGG:K06223" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PYM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PYM9" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" } }, "id": "AHCKFKLKGB_896", "locus": "GCA013367975_00895" }, { "type": "cds", "sequence": "contig_1", "start": 526860, "stop": 527240, "strand": "-", "frame": 2, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BBF45A" ], "nt": "ATGGGTTCTGGTTCGACTTGCGTTGCGGCCTTAAAATTGCAAAGGAATTTTATTGGATGTGAGATAGATAAAAAATATTTTTGTGCTGCAAAGCAAAGAATAGAAAGGTATGAGCTAGAAATGAATAAAATGAATAGATCACCATTGTATTATTTGGGCGATAAATATAAATTATTACCACAATTAAAAAAATTATTTCCAAATAATATTAGAAATTTTTATGATGCATTTGCCGGAGGAGGAACGGTTATTGCAAATGTTATAGCTCAAAAATATTTTTACAATGATAAGCAAAAAGAAGTGTGCAATATTGTAAAACTTCTCTCAAATACTAACCCTATAAAAATTTTAAATGATATATATATATATATATATATATAA", "aa": "MGSGSTCVAALKLQRNFIGCEIDKKYFCAAKQRIERYELEMNKMNRSPLYYLGDKYKLLPQLKKLFPNNIRNFYDAFAGGGTVIANVIAQKYFYNDKQKEVCNIVKLLSNTNPIKILNDIYIYIYI", "aa_hexdigest": "056c446d02ba95eae2c1974a810e8ed7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0035BBF45A", "query_cov": 0.9920634920634921, "subject_cov": 0.8620689655172413, "identity": 0.568, "score": 137.0, "evalue": 3.07e-39, "valid": false, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0035BBF45A" }, "pscc": { "uniref50_id": "UniRef50_UPI0035BBF45A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BBF45A" ], "product": "DNA methyltransferase" }, "genes": [], "id": "AHCKFKLKGB_897", "locus": "GCA013367975_00896" }, { "type": "cds", "sequence": "contig_1", "start": 528051, "stop": 528179, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTTCAGGCTTAGCTATTGATAGAGTTGTTGCTGCTATTTTAGAAAATTATCAAAACCCAGATGGTTCAATCACAATTCCAAAAGTTTTAGTTCCATACATGGGCAAAGAAGTTATTAAATAA", "aa": "MNGSGLAIDRVVAAILENYQNPDGSITIPKVLVPYMGKEVIK", "aa_hexdigest": "daddee63f10a648e665742e1012dd38b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_M4S1T5", "query_cov": 0.8809523809523809, "subject_cov": 0.8043478260869565, "identity": 0.649, "score": 54.7, "evalue": 3.78e-09, "valid": false, "product": "Seryl-tRNA(Ser/Sec) synthetase", "uniref50_id": "UniRef50_M4S1T5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4515.254900000001, "isoelectric_point": 5.97095890045166 }, "id": "AHCKFKLKGB_898", "locus": "GCA013367975_00897" }, { "type": "cds", "sequence": "contig_1", "start": 528300, "stop": 528416, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAAAGATGCCAAAAGCATTCTTGAATTGCTTGAAATCCCATACCGTGAAATGATGCTTTCAACTGGCGATATTGGATTTAGCTCAGCAAAAACTATTGACCTAGAACTATGA", "aa": "MVKDAKSILELLEIPYREMMLSTGDIGFSSAKTIDLEL", "aa_hexdigest": "bbfbe33197e43c71e172b25015741629", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4258.968699999999, "isoelectric_point": 4.440682792663575 }, "id": "AHCKFKLKGB_899", "locus": "GCA013367975_00898" }, { "type": "cds", "sequence": "contig_1", "start": 528423, "stop": 528578, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGTCTAAGCCAAAGAAATTTGTTGCATATACAAAATGTTTCCGTTCAGAAGCAGGAAGTAGTGGAAAAGATACAAAAGGTTTAATTAGACAACACGAATTCCATAAAGTAGAACTTGTAAAAATAACCAGTGAAAAAGATGCAATGAAAGAATGA", "aa": "MSKPKKFVAYTKCFRSEAGSSGKDTKGLIRQHEFHKVELVKITSEKDAMKE", "aa_hexdigest": "1bf5c3511f53ff80841e97b1b2e2591e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 46, "aa_cov": 0.9019607843137255, "hmm_cov": 0.9130434782608695, "evalue": 3.7802384305444934e-11, "score": 38.68495178222656, "start": 4, "stop": 49 } ], "seq_stats": { "molecular_weight": 5830.716199999999, "isoelectric_point": 9.61141414642334 }, "id": "AHCKFKLKGB_900", "locus": "GCA013367975_00899" }, { "type": "cds", "sequence": "contig_1", "start": 528636, "stop": 529316, "strand": "-", "frame": 2, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "COG:COG0172", "COG:J", "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_Q98RH5", "UniRef:UniRef90_D1J8K7" ], "nt": "ATGTTAGATTTAAAATATATTATTAGTAACAAAAATGAAGTTATTAAAAAACTATCAACACGTAATTTCAATATTGCTATCATAGAAGAAATTTTTGAAGACGCTCAAAAAAGAAACGAATTAATTCATAATTTGGAAACTTTGCAAGCCAAAAGAAATAAATTAAGTGAACAAATTGGAATTAATAAAAGAAACAAGCAAGATTGCTCGGAATTATTACAACAAGTTGCTGAAGTTAAAGAACAAATTGAAAAAATTGATGAAGAAGCTGACACATTGGTAAAGAAAGTCAATACATTGCTATACCAAATTCCTAACTTGCCATATGATGAAGTTCCTGTGGGAAGAGATGAAGATGACAACGTAGTTTTAAAAGAACATCCAAATTTAGGAAGAGGGTTAGTAAAAGGCGTATTGCCACATTATGAAATAGCAACAAAATTAGATATTGTTGATTTTGTTAGATCAGTGAAGTTAGCAAGCACAAGATTTGTTTTATACAAAAATGATGGTGCTAAATTAGTAAGATCCTTAGCCTCTTTTATGCTTGATACGCACATTGCAAATGGATATAAAGAATTAATGCCTGCCCATTTAGTTAATTCAAATATGCTTTATGGAACGGGACAATTGCCTAAATTTGCAGAAGATTTATTTAAAATGCAAGGCAATGACCTTTGA", "aa": "MLDLKYIISNKNEVIKKLSTRNFNIAIIEEIFEDAQKRNELIHNLETLQAKRNKLSEQIGINKRNKQDCSELLQQVAEVKEQIEKIDEEADTLVKKVNTLLYQIPNLPYDEVPVGRDEDDNVVLKEHPNLGRGLVKGVLPHYEIATKLDIVDFVRSVKLASTRFVLYKNDGAKLVRSLASFMLDTHIANGYKELMPAHLVNSNMLYGTGQLPKFAEDLFKMQGNDL", "aa_hexdigest": "799214d340c25b0e645f110351528f7b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "serS" ], "truncated": "5-prime", "pseudogene": { "start": 528333, "stop": 529316, "observations": { "mutation": [ 528425, 528638 ], "stop": [ 528425, 528638 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K7", "query_cov": 1.0, "subject_cov": 0.5368171021377672, "identity": 1.0, "score": 376.0, "evalue": 8.29e-128, "gene_start": 1, "gene_end": 226, "reference_start": 1, "reference_end": 226, "reference_sequence": "MLDLKYIISNKNEVIKKLSTRNFNIAIIEEIFEDAQKRNELIHNLETLQAKRNKLSEQIGINKRNKQDCSELLQQVAEVKEQIEKIDEEADTLVKKVNTLLYQIPNLPYDEVPVGRDEDDNVVLKEHPNLGRGLVKGVLPHYEIATKLDIVDFVRSVKLASTRFVLYKNDGAKLVRSLASFMLDTHIANGYKELMPAHLVNSNMLYGTGQLPKFAEDLFKMQGNDLWLIPTAEVPVTNYYYDEILDLSKPKKFVAYTKCFRSEAGSSGKDTKGLIRQHEFHKVELVKITSEKDAMKEWESMVNDAKSILELLEIPYREMMLSTGDIGFSSAKTIDLELWIPSEQRYRETSSISICRDFQGRRAKIRYRDETGHTKYAYTMNGSGLAIDRVVAAILENYQNPDGSITIPKVLVPYMGKEVIK" }, "paralog": false, "identity": 0.9877675840978594, "score": 571.0, "evalue": 4.96e-207, "description": "Internal stop codon at 528425, 528638. Nonsense mutation around 528425, 528638.", "psc": { "uniref90_id": "UniRef90_D1J8K7", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_Q98RH5", "cog_id": "COG0172", "cog_category": "J", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K7", "EC:6.1.1.11", "UniRef:UniRef50_Q98RH5", "COG:COG0172", "COG:J", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RH5" ], "product": "Serine--tRNA ligase" } }, "id": "AHCKFKLKGB_901", "locus": "GCA013367975_00900" }, { "type": "cds", "sequence": "contig_1", "start": 529569, "stop": 529943, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTATCTTCAAACGATGGTTTGAATCCTGTTAAAGTTAATAAATACAATAGTATTTCAGTAACTTTAAAAAACAATTTATTTTCTGAAAAAAACATTACCTTAAACAGCCACATTTATGAAATATCAGTCTCTAAAAATAGTTTTTCAAAATATCCAAATAGTTATATTGCAACAGACGTTATTGAAATAAACGAAGATGAAGAATATAAACTAAGCTATGTTATTAAATTTTTAAAAAGCAGTCATTTCCCTGGTATAGGTGAAGAAAAAGCAAAACTAATTGTAAAAGATTTAGGGATCGATGCTTTAAGAAAAATGGTTGAGGATAATTCAATTGATTATTCAAAATATTCAATATCAGAATTGACATGA", "aa": "MVSSNDGLNPVKVNKYNSISVTLKNNLFSEKNITLNSHIYEISVSKNSFSKYPNSYIATDVIEINEDEEYKLSYVIKFLKSSHFPGIGEEKAKLIVKDLGIDALRKMVEDNSIDYSKYSISELT", "aa_hexdigest": "c5775b92834c8c61b546ba38425c57d3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14064.742899999987, "isoelectric_point": 5.683467292785645 }, "id": "AHCKFKLKGB_902", "locus": "GCA013367975_00901" }, { "type": "cds", "sequence": "contig_1", "start": 529995, "stop": 530306, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTTTTAACAAATAATTTAACGCCTTCTTTGTATGAAATAGTTGCTAAGAAATTTGAACATTTTAAAGATTTTATAGATGCTTACAAAGATAACTTTTACTTATTTTATTTAGACAATGAACGTATCTCACTTGAAGATATGGATAAACTGCATAATATCTTTAACAAATTTGAACATCCTTTTAAGCCTGCAACTCACTTGTACAAATCATTTTTAAAATATTTATACAATTCCGGAAACACTAGAATTTTAAAGGATTGTTTATATGATCAACTAATAAACTTTGCAAGTCAAGACACTGAATGA", "aa": "MFFLTNNLTPSLYEIVAKKFEHFKDFIDAYKDNFYLFYLDNERISLEDMDKLHNIFNKFEHPFKPATHLYKSFLKYLYNSGNTRILKDCLYDQLINFASQDTE", "aa_hexdigest": "9c79dd19ffb3c65c5d1ec55f7e878abf", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12514.065299999982, "isoelectric_point": 5.754629707336425 }, "id": "AHCKFKLKGB_903", "locus": "GCA013367975_00902" }, { "type": "cds", "sequence": "contig_1", "start": 530346, "stop": 530741, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13245.12", "PFAM:PF13604.12" ], "nt": "TTGGTTCAAAATAAATATTTAATTGAGATTGAATACGAAGATGGTACATACCTTAGCGCCAAAGATGTATTTGATATGGAAAAATATATTGTCAAAAGATTGAAACATATTGAATCTGCTTCAAACCCTAAATTCATTAATTTCGTGCCAAGTGAAATGTTCCACCCTTTGCAACTTCAGGCAATTGAAACAGCATTAACTAGCAAGTTAACATTGATCACAGGAAACCCCGGTACCGGAAAAACGTTAATAACCAATGAAATAATCAAACAACTACTTTCAGTTTATGATGAAGATGATTTGGCAATAGTTACACCAACCGGTAGAGCAACTATCAACATAAACAATATTCAAGAAAAAACAAATGCAGTTACTATACATAGTTTTTTAGAATGA", "aa": "MVQNKYLIEIEYEDGTYLSAKDVFDMEKYIVKRLKHIESASNPKFINFVPSEMFHPLQLQAIETALTSKLTLITGNPGTGKTLITNEIIKQLLSVYDEDDLAIVTPTGRATININNIQEKTNAVTIHSFLE", "aa_hexdigest": "cba1e58a1fa46968f3fe89d1cb33bd34", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13245.12", "name": "AAA_19", "length": 73, "aa_cov": 0.5572519083969466, "hmm_cov": 0.9863013698630136, "evalue": 5.0473880997975415e-18, "score": 60.89728927612305, "start": 58, "stop": 130 }, { "id": "PF13604.12", "name": "AAA_30", "length": 77, "aa_cov": 0.5725190839694656, "hmm_cov": 0.961038961038961, "evalue": 2.9787573407034395e-08, "score": 28.938627243041992, "start": 56, "stop": 130 } ], "seq_stats": { "molecular_weight": 14801.797799999977, "isoelectric_point": 5.177486610412596 }, "id": "AHCKFKLKGB_904", "locus": "GCA013367975_00903" }, { "type": "cds", "sequence": "contig_1", "start": 530763, "stop": 531728, "strand": "+", "frame": 3, "gene": "recD", "product": "Exodeoxyribonuclease V alpha chain", "db_xrefs": [ "COG:COG0507", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J8K8", "UniRef:UniRef90_D1J8K8" ], "nt": "ATGATTAATGAAAAAAATGCAAAACCGATTGAATGTTTAATAATTGATGAATTTTCAATGGTAAGTGTTGATTTGTTTTATTCACTACTTAAAGGAATAAAAAAGAATTCGTTGAAAAGAATTATATTAGTTGGCGACAAAGATCAATTGCCTGCCATTGGCGCTGGTTATTTAATTAGGGATTTTATAGAAAGCAATATTTTTCAAACAATTGTTTTAAGTAAAATTTTTAGACAAGCAGAAAATTTTGACATTATTCAAGACGCTCTAGATATTAATAAAGGCAAACTACCCGAATTTAAAGGAAATAATTCTCAGTTTATCGAAACGAAACAGAGTGATCTAAAGTCAATGTTGCTCTTTAAATTAAATGACTTATTACAGCAAGGATATAGCAAGCGTGATATTGCAATTTTAAGTCCAATATATAATTACGAAACGGGAATTGACCAAATAAATGAAACATTAAATAATTTCTTTAGAAAATTAGATGGCAATGAACAAATAAAATATAGAGACCATATTTTGGCCATTGATGATAAGGTAATAAATTTAGTTAATGATCCAAAAACAAAAGTGTTTAATGGAGAAATCGGTTATATATCTCACTTTGGCTTTGAAAACAAAAAAAATTCTTTTGAAAAGGTTTTAGCAAGTATTTCTGTTGATTTTGAAAACGATTCAAAAACTGTTTCTTATTCTAGAAAAGATTTTTTTGCTAATACATACCCTGCATATTGCACAAGCGTTCATAAATATCAAGGTAGTGAATGTAAATGCGTTATTGTAGTATTATTTTCAGAGGCTAAAAAATTATTATCAAAAAAACTTATTTATACCGCTATTACCAGAGCAAAAAAATATTGTGTTGTTATTGGAGAAAAAGAAGCTTTGATAACCGGTATAAATAATGATGATGATTCAAATAGAATAACCAATATTAAATATTTATGGAGGAACGAATAA", "aa": "MINEKNAKPIECLIIDEFSMVSVDLFYSLLKGIKKNSLKRIILVGDKDQLPAIGAGYLIRDFIESNIFQTIVLSKIFRQAENFDIIQDALDINKGKLPEFKGNNSQFIETKQSDLKSMLLFKLNDLLQQGYSKRDIAILSPIYNYETGIDQINETLNNFFRKLDGNEQIKYRDHILAIDDKVINLVNDPKTKVFNGEIGYISHFGFENKKNSFEKVLASISVDFENDSKTVSYSRKDFFANTYPAYCTSVHKYQGSECKCVIVVLFSEAKKLLSKKLIYTAITRAKKYCVVIGEKEALITGINNDDDSNRITNIKYLWRNE", "aa_hexdigest": "eb9b1439232604d5afe735a4d0308b36", "start_type": "ATG", "rbs_motif": null, "genes": [ "recD" ], "truncated": "5-prime", "pseudogene": { "start": 530463, "stop": 531728, "observations": { "mutation": [ 530739 ], "stop": [ 530739 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K8", "query_cov": 1.0, "subject_cov": 0.42971887550200805, "identity": 1.0, "score": 633.0, "evalue": 1.19e-222, "gene_start": 1, "gene_end": 321, "reference_start": 427, "reference_end": 747, "reference_sequence": "MLQNSNIQGKFNKIMWTSTDGQVVIVSFLVSSNDGLNPVKVNKYNSISVTLKNNLFSEKNVTLNSHIYEISVSKNSFSKYPNSYIATDVIEINEDEEYKLNYVIKFLKSSHFPGIGEEKAKLIVKDLGIDALRKMVEDNSIDYSKYSISELTWNYAVEFIKNNPTLIEDQMFFLTNNLTPSLYEIVAKKFEHFKDFIDAYKDNFYLFYLDNERISLEDMDKLHNIFNKFEHPFKPATHLYKSFLKYLYNSGNTRILKDYLYDQLINFASQDTEWPRDVETFNNSVELLVQNKYLIEIEYEDGTYLSAKDVFDMEKYIVKRLKHIESSSNPKFINFMPSEMFHPLQLQAIETALTSKLTLITGNPGTGKTLITNEIIKQLLSVYDEDDLAIVTPTGRATININNIQEKTNAVTIHSFLEWDVDNDKFMINEKNAKPIECLIIDEFSMVSVDLFYSLLKGIKKNSLKRIILVGDKDQLPAIGAGYLIRDFIESNIFQTIVLSKIFRQAENFDIIQDALDINKGKLPEFKGNNSQFIETKQSDLKSMLLFKLNDLLQQGYSKRDIAILSPIYNYETGIDQINETLNNFFRKLDGNEQIKYRDHILAIDDKVINLVNDPKTKVFNGEIGYISHFGFENKKNSFEKVLASISVDFENDSKTVSYSRKDFFANTYPAYCTSVHKYQGSECKCVIVVLFSEAKKLLSKKLIYTAITRAKKYCVVIGEKEALITGINNDDDSNRITNIKYLWRNE" }, "paralog": false, "identity": 0.9928741092636579, "score": 826.0, "evalue": 3.93e-301, "description": "Internal stop codon at 530739. Nonsense mutation around 530739.", "psc": { "uniref90_id": "UniRef90_D1J8K8", "gene": "RecD", "product": "Exodeoxyribonuclease V alpha chain", "uniref50_id": "UniRef50_D1J8K8", "cog_id": "COG0507", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K8", "UniRef:UniRef50_D1J8K8", "COG:COG0507", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8K8" ], "product": "Exodeoxyribonuclease V alpha chain" } }, "id": "AHCKFKLKGB_905", "locus": "GCA013367975_00904" }, { "type": "cds", "sequence": "contig_1", "start": 531728, "stop": 532288, "strand": "+", "frame": 2, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "RefSeq:WP_012855695.1", "SO:0001217", "UniParc:UPI0001BC2435", "UniRef:UniRef100_D1J8K9", "UniRef:UniRef50_Q98PE2", "UniRef:UniRef90_D1J8K9" ], "nt": "ATGAAATTAATTGTTGGACTTGGAAATCCTGGTGTTGAATATGAAAAAACACGCCATAACACAGGGTTTATGGTAATTGACAAACTAGCAGAAAAATTGCATACTCCCTTAACTGAAAAAAAGTTTAATGGAATTTATTACATTGACAAAGAAGTTATTTTAGCCAAACCGTTAACCTTTATGAATAATAGCGGTGATTTTGTTAGAGCAATAAAGGATTATTACAATATTGTAGATGATGACATTATCATTGTATATGATGATTTAGATTTACCAGTTGGAAAAGCTGTTATTAGACAAAAAGGTTCGTCTGGTGGACATAATGGAATGAAAGATATAATTGCTAAATTAAAAACTGATGAAATAAAAAGATTAAAAATAGGAATTGGACGTGGTCAGAATGTAATTAATTATGTTCTTGGCAAATTTAGCTATCAAGATTTTTGCACAATTGAAAAAATAATTGATATTGCTTCTGATGCATTAATTTCTTTTATTAGTAATGATATTAGATTTGTTATGAATAAATTTAGTGGTGAACTATATCGTGAAAACAAATAA", "aa": "MKLIVGLGNPGVEYEKTRHNTGFMVIDKLAEKLHTPLTEKKFNGIYYIDKEVILAKPLTFMNNSGDFVRAIKDYYNIVDDDIIIVYDDLDLPVGKAVIRQKGSSGGHNGMKDIIAKLKTDEIKRLKIGIGRGQNVINYVLGKFSYQDFCTIEKIIDIASDALISFISNDIRFVMNKFSGELYRENK", "aa_hexdigest": "ffa6914d0b626ec4bf14b6ed375ee7a8", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001BC2435", "ncbi_nrp_id": "WP_012855695.1", "uniref100_id": "UniRef100_D1J8K9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2435", "RefSeq:WP_012855695.1", "UniRef:UniRef100_D1J8K9" ] }, "ips": { "uniref100_id": "UniRef100_D1J8K9", "uniref90_id": "UniRef90_D1J8K9" }, "psc": { "uniref90_id": "UniRef90_D1J8K9", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_Q98PE2", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PE2" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "AHCKFKLKGB_906", "locus": "GCA013367975_00905" }, { "type": "cds", "sequence": "contig_1", "start": 532275, "stop": 532709, "strand": "+", "frame": 3, "gene": "ttcA", "product": "tRNA(Ile)-lysidine synthase", "db_xrefs": [ "COG:COG0037", "COG:J", "EC:6.3.4.19", "KEGG:K04075", "SO:0001217", "UniRef:UniRef50_D1J8L0", "UniRef:UniRef90_D1J8L0" ], "nt": "GTGAAAACAAATAATTTAGTTGAATTACAACGTGACTTATTAAATGAATTTAATAAATACAATATTAATACCAATAAAAAATTTTTAATCGGAGTAAGTGGTGGCCCTGATTCAATGTGGCTACTTAATTTGCTTAAATCATTGAATATTGTTGTTGCACATGTAAACTACAACAAAAGATATGATAGCAATTATGATGAATCATTAGTTTCACATTTTTGTTTAGATAACAATATCCCTTTAGAAATACTCTCATTACATAACAAATATTCTAGTGGTAACTTCCAAGCTATTGCTAGAAAAGAAAGATATGATTTTTACTTTGAAATTTCTAAAAAATATCAATGTGACTATATCCTGCTTGCTCACCAAAAAGATGATTTTTTAGAATCATCTATTATGCAAGAAAATTCAAACAGAAAACCTTTATTTTGA", "aa": "MKTNNLVELQRDLLNEFNKYNINTNKKFLIGVSGGPDSMWLLNLLKSLNIVVAHVNYNKRYDSNYDESLVSHFCLDNNIPLEILSLHNKYSSGNFQAIARKERYDFYFEISKKYQCDYILLAHQKDDFLESSIMQENSNRKPLF", "aa_hexdigest": "fcea16db5d4604b92edcbd83df439ccf", "start_type": "GTG", "rbs_motif": null, "genes": [ "ttcA" ], "truncated": "3-prime", "pseudogene": { "start": 532275, "stop": 533012, "observations": { "mutation": [ 532707, 532770, 532986 ], "stop": [ 532707, 532770, 532986 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L0", "query_cov": 1.0, "subject_cov": 0.4630225080385852, "identity": 0.972, "score": 281.0, "evalue": 1.3e-93, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MKTNNLVELQRDLLNEFNKCNIDTNKKFLIGVSGGPDSMWLLNLLKSLNIVVAHVNYNKRYDSNYDESLVSQFCADNNIPLEILSLHNKYSSGNFQAIARKERYDFYFEISKKYQCDYILLAHQKDDFLESSIMQENSNRKPLFWGIKQLSVLNNNIIFRPMLNLWTKQEIIDFCNEYSILFATDYTNAETHYTRNKIRSNLKKLDPEQKQNKYQFFVNKNIENEHIEKQVLESFEDWKNNQFNVDKLDKNFVYIDQLLFYTLNHFYNNLKLSLNKIHGIKDFLFSQNRTKNFKLNDEQYIHKIKNLLIFK" }, "paralog": false, "identity": 0.9551020408163265, "score": 475.0, "evalue": 2.99e-172, "description": "Internal stop codon at 532707, 532770, 532986. Nonsense mutation around 532707, 532770, 532986.", "psc": { "uniref90_id": "UniRef90_D1J8L0", "gene": "TtcA", "product": "tRNA(Ile)-lysidine synthase", "ec_ids": [ "6.3.4.19" ], "uniref50_id": "UniRef50_D1J8L0", "cog_id": "COG0037", "cog_category": "J", "kegg_orthology_id": "K04075", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L0", "EC:6.3.4.19", "UniRef:UniRef50_D1J8L0", "COG:COG0037", "COG:J", "KEGG:K04075" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8L0" ], "product": "tRNA(Ile)-lysidine synthase" } }, "id": "AHCKFKLKGB_907", "locus": "GCA013367975_00906" }, { "type": "cds", "sequence": "contig_1", "start": 533258, "stop": 533467, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAATCCAAAAGAAAGAAAACCTAGGAAAATTGGTTTGTTTGGAATAATTGGAATTTTATTTGCAATTATTGCAGTGATAACATTGGGATATATTATCAGTGATGTTGTTAAAGAAAGAATGTCTAACTTTCAAACCTTAAATTTTCTAGAAAGAGTTATTAAAGGAACTGCTAACCCAAATGATGGAAACTATATTTCTTCATGA", "aa": "MNNPKERKPRKIGLFGIIGILFAIIAVITLGYIISDVVKERMSNFQTLNFLERVIKGTANPNDGNYISS", "aa_hexdigest": "189647d5b2ec5f051b6e0b51fba013d5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7710.971, "isoelectric_point": 9.867998313903808 }, "id": "AHCKFKLKGB_908", "locus": "GCA013367975_00907" }, { "type": "cds", "sequence": "contig_1", "start": 533666, "stop": 534967, "strand": "+", "frame": 2, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A485JC26" ], "nt": "TTGAATTCATGTGCAACATTTAATGGCCAAGTTTTAGGAAATATTGTTGCAAGTTCAATGCCAAAACCAAGCATTGCAAGCAAATATATAATTCCTGCTTTCTCTGCTTTTGTCGGCTTAGCATTTATTATTATCATTATGGCAATGTATTTTGGTAAGGGAAGAGGAATGTTAACTGGCGGTATTGATACAAATAAATCATTAGCGCAAAGAATATATTCAAATAAAAAATTCTCTGACATTGCCGGAAATGAAGAAGTAAAAGAAGAAGTAAAAGAATTAGTTGATTATCTTAAAAATCCTAAAAAATATGAAGCAGCCGGTGCTAGAATTCCAAAAGGAATATTATTAGGAGGCCCTCCAGGAACTGGTAAAACTTTAATTGCCAAAGCAACAGCGGGAGAAGCAAATGTTCCATTCTTCTTCATCTCGGCATCAAATTTTGTTGAAATGTTTGTTGGATTAGGTGCAAAAAGAGTTCGTGATATGTTTGCTGAAGCTCGCTCTCATGCTCCTGCAATAATTTTCATTGACGAATTAGATGCTGTTGGTAGATCTCGTGGAGCTGGAATTGGTGGAGGTAATGATGAAAGAGAACAAACTCTTAACCAATTACTAGTAGAAATGGATGGTATCAAAGAAAATAGTGGTATTTTAATAATGGCAGCTACAAATAGAACCGACGTTCTAGACCCTGCTTTATTAAGACCAGGACGTTTTGATAGAACTATTACCGTTGGCCTTCCTGATATAAAAGAAAGAGAAGCAATTTTAAAATTACATGCTAATGGAAAAAGAATTGCAAGCAATATTAATTTTGCTATGGTAGCAAGAAGAACACCTGGTTTCTCTGGAGCACAGCTTGAAAATGTAATTAACGAAGCCTCTTTATTAAGCGTTAGAGAAAATACCGGAGTTATAACTATTGAACAAATAGATGAAGCTATTGACAGAGTTATTGCCGGCCCTGCTAAAAAATCAAGAACAATTTCTGAAAAAGAAAACATTGCAGTTGCCTACCATGAAGCGGGGCATGCAGTTGTTGGAATAAAAATTAAAGGCGGAAATAAAGTTCAAAAAATAACTATTATTCCAAGAGGAAAAGCTGGCGGATATAATTTAATGATGCCAGATGAAGAAAAATACAATCGCTCGAAATCAGAATTACTAGCTATAATAACTTCGTTCATGGGAGGACGTGTAGCTGAAGCAATTATTTATGGAGCAGATAATGTTTCAACTGGAGCAAGTGACGATATAGCTAAAGCAACAAATATTGCTAGAAAAATGGTTACTGAATGA", "aa": "MNSCATFNGQVLGNIVASSMPKPSIASKYIIPAFSAFVGLAFIIIIMAMYFGKGRGMLTGGIDTNKSLAQRIYSNKKFSDIAGNEEVKEEVKELVDYLKNPKKYEAAGARIPKGILLGGPPGTGKTLIAKATAGEANVPFFFISASNFVEMFVGLGAKRVRDMFAEARSHAPAIIFIDELDAVGRSRGAGIGGGNDEREQTLNQLLVEMDGIKENSGILIMAATNRTDVLDPALLRPGRFDRTITVGLPDIKEREAILKLHANGKRIASNINFAMVARRTPGFSGAQLENVINEASLLSVRENTGVITIEQIDEAIDRVIAGPAKKSRTISEKENIAVAYHEAGHAVVGIKIKGGNKVQKITIIPRGKAGGYNLMMPDEEKYNRSKSELLAIITSFMGGRVAEAIIYGADNVSTGASDDIAKATNIARKMVTE", "aa_hexdigest": "fcec13ee6b4abdd3b5e43362c31ab41a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A485JC26", "query_cov": 0.9468822170900693, "subject_cov": 0.8102766798418972, "identity": 0.512, "score": 403.0, "evalue": 5.68e-134, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "ec_ids": [ "3.4.24.-" ], "uniref50_id": "UniRef50_A0A485JC26", "go_ids": [ "GO:0004176", "GO:0004222", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_A0A485JC26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A485JC26" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "AHCKFKLKGB_909", "locus": "GCA013367975_00908" }, { "type": "cds", "sequence": "contig_1", "start": 535037, "stop": 535477, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01434.24" ], "nt": "ATGAAAAATTCTTCATTTTCAAGTAAAGTAGGCCAAGAAATTGATGAACAAATAAGAAAAATTATTTTAACCGCTGAAAAGAAAGCACATGAAATAATTACAGAAAATCGTCAATTGTTAGAATTAATTAAAGACGCATTAATTATTAAAGAAACTATTGTTGCTGAAGAAATTGAATATATAGAAAAACATATGGAATTGCCTCCTGAAATAACAAATACAAAAAAAGAACTTAAAAATGAATATTCAGAAGAAGATTTCAATGAATTATTTAATGAAGTTGCTGGACAAAAACAAATTCAAGAAGATAAATACAAAGCTACTTTAAACTCTGAATTAAAGAAACATAATATTGAAGATAAAAGTTCAAGCAACCATTCAGAAGATTCTAATGATACAAAAAATTCAGATGAAGACAGCAACAAAGAAGACAATCAATAA", "aa": "MKNSSFSSKVGQEIDEQIRKIILTAEKKAHEIITENRQLLELIKDALIIKETIVAEEIEYIEKHMELPPEITNTKKELKNEYSEEDFNELFNEVAGQKQIQEDKYKATLNSELKKHNIEDKSSSNHSEDSNDTKNSDEDSNKEDNQ", "aa_hexdigest": "1f5b3c030b557546c811f293570fc286", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01434.24", "name": "Peptidase_M41", "length": 59, "aa_cov": 0.4041095890410959, "hmm_cov": 1.0, "evalue": 6.932749131202987e-11, "score": 37.650604248046875, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 16967.47839999999, "isoelectric_point": 4.740451622009277 }, "id": "AHCKFKLKGB_910", "locus": "GCA013367975_00909" }, { "type": "cds", "sequence": "contig_1", "start": 535503, "stop": 535856, "strand": "-", "frame": 2, "gene": "ygaC", "product": "DUF402 domain-containing protein", "db_xrefs": [ "COG:COG3557", "COG:S", "KEGG:K07586", "SO:0001217", "UniRef:UniRef50_A0AA43TZF8", "UniRef:UniRef90_D1J8L2" ], "nt": "TTGTTTTTCTTTGCTAGAAACAAATTTTATAATGCTTCTATTACATTAAATAAATCTAAAGAAAACTATATTTATATTAATTTAGCAAGCCCTTTTTATATTGAAAATGAAGTAATTAAATACATTGATTTTGATATCGATATTAAAAGTTATGATGATAAAGATTTTAGCGTTATAGATTGGCAAGATTTTAAAAATTCAATTAGTCAATACAATTATTCATTGAAATTAATTTATAAATTGTATGAAGAATTAGATTTTTTATATGAACAAAAGCAAAAAGGAACAGGAGTTTTTTCTAAGAAACTTGTTAACGGCCTTGAAAAGATGTTGCGAGAAAGCGGAGATATATAA", "aa": "MFFFARNKFYNASITLNKSKENYIYINLASPFYIENEVIKYIDFDIDIKSYDDKDFSVIDWQDFKNSISQYNYSLKLIYKLYEELDFLYEQKQKGTGVFSKKLVNGLEKMLRESGDI", "aa_hexdigest": "3b42402dbf2f9f440d25f246ec1fd3a3", "start_type": "TTG", "rbs_motif": null, "genes": [ "ygaC" ], "truncated": "3-prime", "pseudogene": { "start": 535503, "stop": 536117, "observations": { "mutation": [ 535877 ], "stop": [ 535877 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L2", "query_cov": 1.0, "subject_cov": 0.5735294117647058, "identity": 0.991, "score": 228.0, "evalue": 2.52e-74, "gene_start": 1, "gene_end": 117, "reference_start": 88, "reference_end": 204, "reference_sequence": "MEEESIKSFDDLLKTIDTPNSKNIKKHKKFFKMGQLTTIQVFKYDGTLYRQYEGAKIVANLTDFVVVLLVKTKVSEKSINWVVSEPILFFFARNKFYNASITLNKSKENYIYINLASPFYIENEVIKYIDFDIDIKSYDDKDFSVIDWQDFKNSISQYNYSLKLIYKLYEELDFLYEQKQKGTGVFSKKLVNGLEKMLRESGDI" }, "paralog": false, "identity": 0.9901960784313726, "score": 401.0, "evalue": 3.75e-145, "description": "Internal stop codon at 535877. Nonsense mutation around 535877.", "psc": { "uniref90_id": "UniRef90_D1J8L2", "gene": "YgaC", "product": "DUF402 domain-containing protein", "uniref50_id": "UniRef50_A0AA43TZF8", "cog_id": "COG3557", "cog_category": "S", "kegg_orthology_id": "K07586", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L2", "UniRef:UniRef50_A0AA43TZF8", "COG:COG3557", "COG:S", "KEGG:K07586" ] }, "pscc": { "uniref50_id": "UniRef50_A0AA43TZF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA43TZF8" ], "product": "DUF402 domain-containing protein" } }, "id": "AHCKFKLKGB_911", "locus": "GCA013367975_00910" }, { "type": "cds", "sequence": "contig_1", "start": 535875, "stop": 536117, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGAAAGTATAAAGAGTTTTGATGATTTATTAAAAACAATTGATACACCAAACTCAAAAAATATAAAAAAACATAAAAAGTTTTTTAAGATGGGACAACTAACAACCATTCAAGTTTTTAAATATGATGGAACTTTATACAGACAATATGAAGGGGCAAAAATAGTTGCAAATTTAACTGATTTTGTAGTTGTTTTGTTAATGAAAACAAAAGTTTCTGAAAAATCTATCAATTGA", "aa": "MEEESIKSFDDLLKTIDTPNSKNIKKHKKFFKMGQLTTIQVFKYDGTLYRQYEGAKIVANLTDFVVVLLMKTKVSEKSIN", "aa_hexdigest": "94f70607d96c17d2aa44eef453fc226f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9277.7353, "isoelectric_point": 9.363597679138184 }, "id": "AHCKFKLKGB_912", "locus": "GCA013367975_00911" }, { "type": "cds", "sequence": "contig_1", "start": 536372, "stop": 536638, "strand": "+", "frame": 2, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_Q9F411", "UniRef:UniRef90_Q9F411" ], "nt": "ATGGCAAGCCCAATGACATTTAAGGATTTTCCTATTGATAATAAAAAGAATACTATATTAGAGATTGGTATGGGGAGAGGAACCATGCTTACAAAATTAGCCCTAAAGCATCCTAACATAGAATATATTGGACTTGAAAAATACAGCACGCCAGCTTATTCTGCTTTAAAAAAGGCAATTGACCTCAAACTTGAAAATTTTCACATAATTATAGGAGATGCAATAAATTTATCAACTTATTTTAAAAACAAAATTAAAACTATTTGA", "aa": "MASPMTFKDFPIDNKKNTILEIGMGRGTMLTKLALKHPNIEYIGLEKYSTPAYSALKKAIDLKLENFHIIIGDAINLSTYFKNKIKTI", "aa_hexdigest": "aaae6baba504ec1605de098b08818a11", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 536333, "stop": 536941, "observations": { "mutation": [ 536636, 536657 ], "stop": [ 536636, 536657 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9F411", "query_cov": 1.0, "subject_cov": 0.43564356435643564, "identity": 0.966, "score": 172.0, "evalue": 8.01e-53, "gene_start": 1, "gene_end": 88, "reference_start": 14, "reference_end": 101, "reference_sequence": "MRLRFNKNAETSLMASPMTFKDFPIDNKKNTILEIGMGRGTMLTKLALMHPDIEYIGLEKYSTPAYSALKKAIDLNLENFHIIIGDAINLSTYFKNKIKTIWLTFSDPWPKKRHYKRRLVYRDFLKIYQNVLDKDGVVYFKTDNDMLYQFAIDELKEINAKIIYQTSDLHHCNFKIENVFTDYEEKFNKLNKNINFIAFTFN" }, "paralog": false, "identity": 0.9653465346534653, "score": 395.0, "evalue": 2.2e-143, "description": "Internal stop codon at 536636, 536657. Nonsense mutation around 536636, 536657.", "psc": { "uniref90_id": "UniRef90_Q9F411", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_Q9F411", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9F411", "EC:2.1.1.33", "UniRef:UniRef50_Q9F411", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_Q9F411", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9F411" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "AHCKFKLKGB_913", "locus": "GCA013367975_00912" }, { "type": "cds", "sequence": "contig_1", "start": 536741, "stop": 536941, "strand": "+", "frame": 2, "gene": null, "product": "tRNA (Guanine-N(7)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PL40" ], "nt": "GTGGTTTATTTTAAAACCGATAATGATATGCTTTATCAATTTGCAATAGATGAATTAAAAGAAATTAATGCAAAAATCATTTATCAAACGTCTGATTTACACCATTGCAATTTCGAAATAGAAAATGTTTTTACTGATTATGAAGAAAAATTTAACAAATTAAACAAAAATATAAACTTTATTGCATTTACTTTCAATTAA", "aa": "MVYFKTDNDMLYQFAIDELKEINAKIIYQTSDLHHCNFEIENVFTDYEEKFNKLNKNINFIAFTFN", "aa_hexdigest": "36e27a7026d4feab4277b0b28476646a", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0PL40", "query_cov": 0.9848484848484849, "subject_cov": 0.8441558441558441, "identity": 0.615, "score": 93.6, "evalue": 8.69e-24, "valid": false, "product": "tRNA (Guanine-N(7)-)-methyltransferase", "uniref50_id": "UniRef50_A0A3B0PL40" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PL40", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PL40" ], "product": "tRNA (Guanine-N(7)-)-methyltransferase" }, "genes": [], "id": "AHCKFKLKGB_914", "locus": "GCA013367975_00913" }, { "type": "cds", "sequence": "contig_1", "start": 537016, "stop": 538689, "strand": "+", "frame": 1, "gene": "gyrA", "product": "DNA gyrase subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265", "KEGG:K02469", "SO:0001217", "UniRef:UniRef50_B3PN30", "UniRef:UniRef90_D1J8L4" ], "nt": "ATGCTTTTTGGCGAAGATGATAACAATAAAAAAGAAAATAAAAATTTTGAAGAAGATAACAGCCACGATTTAGATGAATATACAAATTTTGATGATAGAATCAAAAAAATCTTTAGTGACGATGATGAAACAAAAGCAAAAAAAATAGATGAAGAAGACGAAGAAGAAATTCCACAAGACAAAGAAGGCTATAAAGTAGAACCTCAACTTTTAGATAAAGAAATTAATGGTTTAAAACCTGCCAATTTATCAAAAGTAATGAAAACATCATTCATTGAATATGCAATGAGTGTCATAGTTTCTCGTGCTCTCCCTGATGCTCGTGACGGTCTAAAGCCAGTACACCGTAGAATTTTATATGGCATGAGCGAACTTGGAATGTTTTATACAGCGCCACATAAAAAATCGGCAAGAATCGTCGGAGATGTTTTAGGTAAATATCACCCACATGGAGATTCATCAGTATATGAAGCTATGGTAAGAATGGCTCAAGATTTTTCACTTCGCTATCCATTAATTGATGGACATGGTAACTTTGGTTCTGTTGACGGAGATGAAGCTGCCGCAATGCGTTATACAGAAGCTAGAATGAGCAAGATTGCTGGCGCAATGGTTGATGGTATTAAAAAAAATACTGTTGACTTTATGGATAACTATGACGCAACTGAAAAAGAACCAGTTGTTTTGCCATCACGTTTCCCTAATTTATTAGTTTCTGGTTCAAGTGGTATTGCTGTTGGTATGGCAACAAATATTCCTCCTCATAACCTAGGTGAAACTATTGATGCAGTTTGTGCACTTGCAAAAAATCCAGAAATAAATGTTTTTGAATTGATGGAATATATTCAAGCTCCTGATTTTCCAACAGGTGGAATAATATTTAATAAAAAAGGATTAATTGAAGCTTATAGCACAGGTAGAGGATCAATCACCATTCGTTCAAAAGCACACATACAAGAACTTTCAAACGGAAAAAGTAAAATAATTGTTACTGAAATTCCATACGAAGTTAAAAAAACAGAAATCATGGAAAAAATTTCTGAACTTATTAAAGATAAAAAAATTGATGGAATTGCTGATTTTAGAGATGAATCAAATAGAGATGGTATTAGAATTGTAATTGATATCAAAAAATCTTTTGTTCCCGAAATTGTTTTAAATAACTTGTTCAAATTAACAAAATTACAAAGCAATTATTCATTCAATATGATTGCGTTGGTAAATAATGAACCAAAATTGTTGAACCTTAAGGCTTGCTTAGAAGTTTATTTAAAACACCAAATTGAAGTTGTAAGTAGAAGGTTAAAATTTGATCTTGAAAAAGATAGAGCAAGAGCTCATATTTTAGAAGGTTTAAAAATCTGCATTGAAAATATTGATGCCGTAATTAAAATAATTAAAGAATCAAAAACTGACCAAGAAGCACAAAGCAAACTTTCTCAAGCATTTAATCTAAGTGAAATACAAACAAAAGCAATTGTTGATATGAAACTAGGAAGACTAACCGGATTAGCAATTGAAAAAATGAATGAAGAACTAAATGAAGTGCATGAAAGAATAGCTAACTACATTAAGATATTAGGCGACCATAACTTACTAATCAATTTAATAATCGAAGAATTGCAAGAAATTAAAAATGCTTATGGCGATGCTCGTAGAAGCGATATAAATTGA", "aa": "MLFGEDDNNKKENKNFEEDNSHDLDEYTNFDDRIKKIFSDDDETKAKKIDEEDEEEIPQDKEGYKVEPQLLDKEINGLKPANLSKVMKTSFIEYAMSVIVSRALPDARDGLKPVHRRILYGMSELGMFYTAPHKKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSLRYPLIDGHGNFGSVDGDEAAAMRYTEARMSKIAGAMVDGIKKNTVDFMDNYDATEKEPVVLPSRFPNLLVSGSSGIAVGMATNIPPHNLGETIDAVCALAKNPEINVFELMEYIQAPDFPTGGIIFNKKGLIEAYSTGRGSITIRSKAHIQELSNGKSKIIVTEIPYEVKKTEIMEKISELIKDKKIDGIADFRDESNRDGIRIVIDIKKSFVPEIVLNNLFKLTKLQSNYSFNMIALVNNEPKLLNLKACLEVYLKHQIEVVSRRLKFDLEKDRARAHILEGLKICIENIDAVIKIIKESKTDQEAQSKLSQAFNLSEIQTKAIVDMKLGRLTGLAIEKMNEELNEVHERIANYIKILGDHNLLINLIIEELQEIKNAYGDARRSDIN", "aa_hexdigest": "5f7f774c368641cfdc22ec26a160be3f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0015AA695A", "query_cov": 0.8725314183123878, "subject_cov": 0.8983364140480592, "identity": 0.595, "score": 575.0, "evalue": 3.79e-199, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A2N1QA61" }, "genes": [ "gyrA" ], "truncated": "3-prime", "pseudogene": { "start": 537016, "stop": 538992, "observations": { "mutation": [ 538687 ], "stop": [ 538687 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L4", "query_cov": 1.0053859964093357, "subject_cov": 0.612691466083151, "identity": 0.986, "score": 1051.0, "evalue": 0.0, "gene_start": 1, "gene_end": 557, "reference_start": 1, "reference_end": 560, "reference_sequence": "MLFGEDDNNKKENKNFEEDNSHDLDEYTNFDDRIKKIFSDDDETKAKKIDEEDEEDEEEIPQDKEGYKVEPQLLDKEINGLKPANLSKVMKTSFIEYAMSVIVSRALPDARDGLKPVHRRILYGMSELGMFYTAPHKKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSLRYPLIDGHGNFGSVDGDEAAAMRYTEARMSKIAGAMVDGIKKNTVDFMDNYDATEKEPVVLPSRFPNLLVSGSSGIAVGMATNIPPHNLGETIDAVCALAKNPEINVFELMEYIQAPDFPTGGIIFNKKGLIEAYSTGRGSITIRSKAHIQELSNGKSKIIVTEIPYEVKKTEIMEKISELIKDKKIDGIADFRDESNRDGIRIVIDIKKSFVPEIVLNNLFKLTKLQSNYSFNMIALVNNEPKLLNLKACLEVYLKHQIEVVSRRLKFDLEKDRARAHILEGLKICIENIDAVIKIIKESKTDQEAQSKLSQAFNLSEIQTKAIVDMKLGRLTGLAIEKMNEELNEVHERIANYIRILGDHNLLINLIIEELQEIKNAYGDARRSDINWDELSSIDNEDLIPKRDIVITLTNNGYIKRLDIEEYREQRRGGVGVSTTKTYKDDAIQDILVANTHTDLLIFTDLAKVYRIRGHEVPVGTKQSKGIPIVNVIPSIQKNEKVIKILPINEYKQNEYLLTATKKGIVKKTPTILFESINRNGKIAFAIRDDDDKLIDALIVKDEDEIFIAASNNKICRFDSNEIRSMGRMAYGVKGIKLDDVEDNVVSVSSSNQGKYIFSLGKEGYGKLTLAAMFRKTRRGASGVYSLNEEKSGKLIYAATVHGVEDLIVLTSDGVAIRFSLKDVAITGRNTKGVKLVNLKGRTTSIVAATKILDDDLEDNDRKLSENEFKEIESDAKNNLNQKNE" }, "paralog": false, "identity": 0.9863842662632375, "score": 1272.0, "evalue": 0.0, "description": "Internal stop codon at 538687. Nonsense mutation around 538687.", "psc": { "uniref90_id": "UniRef90_D1J8L4", "gene": "gyrA", "product": "DNA gyrase subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_B3PN30", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L4", "EC:5.6.2.2", "UniRef:UniRef50_B3PN30", "COG:COG0188", "COG:L", "KEGG:K02469", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN30" ], "product": "DNA gyrase subunit A" } }, "id": "AHCKFKLKGB_915", "locus": "GCA013367975_00914" }, { "type": "cds", "sequence": "contig_1", "start": 538696, "stop": 539751, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03989.18" ], "nt": "TTGAGTAGCATCGACAATGAAGATTTGATTCCAAAACGTGATATTGTTATAACCTTGACAAATAATGGCTATATTAAAAGACTTGATATTGAGGAATATAGAGAACAAAGAAGAGGCGGAGTGGGTGTATCAACTACAAAAACATATAAAGATGATGCAATTCAAGATATATTAGTTGCAAATACCCACACGGACTTGTTGATATTTACAGATCTTGCCAAGGTTTATAGAATTAGAGGCCACGAAGTTCCCGTTGGCACCAAGCAATCAAAAGGTATCCCTATTGTTAATGTAATTCCTTCTATTCAAAAAAATGAAAAAGTTATTAAGATTTTACCAATAAATGAATACAAACAAAATGAATATTTACTAACAGCAACTAAGAAGGGGATTGTTAAAAAAACTCCAACTATTTTATTTGAAAGTATTAACAGAAACGGAAAAATTGCCTTTGCCATTCGTGATGATGATGACAAATTAATTGATGCATTGATTGTAAAAGATGAGGATGAAATCTTTATTGCTGCCTCAAATAATAAAATTTGCCGTTTTGATTCAAACGAAATCAGATCAATGGGAAGAATGGCCTATGGTGTTAAGGGAATTAAAATTGACGATGTTGAAGACAATGTTGTTTCTGTATCTTCTTCAAACCAAGGAAAATATATATTTAGTCTTGGGAAAGAAGGATATGGAAAATTGACACTTGCTGCAATGTTTAGAAAAACAAGACGTGGGGCAAGTGGGGTTTATTCATTAAATGAAGAAAAATCTGGTAAATTAATTTATGCTGCAACTGTTCACGGAGTTGAAGATCTGATAGTTTTAACAAGCGATGGTGTTGCAATTAGATTTTCTTTAAAAGATGTTGCAATAACCGGTAGAAATACTAAAGGTGTTAAATTAGTAAATCTAAAAGGAAGAACCACAAGCATTGTTGCAGCAACAAAAATTTTAGATGATGATTTAGAAGACAATGACAGAAAATTATCAGAAAATGAATTCAAAGAAATTGAATCAGATGCTAAAAATAACCTTAATCAAAAAAATGAATAA", "aa": "MSSIDNEDLIPKRDIVITLTNNGYIKRLDIEEYREQRRGGVGVSTTKTYKDDAIQDILVANTHTDLLIFTDLAKVYRIRGHEVPVGTKQSKGIPIVNVIPSIQKNEKVIKILPINEYKQNEYLLTATKKGIVKKTPTILFESINRNGKIAFAIRDDDDKLIDALIVKDEDEIFIAASNNKICRFDSNEIRSMGRMAYGVKGIKIDDVEDNVVSVSSSNQGKYIFSLGKEGYGKLTLAAMFRKTRRGASGVYSLNEEKSGKLIYAATVHGVEDLIVLTSDGVAIRFSLKDVAITGRNTKGVKLVNLKGRTTSIVAATKILDDDLEDNDRKLSENEFKEIESDAKNNLNQKNE", "aa_hexdigest": "55d88bc43c480ce905e50f0f43fba182", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03989.18", "name": "DNA_gyraseA_C", "length": 48, "aa_cov": 0.13675213675213677, "hmm_cov": 1.0, "evalue": 4.453086596498616e-57, "score": 184.67364501953125, "start": 14, "stop": 61 } ], "seq_stats": { "molecular_weight": 39147.20080000002, "isoelectric_point": 8.587204933166507 }, "id": "AHCKFKLKGB_916", "locus": "GCA013367975_00915" }, { "type": "cds", "sequence": "contig_1", "start": 540123, "stop": 540290, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAACTTTAAAGAACACAAAGCCACAACAGCGACCCTTAAACGTGGATCTTATTCTATACCATATACCAGAATTGATCCAAACAAATCAATATACAAAGAAGTTAATGATACTATAAAACGCTTAGAACAAAGTGGAAAATATGCCTCAAAAATTTCTGAATGA", "aa": "MSNFKEHKATTATLKRGSYSIPYTRIDPNKSIYKEVNDTIKRLEQSGKYASKISE", "aa_hexdigest": "061850484c6331e0555212d51b0fa3fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6325.082000000002, "isoelectric_point": 9.696899223327637 }, "id": "AHCKFKLKGB_917", "locus": "GCA013367975_00916" }, { "type": "cds", "sequence": "contig_1", "start": 540591, "stop": 541907, "strand": "-", "frame": 2, "gene": null, "product": "Lmp related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8L6", "UniRef:UniRef90_D1J8L6" ], "nt": "ATGAAAGAAAAAAGTAGAAAAATTGTTCTTCCATTAGCAATTTTTTGTGGCGGATTATCAATAGCTGCCATCGCTACCGTTGCAATAAGATCGTGCCAAAATAAAGTGGATAAAAAGACACAAAAAGAATCGCTCTTTAAAAAATTTAAAATTCTTCAAGAAGAATTGCAATCATTGCTAGAAAATAAAAACATTCAAGGAATTTATTCAATTGAAGAACAAAATGTCTTTGATAATTCCAAAATGATCAATATCGAATCGCCAATTGATAATATTCATCAAGAAATTTTTAAGATTAAAAGTTCAATTGATTCATTAAATGCAAAAATTCAAAAGCAAAGCATGCAAAAACTACTTTTTGCAGCAAAGAAACAGTTGGAAAATATTATTCATGCTATGAGGATATTGGACAATAAATTAATTGATAATGATGATGATATTCAAAAAACTAAGGAAGATTTAAATTCACAAATTATTAATGCATTAGAGGTTTATAACAAAAATAATATAGACGAAATAAATAATTTAAATAAATCATTAACTTTTAAAATTTCTGAAGTTGAAGATATATTGATCAAATTTAAGCAATCGAAAGAACAAGAATTTGAAAAATTACAAAAAACTAGAAAATTAGTTGAAGAATTTTTGGCCAAAGATGTTGTTAAATCTGATTATTATGCAAATATAGTTAGAAATCTTAATAGAGAAGTTGCAAGACGAGGAAACGTTTCAATTAGTTCTAATAAATCAGAAATAGTAGCTACAAATATTCACTTGAACGAAATTTTAAAACAAAGTAAAAAATTTGTTGAAGATGATATTCAAAAATTAAGGGATAGCTTAATTTCTTTAATTACAGAAGCAAAAGAATATATTGAAAATAAAATGCGCCCTAGAGATAAATACAATGCATACATTGAAAGAATAGAATGGTCTATTAGGAAACATAATATTAGTACTCTACATACAAGAGAAGAAATAATACAAGTAATTAATGAAATTTCAGTGGCATATCTTGAGGCTAAAAAATTATTAATAAATAATAGTCTAGATGATGTAAATAATAGTTTACAAAAATTTAATAAGCTAGATAGTAGTGTAAAAGATCCAGATTTTTTAGGTGTAACACAACAACTAAATGAAATAAAACAAAAGGCATTGCAAGAATTAACAAATATTAAAAATAATTCTAGTCAATTGGCAACTCTTGCAGAAGCGTCAAAATATGAAAATGATATACAAGAAATCCAAGAAAAATTTGTTAAAGATTTAATAAAAATATTTGATGGAAATATAAATACTGATAGGATTAATTGA", "aa": "MKEKSRKIVLPLAIFCGGLSIAAIATVAIRSCQNKVDKKTQKESLFKKFKILQEELQSLLENKNIQGIYSIEEQNVFDNSKMINIESPIDNIHQEIFKIKSSIDSLNAKIQKQSMQKLLFAAKKQLENIIHAMRILDNKLIDNDDDIQKTKEDLNSQIINALEVYNKNNIDEINNLNKSLTFKISEVEDILIKFKQSKEQEFEKLQKTRKLVEEFLAKDVVKSDYYANIVRNLNREVARRGNVSISSNKSEIVATNIHLNEILKQSKKFVEDDIQKLRDSLISLITEAKEYIENKMRPRDKYNAYIERIEWSIRKHNISTLHTREEIIQVINEISVAYLEAKKLLINNSLDDVNNSLQKFNKLDSSVKDPDFLGVTQQLNEIKQKALQELTNIKNNSSQLATLAEASKYENDIQEIQEKFVKDLIKIFDGNINTDRIN", "aa_hexdigest": "c0d70bb21be73378066e5c90f8dc8315", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_D1J8L6", "query_cov": 1.0, "subject_cov": 0.9106029106029107, "identity": 0.9179999999999999, "score": 721.0, "evalue": 1.78e-259, "valid": true, "product": "Lmp related protein", "uniref50_id": "UniRef50_D1J8L6" }, "pscc": { "uniref50_id": "UniRef50_D1J8L6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8L6" ], "product": "Lmp related protein" }, "genes": [], "id": "AHCKFKLKGB_918", "locus": "GCA013367975_00917" }, { "type": "cds", "sequence": "contig_1", "start": 542075, "stop": 542206, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCCAATAATAGCAAAAACTCTTATTGAAATTCCAATATATACAGCAGTTATTTTTGTACTTTATCCAATAATAATTATGATTAGACAAAAAATATTATTTTACACAAGTAAAATATATTCATATTAA", "aa": "MIPIIAKTLIEIPIYTAVIFVLYPIIIMIRQKILFYTSKIYSY", "aa_hexdigest": "eea31676119cfefb6be72b88549ea62e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5108.317999999999, "isoelectric_point": 9.522770118713382 }, "id": "AHCKFKLKGB_919", "locus": "GCA013367975_00918" }, { "type": "cds", "sequence": "contig_1", "start": 542268, "stop": 542519, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCAAATGGTCCTCAAAATCATCTACACAACATTAAAGTGAAAAACACAACCAATAAAATGCTAAATAAAAGTACAACATTGCTTTTTACAGAAAATTTTCTATTTTTTATAAATAAAGTTAAAAAAGTTATAGAAGATATTCAAATAAATGCCAATCCTACAATAGCAATAGACAAAACAATTCTGATTGGTATTAATTTGGTGCGTTTTACTGCTGTTTCATTTATTGGCAAATTGTTACCGTGA", "aa": "MYANGPQNHLHNIKVKNTTNKMLNKSTTLLFTENFLFFINKVKKVIEDIQINANPTIAIDKTILIGINLVRFTAVSFIGKLLP", "aa_hexdigest": "a33a9996d53fc2eb5ee8ec889651e182", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9399.031999999992, "isoelectric_point": 9.998482322692869 }, "id": "AHCKFKLKGB_920", "locus": "GCA013367975_00919" }, { "type": "cds", "sequence": "contig_1", "start": 542553, "stop": 542777, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAAAACTAAATCCAATTATTCATGTAATCTTTTGTTTAGCATACATTAATCTTAATAATCAGCCACTTATTAAAGCAATAAAAACAGGCACTAACGCATATTCAATCATTCATGTACTTATTCCTCTAATAACCTGGTTTAGCGTATCACATAATATACCTAAAAAGGCACCCTTTCACGGTCCTAATGCCAACCCAAAAATAATGAATACTGCATGA", "aa": "MLKKLNPIIHVIFCLAYINLNNQPLIKAIKTGTNAYSIIHVLIPLITWFSVSHNIPKKAPFHGPNANPKIMNTA", "aa_hexdigest": "286c2b3daeae1eff76a91d5d85a7d658", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8271.855099999993, "isoelectric_point": 10.111173057556147 }, "id": "AHCKFKLKGB_921", "locus": "GCA013367975_00920" }, { "type": "cds", "sequence": "contig_1", "start": 542735, "stop": 542938, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAATTTATTCAAAAAATTAGACGTATTAAAAAAACAAAACAACATAAATTAACAGTTTTTGAAATTTCTTTATTTGCTTTACTTTTAGCTATTTATATAATTGCTGCAATCTTAGAAAGATTTGTTTTCAAAGGAATAATGAATATTAATATTACTCATGCAGTATTCATTATTTTTGGGTTGGCATTAGGACCGTGA", "aa": "MFEFIQKIRRIKKTKQHKLTVFEISLFALLLAIYIIAAILERFVFKGIMNINITHAVFIIFGLALGP", "aa_hexdigest": "ff53a0c1726f4703c5c0e7233c126d13", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7763.474099999994, "isoelectric_point": 10.377105140686034 }, "id": "AHCKFKLKGB_922", "locus": "GCA013367975_00921" }, { "type": "cds", "sequence": "contig_1", "start": 543113, "stop": 543340, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCTTTTGATTCTGACTATGAAGAATTTATGAAGGGTTCAATTGTTGCAGATTTGAAGAATACAGACTATACCGGACATGCAGGTTCTTGTTCAGCAGCAATGTTCTTAAAAGAATTTACAAAAGGAATTGAATATATTCACTTAGATGTTGCAGGAACTAATGATATTGATGAAAAACCAATGTTTGCAATGACAAAAACTTTGACAGAATTAGTGTTGAAATAA", "aa": "MPFDSDYEEFMKGSIVADLKNTDYTGHAGSCSAAMFLKEFTKGIEYIHLDVAGTNDIDEKPMFAMTKTLTELVLK", "aa_hexdigest": "d7f4f7412216ed7607c491ffc4bc2a8d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 59, "aa_cov": 0.7866666666666666, "hmm_cov": 0.9491525423728814, "evalue": 7.1047293476586465e-15, "score": 50.34300994873047, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 8373.472299999996, "isoelectric_point": 4.60369701385498 }, "id": "AHCKFKLKGB_923", "locus": "GCA013367975_00922" }, { "type": "cds", "sequence": "contig_1", "start": 543410, "stop": 544027, "strand": "-", "frame": 3, "gene": null, "product": "M17 family metallopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201A65A" ], "nt": "TTGGGTATTACCCCTCCTAATGAATTAAATTCTGAACAATTAGCTAAAATAGTTGTTGAAGACTTTAAAGAATATAAAAATCTATCAGTTAAAGTTCTTGAAAAGAAAGAAATTGAAAAATTGGGTATGGGATTATTACTATCTGTGAATAGAGGTTCAGTTTATGAACCTAGAGTTGTAATAATCGAATATAAAGGAAATCCTGATTCAAATCAAAAAACAGTTTTAATTGGTAAAGGAATTACCTTTGACTCTGGCGGTTACAACATTAAAACAGGCCGCAACATGTTAGGAATGAAATATGATATGTCAGGTTCTGCAATAGTTGCATCCGTTTTGAAATCTGTTGCCCAATTAAAACCAAAAACAAATGTATCAGCAATTATGTGCATTACTGACAATAGAGTTAATGGAGATGCATCGTTGCCTGATTCAGTTTGGACCTCAATGAGCGGAAAGACTGTTGAAATAAATAATACTGACGCTGAAGGTAGATTAGTTATGGCTGATGGCTTGTACTATGGAGCTGATGTATTAAAAGCAACAAGATTAATTGATGTTGCCACATTAACTGGTGCAATGGTTGCAGCCCTAGGAGACACATATACAGGCATTTGA", "aa": "MGITPPNELNSEQLAKIVVEDFKEYKNLSVKVLEKKEIEKLGMGLLLSVNRGSVYEPRVVIIEYKGNPDSNQKTVLIGKGITFDSGGYNIKTGRNMLGMKYDMSGSAIVASVLKSVAQLKPKTNVSAIMCITDNRVNGDASLPDSVWTSMSGKTVEINNTDAEGRLVMADGLYYGADVLKATRLIDVATLTGAMVAALGDTYTGI", "aa_hexdigest": "3394784bffc5a402b5bc81c8a5fdddbf", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002201A65A", "query_cov": 0.9365853658536586, "subject_cov": 0.8170212765957446, "identity": 0.594, "score": 222.0, "evalue": 2.96e-70, "valid": false, "product": "M17 family metallopeptidase", "uniref50_id": "UniRef50_UPI002201A65A" }, "pscc": { "uniref50_id": "UniRef50_UPI002201A65A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201A65A" ], "product": "M17 family metallopeptidase" }, "genes": [], "id": "AHCKFKLKGB_924", "locus": "GCA013367975_00923" }, { "type": "cds", "sequence": "contig_1", "start": 544037, "stop": 544486, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAATTAAACAATTATCAACAAAACGCAATGATCATTTGTTATTGAAAGCAATATTTGAAGATGAAATTTTGCCAGAATTTTTAGTTAAAGAAACAGAAGCAATAACAGATTTTATTGATAAAAAAGTTTCGTATATTTATCTAGGCAAAAAGCAAGAATTACAATACAATTCTTTATATTCATTGGCACAAGATTTAGCATTGAATGCTGCCTACGATTATCAAATTGATCTTTCTTCTTTTGTAACAGAAAATTTAACAATTAATGATGTAGTTGATGCATTCACAAAAGGAATAAATTTCTCAGCTGCAAAGTTATACAATAAAAAGACATTTACATATAAAGAAAATCCAAACAACTTAGAATTGTTTATAGAACAAGCGTCTCAATCAGTAATTGATTCATTCAATAAAGCAAAAATATTAATTGAATCTCAAAACTGA", "aa": "MQLIKQLSTKRNDHLLLKAIFEDEILPEFLVKETEAITDFIDKKVSYIYLGKKQELQYNSLYSLAQDLALNAAYDYQIDLSSFVTENLTINDVVDAFTKGINFSAAKLYNKKTFTYKENPNNLELFIEQASQSVIDSFNKAKILIESQN", "aa_hexdigest": "000609b166c0d558e6bb08c1c7d928c0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17161.293199999986, "isoelectric_point": 4.910002326965332 }, "id": "AHCKFKLKGB_925", "locus": "GCA013367975_00924" }, { "type": "cds", "sequence": "contig_1", "start": 544500, "stop": 544733, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCTCTACATGATGATTATGATAAACCAAATAAAGAATCTAAAGTAGCTGATCTTAATAATTACAACAACAGTGAAAAATCAGATTGTAATACAGCAGCAATGTTCTTAAAACATTTTACAAATAATGTTGACTATATTCATTGCGATATTGCAGGAACCGCTGACAATAAAGGAGTAGGACTAGGAATTTTAGTTTCTACCTTTGTTGAATTAGCCGCAAATCAAAAATAA", "aa": "MPLHDDYDKPNKESKVADLNNYNNSEKSDCNTAAMFLKHFTNNVDYIHCDIAGTADNKGVGLGILVSTFVELAANQK", "aa_hexdigest": "fe1abfe2770c91cf8972a0375caca55b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 58, "aa_cov": 0.7532467532467533, "hmm_cov": 0.9655172413793104, "evalue": 4.473365602797555e-15, "score": 51.002933502197266, "start": 1, "stop": 58 } ], "seq_stats": { "molecular_weight": 8490.333599999998, "isoelectric_point": 5.05994358062744 }, "id": "AHCKFKLKGB_926", "locus": "GCA013367975_00925" }, { "type": "cds", "sequence": "contig_1", "start": 544803, "stop": 545864, "strand": "-", "frame": 2, "gene": "pepB", "product": "Aminopeptidase (Leucine aminopeptidase)", "db_xrefs": [ "COG:COG0260", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZXA3", "UniRef:UniRef90_D1J8L9" ], "nt": "ATGAATTCTATTTATTATGAAACCAAACGTAATAAAGATATGCTTTTAACAGCAGTTTATGAAGGCTCAAACGCTAGTTTAAATATTGCCAAGAAAGATAATCATATTACAGAAGATTTTAAGAAAAATGAAGCATTTATTTACATATCAAAAACTGTAGATAACTACTTTAGTTTTTTAAAAGTAGTTGACAAAATTCTAAGTTCAAATAGAAGAAATTATCAAATTAATATTGCTTCTTTTGTTAAAGAAAATGTTGTGTCAACAGAAGAAGTTTTTAGAGCATTTGTATCTAAATTAGCATTTAAAAATGCAAATCTTTATTCAGCAAAAACCAAGGAAAACAAACAAGAAAAGACAATAACATTGTTTTATGCTGATAAATCGTATGAAGAATTAGTTGAAAGATTGTCAATAATTCTTGAAGCAACAAATAAAGCCCGTAGTTTGCAAATGACACCCCCTAATGTTGCAACTAGTGAATATATCGCTAAGGTAGTAAAAGAAGATTTAGAAAACATTCCTGGACTATCAATAAAAGTTTTAGATCGTGATAACATAAAGAAAAATAACATGGGACTTATGTTGGCAGTAAATTCGGGTTCTTCTTATGAACCAAGAGTTTTGATTGCTGAATATAATGGCAACCCCGAAAGTAAAGAAAAATATGTTTATGTAGGAAAAGGAATTACATTTGACACTGGTGGATATAACACCAAAGGATACTACATGGATGGTATGAAATTTGACATGTCTGGCTCTGTTATTGTTGCATATGCAGTTAAAGCAATTGCTCAACTAAAATTAAAAGCAAACGTAGCAGCAATAATGATGATTACAGATAACGCAATAGATACACACCCAACAATGCCTGAATCAGTAATTAAATCAATGAGTGGTTTAACAGTTGAAATTACTGATACTGATGCTGAAGGAAGATTAGTGTTGGCTGATGGCTTATACTACGGTGCTTCGAAATTGAATGCTTCATTATTAATTGACGCTGCAACTTTAACCGGTTCAATGGAAAGAGCATTAGGAAAGACATATTCAGGAATTTGA", "aa": "MNSIYYETKRNKDMLLTAVYEGSNASLNIAKKDNHITEDFKKNEAFIYISKTVDNYFSFLKVVDKILSSNRRNYQINIASFVKENVVSTEEVFRAFVSKLAFKNANLYSAKTKENKQEKTITLFYADKSYEELVERLSIILEATNKARSLQMTPPNVATSEYIAKVVKEDLENIPGLSIKVLDRDNIKKNNMGLMLAVNSGSSYEPRVLIAEYNGNPESKEKYVYVGKGITFDTGGYNTKGYYMDGMKFDMSGSVIVAYAVKAIAQLKLKANVAAIMMITDNAIDTHPTMPESVIKSMSGLTVEITDTDAEGRLVLADGLYYGASKLNASLLIDAATLTGSMERALGKTYSGI", "aa_hexdigest": "ccbe4ab673cc3ffd72b47ea887154ca3", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "pepB" ], "truncated": "5-prime", "pseudogene": { "start": 544500, "stop": 545864, "observations": { "mutation": [ 544739, 544784, 544805 ], "stop": [ 544739, 544784, 544805 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L9", "query_cov": 1.0, "subject_cov": 0.7775330396475771, "identity": 0.997, "score": 670.0, "evalue": 2.78e-241, "gene_start": 1, "gene_end": 353, "reference_start": 1, "reference_end": 353, "reference_sequence": "MNSIYYETKRNKDMLLTAVYEGSNASLNIAKKDNHITEDFKKNEAFIYIPKTVDNYFSFLKVVDKILSSNRRNYQINIASFVKENVVSTEEVFRAFVSKLAFKNANLYSAKTKENKQEKTITLFYADKSYEELVERLSIILEATNKARSLQMTPPNVATSEYIAKVVKEDLENIPGLSIKVLDRDNIKKNNMGLMLAVNSGSSYEPRVLIAEYNGNPESKEKYVYVGKGITFDTGGYNTKGYYMDGMKFDMSGSVIVAYAVKAIAQLKLKANVAAIMMITDNAIDTHPTMPESVIKSMSGLTVEITDTDAEGRLVLADGLYYGASKLNASLLIDAATLTGSMERALGKTYSGIWSTSEQRWEDFNKAAKIAHEKVWRMPLHDDYDKPNKESKVADLNNYNNSEKSDCNTAAMFLKHFTNNVDYIHCDIAGTADNKGVGLGILVSTFVELAANQK" }, "paralog": false, "identity": 0.986784140969163, "score": 871.0, "evalue": 0.0, "description": "Internal stop codon at 544739, 544784, 544805. Nonsense mutation around 544739, 544784, 544805.", "psc": { "uniref90_id": "UniRef90_D1J8L9", "gene": "PepB", "product": "Aminopeptidase (Leucine aminopeptidase)", "uniref50_id": "UniRef50_A0A448ZXA3", "cog_id": "COG0260", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L9", "UniRef:UniRef50_A0A448ZXA3", "COG:COG0260", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXA3" ], "product": "Cytosol aminopeptidase" } }, "id": "AHCKFKLKGB_927", "locus": "GCA013367975_00926" }, { "type": "cds", "sequence": "contig_1", "start": 546027, "stop": 546317, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_073673955.1", "SO:0001217", "UniParc:UPI00093BBF14", "UniRef:UniRef100_UPI001939D1DF", "UniRef:UniRef50_A0A454CA46", "UniRef:UniRef90_UPI001594684D" ], "nt": "ATGAAGAAGAGCAAGAAAATAATTTTTTCTATTTTAGGAGCAAGTTCTCTAATTTCATTACCATTAGTAGCAATATCATGTTCAAAATTGACATCAAAGCAAAAACAAGTTAAATCAAAAATAAAGCAATTACTTGATATGGCAAAAAATATACCATCTGCCGGTGAAATCATGAGCAAATATGAAAACATGATAAACGATGATGAACTTGCAAAATTAAGTGATCAACAATGCGACGCAATCTTATTCAGTATAGATTTAGTTATTAATCAACTTAAAAATATTAAATAA", "aa": "MKKSKKIIFSILGASSLISLPLVAISCSKLTSKQKQVKSKIKQLLDMAKNIPSAGEIMSKYENMINDDELAKLSDQQCDAILFSIDLVINQLKNIK", "aa_hexdigest": "915b1ab7ab7a84379a73d5cda42d71fd", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00093BBF14", "ncbi_nrp_id": "WP_073673955.1", "uniref100_id": "UniRef100_UPI001939D1DF", "db_xrefs": [ "SO:0001217", "UniParc:UPI00093BBF14", "RefSeq:WP_073673955.1", "UniRef:UniRef100_UPI001939D1DF" ] }, "ips": { "uniref100_id": "UniRef100_UPI001939D1DF", "uniref90_id": "UniRef90_UPI001594684D" }, "psc": { "uniref90_id": "UniRef90_UPI001594684D", "uniref50_id": "UniRef50_A0A454CA46" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA46" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "AHCKFKLKGB_928", "locus": "GCA013367975_00927" }, { "type": "cds", "sequence": "contig_1", "start": 546395, "stop": 546526, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAAACAAATAATAGTTTATGCACCACATCCTTCACCTCTTGCTGCATATAAAGGATTTTTTGGTTCAAATATTTTTAAAAAAATAAATTTTAAGCTTAAATCATTATCAAAAGATGAAATCGATTGA", "aa": "MSKQIIVYAPHPSPLAAYKGFFGSNIFKKINFKLKSLSKDEID", "aa_hexdigest": "d93dbe5626116da2e382c35d1583ef6d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4872.681700000001, "isoelectric_point": 9.6994779586792 }, "id": "AHCKFKLKGB_929", "locus": "GCA013367975_00928" }, { "type": "cds", "sequence": "contig_1", "start": 546713, "stop": 547030, "strand": "-", "frame": 3, "gene": "ung", "product": "uracil-DNA glycosylase", "db_xrefs": [ "COG:COG0692", "COG:L", "EC:3.2.2.27", "GO:0004844", "GO:0006284", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_D1J8M1", "UniRef:UniRef90_D1J8M1" ], "nt": "ATGTCAAAATTTGCTTTTTTAGAATTTTTAAAAAAAGAAACTTCAAAACCATATTTTGAAGTCTTAAAAAAATTAACAATGTTGCCTAATGTAACGCCAAAGCCAAATAGAGTATTTTTTGCATTTAAAAATTTTGATTTTGGCAACCTAAAGGTTATTATCATTGGTCAAGATCCTTATCCTAACAAAGGCGATGCAGATGGTATGTGTTTTTCAAGCAATGCAACAAAATGCCCGGCATCTTTGAAGAATATATTTGTTGAAATAAAAAACGAATATCCAAATTCAACGTTTGAAACTTTTTCACTAAATTATTGA", "aa": "MSKFAFLEFLKKETSKPYFEVLKKLTMLPNVTPKPNRVFFAFKNFDFGNLKVIIIGQDPYPNKGDADGMCFSSNATKCPASLKNIFVEIKNEYPNSTFETFSLNY", "aa_hexdigest": "98e855a1eca40780f40a19c521d256bb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ung" ], "truncated": "5-prime", "pseudogene": { "start": 546410, "stop": 547030, "observations": { "mutation": [ 546634, 546715 ], "stop": [ 546634, 546715 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M1", "query_cov": 1.0, "subject_cov": 0.4861111111111111, "identity": 0.971, "score": 213.0, "evalue": 1.94e-68, "gene_start": 1, "gene_end": 105, "reference_start": 1, "reference_end": 105, "reference_sequence": "MSKFVFLEFLKKETSKPYFEVLKKLTMLPNVTPKPNRVFFAFKNFDFDNLKVIIIGQDPYPNKGDADGMCFSSNATKCPASLKNIFIEIKNEYPNSTFETFSLNYWKNQGVLLLNSILTNIEGKTLAHKNLGWELFNQNLLTEINSCYSNIIYLILGNNAKDFIKNIDMSKQIIVYAPHPSPLAAYKGFFGSDVFKKINFKLKSLSKDGIDWSTKK" }, "paralog": false, "identity": 0.9563106796116505, "score": 401.0, "evalue": 2.56e-145, "description": "Internal stop codon at 546634, 546715. Nonsense mutation around 546634, 546715.", "psc": { "uniref90_id": "UniRef90_D1J8M1", "gene": "Ung", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_D1J8M1", "cog_id": "COG0692", "cog_category": "L", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0006284" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M1", "EC:3.2.2.27", "UniRef:UniRef50_D1J8M1", "COG:COG0692", "COG:L", "KEGG:K03648", "GO:0004844", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M1" ], "product": "Uracil-DNA glycosylase" } }, "id": "AHCKFKLKGB_930", "locus": "GCA013367975_00929" }, { "type": "cds", "sequence": "contig_1", "start": 547033, "stop": 547338, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02911.23" ], "nt": "TTGTTTGAAATTTACAACGGCCAATATAATCGAGTGGCTCAGGATGAATCTAAAGTAACATTTGCTCCTAAGATATTAAATGGTATGGAAAGAATTGATCAAAGCGAAAATCTTTCAAGTGTTATAAACAAAATCAGAGCTTTTAATGACGAACCAGGTGCTTATTTAATTTATCAAAATAAAAGATTAAAAATCTTCCGGGCTTCAACAACAAAAATTAGTACTCCATTAAAAATAAAATTTAATGATGGATATTTATATTTAATAGAATATCAATTTGAAGGGAAAAAAAGAGTAAGAGTTTAA", "aa": "MFEIYNGQYNRVAQDESKVTFAPKILNGMERIDQSENLSSVINKIRAFNDEPGAYLIYQNKRLKIFRASTTKISTPLKIKFNDGYLYLIEYQFEGKKRVRV", "aa_hexdigest": "296f09a513f9b9f2493cc814ceee386e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02911.23", "name": "Formyl_trans_C", "length": 86, "aa_cov": 0.7128712871287128, "hmm_cov": 1.0, "evalue": 1.0800886714676305e-12, "score": 43.39727783203125, "start": 28, "stop": 99 } ], "seq_stats": { "molecular_weight": 11876.504999999986, "isoelectric_point": 9.616571617126464 }, "id": "AHCKFKLKGB_931", "locus": "GCA013367975_00930" }, { "type": "cds", "sequence": "contig_1", "start": 547339, "stop": 547950, "strand": "-", "frame": 1, "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "db_xrefs": [ "COG:COG0223", "COG:J", "EC:2.1.2.9", "GO:0004479", "GO:0005737", "GO:0071951", "SO:0001217", "UniRef:UniRef50_B3PNE5", "UniRef:UniRef90_D1J8M2" ], "nt": "TTGCTTGAAATACAAGATAATAAGCTTATAAAAAATAATTTATTTTATAATTTTGATATGAAAAATATAAATAACAAAATTAATTTAGTTTTAGCGGGAACTGGACAATTTTCCGCTAAAACTTTTGAACCTCTTTTAAATGATGAAAGGTTCAATGTTTTGGCATTTATTTCTCAACCAAATAGGAAACTAGACCGAAACAAAAATGTAATTGAAACAGAAATTTTTAAACTTGCCAAACAGTATGATATAAAAGTTTTTCAACCTCAAAAAATAAAAGAAATTTTGCCTGAACTTAGTGCAATGGATTTTGACTTTTTTATTACGGCTTCATTCGGACAATTTATACCTGATTCAATATTAAAATTACCTAAAAAAATGCCCTTGAACGTTCACGGAAGTTTGCTTGAAAAATATCGTGGGGCTGCGCCTGTGCAATATGCATTACTCAATGGTGATATTGAAACAGGAATAACATTAATTGAAATGGTAAAACAAATGGATGCGGGGGATATCCTAAAATCCTCAAAAGTAAAAATTGATTCTATTGATACAGCAATTGAAGTATTTGATAAATTAGCAGAAGCAGCCTCAAAAAATCTTGGTAATTGA", "aa": "MLEIQDNKLIKNNLFYNFDMKNINNKINLVLAGTGQFSAKTFEPLLNDERFNVLAFISQPNRKLDRNKNVIETEIFKLAKQYDIKVFQPQKIKEILPELSAMDFDFFITASFGQFIPDSILKLPKKMPLNVHGSLLEKYRGAAPVQYALLNGDIETGITLIEMVKQMDAGDILKSSKVKIDSIDTAIEVFDKLAEAASKNLGN", "aa_hexdigest": "c94921f107dbc98e5c0a34a2ed9cfe39", "start_type": "TTG", "rbs_motif": null, "genes": [ "fmt" ], "truncated": "5-prime", "pseudogene": { "start": 547036, "stop": 547950, "observations": { "mutation": [ 547341 ], "stop": [ 547341 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M2", "query_cov": 1.0, "subject_cov": 0.6655737704918033, "identity": 0.985, "score": 389.0, "evalue": 4.34e-135, "gene_start": 1, "gene_end": 203, "reference_start": 1, "reference_end": 203, "reference_sequence": "MLEIQDNKFIKNNLFYNFDMKNINNKINLVLAGTGQFSAKTFEPLLNDERFNVLAFISQPNRKLDRNKNVIETEIFKLAKQYDIKVFQPQKIKEILPELSAMDFDFFITASFGQFIPDSILKLPKKMPLNVHGSLLEKYRGAAPVQYALLNGDIETGITLIEMVKQMDAGDILKSSKVKIDSIDTAIEVFDKLADVASKNLGNWLFEIYNGQYNRVAQDESKVTFAPKILNGMERIDQSENLSSVVNKIRAFNDEPGAYLIYQNKRLKIFRASTTKISTPLKIKFNDGYLYLIEYQFEGKKRVRV" }, "paralog": false, "identity": 0.9802631578947368, "score": 586.0, "evalue": 2.39e-215, "description": "Internal stop codon at 547341. Nonsense mutation around 547341.", "psc": { "uniref90_id": "UniRef90_D1J8M2", "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "ec_ids": [ "2.1.2.9" ], "uniref50_id": "UniRef50_B3PNE5", "cog_id": "COG0223", "cog_category": "J", "go_ids": [ "GO:0004479", "GO:0005737", "GO:0071951" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M2", "EC:2.1.2.9", "UniRef:UniRef50_B3PNE5", "COG:COG0223", "COG:J", "GO:0004479", "GO:0005737", "GO:0071951" ] }, "pscc": { "uniref50_id": "UniRef50_B3PNE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PNE5" ], "product": "Methionyl-tRNA formyltransferase" } }, "id": "AHCKFKLKGB_932", "locus": "GCA013367975_00931" }, { "type": "cds", "sequence": "contig_1", "start": 547991, "stop": 548392, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGCAAATAGCAAACAAGATCTAATTGCAAGGTTCACAATTTTGAAATTCTTGAATCAACAAAAAGATTTATTTCTAGCAAATAATAATGCATTGCCAGCCAATACACCAATCGGAATAAAATATAGTCAATATAACATATTTAATATTAACAACGAATATAAGACACTAAATATTTTAAATGTTTATCAACCTGTAATTTTAAAATATTCAACCATTGCTGAAGCATTCAATCAAAATGGTTTAGACCAATTGTTAAAGAATAATGAATTTGCAAACTATTTTAAATTTTTAGACAGTAGTTACATTCAATTTTCAAATAACGTTTTAAGCAATGATAGTTTAACAAAAATAGGTTCAAAAACAGAAAATTTATTGAACTTCATATTGCCTTGA", "aa": "MKEANSKQDLIARFTILKFLNQQKDLFLANNNALPANTPIGIKYSQYNIFNINNEYKTLNILNVYQPVILKYSTIAEAFNQNGLDQLLKNNEFANYFKFLDSSYIQFSNNVLSNDSLTKIGSKTENLLNFILP", "aa_hexdigest": "bdb8d8c68d1292160384ca6cb8008a0d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15296.218499999988, "isoelectric_point": 8.776935386657716 }, "id": "AHCKFKLKGB_933", "locus": "GCA013367975_00932" }, { "type": "cds", "sequence": "contig_1", "start": 548636, "stop": 548953, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTTGCGAATATTTTAAAACAGGTCGTTCTTTAGGAACAATTAATAATATAAAAATTGGAGAAGCAAGTCTAAATATAACTTTATATGGAAGTAGTATTAAAGAAATTTTTTCAAGGATATTTTGGCCTGGTTACATATTTAGCTTTGTCATTATAATTTTTGTAAGTATATCTGTTGGATTGTTCATTATAAGCGTTTCAAAATCAGTCTTAACTATTCAAGCAATCGGTTGCTCGCTATTAATAATCACAATGTTCTTATCGGGTCAAGTGTTGCCTATTTCTCAAATTTCAAATATAAAAGGAATGTGA", "aa": "MFFCEYFKTGRSLGTINNIKIGEASLNITLYGSSIKEIFSRIFWPGYIFSFVIIIFVSISVGLFIISVSKSVLTIQAIGCSLLIITMFLSGQVLPISQISNIKGM", "aa_hexdigest": "1d73e91d4b5879eb6dd364b549a4c19c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11597.770399999989, "isoelectric_point": 9.390352058410645 }, "id": "AHCKFKLKGB_934", "locus": "GCA013367975_00933" }, { "type": "cds", "sequence": "contig_1", "start": 549042, "stop": 549167, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGAAGCACCAATTCTTTTTAAAATTGTTGATTTTTTAAACTCAAATATTGCCGTTGGCAGTGAAATACAGGCTATTGCTACCGGCCCAATGGTTATTGTGCTTCCTAAAATCATTTCATAA", "aa": "MLEAPILFKIVDFLNSNIAVGSEIQAIATGPMVIVLPKIIS", "aa_hexdigest": "365c3ba57a6f825defea4261b7090197", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4367.260099999999, "isoelectric_point": 4.677076148986816 }, "id": "AHCKFKLKGB_935", "locus": "GCA013367975_00934" }, { "type": "cds", "sequence": "contig_1", "start": 549342, "stop": 550187, "strand": "-", "frame": 2, "gene": "rbbA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1131", "COG:JV", "RefSeq:WP_175933568.1", "SO:0001217", "UniParc:UPI00158D095E", "UniRef:UniRef100_UPI00158D095E", "UniRef:UniRef50_D1J8M4", "UniRef:UniRef90_D1J8M4" ], "nt": "GTGGAAAATGAAAAAGCTTGCAAAAATAAAAGTCTAGAAGTAATAGACAATTTTTTAAGTAATTTAGAAGATCACGAAAAAAACACAATTAAAGCAAATTCAATTGAAATGCGCCAATACAATTTAAAAAAGTTGGTAAAAATTAAAAATTTGACAAAAGAATTCAAAATAAAAAAGGAAAAAATAAAAGTTGTAGATAATTTAAATCTAGAAATTTATAAGAATCAAAACATTGCTCTATTAGGCGGAAATGGAGCAGGAAAAACTACAACAGTTGAAATGTTTATAGGAATTTTAAAACCAACTTCTGGTAACATTGAATATTATTTTAATGACGGATTAAACCATAACGAAAGTGCACAAACAAATATTGGAATTCAATTTCAAGATTCTTCTTATCCTCAAGGCTTATCGGTTAACGACGTTATTTATTCAATGAATAAAATCTATGGCAATAAAAGTTCAGCAGAAGAATTACAAAAACTTATAAAGATTTTTGGGGTTGATGAATTTATAAATAATAAAGCTTCTTCTTTAAGTGGTGGTCAACATCAAAGACTTAATGCATTGCTTGCAATAATTAACAAACCAAAGTTGTTAATATTAGATGAATTAAGCACAGGCCTTGACATAAAAATAAAAACAAGATTAGTATCATTTTTGAATGAATATATCAAAGAAATTGATTGTACTTTGATTTTAATATCTCATGATATAAATGAAATTGAAATTTTGGCAGATAGAATTATTATTATGAATAAAGGCAGAATTGTCCTTGATGCTTTGAAAAGCGAAATAATTGAAAAATATGGATCAGTATCTCAATGTCTTAAGGAATATATTTAA", "aa": "MENEKACKNKSLEVIDNFLSNLEDHEKNTIKANSIEMRQYNLKKLVKIKNLTKEFKIKKEKIKVVDNLNLEIYKNQNIALLGGNGAGKTTTVEMFIGILKPTSGNIEYYFNDGLNHNESAQTNIGIQFQDSSYPQGLSVNDVIYSMNKIYGNKSSAEELQKLIKIFGVDEFINNKASSLSGGQHQRLNALLAIINKPKLLILDELSTGLDIKIKTRLVSFLNEYIKEIDCTLILISHDINEIEILADRIIIMNKGRIVLDALKSEIIEKYGSVSQCLKEYI", "aa_hexdigest": "ffc4b5e60a20264f6ccb1eba3db378eb", "start_type": "GTG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158D095E", "ncbi_nrp_id": "WP_175933568.1", "uniref100_id": "UniRef100_UPI00158D095E", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D095E", "RefSeq:WP_175933568.1", "UniRef:UniRef100_UPI00158D095E" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D095E", "uniref90_id": "UniRef90_D1J8M4" }, "psc": { "uniref90_id": "UniRef90_D1J8M4", "gene": "RbbA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_D1J8M4", "cog_id": "COG1131", "cog_category": "JV" }, "pscc": { "uniref50_id": "UniRef50_D1J8M4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M4" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "rbbA" ], "id": "AHCKFKLKGB_936", "locus": "GCA013367975_00935" }, { "type": "cds", "sequence": "contig_1", "start": 550294, "stop": 550896, "strand": "-", "frame": 1, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "db_xrefs": [ "COG:COG0344", "COG:I", "EC:2.3.1.15", "EC:2.3.1.275", "GO:0005886", "GO:0006654", "GO:0008374", "KEGG:K08591", "SO:0001217", "UniRef:UniRef50_D1J8M5", "UniRef:UniRef90_D1J8M5" ], "nt": "TTGGCAATAATAATAACCAAATTAAGAGGAAATGATATAAGAAAAATTGGCTCGCAAAATCCTGGAGCAACAAATGCATTAAGAAATTTTGGAATAAAATTTGCAGCATTAGTTTTTGCTTTTGACATTTTTAAGGCCTTTTTAGCAACAATAATTGCATTTTTAATTAAAAAATATCTAAAAAATCTTGAATATATTTTTCCGATGCTTGCAGGGCTTGGTGCAGTAATTGGGCACGTCTTTCCAATTTATTTTAAATTTAAAGGTGGCAAAGGAGTAGCTTGCTTTTTTGGAATAATGCTTGCATATAATTTTCTATTATTTTTAATTTTTGTAGCAATATATGTAATACTAGTTTTTTCAACAAAATATGTTTCACTTGCTTCGGCAATAGGCTGTGGAATAATTCCTTATTTAGGTTTAATTTATATATTTTATGCAGGAACAATATTCGGCTATATGCAACAAAATGTCGTATATCCCGCACATGTAATTATTTTAATATTAGCTTCGCTCATAATAGTTGTTAAACATATACCCAATTTTAAACGAATATTCAAACATACAGAACCAAAAATTTTTAGCAAAACAAAAAAAGCATAA", "aa": "MAIIITKLRGNDIRKIGSQNPGATNALRNFGIKFAALVFAFDIFKAFLATIIAFLIKKYLKNLEYIFPMLAGLGAVIGHVFPIYFKFKGGKGVACFFGIMLAYNFLLFLIFVAIYVILVFSTKYVSLASAIGCGIIPYLGLIYIFYAGTIFGYMQQNVVYPAHVIILILASLIIVVKHIPNFKRIFKHTEPKIFSKTKKA", "aa_hexdigest": "b5fe4c87a59dca2d67aad069f6e626f6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8M5", "query_cov": 0.995, "subject_cov": 0.868995633187773, "identity": 0.98, "score": 366.0, "evalue": 2.57e-127, "valid": true, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "ec_ids": [ "2.3.1.15", "2.3.1.275" ], "uniref50_id": "UniRef50_D1J8M5", "cog_id": "COG0344", "cog_category": "I", "kegg_orthology_id": "K08591", "go_ids": [ "GO:0005886", "GO:0006654", "GO:0008374" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M5" ], "product": "Glycerol-3-phosphate acyltransferase" }, "genes": [ "plsY" ], "id": "AHCKFKLKGB_937", "locus": "GCA013367975_00936" }, { "type": "cds", "sequence": "contig_1", "start": 551043, "stop": 551420, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_036433001.1", "SO:0001217", "UniParc:UPI000512A6E5", "UniRef:UniRef100_A0A6A8Q407", "UniRef:UniRef50_D1J8M6", "UniRef:UniRef90_D1J8M6" ], "nt": "ATGGAATTATTAAAATATAGTTTAATTACAAAAAACGAATTCAATAATGAACCCTCTAAAACGATAGAAACAGAATTTGTTCCATATACCGAAGAAGTTGTTGATGAATTTATTCATATAAATTTTATTGATAATAATAATCAGGAATGTGATTTATTAGTCAATAGCGATACTTTAAAAATAAAATATTCTGGTTCATATATCGAGTTACAAAAAAACAAAAGAGTAAGCAATAAAATTCAAGAATTTTCTCTAGATTTTTATTTAAAAGATGTTTTTATTGAAGGAAACAAGATATCTGCTCATTATGATATTTTAGTTGGTAGTGATGTTATTGCAAGCAATATAATTAACATTGAAACAAAGACCGAAAAATAG", "aa": "MELLKYSLITKNEFNNEPSKTIETEFVPYTEEVVDEFIHINFIDNNNQECDLLVNSDTLKIKYSGSYIELQKNKRVSNKIQEFSLDFYLKDVFIEGNKISAHYDILVGSDVIASNIINIETKTEK", "aa_hexdigest": "30222493c3d8b15db772f468b12470ab", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000512A6E5", "ncbi_nrp_id": "WP_036433001.1", "uniref100_id": "UniRef100_A0A6A8Q407", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512A6E5", "RefSeq:WP_036433001.1", "UniRef:UniRef100_A0A6A8Q407" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8Q407", "uniref90_id": "UniRef90_D1J8M6" }, "psc": { "uniref90_id": "UniRef90_D1J8M6", "uniref50_id": "UniRef50_D1J8M6" }, "pscc": { "uniref50_id": "UniRef50_D1J8M6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14534.186899999986, "isoelectric_point": 4.650191307067872 }, "id": "AHCKFKLKGB_938", "locus": "GCA013367975_00937" }, { "type": "cds", "sequence": "contig_1", "start": 551401, "stop": 551517, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATTAGAAAAGCAACAACATTTGAGGAACATGGCCCTGAACTTGCAAAGGCAATCTATCTTTTTGGGGATGAATTAGTTTTTAAGAGATTAAATGGAATTATTAAAATATAG", "aa": "MPIRKATTFEEHGPELAKAIYLFGDELVFKRLNGIIKI", "aa_hexdigest": "6ad09b7c8d11245d00680763e5a808d9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4360.126999999999, "isoelectric_point": 8.16197147369385 }, "id": "AHCKFKLKGB_939", "locus": "GCA013367975_00938" }, { "type": "cds", "sequence": "contig_1", "start": 551941, "stop": 552057, "strand": "-", "frame": 1, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F", "UniRef:UniRef90_UPI003594285F" ], "nt": "ATGGAAGGAAAAAAGCTTTCAAAAAGAGATAAAACTTTAAAACAATTTATTGAAGACTATAAAAATGAAGGCTATCCTGCGGCAGCAATAATGAATTTTTTAGCCTTGTTAGGTTGA", "aa": "MEGKKLSKRDKTLKQFIEDYKNEGYPAAAIMNFLALLG", "aa_hexdigest": "57ad0b88b87cbb936a0cd5b0b7ea7e03", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 551893, "stop": 552150, "observations": { "mutation": [ 551943, 552099 ], "stop": [ 551943, 552099 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI003594285F", "query_cov": 1.0, "subject_cov": 0.4470588235294118, "identity": 0.8420000000000001, "score": 63.2, "evalue": 3.54e-12, "gene_start": 1, "gene_end": 38, "reference_start": 32, "reference_end": 69, "reference_sequence": "EHITNTPKQLAIYDAFGWETPRFGHLTIITNMEGKKLSKRDKFLKQFIEDYKNEGYRPDAIFNFLVLLGWTSADKSEVMSHQEII" }, "paralog": false, "identity": 0.8, "score": 136.0, "evalue": 1.23e-43, "description": "Internal stop codon at 551943, 552099. Nonsense mutation around 551943, 552099.", "psc": { "uniref90_id": "UniRef90_UPI003594285F", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI003594285F", "UniRef:UniRef50_UPI003594285F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F" ], "product": "glutamate--tRNA ligase family protein" } }, "id": "AHCKFKLKGB_940", "locus": "GCA013367975_00939" }, { "type": "cds", "sequence": "contig_1", "start": 552097, "stop": 552237, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00749.27" ], "nt": "ATGAAAAGCGATGGTTATCCAACATATAATTTTGCAGTTGTTGTTGATGACCACCAAATGGAAATTTCACATGTACTAAGAGGCGAAGAACACATCACAAACACACCAAAGCAATTAGCAATATACGATGCATTTGGATGA", "aa": "MKSDGYPTYNFAVVVDDHQMEISHVLRGEEHITNTPKQLAIYDAFG", "aa_hexdigest": "27ec22122b105c817e0888dc936fdbd6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00749.27", "name": "tRNA-synt_1c", "length": 45, "aa_cov": 0.9782608695652174, "hmm_cov": 1.0, "evalue": 1.2194397669780194e-20, "score": 68.9207763671875, "start": 2, "stop": 46 } ], "seq_stats": { "molecular_weight": 5238.7742, "isoelectric_point": 4.9293844223022445 }, "id": "AHCKFKLKGB_941", "locus": "GCA013367975_00940" }, { "type": "cds", "sequence": "contig_1", "start": 552607, "stop": 552798, "strand": "-", "frame": 1, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7", "UniRef:UniRef90_UPI002208A1AD" ], "nt": "ATGAAAACTATAAGAACAAGATATGCACCGAGCCCAACTGGGTATTTGCACATTGGTGGTGCAAGAACTGCATTATTTAACTATTTATTTGCAAAACATTTCAATGGTACTTTTGTTTTTAGGCTAGAAGATACTGACGTTGCTAGAAACGTTGTCGGAGGTGAAGAAAGTCAATTAAATAATTTAGCGTGA", "aa": "MKTIRTRYAPSPTGYLHIGGARTALFNYLFAKHFNGTFVFRLEDTDVARNVVGGEESQLNNLA", "aa_hexdigest": "025c80f5c6cbd3489fb6fa238db8d3c4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0016AA1FE4", "query_cov": 0.9841269841269841, "subject_cov": 0.8611111111111112, "identity": 0.613, "score": 81.6, "evalue": 3.58e-19, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A5J4P5N7" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 552433, "stop": 552789, "observations": { "mutation": [ 552609 ], "stop": [ 552609 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002208A1AD", "query_cov": 0.9523809523809523, "subject_cov": 0.4444444444444444, "identity": 0.9, "score": 114.0, "evalue": 2.38e-31, "gene_start": 4, "gene_end": 63, "reference_start": 3, "reference_end": 62, "reference_sequence": "MKIRTRYAPSPTGYLHIGGARTALFCYLYAKHFNGQFIFRLEDTDVKRNVPGGEESQLNNLAWLGIIPDESPLNPNPIYGKYRQSEKLDRYWKIANELIEKGFAYKAYDNSDELEQQKKKVMKKEYLVLGMINID" }, "paralog": false, "identity": 0.7711864406779662, "score": 184.0, "evalue": 2.38e-61, "description": "Internal stop codon at 552609. Nonsense mutation around 552609.", "psc": { "uniref90_id": "UniRef90_UPI002208A1AD", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002208A1AD", "UniRef:UniRef50_A0A654IDE7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7" ], "product": "Glutamate--tRNA ligase" } }, "id": "AHCKFKLKGB_942", "locus": "GCA013367975_00941" }, { "type": "cds", "sequence": "contig_1", "start": 553121, "stop": 553663, "strand": "-", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M8", "UniRef:UniRef90_D1J8M8" ], "nt": "ATGAAAAAACAAGCAATTATGACAAAATTTTTACTTTGTATAGGAAGTGTCGCTCCTATGTTTGCATTGCCACTAGTTGCGGCAAGTTGTAACAAAACAGCAACCATAACTCTCAATTCAAAAGCGAAAGAATCATTTGAAAAATATGGTTATCACGTTAAGGGTTCTGCACTTGATTTTAATAAAAATAATTATTCAGTAAGTAACCCGATATCGCCAAGAGATTCCTTGTACAAATTAACAAAAAATTTAAAAGATGAAAAAGGAAATCCAATTTACTTTAGAGATGCCGATGGAAATTTATTAAAATTTAAGGGTAAAAAATTGGAAAATGGTGAAATAGAATTCGATAGAGATGCTAATGGAAACTACATTTATGACAAAGAAAATGGATACCCAAGATTGAAAGAAGTATTGAATAATGAACTCGAAGTAATTGATGGAAAACCAGTGCAAAGAGTTTATAGAGAATTTTTTAGCAGATTTTTAGATTTTAATAATATTTCTCCAAATTACGATTTTAGAGTTTTCTCTTTTACATGA", "aa": "MKKQAIMTKFLLCIGSVAPMFALPLVAASCNKTATITLNSKAKESFEKYGYHVKGSALDFNKNNYSVSNPISPRDSLYKLTKNLKDEKGNPIYFRDADGNLLKFKGKKLENGEIEFDRDANGNYIYDKENGYPRLKEVLNNELEVIDGKPVQRVYREFFSRFLDFNNISPNYDFRVFSFT", "aa_hexdigest": "6504f2d4d59bae95327b42d8051a628f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 552818, "stop": 553663, "observations": { "mutation": [ 552859, 552904, 552994, 553027, 553072, 553123 ], "stop": [ 552859, 552904, 552994, 553027, 553072, 553123 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M8", "query_cov": 1.0, "subject_cov": 0.6360424028268551, "identity": 0.95, "score": 338.0, "evalue": 6.93e-116, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MKKQAIMTKFLLCIGSVAPMFALPLVAASCNKTATITLNSKAKESFEKYGYHVKGSALDFNKNNYSVSNPISPRDSLYKLTKNLKDEKGNPIYFRDANGNLLKFKGKKLENGEIEFDRDANGNYIYDKENGYPRLKEVLNNELEVIDGKPVQRVYREFFSRFLDFNNISPNYDFRVFSFTWEELINYFPATKNDSRYWPYRDNPRALFLVLYWTLKQNEAAPNWKSDFLDPSIESLLAKGKNRNVNTSIEEAPWPFYKGIMKGNKDAFWSNASEPITAIFEKP" }, "paralog": false, "identity": 0.9466192170818505, "score": 526.0, "evalue": 2.47e-192, "description": "Internal stop codon at 552859, 552904, 552994, 553027, 553072, 553123. Nonsense mutation around 552859, 552904, 552994, 553027, 553072, 553123.", "psc": { "uniref90_id": "UniRef90_D1J8M8", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J8M8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M8", "UniRef:UniRef50_D1J8M8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M8" ], "product": "Variable surface lipoprotein" } }, "id": "AHCKFKLKGB_943", "locus": "GCA013367975_00942" }, { "type": "cds", "sequence": "contig_1", "start": 553829, "stop": 554332, "strand": "+", "frame": 2, "gene": null, "product": "cysteine peptidase family C39 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E465ED2", "UniRef:UniRef90_UPI001E465ED2" ], "nt": "ATGATTTGCCACTATTTTAAATATAAGGCAGATATAAATTTTTTAAAGAAAAACACGATATACGACGAAGAAGGAGTGTCGCTATATTCATTTAAGAAAATTTCTGATTTTGTGGGGTTTGATACTAATATTTACAAAGTAGATTTTGTTAGTTTTATTTCAAATTTGAATGATGTTTCATTTCCGTTTGTCACAATATTGAAAAATGATAATTTCTTGCATTATACAATTGTTAAAAAAATTGATAAAGCCTTTGTTTATCTATGTGATTCTGAATTTTTGAATTATAGATTAGAAATTAATGAATTTAAAAATAAATTCATAAATGTTGTAGTTATATTAAGCAAAAAATTTAAAACCCCAAATTGCACAAAAATTAACTATAATCTATATCTAACACAAAACACAAAATTAACAATAATTAGTATATTTTTGGCAATTTTATTCAATGCTCTTATGCTTTCTTCTTCATTTTACATTAAATATGTTTTAAGCATTTTATGA", "aa": "MICHYFKYKADINFLKKNTIYDEEGVSLYSFKKISDFVGFDTNIYKVDFVSFISNLNDVSFPFVTILKNDNFLHYTIVKKIDKAFVYLCDSEFLNYRLEINEFKNKFINVVVILSKKFKTPNCTKINYNLYLTQNTKLTIISIFLAILFNALMLSSSFYIKYVLSIL", "aa_hexdigest": "c910d0a0e479542fc33879270d2f1493", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 553772, "stop": 554612, "observations": { "insertion": [ 554594 ], "mutation": [ 553775, 554330, 554381, 554408 ], "stop": [ 553775, 554330, 554381, 554408 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E465ED2", "query_cov": 1.0, "subject_cov": 0.5985663082437276, "identity": 0.97, "score": 316.0, "evalue": 3.39e-107, "gene_start": 1, "gene_end": 167, "reference_start": 20, "reference_end": 186, "reference_sequence": "MWINKQDSVKDCAIYTLQAMICHFFKYKADINFLKKNTIYDEEGVSLYSFKKISDFVGFDTNIYKVDFLSFISNLNDVSFPFVTILKNDNFLHYTIVKRIDKAFVYLCDSEFLNYRLEINEFKDKFINVVVILSKKFKTPNCTKINYNLYLTQNTKLTIISIFLAILFNALMLSSSFYIKYVLSVLWSENANNRLLSILIIFSWIAILKAITWLFKKIIINKLTFYYEKKLYLLVKERLKFASQKDLQKINQNDFMARILSISTITEYKAGLFLFYLKI" }, "paralog": false, "identity": 0.956989247311828, "score": 515.0, "evalue": 2.36e-188, "description": "Internal stop codon at 553775, 554330, 554381, 554408. Frameshift due to insertion around 554594. Nonsense mutation around 553775, 554330, 554381, 554408.", "psc": { "uniref90_id": "UniRef90_UPI001E465ED2", "product": "cysteine peptidase family C39 domain-containing protein", "uniref50_id": "UniRef50_UPI001E465ED2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E465ED2", "UniRef:UniRef50_UPI001E465ED2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E465ED2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E465ED2" ], "product": "cysteine peptidase family C39 domain-containing protein" } }, "id": "AHCKFKLKGB_944", "locus": "GCA013367975_00943" }, { "type": "cds", "sequence": "contig_1", "start": 554468, "stop": 554923, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTCAAAGAAAGATTAAAATTTGCAAGTCAAAAAGACCTGCAAAAAATTAACCAAAATGATTTTATGGCAAGAATATTAAGTATTTCAACTATTGCAGAATATAAAGCGGGTTTATTTTTGTTTTTATTTGAAAATATAATTATTTTTATTAGTTCAACATTGCTTTTAGGTCTGTTTAACTATAGAATATTATTAATTTGTTTATTAGGCATTTCAATAACCTTTGCAATCTCGTTATTCAGTTTAAAATATCAATCAAAAAAAGCAACAGAATTAATAAATTTAAAGATAAAATCAATTAAAATATTCAATAATTATTTTTTAAATTATGAATATAGGACGAACGATTTAAATAATTCAAATTCTAAGTTATTTCAATCGTTGTTTAACACTCAATATAATCTAGGAATATTCAATGAAACAAAAGATTCATTTATTACATTTATTTGA", "aa": "MLVKERLKFASQKDLQKINQNDFMARILSISTIAEYKAGLFLFLFENIIIFISSTLLLGLFNYRILLICLLGISITFAISLFSLKYQSKKATELINLKIKSIKIFNNYFLNYEYRTNDLNNSNSKLFQSLFNTQYNLGIFNETKDSFITFI", "aa_hexdigest": "0a630f70bc6b5430a08a7be35618cb39", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17647.52529999998, "isoelectric_point": 9.546881294250486 }, "id": "AHCKFKLKGB_945", "locus": "GCA013367975_00944" }, { "type": "cds", "sequence": "contig_1", "start": 555026, "stop": 555316, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTCCCCTATTGTAAATTTAGTATTCCTGATTTCAAATTCTTCAATAAATAAACAAGGAATAATAAGAATAAAATATTTATTAGAAATCACACAAAACAAGGATGGAAATATATCATTCAAACCCCAGAAGCAAAATAAAATAAACATATTAAATGCTTCAATAAATATTGGTTCAAAAAACGTTATAAACAATCTTAATTTAACAATTAAAAACAATCTAGAAATATCAGGGAAAAATGGAGTTGGTAAAAGTTTAATTTTGAATGCTATATTTAAAGTTTATTGA", "aa": "MNSPIVNLVFLISNSSINKQGIIRIKYLLEITQNKDGNISFKPQKQNKINILNASINIGSKNVINNLNLTIKNNLEISGKNGVGKSLILNAIFKVY", "aa_hexdigest": "90457469dcd71b1e3e23483789cdb969", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10638.34679999999, "isoelectric_point": 10.202009010314942 }, "id": "AHCKFKLKGB_946", "locus": "GCA013367975_00945" }, { "type": "cds", "sequence": "contig_1", "start": 555434, "stop": 555790, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTGCATATGTAAAAAAGACAATATAAAGGAAAATATTTTTCTTAAGAATTTCAAAAAATATAATTTAAATAACTTGTTTTTAGATATGAACTTACCTCTTAATAAATATATTGAAAATGGGGGAAATAATCTATCATCCGGCCAGAAGGAATTCATAATTATAATGCGACTTTTTTGCCAGAAATATTATTTTATTCTTTTGGATGAGGCTTTTGAAAACATTACAAATGAAATTTTTCTTAAATTAAAATTTTTAATTTTAAACTACCAAGATAGGGCTATTTTTATTGAAATTAGCCATAACAAAAAAATTGTTGCAAACAAGCGAAAAATTTTTGCTTTAAAAAAATAA", "aa": "MDCICKKDNIKENIFLKNFKKYNLNNLFLDMNLPLNKYIENGGNNLSSGQKEFIIIMRLFCQKYYFILLDEAFENITNEIFLKLKFLILNYQDRAIFIEISHNKKIVANKRKIFALKK", "aa_hexdigest": "ad3492312072d939909b2d012609c73b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14151.632599999977, "isoelectric_point": 9.444441032409667 }, "id": "AHCKFKLKGB_947", "locus": "GCA013367975_00946" }, { "type": "cds", "sequence": "contig_1", "start": 555849, "stop": 556301, "strand": "+", "frame": 3, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "GO:0004540", "GO:0042254", "GO:0046872", "RefSeq:WP_175933572.1", "SO:0001217", "UniParc:UPI00158CBF45", "UniRef:UniRef100_UPI00158CBF45", "UniRef:UniRef50_A0A448ZWQ4", "UniRef:UniRef90_D1J8N0" ], "nt": "ATGAATAGAATTAATTTTAAAAACCAAAGCTTTCATCGTTTTAATTTTATGAAAGATTTCTTAGATATTTTAGAAGAATCAAAAATAGAATTTGATGCAAAAAAATCACTTTGTGTTGATGTAATGTTTGTAACTGCAAAAAAGATGAAAAAGTTAAATAACTTACATAGACAAAAAAACTATACAACTGATATTTTAAGTTTCCCACTAGATAGTCAAGAAGAATTAAATTTTTTGGATGAAATTGAATTAGGACAAATTATTATTTCTCCGTGGAAAATAAAAAAACAAGCCAAGGAGTTCAATCATAGTTTAAGACGAGAATTTTGTTATATTTTTGCTCATGGAATTGCTCACCTATTTGGTTTTGATCACATTACTGAAGAAGAAGCAAAAATAATGAATGGGCATGTCGATAATATAATGAATAAATTAAACATAAAGAGAATATAA", "aa": "MNRINFKNQSFHRFNFMKDFLDILEESKIEFDAKKSLCVDVMFVTAKKMKKLNNLHRQKNYTTDILSFPLDSQEELNFLDEIELGQIIISPWKIKKQAKEFNHSLRREFCYIFAHGIAHLFGFDHITEEEAKIMNGHVDNIMNKLNIKRI", "aa_hexdigest": "767bf8c1f57b3dc52b92d6dfc834c15f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158CBF45", "ncbi_nrp_id": "WP_175933572.1", "uniref100_id": "UniRef100_UPI00158CBF45", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158CBF45", "RefSeq:WP_175933572.1", "UniRef:UniRef100_UPI00158CBF45" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158CBF45", "uniref90_id": "UniRef90_D1J8N0" }, "psc": { "uniref90_id": "UniRef90_D1J8N0", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "uniref50_id": "UniRef50_A0A448ZWQ4", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004540", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZWQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZWQ4" ], "product": "Endoribonuclease YbeY" }, "genes": [ "ybeY" ], "id": "AHCKFKLKGB_948", "locus": "GCA013367975_00947" }, { "type": "cds", "sequence": "contig_1", "start": 556315, "stop": 557160, "strand": "+", "frame": 1, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "GO:0003723", "GO:0005525", "SO:0001217", "UniRef:UniRef50_A0A501XB37", "UniRef:UniRef90_D1J8N1" ], "nt": "ATGAAAAAAGTTTGCAATGTATGCTTAATTGGGCGCCCAAATGTTGGAAAAAGCACGTTTTTAAATAATGTGTTAAATTTTAACCTATCAATAATTACGGATAAACCCCAAACAACAAAAGATAACATTAGAGGAATATACAATGATAAAGACTATCAAATCATTTTTATTGATACTCCAGGAATTCATAAAGCAGAAAATTTATTATCTGAAAGATTAAATAGTAAATCATATGAAGCTATTGAAAATTCTGATGTTGTTTTGTTTTTAACTCCCGCTAATGAAACAATCGGAACAGGAGATAAATTCATTATTGAAAAAATAAACGAAACTAATAATGAAAATAAAATTGCAGTTATTTCAAAAATTGATTTATTAAATACAAAAGAAGAAGCAGACAAAAAAGCAGAAGAATTAAAAAAACTTGGATTTGAAAAGGTTTTTGGCATTGGACAAAATTTGCCTACTTCTTATTCAGATTTGATCAATGAAATTAAAACCTATTGCTATGAAGATGAACCTTTGTACCCTGATGATCAAATTGGGGATATAACATTAAGATTTATGGCAAAAGAAATAATTAGAGAAACTGCTATAAACAATTTAAAATTTGAATTGCCACACTCAATTGCGGTTGAGATCGAACAATTTGACGACAAAAATGAAGATGAAGATTACAAGATCTATGCTTTAATTTATGTTAAAAAGAAGTCTCAGAAACCTATTTTAATCGGAAAAGACGGCCAAATGATTAAAAAAATCTCTATTGATTCACGACATAAAATGGAAAAAGTTTTTGATCACAAAATCTTTTTAAATATTAAAGTAAAAATCAGCGAAGATTGA", "aa": "MKKVCNVCLIGRPNVGKSTFLNNVLNFNLSIITDKPQTTKDNIRGIYNDKDYQIIFIDTPGIHKAENLLSERLNSKSYEAIENSDVVLFLTPANETIGTGDKFIIEKINETNNENKIAVISKIDLLNTKEEADKKAEELKKLGFEKVFGIGQNLPTSYSDLINEIKTYCYEDEPLYPDDQIGDITLRFMAKEIIRETAINNLKFELPHSIAVEIEQFDDKNEDEDYKIYALIYVKKKSQKPILIGKDGQMIKKISIDSRHKMEKVFDHKIFLNIKVKISED", "aa_hexdigest": "9056aa010ce493b61fcf1ad34ca80ec2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8N1", "query_cov": 1.0, "subject_cov": 0.95578231292517, "identity": 0.982, "score": 536.0, "evalue": 4.4e-192, "valid": true, "gene": "era", "product": "GTPase Era", "uniref50_id": "UniRef50_A0A501XB37", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501XB37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501XB37" ], "product": "GTPase Era" }, "genes": [ "era" ], "id": "AHCKFKLKGB_949", "locus": "GCA013367975_00948" }, { "type": "cds", "sequence": "contig_1", "start": 557201, "stop": 557665, "strand": "+", "frame": 2, "gene": null, "product": "Hypothetical lipoprotein (MG045 family)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9E7F", "UniRef:UniRef90_UPI00215D9E7F" ], "nt": "GTGAAACGATTAACTACACATAGAACGAGAGTTATTTTTATAGCAATAATTGCCTTACTAACACTTGGATTATTTTTAATAATTTTATTCTTTAAAATTAGCTCCCCATTTAAAGTAAGTATATATAACTACGAATCATACTTAGATAAGAAAATAATTACCAAATTAAAACAAAATTATTCATATCACGCCTTTACAAATCTAGATGAGTTTACTAGAGCAATAAATAACGAAAAAGCCGTTGCTGGAGTTGGAAGTGATTATCAAATTGCCCAACTAATTCTTGAAAAAAAATTAAGAAAAATTAACTTTGCAAAATTGTTTAATGATTCAAAATTTAATGATTCAACCCAAATAAAGAAGCTTTATCCAGAAATTGTTAATAAACATTTTGATTTATTTGAAAAATTTATCATTAAAAAAATAAGACAAATAAATCCTAAAAACATCAAGCAAAATGATTGA", "aa": "MKRLTTHRTRVIFIAIIALLTLGLFLIILFFKISSPFKVSIYNYESYLDKKIITKLKQNYSYHAFTNLDEFTRAINNEKAVAGVGSDYQIAQLILEKKLRKINFAKLFNDSKFNDSTQIKKLYPEIVNKHFDLFEKFIIKKIRQINPKNIKQND", "aa_hexdigest": "fb70c19afa402ecf107f455f26e3bd56", "start_type": "GTG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 557201, "stop": 557968, "observations": { "mutation": [ 557663, 557903, 557957 ], "stop": [ 557663, 557903, 557957 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D9E7F", "query_cov": 1.0, "subject_cov": 0.4031413612565445, "identity": 0.961, "score": 279.0, "evalue": 2.1e-91, "gene_start": 1, "gene_end": 154, "reference_start": 1, "reference_end": 154, "reference_sequence": "MKRLTTHRTRVIFIAIIALLTLGLFLIILFFKISSPFKVSIYNYESYLDKKIITKLKQNYSYHAFTNLDEFTRAINNEKAVAGVGSDYQIAQLILEKKLRKINFAKLFNNSKFNDSTQIKNLYPEIVNKHFDLFEKFIIKKIRQINPENFQQTDWQNISHTKFLPFIYYDKNNNILGFEIDQKKGIDHFYEFLIPYFIMDKMVVYNTDKSPNKFTERSNLKNNENFNNLESLSSWKDIIKTLVSKYKNPRVYWTNWFQDNAMIGQFYGYESLNKLKYFSKQNEWADINHENFKEIINDWVSLVKDATGESIKNSKTNKLATDGQELVSSIIEPINGKADVSIMYNGDALDAYYGEDNFKKLGNIPHIGFKRPKILIWILMLE" }, "paralog": false, "identity": 0.9254901960784314, "score": 473.0, "evalue": 2.25e-170, "description": "Internal stop codon at 557663, 557903, 557957. Nonsense mutation around 557663, 557903, 557957.", "psc": { "uniref90_id": "UniRef90_UPI00215D9E7F", "product": "Hypothetical lipoprotein (MG045 family)", "uniref50_id": "UniRef50_UPI00215D9E7F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D9E7F", "UniRef:UniRef50_UPI00215D9E7F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9E7F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9E7F" ] } }, "id": "AHCKFKLKGB_950", "locus": "GCA013367975_00949" }, { "type": "cds", "sequence": "contig_1", "start": 557726, "stop": 557905, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTTTTGAAATTGACCAAAACCAAGGAGTTGACCACTTTTACGAATTTTTAATCCCTTATTTTATAATGGATAAAATGGTTGTTTATAATACAGATAAAAGTCCAAATAAATTTACTGAAAGAAGCAATTTAAAATCTAATGAAAACTTTAACGATTTAGAAAATTTAAATTCTTGA", "aa": "MGFEIDQNQGVDHFYEFLIPYFIMDKMVVYNTDKSPNKFTERSNLKSNENFNDLENLNS", "aa_hexdigest": "79ecff35a4edeac384d472753c7ddc08", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7042.714900000001, "isoelectric_point": 4.450743293762207 }, "id": "AHCKFKLKGB_951", "locus": "GCA013367975_00950" }, { "type": "cds", "sequence": "contig_1", "start": 557984, "stop": 558346, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGTCAATTTTATGGTTATGAATCTCTTAACAAATTAAAATATTTTTCTAAGCAAAACGAATGGGCAGATATAAATCAAGAAAATTTTAAAGAGATTATTAATGATTGGGTTTCTTTGGTTAAGGATGCAACAGGAGAAAGTATAAAAAATTCTAAAACAAATAAATTAGCCACTGATGGACAAGAATTAGTTAGTTCAATAATAGAGCCAATTAATGGCAAGGCAGATGTTTCAATTATGTATAATGGAGATGCCTTAGATGCATATTATGGGGAAGACAATTTTAAAAAATTAGGTAATATTCCGCATATTGGTTTTAAAAGACCAAAAAATACTTACATGAATATTGATGCTTGA", "aa": "MIGQFYGYESLNKLKYFSKQNEWADINQENFKEIINDWVSLVKDATGESIKNSKTNKLATDGQELVSSIIEPINGKADVSIMYNGDALDAYYGEDNFKKLGNIPHIGFKRPKNTYMNIDA", "aa_hexdigest": "65752660133932a01f171f486ca62d54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13636.123599999983, "isoelectric_point": 5.1215002059936525 }, "id": "AHCKFKLKGB_952", "locus": "GCA013367975_00951" }, { "type": "cds", "sequence": "contig_1", "start": 558467, "stop": 558865, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAATTTAATATCAAAAGACAACAAATTAAAAAAAGATGAAATTATTAATGAGCTATTTACAGACAAAAACATGTCTATGCCCAAAAAAATTAAAGACATTAAAAAAGATTTTTTTGCAGAATATTTTGACTATTTCAGAGAAGCATTTGAAGCAATGAATTTACCCATTGTAAACAATTTTCATTCAATAAATTACACACCAGGCTTTAAAAATGTAAAAAATTTTATTGAAAAATGGTACTTCTTGAATAAAAATCAGGAAGTTGATAAAACTGCTTTAGAGATATTTAATCCCGAATTTAATAAAAAAATCAATCACAGAATTTACCAACCACTTGATTTAAATTTAAAAACAAAAATAATAGATTATTACTTTCAAAAAACAAAATCATAA", "aa": "MLNLISKDNKLKKDEIINELFTDKNMSMPKKIKDIKKDFFAEYFDYFREAFEAMNLPIVNNFHSINYTPGFKNVKNFIEKWYFLNKNQEVDKTALEIFNPEFNKKINHRIYQPLDLNLKTKIIDYYFQKTKS", "aa_hexdigest": "7e5a46093c61ce708272a332774e1ecf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16051.376499999988, "isoelectric_point": 9.222347450256347 }, "id": "AHCKFKLKGB_953", "locus": "GCA013367975_00952" }, { "type": "cds", "sequence": "contig_1", "start": 558894, "stop": 559091, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00112F2DA6" ], "nt": "ATGGAAAATAAAATTAAAACACCAAACAAAAATGAAGAACCAATAATCCAATTAGTAGACGTTGTTAAAGAATTTGAAGATAAAACAGTTTTAGATAATGTTAACTTGTCAATAAATAAAGGTGAATTTATAACATTATTAGGTCCTTCGGGTTCAGGAAAAACAACTATATTGAGAATTATTGGTGGTTTTGAATGA", "aa": "MENKIKTPNKNEEPIIQLVDVVKEFEDKTVLDNVNLSINKGEFITLLGPSGSGKTTILRIIGGFE", "aa_hexdigest": "7f9b71fd612db45245843a321d9ed6c6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI001E511DD0", "query_cov": 0.9538461538461539, "subject_cov": 0.8493150684931506, "identity": 0.532, "score": 73.6, "evalue": 6.32e-16, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI00112F2DA6" }, "pscc": { "uniref50_id": "UniRef50_UPI00112F2DA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00112F2DA6" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_954", "locus": "GCA013367975_00953" }, { "type": "cds", "sequence": "contig_1", "start": 559524, "stop": 559883, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "nt": "ATGAACCAAGAAGTATCGGAAATCATGGATCTTGTTGGTTTGAAGGGAAATGAAAAGAAAAATGTTAATTTCCTATCTGGTGGTATGAAACAACGTGTTGCACTTGCTCGCTCGCTTGTTATTGAACCTGAAATTCTTCTACTAGATGAACCATTAAGTGCTTTGGATGCTAAAATTAGGCAAAAAATGCAAACATTATTAAGGGATATTCAAAAAGAATTAAAAATTACGTTCATTTTTGTTACTCACGATAGAAATGAAGCATTGCAATTAAGTGATAGAATCGCCATTATTAGAGATGGTAAGGTTGAACAATTTGCTTCGCCAAAAGATTTATATGATTATCCAGTAAATAAATGA", "aa": "MNQEVSEIMDLVGLKGNEKKNVNFLSGGMKQRVALARSLVIEPEILLLDEPLSALDAKIRQKMQTLLRDIQKELKITFIFVTHDRNEALQLSDRIAIIRDGKVEQFASPKDLYDYPVNK", "aa_hexdigest": "954e1337d5f672b866653dc8a03c10ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00237D6FE8", "query_cov": 0.9747899159663865, "subject_cov": 0.9133858267716536, "identity": 0.517, "score": 122.0, "evalue": 1.15e-33, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00237D6FE8" }, "pscc": { "uniref50_id": "UniRef50_UPI00237D6FE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "AHCKFKLKGB_955", "locus": "GCA013367975_00954" }, { "type": "cds", "sequence": "contig_1", "start": 559953, "stop": 560195, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAAAATTTTTGATACTATTCACAAAGAATTTGAACCAGGAGAAAACCTTGATGCATTAATTAGACCCGAAGATGTTTATGTTACCAAAGTAAAAGCTAAATCCAAATTTATTGGCAAAATAATTAAATCAACATATAGTGGTAGTTACTACAATTATGAGGTTTTAGTAAAAAATAAAATAATAAAAATGGAAACATCAATAAACTATGAAGTGGGTAAGGAAGTATATTTAGATTGA", "aa": "MGKIFDTIHKEFEPGENLDALIRPEDVYVTKVKAKSKFIGKIIKSTYSGSYYNYEVLVKNKIIKMETSINYEVGKEVYLD", "aa_hexdigest": "a4241d06e7d04c26a50daf9835bcc07c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9271.616300000002, "isoelectric_point": 7.863031578063965 }, "id": "AHCKFKLKGB_956", "locus": "GCA013367975_00955" }, { "type": "cds", "sequence": "contig_1", "start": 560236, "stop": 560517, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAGGAACTGGCAGAACAAAACGAGATTAACAATGGAAAAACAACTGAAATCAACTTCTTATAAAGAAATAGACAAAAAAACTAAAAAAAGTAGTAAATTTGCAAAATTCTTTTTGGGTGCAAACAAATTACAACTTTCTTTGCTAATTCCTTATCTAATATTTTCAATTTTATTTATTATTTTGCCATTAATTTTGATATTTATTTATGCATTCAAACCACTAGAAGGACAAGGTCATCTTGCAAACTGGGAAATAGCAGGTAAATCATCAACTTGA", "aa": "MPRNWQNKTRLTMEKQLKSTSYKEIDKKTKKSSKFAKFFLGANKLQLSLLIPYLIFSILFIILPLILIFIYAFKPLEGQGHLANWEIAGKSST", "aa_hexdigest": "1289d5cd50e81ec7724349004f04a562", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10778.760299999987, "isoelectric_point": 10.106466865539549 }, "id": "AHCKFKLKGB_957", "locus": "GCA013367975_00956" }, { "type": "cds", "sequence": "contig_1", "start": 560518, "stop": 561171, "strand": "+", "frame": 1, "gene": "potB", "product": "Spermidine/putrescine ABC transporter permeaseprotein potB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8N4" ], "nt": "ATGATTATTCTTAGATCAATCGGAACCGGAATATGTGCAGCATTTTTTGCATTAATTATTGGTTTTCCATATGCCTATATTATTGCAAGAAGCAAATCAATGATATTTAGAACTTTAGGATTAACATTGATTCTAAGCCCGCTTGCAATATTCACAATTTCAAAAGCTTTAGCAGTTAGAGGTTTATTTTCTGCTATATTTGACGAAAATGAATTAAATAATAATTTCTTTCTAATCTTTGGAATGGTGTATTTGTATATCCCTTTTATGGTAATGCCATTATATCAAGTTATTAAGGATATGCCTAACAATATAATTGAAGCATCTCAAGATTTGGGATATTCAAAAACGCAAACTGTTTTTAAAGTTATATTACCCTACTGTATGAAGGCTATATTGAGTGGTTTTGGAGTTGTTCTTATGATGTCAGCGACATCAATAATTCTTTCAGATAAATTGTTGCCAAATGGTAGTCAAAAGCAACTTATAGGAAATCTTATAAATCAGTTTGCTAATACAGCAAACCCTTACGACTTAGCAAATGCCTCTACATTAGTTATTATCACTTTGGCTGTTTTATTAACAATATATGGAATTATTTATGGAATACCTTATTTAATTTCGAAAAGAAAGCAAGGAGGAATATATGAATAA", "aa": "MIILRSIGTGICAAFFALIIGFPYAYIIARSKSMIFRTLGLTLILSPLAIFTISKALAVRGLFSAIFDENELNNNFFLIFGMVYLYIPFMVMPLYQVIKDMPNNIIEASQDLGYSKTQTVFKVILPYCMKAILSGFGVVLMMSATSIILSDKLLPNGSQKQLIGNLINQFANTANPYDLANASTLVIITLAVLLTIYGIIYGIPYLISKRKQGGIYE", "aa_hexdigest": "e85be2a79d3e66b5ba532116f4c918c5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00352DB7BA", "query_cov": 1.0, "subject_cov": 0.8037037037037037, "identity": 0.843, "score": 357.0, "evalue": 1.38e-122, "valid": false, "product": "ABC transporter permease", "uniref50_id": "UniRef50_D1J8N4" }, "pscc": { "uniref50_id": "UniRef50_D1J8N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8N4" ], "product": "Spermidine/putrescine ABC transporter permeaseprotein potB" }, "genes": [ "potB" ], "id": "AHCKFKLKGB_958", "locus": "GCA013367975_00957" }, { "type": "cds", "sequence": "contig_1", "start": 561164, "stop": 561301, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTAAAAGATTTTTGAAGTATTCTTATGTATTAATAATTCTTGCTTGTTTATATATTCCAATCATTTTTGGAACAATATATAGTTTTAATGCACCTTCGGATAAGGGAATATTTTCTGTAACCTCATGA", "aa": "MNKFKRFLKYSYVLIILACLYIPIIFGTIYSFNAPSDKGIFSVTS", "aa_hexdigest": "6646166da8bd21584e28127e416b7b56", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5184.162200000001, "isoelectric_point": 9.580856132507321 }, "id": "AHCKFKLKGB_959", "locus": "GCA013367975_00958" }, { "type": "cds", "sequence": "contig_1", "start": 561470, "stop": 561721, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATCAACATCAAATATTCCTTTAATTAATCCAGATGTTATTACCGGTTTAACACTAGCAATTGTTTTAAATCTTATATTTTTTACCACACTAAAAGCAACCAACGAAGGATTTTTTAGAGCAATAATTTCACATACAGTAATGACTCTTCCTTATGGAATATTAATTATGTTGCCAAAAAGTGATAAATTTTCAAAAAATATCTTTGAAGCTAGCCAAGATCTAGGTTATTCAAAATTTAAAACATGA", "aa": "MQSTSNIPLINPDVITGLTLAIVLNLIFFTTLKATNEGFFRAIISHTVMTLPYGILIMLPKSDKFSKNIFEASQDLGYSKFKT", "aa_hexdigest": "c816bf02c6619802a6f11e59c2631485", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9241.785499999993, "isoelectric_point": 9.157943534851075 }, "id": "AHCKFKLKGB_960", "locus": "GCA013367975_00959" }, { "type": "cds", "sequence": "contig_1", "start": 561743, "stop": 561874, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTTCAATAGGGTTTACATTTGTAATCACAATGACGCTTTCTTTTGATGACTTCATAATTACTAGAATTGTTTCAAATACAGAAACCTTGGGTACTCAATTATATGAAGGTCAATTTCAAGCATGA", "aa": "MLGSIGFTFVITMTLSFDDFIITRIVSNTETLGTQLYEGQFQA", "aa_hexdigest": "2f3fb28131a84edda84bf5710dc59e95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4807.450900000001, "isoelectric_point": 4.0500284194946286 }, "id": "AHCKFKLKGB_961", "locus": "GCA013367975_00960" }, { "type": "cds", "sequence": "contig_1", "start": 562025, "stop": 562360, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGATCACGAATTTTAAAAGCTATTAGTTTAATATTTTTACCCTTGATTTTTATTGCTTTATTCCTTGCTTTTTTGGTTATAAAATCAACTAATCAATATAGACCTAGCATCTATAACTATGAATCTTATTTAGCTCCAACCATTATTAACAAATTAAAATCAAAATACAATTTCAAAGAATTTAAAGAAGTTAGTGAGTTTACGCAAGCATTATTGCAAGATAAAGCTATTGCTGGAATTGGAAGCGACTTTCAAGCTGCTCAACTTATTTTAGATAGGAAGATTCAAAAAATAGATTTTAGTCTAGTTTATGGTGAAAAATACAAAGATTGA", "aa": "MRSRILKAISLIFLPLIFIALFLAFLVIKSTNQYRPSIYNYESYLAPTIINKLKSKYNFKEFKEVSEFTQALLQDKAIAGIGSDFQAAQLILDRKIQKIDFSLVYGEKYKD", "aa_hexdigest": "81f3f97ecaacd584c7ca9f8b5e60cd7e", "start_type": "TTG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12880.004699999987, "isoelectric_point": 9.551523017883298 }, "id": "AHCKFKLKGB_962", "locus": "GCA013367975_00961" }, { "type": "cds", "sequence": "contig_1", "start": 563009, "stop": 563125, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACAATGGTGATGCATTGGATGCATTCTATTCTGAAGATAATTTTCAATCGGTTGAAAGTGGTTTAATAAGGTTTATTAGACCTAAAAACACATATATGCTAATGGATAATTGA", "aa": "MYNGDALDAFYSEDNFQSVESGLIRFIRPKNTYMLMDN", "aa_hexdigest": "20c0efe0d8ec23b32898ec4eb584397d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.9336, "isoelectric_point": 4.232879447937012 }, "id": "AHCKFKLKGB_963", "locus": "GCA013367975_00962" }, { "type": "cds", "sequence": "contig_1", "start": 563264, "stop": 563416, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAACAATTAAAACAATCTGCTGAAGCTTCTTTTTTTGAAACCATACATAATATTGACAAAACTTTAAAAAATAAGTTAATTCAAGAATTTCTAAAAAATAAAGAATTTTTCAATATAAACGAAAAATCAACTAGCTTTGAAGAATGA", "aa": "MTKQLKQSAEASFFETIHNIDKTLKNKLIQEFLKNKEFFNINEKSTSFEE", "aa_hexdigest": "c2205a1656575510b7deb76e03c503f0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5968.699199999997, "isoelectric_point": 6.571235466003418 }, "id": "AHCKFKLKGB_964", "locus": "GCA013367975_00963" }, { "type": "cds", "sequence": "contig_1", "start": 563537, "stop": 563641, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGATGCTTTTGCAAATAGTGAAATAGGAAGCATATCTAATTTTGATTATGTATCATATACTCCAACTGAAAAAATAATTTATGACTTTATTAAAAAATGA", "aa": "MSDAFANSEIGSISNFDYVSYTPTEKIIYDFIKK", "aa_hexdigest": "6a71f98844c169c361765855b19144da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3895.3002000000015, "isoelectric_point": 4.436874580383301 }, "id": "AHCKFKLKGB_965", "locus": "GCA013367975_00964" }, { "type": "cds", "sequence": "contig_1", "start": 564025, "stop": 564570, "strand": "+", "frame": 1, "gene": "asnS", "product": "asparagine--tRNA ligase", "db_xrefs": [ "COG:COG0017", "COG:J", "EC:6.1.1.22", "GO:0004816", "GO:0005524", "GO:0006421", "KEGG:K01893", "SO:0001217", "UniRef:UniRef50_Q4A9S1", "UniRef:UniRef90_D1J8N7" ], "nt": "ATGCAATTAGTTATTAAGGGAGATCTAGCATCAAAGAAAGAAATTTCTGAAATTGCCATGGGCTCGGCAGTTAGTGTAATAGGTAAATTACATTTAACGCCAGAAGCAATGCAACCATTTGAACTAAGTGTCTCAAAATTTTTATACATAAACAATCCACAAGAAGATTTTCCAATCCAAAAGAAAGAAACATCTCTTGATTTTTTAAGAGAAATTCCTCAATTAAGACATAGAACAAGTTTATTTAGAGCAATTATGTTAATCAGAAACTCTCTTATGTTTGAAATTCATAAATATTTCCAATCTAAAGGCTTTTTGAATCTTTCATCTCCAATAATCACATCAAACGATGGTGAAGGAGCAGGAGAAACTCTGCTTGTTGACGATGAAAGCAAAGATTATTTTTTCAAACAAAAAGCATTTTTAGGTGTAACTGGACAATTGCACGCCGAATCATATGCTGAAGGATTTAAAAAAGTTTATACATTCGGACCAACATTTAGAGCTGAAAATTCTCACACTTCGCGTCATCTAGCAGAATTTTGA", "aa": "MQLVIKGDLASKKEISEIAMGSAVSVIGKLHLTPEAMQPFELSVSKFLYINNPQEDFPIQKKETSLDFLREIPQLRHRTSLFRAIMLIRNSLMFEIHKYFQSKGFLNLSSPIITSNDGEGAGETLLVDDESKDYFFKQKAFLGVTGQLHAESYAEGFKKVYTFGPTFRAENSHTSRHLAEF", "aa_hexdigest": "2fd561fcbfa60092102737c35fa34b23", "start_type": "ATG", "rbs_motif": null, "genes": [ "asnS" ], "truncated": "both", "pseudogene": { "start": 563884, "stop": 564873, "observations": { "mutation": [ 563953, 564568 ], "stop": [ 563953, 564568 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8N7", "query_cov": 1.0, "subject_cov": 0.40401785714285715, "identity": 0.9890000000000001, "score": 355.0, "evalue": 7.05e-120, "gene_start": 1, "gene_end": 181, "reference_start": 48, "reference_end": 228, "reference_sequence": "MNIKQLYKQISQLQENVELTIEGWIISNRGNDKIRFITINDGSTIANMQLVVKGELASKKEISEIAMGSAVSVIGKLHLTPEAMQPFELSVSKFLYINNPQEDFPIQKKETSLDFLREIPQLRHRTSLFRAIMLIRNSLMFEIHKYFQSKGFLNLSSPIITSNDGEGAGETLLVDDESKDYFFKQKAFLGVTGQLHAESYAEGFKKVYTFGPTFRAENSHTSRHLAEFWMMEPEVAFCDLKGIIEITDDMLKNVIANTLKNYPEEMKFLDSLQNNKLIESLNNFINKKLTIIEYKDVVENLKNYVDKFEEKDIFFGMDLASEHEKFLAENIVKGPVAVINYPKDIKAFYMYQNDDGKTVAAFDLLVPGIGELVGGSQRENRYQKLVDRINELKITQETLQWYLDLRRFGYAPSSGFGLGFERLVMYVTGTSNIRDVIPFPRVAGSIKM" }, "paralog": false, "identity": 0.9848024316109423, "score": 637.0, "evalue": 1.56e-233, "description": "Internal stop codon at 563953, 564568. Nonsense mutation around 563953, 564568.", "psc": { "uniref90_id": "UniRef90_D1J8N7", "gene": "asnS", "product": "asparagine--tRNA ligase", "ec_ids": [ "6.1.1.22" ], "uniref50_id": "UniRef50_Q4A9S1", "cog_id": "COG0017", "cog_category": "J", "kegg_orthology_id": "K01893", "go_ids": [ "GO:0004816", "GO:0005524", "GO:0006421" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8N7", "EC:6.1.1.22", "UniRef:UniRef50_Q4A9S1", "COG:COG0017", "COG:J", "KEGG:K01893", "GO:0004816", "GO:0005524", "GO:0006421" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A9S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A9S1" ], "product": "Asparagine--tRNA ligase" } }, "id": "AHCKFKLKGB_966", "locus": "GCA013367975_00965" }, { "type": "cds", "sequence": "contig_1", "start": 564571, "stop": 565086, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATGGAACCAGAAGTAGCCTTTTGTGATTTAAAAGGTATCATAGAAATTACAGACGATATGTTAAAGAATGTTATTGCAAATACATTAAAAAACTATCCCGAAGAAATGAAATATTTGGATTCATTACAAAACAATAAATTAATTGAATCATTAAACAACTTTATCAATAAGAAATTAACAATAATTGAATATAAAGACGTTGTTGAAAATCTTAAGAACTATGTAGATAAATTCGAAGAAAAAGATATCTTTTTTGGAATGGACTTAGCTTCAGAACACGAAAAATTCCTTGCCGAAAACATCGTAAAAGGCCCTGTTGCAGTTATTAATTATCCAAAAGACATAAAGGCTTTTTATATGTATCAAAATGATGATGGCAAAACAGTCGCTGCCTTTGACTTACTAGTACCAGGAATTGGCGAATTAGTAGGGGGAAGTCAACGTGAAAATAGATATCAAAAACTTGTTGATAGAATCAATGAACTAAAAATCACTCAAGAAACATTACAATGA", "aa": "MMEPEVAFCDLKGIIEITDDMLKNVIANTLKNYPEEMKYLDSLQNNKLIESLNNFINKKLTIIEYKDVVENLKNYVDKFEEKDIFFGMDLASEHEKFLAENIVKGPVAVINYPKDIKAFYMYQNDDGKTVAAFDLLVPGIGELVGGSQRENRYQKLVDRINELKITQETLQ", "aa_hexdigest": "8f0ca0125e857cf9caa73d71b2fa3258", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 177, "aa_cov": 0.9824561403508771, "hmm_cov": 0.807909604519774, "evalue": 3.3516303874701395e-21, "score": 70.90122985839844, "start": 1, "stop": 168 } ], "seq_stats": { "molecular_weight": 19751.453799999996, "isoelectric_point": 4.737552833557129 }, "id": "AHCKFKLKGB_967", "locus": "GCA013367975_00966" }, { "type": "cds", "sequence": "contig_1", "start": 565090, "stop": 565230, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "TTGGACTTAAGAAGATTTGGTTACGCCCCAAGTAGTGGTTTTGGTTTAGGCTTTGAAAGACTTGTAATGTATGTTACTGGCACAAGTAACATAAGAGATGTTATTCCTTTTCCTCGTGTTGCCGGCTCAATTAAAATGTAG", "aa": "MDLRRFGYAPSSGFGLGFERLVMYVTGTSNIRDVIPFPRVAGSIKM", "aa_hexdigest": "c5f8245265dc4414fa8caea3ce9719e4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 39, "aa_cov": 0.8478260869565217, "hmm_cov": 1.0, "evalue": 1.6511814128905356e-13, "score": 45.63631820678711, "start": 2, "stop": 40 } ], "seq_stats": { "molecular_weight": 5110.932900000001, "isoelectric_point": 10.24610538482666 }, "id": "AHCKFKLKGB_968", "locus": "GCA013367975_00967" }, { "type": "cds", "sequence": "contig_1", "start": 565237, "stop": 565626, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAATAATTTAACAATATTAATACCTATATATCATCCAACTCTAACAATTAATGAGATATTAAAAAATTTATATAAACAAAAACAACAAAATTTTAATTTAGTAATCGCTATAGATAAGCCTTCTGAATCAGAACTTTATGAATTAGAAAAAGCAAAAACCTTTTTTTCAAATAGACTTCAAATAATAATCAATACAAATCACCAACACATTGATTCAATAATTAGTAGTTGCTTAAACATTACAAAAACAAAATACACATTTATTATGTATTCATATATTGAAATTGAGTATGATTTTATTTCGAAAATAAATAAACTTTTATTAGAATTTAAAGATGTTGATATTATAAGTTTTGACGCATATACAAAGGGTATATCTTGA", "aa": "MQENNLTILIPIYHPTLTINEILKNLYKQKQQNFNLVIAIDKPSESELYELEKAKTFFSNRLQIIINTNHQHIDSIISSCLNITKTKYTFIMYSYIEIEYDFISKINKLLLEFKDVDIISFDAYTKGIS", "aa_hexdigest": "832ac88b1f4755a0e7b3b13d06f85896", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15202.394499999984, "isoelectric_point": 5.884904670715332 }, "id": "AHCKFKLKGB_969", "locus": "GCA013367975_00968" }, { "type": "cds", "sequence": "contig_1", "start": 565627, "stop": 565869, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTCAACAAATATCCTAAACAAAATAAAGAAATAAATATTCATATTGAAGAAGAAAATGTTGCCAAAATAATTCCTTTTATGTACAATTTTATTGCAAAAACTTCTTTACTTATTGAAGCAACAAATCAACACAATAATAAACACCTTAGCGAGCAATATTCACCCAATATACTTTTTAAGACTTTAATTAATGCAAACAAAATTCTTTTAACAAAAGATATTCAAGTAATTGATTGA", "aa": "MNFNKYPKQNKEINIHIEEENVAKIIPFMYNFIAKTSLLIEATNQHNNKHLSEQYSPNILFKTLINANKILLTKDIQVID", "aa_hexdigest": "ded7b5cbc4f638400798f88af8ddfa61", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9399.75859999999, "isoelectric_point": 8.00518436431885 }, "id": "AHCKFKLKGB_970", "locus": "GCA013367975_00969" }, { "type": "cds", "sequence": "contig_1", "start": 566565, "stop": 566792, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATTTTTGCTTTATTTATGATTTTTATATCTCCTCAAACTTTTGAACAAGCAGCAAATCTTTCTCAAACAAAACCTTTAGACCAACTTCAAAAAGCTTTTCTTTCAATTCCTAGTGTGCTTTCAGCATTAGCCACATTTTTAGAATTCTTATTTATTTTAAGCAATGCTAAGGGAGATAAAATTGGTTTAAATGATAAATTTTCCGGATCCTATACAGTTTGA", "aa": "MIIFALFMIFISPQTFEQAANLSQTKPLDQLQKAFLSIPSVLSALATFLEFLFILSNAKGDKIGLNDKFSGSYTV", "aa_hexdigest": "df986b4c6b60e77e807cae32d1354ee6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8291.627799999993, "isoelectric_point": 6.000344657897949 }, "id": "AHCKFKLKGB_971", "locus": "GCA013367975_00970" }, { "type": "cds", "sequence": "contig_1", "start": 567017, "stop": 567697, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF00580.27" ], "nt": "ATGATTGATTTAAAAGATTTAAATGAACAGCAAAAAAGTGCCGTTATATATAACGAAGGACCTTTAAGAATTATTGCTGGCGCTGGTTCAGGAAAAACTAGAGTTTTAACATATAAGATTGCTTATTTAATCCAAAAATTAGGTGTTCTACCTAATCGTATTTTGGCACTAACTTTTTCAAATAAGGCTGCAAACGAAATGAAACAAAGGGTATTTAATCTACTTGCAGAAGATAACCCTAGCAATCTTTCTCCCTATATTTCAACATTCCATGCAATGTGTGCAAAAATATTGAGAAAAGAAATATATAACTTTGGATATGAAAACGATTTTCAAATTCTTGATGAATTAGACCAAAAAGAAATATTAAAAATTGTTTACTCAGAACTTGATATTTCACCAACAGAATTTACATTCTCTTCAATAATTTCATTTATTCAAAACAAAAAGAATTCAATCGAATATTTAAACAACTCAATTAGCGATAGTAGCGAACACTTTAATGAAGACTCAGATACACAAGAAACACAAGATGAAAAAATAAAGACAATAAAAGAAAAAATTTACGAACGCTACCAATTACACCTTGAAAGATCTCACTCCCTTGATTTTGATGATTTGTTGGTATTTGTATACAAATTATTTTATGAACCAAAATTCAGTTTTGTAGCTGAAAGATGA", "aa": "MIDLKDLNEQQKSAVIYNEGPLRIIAGAGSGKTRVLTYKIAYLIQKLGVLPNRILALTFSNKAANEMKQRVFNLLAEDNPSNLSPYISTFHAMCAKILRKEIYNFGYENDFQILDELDQKEILKIVYSELDISPTEFTFSSIISFIQNKKNSIEYLNNSISDSSEHFNEDSDTQETQDEKIKTIKEKIYERYQLHLERSHSLDFDDLLVFVYKLFYEPKFSFVAER", "aa_hexdigest": "bad450832414364d08d1a5526ce7d7f1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 199, "aa_cov": 0.827433628318584, "hmm_cov": 0.8944723618090452, "evalue": 3.003717794544331e-08, "score": 29.03215217590332, "start": 7, "stop": 193 }, { "id": "PF13245.12", "name": "AAA_19", "length": 119, "aa_cov": 0.5132743362831859, "hmm_cov": 0.6050420168067226, "evalue": 3.2947450197207425e-14, "score": 48.54956817626953, "start": 11, "stop": 126 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 218, "aa_cov": 0.9336283185840708, "hmm_cov": 0.9220183486238532, "evalue": 3.9062750809193394e-50, "score": 166.3082733154297, "start": 7, "stop": 217 } ], "seq_stats": { "molecular_weight": 26309.543500000025, "isoelectric_point": 5.243078804016112 }, "id": "AHCKFKLKGB_972", "locus": "GCA013367975_00971" }, { "type": "cds", "sequence": "contig_1", "start": 567854, "stop": 568057, "strand": "+", "frame": 2, "gene": null, "product": "3'-5' exonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00273A74DA" ], "nt": "ATGAATTTTGATAAGGATTACCCAAATTCAGTTACAGTAAAACTTGAAGAAAATTACAGATCAACCAAAACTATTTTAAGAGCAGCAAACAACTTAATTTCTCATAATAAACTTAGATTAGAAAAGAAATTATTCACCGAAAACGAAGAAGGCGAAGATATTGAGTTTTATTGTGGTTTTAATGACGAAGCAGAAGCCAGATGA", "aa": "MNFDKDYPNSVTVKLEENYRSTKTILRAANNLISHNKLRLEKKLFTENEEGEDIEFYCGFNDEAEAR", "aa_hexdigest": "97549997a635dfe7cb9efc2ab9e63df3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00273A74DA", "query_cov": 1.0, "subject_cov": 0.8701298701298701, "identity": 0.5670000000000001, "score": 73.9, "evalue": 5.36e-16, "valid": false, "product": "3'-5' exonuclease", "uniref50_id": "UniRef50_UPI00273A74DA" }, "pscc": { "uniref50_id": "UniRef50_UPI00273A74DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00273A74DA" ], "product": "3'-5' exonuclease" }, "genes": [], "id": "AHCKFKLKGB_973", "locus": "GCA013367975_00972" }, { "type": "cds", "sequence": "contig_1", "start": 568730, "stop": 569254, "strand": "+", "frame": 2, "gene": null, "product": "DNA 3'-5' helicase II", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F3QEJ6" ], "nt": "ATGACTGTTCACAACGCAAAAGGACTTGAATTTGATTATGTATTTGTTGCTTGCTTAGCAGAAAATATCTTTCCTCTAAAGAGGGCCATATCGATTTCTCCACAAGACGATTTTACTTTTTTAAATTCAAAAAATTTCAAAGAAAACATAGAAGGATTAGAGGAAGAAAGGCGTTTGGCTTATGTAGCAATGACTCGTGCTAAAAAAAGATTAATGCTTTCTTTCTCGGTTGGTAGAAATGGTATAAATAGAAAAAGTAGATTCTTGTCTGAAGCAGGAATAAACGAAATTAGAACAATAAAGATTGCCAACAACTTTTCAATTGCAGCAGAGCAAGAAACAAGAAATAACGATTTAATAGTCGGTGATTATATTATTCACAACACATATGGAAAAGGGTTGATTATAGATATGGTTGACAACGTTATTGAAGTTAAATTTGAAAATGATAAAAAGATTCGCAAATTATCAAAAACCCACCATTCAATTAAAAAATACGAAGATGACGAAAGTCTAAATAGTTAA", "aa": "MTVHNAKGLEFDYVFVACLAENIFPLKRAISISPQDDFTFLNSKNFKENIEGLEEERRLAYVAMTRAKKRLMLSFSVGRNGINRKSRFLSEAGINEIRTIKIANNFSIAAEQETRNNDLIVGDYIIHNTYGKGLIIDMVDNVIEVKFENDKKIRKLSKTHHSIKKYEDDESLNS", "aa_hexdigest": "f86494e5075fcec0840f21bd39e8d94d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0F3QEJ6", "query_cov": 0.9597701149425287, "subject_cov": 0.8835978835978836, "identity": 0.647, "score": 208.0, "evalue": 8.57e-66, "valid": false, "product": "DNA 3'-5' helicase II", "uniref50_id": "UniRef50_A0A0F3QEJ6" }, "pscc": { "uniref50_id": "UniRef50_A0A0F3QEJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F3QEJ6" ], "product": "DNA 3'-5' helicase II" }, "genes": [], "id": "AHCKFKLKGB_974", "locus": "GCA013367975_00973" }, { "type": "cds", "sequence": "contig_1", "start": 569700, "stop": 569882, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAATGTTTTCAACAAAGAAATTCATGATTTATCATATATTCCAACCATTGAAAATTATGAAGGTTTGTTTTTCTTTTTAAACAAGAAACACATTACTGATATAAATACTTTTTTAGACTTGTTTAAAGATTTAGGAAAGCAACACAATATTAACGGCTTAGAAGTTGTATATGAATGA", "aa": "MDNVFNKEIHDLSYIPTIENYEGLFFFLNKKHITDINTFLDLFKDLGKQHNINGLEVVYE", "aa_hexdigest": "6cbb567266ca09186c5843d8ecb41284", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7149.0074, "isoelectric_point": 4.923245811462402 }, "id": "AHCKFKLKGB_975", "locus": "GCA013367975_00974" }, { "type": "cds", "sequence": "contig_1", "start": 570153, "stop": 570614, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACATATGAAAACATTATTTATAACCTAAGAAATAATTTTTCTATTTCACTAAAAAATATTTTTTTAAAAAATGCAATTGGCGATAAAACCGAACTTAGAAGGTTTGGACTTCCTAAGATTTTTAATTCATTAAAATTAAACAAAGATTATGACTATTACCTAGAATTTTTAGACATAACTAGATTGTTTTATAATGTCTTTTACGAAAAAATCGGTGAACTACATCCTAAAAATGCAATATTAAAAATTAGTGATTCAATATTTGAAACATTAGATATAAACGTTATTAAAAAAATCGGGGTTGAATTTCCAACATTTATTCAATCAATAAAATTATTATCAGCAGAAAGAATAAAAGACATTTCCCAAAAAATTCTTAAACTTCAAGGAACAAAACTCAATTTTTGTCTAGAAATTAAGGATATTGATTCAACAATAATTTCTCTGGTATCCCCTTGA", "aa": "MTYENIIYNLRNNFSISLKNIFLKNAIGDKTELRRFGLPKIFNSLKLNKDYDYYLEFLDITRLFYNVFYEKIGELHPKNAILKISDSIFETLDINVIKKIGVEFPTFIQSIKLLSAERIKDISQKILKLQGTKLNFCLEIKDIDSTIISLVSP", "aa_hexdigest": "244b9d769adecf005ff54d02b07cbbeb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17876.7361, "isoelectric_point": 9.059629249572755 }, "id": "AHCKFKLKGB_976", "locus": "GCA013367975_00975" }, { "type": "cds", "sequence": "contig_1", "start": 570855, "stop": 571010, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAAACTTATTCCAATAGATTTCTTTGATGCAATAAATAAAAAGTGGGTAGATAGCCACAAGATCCCTGATTCTAAAAGCTCTATAGGTTGCTTTGCACAATTAGATAAAAATATTACCACATTAACTAAAAAAATATTAAATACATGA", "aa": "MNKKLIPIDFFDAINKKWVDSHKIPDSKSSIGCFAQLDKNITTLTKKILNT", "aa_hexdigest": "c743cad281ddbbf0caba1dccb90291c7", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5820.7789999999995, "isoelectric_point": 9.519159889221193 }, "id": "AHCKFKLKGB_977", "locus": "GCA013367975_00976" }, { "type": "cds", "sequence": "contig_1", "start": 571182, "stop": 571349, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTGAAATTATCTAGTTTAAACACCCCTTTAATTTTTTCAGTTTCAACAGATTTTAAAAACAGCGATGTTCAAACTCTTTATTTAGATGTAAACTCTACAATTCTACCAGACAAAAGTTTTTATACTGATCCTGTTAAAAAAGAAAAATTTATTAATGTTTGA", "aa": "MELKLSSLNTPLIFSVSTDFKNSDVQTLYLDVNSTILPDKSFYTDPVKKEKFINV", "aa_hexdigest": "c39139b218287a9536ebb866d6be4468", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6285.128500000001, "isoelectric_point": 4.983893013000488 }, "id": "AHCKFKLKGB_978", "locus": "GCA013367975_00977" }, { "type": "cds", "sequence": "contig_1", "start": 571482, "stop": 572156, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAAAATTTGCAATACTATTTCAACAAAAGAATTAGCCCAAAACGTTAAAACAATAAACATAGAAAATATATTAAGACAATTAATTAAATGTTTCCCCGAAGAAATAAATGCTATTGATCCAAATTCTATAAACATTGCAAAATCTTTAACTAATGAAGAAAATTTTGAATATTATAAAGCAAGTTTAATTATTGATGCAATATTAACCTATGCACCTTATTTCGATTATTCAACAATTCAAATTGCTTCTGAATTTAATTTAGCAATAAACGGAAACAAAAAAATTGAATCAAAAGAAAAATATCAAATTAAACAAGCAAAACATTTTTTTGATATTCCTTTTGGAACTTACTATGGTAAAACCTATTTTGGATTAAAAAACAAGCAAACGGTCGAACACATGATAAAAAGCATGATAGGCGTTTATAAAGAACAGCTAGCAAAAAACTCTTGGTTAAGTAAACAAACAAAAGAAAAAGCAATAACAAAGCTTAACTCAATCGGTGTTTATGTTGGTTTTCCTGAAATAATTCAAGATCACTATAAATATTTAATAGTTGAAAAATTTGATGGATACAATGATTTATTTGAAAACGTTTTAAAGTTTAACAAAATTTTAGCTGAACACATTTTATCTGAATACGGAAAAACAGAAGACAAAAACCTTTGA", "aa": "MVKICNTISTKELAQNVKTINIENILRQLIKCFPEEINAIDPNSINIAKSLTNEENFEYYKASLIIDAILTYAPYFDYSTIQIASEFNLAINGNKKIESKEKYQIKQAKHFFDIPFGTYYGKTYFGLKNKQTVEHMIKSMIGVYKEQLAKNSWLSKQTKEKAITKLNSIGVYVGFPEIIQDHYKYLIVEKFDGYNDLFENVLKFNKILAEHILSEYGKTEDKNL", "aa_hexdigest": "9d0e5175ce5f3e105c2486e92d501a81", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26001.61420000002, "isoelectric_point": 8.259963417053225 }, "id": "AHCKFKLKGB_979", "locus": "GCA013367975_00978" }, { "type": "cds", "sequence": "contig_1", "start": 572160, "stop": 572369, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACCTGCTATGGTTAATGCTTATTATAATCCTTCAAAAAATGTAATAGTATTCCCTGCCGCAATCTTACAAAAACCTTTCTTTAGTGCAGATCAATCCTCTTCATCAAATTTTGGTGGAATTGGTGCTGTTATTGCTCATGAAATATCACACGGATTTGACAATAACGGAGCAAATTTCGATGAAAAAGGAAATATGATTAATTGA", "aa": "MSPAMVNAYYNPSKNVIVFPAAILQKPFFSADQSSSSNFGGIGAVIAHEISHGFDNNGANFDEKGNMIN", "aa_hexdigest": "6ebac6a6f366996c081c11a5317a49ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7351.097399999999, "isoelectric_point": 5.337602043151855 }, "id": "AHCKFKLKGB_980", "locus": "GCA013367975_00979" }, { "type": "cds", "sequence": "contig_1", "start": 572415, "stop": 572585, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATTTGTTCGAAGGTAGAGAAACATCTGTTGGTAAATGTAACGGTACATTAACTGTTTCAGAAAACATAGCAGATGCTGGTGGTTTATCATGTGCTTTAGCAGCGGCAAAATTAGAAAAAGATTATAACCCTAAAGATTTTTATATAAATTTTGCAACAATTTGA", "aa": "MINLFEGRETSVGKCNGTLTVSENIADAGGLSCALAAAKLEKDYNPKDFYINFATI", "aa_hexdigest": "8a342e6c8964b587198068ef7141ae43", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5986.715900000001, "isoelectric_point": 4.715044593811036 }, "id": "AHCKFKLKGB_981", "locus": "GCA013367975_00980" }, { "type": "cds", "sequence": "contig_1", "start": 572619, "stop": 572759, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAACTATTGATTGTCACGCTCCTGCTAAATTAAGAACAAATGTTCAAATACAAAACTCTGATGATTTTTATACAACTTTCAATGTCAAAAAAGGTGACCCAATGTATTTGCCACCTGAAAAAAGAGTAAAAATTTGA", "aa": "MLTIDCHAPAKLRTNVQIQNSDDFYTTFNVKKGDPMYLPPEKRVKI", "aa_hexdigest": "b2da131b05c2e6442b6d66a0bc5641e4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5338.145600000001, "isoelectric_point": 8.931272697448733 }, "id": "AHCKFKLKGB_982", "locus": "GCA013367975_00981" }, { "type": "cds", "sequence": "contig_1", "start": 572802, "stop": 573713, "strand": "-", "frame": 2, "gene": "tmcAL", "product": "nucleotidyltransferase", "db_xrefs": [ "COG:COG1323", "COG:J", "EC:6.3.1.22", "KEGG:K27343", "RefSeq:WP_175933582.1", "SO:0001217", "UniParc:UPI00158AB184", "UniRef:UniRef100_UPI00158AB184", "UniRef:UniRef50_A0A3M0A6W2", "UniRef:UniRef90_D1J8P3" ], "nt": "ATGAAAATAGGAATAATAGCTGAATTTAACCCATTTCACAATGGGCATATTTATTTAATTAATAAAATTAAAGAAATCTTCAAAGATCCTGAAATAATTGTTGCATTATCTTGCGATTATGTTCAACGGGGCGAAATAGCATGTTTACCTTTTGAAGTTAGAAAAAACATTGCGCTTGAATATGGAGCAACAAAAGTTGTTGAATTAGACTTTTTTGCATCTACACAAGCAGCCCATATTTTTGCTAAAAAATCAATAGATTTGTTAATAAAAGAAGGTATTGATTATTTAGTTTTTGGGGTTTCTGACACTGATGATATAAAAAAATATTTAAACGCAGCAAACGTAATAAAGAAAAATTTTGATCAATATAACAAAGATGTAAGAATGAATTTAAAAACAGGAAAATCATATGTTTTGAGTTGTTTCTTGTCGCTAGAAAAATTAATAGGTGTTGAAAATATACCACAAGATATTTTGGGGTTTGAATATACCAAATACATAGTTTTTAATAATTTGTCAGTAAAACCGTTTTGCATAAAAAGAACAGCTCCACACAATTCGTTAATAGCAAATAATAATTATGCTTCGGCAACAATGATACGAAAAATGTTAGAAGAAGGCGAAGATGTTTCAATGTTCACACCGATAAACATTCCTAAAAATTTTTCAAAAATTGAAGATAAATATAATGAGTTTAAACAAAAGGTACAAAATTATTCTAAAGAAGAATTAGCAAATATAGCATTGATATCCGAAGGAATGGAAAATCTTTTTAAAAAGAATATTGAAATAGCAAAAAATTATAATGAATTTGTTGATTTATGTACTTCGAAAAGATATACAAATAGCAGAATTAAACGAGTGATGTTATATGTTCTTTTAGGAATAAAGAAAGATGATTTGTGTTAA", "aa": "MKIGIIAEFNPFHNGHIYLINKIKEIFKDPEIIVALSCDYVQRGEIACLPFEVRKNIALEYGATKVVELDFFASTQAAHIFAKKSIDLLIKEGIDYLVFGVSDTDDIKKYLNAANVIKKNFDQYNKDVRMNLKTGKSYVLSCFLSLEKLIGVENIPQDILGFEYTKYIVFNNLSVKPFCIKRTAPHNSLIANNNYASATMIRKMLEEGEDVSMFTPINIPKNFSKIEDKYNEFKQKVQNYSKEELANIALISEGMENLFKKNIEIAKNYNEFVDLCTSKRYTNSRIKRVMLYVLLGIKKDDLC", "aa_hexdigest": "40e04c033ba4009d92fc570cbd60d25f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158AB184", "ncbi_nrp_id": "WP_175933582.1", "uniref100_id": "UniRef100_UPI00158AB184", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158AB184", "RefSeq:WP_175933582.1", "UniRef:UniRef100_UPI00158AB184" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158AB184", "uniref90_id": "UniRef90_D1J8P3" }, "psc": { "uniref90_id": "UniRef90_D1J8P3", "gene": "TmcAL", "product": "nucleotidyltransferase", "ec_ids": [ "6.3.1.22" ], "uniref50_id": "UniRef50_A0A3M0A6W2", "cog_id": "COG1323", "cog_category": "J", "kegg_orthology_id": "K27343" }, "pscc": { "uniref50_id": "UniRef50_A0A3M0A6W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3M0A6W2" ], "product": "tRNA(Met) cytidine acetate ligase" }, "genes": [ "tmcAL" ], "id": "AHCKFKLKGB_983", "locus": "GCA013367975_00982" }, { "type": "cds", "sequence": "contig_1", "start": 573774, "stop": 573929, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA58", "UniRef:UniRef90_A0A454CA58" ], "nt": "ATGTTTAATAAAAAGAAAACAAGCAAGAAAAAACAAATAAAAAAAGAAAAAGAACAAAGCGATATTGTGATGTTCGAAGATTTTGATTCAAACAAAAATTGTGAAGGCGATCAAAAAAACATTGAGATCCAAGAAACTGTGGAATTAAATTATTGA", "aa": "MFNKKKTSKKKQIKKEKEQSDIVMFEDFDSNKNCEGDQKNIEIQETVELNY", "aa_hexdigest": "b74159c3055ddb16e4b2c8f7ccc2c8b8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 573714, "stop": 573929, "observations": { "mutation": [ 573776 ], "stop": [ 573776 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA58", "query_cov": 1.0, "subject_cov": 0.7183098591549296, "identity": 0.902, "score": 84.0, "evalue": 2.56e-20, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MLNKKKTNKKKQIKKEKEQSDIVMFEDFDSNKKSEDDQKNIEIQETVELNYWEDSSIEDFCDISLDELKKK" }, "paralog": false, "identity": 0.9154929577464789, "score": 129.0, "evalue": 4.39e-41, "description": "Internal stop codon at 573776. Nonsense mutation around 573776.", "psc": { "uniref90_id": "UniRef90_A0A454CA58", "uniref50_id": "UniRef50_A0A454CA58", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA58", "UniRef:UniRef50_A0A454CA58" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454CA58", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA58" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6129.858499999999, "isoelectric_point": 6.219856834411621 }, "id": "AHCKFKLKGB_984", "locus": "GCA013367975_00983" }, { "type": "cds", "sequence": "contig_1", "start": 574150, "stop": 574359, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAATATTCAAGATACAATAGTTGCTATTTCTTCAGGCAATGTAAATCAAGCAATATCTATTATCAGATTATCTGGTGAGGATTCAATTGAGATTGTTAAAAAAGTTTTTAGTGGTAAAGTCGGAAAAGATAAGACAATTACTTACGGAAATATCATCGATAATATAACAGGGAAAATTGTTGATGAGGTATTAGTTAATTGA", "aa": "MKNNIQDTIVAISSGNVNQAISIIRLSGEDSIEIVKKVFSGKVGKDKTITYGNIIDNITGKIVDEVLVN", "aa_hexdigest": "d194803feaaad9dfd533e40e869ae95f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7432.4607, "isoelectric_point": 6.099699211120604 }, "id": "AHCKFKLKGB_985", "locus": "GCA013367975_00984" }, { "type": "cds", "sequence": "contig_1", "start": 574471, "stop": 575226, "strand": "+", "frame": 1, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "COG:COG0486", "COG:J", "GO:0003924", "GO:0005525", "GO:0006400", "SO:0001217", "UniRef:UniRef50_A0A077L8N2", "UniRef:UniRef90_D1J8P4" ], "nt": "TTGGCAAACAGAGGAGAATTTACAAGACGAGCATTTTTAAACGGAAAAATTTCGTTAGATAAAGCCGAAGCGATAAATAGCTTAATTCATGCAAAAACAAATAAACAAGCAGAAATTGCTATTTCTCAATTTAATTCTAAGGACAATTTAATTATTGAAAATTTAATTAACGAACTACTTACTATAATTTCATTAATCGAAATTAATATTGATTATTCTGATTATAACGATATAAAAGAAATGGATGCTAACACCTTAACAAGTCAATTATCAAAATTTAATAGAAAATTAGATTCTATAATTAATAAATCAGAAAATGCTTGCGATATTTATAAAGGAATAAATGTTGCGATAGTAGGAAGACCCAACACTGGTAAATCATCACTTTTAAATTCTTTAATTGGTTCACAAAAAGCAATAGTAACAAATATACCCGGAACAACAAGAGACGTGGTAGAAGGCGAATTTCAGATTAATGGTTTTTTATTTAATTTGATTGATACAGCGGGTATAAGACAAACAAAAGATCTTGTTGAATCAATAGGTATTGAAAGATCTAAAAACGCAATAAAAGAAGCCAAAATTGTAATTCATATGCACGAACCATTTATTAAAGAAAATGAAGAAGATCAAATAATTAAAGAACTCTCAAAAGGTAAGGTATATATACCGGTAATGAACAAGCAAGATCTTTTAAACAAAGATCAATATCCTAAGAACATGGTTTTAATCTCAAGCAAAGAAAATAGTCTATGA", "aa": "MANRGEFTRRAFLNGKISLDKAEAINSLIHAKTNKQAEIAISQFNSKDNLIIENLINELLTIISLIEINIDYSDYNDIKEMDANTLTSQLSKFNRKLDSIINKSENACDIYKGINVAIVGRPNTGKSSLLNSLIGSQKAIVTNIPGTTRDVVEGEFQINGFLFNLIDTAGIRQTKDLVESIGIERSKNAIKEAKIVIHMHEPFIKENEEDQIIKELSKGKVYIPVMNKQDLLNKDQYPKNMVLISSKENSL", "aa_hexdigest": "067c560be863f2b5f078405603c959e4", "start_type": "TTG", "rbs_motif": null, "genes": [ "mnmE" ], "truncated": "both", "pseudogene": { "start": 574171, "stop": 575496, "observations": { "mutation": [ 574357, 575224 ], "stop": [ 574357, 575224 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8P4", "query_cov": 1.0, "subject_cov": 0.5602678571428571, "identity": 0.9840000000000001, "score": 466.0, "evalue": 1.62e-162, "gene_start": 1, "gene_end": 251, "reference_start": 108, "reference_end": 358, "reference_sequence": "MKNNIQDTIVAISSGNVNQAISIIRLSGEDSIEIVKKIFSGKVGKDKTITYGNIIDNITGKIVDEVLVNWFIGNKNYTGENTVEINAHGGIVVTNKILNLLIANGARLANRGEFTRRAFLNGKISLDKAEAINSLIHAKTNKQAEIAISQFNSKDNLIIENLINELLTIISLIEINIDYSDYNDIKEMDANTLTNQLLKFNRKLDSIINKSENACDIYKGINVAIVGRPNTGKSSLLNSLIGSQKAIVTNIPGTTRDVVEGEFQINGFLFNLIDTAGIRQTKDLVESIGIERSKNAIKEAKIVIHMHEPFIKENEEDQIIKELSKGKVYIPVMNKRDLLNKDQYPKNMVLISSKENSLWELKNALVKNYLNIDLDDPQIIFNNRKLSLLKQAKISINTALESLSQGLGPEVVILDINEAWGSLSEILNKKHDNEALLDNIFSKFCLGK" }, "paralog": false, "identity": 0.9863945578231292, "score": 835.0, "evalue": 9.29e-311, "description": "Internal stop codon at 574357, 575224. Nonsense mutation around 574357, 575224.", "psc": { "uniref90_id": "UniRef90_D1J8P4", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "uniref50_id": "UniRef50_A0A077L8N2", "cog_id": "COG0486", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006400" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8P4", "UniRef:UniRef50_A0A077L8N2", "COG:COG0486", "COG:J", "GO:0003924", "GO:0005525", "GO:0006400" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L8N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L8N2" ], "product": "tRNA modification GTPase MnmE" } }, "id": "AHCKFKLKGB_986", "locus": "GCA013367975_00985" }, { "type": "cds", "sequence": "contig_1", "start": 575257, "stop": 575496, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATATTGATTTAGATGACCCTCAAATAATTTTTAATAATAGAAAACTTTCGTTATTGAAGCAAGCGAAGATTTCTATCAATACTGCATTAGAATCGCTAAGTCAAGGTTTGGGCCCTGAGGTGGTTATTTTAGATATAAACGAAGCTTGGGGGTCATTGAGTGAAATATTAAATAAAAAGCACGATAATGAAGCTCTACTAGATAATATATTTAGCAAATTTTGTTTAGGAAAATAA", "aa": "MNIDLDDPQIIFNNRKLSLLKQAKISINTALESLSQGLGPEVVILDINEAWGSLSEILNKKHDNEALLDNIFSKFCLGK", "aa_hexdigest": "3f43011272382563ccd98ba544c3b800", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8809.040799999993, "isoelectric_point": 4.9024995803833 }, "id": "AHCKFKLKGB_987", "locus": "GCA013367975_00986" }, { "type": "cds", "sequence": "contig_1", "start": 575498, "stop": 576052, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAAAATAAACGATAAAATAATTGTAAAAGCAACAGAACTAAGTTATGAAGGCTATGGGGTTGTGAGAGGTGATAAACAAACACTCTTTGTTGAAAATCTCTTACCTGATGAAGAAGCAGAAGTAGCCATTTATAAATCAAATTCAAAAATTGCTTATGGACGTACAGTTAATTTGATAAAAAAATCATGTAAAAGAGTTGAAGTTGTTGAGAAAGAATTGTTTGAAAGTGGTGCAGCGCCATTGATGGTTTTAAGTTACGAAGATCAATTAAAATTCAAACAAGAAATTGTTAATAATTTAATAAAAAGAAATATTGGATTTCAAAACATTGAACAAATTCAACCTAGTCCAAAAATATTAAATTACAGAAATAAAATAACCTTACATGTGAATTATAAAAACAATAATATTTATATTGGTTTCTATAAAAAAAACAGCAACAAACTAGTTATTCAAAATTCGCTTCCTCTTTGTCATGATGAACTTGATAAATATTACAAGGAACTTATTAGCGCCCTAAAACAACAAATTTTATCAAAAGATAATTGA", "aa": "MYKINDKIIVKATELSYEGYGVVRGDKQTLFVENLLPDEEAEVAIYKSNSKIAYGRTVNLIKKSCKRVEVVEKELFESGAAPLMVLSYEDQLKFKQEIVNNLIKRNIGFQNIEQIQPSPKILNYRNKITLHVNYKNNNIYIGFYKKNSNKLVIQNSLPLCHDELDKYYKELISALKQQILSKDN", "aa_hexdigest": "49d9445ec8737b12a3b36aca005ae468", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21353.422400000025, "isoelectric_point": 9.09727878570557 }, "id": "AHCKFKLKGB_988", "locus": "GCA013367975_00987" }, { "type": "cds", "sequence": "contig_1", "start": 576158, "stop": 576829, "strand": "+", "frame": 2, "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "db_xrefs": [ "COG:COG2265", "COG:J", "EC:2.1.1.190", "GO:0008649", "KEGG:K03215", "SO:0001217", "UniRef:UniRef50_D1J8P5", "UniRef:UniRef90_D1J8P5" ], "nt": "TTGTTGAAAAATAATTTGATTTTCTTATTGAAACCCAATGTAATTTCAATACAATGCCAATATATTAAAGAATCTAAAGATATATTCACTTCTTTGTATAAATCAATGCCCCAACAATTTAATATAAACAATAAAATATTCAATGTGAACAATGATTCTTTTTTTCAAGTAAATGAAGAACAAACTAGTTTAATATATAATCAAATTAAAAAATTCATAGATCAAGAAAATTCAACAAAAATAATTGATGTTTATTCAGGTGTTGGTAGCATTGGAATATCTGTTTGTGGCAATGACTCTCAACTTGAATTAATTGAAATAAATAAATCGGCGTGTGAGTGTGCAAAAATTAACATAAACCAAAATTTGATTAATCCAAAACTAGCGGATATAACAAATAGTTCAGCACAAGACTTTTTTAATAAAAAAGAAGCAGAATATTTTGAAAAATCGATAGTTATATTTGACCCACCAAGAACGGGTTTAACAAATAAAGAAATTGATATGCTAGAAAAATGCAATAGTGTAATTTATATGAGTTGCAATCCTCATACAATGATTAGGGATTTAAAATATTTTGAAGAAAAAGGGTATAGAATCGAAAGGCTAATACCTTATGATATGTTTCCTCAAACTTATCATATTGAAATGTTGTCGCTTTTAAAAAAATAA", "aa": "MLKNNLIFLLKPNVISIQCQYIKESKDIFTSLYKSMPQQFNINNKIFNVNNDSFFQVNEEQTSLIYNQIKKFIDQENSTKIIDVYSGVGSIGISVCGNDSQLELIEINKSACECAKININQNLINPKLADITNSSAQDFFNKKEAEYFEKSIVIFDPPRTGLTNKEIDMLEKCNSVIYMSCNPHTMIRDLKYFEEKGYRIERLIPYDMFPQTYHIEMLSLLKK", "aa_hexdigest": "bf28e169d884a5114094a5957959d75a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rlmD" ], "truncated": "5-prime", "pseudogene": { "start": 575858, "stop": 576829, "observations": { "mutation": [ 576050, 576098 ], "stop": [ 576050, 576098 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8P5", "query_cov": 1.0, "subject_cov": 0.41527001862197394, "identity": 0.978, "score": 436.0, "evalue": 9.89e-150, "gene_start": 1, "gene_end": 223, "reference_start": 221, "reference_end": 443, "reference_sequence": "MYKINDKIVVKATELSYEGYGVVRSDKQTLFVENLLPREEAEVAIYKSNSKIAYGRTVNLIKKSCKRVEVVEKELFESGAAPLMVLSYEDQLKFKQEIVNNLIKRNIGFQNIEQIQPSPKILNYRNKITLHVNYKNNNIYIGFYKKNSNKLVIQNSLPLCHDELDKYYKELISALKQQILSKDNWIKELKLRQITLRHSNWSHNIEVIFIIENPNKKGFDLLKNNLIFLLKPNVISIQCQYIKESKDIFTSLYKSMPQQFNINNKIFNVNNDSFFQVNEEQTSLIYNQIKKFIDQENSTKIIDVYSGVGSIGISVCGNDSQLELIEINKSACECAKININQNLINPKLADITNSSAQDFFNKKEAEYFEKSIVIFDPPRTGLTNKEIDMLEKCNSVIYMSCNPHTMIRDLKYFEEKGYRIERLIPYDMFPQTYHIETVALLSKLDVDKHINIEIELDELDLTSAESKATYAQIKEYVWNKFELKVPTLYIAQIKRKCGIELREHYNKSKKEKQIIPQCTPEKEEAIMDALRHFKMIE" }, "paralog": false, "identity": 0.9814241486068112, "score": 637.0, "evalue": 2.84e-231, "description": "Internal stop codon at 576050, 576098. Nonsense mutation around 576050, 576098.", "psc": { "uniref90_id": "UniRef90_D1J8P5", "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "ec_ids": [ "2.1.1.190" ], "uniref50_id": "UniRef50_D1J8P5", "cog_id": "COG2265", "cog_category": "J", "kegg_orthology_id": "K03215", "go_ids": [ "GO:0008649" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8P5", "EC:2.1.1.190", "UniRef:UniRef50_D1J8P5", "COG:COG2265", "COG:J", "KEGG:K03215", "GO:0008649" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8P5" ], "product": "TRAM domain-containing protein" } }, "id": "AHCKFKLKGB_989", "locus": "GCA013367975_00988" }, { "type": "cds", "sequence": "contig_1", "start": 577079, "stop": 577375, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCTTTGGGTTATGTATCAAGCGCAATTGTTGCAAACGAAATTGGAAAACAAAATTTTGAAGCTGCTCATGAACTCGGAAATAGTGCATTAAAGTTTGGCTTTTATGCACAGTCATTATTTTCAATATTAGGAGTTTCATTAACCTGGCCTTTGCTAAGACTTTATTCAATAGATATTCAAATAATTAATTCTGTTGGGTATGGTGTTATGGGAATTATGATGCTAAGAGCTATTCTGGATATAGGAAACCTTACAACCCTAAGGGCTTTATGGGGCGCCAATGATGTTTGA", "aa": "MIALGYVSSAIVANEIGKQNFEAAHELGNSALKFGFYAQSLFSILGVSLTWPLLRLYSIDIQIINSVGYGVMGIMMLRAILDIGNLTTLRALWGANDV", "aa_hexdigest": "a0f7b2932e5f795e14d6b83c3ad40064", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10622.31879999999, "isoelectric_point": 5.564105415344237 }, "id": "AHCKFKLKGB_990", "locus": "GCA013367975_00989" }, { "type": "cds", "sequence": "contig_1", "start": 577425, "stop": 577604, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTGTTCATTCTTTCCTTCGAACCCCATCGGAATTGCTCTTGCAAGAAGATATTGTCCTATGAATCAAATTCCTGTTTCAAAAGCAATAGGAAGACCTAACTTAAGAATGTTTTTAACATATTTTGTGTTTCATGTTTCTTTAATTGAAATTTTATTGTTTTTGGTTATAAAGTGA", "aa": "MNCSFFPSNPIGIALARRYCPMNQIPVSKAIGRPNLRMFLTYFVFHVSLIEILLFLVIK", "aa_hexdigest": "4af00f4994d5348eaad36d0ded4f4ccf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6802.214399999995, "isoelectric_point": 10.051926612854004 }, "id": "AHCKFKLKGB_991", "locus": "GCA013367975_00990" }, { "type": "cds", "sequence": "contig_1", "start": 577724, "stop": 578008, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATCACAACATCACTATAAATGATTTAGCTAGAATGAATCAATTTAATAAGGTGCTATACGGTGGAACTATAAATGGAATTACCCTAGTTAATACAAATCCTTTTAAGGTTTCAGTAACTTCCGAAACAACAATGGCTATAAAATTCTTAAGAATAACCACTTGCGACATATTCATATATTCAATAGCCGTTATTTTTACGGCCAGTTTACAATCTGTTGAAAAAAATAAATTTTCAATATTTGGATTAATTACCAGTATATTTGTACGTGCAAGTTGA", "aa": "MKNHNITINDLARMNQFNKVLYGGTINGITLVNTNPFKVSVTSETTMAIKFLRITTCDIFIYSIAVIFTASLQSVEKNKFSIFGLITSIFVRAS", "aa_hexdigest": "723e38f4e0c9e5227a8e43db31948215", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10498.180399999992, "isoelectric_point": 9.80527057647705 }, "id": "AHCKFKLKGB_992", "locus": "GCA013367975_00991" }, { "type": "cds", "sequence": "contig_1", "start": 578054, "stop": 578533, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAGAAAGAAAACAGTAAACAAAGAAATACAAATCGTTTAAGTATTTTTATTGAAAAATCAAGTGAATTATTTAAAGTTAAACACTTTAAGTATATTTTCAAATTGACTTTACCAATCTTTTTTCAGACACTTTTTGTTGCTTTAGTATCGGTAGTTTCTTCTCTTGCTTCTTCGCATTATATAAAAGTCTATCACGCCGATGGTTCATACAATGGTTTTTATTTTTATATGATTGCAAAAATATTAACTGTTTACAAAATGATAACCTTTATTCCTATTATTTACCAAAGCGGAACATTAGTCTTATGCTCAAATCTTTTTGGACAAAGCAAAATAAGAGACATACCAAAAGCTTTGTGGTCTGCCGTTTATGTATCATTAATAATAAATACTTGTGTCTATTTTATTATGTTTGGACTTGCACCAATAGTTTTACAAGCTTCTGGAACAAAAAACATTCCTCTTTTTGGTTGA", "aa": "MRKKENSKQRNTNRLSIFIEKSSELFKVKHFKYIFKLTLPIFFQTLFVALVSVVSSLASSHYIKVYHADGSYNGFYFYMIAKILTVYKMITFIPIIYQSGTLVLCSNLFGQSKIRDIPKALWSAVYVSLIINTCVYFIMFGLAPIVLQASGTKNIPLFG", "aa_hexdigest": "db464adc4046dff1ab82c559d7447749", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18207.470799999992, "isoelectric_point": 9.869481086730957 }, "id": "AHCKFKLKGB_993", "locus": "GCA013367975_00992" }, { "type": "cds", "sequence": "contig_1", "start": 578825, "stop": 579217, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28", "PFAM:PF02881.24" ], "nt": "TTGCAAAACAAACATAATAAAATTGACGAAGAATTTTTTGAAGATTTGGAAGAAATGCTGATTATGTCTGATATTTCGATTAAGCTAGTGCAAATAATTATTAACGAATGTAAAAAAGAAGTTAGAAACGAAAATATAACTGATCCAAAATTAATAAACGAGATAATTGCTGACAAACTGTTTACAATTTATACATCAAATTCTGTTGTCGATACAACACTTAATATTAAAGATAATAGGTTAAATGTAATATTGGTTGTGGGCGTCAATGGTTCAGGAAAAACTACATCAATATCAAAAATTGCTAAGAAATTAATTGATGAAGGTAAAAAAGTATTGATTGCTGCAGGAGATACATTTAGAGCAGCAGCAGTTGAGCAATTAGAGATTTGA", "aa": "MQNKHNKIDEEFFEDLEEMLIMSDISIKLVQIIINECKKEVRNENITDPKLINEIIADKLFTIYTSNSVVDTTLNIKDNRLNVILVVGVNGSGKTTSISKIAKKLIDEGKKVLIAAGDTFRAAAVEQLEI", "aa_hexdigest": "32b482f41e01095d3ff1d7245a548d38", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 48, "aa_cov": 0.36923076923076925, "hmm_cov": 1.0, "evalue": 1.4791922213792734e-20, "score": 69.00234985351562, "start": 82, "stop": 129 }, { "id": "PF02881.24", "name": "SRP54_N", "length": 62, "aa_cov": 0.43846153846153846, "hmm_cov": 1.0, "evalue": 4.6377491550681176e-15, "score": 50.97073745727539, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 14575.721299999977, "isoelectric_point": 5.072220802307128 }, "id": "AHCKFKLKGB_994", "locus": "GCA013367975_00993" }, { "type": "cds", "sequence": "contig_1", "start": 579263, "stop": 579688, "strand": "+", "frame": 2, "gene": "ftsY", "product": "Signal recognition particle-docking protein FtsY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH26" ], "nt": "GTGGATCCGGCGGCGGTTGTTTATAGGGCAATCGATATAGCAAAATCAAAAAAATACGATATATTGATTGTAGATACTGCGGGAAGGTTGCAAAATAAAGTAAATTTAATGAACGAACTTGCCAAAATCAATAGGGTTTTAGCCTCTAAAATTCCCGATGCACCTCATGAAAGTTTATTAGTTTTAGATGCAACAACCGGACAAAATGGTGTAATTCAGGCAAAGGTATTCAAGGAATCCACACCACTGACCGGAATCATTCTAACAAAAATGGACGGAACCTCAAAAGGTGGAATTGTTTTAACAATCAAAGATGAGATTGGTTTGTTTGTAAAATACATTGGTTTAGGTGAAAAAGTTGAAGATTTAGCGGAATTTGATTTAGATTCTTATATCTATGGTTTAACAAAAGGTATTAACGAATAA", "aa": "MDPAAVVYRAIDIAKSKKYDILIVDTAGRLQNKVNLMNELAKINRVLASKIPDAPHESLLVLDATTGQNGVIQAKVFKESTPLTGIILTKMDGTSKGGIVLTIKDEIGLFVKYIGLGEKVEDLAEFDLDSYIYGLTKGINE", "aa_hexdigest": "77af9754b2e3cd5b9b648677175cda08", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AH26", "query_cov": 0.9361702127659575, "subject_cov": 0.8407643312101911, "identity": 0.705, "score": 191.0, "evalue": 5.88e-60, "valid": false, "product": "Signal recognition particle-docking protein FtsY", "uniref50_id": "UniRef50_A0A449AH26" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH26" ], "product": "Signal recognition particle-docking protein FtsY" }, "genes": [ "ftsY" ], "id": "AHCKFKLKGB_995", "locus": "GCA013367975_00994" }, { "type": "cds", "sequence": "contig_1", "start": 579688, "stop": 579912, "strand": "+", "frame": 1, "gene": "ylxM", "product": "putative DNA-binding protein YlxM, UPF0122 family", "db_xrefs": [ "COG:COG2739", "COG:K", "KEGG:K09787", "RefSeq:WP_020002601.1", "SO:0001217", "UniParc:UPI00031E45B5", "UniRef:UniRef100_A0A2K9YTY8", "UniRef:UniRef50_C4XE46", "UniRef:UniRef90_A0A2K9YTY8" ], "nt": "ATGGAAACAATTGAAGAACGAGATTACTATGTAAAGTTGTTTGAAAAATATGGTGATTTATTACCACAATCTCAAAAACAAGCTCTACAATTACTTTTATATGAAGATTTGTCATACGGGGAATTAGCCCAAGAATTAGCGATGTCTAGACCAGGGGCATACGACGCAGTTAAAAAAGCAAAATTGCACTTAAAACAAATTGATGAAAAGTTAAAACAAAGTTAA", "aa": "METIEERDYYVKLFEKYGDLLPQSQKQALQLLLYEDLSYGELAQELAMSRPGAYDAVKKAKLHLKQIDEKLKQS", "aa_hexdigest": "78c4178b8a69d62122a90a1d2b675034", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00031E45B5", "ncbi_nrp_id": "WP_020002601.1", "uniref100_id": "UniRef100_A0A2K9YTY8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031E45B5", "RefSeq:WP_020002601.1", "UniRef:UniRef100_A0A2K9YTY8" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTY8", "uniref90_id": "UniRef90_A0A2K9YTY8" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YTY8", "gene": "YlxM", "product": "Predicted DNA-binding protein YlxM, UPF0122 family", "uniref50_id": "UniRef50_C4XE46", "cog_id": "COG2739", "cog_category": "K", "kegg_orthology_id": "K09787" }, "pscc": { "uniref50_id": "UniRef50_C4XE46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE46" ], "product": "Sigma-70, region 4" }, "genes": [ "ylxM" ], "id": "AHCKFKLKGB_996", "locus": "GCA013367975_00995" }, { "type": "cds", "sequence": "contig_1", "start": 580012, "stop": 580179, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTATAAAAGAGTATTGATTAAACTTTCGGGTGAGGGCTTAGCAAATAAAGGTAAACACCTTGCAATTGATTATGAATTGGTTGAAAAATTTGCAAACCAATTAAAAACAATAATTAGTAGAAATATTGAAGTAGCGATTGTTGTCGGCGGCGGTAATTTTTGA", "aa": "MSYKRVLIKLSGEGLANKGKHLAIDYELVEKFANQLKTIISRNIEVAIVVGGGNF", "aa_hexdigest": "6d8fef6658cc5c79d62692c6239603c9", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6047.0342, "isoelectric_point": 9.52193202972412 }, "id": "AHCKFKLKGB_997", "locus": "GCA013367975_00996" }, { "type": "cds", "sequence": "contig_1", "start": 580207, "stop": 580740, "strand": "+", "frame": 1, "gene": null, "product": "UMP kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "nt": "ATGCAAAGGGTTAGAGCAGATTACATTGGTATGCTAGCTACAACAATGAATGCACTAGCTTTGCAATCAGGATTTGAGAGATTGAATTTGCATTGTAGAGTATTATCAAGCCTAACAATGGACCCAAAGGTTTGTGAAGTTTATATAAATGAAAAAGCTAAAAAATACCTAAGAGATGGAGAAGTTGTAATTTTTGCCGGGGGAACTGGACGCCCATTTTTTACAACTGATACAGCCTCAACATTATTTGCCAGTGAAATTGATGCGGATGCAATATTGATGGGAAAAAATAATGTTGATGGGGTATATGATAGTGATCCAAAGTTCAATAAAGACGCCAAAAAATTTCCAATCATTACATATGATGAATTGATAGAAAAGGAATTAAAAGTTATTGATCAAACCGCAGCATCAATGGCAAAAGAAAATAATATTGATATTTTAATATTTGATATTAATGAAGAAGATTCTTTAATAAGGGCAATAGAAAATAAAATACCTAACACAATAATAACTAGAGAAAGGGAAAAATAA", "aa": "MQRVRADYIGMLATTMNALALQSGFERLNLHCRVLSSLTMDPKVCEVYINEKAKKYLRDGEVVIFAGGTGRPFFTTDTASTLFASEIDADAILMGKNNVDGVYDSDPKFNKDAKKFPIITYDELIEKELKVIDQTAASMAKENNIDILIFDINEEDSLIRAIENKIPNTIITREREK", "aa_hexdigest": "bc89fd2a62eaff8825ac71e16e191684", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P6F8", "query_cov": 0.96045197740113, "subject_cov": 1.0, "identity": 0.835, "score": 286.0, "evalue": 4.53e-97, "valid": false, "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_A0A3B0P6F8", "kegg_orthology_id": "K09903" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P6F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "product": "UMP kinase" }, "genes": [], "id": "AHCKFKLKGB_998", "locus": "GCA013367975_00997" }, { "type": "cds", "sequence": "contig_1", "start": 580740, "stop": 581291, "strand": "+", "frame": 3, "gene": "frr", "product": "Ribosome recycling factor (Ribosome releasingfactor)(RRF)", "db_xrefs": [ "COG:COG0233", "COG:J", "RefSeq:WP_044329746.1", "SO:0001217", "UniParc:UPI0005C83D5E", "UniRef:UniRef100_UPI0005C83D5E", "UniRef:UniRef50_B3PLW1", "UniRef:UniRef90_D1J8P9" ], "nt": "ATGGAATTAGACAGTTATCTTTTAGAATTAGATGAAGAAATCAGCAAATCAATTACTAACTATGAAAATCAATTAATGAAAGTTGTTATAGGTAGAGCCAACCCTGTTTTAATCAGTAAAATTAAGTTAGAATATTATGGTTCACCAATTTCAGTAGAAGAAATAGCATCCATAACTACATCGGGGCCATTACAATTAATTGTTAAGCCTTATGATATGAGTGTATTAAAAAATATTGAAAAAGCAATCATGGACAACAAGATAAACGTTTCAATAATAAATGAAGGACACCAATTGAGATTGCAATATCCTCAAATGACCACTGAAAAAAGAGTAGAAATGACAAAGCAACTTGCTTCAATAACCGAACAAGCAAGAATAGGCGTAAGACAAGCAAGACAAGATATCAATAAAAAAATAAAAGCAGATAGCGACCTTTCAGAAGACATTCAAAAACATTATCTAGATAATATTCAAAAAGAAGTTGATAAATCAATAGAAAAAATTAATGAAATGTCAAAAACAAAAGAAAAAGAACTTTTGACAATATAA", "aa": "MELDSYLLELDEEISKSITNYENQLMKVVIGRANPVLISKIKLEYYGSPISVEEIASITTSGPLQLIVKPYDMSVLKNIEKAIMDNKINVSIINEGHQLRLQYPQMTTEKRVEMTKQLASITEQARIGVRQARQDINKKIKADSDLSEDIQKHYLDNIQKEVDKSIEKINEMSKTKEKELLTI", "aa_hexdigest": "55a096ccefd76ab8d64c2c5e0c6bd8a0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0005C83D5E", "ncbi_nrp_id": "WP_044329746.1", "uniref100_id": "UniRef100_UPI0005C83D5E", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C83D5E", "RefSeq:WP_044329746.1", "UniRef:UniRef100_UPI0005C83D5E" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C83D5E", "uniref90_id": "UniRef90_D1J8P9" }, "psc": { "uniref90_id": "UniRef90_D1J8P9", "gene": "Frr", "product": "Ribosome recycling factor (Ribosome releasingfactor)(RRF)", "uniref50_id": "UniRef50_B3PLW1", "cog_id": "COG0233", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_B3PLW1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLW1" ], "product": "Ribosome recycling factor" }, "genes": [ "frr" ], "id": "AHCKFKLKGB_999", "locus": "GCA013367975_00998" }, { "type": "cds", "sequence": "contig_1", "start": 581349, "stop": 581555, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACTTAAAAATTAGAGCAAAATCTGCGATTATATTATTCAGTATATTGATTCCACTACTTATTTTAACTTATTTTGTTAAAAGTTTAGGAGTGGCAATTTATATGTTTTTTAGTGCATGGGCTTTGTATGAAGTTATTTCACACAACCAAAATAAAAGGCCTATTAATTTAGTTATATCCTTACTATCAATATTAATTTGA", "aa": "MKNLKIRAKSAIILFSILIPLLILTYFVKSLGVAIYMFFSAWALYEVISHNQNKRPINLVISLLSILI", "aa_hexdigest": "96318dc3298dd3aa8426d692445da4e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7778.480399999998, "isoelectric_point": 10.214580345153806 }, "id": "AHCKFKLKGB_1000", "locus": "GCA013367975_00999" }, { "type": "cds", "sequence": "contig_1", "start": 581604, "stop": 581792, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAGCAATGGACATTGGGTCTATGGCCTTGATAGAGATACATTGATTTCAATAATAAAAAATACAATATTCTTTAAATCAAATGCCTGGTCTGGTGCTCCGGGGTTGGGCTTAATATTGGTTTTCTTAATAACAACATTCTTACTCATTGTTGATGTAAAGAGTCAAAAAAATGCTAAGGATTGA", "aa": "MYSNGHWVYGLDRDTLISIIKNTIFFKSNAWSGAPGLGLILVFLITTFLLIVDVKSQKNAKD", "aa_hexdigest": "dccc6a5c1ebc40f1e61aba7bad1a5279", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6945.043900000001, "isoelectric_point": 9.224281501770019 }, "id": "AHCKFKLKGB_1001", "locus": "GCA013367975_01000" }, { "type": "cds", "sequence": "contig_1", "start": 582060, "stop": 582302, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGATTAATTTTGGAAACTCTGATTCAATAAAAATATTAATAAATCAAAAGCAAATTATTACGGCAGTTTTTGTTCTTCCTGCAATTTCTATTATTGGTGACTTGGTATTTTCAGGAATAAAAAGAGCTCAAAATATTAAGGATTTTTCTGAATTAATAGCGGGTCATGGAGGAATTATGGATAGATTTGATTCGCTATCGTTTAATACGGTATTTTTAACTTTTATATTAATGATTTAA", "aa": "MGINFGNSDSIKILINQKQIITAVFVLPAISIIGDLVFSGIKRAQNIKDFSELIAGHGGIMDRFDSLSFNTVFLTFILMI", "aa_hexdigest": "3abe1bc2c0a1005faea91ebe37609cbc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8790.275099999995, "isoelectric_point": 6.520194053649903 }, "id": "AHCKFKLKGB_1002", "locus": "GCA013367975_01001" }, { "type": "cds", "sequence": "contig_1", "start": 582405, "stop": 582926, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATAAGACTTTTGTGCAATTATGCAAAGAAATAAAATTTGAATTTGATGAACAACAATTTTATGGTGCAAAAGTTTTGAGTGTTGAATCAAACTTAAATGAATGGAAATTTAAGATTGCCTTTGCAAACCATATTGACATTGAACAATACAAACAATTTAGAGAAAAATTAGTTAAAAGATTTGGCAAGGTTGAATTAATCTTGGCTATTGAGACAATTATAAGAAATAAACAAATTATTGTTGATTATTTAAATTATCTAATAGAATGTAACCCCAAGAAATATTGGCCTTTTGTGAAATATAATTATTCATGTATAACTACTTCAACAATTGAAATAGACGAAAAAACATTAACACTTTCCTTGCTAACAAATGAAATTTATGAAGTTTTTAAAAGTAATAAAGATCTTTTTAAAGAAGCGCTTGCTTCAATAGGATTTGATTTTTATAATGTAGTTTTTAATTTGGGATTAAACAATAAAAAAAGCGTTGCAAGCAAAAAACTAGACGGTTGA", "aa": "MLDKTFVQLCKEIKFEFDEQQFYGAKVLSVESNLNEWKFKIAFANHIDIEQYKQFREKLVKRFGKVELILAIETIIRNKQIIVDYLNYLIECNPKKYWPFVKYNYSCITTSTIEIDEKTLTLSLLTNEIYEVFKSNKDLFKEALASIGFDFYNVVFNLGLNNKKSVASKKLDG", "aa_hexdigest": "59fb7d8c6cbab26d17a4c7ce12cfe412", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20402.415500000006, "isoelectric_point": 8.278788185119634 }, "id": "AHCKFKLKGB_1003", "locus": "GCA013367975_01002" }, { "type": "cds", "sequence": "contig_1", "start": 583029, "stop": 584099, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGGACTTAACTACGGCAAACGAAACATTTGAAGAAATAAAAATAACCACAAAAGGAGAAGTTTTTTTTATTGAAAGTAAAGTGCTAAAAAACAAAAAAATTCTATATACGCTAAAGATTTCAGATTATAAAGAAGCTATTCTTATTAAAAAATTTTATGATTCTGAAAACGAAATACCCGATATAAAGGTAGGGGACACGATTATTGTTACTGGAAAATTGAGTGATGATAATTTTACAAAATCAAAAGTTTTAATAGCAATGGGCAAGGAATGCTTCATAAAGACAGAACCATTACAAAAACTTATTGAAGATAACGAAGACAATAAAAGAGTTGAATTAGCCGCAAGATCTAATATGAGTGCTCAAGATGGAATTTCAACACCATCTGATTATATTGATGCTGCTAAATTTTATGGTCATAAGGCTATAGCATTGACCGACCTAGATGATGCGCAAGGCTTTCCTGAATTTTATAATTATGCCAAAAAGCAAAAGGATGTTAAGCCAATATACGGCGCAACATTAAGCACCGTTGATTCAGATAAAAAATTCTTCTACAATTTTAAAAATTTCAACTTAAAAGAAGAAGAATATGTTGTGTTTGACCTTGAAACAACAGGATTGAGCCCAAGATTGGATGACATTATTGAATTTGGTGCCACTATTATAAAAAATAATACTATTACTCAAAAAATTCAATTCTTTTTACAAACAAAACGTGAGCTATCAGCCTTTACTGTTGAAAGCAGTCACATTAATAATGAAATGTTAAAGAATGGTTATGAACAAGAAGAAGGCATTAAAAAAATTTATGAAATATTAAAGGATAAAATAGCAGTTGCTCACAATGCAAACTTCGATATAAATTTTTGCAAAGAAAAATTTGGCAAACTCGGCCTTGATAATTCTAAAATTTTTGGCCTTGATACATTGGCCTTATCTTATTTTTTAAACCCTGATGAATTTCGTCATTCTTTGGGTTATGTTGCTAAAAGATTCAATATTGTTTATGATACAACTGTTGCCCACCGTGGTGATTACGACGCTAATGTTTTGGCAAAGATTTGA", "aa": "MDLTTANETFEEIKITTKGEVFFIESKVLKNKKILYTLKISDYKEAILIKKFYDSENEIPDIKVGDTIIVTGKLSDDNFTKSKVLIAMGKECFIKTEPLQKLIEDNEDNKRVELAARSNMSAQDGISTPSDYIDAAKFYGHKAIALTDLDDAQGFPEFYNYAKKQKDVKPIYGATLSTVDSDKKFFYNFKNFNLKEEEYVVFDLETTGLSPRLDDIIEFGATIIKNNTITQKIQFFLQTKRELSAFTVESSHINNEMLKNGYEQEEGIKKIYEILKDKIAVAHNANFDINFCKEKFGKLGLDNSKIFGLDTLALSYFLNPDEFRHSLGYVAKRFNIVYDTTVAHRGDYDANVLAKI", "aa_hexdigest": "97bc6bb9f4a8d1cfdabe0c148f87efff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 143, "aa_cov": 0.3960674157303371, "hmm_cov": 0.8321678321678322, "evalue": 1.5020465991039124e-08, "score": 30.07735824584961, "start": 201, "stop": 341 }, { "id": "PF00929.31", "name": "RNase_T", "length": 165, "aa_cov": 0.4410112359550562, "hmm_cov": 0.9878787878787879, "evalue": 3.140269950649337e-32, "score": 107.68050384521484, "start": 200, "stop": 356 } ], "seq_stats": { "molecular_weight": 40683.776699999995, "isoelectric_point": 5.401034355163574 }, "id": "AHCKFKLKGB_1004", "locus": "GCA013367975_01003" }, { "type": "cds", "sequence": "contig_1", "start": 584106, "stop": 584369, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGACAAACTTGCAAAAGATTACAATATCCTAAACTCACAGGATTTAAACAATGTCGATAGCCAAAAAATACTTTCTCATAAATTTACATACGAAACAAGAATATTGGTAAAAAATCAAGCCGGACTTAAAAAATTATTCAAAATTATTTCGCGTTCTTTAACTTCTCAATTTAAAAAAAATCCACAAATTTACTTTGATCAATTGGGCAATGATGAAGATTTGTTTTTTGGATCAGCAACAAATCAATCATATGTTTGA", "aa": "MIDKLAKDYNILNSQDLNNVDSQKILSHKFTYETRILVKNQAGLKKLFKIISRSLTSQFKKNPQIYFDQLGNDEDLFFGSATNQSYV", "aa_hexdigest": "7f2aa703e437c38f42ef82ce7b1a8918", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10093.354599999991, "isoelectric_point": 9.222669792175292 }, "id": "AHCKFKLKGB_1005", "locus": "GCA013367975_01004" }, { "type": "cds", "sequence": "contig_1", "start": 584400, "stop": 584978, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAAATTCACTAAAATATTATGATTATATTGAATTACCCCCTATATCATCATTTCAATATATGATTGATAGTAACCACGTTACATTAGATGAATTAAAATTTTTGTATAAAGATTTAATTTCAAAAGCAAAAAGTTTAAACAAAATGTGCGTTGCTGTTTCTGATTGTAGATATATTTATAATTATCAAAGATTAATTCATGAAATTTATATTGAGGCTCCAACTTTGGGGGGTGGGCTTCATTGGCTTAAGAAGAGACCATCTCATCCAGAATTTAGATATTTAACAACAAGAGAGATGTTAAAAGAATTTGCTTTTTTGAATGATAGTTATTTAATTAATGAAATAGTAATTGAAAATCCAAACAAAATTGCTAATGAAATTTCATCTAATATTCAAGTTATTAAAGATAAATTGTATGTTCCAACTTTTGACAATTCGCCTCAAAAATTAAGAGAAGTTGTTTATAGTACTTTAAAAGAAAGATACGGAGATAATCCTGATCCCATCATAGTTAAAAGAATTGAAAAGGAATTAAACCCAATTATAAAGTATGGCTATTCTGTTATTTATTGA", "aa": "MLNSLKYYDYIELPPISSFQYMIDSNHVTLDELKFLYKDLISKAKSLNKMCVAVSDCRYIYNYQRLIHEIYIEAPTLGGGLHWLKKRPSHPEFRYLTTREMLKEFAFLNDSYLINEIVIENPNKIANEISSNIQVIKDKLYVPTFDNSPQKLREVVYSTLKERYGDNPDPIIVKRIEKELNPIIKYGYSVIY", "aa_hexdigest": "7a1f7804442ee722b926f55f73ac837f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22688.965100000016, "isoelectric_point": 7.687269020080567 }, "id": "AHCKFKLKGB_1006", "locus": "GCA013367975_01005" }, { "type": "cds", "sequence": "contig_1", "start": 585171, "stop": 585449, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023B0427D", "PFAM:PF07733.18" ], "nt": "TTGCCGGACAAGCATTGTCCAAATTGCAACTCAATATTATCAAAAGATGGGCACAATATTCCGTTTGAAACATTTTTAGGATTTGAAGCGGATAAGGTTCCTGATATCGATTTAAATTTTAGTGGTGAATATCAACCAACAATTCACAATTATGTTAAGGAATTGTTTGGTGAAGCACATACATATAGAGCCGGTACAATAGCTACCGTAGCAGAAAAAACAGCATATGGATTTTGTAAAAAATATGAAGAAGAAAAGGGGGGAAATTATCAACCTTGA", "aa": "MPDKHCPNCNSILSKDGHNIPFETFLGFEADKVPDIDLNFSGEYQPTIHNYVKELFGEAHTYRAGTIATVAEKTAYGFCKKYEEEKGGNYQP", "aa_hexdigest": "4f10f97a4ab46f0843b13e6749ea7beb", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00311D31FE", "query_cov": 0.8695652173913043, "subject_cov": 0.9523809523809523, "identity": 0.688, "score": 122.0, "evalue": 7.9e-35, "valid": false, "uniref50_id": "UniRef50_UPI0023B0427D" }, "pscc": { "uniref50_id": "UniRef50_UPI0023B0427D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023B0427D" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 59, "aa_cov": 0.6413043478260869, "hmm_cov": 1.0, "evalue": 1.186791190738871e-19, "score": 66.25160217285156, "start": 18, "stop": 76 } ], "seq_stats": { "molecular_weight": 10378.415999999994, "isoelectric_point": 5.227789115905761 }, "id": "AHCKFKLKGB_1007", "locus": "GCA013367975_01006" }, { "type": "cds", "sequence": "contig_1", "start": 585456, "stop": 585605, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07733.18" ], "nt": "TTGTTCTTAGATTTTTTAGCATCTAAAACACAAGGGGTAAAGAGGACAACTGGACAACACCCGGGAGGAATTATTATTATTCCTAAAGAATTCGATGTAGAGGATTTTACGCCTGTAAACTATCCTGCAAACGACACAGAAAGCCCTTGA", "aa": "MFLDFLASKTQGVKRTTGQHPGGIIIIPKEFDVEDFTPVNYPANDTESP", "aa_hexdigest": "013ec568ffe5429f053f3ee9f906ae61", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 40, "aa_cov": 0.8163265306122449, "hmm_cov": 0.95, "evalue": 2.029927407355366e-09, "score": 32.711971282958984, "start": 6, "stop": 45 } ], "seq_stats": { "molecular_weight": 5423.026500000003, "isoelectric_point": 4.646496772766113 }, "id": "AHCKFKLKGB_1008", "locus": "GCA013367975_01007" }, { "type": "cds", "sequence": "contig_1", "start": 585663, "stop": 585941, "strand": "+", "frame": 3, "gene": null, "product": "DNA polymerase III polC-type", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PG80" ], "nt": "TTGGGTCATGATGATCCCACTGTTATTAGAATGCTTGAAACATTGACAAACACAAATGCAAAGGAAATACCAAAATTTGATGCAGAAGTTATGAAACTTTTCTATACAACTGAATCATTGAAAATTAGTCCTTCAGACATTGGAGGCGAAACAACTGGGGCTTACGGGTTACCTGAATTTGGAACAAATTTTGTAAGAAAAATGCTAAAAAAGGCTCAGCCAAGATCATTTAATGATTTAATTCTTCTTTCGGGGTTGAGTCATGGTACGGATGTTTGA", "aa": "MGHDDPTVIRMLETLTNTNAKEIPKFDAEVMKLFYTTESLKISPSDIGGETTGAYGLPEFGTNFVRKMLKKAQPRSFNDLILLSGLSHGTDV", "aa_hexdigest": "23a80e91fd97067934672b2143b848b8", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FBH3", "query_cov": 1.0, "subject_cov": 0.9019607843137255, "identity": 0.62, "score": 117.0, "evalue": 2.58e-32, "valid": false, "product": "DNA polymerase III alpha subunit finger domain-containing protein", "uniref50_id": "UniRef50_A0A3B0PG80" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PG80" ], "product": "DNA polymerase III polC-type" }, "genes": [], "id": "AHCKFKLKGB_1009", "locus": "GCA013367975_01008" }, { "type": "cds", "sequence": "contig_1", "start": 586248, "stop": 586493, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTTTATGCTTCATTTTATACAAACAGGCCAGATGCCATCGATATACAAATAATGAGTAGTGGCATAGCAAGCGTTCACCAAAAACTTCTTGAATTAAAAAATCGCCAAAATTCAAATGGCGAAAAATTATCTGTAAAGGAAGAAGCATTAATTCCTGTTTTAGAAATTACTCAAGAATTATATGCAAGGGGATTTAAAATTAAAAATGTTGATTTAAAACTTTCTTTGGCTGATAAATGA", "aa": "MPFYASFYTNRPDAIDIQIMSSGIASVHQKLLELKNRQNSNGEKLSVKEEALIPVLEITQELYARGFKIKNVDLKLSLADK", "aa_hexdigest": "86548e4a860b8481320c3e8649482ae7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9181.505499999988, "isoelectric_point": 7.985650444030763 }, "id": "AHCKFKLKGB_1010", "locus": "GCA013367975_01009" }, { "type": "cds", "sequence": "contig_1", "start": 586548, "stop": 586718, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14579.12" ], "nt": "TTGGGAATTACTGTTGCTCAAACAATTGTATCTGCAAGAAAAGAAGGTTTTTTCCTTTCAATTGAAGACCTAGCTTCGAGAACAAAAGTCAATTCAAGAATTATTTCAGAATTAAAAAAAATAGGTGTTTTAGATTGCTTAGATGATACCAATCAAGTAAGTTTGTTTTAA", "aa": "MGITVAQTIVSARKEGFFLSIEDLASRTKVNSRIISELKKIGVLDCLDDTNQVSLF", "aa_hexdigest": "971014d95775f5861e4a473bea170835", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14579.12", "name": "HHH_6", "length": 53, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 4.141603860121459e-10, "score": 35.10380935668945, "start": 2, "stop": 50 } ], "seq_stats": { "molecular_weight": 6172.090299999998, "isoelectric_point": 6.079237174987792 }, "id": "AHCKFKLKGB_1011", "locus": "GCA013367975_01010" }, { "type": "cds", "sequence": "contig_1", "start": 587381, "stop": 587668, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGAAATTGGAATTAGAAAGGTTAATGCTTCAGTGTTAAATACCTTAAATTTAAAAACTAACAATAAAACAATAATAATTTGTATTGCAGCAATTGCCAATGCTATTGGAAACTCAACACCTCATCTTGATAAATTTTTTGTTGATTTTATAATTCAACCATCTATGCCTAAAATTAAAAACATTATAAAAGAAGGAATGACAATTAAAAATAAACCCGTTAGAGCTATTTTTCATGAACGTGACAATTCTCTTTTTGGCTTTCCTGATTCTACAACATAA", "aa": "MAKEIGIRKVNASVLNTLNLKTNNKTIIICIAAIANAIGNSTPHLDKFFVDFIIQPSMPKIKNIIKEGMTIKNKPVRAIFHERDNSLFGFPDSTT", "aa_hexdigest": "68b5c6f1d8ce42c76e84c1234f52ebb4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10553.310799999988, "isoelectric_point": 9.843113517761228 }, "id": "AHCKFKLKGB_1012", "locus": "GCA013367975_01011" }, { "type": "cds", "sequence": "contig_1", "start": 587899, "stop": 589155, "strand": "+", "frame": 1, "gene": "glyA", "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "COG:COG0112", "COG:E", "EC:2.1.2.1", "KEGG:K00600", "RefSeq:WP_044420793.1", "SO:0001217", "UniParc:UPI0005C81B46", "UniRef:UniRef100_UPI0005C81B46", "UniRef:UniRef50_P56089", "UniRef:UniRef90_D1J8Q3" ], "nt": "ATGTATAAAAAAATAAGTTTAGATGATAAAGAAATCGAAGAATTAATTAACCTAGAAAATCAACGTTTAGAAGACCATGTAGAACTAATAGCATCTGAAAATTATGTTTCAGAAGATGTTATGAAGGCAAATGGTTCTTGTTTAACTAATAAATATGCAGAAGGCTATCCTAATAAAAGATATTATGGTGGTTGCGAATATGTTGATAAAATAGAAGAAATTGCACAAGAAAGAGCAAAAAAATTATTCAATGTAAAATATGCTAATGTTCAACCATATTCAGGAAGTGTTGCAAATGCTGCTGTATACATGGCAATGGTTGATCCTGGAGATAAAGTCTTAGGATTAGATTTAAATTCAGGAGGACATTTATCACACGGATATAAAATTTCATTTAGTGGTAAATTTTACGAAGCACATACCTATTCTGTTAACGATCAAGGTTTATTGGATTACGACAAGATATTGGAAATTGCAAAAGAAGTAAAACCAAAAATGATAATTTGTGGATATTCTGCATATTCTCAAATTGTTGACTTTGCTAAATTTAGAAAAATTTGTGATGAAGTAGGCGCAAAATTATTTGCCGATATTTCTCATATATCAGGATTAATAATAGCAGGATTACACCCATCACCTTCGGGTTATGCTGATGTTATAATGACTACAACACATAAGACATTGCGTGGCACTCGTGGAGCAATAATTTTAACTAATGACGAAGAAATTTTTAAAAGAGTTAACAGTGCGGTTTTTCCTGGCGTTCAAGGAGGACCTTTGTTGCACCAAATAGCGGCAAAGGCAGTAAGTTTTAGAGAAGCATTACAACCAGAGTTTATTGATTATCAAAAGCAACTATTGTTGAACTCAAAAATTATGTGTCAAACATTTTTGAACAAGGGAGTAAAAATTATCTCTGGCCTTACTCAAAATCATTTATTTATGATTGATGTAAAAACTAGTTATGGAATAACAGGAAAATATGCAACAGAAGTTTTGGGTAGAGTTAATATAACCGTGAATAAAAACACTATTCCAAACGATAGTGAAAAACCAACCGTTGCTAGTGGAATAAGAATAGGATCTGCGGCAATGACATCAAGAGGATTAAAGGAAGATGAGTTCATCATTTTAGCCAATTTAATTGATAAAGTTTTAAGAGATCCAGAAAACGAAAACTTATTATATGTAGTAAAGAAAGAAATTGCAAAATTAACCAATTCATTTCCAATTAAACGTTCTCATTTAGTTCGTTAG", "aa": "MYKKISLDDKEIEELINLENQRLEDHVELIASENYVSEDVMKANGSCLTNKYAEGYPNKRYYGGCEYVDKIEEIAQERAKKLFNVKYANVQPYSGSVANAAVYMAMVDPGDKVLGLDLNSGGHLSHGYKISFSGKFYEAHTYSVNDQGLLDYDKILEIAKEVKPKMIICGYSAYSQIVDFAKFRKICDEVGAKLFADISHISGLIIAGLHPSPSGYADVIMTTTHKTLRGTRGAIILTNDEEIFKRVNSAVFPGVQGGPLLHQIAAKAVSFREALQPEFIDYQKQLLLNSKIMCQTFLNKGVKIISGLTQNHLFMIDVKTSYGITGKYATEVLGRVNITVNKNTIPNDSEKPTVASGIRIGSAAMTSRGLKEDEFIILANLIDKVLRDPENENLLYVVKKEIAKLTNSFPIKRSHLVR", "aa_hexdigest": "b8c5082caf53703babeccbbbd683622a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0005C81B46", "ncbi_nrp_id": "WP_044420793.1", "uniref100_id": "UniRef100_UPI0005C81B46", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C81B46", "RefSeq:WP_044420793.1", "UniRef:UniRef100_UPI0005C81B46" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C81B46", "uniref90_id": "UniRef90_D1J8Q3" }, "psc": { "uniref90_id": "UniRef90_D1J8Q3", "gene": "GlyA", "product": "Serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_P56089", "cog_id": "COG0112", "cog_category": "E", "kegg_orthology_id": "K00600" }, "pscc": { "uniref50_id": "UniRef50_P56089", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P56089" ], "product": "Serine hydroxymethyltransferase" }, "genes": [ "glyA" ], "id": "AHCKFKLKGB_1013", "locus": "GCA013367975_01012" }, { "type": "cds", "sequence": "contig_1", "start": 589166, "stop": 589462, "strand": "+", "frame": 2, "gene": null, "product": "Asp23/Gls24 family envelope stress response protein", "db_xrefs": [ "RefSeq:WP_012855740.1", "SO:0001217", "UniParc:UPI0001BC24B5", "UniRef:UniRef100_D1J8Q4", "UniRef:UniRef50_A0A3G8LGV6", "UniRef:UniRef90_D1J8Q4" ], "nt": "ATGATATTAGCGGAAATAAAAAATTTAATTAAATCTTCAATAAGTATTATTCCAGGAATAGTTGATTTTGTTGAATTAACTTCAACCAATAATATAAATATTGAAAATGATGGTTTAATCATTGAGGAAGATCCACAAAATAAAAACATTATAAATATAACAATTGGTTTGAGAATAATGAGCAATTTAAGTGCTAAAAATATCGTTGAAGGAATATATGAAATCATTCCATATAACTTAACTTCTTACGATATAAAATTAGGAACATTAACAATATATATTAAAGGAACTAAATAA", "aa": "MILAEIKNLIKSSISIIPGIVDFVELTSTNNINIENDGLIIEEDPQNKNIINITIGLRIMSNLSAKNIVEGIYEIIPYNLTSYDIKLGTLTIYIKGTK", "aa_hexdigest": "7f63b54d2cc3e32b5b5d28cdfbd465c3", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001BC24B5", "ncbi_nrp_id": "WP_012855740.1", "uniref100_id": "UniRef100_D1J8Q4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC24B5", "RefSeq:WP_012855740.1", "UniRef:UniRef100_D1J8Q4" ] }, "ips": { "uniref100_id": "UniRef100_D1J8Q4", "uniref90_id": "UniRef90_D1J8Q4" }, "psc": { "uniref90_id": "UniRef90_D1J8Q4", "uniref50_id": "UniRef50_A0A3G8LGV6" }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LGV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGV6" ], "product": "Asp23/Gls24 family envelope stress response protein" }, "genes": [], "id": "AHCKFKLKGB_1014", "locus": "GCA013367975_01013" }, { "type": "cds", "sequence": "contig_1", "start": 589591, "stop": 591090, "strand": "+", "frame": 1, "gene": "fakA", "product": "DhaL domain-containing protein", "db_xrefs": [ "COG:COG1461", "COG:I", "SO:0001217", "UniRef:UniRef50_N9TTI7", "UniRef:UniRef90_D1J8Q5" ], "nt": "ATGTCATCTACAATTTCTTCTGCATGTAATTCTGAATGCTTAGAAGACGATTGCAGTATTGAATCAGCTTCGAGAATTGTTGCTCAAAATATGTTAATGTCTGCCAGAGGAAATTCTGGTGTAATTTTAAGTCAAATTTTTAAAGGTTTTTCTAATGGGTTTGCATCTAAAAAGTCTGCTAATGCTTTTGATTTAGTTAATGCTTTCGAAATGGCTGCTAAATCAGCTTATGGTGCAGTTTTAAAACCTGTTGAAGGAACTATTTTAACCGTTATTAGAGAAGTTTCTGAAGGTTTAAAAAACCAAGTTGTGGTTGATACTACTATTGAAGAAGTTTTTGAAATTGCTGTTAAATTAGCAAGAAAAAGTTGTGATGGTACTCCTAAATTATTAAAAATATTAAAAGAAGTAGGTGTTACTGATTCAGGTGGTGAAGGTTTATATGCAATATTGCAGGGAATGAGTGCATACTTTAGTGGTAATAAAGTAGAACCTATTGTTGCAGAAGAGTCGGTTGAAAAATTTATTTCTGATGAAGAGGTATATGAAGGCGAATTTGGATATTGCACCGAATTTATTTTGGAATTATATAAACCAAAAAAATTCGACAAAGATTATTTAGTTCGTAAACTAGAAAAAATTGGTAATTCAATGGTTGTGGTAAATGATGACAAAATTTTAAAAGTTCATATTCATACCCCTAAGCCCGGTGATGTTTTAAATAGCGTAAATTCTTTGGGACAATTTGCAAAAATTAAAATAGATAACATGACTTTACAAGCAAACAATAGTAAGAAAGCTGCAAAGAAAAAACAAACCGAAGCGCCAAACAATGTTGAATTAAAAAATAGCGCCATTGTTTCGTGCAATACAGGCCAAGGAATCGTAACCTTAATGAAAGAAAACGGTGTTTCATATGTTGTTGATGGCGGTCAAACAAATAATCCTTCAATTCAAGATATTGTTAATGCAATAAATGCGGTCAATGCAAAAACGGTATTTGTATTGCCAAATAACTCAAATATAATTTTATCTGCTCAACAAGCCGGCACAATTATTAGAGATAAGAATGTAATTATCATTCCAACTAGGAGTCAAGTTCAAGGGTTAAATGTTGCATTGAATTATAGTTCTGAAAATAGTGATGAAGAAAATCACGAATTAATGAGCAATAGTTTAAAACATATAAGATATGGAGAAATTGCTGCCGCTATAAAAGATGCTAAATTAAACGGAATAAAGGTTAAAGCATCCGATTATATGGTTATTGTAGAAGGAAAATTACAAAAAACATCAAAGAGTCTATTCAACGCAGCACAATTATTAGTATCAGAATTAGTTTCGGATGATAGTCAAGTAGTAACAATAATATATGGCCAAGATGTTTCGGAAAATGATGCGCTAGAATTACAAAATTATATTGAAACAACATACGACCTTGTTGTAGAAATTTATCAAGGTGACCAAGGAATTTATCCATATTTAATCTCAGTCGAATAG", "aa": "MSSTISSACNSECLEDDCSIESASRIVAQNMLMSARGNSGVILSQIFKGFSNGFASKKSANAFDLVNAFEMAAKSAYGAVLKPVEGTILTVIREVSEGLKNQVVVDTTIEEVFEIAVKLARKSCDGTPKLLKILKEVGVTDSGGEGLYAILQGMSAYFSGNKVEPIVAEESVEKFISDEEVYEGEFGYCTEFILELYKPKKFDKDYLVRKLEKIGNSMVVVNDDKILKVHIHTPKPGDVLNSVNSLGQFAKIKIDNMTLQANNSKKAAKKKQTEAPNNVELKNSAIVSCNTGQGIVTLMKENGVSYVVDGGQTNNPSIQDIVNAINAVNAKTVFVLPNNSNIILSAQQAGTIIRDKNVIIIPTRSQVQGLNVALNYSSENSDEENHELMSNSLKHIRYGEIAAAIKDAKLNGIKVKASDYMVIVEGKLQKTSKSLFNAAQLLVSELVSDDSQVVTIIYGQDVSENDALELQNYIETTYDLVVEIYQGDQGIYPYLISVE", "aa_hexdigest": "8ea5ec3548aea39c012b1f78dd498cd2", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J8Q5", "query_cov": 1.0, "subject_cov": 0.9206642066420664, "identity": 0.996, "score": 929.0, "evalue": 0.0, "valid": true, "gene": "FakA", "product": "DhaL domain-containing protein", "uniref50_id": "UniRef50_N9TTI7", "cog_id": "COG1461", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_N9TTI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TTI7" ], "product": "DhaL domain-containing protein" }, "genes": [ "dhaL", "fakA" ], "id": "AHCKFKLKGB_1015", "locus": "GCA013367975_01014" }, { "type": "cds", "sequence": "contig_1", "start": 591100, "stop": 591579, "strand": "+", "frame": 1, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0008610", "GO:0043811", "SO:0001217", "UniRef:UniRef50_D1J8Q6", "UniRef:UniRef90_D1J8Q6" ], "nt": "ATGAAAAAAATAGTATTTGATTTATTGAATAATGATAATGGCGAAGAACAAGCGATTTTGGGTGCAAAAAGATTTTTGCAAGAAAATTCAGATTATTTTTTAATTTTGGTTGGTCATGAACAACTTATCAAGAAACATTTATCAGATTACATTGATAGGATTGCCATAATTAATAATGAAACAATTGTACACATTGAATCTAGTCCAAGAGACGTATTGAGAACTCAAAATTCAATGCTAGAGGCTTTTAAACAACTCAACGAATCAAATGCAGATGGAATATTGAGTAGTGGAGATAGCGGCTCGTTAATAACATTATCATCATTGAAAGTTAAAAGGCTTCTTGGGATTGAAAGACCAGCATTCATGCCAATTCTACCAACAACAACGGATAAAAAAATGTTATTAATAGATGCGGGAGCAAACATTGAAATCAAACCAGAATATTTAAGCCAATGGGCTATGATAGCAAGTGAATGA", "aa": "MKKIVFDLLNNDNGEEQAILGAKRFLQENSDYFLILVGHEQLIKKHLSDYIDRIAIINNETIVHIESSPRDVLRTQNSMLEAFKQLNESNADGILSSGDSGSLITLSSLKVKRLLGIERPAFMPILPTTTDKKMLLIDAGANIEIKPEYLSQWAMIASE", "aa_hexdigest": "922e9b1b0cb019f893741adff7fa6e4f", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "plsX" ], "truncated": "3-prime", "pseudogene": { "start": 591100, "stop": 591882, "observations": { "mutation": [ 591577, 591865 ], "stop": [ 591577, 591865 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8Q6", "query_cov": 1.0, "subject_cov": 0.4746268656716418, "identity": 0.9940000000000001, "score": 304.0, "evalue": 5.72e-102, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MKKIVFDLLNNDNGEEQAILGAKRFLQENSDYFLILVGHEQLIKKHLSDYIDRIAIINNETIVHIESSPRDVLRTQNSMLEAFKQLNESNADGILSSGDSGSLITLSSLKVKRLLGIERPAFMPILPTTTNKKMLLIDAGANIEIKPEYLSQWAMIASEWYSILFGSKKPIVGQLNVGVEEYKGSEITKNAKNILKEVSKSANFVFNGYVEATNAINGDIDILVVDGYGGNVFLKTLEGTFIAFSKLLKNILLKNWKTKLAALILKRHLREFKQKFDYRNTGGAFIIGLEKIVVKAHGGSDELAFYNALNQIKIGIENNISTILNKKFKEADNEE" }, "paralog": false, "identity": 0.9884615384615385, "score": 494.0, "evalue": 3.13e-179, "description": "Internal stop codon at 591577, 591865. Nonsense mutation around 591577, 591865.", "psc": { "uniref90_id": "UniRef90_D1J8Q6", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_D1J8Q6", "cog_id": "COG0416", "cog_category": "I", "go_ids": [ "GO:0008610", "GO:0043811" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8Q6", "EC:2.3.1.274", "UniRef:UniRef50_D1J8Q6", "COG:COG0416", "COG:I", "GO:0008610", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8Q6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8Q6" ], "product": "Phosphate acyltransferase" } }, "id": "AHCKFKLKGB_1016", "locus": "GCA013367975_01015" }, { "type": "cds", "sequence": "contig_1", "start": 591589, "stop": 591867, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCGGAAGCAAAAAGCCAATAGTAGGACAATTAAACGTAGGCGTCGAAGAATACAAAGGTTCTGAAATAACAAAGAATGCTAAAAATATATTAAAAGAAGTTTCTAAATCTGCTAATTTTGTTTTTAATGGTTATGTTGAAGCAACAAATGCCATTAATGGAGATATAGATATATTAGTGGTTGATGGATATGGCGGAAATGTGTTTCTAAAAACATTAGAAGGTACCTTTATTGCTTTTTCAAAATTATTGAAAAATATTTTATTAAAAAATTGA", "aa": "MFGSKKPIVGQLNVGVEEYKGSEITKNAKNILKEVSKSANFVFNGYVEATNAINGDIDILVVDGYGGNVFLKTLEGTFIAFSKLLKNILLKN", "aa_hexdigest": "59450b628a35b04801e5ffb8ffc405e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9999.448399999992, "isoelectric_point": 8.892075920104983 }, "id": "AHCKFKLKGB_1017", "locus": "GCA013367975_01016" }, { "type": "cds", "sequence": "contig_1", "start": 592097, "stop": 592630, "strand": "+", "frame": 2, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "COG:COG0571", "COG:K", "EC:3.1.26.3", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396", "SO:0001217", "UniRef:UniRef50_D1J8Q7", "UniRef:UniRef90_D1J8Q7" ], "nt": "ATGAAGAATAATTTTGAAGAAAATTTACAAAATGCATTGCTAAAATTTGGTATAAATTTTCAAGAATTAAATCAACAAAAGAGACATTATTTTTATAATGCTTTTACTCATAGTTCATACAGCAACGAACATAAAAATTTAGAAAGTTATCAATATTTAGAATTTTTAGGTGACTCAGTTTTAGGTTTTGTGGTTTCTGATTTCATTTTTGATAATTATAAAAATTTCGGTGAGGGAAAGGCAACAAAATTAAAAAGTGAAATTGTTGACAAAAATGCGCTTGGATTCTTATCGAGATATTATGGTTTTCCAAACTGTTTGAGGACAGGCAAAAGTGCATTTGCAAACGGAAAAGGAATTAAATCAGATTCAGATCTAATTGAATCATTCATTGGTGCTTGTTATAAAGCCTGCGGCTTGAAATTTACTAAAGAAATTATTTATCATTTTATTGACAAAATATTAAAAAAATTTCACAATTCAATCAATGAACACCCTAAAACAAAATTGCAAGAACTTGTACAAAGCAATTGA", "aa": "MKNNFEENLQNALLKFGINFQELNQQKRHYFYNAFTHSSYSNEHKNLESYQYLEFLGDSVLGFVVSDFIFDNYKNFGEGKATKLKSEIVDKNALGFLSRYYGFPNCLRTGKSAFANGKGIKSDSDLIESFIGACYKACGLKFTKEIIYHFIDKILKKFHNSINEHPKTKLQELVQSN", "aa_hexdigest": "483ef737bf205a06d730e69b5b04d867", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rnc" ], "truncated": "3-prime", "pseudogene": { "start": 592097, "stop": 592792, "observations": { "mutation": [ 592628 ], "stop": [ 592628 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8Q7", "query_cov": 1.0, "subject_cov": 0.7662337662337663, "identity": 0.966, "score": 343.0, "evalue": 1.06e-118, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MKNNFEENLQNALLKFGINFQKLDEQKRRYFCNAFTHSSYSNEHKNLESYQYLEFLGDSVLGFVVSDFIFDNYKNFGEGKATKLKSEIVDKNALGFLSRYYGFPNCLRTGKSAFANGKGIKSDSDLIESFIGACYKACGLEFTKEIIYHFIDKILKKFHNSINEHPKTKLQELVQSNWGCRPRYEDQALNNGKFESKVFINNNLYGEGIGQSKKESQTKAANDALKKMAKI" }, "paralog": false, "identity": 0.961038961038961, "score": 454.0, "evalue": 1.36e-164, "description": "Internal stop codon at 592628. Nonsense mutation around 592628.", "psc": { "uniref90_id": "UniRef90_D1J8Q7", "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_D1J8Q7", "cog_id": "COG0571", "cog_category": "K", "go_ids": [ "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8Q7", "EC:3.1.26.3", "UniRef:UniRef50_D1J8Q7", "COG:COG0571", "COG:K", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8Q7" ], "product": "Ribonuclease 3" } }, "id": "AHCKFKLKGB_1018", "locus": "GCA013367975_01017" }, { "type": "cds", "sequence": "contig_1", "start": 592814, "stop": 595750, "strand": "+", "frame": 2, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0005524", "GO:0007059", "GO:0030261", "RefSeq:WP_044420802.1", "SO:0001217", "UniParc:UPI0005CA2081", "UniRef:UniRef100_UPI0005CA2081", "UniRef:UniRef50_A0A448ZX91", "UniRef:UniRef90_D1J8Q8" ], "nt": "ATGAAGTTAATTCAAGTTGAAGCACACGGTTTTAAATCATTTGCAGATAAAGTAACCCTAAAATTTGACGGTGGAATTGTTGCAATAATAGGGCCTAATGGTTCAGGAAAATCAAACATAAATGACGCAATCAGATGGGTGTTGGGTGAAACTAGTTCCAAAGTATTGCGTGGAGATACAATGGAAGACGTTATTTTTTCAGGTTCTAAGACTGAAAAAGAAATGGATCGAGCAGAAGTAATTTTAACATTTGACAATAAGGATAGATCTTGTGATATCCCATATGATGTTTTTACTATTTCTAGAGTATTAACAAGGGGAAATGGTTCAAACGAATATTACATTAATGGTGAATTAGCAAGGCAAAGAGACATTAAAGAAATTGCAATGCAAAGTGGTATTTCAAAATCAAGTTTAGCAATCATAGGTCAAGGAACTATTTCCAACATTGCCGAATCAACTCCTGAAAAACGTAGAGAAATATTTGAAGATGCTGCTGGAACTTCAATGTACAAAAGCAAGAAGATTGAAGCACAAAGAAAATTAGAAAAAACCCAAGAAGCATTGGAACAAATTTCAATATTAATTCAAGAACAAGAAAGACAATTAAAGCCATTGCAACGTCAAGCTGAAAAAGCAAAAATTTATAAAACAAAAGCAGAGCAATTAAAAGAAGTCGAAGTTGCATTATTGGTTCATGATTTCATGGATTATTCAGATAAATTAGAAAAATTAAATGTTGAGGGGCAAGATTACAATCTAATTAAAGAAGAATTAGAAACCAAAATAAGAGTATACAACGAAGCAAGCGAACAAAAAAGCAAGTTTGTTTTAGAATTAGAAAACAACATTAAAAAGATAAGCGAAAGATTAAGCGAAATTAGCGAAGAAATTACGACCCTAGAAATAAGAAACGCAAAAGAAGCAAAACACCGCGAAATGCTTTTAAGTGGCGAACTAACTATATCCCCAAAAGAAAAATTAGCAGCAATGAAAGAAGAATTGAATGCATTAAATACTAATATTTTAGGGTATAAGACATTCTTAGAAACTAAAAAGAAGGAATCTGAACAATTATCTCAATCAGCTTCAATTAGATCAACAAAAATAAATGATATAAAACGAGAATTATCCTTTAAAAAAGAAGAACTTAATAAGATAAAATCAAAATTAGATGTTATTGAAGAAATAAAATCTAAAAAAACAAATTTGGCTAAAGGAACACATAATATTGTTGAAAACTCTCATTTATTTAGAGGATATAAAGCATTGGTTAGTGAATTAATTGAAGTTAATGAAAAATACGCCTTAGCTATTGAAACAATTCTAAGCTCCGCTATGCAACATATAGTTGTTGACAAACCAGAAACAGCAGTTGAAGCAATCAATTTCTTAAAATCAAATAATGGTGGTAGAGCAACTTTTATTCCCTTATCATCAATTAAACCTAAATTCGTTCCTGAACAACATATTGTTGTTGCTCAAACCCAAAAAGGTTTTCTAGGAATTGCTTCGGAACTTGTTTCAACAGAGGAACAATATAATGTTTTAAAGAAATTCTTACTAGGCAATGTCTTAGTTGCAGATAACATTGAAAATGCAAATAAATTATCCAAATTACTTGAAACTCGTTATATGGTTGTAACGCTAGATGGTGACATCATAAGAGTTGGTGGAGTTCTTTCAGGTGGACAAGCAAGTCAAAACGTTTCCACATTAGCAGCTGATGAACAAGTTAAAAAATTACAAGACCTTATTCCAGTAATTAATGCAAAAATTTCACAATTATCAAACGAGTTGTTTGAATTAGAAAAAGATCAAAATAAAGAAACATCATTATTGACAAATATTTCTCTTGAAAGAGCAAAGATTGACAACTTATATAGAACAACATTGGATAAGTTTGATTCATTACGTGAACAATATCGTAGTGCTTCGCAAGAAGAATATGAAGCAGAAAACAATAAAAAAATTGATATAGTAGCAGCAATTCAACAATTAATGCTTGAAAGAAGTGATTTATCGGCTAAAAAATCATCTCAAGAAGATATTTATCATAATCTAAAACAAGAATTACAAAATCTTACCAATCAACGCTTTCAAGTTAGTGAAGAATTAAATAATTTGATTAAAGATAACGCTAACAAAATAGCAGAAAAAGCAAAGGCAGAAACAATTATTCAAAATGCAAAGCAAAGATTGTCAGAACAATATGGAATGCTATTTGAAGTGGCAAAACAATTTTATAATCCAGATATAGATTTTGAAGTAGCAAGAAAAACAGTTGCTGATTTGAAATTGGATATAAGAGAGTTAGGACATGTAAACCTTGATTCAATTAATCAATTAGAAGAACTTGAAACAAGATATAAAGAAGTAAAATCTCAAGAACAAGAAATAATTTCAGCAAAGACCACCATTGAAGATGCAATATCAGAAATGGATAAAATTATTATTGAAAGAATGACGCAAACAGTTACATTAGTTAATGCTGAATTCAAGCATGTGTTTGCAAAAATGTTTGGTGGAGGTATGGCTGAAATAAGATATACTGACCCTGAAAACATTCTTGAAACAGGAATTGACGTCATTGCTCAACCTCCAGGAAAATCTATTAAGAATTTAAAATTATTTAGTGGGGGTGAAAAGGCACTTATAGCTATTTCATTACTATTTTCAATATTAAAAGCTAAGCCATTGCCATTGTGTATCTTAGACGAAGTTGAAGCAGCACTAGATGAAGCAAACGTAATAAGATTTGCTGAATATTTACACACTCTAAAAAAAGATACTCAATTTATAGTGATTACTCACCGTCAAGGAACAATGGAAAGAGTAAACAAACTTTATGGCGCAACAATGCAAAAAAGAGGGGTTACAACATTTTTTGCAGTAAATCTTTCAGATGCAAAGAAAACTATTGAAAACAACAATTAA", "aa": "MKLIQVEAHGFKSFADKVTLKFDGGIVAIIGPNGSGKSNINDAIRWVLGETSSKVLRGDTMEDVIFSGSKTEKEMDRAEVILTFDNKDRSCDIPYDVFTISRVLTRGNGSNEYYINGELARQRDIKEIAMQSGISKSSLAIIGQGTISNIAESTPEKRREIFEDAAGTSMYKSKKIEAQRKLEKTQEALEQISILIQEQERQLKPLQRQAEKAKIYKTKAEQLKEVEVALLVHDFMDYSDKLEKLNVEGQDYNLIKEELETKIRVYNEASEQKSKFVLELENNIKKISERLSEISEEITTLEIRNAKEAKHREMLLSGELTISPKEKLAAMKEELNALNTNILGYKTFLETKKKESEQLSQSASIRSTKINDIKRELSFKKEELNKIKSKLDVIEEIKSKKTNLAKGTHNIVENSHLFRGYKALVSELIEVNEKYALAIETILSSAMQHIVVDKPETAVEAINFLKSNNGGRATFIPLSSIKPKFVPEQHIVVAQTQKGFLGIASELVSTEEQYNVLKKFLLGNVLVADNIENANKLSKLLETRYMVVTLDGDIIRVGGVLSGGQASQNVSTLAADEQVKKLQDLIPVINAKISQLSNELFELEKDQNKETSLLTNISLERAKIDNLYRTTLDKFDSLREQYRSASQEEYEAENNKKIDIVAAIQQLMLERSDLSAKKSSQEDIYHNLKQELQNLTNQRFQVSEELNNLIKDNANKIAEKAKAETIIQNAKQRLSEQYGMLFEVAKQFYNPDIDFEVARKTVADLKLDIRELGHVNLDSINQLEELETRYKEVKSQEQEIISAKTTIEDAISEMDKIIIERMTQTVTLVNAEFKHVFAKMFGGGMAEIRYTDPENILETGIDVIAQPPGKSIKNLKLFSGGEKALIAISLLFSILKAKPLPLCILDEVEAALDEANVIRFAEYLHTLKKDTQFIVITHRQGTMERVNKLYGATMQKRGVTTFFAVNLSDAKKTIENNN", "aa_hexdigest": "dd5b17cc858c6b6c08383d3b2332d145", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0005CA2081", "ncbi_nrp_id": "WP_044420802.1", "uniref100_id": "UniRef100_UPI0005CA2081", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005CA2081", "RefSeq:WP_044420802.1", "UniRef:UniRef100_UPI0005CA2081" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005CA2081", "uniref90_id": "UniRef90_D1J8Q8" }, "psc": { "uniref90_id": "UniRef90_D1J8Q8", "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_A0A448ZX91", "cog_id": "COG1196", "cog_category": "D", "go_ids": [ "GO:0005524", "GO:0007059", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZX91", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZX91" ], "product": "Chromosome partition protein Smc" }, "genes": [ "smc" ], "id": "AHCKFKLKGB_1019", "locus": "GCA013367975_01018" }, { "type": "cds", "sequence": "contig_1", "start": 595986, "stop": 596381, "strand": "+", "frame": 3, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001319C362", "UniRef:UniRef90_UPI001319C362" ], "nt": "ATGAAAAAGTCTAAGAAAATATTATTAACTATGGGGGTAGTGCTTCCTTTATTAGCCTCAACTCCATTAGTTGCTGCTGGATGTGTAGAAACAAAGAAACCCGAAGTTAAACCAGAAGGTGAAAAACCAGGAACAACTCCTGAAAAGAAGCCTGAAGGTGAAAAACCAGGAACAACTCCTGAAGTTAAGCCAGAAGGTGAAACCACAATAGAAAACATCATTAAAAATCATAATGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTACTAAATGAATTAAATAAAGAAATTGAAAAAAGAGAAATTGAAAAAAGAGAAATTGAAAAAAGAGAAGAACAAAACCTTTTAGAAAAAAGAAACAACTTAATAAAAGAAACAGAAGCTAAATGA", "aa": "MKKSKKILLTMGVVLPLLASTPLVAAGCVETKKPEVKPEGEKPGTTPEKKPEGEKPGTTPEVKPEGETTIENIIKNHNEIKDDELNKTKSLLNELNKEIEKREIEKREIEKREEQNLLEKRNNLIKETEAK", "aa_hexdigest": "3781075ffac100c1fc32690e240a131c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 595986, "stop": 596682, "observations": { "insertion": [ 596664 ], "mutation": [ 596379 ], "stop": [ 596379 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001319C362", "query_cov": 1.0, "subject_cov": 0.5874439461883408, "identity": 0.8240000000000001, "score": 144.0, "evalue": 4.77e-41, "gene_start": 1, "gene_end": 131, "reference_start": 1, "reference_end": 121, "reference_sequence": "MKKSKKILLTIGIAFPLLASTPLVAAGCVKTNNPEAKPDDKKPGTTPEKKPEGEKPGTTPEVKPEGETTIENIIKNHNEIKDDELNKTKSLLNELNKEIEKREEQNLLEKRNNLIKEIEAKWDDITKKIEEYSKDATISKFPEFTQITDYLKYKNVELKNLKATKNNTIKLLVAKYFNILEAKILVENIIKNHNEIKDDELNKTKSLLNELNKEIEKEKNKTF" }, "paralog": false, "identity": 0.8917748917748918, "score": 342.0, "evalue": 3.29e-121, "description": "Internal stop codon at 596379. Frameshift due to insertion around 596664. Nonsense mutation around 596379.", "psc": { "uniref90_id": "UniRef90_UPI001319C362", "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI001319C362", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001319C362", "UniRef:UniRef50_UPI001319C362" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001319C362", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001319C362" ], "product": "variable surface lipoprotein" } }, "id": "AHCKFKLKGB_1020", "locus": "GCA013367975_01019" }, { "type": "cds", "sequence": "contig_1", "start": 596529, "stop": 596729, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGCCAAATATTTTAACATTTTAGAAGCCAAGATTCTTGTTGAAAACATCATTAAAAATCATAATGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTACTAAATGAATTAAATAAAGAAATTGAAAAAAGAGAAGAACAAAACCTTTTAGAAAAAAGAAACAACTTAATAAAAGAAACAGAAGCTAAATGA", "aa": "MVAKYFNILEAKILVENIIKNHNEIKDDELNKTKSLLNELNKEIEKREEQNLLEKRNNLIKETEAK", "aa_hexdigest": "02c4d3ca9c7465d430db3c6a5d4ad842", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7878.964699999996, "isoelectric_point": 5.851653861999511 }, "id": "AHCKFKLKGB_1021", "locus": "GCA013367975_01020" }, { "type": "cds", "sequence": "contig_1", "start": 596877, "stop": 597077, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGCCAAATATTTTAACATTTTAGAAGCCAAGATTCTTGTTGAAAACATCATTAAAAATCATAATGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTACTAAATGAATTAAATAAAGAAATTGAAAAAAGAGAAGAACAAAACCTTTTAGAAAAAAGAAACAACTTAATAAAAGAAACAGAAGCTAAATGA", "aa": "MVAKYFNILEAKILVENIIKNHNEIKDDELNKTKSLLNELNKEIEKREEQNLLEKRNNLIKETEAK", "aa_hexdigest": "02c4d3ca9c7465d430db3c6a5d4ad842", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7878.964699999996, "isoelectric_point": 5.851653861999511 }, "id": "AHCKFKLKGB_1022", "locus": "GCA013367975_01021" }, { "type": "cds", "sequence": "contig_1", "start": 597225, "stop": 597455, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGCCAAATATTTTAACATTTTAGAAGCCAAGATTCTTGTTGAAAACATCATTAAAAATCATAATGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTACTAAATGAATTAAATAAAGAAATTGAAAAAAGAGAAATTGAAAAAAGAGAAGTTGAAAAAAGAGAAGAACAAAACCTTTTAGAAAAAAGAAACAACTTAATAAAAGAAACAGAAGCTAAATGA", "aa": "MVAKYFNILEAKILVENIIKNHNEIKDDELNKTKSLLNELNKEIEKREIEKREVEKREEQNLLEKRNNLIKETEAK", "aa_hexdigest": "2a7dcb74713e597ca58a49648d57ebde", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9176.425299999992, "isoelectric_point": 5.92599925994873 }, "id": "AHCKFKLKGB_1023", "locus": "GCA013367975_01022" }, { "type": "cds", "sequence": "contig_1", "start": 597603, "stop": 598121, "strand": "+", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZ40", "UniRef:UniRef90_UPI0017464D24" ], "nt": "TTGGTTGCCAAATATTTTAACATTTTAGAAGCCAAGATTCTTGTTGAAAACATCATTAAAAATCATAATGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTACTAAATGAATTAAATAAAGAAATTGAAAAAAATCAAGCATTAAGCGAAGCAAAGAAACAATTAGATTCAGCCCTAAAACACGCAAAAAAATTAAATATAGGGTCGCTTGATTTTAACAAAAAATTTAATTTTAATAAAGAATTTAATAATGAATATGCGAGGGCATTAGAAGCAATTAAAGATAACGATTTGAATGCTATTAAAGAAATTACATTCGAATTAAATAGTCTTATAAATTTAACGACATCACAAAAATCAAAAACAACATCAGAAATTAAAAACGATGTTGAAGGCGAAGTTCATGAATTAAATGATTTAATAGCAAAATTAAATGATTTTGTTTCTAATTATGTTGAAGAAACATTTAATGACATAGATGAAGCGGGTTTAATTAGTTCAATAGATAATTGA", "aa": "MVAKYFNILEAKILVENIIKNHNEIKDDELNKTKSLLNELNKEIEKNQALSEAKKQLDSALKHAKKLNIGSLDFNKKFNFNKEFNNEYARALEAIKDNDLNAIKEITFELNSLINLTTSQKSKTTSEIKNDVEGEVHELNDLIAKLNDFVSNYVEETFNDIDEAGLISSIDN", "aa_hexdigest": "9b3ca214878377a5214a14957f521f33", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 597618, "stop": 598424, "observations": { "mutation": [ 598119, 598239 ], "stop": [ 598119, 598239 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0017464D24", "query_cov": 0.9709302325581395, "subject_cov": 0.5818815331010453, "identity": 0.9640000000000001, "score": 286.0, "evalue": 2.78e-95, "gene_start": 6, "gene_end": 172, "reference_start": 1, "reference_end": 161, "reference_sequence": "FNILEAKILVENIIKNHNEIKDDELNKTKSLLNELNKEIEKNQALSEAKKQLDSALKHAKKLNIGSLDFNKKFNNEYARALEAIKDNDLNAIKEITFELNSLINLTTSQKSKTTSEIKNDVEGEVHELNDLIAKLNDFVSNYVEETFNDIDEAGLISSIDNWIDMVEGFIKESKNPSKSLYFKLEYLKSQTKTFYEQKMESWANKVSDLLIEKGYDLLDKIANMKMVKNLAGYQKELEDCLNEFAELDETITVEKQLELSKKLYDLIPKIQKELDNEHNTTLNNELN" }, "paralog": false, "identity": 0.9626865671641791, "score": 489.0, "evalue": 8.61e-178, "description": "Internal stop codon at 598119, 598239. Nonsense mutation around 598119, 598239.", "psc": { "uniref90_id": "UniRef90_UPI0017464D24", "uniref50_id": "UniRef50_A0A6A8PZ40", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0017464D24", "UniRef:UniRef50_A0A6A8PZ40" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZ40", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZ40" ], "product": "Variable surface lipoprotein" } }, "id": "AHCKFKLKGB_1024", "locus": "GCA013367975_01023" }, { "type": "cds", "sequence": "contig_1", "start": 598128, "stop": 598241, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGAAGGTTTTATTAAAGAATCTAAAAATCCTTCTAAATCGCTATATTTTAAATTAGAATATTTGAAATCTCAAACAAAAATATTTTATGAACAAAAAATGGAATCATGA", "aa": "MVEGFIKESKNPSKSLYFKLEYLKSQTKIFYEQKMES", "aa_hexdigest": "c9e4b444aaf879f8efb3e8c2b33e021c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4480.161300000001, "isoelectric_point": 9.050087928771973 }, "id": "AHCKFKLKGB_1025", "locus": "GCA013367975_01024" }, { "type": "cds", "sequence": "contig_1", "start": 598287, "stop": 598499, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAAAATTGCAAATATGAAAATGGTTAAAAACCTTGCCGGATATCAAAAAGAATTGGAAGATTGCTTAAATGAATTTGCTGAACTAGATGAAACCATTACCGTAGAAAAACAATTTGAGTTGAGCAAAAAACTTTATGATTTAATACCAAAAATTCAAAAAGAATTAGACAACGAACATAATAAGACCTTAAATAATGAATTAAATTAA", "aa": "MDKIANMKMVKNLAGYQKELEDCLNEFAELDETITVEKQFELSKKLYDLIPKIQKELDNEHNKTLNNELN", "aa_hexdigest": "448a5b005a27b13430da36596cb32525", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8258.345499999996, "isoelectric_point": 4.774384498596191 }, "id": "AHCKFKLKGB_1026", "locus": "GCA013367975_01025" }, { "type": "cds", "sequence": "contig_1", "start": 598729, "stop": 598848, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGGGTTAGTTTTTTATTTAGATAAAGAAATATTAAAAAAAGAATACTCAGATAGTAATCATAAAGAAGCTTATGAAAAAGTAAGAGATATATTGAGGCACTACGGATTTCATTGA", "aa": "MYGLVFYLDKEILKKEYSDSNHKEAYEKVRDILRHYGFH", "aa_hexdigest": "3e2077c335083dcd249c2a105b696001", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4836.4392, "isoelectric_point": 6.817859840393066 }, "id": "AHCKFKLKGB_1027", "locus": "GCA013367975_01026" }, { "type": "cds", "sequence": "contig_1", "start": 599221, "stop": 599898, "strand": "-", "frame": 1, "gene": null, "product": "Leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R0", "UniRef:UniRef90_D1J8R0" ], "nt": "TTGCTAGATTTATCAAAAACCAATATAACTTCAATACCCGCAGGATCATTTTCTTATCTAGTTATGCAAAATTTGGCAGACGATGGTGCTGGCTTACCAACTGAATTATCAACATTAGTTACAACTAATTCTTCAAATAACAACAAAAAAATAAATATTAGAAAAATCATTTTGCCAAAAACCTTAAAATCAATTGGCGCAGGTGCTTTTTATGGATTAAGTAACCTAGAAGAAGTAGATTTTTCTCAATGCAACGACTTATCTCAAATAGATGCTGAAGCTTTTTCAAATACAAATATTAAAAAATTACAATTGCCTGCATCTTTAAATAAGTTAGGACAAAAAGCCTTTGCAAACAGTGGCATTACATCTGTAAATTTAGAAGATTTACCTTATTTAAAAACTCTTACACAAGGTGTTTTTGCTTCAAATAAGCTTACAAGCATAAACTTAAAAAACATTGAATTAGTTGAAAGCGGAGCTTTGGCAGATAATCAAATAAAAACAATAGAAATTCCTTCAACAATGAAATCATTTAGTCCTGAATCATTTGACTTTTACGGCGCAGGGGAATCAGCTGCTAAAACTCTTATTTCTAAAATTGAAAACAATGATATTCAATCGTTTTTAGAAAAAGCTTTGAAGGAAAACCCAAATCATCTATATAAATTAAACTAA", "aa": "MLDLSKTNITSIPAGSFSYLVMQNLADDGAGLPTELSTLVTTNSSNNNKKINIRKIILPKTLKSIGAGAFYGLSNLEEVDFSQCNDLSQIDAEAFSNTNIKKLQLPASLNKLGQKAFANSGITSVNLEDLPYLKTLTQGVFASNKLTSINLKNIELVESGALADNQIKTIEIPSTMKSFSPESFDFYGAGESAAKTLISKIENNDIQSFLEKALKENPNHLYKLN", "aa_hexdigest": "612dca26dc65ca350270c8628a3dd550", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 599221, "stop": 600084, "observations": { "mutation": [ 599916 ], "stop": [ 599916 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8R0", "query_cov": 1.0, "subject_cov": 0.7839721254355401, "identity": 0.9420000000000001, "score": 379.0, "evalue": 5.1e-131, "gene_start": 1, "gene_end": 225, "reference_start": 63, "reference_end": 287, "reference_sequence": "MKKNKKFLFLGFGLVAPFIAGLTAISCGGEKFNAFALNHVQGPLKDPKNSTNHNQTWFAESGLLDLSKTNITSIPAGAFSYLVMQNLADEGAGLPTELSTLVTTNSSNNNKKINIRKIILPKTLKSIGAGAFYGLSNLEEVDFSQCNDLSQIDAEAFSNTNIKKLQLPASLNKLGQKAFANSGITSVNLEDLPYLKTLTQGVFASNKLTNINLKNIELVESGALADNQIKTIEIPSTMKSFSPESFDFIGAGESAAKTLISKIENHEIESFLKDALKKNPNHLYKLS" }, "paralog": false, "identity": 0.9512195121951219, "score": 504.0, "evalue": 5.27e-183, "description": "Internal stop codon at 599916. Nonsense mutation around 599916.", "psc": { "uniref90_id": "UniRef90_D1J8R0", "product": "Leucine-rich repeat domain-containing protein", "uniref50_id": "UniRef50_D1J8R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8R0", "UniRef:UniRef50_D1J8R0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R0" ], "product": "Leucine-rich repeat domain-containing protein" } }, "id": "AHCKFKLKGB_1028", "locus": "GCA013367975_01027" }, { "type": "cds", "sequence": "contig_1", "start": 599914, "stop": 600084, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGAACAAAAAATTTTTATTTTTAGGCTTTGGTTTAGTTGCTCCATTTATTGCTGGATTAACAGCAATATCTTGTGGAGGAGAAAAGTTCAATGCTTTTGCTCTAAATCATGTGCAAGGCCCACTAAAAGACCCTAAGAACTCATCCAATCACAATCAAACTTGA", "aa": "MKKNKKFLFLGFGLVAPFIAGLTAISCGGEKFNAFALNHVQGPLKDPKNSSNHNQT", "aa_hexdigest": "b8eb387af183d42d9da3b0f1b50a8ec6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6047.9625000000015, "isoelectric_point": 9.903455924987792 }, "id": "AHCKFKLKGB_1029", "locus": "GCA013367975_01028" }, { "type": "cds", "sequence": "contig_1", "start": 600178, "stop": 602148, "strand": "+", "frame": 1, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "EC:3.1.25.-", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887", "RefSeq:WP_175933589.1", "SO:0001217", "UniParc:UPI001588E7C1", "UniRef:UniRef100_UPI001588E7C1", "UniRef:UniRef50_B3PLX9", "UniRef:UniRef90_D1J8R1" ], "nt": "ATGGAAAAGTTTAAATTAGTTTCAAATTATAAGCCAGCCGGCGACCAACCTCAGGCAATAAATGAGTTGGTTCACGGTGTTTTAGAAGGCAAAGAGCATCAAGTTTTATTAGGCGTAACTGGTTCAGGAAAAACATTTACTATGGCAAATGTCATTAATCAGTTAAATAGGCCTACATTGGTTATAAGCCATAACAAAACATTAGCAAGTCAACTTTATAGCGAATTAAGGGAATTATTTCCTGAAAATAGAGTTGAATATTTTGTTTCGTATTTTGATTTTTATAAGCCAGAAGCATACATTCCTAAGTCTGATTTATATATCGAAAAAACAAGTAGAAACAATAAAGATTTAGAAGCAATGAGAATGTCTGCTCTTAACGCCTTAAGTATTCGAAGAGATACTATTGTTGTTGCATCTGTTGCTGCAATATATGCCGAATTTAATCCTAAAGAATATATTCAAAGTTTTATGCCTATTGAAAGCCATATGAAAATTAAGTTGCGTGATCTTTTAACCAAGTTGGTTAATTTAGGTTATGAAAAACATAATAGTGATTTATCTTCTGGACAATTTTTCAATAAGGGGGAAGTTATTGAAATTTGTCCCGGATATACTTCAGAAAACAATATTCGTATTGAGATGTTTGATGATTATATTGAAAGAATTGCATTAATTAATCCTTTAACAAAGTCAATAGTTGAAGTGCGTAAGTCGTTGACTATTTTTCCTGCAACTACCTATAACGTAGAAAAAGGTTATATTGAAAATATTTGCAATAGCATTGAAAGCGAATTAAAAGAAAGAGTTGCCTATTTTAAATCACAAAATCAACTTTTGGAAGCCCAAAGGATTCACGATAAAACTTTAAATGATATTGATTCATTAAAAGAATTCGGTTATGTTAAAGGCATTGAAAATTATGCTCGATATGTTGATAAACGTGAACCCGGGCAAAAACCATACACAATATTTGATTATTTGCCAAAGGATGCAGTAATTTTTGTTGATGAAAGTCACCTTATGATTCCTCAAATAATAGGAATGTACGAAGGAGATCGTTCTAGGAAAGAAACACTAATTAAATATGGTTTTAGATTACCTTCTGCATTAGATAATAGACCCTTAAAATTTAATGAATATGAAGAATATAAATTTCCAAGAATTTATATGTCAGCAACCCCCGGTGACTATGAAATAAATAAGGCCAATGGTGAACTAGTTACTCAATACATTAGACCAACAGGCCTTTTAGATCCCATTATTGAAATACATTCAAAAGAAAATCAAATTCAAGACATTCATGATTTATTAAAATTACAAATAGAAAAAAAAGAAAGAACTTTAATTTTAACTACAACTAAAAAGAATGCCGAAGAACTTTCTTTATACCTTTCTGAATTTAAGATTAAAAGCGCATATATTCACGATGGATTTAAAACCTTTGAGAGAAACGAAATCTTAAAAGGTTTAAGAATGGGAAAATATGATGTTGTAATTGGAATTAATTTATTGCGTGAAGGTATTGATTTGCCAGAAGTTAGTTTAATTTGCGTATTGAATGCAGATTCTATGGGGCTAATGCGCGATGCTAGAAGTTTGATTCAAATAGTTGGCAGGGCATCTAGAAACGACCATGGAAAAGTAATATTTTATGCTGATAATATTACAACTAGTATGCAAAAAGCAATTGTTGACAATGAACATAAAAGGACGTTGCAAATTAAATATAATAAAGAACACAACATTACCCCTAAAAGTATTATTAAACCAATACCCGCACCTATTCAAGGCAGTATTTTATTAGAAGATAATAAAGCAATAAAAGAAGCCAATAGGACTATTCATAATAAAAAATATATTGATAATATGATTAAACATATGAAAGAACTAGCGAGGGCAAAAAAATACGAAGAAGCTATTGAAATAAGAGATTATTTGATTGAATTAGGTGTCGAATTTGATGATTAA", "aa": "MEKFKLVSNYKPAGDQPQAINELVHGVLEGKEHQVLLGVTGSGKTFTMANVINQLNRPTLVISHNKTLASQLYSELRELFPENRVEYFVSYFDFYKPEAYIPKSDLYIEKTSRNNKDLEAMRMSALNALSIRRDTIVVASVAAIYAEFNPKEYIQSFMPIESHMKIKLRDLLTKLVNLGYEKHNSDLSSGQFFNKGEVIEICPGYTSENNIRIEMFDDYIERIALINPLTKSIVEVRKSLTIFPATTYNVEKGYIENICNSIESELKERVAYFKSQNQLLEAQRIHDKTLNDIDSLKEFGYVKGIENYARYVDKREPGQKPYTIFDYLPKDAVIFVDESHLMIPQIIGMYEGDRSRKETLIKYGFRLPSALDNRPLKFNEYEEYKFPRIYMSATPGDYEINKANGELVTQYIRPTGLLDPIIEIHSKENQIQDIHDLLKLQIEKKERTLILTTTKKNAEELSLYLSEFKIKSAYIHDGFKTFERNEILKGLRMGKYDVVIGINLLREGIDLPEVSLICVLNADSMGLMRDARSLIQIVGRASRNDHGKVIFYADNITTSMQKAIVDNEHKRTLQIKYNKEHNITPKSIIKPIPAPIQGSILLEDNKAIKEANRTIHNKKYIDNMIKHMKELARAKKYEEAIEIRDYLIELGVEFDD", "aa_hexdigest": "2cb6c4e05675a06395e122a6b4b1b420", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001588E7C1", "ncbi_nrp_id": "WP_175933589.1", "uniref100_id": "UniRef100_UPI001588E7C1", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588E7C1", "RefSeq:WP_175933589.1", "UniRef:UniRef100_UPI001588E7C1" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588E7C1", "uniref90_id": "UniRef90_D1J8R1" }, "psc": { "uniref90_id": "UniRef90_D1J8R1", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "ec_ids": [ "3.1.25.-" ], "uniref50_id": "UniRef50_B3PLX9", "cog_id": "COG0556", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLX9" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "AHCKFKLKGB_1030", "locus": "GCA013367975_01029" }, { "type": "cds", "sequence": "contig_1", "start": 602347, "stop": 603012, "strand": "-", "frame": 1, "gene": null, "product": "Type-2 restriction enzyme", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2S0NKX5" ], "nt": "GTGAATTTCCAAAAAGTTTATGAAAATATTGAAAAAATAAAAATTGAATTATGCATTTTAAATTCATTAATTAAATCAAAAAATATAAAAGCAGATTTTAAAAATTTAATACAAATGTATCCTAATGTATTAAAAGCCATACCAACTTTATTAGCAATAAGAGGTAATAATATTCGAGTTGGAATTGAAAAAGAAATTCTAATTAATTTTGATAAAAAGCTAAATAGTATAGAAGAATATACACTATTTATGGAAAAGACAGGCTTGTTTGAATTAATAAGGAATAATATAAACAATAGCCTTGTTGACTATGCAACTGGTGTTGAAGTCGGAATTGATACAAATACAAGAAAAAACAGAACAGGCTCAATGATGGAAAACCTTGTTGAACATTATTTAATAAATTCAAATTTAATTGAAAATGTTAACTATTTTAAACAAATTGATTTAAATAGCTTTCAAAAGAAAACGAATATTAATATTGACCCCACATTATTAAATATAAATAAAAAATTTGACTTTATTTTTATAAATAATCAAAATCATATTTTTGTATGCGAATGTAATTTTTACAATACAAGTGGTTCAAAATTGAATGAGGTTGCCAGAAGTTATAGAATTTTGTCAAGGTTATTTGATGAGACTGGAAATATTACATTTGTTTGA", "aa": "MNFQKVYENIEKIKIELCILNSLIKSKNIKADFKNLIQMYPNVLKAIPTLLAIRGNNIRVGIEKEILINFDKKLNSIEEYTLFMEKTGLFELIRNNINNSLVDYATGVEVGIDTNTRKNRTGSMMENLVEHYLINSNLIENVNYFKQIDLNSFQKKTNINIDPTLLNINKKFDFIFINNQNHIFVCECNFYNTSGSKLNEVARSYRILSRLFDETGNITFV", "aa_hexdigest": "f9b19c786be33b354e366b7351bfe1cc", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0030CDD1ED", "query_cov": 0.9638009049773756, "subject_cov": 0.8452380952380952, "identity": 0.507, "score": 195.0, "evalue": 3.56e-59, "valid": false, "product": "type II restriction endonuclease", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_A0A2S0NKX5", "kegg_orthology_id": "K01155" }, "pscc": { "uniref50_id": "UniRef50_A0A2S0NKX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2S0NKX5" ], "product": "Type-2 restriction enzyme" }, "genes": [], "id": "AHCKFKLKGB_1031", "locus": "GCA013367975_01030" }, { "type": "cds", "sequence": "contig_1", "start": 603054, "stop": 603788, "strand": "-", "frame": 2, "gene": null, "product": "Site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "EC:2.1.1.72", "KEGG:K06223", "SO:0001217", "UniRef:UniRef50_N9U9M3", "UniRef:UniRef90_A0A6A8Q0M8" ], "nt": "TTGCCTAAGAATTTTTGCATAAATGATATTAACAGCGAACTAGTTCTAGCATACAAGTGTTTTGCAAAGAAAGAATCATTTGATAAATTAAAATCACTTTTAGACCTTCATACTAGTAAGCATTCCAACGAATACTATTATCAAATAAGGAATTTAGATAGACAACCCGAATATAAAAACTTGTCAGATGAAGAAAAGGCTGCCAGAATTATTTATTTGAATAAAGCATGTTTTAATGGTTTATATCGTGTAAACAAAAATGGTTTTTTTAATGTTCCATCAGGAAAGAAAGAAAAAGTCAATTGCTATAATAAAGAAAACTATAATTCTATTTTTGAATATTTTTCTAATGTAGATTGTAAAATAACAAATTTAAATTTCGAAGATGCAATAAAAGACGCAAAAGCTGGCGACCTAGTTTATTTTGATCCACCTTATGATACCTTAGAAAATAAACATTCATTTACTTCTTATTCAAATGAAACCTTTGGAAAAAACGAGCAAATTAAACTTGCTAATGTTTTTAAAAAGTTGTCAAAAAAGGGCGTTTATGTAATATTAAGTAATCACAAAACTGATTTTGTTTGCAATTTATACAGGGATTTTAATATTAATATAATTAAAGCAAGAAGAAACATTAATAGAAATGGTAAAAATCGAGGCGATGTCGAAGAGGTAATCATTACTAATTTTAATAAAGAGGTTCTATGCAAACAAGATTTGAAAAATTTTTAA", "aa": "MPKNFCINDINSELVLAYKCFAKKESFDKLKSLLDLHTSKHSNEYYYQIRNLDRQPEYKNLSDEEKAARIIYLNKACFNGLYRVNKNGFFNVPSGKKEKVNCYNKENYNSIFEYFSNVDCKITNLNFEDAIKDAKAGDLVYFDPPYDTLENKHSFTSYSNETFGKNEQIKLANVFKKLSKKGVYVILSNHKTDFVCNLYRDFNINIIKARRNINRNGKNRGDVEEVIITNFNKEVLCKQDLKNF", "aa_hexdigest": "86934c57070a986bbe40615631809150", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A6A8Q0M8", "query_cov": 1.0, "subject_cov": 0.8472222222222222, "identity": 0.996, "score": 493.0, "evalue": 6.53e-176, "valid": true, "product": "Site-specific DNA-methyltransferase (adenine-specific)", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_N9U9M3", "kegg_orthology_id": "K06223" }, "pscc": { "uniref50_id": "UniRef50_N9U9M3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U9M3" ], "product": "Site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "AHCKFKLKGB_1032", "locus": "GCA013367975_01031" }, { "type": "cds", "sequence": "contig_1", "start": 604095, "stop": 604277, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATTATCATTATTTGCAAACGTTGAATATGACGAAAATAAATCATTGTATGAACAAGTTGTGCAATTGATAGAAGGAATAAAAAATACTAACTCGCCCAAAGCAAAATTATTAATTAACGAAATTATTGATTTATACAATTCTTATTTTTTAACCTTTGGAAGTAACGGATATTGA", "aa": "MKELSLFANVEYDENKSLYEQVVQLIEGIKNTNSPKAKLLINEIIDLYNSYFLTFGSNGY", "aa_hexdigest": "628d7164a97f09f7c075bd632dee2cbd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6923.756599999999, "isoelectric_point": 4.601082420349121 }, "id": "AHCKFKLKGB_1033", "locus": "GCA013367975_01032" }, { "type": "cds", "sequence": "contig_1", "start": 604899, "stop": 605468, "strand": "-", "frame": 2, "gene": null, "product": "MYPU_1760 family metalloprotease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D", "UniRef:UniRef90_UPI001E491E9D" ], "nt": "TTGAGCTATAAATTAGCAACTCCTGAACTAAACAAAATAATCAATAAATTCAATGGCAACAACAACAAATTTTTAACCGAATTTAGAAAAACGCTAAACTACGATGGTTCAATGCAAAACGATGAATATTTAGTCAATAAAGAATTATTTAATCCTAAAAACAAAGAAATACCAGTTTATTCAAAATTTAGTCAATATGACTTATTCAAGTTAGCTAACCTCACCTTAACAAACAAAGAAGTTGAAAAATACAATATTTTAAACAATAAAAACTTTTATTTTAACAATTCAACAAACAATACAGTTTATTATGCAAATTCTACTAATATTGATCAAATAAAATACTTGTTTTCGTTTGAGGAACTTTTTGCAAGAGAACTAGTAAAACTTTCATATTCAGCACATGAAAATTTTCAAATGTTTGACGGTCATTTAACTTTGGATGATTCTTTAAATTTCTTATATTATAAATTAAAAAATGATGATATTTTTCTATCATCAATTGGCGATGACACATTGAAAATGATTGCCAATTATTATAATAAATATGTAATATATTCAGCCAATTGA", "aa": "MSYKLATPELNKIINKFNGNNNKFLTEFRKTLNYDGSMQNDEYLVNKELFNPKNKEIPVYSKFSQYDLFKLANLTLTNKEVEKYNILNNKNFYFNNSTNNTVYYANSTNIDQIKYLFSFEELFARELVKLSYSAHENFQMFDGHLTLDDSLNFLYYKLKNDDIFLSSIGDDTLKMIANYYNKYVIYSAN", "aa_hexdigest": "db64911574005fdf261a57949ce8341e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 604880, "stop": 605768, "observations": { "insertion": [ 604907 ], "mutation": [ 605588 ], "stop": [ 605588 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E491E9D", "query_cov": 0.9894179894179894, "subject_cov": 0.4605911330049261, "identity": 0.973, "score": 343.0, "evalue": 1.28e-115, "gene_start": 1, "gene_end": 187, "reference_start": 212, "reference_end": 398, "reference_sequence": "MGNIYYNLGFYINGGIIGVVIYYIFNYVNYSNGNGGGLFDGQKNKNYSMPNEERYLQSFNAKTIVKNNIQLKNKNYQYGNLSIFEYPFALTKNNEPIYFLGEEGRKLLNLEFLKRGFYGPEINGLNHVYINKKIPNVSNIKDVNGVYLPQAFDIFIQINHFISGGIKQVQSWSLQKRVELVFNTLFHEYAHHIDNTYNRSIRENDEFANNDLSYKLATPELNKIINKFNGNNNKFLTEFRKTLNYDGSMQNDEYLVNKELFNPKNKEIPVYSKFSQYDLFKLANLALTNKEVEKYNILNNKNFYFNNSTNNTVYYANSTNIDQLKYLFSFEELFARELVKLSYSAHENFQMFDGHLTLDDSLNFLYYKLKNDDIFLSSIGDDTLKMIANYYNKYVIYSPIEYLMMN" }, "paralog": false, "identity": 0.9796610169491525, "score": 562.0, "evalue": 1.54e-204, "description": "Internal stop codon at 605588. Frameshift due to insertion around 604907. Nonsense mutation around 605588.", "psc": { "uniref90_id": "UniRef90_UPI001E491E9D", "product": "MYPU_1760 family metalloprotease", "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E491E9D", "UniRef:UniRef50_UPI001E491E9D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D" ], "product": "MYPU_1760 family metalloprotease" } }, "id": "AHCKFKLKGB_1034", "locus": "GCA013367975_01033" }, { "type": "cds", "sequence": "contig_1", "start": 605586, "stop": 606143, "strand": "-", "frame": 2, "gene": null, "product": "MYPU_1760 family metalloprotease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D", "UniRef:UniRef90_UPI001E491E9D" ], "nt": "ATGAGCAAGCAAAAACAAGGTAGAAAAGCATTTCTACCGAAATGGGCAATATTTATTATAATCTTGGCTTTTATATTAACAGCAGGTATTATTGGCGTCGTAATATATTACATTTTTAATTATGTTAATTATTCAAATGGCAATGGCGGGGGTTTATTTGATGGCCAAAAAAATAAAAATTATTCAATGCCAAATGAAGAAAGATATCTACAAAGTTTCAATGCAAAAACGATTGTCAAAAACAATGTTCAATTAAAGAACAAAAATTATCAATATGGAAATTTAAGTATTTTTGAATACCCTTTTGCTTTAACAAAAAATAATGAACCTATTTATTTTTTGGGTGAAGAAGGAAGAAAATTATTAAATTTAGAGTTTTTAAAAAGAGGCTTTTATGGTCCTGAAATTAATGGTTTAAATCATGTTTATATAAATAAAAAAATACCTAATGTTTCGAATATTAAAGATGTAAATGGTGTTTATTTGCCACAAGCTTTTGACATTTTTATTCAAATAAATCATTTTATCTCTGGAGGAATAAAACAAGTCCAATCATGA", "aa": "MSKQKQGRKAFLPKWAIFIIILAFILTAGIIGVVIYYIFNYVNYSNGNGGGLFDGQKNKNYSMPNEERYLQSFNAKTIVKNNVQLKNKNYQYGNLSIFEYPFALTKNNEPIYFLGEEGRKLLNLEFLKRGFYGPEINGLNHVYINKKIPNVSNIKDVNGVYLPQAFDIFIQINHFISGGIKQVQS", "aa_hexdigest": "4c02f145434d6cd7183bbe51a583d642", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 605283, "stop": 606102, "observations": { "insertion": [ 606063 ], "stop": [ 605588 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E491E9D", "query_cov": 0.8864864864864865, "subject_cov": 0.4039408866995074, "identity": 0.963, "score": 320.0, "evalue": 9.61e-107, "gene_start": 22, "gene_end": 185, "reference_start": 8, "reference_end": 171, "reference_sequence": "MGNIYYNLGFYINGGIIGVVIYYIFNYVNYSNGNGGGLFDGQKNKNYSMPNEERYLQSFNAKTIVKNNIQLKNKNYQYGNLSIFEYPFALTKNNEPIYFLGEEGRKLLNLEFLKRGFYGPEINGLNHVYINKKIPNVSNIKDVNGVYLPQAFDIFIQINHFISGGIKQVQSWSLQKRVELVFNTLFHEYAHHIDNTYNRSIRENDEFANNDLSYKLATPELNKIINKFNGNNNKFLTEFRKTLNYDGSMQNDEYLVNKELFNPKNKEIPVYSKFSQYDLFKLANLALTNKEVEKYNILNNKNFYFNNSTNNTVYYANSTNIDQLKYLFSFEELFARELVKLSYSAHENFQMFDGHLTLDDSLNFLYYKLKNDDIFLSSIGDDTLKMIANYYNKYVIYSPIEYLMMN" }, "paralog": false, "identity": 0.9852941176470589, "score": 549.0, "evalue": 2.77e-199, "description": "Internal stop codon at 605588. Frameshift due to insertion around 606063.", "psc": { "uniref90_id": "UniRef90_UPI001E491E9D", "product": "MYPU_1760 family metalloprotease", "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E491E9D", "UniRef:UniRef50_UPI001E491E9D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D" ], "product": "MYPU_1760 family metalloprotease" } }, "id": "AHCKFKLKGB_1035", "locus": "GCA013367975_01034" }, { "type": "cds", "sequence": "contig_1", "start": 606213, "stop": 606665, "strand": "+", "frame": 3, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "db_xrefs": [ "COG:COG0742", "COG:J", "EC:2.1.1.171", "GO:0031167", "GO:0052913", "KEGG:K08316", "SO:0001217", "UniRef:UniRef50_A0A077L643", "UniRef:UniRef90_A0A454CAI7" ], "nt": "ATGTTGAGAATAATAGCAGGAAAATATAGATCCAGAATTCTAAAGCAACCTTCCAAATCAATAACAAGACCAACAATAGATAGAGCGAGGGAGGCAATATTTTCTTCAATTCAGTTTAACATTGAAAATAAGAAAGTTTTAGATTTATTTTCGGGATCGGGAGCATTTTGTTTAGAAGCTCTATCAAGGGGCGCAAAATTCGCGGTATGTGTTGATAAAAATGCCGAGGCTATTAAAATAATTAAAGAAAATCAAAATAGTTTAAAAGAACCAAATTTAAAAATTTTTAATATTGATGCATTGAATTTTTTAAATAATAATGTTGAAGTATTTGACTATATTTATTTAGATCCCCCATTTGTTGAAAAAAAATTACTAGTTGAATGTTTAAATAAAATTATTGATAAAAAATGTATTGATAAAAGTGGGACAATGATAATTGAAACAGATTGA", "aa": "MLRIIAGKYRSRILKQPSKSITRPTIDRAREAIFSSIQFNIENKKVLDLFSGSGAFCLEALSRGAKFAVCVDKNAEAIKIIKENQNSLKEPNLKIFNIDALNFLNNNVEVFDYIYLDPPFVEKKLLVECLNKIIDKKCIDKSGTMIIETD", "aa_hexdigest": "b71f3fdbd296b995342c242285c41994", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAI7", "query_cov": 1.0, "subject_cov": 0.8152173913043478, "identity": 0.993, "score": 292.0, "evalue": 1.81e-99, "valid": true, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "ec_ids": [ "2.1.1.171" ], "uniref50_id": "UniRef50_A0A077L643", "cog_id": "COG0742", "cog_category": "J", "kegg_orthology_id": "K08316", "go_ids": [ "GO:0031167", "GO:0052913" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L643", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L643" ], "product": "N6-adenine-specific methylase" }, "genes": [ "rsmD" ], "id": "AHCKFKLKGB_1036", "locus": "GCA013367975_01035" }, { "type": "cds", "sequence": "contig_1", "start": 606819, "stop": 607442, "strand": "+", "frame": 3, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "COG:COG0194", "COG:F", "EC:2.7.4.8", "GO:0004385", "GO:0006163", "RefSeq:WP_012855758.1", "SO:0001217", "UniParc:UPI0001BC2503", "UniRef:UniRef100_D1J8S4", "UniRef:UniRef50_Q98PN5", "UniRef:UniRef90_D1J8S4" ], "nt": "ATGAGTAAAAAATTAATAATATTTACAGGACCAAGTGGCGTTGGAAAAGGAACAATTGAAAAGCCCTTATTTGAAGATAAAAGTCTAAAGCTAAAATTATCAGTATCAATGACAACGAGATCACCACGTGAAGGAGAAATTGATGGTGTACACTATTACTTTGTGTCAAAAGATACTTTTAACTCTTGCTTAGCAGAAAATAAGCTAATAGAATATTCAATGCACTTTGATAATTATTATGGAACATTATATTCAGAAATTGAACGTATTAGTGAACAAGGAAAAATTCCATTTCTTGAAATTGAAACTAATGGAGCTAAACAAATTTTAGAATATTATAAAAAACGTAATAGATTAGATGAAATTTGTTCAATATTTTTAATGCCTCCTTCATATGGAGAATTAAGAAAAAGAATTGAAGGTCGCAATACCGAAACTGAAGATCTAATTATTAAAAGAATGAAGAAAGCAAAAGAAGAAATAGGCTATTCTACAATATTTCATCATGTGATAGTTAATGATGATTTAAACGTAGCACTTGAAGAAATCAAAGAAATAATCAGATCAGAATTTTCAGATATTATAAACAAAAAAGAAGTTGCAAGTGAAGGTAGAAAGGCATAA", "aa": "MSKKLIIFTGPSGVGKGTIEKPLFEDKSLKLKLSVSMTTRSPREGEIDGVHYYFVSKDTFNSCLAENKLIEYSMHFDNYYGTLYSEIERISEQGKIPFLEIETNGAKQILEYYKKRNRLDEICSIFLMPPSYGELRKRIEGRNTETEDLIIKRMKKAKEEIGYSTIFHHVIVNDDLNVALEEIKEIIRSEFSDIINKKEVASEGRKA", "aa_hexdigest": "458f5bbf2abd014048df359e386016bd", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2503", "ncbi_nrp_id": "WP_012855758.1", "uniref100_id": "UniRef100_D1J8S4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2503", "RefSeq:WP_012855758.1", "UniRef:UniRef100_D1J8S4" ] }, "ips": { "uniref100_id": "UniRef100_D1J8S4", "uniref90_id": "UniRef90_D1J8S4" }, "psc": { "uniref90_id": "UniRef90_D1J8S4", "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_Q98PN5", "cog_id": "COG0194", "cog_category": "F", "go_ids": [ "GO:0004385", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN5" ], "product": "Guanylate kinase" }, "genes": [ "gmk" ], "id": "AHCKFKLKGB_1037", "locus": "GCA013367975_01036" }, { "type": "cds", "sequence": "contig_1", "start": 607536, "stop": 607679, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGCTTTGTGTGATGGTATGGGTGGACATGTTGGCGGTAGCCAATGTTCAACATTATCAACTAATAGTATTAAGCAATATTTCCTAGATACATTTCCTCAAAAGTTGGAATGTAGTGATAAAAAAAACGTTAGCAAATGA", "aa": "MAALCDGMGGHVGGSQCSTLSTNSIKQYFLDTFPQKLECSDKKNVSK", "aa_hexdigest": "897f954bd8244134008f6051081b9bdd", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5026.679600000001, "isoelectric_point": 7.717677879333496 }, "id": "AHCKFKLKGB_1038", "locus": "GCA013367975_01037" }, { "type": "cds", "sequence": "contig_1", "start": 607764, "stop": 608201, "strand": "+", "frame": 3, "gene": "pTC1", "product": "Protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_D1J8S5", "UniRef:UniRef90_D1J8S5" ], "nt": "ATGGTTGTTGCCCTCATTTTTAATGCAAATGGTTTAGCGTATGTCTTTAATATTGGCGATTCACGCTTGTATGCATACAATGGATTACTTTATCAAATCACAGAAGACCAGAATTATTTATATCAATTGATGAGAGAATTTAATTTAACATACGAAGAAGCAGCATTAGATCCTAATTCTTACAAACTTATAAGCTGCCTAGGCCCTAATAAAAAAACCAATTGTCAATCATTTTTTATATCACAAAAATCAGCAGTTAAATATTATTTATTAACATCCGATGGATTACACGATTATGTTTCTAAACCAATAATAGAAACTGTTTTGCAAACAAATAAGAGTTTAAAAGATAAGTTAAACCTTCTAATAAAATATGCCAAAAAAAATCTTTCAAAAGACAATATAACCGGAATTCTTGTGGGGTTGAAAAATGAATAA", "aa": "MVVALIFNANGLAYVFNIGDSRLYAYNGLLYQITEDQNYLYQLMREFNLTYEEAALDPNSYKLISCLGPNKKTNCQSFFISQKSAVKYYLLTSDGLHDYVSKPIIETVLQTNKSLKDKLNLLIKYAKKNLSKDNITGILVGLKNE", "aa_hexdigest": "065e93331d98713cea8319b7fb60eda0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "pTC1" ], "truncated": "5-prime", "pseudogene": { "start": 607464, "stop": 608201, "observations": { "mutation": [ 607530, 607677 ], "stop": [ 607530, 607677 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S5", "query_cov": 1.0, "subject_cov": 0.5776892430278885, "identity": 1.0, "score": 284.0, "evalue": 2.29e-95, "gene_start": 1, "gene_end": 145, "reference_start": 107, "reference_end": 251, "reference_sequence": "MVDFCIASDQGKFRKENQDRAIVVNGEYWTLAALCDGMGGHVGGSQCSTLSTNSIKQYFLDTFPQKLECSDKKNVSKWFNNAISFIKEALVNYAKEYTEFEDMGTTMVVALIFNANGLAYVFNIGDSRLYAYNGLLYQITEDQNYLYQLMREFNLTYEEAALDPNSYKLISCLGPNKKTNCQSFFISQKSAVKYYLLTSDGLHDYVSKPIIETVLQTNKSLKDKLNLLIKYAKKNLSKDNITGILVGLKNE" }, "paralog": false, "identity": 0.9918367346938776, "score": 484.0, "evalue": 7.8e-177, "description": "Internal stop codon at 607530, 607677. Nonsense mutation around 607530, 607677.", "psc": { "uniref90_id": "UniRef90_D1J8S5", "gene": "PTC1", "product": "Protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_D1J8S5", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S5", "EC:3.1.3.16", "UniRef:UniRef50_D1J8S5", "COG:COG0631", "COG:T", "KEGG:K20074" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S5" ], "product": "Protein phosphatase" } }, "id": "AHCKFKLKGB_1039", "locus": "GCA013367975_01038" }, { "type": "cds", "sequence": "contig_1", "start": 608194, "stop": 609210, "strand": "+", "frame": 1, "gene": "sPS1", "product": "mitogen-activated protein kinase kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "RefSeq:WP_175933592.1", "SO:0001217", "UniParc:UPI00158F091E", "UniRef:UniRef100_UPI00158F091E", "UniRef:UniRef50_D1J8S6", "UniRef:UniRef90_D1J8S6" ], "nt": "ATGAATAAGATAAAAGAATTACCCGGCATGTTTGTACTTAATAAGTATTTTACTGATTTTGAATTAATTGGGACTGGAGGATTTGGCGAAGTTTATTCTGCTACATATAAAAAAGATAATAAAAGAGTTGCAATTAAAATACTAAATTGTATCGATGATAGCCGCTGGACAGAGCAAAGAACGAGATTCATTAACGAAATAAATATATTAAAGAAAATTCATAGTTCTAATGTAGTAAAGATATTAGGCTGGCACCATAATGGAAACGAAGTTTTTTATGTAATGGAGTTAATTAAAGGCCAAGGTTTAAAAACAATAATTGCAAAAAACAAAAAAATTGATCCGGATACTGCAATTATGTACGCAAAGCAAATATGTCAAGGTTTGATTGAAATCCATGCTGCCAAAATAGTTCATAGAGATTTGAAGCCAAGCAATATAATAATTGACGAAGAAACAAATAGCCTAAAATTAATTGATTTTGGTATTTCAATAGGTGAATCAACTCAGCGTGTAACTAGAAATTATAAAACTGTCGGTTCTGTTCAATATATGGCGCCTGAATTATTGCGGGGAATTGCTGATCCTTCTCCAAGAAGTGATATATATGCATTTGGTATAATTTTGTTTGAAATGCTTGAAGGAAAGGTTCCTTTTAATAATCATGATGATATTAACCAAATTATGATGATGCACATCAATAATGAATTGCCTAGAATGAGTGATGTCAATGTTATTATTCCGCAAGCATTAGAAAATATTGTAATTCGTTGCACTGCAAAATCTGCAGAGCAAAGATATACTGATTGCATTGAACTAATGCATGACTTAGAGACATGTTTAGATTCAAAAAGAGTAAATGAAAAAAGATTGGAATTAAATCCAAGCAAGTCAAAAATGAAAGCCAAAAGAATCTTTAATAGTAAATTATTCAATATTATTTTTTTCACAACAGGAGGCTTTTTAGTAGCCTTTATTGTGATATTCGTGGTTTTATTTTTAAAGGGGATATTGTAG", "aa": "MNKIKELPGMFVLNKYFTDFELIGTGGFGEVYSATYKKDNKRVAIKILNCIDDSRWTEQRTRFINEINILKKIHSSNVVKILGWHHNGNEVFYVMELIKGQGLKTIIAKNKKIDPDTAIMYAKQICQGLIEIHAAKIVHRDLKPSNIIIDEETNSLKLIDFGISIGESTQRVTRNYKTVGSVQYMAPELLRGIADPSPRSDIYAFGIILFEMLEGKVPFNNHDDINQIMMMHINNELPRMSDVNVIIPQALENIVIRCTAKSAEQRYTDCIELMHDLETCLDSKRVNEKRLELNPSKSKMKAKRIFNSKLFNIIFFTTGGFLVAFIVIFVVLFLKGIL", "aa_hexdigest": "f129bce2cf80ca5e19abc46bf3f63840", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158F091E", "ncbi_nrp_id": "WP_175933592.1", "uniref100_id": "UniRef100_UPI00158F091E", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158F091E", "RefSeq:WP_175933592.1", "UniRef:UniRef100_UPI00158F091E" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158F091E", "uniref90_id": "UniRef90_D1J8S6" }, "psc": { "uniref90_id": "UniRef90_D1J8S6", "gene": "SPS1", "product": "mitogen-activated protein kinase kinase", "uniref50_id": "UniRef50_D1J8S6", "cog_id": "COG0515", "cog_category": "T" }, "pscc": { "uniref50_id": "UniRef50_D1J8S6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S6" ], "product": "mitogen-activated protein kinase kinase" }, "genes": [ "sPS1" ], "id": "AHCKFKLKGB_1040", "locus": "GCA013367975_01039" }, { "type": "cds", "sequence": "contig_1", "start": 609211, "stop": 610062, "strand": "+", "frame": 1, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.1.3.100", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022", "KEGG:K06949", "RefSeq:WP_044329804.1", "SO:0001217", "UniParc:UPI0005C96051", "UniRef:UniRef100_UPI0005C96051", "UniRef:UniRef50_D1J8S7", "UniRef:UniRef90_D1J8S7" ], "nt": "ATGATAGAAGGTAAAATAGTTAAATCAATTGCTGGTTTTTATGATATAAAAAGTTTTGAAGATAAACAAATTTATCGTGTAAGAGGAGGTGGCAAACTTCGCTTACTTGATATAGTGCCAATTGTGGGCGACAACGTTTTGTTTAAAAAAGACGAATTCATCTATCAAATTTTAGGTCGCAAGAATTTTTTTATTAGACCTAAAATTGCAAATGTTGATCAAGCAATTGTTGTGATGTCGTTGGTTGAACCTGAATTTAGTTCATTGCTCGTTGATAAATTTTTGATTATTATTGAAAGTAAGAATGTTAAACCTATCATTGTATTAACAAAAAAAGATCTAACTTCTGATTCAAAAATTGCTTTTTATAAAGATCAAGGTTATGAAGTTTTTGAAATTAATTACGAAACAGGCCAAGGATTTGAAGGCTTAGGAGAAATATTTAAACATAAGACAAGCTTCTTTGTTGGTCAAACAGGTGTAGGAAAAACTACCTTGATAAATTATTTGGCAAAAACAAATTTTGAAACGCAAGCAATTTCTAAAGCCTTAAACCGTGGAAAGCATACAACAAGAGAAGTTAGTTTGATTGATTTTAATGGTGGTGAAATAATTGATACACCAGGTTTTTCTTCCATAGAATTTGATTTAACAATTGAAGAACTACCAATTGCTTTTAAATCGTTTAGAGAAGCTGCAAGCAAATGTAAATTTAGAACAAGTTGTAAACATTATTTAGAATCCGTTGAGGATTGTGAAGTTAAAAAATTAGTTTTACAAGGTAAAATTAAACAAGAAAGATATGATAATTACATTAGTTTTTTAAAAAGACTAACAAATCCAATCAAATAG", "aa": "MIEGKIVKSIAGFYDIKSFEDKQIYRVRGGGKLRLLDIVPIVGDNVLFKKDEFIYQILGRKNFFIRPKIANVDQAIVVMSLVEPEFSSLLVDKFLIIIESKNVKPIIVLTKKDLTSDSKIAFYKDQGYEVFEINYETGQGFEGLGEIFKHKTSFFVGQTGVGKTTLINYLAKTNFETQAISKALNRGKHTTREVSLIDFNGGEIIDTPGFSSIEFDLTIEELPIAFKSFREAASKCKFRTSCKHYLESVEDCEVKKLVLQGKIKQERYDNYISFLKRLTNPIK", "aa_hexdigest": "900405c20e342e4b1bdb8634cdb660fc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0005C96051", "ncbi_nrp_id": "WP_044329804.1", "uniref100_id": "UniRef100_UPI0005C96051", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C96051", "RefSeq:WP_044329804.1", "UniRef:UniRef100_UPI0005C96051" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C96051", "uniref90_id": "UniRef90_D1J8S7" }, "psc": { "uniref90_id": "UniRef90_D1J8S7", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.1.3.100", "3.6.1.-" ], "uniref50_id": "UniRef50_D1J8S7", "cog_id": "COG1162", "cog_category": "J", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S7" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" }, "genes": [ "rsgA" ], "id": "AHCKFKLKGB_1041", "locus": "GCA013367975_01040" }, { "type": "cds", "sequence": "contig_1", "start": 610176, "stop": 610718, "strand": "+", "frame": 3, "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "SO:0001217", "UniRef:UniRef50_A0A448ZXM9", "UniRef:UniRef90_A0A454CAB1" ], "nt": "ATGGATGGAATTTTTGTAAACAATACTGCTTTAACATTTCAAGAAATCAAACAAGTTAAAGAAAATTGCAAGAAACATACAATGGATATTCACTTGATGGTTAAAGATGTTTTCAAATATTATGAAATGTACAAGAATATTGGAGATATATTGACATTTCATTATGAAGCGTTTGAAAATAGCGATCTTGAAAAGATGATTAAACAATGCCAAAAAGATAACGTTAAACTTGGCTTAGCAATAAAACCTAATACACAAGTTGAAGAAATAAGACAATATTTAAAACATTGTTCTTTGGTTTTAATAATGAGTGTTGAACCAGGCTTTGGTGGTCAAAAATTTATTGAAAATTCATTTAATAAAATAAAAGAATTAGTAGACATTCGTAAGGCTTTAAATTTAAACTTTATTATTGAAGTGGATGGTGGAGTTAAAGACCAAAATATTGCTAAATGTTTTGAATGTGGCGTTAATTTAGCAGTAGTTGGTTCTTATTTAGTAAAAAACTTTTCAAAAGAAACAATTGACCAACTTTTAAAATAA", "aa": "MDGIFVNNTALTFQEIKQVKENCKKHTMDIHLMVKDVFKYYEMYKNIGDILTFHYEAFENSDLEKMIKQCQKDNVKLGLAIKPNTQVEEIRQYLKHCSLVLIMSVEPGFGGQKFIENSFNKIKELVDIRKALNLNFIIEVDGGVKDQNIAKCFECGVNLAVVGSYLVKNFSKETIDQLLK", "aa_hexdigest": "71ebf2543661b2cd5595913b0ac1b0d5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAB1", "query_cov": 1.0, "subject_cov": 0.8372093023255814, "identity": 0.9890000000000001, "score": 360.0, "evalue": 1.34e-125, "valid": true, "gene": "Rpe", "product": "Ribulose-phosphate 3-epimerase", "uniref50_id": "UniRef50_A0A448ZXM9", "cog_id": "COG0036", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXM9" ], "product": "D-ribulose-5-phosphate 3 epimerase" }, "genes": [ "rpe" ], "id": "AHCKFKLKGB_1042", "locus": "GCA013367975_01041" }, { "type": "cds", "sequence": "contig_1", "start": 610724, "stop": 611593, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S9", "UniRef:UniRef90_D1J8S9" ], "nt": "ATGAAAAAAGCAAATAAAATATTACTGTCTACATTTGCAACTGCAATTTTAGCATTGCCCTTTGCCACAATTTCTTGTAAAGTCGATCAAGTTAAAGAAAAAGAAAAATTAATAAACAATTCTAGTCAGTTAAATAAAGAAAACAAAAAAATATTATTAAATGAATTAAACATATTGAAAACCCGTTTAGTAAATTCAAAACTTAATGAGAAACAAAAAAACGAATTTATTGAAATTCAACTATTTTTAAAATTAATAACACTTAAAGAACTAAAAATATCTAACGAATTATTAAAATATTATAAATTAATGGCCTTGCTTCAAGATAAGTCTGAAGTAAACAAATACGCAGATTTAATCAAAAAATATTCAAAAAAATTTTCTGATGTTTTGAAGGATGATGGTTCAATATTAAATGATATTTACAATCAAGTTGCAAATTTAATTCCTAGAATTAATTTGCTTAAAAATTCTTATATTTTTTCATTAAATATAAATAATAAAAAATTCACTTTTTTAAGTAATAATGGAATACTATTTAATTTAGATTTTATATTGAATAACATCGAAAACAAGGAAAACAAATATTCAATTTATTACAATACAAAGAAATATGAACTAAATTTAGAAATAAAAAACATTGAATTTAATTCAAATATTAAAACTGCATTAGATTTTAAGAATCAAGATTTTTTAACAACTAATTTTTTGGATTATGATAAAGATTTAGTGTGCAATGAAATGTTAAAAAATGCAATGGTCTATAAAAATTATGAACTAGTTGAAGATTTTGATGAAAATGATAAGAACATTAAATTTTCAAAAATGTTTACTAGTCAAGATTTTGTAAATAATTATTTGGTTAATTGA", "aa": "MKKANKILLSTFATAILALPFATISCKVDQVKEKEKLINNSSQLNKENKKILLNELNILKTRLVNSKLNEKQKNEFIEIQLFLKLITLKELKISNELLKYYKLMALLQDKSEVNKYADLIKKYSKKFSDVLKDDGSILNDIYNQVANLIPRINLLKNSYIFSLNINNKKFTFLSNNGILFNLDFILNNIENKENKYSIYYNTKKYELNLEIKNIEFNSNIKTALDFKNQDFLTTNFLDYDKDLVCNEMLKNAMVYKNYELVEDFDENDKNIKFSKMFTSQDFVNNYLVN", "aa_hexdigest": "69f7db74eaf06327283d6fbdc2c9a0a8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 610724, "stop": 611845, "observations": { "mutation": [ 611591 ], "stop": [ 611591 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S9", "query_cov": 1.0, "subject_cov": 0.774798927613941, "identity": 0.99, "score": 534.0, "evalue": 6.28e-190, "gene_start": 1, "gene_end": 289, "reference_start": 1, "reference_end": 289, "reference_sequence": "MKKANKILLSTFATTILALPFATISCKVDQVKEKEKLINNSSQLNKENKKILLNELNILKTRLVNSKLNEKQKNEFIEIQLFLKLITLKELKISNELLKYYKLMALLQDKSEVNKYADLIKKYSKKFSDVLKDDSSILNDIYNQVANLIPRINLLKNSYIFSLNINNKKFTFLSNNGILFNLDFILNNIENKENKYSIYYNTKKYELNLEIKNIEFNSNIKTALDFKNQDFLTTNFLDYDKDLVCNEMLKNAMVYKNYELVEDFNENDKNIKFSKMFTSQDFVNNYLVNWLTNEPNICQIGEDKFLIYYHLPFSIENKKIVQKPSLYISNVVADSTIIKAIASRNVYLLKNLFLLLPKENYNENLKVLNNLMK" }, "paralog": false, "identity": 0.9839142091152815, "score": 717.0, "evalue": 1.01e-264, "description": "Internal stop codon at 611591. Nonsense mutation around 611591.", "psc": { "uniref90_id": "UniRef90_D1J8S9", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J8S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S9", "UniRef:UniRef50_D1J8S9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S9" ], "product": "Lipoprotein" } }, "id": "AHCKFKLKGB_1043", "locus": "GCA013367975_01042" }, { "type": "cds", "sequence": "contig_1", "start": 612239, "stop": 612778, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F067" ], "nt": "ATGTATAAGAAAACAAATAGAAAAATATTGATTTCTCTTGCCATATTATGTGGAGGTTTAGCAGTGGCATCAATTGCTACAGTTGCTGCATATGCAGGTAAATCAAAACCAGAATCAAAAATAAAAAAAGAACATCTTTTGAATGAACTTAAAGGGGCATTAAATCAATTAAAACAATTGATTGCATCACCGGAAGCTAAAAATATTGAAAAATCAATGGAAATCAATATCTTAAATAATGCTAATGTTTCAAAGGATTCCTCAATTAAAGAAATTCAATCTAAAATCAATCAAGTTAAATATGCTATTGATTCATTAAACAAAAAAATCGCAGATCGAAATAAAAAAGAATTTGAAAAATTCAAACAAATCAAAAACGATCTTCAAACTTATATTAATAGAGATTTAAATGTTGTTATATACAATGAAATAACTAAAAAATCTAAAAATGAAATATTGAAATTAAATAGCATATCGCAAAGCTCAAAAGAACAAGAAATTATAGATGCAATAAGCAAATTGTATGATTTAAAGAAATGA", "aa": "MYKKTNRKILISLAILCGGLAVASIATVAAYAGKSKPESKIKKEHLLNELKGALNQLKQLIASPEAKNIEKSMEINILNNANVSKDSSIKEIQSKINQVKYAIDSLNKKIADRNKKEFEKFKQIKNDLQTYINRDLNVVIYNEITKKSKNEILKLNSISQSSKEQEIIDAISKLYDLKK", "aa_hexdigest": "9b39e0dba5c5dfc58975d75a2a3277a8", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001939F067", "query_cov": 1.0, "subject_cov": 0.8731707317073171, "identity": 0.877, "score": 250.0, "evalue": 4.71e-82, "valid": false, "uniref50_id": "UniRef50_UPI001939F067" }, "pscc": { "uniref50_id": "UniRef50_UPI001939F067", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F067" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20221.34980000001, "isoelectric_point": 9.689614295959473 }, "id": "AHCKFKLKGB_1044", "locus": "GCA013367975_01043" }, { "type": "cds", "sequence": "contig_1", "start": 612866, "stop": 613360, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001594B72E", "UniRef:UniRef90_UPI001594B72E" ], "nt": "TTGTTATCAAACAAAATAGTTGAACGTGACTTAGAACTTAAACAAGAAATTTCTAAGGCTGAGGCATCATTAAGTCAAACAAATAAAACTGCATCTAAACTTTTTAGAGAAAGAATATTGCTTCACAAATTTATTAATGAAACTCCATTATTTGAAGAAAAAATGCTTAAAGAAAAAGAAACAAAAATTAACGAGCTTAAGAAACAATCAGAAACTATTTTTGAACAAATTGGAAAAGACCCAAATAATAGCGAAGACTACCATTATAATTACGATTATAATTCCCAATTTTATAGAAACATAATAACTAAAATTGATAGTTTGAACGCTAATGAAAAAAGTTTAACCTTAAATAAAAAGATTGAAGATATAAGCAATGAAATAAATAATCTAGACAAATCATTTTCTAAGTTAGTTTCTGATACCCTTGAAAAATTTGAATATGTAGATGATCTTAAAGACCATATTGATGTTGCAAAAGATCCCAAAAATTGA", "aa": "MLSNKIVERDLELKQEISKAEASLSQTNKTASKLFRERILLHKFINETPLFEEKMLKEKETKINELKKQSETIFEQIGKDPNNSEDYHYNYDYNSQFYRNIITKIDSLNANEKSLTLNKKIEDISNEINNLDKSFSKLVSDTLEKFEYVDDLKDHIDVAKDPKN", "aa_hexdigest": "e5376fd853377682d1b50fb84462d2a3", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 612695, "stop": 613400, "observations": { "insertion": [ 613331 ], "mutation": [ 612776 ], "stop": [ 612776 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001594B72E", "query_cov": 0.9939024390243902, "subject_cov": 0.7117903930131004, "identity": 0.871, "score": 247.0, "evalue": 6.7e-81, "gene_start": 1, "gene_end": 163, "reference_start": 58, "reference_end": 215, "reference_sequence": "MKLNSISQSSKEQEIIDAISKLYDLKKWILAQKLLIDNSINEQKRQLKSLIEIANQILLSNKIVERDLEFKQEISKAEASLSQTNKTTSELFRERILLHKFINETLLLEEKMLKEKETKINELKKQSETIFEHIGKDPNNSIFYYNSQFYRNIITKIDSLNTNEKSLTLNKKIEDISNEINNLDKSFSKLVSDTLEKFEYEDDLKDHMMLQKIPKIELIFKNISDTLGQ" }, "paralog": false, "identity": 0.9145299145299145, "score": 392.0, "evalue": 1.69e-140, "description": "Internal stop codon at 612776. Frameshift due to insertion around 613331. Nonsense mutation around 612776.", "psc": { "uniref90_id": "UniRef90_UPI001594B72E", "uniref50_id": "UniRef50_UPI001594B72E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001594B72E", "UniRef:UniRef50_UPI001594B72E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001594B72E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001594B72E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19325.485899999996, "isoelectric_point": 5.374945259094238 }, "id": "AHCKFKLKGB_1045", "locus": "GCA013367975_01044" }, { "type": "cds", "sequence": "contig_1", "start": 613643, "stop": 613870, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6", "UniRef:UniRef90_UPI00193AA5C6" ], "nt": "ATGCAAGTAGACCAAATAACAGACGAAATGAAAACAAAAATCTTTGATTTGTTAATTAAAGATTTATCTAATATTTTAAAGAAAGATAGTTTTGAAAGCAAACTATTAAGTGCAATATTATTGCCTACAATATTTAAAAAGACATATGAAAATCTTTCTAAAATAATAAAGCCAATTATAGATATGATATATAATAAAGACGCATATAACATAGAAATTATTGGATGA", "aa": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSFESKLLSAILLPTIFKKTYENLSKIIKPIIDMIYNKDAYNIEIIG", "aa_hexdigest": "2d6723254f0e5832865ff9996f363c89", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 613643, "stop": 614173, "observations": { "mutation": [ 613868 ], "stop": [ 613868 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193AA5C6", "query_cov": 1.0, "subject_cov": 0.42134831460674155, "identity": 0.9329999999999999, "score": 132.0, "evalue": 9.91e-38, "gene_start": 1, "gene_end": 75, "reference_start": 1, "reference_end": 75, "reference_sequence": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSFESKLLSAILLPTIFKKPYENLSKIVKPIIDMIHNKDEYSIEIIGWEKASQELSLDISNIKEAEKKALEIKAKLTQENLKNKLKEFKLVKENLGNLIKKASEKDIEVEEDTKFIYENSEEDENSTLYEVISSIIRIKKEIRYLKYKLD" }, "paralog": false, "identity": 0.9431818181818182, "score": 315.0, "evalue": 1.86e-112, "description": "Internal stop codon at 613868. Nonsense mutation around 613868.", "psc": { "uniref90_id": "UniRef90_UPI00193AA5C6", "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193AA5C6", "UniRef:UniRef50_UPI00193AA5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8722.271599999993, "isoelectric_point": 6.168133354187011 }, "id": "AHCKFKLKGB_1046", "locus": "GCA013367975_01045" }, { "type": "cds", "sequence": "contig_1", "start": 613967, "stop": 614179, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATAAATTAAAAGAATTCAAATTAGTAAAAGAAAACTTGGGAAATTTAATTAAAAAACTATCCGAAAAAGGCATCGAAGTCGAAGAAGATACAAAATTCATATATGAAAATTCTGAAGAAGACGAAAATTCTACATTATATGAAGTAATAAATTCTATTATTAGAATAAAAAAAGAAATAAGATACCTTGAATATAAACTAGATTAA", "aa": "MKNKLKEFKLVKENLGNLIKKLSEKGIEVEEDTKFIYENSEEDENSTLYEVINSIIRIKKEIRYLEYKLD", "aa_hexdigest": "800d71b17b9142b0d8b6fa5dbaad40c8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8427.584699999996, "isoelectric_point": 5.275079154968262 }, "id": "AHCKFKLKGB_1047", "locus": "GCA013367975_01046" }, { "type": "cds", "sequence": "contig_1", "start": 614458, "stop": 614787, "strand": "-", "frame": 1, "gene": null, "product": "leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A4608", "UniRef:UniRef90_UPI00280AAAA0" ], "nt": "TTGCCAAAAAATATTGTTAGTTTTGGTCGAGCTATGTTTAATTCAGATTGCAATATAATCTTAGATAGCAATATAACATACATTTCTTGTGGCATAGCAAAAGCCTTTGAAAATAGTAAAAAAGTAATTATTCCAAATTCAGTTAAAGAAATTGAAGAAGGCGCATTTGCGGATTTCGAAAATCTAAGAGAAGTAGTTTTAAATGAAGGAATTGAAAGAATTGGTAGCTATGCTTTTAGAAACCGAAGTCTAGAAGTAGTAACTATTCCAAATTCAGTTAAAGAAATTGGAACTAATGCGTTTTTAGCTGGATTTCAAAATTATTACTGA", "aa": "MPKNIVSFGRAMFNSDCNIILDSNITYISCGIAKAFENSKKVIIPNSVKEIEEGAFADFENLREVVLNEGIERIGSYAFRNRSLEVVTIPNSVKEIGTNAFLAGFQNYY", "aa_hexdigest": "649e5875a0b5a304e710ea4ed5141767", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 614347, "stop": 614901, "observations": { "mutation": [ 614391, 614397, 614460 ], "stop": [ 614391, 614397, 614460 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280AAAA0", "query_cov": 0.981651376146789, "subject_cov": 0.5783783783783784, "identity": 0.804, "score": 97.4, "evalue": 2.6e-23, "gene_start": 1, "gene_end": 107, "reference_start": 39, "reference_end": 145, "reference_sequence": "MEEGAFRNFKELKEVKLNEGLKSIGNYAFLGTSISSVVLPKNIVGFGRDVFNSDCKIILDSNITCISRGIAKAFENSKKVIIPNFVKEIEEGAFEGFENLKEVVLNEGIERIGSYAFRNPRLEVVTIPNSVKEIGTNAFLAGVWNNNVIRSLIPSLKEVYYKGNKNNTPWNWKDIGIDIKIVKEI" }, "paralog": false, "identity": 0.7945945945945946, "score": 203.0, "evalue": 1.05e-67, "description": "Internal stop codon at 614391, 614397, 614460. Nonsense mutation around 614391, 614397, 614460.", "psc": { "uniref90_id": "UniRef90_UPI00280AAAA0", "product": "leucine-rich repeat domain-containing protein", "uniref50_id": "UniRef50_UPI00193A4608", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280AAAA0", "UniRef:UniRef50_UPI00193A4608" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193A4608", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A4608" ], "product": "leucine-rich repeat domain-containing protein" } }, "id": "AHCKFKLKGB_1048", "locus": "GCA013367975_01047" }, { "type": "cds", "sequence": "contig_1", "start": 615341, "stop": 615727, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C8B9B7" ], "nt": "GTGCCTTCAAAAGCATTTTCGCCAATTCTTTCAATTCCATTGCTAAAATTAATCTTCTTTAAATATCAACAATCCTTAAAGGTTTTTGCCTTAATTTCTTTAATTGAACTAGGAATAGAAATTGAAGAGATTTGTGTGCCTTCAAATGCACCTTCTCCAATATTTTCAACACCATTTTTTAAAGTTAATTCCTTTAGATTTTTAAAGTTTTTAAAGGCTTCATTTTCTATTGATTTTACATTTTCAGGAATAGTAACAGAGGCAATTTTTCTAAGTCCCTTTGTTAAATTTTTAGTAATTTTGTCAAAACCATTATCAAATTTTATTTTGCAATTTTCAAAAGAAGCATTTTCATCTATTTCCTCTACCTTTGTGCCTTTAAAATAA", "aa": "MPSKAFSPILSIPLLKLIFFKYQQSLKVFALISLIELGIEIEEICVPSNAPSPIFSTPFFKVNSFRFLKFLKASFSIDFTFSGIVTEAIFLSPFVKFLVILSKPLSNFILQFSKEAFSSISSTFVPLK", "aa_hexdigest": "347ac3c5933d16e5d6aa7c48a1671752", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0005C8B9B7", "query_cov": 0.9453125, "subject_cov": 0.9918032786885246, "identity": 0.7440000000000001, "score": 148.0, "evalue": 9.08e-44, "valid": false, "uniref50_id": "UniRef50_UPI0005C8B9B7" }, "pscc": { "uniref50_id": "UniRef50_UPI0005C8B9B7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C8B9B7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14401.03739999998, "isoelectric_point": 9.56319179534912 }, "id": "AHCKFKLKGB_1049", "locus": "GCA013367975_01048" }, { "type": "cds", "sequence": "contig_1", "start": 615775, "stop": 616047, "strand": "-", "frame": 1, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019398118" ], "nt": "ATGTTATTAACTTTAGGACTTGTTACAACTTCAACAATTTGCTTTCCATTAGTAGCGGCATCATGTACTAAAACAACAGACAGCAATTCAAACAATCAAAATATAGATAATAATCCTTCAAATAATAATGACAACAAACCTAATGACAAAAAGCCAGAATCGTTAAAGAATGAAGATTCTTCTATAAATAACGAAAATCAAAATGTTCAAGATAATAAAGAAAAAGAATTATATGAACTTTTTGATTTTGAAAGAAATTCTTCAAATAATTGA", "aa": "MLLTLGLVTTSTICFPLVAASCTKTTDSNSNNQNIDNNPSNNNDNKPNDKKPESLKNEDSSINNENQNVQDNKEKELYELFDFERNSSNN", "aa_hexdigest": "2915631d8fbe3f88c6ad09f579d75d74", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0019398118", "query_cov": 1.0, "subject_cov": 0.9473684210526315, "identity": 0.789, "score": 76.6, "evalue": 1.81e-16, "valid": false, "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI0019398118" }, "pscc": { "uniref50_id": "UniRef50_UPI0019398118", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019398118" ], "product": "variable surface lipoprotein" }, "genes": [], "id": "AHCKFKLKGB_1050", "locus": "GCA013367975_01049" }, { "type": "cds", "sequence": "contig_1", "start": 616068, "stop": 616196, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATTTAGAAGAATATTACCCAAAAATCCTAGCTAGAACAAGACAAATATTAAAAATAATTTTTGATATATACAAATATGATTCAAAATCAATTTTTAAAGATGACGAAGAAAATAAGGAGAACTAA", "aa": "MHLEEYYPKILARTRQILKIIFDIYKYDSKSIFKDDEENKEN", "aa_hexdigest": "0775fac56697d36591bd1cace06f6afc", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5238.9202000000005, "isoelectric_point": 5.677953910827637 }, "id": "AHCKFKLKGB_1051", "locus": "GCA013367975_01050" }, { "type": "cds", "sequence": "contig_1", "start": 616323, "stop": 616532, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07510.16" ], "nt": "GTGCTTTTTGCAAAAGACGAAATTAAAAAAGATGAATATAAAAAATATATTGATATGATTGGCAATGCAATTAAGTTAGATGACTATTCAAATAAAATTGCTTCTAATAAACGATTAGAAGACAAATATGAAGATTATAAAAATTCAAGCATTGAAAAAAATATTTCTTTTTCAGGCAAAGCTCCATATTTAATAAATATAAAAGAATGA", "aa": "MLFAKDEIKKDEYKKYIDMIGNAIKLDDYSNKIASNKRLEDKYEDYKNSSIEKNISFSGKAPYLINIKE", "aa_hexdigest": "c3b3f26ec90945b5af77b0d02046c70f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07510.16", "name": "GmrSD_C", "length": 54, "aa_cov": 0.782608695652174, "hmm_cov": 1.0, "evalue": 7.690922311263718e-07, "score": 24.422548294067383, "start": 7, "stop": 60 } ], "seq_stats": { "molecular_weight": 8130.173099999998, "isoelectric_point": 7.840145301818849 }, "id": "AHCKFKLKGB_1052", "locus": "GCA013367975_01051" }, { "type": "cds", "sequence": "contig_1", "start": 616638, "stop": 616931, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGAAGATAGTAGCTTTTGAAGGTCAATCATTACGTCCAACAATTTCTGGCATTATAGATAATATCAATAAGAATTCAACTATAGAAACAGCAGATAATTTGAAAGAAGAATTAAAAAGGATAATAAGAGGGCTTAAACAAAGTACTGATTCCGAAATTGATAAATCATTCAATGAAAAATTAAACGGCCACTTAAAAAATCCTGCAAATTTAGAAACAAAAACTAAATCTTTATTACTATCAAGAATTCACTTTTTCTTATCACTTGGTGAATATAAAGATAATAGATGA", "aa": "MWKIVAFEGQSLRPTISGIIDNINKNSTIETADNLKEELKRIIRGLKQSTDSEIDKSFNEKLNGHLKNPANLETKTKSLLLSRIHFFLSLGEYKDNR", "aa_hexdigest": "afba873e4ea82e6f36cd3170a7143da7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11115.560699999984, "isoelectric_point": 9.300031852722167 }, "id": "AHCKFKLKGB_1053", "locus": "GCA013367975_01052" }, { "type": "cds", "sequence": "contig_1", "start": 617034, "stop": 617234, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATGTTTTTAATAAACTTATTAATAAGCTTTACATATATAAATATTTAGAGAATTCTAAAAAATTCCTAAAAGATGATAAAACAAAAAGCAATGTTAATAAATTATCAAGTATTGACATTTTTTCATATTCTATAGTGGCAGAGCAAATTTATGCAGCTACACAAGAAAATACTGTTTTTATTCCTTTGGTATGA", "aa": "MENVFNKLINKLYIYKYLENSKKFLKDDKTKSNVNKLSSIDIFSYSIVAEQIYAATQENTVFIPLV", "aa_hexdigest": "3e78c52e9092ed3faef2cc4b4dca1cdd", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7726.846200000001, "isoelectric_point": 8.892011451721192 }, "id": "AHCKFKLKGB_1054", "locus": "GCA013367975_01053" }, { "type": "cds", "sequence": "contig_1", "start": 617268, "stop": 617975, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03235.19" ], "nt": "ATGTACTCAGATCGAAGCACAAAAATTGACATAGTTGATGGACAACAAAGAAGTTTTACTATTCTTTTAATCTTATTTTGCTTCTTTAAAATATATTGCTACATTTTTTTAAAAGACAAGAAGAAGATTGAAATTAAAGATAAAAACTTATATCTTTTATATAACATATTTGTAAAAAAAGAATATTTAAAGATATACGAAAATATTGACCAACTAGAAGAAGCTAATATATATAAATTTATAAATGAGGTTATTAAGGAAAATCTTAAAATAAATGTTGACGAGGAAAACAAATTCTTTATTAAAACTCTAACAATAATTTTTAATAAACTACCAATAAATATAAAATTTGATGAAATAGCACAAAAAATAGGATTTATCCTAGATGAAGCATACATCACATACACAAACATTAATACAGACTCTTCAGAAATAATTTTTAGAAATCTTAATTTAAATTCAAAACCACTAAGTTCACTAGATTTATTTCGAAATTATTTATATTCTCTAGATAGAGAAAATGAAAAAAACATAAAAACTTTTAATAATGTTTTTTACGATCAAATGGTAAATGAAAGCAATAAAGAAATTAGTGAATCAAAGGTGGAAAATTTTGCATATGTAATAAATAAAATTGATTATTTAATTGAAAGTAATAATCGCACTTCTTTAATAAGCGTCGGAAATTATGAACTTCTAAAAAAATGA", "aa": "MYSDRSTKIDIVDGQQRSFTILLILFCFFKIYCYIFLKDKKKIEIKDKNLYLLYNIFVKKEYLKIYENIDQLEEANIYKFINEVIKENLKINVDEENKFFIKTLTIIFNKLPINIKFDEIAQKIGFILDEAYITYTNINTDSSEIIFRNLNLNSKPLSSLDLFRNYLYSLDRENEKNIKTFNNVFYDQMVNESNKEISESKVENFAYVINKIDYLIESNNRTSLISVGNYELLKK", "aa_hexdigest": "0d3d2f07ef105fbdb8862fbfc0687309", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03235.19", "name": "GmrSD_N", "length": 170, "aa_cov": 0.6978723404255319, "hmm_cov": 0.8529411764705882, "evalue": 3.0969984346501926e-13, "score": 45.59111404418945, "start": 4, "stop": 167 } ], "seq_stats": { "molecular_weight": 28120.057700000045, "isoelectric_point": 5.816470527648925 }, "id": "AHCKFKLKGB_1055", "locus": "GCA013367975_01054" }, { "type": "cds", "sequence": "contig_1", "start": 618075, "stop": 618344, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATATTTTTTGTTCAATAGTAAAACCAATAAAAACGATTTGCAATTTGCCTGATGAAAATATTGAAGAAATTATGGCAATCGAAATTAAAGACGATGATGAGTTTGAAATTAAATTTGAGGAAAATAAACCAAATTCAAATGAAACAAAAAAATTAGTTGAATTAAAAGATAATCAAATATCAAGTCAAGAATTAACAATATATGACTTTTTAAAACGTTTTGCATATGTTCAAATTCCTATATTTCAAAGAAAATATGTATGA", "aa": "MKDIFCSIVKPIKTICNLPDENIEEIMAIEIKDDDEFEIKFEENKPNSNETKKLVELKDNQISSQELTIYDFLKRFAYVQIPIFQRKYV", "aa_hexdigest": "b92a3f4d5e62f1a4e470b9c62bafc760", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10620.118699999994, "isoelectric_point": 4.736416053771973 }, "id": "AHCKFKLKGB_1056", "locus": "GCA013367975_01055" }, { "type": "cds", "sequence": "contig_1", "start": 618387, "stop": 618671, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAACTAAAAAATAAAATGGACATTCTTAGAGAGAAAATGAATATTGAGTCACAAAATTTTGATTACAATGAAGAAGAGCAAAAAGAAAGGCTAATTAATAGCATAAACTCTATTAATGATATTGAAATATCTAATTCGTTTTTTATTTCAATGGATGAATTTTTTAATGACAATGATTACAAAGAAAAAATAAAAAAAGAAATTAAAAAAATTAGTTCTGAATATGTTTCTGCAAAAGGCAACAAATCAAGTTGTAAAACATATTTTGCACCATTATGA", "aa": "MQELKNKMDILREKMNIESQNFDYNEEEQKERLINSINSINDIEISNSFFISMDEFFNDNDYKEKIKKEIKKISSEYVSAKGNKSSCKTYFAPL", "aa_hexdigest": "d306524f296f10bbda4ed0d78a81bee7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11191.469199999987, "isoelectric_point": 5.04539279937744 }, "id": "AHCKFKLKGB_1057", "locus": "GCA013367975_01056" }, { "type": "cds", "sequence": "contig_1", "start": 619274, "stop": 619456, "strand": "-", "frame": 3, "gene": null, "product": "cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D962DD", "UniRef:UniRef90_UPI0034D962DD" ], "nt": "ATGAATAAAAAATATTATGATGAATATAACAAGACTTGCCACCCAGTAAAACATAGATTTTTTGAACAAAAAGAAAGAGGCAGATTAATTGGGGAAGGCACAATATCCGGAACTATTTTTATTATTCAAACATTTAAACCAAAAGTTTGGGTAATAGAAAACCCTAAAACCTCTAAAAGTTGA", "aa": "MNKKYYDEYNKTCHPVKHRFFEQKERGRLIGEGTISGTIFIIQTFKPKVWVIENPKTSKS", "aa_hexdigest": "02004360a6fd0346d09a57af1279fc0b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 619043, "stop": 619495, "observations": { "mutation": [ 619255, 619276, 619465 ], "stop": [ 619255, 619276, 619465 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D962DD", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 128.0, "evalue": 1.09e-36, "gene_start": 1, "gene_end": 60, "reference_start": 14, "reference_end": 73, "reference_sequence": "MLRSISKDGKWVVMNKKYYDEYNKTCHPVKHRFFEQKERGRLIGEGTISGTIFIIQTFKPKVWVIENPKTSKSWEYQRNHWNFEGFENSTYYSSYNNSFSLKPTIFKSNIKLDLLNKRQKGNNDHMARGSYSLRSSIPTQLVADILNQIF" }, "paralog": false, "identity": 0.9133333333333333, "score": 279.0, "evalue": 8.07e-99, "description": "Internal stop codon at 619255, 619276, 619465. Nonsense mutation around 619255, 619276, 619465.", "psc": { "uniref90_id": "UniRef90_UPI0034D962DD", "product": "cytosine methyltransferase", "uniref50_id": "UniRef50_UPI0034D962DD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D962DD", "UniRef:UniRef50_UPI0034D962DD" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D962DD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D962DD" ], "product": "cytosine methyltransferase" } }, "id": "AHCKFKLKGB_1058", "locus": "GCA013367975_01057" }, { "type": "cds", "sequence": "contig_1", "start": 619463, "stop": 619678, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATTCAATTGGAATAAATGACATCTCTTTTAAAGACAAAGAGAGATTTTTTTATCACAAAATTGACCTATCTTTGCAAAACTTTAACTTGATTAAAGAATTAAAGCAACTACCTAAACCTGACATTATTTTAGCATCGCCACCTTGTGAAAGTTGGTCAAGTGCAGATTGTGGCGGAAAAATGCTACGCTCTATTTCCAAAGATGGCAAATGA", "aa": "MHSIGINDISFKDKERFFYHKIDLSLQNFNLIKELKQLPKPDIILASPPCESWSSADCGGKMLRSISKDGK", "aa_hexdigest": "9e56ca5a31521aa043dc74cb859d03a9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8081.286199999997, "isoelectric_point": 8.640133476257326 }, "id": "AHCKFKLKGB_1059", "locus": "GCA013367975_01058" }, { "type": "cds", "sequence": "contig_1", "start": 620126, "stop": 620740, "strand": "+", "frame": 2, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D2IMT5" ], "nt": "ATGGCTAAAGAATTTATCTTAGGTATAGACTTAGGTACTACTAACTCGGTAGTATCTGTTATAGAAAATGGAACCCCAAAAATATTAGAAAATCCAAATGGTAAAAGAACTACACCATCTGTTGTTGCTTTTAAAAATGGAGAAACAATTATTGGTGAAAGTGCTAAAAGACAATTGGAATCAAATAAAGATAGTGTTGCATCTATAAAAAGATTAATGGGTACTTCTCAAACAGTACATCTAAATAATAAAGATTACAAACCTGAAGAAATCAGCGCAATGATTCTAAGTTACATGAAAGATTATGCTGACAAAAAATTAGGTCAACCTGTTAAAAAAGCAGTTATCACAGTCCCAGCATATTTTGACAATGCACAACGTGAAGCAACAAAAAACGCAGGAATTATTGCTGGCCTTGATGTTGTAAGAATTATTAATGAACCTACTGCTGCCGCATTGGCATTTGGATTGAATAAAGATAAAAATGAAAATCAAAAGATCTTAGTATTTGACTTAGGTGGTGGTACATTTGACGTTTCGTTGCTTGAAATGGAAAGTGGAACATTTGAAGTTTTAGCAACAGCAGGCGATAACCACTTAGGTGGTGATGACTGA", "aa": "MAKEFILGIDLGTTNSVVSVIENGTPKILENPNGKRTTPSVVAFKNGETIIGESAKRQLESNKDSVASIKRLMGTSQTVHLNNKDYKPEEISAMILSYMKDYADKKLGQPVKKAVITVPAYFDNAQREATKNAGIIAGLDVVRIINEPTAAALAFGLNKDKNENQKILVFDLGGGTFDVSLLEMESGTFEVLATAGDNHLGGDD", "aa_hexdigest": "8ddb54cc4cdd33d560f0071fd07de60e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002E1EEFC2", "query_cov": 0.9754901960784313, "subject_cov": 0.9660194174757282, "identity": 0.6729999999999999, "score": 264.0, "evalue": 4.31e-87, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_A0A3D2IMT5" }, "pscc": { "uniref50_id": "UniRef50_A0A3D2IMT5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D2IMT5" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "AHCKFKLKGB_1060", "locus": "GCA013367975_01059" }, { "type": "cds", "sequence": "contig_1", "start": 620762, "stop": 621037, "strand": "+", "frame": 2, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9D4" ], "nt": "ATGGTTGAACAAATTAAATCTAAATACAATTTCGATCCAACAACCGACAAAATGGCAATGGCAAGATTAAAAGAAGAAGCAGAACGTGCAAAAATTACTTTATCAGAACAATTAATTGCAAACATTTCTCTTCCATTCTTAGCAATGAATGAAAATGGACCAGTTAATGTTGAATTAGAAATTACACGTGCTACATTTGAATCAATGACTGAACATTTGCTACAAAGAACAAAAAAACCATTATTAGACGTTTTAAATGAAGCAAAATTAACATGA", "aa": "MVEQIKSKYNFDPTTDKMAMARLKEEAERAKITLSEQLIANISLPFLAMNENGPVNVELEITRATFESMTEHLLQRTKKPLLDVLNEAKLT", "aa_hexdigest": "45d398d86ee7045d66076a8f783bff6e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387B89B0", "query_cov": 0.9340659340659341, "subject_cov": 0.8252427184466019, "identity": 0.5760000000000001, "score": 105.0, "evalue": 9.4e-28, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_A0A223M9D4" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9D4" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "AHCKFKLKGB_1061", "locus": "GCA013367975_01060" }, { "type": "cds", "sequence": "contig_1", "start": 621080, "stop": 621913, "strand": "+", "frame": 2, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_Q49539", "UniRef:UniRef90_Q9ZEJ0" ], "nt": "ATGCCAGCTGTTCAAAAACTAGTAGCAGAAGTAACTAATAAAAAACCTAATAATTCAATTAACCCAGATGAAGTAGTTAGCGTTGGAGCTGCTATTCAAGGTGCAATATTAGCAGGAGAAATTCAAGATGTTTTACTACTTGATGTTACTCCTTTAACATTAGGTATTGTAGTAGAAGGTGATGTTGTAGCGCCTTTAATTCCAAGAAATACAACAATTCCTGTTACAAAGAGTCAAATATTCTCAACAGCTGTTGATAATCAAACAGCAGTAACAATTGTTATTACACAAGGTGAAAGACAATTGGCTAGAGATAACAAGATTTTGGGTCAATTTAATCTTGAAGGAATTGAACCTGCACCAAGAGGAATTCCTCAAATTGAAGTAAGTTTCTCAATTGACGTAAATGGTATTACTAAAGTAACAGCAAAAGACAAAAAGACAAACAAAGAACAAACAATCACCATTCAAAACACCTCTTCATTATCAAAAGAAGAAGTAGAAAAGATGGTTAAGGACGCAGAAGCAAATAGAGAAGCCGATCAAAAGAAACGCCATGAAATTGAAGTTATTGTAAAAGCAGAACAATTAAGCAATGACTTAGAAAAGACATTAAAATCAGAACAAGCTAAAAATCTAGGCGAACCTCAAAAACAAGAACTTCAAAAAGAAATAGATGAAATTAAAGAATTAATAAATAAAAAAGATATCGAACAACTAGAAAAGAAAATTACAGAATTTGAACAAAAAATGGCTCAAGCTGCTGAATTTTTAAAGAAACAACAAGGAAATAATAATCCAAACACCAACAACGACAACCCTCAAACTAACTAA", "aa": "MPAVQKLVAEVTNKKPNNSINPDEVVSVGAAIQGAILAGEIQDVLLLDVTPLTLGIVVEGDVVAPLIPRNTTIPVTKSQIFSTAVDNQTAVTIVITQGERQLARDNKILGQFNLEGIEPAPRGIPQIEVSFSIDVNGITKVTAKDKKTNKEQTITIQNTSSLSKEEVEKMVKDAEANREADQKKRHEIEVIVKAEQLSNDLEKTLKSEQAKNLGEPQKQELQKEIDEIKELINKKDIEQLEKKITEFEQKMAQAAEFLKKQQGNNNPNTNNDNPQTN", "aa_hexdigest": "ded9fd313dcfb727cd5128fa41abeb1a", "start_type": "ATG", "rbs_motif": "GGxGG", "psc": { "uniref90_id": "UniRef90_UPI002A7481CD", "query_cov": 0.924187725631769, "subject_cov": 0.8533333333333334, "identity": 0.52, "score": 229.0, "evalue": 8.19e-71, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_Q6L5N6" }, "genes": [ "dnaK" ], "truncated": "5-prime", "pseudogene": { "start": 620780, "stop": 621913, "observations": { "mutation": [ 621035 ], "stop": [ 621035 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZEJ0", "query_cov": 1.0, "subject_cov": 0.46554621848739497, "identity": 1.0, "score": 494.0, "evalue": 5.22e-171, "gene_start": 1, "gene_end": 277, "reference_start": 319, "reference_end": 595, "reference_sequence": "MAKEFILGIDLGTTNSVVSVIENGTPKILENPNGKRTTPSVVAFKNGETIIGESAKRQLESNKDSVASIKRLMGTSQTVHLNNKDYKPEEISAMILSYMKDYADKKLGQPVKKAVITVPAYFDNAQREATKNAGIIAGLDVVRIINEPTAAALAFGLNKDKNENQKILVFDLGGGTFDVSLLEMESGTFEVLATAGDNHLGGDDWDHEIVKWMVEQIKSKYNFDPTTDKMAMARLKEEAERAKITLSEQLIANISLPFLAMNENGPVNVELEITRATFESMTEHLLQRTRKPLLDVLSEAKLTWNDINEVLLVGGSTRMPAVQKLVAEVTNKKPNNSINPDEVVSVGAAIQGAILAGEIQDVLLLDVTPLTLGIVVEGDVVAPLIPRNTTIPVTKSQIFSTAVDNQTAVTIVITQGERQLARDNKILGQFNLEGIEPAPRGIPQIEVSFSIDVNGITKVTAKDKKTNKEQTITIQNTSSLSKEEVEKMVKDAEANREADQKKRHEIEVIVKAEQLSNDLEKTLKSEQAKNLGEPQKQELQKEIDEIKELINKKDIEQLEKKITEFEQKMAQAAEFLKKQQGNNNPNTNNDNPQTN" }, "paralog": false, "identity": 0.9920424403183024, "score": 715.0, "evalue": 1.82e-260, "description": "Internal stop codon at 621035. Nonsense mutation around 621035.", "psc": { "uniref90_id": "UniRef90_Q9ZEJ0", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_Q49539", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZEJ0", "UniRef:UniRef50_Q49539", "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_Q49539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49539" ], "product": "Chaperone protein DnaK" } }, "id": "AHCKFKLKGB_1062", "locus": "GCA013367975_01061" }, { "type": "cds", "sequence": "contig_1", "start": 622135, "stop": 622437, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGACAGCTTTAAACAATTATCACAATTTTTTATTTAAAGTTACTGCTAAGAAAAAAAGCACAATAATTATTCCTATAATTTTATTGTTATGCAGTTTAATTTTGTGTTTTGTTTTTGTTGGTACTAAACCAGCACCGCGTTATTTTAATGTAATAATTTTTGCATATACATTAGTTGCAATATTATTTACAGTATTATATGGCTCTCTCAAGTCCTTAAATATATTTAAAGATCTTGAACAAGATGGGATTGAATTAATTATATTTTCTAAGCCCATTTCAAGAAAAGCAATAATTTGA", "aa": "MKTALNNYHNFLFKVTAKKKSTIIIPIILLLCSLILCFVFVGTKPAPRYFNVIIFAYTLVAILFTVLYGSLKSLNIFKDLEQDGIELIIFSKPISRKAII", "aa_hexdigest": "13c2c510db0c8a1b34ed134faf46f4eb", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11355.716099999987, "isoelectric_point": 9.781288337707515 }, "id": "AHCKFKLKGB_1063", "locus": "GCA013367975_01062" }, { "type": "cds", "sequence": "contig_1", "start": 622480, "stop": 622893, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E50136B", "UniRef:UniRef90_UPI001E50136B" ], "nt": "TTGTTTGCTTTTGTTAGTTCTATTATTGTATATAGTCAAGTTTCAAAAGGAAACATGTTTGGATATTTAGTTTTGTTGGCATTGGTTGCACATTTTCTTGCATATACAATTTTTGGATATATAGCAGCATTGATTGCATATAAAGTTAACCAAAAAATTGCTATAACTGTTCCAATTATAATTTTTGCACCTATGGCAATAGGTGGTGGATTTATATTTGCCAACTCAACATCAACAAATGAAAATTTTGCTCATTATATTAATAGCAAATATAAATACCACAGAGCAGGAAACGAAGTTAATAGTGAAGTATTTTATTTAAATAAAAATGATGATAAATATTATTTAGTTCCAAATGGAATTAATAATAACAAGTTTAGTGATGTACAAAATCAATATTTAAATTTAGCCTGA", "aa": "MFAFVSSIIVYSQVSKGNMFGYLVLLALVAHFLAYTIFGYIAALIAYKVNQKIAITVPIIIFAPMAIGGGFIFANSTSTNENFAHYINSKYKYHRAGNEVNSEVFYLNKNDDKYYLVPNGINNNKFSDVQNQYLNLA", "aa_hexdigest": "1250f1d1d8da8eb3e0a3ac646024dc7c", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 622180, "stop": 623021, "observations": { "deletion": [ 623011 ], "mutation": [ 622435, 622474, 622891, 622936 ], "stop": [ 622435, 622474, 622891, 622936 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E50136B", "query_cov": 1.0, "subject_cov": 0.46440677966101696, "identity": 0.985, "score": 259.0, "evalue": 5.31e-85, "gene_start": 1, "gene_end": 137, "reference_start": 116, "reference_end": 252, "reference_sequence": "MKTALNNYHNFLFKVTAKKKSTIIIPIILLLCSLILCFVFVGTKPAPRYFNVIIFAYTLVAILFTVLYGSLKSLNIFKDLEQDGIELIIFSKPISRKAIIWGKILSFNSLGLIWTLFAFVSSIIVYSQVSKGNMFGYLVLLSLVAHFLAYTIFGYIAALIAYKVNQKIAITVPIIIFAPMAIGGGFIFANSTSTNENFAHYINSKYKYHRAGNEVNSEVFYLNKNDDKYYLVPNGINNNKFSDVQNQYLNLAWKYSNSSANEWQKYSWLAMPYQFVDIFNIENQNIFSNLSSRQY" }, "paralog": false, "identity": 0.9821428571428571, "score": 530.0, "evalue": 3.05e-194, "description": "Internal stop codon at 622435, 622474, 622891, 622936. Frameshift due to deletion around 623011. Nonsense mutation around 622435, 622474, 622891, 622936.", "psc": { "uniref90_id": "UniRef90_UPI001E50136B", "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI001E50136B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E50136B", "UniRef:UniRef50_UPI001E50136B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E50136B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E50136B" ], "product": "ABC transporter permease" } }, "id": "AHCKFKLKGB_1064", "locus": "GCA013367975_01063" }, { "type": "cds", "sequence": "contig_1", "start": 622945, "stop": 623307, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATATCAATTTGTTGATATATTCAACATTGAAAATCAAAATATTTTTAGTAACCTTTCTTCAGACAGTATTAATAATAGTTTAAGTAATTATTTATATTATAAAAATCAAGAAAATACAATATATAAATATAAATTAAATAGAAATTCGGACTTATTAAAATTACCAACATTAGAAAATAAAACTAAGATTAATAAATACTATGTCCCAGGGATGTTAAATTTAAATTCAAATATTGATAATGAAGTAAATAATGGAGTAATTTACGCTAGAAAAAATGCTTCTAATTTTTCTGCTTCTTTTGAAGAAGACAACTTTGTTTATGCATCTCCAAATAATCTAGTAGGAAAATTAAAATGA", "aa": "MPYQFVDIFNIENQNIFSNLSSDSINNSLSNYLYYKNQENTIYKYKLNRNSDLLKLPTLENKTKINKYYVPGMLNLNSNIDNEVNNGVIYARKNASNFSASFEEDNFVYASPNNLVGKLK", "aa_hexdigest": "b6aa917564e8c4b1a5a800e5c70bec1f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13912.351099999982, "isoelectric_point": 7.826155662536623 }, "id": "AHCKFKLKGB_1065", "locus": "GCA013367975_01064" }, { "type": "cds", "sequence": "contig_1", "start": 623314, "stop": 623901, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5A3247", "UniRef:UniRef90_UPI001E5A3247" ], "nt": "ATGAGCCAAGTTTTAAAATATCATAAATTCACAGAAGCAGCCGAAGAATTTTATTCTAAGGTTAAAAATGAAATTAGTTTAAAGAAATTAGTTAAACCACATGATATTAAAAAGTTATTGTTAGAAACGATTTCAAATGAAGTTAATAATACAAATAGTAAGTTCTTTAATATAGATAATGTTAACTTAGCAGTATTCGACCCTCATGGTGTTAGAGATGGAAGATTACAATCTGAAACAGAAAGAAAAATTTATATTGCCACAGCATTGCTATATTTTATTTATTTTAAAGATAATCAAGGTCAAGTTCTTAAATCATTATTGAAATCTGAAAGCGATAAAACAACATATGCACCAAGCCAAATAGAAATCAAGGTAGATGGTTATATCTATGAAATAGGTGGATATGAAAGCTACGAACCAAAACAACAAGTGCAAGAAAATAAAATAACTAAAAATAAACAAGTAGTAGTTAGATATGAATTAATTCCAAGCAATAAAAATTATTTATTCCAAACAGTAGATGATGTATATTCAATTGAAAGAGATAGAAAAATAGTTAATAAACAATTTTATATTTTAATTTGA", "aa": "MSQVLKYHKFTEAAEEFYSKVKNEISLKKLVKPHDIKKLLLETISNEVNNTNSKFFNIDNVNLAVFDPHGVRDGRLQSETERKIYIATALLYFIYFKDNQGQVLKSLLKSESDKTTYAPSQIEIKVDGYIYEIGGYESYEPKQQVQENKITKNKQVVVRYELIPSNKNYLFQTVDDVYSIERDRKIVNKQFYILI", "aa_hexdigest": "d810305dfafd60b10a78998ab3a592d7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 623014, "stop": 623967, "observations": { "mutation": [ 623305, 623899 ], "stop": [ 623305, 623899 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E5A3247", "query_cov": 1.0, "subject_cov": 0.5571428571428572, "identity": 0.9790000000000001, "score": 369.0, "evalue": 1.71e-126, "gene_start": 1, "gene_end": 195, "reference_start": 134, "reference_end": 328, "reference_sequence": "MNDKNIHDLQCHINLLIYSTLKIKIFLVTFLRDNINNSLSNYLYYKNQENTIYKYKLNRNSDLLKLPTLENKTKINKYYVPGMLNLNSNIDNEVNNGVIYARKNASNFSASFEEDNFVYASPNNLVGKLKWKIMSQVLKYHKFIEAAEEFYSKVKNEISLKKLVKPYDIKKLLLEMISNEVNNTNSKFFNIDNVNLAVFDPHGVRDGRLQSETERKIYIATALLYFIYFKDNQGQVIKSLLKSESDKTTYAPSQIEIKVDGYIYEIGGYESYEPKQQVQENKITKNKQVVVRYELIPSNKNYLFQTVDDVYSIERDRKIVNKQFYILIWIVVAIILMMANRFLYLRKDYK" }, "paralog": false, "identity": 0.9747634069400631, "score": 611.0, "evalue": 1.17e-224, "description": "Internal stop codon at 623305, 623899. Nonsense mutation around 623305, 623899.", "psc": { "uniref90_id": "UniRef90_UPI001E5A3247", "uniref50_id": "UniRef50_UPI001E5A3247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E5A3247", "UniRef:UniRef50_UPI001E5A3247" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E5A3247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5A3247" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 22894.838900000006, "isoelectric_point": 8.76139850616455 }, "id": "AHCKFKLKGB_1066", "locus": "GCA013367975_01065" }, { "type": "cds", "sequence": "contig_1", "start": 623967, "stop": 624671, "strand": "+", "frame": 3, "gene": "rbbA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1131", "COG:JV", "RefSeq:WP_020002508.1", "SO:0001217", "UniParc:UPI000365E072", "UniRef:UniRef100_A0A454CAA1", "UniRef:UniRef50_A0A449B6T2", "UniRef:UniRef90_D1J8T4" ], "nt": "ATGGATAATCATATTTTAGAATTAAAAAATGTAGTAAAAATCTATAATAAATCAGATCGTGGAATTCATAATATTTCATTTGCTGTCGAATCTGGAAATTTTCATGCATTTATTGGCGAAAATGGTGCAGGAAAAACAACAACAATTAAAACAATAATTGGTTCTTATATAAATTTTAAAGGTGAAATACTTATTGGTGGAATTACAAACTTAAATCCTAATTCTAAAAAGATTCTTGGTTATGTTCCAGAAAATGCAGTGTTTCCTAAAGAACTAACAACATATGAATATTTAAAGGCCCTTGCATTATTATCGGGGATAAATAAAACCGAAGTAAAGGCAAAAATCGAAAGTTACTTACAAAGATTTGGAATTATTGAATTAAAAAACGAAAAACCCGCTAATTTTTCATCAGGTCAAAAGAAAAAAGTTTTATTAATACAGGCTTTGATTAACGAGCCTAAATTATTGATTTTAGATGAACCTGCTGCTAATTTAGACCCTACTGCAAGATTTGAATTGTTTTCAATATTACATGAACTAAATGAAAAAGGAACAACAATTTTTATTAGTTCCCACGTTCTAAGTGAAATTGATAAATATGCAAATTCGTTGACATTAATTCATGGCGGAAAGATTTTATATTCTGGCCCTAAATATGATTCATTGGAAAAAATATTTTATGAAAAAGTTATTAAAAATTAG", "aa": "MDNHILELKNVVKIYNKSDRGIHNISFAVESGNFHAFIGENGAGKTTTIKTIIGSYINFKGEILIGGITNLNPNSKKILGYVPENAVFPKELTTYEYLKALALLSGINKTEVKAKIESYLQRFGIIELKNEKPANFSSGQKKKVLLIQALINEPKLLILDEPAANLDPTARFELFSILHELNEKGTTIFISSHVLSEIDKYANSLTLIHGGKILYSGPKYDSLEKIFYEKVIKN", "aa_hexdigest": "cbe68b00230783e80869ce26faa8a7ac", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000365E072", "ncbi_nrp_id": "WP_020002508.1", "uniref100_id": "UniRef100_A0A454CAA1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000365E072", "RefSeq:WP_020002508.1", "UniRef:UniRef100_A0A454CAA1" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CAA1", "uniref90_id": "UniRef90_D1J8T4" }, "psc": { "uniref90_id": "UniRef90_D1J8T4", "gene": "RbbA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A449B6T2", "cog_id": "COG1131", "cog_category": "JV" }, "pscc": { "uniref50_id": "UniRef50_A0A449B6T2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B6T2" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "rbbA" ], "id": "AHCKFKLKGB_1067", "locus": "GCA013367975_01066" }, { "type": "cds", "sequence": "contig_1", "start": 624649, "stop": 625041, "strand": "+", "frame": 1, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A573B35", "UniRef:UniRef90_UPI000A573B35" ], "nt": "ATGAAAAAGTTATTAAAAATTAGTTTGTCATTGCCATTAGTTATTGCAACAATTCCATTAGTTGCTTCAAGCTGCAAACAAACAAATATTGAAATTAAAAAACCAGATGATAAAAAAGAAACAGCAAATCAAATCCAAGCAAAAATTAATAAAGAATTTCAAAAAAATAAAGTGGTTCAAAAATTACTAAGTTCATTTATTGAAAATGAAAAAGAACAAGATATTTTTGTACAAAACCAAAATAATATATTGCCCAATGTATATTCAGAACTAAAGTTTTCTTTGTTGTATTTCCCTATTTATAATAGAGCCTCAGATAATCCGGGTAGCGAACTTTATGGATTTATAAGACAATCAAGAGAAATTATTGAAAACACATTGCAAAGAAATTGA", "aa": "MKKLLKISLSLPLVIATIPLVASSCKQTNIEIKKPDDKKETANQIQAKINKEFQKNKVVQKLLSSFIENEKEQDIFVQNQNNILPNVYSELKFSLLYFPIYNRASDNPGSELYGFIRQSREIIENTLQRN", "aa_hexdigest": "bf92365f4129e4aec179e2b3aa3b5ef9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 624649, "stop": 625174, "observations": { "insertion": [ 625123 ], "mutation": [ 625039, 625045 ], "stop": [ 625039, 625045 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A573B35", "query_cov": 1.0, "subject_cov": 0.7471264367816092, "identity": 1.0, "score": 243.0, "evalue": 9.04e-81, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MKKLLKISLSLPLVIATIPLVASSCKQTNIEIKKPDDKKETANQIQAKINKEFQKNKVVQKLLSSFIENEKEQDIFVQNQNNILPNVYSELKFSLLYFPIYNRASDNPGSELYGFIRQSREIIENTLQRNWYWYLKQIGNFVYSINPYNDRYIETSQTKKQFNLPKKTLVFYIK" }, "paralog": false, "identity": 0.9770114942528736, "score": 328.0, "evalue": 1.18e-116, "description": "Internal stop codon at 625039, 625045. Frameshift due to insertion around 625123. Nonsense mutation around 625039, 625045.", "psc": { "uniref90_id": "UniRef90_UPI000A573B35", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI000A573B35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A573B35", "UniRef:UniRef50_UPI000A573B35" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A573B35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A573B35" ], "product": "aromatic motif membrane protein" } }, "id": "AHCKFKLKGB_1068", "locus": "GCA013367975_01067" }, { "type": "cds", "sequence": "contig_1", "start": 625183, "stop": 625578, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE", "UniRef:UniRef90_UPI000A5C6EDE" ], "nt": "ATGCCTAAAGATATTATTGAATTGCCTTATAACCCAATAAGTTCAGATGAATTAAAAGAAATAGTCGCTAAAAAAATTAATGAAAGTATTGATTCTGAAGTTCAAAACCAATTTAGCGATTATTTAGAAGATAGAATTAATGATTCATCAAAGTTAAAAATAGATACAGCAACAGATATAACCAATTTTTTAAAAAGCAAAATTAACCTAAATGATGCAAAAAAGCTAATAGAAAATTATTTGAACAATAAGAACAAATTTGAAATTAGTAATCAATATCAAATTAATAATGAAATTTCTAAAAAAATATTTAATTTATTAAATACAAAAGGATCTTCATTTGATAACTATTCCTCAAAGAAAATCTTCTACGTAGCGTACGACCACAAAACTTGA", "aa": "MPKDIIELPYNPISSDELKEIVAKKINESIDSEVQNQFSDYLEDRINDSSKLKIDTATDITNFLKSKINLNDAKKLIENYLNNKNKFEISNQYQINNEISKKIFNLLNTKGSSFDNYSSKKIFYVAYDHKT", "aa_hexdigest": "9e4e1c4cff5ed0a2b02a5d7614dd0895", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 625183, "stop": 625881, "observations": { "mutation": [ 625576 ], "stop": [ 625576 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "query_cov": 1.0, "subject_cov": 0.5282258064516129, "identity": 0.924, "score": 231.0, "evalue": 5.62e-75, "gene_start": 1, "gene_end": 131, "reference_start": 1, "reference_end": 131, "reference_sequence": "MPKDIIELPYNPISSDELKEIVAKKINESIDSKVQNQFSDYLEDRIDESSKLKIDINSEITNFLRNKINLNDAKKLIENYLNNKNKFEISNEYQINNEISKKIFNLLNTKGSSFDNYSSKKIFYVAYDHKTWIRVIVYTENNVKKISINPDVYILNQSNSYEQSKEILKKLNKQIFIERLNSIIDSLIENINEKDFNIKNFFEKNNDKKFFELFQKFSYNKYLIEAMQKINKEKDLIYRYSWETVDYE" }, "paralog": false, "identity": 0.9439655172413793, "score": 434.0, "evalue": 1.78e-157, "description": "Internal stop codon at 625576. Nonsense mutation around 625576.", "psc": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A5C6EDE", "UniRef:UniRef50_UPI000A5C6EDE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15251.942099999984, "isoelectric_point": 5.864840507507323 }, "id": "AHCKFKLKGB_1069", "locus": "GCA013367975_01068" }, { "type": "cds", "sequence": "contig_1", "start": 625588, "stop": 625929, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE", "UniRef:UniRef90_UPI000A5C6EDE" ], "nt": "ATGGTTTATACTGAAAATAACGTTAAAAAAATTTCTATAAATCCTGATGTTTATATTTTAAATCAAAGCAATAGCTATGAACAAAGCAAAGAAATTTTAAAAAAATTAAATAAACAAATTTTTATTGAACGGTTGAATTCAATTATTGATTCTTTAATTGAAGACATAAATGAAAAAGATTTCAATATTAAAAACTTTTTTGAAAAAAATAATGATAAAAAATTCTTTGAATTGTTTCAAAAGTTTTCATACAATAAATACTTGATTGAAGCAATGCAAAAAATTAATAAAGAAAAAGATCTAATTTATCGCTATAGTTGGGAGACAGTAGATTATGAATAA", "aa": "MVYTENNVKKISINPDVYILNQSNSYEQSKEILKKLNKQIFIERLNSIIDSLIEDINEKDFNIKNFFEKNNDKKFFELFQKFSYNKYLIEAMQKINKEKDLIYRYSWETVDYE", "aa_hexdigest": "be6fbaba476b63547b4a3348655918a7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 625288, "stop": 625929, "observations": { "mutation": [ 625576 ], "stop": [ 625576 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "query_cov": 1.0, "subject_cov": 0.45564516129032256, "identity": 0.982, "score": 214.0, "evalue": 1.3e-68, "gene_start": 1, "gene_end": 113, "reference_start": 136, "reference_end": 248, "reference_sequence": "MPKDIIELPYNPISSDELKEIVAKKINESIDSKVQNQFSDYLEDRIDESSKLKIDINSEITNFLRNKINLNDAKKLIENYLNNKNKFEISNEYQINNEISKKIFNLLNTKGSSFDNYSSKKIFYVAYDHKTWIRVIVYTENNVKKISINPDVYILNQSNSYEQSKEILKKLNKQIFIERLNSIIDSLIENINEKDFNIKNFFEKNNDKKFFELFQKFSYNKYLIEAMQKINKEKDLIYRYSWETVDYE" }, "paralog": false, "identity": 0.9436619718309859, "score": 404.0, "evalue": 9.42e-146, "description": "Internal stop codon at 625576. Nonsense mutation around 625576.", "psc": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A5C6EDE", "UniRef:UniRef50_UPI000A5C6EDE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13858.594099999984, "isoelectric_point": 5.629015541076659 }, "id": "AHCKFKLKGB_1070", "locus": "GCA013367975_01069" }, { "type": "cds", "sequence": "contig_1", "start": 625922, "stop": 626227, "strand": "+", "frame": 2, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241181B1", "UniRef:UniRef90_UPI00241181B1" ], "nt": "ATGAATAAAAGAAATTTATTAAATTTTTTGTTTGTTCCCACTTTAATTCTTGCACCATTAACTGCTATATCGTGCGTTGATAAAAGTGAAAAAAGCGATTTGAATGCATTAGCAACTAAAATAGGAGATGTTGTAAACAAAAAAGAAAATCCTGCTATTGAAGTAACCAAAAAAATAATTAACGACTTATTAAAACAAGTATATAGGGATAATGATGTTGAAAAAATTAATTTTTTAGATCAACAACAAAATAAAGAATATCAAAAAAACATTTTTTTGAAAATTAAAGAACTTTCAAAAAAATGA", "aa": "MNKRNLLNFLFVPTLILAPLTAISCVDKSEKSDLNALATKIGDVVNKKENPAIEVTKKIINDLLKQVYRDNDVEKINFLDQQQNKEYQKNIFLKIKELSKK", "aa_hexdigest": "670d981eccc8d6508d2c5805232c3907", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 625922, "stop": 626530, "observations": { "mutation": [ 626225, 626297, 626348 ], "stop": [ 626225, 626297, 626348 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00241181B1", "query_cov": 1.0, "subject_cov": 0.4855769230769231, "identity": 1.0, "score": 185.0, "evalue": 1.13e-57, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MNKRNLLNFLFVPTLILAPLTAISCVDKSEKSDLNALATKIGDVVNKKENPAIEVTKKIINDLLKQVYRDNDVEKINFLDQQQNKEYQKNIFLKIKELSKKWNSKLNNTEKENIANQLNQIYEKNWYIVLTNINKFNLRFYEWLLFDKPEFLSQIRADGKHSKEYLNSLKSEKKPDNVPIINNYLRELKEGDESGELSNMNMLYLKKR" }, "paralog": false, "identity": 0.9702970297029703, "score": 383.0, "evalue": 2.36e-138, "description": "Internal stop codon at 626225, 626297, 626348. Nonsense mutation around 626225, 626297, 626348.", "psc": { "uniref90_id": "UniRef90_UPI00241181B1", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI00241181B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00241181B1", "UniRef:UniRef50_UPI00241181B1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00241181B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241181B1" ], "product": "aromatic motif membrane protein" } }, "id": "AHCKFKLKGB_1071", "locus": "GCA013367975_01070" }, { "type": "cds", "sequence": "contig_1", "start": 626354, "stop": 626617, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATAAGCCTGAATTTTTAAGCCAAATTAGGGCAGATGGAAAGCATAGCAAAGAATATTTAAATTCATTAAAAAGCGAAAAGAAACCAGATAATGTTCCGATTATTAATAATTATTTAAAGGAATTAAAAGAAGGTGATGAATCAGGAGAACTTTCAAATATGAACATGCTTTATTTAAAAAAAGATAGTATGATATTCAGATTTAGAGTTGTTGATACATTGACAAAACCAAAAATCAAACTAACGTGTTTTGTGTGA", "aa": "MFDKPEFLSQIRADGKHSKEYLNSLKSEKKPDNVPIINNYLKELKEGDESGELSNMNMLYLKKDSMIFRFRVVDTLTKPKIKLTCFV", "aa_hexdigest": "c03b67fc41a90cbb416fdcf9376ff96d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10187.76769999999, "isoelectric_point": 9.01450138092041 }, "id": "AHCKFKLKGB_1072", "locus": "GCA013367975_01071" }, { "type": "cds", "sequence": "contig_1", "start": 626651, "stop": 626815, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCAGATATTGTTCATAGCGCTTATATTCACGGCGTTCAAGAAGGATTCAATAGATTTGAAAATGATATGGCAAATCGTTATGGCAAACCTGCCAAAATGATTATGGGAATTTTCGATGATAATGCTTCTAGCGCCAATGGAGGAAAAGAAAATGAATAA", "aa": "MISDIVHSAYIHGVQEGFNRFENDMANRYGKPAKMIMGIFDDNASSANGGKENE", "aa_hexdigest": "6afceb24d95b2ddc41a50030defe4c38", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5978.556100000002, "isoelectric_point": 5.140711784362792 }, "id": "AHCKFKLKGB_1073", "locus": "GCA013367975_01072" }, { "type": "cds", "sequence": "contig_1", "start": 626772, "stop": 626966, "strand": "+", "frame": 3, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E405388", "UniRef:UniRef90_UPI001E405388" ], "nt": "ATGATAATGCTTCTAGCGCCAATGGAGGAAAAGAAAATGAATAAAAAAATTTTGATGACTCTATCATTGAGCACGTTAAGCATTATACCTAGCTGTTTTTCTTTAATTTCATGTACTAAAGAAAATAAACAAGATGAAAAAAAATCATTGAATCAAATAATTGAGGGCAACGAAAATGCAAACGAAAGAGAATGA", "aa": "MIMLLAPMEEKKMNKKILMTLSLSTLSIIPSCFSLISCTKENKQDEKKSLNQIIEGNENANERE", "aa_hexdigest": "be39ced1aba265aec6a6b11b80823f19", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 626808, "stop": 627062, "observations": { "mutation": [ 626964 ], "stop": [ 626964 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E405388", "query_cov": 0.8125, "subject_cov": 0.6046511627906976, "identity": 1.0, "score": 96.3, "evalue": 8.78e-25, "gene_start": 13, "gene_end": 64, "reference_start": 1, "reference_end": 52, "reference_sequence": "MNKKILMTLSLSTLSIIPSCFSLISCTKENKQDEKKSLNQIIEGNENANEREWKNFLNRDYVKEILRLSFDADVDAKNKYIAEQKN" }, "paralog": false, "identity": 0.9647058823529412, "score": 156.0, "evalue": 2.84e-51, "description": "Internal stop codon at 626964. Nonsense mutation around 626964.", "psc": { "uniref90_id": "UniRef90_UPI001E405388", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI001E405388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E405388", "UniRef:UniRef50_UPI001E405388" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E405388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E405388" ], "product": "aromatic motif membrane protein" } }, "id": "AHCKFKLKGB_1074", "locus": "GCA013367975_01073" }, { "type": "cds", "sequence": "contig_1", "start": 627279, "stop": 627485, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTGAATCATCAGATGAACAATCAGCATTAACTGCGCAAGGTAATTCCTTGATTTTAGGCCATTTTTATAAACCAAGGAGTAATGAAATTATTCAATATACAACTCAAGTTACAAAAAATGACAAAAATGGTGAGATAACTTATGATGTTTATTTATTAACAAAAGAAGGTTTTATTTTTCAATTAACTATTGATTGA", "aa": "MNKFESSDEQSALTAQGNSLILGHFYKPRSNEIIQYTTQVTKNDKNGEITYDVYLLTKEGFIFQLTID", "aa_hexdigest": "33eab71dfe66f98f3d3e9cfc57e2b537", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7808.6076, "isoelectric_point": 4.907330894470214 }, "id": "AHCKFKLKGB_1075", "locus": "GCA013367975_01074" }, { "type": "cds", "sequence": "contig_1", "start": 628029, "stop": 628196, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAATAATAGGAAGAACAACAAAAAAACAAAGGAAAATTATGTCAGAACAAAAACAACAAGCACAACCAGCAGTAGCTCCAACACAAGCAGAACCTGCTATTGTATCACGCGAAAAATTATTAGAAGCAGGAACATACTTTGGACACAGAGTAAGCCAATGA", "aa": "MLEIIGRTTKKQRKIMSEQKQQAQPAVAPTQAEPAIVSREKLLEAGTYFGHRVSQ", "aa_hexdigest": "7461acaa8e4de47d38c08816005aa396", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6180.082899999999, "isoelectric_point": 9.989134407043458 }, "id": "AHCKFKLKGB_1076", "locus": "GCA013367975_01075" }, { "type": "cds", "sequence": "contig_1", "start": 628233, "stop": 628334, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTCATATTATTGATATTGCCAAAACTCAAAAAACATTGGAATATGCTTACAAACTATTAAATAAAATGGCTCAAAAACCAATTTCATTTATTTGA", "aa": "MGIHIIDIAKTQKTLEYAYKLLNKMAQKPISFI", "aa_hexdigest": "82e2324bb86e16d48affdc20ce88ce1b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3821.5944, "isoelectric_point": 9.602904319763184 }, "id": "AHCKFKLKGB_1077", "locus": "GCA013367975_01076" }, { "type": "cds", "sequence": "contig_1", "start": 628533, "stop": 628973, "strand": "+", "frame": 3, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "COG:COG0052", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02967", "SO:0001217", "UniRef:UniRef50_D5E5E7", "UniRef:UniRef90_D1J8T8" ], "nt": "ATGGACAAGAAAATTGCTAAATTGCAAAAGAACTTAGGTGGTATTAGAAAATTAGCTGGAAAAGCAGCAATGCCACAAGTAATGATTTGTGCATCACCTATTGATGATGAAATAGCAATTAGAGAAGCAAAAAAGAAAGGTTTGAAAATTTTTGCAATTCAAGATACAAACTCAAACCCAGATTTAGTTGACTTCGTAATACCAGCAAACGATGATTCTGTAAAGTCAATTACTTTAATTACAACTATCTTGGCAGACGCAATAGCATCAGCACATGGTGAAAAACCAGTATATGCATATAAGTCAAATGAAGAAATTATCTTGCCAGAAGAACAAAAACCAGAAAAGGTTGAAAAAATAGCAAACACCGAAGAAAGAAAACCAAGAACTTTTGTTAAAAAAGCTAACGTTGAATCAGAAAATACTAAGAAATCTGAATAA", "aa": "MDKKIAKLQKNLGGIRKLAGKAAMPQVMICASPIDDEIAIREAKKKGLKIFAIQDTNSNPDLVDFVIPANDDSVKSITLITTILADAIASAHGEKPVYAYKSNEEIILPEEQKPEKVEKIANTEERKPRTFVKKANVESENTKKSE", "aa_hexdigest": "3faafe1bfebc59bb844b5f86d14e5d0f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "rpsB" ], "truncated": "5-prime", "pseudogene": { "start": 628233, "stop": 628973, "observations": { "mutation": [ 628332, 628413 ], "stop": [ 628332, 628413 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8T8", "query_cov": 1.0, "subject_cov": 0.48344370860927155, "identity": 1.0, "score": 244.0, "evalue": 3.93e-79, "gene_start": 1, "gene_end": 146, "reference_start": 157, "reference_end": 302, "reference_sequence": "MSEQKQQAQPAQPAVAPTQAEPAIVSREKLLEAGTYFGHRVSQWNPKMKQYIWGKRMGIHIIDIAKTQKTLEYAYKLLNKMAQKPISFIWVGTKKQAKKAIESAAARTNSVYVSERWLGGTLTNSQTIFRSVRELERLEELQKSEYQGYTKKEGLLMDKKIAKLQKNLGGIRKLAGKAAMPQVMICASPIDDEIAIREAKKKGLKIFAIQDTNSNPDLVDFVIPANDDSVKSITLITTILADAIASAHGEKPVYAYKSNEEIILPEEQKPEKVEKIANTEERKPRTFVKKANVESENTKKSE" }, "paralog": false, "identity": 0.991869918699187, "score": 433.0, "evalue": 8.9e-156, "description": "Internal stop codon at 628332, 628413. Nonsense mutation around 628332, 628413.", "psc": { "uniref90_id": "UniRef90_D1J8T8", "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_D5E5E7", "cog_id": "COG0052", "cog_category": "J", "kegg_orthology_id": "K02967", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8T8", "UniRef:UniRef50_D5E5E7", "COG:COG0052", "COG:J", "KEGG:K02967", "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5E7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5E7" ], "product": "Small ribosomal subunit protein uS2" } }, "id": "AHCKFKLKGB_1078", "locus": "GCA013367975_01077" }, { "type": "cds", "sequence": "contig_1", "start": 628994, "stop": 629110, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGTAGATTTAAAAAAAATTAAGGAATTAAGAGAAAGAACAAATTCAGGATTTTTAGATGTTAAAAATGCATTAGAAGCAACAAACAACGATATTGAAAAAGCAATTGAATGA", "aa": "MSVDLKKIKELRERTNSGFLDVKNALEATNNDIEKAIE", "aa_hexdigest": "70bdda38816f371176ed85c51c179563", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4333.8732, "isoelectric_point": 5.289686775207519 }, "id": "AHCKFKLKGB_1079", "locus": "GCA013367975_01078" }, { "type": "cds", "sequence": "contig_1", "start": 629111, "stop": 629875, "strand": "+", "frame": 2, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0006414", "SO:0001217", "UniRef:UniRef50_Q98Q37", "UniRef:UniRef90_D1J8T9" ], "nt": "TTGCAAGAAAAAGGTATAGTTAAGGCTGCTAAAAAAGCCGGAAGAATTGCAGCAGAAGGAATAGTAAGAGCTGTTATTAAAGATAATATTGCTGTGATTTTTGAGTTAAATTCAGAAACAGATTTTGTTGCTAAAAATCAATTATTTTTAGACTTTGCTAAATTATTAGAAGAAGAATTGCCAAAAGTTAATTTCTCAAATATCGATGATTTATTAGAACAAAAAATTAATGGTAAAACAATTGAAGAATATACAACAGATTTAACAGCAAAAATTGGCGAAAAGATTCATTTAAGAAGAGCATTAAAGTACGAAGCAAAAGAAGATGAAGTTGTTGCTGGATATACACATGCTAACAATAGAATTGCTACTATTTTAATCGCTAAAGGACATAACCAAGAAGCATTAAGACATGTTGCAATGCATATTGCAGCCTTAAACCCTGCTCATTTATTCGAAACATGTTTGGATGAAGAAGAATTAAAGGAAATTTATGAACGTATTAACAACGATCCTAAATTAGCATCTAAACCAGAAAAAATTCAAGTTTCTATGAAATCAGGAATGTTAAGAAAAGAATTCAATGAACGTGGCGTTTTAATGTATCAACCATTTGTTATGGAAGAATCAAAAACAGTTCAACAATATTTAACAGAAAATAAATTACAATTAATAGATGCAACAAGATTTGAAGTAGGCGAAGGAATAGAAAAGAAAACTGTTGATTTTGCTACAGAAGTTGCTGAACAAATGTCACTTAAATAA", "aa": "MQEKGIVKAAKKAGRIAAEGIVRAVIKDNIAVIFELNSETDFVAKNQLFLDFAKLLEEELPKVNFSNIDDLLEQKINGKTIEEYTTDLTAKIGEKIHLRRALKYEAKEDEVVAGYTHANNRIATILIAKGHNQEALRHVAMHIAALNPAHLFETCLDEEELKEIYERINNDPKLASKPEKIQVSMKSGMLRKEFNERGVLMYQPFVMEESKTVQQYLTENKLQLIDATRFEVGEGIEKKTVDFATEVAEQMSLK", "aa_hexdigest": "3438d9ef9e9f6effb134627f2937a747", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8T9", "query_cov": 1.0, "subject_cov": 0.8668941979522184, "identity": 0.992, "score": 476.0, "evalue": 8.46e-169, "valid": true, "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_Q98Q37", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q37" ], "product": "Elongation factor Ts" }, "genes": [ "tsf" ], "id": "AHCKFKLKGB_1080", "locus": "GCA013367975_01079" }, { "type": "cds", "sequence": "contig_1", "start": 629907, "stop": 630734, "strand": "-", "frame": 2, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "RefSeq:WP_175933602.1", "SO:0001217", "UniParc:UPI00158C0805", "UniRef:UniRef100_UPI00158C0805", "UniRef:UniRef50_D1J8U0", "UniRef:UniRef90_D1J8U0" ], "nt": "ATGGAGAATAAATCTTTTAGTCAACTAGTAAAAGAAGAAATTATGTCTTTTCAGTTAAAAAAACAAGAAAAGTTTTTTTTGATGGCTGGCATAATTTATTGTTGTAATGTAAAAAACAATGAAGCTAAATTAGTAATAAATAATATTAAGATTAGGACATATATATTAAATATTTGTAATGAACTAAAAATTTCTTATACGTATACTAAAAAAAATGAAATTTCAATAAATACAAATTTTTTAGCTAACATAAGCGTTAAATATCAAGGAAAATTCTTTGCTGGTTTATTCCTTTCTTCTGGTTCAATAATAAATGGAAGAACAAATCATCTAGAGTTAAAATTTAATGATTTAATAAATGCAGAAAATGCTTTATCAATTTTAAATTCTCACGGATTAGAATTTAAAAAAATAATTAGAAAACAAAAAATAATAATTTATTTAAAAAAAATTGAAAATATTTGTGATTTTTTAAAAGCTATTGAAACCATTGAATCCTATTATTTATTCGAAGATGGCAAAATTCAGAGAGATTTTTATAATTCAGCAAATAGAATAACAAATCTCGACGTATATAATCAAGAACGTATTGCAAAAGCAAATTTAGAATTTATTAAAAATTATCAATTTATAAAAGAAAACAATCTCTTGGATGAGTTTAGCGATGAAGAACTTGCGCTATTTGAATTAAAATTAAATAGTTTAGACAGTTCCTTAAATGAACTTAGTTTATTATTAGAAACAAAAAACATTAAAAAGTCTCGTTCTGCATTGAATCATTTCTTAATTAAACTAAAAAAAATAACTAAAAAGTATCAAAAAAAATAG", "aa": "MENKSFSQLVKEEIMSFQLKKQEKFFLMAGIIYCCNVKNNEAKLVINNIKIRTYILNICNELKISYTYTKKNEISINTNFLANISVKYQGKFFAGLFLSSGSIINGRTNHLELKFNDLINAENALSILNSHGLEFKKIIRKQKIIIYLKKIENICDFLKAIETIESYYLFEDGKIQRDFYNSANRITNLDVYNQERIAKANLEFIKNYQFIKENNLLDEFSDEELALFELKLNSLDSSLNELSLLLETKNIKKSRSALNHFLIKLKKITKKYQKK", "aa_hexdigest": "73541e399da8ccb12ec02b4261730c59", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00158C0805", "ncbi_nrp_id": "WP_175933602.1", "uniref100_id": "UniRef100_UPI00158C0805", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C0805", "RefSeq:WP_175933602.1", "UniRef:UniRef100_UPI00158C0805" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C0805", "uniref90_id": "UniRef90_D1J8U0" }, "psc": { "uniref90_id": "UniRef90_D1J8U0", "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_D1J8U0", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U0" ], "product": "DNA-binding protein WhiA" }, "genes": [ "whiA" ], "id": "AHCKFKLKGB_1081", "locus": "GCA013367975_01080" }, { "type": "cds", "sequence": "contig_1", "start": 630783, "stop": 631310, "strand": "-", "frame": 2, "gene": "pncA", "product": "Isochorismatase-like domain-containing protein", "db_xrefs": [ "COG:COG1335", "COG:HR", "RefSeq:WP_175933603.1", "SO:0001217", "UniParc:UPI00158AAC16", "UniRef:UniRef100_UPI00158AAC16", "UniRef:UniRef50_A0A077L748", "UniRef:UniRef90_D1J8U1" ], "nt": "ATGAAAGCAATCTTTGTTGTTGATATGGTTAATGGATTTTGTAAAAAAGGTGCACTATATAGTTGCAATATTGAGTCAATAATTGTTCCTATAAAAAATTTTTTAGAAACTCAATATAAAGACAATGATATATATTTTTTAAACGATGCTCATAGTAGCGATGATATTGAAATGCAATCATACCCAATACATTGTTTAAAAAATAGCAAAGAAAGTCAAGTAGTAGATGAATTAAAAAAATATGCTAAAAATATAATTGAAAAGAATTCTACTAACTCCTTTTTTGCATTAAAAAAGGAAATTTTAAGCAAGTATGATTCCTTTGAAATTATTGGTTGTTGTTCAGATATTTGCATTTTACAATTTGCAATAACTCTAAAAACTTATTTCAATCATTTAAAGCAAAATAAAGAAATTATTGTCTTTAAAAATTTAATTGCAACATTTAATATTCCAAATCATAATTCTCAAGAATATCACGATTTTGCTTTAAATTTAATGGCTAATGCAGGCATAAAAATTAAATAA", "aa": "MKAIFVVDMVNGFCKKGALYSCNIESIIVPIKNFLETQYKDNDIYFLNDAHSSDDIEMQSYPIHCLKNSKESQVVDELKKYAKNIIEKNSTNSFFALKKEILSKYDSFEIIGCCSDICILQFAITLKTYFNHLKQNKEIIVFKNLIATFNIPNHNSQEYHDFALNLMANAGIKIK", "aa_hexdigest": "2bafd65631ea9168f44f5b99d9e4dfc5", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158AAC16", "ncbi_nrp_id": "WP_175933603.1", "uniref100_id": "UniRef100_UPI00158AAC16", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158AAC16", "RefSeq:WP_175933603.1", "UniRef:UniRef100_UPI00158AAC16" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158AAC16", "uniref90_id": "UniRef90_D1J8U1" }, "psc": { "uniref90_id": "UniRef90_D1J8U1", "gene": "PncA", "product": "Isochorismatase-like domain-containing protein", "uniref50_id": "UniRef50_A0A077L748", "cog_id": "COG1335", "cog_category": "HR" }, "pscc": { "uniref50_id": "UniRef50_A0A077L748", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L748" ], "product": "Isochorismatase-like domain-containing protein" }, "genes": [ "pncA" ], "id": "AHCKFKLKGB_1082", "locus": "GCA013367975_01081" }, { "type": "cds", "sequence": "contig_1", "start": 631480, "stop": 631632, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCGAAGAACAATTAGTTGTTACTTCAAGTCCTAAGAAAAAGATTGGCTTCTTTTCAGCTATTCTTATCGTTATGGGTTCGGCTATCGGTGTTGGGGTTTTCCTACGTTCAAAGAACGTTTTACAAAACTCAGCTAACAACGTCGTATGA", "aa": "MAEEQLVVTSSPKKKIGFFSAILIVMGSAIGVGVFLRSKNVLQNSANNVV", "aa_hexdigest": "02596721837798f5e42380334bb00f3d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5293.2089000000005, "isoelectric_point": 9.996161460876465 }, "id": "AHCKFKLKGB_1083", "locus": "GCA013367975_01082" }, { "type": "cds", "sequence": "contig_1", "start": 632278, "stop": 632433, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACACCCAGAAAAAACTCCTGCTGCTATTGTTATTGGTTTAGCATCAATGACTGTTCTATACATGTTAATTGCTGTTACAATGACATTAGGTGGAAATAACGGTTCATTCTACGGATTCGGAGATGAATTAAACGCTGTTAAAGCAGGTTGA", "aa": "MKHPEKTPAAIVIGLASMTVLYMLIAVTMTLGGNNGSFYGFGDELNAVKAG", "aa_hexdigest": "9b2780453f473bbd2db75d02ed91c875", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5301.184600000001, "isoelectric_point": 6.514055442810059 }, "id": "AHCKFKLKGB_1084", "locus": "GCA013367975_01083" }, { "type": "cds", "sequence": "contig_1", "start": 632635, "stop": 632748, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATCTTCACTGCTATTGGTGCTTACGCATACTTTGGTGGAGGATATTCAACAGACTACGGTTACCAAATTGATAACCTATTAACATTTGCTGACTTGATGGCAAACTGA", "aa": "MVIFTAIGAYAYFGGGYSTDYGYQIDNLLTFADLMAN", "aa_hexdigest": "80ebdad5c03bf7b06982cc073cecfbe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4048.5029000000013, "isoelectric_point": 4.0500284194946286 }, "id": "AHCKFKLKGB_1085", "locus": "GCA013367975_01084" }, { "type": "cds", "sequence": "contig_1", "start": 632890, "stop": 633135, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTCTTAGCAATTGACCCATTCATATCAATAGGTCTATCAGCAGCACAATACGTTGCAGATAAAACAGAAGACACCAGAAACGGCTTAGTAGGAAACATTGCTACATCAATTCTATTTATAATATATGCAGCAGTTTCATTCTTAGGCGCTCCAGTTGAAAGACAAATTGCTAAATCTAACAAGATTAAATTTGATAGAGTTCTTTCAGGAAACATTTGTGCACACGAAGTTCCAGAATGA", "aa": "MFFLAIDPFISIGLSAAQYVADKTEDTRNGLVGNIATSILFIIYAAVSFLGAPVERQIAKSNKIKFDRVLSGNICAHEVPE", "aa_hexdigest": "9c1fc98d61c8346b14bf1c75121b9444", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8788.067599999997, "isoelectric_point": 5.7058618545532225 }, "id": "AHCKFKLKGB_1086", "locus": "GCA013367975_01085" }, { "type": "cds", "sequence": "contig_1", "start": 633336, "stop": 633602, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "ATGAAAAATAGTAAAGAACAGATTAACAATATTCCTTATGTTTTAAATGAAACATTGATTAACACAAATTTAAAAACTGGCCTAAGTTCACAAGAGGCTGATTTAAGACTTAAAAAGTTTGGAAAAAATGAATTGCATAAAGCAAAAAAAATAAATCCATTTTTAATATATTTAGAGCAATTTAAAGATATATTGGTCATTATATTATTATTTGCAGCAATAATATCTTATGCTCTTGCGATTGTTTCAGGAACTCAAAATAATTGA", "aa": "MKNSKEQINNIPYVLNETLINTNLKTGLSSQEADLRLKKFGKNELHKAKKINPFLIYLEQFKDILVIILLFAAIISYALAIVSGTQNN", "aa_hexdigest": "be4a572fcc7d7732a074669c22cdccb2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 56, "aa_cov": 0.6363636363636364, "hmm_cov": 1.0, "evalue": 2.67862007608281e-21, "score": 70.39323425292969, "start": 21, "stop": 76 } ], "seq_stats": { "molecular_weight": 9976.59089999999, "isoelectric_point": 9.477255439758302 }, "id": "AHCKFKLKGB_1087", "locus": "GCA013367975_01086" }, { "type": "cds", "sequence": "contig_1", "start": 633732, "stop": 634241, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCAAATTAAGTCCCTTACAAGCTAAAGTCGTCCGTGATGGCAATTTAATAACGTTAGCATCAAGTGATATAACCGTTGGCGATATTGTTTTAATTGAATCAGGTGATGTTGTACCTTCTGATGGTTATTTGTTGTTTAGTTCAAATTTAACTGTTATTGAGAGTGCATTAACCGGGGAAAGTGAACCGGCGACAAAAGATTACCAAGCTAAAAGAAATTTAGCATTAGCAATGGCAGATAGACAATTTATGGTTTATTCTTCGACTGTGGTTGCTACAGGAACAGCTTATTTTGTTGTTACATCGGTAGGTGAAAATAACGAAATCGGAAAAATTTCTAAACTAGTTAATCAACAAAAAGAATCGATGACTCCTCTTCAAATTAAAATAAATAAATTAGGAAAAATATTTGGATATTCAGGTCTTGCATTATTTTTAGCAAGTTTAGTGACTCAAATAGTTTTCCATGCGGTATCAAGAAATTCTTTTGCTGATACAATGTTTTGA", "aa": "MSKLSPLQAKVVRDGNLITLASSDITVGDIVLIESGDVVPSDGYLLFSSNLTVIESALTGESEPATKDYQAKRNLALAMADRQFMVYSSTVVATGTAYFVVTSVGENNEIGKISKLVNQQKESMTPLQIKINKLGKIFGYSGLALFLASLVTQIVFHAVSRNSFADTMF", "aa_hexdigest": "967030df51c550dd9b8fd29e713174b5", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18190.707799999993, "isoelectric_point": 5.791972923278809 }, "id": "AHCKFKLKGB_1088", "locus": "GCA013367975_01087" }, { "type": "cds", "sequence": "contig_1", "start": 634187, "stop": 634327, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAATAATTGAAGTAAAAGCAATTAACCCCTCAGGAATGGCAGCAACGGCTAGTGAAATGGCTCCAACTATTGCATTGCCTCAAAACATTGTATCAGCAAAAGAATTTCTTGATACCGCATGGAAAACTATTTGA", "aa": "MAKIIEVKAINPSGMAATASEMAPTIALPQNIVSAKEFLDTAWKTI", "aa_hexdigest": "214ebe2f35348b699851dc003f7ae15a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4873.708299999999, "isoelectric_point": 6.013644981384277 }, "id": "AHCKFKLKGB_1089", "locus": "GCA013367975_01088" }, { "type": "cds", "sequence": "contig_1", "start": 634341, "stop": 634751, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAGAAAGAATGCAATTGTTAAGAACTTAATGGCTGTTGAGTCTCTTGGCAATTGTGCAGTAATATGTTCAGATAAAACAGGAACATTAACACAAAACAAGATGACAATTGTAGATGCTTTTGATGGAAGCCAACTACTATCAAATAATAGTGAAATTAAAAACTTCTTAAATATTATAGAATTAGGTTCATTGTGTTGCGATGCAAATTTAACTCGCAATGAAAAAGATGAATATGTTGTAGTTGGAGATCCAACAGAAATAGCATTTTTATATGAACTTGAAAAATTCTCTAATTTTAAATACAAAAACAACCTAGAAATAGAATATAAGCGTTTGCATTTGTTCCCATTTGATTCAGATAGAAAAATGAAATCAGTAATAAATCAAAACAACAACAATTATTGA", "aa": "MARKNAIVKNLMAVESLGNCAVICSDKTGTLTQNKMTIVDAFDGSQLLSNNSEIKNFLNIIELGSLCCDANLTRNEKDEYVVVGDPTEIAFLYELEKFSNFKYKNNLEIEYKRLHLFPFDSDRKMKSVINQNNNNY", "aa_hexdigest": "237d367c7ca5e2e9f74400b822ecfd33", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15514.500899999985, "isoelectric_point": 5.440651130676269 }, "id": "AHCKFKLKGB_1090", "locus": "GCA013367975_01089" }, { "type": "cds", "sequence": "contig_1", "start": 634761, "stop": 635252, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGGGAGCTCCTGAAATAATAATTGAAAAATGCAATTTTGATTCGCCTTCATTAAAAGAAAAATATTTACAAATAAATAATCAATGGGCAAATAATGCTTATAGAGTCTTAGCAATAGCAAGAAAATCCTTAAAAAAGGAAACAATTGAGAAATTCAATGAATCATCAATTTCTAAATTACAAGAGCATGTAGAAAATGATTTAGAAATTGTTGGATTAGTTGCAATGATTGATCCTCCAAGAGAAAGTGCAAAAGATGCTATTACATTATGCAAACAAGCAGGAATAAAACCTGTTATGATTACTGGTGATAATTTAAATACAGCAAAAGCAATAGCAACAAATCTTGGCATATATAGTGATCAAGATCGTGCTATAACAGGAACTGAACTTTCAAACATTTCTGACGAAGAATTAGTTCAAAATATTGAAAAATACAGTGTTTATGCTAGAGTTAAACCAGAAGATAAATTAAGAATTGTTAATGCCTGA", "aa": "MGAPEIIIEKCNFDSPSLKEKYLQINNQWANNAYRVLAIARKSLKKETIEKFNESSISKLQEHVENDLEIVGLVAMIDPPRESAKDAITLCKQAGIKPVMITGDNLNTAKAIATNLGIYSDQDRAITGTELSNISDEELVQNIEKYSVYARVKPEDKLRIVNA", "aa_hexdigest": "d8e8a92940c2d3589194f1b47045d34b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 137, "aa_cov": 0.8220858895705522, "hmm_cov": 0.8686131386861314, "evalue": 3.25073492640895e-12, "score": 42.520755767822266, "start": 28, "stop": 161 } ], "seq_stats": { "molecular_weight": 18192.565899999994, "isoelectric_point": 5.331520271301269 }, "id": "AHCKFKLKGB_1091", "locus": "GCA013367975_01090" }, { "type": "cds", "sequence": "contig_1", "start": 635277, "stop": 635555, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGGTGATGGTGTTAATGATGCTCCAGCATTGAAAGCTTCAGATATAGGCTGCGCTATGGGCATAACAGGAACTGAAGCTTCAAAACAAGCTGCAAATGTTATATTGGCTGATGATAATTTTGCCACCATAGTTTCAGCAGTAGCAAACGGAAGATCAATTTATCAAAAAATTAAAAACGTAATTCAAAGTTTATTGATTACATCGATTGCAGAGATCATTTTATTATTTATAGGTTTGTTTTTGTTTAAAGCGATTTTTTCAAGTGCTGAATGA", "aa": "MTGDGVNDAPALKASDIGCAMGITGTEASKQAANVILADDNFATIVSAVANGRSIYQKIKNVIQSLLITSIAEIILLFIGLFLFKAIFSSAE", "aa_hexdigest": "d5e62797fa58ea977c0fde073636f9ca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9562.986599999993, "isoelectric_point": 4.842193412780761 }, "id": "AHCKFKLKGB_1092", "locus": "GCA013367975_01091" }, { "type": "cds", "sequence": "contig_1", "start": 635757, "stop": 635951, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGGAATACTATCGTTAATAACTTATTATTTAGCAGCGTTATATGGCAGAAATAATGGATTTAATGCAGAAGATATATTAAAAGCAGCATCTACTTGTTCATTTTTGGTTTTAGGTATATCTGCTACATTCAATTCTATAAACTTAATGACAAAGAAATCAATCTTTATATCAAATCCATTATATTATTGA", "aa": "MIGILSLITYYLAALYGRNNGFNAEDILKAASTCSFLVLGISATFNSINLMTKKSIFISNPLYY", "aa_hexdigest": "b373f778aac643a913b5d13a3787bb7d", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7046.185699999998, "isoelectric_point": 8.889303779602052 }, "id": "AHCKFKLKGB_1093", "locus": "GCA013367975_01092" }, { "type": "cds", "sequence": "contig_1", "start": 635991, "stop": 636230, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGTAGTATTTGTAAAAAATATAAATCTAGTGTTTGGATGTTACACCGAAATTCATTCTAAGCCCGAACTTTTAGCACTAGGATTAGCATTACCATTAGTAATTATTCCAATATATTCAATTCATAAATTAATCATATATTTAATTGAAAAAAACAAAATTGAAGAAAATAAAATTGTTGAATTTAAAATGATATTACCTCCAAGGCAAGCCAAACGAAACCAAAAAAGACATTAA", "aa": "MIVVFVKNINLVFGCYTEIHSKPELLALGLALPLVIIPIYSIHKLIIYLIEKNKIEENKIVEFKMILPPRQAKRNQKRH", "aa_hexdigest": "3c6e14d2256edf972d652d8277613857", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9235.153899999988, "isoelectric_point": 9.722557640075681 }, "id": "AHCKFKLKGB_1094", "locus": "GCA013367975_01093" }, { "type": "cds", "sequence": "contig_1", "start": 636311, "stop": 636676, "strand": "-", "frame": 3, "gene": null, "product": "Zinc-ribbon domain-containing protein", "db_xrefs": [ "RefSeq:WP_175933606.1", "SO:0001217", "UniParc:UPI00158B92D5", "UniRef:UniRef100_UPI00158B92D5", "UniRef:UniRef50_D1J8U5", "UniRef:UniRef90_A0A454CAA9" ], "nt": "ATGAATTATTGCCCAAATTGCGGCACTAAATTAAATAACGCTAATTATTGCCCAAATTGCGGAAAAAAGGTGGAAAAAGATGCATATGTAACAACACAAATGAATACAATGCAACCATCTCAGCCTTATTTGCAACCTCAACAACAAATCCAAAATTCAGTAACTAATGAATTTGCTATTGTTGGATTTATATTGTCATTTTTCTTTTCAATATTAGGTCTTATTTTTAGTTGTATTGGACTTAGCAAGTCAAAATCAATGAATGGTTCAGGAAAAGCTTTGTCAATAGCGGGAATTTTTATTTCTTGCTTTACTATATTTTTTGCCTTTGTTGTATTCATTACTTTAATAGGCGTAAGCCTTTAA", "aa": "MNYCPNCGTKLNNANYCPNCGKKVEKDAYVTTQMNTMQPSQPYLQPQQQIQNSVTNEFAIVGFILSFFFSILGLIFSCIGLSKSKSMNGSGKALSIAGIFISCFTIFFAFVVFITLIGVSL", "aa_hexdigest": "a1b6007247a34d73df502207bc182b00", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00158B92D5", "ncbi_nrp_id": "WP_175933606.1", "uniref100_id": "UniRef100_UPI00158B92D5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B92D5", "RefSeq:WP_175933606.1", "UniRef:UniRef100_UPI00158B92D5" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B92D5", "uniref90_id": "UniRef90_A0A454CAA9" }, "psc": { "uniref90_id": "UniRef90_A0A454CAA9", "product": "Zinc-ribbon domain-containing protein", "uniref50_id": "UniRef50_D1J8U5" }, "pscc": { "uniref50_id": "UniRef50_D1J8U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U5" ], "product": "Zinc-ribbon domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_1095", "locus": "GCA013367975_01094" }, { "type": "cds", "sequence": "contig_1", "start": 636794, "stop": 636886, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATGTAAGGAAATGCACTTTATGCAACTTGACGGCGAAACTTTCGATATAGAAAATAAAATATATATAAACAAAAAAAGAATCAATTAA", "aa": "MECKEMHFMQLDGETFDIENKIYINKKRIN", "aa_hexdigest": "792aa9df189a1e6b36362e2805f492ff", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3719.2922000000017, "isoelectric_point": 5.596105766296386 }, "id": "AHCKFKLKGB_1096", "locus": "GCA013367975_01095" }, { "type": "cds", "sequence": "contig_1", "start": 637088, "stop": 637678, "strand": "-", "frame": 3, "gene": "lCB5", "product": "Transcriptional regulator", "db_xrefs": [ "COG:COG1597", "COG:IR", "SO:0001217", "UniRef:UniRef50_D1J8U6", "UniRef:UniRef90_D1J8U6" ], "nt": "ATGCTATATATATTTTATAATTCTCTATCAAAGAGTGGAAAATGTCAAAGAAAGATGTATGACATAGTTTCTGACGCCATTAAAGTATTTTTACAACCTGAGGTAAAAATATTTGACATTATAAAAATTAAAGACAGTCAAAATCTAGTTTCTCAAATGGATATAGAAAAAGATATTGTTTTATTAATCGGCGGTGATGGAACATTAACCAATATCACTAATAAAATTCACAATATAAAACCTTTACCCAAAATTTATGCATATAAAGCAGGAACTGGTAATGATTTTCTAAGGTCTTTAAAAATAAAACAAGATGTTCAAATAATACATAAAAAATATTATTTGATATCTCCTTACATTACCAACTTACCAACGATTAAATTTAAAAACGACCAAGATGAAAGATCTTTTTTAAATGGCATTGGTTTTGGTATTGATGCAGTTATTGCAAAAAAAGTTAATGATGAAAAAATGCTTAAAAATAAATCGTCATTTGCTAAAGTAACAATAAATACATTTAAGACATTCAAAACAATTCCTTTAATAAAAATTAATGTAGATGGAAAAGAATTAGAATTTAAAAATGTTTGA", "aa": "MLYIFYNSLSKSGKCQRKMYDIVSDAIKVFLQPEVKIFDIIKIKDSQNLVSQMDIEKDIVLLIGGDGTLTNITNKIHNIKPLPKIYAYKAGTGNDFLRSLKIKQDVQIIHKKYYLISPYITNLPTIKFKNDQDERSFLNGIGFGIDAVIAKKVNDEKMLKNKSSFAKVTINTFKTFKTIPLIKINVDGKELEFKNV", "aa_hexdigest": "97ab0a59badab95f59dff4a963c1436b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "lCB5" ], "truncated": "5-prime", "pseudogene": { "start": 636786, "stop": 637678, "observations": { "deletion": [ 636808 ], "mutation": [ 636967, 636979, 637090 ], "stop": [ 636967, 636979, 637090 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8U6", "query_cov": 1.0, "subject_cov": 0.6068111455108359, "identity": 0.99, "score": 382.0, "evalue": 4.76e-132, "gene_start": 1, "gene_end": 196, "reference_start": 1, "reference_end": 196, "reference_sequence": "MLYIFYNSLSKSGKCQRKMYDIVSDAIKVFLQPEVKIFDIIKIKDSQNLVSQMDIEKDIVLLIGGDGTLTNITNKIHNIKPLPKIYAYKAGTGNDFLRSLKIKQDVQIIHKKYYLISPYIANLPTIKFKNDQDERSFLNGIGFGIDAVIAKKVNDEKMLKNKSSFAKVTINAFKTFKTIPLIKINVDGKELEFKNVWLASIMNNCYFGGGMKLAPNCNNTDKLNLIIINNVSKWKLAWLFFSVYSGKHLRYKKYVHEIQGYKFSMECKEMHFMQLDGETFDIENKIYINKKKNQLNKKRPNKNWDVFLLLYCVIFIYKINLKK" }, "paralog": false, "identity": 0.9764309764309764, "score": 574.0, "evalue": 2.64e-210, "description": "Internal stop codon at 636967, 636979, 637090. Frameshift due to deletion around 636808. Nonsense mutation around 636967, 636979, 637090.", "psc": { "uniref90_id": "UniRef90_D1J8U6", "gene": "LCB5", "product": "Transcriptional regulator", "uniref50_id": "UniRef50_D1J8U6", "cog_id": "COG1597", "cog_category": "IR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8U6", "UniRef:UniRef50_D1J8U6", "COG:COG1597", "COG:IR" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U6" ], "product": "Transcriptional regulator" } }, "id": "AHCKFKLKGB_1097", "locus": "GCA013367975_01096" }, { "type": "cds", "sequence": "contig_1", "start": 637682, "stop": 638428, "strand": "-", "frame": 3, "gene": "argE", "product": "Xaa-His dipeptidase", "db_xrefs": [ "COG:COG0624", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZY24", "UniRef:UniRef90_D1J8U7" ], "nt": "ATGATCTATGTAACAGAAATTCATTTTCCAAAGGTTCCAGGAGTTGAATTAAATGGGGGCGACGTTGTTAACCAAATTCCTGATTCAACATCTTTAGTATTGAATGAAAAACCTAATATTATTAAGGGGATTGGTGGACACGGCTCAACACCTGAAAAAGGTGACAATGCAATAATTAAAGCCATTAATATAGCAAGCAAAGAAAATAAAGAATTTGCTAAATCTAAATTATTTAAATTTATTAAAGAAAATTTAAATAGCAAATCATTTAATTTAAGTAATATTTTTAAAAACTATAGTGATTTTTCGGGCGAATTATCTGCAAATTTAGGTATTATTAGAACCTTGAAAAATGAATATGTTTTAACTTTTGATTTTAGAGTCCCTGTTTCTCATAAAATTGATGATGTAACTAAAAGCTTAAATGATTATTTAAAAGATGAATTTGGCGATGATGTATTTTCAAAATTGCAAGGGTTCAAGGAACCAAAGTATATTGAACAAGATTCTCCAATTGTAAGAATTCTTATGGATACATATAATGAAGCGATGAATTCAAATGAAAAACCTTTAGCAATTGGCGGCGGTACATTTGCAAGATTAATAAAAACTTGTGTCGCTTTTGGTTCAACTAAATATATGCATTTAATGCATGGGCCAAACGAATACTTTACATTTGATGAAATAAAATCATCACTTGAAATTTATATCAATGCATTAAATAGATTACAAGATTTGGAAAAATAA", "aa": "MIYVTEIHFPKVPGVELNGGDVVNQIPDSTSLVLNEKPNIIKGIGGHGSTPEKGDNAIIKAINIASKENKEFAKSKLFKFIKENLNSKSFNLSNIFKNYSDFSGELSANLGIIRTLKNEYVLTFDFRVPVSHKIDDVTKSLNDYLKDEFGDDVFSKLQGFKEPKYIEQDSPIVRILMDTYNEAMNSNEKPLAIGGGTFARLIKTCVAFGSTKYMHLMHGPNEYFTFDEIKSSLEIYINALNRLQDLEK", "aa_hexdigest": "5a54aa0d90e982c4bb7cec90702eb2d2", "start_type": "ATG", "rbs_motif": null, "genes": [ "argE" ], "truncated": "3-prime", "pseudogene": { "start": 637682, "stop": 638728, "observations": { "mutation": [ 638452, 638563, 638701 ], "stop": [ 638452, 638563, 638701 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8U7", "query_cov": 1.0, "subject_cov": 0.5662100456621004, "identity": 0.992, "score": 485.0, "evalue": 3.62e-170, "gene_start": 1, "gene_end": 248, "reference_start": 191, "reference_end": 438, "reference_sequence": "MKKFITYKQSKEDFDEMIKNIANICAIPSISEEDFSSEFPFGKETDNALNYALNLAKSFGFNIYKDPKNRYGFAEFGNGDKIIGILAHLDVVPAGDESQWRTSAFVPVITNESIIGRGSLDDKGPAIINLYAMKYIHDHGLLSNEWKIRIIFGISEETTMKSMKSYLADFKDPYISYTPDGEWPLIYAEKMIYVTEIHFPKVPGVELNGGDVVNQIPDSTSLVLNEKPNIIKGIGGHGSTPEKGDNAIIKAINIASKENKEFAKSKLFKFIKENLNSKSFNLSNIFKNYSDFSGELSANLGIIRTLKNEYVLTFDFRVPVSHKIDDVTKSLNDYLKDEFGNDVFSKLQGFKEPKYIEQDSPIVRILMDTYNEAMNSNEKPLAIGGGTFARLIKTCVAFGSTKYMHLMHGPNEYFTFDEIKSSLEIYINALNRLQDLEE" }, "paralog": false, "identity": 0.9798850574712644, "score": 676.0, "evalue": 4.5e-248, "description": "Internal stop codon at 638452, 638563, 638701. Nonsense mutation around 638452, 638563, 638701.", "psc": { "uniref90_id": "UniRef90_D1J8U7", "gene": "ArgE", "product": "Xaa-His dipeptidase", "uniref50_id": "UniRef50_A0A448ZY24", "cog_id": "COG0624", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8U7", "UniRef:UniRef50_A0A448ZY24", "COG:COG0624", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZY24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZY24" ], "product": "Dipeptidase SA1572" } }, "id": "AHCKFKLKGB_1098", "locus": "GCA013367975_01097" }, { "type": "cds", "sequence": "contig_1", "start": 638699, "stop": 638998, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTTATTACTTATAAGCAAAGTAAAGAAGACTTTGATGAAATGATTAAAAATATTGCAAATATTTGTGCAATTCCTTCAATTAGTGAAGAAGATTTTAGCAGTGAATTTCCTTTTGGAAAAGAAACTGACAATGCCTTAAATTATGCATTAAATCTTGCCAAAAGTTTTGGATTTAATATTTATAAAGATCCTAAAAATCGTTATGGGTTTGCTGAATTTGGCAATGGAGATAAGATTATTGGAATACTAGCCCACCTTGACGTTGTTCCTGCAGGAGATGAAAGCCAATGA", "aa": "MKKFITYKQSKEDFDEMIKNIANICAIPSISEEDFSSEFPFGKETDNALNYALNLAKSFGFNIYKDPKNRYGFAEFGNGDKIIGILAHLDVVPAGDESQ", "aa_hexdigest": "6d5702e55358a66b66de891d7d2c573f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11122.363599999988, "isoelectric_point": 4.749943733215332 }, "id": "AHCKFKLKGB_1099", "locus": "GCA013367975_01098" }, { "type": "cds", "sequence": "contig_1", "start": 639249, "stop": 639488, "strand": "-", "frame": 2, "gene": null, "product": "endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00068CCCF8", "UniRef:UniRef90_UPI00068CCCF8" ], "nt": "TTGCAAGGCAACAAAATATCCAAAAACGGTACAAAAACTGGTTTGTCAGTTGAAGATGGTGGACCTGTTACTGAACCTATTAATGAATTTAAGGGTGATGTTGCTAGGGCATATTTATACTTTTGTTTAACTTATAACGATTTAAATATAACATCAAGTGATCAATCAACTCGTGTATTTAAAGAAAATAATGGAAAAAATACTATAACTCCTATATTTTTAAAAACATTTTTAGCTTGA", "aa": "MQGNKISKNGTKTGLSVEDGGPVTEPINEFKGDVARAYLYFCLTYNDLNITSSDQSTRVFKENNGKNTITPIFLKTFLA", "aa_hexdigest": "efe6bab7154c4cd929b2bfbb03618e1b", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 639075, "stop": 639556, "observations": { "deletion": [ 639541 ], "stop": [ 639250 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00068CCCF8", "query_cov": 1.0, "subject_cov": 0.49375, "identity": 0.937, "score": 149.0, "evalue": 1.42e-44, "gene_start": 1, "gene_end": 79, "reference_start": 24, "reference_end": 102, "reference_sequence": "MFDQPDKLVNAWHANYPYGTVKELQGNKISKNGTKTGLSVEDGGPVTEPINEFKGDVARAYLYFCLTYNDLNITSNNQSTRVFKEINRKNTITPIFLKTFLAWNNLDKVSKFDIDRNNGVYKHQGNRNPFIDYPKLIDVVYNNNNDYVFKNKGIAVGLNK" }, "paralog": false, "identity": 0.9625, "score": 315.0, "evalue": 2.33e-112, "description": "Internal stop codon at 639250. Frameshift due to deletion around 639541.", "psc": { "uniref90_id": "UniRef90_UPI00068CCCF8", "product": "endonuclease", "uniref50_id": "UniRef50_UPI00068CCCF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00068CCCF8", "UniRef:UniRef50_UPI00068CCCF8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00068CCCF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00068CCCF8" ], "product": "endonuclease" } }, "id": "AHCKFKLKGB_1100", "locus": "GCA013367975_01099" }, { "type": "cds", "sequence": "contig_1", "start": 639669, "stop": 640400, "strand": "-", "frame": 2, "gene": null, "product": "endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAK4", "UniRef:UniRef90_UPI002410E520" ], "nt": "ATGAAAAAAACCAAAAAAATATTCCTAACTATGGGTGCTATTTTACCTTTGCTAATTTCAGCGCCATTAGTTGCTGCTGGATGTGTAAAAACAAAGAAATTTGAAGTTAAACCAAAAGGTGAAACCACAATAGAAAACATCATTAATAAACATAAAGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTATTAGATCAATTTAAAAAATCAATTGAAGAAAGAACAAACCAAGCAGCATTAAACGAAGCAAAGAAGCAATTAAATTCAACTTTAAAACATGCGGCAAAAATAAATACAAAATCATTTAATTTTAACCAAAAATTTAATTTTAATAAAGAATATGTGAAAGGATTAGAAGCAATTAAAGGTGATGATTTGAATGCTATTAAAGAAATTACATTCAGATTAAATAGTTTTATAAATTTGGCGACATCACAAAAACACGGAACAACCCCTGAAAAGAAGCCCGAAGCTAAACCCTTAGATAATAAGATAATTTACGATAATTCAAATGACTTTTATAAATCCTTAAATGGCTTAAGTGGACAAAACCTAAGAGATAACTTATTTGCATTGCAAAAAGCTCATAGAAATCACTCAACAAAATATAAAGACTTATTTGATACATATAAAGATGCTTTTGTTGATAAATATTACGAAAAAGATGGATCGGTTTTAGATATCTATGAAGAAATTCCGGATAAAGAAGATGTTTTTACTAATTGA", "aa": "MKKTKKIFLTMGAILPLLISAPLVAAGCVKTKKFEVKPKGETTIENIINKHKEIKDDELNKTKSLLDQFKKSIEERTNQAALNEAKKQLNSTLKHAAKINTKSFNFNQKFNFNKEYVKGLEAIKGDDLNAIKEITFRLNSFINLATSQKHGTTPEKKPEAKPLDNKIIYDNSNDFYKSLNGLSGQNLRDNLFALQKAHRNHSTKYKDLFDTYKDAFVDKYYEKDGSVLDIYEEIPDKEDVFTN", "aa_hexdigest": "1cf8438a87cd78cae69db2a47b5b8c6c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 639366, "stop": 640400, "observations": { "mutation": [ 639551, 639596, 639671 ], "stop": [ 639551, 639596, 639671 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410E520", "query_cov": 1.0, "subject_cov": 0.5510204081632653, "identity": 1.0, "score": 446.0, "evalue": 1.05e-154, "gene_start": 1, "gene_end": 243, "reference_start": 1, "reference_end": 243, "reference_sequence": "MKKTKKIFLTMGAILPLLISAPLVAAGCVKTKKFEVKPKGETTIENIINKHKEIKDDELNKTKSLLDQFKKSIEERTNQAALNEAKKQLNSTLKHAAKINTKSFNFNQKFNFNKEYVKGLEAIKGDDLNAIKEITFRLNSFINLATSQKHGTTPEKKPEAKPLDNKIIYDNSNDFYKSLNGLSGQNLRDNLFALQKAHRNHSTKYKDLFDTYKDAFVDKYYEKDGSVLDIYEEIPDKEDVFTNWHGKYRDQGKYEGDGMNREHLVPQSWFSKSVPMRTDAHHVWPTDKLVNAWHANYPYGTVKELQGNKISKNGTKTGLSVEDGGPVTEPINEFKGDVARAYLYFCLTYNDLNITSSDQSTRVFKENNGKNTITPIFLKTFLAWNNLDKVSKFDIDRNNGVYKHQGNRNPFIDYPELIDVVYNNNNDYVFKNKGIAVGLNK" }, "paralog": false, "identity": 0.9912790697674418, "score": 667.0, "evalue": 1.86e-244, "description": "Internal stop codon at 639551, 639596, 639671. Nonsense mutation around 639551, 639596, 639671.", "psc": { "uniref90_id": "UniRef90_UPI002410E520", "product": "endonuclease", "uniref50_id": "UniRef50_A0A454CAK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410E520", "UniRef:UniRef50_A0A454CAK4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454CAK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAK4" ], "product": "Ribonuclease" } }, "id": "AHCKFKLKGB_1101", "locus": "GCA013367975_01100" }, { "type": "cds", "sequence": "contig_1", "start": 640558, "stop": 640671, "strand": "-", "frame": 1, "gene": "marR", "product": "MarR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B85C5", "UniRef:UniRef100_O85035", "UniRef:UniRef50_O85035", "UniRef:UniRef90_O85035" ], "nt": "ATGAGAAAAGACGTTAGATTAGCTAACAAGGTAAAAGCAGCTAAAAGAGAACATAAATTAGCTTTAGAAAAAGCCAGAGCTCGCCGTAGACTTGCTAAACAACAAGCTGAATAA", "aa": "MRKDVRLANKVKAAKREHKLALEKARARRRLAKQQAE", "aa_hexdigest": "96ab6046e0d54d5a6a1bff54ed7fd2fb", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000B85C5", "uniref100_id": "UniRef100_O85035", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B85C5", "UniRef:UniRef100_O85035" ] }, "ips": { "uniref100_id": "UniRef100_O85035", "uniref90_id": "UniRef90_O85035" }, "psc": { "uniref90_id": "UniRef90_O85035", "product": "MarR family transcriptional regulator", "uniref50_id": "UniRef50_O85035" }, "pscc": { "uniref50_id": "UniRef50_O85035", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O85035" ], "product": "MarR family transcriptional regulator" }, "genes": [ "marR" ], "id": "AHCKFKLKGB_1102", "locus": "GCA013367975_01101" }, { "type": "cds", "sequence": "contig_1", "start": 640708, "stop": 640965, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTGCTGAACATGCTTTGATTAAAAAATCCTATAATCAAGATATTCAACATTTAAATAACGAAGAACAAGAAGAACTATTTCAAATATCAACAGAAGATATTGAATTACCAAATGTTTCAAATAATGTTAATGACAACCAAAAAAATAAAAAAAATATAGCAACAAAAGAAAGCATTAGTCAGAAAATACAAGAAATTGAAAAAATAGATCTTGAAACAATAATGCAAAAAATTAAACAAATTAAGAAAAAATAG", "aa": "MTAEHALIKKSYNQDIQHLNNEEQEELFQISTEDIELPNVSNNVNDNQKNKKNIATKESISQKIQEIEKIDLETIMQKIKQIKKK", "aa_hexdigest": "d3497e7ba17bf8d0558e3097ddb389a8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9978.157399999995, "isoelectric_point": 5.678749656677247 }, "id": "AHCKFKLKGB_1103", "locus": "GCA013367975_01102" }, { "type": "cds", "sequence": "contig_1", "start": 641143, "stop": 641565, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGTTTAAAGATGATAATGATGAATTAATAGATGCTCAAATTTCTAATGATGAAAAAATGCTATTTATTTTACTAAATAATGGTATGTATCATTTAGTTTCAGAAAACGAACTAAAGGTTGGGATTTCTTTGAAAGCAAGAGGCATTAGACTTCTTTTAAACTTATATAAACATCCTCAACTTCAAGTAAGTGGTTTTATAACAGTTTCACAATACAACAATATAATTTATTTAACGCAAGGCGGCTATATAAAATGTTGGGATACTAGCAAATTAGAATTGACCACACGTAATACACCAAAAATGTTGTTTACGCCACTAAAGAATAATATTTTAGGTCTTCAATCACTTGCTGTTACATTGAGCAATTTAAAAATGTTATACACTGATAATAATGGTAATTTGGCAGAATATGATTGA", "aa": "MSFKDDNDELIDAQISNDEKMLFILLNNGMYHLVSENELKVGISLKARGIRLLLNLYKHPQLQVSGFITVSQYNNIIYLTQGGYIKCWDTSKLELTTRNTPKMLFTPLKNNILGLQSLAVTLSNLKMLYTDNNGNLAEYD", "aa_hexdigest": "60355d2c95a7000871c6694408e8c29b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15963.223599999983, "isoelectric_point": 6.087819862365721 }, "id": "AHCKFKLKGB_1104", "locus": "GCA013367975_01103" }, { "type": "cds", "sequence": "contig_1", "start": 641752, "stop": 642063, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGAAATAAAAAATCAATATTCAAAGCCAAGATTAACGGAAATTTCAGATCAAAAAATTGATAAAGAAATTGATCATAAATTATTGACAAAAAATGAAGATTTTTATTTATATATAACCAAAGATGGATATTATAAAAAAATAAGTTTAAAAGTTTATACTAGCAATGAATTAAGCACATTCAAATTAAAAGAAGAAGATAATGTTTTCTATTTTGATAAAGTAAACTCATTATCAAAGATATTATTCTTTACAAATTTAGGAAATTATTTTATTATTGATTGCCATTTATTTAAAGATTGCAATTGA", "aa": "MLEIKNQYSKPRLTEISDQKIDKEIDHKLLTKNEDFYLYITKDGYYKKISLKVYTSNELSTFKLKEEDNVFYFDKVNSLSKILFFTNLGNYFIIDCHLFKDCN", "aa_hexdigest": "4ad2c66b55dc1167e617b233a33f0d58", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12401.070599999985, "isoelectric_point": 6.718846321105957 }, "id": "AHCKFKLKGB_1105", "locus": "GCA013367975_01104" }, { "type": "cds", "sequence": "contig_1", "start": 642091, "stop": 643173, "strand": "-", "frame": 1, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B04E97" ], "nt": "ATGCATGGTAATAAAGGTTCAATTGATGATGACCCTGCCGCTGCAATGCGTTACACTGAATCAAGACTTGAAAAAATTAGTGAATTGATGTTGAGAGATTTAGACAGAAAAGTTGTAAAAATGGCTCCAAACTTTGATGACTCTGAATACGAACCAATTGTTTTGCCGGCCTTATTTCCTAATTTATTAGTTAACGGTGCTAAGGGAATTGCTGCTGGCTTTGCTACAGAAATCCCACCACATAATCTAGGTGAAGTTATTGATGCAACAATTGCATTAATCAAAAATCCTACAATATCAATTGAAGAATTAAGTGAAATAGTTAAAGGCCCAGATTTCCCAACAGGAGCAATTATTAATGGTATAAATGAAATAAAAAAAGCTCTTTCAAGTGGGCAAGGTAGAATTACAATTTCTTCGAAATATCATTATGTTTATGATAAAAAAGATGAATCGAAAATTATTGGTATTGAAATAATCGAAATTCCTTTTGGCGTAGTTAAATCAAAATTAGTTGCCGACATTGATGCGATTGCAATAGATAAAAAAATTTCTGGTATTAAAGAAGTTTTAGACCAAACAGATAGAAACGGAATTTCAATATTTATTCAATTAGAAGACGGTGCAAATGCTGACGCAATAATTGCATACTTAATGAATAAAACCGAACTAAGTATCTCATATAGTTATAACATGGTTGCAATTGACAATAACCGCCCAGTAATTTTAAATCTCTATAGTGCTTTAATTGCTTATTTAAGTCATTTAAAAGAAGTTAATATAAATGGTATTAATTATGATTTAAAGAAGTTTAAATTAAGACTAGAAATAGTTGAAGGATTCATTAAAGTAGCTGAAATTTCTGATGAAGTTATACATTTGATTAAAGAAAGTGATAACTCAAAAAAAGGTGTTATCCTTGCATTGATGAATAGATTTAAATTTAGTGAATTGCAAGCAACAGCGATTGCTGAATTAAGATTGTATAAGCTTTCAAGAATGGATCAAATTGAATTTCAAGAAGAAAAGAAAAACCTTGAAATTCAAATTGAAAATTGCAATAAATTATTAAATGATAAATGA", "aa": "MHGNKGSIDDDPAAAMRYTESRLEKISELMLRDLDRKVVKMAPNFDDSEYEPIVLPALFPNLLVNGAKGIAAGFATEIPPHNLGEVIDATIALIKNPTISIEELSEIVKGPDFPTGAIINGINEIKKALSSGQGRITISSKYHYVYDKKDESKIIGIEIIEIPFGVVKSKLVADIDAIAIDKKISGIKEVLDQTDRNGISIFIQLEDGANADAIIAYLMNKTELSISYSYNMVAIDNNRPVILNLYSALIAYLSHLKEVNINGINYDLKKFKLRLEIVEGFIKVAEISDEVIHLIKESDNSKKGVILALMNRFKFSELQATAIAELRLYKLSRMDQIEFQEEKKNLEIQIENCNKLLNDK", "aa_hexdigest": "c55bf18edc6f3a4bfb3c63048aad9a13", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021B04E97", "query_cov": 0.8611111111111112, "subject_cov": 1.0064935064935066, "identity": 0.506, "score": 299.0, "evalue": 4.35e-97, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_UPI0021B04E97" }, "pscc": { "uniref50_id": "UniRef50_UPI0021B04E97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B04E97" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "AHCKFKLKGB_1106", "locus": "GCA013367975_01105" }, { "type": "cds", "sequence": "contig_1", "start": 643327, "stop": 643509, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "ATGAAAAAAGATAGAAAAGAAGAAATACAAGAAGTTACTGAAAACATTATTGAAAAAAATATGGCCGATATAATGTCTGATAGATTCGGACGTTATTCAAAATACATTATTCAACAAAGAGCAATTCCTGATGCTCGTGATGGACTAAAACCTGTTCAACGTCGGATTTTATATTCAATGTGA", "aa": "MKKDRKEEIQEVTENIIEKNMADIMSDRFGRYSKYIIQQRAIPDARDGLKPVQRRILYSM", "aa_hexdigest": "8d3587316b5efea3fb752e3d36364935", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 21, "aa_cov": 0.35, "hmm_cov": 1.0, "evalue": 9.592815379633228e-11, "score": 36.42316436767578, "start": 40, "stop": 60 } ], "seq_stats": { "molecular_weight": 7217.295900000001, "isoelectric_point": 9.451403617858887 }, "id": "AHCKFKLKGB_1107", "locus": "GCA013367975_01106" }, { "type": "cds", "sequence": "contig_1", "start": 643572, "stop": 643682, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCCCGAAACTCGTTCAATAATTAAAGTTGATGTAGAAGATGAAATTTTAGTTGAAAGAAATGTTTCAACATTGATGAGTGACGACGTAATAGCAAGAAAACAATGA", "aa": "MNPETRSIIKVDVEDEILVERNVSTLMSDDVIARKQ", "aa_hexdigest": "099d210941b061bc58644c32aaae5669", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4144.682400000001, "isoelectric_point": 4.507070732116699 }, "id": "AHCKFKLKGB_1108", "locus": "GCA013367975_01107" }, { "type": "cds", "sequence": "contig_1", "start": 643692, "stop": 643886, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTAGTTGAAAATGGTATGGTCTATATTGCCTTGCCACCTCTTTATAAGATTTCAATAAAATCAAAAAATACACAACCCCTTTATGCTTGGGATGAAGATCAATTAAAAGAAATTACTTCAAAATATACCAACTATGAAATTCAACGCTACAAAGGGCTTGGTGAGATGAATGCTGATCAACTTTGA", "aa": "MKKLVENGMVYIALPPLYKISIKSKNTQPLYAWDEDQLKEITSKYTNYEIQRYKGLGEMNADQL", "aa_hexdigest": "62171427d03e6284d882c45f788b6bc4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7514.605900000001, "isoelectric_point": 7.907514762878418 }, "id": "AHCKFKLKGB_1109", "locus": "GCA013367975_01108" }, { "type": "cds", "sequence": "contig_1", "start": 643893, "stop": 644849, "strand": "-", "frame": 2, "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "COG:COG0187", "COG:L", "SO:0001217", "UniRef:UniRef50_Q59961", "UniRef:UniRef90_D1J8V0" ], "nt": "ATGCCAAATCTTACTATTGAATTTTATAATGAAAATACCGATGAACATGTAACATTTAAAACCAAAGATGGCATAAAAGAATATATAAATTTTGTTGGTGAAGGCAAAAAATTTATTAGTGAAATTTTTTATGCATCTGGTATGTGCAATTACGGCATTGAAATTGACTTGGCATTAGTATATACTGATGGATATAGTGAATCAATTTACAGTTTTGTAAATAATGTAAAGACACGTGATGGTGGAACACATGAAACAGCACTTAAAACCGCATTAACTAAGACAATAAATGATTATGCTTCTAAAAATGGTTTAGCTAAAAAAATAACTTTTGAAGGATCGGATGTTAGAGAAGGAATAATTGCTGTTATTTCTTTAAAAGTCCCAGAAGAAATATTGGAATTTGTAGGTCAAACGAAAGATAAATTAGGTACGCCAGAGGCTAGAGAAGTTGTTGAAGATTTTGTAAGTCAAAAGTTTTATTTCTTTTTAAATGAAAACAAAATTGAAGCAGAAAAAATTATTTCTAAAATTAAAAAAGCATACGAAGCAAAAGTTGCAGCTAGAAACGCAAGAAACGAAGCACGTAAGATTAAAAATAAATTTGAGAATAGAAAGATTCTTTCAGGAAAATTAACTCCTGCTCAAAGTAAAAATCCTGCCATTAAAGAATTGTTTTTAGTTGAAGGTGATTCAGCTGGTGGTTCTGCTAAATTAGGAAGAAATAGAGTTACTCAGGCAATATTGCCACTTAGAGGTAAAGTTATCAACACCGATAAAGCAAAATTAAGCGATGTTTTAGCAAATGAAGAAATTGCAACAATAATTAATACAATCGGAGCAGGTATAGGTGAAGACTTCAATTTAAAAAATGCTCAATATCACAAAATTATTATTATGACAGATGCTGATACTGATGGTGCTCACATTCAAACATTACTATTAACATTCTTTTGA", "aa": "MPNLTIEFYNENTDEHVTFKTKDGIKEYINFVGEGKKFISEIFYASGMCNYGIEIDLALVYTDGYSESIYSFVNNVKTRDGGTHETALKTALTKTINDYASKNGLAKKITFEGSDVREGIIAVISLKVPEEILEFVGQTKDKLGTPEAREVVEDFVSQKFYFFLNENKIEAEKIISKIKKAYEAKVAARNARNEARKIKNKFENRKILSGKLTPAQSKNPAIKELFLVEGDSAGGSAKLGRNRVTQAILPLRGKVINTDKAKLSDVLANEEIATIINTIGAGIGEDFNLKNAQYHKIIIMTDADTDGAHIQTLLLTFF", "aa_hexdigest": "6e326102553164995e849329219f0ca6", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI001E534FD0", "query_cov": 0.9842767295597484, "subject_cov": 0.8368983957219251, "identity": 0.534, "score": 318.0, "evalue": 4.62e-104, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_A0A380FFC7" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 643590, "stop": 645149, "observations": { "mutation": [ 643694, 643895, 644930, 645035 ], "stop": [ 643694, 643895, 644930, 645035 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8V0", "query_cov": 1.0, "subject_cov": 0.49765258215962443, "identity": 1.0, "score": 610.0, "evalue": 3.59e-215, "gene_start": 1, "gene_end": 318, "reference_start": 196, "reference_end": 513, "reference_sequence": "MSLNNYEASDLKVLKGLDAVRKRPGMYIGSTDSSGLHHLIWEIFDNAIDEALSGFANKIIVTLKKDSSVIVEDNGRGIPVNKHESGKSGVELVFTELHAGGKFGDGAYKTSGGLHGVGSSVVNALSSKVEVTVWRDKKAYFTAFQQDTIIEKTHEISPTTKQGTKVQFWADFSIFKKSKYSPEIIKEKLREASFLMPNLTIEFYNENTDEHVTFKTKDGIKEYINFVGEGKKFISEIFYASGMCNYGIEIDLALVYTDGYSESIYSFVNNVKTRDGGTHETALKTALTKTINDYASKNGLAKKITFEGSDVREGIIAVISLKVPEEILEFVGQTKDKLGTPEAREVVEDFVSQKFYFFLNENKIEAEKIISKIKKAYEAKVAARNARNEARKIKNKFENRKILSGKLTPAQSKNPAIKELFLVEGDSAGGSAKLGRNRVTQAILPLRGKVINTDKAKLSDVLANEEIATIINTIGAGIGEDFNLKNAQYHKIIIMTDADTDGAHIQTLLLTFFWRYMKKLVENGMVYIALPPLYKISIKSKNTQPLYAWDEDQLKEITSKYTNYEIQRYKGLGEMNADQLWETTMNPETRSIIKVDVEDEILVERNVSTLMSDDVIARKQWINNNVDFSSEDDFKINNK" }, "paralog": false, "identity": 0.9922928709055877, "score": 1002.0, "evalue": 0.0, "description": "Internal stop codon at 643694, 643895, 644930, 645035. Nonsense mutation around 643694, 643895, 644930, 645035.", "psc": { "uniref90_id": "UniRef90_D1J8V0", "gene": "GyrB", "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_Q59961", "cog_id": "COG0187", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8V0", "UniRef:UniRef50_Q59961", "COG:COG0187", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_Q59961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q59961" ], "product": "DNA topoisomerase 4 subunit B" } }, "id": "AHCKFKLKGB_1110", "locus": "GCA013367975_01109" }, { "type": "cds", "sequence": "contig_1", "start": 645033, "stop": 645434, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGAGTTTAAATAATTACGAAGCTAGCGATTTAAAAGTTTTAAAAGGATTAGATGCTGTCAGAAAAAGACCGGGAATGTATATTGGGTCTACTGATTCAAGTGGGCTTCATCACTTAATTTGGGAAATTTTTGATAATGCTATTGATGAAGCGTTGAGTGGGTTTGCAAATAAAATAATTGTAACTTTAAAGAAAGATTCTTCTGTTATTGTTGAAGACAACGGTAGGGGAATTCCTGTCAATAAACACGAGTCTGGAAAAAGTGGAGTTGAGCTTGTCTTTACTGAACTTCATGCTGGTGGTAAATTTGGCGATGGTGCTTATAAAACAAGCGGGGGATTACACGGAGTAGGTTCCTCGGTTGTCAATGCCTTAAGTTCTAAAGTTGAAGTTACGGTTTGA", "aa": "MSLNNYEASDLKVLKGLDAVRKRPGMYIGSTDSSGLHHLIWEIFDNAIDEALSGFANKIIVTLKKDSSVIVEDNGRGIPVNKHESGKSGVELVFTELHAGGKFGDGAYKTSGGLHGVGSSVVNALSSKVEVTV", "aa_hexdigest": "5ba540597d72b901175e8134d81c6abe", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00037E1229", "query_cov": 1.0, "subject_cov": 0.8012048192771084, "identity": 0.677, "score": 187.0, "evalue": 1.9e-58, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A962E3V0" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 101, "aa_cov": 0.7368421052631579, "hmm_cov": 0.8514851485148515, "evalue": 1.3271380854621718e-17, "score": 59.5620231628418, "start": 32, "stop": 129 } ], "seq_stats": { "molecular_weight": 14056.700999999986, "isoelectric_point": 6.182343101501464 }, "id": "AHCKFKLKGB_1111", "locus": "GCA013367975_01110" }, { "type": "cds", "sequence": "contig_1", "start": 645488, "stop": 645946, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTGTTCTTTAAGTGATTTATTAGTAGTTAAATACTACTTCAACGAACCGTATGATTCAATATTTAAATCTAATAATTTTATAAGAGATTTAAGATTAATAGGTAAGGCTAAGGATTATGTAATTGACTTTGCTTTTTGCGACAGAAATGTTATAAATTCAAATAAAAAAAATATATATAAATTAATTAAAAAAGAATTCAACAACGGCAAAGATTTAACATTGATTGTAGGCCACGATACTAACATTGCTACGACCTTAGCAATGTTAGACGTCAAAATGCCAGATCTTAAACAATTAGAAAAATATCCAATTGGGTCTAAATTAATTTTTGCAATTAATGATGACAATAGTTTTGATTTGTATATTGCTTTTTTTGATTTTAAAGACATTAGAAACTTTAATCTTTCAAATCCACAAATTATAAAAATCGCAAGCAAAATTTTTCTAAAATAA", "aa": "MACSLSDLLVVKYYFNEPYDSIFKSNNFIRDLRLIGKAKDYVIDFAFCDRNVINSNKKNIYKLIKKEFNNGKDLTLIVGHDTNIATTLAMLDVKMPDLKQLEKYPIGSKLIFAINDDNSFDLYIAFFDFKDIRNFNLSNPQIIKIASKIFLK", "aa_hexdigest": "c74c8fd435a5f6d4be766bc475c6c257", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17639.33969999999, "isoelectric_point": 8.953707695007324 }, "id": "AHCKFKLKGB_1112", "locus": "GCA013367975_01111" }, { "type": "cds", "sequence": "contig_1", "start": 646139, "stop": 646315, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCAAAAAATTAGAGAATTTTTCAATATAACAGATGATATTGATGTTAAGGTGATGGCAAATTCAACGCAAAGAACTTTTCAAACTGCGCAGTTACTTTCAATTGGCCTTTTTCCTGCTAAAAAGGTTGACATTCAATGTCAAGATTATTCATTCAAAAATGAGAATGAATGA", "aa": "MAQKIREFFNITDDIDVKVMANSTQRTFQTAQLLSIGLFPAKKVDIQCQDYSFKNENE", "aa_hexdigest": "bd472e7ab471b738f75377dd4b19035e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6730.569299999999, "isoelectric_point": 5.214375114440917 }, "id": "AHCKFKLKGB_1113", "locus": "GCA013367975_01112" }, { "type": "cds", "sequence": "contig_1", "start": 646590, "stop": 646847, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00044.30" ], "nt": "ATGAAAAAAACTAGAATTGCAATTAACGGTTTTGGTAGAATTGGTCGTCTAATTTTTAGAGAAATTTTTAATGACCCTGAATTTGAAATCGTTGCTATTAATGACTTAACAGATGCAGCTACATTGGCTCACTTATTGAAATACGATACAGCTCATGGAAAATTGAATGAAAATATTTCACACACAGAAAATTCTATTGTTGTAAATGGCAAAGAATATCGTGTTTATAGTGAAAAAGATCCATTAAACCTACCTTGA", "aa": "MKKTRIAINGFGRIGRLIFREIFNDPEFEIVAINDLTDAATLAHLLKYDTAHGKLNENISHTENSIVVNGKEYRVYSEKDPLNLP", "aa_hexdigest": "3dbf16e2745d0c9426324094fa009409", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BE2B32", "query_cov": 0.9529411764705882, "subject_cov": 0.826530612244898, "identity": 0.679, "score": 115.0, "evalue": 7.04e-32, "valid": false, "product": "glyceraldehyde 3-phosphate dehydrogenase NAD-binding domain-containing protein", "uniref50_id": "UniRef50_A0A2M8L0B5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00044.30", "name": "Gp_dh_N", "length": 81, "aa_cov": 0.9529411764705882, "hmm_cov": 1.0, "evalue": 1.1860310838137719e-32, "score": 107.5826187133789, "start": 5, "stop": 85 } ], "seq_stats": { "molecular_weight": 9670.886199999995, "isoelectric_point": 6.267487907409667 }, "id": "AHCKFKLKGB_1114", "locus": "GCA013367975_01113" }, { "type": "cds", "sequence": "contig_1", "start": 646914, "stop": 647525, "strand": "+", "frame": 3, "gene": null, "product": "NAD-dependent glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380FCW9" ], "nt": "TTGCACATTCAAGCAGGTGCTAAAAAGGTATTTATTACTGCTCCAGCTAAAAGCGAAGGTGTTAAAACAGTTGTTTATTCAGTAAACGAAGATATCATTACGCCAGAAGATAAAATTTTATCAGGTGCTTCATGTACTACTAACTGTTTAGCTCCTATTGCCAACGTATTGGAAAAAAACTTTGGTATTGAAAAAGGATTTATGACAACAGTTCACTCATATACAGCAGACCAAAGATTACAAGATGCTCCACACAAAGATTTAAGAAGAGCAAGAGCAGCTGCTTCAAACATGGTTCCAACAACAACTGGTGCTGCAATAGCAATTGGAAAGGTTATTCCTTCATTGGATAAAAAACTAAATGGACTATCTCTAAGAGTTCCAACAATAACTGGATCATTCGTTGATTTAACTGTTGAATTAAAGAAAGATGCTACTGTTCAAGAAATTAATGAAGCAATGAAGAAATCTGCTTCTGAATCATTAGAATATTCTACAGAACCAATTGTATCAAGTGATATTATTGGTGCAAAAGCAGGCTCAATATTTGACTCACTTTTAACAAGTGTGTTAGAAGTAGAAGGCAAAAAACTATACAAAGTTTATGCATGA", "aa": "MHIQAGAKKVFITAPAKSEGVKTVVYSVNEDIITPEDKILSGASCTTNCLAPIANVLEKNFGIEKGFMTTVHSYTADQRLQDAPHKDLRRARAAASNMVPTTTGAAIAIGKVIPSLDKKLNGLSLRVPTITGSFVDLTVELKKDATVQEINEAMKKSASESLEYSTEPIVSSDIIGAKAGSIFDSLLTSVLEVEGKKLYKVYA", "aa_hexdigest": "782578356ec56521c60ae06f970bcc35", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_A0A380FCW9", "query_cov": 1.0049261083743843, "subject_cov": 0.816, "identity": 0.529, "score": 208.0, "evalue": 1.78e-64, "valid": false, "product": "NAD-dependent glyceraldehyde-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A380FCW9" }, "pscc": { "uniref50_id": "UniRef50_A0A380FCW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380FCW9" ], "product": "NAD-dependent glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [], "id": "AHCKFKLKGB_1115", "locus": "GCA013367975_01114" }, { "type": "cds", "sequence": "contig_1", "start": 647620, "stop": 648108, "strand": "+", "frame": 1, "gene": null, "product": "Septicolysin", "db_xrefs": [ "RefSeq:WP_044329897.1", "SO:0001217", "UniParc:UPI0005C7F276", "UniRef:UniRef100_UPI0005C7F276", "UniRef:UniRef50_D1J8V3", "UniRef:UniRef90_D1J8V3" ], "nt": "ATGAAACTTTCAATAAAAGAAATAATTAAAAAAATTAAATTACTTGAATTGGATCTTGAAGATCTAAAAAAAGAAGAATCCAAGGAAAACTTCATTGTTTATTTAGAAAACAAAAGACCAACGAACATTGAATATGATTATCAAGAATATTCAAACAAAATTAAAAATTTATATAATGAGATTTTTAAGTTAAGAACATTGGTTCAAGTAACTAATATAAACACAATAACATCATATAAAAAACATTCTATATCAGAATTAATTATTTTATTAGCTCAAAAAAGTAATCAATTAGAAAGATTGCAAGAAATGCGCGATTGCAAAAAAATTTCTAGGGTTACTACAAACGATGGCCAAAACGAATATACTGAATATCTTTTTGACCCTAAAATGGTTGCAATTGAACTTAGAAAATTAAACAAAGAAATTTCTGAAATTCAAATTGCAATTGATAGCGCAAACATTAATACATTAGTTGAAGTTAAGTAA", "aa": "MKLSIKEIIKKIKLLELDLEDLKKEESKENFIVYLENKRPTNIEYDYQEYSNKIKNLYNEIFKLRTLVQVTNINTITSYKKHSISELIILLAQKSNQLERLQEMRDCKKISRVTTNDGQNEYTEYLFDPKMVAIELRKLNKEISEIQIAIDSANINTLVEVK", "aa_hexdigest": "ac92853774d857381411fa2f38e58996", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0005C7F276", "ncbi_nrp_id": "WP_044329897.1", "uniref100_id": "UniRef100_UPI0005C7F276", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C7F276", "RefSeq:WP_044329897.1", "UniRef:UniRef100_UPI0005C7F276" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C7F276", "uniref90_id": "UniRef90_D1J8V3" }, "psc": { "uniref90_id": "UniRef90_D1J8V3", "product": "Septicolysin", "uniref50_id": "UniRef50_D1J8V3" }, "pscc": { "uniref50_id": "UniRef50_D1J8V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8V3" ], "product": "Septicolysin" }, "genes": [], "id": "AHCKFKLKGB_1116", "locus": "GCA013367975_01115" }, { "type": "cds", "sequence": "contig_1", "start": 648355, "stop": 648537, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACAACAAAAGTTTACATAGATTTTGAAGCAATTACCAATCCTTTTGCAAGATTAGTAAATTTGCCAAGTGGAACTCCTTATGCCTATACATTAGGTCTTTTAAATATTCACGGCCAATATCAAATTAAAACATTTATTATCGATTTTAAAGAACATCATAATTTAAATTCAATTTGA", "aa": "MNTTKVYIDFEAITNPFARLVNLPSGTPYAYTLGLLNIHGQYQIKTFIIDFKEHHNLNSI", "aa_hexdigest": "8990b4dae1576a3af97b68c45f302653", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6897.818599999997, "isoelectric_point": 6.8117780685424805 }, "id": "AHCKFKLKGB_1117", "locus": "GCA013367975_01116" }, { "type": "cds", "sequence": "contig_1", "start": 648589, "stop": 648846, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATTTAAAGGAAGTTGTATTTGTAGGTCATAATCCTGTTTTAGAAAATAAATGCTTAAAAAAATTATTTCCTGAAAATAATGTTGAACCTTTAATTTCCAATACAATTGTGTCATTGTCAAAATTAACAGGGAAAATTTTTAACATTCCATATTTTAATAAAATAAAAAAAATTATCGCTGATTCTGGCGATAGCGAACTTAATATGAGAATTAAAGATAGAAATGGTGTAATTGCTGCATTTGCTGGATATTGA", "aa": "MNLKEVVFVGHNPVLENKCLKKLFPENNVEPLISNTIVSLSKLTGKIFNIPYFNKIKKIIADSGDSELNMRIKDRNGVIAAFAGY", "aa_hexdigest": "eb894e435dfdfc2f3667dc164b07c035", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9522.076899999993, "isoelectric_point": 9.37423496246338 }, "id": "AHCKFKLKGB_1118", "locus": "GCA013367975_01117" }, { "type": "cds", "sequence": "contig_1", "start": 648910, "stop": 649110, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAATCTTTTGTTAAAAGAATTAAAACGTTATTCTCATGACGATGTCAACAAAATGCTTTATATTGTTTCTCATCCTGATGAAACAGAAACTTTACTAAAAGAACTTTTATACAAACGTGAACTAATAAAACAAATAAAAAATTTGGAACTTGATGACAATTTAACAATTAAAGAAATTAAAGAAAAAATTTGA", "aa": "MNKNLLLKELKRYSHDDVNKMLYIVSHPDETETLLKELLYKRELIKQIKNLELDDNLTIKEIKEKI", "aa_hexdigest": "c5f9e23ce5844c46f86b1d1e4514c0d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7996.301299999995, "isoelectric_point": 6.728338432312013 }, "id": "AHCKFKLKGB_1119", "locus": "GCA013367975_01118" }, { "type": "cds", "sequence": "contig_1", "start": 649237, "stop": 649467, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATAATAATTGGCGAAATTATATTTAATTTAAATGGGCTACTTGATAATTTCATGGTTAGCCATATTGATGGTGGAATTGATGCCTTGGCTTATGCTAATACATATACGGGAATAATTTATACAATTTTCTTTTCTATTCAAGCAATCGGGGCAATGTTCGTGGGTCAATATTATGGCCGTAAAGACTTTGATAAAGTAAAGCAGATAATGAATTTAAGAATTTGA", "aa": "MPIIIGEIIFNLNGLLDNFMVSHIDGGIDALAYANTYTGIIYTIFFSIQAIGAMFVGQYYGRKDFDKVKQIMNLRI", "aa_hexdigest": "d3969568ee1c60af6e60a2100fd3071a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8568.956099999996, "isoelectric_point": 5.485781288146971 }, "id": "AHCKFKLKGB_1120", "locus": "GCA013367975_01119" }, { "type": "cds", "sequence": "contig_1", "start": 649642, "stop": 649968, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTAAATGAAACCGGACATTCAAAATACGCTTTTGTTTCGGCATGTTTAACAATGCTAACAAATGCTTCAATTAATTCTATTTGTTTATATGGATTTCACAAACCTGCATATTATGCTGCTTTTGGTTCAATTATTGCTGCGGCAGTTTGCTTTACAAGTGATTCTTTATTTACATATTACAAAGAAAGAAAAATTTTTATTAATGTATTTAAATTGTTTCATATTACAAAACCAATTGCAAAGCAATTATTAAAAAGAATTCCCGCCATGTTAATTGCCATTGCTGGAATGTTAACTTTACCTACAAGAATGATTATTTGA", "aa": "MLLNETGHSKYAFVSACLTMLTNASINSICLYGFHKPAYYAAFGSIIAAAVCFTSDSLFTYYKERKIFINVFKLFHITKPIAKQLLKRIPAMLIAIAGMLTLPTRMII", "aa_hexdigest": "dea4ca7d7358ced9b48335dafbb9f039", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12088.445999999982, "isoelectric_point": 9.676914024353028 }, "id": "AHCKFKLKGB_1121", "locus": "GCA013367975_01120" }, { "type": "cds", "sequence": "contig_1", "start": 650023, "stop": 650526, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTAATGCTGTTACAATACTTGGTTTAGTCGAAAGCCTTTCATCCGTCGCCTCGGCAATAACTTCTGTTTGTGCAAGCAATGTTTCATTTTTTGTTGCCACAAGATTAGGAGAATCTGATTTTGAAGGTGCAGATAAGCACGCCCATTCATTGCGGGGTTTTCATGCTTTGGCTGGATGTTGCATGTCTGTATTAATGATTGCGGTTGTTTTTGGAATTGCATATTCTCCTACTACAACAAAAGGTGTTGGTATTGGTGTCTCTCAAAGATTTTATGATGTGCAAAGAAAAGATTACATTATTGAAATATTAAATAAATCTGATATTTTAAAAAATAGTGATTTATTAAATTCACTTGGCAATGATTTTACATTCACAAAAGGTCAAAATTTGGCTTATGATCAATGGGTGGCAAGCGCAGTACTGGCAACTTCAAAAGAATTCAAAGAAACATTTTTGTTATGTAGTGCAACCTTTATTTTCTTTAACCCTATATGA", "aa": "MGINAVTILGLVESLSSVASAITSVCASNVSFFVATRLGESDFEGADKHAHSLRGFHALAGCCMSVLMIAVVFGIAYSPTTTKGVGIGVSQRFYDVQRKDYIIEILNKSDILKNSDLLNSLGNDFTFTKGQNLAYDQWVASAVLATSKEFKETFLLCSATFIFFNPI", "aa_hexdigest": "721e79c2fc00c8cdb6a0f53ad1a61d10", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18010.47039999999, "isoelectric_point": 5.85478000640869 }, "id": "AHCKFKLKGB_1122", "locus": "GCA013367975_01121" }, { "type": "cds", "sequence": "contig_1", "start": 650898, "stop": 651203, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00561.26" ], "nt": "ATGTTATATGATTTAGTTTCACCATATAATTCAGGACATTTAAAGGTTAGTGATATTCATAGTATTTATTATGAAGAATCAGGAAATCCAAATGGTAAGCCAATTTTGTTTGTCCATGGTGGTCCAGGTGGAGGAACCAAACCTTCATGTCGTCAATTTTTTGACCCTGAATTTTATAGAATTATTTTATTTGATCAAAGAGGATGTGGACTTAGCATACCTAGCGCCGAAATTAAACAAAATACAACTCAAGATTTAATAGAAGATATTGAAAAATTACGGAAATTTTTAAACATTGACAAGTGA", "aa": "MLYDLVSPYNSGHLKVSDIHSIYYEESGNPNGKPILFVHGGPGGGTKPSCRQFFDPEFYRIILFDQRGCGLSIPSAEIKQNTTQDLIEDIEKLRKFLNIDK", "aa_hexdigest": "049e0eec9caeeee4c69317414b102aa2", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A1L4BUW4", "query_cov": 0.9702970297029703, "subject_cov": 0.9245283018867925, "identity": 0.633, "score": 138.0, "evalue": 1.23e-40, "valid": false, "product": "Proline iminopeptidase", "uniref50_id": "UniRef50_A0A1L4BUW4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00561.26", "name": "Abhydrolase_1", "length": 71, "aa_cov": 0.6831683168316832, "hmm_cov": 0.9859154929577465, "evalue": 1.7545117909127412e-10, "score": 36.224388122558594, "start": 33, "stop": 101 } ], "seq_stats": { "molecular_weight": 11419.813099999985, "isoelectric_point": 5.849380302429199 }, "id": "AHCKFKLKGB_1123", "locus": "GCA013367975_01122" }, { "type": "cds", "sequence": "contig_1", "start": 651327, "stop": 651491, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATCAAAAAGGCGCTTCTTATTATTTTCCTGCTGAATATGAAGAATATTCAAACTTTATCCCTGAAAATGAAAGAGGCGATTTAATTAATGCTTATTATAAATATTTAAATTCAAGTGACCAAAATGTTGCAGAGCAAGCAGCATATCATTGGAGCAAATGA", "aa": "MYQKGASYYFPAEYEEYSNFIPENERGDLINAYYKYLNSSDQNVAEQAAYHWSK", "aa_hexdigest": "c869a2f5c7a6442840797d1be22a2af7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6457.877200000004, "isoelectric_point": 4.591988182067872 }, "id": "AHCKFKLKGB_1124", "locus": "GCA013367975_01123" }, { "type": "cds", "sequence": "contig_1", "start": 651510, "stop": 651752, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGTTACCTAATTTAGAAGAAATTTTATCCGATAAAAAAAGTAATTTAGAATTAGCAAGATTAGAAAATTATTATTTTGTTAATAAGATATTTTTAAATGATGATAATTTTATTTTAAATAACGCAAGTAAAATCAAAGAAATTCCAACGATATTAATTCATGGTCAATTTGATATGGATTGTCGCCCAGTTGGCGCATATTTATTGCATAAAGCCTTACCAAAATCAAAGCTTTGA", "aa": "MKKLPNLEEILSDKKSNLELARLENYYFVNKIFLNDDNFILNNASKIKEIPTILIHGQFDMDCRPVGAYLLHKALPKSKL", "aa_hexdigest": "8eff35bcba50ad8ab78d3385e415356d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9289.797099999992, "isoelectric_point": 8.734515190124512 }, "id": "AHCKFKLKGB_1125", "locus": "GCA013367975_01124" }, { "type": "cds", "sequence": "contig_1", "start": 651929, "stop": 652474, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09371.15" ], "nt": "ATGAATTCAATAAATAACGTTGCTAAAAAATTAAATTTAAGCGAAAATCAAGTAAAAAATACATTATCTTTGTTATCTGAAGGGGCAACAGTACCATTTATTTCAAGATATAGGAAAAATATAACTGGTGGTTTAGACGAAGATCAAATTACAAAAATAAATGATTTATATGTGTACGACGTTGAATTAAATAAAAGAAAAGAAGCCATATTAAATATATTGGCCGAAAATAAATTATTAAATGATGAATTAAAGCAAAAAATTGAAGCTGCTGATACAAAACAAGCAGTTGAAAATATTTATGAACCATTTAAAATAGGTAAAAAAACAAAAGCATCAGATGCTATTGCTCTTGGTTTAAGTCCATTGGCTGATGAAATTTTAAATAATAATGATGACAACTATAATCTTTATTATGCTGCTAAGAAATATATTAGTGATAAATTAAAAACTGAAGATGAAGTCTTACAACAAACTAAATTTATCATTGCTCAAAATATTTCTCAAGACCCTTCTACAAGAGAATATGTAAAAAATCAATTATGA", "aa": "MNSINNVAKKLNLSENQVKNTLSLLSEGATVPFISRYRKNITGGLDEDQITKINDLYVYDVELNKRKEAILNILAENKLLNDELKQKIEAADTKQAVENIYEPFKIGKKTKASDAIALGLSPLADEILNNNDDNYNLYYAAKKYISDKLKTEDEVLQQTKFIIAQNISQDPSTREYVKNQL", "aa_hexdigest": "fda9b6997e32972ffd4ac1a74bff54fa", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09371.15", "name": "Tex_N", "length": 73, "aa_cov": 0.40331491712707185, "hmm_cov": 1.0, "evalue": 1.9041000004792308e-29, "score": 96.57521057128906, "start": 2, "stop": 74 } ], "seq_stats": { "molecular_weight": 20543.994799999997, "isoelectric_point": 5.389893913269043 }, "id": "AHCKFKLKGB_1126", "locus": "GCA013367975_01125" }, { "type": "cds", "sequence": "contig_1", "start": 652748, "stop": 652987, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16921.10" ], "nt": "ATGCCTTCGATAATTAGAGAAATTAAAACCGAATTATTTGAAAGGGCTGAGGCAGAAGCTATTAAAGTATTTGCTTCAAACGTTGAACAAATGTTATTGAGTCCAGCAACTAAAAATAAAAGAATTTTAGCAATAGACCCTGCTTATGTTAATGGATGTAAAATTGCAGCCATTGATCAAAATGGTAACTTTTTAAAAGAAGGAATAATTTATCCTCATAATCACAATCAAGAACAATGA", "aa": "MPSIIREIKTELFERAEAEAIKVFASNVEQMLLSPATKNKRILAIDPAYVNGCKIAAIDQNGNFLKEGIIYPHNHNQEQ", "aa_hexdigest": "fc249100f8a5e0c5a4ba0b0df100781a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16921.10", "name": "Tex_YqgF", "length": 38, "aa_cov": 0.4810126582278481, "hmm_cov": 1.0, "evalue": 1.6809163409129456e-12, "score": 43.23159408569336, "start": 40, "stop": 77 } ], "seq_stats": { "molecular_weight": 8895.121799999999, "isoelectric_point": 6.0693471908569325 }, "id": "AHCKFKLKGB_1127", "locus": "GCA013367975_01126" }, { "type": "cds", "sequence": "contig_1", "start": 653099, "stop": 654076, "strand": "+", "frame": 2, "gene": null, "product": "S1 RNA-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A502M9J9" ], "nt": "TTGCAAAAAAGACTTGAAAAAAATCCTAATGAAAAAATTAAGTTTGTAATTGTAAGTGAAGTTGGCGCTTCTGTTTATTCAGCTTCAAAAATTGCCCAAGAAGAATTTCCACAATTACATGTTGAATATAGGTCAGCAATTCATATTGGTAGAAAGTTCCAAGATCCATTAAATGAATTAATTAAAATAGATCCTAAATCAATTGGAATAGGACAATATCAACATGATGTTAACCAAAAGGAATTATCAAAACAATTAACCGAAAAATTAAATAAAGTAGTTAACCTAGTTGGTGTTGATCTAAATACAGCAACAAAAGTGATTTTATCTTATATATCAGGATTAAGTAACACCATAGCAAGCAATATTGTAAACTATAGAGAAGAAAATGGTTCATTTAAATCACGTGAAGAACTTAAAAAAGTGAAAGGACTTGGGCCAAAAGCATACGAACAATCAGTTGGATTTTTAAGAATACATGATTCTAATAATTTTTATGACAAGACTAATATTCACCCCGAAAGTTATAAATTAGCAGATACCATTGTCAATAAGCTAAATATAGATTTAAATAATATTGACAAAGAAAAATTATTATCCGTTGACAAAGAACAACTTGCAAAAGAATTGAATTCTGATAAATACAGTATTCAACTTATTATAGAATCATTGATTGCACCCGAAAAAGATATTAGAGATGACAAACCAGGATTTTTAGTATCAGATAAAGTCTTAACTATTGATGATATAACTCAAGGTCAAATAATTAAGGGCCAAATTCAAAATGTTACCGATTTTGGTGCCTTTGCGTTTATTGGATTAAAACAAAACGTTTTGATCCATATAACCAATATGAAGAAGAAAGAAAATCAATTTATTAAACACCCTTTAGATGTATTAAATGTTGGTGATAATGTAAACATACAAATAATTTCCATTGATAAAGAACACAATAAAATTCAAGGAAAAATTATTTGA", "aa": "MQKRLEKNPNEKIKFVIVSEVGASVYSASKIAQEEFPQLHVEYRSAIHIGRKFQDPLNELIKIDPKSIGIGQYQHDVNQKELSKQLTEKLNKVVNLVGVDLNTATKVILSYISGLSNTIASNIVNYREENGSFKSREELKKVKGLGPKAYEQSVGFLRIHDSNNFYDKTNIHPESYKLADTIVNKLNIDLNNIDKEKLLSVDKEQLAKELNSDKYSIQLIIESLIAPEKDIRDDKPGFLVSDKVLTIDDITQGQIIKGQIQNVTDFGAFAFIGLKQNVLIHITNMKKKENQFIKHPLDVLNVGDNVNIQIISIDKEHNKIQGKII", "aa_hexdigest": "d0b90ef213ea410aacde4e8011e64be0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003593DDCE", "query_cov": 0.8615384615384616, "subject_cov": 0.9929078014184397, "identity": 0.7390000000000001, "score": 412.0, "evalue": 1.23e-142, "valid": false, "product": "helix-hairpin-helix domain-containing protein", "uniref50_id": "UniRef50_A0A502M9J9" }, "pscc": { "uniref50_id": "UniRef50_A0A502M9J9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A502M9J9" ], "product": "S1 RNA-binding domain-containing protein" }, "genes": [], "id": "AHCKFKLKGB_1128", "locus": "GCA013367975_01127" }, { "type": "cds", "sequence": "contig_1", "start": 654302, "stop": 655129, "strand": "+", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "nt": "ATGTTTTATAGATATATTTCTGAAAATATTGCATCATATGCAAACAAGCGCCAGATTCAAGCAGGAATCGAAGACTTTGATTATGCAACTTTATCAGATGAAGAAGCATTGACTGGCAAAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTGCCTTCTGAATTGTTTGTTAATGTTGTTAAAAATGCTACAACAAATAATTGTTTAAATGAAACGTTGGACAATATTTTTAAAAATATTGAATCATCAGCAAAAGGACAACAAAGTGAAAATGATTTTTCAGGTCTTTTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTACAAGAAATAGCAGCCATGAAATTAGGCAATTACCAAGATAATTCAATAGATGCCTTTGGCGATGCTTATGAATATTTGATGTCAATGTATGCATCAAATGCCGGAAAATCAGGGGGTGAATATTTTACTCCTCAAGAAGTTTCAGAATTACTTACTAAAATAGCAGTTTTTAATAAGAAAAAAGTTAATAGAGTATATGACCCTGCATGTGGATCGGGCTCGTTACTTTTACAAACAATCAAGGTTCTTGGCAAAGAAAACATCAAGGATGGATTTTATGGCCAAGAAGTCAATTTAACAACATACAACTTATGTAGAATAAATATGTTTTTGCATGATATAGGATTTGATAAATTCAATATTTATAACGGCGACACATTACTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTTATTGTTTCAAATCCTCCTTATTCAATTAAATGA", "aa": "MFYRYISENIASYANKRQIQAGIEDFDYATLSDEEALTGKDDLINEKGFFILPSELFVNVVKNATTNNCLNETLDNIFKNIESSAKGQQSENDFSGLFNDVDVNSQKLGRSVDERNKKLAAILQEIAAMKLGNYQDNSIDAFGDAYEYLMSMYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIK", "aa_hexdigest": "4362752380f9498a8a96a43a5ac6812e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0028EAAB7E", "query_cov": 1.0, "subject_cov": 0.8136094674556213, "identity": 0.655, "score": 364.0, "evalue": 1.27e-123, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7U9ST90", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9ST90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "AHCKFKLKGB_1129", "locus": "GCA013367975_01128" }, { "type": "cds", "sequence": "contig_1", "start": 655286, "stop": 655750, "strand": "+", "frame": 2, "gene": null, "product": "type I restriction-modification system subunit M", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0009307", "KEGG:K03427", "SO:0001217", "UniRef:UniRef50_A0A7X6YKI2", "UniRef:UniRef90_UPI001939D1E0" ], "nt": "ATGTATCGCGGGGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAAGCAACTTGTTTTTTGGAACAAGTATTTCAACATGCATAATGGTATTGAAAAAATCAAAAATTGACAACAATATCTTATTTATTGACGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAACAAATTAACTAGTCAAAACATTAACAATATTATTGATTATTATGGCCAAAGAAAAGACATTTCTTATATTTCTAAATTAGCCAGTGTTGAAGAAATTAAGTCCAATTCATATAATTTATCAGTCAATAGTTACATTGAAAAACAAGACACTTCAGAAAAAATAGATATTAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAACATTGAGAAGCGAAATTGACAAAATAGTTGCTGAATTAGAGGAATAG", "aa": "MYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQRKDISYISKLASVEEIKSNSYNLSVNSYIEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE", "aa_hexdigest": "830d37e0255a6f4822ad84ed29f54a19", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_R6V7S9", "query_cov": 0.9935064935064936, "subject_cov": 0.9807692307692307, "identity": 0.654, "score": 199.0, "evalue": 3e-63, "valid": false, "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A645HPD1" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 654986, "stop": 655750, "observations": { "mutation": [ 655127, 655238 ], "stop": [ 655127, 655238 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939D1E0", "query_cov": 1.0, "subject_cov": 0.4652567975830816, "identity": 0.987, "score": 281.0, "evalue": 3.71e-93, "gene_start": 1, "gene_end": 154, "reference_start": 178, "reference_end": 331, "reference_sequence": "MYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIKWEGEDNPLLINDQRFSPAGILAPKSKADFAFILHSLSWLATDGVAAIVCFPGIMYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQWKDISYISKLASVEEIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE" }, "paralog": false, "identity": 0.984251968503937, "score": 488.0, "evalue": 4.28e-177, "description": "Internal stop codon at 655127, 655238. Nonsense mutation around 655127, 655238.", "psc": { "uniref90_id": "UniRef90_UPI001939D1E0", "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7X6YKI2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939D1E0", "EC:2.1.1.72", "UniRef:UniRef50_A0A7X6YKI2", "KEGG:K03427", "GO:0003677", "GO:0009007", "GO:0009307" ] } }, "id": "AHCKFKLKGB_1130", "locus": "GCA013367975_01129" }, { "type": "cds", "sequence": "contig_1", "start": 655879, "stop": 656457, "strand": "+", "frame": 1, "gene": null, "product": "Type I restriction enzyme S protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR48" ], "nt": "TTGTCGTTTAAGCATGTTTCTGCAAATGAACTCAAGCTCTATAAAAAAGAAGAAACGGGAAATGTAAAATTATTATCAACAGGCCAATATGATGGTTTTACCTCCTATGTTGAAAATGATAAAAACATTAATTATGGTGAGATTATTTCAATACCATCTGGTGGCGCGCCATTAATAAAATACTACAAAGGATATTTTATTGATAGTTTAAATATATTATTATCATCAAAAGATGTTAAAAAATATAGTCTCAAATTCTTATATTACTTTCTAATTGCTAACAAGGAAAGCATTGAACAAAATTTTAGGGGTTCCTCAATAAAACACCCTGATATGGAAAATATTATCGAGTTGCAAATTCCGCTTCCCCCTCTTGAAATTCAAAATGAAATTGTTAATATTTTAGACAAGTTCACTGAGTTGACCACCGAGTTGACCACCGAGTTGACCTATAGAGATAAACAATATAATTACTATAGAAATAAATTACTAGACTTTGATAATAATAAAGAATTATTAAACAAGATAATGAATAATCAACAATGTAATAATAGTATAGTTGAATATAAGAGGTTATGA", "aa": "MSFKHVSANELKLYKKEETGNVKLLSTGQYDGFTSYVENDKNINYGEIISIPSGGAPLIKYYKGYFIDSLNILLSSKDVKKYSLKFLYYFLIANKESIEQNFRGSSIKHPDMENIIELQIPLPPLEIQNEIVNILDKFTELTTELTTELTYRDKQYNYYRNKLLDFDNNKELLNKIMNNQQCNNSIVEYKRL", "aa_hexdigest": "01625a95661649318064432174e8ec9d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PR48", "query_cov": 0.8854166666666666, "subject_cov": 0.9826589595375722, "identity": 0.6409999999999999, "score": 218.0, "evalue": 7.95e-70, "valid": false, "product": "Type I restriction enzyme S protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q9PR48", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_Q9PR48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR48" ], "product": "Type I restriction enzyme S protein" }, "genes": [], "id": "AHCKFKLKGB_1131", "locus": "GCA013367975_01130" }, { "type": "cds", "sequence": "contig_1", "start": 656518, "stop": 657090, "strand": "+", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00131480C8", "UniRef:UniRef90_UPI00131480C8" ], "nt": "TTGTCATTTAAGCATGTTTCCGCAAATGAACTTAAGCTCTATAAAAAAGAAGAAACGGGAAACGTAAAATTATTATCAACAGGTCAATATGATGGTTTTACCTCCTATGTTGAAAATGATAAAAACATTAATTATGGTGAGATTATTTCAATACCATCTGGTGGCGTGCCATTAATAAAATACTACAAAGGATATTTTATTGATAGTTTAAATATATTATTATCATCAAAAGATGCTAAAAAATATAGTCTTAAATTCTTATATTACTTTTTAATTGCTAACAAGGAAAACATTGAACAAAATTTTAGGGGTTCCTCAATAAAACACCCTGATATGGAAAATATTATTGAATTGCAAATTCCACTCCCCCCTCTTGAAATCCAAAATAAAATAGTTGAAATCCTTGACAAACTTGAAACATATACCAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTTTGA", "aa": "MSFKHVSANELKLYKKEETGNVKLLSTGQYDGFTSYVENDKNINYGEIISIPSGGVPLIKYYKGYFIDSLNILLSSKDAKKYSLKFLYYFLIANKENIEQNFRGSSIKHPDMENIIELQIPLPPLEIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGGVLSKNYLLLLNEL", "aa_hexdigest": "70797389319f85d86b45e683216016bf", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00131480C8", "query_cov": 0.9263157894736842, "subject_cov": 0.8036529680365296, "identity": 0.972, "score": 343.0, "evalue": 1.67e-118, "valid": true, "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00131480C8", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_UPI00131480C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00131480C8" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "AHCKFKLKGB_1132", "locus": "GCA013367975_01131" }, { "type": "cds", "sequence": "contig_1", "start": 657133, "stop": 657282, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGTGTGAAATAAAGCGAGGAAAAGTTTACTCAAAAGAATTCATAAAATTAAATAAAGGTGAATACCCTGTTTATTCATCTCAAAGTTTAAACGATGGAATTTTAGGAAAAATTGACAAATATGATTTTGATGGTGAATATGTAACATGA", "aa": "MCEIKRGKVYSKEFIKLNKGEYPVYSSQSLNDGILGKIDKYDFDGEYVT", "aa_hexdigest": "b0f70c5bb8111e7c075e0d3502c6a325", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 53, "aa_cov": 1.0, "hmm_cov": 0.9811320754716981, "evalue": 1.936251701824757e-09, "score": 33.23988342285156, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 5670.380600000001, "isoelectric_point": 6.121525382995604 }, "id": "AHCKFKLKGB_1133", "locus": "GCA013367975_01132" }, { "type": "cds", "sequence": "contig_1", "start": 657415, "stop": 657933, "strand": "+", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_D1J8W0", "UniRef:UniRef90_UPI00273707BF" ], "nt": "ATGCAGACAAAAAAATTTGTAAACAAGGCAAGCGGAAATCCTAAACTCATGAGTAATATTATGGAAAATATTGAAATCCCTATCCCTCACATTTCCATCCAAAATAAAATTGTTGAAATCCTTGACAAACTTGAAACATATACAAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGAAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTACATATTATTATTAAATGAACTTTATGAAAAGATAATTAACATTATTGAATATAAGAGAATGAACGAGGTAACTATTAATTTAAAAAAGGAAACATTAGAAAAAAACAAATTACTAAATAATGGCAAATATCAAGTAATTAATGGTGGCAAAGAAATATATGGGACATATAACCAATATAATAATGAAGGAAATACTATAACAATTGCAGCGCGTGGAGCTGCCGGTTTTGTAAATTATATGAATGATAAATTCTGA", "aa": "MQTKKFVNKASGNPKLMSNIMENIEIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGVLSKNYILLLNELYEKIINIIEYKRMNEVTINLKKETLEKNKLLNNGKYQVINGGKEIYGTYNQYNNEGNTITIAARGAAGFVNYMNDKF", "aa_hexdigest": "98644bac030bffd9b03c1cd0153431f1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 657115, "stop": 658236, "observations": { "mutation": [ 657280, 657931 ], "stop": [ 657280, 657931 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00273707BF", "query_cov": 1.005813953488372, "subject_cov": 0.4199029126213592, "identity": 0.9420000000000001, "score": 320.0, "evalue": 9.71e-107, "gene_start": 1, "gene_end": 172, "reference_start": 127, "reference_end": 299, "reference_sequence": "MRGGVLSKNYLLLLNELWDKIVNIVEYDLIKNLCEIKRGKVYSKEFIKLNKGEYPVYSSQSLNDGILGKIDKYDFDGEYVTWTTDGAYAGTVFYRIGRFSITNVCGILSVLNKSKLNVKYLSTCLSMQTKKFVNKASGNPKLMSNIMENIEIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGVLNNNYILLLNKLYEKIINIIEYKRINEATINLKKETLAKNKLLNNGKYQVINSGKEIYGTYDQYNNEGNTITIAARGAYAGFINYMNDKFWAGGLCYPYRSKNETSFLTKYIYYWLKYNEEKISNELVAKGSIPALNKIDIDNYFIPIPHISIQNKIVEILDKLQAYTKDIQTGLPLEIDQRKKQYEHYRDKLLNFKEKNTKI" }, "paralog": false, "identity": 0.9545454545454546, "score": 716.0, "evalue": 1.73e-265, "description": "Internal stop codon at 657280, 657931. Nonsense mutation around 657280, 657931.", "psc": { "uniref90_id": "UniRef90_UPI00273707BF", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_D1J8W0", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00273707BF", "EC:3.1.21.3", "UniRef:UniRef50_D1J8W0", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8W0" ], "product": "Type I restriction enzyme specificity protein" } }, "id": "AHCKFKLKGB_1134", "locus": "GCA013367975_01133" }, { "type": "cds", "sequence": "contig_1", "start": 657999, "stop": 658097, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTATCAATATCAATTTTATTTAATGCCGGAATGCTACCTTTTGCAACTAATTCATTTGATATTTTTTCTTCATTATATTTAAGCCAATAG", "aa": "MKKLSISILFNAGMLPFATNSFDIFSSLYLSQ", "aa_hexdigest": "64cfd04191ad04be216fe0c03a641ad2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3585.1929, "isoelectric_point": 8.253838920593264 }, "id": "AHCKFKLKGB_1135", "locus": "GCA013367975_01134" }, { "type": "cds", "sequence": "contig_1", "start": 658304, "stop": 658543, "strand": "+", "frame": 2, "gene": null, "product": "Type I restriction-modification system, restriction subunit R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H0F8" ], "nt": "ATGGAAAAATATAACATTATTGTTGAAAACGAGAATGATACGGTTGTTTCTGAATATAAGCCTCTAGCGAATAAAGCAAAAGATTATCAAACAGAAAGTGAACTTGAAAGTCAATTCATATCAATGCTTAAAAACCAAGGATATGAGTATTTGAACATTCATAATGAAGAAGATTTAATTAATAATTTAAGACAACAAATTGAATTGTTAAATAAAATAAATTTTTCAGATAATGAATGA", "aa": "MEKYNIIVENENDTVVSEYKPLANKAKDYQTESELESQFISMLKNQGYEYLNIHNEEDLINNLRQQIELLNKINFSDNE", "aa_hexdigest": "cc1b22fe18b7ae4c95a225b4c13a441b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A0F5H0F8", "query_cov": 1.0379746835443038, "subject_cov": 1.0, "identity": 0.524, "score": 71.2, "evalue": 1.14e-14, "valid": false, "product": "Type I restriction-modification system, restriction subunit R", "uniref50_id": "UniRef50_A0A0F5H0F8" }, "pscc": { "uniref50_id": "UniRef50_A0A0F5H0F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H0F8" ], "product": "Type I restriction-modification system, restriction subunit R" }, "genes": [], "id": "AHCKFKLKGB_1136", "locus": "GCA013367975_01135" }, { "type": "cds", "sequence": "contig_1", "start": 659321, "stop": 660415, "strand": "+", "frame": 2, "gene": null, "product": "DEAD/DEAH box helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3E2CM26" ], "nt": "TTGCCTTATATAAATAAAGTTATTTTCGTTGTTGATAGAAGAGATTTGGACTATCAAACAATGAAGGAATATGACAAATTAGAAAAAGGCGCTGCTAATTCAAATCGTTCTACATCAATATTGCAAAGACAACTAGAATCTAATGACCCTAATAAAAAAATAATAATTACAACTATTCAAAAATTATCAATTTTTATAAAAAATAATAGAAATCATCCTGTTTTTGATAAAGAAGTTGTTTTAATATTTGATGAATGTCACCGCTCTCAATTTGGTTCAATGCATACAGCTGTTATTAAAAATTTTAAAAAATATTATTTATTTGGTTTTACCGGAACACCAATTTTTGCAGCTAATATCAATAAATCAAGTCCGGAAGTGATTAAAACAACTGAACAAGCTTTTGGCCAAAAATTACATACTTATACAATAATAAATGCAATAAATGATAGAAACGTTTTGCCATTTAAAATTGAATATATTAAAACAATTGATGTTGATAAATCACTAAACAATAAAGAGGATCAAAAAGTAAAAGATATTGATCGGGAAAGTGTTTTTTTAGATCAAAGAAGAATTAAACTAGTTGTTGAATACATATTGAAACATTTCGATCAAAAAACTAGGAGAAATTCCAATGTAATTTATAGCCATTCAATATCTGATATTTTAGATAAAACTATTAAAAGAAATGTGAGCGGTTTTAATTCTATAATGGCGGTTTCTTCAATAGAAATGGCTAAAAAGTATTATTTAGAATTTAAAAACCAGCTTGCTCAAGACCCTAAAAACAATCTAAAAATTGGTTTAATATATTCATATGGGGTTAATGGCGAAGAATCATATGGTGGTTTAATCGATGAAGAGGATAATGAAAATACATTGCAATTAAATAAGCCAGATAGAGATTTTTTGGATGATGCTATTAAAGATTATAACAATATGTTCAACACAAACTTTAATAGTGAAGGTGAACAATTTCAAATGTATTATAAAGATGTTTCTTTAAAACTAAAAAATAAAGATTTGGACATGCTGATTGTAGTAAACATGTTTTTAACTGGCTTTGACGCCCCAACATTAAATACATTATGA", "aa": "MPYINKVIFVVDRRDLDYQTMKEYDKLEKGAANSNRSTSILQRQLESNDPNKKIIITTIQKLSIFIKNNRNHPVFDKEVVLIFDECHRSQFGSMHTAVIKNFKKYYLFGFTGTPIFAANINKSSPEVIKTTEQAFGQKLHTYTIINAINDRNVLPFKIEYIKTIDVDKSLNNKEDQKVKDIDRESVFLDQRRIKLVVEYILKHFDQKTRRNSNVIYSHSISDILDKTIKRNVSGFNSIMAVSSIEMAKKYYLEFKNQLAQDPKNNLKIGLIYSYGVNGEESYGGLIDEEDNENTLQLNKPDRDFLDDAIKDYNNMFNTNFNSEGEQFQMYYKDVSLKLKNKDLDMLIVVNMFLTGFDAPTLNTL", "aa_hexdigest": "39361065086144d011939f403bfa71f3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030771B9D", "query_cov": 1.0274725274725274, "subject_cov": 0.9280397022332506, "identity": 0.583, "score": 418.0, "evalue": 2.72e-142, "valid": false, "product": "HsdR family type I site-specific deoxyribonuclease", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A3E2CM26", "kegg_orthology_id": "K01153" }, "pscc": { "uniref50_id": "UniRef50_A0A3E2CM26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3E2CM26" ], "product": "DEAD/DEAH box helicase" }, "genes": [], "id": "AHCKFKLKGB_1137", "locus": "GCA013367975_01136" }, { "type": "cds", "sequence": "contig_1", "start": 660740, "stop": 661387, "strand": "+", "frame": 2, "gene": null, "product": "type I restriction endonuclease subunit R, EcoR124 family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A412PBS6", "UniRef:UniRef90_UPI00280A69CD" ], "nt": "TTGTTTGGTAAGGTATTAAAATTAAAAAATATTCTTGCTTCTTTTGATGAATTCATTGGTAAAGAAATTTTAAACGTTAGAGATTTTCAAAATTATTCAAGCAAATATTTGGATATAAAAGATGAATTTAAAAAGATATATAAAGAAGAAATTGCTTCAATTAACGATGATGTAATTTTTGAAACCGAATTATTAAAGACTTACGAAGTTAATATTGATTATATATTGCAAAAGGTAGAAGAGTTTAGAGGAAAGACAATTACTGAAATTAACAATTTAAGAAGTGAAATATCTTTAGCCTCTTCTTCATCGCCTGAATTAAGAAGCAAAAAACAATTAATTGAAAAATTCGTTTCAACAATTAATAATAATGAACTAGATATCCCAACTAAATTTCAAGAATTTATGGAACTAGAAAAGAAGAAGGAACTTGACAAAATTATTAGTGAAGAAAATTTAGATTTTGATAAAACCCATAAATTTATGGATAATTCGTTTGAGTTAGGAGAAATAAAAACTGCTGGTAGTGAGATTGGAGATTTATTGCCTAAAATGCCATTATTTGGTTCAAAAGGAAAAGAAAGATCTATTAAAAAGAAGTCAGTTTTAGAAAAATTTGAAAATTATTTCCAAAAATTTTATATTTAA", "aa": "MFGKVLKLKNILASFDEFIGKEILNVRDFQNYSSKYLDIKDEFKKIYKEEIASINDDVIFETELLKTYEVNIDYILQKVEEFRGKTITEINNLRSEISLASSSSPELRSKKQLIEKFVSTINNNELDIPTKFQEFMELEKKKELDKIISEENLDFDKTHKFMDNSFELGEIKTAGSEIGDLLPKMPLFGSKGKERSIKKKSVLEKFENYFQKFYI", "aa_hexdigest": "928e092298aea0f79de3a6ba82e8bee2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 660440, "stop": 661387, "observations": { "mutation": [ 660698 ], "stop": [ 660698 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280A69CD", "query_cov": 1.0, "subject_cov": 0.5456852791878173, "identity": 0.981, "score": 400.0, "evalue": 5.86e-138, "gene_start": 1, "gene_end": 215, "reference_start": 180, "reference_end": 394, "reference_sequence": "MQLNKPDRDFLDGAIKDYNNMFNTNFNSEGEQFQMYYKDVSLKLKNKDLDMLIVVNMFLTGFDAPTLNTLWVDKKLREHGLIQAFSRTNRILNDKKVYGNIVCFRNLEKETDQAISIFGDGEASGIVKIKNFVDYYYGYNDSQQVHHNGYCDYIQKLNNEFPLDNWNVISEQKNKEFIILFGKVLKLKNILASFDEFIGKEILNVRDFQNYSSKYLDIKDEFKKIYKEEIASINDDVIFETELLKTYEVNIDYILQKVEEFRGKTITEINNLRNEISLASSSSPELRSKKQLIEKFVSTINNNEQDIPTKFQEFMELEKKKELDKIISEENLDFDKTHKFMDNSFELGEIKTAGSEIGDLLPKMPLFGSKGKERSIKKKAVLEKFENYFQKFYI" }, "paralog": false, "identity": 0.9841269841269841, "score": 614.0, "evalue": 7.84e-225, "description": "Internal stop codon at 660698. Nonsense mutation around 660698.", "psc": { "uniref90_id": "UniRef90_UPI00280A69CD", "product": "type I restriction endonuclease subunit R, EcoR124 family", "uniref50_id": "UniRef50_A0A412PBS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280A69CD", "UniRef:UniRef50_A0A412PBS6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A412PBS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A412PBS6" ], "product": "Type I restriction endonuclease subunit R" } }, "id": "AHCKFKLKGB_1138", "locus": "GCA013367975_01137" }, { "type": "cds", "sequence": "contig_1", "start": 661451, "stop": 662077, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "ATGCCTCCCTTTTTACTTAAAATAGATAAAAAAGGAATGAAAATGAGTGAAATAAAATTAACAGCAAAAGAAATTTACAAACAAATTTATAACCTTTCACAAATTAAAAAAATAGATCAAGCAGAGGTTGTTGAAGCTTTTAAAAATACAGTTACAAAGTTAATAACTGAAACTTATGACGAAGAAGCAGATCTTGAATTTATTTTCGATCAAGAAAATGACAATTTTGCAATTATTAATCATAATAAAGTTGTCGTTGGCGATCCAATAACAAGCGAAGAAAAAGATCGCTTAACAAGATGCATAGAAATACCATTAAGTGATGCTAAAAAAATAGATCCAAATGTTGAAATTGGAGATTCTCTTTCTCAAGAAATTAACTTTGAATATTTTAGTAAAAAAGACTATAACAAAATTCTTGCTAATTTCAGTCAAGACATTAAAAATTTAGAAAGAAAAATGACTTGTGAAAAATATGCAAGTGAAGTTGGAAATTCAACTAAGGCAAAAATTGTTTCTGTTAACAAAGGAAAAGTAAATCTTGAATTAAGAGACGGAACTTTGGCATTTATGCCTTCTAATTTTGTAAATCAAAGAATAATTTCAAAATTAAATCCAGGTGATTGA", "aa": "MPPFLLKIDKKGMKMSEIKLTAKEIYKQIYNLSQIKKIDQAEVVEAFKNTVTKLITETYDEEADLEFIFDQENDNFAIINHNKVVVGDPITSEEKDRLTRCIEIPLSDAKKIDPNVEIGDSLSQEINFEYFSKKDYNKILANFSQDIKNLERKMTCEKYASEVGNSTKAKIVSVNKGKVNLELRDGTLAFMPSNFVNQRIISKLNPGD", "aa_hexdigest": "fa2b76befdc2bab8bbaee7ee6eb46e62", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 130, "aa_cov": 0.625, "hmm_cov": 0.9153846153846154, "evalue": 4.833778650785177e-21, "score": 70.53514099121094, "start": 24, "stop": 153 } ], "seq_stats": { "molecular_weight": 23793.965500000017, "isoelectric_point": 5.478733253479004 }, "id": "AHCKFKLKGB_1139", "locus": "GCA013367975_01138" }, { "type": "cds", "sequence": "contig_1", "start": 662285, "stop": 663091, "strand": "+", "frame": 2, "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "db_xrefs": [ "COG:COG0195", "COG:K", "SO:0001217", "UniRef:UniRef50_D1J8W2", "UniRef:UniRef90_D1J8W2" ], "nt": "ATGGAAGAAATCGGCGCAATAATGGGACGTGATTCTGAAAGAATTTCAACAATATCTAGAAAACTTAATGGTGAAAAAATAGATGTAGTTTTATATTCAGATGATATAAAAGAATATATTAAAAATGCATTAAGTCCAGCAAAGGTTATTGATTTAATTGAAGTTCCATCAAAAAATTCTTATCCTTCATTTAATGTTATTGTTCCAACAATTCAACATACGCTTGCTATTGGAAAGAAAGGCCAAAATGTTTCTTTAGCTTCTGAATTAGTAAAAGCAAAATTAGATATTTTAAGCCAAGAACAAGCAGACGAAAGAGGCATTGAATACAATTTTGAAAATGGTAATATTACAAAAGAAGAAATTGAATTACTTGAAAGTGGTAAAAAACTTCATCACTCATTTAAACACCCAAGAACTATTGGCAATAAAAATGCTACAAACAAATTTAATACGTTTGAAAATAGTTTTAATATTGATGAATTTGATGAAGACCTTGCCGAAATGAGAAGAAAAGCTCAACAAAATGATAATATCTTTGAAAGACAAATGTTTTCAAATAGTCTAGATGAAGATTTAGAAAGAACTTTATCAGAACTAAATGACGACAAAGAAAATAATGTTGATGAAAATGACCTTGATCCTTATTCAAAATCTTTTGATGAATTAAATGAACAAAAAAATGATGAAGAAGAGGATTATGAAAAAATTACAGCAACAAAAATGAAAGATTTTAAACGTGATGAAGATTTAAGCAGTGGATTAGAAAACATTGATTTAAGTGATTTGGATGATGAAAAATGGTAA", "aa": "MEEIGAIMGRDSERISTISRKLNGEKIDVVLYSDDIKEYIKNALSPAKVIDLIEVPSKNSYPSFNVIVPTIQHTLAIGKKGQNVSLASELVKAKLDILSQEQADERGIEYNFENGNITKEEIELLESGKKLHHSFKHPRTIGNKNATNKFNTFENSFNIDEFDEDLAEMRRKAQQNDNIFERQMFSNSLDEDLERTLSELNDDKENNVDENDLDPYSKSFDELNEQKNDEEEDYEKITATKMKDFKRDEDLSSGLENIDLSDLDDEKW", "aa_hexdigest": "a35c24dfad7c37351a139c1efe5c67ae", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusA" ], "truncated": "5-prime", "pseudogene": { "start": 661985, "stop": 663091, "observations": { "mutation": [ 662075 ], "stop": [ 662075 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W2", "query_cov": 1.0, "subject_cov": 0.4908424908424908, "identity": 0.993, "score": 501.0, "evalue": 9.46e-175, "gene_start": 1, "gene_end": 268, "reference_start": 279, "reference_end": 546, "reference_sequence": "MPPFLLKIDKKGMKMSEIKLTAKEIYKQIYNLSQIKKIDQAEVVEAFKNTVTKLITETYDEEADLEFIFDQENDNFAIINHNKVVVGDPITSEEKDRLTRCIEIPLSDAKKIDPNAEIGDSLSQEINFEYFSKKDYNKILANFSQDIKNLERKMTCEKYASEVGNSTKAKIVSVNKGKVNLELRDGTLAFMPSNFVNQRIISKLNPGDWIDVVIEEVKEENSNAQIIVSSVESRLLQKLFEIEIPEISQGLISIVNIARIPGERAKVSIKKSNDAPETMEEIGAIMGRDSERISTISKKLNGEKIDVVLYSDDIKEYIKNALSPAKVIDLIEVPSKNSYPSFNVIVPTIQHTLAIGKKGQNVSLASELVKAKLDILSQEQADERGIEYNFENGNITKEEIELLESGKKLHHSFKHPRTIGNKNASNKFNTFENSFNIDEFDEDLAEMRRKAQQNDNIFERQMFSNSLDEDLERTLSELNDDKENNVDENDLDPYSKSFDELNEQKNDEEEDYEKITATKMKDFKRDEDLSSGLENIDLSDLDDEKW" }, "paralog": false, "identity": 0.9918478260869565, "score": 711.0, "evalue": 7.28e-260, "description": "Internal stop codon at 662075. Nonsense mutation around 662075.", "psc": { "uniref90_id": "UniRef90_D1J8W2", "gene": "NusA", "product": "Transcription termination/antitermination protein NusA", "uniref50_id": "UniRef50_D1J8W2", "cog_id": "COG0195", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W2", "UniRef:UniRef50_D1J8W2", "COG:COG0195", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8W2" ], "product": "Transcription termination/antitermination protein NusA" } }, "id": "AHCKFKLKGB_1140", "locus": "GCA013367975_01139" }, { "type": "cds", "sequence": "contig_1", "start": 663085, "stop": 663342, "strand": "+", "frame": 1, "gene": "ylxR", "product": "YlxR domain-containing protein", "db_xrefs": [ "COG:AJ", "COG:COG2740", "KEGG:K07742", "SO:0001217", "UniRef:UniRef50_D1J8W3", "UniRef:UniRef90_D1J8W3" ], "nt": "ATGGTAAAGTCTATACAAAGAAAGTCAATTGTTGATAATAAAATATATGATATAGATTTATTGCTTAGATTTTCTAAAGATAAAAACAATTTAATTAGCTTTGATCCTTACAAAAAGAACCCCGGAAGAGGCGCATATTGTCTAAATGATAGTGAACAAATTGAAATTCTATTTAAAAGAAGATTATTAAATAAAGCATTTAAGCAGAATATAGATATTCAAAAATACAACGAAATAAGGAAAGAGGTGGACGAATGA", "aa": "MVKSIQRKSIVDNKIYDIDLLLRFSKDKNNLISFDPYKKNPGRGAYCLNDSEQIEILFKRRLLNKAFKQNIDIQKYNEIRKEVDE", "aa_hexdigest": "50e290183594512d6394b48159039ae9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8W3", "query_cov": 1.0, "subject_cov": 0.85, "identity": 0.988, "score": 168.0, "evalue": 9.64e-53, "valid": true, "gene": "YlxR", "product": "YlxR domain-containing protein", "uniref50_id": "UniRef50_D1J8W3", "cog_id": "COG2740", "cog_category": "AJ", "kegg_orthology_id": "K07742" }, "pscc": { "uniref50_id": "UniRef50_D1J8W3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8W3" ], "product": "YlxR domain-containing protein" }, "genes": [ "ylxR" ], "id": "AHCKFKLKGB_1141", "locus": "GCA013367975_01140" }, { "type": "cds", "sequence": "contig_1", "start": 663339, "stop": 664082, "strand": "+", "frame": 3, "gene": null, "product": "Translation initiation factor IF-2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357QY70" ], "nt": "ATGAGTAAACAAAATATCCAAAAACGAAAATCAAACGTTAGCTCATTAAAAGAGCAATTAACTGCTGGTGAAACAAAATTACAAGATGGCGTTTTTATATTTAATGGGCCATTGACAATTTCAGAATTCTCTCTTAAAATAAATAAAAACCCTACTGAAATATTGACCTATTATTTTAAAAAAGGAATAATCAAAACAATTAACACAACATTAAGCGAAGATGAAATAGCAGAACTTTGTTTTGAATTTAATTTAGATTTTAAAAAAGAAGAAAATGTTGATGCTCAAAATTTGTTTAATAAGCTTGAAATTGTTGAAGATCCAAATGATTTAGCTTCTAGACCCCCAATAGTAACAATTATGGGACATGTTGACCATGGTAAGACTACATTAATTGATAGAATAAGACATAGTAATGTTACTGAAACAGAATTCGGTGGTATTACTCAACACACCGGAGCATATCAAGTTGAATATCAAGGACGCAAAATTACATTTTTAGATACACCTGGTCATGAGGCTTTTACTGAAATGCGTTCAAGGGGAGCAAAAATTACTGATATAGTAGTCATTGTAGTTGCGGCAGATGATGGAGTTAAGCCCCAAACTATTGAAGCAATAGACCATGCAAAAGCTGCCAATGTGCCAATTATTGTTTTTGTTAATAAAATGGATAAGCAAGGAGCTGATATTGAAAGAATAAAAAGTGAACTTGCTGATAACAATGTTTTATGTGAAGAATGA", "aa": "MSKQNIQKRKSNVSSLKEQLTAGETKLQDGVFIFNGPLTISEFSLKINKNPTEILTYYFKKGIIKTINTTLSEDEIAELCFEFNLDFKKEENVDAQNLFNKLEIVEDPNDLASRPPIVTIMGHVDHGKTTLIDRIRHSNVTETEFGGITQHTGAYQVEYQGRKITFLDTPGHEAFTEMRSRGAKITDIVVIVVAADDGVKPQTIEAIDHAKAANVPIIVFVNKMDKQGADIERIKSELADNNVLCEE", "aa_hexdigest": "602c93818d65bf5a6bd2e13b8c4b98da", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0025CBB306", "query_cov": 1.0242914979757085, "subject_cov": 0.8322368421052632, "identity": 0.534, "score": 263.0, "evalue": 1.58e-84, "valid": false, "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_A0A357QY70", "kegg_orthology_id": "K02519" }, "pscc": { "uniref50_id": "UniRef50_A0A357QY70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357QY70" ], "product": "Translation initiation factor IF-2" }, "genes": [], "id": "AHCKFKLKGB_1142", "locus": "GCA013367975_01141" }, { "type": "cds", "sequence": "contig_1", "start": 664116, "stop": 665156, "strand": "+", "frame": 3, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413", "KEGG:K02519", "SO:0001217", "UniRef:UniRef50_Q98R05", "UniRef:UniRef90_D1J8W4" ], "nt": "TTGAAGAATACTAATATAAATGAATTACTTGAAGCAATATTTTTGCAAGCTGATTTACTTGATTTAAAGGCTAATATAAATAGAGATCCTATCGGAACAATTATTGAATCTAGAATTGACAAAGGACGTGGAGTTGTTTCGACTGTTATTGTTGAAAGTGGAACATTGTTACCTCGTGATTTCATTGTAGCTGGAGGGCAATATGGCAAGATTAGAAGTCTAACTTCTATTGATGGTAAGCCTATACAAAAAGCATTGCCTGGTACTCCATGTATAATTACTGGTTTAAGCAAAAACCCTCAATCGGGTGATAAATTTGTAGCTATTAGTGATGAAAAATTTGCTAAAAAATTAGCATCTGAAAAATCATATTTAGATAAATGTAAAGAATTAAATGAGAAAAATGCATTTTCTATACAAGATGGAATAAAAACTGTAAATGTCATTGTTAAGACAGATGTTCAAGGAACAGCAGAAGCAATTAAACAAACATTGCAAAAAATCAGAAATGATGAAATCGCAGTTAATGTTATAAGAGCAACCTCTGGCGAAGTAACAAAAGCAGATATTTTGCTTGCTGAAACTTCTAATGCAAGAATCTATTTATTCAATAATACAATAAATAACGAAATTAAAAAGGCTGCTGAATTAAAGAATATAAAAATCTTTAAACATAATATCATTTATAAAATGGTTGAAGAATTAGAAACTTTAATAACAACCTTAAAAGAACCTGTTTACGAAGAAAAATTAATTGGATCTGCAACCGTCCTACAACTATTCTTTTATTCAAAAATAGGTCAAATTGCAGGATGCATGATGCAAGAAGGCATTGTTAAGGAATTTAGTAAGGTCGAGGTATACAGACGCAATAAATTAATTTTTAAAGGACAAATTATTTCTCTAAAAAGAGGAAAAAATGATGTCAAATCTGTTGAAAAAGGTTTTGATTTTGGAACTCATGTCAAGGATTTTGATAAAATAGAAGTAGATGATGAATTAAAATTTTATGAGGATGTATTAGTCAATGAACACAATTAG", "aa": "MKNTNINELLEAIFLQADLLDLKANINRDPIGTIIESRIDKGRGVVSTVIVESGTLLPRDFIVAGGQYGKIRSLTSIDGKPIQKALPGTPCIITGLSKNPQSGDKFVAISDEKFAKKLASEKSYLDKCKELNEKNAFSIQDGIKTVNVIVKTDVQGTAEAIKQTLQKIRNDEIAVNVIRATSGEVTKADILLAETSNARIYLFNNTINNEIKKAAELKNIKIFKHNIIYKMVEELETLITTLKEPVYEEKLIGSATVLQLFFYSKIGQIAGCMMQEGIVKEFSKVEVYRRNKLIFKGQIISLKRGKNDVKSVEKGFDFGTHVKDFDKIEVDDELKFYEDVLVNEHN", "aa_hexdigest": "07112a52def80948ccf737551fbdaa22", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "infB" ], "truncated": "5-prime", "pseudogene": { "start": 663816, "stop": 665156, "observations": { "mutation": [ 664080 ], "stop": [ 664080 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W4", "query_cov": 1.0, "subject_cov": 0.571900826446281, "identity": 0.9940000000000001, "score": 659.0, "evalue": 1.74e-234, "gene_start": 1, "gene_end": 346, "reference_start": 260, "reference_end": 605, "reference_sequence": "MSKQNIQKRKSNVSSLKEQLTAGETKLQDGVFIFNGPLTISEFSLKISKNPTEILTYYFKKGIIKTINTTLSEDEIAELCFEFNLDFKKEENVDAQNLFNKLEIVEDPNDLASRPPIVTIMGHVDHGKTTLIDRIRHSNVTETEFGGITQHTGAYQVEYQGRKITFLDTPGHEAFTEMRSRGAKITDIVVIVVAADDGVKPQTIEAIDHAKAANVPIIVFVNKMDKQGADIERIKSELADNNVLCEEWGGNYQFVYGSALKNSNINELLEAIFLQADLLDLKANINRDPIGTIIESRIDKGRGVVSTVIVESGTLLPRDFIVAGGQYGKIRSLTSIDGKPIQKALPGTPCIITGLSKNPQSGDKFVAISDEKFAKKLASEKSYLDKCKELNEKNAFSIQDGIKTVNVIVKTDVQGTAEAIKQTLQKIRNDEIAVNVIRATSGEVTKADILLAETSNARIYLFNNTINNEIKKAAELKNIKIFKHNIIYKMVEELETLITTLKEPVYEEKLIGSATVLQLFFYSKIGQIAGCMMQEGIVKEFSKVEVYRRNKLIFKGQIISLKRGKNDVKSVEKGFDFGTHVKDFDKIEVDDELKFYEDVLVNEHN" }, "paralog": false, "identity": 0.9955156950672646, "score": 860.0, "evalue": 1.8e-316, "description": "Internal stop codon at 664080. Nonsense mutation around 664080.", "psc": { "uniref90_id": "UniRef90_D1J8W4", "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_Q98R05", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W4", "UniRef:UniRef50_Q98R05", "COG:COG0532", "COG:J", "KEGG:K02519", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R05" ], "product": "Translation initiation factor IF-2" } }, "id": "AHCKFKLKGB_1143", "locus": "GCA013367975_01142" }, { "type": "cds", "sequence": "contig_1", "start": 665143, "stop": 665376, "strand": "+", "frame": 1, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0006364", "SO:0001217", "UniRef:UniRef50_A0A448ZXY5", "UniRef:UniRef90_D1J8W5" ], "nt": "ATGAACACAATTAGTCACGAAAGAAAAACACAATTACTATTAGAATTAATTGGAAATAGTTTTTATGAATTAAAAGATTTTGATGTAACAAACATTTCAATAAATGATGTTATATTATCAAATGATGGTTCTCATGCCAAGATCTATGTAACTTTATTTAAAGATCAAAAAAGATACTTAGAAAAATTAACCACAATGACACCATTTATTCGTTCGGTTGTGGCAAAGAATTGA", "aa": "MNTISHERKTQLLLELIGNSFYELKDFDVTNISINDVILSNDGSHAKIYVTLFKDQKRYLEKLTTMTPFIRSVVAKN", "aa_hexdigest": "3fcfd61932e74de4b902676108094175", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "rbfA" ], "truncated": "3-prime", "pseudogene": { "start": 665143, "stop": 665484, "observations": { "mutation": [ 665374 ], "stop": [ 665374 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W5", "query_cov": 1.0, "subject_cov": 0.6814159292035398, "identity": 0.987, "score": 152.0, "evalue": 1.3e-46, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MNTINHERKTQLLLELIGNSFYELKDFDVTNISINDVILSNDGSHAKIYVTLFKDQKRYLEKLTTMTPFIRSVVAKNWKYKVLPELIFEIDTVEEHAAKIDKILNKIEEEKNN" }, "paralog": false, "identity": 0.9823008849557522, "score": 218.0, "evalue": 4.34e-75, "description": "Internal stop codon at 665374. Nonsense mutation around 665374.", "psc": { "uniref90_id": "UniRef90_D1J8W5", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_A0A448ZXY5", "cog_id": "COG0858", "cog_category": "J", "go_ids": [ "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W5", "UniRef:UniRef50_A0A448ZXY5", "COG:COG0858", "COG:J", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXY5" ], "product": "Ribosome-binding factor A" } }, "id": "AHCKFKLKGB_1144", "locus": "GCA013367975_01143" }, { "type": "cds", "sequence": "contig_1", "start": 665505, "stop": 665777, "strand": "+", "frame": 3, "gene": null, "product": "Glucose-inhibited division protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1T4L5B9" ], "nt": "ATGACCAATAATTTTGACGCTATTGTAATTGGCGGTGGTCATGCTGGAATAGAAGCAACTTATGCTTTGGCAAAAAGAAATTTTAAAGTCGCATTGGTAACTTTAAACATTAATAGACTTGCTATGCTTCCTTGCAACCCCTCAATTGGCGGTTCTGCAAAGGGAATAATAACACGTGAAATTGATGCCCTTGGCGGAGTTCAAGGGTTTTTTGCTGATAATGCAATGATTCAAATTAAGATGTTAAATACATCTAAAGGCCCAGCAGTTTGA", "aa": "MTNNFDAIVIGGGHAGIEATYALAKRNFKVALVTLNINRLAMLPCNPSIGGSAKGIITREIDALGGVQGFFADNAMIQIKMLNTSKGPAV", "aa_hexdigest": "1a1f7e54074d6f0611af994f07d089c1", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0021AE77E7", "query_cov": 0.9555555555555556, "subject_cov": 0.8113207547169812, "identity": 0.733, "score": 130.0, "evalue": 8.79e-38, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A1T4L5B9" }, "pscc": { "uniref50_id": "UniRef50_A0A1T4L5B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1T4L5B9" ], "product": "Glucose-inhibited division protein A" }, "genes": [], "id": "AHCKFKLKGB_1145", "locus": "GCA013367975_01144" }, { "type": "cds", "sequence": "contig_1", "start": 665856, "stop": 667184, "strand": "+", "frame": 3, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "COG:COG0445", "COG:J", "GO:0002098", "GO:0008033", "KEGG:K03495", "SO:0001217", "UniRef:UniRef50_Q98QV8", "UniRef:UniRef90_D1J8W6" ], "nt": "TTGATTCAAGATGAAGCAAGCGATTTAATTGTTGAAGATGGAACTTGCATTGGAATTGAAACACTTGAACACGGTAAACTATATTCAAAAGTTGTTATTATGACAACCGGTGTTTACATGAATTCAAGAATTTTGCGCGGTGAAAATATAAAATATGATGGACCTGATGGAGAAAAGACAAGTTCAACACTTTCAAAAAATTTAAAAAAATACGGTTTTGAAATAATAAGATTAAAAACTGGAACACCTTGCAGAATTTATACAGATTCAATAGATTTTTCAAAAGTTGAAAAAGAAGTTTTAGAAAAAAACGAACTTTGTTTTTCAAGCCGTAGTAATAAAAAACTAGATGAACAAACTTGCTGTTATTTAACACACACAACCTTAAAGACAAAAGAAATAATCTTAAAAAACTTAAAACGTAGTTCATTATATTCTGGAATCATAATTGGAATTGGTCCACGTTATTGTCCAAGTGTTGAAGATAAAATTGTTAGATTTCAAGAAAAAGATACACATCATATTTTCTTTGAACCAGAAACAGCACGTGGTGACATCATGTACATTAATGGGTTGTCTACATCAATGCCTGAAGATGTTCAAGATGAAATGATTGCATCTATTCCAGGTCTTGAACATGCAAGAGTTCAAAAATATGGTTATGCAATTGAATATGATGCAATTAACCCTTTGAATTTATACAAATCTCTTGAAAGCAAAATTTTAAAAAATTTCTTTTCAGCAGGTCAACCAAATGGAACGAGTGGTTATGAAGAAGCAGCAGCTCAAGGATTAATAGCAGGAATTAACGCTGCTAATAAACTTGATAATTTAGAACCATTAATCATAAAAAGAAATGACGGTTATATTGGCGTTTTAATTGATGACATTGTAACTAAAGGCACAAACGAACCTTATAGAATGCTAACAAGTAGGGCAGAATTTAGACTTCTTTTGAGAAATGATAACGTTGATGAACGTTTGTATAAATATGCATATGACAACAAAATGATAAGCAAAGAAGAACATGATAGGATTGAAGAAAAATATAAGATTATTGCCGAAACAGTAGAAAGATTAAAGAATGAATATGTTTCTTCTAAAAGTGATGTTGGTCAAAAGTACAATGTAACAAATGGCATGTCATATTTAAAATTACTTTCAAATCCCGAAGTAGATCCAGTTGATATTTTAGGCTTAAATTTCCCATATATTGATGAAATAACTATTCAAGTTCGTTTGTATGGATATTTAAAGAAACAAGAATCAGCAGCGGATAAAATGCTTAGATTGGAACAATTAAAATTACCAAAAGACATTGATTATTGA", "aa": "MIQDEASDLIVEDGTCIGIETLEHGKLYSKVVIMTTGVYMNSRILRGENIKYDGPDGEKTSSTLSKNLKKYGFEIIRLKTGTPCRIYTDSIDFSKVEKEVLEKNELCFSSRSNKKLDEQTCCYLTHTTLKTKEIILKNLKRSSLYSGIIIGIGPRYCPSVEDKIVRFQEKDTHHIFFEPETARGDIMYINGLSTSMPEDVQDEMIASIPGLEHARVQKYGYAIEYDAINPLNLYKSLESKILKNFFSAGQPNGTSGYEEAAAQGLIAGINAANKLDNLEPLIIKRNDGYIGVLIDDIVTKGTNEPYRMLTSRAEFRLLLRNDNVDERLYKYAYDNKMISKEEHDRIEEKYKIIAETVERLKNEYVSSKSDVGQKYNVTNGMSYLKLLSNPEVDPVDILGLNFPYIDEITIQVRLYGYLKKQESAADKMLRLEQLKLPKDIDY", "aa_hexdigest": "8e5f8c26fea518b55c70e1176b26b482", "start_type": "TTG", "rbs_motif": null, "genes": [ "mnmG" ], "truncated": "both", "pseudogene": { "start": 665556, "stop": 667325, "observations": { "mutation": [ 665775, 667182 ], "stop": [ 665775, 667182 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W6", "query_cov": 1.0, "subject_cov": 0.7293729372937293, "identity": 0.993, "score": 865.0, "evalue": 2.45e-314, "gene_start": 1, "gene_end": 442, "reference_start": 118, "reference_end": 559, "reference_sequence": "MTNNFDAIVIGGGHAGIEATYALAKRNFKVALVTLNINRLAMLPCNPSIGGSAKGIITREIDALGGVQGFFADNAMIQIKMLNTSKGPAVWSLRAQIDKEKYSEIILKDIEKQKNITLIQDEASDLIVENGTCIGIETLEHGKLYSKVVIMTTGVYMNSRILRGENIKYDGPDGEKTSSTLSKNLKKYGFEIIRLKTGTPCRIYTDSIDFSKVEKEVLEKNELCFSSRSNKKLDEQTCCYLTHTTLKTKEIILKNLKRSSLYSGIIIGIGPRYCPSVEDKIVRFQEKDTHHIFFEPETARGDIMYINGLSTSMPEDVQDEMIASIPGLEHARVQKYGYAIEYDAINPLNLYKSLESKILKNFFSAGQPNGTSGYEEAAAQGLIAGINAANKLDNLEPLIIKRNDGYIGVLIDDIVTKGTNEPYRMLTSRAEFRLLLRNDNVDERLYKYAYDNKMISKEEHDRIEEKYKIIAETVERLKNEYVSSKSDVGQKYNVTNGMSYLKLLSNPEVDPVDILGSNFPYIDEITIQVRLYGYLKKQESAADKMLRLEQLKLPKDIDYWSVDNLATEARQKLDKIRPETIGQAARISGINPADIQMLIFYLNNKR" }, "paralog": false, "identity": 0.9898132427843803, "score": 1145.0, "evalue": 0.0, "description": "Internal stop codon at 665775, 667182. Nonsense mutation around 665775, 667182.", "psc": { "uniref90_id": "UniRef90_D1J8W6", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_Q98QV8", "cog_id": "COG0445", "cog_category": "J", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W6", "UniRef:UniRef50_Q98QV8", "COG:COG0445", "COG:J", "KEGG:K03495", "GO:0002098", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV8" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" } }, "id": "AHCKFKLKGB_1146", "locus": "GCA013367975_01145" }, { "type": "cds", "sequence": "contig_1", "start": 667328, "stop": 667759, "strand": "+", "frame": 2, "gene": "rlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "db_xrefs": [ "COG:COG1576", "COG:J", "EC:2.1.1.177", "KEGG:K00783", "RefSeq:WP_175933616.1", "SO:0001217", "UniParc:UPI001588B87F", "UniRef:UniRef100_UPI001588B87F", "UniRef:UniRef50_Q98QV7", "UniRef:UniRef90_D1J8W7" ], "nt": "ATGAAATTAAATATTATAGCAGTAGGTTCTCTAAGCAAGGAATATAAAACTATCTATGATCAATATCTTAAAAAAATTAGTTTTTTTTGTTCAATAAATTTAATTGAAATAAAAGAACAAGTTAATAAAAATATTGATTTAAAAATAGCTGAAGAAACAAAATTAATCCTAGAAAAAATTCCTAAAAACTCCAAAGTTTATTATTTATCATTAAGAGGCAAGAAACTTGATAGTGGCGAATTTTCGGATTTACTTATTGAAGATAATATTACTTTTGTAATAGGCGGTAGCAATGGAGTTGATGAAACATATTTTAAAAATAAAATATGTTTTTCAGATTTGACATTTCCTCATCAATTATTTAGAATAATGTTAATTGAACAAATTTATCGAGGATTTAGTATAAAAAACAACATAAAATATCACAAATAA", "aa": "MKLNIIAVGSLSKEYKTIYDQYLKKISFFCSINLIEIKEQVNKNIDLKIAEETKLILEKIPKNSKVYYLSLRGKKLDSGEFSDLLIEDNITFVIGGSNGVDETYFKNKICFSDLTFPHQLFRIMLIEQIYRGFSIKNNIKYHK", "aa_hexdigest": "3cef87e0b0c87de57cede79a6b51f568", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001588B87F", "ncbi_nrp_id": "WP_175933616.1", "uniref100_id": "UniRef100_UPI001588B87F", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588B87F", "RefSeq:WP_175933616.1", "UniRef:UniRef100_UPI001588B87F" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588B87F", "uniref90_id": "UniRef90_D1J8W7" }, "psc": { "uniref90_id": "UniRef90_D1J8W7", "gene": "RlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "ec_ids": [ "2.1.1.177" ], "uniref50_id": "UniRef50_Q98QV7", "cog_id": "COG1576", "cog_category": "J", "kegg_orthology_id": "K00783" }, "pscc": { "uniref50_id": "UniRef50_Q98QV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV7" ], "product": "Ribosomal RNA large subunit methyltransferase H" }, "genes": [ "rlmH" ], "id": "AHCKFKLKGB_1147", "locus": "GCA013367975_01146" }, { "type": "cds", "sequence": "contig_1", "start": 668860, "stop": 669249, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGAATAAAATAAGTGCAGAAAAATTAGCAATTTTCAAAAAAATTGAAGAAAGAATTAAAGCACACAAAAATATAGTTATATATCATCATATTAGACCTGACGGAGATTGCTTGGGTTCTCAATTTGGAATGAAGAATTTAATTAAGGTAAATTTCCCTGATAAAACAGTGTATACAATTGGCGATTCCAAAGGTATTTATTCATTTTTAGAATTTAAAATGGATAAATTACCACTAGAAAAGTTAGATGATTCATTAGCAATTATTGTTGATGCTAACTTTAAAGAACGTTTAGAATGTAGAGAATATTTAGACAATAATTGTTTCGATGAAGTAATAAGAATCGATCACCATCCAAATGATGATGATTTGGATGCTTCATTAAGATGA", "aa": "MNKISAEKLAIFKKIEERIKAHKNIVIYHHIRPDGDCLGSQFGMKNLIKVNFPDKTVYTIGDSKGIYSFLEFKMDKLPLEKLDDSLAIIVDANFKERLECREYLDNNCFDEVIRIDHHPNDDDLDASLR", "aa_hexdigest": "0c2a38fe4e5df54da82c9499dd708430", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 100, "aa_cov": 0.751937984496124, "hmm_cov": 0.76, "evalue": 1.2987153796674269e-12, "score": 43.433292388916016, "start": 24, "stop": 120 } ], "seq_stats": { "molecular_weight": 15013.060199999985, "isoelectric_point": 5.709670066833497 }, "id": "AHCKFKLKGB_1148", "locus": "GCA013367975_01147" }, { "type": "cds", "sequence": "contig_1", "start": 669478, "stop": 669645, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATATTAACATAAACACATACATATCTCAAAACATGAAAATTGCAAACGACGTCGTTTCATTTTACTTCCCATTGTCTGAACAAATTAAATTAGGAATTAAAGATCCATTAAAAGCAAATAGACCTTTTGTTCTTGGCTCAATTGATAACACAAAGGCATGA", "aa": "MFDININTYISQNMKIANDVVSFYFPLSEQIKLGIKDPLKANRPFVLGSIDNTKA", "aa_hexdigest": "b8c4179f224329642b37b200e9fa8659", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6234.1592, "isoelectric_point": 8.063463783264158 }, "id": "AHCKFKLKGB_1149", "locus": "GCA013367975_01148" }, { "type": "cds", "sequence": "contig_1", "start": 670256, "stop": 670798, "strand": "-", "frame": 3, "gene": null, "product": "Phage abortive infection protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X7R6", "UniRef:UniRef90_D1J8X0" ], "nt": "ATGACATCAAAAGAAAAGAAAATGAAAATAAAAAATGTTATATTAAACATTATTGGTTTTCTAACCGTTGCTCTCTTTCTCGTTATTGCTATCTTGCTATTTTTAGCAGCAAATGGCATTATGGGAACTATATCTAAAAAATCAAGTATCGTTTGTTATGTTTTTGGTGCAATCTTCTTAGCTATATTCATTTTAATTGTAATTAAAATGATATTAATATTAAAAAAAGAAAATGTATATATAAAAAATGCAATCGATACAGATAAGCTCTTTGCAAATGCTTCTCTAAGCTCAGAAGAAAATGAAATTCATAAGCAATTTATTGAAAAATTTAAACAATATCAACAAAGTAGAAACATATACTTCGGATATTTATTTACTAAAGCATTAAGCTCATATAAAAGAGATAATATTGATATCTCAGACCACGAAATAAATTCATTAATTGAAAAAATGATTATTGACTGCCATAATGAATTTGGAATCTTTGATGTCTATTTAGCAATTGATTTAGCAAATTCATTAAATAAAAAGCTTGTTTGA", "aa": "MTSKEKKMKIKNVILNIIGFLTVALFLVIAILLFLAANGIMGTISKKSSIVCYVFGAIFLAIFILIVIKMILILKKENVYIKNAIDTDKLFANASLSSEENEIHKQFIEKFKQYQQSRNIYFGYLFTKALSSYKRDNIDISDHEINSLIEKMIIDCHNEFGIFDVYLAIDLANSLNKKLV", "aa_hexdigest": "1441e8f47cc5cfbdba23adc5f7ca56e9", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J8X0", "query_cov": 1.0, "subject_cov": 0.8450704225352113, "identity": 0.983, "score": 326.0, "evalue": 4.58e-112, "valid": true, "uniref50_id": "UniRef50_A0A501X7R6" }, "pscc": { "uniref50_id": "UniRef50_A0A501X7R6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X7R6" ], "product": "Phage abortive infection protein" }, "genes": [], "id": "AHCKFKLKGB_1150", "locus": "GCA013367975_01149" }, { "type": "cds", "sequence": "contig_1", "start": 670907, "stop": 671437, "strand": "-", "frame": 3, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:6.3.4.21", "SO:0001217", "UniRef:UniRef50_D5E5M9", "UniRef:UniRef90_D1J8X1" ], "nt": "ATGGCAGATAGGGCAGATCATTATATAAATCAGCCAAACGACTCATATGCAATAAGAATAGGTGGTATAGAAAATCACTCGACTTTTCTAAGCTCAAATTTTGATCCAAAGCATACTTTTGGAAGCATGCCTCATGCATTAATTCAAATGTTTGACGGTGATATTGTAGCTGCTTGCAAAGCATATAAAAATGTTTTTCCAAATGATGATCTAGTTGCCCTAGTTGATTTTCACAACGATGTTATAAGCGATTCATTAAAATGTTTAAAGGCTTTTGGCAAAGACTTAAAAAGCGTAAGAATAGACACATCAAAAGCGCTTATTGATAAAATGTTCAAAGAAGGCGAAGCAGAATTTGGCGTTACACCCAATCAAGTAAAAAGGTTAAGAGAAGCATTAGACAATAATAACGGCAAGCATGTAAAAATAACTGTTTCAAGCGGATTTGACGCTCAAAGAATTGCTGAATTTGAAGCCGAAGAGACTCCTGTTGACACATACGGCATCGGTGCAGCACTGCTAAAAATTTGA", "aa": "MADRADHYINQPNDSYAIRIGGIENHSTFLSSNFDPKHTFGSMPHALIQMFDGDIVAACKAYKNVFPNDDLVALVDFHNDVISDSLKCLKAFGKDLKSVRIDTSKALIDKMFKEGEAEFGVTPNQVKRLREALDNNNGKHVKITVSSGFDAQRIAEFEAEETPVDTYGIGAALLKI", "aa_hexdigest": "75d1c7c3d0104449e47eb3857299e092", "start_type": "ATG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "both", "pseudogene": { "start": 670799, "stop": 671737, "observations": { "mutation": [ 670909, 671476, 671536 ], "stop": [ 670909, 671476, 671536 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8X1", "query_cov": 1.0, "subject_cov": 0.5238095238095238, "identity": 1.0, "score": 355.0, "evalue": 1.03e-121, "gene_start": 1, "gene_end": 176, "reference_start": 125, "reference_end": 300, "reference_sequence": "MKKINLKDYISIYFYKTQKISKKFKKDQNITLQFFQRHNDVKLCGINEAIDILKQNTDISKYTIRYMPEGSIIHNREVVLELEGPYYEFGIWEGIIDGILARQSSIATNAYWAIKAANGKKIVSMADRADHYINQPNDSYAIRIGGIENHSTFLSSNFDPKHTFGSMPHALIQMFDGDIVAACKAYKNVFPNDDLVALVDFHNDVISDSLKCLKAFGKDLKSVRIDTSKALIDKMFKEGEAEFGVTPNQVKRLREALDNNNGKHVKITVSSGFDAQRIAEFEAEETPVDTYGIGAALLKIWVNFSADATRLNGHNIAKAGRGYLKNPQLITYKEAK" }, "paralog": false, "identity": 0.9903846153846154, "score": 613.0, "evalue": 8.1e-226, "description": "Internal stop codon at 670909, 671476, 671536. Nonsense mutation around 670909, 671476, 671536.", "psc": { "uniref90_id": "UniRef90_D1J8X1", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "6.3.4.21" ], "uniref50_id": "UniRef50_D5E5M9", "cog_id": "COG1488", "cog_category": "H", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8X1", "EC:6.3.4.21", "UniRef:UniRef50_D5E5M9", "COG:COG1488", "COG:H" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5M9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5M9" ], "product": "nicotinate phosphoribosyltransferase" } }, "id": "AHCKFKLKGB_1151", "locus": "GCA013367975_01150" }, { "type": "cds", "sequence": "contig_1", "start": 671534, "stop": 671809, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02749.22" ], "nt": "ATGAAAAAAATCAATTTGAAAGATTACATTTCAATTTATTTTTACAAGACTCAAGAAATATCAAAGAAGTTTAAAAAAGATCAAAACATTACTCTACAATTCTTTCAAAGACATAATGATGTCAAATTATGTGGGATAAATGAAGCAATTGATATTTTAAAACAAAATACAGATATTTCTAAATACACAATAAGATACATGCCGGAAGGCAGTATAATCCATAATAGAGAAGTAGTTTTAGAACTTGAAGGCCCTTATTATGAATTTGGTATATGA", "aa": "MKKINLKDYISIYFYKTQEISKKFKKDQNITLQFFQRHNDVKLCGINEAIDILKQNTDISKYTIRYMPEGSIIHNREVVLELEGPYYEFGI", "aa_hexdigest": "789c61941e0c5c9b56ebd75eaadd2a25", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02749.22", "name": "QRPTase_N", "length": 79, "aa_cov": 0.8681318681318682, "hmm_cov": 0.8481012658227848, "evalue": 3.480414812440199e-11, "score": 38.56007766723633, "start": 12, "stop": 90 } ], "seq_stats": { "molecular_weight": 10912.484899999996, "isoelectric_point": 8.625950431823732 }, "id": "AHCKFKLKGB_1152", "locus": "GCA013367975_01151" }, { "type": "cds", "sequence": "contig_1", "start": 672134, "stop": 672391, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8X2", "UniRef:UniRef90_D1J8X2" ], "nt": "ATGGTAAAATATCCATTATCTATAATATTTATAATTCTTTGCATAGCTATGATAGCAGGAATGTATACAATAAATGTAATGGGTTATAAACTAGCAATCAAATTAAACAATGAATCTTTAAGAAAATGCTATTTGATTTCACTAATTCCATTATTGTTTTTTGTAGCATTGGTAGCAAGCTTTGTGTCTTCAATTAAGCTAAAAGAAATTTATATAGATAAAAAAAGCCAATTTCAAAATGTTGCTATAGCAGGATAG", "aa": "MVKYPLSIIFIILCIAMIAGMYTINVMGYKLAIKLNNESLRKCYLISLIPLLFFVALVASFVSSIKLKEIYIDKKSQFQNVAIAG", "aa_hexdigest": "581490f79f53323a0d0b60fb7875800c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 671966, "stop": 672391, "observations": { "mutation": [ 672047, 672104 ], "stop": [ 672047, 672104 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8X2", "query_cov": 1.0, "subject_cov": 0.6028368794326241, "identity": 1.0, "score": 155.0, "evalue": 3.73e-47, "gene_start": 1, "gene_end": 85, "reference_start": 57, "reference_end": 141, "reference_sequence": "MAKNQDKKMLSPEEKEIENIKKISKNGWHFSYWSLFFILGVIAILIWAVESESSSSMVKYPLSIIFIILCIAMIAGMYTINVMGYKLAIKLNNESLRKCYLISLIPLLFFVALVASFVSSIKLKEIYIDKKSQFQNVAIAG" }, "paralog": false, "identity": 0.9787234042553191, "score": 262.0, "evalue": 7.21e-92, "description": "Internal stop codon at 672047, 672104. Nonsense mutation around 672047, 672104.", "psc": { "uniref90_id": "UniRef90_D1J8X2", "uniref50_id": "UniRef50_D1J8X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8X2", "UniRef:UniRef50_D1J8X2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8X2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9565.66899999999, "isoelectric_point": 9.555197715759277 }, "id": "AHCKFKLKGB_1153", "locus": "GCA013367975_01152" }, { "type": "cds", "sequence": "contig_1", "start": 672563, "stop": 672763, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTACCTTACCTATAATACTTGTAATTGTTGGTTATATTTTAATTACATACATATTAACAACATCAATACTATTGATGAAAAAACCACAATTAATAACTAAACGCCACACTTTAATTTCGCAAGTTGCAATAGCGTTCATTCCAACATTAAGTGTTTATGCTATAACTTCATTAGATAATTATTTTATCCTATGA", "aa": "MKITLPIILVIVGYILITYILTTSILLMKKPQLITKRHTLISQVAIAFIPTLSVYAITSLDNYFIL", "aa_hexdigest": "022642b7f95753253b7474b93d30f264", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7463.145299999999, "isoelectric_point": 9.777226829528807 }, "id": "AHCKFKLKGB_1154", "locus": "GCA013367975_01153" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Metamycoplasma hominis] [strain=SS25] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TTAAGCTGCTTTTGCTGTAAGCTTTGGTTCAACTTTTTGTTTAAAGAATTTTGTTCTTTGGAAATAGTGAACAAATATATGTTGCAATATTAATATAAATGCTCCAACAATTCAATAAATTTGTAGTCCGGCACTAAAGAATGCTCCAAAGATTATAAATACTAATGAAATAATATTTGACATACGATTTTGTTTTTTCATTGCTGCTTTTTGATAAGCATCAACACGTAATGACTTCTTTTTCTTTCAATTTAGAATCTTTGGCATATATTGCGATAGTGCTTGCATACCGACAGATATTAAAATTATTGGTAGATATATGAATTCTTTGTTAGAAAATACCCGGTTTAGTGAGTTTGCTGATAATTGAATTGAATATCAGGTTGCTTGTTTTATTTCTGGAGAGGTATTTATAACTCTAAATACAACAATTAATATTGGCAATGCAATAAACTGAGGCCAAATCTGTGATCAAGGAGAAATTTTTTCTTTCTTATATAATTCACTAATTTCAGCTTGCTTACGTTGCATCATTTGTCTGTCGCCTTTGTAAGCAGCATATTTAGATTCAATTTTGGCTTTCTTTTGGTTGAATTCTTCCATTTTTGCTTGACTAAACAAAGACTTAAATGAAACACCAAATACTACTAAACGAACAATTATAATAACAACTACTAATGCTAATATAATACTTCATCCTGTTGTTCCAAGTCCTTTAATAATTGAGTCCATAAATAAGTATATAGGTTGAACAAATATACCATAAAATGGTCCTTGTGCTCAATATTCTTTGTATGTAGAAATAGGCTTTTGACTCAATATTGCGCTATTATTAAATATTGTGTTTGTTCAATATTCTGAATTGTAATTTGAAGCAGGACTTAGACTTGATTTTCTATATACAAAACTACCGTCATTTGTTTTAGAAGGTAATTTTACATAATTTACATTTGATAAGTATTGTTGGAAAACACTTTTAACAAAAGTAAATAGTTGAAGCAACATTCTTCCTTCATCTTCATTGGCTAAAAGAGTTTTATTTTTTGCTTTTTGTTCATAGCTTTTTATAAATTGATTTCATTTTTCAATTATTTGGTTAGCATTTTCACTACCATTTAATTTTAATGCTTCTTTAAAGATGTTAACCTTATCATCTTTAAAGGTTTTCAAACTTGCTCATTTTTGTAATGTATTTGAAACAAGAATTTGAAAAATATCTCTTAAAACAATTTCATCAACTGATGAACCACCAGTTAACTTTCCTTCTACTATATCATAGTTAAATTCTTTTATTTTTGTTCAATCTGTTGGTATAATGCCATCTTTATCTTTTGGCTTAGTGTCAAACGATAAAGAATTAGGAATTAGAATTTCACTGAATTTGGTTAATGGAGTGTAATTTTTGGTTTTGTAATTTCCTTTTTCGTCACCCAAATTAAAGTAGGCATAATTTCCATTTACATTGTAGATGTAGCCACTTTTATCAGATGTTCCTTTTTCTGTTATATTTGTTCAATCTTCATCATGAGAAGATAAAACTTTTGCTTTGTCGTTTTTTGGCATTTGCAATTGCAACCCAAATGAACGAGAACCATATGCGCCATAATTAGGATCTCTTTTCCTTAACTCATTAATAGCATTAGGTTCTTTTAAAGCAAGATATGTATTGGCGATAATATTTTTATCATTTATTTTGTTTGGTAAGGTAAAGAAATTACCAGTACTATCGTATCTATAAGTGACAATATTTGGTGAAATGTCACTCTTTTTAGAATATAGCTCTTGTCCAGAACCGACTTTATTACTTGATTTTACAGTAAATGATTGTACACAACCAACTAAACCGATAGCCAAAAATAATATAACTATGACAATTTTTATTCATTTTCAAACTTTTAACCAAATATTTTTCGGTGTTTTGTTTTCTGGTTGACGAACTCGAAAGTCTTTATAGAATTTAGAGACATCCTTATTTGCCATCAGCGATCCTTTCATAAAGTTTTTTTACTTCTTTAACTTTTTGATTCCAGTCAAGACTCAAGAAGCCTTTGCGAGCAATAATCACAGTTTCGTAATTTAAAGTGCTAAAATCAATGTTTCTTAGAATTGATCTGATTTGATTTTTGTAATGATTTCTCAAAACAGCATTTGCAAATTGCTTAGGGATTGAAATACCCACTTTAAAGAAATCGGATTTGTTAAAATAAATTATTAAATTTTTTGAAACCACTTGATTGTGAGAATCAAGAATTTTCTGAAATTCCCAATTTTTTTTAACAACGTTATTTTTATTTATCATAATTTAAAAATATGCAAGGTATTAGACTTATTTATTGTCAGATACAGTTAATGTATATCTACCTTTTGCACGTCTAGCTGCTAAAACTTTTCTACCATTTTTAGTTTGCATTCTAGCCATAAAACCATGAACTTTAATGTGTTTGCGTTTTTTTGGTTGGTATGTTCTTTTCATAACAATTCTCCTTTGCGTTTTTATATATAAAATAACTTAATTTATTTTACCAAAAATACGACAAAAAAAGCACATAAAATACCTAAGAAAAAAAATTTAATATTTTTTCTGTTTTTAATATACATTAAAAGAAACATAAAATTCAAAGTTAATAACAAAATAAACAAAGAAATTATTTAAATGTTTATTATGTTATTTTAGTATAAAAAACCTCTTATTTTAAAAACAAAAGGCCTAAAAAAAATGTTAATAATTTTGTTAATAATTTGTAAATTAGCATTTATAAGGGCTTATTAATTTATTAATTTTGAATTTTAAAATATAATAGAGGTATGAAAACTACAAATCCCTCTGAATTAGATTTAAAAGCGCATAATATGTCATTTCAAAAAGAATTAGTTAATACTGCAAGTGATCCCTTAATATATGATCAATTCTTGTCGTCATTAGAAATTGTAAAAGAAAATAATAAAATGATTTACATATTAGTTAAAGGACCTGAAGATGTTTTAACATATATTAAGAACAGTCATAATGAAAGTATTAAAAGAGCAATTAAAAACGTTTATGGTGAAGAATATAATTTGGTTTATATTAGAAATTTAGATGAAATTAAAAGCTTGCAAGATAGCAATAATGTTGAATCTCCAACTAATTCATTGGCTAATAATTCTAAAGAAAATTTTAGCAATATTAAGAAGAATTTAACATTTGATGATTATGCAATAGGAAAATTTAATAATATGGCTTTAAAGGCAGCAAAGGCTATTTGCAATAGTGAAAAGATATTGTTCTCTCCTTTATTTATTCATGCTTCAAGTGGGCTTGGTAAAACGCATCTATTACATGCTATTGGAAATGAATTATTAAAACACGGTAGAAGTGCATTATATATAAATCCTGATTCATTGACAAGAAGATTAGTTGAACAATTAAAATCAAAGAATCAAGAACAAATTAATAAAATTATTGATGAATTAATGTCTTATGATTGTCTAATGTTTGATGATGTTCAACAATATGGTAATCGTGATTCTACATTAAATGTCTTATTTAATATTATTGATAATATGATAATGAACGATAAGCAAATAATCTTTTGTGGTGATAAAAAGCCGGATGATTTAGGTGGATTTGAACAAAGATTTATAACTAGATTTAATGGCGGATTAACTGTTGAAATTTTAAAACCAGAGTTAAATGATGTAATTAATGTCTTGAAATTTAAATTGCAAGAAAATGGTATAAATCCGGAACTTTGAGAGGAGGAAAGCTTAAAATTTATTGCTAGAAACTTCTCTTCATCAATAAGAAATATTGAGGGTGCAATTAACAGAATAAAATTATTTCAAGATGGTGACGACGGCTTTTTTACATATGATATTAATACAATAAAATCAATATTTAACAGCATGACTCAAGTTGCTGAGAATATTACCCCCGATAAAATAATTGATGCTGTTTCTAAATACTATAAAATTGACAGAAAAAAAATATTGAGTAATGTAAGAACAAATGACGTGGTTAATGCCCGCAGAATAGCAACATATTTAGTAAATAAAAATTTTAATTATACATTGCAAGAAATAGGCAAGGTTGTAGGAAATCAATCACATTCAAACGTTATTGTTTCACTTAATTGAATTGAAAAAAATGCTAATAGTAATCCAACGCTTAAATTAGCATTGCAAAAAATAGAAAGCAATCTTAAAAAAATTTCGTAATAGGTAGGTATAGAAATGAAATTAAAAATTGATAAATTATTATTAGATAATGCAATTGAAAGAGTATCAAAATCTGTGGATGCTAATCCATTTTTACCAGTCATGAAAGGTATTTTAATTCAAGCAGAAGATAACAAAATAACATTAATTGGTTCAAATGGTGAAACAAGCATAAAGCATGAAATAGAAACATCGATGGATGCCCAAATTGAATTGCCCGGAATTGTATTAGTTGAACTTAGCTTATTTAAAAATATTATTAAAAAGTTAGATGGCGAAATAGTATTAGAAGTTGTAAATAATATTCTTAAAATAAGTACAAACAACGAAAATTATAATTTAAATATATATGATGTAAATGAATATATTGCAATTGATTTTGCATGTTATGGAGAACAAGTAAAAATTAACTGAAATGATTTTAAATCTTTAGTTCGTGATGTTATTTTTGCCGCTTCAACAAACGAAGTTAGTCTAATCTTGTGCTGTGTCAATATATCGGCAGCCAATAAAAAAATAAAGTTTATAACAACAGACAGATTTAGATATGCCCAAGCAGTTCAAGACATTTCTGAAGATGTAACATTCAATATTTCAATATTAGCTAAAAACTTAAGAGATATTACCAACTTTGATTACAATGGCGACGTTATTTTAAATATTTCTGATCAAAAGATTTCATTTAAATTAGACTCAACAACTATTGAATCAAAAGTTGTTGATCAATTGTATCAAGATGTTTCAAGAATTGTTCCACAAGAATTTGACACCGAACTTAAAATAACTAAAAAAGAACTAAATAACTTGTTAAATAAGGCATCAGTTATTATTAATGAAAATTATAATAAAATTAAGTTGCACATTGTTGATAATGTTTTAACCATTTCATCTGCTAGAGAAGAAATTGCTAATGCTGAAATTAAAACAAATAATTTTTCTTACAACGGTGAAGAATTGAAACTCGCATTAAATAGTAGATTCTTAAAGGATGCTATTTCTGTATATGAAGATAATTTGAATATTTTATTTACAAAAGATAAATTAAGAATAGTCATAAAATCAGATTCTAACCCAAATGTGCTACAATTGATTACGCCACAAAAAGGATTCTAATGGAAATAGAAATTACTGGAGAATTTATTAAATTAAGTCAATTTTTAAAGAAAATTGATGTGTGCCCAACGGGCGGAATGGCAAAATATTTTGTTCAGGTTCACAGAATATTGATTAACGGGAACCTTGCAAACGGAAGAAATGCTAAGATTTATCCCGGCGATACCGTTTGAATAGATGACAATGTTTATTTAATAAAAGAACAAAAATAAAAATATTTTTTATAAATATTATAAAATATTATAATTATTAAGCTTAACAATAACATAAAGGAGAAATCATGCCAGGAAGAGATCAATTAACAGGTCAAAAAGCTCTAAGTGGAAACAAAAGATCACACGCTTTAAACACCGCAAAAAGAACATTTGACTTAAATCTTCAAAAAGTTACTGTTTTAACTGAAACCGGAAGTAAAAAAACAGTTCGTGTAACTGCAAAAAATGCTAGAACACTAAAAAAATATGGTCTAGTTGCATAGTAGTTTTTTATAAAAATAATATGAAGTTGCTTAGGCAACTTTTTATTTTAAAAAAATTAAGCAGTGGGCTTAATTTTTATTGTTTATAAATTTAAATAAATTAATAGAAATCTCTTTTGTAATTTTTTCAGCTCTTATATTTAAATCTAGTTCATTGCTTTTTAAAAATTCAGAAATTTTTTGTTTTAAATTGCTTGGAAAATTATTTAATAGAGTTTTACGTTTGTATTGAAACATTTGTTTAATAAATTTTAAGATTTCAACTTGATTGTTTCAAATAAAATCATTATTTTTTATTAGATTTAGTTGAATAACTGCCGAATCAACTTTAGGAGCGGGATTAAATTCTTTTGCCTTAACAGTGAATAAATATTTAGGTTCATAGAGAAGTTGTATTGATGCTGCTAATTTACTATATTGAGAATTACCTGCTCCACAGCATATTCTATCTGCTACTTCTTTTTGAACCATTAATGTTATTTTATTAATGTTTTTAGCATTATCTAATAATTTAAAAATTATATCGCTTGTTATATTATATGGAATGTTTCCAATTACTTCAAAATCAGCATATTTTAATAAATCAGTTTGTAAGAAATCCTGGTTCAATATTTCAACATTGCTTGAATAATTTTTTTGTAATAAAAATTCGTATAATCTTTTATCAAATTCAATACATACAAGTCTATTTACAACATCCGCCATTTGATCAGTTATTGCGCCCATTCCCGGGCCAATTTCAATAATATTTTTGCCTGCAACGTTCGCAGCCTTTACAATCTCTTTTTGAATGTTTTTATTGCTTAAGAAATTTTGGCCTAGTGATTTCAATGCTTTAATCATAGATTTTAAAAACCTTTCTAACATTCTCTTTAATTTGTTTGACTAAAACATCAACATCAATTTGTTTTAAATTAGCAATATATTCATATGTGTATTGAACAAAAGGACTAATGTTGGGTTTTCCTCTCATTGGAGTTGGAGCTAAATAAGGTGTATCGGTCTCACAAAATATTCTATCTAAAGGAATATTTTTTACTGCTTCTTGTAAAGGCTTAGCATTTTTAAAAGTAACAACCCCGCTTATTGAATAATAAATATTTGAATATGTCAAGCATTTATTTAAAAAAGTAAGATCCCCACTAAATGAGTGAAATATAAAATTAATGTCTTTATATTGAGGCAAGGTTATTATATCTAAAGAATCATCTAAAGCATCTCTAATGTGCATAATTACTGGCAAATTATGTTCTTTTGCAAAATCTATTTGAGCAATAAATCCTTGTTTTTGAATTTCTTTGCTAGGCGAATCATCATAATGATAATCAAGGCCAACTTCTCCAATTGCAACAACATTTGGTTCATATATCTTGTTTAAAATTTCTCTATCTTGCAAACCATTTGATAATGTTGGATGTATTCCAATAACAGGATAAACAAAATCAACCTTTTTAGCAAGTTCTATTGCTTGCAACGAATCATCTTCGTTTGTTCCACATACAAAAAGTTTTTCCATATTCAGGTTATGTCATTCATTAACTATTTCAAGAGGATCTGAATAGTATTCGGTTGATGGGTGGGTGTGTAAATCAATAAATTTCATTATAATTCTCCTATTATATCTTTAAAAATATCAATTATTTTTAATTCCCAAATTGCTTTTGATTGCCTATATTCTAGGTTATTATTTGATTCCAAACAATCCGAAATTACCTTGAATGAATAAAATGGTTTGCCAAAAATAAATGATGTATGTGCCAATGAACAACATTCCATATCGCCTAATAAATTATTTGTATTTCAATCTAATATATTTGAATTTATTATGCTTTTATTAATAAAACTATCACCGGTTATAATATTGGCTTTTTTAAAATTGCTGCAAATTTTTAGATCTTGATTGCAATAAAATACATCTTGCAAATTAGGCAATTGCCCTATTTTATAATTAGGCAAGGCGGTTAAATTAACATCATAATATTTGCAGTTTTGTATGCAATAAATATCTCCAACACAGCCATTAAAAATTGATATAGCAGGGCCAATGTTAATTACTTTTAATATATTGAATTTAGTCAATAAATAACTTAACATTAAAGCAGAATTAGTTTTTCCAACTCCGCAGTGCGCTAACAAAATTTGTTTATTGTTTCAATTTAATTCATAAAGATTTTGAAAATTATTTTCAAAATCAATTTTTATTTGTTTTAAATACTTGCTACATTTTAGTCTTAATTGTTCAGTTTCTAGTGGTTCAGCAAAAATAAATAAATAAAATTTCTTTTCCATATATTTTTATTATTATATTAATAAATTAATAAAAAATACATATTTAAAATAAATTAGTTATTTAAGTCGTTTTTGATTAAAATCAACAAATTAACTCAATATGGTTTTAAAATTAAAATAATATGTGAATATTAGAAATAGTTATTTCTGTATTAATATGAATTGTTATTATTGCAGGAATTGCTTTTGCAATTCATAAAAAGTCATCTCGACACAATTATAGTAAACCAATAAATTCAAACAAAAGAGATTCATCAAAACAACAAAAAGGCAACGAAGGAGAATTTTCAGTAGCTCAACAATTAATATTTTTAGATGAAAGTAAATATAAGGTTTTAAACGACATTCTATTGCAAATTGATAATGATAAAACAGCTCAAATAGATCATATTGTTGTTTCAACTTACGGTATTTTTGTAATTGAAACAAAGAATTGAAATGGATTTATCACAGGCGGTAGGGATTCATACTTTTGATTTAAAAGAAATTCGGGAAATAATATTGATATAATGAAGAACCCAATAAAACAAAATGAATGACATATTGAAGCATTAAAGCAGATATTTTCAAAATACAATATAAATATAAATAAAGAATTATTTTATTCTCTTATTGTATTTGTCCAAGGCCAAAAGCCCAAAATTGATGATCGGAATAATTGCTCTGTAATACCTCTCAATGATTTAGTAAAAAAAATTCATTCATACAATAAAGAAATATTAGAAGAATCTCAAATTAAAGAACTTATAGAATTGATTCAACAAAAAAATGTTGAAGATAAACAAACAGGTTAGAACACAACGAACGTGCAAAAAAAGTTTTAGAAGTTCAACAAGATAAAATTAAAGAACATATTTGTCCACTATGCAATGGCGAATTTACATATAAAAATGGTTTACACGGCAAGTTTTATGGTTGCTCTAATTATCCAAAATGTCATTTTTCAATATATGATATTGATTAAATTAATGACTTTTATAAGTAGCCTGATTATTTTTTAAATAAAAAATTACAAAAAAATATTTTTTTAACAATTTATTTAAAAATGGCTTATAATAATAACGCTATTAAATAAGAGCCACTGCCCAGGTGGCGGAATGGTAGACGCTACGGACTTAAAATCCGTTGGCTGTAATGGCCGTGCTGGTTCAAGTCCAGTCCTGGGCACCATAAATGCGCCCTTAGCTCAGCAGGTAGAGCAAATGGCTTTTAACCATTGGGTCAGAGGTTCGAATCCTCTAGGGCGTACCATTTCAAGAAATTGATCAAGAGAGCATTGCTCTCTTTTTTATTATTTTTATTTATAAAAAATCAAGTTTATTTTTAAACCTGATTTTTAAATTATTCAACTATTGATTCTAAATATTGAAGTTTATTGTTTTGACTATTGAATTTTATTTTAGCTCCATAAGCAATAATAGTTTTTGGATAACAATGTCCGAAATTAACATTGTAAATTATTGGAATATCAAAAGGAACAACTTTTTTTCAAACTTCTTTATATTCGTTATAATAAACACGATTTTGAGGGCATCCGACTAATATTCCTTGAATATTTTTAAATAAGCCAACTTCTTTGAATTTTAGCAACATTTTTTTAAGCAATTGTGGACTCGGCTTTTCTTCACTTGTTTCAAGAAATAAAACCTTATCATATAGTTCATTAGGTTTCAATATTAAATTATATTTTTTAAATATTTCAGCTTCTTCTTTGTTTTTTTCTTCATCTAACCCGCTATAAATAGTTTCAATGCAACCACCTCATAAACATCCCTCAAAAGATTTCTTTTCATTTAAAAATTCATATTTTGTAATGTTTCTATGTTTCTCTCTTGGTATATTCAATGCTTTTTTTGAAAAATCATTTCTTCTTCATATCAATATGCACTTGGTTTAATTTCAAATTTAGTTTTATTATCGAATAATCATTTAAATGAGTTTTTAGTATAAGGCAACATTTCTTTGTCAAGTTCAGCAATATCGCATAAAATGTTGTGCCCATAAAATGTTTTTAGTCCTAATCTATAAAACATTAAATGGTTAACGGTTGAATCTGAATATCCAATAAAAATTTTAGGATTATTTAAGACTAAATTTTTAAAGTTTTCGTCTTCTAATAAAAGTGGAATAGTTTTGTATGTGTCAAATCCACCAATTGCACAAATTATTGCTCTTACATTGACATTGCTAAAAACTTCATTTAAATCCTCAACTTTTAACATAGGATTTTCTTTTAAAACTTTTATTCCCTTAAGTGCGTTTTTAGCAATCATAACGTTAAGTTGAAAATCAAAATTGAGTCTTTTTATTGCTAAATCTAATTGGTGACTAGCAAATTCTTCTCCAATAGTTCCTGCAGATAAGCTTACTATTGCAATGCCATTCTCCTTGCTTAATTTTATTGGTTTGATCATATAGAAATTATATTGAAAAGTTTATTAAATTAAAACAAAAAAATTAGACATGCAACTTTGCACAAGTCTAATTTTTAGGGCTAGATTTAATTATTTATTGTTTTGAAAATATTTGAATAATGATTCAATATCTGAAATAAATTTATGTTTATTGATAATTAATTCTTTTATGCCGTCAGGAATAGATATATTGACCTCTTTATCAAGTTCTGCATTATCACTTGCCCCTAAAAAATAAAAGTCTTGGTTTTCTTCATCAACATAACAAATTAAAAACGTGTAATTATTATTTTTTTCTACGTCATCAACAAATAAAGCATTATCAGGTTTTTTATTGTCTAAATATTTTTTATAGCTAGTAACTATGTTTTTAGTTTTTTGTTGATTATAATCGTGTTTTGCACTTTTAACTTCAACATATAAATAATTTGTTACTTCACCATTATTTATTTTAAAAATAAAATCAGGATATGAAGACTTAATTTCTCTTTCTTCATCAAAATATTCTAATTTTAAACCATGAAAAACAGGGTTTTTTGTATAAACTTTGACGTTTATTTCATTTTTATATTCACTAATTATTTTCTTTGCTTCGTTAATGAATAATGTTTCGGGATTTGAATCAAAATGATGAAGTCTATCGTTTAAATCTTTATATGCATAATTTTCTGTTTTGTTTAGTTTAACATTATTTTTTTCTATTTGATCAGGGTCAATAAATATTTCATTAGTTTCAGGCAATGTTTTATTGGCTAATATTTTATAAAGTTGATTTGTTGAATCTATTTTTTCAATATATTGAGCATATTGCAATTTAAATGTATTGAAATATTCTTTTATTAAGATATATCAAAAAATATTTCTTGTTATTATATTGGCTTTTGAATAAATAGAATCATATTTTTCATTTAACGCTTCAATAAGAACTTCGTTGAATATTTTTTTATGCATAAGAAGAAACTTGTTGATAAATAATTCTAATTCAATTGAATTATTTATTCATGAATAAACTCTAATTTTATCTTCAATGTTTCCTTGTTCTCCTATTAATTTCTTGTTGTTTTTAATATATTCTTTATGGTATTTTTTAAAATATTCATCAATCTTTTCTTTAACAATAGAATTTTTAGCTTCTCTAATAAAATCTGCTCCTATTATTGCTTGTTTGATTTTTACTTTATTTATTTGGCCATAAACAAATTTTTCATTTTCAAATTCTCGTTTTAAAATCAATGTTCTTCTTATATTTTCTTTGGTTTGAGCATTTGAATAAATATAGTATCTATTGCCTTCTCTAGAAATTTGATCTTTGTTATAACTTGGATTTGGGAATCTTTTAATTCTTCCTATTGTTTGAATAGACAAACTACTTGATGACACTTTTCTAAGTTGCACAAGCATGCAAGCCCTCGGAATATTTCAACCAGTTGAAGGACCAATTTTAAAAATTATTGCATCAACATTAGAGTCATTTTTTGAAATTTCTTTTAAGTTTATTTTATGATTTTCTTCCTTTATCAAAGAAGAAGATTCCACTTTATCATTTGAAAAGTATTTAACATAACTCAAATTACATTCATTTAATTTTTTAATTATGTTCTCAATGCTTTCTTTAAAATCATCTTTTTCTTTGGCTGTTGATTTTTCACTTGGTTCATTTTCGACTTGAATAAGCATTGCTGGTCTTATATTTTTTAAATCACTATTATTGTTTGCATATTGGATTTGAACTTCTTTAAATGTTTGACATGCTTTATTTAGTAATTCATTATCATCAATTAAATCTTGACTCGAGTCAGTTATACCCTTGTTATAAACTAATTCATCTTTTAATAATTTAATATCATCTTCAGCTAAATCCTTTTCATCAACATAGACAAGTTTATGTGTTCCTTTAGGAGTTGCTGTCATTTTTATTATTAAATTGGCTGCTTTTTGAATTTTATATTCAAACCTAACTTCTTCTTTTTTTAATTTAGATTCATCATGGATTGTATCAATATCTTCAAATTTAAAATTACTTCTACCTTTTATATTTCCACCATGATGCGCTTCATCTCTAATATAAACTAATGTATAGTTTTGATATTTAATTTGATCAAGAAAGCTTTCAAGCAAACCTTGTTCGGTAAATATTCTATTTTTACCAAATGATGAAACACCAAAGATTATTACTTTATTTTTTTGAGGAATTAAATTATAAGTTGAATCTTTATTTTTTTGAGAATTCAAGGAAGGAGATTCCTTTCTTTCTACTTCATACAAACCATTAATATATCTTTTATATTCATTTAGGTTTGATTCCATTTGATAAGGTAAATCTGCTGATGAAAGTGTGGCAATAACAAAGACTAATTTGTTTGGTTCACCATTGGCATTATTTTCTTTTATTATTTCATTTATAGTATTAGCCAACATAAAGGTTTTACCGCTACCAGTTGGAGCTTGAAAATCAACTATTTTAGGTTCACTAGATTGAATTGCAATTTTATATTCTTCAAGCAATTGTTTAACTGCTTTTTCTTGTACACTAGTTAGATTCATTGCTATCTTTTCCTTCAACTGGTTTTAAAGCAGTTAAATTTAGTAATATTTCTTGTTCGTTAATTTTATTTACATTCGATAAGCTAAAATCATTCAACATGTTTTTAAAATCTTGTTTAATTATTTCATTGTTGTTGGCAAGCACATCAGTGTCATAATATTTAATTTCAAAGACATCAAGGTTTTGATTATATGGTTCGTTTTTCTTTATTCAATCGAAGTCGGTTTCACCCTTACTTCCCTTGCCATTATTAATTCTATAAAGCCTTTCATAGCAAACATTTATGCCAATATTATTTTCATTGTTTGTAACTAATGTGAATGTTCTATTGCCACCATCTTCTTTATTTAAATCTAAAACTGCATGACCTGTTGTTCCTGAACCTGCAAAGAAATCTAATACACGAGCGTTGGGATTAGGGTGAATGTTAATTAAATACTTAATTAGTTCGATTGGTTTGGGAAAATCAAAAATATTTTTATCTGAAAAAATACTTTTCATATTTTCACCCCCACCTTGGCTATAAATGTTATCTATAATATTTTCATATTGTTGGCCAGCACTAATTTTTATAATCTTTTTTGTTCTTGGATCAAATTTTACAAATTGATATTGCTTTCTAAAGGCTACTCATTCATCATTTGAATTTTTCTTACATTCGATGAATCCTAATTTATTTCCCGCCTTATATGCATCATAGCTTCATGTATAACGGCCCGACTTTGGTTTATATTTATTTACGTGCAACATAAAAGTAGTTCCATCTGGTGCTTCAATTGGATAATCTAGAGATTTCAAATATTGAAAACTACCGGTTGAACTATTACGAAAAAGTAAGGTCATTTTATAATATCCACGTTCTTTAAAAAAGACGTCTTTATTTATATAACCTAATTTCTTTAATTTTTCATCGCTATGAACAATATAATTTAAATTTAAAACATTTATATTTTTTGAATAGCAAAGAATATATTCTGTATTTTTAACAATTCCAAAATTAAAAGTAGTGTTACCTCCGGGTCCCCTCTTTTTTATTCAAGAAAAACATGTTATAAAATTTTCTTCCCCAAAAATTTCATCCATTAATACTTTTAAATATGCTTGTTCATTGTCGTCAATTGAAACAAAAATAACACCATCTTCTTTTAAAAGTTGGCGAGCCATTTTTAATCTTTCGTTCATCATATTTAGTCAACCTGTACGACTAAATTTGTCTCTATAAATAAATTTAGATGCCTCAACATTATCTTTTTCAGAAAGATTGTTGCCATCATCTTTTGCACTATCGGTATTATAAGGAGGATCAATATATATTAAATCGTAATTATAACCCCCCCCCTATTTGTTCTTTTTGTCTTTCAATTGCAAGTAGGTTTTTTAATGCATCATAGTTTTCACCAATAATTAAAGTGTTTTTATTAGTATTCAATAAATCATTTGTAAATGATAATTTTTCTTGTTTTTTTAATATTGCAATTTGTTTTGTATCAACACTAGGTGCAACATCAAAAACAAAGCCTGTTTTAACTCTTTGAATCAAAAGTTGAAAAACATTGTCCAAGTTTTCTTTATTTGAATTGTTTGTGTTAACTAAAATTTCTTTTATTAATTTCTTTTGGTCTTGATTTAATAAAAAAGAATTCAAGTTATCTATATCATCTATATAATTTTTTAAATCCTTCATGAGTTGTTCTCCTTGATATACCTAAATTTAAGATTTATATAATAATTTTAAATTATTTGATTATTATTTCTATGATTTCCGGCTTATTTCTTTACTATTTTTTAGTTAAAAAATTAAATTTAATAATTTAATTAACATATTTAACATATTTTTTTAACCTTAAATTTAAATATATTTGTAAAAAAATAAAAAAATTTGAACTACATTTTAATAAAAGAGTATTATTAATAAAAAGAAACGCAAACATGCGCATATATTTCGATATATGGCATTTTTATTTTATTGCTAAAAAGAAAGGGGGATAATTATGAAAAACAAAGAAATAGATGCTAAACAAGCAAAACAAGAAAGAGCAGAAAAATTATTTGGCCAAACACCAATAAAAAAAGCAATTTGAATTGTTGCTATTCCAAGTTTGCTTGCTGCAATCATGGCTGGATTATATGGATTTATTGACCAAATTTTTATTTTGAATTTGGTTCCAAAAAATAGAAACATCTTTGGAAATAATGAAAGTGAAATTGTTCAATTTTTAAATCCTGCCTTGCATAGTGGTGGATTTTTTAGTGATTATAAGATAATGATTGAACAATATAATTTACATTCAATTACAAAATTAGCTGAAATTACTCCTAATAGTATTGTTTCAACTGCGTCAGCAGTATTTGGCCCATTAATATTATTTTCTAATGCAATAGTATTTTTAGTACCTGTTGGAGCTTCAATTTATTATACTAAATGTGTAAGTAAGCAACTAAAACGGGCTGGCCAAGATTTATGGGCGACGATGTTTTGGTTTACGGTTGCACTTTCTTTATTTGCTACGTTAGTATCTTTTGTGTTTATATGAAGTGGATTACTAAATGTATTAGCAGGTGAAACTCATATTGATTATAATATTGCTAGAATAGCCGGCATAGATCCTAATGCTTTGCAAGATTATTACAGTGCTGCATATTCATTAAGCATAAAATGAGCAAAGCAATTTCTATATATTTATGCATCAGGAACAATATTACAAGGATTAACTCTTTATCTATCTTATTTTATAAGAGCAGAAGGCTATAACAGTTATGTAATGATTTGTGCTATAGTTGCAAATGTAATAAATGTTGCTTGTGATGCGCTATTTATTATTTGCTTTAAAATGGGAGTTTTAGGTGGTGTTATGGCAACCATTGTTGGTTGAACATTTAACACAATTACTTATTTTGCTTATATTTCAATAAAGATTAAACAAAATAAATCTTGGTTATCTCTTTCACACCTATTCAAATTTAAATTTAAAAAGGAATTATTGGGACCAACATTCTTACTTGGCGCAAGTGGATTTTTAAGAATTTTTGGAATAAGTTTTTCATTTTTAATTATGAATATTCTAATTACAAAAACCTCATTTTCAATGCCTGATTACTTCCAATATTATTGAGCAAAAACCCAACCAATTATTATGTTATTCTTGACATCAATATTTGGCATAAATGATGGTGCTAGAAGCTTATTTACATATAATTATTCGCTAAGAAACATGGATAGATGTAAACAAGTATATAAATGAACTATATTGATAGCAGTTCTATATTCAGTTCTTGTTTATATATTTATTGCCTTAACATCTAATAATTTGTGAATATGAGTTTTAAACATAGACCCTGATAGAATTGCAGGAACTAGTGTATTTATTAAGGTTATGTCACTTAGAATAATTGCCGTTTCATTTGTTGTTGTTTCATTGTTAGCATTTCAAGGTTCAAATGATATTGCAAAGTCTATCTTTGCCTCAATATTTGAAAACTTTATTTGCTTTGTAATAATTGTTCCTATTGGATATGGAATTGCTTGAGGAATATTTAATCACGATTCTGCCAATAGAGTAATTTCAAATTGAATCATTATTGCTGTGTTTGTTTTCAACTGTGTATTTGCTTCATCAATATTGATGTTTTATTCATGATGATTTGTCTATAAGAAAATGCCTTATATTGATCAAGCAAAAGTTAGCTGAAGTAGAAGAATTGAACATAAGTTTTTTGAACACGCAGAAAAACAAGAAGAACTATACAATGCTCAAATGCAACAAGAAAAAGTTAATGAAAGTAATAATTTAAAGCAATAAAAACTAATAAGAATTTATATAAAAAAACTTGCAAATTTGCAAGTTTTTTTATTTCTTATCGTTAAACAATTGCTTTAGTTTTATTGTCTTAAATTTTGTTGTTGGGCTGGGTGTGAATTGTTTTTACTATTGTAAGTTTTTACTGCAAAACATAATAATGCAATTAAAACACCTAATTGTGCAAGATTAGCAATTACTGAAAGTCCATATATTGCTATAGCAGTTTTAACAGCACCTGATAAAAACATTGCTGTAGCAACAATTGCTAAGACGAATGAAATTATAAACAAGGCAACTAATGAAATTGATAATTTTCTTGTTGTTATTTCATTTTTACTTAAACCGCGAGATCCTGTTTTAAATCCATAAACAACTGTTAGAATAAAAAAGACGATGCTTGCTATTGCTAATAATAATCCAAATACTGGTACAAGCGAACCACAAATAATATAAATAACATCTATTCCTAATAAGATTGCTGAAAATATTATTAGAAGTTTTGCATTTTCTTTGGTTTTCAAGTTTTTTGAATTATTTTCCATTACTTATTTTCCTTTCGTTTTATATAAAGCATAATAAATTATATAAAATTCATAATTTTTAAATATTTTAAAAATAAAACATCAAGTGTTTCAAACAACTAAATTTGTGTTGCTGCTTGATGTTTTTAAATAATTTTGTAATTTTTAGCTTTTATTGTTGATTGTCGGAATTTCAATTTTGAGGCTTTTGAGAGTCGTCACCATAGTTGCTTGTTTCATTATTAACATTATTGTTATTTTTAACGCAATAGATCTTAACTGCAAAACATAATAATGCAATTAAAACACCTAATTCTGCAAGGGTCAAAATTAAACCAATAATATATAGTGCAGATGTTGATGACACTAACAAAAATGGATTATATTCATTTGGGCCTTCATAATTATATGCGCCAAGTGATAAAGATATTGCTATTATTATTGCAACAATAACTATTGCAATTGTTGCTGCAAACAATGCTATTAATGCAATGGATAATTTTCTTATTATCATTCGATTACTATTTTTTGGTTCACGTGATCCTGTTTTAAATCCATACACAACTGTTAAAATAAAAAACACAATGCTTGCTATTGCTAATAATAATCCAAATACTGGTACAAGCGAACCGCAAATAATATAAATAACATCTATTCCTAATAAGATTGATGAAAATATTATTAGAAGTTTTGCATTTTCTTTGGTTTTTAAGTTATTATTCACTTCATTCTCCTATTTTATAGCTTAGATATTTTTTAATTATATTAAAAATTAGTGTTTTAAAATTCATTTTTTTTAAAAGCATAAAAAAAGTTTGCAAATATTATTGCAAACTTCTTATTTATGAATTAATTATTTGTTTGTAATTCAATAACATTATTATTTGAAACTGTTAATAAGGCAACATCACCTTCAACCAAATTTCCTTTTATGATTTCCATTGCTAATTTGTTTTCGATGTTTTTTTGAATATATCTTTTAATTGGACGAGCGCCAAAAGCGGAATCGTAAGCGTTATCAGCAACGAATTTAACTATTTTATCGTCAAAATTAATTACTAAATTATGGTTATTAATAACTCTTTTCTTTAATTTTGATAATTCTAGTTTTACAATATCACAAACAACGGCAGGGCTTAATGGGTTGAATGGTACTATTTCATCGATTCTATTAATAAATTCTGGTCTAAAACTTCTTAATAATAATGCCCTTACCCCATCAATGGTTAAACTCTTATTTATAATTTCACTACTTCCAATGTTTGAAGTCATGATAATTATTGTGTTTTTAAAGTTTACTACCCTTCCTGTACTATCTGTTAATGTACCATTGTCCAATATTTGTAACAATATATTTAAAACATCTGAATTTGCTTTTTCAATTTCATCAAATAAAACTATTGAATAAGGATTTTGTCTAATTTTTTCAGTTAGTTGTCCCCCATTATCATAACCAATATAACCTGGAGGCGATCCAATTAATTTTGACACGCTGTGTTTTTCCATATATTCAGACATGTCTAATCTTACAATGTGATTTTCATCATCAAATAAAGCTTCTGCTAAACTTTTTGCAAGTTCAGTTTTACCAACACCAGTTGGCCCTAAAAATAGGAACGAACCAATTGGTCTATTTGGGTCATTGATGTTTGCTTTAGAACGCTTGATTGCATCAGAGATTAGTTCAATTGCTTGATCTTGGCCTTTCACTCTCTTTTTTAATGTTTCAGACAAATTCAAAATTTTTTGTTTTTGGTTTTCTAGTAATCTATTAATTGGAATATTTGTTCATTTTGAAACAATATCTGCAACTTCTTCAGCATCAACAACTTCCTTGATTAAACGATTTTTCCTTGATTGTATTTTTTGTTCCAATTCATCTCTAGTTTTGATTAATTCAGGAACTAAAACATACATAATTTTAGAAGCTTCAACATATTTTCCTTCACTTTGTAGTAAAGGCAATTTTCTATTCAATTCTTCAATGTTTTCTTTAATTGAAGAAAATTTTTGAATATCTTCTTTTTCTTGCTTCCAGCGTTTTTCAAGGTCAACTTTTTGCAATTTTAAAGTATTTAGTTCTTTATCTATTTCTAAAATTCTTTCTTTATTTTTAATGGCTGTTTGCTTTGTTTCACTATCATTTGTTAGAGCAGCTTTTTCAATTTCTAATCTAGCAATTTGATAATTAATTTTTTCTAGAGGTTCAGGCAAATAGTTCATTTCAGTTTTTATATTTGATGCTGCTTCATCAATTAAATCTATTGCTTTATCAGGCAAAAATCTATCTGCAATATATCTTGCTGATAAATTTGCTGCCGAAACTATAGCAGAGTCATCAATTTTAACTTCATGATATGCTTCAAATCTTTCTTTTATACCACGCAATATAGTTATGGTATCTTCAATTGAAGGTTCTGAAATTAAAATTTTTTGCATTCTTCTTTCAAGGGCAGGATCTTTTTCAATATATTTTCTATATTCATCCAATGTTGTTGCCCCAATTAGATGCAATTGACCTCTTGCCATCATAGGTTTCAATATATTTGCTGCATCCATATTGCTATCGCCAGTTCCTCCGGCGCCAATTATCATATGAATTTCATCAATGAAAAGAATGATTTCACCATTTGCTTCGTCAATCTTTTTCAATACTTTTTTTAGTCTTTCTTCAAATTGACCACGATATTGTGTTCCTGCAACCAATGCAGGTAAATCAATTTCAATTAATTGTTTGCCTTTTAGGTTTTCTGGAACTTGATTTTCAACGATTTTCCTTGCTAGGCCTTCAACAATTGCTGTTTTACCTACACCGGGTTCACCAACTAAAACGGGATTATTTTTAGTTTTTCTTGACAAAATTCTAACTAGTGATCTTATTTCTTCATCACGGTTTATAACAGGTTCTAGTTTGTTCTGCGTAGCTAACTCAGTTAAGTTTCGACCAAATTCCTTCAAGGGATCTTTTTCTTGTTCATAAGGTGTATAACTTGCTTCCATATATTGTCCTCCTTTTACAATAAAAAATTATACTACAAAAATTAGCACTGTGTTAAAAAGAGTGCTAATTTTTTAATTGATATGTTGCCCATAAAAAAATTTGCTATTAAAATAATTTTATTTTTTTATAAAAAAATATTTTAGTGAAACATTATGTTTTTTTATTCTATAATTAATCCGGTTATTTCGTAACCGATTGAGTTAAGATGAAAGACATTATAAATAAACGATTTTGATTTCTATTTTTATTTATAAATGTTGCTACGCTTTGCATTTTTTTAGGAATTGCTTATCTAAACTGAGCTCTACTAACAGGATATTTGGTAGGAGTAATCAGTTTTCTTATTTTTTTAAGTGGCTTACATTTAGTATTTAAAAAAATGAATGATTGAAAAGAAAACGCTAGTATTAAAAAAAACAAAAACTTCGCAGTTATTATTTTTTTAATTTTAAATTTTCTTGCTTTATTGATTATTGCCTTATTTGTAATCTTTAACTTACTTTATAAAAACAAACATTCAAATGCAAATGTTGCATTTGTACCTTTTAACGTAATCACAATGGCTATTCCATATACATTATTTAGTTTACAAATAATAGTTATGGAATTAATTAAAAAAATTACCACTAAAAGGAATTTAAAGAGAAGGGAGGAAAATGGATAGAATTTTACGTCTTCATTGATTTGGTGGTCCAGTTAATCCTGGCGAAGTAAGCAGTTTCAATGACAACCAATTATTTACTTTAATTATTTTAGTTGCTTTAATTTTTATTATTTCAATATTAATTTTTGTAGCAATAAAAAGACAAAAAGTTGATAAAGCACCTAATACAGTTGTATTAATGGTTGAAAGTATAATAAAAAACAGTGATGAATTTATTTCTGAAACCCATGGCAATAAATTTGACAAAATGAATCCTTATTTTATTTCATTATTTGCCTTCTTGATTTTAGGAAATATGCTTTCATTAGTTGGTTTAGCCCCAATTGGAACTAGTTTGTCAGCAATTTTTGCTGCCACTGTTATAAGTTGACTTGGAACACACATCATTGGATTCATATATAAAAAATTTAAGTATTTATTTAAAATTCTTAATCCCTTAGAAGCATCATCAAACTTTTCACCATTAATAAGTTTAACTTTCCGTATGTATGGAAACATACTTGGAGGAGTTGTATTGGTTGCACTAGTAAGTTTATTCTTAAATAATATTTGAACCAAAATTATTGGCGCTAACTTAGAATCACCTTCAGCTGAGTTAAACCCTATTGGGTTATTGATCTTGCCAGCATTTAATTTATATTTTGATATTTTTGATAGTTTCATTCAAGCATTTGTATTTATGATTTTAACAATTTCATATTGAACGCAATCTGCTGAAGAAGATATAAAAGAAAAACATAAAGACAAAATAAAAGAATGAAAACAAGAAATCTTAAAAAATAAACAAAATAAAAATAATATAGATAATAAAAATAACGAAACCTCAGTAGTAGAGGCAACTATTTAGGAAAGGAAAAAAAATGGATAAATTAGAAAATATTGTTAATGCATTCAATACAGCATCAGCAGGTGTTAATAGTAAAGAAGCAGGAGTGAACTACCCTCTAGCATATGGTTTAACAATGTTAGGAGCAGGTATTGCTGTTCTTGGCTCAGGTTTAGTTTCTATTGGTCAAGGTATAGCCGTTGCTAAAGCCTGTGAAGCTGTTGGAAGAAATCCAGAAGCATTTTCAAAAATTCGTACATTGTTAATTTTGGGTTTAGGTATTGTTGAAACCGGATCTATTTATTGTTTAGTTATTTCATTATTGCTAATCTTCGTTTAAAAATATGTTTCAAAATTTTGCAAATTTATTAAGCCAATCGCAAAATGCAATTGCAGCAGCTAAGCCATCTGCTTCTGCTAAAATTGCAGAAGAACTAAACAAAACTTTTAATGGTTTAACATTTAATTGACCATTCTTTGTTTTTTCATTAATAAGCTTAGGCCTAGTTGTTTTGATTATTACTTTATTGGTTTATAAACCCATAAAGAAATTAATTAAAAACCGCCAAGAACTTATTCAAAAAAATATTGATACTTCAATATTAGCAAAAGAAAATGCTTTAAAAGTTCAAGAAGAAAATGATAAAAAAATTATTGAAGCTAATAATCAAGCAGCTTTAATAATTAATCATGCTAAATCAGAAAGTGAAAGAATTGTCAACGCAAGCACTATTGCTGCTAAAAAACAATCTGAACTTTTATTAGATCAAGCACAAATTTTGATTAATAAAAAGCAATCTGAGCTAGAACAAGCGCAAAAGAAAATAATAGTAGAAAATGCTGTTGAATTAGCCAAGAAAATTATTGGCCGTGAAATTAGAAATGAAGATAACATTAAATTAATTAATGAAGTCTTAGAAGATTAATATGCAAGATTTTAATGAAATCATTTATAACTGAGCATATGCAATATATGATCTTACAAATGAAAACAAAAATTTGGCATTAGTTTCTAATGAAGCTGCTGAAATTACAAGACTTTTAAAGAAAAATAAAGATTATTTGTATGTTTTAAATTCTTATGATATTGACGAAAATGAAAAATATCGTCTAATTGATGAAGCTTTTTCAAGATACAATTTTTATTTAGTAAATACTCTTAAATTAGCAGCTAAGCAACATGTGATTAAATACATTATTGTAATATTAACAAAATTTGTTGAACTTTCAAATGAAAAAAGAAACATTAAATACGGGTTTGTTTATACAATATCGCCTTTATCTGAAAGAGAATTAAAAAGGTTAGAAGTAAAACTTTCAAACGAGTTAAAGGCTAATATTCATTTAACAAATGAAATTGATCCCAAATTAATAGCGGGAATCAAAATAAAAGTTGACGATTATTTAATCGATAATTCAATAGTCAACAAATTAAATGACTTTAAAAAAGTCGTAGAAAATAATTAAGGAGGGAGGAATATGTCATTAGAACCAAATGATTTGTCAGCAATCATTAAGGCGCAAATAAAACACTTTAATGAAGACATATCTAAACAAGAAATTGGCCGTGTAATAACAGTTGGTGATGGTATTGCCTTAGTAAGTGGCTTGAGCAATGTAGAATATGGCGAATTGGTAAAATTCGAATCCGGCGTTATTGGAATGGCCTTGAATCTTGAAGAAGATTTAGTTGGTATAGTTGTCATGGGTGATGACCGCAACATTGTTGAAAATAGTACAGTTTATCGTACTAAACAAGTTATATCAACACCTGTTGGTGACCATTTATCAGGCCGTGTGTTAGATGCACTTGCTAACCCAATTGATGGAAAAGGTCCAATTCAATATGAATATACTAGACCAATTTTTAAAATTGCACCTGGTGTTATGACAAGAAAAGAAGTTAATCAACCACTTGAAACTGGAATTTTGGCAATTGATGCTATGATTCCTATTGGTAAAGGTCAACGTGAATTGATTATTGGCGATAGACAAACAGGTAAGACCGCTATAGCCATTGATGCAATTTTAAATCAAAAAGGTCGCAATGTTTATTGTATTTATGTTGCTGTAGGTCAAAAGAATTCAACTATTGCACAAATAGTTGATCAATTAGCAAAACATGATGCATTAAAATATACAACAATTATTTCTGCATCAGCATCAGACAGTGCTCCTTTACAATATATTGCACCATATACAGGAATTACTATTGCCGAAGAATGAATGGCAAGAGGTAAGGATGTTTTAATTATATATGACGATTTGTCAAAGCATGCTGTTGCATATCGTACATTATCATTACTATTAAGAAGACCACCTGGACGTGAAGCTTATCCTGGAGACGTCTTTTATATTCACTCACAACTTCTAGAACGTGCAGCTAGAGTAAATTCTGATTATGGAGGCGGTTCAATTACTGCTTTGCCAATTATTGAAACTCAAGCTGAAGATATTGCAGCATATATTCCAACAAATGTTATTTCAATAACTGATGGTCAAATCTTTACTAAGGAAGCATTGTTTAACTCTGGCCAACGTCCTGCTATTGATATCGGTTATTCAGTTAGCCGTGTTGGATCAGCAGCACAAACAAAATTAACAAAAAAGGTTGTATCAAGTCTAAAATTGGAATTAGCACAATACAATGAAATGTTAGCATTTGCTCAATTTGGTAGTGATCTAGACCAAGCAACTAGAACTGTTTTGGATCACGGTGCTAAAGTGTATGAATTATTGAAACAACCTCAATATTCTCCATATTCTTCAATAGAACAAATAATTATTCTATTTTGCACAAAATATCGTTTAATAAATGTAATTCCAAAAGAACAAATATTAAAATATAAAACTGATATTTTAAATTACTTTAGAACTAATCCTAATGCTATTAATACATTAAGAGCCGTTTCTGAAGCAAATGATTGAACTAACGAATTAGTTGAAAAACTATATAATGAAATAATTAGCTTTAATGAATTATTTGTTAAAACTATTCCATTATATAATAAAGATTTATATCAACCAGTACCTGAATTACCATGGAAAGTTTGCAAAAAATAAAACATAGAATTAATTCGGTTAATTCAACTAAAAAAATTACCAAAGCAATGGAGCTAGTTGCAACTGCTAAGTTTGCTAAAATTAAAAATCAATTTGAAAATATCAATAATTATTATCAAAGTGTTGAAAGAGTTTTTTTAAACTTAATTAAAAATTCTGATCAAAATATTGATAAATTATTGAACAGTGCTTCTTGAAAATTCAAAAGCAAAAGAAATTTATATATTGTATTTGGCAGTGATTTAGGACTATGTGGTCCATACAATTCAGAAATGTATAAAAAAATTACTCAAACTGTTGGAAAAGAAGATATCCTAATAGTAGTTGGTAGCAAATTATTGAGTTTAATTCAAAAAGATAATAGCCATCATATTATTCAAATATTAACTCAAATTGGTGACAATCCAAGTTACGAAATCGCTCAAATATTGTCAAATAAGATTTATCATGTTTTAGAAATATCATGGCTAAAATCTATTAAACTTATTTATACTCAATACATTAATCCAATAAAATCTGAACCAATAGTAAAAGAAATTTTTCCAATTTCTATTGATACACTATCAAATAATGATAACAATTTAGCAGGGGAAGAAAGTTCAAAAAGTATTGATCCTTCTTGCTTTGAACCAAACCCTACTGTAATATTGCAAAATTCATTTTCAATATTCTTTGAAGCCTCTATATACAATGCCTTAGCAAATGCTAAGCTTGCAGAAATGAGTCAAAGAAGAACAGCAATGGAACAAGCAAGTGATAATGCACAAGATCTAATTAATGAATTAAATGTTCAATACAATAGTTCTCGTCAAGCAAAAATTACACAAGAAATTACTGAAATAATTAATGGATCAAATTAAAAAGAAAGGAGTAAAAATGGCAAATAAAAAAACTAATGAAAATCAAGAAATTCAAGAAGAACAAAACCCAGTTGATGAATCTAAAAATTCAGAAAATTATGGCGTTATTAGCCAAATTTTGGGATCTGTTATTGACGTTAGATTTGAAGAAGGACATACTCCAAAAATCTTAAATGCTTTAAAAATTATTGATGAAGACAAAAAATTTAGCAATGAACAAATTCTTGAAGTTGCTCAACACGTAGGAAATGATACAGTTAGAACAATAGCAATGAACTTGACTATTGGTTTAAAGCGTGGTATGAAGGTTCTTGATTTGGGTTCTCCAATAAAGGCGCCTGTTGGTAAAGAAGTTCTATCTAGAATGTTTAATGTTCTTGGTGAACCTATAGACCAAAAAGGTGGTGTATTCAAACACAAAATGCCTATCCATAGAGCAGCGCCAAGCTACGAAGAACAAAAATCAACACTTGAAATATTTGAAACAGGAATTAAAGTTATTGACTTACTTGTTCCATATATTAAAGGTGGAAAGATAGGTTTATTCGGAGGAGCCGGAGTTGGTAAAACTGTTTTAATTCAAGAATTAATTAACAATATTGCAAAACAACACGGCGGTCTTTCTGTGTTTGCAGGTGTTGGAGAACGTACAAGGGAAGGTAATGACCTTTACCACGAAATGCAAGCAAGTGGTGTTCTAGAAAAAACTGCTCTAGTATTCGGTCAAATGAACGAACCTCCTGGAGCCAGAATGAGAGCACCATTTACTGCTTTAACTATCGCTGAATACTTTAGAGATGTTATGCATCAAGATGTGCTATTGTTTATTGATAACATATTTAGATTTACACAAGCAGGTTCTGAAGTTTCTGCATTGTTAGGTCGTATGCCTTCTGCTGTTGGATATCAACCAACATTGGCTGTTGAAATGGGTCAATTACAAGAAAGAATTACCTCAACCAATAACGGATCAATTACCTCTGTTCAAGCTGTTTATGTTCCTGCCGATGACTTAACAGACCCCGCACCAGCAACAACATTTACACACCTTGATGCAAAAACTGTTTTGGACCGTGATATTGCTGCTTTAGGTATTTACCCTGCTATCGATCCTTTGGCTTCATCATCAAGAATGGTAGATCCAAATATAATTGGTCTTGAACACTATAACACTGCTAGAGCAGTTCAAAATATTCTTCAAAGATTTAAGGAATTACAAGATATTATTGCTATATTAGGTATGCATGAATTAAGTGAAGAAGATAAGAAAGTTGTTGCAAGAGCAAGAAGAATAAGAAACTTCTTATCTCAACCTTTCTTTGTTGCCGAAAAATTCTCTGGTATTAAAGGACAATACATAAAATTGTCAGATACTATTAGATCATTTAAAGAAATTTTAAGTGGAAAATATGATGAATTGCCAGAAGAAGCATTCCTTTATGTGGGTACCATTGACGATGTTATAGAAAAAGCTAAAAGACTTGGGGTTAATGTTGAAAAATAATGGAAACTCCTAAGATACATATTATTATTAGTACTCCATTAGGAATATACTTTGATAAAGAAGCAAGTATTTGTACATTTACTACTACTGAAGGGCAAATTGGAATTATGGCAGGCGCAACTGAGTTTATGGCAGCCTTGGTTCCTTCAAAAATAATGGTTAATTTTTTAAATTCACCTGATTCTAAAGTTTTTTATATTGATAAGGGAATTGTTCAATTTAAAAATAATGTCTTATCAATAATAGTTAATCAAATTGATACTAAAGAAATTGAATTGGATCATAAATTTAGCCCCGAAGATCAAAAGAAATATACAATTATTGAAGAAGTTACTTTGAAAAAGAGCATTTTGAAAAGTCAAACAAACAATAAATAATTAATTTTAAAAAAGTAGGAATTTAAAATCCTGCTTTTTTTATGCTAATTTTTAAACTAAATTTTTATAATGAAAAATTTATCAAATTAATAGTTTGATTAAAAAGAAAAATTTCTGATTTCAATTTTTAAATTTTAAAATATTTGGGCCATTTTTTATATGTTTAAAATTCTTAAAATTTGTAATTCTTTTTATAAATTTTAGAATTATAATTCCACAGTACAACAAAAAACAAATAAGGAAGGAAGATAATAAAATGAAAAAAACAGCAAAAGCTTTATTAATAATATGCACAACTATAATACCTAGTTTTGCTATGCCATTAATATCGGCGGGATGTAATAAAACAACAAACCATGAAACAAACAAAGAAAACACAATTATAGATAAACAGGCCCCTATTAAAAAACAAGAAAATGATTCTAATAATCCCAATAAGTTAGAATCTAATAGTGAATTAGCAGAAGCAGTTAAAAAAGTTAATTTTCAATATAAAGATATCGATAAAATTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAATAACCTTGATAAAAAATTTAAAATAGAAATTTTAAAATTAGAAGCAGACAAGAAGAATAATTTAATATCGATCTCGTATAATATTTCGTTTAATGACGGAAATAAAATCACTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAATAATGAAAGCTATTTAAATGGATTATTGAATGATAATGACACAATGTTTAATTTAACAACTAATCTCAAAAAAGGTAATGAAATAGATGCATATCATTTAGCTAATGGCCAAGGTTCTATTAGTTTGTTGGCATCTAATAAAAATGTTTATATTCCCAAAGATCTAATCTTAGAATATACTTGAAATGAACAAAACGAAACAAATAAAAAATTCAATGGTGAAGTAGAACTTGATGTTAGTGTTAAGACTAAGGACAATAAAACTATTACAAGAAAAAATGTAAAAATCGCTAATAAAATTGCTGAAGGCGTTTCAGATGATTATTTAACTAAATATAAAAAGTTAACTTTAAATTATAAAGATGCTGACAAGACTTCAATAGATTCTTTTAGCAAAGGTGATATTGAACAAACGTTAAAAAATAATTTTATTGTTAAATTTGAAAAGAGCAGTGAAGAAGAAACTAAAAAATTCTTAGAAGACAATGAAATTAAATATGAAGTTAAGAGCATAACTGAAATTTCTCTTGAAAAGAAAACAATCAATGTTAATCTTCAAATAACATTAAAAAATAAGAAACTTGTCAATGCAAATATTGAAGTTGCAGGATTTAAAGGCGTTGAAAAAGTAAGCGATGAACAAGTTAAAAAAATAGTAGATAAATTAACCTTTGGTGATGTTTATTTCCCTGAAAAAGTTAAAACCTTTAGTCAAAGTCGTTTAAACAAATTAAATTTATATATTAAAGATGAAAACGGAAAAGCTCAACTAGAAAATCCTTTAGATTTTGCAGAATATTTTACAAAAAATGGAATTAACAATTTTGGTAAAAACTTCATAGTTAAATATGATAAAGCCGAAAAGAAATTAATTACAACATTAAAATTGAATGAAAAAGAATACACAATTGAAACAAAATTAACGCATGAATCAAAACCAGATGAATGGGTCAACGAAGTTGGTAAGGAATTTTTAGGAAACCCAAATGAAATTTTGGCAAGTGATTTCTCGGCTACAAATTTAAAATTAAATAAGAATGTTGGCGATAGTCTTGACAGTTTAAATTTTATTAAATTATCTTCAATAAAAAGACCTTCAAAAAATTCAGAGGAATTAAAACTCAACGAAGAAGGCAAGGCAACGTTAATTTATGAATTTAGAAATATATATGATAATTCAATATATACAAAAGAAGTCGTATTTGAAAATCTAAAAAAAGTAGAAAAAGCTGATTATAAAAAACAACACGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTAAAAGTGGTTGGGCTACTGACGAAGAAATAAAGAAATTAGAAACTTTTTTGCCAGGCGAAAAAAAATCTAAAAAAAGTAAGAGAATAAACATTGAACATTATATAAATAATGGCAATATAGAAATGCTTGTTGGAAGAGTAATTGTTAAAAAAAATAAAATTACTTTCGGCGATGCATTTAAAAGACAAAAAGAATTTAAAGAAATAGTATTTGATGAAGAATTTGTTGAAGCAGTTAAGAAGTCGTATGATAATAAAAGCGAAAATAAAATATTATCTAATGTAATAACAGATGAAAATTCTAAACGTAAAAATCAAAAGAATTGAATAAGGCTTACAAAACATGAAGGAAAATGATGTATATTATTTGCATTCATTGAAGATACAAAGTCAACGCCATATTATGTTCAAATAGAAAAATAGTCTTATAAAATTTTATTGCAATAATAAATGAAATAAAAAAAATTAAAGAGTAGGGGATTGTACCCTACTCTTTTATATTGAAACAAAATAGTAAAAGCATAAAAAATAAAGTTAAAAGTTTTTATTTTTAACCAAGAAGTTTAAAATATTTGTTTTTTGTTAATTTTTAAGATGAATGCTCATTTTTAATCACCTTAGCAAATAAAATTATTTTAAAATTAAAAAAAGGAAATACAGAATATGAAGAAATCGAAAAAAATTTTGTTGACCATGGGAGGGATACTTCCCTTGGTTATTTCAGCGCCATTAGTTGCTGCTGGATGCAAGGATCCAAACAAACCAGAAGTTAAACCAGAGGATTCAAAGAAACCAGAAGGTGAAAAACCCGGAACAACTCCTGAAAAGAAGCCTGAAGTTAAGCCGGAGGCTGAAAAACCCGGAACAACTCCTGAAGTTAAACCAGGATCTAACAGTGATTTAAATGATTTAACAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAGATTTCAATGTTTGACAAATTAAACAAAGAAAACTTGTCAAACAACTTTGATAAAAAATTCAAAGTAGAAATTTTGAAATTAGAAGTAGATAAAAAAATTAATTCAATGTTAATTACATACAACATTTCGTTCAATGACGGAAGTAAAGACGCTAAATTAGAAAATCAAAAATATTCTTTGACAGGACTTCCAACAAACAATGAAAGCTATTTAAATGGATTATTAAACGATAAGGATACAGTATTCAATTTAGGTGATGCTTCTAATGATGATAGTTTAACAACAGTTGAACAGTTAGATGCATATTATTTAGTTGATGGTCAAGGCTCTATTAGTTTATTGGCATCTAATAAAAATGTTTATGTTCCCAAAGATCTAATCTTAGAATATACTTGAAATGAACAAAATGAAACAAATAAAAAATTCAATGGTGAAGTAGAACTTGATGTTAGTGTTAAGACCAAAGACAATAAAACTATTACAAGAAAAAATGTAAAAATTGCTAATAAAATTGCTGAAGGCGTTTCAGATGATTATTTAACTAAATATAAAAAGTTAACTTTAAATTATAAAGACGCTGACAAGACTTCAATAGATTCTTTTAATGAAGGCGATATTGAACAAACATTAAGAAATAATTTTATTCCCAAATTTGAAAATAGCAGTGAAGAAGAAACTAAAAAATTCTTAGAAGACAATGGAATTAAATATGAAGTTAAGAGCATAACTGAAATTTCTCTTGAAAAGAAAACAATCAATGTTAATCTTCAAATAACATTAAAAAATAAGAAACTTGCCAATGCAAATATTGAAGTTTCAGGATTTAAAGGCGTTGAAAAAGTAAGTGATGAACAAGTTAAAGAAATAGTAGATAAATTAACTTTTGGCGATGTTTATTTCCCTGAAAAAATAAAAGATTTTAATCAAGAATTTTTAAATGAACTACCTTTATATATTAAAGACAATGATGGAAAAATTAAACCCGAAAATACACTTGATTCTGAATATTTAATTAAAAACGGAATCACAGAATTTGATAAAAATGAATTTGGTCAACCTAAGTATGAAGTTAAATATGATGAAAATACAAAGAAATTGTCCGCAACATTAAAATTAAACGGCAAGGAATATAATGTTGAAACAACATTAACTCATGAATTAAAATTAGATGAATGGGTAGATGAAGTCGGAAAAGAAATTGATGGGGATTCAAATACCATTCCCGTAAATGAATTTTCTAATAAAAATTTAAAATTGAATAAAAAAATTAATGACTTCGTCAATGGTTTAGACTTTGTTCAATTAAATTCAATAAAAAGAGCAAAATCTTCTGAGGCAGAAAAAAAATTGAATTTAGAAGGTAAGGCAACCTTAATTTATGAATTTAAAAATATATATGATAATTCAACATATAGAAAAGAAGTCGTATTCGAAAATCTAAAAAAAGCAGAAAAAGTTGATTATAAAAAACAATATGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTACTAATAATAAATGAGCTTCGCCAGGAGAAATAAAAAAAATAAGTGCCTTTAAAAGCAATAAAGAAACCTATTCTTATAAAGATAATGAAGGTAAAGAACAAAGTGCAGCATTAACTGGTAGATTTAAAATTAAAGGAAAATCAGGTGATTTAAAATTGCATTTTGGAGATAATAACAGAACTAAAGAATCATTCAAAGATTTAAAATTTGACAATGATTTTCTTGAAGCAATAAAAAGTAAAACATTAGTTAATGTTGTTAAGGATTCTGGACATGGCGAATGAGTTAGATTTACACAACACAACGGCAAATGATGTATAGTATTTGCTTTTAAAGATGATCCAGAACTTACACCTTACTATGTTCAAATAGAACAATAATAAGTTCAAAAATAAAGTTCATTCTATTAAAAGAAAGGACTTTATTTTTTATTTATAAAATAAAATTTATATATTATAATAAATTATTACAAAAAGCAAAAGGTGGGTAAAATGGTTAAGAAAAAAGTATTATTTAAAATATTTGCTATGGGTACTATTCTAGCCGGAGGCGTTTTTAGTTTAAATTCATGTATTTTTAAATCAAATGATACAGAAAATAAAGATAATAGTTTTGAAAATAACACTAATAACGGTCAAAGCAACCAACATGATTTAGGCACCGAAGATAATAATTCAAGAAATTTGATAGTTCCTGAATCAAATAATAAGCCCGAAAATGATTCTATCCCTCAACCTGAATCAAAACCAAATATTACCCCAACGCCCGAACCAGCGCCTGAGCCAGCGCCTGAAATAAAGCCTGAACCAGCGCCTGAGCCAACTCCCAAACCAACGCCCGAACCAAAAGAAAATGATCATAAAGCCAATCATAATCAAACTCATTTTGTAAGGCCACCTTTTATTGCTAAATTTAAAATTAAAAATACTGATTTTTCAATAGCGACAATTTTTAACCATTTAGATAGTCCTGGTTCTAGAAAGAAAGAAAAATACATTGAACCAAAACCATCGATTTTATTGGCTGATAGTTCAAATAAACAGTCGGATTTGAATAAACAAGGAGCCCAAGAAGTAAGCGAATTTCTAGGCTTAATAGATGTTATTAATGAAAACAAAGATTTGGCCAATTTTGTTATTTATGGTGGCGATACTAATATAAAAAATGAAAATTTTTATTTAGCAAGAAAATATCCCGAAAATATTCAATTTACTTTAGATAGAAACTTAAAATTAAAGAAATATGACCAAAAATTTCTAACATCATTAGGAACAAGGGGTGGATATTCAGAACAATACGACAAAATGTTCTTTATTAATCAATTGCCCAATACATTTAAACCAAAAATAATTACCAATGGTCTTTTACCTTATAAGGTTGATATTTATAGAACGTTTAATGATGTTATTTCAAAAGCAGAACTTGAAAAATTAAAAGGATGAAAATCAAAAAAGAACGATAGATCTACAATTAGAAGTCAAATTTCAGACCATGCTCCAGTATTTACAGATGTCGTATTTTCAAATCCAATCGAAACGCTAAAAGTAAATGAAACATTAAGAAAAAACCAAACTTTATCTAAAGAACAAAACACTATAAGAATAGCTCATTGAAATATATTAAATTTTGGTAGTCCTAATCAAGATGCTAAAACAATGGCTATTGCTGCCACAATTTATAAGTCCGGTTTTGATATAATAGGCCTAACTGAAATTAATGACGGAGGAGGAAACTATGTGCAAAATATAGTTAATCTTTTAAATGAATATGCAGGAAAAGAAAGATATAAATTTGTATTACAAAATAAAAAGGATACAAATATAAGGCCTGAATATTTACAATCAAATAAGTTTGGAAAAGCACAACAAGAACAAGTTGCAGTGATTTATGATAGTCAAAATTTTGAATTTGTTTCAAGCCAAAGCTATTTAAAACCAATTAAATACTACAAAGATAACTAAAAAAACTTGGATGCTTCCAAGTTTTTTTAGGCTAATAATCACCTTTTCTAATTTTATTTAATCCACTTCATTTGTTAATTGAAGTTTTATTATTCATATTCATGAATTTTCTATAACCACCTTCTTTGATAGTCATTAGAATATATTCTTTTTTAGAAACTTCATTAGGTTTTCCATTTTCTTTAACTTTGAAGTTTACATTTTTTAATTTACTACTTTCAGATTTGTTATATCCACTTGTTGTAATTAAATAAGTAATTGATTCAGGACCATAATTTTCTTCATTATAGTATTCTCAAGAATGACTAAATCATCCTCGAGTTTCATTATAAAGATAGTTAGTAAATTCTCTTCTTCTTTCAGGGTTTTTAAAATAAGCTTGCATTCCTGTAAATTTACTTGATACTCCAGGAATTGCTTTAAGATTAGTTCCTTCTTGAAATTCTCAAAGTATTGCCCGTGTTCCTTGGTATAGGCTCAATGTTGGCGCATTATTTCATGTACCAGGTTCTCTATCTTTTTCCAATGTTTCTTTACCAAACATTTCAAATATAGCAAAATCTACTGCTACATTAAAACGATATACTGCTTTTCATGCTTCAGATTGTGCCCTAACTTTATATGTTGGTGAAGTTGCATCTTTATCATTATTGTATGCCTTTGCAAGCAATTTATAAATTGTATCAAGTAATTTTAAAACGTCTGCTGAATATTCAATATTATCAGGGTCATCTTTATCGCTAGCTTTTGAAGAAGATTTGAAAATGCTTCCCAACGCATTTGAAACCGAGCTATTTAAGGTATTCATGTTTGAACGTTTTGATTCGTCTGAATTATTGTTTAGATTTCTTAATGCATTATAGATTACAGAGTTATTAAAATTACTTTTTCCAAATACTAAATTGATTAATTTAACCAAAGCAGTTTCATTGATTGGTTTATCTTCACCTACTAATTCAGACAATACTAAAACATCATTAAAGCTAAATGCTGATTTAATTTTTTCAATTGCATCATTCAATCATTTAACAGGATTTAAAATATCAAATTGTTTAGCATAATTGCTTATCAATCCTATTGAACGTTTAATGAATTTCCTTTTAATAAAATCATTATTTAACAATGCTGGCACGATTTTTCCAATTAAGTCTTTTAGGAATTTTTTATCTTCATCATTTCCTTCTATTTTTAGATAATTTAAAATACTTTCAACAATGTGATTTGTAATTTCATCATCAGTTGCAAGATGCTTAATAAGTTCAGAAGAGTTTTTAATTAGATTATCTTGAATTCTAGTAATTGTTTTAGATATTAAGTCCCCAAGGTTGCTTGATTCAATTTTTGCTCCTGCTAGAAAATCATTAACTACGGCTTCAATTAGGTTTTTAATTTCTGGAAAATCAATGAATTTTTTACTTATTAATTCCAACTTATCTTTTGGAATAATATCTTGTTTAAGGTCATCTCAATTAAATTTCTTAATTTGATCCTTCAAAACATCATATACAAAATCAACTAATGCTTCTTTTCCATTTTTATTATCTGCTTTTATTGATCCATTTAGAGCTTTTAAAGCATTGTAAATCAATTTTTCTAAATTCTTTAGGAATTGTTCGTGTTCCTTATCTTGTTTAGTTTTATCTACTTCGTTGAATGTTGTAGAATTGCTTTTAGCAGAGAATGCTTGTTTAAATTTATCAAAATTATCAACTATATATGAATAAGCGAAATTCAATTCATTATACAAGACATTTTTATCAATATTTTTATCTAATATTGTTTCAAATAATTCACTTAAATTTTCTGTTGTTAAATAGTTATGGTATTCAATCTTTTTAATAGATTCAAGAAGTTTGGATGCCAAAGCATTTCCATTGAAATGACCATTATTGTCAAGTAAATCAACATCAACAAACAAATCAATGAAGTCTCCCAATATATTGCTAAATACGTTCATTGAACCTAGTTTGTTAATTAATCTAGTCAAAAGATTTGCAATTCCTTGAATTTGAGCGCTACTTGAACTAATTGAATATTTCTTGTTGAATAAATTCAATGCATATTCAATAGATTTGTTAATTAAGTTTTTGTTAGAAGTCGTTTCATTTACTGCGTTTTTAATAAATGCTTTAAATTTATCTTTATTTACTGACAATAAATGTTTAAATAAACCATTGAAATCACTAATAGAAGCTCATTGATTGTTATCGCCTAAATCTATAAATGAATTCAATAATGTTAGTATAAATTCTTTTGTATTGTTTGAATCAAAAATCACCTTCATTAATGTTTCAACTATTTCATTGCCTAATTCAGAAGCTTTTGAATCAATTTTTAGAATTTTTTCTTTTATTTTTAATAAATTGTGTTTGATATATTGCTTGAATTTATTACTTTGCAAGAAAATATCAACTGAATTTTTTAATTCATCTTTAATTTCTTTCTTTGCTTTATTATCTAATATTGAAAATACGCATTTAAACAAATTAAAATAAACATTGATATTTACGTTGATTTGTCCATCAAATCCAACTATATTTGTGCTTGAAGAATCTAAATTTTTATGTTCAGGAAGTACTGGAGATTCATTGCTATAATCTGGAATAATATTTTCAAAGTTCTTGATATTTTTTAATAATAATAATGCAATATTTTTTATATCTTCAGATTTTAAAGATTTAACAAATTCTATGATTTTGTCGTAGTTTAAATTATTAATAATGCTTACAATATTAATTAAATTATTTCAATCGTACTTTTTAAATTCAAATTGATTCTTGTTTAGATTATTGACAGAGTAGTCTAAAACATTTTCAATTAAGCCATCTAGAATATTAAATTCTGGCAATTTTTCAATGCCTAGCGAAATTAATTTTAGTAATGTTTCTTTTGTATTAACAGGCCATGTAATATTCTTTAGATTTTGTTCCATCAATGAAACTAATAATTTTGCTATTGAATTTTTAATATCAATACCTAAGGTTTTGTTATTGATGAATTTATTAAATATTTCTTTTATTTTTTTAATAACATATTCTTTATTATTTGAAATAAATTTGCTTATTCATTGAGAAATATTATTTAAATCTTTTAAATTATTATCATCAAATAACTTTTTAATTAGTTCTTTTATTAAAGGTAAAAGATCTTCATTGTAACTTGTTTCAACAAATGATTTTATATATGAAATTATTTGTTGAAGGTTTTCTTGTACATCGCCCTTAAAGCTTGAAATATTTATTTCATCAATGTATTTTAATATGATTTCTTTAATATTTATTTTGCTTGCTATGTAATTTATAAATGTTTCTTTTTCTAAAACAATCTTTAATATTTTAATTAGTTCGACATCTTTTATTTGGTCAATTTCTTGTTCTTCATATTTCTTTGTTAAGAATTGTTGAACATCCTTAATTAATTCACCATTATTAAAACTAAAATAATTCTTAATCGTTTTTGTAATAGATTCAAGAATAGAAGCTCAAGAATATTTATTTATTGACTTCAATTCATTTGTTAATTTAATTTTTAAGTTTTTATATAAATTAGATTTCAATGAAGTAGGTAAAATCTTGTTGAAATAATTAGTTAATTGGTTAACTTCATCAGAATTCAATTTTGTTTTTGCTTCAATTTCTAAAAACGCAACTAAAAATTCAACTAATTTTTGGGTTATATCTTTATTTGATAAAACATTTTCTAGCAATTGTTCTAACGCATCAAAGCCCTTGTTGTCTGCAAGATTTTTCAATATTTTATTGATAAATTCGGGCAATGTTTGAGCATCATATTCACTGCCGTTTTCAATTAAATTTTCAATAAATTCGAAAACTTTGTCAATTAATTTATTATCAATAATCTTATCGAATTTATCGACAATAAAATTGGCAAGATTATTATCATTTGTATTGACTTTATTTAGAACTTCATTATGAACATTTTTTAACTCATTATTAATTATTTTTGTTAAATATTGTTTTAAATTTTCATTTTTTAATAACTTTTGAACTTCTGGTTTCAACTCATTGATAATTGTAGATTTAGATGAAGCTAGTTTATTTCATAATTTTTTAATTAGTAAGAAGATTTGCTCAAAATTTAAATCATTGTCTAATTTAATTGGTTTTGAACCTTCATTTTGTTTTTGTGTTGTTGTGTCGTTGTTTGCTAAAACAACATTTGCAAATTCATTTAAATTCTTGATTAATATCAAAAATAATGTTTTTACATTTTCTTCATTTAATGTATCTATCAATGAATTTAATTGTTCGTAGTTAAATAAATGCTTAATTAGATTTTTTATATCTAAAGCATTGCTTCAACTAATTTTGTCTAAATTAAAACCATTATTTTCAATGTATTTAGAAATATATGAAGCAAATGTTGATACTAACAAGTCAACATTTTGTTTTTGCAAGGCAAAATCAATCAATTTTGATACTAATTTTTCAATATTGCTTTCACTATCTGCTCCAGCAAGAAATTGAGGATATTGTTTCTTTAATAATTTAAATAAACCACTTTTAATTTCTTCGTTGCTTTTTATTTCTTTATCTAAGAAATTATTTAAAATTTCTTTTACTATTCCTACATTTTTTGAAATTACGTTTGCAACAAATTCATTAATGTTTTTAGATTCTATAACCTTATTATCTATTGCAGAAAGTTCAATAATCGATTTAATTTTTGGAAGTAGTTCGTTTTCTCATTGACTATCTATGTAATTTCTAGTTTTGGTGATTAAAGTTAGTAATAGTGAATTTAATTCAGCATCGTTAGATAGTTTGTTGTCTTTAAATGAATCCAAGTAATTTAATATAATAGATTTAATGTCAAATTTTTTAAAGACGTAGTTAACAAGTTCGGATTTAGAAGCAAGAACAGAAAGTGTTTTTATTAATTCAGAAAATTTTATTCCTTCTGATTTATCATCTCTTTCGAGTAGTAAATCAACAAAATTATTAATTAAATCCTTATTTTTTTCAATGTTGAAGTAGTCACTAAATATTGATTTTAAATAGTTTTTCAATTTATCAAATGTGTCAATTTTTTTATTATCTAAAATTTGAATTTTTTCATCAAAATTTTCAATAAGTTTAGTTACTAAATTGCTTTTAGTAATTGAATTGATTAATTTAATTAAATAATTGCTTATTTCTTTAATTTCTTTATCATTTAGTTCAGTATTTGTTTCTTTTTTTAATCAAATTGCGCCCAATTCAACTGCTTTATCAATAAATTTGGTGTTAGTTGCAATTTCTGATATCACCGTTTTTAATAAATCAATTGAACCATTCTTTTTAGAATTTTTTAAGAACGAAACCAAAAAATTATAGCTAGACGAGAAATCATATTCATTATTTCCACTAATAAAATCGTTTAAAATATTTTTTAAACAACCTAAAACTTTCTCACTTCTTATTGTTGAAATTAGTTGATCTCAAACATTTTCAACAAATTTTTGAATTTCTTCTTCATTGTTGAATATCTTTTTGCAATTAATTGATTTAATGAAAGCAACGATAGGCTGAATAACAACATAATTTCCAATTTGATCAATTAATTGGCTGAACAAACTATTATTCTTAAATAATTCATTTACTAGAAGCGAGATAATATCTTTAAATATTTCGCTACCAAATTCGTTTAAATTCTTCTTTGCATTTAATGCTATATTTTTATAAAATCTTAATATGTTTTCTCTTGAATCTTTATCGATTAATTTGCCTATGTTTTTTGGCATTAATGCATTATTTTTAATTGCATATTTTAGGGCTAAATGTTTTAATTCTTGTTTGTAGTCGTTAATATTTTGTATTTTTGGTTCAAACGATAGGATTTTAAATAAAAGCGAGTTATCCCTATTTAGGTCGTATTTATCAAAAAGAATTCCATTCAGAATTTCTGAAATTAAATCCCTATTTTGGTGGTCATCATCTAGTTTAGAATTCATTAATATTTGAATAATAATGTTTGATATTTGAGATAAACCATCGAAGTTTGAAATGTTGTCAAAAAATGTTGAAACAAATAAAGAAATATTTGGTTTTTCAAGTAAATCTAAATCATATGATTTGTATAAAGAATCAACAATTTTTCCGGTCAAGTAGTTTGCAAGCTTTCTATCTTTTGAAATTTCTTTAATTATTAGGCTAACTAAATCATTTACATCTTTGCTTGAATCATGAACCAATGTAGTAATAAAGTCATCAATTTTTTCAAATTTAAAATATTTATCTGAATTATTAATTAACAAATTCAACACTTTTTGAAAAATGGTGTCTAAACCTTTTTCAATATCTTTGTTATTAAAAAGATTTTTGAATAAATCAATAGTAGTTTGAGGCAATAATCTCTTTAATATTTTTTTAATGAATACATCTGAAAATATAGCCTTAAATAATTTTGCATATTCACTTCTTTTAAGATCTTGACTTAAAGATTTGTTATAGAAAATTTCTTTTAAAATTTCATAAATATTTTGTTTAGAAGTAGTTGTTGAATAAACAATTTTTTTAATTTCTTCAAAGTTTAGACCTGCATATGATTTTTTATTAATAGCATTATTTAAAGCATTGTTGATATCTTGAAACGATTTGCTTAAATTTTTTGAATCAATAAAGAAATTCAAAAAATTAATAATAGATTTTTTACTTTCTTTATTCTTTAAAGATTCTAATAAACACTTATTAAAGCTGTTTAAATCTTTAGGGTCTAAGCCTATTAATCCGAATAGTATTGAAAATGTATTCAAAATTTCTTTATCTTCAATGTTTTTGTTTGAATTTAACATTGATTTTAATTGAAGGATTATATGGTTTGAATTGTATGAAGAATTAATATTTTTGATATCTGCATTTTCTTCGAATGTATATTCTTTTTCAAAAGAATCTACATTGTTGCCATTTATTCCTAAAATCATTGAAAAAATTTCGGAAGGAGTTTTGCTAAATTCAAGTGATTGGCTAAATTCATTTAGATTAGAATTATTATCAACTAATTCTTTATATTTATTATTAGGCAATGACATTTTCATAAAAATATCTTGCGCTAATTTTTTGTATAAATTATTAGATGGGAAGCAGTCAAAGAAATTATTGTTTAGTTTATCGGCATTATCTTTGAGATTTTTAATGTCATTATTAATGTTTAAATAGTTTAGCTTGTTTGTTTTGGCAATGTTTGCTAATGATTTGTTTAATTCTTCTATGCAATCATTAGTCAATGAAATATTGTATTGCTTGTCAATTAATTTTGTTAATTTCAAATAAGGCGATATATAACCCACAAGATTAATATTTGCCTTAGGATTATATTGTTTAATGATTGAAATTAATTTTGAATATGAATCTTCAAAATTTTTACAATTTCTTTTAAATTCAAGAAGAATTTTTGAAAAATATTTCAAAATTTCTTCTTTATTTTTATTTTCTCTTAAAAGATCAAACGCTTCTTTAAAAAATGATGATTGTTCAAAAAGGTCATTGAATCCCAAAGAAATTGTAATCAGGTTTGCTTGTTTTATATTTTTAATTAGCTCTTGTAATTTTTTATTAGAAAAATCACCAAATAGATTATTCAATTTGGTACTATTAAATTTTGAAAGTAAATTTTGGTTATAGGAAATAACTTGATTTAAGTGAGCTGATTCCTTTTTGTCATCTAGTGAAAGTAATGAAATTCAATCATTAACATTTGAAAGTGGCAAACCGTAGTTATAATAAGTTGATAATGTTGTTTGTTTATCAGATAATAGTTTTATAAAATTAGCAATGTATGAAGGATACGATGTTCCTAAAACTAAATCGTTTTTGCTATCATATTCATTGTTGAAATAGTAATTTGAGCTGCTATTATAACCGGCAGCAAAGCCGTCTCCTAATGCAACATAATTAATTTTTTTAGAAACTTTATTTTCGCTTTGAGTTATTTTTATATCTGGCTTCTTAGTTTTGTCTTTGCATGATATAGCAACTAATGAACTAGAAGCAATTGAAGATGTAATTCCTAAAGCAACAATTGCCTTGAGGATTTTTGTACTTTTTTTCATAAATACCTCCTAACCCATAGAAATATCTTCATAAATATATTGATATCTTTGTGGTTTTGATTTTGAAAAAGTAAAAATTTTTAGAAATTGTGATATTCCTAATTGTCCTACTATCATTGTTATAATTAAAAGTATTTTTCCAGTAATGCCCAAATTTGGCGTTAATCCAATATTAAGTCCTGAGTTTCCAAAAGCTGAGAAAACTTCGAACATTACATATACCATTGGATATGACTCATTATAAACATTTTTTAAATCGCTTGGAAGTGTATGATATATTACTATTGTCATTATTAGAGTTATAAAAACTGCAATTGCAAAAATGTTAACTGCCTTAATTAAAGATTCTTTTCCTATTTGTCTATGAAAGGCATTTATGTTTTTGCGATTTTTAATTAAAGTAATTATACTCATAAAAATTATAGCCACTGTAATATTACGAATACCTCCGGCAGTTGAAACAGGAGAAAATCCAATAAACATTAAGATACCATGAACCATAATTGTTGGTTGAGTAAAATTATAATAATTTACAGTTGAAAAACCTGCTGATCTTGTTGACATAGTTTGAAAATATATTGCAAACACCTTGTCACCTATCGAACCATATTCGCTTGAGTTCCAAAAATAATTTGCCCCCTTAGCAGTTGTTTCAAAGATAAATGTTAATAATAAACCAATTAGTGATGTAAAAATGTATGTAATAATTGTAAATTTTGTAAATAATGTAAAGCAATGACGAGGATTGTCTTTCTTGATGCTTTTAATTTTTTGTCAAACATCATAGAATACAGGGAATCCCATTCCACCAATTAGAAATAAAATTGATGTTAAAAATAAAAAGTAATAATTATGATAATAAGGTTGCAAACTCTTTGTGCCAATTATATCTAATCCAGCATTATTTATTGCGCTTAGTGTATGAAATATTCCAAATCTTAGTGACATACCAAAGTTATGATATGGATTTCTTATTGATTCTTCATAAGGATTAAATGTTCATGGATTATTTTTGCTTATTGCATTTAATTGAGACATCTTTTCATCACTAAAAAGTGCTGTATTATCATAATAAAATATTAAAGAAAATATTAAAGAAAATAATATAGAAATAATAATTAAAAAACTTATTGAAATTTTTATCATTTTTTTTGTTTCATAAATATTAGAACCGCCTCTTTCAGTTTGATGAATTTGGCCACTAAATAAATTCGTTCTTAATCCAAGTAATGATTGAAAAATATAAAGTTTAAATGTAAAAATTCCAATTCCGCCAAGAATAAAACAGACAGCTATTAATATCTGTCCAAACATGTTAAATACTTCACTAACGGCAACTCCACTTAAACCGGTTGTTGTAAATGATGAAGCTGCTAACAGCAAAGCGTCAAGATAACTAATATGCTTTGAAGGGTTATGACTAACTTCTCAATATAACATCAAAGACATTAGAATCGTAAAAAGTATATAAATCAAAAAGATAAATCTTATTGTTCCAAGTTTCTTTAAAAACACAAATAAGACATTTGATTTTTTTGGATTTCCCTTTTTAATCATAGAAAGAATTTTACCACAAAGTGAAAAAATGTTATTTTATAATACTTTACCATACCGCTTCTGTGTAAAAAAACTATAATATTTAAGCTAATATATTTAAAATTCAAAATTATTTTTTAAAATATTAAAATTTGAAATGAAAAGAGGTTATTATGAGTTTGTTTAAAAAGATACGTGAAATTTGTATTATTGGTATAGGTCGTTATGGTTCGGCAGTAGCAAGAACATTATTGGCCGATAGAAATAATAATATAAGGATAGTTTTAGTCGATGAAGATGAAAAACATTTGTTGCCTTTTAAAGATGAAGTAGAAATGATCTACGTTGCAGACTGTGCTGAACAAAAAACATTAGAAAGTCTTAATATAAAAGATTTTGATGCTGTTATTGTTGGTGCTTCAAATAATATTGAAATAGTTGCTGCTTTAACTGAATGTGGTGTAAAAACTATAATAGCAAGGGCTTCAACAAAAAGACATGCCCAAGTTTTAAAGCAAATTGGTGTTAGCTCAATTGTTTGTCCTGAAGAAGAAGCCGGAAAGAAAACTGCAATGTTAGTTGCAAACCCTGCGCTTGCATTATATTCAGAAAACATGGTTGAATTGCAAGACGGATTTGTTTCTGCTTCAATATATGTTAAAAACCCTGCTGTTGCAAACAAAAAAATTAGTGAATTAAATTTCAGAAATAAATACAATGTTTTAGTAACTATGATTAAGAGAGATAAACATTCATACATTCCTGAAGGCGATTTTGAAATAAAATTAGATGATTTAATAACTCTTATTGGTCAAATTGAAGATATTATTCAAGTTATTGAAATTTGCGCAATGAACGAAAAATAACTTAAAACAAGAACAAAAAAATAGCAAATTTTTTCTAGTTTGCTATTTTTAATTTTAATTTGTAAATATAGTAAAATTATAAATATTATCAATTTACAAAAGGAGATTAAATGGCTATTTTAGACAATGAGCTTCTTAAAAAATTAGAAGCAAAATACGAATCTAATTTAAATAACAAAATTATTGAAAATGCTATTTTTAAAAATGGTATTAAAGCTTCATCAACAAATAATCAAGTTGTAAGAAAACACGATTTTCAATTTTCTGTTGAAGTAAAACCTGGCTCAATAACTAACCAAAAACATTCAGGTAGATGTTGAATTTTTGCAGAACTAAATGCAGCAAGAGCAAAGGTTATGAAGGATTTAAACATTGAAGATTTAGAATTTTCACAAAATTTTATTCATTTTTATGACAAATTAGAAAAAGCAAATGCATATTTAACATGAATTGCAACAACTGGTTTGGAATTAGATAATGAAGATAGATTATTTAGAAGATATAATGAAAACCCTATTTCAGATGGTGGATATTTTGAATTCTTTTTAAATATTGCTAGCAAATATGGAATTGTTCCAAAATCTGCAATGCCAGAATCATTCCATAGTGAAGCAACAGACGATATGGTGGAACAAATTAATTGAAGACTTAAAGCATATACATTCAGAATGCGTGAAGCATATAAAAAGAATCATAATATGAGTGAAGTTGAAGCAATTCAACAACATGCTTTGGAAGACGTTTACAACATTTTAGTTAAGTCCCTAGGTATGCCTCCAAAAACATTTATTTTTGAATACTATGATAAAGATAAAAAATTTGTAAGATTGCCAGAAATGACACCAATAAAGTTTTTTGAAACATACATTAAACAATTTGTTGAAAATAAGGTTGATGTAATTAGCGACCCAAGAGAAATTTATCCTTACTATAGACAATTGGTTGCTCCTTCGGTTTTCAATTCGGTTGGTGGTATTGAATATAAAATGTTAAATATACCAATGAATGAATTAAAGGCAGCAGCAATTAAACAATTGCAATCTGGTCAATCAATTTGATTTGGTTGCGATGTTACAACTTTTTATTCAAAAGAAGGAATTTTTGATACTAAATTATATGATTATGATTTAACTTTAACAGAAACTCCAAAAGAATTTAATAAGAAGGAAAAATTCGAATCTCGTGCTTCAGTTATTAGCCATGCCATGAACTTAATTGGTGTTAATTTAGATGAAAAAGGAAACCCAATTAATTGAAAAGTTGAAAATAGTTGAGGAACTGAAGTTGGTAAAAAAGGCTATTGATCAATGAGCGATTCTTGATTTGAAGAATATGTTTACATGCTAATTGTTGACAAGGAATTTTTATCAAAAGAAGCAATTGAAGGTCTTTCAAAAGAAGCTATAGAAATTTCTCCATACGACCCATTATGCGAAGAATAATAAGTTTAAAAAAGTTGCACATCGCAACTTTTTTTATTAAAAATAATAATTTATATAATTATTAACTTAATACATTTATAATTAATACATTAATGTATTAGAAAGAGGAAAAATGTTAGGTGTGGACTTAACCGATATTAGACGATTTAAAGAATATAGTGAAACGCTTTTGATTAAGTTTTTACATCCGATTGAAATTGAACAATTTAAAAAGGTTTCGATAACAAAACGTGCTGATTTTCTTGCGACTAGATGAGCAATTAAAGAGGCAATCTTTAAAGCCGACAATCAATATTATTCATTTAACCAAATAAACATAACAAAAAATGAAGTTGGACGCTACGTTTTTAAAGATTTTGAAATTTCAACCTCTAATGAAGGAAGATACATAATTGCAGTAGTTTTTAAAAGATAGAACAAATAGTAGGAGTTATTTATGAATTTAAGAACAAGAATATTTTTTAGATTTTTTGGTTTGTTACATTGCATATCGTTTTTATGTGCAAAATCAAGACGTGATTTTAAAATGCCTGATTATTACAAAGTAGTAGAAAAAAACGACTTTTTGCAACAAAAAAGTAATGCCATTTTAAACCATTTCAATATTTCTGTTAATGTTGAAGGGTTTGAGAATGTTCCTAATGGACCATGTTTAATTATTCCAAACCATTCAACGTATTTAGATCCACTAATAATGTGATCAGCATTATGAAACCATAGAGATGGTTCAAAACAAAGTAAATTGGTAAATTTCGTTGCAAGAAAAGAAATAACAAAAAAGAAAATGGTTAAAAGAATAGCAGATTTAGGTCAAACTTATTATATTGATACTCAAAACCCTAGAGAAGCATTAGCAACCTTGAAAGATTTTGGCCAATTTATTAAAAGAAATAAATCATGCGGCGTTATTTTTGCTGAAGGAACAAGAACAAAAGATGGAAAATTAGGTGAATTTCATAGTGGCGCCTTTAAACTTGCTCAATCTTCATATTTAACAATTGTTCCAGCAACAATAAATAATGCAGCAAACGCATTGGATTGAAATAGAAAGGAAAAATTAGAAGTTAAGGTGACATTTCATAAGCCAATGAAGCCAATTCAATTTCAATCACTTGATAGCCGTGTATTAGCAGAACAAGTTAAATCAATAGTTGCATCAGATTATATTGATCAAACAATTACATCAAATGAAACAATCAAAAACAAATATTCAAAACGAGACAATGTAAAAGAAGTTAAGAGATAAAATATGTCCGATTTAGAAAAACCAATTATTAGAGAAATTTCAATAAAAGAAGAACCAAACAAAGATCAAAACAATCAAACTAATTCATCAATTTTTAAAGAAAAACACGTATTTAAATTAAAAAATTTTGATGGTCCGCTTGATTTGTTGGTTTCTTTAATTAAAGAGAAAAATATAAGCATTTTTGATGTTGACTTATCTGAATTAGCAAATCAATATCTTGAAATAATTCATAGTCTTCAATCTTATGAAGTTGATATAGCAAGTGAATATCTAGTTATGGCTGCACATTTATTACAATTGAAAGCTAGAATGGTATTGCAAGATCCAGAAGTTGAAGAAGAAATTAGACAAGAAAAAAAGAGATTGCTCGAACAGATTGCTGAATATGAAAAATTCAAAGAAATTTCTTTACTTTTACGTGAACAAGAAGAAAAAAGATCTAGATTGTTTTCAAAAGAACCAGAAGACACACAAGATTTCGTAAGAGATATTGATGGTTCGATTCTTGACGGGCATTCCAATAGTTCAAAATTAGTTGTAACTCTAAGAAAAATGTTTGAAAGAACATATGCTGAACTTATTAGAAATATTACTATTTCAACAATAGCAGTGAGTCCCGAAGAACAAAAAGAAAGAATTATTAAATTATTTAAAACAAAGTTGCAAGTTACCTTTAAAGATGTTTTCTCTGTTCCAAGTTTGGGACACTTTGTTATTACTTTATTGGCAGCATTGGATTTAGTAAGACAACAAATTATTGAAATTTATCAAGAAGAAAATGAAGGTGAAATTCATTTTAAGAAAGGGGCTTTATATGAAGAATAAAATTATTGAAGCTTTGCTATTTATTCAAGGCTCTGAAGGGTTAAGCAGTGAACAAGCACAACAAGTTTTCAAATTAAATACAATTCAAGAAGCAAGACGATTATTAAGGGATTTTAAAACATACTTTAATAACTTGGAAAGAGGAATAAAAGTATTGGAATTCAATGATGTATTCAAGTTTTTAACTATTGAAAGCGTCAAAGATTATATTAGTGATTTAGTAACTATTACTAGAAAACAAAGATTATCAAATGCTGCAATTGAAGTTGCGGGTATTGTTGCTTATAAACAACCTATAACAAGAGGCATGATTTCAAACATAAGAGGTGTTGTAAGCGATGGAATTGTAAGTAGTTTATTAGAAAAAGGAATTATTGAAGAGGTTGGAGTTGCTCAAACTCCAGGCAACCCTATTTTATATGGAATTACTAATAAATTCTATGATTATTTCAAAATAAAGACCTTAGGGGAATTGCCTCCTTTTCCTGAATTTAATAAATATAGCAGTGAAGAAGAGGAAGAAATTGATAAAGACTTCAATTTGTTTGATTCTCAAAGACAAGAAACAGACAATGATGGAGTTTTAATTTCTAATGAAGGGGCAGAATATGATAAATTTTAAGGCTAAAAAGGATGACGAAGGTCGTATCCTTTTTAAATATTTGGTAAAGGTTTGCGACAATGTTCCAATATCTAGAATAGAGCGACTTTTTAGAGAAAAGGATATCAAGGTAAATAATATACGTGTAAACGATAAGCAATACAAAGTCAAAGAAGGAGATTTAATTTCAATTTATGGATTAATTGAAACTTCCAAACATATTGATAGCAAAGCTAATACAAACATAAATTTTAAAGTTATTTACGAAGATAAAAATCTTTTAATTATTGACAAGGCGCCAAATATTGCAATGCATTCTGAAGAAAATTGTCTAGATGATCAAGTTTTAAAATATTTAAATTTTAAAAAAGTTTCTTCGTTTAGGCCCGCTTCAATTGGTAGAATAGATAAAAAAACTAGCGGTTTGGTTGTTTATGCAAAAAACTACAAAACTCTAGTTGAATTTGAAAACAAACAAAATAAATTTGACAAAATTTACCAATTTGTAAGTGATTTTGATGAAGATGTTTTGGATATAACAGTAAGACTAAAAAAAGATATTAAAAACGAAAAAATGATTGTCGATAATAATTTTGGCGTTAAAGCACAGACAATATTTTATAAAGAAGGAAATAGAAAATTTGCCAAGATAATAACAGGTAAAAAGCACCAAATTAGGGCGACATTAGCATATTTAAAAACACCTATTTTAGGAGATATTAAATACGGCGGAAAATTTGCTAAAAGAATGTATTTGCATTCATATTCAATAAAATTTCATGATCTTTCTGATGATTTTAAAGAATATAATGAACAAGTATTCATTTGCTATCCAAAATGATAAAAACGATTAAGGAGTTAACATGGAAAAAGAAGAGATTAAAAAATTGGCTAATGATCTTTTATTTGAACCAAGTGAAGAAGTTATTAAATTAACTAATAACTTACTTAATTCAATAGAAAAAGGTTTAAAAGATTTAGAGGAATTTGACTTACAAGACATTAAACCGATGTCGCATATCAATGAATCACCCATTTGTTTCGAACAACTAAGAAGTGATGAACCGGATAAAAGTTTTTATTTGAATAAACAAGATTTATTAAATAATGCATCTGAACATGATGAAAATTATGTAATTATGAAGAAGGTAATAAATGATAAATAAAGGTAATTTAAAAGAAGCAATTTTACAATTAAATGCTGATAAAAATAATGCGGTATCCTTTGTTTTTAACGATGCTCAATTAAAAGAACAAGGACCGCTTGCAAATTGTGTTTTTACAATTAAGGACAATTATGCAGATTTGAATGTTATTGAAAGCGCTTCAAGCAGATTTCTTTCTAATTTTAGACCTTCATATCAATCTACAATCTTAAAATTATTAAATGAAGCAGGAGCAACGTGCGTTGCAAGAACGAATTTAGATGAATTTGGTATGGGTGGCAGTGGAGAATATTCATGCAATGGATTAATAAAAAATCCTTTGAATAGTAATTATTTAGTTGGAGGTTCTTCATCAGGTGCCGCTGCAACTTTTTCTAAAAATATCTCGTTTGCAATTGGATCTGACACAGGCGATTCAGTTAGAAAACCTGCATCAAATATTGGTGTGGTTGGATTTAAACCAAGTTATGGTGCTATAAGCCGTTATGGATTGTCTGCTTTTGCTTCGTCATTAGATACAGTTGCATATTTTACACATAATGTTCAAGATGCAATAATTCTTTCATCAGTATTGTTTAAGCAAGATTTAAAACATGATTTGACATCAGTTGATTTGTCGTTTAATGCAGATAGTGTTATTGAAACAAAACCACATAAAATTGCTTATTTGGATTGTTTTGACAAATTGGATATATCTGTTGCTGATGCATATAAAAAATTATTGAATAAGCTAGAAAAAATGAATATTGAACTGGTAAAAATTTCAATAAATGAAAAGTTATTACAAGCCATCGATACTGTTTATAAGGTTATTGCTTTTTCAGAATCTTCATCTAATTTAGCAAATCTTGTAGGTGTTGAATTTGGCAATAGAATTACTGGCAATTCTTGAGAAGAAACATTTAATTTATCAAGGACAGAAGGTATTGGAAAAATGGTTCAATCAAGAATGATTTTAGGATCTTATTTTTTAGAAGATAATAATCAAATAAAGTATTTTGTTAAGGCTAAAAAAATGAGAAGGTATTTAAGCAATTATTTCACTAATGTTCATAAAAGCGCAGATATATTTATCTATCCAGCATCTAACATGTCCGCTCCTCTTATTGGAAAATCATATGAACCAAGTTATATGGATTATATTTTAACAAATTCTAATTTGACGGGAAATCCTTCTTTGACATTAAAATTAGGAATTGACAAAAATAATAATCTTCCATTTAATTTGGCAATTGATGGAGAAATATATAAAGATTGCAAGATGCTAAGTCATGCATTATTTATTGAAAGAGTATTGGAAGGTGAAAAAAATGAATAATGATTGAGAAATAGTTATAGGAATTGAAATTCATATTGAATTAAATACCAAGACAAAAATGTTTTCTCCAGTTCAAAATGAATACGGAGATGAAGCCAATACAAATGTTTCAAATATTGACCTTGCCTATCCTGGAAGTTTGCCTCTTTTAAATTCTGCTGCCATAATTAAGGCAATTAAATTAGCCAAGGCGCTAGAAATGGAAATAGATCCATTAGTTAGATTTGACAGGAAAAATTATTTTTATCCCGATTTAACAAAAGGATATCAAATTACTCAACAATTTCATCCAATTGGTAAAAATGGTTTGATAAGGGCAAAGGTTCAAAATTCATGAAAAACTTTTTCTATTGAAAGAATTCATATGGAAGAAGATACTGCCAAATCAATTCACGAAAATGGTTTGACATATTTAAACTATAATAGATGTGGAGTCCCTTTAATTGAAATAGTTTCTAATCCCGTAATGCATAGTGCAGAGGATGCAATGGCTTACGTTGAAGCTATAAGACAAACAGCATTAGTTTTAGACATTAGTGATGCAAAATTAAATGAAGGAAGTTTAAGGACTGATGTTAATTTATCAGTAAGAAGAAAGGGAGAAAACATTCTTAATACTCGTACGGAAATAAAAAATTTAAATTCTCTTTCAAATATTAAAAAAGCAATTGAATATGAATCTAATTTCCAAATTCAAATGTATGAAAACAATTTAACTTTTAATCAAGAAACAAAGCGTTTTGATGAAAAAATGCAAAAGACTGTTTCAATGCGTTCGAAAGGTGATGCAATTAGTTACAAATATTTCCCAGATCCAAATTTACCTTATATTCAATTGCAACAAAAATTTATAGATGATATTAAAATAGAAGAAATACCATATCAAAAAGAAATAAGATATCTAAAAAATGGCTTAAATCAAGTTCAAATATCTCAATTATTGAATAATATTTCTTATGCAAAATATATTGATAGTATAAACTCAAAAGATTTTAAAAAAGCCACAAATGTTTTCTTTTCTGACATAGTTACCTATTTAAATAGTAACAATAAGAATATTGAAAATTTATCATTTAAAGCAGATGATGCAAGAAAGGTTGTCGAATATTTAATTGATAATAGGATTAATAAAAATAATATTGCTGAAATTATCAAAATTAAAGATCAAAATCCAAATTTAAATATTGAATCTATTATTAAAGACAATGGCTTTTTAATTGAAATAAAAGAAATTGATTTGAACGAACTAGTTCAAGAAATATTAAATGAAAATCCAAACTTGGAAACTGAATTTAAAAACAATTATAACCGTGCCCAAAAATTTATGCAAGGTCAAATAATGAAAAAAACAATGGGAAAGGCAAATCTAAAAAATCTTGATGAAATAATCAAAAATAGGTTCAATAATGAGTAAAAAATGAAATCCAAATAAGCTACCTACGGGTTGAAAAGTTGATGAAGATCAAGCAAATAGAAAACCAGAAACTATTTCTTCAACCCAAATTATTGCAAGAAAAAAACGAAACAATATTTTAGAAAAAATTCTTAAGTGAATTATTTACGGAATTATTTTTATAGCAATACCTAAATTTATTAAAAGTTCAAAGACAAAAGGAAAAGAAATTGTAAATTCTGCATATGAAAAAATAAATTCAAATGAATTTTCTTTTGTCCCCGCTGGATATGCAATGTATCTATTTTTGTCTTTTATTCCAATCTCAATATTGATGCTTGGAATTATTGGCTCGGTTGATGAAAAGTATGAAGTGGTGTTAAAGAACGTAATTTTGGGTCAAATTATTCCCGGAATTTCAGAAGTAATTCCATCATTTAGTACATTATGAGGTTCTACAGGCAATGCAATTGCTTTAATCTTATTTGCATTATCAGTACTAATTTTAGCATCGAAGGGATATTCAAAATTTATTTTTTCAATAGATGCTTTATATGAACACAAATCTCCATTCAGAGCGTGAAAATCGAGAATTAAAGGATTCATAGTAAGTGTCTTTGTCTGCTTGATATTGTCTTTAATTTTACTAGGGCTAACAGCCTTTATGGTATTTTTAATTGATAAAGTAGGGCTTGGAAAATTTGATGCCGATATTAAAAAAATGAAATTAGCAGATTTTAATTTAACTACAGAATTTTATTTAATATATTGATTTACAACAATTTTGTTTTTACCTGTATTTACTTATTTAGGATTTCTATTTTATTTTGTATATGCCCCAAATTTTAAATTGAAAATGTCGCAAGCACATCCGGGGGCTTTGATAGCAGCAATACCAACAGCAATATTTATTTTGATTTTTGGTTCATTAACATCCTTAATTCCTTATAAAAAATTTGGGATAGTTGCTTCATTTATGTATATTATTTTATTATTGAGTTTTACTTCTTATTTTATTTATATGGGAATTATTGTTAATTCAAGTTTTTATAAAACTTTTATTAATTTACCAACTGTAGAAAAACATTCATTTTGAAAAAAACAAAAAATCTCAATTTAAAGTCATTTGATTTTTTCGCTAATTTTTCATTATTTATAGTTATTAACGACATTTAAAATATAATTATTATAATAATTAATAAAGCAAAGGAAATATATGAATAAATTACCCGGAGGGTTAAGACACTATATTTATTGGGCTCGGTCACAGACAAATATTTCATATCGTAAATGAAACTCAAAACGTATTGCGTTTGTGGGTGTTTTGATTGCTATTTCAGTTGTCTTTTTTCTAATAAGTGTTAGAATCATGCCCATAAGTGCCTTACCAAGTATAAAATTTAGCTTTATTGGTTTGCCAATAAAAATAACTGGTTTTATTTTTGGACCAATTGTTGGTATGATAACCGGGATATTGGCGGATATAATTTCATTTGCTCTAGTTCCAACTTACTATAATGTTTTGTATACAATAGCGGTTGCGGTATCAGGGTTTGTTCCCGGGTTTGCTGCGTACTATTTTTTCAATATTAATGAATTGTTTTTTTCAAGAACGTATAGAATTTTTAAATACAAAGAAACAATTGATTATTTTAAAGAACAATATGATATTGCACTTACAATAGGTAACTCCGAAGATATGCAATATTTTTCAGAAAAAATTGCTTTTTATGAAGTCAAGATCATAATTTTAGAATGTAAGAAAAAACCAATTGCCATGATTAATTTTTCATTTATTTCAACATTGATAATATTAGGTATTCAGGTGTTGATAATTCTTGCAATTTTTTCTAACCTAGATCCTTCAATATTTGAAAAAAATAGATTTGTTAAAAACAAAAAATTCTATATGTTCTTAACTTCTATAGGATTTATAGGAATGATGATTTTTGTTTCTCTATATAGAATATTTTTAAAGAAAAAATTTGAAACCTTTTTAGAAATAATGGCAATAATTTCATTTAGCGCTGTTTTAGAAATAGTAAATGTTATTTTATTGGCGTGAGCAGATACTTCTACATTAAAGACAGATTTCTGGGTTAATTTTACTGCTCAAAGTCTCTTTAGCCCCGTTAAAATATTTTTTAATTTAGCAATAATATTGGCAACGTATAAAGTTGTTGCACCTTTAATTAAATCAAAAGAAGGTGGACATTTATAATGAAAAAAATAGCAATAAGTTCTTTAAAAGTTACCAATTTAAAAAGTATTAATGTTGTAAATGAACAATTTAAAAACTTAATTAATAATCTATCTTTTACACTTAATGCAAACAATATATTGGGTTTTGTAGGTGGAGATAGTGAAACAAATAATTTAATATTTAATTATTTAACTAATAATACAATAAATCCTAAAAGATATCAAGGTTACATTGAATTTCAAACAAACTTAGGTGAATATTCTTCAATATATAAGAACTCAAATTATCAAAATAACATAGCTTTTGGTTTTTATAATGATATTTTAAACAATGATTTAAATGAAGATAATAGTTATAGAAATATAAAAAAATATATTAACCACGGAGCAGTTATAGGGGCACTAACGGAAGAATTTGTTTCTCAGTGAAAACCAGTTTTTCAAGATTATAAATTATTGCTTCAAAGCGAATTTGCAAAGTTACAATATGGTGTTGAAATTGAACTTAAAAGCAAATTAGACGATTTATTCAACGAATTAAATCAGTTTGATTTGCGAAATATCGACGCTAAAAATTTAAAATTTGATACTTTTTTTAATAAATTTAAAGATATAGAAATTTTATATAGAACCCATGAAATGAAACTATTCGCATTTGTTAATGCAACTATAGCAAAATATAATAAGGGGTTATCGTTTTTAAAACGCAAGGAATATTTAGACAGTAAAAAGGAATACGAAGAGGTAATAAAAAAAATTTATAAGCCTTTTTCAAAGCCTAATAGATTTTTAAATCGAGTAAGATTTTTTTCTAAACTTTTAGAAACAAAAAAATCTTCAAAATCATCTATTAAAAAATTTGTAAATAAATTAAGGCGTGATTTAAATATTGAAAACTATTATTACAAATATGAATTAAAGAAAACAATAAAACAATCTTCGTTTATTCACTTTTATAAAAATCTATATTTTAATGGTCTATTATTGAAATTTATAAATAAAAATTTAAAGGCCATATTAGGGCTAAAAGAAGCAAAATCGTTTGAATTGTTGTATGAAATTTTTGATTTAAAAAACAATATTACAAATGAAGTTAATGTAATTTCAAACATTGATAATCAAAAAGAATTAAAGAATGAAATAAAGGCTGTAATAAAAAATAATTTTTTAAATGATGTGATGCCATATATTGAAAGAATTAAGGCGTCTAGTTATGAACACACTCAAGAAGTTATTAATGATGTTACAAGAGAAATTCAAACGTCTAATAACTACGATTTAAACATAGGCGAGTTTGGCCTTGCAAACAAAGCGTTTGAAAAATTAAAAACAGTTGAATCAAAATATAAAGATGCAAAAAATGAATATTTTTGAAATTATAAAACTGAAGGGAATGCCATTTTAAAGGAAACAAAAAAAGTTTTAAGAAATTTTAATAAATATTCAAAAATAAACTCACATAAATTAATCAAATTAAAACTAAAAATTTTTAATTTGATTAATTTAATAAAAATTAAGGAATTTAATAATTCAGTTCTAAATGAGCAAGTAAAAAAAATAAAAGAAAATCTACAATTTTTAAAAATTTTAAGAAATGAATTTGATTCTTATAAATATGTTGTAAGTGATTTTAAAACGGCATCAAAAAAACAAATAAAACTTTTTGTTTCAAAAATTAATATTTACAAAATTATTGCAAAGGCAAATATTGACGTTAATTTTTTAGTAGAGAAATTAGGCTCTTTAAGCGATGATGAAAAAATTAGATTAGAGTGCGAAAAAATTTTAATAAATAATCCTTCGCTAATAATAATAGGACCCAATATTTCTAAATTACCATATGAAAAGCAATGCGAAACATTGAATAAATTGAACAAATTCGTTTTAGACAATCACAAAATGGCAATTTATTTCTTAGACAATATAAATATTGCTCAAAAAATAACTACAGATTTATATATTATAAATTCATCAAAAATTATTGAGCAAGGAAAAACTAGTCAATTAATAAGAAACCCAATAAATCCTATAGTCAAAAATATGTTAAATTATAAAGATGAGAAGACTCAAGAGGATTTAAGCTTCTATATGTCTCAAGAAGACAATATGGAAAACATATACAAATATGAAATAGAGCCATACCACTTTGTTTGATGTACATGAAGCGAATTAAATAGATGAATAAACGCAAATAATGTTAAAAACAAAGAGCTAATGAGTTTATTTATTCCACAAAATACCTTTCAAAGTAAAGGAAATAAAACAATAGATAATAAGGAACCATTCGAAGAAACAACGCTTATAAATTTAATCGATGACGAAGATAACGAACTAAGGAATTTGAACATGGAAAAAACTTTTAATCATAAAATAGTTGAAAAAGACAGATGCAAAAAATGAAATGATATGAAGATTTTTCAAACTCACGATGAATCTAAAAAACCATTCACCATTATTTTGCCACCCCCAAATGTAACCGGCAATCTCCATATTGGCCATGCCCTAGACAATTATATTTCTGATACAATAATAAGGTTTAAGAAGTTACAGGGATATGATGTATTGTGACTACCAGGAACAGATCATGCAGGTATAGCAACTCAAGCAGTAGTTGAAAAGAAACTAGCTTCACAAAATATCAATAAATATCAATTAGGAAGAGAAGAATTTATTAAAAAGGTTTGAGAATGAGTAGATGAATATTCAAAAAATATTCATAACCAATGACAAAAATTAGGTTTGGCCCTTGATTATGGTTTTGAAAGATTCACATTAGATGATAATTCTAATGAAGCTGTATTAAAGGTATTTATTGAACTTTATGAAAATGGTTTAATATATCGTGACAAAAAAGCAATAAATTGAGATCCTGTATTAGAAACAGCTCTTTCTAATATAGAAGTAGTACCAAAAGAAACAAAGCAAAAGATGTACTACATAAAATATCCAATTAAAAGTTCTGAAAGCTTTCTTGAAGTAGCCACAACGAGACCCGAAACAATGTTTTCAGATGTAGCTTTAGCAATAAACCCACAAGATAGTAGAGCAAAACAATTAATAAAAGAAGTAATAATACATCCATTGACAAACAAAGAAATTCCAATTATTTTATCAAACAAAATAGATCCTAATTTTGGTAGTGGAATAATGAAAGTTTCAGCCCATGCTGTTGATGATATTGAAATTATTATTGAAAATAAACTCCCAATAAATGAATGTATCAATAAAGATGGGAAATTAAACGATTTAGCAGTTGAATTTAAGGGATTGGATCGCTTTGAAGCTAGAGAAAAAATTGCACACTTATTAAGTCAAAAAGGACTTTTAATAAAAGAAGAAGAAGTAGTTTCTAATATTGGATATAGTGAAAGATCAAAGGCTCCAATTGAAATTTTAGTTCAGCCACAATGATTTGTTAAGATGTCAGAATTTTCAAAGATGCTTTTGAAGGATTTACGCAGTGACGAAGGAGTAAAAATTTTTCCACCACGTTTTGAAAGTACACTAGAAAATTGAATGGAAAATGCTCACGATTGAACAATTTCAAGACAAATTTGGTGAGGGCATCGGATTCCCGCTTGGTACAATGAACAAAATGAAATTAAAGTTCAAATTCAAAAGCCCGAAGGAAATTGAAAACAAGACGATGATGTTTTAGATACCTGATTTAGTTCGGGACTTGCACCATTTGTGTTTTTAGGATGGCCACAAGACAAGAGCAAATTAAAACGTTATTTTCCTACAAGCTTATTAGTAACTGGATATGATATTATTTTCTTTTGAGTAGCAAGAATGTATTTCTTTAGTTTATATTTTATGGATCAAAAACCTTTTGAACATCTTTTATTGCATGGATTAGTTAGAGATTCACAAGGAAGAAAAATGTCTAAATCGCTTGGCAATGGAATTAATCCAATTCAAGTAATTGATGAATACGGTTCTGATGTTTTAAGAATGTCTTTAATTTTTAATTGCACACCTGGCCTTGATATTAATTACAGTGATGAAAAAATCCAAGGTGCTAAACTATTTGCTAATAAATTCTGAAATATAGCTAGATTTGTTGAAAATATTCCTGTTTCGATAACTGAAAAGATTGATTTTAATAAGTTGGATGAATATGATAATTGAATTTTGAATGAATATTTAACATTCAAAAAAAATATTGAAGATGCCATGGATAAATATGAGTTAACTATTATTTATAAGCATATTCAAGATTTTGTTGTTGACAAATTCTCAAATTGATATTTAGAATTTTTAAAATTTAAAGAAAATTCATATTTAATACATTATTTATTCAAACAAATATTGATTTTATTGCATCCATTTATGCCGTTTCTAACAGATTATGTTTTTGAAAAATTGTATCATGAAGAATTGCTATGCAGCAATCTTCAAACTTTTGCCTTAAATAAAGAAAGTTCAAACAATACTCCTAAAATTATTTCTTTAATTACTGCTTTAAGAAAATATAGGGAAGATAAAAAGATTTCAAAATCTATTGAATTATTCTTCTATTTGAATAATCTTGTATTCAGCAGTATAGATTTAAAAATAATTAACAAATTAAGTAATTTTTCTTGGAAAGAAAATCATGACTTTTCACTAAAAACAGAAAGTTTTGAAGTATTTATAATTCAAAGTAAGCAAGACAAGGAAAATGAAATTCTAGAATTAAATAAATTAATTGAATTTAGTCAAAAAGAAATTGAATTTAATGAAAAATTAATTAATAATCCTAAATTTATGGAAAAGGCTAACAAAAATACAATCAATGAAAAATTAGCAAACTTAAAATTGCATAAAGAAAAATTAGAGTTTTATAAAAACCAACTTAAAGAAAAACAAGAAAAAATAAATTAATTAAAAACAAGATAAGAGTTTTTTAATAAACAATTATCTTGTTTTATTTTTTATTGTCATAAAATTACATTAAAAAATTATTATTTGTCTTGAATTGCTTCTATTCCAGGTAATTTCTTTCCTTGGACAAATTCTATTGATGCTCCACCGCCGGTTGAAATAAAACTAAATTTATCTTGATAACCTAATTTGTTTATTGCTGTAACAGAGTCGCCTCCGCCAACTACTGAATAGCAATCTCTATTTTGAGCAATAGCGGTTGCAATTCCTTTTGTTCCAGCTTCAAAGTTACTGAATTCAGTTACTCCAGCTGGGCCATTTCAAAAGATAGTTTTAGCATCTTTAATAATGCTTGCAAATAATTTAATACTTTCTGGTCCAATATCTAATCCCATATTTCCGTCAGGAATATCAGGATTTTCTGGACTTGTAAATTTAGGAGTTTTGTCAGCAAATTCATCGACATAAGCATTGTCAATTGGCAAAACAATTTTATCTTTATATTTGTTTAAATATTCTTTTGCCATTTCAACTTTATCTTCTTCAAAAATAGAAGTTCCAATTTTTCTATTTAAGGCTTTTTGGAATGTATATGCCATACCACCAACAATTAAAACCTTATCTGCTAGTTCAAATAACTTTTCTAGAACTTTGATTTTATCGCTTACTTTTGCCCCACCAATTATTGCAACAAAAGGACGTTTAAATCCTACTAATAATTTTTGAAGGTGTTCAACTTCATTATTCATTAAAAATCCTAATGCTGATTCTTTAGAATATTGTGAAATTCCATATGTTGATGCATGAGCTCTGTGTGCAGTTCCAAAAGCATCATTAACATATATATCGCCTAGACTTGCTCAATATTTTGCAAGTTCTTCATCATTATTAGATTCTGCTTTATTATTTAAATCTTGAAAACGAGTATTTTCCAAAACCAAAATTTCTCCAGGTTTTAAGTTTTTAGCAGCATTTGTAACTGCTTCACTTCTATTTTCGTTAATAAATTTAACATCTCTATTTAGTAATTGCGCTAATTTTTTAGCTACAATTTCTAAAGATTTAGTCTTTTTGTCTTCTTCTGTTTTAACTCTTCCTAAATGAGATAGAATGATCAAAGAAGCATTTTTACTCAATATATATTTGATTGTTGGCAATTCTTCATCAATTCTTTTTGTGTCCATAATGGTGTTGTTTGAAATGGGAACGTTAAAATCAACTCTTAAAATAACCTTTTTGTTTTCCAATTGAAGGTCTTCAATAGTTTTCTTCATTTTATCTCCTTGTGTGTCTTGTATGTTTTATATAAATAAAAATAATATTTTGTTTAAATTTATATAATTATAAAGAAAAAAAGTTATTTTAAAATCAACTTGGGAGATTAAATGGCAAAAATTTTAAATGCTCAAAAGCAAAAGGACACAAGAACTCTTAGCTATGATCCTGAAAAAGACAGCTTGACTTTGCTAATTTCAGAGTTTCAACTTTACAATAAAAACACCAAATTTATTAATGATTATTCAGAAATGGAAAAATTCCACTATTTTAAAGAATTTAGATGAATGAAAACAATGGATGAAAAATTGCAAGATCAAGTGCAGGAATTTTCTAAAAGACAAAAGAAATTAAATGCCAAATATAAAAGCTACATTGAATATTCTAAATGATCTGAATCACCGTATGCAACAAAAACTAAACCGCTTACAAAATGAAATAGAATCATTATTATTTCAGTTTTCATTATTTTAGTAATTATCATGTTGTTAATCGTAATTGGCTTGAATAAATTGTGATAAAAAACGGAGGTATTGACATGGAAATAAAACTATTAATTGTTTTACTAGTGTTGCTAGCAGGTGTTATTATTTTCATAATTGCTAATTATTCAATTACTTGAATTAAAAACAAAAAAAATTCAAAAAGTAACAAAAAGGTTTTAGGTGAATCTACAAAAATAAGAATTATTTATCAATTAAAAGAAGCTCTTGAATTTTTATCAAAGACAAAGACTGGTGCAATTATTACTATTGAAAACAAAGATAATCTTGATTTGCTAAGAACTGATGGTGTAATATTAAATGCTAACATTTCTGCTTCTCTAATTATTTCTATTTTTAATAAACACGCTCCACTTCATGATGGCGCAATAATTATTAGAAATAACAAAATTTATTATGCTGCCACATATTATAAAATAACAAAAAAATCAATTGATAATAAATATGGCGCAAGACACCGTGCTGCTATGGGTATTTCAGAAATTTGCGATGCTACAACTATAGTTGTTAGTGAAGAAAATGGTGGAATTACAATAGCTAAAAACGGAACTTTTAGCCCTGTTTTGCTAGAAAATTTACAAGAAAGATTAGTCAATATTTTCAAGGATAATAATTAAAGAAATAAAAATTTTGAAATTATTAAAAAAGTATGGTAAAATGTATTTCGCAATTTGAAAAAAGCGATCGTTTAATTGCTCAAATTGCCGCTTAAAAATAAATATTATAGTTATATATAATATACTCTCGCTCTTTTCTTATATTTATAATAAACCTTATTTATTTTATACACATATACATACAACCCTTATTTTTTATATTTCCTTAACTTAGAACTTGAAGTTCAACTTATTATTAAATTAAATAATCAAAAGCTTATATTGTTCGATTTTTAGCGAGAGTAATCTTATTTTAAAAAATATTTCTTTCAAAATAGTAAACAAAAATGTTTGCTATTTTTTATTATTTTTGTTTAAAATATATAAGTACATAAATATCACTATTTTATGGCCAAAATTAAATCATTTACATTAAAAAAAATATTTTTTTGTTAAAATTACATTGCTATAAATGCGCGCTTTTGGACAGGACATAGTTGAAAGGCTATGCTATGGTAGGTTAAACGCGTTTAATCAAAAGATTTAATAAATATTAAAAAAATTAAAATGTAATTTATTGAAAGGAAATTATTATGGCAAAATTAGATTTTGACCGTTCAAAACCACACGTTAATATTGGTACTATCGGTCACGTTGACCACGGTAAAACTACATTAACTGCTGCTATTGCAACAGTATTAGCTAAAAAAGGTCTAGCAGAAGCTAGAGACTACGCTTCTATTGACAATGCACCAGAAGAAAAAGCACGTGGTATTACAATTAACACATCACACATTGAATATCAAACAGAAAAACGTCACTACGCACACGTTGACTGTCCAGGACACGCCGACTATGTTAAAAACATGATTACCGGTGCTGCTCAAATGGATGGAGCAATTTTAGTTGTTGCTGCTACTGATGGTCCTATGCCTCAAACTCGTGAACACATTTTACTTGCAAGACAAGTTGGTGTTCCAAAGATCGTTGTATTTTTAAACAAAATTGATATGTTTAAAGATGACGAAAGAGAAGAAATGGTTGGACTAGTAGAAATGGACGTAAGAAGCCTTCTATCAGAATATGGTTTTGATGGCGACAATGCTCCTATTATTGCTGGTTCAGCTCTTAAAGCATTACAAGGAGATCCAGAATACGAAAAAGGTATTTTGGAATTAATGGATGCTGTTGATACATACATCGAAGAACCAAAACGTGAAACAGACAAACCATTCTTAATGGCTGTAGAAGACGTATTTACAATTACTGGTCGTGGAACTGTTGCAACTGGTAGAGTTGAACGTGGTGTTTTACAATTAAACGAAGAAGTTGAAATTGTTGGATTGAAACCAACCAAGAAAACAGTTGTTACTGGTATTGAAATGTTCCGTAAGAACTTAAAAGAAGCACAAGCTGGAGATAACGCTGGATTATTGCTACGTGGAATTGACAGAAGCGAAGTTGAACGTGGACAAGTTTTAGCAAAACCTAAGACAATCGTTCCTCACACACAATTCGAAGCAACTGTTTATGTTCTTAAAAAAGAAGAAGGTGGACGTCACACACCATTCTTCCACAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACTGGTGGAATTGAATTCAAACCTGGACGTGAAATGGTTGTTCCTGGCGACAACGTAGAATTAACTGTTACTTTAATTGCTCCTATTGCTATTGAAGAAGGAACCAAGTTCTCTATTCGTGAAGGTGGAAGAACTGTTGGTGCCGGATCAGTTACAAAAATTCTTAAATAATTATCAAAATTATTAAAAAAAGATTTTTGAATTACAAAATTGGATTTGTATAAAGCAAGTCCAATTTTTTATATTTTATTTAGTATGTTTTGATTGCAAATATTGTCATTTTTGTTTTTTAATATTAATTGTTAATAAAATTAAAATTAATTGATTAAAAAAAAATAACTAAATTTCCATTTTTAATATAGAGAACAAATGAATGAAAAGAAGAAAAAAAACGCCATTCCATTGACAATTCCTAGAATGAAACATCAAGAGTGAAACACTTTCGTGCTTGAGGTTTCATTCTAGGAATTAGTGTAGAAAATGCACTAATTTTTTATTCTAATGTTAATCAAAAAAATCTTGGAATTTTTTTAAAAACTTTATTTTTTAAATGTGTTATAATGATAAATTAAATGCTTATTTTTAAGTATTTATATTGCACGGAAAGGAAAATATGATAAAGAAAAGAAGAAGAAAATTAGGATGCTTGACCTTTATTGCCGGAGGAGTTGCTGTTGCTGCTTTCGTTCTAATAATGTGTAGATTTTGCAAGAAACCTAATAAACAAATAAAAGATCGAGCCTCTTTAAATTTATTTAATAAGGCACAAAGTGAATTAAAAGAATTAATAAATTCTCCAATAGCTAAGCTATGTCAAACTGCTATTGAACAAAAAGTTTTAAACGATTCAAATAAAATAAATGAAAAGTCTACTGACGAAGAAATTTCAAAGAATACAGAATATTTAAATAGTTCAATCGCTTTGCTTAAAAATAAGTTAGATCTTGCAAAACAAGAATTAGAAAAATTCAACATGGCTATGTTTCAACTAAGATCATTAGTTAATAATAAAGATAGTAAAAGTATAGATACTCAAAATTCAATCAATGCTTTAAATAATGCTAAAAAATTGCCTCTTAATTCTATTGAACAAGTTAAGAGCGAATCTTTAACTTTGAAAAATAATATTGATGAATTAAAGTTAAAGCTTAAAGAAGTTAAAAAGTCTAGACTTGCAGAAGCCAAGAACAAATTAAAAGCAATTATTGATTCAGAATCTTCTAAATATGTTGATAGTTCTAGAGAATTGCAAATATTTAATGATTCAAATGTTGAAGAAAAATCAACACTTGAAGATATTGATAAAAAAATTGATGCTGCAATAAGAGCAAACAATGATTTAAATGAAAAAATTCAAAATTACAAATCAGCAAGATTATCTGATCTTGAATCTTCAAGAAATGATTTGTCAAACTTTATTGCAAACGATTTAAAAGATCCTGTATATAAAGAAATTGCAAATAAAATTAGTGAAAAGATTAAAGAAGTAAAAGGCGTTGGAAAAAATTCAACAATCAAAAGAATTGCAAGCAGCCATAAAAACTTTAGCAGATGCTAAAGAAAAAGCAATTAAAGAAAAAGCTTGGATAGATAGAGTTGGTATTAAAAAAGTTTTAGAAATTCAAAGATCTAAAATATTGTTAGTTGATGCTGAATATATTGCAAGATTTTTAAGATCAGGAAATGGTGAACGTTTTAGACAAGAGAATGAATTTAATAAATATTCCGGAACAGAATTTTTAAAATTAAAAAATGAGTTGGAAAGGGTTTCAAATCTTAAAGATTTAAATGCTCTTGACAGTGTTGATTATAAAGAAATATGAAAAGCTAATGAAGATTTAAAAAAATATATTCCAATAATTAATGGTGCAATTAAACAATTTGTATATGACAATTTTAAAAATTGGTCTGATGTGTTTTCTATTAACAATTTAAACAGTTGAGCTGAATACTGCTATCCGCCAAAATATACTGGTGCAGGTGAAAAATTAGTAGCATTTAAAAATGAAGTTCAACAAAAAAATGAAGAACTAAGGGCCAAGGCAAAAGGTGCCAGCTGAATGGATATTTTAACAATTTATAATCCACAATCATGAAAGCTTAACACTGCTGACATTCGTGATAAATTTAGAGAAGTTTATATAACTGAAGAAGCAAAGAAAAATAATCTTGCATATCAAGCTGTTGATTTATTAAACAAATTAGAATCATTTGATTTGAACAATAGCAAATTAAATAAACAAGAAATTAAGAATTTTAAAGAAAAATTAATTGCAAAAAATCTAGAAATTGAAGAATATTTAAGTAGCGAAGAAACCTTTAAAGATTATCGTATATATAACCCTAGTCTATGAATGCGTGATTTGGATTGAAAAATAAAAGAACAAGAAAAAACTTTAAAAGATATAAATTCTAAGATTTCTTCAATTGAAGATAGTTCATTTACAAATCAAGAATTAAAGAATACTTTTGTTGAATTAAAACAAAAATTATCAATATTGAATAAAAACTAAAGGGTTAGAGTCCCTTTAGGGTTAAAAAAAGTATATTAAAAATAAGGCTTCATAAGTGCCTTATTTTTAATTAGTCTGGATTATTGAACATCTTTTAGTTCGTTTAAAATATCATCTAATTCGTGGATATATTGAGATGCGAAAATTTCTTTTTCATAAAACTTAGGAAATTTTTTAATAGGAATAATGTTATCTTCATTATCTTCTTTAACTTTATAAAATTTTCAATTATAGTAGATATTTTCTCAATTTGTTTCACCTGAAAATATGAGTCTATAAGAAATTTTTTTGAAAGCAATTTTTGTAATTTTTAGTTTAATAAAAATTTTTATTTACTTATTTTTTAGTAATTCCTTTTGTTTTTGACCTATTATGTGGCACTTTCTGCATCTTGCTTCATATTCATTATAGTCACCAATTAAGTTTTGTTCATTTGAAGAATCTTTTCTAAAACTCGTTGAAGCAGCATGTTGACATACTACGCAAATTGCTTGTAATTTAGTTACTCGTTCAGCCATTGCCATTAAGGCAGGAATTGGCCCAAAAGGTTTTCTTAGATAATCTTGGTCTAGGCCAGCTACAATTACTAAATATCCCTTATTTGCTAAGTCATCAGCGACTTCAATTAATGAATTCCCAAAAAAATGGGCTTCGTCAATTATTATTGCCTTATATTTATCTTGCTTAAGTAATTCATAAACTTCATTGATGTTTTTTAATGAATGCGTTTTTCTTCTTACGCCACAACGGCTTACTATTTCATCATCAGAAAAGCGATTATCATATTCGGGCTTTATTAAAAGCGGTAGCATCTTAGCATATTCTAAGGTTCTAACTCTCCTTAATAATTCTTCACTTTTTCCACTAAACATTGGTCCAGTGATAACTTCTATCATTCCATCATTAAATGTTTTATACATTTTTATACCTCAAACTTTTAAATAATAATGTTTTAACATTATAATATAATTATTACTTTTTTAAAGGAGTTTTTATGAGTTTAAAAGCTGGTATAGTTGGATTGCCTAATGTTGGCAAAAGTACATTGTTTTCTGCATTAACATTAAATGAAGCTGAAATGGCAAATTATGCTTTTACTACAATTGAGCCTAACGTTGCAATAGTTAATTTAGAAGATCCAAGGCTTTATGAATTAGCTAAAATAGTTCAAACAGAAAAAATTGTTCCTGCTACTTTTCAATTTGTTGATATAGCTGGATTAGTTGCTGGTGCTTCAAAAGGAGAAGGCTTAGGCAATAAGTTTTTAGCAAATATTCGCGAAGTTGATGCAATTGTTCATGTTATAAGATGCTTCGAAAATGCTGATATAATGCATGTTAATAATAAAATTGATCCTATTAGTGATTTAAAAACTATTAATATTGAATTAATATTGGCAGATTTGCAAACTATTGAAAATGTAATTAATAGAATTGGCAAAAAAGCTCAAAATTCAGGTGATAAAGAATTGAAATTCGAATTTGAAGTTGCCTTAAAAATACGTAATGCATTAGAAAATGAAATTATGGTTAAAAATATGGATTTAAATCCAGAAGAACAAGCCATGATCAAATCATATCAATTGCTTACTGCAAAACCTACAATCTATGTTGCAAACCTTGATAGTGATAGTTTTAAAAATCTAGAAAGTGCTAAATATTATCAAGAATTAAACAAATATTTGCAAGACAAAAAAGAAATTTTAATACCTATTTGTATTAAATTAGAATATGAATTATGTAAATTTAATGATGAAGAAAAAGAAATGTTTTTAAATGAATATAACATTGAATGCTCCGGATTAAATTTAATTATCAAGAAAAGTTTTTATTTATTAAATCAAGCAACATATTTTACGGCAGGCAAAATTGAAGCAAGGGCATGAGTATTTAAAAAAGGAATGAATGCTGCTCAATGTGCAGGAATTATTCACACTGATTTTGAAAAGAAATTTGTAAAAGCAGAAGTCATTAAATATGAAGATTATGTTGCCTTAAATGGCGAAAAAGGTTGCAGGGAAGCTGGAAAAATAGCAATTGAGGGCAAGAATTATATTATGCAAGATGGTGATGTTTGCTTTTTTAGAGTGGCAAAATAAAAATTAAAATTAGGAAAATACCTAATTTTTTTTGCTGTATTTTTTTAAAATCGTATAATAAAGAAGTAATGATACTTATCATAATGATATGATATGAATTAACGAAATATAAAACTTGACCAAATTTATTTGCTATGGTTTTAAAGTTAATTGCATGATTAAAAAGTTTATTGATATACTATCAAAACTAATACTCATTAAAAGTTTCATTACATCATAAAAATTTTATTAGTCTATATATTGCTATATAGTATTTTTTGGTATTACATTAAGTTATTTGTAAGAAACGACTTTTAAATTTCAACCTATTTTGAGGAACAATTATGAATTTAATAAAAATTGATTTGCATGTACATTCTCAGGATTCAAGCCATACAGGAAGTAATATTTCCCAAGAATCGGATTTAGAAAAATTGTTAATTTTAACTAAAAAAAGAGTCAAAATTGCTAGTTTTTCTGACCATGATAATTTTTATATTGAAAGTTATTTGAAACGCCTTGAAATAATCAAACAATACAATATTGATATTACATTATGGCCTGGTATTGAAGTTAATTTAAGAAAATATGATGGTCAAATAGGACAAGCTATATTTATATTTGATCCAGATTGCAACTTAAAAGAATTAAATGAAGTAACTAAAAAGGAATTTAGATTCAATAGCAATAAATACACATACAAAGACGCTGTTGAACTATTTAAAAAAAGCAACTTCAATTTTATGGTTTTCCCTCATGCTGGAAAAGGTCAAGATAATATGGAATGGCAAGATATATGCAATAGCCAAGTTGATGGGCTGGATATAACAGATTTTAATAACAGCAACAAAAAGAAAATATTAAAACAAGATCCAAACATTCCAGTTTTATATTTTAGTGACACACATACATGAAAAAAATATCCTCAATATGGTAAATTTTGTTCTTATGTGGAGGTTGAAAATAAAGACCAAATTACATTTAATGAAGTAAAAAACAATATAAGAAATAATAAAGTTGATTATGACAAAGTATAGAAAGGAATAATATGATAAAAAGCCTAAAAATTAAAAATAATAACGAAACTTATGACATAAATTTAAATAATCAAGTTAATGTTATTGTTGGTGAAAAGGGTTCTGGTAAAAGCACCTTGCTTTTGATTTTAGCAGAAGCAATAGTAAACAAAAAATTATTCAAAAACGAATATGAATGATTCAATGAAAAGGCTAAATTTTTAGTCGATTCAATAAATATTGATAATACAGAAATTAAAACTCAGGATTTTTCATATATTTTTGATTCAGAAAATAAGCAAAATTCAAAAGATGCAGGAATTTTAGAAATTCAAAAAAACCTAAGCGGTTATATTAGCCAAAATGATAGCAGGAAAAATTCTCTTGATTCTTCTGAATATGTTGAAAAACAAAAAAGTAATTGTATTGATAATTTCACAGAAAAAATTTACTATGATCAAGATCAAAAAATGTTAAAACAATTAGATGATTTTAAATTGCTAGAAGATTTGTTAAATGAATATAGGCGCGAACGCGATAGACAGATAGCACTTTCAAATGTTTTTAATCAATCTTTTAATTTAAATGAAGGTAGTACTAAAAACATTGCAACCATTGACTATGATTCTTCAAATGAAATAAAAAAAATTAAGGAATTATTGAAATCAATTGAAGAAACAAGAGAAAAAATAAATAGTACAAATTTGGTTGTCAATAATCTTTTGGCACAATTTGAACAATCTTCGTCGCTACTTCAAATTTCAAATGAACAATTATTGGATATTCAACAAAGAACAGAAAATGTTATACTACAAAATAACTCATTTCTAGAATATTACAATCAATATAATTTAGTTGTATCCAAAGATAAAAAAACTCTTGAATGTTTTAAATTATCATTTACAAATGCTAAAAGAGCATATCTTGAAGAATTAAGCAACACTTTGAAGATTCAAAATGATAATGAACAATTATTGGAACATTTTTCTAATTTAGGAAGAATAATGAAAAAAATCAATAGACAATATAATTCAATAATTGAAAAAGAAATCATTTTAAACTGAACATTAGAAGAAACTGATAAAAAAAATAAGAATATAAAATATAAAATAGAAAGTTTTGCTTTAAGTGAAGAAGAAAAAAATTTAATTTTGAATAAATGACTCGGTTCAACAAATAAGAGCAATACAATCGCTGATGTTTTTAATGGTAATATTAAAGATAACTTTGATATTAAAAAGATGATTAAGGCATTGATTAAAGATAAAATAAAAATCTATGCAGGGGATGAAGAATATAAAAATCTATCAATGGGCCAAAAGACATTGTTTGGTATAACACACGCGATTGATACATTGAACAATGTTCCTAATGAACAATACTTATTGCTAGACCAAATTGAAGATAATCTAGACAATAAAACAATATATGAAAAAATAGTTCCAATGTTGCAAGAACAAATTAAAAAAGACAAACAAATTTTTATAGTTACACATAACCCTAATATTGGAACATTAATAAAAGGCAATATAATAACAACAGATATTTTCAATACTAATCTAAACAAAAAATTTGTTGTTAATGAAATAATTCGCGAAGATAATGAAATTGACACACCTCAATCGCTTTATTTGGAGGGCAGTATGAAAGCCTTATATGAAAGATTAAAAGTACATGAAGAAAAAATTAATAAAACTAAAGGAGATAAATAATGATAACAATTGACTATGTATTTACAAAAGATGAAAAGAGATTAATAGTTATTTCTAATGCAAGCGATAGCAAAAATAAATATAAAATTGAAATTGACTTAGACAACCCTTCTGATGCTTGAAATAAAGAAAACATTAATAATTTTATAATTAGAGCAATATCTATTAGTGATGAAAAATTAAGTGAACCACAATTAACGGAATTGGCTCAAGAACAATTACAAAAGGGTAATAAGCAAATAGAATTTATTAAAAATTTATTCACAAATTTTGTTGAAAGATATAACGAAAATTAATTTTTATTCAATTTTAAATAAAGCACAAGGTTTTTAATAAAAACATTTATTTTTAAGTTGAAAATTAAAAAACAAGGCCTTAAAGGCTTTGTTTTTTTTAAAGAATTTTACATTTTTTCAGGTGCTTCAATGTCCATTAATTTTAAACCATCAGCTAAAACAATTTTTGTTGCCTTTACTAGGGCTGCAAGGCTTTGTTCGTCAGGAGATCCTAAAACTTTGGTATTTGAATAAAATGAGTTAAATGCTTTTGATAATTTTAATAGATAAGGAGGCAACAAGTTTACTTTATAAGTTTTGGCCATTGTTGAAATTAATTCAGGGTATTCTTTTAATTCTGCAACAAGCTTTTGAGCATATTCGTTATCCAACTCATCAGAAGCAATAGGGTTTTTAATTCCTACTTTATCCAATAATTGGCAAGCACGAGCATGAGCATATTTAATTATATACATAGGGTTATTTTCGTTGTTGTCAGTAACTTTGTTAATATCAAAGTCAATTCTTGAATTATATGATCTATCAATTAGAAAGTATCTAATTGCATCATTTCCAACTTCTTCTGCTAGTTCTCTTAACGTTAAAGAGGTTCCTAAACGTTTTGACATTTTAACTTCTTTACCATTCTTCATAATTCTAATTAGTTGGAATAGAAGAATTTCAATTTGGTCTCTTCTATGTCCTAAATCTTCAAATGAGAATTTAACACGTTCTACGTATCCTGAGTGGTCAGCTCCTCAAATGTCAACAATTTTTCCATTTGGATCATTTAATTCCAAAAGTTTTTGTTCGTGGTATGCAATGTCCGCACAGAAGTATGTTGAATCACCATTGCTTTTGATAATAACACGGTCCTTATCATCTTTGCCACCCTTTGTTGTTTCAAGTCAAGTTGCGCCTTCTTTTTTGTATGTTGTCTTTAATCTGTGAATTGCTGGTCATACTCTATTTTTGGCATATTGTTCTGCTTCTGATGTGAAAATATCGAATGTTACTCCAAATTTTTTCATATCTTCAATAATATTTTTTAAAGCAAAATCTCTTCCAAAACTTCTAAAGATTTTTCTAATTTCATCAGTATATTCTACATTTTTGTATTCATCTTTGTGAATGTCATAAAATGCTTGAGCGAATTCTATCATATCTCCGCCATGGTATGAATCTTCTGGCATAGGAAATTTGGGATTAAATATTTGTTGGTATCTAGCAAATATTGAATCTGCCATTTTATTCATTTGGTTTCCATAATCATTAACTAAATATTCTCGAACAACGCGGTGACCTGCTTTTTCTAGAATATTGCACAATGTTGCACCAATAGCAGCATTTCTTGCGTGTCCAATATGCAAATATCCTGTTGGGTTAGCAGACACAAATTCAACATTAATTGCTCCTTTACCTTTTGGATCAATATTTCCATATGTTGATCCTTCGTTGTTGATTTGATTTATTGTGTTGGCTATAACTGAGTTTGAAACTCAAAAATTAATAAAACCAGGTTCGGCAACCTTTATTTCACTAATTTCTTTAAATTTATTTAAATCAATATTTCTTACTATTTCTTGGGCAATATCTATTGCCTTCTTACCTAAATCCTTTTGCAATGTTAAGGCAATATTGCTTGAATAGTCGCCGTAATTTTTAGATTCAGTAAGTACAATAGGCTTAGAAATATTTAATTTTTCAAGTGCTTCATTTAATGAAGCAATAATAATGTCTTTCGTTGTTTTCATATTCATAATTATAATATAATAAAATTTAAAAACATTATATTTATAGTTATAATATTGAGTATGAAAAGATATTATTTATGTGGCCCAACTGTTTATAATTATCCCCATATAGGCAATTTAAGGCCAATAGTTAGTTTTGATTTAATGATTAGGGCTCAAAGGCAATTGGGAGAAGATGTTTATTTTCTTCACAATATCACTGATATAGATGACAAAATCATTGCTAAGGCAAAACAAGAAAACAAGACAGAAAAAGAAATTTCAAGTTTTTATGAAGCATATTATTTAAATTTATTTAAATTATTCTCAATTGAAATGCCAAATAAGATTGTAAGAGTTACTGATTCCCTTAATGATATGTATGAATATATTCAATCATTGATTGAAAAGGGCGCAGCATATCAAGTGAACGGCAATGTCTATTTTGACGTTTTGAAATTTAAAGATGTGTATGGAAAAATAAGCAATCAAAAATTGGCAAATTTAATTTCAGATTCAAGCAATGAAGTTGACAAGCGCTCACCATATGATTTTGCTTTATGAAAAGACACAACTGAAGGTATAAAATTTCAAAGTCCATTTGGTCTTGGAAGACCAGGTTGACATACTGAATGTAGTTGTTTTATAAATAAATATTTTGAAGGCAAACAACTTGACATTCATGGAGGAGGTATTGATTTAATATTTCCTCATCATGAAAATGAAAACATTCAACATTTTGCAATCAATGGCCAAGATATTGCCAAGGATTGGATCCACTTTGGTACATTGAATTATAAGAATCAAAAGATGTCTAAGTCAATAGGAAACATAATTTATGCTGAAGAATTTTTAAAAAATTATTCAGCTGATACTTATAAATTGTTAATGCTATCAACAAGTTACAGCAAACCAATTAATATTACTGATGAATTACTTGCATCGCTTCAAAAACAAATTAATAATATTGAGGTGCATTTTAATAAAAGTCAATTGGAAGATATTAAATATACGCTAAACAAACAAACAATAGATTCAATTTTAAAAAATGTTTCTAATCTTGATTTTTCATTAGCCTTTAAGCAACTTAATGAGCAATTAAAATCACCATTGAATTATGGTAATGCGTTTTTTGTATTTAAGGTTTTAGGCTTTAAATTAGTTGAAAATAAAGTAAGCAATGATGATAAAGAACTATATAGAAAGTGAAATATTCTAAAAGAACAAAAGCAATACGATAAGGCAGATGAAATAAGAAACATTTTAAAAACAAAAAGGTTGATTTAATGAAAAAGATAGTTTTTGGAAAAAATTCAGTCTTAGAAGCAATTGAATATGAATTTCCTATAGAAGAGATTTACTTGCAAAAAGGACTTGTTAACAATAATTTAAAATTTAAGAAAGTTGTTTATTTAGATAGAAATAGCCTAGATAAATTAACAGAAAATGGAAATCACCAAGGATATGCTGCAAAATTAAAAGATTTTCAATATCATGATTTGGGTAGCATTTTTAAAGATGGCTCAGATAAGGTTTTAATTTTAGACCATATCCAAGACCCCCAAAATTTTGGTGCTATTATCCGTAGTGCCAATGTGTTTGGCTTTAAACATATAATCATTCCAAAGGATAGAGCATGTGATGTAACTCCGAGTGTTTTAAAAACTAGCTCAGGAGGTTTTAAAAACGTTAAAGTAATTAAAGTTAACAGCTTATTTGATGCAATTGAGGAATTAAAAAATAAAGGTTTTTGAATATATGCATCGGCCTTAAACCAACAAGCAAAAAAACTAAATGACGCAAAATTTAATGAGAAAATTGCAATTATTGTCGGAAACGAAGGGAGTGGTGTTTCAAAAACATTGCTTAAACATTCGGATGAAATTATTTACATTGAACAAGATAAGGAATCTGTTCAATCTCTTAATGTTTCAGTGGCAACAGGAATTTTGTTATATACAGTTTTTAATAAGAAATAAAAAAATAACAATTTAAAAAAACAGCGGTTCTCGCTGTTTTTTTGTTTATATAGTTTTTTAATATACGCTTAATTTAAAGTAACTTTTGTACCGATTTTTTCTGCTAGAATGTTTTCAAGATCTTTTAAATCACCAATAAATGCTGCTTTTCCGCCTTCTTCAACGAATTTAATAGCTGCTTGTACCTTTGGTAACATTGATCCTGGGGCAAATTGTTTTTCAGCAATGTATTTTTCAAGTTCTGCTTTTGTAGCGTGTTTTAGATCTTTTTGATCTGGCTTGTTGTAATTTACTTTAACATAATCAACTGCTGTTAGAACAATAAAGTAATCAGCTTTTGCTAATGCAGCCATTTTTGCTAAAGCGAAATCTTTGTCAATAACACCATCTACACCTTTAATAAAGCCATTTTCATCTTCAACAGTAGGAATTCCACCACCACCACCAACGATAACGGTAGCACCTGATTCAATAGCTTTTTTAATTTGATTAATACCAACAAAGTTTAATGGTTTTGGAGATGCTACAACTTTTCTGTAGCCTCTACCTGCATCTTCAACAATTGTGCTATTTGGGTTTGCAGCAACTGCTTCTGCTTCTGTTGCAAAGAATGGGCCAATTGGTTTTGTAGGATTTTGGAATGCAGGGTCTTTTGGATCTACAAGAGTTTGAGTTAAAATGTATAAAACTTCCTTGTTTTTTAATCCTTCGTTTTTAAATGCATTTGTAATAGCATTAACCATATGATATCCAATGTATCCTTGGCTCATTGCACCTGCTTCAGGCAATGGAACTAAAGGAGACTTAGGATTAACTGAGTGAGCACTAACGAAACCATTGAAAATCATTCCAACTTGTGGTCCGTTTCCGTGTCCAACAATAACTTCGTGTCCTGCTTTTACTAATTCAGCAATTTTGGCTGCAGGAACTTTGACAAGTTCTTTTTGTTCTTCGGGGTTATTTCCTAAAGCGTTTCCGCCTAATGCAATAACAATTCTACTCATATTAGTATCCTAAAGTAGCTAGAATAACAGCCTTAATTGTGTGCATACGGTTTCCAGCTTGTTCGATTGATTTGTTGTATTTTGATTGGAATACTTCATCAGTAACTTCCATAGCACCGGTTGAAACAACTGGGTATTTTGCGCCTAAGGTTTCTTTAATAGTTGCTGAGAATTGAGTTTGGTCATCGTGGAATGCTGGTAAGCAGTGTAGGAAGATAACATTTTCTTTTGCTGCTTTGATCATTGCCATATCAACTTGGAATGATTGTAATTCTTGAATACGTGGTTCAAATAGTGAGAAGTCTTCTCCTAATGATACTCATACGTCTGTGTAAATAGCATCTGCGTTTTTAGCAGCTTCTAATTTGTTATCTGAAAATGATACAGAACCACCATTACGTTTGAATAGTTCTTGACATGCATCGTAAACAGCCTTGTGGTTTGGTCCATTTTTAACAATTTCTCATTGAGCTTTTGGTCCACATAATACAATATTAACACCAAAGAATGCAGCACCAATCATTAATGAACGAGCAACGTTGTTTTTAATATCACCTGAGAAAACAATTTTCTTTCCTCTTAGGTCTCCAACGTGTTCTTTAAATGTTAAGTAGTCAGCTAGCATTTGTGTTGGGTGTTCAGCATCGGTTAAACCATTTCATACAGGAATTCCTGAGTATTTTACTAATGCATCAACATCTGATTGTTTGAATCCACGGAATTCAATACCATCATAAATTTGGCCTAAAACTTTAGCTGTGTCAATAACTGATTCTTTTTTACCAAAGTTTGATCCTGTTGGGCCAATGTATGTACAGCTTGCACCTAAATCAGCAGCTGCAACTTCAAATGAACAACGAGTTCTTGTTGAGTCTTTTTGGAACATTATAACAATGTTTTTTCCAACTAATGGGCGATTATTTGTGTGTAAACCAGCAAATTTTGCTCTTTTTAGTTCTAGTGATAAATCTAGTAAATAGTTAATTTCTTCGGTTGTAAAGTTTAGAGCGCTGTCTAAACTACGACCCTTTAAATTTAATGGCATATTTTCTCCTTAATTAATAAAATGATTTATTTAATAAAAAATAAGTGTGTGTTTTGAATTTTTCAAGTTTTTTAAAACACGTTATAATTATACAATGTTTTAAAAATATAGATTTTTTATTTTTTATTATAATTTTTATAAAAAAAGATAAGATTAATTTTCAATAACCTTATCTTTTACTTCTTTAAAAAATGTTTTATTTTAAATCATTTTTTAAATCTTTTTTATTCCCAGAATTTTTAGTTTTTAATAAATTTTCATTTAATTTTTTTAATTCATTTAAAATTAATTCTTCTGTTGTTGGAGTAGGTTCTTCCTTTATTTCTTCTTCAATTGGGTTCTTTTTGGCTTTTGCATATAGAATAGAATTCTTAATTGTAAACACAATCATTAATCCTATAAAAATAAATGTTGAAACAATAACAAATTGAATTAATGCACCCAAAAATTTACCAATAAAAATTCCACCAGTTTCTTTTGTTGCGTGAATGCCTGGTCAAACCACTCAAGCATCAAGATTTTTCATACCGACTGCTTTTGTTATTGCTGACATAATAACATCATTTGCTAGTGATGATACTAGTGCCCCAAATGAAGCTCCTAATAAAAGCCCAATTGCTAGCATAAACATGTTTCCACGCGAAACTATTTTTTTTGCATCTATATATGACTTTTTAATATAATGCTTTTTTTCTTCTAATTCTTTACTTGTCATATCAATATTATATAGAACAAAAATAATTTATTTCTTGTTTTCTAACTAACAATAAAAAAAATAATAAAAAAATGGCGGGTAAGAGAGGATTTGAACCTCCGCGGGTGTTTTAGCACCCCTACAGTCTTAGCAGGACCGCCTCTTCGACCACTTGAGTACTTACCCATTTGCGCCATTGCATTAAAATTATATATAAATAATTTAAAAAAATAATAAAAATAAATCAAGGAAATTAATTTTTCCTTGATCTTGGAGGGTAAATTTTATTGATATCATTGCCACTATCAAAAATTCCTAATTTTAAATTTTTAGCATGATTAGAAGCTTCTTGCAATTGCTTGTAGTAGTCAATGTTCTTTGTATAATAATTGCTTTGAGGATCTATGCTAATATAATACATTCTTGCCATTCCAAAGAAAACCAATTGTTCATTTAAATTATAAATTTTTTTAGTTATTTTGTCTTGATAGTAAATAATTGCAACAATTCTTTTATAGTTATCATAAAAATTTTTGTCTTTTGTATTAACCCTTGATTTAGTTTCCTGTGGGACTACTCAAATTTTAGCAGCATTGTTTAGAAAATTCTTTGTATAATTTTCGGCAATTTTTCCATATTTATATTGAATGCCGGATGTTTCTTCAAATTTGCCATTACTCTTTTTATGAGTTTCCGGCGTATCAATTCCATTAAAGCGATATTTGTTTCGATAAGAATCTGATATTGTATCTCCATCAGCAATCCTTGCAATATTTATTTCTGTTGCTTTTCCTACTTCAAATTTATTATTTTTTAGTATTTCAAAATCAAATTTAGCTTCATGAAAATTATTGATGTTAAATTTGTCATTATTTGTGTCGTTTTGATTATTTGGCTTAGCTATCTTAGTAAGTTTTTTTATTCTTGATAACAAAATATCAAATTCTTTAATTCAATTAACTTCTTTGCTATTTGAATTTATTAATTTTTTGGCATCAGCAATTAATTGTTTATAATAATCATTTTTTTGCCATTCATTGCTATTTTCAATTTCCTTTATTTTTTTGTCATATTTTTCTTTAAAATTTTGTTGTTCCTTGTCATTAATTTTTTCTAGCTTACAAGAATATGAAATTAATGAAATTGGCAAAATTGTAGCAATAGAGCCCAATGTGAGATTCAATATTTTTTTAGTATTTCTTTCCATATTTATTCAATAAGTCCAACGTACATTTATATATTGCTTGATTTGAATTTGCATAAAGAATTCTTGGATTGTAATTTTTGTTTTCTAAAAAATTGTTATTTTTTAAAAAATCATTTAGGGCTTTTGCATTGGCAATTTGCAACTCGGTATTGAAATTTATTTTTTTAATGCCCAATCTAATTGCTTTTTTAATGTCATCTTCCTTGATTCCACTTGCTCCATGTAAAACTAATGGAATTTTTATTTCTTGTTTTAATTTGTCTAATAGTTCAAAATCTAATCCCTTTCAGTTACTTTCATATTGACCATGAATATTTCCAATTGCCACTGCTAATAAATCAATTCCGGTATTTGCTAATTTTTTAGCATCTTCAATATTTGTTTTTTGAATATCAAGTGATTTATTTTTACTAATATCTAGTCCGATCCTACCTACTTCTGCTTCAACAGTTATATCCATTTTTTTTGCAATGTTAACTATTTTTTTTGTGTTCAATATATTGGTTTTAAAATCTTCTTTACTACCATCATACATTATTGACTTAAAGCCGGCATTTATAGCATTAATACAATCGTTATAATTACCATGATCTAAGTGCAATATAACATTAGTTTTAATTTCTAAATCTTGAATAAGACCGTTAACTAAGTTGACAACGGTTTTATATCCGCCCATGTATTTAATTGCAGAAGGAGAAGCTCCAAGTATGATATCTAAATTTGCCTCCTTTGTTGCCAATAATACGAATTTCGCTCATTCTAAATTGTTTATATTTATATGCAAGATTGCATAATTTTCATTTTGTATTTTTTTAAGTGTTTGATTTAAATTTTGTATTTCCATATTTTTTCTCTTTAATTTTATCATTGCAATAGTCATTTTAAAATAATAAAAGAAATAAAAAAACTAGCATACACTAGTTTTTAAATTCGGGTCTTTTAGAAGTTCAAAGTCCATTTACTTTTTTAAAAAAACGCCCATCTATTGTTAGTAGTTTATATAATTCACCACGTTTTTTTATTAATAGCTCTTCATCAGAAATACTAGATGTTAATTCTTCTTTTCAACGTTCAAGTAGTTCGTCTTTTACATAATTAAATAAAGTTTCAAAATCTAGTGCATCATGTCTATCTAAAATTTCTTTAGCTATATCAACTTGCGATTTATATTCCATAATCACCTCATAAAATTTGTTTCTTATTATAACACTTTCTTACAATATATCTAATAAGCAATTCTCTATATATTATATAGATATAACTTTCATAATGACAAAAAATGTATATAATGGCAAAATTTTAGTTAACTATAGTTAGATTTTTTTAAGTAAGTATATTGTTTTTTACTATAGACCATGAAAATATTTTGAATTTTGTTTTAAATTCAAAATATGGAAATAAATAATTGTTTTATGTAGAATCTTTATATATTTTAACTTTTTTAACATTAGCCTGCACTAATTTTAAAATATGATGCATTTTATGCATAAAAAATGTTTTAAATTCTTTGCAAATTTATAAACAAAAAAAATATAGTATAATTTATTACGTATAAATCATAATTAATGCAATAAAAGTGCATTTGTATTTATACGCTATAACATCTATATTTGAAAGGAATAAAAAATGTCTGTATTTGACAGTAAATTTAATGGCATTCACGTTTATTCAGAAATCGGAGAACTAGAAACTGTTCTAGTTCACGAACCTGGACGTGAAATTGACTACATTACACCAGCTCGTTTAGACGAATTACTATTCTCAGCTATTCTAGAAAGCCACGATGCTAGAAAAGAACACCAATCATTTGTTAAAATAATGAAGGACAGAGGAATCAACGTTGTTGAATTAACTGACCTAGTTGCTGAAACATATGATTTAGCATCAAAAGCAGCTAAAGAAGAATTCATTGAAACATTCTTGGAAGAAACAGTTCCTGTTTTAACAGAAGCAAACAAAAAAGCTGTAAGAGCATTCTTATTAAGCAAACCAACTCATGAAATGGTTGAATTCATGATGTCAGGTATTACTAAATATGAATTAGGAGTTGAATCAGAAAACGAACTAATCGTTGACCCAATGCCTAACTTATACTTTACACGTGACCCATTTGCATCAGTAGGTAATGGTGTTACAATTCACTTTATGCGTTACATCGTAAGACGCCGTGAAACACTATTTGCAAGATTTGTATTTAGAAATCACCCTAAACTAGTTAAAACTCCATGATACTACGACCCAGCTATGAAAATGTCAATTGAAGGTGGTGACGTATTTATCTACAACAACGAAACATTAGTTGTTGGTGTTTCAGAAAGAACTGACCTAGATACTATTACTTTATTAGCTAAAAACATCAAGGCAAACAAAGACGTTGAATTTAAACGTATTGTTGCAATCAATGTTCCTAAATGAACAAACTTAATGCACCTAGATACATGACTAACAATGCTAGACAAGAATAAATTCTTGTACTCACCAATTGCTAACGATGTATTCAAATTCTGAGACTACGATTTAGTTAATGGTGGTGCAGAACCTCAACCACAACTAAATGGATTGCCACTAGACAAATTATTAGCTTCAATCATTAACAAAGAACCAGTTTTAATTCCTATTGGTGGTGCTGGAGCAACTGAAATGGAAATTGCTCGTGAAACAAACTTTGACGGAACCAACTACCTAGCAATTAAACCAGGACTTGTTATTGGTTATGACCGTAACGAAAAAACAAATGCTGCTTTAAAAGCTGCTGGTATTACTGTATTGCCATTCCACGGAAACCAACTATCTCTAGGTATGGGAAATGCACGTTGCATGTCAATGCCTCTATCACGTAAGGATGTTAAGTGATAATTTATAAATATTTATAATAATATTTAAATTTATATAAATAATAAACGGACTCACAATAGTGGTCCGTTTATTTTATATGCTTTAAATAAAGATTTGTTTAGATTTGGTTAGTTTTAATAATTATTTTTTATTCTTTCATAGTTTTTATGATTTTTTTTGGCATAGGCCGTACAAATCATATCGCGATCAATATTCATAATTTCACCTAATCCCAAATAAACACCATATGCTTTTTTTATTAATCTTTTATTATGGTGCAAAAACAATTTGGTAAAGCATTGATATGCATAAGCAAGTTGCAAGGTAAAGTCATTATATTTAATTATTGGCTTTATATCAAATGAGGTTATGTTATTTTTTAATGCGATACTAGTAATAAAATGAATTCCATCCGCATATTCTTCTAACATTGAGATTTGGTTTATACTCTTATCTTTTTTTCAATATTTAAATAGTTTTACTTCGTTTGCAAATTCTCCTAGTTCAACAAGCAAAGCAATAACCATTTTGATTTTGATATTCAAAAATTCTGTTTTTTCAATACTATTTGCAAATGCAAGGTCTAATTTCTTTTGGGCTTCAAAAACTTCTGTTAAATTCATGTTTTGAATTATAAACTTAAAAGTTAAAAATAATTCCATAATAAATTATTTTTTTTATTATAATTATTTGTATGCCCCGATGGCGGAATGGTAGACGCAGTTGACTCAAAATCAACCGGTGAGAGCCATGCTGGTTCAAGTCCAGTTCGGGGCACCATATCTTTTAAGACTATTTAAACTAGCAGCAATGCTAGTTTTTTTTGTATTTTTTATATCTAAAAAAAGAATTTGCTAGTTATAACTAGGCATTTTACTAAAAAATAAATTTATCAATGTTATACTTAAATAGTTAATTATAAAAATATGGGGAATTAAAAGTATGGATAATAGGAAGCAAGAATTAAAACAGTTAAAAAAAATACCGCTACAAGAAAATTGACCTAGAATTTTAATATTGCTAATATCTGCAATTGGTTTACTTGTTTTAATTTATATTACATTGGTCTTTAAAAACAAATATGAAGAATCTCTTGAACTTGAAATTTTTAATGATATTTTTGTAAGTTGTTTAGTGGGAATATTTTCAGGAGCATTATTAGTCTTGGCTTCTTTTATATTATTAGATGTTTTTAAAAGAAATAAAATCAAAAATTTCTTTGAATATTATTGTTATCTTTCTTCATTAAAAAGTAAAAATAAATTTTTATTTTTTAAAAATTATGATTTAAGAGAAATAATAAATAATAAAAATCAACTAACCAAACAAGAATTTATTTGCTTTGTTGCCGAAAAATTAAACTATACAATGCCTTCTATTGAATATAAAAACTTAGTTAATGAAATTAATAGCGATTTTGCCAAACATTCATTTTTAGATCCAAATTTTAAAAAGCAAAAGAAAGCAACACTTACTAGGTTAATTATCTTTGAAATTGTCATTCCTTTATTTATTTCTATTGCATTAATAGTATTAATTGCATGTCTTTTTAACAATGATGATCAACCAATCAGGGCAATTTATAGATTGATAATCATTTTAGTCAACGCAATAATTGTTTTAAGCCTATCGATTTTAATTTATGAACTATTTATTTTAAGTAAAGTAAAAAATTATCAAAATTTCAATAATTTATATTTGCTTTCATTTAATAATTACTCATATAAGTATTTAAATTCTAGTTTAGTAAGATAGCAAAAGTACAATAAATTACAAAAATGTTAATTTATTTGAAATACTTTACCTAAAAGTTTTATAATTATACGTTTTAATAGGACTTATAAACGGGCAATTTATGGTTTTATTAGTAATAATTAATTTTTGTAGCAATTTAGATCTAAATTGTTAATTTTTTGTCTCAATATTAATTTAAATATATTAAAATTATCTTTGTGGAAACACAAGATTTATTTAACGCTATTAAATTTTATAAATTAATCTCAACCCTAGTAACAAGCTAGTTATTTTTTGGTTGAGATTTTTTAATTTATTTTAATTGACAAATATTAAATTTAAGAACATTTTTAATTACTAAATTGAAAGGAATTATAAAAATATGAAAAAGATAAACAAAATTCTTTTAGGGCTAGCACCTGCTGCAGCACTTATTGCTGCATCAGGACTTTCTGCTGCTTGTGGTCATACAGGAACCGGCTATGGATTTGACCAAACAGATGATGGAAAATTAGTATTAGCCTCAGGATTTTCTAACACAAATAAACAAGGTATTGCCTTAAAAGCAGTTGTTGATGCATACAACAAATGAATCAAAGACAATAATAAGCAATCAGAAGGATATTTACCTGTTGAAGTTAAAACATTGGCTAATGGATATAATACTAACACTTTAAGAAGTGACTTGGATGCTAAAAACTCAAATGACTTTTATAACATGACATTAAATTATCCAGCTGCTGCTTCGATATTGTCTCAATACAAAATGAATTTAGCTATTTCAAAAGAAGATTATGATAAATTCGGTATTGAAAAAGGATTTAATTTGGGTAATGATTTAATTGGTGGAAACACTAAAAAAGAAAAATGAGTAGTTCCTTTATCACGTTCATCAGAAATGCTTTCAGTTGCTAAGGCATTAGTAGGAAAATTCATTAAAGAATTAAAAGACTTGGGTGTAAAAATTTCTGACAAAGACAATACTAAAGTTAACGAATATCTAAAATATTACACAGAAAATACAAATGAAGCAAAAGGTGTTGATGAAACTTGAGCACATGCTAAAACAGAAAAATTAGAAGAAATTAAAGAACAAATAGTTAAAGCATTGCCAGAAATGTCAGATCAAATCTTTAGAGATTATTCAAGTATGATTAATTTCTCAATTCAAGCAAAGAAAATGTACAACAAAGATCCTTTATTAAATGTTATTGGTTTTGACTCACTACCAAATGTTATTAATACAATGACAACTTCATTAACCAATGGAGATATTAATAAAGGATATATAAAACCAGATGAAGATAAAATAACAACAGGTGGATTTGACTACTCAACATTTCTAAAAGATGCAAATAGTGAACAATCAAAATTATTCAAAAGGATAGTTGACTTAATCCTAGATGGTGTTAAGGAAGGCGCTGTTTGAATTGGTGGTGCAGGTGCTTATGGTTCAAATAACTTACTAAAATATCAATTAGCAATGAGCCTTGGATCAACCGCCGGATATTCACATACATTCTCAAACGCTCAATCATTAACAAACTTAAAAATAAAAGGCACAACTACTTCATTAGATCAATCTACATTGGAAGTTTTAGGCCAAAAACCTAAAGAATCTGACAAATGAGTTATTCAAATTAAATCAGGCACACATAAAAATAGTGTATACGGCAAGGATAATTGAGCAAAAGCTTCAAAATATGATAAGAAAATAACTCAAGAAGCAGCTAATATAATTAAAGAAAAAGTCCAAGGTCAAGAAGGATATTTAGTAGAATTATCTTCAACATTTGAATATAAAAATAATAAGATTTCTCTAAAGAAGGGCAATAAAGAATTAGATGCTGTTTATTTAGGAAAAATTTATGAAGGAAATGAAAAAGAATATTTCTTCTTAAAGAAATCGCAAGTTGAAAATGAAGTAGTTTCATCAAATAAAATTTTAAATAAAGAAGATGCTGACTGAATTTCATCACCATTGGCATTTGATAAAAATGCTAAAAAAGCAGTATTTATTCAAGGACCTTCAATTCTTCCAATTCACGCTAATGAAAAGGAAGACAAGGCAACTAAATTATTCATCAACTGAATGTTCCAACACGATTTAGAAAACTTTGAAATTGAAGAAGTATTTAAAGGAAAAACTACAACCACAAAATTCGACGGCAAGCAAAAACCTATTGACATTTTCAATGAATACGGAAGCTATATTTCACCAACTAAGTCATACTTCTCATCAGATAAAGCAAGCAAATTAAATACAGCAACAAAAATAGCATTTGACAACTTTAAACAACTTGACAATAAAGATAGTGGTTATATCCCATCAGAAGATATTGCAACTTCATTATCAGATGTTTTAAGAGAAGCAATAACAAGTGCTGGAAAAGCAGCGTTATCAAAAGCAAACGTTAACGAAGTATTTGAATATAAGGAATTTGTTTCAAAAATTTTAGGTCAATTAAGTAACCAACTTTAATAGATTTTTATTAAATATTAATATTTAAAAAACATAAAAACAACCAAATTTGGTTGTTTTTATACGGAAATTAGAGAAAAATATAAAATGAAAAAATACAATAAATTTTTATTAATTTTAGCCTCATCTGCTGGCTTAGTGGTCGGGGGTCCGCTACTATCTGTATCATGCAAGACAGTGGATAAAGAAAAAATAAATTTATCAAAATTTGATGTAATAAAGAAAAATTATGAAATTCCAGTATATGAATTTTCAAAAGTGGGAACAATTTTTAATAAATCAATAAATTATAGAATGTATGTCACTGATGAAAAAGGAAATAAAACTATTAGAGAAGTTTCTCAATCATTATGGGATTATTATAATTACATTGAAGGCGAAGTTACTAGAATTTTTGATGGCGACACCATTGAAATAAAAGTAACAAAGCAACCAAATCCTAAACCTGGTGGTAAAGTTCTTAGTGTTCCTACAACAATGAGATTAAGAATACCAATGATTGATACATTGGAAGAAAATACGCCAGAAGTTGGAAAAGAAGAAAAGGAATTAGCAAAAAAAGATCATGCATATGCCGAAAAATTAATTCCTATTGGTTCAAAAGTTAGAGTAATTTCAAGCAATTGAAGTGAAAAAACATATGATAGATTTGTTGGATATGTCTTCTTTGGCAAAAATTTTGAAAGACAATTTGATATTGAAATGTTAGCAGGTGGATATACTCTTGCTAGATTAGATACAACAAAAGTTATTTCAGAATTTATTGCTGATTTATCAAAAGAAAGCACTGAAGCAAAGGAAATAAGAAGTTACTTGTTGCCTTATGCTGCATATGCAATTAATTATGGAATTTTAAATAGAAAAGGATTTTATGGTGAAGTTGCCAACATGCGTTCTCCATATGAACTAAGTTCAAAATATTTGGAACACGGACAAGGTATGGTTGATTTCTCACTTTCAATACTTCATTATAACTATTGAAAGAAGCCCAAATTGGCAACTAAAAAGAATAATATCTATAGATATTTAGAACAAATTAATCAAAATAAAAAATAAAAGGAAATTCTATGAAAGAAACAAAATCAAGAGAAGAATTATTAAAAGATATTCATAGTTTTTCTAAGCAACGTCATGAAATTAAAAAGACATTGCCTGCAATTGAAATTAAAAATTTAGTTATTGACTTTGGTGAATCACTAGCAGTCGATAATGCTTCATTTTGCATTAATAAAGGAGAATTAGTAACATTATTAGGACCATCTGGTTCTGGTAAAACGACTACCTTGAATGCCATTGCGGGACTTTTAAGACCAACAGGAGGAAAAATTTTCTTTTCAGGAATTGATGTAACAAAATTCTCACCTCAACAAAGAGAATTAGGATTGGTGTTTCAAAACTATGCACTATATCCTCACATGTCAGTTTATGAAAATATTGCATTTCCTCTATACAATGACAAACATTGACGTGAAGAGACTCTAGAAAAAACTAAATTATCACAATTGAAAATTTTTGAAATCATTTTAAATGCTTATAAAGTTGATGAAAAAGCAATTGAAGATTTAAAGACAAAATGCTACTATTCAATTGACAACCCTAAAGAAACTAAAAAGTATTTAAATGAATTAATTTCACAATATAATGATATTATTGATGAACAAATTAACAAATTAAACTTTTATAAAACTAAAAAAACAGCCGAAGGAACCTCACTAACTAAAAATGTTTTGCAATATATTAAAAAACTAGGATTGCAACGCCAAGCAGAAATTTCGGAAGCTAGAGAAAAATTTTTGGGTGAAAAACAAAAACTTGATAGAGAATTTGAATTTACTCATGACAAAAATATTTTGAAAAATATATCAGCATTAAAATCGGAATTTTCAGATACTGTTTCTAAAATTAAATCTAAAAGTGCAGAAGAGATTGCTAATGCTAAAAAAGAATATTCTGAAAAATATAACGAAGCGTTAGCTCGTAATACTCAAGCAATTAAAGATGCAAAACTAGAACTAATGGAAAAGAAACGTGAACAAGCAGAATCTCCTTTAGTTAATAAAATAAAAGAAATAAAGAATAGATACGTTTTAGTTTCAAAAATAACTAATGTTGAATATACAGAAGCAGTTGAAAATGTTTTTGGTTCGTTGTTCAATTCTCAAAATATAACAAAAGGTAATTTTGATACTAAACTTAAGGCTTTAATATTTGCTTTAGATCAAGAAACTCAAAATCAAATTTCAGAAATTTCTAAAGATGTTTTGACAATTGACGAAGCAATCGTAAAAGATGTATTAGATGTAGCACAAAGAGTTGAAATTACAAAGAACTTAGCAAAACGTCCTACTCAAATGTCTGGCGGTCAACAACAACGTGTTGCCATAGCTAGAGCCATCGTTAAAAAACCAAAAATATTATTATTGGATGAACCATTGTCAAACTTGGATGCTAAGTTGCGTATTTCAACAAGAAAGTGAATACGTTCAATTCAACAAGAATTAGGAATTACAACCGTTTTTGTTACACACGATCAAGAAGAAGCTATGTCAATTAGCGATAAAATCGTATGTATGTCAACTGCTAAAATTCAACAAATTGGTTCTCCTATGGAACTATATTTGAAACCTAAAAATGAATTTGTTGCCAAATTCTTAGGTATGCCAGAAATGACAATATTTGAAGCGCAAATTAAAGACGGATATATAATTTACAACAATAAAAAACTACTTAAAGCTCCTGAAGGATATCAAAAAGCTTCTATTGATGTTGGTATAAGAGGCGAATCTCTAATAGAAGAAGAAGAAGGAATATTTGAAGGAAGCATAAAATTAGTTGAACATTTAGGTAAGGAAATTCAAGCTCAAATATTTATCAAAGAATTAAATAAAATTGCAAACGTTTTCTTAGCTAAAAAACACAATTATGAATTAGGTGAAGTTGTTAAGTTAAATGCAAAAAGTGAAGGCTTATTCCATTTATTTGATGTAGACAGCAAAGAACGTGTATAATGAAAACCTATTTTTGAAAAAAATATAGAGTTAAAAACACTGGACTTGCCTTAAGCACATTAAATAACAGGGTTAGTTTTTGAAAACCCTTCTTATTAATGTTGCCATCATTAATTACATTAACTTTATTTACTATAATTCCATTTATTTTGGTTTTAATCTTTTCATCAACATACCAAGTTGGTGCGACGGTTTATGACAAGGGATTTGGATTAAAAAACTATATTAAATTGTCAAAAGATCCAATATTCCACATTGCTATTAGAAATACATTGGTTTATGCTATATTAGCTTTACCAATTAGCTTGGCGATTTCCATTTTAATTTCATCTGCAATAAGCTTTGTAATCAAACAAAGTTTCCGTAGATTTTGACAAACAGTATTCTTTCTACCATATGTAACATCAGGAATTGCTGTTTCAATCGCCTTTGCATTCTTATTTAGAACATCAGGCGGATTTATTAACATGCTAGCGGGTAAAAACATCCAATGGCTAGATAACCCAAATGATGGTAATTGAAATGCATTTGTTGTTATTTTAATTAGAGGTGTATGAGGAAATTTAGCCTTTCAAATTCTAATTTTAACAACCGCTATGCTATCAGTTAACCCTGATTTATATAAATCAGCTTCTATTGATGGAAGTTCAAATTTAAAACAATTTTTTGCAATTACTTTGCCATCAATTAGAAAAACAATTACTTTCCTATTTACAGTAGGAATTATTGGATCAATTAAAACATTCCCATTGGCTTTATTTGATAACAAACCTGCTGAAGCAGCTGCAAACTCTGGTATGAGTATTATGTTATACATATTCTGATATGTTCAACGTGGAGTAAATGGCGTTGCTGGAGCAGCATCATTGATACTATTTCTATTAGGCTTATTAGTATCATTTGGTTTAAGAAAATTAGTAAGCTTAGTGTTCTTTGCTAACAAGAAGATTGGAGAAATAAATGTCATTAGGAAAATTGAAAATAAAACATTGAAGAGAAAAGTCGTATTTAAGATCTAAGCAAGAGTTAATGTCGAAACCTTACTCTTCAACTAAGGTTAGTTCATTAATAGCATCTTGAGCATTTAAAATAATAATTTTAGTATTCTTCGCGTTAGTAATAATATTCCCATTCTACTTTATGATTGAAATGTCATTTGTAGAAAAGCAATTAAGTACAGATTCAAGAACCACAATACTATTTCCAAGAGCAATAGTAGAAGGTGGAAAGAGCTTCTTCCCTGAAAACTATAGAGATGCTTTTGAATCAGGATTCCTTGAAGCACTAGTATTTACTGCTGCTATCACAACTTTCTCGGTATTAATTAGATTAGGATTTTCAATTACATTGGGATATGCACTATCATTAAGAAACTGAAGGGGAAAGAATTCATTCTTTATCTTCTTTATATCACTAATGATATTGCCGGAAGTCGCCTTACTAAGTGGTCAATACCGGTTAGTTGTAGCATTGGGTTGAACTATTGGATGAGGTCAATTCTTTGCATTAGTTATGCCATTTGCCGCTTCAATATTCTTTGGATATATGTATAAAAATGCATTTGAAGCAATCCCAAACAGTGTTAAAGAATCATCAATGCTAGATGGCGCAAATGGAATCAAATACTTCTTTAAAATAGCAATGCCAATGGTTTCAGCAACAACCTGAACAGTTTGCATTCTTACAGCATTTGCATCATGGAACTCATATACATGACCTGCATTAATATTTAAAACCTTAGGTTCACCTTGAAAACCTTTAAACCTATGAGTATTTACTACCGGTAAATCACAAGAATCAGAAATTCAAATTGTTTATACATCAATAAGAATGGCTGCAACCGTAATTGCAATTCTCCCAATGTTTATAATTTACTTCTTATTAAAGAAGAGAATTATGAATGCAATTTCAAGACAAGGAAATGCAACAAAAGGATAGAATTATGGAAAAAGACATTAATGTTCAATTAGTAAAATTATCGAAATATCAAAAGAAAAGTAAAATTAAAAAAGTATTTATTTATATTGGTATAGGATTTGTATTAATTTCTTTGATTATTATAATGATATTGGGTGTATATGGCATAATTGGTGGTCTAGACGGATTAATAAAAAAAGGTTTATAAAAAACTGTAATAAAAAATATCGCATTTGCGGTATTTTTTATTGATATTTTTTAGTTTGCAAATATAATATTTAAACAAAGTCAATAAAAAATTTGGAGAGATATATGGCCTATGGAACTAAAAATTTATTAATATTTAAGAATAATTTAATTCAAAATAAACAAAATTTAAAATCTTTTGCACTTAAATTAAGCTTTTATGTTTTAAAACAAATGCCACAAATTAAAAGTGTTATATTGGCATATGACAATGAATATTGAAAAAAAATGAGTCAATACGTAACTTATTATTTAAAATCTAGGGGTATAAATGTTTATTTTTTACATAAGAATCATTTAGGAGTTCCTTTTTCATTCTTGGAATTTGCTTTTAAAAAATTAAATGCCTTGGATTTGGCAATATATTTTTCTTTTTTTGACAGTAAATCATGTAAAATTCAAACTATTTTTAAGAATAATCAAGAGATAAATTTTTTTGATTTTAGTAATTTTAACCTCAACGAAAATGAATACCAATTTTTAAATGATGAGCCAATATATTTAGATACTAAGATGCTGCTTAATGATTATTTAAACCAAATTACTTCAAATAGAGTAACTAAATATCATAATTCAATCCTTAATTTAATAATTAACAAATATCAATATAATTTTGAATTAATTAAGAAGATTATGGGTTTTGATGATTTGGGATATAAATTTATTAATAAACAAAGATTCACTAATGATACAATTTTTAGAGTTTCTAAAACTTTTTTAACTAGGGGCATGCAATTTAATTATTTTGCTAGATTTTTTATAAATCAAGAAGTTAAATATTATTTAGCATATAAAAAATTATTTAATTATGATTTTAAGCAAATTTTAAATGAACATTTAGTTGCATTATATATAAAATTTCAAAACACATTCTTTAGCTCAATGCTAATAAATGATAATTTTTTTAAAATAAATAAAGTTTATATAAATCAACTTTATCCCACTCCTATTTTAATAAAAGTTTGTGAAATGTTTGACATAGAATATAGTTTTGTTTTGCAACAACAAGACATTATTTTAAAAGACAATGAAAACGTTTTGTATGTTGATAATAATGATATTTCATTTTTAAGTGCTGAGTATAATTACAGAGATTCATTTAAGACATTTTTTTATATTAATGAAATGTTAAACTATTTTGAAACTCAGCAAATTGATATTTTTGACATTGAAAAAGAATTGAAATTAAAATTCTTAACCTATAAAGAATTTGAAATTGAATGTAGTATTGCAAATTTAGTTCATTTTAAAGATAAATTAAGTATTCAAAAATCAATATCTCGTATTGAAATTGATCAATTAACAGTTTTTGAAAACAAACAAGACAAATATATAAAACATATTGCTAAGATAACGTTAAAAACCAATCCTCAATGTTTTATATCTATTAGTTATAATGAAATCTCCTGCAAGTTGAAATTTTCTGTTTTTTATTCGCAAACTACAAAAATTTTCAATAAAATCAAAGAATATTTTAATAAATTTCTTTTATTAAACAAAAAAATTCGCAACTAAGCGAATTTTTTAATATTATTCTTGGAACAAAAGCTTAATTTTATTAATAAATTCATCGTAGTCAAAAATTTTTAAAACATTGGCATCACTCAATATTGATCTAGCTGCTTGCTTTATTGCATCTCTTAATTGACCACTTCTTCTAGTTGCAACATCATCGGCAGGTTTGTAAATTGATCCTTCATTGATTAAATGTTTAAAGTTTGTGAATGCTATTTCTTCGGCAATATTTTTTGTTACGCTCTTTGCATCTTGGCTTAGATATTTTTTTGTAGGAGAAATATAACCACTAAAGATATTAAAAGCATCTATTGCTTTTGTACCATCGGGGAATGCTTGAACATTTTTACCACTACTATCTTTATAAAATTTTAAATTATTTAAAGGTTGTGTATACATTCAATTAATAAATAGTTTAGTTGCTTTATCTTCTCTTTTATTTGCATGAACCGGGATTACAGAGGGACCTTGGAGAATAATTCCTCTATATTTTTCATCTTTTGAATATGAATAAGGGGGTGATATTATATCTACATCTTCTTTGTTAATAAAATTATTAGCATTCAATGAATTAACTTCCACATCTTTTTTGTCAATAAAGAAATATTCGTTATTGTCATTGTTAAAGAATTTTCCTAAATAAATTCCTTGAAATTTAGTTATTTTATTTCCAATTTTTAATTCGATTTTATCTTCAACATTGTTGTATGAATATTCAGGCCCCGAAACAATAAATCCTGCCTTGCCTTGTAATTTTTTAGCTTCGTTTGTTGATAAAGAAGATATTTTTTTAATATATCTAGGATTTTGTCCGGCATCTTTTATATCTTTTTCACTAGCATCCGCTGTTCAAATTGTATTCGAATATTTATCATTCAATTGTTTAAATTTCAATAATCATTTTTCATTATTTTCGGGAGTATCGTTTAATATTTCAATTGTTGTGTTTGGATTTAACACTCTGTTTGCCTGCTTGATTGTATAATTGTTATTTTTTCCATTTTCACTAACAAAAGTGTAATGATATGCAGCTGTTGAAGTAATAACCATTGCTAATTTATAGGTGGTAAAAATGCCCGTTCCTCAAACACCTTGTTTAGCTACTCAAACGGCACCTTCTTGAATACCTGTTAAAATAATAGAAGAAACTTTTTTAAATAATTTCGCTTGTGGGCTATCAGGATTTTCAATAAAATTGTCATAATTGTAGCCACCAGTTGTTAGGCAATTATCATCTGGGTTTATATAGTTTTTATTGATATCACTACCAATGATTGATGTTGCCATAACATTGATAGCCGAAGGTATTTCATCTATTCCCAAAACGTTTAATGTAGGGTCCGCGTTGTATAATCTTTTTGCTTGAATTGCAAAATTAATTAAATCGCTATAGTTGTCAAATAATTTATCTGACATGATTGGCAATTCTTTTAATATTTCAGGTGTTAATTTTTTTAAATTTTCTGGTGTTGTTTTTGAATATGCTCAAATACCATCAACGCCTTTTGCTTCTTGTTTATTATTATTGTAATAGTCTAAATAAGATTGCAATTTGCTGCTATTTTGACTATCTAATTTAACTCCTAATTCCATTAATTCTTTAACGAATTTTCCAACTAATGCTTTAGCTACTGTAGTCATTTCTCCTGAACGAGATAGAGGTATTGCTCATTTTTTGTTATCTTTATTATTTGCAATTAAAGAGTTTGCAACATTAAATACTGGTTCAATGTTGTATTTTTTAAAATCTTTGTCATCAATTGCTAAATTCATTTTGTGATTTGCATATAAAGCAGCTGCTGATGCGTAATTTAATAAAATGTTGTGAAAATCTTTTCTGTTTTTTACTTTTAAATCAAGTGCAATTGAAGAAGTATTATATCCTGAAGCTAATCAGGAAATTTTTAAAGGCAAGTATCCTTCAGATTGCTTGTTATTGTCTTTCATTCATTTGTTGTATGCATCAACAATCCCTTGAATAGCAAGCCCTTGCCAATCGTTTTTTAAAAATCCCATTGCTAATACTAATTGCCCATCATCTTCTTGATCAAATCCGTATTTCGAATTTTCTGTTGTTGAACATGAGGATAAAATCAATGTAGTCGTAGGAAGTAAACCTATAGTCATTAAAGTGAATAATTTGCCAAGTCTTTTCATATAATCTGTTTCCTTTAAAAATGTATAAGAAATGAACTACAAATTCATATAACCGTGTTGTTCTCTACTGGCTTTAAAACCATGTAGTAATATAAAATATATTTTAAATATAAACCCAAATTTCTTATTTAACTAATGAAATAAATTATATATTTTTATTGCATTAATTTTTAGAAAATAGAATTCTTTATGCACTTTTAGAAACAAAAGAATTACTTTATTAAATTAAAAATTCGCAACTAAGCGAATTTTTTTAATATTATTCTTGGAACAATAGCTTAATTTTATTAATAAAATCTTTGTAGTCAAAAACTTTTAAAACATTGGCATCACTCAATATTGATCTAGCTGCTTGCTTTATTGCATCCCTTAATTGATCGCCTTTTCTAGTTGCAATATCATCAGCGGGTTTGTATATTGATCCTTCATTAATTAAATGTTTAAAGTTTGTGAATGCTATTTCATCAGCAATATTTTTTGTTGCACTCTTTGCATCTTGACTTAGATATTTATTTGTAGGCGAAATATATCCACCAAAGGCATTGAAAGCATCTATTGCTTTTGTACCATCAGGGAATTTTTGAATGTTTGTTTTTTTGCCATTGCTATCAACTTTATAAAGCTCTAAATTATTCAATGGTTGTGTATACATTCAATTGATAAATAATCTAGTTGCTTTATCTTCTCTTTTGTTTGCATGAACTGGAATTACAGAAGGACCTTGAAGAATGATTCCCTTATATTTGTCATTATTTGAATATGAATAAGGTGCTGCTATTCAATCTGCATCTTCTTGGTTAATAAAATTATTGGCCTTTAATACTTTAACATCCACGTCTTCTTGATTAATAAAGAAATATTCATTCTCATTATTATTAAAGAATTTGCCTAAATAAATTCCTTGTAATTTAGTTATTTTATTTCCTATTTTTAATTCAATTTTATTTTCGACTTTATTGTATGAATATTCAGAACCGGAAACTATATAGCCTGCCTTGCCTTGTAATTTTTTAGCTTCATTAATTGATAGAGGAGATACTCTTTTAATGTATGTAGGATTTTGTTTGGCATCTTTTATATCTTTTTCACTAGCATCTGACGCTCAAATTGTGTTTGGATATTTTACACCCAATTGTTTAAATTTCAATAATCATTTTTCATTATTTTTAGGAGTATTATTTAATATTTCAATTGTTGTTGTTGGATCTAATACCCTGTTGGCTTGTTTAATTATATAAGTACTATTTTTTTCATTATTGCCAACGAATGTGTGACGATATCCAGCAGTAGAACCAATATTTATAGCCATTTTATAACCAGTAAAAATACTTGATCCATAAACGCCTGGTTGAGCTACTCAAACAGCATTTTCTTGAATACCTCTTAAAATAATAGAAGAAACTTTTTTAAATAATTTCGCTTGTGGGCTATTAGGATTTTCAATAAAATTGTCATAATTGTAGCCACCAGTTGCTGGGCAATTATCATCTGGGTTTATATAGTTTTTATTGATATCACTACCAATGATTGATGTTGCCATAACATTGATAGCTGAAGGTATTTCATCTATTCCTAAAACATTTAATGTAGGGTCAACATTATATAATCTTTTTGCTTGAATTGCAAAGTTAATTAAATCGCTATAGTTGTCAAATAATTTATCTGACATGATTGGCAACTCTTTTAATATTATAGGTGTTAATTTTTTTAAATCTTCTGGTGTTGTTTTTGAATATGCTCAAATATTATCAACGCCTTCTGCTTCTTGTTTATTATTATTGTAATAGTCTAAATAAGATTGCAATTTGCTGCTATTTTGACTATCTAATTTAACTCCTAAAGCCATTAATTCTTTAACGAATTTTCCAACTAATGCTTTAGCTATTGTTGTAATTTCTCCTGAACGAGATAGAGGTATTGCTCATTTTTTGTTATCTTTATTATTTGCAATTAAAGAGTTTGCATTATTAAATACAGGTTCAATGTTATATTTTTTAAAATCTTCATCATTAACTGCTAAATTCATTTTATGATTTGCATATAATGCAGCTGCTGATGCGTAATTTAATAAAATGTTATGAAAATCTTTTCTGTTTTTTGTTTCTAGGTCTAATGCAACAGAAGAAGTGTTGTAACCTGCTGCTAGTCAAGAAATTTTTAAAGGTAAATATCCTGCTTGTTTTCTTTCTTCTTTGAATTTTGGATCATTTATTCAATTATTATATGTATTAACAATTCCTTGAATGGCAAATCCTTGCCAGTTACTTTTTGAAAATCCTGTTGCTAATACTAATTGTCCGTCATCATCTTGGTCAAATCCATATTTTGAATTTTCTGTTGTTGAGCACGAGGCTGAAATTAATGCAGTTGCGGGAAATAATCCTGCCGCTACTGCTGTAAATAATTTGGTAATTTTTTTCATATAATTTATTTTTCTCCTAAAAAAAGTGTATAAAAAAACGAACTACAAATACAAACATGTTGTACAGTTATGTTCTTACTAGTTTAAAAAACGCTAGTAATATAAATATATATATTTTTTATTTTTTGTTTCAAAAATTTTTATTTTTTTTGAGCCAAAAATATAAATTTAAATTTTAAATTTTTGCTTGTTAAAATAAATTAATATATTGTTATTACATTATTTTTAAAAAATAGATTTTTTTATAAAAATTAGTGTAAAATAAGCATGCAATGACTCAGCATAAAAATATTTTTTAAATAATTTTTGCTACATTGGATTGAGAGTAAGAAAGCTGTAATCCGAAATCATTTAAGCAAAAATCTTAGGAAAGAATTTTTAAAGTCCTTGCATATGAAAGGAACTAAAAAATGTCAAAATTTGTTGGCTCAATGTTTAAAAAGAGCCGTAGATATGGAATTTCTTTATTAGAAAATAATAAAGAATTCACAAAAGGCAAAAAACGTACAACTGCCCCTGGACAACACGGAGCAAAAAGAAGCAAACCATCAGACTATCAACTACACATGTATGAAAAACAAAAAGTTAGATATATGTATGGTTTAAATGAACGTCAATTTAAAAATTTATTTATTAAAGTTTCTAAAAAAGCCGGAGTTACTGGTATTAACTTACTACAAACTGTTGAATCAAGATTAGACAATCTAGTATTTAGAGCAGGATTTGCAAGAACAAGAGCTCAAGCAAGACAATTAGTAAATCATGGACACTTCACAGTTGATGGATGCAAAGCAAACATTCCTTCAATGATTGTTAAAGTAGGTTCAGTTATTGAACTAAAAGAAGCATTACAAAATAATGTTGCTATTAAAGATGCTATTGAAGTAATGACAGTTGCTCCATGATTAACAAAAGAAAACTTTAAAGTAACATTTACAAGATTGCCAGAAAGAAACGAATTTGCTAAAGATATTAACGAATCATTGATCGTTGAATACTACAACCGTTAATAAACAAAAAAACGAAGTTTGAACAACCTCGTTTTTTATTATTTATTTTTTAAATAATTAACTTTTTTAAAAAATATAAATTGTTTTTCTTTTGTTTTTGTAATTAAAACAATAAATAAGATAAATGCTGGTATATAAACTAAATTAATTAGATAAATATTATTGCATAAAGGATTAAGCGTCATTGTTATATTTGAAAGTGAAAACAGCGTATTTAAAATTTCAGGGAAAACTATAGAACTTAAAACAATAATCAAATAGTTTGTGAGTCTATCATGACTAATTTGATAAAAAAGAATACCCAAAATAAGTGATATTAGATATATTAATATAATTACAAAAGTAAACAATATTGCTTTTTTGAAGTTATTGGTGATTTTGATTAATAAAAAACTTAATAAAAATCAATAGGTTAATCTAAAAATATAAACATAAAAATATTCAATAATTTTTGTATTTTCTAAATATACAGGAGTTTTTGTATTTCAATGTTTGTAGGCGATTTCATAAAAATTTTGTTTAATAAACAAAATCATTACGTTAATTAAAAATATTACAATCAATGATATTGCAGTCGTCAATATTATTATTAAATAGTTTCTTCTTTTTTCTTTGTGATCTAGTTCTATACTTCGTTTTGATAGATATCGTTTAACAAAATAGAAGTTTAAAAATAGTCCTATTGATAATAATGTTAAAAGAAGCTGGGTAGTATAAGAAGGAGCGATTAAAAATATTTGATTTATTAAAGGAAATAGATTAAATTGATCGATTTGACCATCTATTTTTAATATTATTGCTGATATTAAAAGATAAATTACAAATCCTGCTAGCAATATTCAATATGGCAGAAATAGTAGTAATTGTTTTTTATTTTTTTTCATTAACAAATTTCTCCATTTCACTATGCATGGTAAATAAATGAATATATAGTTTTAGCTCCATATGCGGTAATTGATTTTTATTTTAGCTGTTGTCAATATCTAATTATTGTTTAGTTTCTTAGATATAATATCATTTGCTTTTTCAATTCATAAACTCATTTGGTTATGAATGTAATTGTAAAATCAACTAGATTTGTTTAAATATTCCTCATTAAATTTTTTTAATTCAAAACTAAAAGTATTTGTTGCTTCTAATAGTAATGCTTCGTATTTTGTATTATCTAATGTTTTAAATAGTTGTTCAAGTTCCTTTTTTGCATCTTCTGGATTGCAATATATATAAAAGGGTTTAATAATATTATTTTGGATTTTTGTCATAATAATAAAGCAATAATTAAGGTCTTCATCATTGACAAAATTTAATAGTTTTTTTATTAGTTTTTGAATAATATAACTTGCTTGGTAATTATCTTCATCATTTGAATTGCTGAATTTAAATTGGTACTCTGTTTCTTCGTCAAATAATTGATTATCAGCTACAAATGCTTGGTTTATATCGCAATATAAACCTTGAAAATTATCAATGTCATATTGACTTAAATTATTTGATATTGAAGTTAGAAGTACTTCATTATCGTGTTTAATTTTTGAAGCCTTTGTATAAATTGTAAATTCGATTGTTCTAACAAGCGGAATTAATTTATATTCATCAACATCATTCAAAGGCAATAGTAATCTAGAACAAATCATATATTATCTCCAATCTAATAAAAAAACCACAAGACATAATACTTCTCGATATGGCTGCTATATTTCTATCCTGACCAAGTTACAAGGTTATCATTCTCATGGCATAATAATTATACTAAAAAACAACTATTAATACAAAAACTATTCAATATTTTGATTAATTTAACAATTTGATTCTTATTTTTTGTTGCCCTTGTAGTTGCTATTGTTTGGATTCAATTGATTCGAATGATTGTTTAAATTCGATCTGTATGCTGAATTATTAGAATTATTTTGATTTGAATAATTATTCATTTGTTGTCTTGTATGTGTGTTTCCACTTACTTTATTATTTTTGTTATTCATAATTTACTCCTAATTTTTATATTCATTCGGCAATCCATAATGATCACAAAGCACAATGTATGCCGCCATTTTTTTAGCAAGTTTTTTTGATGTGCCATATCCTTCTGTATATTTAGTCCATAAGCCCATTCATTGAAGATTTCTAAAATTAACGTTACATCTGCAATGTCATCATTGTTTGCCGTTATCATCAATTATAGGTTTTTCTGATATTTGATATATTGGCATTGAACATTCACCACGCTCTGCTAATTCTTTTAATGTATTGATAGCATTTTCTAATTTAAATTTATCCGGTCTTTTTTCTCTGCAATCAACATCTCTAAAAAAATGTTCAATATCTAACATCTTTATAATGATGTTTTTTAAACGCTTAATATCTCAACCACTGTCAATTGCTATTGCACCAATTATAGCTTCAAATAAATCAGCTTTCACACTATCTTGATTAGCAACATTATTTCTTTTATCATTATCTCCTAAAACTAAATACTTAGCAAAACCTAATTTATTAATTTTTTTAGCTAGTGTTTTATTGCTTACGATTTGATTTTTTAAATCAGTAAAATCGGATTCATTTTTATTGGCTATAAGGCAATATTCTTCAAGATCTTCATATTTGTTGTAATATTGCGAAGCTGATTTCAAAAAGCCAAATTCTTCTGCCATTAGTTGAACTACGCAAAAGCTCAAAACTGAATCTCCTATAAATTCAAGAACTTCATTATTTTCAATTCCATATTGATTTGAATAAGAACTTCTGGTAAACGCTTGATAAAGTAAAAATTTATTTTTAAAGGAATAACCTATTGCATATTCAATTTGATAAATATATTCTTGCCATTCTTCTATTCCCATATTTGAATTTTTCCTTTCTAAAATTTATTATTGTTCAACAGTTAAAATATGCTTTTGTTGTATATTTTAAATTCTATTTAATTAAGGTAAAATAATTTTTGTAATATAGTTCATCTAAACTTCTTTATTTAATTGTAAAAAAATGTAAATAATACATATTATTCAACTACTTTAACATAACTAAATTATTGAACAATTTAATTACACTTATATTAAGCTATGATTTTATTGCATTCAAGAATAAATTTAAATGTATGTATATTGATTTTGTTATTTTGGTTTTTAAAATAGAATTACAATATGAGTTTTATTTGATTTTAGCGTTTTATAATAATTTAAATAATATAATATTTATGTATTTAGGAGAAATTATGTCAAACGAAGAAAAAATTTATGTTTTTAGTTATGGAACTATTCAAGATCCACAATTTTATAAGGAATTATTACCAAACTCAAAGCCAATGCCAGCAATATTAAATGGTTATGCAAAATGTGTTGATGAAACAATGTATTTTTTATTAAAAAAAGATTTGTCAAGTCAAGTTAAGGGAAGCGTATTTGAAATTAGTAAAGAAGAATTGTTTTTAATTGATAGATGGGAATTATTTCCTCAATATCAAAGATTTCAAGTTAATGTTTTATTAACCGAAACAAATGAAATTTTAGAAAATGTTTATGTATATACAAAATTAGAAGTTGGAAAATATTATTTAGCAACAGATGATATGGGATTTTCTAGAAATCCTAACGCTAATGAAAATAATTTAAATGCTTTTATTGAAATGGAAAAGGCAATAAAAGATTTTCCATTAACTGACTATATATTTTTATATGATATAAATGAACAAGAATTTGAAGAAATTAATAAATTAACTCACCCTTATGCTGCCTTAATTATTGATGATAAGGAAAATAGAAATTATGTTGCAATTCATGGATCAATTTTTGCAATTAAAGAAGATGGCAAAATGTATGGAGCCTTAACTTCGTTTAGTCAAAAGTCAAATTTAAATAGTATTTTTTATTATCAAGCATTTAATGAAAAGTTATTAAATAGCAAACCAGAAATAACGTTAAAATCGTTATACGATAATACAAATATAGATTTTTTAATTAATAAAAAACCTGTTTATTATTTAAGCTCAAGAGAAGATAAAACAATCAATGAAACTCAAGTTGGTTGATACGAAAATAAAGCCTTTGAATTAGTTGAAAAAGATTTTGACATTGATCCATTTATTCGCTTTAATAAAATGTTAAAAGCATTTTTTGATACAAAACAAAAAAATGATAAATAAAGAAATAATTATAAAAAATGTGCCACATAAACCTGGAGTTTATTTATGAAAAAATAAAAATGGCAATATTATTTATGTTGGCAAAGCAAAAGACTTGAAAAATAGAATGTTGCAATACTTTGATATAAAAATGCAAAATTCTTTTAAAACAGAAAAAATGAGGCAAGAGATTGCTGATTTTTCTACAATCATTTTAAGCTCGGAACGTGAAGCATTTATTGAAGAAAGAAAATTGATTGATTTTTATAAACCATTTTACAATGTTCTTTTTCCAACTAGAGATAGTTTCCCTTATATAAGAGTTAAATTACTAAGTACTAATAAATTAATAATTGACATAAAAAATCATTATAAAAAAGAACCAAATTCTATATATTATGGTCCTATTCCCGACAACAAAAATTTCAAACCATTAATTAGATATTTGAATCATTTACTACTTTCTAAGGATGGAATCATAATTGAAAAACAAAGTCCTGAATTTGCAAAGCAGAGATTTGAACAAGCCAAATCAATCATGAAATTTGGTCCTGATTTTAAAAGAAATTTAAAACAAAAAATCAATGAGGCAATTGAAATGAACCTGTTTGAGCAGGCGAGATTTTATGACCAAATTTTAGATTTATTAAATTACAATAAACAAAGTCAAAATATTTTTATAAAAACTATCAAAGATATTGATGTCTTTGGTTTTTATCAATATCAAAACTATATGTTGGTTAATGTTATGATTTATAAAAACGGATCATTAGTAAATTCTTATGACTATATTTTTGAAATAATATTGAATGAACATGAATTTGTTAATCAATTCATTGATGAATTTTATGTTAATAATCAATTACCTGATCAAATAATAATACCTTTAAAATATTATGAATTAGGTGGATTTGATTATAACGACAAAGTAAGCGCGCCATCGAATAAATTAAATAAAAACTTAATTGATATTGCTATTGAAAATGCATCAAATAATATTGAACAAAAAATAAAAAATTATTTGAGTAAGCAAACAAAAGACAATGAAATTTTCAATAAATTATGTTCATATTTGAATGTTTACGCAGAAAGAATTGTTTTATTTGACAATTCATTTTTATCAAATACTAACGAAGTAATAGGAGCAGCTTGCTATTTTAAAAATGGCCAAGCTTTTAAAAAATTCTACCGTTTCTATAATTTAAAATTGAATTTATTAAGAAATGCAGATGTTGAATATATGCAACAAACTTCCTTTAATTATTTAAAAGATTTGGGAGATCAAGTTGATGTTGTTATTGTTGATGGTTCAATTGGTCAAATAAATGAAGTTAAAAAATCAATGGCATTTTTAAATTTAAATATTCCTCTATTCGGGCTGGTTAAAAATAGTAAACATGAAACTCAAAAATTAATTGATTCAAACAACAACGAAATTAAAATAGATGACCAAGATGTATTTAATTTGCTTTCAAGAATGCAAACAGAGGTTGATAGGTTTGCAAAATATAGATATAACCAAAAACAAAGAAAATCAATGTTATCTAATCCTCTATTAAAAATAAAAGGTATTGGATTTAAGACAATAGAAAAATTAATCAATTATTTTGGCAGTTATAAAAAAATTCAACAAGCTTCTTTTGATGAAATATCAAAAGTAGCAAACAAAAAAGTTGCTAAGCTAATTTGTGAAGACAAAGGGGAATCGTTTTATTAAATTATTTTTCAAAAATATTAAAAAAATTTTTAAATGGTGTAAAATAATAAAGCAACAAAATAATGGTCGTGTAGCTCAGGGGATAGAGTACGTGCCTTCTAAGCATGTGGTCGCAGGTTCGAATCCTGCCACGATCGCCATTTTTTTTATTCATTTTTTTAAAAAAATAAAAAATATGTATATAATTATTAACGCATACAATTAAATATGCGAGTGTAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGAATACGTGGGTCCGATTCCCATCACTTGCACCATTGCGGATGTAGTTCAATGGTAGAACATCAGGTTGCCAATCTGAATACGTGGGTCCGATTCCCATCATCCGCACCATTTGAGTAAAGTTCACCAAAATTGACATAAGCTAAGGTTTGTGTCAATTTTTATTTATAAAATTGTATAAAAATTGTTACTGCTATATTTTATAAAATACTAAGTTTAACGGTTATTATGGTTAATAAACATCGGCTCTATTTGTTATTATTTTCTTTTTCTTATATTGAAATAAGTATTTAATTATGAGTTTAAATTTTGCTCTATTTTAAGACTATACATCTTTTTTGTTTTTAAAACATATTGATGTTTAATTTATTATAATTAACTTAATATTTAGCAAATACATTAATTGACTAAATATTAACCTTAAAAATATAAAGGAAAATTATGGCAAAAGTATTAATAATATATGGTTCAGTTATACCAAGAGAAAATTCAATTTCAACAAAAATGTTAGATCTTTTTGTTGATGAATATAAAAGAAATAATATTAATGATGAAATAAAAATTCTTGATTTAAATAATGAAGATATGTATTCATTAACTGACAAGAATTTTAAATTGTTTTATAGTGAAGAAAACTCTGACAAATATATAAATTTATTGAAGTCTGTTGATAAAGTTGTTATGAATGCGCCGATGATAAACTTCAATATTCCAACTATAATCAAGGCATTTTTTGACCGTATTGCAGTTGCAAATAAAACATTTTCATATAAATACGCAAGCAAAGGTTCTTCAATTGGTTTATTGAATAATTTAAAAGTTCAAATAATCGCAACGCAAGGAGCTCCATTAGGTTGATATCCATGAGCAAGTCATATTAATTATCTTGAAGGAATGTGAAAGTTTTTAGGTGCAAATCTTTCAGAAAGCATTTTATTAGCTGGCACAAAAGTTGAACCTTTAAATAAAATGCAACCTCAAGAAATGATTGAAACTATTAGCAAAGAAATTATTAAAAAAGCAAGCATATTTTAATTATTTTTTGTTATAATGTAAATGCAATTTTTATTGCCACATTAAAGTGGAAGAATTTGAAACAAATTCGTTTGAACCACTATAATCGAAAGGATTTTATTTATGGCTAAAAAAGTAGTCAAGAAAGCAAAATTACAATTTGCTGCCGGCCAAGCAAAACCAGGCCCAGCATTGGCTGGTGTTGGTATTAACATGCCAGAATTTACAAAGGCATTTAATGACAAAACAAGAGAAAGAGGAAATGAACCAGTTCCTGTTTTAATTACTGTTTATGAAGATAAATCATTTGAATTTAAATTATTTACTTCTCCAACATCATACAAGTTAATCGAAGCTGCCAAAATTAAAAAAGGTAGCGGAGTTGCAAACAAAGAAAAAGTTGGTTCTATAACAATTGATCAATTAAGAGCAATTGCAGAATATAAATTACCTGATTTAAATACAACTAAAATTGATTCAGCAATGAAACAAGTAGCTGGTACTGCAAAGAATATGGGAATTACAGTTGAAGGTTATGACGAATGACTAAAAGGAAGTGCTAACTAATGGCTAAAAGAATTACAAAAAACATTAAAAATGTTAAAAGCTTAGTTAATAAAAAAGACGTATTCCCTCTTGCAGATGCTATTGAATTAGCAAAAAAAGCTTCATTTGCAAAATTTGATGAATCTGTTGATATTGCAATTAGATTAAATCTTGATACAAGAAAATCAGATCAACAATTACGTGGATCAGTTGTACTTCCACACGGTACAGGAAAATCAGTTAAAGTTTTAGTAGCAACTGACGAAGTTTCAGCCCAAAAAGCTTCATTAGAAGCTGGAGCAGATTTTGTTTACAGTTCAGCTGAATTACCAGAAGTATTAAATCAAGATAAATACGATTTTGATGTAATAGTTGCTGATCCTAAAATGATGCTAGTTTTAGGAAAATTTGGTAAAAAATTAGGACCTAAAGGTTTAATGCCAAACCCAAAAACAGGAACAGTTACAATCAATCCTGCTAAAGCTGTTGAAGAACTTAAAAAAGGTAAAGCAAATTATCGTGCTGATAAGGGCGGAATTGTTCATGCCTCATTTGGTAAGAAATCAATGGAAACAAATGTTTTAGCAGAAAATGCCGATACTATTATTCAAACAATTAAGAGATTAAAACCTCAAACAGTTAAGGGTACATATGTTTTAAATATTACCGTTTCAACTTCAATGGGTCAATCTGTTAAAGTAAGAATTGATTAATATGTCGAAAAAAAGCAGTCTTAACGCTGCTTTTTTATTTGTTTTTAATAAAAAACAAAATCGTTAAAATAAAAAATACCTCAAAATTAGAGGTATTTTTTAAATTGTGTTAATTTTTTAGATTATAAAATGAGTTTTTGCCTTCGAATTTAGCTTCATTGCCTAATTCTGCTTCTATTTTTAACAAGCGGTTGTATTTAGCGATTCTATCTGTTCTTGAAAGAGAGCCGGTTTTGATTTCTCCGGTATTTAATGCTACTGCCAAATCTGCAATAAATGTATCTTCAGTTTCACCTGAACGGTGAGAAATTATTGCTACAAAGCCAGCTTTTTGTGACATTGTTATTGCTTTAAATGTTTCAGTTAATGAACCTATTTGGTTTATCTTTATTAAAGATGCATTGATTGCATGTTTGTCAATAGCCATTTGAATGTATTTTGGATTTGTTACAATTAAGTCATCTCCAACTAATTGAACTTTGTTGCCAAATCTCTTATTCATTTTTATAAAGCCATCTCAATCGTTTTCATCATGTGAATCTTCAATAGAAATTATTGGGTATTTCTCAATTAAATGGCCATAATATTCAACAAATTCATCGCTAGTAAATGAATATTTAACATTTTGATATTTTTCAAATCCTGGACGTTTTTCTTCAATTGCTTTTTTGAATTTTTTAAACACATATAATTTTGTATCTGAATGATAAAGTTCTGAACTTGCAGCATCCAAACAAATTGCTACTGCTTTTTCGCCTGAAGTAGCTGGATTATATCCTGCAACTTTAATCGCATTTACTAAAAAGTCCAATGCTTCTTCATGAGTGTGTAGGTTTGGTGCAAATCCGCCTTCATCTCCGACTTGTGTTCCATGACCTGCTTGTTTCAATAATTTTGCTAATGTATGAAATACGAAGTTTGCCATTTGCAATGATTCTTTAAATGTTTTTGCCCCAATAGGCATTATCATAAATTCTTGGAAGTCAATAGTATTTGAAGCATGTTCTCCACCATTAATAACGTTTAGCATTGGCACTGGCATTTTGTATGCTACTTGGTTGTTTAATTTTGCCAAATATTTATATAATGGAAGATTTGCTTCAATGGCACCAGCTTTTGCAATAGCTAATGAAACAGCTAATATTGCATTTGCCCCTAATTTTGATTTTGTTTCAGTTCCATCAAGATTAATCATTAAATTATCTAATTCTTCTTGCTTAGAAACTTCCTTGCCAATAATAGCTTTAGCAATAATATTATTAACATTATCGCATGCTAATTGCACACCTTTACCGCCAAATCAATTATTTTCGTATTTTGTTCCTTTGTCGCGTAATTCAAGTGCTTCTTTTGATCCTGTTGAAGCTCCTGATGGTACTAATGCTTCAGAATATGCAAGTTCAGTATTTAATTCAACTTTAACTGTTGGATTTCCTCTACTATCTAAAACTTCATAGGCATTAATCTTAATAATTTTTGACATAATTCTCCTATATGATTATTTAAAATAAAAGCTAAAAATAAATGAACATCATTTATGTTAGTTTACATAACAATTATATTATTAATTTTTAAAGGTAAAAAAATAATTGTTATTTTGTATGCTTGATCTAAAAGAAACATAATTTTAAAAATATTTTTATCTAATGAAAATTGCTATTTTAAATTAGTGTAAAATTTTATTTATGAATAACATTGATAATAATTTATTTTTTATTGAAGAAGAAATTAAAAAGATTGAAAAAATTGCAAAAGAAGATCCACTTAAGGCAATTTATATTTTAGATGAAGATAGAAAGCAATTCACCCTAAAAGAAGACTTGGATGATTTAGATGCATTGAGGGATAACATTGAATTTCAACTTAAGAAACAAAATTTAATAACCTCAACAAAACTTGATACATTAAGTTTAATAAACAGCTTAAAAAACAAGAAAATGGGTTATGCATTTATGTTGAGCTATAACGAACTTAAAAAGCGAGACGATATAGCAAAATATGCAATTGAATTTCAAGATTTTTTTAAAGGAAATGATTTTGATAGTAAAGGATTTCAAACATTGATTTATGACTTATTAGTCAATAGTAATATTGATTTTGAATACGATATTCAAAACAGAAAAATTAATCCCAAAAAATTAGGATCATTATTAAAGAACGTTGAAATTCAAAAGATGCAAGACGAGATTATTGCAACTTTTGATAAAGATATTTCAAAAAACAAGGTTGCAAGACAAGTGTTTTCAGCATATTTATTTCAAAATTGAGTTGCAATTTTATTGCATGAAACAAAAGACGATTATTTAAAAATATCACACGTAATTGAAGTAATTTTAGGTAATCAAACATCAGATTCACTTTCTGAAGAAGAAAAAGAGTTATATAACATTTTTAAATAAATTATTATGTATAATTTATAACATTAAACTAAAGGAGCATTTATGTCAAGAACATTAAATAAAAAAGAATCAGAATTAACTATTGAATATACAATTCAAGGTGAACAATGAGAAAATGCTAAAGAAAAAGCAAGAAAAGCATTAAGAGCCAATGTTCAAATCGATGGTTTTAGAAAAGGTCACGTTCCCGCTTTTGAAGCTGACAAAAGAATAAATCCAATTGAAGTTTATCAAAAAGCATTGGATAGTATTTTAGATAATATTTATAAAGAACAAATAATTAGCCAAATAACCAAAGATGACGAAATTGTTGGACATGCAAATTTAAGAATTAAAGATATAAATGAAGAAAAAGCAGAAATTTCATTTGTATTTCCACTTTTCCCAGAAGTTAAATTGGGAGATTATAAAAAATTAGGCGTTAAATTAGGATCATGTGAATTAACTGAAAAAGATTTAAATGATGCAAAATCACAAATAGCTTCAAACTATGTAGTTATGCTAGATTCTAAAGAACCAATTAAAGAAAAAGATCAAGTAAACTTTAATTTCAAAGGCTTTATTGACGGAAAACCATTTGATGGCGGAGAAGCAGAAAACTTTGATTTAGTAATTGGATCTAAACAATTTATTCCAGGCTTTGAAGACAAAATGATTGGCCTAAAAGCAGGAGAAGAAAAAGATTTAGATTTAGTATTTCCAGAAGATTATCATGCTAAGAATTTAGCAGGAAAATCTGTTGTTTTCCATGTAAAAATAAATTTTGTTAAAACTGCAAATTATCCAACAATCGATGATAATTTTGTAAAAGAAATTAATTTGCCAAATGTTAAAAATGTTGCTGAATTTGAAGAATATGTAAAATTTATTGCTTTGAAGAACAAATTAGCAAATGTTAAAAATGAATTTATTAACGAAGCAGTTAAAAAATTATCAGAACAATCAGAAGTTTCTTTATCAGAATCATTAGTTGAAGAAGAAGCAGATAAATACTATCAAAATTTCCTTAACAATTTAAAACAACAACAAATTACTGAAAAAGATTATTACGAATTTTCAAAGAGTACAAAAGAAGAATCATTAAATGTATTTAAAAAACAAGCCCAAATAAACTTAAAGCAAATGTTTGTATTAGGTGCTATTGCCAAAGCTGAATCTTTAAAAGTAACATTAGAAGATTATGAAAAAGAAGTTGAAAAATTGGCAAAAACATACGGCTTAGCTCCTGAACATGTAAAACAAATTTTGAAATTTGAAAATGTTGAAAATAATTTAATTAATGAAAGAATTAGCGAAATTTTATTAAAAGAAAATGACACTAAAGCATTTGAAAACTTTGACAAACTATTAAAAGAAGTCGAAGAATATGAAGCTAAACAAACTCAAGCTATTGTAGATGAAGCTAAACGCAAAAGAGAAGAAGCAGAAAAAATAGCTAAAGAATCAAAAGAAAACATTGACGAACAAAAATAATATAATTTAAAAAGTGCCATAAAGATTGGCATTTTTTATTTATTATTTTTAACAATCATTAAAAATAAAATAATATTGTAAAATATTAACTATGAAAATATTTGAATTTAAATCAACAGAAATGAGAATTGCAGAGGCAACCCTTAAGAGGATAAATGCTTTAGAACCAGAAATATCAAAATTAAGCAACGAGGAATTGCAACAAAAAACTATTGAGTTTAAGTCGCGTTTAAAGGCAGGAGAAACGCCAGAACAAATTAGACCTGAAACGTTTGCTGTTTGTAGAGAAGCAACAAAACGTATTTTAGGCAAAAGACCATTTGATGTTCAAATGATAGGCGGAATAATTTTAGATTTAGGTTCTGTAGCAGAAATGAAAACCGGTGAAGGTAAAACTATTACTTCTATAGCACCAATTTATTTAAACGCAATTACTGGTCAATCTGTTATTGTTTCAACAGTTAATGAATATCTAGCAGAACGTGATGCTCAAGAAATGGGGCAAGTTTTTAACTTTTTAGGTTTAAGTGTTGGGATAAACAAATCTCAAATGTCAAGCGAGTTAAAAAGAGAAGCATATGCTTGCGATATTGTTTATTCTGTTCATTCTGAATTGGGTTTTGACTATTTACGTGACAATATGGCAATGAGTAAAGAAGAAAAAGTTCAACGTGGGCTTGATTTTATTTTACTAGATGAAGTAGATTCAATATTGATTGATGAAGCAAAAACTCCTTTAATTATTTCAGGTGGGGATAATGACGAAAGCAATTTGTATACAATTGCAGATTTGTTTGTAAGAACATTAAGTCAAGATGATTATTTTATTGATGAAGAAACAAAATCTGTTTACCTAACAGATAAAGGAATTGAAAAAGCTAATAAATATTTCAATTTTAAAAATCTTTATGATATAGAAAATTCTGAACTTGTTCATAGAATTCAAAATGCTTTAAGAGCACATAAAGTAATGAAATTAGATGTTGAATATATTGTTAGAAACGACAAAATTGAATTAGTTGATTCCTTCACAGGTAGAATAATGGAAGGAAGAGCTTATTCAGAAGGATTACAACAAGCAATTCAAGCAAAAGAACGTGTTGAAATTGAATCAGAAACCAAAACATTAGCAACAATCACTTATCAAAATTTTTTCCGTTTATTTAAAAAAATAAGCGGTATGACAGGAACAGCAAAAACAGAAGAAAAAGAATTTATTGATATTTATAATATGAGGGTCAATGTTGTTCCAACAAATAAACCAATAGCTAGATTTGATGATAAAGATGAAATCTATGTTGATATGCATAGCAAATGAAAGGCAGTTACCGAAGAAGTTAAACGTGTTTATAAAAAGAAACAACCAATATTAATAGGAACTGCTCAAGTTGAAGATTCTGAAATATTGCATGAATATTTGCTATCTGAAGGCATACCTCATACAGTTTTAAATGCAAAGCAAGATGCTTCGGAAGCAGAAATTATTTCTAAAGCAGGACAAGTAGGAGCAGTTACTATTGCCACAAACATGGCTGGACGTGGAACAGATATCAAGCCTTCAAAAGAAGCACTAGAATTAGGTGGATTGTATGTTTTAGGAACTGAAAAAGCTGAATCGAGAAGAATTGATAATCAACTAAAAGGACGTTCTGGTCGTCAAGGAGATGTAGGATATACAAAATTCTTTTTATCATTAGATGACCAATTGATTTTGCGTTTTTCAGTTCAAGATCACTGAAAAGAAATTTTTAAAGAATATGGTGAAGATCCAATTCCTGGCGAAGCAATTAGAAAGGCCTTTTTGAGGGCTCAAAAAAAGATTGAAGGGTTTAACTTTGATAATAGAAAAAGCGTCCTAAATTTCGATGACGTAATTAGACAACAACGTGATCTAATTTATGAACAACGTGATCTAATTTTAAATAGAGATGATCTTGGAACAATAATTAGAAAAATGATATCAGTTGCAGCAGAAAAAACAGTAAATAACCCATTCTTTATAAAAAACAATAGTTTGGATTTAATAGAATTTGTAAAGCATCTAAATAACAATTTTATGATTTTAACAAACAAGGAATTTAGTGTTGAAGAATTAGTTGAATATGATCGTGAAGAATTAATTCAATACTTAATTGAAACTTGAAATAAATGTTATGATCAACTAAGACAAAATATCATTGATAAATATGGGATATCTTCATTAATAAATTCTGAAAGAAATATAATATTAAGCGTTTTTGATGCTGCATGACAAGATCATATTAATATAATGGACAGATTAAGAAGAAGTACAAATTTAGTTCAATATTCTCAAAAGAACCCGTATCAAGTTTACACTCAATTGGGTTCAAAAAAATTCAAAGAATTAACAGACAGAATTGCTTTGGAATGTACAATTAATTTAATGAATAATTACGACGCAATACCTTCAACAAATACTGATGTTGATTTATCATGGTTAACAAATGCTTTTGAAGAAAAAACAGATATGGCTCAAACAACTTCAAAGAACCAACAAGCTCTTGAATTTATCAATTATTTATTGAAGTCAGAACGTGATCATTTGATTAAAGAAGGTTTAGGCGAACAAGAAGTGGATGATAAACTAAAGGAAGTGGAAAGATCAATATTAAAAGAGTTCCAATTCCAACTTGCTTCTTTGCAAACTAATGAAGAAAAAAAATAACGAGTGTTTAAAACTCGTTATTTTTTGTTATATATTCGCAATAAAATTCAACAGCCTTTTTCAATATTTCACAATCACTTTCGTATGTTATGCGATATAACAATGCATTTGTTGTATCTTGCCATTCAACTACCTTAATTTCACTATCTTGCTTAATTAAATCAAAAGATGTTGGCTTAGCAATAAAATAAACAACTTCTTTAGATATTAATTTTCCAATAATATATTTATTGACTTCTCTAAAACCCTTGAGCAATTTTATATCTATGTTAGTTTCTTCTTTTACTTCTCTAATCGCGGTTTCCTCTTCTGTTTCGTTTTTTTCAACATGGCCTTTTGGGAACCCTCAATGGCCTGCTGTTTGTTCAACTAATAAAACAAAAAGATTATCATTTTCTTCTTTGAAAATAATAGCGCCACACGACTTCTCTTTTTTCATTAGCGAAATTTCCCTTACCTTTCTTGACTACAATTTATTTATATAAATTATATAACAATAAAGAAAATGAGCGCCTTGCCAAAAGGTTAATTATTCATCTTTTTAAAAATAAAACTACATAATATTAATGATGAAGCAATTGCAACTAAAAAAATCGCACTTGAAATTCACATTTCAACTTTAATTACTTTACCTTGTCAAAGTATACTTTGTAATAAATTATTATTTTTAGTCATTACTGCTTGAATGTATGTTTTTCTAATTGAAGGTAAAATCAAACCATTAACAATAAAACAAATTCCACATATCAAAATAAAAATGCTGATTATTAATAGAGTCTTTAATATCTTTTTGTTTGCAATTTCTGATTTAAATCTATATGCTAAAAAGAAATTTAAAATATAATAAACAACATAAAAACCGCAAATTATTTGTCCAAAGCAAGATATTGCAAGACGGCTATTATTTGAAAGTGATTGTATTGAAAATATCCCGGTAATCAAAAAAGAAATCAAAATCGCTAAAAAGCCAAATGCGGTTGTTATTAATAATCTTTTTATATTATTTTTTATAATATTGTTAATATCCATAATGCCTCTTTTGAAATATTATAAATGATAATAATAACCTTATTTTTGAATTTGGTAAAATTAAATTATTAAAAGGAGAAAATGTGGCACTTACTAATAAATATTTAGCATTGTATAGAAAATATAGACCAAAAATTTTTGATGATATAAAAGGCCAAGATCACATTGTAACAACATTGAAAAATATTATTTTGAATAATAAATTGAGTCATGCGTATTTATTTTGTGGTCCCCATGGAAATGGAAAAACTTCAACTGCTAAAGTTTTTGCAAACACAATTAATTGTTTACATAATCAAAGCGATATATTAAAACCATGTGAAGAATGTATCAAAAATGTCGATAGAAATCTAGATATTATAGAAATAGATGCTGCTTCTAATACCGGCATTGATGACATTAGAGAATTAAAAGAAAAAATAAAACATTTACCAACACAAAGCAAATATAAAATTTATATTATTGATGAAGTTCATATGCTTTCAAAAGCAGCCTTTAACGCTTTATTAAAAACAATTGAAGATCCACCTAGTCACATAATTTTTATTCTAGCAACAACTGATCCCCAAAAAATACCAGAAACTATTTTAAGTAGGGTTCAAAGATTCAATTTTAAACTTATGACAATTGAAACATTAGTTTCTCAATTGAAGGATATTTTCAATAAAGAAAATATTGAATATGAAGATGATGCAATTAATGTTATAGCTAAATTAGGAAATGGTAGCCTTAGAGATTCATTGAGTATTGCAGACCATGTTGCAATATATTGTGCCAATAAAGTTATTAAGAAAGAAGTTGTTGAACAATTATTTGGAATTGTAAATATAGATAATAAAATAGAGTTATTAAATTGTATGTCAATGCATGATATCAATAAAACATTGAATCTTGCAAGAGAATTATTTAGTCAAGGTATTGATATAAACATCTTGATAAACGATTTAATAAACATTTTAAAAGAATGAATAATTTTTAATAAAACAAGAGATCAAAGTCTTGTTTCAACTGATTTTAAAATTTTATTGCAAATTAAAATTGATGATGCTAAAGCACACTATTACATTGAAACATTGATAGATCAATTGCATAGTATTGCCGTAAGTGATAGTCCAGAAGATTATTTTGAAATTCTGTTGCTAAAGTTATGTAATTACTTAAGTCCAAAAGAAGAATACAAAAATTCTGCAGTAATGCCAAAAATTAGCAATGAAGAATTTAATAATTTTAATAGTCAAGAAAAACAGACCATTAATAAAAATAATATTTACAAATCATTGGATTTATCCTCAATAGCAAATGGATTCAATGCAAACAATTCCGCAATACAAAATTCAAGTGATAATAATTCAACAGAAGACTTAGCTAATAATTCAAAAAAGATTGATGACTTATTAGAAAAAACTGCAGAAATATATTCATTAGATCAGACATCAGAGTTTGAAGTTGCTGATATTTCTAATCAAATAATAAGTAATAATCAAATTAGCATAGATTCAAATGTTGATAACCAAGAAACATCCGATTTACAAATATCGGAAAATTGAAAAGATTTAGTAAATGAGGATTTAATTTCAAAAGAAAATTCAGAAAATAAACATGTTCCTTTGGAAACAATAATCGACTGTTTATTAATAAAACAATATTTGAAAAACAATATGTTAAATGATGGTCGTAGTAATTTTACAGACATTGATGCTATTAAATATGGTATGTTAAGTCAAAAAATAACAGACAAAAATGAAATTAATATCTTATCAGACTTTAAAATTATTTTTTCAACGAATGATTTTATTTTGTTAACGTGTGATATAGATGAAAAAGTCTACGATTTAAATATAAATTCACATAATGAAAATATTATGGCTGCATTGAATAAAGTTTTTGATAGATATCTTTATGTTTATGCCATTACAAAAGATGAGATATTAGAAGCAAAGAAATATTGAAAGGCAAATATAAACGAAATAAGAGCCAAAAAAGTTAAACCTTTGGAAGATTTAAATTTAAAATACAATAAAACAGTTCAAAAAGATGTTGCTTGAGCGCAAGAAATTTTTGGAGATAAGTTCAAGTTGAACAAATAAAAATTAAATAAGGAGAGAGTAATATGAGTATGAACATGAATGAAATGCTTAAAAAAGCACGTAGAATTCAAACTGAAATGGAAAAAGATGAACAAGAAATTCAAAAGAAAGAATTTACTATTGAAAAGCAAGGTATAACCGTTGTTATGAATGGTTTGAGAAAATTAGTGTCTATAAAATTTAATCCTGCATTAATCGATCCAGATGATCCTGAATTATTAGAAGATTTATTAATGCTTGCGGTTAATGAAGCAATAGAAACAGTTGATAAGGCTTTTGATGAAATTAATGCTAAATATTCTGATAGTGGCCTTTCATTCTAATGAACAATATTGAGATACAAAAACTAGAAGAACTTTTAAAAACGGTTTTTGATTTTACTAAAAAACAATCTCAAAAATTTATATTCAACATTTTTAAATCATCAAGTGAAGATATAGATCAAATTTGCAATCAAATAAAAGTATTGAGAAATTCAATAAAGAAGTGTAATATTTGCAATAGATATAACGAAAATACAATTTGCAATATTTGCTTGGACACTAATCGAACAAAACAACTTATGTTAGTTGGCAATTCGTTAGATATTGATAAATTTGAAAATATTGGTTTGTATAAAGGAAAATATTTTGTATTTGATCAAAATATAAGCTTAAAGAATGAAGATTTTATAGTTTCAAGTTTATTAGAAAAATTAATAGATATATTAAAAGATAAGAATGAACTAATTCTTTCACTTTCCCCAACTATTGAGGGTCAATTTAATATGCATTATATTAAAAAATTCTTGAAAAACAAAATAAATTTTACAAATGTTTTTCAATTGTCAACTGGTATTCCTTTGGGCTCATCAGTTGAATATATTGATCAAGATACATTAAAAGAATCATTATTGAATAAAATCAAAATGTAGGAGGAGAAACATTTATGTCAAAGGGAAAATTTGTAGTATTTGAAGGGATGGATGGTTCAGGAAAAACAACTATTATAAAGAAATTAAAAGAATATTTAGAGTCTAAAAATAGACTAGATGATTTCGTATTTACAAGAGAACCAGGAAGCTCACATTCAAAAGAAGCAGAAGAAATAAGACAGCTAATTTTAGATAATGATAATTCTTTTAGTGTTATGGTGGATGCTTTGTTATTTGCAGCAAGTAGAAGGTTAAATCTTGAAAAATGCGTTTGACCAGGATTAGAAAAGAATAAAACGGTTATTTCAGATAGGTATTGAACCTCATCATTTGTTTATCAAGGAGTTTTAGGCGGTTTAGGACTTAAGAAAGTAAAAGATATCAACAATATTGCAACTGAAGAAACAACACCTGATTTAACTATATTCTTTGATATGGAGCCAGAACTATCAGTTGAAAGAATTACGCAAATTCGAAAAACAAAAACTGATAGATTAGAATCTACCAATGTTGAATATTACAAAAAACTAAGAGATTCTTATTGATATGTTATTAATCAAGGTAATGAAAAATATGAAATTATAAAATCTGATTGTAATATTGATGAATTATTTAAAAGAGTATTAAATGTATTAAAAGAAAATAAAATAATTTAATTTGATTTTGTCTTTTTATATTTAAGGTTGTAAAAATATGATAAACAAAAATTTGAGTAAAGTATTGGAAAATTCAATAAAAAACAATAAGCTCCAACAAGTTTATTTACTATTTTCACGTGCAAATCAAGATATTGATTTAGATATTTTAGAATTTGTTAGATTAGTTAATGATGAAAAACAAATAAGCTGGCATCAATGTTTGCAAAAAGAATATTGTTTTATTATTGATGGGGGCCAAGGCGAAATATCGAAAGAAAATTTACAGGAATTTTTAAATCGTGTTTCCAAAAGCTCTCTATTTGCTAATAAGTATAAATTTTTAATTATTAAGTGTGTTGAAAATATTACACTAAAGGCCGCAAATAGTATTTTGAAATTTTTAGAAAATCCTCCATTTAATACAATAATATTATTACATACAATAAAGCCTCAAAACGTTTTGAAAACTATCAAATCAAGATGTTTTATTATTGATATTCGTGATGATTTTAATGCTGTAGCTAGCGTTGAAAGTTTTTTTATTACATATAAAATTGACGTAAGCAAGCAAAAACAATTTAGGGAACTTGCTGATTTAATATACAATTCACCAGCAAATCTATTTAATCTTATTTTGTTTTATATTCAACAATTAAATATAGAAAATTATAAAAATATTATTCATTTTTCTATTTTTATTTTTAAACAAATTTATGAATTAAAAAATAGTATAAAAAAGGATAAAAATATTAGTAAATTTGATTCTATTTCAATATTAAATATTATTAATAATTTATATAATTTTGTTGAATTAATTGATAACAATAATTGTATTTTCAATATTCAAAAAGCAAAGTTAATTTTAAAATTAAAGGAAGAATATGAATAATAAAATTTATATAGTTGGAACTCCAATTGGAAATTTAGAAGATATAACATATAGAGCAATTAAGGTTTTACAATCCAGCGATATAATTTTGTGTGAAGATATAAGAGTTAGTCAAAAATTATTGCAACATTACGAAATTAATAACAAACGCCTAATTGCATACCATAAATTCAATGAAAAAGAAATGACGCCTAAAATTATTGAATTATTGCAACAGGGGATGATTATTTCATTGATTTCTGATGCCGGCATGCCTTGCATTTCTGATCCTGGTTTTGAAGTTATTAAGGCAGCCAAGGCAGCAAATATTGGCATTGATGTAATTGGAGGCCCAACTGCTCTGATACATGCAATAATAAAGGCTAATTTGGATAATTGCTTCACATTTCTAGGCTTTTTAAAAGATAAGTCAGGTGCAAGACAAAATCAATTAAAATCTTTGACATTTGGCACATATGTTGCTTACGTTTCACCTCATAAATTGCTTTCAACTATTAAAGATTTTGAGATTATTTTTCAAGAGCAAGTTAAATTATTTTTAATTAAAGAAATGACAAAAATGTTTGAAACCTCATTTGAAGGAACACCTCAAGAAGTTCTAATGCAACTAAGCGAAGAAGATCAACGCGGTGAATATACTCTTGTATTTAGTTTCAAAGAACCAAAGAAACTTAAAATTAACAAATATAAAAAAGAAATAAAATAAAAAAAGTTCGGACGCGAACTTTTTATTATTTAATTTTTTAAATTTAGTTGTTATTTTTAGCAATAAATGCATCTAAACGGCTAGCTTTTCTAGAACCATTGTTTTGATGTAAAACGCCCTTTGAAACAGCTTTGTCAATTTCATGGTGAGCATTAGCTTGAAATTCAGCAGCTTTAGGATCTTTATTAACAGCAGCAATTTTTGCTTTCTTAATAGCTGTTTTAACTGATGATTTAACGGCTGAATTACGGATGTTTGCTTTTTCGTTAGATAATATTGCTTTTTGTTTTGATTTAATGTTTGCCATTTTTTGCCTTTCATGCTTAATAATTAGCAATAGTATTTTACAATAAAAAAGCGTTTTATTTAGAAAAATAATATTTTTATTAAAATATTCTATATGGTAACAAAGAAAAAATATGATGGATATCTTAAATATTCAAAAAATAATGAATCTGAGTTGATAAATTATTATCTAACTAATAATAAAAATATCTATTTAAAGTTGAACAAGGAACAAGAAATCGTAGCCTATGTGCCACGTAAGTATATGAATACAATAATATTGGATAATTTTTTAAAATCATCAGTTCCTAAACTTCTAGAAATAAAAACAAAAATTGAGCAAAAGAAAATACTTGCAATTGATATAGAAAAAAATAAATTTTATTTATGAGGCAAGGAATCAAAATTTTTAATTCAAAAAAATATATTAAATTTATTTGAATTAAATAAGAATTTAAAAATCTTAAAACAAGGTATAGAATTTACTCTAAATAAATATTTAAAAAAAATATTACTGGAACTATCAACTTTATATGTAGAAAAATTTTGTAAATTATTATGCATACCATCTGAGTCAATAAAAGTAAGAATTTTGAAGAAAAATAATGCTTGGGGAACAAATCATTTTTTAAAAAGAACAATAACTTTTTCAGATCGATTGATTTTTTATAGAAAAGAAGTTTTGGAATACGTTGCTTGTCATGAAGTTGTTCATTTTATTTATCAAAATCATTCAGTTGAATTTTGAAATATGGTAAAAAAATATATTCCTAACTTTAAACAAATAAAAAAAGAATTAAAAGATAAAATTTTTATATAAGCAGGAGATTTCATGGAAAATAAAACAAAATTTATTAATGGAATTATTATAGGATTAGTTTTGCTATTAGTAATAGTTATAATTCTAATTTCAGTTTCAATTGTTCTTTCTAAAAAGAATAGAAGATTTTTAAAAGATCAAAAGGGATTTTTAGATTTATACAATACACAATTAAAAAATATTAGTGAATCTATAAAAAATTGCAAATTTATAGGTGCTAACGATTATTCTTATAACGATCAAAGAATACACTTAGATGATATATTTGTTACTGATAAAATTATTTTTATATTAAAGCCTTATATGTATGACTCCGACTCTTCTATTCAAGGTGACTATTTTAGCAACGAATGAATTTTAACTAAATCAAATGGTAAAAAATCTAAGATACCCAATCAATTAATTAGTTTAAAAAAATCTATGAATAATTTTGGACTATTGTTTTCTAATGAAATGAGTATATGTTCAATTTTTGTACATAATTTAAGCACGGAACTAAATATAGAAGATTTGCCATCGTTTCAAGGAATTTGCAATGTTAAAGATTTGTGTGCTTCAATAAAATCTGTATATAATTCGAATATTAATTCAATGTATAACCAATCTGTTTCAACTATTTTCAATGTTTTAAATAATAAAATTGCAAAATAATTGTAAAAATTTAGTATTTTCAATAATTTTTCAATAAAACAACAATATCTTTTTGCTATTTTAGTGTTTTTGTGTAATAATTTAATTGTAAATATTAATATTTAATTTATTTATAAAATTTTTTTAAAAAAATAACCTTACGTAAGGAGTATCTTAAATATGGCAAAAGAACAAAAAACAGTATTGGAAAAATACTATATTTCTGAAGCTTTTGATAAAGATAGAAATATTTCATTTAACTTTAAAAAGGCAAATAAGGGCATTTCAGGAAACTTTTTAAATTTTTCTAGCGCAGTTGAAGAATTTATTAAAGTTAGTGAAAATACACAAAACCCAACCAAATGTTGATTCCACCGTGATGGCGCTTACCGCGGATGCGTAAACATTGATGGTGCCCGTGTTATTGTTGAAAAAGTTGCTGAAAAACAAGTTAAAAATGAAGAAGCTATTGAATTCATCGAAAGAGAACAATTGGTTGAAAAACCTGCATCAAAAGCTAAGAAAGTTGAAGAACCAAAACAAGAATGTGAATGCTCATGCTCTTGTGATTGTTTAACTGGTATAGAAAACAATAAACATTGAAAAGAATATTGAACAAAGAAATCTTCAAATAATATTGTTTGTTCAGCTACACAAGACCACAAAGGCGGAGAAAAACTATTTATTGGTCTAACAGTGTGTTTATCGATTCTAACAATTATTAATTTGATTTTGATTATTTTGTTAGCAACAAACGTTATTTAATCCAATAAATTAAAAAATAAAAACTGGAATTCCAGTTTTTATTTTTTAATTTATTTTAAATTCTTTTTTAATTGTTTTAAGTAATTTACAAATTCTTCTTTGCTGTATGTTTTAGAATCATCTTTTCCATATTCTCTAACTGATAAGGTATTTGTTTCAATTTCTTTTTTTCCAATTACTACTATAAATTTGGTCTTTAAAATTTGAGCATCACGAATCTTTTTATTTATTCTTTCTGGTCTTATATCAATATTAACATTAAAATCTTCATCTAAAAATTCGTTATATAGGTCTTGAGCTTTTTCATTTAATTCATCATTAATAGGAATAATTGTTATTTGTCTTGGACTTAATCAAAATGGCAATACACCTTTTGTTTGTTCAAGTAGTGTTGCAATAAATCTTTCATAAGTTCCAATTAAACCTCTATGAATTAATACTGGTGTTACAAATTGTTCATTGCTATCAACAAATTTCATTTCGAACCTTGAAGGCAATAAGAAGTCTAATTGTAAGGTTGACATAGTAATAATTCTATTTAGTGCAGTTTTTACTTGAAAATCAACCTTAGGTCCATAAAATGCCGCTTCACCAATAAATTCTTTATATTTAATATTTAATTCACCTAAAACTTCACGCAAGTCATTTTCTGCTTTATTTCACATTTCATCATTTTGGAAGAATTTTTGAGTATCTTGTGGATCTCTTAATGATAAAGAAATATGGTCTATTTCGATATTAAAATCTTTCAATGCTTGTAATATCATTTTATACAAGTGCTTAAATTCTGATCTTATTTGATCTGGTCTAACAAAAATGTGACCTTCTGTTAGATCCATTCCTCTAACTCTTTCAAGTCCGCTTAAAGCACCCGATGCTTCGTAACGATATAACCTTGATTGTTCTGAATATCTAATTGGCAAGTCTTTGTATGAACGTCTTGTTGAATTGAACAATATTATATGGTGTGGGCAAGTCATAGGTCTAGCAACTAATGTTTCGTTATCCATTTTAATTGGGGCAAACATAGTATCTTGGTAATGTGCTCAGTGTCCACTTATTTCATAAAGCTTCTTTTCGCCAAAGTGTGGAGTTAGTACTTCTCTAAAACCAAATTTTTTATCAAGTTTTAAAACATAATCTCTAATTGCATTATGAATCTTCATTCCGTCTTCTAATCAAAATGGGAATCCTTGTCCACCTAATTGGTTAAAAGTGAATAAATTTAAATCTCTTCCTATTTTACGATGATCTCTTTCTTTTCTTTCCCTTAAGATATCTAGATATTTTGCTAATTCATCTTTTGTTTCCCAACAAGTACCATAGATTCTTGTTAACATCTTGTTTTTAGAATCGCCTCTTCAATAAGCACCGGCAATTCCTAACAATTTGAAGTATTTAATTTCTCTTGAATTTTCAATATGGCCACCTGCACATAAGTCTGTAAATAGAATTTCTTTTGTTTTTGGATTTCAATATTGAAAGAATGTTATTTCTTTTCCTTCTTTTTTAAATTCTTCGTAAAGTTCTTTTTTATATGGTTGATTTTCAAAAGAATATTGTTCAATGCCAACTTTTCGCATTTCGTAACCTTCTTTTGCTAATTCTTTCATTACTTTTTCAATATTTGGAAGATCTGATTCTAAAATTGGATTTTCAAATTCAAAGTCGTAATAGAACCCTTCTTCAATGGCTGGTCCAATTGCCAATTTAGCATTTGGATAAAGTTTTAATATAGCAGCCGCTAATAAATGGCTTGCTGAATGATTTAATTTTTTATTTGCTTCCATTATCTTAATCCTATTCCTTTTAATAACATATCTAAGTTAATTTGGGCAACTTCACTTGCCTTTTTTGCTCCCTCTTTGCTCAATGTTTCAATTTGATTTAAGGCATTTTTATACTTATCTTGAATCTTGATTAAAAATTCCTTTACACATTGAGCGACAGCATTTTTAAATTCACCGTAATTGCTGTTTTTAAATTTTTCAGCGCTTTCTTGTAATGAAATATCATTTAAAGCACTATATATAGTCAAAAGGTTTTTTATGCCCGGCTTTTCATCTGACAAATATATTTTTCCTTCTGAATCTGTAACAGCTTTTAATATTTTTTTGTATGCATCTTCTGGATCATCTAATAAATAAATTGCTGATTTAGGATTTGTATCAGATTTTGACATTTTCTTACTTGGGTCAGTTAAAGACATTATTTTGGCCCCAACTTCTGGTATATATGGTTCAGGTATTTTGTACGTTGTATTGAATTTATTATTTAATCTAATTGCTAAATTTCTAGTCAATTCAACATGTTGCTTTTGGTCAATGCCAACAGGAATAATATCTGCATTATATAGTAATATATCGCCAACCATTAAGGTAGGATACATTAAAATTCCTGTTGGAATTGTTTCCATACCATTTGCAGTTTTTATTTTAGCTGATTTATCTTTAAATTGCGTCATTCTTGATAATTCTCCGATTGTCGTATTTGTCAAAATTAATCAATTCATTAATCCGTGTTGAATGACATCTGATTGAAAAAATAACACACTCTTTTTAGGGTCAATTCCACATGCAATATAAAGTGCAAAAATATCCTTTTTATTCTTTGATAATTGCTTAGGATCAATTGGCAACGTTAGAGCATGTAAATCTGCTATAAAAATGTAATTGTTATATTCTTCTTGCAATTTAACCATATTTTTTATAGCGCCAATATAATTTGCTAGCGTTAATTTACCAGTTGCAGTAATTCCACTTAATAAAGTCTTCTTTTCAGAATTTTGCATAATTAGCCTTTCTTTTATTGTTTAATACAAATAAAATTTTATAACTTTCTAAGATTATAAAATAATTTTATTTTACATTTATAAATTAAATAATATTAATTAATATTAATATATTTTTATTAAAATATAATTATATTGCTTGCATAAGTTACATAGGCAAAACTATAATAGGAGAAACAAATGCTAAACAAAAATAAATTTTTATACATATTTTTAATAATCATTACTCTTGGGTTAATCTTAATATTTTGAAGAAAAAAATACAAGGTTTTAAAAACTAAGAATTATTTAACAACAAACAATGAAATAACCTTTTCAATGCAAGATTTAATTTTGTATTTGGGTGGAATTTCAAATATAGTTTCTTCATCAGCAACTCAAAAAATTGTAAGAATTGCATTTAAAAATAAAACAATTATTTCTATTGAAAAAATAAAAAAACTTAATGGCATATCAGGTATTGCATTTCAATCTTCAAATATATCATTAGTTGTTGGAAATTGTGCAAATTATTTATCTACATTACTTAATAAGGAATTAAACAATCATGAATAACAAAGATTCTAAATATCAAACATTAATGGCTAATTTATTGAATATTGATACATATGATACTTCACTATATACATCTATAAATAGTGAAAATTTTTTTGATATTTATAACAATTTTGGCCAAGAAATATTTGATCAAATTTTCAAAAATTACAAATTTAATTGTATTTTAACCGAAGTCAAAAATAAAGAAATGCGCCAATTGCTAGAAAAGGCACAAAATAAGCAGGAAATAATAACAATTTATAAAGATAATAACGTCAGAATCGAACAATCGATATTCAAGATGCTATCAACTGATTTTGAAAAGGCTAAAACTTTGTTAATTACTAAATTAGAATCAGAATTTCAAAAATCAATAGTAAATTGAAAAAAAATAATAAATAAAGCTGAAAATATAAACATTGAAACAAATATCTGACCCTTGCATGTAGGTTTTTTATTTATTTCAATAAAAACGGATAAAAAGACAATCTTTGCTCCCTTATTATTTAAAGAAGTAACATTAGAAGTTAAAAATTCCTTGGTTTATTTGAAATCAAATTCAGATGTAAGAATAAATAGTAAGTTAGTAACATTTTTATCTCAAGAAGGTTTTTTGTTAAATATTGATAATTTAGATTTTTCTAATTTGTCAATTGAATCTATATTTGAGCATTTAAAAAGAATTTGATCTCCTATATACAATATGCCTCAATCACTAAGAAGCAATATTCCGCTTGTATCTCAAAGTGATATTCAAAACGTATCTATCGAATTTCATCCCGGAATGATTTTTGGATTTTATAACGTTTCTTCTGGATATTTATGAAATCAAATGAAAAAAATTATTGAAAACGATGAATTTGAAAGTATTTTAGTACCCGAAATTAATAAAAATGTTTATAGAGAAAAAATTAATACAAGTATTTTTAATAATAAATTTAAGTTATTTAAGGTACAAAAAACAAACTTTTCACAAGATGTTGCTACAGTGTCTGCACTATGTCAAGATACAATAATTTGAGGACCTCCAGGTACCGGGAAATCTCAAACTATTTCTAATATTATTGTCAATATAATTGCAAGGGGATATACTGCGCTAGTTGTGTCTCAAAAGAAGGCCGCCCTTGATGTATTGAGAAATAGACTAGGACAATTGAGCTTATTTTGTTTATTTGCTTTAAATGATAAGAATTTAAGACAAGAGGTATTTTACGAACCATTAAAAAAATTTATAGAAAAAATTGAGAATTTTTCTATTTCTCAAGTTGAAAAAGGAATTCAAATTTTTTCTGATGAAGATAAAGGTTATGTTGATACATTGAATGAAATAGAAGCATTGCCTAACTTAAATAACTTACTTCATTTCTATGCCTCAACATTGAATGCCAATCTAACAATAAATGATTTTAATACCCTAAGATTATTGGCAAAAGATATAAAATATGATGTTGAAGGACATACTTTTAATGATAAGAAAGAACTTAAAAAATATCTATATGAAGTAAATTTAAAAAGAAGACCGTCAATATTTTCAATTTATCCAAGGTCAATAAAAGAAGCTAGTGAATTAATTATTAATGCGCCTGAATTATTTAAAATCAATGTAGATGAAGCAGTAAAATACATTGATAAAGTAGAGTTTGCCGAATTGGAAACTTTTGTTGAAAAATATAAAAATTTATTGATTAAAAAAACAATAAATGTAAGTAACTCAGCCGTTTTATCTAATATGTTATTTGCTAAATTAATAAATACAATTTCGAATTTCAATGATGAACAAAAACGTGAATATACATCATTTGCAATGTCTATAAGAACAGGTAGCTTAAAGCCATATATGTTTTTCCATAGATATAAAAACATAATTAAAGTATTATTTCCAATAATTGTAACTACTCCTGAATTAGATTTGTCAATGTGAGGTAAAGAAGAATTTGATTATGCAATCCTAGATGAATCAAGCCAAATCTTTATTGAAAAAGGAATTCCAATTTTATACTTAGCTAAAAGAAAGATACTTGCTGGAGATTCAAAGCAAATGCAACCAACACGTTGATTTTCGGTTTCATATAATTTTGAAGATGAAGAAGAATTGGGTGATATTGAATCTCTTCTAGATTACGCTACTGCTAGAGGAACTTATTCAATATTGCTTGATAAGAACTATCGTTCAAAGAGGGCATCATTAATGACATTCAGTTCTAAACATTTTTATGATTCCAAATTAGATGTAATAGATGATTTTAAATTAGCATTTTCTAGTGAAAAACCAATTGATGTAATTCAAGTTGATGGTGAATGAAATAATAGTTACAATGAAGCTGAGGCTAAAGAAATATTAAGAATAGCAAAAGAAAATCTAACAAAATATGAAAAAATAATTATTCTTGTTTTTAATGTTAAACAACAAGAATATTTGACAAATGCAATATTTAGCGTTGAACCAGAACTAGAAAAAGCCTTATTTTCCGATAAAATAACAATAAAAAATATTGAAAACATTCAAGGTGATGAAGCTGATTTAGTCATTATGTCTGTTGTTTATGACAAAAAAACAGCATTGTTTGGAACATACGTTGCAAGGAAGGGTGGAAAAAACGCCCTAAATGTTGCCATATCAAGAGCAAGAGAAAAAATTATTGTTGTAAAATCAATTTATGCAGATGATGTTGAAATTAATGAACGTTCAACATCTGATATGCGTTTATTCAAAGAATGGCTAAAATTTTTAGATTTATCAATAATTGAACAAAAAAACTATTTATTTTTAGATAATGAAAATAAAAAATATGATGAAAAAACACAAACAATATTGTTACCAAAAGTAAGCAACCTTCAAATGGATGTATTGGCAAAATTAAACGAATTATTGAAGGAACTAAATGCTAACAATTTCGAAATAATTTCGAATTATTCAATAGGAACTAAATTATTAGACTTAGTCTTAATCAATAAAATTTCAAATAATATGATTATTGGTATCAATATTGACGATTTTAAATATTTGCCTGATTATAGAAAATATTTAAAATTTAAAGATGATTTTAATTTTTTACTATCAAAATCATATCCAATAATTAATATTTCTAGAATAAGTTGAATAGTAGAAAACAAACAAATTTTAAATAAGATTAAAATTTTAATTAAACACGAAGAAGAGTTAAATTCTCAAATTTTATTGCCCGAAGAAAATAACAATTTAAATGTAGAAGAAATTAAAGAAAACATTATAGAAAATAATGCCTTAAAAAACATAAAATCAAGCCAAATTAAACAAGGTCCTGAATTAAAAGAAATAGATTTTGAAAATGTTGATGACGAAACTTTTGAAGTTGATCAAGATTCTAATAATAAAATTTATGAATTAAATGAATCTGAGGTTGCTCAATTTGAAGAATTCCATAATAAGAATATAAATCATGAATTTAATGACCAAACCGAATCAAGAGCCGAATACTCAAAGGACTATGAAAAAGATTTTTTGATTAATTATAATGATGTTGAATTAGATAATACGCATATTGATCAGGAATTTCACATTGAAAATGAATTTGAATCAACAATAAATAATAGTAATGCGGTAAAGATTGAAGATGCAACTTCATCATCGGCTAACGATGAATTAAAAATAATTAATTCTAATGAACAAAAAATTTCTCCAAAAACAGCAAGTGAAGATATTGAATTGGGATTTTCAATTGAAGATATAAACAAAGAAAATATTAGCAATTTATTTTCTAATACATTTGATGATCTTGATATAAATATAGCACCTGAAAGTGAACAACAAGTTGAGGCTAAACTTAATTATGATTCAGGCATTATTCAAGATAAGTTGCATGAGCAAAACGATGAATATTTTAATTTAGAAGAAAATAATATTGAAGAGCAACCAAAAAATAATAATGTTCAAAGCGCAGATGAGACATTAAAATCTCAATTAGTTTATATTGACAATAATAAAGAAGTAAACGATAAACAAAAAAGCTATGATTTTGCAGGTTTATTTGCTGACGAAAATGAAGAAACTTCTACAGATGTAAAAAACATGATAGACATGAATACTGCAGAATATAATCAATATATGAAAAATAAAGATATTGAACAAGATGAACAAAATAAAGAGATAACTAAAAATACTGCTGATGAAGAAATATTGAATTTTGATTTAATCGAAGATGAAAAAACTACGGAATTCAATGTGGAGGACAACAATGTCAAAAAATAGCGACCCTCTTGATCAATGAGAGCAAGAAATAAAAAGAAGAGTTTCAATAGAAGATCAATTATTGCACAAAGATATTGGAACTAAAATTGCAAAGCAAAAAAAGATTAAAAAAATTTTGTTCATAACTTTTTCTGTTGTTGGTATTGCTACAATCGTAGGGGCAACAATTGGAGTTACAAGCAGAAAAAAAATGGAATTAGTAACTTATGATGATTATGTTGATGTTTCTCTATATTCACAAATAAATTCTAAATTTAAAGAATTGTATCTCGATGGAAAACAACCTGACAAAAAAAATTACCCAATTACTTGAAATGTAAACACTAAACCAATTTTAGGTGAGCCCTTTGAAATTCTAAAAAAAATATTAAGTGAAGGTGCTAATTTAGACAATGAAATTTTTAAAGAATTCAATTTTAAATATGATGCTAGTCAATGAAATCCTAGATTAACTAACTTTTTTTTAGATGCAATAAATAGAAAGCATTTGCATTTTGATATTTATTTAGAAAACAAGAAACATTTAAATCAATCATATGTGGTAAGAAATTTTACTATTGAAACAACGAATGTTTTAGATAATGAAATGTTTTCTGAAACACAAATTGCCAAGTGAAACGAGCAAGATCCTAATATTTCTAAGGCGGCTTTAACTAAAAATTGAAATTTCTATTATGTTACTAAAACAAATGCAAACCTAAATTTTGAAAATGATAAAACATTCGAGGAACTTGGAAACAAAATAAGTAATTTTAATAATAAAATTTTGAATAATAGTGATTACAATTACGTTGATAAATCGCTTTGGTTAACATCTCATCCTAAAAAAACCGATGAATCCGAATTAAATTATTTTAAGTTGTTTCAGTCAGATGTAAGAGACTGGTTTGTTCCTTTTAATTTCTTAAGTCAAGGAATTCAATTGAAAAAGATTGAAAAAAATGAAAATAATATTTTTACTAAGACGCTCAATTTAGAATTCAAGTCAACTTTTTATACTATTGTAAAGAATAAATTAATTTTAAAAAATAAATCTAGTTATGAATATGCTATTTATGTTCCATTTTTTACAAATAAGGATAAGACCATTAAAGACGGCAAGGAAATTTATCAACCTAGAGTTTTTGAATATAGTTGTCCTATAACATTTGTTAATAGTAATTACAAAAAATTTAAATAAAAAACCGTTTAAGTCGGTTTTTTATTTAACTAAATTAAATGAGAATTTTTTTCTTGCTTTCAAGGTTGATTTTTATTGATTCTGTCTATAAATAATTTTCCTTGTAAGTGGTCATATTCGTGTTGCAATACAATTGCCGGATAGCCAGAAACATCATAAATTTTTTCTTTTTTTTCAAAATATGAATATGCTTTAATTACAACTCTTGAATATCTATATACAAAACCTTCTTGACCTGCATCGCTTTCCTTAACACTTAAACATCCCTCCCCTTCTTGCAATGATATTTTATGGTTTGAATGTGAAAGCATTATTGGATTTATTAAAGCATCTCTAAAAATATAATTATTTTGATCGTCTTTTACCATTACATAAAAAATATTTTTCAATATTCCATATTGAACTGCTGCAACTCCAACGGCAGGTCTAAATTTTGTTTGAGGAGTTTGACTATCATCAACATGAAAAATCATTTTTTGTATTAATAAATCATCTTCTGCGGTCAATGGCAGTTCAACGTTCTTTGATTTATTTCTCAATACTTTTTCTGGTAATTGAACTAATTTAACATCTTCAAATTTAAACATAGTTAAATTATTACATATTTAATTGAATTTAAATGAACTTTTTAAAAATATTTTATTATAATTAGAGATTATCTTAAATATAGGAGAAATTAATGCCAGAGCCTTTATCCGGTAACTATAATACTTTAAATTCAGCATCAGGAGCTTCAACTTCAACCGGTGCACCATGACAATACATTTTATATTCAATCATTCTTTTTTTACTAATATTATTTAGTGCTATATTTTCAGCTTCAGAAACAGCATATACAACCGTTTCAAGGGCTAAGATTGAAAATATGATTGAAAAAAAGCAAAAGTTCGCTAAATTAATTCAAAAACAACATTTATTTTTTAATAGAATTTTAGGGACTATTTTAATAGCTAATAATTTGGTAAATATTGCATCTGCAACATTGTTTTCATATGTTTTATCATTAACAACAATGTCTTCTGGCAAGGCTACTATTATTTCAACTGCTGTTATGACTCCACTTATTGTTTTGTTTGCAGAAATTTTACCAAAATTAATTGCAAAAGCATATCCTGAAAGAGTTATTAGATCAACGTGTTGATTTATTGAAGCATTATATTGAATATTTTTTCCAATTACTTATCCAATATCTAAAATTGGCAAAAAGATTTATATAACAAATACAGAAGAAGATATGAAAAACTTGATTAATATTGCACAAGATGAAGGTGTTTTAGAAATCAACGAATCAACTATGGCAAAGAATGTTTTGGATCTTGATTCAAGCAAGGTTTCTCAAAATTACGTTAAGTTAAAAGATATTGATTATTTGAATTATAAAGCAACTTTGCAAGATGCATTAAACAAGTTTAAAGAAACTAATTATTCAAGATTGCCAATTGAAAAAGATGGAGATATGATAGGCATATTGCTTTTAAAAGATATATTCTTTTTAAAGCGTGGCAATATTATGAATTATGTTAAAACGGTGCCAAAAATTTCCGCAAATAGTTTACTTTCTATTGCATTAGAAAAATTAAGGAAGGCTAGAGCACAAATGGGTTTCGTCATTAAAAATAATAATGATGACAGTGTCATAGGAATAATTACAATCGAAGATATTATTGAAGAAGTTGTAGGCGAAATATACGACGAATATGATGATGATGAACAAATTTATGAAATAAGTTTGGAACGCTGTGAGGCCCGCGGAAACGTTAAAATTTCAACAATTTGAAAGCGCTTTGAGTGAGATGAAATTTTAAATTTAGAAGCTCCTAATGATGAGGATTTAAATTTATCAAATTGACTAAAAAACCGTGTTGGCCACGAGCTACATGTTAATACAAAATATACTTACGAAGATAAAATTGATTTTCAAGTGATATCATTAAAAAATAAAGATCAAAAATTTGATATTATCGATATAGATTGAAGTAAATAAAACCTGTTTTTCTTAACGGGTTTTTTATATCTTTATTATTTATTTTATAAATAAACGTTCATTTTATACAAAAAAACTATTTTGTGTATATAATGTAGAAGTTAAAAATATTTTTAACAAGGTTTATGACTCCTTGACCGTTAGGTCCAAATGTCCAAAAGGAGAAAAATTATAATGTCAAATTACGAAATTATGATTCTAACTAATCCAAAAACAACAGATGAAGAATTACAAAATCTAGTATTTTCAGTATTAGATAAAAATTCATCAAAATTTGAAAGATTAGAAAGAAACGAAACTGCATATCCAATTAAAAAGTTAACTCATGTAAATTACTTTTTAATTACAACAAAGGCAGAACCTCATTTAATGTCTGAATTAACAAGAAAGCTTAATATCGATAAATCAGTTTTAAGATCTTTAATTATTAATTTAGATTCTGAAAAAGGTTTAAAGCCAAGAAAAGTTTCTAAGAAATTCAATAAGAGAATTCCTCTAAACCGTGAAAATAAAAAACCTTATGTAAAAAACAACGAAACTTCTAAAGATGCTAGCGAAGAAAAGAAGGTTGCAAGAAAACCAAGAACTTCAAAAGTTTTAGACGAAAAGAAATAGTTAAAAAATAATAAGATCGGTAGGAAAAATGAATAAAGTATTTTTAATTGGTCGTTTGTCGGCCAAACCTTATAAAAATGTTACACCTTCTAATGTTGAATATTCAAGATTTACTATGGCTATTAGACGCGAATACGCCGCCCCTAATTCTGAACCTGTAGTAGACTTTGTCCCATGTGTTGCTTGAAGATCTAACGCAACTTTTATTAATAAATATTTAGATAAAGGTTCGCTTCTTCTTGTTGAGGGTACATTTCAATCTTCACGTTTCAATAACGGCGATGGTCAAATTTCAACATCATATGTTGTTAGTGTAGAAAAAATTCAATCACTAGAAACACGTGAAATGTCAGAAAATAGACGAAAAAATAACAATGGTAAAGAATTTAATATTGCCACAGAAGAATCAAACACTATCGATAATAATTTAGAAAATAATCATCAATCAAATGAATCAAATGATAATCCTTTTGATCAACTTGATTGAGATTTATAGGAGTTAAAATGGCAAGAATTGTTCGTAAGAAACCTTTTATTCGTAAACGTCCATGTCAATTTTGTTTAAGTAAAGAACCAGTTGTTTACATTGACTACAAAAATGAAGAAGTTTTATCAAAATTAGTAAATTTACAAGGAAAAATTTTATCATCACGTATTACCGGAACATGTGCAAAACACCAAAGAGCAGTTGCATTAGCAATTAAAAGAGCAAGATTTGTTGGTATTTTGCCATACATCGGTTTAGTAAAAAGAGAATCTAAAAAAGAAACTGTTAAAACTGAAAAAGTAGAAAAAACAGAAAAAGCAGAATAAAATAATAAAAAAATCAAGTGTATGTATTTGCTATATAACTTGATTTTTTTATTATAATTTTTAGTAATTATGAATATAAAAAGCAAGCAAAAACTAGCAATATTTATTGGCGTTGTCAATTTTTTAACAATCGTAATTTTTTTATGTTGATGTTTAACTTATTTTTTAGAAAAGAAAATAGATTTTAAATTATTTGGTTATATTTGTTTAGGCTTTACATTGGTTGCTTTTTGTGTAAATATAGTTGGATTAATTTACACTACAAAATATGATATTAAAAAACCTTGAAGAGTTTTTTTTATAAGCCTTTTAATAATACCTTTAGGATTTTTTGGGGCAATAAGCTTAGCGTTAAATTATCCAGAAATTAAAAAAAATAGGAGTAAGAATTAATTAATATGACTAAAAAAATAAGTTTTAGCCAGATGGTTTTAAATTTAGTAGCATTTATTTGTTTCATTGTGCTCATTTGATCTATTGTGGCTAATATTTCCTTTAAATTTACATATGGAAAAAATTTTCAAAATCAAATTTCGGTTTATGAAACTAATAAATTTAATATGATCAATAGAGTATTAACGGCTTTTTTAGTATTATACATTTTATTTACTATTGCTTCTATTTCACTGAATATTTATGTTTCAATAATTTTATTCAAAAACAAGCATGCTAAACTCTTTTTACTTTCATTGGCAAGCGTTTTAGTTTCAAGAATAATTGGTGTTTTTTGCGGAATCATTCTGCTTTTAAATAAAAGACTTTCAACAAGCAAATGCTAAAAGTCTTTTATTTTTTAATAAAATAAAAAAATTAAATATTTTATAATTTAACCATATGGATTCTAACTTAGCAAATTTAATAAGGCCGAAAATTTTAGAAGATTTTATTTGCCCACAAGAACAAAAAAAATTATTTCAAAAAATTATAGAAAATAATGATTATCGTTCATTTATTTTTTATGGAAAGCCAGGTACCGGAAAAACAACTATTTCCTATATTCTTGCCAATGCCTTAAAAGTAAATTACGAATATTTTAATGCGGCTATTGAAAATAAAGATGAGCTTGTGCATAAATTAAAGTTGAACAAGATTTTGATCATTGATGAGATTCATAGATTAAACAAAGATAAACAAGATATATTGTTACCATATTTAGAAAACGATTTAATCACAATTTATGCAACTACAACGGAAAATCCATATTTTAAGCTTAATCCTGCATTACGTTCAAGATGTTCAATAATTGAAATTACTAAGCCAACAATTCAAGATTTATCAAGTTGTTTAAAAAATGCCTGCCTTAAAACTTTTGGTGTGGATCTTAATAAGGATATTGCTGATTATTTAGCCCTACAGGCTAATGGCGACTTTAGATCAGCATTAAACAATCTTGATTTGATTATGTTAATTTTTAAAGATCAAGAATTTTCATTGGAGGCAATTAAAAAGATTATTCCCTCAATTCAATTTAGTTCAGATGAATCAGGTGATAATCATTATGATTATTTAAGTGCTTTTCATAAATCACTACGCGGTTCGGACATTGATGCAGCATTATATTGAGGATTTATCATTATTAAATCCGGAGATTTCGATGGCTTGTTTAGAAGAATGTTGTGTGCAAGTTACGAAGATGTTGGAATGGCAAATCCTAAATTGGGATCAGAGGTTTTGTCAGCAATTGAATGTTTTGAAAGAATAGGAATGCCTGAGGGGTATTTACCAATAACACAAGCAATATTAAATATTTGTTTAAGTCCAAAATCAAACACTGTTTATAGCTCATCAACATTAATAAAATCTATAATTGACAGTGGAAATATTTTTGAAGTGCCAATTCATTTAAAAGATGCTCATTATAAATCGGCAGCAAAACTAAAAAGAGGATTAAATTATAAATATCCTCATGATTATAAAAATAATTATATTGATCAACAATATCTACCAAATGAATTAAAAGATAAAATTCATATTGAATTAAATGGGCAAGGTTATGAACAAAAAATTAAACAATACTGAGATTTAATAAAAAACAAAGGAGAAAAATAAAATGGAATTTGATTTAAATAAAATAAATACATTGGAAGATTTAAAGTTAGCTAAAAATGCTTTCAATAATTCAGATGAGTTAAAAGAATTAATGTCTCAAATTAAAACAGCAGATCCATTGCAAAAAGCAGAACTTGGTAAAAAAATTCAACTGCTAAAAAAGCAAGCAGAAGATTTTTTTGATTTAGCAAAGCAAAAACTTGAAGATATAAAAATTCAAGATTTAATTAATCAAGAGTATGTAGATTTTGCAAATCCTATAAATACAACTGGAGGAATTCATCCAATTAATTTAGTTGCAAATAGATTTAGAGAATGATTATTGGCAAATGGATATTTCGAATATACGGGACCCGAAATAGAAAATGAAGAATATAATTTTGAACGTTTGAATATTCCCCAATCTCACCCTGCAAGAGAGATGCAAGATTCACTATATATATCAGAAAAAGAATTGCTTAGAACTCACAATACAGGATTTAGTGCAAGAGCATTAGAGGAACATAAAAATCAAGAGTTTTCACAATTCGCTATTGGGAAAGTTTACAGAAATGATGAAGAAGATAGAACACATACTCACCAATTTACTCAATTAGATTTTGTTAGTGTTGGTAACCATTCATTTGGAACATTAATTTATACATTGACTGAATTATTTTCTTATGTTTTAGAACAAAAGGTAAAAATTAGACTACGTCCTTCTTATTTTCCATTTACTGAACCTAGTGTTGAAGTTGATGTTTTGTTTAATGGAAAATGAGTTGAAGTTTTGGGTGCCGGTATGTTGCATGAAAACGTTTTAAAAATGGCCGGATATACCAATGATATGAATGGCATTGCTGCAGGAATTGGTATTGAAAGAATAACAATGATAAAGTATGGCATTGACGATATAAGAGAATTGTATGGAAATGATCAAAGATTTTTAAAACAATTTAAATAAAAGGAGCAAAAATGTTATTTTCTTATAAAAAAATAGTTAAATTGGCAAATATAACTGACAAAAGTGTAGAAGAGGTTGTTGGGGCAATTAATTCAATAGGCTTTGAAGTTGAAGAATATCATAAGTTTCTAGATGCCGAAGGTATGAAATTCTGTCATGTATTAAAGGCATACAAAAATCCTAATTCCGATCGTTTAACAGTTTGTGAGGTTGAATTTGGTAATGGAAATCATTCAATAATTCAAACCAATGCAACAAACATGCATGAAGGCGATTATGTAATGGCTTATGTTCCTGGATCAAGATCTGGAAAAAATGTATTTGCTGCCAAGGAACTAAAAGGAATCATCTCTGAAGGAATGTTTGTAAGTTTGAATGAAATTGGTATTCCAGAAGAATTTCATCCTTTAGATTCAAAAGATGGTATTTTTCAAGTTGGAAAAATAGATTTAAATTTAGACCCTGTTCAATATTTTGATCTTGATGATTATTTGATTGATGTAACGATTTTATCTAATAGAGCTGATGCATTGTGCTATTTAATTTTAGCTAAAGAAATTTCAGCATATTTTGAATCATATGTTGAACCTCTTTCAAAACCAAAACCTACTTTGGAAAGCGATATTGTTGTAAGTAAAAATTTAGTTGCTACAAACTGTTTTAGTTTAGTTGAAGCAACTACTAAACAACCTACTATTTCTCTTCAAGATGAATTTCTTTTATGAAAGCATGGAATAAAAACATTTAGAAATGCAGTTGATATAACTAATTTGGTCTTATTATATGCGGGAGTTCCTTGTCATGTATATAATAAAAATTCTTTAAGTTCAAATGAGTTTTCTACATCATTGAGCAATGAAACATTAAATGTCTTAGGCAATAAACAAGTTACATTAAACAACAATTTGGTTGTAAAAAATGGAGATCAAACTGTTTCAATAGCAAGTGTTATAGGACTAGAAAATTATGAATATAGACCCGATGGTGGACATTGCGTCTTTGAATTAGCATCATTTTCGTTAAAAGAAGTTAGAAGAAGTATAAAAACAGTCAAATTAGAAACATTGGCATCTTTACGGGGCTCAAAAGAAATATCATGTGGTCAAGTTGTTATGGCATATGAATTTTTAACTAAATATTTAGATCAATATTCAGTTCAAATAAACGCACCAAAGCAAAGAAAAACAAATATATTAATTGATAGTCATTATATTACTAAATATGCGGGCTTTAATATTACAAAAACCAAAAAATATATTCAAGTCTTAAATCAATTAACTCTTTTGGGCTTTAAAATAAAGGAAGATTTATCATCAGTAATTTTTCCAACTTATAGATACGATTTAAAAAATATGCAAGATTTTGTTGAAGAAGTGTTTAGATTTTATGGATATGATAATTTCCCAGCTAAATCTCCAAAAATTACTAGATTATTAATTAAAGAAAACAAAGAATTTAAATTTATTGAAAACTTCTGTGCAAAAGGATATTTCAATGTTAGAACATACACGTTAATTAATCCTGAAAAAAACATTTTTAATCCATTTGATTTTAAAACTAATTTCAATGCTATTGCAGCAAAGAATTATGATCATTCTCAAATTAGAAATTCAATGATTTCTTCATTATTAGAAGTCTTGGATACAAATATCAAACAAGGTTTGCCAGCTGCTTCTTATTTTGAAATTGCTATGATTAATAATAAAATGAATGTATTAGGAATTGCTTCATTAAATAAAACTATTGATGATATAAAAGCTGATATTTGTTCAATAACTAATGAAACATTAGAATTTAAACCAAGTAAATTAAAAATTTTTAATCCAAATGCAGCACAAGAAATTTATTTAAATAACGAATTAATTGGATATGTTGCAAAATTAGACCCTCACTATTGTCAATATAATGCAGTGTTTAGCGAAGTCTTTTTAGATAAAATTAATAGCAACGTAATTAAATATAAAAATTATAAGCACACACCTCTTAAATCTAGGGATGTTACTATATCTGTTTTAGATAAACAATCTATTGAAGAAACAATTAAAAAAATACAAAGTATTTTTGGCGTGTACAAGATTTCAATTAAGGATGTTTATAATAAAGATAATGGCACAAAAAATATTACATTAGAAGTAATTTTAGAAGATTGAGCAACCAAGAAATTTGATTCAATTTTTAATAAATAAAATTAAAAAATGGTAAAAAAAGCCATTTTTTCTTTATTTTTTCTCTATTTTAAATTTTTAGGATATTTTATGAATATACTTAATTGCAGGAGTGAGAATGAAAGAAACAACAAATGATAATCTTCAAAAAGAAAAGAGCATTATTGCTGAAACATTAAAGGAAATAGAAAAGAAATTTGGCAAAGAAGCCGTTATGATTTTAGGAGAAAAGCCAAGCATAATTCCCGAAACTTTTAGTAGTGGTTCGTTGACTATTGATAGAGCGTTAGGAATAAATGGATGACCAAAAGGTAGAATTATTGAAATATATGGTCCAGAAAGTAGTGGAAAAACAACATTAGCGTTGCACGCTATTGCCGAAATACAAAAAACTGGTGGAATTGCAGCATTTATTGATGCTGAGCATTCTATAGATCCAATATATGCCAAAAATTTAGGGGTTGATATTGACAATTTAATTTTATCTCAACCAGATTCAGGAGAACAAGCATTAGAAATTGTTGATACATTGTCTAAATCTGGGGCAATAGGACTAATTGTTGTCGATTCGGTCGCTGCATTGGTTCCTGAGGCTGAGTTGAATGGAGAAATGAAAGACCAAGTAGTAGGTGCACAGGCAAGGCTTATGTCTAAAGCCTTAAGAAAAATAACTGGATCATTATCAAAAAACAAAACAACAGTAATATTTATTAATCAAATAAGAGAAAAAATTGGAGTTATTTTTGGCAATCCTGAAACAACAACTGGCGGAAGAGCATTGAAATTTTATTCAAGCATCCGTCTTGAAGTTAGAAAAATTCAAAATGTAACCAATAACGGAGACATTACAGGTAATCAAATTAAGTGTAAAGTTGTAAAGAACAAATTAGCTGCTCCGTATAAAACTGCTCAAATTGATATTATTTTTTCAAAAGGTATTTCAAAAGAGCTTGAAATAATTAATTTAGCAGAAGAATTAGGAATTTTAGTAAAAAAGGGCTCGTGATATAGTTTCAATGGTGAAAATATAGCACAAGGAGTGTTAAATTTACAATTTATTTTGCAAACTAATAAAGAATTATTCGATAAATTAAAACAAGAAGTTTTAGACAAATTAAATTCGATAAATTAAAATAGAAATTTAAACATATAAATGTTTATAATTTAAACTATGAAAAATGAAAATATAAAAATTCTATTTTTGGGAGACATATTTGGTTTACCAGGAATTAGATTTGTTGAGCAACAATTGCCAAACATAATAAAACAAAATAATATTGATTTTGTAATCGCTCAAGCAGAAAACGTTTCAGGAAGAAAGGGCTTTGAGCCTAAGGATTATGAAAGATTAAAAAGAGCAGGAATAAATGCATTTACATTAGGAAATCACGTTTGAGCTAAAGAAAAGATTCATCAAATAATAAACAACGGAGATTTAATAAGACCAGCAAATATAAATAGTTCATATCCTGGCAAAGGCATAAGAATATTTGATATTAAAGGCCATAAAGTCGCAATTATGTCTTTTATGGGCATAACATTTAATCCTCTATTAGAACCATGAAAAGAACAAAGTGCAAATAATTTCTTTGACGAATTTGATGAGCTATATGAAAATAATGGCGCAGATTATTACATAATAGATTTTCATGCTGAGACAACATCTGAAAAAGCAGTATTTTCACTATACGTTGATGGTAAAGTTGATGCAATTTTTGGAACGCATACGCACGTTCAAACAAACGATGCAAGAATTTTGCCTAAGGGTACATATTTTGTAACTGATGCCGGAATGTGTGGTCCAAGAGATTGCGCTATTGGTTCTGACTTCCAAGAAGTATATGAAAAAATGCGTTACGCAGCATTTTCTGCATTTAAAGTATCAAAAAATAAATGTCAATTAAATGGAGTTATTTTTACATTAACTAAAGATCCAACAGCAAAGCATATTGAGTTAGTAAATATAAAGGAGCAATAATATGAATAACGATTCATCATCAAAAATCAGTGAGAAGGATAAACGAATTTTATTTCTACTCAATAAATATAAGGCAAGGATGCATATTATTAATTGCATATATCAGAGCGAGCTATTTAATAAGAATCTTTCAATACAAGAAATTTTGGAAAATTGTGGAGAAAATCAATTGAATGCCAATGAAATAGAATCTTTAAAAATAATTGAAGCTAAATATGATGTTTTTACTAGAATAGCAAAAAAATTTATTAGTGAAGACTGAGAATGAAATAGAATTTTACCACTATCGAGAGCCATAATAATATACGGTGAATATGAATTATTATTTAATGACCCAAGGGTGGTAATTAATGAGATGGTTAATATTTCAAAAATATACAGTCCTAATAATGATTATAAATTTATCAATAAGGTTCTAGATCTTGTTTCTAAAAATGTAATTAAAAAAAATACTAAAGATGAAAAAGAAAATTAGCGAGATAATAACCAATATTTTTAAATACGATTTGAAAATTTCAGAAGCGTTAATTAGAGAAGGAAGAGTAATTGTAAATAATGAAAAGATAATTATTCCATCATTAAAATTTGAAGAAACCGATGTAGATATAAGATTCATTAATATGAAATTGCCCTATGTTTCAAGAGGCGCTTATAAATTATTAGAAGCAATAGAAAAATTTAATATAGACTTAACGGATAAAATATGTCTTGACATAGGATCGTCTACTGGGGGATTTGTTGATGTAATGTTAAGGCATGGTGCTAAAAAAGTATATGCCGTAGATTGTGGCACAAATCAACTAGCATATGAACTTAGGATAAATGATAAGGTTGTTGTTTATGAAAAAACTAATCTTAAAAATTTAAAAAAGGAAATGTTCCTTGAAAATATTGAATTTGTATCTTGTGATGTTTCATTTATAAGCTTGAAATATGTATTTGAGGTTTGCAATAAAATATTAAATAATAATATAAATGTAGTTTTTTTAATTAAACCCCAATTTGAAGCTTCAAGCAAATATGTTGAACCCGGAGGATATGTTAATCAAGAAAATCATAAATATATTATTGATAAAGTAATTTCATATGCAAAAAATAATGGCTTTGTTTTGATTGATTTAATAAAAAGCCCTATTTTAGGAGAAAAATCAAAAAATATTGAATATTTAGGCTTTTTTAGAAAGGATAGTTAATGAATTATAAGGATTTATTTCCTATGTTTAAAAATAACCCTGGTATTGTATATTTCGATAATTCAGCACTTACTTTTAAACCACAAACCGTAATAAATTCAGGTATTGATTTTTATGAAAAATATTCAATTTCTACAAGGACTGCTGATAGTGTTTTAGGAATACAAACAGAAGAAGTAATGCATGAAACTAGAAAAAAAATTGCAGATTTGATTGACGCTTCTGCCGATCAAACTATATTTACTTCAGGAACGACTGATGGATTAAACTTAATATCTTTAATGTTAAAAGATATAATAAAGGATGGCGAAATAATATTTAGCTATTTTAATCACAGCTCAGCAATCGTTCCATTCATTGAAAATCTAAAAAACAATAATATAACATTTAAATATGCAAAAGATAACGAGGAAGTTTTAGATTTAATAAATGAAAAAACGAAGTTAATTGTATTGCCTCAATTAACTAATAATTTTAATGTATTTTTTGATTTAAAAAAAATTCATCAAAAATCTAAAAAATATGGTGCATATTTGATAAACGATGCCGCACAAGCTATTGCTCACGAAAAAGTTTCATTGAACGACTGCGATGTAATTGTTTTTTCAAGCAATAAAATTTATGGTCCTACCGGTATGGGAGCTTTAGTAATTGAAAAAGATTTACTAAAAAAAATAGTTCCTCAAAAATGAGGCGGTGGCCAAGTTCAATCTATTGGTTCTGGTTGCGAATGAAGTGCCAAGAATGATATAAAAAAATTTGAACCTGGAACTCCTAATTTTGCTGGGATTTTTCAATTTAACGCTGCAATTGATTTTATAAATAGTCTAGGTTATGAATTAATAAATAAATTAGAAACAGCTATTGCAAATTATTGCTATGATGAATTAAAAACAATAGATGGAATAGAAATAGATTCAAAAAGAGGGGATAAAATTATTTTATTTAATATAAAAAATATTCCTTCTCAGGATATTGCATCATATTTAGGACATAATAATATTTATGTAAGATCTGGGGTTTTTTGTGCACATAAATTTCATGAATTGAAACAATATGGCAATAGTTATGTAAGAATTTCAATTTCATTTTATAATGATTATAATGATGTTGATAAATTCATTAAACTTTTAAAAGAAATGGGGGCTAATTTTCTTGAATTTTTATAGTAATATTGAAAAACAAAGAATAATTTTTGAAGCATACGAAAACCCAAAATTTAAAATAAAAAATGATGAAGATTTTAAGAAAACTGATAAGACATTATTTGAACATTCAAATGTATGCGTTGACGATATTAAGCTAAATTTTTTATTTGATGAAAAAAATACTTTGAAAAAAGTGGAATATTGTGCTAAAGGGTGTTCAATTTTTTTGTCATCTGTTGAATTAATGATTCAGCAAATTTTGAATAAAAATAAATTAGAAATAAATGAAATTTTAAATGAATATAAAACAATGATAGATACATCTACAATTTCACAACGTGGTGGAGAAATATTGGGAAAATTGGTTGTATATCAAAATGTAAGAGTTCATTTAAATAGGTTAGAATGTGCATCAATAATTTTTAGGTTAATAAAAAAAGCAGTTTCTAATGAATAAGATAATTTTTCACATAGATATGGACTCCTTCTTTGTTAGTTGTGAACGTTCTATTAATACAAACTTAATAGACAAGCCTGTTGTAATTGCTAAACGATACAAACGTTCAATAATTAGTGCAATGTCTTACGAGGTAAAAAATCTTGGATATAGAGTAGGAAGTTCTTTATACACCGTTTTAGAAAAGGTTCCTAATTTGCTTATAGTAGAACCACACTATGATTTATATTCTCTAATTTCTAAAAAAATTTTTGATTATCTAAAAAATAAGTATTGTACACAACTAGAAATATATTCAATAGACGAATGCTATATTGATTTTAGCAACACTGTTAGCAATTGGGAGGAAGCTAAAAATCTTGCCCAACAAATTAAAGATGATATTTTAAAAACATTTATGATTCCTTGTTCAATAGGAATTTCCTTTACAAAGTTTTTGGCAAAAATGAGTACAAATAAAGCAAAACCATCAGGAATTTTAATAACAAAAAAGGAAGATATTGAAAGAAATTTTTACTCATTGCCGGTGGAAAAAATATTTGGGGTAGGAAAAAATATTTCACAACAATTAATAAAAAACAAAATTTCTACTTATAAAGATTTGTTAGAATGCAATAATGAAATTTTTTTAAGAAAATTGTTTAAAAAAAATTTTTTCTTATTTATTGAACAACTAAAGGGAATCAATGCAACCGACCACATCTTAAATCTTGAAACAAAAGGAATAAGTAACACGATAACTTTTATGGAAAAAGATTCTGATGATATCGTTTTTTTGAAAAATGAATTGTTTAAATTGGCAAGAAATGTTGCTAATAGGGCCGTAAACAACAATTATGAATGCAGATGTGTTGAAGTTTTAATAAGAAAACAAGACAAAAAATGAATGTCAGAGCAAAAAAAAATTGATTTTTTTACAAATGATATTGACATTATTTTTAAAATTGCGTTGAAACTTTTTTTAAACTTATGAAATGAAAAAGAATGTTTAAGGGGTCTTGGAATTAGAATCTGCGATTTGAGGTCGATATTTCAAGAAAACAAACAAATCGATTTGTTTGAAGATAATAATCAAAAATCAATAGTAAATTCAATATTAAATGAGGTTAATGCAAAATTTGAGGGTAAAATTTTAATAACGGGGAAACAATATTTGTTAGAGAATTCAATCGATATTGATAATATAAAGTTTTTAAGAAAAAACAATCAACCATATGATAAAAAAATAAATTTAGAGGAGAAAAAATAATGAAGATAGGAATATTTGGCGGAAGTTTTGATCCTATTCATAAAGGACATATTTTAATTGCTAATGATGCAATTGAATTATTAAAACTAGATAAAGTTATTTTTGTTCCTGCTAACAAAAATCCTTTTAAAGATAAGCAAGATTATGCATCTAATGAACATAGAATAAATATGATTAACATTGTTATAAACAAGAGTAATATGAAAGTTTCACAATTTGAAACAAAAAGAGGTGGCACTTCATACACAATTGATACTGTAAAATATTTTGCTCAAAAGTATCCAAACGATGAGCTATATTTTTTAATTGGATCGGATAACGTTGGTAGTTTAAACAAATGAAAAGATATAGAAGAAATTTCAAGAATTGTAAAAATTGTTGTATTTAATAGAAATAATATTTATTCAAAAATCAATATTAAAAAATACAATTGTTTAGTTTTAAAAAATTCTTTTCATCCTTTTTCTTCAACTTCTTATAAAAACGGAAATATAAACGCAGTAGATGAAAGGGTTCAAGAATACATAGGAAAGAATTATTTATATTTAGAAAATATTGCCAAAAATACTCTTTCTATTCCTAGATTTAAACATTTAAGATATACTGCTGAATTTTCTGCACAATTGGCTAAAAGAAATAATTTTGATGTCGAATTAGCGTATAGAACTGGTTTTATGCACGATATTACTAAAGAGTGAGACACAAATAAAGCATATAAATTTTTAAGTTCATATGGGTATAATGAACAAAATTTGCCAGAATATATGCTACACCAAACCACGGCCTATTATTGGTTGAGAGATGTATATAAATATCAAAATGATGAAGCGTTACAAGCAATCAAAATTCATACATCATTAGATTTTAAAATATCTTTATTGGCAAAAATTTTATATATTGCTGACAAAATTTGTGAAGGAAGAAAATGACCGGGAATTCAAAAAATTAGAGAGCTTGCCTTAAATAATTTAGAAGAAGGTTTTAAGGAAGTAATTAGAATATGCGGTCTTAAATTTAATGAACAAAAAGGCATTATTTTTGATGAAAAGCAAAAAGAAATATATAAGTTATTATTAAAATAAAATGTGTTATAATGTATATGCTTTAACTAGCATAAAGTAGATTCATTTCTCACCTTAGTGATTTTTTGCTGGGTTGCTACAATAAAGAATTTGTAGTTTTTGGGAAGTGGATTAATACCACTTTTTTATTTATAAAAGAAGGAGGAGTTATTATCAATAACCAAAAAATTGGAGACGATTTAAATAACAATCAAACAAATCGTAAACCAAAGTCAGAACATGTTATCAACAATGCTATTCCATACAAAAAAGTTTTTGTTCTAGGACCTAGTGGGGAAAAAATTGGTGTGTTAACCAAAGAAGAAGCTTTAGAAAAAGCATCTGAATATAATATGGATTTAGTTTTAATTGCAATAGAAAATAATAAGCCAATAGTTAGAATATTAGATTATGGCAAATTTAAATATGATAAGAAAAAACGCCAAAAAGAAGTTAAAGAAAAACAAGCAGTTACTGTTAACAGAGAAATTCGCTTAACTCCATTGATTGGTGATCATGATTTGAAAACTAAGGCAAACAAAACTAGAGAATTTATTTTAGATGGTAATAGAGTTAAAGTTTCAGTTAAGTTTCGTGGTAGAGAAAGAGCAAGAATTGAATTAGGGGAAGAAATATTAAAAAAGTTCTTTGCCTTAATTGAAGATGTTGCAAAAATTTCAAAAGAAGCAACATTAGTTAATGATAGATTTTTAGATATGTATGTAGAAAAAGATAAACGAAAAGTTGATACATTAACCAATAAAAAAGATAACTAAACCTTCAATAGCATAAGGAGAATAAAATGCCAAAAATGAAAACCAAAAGCGGTTTGAAAAAAAGAATTTCAATCACTGCATCAGGAAAAGTAAAACGTGGACAAGCGTTTAGATCACATTTAGCACAAAACAAAACAACAAAACAAAAACGTCACGCTCGTAAAGCAACAACTTTATCAAGTTCAGATTTTAAAAGATATAAAGAATTAATTTAGAAAGGATAAATTATGCGTACTAGAGGCGGAATTGTAACAAGAAGAAGACGTGCAAAATGAATAAAATTAGCTAAAGGATATTGAGGACACAAATCAATAGGATACAAAGTAGCTAAACAAGCAGTTGTTAAATCATGGACATATGCATTTAGAGACAGAAAACAAGTTAAACGTGAATTCAGAAAATTATGAATTGCTCGTATTAACGCAGCTGCAAGACCACTAGGAATTACCTATTCAAGATTAATTGAAGGTCTTAAAGAAGCTAACGTGTTAGTAAATAGAAAAATGTTATCTGAATTAGCAATTAATTATCCTACATCATTTGCTAAATTAGTAGATCAAGCAAAATCAGCTTTAGCAAAAAAATAAATCTGTAATGGATTTATTTTTTATTATATTTATTAATAATTTATTAATAAATTAATTAAGTTCTTTATAATTTTATTTATATTGCGTAATGGAGGAAGATGAAAAAGAGAGTGGTATTAGGAATGAGTGGCGGAGTTGATTCTAGTGTTTGTGGATATTTGCTACAAAAACAAGGATATGACGTTGTTGGTTTGTTCATGAGAAATTGAGATTCATTTTTAAACAATGATATTTTAGGAAATGAAAATATAACTCAAAATATTTGCCCACAAGAGCAAGATTATTTAGATGCAAAGACGGTTGCAAGCCAATTAAATATTCAATTATATAGAATTGATTTTGTTAAGGAATATTGAGAAAATGTATTCAATTACCTAATTGATGAATATAAAAAAGGTAGAACTCCCAATCCTGATATTTTTTGTAATAAATTTATAAAATTTAAAGCTTTTTCTGATTATGCCTTTAAAGAATTAAAAGCCGATTATATTGCTATGGGGCATTATGCAAATGCAATTGACGGAGAATTATTTCGGGCGCAAGATGATAATAAAGACCAATCTTACTTTTTAGCGCAATTAAGTCCTGACCAACTTAAAAAAGTAATTTTTCCATTGGCTAATATTACCAAACCAGAAGTACGAGAAATTGCAAAACAATTAAACTTGTCAACCGCTATGAAAAAAGATTCAACAGGAATTTGCTTTATAGGTGAAAGACACTTCACAAAATTTTTAGAAAATTATATTCCTGCTCAACCAGGCAATATTATTGATATATTTAGCGGGAAAGTGGTTGGAAAACATATTGGTGCGATGTATTACACTATAGGCCAAAGAAAAGGTTTAAATCTTGGCGGGTATGAAAAACCATATTTTGTAGTAGGACATAATCTTTCAAAAAAAGAAATTTATGTAACAAACGATGATCATATTGACTATTTAATGTCAAATAAATTAATTGCAACAAATTTTAATCAAATTTCAAAAGAATTTAATTTTGCTAATTTAACTGCTAAGTTTCGTTATAGACAAAAGGATATCAAAATAACTTTAAAAATTTTAGATGATAATAAAATAGAAGTTTGCTATCCAAATACCGAAGCAGTAACTCCTGGCCAACAAGTTGTAATATATGATGGAAATAAAGTAGTTGGTGGAGCGATAATTGACAAAATTTATTTAAATAATGAATTAAAAACGTTTTTATAAAGGAAAGGAAAATAGAATGCTAACTTCAAAAGAAATTAGAAAAATGTGATTAGATTTCTTTAAATCTAAAGGACATTTAGTGGTACCTTCAAAATCTTTGATACCACAAAATGACCCCTCTTTGTTATGAATTAACTCAGGAGTTGCAACATTAAAAGATTATTTCAGTGGTAAAAAAATACCTCCTGCTAAAAGAATTACCAATTCTCAAAAATCAATTAGAACAAATGATATTGAAAATGTTGGAATAACAGCAAGACACCATACATTTTTTGAAATGCTTGGTAACTTTTCAATAGGCGATTATTTTAAAAAAGAAGCAATTGCTTTTGGATATGAATTTATTTTTAATGTTCTAAAACTAAATAAACAAAATATTTATATAACTTATTATTCAGAAGATAAAGACACTTATAATTATTGAATTTCAAATGGTATTGATCCTTCACATATGATAAAAGGCAGTAGAGATACAAACTTCTGAGATGTTGGACAAGGACCATGTGGCCCTGATACCGAAATTTTTTACGACCGTGGGACAAAGTATGATACCCGTGGCATTGAATTGCTAGAAAACGATATTGAAAATGATAGATATATTGAAATTTGAAATATCGTATTTTCACAATTTAATAACGATGGTGAAAACAATTACACCGAATTAGCAACAAAGAATATTGATACTGGTGCAGGACTTGAAAGAATAACATCAATCTTGCAAGATGCTCCAACTAATTTTGATACTGACTTATTTTTACCAATAATTCATGCAACAGAAAAATTAACAAACAAACATTATGATATTAATAACTATTTTACAAAAGAACCAATTCAAATGAAAATTAACACCCAATTTAAAATAATTGCTGATCACATTAGAGCTTCAGTTAATGCAATAAGTGACGGTGTTAAACCTTCAAATATTGGTCGTGGTTATATTATTAGACGTCTAATAAGAAGAGCATATAGGGCAGGTATTTCTCTTGGCATAACTCAAAAAGCATTTTTATATGAACTTGTTCAAATAGTTAAAGATTCATTAATTTATGACATTGATGTTGAAGAAGTTAAAAAAGTTATTTTAGATGAAGAAAATCTTTTTGCTAAGACTATTAATGAAGGTGAAAAACTATTAAATCAAGAAATTGAAAAAAATGGTACCATCGATGTTAAAATAGCCTTTAAAATGTTTGACACATATGGCTTTCCTATTGAACTAACAAGAGAAATTCTACAAGAAAAAAACATTGACATCGATATGAATGAATTTAACGAATTTTTAAAGCAACACCAAGAAAAATCAAGAGGAAATATTGAAGCCGGAATGCAAAAAGTTATTAATTCACTTTCATTAATAACTGATAAGATATCTGAATTTGTGGGATATGATCACACTAAAACAACTTCAAAAATTTTATATTTATTAAATAGCGAAGAAAAAATAGATAATGCGGGCGAAGACGAAGTTTCTTATTTAATATTAGATAAAACTCCTTTCTATGCTACAAGTGGTGGCCAAAATCATGATGAAGGTTACATTTTACAAGGAAATAATAGAATTGAAGTTTTAGATGTATTTAAAGACAAATATTGAAATCATGTTCATGTTGTAAAAGGCAAAATAAATTCAAATGAACCTGTTCAATGTTTTGTTGATGAAGATAAACGTATTAATTTTGCAAGAAATCACTCTTCAACTCACTTGATGTTTAGTGCATTAAGAGAAACATATCCAAACGAAATTATCAAGCAATTAGGTTCGAATATTACATATGAACATTTTACATTTGATTTCGGTCTTGATCATAGGCCAACTAAAGAAGAGATAAAATTAATTGAACATAAAATGAGGCAATATATTGCTAGAGATGTAAAACGTAATTACATAATAACTTCGCTTAAAAAAGCAGAAGAAATGGGCGCATACATGACAATTGATGAATCAGAATATCATGATGCAAATTATATAAGAGTTGTTGAATTTGAAGGTATTACCAAAGATTTATGTGGTGGAACACACGTTCCTCATTCAGGTGTAATTGAAGATTTTAAAATTGTTGCCGTTGATTCAAAAGGTACAGGAATATATAGACTTAGAGTAGTTACTTCATATGATTTAGTAAGAAAATATTTATTATCAGAAATTCAAGAAAAACAAAAAATATTGGATACATTAATTAGAAATAATCAAAAACTAAATGAAGGCTATAACTTTAAATATACAAAGGAAATTATTTCTAATAAGACATCTGATTTAGAAGAATTACTTGAAGAATATGATAAATGCGAAGAACAACTAAGAAATGATTATCGAACATTATTGAAACAAAAACAATCAGAAGATGTTGATTTAAGTTTGTATAATTATGAAACCATCAACGATAAAAAAGTATTCTTATTAATTAACATAGAACAATCATTAATTAAAAAAGTTTCAATCTCATTAAGAGAAGCAAATCCTGATTCAATTATTATTATCTTAAACAAGAGCAAACAAGATTCATATTATTTTGCAGTAGCATCTAAAAAATATGATTCAAATGCAATATTTAAAAACTTGGGCAAGGTTTTAAATATTCATGGCGGTGGAAATGCAATAATTTCTCAAGGTTCATTGGTTTCAAACTTGAAACAAGAAGAAATTATTGAAATTATTAAGAGCAATTTATGCGAATTTTAGCATTGGATTTAGGCACAAAAAGTTGTGGCTTTGCAATAAGTGATCCTAATAATATAATTGCAAGTGGACTTGAAAATTTTAGATTTGAATTGAATCATTTTAACTTAGTTATTGAAAAAGTGCTACATTATTTAAATAAAAGTGAATATCAAGGTTCAATTGGCACAATAGTTTTAGGATATCCCTTAAGAATGGATTTATCAAAAAGTCAACGAACATATATGGTTGAAAGATTTGGAGAAAGATTAAAAAAAGTTGTTGATATTCCAGTTGTATTTCAAGATGAAAGACAAAGCACTATTGGAGCTCAAGAAATATTGCAGAGCGCTGGATGAAACACTAAAAAATCTAAATCAAAGAAAGATTCACTAGCAGCGCAACTTATATTAGAAGATTATTTAAAAGGAATTAAATATGAATAAAAATATTATTAATTTAGATGTAGTTGATAGGCAATTGACTACAAGCGATGGCGAAAAACTTTATGTTATTTTTGATATTGAAGAAAACGGCGAACATTATCTTGTTTTAACAGATTATGACGCGATTATTTTTGCTAAAGAACAAGATCAAAATTTAATCGAAGTAACAGATGAAGGTGAAATTGACATTCTTGTTGATTTGACAATGGAATTTGCAGAAAATAATTTTGTTTTAGATAAAGACGGAAAATCAGATTTAATGAAGAAATTAATTGGCAACGATCAGGGTGAAAATGAAGCATAAGGGTTCAATATTTAGCGCTAATACAAAATTAGGTTTAATAATCTCTGTCGTCGTTGTATTTATTTTAATGTGTGTAATTTTGTTAATAACTATTTTAGCACGTAGACATTTCTTAAAAAGCACAATAACATCAAGCGAAGAACGAGAACTACAAAATTTAAAAACAAAAAATCCAAATTATGGAATTGTTTTGAGTGGCATTAAAAAATATTATGATAATGTTTTAGGCGACCATTTGATTGCGTTTTTAATTAATACAATATATTTGAATAATTTTCTAAACGTTAGTCTTATTGAAAAAAATGATTATGCTTCAATTTCAATAGCAGCATTATCTATGGCTAATGTTTATTCAAAAAATCAATATTTAATTCAAAAGCAAGGTTCAAATATTCAAAACGAAAATCCAGATTTAGATCTATCTAGATTAAATTATGTTGATGAAATTTATAATCCTTCGGATATGATAATATCTTTATGCGATGATCTTTCAATAGAACAACAAATTAATTCAAATTTACCTTATTTAAGTGATAGAGGTATGCTAATTGTTTCATTTAAGACTATAAAAGACTTTAAAAATCAAAAAAATGTTATTTTAGATAATAATTTAAGATACGAAACTGTTAAAATTAGTAATAAAAATGTTATACTTTTAGCAAAAAACAATATGAATTCAAACAATGAAAATTCTAAGGAGCAACAATAATGGCTAATAATGATAATAAGATTTATTTAACAGAAGAAAGTTTAAAGCAATATCAAGAAAAATTAAAATATTTACAAGAAGTTGCAAGACCTCAAGTTATTGAAGAAATTAAAGAAGCAAGAAATCAAGGTGACTTGTCAGAAAATGCTGAATATGACTCAGCTCGTGATAAACAAGCTGCTATTGAAAATGAAATTTTAGAAATTCAACATATTCTTGAAAATGTTGAAATTATTAAATCAACATCAACTCATACAATTAGAATTGGTTCAACTGTAAAATTAATAAATATAAACACAAACGAAATAAGCGAAGTTAAAATTGTTGGTGCATTAGATGCCGATCCATTCAACAATAAAATTTCAAATACCTCACCGCTTGCAATTGCATTAAATGGCAAAAAAGTCGGACAAGTGATTGAAGTTGAAGCTCCTAAAAAATATAAAGTTAAAATTGAATCAATTTTATAATTTAAAAATATTTGCACTGTGCAAATATTTTTTATTGTTATTTTATAAAATAAAATATTTTATTTAATGAAACAATTTCACACATTAATTTATAATATCTAATAATGAAAAATGAAGTTGTTAAAAATAATTTAGGATATAGCGGAGATTTATTTGTATATCAAGATAAATCAATGTTTAATTATTCTGTTGATACTATTTTACTCGGCAATTTTGTATCAATAAACAGAAAAGTAAGAAATATTTTGGAAGTAGGCACGAATAATGGTGCCCTTTCTATTTTTATTGCTGCAAGAGATAAACGTTTGAACATTGATGCTATTGAAATCCAAAGCAAAGCTGTTGAGCTTGCAAGAAAAAATATTATTTTAAACAAACTTGAAAATCAAATTAATCTTATTGAAGGCGATTTTCGGATGTGGGCTAATCAAAAGGCATACGACTGTGGTAATAAATTAGCCAAAAAATACGATTTAATTGTTGCTAATCCGCCTTACTACAATGAAGATTATAATCAACAAAGAAAAAACTCAACTCTTGAACAAAAACTAGCAACTCATGAAATAAATTTAAATTTAGAACAATTAGTTGCTAATAGTGCAAGAATTATTGAACAAAAAGGATACTTAGCAATTGTTTTGCCAATGGCAAGATATATTGATCTAATTTGTTTGCTAAGAAAATACAATTTTGAACCAAAAAGAATTCAATTAGTTTATCCAAGAATCACTGATTTACCAAAATTCTGTTTAGTTGAAGCCAGGTTTAATTCAGGTTGAGGAACATATTTTGAAAGTAATATTTATTTACATAATGAAGATAAAAATGATCACAGTTATAGCCAAGAAGTAAAAGAATTATATAAACCGAAAAAATCAAATTTTTAAATATAAACATTGAAAGGAAAATATGAATAATAATAAAAAGACATTTTATATTACCACACCAATTTATTATCCAAGTGGTAATCTTCACATAGGACATTTATTAACTACTACATTAGCTTCAGTATATAGAAATTACAAAAAACAACAAGGATATGAAACCTTGTTTATTACCGGAATTGATGAACATGGTCAAAAGATTCAAAAGAAAGCCCTTGAACAAAATATTACTCCAAAAGAATATGTAGATATTCAATCTCAAAAGTTCGTTGATTTATGAAAATTATTAAAAATTGATTATGATAAATATATTCGTACAACTGACGATTATCATATTGAAACAATTCAAAAAGTTTTTTATTTAATGGAACAAAAAGGCTATATTTACAAATCTAAATATGAAGGCTTATATTCAATTAGCGATGAAGAATTCTTAACTAAAACTCAAGCAATATCGAAGGATGGAAAATTTTACCACCCAATTTCAGGACACGAACTTCAAGAAGTAAAAGAAGAAAGTTATTTCTTTAATATGTCAAAATTTACCCCTTGAATAAAGTCATATTTCGAACAAAATCCTGATTTCATTACCAATAAAGCAACATATAAAGAATTAATGAATAATTTTATTAATAAAGGATTAGAGGATCTTTCGATAACAAGAGTTTCTTTTGACTGGGGAATTAAAATAGAAACTAAAGATGCTGAAAAGCAACACATTATTTATGTTTGATTAGATGCTTTATTTAACTATTTATCAGCATTGGGATACAACAATTACCTAGGCCAAGAGTTATATAATAAATTTTGGGTAAATGGTAATGAAAGAGTTCATTTATTAGCTAAAGAAATTTCTAGATTTCATGCGATATATTGACCTATATTTTTAAAATCTTTGGATCTTAATTTACCAACAAAATTAGTAATTCACTCATGAATTGTTACCCCTGAAGGAAAAATGTCAAAATCAAAAGGCAATGTTATTGAACCAATTCCTTTAATTGAAAAATATGGGGCAGAACCCGTTAAGTATTTTTTTGCATCGCAAATAAATATTGACAATGATTTTAGTTTTAGCGAAGAATTACTTATTAATGTATTAAATGCTGATTTAGCAAATAATTTTGGTAATTTGGCCAATAGAACCTTTAAAATGGTAATGTTAAATTTTGAAAATGGTACAACTTATTGTTCAAACCAATTGAATTCATTAGATAAAAATGTTTATTCAGAAATAGAAAATGCATACAATGATTATAAAAAATATTTTGACAATTTTAATGCTGATAAGGCTTTAAAAAGGGCAATTGAATTAAGTAAGTTTTTAAATGAATATATTGATAAAAATGAACCTTGAAAATTAAAAGAAAATTTGCCAAGATTAAATGTGGTTTTAAATACATTGTTGAATGGAATTTATGCAGTTAATATGATGTTAAGCGTTGTTTTAAAAGAAAAATGTAACATTTTATTAAATGATTTGCAATTGTCTGAATTCTCTGAAAAAATGTTGTTTAATTATTCTAAATTTGATAACAAAAAGTTTTCTAAACCTAATATTTTATTTGAAAGGATAAAACCAAATAATTAAATTATTGGTAAAATTAGTTCATGAAATTTATAGATGAAACAAATGTAATAGTAATAGCTGGCAAAGGTGGCGATGGCATAATTAGTTTCAGAAGAGAAGCTAATGTTGACAAAGGCGGTCCTGATGGTGGCGATGGTGGCGATGGTGGCGATGTTTATTTTGTAGGTGATCCTGGCCAAAATACACTTCTACCATTTTATTATCAAAATAAATTGGTCGCTGAATCAGGAGAAGATGGAAGGCCAAAAAATCAATATGGTGCTGATGGCCAAGATTTAATTGTAAAAGTTCCACTTGGAACAATGGTTTATAATGGCGATGATTTAATAGCCGATGTAGTTAGCCAAGAGAAATACTTAATAGCAAAAGGTGGACACGGTGGCAAAGGAAATCCTAGATTTAAATCTAGTACTAACACTGCTCCTAGAATATGTGAAAATGGTACACCTGGTCAAAAATTTGAACTTCACTTAGTTTTAAAAGTTTTAGCAGATGTTGGCTTTGTTGGAAAGCCTAGTGCTGGCAAGAGCACTATTTTAGGCGAAATATCTAATGCAAAGCCTAAAATTGCAGATTATGATTTTACTACATTGGTTCCTCAACTAGGTTTGGTAAAATATTTTGACAACTCATACGTTGTAGCAGATCTACCAGGACTTATTGAAAATGCTCATTTGGGCAAGGGTCTTGGAATTCAATTTTTAAAGCATATTGAAAGATGTAAAATTATTGCTCATGTTATTGATTTTGGTAGTGAAGAAAAGAATCCAATAGATGATTATGAAATAATTAATAATGAATTAAAATCATATAATTTGTTGCTTGAAAAATTACCACAAGTAGTAATAGCAAACAAAAAAGATCTACCGGCTTTTTCACTAAACTTATCAAAATTTAAAGAAAAATATCCAAATATAAATGTATTTGAATATTCAGCATTAATGGCTGAAAATTTAGATGAATTAAAAGGATGCTTGTTTGAAACATTGCAATTTGCTAAAGATTCAATTGCTGTTCAACAAATTCATGAAAGTCAAAACATTGTAAAAATAACATTAAATAAAAAACCTATTCAAATTAAAAAAATTGATAATGAAACATATGAATTGTTTGGTGATGATATAGATAATATATATAATAAGATACCAGTGATTTCATTAGATAATTTATGAAGATTTAATGCCAAATTAAAAGCATTAGGTGTATTTGAATTAATAAAACAAAATAATATTAAAGACGGTTCCACAATAAAAATTCATGATTATGAATTTATTTGGAATGCTGAAGAATTTTAAAATTTGTCAAGATTTTATTATTTTTTAAAAATTGCATATTACTATGTAATAAAAAAAATTTTAATTTTAAAACTTTTAAAAAAATAATATATAATAATTAAGCATTAAGCAAAAGCAATAAGTATTTATTTATAAACAACAATTGTGGGGAAGTAGCTCAGCTTGGTAGAGCACTTGACTTGGGCTCAAGCGGTCGCAGGTTCGAATCCTGTCTTCCTCACCATTTATGGGGGGGTAGCTCACCTGGCCAGAGCGCCTGCCTTGCACGCAGGAGGTAGAGGGTTCGAGTCCCTTCCTCTCCACCATTATGGCACTGTAGCTCAGTCGGTTAGAGCGTCCGGTTCATACCCGGAAGGTCAAGAGTTCGATTCTCTTCGGTGCTACCAAATGCAACAAGCCTAACGGGCTACGGACCCATAGCTCAATGGTTAGAGCAACCGGCTCATAACCGGTCGGTTACAGGTTCGAGTCCTGTTGGGTCCACCATTGGGCAATTACCCAAGAGGCTGAAGGGAGCAGTCTTGAAAACTGCCAGGGGCTTTACGGCCCGCGGGGGTTCAAATCCCTCATTGCCCGCCATTATATAGCGGAGTAGAGCAGGCGGCAGCTCGTTGGGCTCATAACCCAAAGGTCATTGGTTCAAATCCAATCTCCGCAACCATGGTCCAGTGGTAAAGTGGTTTAATACGCTTCCCTGTCACGGAAGAGATCGCGAGTTCAATTCTCGTCTGGACCGCCATGGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCACTGGTTCGATTCCTGTCGGAGCCACCATTTCAGAGATGACTAACACCTTAGGGTGTTTTTTTATTTTTTATGTTTAATAAAAGATAAAAAAAGTAAAGTATAATAATACATGTTATGAATATTAATAGTATAAAAATTGCAATTCAATTATTTGGAATTTTTGGTTCAATTGTAACGATTTGTTTAGGAATTCCACAATTAATTAGATTAAAAAAAACAAAAAACACGGGCAAAGTTAGTTTTGCAGCATTTTGAGTTTTTTATTATGGAATTTTAGCGTGACTAGTTTTAGGAGTATTTACTTCTAGCGAAAATTCATGATATATATTCCTATCTAACTTTGCATGTGTTACAATAACATCCTTTACAGTATTTTACTTGTATTGATATTATGAAAAATTAACTAAAAAAATGTTAATCAGGGCTATTGTTGGAATAAGCATTAATCAAGTTATTTCATTAATTTTCTTGTGCTTATTTTTTGTTACGGTATATCAAAAGTTAAAATTTGGTAAATTTTATCCCGATAATTCAATTATTCCTGTTCTTCCGGGTAATGTACAACTTGCATTTGGACTAATATGTCCCGCCTTTACAAGTGCAGCATTCTTCCCTGGATTAATTGTTAGTTTAATTAAAAAAGAAGAAATAAGTTTAAGCCCATGAATGGCTCTTGTTTATATGGTAAATAATACTTTTTGAATATTGTATTTCTCATTAAATATTGTTTATTCGAAGTTTGGCGTGGATCAAGCAGCACTTAATAGTATGCCAGGATTTATTGGGGGACTAGTTTGACAATTACTAGCATTTGGAATGTTTGCTTTCCAATTTGGAGTAACATTACACTTTGATATTAAGCACAAAAAACAACAAAAGCAAGTAATGGTTAACGTTATTAATCAAAATTAATCAAATTAAAAGTAGCAAATTATTGATTGCTACTTTTAATTTCATCATTTGCAACTTTAGAATATGTTCTAATTAATTTACTTGCGCCTAATTTTATGCCGCCAAAATATCTAACTACAAACAACACACAATTATTTAATTTATTTCTTTCTAGTAAAAAATATAAAGGTTTGCCTGCTGTTCCTGATGGTTCACCATCATCACTCATTCCGCCACATTGAACGCCATTTTCAATAACTAAATAAGCATAAACTATATGTCTTGCTTTTTGATGTTGATTTTTTAATTGCTCTTTTAAAAGTTTCACATCATCTTTACTTTTAATATTAAATTTATAAGAAATAAATTTAGACTTTTTAATTTCTAAAATTGCCATAGTTTTATTATAATAAAAATTATTGTATATAATTACTAACATGTTAAAAATTATTTCAAATTATTCAGACAGAATAGATAAATATGTTGCTAATAATAGCGAGATTTCAAGAAACGATATTAAGGAATTAATTGAAAATGGCGCAGTATTTGTAAATTCAATAAAGATAAATAAACCTAAATTCGTTGTTAAAGAAGGCGATGAAATTTTAATTGAAAGATTATTAGATAAACAAATAAAAGTCGAACCTCAAAACATTCATTTAGATATAGTGTATGAATGCGATGATTATTTAATAATTAATAAGCCATCAGGCTTAGTTGTTCATCCGGCTCCTGGACATCATGATAATACATTAGTCAATGCTTTGATGTATCATTTTAAAAATAATTTAAGTGATGTTAATGGTTTATTACGCATGGGGGTTGTTCACAGAATCGATAAAGATACTAGCGGTTTATTATTAATTGCTAAAAACAATAAAGCGCATAACTACTTTGCTTCTCTTTTAAAAACACATGAAATTAAAAGGAGCTATTATGCTATTGTTGAAGGCAAGATTGCAAATGAAGAATTACATATTGAACTTCCAATAGGTAGAGATACTAAAAATCGCCAAAAATATGCTGTTAGTGAAACAAATTCAAAACCTGCTTATACAATAGTAAATGTTGTCAAATATTTTAAAATGAATAATAAAGATTTTTCATTGGTTAAATGTAATTTAAAAACTGGTCGTACTCATCAAATTAGAGTTCACTTAGCATATATTAAACATCCTGTTTATGGAGATCCAATTTACGGTCATAAGATTGATGATTTCAATCAAAGGCTTCATGCATTTGCTTTAGATTTTGTTGATTTAAATGGAAACAATAAACATTTTGAAGCAGAAATGCCTGAAATTATGAAAAAAGAAATAACTGGCGAATAAAAAAACCTAAATTAAATTGCAATTTAAAATATAATTATTAAATATAAAAGGAGTTTTAATGAAATCAATAAACATAAATGAATATAATTCTACAAATGAATTATATACAAAGAACAAAAGAGTTTTTTGGGCTTGATTCATTGCTTGAAGCGTTTTATTTGCAACAATTTTAATATTTGTAATTGCTTCTTTTATTCAATTTGCAGTTGAAAAACAAAACTATTTGAATAAATATGCTGAACTTTTTGGCCAAATCCAAAAATTAAATCCAAATTTTCAAGGAGATGTATTTGCACTAGCCAATAATGAATATGTCAAATCTTTGGCAATGTCAGGCGTTTTACTTGTAATTTTAATTGCGGTTTATGTTTGACATATTGTTTCCATAGCATTGGTTGCCAAAAACAAAGACTTTTCTGCTTATTCAAGGATTTTAACAACATTATATTTATTTGTAATTATTTATGAAATAATAACATTAATTTTTTCTGGATTTCAATCATTTTCATTGGCAATTTGACAAGTAAATAAAATTTTAAATTTAGTGGCAACATTGTTATATATAGTTTCATATTTTGCTGTATTTTTAAAATGCTCTTATGTTGCAAAACTATTTTTAATTTTAAAAAGAAATTTAGCATTTAAGGCATTAAACAACTCGGGCTCGATGGATGATATTTTAAGATCATATTTCTATGGTCAAGAATCTCCTGAATCAACACTAAATACCAATAATACATTTGATTCAAATCAAAAAACCGAAGAAAACAAATCTGAAGATTTAAACAAAGAAGAAGATTCAAAATCAATTGAATATCGTAGTAAATTAGAACAATTAAATGATGAACAATTGCAAAATATGGCCAAGAAATTAAATATTTTTGGAATTGAAACATTTTCAAGAGAACAATTGATTTCAAAAATTAGCGATATTTTTGAACAATCAGCCAAGTCTCAAGATCAACAATCAAAACCAAATAATCTTGACGAAGACAACAACGGTGAAGGCCAAACCAACAATTAATTTTTTGTAATTTTATAATTACTATTTAACAAGGAAAAAACAATGAATAATCAAACATCATTAAAACAACATATTTTTACTTTTTTTTCACAAAATTCTCCTGCTAGTCCAATAAAAAATGGTGATCAAAAATTATGAATTCCTGCATTAGTTTGCATTATACTAATTTTATTTTCTCTATATCTAGCAATAAAATTTTTTGTTAATTTCAAACGTAGCATTGCTAAAACTAAATCTATTTTAAAAGACGATCTTGTTAGTGCTTATGTACAAGGCTTTAATATTAAATCCTCAGCAGTTTTTAATTTTATATTAGCTAACATAATTTCTTTAGTTTCAATTATCATTTCTATAATATCAATAGTTAAACATTTCTCAGCAAGCTCAACGTTAGGTAAGTCTTCGTTTCTAGCGTTGTTTATAGCAGTGATTGCACTTTCAATTGTTTTAATAATATCAATTAATTTATCACTATTAGCAATTTATTTTGTTTCATTTAAAGAAGCTAAGTTTGTTAATAAAAAAAATTTAGAAGATGAGCTTATTTCTTTGAAGTATAAAAAATCCAAAGAATTAAATGATTTAAATGCTCCTGAAAATATTAAACTAGATATCAAAGCAATGGAATCACACAATCCATTAGCACCTAGAGTGTTAGCAAAATTTATTGATTTTTATAACAAAATGTCAAATATCGAATTATTTAAACGTTATAAAAATTATTTAAATCAAACATTCAAAATTAGATATTTTAATGAATCCCTTGAAGTAATTGAGGGAAAAAATGAAATGGAAGATAATCTTGAAAAATATTTAGACAACATAAATAATCAAGATCCTGAATTGCAAAAAAATTCTCTAATTTTAAAGGAAATTGCTTGAATGAATGATATGGATAAAAAGGTAAAAATTATTAGAGAAGCAGATAAAAATATAACCATGTCTAAAAAAGAATTTCAAGAAAAATATGATGAATATGTTTATTTGCAAAGATCACAAGATCCTGCATATCAACAAACTCAAAAGAATACATGACTTACATATCGTGATTCCGATATTGAAGATCTATTTTATAACACAAATACATCAGTTATTTATACCATTGATAATGGCAAAACAATTTTAGAAAAACCAATGAATGAATTTTTAAATGAATATGGACAATATTTAGAAACCAAATTTTTTACATCAATTAAAGAAAACGAAGAACTTAAATAAGAGATTGTAATGACTAAGAAACACAAAAGAATTTTATTTTTATCAACATTAGCATTACCCGTAGCCCTTGTACCTGCAACTATGGTTTCGTGTTCAAAAAAAGATATTGAAGAAATAAAATTGGATATGATCACAATAGCAAAAAATCAAATGCTAAATTATTTACAAAAATTTGAATCATTTTTTAAAAATGATGATGATCCTGATGTTAAAAATTTAGAAATAGAATTTAATAAAGTTATAATTTCATTAAAAAAAATGGATATAACACCTGATTTTAATATGGACCAATTTATTCAAGAAATGACTAAAAAATTCAGTGATGTAGCAAATAATTTTTTGAATTTAAAATACAATAAAGCCAAATTGACAAGTGAATATTATATTTCAAAAGTAAAAGCTCAGCTAATTTTGGAGCAATTTAAAAATCCCGGTTTGTTTCCAAAAAAGAACGAATTTGCCAAGATTTTAGAGCAAGAATTATCAAAAGTTTCCGATCCAACTATTAAAACTTCTGAAGAAGAGTTAAAAAATTCAATTAGATTTTTAGATGATTTAAACAAAAAATATGATGAAATTTTAAAAAAGCAAGTAATGATTTTAAAATAAAATTTCTTGCTTTTTTTATTTTTTGTTTTTATTTAAAAAAGTTTAATTTTTTTTAAAAACACCGGGTTAGTTGTTGTATAATTGTACAGTATTTATCTATTAAAATACCAAAGACCATATAAAAATACAATATATAATTTAAATTTTAGAAAGGAAAAAATATGCCTACAGTTGCACAATTAGTAAAACAAGGCCGTAAAGATAAAACAACAAAATCTAAAGCTCCTGCTTTAGGAAAAATGTATAACTCTTTACAAAAAAAAGAAACTGCTATGCCCGCACCATTTAAGCGTGGCGTATGTACTCGTGTGGCAACAATGACACCTAAGAAACCTAACTCAGCTATTAGAAAATATGCCCGTGTTAGATTATCAAATGGTCAAGAAGTTACTGCCTATATTCCCGGTGAAGGACACAATTTACAAGAACACAGTGTTGTTTTAATTCGTGGAGGTAAGGTTAAAGATTTACCCGGAGTTAGATACACAATTGTTCGTGGAACACAAGATGCTGCCGGAGTTAATAACAGAAAGCAAGCTCGTTCTATTTATGGAACTAAAAAACCAAAAAGTAATTAATTATAGTTAGAAATTAGAAAGGAAAATCATATGTCAAGAAAACATAAAGCGCCTGTTAGAGATGTCTTAGCAGATCCAGTTTTTAACTCTAAGATAATTACTAAATTAGTAAATACCATTATGTTAGATGGTAAAAAATCTATTGCTGAAAACATTTTATACAGTGCATTTGATATTATTAAAACAAAAACAGGAAAAGAACCAGTTGAAGTATTTAATCTAGCATTAGAAAATGTTAGTCCACAACTAGAAGTTAGATCAAGAAGAGTTGGTGGTTCAAACTACCAAGTTCCTTGTGAAGTTTCTGCAAAAAGAAAACAAACTTTAGCATTAAGATGAATTATTCAATATGCAAGATTAAGAAACGATAAAACTATGGAAGAAAAGTTAGCAAATGAATTAATAGATGCCTCAAACAAAGTTGGTGGCGCTATTAAGAAACGTGAAGATACCCATAAAATGGCTGAATCTAACAAAGCATTTGCTCACTTTAGATGATAATAGGTTGGTTGTATGTCTCGTGAATATAAATTAGAAGATTACCGCAATATCGGAATCATGGCTCACATTGATGCCGGAAAAACCACAACTACTGAACGTGTGTTATATCACACAGGAAAAATTCATAAAATTGGTGAAACACACGAAGGTGCTTCACAAATGGACTGAATGGTTCAAGAACAAGAAAGAGGTATAACTATTACCTCAGCTGCTACTACAGCTTATTGAAAAGGAAAAAGATTAAACATCATCGATACCCCAGGACACGTTGATTTTACTATCGAAGTTGAACGTTCGTTAAGAGTATTGGATGGTGCAGTTGCTGTTTTGGACGCCCAAAGTGGTGTTGAACCTCAAACAGAAACAGTTTGAAGACAAGCAACAAATTATCATGTTCCAAGAATAGTTTATGTTAATAAAATGGACAAGATGGGTGCTAACTTTAAAGCATCTGTTGAATCACTTCATAAATTACTTGGTGCTAATGCTCACGCTATTCAATTAAATATTGGTGAAGAAAACCATTTTGTTGGAATCATTGATTTAGTTGAAATGAAAGCATATTCATATGATGGCGAAGTTGATGAAAACGCTAAAGAAATTGCTATTCCAGAACATTTAAAAGATGAAGCTCATATTATGAGGGCTGAATTAGCTGAATCACTTGTTGATTATGATGAAGAACTTATGACTTTATTATTAGAAGAAAAAGAAGTTCCAGTTGACATTTTAAAAAGAGCAATTAGAACTGCTACAATTTCTTCTGAATATTTCCCAGTTGTTTGTGGAACATCATTTAAAAACAAGGGTGTTAAGTTAATGCTTGATGCAATTGTTGATTATTTACCAAGTCCTTTAGATATTCCTCCAATGAAGGCATATAAAGGCGAATCAGAAATTCATATCCCTGCCTCAGATGATGAATTTTTCTCAGCTTTAGCATTCAAAGTTATGAATGACCCATTTGTTGGAAACCTAACATACTTTAGAGTTTACTCAGGAATTGTTTCAAAAGGTTCTTATGTATTCAATTCAACAAAAGGTGAAAAAGAAAGACTAAGTAGAATTCTATTGATGCATGCTAATAGTCGTACAGACATTGACGAAGTTAGAACAGGAGATATTGCTGCAGCAGTTGGTTTAAAAGTAACAACTACTGGAGATACATTGATTGATGAAAAACAAAAAGATGTTGTATTGGAAAATATGAATTTCCCTGAACCAGTTATATCTCAAGCTATTGAACCAAAGACCAAAGATGCTTCTGAAAAATTATCATTAGCATTGCAAAGATTGGCTGCCGAAGACCCAACATTTAAATACTACACCGATGAAGAAACAGGACAAACAATTATTGCAGGTATGGGTGAACTTCACCTTGACATTATTGTTGACCGTCTAAGACGTGAATTTAAAGTTGAAGTAACAGTTGGCGCACCACAAGTTTCATATCGTGAAACCATTACAAAGACTGCCGATGTTGAAGGTATGCACAAAAAGCAATCTGGTGGTAAAGGTCAATATGGACACGTTTGAATTAAATATGAACCAAACCCAGATGGAGGATTTGTATTTATTGACAAAATCGTTGGTGGTAAGATTCCTAAAGAATATATAAAATCTATTGAAAAGGGACTACGCGAAAAAATGGCTATTGGTATTTTAGCAGGATACCCTATGATAGATATCAAAGCTACTTTATTTGATGGATCATACCACGAAGTTGACTCGTCTGAATTAGCATATAAAATTGCTGCTTCTAAATCACTTACAAAAGGCCGTGAAGCCTTGGGAACAGTATTACTAGAACCTATTATGGATGTTGCTGTTGTTGTTCCTGAAGACTTCTTTGGAGATGTTATGGGTGATATTTCTAGAAGAAGAGGGCAAGTTAGAGATAACGAAACAAGAAACGATGGAGCTCATGTTATTAAAGCATATATTCCATTGAGTGAAATGTTTGGTTATGCAACTCAATTACGTTCAATGACTACAGGTCGTGGAACATACCAAATGTGATTCGATCACTATGAAAAATTACCACGTAATTTATCAGAAGAAATTATTAAAAAACGTGGTGGAAAATTAGTAGCTGACGAAGATTAATAATATTAAAAAAGATGCTGCATTTTGTAGTATCTTTTTTAATAACTAACTTTAAAAAATTTATATAATTGTTTTATGCCTTCAATTAAAAAAAATACTAATTTAAAAGATTATCAATTAAATAATAATAAAAAATTGCTAAAAGCAAGACATCGCCTAACGTTTTGATTTGGTATAGTGATTTTATGTTTATTTGTTATTACTTTTGTATGTTCATGAATTAGCGATGGACATGAATTAAAAGTTGCTTTTTCTAAATTGAGTCAAGAAACTCGAGATAAATTAATCTTAGCTAATGCCTTGCCTTCTGTTTCAGGTGCAATGTTGCAAGTTAGATTTACATTTACCTACATTAGTAACATTTTAATAGGTTTTGCATTGATATTTTATGGTTTACTTCCTAATTATCTATGGACAAAAAGAGGATTTTTTTTGGCAACTGTTTATATAAATATAACATTCTTTACTTTTTGAGGTTTAATAATACCTTTTGTATTCATTAATCCAAATATATGATATGAAATTCCTTGAAATTGAAAAGTTTTAACTGTACCAGTTCATTTAATAAATCCTGTTATAGGAACAACATTTTTTATAATAAATAGACGAGAATTAGTAGTCTCTCACAAGACAATGCTTTTGAGTTCAATTTTTATGCTTGGATATTATTTATTTGCAGTCTTAATTTTTTGAATAGGTGTGCAAGTAACTAATTTATTTTTAATTGAACAATTTAATAAAAAAACAACTGGCCTTGATATTTTTACAAAAACTAAGGTGGCAATCTATCCCTTTTTAAATTTAAAATATCCTCTTGGCTATCATGGCAATAATTTATTTTATAAAATTTTATTAAATTTATTTATTCCGATATTTGGAATTATTGTATGTATATCTGTTGCTTATTTTTTAAAATTTATTTGTAAGATTCAAACGTATTCAAAAAAAGATTTTGAAAATCCTGCGTTTTTAAGAGAAAATAAGCTCTTAATAAAATCAATAAAATCACAAATTTTTAAGTAATAAGGAAAATCATGACGGCTTTTAATTTAAAAAAATATTTTAAGTTACAAACAATTGAAATCAAAAATAGAATTAAGCAATTTAACAATAAACTATATTTAGAATTTGGGGGCAAAATTTTTGACGATTTACATGCTTCCAGAGTTTTACCTGGCTTTTTAAGCGATAGCAAATTACAATTATTGTTAAAATTAAAAAACGAAGCAGAAATTGTAGTTGTAATTTCTGCTAATGACATTGAAAACAATAAATTGCGTGGTGATCTAGGAATCAAATATGAAGATGATGTATTAAGATTAATTGATAACTTTAAAAGTTACGATTTAATTGTAAATAATGTAGTTATTACCCAATATCAATCAACCTTTAGAGTTAATAAACTGCAAAACATTCTTAGTGAACATAATATCAATGTCGTTAAACACTATAAGATTGACGATTATCCTAATAATTTAGATTTAATAGTTTCAAAAAATGGATATGGCAAAAATGAAACTATAAAAACTCAAAAGCCATTAATAGTAATTACTGCCCCTGGACCAGGCTCTGGAAAATTAGCAGTTGCATTAAGCGAACTTTATCATGATGCAATAAACAATATAGATTCTGGTTATGCAAAATTTGAAACATTTCCTGTTTGAAATTTAGCAGTTGATCATCCATTGAATTTAGCTTATGAAGCTGCAACAACAGATTTAAATGATAAAAACAAAATCGATCCTTATTATTTAAAAAGTCGCAATAAAATAGCAACAAATTATAACCGCGATATTCAAGCATTTCCAATTTTGAGTCAAATAATGAACAAAATTCAAAAGCGTAATATTTATGATTCACCAACATCAATGGGAGTTAATATGGTTGGATTTTGTTTTAATAATGAAGATGAAATTAAACAAGCAGCAAATAAAGAAATAATTCGTCGTTACTATCAAAGTGCTGTTGCTTTTAAAAAAGGTATGGTTTCTAAATCAGTATTAATAAGAAATGCAAACTTGATATCTAAAAATAATATTAATTTTTCAATTAGACCTTGCATTGAAATTACTAACAAATTAGCAAACCAACTAAATATGAATATCACAACCTTTACCTTAAACAACAATATAAATATTACAGGTAAGACATCAGCGCTTCTTGGCTCATCATCAGCTGCGTTATTAAATGCATTAAAATATCTAGCAAATATTCCTAAAGAAATTCATTTGCTATCAAAAGAAGCAATTGAATTAGTTCAAAATCTTAAAGTAAATGTTTTTAAAAGCAAAAATCCTCGCTTACATATGAATGAAACTTTAATAATTTTAGCAATGTCTGCTAAGAGCAATCAATATGCAAAATTAGCCTATGATCAACTAAAAAATTTAGCAGGTATCGAAGCACATTGTAGCGCAATACTTTCTATGCAAGATATTGAAACATACAATCACTTAAATATTAACATTACTCAAGAACCAAAATACGATAATGACACCCTATATCATAAATAGAATATTAAAAAAACATCAAGTTGTATTGTTAACCGCTTGATGTTTTTTAAATATTATTTTATATGAAAATCTAAATCAAAATCATTTTCTATTGCATTACATTCGATATTAAATGCAGCAAAAATTTTATTTGAAGCCTTAAAATCATCTGAATCAGGCTTATATGCAACTGCATAAACAACTTTAGAAATCTTAGATTGGACAATTAGCTTAGCACAGTGGTAGCAAGGTGAATGTGTTACGTATAATATAGATCCTGGTATAATCTTTGAATTTGTAATATTTGCATTTATTATTGCATTTGCTTCGGCATGGACAACGTAAGTATATTTGGAATTTAAGACATCGTTTTGGGTCACAGGTCTATTCCAAGTGAATAAATCATCATTGTTAACTTCTTTATTTAAATAACTTGTTGGCATACCATTATATCCTAGCGATATTACATAATTATCTGGGCTTACAATACAAGCTCCCACCTTAGTTAAAGGATCTTTTGAACGTAATGCACTTAATTTGGCAAGCGACATAAAATAAATATCCCATTTTAAATTATCTTTATGGTTGTTAAAAATTAATTTTCTTGTTTTATTATTCATAATAAAATATTTTAACATAATAAAGACAAAAACAGGCCCCTCAAGTCCTGTTTTTAATTATTCAGTGAAATAAGCTATAAGCTCTGTTAAGACATTAACTATGTTAATCCAATTTACTTGAAATTGAATTGCTATAATTATAATAAAAAATTAAAAAAATTTTTATTTTTTGCAATATTTTGTTTTTAATTGCTAATTAATGCAAAAAATACACCTTAATGTTAAAAAAAGTATACAAATTTAAACATAAAAGCTTAAATTGTTGTAAAATAAGTCTATAAAAAAAAGAAGGAAAAAGAAAACATGAATCAGAGTTTGAAAAACCAAAGAAACTTAAAGAATATGTTTAAACAAGCTATGTCTGGCTTTTTCGCTATATCAATTATTAGCTTTATTATGAAGAATGTTATGAATTGCGTTATGTCTAAAAACATCAACTATATTATGACAAACATCATTTGAGGAGCGAATAATGACTATCAATTTTTCAACTATAAATAATTTTTTTAACAATCAATATATTAAGACAAAAAATAATCTTAAAAACTGAATAATGGCTTTTAGAGGTTCATAGCTTCGTTTAATATTTTTATATTTTGTATTTGATAATATAAAAATATTAAAAACATAAATTTATTAAACTAGCTAATATTAATATCAAAAGCCAAATATTTCAAATATTAAGTTTATTATTCAATTATTTAATAAAATTACTAACATTTTTAATTACTTAATGACCTTTAAAATTAAAAATGTTGGTATAAATTACAATAACATTCTTTAAACAATTATTGTTTAAATATTTACAAACAAGATATTTGTATATCAACAAAGATATGCAAAACAATATATAAAAATTTAAAATTTTAAGGATAAAAATGAAAACTAAATCAAAATTATGATTACTTGGTCTTGGGTCCTTACTAGCAGTAGGAGTACCAGGATTAGTAGCCGCTTCATGTAAAATAGACCCGGCATATGTAAAAGGCGAATTCGTATATGAATTCAACGCAGTAAACAGAGAACCTATATTTGATGCAGACACTTCAAATTCATATGGTCAATATATAAACTCATTACCTGCAACATATACAGGTAGTGAACTTGTTAGAAAACAAGCCTTAACTCAAACTAAAACTATAACTATAACAGAAAAAGGCACTAAAAAAACCACTACCTATTTAGTAAAACCTACTTTTGCTAAATATAGATTAGAATTAGCAGAAGCTATTGTTTTAACTGAAAAAGGAACTAATAAACAATATGTATTTGATTCAGATGAAATTCCAAGTGAATCTGAATATCCACAACCAACTAAAACCGCAACAGACGAAAAAGGCAATGAATACAAAGTTTATGAAAAAACAAACGTTTTTGTTAAAACAAAAGGTGACAAGGATGTTGTATCAAGATCAATTAATAGTGCATTCTTTTTAGACAAATTAGCTAATGCCTCAAAATTACAATTTGTAATTAGAAAAGGTGTTAAATGAGTTGATCACGACGGAAAAGACACTGGATATGAAGTTAAAGCAAAAGACTTCTGATATTCATGACTAAGAACATATTCAAGAAACTTAGACTATCGTTTAAGCACCGGTGGATCAAAAGAACTAGACGAAGCATTAAAATCAAAATTGGCTGAAGAAAATTCACCAGTTTATGGTGAACAATGATCATTTGGTAACATATATACTTATGCATTGTTCGGCATTGATGCTAATAAATTATTTGAAAAAGATAAATTTATTCAAAAAGTATCAGCTGAAGGCAATTTAAAAGACAGAGAAGCAATTACTTTCGAATCAATTACTGGAAAAGAAAATGATGCCAACATTTCAAGCTTCTTTGAATCTCAATTTGCATCAGGAACATATGACTTTATGCCAGCTCCATCTGACTACATTGATGCATTAAATGCTAAAAATGAACAAGTTGTTTTAAAATCAAATGGTAATGCTTTATCGAAATCTGAAACAGAAGCATATCGTAACGCTTTAAATTCAATGGATAAAGAAAGCTTACAATACAAAATTGGTCAATACTGATATGGAACATCTGCTAAATCTACATTATACTCAGGCTACTACTACAACAAAGGTATAGAAAACCAAATCCAAAAATTACCAAAGAATACACTATACTGAGATAAGAAATGAGTTGAAGATGATTCAACAATCAAAACAATCGTTTTCAACTTCCAATCAAAAGCAGTTGAACCCGCATTATACAACCAAACAGCATTTAGAAAATATAGCCAAGGAACAATTAGCCAAATTGGTTTTAGTAGTTTAACAGAACCACAAAAGAAAGAAGTTTCTTCAGAACCAGAAAAATTTGGATTGAAATTAAATGAACGTTTGAATGTTCAAACTTCAATCTTTGGATTTGTTAGAACACCATTCATTTCTTCAAAATTAGGCAAAAATTACAAATTCAATGAAAACTTTGCTAAATTAGTATATGGATCTTCATTAGCAGATCTTGACAGTGGAAAAGCAGACACTTCAGAATCATATCTATTTGGTAAAGGTTTAAGCTTCAGAACACTTCTATATGCAGCAGTAAACTGAGATACATATGCAGCCCAAGCATCTTCTGGCCAAGCAACTCACTGACTTGCAAAAGTTCCAGAAGGTTCAAATTTAGGTGGTTTAGATCAAGGCACTGCAAACTTGAATACCATTGTAGACTTTAGAGACCAAGCTAACTCACTATTTGCAATTGACAAAGATGGAAACAAAGTTGATTTTGGAAAAGAATTAGGTAAGGAATTAAGTCCTTCTGAAAACGCTAAAGCTTCAGAAACTGAATCATCAATTTCTGAAAAGATGAAATCAGCAGGATTTAAAGTTCTTCAAACAAAAATGAAAGAATTACTAGATGAATTCTTTAAAGAACAAAACATTACCGATCCTGAAAAACAAACAATTGAATTTGAAGTAGGATTTAGATATTTAAATGCTCCACAACTATACATTAATGCATGAGAAAAAGTTAAAAACGTTTTTTCTGAACTAGATCCAAGAATTAAAGTTACAGTTAAGAGCTTTGATGACCCACGTGATCCAAACTTCGACGCATATAGATTTGATGGTTTGACAGGTGAAAACTTATTAGCATGATCAGCAGACTATAACGGTGTTGCTTCAACATACGACGGACTTTCATGAGGAGCTGCATTAGTTCCAACATTGGTAAAGGTTGCGCACCAAACAGGTGACCAAAAAACTAAGTTCAAACAAGCCTTCCCAGAAATTGCTAAATTAGCTGATAAATTGTTAGAATATGCAAAAACAAACAAACCAAATTTATCATTGGATTTTGAAAAAATTGATCAAATTGACAATAGATATTTAAATTCAAACTTACCTGCAATTTTAGCAGCATATAAATTAGAACAAGATACCAATACTAAAAAATATGTAATTGCTAAAAAAACTGTTAAAGGTTCAAAACCAAAACCAATCGAATTCGATTTTGGAAAAGATAGTTCAGGAAAATCAATAGAACCAACCGATCTATATCAATGATCTGCAATATTCTGAGCAAGCACATTTAAGAACTTTAAAAATGAAGAAATTTCTAAGATTGTAGAAGAATTTACAACATTTGTAACCTTAGGATATGCTAGTGACGTGTTTGTTGGCCGTGATAAATTTGTTAAAACTTTAATAAACAAACACTATGTCTCACCAACTATTGCAGGATCAGCAGTTGATTCATACCAAGACTGAAGAATAATCAAAGATACTAAAAAATAATTAATTCAATAAAAAATGACAAAATATATATTACAAAGAATAGCGTTTGCTATTCTTACTCTATTTATTATCTCTATTTTCGTTTATGTGCTGGTTGCTGCATTTGGAAACAACCCAGTTAATGACTTAGCACAAGCACAATTTCAAGCAAGCAAAAAATCAGGATTAACATATACTGAAATATTGCATAAACTTGAAATTGAAAACGGATTAAGATATAAAGATAACCCGGACATTTTAGTTCCTATAATTGTTAGATATGGCAATTATATGAAAAACATTTTTACGAAGGGCGATTTTGGATTTTTATATAATCCAGCTAACAACCCAAACTCTGCTTTATATACAAATATGGCAAAACTATTCTTTACCCCCCTACAATATTCATTAATAATCACTGTTCCAACATTCTTTATTAGTTCAATATTGGGTATTGCAATAGGTGTCATTGCCGGATATAAGCGTGGAAAATGATTTGATGTATCTTCAAATGTTTTTGTATTAATATTTGTTGCTATACCATCATTTATATTGGCTCCTATTGTAATAACTATTTCAGTCGCATTAGGAATTTCTAGCGTTGTGCCTAGACCTGGTGAACAATCATTCGGAGTAATGTTTGTTTCTTATTTACCTCCTATATTTGTTATGTCAATAACCTCATTAGCAGGTTATGTAACATATACAAGAAATCAAGTTATTACAGTATTGACATCAAATTTCGTTTTAATAGCTAAAACAAAAGGATTGAGTTCTTCACAAATTTTCTTTAAATATGTTTTAAGAAATATTTCAATTCCTTTATTTAGCCTAGTTTTTGGGTCATTTATTGGTCTATTATCAGGATCAATAATTATTGAACAATATTGACAAGTACCAGGAACATCACAAATAATAGTTAATGCCTTCCCTACCGGAGAAATTAATGTTGTTATGTTCTCTACATTATTCTTTACATTTATATCGCTAATTGCCGATATTATTATTGACGTTTCATATGCAATATTAGATCCTAAGATTAAATATTCTACTAATAGCAAGAGAAACTATTGATTATTTTTCTTGGCATATAGAGAACGTTCTAAACTTGCAAAAGATTTGTTTAAATCACAATTAATAAACAAAGCAAGTCAAAATACAACTGTTAAAGGAGATAACTAATATGAATATAAATGAAGTTAAAGCCTTTAACAAAGAATATAAAATTAATACTGAATTAGAGAAAAAATTTAAATTTGTTGCACCAGAAGATAGAATAGTAATCTCTTCTGTTGCAGGTAAGCCTAAGAAATTAGGTATAGAAATTTTAAAGAGATTTTTTACTAAACCAGTAGTATTAATAGCACTAGCAGTATTTATAATTATTTTACTGTTATCTATATTAGTTCCTGCTAATGGAATTTCTTACTATAAACCAAACGATCCAATTTCAGATAATGCATTTGTAAAGAATTTACCTCCTGCATCTTCTCCAATTGTTACTCAAGCATTAGAATCAAATGATCCCGTATTCAAATTTTATCAATTTGTCTTAAATAGGTCAGAAACCGATCCTGTATATAAGGCTAGAATGGCATTTTTCTTAAATGGCTTTCAAGCAAAAGTTAATGATGTTGGGATCTATGTTGCAAAATATAATGCTTATGATTTAGTAGTTGCTAATTACATAAATGGAATTATTTCTAAGAATCAAATAACAGATGCTGAATTCTCAAAAATATTTAATGAACAAAAAAACTTCTATTCTCATGTCTTATTAGGAACATCATCAATAGGCTACGATATTTGAACTACCGCATGATATGCAACATGAAGAGCAATTAAAATTGCTTTAATAGTTGTAATATTGGAAGCAATTATTGGGATATCAATAGGAGCATTTTTAGGATTCTATGCTGGAAAATTAATTGATACCATTATAATGCGTGTTATTGAAATATTCCTAGCACCTCCTACATTAATATGAATCTTATTATTTGTTTCAATTATTGGAGCAAATGAATGGTCATTAGTTTTGACGTTAACAATAGTTGGATGACCAGGATTTGTTGGAATAACTAGAATGTTCATTATTACAGTAAAAGATGAAGAATTCATCATTGCTGCAAAAGCAATTGGAGTTGGTACACCAAGAAGAATTTTCGTTCATGCATTACCAACTATAATTGGAAAGATTGCAAATAATATAGTAAGATCAGTACCAGGTGTTATTCTATGAATAGCATCACTAGCATTCCTTGGATTCTTTAAAGAAAAGAAGGATACTAACCTAGGACAAATGTTGATTGAAGCTTCACAAGAAGTTGGCTCAAATATTTGGATTATTGCTTTGCCTACAATTATTTTATTGTTTTTATCATTATCATTGAATTTCATTGCCTTAGGTGTGCACGATGCACTTGACCCACGTGTAATGAACAAAGGAAAAGGTAAGTAAAATGGCAAAAATTATTAATAAACACGACAATTTAGAAAAAAACAACTCAGATACTATAAAACATCAAAAAATTAATAAAAGTACTAGAGTTTTAGATTCAATTCGTGTTGAACCTCTAAAAAATAATCAAATATTAAAAGTTAGAAATTTACATGTTAGTTTCCCTCAAGGTCATAAGAAAAGCATCCATATTGTTAGAGGTGTTGATTTAGATGTCCATAGAGGCGAAATAATTGGCCTCGTTGGAGAATCTGGTTCTGGTAAGTCAGTAACTTCAAAAACCTTAATTAATGTTAATGAATCAGGTATTATAAGTGCTGATCAAATTCAAATTGGCGAATATGAACTAACAAATATTAAAAAACAAAAATATTGACAATATATAAGGGGACAAAAAATTGGTTATATTCCGCAAGACCCCTTAACTAGTTTAAATCCAACAAGAACAGTTGGAAAGCAATTATTAGATGCGTTAAATAATAATGAAGATTGAAAAAACTCAACTTATACCGAAAAGAAAAACTATTTAATTGGGTTATTAAAAACATTTGGATTGCGTTCAGCTGAAAAAATATTTTATGCATACCCTCACACTCTATCGGGAGGTATGAAACAAAGAATTGTTATTACAATGGTTGTTGCTTTGAAGCCAGAATTAATAATTGCTGATGAACCAACAACAGCATTAGACCCAACAGTTCAAGCCAGCGTTTTGGCATTATTTGAAGATATTAGACAAAAAATGGGAATTTCAATAATTTTAATAAGTCACAATATATCTGTTATTGCAAAATTTTGTGATTATATTTATGTTATGTATGCTGGTAGAATTGTTGAAAAAGGAACCAAACAAGAAATCTTTACAAATCCTGCCCACCCATATACTTGAGCATTAATTTCAGCCATACCTGAATCAAAAGATGAAAAACTATACACCATTCAGGGAACCCCTCCTGATATGGCAAATTTACCTCTTGGTGATCCATTTGTATACCGTAATGACTACGCATTAGAAATAGACTATTTAAAAGAACCTCCTCTAATTCCAATTACTGAAACCCATGCTGCCGCAACATGATTATTATCTCCTGAGGCACCAAAAATTGAAAAATCAAAAGAATTACAAAAACGTTTAGACTTATTTAAGAAGGCATTTAACAATGAATAATAAAAATTTTAAAGATAAAAAAGTTATCTTATCGATAGATAATCTAAAAAAATACTTCGTTAATCAAGGAATGATAAATAAGGCTGTTGATGGAGTTTCTTTTGATGTACATGAAGGAGAAATAGTTGGTTTAATTGGAGAATCAGGTTCAGGTAAAACAACCGTTGGTTCAACAATACTTAGACTATATGATGATTACAACGGATTTGTAAGATTAGAAGACAAAATAATTTCAGGTAAGCGTATTAGTGAATCTTTAAATCGTTTTTTAAGAAAAAATGTGCAAATGATTTTCCAAGATCCACATGCTTCATTAAATAGTCAACAAAATATTTATTCAATTTTAAAAGAACCTTTATTAGTTAATGGAGTAATGAAGGACAAAATTAAGGATATTTTTAAAGATTGATTAAATGTTAAAAAGAATTTTAAATACACATTTCAAATCGAAGCAATGAGATTGGAATTACAAAATTATTTAGAAATTAACAAATTAGCTGAACCTTTATTTAAAAAGTGAACAGAAAACCTAAATAAACTAGAATTTAATTTTGACATTTCTAGAGAAGATAACTTTAGCTTATTCTTTGGATATTTAGAAGAAAAGCAAAACGTTGAAAGCATTATTATTAATAATATGTATGCAAATGTTGATAGGTTAATTGAATTTTATTATGAAACACAACGTAAATATCGTAATAAAGAATTACCTGGCCCACAAATTGCTTTAAATGAAAAAGAAGAAAAATTAAAATACATTAAATGTTTAAGCAAGACTTCAAAGATTGCTTATGAAGCTTCACTTGAATTAAAAGAAGTTAAAAAACAAATATTTAATTTAAGACATAAAATTGAAAAAATATCTTCTGAAAGCAAGAATACATTTGCAAACTATATTCATGAATCAAAAAATGAAAAGAGTTTAATTGACATAGCAAGATTAATGTCATGTGATTTAGACTTTTATTCATATAATTTAAAAAGTGAATTGTTGATGAATAAAAGGGGTCAAGTATTAGCAAAAATAAAACCATTTAATAAATTCCTAGGATTTAATAATATAAAGAAGTTAATTAATGAACTAGATGAATATATTAATGAATTTTATAAAACAAAATTAGCCCCATTGCCATATTCAAAAACATTGAAATCTGATATTAAAAATGTTTTAGAAAAAGACTTTGATTTTAAATTCGATGAATATACTAAAATATCAGAATTAGAAAAACAAAAGTTAGATTCAGAATTAAAACAATTACTATCAAGAGAAGTTGAAATTAAAAAAGTAATAAAAGAAAATCCAAATCCTGAAATTACTTCAGAACAACTTCAAGAAGCAAAAAACGATCTAGAATTAGGAAAACAAGCATATCTTGAAGGCAGATCTAAATATTTAATTTCATATAAAGCCGAACTTGAAAGCTTGTATAAAAATCTTGAAGAACAAAAGAAATTCTATCTTGAATTGCGCAAGCAACAAGATTATTGCAATAACAAATATAAAGAATTGAAACAAAAATTCTTTGAATATGTTGAACAATTACGTCAAAAAGAAAAAGATAAGATTTTATTAAAAATAGCTGAATTAAAACAAAATCAAGACAAATCAAACAAAGATAAAAAGTCATTAGCAAAATTACAAAGCAAATTAAAATCAATTAAGGTTCAATACAATACAATATTAAAAATGTACCATTCAGATATTTCATTAAAAGAAGATACATTGAAATCATTTGATATTGAAAGAAAATATCTTGATAAAGATATAAATAATATTTATGTTTTATTAGGAATTGACCACAAGTGAGTAGAAACTAATTTAAAAACAGCTGATGCTATGGCTGGAGTTGACGCTAAACATATGAAGTGGGTATGACGTAAAAGATATTTTGATAGTAAAGTTTCATATCCTATTGCAAAATTATTAATTTCTTCAATATTGTATAAAACAATAATTTATAAAGCATTGGAAGACGTTGGATTATTAAAACAATTTGCTTATCGTTATCCTCATGAATTTAGTGGGGGTCAATTGCAAAGAATTGTTATTGCAAGAGCATTGATAGTTGAACCTCAAGTTATTGTGGCCGATGAACCTATTGCCTCACTAGATATATCAATTCAAGCACAAGTTGTTAACTTGCTAAAAGAATTATGTATTAAAAAGAACATCGGTCTTATATTCATAGCACACGACCTTTCAATGATTGAATATGTTGCTGATGAAGTTCAAATAATGCACCTTGGAAAAATTGTTGAAAGTGGAAAAACCGAAGCAATATATGCAAATCCAATTCACCCATATACAATAAACTTATTTAAAGCTATTCCTAAAATTTCTAATGCTAATGAAAAATTCCAAAATGTTTCATTTGCTTTAGATTACTTAGATGAACAAAAATTCCCTAATGTTCCTGAAACATTTAAAGTTGGCGAAGATCACTATGTTTATGGAACTGCTAGTCAACTTAAACAATGAACACATAATGCAAAATTAGAGAAAGAACATAAAGAATTATAAAAATAATTTACCATTAAACTAAATAAAAAAATTAGACTCTTGACTAAAAACATAAGAGTCTAATTTTTTATTTCTTTATATAAGCTAATAATTTATTAATTTCGCTTGAAGTTAAATCTCTATATTGACCTATTTTTAAATTACTATCATCAATGCATCCAAAAGATTTTCTATGTAATTTTTTAACATAATTATTTACTAACAAAAATAAATTTTTAACGTGATGGTTTCTGCCTTCATATAGTAGAACTTCATATTTAAAAGCACTTAATTTTGTAACTTTTTGTTTTGAAATTTTGCCATTTAATAATACGTGATTTGAATTTAATAAGTTTAATTCACCATCAAGCATTTCACGTTCTAATGTAGCAATATAAATTCTAGGAATTTCAGAAGAAGGATGGCTTAATAAATTAGCAAATTCTCCATCGTTTGTGATTAATATAATTCCTGTTGTATTAAAGTCTAATCTTCCTATTGAAAATAAGCGTTCTTTTACTTTGAGATATTCGTAAATTGTTTTTCTATTTTGAGGATCACTTGCTGTACAAATTGTATTCATAGGTTTATTTAAAACAATGTATTTAAAATTATTTGCAACATTATTATTTAATTTGATAATTCTATTGTTTATTTTAATTTCATCATTTATAGTTACACATTCGCCTAATTTTGCAATTTTATTATTTATTAAAATCTTCCCCGATAAAATTAATTCTTCTGCTTTTCTTCTAGAACAAATGCCTTGCTCACTTAAAATTTTTTGAATTCGTTTTGCTTTTTCTTCCATTTTTAAAACCTTACTAAAAAGATATTTATATATAAATATATCTATTGTCGAGTGCTAAAATTATAACTTTTTTTTGAATTTATTTTTTAATTGGAGTTGGAGGAAAAATGGAATATTTACAAAATATAGACTTAAGCAAAATAAGATCGCTTTGTTTACAAAAAAAGCGGGAATTTATCTTTAATTATTGCCTTTTTGAGCAGTTAAAAGATAAACAATTAAAACAAAAAAAATCACAAACAATCAATGACGAAGAAGATGATGATATTTCAAAATTAGAAAAATTTATTAAATTATTAAATTCTGATAATCAAAAAATCTTTTTTAATAATTTTATTTATAACAATCCTGAATCTGAATGATATTTGGCATATTGATCAAAGAATGCATATTACAAAAAACTTAATTATATAGTTAATTTATTTATTGATTTTATTAACCAATAATGTTTCCTATTGTTATGCAGGCAATCAATGATCAAGAACCAACAAAATGGATTGAAAACATAAATGCTTATACAACCAAACAATCATATTTAAATAAATGAACAAATTCATTTCAAAAGGTTGATTTTAAATTACAAAGGTTAATTGAAAATAATAAATTTATAATTGAATTTAAATATTTAAATTACGACCAAGAAATAGATGATATAGATGTTAAATGTTCGATTAACAATCAATTATTGAAACTCCAAAAAGTCTTTGATTCAGAAAAACATGAATATACATATACTCTTATTTCTGAATTTAACGAAGCAACAAAAACTAAAAAAGTTCAGGATATAAAAAATATTAATTTTGAGATTTTTTATAAGGTAAAAGGTTTATTGTATCAAGTAGAAAGATATACATATGAAATATATAGTAATAACAATTCTAATAAAGAATTAGTTTCAAATAAAAATGGCGTTGAATATGATTTATGAACTAAATTAAGAATTATGTCATTGCCAGATAGAGATATTCCAACAGATATGAAACATTATTCATTTGTCGAACAAGTTAAATACAAATTTATACCAACTACACTGATGTTGGGAAAACATAATGAAAAATTATTTGATGTTGAAATATCAAAAAAAGAAATTTCTAACAAAAGTGGCATAACTAGTGATTTTTCTCAATTAATTCAAAGTGATATTAACGGTTTTAATGTATCTATTGATATTGATAAACAATTTATAAAGGGCAACTTGAATGCAAAAATTAATGATTTCGACAAAAATGAGCATAAGCAAATTTCAATAGATTCTTATTCATATTACGACAAAGTTTCTAAGGCAATAATAGCAAATAATGATAGTATTGGAGCAACAAGAGGCTTGCTTTTGCCTCTTAATTATTCAGGAAATTATTCATATTTACTAAATGTTAATTTTGGCGAAGATTTGAAAGGCTTTAAAATTAGATACAATCAAGAAATTTCAAAACCATTTTTTAATGAAAAAAGTGGCTTAATAAAATTAAGTGTAAACAATATATTTAAACAACAAAATTTTACAAAATGAAGTGTCGTAAAAGCAGAGAATATAAGAAGTTTGATTAATAATTCCAATACTTTAGAAGAATTAATGAAGGGAGGAAAATAATGTTAATTGCAAAAAAGAAAAAAATAATTTTTGGTTTATCTCTTGGATTAGCTTGCGCCGTTGGGGCGGGGGCATTAGTTGGAGTTTATTTTTTAGCATCTTCTAATTCAAATAAAAAAGCTAATAATCTAGACAATAAAAAGGTTAACAAAAAAGATTCAACAAATAACATTAATGACAACAAAAACATTAATTCTGGCAATGAAAACGAAAAAGATAACATTATTGATAACCAAAACGAAGATAGTAAAATTGAAGATTCTAATGAAGATGGCCAAGCGAATGATGACGATAAAAAGCTTAAACCAGAAACAAAGCCAATTATAAAAACAGAGATTAATTGAAGAGAAATATTTCCAGATGTTAAAACTTCAGATTATTACAATCAATTAAATTTTAAAGATGGTCAAGGATTTATTGACAAAGAAATGATTACTTATATAATAAAAGATATTTTAAATAGAATGACTATTTTAGATGGCAATGTTTCTTATGCTTATAAACAAGAGGATGATCAAAATTTATATATTGCTTTTAAATGAGAAAATAAAAGTCAAAAATCTAATATAACTTATAAATTTAGTGTTAATAGAATATAATTAATTTTATGGAATCTGAAAAAATTATAATTAATGCTATTAAAAAACTAACTAAAACAAAGTTTAATAAAGAAACCTTTATTCGCGATTTGAATATCGATAGTTTAGATTTGGTTATACTTGTTAGCGATTTAGAAGAGGCTTACAATATAAAAATAAGTGACGAAGAATTGCTTTCTTTAAAGACCATAAATGATATTATTGAATTATTTGATAAAAAAACGAATAAATAAAGCAAAAATAATGTAAAATTATATTGCTTTATATGGGTATAGTTCAATCGGCAGAACAACGGGCTTCAACCCCGTGTGTTGTGAGTTCAAGTCTTACTACCCATGCCATATCAGAAATGATTAATAAAAGTAGCAAAATTTTGCTACTTTTTTTTAATTTAAATTATAAAAAAAGTAGGGATTTTAAATCCCTACATGACATAAAAAAATGATATGGTGCCGTCAATTGGAATTGAACCAACGACCCCTTCCTTACCATGGAAGTGCTCTGCCCCTGAGCTATGACGGCATTAAAAGCAAAATTATTATAACAAAAAAACTAAGTTTTTGCTTATAATAATAAAAATAAGTTATTAATTTTATTAATAAATTAATTTAAATTTAATTATAGAGGCTATATGGAAAAGACAATGTACGATTCACTACTTTCAATTAAAAATCAATACGATGAATTAACTGAAAAATTAAACGACCCTAATATTTATAATGATATAAAAGCATATACAAGAATAGCAAAAGAAAAATCTGAGTTGCAAGAAATTTATGATGCTTTTACAAAATATCAAAACATTGAAGAACTATATTTCTTGTCAAAAGAAATTTTGGCAAATGAAAAAGATCAAGAATTAATTGAGCTTGCAAAAAACGACATTGACAATCAAATGAATCAATTGCAAGAAATAGAAGAAGAAATTAAAGAACTTTTATTGCCACAAGATGAAAATGACAAACGTGATGTTATTATGGAAATAAGAGGTGCAGCTGGTGGTGATGAAGCAAATATCTTTTCTGGTGATTTATTTAAAATGTATCAAAAATATGCAGAAATCAAAGGTTTTAAAATTAAAATAATTGATTCTACATATGGCGCTGCCGGAGGATATAGCCAAATTGTTTTTAGTATAAAAGGCGAAAAGGTTTTTTCACAACTAAAATTTGAACGTGGTGTACATAGAGTTCAACGTGTTCCTGCAACTGAAACTCAAGGACGTGTTCATACATCAACTGCAACTGTAACTGTATTGCCTGAAGTTGATGAAGATGTAAAAATTGAAATAAGACCAGAAGATATAGAAGTTAATGTATTTAGAAGTTCTGGAGCTGGCGGACAATCTGTTAATACAACGGACTCTGCAGTAAGAATTACTCATAAGCCTACAGGATTGGTTGTAACATCACAAGATGAAAGAAGCCAAATTCAAAATAAAGAAACAGCTTTAAAAGTTTTGAAAAGTAGATTGTACGAACTAGAATTAAGAAAACGTGAAGAAGCAGAAAGCGGACTTAGAAAATTAGCTGGAACAGGGGATAGATCAGAAAAAATTAGAACATATAACTACCCTCAAGATAGAATTACAGATCATAGAATTGGATTTTCAACTTCATTAAAAGTAGTTATGGAAGGCGAATTAGACAAAATTATTGAAGCATTAATAGCTGATGAAAAGGCTAAAAAAATCATTGAAGCAGGAAATTAATGATAGATAAAGAAATTTTGCTACGTGAAAAAGAAAGATATAATTTACCCCTATATATTTCAAAAAAAGAATTAAGAATGCTAAAAAAAGATGTTCCTGTGCAAAAAATAATTGGATACCAGGAATTTCAAAATGTAATTTTAAATTTAAAATATAAAACCTTGATTCCTAGATATGAAACAGAAGAGGTTATTTTAGCTGCATATCCATTTATAAAAAAAGAATCTAAAGTATTGGATTTGGGGTGCGGTAGTGGCCTAATAGGACTTGCAATTAAAAAAAATAAAGATGCTAATGTTACATTAAGTGATATTAGCCGTTTTGCAATCAAACAAACAAAAATAAATGCCAAATTGAATAATTTAAATGTAAATGTAATTCGTTCAAATTGATTTAATAAAATAACATCTAAATTTGATGTAATAGTTTGCAATCCTCCTTATTTAAAAAAACAAAAGAAATTAATAAAATCATTAAAATGAGAACCCAAAAGAGCCTTGTTTGCTAGAGACAACGGTTTATTTGCTTATAAAAAGATTTTAAAATCTGCGTTTAATTATTTAAACGAAAAAGGAATAATTATTTTTGAAGTCGATCCTTATTGCTCTTTATGGTTTAAAAATAAATTTCCAAATGTTCTAATATTAAAAGATATAAATAACAAAGATAGAATAGCATTGCTAGCAAAAAATGATTTAAAAAATTAGCACTCTTTTTAATTTTGTGCTAAAATTAAAAATTATGGAAAATAAACGTGATTATTATGAAGTTTTAGGCATTTCTAAAAATGCCACAGAAAAAGAAATCAAGTCGGCATATAGAAAATTAGCAATGCAATACCACCCAGATAGAAACAAAGCGCCAGATGCTGAAGAAAAATTTAAAGAAGTTTCAGAAGCATATGAAGTTTTATCTGACCCTGAAAAAAGACAAAAATATGATAAGTTCGGACATTCTGCATTCGACCAAAGTTCATTCGGATATTCAGAAGATGTATTTAATAGTTTCTTTAATTCTTTTAGAGATATGTTTTCTGGCGGAGGTTTTGAAGTTGATTCGAGTGATGATTCAGATGATCCTTTTTCAAGTATTTTTGGTTTTGGCTCAAATAGATCTAGAAAAACAAAGGGCAGTGATTTGCAAATGAAATTGTCAATTGATTTTGTAGATTCAATATTTGGAAAGAATGTAACTGTTCAATTAAAGAAATACAGCGAATGTTCACATTGTCATGGTTCAAGAGCAGAAAGTGATAGTGATATTGTTTCATGTCCTCAATGCCATGGTAGTGGTCAAGTTCAACAAAAAATGGGTTTCTTTTCAACAATTAGTCCTTGCAACAAGTGTGGAGGCTTGGGTAAAAGCATTAAAAACACATGCCATGTATGTAGTGGAAAAGGATATGAATATAAATTAGTGAATGAAAATATTCAAATTCCTGGCGGAATTAATAATGGCGAATATATTAAATTGTCAGGATATGGCAATCCTTCTCGCAATGGAGGATCTAATGGAGATTTATATATTATCATTGCTATTAGACCACATAAATTTTATGAAAGATTTAACAATGATATTGTAGTTTCAATGCCTATTTCTGTTTTTTCAATTATTAATGAAGATACCATTAATGTTCCTACACCATATGGAAATAAAGATATAAAATTAAATAAAAATATGAAATTAGATGGAGTAATCACTTTAGATGGCTATGGTTTTCCTATTAAAAATAGTTCTAAAAAAGGCAACTTCATTATCACATTAAAACCTTATATTCCTTCGCTTAATAAAGAAGATAATAAAAAAGTTCAAGAAATATTTAGTAACTATAAAGATGTAGAATTTCAAAAATGAATAAAAGATTTTAATAATTAAATTTAAAAATGCATTTATTAATTTAAATAATTAATTGCATTTTTTTATATTCGAATAATTAAACTTGTATAATATTTAATTATTTTTTACTTTTTAATAAAAATAATAAATCAATGTATCCAAAAGAATATATTCAAAATAGCAAAATTATTTTTCAAAGGAGCAATATGGAAAATAAAATTATGATTGTTGAATCACCAAATAAGGTTAATACTATTCAAAAGATAGTAGGTGATAACATAAAGGTATTGTCAAGTATTGGCCATATTTTAAAAATGTCAACATCAGGCATTGGAGGATTAGGTATAGATTTTGAAAATTGAGAACCTAAGATGGTAATTGATCCAAGCAAGAGAAAAATTGTATCCGAATTAAAAGAAGCAGCTAAAAATGCTAGTGAAGTTTTAATTGCAACTGACCCCGATCGCGAAGGCGAAGCAATTGCAAATAATTTAGTAAATACTCTAAAAGTGCAAGATAAATATAAAAGGGTAAAATACAATGAAATCACTCCAGATGCCATTAATTACGCAATTGAAAACCCTTTAACAATTGATGAAAATTTAGTAAATGCTCAAAAAGCAAGAAGAATATTAGATAGGATTATTGGTTTTAAATTGTCTCAATTAATCCAAAGAAAAGTAAAAAATACTCCAACTAATCCATCTGCTGGTCGTGTTCAATCCATCGCTTTAAAACTAGTAGTTGATAAAGAAGAAGAAATTGCAAAATTTATTCCAACATTATATTCAAAAATTGAAGCACATACTGCCGACGAACAAATTGCAAATTTCGTATATAGAGATAATTTAGATTTTGTTGGCGAAAATGAATGAATTAAAAGAGAAAAAGTAGATGATATATTAGCTCAATTAAATCAAAATAAATCATTGCAGGTAATAGACTTTAAAAAATCGATTCGTTCAGAGGCAATGGTTGTTCCATTTAAACAATCTATTTTGTATAAAGAATCCCAATATAGTTCAGCAACGGTTCAAATTGCTGCACAAAAACTATTTGAAATGGGGTTAATTTCTTATCCTAGAACAGATTCAACTAGACTTTCATCAATATTTATTGAAAAATCCCAAAAATTTATTGCTCAAAAATACGGAAAAGAATATATAGCAACTGAAATAAAAGGATTTTCTGGTGATCAAGATGCCCATGAAGCAATAAGACCAACAGACATTTCACTACTTGCATCCGAAGCTCAACAAAAATTTAATTTAAGTAATGAAGAAACAAATGTATATAAATTAATCTATGAAAAGACATTAATGGCTTTGATGGCTACTCCAAAACGAGAAATATATCAATACGAATTAAAAAATGGCCAATATAATTTCAAAATGTCATATAGTAAAATAATTTTCGATGGATATTATAAAATTTTTGATAAAAAACAAGAATCTGTTATTCCTAATTATAAAGTTGGAGATATTATTAAAGTTTTAGAATTTGTTAAATTAGACAAAGAAACTAAGGCTCCTGCAAGATATAATGATGGTTCATTAATTAAGATGCTTGATGATATAAAAGTAGGAAGACCATCAACATTTGCAACGACAGTTAAAATTATAAAAGATAGACTATTTGTTGAAACTAAAAATAATAGTTTATATCCTACAGATTATGGAAAACTAGTTTTAGAAAAATTAATTCAAGGTTTTCCAACTACAATTAATGAAGAATATACAGCAAGCGTTGAAAACCAACTTGATTTAATTTCAGAAGGATTATCAGATTATAAAGTCTTATTGAAAGATTTTTGAAATAATTTTAATTTGCAACTTCAAAATGTAAATGAAACAATGGAAACAGATTTTTTACCGCAAGAAATTTTGCAAGAAAAATGCCCTCTTTGTGGACATGACTTACTTTATAGATATACTAAATCAAAACACCAAAAATTTATTGGATGTTCTAACTTTCCAAGTTGTAGATATATAAGAAATATTGACAATGAAAAGCCTAAATTTTTTAAAAGAAGATATAATAAATAATTTTTATTTTTGCCTAAATAATAACAAATGTATTAAAAAATTAGATTTTATTACAAATCTAATTTTTTATTACTTATTTTTATTGAGAAATTATTTTAATTTATTCCTGAAATTCTATCAATCATTATAGGATTATAATCTTTGCCCTTGTCATTGCTTCCTAATTTGTAATTTAGACCAAAGGACTTTTTGCTATTTTCTCCTTTTGTTGAAGTAACAAGATTATATTCATAGTTTGAGTTTATTGATACAATACCTACAAATCTATATCTATTTTCTGAACCACCCCATTTTGCTAATCCTTTGAAATTTGATTGTCATCTAGGTGGAACATTTTCGGTACTGAATTCAAATGTAACATCTTTATATCCTTTAAAAATATTGTCTTTATTTCTTAAGTCTAAATCGTTAGTATTTGGTCTTCCAGTTTTTGAATAAATATCAATATTGTAAATAGTCAATGTTTTATGGATGGTTCCGTAGTATTTGTCAGTGCTATTATTAAATAAATTTTGACATTTTTTAATAAAACTAGTTTTAGCAGTTCAGAAAAATGCATCTTCTAATTTATCATCAAATTCACTTCACTTTACAGCTGGCCCTTCTAATTCATTTTGTTTGTTAATTTCTTCTTTATATTTTGGATCATCATAATTTGGCTTACCATCTTTTAATGGCGCATTGCCTTTATTATAGAATGTTTTATAAAAATCTTCAAAATGATTCATATTCATTTTTTCTAGTTGGTCAAAAACTTTATCTTGACTTAAATTATTTCTTAAATTATCAAAAAATTCATTTTTTTCTGCTTCATTCTTTTGTACTGATGTATCGTCGAAAAATTTATATGCTGCTTTTAAAATTCCTTCATATCTTGAAATTAAAGCTTTGTCTAAAAAATCTATTTTATTAATGCATTCATCTTTTGCTTTATCGTCAGCTTTTTTATTAATTAATTCTTTTTCTATTTCGTTTATGAAACCTTGAAAAATTGCCTTTATATCTTTGTTTTTGTTTGAAAGACTACCTGAGGTACCAATGTCTTTGGTATCTAAGAATTTTAACTTTTCTTCAACTTTTTGTTTTTGGGAATTGAATTTTTTAATATTATTTATTATTTTTTCAAACTCGTTTTTAGCAGGTTCTAATTGTTTTTTAGCATTTTCTATATCTTGAATTTTTGAATTTTTATTTATGCTTGAAACACCATTTATTTTATTTTCAATTTTTGTTCTAATTGAATTATATTTTTGATCCTTCAAATCTTTATTAATAAAACTTTGCAATTCATTTTTTATTTGTTCAAATTGAGAAAATTCATTACGTTTCTTTTCTTGAATTTTAGTTTCTATTATTTTTTTAATATCATTTATTTTTTGGGTTGCTTGTTTAATTTCTTCATTTTTTGAAGCATCAGATATATTTTTATAATGATCTAAAATTGTATCTGCTTCTGTAGTTCCTACTTTTTTAGCATCATCAGTTTTTATCAAATCTTCTAATTGTTTTTTGCTGTTGTAAAAATCAGTAATACCTTTTTTTGTAATCTTTGCATTATTTAATTCATCTTGCAACGATTTATTTGCATCTTGAATTTCTTTTTTATTATTAGAAAAAGTTATTGAATTTTTTTCAGCTTTTTTATCTTTTAAGTTTTTAACTATATTTTGATAATTCTTTTTAGTTTGAGGATCATTTTCAATTTGTTTTATGAATTCATCAATATTTTTTCTTGTTTGTTGTAATTCTTTAAATTTAGCATCTTTATCTTTATTGAATGTTTCTAATTTTTTAGTAATTTCAGTAACTTTGGTATCTAATGAAGATTTAGCAGATTGCATTGAAGCAGTATTGTTTGAAGCAACTGCTTGATTAGCTTTTTGAACTTCTTTTTCTAATTCAGTTTTTGCTTGTTGAATTGTATTATCTTTGTCAGTTAATTTTGCTAATAATGTATTTGCACTAGAAATTGATTTATTTAATTGTTCTTTAGTTGATCTTGCTAAATTATCAGCTTGAGCTTTGTCAGTGTTTGCTTTTGCTAAAGCTTGTTTTAATTCAGTGTTTGCTGTTTCAATATCTGATTTGTTTGAAGAATTAGTTATTGAATTTTTAGAATCTCTTTTTGAAGTTAATTCTGAAATTAATGTTGAATAATTTGGATTAGTTTTATTTGTATTTATAAATTCATCAATATTTTTTCTTGTTTGTTGTAATTCTTTAAATTTAACATCTTTATCTTTATTGAATGTTTCTAATTTTTTAGTAATTTCAGTAACTTTGGCATCTAATGAAGATTTAGCAGATTGCATTGAAGCAGTATTGTTTGAAGCAACTGCTTGATTAGCTTTTTGAACTTCTTTTTCTAATTCAGTTTTTGCTTGTTGAATTGTATTATCTTTGTCAGTTAATTTTGCTAATAATGTATTTGCACTAGAAATTGATTTATTTAATTGTTCTTTAGTTGATCTTGCTAAATTATCAGCTTGAGATTTGTCAGTGTTTGCTTTTACTAAAGCTTGTTTTAATTCAGTGTTTGCTGTTTCAATATCTGATTTGTTTGAAGAATTAGTTATTGAATTTTTAGAATCTCTTTTTGAAGTTAATTCTGAAATTAATGTTGAATAATTTGGATTAGTTTTATTTGTATTTATAAATTCATCAATATTTTTTCTTGTTTGTTGTAATTCTTTAAATTTAACATCTTTATCTTTATTGAATGTTTCTAATTTTTTAGTAATTTCAGTAACTTTGGCATCTAATGAAGATTTAGCAGATTGCATTGAAGCAGTATTGTTTGAAGCAACTGCTTGATTAGCTTTTTGAACTTCTTTTTCTAATTCAGTTTTTGCTTGTTGAATTGTATTATCTTTGTCAGTTAATTTTGCTAATAATGTATTTGCACTAGAAATTGATTTATTTAATTATTCTTTAGTTGATCTTGCTAAATTATCAGCTTGATCTTTGTCAGTGTTTGCTTTTGCTAAAGCTTGTTTTAATTCAGTGTTTGCTGTTTCAATATCTGATTTGTTTGAAGAATTAGTTACTGAATTTTTAGAATCTCTTTTTGAAGTTAATTCTGAAATTAATGTTGAATAATTTGGATTAGTTTTATTTGTATTTATAAATTCATCAATATTTTTTCTTGTTTGTTGTAATTCTTTAAATTTAACATCTTTATCTTTATTGAATGTTTCTAATTTTTTAGTAATTTCAGTAACTTTGGCATCTAATGAAGATTTAGCAGATTGCATTGAAGCAGTATTGTTTGAAGCAACTGCTTGATTAGCTTTTTGAACTTCTTTTTCTAATTCAGTTTTTGCTTGTTGAATTGTATTATCTTTGTCAGTTAATTTTGCTAATAATGTATTTGCACTAGAAATTGATTTATTTAATTGTTCTTTAGTTGATCTTGCTAAATTATCAGCTTGATCTTTGTCAGTGTTTGCTTTTGCTAAAGCTTGTTTTAATTCAGTGTTTGCTGTTTCAATATCTGATTTGTTTGAAGAATTAGTTACTGAATTTTTAGAATCTCTTTTTGAAGTTAATTCTGAAATTAATGTTGAATAATTTGGATTAGTTTTATTTGTATTTATAAATTCATCAATGTCTTTTCTTGTTTTTTCTAATTCTTTAAATTTAACATCTTTATCTTTATTGAAAGTTTCTAATTTTTTAGTAATTTCAGTAACTTTGGCATCTAATGAAGATTTAGCAGATTGCATTGAAGCAGTATTGTTTGAAGCAACTGCTTGATTAGCTTTTTGAACTTCTTTTTCTAATTCAGTTTTTGCTTGTTGAATTGTATTATCTTTGTCGGTTAATTTTGCTAATAATGTATTTGCACTAGAAATTGAGTTATTTAATTGTTCTTTAGTTGATTTTGCTAAATTATCAGCTTGAGCTTTGTCAGTGTTTGCTTTTACTAAAGCTTGTTTTAATTCAGTGTTTGCTGTTTCAATATCTGATTTGTTTGAAGAATTAGTTATTGAATTTTTAGAATCTCTTTTTGAAGTTAATTCTGAAATTAATGTTGAATAATTTGGATTAGTTTTATTTGTATTTATAAATTCATCAATATTTTTTCTTGTTTGTTGTAATTCTTTAAATTTAACATCTTTATCTTTATTGAAAGTTTCTAATTTTTTAGTAATTTCAGTAACTTTGGCATCTAATGAAGATTTAGCAGATTGCATTGAAGCAGTATTGTTTGAAGCAACTGCTTGATTAGCTTTTTGAACTTCTTTTTCTAATTCAGTTTTTGCTTGTTGAATTGTATTATCTTTGTCAGTTAATTTTGCTAATAATGTATTTGCACTAGAAATTGAGTTATTTAATTGTTCTTTAGTTGATTTTGCTAAATTATCAGCTTGATCTTTGTCAGTGTTTGCTTTTGCTAAAGCTTGTTTTAATTCAGTGTTTGCTGTTTCAATATCTGATTTGTTTGAAGAATTAGTTACTGAATTTTTAGAATCTCTTTTTGAAGTTAATTCTGAAATTAATGTTGAATAATTTGGATTAGTTTTATTTGTATTTATAAATTCATCAATGTCTTTTCTTGTTTTTTCTAATTCTTTAAATTTAACATCTTTATCTTTATTGAAAGTTTCTAATTTTTTAGTAATTTCAGTAACTTTGGCATCTAGAGATTCTTTTGCTGATTGCATAGATGTTGGATTATTTAAATTAAGCTCTTGACTTGCACTTTGAATTTCTTGACTTAATTCAGTTTTTGCTTTTTGAATGTCTTTGTCTGAATCAACTAATTTATTTAATAATTGGTTTGCATTAGTAATTGAATCGCTTAATTGTTCTTTAATTGATTTATTTGCTTCATCAATTTGATCTTTTGCTACTTTTGCCTTATCAAGGGCTTGTTTTAATTCGTCCTTTGCAGCAATAATTTCTGATTTATTTGAAGATTTAGTTACTGATTTCTTATCATCTTTTGCATTGGTTAAATCCTTAACTAAAGTTGCATAATTTGGATTATTTTTAACGTCATCTGTTAAGAAATTGTCAATATCTTTTCTTGTTTGTTCTAATTCTTTAAATTTAGCATCTTTATCTTTATTGAACTCAGTTAATTGATTTTGAATTTTAGTAACTTTGTCATCTAATGAAGATTTAGCAGATTGCATTGAAACATTGTCATTTTGGTTAACTGCTTTATTTGCATTGGTAATTTCTGCATTTAATGATTCTTTAGCTTTTACAATTTCACTATCATTATCATTTAATTGAGGCAATAATGTGTTTGCTTTGTCAATTAATGCATTCAATTGTTCTTTAATTGATTTATTTGCTTCATCAACTTGATCTTTTGCTTTATTAGCATCTGCTAATGCTTGTATCAATGCTTCATTTGCAGCAATAATGTCTGATTTATTTGAAGAATTAGTTACTGATTTTTTAGCATCTTTTGCATTGGTTAAATCCTTAACTAAAGTTGCATAATTTGGATTATTTTTAACTTCATCTGTTAAGAAATTTTCAATATTCTTTCTTGTTTGTTCTAATTCATTGAATTTTGCTTCTTTTTCTTGATTGAATTTATTTAAGATTTCTTTATATTCAATAAGTTTAGTATTCAATAAATCATTGGCTGATTCCATTGATTCTTTATCTTTTGAAAGTGATTGTTCTGCTTTAGAAATTTCATTATCTAAATTAGATTTTAAATCAACTATTTTTTGGTCATTGTCAATTAATAACTTTTTAAATTCTTTGGCATTATTTATAGTTTCTTTAAGAGTATCTTTTATATTTTGACCATCTAATTTTTCTTTTTCAAGGCCTGCTTCTTCTTTTGCCTTTATTAAAGCATTTTGTCCAGCTTTTATTTCTTTAATTGTTGAATCTTTATTTATATCATTTAAACTATTTATTTTATCTAAAGCTTTTTGTTTTATTGATTGATATTTTGCATCATTTAATTTTGTATTAATAAAATCTTGTAAGTCTTTTTTAATATTTTCAAATTCTTGAAGTTCTTGTTGTTTGGTATTTAAAATTTTTGATGTTAAAGAAGAAGTTGCTTTTTCAACTTCAATAATTTTATTTTTTATATCTTCAATTGATGATGTTTCGTTTATATTTGTTTCAATAAAAACTTTTTGTTCTTTTGTAAAATCTACTTCTTTTGTATCTTCTAAATCAAGAACTCTTTTTAATTCTTGTTTTTTATCATTAAAAATTTTTGATTGTAATTCTCTTTCGGCATTTATTTTATTTAGAAGTTCTTTTTTTGCATTTTCAAGATTTTCATTTGTCTTTTTAATTTTATCTATTGGCAAAGAATCATCTATTTGATTTTCATTTAATGCTTTTTTAGCATCAGAAGAATCGACGTTTTGACCGTCATTTGAATTAATTAAGTTTTGTAAATCTAATCTTAATTTATTTAATTTATTTAATTCTTCTAATCTTTGTTTTTCATCTTTTTGTAGATTTTCTAATATTTTTTTACTAAAAATTTCTATTTCATCAATAAGTTTTGATGTGTTTGAATGATTTTTAAATGCTGTACTATTAAATTTTTTATCTTGTCTTGCAATAAAATCATTTGCTTTCTTTTGACCTTCATTATCTAGTTTCTTAATTAGATTAAGTAATCTAGATTTTGCTTCGTTATACTTTTTCGTGGAGTTATTGCTTATTGAACACATAGCAGCAATGGCTGATGTTGATGCAGCAGCACAAACGATTGAAATTGACATTAAAATGTTTATTCTTTTTTTCATAATTTTTCATTATCCTTTCAAAAGCTATATTTGAATAATTTAAATAATAATTATTATTATATTTTAATAGCTAAAGAATTTGAAATTAAACAATTAATTAGTATTTTTTTAAACCATTAAGTGCATTAAATTTGTTTTGCAGAAATATTTTAAATTAAGGAATATTTTTTATCTTTTTGACTACTAAAAATATGTAAAAAATATCGTTTTAATTTTTGAAATAAAAAAATACTTATAAAAGCATAATAAAATAATGGTTTTAATATAAAATTAAGTAAATATGAAAAATCATTCTAATACTAGACGAAAGCATAGATCATTGGCGCCCTTATATTATACCCTTGGTTCAATTGCAGCTGCTGCTACAATTGCGGGGGCTATATATTTTGTAGTAAAAAACGGTAGAAGAGTTAGCAAGCAATATTGAAGTGTAGAAAAATTTTTGCAAATTAGTAAAAATATAAAACTTGATCCGGTTGTAATCAATTCAGCTTCTGCAAAAGAAATTTATGAAAATTTTATTCATGAAAAACAAAAAGTAGATGCGTTAAGAAATAAATATATTAATCAACACCCTGAGTTGAAACCATATTTGAATAATAATACTAGAACGGCATTTGATTTTAAATTTAAAGGTCCTAAAAAGTCTGAAATTAAAAAATTACTAAAAGATATGCCTGAAGAATTTGACGCTCATAATTTCATCTTTTCAAAAATTTTAACAAATCAAACATTTACTGAAATGAAATTCTTAAAAGTGAGATTTTCTAATTTGAAATTAGACACATCTGATAAAGAATCAAATAAACTAAAGGTAACATATGAAGTTTATTTGAATTATGAATATGCAACAGGAAGTTTCGAACTTAATAACGTTAAGGGCACTTCGCAATCTAAATATTATTTTAAAGGAAATCAAACTGTCAAGGTAATTAGTGATTCTGAAAAGTGAAATTTGAATTCTAAATTTGCTAATAATCTAGAAAAAAATTTAGACGATGCTAGAAAGATTATAAGAATTGGAATAGTTAAGAATGATCCAAAGTGAACACAATCAAATGAATTTAGAGAAATGATTTTCAAATGATTTAAAAGAATTTTTATAGAAAATGATGCCTTTCCTGACACATATAGCAAAGATGAATATGAAATAATGCCTTTTAATGAAACAAGTAGTGGTGATGATTATGTTAGATGAAATCCTAAGAAAGGATTAAATAATCTAACAATTAAGTTTTTTTATGTAAATAAAAAAAATCCAGATATTAAATCACCTGGCAGGATTAAGATTTTTACTATATTAAAGGTTTAGAATGGAAATAATTAATAAAGATCTAGAGGCAAAATTAAAAACGTTTCAAAAGCAAAATTATAAATTGCAAAAAATTTGTCAATATTTTAAAATAGATTTAAAAAAACTTAATTTAAATGAATTTTTAGATTTTAATAAAAATAGTGAAAACCCTTTATTGGATTTAAAACAAATCAAACAAATTATTGCAGAAAATCAAGGTGATTCCAATATCCTTGAAGTTTTAAACATGATTAAAGAACTAGAAAGTATTAATAAAGAAAAAACTACAAATGATGAAACTTATAAACAAGATCAAATTTTAAAAAAAATTTTAAGTAATTTAGAGTAATGTGTCTATAATTAGTATTAATTTAGTTATATGCATCAACACGTTACAAATGTAAAGAAAGTAGGTTATTATGAAAACACAAAAGCGTATTAATATTGCAATTGATGGACCTGCTGGTGTTGGCAAAACTATTATGTCAACAATGCTAGCAAAAACATTAGGTTATAAATTTTTAAGTAGTGGTAGTTTTTATAGAGTTGTTGCCTACAATGCATTACAAAACAGACTAGATTTAAATGATGAAGAATCTATTAATAATGCATGAAATATAGAAGATATTCATGTAACAGAAGACGATAAAATTATTTTTAGAGGTCAAGATGTTAGTAAATTAATTAGGCAAGAAGAAGTTTCTATGGCCGCATCAGCAATAGCAAAATTTCCCTTAATAAGGGCAAAGGTTAATAAGTTTATTCAATCATTTGGTCTTAAATACAAAGGAATTATTGTTGATGGTAGAGATGCAACATATAGAATCTTACCGCAGGCAGAGGTTAAATTCTTTTTGTGAGCTACACCTGAAGTAAGAGCAAATAGAAGAGTTGCCCAAGATTTAGAATTAGGTTTGAAAGCCGACTATAATGAGGTATTAGAAGATATAAAAATAAGAGATTACAATGACACAAATAGAAAATTTGATCCATTAAAAATTAGCGAAGGCAGCATTGTTATTGACACTTCAAATATGAGCATTCAAGAAAATTTTGACGTAATGTATAAAGAAGTGCGAAAGAGGCTTGATGATGAGAAATAATATTGTTGCAATAATTGGAAAACCTAATGTCGGTAAAAGTACCTTATTTAATAAAATCATAAACAAAAGAAAATCCATTGTTTATGATACGCCCGGGGTTACTAGAGATAGATTATATGAGGATGCAATTTGAACTGGCAAAAAATTTAGAGTAGTTGATACCGGCGGTATAACTATTGAAGATGAAGATTTTAAAAAGCAAATTAAACTTCAAGCACAAATAGCTATTGATGAAGCAAATGTAATTGTTTTTTTAATAGACGGCAAAGAACCGCTTACAGTTGAAGATTATTATGTTGCTAGTTTATTAAGAAAAAGCAATAAACATGTTTTGTTGGCAGTAAATAAACTTGAAGGTTCAAACGTTAATTATTATGATAATTCAATTTACTCGCTTGGATTTGATGAAATATTTCCAATTTCAGCAATTCATGGTGATGGAATTGGAAATTTGTTGGATAAAATCATAGAAAAATTAAATTTTAGTGATAAAGATGTTGAATCATATTTTAAATTGGCATTGCTTGGAAAAACCAATGTTGGTAAAAGTACATTGCTTAATACTTTGGCTAATGAAGAACGTTCAATTGTTAGCAATGTCGAAGGGACAACGCGAGACTCTGTTTCGTCTCTAATAAAAATTAACGGAGAAATCTTTGAGGTCGTAGATACAGCAGGTATTAAAAGAAAAAGCAAACTAACTGAAAGTGTTGAACATTATGCTTTAATGAGAGCAAATCAATCAATTGAAGATGCAAATTTATGTCTATTGGTTTTAGATGCAACTGAAGAAGTTAGCCATTTTTCTCAAAATGTAATCGGCATAGCCTATGAATTAAAAAAACCATTAATTTTGATTGTAAATAAATGAGATTTAATTGAAAAAGATACAAACACAATGGAAAAATATAAAAAAGACTTGTTGAAAAAAGTTAAATTTGTCGATTGATCTCCTATTGTGTTTATATCAGCCAAAGATAATTTAAGAATAAATAAACTAAGAGATACTATTATTCAAGTCAAGAATAATATTTCAAGAAAAATCAATACAAATCAATTAAATGACTTAATGGCTACTGCTCAAATGATAAGACCTGCTTCATCAATAAACGGCAGAAGATTATCCATTACATTTGCAAGACAAATAGATGGATCAATTCCAACATTTTTATTGTTTGTAAACAATGTTGATTTAGCCCATTTCACTTATCTAAGATATATTGAAAATCAAATAAGAGAAAATTATGATTTCTCTGGAACTCCTATAGAGCTAGTTTTAAGAAATAAAAACAAAAAAGAAGAGAGAGAATAACAATGGCAAATATTTCGATTTTTGGAAGTGGCGCTATGGGTACTGCTGTGGCAACTATCTTAGCAAATAATGGTCATAATATTACAATATATGGCATAGATGATAGTGAGTTAAATGATCTAAAAAATGGTAGAAATACAAAATATTTTGAAGATGTAAATTTACCAAAATTTAAAGTTACAAAAGATAAAAAACAAGCGGTTGAAGATTGCAATTATATAGTTTTTGCAATTCCAACTAAGGCTTTGCCTGAAACATATAAAGAAGTAATTAAATTAATAAAAAAACAAGTCATAATAATAAATGTATCTAAAGGCTTTTGAAAAGATTCAAATAAATCTTGCCACGAAATAATGACGCAGGAAGCCCAAGCTAATTCATTAATAAGTGGTGTAGTTTCATTAATTGGGCCATCTTTTGCAATAGACATAATTAATAAAAATATAACTGCCGTTGACGCTGTTTCTAATAATGATAAACTAAATAAAAAAGTCCAAAAATTATTTTCAAATGATTATTTTAGAGTTTATACGCAAAAAGATGTGGCAGGAGCAGAATCAGGATCAATTTTTAAGAATATTTTAGCGATTGCTAGCGGTATGGTTGCTGGGCTTGGATATAGCACAAATAGCCAAATAGCCTTAATCACTCGAGGATTTAATGAACTAAAAAAATTTGTTTTAGCTCGTGGGGGTAAGCTGAAAACGATTTTTGGATTGTCGTGCCTGGGTGATTTAATGCTAACAGCATTGTCGGATAAATCACGAAATTACACATTTGGAAAAAATTTTTATAACAAAAATTTTGATAGTTCAAAATTAACAGTGGAAGGTCTTAGATCAATAGAAATTGTTTACGATGAATATATAAAAACAAAACTATTGGATTTGCCTATTGTAAATTCACTTTATCAAATAATATACAAGAATGCTAACCCTAATGAAATAATAATTAATTTAATGAAAAGAAAGCTAAAATCAGAATAACATAAGGAATAACAATGAACAAGAAAGAACTACTAAGTAAAACTAGCAATGAAACAGGATTTGCACATGTATTAATCGAATCTGCATTTGATGAAATAATCAGAATAATTGCCGATGAAATAGAGGCAGGAAGAGAAGTTACAATTTCGGGATTTGGAAACTTTTCTTCTAAATTTATAAAAGGCAAGGAAAAGGTTCACAACATTACTAAAGAAATTATTAATGTTCCAGATAGATTGGATCCACGTTTCAAATTCTCAACAGATTTTAAAAAACGTATTAACAGCAAAAATTAATTTAAACGCCTTTTATATGGCGTTTTTATTTTAAAATGTTCAAAAAATCTAATATTCCAGGAAAAATAAGCTTTATTTCTTTACCTAATGATTTTATTGTCTCGAAAAAAATATATGATTTTTTTTCTTCAAATAGGCATTTTTTAAAATTAGCATGTTCAATCAATAAAAACTCGTTTTGAAATTTAAAATAAATTATTCAAAACGCAATTCCTTGATGCAATACAACTTTATTTAAATATTCAATTTGATGTTTTTTAATATTCTTTTTTTCAAAATTCTTTTGCTCTGTGCTTTTAACTTCAAATGCTAAAAATCTTCCTTTTCAAACTCCATAATAATCTACTGTGCTTTTTGAATAGATACTTGCATCGTTTAAAAGTAATTTATTTTTTTCTAATTTAACATTTTTAAATTTAATATCCAAATTTTTTTTATGAATTAAAGCAATTCCGTTTTTAAAATAATACTCATTAGTGTTATTTATTATAGATTCTAAAAGCATACCTCTATTATTTTTATTTTTCATGTAAAAATTACAACATATTTATGCCTTGATTTTTTCGCTATTTTTTCGCTTTTAAGTCTTTATATGTTTCGAAGTTTTTAAAATAAATATTTTTTGATTTTAATAAATATTCAACTAAGTCAAATGCCATAATCATTGCCAAAACATCGTTATGGCAATATCTTTTTAATTGAGCTTCTTTGACCGTCCATTCATTATCTCTTATTTTATTTAAAGCTCTTAGCGTTGCTATTTGCAATGCCATTCCACCATTTTTTACATCAAGTTCGTGATATGGAAAAATTTTATGTTTTAAATTTAATTTATTTTCATTTACAAAAAGCTCAATCTTTTTTATTGAATAGACATTTTTTATTTGCCCTAAATTTACAATAGAGCTTTTTATATTGCAATCCATTGCTGGTCTAAAAAATAAATTTTCTATATCAATAATCTTGCTTAATATAAATTGAGATTTTTCTTTTAAAATGTCAAATTCATTTTGGCTAATAAATCCATCATCTTTGTAATATTCCAAAATTTCTTGCATTTCTTTTATTCTAGATGCTTCATATGTGTCGTTATATACGACATAATAATCCGCTTTCTCATCATATAAATCATTTATTACTTTCATAATTGTTTTATAGTCGTAATTCAAAGGGTCATAAACAAAATCATTTGAACTATATATTTCACCGTTTTGAGTTTTTATTATAGATGTTTGACTTATTAATTGATTTCATGCTAGAGAGTGGTTAATCATTGGAACGGGCAAGGTAACGCCTTCAAAATCAAACCAAACTATTTTTTTATTAGATTCTTTTAATAATAAATATTCAGGACATTTATCTTTGTTTAAAATATCGGGTGAAATTGAAATTAAATTGTTTTCATAAAAATCTAAAATTTCTTTGGTTTCAATTGAATTATCAGTTAATGATTTAACTTTTAATTTTAATAATTCCTTTTGACAAATAATGTATTTAGGAATAACCTTTTTAACTAATAATTGGTAGAATTTGATTCCTAAATTTAAATTATCTTTTAAGTCATCCATTGTTATATTGCAATCTAGTTCGTGTATTTCTTTATTACTATCAAAGTTTTCAATAATTTTTAAAAAATCAGAAAAATTGCAAGTGTATTTACTTATCTTGCTTTTTGCATCGTTATCTTCAATTTCAAAACGACTATTAAAATTAAATGCATTATTTAAATTTAATTCTTTTTTTACATAATCAATTATTTTGTCATTTTTTATTTTTTTATTATCTTCATTAAGCAGAGGATTTTTATCAAAAATAGCATTAATTTCAGTTTCTGATAAATTCTTTTTTTTGTCAAATGTTGCTTTTGATTTTTTATAATTGCAATATTCAGTCTTTATTATTTTTAATTCATTTTTTTTCGAGTTTTTTCTTGTTTGATATCAAGGTTTCACAATGAAAATTTTATTGATTTCAATTCCTAAGGATGAAAGAACGGAATAATCATAATATGCTCTTAATAAATCCTTTAGGCTAGTTTTTGATGAATATACAATATTTCCCATTTCTTTAGTTTTACTATCGTAGTAAAAAGGCTTTGATATGCATTTTTTATATTCAAAAACAGGATTTATTATTAGCGAATTTTGCATTAAAAATTCTTTTGTTTGTAAAATTTTTTCATCGGTTGAAGATTTTTTAGAAATTATATTTACTTTATTTGAAGAATTTTTTGTTAAAAAATTTATAATTTGTTCCTCGACAAGGGCTATTCCACTATCAACCATTTGAAATTGAAAAAAATTATCCAATATTTTGTTAAATAATTCTAGTTTACCATCTGAATTAATTTTTTCAAACAGTGTTGAATCATTTTGCATATCTTGTTGAATTTTTTTTATTTTTTCAATAGTATTTAGCTTTAAAAAGTCTTTGTTTTTAGTAGAGTTATTTTCAAAGTATTGATTAAAATTTTTGTCAAGAATGTTTTCAATATCATCATTTGCAAATTCCAACATTTCATAACCGTTGTCTTCTTGATTGTCGCTTTCATAAACTTCACTAAAATCATTCAATAATTCTTCTTCTCAATTATTAAAGGCAAAGTAAGGTCTGGCGGTATTTAGAATCAAAAAATTTTTATAATTAAAGTATTTAGGCTTGTTCATATTCAGGAACAGCCTTTCCTTGATCTACTCAATATAAATATAAATTTTTCCATTCTTCTTGGTTATTACATTCAAGGAATTTTAATAAATCTTGGTCAATTTGTTCATTTCATTTTGGACTTATTCCAATTTGAGTGTATTTCTTATATCATTGCATGCAATAATTAATATCATCTTGAACTTGTTCATTATTTAAAATATATGTTCAATTTTTTATTATTCTTTTAGTTATGTCTACGTTATTTGGATTTAAATAATCTTCTTCTTTTAAAAAACAAGCAACAATACTAAATGAAGTTGCGCCAATCAAATATGTGTATAATTGAGCTTGCTTTATATAGCCAGGATTAACTCCATATTTTATTCAATTTTCATAATTTTTTTCCCCAACAGTTTTAATTTCAATAATTAAATGTCTTTCGGGACTATAGCCATCAGGCAACCCTCCAAATAGCTCGTTATCTTTAAAATAATCATAATTATATTGATTGGCTTCATATCTAAGCAATTTGGAATTTAATTTTTTTTCAATAATTTTTACTATTTTTGGTTCTAATGCAATACCTGCATCAACATATTTCCTGTTCAATATTGGTTTTCTAAAACCGCATATATTACAAAATGCTTCAAATTGGCTGTCAAATGAGCTCAATCCCATTATAGCCCCCAAAGCCGAACCTGTCATTTTTTTAAATCCTCCAAAAGTGCCAGGTTTTTTTTCTTGCATAGATTTTAAGATTTTTGGATCTACTATTAAAGTTTTATTTTTATAATCCAAAGTGTAATTTTTATTGTTAAAAAATGTTCTTTTAGGAATTATTATTTTTTTTTCCATATTATTGCCCTTCTTAATTAATTACTATTAATGTAATGAATATTTAAATTATATATTTTCAATAGTTAAAAACTAACAAAATGAGAAAAAATAGCGAAAAATTAGTTTTATCAAAAAAAATAAACAATTAATGTTTAATTTAAAGTAAATAAAAAATGTTTTTTTTATTAATGTTCTAACACTTAAAATTTAATTAGTATATAATTTTTTTTGTAAATAATGAAAAGAGGAATGATGAAAAAATACACTAAAATTTTATTAACATTAGGAGCTATTTCTACTTCTATTGTTGCTGTTCCATTGGTAGCAGCAGGATGCAATGATCCAAAGAATAAAAAAAACCCAAACAATAATGATCAAAGTCAAGATGGCCAAATTGAATTTCAAGAAGGCTTAGGGTTAAAAATTGCAAAAATCGCCAAAGATCAAGAAAATGTTAAAGTTGAAGAATTTGTAAAAGAAGCAAAGAGTGCAAAGACTCTAGATCAAATAAAAGCATTATTTACTAAATATAAAATTGCTTATGATTTAAGTGAAATTCCAGATGATGCAAAATATGAAGTTGCTCCTGGTACACACGGTCATGCCGATGAAGGACTAGTACACTTAGATATTATCCAAACAGTGAATGAAAAGCAAAATAATAATAGATTTGAAATTAAAGGTTTTAAAAAGGAAACTGTTGAAGAAACAGTCCAATTGGGAGGATATAAGTTATCAACAAAAATCCTAGATAATAAAAAAGATTTTTCTTTGCAAGAAATAAAGAAACAAATTATTGACGCTCAAGAAAAAGGCTTTAATTCTTTACTTGAAAAATTAAAAGAATTTGTTAATATTGAAGTTGATGAAAATAATAAAACAAAACAATTTGAATTTAATAAAGAAAAGATTGAATTAATTAGTCAATCTGGTCAAATTGTTTTTAAAGGAATTTCTATTATTGATATTAGTAAAAAAGTAAATGAAGTCGGATATAAAACCGAAAGCAAAACTGATTTTATAATTCAAGGCTTGAACAAAGTTGAATAATTAGTTTATAATTAAGGCAATAATGAAAAACAATATTTTAATTTTATTTAAGCACATTATTGTTTACTTTTTTGTCTTGTTTGTCATTGTTAGTATTATCTTTTTTGTTATTAACTCATTGATTGAACCACAGATAAAAAAGAATATTGCAGTTTATTCACAGATAAATTCAAGCCCATGAATTAGATATGGCGAATTTTTGAATAATTTGTTTTCATTTAGAGGTGGCAAGATTTATTCAAAAGAGCTTGCAGCAACTAATTTGTCAATAATTACATTGTATTTTATGCAATTTAAATGAACAATTTTATTTACTGTAATTATTTTTATTTTTTCTTTGATTATTGGAAATTTACTAGGAATTTGAAGTGCATATAAATTTAATAAAGCACCCGATTTTGTCGTAAATATAATTGTTAGTGCTTTAGCTACAATACCTTTAATAGTTCTTGCAATATTAGCATTAGGAACATCAATTTTTCTAGGATATCCAAGTCAATTTATAAATAATGGAAGTTTAACTTTTACTTCTATAATTACACCAATTATTATTACGGCATTTGGCACAATTAGTTTATTTCATGCAAGATCAAGAAAAATAACAAAAGAAACTCTTTCTTCTAATTATTATTTGTTTGGTATTTCACTAGGAAATTCAAAAACTAAATTATTTAAACAAAACATTATTAAAAATTTAATTATTGCTGAATTACAAGTCATTTTGCCTTTTTATATATTGCTTTTTAGTACTTCATTAATAATTGAGAGAATTTTTTCAATACCAGGACAAAGTATTTTTATTTCTTATGTATTTACAAAAGCAGAATTAAATTGCATAATGTTTTATTTTAGTTTTAATTTTTTTGCTTTATTGATTGCTAGATTCATAAATGAAATAATTCTAAATTATTTGAATCCTGCTCAAGCTATTGAAAAGCGTATAAGATGGATTAAAAGAAAAAGGTTAGATTATGAATGAACTAGATAATAAAAAAGAACTTTTTCAATTTGCTAAAAAAAGACAACAAATTCACAATTACCCTATATTTCAAAGCGAAACAAAATTATTTTGGAAGAGATTTTTTAATAAAAAAAGCAACATCGTAATATTTAGTTTTTTATTAGCATTCATTTTACTAACATTAATATTGCTATGTTTTGTAAAATTTTCTCCAATACATTCGATTGATAATAAAAGCGATTTTGTCAATAATTTACCTCCCTATTATTCTCAAATTATTACAAGAAATTTTGAAAGAGGATATCAACTTGATTTGATAAGATCTATTGAAAATCAAGAGTCAATTAGGGCTGCTAAATTAGGTATTAAACCAATATTTAGAATCTTGTATGATTCAGCAAGAGATAGCGGGGGAAGTTTAACTGTAAATACTGATATTGTTGCTTTAACATATAATCCCTACGATTTAATAAAGGCAATAAATTTGAATCAAAATTCAATAAATATCAATATCTTAAATAAAGTTTTACTTGGAACAACAGAAAGCGGAATTGATATTTATTCAAGGATATTTTGTGCATTTGCATTAACAACCTCAATTATATTATTGGCAATATTTATTAATATTTTTATTGGGTTTTCTCTAGCAGCATTTTATGTTTTTAATCGTTACAAATGATATGGAATTGTTATCGATAAAATTGCTGTTACACTTAATGCAATACCTGAAATAATTTGAATATTTTTGTTGTGTATATTTTTGGGAACAAATTGATATGGTATTCTAATTGCTTTCATATTGATTAGTTGAATAAGTTTTTATGAACTTTCAAAAAACGAAATTCTTAATTTAATTCAAAAAGAATACATAAAAGCAGCTAAAATAATAGGTTTAAATAATTTTCAAATTACTTATAGACATTTATTTAAAAATGTCCTACCTTCATTAATTATTTTAATTGCTGATCGTCTTGCAATAAATATTCTAATTGTTGCATCCCTTGCCTTTCTAGATTTCTTAACTGCTGAAAGTAATTTAAACATAGGAACAATTTTTAAAGAAGCATTATCTTCAATAAAAGTAAATCCTCAATACTTGATAATTGTTGCTGTTTATATTACTATATTCTGTATTTTACTTAAACTTTTTTCAATAGGTCTTTCAATGACATATGATCCTAAGATTTCTAAAAATTATTAATAGAATTTAATTAGTTTTATAAAATTCTTGTTATATATCGTTAATTATTTAATAATATAACGAAGCTAGATTTCTATAAGGAGTTTGAATTAGTTATGTTGAATTTTATTTATAAAAACGATTGTAAAATCATATTTGGAAAAGATTCAATTCAAAATTTAGAAGAAGAATTGAAATCTTTAAAAGTAAAGAAAATTTTAATTGTTTATAGTGGAAAATATATTTTTGAACTAAAAATATATGACGAAATAATTAAGGCATGCAAAAAATTAAATATAGAATTTATTGAAAATGGTAATGTTGTTCCTAACCCAAGAATTGAATTAGTAAGAAAATTAGTATTAGAAACAAAGGCTCAAAATGTTGATTTTATACTTGCCATTGGAGGTGGTTCAGTAATCGACACCGCCAAAGCAATAGCAGTTGGAGCGAAAAGCGATGTTGATGTTTGGAAATTTTATACATATGAAGATAAACCAAATTGTGCATTACCTATCGGAGTTATTTCGACAATTGCATCTAGCGGTTCCGAATCGTCAAATTGCTCAATTTTGTCAAATGAGAATCACAAACTTGGCATAGAATATGATTTTATTATTCCAAAATTTGCAATAATTGATCCTTGATATACAAAAAGTCTCCCTATGTATCAAATTTCATGTGGCATATCTGATATTAGTTCTCATCTAATTGAAAGATATTATACAAATATAGAACATGTTGATGCAACAGATTACATGATTGAAGGACTTTTAAAGGCATTGATGATAAATGCTAAATTATTAATGGAAGATCCAAATGATATGAATGCAAGGTCAGAAGTATTTTTAATTTCTATTTTTGCACACAATAATATGTTAGATTCTGGTAGAGTTGCTGATTGAGCTTCGCATAGAATTGAACATGAATTAAGCAATTTTTATGGCATAACTCATGGTGAAGGTATGGCGATTGTCATGGTTGCATATGCAAAATATATGGCAATTGAAAAACCTAAAAAATTAGCTCAATTAGCCGAAAGAGTTTTTAATGTAGACAAGGGTTTGAGTCAAGAAGAAAAGGCTAATTTATTGTCAAAAAAATTAGATGAATTTTATAGAACAATAAAAATGAGAACTAGATTATCAGAATTAGGAGTAAGTTGCGAAAAGTTTCGTGAAATGGCATTAAGTGCAACTAAAAACGACAGTCAAACCATTGGTCATTATAAACCCCTTTTATCAAAAGAAATAATTGAAATTCTAAAGATTGCAAAGTAATAATTAAATTTTTATATTTTATAAAAAAACATGCCTTTTTTTAAAGCATGTTTTATTTTTTTACTATTGAAGTTAAAACTTTTTCTACAATATACTCTTCATCATTTTTTTCAAGGTTGCAAAATTTATTTAATGCAATGTTATATTTAGCAATTTTTTCATGAGATAATGCCTCATAAGATTCAATTAGTATTGAATTATTTCTATCGTAGTTCAGGCTTTCAATGTTTCCAATATTTGATATTGAAATAATAGAACTATTTAACCTTCTAGCAATAAGCTCTGCTATTCTAAGATCAGATCCTGCTGCTATAATATTTGTTGTTTGACTAATTTTTCTAATGTAATATGGGCCATCTTCAAATTCAACTTTTGAAGTATCCTTTATTTCTTTAATTTCTTTATTTTCTAAATAAATTATTGATGACTTTTTTGTGTGATTAAAATGAAATTCAAATGCTCCTTTTAATTCATTGAAATCGCAAGGCCTCAATAAATTTATATGGTCATATGAATATAATATTTTAAGCTCATTGCTTAGGCTAAAATTATTTCTATTATTGATTAATAGTATAAGAATTTTTGCATTTGAATATTTTAAGACTTGTAATGTTGGCAATAAATAACCTAAATAATTAATATCTACAATGACAATTGTTTCTATGCTTGAATGCAAACCTAATCCCATTGCCATATTAATCATTGTAAATTCTCTATTACCTAAGAAAATTCCTCTTCCAAGATTGTTGCTTGCAAAAATCCCATTTAACGATTTAATATTTTGAAAATAAGAAATATCATTTGATAATACTAGTAAATTTTTGTATTTATCACATAAATTATTTATTATTTTTAAAGCATTTTCAATGTATGTTTTTTCTTTAAATTCTTCAACATCGTTAATATTTTCACGAATATTGTTTTTTAAAAAGATTTGCAAATTATTTGAATTACTAAATGTTTTTATTTTGTTTTCTATTTTTTGCAATCTATTTTCATAAAACTCAACAACCTCAGAATACAAATCACTCAAATTTGTTTTTTGAAAAGAGGTTTTGTCTTTGAATTCTTCTAATTTTTCATGAGTTATTGAATTATCAAAGTTCTTTTTAAATAATTGTTCGCTTTGAGGACTTAAAATTTTAATAAACACAGGTTGATTAATTTTTTGTGCTTTTTTTATTGCTTTTATTATTGAACGACATTGACCATTTGACACTTCAATGTATTTGAAATTCATCGACTCAACTTTTTTTGCTATATTTTCTGGATTAATACTTTTACTATCTACTGTGTATTCAATTTCATTTGCATCATAGATAACAATTAATTGCCTTAAATTATGAGTTCCAGCATATGCTAGTGCTTCTTGAGTAGATCCTTCTTGAAGTTCACGTTCAGTTACATATCAATAAGTAAAGTGATTGATATTCTTATCTAACCTATTTAAATAATTTTGAGCGATTGTGTTTCCTAGTGCAAAACCGCCTGATATTCCCAAAAATATTTCGCCGCTTTTAATTCAATCTAATGTTCAATTCGAATATGAAATGTTTTGATTTCTATTACTTATTAAGTTAAGGTATTCATTTTCACTAATTAAACTATATAATCTCAAAATTTCATAAAAAAGTGGCAATATGTTGGGATTAAAAAAAACAAATCTATCCCTATTGACATCAGAATTATTTTTTCTATTTATATTGAAAAAATAGCCAAAAAGAGCATGAAATATTTTTGCCCCACTCATGATTGAAGAAAAATCACTATATTTTCAACTAAATGCCATATTTAAGGAATTGATTTTTATATTGTCAATAGCTATTTGATCAATTTTTTTATTTTTCATCTTCATAACCTTGGAATATTAAGTTAATATTGTTTGGTTTGGCATTTATTAATGAAAAAACTTTATGTTCAATCTTAAATATTAGTTTTTTTGTTTCATATGCAAAACTAGTTTTGTTTATCAATTTGTAACTTAAATTAATAAAAACATTTGTTTTAGTGCGATTTGGTTTAATTTCTATTTTTCCAATTAATTTTATATTTTTATTTGAACTAAAAATATTGTTTAATTCTTCATATATTGCATCTTGTTCAACAAAATAACGAAAGTTCATTTTTGTATTAACTTCAATTGCTCACATATTTTACGCTCTTCCAGTGTATTTACCAGTTTCGGTTGAAATAGTAACTATTTCACCTTCTTTTATAAATAATGGTGTTTCAACAACATAGCCGGTTTCTAGAGTAACCTTTTTTTGTGCTGCTTGAGCTGTGTTGCCTTTAACTGCATCTGGTGCTTCGACAACTTTTAAATTTACATTGGCAGGTAATTCAATGTCTAAAACCTCATTTTGAAATTTTCTTACTATTACCTTTTGTCCTTCTTTAAGAAAGTTTAATTCTCAATTAAGATGTTCCGAAGGTATTTCAATTTGTTCGAAAGTTTCTTGATCCATTAAAACTACAACGTCTCCTGCATTGTATAGATAATCCATATTTGATTTTTCAATCATTGCTGGTTCAACTTTTTCGCCACCAGTAAATGACTTAATTGTTGTTGAACCTGTTCTTAGATCTTTAACTTTTGCTTTAACATTTGCTTGGCCGCGGCCTTGTTTTGAATGCGCTGCTTCAAGCACTACATAAATATTGCCTTCTAATTTAAATGTAACTCCTGGTTTTAAATCGTTAACATTTATCATAACGTACTCCTTTAAATTTAATTAATAAGGTATTTAATCTATAAACTTAATAAATATAAATTATAATTAATTTATGGAATTTTACATTTATTATTGTTAAATTTAATGATAATTGAAATAAGGCAGGTTAAAATGGAAGATTTATTAAGAAAAGTTCCTAAGACAAAAAAAGAATTGAAGATATATTCTATATTAATCAATAAAATAGTTTCTTGATTAAAGCAAAAAATAAACTCCTCAAATTCTAAAGGTATAACATTAGGAATATCTGGTGGAATTGATAGTTCAACATTAGCGTTAATTTCTGAATTAGCTTTTCCAAATGGTTGCAAATTTTATTATTTAAAAACAGACAATGATTCATATACAGAAAGCCATATTAAACTTTTGCAACTTAAATTAAAGACAAAAATTGAAATTATTAATTTAAGTTCTTGCTTTAAAAAAATAGTAAAGATTGCAAATATAATAGACCAAGGTTCAATTGCAAATACTAAATCCAGATTATTTATGACAACATTATACGGTCTTGCATTCCAAAACAAAAATCTTGTGCTTGGCACAGATAATTTTGATGAATATTATTTGGGATATTTTACAAAATATGGAGACGGTGGATGCGATTTACTGCCTTTTGCTAACATAAAAAAATCTGATGTATATGCTATGGCGAGTTTGTTGGGTGTTCCACAGGAAATTATTGATAAAAAACCTAGCGCAACATTATACAAAAATCAATATGATGAAGAAGAATTAGGGTTTAAATACTCAGATTTTGAAAAATATCTATTAGATCCAACATCTGTTTCTGAGGAATTAATTAAAAAGATAAAGAATATTCACAAGCAAACTGAACACAAAAGAAAAGCAATCCCCAAGGGTCCAAAATTAAAATAATGTTCAATATAAAAATTCAAGCATCCTTAACCTAAATGCTTGAATTTTTATATTGTTTTTTATGCTTTTAAATTGTTTGCTTTTGTTCAATTGTCAAAAAAATTATCTAAGTTTAATGTTTTTATTGTTGAAATGTTTAGTTTAATTAGTCCTTTGTTTTCAATATTAAATAAGGGGTTGCAAATATTCAAGGTATAATCATTAATTTTTTCATTTCACAATTTAAAATTACTATTTAACTTTAAATTGATAATGCCATTAAAATGACTTGGAATGTAAAATCCTTTTTTGATTGAATTATTATATGATTTAATTTTTAAAAAATCTTGTGTTTCTTTGCTAAAAATATATGAATCATTTAGAAAGTAATTAAAAGTATAAGAAAGGGGTCCATTTTTTTCTAAAGTATATTCTAATTTGTTAGTAATATTTAATTTATTATTTTGACTATAAAAATTAATTGAATCAAAAATTGGATTTTTTAATTCTGGATAATCATAAATAATTTTTTTGTTATGTTGGTTTGTTTCAATAAAGCTTTTTGAATATCCAATGTGATTTCAGGGCATATTTTGTTTTAGTTGATTGAATTTAAAATATGTAACAAAAAATTCTTTAACTATAGTTGTAGAAGAATTATTAACATCTTCTTTAAAACTCTTGTCAGTAAGAAAAATATCAATATTTTTTAGGGTTCTTGTTTTAAAATCTGAGTCTATATTTCTATTATCTGATAGGTTATTTGCTAAGTCATAGTTAATAAGAATATTAGCCCCAACTTTATAGCTCAAATAATCTCTAATTTTTGTATAAGCATCAATTTTTACTATCAGTTTTTTTTCTAAAAATATAGGATTTCCATCTTCATATTTATCAATTTTAAAGTGAATATATTCATCATTTGTTTTATGATAAATCGACAAATTTAGAGATTTTTTATTTATTGAAATTTCTTTTTTTGTAGCAACTTCATAAATCAATCTATGAATTGAGAGAGTGATTGATAATTTATTGCTTTTTTCATCTCTATTTATTTTGATAAATATTGGATATTTTTTTAGCATCGTATTAGATACTAAAGTATTTGATTTTAAAGATTTTGAAGCAGATATTTTAAAAGTTACATCTTCACAAATATAGTTTTTTGCTAGGATATCATTTGCCTTTTTTTCAACAACTACTTGGTACAATCTTTCTTTTCTTCTTTAAAAATATTTTCTTTGAATTCACAAATCAATCTAGTTTTTGCTATAAAATACAAATAATAAAAGGGGATTCAATATTTATATAAATCGTGTTGTTTCTTTTCTTTGTCTATTTTATATTTTTCCATTGATCCTCCGGTGTCTAGAATTTGCCTTCTAGACATAATAATGTAACCATGAAATTTAAGGATTTTGCCAAATTTTGGGAATTGTGAAATCTCTTATTTATTACATAAATAAAGAAAATTTATTGTCTAAAAAATCAAGAATCAAGCAATTGCTAAAATTAAATAATTTAATTTACGATAATATGATGATTTAGAAAAGAAAAAGTCTGTTCACTTAATATCATCTTTAAGCTCTTTAAAATATTTAATATAAATACATTTCAAATCATCACTCAATGAAGATTCAAACAATTTTGCTGTTTTTAAAACATATTTTTGAATTAAGAATTTAAGTGAAATTAAATTCTCTTTATTAAAAATTTCGGGAATTTTATTTATATCTATTTTAATTTTTCGTTCCCTTAACTCTTTTATTATTTCCTTAAAATAATTAAAACATTTTAAAAAGAATGCTCTGTTGCTACTACGACGCATTTTTTGCCTCCATTTAAATTGCGTTTTGTATGATTTTATATCTTTATTTTTAGTAAATAATTTCAAAAAAAGTTAAATAAAAATCTAAAAAAAAGTAAGTTATACAAAAAAACTTACTTTTAGGTTTTGTATTTTTGATTTTATAAATATTTATCAGGATGTTCAATTTTCATTTTTTTAATAAATTCTTCTTTATCAAGAGAACCATATTTTTTCATTAATTCTTGACATTCTTTATATTCATTTTCAGCTCAAACAACATCTTCAAATCCTGTTACTAATGTAGCATTTTTACCTTTAAATTCTTTATAAGTAGAAAAGAAATTTCTTACTTGAACTAATCACATTTGATCGACATCTGCCAATGATTTAATATTATCTAATCTATAATCATCTGCGTGAACTGCAATTAATTTAGTATCTGTTTCGCCACCATCAATCATCTTCATTGCACCAATTAGTCTTGCATTTAATGATGTTCCGGGTAAAAAACTTTCTGAAGAATAAACTAAAACATCCAATTCATCTCCGTCTCAATCTAGTGCATCAGATAAATAGCCATAATTTGCAGGATATTTAAAATCACCTCTTAAAATTCTGTCAACTTTGATTTTGTTGCTTTTGCGGTCATATTCATATTTGATTTTTGAATCTTTACTAATTTCAATGTTTACTTCTAAATTTTTCATATGCTTATTTTATCACTTTCATAGAAATTCCTTATTGTTTGGGAAAAAAATGAGAGTTTTCGCTTATTTTTCCCAAATGAAATAATAGTCAAAAAAATCCAATAAACTTAAAAGTATGAGGAGTAAAAATGAAAATATATTTATATGGAAAAATAATGCACGTAAACACTAACTATTTAATTTTAGATCATAATGGTGAGGGCGAATTGATATACGTGGCGGATATAAAAAGATTTAATAAGGATGATTTGAGAAAAATATTTATTTACGATGTGGTAAATGAATATAAAAAAACTACTTATGGATTTGAAAATTTTAAAGAATTAATAATTTTTGAGGATTTAATTTCATTACAAGGTTTAGGACCTAAGACAGCAATTTCTCTTCTAAATTTAGGATGAGAAAATTTAATTCAACTAATTGCAAACGAAGATAAAGATAAATTATGTGAAGCATCTTATGTAAGTTTAAGGATTGCAAATAATATAATTTTTGCCTATAAACAAAAATACGTAAAATTTTTATCAAAATTAAGCGATGAAGATTTACTTAAAATCAAGAGCAAAAACACAAGTGACTCTTTAAGAGAAAAATTTGAATATACAATGAGAATGCTCGGTTTTAAAAAACAACAAATTTCATACGCATTAGAAAAAATGAGTATAACCGAAAATATTGAAGAATCAGTTGAAAATGCAATAAAAATAATTGGAATGAAGCAAAATGAGTCAAGAGTTCTCGATCAATAGTTTTGATGAATTTGTAGGCCAAGAAAAACTAATTTCCACTATTAAAATAATAATAGAATCTGCAAAGGTTCAAAAAAAACAAATTGATCATTTACTATTTTATGGCCCGCCAGGTTTAGGTAAAACTACACTTGCTAAAATAATTTCAAAAGAAACAAAAAGCAATATTGTTTATGTTCAAGGTCCATTGATCGAAAAAAAGAGCGATGTATTGACTATTTTTTCATCAATAAATGATAATGACATCATTTTTATTGATGAAATACATGGCATAAACAAAAATATTGAAGAATTATTTTATTCAGCATTAGAAGAGGGTACAATTGACGTTGCCTTGGGCGTTGATGGTGATAAAAAAATCATGAGAATGAAATTAAAAAAATTTTGTTTGATTGCGGCCACGACAAAAATCAATTTGTTATCCAAACCCTTAAAAGATCGTTTTGGTTATATAGGAAAATTAAGCGATTATAGCGATTTAGAAATTGCTAAAATAATTTTTAACTCTGCAACTAAAAATAAGATAAAAATTAAAAATGATGCGATTAATTTTATTGCAATTCATTCAAGGCAAACACCAAGAGTCGCTAATACTCTTTTAAAAAGAGTTAATGATTTTGCCATATATTACAAAATTGATCTAATTGACTTGGAAACTGTTAAGAAAGCTTTTTCGTATTTGGGTATTTATTTGTATGGTTTGTATTCGCCTCAAATTGAATATTTAAAAACATTGCATAAAGTATTTAATGACAAATTTTCTTCGCTTGATGCGATAAGTTCAATAATTAAGGATGATAAATATACTATTTTAAATGATATTGAGCCTATATTATTGATTTATAAGTTAATTGAGAAATCACCCCGTGGTAGAAAAATTACAAACAATGGCATTGAATATTTAAAGAGTCAAAATATAATTACTTAATTACTAAGTAATTTTAATATTTTAGATAACATGCATTTTTTAGTATAATTATTAACTATATGAAGAAAACAAAAATTTTAAACAATAAATTTAGATGAGTTATTAGCATTTTTTTAATTTTAATTTTATTGATTGTGACAATTTTAGGATCGATTTTTTATTTGTTTCCGCAACTTAAAAAACAATCCAATTCAAATGTTACATCTGCTAAAATTGGGTTAAAAATAGAAAGGGAAAATCAAAAAGAAAACTCGTATAAAAAATCATATGGATTATCAGATCAAGAAATTTTGAATTTGACTAAGAATTACATAGGAAACCAAAACGGGGTTTTAAGTACTTCATATGATATTAATTTGGTATCTAAAAATTTGATTTCTGCTACTAGTTATCAAGATAAAACAGATAAGGATGTTAATAATTTAATATCCAATTTTGTTAATAAGCCTTATTTAACAGTAACTGATGGTGGCGGTAATCCATTATTTTATAAAGGTGAATATGTTTCGAGGTTTTATCCAAATCCTGATGTTCCTAGAACTCTTAAAGATTTTTTAAAAAATGGACCTCAAAACTACAATATTAGTGTAGAAAAAAATCCGGCAACAACCTACAACAAATTTGGTGCAAGACAAAAAATTCAAGTTAAATTGGATGAATATGGATGGGATAGTTTTGTGCAAATGATAAACGAGTACAGGCTTGCAACAAGTTGATCTTTACAACGTAATAGAAAGGCTTCACGTGAAGAAATTATAAATAACCCAGGCAATAAAATTTACTTTTGAATGAATTTGGCGGAATTCGTTCATAGAGCAAAAACTGAGTTTCCAGATGAATGAAAAGCAGCGGGCGAAAATCCTATAAATTTTGCTTATGTTGGAAATTCTGCTTTACCAGAAATAAAAAAGGACAAAAAAGGGAATATCATTGATATTAAATATCCTGTTTTAAAATCAAAAGAAATTAATGCAAGAAAATATTTGATATCAGCAGTTAACCCTGCAACATCTAGAATACTTTCAACTGAAAAAAATGAATCATCATTTTATTTGGCAAACGAAAATCTTGAAGGTTTAAGTGATGAATATATTGCTGCTGCAATTAACTATTCATATGCCCCATTTAAATTAACTAAAACTTACAATTATTTTTTAACTGGTAGTTCAACAAATCAAAATAGATATTTAGCAGTTTTAGCAATTTTATTTTCATTGTTTGCAGTATTTTTGATCGCAAAATATCGTTTATATGGAATCGTATCATCAATATGCTTGGCATTTATGATATTTGTGTTTTTATCAATTATTACTTCATTTAATATATATATAACGGGAGCGGTAGCATTTACTTTCTTAATTTCTACATTCTTAACATTTTTAATGATTTGGCAAATATTAAGACGTCTAGATAAAGAAGTAAATTCAGGTTCTAACGGAATAAAAGCAACAAATAAATCATTAAGGTTTGGAATAATATCAACACTTGATATTGCCTTTGCATTAATTGTGGTTTCAATATTTACTATTTTTATAAAAACTTCATATTTAAATGCGATGGGCATAATGCTATTTATTGGATTGGTTTCTGGATTGATTATTGGAGTATTGGTCAATGCGTTAATAATGTGAAATTTAATAAAAACAGAAACGTTTGATAAAAGAACAAATTGAATTGTTTGAAATAGCAAAAGAGCAAATTCTTTGATAGCAAAGATTGATTTAATCTCAAAATCAAAATATTTTAGTATAGGTTTTGCAATATTTGTAATATTAACCATTGTATTCTATGGAATATATAGCGGTGTATTTGGAAGTACAAAATTAGGAATTAATGCTAGTGAAACATTAGCAAATAATTACTTATATTCTATTCAAAAATCATCAATAAATGGTGATTGAAATAAAACTGAAGTTGATCAAATTATTAAATATTGAAAAGAATTAAATATTTCAAATGCAAATATTTTCTTTGAAAAATCAAGTTTAACTAACGACTCATATAATTTATTAATTTCTTCAGCGGACAAGATTGATTCAACTAAATTAGAAGCTTTAAAGACATTTATTAATACTAAAATTTCAGCTATAGGTAATGAATTTGATTTGAACATACACGAAATATCATTAAATGCAAATGGTGAATTAATCAATTTTGGATTTACTATTTTAATAATTGTGGTTTCATTAATAATTTGTGGAATATATAGCATTATAAGATTTGGTTTAATAGCGTTTTTTGTAATGTGAATAAACGAAATATTAGCAGTTATAAGTTCAATAGTTTGATTAATAATTTGCCATGGACAATTTAACAATGGAATTTTAGGAGCATTGATATTATCTACTGCATTTAGTGTAGTTGATTCTATCTCTAATTCTCAAACAATTAAGGAAGAATTTGGCAATAATTTAAATACAAAAAATTACATATTTGAAAATGACCAAATAAACAACATATTTAAAAAATATGTAAAGGAATCTTTGAATAAAAACATCTTCAATATATTCTTTGCAATTATTTTTGTGGCTTCTACATATACATTGATGACGGGCTTTAATGCAACAACAATATTAGTAACTTCAATGTTTATTATTTCAAATTCATTTATCAATTTATTTATATTACCAAACATTTGAAGATTGATGTATATAAAGAGATATTCAATTAAGCAAAAGAGAATTGAAACTAAATATTGAGAAACAGAAAAGATTAGCGAACAAACATTTGTTGGTATTAATGATTTTAATATCTAAAAAAGGAGGAACCAATGTTTCAAAGACTTAAAGGCACAAGAGATATATATGGGTTAGAAGAAAAGGTTTTGAATTTTATAGAAAATAAATTTTTTAAAATTATAAAAAATTACAATTTTAAAAGAATTTGCACACCGATAATTGAAGACGCTAACTTGTTTATAAGATCAGTTGGTGAAACTAGTGACATTGTTTCAAAAGAGATGTATCTGTTTAAAGATAATGGCCAAAGAGACATTGCCTTGAGGCCAGAAGGAACAGCCTCAACCATAAGAGCTTTTGTGGAAAACAAAATAAACAATTTGGAGCCTACTAAATTAGCCTATTTTGGACCTATGTTTAGATACGAACGTCCTCAAAAAGGTCGCTATAGACAATTTATTCAAGGAGGAGTTGAATTAATTGAAAATTCAACTGTTGAGAATAATTTTGAAATAATAAAATTGGCATATGATTTTTTAAAGTCGTTAAAAATTAGTAACATTACTTTAGAAATAAACAATTTAGGTTCTTTTGAGTCAAGAAATAACTACATAGAAATATTAAAGGAATATTTTTCTAAATACAAGGAACAATTGAGTGAAATTTCTTTAATTAGATTGCAAAAGAATGTTTTGAGAATCTTAGATGATAAAGAAGAAATAAAAAAAGATTTTGTAAAAAATGCTCCAAGATTAATAGATTATTTATCTTCAATTGAAAAAGAAAGATTTAACAATTTACTAGATTTACTAGATGAATTTGAAATCAAATATGTAATAAATCCTTACTTAGTTAGGGGACTTGATTACTACAATGATGTTGTTTTTGAATTTGTTTCAAATTCAGACGCTCTAGGTTCACAATGTACTGTTCTTGGCGGCGGAAGATATGATGGCATGATTAAAAGTTTTGGCGGACCTGATATAGGTTCTATTGGGTTTGCTTTTGGAGTAGATAGATTAATGGAAATAATAATGTCAAATATTGACAATTATCCAGAGCTAAGTTTTAATGAAACGATATTAATTGCTTATTTGAATGAAGATGAAAAAAATGAAATAAGAAAAATTGCTTATGATTTGAGAAAGAATTTTCAAGTAATTTTTCCAAGTCAAAAACAATCAATTAAGAGTTTGTTTAAACTTCAATATAAATTGCAACCAAAATATTTAATTTTTAAAGAATTAAATATGAAAAAAAATGAGTTGAAAATAAAATCAGTTTTAAATGAAAGAAATATAATTTATACTAATTTAGACGAATTTAAAAATATTATTAGTGAAATGAAAAGAGATGAAAACAATGAATAAAAATTATTGTGGAACATTATGTATTAATGATTTAAACAAAGAAGTAGAGCTTTGTGGATGAATTGCAAACAAAAGAAAATTTAAGCAACAAGTTTTTATTGACTTAAGAGATTCAAGTGGCGTAGTGCAATTAATATTTCAAAATGTATCAGATCCTTTATTAACTAAAGAAAGTTGTATTTATGTAAAAGGAACTGTTCAAAAGCGTTTAGAAGTTAATCCAGCATTAAAAACCGGAGAAATCGAAGTTATAGTTTCTGATTATAAAATTTTTAATTCAGCAAAGCAAATACCTTTTGAAATTAACAATTCAAAATCAACAAATGATGCAAACGAAGATATAAGATTGGAATATCGTTTTCTTGATTTAAGAGAAGAAAGAATGCTTAACAATTTAAGATTAAGACATAAATTCTTACTTTTGGTAAGAAATTTCTTTGATAAACAAGGCTACATTGAAGTAGAAACCCCAATTTTGGGTAAATCAACTCCTGAAGGAGCTAGAGATTACTTGGTACCCACAAGAAGAAAGGGAAGATTTTTTGCATTACCTCAATCACCACAACTATTTAAGCAATTATTGATGGCTGCTGGATTTGAAAAATATTTCCAAATTGCCAGGGTTTTTAGAGATGAAGATTTAAGAAAAGATAGACAACCAGAATTCACTCAATTAGATATTGAAATGTCATTTGGAAGTCAAGAAGAAATATTTAAATTATGTGAAGAAATGTGAAAGGATGTACTTTCACAATTGGGGTACAAAATTGAAACTCCATTTCCAAAAATGGACTTTTTCTATTCAATGGCTCATTATGGAAATGATAAACCAGATACAAGATTTGGATTTTTAATTGAAGATTATTCAAAACAATTTGCAGCCAAATATAATAAGAAATTTGTAAAAGCTATTGTTTTTGATAGAAATATAAATGAACATAAACAAATAATTAATGAAACTTTTAAAAAGAATAATGGACAAATATTAGACATATTCGACTTAAAAGAAGCAAACCAAAATGAATTTCAATGTCTTTTAGATAAGGCAAAAGAAGATAATATTTCAACCCCATGAGCAATAATTTCAGCTAATGATTTAGAAGAAGATGCATTGAAATCACTTGGAGCAATTAGAACAATTGCAAATGATTTATACAAATTAGCAGATCCAAATCAATTGAATTTCTTATGATTGGTAAATTGACCTATGTTTGAATATGACAGAGAAACAAAAACATATTCACCTGCTCACCATGCGTTTACACAATTTGAAGAATCTACAATGAAATATTTAGAAACTGGAGAGCTTGAAAAAGTTAGAGCTAAATCATATGATTTAGTGTTAAATGGATTTGAATTAGGATCTGGTTCTATAAGAATTTATGATCCAAAGATTCAAAAGAAAATGTTCGACGTAATTAATCTAAGTGAAAAACAACAACAAGATCGTTTCGGATTCTTTTTAAAAGCATTTGAATACGGTTTACCTCCTCACAATGGTATTGCATTTGGAGTTGAAAGAATTTTAATGATTTTAACCAAATCAGAATCAATTAGAGATGTAATTGCATTTCCTAAAAATGCTAAAGGCATTGACTTGTTGACATCAGCTCCTAGCGATGTGACAAAAGAACAATTAGATGAATATGGATTGGAACTTAAAATTAATAAAAAATAATAATATATAAATTAAAATATATGGGTAATAGTTAAATTAATAATTTGATTTTAATTATAAACAAAACAACATAAAAAAAGAAAGGGATTTTATGCATGCTGCTTCAATAGCCTTAACAGTTATATTAGTAATAATTTCATTTGCGATAATTGGAATTTCATTTATTATGGCTCCTGATTCAAATAGTTTTAGTGGAGCTTTAGTTGGTTCAAATGACCTAGACTTGTTCAAAGTATCAAAAGAAAGAGGAATAAAAAAAGTATTAAAGTGATCAATGATTACTTTAGGTATATTACTTTTTATATTCTTGATCGCTTTAAGAGTTGTAATACAAAATGGTTAGAATTTTTAAATAAAAATGACATATATAAAAAATTTTAAAAATAAGAATAACAACCCTAAAATTAATGAAGAACAAGTATTAAAATGCATAAGAGAAAGTGATGGAATTACTTTTATTGAAATTGCAAAAAAATTAAGAATTCCTGCTTTTTTGAATTTTGAATTATCAGAAATATTAAATAACTATTTATCTAAAAAATTAATTGATACGAAAAGCGATGAAAAATACATCGCTATTTATTTTCTAACTAAAGTACAAAAGGAAATATCAATAACTGCCAAAAGATTAGGATTTATTGATTTTGAAATGCAAGAAGAATCTCAAACTAAAGTATTTAGTGCCTTTCTTGCACCTTTTCAATTAAAAGCGGTATTAGACAAGGATTTATTGCTTGCAAATATTTATTATTATTTTGAAAATGGCATAAAAAAATATAAAGGCGATATTATTCAAATTTTAAATCACGCAAGAAAAACTATTGTTGGGACTATTGAAAAGAGCGCAAATAAACTGTTTTTTAATGCTTATGATGAAAAAGATAGGGCTAAATTTGAAATTATTAATGAAAAAGAATGCCCAGATATAACAAAATTGAAAAATTCATTGGTATCTTGCGAGATTTTAAAACCAAATAATAAAAAAGTTGCTATTGCATATAAAAATATAATTGGTAATATTAATGATAAAGAACATGTGATTAAAAAAATCATTGCCCAAAATGATGTAAATGTCAATTTTCCACAAAATTTATTAGAATATGTTTCCTCAATACCTTCTTTTGTTTCACAAGAAGAAATTAATTCAAGAATAAATTTAATAGATTTAATGACAGTTACAATTGACGGTCTTGACACTAAAGATTTTGATGACGCCATTTCGTGTTATAAAAATGAAAAGAATAATTGAAAGCTATTTATTCATATTGCTGATGTTTCATACTATGTTAAGGAAAATGATTTAATAGATAATGAAGCTTTATTGCGTGGCACAAGCATTTACTTACCTGATAGGGTTATACCAATGTTGCCGGTTGAACTTTCTAACGGAATTTGTTCATTAAACCCAAATGAAGTCAGAGCTTGTATAACATTGGAATTAGAAATAGACAAGCAAGGGAACAATATATCATATAAAATTTATCCTAGTGTTATAAAATCAAATTATAGATTGACTTATAATGAAGTTAATGATTTTTATAGCAATATAAAAAGTGTTCCTGATGATGTTGCAAATATGCTGTTGCATGCACGAGATCTTGCAAAAATTATTAGAAGTAAAAAAATATCCGAAGGTTATGTTGACTTTGAAATTCAAGAATCAAAAGTTATAATGGATGGAACAAAGGTTGTTGATATCAAGGTGAAAGAAGAAGGCGAAAGCGAAAAATTAATTGAAGACTTCATGGTTCGGGCAAACGAAACCGTTGCTCAAATGATGACTAATTTAAAAATTCCTTCAATTTATAGAATTCACGAAAAACCTTCACCTGAAAAACTAACTTTATTGCAAGAATTATTCAATTTTTGTGGATTTAAGGATGTAAAAGTTCCTTATGATGGAGAACCAAAATCATTTGGAGAAATGATTGCAAAATTAAAAGAACATAAATTTGATGATTTTATTAAAATGGCATTATTAAGAACAATGCAAAAAGCAATATATTCATCAAATAATATTGGCCATTTCGGTTTAGCTTCAAAAGCTTACTCACATTTTACTAGTCCAATTAGAAGATATCCAGACTTATTGCTACATAGATTGATAAGAACTTATATTTTTGAAAAGAAAGAAATAACACCTGATTTATCAGAAACGTTAAAACAAAAAATAAGTCAAATTGCTTTAATGAATAGCGAATCTGAAAAAAATGCAATGGTTGTCGAACGAAGTGTTGTTGATGTTAGAAAATCAGAATTCTTTGAAAATTTAATTAATAAGCAATTTTTTGCAACATTAGTATCAATAGAAAAATTTGGAATGTTTTTTAATATTGATGAATACCAAACAAGTGTTTTAGTTCGTTTTGAAAATCTTGCGATTCCAACTAATAAAGACAACGATTTTCAAGCTTCATCTAAAAATAAAATCTATAAAGCAGGCAATAAATATTTAATTACTATCACTTCGATAGATCATGAAAAAGGAAATGTAAATGCAACATTGGCTTAGCATTTACATTTTTTTATTAATTAATTAAAAATAATTTTAATAGTTATATTTTTTTATAAAATAATAGTATGCCCAAATTGATAATTTCAAATAAATTTATAAAATCAAATTATGAAATTGATTCCACATATGAATGTGGCATTTCGTTATTAGGTTGAGAAGTCAAGAGTTTAAGGGCAAAAAATGCAAAACTTGATAATTCTTTTTGTTCAATTAGTAATAATCTAGAATTATGAATCAATAATTTAAATATTGCTCAATATATGCAGGTAAAAGGCGATTTAAAAAGAAGTAGAAAATTGCTAATGCATAAATCAGAAATATTAAAATTAAAAAACAAGCAAGAAAAATTTTCTTTTGTTATTGTTCCAGCTTCGATATATTGAAGCAATAATCATATTAAGGTAGAAATAGCTTTAGCTAAAGCTTTAAAAAAATATGATAAGCGAGCTAAAGAAAGAGAAAACGAAATAAAGAAACAAATAAAATCAAGTTATTAAGGGGGCGTACAGGCTTCGACATACATGCAAGTTATTAAGTTGCAGTAGTTTGGCAAACTATAATGCTACTAGGCTTTTTAAGCGCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAAATTCTGGTAGGCCAATGAATAGCTCGGTTCATTGTTAGCCTTTGGAGAGCTTAGCAATTAAAGTGGCGTTTTAATTGCAAAATTATGCCAATGGTTATTATGAAGGTTGTTAATTTGATAATAATATAACCAAACATAAAATTAACTAAACTGTAGATACTTAATAACAAACATGTGTGGACCGGGGTTCGATTCCCCGCGCTTCCACCATATGAAAAATAAAGAGATCAGCGTTTTATGATCTCTTTTTCTTTTAATTTGGTGATATTTTTTTTAAAATAAAAATAAATAATAAAAATTATTTTTATTTTTTATAAAATTAAAATAATCTAGTTATTAAAATTAATAAATTAAATATAGTTTTATAAAACTTATTTAACTTTAATATAATTTTAACTATAAATATATAAGGAGGGGAAATGGCGGTCAGATTAGTAGTAAAGCATGAAGATGGTTGGGCAGTCAAAAATCCAAAAGGAAAAAGAGCATTGAGAGTATTTAAGACTCAAAAAGAAGCTGTTCAATATGCAAAAACTTTGCAAGATACTTCATCTGTAAATGTTCAGTCTAAAGCTGGAAAGTTTAGAAAGTTCACAAGCTAAAATGGCAATATTATTAAATGGTAAAGCAATCTCTTTAGAAATTAAAGAAGATTTAAAAAAAGCTATTGAAAAAATGCCTCAAGAAATTAGACCTACTTTAGGAATTTTACAAGTAGGCGATTTAAGTGAGTCAAATATTTATGTAAAAAATAAACTTAAATATGCTTCTGACATAGGATTAAATACAAAACTAATAAAATTGCAACAAAATGCAAGTAAATTGCAAATTTCTGAAGCAATAGAATTATTAAATAAACAAAGCACCGGATTTATAGTTCAAATTCCAATGCAAACTAATCAAATTATAGATAGTCAAGAAATATTGGATTTAATAAGCATAGATCACGATATTGATGGTCTAACCTCTTTTAATCTTGATTTAAAAAATCCTAATAATTATTTTTTATCAGCTACTGCTAGTGGAATTTTTTTGCTTTTAAACAAATACAATATTCTTTATAAAAATGCAAAAATTGGAGTTATTGGTCAAAGCAAAATTGTCGGGTACCCATTGGCAAATTTCTTAGTTTTAAAAGGATATAACGTATTTAGATACAATAAAGATACTCCTAAAAATAATATTAAAGACTTGGATATAATAATTGTTGCAACCGGCGCAAAAGATCCAATTACATACGACCAAGTAAAACCTGGGGCGGTTATTATTGATGTTGGAATACACAGAGATAGCAATAATAAAATTTCAGGGGATTTAAATTTTAATGAATTTGCTAATGTTGCTTCATTTATAACCCCTGTTCCTGGCGGAGTTGGTCCAATGACTATCTCGGCATTGATCATTAATCTTTTAAAGGCAAGTTCTTTACAAAATCCAATTATTTTACCATATCTTAAATTTTTGAAATCATATTTTAATTAAAAATTTTTTAGCTGATTTTTGAAGGCTAAAAATTTTTTTAAATTTGATTTATATTATTATTTTTTTGTAAAATAATATCACATAAAACAGGAGGGAAAAATGATTATAGGTGTTAGTGGTATGATTGCCGCCGGTAAAAGTTCGTTAGCTGAAAAACTTCACCGTTATTTTGAAGGTTCGCTTATGCTTCATGAATTTGAAGAAGATGATGAGGTATTTAATACATTTTTAAAATGACTTTATGAAGGTAAACCTAATTTAACAATAGGATTTCAATCATATATAGTTGAAAATCATTCTACAAAATTTAATAAAATTAAAGAAGAGTTTGAAAAAAGCAAAAAAGATCCAATTGAAGATATGATTTTTTTGGATAGATTTTCAATAGAACATTATATTTTTGCAAAATTAATTTTATCTAAAAAAGGTAAAAGATATTTAGATGCATATGATGCTTTGTTTGAAAAATTAATTAGTAAAGATGAATTGCCTGATTTTGCAATCTTTTTAGATATTAATTTTGAAACATTTAAAAAGAGAATATTTACACGCGGAAGACAAAGTGAAGTAGATAACTGAGATGAAAACTACGAATACTTCCGTAATTTACATGAAAACTATTTTAAAATTTTTAAAGAAATTGCTCAAAAATTTGGCTTAAAATATGTTGTAATTGACACAAATAACTTAACAGAAAAAGAAGTTTTTAACAAAGCTTTGGACATTATTGAAAGTGAAAAAGAAAAATGTCGCAAATAGACTATAGAATAAAAAAAATTCTTTTTGATCAAACAACATTAGAAAAGCGTATTAAGGAATTAGCAAATTGAGTCAATGAAACATATAAAGATTCAAAAAATTTAATCATAGTTGGTTTATTAAAGGGCTGTTTGCCATTTTTGGCGCAGTTAATTAAAGACGTAACTGTAGATTTTGTTTTGGATTGCATGATAACTAGCTCATATGATGGAAAAGATAGATCTTCGGGAAATGTCAAAATAACATTGGATTTACAAAATAATATTGCAGATAAAGATGTATTAATTGTTGAAGATATAGTAGATAGTGCTAGAACTATGACCAAAGTTGTTTCACTGCTAAAATCAAGAAATCCTAAATCATTAAAAGTTCTTACTTTACTTGATAAACCATCTGGCAGATTAGTAGAATTTAAACAAGATAACTTTGGTTTTGAAATCGGCAAAGATGACTTTGTTGTTGGCTTTGGCTTCGATTGAGAAGAAAAAATGCGTCAATTACCATACATTGGTTTATTGACAGATAAAGAAATTAATAAACACAAAAAACACTAATAATTTTTCAATGATTCTTAAGTTACAAAATTGGCAAATAACAGAAACTTCAAATCAAGATAGTGGCACTAGCATACCTTTAGCTATTGGCTCTGCGTTTGTGGCATTTATTTTAATAGTTGCTGTAGTTATTTTTATAATAAAAAAAATAATAAATAAGCAATCAAAAACAGAAGATAATTCAACTAATAAAGAATTAAATAAAGACAATAAAAATGACGAATATAATAAAAAAATTACATTGCCTTTTTTTAATTCTGAAGACCTAAAAAGAATAGATAAATTAAATTCCTTAAAAATTGAAAAACAAATTGCTTATTTATTTGACTCGCAAAAATTTTTTAAAGAAAATTTAAATAATTTATGAGTTAAAAAATATTACGAAATTATTAAAAGATTTAAATTAGAAAAATCGCCTGTAAGTAAATTTAATAAATTAAAAATTAATAAATATTACAAAAATTATCTTAATGAAGTGGGCCAATGAAATTATTGCAGACACCACATTGATGAAATAAAAATAAGTGGAGCAATTTATAGTGCTCATCCTCAAACAAAAGAATATTATAAAACTGGTGATGTTATACTGGTAACATATGAACAACACTTATTTTTACATTATTTAATTGTAATGGCAAAAACTACAATGCCAAATCACGGAATGATAGTGGGACTTAAATTTCATTTTAATGATGCTAAGAAATGCATTGAATATTGAGATAAAGTTATTCAAAAAATGTGCGAAAAATATTTAGTAAAATATGACAAAAATTGAAGTGAAAATTTAACTTAATTTAAATAGTGAGTATTCACTATTTTTTATTTTAAAAAAACTAATTTTCTAGATTTATTATAATTATTAATATAAAACATCAAAAAATATTAATTGCAATTTTAATTAATAAGAATTTTTAATTTATTGATAATTTTTAAGGAAAAACATGAAAATAAAGCTTTTTAATAAGATAATAAATGATGAAGATAAACCTAAAAAAACTCATTTAAGTTGAACAGAAAAATTTAAACTTTCGTTGCAAAAAGAAAAAGACAATTTTAAAAATTCAATTCAAAAAACAAAAGACTTTTTTTGCAATCCTAAAAAGTTTTTTAAAGATTATAAAGAAAATGTTTTGAAACCAGAATGAAAAGGATTTGTAGAAAGTTTTAAATTTGAAAATTGACCAATTGTTCAAGCAATAAAAAGACTAAAAAGCCCTAAAGATAACATTAAAAGTAAAGAAGAAAAAATTAAAATAGCAAGATGAAATTTTACTGAAAATAATGACGAAGATCAAACAAATGATAAATAAAATTTGCTAAAATTTTAAATTTTTTAGGTTTTAAAAAATATTTTATTGCCTAGGTTTTTTATAGCTAATTACCATATTTATAAATGTTGCATAAAATATTAAAAAAATTATTTAAAATTAATTTTTTAATAGTGATAAAATCACTATTTTTTATATTTTAAATAAAATATATTAACCTTAATTTATTATAATTATTAACATGATTGAAATTAAAAAAATATCTTATAAATATCCAGGTTCATCGAAGCTAGCGTTAGAAAATATTGATCTTAAAATTGAAGAAGGCCAATATATTGCTATTTTAGGTCATAACGGTTCGGGAAAAAGCACATTTTCTAAACTTTTATCTGCAATTTATAAACCAACATCAGGAAAAATAATAATTGATGGATTAGAAATTAATTCAGAAAATTTACTAGAAATTAGAAAAAAAATTGGAATCGTATTTCAAAATCCTGATAACCAATTTATTGGTTCAACTGTTGAAGATGATATTGCTTTTGGTCTTGAAAATAAAATGTTGAAACCTGAAGAAATGAAAACAATTATTTATAAATATGCTCAAGAAGTCGGAATGCTAGATCAGTTAGATAGAGAACCACAAAATCTATCTGGTGGTCAAAAACAACGCGTTGCAATTGCTTCAACATTAGCCCTAGATCCTAAAATAATTATTTTCGATGAAATTACTTCAATGCTAGATCCTAAAGGGCGTAATGATATTTATGAAATAATTCATAATTTACACAAAAAAACTAATAAAACATTAATTTCTATCACACATGATATGGATGAAGCACTACTAGCAGATAAATTAATAGTATTTGCTGGCGGTCATGTTATTGCTCAAGGTAAACCAATAGAAATCCTAAATAACAAAGAAATTATTGAAATTGCAAAAATAGATTCTCCATTTATCTATAAACTAAGTAGTCTCTTAAATGATATTGAGCCAACATATAATAGAGAGGAATTAATTAAAGAAATATGCAAATAAAAGCTTCTGAAATTACCAAAGAATACGACAAAGGATTGCCCACTTATATAAAAGTTATTGATAATGTAAGTTTAGAAATATCAGAAGGAGAAGCAATATCCATTATTGGCCCAACCGGTTCAGGGAAAACAACTCTTATTGAACATTTTAATGGTTTAATTATTCCTTCAACTGGAAAAATTATTTTTAATGAGTTGCCAATAAAAATTAAAACTCCAAAACCTAAAAAACCTAACTTAAAAAAAATTAAACATGATTCAAATGAATATGAAATTGTTCTTGAAAAATGAAAAAAAGATCTAAAAGATTGAAAATTAAAGAAAAAATCCGAAAGGTTTGAGTGAGTTACAACCGATTTAGAAATTTCTAAAACCATAAGAAAAATAAAAAACATTAAAGCATTAAGAAAACAAGTGGGAGTTGTATTTCAATTTGCTGAATATCAACTTTTTGAATCAACCATTGAAAAAGATATAATTTTTGGACCTATTTCAATGGGTGTAAGTAAAGATGAGGCTAAAAAATTAGCACTTAAATATATTGAAATGGTTGGATTGCCTATTGACTATTTGCAAAGAAGTCCATTTAATTTATCAGGCGGTCAAAAACGTAGAGTTGCGTTAGCTGGTATATTAGCAATGGAACCAAAATTTCTAGTTTTAGATGAACCAACTGCCGGGCTTGATCCGCAAGGTGTCGAAGAAATGCTAAACTTATTTCATAATCTCTATTTAAATGGAAAAACGATTATTATTGTAACACATGACCTTGATAATGCCTTAAAATGAACAAATAGAACAATATTTGTAAAAGACGGAAAAATAATTAGAGACGGAAATACATATGATATTTTGTCAGATGACAATTTCTTAATAGAAAATAATTTAATTCCTACTAGATTATTATCGTTTGTTAATGAATTAAAAAAGAATAATGTAGATGTAGGTAAGGTAACAAGTGTTGAAGATTTAGCTGAAAAAATTAATTATTACTTAACCAAGAAGCGCAAGGGAGGAACAAATGAATAAAACAATTATTGGTAAATATTTAAATTACGATACATTTATTCACAAAATTGATCCTCGCTTCAAATTTTTAACAAATGTTTTATTAATAATATTGCTTTTTATGGCTGATCACTTTATAACTTTGGGAATTTTAATTTTCTCAATAATGATTTTTTATTGAGTAGCCACAAAATCATTAAAATCACTATTTTCTAAGTTGCGCTTGCCACTATATATTGCAATATTTCTATTGATAATAAATATGTTTACAGTTAAAGGAGCAGTCTCTTTAAATTCGGTTTTAAGTTATGTTCCACTTGCTTCTGATGGAAATTTAGACTCCTATGTCTATTATAGAAATGAAACAATAATTAATACAATTTATTGAGCCCCATTTGGAAGTGAAAATATATTTCAATTAACCAAATTTACAATTTATAGAACGTTATCAATATTTTTAAGAATATATGGTGTTATTTTAACAACAACAATTTTAACAGTTTCAACAAAACCAGTTTTACTAACTAGAGCAATTAATGATATTTTAATCCCTTTAAAGTTAGTTAAAATTCCTACAGAAATTATAACAATGATTATTTCAATATCATTACGTTTCATCCCAACATTGCTTGAAGAATCCAGTCGTATCATGAAAGCACAAGCAAGTAGAGGTATCGATTTTAAAAACGGAAATTTAAAGGATAAGACTAAATCCTTTATTGTTTTGATTGTTCCACTATTTGTTTCTTCATTTTCAAAGGCGAATGATTTGGCTGACGCAATGACATCAAGAGGTTATGAACCTTATGCTAAAAGATCTCACTATAGAGTGTTGACACCTTGTTGAAGAGATATAATTGCTGTATTAGTCATAGCTAGTTTAACAACATTAGTTTTAGTGGCTCAACTTGATGCTGTTCCATTGCCAAAATGATGATTAGTAACATTTCAAAGAGTTTAATATGAATAGCAATAACGATATAGAAAAAATAAGAAAAGAAATTTTTGATTTGCAGGCTAAAATTAGTGCTTGAGATAATGCATATTATAATTTAGATTCGCCTGAAGTTCCAGATGAAATTTATGATGTTGAATTTAATAAATTAAAGGCACTTGAAGAAAAATATGCCTCGTTTTTTAGTGATGAAGAATTAAAAAATTCTCCAACAAGAAAAATTAATGCATTGGCTTCCGATCTATTTAAAAAAGTAAAACATAAACGTCCAATGCTATCTTTAAATAAAGCGTATTCGTTAGATGAAATTCAAAAATTTATTGATAATATAAAGAAAATTACGTCTGATTTTTCTTTTTTTATTGAGCCTAAAATTGATGGTCTTTCAATATCTATTATTTACGAAGATGGCAAATTAGTTAGAGCAATAACAAGAGGAGATGGAATAACTGGCGAAGATGTTACGGCAAATGTTATTCAAATGTCTTGCATTCCTAAAACTATTAAATATAAGAAATCATTAGAGGTAAGAGGGGAAGTTTATTTAGCAATATCTGATTTTGAAAAATTAAATTTAAAATTAGGACAAGAAAATAAAAATAAACTTGCAAATCCTAGAAATGCAGCAGCAGGCACATTAAGGCAATTAAATTCTCAAATTATTAAAGAACGCAATTTGTCAGCATTTTTGTATTATGTTGTGGACCCTTTGCAACATAATATTAAAACAATGCAAGAAGAACATGAGTTTTTAAGAACTTATGGTTTTCCTGTTACTCCTGAAGTTCAACACGCTAACACATTACAAGATATTGAAGAATATATTAGAAACTTCAAGAATCAGAAACTATTAATAGATTACGAAACAGATGGAGTTGTTATAAAGTTGAACGAAGTTCAATATTTTGACAAGTTAGGATCAACGGCTAAGTTTCCCCATTATGCAATTGCATTTAAATATGAACCTAATGTTGAAACAACAACAATAAAAAGAATATACGCAACAGTAGGAAGAACAGGATTAGTTACGTATAATGCAGAATTAGAACCTGTAGAAATTTCTGGTAGTCTCGTAAGCTTTGCAACATTGAATAATTTTCAATATATAAAAGATTTAAATCTAAATGAAAATGATTTAGTATATATTAAAAAAGCTGGAGAGATTATTCCTTGTGTAATTGGCTTAATTTCTGGCAAAAAAGAAATTGATCATTTTCAAAAAGTAGAATTCTGCCCATATTGTCACAATAAACTTGTCGATTCAGAAACGGGATTGGAGCAATTTTGCTCAAATGATCAATGTCCTGAAATACAAAGAAGAAAATTGATTCATTTTTGTTCAAAAGACGCTTTAGATATTAATACTATGGGTGAGAGAAATATTGATCTATTTTTAAAAAATTCTTTAATTTCCAACCCTATTGATATTTTTAATTTAAAGAACAAAAAACAAGATTTATTAAAATTAAATAGAATGGGTGAAAAATCCATTGAAAATATTTTGATTTCCATTGATGAATGTAAAAATAAACCTTTATATAAAATGATTTCTGCCCTTTCAATCCCTCTAATTGGTGTAAAATTAGCTAAATTCGTATCCTCGAAAATTAAATCGTTACATAATTTATTAAACTATGACTTTTCGCAATTAATTGATTATCATGAAATAGGAGATAAGATTATAAATTCTATAATTTCTTGAATATCTAATGATTCTAATAAAAAGTTAGTTGAGGACATTATTAATCTTGGATATGATTTAAAAGATGAAAATGAATCAATTACTAATTTATTGGAAAATAAATCATTTGTAATAACAGGAACATTGGGACATCCTAGATCATATTTCGAAAGGTTAATTGAACAAAATGGTGGTTTAATTCACTCAAGTATTTCATCTAAAACTAATTATTTATTAGCAGGTGAAAATGCTGGCTCTAAATTGCAAAAGGCAAATAAATTAAATATTACTATAATAACTGAAGAAGATTTATTTAAAATGATTGGTCAATAAATAATATTCTCTTTTTATTTTAATATATGTATATATTGAAATAATATAAAAATTAATTGAATATCCTAATATTGAATTTTTTTCTACCTCAATAATCAAACAACCATAGCTTAAAGTATTTATTGCTATTTCAAATATTGAAACATACATTTTTATTATAGGTTTAACTATAAAAGCAAATAAACAAAATACATAGTTAAATATTGAAATTGGTAGAAAGAATAATTGATAAAATATTCCTAAAATGTTGAATTTTGGATTAAATGAAATTACTATTGAAATAGAAGAAATATAAGGTAATATGATAAATAGAAAATTTACAAATATATTGTTTTTGTAAATTTTTTTAATGTCTAAAATTAAAAAAATAATCAATGTTATTGTAAAAGATAAAATAAAAGAATAAGAATACATGGTTCTAATATCAAAGAATATCAATAATAGTGCTGTAAAACTCAATATGTCAATGTTATTAAATTTGCCTTTAAAAATGTTTTTGCTTGCAATTTTTAATATCAAAAACAAAAACGCCCGACTTGCAGATATTGAAAAATTTAATAACCTTAGGTAAAAAAATAACATTAATATTGAAATCAAATCAACAACCAATTCCTTTGAATTCAATCATTTTAAAATTTTTGATATAAGCAAATATATTATTCCAAAATGAAATCCACTTATTGTAAAATAGTGCATAACACTTAGTTGTTTTAATTGATCTACAAACAAATCATTCGCTTGTTGAATGTTGAATAAAACCAGTGCTAGAAATCTTTTTGTTTTATAATCATAATCTTGCAATATTGGAATTTTTTCTTGATATGCATATGCTTTTGAAACCAATTTAACATTTAATAATTCAAAATCAATTTTATTGCTCAAACTAAACATATTTTGATTTTTAATTATTGATAAAGTGCCTGATAATTCAATATTATATTTGTAAAAATTATTGAATTCTCAACTGCTCCAATTTTTAACTAAGACATTATAATATTGATATTTAATTAATATTGATTTATTAAAAAATCTCTTTATTTCAAACTTACCACAAATATTCTTTCCATTTATTTTAAATAAATCCAAATATCACAAATAATGAACTAAAAATATTAAAGGAAATACTATTGCAATTTTTATTCAATATTTTAAATTAAAAAAATTCACTGCAATTATTAATGACAACATTATTGAGGATATAATAATATTAATTTTATAAATAATTAAACAATATAAGCAAAAGCTCAGCGCTACAAATAAATTTGTGTTTAAATTACTAAATGATTTTTTAATATAGTTAAAGTTTTTTCACCTACTCCTGGTAAATTTTCCAATTCCTGTCATGTTATTAAATAATCCTTAGCAATGAAGTAGCTTAAAATAATTTTTGCTATATCTTTTTTTATTCCTAAATCCTCTAATATGCCAACGTTATTTACTTCTCTAATAGAAATTGAATTATCTTTTTTAAAAGGAATATTAATTGATTGATTTTTTTCTAACTCTAAATTTAAATCAATATTGTTTAAATCTGCGCCCGCTTTAAGACGTGCTTTTTTTATTATCTCCAATAATTTAGTGCCTTTTTTAATTTCGTATGAGTCGGGATATTCTATTGCGCCTGATATTCCTATTGTTATTATTTCATTTTTGTTTTTGATAAATAATTTATTGTTATTTTTTATATTTGAAATGTATTTATTAATTCCTATTGACATTAGTATTATTATTGATGCTGATGAAAATAATCCTAGTCCCAAAAAAATTTTTCGTTTATTCATTTATCCCTCCATCTTTATCTTAAATTAAAGGATTTTATTATTGATGAAAATGGGAAAAATTAGCGAAAAAAACCGTTTTTGGTTTTATATAAAAAAATAAAAAACGCAAAGCGTTTTTTATTATTCTCAGATTGCGTTGTGTCAAACTTCTTGGACGTCTTCATCGTCTTCTAAAAAGTCAACAAATTGTTCAATTTGGCTTGCTTTTTCTTCATCTACTTCAATTTCGGTATTTGGGATATATGTAACTTCAGCTGTTGTATAATTTTCAATTTTGAATGTTTCATCTAATGCTTGTTTTACTTTTTGAAATGCTGAAGGGCTTGTAATTATTGTATAAGTTCCATCTTCATTTCTAAAATCTTCTGCTCCCGCATCTAAAACTGTCATCATGGTTTCTTCTTCACCAGAAACATTACTTGGAAATTCAATTAAACCTTTTTGATCAAAAACATATGGTATTATTCCTTGTTTTCCTAATTGACCATTGTGTTTTGAAAAGAAATGTTTGATATTTGAGCTTAAACGATTGAAATTATCTGTTAAACAACTTACAATAAAATTTACACCGCCAAATGCTGTACCTGAATATAAATAAGATTGATAGTTTGCAGCTTCTTTAGATCCTCCAGCTACTTTTGCAATTGCCTTTTCAATGTTTGCTTTAGGCATACTTTTTGCCTTAGCCTTAGCAATTGCTAATTTTAGCGATGGGTTAGAGTCTGGATCTGGTCCGCCTAATGTTGCAGCAACAATAATTTCTTTTGAAAATTTTTGAAACATTTGGCTTCTTTTAGCATCGACTGCAGCCTTGTGATTTTTTGTGGTAGCTCACTTTGAGTGTCCTGACATATTTTCTCCTAAATTTGATAGATAAATATAGGATATTATACCAAAAATAACACTCTTATTTTAGTTATTTTATATAATAAGAATATGTATTTAATAAGAGGAGAAGATAGTTATTTAATAAATCAAGAATTACAAAAAATTATTGATTTAAAATTTGAAAATCAAGACGATTATTCTGTAATTACTTACAATGAATCTATTGATTTAGAGCAACTATCAGATGATATATTTAACCAATCATTATTTTCTTTTAAAAAACAAGTCATAGTTTTAAAAAACATTGATTTTTTTAATTCTAAGAAAAAAAATGACAATAAAAAAATGAACGATTTTTTACAATTGCTTGAAAAAGGGCAAGATAACAATTTAATATTTATTTTGCAAGATATTAAAAAAAATGATATTTCTTTTAATCCTTCATTGGCTTATAAAAAAATTGAATATATTTCAAAAATAATTGAAATTAAAAAACCAAGTGATAAGGATGTATATAACTATATAAAGGAATATATTTTAAAAAATGGAGGCAATATATCTTCAACTACATTACTTGATTTTTTGTCAATGTTTCCTAATGATTTACAACAAATTATTAATGAAGTAGATAAATTGCTATTGCAAAATAAAAATATTGATATGTTGTTGATCAATAATTCAAATATTATTTCTTCTGACAACATTGAATTTAATTTATCAAATTGTATTTTAAAACAAAAAAGCATTGATATTATAGAAGCAGTAGATGAACAAAAGAAATATGGTAATGGTGCTAGTGAAATTCTAAGCCAAATAAGTTATTTGTTTTATCAAATTTATTGCACGTATTTGTTGATGGAAAATACCAGTGATAATGAATATATATCAAAAACTTTATCAATACATTCATATAGAATCAAGTTGTTTATGTCGTATATAAAAAAAACAAACAAGCATAAATTAAAGGAATTAATAGAGTTACTTGCTGATATAGATATAGCAATTAAATCAGGATTATTAGATGAAGAAAATGCCTTAAATAAATTTATTTTATTTTACATAGAAATATAAAGTTGGTGTTATGAAAAAAATTTCTATTTATGAACTTAATTTAAGCACTTTTAATAATAAAAAGGGTTTAAACAAGTATTTGTCAATTATTGAAAAAATTGAATATTTAAAATCATTAAATATTGATATCATTGCAATTGATGATATATTATTGAAATGCTATGAAATCAATAGAAATTATGAATTTAATGATTGATCGAATTTGCAAGATTTTAAAAAATTAGTTGAACTTTGTTCGGCAAATAACATTAAAATAATGCCAACAATCAATATTGCAAAATTAAAAAAAAGTATTATTGCAAAAAAGCATTTAATTTCTATTTATCGAAACAAAAGCAGTGAAAATGAATCAAATAAAAATGATATTGAATCTTATTTATTAACCCAAGAATTTCTAGTTCCCACCATTGAAAATATTTCAAATTTAAGCGTTTTCTTAAAAGAAATTATTAGTTTTTATAGTGTATTAGGCATCAAGGGATTTATTCTTGAAGATTTTGAATTCTTAATCAATAAAAAAACAAATAACATGAAGTCAAAATTTAGTTTTTTATTAGATATATATAGAATTATCAAACACATAAATTTTAGTTCTATGGTTATTTGAAAAACAAAGCAATATAATGATTTTTTAAACATAATTGATATTGCTAATCAAAACGATTTAATATTTTTTGATTATATTTATTTAACTCATTTATCTGACATAAATTTAAAAAATTCATGAGATTGAATTAAATTCAATAAACTAAATGTAAAAAAATTATTTGAATTGTGAAAACCTTTTTGCAATAATGAGCAATATATACTTTCTTTATCATCAAATTATAGCGGCTGAGTTGTAAACAAATACGGAGACAATTTATCATTTTATAAAGAGAGCGCAAAATCTCTATTAATGTTTTTAATGCTTGCAAAAAATTCTTATAGTTTATACAATGGCGATGAATTAGGAATTCTTGGTACTGATTCAAATAATATTTTTAATTTAAATAACATTAATTTTAACGAAGAAAAAAGATTTTATCAATCTAGGAATATTTCTAGTCAAAAATATATTGAGTCTCAAATTAAATTCAATCCAATAACACTACAAATAGAATTTCCATGGACTAATAACATTCAAAATTTAGATTTTATACAGCCAAAATTGCGTAACAAAATTAATGTTGAACAACAGTTAACCAGTAAAGATAGCACGTTTAATTTTTATTCAAATTTAGTTAGAATTTTAAACAAATCAAAATATTCAATTTATTTTAAGGGTTTTCCCAAAGTTTTACTTTGCAATAATTTAATAAGTATTGAATATAGTACTAGTGAAAATAAAAAACTTTTAATTTTATTAAACTTAAATTCGTTTCCAATAAAATCATATATATGAAATCAATATCTAATTCTTTCAAGTTCTTATACAAATAAATACTATTCAAGTATTCCGGAATATTTAAGTCCATATGAATGTTTAATTTTAATTAAAGATAATAATTAATTAGTTTATAATTTTTAATATATTTTATTTATTTAATAATAAAGGAGTCTTATGCAACTACAATTCGATGCATCACAAGTAGCAAAGGAACAAAAGGCGTCAACAGTTGGTTCTGCAATTGTTTATACACTTTTAGCTATAGTTTTGGCTGCCGTCTTAGCTTTTTTAATTTGAAAATTAATTAAAAATAAAGTTTTAAAGAAGAGAGCTCAAAAAGTGGAAATGCAAAAACAAAAAGAAACGTTAGCACTATTTTATCAATATATTTTAAGCTTTTATGAAGTTATAAAATTTACAAACCAAGAACTTAAAAATTTTGAAGTTTCAATAAGCACTCATCCAATGGGTGAAATTAAAGAAGGCGCTAAAAGGTTACTATATCGTTTAATTACAAGAGATGATTTTTCATATTCATTTGTTAAAAATGAACAATACAAAACCTTTGTAAAACACGCCGAATTAATAAATATTACAAATTGCAACTTATGAGATAAAAAAATACCTGAAACAATCAATTATTTTAAAGAACAATATGAATTAGTCCCTTTAGGAAAAACAAGAGATGACTATATACAATTAGTTCAAAAATCAATATCTGAAAGATTTTATAATGAAAAATAAAAGTCAAAAGAAACAAAAGATAATAGTCTCTAAATTTGGTGTTGCAAATTGATTAACAGTTTTAAGAATGTTGCTAATGATTCCTTTTATTGTTTTTCTTTCAACTTCATTTGGTTTAATTTTAAATGGTGTTCAACTTTCTTACAAAACCAACAAAGGAATAGTATTATCCATTTTATATTGAATAAATGTTGCAATTTTTATCATTGCAATGATAACTGATTTCTTAGATGGATTTTATGCTAGAAAAACAAAGACCGTATCTTCATTTGGAAAGATATTTGATCCAATTGCTGATAAAATTGCAACTACATTGATGTTATTATTCTTAGCGACATATAATTTTGTTTATATACCTATTGCAATTTTGTTTATTATAAGAGACATAATTGTTGATGGTACTAGAGTATATGCTGTAAAGAAAAACATTGAAGTATCAGCAAATTGATTTGGCAAAATTAAAACCATTTTAGTTTCATTAGCGCTAGTAGTGATTTCAATAGGAGTTCCTTTTATATCAGATAAATCAGATTATAAATATATACTATTTTATTTCAATATACCTTTAATAATAGGACTATTATTAGCGTGAATTAGTGGCATTATCTATATTATTAAATATACAAAGGGAATTAATAAAGAATTGCTTCAATCATATACAAAAAATGATTCAGATGCAACAATTGAAAATAATCAAGATCAAAATAATAATAATAATAAATCAAATCAAGATTCTAGCCCATCGCCTAATTGCTAATTTCAAATAAAATTTAAAATGTAACTCAATTGTTACATTTTTTATATTCTTTTCAATAAAATCTCTCAATTATTATTGATTAAATTTATAATTTAATCAAAATACGTTTAAAAAATTTATAATTCTAAATATATTTTAAATGTGAGGTACTTGCAATGAAATCAAAGAAAATAAAGTTATTAAAGTTATTATTAACATCGTCAGTTGCAATTTCGTGTGCAATATTACCTGTTTCTTGTTCTTTATTATCTAATGATAATGATAAAAAAATAACAATAAAGAATTATGAATTTAATAATTTATCAAGTGAATACACAATTGAATCTAAAAAATTAAATTTATCATTTGTTAATGATGGCAATGTTCCATATGTTAATTTGCTACAAATGATAAATTCATTAGATGGATTTTTTGATAAAAACTATATTAATTTTTCAACAAATATTCTATTTTCTGAATTGAAAATGGCAACTAAATTTGGATATATTTCGTTTAACTGAAAAACAAATAAAATTACTATGCCAAACACTTATTATGAATATATAACAAAAAGCCCAGGAAAGACTAATTTCAACAAATATTTGAAATCCAAATCATATTATTCAAAGGATTTTAATAAAAATACAATTGTTATAGATTTAAAAAAATATAATTTTGACATTTTATATCATAACGGTAATGTTTTGCTGCCATTAGCAATATTTAATTTGTTATTTTGCTCAACTAATTATTACAATTTGTATTTCAATGGTAACAAAATCTATGGGTGCGATTTTGACATTAAATCTAATGCTAAAGAAATTCCTCAAATTAGAAATAGCAAATTTCAAAACTCACTTATTCCCAAGGATATAGTTGAAGCAAAATTTAACTTCACTGCCTTACTAATTGATTATTTTTACGGCCTTAAAGAATATAATAATATTAATAGTTTTTATTTCACAATTTCAAATGAACAAAAGCAAAGAATGCTTAGTCAAAATCCAGTTATTTCAACAAAGGAATATATAAAATATTTATACAATTTAAATGATTTACACACCTGAGTTTCAATGCCTTCATTTTATAATCACAAGGATTCTAGCATTGCAAAAGATTTCTATGAATATACCACAAATAAAAGAGAAAGAATTTATCAATCTACAATAAAATATCTAAAAAATAATAAATTAAATAATTGAGTAATTCAAACAAGCAAAGAAAACAGAGAATTATTCGAAAAATATGGTGTTAGATTCATTTCGAACAATACCGCAGTAATTTCATTTAACTCATTTAAAACCTTTCAAAATTTAAATGATAATTCTACTAATAAAGGATTTAGTGAAGACACATATTTAGGGTTTGCTAATGCTATGAAATTAATCAAGAAAAACCTAAGAAATATGAAAATAAAAAACATTATTGTAGATTTATCAACCAATGGTGGTGGCGATATGGGTGCTATGCAAAGGGGGTTGGGTTTTATCACAGATGAACCAATATTAAACAAAACATATAATCGTTTAAATGAAGAAATAAATATTAGATCTGTTGAAGTTGATATAGATGGCGATGGAAATTACAAAAATGATGCTTATTCTGAATACAATTGATATCTTTTAACCGGAGCAAACACTTTTAGTGCTGCAAATACCTTTACTTCAATTTTTAAAGAAATGAAATTAGGCAAGATAATAGGACAAAAAACAGGCGGCGGTGCAGCATCAATTTTACCTGTTGTGTTGCCAGATGGAACTTCAATAGTAATAAGTTCTAATAATTTATCTGTAACTATGAAAAATAATACAATTAAATCTATTGAAGGTGGAATAGAACCTGATATTTACTTAGAATACAAAGATTTTTATAATGATCAAAAACTATTAGAAATAATAAAAAATAAATAAATCAACACTTATCATCATTTTTAAGTAGATTGAAATTCATATTGTTCAATGTTTAAAAATATTCAACAAAAACAAGATAAGAGTTTTTAAATAATTATCTTGTTTTTTTGTATGAGAATATACAACAAAAAAATAACTAAAATAGAAATGTATATTTCACAGTAAATAAATTTTCAAATTAATCAACCTTGTTGAATTTAAAACATCAAACCTACCTAAAACATTAGGCCAATCACCAAACTTTAATTTTTTCTAATTTCTTAAAAAATTAAAAATAGATTTTTGCAAGCTATTGCAATTTAAAAATGTTTTTTGGCTACAACGCATTTTTTGTAATATAAAAATGAATTACATCAATATAAACAATTAGATAATAATTAAAATAAAAAACCTTTCATAGTAATTTTATCTACTAAGAAAGGTTTTTATAATTACTAATTTTTAATAATTAATGTTAACAATAGTTTTTATTTAGCGCTTTTTTTAGCATTATTTATTGCTTTAAAAACTTTTGGCAGTTGAGATGTTAAAACATTTTTTAAATCTTTACTATAAATATTTGTGATTAGTTGCTTAAATATCAATATTATTCCTTTAAATTCATTAAAAATAATTTCATATTCTTGTTTTGTTATTTTATTTGAATTTACCATATCATTTAATTTCTTTTTTAAATCTTTGCCGGATGGATCAGAATATTTTGGCTCTATAAATTTTTCTACATCAAATAATATAGGTCATCCACCAAATAATATCAATACTTTCTTTCCTCCACCTTTATTATCATATTCTGTTTGTTTTGGACCTTCATTGTGGAATAAATAATCAAATTTAAGATTGCAAAAGTAATCCAAAATATATTCTAAATGTTTTATTAACAAGCTTTTTAATTCATTATATTTGTTATCATTTGTTTTTAAAAATTCATGTTTTTCTTTAAAGCTATTCAATATTGGAGTTAAATATTTAATAAAAACATCAGTTCCATTTTTAAATTCTTTGAACATTTTTATAGCTCAGGCTCCAACTGTCTTTGGTAAAACTCAGTCTGCTGTCAGATTTTTATAATACATAAAACCCCTACCATTAGAAACTTTATCATCAATGCCTAATTCTAATAATACATTTAACATTTCATCATTGGTGTTAATAATTAAATTAATTAAGATGTTGTAAAGTTTTTTAATAGATTCTTTGTTAGAATTAAATTGACCAAATACATTAATTGCTTTATTTGCTAGTTCAAATAACGTTTTTTCTATTTCTTCGTTAAACTTTTTATATTGTTCAATTATTGCTTTAATTACGTTTGCATGAGCGGCTTTTTTGTGTTGTTTTTTTCTTGCTTCATTAATCTTTTTTTCAATATTTGTTTCAATATTTAATTTAACATTTTCTAGATATTTTTTAAATTCATTTGATTTTTTTACATCCGATTCTAAATTTTTAGTTATTTCGCTTACATCCTTAGCAAATCCATAAAATTCAAATGTTCTTGATTTAGTAGATTCTATTGGATTTTGACTATATTCATTCAATAAACTTTTATTTGACTTTAACTTAATTTTATAAGTTAATTTTCCCAATGTTTTATCATATTTAACCGCCTCTATTTCATAGATAGCTGCAGAATCACTATCTTTTAGATTAAATCTATAATCTTGAATTGTAATATCTTTAACATCAATAGATCGCTTTTCAAAATCATTATTTTTAGTGAATTCCATTGTAAGTTTATCCAATAAATCGTCAAGCCTTTTTGGTTCATTTTTTAATTCTTCTTTTATTTTTTCAATGCTTGCTTTGTCATTGAAACCTGATTTAGTAAAGGTTTTTATTTCACTATAATCGTCTAATTTGAATGTTATTTGTATATTTATATCATTTGCATTTTCACTTGCCAGTAGTTTATAATCTTTTTGAATTTTAAGATCATCAATAATATAAGATTCTTCTCCGCTACCAAAATTAATTTTTTCTCTAAAAAAGAAATTGTAGCCTTTTTCTATTGATTCATCTATTGTCATTGATTTTGCTAATTCTTTTTTGGTGTCATCTTTAAAATCAAAATCAAAGTTATTTGCAAAATTTTTTAAATTTCGTTTATTCATAATATTAGCAATAGAAATTTCACCCGAGAATTTTTTAAAAAGTTCATCATTTGTTGATTTTATCTTTAAAATAAATTCATATTTTACAGTAGTATTTGGTCATTCTTTTAATGACACAATAGCGTTTGTATTTTCTTGATTCAATTCGATAATTGAGTTTTGATTTAGAAAATCAGAAATACCTAACAACTTAGCAATTTCTTGTTTATTATTAGCAATTTTATTTTTAAAATCCGCTTTAGTATAAACGCCATCAGTTACATAAGTTGATTCTTTTGAAATGCTTAAGTTATTAATTAAATTAAATGTGTAATCTTCATCACTTAAGAAATTGAATGTAACTGTCTTTTTGTTTGATTCTATTGTTCCATGTTTAAATGTTTTATTCAATAATGAAAAAGTTATTTCTAATTTTCCATTGGCATTGGCGTTGATTTCTGAAATGCTTAATTCCAAGCCTGCTAGTTCATTACTTTTTTGCTTGTTTAATTCAAAGTCATTTTTTGAAATCATTGAAGATTTTTTAGATGTTGAATATTCATTATTTTTTAAAGATATAATATTTTCTCGTTCAACTAATGCATCCAATCTTTTTTGTTCAATATTGATTTCATTTTGCTTTTTAGTTAAGAAATTCGTAATTTTTTTTGAAATTCTTGATTCAGAAACAATAAAGGTTTTAAATTTTTCTTCTTTAACTTTTATTGTTCAACTTATTGACAAAATTCCTTGATCATCATCTGCGATAATTGATGGATTTCCTTCAATAATATATTTTGAATCGCTTATATTTAGATGAATTTCTTTACCTTCAACATTTGAAGGCAATATATTTGACTTATCATTTTCTTTATCAAAAACTACATTTGCTTCTATCTTATCATCGCTAATTAATTTATTCAACTCTTGCTTACGAGCTTTTAAGTATTGTTCTTTTTTATAAAAACCTGTCAATATTATAGTTTGCTCATTTGATTTTAAATCATTATATTGCTTTTCGTTTGTTTTAACAACAAATTTAATTTTTAAGTTTCCTTTATCATCATTTGATTCACTTATTTCTAAAATTTCAATATAAAAATCATTACTAATTTTTGTTTCAAAATCGTTTATTTGAACATCAGAAGCAAATGTTTTGGCAATATTTTTTGAAGAAATTAATCTTAACGTTGTTGTTTCTTTGGCAACTTTATTTAAAGATTCTATTTTTTCGTTTTTATCCATTGTTTTAGTTTTGTAACCACTTAATTCAACTTCAATAAGTTTGCTTGATTCACAATATTCATTAATTTGTTTGTTTAACTGCTTGATTGCTAATCTTATACTTAATTTTCCACTTTCATCGTTTGAAATTAATTGCTTTACAACAACTTCTATATTCTTAAAATTATTATTTGGCTTTGAAATTTCAATATTTAAATCTGATTGCTTGATTGAAGATGCAAATCTTTTAATAGTAGTTTCAGGTTTCTTATCGCTTACTTTTATATTTAATTTTCCTATTTGGTCATTTAAATAAGCAATTGTTTCTTTTCTTTGGTTATCAATAAATTGTGCATTTGTTAAAAAATCTGTCAATTTTACTGTTTTGATTTCTGTTTTTAAATCGCTGAATTTTGGATTAGAACTTTTTAAATAAAAACTAATTAACAATGTTCCTTCTTCGTCATTGGCTTGCATTTTTTCAATAACTAACATTGCTCCATCAATATTTTTTCTATTGAAATCAACATTTTTGATTTCTGAAGGTTTTTTGTTATGCTTTTCAAGTGCCAAAAATTCGTTTAGATCAATTGAATTAACTAAATTAGAAAGTTTCTTTGATGTTTCTTGTCTTTCTATGTTGAGTAATGTATTAAATCCCACAAATTTTTTAGTAACAATATTTGATTTTAGATTCTTTTTATTGTCATTGTATCAATAGCTAATAGTTAATTCACCTTTTTGATCGTCATAACTAAATTTTTTTTGCAATTCATATTCATTATTTAGATTATCGAATTTGAAATTGCTTAAATTTTGAATATATTGAGATGGCAATATTGTATTTATATTTTTTTCATTTATTGATAAATTAACTTTATCAACCGCGATATTAAGATTTTTTAAAAATGATTTAAAGCCCGTAATTTTAGTTGTATAAACCTTTGATTTAATTCAATACGAAGCACCAGTTTTCTTATCAATATATTGTTTTTCAAAATAATAATTTAATTTAACTTCCCCATTTATATCATCAAAAATTAATTCACCATTGCTTCGTAAATAAGTTGAATTTAGTTTTTTAGACGTTTCAAAATCACTTCAATTCAATGTTGAAGCATATGTTTCTGATTTATTAATTTGTTTCCTTAATTTTAAAGATATTTCATTTCCTAACTTATTTAAAGTGTTAGCTTCTTTTTGCATATTAGCTATATTATTCAATGAATTAAAACCATAAAACGTTTTTGAAATAATTTTTGATTTTATATCTTTATCTTTTTGTACATAATAATATTTAACTTCTATTTTGCCTTGTTCATCATTTGCAATATATTCAGTTTTTAATTCATATTGGGAATCGATGTTTTTAAATGAAAAATTGCTTTGCAATTTAATATGTTGCGAAGGCAAAAAGTCTTTTTTATTAAATTCATTTATTTCTAGATTAACTTGTTCAATTGACGAATTTAAAGGAGCAACCAATGCTTTAAATCCTGTATATTTTTGAGAAAACGTATTCGATTTTATTAACAGTGGTTGTCTTGTTTTAGAATCGATTATTGATTTTGTAAAATAATAATTTATAATAACATTCCCGTTTACATCATCTGCAATAATATTAGAGATTTCAAACGATATTTCTTTATCAAGATTATTAACATAAAAATTTGATGAATTAATTTCACTTGGAAGTATGTTATCTTGTTTAATAGAAAGTTTTAAATTTGGTTTAATATTTCTTGTTTGTTCTTTTAATTTTGTTGTTTCAGTTTCTTTATCTTGCGTCTTGTTGCAGGCTGATGAAATGACTCCTGATACAAGTAAAATGGCAGTAGCACTTAAAATGGAAATGCTTTTGATTTTTGTATTCTTTTTCATATCTATTTTTAATATTTCTTTCCTTTTTAATTAGATAAAAAAAGCATAAATGGAAGACTCCCATTTATGCTAAAAGTAAAAATTATTTTAAGATTTCTAACACCTTTGGTAAATCTCTCAATGATTGTTCAAATAAGTTAATTGAGTTTTTTAGACCTTCATTTAAAACGTTGTAAATTGGTCTAATTACGTCTTTTACTTTTTTAATATCTTCATCAGACATTTTTACAGATTTTAATACCGCAACTGCTTCTGCCTTAGCGTCAGATAATAATGTAATTAATTTTTCAAATGTTTTTACATATCCTTCTTCAGCCAATTTGCTAAAATCAAATTCTTTTAATTTCTTAGCGGTTGTTTCAAATAAATTATCTTTTCCAGCAAATTTATCGTCAACAGCTTTAATTAAATCGTTTAATTGAGCTATTGATTTTGTTGATTCATTTGTTTTTAGTGTTTCAAAAATTGCTTTTGCATATTTAGAAATATTTTTTCCAAATGCAACCAAGAAATCTGCCAATCCGTTTTCTTTTTTAATTAAATCAGAAATTTTTTCTTTTGTTAAATAATTTTTAATTGTCATTAATCCATCAGAAACTTTTGTTTTATTTGTGTTGTATTCTTTTAATGTTGACAAAATAATATCATCTAAAATTTCATTTAATTTAGAAATTGCTTCATTATATGTTTTTGTAATATTTGGCAATTCTTTTTCAGAAACTGCAGCTTTAATTTCTTTATTTTCAATAATTGGCTTTAAAATGGCTTCTGATTTAGTTTTTAATTCTTCACGATATTTCTTTAATTCACTTTCAAAATTTGCTTTAACTGTAGCATCTGCAATACCTTCAGTTGCTTTTTGAAGTTCGCTTTTTTCTTCTGGTTTAACTTCTGGTGTTGTTCCTGGTTTTTCACCTTCAGGCTTCTTTTCGGGTGTTGTTCCTGGTTTTTCACCTTCAGGATTCTTTTCTGGTGTTGTTCCTGGATTTCCACCTTCTGGGTTCTTTTGATCTTCTGGTTTAGGTTCAGGTTCTGTTTTTACGCAACCTGCAGCAACCAATGGAGTTGAGGCTAATAATGGAAGTGCTACCCCCATAGTTAATAAAATTTTTTTGGATTTTTTCATTTTATAATTTCCTTTCTATATAAAAATAAAATATATTAACTTCATATAATTTACCATATAGATTGTTTAAGAACAATTTAATAAATTGGATTTCTGAAAAAAAAAAAAAAAATGAACCAAAAAGTAATTTTTTATTGGTTAATTATTAAATTACTTTTTAATTTAAGGTTCTTTTTACAAATAAGAAAAAAATTTTATTATTTCTAAATTCAATGAATAGCTATGCTTTAAAGATTTGTAATATTTAAAATATTAAAAAATGTCTATATAAAAATTAATTTTTTTTAATTTAACTTATAATTTATATTAATTATTTATATATAAATATTTTAAAACACTATATTTTATAGGAGGACACTATGCCAAGAGAAGGTTTAACCTTAAGATGTGAAAAATGTAAAATGGAAAATTACATTACAAAAAAGAACAAAAAATTACATCCAGACAAAATGGAAGTACAAAAATACTGCTCAAAATGTAATGCACATACAACACACAAAGAAAAGAAATAAATTTCTTTATTAGCATTAAAGCTATTAATATAAATAAGGATTTAACATGATTAGAGAAAAATTAAACAAAATTTTAAAAGATAACGGGATTGATGCGATTATTTCAGAAGCTCCTCAAACTCGTTTGTGATATGCCGGAGTTCAAACAAGCGATGGATTTCTTGTTATTGAAAAGGAAAAGGCATATTTATTTGTTGATGGAAGATATATTGAATATGTAACCAAGAATTCAAAAAATGTAGAAGTTGTATTATTAAAAGGCACTTCATTTAAAGATTTTTTGGCTAAAAAGAAATATGCAACTGTTGGTGTTGAAAAGGACTATTTAATACTTGAAACATTTGAAAATTTAAAATCAATGTTGCCAAAATCTAAATTTGTGTTAGTAAATGGACAAGAATTTAGAATACTAAAAGATGAAGAAGAATATAGTAATGTTCAAGAAGCATGTAACATTTCTCTTCAATCTTTTGAACAAATAAAAACCGTTCTAACTGAAGGAATTACAGAATTAGAAGTTTCAAATAAATTAGGCTACTTAATGCGTTTATTTGGAGCTGAAAAAGAATCATTTGAAACAATTGTAGCTTTTGGTACAAATGCTGCTGAACCTCACCACCATCCAACAGATCGTAAACTTGCTGATGGTGATATTGTTAAGATAGACTTTGGTGCTCAATATCAAGGTTGAGCAAGTGATATCACTAGAACATTTTTCTTTGGAAAACCAAAGAATCCAAAACTTGTTGAAATCCTAGATATTGTTAAAGAAGCTCAAAAATTAGGAAGAGAAGCCGTAAAGCCAGGTATTGCTACAATAGAAATTGACAAAATTTGTAGAGATTATATTGAAAGCAAAGGATATGGTCAATACTTCACACACTCAACAGGACATGGAGTTGGAATCGATGTTCATGAACTACCTGGAGTAGGAAGAAACCGTGGAGATGCAATATTAGAAGAAGGTATGATTATTACTGTTGAACCTGGTATATATATTGAAAACGAAGGTGGCGCTAGAATAGAAGACACTATTTTGGTTACAAAAGACGGCTCAAGAGTATTAAGCAGACCCGAAGATTATGAAAAATAATTAAATAAAAAAATGAACAGCTGGAAAACCCAGCTGTTTTTTTCGCTATATTTTCTTTATATTTAAATATTTCTAATTATCAATGATAAAGTTAAATCAAGGAGAATAAATTATATGAACAAAGAAGAATATTTAGCTTATTTAAGTTATATAAATAATGGCAAATGACACCAAATCTATAATGATTTAAAATCACAAATAACATGTCAAAAAACAAAATATACTGATTTTTGTAGTGACCAAGCAATCAAAAACAAATCATACATAACAATTTTTGATAGTGATTATCCGCTATATTGCAATAATGTTTGAAATCCACCGTTTGTCCTTTATTATTATGGAAATAAACAACTATTAAAGCATAAAATTATTATGATAACTGGAAATATTTCTATTTCTAATATTATTGAGGTTAAAGATTCTATTGAAAATTTTAGCAAAGAATATGTCTTCTGCAACGAAGCATGAAAAGGTTTAGACATACAATTAATTGACTATTTAATTTCAAAAAATAAAAATATTATTATTATTTTAGCATGCGGAATTGAATACGCTAAAAATTCTTTGCCTAAGAAATGATTCGATTATAGCAATTTGCTATTTATTTCTGAATACCCTAGCAATTATCACACAAGTAAAATAACGCTAAAAGCGCGAAATAGAATTAGCGCAGCAATTTCTAATTATTTAGTCTTACTCTCTTGCTATAAAAGCACCATGAACAACTTAATAGATCAATTCTTAAATATTGGCAAAGAGGTTTATTGCCTTGATGATATTCAAAATAATTTGAATGAAGATAATTTCAACAAAAACTTAATTAATGAAGGAGCAGGAAAAATAACTTCAATAACTGATTTAATATTAAAATAGAATATTATCTTTTTTATTATCCTCTAAAAGCATTATTATTTCATCTTTGTAAGGACTTTTGTTATTAATAAACGGTGTCTCTAGAATAATAGGAATATTATCGAAGTCTTTATCATGAACAAATTTTTTCAATATTTCTAAACCTATATGGCCTTTATCAATGTTTGCATGACGATCTTTTTTTGATCCAAGTTCATTCATGGAATCATTTAAATGAATAACTCTAATTAAAGGCAATATATTTTGTTGTTTTATATAAGATTTGAAATTTTCATAATCTTTTATATCATACCCCGCATCTCAGGCATGGCAAGTATCAAGACATATTGCAATTCTTTCGGAATTAACGGATTTTATCACATATAATAAATCATTGAAATTGCTACAAATTTGACTGCCTTTACCCGACATTATTTCAAGGCATATTATTACGTCCTTAGTAGATTCAATTATCTTTTTAAGAGATTGAATTAATGATGCTAATGCTTCGCTTCTGCTATATTCAACTGATGAACCTGGGTGTAAAACTAACAATTTAGCACCAAAACTATTCATTCTTTTAATTTCACTACATAAAAATTTAATCCCAAAATCTACCTTTTCTCTATTGGCTAAATTTACTATATATGGGGCATGAACCACAATATCAGATACAGGAATAATACTATTGCATTGCTTTAAATATTTTTCAATATTATATAATGAACTATCAACTCTAAAAGCACTTTGTGGTGCTCCTAAATAAATCATAAAACAATTTTCGCCATTATTAATAGCTTCATTAAATGAGCCTATTAAATAATCAGGTTTTTTAAACGATATATGGCTTCCTAATTTAATCATAAACACTCCTTAAATTTATAAAATTGCCTAATCTATTAATATTTAATTATATTAAATTATAAAGTTTAAATGCTCTTTTTAAATCATTCATAAATTTAGAAATAAAAAATAAGGCTACACGTAATTTTTGATTTATAAATAGCCTTATTAATTGTATATGTTTGCTTTAATCTAAATCATAGCTATCGCTATTAAAGAATTCCTTCAATTTTCTACGTTTTTGAGGATGCTTTAATTTACGCAAAACTTTTGTTTCTAATTGTCTAACTTTTTCTTTTGAAATGCCTAATTCTTTTGAAAGTTCATCTAAAGTATGAGGTTTGTATTTGTTTCCCTTTAAGTCGTTAATTCCAAAACGTTTTCTAATTAAATCCCGATCAGCTTGATCACTTAACCCTTCTTCAATCATTTCATTTATAATAACGCTTAACTCTTCATGGCTTGCATACGAAGTTGGGCTGATTACGCTTTCATCTTTAACAAAATCTGAAAAACTAGAATTTTCTTCTTTTCCAATGTTTTTATCCAAACTAATTGGATCGATATTAATTTTTCTAATATATCTTACTTTTTCAGCAGTAAAATCTCCACCATATTTTTTAGCAATTTCTTCATCTGTTGGAGGATATCCATTTTCTTGTTGCAATTCACGTTCAATCTTCAATATTTTATTTATTGTTTCAACCATATGAACTGGCACTCTCACAGTTCTGGCTTGATCGGCTACTGCCCGTGTTATTGCTTGTCTAATTCATCAAGTAGCATATGTTGAAAATTTAAATCCTTTTGTGTAATCATATTTTGAAACTGCCTTCATTATTCCAGCATTTCCTTCTGATATCAAATCAATAAAATTCAATCCACGATTTTTATAACGCTTTGCATTATTGATTACTAAACGGAGATTTCTTTTGACTAACATATCTCTAGCTTTTTTATATTTATAATAATTATTTGCTTCTTTAGCTTCTTGCATTTTTTTTGCTAGCGCTTGTTCTTCATTTGTAGTTAGTAACTTACCATATTTACCAATTCATCTCATATATCATTTAACAATGTCATTGGTTTCGGTTAGTTTATTAGTTAATTTTATATCATCTTGATCATAAACTTTTTCTTCACTCAACTTTATATCTTCATCATTGTATTCTTTTAGCAAATTGTTAGCATCAAAGTCATCGTATGATTGTTTTTTACCTTGCTTGTGGTTTGTTTGATAATCGTCATCTTCATATTCGTCAAAATCTTCGCCGTAATAATCATTATATTCTTCATCATCAAGATCATTAAATGCCTTTTTAACAAAATGATTATCATCTTCGTCAAAAGACATTTCTGAGTCATCTTCTTCCTTGCTTCTAATTGAACTATCGTCAAATTCTTGATCAATATTTATATCTTCGTCAAGATTTTCTTCTTCCAACTCTTTAATTTTTGAAGGATCAAAATCTTTTTCTTCCTCTTCTGCGTCATCGTCATCATTATCATCATCTTTAGTTGTTGCATGAGCAACTTCGTCATCTAGCGAATTTATGTCAATATCTTCAATATCTTCGTCATCAGTATCAGATGAGACTAGATTAGCTGCAAAAAGGCTTTCAAGTAAAGCTTCTGCTTCTTCGTCATCAACGTAAAGGTTCTTTTTGTTTAGGAATTCAAAAATTTCATCTTGAGTAAAAGCCTTTTTATCCTTATTTTTCTTTTGGACTTTTTTTAACTCGTCTGCAACAATTTTAGTTATACACTTAACTGATGATTCTACATTATTTTTAGTATCTTGCATAATTATTTCCTTCTGTCCAAATTAATTATTAGGTCTTTGGATTGGCCTGTTTCTTTCATTCAATTATTTAAATTTTTATCTAGTTCTTCTTTTGTATATTCTTTGTTTTTAAGACTCATAATTTCCTTGAACAAATCTAAATATATTTGTTTAGCATTATCTTCAAGATCAATATTGTTTGTAATATATGATTCAAAATTTGTTTCATCTATTGATTTATTATTTTTTATGCTGTTTATTAAAAAACACAAAAACATTTTATGATTTTGACATCCATTTGGAAATGCCATTCTAGCAAAATCAACAAGTTGATAGTTAGCAATGTATTGTGGGTGCTTTATAATGGTTTTTATTATTAAATAAAATACTAAATTTCTACCAGTTATTAATTTAGGTTGTTGTATTAAAGGTTCTTCAAAAAAATCAAGTCTATCAAACGAGGGATCAGAATATGCAAAATTAAAATCTTCGTATTTTTTGTAATTTGAGTTTTTAGAATAATTTTTTTTATTATATTCACTATCTAGACTAGCTTTTAAATTATTTTTTGAAATAGGATGAAGTGGGAAGTTTTCTTTACCATATAAGAATAAATATTTGTTTCAAGTTTCAATTGAAAAAATTTTATTTTCAATCAATTTTTCTTTTAATTTCAATATTAATGGTTCTTGCAAATAAATAACAAATTCAAACAATTCAGCATTTAATTCAACCTTTCTTGATTCATTGGTTTCCAATTGAAATACTTGTTTAAATTCATTGAATAAATACATAAATAAATCGACAGGACAACATGCTATCTCAGACAATGTTTTTCCTTCGTCAATATTATATAATTCGTCAGGATCTTTGCTATGTTCATTCAAAACAAAAGAAACTTTTAAATTATATCTTTTTTGATTTGCTAATATTATTCTTAAATTTTTCTTAGTAGCATTTTTGCCGGCAAAATCATTATCAAAAAACAAAACAATATTACAATTTCTTAAAGATTCTAAATTTTGTATAGATAAAGCCGTGCCTAAGAGTGCTACTGCATTTTTTATGTTTATTTTATAAAGAGCAATGCAATCAAATTGCCCCTCAACTAAATAAACACTTGATTTCTTTATAATTTCATCCAATGCATGATAGTAATTAAACATAACTTCATGCTTTTTAAAAACTATTGTTTCAGAACTATTTAAATATTTTGGTTCAACATTATTATAAATACTTCTTCCAGAAAAAGCAACAATTCTTCCATCCTTATTTTTGATTGGAAACATCAATCTATCATTAAAAAAGTTATCAAAATTGTTGCTTGCTATCAATGATGAATTGGCTAAATTAAATGATGAACAATTTTTAGCAATTAGCATATTACATAAATTTTTATCTTTATCAGCAAAGCCAATTTCAAATTCCTTAATAAGTGGTTTTGTTAACCTTCTTTTTTTAAGAAATAATTTTAATTGCTCATTTTGATTAATTTCTAAAATTTCAAATTGAAATATATCATTTGCTATTTGATTTATATTGTATAAATCTTCTTGTTCTTTTGTTGTAGTAGTTATACTATTACTAAACCTTGTTAAATCGATATTTAAATTGAATTTTGAAACAAGCTCCTTGATACTTTCAAGAGGGCTTTTTTTAGTATAAAGTTCAATGAAAGTTATTAAATTTCCGCTTTTAGAGCAACCAAAACACTTAAATATTTGTTTTTCTTGTGACACAACAAAAGAAGGAGTTTTTTCGCCATGAAATGGGCAACAAGCCACATAATTCTTTCCTTTTTTTGTCAAAGAAATATATAAAGAAATAACATCAACAATGTTTAACTTTTGTTTAACAATTTCAAATATATTATCTTCTGCCATTTAGCTCCTTTTATTTTTTATAATTTTCAAAAAACTTAATTAAGTTGTCTAATTCAACTACATGTTGTTTCATAGAATCGCGTTCTCTGACAGTTACTGTATTTTTTTCTAATGTGTCAAAATCTATTGTTACTGCAAATGGTGTACCAATTGCATCTTGACGACGGTAACGTTTTCCTATCGAACCGCTTTCATCATAAATTAATCTTAATTCAGTTTTTTGCAATAAATTATCAAATATTTCTTTAGCCTTAGAGTTAAGTTTTTTCATTAACGGAAAAATCGCAACTTGATATGGTGCAATATTGAAATCAAGTTTTAAAACAATTCTTACATCATTATTTTCTAGTTCTTGTTCTTCATATGAATCTACAAGCAACGCAAGCAATAAACGATCTAAACCAATTGAAGGTTCAATAACATGAGGAATTATTTTAGTTCCATCGTCTAACAAATATTCAAGAGATTCATTTGAATATTTCATATGTTGACTTAAATCATAATTGCCACGATTTGCAATACCAAGCAATTCTCCTCAACCAAATGGAAACAAATATTCAATGTCGCTAGTACCTTTTGAATAATGGCTTAATTCTTCTTTTTCGTGTGGTCTAATTCTTATATTTTCCGACTTTAATCCCAAAAGTGTAACAAAATCAACGCATTTTTTAATTCAATAATGGTAAAACTTTTCATCTTCACTAGGATTGCAGAAATATTCTAATTCCATTTGTTCAAATTCCCTTGTTCTAAAAATAAAATCCCTAGGTGTAATTTCATTTCTAAAACTTTTACCAATTTGGCCAATTCCAAAAGGTAACTTAGCTCTAGTTGTTCTTAAGACATTTTTAAAATTTATAAAAATTCCTTGGGCTGTTTCAGGACGCAAATATGTTTGATTTGCTGCTGTATCAATTGCACCCATTTGGGTTTTAAACATTAAATTAAATGGCCTAATTGAAGATCATTCAGTTTTTGAACCATCATATTCCTTAACTTTTTCTTTAATTATTTCTTCAAGTTCTTCAAATGTTGCTTTTTCAACATTAATTTCTGGAAATAAATCTTTAACAATATGGTCTGCCCTATAACGTTTTTGATTTAGTTTGTTTTCAATTAAAAAATCTGAAAAATTGTCTAAATGCCCACTGGCTTTTCAAACTTTTGGATTCATAATAATTTTTGAATCAATTTGAACATTTCCTTCTTTGGCAATAAATTCTCTTACTCAATATTTCTTAATTGCAGTCATAATATTAACTGCTAATGGCCCATAATCCCAGGTGTTTATTAAGCCTCCATAGATTTCTGAATTTTGATAAACAAAACCAGAAACTTTTAAGTGATTTATTATATTTTGAATATTTTTTTGTATTTTATCTCCCATAAAAACTCCACAATTTTATTTTATATAAAATAACAATGAAATTTTACCATATTTTTCCTTTTTCATTATTCAAAAAATAAAAATGATCATTGCTTTTTTTGAAATTGTTTCAATAATATATTGCTTTGTTAGTATAATAATTACATGTTAGATTTTATTCAAAAAAGAATGCAAAATTCAATACAAAAAATTAATAAAAAAATGTCTATTAATGAAGAAGACATTTTAGAAATTTTAAGAGAAATTAAAATATCGTTATTGGAGGCAGACGTTAACCTTGAAGTTGTTAAAAACTTCACTAAAGAAGTAAAGGCAAAAGCCCTTTCTTCTCAAATTATTGGAAAGTTAAATCAGCAACAAACAGTCCTAAAAATATTCAAAGATGAATTGACTAGAATTTTAGGTTCAAAATCAATTGATATTAAAACAAACAATAACCCTACTAAAATAATGATGGTAGGATTGCAAGGTTCAGGTAAAACTACTACTGCTGCAAAATTGTCTGTTTATTTCAAAAAGAAGAATTTGGCAGTAAACCCTTTGCTTGTTGGTGATGATATTTATAGACCTGCTGCCAGAGAACAATTGCAACAATTAGCAAAACAAACTCAAACAGATTTCTTTACCGCAGAAGAAAACAATGCAATTAATATTGCTCAAAAAGCAATAGAATTGGCAAAGGAAAACAAAAACGATTTAGTAATAATCGATACTGCCGGAAGATTGGCAATTGATGAAAATTTGATGCAAGAACTAAAAGATATTAAAAAATATACTCACCCTGATTATATATTTCTAGTAGTTGATGCAATGAGCGGACAAGATGTTATAAATACAGCAAAAATTTTCCACCAAGAACTTAATTTAGATGGCACTATTATTACAAAATTGGATAGTGATGCTCGTGGTGGCGCAGCTTTGTCGATTACTCATTTATTGAACGTTCCAATAATTTTTATTGGTACTGGTGAAAAATTAACTGGTTTAGAATTATTTCATCCAGATAGAATGGCAGATAGAATATTGGGCATGGGCGATGTTCTAACATTAATTGAAAAAGCTTCGGAAGAAGTTGATGAAAAAATGATGAAAAAAATTGGCTATAAAATGCTTTCTGGCCAATTCAATTTAATGGATTTGATGAATAGTTTAGCTCAAATTAAAAAACTAGGTAAGATGAAAGCAATCTTAAGAATGATTCCCGGAATGGCAGATAAGGTTTCAGACGAAAAGATTGAAGAAGCAGAAAATAAATTTAGAATTTATACTTATTTAATTAATTCAATGACGAACTTCGAGAAAAAAAACCCACGAGTTTTAAAAAACCCAAGTAGAAAGCAAAGAATTTTGCAAGGTTCTGGTAGAAGCCCAAGAGAATTTAATATGCTAATTTCGGATTTCGAAAGAATGTCTAAGCAAATGAAAGAATTAGCAAATGGAAATTTAAAATTAAAAAATGGCTTGGATGAATAAAAAAAGTGCCTATTGTTTGGCACTATTTTTTAACTAAATAATTAGCCATTTAAAATATAAAATTGCAAAATAATAACTAAAAAAATTCTTTAAAAAATAAAAAGCACTTATGGGTGCTTTTTATAATTTATTCTTATTTTTAATTGAAATTAAGACTAATATTGCTTTAGATATTTTTAAACTTGATAAAACATATCTATCAAAAATAATAATATTTCTTAATTTAAATATTGAATTTAATTTTTTAGTTTTTACAATTAGGTTATATGGAGAAATAGTTTTAATTTTATTGATGAAGTGATTAAATAAATTATTTAAAAAATATGAATGGTTTTTAAATGTTTCTAAAATATATTTGATATTTTCAGAGGCTATTTTTTTATCGCGAGGTTTTAATTCTTGACAATTTTGTTTTGAAAAATAAGTATTTAAAATGAAATTGATTGTTTCTTGTATTTCTTCATCTCTTGAAATTTCAATAAATGCCTTATCTAATAAATAAATTAAATTGTATTTATTATTTACTATTAAATTAGTTCATAATTCATCAAAGGTTTTTAATTTCGCATAAATATCGCTATAGTTTAAAAGTGTTTCTACTATAAAATAAATAAATTCCTTAAATGTATTCAATTGAGACAAAAATTTCTTAATTTCTATATATTTTTGATTATCGTTGAATGAAAATAAAAACATTAGCAAATCTGTATTTAATGATTCTTTTGCAAACGTTAGAGCAATTTTTTTAATTTCTTCTTTGTTTTCTTCTATTATTCCTGCACTAAAGAATTGTTTAAATACATCATATATTAAATATTGATAATTGCCTAGAATATAATTTCATTGTTCACGCAAATCAAAATTAATAATTGTTTTTTTAGTTAATAATACATCTTCAAGTCTTGATTGTAATCTAAATAATATATCATCAATTTTTCCATCTTTAAATAATGTTAAAAATATAGCTTTGCTATTTGTTTCGTTGCTTAAAAATGCATCAATCAACTTATAAAATTCATACTTAGTTTTTGCACCAAAAAATTTATTTTTTAAGTAATGTCAAGCAATGTTGTGAATATCATAAGAGGAATATCTATTTATTAATTGAATAAATTCTGAATTATTTAAATGTTTATATAAATATCTTTTTAGAGATATTTCTAATTTGCTATCTATTAATATATTGAAATTCTTATTAATTCCATAGATATTTTCTAGTAGGTCTAAATCATTGTTGTATAGTGAGAATAATTGTTGATCTTGGTTTTCAAGTGTTTGATTTCAATATTCAGAATCACTACAATATTGAATTAAATTAGACATTTTAAATTCACTAGTTAAACTTAATTTAGCAAGTAAATCATATGCGACTTTTTTATATCCTTTTTCGGTGCAAAACCAACTAAATAAGTTTTCATACAAATAATTTTGATGTTCTTTTCAATATTTTTCTTCATAAATATCTACATATAAACATCGGCATTTTTTAGCTACTTCTTTAGCGACATTGTTTAATAAATCTTGTATCATTTCAACCAAATCAAATTCATTGTTATATGAAGAAGAAATGTAAATCGAATTAATTAAATCTTGTAAGGCCATAAATGGTTTGGCATAATTTGTTACCACGATATTTGCTAGAGGATTAATTTTTTGAATATATTCAACAAGCTTTTGATATTTTGAAATAATTTCTTTGCCGATATTTTCAAAATCTTTTTTTATTTCAATAATTAAATTGGTTTTTGTTTCATTTTTTGCTGTTATTAAATTAGTTAATTTATAAAATGGCATTTTAAAAATTAAATCGCTAAATCCAGCTGTAATTGTTATAAAATTTGCCTTTGAAATAGTTTCTTTATAAAAAGAAAAATTTTTTTTAGAAATGTTTCAATTTAAAAAAAATTCATCAAAAAAATTCTTTGAAGGATTTGAAACATGCCAATCAATTGATTGAAGCAAATCAAGACTTTTTGAATTGGCAATTAAATCATTTTCATTATTTGAAGTTAAGGCATAAATTATGTCTAAATTACCTGCTGGTATTGCCAGATTGTAAAAACTTGTTAATCTATAGTTTAAATCTCGCAATTTTAAGGCTAAAAATGATGGATAACCCAAACCATCTATATTACCATTTGATAATTTTCCATTAGTATTAAACCCTACTTTGCTATTAAATCCCGCTGCAAATGAATCTCCTAAAGCAACATATTTAATTTCGTCATTTAAATTGTATTTTTGATTTTTAGCCATTTTGTTCCTTTTCTTGACATTGATCATAAACAATATTATTTAGATTATTAAAATAAGAGATTAATTGAACTATTGCTTTTTTTACATCGGGTAATTGATTTTTTAATATTCAATTATTATTTTTAGCATATAAACTCAATTGGCTAAAGATTTCTTCTTCATCTTGACTTGGTTGCAATTTTATATCAATAATTTTTTGAGGATAATACTTAGATATTAGGCGGTAAGTAGCAACAATAACTTCATTTATATTTAAAGCTTCCAACTTTATTGAACTAACTACAGCTAATTTGGCGCCGGTTTCTTCATCTTTTAGTTTTGGCATTAAAAGTCCTGGTGTATCTAAAAGTAAATAATTTTTGCAATTTATTCATTGCTCTGACTTTGTTACTCCTGCCATTGCACCCACTTTTGCAACTTTGGAACCAGCCAATAAATTTATTAAGGTACTTTTACCAACATTAGGAACGCCAAGCACGACACATTTTAATGCTGGTGTTAGCTTATGTTTTTGCGCATTTTTTTCTTTTACTTTTAAATAAATATTATTAAGAGCTGAATTTATTTTTTTATAGGCTAATTTATCTTTTAAATTAGTTGTTACAACAACACCTTTCTTTTCATATTCATTTATAAAAGGTTTTAATTTAGATAGATCTGCTTTGTCGCTTTTGTTTAATATGAACAAAATTGGCTTATTATTAGCCATCTTATAAATATCGTTATTAAACGAAGTAAGAGGTAAGCGTGCATCTAAAACAATTATAAATAAATCAAATAATCTTATTTTTTCATTTAATAATCTAATGGTTTTGGCCATATGACCAGGGAATCAATTAAACATAAAATTTATCAATCTTTCCTTAAAATAAAATCCTTCCCTTTTTGACTTTCAAATTTTGATAGTCCGGCGAAATCATTTTGTCTTAATACTTCAAATAGTACTATTGCAACACTATTAGCTAAATTCAAACTACGACATTCATCAAACATCGGAATACGCAAACAATTAGGAATATTTGTTTGCATAATTCTCTTAGGAATTCCTGTGCTTTCAGTTCCAAACATAATTCAAAGTTCACCATTTGTTTGCAATTCTTGTTTGTAATCAACATCTACGTAATTTTTTAAACCGTATCTTGTTACATAATAAATATTTTTATCACCATATTTTTTAAAAAATTCTTCATAATTTTCATGAACTTCATGTTGAATTTCAGTTAAAAAGTGTCCAGCGGCCTTTCTTTTCATTCAATGAGGATGAAGATCAAAGGCAATTGGTTTAATAATATGCAATTTTGCATTGCCGGCAAAGCATGTACGAATTATATTAGATGTATTTGGGCTTATTTCGGGTTGGTATAAAATAATGTTAATCATGTAAAGTATTTTACTACAAATTAGCATTAAAAATAGTAGATATTGTAATTTTAATTATTTAAATAATTAAAAAAAATTATATAGATCAAATTCAAAAAAGCAGATTTTAAAAAATTTATAGCTAAATATTTATTTATGTTGTAGCCACATCTTTATGTTAATATATAAAACAAAATAATTACAGAAAATAAGAAAAAATAGCGAAAAAATCGCTTTTTTTCTAAACTAAAATAAAAAGCACATTCGTTGTGCTTTTTATTTGCTTTATTTTCTGATGTTTAAAGCTTTGATGGTTTCTAAATATAAGTTGTAGTTTTCGTCTTTTAAGTGATTTAGTAATGCTTTACGGTGATTAACTTTAGCAATGAATCCTCTCATTGAATGTAAATCTTTTTTATTTACTGCAAAATGTTCTTTTAAGCTTTCAATGTCTTCAGTTAAAATTGCTATTTGTACTGGTAAATATCCAGTGTCTTTTGCATTACGTCCGAATTTAACAACTAATTCAGCTTTTTTTTCTTTTGATACCATATTTTTCCTTTCATAGCCTAGCAATAATCTTAATTATTACCAAGGGATAAAATTGTTATGAATATATTTTAACATAAAAATAAAAAAAATACGCAAATGCGTATTTGGTCAAGTTAATGAAATGGTGCGAACGAATGGACTTGAACCATCGACCTCACGATTATCAGTCGTGTGCTCTAACCAACTGAGCTACGCTCGCATATAAGGAGTTTTAAAAAACTCCATAATATTATAACACAAAAACAAATTAACGTTTTGAAAATTGTCTTGCACGTCTTGCTTTTCTTAAACCGAATTTCTTACGTTCTTTAACTCTAGCATCACGTGTTAAAAATCCTGCATCTTTTAATTTATTTCTATATTCGTTATTAGATGCTTCTAATAATGCTCTAGCAATACCTAATCTAATTGCTCCAGCTTGTCCTGTTAAACCACCACCATTAACATTTGCAAAAACATCAAATTGGTTAGCAGTTTCAGTTACTGCAAAAGGACTTAAAGCATCTTTTATTAAAATTTCTGAATTTAAGTATTCTTTTGCAACTATTCCATTAATAATTAAATTTCCACTACCTGGTAAAATATAAACTCTTGCTACTGAAGATTTACGACGACCTAAACCATAGTATCTTACTTTATCTGCCATAATTATTTAACCTCTAATTTAACTGGTTGTTGTGCTTCTTGTTTGTGTTCAGAACCTGCATAAACATATAAATTTTTAAATTGTTTACGACCTAATTTGGTGTGTGGAAGCATTCCTCTAACAGCTTTTTCAACTAATAATTCTGGTTTTTTTGCTCTTAAATCAGCAGCGGTAATTTGTTTTAATCCACCTGGGTATCCAGTGTGGTGGTAATATACTTTTATTTCATCTTTTTTAGCAGTTAAAAGTACATCTTTAGCATTGATCACAACCACATTGTCGCCCATATCAACATTAGGGGTAAAAGTTGGTTTATTTTTACCTCTAAGTACAGAAGCTACAAGTGCTGAAAGTCTACCTAAAGGTACGTTGGCTGCATCGATAATGAATCATTTTTTATCAACGATTTCTTTTCTAATAATTGTTGTTTGACGCATACTTTCTCTTTCTTATAGTTTGTTATTTTTATAAAATGCTTACCTATTATACCATATATAGTTATTATTAAAACATTTTTATTTAAGTTTTAAATATTTAAATAAAACCTGTTTAATTTAATTTTGAAAGCATATAAATTAAGGCGGTCTCAGCCCTTAAAATCGTTTTTGTTAGTGAAACAACTTGAACATTACAAGATATGGCATAATCAATTTCTTGTTGGCTAAATCCACCTTCAGGGCCAACAATAATTAAAGCATCTTGGCGATCTTTAATTAATTCGTTATTTTTTTTATTTTCATAAGCTAATACTTTATTTTTAATATCCAAGACACAAAGTTCTGAAAATGAAATTGGATCATGAAGTCTTGGAATATAATTTCTAAAACTTTGTTGGCAAGCTTCAAAAATTATTTTTTCATAACGTTGCTTTTTAGAACCAAAATTTAAATTAGATTTATCAACATATTGGCTTTGAAATATATAAATTTCATAAGCGCCAAGTTCAACTGCTTTTTGCAATGCTATTTCAAAATGGTTTTGTTTAATTAAAGGAATTGCAACTGAAATTTTATAATCCAATTCATTATTAATATCTAACTCTTTAACTAATTTAGCACTATTAGGAAAAACAAATTGACATTCATAAAACTTATTTTCATAATTTACAATAATATTTTCTTTGTTAAAACGTAAAACCTTAAAATGCTTTTGGATTTCATCACTAAAATAAAAAATTCCATCTTCTAATTTGTTGGCAAAAAATTTATACATATAACTATTTTAATATAACCAACACAATAAAACAAAAAAACCCGTAACTTTTTTATAAATTACGAGTATAAATTTTTTGACTAATAATTATTATATGTAATCTAATTGCTAAAAATTATTTTATTAATAATTAAAATTAATTGTTAGATTAAATAATTTTTGTCTTTGTTTGGTCTATACCAATATATTGTCAATTTATTTCACTTGCATAGCCCTTATAAATTACTTCTTTAAGGTTTTCACATCTAGAAAATGCACCTTCACCAATTCCTTTTATTGAACTAGGAATAGTAATAGATTCAATTTTTGTATTATAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCAATCATAAAACGCTTTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGTATCTGAAAATGCCCTAACTCTAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTCTTAAGGTTTTCGCAACCAGAAAATGCAACTTCACCAATTTCTTTTATTGAACCAGGAATAGTAACTTTTGTGACCTTTTTATCCAAATATGCTAATATCTTTTTATTATTTTTATCAATAAAAAAATTGTCTCTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATCTGAAATCATTGAAAAATCAAATGCTCGATTGCCTATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTGTATTTCAAAATGCATCTGCGCCAATTTTTTCAAGACCTTCATTTAAAGTTACTTTCTTAAGGTTTTCGCAACCAGAAAATGTACTTTTGTCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTGTTTTTGAAAATGCCCAAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTCATCAAGCATAAAACGCACTTTCGCCAATTTCTTTTACTGAACCAGGGATAGTAATAGATTCAATGTTTGTACCAAAAAATGCCCGAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCAACCAGAAAATGCACCTTCGCCGATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTACCACCAAATTCCCCAACGCCAAATGCATCTGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTCTACAACCAGAAAACGCTCATTGGCCAATTTCTTTTACTGAACTAGGAATAGTAATAGATTCAATATTTGTATCATAAAATGCACCAGCGCCAATTTTTTCAAGACCTTCATTTAAAGTTACTTTCTTAAGGTTTTCGCAACCAGAAAACGCACCTTCACCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTGCTTGAAAATGCACCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTTGCAACCAGAAAATGCACCTTCACCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTGCTTGAAAATGCACCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTTGCAACCAGAAAATGCACCTTTGTCAATTTCTTTTACTGAGCTTGGAATAGTAACTTTTGTGACCTTTTTATCCAAATATGCTAATATCTTTTTATTATTTTTATCAATAAAAAAATTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTAAAATACTTGAAATATTTCCACTAGATAATGCGCCTTCGCCGATTTCTTTTAATGAACCAGGAATAGTAATAGATTCAATTTTTGTATTATAAAATGCCTCTGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCAACCAGAAAACGCACCTTCGCCGATTTCTTTTGCTGAACCAGGAATAGTAACTTTTGTGGCCTTTTTATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATGTGAAATTATTGAAAAATCAAATGCTCAATTGCCTATTTCTTTTACTGTATCAGGAATAGTAATAGATTCAATTTTTGTACCAAAAAATGCCCGAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTTCTAAGGCTTCTGCAACCAGAAAACGCACCTTCGCTAATTTCTTTTACCGAGCTTGGAATAGTAATAGATCAAATGTTTGTACTTCTACTAAATGCCCGAGCGCCAATTTTTTCAAGACCTTCATTTAAAAATGCTCTTGTAAGGTGTTCGCAACCAAAAAACGCACCTTCACCAATTTCTTTTACTGAACTAGGAATAGTAATAGATTCAATGTTTGTGCTTGAAAATGCACTTGCGCCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTGTTTTATAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCAACCAGAAAATGCACCTTCACCAATTTCTTTTATTGAACCAAGAATAGTAATAGATTCAATTTTTGTTTTATAAAATGCCTCAGCACCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCAACCAGAAAATGCACCTTCACCAATTTCTTTTATTGAACTAGGAATAGTAACTTTTGTGGCCTTTTTATCCAAATATGATAATATTTTTTTATTATTTTTGTCAATAAAAAAGTTATCTTCAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATCTGAAATCATTGAAAAATCAAATGCTCAATTGCCTATTTCTTTTACTGTATCAGGAATAGTAATAGATTTAATGTTTGTATTTGAAAATGCCCGAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGATTTTGCAATCAGAAAACGCACCTTCACCAATTTCTTTTACTGAACTAGGAATAGTAACTTTTGTGGCCTTTTTATCCAAATATGATAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATCTGAAATCATTGAAAAATCAAATGATCAATTGCCTATTTCTTTTACTGTATCAGGAATAGTAATAGATTTAATGTTTGTACGATCAAATGCCCGAGCGCCAATTTTTTCAAGACCTTCATTTAAAGTTACTTCTTTAAGGTTTTCACATCTAAAAAATGCACCAGCGCCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTTTATCAGCAAATGCACTTTCGCCAATTTTTTCAAGACCTTCATTTAAAGTTACTTTCTTAAGGTTTTTGCAACCAGAAAATGTACTTTTGTCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTGCTTGAAAATGCACCAGCGCCAATTTTTTCAAGACCTTTATTTAAAATTACTTCTTCAAGATTTTCACAACCAGAAAACGCACTTTCACCAATTTCTTTTACTGTACCAGGAATAGTAATAGATTCAATGTTTGTATCAGAAAATGCCCAAGCGCCAATTTCTGTAATATTTTTGCCTTGATATTCACTAGGAATTGTTACTCTAGAATCATTTCCAATATATTTTACAATTTTTCAAGTATTTGATTTAGTTAGTTCAAATTTAAAAGTTTTATTTAAATTTGATGAATCTTCTTCTTTGGTAGGATTTTCTGGTTTAGGTTCGGGTTTAGGTTCGGGTTTAGGTTCTGGTTTAGGTTCTGGTTTAGGTTCTGGTTTAGGTTCGGGTTTAGGTTCTGGTTTAGGTTCTGGTTTAGGTTCTGGTTTATTTTCATCGGGTTTTAAATCCTCTTTGTTTTCTTTTGAATTTTGTTTTACTCCATAATCACAAGAAATAGTTGCTAATGGAAGCATAGCAGTTAATGCTGAAATACTACTTAATATAAGTCATATCTTACTATTTTTTTTATTTTTCATTTTCTTACCTTTCATTACTATTTATAATTTTATTTTTATATATATTAAAAAGTAAAACCTCTTAATAAATAAAAAAAAAAAAAAAAAACAGATTTTTAATATAATTGGTTGAAAAAATGGCTTATTTTCTATATTTTTTATGCAATTTATATATTATTAAGGTCAAAAAAAATATATAAAAAAACTCAGGTTGAACTGAATTTTTTTATTTTTTAAATGGCAATAAAGACAATTAATTGCATTTTTTTAAAACAGATTTCTTACATCATCTTAGTGTTTTAATATATCTGCAAGTTCATCGTTTGAATCTTCATCGTTATCCATTGAAAAAAATGATTGTGTTTTAAATGTTCCTTCAGATACAAAGAATTCCTTTGTTTCATCGTTAACATAATTTAAATCGTCTTTTTTTATTACATTGATTACTTCATCTTGAATTTGATCAATGTCTTTAGGAGCGTCTTTTCTTAATCCCGTGGCGATTACCGAGACAAATAGCTCACCTAATTTGTTGCTTTCTTCAGATTCTAGAGTTGTTATACCAAAAATAATGTTAATTTCATTACCAACAATTTCCCTCATAGCTCCCAAAGCACTTTGAATTTCATATTAAAATAAAAAATCCGTCTTCTAATTTGTTAGCAAAAAATTTATACATATAAGCATTTTAATATAACCAACACAATAAAATAAAAAACTCGTAACTTTTTTATAAATTACGAGTATAAATTTTTTACTAAATATTAATTATATGTAATCTAATTGCTAAAAATTATTTTATTAATAATTAAAATTAATTGTTAAATGAAATAATTTTTATCTTTGTTTTGTCTATACCAGTATTTTCTCAATTTATATTACTTGCATCACCTTTATAAATTACTTCTTTAAGGTTTTTGCAATTATAAAACGCACCTTTGCGAATTTCTTTTACTGTATCAGGAATAGTAATAGATTCAATTTTTGTATATTCAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTCAAGGTTTTCGCAACCAGAAAATGTACTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGTATCATAAAATGCCCAAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTCAAGGTTTCTGCAACTAGAAAACGCACTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAACTTTTGTGGCCATTCAATCCAAATATGATAATATTTTTTTATTATTTTTGTCAATAAAAAAGTTATCTTCAATTTCAAAATTTTTATTATTTGAATTAATTAAAATACTTGAAATATTTCCACTAGATAATGCACCTTCGCCGATTTCTTTTAATGAACCAGGAATAGTAATAGATTCAATTTTTGTATCAAAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTCAAGGCTATGGCAACCAGAAAATGTACTTTTACCAATTTCTTTTACTGAACCAGGAACAGTAATAGATTTAATGTTTGTATTTGAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTTATTTAAAATTACTTTCTTAAGGTTTTTGCAACCAAGAAATGTACTTTTGTCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTATTGCTAAATGCCCAAGCGCCGATTTTTTCAAGACCTTCATTTAAAACTACTTTTTTAAGGTCATAGCAATCATAAAACGCACTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGTATCTGAAAAAGCACTTTCGCCAATTTCTGTAATATTTTTGCCTTGATATTCCCTAGGAATTGTTACTTCAGAATCATTTCCAATGAATTTTGCAATTTTTCAAGTATTTGATTTAGTTAGTTCAAATTTAAAACTTTTATTTAAATTTGATGGATCTTCTTCTTTGGTAGGATTTTCTGGTTTAGGATTTGGTTTATTTTCATCAGGCTTTGAATCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTTTATGATCGCAAGAAATAGCTGCTAATGGAAGTATAGTAGTTAATGCTGAAATACTACTTAATATAAGTCATATCTTACTATTTTTTTTATTTTTCATATTTTTTTCCTTTGTTATATTTAATTATTATATTTTATATATCTATTTAAAGCAAATAAATTATTAAAAAAATAAAAAATCTTTTTTTTGAATGAATTTGGAAGCGTAGAAAAATGTGGGGTTAATTCCAAATAAAGAAAAAATAAGTTTAAAAACTTAAAAATTTTTGTAAGAAATTTAAATTGGGAGAAAAATTATAGTAAAAAAATCTATGACAAATTAAGCAACATAGCTATAAACAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATAGCTATTAAATCCTTGAAAAAATTCCATCTTCTAATTTGTTGGCAAAAAATTTATACATATAACTATTTTAATATAACCAACACAATAAAACAAAAAAACTCGTAACTTTTTTTTATAAATTACGAGTATAAATTTTTACTAAATAATTAATTATATGTAATCTAATTACTAAAAAATTATTTTATTAATAATTCAAATTAATTGTTAGATGAAATAATTTTTATCTTTGTTTTGTCTATACCAATATTTCTTCAATCTATTTTACTTGCATCACCTTTATAAATTACTTCTTTAAGTTTTATACAACCAGAAAATGTACCTAAGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTTTGATTAAATGCCCAAGCGCCGATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTGGCAATTAGAAAACGAACCTCTGCCAATTTCTGTTACTGAACCAGGAATAGTAACTTTTGTGACCTTTTTATCCAAATATGCTAATATCTTTTTATTATTTTTATCAATAAAAAAGTTGTCTCTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATGCAAAATTGTTGAAAAATCAAATCCACCAACGCCAATTTCTGTTACTGAACCAGGAATAGAAATAGATTCAATGTTTGTACCATCAAATGCCCAAGCGCCGATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTTGCAATCAGAAAACGCATAATCGCCAAATTCTTTTACTGAACCAGGAATAGTAATAGATTTGATGTTTGTCTTTGAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTCTTAAGGTTTTTGCAATCAGAAAACGCTCTTTTGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTACCATCAAATGCCCAAGCGCCAATTTTTTCGAGACCTTTATTTAAAATTACTTCTTTAAGGTTTTTGCAATTACAAAACGCACTTTCGCCAATTTCTTTTACTGTGCCAGGAATAGTAATAGATTCAATTTTTGTATCATAAAATGCATAATCGCCGATTTTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTGGGCAACCAGAAAACGCACTTTCGCCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTGTATCTCTAAATGTCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTGGGCAACCAGAAAATGTATTTTTGTCAATTTCTTTTACTGAACTAGGAATAGTAATAGATTCAATTTTTGTATCTCTAAATGTCTCAGCGCCGATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTGGGCAACCAGAAAATGTATTTTTGTCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTATCATAAAATGCCCTGGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGCCTGTGCAACCATAAAACGCACCTTCACCAATTTCTTTTACTGAACCAGGAATAGTAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATCTTTTTATTATTTTTGTCAATAAAAAAATTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTAAAATACTTGAAATATTTCAACTAGATAATGCGCCTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTTGATGTTTGTCTTTGAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTCTTAAGGTTTTCACAACCAGACAATGTATTTTTGTCAATTTCTTTTACTGTGCCAGGAATAGTAATAGATTCAATTTTTGTATTATAAAATGCCCCAACGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGCCTGCGCAACCATAAAACGCACCTTCACCAATTTCTTTTACTGAACTAGGAATAGTAATAGATTCAATGTTTGTATCTTCAAATGCCTCAACGCTAAATGCCCTAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTTGCAACCAGACAATGTATTTTTGTCAATTTCTTTTACTGTGCCAGGAATAGTAATAGATTCAATTTTTGTATTATAAAATGCCCAAGCGCCAATTTTTTCGAGACCTTTATTTAAAATTACTTCTTTAAGGTTTTTGCAACCAGAAAACGCACCTTTGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGCATATTCAAATGCCTCAGCGCCGATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTGGGTAACCAGAAAATGTATTTTGGTCAATTTCTTTTACTGAACTAGGAATAGTAATAGATTCAATTTTTGTATCATAAAATGCCCTGGCGCTAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGCCTGTGCAACCATAAAACGCACCTTCACCAATTTCTTTTATTGAACTAGGAATAGTAACTTTTGTGACCTTTTTATCCAAATATGCTAATATCTTTTTATTATTTTTGTCAATAAAAAAATTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTAAAATACTTGAAATATTTCAACTAGATAATGCGCCTTCGCCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTGTATCACAAAATGCCCCAGCGCCGATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGCCTGTGCAACCATAAAACGCACCTTCACCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTGCGAGTAAATGGCCCAGCGCTAAATGCCAAAGCACCAATTTTTTCAAGACCTTTATTTAAAATTACTTCTTTAAGGTTTTTGCAATCAGAAAACGCACCTTTGTCAATTTCTTTTACTGAACCAGGAATAGTGATAGATTCAATTTTTGTATATTTAAATGCCTCAGCGCCAATTTCTGTAATATTTTTGTCTTGATATTCCCTAGGAATTGTTACTTTAGAATCATTTCCAATAAATTTTGTAATTTTTCAAGTACTTGATTCAGTTAGTTCAAATTCGAAAGTTTCATTTAAATTTGATGAATCATCCTCTTTGTTTTCATTTGAATTTTGCTTTACTTCATAATCACAAGAAATAGTTGCTAATGGAAGTATAGCAGTTAATGCTGAAATACTACTTAATATAAGTCATATCTTACTATTTTTTTTATTTTTCATTTTCTTACCTTTCATTATTATTTATAATTTTATTTTTATATATATTAAAAAGTAAAACCTCTTAATAAAAAAAAAAAAAAAAAACAGATTTTTAATATAATTGGTTGAAAAAATGGCTTATTTTCTATATTTTTTGTGCAATTTATATATTCTTAAGGTCAAAAAAATGCAATAAAAAAACTCAGATTGAACTGAATTTTTTTATTTTTTAAATGGCAATAAAGACAATTAATTGCATTTTTTTAAAACAGATTTCTTACATCATCTTAGTGTTTTAATATATCTGCAAGTTCATCGTTTGAATCTTCATCATTATCCATTGAAAAAAATGATTGTGTTTTAAATGTTCCTTCAGATACAAAGAATTCCTTTGTTTCATCGTTAACATAATTTAAATCGTCTTTTTTTATTACATTGATTACTTCGTCTTGAATTTGATCAATGTCTTTGGGAGCGTCTTTTCTTAATCCCGTGGCGATTACCGAGACAAATAGCTCACCTAATTTGTTGCTTTCTTCAGATTCTAGAGTTGTTATACCAAAAATAATGTTAATTTCATTACCAACAATTTCCCGCATAGCTCCCAAAGCACTTTGGATTTCATCACTAAAATAAAAAATTCCATCTTCTAATTTGTTTGCAAAAAATTTATACATATAAGCATTTTAATATAACCAACACAATAAAATAAAAAACTCGTAACTTTTTTATAAATTACGAGTACAAATTTTTTACTAAATAATCAATTATATGTAATCTAATTGCTAAAAATCATTTTATTAATAATTCAAATTAATTGTTAGATGAAATAATTTTTATCTTTGTTTTGTCTATACCAATATATTCTCAATTTATTTTACTTGCATCACCTTTATAAATTACTTCTTTAAGGTTTTTGCAATTATAAAACGCTCTTTCGCCAATTTCTTTTACCGAACCAGGAATAGTAATAGATTCAATGTTTTTACCAGCAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGCTTTCGCACCAAGAAAACGCATATTTGTCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTATTTGAAAATGCATATCAGCCAATTCCTTCAAGGCCTTCATTTAAAATTACTTCTTTAAGGTTTGTGCAACCATTAAACGCATCTTCGCCAATTTCTTTTACTGAACCGGGAATAGTAATAGATTCAATTTTTGTATCGCTAAATGCCCAAGCGCCAATTTTTTCAAGACCTTTATTCAAAATTACTTCTTTAAGGTTTTTGCAACCATAAAACGCTCATTCGCCAATTTCTTTTACTGAACCGGGAATAGTAACTTTTGTGGCCTTTTCATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCGAAATTTTTATTATTTGAATTAATTGAAATATGTGAAATTCTTGAAAAATCAAATGCCCTGACGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGTATCTAAAAATGCCTTAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTGTGCAACCAGAAAATGCACTTTCGCCAATTTCTTTTACTGTATCAGGAATAGTAATAGATTCAATGTTTGTATATGAAAATGCACAAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTCTTAAGGTTTGCGCAATCAGAAAACGCACCTGCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGGATTATTAATATTAATTAACCCAGCAAATGCCGAATAGCCAATTTTTTCAAGACCTTTATTTAAAATTACTTCTTTAAGGTTTTCGCAACCATAAAACGCTCATTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTTTTATAAAATGCCTCAGCACCAATTTTTTCAAGACCTTTATTTAAAATTACTTCTTTAAGGTTTGGGCACCAAGAAAACGCTCTTTCATCAATTTCTTTTACTGATCCAGGAATAGTAACTTTTGTGGCCTTTTTATCCAAATATGCTAATATCTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATGTGAAATTATTGAAAAATCAAATGCCCAAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCATGAAGGTCTCCGTGGCCTAAAAACGCACCTTCGCCAATTTCTTTTATTGAACTAGGAATAGTAACTTTTGTGGCCATTCAATCCAAATATGATAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTAAAATAAGTGTAGTCATTCAAAAATCAAATGCTCAATTGCCAATTTCCTTTACTGAACCAGGAATAGTAATAATTCCAATTTTTGTATATCTAAATGCTTCAACGCCAATTTTTTCAAGACCTTCATTTAAAATTGCTTCATTAAGGTTTGTGCAACCATGAAACGCTCTTTCGCCAATTTCTTTTACTGAACTAGGAATAGTAATAGATTCAATGTTTGTTTTATAAAATGCCTCAGCACCAATTTTTTCAAGACCTTCATTTAAAGTTACTTCTTTAAGGTTTTCGCAACCAGAAAATGCACCTTCACCAATTTCTTTTACTGAACTAGGAATAGTAACTTTTGTGGCCTTTTTATCCAAATATGATAATATTTTTTTATTATTTTTGTCAATAAAAAAGTTATCTTCAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATGTGAAATGTCTGAAAAATGAAATGCCCCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCAACCAGAAAATGCACCTTCACCAATTTCTTTTATTGAACTAGGAATAGTAACTTTTGTGGCCATTCAATCCAAATATGATAATATTTTTTTATTATTTTTGTCAATAAAAAAGTTTTCTTCAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATCTGAAATCATTGAAAAATCAAATGCTCAATTGCCTATTTCTTTTACTGTATCAGGAATAGTAATAGATTTAATGTTTGTATTTGAAAATGCCCGAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGATTTTGCAATCAAAAAACGCACTTTCGCCAATTTCTTTTACTGAACTAGGAATAGTAACTTTTGTGGCCTTTTTATCCAAATATGATAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATCTGAAATCATTGAAAAATCAAATGATCAATTGCCTATTTCTTTTACTGTATCAGGAATAGTAATAGATTTAATGTTTGTATTTGAAAATGCCCGAGCGCCAATTTTTTCAAGACCTTTATTTAAAATTACTTCTTCAAGATTTTCACAATCACAAAACGCACTTTCGCCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTTTATCAGCAAATGCACTTTCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTCTTAAGGTTTTCGCAACCAGAAAATGTACTTTTGTCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTTTTATAAAATGCCTCAGCACCAATTTTTTCAAGACCTTCATTTAAAGTTACTTCTTTAAGGTTTTCACAACCAGAAAATGCACCTTCACCAATTTCTTTTACTGAACTAGGAATAGTAATAGATTCAATTTTTTTATCAGCAAATGCACTTTCGCCAATTTTTTCAAGACCTTCATTTAAAGTTACTTTCTTAAGGTTTTCGCAACCAGAAAATGTACTTTTGTCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATGTTTGTGCTTGAAAATGCACCAGCGCCAATTTTTTCAAGACCTTTATTTAAAATTACTTCTTCAAGATTTTCACAATCACAAAACGCACTTTCGCCAATTTCTTTTATTGAACCAGGAATAGTAATAGATTCAATTTTTGTATCATAAAATGCCCAAGCGCCAATTTCTGTAATATTTTTGCCTTGATATTCCCTAGGAATTGTTACTTCAGAATCATTTCCAATATATTTTACAATTTTTCAAGTATTTGATTTAGTTAGTTCAAATTTAAAAGTTTTATTTAAATTTGATGAATCTTCTTCTTTGGTAGGATTTTCTGGTTTAGGTTCTGGTTTAGGTTCGGGTTTAGGTTCTGGTTTAGGTTCGGGTTTAGGTTCTGGTTTAGGTTCTGGTTTAGGTTCGGGTTTATTTTCATCGGGTTTTAAATCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTCCATAATCACAAGAAATAGTTGCTAATGGAAGTATAGTAGTTAATGCTGAAATACTACTTAATATAAGTCATATCTTAATATTTTTTTATTTTTCATTTTCTTACCTTTTATTACTATTTATAATTTTATTTTTATATATATTAAAAAGTAAAACCTCTTAATAATAATAAAAAAAAAAAAAAAAACAGATTTTTAATATAATTGGTTGAAAAAATGGCTTATTTTCTATATTTTTTATGCAATTTATATATTCTTGAGGTCAAAAAAAATATATAAAAAAACTCAGATTGAACTGAATTTTTTTATTTTTTAAATGGCAATAAAGACAATTAATTGCATTTTTTTAAAACAGATTTCTTACATCATCTTAGTGTTTTAATATATCTGCAAGTTCATCGTTTGAATCTTCATCATTATCCATTGAAAAAAATGATTGTGTTTTAAATGTTCCTTCAGATACAAAGAATTCCTTTGTTTCATCGTTAACATAATTTAAATCGTCTTTTTTTATTACATTGATTACTTCATCTTGAATTTGATCAATGTCTTTGGGAGCGTCTTTTCTTAATCCCGTGGCGATTACCGAGACAAATAGCTCACCTAATTTGTTGCTTTCTTCAGATTCTAGAGTTGTTATACCAAAAATAATGTTAATTTCATTACCAACAATTTCCCTCATAGCTCCCAAAGCACTTTGAATTTCATTTAAAGTTACCTTTTGAGAAGCACTGAAATTTACTATTGCATCACTAGCCCCAACAACACTTGATTCTAAAATTGGGCTTGTTATTGCAGATGTGATTGCTTTTACAGCCCGATCTTGGCCATTTGCTTGGCCAATGCCGATTACTGTTTCACCTTTATTTTTAATAATGGTTGATAAATCTGCAAAATCAAGATTAATAATGCTTGGGGTGGCAATAACATCAACTATTGTTCTAATAGTTTGTTTTAAGACATTATTTGCACAAATAAATGCATCTGAAAAAGAAATATTTCCATATTGTTGTAATAGTTTATTGTTTGAAATAACAATATATGAATCAACACATTTTTTAATTTCTTGTAAGCCTTGCTTTGCAATTCTCATTCGCTTTGGACCTTCAAAGTCAAACGGAGTAGTTACAACCGCAACTACTAAAGCATTGCATTCCTTGGCTATTTGTGCAATCACGGGCGCTGCACCTGTGCCGGTTCCACCACCCATTCCTGCAGTTATAATTACTAGATCAGCTCCTTGCAATCTACTTTTGATTTCTTCACGCGAAGATTCAGCAGCAGTTTTGCCGATTTCAGGGTTAGCACCCGCGCCAATTCCACGTTCATCACCTAAATGTAAAACGCATTCTTGAGGAAATTTTGTTAATACTTGTTTATCGGTATTGGCAGCAATAATTTGAAAACTATCTAAATGGCTATTTATCATAGTTTCAACACTATTATTTCCACCACCACCTACTCCAATAACAATTATTTTTGCCACCGGGTTATAATTATTTAATTCTTTTGCCATAGAACTCCTAATTTATATCGTTTAGCATTAAATTGTAGTACTTTTTGGAATTACTATAAACTTCAATTGTAAATAATTCCCTACTAATTTCATTATTTAAAGCATTTTGCTTGATTTGACATTCTAAAATATTATCTATTGCAAGCATTTCGCAACTTAAATATTCATTTTTGATTTCATATTCAAAGTCACTAAATAAGTTAATTTTTTCATTTTGAATTAATATATTATCAAAAAGTTTTAATTCGCTGTTGAAATATAATTTGCAAACCTTTGAATCAATTTTATAATTATTAACAAAAGTTGCAACGCTAGTTAATAATTTATTTATAAATTCGCCTAATATATTCTTTATTATTGGAGCAAAATTGCATTTGTAGGTTAAACAATTTACAACTTCACTAAATCAATTGCTTTCTGTACTTAATAAATTACATTGTAACATTCTTGCTTTTAATTTGTTTAGTCCTAAATCTAATACTTGGCTTACTATATTTGATTTATTAATGTTTAAAATAAATTTAGCTTTATCATCTAATACATCAACAAAAATAGCTACTTTTTGTTTTGAATTTTTAAACAAAACTGAATTTGTCTTTAAACTATCTAAGCAAAATATTTTGGCAATTTTTAAATCGTTGCTACTAAATATTTCTTGCAATCTTAGACTATTAGCATTTGATATTGTTTTTAAAGAATAAACTTGCTTAATATTAGTTTTTTTAACTAGTTTAAATTCCAATATTTTTTGGCCTATATTATTGAAGTTTGATCTAATATTTTTTAAATTACTAATATTAAAATTGTCGTTATTTAAGGTTAATAGTTTACTATTTATATTTCATTTGCTATCTTCAGACAACATAACAATAACTTCATTTATGCTTGCTGATATTAATTTTTGAATTTCATTCTTTGTTTGCTTTATAAAATTTTTTATATCGCTTTCAGTTAAATAAATATTATTAATTGCTTTTTTATAAATGCAAAAAGATAAAGTATTAGTTTTTTCCATAACTTCTACTTCGATACTATTTTTTAAAAATTTATATGCAGCAATAATAATTTTTTTCATTTAACTAATCCATTAATTTTGTAATAACGCGTAATTTTGCGCTTCTTGATCTTTTGTTGATTTCTTCTTCTTTACTACTTGGAAAAATTACTTTTTGATGTCACTTATGAGTTTCAACAATTAGTATTTTTTTTAACTTAGGGTCAAAATAATTTAAATTTTGATAAAACTTTTTAACTATAACGTCTTCTTTACTATGAAATGTTATAATCGCTATTTTTCCGTCTTTTTTTAATAAATTAGGAAGTTGGCTTAGCATTTCACTTAAGGCATTTAGTTCATCATTCACTGCTATTCTAAAAGCTTGAAAAATTGCTTTAGAAGGATTTTTTTGTCTTACTATTTTAGCTGGCAATGAATCCTTTATTATTTGATTTAACGTAAATGAATCAGATATTGGTCGATTTTTTACAATAGCTTTCGCAATTAAATTACTTAATTTTACATCAGCATTAGATTGCAATATATCTATAATTTCTTTTTCAGTTGCATTATTAATAATTTCTTTTGCAGTTAGAGTATTATCTAAATCCATTCTCATATCTAAATCAGAATTGATTGAATAAGAAAATCCTCTATTTGGATCATCTAATTGAGGACTAGAAACTCCTAAATCTGCTAATATTCCATCGACTTTATCAATATTTAGTTTTTTTAGTTCACAAGTAATGTTTCTAAAATCACTTTTTATTAAAATAAAGTTTTGGCTTATTTTTATTAATTTTTCTCTACTTTCGTTTATAGCTTGAATATCTTTATCAAAACAAATTAATTTTCCAGTTGTTAGTCTTTTTAAAATTTCTTGGCTATGACCACCACGGCCTAATGTTAAATCAACATATATTCCGTCTGGTTTTATTGATAATTGTTCTATTACTTCATTTAATAGAACTGGAATATGTTCCATTTAGAACTTAACTCCTGCTTCAAATAATTTTTGAGCCAAGTCATTTACATTATCATCATTTTCATAGTAGCTTTCTTGTTCATCATAAACTTCTTTAGCAAATAATTCACAAGTATCACCAGTTCCTAGGAAAACAACTTCTTTGTCGATAGCGGCTTTATTTAAAAGATGTTTGGGAATCATGATTCTGTTATTTTTATCAGGAGATACAACTTCAGTATTTCCAAAAATGTAGCGAATAAAATCTCTTACGTTTTTGTTAAGTGTGTTTCCTTCTTCTAATTTAGTTTTCATTTTTTGAAACTCAGAAACCGTTCTTAATACTAAGTTTTTATCTATTCCAATTGTGATATAGAATTCTTTGCCTAATTCTTGTAGTAATTTAGGCGGAACAACAACTCTATTTTTATCATCAATTTGGCGATAATATTTACCAAACATTCCCACATCCTCCCACTATCAACTTAATTCTAACATATAAATCAAGTATTTTTTAATTTTTTATAAATAAAAATAAAAAAGAAGGCAAAAAAGCATTAGCCCTCTTGCAATAGACCATAGTAATTATTTTTTTAATTGTTTTATTAAAAAATAAATAACTGTATAAGTTTTTTGTTAAAATTAAAATATGCTAGTTTAGCTCAGTTGGTAGAGCAATTGATTCGTAATCAATGGGTCGGGGGTTCGATCCCTCTAACTAGCACCAAATGCACAAAGTAAGCACCAAAAATAAAAAATAAAAAATTTATTAAAAAATTAATTTTTTTTAGTTATAATAATTAAGCATTTAGCAAGTGCCGACTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTTGTCGTTGGTTCAATCCCAATAGTCGGCGCCATTATGGCCCTATGGTGAAGCGGTTAACACATATGGTTTTCATCCATACATTCGTGGGTTCAAGTCCCGCTAGGGTCACCATTGGAGAATTAGCTCAGCTGGGAGAGCATTGCCCTTACAAGGCAAGGGTCGTGGGTTCGAGCCCCTCATTCTCCACCATTTGTTTAAATACACCGCAAAAAAAGGCCAACCGGCCTTTTTATTTTTTATCTAGATTTAAATTAATCGTAATTTTTATATAAAAAACTCGTAACCTTTTTGATTACGAGTTTTTTATATCTAGTTTTTAATCATCAAATTGTGAGTAATATAACCTTGCATATTTTTTATTTAATTCTAGCAATTGCTTATGGGTTCCTTGTTCAATAATGCGACCATCATCCACAACTAAGATTAAATCAGCATTTTTAATCGTGCTTAGACGGTGGGCAATAACTATTGATGTTTTACCAACCATGATATTTTCTTTTAATGCTTGTTGAATTATTTTTTCTGTATTAGAATCTACGTTCGATGTTGCTTCATCTAATATTAAGATTTTTTTGTTTCCTAATATTGCTCTAGCTATTGAAAGAAGTTGCTTTTCTCCTTTAGATAATATTGAAGTGCTATTATCAATTATAGTATCATATCCATGTTCTAACTTTTCAATAATATGATGAGCAGATGCTAGTTTTGCTGCCCTTATTACATCTTCTTTGGTTGCTTTTGGATTTCCTACTAAAAGGTTATTTAAAATACTTTCATTAAACATAAAGCTATCTTGTAAAACAACTGCCGTAAAATCTCTTAAACTGTCTTTTGAAATTTCTCTTAGCTCTATTCCATCTATCTTTATTGAACCTTTTTCATAATCATAAAAACGATTCAATAAATTTATAATAGTAGTTTTACCTGCTCCAGTTTCTCCAACTAAAGCAATAGTTTGTCCTGCTTTTGCATTAAATGAAGCATCTGTTAGTTGATATTTAGGCAAATTTTCATTATATCTAAAATATACATGATCAAATGTTATATTGCCAACAGGATTTTCTATCACAATTCCCTTTGAGTGGTTTTTTATGTGTGTGTTTGGCAAACTTAAAAGCGTATTAACCCTAACAGCAGATGCAATACCAATTCTAGCATAAACTAATATAGTAAGCATTGATTGAAGTGTTGATAAAAAACTATATATAAGTGTTAAATAACTTATTAAAAATCCATGTTCTGCATTTGTATTAAAAAGTCCATAAACGTGAATATTATTTCCTTTTAATACAAGTGTTACAGTAACTATTAATATAACCAAAATATTGTTAGTAAAAACTATTCAAGGATTAATTATTTGGTTATTAAGATCTCCTGTGAAGGCTGTGTTAAATATGTTTCTTGCTATTTGTTTACCATGATTATTAACTTGTTCTTGTTGTTCAAAAGTTTTAATAATTTTAGAATTAGTTAGCATTTCTTCAACAAAGGCATTTAATTCCCCAAAATGTTTTCTGGTTTGAATAAATGCTTTTTGAGCTTTTTTAGTAATTAATATTCCTATAAATGAAAATAAGAATAGAACAGGTAAAACAACAGCAGATAATACTAAACTAACCATTGATAAAAATATTACAGATAAAATAACATTTATAGCACATTGGATTAATTCGTTTAATAATTTAAATATAGTTTCAGATAAACTATTAATATCATTTACTAAAATAGAAATAATATTACCTGATTTTTGATTATCAAAATACGAAACAGGAATATATAAAAGATTTTTCATGGCAGTTTTTCTTATATTCTCGCAAACTGAAAAAGTTATTTTTACAAAGAATACTCCTGAAACATATCTAAAAATTCCATACAATATAAACGATATAACCAAACATGAAGCCATACCTATAAATAATTTTAAATTAAAAGGTGTTTTATGATCTAATACATCTTTTGTTAAACATATTGTAAAAATATATCCAATTAAAAATGAACCTATTGCATAAAAAATTGCATGAAAAAATGAAAACAAAACAGCCAATCAAAATTTTGTTTTTGATTCACCTGAATATTTAAAAACTAATTTAAACAAAGTAATAAATGAAAGTTTTTGCTTTTCTTTAGTTCTTTTTTTCATCATTGCCTCCTTTTTTATTGACTTAATTGGTTATTATATGATTCATTATAATATGAACATGATTTCATTAATTCTTCATGATTTCCTTTATCCAACATATTTCCATCAGATAAAACAATAATTTCATCAGCATTTTTTATTAATGGCAATTTTTGGCTAATTATTATAGTTGAACATGAATATTTATTTTTTATATTTTGTAAAACTTGGTGTGCAGTAATATTATCTAAAGCACTAGTTGAGTTATCTAATATTAAAATTTTTGGTTTAGTTAATAAAGTTCTTGCAATTGAAAGACGTTGTTTTTGGCCACCTGAAAGGTTTGTTGCCCCTTGAGTAATGACATGATCTAAATTTTCTTCAAATTTATAAACAAAATCATATGCACAAGCGTCACGTAATGCTTGATTTATTTCTTCATCTGTTGCATCATTTTTAGCAAAAAGCATATTTTCTTTTATTGTTCCTGTATTTAATAAGGCATCTTGGTAAACAATTCCGATCGTTTCATGTAAATTTTTTGTATTAACTTCTTTAATTTCATTATCCCCAATTAAAATCGATCCTTCATTATATAGATAGTTATTAACCAATAAATTTGCAAGTATGCTCTTGCCACTTCCAGTAGGGCCAACTAATCCAAGAGTTTTTCCAAACGGTAGGCTAAAACTTATATTGTGTAAAGTATAATTTGGATTTGAAGAATAATATTTGAAGCTTAAATTTTCAACTTTTAAATCGAAGTTTTTTGTTATTTTTTTACCATCTGATACATAAATTTTATCTTCGTTAGTATTTAATACTTCTATAACTCTAGTAGCAGAAATTTTTCCTTTTACAAATGCTGAAATAAATACTGCCCCCAATATAACACCAAACGTTACATAAAATGAATAGTCAATAAAAATATTAAATCTTACAATTGTATCATATGTTGCTACATTAGAAATAACATCATGAACAACTAATGAATAAAGCACCACAACAAATAAATTAAATATTCCAAAGAATAAAGGAGCAATTCCTACAAAAATGCTACCAGCATTAACATTAAAATTCTTTCATGTTTTATTTATTTTTGCAAACTTTTCTTCTCTATTTTTATATAAATCATATATTTTTATCAAACGAGATCCCAATATATTTTCATCAACTTGCTTAGTTATTTCTTCAACGGCTAATTGAGCTTTTTTAATTGAAGGACTTGCTTTTTTGGCCATAATAGAAACTATTGTTAAAATTATCGGAACTAACAAAATTAATCCTATTGCCATTTTTACGTTATTCATGACGGCTAAAATACATCCACCAATTATCATTGAAATAGCCCTTGTTAAGACATTTGTGGCACTTATTAAAAATTCTCAGAAAACCGCAATATCATCACTCACCCTAGTAATTAATGATTCGGGTTTCAAATCAGATATATTTTTCAAACTTAATGTATTTAATTTTCTAATAAAGTTTTCTCTAATATATCTACTACTTCTTTCTGCTGCATATACAATTAAACTAGTGCAGCCAAATGTTAAAGACATTGAAATTATAGTTAAAACTATTATAGAATAAATCATATAATTGCGAGCATTAATAGCATTATTAAATGTAAAGTTTATTAATCCATTAAAAAAACTTAAATCTACTTGGCTATTTTTATTTTCAATAAAAAGCAATCTAATGAATTGAGAAATCAATGTAGGAGTAATCAACTCAAATATAACTGAAATTAAAGAAATAAAGCTTCCTACAATAAACAATATTTTAATTTTTCATGGTAAAAATTTAATAAGCTTCAACATAATTTTTTCCTTCTTTTTAAATGTTTTTTATTGCCACTTTCAAGACTAATTAATAAATTATAATGAAACATAAAAAAAAGTAATAATTTAGTGCAAAAAAATACGAAAAAAAAGCGATTTTTCGCTTTTTTTGTTTAATTTATTTTTTTAAACTAATTTATTTATAAATTCAACTTCTATTCCATTTCATTTAGCAGTGATTGTATATTTTATATATTCCTTCTTATTTTTGGTTGCTTTTTTGAATGAAATTTTTTCAAATGTTAATCCTTCTGGTAATTTGCATTCGAATTTAGAAATATCATCTTTAATTACTTCATCAACTGTTTTTTTATCGCCAATGTATTTTACGGTTACTAATTCTCTAAAATGTTCTTTTAAAACTATTTTTTGCATAAAGTCTTTATCTTCATTTATCTTTTCATTAACATTGACACGTTTAGATGACATATTGCTAAAATCATTATTTTGAAAATCTTGAATAGAAACTATAGAAACTCTAATTGATTTTACTTTATTATTTTCTATTTTAGGAAATGAAAAGATATAAACATTTTGTAAATTAACCATATGTTTTGTATATTCAAACGCAAACATTGAATGTTTTAATTTTAATGTTGTTTTATCATAATCAAAATTTTGAACCGAAATGTAATTATCACCTTTTTCAATCATTTCTTTTTCAATGTCTTTGCTTAAAAGACTAATATTTTTCAATTTTTCTACAAACATAAATGTTGAATTATTTTTAACTACATCATTAGATAATTTATCTTTGTTTTCTTTGAAATATTTCAAGAATGCATCTTTATTTAATTCTTTTACATCTTGGTCTTCAATAGTTGATGTTGGGGCTTGTTGGTCATCTATTTCAGTTTCATTATTTTGTTTTCTAATCTTGTTATATTCATCAACGCCCAATTTACTTAAATCAATTTCAACAAATTTTGATTTCTTAATACTATTTTCATAATCTTTAAATGAAACAGTAAGATTAATCAAACACTTATCGCCTTCAAATTTTAATTCACCTAATGTGTAATTAATTTCACTGTTTTTATTATTAATTCAGTATGATTTATTTTCTAATTTGTCAATAGATTTCTTTGAAATGTAATATTTGTCTTTACTATAAAATGGATAAATTGAAGTTTCTAAAGCTTTGTTTAAAAGTTCAGCTTGTTTTTCTGGTGATAGCAATGCAATATTATCTAAACCATTTGCAAATTTATCTAATTTAACTTCAATAGTTTTAGATTCACTTATTTCTTTATTTAAAAGACTTGACAAAGTTAAAGTCAATGTTATTTTTTTATCTTCAACTTTTAAATTGCTTAATACTACTGAATATGAAGCTTGATCATAGCCTTGAACTGCAAAATTTGGTTTTTTATCAAAATTATTTATTTGTTTATTTTTTAAAATATCTTGATATGACTTGGCATTTGCAACTAAAACATTTGTTTTTTCAATTTGATCTTTTAGTTCTTTTCTTGCCTTATCTATTTTTTCTTGCAATTCTGGAGTTAATTTAAAACCTTTGATTTCATAAATATATTTTTGACTTGAGATTTGTTTATTTTTATATGATAAATAGTAAAGAATTGACAAGTCATATGTTCCATCTTCTCCTGTATTTTTAGTAATCTTTAATGAATCTTTAACAATTGAAAAGTTTTCTTTAATAGTTTTTAAAAATACTTTTGATGTGTCCGCGTCTTTTATGTATGTTTCTTGTTTGTTAGGATAGTCAAAGACTAGATTTTTTGAAACTTCTTTAAAATCTTCTTCTGAAACTTGATCTAATCCAGAATCTAAGTTTCCCAAATTACTTTCATAAACCTTGTTTGAAATAATATGTTTACCATTTTGTTTAAAAATTGCAACTTTATATTTCAAAATAAAGTTTCCTTTTTCATCTTTTGTTAATAACAATTTAGAGTTTAACTTGTCTCCACTTCTAAGAGGTTTTTCAGGATTTACCAATTGAACAGATGTGCCTGATTTAGAATTTCATTTAGCAATAGCAAATTCTTGATTTTTAAACTTATATTCTCCTTCAAAAGCCATGATATTTTTGTTATTTCTTTCAAAAAGAATTTTGTATTTGTCTAGATTATTTAAAACCTTTTTAATAAATTCTAGATTTTCAGCATTTCATGTTATTTTTGAAATTGATTCTTCGCCATATTTATCAGCATCATTTTTATTTGTTTCTTCTTCATGACTTGGATCTTCTGGTTTAACTTCAGGTGTTGTTCCTGGGTTTTCGCCTTCTGGTTTTTTAGGTTCTTCTGGTTTAACTTCAGGTTTTTTTGTTTTTACGCAACCTGCTGCCACTAATGGTATTGCAAAAACCGAGGTTGCAACACTACCTAAGGCTAATAAAATTTTTGTATGTTTTTTCATTTTTTATATTTCTTTTCCCTTTTTCTTAAATTAATAATTTAAAAATTTATCACACGAATAAAACCTCAAGTTCAAATGACTGGTTTTTTCTTTAAAAAAGAAAAATAAATAACCGTTTGGTTATTTTTTATTTAAAGATAATGTTTTTAAAAGAAACAAAAAAATAATAAATTACAAAAAATGTTACGTTATTTTTTGATTTAGCAAATATTATAATGTGTTTTTTTAGCCTATAAAAATATTTAAAAAACTAGCATTTTAAGCTAGTTTTTTAATATGGTACATCCTGTGGGGCTCGAACCCACGACCCAGGGATTAAGAGTCCCTTGCTCTGCCAACTGAGCTAAGGATGCGTGTTTTTGCGTTATTTATTATAACATAACTAAAACATTTTTTTAAAATTAAACAAATAAAAAAGTAGCGTTTGCTACTTTTTTGTTACAACTTGATGATATGGTACGCGAGGAGGGACTTGAACCCTCACGCCGCAAAGCATTAGTGCCTAAAACTAACGTGTCTGCCAATTTCACCACTCGCGCATATGGTGCCGTTTATAGGACTTGAACCTACGACCTACTGATTACAAGTCAGTTGCTCTGCCAACTGAGCTAAAACGGCTTTTGTAAATGCTTTATTATTATATAAGGAAATTTTAAAAATTTTTACATTTTTATTCAAATTTAAAATTTTAATTATTCATAATATCTTAAAATAAATGTTGAAATATTATTTTTTACTATTACTATATGATTTTTTTATTAACTTTAATTTTTAAAGATAATAAATTAATATTTTTAATGTATGATTACATGGCAAGGACAAAATTATGTTTTATAAGTTATATAAAAAAAGAGGCCAATCAAGTTTTTTTGCAATAAAAAAATTTGCAAAAAATAATAATATCAATAAAGTTGGACATTGTGGAACATTAGATCCCGAGGCTGAAGGTCTTTTAATCGTAGCAACAGATGAAGATACAAAGGCCTTGTCTTTAATTGAAAATCAAAACAAGGAATACTATGTGGAAGCGAAATTCCATTACTCTTCAGATAGTTTTGATTTAGGAACATATGTAACTAAAATACAAAATTTTCAAATAATAACTAAATCTCAAATTATACAAGCACTAGAAATATTAAAAAAACAAACAAAACAAGTACCTCCAATTTATAGTGCAAAAAAAATTAATGGAACAAGAGCATACAAACTTGCTAGAGATAATAAAGAATTTAGTTTAAAAGCCAATGATATAAAAATAAATAATTTAGAATTAATTGAATTTAATGAAGTGAATCAATCAATCATCATTAAGGTCAATGTTTCAAAAGGCACATATATTAGATCATTGATTCATGATTTAGCTAATATATGTAACACAGATGCAGTAGTAGTTGTTTTAAAGCGAATTAAAATTGGCAATATATGTTTAAATGGTCAAGAAGAATTTGAAAAAATTACAAACTTAAACGAACTTTTTAATGTAAAATTATTTAAGCTAACCAAAAATGATATTCAAATATTAGCAAACCAAAATTCAAATAGTTGCTACTTTGAATCATTGAAAAATTTTTGCGGAAACATTATTTTTATTTACTTGAATAACATAGTAGGATGAGGAAAATCAGAATTTGGAAATGTAATTATTTTCAAGCGATTTTTTAAACGCCTTACGATGTTAATTTCTAGCTAGGAGTAAACATGACAAAATACAAAATGATAGTATTTGATATTGATGGAACATTGTTGCCGTTTGGAGTTACTCAATTAACACCTAGAATGCAAGAAATGTTTGAAAAATTAAAAGAACAAGGCTATGTAAATGTGTTGTGTTCTGGTCGTGATATTTTTACAGTAGGCGCCCAAATAGATAACCCTTATGTTGATTATTTTATAGGTGCAAATGGAACTTTTATTCTTGATTTAAGAACAAAAGAATATATTTTTGAAAAAACTATTAATTACAAAGATTTTGTTGAATTTAGAAAATATGCAAGCGAACATAAATTATCTTTTTCTTTTGTTGGAAACAAATGGGGATATTACAATGAATTATTTAATATAGATCATTGATTTTACAGACCATATAAAGACAAATTTATTCCAGAAAAAGAATTCGAATCGCAACATGATAAAAATTATTTAATTACTATTTCAACTCCAACACCATCTATAGTAGCAGCTGATTTAGAAAAATTCTTCAAAGAAAAAAATCTTGATTTGTGAATTCTAGCTCAATGAAATGGTGGCATATTCATATCTGCTAAAGGAATAACTAAGGCTGTAGGTTTAAAAATACTAGGAGATTTATTAAATATTTCTCTTGATGAAATGGTGGCATTTGGTGATTCAGAAAATGATATTGAAATGCTTGAAAAAGTAGGATTAGGTATTGCTATGGGCAATGGAGAAGATGTTACTAAAGATGCTGCAAATGAAATTTGCTTGCCAGTTACTGAAGATGGTCCATATTGAAAATTAAGAGATAAAGGATTTTTTGACTAATAAATTTCAACCTTATGGTTGATTTTTATTTATTTTATTTTAAAATACTTATATTAGGAAAAAAACACATGGCAATTACAAGTACACAAGATCCAAAAATTCTAAAATATAAAAAATTACTTACTCCCAAAGGCAGACAAGAACAACAATTGTTTATTGTTGAAGGTTATCATTTAGTAAAAGAAGCAATCAAGGCAAATATAGTTATTGACATTTTAGAATTCAATGACATACTAACTTATAAAAACTCAACTCAAGTAACTAGAGCAATTATTAAAACATTATCAACAACGCAAACACCTCAAAATATCATTGCAATTTGTAAAATACAAAATTTAAATATTAAAGCAAATAAAATAATAGCCCTTAATAAATTACAAGATCCCGGAAATATTGGTACTATTTTTCGACTTGCTAAATCTTTTGGCTTTGATAGTGTTATAGTTGAAAATATAGATATATACAATGAAAAGATAATTCGTTCATCACAAGGAGCAATGTTTTCACTAAACATTATTAAGACAAATGATTTAAGCAAAGAACTGTCAGCCTTTAAAAAAGATGATTTTTTAGTTTATAGTACAACATTAAATAAAAATTCAATTAAGCTCAATGACATTGATTTTAGAAATAAAAAAATTATTGTTGTTTTGGGAAATGAAGGAAATGGTATAGACAAAAAAATTATTGACTTAAGTGATTTTAATGTTTATATTCCTATTGAATTTGAAAGTCTCAATGTTGCTTGCTGCGGCGCAATTATTATGAATAAAATTTATAATGGAGATAAATAATGCAAGTTTTTGATTGAGATTTTAAAGAAAAATTAAACATAAATGAACCTTTAACAATTTGTTTAGGTTCGTTTGAAACATTACATTTAGGGCACAATGAATTATTTAAAATAGCAAAAGATTTAAAGCAAAGTTATCCAGATCAAAAATTAGCAATAATGATGTTTAAAAATCCAGTTAAAAATTCTCAGGTATTAACAAAAAAAGCAATGCAACCAAAAACCAGATTATATACATTAGCAATGTTGGGATTTGATTATGTTTTTTTAGTTGATGTAAACCAAACGATATTGAATTGTAGTTATAGCGATTTTATTCAAGGATTAAAGATAAATAATGTTAAAAATGTTGTTTGTGGTCAAGATTTTAGGTTTGGGTTCAATAGAGAGGGTAACATTGATAAATTAAAAAAATATTTCAATGTATATGTTGCCAATGAATTAAAGGTAAATAAACAAAAAATTTCTTCAACAATAATCAATGAATTTATTGAAGAAGGAAACATAAATGGCTTGAACAAATTACTAATAGACAAATATTCAATAATTGTAAATTCTCAAAAATTTAATTTCAAATATCCAGAACATTTAAATCAATTAAAAGCAGGAATTTATATTGCCAATGTCGTTTGAAATAACATTGAATATCACGGCTTAATTTTTATAAATAAAAATCAATTTGACTCAAATAATAACCTACAAGAAAACAACATAATTTATTTATTTGATATTGATTATTTTATTTCGAAATATCAAGAACTTTACATTGAATTTTTGCAATGCATTAGACATATAAATAATAATAGTGAAAATCAAATTTCAACAAATGATATTGAAATTGCAAAGAATTACTTCATTGCCAACAAAAAATAATATTAAACAAAAATAGCAAGTTTGTAACACTTGCTATTTTTTAACAAAATATATTATTTTAATTTAATCTTTGTTGTTCAAGTCAATATATTCAATATCGCCGTTTGAAATAACTTTAATTAACCCTAAATTATTTAAATATCCATATAGATCATTTAATTTTGAAATATTATTTTCTGTTCTTGCTATTAGTAATGTTTTTAAAATAGTATTAGTTAAATTGTTTTTTAAAATAGAACTTTTTGAATTGTTTAAAACTTGTTTTATTAAATCAAACATTTCCAACGAACTTATTTCTGATATTTTTCTATTGTCACAATCCTTTGGCATTCTAAAAGTATTTGGCTTTGAAACATCATGATAAAAGATTTCGCCATCAGGATTTATTAATTTTTCTAACATTTTATTATTAACAAAATAATTTATAGAATTTTCACAAAGAAATTTATAAGTATATTCGTCTTTATTTAAAGCTAGGTTCACGAATATATCTTTTATCATTTTATTGATTTCTTTTTCTCTATAAGTATGGCCATAATTAAACATTTGTTTAACAATTTCATAGCCATTTAACAAAGGATTTCTTTTGTTTGACTTTTCTATAAAATTTAGTGTCTTACGATATGAGTAATTAAACGGAAGTCATTCTTCAAAATTAAAATTATCTCTTTTTTCAATAATTTCAAATTGACTATTTTCAATCGTTTTATATTCATTTTGCTTTACAACAAATTTTTTTTGTTTAATTTGATTTACTTTATCAACTATTTTTTCTAATTCTCTTTTTTTGTTTTTAAATCAATTTAAAGATCAAATTCTGTGAATTATTCAACCTCTTGACTCAAGTAGCATTTGTCTAATCGCATCTCTATCACGAGCGCTTTTTGATGAATGATATGTGGCGCCATCGCATTCAATACCTAAAATATATTTCGATGGATTTTTATCATCAACAATTGCTAAATCTATTTTAAATTTTGAACATCCCACTTGAGTATTTACTTTAAATCCAAGCTTCTTAAGTTCATCATAAACTTGGATTTCAAACTCACTATCAAATATTTTAGTGTCATCTTGTAATATATCATCTGATTTGCTGTCGTAACCAAATTCTGCAAATTGTAAATATTGCTTTAAAAATCTTAGTCCTTCGTTTTTTAAATCGTCTACTTTTAAATCAGAATATGATATTGAAGATACAACAATAGTTGCTTCCTTGGCTCTACTTATTGCGACGTTTAGTCTCTTTTCACCGCCAACATTATTTAATGGTCCAAAGAATCTTGACAATCTTCCGTTTTTATCCCAACTATTTGTAATTCCTAAAATAATGATGTCTCTTTCATCACCTTGAACACTTTCAATGTTCTTAACAAAAATTTTATTTTCTGTGTCTAAATCCATATATTCAGGATTTTCATTCATAAATAAGAATAATTTGTGATCAATTAAGTTAGCTTGTTTTAAATTAAACGTTACAACTCCAAATGTTTTTGTATTGCCATAATAATCTTTTAATTGCTTTATTAATTCAATGATTCTATTTGCTTCAATTTCGTTTGTTTGATCTTCATAAATTCCATTAACCTTAATAAATGAAACGCCTTCAAAAGGAAGCAATTCCCCGTCTTTCTTGGCGGTTTTTTGATATGTTGGAAAAGTAATTAATTTATTATCATAAACGAACTTATTTGAAGGCAAAATTAATTCTTCAAATTTTGAACGATAGTGTCATTTTAATGTAACTTGTCTAAATGAGCTTTGCATTCTTTCAAGAAGTGAATCATAATCATTTTCTTCGTTGACTATTTCTTCGTCTTCGTTTGGTTCTTGCTCGCTAGACATAGTTTTAAAGAAATTAGTTGGTGGCAATTGATTTATATCACCAGCAATAATAATTTGTTTAGCCTTAATCATTGATATCAAGGCATTTTCTGTCGTTAATTGAGAAGCCTCATCAAAAATTACACTATCAAATGTGATGCTACTATCCGCTAAATATGTACTTAGCGAAGAGGGAGACATCATAAAACAAGGCTTAATATTTTGAATTAAATTTGGAATTTCTTTAAATAGTTTTCTAATCGGTTTTTTATTTCTAACTTTTGAATATTCTTTCTTTAAAATTTCTAATTCATCATTGTATATTGTCGAATTTAACGTAGGCAAATTGTTTGATAAATTTTCAATTATTTTGAATTTAGCAATTTCATTTAGCTTTAAACAAGAATCTTTAAAAACATTGATAATTTTTTCATATTCTCTATCGTTTCAATCTACTAAATCAATTGATAAATAGTAGTCAATTAAAAGTTGATAAAATCTTTTATTAAAAATATTTTCAATACTATTTTGCTTTAAACAATCAATATCTAGCAATTCAATAGCATCCACAAAATCATTAGTAAAGGGATTGTTTTTTAATTCTTGTCTTGATTTTATATAACTACAATATGCTTCAGGATATTCATCAGAATCAATTAAAAAATTTAGTTTTTTTATAAAATCATTAACTTTATAATTTGAATTTGAATAATCTACATATTTATTGTTAAAGTAGTCATTGAATTTATCTTCAAAAGTCATTGAATTTTTATATAAATTTATGTAATTATTTGTTAATTGAATATGGCTTAATAAAAAGTTAAAAAAGCTAATTTTTAAATTTTGATCAAATTTTAATTCACTTAATTGATTATTAATAATTTCGTATTTAGAAAATGTATTTAGTTGTTGCTTTAAACTATTAAGATCCATTTCATTATAGTTTTCTTTAAATGAAAAAGGAATTTTATTTGTTGAAAGCGTAATTGCATTTTTAGCAAGTTGAATGTTGTTTAATGCCACTAAATCTTTTAACAAGTTTTTGTAATTTAAATTAGAAGATATAAAATTAATTTTTAATTCTTTTTTTATTGCTCTATATCTCTTTGAAAAAAGACGCTTAAAAATATTATTTCGAGATTTCACAAAATCTAAATTCTTATAAATTTCACTATTAAAAATATTTTGATTATATCTTGAAAGAATTCAGTCAATATCAGGTTGAATTCTTTCTTTTTGCAATAGTATTTCATTTAGGGTTTGTGTTGCATTTGTTTCATAATACGAAAAAATTTCTAAATATTTTGGATTTATATCTATATCTTGTATATTTGAAAAAATTTTAAAAAATGAATTAAATAATGAATAATTGCTATTTTTAATATATGGTTGATAATTGTTTGGAATTTCAAGATTTAAGAAATTGATTAGGCTTTGAATTTCATTAATTAAATGGCTAGAAATCTCTTTAATGTTTTCTAAATCTTGAAGCTTTACATCCAAAATCGAAAGCCCGTATCATAAACTTTCCTTAGCGTTAAAATTTATATTTTTATAGTTTAAATTAAACTTATTTAAACTACTTTGAATTTCAACAAACTGTGCATTATCAATTTTTTCAAAATCTTTAATATCAAAATTTATACCTTTTGTATTGGCAAGTAATTTTTGATAATCACCAATCATTAAATATAATGAGCGACCTAGTGGTTTTCTATTTGATAATATCTTTCTAACAAAATCATTCATTTTTTCTAATGAATCACTATATAAATAATCATTATTAGCAATAACTTCATCACTTACTTTTTTAAATTTTTGACCGTCTTGCAATGTTTCATACAAATCATTAGAAAAATCTTTTTTATTGCTGTTATTGCTATGAATAAGTAAAATAAAATTAGCAAATCCTAAATTTTGGATTGATTTATAAACAACATCCAAAGCAGCCTTTTTTTCAGCAACAAATAGAACCTTTTTATTTCTTGCAATTAATTCACTTATTATATTTGCTATAGTTTGAGACTTTCCAGTTCCTGGAGGACCTTGAATAATAAAACTATTTCCTTTAATTGCTGATTGAATAGCTGCTTCTTGCGAAGAATTAGTCATTAGGCAGTGATAATAGCCTTTAGGAGAAACTATATTATCAACTTCTTCTTCAGAATACAAATATTCAGAACTAAAAACATTGTTAATCCCCGTCATTGCATTCATAATTTCTGAATTAATGATATTATCTTTGTTTTTATCAACATCTAAATAAATGTCCATAGAAGAAAACCTAAAAGCAGACAATGAAGCTTCATCAATTATTTTTCAATCTTGTTCTATAGAATCATTGAACGAATTTATTTTTTGATTTATAAACTCAATGTATTGTGAATATCTATCATATAGAGAACCTTCGGCATTATCAAATGCTTCAAATTTAATATCAAGTCCAAAATCATCATTTAATTTCTTTATCAATGAATTATTTACAACAAAGTCATTGCTTTGAATAATTATTTTTTTATTTTGCAATCCATTTTCGCTTTCAATTGAAACAGGTAAAAACAAAAGTGGGCTATAAACGTTTTTTTCTGTTTTATCCTTTCAATGAAGCCCACCAAAAGCCAAATATAGAATATTAATTCCTTGTTCTTCTAATAAAGTCTTTTGCTTTCTTTGCAATAACGACAATATTGCTAATAATTCTTTTTGAGAAGCAGAGGACAAAAATTCATTGCTTTCAACTTCAATGTCATCATCAGAATCTTGAGAACCAATAAATTTAAATTCACTTCTATTTTCAATTTTATCTAAAATTGATTTTATGTCTTCCTTTTCAATTGTTATAGTTGATGATTTATTTTTTTTATAATTTATCAATATATTTCTTAATGTTGTGTCTAATAGCTTATTCTTTAAAGACTCATATGCCAAAGTTTTTGTAATTAATTCTTTATTTTCCATTTCTAAATTATATTATTTTAATTTTAAAATAAATGTTGTTTAATATAAGATTTTATTAGCATATTTTAAAAAACAAAAATTTTAACGCTTGATGAATTAAAAATTGAAATTTTTACATTTATTGATCGGCATAATAATAAATTCATTAAAAAAATGATTCAAAAACACCCCAACAAATGTGGGATGTTTGTAAAAAAAACAAGACTCGTTTTATTTTGATGTGAGTCTTGTTTTTTACTTCTAGTTGCTTTTTTCTATTATTGAACCCATTTCTAAAAAACCTTGAGCATCTAATGCTGCTCCGCCAACTAAAAAACCATCCAAATCCTTAATTTCAAGCAATTTTTCAATATTTTTTTGTGAAACTGAACCACCATACAACACTGGAACTTGATTATTAGTAATTTTTTTAATTACAGATATTGCTTTTTCGATATGGTTATTTTGAGGAATTTTTCCATTTCCAATGCAATAAGTAGGTTCGTATGAAATAATTAAACGATTATAAACATCGACGTCTTCTAAAGCTTTTAATATTTGGTTTTTTATAATATCTTCAAAGACTTTATCGCTACAATTTTCTTGATCTTCCCCTATACACAAAATAGGAATAATGCTAGTTTTTTCAATTTGTTTAATTTTTAAATTAATCAATTGATCAGTTTCATTAAATAATTTTCTACGTTCACTATGTCCTATTAAAACAAAGTCAATGTTCAATTCAACTGCTGATCTAAATGAAACTTCCCCAGTGTATGCACCTTTTTCTTTATAAAAAATATTTTGAACTCCAAACAAGAAATTTCTTTTATTATATTGTAAAGACAGAGACATTGCATCGAATGACAAAGCCGCTCCAACTATTACATTTTTAAAATTTTTAATTTTATTTAATGATTGTAGTTCATTTAAATAATTTTGACTTTCTAAATAAGATAGATTCATTTTTAAATTACCAATTAAATATTTCATATTAACCTCTTTTATTATCAAACTATTTTTAATGCTAAAAATGGAATCAATATAATTAGTATAATTAATCCATATAATATTAATAAAAGAAATGATAAATTATTAATTGATTGATAGTTTCTTTGCTTTTCAGCTTTGAGTCTTTCGCGTTCAAATTCTTGGCGTTCTTTAGCAATAATAACATCCTTTTGATGAATTGTCATTCCTGAATAAAATTTGTCTTTTGCGCGACGCATTATGATTCTATTTCTAATTAAATTAGCAATAGGATTGACAATTCATTTTCCCATTCCTTTTCTATATACAACAATTATAAAATTTATAGTAATTGCAAAAATCATTGATACAGTCAATGAATTTTGCCAAGAATGAACATATTGACCATCAACAGATTTTTTTGAATATCTAACTATAAAAATTAATGCAATATAAATCACTAATGAAACAAAAAAATAACTAATCGTTGCTTTATTTCAAGCATTCTTTCAATATGATATTGATTTCTTTTGATTTCTAAAATTTTTTTGTTTTAATGTCATAACATTATTTTATATTATTTTTTAACAATGCAAAACAAATTTAAAATATATCTGATTAATGATTTAAAAAATAAAAATTAAATATTATTTTGTTTCTTTGCATACAAGGTAAATTAATTTATTTGATTAGTGAATTTATTTTTTTGTGATAAATTTCGTTAAATTCTCTTTTATCATGCTCAACTTGTTCGAAATTTGTATATGAACTGTCTTTTTCGTTATTAACTAATTTATTAATATATGGGTTTATAAATTTTTCAATAAAATCTTGGCCATCTGGTTTTTGAAATATTTTATTTGAATTAATTTCTGCATCAGTCAAAGTTTCTACAAACTCTCAAATAGACTCGTTTAATGCTAACTTTTGACTTATAGAATTAATTTGTTCATTTAAAGATTCTGTTAAATTATCATTTGCATGACATTTGTTAATTTTTTCAATAATTTGCCCCTTAAAATATTGATATTGATTTATTAATGGTATATATTTTTCTTTTAAATTGTTTTTAGCAATTTTATTGATAATATTTTCAATTACTTGGTCTTGGAATTTTTGTTGCAATGCTAAATTAATAAATTTAGTTTTTTGAATTCTAAGCGAGGTTATTTGCTTAATCTCGTTCAATTTATTTAAATAACTGGCAATATCTTTGCTATTAATATTATTCAATAATGTTTCTAGATTAATTGCCTTTAAAGTTAAAGATAAATTATCAGCAATTGATTTTAATTCGTTTCTTACTAATTTATCTATTTTAGAATTGTCTGCATCTAATTTAATTTTATTTTCAAGATTATTTAATTCGTTTACTAAATTATCAATTTTTGTTAAGTCTTTTAAAAGTGGTTGAATTTTAAGTATTTTTTGTTTAGCAACGTTTATTTTTTGTATTTTTTCATTTAAACTTTCTATTGATGATTCAGGGGATTCTTTATTAAAAGATTCCAATAATTTCTTGGCATCTTGAACTTCTTTTAAGGTGTTAAATTGTTCAGCTCCTTTTACCAAAAAGTTATCTTTTAAATAACTAAAAAGATCAGTTTTTGCATCATTAAATTTTTTTAATAATTCTTCAGATTTTTGTGCATTTTCATCTTTAGTTTGTGTGGTTTCTATATTTTGTTTAGGTTTTTTAGAACAAGAAGCTGCAACTAATGGGGTTATTAAAATAGTTGAAGATAACGGTAATATATAAAGTAATTTTTTTCTCATTTTTCTCCTATGAAGTGTGTAATATTTTTTTAATTTTATATTCTTAAATTGTGCTATCAACTTGTATTTGTTATAAATCACGATTATTTTACCAAAATGTTCAATAAATTTAAGTAGTTTTATTAAAACAAATTATTTTTATAAACCTAAATAATCAAAAAAATAGTCTAATTTAGGCTATTTTTTTGATTAAAATTCTTCATTATTTTATATTAATCAATTTCAAGTGGTACTTCGCTATCACCGTCACTACTAAAAATTCCATTATTTGATTTATCAGGGCATATTACACTATAAGAAATTACTGGCAAATCCCTTAATCTTCCCTTTGATTTTAATTGTATTGAAATGGGGTGGCCAAACGGAATAAGTATCTTAGGCAATGTAACCTTAAAATATTTTACAAAATCTGCATCATTTATTACATTTTTATATGCTTTATAAACTTGCGAATTTATTCCACTAGTTTGTTTATTTCTTATGTTGTTTTCAATTTGTTCATCAATAGATTTATACTTATTGATTATTCCTTCTAAAATTCTTTGTAATTCTTTTGTTCCACGTGCAGGGTCATTTAATATTTCTTTACCTTCATTAGTTAGAGTAAAACCAAATTGGTCTCTAATATATGACAATTGGCTTTGGCCTAATTGACCTTTTGCTCTCTTATTAGGATCTATAATGTCGCCAGATTGAGATTGTCCACTCGAATCTTCACCTGTGGCTGTAGAATTCTGACTATTATCTGTTGTTTTGCATCCTGCTGCAACTAATGGCATTGCAACAACTGAAGTTGCAACGGCACTTAGTATTAATAAGCGAATTTTATTTCTCATATTATTCCCCCTGAATATAAATATATCGTTCATCTTCAATAACTTGTAAAAATTGGCTACTTCTTAATTTACATATTATACTATATTTTATAAATATAGTATCTGATTTTAAATTTGTATCACCATATCCAAGTTCTAATTTTAATGTATGATTTAATAAGTATTTCGAATCAGGCAATGAAATTTTAAAATATTTTAAAAATAACTCATCACTGAATATGTTTGACTTTTGATCATCTTTTTTGTATTTATCATTGAGTTTATCAATGATTTTGAATAAAAATTTCTTATTCTCTTGATTAAGCGGTATTTTAGATTCAAAATTAAAACTATCATAAATTGCTGCAAGCATAGTACTAGACATGTTTTGATTATTATTAGATAAAACATTATATTTAGTATGACATGGGCAATTAATTCCTTCAATAATCTTGGTTTTTAATAAATCATTTTTGGTTTTTAAATTAGAGGATTTTTGGCATGAAACTGCTGCAATTGTTGGAATTATTAAAACTATTGGTAAACAAAATAGTGTAATCTTTTTATTCATTATTCTAAATCTAATTTTATTTCTTGATCTTTGGCATCATATGTTTTTACATCTTTGCAATATATGTCATAGATTAATGAAATATTGTTATTATTATCAATTTTAAAATTAATTATCAATTGGTGATTTACACCTAAATTCGGCTTAACCACTTTAAACAATTTTAAAAAGTTTGCATTATTAATTAACCTCAAACCTGCTAATTTATTATTTTCAGAAGGGCTAAGATTAATTTGCAATTCATAATCTTTACAAATCTTTTTTAGCTCATCTGCCAACGCCTTATTGCCATAATTATTTTTTAGTATTTTGCTCTGTTCAGTTAGTTCGAAAACAAAATCATTTTTGATTGCTTCTAATTGAGAAGAATTAAAAATTCCACTTTTATTAGTGTAAATTTCATTTGCTTTTGCTGTATTTTCATCTGTTGAAACACACGATGCTGAAATTATTGGCGAAGCTAAAACTAGAACTGCCCCTATAGATAATATTATTTTTTTCATATGTTTTTCCTATTCTATTACAATATCTTGCAAGTAAGACAACCCGTCATAACTAATTGGTTTATTAATAAATTTTTGGTATATTACTTTTGAAAAACCAACAGTTTTAATGATATTTTCAAATGAAAAGGTATAAGAAAGTAAACTGTTAATATCATTAATTATATTTAATTGGTCTTGGTAAGAGATATTTTCTAAGTATTTTAATATAATTGATTTTATTATAGAGAAATTTTTGTTGTAATCTTCTAAATTTTTGTTAAAAATTTCATTATTTGTAATTAGTTTTGTTTTATTTAATTCATTTAATATTTTTTTGGTATTTTCTAGAATAAAACTATTGCTTTTAGTTAAGCTAGAAACTTTATTAAAGATTAAAAACAATTTATAGTTTTTAGCAATGGCTGTTGCTATAAATTCTTTAGTATTTGATTTTAAAAGTTCAAAGCTATTATTGCAATATATTGAAGCATCTTGATTAAAATACAAGTCATATTTTGCTAAATCATCAATAACTACAAAATTTGGCTTTAAATGTTTATTTACTGCTTTAAATATTGTTTTGATATGTTCCAAAACATTTTGCCTTTGGTTATTAAATTGCAAATTCAAAAAATTTAGTGGAATATCAAATTCAATATCAGCAGCATCTGGATGTTGTTCATAAAACTTTTTGATTATATCATTTAGTTGTTTAGATATTTCTTGAAAATAAGCTGATTTTAATTTGTAATCAAGATTGCTTGATTGAAGATCGTTTAATATTTTTTTGTTTGCAAGATAATAACTATTGTCAATAAAGTCAAGACTATTTTCATGAACATTTTTTGGAATCAATGGCTTAAAAATGTTTACCATAGAATCTTTTAAAACATGATAATTAATTCCACTATCAAAGCTTGAAATATCTAAATCGCTTGGCGCTTGAGATGTTCAAATATCACCTTGGTTAGCCAACATCCCATATTGATTAATCAATTGGTGAATCAATAATAAAAAGGCAATATTTTTAGATTTTAATATCTTATTTATATTCGTATTTTCATTTAATTGGTTTGCTTTTAAACCATATGCTAGCATTGAAAAACTATCATTAAAAAATTTTTTATTTGCAGTTGGCATTCAATTAATAGTAATATTATTACTCAACTTATTTTTAGAAACATTGCGAGTATAGCTTATATTAAAATCATTATATTTATGCAAAATTTCTTGTTGTTGATTTATGTCAAGATCGTCAAAATTCAATGTAGTTACAATATTTTGCAAAAATGCATGATACATTTGCATGCCAAAAGTATTTTTTGATATTGGCAATGTATTAAATTGAAAATCAATTTTTGAAATTGATTTTTTATTTTTATCGTAAAAGAAATTCTTTAATAATTTTATTTCATCTACATTTACTTCATTAATAAAATAATAAGATGCATAATATCTATTTTTAAAAGTCTTATCATATTCATAAAATCAGTCAAGATTTGTTAAAAAATCTTTATATTTTTCATTAATGATTTCATAAGGCATTGCATCAATGTAATTTGAATGTATAAACAAATTGTTAAATAAATCAACTCAATCATGTTCAATATTATCAGCACTACAAATACTTAAATTTTTGTCATTAAAGTCTAGTAATTTATAAACATCAACGTTGTAACTTTTTAAATATTCAAACAAATCAAAATTATTCAATTGATTTTGCTTTAAAAAATCTTGTTCTTTATCTTTATTAAGTGTTATATTATTTTTTGATGCTCATGATCTTCTAAAATTTTTATTTAAAAGTAGCATTCTTAAAAGACCTAATTTGAAATCTTTTACATTTACTCAATGAACTTTATTGTTTATATTTCTAATTCCTTGCCAATTAATTCAATAATTTTTTCTTTGATTTATACTGATTGAAATATTTTTTGCTTTTCTTAGAGATTCAATGAATTGTTTGCTATTTATAGACATTGAATTTTTTGAAGGAACGAACAAATATGGATTATTAAAACCATTGCCCTTGTCTTTGCTGGGGTAATATTTGTTGGGATCTTGCTTATTCAATTCAGTGTTTTGATAGTCAACATTTAGTTTATCATCGCTATTGAAAAGTTTATCATTTATTTGAATACTATTACCATATTCAAATTTATAACTTAATTCAGTCGGGCGCAAAACTATATTATCCCTAAAGTCTATTTTAGGTTGATTTTTTGAATAAATTCTAAGAACTTTGGCTCCTGTCATTAAATTTATTTCTTTTAAATTTTGGTTTTTAAAATTTGGTATTGAACTGCCATATTCTAAATAAGGATTTGCAACATTAAGCTTTTGAATATATTTTTTCTTATCTTCATTAAAATTATTAAATTTACAAGATATTGATATCGCAGATGTCATTGTAAATAATGCTAACATTGAACTTAAAATAAGAATGTTTTTTTTCATTTTTACCACCTTAAAATGCCGTTGCTGTATGAGTGTGAAAACATAAAATAACTATAGTTAATACTAGTCCTAAACTAAATAAAGCAAATGAAGTATATAAAACTTTCGGAGATTGATTTCGAACATGGATAAATTCCGGAACCAATTCAAATATGGACATAAATGTAATAATTCCTCCACCTAATGCCAAAAACGAAGGGATTATTCAAAACACTTTACTATGTTCAATATATTGGCCAAGCAATCCGCCAATTAACATAAATGGGATAATTAATAAAAATGTTAAGAAGTTGTATAGTATGGCTTTTGATTTCTTTTCTCCATATTGAATTTGACGATAATAAACAACTAAAATTTCAAGTAATATATGAATATTAAATGTTATTATTAAAGGAATATTTGCTTTATATGTTCCACCAATTGATGTTAATGTATAAACATTATATCCCAAAAATAGTCCATCAATAATTCTATGACTCATTAGCATTAAAATAGCGCCTCATGCTGCTCTTGGATTGTCGAAATCTTTTATTGAAAATAAGTGGTCCGAGTGATTGTGTTTATCGTGATCTGCATGAACATCTGTTTTTAAATACTTTACAAAAATGAACCTTCCTAAAATAACTAAAATTAATCCCAATAATGCTGATAAACCAATTATTGCAGCTTGGATTCAAGGAATATTAGTTTCAGAATAAGCTCTTAATCCATGTAAATAGCTATTAGATCAATCAAAACTTTCTTTAAAGAAACCTACAGTGCCAATCATTAGAAACATTCCCGTAGCAAATGCATAAATATAAATATTTTTCTTATCAGTAATTTTTAATTTAAATATAGAAATTACGCCCAAAACTAATGAAGGTACGCCCAATAATATTAAAATATAGATTATTAGATTTACAAATGTTGCTAAAGTTATATTTCCGTTAAATGCACTTGTTCCTATAAACATGAAATTCCTTTCTATTTCGTTTATTATACAATAATTTTTAAACAACTATACAATAAAAAATGATAAAATTGTTATATATTATGAAAAAAAAGTTTTATTTTAATTCTATTTTAATTGCTAGCGCACTTAGCTTTATGCCTTTAATAATTTGTAGTTGTCAAACTAAAAAAGATAACTATATTATAGAGGGTAGCAATGCTTATATAGATATTTCAAAAATTTCTAGAGTTTTTTTACATAGGTTAAGCATAGGTCAGATTGCTGCATTACACAACATGTATAAGATTTTTTATTACTATGATAATTCTAATAAAAAGCAATATTATGACTCTGCATATGTAAATGGCCAAAAGCTATATTTAAGAAATGCAAATAAAAATGTTGAATATAAATTAAATTTTCCAATTAAACCTTCTTGGAAACAAGAAATATCAAAATTTGACAATATTAATATTGTAAAAAGCAATAAACCAAGCGATATTAATAATTTTTTAAACGAATATAGTTTTGATGAAGTAGATTCTGCAGGCGATGTTAATGACGAATGATATAGCATATTAGGCGATAAATGAAAAAAAGACTTTAACAGAAATAATGATCCTTATTTTGAAGATATGCAAACTATTATTTTTAGATTAATTCAAGATACAGAATTAAATTATTCAATAATGAAATCTAAATATTTAATAAATTCTAAAAGAGAAAATGTAATTACACAAAATTTATTTAAAAACAAATACATTCAAGCTAGTTCATGATTAAATGATGAGGAACATGAGCAACATCGTCAATGATTCAAAAGATTTTTAGTAATATATTTGAATAAATTTAATGTCAATGTCGGGGATGTAGATATAGATTGGAAAAATGCAAAAATTGTAAGGTCTTTTTCTGGAACCACTGATTATATAAAATTTAAATTCAAAGATATTAAAGATTGAAACGGAAAATCATTATTAAGAAATGATAAAAAAAATATTGAATATTACATAAATGGTTTTAGAACATATGCAACTAATCAAAAATTTGGTGTTGGCAATCAAGGTTTAAAAGAAAAACTACCATTATTCAACGATTACGTGCCAAATCCACTTCTTGAAATTGATGGTATGAAATTTATGAACATTATTGATAATATTAACTATTTTGTTAAAGGCGCAACTGATATTAATTATTGAAATGCAAAAGGATTAATGTATCTTTTTCAAAGCTTCAAAAATGAACCTGGTTTCTTTAAATTAGCAGTTCCTGAATATAAAAAAAATGAAGATAAGGAATATAAAATTATTGATTTTGAATATACTCCATATTTTAAAACAAATCAAATTTTTAAAGCAATAGTAAGGGTATTTAAACTAAATGGTACATATAAAGATTATGTTTTAATATCTTCCAATTTTGATGATCACGGTCACAGATTAAAAGGTTTAATTACTAAAAATGCTTTGCCAAATGAACTTAAAGCTTCTGATATATATTCAATTAGAGCAGATAGTGAGCCAATTAAACAAGGAATCAAATTAGATGATTTCATTGATAAGAATAATAAAAATAGTGTTTTTAGATATATGTTAAATGAATCTGCAAAAAAACTAGAAAATATTTTTGAATATTGAAGCAATAATAATAAGCAAAATTATGAAGTTGCTAATTTATTGCTTGCCAAAGATCCATTCCAATTAAAATTACTAGCTTCTTATTTAAATAACTACTTATTAGCATATGCGCTTGAAAATAAGGCAGGTGAAGTATTTAGTGGAATTAAACGCATCGATCTTAACATAGTTAAATTACCAAATCAACCAATTGGAAGATTATATTTAAAGATGGATTTTGTTTCATATGCCAATAACCAAGATTTCAAATTCAAATCAAAAAATGAAAAAATTGTAAAAAGTGTTTATTTATATTGAAATGGATTTAAAGGATATGACCAACAAAAATATGGAAATGAATATTTTACCATTGATAAAATTACAGACGGAGATAATTAAACATGAAAAAATCAATAAAATTGCTATCCTTGCTTTGTACTTCTACAATAATTTCATTGCCTGCTATTTCTTGCACTATCAAAGTAAATCATGATAACGAAGACAAGGAAAATTTAGATTTTTCAGATGATTTTAGTGGTAAAAAGCAAAAAAATGAATTCAATTTCAAAAATAAGATTTCCCAAAATATTTATGATTTGTATCAAAATAAAATTGCTATTTTATTTAACGATGTAAAAACTAATTACCGGGGATATAAAAAAATATTTGGATCTTTAACAAGAAACTTAAATACTCTAAGGGCTAAGATTAATAGCTTAAGATACGAACAAAGTTTAAAAGAAAACAAAAAAATATTAAATGATTTTTACAATAACTGATTAGAAATAAAATCATCAATGCTAAAAAATAATCCATTTGCATTATATTTATTCAAATATAGTTTAATTTTTTCAGATGTGGATGCTGTATTAGCTGATACAAATCTTGTTTTTGAAACTTCTGAATTTTTAAAATATTTAAAGACAATTGATGACAGACTTGAAGGTAAAGACATTGATCTAGGTCAAACTCAACATGCATTACAAGCGGTTTGAGATTTTATTAAAGCGCATATATTCAATCCAAATAAATTAACCAAGAAAGAAATGCTAGATAAAATTAACATTGAAGATGATAAAAACTCGCATAATCACTCACATGCAATCATAAATTTAACATATGAAATGGGTTTATGGCATGAAATGCTAGCAAAAAATAAACTTAAAAATTACGAAGAATTTCAAAAAGAATATAAAATTGCTAAAAAACACATAATCGATAATATAAACAATATTGATGTAAATAATAATTATTTAGCATTAAAAAAAGTCCTAGATAAAGATTGAAGTTGGGGAAATTCTTACAACTTATTAAATGATTCATTTTTTACTAGAGCAAAAGAAATTTTAAAAATACTAAAAGAAAATTTGATAAGTATTGCTAAGATTGTGGGTTTAACTGATAAAATCAATTTAGACTAAATCTTTAAATCATAATTCCTTTTTAAACTAAAAATAAACGTAACTTGTTATCTAGTTTAGTTATTTTTAATAAAAATAAAAATATTTCTTAGTCTTATATTGCTAAGAAATATTTTTTTAAATTATTAATCTATTTAATTTTTGCTCTTTTGATCGCTTAATGGATCGAAAGCAACGTAAGGATATTTATCTTTTAATGTAGATCATATGTATGAAGTATCAATGTTTTTGTTGGCCTTTTGAATCGCTTCACATTGTTGATATGAATTTATTGCATCATATATTGCTTTATATGTTGTAGGGTTGTTTTTTTCTCATGCAAAATATTCTTTGATAACAACTTTATCAATTTTGTTTTCAGTATAATTTGAAAAAAAGCAATGAACTATTTCATGTAACAATGAATAAAATATATATGCTTCAGAATTAAACATATCAGAAAGGAAAATATACCTTTTTGCTCCCTTTTTTAAAGCAATAGCCCTTATCAAACTATTTTCAATAAAAGGCATCGTTACTAGATTAATTCCTTTGCTTAATAAAACTTTTTTAATTGTTTCCATTTTTTCTTCAAATGAACAATGTGTATTAGACATGATCGTTAAAACTTTATGATAAAGTGAATTAAATGAAGATTTTCTAAAAATTCCCACGTTTTCGTCAATATTTTTATCAATTGAAAGTAATTCGCAAAATCTTATTCAAACTTTTGTATTTGGGTTTGAATATGTTCATTCTTCTGCCGAAACATTATCTTTTAAATATTTATCATAGTTAGCCAAATCAGCAACACCAAAAAATCTTTTTAGCATTATTAATTTAACATGTAATGGACTATTTTCATGAATGCTAATGTTAGCTGCCAATGCAAGCTCTGGATGATTTATTAAAAAATCAATACCATGCTTCTTTAAATCATCTTCAATATTCTTATTTTTCTTTAACGTTTTTAGGTTATTATAAATATGATAAACTTCTGTTAATGTGCCAATTTCTAAGTGAAATGCATATTCTAGCGCTTCAATAATAGTTGCACTAACGTTATTAACTTCATTATTCATAATTGAATTGATATGTTTTATTGATAATCCAAGTCTTATAGATAATTCTTTTTGAGTCATTGAAAACATTTTTAAATAGTATTTTATTTCATCTGTAAAATCATTGCCATGAATTTTAACGTGCTTCATATCACAGATGTGCTCTTCAATCATAAACCCGCCTTAACTTAAACTATTTTTTTATTTTTTATAACTATATTATAATTTTTTTTAGTTAAAAAAGTTTATTTTTGATACAAAAAAATATACAAAAAAGTATTTTTATATTGTTTTTTTGTATATTTTTTTAAAATGATAATTATTGTTAATATATTAAATTTAATATTCCTAATCCACTATATCTTTTGGTGCAACTGCAAAAGGTAGAATATCTTTCATTTTATAATGCTTTTGTTGCTTTAAATCAAAACTGTATAAATATACATCGCCATCTGGTTTTAAAAATTGACTAATAACTTGAGCACAAGCACCGCAAGGATATAGCAATTTAGACAAATTGCTAATTATATGTATTTCTTTAAAATTACCAACTCTAGCGCCATATGCTACAGAACCAAATAATGCACTTCTTTCAGCACATAATCCAGATGGAAATGCAGCGTTTTCAGTATTGACTCCCCAATATTCTTTACCATTTTCTTCAATTGCACAACATGCAACTTGAACATGTGAATAAGGACTAAATGAATATTTTAATTTATTTTTTAATTCTTCTATTGTCATACATATAATTTTACAATATATTTACAATATAAAATTTATATTAAAATAAACATTTTTTAAAAAGTGTTAAGTATTTTTGCTTAACAGTAAAAAATGTGCTAAAATATATACTAACTTTAAAAATAAAATTATTAATTAGAAAGGAAAAACCATGGTTAAATTAAGATTAAAAAGAACTGGTAAAAAATTCTATGCAACATATAAAATTGTTGCCGCAGATGCAAGAAGCCCACGTGATGGTAAATTTATTGAAGAATTAGGCTACTATGATCCTAATTCAAAAAAATTAGTGTTAAATAAGGAATTAATTATTAAACACTTGAACAACGGTGCAAAACCTTCAGACACTGTACGTAATTTGTTAAAGCAAGACAACTTCTATGCAGACTATGTTGCTTCTAAAAAATAATTTTAATAAATTATGAAGATAAACATTCTGACACTATTTCCAGATTATTTAAATGCTTTTTGCAATCAAAGTATGATAGCAAAAGCATTAGAGCTTGGACACATTGAAATAAACATCATTAATTTTAGAGATTATTCATTAGATAAACATCATAAAGTTGATGATAGTCCTTATGGTGGAGGCGCAGGTATGTTGTTGCAAATCGAACCAATAGATTTAGCACTGCAACCTTTAAAAAATAGTTACAAAATTTTACTTTCACCGCAAGGAAAGGTATTTAGTCAAAAAAAAGCACATGAACTTGCAAAATTAAATGAAATTACTTTAATATGTGGGCATTATGAAGGCTTTGATGAAAGAATAACAAAATTAGTAGATGAAGAAATATCAATTGGAGATTTTATCTTAACGGGTGGAGAAATTGCAGCAATGGCAATTTCAGAAGCAGTAGCAAGATTAATACCAGGAGTATTAAGAAAAAATAGCTATGAAAATGAAAGCTTCGAAGGAAATGGATTACTAGATTATCCACAATATACAAGACCTGCCGTATATAAGGGAATGAAAGTACCAAAAATCTTATTAAGTGGAAATCACCTAGAAATAGAAAAATGAAGATTAAAAAAGGCTCGGGAAATAACCAGAAAAAAACGTCCTGAATTATTAAAGAAAGGGAAATAACATGAGAACAAAATTGTTAGAATTAGTAGAAAAAGATCAAATAAGAGAAGACTTGCCACAATTAAGAGAAGGTTATAACGTAAGAGTTCACGTAAGAATTAAAGAAGGCGATAAAGAAAGAATTCAAGTTTTTGAAGGATTAATTATTGCTGAATATGGCACAGGAATCAATAAATCAATTACCGTAAGAAAAGAATCATATGGTGTTGGAGTTGAAAGAATCTTTAAAATTAACTCACCTTTAATTGCTCAAATCGAAGTTATTAGACGTAATAAAGTAAGACGTGCTAAATTGTATTACATGAGAGATTTAAAAGGTAAGAGTGCTAGATTAAAAGAAATTAAAAACTCAAAATAATAAAAAAATAGGGCGTAAGGCCTTATTTTTTTATTATTTAGGGCGATAAATTATATTACTTTAATTAACAAATTATTAACATTTTATTAACAAAAAAATTCAATATAATTATTGGTAAAAAATTTTGCAATTTAATCAAATTAAATACAATTTTTAATATCTTTTTTTTCGACTTAAACTATAATCTTGTTCGAAAGGAAATATTAAAAAATGAAAAAACACACAAAAATTTTATTAGCATTAGGAAGCATTGCAACTTCAGTTGCTGCAATACCATTAGTGGCAGCAGGTTGCGTAAAAACAAAAAAACCAGAAGATAAAAAATCAGGAACAACTCCAGAAGATCCTAAAAAATCAGAAGGTGAAAAGCCAGGAACAACACCTGAAGTTAAACCAGAAGATCCTAAAAAATCAGAAGGTGAAAAACCAGGAACAACACCTGAAGTTAAACCAGAGGATAAAAGAACAGAAGAACAAAAAGAAATAGATAATTGAATAGAAACTATTAAAGACCAATTTGAATTTTCTCCTTACAACAAAGAAGAAGTATTAAAATCATTAAAAGATAAAACGGGAACATTAGTATATAGTTACATATCTCATAGTATAGTTTTAAAAGATAATAATGATGGTAAAAATTGATCTAAAATTGTCTTTTCTCCTAAGGATGAATCTTTATTCTCGCAATATCAACTAGCCAACCCAACAAATCCTACTTATTATAATAAAAAATTCAAGAATAATTCAATAAGTGGTTTCATTGACTATGAATTAAAAGACAATAAGATAACATTAAAATTTAAAGGAGCTAAATTTGTTGCAAGAAAAGATCCTATTATTGGAACTAAAATTGTAAGCAATGAAATAGATCTATCAGAAACACAATTAGATAAAATTATAAAAGATCTTAAATTTGATATCAAAGACAAAAACAAAGAAATTAACAAATTAACTGCCGATGATGTTAAACTAGAAGGCAATCTAAACGGTGCAAATATTACTTATAATTTTGCAGCAAAAAAAGGTGATGAAACAACACTTGTTTTAACTGCTAAAATTTCAAAAAATTCCGTAACTCAAACTTTTGAAAGAGAAATTAAAGGATTTAAAAAACAAGAAACAACACCCGAAACTAAAAAAGAAGACGAAGAATTAGATCCAAAAGTAAAATTGATCAACAAAAAAATTGAACAAAACAAAGACTTATTTACATTCAAGGTTTGAGATCAATCTAAAAAACAAGATGTAATTGAAAAACTAACAAAAAAAGAAGCTAAATTGTTTTATCTAAAAGGAAATCATAATATTATTATTGAATTTAATGGCCAAAAACCAAATGAAACCTTAAGTCAAGTTGTATTTGAAGGAAAATCCGGAGCATTTTGATCATTTGGTCAAGTTCAATTAGTAAATCACCTTCACCACTACAAAGATGAAAAAGTTGAAAAAGATAAATTAGAATTATCTTACTACCTAGAATATGAAGTAAAAGATAACAAAATTACCATAACTTGAGCTGGTGGACAAAAAGATTATGGAATAAACCCTTTTATCTTTGGAACTAAAACTGCTTCACAAACAATTGAATTAAAATAACAAAACATAAAAAAGTGGGCAACATTGCCTACTTTTTTATATGTTTTTTTTAATATGAATTTATTAAATTCTTTTAAGTATGTAAAAGTCTTTTTTACCTTTTTTTAATAGCGCATATTGACCATTAAATTTATTAAATGTAATTGAATAATTTTCATCGTTTATTATATTGTTATCAATTAAAAAAGTATTTTTTGAAATAAATTCTCTTGCTTCTCTATTCGAAGACACAATTTGATTATTTTTTAAAATTTCAATAAATTTTTCGCCTTCTTTAACTTCAATACATGGTAAATAACCTAAAAGCATCGAAATATCTTCTATGTTTAATTCTTCGTTACTTTTGTTGAATAAAATTTCACTTATTCTAATACATTTGTTTGCAATATCAACTCCATGAATGTCTTTAACCAATTCAAAGGCTAATTTTTTTTGGGCAATTCTTTTGGTTTTATTTTCATTGTGTTGAGTCATAATTTTGTTTATTTCTTCAACTTCTAAAAATGTTAATCATTTTAAATATTCTTCAATTTTTACATCACTTTGATTTAATAAGAATTGATATATTGAATATGGGCTGGTCATATTTGGATCAAGTCATAATGAACCTCCGCCAGTACTTTTACCAAATTTTTTGCCATTTTCATCAACTAGTAAATTGAATGTCATGGCAACAGCATCAGCAGATTCTCCATGTAATTTTCTAATAATTTCTAGTCCAGTTGTAATATTTCCTCATTGGTCTGAACCACCCATTTGAATTTTTACATTGTGATTTTCATATAGACATTTAAAATCTCAACCTTGAAGCAAGGTATATGAAAATTCTGTAAATGTTAAACCATTTGCAAGTCTAGTAGTAATAGAGTCTTTTTCAAGCAAATAATTTACATTAATTAATTTACCAATATTTCTTAAAAAATCTACTACTGTTCAATGTTTATAAAAATCTAAATTATCAATTACTTCTAAACCAAATTTTTGTAATTGATTTTTAATTGCATTTTTATTTTGTAGTAATGTTTCTTGATCTAGAAATTTTCTTTCAGAATTTCTAAAACTAGGATCACCAATCATTCCGGTTATTCCACCCAATATTGCAATTGGCTTGTATCCTGCTTTTTTAAAACGTAACAAACTTAATATTTGTAGATAATTTCCAAGGTGTAAACTTTTAGCAGTTGGATCAAAGCCTGTATAAACACCTGTTCCTAGTGATATATTATTAAATTTTTCTTCACTAGATATATTATTTAAAACACCCCTTGATTTCAAATCATCTAATATATTTTTTTGCATATCTATCCTTTTTGTTATAATGTATTAAATTATTTATTAAAAATATAAGTAATATAGTAATTATATATTAATTTTGTTTATTTTATGGCTATGAATCTTAAAATAAACAAATAGAAGGATATTATGAAAATTAAAATTATTATAGACTCATCAGCAGGAATGTCGCAAGAACAAGCAAACAGCTTTGGATGAGATTTAATTCCAATTCAAATTGAAGTAGGAAATGATATATACAAAAGTGGTATAGATTTTGATACAGACAAATTTGAAGAACTTTGAAGAAATGACAAAAAAATAGATGCCAAAACTTTTGCAACTCCCCCAGGAATAGCCATGCAAGTTATAGAAAAATATTTTAATGACTACGATAAAATTTTAGTTTATCCAATTTCAAATGCGCTTTCTTCGCAATGTGCTTCACTAACAGCACAATTTGCTTCAAATCCAAAAGTTCATGTAGTACAATCAAATAAAATTTCTTATCTGATTGTTAGAGACTTATTAATGTTTGAAGAATATATTAAAGAAGGAAAAAGCTTTGATGAAGCAATAGAACACTTTAAAAATAATAATGAAAGATTACTTCTAATTCCAGAGTTTAACGATGCCTTGCTAAAAGGCGGACGTTTATCAAAAGCAGCAGCCGCAATAGCAAAATTATTAAAAATAGTTCCTATTATTAAATTTGATAATGGAATTTTAGAAAAAGATGGTATTGGTAGAATCTTTAAAAAAACTCTAGAAAAAAATTTAAAAGAATTATATGAAAATACAGATAAAGATAATGAAAACAATTATCTATTAGTTGTAAATGCAAATAACGATAAAATAGATGAATTAATTCAGTCATTTAAACAAATAACTAATAATTTTCCAAGGATTTATACAATTCAATTAAGCTGCGACATTGTAGTTCATGCTGGTATTGGCGCAATCTGTTTAACATTTGCCGAAGTTAGTCCTAAAATAAAAGATAAATTCTTTTTATATTTTAAAAAACAATAAATTAAAAATACCAGGTTATTACTTGGTATTTTTAATTAGTTTATTTCTTGCTTTCAATTGGGTAAACTGATGCAAATTTTCTGTTAATTCTGCTTTCGAATTTTACAATACCATCGATTAAAGCAAATAAGGTGTCATCTTTTCCTCTACCAACATTTTGGCCTGGGAAAATTTTTGTTCCTCTTTGTCTATAAATAATGCTTCCTGCTAAAATAAATTGGCCGTCGGTTGCTTTTACGCCTAATCTTCTTCCCGCTGAATCACGGCTATTGGTGGTTGTTCCGCCTGATTTCGTATGTGCCATAACTATTTTTCCTTAATTTCTGTAATTTTAACTCTGGTAAATGGTTGACGATGTCCTAATTTACGTTTATGAGTAGATTTAGCATTATGTCTATAAACAACTATTTTTTTTGCTTTTCCTTGTTTTTCAATGGTTCCTAAAACAGAAGCTTTTGGTAGATAAGGTGAGCCAACTTGTTCATCAATAACTAAAACTTTATCAAATGAAACTTTGTCACCTTCATTGCCATCAATTTTTTCGATGTAAATTACATCACCGCTTTTAACAAGTAATTGTTTTCCGCCGGTTTCAATAATAGCAAACATTATGAAGCCTCCTTATATTTTGTCACATCCTTAAGGTACTATTGATATAGTTTTAAAACCTTTTTAGTATGGTTACTCTATAAGTAACAAATACATTATAGCAAAAAAATATTTTTTTAATCAGTAAAAAGTATTTCAAAAAATTTCTTTTATTTTTCAAGCAATTTACTTACTTTTTTAATTTCAATATCTAGTTTTTCTATTGAAGCAAATATTTCATAAATTGTTGAACTTTTATCCTCTTCGGCGTTTTCTAGTAATAATATAGTAAATGCATCAACTGTAATATTTTTTAAACTTAATAAATTAATTTATTAAAAACCCTATGCTAATTAATAGTAGTATTGTTTGATTAAAAAAGAAAAACAATATATTTTGGCAAATAAAAATATTATTTCAAACCTTTTGGGTGATTTTAATTGCTTTTTTTGAAAGCATAGAAAAATGTGGGGTTAATCAAAATAAAGAAAAAAACAAGATCATAACTGTCTTGCTTTTTTCTATCATTTCTAAGAAACAAAATTAACTAGTTTATTAAAAAATAGTAAATAAAAAACACCCTTATAAATATAGGGTGTTTGTGTCAATAAAATTAAATTCTTATTTAAATTTTAATTATTCAAAATCGTTTTGATTTTCTTCATCATCAAATGTTTCTTCTTCAGCAACAAGTTCTTCGTCGTTTGGAGAATAATCTTCTTCAACTGATTGCTTATCATTGTCATATTCTTGCGCTGTATTAACAACTGAATCAATATCAATAGGTTCAGAAATTTCTGTGCCGTCAATAGGAGTTGTTGTAAAGTAATTAATTGATGGTTTTATATCATATTTTGAATGTTCTTCAAATGGGAATAATGCTGTTCCTGATGGAATCTTTTTACCAAGAATAATATTTTCTTTTAAGCCAGATAAATTGTCAATTCTTGATGAAATAGCAGCGTGAACTAATATCTTGCTTGTTTCTTGATAAGAAGCAGCGGCAAGGAATGAATCAGATAACAATGGAACTTGTTTTGCACCTTTAATAACAACTTTACCATATGCTGGCTTCAAACCTTTTGAAAGTAGAATAGCATTTTCTTCTTGATAGTCAAAAATATCTACAATAGCACCAGCGAAGAATTTAGAATCTCCGGGTTCAGAAATTACTAATTTAGACATCATTTGACGAATTATAATTTCAATATACTTATCGCTAATTGCAATACCTTGAATACGATAAATTCTTTGAATTTCTTTCAATAAATAATTTTGAACAGTAATTACGTCTGTTAAAGCTAATAATTCTTTTAAGATAATTGGTCCTTCAGAAATCTTTTGACCGATTCTTAATTCATCACCTTCTTGAACACGTAATTTCTTGGTTGTGTTAACAAAAATTTTGTGAGAAACTGTTGTGTTTGAAGATCCTTTGCTTTCAATTGTTACTAAATAGTTTGTATTGTCTTTTTCATCAACTTCAATCTTTGTAACAATACCTTTATATGGTGAAATAACAGCAGGACGTCCTCATGGTTGTTCGTGGGCATCAATTAATTCGATCAATCTAGTGAAACCACCAGTAATATCTTCAACCCCGGCAACACCACCAGTGTGGAATGTACGCATAGTTAATTGAGTTCCTGGTTCCCCAATAGATTGAGCTGCAATAATACCTACTGCTTCACCGATATTAACAATTCTATTTGTTGCAAGGTCTTTACCAAAACACATCTTACATACACCGTTACGTGTATTGCAGCCTAAGATTGATCTAATTTCGACTTCTTCAATACCACTTGCTATTATTCGTTGAGCAATTTTTTCAGTAATTAATTCATTTCTAGAACAAATTTCTATACCCGCACTTGTAACAACAGGTTTGTTTGCAAATCTTCCAACAATTCTTTCTTTCAAAGGAACAATAATTTGTTTAGTTTTTGTATCAACTATGTTTCTTGCTTTATAACCATAGTCAGTACCACAGTTTTCTTCTCTAACAACAATATTTTGAGCAACATCAACTAAACGTCTTGTTAAATAACCAGATTTTGCAGTGTTCAAGGCAGTATCGGTTAAACCTTTTCTAGCACCGTGAGTTGATGAATAGAATTCAAATGCAGTAAGTCCTTCAATAAATGAAGACTTAACAGGAACTTCAACTGTTGATCTAACAACACGGTCGTTTTTAGCATCGGCCTTAGTAGTTTTAGTATTGTTAGCCATCAATCCACGCATACCTGCTAATTGAACATAGTTAGAAATGTTACCACGAGCACCTGAATTAATCATGGTAATAACTGGGTTTTGAGGATTTTCTTTAATTATTTTTTGTAGGTCTTCTTGAATTAAATCCTTAACTTCTGCTCATTTTTTAATAGTTAAAGAGTAACGATCATCATCAGTTATTCAACCTTGTTCATAATAGTGTTTTAATTTAGCAATATATTCATCACCTTGAGCAATATATTCTTGCTTTTTATTAGTTTCTAGAATATCACTAAACGAAATAGAAATTCCAGAATATGTTGAATAGTCAAAACCTAATTGTTTAATACTATCCAATATTGAAGCAACAATGTTTGTATATCTAAATCATACAGTGTCTAGAATTTCTGCTTTTTCAGCTGGACTAAGTGGAACTTCTTGTCCATTTGCCCTAGCAGGATTTAACATTTCAATCTTTTGCTTTTCTTGACTTGTAAATTGAGCTAGCAACATTGCATGTTCATAATCAATTTTTCTATTTTCATAATTTTTAACATGCAAGAATTGAATTATTGAATCATTAGCATCACTTGATTCAGTTAAATTATTAATAACTTCTGCAATATCAGACATTGAAATTTGAGCAACATATTTGTCAAAAACATAACGTATTATTTTAGAAATATTCTTTTTGTTAAATGCTTCTTCAATAGGCATATTTTTGATATATTCAGGAATATTAGTACCTGCTTGAACGACATATTTTGCATTGAATGTTTGATTATAATCTTCCAATGAATTTGCCTTAGCAACTTTATTGTCGTAAATAAATGGAAATGTTTTAGGGAATATATTATTAAAAATAAATTTACCTACTGTTGAAATTATATATTGAGGACCATCAAATGGGAAAATAAGTTTTGGAGTTTTAACTTCTGAAGCAGGTAATGCTATTCTAGTGTGCAATGTAACTACTTTATTTTCTAATGCTCTAAGCATGTGTTCATAGTTATCAAAAACACGTCCTTCACCTTTAGCTCCTGGTTCTTCAATAGTTAGATAATAAATACCTAAAACCATATCTTGAGAAGGGTTAATAATAGGTTCACCATCTTTAGGTCCTAGAATGTTTTTATTAGCCAACATTAATTCACGACTTTCAAGCAATGCTTGTTCAGAAACAGGAACGTGAACAGCCATTTGGTCACCATCGAAGTCAGCATTAAATGGAGTACATACTAATGGGTGTAATCTAATAGCCTTGCCTCTTACTAAAACAGGTTCAAAAGCTTGAATAGATAAACGGTGTAATGTAGGTGCACGGTTTAGTAATACTAGTCTGCCCTTAATTGCTTCAGCAACATGAGGTCAAATTACTGGATTTTGGTCTTCAATAATTTTTTTAGCACTTTTAATTGTTGTAGCTACTTCTTTTTCAATTAACCTTTCAATTATTCATGGTTCAAATAATTTAGCAGCCATTTCTCTTGGTATACCACATTGGTGCATTTTTAATGAAGGCCCTACAGCAATAACTGAACGTCCAGAATAGTCAACACGTTTTCCTAGTAAATTTTGTCTAAAACGACCTTTTTTACCAGTTAATGCATCTGAAATTGATTTAAATGGACGATTGTCTTTTGATAAAACAGGGTTAGGTGTTCTTCTTTGGTTATCAATTAAAGCATCTACTGCTTCTTGAATCATTCTTAGTTCGTTTTGAATAACCAATGTAGGAGCATCTTTTTCTTGTCATTGTTGCAATCTATTGTTTCTAATAATTACTCTACGATACAATTCATTAATATCGCTAGTTGAGTGTCTACCACCATCTAATTGAATTAGAGGTCTTAAATCTGCTGGAATAACAGGCAAATTATAAATTAACATGCTAGTTGGATCTTGTTTAGATTCAATAAATGCATTAATTACTTGCAAACGTTTATAAAGTTTTTCTCTTGTTTGTTTTGCAGTTGTGGCAAAACGTTCAGGATTATTAATTTCTTCTGCATTTAAACGATTAATTTCATCAGTTACTTTTTGCTTTTCTTCAGCTAAATTTATGTTTTTTAATAAATATTCAATAGCTAAAGCACCAGTACCTATTTTAGCATCTGAATAATGTTCAATTACTTCGTTTAATTCATAAAAATCAATTCCATATTCTTGACCGATTTTTGATGTTGCTTTTTCAATTAAAGAATCTAGGGTTTCTTTAATATCATCATATTCTTCTGTACCTTCTTGAAATCTTTCAAGCAATTCTTCTAGAGCATTGCGGTAAATGATTGCTGCTTCATTGATTTCAATAATTTGATTTTTAGGTAATGATTTTAAGCCACCATCTTCTAGAACAATATGATTTTTGTAATAAATTAAATTTTCTAGTTCTTCACGTTTAACCACGTCAGAACCATTTGAAGATTTTAAACCAAGCAATTTATAAATAATTGAGTGATCAACTTTAAAGTATCAAAAGTGAACAACTGGACTATTTAGTTTTATATGTCCCATATGGGTTCTACGTGCTATTTTAGGCAATATTTGAACATGTTCTTGTTTACATAATTCAGTTCTTGTACAATAGCTTCCTTCATTAACTTTTTTGTATTTGAAACCACATACAGGGCAACGATAATCAATCATTGGTCCAAAAATTATTTCATCAAATAATCCACCACGTTCTGGTTTATATGATTTATAGTTAATAGTTTCTGGCTTAGTAACTTCACCATGAGACCAAGTTTCAACATCTTGAGGAGTTGCTAATGCTAATGAAATTTTTGAAATTTTTTCTTCATCTAATGATTCGTATTTTGAAATTTTTTTCATATTATTCTAAACTCCCTTCAAATTCTTGGAAATATTTTGTGTCTACTCTTTGATCTTCTTCTGAATCATCTACGACATTATCAGATTTTTGATGTACTTCTAATTTAATTCCTAAACCACGTAATTCATATGCTAAAACATTAAATGATTCAGGTGTTCCTGGTTTAGGCAATCTTGCATCTCTAGCAAGAGAGTTATATAGTTTATTACGTCCAACAATATCATCAGATTTATATGTTAATAATTCTTGTAGAACATTTGAAGCTCCATATGATTCAATAGCCCATGTTTCCATTTCACCAAATCTTTGACCACCATTTTGACTCTTTCCACCAAGTGGTTGTTGGGTAATCAATGAATAAGGTCCTACTGAACGTGCGTGCATTTTGTCATCAACCATGTGATACAACTTCAAATAGTACATAATCCCTACAGAAATTGGGTGATCAAATGGTTCTCCAGTGGCCCCATCGTATACTATTGATTTTCCTGTTACATCAATATTTGCTTCTTCAAGAATATCACGAATTTGGTTGTGTGAAATTCCATCAAATATTGGTGTAACAAATTTTGTATTTAATTTTTTAGCAGCAAGTCCTAAGTGCAATTCTAGAACTTGACCAATGTTCATACGTGAAGGGACACCTTGAGGGTTTAAAACAATATCTAATGGTGTTCCATCTGCTAAATAAGGCATTTCTTCAACAGGCAATACTATTGAAACAACACCTTTATTTCCGTGACGACCAGCCATCTTGTCGCCGACTTGAATTTTACGTTTTTGAGCAATTGATACCTTAACTATTTTTTCAATGCCATCTTCTAATTGGTCGCCAAATTCTCTACTTAGAATATCAACAGCAACAACAGTTCCATTGTGGCCATGTTTTACTTTTAGAGAAGTATCTTTTCTTGAGGCAGTTTTTTGACCAAAAATTGCAGCCATTAATTTTTCTTCAGGAGTTGGGTTTTCTTCACCTTTTGGAGAAGTTCTTCCGACTAAAATATCACCTGCTGTTACTTCTGAACCAACTCTAACAATTCCATTTTCACCTAAGAAACGTTTAGAATAGTTTGAAACGTTAGGAATATCTGCAGTTAAAATATCTTCGCCAGCCTTTGAATGTCTAAATTGAATTGATTGTTCTTCAATGTGAATTGATGTATAAACATCATCTTTAACCAATCTTTCAGATAAAACAACGGCATCTTCATAGTTATAACCATGTCAAGTAGTAAAACCTACTAGAACATTTTTTCCTAATGCTAATTCTCCATCTTTCATTGAAGGACCATCAACTAATAAATCTCCTGCATTAATTTGTTGGCCAACTTTAACCAAAGGTACTTGGTGAATTAATGTACCTTGGTTACTTCTTTCAAATGTTCTTAAATGATAAACATCACTTTTTGAACTATTTTCAGGAGTTACTTTAATAACGTTAGCATCTACATAGTTAACAACCCCTGATCTGGTTGCTCTAATATTGGTTGATGAATATTTAGCAATGTCAGCTTCAACTCCTGTAGCAACTAATGGTGCTTCAGGGAATAACACTGGAACAGCTTGACGTTGCATGTTTGAACCCATCAATGCACGGTTAGCATCATCGTTTTCCAAAAATGGTATAGCCGAAGCAGCTATTGAGGTCATTTGACGGTTTGAGACACCAATAAAATCTACATCTTCAGCATTTACTTCTAAATATTCATTGTCACGTCTTACCATTACTTTTTCATCAACAATTACATTACCTTCAATGTGAACAGTTGATTGTGCAAATGTATATCCTGTTTCTTCAATTGCGGTAATGTAAACTGGTTCTGAAAAATCTACAACTTTTTTATTTACTCTGAAATATGGTGATTGAATAAAACCATATTTATCAATTTTAGCAAATGATGCTAAATTTAAAATTAAACCAATGTTTGGTCCTTCGGGTGTTTCAATAGGACAAATTCTTCCATAATGTGTAGGGTGAACGTCACGAACTTCAAATTGTGCAGTATCACGGTTCAACCCTCTTGGTCCTAGGGAGGTTATTCTTCTCTTGTTTGATATTTCAGCAAGAGGATTACATTGATCCATAAATTGAGAAAGTTTTGATGTGTTAAAGAACGATTTAAATTGATTAAATATTGGTTTATTGTTTGTAACGTTCTTAGCAGTAATCTTATCAATATCTTTTGCCGAGATTCTTTCTCTTGTATTTTTTTCTAATTTTAATAGCCCTATTCTAAATTGATTTTGTAATAATTCGCCTATTGTTACAATTCTTTTATTAACTAATGAGTCAGGATCATCATCAACCCCAACCTTTGAAAGTAAATTAAAATAATACGAAATCGTTGCAATTAAATCAGGAATCAACAAGAAATTTTCTGTTGATGTAGGATCATTACCTAAAACCAAAACTTTTTGGTCATGCAACATATCATTTTCAGTTGGATATATTCAAATGCTTGTTACAAAGATTCTACTTGCAAGTTGTTCATTTCCTTCAACTTCAAGTTGAGATCCATATGCATTTTCATCAATATCTGGCAATTTTCACATAGGAATAATTCCTTGCTTAAATTCATCATGAATCTTTCTTGCAATATTTCAAGTAATGAACATTCCTTTTTCATAAAGAAGTTCACCTTCACTAGAAAAAATATCTTCTGCTAAATAAGAATTGGCAATTCTTTCAATAATGTTTAATTTTCTATTTAAAGTGAAACGTCCTGTTTCAGTTAAACTATATCTTTTTTCATTAAACAAAGTTGTAGGAATTAAATTTCTAGCAGAATCAGCTGTCATACGATCGCCTTTTCTAATGATTCTATAAATTGCTTCTTGTGCTTCCCTTGTAACTTCTTCTGCATTAATTCCAGTGATTTTATCACGTTTAAATGTTTCTTCTAATTCTGGAATGTTTTCGCCAAACATTCTTCTAATACCTGATTCACTAAATCCTAATGCTTTTAAGAAAGTTACAACTAAAAATGATTTGTTTTTATCGATATGTATTTTTACAGTATCTGGATTTTGAGAAGTTACTTTATGATAAATTTCTACTCATGAACCAATTTGAGGAATAATTTCTACTTTATTAAACAAGTCGTTGGCTTGTTTATTTCTAACATTAATTCCAAAATAAGCCCCGGTAGATCTAATTAATTGCGAAACAATAACTTTTTCTGAACCATTGATAATAAATGATCCACCATAAGTCATTATAGGAATTTCAGCAAATACTACTTCTGAATCTTCAACTTCACCTGTTTCGATATTGATTTGTCTTAGGTGTGCTTTTAACTTTCCTTGATATGATGAACCTTTTTGTTTAGCTTCTTTAATGCTTTCATATTCAGTTTTTTTAGGATATTCAAAATGTGCTGAATTATGAATGTATTCAATTCTAACTTTTCCATGTGCTTCAATTGGATAGATATTTCTTAATTCTTCTTCAATTCCAACTGTTAGAAATTTTTCAACTGATTCACGTTGCATTTCAAGAAAGTCAGGAGTTTCGAATTTCTTTTGACTAATTGAATAGTCACGTCTTAGGGTAATAGGTCCAAATTTTCTAATTTCGTATTTATCCTTATTCACCATGCTTTACCTCAATTTTTATTAAATTTTGTTTATATTTTTATTTTTTGTTGTATATACGCACACGTGCATAATATACGACAAAAAACACAAAAAAGGCTTTGTGTATTAAAAGCCTCACTTGTGTTTTTTGTAATTAGTCTAAAGTAACTTCGGCTTTAGCTTCTGTTAATTTAGCTTTTAGTTCTTCAGCTTCTTCTTTTTTAGCATTTTCTTTAATTACAACAGGTGCTGATTCAACAAGTTTTTTAGCATCCATTAATCCTAGACCTAATAAGTCTTTAACAACTTTAATAACTGCTAGCTTGTTTCCACCATCTGATTTTAAAGTAACATTAAATACAGTCTTTTCTTCTACTGCGTCAGCTGCTGCTGCTGGAGCTGCTGCTGCTACAACTGCTGTTGGATCAATTCCAAATTCTTCTTTTAATCCTTGAATTAATTCCATAACTTCTTTAATGTTCATTTCTTTTAATGCTGAAACAAATTCTTCTTTTGTTAATTTTGCCATGATAAATCTCCTTTAAAAATAATTATTCTGAAATTTTTCCTTGTTTTATTAATTCATTTAAACCAATTGATAATTGTTTCAATGGACTCAATAATGAATTTCCAAGAATCATAAGTGATTCTGTAAATGAAGGAAGTTTTGATATTTCGTCTAATGTTTTTTCATCAACTACTTTATTTTCATAAATTCCACTTACAATTCTTAATGCAGGGAATTTCTTCTTTAAGCCTACTAAGGTTTTTAATGCTGCTAATGAATCGCCTTTAACAAAGGCATAAGCATTTTGTAAAACTAAACTATCATTAATATTTGCATATTCTGTATTTTTTAATGCTTGTTTAACTAGTCTATTTTTGTAAACTTTTAGCAATGCATTATTTGGAGCTAATTCTTTTCTAATTTCTTGAAATGAAACAACATCCAAAGTAGCATAATTAATTACATATAATGCTTGTGATTCTTTTAAATTGTTTGAAATTTCTTCAACAACTAAAACCTTGGCATCTTTTAATGCGCTCATTATTATTCCTCCTATAAAGATTATGCAATGTACAAACAAGTATAAACATACTAGGTAACATTATTAAGCACTACGCCGTTACTGTCTTCATATATTGCGTTGAAATTATAACAAATTTTTAAAATTTTTATAATTTTTAAATAAAAAAAATAGCCATTAGGCTATTTGTTGTTCTTTGAAAACTGAATACGACTTAATTTAGATAACTATATAGAATATTACTATTAAACCAATAAATTTATTAGTACTGGTCAGCTGAATGCATTGCTGCACTTACACCTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACACCATCGGTGCGTCCACTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGATCTCTTGGGAAAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCACCACACACTGTCCTCTTCCCGGATAACGGGAACAAGTTAGAAGTTCAATGTAACAAGGGTGGTATTTCAACGGCGACTACTCTTAAACTAGCGTTCAAGCATCAACGTCTCCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAGTCTATTGTCGAGACAGTTAAGAGATAATTACTCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCGGGCTTCACATTAATGCTTCGCTAATGCTAACACCTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAACTTACGGGATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTGTGTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAAATCATCTAATTCGCTACTCAGTTGCCCTTTTGCTATGCGTCGTAACTCCCAGTTAAGTTATGCGGATTTGCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCCTTTCGGCCTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAAGCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAGAAACTTCACAACCTTTCTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTATCACCGATGTACCGACTGCATAGTAATACATGATAGTATTCGGAGTTTGATTATAGTCAGTACGGCTAGGCGCCGCCATTCCATATTCAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCACCCCTACCCACAAGTCATCCGGGCACTTTTTAGCGTACTGCGGTTCGGTCCTCCACTTAGTGTTACCTAAGTTTCAACCTGCTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTCACGTGCCCCGCCCTACTCAGGATACTGCTAGGAGATTTATACATTTCGCTTACGGGAATTTCACCCTCTATGTTTAAGCGTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTCTTTATTTTCTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTCCAGACCCAAGGCATCCACCAAAAACTCTTGCTTGTTTAATAGTTTTGTTAATTGTATGACCTATTGTTTGTCTTATAAAATATAAATATTCTATGGTTGATCTAAACTTTGAAATATTAATTAATATATCAATGTCGATATTCAGTTTTCAAAGAACCGAGAGATAAATCTCTCAAAACTGGATACAAATATTTTTTTCCATAACGTAGGTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTCACCCCAGTCACCAGTCCTACCTTAGGCGGTCGCCTCCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCAGTTACCCAGGCAGTATCTCTAGAGTCCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGACTTGACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCACTCCGATAACCTCCACTATATCTCTATAGCTTTGCGAAGGATGTCAAGAGTGGGTAAGGTTTTCCGTGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGCCCCGGGATTTAACTCCAGACTTAACAAACAGCCTACGAACGCTTTACGCCCAATAATTCCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCATCGCTTTCTGACAAGGTACCGTCAGTCTGCAATCATTTCCTATTGCAAATGTTCTTCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGTCTCTCGACCCGGCTAAACATCATAGTCTTGGTGGGCCATTACCTCACCAACTAACTAATGTTCCGCACCCTCATCTTTTAGTGGCGCCTTACAGCGCCTTTCACAACGGAACCATGCGGTTCCATGCGTATCCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATTAAGCACGTGTTACTCACCCATTCGCCGCTAGGTTATTGCTAACCTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTTATAAAAAAATTTGAATTGGTTGATTATAAATAAAAAAATAAATTGACGTTAATGGTATTCGTATCCAGTTTTCAAAGAACTATCTCGTTGCCATTTTCTAATTGGCACGTTTATAATTATACATATTTTTTTGTTTTTTTATAAAAAAATAAATTTATTTTATTGTTTGTTTTTTTATAAAGTTTTTAGCAATCAAAATTGCTTGATTTATTATATAACTTTAATTAATTTTTTTTGTAAAAAATAGATATTTTTTTATTTACTTATATATTTTTTAAGAAATGCTTCAATAATTACTTATTAGTAATTTAATTATTTATTTTTTTAAATTGAAGAGTTTGATAAATAAAAATTCTCTCGAATAAATCAAGAGAATTTTTTATTATTTAATATTATTTAATTTTATCGTTAATTGCTTCTTTAATAACTTTTCTTCCTCTATAAAAACCGCAGTTTTCACATGCTTGGTGAGGAGTAATTAAATTCTTACATTGTGAACATTCAACTAATGTTGGAGCCACTAGAGCGTGATGTGATCTTCTTAAATGTTTTCTTTGTTTTGAAGTTTTTCTTTTTGGTACTACTGCCATAATTTTCTCCTTTAAATAATCAAATTATACCAATTCGATTATTGCCATTTTAGCATTATCACCAGCACGATTGGCTAATTTTGTAATTCTTGTATATCCACCATTACGTTCAACGTATTTTGGCGCCACAACATCAAATAAGTATTGTAATGAATTTTTATTTTTGTTTTCTGTTGCAATTAATCTTAAATATTTGGCTGCTTGTCTACGCGAAGCTAGGGTATTTTTCTTACCTAGAGTAATCATTTTTTCTGCATTTGAACGAATTCTTTTAGCACGTTCTAATGTGGTTGTTACTTTGCCATTAATCAATAAATCTGTTACAAGTGATCTTTCAACGTGGTCTCATCACTCAGTATTTCTACGAAATAGTTGTTTTGGATTTGCCATTGTTATTCTCCTTCTTCGTTAGCAGCATATTCTTTGTTAGAATCTTCATTATTTAATTGACTATCAAGTCATTCTCTACGACGGTCAATAATATCTTGTACTGATTTTTTACCCAAATTTTTAATATTTGAAAGTTCTTCATCGCTTAATTTCATTATTTCATCAACATTATTAAATCCAGCACGTCTTAAAGCATTCAATGAACGAATTGTCAAATTCAATTTATCAATAGAAACTGAAACTTTTGGAGTTTTTTCATGTTTTTCTTTTTGATCATCGAATAAATCAATTGTTCCTAATGCGTCAATGTTTCCAATGATTTGGAAATGAGCAACTATAATCTTTGCTGCTTCTTCCATAACGTTTTTAGCTTCAACAGTTCCATCGGTTTCAACTTTAATTTTTAATCTTTCTTCAATTATGTTAGAAGTAGAATTTAATTCTTCAAATAAAATCGCAACTTTTTTAATAGGACTGAAGTCACTATCCATTGCTAAAAATTGACCATTTTTGATTTTTGATTCTAATTTTGGGCCATATTCTAGAATGGTCTTTTTATTGTCTTCAAATGAAACATAGCCTCTACCGGTTCTTAAATATAATTCAAATTCCAATGAACCATTTTTTGATATTGTTGCTATGTATTGATCTTTATTAACAATGTCAAGTCCAGGGATAGTTTCAATATCTGAAGCATGAACTTCCCCTTCTTTTGTTGATTTAAATGAAATTTTAGCTAGATTATCTTTTTCAAATAATTCTGGGTTATATGCAAAGCGAATTTTTCTTATGTTTGCAACTAATGTAATAGCATCTTCTCTTAATCCTTCAATTGCAGTAAATTCATGTTCAACATGACTAATTTTTATTGCAAATGGAGCAACAGAAGTAATTGAAGAAAGTAATGTTCTTCTTATTACAGTTCCTATTGTATTACCATAACCACGCATTAGTGGTTCAATAACAAATGTTGTATTAAAATCTGATACTTTTTCTGCTACTAATTCTTTATATGTAATTTTTTGTATTTTCTTCATATTTATTCCTTAAAAAACAAAATAACAAATTATCCACGTTTAAGAATCTTTTTAGGAGGACGAGTTCCATTGTGAGGAGTAGGTGTTACGTCCTTCACTTCTTTAACACTTAAACCACTTGTTTCTATTGATTTTCTAGCAGTTGATTTTCCAGGACCAACACCTTTAACTAAAATTGTAACTTCCTTTAAACCAAATTCTTTTGCTTTTTCAACTGCAGCAGCAGCAGCAAGTCCTGCTGCATATGGGGTTTTCTTCTTTGTGCCTTTATATCCTATTGCACCAGAAGAACTTCAAGCAAAAACATTTCCTTTTAGATCTGAGAAAGAAACAATTGTGTTTTGGTAAGTTGAGTGAATATGTGCAATACCTTGAGTAATAACTTTTTTATTTTTCTTTGCCATTATTATTTACCTTTCTTTCCTGCTATTGTTTTTCTTGGACCTTTTCTTGTTCTTGCATTTTTCTTTGTAGATTGACCACGTACAGGTAATCCCTTACGGTGTCTAATACCACGATATGATTTAATTTCCATTAATCTTTTAATGTTTAAATTTATTTCTCTTCTTAAATCACCTTCAGTGGTGTATTTTTTAGCTTCATCTCTGATTCTAGTTAATTCTTCTTCGCTAAGATCTTTAACTCTTTTTTCGCCGTCAACATTAGCATCTTTTAAAATTTCTTTTGCTAGTGAAACGCCAATACCAAAGATGTAAGTAAGTGAAATACGAACTTTTTTATTATTAGGAATTTCAATGTTTAAAACTCTGGCCATGTTTTATCCTTGTCTTTGTTTATGTTTTGGGTTAATGCAAATTACGCGATTAACACCATGTCTTTTAATTATCTTGCAATTATTGCAAATACGCTTAATAGAAGCTCTGACTTTCATATTTCTCCTTATTATTTGTGTCTAAAAACAATCCTACCTCTGCTCATATCATATGGACTAAGTTCAACGTCAACTAAATCTCCAGGGATCATTTTGATATTGTGAAGTGACATTTTACCTGAAATAGAAGCTTTAATAGTAAGTCCATTTTCTAATAAAACATCATAGTCTCTTGATGTGTGCATTTTAGTAATTTTGCCTGACATTTTGATTGCGTCTTTTGCCATTAAATATTGTCTCCTGAGAGTATATATGCTTGGTCATTGTCAATTAAAACTGTTTGTTCATAATGAGCAGTGTTATAACCAAGAGGATCATAAACTGTTCATCCATTTCCCTTGACCAATACATTTTTAGATTTTTGTAAAATCATTGGTTCAATGCAAATTACCATACCATTTCTAATTAGCTGTCCAGTACCTGGATAGCCATCGTTATAAACATATGGATCTTCATGCAACTTTCTGCCTATTCCGTGACCTGTATATTCATCTGGAGTAAAATATCCTCTATCACGAATTAAATTTCCTATGGCTGCAGAAATATCACCAATGTGTTTGCCAACTTTAATTGCATTAAAGCCAGCATAAAAAGCGTCTTTTGCGCAGGCAATTAATTTTTTGTCTGCTTCAGTTATGTTGCCAACTCCTTTTGTAAATGCTGAATCAGAATAATAGCCGTCATAAATTGCTCCAGTGTCAATTTTGACTATATCTCCATCCTTTACAACATAATCACTAGGAATTCCATGAATTAATTCATTATTAACAGAAATGCAACATGTATTAGGGAAACCATATTGTCCTAAGAATGCTGGTTTTCCACCCCTTTTTCTTATTTCTTTAAAAGCTACGCTATCAATTTCTTTTAAAGAAACGCCTGGTCTTATAAAGTCGTATAAAACAGTTTTCACTTCTGCCAGGATTTTACAAGCTTTTTTGATCTTTTCAATTTCAAATTGATTTTTTATTATGACCATTTTAAAGCCTCTTTTACATCATTATATACTTTATCAATCGTTTGATAACTATTTATCTTTAAAAGTATACCAAGTTCTTGATAAAAATTGATCAAACATTTAGTTTGTTCATTATATATTGATAAACGTTTCTTTATTACTTCTGGTTCGTCATCTGGTCTTTTTGTTACTTTTTCGTGGCATTTAATACACAAATCACCAGGTAATGGTGGAAATGATTGCAAATGATAAATGGTTTTACAATTTAAACAAATTCTACGTTTTGAAAGACGGTCAATAATTTGTTCATCAGTAATTTCTAGTAAAATTACTTTATCAATTTTAATTCCAACATTTGCTAATGAAAGTAAAAACTTTGCTTGAGCTAATGTGCGGGGATATCCGTCTAATATGAAGTGTTTCTTTTCTCTTATTAATTCAGTTAATTTTTTTTCAACTAGTTGATTTGTTATAGAATCATCAACATATGCTCCCGAATCTAAAATAGATTTTATTTTTAAGCCAAGGGGAGTTTGATTTTTAATTTCTTGTCTAAACATGTCCCCAGTTGAAATTTGAACATAATCATTATTTTGCACCATTTTTGTTGCAATAGAGCCTTTACCAGCTCCCGGTGCGCCAAGCATAATTATATTAGGAAATTTTTTTGCGTGTTCGTTGTACACTTCTTGAGATTTTTCTATCATATTAAATCTTCTTCTTCCTCTCCATACATTTCTTTTAATTCTCTTATCATTTTTCTCTTTCTTGATAATTCTTGTGACTTGTAGCGGGCTTTAATTTGTTGAACAGTTTCATAAGCCGTTGAAACTAGTATCATTATGCTTGTACCGCCAAATGCTATTGAGGCTGGCATGCCTAGCATTTGTTGAATAAATTGCAAAGCGCCTAGCAGCAATAAATATCCTGAAGAAAAGAATGATAAACGTAAAATTACATTCAATAAATAATCTTCTGTTTGTTCTCCGGGTCTAACGCCAGGAATAAAAGTACTATTTTTAGCAAAATCTTCACTAATTTTATCAATTCTCGATTGTTGGATTCCCATTAATATTGTTAATCCAAATGTAATTAAAATAAATAGAAAGAATCCAAGTGGTTGAGTAAATTGCAAATTGTTGTAAACTCATTGATAATACTTGCTTGTATTTGGATCAAATAAGTTTGCAATCATTGTTGGCACACTCAATATCATCATTGAGAAGATAACTGACATGATACCAGCAGGATTTGCTTTAATAGGTAAATAACTCAATTCTTTTTCGTTTTTAGAAAGACCCGCACCAACTTGTTGAATTGGAATATGTCTTTCTGCAATTGTAAATATTACAACAATAAATATTACAAGTAAATAACAAAAGATATATACTGCGTAATTTATTGCTTCTTGTAGAACTAAATTATTTTGTTGAGCTGATGGAACATAAAATTTAAATGCACTCTTGAATGAAGGAATTAAAGCACTTGCTATACCAACAAAGATTATTAAACTCGTTCCATTGCCAACGCCTCTATTAGTTATTTGTTCTGATAAAAACAATGAAAAGAATGAGCCAGCTACTAATATTGCAGGCAAAATGAAATATTGATATGCCACCATTCCACTTTGTCCAAATAATTTTTCTAATTTTGGTGAAAAAGTAATTCCAAATCCTTGACGAGGATTAACTAATGCTTTTGTTATCAAATATGCTTGAACTATTGCAAAAACAAAAGTAACTCCGTAAGTAATGAAATTTATTTTAATCCGGCCTTGAGGACCAGATTGACTTAAACGGTGAATGGCTGGGAATGCTTTAGTTTGCAATATCATCATAACTAAACTAGATGATATAAACGGTCCTATTCCTAGCGCGACAACAGAGAATTGTCTTAATCCGCCGCCACCTACTAAATTCAAAATCCCAAAGAAATCTCCTTGGGTTAATGAATCAGGGTTTGCTAAATTGACACCTGGAATTGTAATTGTTCCAGTGGCTAGAAAAAGAGTAATAATGAATAAGGTGAAAAGAATCTTTTTAAAAAGATTATGATTTTTTCATCATTCATTTCAAGTGTTTTTTTTATCAACTAAAAACTTATCTAGTGCTAATTTGCGCTCGATCTTTTCTTGACTAATCTCTTTTTCCAACTTAGTTTTTCTAGCCATTATTTAACCTCTATTTTTCCTCCGACTTTTTCAATAGCTTCAATAGCGGATTGTGAAAGTGTTGGAATTTGTACATTAAGTTTTTTAGTTAAAGAACCCTTAGCTAAAATTTTAACTGGTAATGTTCCTTTAATCAAGTTTCTTGCAGCAAGTGTTTCGTAAGAAACTACTTCACCATCTTGATATTTTGATTGTAAATCTGCTAGATTTACTACTTGGAATTCAACGTGATTTACATTGCTAAATCCACGTTTTGGAATTCTTCTAAATAGTGGCAATTGGCCACCTTCAAATCCTAGTCTTACTTTGCTTCTTTTTGTTTGACCACTTTGTCCTCTACCGGCTTGTTTTCCTTTTCCAGCAGCATGACCACGACCAACACGGTGTTTGTCTTTTCTTGCCCCTGGAGTTGGTTTTAAATTATGTAATTGCATAATCACTCCTTATTTTGATAATAGATGATTTACATCAATATCGCGTAATTTTGCGATTTCTTCAACAGTTCTAACTTTTTTAAGTGCCTTAATAGTAGCTTGGACAATGTTTTGTTTTGTTCTTGAACCATAAGTTTTTGTTGAAATGTCGGTGTAACCTGCTAATTCAACAACAGCACGAACGGTGTTTGAAGCAATGATTCCGGCACCTTTTGGAGCTGGACGTAATTGGATTCTTGATGCTAAGAATTTATCTTGGATTTCATGAGGGATTGTTCCATTTGAAATTGGAACTGAGAAAACATTTTTTTGTGCATCTTTAACTGCTTTTTTAATTGCATCTTGGACTTCATTAGCTTTTCCGTGTCCAAATCCAACTTTTCCTTTTTTGTCACCAACAACAACATAAGCTGAGAATGAAAATCTACGTCCACCCTTAACAACAGTTGTAACTCTTGCAATGTCAATAACTTTTTCTTCAAATTGTTTTGCTTGAGCTTGATTTGCTGAATGATCTTGATTACGATTTTGGAATTGTTTTGGTTTATTTTCGCTTGATTTTAGATCTTTTGAATTTGATCTTTCTCTTTTAAAAGAAACAACCTTTGTGCCTTCTGTTTTATTTTCTTTTAATTCAGGTTTTTCTGAATTTTTAATATTTTTTTCTACTTCAGCCATTAGAATTTAATCCCTTCTTCTCTTAGAGTATCAGCAAAAGCTGCTAATTTTCCGTGGTATATGTATCCTGAACGATCAAACACTATTGATTTTACATTGATTGCCAATAATTTTTTAGCAAAATCTTTTGCAACTAATTTAACTGCATCAATATTGTTTGCTTTATTTTCTAGTATTTGTGTTGATGATGAAGCAATTGTAGTGTGCAATTCATCGTTTACTGCTTGTGCATAAAATGCATGTAAAGATTTAAAAACAACAACTCTAGGAACTAATGCAGTTCCTTTTGACAATTTGTTAGTAATCTTTAAGTGTTTTGCTTTACGTTTTAAATTTCTTGATAACATAATTTATAACTCCTTAGGCTACTATTTAGCAGCTGTCTTTCCTTCCTTACGTCTAATAACTTCATCTTTATACATAATACCTTTTCCAGAATATGGACTTGGACGTCTTACATCACGAATATTTGCAGCAAATTCACCAACAGCTTGTTTGTCAATTCCTGAAATAATAATATTGGTTGGTTTTGGTAATTCAATTTTTAGATTTGATGGAACTTCTAAATTTACAGCATGTGAATAACCAGCAATAATTTCAATTGTATTTCCCTTTAAGGTAGCTTTGTAACCTACACCTTTAATTTCAATTTCTTTTTTGTAACCACTTGAAACACCTATTAACATATTTTTAATGTTTGCATTTGTTGTTCCGTGTAATTGTTTTGTAGATTTTTCTTCGTTAGATCTCAATGTCTTAATTTCGTTGTTTTCAACAACTACTGAAATTAATGGTGAGAATGTATAACTCAATTCTCCTAATTTGCCTTTTACTTCGATATGGTTTTTATCTAATTTAACTTCAACATCTTTTGGTATTTCTAAAACTCTTTTTCCAATTCTAGACATAATTACTTCTTTTTAAGTTTATTATCAAATGAAGGCGATAACTTCGCCACCAACATTAGCCTTACGAGCTTCTTTATCTGTTAATAAACCTTTTGATGTTGAAATAATAGCTGTTCCATATCCACTTAAAACTCTAGGCAATTTTTCAGCAGATGAATATACTTTTAAGCCAGGTTTTGAAACTTTTTTAAGACCAGAAATTGCTGATTGATTTTGATTCATACCTTTGTATTTTAAAGTAACTTTTATTTCTTTAAAATTGGTTTTTTCGTTTTTTACAACTTCAAAATCAACAATATATCCTTCTCTTTTAATAATTTCTAAAATTTTTGCTTTCTTGTTTGAATGTGGCAATACAACTTCATGATATTTACGAGAAATTGCATTTTTAATACGTACAAACATATCTGCAACTGGATCTATAATAATAGCCATAATTATCAGCTCGCTTTCTTAACACCAGGAAGTTTTCCTTCATGTGCTAATTTTCTAAAACAAATTCTACAAATTTTATATTTACGCAATACAGCGTGAACACGGCCACAAAGTTGGCAACGAGTATATTTTCTAACCTTAAATTTAGGTTGGCGTTCGGCTTTAACCTTTAATGATGTTCTTGCCATAATATCTCCTAATTATTGCCCTTTGTAAAAGGGATTCCTAAGTGTTTTAATAATGCAAATGCTTCTTTATTGCTTTTTGCAGTTGTTACAAGAATAACATCCATACCTTTTAATTTACGGATTTTATCAAATGAAATTTCAGGGAAAATAATTTCTTCTTTAATGCCGATAGCATAATTTCCACGGCCGTCAAAGCTTTTTAGTGATGTTCCATTGAAGTCACGTACACGAGGTAAAGCAACATTAATTAATTTTGTTAAAAATGATCACATCTTTTCTCTTCTTAGTGTAACTTTCCCTCCCATTGGCATTCCTTCACGTAATTTTCATGAAGCCAATGATTTCTTAGCAGTTGTTTGATATGGTTTTTGACCTGTAATTTGAGCAAGTTCAACCATAACTTCTTCGATTGCCTTTGAGTTTGAAACTTCGTTACCGGCAGTCATGTTAATAACGATTTTTTCTAGTTTAGGAGCTTGCATTACACTGGTGTAACCAAATTCTTTAACTAGCGCGAAACGAATTTCGTCTAAATATTTTTGTTCTAATGCTAATTTAATTTGTTTTGCCATATATTTGCTCCTTTCTAGATAGTCTTGTTAATTTTCTTCATCACTCTTTGTTTTTTGTTGGTTTTAGGATCAACTTTTCAACCAATTTTTGAACCGATTGATTTTTGACCTTGAGCACCTTTTTTAACCAAATAAGCAACGTTTGAAATATGAATTGGGTATTCTTTCTTTTCAATTTTTCCATCATTATTTTCTTGTGATGGTCTGTGGTGTTTAGTTTTAATGTTTACGCCTTTTAAAACAACAGTTTGTTCTTTTTGATTTGTTGATAAAACAGCAGCAAATTTTCCTTTGTCTTTACCAGCAATAATTAATACCATGTCATTTTTTCTAATTTTAGCTTGAGCCATATTATAAAACCTCCGGAGCTAATGAAACAATCTTTAAAAAACCTTTTTCACGTAATTCTCTTGCTACTGGACCAAATACCCGAGTTCCTCTTAATGAGTTATCTTCCTTAATTAATACAACAGCATTATCATCAAATCTTATATGAGAACCATTTTCACGCATTAAACCACGTTTTGTTCTAACTATAACTGCTTTAACAACTTGGCCTTCTTTTACAATTCCATTTGGAATAGCTTTTTTAATAGTTACAACAACAATATCACCAATATTGGTTGATCTAACAATTGATCCACCTAGGTTTTTAATAATCATAACTTCTTTTGCGCCTGAATTGTCAGCAACGTTACATCTTGAAAATTCACTAAGCATTTTCTGCTCCTTCAATTGCGTGTGATTTGATTTCTACTAGTCTAAAGTGTTTGGTTTTTGATAAAGGTCTTGTTTCTTGAATTTTAACAATGTCACCAACTTTAGCTAAATTTTGTTCATCATGAACTGCAAATTTCTTTGATTTTTTAAAACGTTTTGAATAAAGAGGGTGTTTTTCGTAAATTTCTACAACAACAGTAATTGTTTTTTGGTTTTTAGTTGATACAACTGTTCCAACTAAAGTTTTACGACGATTTTCTCTAACTGTAGTTTCCATTATTTATTCCCTTCTAAAGTTTTTGCTTTAATTGCTGTTAAAGTTCTTGCGATATCTTTTTTAACTAATTGGATTTTGTGGGTTTCGTCTAATTGTCTTGTAGCATTTTTGAAACGTAATGAGAATAGTTCTGCTCTTAAATCAGCAACCAACTTATTTAGTTCGTCAAGTGATTTTTCTTTTAATTCTGCAAATGTCATAGTAATTATTCTCCTTCAACTTCATTAGTTTTAGTTTCTAAAGATTGTTCTTTTGAAATAACTTTTCAAGTTACAGGTAGTTTGTGACCACCTAATCTCAATGCTTCCTTCATGGTATCAACTGTATTGCCTAAAACTTCAAACATTACTGTTCCTTCTTTTACGACTGCAAATCATTGATCTGGGCTACCCTTACCAGATCCCATACGAACACCAATAGGTTTACTAGTTTTTGACATGTGAGGGAATATTTTAATAATAACTTTTCCTTCACGTCCCATATGTCTAGTAATAGCAATACGGGCTGCTTCGATTTGTCTTGCACTAATTCATGCTGATGAAGTTGATTTTAAACCAAATTCGCCAAATGCAACTGTATTATTACGGTGTGCAACAACTTTGTCGTGGCGGATACGGAACATTTTTCTATGTTTAGTTCTTTTTGGTTGAAGCATGTCTTTCACCGCCTTTTTTAAATTCTCTTTTACGTGGAGCTTCTTTTACTTCTGCTTCACTTGTTTTAGGTGATAAAATTTCGCCTAATGAAACTCATACCTTAACTCCTAAGATACCATATGTTGTTAAGGCTGTTGTTACGGCATAATCAACATCTTGTCTTAAAGTATGTAATTTCATTTCACCTTCGGTATATCCTTCTGTTCTAGCCATATCAACGCCATTTAGACGTCCAGCTACTGAAGTTTTAATACCTTTTGCGCCAGCTTTTAATGCTGCTTTAATTGCAAATTTTTGTGCTAGTCTAAAACTACCACGATTTTCTAATTTAATTGCAATTCCTTCTGCTAGTAATCTAGCATTTAAATCAGGATTTTTTAATTCAATTACTTCAATAGTAATGTCAGCTTGTTTTTTAGCAATAACCTTTTTTAATTGTGCTGTTAAGGCTTTGACATTTTCGCCATTTGTTCCAAGGAATGCTGCAGGTTTTGCAGTGTGAATATAAACAATAGTATGTCCTGCTTGATCACGTTTAATTTGTACTTTACCAATTAAATATTCACGAACTTTTTTGTCAAAGAAATCATAAATTTTTTGATCTTCTAATAAATAAGTTGCAAATTTATCTTTATCTGCATATCAAGTTGTGTTATGAGATTTTGTAATTCCATAACGAAATCCATTTGGATTAACTTTTTGACCCATTATTTATCTCCTTTTTTGTTGATTTCTTCAACATTATTGATTTCTGTTAATTCAATAAAGAAATGACTTGTTCTTTTTAAAATTGCATAAGCACGTCCACGTGAGTGAGGTTGGAATCTTTTTAGTGTAGGTCCATCATTTACTAAAATGGTAGTTACATAAAGTTTAGTTGCATCAAGTCCAGCATTATTTGTTGCATTAGCAATTGCTGAATTTAGAAGTTTTAGAATAATTTCTGACGCTTTTTTGTGTGTATGTTTTAGAATAACTATTGCTTGAGTTGCTGATTTATATCTAATTAAATCTGCTACTAATCTTGCTTTACGTGGTGAAATTCTTTGCATGCGAACTGATGCATGTGCTGAATTTTGTAAAATTTCTTTAGCCATATTCTATTTCTTTCCTTTATCTGCACCATGACCATTGAAGGTTCTTGTTGGAGCAAATTCACCTAGTTTGTGTCCTACCATATCATTTGTAACAAATACATCAATAAATGCATGTCCATTGTGAACTTGGAAGGTTAAACCGATAAATTCTGGATAAATTGTTGAGCGTCTTGATCATGTTTTAATTGGACGTTTTGGTGATTTATTTTCAATAATAGCCAAAACTTTTTTCATTAAATGATCATCAACAAAGGGAGCTTTCTTTAATGAACGAGCCATTATTTAGTTTCCTTTCTTCTTCTAATAATAAATTGATTTGATGCTTTTTTAGTTGCACGAGTTTTAACACCAAGTGCTCTTTTTCCTCATGGAGTCATAGGACTCTTTCTACCAATAGGTTGTCTACCTTCACCACCACCATGTGGGTGATCATTAGGGTTCATAGCTGAACCACGAACTGTTGGTCTAATTCCTCTTAAGCGGTTACGTCCTGCCTTACCAATATTAACAAGTGAGTGTTCTTCGTTTCCTACTTCACCAACTGTTGCACGATTAATTGCTAAAACTTTTCTAACTTCGCCTGATTTCAATCTTAGAATTACATAACGACCTGATTCATCTTTACCTAAGATTTGTGCTGATGATCCAGCTGATCTAGCAATGATTCCACCTTGTCTTGGTTGTAATTCAATATTGTGAACAAATGTACCTTCAGGAATGTTGGCTAATGGTAAATGGTTACCAACTTGAATGTCAACATTATCACCTGACAAAATAACTTGTCCAATTTTGATACCTTTTGGGCTTAAAATATATCTTTTTTCACCATCTCTATAAACAACTAATGAAATATTAGCTGAACGATTAGGGTCATATTCAATAGTTTTTACAGTAGCTGGGATTCCATCTTTTTGACGTTTAAAATCAATGATACGATAGAATCTTTTCAATCTACCACCTTGATGACGGGTAGTGATTTTACCTTGATTGTTTCTTCCTGCTTTTGTTGGAAGAGCAACAAGCAATGATTTTTCAGGAGCATGTCCTGTTAAATTTGCTTGATAATCAAGAGAAGACATATTACGACGGCCATTGGTATATGCTTTAAATTTTTTAATAGCCATGTTGTTTTCTCCGTTCTTAAAAATATTGTTATCTTTTAAGAAAATATTAATTATTTTTTAATTTAGTTTCATCTTATATTTATGCAAGATTAACTATTTGTTTTCAGCAGCTTTTTTAGTTTCAGCTGCTTTAATTTTTTCTGCTGCTTTTAATTCTGCGGCAGTAGGTTCAGCAGGTTTTTCTTCTTTAACTTCTTTTTCTGCTGTTGCTTTTTCAGCTTCTTTGTTAGCTTTCTTTAATTCTTCAGCAAATAGAATAATTTTTGAATCTTTTGCTAAATATACAGTTGCTTTTTTAACTGAATTTTTAAAACCTGAGAAACGTCCTAATTTAGCAGGTTTTTTATCGTAATTAGTAATGTTAACTTTTAATACTTTTACATCAAAAATAAATTCAACTGCTTTTTTAACTTCGATTTTGTTTGTTCTTTTATCAACTACAAATGTATAAACGTTTTTATTTTCACGTGATAATTCACTTTTTTCTGTTAAAAGTGGGTATTTAATAACTTCATTTATATTCATTATTTAACCAATCCTTCTAAGTATTTAACATCATCTGAACTAATAACTAAAACATCTGCTGCCAATAAAGATTCAACTGATAAACTAGTTACTTTTGTTACAGTTACATTTGGTAAGTTTCTTGCTGATAAGAACACTGTTTCATTGTTTGAAACTAATAAAACCTTGTTTAGACTAGCAATTTTATCTTCGTTTAATTTAATTAATAATTCACGAGTTGAAATTTTTGATAAAGAATATTCTTTTACTAAAACAGCATGGTCTTTTGCTAATAAAGTTAATGCTGATAATAAAGCGTTTCTTCTTGTTTTTTTGTTGATTTTTAGATTGTAGTTTCTTTCAGTTGTTGGGCCGAATACCTTACCACCACCAACAAATATAGGTGATCTTAGTGATCCAGCACGAGCATTACCAGTATGTTTTTGATCTCATGGTTTCTTACCACTTCCACTAACTTCTCCACGAGTTTTAGTTTTGTGAGTAGCAAGTCTTCTTGAGCCACGTTCTGATAAAATTGCATCAAAAATTGCTTGGTTATAAATTTTTTCTAAACCAAATACTGATTTAGGAAGATTTTCACTTGAAAATTCAACGTGTTCTTCATTATCTTTTTTAACTGATATTTTTGATGGTTTTTTAGGAAGAGTTTTTTCTACTAGATTTTCTTTTTCTAAGTAGTCGATTGCTTCTTCATTTTTAACTTTTTCGCCAGTTACTTTTTCTAAAATTAATTTAGCTTTTTCTAATCCAACTGATCCGCGGTAAGCGCCATCACGGTGGAATCATACTCTAGTATTGTTTTTTGATTTTTCAGAAAGTTCAATAAATTCTGTTAATGCTTCTGAAAAATTTTCAAAATTCTTGAATGATTTTTGGTTAGCCTTTTTTAAATTAAATGAAATATTACGATTTTCATCAAATTTTTCAGAAATGTAGAATTTTTCTAATGTTACATTTTTTGTTGCCATTATTTATTTTCTCCTTCTTCTCCGGCCTTAGCAATTGCTTCATCCAAAGCAGCTTTCATTTCTTTTAATGACATATCCATGTTTAATTCAAGATTGTATTTTTTAGCTTGAGTAAAGATTTCATTTTTAGCCAAAGCTTCTTTTAGATTCAATAATTTAATAACTGGTTTATTCTTAATTGATTTCTTAGCAGTTTTAATGATTAAGAATGCTCCCTTAGCACCAGGAACTGATCCCTTGATCAATAAAATATTGTTTTCTGCATCTTCTTTAACAACTTCTAGGTTTTGAACAGTTACTTGTTCAGCTCCTAAATGTCCTGGCATGGTCATACCTTTTCAAACCCTATTTCCAGAAATATCTCCTAGAGAACCGGTTTGTCTTACAGGTTGTGAACCACCACCACCACCGTGTGATTTAGGGCCAATATGTTGATTTCATCTCTTGATGGTACCCGCAAATCCTTTACCTTTAGAAATAGCTGTTACGTCAACAACTTCTCCAGCGGTAAATAATGAAGCATCTACAGTATCACCTAATGAAAATCCTGACATATCACGGATTTCTTTTACGAAGCGCTTAGGTGTTGTATTTGCTTGTTTAAAGTAATTAACTTCAGGTTTAATGATTCTTGAAGATTTTTTATCTTCCGTTGCTAATTGAAGAGCAACATAACCATTTTTTTCTGATGTTAAAACCTTAGTAACAACATTTGGTTTAACTTCTACTACTGTCACAGGTATTAATTTACCTTCTGAAGTAAATAGTTGAGTCATCCCAACTTTTCTTCCTAAGATTCCTTTCATGATTATCCTTTCAAATTTATTAATTTATTAAATATATATACTATATTCATAAATAAAAAATAATTACTGTTATTGATTAACAGTTTTTAAACTTATTTGTACACCAGCAGATAAATCTAATCTCTTAGCTTTATCAACTAATGATTCAGAAACTTTATCTAAAACGATTAAACGTTTATGAGTTCTGCTTTCAAATTGTTCACGAGATTTCTTGTTAACGTGAACAGATCTTAAGATGGTAATAATTTCTTTATGTGTTGGTAAAGGAATTGGACCACTAATAGTTGCTTTTTCAGCTTGAGCTAATAAAACTATTTTTTTAGCAGCTTCATCTACTAAGTGGGCATCAAAAGATTTAATTTTGACTTCTAGTTTCATATTTGTTCTCCTTCTTCGTAATTCGAAGTAACTCCACACAAGTTCTCAATATGTGAGATGAACAACTAAGCTCGGCATATCGTAACCTCGTGTTTCTTCGTTGTAGTTGCAAAAGAATTATAACAAAATTTTTATTTTTTAAAAAAATAATTTTTTTTGATGAAAAAAAACATGCTTTTTACTTTTGATTTCATTAAAAAATTGGTAATAAATAATGATTATCTTATACTAAAAAATATAGATATTAAAAAGCAATATAAATAATTAATAAAGATAAATACAAAGACATTAATGAAATCAAAAAACCAAATGATTTTTTAGATTTAAATAATAAAGAACATTGACAAAAATTGTTTTTAGACAATGTTTTAAAAAAATGAAATTCAGCGTTTAATGTAAATGTTGATTTAGAAGAATTTTAACTTATATTAGGTGTTTAAAACACCACAACTTATTTTTAATTTTGAATGATAAAAAAACAAGCAATTTGATTTTCAAATTGTTGGTTTTCTATTTATTTTTTTAATTTTTAAAATTAATCCCATTAATCAACATCCGAAAGATCTAAATAGATATATCCTTCCGATTCTTTACCCATTAAATTGTTTTTAATCATATCTGGACAAAGAATTTGTATATAAATACAAGGCATATTCTTTTCTTCACCTTTTTTTAAAAATTCAATTAAAAGTTTGTGACCTCAAGGCATAAGATTTTCAGGAATTTTTATATCAAAATATTTTTTAATGTCATTAGCTTCCATAAAATATGTATAAGCAATTCTTTCTTGACTTTTTAACATTTCAATAGTATCGTATTTTTTAGTTTCATCAATTGCTTTATTGACAATTTCAATTAAGGCTTTGGCTCCATTATTGGCTACAAATTTTTTGCCTTCTTTGGAAAGTTCAAATGAAAATTGTGTTGTTATTTCTGAAATTTGAGAAGCGGATAATAGCTTTCTTTGAAACATTCTATGAAGTCTATCGGGTTGTACGGGATCTGTATCATCAGTATTTTCGTTATTTGTATCTTTATTGTCATCTTTCTTGTCGTTAGTATCGGCGGTACCGGTATTTGTGCTGTCTGTATTTTTGTTATTAGTATCTGTAGTGCCAACACTAGAATTACCTATATTCTTATTATCAGTATTAGTGGTACCAGTATTTGTACTATCTGTATTTTTGTTATTAGTATCTGTAGTGCCACTATTATCTATATCAATAGTGTTATTACTTGTATCTAAGGTATTATTAATTGTGGTAGTTTCCTTGCAAGATATGCTTAAAAGAGGTAATAATGACACAGCCGGAAAAATAAAACTTAATAAAATATTTTTTTTCATATTCCCTTCACCTTTTAATAACTATGATTATACAACAATACGGCAAAATATATTAGTCAAAAAAATTTTAAATTTGAGCAAAATAATGTTTATATTTCTTTACATTTAGTAAAATTTAATTACTAGATAACAATATTTTGTTTTTTAGTTTAAATTTCAAATAATAAAATAATAATTAAAAATTAAATCAAGGAGAAATATGATTTCTAAAATTTATGATGATAAAAAATATCTTGAAAAAATGGATAAATGATTTAGAGCTGCTAATTATTTAGGTGTTTGTCAAATGTATTTAAGAGACAACCCTTTGCTAAAAAAGCCTTTAACATCAAATGATATTAAACTATATCCTATTGGACACTGAGGAACAGTTCCTGGACAAAACTTTATTTATACACATTTAAATAGAGTAATTAAGAAATATGACTTAAATATGTTTTATATTGAAGGCCCAGGACATGGTGGACAAGTTATGATTTCAAACTCTTATCTTGATGGATCTTATTCAGAAATATATCCTGAAATATCTCAAGATGAAGCTGGACTTGCAAAAATGTTCAAAAGATTCTCATTCCCTGGTGGAACTGCAAGCCATGCAGCACCCGAAACACCCGGATCAATTCATGAAGGTGGTGAATTAGGATATTCTATAAGCCATGGAACAGGAGCAATATTAGATAACCCTGATGTTATTTGTGCAGCTGTTGTTGGCGATGGTGAAGCAGAAACAGGCCCACTAGCAACTAGCTGATTCTCAAATGCTTTTATTAACCCTGTAAATGATGGGGCAATACTTCCTATATTACATTTGAATGGTGGAAAAATTTCAAACCCTACACTTCTTTCAAGAAAACCAAAAGAAGAAATTAAAAAATACTTTGAAGGTTTAGGCTGAAATCCAATTTTCGTTGAATGAAGCGAAGATAAAAGTAATCTTGACATGCATGAATTAATGGCAAAATCATTAGATAAGGCTATTGAATCAATTAAAGAAATTCAAGCAGAAGCAAGAAAAAAACCTGCTGAAGAAGCAACAAGACCAACTTGACCTATGATTGTTTTAAGAACTCCTAAGGGATGAACTGGACCAAAGCAATGAAACAATGAAGCTATTGAAGGAAGCTTTAGAGCTCACCAAGTTCCTATTCCTGTTTCAGCATTCAAGATGGAAAAAATTGCCGACTTAGAAAAATGATTAAAATCATATAAACCAGAAGAATTATTTGATGAAAATGGAACTATAATAAAAGAAATCAGAGATCTTGCGCCAGAAGGTCTAAAGAGAATGGCTGTTAATCCAATTACAAATGGTGGAATCGATAGCAATCCTTTAAAATTACAAGATTGAAAAAAATATGCCTTAAAAATTGACTATCCTGGTGAAATAAAAGCACAAGATATGGCAGAAATGGCAAAATTTGCTGCTGATATAATGAAAGATAACCCTTCATCATTTAGAGTATTTGGACCTGATGAAACTAAATCAAATCGTATGTTTGCATTATTCAATGTAACAAATAGACAATGACTAGAACCAGTTTCTAAGAAATACGATGAATGAATTTCACCTGCAGGTAGAATTATTGACTCACAATTAAGTGAACACCAATGTGAAGGTTTCCTAGAAGGTTACGTATTAACCGGAAGACATGGTTTCTTTGCATCATATGAAGCATTTTTAAGAGTTGTTGATTCAATGCTTACTCAACATATGAAATGAATTAAGAAAGCTTCAGAATTATCATGAAGAAAAACCTACCCTTCATTAAATATTATTGCAACTTCAAACGCTTTCCAACAAGACCACAATGGTTACACTCACCAAGACCCAGGACTTTTAGGACACTTAGCTGATAAAAGACCAGAAATTATTAGAGAATACTTACCAGCTGATACAAACAGTTTACTAGCAGTAATGAATAAAGCCTTAACCGAAAGAAATGTAATTAACTTAATTGTTGCTTCAAAACAACCAAGAGAACAATTCTTTACAGTTGAAGATGCTGAAGAATTACTAGAAAAAGGTTATAAAGTTGTTCCTTGAGCTTCAAATATTTCAGAAAATGAAGAACCAGATATCGTATTTGCTTCAAGTGGTGTTGAACCAAACATTGAATCATTGGCTGCTATTTCACTAATTAACCAAGAATATCCACATCTAAAAATAAGATATGTTTATGTTCTTGACTTATTAAAATTGAGATCTAGAAAAATAGATCCAAGAGGAATTAGCGATGAAGAATTTGATAAGGTATTTACTAAAAATAAACCTATCATATTTGCTTTCCATGGATTTGAAGGATTATTAAGAGACATCTTCTTTACAAGATCTAACCACAATTTAATTGCACACGGATATCGTGAAAACGGTGATATTACAACATCATTCGATATTAGACAATTAAGTGAAATGGATAGATATCATATTGCTAAAGATGCAGCAGAAGCAGTATATGGAAAAGATGCAAAAGCATTTATGAATAAATTAGACCAAAAACTAGAATATCATAGAAACTACATTGATGAATATGGATATGATATGCCAGAAGTTGTTGAATGAAAATGAAAGAACATAAACAAAGAAAATTAATTTTAGTTTAAATAATTTCTTATATTTATGGTAAAATTGACTAAGTTAATTAACGAAAAATAACATAGGAGTACACATGCAAAAAGATATTCACCCAAAATACAACCAAATTAAAGCTGTTTGCTCATCATGCAATTCAACAATAGAATTTGGTTCAACTGCTGATAAAGTTACTTTAGATGTTTGCTCTAATTGTCATGCTTTCTACACAGGAAACCGTACAGATGTTAAAGCTCGTGGACGTGTTGAAAGATTTAATAAGATATTAGAAAAATCACATAAAAAGAACTAATTAATTAGCATTAATAAATAGGCGAAATGCCTATTTTTTATTATGTACAATAATGCATACCAACTGCATAACCAATGTTTTCATTGCTATTGTCTCATTTCAATGAAATATTCCCGGCTTTTACTTCTCCTTTATTTGCATTTAAAGTCTTAGCCAAGCCTATTGAAGTTCCAATAATTCCAACAGTGCTTGTTACTACAGACAAAATAGTTGAAATTAAAGCAGTAATTGCAAAACCTCCTACAATATTTTTTGTTTCTTCTTCGCTCATTAGTTTGAAATTTATATTCATTTTTATTTCTCCCTTCAATTTCAAATATAACAAATATTCCATTATGCTGAATGTATTGAAGAAAAAATAGCGAAAAGTAACAATCTTTTTCGCTATTAGTAATTTAAAATTGTTATGCTCTTGTTATTCGCTAATATTGCTAATAAATCTAAAAATATTGCATATTTAATCTCGTTGAGTTTTCAATAAATGTTATATTCATTCTTTATCACAATTTTCTTTAGTAATACTTATTTCTTATTTGATAAACAAAATTTAAGGCCAATATTATTTATACACTATATTTAGAATTATTAGTTCTTCAATAATTATTATTGCATTGACTTTGATAAATAACTTTGTAAACTATTTTGTAACCTTTGCAGTATATTCACTACTAATATTAATATGTAGCATAACAATAATCTTTGCGAACGAAAAATTAATTTAAAAATTAAGCAAGTTAAAGAACAAAAAAATACAGTTTAAAATTCTATGAATAAAAAATTAGACTCATATAACTTAAGGGTTGAGAGTCTAATTTTTAAATTTGTATTTTTATTATTATTTTGTTGAATCAAAAGTATTTTCACTATCAATGTTTTTATCATTGGCTAGTTGTTCTTCTTTACTTTTATTTTGTTTATTATGAAAGCATAAGCCTATTATTATTGCAGAAATTATTGTTCCAATTCCTCCACAAATTAAAAATGAATCTGCGAAATTGAATGTTCCTTTATCAATTCATGGAAAATATATAATATCTCTTACACCTTGAAATTGAAATCTATCATACATATTTCCAAGCGAACCTGCTGCTGTAACTGCTAGTCCTGCAACTACAAAAATATATCAATGATTTTTAAAAAATAAACTACCAATGAAAGTTGCAAAAATTATAATAAAACTTATTATGTGTAATCCAACGTTGCCTAATCCTAATTCCAGGGTAGTTCCAGCATGGAATACAGTTCTATATCATAAAAATGCATGTGCTCCGGGTTGTCCAAATGAATAAATAGCTTCAGAATCCCTATAATCTGCCGGAATATAATAATTTCTTTTAGTTAATAAATCAATTAAAGCAAATATTATAAATATTCCTATATAAACAGAAGCATTTATTAAAGCATTTTTTCATTGGCTCTTCCAATATTCTTTAGTAAAAATGGAAATTTTTTTATTTTGCATCAATTACATCCTTGCATCTTAAACAAATTTGCAATTCATCATCATAATCTGAATCTTCAAAATGGTTTCAACATCTCAAACATTTTTTGGTTTCTAATTCTCTTACTTCGATGGTATTGCCAAACGTTACCTTTGCCACCATTAATAATCTATTTAATGGCAATGATTTTATAAAATCTGAATCGCTTTTTATTACTACTCATGCTTCATTTGGTCTCTTTATTGCTTGCGATTTAATTTTTTCTTCAATTAATTTGTATACTTCATCTTTTATTTCGAAGAATTCTTTTCATTGATCTTGTAATTTAAAATCGAAATCTTTTCTTTCTAAAAATGGCAATAGATGAACAGATGGCAACTTGTTAGGTTTATTGTAATATTTATAAATTTCTTCGGTTGTAGTTGGCAATATTGGGGCAAGAGCTTTTAATAAGACCTCAATAATGTTACTAAAAACATATTGAACCATTCTACGTTCAGGGTCATCTTGTTTTTCAAGATATAAAATATCTTTGGTTATAGAAATATAGTAACTACTTAAATCAATAACAAAATTATTAATATCTTTAATAACATTGACAAATCTATATTGTTCGTAATTCAATAATATATTTGCTTCCAGATTATGCAATTTTTCTAACATTAAAGCATGAATTGAAGTTGGCGTAATTTCTTTAAAATCAAAGTCACTAATTCCACCAAGCATGAACTTAATTGTATTTCTAACTTTACGGTAAATTTCAGTATTTTGGTTTAATATCTTATCATCAATTGTAACATCAGCAGTATATTCAGAATTTGCTGCTCAAAGTCTTAAAATATCTGCCCCATATTTATCAATCACAGCCAAAGGATCAACAACATTGCCTTTTGATTTTGACATTTTTTGACCTTTGCCATCTAAAACAAACCCATGAGAAACCAAAGCTTTAAATGGGCTAGTATTTCTTCATGCCACTGAGTTAATTAATGAACTATTGAATCAACCACGATATTGATCTGAACCTTCTAAATAAAGATCAAATGGAGCTTTTATTCCGCCTGGTTCAACACTTATTGAAGTTGAGCCCGAGTCAAATCAAACATCCATAATGTCTGTTTCTTTTTTAAGACCTAAATTTCTAAATTTTTCTGGCAATAGTTCATCAGCTGTTCATTCGTATCAAACATCGCTACCATGTTCTTTAACTAAATTAATTACATGTTCAAAAATTTCATCATCTAGAACAGGATTTTTGTTTTTATCATAAAAAATAATTATTGGAACTCCCCAAGATCTTTGACGAGAAATGCATCAACTTTCACGATTTTCCAACATTAGACTTAAACGTTTTTTGCTTCATTCATTGAATGTTTTAACTTCACTTAAAGCCTTAATGATTTGTTGTTTTATTGGTTTTAATGAAACAAATCATTGTGGTGTTGCTCTATACATAATTGGTAAATGAGTTCTTCAATCGTGAGGATATGAGTGTTTAATTTTCTTAAACGCAATTAATTGATTATTTTCTGCTAAAAATTTGCCAATTAAAGGATTTGCATCATCGTAAAAAACACCGCTATATTCAAAGCCTTTATCATTGATTGTTCCATCATCGTTTATGTGCATAATCATTTCTAGGTTTTCTTTTTTGCCGATAATAAAGTCATCTTCACCAAATAATGGAGCAATGTGAACTAAGCCAGTACCAGTATCTAAACTAACATGATGACCAAAAACAACTGGCCCCACTAATGGGGTTATAGGATTTTTATATTTAATTCCTAAAAATTCTTTACCTTTGAATGTTGATAGAACTTGATATTCTTCTCATTTAGCAACTGCAGCAACTGATTCAACTAAATCTTTTGCAATTATATATTTATTGTCATTTACTAACACTTTTGCATATTCGAATTGATCGTTAATTGCAACTCCAGAGTTAGCCAACAATGTTCATGGGGTTGTTGTTCAGATAATTAAATAATCTCCATTTTCAACACATTTGTTTCCTTCGACTACTTTGAATGCAACATATAGAGAAGGTGAAATGTGATCAGCATATTCGACTTCTGCTTCAGCGAGTGCAGATTGAGAAGAAGGTGATCAATAAACTGGTTTTAAATCACGATAAATTAAGCCTTCTTTAACCATTTTAGCAAAAAGTTCTAATTGTTTAAATTCTAAGTTTTTATCCAATGTCACATAAATTTCTGAAAAGTCTGTCAATAAACTTAATTTTTTAAATTGAGCCTTTTGATGTTCAACTTGACTTAAAGCATATTCTGCTGCCTTTTTTCTTAATTCAATAACACTAAAATCTTTAGCATTCTTTTTAGCTTCTAGCAACATTTTATGTTCAATGGGCAAACCATGTGTATCTCAACCCGGAACATATGGAGAATAATATCCCTGCATACTTCTAAATCTTACAATTATATCTTTTAGAATTTTGTTTAGAGCGTGTCCAACATGAATATTGCCATTTGCATATGGCGGACCGTCATGTAATACAAATGATTGACTGTTCTTATTTCTATTTAAAATAGTTTGATACAACTTAATATCTAGCCATTGTTTTTGATATTCCAATTCTTTTTCAACTAAATTTGCTTTCATTGGAAAATCAGTATTTGGCATCAATAACGTGTCTTTATAATCTTTCTTTTCTTCCATTATTAACAAGCCTTTCTTTTTCTATTTAATTATGAAATTTAAAATTTTAAAATCTTTATATATTACTTTAACTATTTCATGACCTTGTAATCATTTTGCAACATTGTCTAGTTTTTTTGCATTTTCAATAATTTCTTCATTGCTTCAATTTGGCAATATTTCAATTTGAGAACGAACTTTTCCGTTAATTTGAATACCAATTTGTATATTTTTAATAATTATCTTGGTTTCATCAGCATAAGGTCAAGTTTGATATTGAATTGATTTTTCATTCAACATAAACAAAAGTTCTTCAGCTAAAAATGGCGCAAATGGACTTAATAAAATAGCAAATTGTTTTAAAACAAATGATGATGGAATAGTTTTTAAAGATGTTAAGTAATTAGTAAAGACCATCATTTTGCTTATTGCAATATTAAACTTAGTTTTTTCAATATTTTCGCTAACTTCTAAAATCAATTTATTAATGTTGCTTATCAATTCATTATTGCTATTGTTTATATCAATATTTCCTTTGACAAAATATTCAAATATATTGTAGATTTTTTCAATCCATCTTCTTATGCCATTTAATGAATCTGTACTTCAAGATTTTGATTCTACCAAAGGCCCCATAAACATTTCATAAACTCTTAAACTATCCGCTCCATGAGATAAAATAAAATCATCAGGATTTATAACATTACCTAGAGATTTAGACATTTTTTGACCATCAGGTCCCATAATTAATCCTTGGTTTATTAGCTTTGTAAATGGTTCTTTTGTATTAACAATACCCAAATCAAATAAAAATCTATGTCAAAATCTTGCATACAATAAATGCAAAACAGCATGTTCTTGCCCACCAATATACAAATCAACCGGCATTCATTCTTCAAAACGCTTTTTTGCTTCCGATGAATTTAGTGGAGTATATGAGCCATCTGGGTTTTTTAATATATAAGCCAAATAATATCAACTTGAGCCAGCCCATTGTGGCATAACATTTGTGTCATGATGATATTTTTTACCATTAATTTCAACATTCATTCAGCTTTCAACATTTGCCAGTGGACCAGAGGTTGTACCTGATGGTTTTATGTTTTTTATCTTTGGCAATTCAACTAATTCTTTTACTAGATAAATATTATTTTCTTCATCAAATAAAACAGGAAATGGTTCTCCCCAATATCTTTGCCTTGAAAAAATTCAATCGCGTAATTTATAACTTACTTTTTCATCTGCCAAATTATTTGCTTTCAATTCATTAAAAATACATTTTACTGCCCCTGCATTATCTAAACCATTTGCAATATCTGAATTGATATGAATACCATCTCCTAAAAATGGTAATTCATCACATTCAATAATTTTTTTAATTTTTAAATCAAATTTTTTAGCAAATTCATAATCTCTTTGATCATGGCCGGGCACACACATTACTGCTCCACTTCCATATGACATCAAAACGTAATCAGAAACTCAAATAGGAATTAATTCATTAGTCAATGGATGAATTGCATACGAGCCAGTAAAATATCCTGTTTTGTCTTTATTTAAAGATGTTCTTTCCAATTCATTTTTTAATTTAACTTCTTTTAAATAGTTAGAAATATCATCATGATATTTTGCGGTAGTTATTTTTTGAAGAAGTGGGTGTTCGGGAGAAATTACTATTGCAGTTACACCATAGATAGTATCCAATCTAGTAGTAAATGTTGAGAGTTGCAATTTTTGTTGTTCACTCGAACTAATATTTCACTTAACGATATGTCCCTTACTTCTTCCAATTCAATTTCTTTGCAATTGTTTTAAACTTTCTGGTCAATCTAAATCTTCTAGGCCTTCAAGTAATTTATCTGCATAGGCTGTTATTTTTAACACTCATTGTTTCATAGGCTTCTTAACGACAGGAAAATTACCCCGTTCGCTAACTTTAATACCGTTTTTTTCTAAAATTTCTTCATTAGCTAAAACAGTGCCTAGCCCTTCGCATCAATTAACATCAATTTCTTCAATTGAAGCCAGTCCTTGTTTGTACATTTCTTTGAATATTCATTGAGTTCAAATATAAAATTTAGGATCAGTAGTATTTACTTCCTTGTCATAATCATAAGAAAATCCCATTGCTTGCAATTGTCTTCTAAAATTAGCAATATTTTTTAATGTAAAAGGTTCAGGATCATTCCCTGTTTTTATTGCGTATTGTTCAGCAGGTAAGCCAAAAGCATCTCAGCCAATAGGATGCAACACATCAAAACCATTTAGGCGTTTATACCTAGCAATAATATCAGTAGCGGTATAACCCTCTAAATGCCCAACGTGCAATCCCATTCCCGAAGGGTAAGGAAACATGTCAAGTATATAGGCTTTTTTGTTGCTATTATTTCCTGCGGCAAACGATTTATTTTCGGCTCAAAATTTTCTTCATTTTTGTTCTATTTCATTGTGCTTATACATAATATTTAAATTTTACATTAATATTAATTAAAAAAATAAGAAATAAAAAAAGTTGCGAAAATTTAATTGATCTAAAAAAATAATTAATTTAAAAATAAGCCAATGCATTGAAATTTCTAATTAATATGTTTAAAATTTTTCAATATTTAAAAAATATTACAAATTTTTATATCTATCCTGTAAAATTTACTTATTTAAAGACTAAAAACAAAATAAAGTTAAAAAAGTTTTAGTTTTATTAATAATAGAAATAAATTTAATCAAAGAGGTATAAACATGAAAAGAAAAATTTTACTATTTACCCCCTTAATTATTGCTGGTAGCTTAATACCAACAACATTGACAGCATGCAATAATGAAGATAGCAAACTTACAAAGCAATTAAAAGCAAAGCAAACATTTAATAATTTAAAGAATAAATTGCAAGATTTATTAAACTCAGAAGATGCAAAAGATATTGATGCAAATAAAGAAATTAATACACTTGCAAACACAACAATTGATGATAATTCTCAAATACAAGACATTGAAAATAAAATCAAAACTATTGAAGATGCTATTGAATCATTGACTAAGAAAATTAATGATAAAAAAATCCTAATAAAACTAAGACAAGATTTCAACAAAGCAAAAGAAGATCTAAGAAATATTTTGACATCAAAAGAAGTGGCGGATATTGAAAAAGAAATCGAATTTAACACTCTAAATACTACTGATATTGCCAAAGATTCTTTGCAACAAGAAATTCAAGCCAAAATACAAAAAATTCAAGAAGCTATACAATCAATAAACAAAAAAATTAGCGACAAAAAGCAATTAAACAAAGAACAACAAAATCTTGTTGAAGCAAAAAATACACTTGAAAATCTTATTGCTACAGAAGATGCAAAAACAGTTGGAATTGGTGAAGCAAAAAAAATTCTTGATGATAATAAGAATAATAAAATATCAACTATTGAAGAAATTAAAAAAGCAATAAATGATTTGAATGATATTACAAACAAATTAAATCAAAAAATTGCAAACAAAAAGCAAGAATTATTAGAAAATTTCAAAAAATCTAAACAAGATTTAGAAGAATACATAAATAAAAATTTAAATGAAATCAGATTTATTGAAGTTAAAGAAAAAGCTCAAAAAACCATTGAAAAATTTTCTAATATAAACAAAGATAATTCCTCAATCAAAGAAATTACAGATGCTACTATCTCACTAAATAAAGCTAAAGAAGTTGCTTTGAAAGAAGAGGAAGAAATCAAAAAATTAAACGAAGTCTTGAAATATGTCAATAAAAGAAAAGAACTAGAAGAATTAATAAAATCATCAAATATTCAATCAAATAATGATGCTATTAAAAAAGCTCAAAAAACTTTAGACGATAATAAAATAGCAAATACCAACGAATCATCTTCAAAAGATTTTGAAGAAAAAAGAAAAAATATTGAAAAAGCAATAATTGAACTAAAGGCAGCAATAGAAGCAACAAAAAAATCTAAGAATTAAAGATCAATATGTATTGATGTATTCATTCAATGTAATTAAACCCAAAATAATTTAAGTTGTTGTAAATTATTTTGGGTTTTAATATTAAAATGATTTTTGTACATTATATTTTATTAAAAAAATATATATGCCGACATTAAAAAGCAATTAATTGCATAAAAAATATAAAAAATAAGCCATTTTTTCAACCAATTATATTAAAAATCTGTTTTTTTTTTTTTTTTTATTAAGATGTTTTACTTTTTAATATATATAAAAATAAAATTATAAATAATAATGAAATGTTAAAATTACAAATATAAATTAATTTATTTTAAAAATTATGGAGTGTAAAAATCTATGGGTAAAAAAGCAAAATTATTTGTCTTTGGTGGGATTAGTTTTTTAGCAGGAGCTTCAATTATTGGTTCTGTTGCAGCATATGCCTCAATTAATAAAAAGAATGAAATTAGAGTTAGCGAATATAATAAATTTCTTGAAGAAAAAACTAATTTAAATTCTTTTAGCAATTTAGAAAATGATAAAAAAATTAGTTCAATAAAATCTAATACATTAAGTGAAGTTGAAAAGATAGATAAAAATTCAGATTTATTTACTATTCAAAAAGCCCGCAAAATAGTCAACGAATCCATTGAAAATATCAAAAATATAATAGCATCGCATAAAAAAATGTTATTAGAAAAAGAAAAAGCGTTTATTCAATATAACAATGTAAAAAAGCAATTGCAAGATATGCTTTTAAAAATTTTTGACTTTTTAAATAAAGAAAGCCAAGAACATTATTACATTGGCGATGGAAACGAAAAAATAATAAACAAACAAAATTGACTTTTAAAAGAAATAAATGAAAATCAAAAATATTTTTCAAACATAACGATTGATTCGGATATAGATAGCATAGAATTAGCAACAACTAAACTGGTTGAAATAAAATTTTTAATTAATGATAAACAAGAAGCATATAAATTTGCCAAAGAATATAACGACTTATTAAAAGAAATCAAGCAATTTATTTCAGATTGATATGAATTATTAAAAGATCCTGATATAAAAAAACACGAAGGTCCTTATGGTTACAACACGCCAGAAACTTTATTCGTATATTTTTTATTTAAATACAAAAATGATGACAAAAAAATATTTACAAAATTTAAAAATTATGCACAAGAAATGTATGACAAAACTGCACTTCTTGAGGAATTCGACATTGATTTTTTTGGATTCAAAATCAAAGATAAAAATTTCTATAAAAATTATGTTGAGTTGTTGACACCATATAAAAAGGCTATTGATTTGTGAAAAAATTATTATGATTCATATTCTATAGGGCTAAAAGAAGTAGAAGAAATTATTTGAGACAAATTGTATATTAATGGTTATACTATCTTTTTTAAACCTAAAAAAATATATGCTTTGCAAAATCAACCTGAACTAGACAAAGAACTAACTGAAACTACTGATGATTATAAATTTAAACTAATGCTTGTTCCAAAAGAAAATACTATAGAACATAATAAATTAATAGAAAATTTTAAAAACTATTGCGACGATTTAATGAATAGAAGCAAAAAATTAAAAAACGACAATCAATAAAGGGGGGCACGAAAATGAAAAAAAGAACAAAAATTGTAACCTCATTAAGTCTTTGCTTAACTACCGCAATTTCAACAATAGCAATCTATTCAATAATCGCCGTTAAAAAAAATATAGAACTTGATAAGTCAAATAAATATTCATTATTTATACAAGAAAAATCTAAATTGCAATCGCTTATCTCAAATAATCAACACAAAATTCCAAATAGCTATTTAAATTCAATTCAATATTTTCATAATATAACTAAAACCTCAAAAGCAATAGAAATAATTGATGCAACATCAAAAATAAGAAATGAAATTATTGACGTAAATGAAAAAATTCTTGCCTTTGATGTAGAAATAAAAAATAAACTATCGCTATTCAATGAATTTAATCAAATAAAACAAAAGCTTGAAAATGATTACCATAACAGACTATATAAAATATGAAGAGCAGATGCTGGAGAACCAGATATGATAGTTGATTTTGGTTTTCTTGAAGAAGAAACTCATTTTCAAAAATATCTTGAAACAATTAAAAAATATTCTTGAATTTCTAAAAATTCATCATCATTTGAAATAAAAAGGGCTATCTTTGATTTGAAAAATATTGAAAATAATATTGATTATTTTTTAGTTAAAAAAGAAGTTGAACATTTATTTGAAAAAATTGAAAGCACTATTTCAATAGTTGAAACTAGACTAGAAAAAAATAAAATCAATAAAATATTTGAGGATAATGAAGTAAAAGAAATTTTAGATTTAATTTCACAGTTTAAATTAGATTTACAAAAACAAAAAGATTTATATAACTCAGTAGACAATAAAACCGATAACATCAAACACGAAACTTTTAAAAAAATATTTAGCCATTATAATAAAAACAATTTCTTTAAAATTTTTGATTGCATAGATCAAAGAGTTGATTTATATTACGACTTTATGTTCTTTTACTCACCTTTATATTTTTATGAACCAACAGAAGAAGTCAAAAAAGTTTTCCAATATCCTGATAAAAAAGATTTTGAAAAAGAAATAATTTTAAAATATAATCAAGCCAAACAAGATCTTACTAACTTCAAATCAAAGATAAATGGAAATGAAAAAATTGCCAAAAAAGCATTCAATAAATTCAATGAATATATACGCAATTTAGCAATAAGTTTTGAAAAGGAATTAATACAAAAAAAATTATTATTATTAAAAAATTTATTAACCAAATATGATCAAAAGCAAGATGGAAAATCACAATTCATAACAGTTGAAATAAAAAAAGCCATAAATGATGCAGAATCTTTATTAAGTTATAATGACTTAAAATATTCCACAAGAGATAAAAAGAGTATTTGAGAATTTAGAACAGTAAGAAATAATTTGCAAGAAATAATAGACAAATATTAAAAATCCTTGAATTGTCCTACCCTTGTAATAATCAAAAGATTATTCAAACACAATACTAAAAACAAGATAACTGAAAATCATTATCTTGTTTTTATTTTTAATTTAGCAATATTGAAATTAAATTTAATTGACTTTTTGTCTAATTAAATGTAATTTATTTTGTAATTTAAGTAATATTTCTGTTAAATATTTTAAATTTGGCTGCAATTTCTTAATATAAAAATCTTCTAATTTTTCTTCGTGTTCTTCTATAAGTTTTCTAACTTCTTGTATTTCGTTGGAAATTTTTTGGTTTTTAGAATAGACATCATTTGCAAGAATATTAAATTCCTCAACATTTGATGATATCAATTTCAATAAATTTCACCTATTTAAAATAATTTCTAAGTTTAATTTTCTAGAAAGTTCGTATGTAATTTCCATATTTTTATTGATTTTGGCAATATCTTTTGAAGAAAATAGCTCAGAATTTTCTTTTAATATTTTTGCTATTTCATTATTTAGAAATTTACTTAAAAAATTTCCATCTTCTGAATTTTTTTGTTCTTTTAAACTTTCATTAAGCATTTTTAAAAGTTTAATGCTTTCTTCTTTATTAGTAATGTTTAAAAAATCCTTATTTATATTTTTATAAATTTCTTCTTGGACAGTTAGAATTTTTTTAAATTCTTCATGTATAGATTTTTTTATTTGTTGATCATTTCTATTATTTTCCAATTTATTAATTGTTGCTAAAAAATCTGTAATTTTATTTTTTGTTTCTTGACTAAATAAATTAAATAAATCGCTATTTTTTTGTTGCATTTCAAATTCATTTTTTGCATTATAAATAATTTGTGAATATAATTCAATATATTCGGCCAATAATTTATTTGATTCTTCAAATTGATAAATAACTTCTGCAAATGTTTTTGAATCAATATCCTTGAAATCATTAAATAATTCGTTCAACATTAGCCTGTCCACAACATCTCATGTTTTACTATTTTTAAAATTATTAAAGTTAATCAATTCTTGTATAAGCGGATTTAAATCATTTTTAACATTATTTAAATGTTTTTGTATATCTTTTGATGAATTTATATTTTGATAAATGTCTTCAATTCTTTTTGAAATTTCACTAGTTAATTTCGCTATATCTTCATTTTTGAAATCAAAATTAACATTATTAATCTTTTTAAGAAGTTCTATTTCCTTGCTCATATCTGCTTTGTTTCATTTTTTATAAATACTAAATAGATTTTCTAATTTTCTTTTTTGACTCAAAAATTCAACATCAATTTTTGATTTTGGACTTAGTGACTCTTTAGTATATCTTTGGTCAATTAAATTCTTTTGAACGCTAATGTTTCAAAGAATTTCTTTAGATCTCAAAATAAATTCATCAGCCTCATTTTTTTCTTTTTCAATAAAATCTTTAATTTCTAATTTTAAATTTTCATAAATAAAATCTTTAAGATTATTGGCTATATATGTTTGCAGTTCATTTTTAACTTCTTGTAGCCTACTAGGACTAGGACTTGCCGCATTATTTTTATCTTCTTTTGTTTTTTGAGATTCATTTGGAACAGTTGGCTTTATATTAGGATTAATTATGTGGCTATTATCTTCTTGTTTTTCATTAGATATATTTGGCTTGACATTAGGTGTATTTGCTACATTGTTGTTTGAATCTTCTTTTTTATCTTGTTTATTAGAACATGAAGCACTAGAAATTACTGTAAATACAGTTGCAAACGATCCTAAGATAGTTGATAGAATAAATAGTTTGTTTTTTTGTTTCATATGTGTTTCCTTTGATTTGATATTAATAAATTATTTTTTAATAATTATTAATATAATATAGAAATATCAAAAACTTCTATTTAATTTAATCAATTTTTTAAAAATCAATAAATTATTCTATTAAAAGTACAAAATTAGATTTGCTAACAAAAAATATATTTTTTTAACTTTTGTTTCTAATATATTATATAATTATTAAGCAATTTAAAGGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCATGTTGCCCTTTAGCCAAGTGGAAAGGCAGCGGTTTTTGGTACCGCCATCCGTTAGTTCGAATCTAACAAGGGCAGCCATTTCATTAAATTGTCCAAAAAAGCGCAATGCGCTTTTTTATTTTATTAAAAACCTTCCGATTTTTAAGTTTTACTATCGGAAGGTTTTTAATAAATTATTTATATTATTTGTTAATGTTATATTTATTTTTATATTCAATAAATTTGGCTTGTATTTCAGCTTCTTTATTTTTTAGTTCTAAATTGCTATGTTTTCCTGAATTCTTCAAAATTTCTTTAGCTTCTTGTATTAATTTGTTTAAAGCATCGTAGCCTTCTTTATATTCAGCTTTTCCTTTAAGCTTATTTTCAATAAAAGAAGATGTAGTATTTTTGGTGTTACTCAATTTTTTTCAAAAATCAAAAAATTCAACAATAATTTCATTCATCTTATCTTCAATTGGTTTTTTTAATGCTCTAAAATCAGATTTAAATTTTTCTAATTTATCTTCCTGTGGTTCTTGTTTTCACAAATCAATAGCATCCTCTCTTATCTTACTAATTTCTTTTTCTGCTTCTTCTCAATATTTTTTGCTATCCAAACAGTTTGAAGAATCTGTTCGCATATCTCCTGTAAATTTAGTTACTTCTTTATTTACAAAACAATATATATCGCTACTAAATTTATCTTTCTTTGCCTTGTTTAGGTCTTTGGTTAATTGTTGCTTGGCCCCAGATATTTGTTGAATGTCAGAAGCAGAAGTAACATTTTTAAAGAACTCAAGTTGTCTGTTTAAATGGTCTATTATATTACTATCTCAATCATAATATTGTTGATTTTTATATTTTGTATAATATTCATCCTCTTTTACTTTTTCGATAAATTGTTTAATTTGTTCCCTGATTTTTTCAAGTCCTTCATATTCAATATTTTCCTTTGGCTTTTTATCATTAATTTTCTTAGTCAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAAAGTCTTTAATTAAATCATTACCAGAAAGATTTGTGTTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTGGCTTCATCTGATTTAATCAAATTGTCTAGTTGTTGTTTCTTTGAATTAAAGTCTTGTTTAGCTTGTTCTTTAGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATGAACAATTTGATCGGTCGTTGATGAAGTATCTGCATTATTGTTTTGCAATGATTGATTTGCTTTAGATGTGTCGACTTTTTGACCGTCTTGTGAATTAATTAAGTCTTCTAATTGCTTTTTAGCGTCATTAAAGTCTTTAAGAAGATTTTCTTTTTTATCATTAATTTTTTTGGTCAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGACTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAAGATGTTGGTTTCTTCTTGTTTATCAACATCTTTGGCTTCATCTGATTTAATCAAATTGTCTAGTTGTTGTTTCTTTGAATTAAAGTCTTGTTTAGCTTGTTCTTTAGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAACAATTTGATCGGTCGTTGATGAAGTATCTGCATTATTGTTTTGCAATGATTGATTTGCTTTAGATGTGTCAACTTTTTGACCGTCTTGTGAATTAATTAAGTCTTCTAATTGCTTTTTAGCATCATTAAAATCTTTAAGAAGATTGTCTTTTTTATCATTAATTTTCTTAGTTAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAAGATGTTGGTTTCTTCTTGTTTATCAACATCTTTGGCTTCATTTGAATTAATTAAATTATCTAATTGTTGTTTCTTTGAACTAAATTCTTGTTTAGCATTTTCTTTGGCTTCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAACAATTTGATCGGTCGTTGATGAAGTATCTGCATTATTGTTTTGCAATGATTGATTTGCTTTAGATGTGTCAACTTTTTGACCGTCTTGTGAATTAATTAAATCTTGTAATTTCTTTTTAGCGTCGTTAAAGTCTTTAAGAAGATTGTCTTTTTTATCATTAATTTTCTTAGTCAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGAAAGATTTGTGTTGTTAAAGATATCAGTTTCTGTTTGTTTATCAACATCTTTGGCTTCATTTGAATTAATTAAATCATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTTTGGCATTTTCTTTGGCTTCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAACAATTTGATCGGTCGTTGATGAAGTATCTGCATTATTGTTTTGCAATGATTGATTTGCTTTAGATGTGTCGACTTTTTGTGCGTCTTGTGAATTAATTAAGTCTTCTAATTGCTTTTTAGCATCATTAAAATCTTTAAGAAGATTGTCTTTTTTATCATTAATTTTCTTAGTTAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAATATGTCAGTTTCTGGTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATTATCTAATTGTTGCTTCTTTGAACTAAATTCTTGTTTAGCATTTTCTTTGGCTTCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAACAATTTGATCGGTCGTTGATGAAGTATCTGCATTATTGTTTTGCAATGATTGATTTGCTTTAGATGTGTCGACTTTTTGTGCGTCTTGTGAATTAATTAAGTCTTCTAATTGCTTTTTAGCATCATTAAAATCTTTAAGAAGATTGTCTTTTTTATCATTAATTTTCTTAGTTAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTTGCTTCATTTGAATTAATTAAATTATCTAATTGTTGTTTCTTTGAATTAAATTCTTGTGTGGCTTTTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATGAACAATTTGATCGACTGTTGATGAAGCATCTACATTGTTGTTTTGCAATGATTGATTTGCTTTGGAAGTGTCGACTTTTTGTGCGTCTTGTGAATTAATTAAGTCTTCTAATTGCTTTTTAGCATCATTAAAATCTTTAAGAAGATTGTCTTTTTTATCATTAATTTTCTTAGTCAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAAGATGTTGGTTTCTGGTTGTTTATCAACATCATTGGCATCATTTGATTTAATTAATTTATCTAGTTGTTGCTTCTTTGAATTAAAGTCTTGTTTAGCTTGTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAACAATTTGATCGCTCGTTGATGAAGCATCTACATTGTTGTTTTGCAATGATTGATTTGCTTTAGATGTGTCAACTTTTTGACCGTCTTGTGAATCAATTAAATCCTTTAATTGCTTTTTAGCGTCGTTAAAATCTTTAAGAAGGTTGTCTTTTTTATCATTAATTTTCTTAGTTAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAAGATGTTGGTTTCTTCTTGTTTATCAACATCATTGGCATCATTTGATTTAATTAATTTATCTAGTTGTTGTTTCTTTGAATTAAAGTCTTGTTTAGCTTGTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAACAATTTGATCGACTGTTGATGAAGCATCTACATTGTTATTTTGCAATGATTGATTTGCTTTAGAAGTGTCAACTTTTTGGCCGTCTTGTGAATTAATTAAATCTTGCAATTTCTTTTTAGCATCGTTAAAATCTTTAAGAAGGTTGTCTTTTTGGTTCTTTTTATCATTAATTTTCTTAGTTAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTGAATCTTTATCAGTAAGATTTGTATTGTTAAAGATGTTGGTTTCTGGTTGTTTATCAACATCATTGGCTTCATTTGATTTAATTAAATTATCTAATTTAGATTTTGATCTATTAAATTTTTCCTTTAATTTGTCAATATTATCTTCTTGTTCATATTGTCTTCTTTTAAATTTACTTTTTAGTGATGTTGCAACTAAAGTTGCTGTTGTTGCTAAAGCAATAATTAACGAAGTAGAAAGTAAAATTCTTAATTTCGATTTTTTCATATATCCCCTTTTTTTATTTTTCCCTTATTTTTTATATAAAAACTTATAAACAATTATTAAATTCTTATATTGTGATAATTTTCAAACAAGACTTCGAAAAAAAAAAAAAAAACGGTTTTCGCTCTATTACAATTTTTTTATGCACCTTTTAGTTTATTTTAATTGTTTTCTATCAAAACATGCTTAAAATAAACTAAAAAATCAAACTCATATTTTTTAGTTGAGTTTGATTTTTATTTGTATTTGGTTACATTATTAATCTTGTTGAATATCTTTATTTGAACCTATATAGGCAAAAATTTCAGGATTTTGTTTTAAGTATCTTCCTTCTAATATTCTTATAACGCTTTCAACTGTTTCGTTTAATGGTACAAATTGACCGGGCGATTTAGTAAAGTGTTCAGTCATAAAGAAATTTTGGGTAAAAAACATTTCGAGTTGAAGAGCTTTTTTAACAGTGATTTTGCTTTCTTCATCAAGTTCATCAAATCCCAATATTAGAATAACATCTTCAAGATCTTTATATGCTTTTAATATTCTTTTTGCTTCAATTATTGCATCATAGTGGCGTTTGCCAATAATGGTCGGATTAATTGCGTTTGTAGATGAAGCAAGTGGATCAAAGGCAGGAAAAATATTTTTAGCTGCTTGAGATCTAGATAAAACTAAATTACCATCTAAGTGATTAAATACAGCTACTGCAGAAGGGTCGCTTAAATCATCCATTGGTAGAAATATTGTTTGGAATGAAGTTATTGAGCCCTTCTTGTTTCTAAACAATCTGCTTTCAACTTCAGTTACATCACTTTCTAAAGTTGATTGATAACCTCCAATTGAAGGCTTTTTGCCTAATGCTGAACTTACTTCATTTTCTGCTTGAATAAAACGATAAATATTGTCAATAAATAATAAAACATTTTCATTGCATGTGTCTCTTAAATATTCTGCAGCAGTAATTCCGATTGGAACAATTGACGTTCTAGCTCCTGGCGCTTCATTCATTTTTGAAATAAACATTATTGAGTCCTTCATTAAATTAGATGAAGAAAGTTCATTGTATAATTCTATTCCTTCTCTTGAACGTTCACCTGAACCAATAAAGATATTAGAATTTGCTTCATGTTGTTTATTAAGGTTAAAAATAATTTCCTTCATTAGAACAGTTTTTCCAACTCCGGCTCCTCCGAATAAACCAATTTTTGCGCCCTTAACGATTGGCATAAAGAAGTCAATTGCCTTTATTCCGGTTTCTATAACTTCTTTGTTATTTTCTAGATATCTTTCATTGTTTATAGTCGAATTCATTTCTACATATTCAATTTTATTTTTTGTTTCTAGTAGAGGTTGACCTCAGAAGTTGTAAATATTGTTTTTTGAATTTTGGCCTATTGGAACCAAAAAACTCTTATGAGTGTTAACAACTTCTTCGTTAATTGAAATATCTTTACTTGCATATACAACAATTGCTCTTACATGTTTTTCGTCAATTAATCTTTTTACTAATAAAAAAGTTTTCCCTTCGTGCAATGTCAATAAATGATTGATAGCAGGTAAATCTTGATTAAATTCTACTTCAACTATATCTGATCAAATTTTTATAATTTTTCCATTCATTATTTATCACCTAAAACCTTCGCTGCCTTATCTAATTGTTCTTCAGGTATTGGCACAAAATTATGTTCTAATTCAATATCCCAATTTAAATTCAAATAATCTGAATATGATTTCAAGACAAATGCAAAATAATTCTTCATAATATTATCATCGTAGTCTAAATTATCTAAAATTGTTTTAAATGCTTTTATGCAATCATTATAATTATTTATTAAATAATCTAGTAACATAAATGCTTTTTGTTCATCTTTTATTCCTTGCAAAATACCTCAAGAAATTAATTTTGTTGTTAGCACTACAAAACGATATGAGTAAAGTGAAAAACCCTTTTGCAAGAACATTTTGTCAATTAATTTTCCTTTGTGAAGCAAAATAGAAGTTTCTTTATTAAGCTCGTAATCTAACATTGCCAATTTTAAATGTCTCTTGTAACTTCTAAAAATTTTATTAATTTCACCAGCTATTTTTGTCATCATTCTTGATTGAACACTTGAACCTGTTCTTGATACTGATAAATCAATGTTTATAGCAGGTAATTTCCCTGAAGCAAATAAGTCAGAAGAAGTTACAATTTGACCATCAGTTATTGAAATTATATTAGATGAAATTAAACTAGTTATATCACCGTCAACTGTTTGCAAAATTGGAAGTGCAGTAATTGTTTTTCTATTAACAAAAGATCCAGCTCTTTCAAGCAATGATGAGTGGGCAAAAAAGATGTCACTAGGCATTGCTTCTTTTCCAACTGACTTGTTGGTTAATAGGGCAATTTCTCTAATAATATTAGCATGTTTAGTTAAATCATCAAATACTATTAAAACATCATTGTCATATGAAAGATTTTCTGCATGGGCCATTCCGATGTAAGGCGCTAGATATTGTTCATAAACGTTTTGAGGCGCATCAATAATCATAGTGTTTTTCAATGCATCGTATTTTTTTAATGTTTCATAAATATTAGTTAATGATTCACGCTTTTGGCCAATTGCAACATAAATACATTTTGTGCCATTTTTTGCTTGATTTATAATTGCATTTAATGCCATATGAGTTTTTCCTGTTTGCCTATCGCCAATAATTAGTTCACGTTGACCTTTGCCGATTGGAATTAATAAATCTATTGCAATAATTCCGGTATAAAGTTGTTCATTTAATGTTTTAACTGTCATCAAATTATGAGCTAGGCCAAATATTGGATGATGATTGCTCAACATTGAATTATTTGATCTATTTTTTTCATTAGTTGGTCAAATGACGCCACCATTAATATCAATAATTTTGCCCAAATAATCAAGACTAGTATTTATATTTTGGGATTCATTTAATTCAATTAATTCACTATTTATATCTAAAGATACGTGTTCACTATTAATCAACATAAATGCCTTATTAGTTGTAGCAGAAATAACTATGAATTTAACATTTGGGTTACTTGCTAAATAAAATTGTTGATTTTGACGGTAGTTGTATTCACCCTCAATTGTTGCTATGCAATTGAATATTGATTTAATACGAGGATTTTTAGTTGTGTTGTTCATGTGTTCTCCTTCTAACTATATAAATAATAGCCCTCCAATTATTAATATTAGTAATGCGATCGCTAGTATTACACTATTGATAATAATTACCTTTTTCTTTAATTTTAAGTTTTTTAACAATGCAAAAATTATTATGTTTGTAATTATTATGATTAAAGAAATAACTATAAAAGAAGTCCCTAGTTTTTTAATAATCTTCTTTTGTTCAAGATAATTTTTATTTAAAACATTGACTGCTTCTAAATAAGCTTTATTGAAATTTTCTTTTTCTTCTTTATTAGCTTTTATTGAAATATTACTTGCTAAATTTGATAATAAGTCAAAATTATGTTTTATACCAGACAATAGTTCAAGGTAATCAGAATATCATTTTTCTTGGTTGAAATCTCTTTGGCTAATTAAAGCGGTAAATTTGTAGTTTAATTGTTTATTTAATAAGAATAATTCTCTTAAAACATTCTCATTGCCTTTGATTTCTGCTAAATTTGTTTTAATGAATTTTTCATTGAATTTAAGCGCTTCACTAAATTGCAATTGCACAAGATTATATGCCTTTGCTATTGTAGTTCAAAAGTATATATTATTTACTACAAGACTATTTAAATAAGAAAAAAATGTATAACGAATTGTTTTGCTAAACTCTTCAAGTAATTTTTTTGTATTTGTTTTATCTCCTTGCAATTTAACTCAATATTTTTGCAATATTTTATTGCTAATTGCACTATATGAATCTTTATCAGCTTTAAATGACATTTGTAGCGCTGAGTCAATCATTGCAAGCAATGCTTTTCTTAAATATCCATTTCTTAGCTTATTGTAATCTAACTTTTCATCTAAGCCTAAACCTTTGTATAATGGCTTAAATATTTCTGAAATTGATTTTATTTCAGTTTCTTTTAACAGATTAAAATATTTGTCTTGTGACGGAGTATATTTTTCAATTAAATATGTTCTTTCTAACTTAGGATTTATATTATCTGAAATATGTACTTTTAAATTAATAATATCATTGCCACTAATATCAAATGAAAAAACAGAATATTTATAGCGAGTTTTTATCGGGTTATTGAAATAAAATAATTCTTGACGTTTGCTTGCATCTAATGAATCAAATTGGCTTTTAATTTGACTTAATGAAGAAGGCAAATTGTTGCTTGATATAAACGGAACCAAAAGGGGCAATGATTGAATTAAATTTTTTTCTTGTTCTTTTGTTAAGTGGCTTGTTTTATCACCATCAACCAATGGTTTTAAATCGGGCAAAGAATCAGGATTTTTTTCTTCTTTTTGTTCAGCTTCGTTTGTTTCTTGATTTTGTTTTAAATCAAACTTTGTAATTCTTACTTTAAACTTTGAAATAAAATATTCATCTCAACTTTTAAAGCCTTTTGGCAAGGTGGTAGAAAATACTCCATTTTTATCCCTAATTAATTCGACATCTTTGTCAATATTTAAAACATCTTTTTCATTAAAAAATAACTTATTGACTTCTTTTAAGAAATCAGATTTATATTTCTTTAAAAATTGAGAATACTTATCTTTTTTAACAGTATTTAATTGATCTTTAGCAACTTCTTTAACACTTCCTTTTGGCAAAATTGCTTTTTTATAATCTAATGGATCGGATTTACCAGTTTTTATTGATTTAAATAAATCGCCTTCGTAATCAATATCCTGCAAATCATGGTTTTTTTCTTTACCTAATATTAGAGGAGTATTTAAATAAATTCCATACTTATTAGGATTTTTTTGAATCTCTTCTTTATTTTTTGTTAGAAATGTTTCTAAATTTTTTAAATAAATTAATTGTTGAATTTTTAATTTAAACTCTAATTCTTTATTTTCTAGCAATCTTTGTTGTTGAACTTGCAAATAATTGATTATTGCTTTTAAACCATCTTCTATTGTTGAAGAAATTGTTTTTTTAGCTTCACTTTCAAATTCACCAAAATTTGGATCTAACTCAGGTTTTTTATTATTTGGATCATTATTTGACGCAGCTGAAACTACTATTGGAGATAATAAAGGAACTGTTGCAAATGACAATAATAAGATTTTCTTTTTATTCATTGTTAATCCTATCTTTTTCAATTAGATTTAATTCTTGCCTCAAAGATAACAAATAATATTTTTCTTCAAGAACTTCTAATTTTAAAAATTCATCTAATTTTAAGCCTAATTGTTGTTTAGCCTTTCAAAATTTAATCTCGTTTGAAAGTTCAGATATTGTTTTAACTAAATTGACCTTGTTGAGCGATTTTATATAAAAATGAGGTTGGGTTAATGTTGAAATTGTAGCTATGTTATTGTTTACAACAATATTTGAATTTTTTAAAAAAACAAAGAAGGTTTCGTTTTGTTTTGAAATATCAATAATTTTTAGTAAAAGGTTTCCATAAGAAGCAATTGATGTATTTGCTATTTGTTCTCATGTATTTTCTTCATTGACATTAATATAAACTAAGCCTTTTTCAATATTTAGCTTCTTATTTTCTAAAAAAAGTAATTCAATTTTATATAAATTATTCATAAATATCACCTTCATCAGGGTCTTTTTTAGTCAATAAATTTAGTTCCTCAATTTGTAATTCACTTTTTGCTCTTGATATTTGCTTTTTAAGTTTAAGAATACTTTCGTCTAAATCTTTTAAAATTGTATTAGTTGAAACTAAACCTATTTTTGCTTTATAAAATGCAGATTCAGATATTAACATATTTATAGTGTTTAATAAATAACAATTAATTTGAGAATTAATAAAATCATTAATATTAGGATATATTTTTGCTTTTTTAAAATCTATGTCATATAAATTATTATTGCTTTCATTTAAAGGAAATTTTTCAAACGAGAATTTAGACATTGGCAAAATTGTAAAATATTGATTAAAATTTTTATTAGAATTAACTACAAAATTGACTTCTTCGTAATTTTCATATTCATACAAGATCATTACTATTTTGGTTAATTTTTCTGCCAAATTTTCAATATTTGAATTTTCATAAGATTGCAATACTTGTAGGTTATTATCTTGACCAAATTTAATTGCATTTTTGCCAATTAAAATGAACTCATTATTAGATTTTTTTATATTACTTAGAATAGTTTTTTCATATCTCGAATACGAGTCGGTGCTATATTTTTGTTCTTCTGTTAAATAAATTCACAATATGTTTCTATTTATTTTTCTAAATAAATTAAACCTATGGTGACTTTCTCTCCTCAACATATCATTCTTAATATTGTAGATTTCTTTTAAATGCAAAATTATTTGCCTTGATACCAATGCATTTTTATTAAAAAAGCTTAATCTTTGGTTAAGTTTCATAATATTTATTAAATAAATATTTTTTTCATTATTTACTTTTAATGAAATATTGTTTAAACTTTTGCGTTTATTCTCTAAATCTTTTAGTTGCATGTTATTGTTCCTTTGTAACAACTGCTACTACTTTTTCACTAAATAATAAAACTAAATCATTTTTTAATTCTGAATGAAATAAAATTACATGTGGAAATATCTTAATTGATTTTTTAAGGGCCAAAAGTTTTTTTATTTCAGCATTATATGAATTCAAAAATTCTTGGTATTTTATATCACTAGAAGCAGTTAAATCAATTGCGTTATTGGCAGAATAATTTTTTGTTGTAATTATAGGTACTAATACCTCTTGTGAGAATTTCAAATTCAAATTAAAAGTAATGTTAAAATCCTTTAGTAAAATGTCATATTTATTTTTAAAAGCCAAATCAATTTTGCTTATAAAATCTAAATAAATAGGAGAATTAAATCCTAGGTTGTTGTTAAATAATACTGTTGATATCATTTCATCAAAAGTAATTAAATCTTGTGAGGACTCGTGTTCTTTATATTCTTTAAATACATCAAAATAACTCAATAATGGGTTATTGTCTTTCTTTTTCATATTCATTCTCCTTATTTAATTTTCTTATATAATAAATTGCCAAAATAAATTTAAGCAATAAAGATAATGCCATAATAAATGCAATGCTAAAGTTTAAAAGTAAAATAATTTGAATAATATTCATTCTACTTTTAATTATTGAAAATAAATAATTTTCATTGCCATCAGCAGTTAAAATATAATTAAATCCAATAATTGCAAAGATTAAAATTGAAGAGATTATTGCTAGTTTAATTATTATTAATAATAGCAATGAAATATTCTTGTTTTGTTTAACAAACACAAATAATATTACTATTTGTGTTGTTGCCGTTACAGCAACGTTGATTAAAGAAACAATGCTATTGGTGTTAAATAATGTAGTCAATAGCAAACTTAACGCAAGCACTATTTGAACAATTGCTAAATTCAATCACGATCTAAATGAACTTTTCATTTCAAAACGCTTTTGAAATGAAACAACCATATATGCAATAAATACTACAATTGCAAAAACGCTTTGAAAAATGTAAATTCATGTTGCTTTTGAAGAACCTATTGAAATGTTTTCGTTATTATGCTTATATGCAAAAATTCTTAGAATTCAAATAAATACAGCCCCAAATAATACTAATAAAAATAGTAATTGATTTTTTAAATAGAACTTATCATATTTTTTATTTATCAATAAAGCAAAGAATTTAATATTAGATAATACTATAAAAGTAATTAAAACTAAAAATGTCAATACAATAATCACAAAATTGCTAGCAGATTTAATTGTTAAAATATCTCTAATTGTTTCATAAAATTTATTGCCTACAAACAATATGTCTTTATTAATTCCTAAATTAATTCAAGCAAATGATATTGCAACAAATAATATTGTTAAGATTCCTTGAAATGTTAAAGAAATTATTAAAATATTTTTATTTTTATAAAGTAATGGATTTGTTTTTCTTTGAATTAAAAAGTTAAATATTTCATAGCTAAAATTAATAATATATAAAGCAATCAAAATAAATGATATTGCAAATAATTTGCTTGGAAGAATTTTAAAATAGCTAAAAAATAATGTATAAGAAGTTAATGAAAGCAATAGATATAAGAAGTAAAATGGAATATATTTTTTAATAGTAACCTTATGTCAATATAAATTAGCAAAACCTTTTACTAAACTATAAAATAAGACAAAGGCTAAAATGAAGAATCTATAAATTATTAAAAAGTTTAATTGTTGATTTCTTGGACTTGAAAAGTCAAAAAATGAATTTAGTGAATTAGAAAGCAACATTTTATTAGCAAAAAGTGTCTTTTCACTAAAAATTAATAAAGTTGTAATAAATACAAGACTAATTACAATCAAAAGGTATTTCAACACCTTATTGAAAATGTTGTTTTTCTGCTGATTTTTTATTGAATATTCATCATCATAAATCAATTTTTGATTTATATTATTCGAATCGATTTGTTTTTGTTTCATCTTGTTATTTTCCTATTAAATAAATTCTCCGTCAGAATCAGAAGAAGAAGAGGTCTTTACACTATATCCCAAACCTTCTAATTGAGATTTTAAAGCTGAGGCATTATTATCAACAATTATTTCTTTTTTCAATTGTTCTGCAAACATATATAAACGCGAAAGGTTGTCAATTCCTTCTGAATTTAGTAATGAAGTTCCTGTGATTCTTACTTTTGTTGTTAAATTTCCATTACTAAATAAAATCTTGCTTTTTTGACCAGGTTCGCCTGAAACAATATGTCTATTAAAGCCTGCTTCATTTAATTCTTCACCTGATATGTTGAACGCTTCACTGTTATTATATAAAGTAAGTCTTCTTATCATTCTTGATTTTGAATTGCTTGGTTTTAATTTATCATGAAATTCTAATCCCCTTAGAGAACGTATCTTTGGTATTCTAGTAAAATCTAGGCCTTGTGGATAACTATTGCCATCTTCTTTATGATCTGGTTCTAAGCCCGGACCAAATCCACCTTCAAATATTGGTTCATTATTTCTAACTCAATAAACCATTCTCATTCCATCATTAATTCTCTTTAATTTTTCTTGTAAAGTAGAAGCATTTTCATTGTAATCAACAGGATCAAAGGCCAATGTATCAAATGTTATTCTTGTGGCGATATCAGCGCCTTGTTTATAGTCTCAAGAAACATTGTAATCATTTGAATTTATTCATTCAACTTTTCTTAATGCATTAGGGTTAATTGATCATTTATCCAATAATGAATTTCCTAAAGTATAAATTCCTAGTTCCTTTATGTGTTTATTTTCTAATGCAATTAAAGAAGAAGTGTTTGAAGAACCTGCTGAGAAAAATAGTTCTAGTAGTTGAATTTTATCAGGAATTGCCTTTAAAATATCCATAAATTTTTGGCTAGAGTCAGATTTTCCCATATTTCTAATTCTATAACCAGTAATATTGACCTTTTTAGCCTTTAAATCTTCAATTAATTTTTTAGTTTTTGCATAACCATCTGGGTTGTCAGCATCAATATCAACGATATAACCTTCATTGATCTTTCCATCTTCTTTTTTTTCTCTAGTATATTTATTGACAGTTATACCATCACCATCACTAATACCATATTGTTGAAATTCCTTAGTTACATTAGTTTTCTTTCAACCAGGATAGTCGCCATTAGCAAGACCATATGGGCTTCTATTTTCAATACTATCATATCCAAAAACTCTTCTATTTTTATTGTCATTTATTATTCTATTTGCAACACCGTTATAGCCTTCGGCTGGAGAATATGCATATGAATCTAGCTCACCTTTTTCATTTACATAGGCGTTATCTTGATCTAATGCAAGGCCTTCAGCTAATTGCTTTTCAGCATTAGAAGTAATCTTTGTAAATTTAGAGAAATCTAAATGAGAATACAATCAAAAATATTTGAAGTGTTTCCTACCTTCAAATTTAGGCTTCATTTCTGGATATTTTGCCTTGCCTTCTGCTGTTAAGAAATTAACAACATTTTCTGAATCAAATAATCTTTGGAACTTTAATAATTGGTTTCCGGTGTAACTAGACCAGCCTTTGTCAAAATCTTTTTTAATATATTCTTCGGCTTCATCTATTCAATTTGCCCCTCAACTTTTTTCATAATCTTCAAGATCTTTTTTGAAGTTTTTTTCATCTCTTTTTAAACGTTGAGTAGCCGCTTTGGCTGCTGCTTGTTTCAATTCTTCAGTAACCTTAACTTTTTTAATTTTTGGAGCACTATCATTGATATATGGGTTTAAATTAGTTAGTTGATTTAAGATATCATATTGATTATATTCACGTTTCTTTTGATCTTCAACTTCCGCTTCAACTTCAACACCGTTTAGAATAATTTTAGTTGTTCTAGTTGAAGGTTTTGATGAAGGAGGAGTAGGCTTGGGTTCGGGTTTGGGTTTTGGCTCTGGTTTGGGAGTTGGAGGCGTTGGTTGAGGTTGAGGTTTTGGCTCTGGTTTGGGTTCGGGCTCTGGTTTAGGTTTTGGAGCCGGTGGAACCGGAATTGGTTCAGGTTCTGGTTTAGGGGTTGGAATTGGTTGTGGCTTTTCTAATTCTTTTAAGTTGTTATCCTTTATTGAAGGCAACGCATTTTTATAATCTAGGTTGCTTTTATTTATAAAATCATTTCCTGTTGCTGAGCTGGTGGTATTAAAACGAGAATAATTTTGCGAACTTGTACTATAAAATATAGCCCCAGCAATAATTGAAGCTCCTATAATAACACCTGCCGTCATTAAAATTAATTTGTTTTTTCTTTTCTTTAATAAGTTTTTCATTTCACCACTTTTTTTAAATTTTATTATTGATTATTTTTTTTCTATAAATTTAAATTCATTAGGAACTTCATTATCACCTAAGCCATCAGGGAATAATGCTGTTTTAAAATCGCTATTATTTTTATATAGTTCTTTTAATGTTTGTCTAAATGAATTTTTTTGGTCTTTACCACCACCGTAAATTAGATCATATTGAGGCAAATTGTAATTTCCATACAAGCCTTCGTAGTCAAATCCTTCCGATCTAAAAGCAGCTGCTAAGCCGGTTTTTGCACCTAAATTCGATAAATAAAATATTCCAACAAGTTCGTTTTTTTGATTTCTTAAGCTTGTACCAGAAGCGCCGCCAACTGGAGCAAAATGTTTAGGCATATAATTTAGTCCCATACGCATATATGTTTTGCCATCGCTACTTTCATAGAACGAAAATACTGATTTTTGATATTTTTGGCCATCTTTCTCTTCTTGAACTTGCTTCATTCCACCTGGTTGTGCTAAAAATACATCAGAAATACCAGGTTTATCAATAAATGTTCTATATCCTAAACTATAAGATAAGTAATTTCCTCTTTCTGTTTTTGATAGAGGAAATGAAGGGGTTTCATTTTCACCAACTGCTAATTTATCATAAAATCTATAATCCGAATTTGTTCATAAACTGTAATTAAATTCATCTCTTCTTTTAATTTGGTCATCGTCTACATATCTTCTTAAGAAGAAATCTTGGGTTGAATTTGGATAACCTAATGCAAAAAGTTCATCATAATTCTTTCATCATTCTTTGTTTTCTTTATTTGCTTTTAAAGGAACATCAATTTTTTGATAATTTGATAAATATGATTTCGACAAGAATTTAATTTGTTTTTCTTTATTTTGAGCATAATTACTTGTTAAGGCTCTTGCTAATTCGTGAGCATCTAAATTGCTAAAGTTTTTAGTAATATCGCTATTTTCGCTTAGATCATTTTTAGAAACGCTTTGTAATGTTAATTTTTCAAAATCAACTTCAAAAACTGCAAAATCAGCATATTCTTCAACATCTTTATACTTTTCTTTTTGCGATTTAATTAAATAATCAGAAGGTTTTGAATTTAAGAAATCTCTTGCAGCATAAACTGTCTTTATTCCATTAGAAACATTAGTAGAATCTTTACCAACAAATGAAAATCTATATGTATTTTCATCAAGATTTGCCAAACGCAATGTTGTTCTAATTTTTGCACTATCCATCAATTTTAGAAGTGTAAATGAAGTTGTAAGTTGCTTTAATGCATCTGCAACGTGTAAATTTGTTGCAAAATATCATTTTGTTGGATATTTTCCATCACTTCTTTGTTGGTAATCCATGATTCAAGTTGTTCCAGCAGTATTAGTAAATTTTCTTTCTTCGCCTTCAATAGGAGTTAAGAATCTTATTCCAAATGTTTGTGAAGCAATGTCACGGTAATGTTCATTTGGCAATGTTCTTGCTAAACCTGTAATTTTATTTGGATCTCTATCAAGTGCATCTGTAGGTGAAGGTCCTTGCCCTCCTGGATTTTCATTAATAGCAAGGCCTTCTACTTTGCCATTTGAATCGTAGCTTGGCAATGTAAAACCTTTTCTTGCAGCTGATTCATACGAATCTTGACTAATTTCTTTTGCTTTGGCATCAAATTTTTGAATGTTAGATTGTGAAGCTTCCAAATTGGGTCTAATTTCTTTTATTGATTTTTGTTTTTGTTGACGAGTTAAAATGTCTAAATATTTTTTATTATCTAGCTCATATCTTTTTGTTTGATTTCCTTCAAGATATTGTTCTAACTCACTTTTATTCAATCTTAAAGCATCAGAACCTGTATTAGGAATTGTTCCATTTTCATCAAAACCATAAGGATTTGTTTTAAATTTTCCTACAGTTATTGTTTCAATAAATGTTTTGTTTGTCTTTTTGTCTTTAATAGTAATATTAAAATTCAATTGACCGGTTTTGTTTGCAACTGATCAATCATCAGAATTGGTTGGAGTTTGTACATATAAAACATTGACTATTTTTTTAATTTCAATTTCATCATTAAATTTTTTTTGTTTAAATTCAACACCTTGATTGAAATTTCTAACAATTTGAGATGGAAGCAAATCTTGTGAATTTTGATTTGAAAAAGCTTTGAATTTCAAATCAAATAATTGACTTGGATCATTTAACGCTAATTTTGAAACTTCAGAAATTATTTTTTTATATTCGGTAGTTTTTTGTTCTTCAGCTGATAGTTCATCTGTAGAATTATTATTTTTGTTGTCGTCGCCTTTTGAATCTTGATTATTTTGGGGTTTTTCATCAGGATTCTTTTTTTCGTCATTTTGATTATTATTAGTCTTTTTATTTGTATTTTTACATGAAATAGCTACAGCAGACATACTAGAAAGTGATGTCGCTGCTATTAATAATGATATTATTAATTTAGATTTTTTCAACCTTATACCTTCCTTGTTTTTTAATTTTAAACTTGAAAAATAGGAACTAAATTTCCTTTGATTTTTTATTGATTTCCCTATCTTTAAAAAACAAATATTTCCATTTTTCGCAATAATTTTACTATATTAAATTTAAAAAAAAAAAAAAAATACTTCAATGTTTAAAAAAATTACTTTAAACCTTTTAAAATCAATATTAAATTCTAGGGATCTAATTGAAATTAAGATAATAAATGCATATCAAGATATGCTTTAAAAAATGAAATTTTTCATTTTAATGTATAATTTAAAAATTAATATTTGAGGAGGCAACATGTTGGATGTTTGCAATTTAGCTAAAGTTTTCCCAGACAAAAAATTATTTACAAATGTTAACTTAAAATTCTTGCCTGGAAATGTATATGGAATTATTGGCGCTAATGGGGTTGGAAAATCCACTTTTTTAAAAATATTGAGTGGGGATATTGAAGCATCAGAAGGGCAAATAATTAAAGAAAAAAATGCTAGAATGAGTGTATTATCACAAAACCAAAATGAATTCGACGATTATATTGCAACTGATGTTGTAATAATGGGAAATAAAGAACTTTATCAAATCAATCTTGAAAAAACTAAAATTTATGAAGATCCAAATGCAACTATTCAAGATTATGAAAGAGCAAGCCATTTAGAAGAAAAATTTGGTGAAATGGGGGGTTGAAATGCTGAAAACGACGCCCAAACATTATTATCAAATTTAGGAATTGAACCTGAAAAATGAACTATGAAAATGTCGGATTTAAAAGCTAATGAAAAAGTAAAGGTTTTATTGGCAAAGGCTTTGTTTGGAAATCCCGATATTTTAGTAATGGATGAACCAACAAACAGATTGGATCTAAAAACAATTAAGTGATTAGAAAACTTTTTAATGGATTATCCAAACATTGTTATTGTTGTTTCTCACGATAGTGATTTCTTAGATGCAATTTGCACGCATATTATTGATATAGATTACTCTGAAGCTAAATTATTTATTGGAAATTATTCATTTTGAAAACAATCAAGCCAATTGTTAATAGAAATGCAACAAAAACAAAACCTTAAAAAAGAAGAAATTGCCCAAAAATTAAAAGATTTTATTGCCAGATTCTCTGCTAATGCCTCAAAATCAAAACAAGCCACAAGTAGAAAGAAATTACTTGATAAAATTGTCATTGATGAAATCAAACCATCATCTAGAAAATATCCTTTCATTCAATTTAACTTAAATAGAACTCCTGGAAAACAAATATTAACTGTTAAAAATTTAACTTATGTAAATGACAATGGCGACACTTTATTTGAAAATGTAAGTTTTACAATCCACCCTGGAGAAAAAATGGTTATTATTGGCAATGACGACATTGCCAAAACTAGATTACTTGAAATACTTATGGGCAAGCGTAAACCAACATCAGGAACAGTTGAATGAGGAATAACTATTAAGCCTAATTACTTCCCAAATAATAACCTAGAATATTTCAAAGAGCCAATGTCAATAATGGATTGAATTAGCAAATGACCTCTAAATAATACAACTCAAGAAACAAAAGATAATAGTGATTCAAGAATGAGAGCATTTTTAGGAAGAATGTTATTTTCAAATGATAGCGTATTTAAAAATGTTAATGTAACTAGCGGTGGTGAAAAAGTTAGATTAATGATGGCTAAACTAATGTTAGAAGAAAGTAACTTTTTAATCTTTGACCAACCTCTTGACCACCTTGATAGTGAATCTATTGATAGTTTAATTGAAGCAATTAAAAATTACAAAAGTAGTTGTATATTCACAACATACAATAGGGCAATGATAAAAGAATGCGCCTCAGTTATTTTAGAAATAAAGCCTACTTCAAGTTATTTATTCTATGGAACCTTAGAAGAATATGAAGAAGCAATGGGATATTAAATAGATAATTAAAAAACTCGCAATTAAAACTACGAGTTTTTATTTACTTTTTAAAGTTTAGCAGCTTATCTCTATAATATTCGTATTGTTTTTTACGTTGGTCAATTTCAAGAGGAAGGCCAAATTGAATGTCTTTGGTATATGTTTCAAGTTTGTCAAGGATTTCAACTATTTTATTTTGGATTTCAAGAGGAGGAAGTGGAATATTAAACTGAGAATATTTGCTAATTCACATCCTTGTATGATCATCTGATGAAAAATTTAAAAATTTCATATAATGAAAAATATATTTAAAATTATTATTTGAAGTCTTGGGAATTAAAATTTTTAAAGCAGAAGATTTAATCTTAAATTTGAAATTTACTCAATGAAAATTGCAAGTGAAATCGTCAAATATAATGATTGGATTGCTCTCAGAAGCATCAAAAATATTATTTGTTTCATTTGTATAGCCTAATATGAAACTTTTCCCTGGTGTTAGAACAGGAGTGGTGTATTTTTTACTATATTGTGTATTTTCAACAATTCATTTAGTAGGTTGTAAATAGTCAACTACTTCTTCTAATTTTTTATATTCAACTATACTATTATTGCATTGTTGATTATTCATTATCTTGTTTAATAATTCTTTATTATTATCAAAGTCTAGTAATTTATTTCTATAGTAATTATATTGTTTATTTCTATAGGTCAACTCGGTGGTCAACTCGGTGGTCAACTCGGTGAACTTGTCTAAAATATTAACAATTTGATTTTGGATTTCAAGAGGGGGAAGTGGAATTTGAATATTTTTTAATTTTTTTAAGTCAATAGCCGGTATGCCTGCCCCATATTGCAATTCCATAAATCTTTTTTCAAGATTTTTTAAATTAAAAAACAAGAACTTATTGTTTAACCCTTCAGATTTTGGAATAAACGCATAGCAATGGGCTCCTGCTCAGAATTTGCTTTTTTGTATATCTACATATCCCGCCGATGCTCCGCCCTGAGACATAGTTATTGTATCTTCATTAAAATTGTATTCTCTTCAATAACCACTCGGTGCCTGGCCTCCATTAATAACAGGATATCCCCCATCAATAGATTTAAAATTTTCTTTGTTTAATTGTTGACCTTTTTTTATTTCACATAAGCCATCTAGGCTCTTATATTCAACTCCATTAGGACATAGTTCCTTAATTAACTTTTCTATTTTGTTCATAACTATTCCTCTAATTCGGCAACTATTTTGTCAATTTCACTTCTCAATGTTTCAATCTTGGAAACTGTTTCTTTTATTTGAGAGTTTAAGACATTGATATCTATTTTTTCTGAAGTGTCTTGTTTTTCAACGTAACTATTAACTGATAAATTATATGAATTGGACTTAATGTCTTCAACACTGGCTAATTTTGAAATATAAGAGATGTCTTTTCTTTGGCTGTAATAATCAATAATATTGTTAATATTTTGACTAGTTAGCTTGTTGTTGTTTGTTACTTTTAAAAATTCTTGAGAGGCGTCAATAAATAAGATATTGTTGTCAATTTTTGATTTTTTTAATACCATTATGCATGTTGAAATACTTGTTCCAAAAAACAAGTTGCTTGGGAGTTGAATTATTGCATCTATGAAGTTATTTTCAACTAAATATTGTCTAATTTTTTGTTCAGCGCCCCCACGATACATAATGCCTGGAAAGCAAACTATTGCAGCTACTCCATCAGTTGAAAGTCAAGAAAGACTATGAAGTATAAAAGCAAAATCAGCTTTGCTTTTTGGAGCTAATATTCCAGCAGGCGAAAATCTTTGGTCATTTATTAATAAAGGGTTATCTTCGCCTTCTCATTTAATTGAATAAGGAGGATTTGAAACAATAACATCAAATGGTTCTTTGCGTTGATGTTCAGGGCTTGGAGATAGCAATGTGTCGCCGTTGTAAATATTGAATTTATCAAATCCTATATCATGCAAAAACATATTTATTCTACATAAGTTATATGTTGTTAAATTGACTTCTTGGCCATAAAATCCATCCTTGATGTTTTCTTTGCCAAGAACCTTGATTGTTTGTAAAAGTAAAGAACCCGATCCACATGCTGGGTCATACACTCTATTAACTTTTTTCTTATTGAAAACTGCTATTTTAGTAAGTAATTCTGAAACTTCTTGAGGAGTAAAATATTCACCCCCTGATTTTCCAGCATTTGATGCATACATTGACATCAAATATTCATAAGCATCTCCAAAGGCATCTATTGAATTATCTTGGTAATTGCCTAATTTCATGGCTGCTATTTCTTGCAATATTGCTGCTAATTTTTTATTTCTTTCATCGACACTACGACCTAGTTTTTGAGAATTAACATCAACGTCATTAAATAAACCTGAAAAATCATTTTCACTTTGTTGTCCTTTTGCTGATGATTCAATATTTTTAAAAATATTGTCCAATGTTTCATTTAAACAATTATTTGTTGTAGCATTTCTAACAACATTAATAAACAATTCAGAAGGCAAAATAAAAAATCCTTTTTCATTGATTAAATCATCTCTTCCAGTCAATGCTTCTTCATCTGATAAAGTTGTATAATCAAAGTCTTCAATTCCTGCTTGACGTTGGCGATTGTTTGCATATGTTGCAATATTTTCGGAAATATATCTATAAAACATAATTCCTAAAACATATTGTTTAAAATCTCAACCATCAATTGAACCGCGCAATCTATTTGCTATATCTCAAATCTTTTTATGTAATTCGTCACGTTCTTGATTATTAATAGCCATAATTTTCCCCCGGTGTAATAAATAACTTTTATATATAAATTTTACAAAAATAACCTCAAAAAAGGTTCATTATCACTTTAAACTTAAAAAACCTTTTGAATCTTAAATATACGAATGACAATGTTTCTACAAATTAAAATACAAAAAAACTCGCAATAAATGCAAGTTTTTTTTAATATATCAAACATTTTTTTAATCAATAGGTTTTATTGCTTGAAGAACTAATTTAGCAAGTTGATTGCCACCCAAAACTTCAATAACAGCTAATGCAAAATCAAAAACAGCAGCCGCACATTTTCCTGTTATTAATTTTTTATTTGGGCTTATTGATACTAATTCTTTACTACGATTAGATATTTCTTTTACACTGTCGTTTATTGGAAATGAACTAAATTTAGTTTTACTATCAATAAACTTATTTTCATATAAAACATTGGGAGCATCGCATATTGCTATAATTAATTTGTTTTGACTTTTGAATTTAGCTATTAAGTTTAAAAATGTTTGGTTCTTTCTAATACTTTTAGCAGCTGGTCCTCCAGGAATAAATAAAACATCATATTCATCAACATTTATATTTGAAGTTGTTTGCAATTTTACAATTTTATGTGCACCAGTCGCTTCAATTCCTAGAGGATTGTAAAATGTGGTCTTATTAAATAAATTATTTGCTCTATGAAATAAATCTAATGTTCCTGTTAATTCAGTATCTTCAAATCCTTCTTCAACAATTACTAATAAATTCATAATTTTCACCTCTTTTTTGATTATATACTTTTAAACAAAAAAGAATAGATAGTTTTAACTAAAAATCTTTTATTTTGTTAAATAAATTATTTTAAATTTAAAAAATTCAAGTGGAATATTTTTAACACTTGAATTTTTATATTTTTTAACATTTACTTTTTTTCTTTGAATATATATTTATATTCAAAAATATAGTCATCTTCAAAACCTTCAATCTTTTGTTGATGTTCTTTAGGTTTTGAATATTTTAAGCTAAAATCCATTTCAATTGTTTTAGGATTTGAATTCATATGAATTGTTAATTTATGAATATTAATATATAAACCAGGCATTTCTTTTCAATCTTTTGCAGCACCTAATTCAAAGTTTTTAATTGTGATATCGCCACTTTTTAAATTTGCATAAATTTTAGTTTTAGAGTTTCAATATAATGAATTATATTTAAAGAATGCTTCTTTTAAATCAACTTTTTCATTATCAATAAATTTTGCTTCGAGTAAAACTTTTTTAGGAATCAAGAAAAAAATATATTTTTTTGAATTGGATTCTTTATCTTCTAGAAGCAAATCATCTAAAATTCAATAGTCTTTTCAATATTCAAATCATTTTGACTTTGACCCTATAGCAATTTCTAAAAAATCATTACGTCAAGGACCTGCTATACTTTTATTGCTTTGCAATACTGAATTATAATGTTTGGCACTGAATAAATTTTCTATACAAAAATGCAGATTACTATTTCCAATAATATTCAAAGCATTTTTATGTTGTTTTTGTTCATTATAGCAAGATGTAACACATAATGGCAAAATTGGTAATGTTGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATCATTTATTTAAGACTCCATTTTAAAATATTTATTTTTATATTTTTTATAAAAAACATAATGATCTTTATTTAGTATTCTCTTCAAAAATAAGTTTAAATTTTGAATATGGATTAGTTCCAAAATCATATTCGCTTTTTGGGTGTTGAATATATCACAAATGAACATCAAGTTCAAGTCTTTTTTTGTTTAGACTGCAATCTAACGTTAATTGTTTAATGTAAAAGCGCAACCTGGGTTCACTATTCTTATCATCTAATTTATGCACCGGGGCCATATATTTATAAATTCATATTTTTTTATTTTTTAAATTTGTCGAAATTTTTCTAGGTTGTTCATTAAACACGAAATTAAGGTTTTTACTATAAAAGTCTGCTAAATTAAAATCTTCGCTTGAAATAAATCCTGTTTCTAATAATACCTTTTTAGGAATTAAAAACAAAATGGAATATGCATAAAAATCTATCTTTCCAAAAACACAATAATCTTTTTCATATTTATCAAAATTCTTTTTTGATGTTATTACAATTTTAAAAAAATCATTACGCCATGGGCCAGAAATAGTTGCGTCGTTATTTAAAACTGATTCTTCTTCGCTATTTAAAATATCAACCATATTAAAAACTATTTCTTTATTGCCAATAATTGTTTCGGCATTTTTTAATTCAGTTTCTCTATTATTGCAAGAGGTTACAAATAAGGGCAATATTGGCAATGCAGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATTATTTAAAATCCTTTCCCGAATATTTAATTCCCTTTTTGATAAAATGTTTCCTTGTTTTATAAACCTTTCCATTTGGTTTCATGCCCAATAAAAATAATTCGCCAGAAAGACTTGAATAAACAATTACTTGAGAATATCCTCCCCATCCATAATGGCAAAGAGGTTTATTTCCATTGTCATACATTCCATAAGCAACGATTGCATGACCAATTTTTCCTTTTTTTATTGGATTAGGCATATATGTTCCAAATATTAACCAAGGATAACCATCTCTAATTCATTTTCAAGGTTTTATTCACCCTGCAGATCTTCGATAAACATAAACAGGTCAAAAGTTTGGCTAATCATCTTTTTTTGTTTACACCTTCATTATAAAAGAACAAAAAAAATAAGATATTTTTTTATTTAAAATAATGATGAAATTAGTTAAAAAAATTAATAGTCGGTTATTTTTTTATTTTTTAATATTAAATTTAGCGCAACCTAACTAGTTGATAAAAATGATCATCAATTTATTTATAAATAATTATTTAGTTGAATTTATATGCCTCTATTTTAAGTTCTACAATTTTCTTAAAGTTATATTTAATGTGACAAAAAACCTATGCAAAAGTTATAATTTTGCTTTTTGCATAGGTTTAGGCTTATTCTAATTTATTATCTATTTTTTCTAATGCTTTTATTGTTGTTTTAAAAGAAACTTTTGCAACTTCACGCAATGTCTTTTGACCATATTTCAAAATAAAATTTGCTGCCAAATCAATAATTTTATTGCTTGCACCAAGTTTGAATTCAAAATTATATTTTTTATTTTGTTGTTGCATTCAATCTATTAAAAATTTTCTTGATATTATTGAAGCTGCTGCAACAGCCAGAGATTTTTCTTCGCCTTTTGTTTCAATAATTATTACATTTTGAGGTTTTTTAAAATCTAGTCAAGAAATTGCTTGCAATTTTTTGAAATGCTTTTGCAACATTTCTAATGAAGTTGTATATTGATCAATAAAAACATCAATGTTCTGATTTAATTTTTTTGTTAATATATTTATTGAATTACTATGAATTAATGTTTTTATTTCGTTGTTATTAATACCTGCATCAATTAATTTATTATAACCACTTTGACTAAGGCAAGTTTTTTGATAATAAACTAAATTTTCAATTTGATTAAAAATTTCAATTATTCTTTTATCAGAAAGTTTTTTGCTGTCTTTAACACCAATTTGTTTTAGTTTTTCAATGTTATCTTTTGGTATATAACAGGCAACTGAAATCACGGGACTGAAATAATCTCCTACTCCCGTTTCATCAACACCAATTAATTCATCTAAGTTTTTATTCAATTTGTTCTTCTTTACTAAAAAAGTTATCTGCACCTACAGTTTTAATATTATTTTTTTGTTCGGCCTTTTGTTTTTTACCTAATTTAATGTATTTTTCTGGGTTTTTTAGGAATTGATCAATAAAGTATCCAACTGCGCCTTCTTTATTTGTTTGTTTCATAACAACTGTTGACATCTTTTTAATTAAGGGTTCAGCATTTGCAGGTGATACACAAACATTAGCAACATTATACATGCTTAAATCATTATATGAATCTCCCATTGCAACAGTATCATCAATGTCAATGTTGTAATATCTCATAATTAGTGAAATGACTTTTCCTTTATCAATACCTATTGAAGTAATATCAAATACCGGGCTTAATCCTTCGCCTTTTGATCAAGAAGAAAATTCTCCTAAATCTCCATAACGTCTTTTTAAATATGTCAATAATTCCAAAACATCTGTATCAGGTTTAACATCAAATACAATTCCGGTTGGTTTTAGTGGAATTTTTTCTAGGTTAATACATTCTCTAAATTTTGTTGCTTGGTTAAATCCAAACACTCTTTCAAGGTTAGGATCTCTATGCATTAATTGAACCCAGTCAGGACCTTCTATTGCATAATTAGTAATTTCTTTTTTTACTTTTTCATCGCCTAAAATATAAAGAACTTCATTTAAGTCCAAATATGTAATAGCAGGTATAAAGAATGGGTCAGCAGGGTTGTGGATGTGAGCACCATTGTAATTACCTACAATTGCATTAAGTCCAAGTTTTTCATACACAGGCAATGTACTTCTTCAAGGTCTTCCTGTAATTATTGAAACAATGTGTCCTTGGGCAACAGCTTTTTTAATTGCTTCTTCTGTTTTTGGATGAACTGTGCCATTGGCTGAATCTGCTAATAGTGTTCCATCTAGATCAATAGCAAATAAAAATCTTCTTTGTTTTGATTCTTTGTTGTATGCCATAATTTCCTTTCATAATATATAAATATTTTACAATATTTTTTATATCTTCTACATTTTGAACAAAATTACTAAAAAATATGTCTCAATTTTCAATGTTTTATTGAAAAAATTTACACGGAATTAAATTTATTGCAAAATAAAAAACTTCAAGTTTTTTTACTTGGAGTTTTTTAAAAATTTATTTATTTTATTTTTGTTCCAAATATACGGTCGCCAGCATCTCCCAAACCAGGCAATATATAATTGTGTTCATTCAATTTTTCATCTTTTGAAGCTAAATATATATCAACATTAGGAAATTGTTTTGTTACTTTATCAATACCTTGTTGAACGCCTACTAAACATACTAATTTAATATTGCTAAAGCCTAACTTATCCAATTTTGCTATTGCATCGCATGCAGATGAGCCGGTTGCAAGCATAGGGTCAACAATAATAATATAGCTATCATGTGGTACTTCTGGAATTTTAAAGAAATATTCAACAACTTCGTTTGTTTCTTCGTTTCTATACATTCCAATATGACCAACTCTAGCTTGGGGAACCAAATCCATAATACCATTCAACATTCCAAGCCCCGCACGTAAAATAGGAATGATTACAATTTCTCTATCAAGAGTTTCGCCTTCATATTTAACATTCATTGGTGTTGTTATTGATATTTTCTTAGTTTGATAGTCCCTCAATGTTTCATATACCATCAACGAAGCAATTTCATTCAAATTATCTCTAAAATCTTTGTAAGATGTTTCTTTTGAACGCATCGTTGTTAATTTAGCGTTAATTAAGGGATGCTGAAAAATTTTTACCATATATTTGTGTTATCGACTTTCTTTTTATTATTTTTCTAATTTAAATAATTCTTCCATAACTGGAACTGATTTAGACATACCCAAACGGTTTGCGCCTAATCTAATCATTTCTTGTGCATCTGCTAGATTCTTAATACCACCTGATGCTTTAATTAAAAGCTTGTCACCGCAAGTTTCTTTCATAGTTTGAATGTCTTCAAATGAAGCTCCTCTATATGAAAATCCGGTTGATGTTTTAATAAAATCGGCGCCAGATTGCATTACTAATTCAGTAATTTTTATTAATTCAGCTTTTGTTAACAATGCTGTTTCAACTATAACTTTTAAAGTTTTGCCATTGCATGCTTCTTTTATTGCTTTGATTTCGTTTAATACGTATTCATATTGTCCATCTTTAAATCTTCCAACATTGATTACCATATCAATTTCATCAGCACCATGTTCAACAGCAATTTTTGTTTCATAAACTTTAACACTGGTTGCATTATATCCTAGTGGAAAACCAATAACTGTGCAAACAAGCACATCACTGTTTTTTAATGCTTCTTTAGCATACTTAACATATGATGGTGCAATACATACTGATTTAAAATCATATTTAATTGCTTCTTGAATTAATTTATCAATGTCTTTGCTTGTTGCAGAAGGTGATAAATTTGTGTGGTCAATATATTTGTTTAATTCTGTCATATTCTTCCTTAAGTTAATTTATTTAATTACATTAATTTAGCAAAAACCGTTTGAATGTCATGCTTGCTTTCGCTAAATTCTATTGCAGACAACAATTCTTTTTGAAGTTCTGGATTTATAGGTTTTGACGAATACAATGTAAATAATACATCTCCTTTATTTACATATTCATTTGAACTCTTATTCAAATAAATTCCTGCTTCATAGTCGATAGAATCTTCTTTTTTAGATCTACCTGCTCCCAATTTCATTGCTACTAATCCAAAATCAATTGCAGATTTTATTTTTAAATATCCACTTTGGCTTGCAATAATTTCTTGTTTATATTGAGGATTAAATCATTTTGAATCTTTTTCAAAAACCTTGATGTTTCCGCCTTGTGCTGAAATTCATTCCAAAAATTTGTTATATGCTTTTTTATTATTGATTACTTCATCAATCATTTTTCTTGCTTCAACTTCATCTTTAGCTTTTTGTGCCAAAACTAATAATGTTGAACCTGATGAGTAAATAATTTCTGTAAAATCTTTAGGTCCGTGTCCATTTAATGTATCTATTGCTTCCAAAACTTCAATTTTATTGCCAATAGTTTTTCCTAAAGGTTGTTGCATATTGGTTATTTCAACGGCAATCTTTCTATTTAATTTCTTGCCAATTTCAATCATCAATTTTCCAAGTTTTTTTGCTTCATTAATATCTTTCATAAATGCGCCATTGCCACATTTAACATCCAATAAAATGCAGTTTGAACCAGTTGCAAGTTTTTTAGACATAATTGATGAGGCGATCAATGGAATTGATTGAACGGTGCCAGTTACATCACGTAATGCATAGATTTTTTTATCCGCAGGCACAAGTTTTTGATTTTGTCCAACTATTGCAATACCATGTTTTTCAACTAGGTCTCTAAATTGTTGGTCAGTCACTGAAAAATTATAACCGTCAATTGATTCTAATTTATCAAGTGTTCCTCCTGTATGTCCAAGTCCTCTTCCTGACATCTTTGCAACAGTTAAACCTAAGGCTGCTAAAATTGGGCATAATGCAATACTTACTTTATCTCCAACTCCTCCTGTTGAGTGTTTATCAACGATTACTTTATTTAAATAACTTCAATCAAGTACTTCGCCTGAATGCATCATAGCGTCGGTTAATTGTGCTGTTTCAGCATCGCTTAAACCATTTAATCTGATTGCCATTAATAAAGCAGCAGCTTGGTAGTCAGGAACATTTTCTGCTACATATTCTTTAATGAAAAAATCAATTTCTTCTTTGGTAAGAGTTTGATTTTCTACTTTTTTGTTAATAATATCAATAATTCTCATAATTATTTCTTTCTATTTATTCAAAATCTTCTGATATTGATAATGCTAATTCCATCATTTGATTGAATGAATTTTGTCTTTCTTCAGCGGTTGTATATTCATGTGTTATTAAATTATCAGAAATAGTTAAAATTGTTGCAGCTTTTTTGTGTAATTTTTCTGCTACGGTAAATAGTCCATATGCTTCCATTTCAACAGCAACTGCTCCGCCTGAATTTTTAACTCTATCTTCAACAGAAAATACTGAATAAAAGGCATCTTCCGATAAAATTCTTCCTTCTTCAAGTTTTAAATTCAATTCCTTAGCATGTTTTTTAATTAAATCATTTAATTCGCTATTTGGAAGCGCAACATTACTTTCATCATTGACTAATAAATTTTTACGGAATGAAGTGCTATCACTATATACTGAAGTTGCCAAAACTAATGAATAATTTTTTAAATCAGCCTTGAATGAGCCACCTGATCCAATTCTAATTATTGCTTCAACTCCATATTGACTAAATAATTCATATGAATAAATTCCAATTGAAGGAACGCCCATGCCTGAAGCACAAATAGAAATTTTTCTTCCCTTATAAAAGCCAGTATACATTAAAACATTTCTAACTGTTGACACTAATTTAGCGTTTTCTAAATAGTGTTCAGCAATATACTTTGCTCTTAAAGGATCACCTGGCATTAAAACTAATTTTGCAAAATCATCATCTTTTGCATTTATATGAGGTGTCATATTTTCTCCTTATTCTATTGTTAGTTTTTTATACATTATTATTTTATAATAATTAAATAATTAATAAACATTAATTGTCTAAAACGCTAATAAGTCAATACATAAAAAAGCAAAAAGATTAACTTCTTGCTTTTTTATGACACTATAAACCGCTTTCTGTACTATTTTAATAGCGCCAACTATTTATCTAAGCCAATCGGCTTCCGGGTTCTTTGTTCGGTTCCTTGTTCCCAGTTCCCCTACCAAAATTTGGGTTTCTAGCTCATGGAGTTTACATCGTTGCATCTTAACTAAATAAGCTCGTCTCTGTTGCACTAGTCGTCAACTAAATTGTTGCCTAGACTTATTGGGTCTAGCATGAACACTACTCGCATCACAGCGAGTGCTAGAGCGGATTTTCCTCTACTTTGTAGTAGCAGTTGGCCATGCAATTTAATTTTACCTTAAAACACAAAAAATATCAATGTTTTTTTAGTAAAAAAATATAATTTTAATATATGGAATTTGAGCTAAGTGGAATTGTAATTAATAAACAAAATATTAATGAATTTGATGAAATAATATCATTACTAACAGATAAAGGCATTCAAAGAATTTTTTGCCCCGGAGTAAATAAAACTAATTCAAAAAATGGCCCATCAATTTTAATTTTAAACTTAGTTAATTGTGAAGTCATTAAATCAGAAACAAGAAATTTATTGCCTAGACTAAAAACTGCTTCATTAATAAAAAATTTTCCCACAGATTATAGACTTTTAAATATTGCAAATGATTTAAAAACCTTCTTTAGTTGTCTTGATAATTGCAATAATATTAATCTTATTAAAGAATATGGCCATTGCTTAGATTTTATATTGTCAAAATCTGATAAAGTTGAAGCATATTTATTGTTTTTAGTTGTTTCTAGTTTAGGATTTAAGCCCAACTTAAATTCTTGTATTGAATGCAATAATGTTGAAAATATTGTAGATTTTAAACTATACAAAGGAGGATTTTTGTGTTCGATGCATTCAAACATTGTAAATGCTGATGTATCACTATTAAGGTCTTGATATTGAATAAATAAAAAATTTGAACAATTTGATTTAAATTGTTCAAATCTAAATGCTATTAAAATCAATAATGAACTTAAAATGTTTTTGTATGATAATAACATTATTTATCGTTAATAGATATTTTTTTTATCTCATCAAACTTAAGTTGTAATGTTTTAAAATCAATATTATTTGTTTTATTAGAATATAACATGTCTTCAAAATCATTTAATTTTATTAAATCTTCCTTTGATATATTCTTTTTATGTAAATTAATAATTAAATTAAATGCTTCATTATAAAATAGTAAGTATTGCTTAAAATTATCTTCTAAATATTCATATAGATTTTTAAAATCATCAGAAATTTTAGTCAATGTTTCCTTGTCTTGTTTATCTTCAATATATTGACTAAAAATATGTTCATTCTTATCCACTATTTTTTTCTTAAAATCATATTGAGCAACTTTTTTTTCAAAATAATCTCTTAAGTTTCAAAATATACCATAATTAGGCAATGAATGAATTAAATTTAATGAACGTAAATATTGCTTATAAATTTCTTTACGAATTATTATTAATTGACTGTTTGAATTAGTTTTTTTATGATTATTAACAAGAACATAAGTAATACCTAGTAAAAAAGGCAATCCTATAACTGCACTAATTCCTGAAATGATGCCTATTTTTGATCTTCTAGTTAAAAATCTATGTGGTTTTTTATTTTGAACCTTTTCTTGATTTTTTGATAACTTCATAACAAAATTATATAGTTTAAATTAGTATTGTTATTTTAAAAATAGTAGAAAAATTTATTATTTTTTAGTAGATTTTGCCAATAGGATAACATTTTCATCAAGGTTTGGATCTTCTTCAATACTTGCAGTAGTATGATTTGTAAACAAAATTGTAGGAATAATAACAACGTCTCTACGTTTATTTTTATAAAAGAATGGGGCTAATCTATCAATTATTAGTTGCTTTAATTGAGGCACAGTTCAATCATTTGAGTTTTTAATTGTATATAATATAGCGCCATGAACCAATCTCTTTGCTTCTTCAATTAAATGCAATGAATTTTTTACATAAAATGCACCACGAGAAACTATTCTTGGTTTACCAATAATTAGATTCTTTTCTTTATTAATTGCTAAAACAACGTGCATAAATCCATTTTGGCCTAATTCACTACGTTCTTTAATAATTTGAGAGTTGGTTTTGGATAAAATATTACCATCAATAAAAACAGGCCCAAAATAAACCTTTTCATTCGACAAACGAACTTGATGATTTTCTAAATAATAAACATCCCCTAATCTAGCAATCAAAATGTTTTTAGCATCAACACCGCTTTCAATAGCAGTATAGCCGTGAACAACGCTCATTCTATATTCGCCGTGATAAGGCATAAAATATTTTGGTCTAGTCAATTCAAAAATTTTTCTATGTTCATCTTTGTAGGCGTGGCCCGAGGTATGTAAATAACCGTCTACTCCATTTTCACGAATTATTGCGCCTAATTTATATAGTCTATTAACTAATAATTCAATTTTAGATCTATTTCCAGGAATTGGACTAGATGAAAAAATAATAGTATCCTTTGGTTTTATTGTAATATTTGGATGTTTTCCTCATGACATCTTTGCAAGTCCAGCTAGTTCTTCACCTTGAGAACCAGTTGTTAAAATTAATAATTCATTATCTCCAAATTTGCTTAATGATTTTTTGTCAATAAACACTTCGTCTGGAACATTAATATAGCCTAGTCTTCTACCAATATCTATTCCATCAACCATAGAACGGCCAAAGGCAACAACTTTTTTATTTAAAGAAGCGCCTAATTCAATAATTGCTTTAATTCTAGTTAAATTAGAAGCAAATGCAGTTACAATGATTTTCTTTTCTGCTTCCAACATATATTTTTTAATATCTTTTAAAATATCTGACTCTGATGGTGAATGATTTGGACGCATAGCATTTGTTGAATCAGAAAATAAAACATCAAGACCTTCATCGCCAATTTGTTTAAGTTTATTAAAATCTGTAAAATTTCCAATAGGTGTATAGTCAAACCTAAAATCACCTGTCATCATTAATGAACCATTAGGGGTTTTAATTCTAATGCCAAAGGCATCGGGAATTGAATGTTGGGCAGTTCAAAAATCTACTTGACATGGGCCAAAATTTCAAACTGCATCTTTTTCAGTTTCAATAAATTCAATATTACGTTTTATCTTCATATCCTCAAATTTTAGTTTTAGATATTGAATAGCAATTCTAGGTGCAAATATTTTTTTAACATCAACTTGTTGAACTAAATATGGAACACCTCCAATATGATCTTCATGTCCGTGGGTTATGAAAAGACCTTCGATTTTATCTTGATTTTCTGCTAAAAAAGTGAAATCTGGAATAGAACCATTTACACCTGTTACATATGAATCGCAAAATTTAATTCCGGTGTCAATAATAAAAATATGATTTTCATATTGAATAACCAAAGTACTTTTACCAATTTCTTGCATTCCGCCTAATCCAAAAATTTTAGTTGGGCTCATAAATCCTCCAATTTTTATAATTATTATAATGAATAAATATTCAGCTTTTTATTGTTAATATTTTAGCAAAAAAATACGAAATTAAAAAAAATACAAATTTTTTATTAAAATTTTAGATATGAATGATAGTAGGGTACAAAAAACAAAGATTCCTAAAATGTTAGCAAGTTTTAGTTTAATTTTAGAACTTATTGCATTAGCATTACTTATTTATGTTATATATAGTTTCTCTTCAATTTGAAAAGAAACACTTGCAGATATAAAGGGAATTATAAAAAATAATAAAGACAGTTTTGAAGCATTTTGAAAGGAATTTTCTAAATATTGAATAGACTTTTATAAAAATAATAAGGTCTTAATTAATGCAATTTTAGCATTGCCAATATTATTATATGTTTGCTTTATATTTAGTATGTTAGTAATAGCAAAAGCTAATTGGTCATTACACAAAGTTCCAATAATACTTATTATTGTTACATTATGTTTGCCAATTCTATTATTATGTGAAATAGTTAGAATAGTATCACTATTTTTAATAGTAATAAAAAAGCAAAAAAATTTTAATAGCCAAGTATATTATGGACCACAAGTTTCTAACATACAAAATTAACAAAAAATTCGTAATTATTTCTATTACGAATTTTTATTTATAAAATAAGAAAACAAAAAGAAATCTTATTTATATAATTTATCGATTTTATTACTTATTGCTCTTGCAGCAATTGTCCCGTCGGCTGTTGCTGTTGTAATTTGTCTTACATCTTTTACAATAACATCGCCTATTGCAAAAATATTAGGAATTTTAGTTTCCATATTTTCATTAACTTCAATAAATCCACGGCTATTTAATATTTCATCGTATTTACCTTTTATAAATGCTGTGTTAGGTTTAAAACCAACATAAGGAAAAATGCAACTTATATTCATTTTTGTTTCTTTTCCATTTACATTAGCAATAATTTCTTCAACTTTTTCATTGCCCAAAATTTCTTTAATAGTTGAATTTTCATGAATATAAATATTACTTTTAGACATTGCTTCATTGACAAGTTTTTTTTCTGCAATAATTCCATCACGAACAAAAATATGAACCTCAGAGGCAATTGATGCTAAATAAGATGATTCTTCGAATGCTGAATTACCACCGCCAATGATTGCACAGGGTTTATTTTTATATAAAGGACCATCGCAAATTACGCAATATGATACACCTTTGCCTCTATATTCTTCAATACCTTTTACATCCAAAGGAACAATTTCTTTCATGCCAGTTGCAATAATTATTGCTTTTGATTTTATTGACATGCCATTTTCCAAAATGACTTCTTTTTCAATATCTGATATAGAATTAATTTCCTTTACATTACCATAAATAAATTTTGAGCCATTATCTCTTGCTTGTTTAAGCATGGCTTTAGAAAGTTCAAAACCACTTATAAAACTAAAACCTGGATAATTTTCAATTTTAGATTGAGCAGAAACTTTTCCTCCTGGTACTCCGCCTTCAATAAAAGCAACATTTATGTCATTTCTTGCTAAATATATTGCAGCCGTTAATCCTGCTGGCCCTGCCCCAATTATTAAAACATCATATAAGTTATTATTTTCCATATATTTCTCCATTACTAAAAAAGCAGCAAATTATTAAGCTGCTGTTTTAGTTTCTATTTGATTTTTAGTAGTCGATTTTTTAAATGCAAATGAGTTTGAATTTTTAGGTTCATAAACTATAAAGTAAAAGAATTTTGAAAGCTTTTCGTTATTATATGTATTAACTTTATAAACATTGTAATTCAATTTTATAACGAATTCAAACATTACAGTCAATACCAAACCAACTATTATATATAAAGCAGAAATTACTTTATAAATAGTATAACTGCCATCACGTAAAGGTTCCATTCCAAAACGAATTATTCCATAATAAACAATATATAAACCACCAGTAGTTCCTGGACGTAATCAATTTCAATTATTCAATATTCAAACTAAGATAATATAAGCAGCTAAGTTTGCAATTGATTCATAGAAGAATAAAGGATAACGGTATTTTCCATCAATAAACATATGATCTCTAATTAATTTTGGCAATATTTCAAATGCGACAGAACCTTTTTCCATAATGCCACCATAAACTTCATGGTTTGCGAAGTTTCCTCAACGTCCAATTGCTTGTCCAATTAATACCGCTGGTAAAATTATAGTAATTGCTTTTTTTAAATCAATTCTATTTTTATTTTTAGAAAATAGAATATAAAGAATACAACAAATTGTAGCAGTTATGACACCACCATGTATTGATAGTCCACCCTTTCAAATAAAGAATGCATTATACCAAGGACCATCTGCCCAACCTTTATGTTCAATTAGTTGTTGAATAACGAATACAAATCTAGCTCCGATAATAGCAGTAGGAAGTGCTATAAATACTAAAATTGTTAGTTGTTCGAAAGTATATTTTTCTCTTCTTCAGAAAAAATAAACAGTCAATATAGCAGTGAGCATTCCTAACATCATTGTTAAGGAATAAACATTCATTGGGCCTAATTTTAATTGAATTCCTGCTCAATCTTTAACTTGCATTTTCCCTCCTTATAAATTTGCCACTTACATTAAAATATAATTATAAATTATACAAATCAAAAAAATTTTTTTTAATAATCACTATAAAAGTTTTGCTAAATATTGCCCCGTGTACGAATTTTCGCATTTTGAGACCTGTTCAGGTGTTCCTGAGCAAATAACTTTTCCCCCTTCGTCCCCACCATCAGGGCCTAAATCAATAATATGATCAGCACATTTAATTAAATCCAAATTATGTTCAATTACAATTACTGTATCGCCATTGTCCACTATTCTATTTAATACTATTAATAAATTAGAGATATCGTAAGGATGTAATCCGGTTGTTGGTTCATCTAATACATATAAAGATTTGCCTGTTGGCTTTTTTTGTAGGTATGTTGCTAATTTTACCCTTTGAGCTTCACCACCACTTAATGTCGTAGCATTTTGGCCTAATTTAATATAGCCAAGTCCAACATCTAATAAAACTTGCAATTTTTCTTTAATTTGGCTCTTGTTTATAAATAATGCATAGGCTTTTTCAATTGTCATATCCAAAACATCGCCAATACTTTTACCGTGATATTTAATTTCTAGTGTTTCTTGATTATAACGTTTTCCATTGCAATGGTCACATGTCACATAAACATCAGGCAAAAAATGCATGGCAATTTTGATTACACCATCGCCTTCGCATTTATCACAACGTCCTCCTGAAACATTAAATGAAAATCTTGATTTTGTATATCCTCTAATTTTACTTTCTTCGGTATTTGCAAAAATTTCTCTAATATCATCAAAAACACCAGTATATGTTGCGGGATTTGATCTAGGAGTACGTCCGATTGGATTTTGGTCAATTTGAATTAATTTATCAATGTTAGTTTGGCCAATTAATTTACTATCTCTTTGAATATCATAATATTGATCTAATAAATATTTATTAAGACTTCCAACTAGTTCATCGTTTATTAATGTAGATTTTCCAGAACCACTAACTCCAGTAATTGCAATAAATTTTCCTAATGGAAAAGTTACTTTAATATTTTTTAAATTATTTAATTTTGCATTTTTTAATGTTAATGTCTTGCCATTTCCACTACGTCTAAATTTTGGCACCTCTATTTGTCTTTCTTTCGACAAATATTGGCCAGTTAGGCTTTGCTTACATGCAATTATATTTTCAACATTTCCACATGCAACAATATTTCCGCCATGTTCGCCTGCTTGAACACCAATATCAACTACATAATCTGCTTGTCTAATTGTTTCTTCATCATGTTCAACAACAATTAAACTATTGCCTAATTCAACCATTTTTCTTAAAGAGCTTAGCAATTTTTCATTATCTTTTTGATGTAAGCCAATTGATGGTTCATCTAAAACATACAAAATACCTGTTAAATTTGAACCAATTTGTGTTGCTAATCTAATTCTTTGAGCTTCACCACCGCTTAATGTCTCAGCTTTTCTATTCAATGTTAAATACTTTAAGCCAACATCAATTAAAAATTTAATTCTATGTTTTAATTCAGACAAAATTAAAGAGGTAATTTCTTTTTCTAAATCACTTAACTGCAATCTTTCTAAAATAATTGCTAAATCTCCAAGCGACTTATTGCAAATTTCGTATATATCTAGATCTAGAATTTTGCAAGACAATGCATACTTATTCAAACGCTTTCCATGGCATGAGGGACAAGTAATATCTCCAATGTATTTACTATAATAAGCCCTAGCTTCTAGTGATTTTGTATCAAAATACTTCCTTTGTAATTTATCAACAATGCCTTCAATAACTTGAAACTTCTCGCGTTTTGTGCCACTTTCGGATAATAACACATATGATATTTCTTCTTGCGACCCATGTTGAATAATATCTAGTTCAAAAGAAGATAAAGAATCAATAGGTTGATCTTTATCAATATGATAATAATTTAATAGCTTGTCAAATTCTTGTCATTCTAAATTGTATGTATTCATGAAATTTCTATAATACTTTATTGCCCCTTCGTTTATAGAAAGCTGCCTATCGGGACAGATTAAATCAAAGCTTGCCTTTTGAATAACACCAAGCCCCTTGCATTCAGGGCACATTCCAGCTGGAGAATTAAACGAGAATAATTTAGGTTCAATTTTTGGCATATCAAAGTCGCCAAAACTGCAACTATGATGTATTGAATAAGTATTCTTTTTACTTTCATTAACAATAGTTGAAATTAAACCATTAGATTGCTCTAATGCAACCTCAATTGCACTTGATATTCTTGATTTTATATCATCATCATTTGATAATTTAACTCTATCAATAAACAAATCAATATTTCAACGTTTATTTTTATCTAGATTAATTGAATCAACATTTTCTAAGAAATAAATCTCGTCATTTATCAAGACTTTTATAAAACCATCTCTTTTTAGTTTATTAAGTAATACTTGGTGGCTTCCTTTTTGATTATTTACTAATGGGGCTAATATGTATATTGTTGATCCTAATGGGTTTTTATAAATGTTAGAAATAATGTCTTTTGTTTTTTGAGCAGTAATTTCAATTTTGTGATTTGGACAATAAGGTTTTGCTACTCTTGCATATAGCAATCTTAAATAATCATAAATTTCAGTAACAGTACCAACTATACTTCTGGGATTAACATGTACTGTTTTTTGTTCAATAGAAATTGCTGGGCTCAATCCTTCAATGCTATCAACATTAGGTTTTTTAGTACCACCTAAAAATTGTTTTGCATAATTAGAAAGGCTATCAACATACCTACGTCTGCCTTCTTCATAGATTGTATTAAATGCTAAAGAACTTTTACCCGAGCCACTAACCCCAGTAAAAACCACAAATTTGTTACGAGGAATATCTAAATTAATATTTTTTAAATTATTTTCTCTAGCACCTTTTATTATTATTTTATCGTTCATCTTCTTTGATATAACCCTTTTCTATTAAATATTGTTCTGCTTTCTTTATTCTTTTTTTAAAAAATTTATTAATGAATTTTAAGTATTTCATATCCATTTTAATCTTATTAGGTTGCTTAAATTTAAATTTTTTAAGTTGTTTGATTCAATCTAAATGTCTTTTTCTAATAAAAATTTTTAGTTGTTGATTAAAACTTTCAAAAAACCTAGTCTTGCGATTCTCATAGTCATTTAAAAATGCAGTTTTTGCAATTGATTTTAAATAAATAGGATAATCAAATATTAAACTATTGTTGAAATCAAATATTGGAGCTGGCTTTGTTAAAATTCCGCTATTATTGTCCAACATCATGCCAAAATTATTTAAATGTCTATCCCCATTGCAAATTAAAGCATCAAAAACCATTAAATCTTCAAATAACTCTTTGCCATAATAAAAAGCAATCTGACTACATAGTTTTAAATTATTATCAATACTATTGGGATCTATGATTTTAGAAAATGGGATAAATGAAAAATCTTTGTTTGTAAACAATTTGCAATCACTAACAAAATGCTTTCCATACTTAGTAATTTTATATTCAACTGCCGGTAAATTCAAAAATTTGGCAACTTGACTTGCAAAATGTTCACTATAAACATCGTATAAGTTTTTATTTGCAAATTTTGAGTTTCGTTTATATAAATGAATTTCGCCATCCACCTCAACTCATGCTTTTAACATGTCGCCATCAATAAATCAACTAGGGCTTTTAATTTGTAGTCTGTTAGTTTCTGTTAATTTTGCTTTATAATCTAAAAAATAATTGCAAGAATCATTATTGAAATGATCAAAATAGTTTACATCTTCTCAATTTAATTTGCAACTTTCTTCTTGGCTGCATATTCAAAATGAATTGCACAGATTCAGATAATTATTATTTAAAATTAATTTCTTATAATTTAAAAAGTTTTCAATTAATCCCTTGTAATTAGGCCTTTGCATTGGAATTAATGAAATATTTAAAATGAATTCAAGTCATTGTCTTGGCGAATATTCATTGTATTTTTGCTTTATAAAAATAAAATCATCAAGCAATTTAACATTGCTCAATGTTGTTAATTTTTCATCTGCTTCAAAACTTAAACAAATCTTGTTTTTACATTTTAAATAATATGTTTTCATTACTAACTAATTTATTTCGCCATCAAAATCGTTCTTATTATCTTTTGATTTTGTTTTTGGATCTTCTTTGTGGTCTTCCTTTGCTTGATTTTTATACTTTTCTTCAACTTCTGCAATTTCTTTTGAAAGATCACGGTGCAATATTTTTTGTAAATTTTCTAGCATGCTTTTTTCTCATGAACCATCATTAGGAATATTATTTATTCCCATTCCCCTTAGTTCATCGAATGCGCCCTTGAAATAATCAACCATTTCGTCATCACGAACATTTCCAATTGCTTTGGCAGCTTTATACATACTATCAACCAATTTTTCCATACTTAGATCTTCATAATTCATTTCAAATGGAGAACCAGACATAAATTTATTATCAGCAATTTGTTCTTTTAATTTTTTTGCTTGTTGCTTTAAATCATCAAAACCTTCTAAGAAATTTACAAATGAATTTTTAAAATCATCCATACTGCTCATATCTATTTCTTCATCATTTGAATCGCCATCATCTGATTTTTCATCAGATTCTTCGTCTTCTGATAATTCAGATTCAAGAGAAAATTCCTTAGTTCCAAACCCGCCATCATAATCTTCGATTGACATTCTACGTTCAATAGGGAATGCTTCTTCTATTGCTCAAATTTTGTTTCCTAATAAATTTTCTACTGAGTTTCATGCATCAATACCATTTTCTATTTTTGTTAATTTAAAATCAAAACTAGATGGTTCGGTTTGCAATAAATTAAGTTTTTCTGATAATTCAAATAATTCGTCTCATTTGCTAAAATCACTTCTAGTTATATTTTTAATATCATTTGAAGGGTTTGTGTGAAAATTTTTAAATTCATTTTGCTTTAATTCTTTTAAATATTTTAAAGTTTCTTTTCTTTCGGAAATTACACTTTGACTAATTAATTCAGGATCAATTTGAAAATCTTCATCAAATTCTCTTTTATCGTCATAAACAAAATCAATTTTTAATGCCAATGAAATAATTTCTTTTGTAGGCAAAATTGCAGAGAAAATAATTTCATTTTGTTCAAGTCCATATTTTTTAAAGTCAAAAGAATTTTTATTTTCTTCATCATCGTTTATTAAAAAATCATTAAAAATATTTAGGGAATTAAAAGTGTTTAAATCTGACAAAAATTCTTGTGATGCTTGTAAATCGCCAAATACAAATTCAAACGGAACAATTGTTTTTAAATAATTTGGAATTGTATATCAATGAAATACAACACTCTTTTCAAAGCCAAAAAATGCGCCTAAAACATCCACTAATTCAGAAAAAGAAACCCCTGAAGTTATTGGAAATGATATTGGTTCAAAATTAATTTTTTTAACTGCTTCTATTGGTTTAAATTCGTAGTCAAAAGCTACATTTAATTCTCTAACTGTTAATTTTAGATTTATTCCTAATTGTTTCTTAATCATTTTCTGCTCCTCTATTGTTTTCAAATTCATGCAACATATATTTTTTGGTTATATTTTTTAATCTATATATGTAATCAAGTATTGCTTCTCCCAAACCATTTTGTTCAACAGCACTTGTATGAAAGTGGCTATGTTCATGTACAGTTTGTGTTGCATTTGCCATTGCATATGAATATTTTGATATTGACAACATTGGTATATCATTATTTCCATCCCCGATTGTCATTATTTCATCTTTAGATGTTTCTACATTCTTATAAACATTTTCTAACATTCAAAGAACAGCAGACCCTTTTGAAATATTTAATGGTGATATTTCAATATTGCAATTTGTTGGAATCATTGCCAAATTTGTTATTGACTTAAATTTTTCAAACATTTGCAATGCTGATTCTTCCGTTTCAGTTCCATCTGCTGAATAAATTTCAATCTTGGTTGGTTCAATAAATTCACCTTTATATAATTTAGGAATCGAGTGTAATAATTCCTTGCTTATAAAGTGATATGAGCAAATATATTCAATTGATTTTTGATTATCTTTTGTGTAATAGTAATTATTATTGTCTCATAATAGAACTTGAAAATCATTCTTTTTAGCAATGTCAAGAATCAATTTTGTTGTATCAAAATCTATTTTTCAACAGGCAATATATTCTCTTGATTGCATATCAAAAATTTGAGCGCCGGAAGATCCTATTAAATATCTCGCTTTTAATGTTTCGGCTAGTTTCAACATTCTTTCTTGAATTGGATTTCCGGTGCATATATTAAAATCTAATCCATTGTCTACTGCAAAATGGATATCATCAATAGTTTCTTGCTTTAAATTAAAATTTTTATATATAGTACCATCAACATCTGAAAAAGCAATTGGCTTCTTGTTGTTAGTAACTTTTAAAATTGTTTGACTCATAATTCTCCTTGTTTTTTTATGCTCTTGTCTTTGATTTATGCCTCTTTTTTAATACCATTATAATAATATAAATTATAATGCTAGAAATGGCAACTGGTGGCACAATATAACTTAATAATCTAAATGTATAATGCATTGTAGTTTCGGGTTCTTCTGATTCTAAAAATTCCGTATAATCTAATAAAAAATGATTTATGGCATATGTTTTTAAATTGAAATAATTCTTTACATTTAAATCCGATGCGTCCTTTTTTATTTCATTACTTAATTGTTGTTGCATTTTTTTTGATACTAATTCAAACACAAAAAGTATTTTTAAAAGTTCATCATTTGTTCTTAATCTTATTTGAAAATATTTATTAAAAACCCTATAAAGATTAGGATCAGGATCATTTTTTAATAAATTAATAAAACTATTTTGATTCTTTATATCAAAGAAGGGATATTTAAGATCATTATCCAACTCCAAAGCTCTATTGTATTCAGACTTTTCTTTAAAATTCCAATCTTTTACTCTATTGTTATAAAAATCTTCAAATCTTATTTTTTTAAATGGTTTTTTAGGGTCTTCTATATTTTGATTTTGTAAGAATAATTTTTGATTCATTTCACCAAATGAAAATCTGTGTTCTTTAAATATTTCAACTATTCTTTTAGCAACACTTAAATCTTGTTTTTCAATTCCATCATCATTGCCTAATTCTAAATTATCTTGATTATCTTTTGATTTTTTGCATGATAATGAAACTAATGGGATAGCTAGTAAAGTTGGAATAGTCAATGATAAACCCAAGAATTTTTTTATTTGTTTTTTATTCATAATATTTATTTATTATATTTTACTAAAATTCCAAACTTAAAAGATTTAAAAGGGAAATAAATGCTAAAAACTATACACCAGAAGTTTTTAAAAAATAACAAATTAAATCAAATTTCAAACTTTATAATTCCTATTCCATTATTATAGATACTTAAATCCCAAAAGTTTTCAACAAAAATTAACCTTTTAGTTCAAAAATATAGTTTTTTTTATTTTTTTCAATATATCGAATATAAACCATTTAAAAAAACAATAAAATATTCCCGGAGATTATTAAGTCTCTTTTTTATATTTACTAAATTTAAAAAAGGATAAAAAATTAAAATGAAAAAATCAAAAAAAATATTTATAACATTATGTGGAATAGCTGCTACCGCTGTTTTGCCAGTTGCTACTATTTCATGTAATGATGATAAACTAGCAGAGAAAAATGGAAAAGAAAAAGCAGATGCTGCCTTAAAACAAGCAAATGCATTAGCAGAAGAATTGAAAAAAAACCCAGATTATTCAAAAATTTTAGAAACATTAAACAAAGAAATTGCTGAAGCAACCAAGTCATTTAAAGAAGCAGGTTCATACAGCGATTATCCAGCAATAATTTCTAAACTTTCAGCAGCAGTAGAAAATGCAAAAAGTGAACAACAAAAAGTTGATCAAGCAAATAAAAAAATTGCTGATGAAAATTTAAAAATTAAAGAAGGCGCTAAAGAATTATTGAAATTAAGCGAAAAAATTCAAAGTTTTGCTGATACAATTGCATTAACAATTACTAAATTAGAAGGAAAAAAATTCCAAATTGATGAAACATTTAAAAAACAATTAATTTCAACAATTGAATTATTAAATAAAAAATCAGCCGAAGTTAAAACTTTTGCAACAGTAAATACCATTAAAAAAGATTTCCTTTTATCAGAACTAGAATCATTTAAAGAATTTAACACCTCATGACTTGAAAAAATCGTGTCAGAATGAGAAGAAGTTAAAAAAGCTTGATCAAAAGAATTAGCTGAAATTAAAGCTGAAGATGATAAAAAATTAGCAGAAGAAAATCAAAAAATTAAAGAAGGTGCTAAAGAATTATTAAAATTAAGCGAAAAAATTCAAAGTTTTGCTGATACAATTGCATTAACAATTACTAAATTAGAAAGAAAATTCCAAATTGATGAAAAATTTAAAAAACAATTAATTTCAACAATTGAATTATTAAATAAAAAATCAGCCGAAGTTAAAACTTTTGCAACAGTAAATACCATTAAAAAAGATTTCCTTTTATCAAAACTAGAATCATTTAAAGAATTTAACACCTCATGACTTGAAAAAATCGTGTCAGAATGAGAAGAAGTTAAAAAAGCTTGATCAAAAGAATTAGCTGAAATTAAAGCTGAAGATGATAAAAAATTAGCAGAAGAAAATCAAAAAATTAAAAATGGTGTTGAAGAACTTAAAAAAATCAATAATGAAGCTTTTGAACTTTCAAAAACCGTAAACAAAACAATTGCTGAAGTAGAAAAGAAATTTAAAATAGAAGCATCATTCAAAGAACAACTAGAAAATTTTGCAGATGACTTATTAGACAAAAGCCGTCAAATTGATGAATTCACCACAGTTACTTCAACTCAAGAAGGTTTTACTCTAGCAGAACTAGAATCATTTAAAGAAATAACCACTACTTGATTCAATGGAATGAAATCAGAATGAGCAAGAGTACAAGAAGCTTGAAAAGACCAATTAAAAGAAATCTCAGCAAAATAATATAAATTAAAAATACAAAAAATAGTGCTACTAATGGGCACTATTTTTAATTGTCAAAATTTAAATTCAAGTGCCTCACAACTAAATTTTATTTCAAATTTGTGTCGCACTTGATGTTTCATTCTAAAAACAAGATAGTTGCTAAATTTTAACTATCTTGTTTTTAGAAAAAATAAGATAAATAAATGATTAAAAAAATTAATAAAAAAAATGGGGCGTTTGATGGGGATCGAACCCACGTATGCCGGAGCCACAACCCGGAGCGTTAAACCACTTCGCCACAAACGCCATTTTGTTAAATTGCTTATATATTTTAACATAAAATACAAATATTATTGTAATTTAGTGCCAAAAAACTTAAACTCTTACTTTATATTTATTTTGATAATCCTTATTAATTAAACAAAAAAACAAGATAATTGCTTTCGCGTAGCTATCTTGTTTTTTAAATTTGTTTACTTATTTGTAAAATACAATGGTTTAATAATGTTTGAAAGTGCTTTCTGTCAAAGATTGAGCCGTTGCTAGTTCATTTTCAATTTCCCTGGTTTTTTCTTTCATTAATTTATCATCACCAATTTCTTGATTTAAATTAATTGATACCAAGGCATTTATTGCTTCTTTAGCATCTTTAATTCCTCTTTTAAAGACTTCTTCTTTAGTATGATTGTCAGAAATCAAATGGTTAAATTTTTTCCAATATCCATAAAACAATTTAATTAATTCATTTAAATTTTTTATTTTGCTTGTTTCTGCTTTTAAGGCTTCCAAACTTTCTCCTTTTAATGCTGAATTATATCCTGCTTCAATTATTTCTAATGCATTGTTCATTCATGATTGAAGGTTTTTGAATCCATCAAGTTCTTTAACTGATTCAGGAACTAGATTTTCAATTGATTCTTTAGTATTTTTTGTTTCATTTTTAAGATTACTAAATGCTGTAGTAAATTCCGTTGTTGGGTCTTCTTCAATCATTTTCTTTAATTTATTTTCTAATTCAGTTATTTCAGAATCACTAGGTATTGTTTCTTTGTCTAATATGTTTTCAAATTCTTTTACAAGTTTTTTTTGCAGAGACGATGACAATTTATCGTCATTTTCATATTTTTCTTGTAATTTGACAAGCCTTAAAAGATTTACTGAATCTTCTTCTATTTTTTTAAGATCGGCTAATTTCATATTTTTCGAAAGATTATTAAAAATATTACTGTTTAATTTTTCATTAAGTTTTGAAATAAAATTATTATTAAATTTTTTTGCCTTAAATTTTTTAAGTTGCTCTATATTTGAATTAATTACTGAAACTAAACCGCCTTTTATTTTAAGCATATTACTTATTCAAGGCTCAAATTGTCTAACATATTCATCTATCTTTTGAATTTTTTGGTTTATATTAGTAATTTTTGAATTTTCAGGAAGACCTTTTGCTTCTTGCGATAATTTTTTAGCATCTTCAAGTTTATTTTTTATTTCGCCTGCACTTAAACCAATAAGCTTTTCAATAGACATAAGGTCGTCTAAATTTTCAGTGTTTAATAACGCTTCTAATTTTTCGTTTTTTTCTTTTAAATTAGCCATCAATTCTTGTTTTTTAGCCTTGATTTCTTGATCTAATTTAGATTTTGCATCTTCAAGTGCCTTAGTTGCTTTTGTAATTTCTTCAAGAGTTGAATTTTTATCAATCTTGTTATTGTCTTCTAACAACTTTTGGGCATCATTTGTTCCAACTTCTTTTGCATCTTCTTCTTTAATTAAATCTTCTAAAGCTTTTTTTGCTTTGGCGAATTTTTGCAATTTTTCGGTTTTTTCTGTTTCTAATTTTTCTATAGCTTGTTTTAATGCTTCGTTTGCATCTTTAATTTTTTGAGTTGCATCTTTCAATGATTTTATTGAGGAATCATTGCTTAGATTTAAACCGTTAATTTCTTCTAATACTTTTTTAGCATTAGATATTGCATCTGCTGGTGCTTGATTACTTGAGGAGGTAATTAAATCACCTAATTCTGTTTTTGCTTTAGTTAAATTATTAAATTCAGCTTCTTTTTTAGCCTTGATTTTTTGTTCTAATTTAGATTTTGCATCTTCAAGTGCCTTAGTTGCGTTTGTAATTTCTTCAAGAGTTGAATTTTTATCAGCTTTGTTTTTAGTTAATGCTGCAGTAGCTTCGGTTGTATCAACTTCTTTTGCATCTTCATCTTTAATTAAATCTTCTAAAGCTTTTTTTGCTTCGGTGAATTTTTGCAATTTTTCGGTTTTTTCTGTTTCTAATTTTTCTATAGCTTGTTTTAATGCTTCGTTTGCATCTTTAATTTTTTGAGTTGCATCTTTCAATGATTTTATTGAGGAATCATTGCTTAGATTTAAACCGTTAATTTCTTCTAATACTTTTTTAGCATTAGATATTGCATCTGCTGGTGCTTGATTACTTGAGGAGGTAATTAAATCACCTAATTCTGTTTTTGCTTTAGTTAAATTATTAAATTCAGCTTCTTTTTTAGCCTTGATTTTTTGTTCTAATTTAGATTTTGCATCTTCAAGTGCTTTAGTTGCCTTTGTAATTTCTTCAAGAGTTGAATTTTTATCAGCTTTGTTTTTAGTTATTGCTGCATTAGCATCGGTTGTATCGACTTCTTTTGCATCTTCATCTTTAATTAAATCTTCTAAAGCTTTTTTTGCTTCGGTGAATTTTTGCAATTTTTCGGTTTTTTCTGTTTCTAATTTTTCTATAGCTTGTTTTAATGCTTCGTTTGCATCTTTAATTTTTTGAGTTGCATCTTTCAATGATTTTATTGAGGAATCATTGCTTAGATTTAAACCATTAATTTCTTCTAATACTTTTTTAGCATTAGATATTGCTTCAGCTGGTGCTTGTTTAGTAATTAAATTACTTAATTCTGTTTTTGCGTTAGTTAAATTATTAAATTCAGCTTCTTTTTTAGTCTTGATTTCTTGATCTAATTTAGATTTTGCATCTTCAAGTGCTTTAGTTGCGTTTGTAATTTCTTCAAGAGTTGAATTTTTATCAGCTTTGTTTTTAGTTATTGCTGCATTAGCATCGGTTGTACCAACTTCTTTTGCATCTTCATCTTTAATTAATTGTTCTAGCGCTGTTTTGGTGGTATTGTATTTTTCTAATTCTTGTTCTTTTTGCTTTGAAATTTTATTAGTTAATGATTCAAGAGCATTTTGGATTTTTGTTATTTTTTCTTTTATTTGTTTAATAGAATCACTATTAGTAATGTTGGTATTGCCGAATATGCTCAATTCTTTGTTTTTATCAACATTATTTGCATCTGGTAAGGAAATTAATTTTTCTAATTCAGATTTTTTAGTTTCAAATTCTTTAGTTAATTGTTCTTTTGTAGTGTCAATTAATTTTTGAAATTCTTCTTTAGCATGAGTTAATGCTTCGGTTGAATGTTGAATTTTTTCAATAGTTGCAGTTTTATCTACATTGTTTTCGGATAAGGTTTTTTGAACATCAGCTATGCCCACTTGAACTGCGTCTTCTTCTTTGATTAAATTTTCTAATGATGCTTTAATATCATTATATTTTTTAAATTCTTCATCCTTTTTATTTTCTATACTTGTAGTCAAAGAATTAATTGCTTCTGTAATTTTTTCTGTTTTGGCTTTTATTTCTTTTATTGGAGTATTTGAATCAATTGAATTATCTTTTAAAACATCAGATTCTTTTTTATTATCAACACTTTTGGCATCACTTGTATCTAGTAATTTGTTTAATTCATCTTTCTTTTCATTGAATGTTTTTGTTTCTTTATTTTTAGTATCAATAATATTTTGTGTTAATTCGCTTTTAGCACCTTCAAGAGCTTTAGTTGCTTTTGAAATAGCATCAAGAGTTGAATTTTCGTCTGCTACATTAACTTTCAATGCTTCTTCAGCCTTAGAAGTTCCAACTTCTTTTGCATCTTCTTTTGCAATTAATTCTTCTAATTGTTGTTTTACTAGATTAAATTTTTGTAATTCAACAGCCTTATCTCCTTTTGCTTTGGCTATTTTTTCTTTTAAGGTTGATATTGCATTTTCGATGTCGCTTGTTTTTTGTTTAATTTGTTTAATTGTAGAATCATTATCAATTGTAGTTTTATTGTATTTTTCTTCTTCGGCTTTTTTATCAACTTCAGATGTTGTAGAATCTAAAAGGCTTTTTAATTCTTGTTTCTTTGAATTAAATGTTTCTTTAGCTTGTTGTTTTGCACTTTCAATGCTACTTTTTAATTTTGTTTCTTCGTTAGATATTTTTAAAATAGCATTTTTAATTTCTTCAATAGTTAACTTTTCATCAACTTTTGTTTTTAAAGCTTCGTTTGCACTATCAATGCCTACTTCTTTTGCATCTTCTTCTTTAACTAGTTTTTTTAGACTTTCTTTAGCCTTTTCAAATTCTTGAATTTTTTCGTTCTTTTTATCTTGAACTTTTTTATTTAAGGTCTTAATTGCTTCATCGATAGTTGAAGTTTTTTGTTTAATTTCTTTAATAGAATCTGTTTTAGAAATTGAAGTATTAGTTAAAACATTTAATTCATTATCTTTATCAACATTATTTGCATCACTTGAGCCAATTAATGTTTTTAATTGTTCTTTTTTAGTTTCAAATTCTTGTTTAGCTTTTTCTTGAGCCTTTGAAATTTTTAGTTCCAATGCTGCTTTTGCTTCTTGCAATGCCTTGGTTGCCTTTGTTATTTCTTCAAGAGTTGAATTTTCATCAGCTTTATTATCTTGTAATGCTTTTTTAGCTTCGTTTACTCCGGCTTGAATTGCATCATCTTTTGCAATCAATTCTTCTAATTGTGTTTTTATACTATTAAATTCAACTAATTTGGCTTTTTTGTCTTTTTCTTGGGCAACATTATTATTTAATTTTTCTATAGCGTCCTTTGTTTTTATAACTGCATTTTTTAGTAAATTAATTGGTGAAGAGTTATTTAATTTTTCAACTTCTGCCAATACTTTTTTGGCTTCTTCAATTGCTTTTGCTGACGCATCAGTTGATTTGGCAATTAATTCATTTAATTTGCCTTTTTCAACTGTTAAATTATTAAATTCAGTTTCTTTTTTAGCCTTGATTTCTTGATCTAATTTAGATTTTGCATCTTCAAGTGCTTTAGTTGCCTTTGTTATTTCTTCAAGAGTTGAATTTTCATTTACTTTATTATTATTAAGAGCTTTTGATGCATCTTCTGTTCCAACTTCTTTTGCATCATCAGTTTTAATTAATTGTTCTAAAGCAACCTTGATATCATTAAATTTTTGCAATTCGTTTTTTAAAGCTTTAACGTCTTCAATTTTTTTGTTTAAAGATGATATTGCTTTTTTAATTGCTTCAGTCTTTTCTTTAATTTGTTTTATTGTATCTCTTTCGAGAATATAAGTGTTTGCCAAAATACCAATTTCAGTTCTTTTTTCAATTGGTTTTGCATCTTCCGAGCCTACTAAAACTTCTAGATCTTTTTTAACTTTATCAAATTCTTTTATTGAATCTTCTTTAGCACCATTTATTGCATTTTTCAATTCAACGGTTGCATCTTTAAGAGTTTTAATTGCTTGGTTTATTTCATCTATTGAAGAAGTGCTTGTTATTTTAGTATTTTTTAATGTTTCGGATGCTTTATTAGTATCAATGAATTTTGCATCTTCTGAATTGATTAAATCTTGCAATTGTTTTTGAATTTTTTCAAATTTTTCTTTTGCATATGAAGGATTTAATTTATTTTTTTCATCCATTTTTTGGATCAATGAATCAATAGCACCTTGAACTTCTTTTGTTGCTTCCTTGATTTCTTTTATTGAATCGCTATCATCAATCTTTGCATTATTTAAGACTGTTGTTTCGTTTTTCTTGTCAATATCTTTTGACCATTTTGATTCTAGCAAAGTCTTTAATTCTTGTTTTTTTGCTTCAAATTCTTGTTTTTGAATACTCTTTTCATTTTTAATTGCTTCTTTAAGTTCTTCTAGAGCGTTTTCAAGATTTATTCTTCTTGTCTTAAATTCTTCAGATGATAATGTGGTATCCTTTGTTGTATTTTCTGCTAATATCTTTTTAGCTTTTTCGTTGGCACTTTTTATCATTTGAGCATCTTCGGTTTTAATTAATAGTTCCAATTTGTTTTTAGCGATTTTATATTTGTCTAATTCACTACTTTTATTAATTTTTTCTGCTTCTGAGATTGCTTGTTTTTTAGCATCAATTAATAATTTAGTTGCTTGTTGAATATCGCTAATAAAAGAACTATTAATGTTTATATCGCTAACTTTTTCAATTGATTTTTCTGCCTTCGCCTTAATATCTTTGTATTTTAAATCCTTTAAATCGTTTGCAATGTAATCTGTTAATTCTTGCTTTATTTTATTAAATTCATTAAATTCTTGTTGCTTCTTAAATTTAATTGCTTCTTGCAATTCTTTTGTAGCTTTTTTAATTGTTTGAACTGCTTGAAGAACTTGAAAGGCAGCAGATGAATTTGTTATTTTAGTATTTTTTAATACTTCAGCGGCCTTGGTGGTATCTACTGATTTTCCGTCTTTTGAATTAATCAATGCTTGTAGTTCTTCTTTGGCATTTGCAAAATCCAATCTTAGTTTGTCAAGAGTTTTTTTATCATTTTGATTTTGTTGTTCTTTTTGTTTTCTTTCAAGAATTTTTTGAGTTAATGAATTAATTGCTTCTTTTACTTGCTTAGTTTTATTTTTAATGTCTTGAAGCGAAGAATTTTCATCAATCTTAGCATTATCTAAAATTTTAGATTCTTCTAATTTGTTAACATTTTTAGCATAATCTGATTTTAACAATTTTTCTAAAATAGATTTGGCCTTATTTAGTTCGGCTTTAGATTTTTCAATATTTTGCTTTAATTCTTGTTGCTTTTTAAATTTTCCTTTTGCAGCAATGGCTGCTATAGTAGCAGTTGTAGCTGCTGTGGCTAACAAAGAAATTGAAAGTAAAATACCTATTTTCTTATTCATGTTATTTATTTCTCCTTATCCTATATAAACTTAATAAATAATTTATTAATTTGCTTATATTAAGTTATTTTGGAGCAAGAACCCTTAAAAAAAAAAAAAAAAAACGGTTTTCTTCTTCTAAAGAGATTTTTTTGAACTTATTGGTTAATTAAATGTGATTTTTTCTATTTTTTTGAATTTTTTTTTAAAACAAAATAAATAATAAATATATAGATGTTTTTTAGTATGAAAGCCCATGAACATCTATAACAAATTAACTATAAAAATTTATAATTTAGAAACTAAATAAATCATTTTTTAATTATTCGAAATAATAAAAAAGGCTTGCTATTATTGAATGCAAGCCTTGTTAATTTTTTTAATTATTATTAATATCTTGGAATTATATGAACATGAATTCTGAATACTGTTTGATCTGCTGAAAGATTATTATTTATTATTAAATTAAATCCAGTTGCGCCTAATTCCTTAACTCTTTTTAGAGCAAGTTCTCTCGCCTTTAATATTAGTGATGACAATGCTTGGTCATCAATATCATATAAATTCGTGGCGTGGTTTTTTACCGTTACTAAAAAATGGCCCTTATCTACTGGTTTAATATCTTCAAAGGCAATAAATTTTTCATCTTCATAAATTATTTTTGCTGGGATTTCTCTTGTTATTATTTTTTCAAAAATATCATCATGCATATTATACTAGACCTTCAACTTTCAATATATCATAAACAGATTTGTTTGTTTCTGAAACATTTAATTTAAACCCTAGTTCATTAATATATTTAACTGCAGTTTTATATAATGACTCAAAATTAGCAGTCACTATATTATTTGCAATAATTTCTTGTTTTTCTTTTGTTGCTCATGGAGTTTCATCAAATGTTAAAACTTGTTTTGTAACTTCTTTGCCGTTATAATCTTTAACTTTCTTAGTTAAATATAATGGTGAAATTTTAACTACATATTGGGCAGTAATTGAATTGTCTTTTGTAACATTTATTCTTTGAGGATTTTGTTTTAAATTGTATGCAACATTTTGACTTATTGACTCAAATTTCTTTGTACTTTTATTGTATTCTAGTTTAGCATTGAATTCATAGAAATTTCTTTTTCCTAAACCATCAACTGTTAAATTCATTGGAATTGTTAAAAAGTTACCATCAAATGATATTGAACCTTTTTGGCTAAATCTCAATGTATATTGCTTACTACCAAGAGTTATTGTCTTTGTATATTGATAATTATCCTTGCTATCTTTTGTTAAACCGTCAAATTCGTAATCTTTGTAATCAAACGATTTTAATTCACCTTTTGAAGCTTTAGTTTGCAATGTTTCGGTTGCTTTTGCCTTTGGATGATTTATTTCTTGGGCAAGAATTTTTGCAAGTAAAAAGCTTTCTTGACTATAAACTTTTTTACTAAATGGATCTACAAATCCATATACTGAACTTAAATCTTTCTTTATACTATCTAAATTGCCGCCTAAATCACCTGACGTATTTGAATTTTTAACAATGCCTTTATATGCCAATAAAGATTTTAATACTTCGCCTTCCATTGAACCGCTATTAATCAATTGTTCTGGGTTTTTAGCTGCTTCATTGTGGCCATATTCTGGTTTAGTTGAAGGGTTGTAAGAAGCTAGTTTATTAAATCCTTCAATTGAATTTATATATGATTTTGGTCATCTTAAATTCATATCGCGCTTATCATTAAATTCTCAATTTTCAATAATCGGATTTTCAACTAGATATTTCATTAAATACAATGAATCATCTGCAAAATTCAATGCTTCGTATAATTCTTTTTCATTAATAGGAGCATCTTTCTTAGACATTTTGTTGTGTAATGCAACTTGTGCTTTGTCTAGACCGTTGCTATCATTTGCTATTTTTTTAAGTTCTGGTCTATTTTTTAATAAATAAAGTTTAGCAAGTAACATTTTTTGAAGTTCTAATCTAATGTTTGCTGCGATTTGACTTGAATGTTTAAAAATAAATTTAAATTCTTGTTCACTTGGTATTGAAAATGCAGCGGGATTATATTTAGTTGTATCAATCTTTGCATTGATAAACAATGATTTTAAATTTCAAAAGAATTGAGGATCGGAAGCAACTCTATTAGTTGCATAATATTTGAATAATTCATAAAGATCTTTTGTTAATGCTGAATTGCTGAAATTTGAAACAATATATTCAATTTTATCTTTATCTTTAAAATTTTTAGCATAGCTTTCTATCTCACTTTTGTAAAATTCTGAAATATATATATCAGTAAGTCAAGTTAAAACCCTATTTTTTGTTAATGAATCTTTTAAAACACGTTTTTGGTACCCTGAATTTTGTTGAGAATCTTCCTTTTCTTTTTTACATGAACTAGCAATCAAAGGAAGAACCGCAAGTGGCATTGCAATAGCTGTTAATAATCTAATTTTTTTAGTCATTGTATCTCCTTTTAATTTTTAGTATTTATGTAATTTACAATTGTTGTGAATAAATAAGATAAGATCTCTTTGTCTTCATGGGGCGATAGAATAAACTTATTGTCTTTGAACACATAATCTTTGTATAAATTACTATTAAATTTTTTAATTATAAATTGTTTTATTTCGTTTGCTTTGCCATTAATTAAAACGTATTTGCTTGTTTCACGAATTAATTTTTCATAGTTGATTGCATTTAAAATATCTTGATCATTGAATTTACGTTTTGTTCCTTCAAGACTTAAATATTCTTTTGATTTAATATAATTTTTAAAATTATCTTGTTGTAATAAATCATTAACCATATAGTCACGAGTTAAAATTTCAGAAAATATTGAATCTAAATTAAATAATTTAGTATTCAATGTATCTGAGTGGTTGGCCTTAGATAGTATTGACAAATCATTTGTTATCAATCTAGTTACAACTTCTTCTTTGTCTAATGAAAATACATTTTGAATCAAAATTCCACTTTCATTGACTTGAATGTAATTTCCATTACTTTTAATTAATGTTGAAACCTTATATCCACTTTTTTCATCACTAAATGCATCTCTAAATGCCAAGCCAAAAGATTTATTAAAATCTTTTTCACTTAAATCATTGATAAATTTTTCAATAGCTGCATTGTATCTAGTGTATTTTTCAGAGAAGTTTTCTTTGTCAACACTTTCAGTTCCTTCATTAAAAATATCAGCAAATTTCATTGTGTTCTTTTTATGATCAATAAATGTAGCATCGCCAAAGATCTTTTTGAAGTTTTCTTTTAATTTTTTAAACAAATCTTTTTTATCTTCAGCTTTAAAAATGCTTTTATCTTTGTCAGCATCGCCCATTAAGATTGAAAGAAGATTCATATACTTATTAGGATCTTTTTCTTTTAAACTATTCAACACACTTTTAAAGCCGTCGAAACCGTATTTATCAGCTGACTTGCTATCGCTTGAAACATATCTCAAAAGGTTGCTATCATTTTTGGCTGCAATTTCTTGATTTTTAGTAATTTCATTTGTTTCAAGAAGTGAAGATATGCCCTTAAATGTTGAAATGGCATCAATGCCTTGTTTGATTGAAACCTTGTCATCACTTTCATTCAAATAAGCAGAAAAACTTAATAATTTAATAATTTCTGCTTTTGTTACGACTCATGGATTTGAAGCATCTTTTGGATCTGGATGTGCCAATAAATTTAAATTAGATGTTATTGCTTGCTTTCTTTCAATTCAATTTTTAATGAATTTTAAAGGATTGCGATATTCCTTGACAAAAGATTTTGTAACAAACATCGGAATTTTAACTTCATCTTTTGTTCCAATTTTTAACGATTTATCGTCAGATTTTGGCAAGACATAATTCTTGTTTTCTATAGCAAAAGGAAGAGTAATTTTTTCACCCTTCTTAGCAATGTCAACTCTTTTGCCTTGATATGTGTAATAAACGTCTTTGTTTGCAATAATTCCGTTTTTTAATTCACTATAAGTAAAATCACTATTTACTTCGGTTGTATATCTATTAAATGCATATCTATTTAATTTTTGTGCTAGAAGGTATTCAATTGCTTCTTCTTTTGACTTTGCTTTTCCATATCTATCAGTTGCAAGTTCTTGTAAAAATTTTTCTTCTCAATTTTTTTGTAGACCAAATTGTTCTTGATATTTCTTTTCAAGATCTTGAATTTCTTTTGTTATTTCTTTTTTAACTTCTTCGATTGGTTTTAGTGCTAATTTTTTAATATCTTTTTCTGCCTTATCAGCATTATAAACAGCTTCATATCACAATGAACCTTCATATTCTTTTTCATAAAGGTATTTAATTAGTTGCTTTTCAACACCTTCAGCAGCATGTTTTGATGAATTTGCTATTTCATGGATTTTATTAATGTCACCATAGTTTAAATTTAACCTTTGACCATCTGGCCCGGTTATTGATAAAATTGAAGAAGAATCTTTTAATAAAGGTGAAGGCTTAGGTAAGGAATGATTAGCTTGTGCTAACGGAACGCCAATTCCTGCAGCAACTGCGGTTGCTATTGCTGTGCCTCAAAAAGCACCTCAAATAATTTTTTTACGCTTAGCCTTTTGTTCGGTTGTTTTGGGTTGATTTTGATTTGACATAGTAAACCTTTCTATTATTTAATATTAAATAATTATACTATTTTTGGTTATTTTTCTTTATTTATGGATATTTAACCAGTATTATTTTATAATTTAAAACATGAATAAATTACTTTTAAATAAGGATAGAATATTGCTTGAATTTATCGAAATTTGTAAAAATCTTAATCTTTTTTATAGTTTAGATAAATTGAGCTTGTATGGCACTATGTCAAAAGAACATTCTTGGAATAATTTGGATCACCACGAAATAATGATGAAATATCAAGATTATGAAATCTTAAAAAATGCTTGTCCTAATAGAGTTTTAGATAATACATTACATTCTTTGTATCATGACTTACAAATAAAATTTGTTGATGATAACAAAAATATATTTGAAAACCAACCGTTTTTGAACATTAACTTAATTTATCCAACAAACATTGCCAATATAAAGAAATATTTAAAACATTGCAATCAAGCTAGATCGTTTATTGGCCACTATTTAACAATGACAAATAGCAATGATTGAAAAACAAAAAATAATATTATTAAATCTCATTTTCTATCAATCTTTTATAAACCAATTACATACAAAGAAGTTGCTAATTTATTAATAAAAAATGATCACCAAGGTTTTTTGATTTCAAGTCCGATAATTAAAAAAGATTCATTGAAATATTGATTGCCAAATATTAAATTTGAAACAAAAAAAGTAGTTATAAATAATATTGAAGTTGATGTCTTAGTTGAATTTGACCAATATCTAAAAAACGTTTATGAAGTTGAATATTTTGAACATCCCATTCCACAAAGAAAGATGGAATATATTAATCCCATTGAATATAAAAAAATATTTAGCTAATTTTTTATAAAAGAGAGTTCACAATTGCTTTTTATTGAAGAAATTTTAGAATTTCTAATGTGTATTGAAATGTCTTTGTTTTTTAAAAAATAAGCAAATAAAAAAGTAGGAAGTTTCTACTTCTTACTTTTTTAAAATTGACTTTCTCTATCGATTAATCATTTTCTTTCTTGAAGTTTTACTTCTTTAATTAATCCCAACTCCAAGTATATAACTTTATCAGCAATTTGGGCAACATCAGGGTCGTGGGAAACAATGATAATGGTTGTGCCATATTTTAAATTGATTTGTTGCAAATTTTTCAATACAATAATTCCAGTTTTTTCATCCAATGCTCCTGTTGGTTCATCAGCTACAAGAATTTCGGAATTTTTTGCAATAGCTCTTAAAATTGACACTCTTTGTTGTTGACCACCAGACATTTGAGAAGGATATTTATACATACATTCAGAAAGATCAAAATCTTCAAATAATTTTTTAATATTTAAACGCTTATTTTTATCTCTTTGCAAATATGAACCAGTTTCAACATTGTCATAACTATTCAAATTTTGAAGTAAATTATAATTTTGAAAAATGAACGAAATGTTTTCTCTTCTAAATAAGGTTTGCTTTGCATCAGATAAATAAGGCAAATTTATGTTATTTACAATTACATTGCCAGAGGTAGGTCGGTCAAGAGCACTAATTATGTTTAATAATGTTGATTTACCTGACCCAGACTTTCCATATAGAACAACAATTTCTCCTTTTTTAATTGAAAAAGTTATTCCTTTTAAAACTTCAGTTGCTACATTTCCAGATAAATATGTTTTTTTAATATTATCAACTTCAACAATGTTATCGTTAGAATTTTGTAATTTAGCATACTCGTCTTTTTTTCTTGGTTTGTCATTAGCAAGTCTATATTTCTTTAAAGTTGCATTATCTAAAACCTTAATTGCAATGCCTTCATCTTTAATTTCAAATGGCTTTTTTTGCTTAGAAATATTTATCAAGTTCAAATCTTGCAATGTTTGTTCATCAACAAAATTTTTATTCTTAGAAATTTTATTTGTTTTAGCTTTAATTTCTTTTTTGTTTTCTTTTATTTCTTTTGCCATTTTATTTTCCCTTCAAAGCATCTACTGCTTTTATTTTATTTAAACTGTTTCATGTTGCAATTGATGTTGCTATAAATACTGCCAAACAAATTAATGCACTTAATATTACTGTTGAAATATGTAAAACCAAAGGTAATACAATCTGGCTAGTTACCATCATAAACGAGTTGAATATTGCTATTATTGCAAGAGTTGCAGGAATCATTAGTAAGCAAGCAAATACAATTGCAGGTACAAAATTAAAGAAGAATAATAGAATTTTTTCTTTATTTGTATATCCCAATATAGAAAATGTAGCAATTGATCTTCCATTCGAAGCAATCATTATATTAGTAATAACAATTAATATAACTATTGAAATTATCAATGAAATTATTATAAATGCCAGCAATATGGTATTAACTGTTGAAGAAATTCCTTCAATAAATCCAGCTTCAATATCTTTGCTATTAACATCAAATGATGCACCATACATTATATTCTTGCCATATATTGAAGATGCTTGTGTGCCATAGAATTTTGTTAATGTTTCTCTTACTAAGATTTTATAACTTTCTAATGGACTGTCTGAGTCTATTATTTCTTGTATACTTTCATCGTTTAGATTTCCAAGTAATTTCTTTAATTGAGTTTTATAATCTAATTTTGCCTGAGGATGTGCTTCATTATATGATTGAATATTATGTTCATATACAGAAATATATTTTAATTTTGGATTGCTTATAAACATGCGTTTAAAGAAATCTCAAGCGCTGTTGTCATTTGCTTCGGCAACATCATATGAACTTGCTGCACCTCAGAATCCTGTTGCTGAATAAGTTGTTAATGTATCAATAGTTTGAACTGGAACTTTATCAATTGATAAAATACCATTAAATGCATCATATTTTTTATTGAATTTTTTAATATATTCATCTTTTAAATCAGGGTGTAAACTAATTGCTTTTTGCAATTCATATTTTCTTGCATCTTTTAATCTTTTTGAAAGAGTATCATAGCCTAAAATGTGGTCTAAAATATCTTTTCTTGTACTAATTTCAGAGTTAATATAAGTATCTGATATGCCAATTACTTTAAACTTATAATCAACTCTTGGACTTTTTTGCATTAATGCTTGATATGCAAATCTATCAACGTGATTTAATATTTTGGCATCAAATACGGAATTAACTTTTAATCTATAGCTTCTTGCTGTTACTTCATTTACAACAACAGGAATTGGATCATTACTATCAAAATTGTAATTATAGTCTTGTAATAATTTTGTTAAATTATTGCCTCGACTATCTTGCAATTTCATGAATTTACTATCATTATAATATCCATAAATTTTAGTTTCTTTTCCATTTAAAGTTACTGAGGCGTATGTGTATTTTTCGTTAGTTGTATCGTTTCAATAAACTCCACCAAATGAAACATAGAAATCTATACTATTTATTTTTCTATAAGCATTTACTAAGAAATTACGATATTCTTGTCTGAATTTTGCTGTACTTACTTTGGCAGGTTTTAAATAAATTTCATCTTCTGCAACTCATTCAACAAATCTAAATTGTTTTCTATTTCCTGATGCTGCTTCTGAATAATTACCAGCGCTAAACAAGAAGTAACTTGTTCTATTTGATAAATCTTCTGGACGTTTAGCATTATAGTCATCAAAGAATTTTTTAATATCTTTTACAGCAATTATTTTTGTTGGATCTTTTACATCTTCTTCATCTATTATTCTATAATCTCCACCATTTTTCAATCTACGTAAATTTATTAAGTTTTGAGTTGATTGCATCATTGAACTTACGCGGTTAAATATTTGGTTTACTCTTGCCCTTTGTGTTTCGGGCATGTTGGCATAAGTTATATCTCAAGGACTTAATTCAACTGATAAATCCATTAATAAATCAAGTGAGGATTTTGTAATGACAGTAGGCGCAAATTTTCTTTGAATTACATCAGTATTAAATGAGGCGCCAGGTCTTAAGAAATTAGGATTATCATAATCTAGTTGACTACCATTGGTTGATGAATCACCTGCAAGATCATTTGGAACATACAATAAATCATTTATATCGTTTTGATTGTATGTAACATAAGGACCACCTTCGGTTGTTGGACTTTCAAGATTTAATTTATATTTATATAGCCTATTTTTATATGTTTTTGAAATAGTTTTATTAAATACATTATTTGAGGATAAGAAGAACATAGAAATTAATGAAGTCGTTGAAAATGCTAAGAATAACGAGAACATTTTTCAGAAATTATTCAATGCCATTGAGGCAATAAATCTTGCTTTAACTGGCATTTTTCTAAACATAGCAGAAACTCTTTGGGCAAAATTACCAACACTAACTTCAGTTAACCCACTCATTAATTCTGTAGGTTTATTTCTTACAATAACTCTAGTAATTATATAAATAACTAGTGAAACAACAATCAATGGAATAATAACTACTGCTAATATTAATAATATATTAAATGGAATAATATTGTTCTCTAACGTTCAATAAGAAGATAATATTGACATTGCAGGTTTTTGTAAGGCAAATCCCACAATATAACCAAATATTCCTCCGACAATAGTTGGAATTCAACCAAAAGCACAGAATGATAAAGCGATTTCTGATGTTTTATATCCTTGAGCTCTCAATATTCCAACAACCTTATTTCTTGCTTCAATATATCTTTTGATAATAAAGTAAACAATCAAAATTACTAATATTGCCAAAATTGTTATTAAATAAAATGTTGCATTTTTAATTGATGTTACTAATGTTCTTATTGTTTTCACTCTGATGTAACGTTCAGGGTTGATTGCATCTGGTTCATCTTTTAAAAATACTTTATTTTTTGAATTTGGAGAAATTGATGAAACAATTCTTTGAAGTTCTTTCTTTAAGTTTTTAGGATCTTTGCCAGCTAAATAGTTTCCTTTTTTGTCCACAGGAGATTTTACTAATGCATAACTCTTTAAGGCAAATGTAGGATATGCCGATCTAATTCTGTCAAAACCTCTTGAATTAACATAAACAACAGATTGAGTTTTTGTATCAACTTGTAAGTTTTCTTCATTAATTACAGGATATAAATAATCAGCGGTACTATCAACACCTGTTATCAAATATTCTTGGGTATTTACTTTAATTTTATATTTATCATCTAATGAAGCAATGAATTGTTGCATTTCAAATGGATTATTTAAATATTTCGAAATATCGCCTTTGTATACTTCTTTTTTATTTTTGTTTAAATATCCATAATTTACCTTGGCTAAATATGAAGCAGCTTCGGAATATGCAATTAAATTATAAACTGTGCTTGGCTCTATTATTTTTGAAAATACTTCCTTAACAGTTTTGGTAATATTTATTCCAAAAAAGCTAATATCTTTGTTATCGGGTGTTTTTAGTAAATGGTTAAACATATAATTGAATTTAGAAATATCTAAATCAATTCTGCTATCGCTTTTTGAAGTAACAACATTTTCAACAAAATTGAATTTTTGAGTTGCACCTTCGATTTGACTTTTCAACATTGTTGCATTAGTTTCATCAATATTTGATAAGAATTTTTTGATTATATAAGCAATATTTCCACTATCTGGGTTTGGACTTAATACGTAGTATAACAATATTTGTGGGAAACTTAAAACATTGAAACCATTTAGAATATTTCTTGGTACGTTTAATAGCGAAATTATTGAGGCGGTTTTTGATGCTGCAATTGCATCCTTATATACTAAGCTAAAGGTATATTCATCATTTATTAACGGTTTAATTTTTTGAGTTTGATCATTGACATTAATAAATGATTGTAAAAATAATTTTAAGGCTTGAAGATAATGAGTTGGCATTTGATTTGAAGAGGCCATATAACCAAAATTGTAGGTTATTTCATTCATATTATTTTTTAATATCTCTTTAATTGCTTCAATGCCCTTATTAGAAATATGATTTTTAGATAATTGGTCAAATAAATTTTTAAATACTGCTGAATTATTCATTAAATCCATAATTTGTTTTGTTGCTTTTGCTGTATGTGCGTGAGCAAAAGGCATTTTAAATAATTTAGCAGAATAGTCATCGTCCGAGCCTAAAATTCCAAGACTAGGAATTGGGTTAATTACTTTTTTGTAATTTAATACTATATCTTTAATTTCCTTTGATATTCCTATGTTGTAAAGGCCTAATAATATCTCTTTAATTTGCTTGTAATCTAAATATTTTGTAACTTTCCGTTCAATTATTCTCTTATTAGTTGTTGGATCAATAACAATATTTTCTTGAATATCTGCAATTTGGCTTAGTTGATATGCAAGTTTAATTCATAATGAATATAGAGATAAGACATTAGAAGCAACATTTTGTGGATCATTATTCAACATTAATTTTGCTAAATTGTCTTTTATTAATGAATGAATAGTTTTTAAAATTTCCAAATCTTTTGCATGTTCAGGCGCTATATTATTAAATGAAGCTGAATAATATGCAACATCCAAATAAGATTTTAAATCATTTAATTCAACTGCCGAATTTATACCGCTTTGCTTATATTGAGAGTTTGAAGTTACAAGTTTGTCTATATATTCATGATATTTAGTTAATTTTGAAATTATTAAATCAAGGTCATTAAATTCGTCTTTGGTAACTAAAACATTGTTTTTTAATACATCAAATTCGTTTTGAGTTAATTTCAACTTTTTGTTATTTATTTTTGCATCTTTGGCCTTTAATAATATTTTTTTAATATCATCAACATCAAAAGGATTTATTGGAATATTAGTCAATACATTTGTTTGCTTTTGAGATTGAGGGAATGAGAATTTTGACATGTTTATTAAATCAAACAAAGTAATTTTATTATTGTCATAGTCAGGAATAGTTATATTCAATTTACCCAAAATTCTCTTTGTCTTATTTGAAAAATTAAACATTTGAATAACGGATTCTTGAATTTGGGAAATTTTTCCGCTATTTATTTTTCCATCTATGTTTGAACTTATTGCGCCAAAAAATGATGTTAAATAATCTGAGCTAGTTAAACTTTCTGTGTTGAAATCTTTATAAATTTTATTATTTGCAACAATTGGATAATGTTCATGTTTAACTAGTTGCTCCAAGATTTGAATAAATCTATCAAGATCAAAATTAGCTATTATATTTTTTAATCCTTGTTTAAAATTATCATATTCTTGGCCTGCTTTTGTTGCACCGTTTAATTTTCTAAAAAATTCTTTAATTCCGTCTTTTGTTTGTTGGTCTATAAAATTATTTGCTTCTTTGTTTGCATTAATTCATTTTGAATACAAACTATTAGGCGATTCTGGATTTAAAAAGCCATTAAAGTCAATGTTGTCAATCAATTCAGACAATGTTGATTTTAATTTGTAAACATCAATAGATTTTAAGAAACTTATTAAAATTCTTTCCGATGATAATATTCAATATGTATCATCAACACTAGTAAAAGGTTTGTATGGGTGTTTTTCAAATCAATCTTGAATAATTAAAGAAAATTTTTCAAAATCAATAGAGCGAATAACATTTCTCAATACTTCAAAAATTGTTGATTTATTTTTAACTAAATTAATTATGTCAAATAAACTTAATTTCAAAGAAGGAATCAAATATAGATTATTAAGATTTAATAGTTTGCTTATGTTGTAAACTTGTTGCATTAAATATTGATTTCGTTCATTGTCATTGGTTCCTGAATTCATATACATTTTTTCTAAATAGTTTATTATTCCAATGAATACATTTTGAACAAAAGCATTAACATTAACATTATTAATATTTAAATTGTTACTATTTGTTCTATCTACTGGTCTAATAATGTATTTTAAAATATCTAAAAGAACTTTTTGTAAAATCGTGCCATTTACCTTACCAATATTTAGCAATGTATGGAAATCATTATCCACAACACTTTGTTTTGCTGCATCCACGATTCTCAATAAATCGTCTTTATTGAACATGTTAATAATTTCACTATTAAATAAGGCATCTCTAATAACATTAACTAAACCATTTATTGTTTTATTTTGAATAATTTCGTTAGTTAAATTGTTGTTTATTGCCCCGAAACTTATAGGCAAACTAAATGAGTTTTTAAACTCCTTATCAGCAACTGCTCAACCATTTGGGCCGATTTCTCCTCTAATACTATAATTATTTCTAATCATGTATTCATAAAGTTCTTGAGATGTTAAATATGCTTTTCCATTAACATCAACTCTATTTCAAACTTTTTTATCACTAAATCCGTCAATAGTTGAATATTTTAATAAAATGTATTTTTCATCACTAATTCTTGCAACTGCACCAACTATTGAAGAACCAACGGTCTTTGGTTCGTCATTAGCAACAGTCAACATCAATATTTTTCCATCAATTTCATAAGGTATTTTTGTGTTTGGCAAGAAATCATAATATTTTAGAAATCTGATATCTGCATTGAAATAATTAATAGATGGCGTTCATTGCTCAAAAATATATTTAATTATTTGTTTTGTATAAACTGGTGGAATTTTTTTAATCATTGGATCGCTTGGATCTGGTTTCAAAATAAATTCATTAACATTTTTATCTGATACAGAAGAATTTAGCAATGTAGGATTTAGAGTTTCGTTATATAACTTACCAACGTTATTTTTTACGCCATAAATTTTTTGGTTTCCATCGCCTGCATTAACAAAGTGAAAAACGTTTTTCTTAGTAGTTTTTTCATCAACGGATTCTACAGTTAGAGTTTGTCTAATTCCTAAGTTTTCTTCTCCAACATTATTTTTTATTTCATTAACTAAGGCATTTCTAGCATACGAAACGGATCCTTTTCTAATAAATTCTTGATATTTTTTTAGATTATTAGGATCTGATATTGCTTTAAATTGGTCTTCTGTAATTAATTTATCACCGGATTTAGAATACTCAATTTGTTGAATGTTTCAACGGTGTTCCTTAGAAATGTTTTCTTTTATAGCAGGAATTTTGGAATTATTAAATCCACCATTTCAACTGTTTTTAAAAGTTTGCAATAAATCTTTTTGTTCAATTGGCAATGTTGTAGTTTTTTCTTGTTTCAAAGAAGACGAATTTAATTTATAAATTGTTTTAATCGTCTTATTTCAATATGAAAAATATTGAATATTTTTATAAACATTTGGGTCACTTTCTTTTAATTTGGCAATGTATTTGAAATATGATCCTTTTCAATTTGAGCTATATTCGTCACTCTTTTCGTTTCAATTTAATGCAAATCTATATCAATTATATGTTGTTAAAACCTCGGTAGATTTTATTCATTCATTTGGAATTTGATATATTTTATTTGCTGCTAGTTTTGTAAATTCATTAACATCTTTTTCAATACTTTTATTACTTAAGAAATTTTGAAGTTTAAAAGTTCTTGAAATATTAAAGTCTTCATTAATAAATTTTGATTTTTGACTTGTAAAATCTGAATTTTCGATTTTTAAATCAGAGTTTTTATTATAAAGAGATGCATCAAATTCATAATGGTTTTCTGAAATTTCTTTAAAACCTAGCATCTTCATTACATCTTTACCATCAATAGTGTGCAATGAACCACTATTTTTTCACGCTTCATAATCTTTAGTATCAAATGATGCTTGTTTAGTTTTAACATTTAGAAATAATGGAGAGGGATTGTATAAATAATCAACTACTTTTTGATCGTTTAGTGAACCTCTTGGACCATATGCAATAGAAGCTATATTTTTTAATCTAACTTCATTTAAAAAAGTAATGTCATCATTTTTTGTCAAATTATATCTTTTAGTTAACGGTTTTATATTTCCTTTGCTATCTTTTATATAAAGTTTAAATTCCCTATCGTGACCTGAAAAACTAAAATTCCTAATTTTAGAAAAATCAGGGTTTGATTTTAAAATTTCAACGTCTACATCACTTATAGATTCCTTGCTAGAATATAATAAATTTAATAAATCATAAGTACTTATAAAATGGTTTTCATTATCATAATTTAAATTATTAAAAGTATTTTTTAAACCTACATAATTTCTTTGATCTTTTGGTAGATCTATTGAATATGAAATATCACTTCCATTATTAGAAGACTCAAAAACAACAGGTTTATCAACAATTGATTGATTATCAGATCCTATTTGGTTATAACCTGATTTTGGTATCACACCTGATGGGTTAACGTCAAAATCAACAGTTGCATCATGCAATTTTGAAACTTTTTCATACGAATTAATCGTAGATGAATAGCTAGTAGTAACATCATTTAACAAAGTAAATAAAAATGTTGTTAAGAAAATTAAAATTGTTAAACAAATCAAGGTAACTTTATTTCTTGCCAATGATTTGAAAACCTCTTTAAAAAGTCTTCTCATATTTCTTCTCCAGTTTAAAAAATATAATTTTTTAATATATTAAAAATATAAAAATATGTGTATATTATAAATGTTTAAATCTGAAATAACAAAAAAACAGCACTTTTAGACCAAAAAATGGCTTAAAAATGATGTTTTTTGTTAATTATTAGTTATTTTTTAGATTATTTTTAATAATTAAAGATCATTTTCTAGGAAATCCTGAGGCTGTTTTTATTAATTTTTGGTGAAAAATTAAAACATTTTCAATATTGAAATATATTGGCAAAATTCTTGTTTTTAAATTAATATTTAATTTGTTAACTATAAAGTTTGAATTAGCTATTTCTTGCTGATATTTATTTGATTTTAAAAAGCAAGCAATTCCATTAATTTTTAAAAGATGGTGTGAAATTTCTAATAAATTTTTTAATTCACTCACCGCTCTTGCACTAATAAAATCAAAACATTCTATTTGATTTGAATCTTCAGCACGAATTTTTTGTATATCAATATTTTTCAATTTCAACTCATTTTTAACCAAATTTAAAAAATTTACTCTTTTTGATTGAGGTTCATAAATTGTTAAAGAAAATCTTGTTTATATATGAAATATGGAAGGATAGGAAATCCTGCGCCCGAGCCAATGTCGAGTACAGATTTGTTAGTTAGATTACAAATATTTTTTTCAATGTCTAAAAAAATTAAAATTGATTCAATTATTGCTTCTTTTAGAAGCTTTTCGTTATAGAAACCTGTTAAATTATATTTGCTATTTTCTTGTTCTATTAATTGATAATATTCATATAATTTTTTTAAGACTTCTTTGCTACATTGCGGTAGACTTTGCAATATTAAATTATTGCTTTCTTTTTCCGTAAGCATTATATACTTCTGTTATAGATTTGCTTTCAATGTTTGCATTGATTACCATACTTAAAAATTGAGCCAAAGATAATTCTCTTAATTTTTTAAAGTTTGATAAGTGGCTGTGATCTATTGAATTAGTAATAATAACTTCATCAATCATTGCGTTATTTTCAAACATTTCAAACCCTTTTGAAAATAAACCATGAGTAGCTGCAATTATAATATTTTTTGCGCCGTTTGCTTTTAACGCTTCTGCTGCATGAATAATTGTTCCACCTGTGTCAATAATATCATCATAAATTAAAACATTTTTATCTTTAACATCACCTAAAACACCCATAATTTCGCATTTATTAGGTCCTGTACGACGCTTGTCAATAATTGCAATTTGTTCTTCATTGTTTAGTAATTCTGACAATTGTCTTGCTCTAACTGCACCCCCGTGGTCTGGTGATACAATTACTAGTTTTTCTTTTCTGTTTAGCAATTCATTTGAAAAAATAAATTGTCCCCTTAAATCATCAACAGGAATGTTGAAAAATCCTTGAATTGAAGCGTTGTGCAAGTCAATAGTAATTATTTTATCAGCACCCGCTGTTTCATACATCTTAGCAACAAGTGCAGCTGATATTGGATCTCTTCCACTTGCTTTTCTATCTTGTCTTGCATATCCATAGTAGGTCATAACGCAATTAATGCTCTTTGCTGATGAACGTCTTAATGAATCAATTAAAATCAGCAATGACATTATGTTATCGTTTACTGGCTTTGAAGTATTGCAAATAATGTAAACATCCTTATTTCTAACGGTTGTTTCTGATTTTAAAAGGCATTCTCCATCTGCAAATACTGTAGTTTTAACTGGCAATATTTCTTTATTTAAATACTTTCCAATTTCTTGTGCTAATTTTTGGGAATTTTCCATGCCAAAAATTACAACATTATCTTCTTTTTTTGCTTTCATTTAAATCCTTATATATATTCTCTTTAATTATTTATAGTTAAATTTTACATTATTTTATTTAAAAATAAAATATTAATAAATTGCAATTAAAAAAGTGCAAGTCGCACTTTTATAAAAATTCTTTATATATACGTTTATAAATTGACTTAACACTAACAACTAATAAGACATATATTCCTAAAAATAAAACTAATCAAGCATAATAAATTCCTGGTAATGGCATTAGACTTAAACCTTTATTAATTCCCGGTATATATGGCACAGCATTAATTATTGCACATCCGATTAATGTTGAAATAAATAACGAGGTCGTTGCATTTGATTTAATAAATGGTGTTTTATCAGTACGCAAAAAGTGAATTACAAGGGTTTGAGTATACATAGAAACAACTAATCATCCTGCTCAGAATAGTTCTTTAAATGCTTTTTGTTTTTCTATACTTAGTTCATCTTTTAAAGAAGCAAAACTCTTATATGCTCCTATTTGTACTCAATCTCCCTTGCTAGTCATAAATGACGCATTTGGAATTATTGCAAAGAATAAGACTACATATGAAACAATATCTACTATTGAACTCGTTGGACCAAACCAAAACATAAATCTTCAAATTGATTTATACTCTCATTGCCGAGGTTTAAGAATAAATTCCGGATCTACATTGTCTCATGGCATTGTTGCACAGCAAACATCATATACCAAATTCAATAATATTATTTGTACAGGGTTTAATGGAACAAAAGGAATTAAAAATGAAGCAATTAAAATACTAAATATATTTCCAAAGTTTGAAGATGCAGTCATTTTTATGTATTTGTTAATATTGGCATGTGTTTTTCTACCTTCAACAATTCCTTTAGCCAAAACATTTAGGTCTTTTTCTAAAAGAATTATGTTAGCTGATTCTTTTGCAATATCAACTGCAGTATCAACTGATATTGAAACATCGGCTGATTTCATAGCGGGTGCATCATTAATTCCGTCACCCATATAGCCAACAACATGACCATTATCACGCAATATTGAAATAATTCTTGCCTTTTGGTCAGGGCTTAATTTTGCAAAAATTTGAACTTTTTCAACTTCAATTTTAAGTTCAGCATCACTTAATTCTTCAACTTCTTTGCCTAGTAAAATTCTATTATTTTTTATTCCTACTTGGGCACATACTGCTTTTGTTACAAGCGCACTATCTCCCGTTAATATTTTTACATCAACTCCATATTCATGCAATTTTTCAATTGCGGCAGCAGCAGAATCTTTAGGAGGATCAAGAAATGTTAAATATCCAATCAAAGTCATATCGCATTCATCATTTACTGATATTTTTCCAACTGCATTAATTGTTGATTTTTTAGAAGCTATCGCAATAACTCTTAGTCCTTCTGAATTGAATTCTTCAACTCTTCTTAGAACCTTATCTATTGTTTTTTTATCAAGAGGCTTAACTTCTCCATCAATGTAAATATTTTTGCATACACTCACAATTTCTTCAACAGCACCTTTTGTAATCAAGGTTACTTTATTTTGTTTTAATGATTTAACTAAAACAGACATTCTTTTTCTTTTGAAATCAAAAGGAATTTCATCAATTTTTTCATAAAATCTTTGAAAATCTTCAAGTTCTTTGTTTTTTGATGCCATTTCAAAAGTCTTATTAATTATTGATTTATCAAGTAAATTTTTCAATCCTGTTTGATAATATGAATTAATAAAAGCATATTTTAAAACATGAAAATCTTCTTTGCCGGTAACATCCATATGACGTTCCATAATTACTTCATCTTGTGTTAATGTTCCGGTTTTATCTGTACATAAAATGTCCATTGAACCAAAATTTTGAATTGAATTAATATTTTTAATTATTGTTTTGGCCTTGCCTAGTGAAATTGCGCCTTTAGATAAACTAGCAGTCACTATCATTGGAAGCATTTCTGGTGTTAAACCAACAGCAATTGATATACCAAACAAAAATCCTTCTAATCATTGGTTTTCTCCACCAATAACACCTTTAATTCCTAAAATTAAAAATACTATAGGGACAATAACGGCCATTATAATAGTGAACATTATTGAAATTTTTTTAATTCCTTTATCAAAGTTTGTTGGTATTGGTTTTTCATTGATTTTGGAAGCAACTTGTCCAATAATTGTATCATTGCCGGTTGCAATTACAATAGTTTTAGCAGAACCTGAAATAACATTGGTTCCCATAAAAGCTAAATTGCTACGATCAGTTATTGCTTCAAATTCTTTAGAACTGTCGATGTTCTTATATTTTTCAATAGCATCGCTTTCTCCGGTTAATGAGGATTGAGAAATAAATAAGTCTTTTGCTTCTAATATTCTTACATCCGCCGGAATAATATCTCCAGCTGCTAAATACACAATATCGCCTACTACAATCTCATCCATTGGAATTTCCTTCATAATGCCATTCCTTTGAACTAGCGAAGTAGTTTCAACAGTCTCAACTAATTTATCAGCGCTTGATGCCCCACGTTGTTCTTGAACGAAGTGAATAATTCCACTTAAAAAAACCATAAATAAAACAATGATGACAGTCATTCCATCTATTCATGTTATCTTTTTACCTTTTGATGCCGGCAATATTAAATCAATAATCATAGAAACAATTGCCAATGCAAACAAAATGATGTTAAAAAAATTAAAAAATGATCTAAATAAACGCTTTCAAACATTATCAGCGCCTTTTTTTGTTATTTTATTTAAACCATATTCTTCTCTTGCAACTTCAATTCTATCTTCGTCTTGAAATCCATTTTGAGAAGTTTGATATTTTAAAAATAATTCTTCTTGACTACAATTTGAAGCTTCAACTAACCTTGTATTGATTTGCTTAATTTGTTTTTCTTTTCTTGAAGAATTTTTAAGTTTATTTTTTGTTATATTAACTATCATGTTATCTCCTTTTTATTTGGGTATTTATTATAGGAGAGCGAAATATAATTAAATACTAAAAGTTACTATGTAATTTTTAGTTAAATTGCTATATCGCTCACAACTTGGTAATTCCATAGTACCTCCATATACTTAAGTTTATAAAAGTATTTTAACATATTTCAAAATTAATTTCATCTAAACTTAAAAATTAAAAATTATATAAATCTTTTATTTTTAATTCATTTAAATTTTTTGTTTCGCCAATTTCAATAATTTGTCCATCTTTGACATAAATGTATTTCTTAAAATAAGTTTTTATTAATGAAATGTCGTGAATATTTACTAAAATTGTTTTTTTGTATTCTTGTGATATTGAAGACAATAATTCTAAAACATCTCTTGCGTTTGCTATATCAAGACTATTAGTAGGTTCGTCTGCTAAAATGATGTCAACTTTTTGTAATAAAATAAGTATCAACCACAATCTTTGCCTTTGACCCCCGCTTAATTCATCGACTCTTGTAAATAACTTATCAAATATATTGAATTTTACAAAATATTCATATATTTCTTGTTTTTGGGTTTTATTTAAAACATGAAATCAAGAAAAAAGAAAATTTTTATATTTTGGATAAAAATGTAGAATGTTTTCATAGCAATTTAAACTTTCAATAAAATTATTGTTTTGACTTAAAAATCCAATATTTTTTAATATTGCCTTTCATTGTTTTTTTGAAGCACTATAAATATCAATATCATTAATTTTTACATTGCCTTTTTTTACAAATAATTGCCTCAACAAAGCATTGAAAATTGTTGATTTTCCAGCTCCGGATTTTCCTATTATCGCTATAAATTCACCCTCTTCAATAGAAAAATTTATGTTCTTTAAAACTATTGGACTTTTTTTATAATAACTTGCATCAACATTGATAAATTCAATTTTTTTCATATTTAAATTATATTTGGAATGTATCGATACAAAACAAAATATTGCAAAAATAAAAACATTAAGCGTGTTCTGCTCAATGTTTTTCAGCGAGGCTTGTATCAACCTCATTAATTTTTATTTAATAGTAATTTCAACATCCAAATTTTTTAATACTTGAATTGCTGTTTCTTTAATTTCTTTTGAATATTCATCAAATTGTTGTTGTTCATCTTCGTCAAGTCTAATTTTTGGCAAGTGTGTATAACCATTCTTACCCAAGATTACTGGCAAACCAAAGTAAACTCCGGCATGTTTATATTCCTTTGGCAATTGAACTCCAAGAGAATAAATTCTTTGTCTATCATACAAAATTGCTTGTGTAATTTCAGCAAGGTTTTCGCCAATACCATAATTGGTAATGCCTTTACCGGCAATAATTTCAAATGCTTCTTTTTTAACCATTTCGTTTAATTGAGAAAGTCATTCATTTGTTATAGTACCATTAGCTAATCAATCTTTTAATGAACCCAAACCAATTCTTATTTTTGAAAATGGGATTAAGCATCTTGCTCCATGTTCGCCAATAACAAATCCAGTAATTGAGTGTGCTTTAATTCCAATTTTTTCTGAAAGAAATTTTTTCATTCTGGCAGTTTCTAGAATAGTTCCTGAACTAATAACTTTATTTGGAGAAAACTTGGTTACTTGTTGATAAATTGTTGCCATAATGTCAACAGGGTTTGAAGCAATGATTGTAACACCAGCAAATCCTGAATCTTTTATGCTTTGACCGATTTTTCTCAATAGTTTTGCATTATCGGTCAATAATTCCATTCTATCTTTTAAAACGCCTTCCTTTGGTCTAATTGATGCAGTAATAATGACAATATCAGCATCTTTCAAATCTGGATAATCTCCAGCCTTGAATGTTGAACCATTATTTGCCATTGAACAAGCAGCATCATTTAAATCCTTTGCTTGTGCAATTGCAACTTGAGGAAAGCTATCAATTAAAACATAATCTGCTTCTATATTTTTATTTAATGCAGCATATAAATATGAACTACCAACAGCACCAACGCCAATCAATCCTATTTTAGTCATTTTTTTTGCCCCCTAATAACTCTTTAAAATTTTTTGCCAAATTAAAATTGTAAGATTCCAAAGGCTTTCTTTCAAATGGATTCTGTTCATTTAAATAGGCAAATAAAATACTTGAATGATGTAAGAAAGACATTATTCAACCCAATGTACTATCATTATAATTGTCAATCATGATTTGAATGTTAGGTATTTTATATACGTTAACATGGTTTTCAATAAATGTTTCCATTACAAGATTACATAAATTATTATAAGTTAAATTGCTTAAATAATTCAAACCATCTGTTTCATTTTCATTATTTTGTGAAATTTTGCAGTCCATAATAGGTTCTTGAAATTTCAAACAAGTATCAAAACACTTAAATGGAGTATTGGTGTAATTTGAAGCCAAAGTTTTATTATCAACTCCTCCAAAGCATGTTTCAATATAAATCCTGTTATTTATAGATGAAATAGTTTCACTAAAAAGCATTTGTCACAATTTTGCTAAAGATTCTTGACTTTGCTCGAAATAGCAAATATTTTCAAATATAAAATTGTTATCATGAAGGATTTTTCTAGTGATTGCATATAAAACTGCTGGGTTTTTTTCTAATGGTGCATTTAAAAAAAATTCATTAGCCTTATGAGCACCAGATAAATATTTTTCAATATTAATTCCTATACAAGCAATTGGTAATAAAATTGCAGGTGAATAACTTAAAAATCTTTCGACGGTATTATCTAAAATTGTTAAATGAGTATATTGATTTATCTTGGCATCATTAAATAATCTTCCATAATTATTGTTTGTTGTTACATAAATATATTTTGAAGCACCTGCCTTGCCTAGCTCTTTATATAATAAGTTTTTTAATTCTCTAAACATTATTAAGGTTTCTAAGCATTCGCCAGTTTGTGAGATTACATTAATAGCAAACTTTTTATTTTGAAGTTTAATAAGCAATTTAGCAACTTCTAAACTATTAAAATTTTCATTAACATAAATCAATTTAATTGATTCATTTTCTTTTCTTAAATCAAAATGCAAATCAGCAAGTGCCTTAATTTGATTAATTATTGTTTGAGTCGCTATTATTACTAAATATTCAATACCATCTGATAATAATTCGCTTGATATTTCTTGAATTTTTTCATAATCAGATTTCTTTACATTTTTAAAAACATTGAATACACCTAAATAATTTGAATTTTTAACCTTTGAGTTTTTTATATCTAACAAGATTTTTTGAACCGCCTCATAATAACGGTTTGGTGCACTTTCTTTAATTGCTTTTTTTAAATTTATGTCAATAATTTGCTTATTTTCCATAATTAATTCCTTTGTTAGTAAATTCTAATAATCCATCAAATTCTGCATCTTGACTATCTAGTTTAAATTCGAAAGGGTTTCTTAATTCCTTGGGGTGAATTCTTTTAAATGAAACTACTAAATCATTATTTGGCATTATTTCAATTACTTGAACTTCCTTAATATCATTGATTTTAAACATTAATTTTAAATCGCTAATGTAATAGTCACTAACTTCATTTATACTCAAAAAGCATTTACATCCTTCTTTTGTTTGCAAAATAATATTATTCTTTCAAACCTTTATTATTTTGGCTTTAAAAATATCTCCTATTTCATAATTCATTTTTGCCTCTATTTTTATATATATTTATAATAAAATTCCTAAATTATATTTTTGTATAAGTTATTAATTAATTTAATAATCATGAATATATAAATTTAAAATTTAATATATATTAATTATTAATGCTTAAATTATATAATATATAAAATTTAAGTTTATACATTTAAAATAAAATGTAAAAATTAATATAATTAGTTTATGAAAAATAGTTTTTATAAATGCTTAAACATTGCAACATTGACAGTTTCCTTGCTTTCAACTAGTCCTATTTTGACGATTAGCTGTGATGTAAAGAAAGATGTTGATTTATTGCATTTTAAAATGACTGTTAATAAGCCTTGAAATGGTTCAAAAAATGATGAATACTTTAATCAACTAATTAAAGATATTAAACAAAATAATAAGAATTTAAAATTTGATTTTAACGTTTCATATATTTTAGACAATGTCGATATTGCCTCTGCTATTAAAGCTAAAAAAGCAAATATGGCTTTTATAACGTCAACTTTATTAAAAAATTCATTGCTAAAAAATGATGTTATTCCTTTACTTCAAACAAAAACAAGGGCTTTTTATTTTGATAAATTAGATGAAAAATATGTTGATGGTTCTGGCAATGATCCGCTAAGAAAAATAGCAAAAATAGCAAATGATTTATTTAACAAAAAGCCTTATAAATCATGAACAGATAACGAATATCAATGAAATGGAAAAATTTATGAAAAATTTTATGATAAAGAAGGCGTTAATGTAAATTATCAACGAGGGTCAATCATGATTTTTGGAACCGATTCAGAAATTAAATCAATTAAAGAAGCATGAGAAAATAAAGACTGAAATGCATTTAGAAATTTTGGAATTGTAATCGGAAAAAAATCAAGTGGAACAAAATACATAGCACAAGAGGATTTATTTAAAAAACATTTCACTTTAGCAAATAATAAATTTTCTTCTTTTGCAAATGATCAAATAAATAATGCTAATAAATACATTACTGAAGAACCTAGAAACATAGGTAAGGGTGCACTGTCAAAATATCACATTGTTTTTGATGAATTTGCATCCTTTGCATATACACACAATCGAAAAAAATCAAAAAGCATAAACTATGATTTTTATTGCCCTGATGGGAATAAAAAGATTGAATTACTAACAGTAACAGATCCAATAAAATTTAATGTCTTAGTTGTTGATAAATCAATACCAAAATCTGCTTATCTTGCTTTTGCAAATTCAATAATTAATTTAGCAAAAGAAAAAAAAGATACATACGGACCAAATGTTGGTTTCAATGAATATGAACTAATTAATGAATCTGACTATTCAAAATATTTTTAATTATTAGCAATTAAATTAAGTATATAATTTTTTTATAAAGAACAGGGGTGCCTTTGGCTGAGAAATACCCTCATTAGCTGATCTAGTTAATACTAGCGTAGTGAGTGTCTTAGTTTTTTGCATCCTCTTTTAGGAGGATTTTTTTATGCTAAAATTTAAAAAATATTTGCTTATAGGGGCAGCGGTATTGCCTGCAATGGTTGGCGCATCAGTTTTATCTTCATCATGCGTTGATAAAATTTCAAACAATACTAAAACTTACGAAAATTTAAAATTTTCAATTAACAAGCCTTGAAGTGAAACAAGCAATAAAGATAATTTCTTTTATGAAGTTATTAATAAAATTAATGAAAATAAAAAAGACTATAAAGTCAACAACGCAACGTTACATTATTTAGACGATAATGTTGATTTTATAAAAGAATTACAAAAAGGAACTGCTGATATTGCATGTTTAACACCAACGGTGTACAATCTAGAAAAAGATAAATCAAATATTCATCCATTTTTGCAAACAATGACAAGAGCGTTTGTTTTTGATCAAGATTTTAACGCTCAATATTCAAATGGTCTAGATGATGATCCACTAATAAAAATAGCTAAATCAGCATTTGATTTATTTAACAAAAAACCTTTTGAAAATTGAAATGATAAAGAATATAAATGAAATGGTTCAATCTATGAATCATTCTATGCAAATAAAGACAAATTGGTTGATTATTATAGAGGGTTGGTTGCTATTTGAGGAACACAAGAAGAACTTAAAGCAATAAAAGAAGCCTGAAATAAAAAAGATTGAAAAACATTTAGAAATTTTGGAATAATGCACGGAAGTAGTTCTAGTGGTTCGAAATACTTGCTTCAAGAAGCATTATTTAAAAAACATTTTAATTTAAAAGGAAATGAATTTAAATCATTTTTAGAAGATAAACAAGGAAACGAAGCAAAATATATTAAAGGCAAGATTAAAAATATTGGAAAGGGTTCGTTTTCTAAATTCCATATTGTTTTTGATGACCTTGGATCTTTTGCATATACTCACAGCAAAGGTAGCACAATCTATAAATTAGATGACAAAAATTCAAAAATTGAATTTTTAACAGTTACTGAACCTTTAAAATATAACATTTTTGCTACATCATCAAATATTCCAAAAGAATTAAGAGAAATAATTGCAAATGCAATGGTTGAAGTTTGAAGAGAAGGCAAAGATACATATGGACCTACTGTTGGATTCAATGGTTATAAGATAATAAATGATCCAATCAAGGAAGTTGTATTGCCTTATAAAAATGTATTTAATATAAATTAGTTATGAATAATATTTCTTTTCCAAAATCTTTCTATTTTGAAAAAATAAAATACGATAATTTAACGTTAAGGGGACCAAAAAAAATTATTTGAAGAATTTTAGGTCTCGTTTTTGTTTTAGCCATAATTGCTTTGTCAATTTATTTTATTGACTTCTCTTTTGCACCAAATGGAAGAAGCGTTTTTAGTCAAAATATAATTGATTTTTTTAGACCACATTTGATTAGCAAATTAATTCCTGGTCAAAATTTGTTTTTATTATCAATTAAATATTTATGAGAAAGCATTAAGGTTGCTTTTTTAGGAACAATTATTGGGACATGTTTAGCATTATTTACAGCATTTTTTTCTAATTATAAAATGAATAAATATTGAATAGCAATTCCAATAAAATTCATAATAATAGTTTTAAGATTACTACCAGAATTATTCTTTATTTATTTATTTCAAATTTCAGCTGATAAAACATTGGCTATAAATTTGATTTTCTCTTGATTTACTTGGCTTTGATTACACGAATATTTTTCTCAAGTAATTGAAAATGCAAATTTTGAAATATTCTATTGGCTTACAAAAATAAAACAAAATAAACTTACTGCATTCTTTAAAGAAATTTGACCTCAAATATCTCCAAAAATTCTTAATTATATATTTTATGCTTTTGAATCAAACATTCGTTGATCTACAATTCTTTCAAAACTTGGTTTTTTAGGAATTGGAATATTGATCTATCCAAATTCAATTAATGTCAATTACTTTCATGAACTTCTAATTCCACTACTAATATTGGCAACATTTTTAATTTTTATTGAATTTTGTAACTTTATAATATCAAATTTATTGTTTTATTCAAGAACTCAAAAAGTAAATATAAAAAAATATGCCAAAAATTCTACTATTAAAAAAATAATAATTTTTATAGCAATTGCAATAGGATTAATAATAACTATCTTTGCATGTTTATCATTAAGGGGAGAAAAATTTTATTCAACCCAAGGAAATTACTATTTCAAAGAATTATTTAATGGTGAATGAAAAAATGTTTTTCTTGGATGAAAAACAAACAGTAATTTTGGACTTCTATATATGCTTTTGCAACTTGGGGCATTGGTATTTTTAAGCATTTTATTAACATATTTTATATCGTATTTTAAACTGCTATTGCTTTCAAGACAATTATTCAAACGCTCATATTGAATTGAAAAAATTTGAAACACTATTTTAAGATCAATTCCTGTAATTTCATTATTTTTATTAATCTCTAATTTGTTCAATAAATTAGAAGCTGCTTTTGTTATAGCATTTGCAATTCATAGTTCTTCTACTATTTCTAGAATGTTGGAGAGCTCCATTAAAAAAATCTCGACAAACAAGATAAATGAACTAAAAATCAAAGGATATTCAAAATTTTATATCTTTAGAAATTTCATAATGCCAACAATTAAGCTAGATTTAATTACATTTATGACTTTTGAAATTGAAAAAATAACTAGAAACTTTATTACATACGGTTTATTTTCTGCTTCATTATTAGGATCTGAAACTAATCTAGGAAGATATAAAGAAATCTCCGATATTGCACCATATTTATGAATTGAATTTATAATTATTGGATTAATTAATCTAGGTAATTATTTATATAGATTAAGATTAAATAAAGGTAGTATTAAAAAATAAAATAATATTATTTAAATATCACTAGATAAATTTATCTCTAGTGATATTTTATTAATTACAAACTTTTAGATTTAATTAATTAACTTAGATTTTTAAAAGAAATGTATTATTTAAAAATTATGTAATTTATATAATGTTTGTTATAATAAATATTAGACCTAGGGGTTTAGTATAACGGCAGTACCGCAGTCTCCAAAACTGCTTGTGAGGGTTCGATTCCTTTAACCCCTGCCATATAAAAAAAGTTTGTTAATAGCAAACTTTTTTTAAATTCAATATTTAAAAAATTTGCTTTGTTAGCAAATTTTTAATCTAATTTAATTTTGATATTTTTTAGCAACATATTCTTTAATTTGATCAACGCTATCAAGCTTTTCTCAACTTAAATTAAGGTCATTTCGACCAAAATGTCCGTATGTTGCAGTTTGTAGATATATTGGCTTTAATAAATCTAATTTTTTTATAATTTGTTCAACGCTTAAATCAAATACATGTTCAATAATATCAGCAATTTGTTCATCATTATATTGTGATGAATTAAATGAATTTATATAAATAGATTGAGGACGTGGCAAGCCTATTGCATAAGACAATTGAATTTCAATCTTATCTGCTATCTTAGCAGCAACTAAGTTTTTAGCAATATACCTTGCCATATAAGCAGCAGACCTATCTATTTTTGTAGCATCTTTCCCTGAAAATGCTCCTCCGCCATGGTGGGCAAAACCACCATATGTATCAACAATTATTTTTCTACCTGTAAGTCCAGTATCTCCAACAGGGCCACCAATTACAAATCTGCCAGTTGGATTAATCAATAAATCATAATCTAAATTGAAATTAAATTCTTTTAACACATAATCAACAATATTTTTCTTTATAAAATCCTTAAATTCATTTAAATTTGCATTTTCAGCATGTTGAATTGACATTAAAACTTTTTTAACAGTTATTTTATTGCTTTCATCATCATATTCCAATGTCACTTGGCTTTTCATGTCACTTTTTGCATATGGAAATTGATTTTGTCTTCTTAAAAATTCAGCGCGTTTAAGAAGCTCATGTGCTAAAACTATTGGTCAAGGCATATAAAATTCATTTTCATTAGTTGCATATCCAAAAATAATCCCTTGATCACCAGCACCCAATTCATGGCCTTCTTCTTTATTGACACCCAAAGCAATATCGGCACTTTGTGGAGCAATACCATTAATTATATTAAAATCATTTTCATCATATCCTAATGGTTTTAATACCTTTCATGCTTCTTTAATTGTGTCAGAATATGCCTTTGTAGTTATTTGACCGCCTATATAAATGATGTTGTTGTTTGCCAAAACATCACAAGCAACTCTTGCATATGGATCATTTGTCAACAAACTATCTAATATCGCATCACTAATTTGGTCACATATTTTATCAGGGTGACCTGCCCCTACACTTTCTGATGTTAATAATTTTTTCATTTAAAAAACCTTTCTAACACAGAGAAAGGTTTTTATTATCTTTTTTTGTACATGTTAGATATAAATCCCTTACCATCCACCTTGCTTTATTTAAGTAGGTTGGCATGCCTTAATATGGTTAACTGGCAACTCTCTATGTATTTTTTGTTTTATTATTATACTAAAAGTTTAGTATATTATTAAAAATTAATAATTATAAAATTATAATGTTTTCATTTGCTGATTCATCTAAGGTTTTTTGATCTCATGCAGATGCTTGAACTTCACCAATATGATGTTTTTCTAATAGAAACATTGCAATTCTACTTTGGCCTAGACCACCACCAATCGTATATGGCAATTTGCCTTCTATTAAATCACAATGATATGGACTCAATTTTTTAATTTCTTCTTCGGTTTTATTTGATTGTTTAATTAAAGAATCTTTATCAACTCTAATTCCCATACTGCTAATTTCCAATGCTTTTTTATTTACTGAATCATAAACAATTAAATCCCCATTCAACATTCAATCATCATAGTCGAATGCTCTTTTTGAATGTGGAATTCCATCAGGTAGATTGTATCCTATTTGATAAATAAAAACAGAACCATATTCTTTGCAAATTACCTCTTCGCGTTCTTCAGGACTTAGGTTTGGATAAAGATTATACAAATGGCTAGAACTAATAAAATAAATATTTTTAGCTAACTTTTGACTCAAAACAGGATAGATTCTATTAATAGATTTTTCGGTATTCTTTAATGCTCCATAAATTAACTTAACAATATGCTTTAAATATTTTAAATTTCTATCTTCTTTTTTTATAATTAACTCTCAATCTCATTGGTCTACATATATTGAATGTTTATAATCTAATTTTTCTTGTTTTCTTATAGCATTCATATCTGTATATATTCCTTCATACAATCCAAATTCATATTTCTTTAAAGCATTTCTTTTTCATTTGGCAATTGAATGAATAATTTGCAATTCATCTTCTACTTCGTCTGCAAAAAATACAACTGGTTTTTCGCCATTTAAACCGTCATTTAAACCACTCTTGGTTTTTACAAATAATGGCGCTGAAACCCTGACCAAATTCAATGTTCTATGAAGTTGATGTGAAAACAAATCTTTTAGTTTTTCAATAGCAAATTGGGTATCTTTCAATGATAATTTTGATGATGGCATATTTTATGCTCCTTTTTTAATTAATTTAATAAATTATAAATTAATATAACTCTTAAATATAAATAATTTTTTAATACAATAAAAATCAAGCCTAGTGGCCTGATTTTTATTACTTATATTGCCAAATTTGAAACTATTTCTTTTCAACAGTTAGGCCGTATTTAGCACTTACTGATTTAATTAAAGCATCCATTGGCAATGCACTATTACGTAATAAAGCATCAAAGAATTCTTTTACATATTTATGATTATCTTTTTCAACAAATTCTCTTCTTGATAGTCCTAGTTTCTTTTGAACTTTTGTATATAGATCCATCATAACTGCCTTACCTGAGTTATATGAGGTTGCTTGACCAACATATGATAGGTAACGTCTTGATTCTGATGTTATGTCACCAATTCCTAGTGATGAATTCTTCTTCATAAATTCTCTTACTTGGTTAATTGAAGCACCTGCTGGCAAGTCTTCATGTCCTTTTACACCTTTACCATGGTAAGCAGTGTCTAATGCTAAACGCATGTTACGCAATTGAGCTTCGTTTAATGCACCTTGGTATTGCAACAAGTTAGCCAATTTTACAGCATCCATTGCATGTTTTTTATCTTGGTTTTCATATTTATTAACTTGTGCAACTTTATTTCAATATACACCATTGTGTAGGTCTTTAATTTGTTTAATCATGTCATCTGTAACTTCTGCTTCTGTTTTAGCAGTAAAGAAGTTTGTTATACCATGTGCTGTTGAGAAGTCAACTGGCATTGCATATAAATCGCCATCTTTATTGTCATAATCTGGTGTGCCATAGAATCCAGCTTCAATTCCAAATCATTCCATAAATAGAGCTCAACCTTCAACATATGAAGTGTAGTGGAATACATTTCCTAATTTATATTTACCAAATTCTCCAACTGATGATAGATACTTTTGAGCATACATTAATTGGTTGTGGTGTCCCATCATTGATTCATGGTTTGCAAATGAAGTAACTGATCATTTTGGTAATGAGAAGTATGGGTCAACATTAAACATGAATTTTTTAATGCTTGAGTCGTAAGCTCCAACACCTGAAAATTCTCTTCTAGAAAAGGCATATGTTTGAATATCATAATCAGGAACGGGATTTGCAAAGAATTTACGTCCATAATTTTGAGTAGTCTTCTTAAATTGATCATATGCTTTAAATGCTGACAATCCACCATAATAGAATTCTTCGTTTGTTATCGAACCATGTTTTGTAGCTTTATTTTTTTCACTTAGTAATTGATTATAATTGAATTTTGTTAATTCATCAACATATTTCTTCAAATCAGGATTTGTTTTAAGACCACTTTTTACAGTATCTGTTCAGTAGGCACTACCTTCACGTCCAAAGAAGAATTCTTCATCATTTAATCATTTAGTAAATGAAGGAATATCAATGTTTCCTTTTTCATCACGAATTACAACAGTTTCTTCTTTTATTTCTTCTGGTCCAATACCATCAGCATCATATTTAATTTTGGCTTCTCATTTTTTGCCTTTTCCAACAATTAAATCAGCTGCTTGGTTAGCAATTTTCTTTGAGTTTTCAGCAGATTTCTTTGTTGTTTCATAACCAATATTATTTACTTGATCATCTGTTAAATCAGAAGTTGTAGCCATCTTTAGAATTTGACGATACATTGATTGACCATTTACGGTAGCATCTCCTTCAGCAAAACCCAAACCAACATTTCTTTGATTTAGGTCTTTTTCAGTTAATCCTAAACCATATAATTTAACAATTTCCCCACTAGCATTTTTTGCATCAATAGACAATTCTTTTTCATTAACTTTATTTTCTTTATAAAGTTTTAATTCTTTAATGTCTTCACCCAATCCATGAGTTGCAGCATTGTAATATGTAGTTGCATAATATTTTAATAATTCTTTTTTGGCTGTTGGCAAGGTAGAAGAATTAATTAATTCTACAACTGAAATTTCGTTTTTAGACTTATCATTTACAAAATTAGTTAGTTGTGTTTGAAATAAACTTCCTACAAAAGCATTAATTACCATTTTTGAGAAAACTTTCGACATAACAACTTTATGTTGCACTGCTTCTTTGAAGTTTTCTAATAATTGAGGTAAACTGCCTCATCCTCTTCCTAGTTGATTACTAAATGCATTCATTGGATATACAGATGCATTTCCTCAAGAAAATGTAGGGTTTAACAATCTTAGTTGATTGTCATGGTGTCCTTTAGCAATTTCTCAATCGTATTTTATAGCATATTTTCATGCTAAAGAATCGCTCGTTAATGAGGCATCATTTATTTTTGAAATTTTTTCAATATATTTACCATAAATTTCAGAAATTTTCTTTGATTGTTCAGCAGTAGGTGCACTTATTCCATTTAATTTTTCTTTCTTTAATTCAGATTCAAGATCACCTTTTAAATCTAAAATATGTCTTGCGGCTGCTTCTTGGCTGGCTGGTGAATTATCATTTTCTTTTGAAAGTAAATTGCTTAATTCATCGCTCTTTTTAACAATATCGTCATATTCTTTGTCTAGTGCATCTAATTCTTTTTGATTAGCTTCAATTTCTTTAGAAATATTTTCTTTTTTTGTTGTCAAATTTGCAACTTCAATTTCAAGTTTTGACTTTTTATTAGTTTCTTCAATGATTTTATCTTTTTGAGTTTTTAATTCTGTTTGAAGACCTTCAAACTTTTCTTCTTCTTGTTTAATTTCTAAATTCTTAGCTTTAATTTCTTCCTTTTTTGATGATATTTCATTATTTTTTGTGCCATCAGAATTTTGTTTTTCTAATGTTTCTAGGTTTTTATTTAAATCATCCAAGGCTTTTCTTAATTCAACTAATTTAGTTTTAGATTCTTTAATGTTATTTTCTATTTCTGTTGATTTTTTCAAATAACTTTCTATTTTTTTCCCTGAATTTTTAAATTTGCTTTCAGAAGACAATAGCTTGGTCTCTAAGCTACTTAATTGATCTTTAAGATCATCATTTTTTTCATTTAATTCTTCATATTTCTTAGGAATTTCAACAATAGCTTTGTTTGCAGCATTTAATTCTGCTTGATATTTAGCTGTTCTTTCTTGTTGGATTTTATTTAAATCTTCTTGCAATTTTTTAACTAATTTTTCTTTTTCTTCAATTGTTTTTGAATTATTTTTTGTTTCTTGCAATTGCTTCATAAGATCATTAATTTGTGCTTGATCTTTTTGAGAATTTGCTTTTGCTTCATTTAATTGCTTTTCTAAAGATTCAATTTTTTTGTTTAATTCATCATTATTTGTATGTGTACATGATGCTGCCATAGGCATTAAGGCTGCTACGCCAAAAATTGAACCTAATGAAATTAGAATTCTAGTTTGTTTTTTCATTTTTCCCTTCTTTAACTTTTAACTTATATTGTTCTTTTTTACAACAAAGTTTTTATCTTTATATAGTCATAATTTTAGAAAAAAAAAAAAAAAACGCTTATTTTGTAATTTTTTTGCATATAAAATACAAAAAAAATTAACATTTTCTTATAGTTTTAAGTACAAAAAAGCATAACAAAAGCACAATAAAAATTTTTTTGCTAACTTTAAATATTAGAATTTATGCTTTATTTTTGTAAACTATATATTTTTTAAAAAAGTGGATGAAAATCAAAAAATGATGATGCTTTTTTTAAAAAATCTAACAAAATAAAAAATTTTTAAAAATTTTGAAACATATTTAAAAATAGTATATAATAATACACGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCTTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTTTTTATATCTTTTTTTAATAAAAAATAAGGCTTTTTCAAACCTTATTTATATATAGATTAATATAAGTTGTGATTAACAATTATTATTTTTGAAAATTTTTTTACCTAATTTAATATACTTATCAACTTTTTGATTTAAAACCTCAAATGCTAAATTATAAATATCTTCATTATAAATATCAACACCAGCATCTTTTAAAATGTTTGCAGGTCAATCGATGTCTCCGGCACTTAAAAATTTATTTATATAGTTTTTTAATGCTTCTTGGCCTTCTTGTTTGTATTTTTGGAAAAATACATTTGCTACGATATATCCTAATGCATATTTATATACATAGAAATAATAATAGAAATGAGGAACCATTACGCTATAAACATTTCTAGGATCGCCTAGTTTTATTGATTTTGATGTAGTCGAGTATTTCTTTTCGTTTTCAACATATAATTTTTCTATTTCTTCATATGTATTTAGTGGTTTATTTTCATCTATTGCATTATATAGATCAAATTCAAAATTAGATCACATCGTTTGGCGCAAAACTGTGCCAATAAAATCATTAATGCTTGAATCAAGTAAATAAAATTGTTGTTGAATATTTTTTGATTTGCTTATTAAATAATCATTTAGTAATAATTCGTTAAATATCGAAGCAATTTCTGCTAAAAATATTGGATAAGAGCTTCTAAGTGGAGATTGGCATTTATTAGAAAAATACGAATGCATTGAATGTCCCATTTCGTGGCATAATGTATTGACTGATTCCAATGTTCCATCAAAATTCATCAATATATAAATTTTTTTCATTCCTGCTGCCCCACCTATAGAATAAGCTCCGCTTCGTTTAGATGGAACATTGACATAATCAACCCAACGTTCATCAATAGCTTTTTTAACAACTTCTGGATATTCATAAGGCATTATTGAAATAACATCTAATAAAATCTTTTGTGCTTCTTCTATTGTATATGAAGATTTAACATTAACTAAATCTAAAAAGTAATCTCATTTTTCTAATTTTTTATTGAATTTAGCTTTAAAAAATTGTTTTTTAGCATTGTTATATTTTTTAAATATATACATATGTTTTTGAACTGCATCATAAATAATGTTCAATAACTTTTTATCAACATGATCTTGCAACAAAATAGATTCAAGAGATGAATTATATTTTCTATATAAAGCATTAACTGATATTTCTTTAATATGTTGATATAGCAATCTTGCTAATGATTGTTTATTTTTTAAATATCCATTTAAATAATTAAAATAAACATCTTTTCTAACTTGTTCATCTTTATTTTTTAATAAAGCTATTCTATTTCCTTCGGTTATTTTTATTTTTTTATTTTTAATTAAAACATTGCCAAAATCAACTTCACTATCGCTTATTATTGAAAAAATATCTTCAGGTACAGGCGAACCACTTGCAGTATCATTTAAATAATTTTCAACATCATCCGACAATTTATGATCAAGTTCTTGCAAAGTTGCTTCTAGGTCTTTTTTTACTTCAATTAGTCTGTTGTCATTAATTCATGTTTTGATTTTGTCTTTATGTTGAAAAATTCTATTGGCTTCTGAACCTAACTTTTTAGCATATTCTTGGGATTTTGTTTCAAATAATGAAATTGTTTTATTTATTGTGGGATCAACAATATTTATATTTAATTTATTATCCAAATAATTAGAAATTCTATTGCCTAAAATTAAGAATTTCTTTGAAAGCTCAATATATTCAACATATTGGTCTAATGAATTGTATTTGCTATCTTTTATTTCAATTATTTTTGAATACAAATCAAAATATTCATCTTCTAATTGTTGATAGGTTTTATTACCTAATATGTCTTCAATATCTCATTTATATTTTTCTTCAACGTCACTATATGAATTGTATTTTTTTAACATTATTCCCCTTTATTAATATAGATTAAACTTTTTATATCGTATGGCAATCTTTCATGAGAATTAAAGCCATCTAATTCCATTAAAACAATTATTCTACTGATAATTACACCTTGTTCAGTTAATAATTTAGTAATTGCTTCCAATGTACCCCCAGTTGCCAAAACATCGTCAACTATGACGGCTTTTTGTCCTGGTTTAACTAAACCTTTTTGAATTTCTAAAATATTTCTACCATATTCAAGTCCATAATCGCAGCTTATAACTTCGCCGGGCAATTTGCCTTTTTTTCTTACCATAATAAATGGTTTTGATAATTTAGCAGCTACTGGTGTTCCAAATAAAAATCCTCTTGCATCAGGACCCACAATAATATCTGCACCTTCTGCTAATTTTGCCATTTCATCAATTGTGTAATGTAGTGCTTGTCCATTGGCTAATAAAAGCGAAATATCTTTAAATTTAACGCCTTTTTGTGGAAAGTCATCAACAGTTCTTATATACTTTTTTAAATCCATAAATTTCTCCTAACAATAAGTTGATAAATAACGAATAAATTTTAATATTATTTACAATTTTATTTTATAAAAAAATGATTTTATTTCAAACATTTGAATAAAAAACTAGTTAAAATAAATATATTAATTTGCTAAATTTTTTTTATTTATTGATAAAATAAAATTAACTTAAATTCTTTTATTTTAAATTTTAATAATTACTCGGAGGATAGAATGAATAAAATTAATTTTTTTGCATTAGGTGGTTTAGATGAAAACGGCAAAAATTGTTATGTTTTAGAAATAAATAACAAACTATTTGTGATTAACTCAGGAACTAAAATTCCTATTAACTCGCATAACGGTATTGATACATTAATTTGCAATTACGAATTTTTAGAAAAACGTCAAAAAGATATAGAAGGATTATTTTTGACTGATGTTAGAAATGAAACGTTTTCTGCTATTCCTTGATTATTAATGAAAATTAAAAACCTAAAAATATATACTAGTGCTTTTAACAAAATTGTTTTATTAGATAGAATTTCTAAATATAATATAGGTTCGAATACATTTGAAGTTAAAACAATAAATAAAAAAACAGCATTTGGCGGAGTAAATGTAACTCCTTTTGATCTAGCCGGAGGTTTGCCTGGACAACTTGGGTTCAATTTTGAAACCCCAATTAGCAATATCTTGTTTATGACAAATTTTGTTGATGGTGATTTGGGTCCTTATGGTAAAACCGATTTGCAACAAATTCAACAAATAATTAATAACAAAAAGGAATTACAGCTTTTAATAATGGATAGTTCAAGAAGCGCTTTTCCTGGACGTTCAATTGAAAAACTTTGAGTTTCAAGAAAAATTGAATACAAATTTAAAGAAACATCAGACAACCAACGAATTATTGTTGGTTTATATGATGAAGATATGATAACTGCACATGAAATTCTAATACTTGCAAAACGTTATAACAGACCGGTTATAACTTATGGTTTAGCTTATTCTCAATTAATAAAACTTGTTGAAAAGGTAAATCCAAATTCTCAATTTCCGGAATTCGTTGAATATCAAACTGCAAATAATGTTAAAAATGCCGTTATTCTTGTAACAGGTGCAATAGATAGACTTTACACAAGATTTGCAAGAATTTCTAGCGATAATGATGTTTATTTAAAATTGAAAAATGATGATGCAGTCATTATAATTGCTCCACCAGTTAACGGGTTGGAAGTAAATTATGCAATAACACTAGATGATATTGCTAGACATACTGCTAATTTAATTGAACTAGGCAATGATCAATATTATTCATGCAATCCTGCTAAATTAGATATTTTTAATATTATTAAAGCATTAAAACCTAAATTCTTTATACCAATTCAAGGTCTATACCGTTACCAAGTTGTAGCAACTCAAGTTGCCAGAAGCGCAGGTATGAACATTAGTAACTGCTTAGTGTTGCAAAACGGAAAGGTGGCTGAATTCAACGATACAAATTTAATTTCTCAAAAGCACGGAATTAGAAATGTAGGTGATGTTATTATTGACGGCTATGGTATTGGCGATATTTCTCATGAAGTTATTAATGAAAGAGAAAACCTAGCAAGAGATGGTGTAATTGCTCTTAGCTCATTAATAGATTATAAAAAGAAGAAACCAATCAATGAACTTCAAATTTCTTCATATGGAATAATTACGAAAGATAATAAAGAAGTTATTCACAAAATAATTAATGATTTATTCAAAAAAGAATTCGAAAATAAACAAACAAAAGAAATAAATTTAAAGGAAATTCAAGAAAAATTAAGAAAATCAATTAAGAGAAAAATCGCCAAAACTATTGACAAAGAGCCAATGATTGTAATAACTTTATATGAAATTTAACTTTTAAAAATTATAATTATAGTGATAACTAGGAATATTTATGAAAGTAGCAAAGGAAAAACAAACTAAAATTAAAAACCAAAAACAATACTTTTTTTCAAGAGACAGGGTGCTTTTATGAACAATAATACTTTTAGTAACATTGTTTTTTTTAGTTATATCTTTCCTTAATGTTCCTTGATTAACAACATTGCATTCATATTCATTTAATGTATTGTTTGGAATGTTCTCAATATTATTTTATTTATTAATTATTCTACTTTCAATATGAAAAATCTTTAATTTAAAATCAACGTATAAATATTGAATATTTAATTTTAACCTTGGAAGATTAATATTCTTTTTTATTAATATTATAATTTTGGGTTCTGTTATTTATTACACCATTCATCAAGTTGAACATAATTCAAAGAATTTTTTTACTAAATATTTTAGCAGTTGATTTGCTGATTTTAGTAATAGCCAAAATTATTTACTACCTCAAAAATACACCCCTGGAATTATTGGAACATTTATTAATTCAATATTAAATATTCCTGGAAAAAAACTTGGTTTTTCAATAGCTTACATAATAAGTATTGTTCTTTTAAGTTTATCTGTCATTTCATTCTTTATTAGTAACTCTTTCTTTAAAATGATATTCTCTCCAAAAAGCAAAAAGAACAAGGAAAAACTTCAAAATCAAAGCGATTTAAAAATAAAGGAAGAGGATATTGAAATAATCGATAACAAAGAAGTCGTAAATAGCAATATTCAAATTTTAGAGGCTAATTCTAACTCAAATAAAAATATAGACTCAACACAAAATTCAAACCAAAATAACAATCTTGATTTAGATCCTTTTGATGATGAAAAAGAAATTGAAGAAGATTCAAAACAAAAAGACAACAATGAGCCAGAGCCAAAAGAAGCTATTGACTCACCAAGCAATGAAAATTCTATAGATCCTACACTACAAGAACCAGATAATTCAAATAAAAATAAAGAGGTTAAAAAAGAAGATAAGATTTATAAAATCATTGAAGACGAAGAAGATTTATTCTAATTAAAAAAAAAATTCATTTTTTGCAAACATTTATTTATAAAAGCAAATTTCAATAACAAAATAATTTAAGCATTTTAGCTATAAACAAAGAGCATCTAAATTGCTCTTGTTTTTTTATATACAAATTATAAATTTACATAAATATTTTTTTAAAGGCTATTTTTATATATAATTTTTAAGTAAAAAATTTATGATTTAAGTTTGCTAAAAAAAGCAAAGTATAACTGATAGACTCCATATTTAACAAATAATAGTTTATTAATTTAAAATAGTTTTTTATTTTTTAGATGTATTGGTTCAATATATCTTAGTTAACTTTAAATATATACACCCCGAGCACAATAAATAATATAGAAAGGTGCTAGTAATGAATAGTAAAAAAAGTAAAAAAATTGTGGTTGCACTTTCACTAACATTGATTGCTTCTGCCATCACGATACCCGTTGTAATTTCGTCCTTAATTGAAAATAAGAATAATTGGGCTAATAAATACTATAGCCAAAGATCAGAAGCTAGTCATCTAGTTAATTTAATAGAAAAAAATAAGCATTTTTATTATTTTGCCCTAGAAGATCAAAACAATTTAAATAAGTTATTAAGTGAAATTAATTTAACCAGCAAAAATAGTTCTGCTGATTTTAAAAATTCATATAATCTACTTAACAAGACAAATCTTGATATCAACAAAAAAATAAATATTAATAAAAAAGAATTTGACAAAACTTATTCTGATATTTTGCTTTTAAGCTTGCAATTACAAGATTTATTCAATAAAATTATTGATTTAAAATTGAATAACAATATAAAAGAAGATGTTGATGGATTATTACAAAGAGTTGAAAAAGATTTAAATAAATGTAGAAAAATAGATGAAAACATTAATCTTATTGACATTAAAGAAATATACGCACATAGTTCAAAAACAATCATTGATGTTTCAAATCAATTAAATTCATATTATAAATTAATTAACAGCAAAGATAAAACATATAAAGGCATTGTTTCAGAAATAAAAGATCTTAAAAAAGATTATTTCAACAAAATTGAAGATGGAGAAGAATATTACAAATTATATGGCAATGAAATAAACCATCTTAAAAAGAATTTTAAAAATTTCCAATCAGTTGAAGATTACAACAATTATATAAATTACGCCAATTCAAAAATAAAGGAAATTAAAGAAGAAATTGTTGAACATAGAAAGCTAAGCCAAAAGATAAAAAAAGATGGAAATGATATTTTAAATAATGATTATGGTAAAAGTGCTTTAATAAGAAATTCATACTTCCAAGAACCAAAATCATTTTTATTAATGTGAAATACCTTCACTAAATTAGATATGTTAATAAAGGCAAAATTCATTAATGAACGTCCTAAAAAAATAAAAGAATTCCTAAAGCAATATGAAGAAAGGCAAGAATACTTTTATAGAATTAATGAAAATGTTCCAAACCTTATTTACACATGAGTAATTTATAATAACAAAATTAGAATTATAAAAGATTTCTATTTTAAAGATAAAAAAGAAAACGAAGAATTTAATTCATTTTATTACAAGGATAAAGATATAGACAATCAATTAAATTCATTTCTTAATGAAGAACGAGAATTTAAAAATAGGCAAGAAAGACTAGTAAGGCTGTTTTATGACAAGGTTATTCAAAACCCAAAAACTAACGAAATTGATGAAGAAAAATTAATGAAATTAGATGACATCCATTTTGACCCGGAACAAGATATTAGATATTTACACTACCATTTTGGAAGTCTCATAACTCAAAAGGTTAATGAAATAATTAGGTATTATAACCAAAAAGCAAAAACCTTATTAGAAAAAATAAAAAACAATAATAGTTATGAAACACAAAAAAATAGAATAGAAAAATTACTTGCTAAAAATATTACTTATGTAAATAATTTTAGAGGGGATTTCAACGATTACGACAAATACTATTGGTTAACATATAAATTTCAAAAAGACAACAATGGCGATATAATCCTTTCGCCAAATAGCAAAATCCCTGATTTTTATAAAAATGAAATTGAAGTAGCTCAATACCTACATGAACTTGATGAAATACTTGATAATTTAAAAAACATTTAAATATTTGTTTCTTTAATAAAATGGCATTATATGTGCCGTTTTATTTTTTTAATAATTAAGCCAAAAAATAAATTAGATTTTTATAATGCTTAAAATAAGTTACTTTTTAATTAAAACTTAATTTGCTTTTGCAATAAAAAACTAAAAATAATAAAAAAATTTTACATATTTGTTAAAATTAAAATGTTTTTTTACAACAAATTAAAAAAATAATACTTTTAGAAAAAAATAAGGAGAATATTATGAAAATGAGAAAGAAATTGATTGCTTTATCTGTGGCTTTTGGACTTTTAGCGCCTCTTTCAGGAACTATTATGATTTCGTGCAAAAACGAAAAAAGTAATGCTGAATATCAAAAAGAACTACAAAATAAATGAACAGAACTTACGAATCTTTTAAATTCAAATTCATTTAAAGAAGTTGAATCTAAGCTAGATCTTTCAAAATTAAATGCTCTTAAAGAAGAATATAAAACAATAAACAGCAAAAGCAATGTTTCAGAATTGAAGAATTTTATTGCAAAAATTAATGAAACTGTGCAAGATATAAATGCACAAATTTCAAAATTAAAAAACACAAATGGAAATGCAAGCCCAGAAAACAACAGCCAAAATGATTTAAAAGTAGAATTAGAAATAGCAACTAAAAAACTTAACATGCTACTTTCATCAAATGAAGGTAAAAACATCTCAATAAAAGAAGAAGCTAAAAAAATATTAAACGCCGCAAATAAAGCGTTAGAAAAATCAAATGAACAAGAATACAAAAACCAAATTAAGAAAGTAAATGAAACTATCAATAAAATTGAAGAAGAAATATCAAATTTAGAAAAAGGCCCAAAATCAAAAGAATTTACTAAATTAAAAGAATTAAGAGAAAAGCTAACAAATTACTTAGATAATAATTTCTCTGAATTTGCTTATAAAGATTTAAGAGCCGAAATCAAAGAATTAATTCAAAAACAAGAAAACGTTTCAATTGTTTCAAATGAAAAAGAAATAAAGTCAGCAATTTCACAAATAAATGATGCCTCTTGAATTGCCAACATCAAAAAATCAATACTAGATAAACAATTTCTTTATTTAGAAAATAAACCTAAATCTGAATTAGACGTAAAATTTTTAGCAGCGCATGACGAATTAAGCCAAGCATTGAATACCTCAATTTTTGAAAACATTGAAACAACAAATGAAAGAAATCTATTAAACAAATATATTAAAACAAATTTAACTAAAATTTTAGATGTAGAAAAACAAAAATATATTGAAGAAGTTAAAAACACTATAAGCTCTTTGTTGAACAAATATAATAATAATATAAATAACAATAAAGATCAAATTTATGAATTAAGTCGAAACATAGAACAATTAAATAAATTAAATCAAATAGAAAATAATTATGTTATTGACAAAGTCAAACTACTAAATCTATTAAATAAAGCAAATACTATAAAAAATGAAGGTTCATTTGCTAAAAAAGCAATTTTACTAGATGAGTTAAAAGACAGCGTTTCGGAATTTCTTGAATTTTATTTGCAATTAAGCCATTATCTAAGAATCTTTTTTGATAAAAATAACGACATTATTAAATTATTACCAGCAGATTCACAAACAAAAATTAATAATTATCTAAACGAAATTAAAAAAATTGCGGAAAAAAATAGTCAAGCCAAAGGAAACGAACCAACATTTGATGAATATATTTCAGCCTTTAAAGCACAAGAAAAAATTTTTAAAATTATAAAATCTAGTGATCTAAAATCAGCAGATGTTAACATATTAGAAAAATTGACAAATAATTTAAAAGAAATTAAAAAAGAAAAGAAAAATGATTACAACTTGGTTGTTACTTCTTATTTAAAAGAAAAAATAGAAGAAGCAATAACAAAAAATGAATCAATAATTGCCAAAAAATCTAATATCGAAAGTCAAAATATTACTGAAACGTTTGTTTTATTGCAAAAATTAGAACTTGAATTTGCATATATTAATTGAACTGAATTAAATTTGATTGCTAAAATTAAAAATAAATTAGAATTGTTAATAAAAATCAATAAAATAGATTCGAACGAAGAAGCAAAAATATTAAAGCCTTGAACAGATTCATTTAATAGTTCAAAAAAACCAGAAATTTTTGAATTAGAAAACATCTTAAAAGAATTAAGCAAATCTTTAAAGGAACTAATTAACAAATAAAAGTTATTTATTTAATAAAAACATTGTAATAATTTTGTGCAATGTCTTACTACTTTTTCAATAGAAAGAAACTTACATTACACAACCTATAAAAATAGTGTATAAAAAACAAGATAAAAGTTCTTGAACAATTATCTTGTTTTTTATATTATTAATTTTATTGCTATGAATATGCAATTAATTTATTGGGCTTTAATTGTTATTTCATCAATGGTTATGGGGTACACCGATCCAATAAATTTATAATAAGGAATTTCTGGGTCATAAAATTGAATAAATCCTTCAAATCTTAATTTCCCATCTTGCTTATAAATTCCTTTAGTTTTTGCTCCCCCTAAAAATGTATTAAAATTTACATTTACTTTGTCAAATAATTTAAATAAAGGAAGACTTTTATCAAAATATAATTTTCTTTCATCATGCGCATTTCCACTAGGCAAGAATGTTTTTATTTTATAAACTCTATCTTGATCAGTTTTTTCAAACATATCATTAATCATTTTATTAAAAATTACTTTTTTAACTTTTACAAGTGAACCGTCAAAAACCTTTTTATGTTCAGCAATAAATTCATCATTAAATTCGTCGTTTACAAAATAACTTCAATTTAAATATTCAATTTTGCTTCTACTAGTTTTGGTAATTTCTATAACATCTTCTTCCATTATCTTTTGTTTTATTTCATCACTTAATTTTGCATCTTTTGATTCAACATATTTGAATACTTCTTTTAAATAATTAGCGTACATATCAACTATCTTATAAATATTTTTAAGTTGGTTTATATATTTCTTACAATCTTCTTTATTTCTTGCATTATAGGCTTTTCTATAATAATATTTTTCTACATATCCTGGGTATTCGCCATAAACATCATCATTAACTTTTTTATTAATTGCCTTCTTTATGGATTCATTTGATTCAATTAATTGTCCACTATATGTTTTATTGAAATTTAGAGAGCAAATTTCATTTAGTTTAGATTCAATTTGCTTCTTTTGGTCTTGAAATTCTTCTTCAATTTTTAAAAATTCATCGCTTGGTGGCACAACTTCATGGCTTTTAGCTTCATTAATTTTTTGAGTTAATGATTTGATTGCTTCTTCAATAATCTTTGTTTTTGATTTAATGTCTGAAATATTTGATGATTCAGTAATATTTGCGGCATTTAATACATTTTGTGCTTCAGAATTATCAACTTTTTTTGCCTTTGCAGATTCAATTAATGTTTGTAATTCTTGTTTCTTAGCTTCAAAATTTTGTTTTACTTGAAGTTTGGTTGTATTTATTTTATTTTCTAATTGTTCTTTTGCATTTTTAAGGTTGTTTGAAACATCTTGTATTTTTGAAATAGTTCAATCTTGGGCGATATTTGCTTCATTCAAGGTTTTGTTTATATCATCAATGTTTTCTACTTCCTTTGCATCAGGGGTTGCAATTAATTCTTGTAGTTCTTTTTTTATTTTTTCAAAATCTTGTTTTGCTTGTTCAAATTCTTGTTTATTTTCTTGTTCATTAATTTTTTTAGTCAATGAATTGATTGCTTCTTCAATAATCTTTGTTTTTGATTTAATGTCTGAAATATTTGATGATTCAGCAACATTTGTAGTATTTAATACATTTTGTTCTTCAGATTTATCAATATTTCTTGCCTTTTCAGATTCAATTAATGTTTTCAATTCTTGTTTTTTAATTTCAAAATCTTGTTTTGCTTGTTCAAATTCTTGTTCATTTTTTTGTTCATTAATTTTTTTATTCAATGATTCAATTGCTTTTGAAATTTCATTAGTTTTATTTTTAATGTCATTAACTAAATCATTATCTTTAACTTCAATATCCTTATATTTTTCTTCTTCAACTTGTTTATTAATCTTATTGTCTATTGAATCTAGAAGTGCTCTCAATTCTTGTTTTTTAATATCAAACTCATTTTTTGCTTTATTTTTGGTATCTTCTATTAAATTTTGAAGATCTTGTTTAGCTTTATTAATTTTGTCTGTTTTTTCTTTAACTTCACTAATTGAAGAATTTTCTTCAATGTTTGTTGAATCTAAAATTTCTTTTGCTTTTGAAGATTCAATTCCTTGGTCAGCGCTAAAATCAATAAGATTTTGTAATTCTTGCTTAGCCTTTTTCAATTGTTCAATAGCAACGTTAAGTTCCTTATTTTCGTCTTTCGAAGGCGAATTTTGTTTCTTTTGTTCGTTAATTTTTTTCGTTAAAACATTGATTTTTTCTTCAATGTTTTTAATAATTTCGTTATCTCCTTGACTATTATCCCCTTTAAATTCTTTTTCTTGGTTAACTAGTCATTCATTAAGTTCTTGTTGTTCTTCATCACTTAGTTTTGAAATTAAATTTTGAAGTTGTGATTTTAGAGCATTAAACTTTTCTTTAACTTTTACAGTTACGGTACATTTAGATGCTAAAACAGGCACAGAGCCCGCCGCTACACACAACGCTAAAATTTGTAATGATAAATTTTTTTTCTTCATTTTAGTTATACCTTTCGTATTTATTTAAGCTTAGATATATAAAATTATATATATACATATATATAAAAATATAAAAAACAACAAAATTATAAAAATATTTTTAAACATTGCAAAAAAATATTTTTAGCCATATTAAAGTTAAAATCAATATTTTTCGCTAATTTTTATTCTTATATTGAGTTTTAATATGATTATGAGATAATTTTAAAGTAGTTAAGAGGAAAGGGAATATATGGAAGATCAAAAAGATTTTAATATTATAGATAGTGAAATAGCAGCATTTGGCAATGTAAAAAAGATCTTTGATGAAAATATAAACAATATAAACGATATTTGTAAAGAAGTAATTAAACAGGAATTTTTAGAAAAAAATTCAAATAATAAAAGAATTGAAGAAGCTAAAAAACAATACGAAATAGTAAGAGATTCTAATGAAAGAATTACTGCCGAAAAAAAGCTAAAAAAAATATCAGGAAGGAAATTTGCAAATTGCATAATTTTTATACTTTTCTTTATTCTAATAGGTTTTTGTTTTTTTTCATTTTTTGCTAACAATAAGAAAATAATTAAATCATTTGAAGAGTTTAGAAATAATGAACAATTAAAAATTGATGAAGCGTTATATAAAGCAAGACAAGCATACGTTGAAACCTTTTCATTACAAACCCTTGAAGATATAAAAAATAAAATTTTAAACAAAATGGGAATAAAAAAAATATCTCAATACGATGCTGAAGTTGGTGATTTTTCGCGTAGTTTTATTTATGATAAAAATTTTTGAAATTTTAATAGCATAAGTCAATATAAATTAAGAAATTCTTATATTTATGACATTTTTTATACTTGCAAATGAATGAAAAATGTTGTTACTAAGGCTTCAATTACCGTTACTGAAGAAGATGCTAATGGTAATACTTATTGAGAAACATATTGAGCATATCATACCGAACCTACACCTGTTGTAGAATATTTTAGAAGTTTAACCATACCTTCAAATTACAAGCAAAAACTATACTTTGAAATTATAGAAAATAATCGAAGTGAAAAAGAAGTAAAAAGACTTTTAAAAAATGAAGGCTTCCACCTTGAAAATAAAGAAGTCTATCAAAATTTTGATTTTGCATTTTCTGATGACCAAAAAATTGATTTTTTAACTTATTTTGATGTTAGAACTCAACAAAACCTAATCAATTATGCCCAATATATGAATCAAGAAAACATGCCGGTGTTTTCGATGATAAAAAATGGCAATTGGTTGGTTTCTAAAAATACGATTCAATCAAGTTCCTTATTATTTGAACAAGTTCAGGATTGGATGTTAAGTGTTTTGGATTTAACTGAAGAACTAACAGTTGACTTTGTCACGGAATTTTTAAGTTCAAAAATTATTGAATGTTTATTGCCAATATTTAATTTTATTTCCTTAGTATATACAAATAAGTATATTGCAACTGAAAATTTAGAAAAAACAGAAAATAGATATGAACAAGACCTTGTTGTTAAACAAAATAACAATATTCAAAATTGCCCTCTAGACATTCACTCATATTATAGAGACATGACATTATATAGTGGGTTTTATAAATTTAACAAGCATGCAGATATAACACCAATTTGCGAATTTAAAAATCTTTATAATGACCCAGAATCTTTGATAATATCGGCTACATTTGATTTGATTTCATATACAGCTAGAGACGCAATTGCAATTGAATATACACACGGTTACGCCGTTGAAGTACCATATGTGGCATACGATGAATTTAGAGAACATAAAGCAATAATTGTTGTTCCTTATAATATAAAAACAGAAAACGCCAAAATATTTGCAAATAGTCTTATTGCTAATAATATTTATGTAAGTGACGATCGCATAGATGAAGAATATAGCAAAATTATTCAAAAAAATAAAATTTCATTTAATTTAGAATCATTAAAGGACTTTGATGAATTTAAGAAAAATATTAGGCTTATAGATGAATTATTTAGTCAGATTCCTGAAATAAAAAAGATTCCAATACTACTTGATAATAGAGGAATGGCTTTCTTTATAAATGAGCCAAAATTATTTGACATCAATTTATTAGTTAAAAAGCTTAATAATTTTTATTAAAATTATATTAATCTCTATTTTTGTAATTAAAATAAAAATAGATTACATTATAATTTAATTATTAATAATAAGTAATAAAGGAGCAATATGTTATTTGATTCAAGAACCCCACAATCTAGTGAAGGATTTAAACCAAACGTAGATAATAGCATTAAAAAACCAATTCCAACAGGTGTTGAAAAATTCTTTTTTATATTATTTTTTATACTTACAATAGGAATTTTCTATTTTGTATATGTAGGAAGAAAAAATGAATTAATGCGTGATCAAAACGAAATTCAAAATGCAAGTTCTCTAATACAAGCAGCAGAAAAAAGGAGACGTGCAGTTCTAATTAAAATGATGGATAGTTTAATTGGCTATAAAAATTTTGAAAATGAAACATTAACTAAAATAACTCAATATAGATCAAAATTATCAAATATCGATGTAGACAAAACTTCTCCCGTTGAATTGAAATCACAAATTGACAGCATCAGAGGTGCTTTAAACTTTCAATTTGAACAATACCCAGATCTTAAAGCAAGCAAATTGTATTTACAATTTTCAACTGAAATTTCTATGCAAGAAGATGAAATTTATGCAACAATAAGAAACTATAATATGATTGCAACATCATTTAATTCAAAAATATATACATTTTGAACAAATTGTGTTGCTCAAAAATTAGATTTGTACAATGTGGCTATTTTTCAAGCATCCGAAATAGAAAGAGCAGATGTAGATACAAGCGAACTTAGAAACTAAATGAGCCGAAAGGCTTATTTTTTTGCTTTATTTTGCTATAATATAATATATTAATTAATTAATATATTACAACATGCGAGGAAACATGGACAAAATAGAAGAAATACATAAATATAATGCCGATAATATTCAAATATTAGAAGGTTTAGAAGCCGTAAGAAAAAGACCCGGCATGTACATAGGTTCAATAGGGTTCAAGGGTTTGCACCATTTGCTATGAGAAATAGTAGATAACTCAGTCGATGAAGCAATGGCGGGTTTTGCTACTGAAATTAAAATTAAATTGTATCCAAATAATGTGATAGAAGTTGAAGACAATGGTCGTGGAATGCCCACAGGAATTCACTCTGGAACTAAAAAGTCAGCTGTCGAAACAATTTTAACCGTGCTACATGCTGGGGGTAAATTTGATGGATCAAATTATAAAGTTTCCGGAGGATTACATGGTGTTGGTGCATCAGTTGTTAATGCATTAAGTAGTGAATTTGAAGTATGAGTTAAAAGAGATGGTAAGTTACACTACCAACAATTTAGAAATGGTGGAATTCCTGTTAAACCTTTGGAAGTAATTGGAAATGTTCCTGAAGTTGAAACAGGAACAACAATTAAATTTCACCCTGACTATACCATAATGGAAAAAGAAAATTTTGACTTTGATACAATTATTGACCACTCCAAACAAATTGCTTATTTAAACAAAGGTTTGAAAATAACCGTTGAAAATGTTGAAAAAAATATCATCAAAGTTTTTTGTTTTGAAGGTGGACTAATAGACTATGTCAAAGAACTAAACAAAGGTAAAAAATTAATAGTTCCCGAAGTTATTTATGCAGAAGGAGTTTTTAACGATAAAAACTTTACAAATGGACAAGATGTAATAGTAGAAGTTGCAATGCAATATAATGAAGCCTACACAAATAGTATTGTTTCTTATGCAAACAATATTCAAACAATTGATGGTGGAACACATGAACAAGGTTTCTATGATGCATTAGTAAGAATTTACAATAATTACGCCGAAACAAATAAACTATTTAAAACTAGCTCAGAAAAAATAACAAGAGAAGATGTTAAGGAAGGTTTAGTAGCAATCATTTCTATTAAACACACAGACCCAATTTTTGAAGGTCAAACTAAAGGAAAATTAGAAAATAAAGATGCAAGAATTGCCACAAATAAAATTCTTTCAGACTCGCTAGAACGTTATTTGAATGAAAACCCAGAAATTGCAAGAGCAATAATCGAAAAGTGTCTTCTTTCGCAACACACAAGGCTTCTTGAAATAAAGGCTCGCGAAGCTTCTAGAAAAGGTAATGGTTTAGATTTAGGTAATCTTCCTGGAAAATTAGCAGACTGTTCATCGAAAAATGCAGAAATTAGAGAATTATTTATTGTCGAAGGTAATTCGGCCGGAGGTTCTGCTAAAATGGGTAGAGATCGTTCTATTCAAGCTATTTTGCCTCTACGTGGTAAGGTTATAAATGCAGAAAAAAATTCGTTTGCTTCTGTCTTGTCAAATAAAGAAATTGCAACAATGATTCACGCCTTAGGCACGGGAATAAATACAGAATTTGATATTAACAAATTAAAATATCACAAAATTATCATTATGACAGATGCCGACGTTGATGGAGCGCACATTACTACTTTATTATTGACATTCTTTTACCGTTATATGAAACCTTTAATTGAATATGGATTTGTTTATTTGGCACAACCTCCGCTATACAAAATAACTAGTGGAAAAAATGTTGAATATGCATACAATGATTTACAAAAAGAACAAATCATGGCAAAATTAGAAGATAAAAGAAATGTTGCTATTCAACGTTACAAAGGTCTTGGTGAAATGGACCCAGAACAACTATGAGAAACAACAATGGACCCAGAAACTAGAAAAATGCTCCAAGTTCAAATAGATGATGCAGCAATTTGTGATACAGTGTTTGCTACATTAATGGGAGAAGAAATAGAACCTCGTCATGATTTTATTCAAGAAAACGCAAAATACGCAAATAATATAGATATCTAATATGCTAAAGCCCCTAACTAATCAAGGTTTCACAAACAAAGTATTTTATGATGATGAAACTAATAGATTTATAAAAATAAAATCATATGATGGATTTAACCATAAAAGCGATGCATTCTTATTAAATAATTTAGATTTTTGTCCAAAGATATTTGTTGATAATAAAAAAGAACTTCAAACCGAATGAATTAATGGAATTACATTAAACGAAAGTCTTTTGACAGATGATATTTTAAAAACTATTGGAAAAAATTTAATCACTTTGCATAATTCAAAATTGAAATTTTATAAAGAAAATCAAATTGCTAGAAGATTTAATATTTATAGAAAAAAAATTTCTAGTTTAAATAGAAAAATTCCAATCCTAGATAAATACTATAAAAAAATTAATTTGTTTTTAAGAAATATTGACAATTCTGCCCCTGTCCATAATGACCTATGACTATTCAATATGATAAAAGTAAATAATAAAATTTATTTTACAGATTGAGAGTATGCCACAATGGGCGATGTACATTTTGACCTTGCCTATTTTATTGAATCAAGCAATCTAAATGAAAAACAAGAAAAAGTTTTTTTAGATGCTTATGGTGATGATTTTGAACCCAAATATTTATTTATTCACAAAATTTTAGTAAATGCTTTAATTGTGCTATGAATAAATGCCCATGAAATACTTCCATTTGATGATAGTCTATATCTAAATAGAGTTGAAAAATACATGGAGCAATTAGAAAAAGAAAAAGAGTAATATGTTAAAAAGAGATTGTTGTTGTGGTTGCAAGTCAGCTGAGTTCTGCACATGCAAAAATAAAAAATCATGTATTCCTAATAATTTATATGAAAAAATTAGGAATGTTTTTGGTTATGAAGTTTTTGAAAAACTTAATAATTTAAGACCTTATTTTGATGATTTACATAGTAGCACATATATTGGAAAACTAGAAGATGTTTGGGTTCAAATAAGAATTCCTTCTGACAATAAAATAAATTATGACAACGAAACAAAACTAGTTGAAAAATTCAAAGATTATTTTTATTACAAAGATGGATATATTATAAAAAAATGATTCCCCGGAGTAGACTTGTTTAAAGTTAAAATTGATAGTGGAATTAAAAAAGCAATATTTAACTGCGTCAAAAATTTTCAAAACCTTAATGTAGATAAGATAGAAAAATTCGATTGATTCAAATATCCTATTCAAGACGCTGAATATAAAGCATTAGTTAAAAAATATTCAAAAGAACCTTTAGTACTAAGCCATAATAACCTAAAACGTCAAAATATTCTAGTTAATAAATACGGCTTCATTAAACTAGTAGATTTTGAATATGTTGCTTTAAACAATAAATATGTAGATCCAGTAAGTCTTTATTTATTTTTGGGTATTCCAAAAGAAGATATAATTGATTTCTTTAAATTAGACCCTTCTGTATTTGATGATTTTGTTTTTTTAATGAGAGTTTACAATGAGGCAATGTATTTAAATGATTATTCAAAAAATAATTCCAAATCGTTAAGCCCTTTTGATTCAAAGAGTTTATATAGCAACAAGGATTTCTTAGAATTAAATAGATTCATTGTTCAAAAAAATCATAATAACTTTGACAATAAATTAAATATTTCTAAAATTGAAAAATTTTATTTTGTTCCACTTTGTGTTTACGAAGATGAAGATAGAACCATATGAAAATGAATCAATTCTAAACAATTAAGTTCTTTCAACAACCATCAAATTAAAGTTCTTGCAAAAGCAATGAGAACGCTTCATGATTCTGATGTAGAATTTCCTGAATATATTTTGTCAAAGAAAATTAATTGATATTTAGATCATATGGAAATAAAAACCTTATTAGAAGATTTAAAAGGCAATAAAAGAATCAATGAAATTATTAAATGAATCAAGCAAATTAAACCAGATGCAAATTGTCATAATAATTTAAATTTCAATAACATATTCTTTAATAGTAGCGACAATTTATATATTATTGATTGATCAGTTGCATATAGAAACAATCGTTATTTAGACATAGCCTTCTTATTTGAAAATACTCAAATGACACCTGAATTAGAATCTTTATTTTGAAAATCTTATGGTATGATATGTCCAAAAGACTTTTATAAATATAGAATTATTGTTCACTTTACAGCATATTTATATAACAAATTATTAAACACTGATTTTAATGCGGCTAAGGTTAACACTAAAAGAATAAATGAAATATTTGAAAAGTTAAACATTAAAGACTAAGGACTAATAAATGAAAAAAGGCAAAGCAAAAGAAACAAAAACTATTGACGTCGACAAGCAAGAAAAAAAATCAGTTAGAATTCGTAGAGTAAAAACCGGATTTCTATTTTCAGGAATTTTAATTCCTTTAGTTTGCGTTGTCGCAATACCACTTTCATTAAACAAGAATTATTCATTAAAACGCACCAAATATTTTACTGACGATAGAACTTTATATTCAATTGACAAAAACACTATAAAAGAAAATAACAAAACTTATAAAATACTTACCCTAACATCTAAAAGTCTTAATGTTTCTGATATCATGAGCGAAAGCGCAAGATATATGAATATTCCGCAGAAATCGTTTAATAATTCCGTTACATATTCAAATAAAATCAATATTAGAACAAATACCAACTTGTTAAAAAATACAAAAATTAATGAAACAAAGGTTACAAAAATAACAAATAGTAACCAAACTATTTTTAAATTAGATTTAATTCAACTATGAAATGAAATAAATTCAAAATCTAATAATAAAATATCTGAGTTTGATTTTTTAAGCAATATCGAATCAATAAATAATGCTATGACTTACTCTTATATAAAAAATAACAAGCTCTTTTACTTTGACAATAAGCAAGGATATTCTGGATATATTATTCAAAAATTCTTTGAAAATTTTGATGTTGAGATTCGTAAAGAAAATCTTAAAAATAAAAATGTTAATGTTTTAGAAGCTTTTATTTCTAGCACTATTGAATTAAACGAATCATTAACCGAAATTAAAAAAATTACTTTTGATACTAAAATTACATTAAAATATGAAATATAAAAACAGGCAATCGCCTGTTTTTATATTTATATATTCTTTGAATTATTTTATACCGTAGTGAATCTTTGTAACTTTTTCTAAAGTATCTAGAGGTAAGGCACTATTCTTTAATAGAACGTCAAAGAATTCTTTTATGTATTTGTGGTTTTCTTTTTCAACAAAGTCTCTTCTTGTTAGTCCTAAGTGTTTACGTACTTGGTCATATAGAGATAGCATCTTTTCTTTACCAGCATTGTATGAAATTGCTTGTCCAGCTAGATTTAGGTAACGTTTTGATTCAGACATAATGTCACCAACACCTAATGCTGAGTTTGCTGACATAAATCTTCTTACATCATTAATTGATGCATTTGCAGGTAAATCAGATTTTGCTTCAATTCCTTGTCCATGGTATGCAGGGTCAACTGCTCTACGCATATTACGTAATTGTGCTTCGTTCAATGCTCCAAAGTATTGTAACATATTTGTTAATTTTACTGCTCTTTGTGCATGTTCTTTTTCGTCTGTTTTATCTTTTTTATCAGTTAATGTTCAATATACTCCACCATGTAATTCTTTGATTTGGTTTATCATTTCTGGAGTAATTTCATCTTTTGTTTTTGCTGTAAAGAAGCTTGTTATTCCTTTTGCATGGCTAAAGTCAGTTGGTTTTGCATAGTAATCGTTATCTTTGTTATCGTAGTCAGGAGTTCCGTAGTATCCTGCTTCAATTCCAAATCATTCCATAAATAATGCTCAACCTTCAACGTATGAAGTAAAGTCAAATGCATTTGCACCTAGATTTTCATTGTTTTGACTTGCTAGGTGTTTACGTGCGTAGTAAATTTGGTTGTGGTGTCCCATAATTCCTTCGTGGTTAGCGAATGAAGTAACTGATCATTTTGGCAATGAGTAGTATGGGTCACAGTTGAAGTTAAATGCACCTTGAACAGAAGTTACGTTTCCATTTGCATCTAAGATACGTGTATCTGAGTTATATGCTCCAACACCTTCTTGATCACGTCTTTCATATAGATATGTTACTGCCTTGTAGTCAGGAACATCTTTACCGAAGAATGATTTTCCATTCTTAATGGTTGACTTCATGAATTGGTCATAAGCCTTAAATGCTTCTAAGGCACCATAGTAGAATTGTTCGTTTGTGATTGAACCGTATTCTTTAGCCTTGTTTTCATCTCTAGTTAAGATGTCATATCCATAAGCACTTAAATTGGTCTTGTATTGTTGAAGAGTTGGATCATCAAATAATGACTTCTTGTAGGTATCTGTGTAGAAACTCTTATCTTCACGACCAAAGAAGAATTGTTCTTGGTTTAATCATTTTGCAAAGGCTTGTAAATCGATATTGCCTTTTGCATCACGAATTGTTAATTCAAGATCTTTTACTTTTTCTGGTCCAATACCATCAGCATCATATTTAATTGTGGTTTTTCATTCTCCACTTTCACCAGCTATTAATTTAGCAACTTCTGATGCAATAGCAGTCATGTTCTTTGAAACACCTTTTGATGTTTTGTATCCTGATTGATATACATCATTTGGAGTGTCTTCTGATGTGGTTGCCATTTTTGTGATTTGGTCATAAATGGTCTTTCCACTTTCAGTTTCATCACCGCCAACTTGTGAGAATCCAACGCCCTTAGCTTCTAAGTCAAATTTGGTAAAACCTAAACCATAAACTGATCTTTCAGTAAGTTTTCCGTCTGCTTCTTTTGAAACTGATATTGTGTTTTCTGTTTCATTGTAAACATTGTCTTTATACAATTTAAGTTCTTTTAGATTTTGACCTAAACCATGTTTTGAAGCGCTATAGTATTCTGTAGCATAGTATTTTCAAAATTCTCTTAGATCTTTTTTATCTGTATCTGTGTTTAAATCAATTAAATCTGAAACACTAATTTCTGTTAAAGTGTCATCGTCTAAGAATCTGTTTAATTCATTTGCAAAGAATTCTCTAAGTGCTTCTTTAACATTGTTTTTGATAACAACTTTTGATAAAACTATATCTTTTTTAACAGCTTCTTGTAATCTTGCTTCTGCTTTAGCTCAATCATCTTGATCTAAAATTGTTCCATAGAATGAGTTTGCAGCGTAAACGTATCCTGCTCCTCATCCATATCTAGATAGAACATTACGTCCACCTGCTAGATAGTTCTTTTGAACCATTTGTCAGCTGTAAATTGTTGCATCTTTTCATGCTGTTGTAATAGCATTTAAGTTTTCTTCTTTGATTAATTCTAGCAAGTTAATGTATTTTTCATAGAAGTTAGCAATTGCTTCAGCTTGTTCTCTAGTTGGTTTTTCAAAGTCAACATCTTTTAATTCTTCAACATCAGCAGGATTGTTTAATGCTGAGTCTAGTTGGTTTCTAACTTCAACGATATAGTCAGCTACAACGTCATATATTTTGCTATCTTTCTTTGCTGATAAGCTTGAAACTGCAACCTTAGAAAGTTCAAATTTTTCTGTAACTTTATCGTTTATAAAGGCTTTAGTTTCTAGAATTTTACGTTCTAAAACTTTTATTTCTTTGCTAATAGCTTCCTTTTTATCTTCGGCAGCAGAAGTTAATTCATTTTCCTTAGCTGTTTTCTTAGCATTTAAGTCATTTAAATCTTTTTGATAAGCTTTAATTTGGTTTTCAACTGACTTGATGTTTAAAACACTTCTTCTTACTTCTTTATTGTGGACACCAGTTGAAATTCCTCATCCAATTAAAGGAATTCCGGCAGTTACAACTGCGGCTCCAAGAACAGCAGCGGCCACAAAAATTTTAGATTTTTTTGTCACTTTTTTATTTCCTTTCACTTAATATAAATGTATATACGTACATATTTATATTTGAGTTTTTTTATATACATATATACCTAAAAAAAATTGTTGGTTATTTTAAACCTATAGAAAATTATAGAATGTATTAAATCAATGTTTAAATATATAATGTAATAATAAAATTTAGTAAAAAAAAATAACCATAAATACTTTTAAAAATGAAAAATAAAAAACAGAAGATTTTCTGCTTTTTGGTTTACTTGGTATGGCGGATAGTAAGGGATTTGAACCCTTGAATGTTAGAAAACATTATTAGTTTTCGAGACTAACCTCTTCAGCCTCTCGAGCAACTATCCGTTAGTTTAATATTTAGATTTTTATTTTTTTGACTAAATTAATCTAATTATTAAATCAAAACAATTATAATAAAAATGAGTTAATATACGCAAAAATAGTAAATGTTTATTTTATTAACTTATTAAGTATATAAAATAAATTCAAATAATATTATGAAAGTAAAATTTTATATATTTATGGTTTAACTTATTATTTATAAGTTTAGTTTAATCAACTTGCAATATTTACTATTGCAAATAATAATATAAAAGAGGAAAAATGAAAAAAATAATTCTTACAATTATCGATGGACTTGGCCTAAGAAAAGAAAGGCAAGGAAATGCATATTTGCAAGCAAAACACCCTTGCTTTGACTATCTTTTTTCAATGTGCCCTAATTCTGTTTTACAAGCATCTGGTCAATATGTTGGTTTGCCAGAGGGACAAATTGGTAATAGCGAAGTAGGACATTTAAATATTGGCGCAGGTAGAGTTGTATATACGGGACTATCTTTAATTAATAAGGCAATTGAAAATAATACTTTTAAGGATAATGAAATTTTAAATGATGTCATTGATAAAACTATTAAAAATAATACAACATTGCATGTAATGGGTTTATTAAGCAACGGCGGTGTTCATTCATTAGATTTACATTTGTTTGAAATTTTAAAATTAGCGCATAGTAAAGGATTAAAAAATGTAAGTGTTCATGTGTTTGGTGATGGAAGAGATGTAAAGCCTCAATCAATAAAAAATTCTCTTGAAACCTTAAAAGATTTATGCCAAAAATTTGGATATAAAATCTCTTCTATATCTGGCAGATTTTATGCAATGGATAGAGATAGTATATTTTCAAGAAATCAAGAGGCATATGATGCCATTTTAGGACAATCAAAAAATGTTATTGAAAACATTGATGATTACATTGAAAGTCAATATAAAAAAGGAATATTTGACGAATTTTTTAAACCTGCTCAATTAACAGATGGCTCATTTGTTAAAAATGGAGATTCGATTATATTCTTTAACTTTAGACCAGACAGAGCAAGACAACTAAGCCATATGTTTATAGGTTCAAATTTATACACATACGAACCAAAAAACAAAGTACAAATCGATAATTTTGTTTCATTGATGAAATATGAAGGTATAAATAGTAAGGTCGCCTTTAAAGAAATGGAAGTTGTAAATCCACTAGGCAAGGTTTTAGAAAATAATGACATTAAACAATTAAGACTAGCAGAAACTCAAAAATATGCCCATGTAACATTCTTTTTTGATGGAGGGGTTGACATTGAATATAAAAACGAAAATAGAATTTTAGTTGATAGTCTTAAGGTTGATTCATTTGCTGATTATCCACATATGTCGGCCAAAGAAATTACAGATTCATTATTGAACAACATTGAAAAAAATGATTTTATAATAATGAATTATGCAAATCCTGATATGGTGGGTCATACCGGCAATTTAAATGCAACAATTGAAGCAATTGAATTTTTAGATTCACAATTCCAAAGAATATTAGAATATGTGTCATTAAATCATGAAAATGTAACTTGGTTTATTACAGCAGATCATGGCAATGCCGAAATTACAGAAGATGAAAATAACAAACCAGCAACAAAACATACAACAAGCCCAGTAATGTTTATTTGTACGGATAAGAATGTTAATTTAGAAAATGGTAGTCTTTGCGATGTAGCTCCTACAATATTAGATTATTTAAAAATCAATAAACCAAAAGAAATGACCGGAAAATCATTGCTTAAATAACAAAAAAAATAGATTCATTATTCATTTGAATCTATTTTTTTGTTATTCATTTTCTTCACTAAAAGATGAATGATATAGCTTTTCATAAAAACTACCTTTGGCCAATAGTTCTTTGTGTGTACCTTGTTCAATAATTTTTCCATCTTTAACAACTAAAATTTGGTTAGCATTTACTATTGTTGAAAGTCTATGAGCAATAACAAATGAAGTTTTTCCTTTCATTAACTCTAACATTGCATCTTGAATATTCTTTTCTGTTCTTGTATCTACATTAGATGTTGCTTCATCCAATATCAAAATATTTTTATTAGCAATTATTGCTCTTGTGATTGCAAGCATTTGCCTTTCACCTTGTGAAAAATTGCTTCCGCCTTCTTCAACTATTTCTTCGTAACCGTGTTCAAGTTGTCTTATAAAATTATCAGCCTTTGTAATTTTGGCAGCATTAATAATTTCTTCATTGCTTGCACTAGGATTAGCATATCTTAGATTTTCTAAAATTGTTGTTTTAAATAGAAATGTATCTTGCAATACTATTGAAATTTGATTCCTTCAACTAGATTCATTTATTTCACTTGATTTAAAATCATCAATTTTTATTTCTCCTTCAATAGGCAAATAAAATTTGCTTAGTAAATTTATTATTGTAGTTTTGCCTGCCCCGGTAGGTCCTACAATAGCAAACACTTGACCTTTTTTTGCTTTGAATGAAATATTTTTCAATTGTAAATTATTCAAAGAATTATCATATGAAAAACTTACATTTTTAAATTCAATATCACCATTCACATTTGATATTGTAGCAAGTTTGCTTTCATCAACGGCCGGATGCATTCTAATTAAATTTAATACTCTTGTTGTTGATATTGTTGCTGTTTGAATCATTGAAATATTTTCAAAATTTTTAATTATCTGACTAGAAGTAATTTTTATATACAAAATAAATGATGTCAATGTACCCACTGTAAAATTACTATTTGAGCCAGTGCCAATTCCAGACAATGCAAATGCTCCGCCAACAAAAATTATTATTAATTGCATTATGAATGTTATTGCACTTCCATATGGATAAACTATTCCGGCATATATATTTGCTTTCAATGAAGGTTTATATAATTTTTCATTATATAAATCAAATTGTTCGTTAATAATTTGTTGACTATTCAATGAATTTATTAATTGATGTGCTGCTAAATTTTCTTCAACAAAACCATTCAATAAACCTATATTTCTTTGAGTCTTTCCAAATAATGGAATTGCTTTTTTGATTAAAACAAAGGCCAATATCAACAAAACAGGAAATAAGGCAATATATATAAGTGTTAAATATGTAGAATAGTAAAACATAAATCCAAGCGATGTTAAAGCACTAAATATCGCTGCTAATAACGAGTTTAAAGTTCCACATATTCCATATGAAACATTATCAATATCATTTGACAATATTGACATTAATTCTCCTGAGCTAACAGTATCAAAAAATTGGATTGGCATTTTCATTATTGATTTATAAGCCTTACTCCGCATTATTTCTGAACCATAAATTCCTGCCTTGACTGCCATTGCTGAACATATAAATGAAAATACTTTTTCTAAAATATAGCCTAAGATCAATATGCCGATTGCAATACCAAATTCAAATAAACCATTAGAATTACTATTTCAAAACTTAAAGTTTAAATAATGGTCAATAATATAGCCCAAACCGTATGTGGCGGCAGTTAAACACAATGAAGAAATTAATGTAATTATTATTATTAAAAAAACATATCCACGTTTGTAATTTAAATATTTAATTAGTTCTTTAAAACTTTCTGATTTTATTGCGTGTTGTTTTTTTCGAAATGCTTTTCTTTCAAATTTAGATACTTTCTTTAAATCAATATCAACTTTTAGTGGATCAAGAATATCCCTATTCATTATTGCCCCCTAACTGTGAATATGCAATTTCTTTATATAGACCATCTCTAGAAATTAATTCCTCATGATTGCCACGATCAATAATTTTTCCTTGGTTTAATACTAAAATTTCATCTGCATCAATAATTGCTGAAATTTTTTGGGCAATAATAACTAATGATATTTTTTCGTATTTAAATTCTTCTTTAATATTGTTTCTTACCATTGCTTCTGTTCTAGCATCCAATGCCGAAGTAGAATCATCTAAAATCAATATTTTAGGTCGTTTGATCAAACCTTGGGCGATAGATAATCTTTGTTTTTGACCGCCTGATAAATTTGCGCCCTTTTGTTCAACAACATGTTCAAATTTTTCTTTAAACTTATTGATATATTCAAAGGCACAACCATTTTTTGTTGCTAATTCAATCTCTTCATCAGTGGCATTAGGTTTAGCAAGCAATAAATTGCTTTTTATTGTGCCACTTAATAGGCAAGGGTTTTGATAAACGTGGGAGATATTCTTTCTCAATGAATCTGTATCAATTTCTTGAATATTATGCCCGTCAATTGTTACTAGCCCTTCTTGAGTTTTAAAATCTAAGTTTAATAATTTTGCAATAGTTGATTTTCCAGAACCAGTAGGGCCAATTATACCCAAAACCTTACCAGGCTTTAAAGTAAATGAAATATCTTCTAAAACATTAGATTTGGCAGATTCATAATATTTAAAAGATACATGGCTAAATTCAATTTCTCCATTAACAATTAGCTTATCAGATTTGATTTTTGCAATATCTGGCTTTTTGTTCATAATTTCAAAAATACGTTTTGAGGATACATTTGCTTTAAAAATATTAAATGTAGTAAATGATGAAGCAACAACTCCAAATGTAACAGTCATAACGTAATTCATAAATGTTACCCCATTACCTATTGATTTTGAGAAATCAGCAATATTTTCCTTGCTGGTTTGCTTTCCTACAAAAAATACAATGAAAGCAATTGCTATATTTGAAATTATTTCAATAAAATTAAATGTTATAGTGAATATTATTCATGATTTACTTGTTATAGTTCCCCAATTTTTATTAACATTTTGGAATTTATTTCATTGAATTTGTTCCATGTTATATGATTTAATAACTTTGGCGCCTAAAATATTTTCTCTACTTTCAATGTTAATTTTGTCATACATTTCTTGTTCTTTTTTTTGAATTGGATTTCAAATGACACCCGAAATTACCATAGTTAAAGTTAATAGTGGAATAACGACTAGAAATATCATTGACAATTGAAGATTTGTTAATAAGGCAAATACTAAACCTAAGACCAGATTAAATGGCCCTATAATTAGCATCCTCAAAAAAGACATCAAGCCGGTTTGAATTCTACTAATGTCTATTGTAAATCTTGTTAGGATCTTGGCGTTTGAGAAAGCCTCAATATCTTTTTGAGAAAGTTCGCCAAGATGCTTTCATAAACAACTTCTTATTTGTTTTGATCCTGCTTGAGACACATATGATGCAAGCAATGAAGCACACATTGCCGAAATAGTTCCTATTATTAAAAACAATGCAAAATATCCCCCCAAATGCATTAATGCACTCTGGTGGCTTTGACATAAAAAATTAATTCTTAGAATTTCAATTGTTATTGGTCTGTTTGTTGACAAATTATTTTGAATTAAATATGCATTTTCACCAATTAACCTTGTCAATTGTCCCAATAATATTGGCAATACTAAAAATGAAACTACTTGTAGCAATGTTAAAGATATAGTTAAAAATGACAAACATTTGAATTTGTTGGCCATTAATTTATAAAGTTTAAACATTGTTTCCTCTTTCTTTTTAAATTTGTTTTAATTGTTTAATTTAAAAAGTTACCTTGTATTATCATCAAAAAATTGTTTAACTTCTTTTGCGATATAAAGTTCTTCATCTGCCCTTATTATATAAATAGGAATTGAAGAATTTTTAGTTGAAATTAATTTGTATGAATCAAAATCTCTTCCTTTATTTAGTTCGCTATCAATTTCTAATTTAACTAAATGAATTTTATCAACAACTGCTTGGCGAATATATTCATCATTTTCACCCACACCCGCCGTAAAAACAATAGCATCGATTTTTCCTTCAATCTTATTCATGTAATTAATTAAATAATCTGCTATTTTTCTTACATATAAATCTATTGCAAATTTTGCTCTTTCATTTGTTTTTTTAGCTTCTTCAACGTCACGAATATCAGAAGAAATTTTTGAAACTCCTAATAATCCTGATTCTTCATTTAACATTTTTATTGTTCTTTCAATATCTCAATTTTTATGTTTCATAATATATGGAATAATTGAACAATCAACATCACCAGAACGAGTTCCCATCATAACCCCTGCCAATGGTGTAAATCCCATTGAAGTGTCAATTGATTTACTATCTTGAATGGCTGCTAAACTTGCGCCATTTCCGATATGTAAATTAACAAAATTAACTTTATCTTTATTTAAAATTTCTTGCAATTTTAATGTAATAAAGTGGTGATTCAAGCCATGGAAACCATATTTTCTAACATTTAATTCATCGGTTATATTTGCATCAATTGCATAAGTATATGCTTCTTTTGGAAGGGTTTTATGAAATGATGTATCAAAGTGGAATGTCATTTTAGCAAATGGTAAAATTTCTTTAAAGGCTTTTACAGCTTCAAGAGCACCAGGATTATGAACTGGTGCTAATTCAATAGCATCTTCTAAATATGCAATAGTTTGAGGGGTAACTTCTGAAGTGTCTTGTAGATGTATTCCACCATTAACAATTCTAAAAGCAACATATTTAATTTCATTAAAATCTGAAATAATATTCTTATTAATTCATAAATTAACTAATTGTTTAACTGTTTCTAAAAAACTAGGTAATTCAATACTTACTTCTTCTTTTTTGTTATTGTAATTTAAAATAATAGAACTATTTTTAATTTTAATTCTTTGTGCAATTCCTTTGGCAATAATATTTAGATCTTTTGAATATAAGGTTCATTTAATTGAACTTGAACCTGCATTAATAATTAAAATTTTTGACATAATAGCTCCTTTATAATTTATAAAATTATATTAAAAAAAATATCTTTTAAACTTAATATGATTAAAAGATATTAAAAAAGATATAAAAAAAAGCAACGTCCTATTTTCCCATTGAGGTATCGTCGGCACTGAAGGGCTTAACTGCTGAGTTCGGAATGGTTTCAGGTGGACCCCTTCGTTATGGTTGCTACGTGTATTATTATATACTATTTTTAAATATGTAATAAAAAATTTTCATTTTTTTTATTACTTTTTAAACACTCTTATTTTTGCAAGACTATTACCAATATTTTTTCAATAATATCCCTCTTTGTCTAATAAGTCTTCCGTTATTTGGCGAATAATTCCACTACCAATGCCTACAATAATATCGAAATAATTATTATATTCATCTTGTTCAAATTCATATAAAGCATTTAAAATTGCTGCTGTTGCGGAATTTACGTCATAGCCATGAAGATCTAACACCATTGGAAAAAAATTGAACTTCATTTAATATACCTTCTGACAATTAGGACAAACATATGTGCCACGTTGGTTAACTTTTAATTTAAGTGTTTTTTGGCCACAAACCTTACATTTTTTTATATTATCACTATGAATATTTAAAAAATCTTGATATTTTCCTTCTTGTTTATTTAATGATTCATAAGAAAATAACGTTGTTCCACCTAGCTCATAACTTCTTTCCATTATTTCATTTGCGTTTTTTATTATGTCTTTAAGTTGCTCTAGTGATAAATTTGAAGCTTTTGAAAGAGGATGAATTTTGGAAGCAAACAATGCTTCATCAGCGTAAATATTTCCTATACCCGCAACAAAACTTTGATCAAGTAATTTTGTTTTAATGGGCATTTTTGAATTTTTTATTTTTTTAAATAATGCTTCAATGTCAATATCCTTTGGTTCAGGTCCAAGCAAAGAAATTGGAGGAATTTTGTTATAATTTTCTAAGTTTCTTAGCATATATGTACCAAATAAACGTTTATCTAAGAAGTGTAGTTCACTGTTGTCGCTAAAAACAAATCTAGCAACTAAGTGCTTATCAATTTCTTTGGGTGGATTTAAATAATAACGATATTTTCCTTCCATTCTTAAATGAGAAAGTAAAACTAATTTTTCAGATAAAAAGAAAATTATATGTTTGCCAATGTTATCTATTTTTTCAATTTTTTTATCTCTTAAAATTGAAATGAAATATTGAGGGCTAAACTCTTTAAAAATTTTGGGTTTGTATATAATCAAATTTGTTATTTTTTTATTTAATATAGTATTATTAAGGGCTTTAACAACAACCCTAACTTCAGGTAATTCAGGCATAGTAAAATTATAATACTTTCGAATACATTACTCATATGATTAACTGCAAAAATAAAAGTGTTATATAATTTAAATTATGATAAATAAATATCAAAAATTAATTATTTCAACTACAGACACAGTTTTAGGAATTGGTGGCCCTGTGTCAAAAGAAGTTGAAGACCTTATTTATGAAATAAAAGGTAGAGACCGCAATAAAAAATTAATAATATTAGTAAGTTCAATAAAACAAGCAAGGCAATTTAAGGAATGAAATTCACAAGCAGATGAATTTGCTAAAAAATATTGACCTGGTGCAACTACCATTGTCGTAAATAATCAAGGTTTTAGAATGCCAAACCAAAAGAAATTGCTTGAATATATCGAAATAAATGGTCCAATTTATATGACAAGTTGTAACTTGTCAAACGCACCTGTATGTAAAACTATTGAAAATGCAAAGCAATTATTCCCTGAAGTTACTAATGTATATAATTTTGGCCCTATGACTCAAAAACCTAGTCAAATAATTAGAGTAGAAGATGGAGAAATTTTAAGAAAATAATATGAAAACGACAGTTAAAATAACAAGCGATCACGCAGGATATGATGCAAAAATAAACCTTGCAAAAAGATTGGAAAATGAAGGCTACAATGTAGAATTATTTGGCTCAAAAACAAATAACAATAGTGATTCTTATGCAGATGCTGCTATTGAATTAGTTAATGAAATTCACAAGGATGGCAATCCTGATGAAAATAAAAATGTAAAATATGTTGCATTTTGTGGATCAGGTATTGGTATTGAAATGACTTTAAACAAATTTAAAGGCATAAGAGCAGCAAGAGTTACAAATGAAAACGAATCTCACCTAGCAAAATTGCATAATAATGCTAACGTACTATGTATGGGTGGAAGATTGTTAGAAGGTAATGAAATTGAAAAAATTTTTCATAATTGAGAAAACACCGAATTTGAAGGTGGCAGACATCTTGAAAGAATTAAAAAACTAAGCTCAATTGGTGAAAACTAATAAATTTATAACAAAAAATAGGCTCATTTTATTGAGTCTATTTTTGTTTTTATATATATTTTATTTTTCAATCTTATAAGTATTAAATCTTCCAGATACGTTTTCTACTTTTGATGAAGTTATTCATGCATTACTATCGGCAGCCAAAATTTCTTTTTTAATCATATTTTGTTCCAAGAACAAACAATATGTTTCAATTTTAACTGTTTCTGTATTGGTATAACCTCCCAACATACGGTCAATAGTGTAAGCGTGTCAATAGTTAATTTCTTTGAATTTGTTTAAAATTAAATCGGGATTCTTGCTGTAAATTTCTAGTTTTACTTTTCTGTATTTTGGATAAATTGATGCCATGATAAATGTATATAAGAATATATATACAAATGTACAAATAATTCTTAATAATAATGCTGCATGGTTTATTGGATTTCCATTTTGAGCATGTGATATTACCCCGGTAAGTTCGCAAATTGAAATAACAACTATTGAGAAAACTGCAAAGCAAATTGCGACTATTAAAGAAATTAAGCCTGGTTCTTTTTGTTTAGTTCTTGAAATGTAGTAGACAACAAAGTCTCCCCCTGCTGTTGAAGCAGAATTTTTTCAAGCAATTCCCCCTGCACATCCTCCGCATAATGCTCCAAGCACTGCAAAAATGATAATTGGTCAAGTAGGATTGTTATAACCATGTATTAAATGTTGATAGAAATATCAATAAGACATTGTTTCTTGGTAGCTTAGTTGCACGCCGTTTAGAACTATTCCATTATTTCAAGAAGGATTAAATCCAGTACTTTTTATAGTATTTCAAACGTTTGAATAATGCGTTAGATAGTTTCCATAGACATCCCATGGTATTAATACATTTAAAGAGTTAGCAACTTCGCCATGTGCTGGAGCTAATGTTGCAGAATGAGGAGCATACCAGTTTACATAATAAAACGTTATTTGACTAAAGAAGTGGCCAACTTGTGGAATTAACAATATTGATTGAAAGATAACTTGTCAAAGTAACCAATAAGTTGTTAATATCATAAATAGTCTAGAATTTTTCTTTCAAAATGCAATTACAAAAGGTAAATTCATTGCTATATAAATATAAGCAAAATATGGTGCAGTTTGAGTTAATGTTAGAGTAATGGCTTGAACCAATGCAGATAACCCGGTTGCAACAGTGGCAGCCTTAGCCAAGAATGTTGCAACACCAATATTGTATAAAACGGCTGAAATAATAATCATGACCATTTTTTTAGGAAATTTCTTTCAAATATTAAAGAAATTTACCCCATAAGGATTTATGTCAATTTTGCCAACTGATCCCTTTTCTTTTTTGGTTTCTTTCTTTAAACGTTTACGTTCTTTAATCTCAGCAAGTTCCTCAGCTGTATGTTTCATTTTCACCTCCTTTTTATTATCGTGTATATTCTAACACTTTTTAAGTGTTTATATTTTTTATTAATTATTTTTTTTAATAAAAAATACTTTTATATGACTTTAATAATTAATCTACGTATGAGGTATCAAATGAACCAATTAATCCATGAATATTTGAGATACTTATTCAGGCTTTTTTATCAACACTTCTAACTTCTTCTAAAATTGATTCTTTTTCTAAATATAGGGCAATTGTTTCAATTTTTCCAAAATCTTGATGGTTATATCCACCAAATCCGTTAAATACATTAAATGAGTGAACATATCCAATTTGTTTGAATCTTTCAGCAACTAGTTCTGGTTTTGTGGTGTAAATATCAATTCTTACTTTCTTGTATTTTGGATAGAACTTATTTACTAACATTGAAAATACTAATAAATAAATTGCAGTACCCATAATTCTTACTAAAAATTCTGAAAAATCTCATGTATGTCCAATAATGTTTCTTGTATATTCTAATGAACCTATTACAATTGTTGAACATGATGAAAAGCAAATTGCAACCATTAAAAATACTGTTCCTACTGGTTTTTTATTTTTATATGCAATGTAATTAGAAATAACAGATGTACCGCCAATTGAACCACGTTGTTTTCAAGCAACTATTGATGCAAGGCCTTCCATTATTCCCCCAAGAATTGCATATATCAAAATAGGTCAATTTTGGCCATTATATATTCCATAAATTTTAGTATTTTGACCGCCTTTTAAATCAATACCTGTTAATAAACTAGGGATATTAGCAAGCGATAAAGAATTAGTTTTTTCAAAAATTAATTTTGCGTTATCAAATACCATTTTTAAATCGTTAGTTTTAAATAAGTTGGCTAGATACTTGGCAATAGCTAAATTATCTCTTGACAATGAACTGCCAGTTCAAGCTGTTTTAGTTGATATACTTAAAGCGTAGTTAAAAATATCTTGATAATTTTCTTTATTAATATAATTTAATACTCTTGTATCAAAACCATATGGATCTCAATAAAATTTGTATGTTAATCCGTTATTTGTAAAAGTTTGTCCTATTGGATCATAGAGATTAATTCTTTGATTAATAAAGTTAACAATAGGATTTGATGCTCTTCCTTGATATTCAATAAATATACATTGTACAATTAATTGGAAAAACATTCAATAATAAGTTATTACCATAAACGTTCTAGGATTTAACCGTCAATAAATAAACATTAAAGGTAAGTTAACTAATACATAAAATAAACCATAAAATTGTGCAGTTGCAGGCGCTGTAAAAGTAATTATTTGAGATAAAGATGAAGTTCCTGAAGCTATTGTTGCTGCTTTTTTTAAAAAGACTGTAATTGCAATATTATATAAAAATGCACTTACAAGCATTCAAAACAACTTTTTAGGATATTTTTTTCAGACATTTATTAAATTTATTTTTTGTGGATTTAGAATTTTCATTTTTTCTAAATACCGTTGGTGTTTTTGCTCTTTAGTAAGAATTATTTTGTTGATTTTAGTTATTTTTTTATCTTCTAAACTCATGTTGCCCCTTTCTTTAGATTCATTTAAACTATTAAATTATTAATTTCTCTTTTTTTCATAAATAAACCTGTTGCATTCTGGTTGGAATAATAGTTGTACAATTCCAATAGCCCCATGTCTATTTTTTGCTACAATGACATTGGTTAAAGAAGAACTGCCATTGACAATTTCACTTTTGTCAACAGCTTCTGCTTTATCATTGTCATCATTAATGTTGTGCTTATTATAATAATCTTTTCTATGTAAAAAAATAACCATATCGGCATCTTGTTCAATTGTTCCTGATTCTCTTAAATCTGACAAAATAGGAGTTTTATCTTCCCGTTTTTCAACATTTCTACTTAATTGGCTTAATGCTAAAACAGGACATTCTAATTCTCTAGCAAGTTGCTTTAAGGATCTTGAAATCTTACTTACTTCCAATTGACGATTTTCCATACTTGCTTTTGAAGAATCTGACATTAGTTGTAGATAATCAACTACAACTAGATCAACTTTATTGTTTCTAGCAAATCGTTTTGCAATGGTTGTTATATCCGAAATTGTTGATGTAGGTTTATCATTTAAATACATTTTTCATTGTTTCATTTCTGAAACAGTTCAGGCTAGATTTTTAGCATTTTCTGTTGAGATTTCATTTTTTCTCATTAAACTAATTGGAACCAAAGAATCAATTGACATCATTCTTTGAACAATTTGGGAATTTGACATTTCAAGAGAAAAAAACAGCACCGTTTTATTGTTTTTGGCAACATTATTTGCTATATTTAAAGCAAAAGCAGTTTTTCCCATAGCTGGGCGAGCTGCCAAAATTATCAAATCTCCGGGATTAGCACCTAAAATTAATTCATCCAATGGAGGAAATCCAAAAGAAAGGCCAACGCCAATATCTGATTTTTCTTTGCTCATTATTCTATTAAATAGTTCATCTGCTTTATTTCCTACTTGTTCATAAGCAGTATTTATTTCTGAAATATCTAGATTAATTAAATTCAATTGTAAATTGGATAATAAATCTGAAATTTCATAAGGTTTGTTTTCCATAATATTTATTGAACTATTCAATAAATCTCGTAAGCGATCCTTCTTGTATTGATCAATTATTTTTTTGGCATACGACTCTAGTTCAGCACTAAAACCTGCGTTTTTAGTAAGCCAACTAATATATTCACTACCACTATAGCCAAATGAACCAATATGATCAATTAATTTTTGGTCATTTAAATAACTCAATAAGTTGGCAATATCAAATTGTTTAGAGGCAATATGAACTTCTGAAATAGATTTAAAAAGAATTTGATTATTTTTAAAATGAAACATTTCAGGTTTTAAAATTTCAGCAATTGTTAAATAACAATTTGGTTCCCTAATTAATAAACCTAATAACGATGTTTCATTATTAATAATTGCTAATTCTCTTTCTTCTTGAATGTTCATTTTAGCCCCCTCATATTTATTTTAATTATTTCTCTTCTATGATATTTATTTTTAGTGTTGCAGTTACTTGGTCATGTAATTTAATTTTTACATCGTGCAATCCAAAAGTATTATATGTTACCCCTGCTTCAAATGCATGGCTTGGGATTTTAATGTTGTTTTTTATTAATTCTTTTTCAATTGCTTTTGTTGTAACATTTCCGTGCACTATATTTCCATTAGATTTTAAATAGAATTTTAAAACTAATGATTCAATTAATTGTTTGATTTCATTAGCTTTAGCTATTTGATTTGCTAAGTCTTCTGCAATATTTGATTTAACTCTATCTAAATTTGCCAATGTTTGTTTGTTAATTGGTTGAGCATAACCGTTTTTAATTAAAAAGTTTTTTGCAAAACCGTCTGTAACTTCAATGATTTCATTTTTTTTATGTTTTTGGTATTCTTTAATTAATATTACTTTCATTATCTTTCACACTCACTATTGCTTGTATTATATTATCAACAAATAAATTCAAATTTTCAGTTGTTTCAGCAGCGGCTGCCCCAAAATGGCCACCGCCGTTAACTGCTTCTGCTATAATTTGAACATTTGTATTAATTCCTCTTGCTGACATTTTGTATGTTTTTGTTCCTGGTAATTTAGCAACAACAAATGCAGCTTTCCTACCATTAACTCTTAATATTTCATCTGACGCTATTGAAATAATATCTGTTGATGCTTCAATATCTTTGTATGCCAAGAAATATCCTTTTTTAATTTCTTGCAAATTGGCTAATAATTCTTGAACAATCTTAAATACTTTTTCATCGAATTTTAATGTATCAACAGATTTATCATTTTGTGCTCCTCATTTTTGCAATATTGATGCTGCTGCAAAAGTTTTAGCACTTGTTTGTTTTTTAAATGTATTAGTATCTAAATAAATTCCATCTAATAAATATTGCGCTGTATCATTATTAATTTTATCTTCTTCGTGTAACAATGTGATGATTTCTGTTAATATTTCAGAAGCAGATGACGCCGAGGAATCAATATATACATTTTCTCTATATGCTATTTCATCAGTATTTTTATTTATTCTATGATGATCGATTATCATGATATTTTCTTTTTTTATGTTTTTAAAGGCATTAGCATTTTCAATTCTAGTATCATCTGAAGTATCGCAAATTACAACCAACGTTTTTTCGTTGTTGATGCTTTCTGCTTCTTTAGGACTAATAAAATATTCTTGAGTTTCCTTGGACATTTTATCAATAACTCTTTGAGTAGTACTATCAAATGTCTTGTTTTGAATATAAGCATTCTTTTTATAGGCTAATGCTAATTGCAAAATTGCATATGTTGAACCCAAGGCATCAAGGTCTGCATTTTTATGGCCATAGGCTATAACATTTTCAATATCTTTTGATTTTATCTTTTCTAATAAGTTTTGCGCTATATAGTTTACATTTGTCCTTGATAGATTTACATCGATTTCCGAGCTTGAGCCATAGTGTCTAGCTTTTTCATTTTTTGTTAAAACTGTAATCTGATCGCCACCCCGCATTTGAGATTGCATTAGAGCATCTTTTGCTAATTTGACTAACGTATCGTATTTAAAAATACCATAACCAAATCCACCAGAAATTGATATCAAAACATCTTTAACAATTATTTTGTTTTTTACTGAAGTTATAACATTAAAACTATCTCGTTCTAATTCTTCTAATGTTTCTTTATTTGTTATTAAAAAGAATCTTCCGTTTTCATATTGTCTATAAATAACATTGTATTTTTTAGATATTTCATCTAATAAATTTACTACAGAAGCATAAATTTTAAAAATTTCTTCTTGGGATAAAAATGTTTGATATAAATTAATATTGTCAATGTCTAATTCCCCAAACACAATTCTTTGTTTTTTATAGTCATCTAATAAATTTTGTTGGGCAGTAACATCTCTTAAAATAACTAGATTTTTTTCAAATGATATATGAACTTCGTAATTGCATCCATCTATTTCATACATGAATTCTTGATTATCCGAAGTTCATTGTTTTATATTAAAGATATCTTTAATGTTTTTGTTGATTAAATTGTTATGAAATTTTATTTTTAAAGATTCTGATGCTCATATAATTTTTCCACTATTTAAAAATATCAAAATTCCCGTACCAAATTGATTAAATTCGCTTTGTAAATAAAATTCCAATGATTTTTTAGCTAATATTGAGTTTTTAAAATGACGTTGCAAAATAATAGTTAAATAAATTGCAAGGCCGATTAAAAATAAAGTATAAATTGCCATTAACGCTATTTTTGACTGTTGAGTCTTTAAACAAAAAATGCCAATAAAGAATAATATTGTTGGCAAAATAAATAATAGGATAACTTGTAAAGCTATATAAATTTTTTGAATATCTTTGTTTTTCATTTTATATATATAAAATTATAATAGAAAGTTAACAATAGATTCAATTAATGATACTACAAGTCATAAAATTGCATTATTTCTTCAATACCTTGTTTTTTTTCAGATGATACTAAAAAAATATTTTCAGCTGTTTTTAAAGACAAGGCTTGATTTTTAACGCAATAACGTTGAGATTGATTTGCCTTGTCGATTTTTGTAACAACTATATCAAATTTTATTTTAAGATCGTTAAGATATTCTATTATTTCTAAATCACTAGCGCTAAAACCAATTTTAGCATCAATTAATAGCAACACTTTTTTATTGTTTTTATCGTTTTTAAAATAAAAGTCAATCATAAAGGAAATTTTATCTTGCAATTTCTTTGAAATTTTTGCATAACCATAGCCTGGTAAATCAATAATTATTTTATTTTTGTTGCTTTCAAAGTAATTAATTAATTGTGTTCTTCCTGGATAAGATGATGTTTTTGCAATATTTCAAGAAGCCAGTGAATTAATTAATGAAGATTTTCCAACATTAGATCTTCCTCAAAATACATATTGTATATTGTCAGAATTAATAAAATCATCGTGGCTCTTACAACTTTTTAAGAATTTTCACATTATCTATCTCTCCTTAATAGTTCATATGCTAACCTAGCAATCATCATTGCATTATCAGTTGTATATTTCATTTCCGGAATTATGGCATTTGAATGGACATTTAAAAACATTTCTCTAATTGCTTTATTAGCACTTACTCCGCCTGCCAAAACAATTACTCTTGGATGATATTTTTTAATTGCAATTTCTAAATGATTTTTTAAATATAAGACTACTGTATTTTCAAACATTGTTGCATATTGATCAACATTGATCTCTTGTTTTCTATTGATTTGGTTATTTATAAGATTTATAACTTGTGTTTTTAATCCACTAAATGAAAAGTTCAATTGAGCTTCAGTATGTGGAATAGTAAAATGTTCTGTGTATTTATCTCTAAATTCCATTCATTTTTTATCAATAATAGGTCCTCCAGGAAAACCCAAATTTAATTTTCTTGCTATTTTATCAAATACTTCTCCGACGGCATCATCTTGAGTTTGACCTATAACTTCGTATTCATTTTTTGATTTATATAACATTAATTGTGAATGCCCACCTGAAACAATTAAACCTAAAGCAGGGTATTCAACTTCTTTGCCTATAAAAGCAGAGAAAAAATGCCCTTCTAAATGATTTAAAGGCAATATAGGTTTTTCAAGTAACATTGCTATTGCATTAGCAACAACATATCCGACATGTAAAGAACCAACTAATCCTGGTTCTTTTGTATATGCTATATAGTCAATCTTTTTAATATCAATTATTTTTTTTAATTTTTCAATTAAAATACCTATATTTTTTACATGCAATCTTGATGCTAATTCTGGAACTGTGCCTCCATATTTTCTATGAATGTCTATTTGGCTAATTGTTTCCATTCAAATTGGTTTGTTATCTTCTAATAAAGCAAATGATGTATCATCATGAGAACTTTCTATGGCAAAAATTAACATTTTATCTCTCCTATTTGTGGATCATGCAAATAAATTAATTCGCAATTTTCAATATCATTTACTTGTTTAAATTCTTTTAAATATTGCTCTATATTTTTTGAAAAAAGCTCTTTATTGAATAAATCAAAATTGCCTAAGTTTTCTTGCATATGACATTCAAATGGATCAACAATTATTGAATAAACATTGTATTTATTGGCTTTTATATTTATTAAATTGCAATCTTCATGTTGCAATTTTTTTTGTGTATAAAATAAAGAATATGTTGGGGCTACAAATAATTTGATATTTGATAATTGTGCTACTGTTTTGGCAAAAACTAAGCCAATTCTTGCACCTGAAAAACTCCCAGGTCCTAACGTTGTATAAATTTCTGAAATTGAACTTAATTTAAAATTATTTTTATTTAATAAATAATTTAAATTTTCAAAAAATGCATCGGTTTTTTTAACTAAGTTAGCAATAAAAATACAATCAATCTCGCAATAATTTTCATCAATTAAGCTTATATAAAAATCGCTTAATGAAGTTTCAATGAATAATTTCACTATTGTTCCTCCATAATTTCAAATACATGGTTTTGTTCATTGTCAAAATAAACTTTTATTTTTATATAGTGTTTAAATTTGGGCAATGTCACATTTTCTGCTCATTCAATAATTGCTAATTTATCTTCAAAATAATCTTCATAAGCAAATAAATTTGTAGTTAATTTATAAGCATCAATATGTACAAATTTATCATACACTAAAATTGTATTGAATGTGGGTGATATTACAGTCTTTGGTTCGTTTAATTTTTTTGCTATTTGACTTGTTAAAGTTGTTTTGCCTGCTCCAAGTTCGCCTATTAAAAGCAAGGCTTCTAAATTTTTAAATGACAAAATATAGTCAACTAGTTTATCTAATGGTTCGTTATTTTTATAAATAAATTTTAGATTTGACATGATTTCTTAACCTTGATAATAAAATACATTATTATTTTACCATTTATGCACCTTTTTAAGGCTTGTTACAAATAAAAAATGTATTTAAAATTTAAAAAATAAACATTAAGTATTTAAAACACAAACTTAATGTTTATTTATTTTTTTAAGCAAGATGTTATTTTATTTATATTGAATATTTTCTCAATCAGATTGTTTAAAATCAGTTAAAAATTTTGTTAACAAATCAACGGCTGCCAATAAATCACTTATGCAACATACTCCAATTGGTGAATGCAAATATCTTTGAGGTAATGAAATTGTTAATGTGGCTGCCCCACCTTTTGAAAATTGTAATTCAAAGGCATCAGTACCACCACCCATGGCTACATATTTAAATGTTGGAATATTATATTTTTTAGATAAATCAACAAAAAATTGTACCAATTTTGGATCCATCATTGTTCCTCTATCTTTTAATCTTAAAGCAGCTCCCTTATATAAAGAAACATTTCCAGGAATTGTATTAGGTGTATCGTGAGATGCAGAAGTATCAACTGCTATTGCAATGTCTGGATCAATCATAGATGCAACTGTTTTAGCTCCTCTTGTTCCAACTTCTTCTTGAACTGTACCTACTAAATATAGATCAATGTCAAGATCTAATTTTGCAACAGCTTTAGCAACAAATTCTAACACGGTTACACCAGCACGGTTGTCCATTGCTTTTCCACCAATTAAATTTTGATCATTAAATAGTATTGTTTCTCCGCTCATGTAAACTCTATCGCCAACATTAACACCATGAGCAATAGCATCTTCATCGCTTTTGAAACAAAAATCAGCAAATAATTCATTTTCTGTAATTGCTTTCTTTCTTTCTTCTTCTTGCATTATATGAATACTTGTATGTCCAAATAATCCAATAAATGTTTCGTTATTTGAAGTAATTAATTTTGCTTTGGTTCCAATAACTGCACATGGTCATACACCACCAACTGTTGATAAATATAATTGTCCTTTTTTATCAAAATTTCTTACTACATATCCAACTTCATCCATGTGTGCTGCTAATAAAACTTTAGGAGCATTTGGATTTTTAGATGGCTTGTGCATAATTAAACAGCCAAAATTATCTCTGCTAAATTCAAATCCTTCATTAGCTAATTCTTTTTTTAATTCATTAACAACTGGTTCTTCATAACGAGACATTGCTTCAATTTCCATATATTTTATTAATCTTTCTTTGAATTGATTTTCTCTTGTCATATTAATTCCTCCTTTATTTAATGTAAATGTATAAGATATATTTTATAACAAATTGTATAAAAAAATAGCCATTAGGCTATTTGTTGTTCTTTGAAAACTGAATACGACTTAATTTAGATAACTATATAGAATATTACTATTAAACCAATAAATTTATTAGTACTGGTCAGCTGAATGCATTGCTGCACTTACACCTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACACCATCGGTGCGTCCACTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGATCTCTTGGGAAAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCACCACACACTGTCCTCTTCCCGGATGACGGGAACAAGTTAGAAGTTCAATGTAACAAGGGTGGTATTTCAACGGCGACTACTCTTAAACTAGCGTTCAAGCATCAACGTCTCCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAGTCTATTGTCGAGACAGTTAAGAGATAATTACTCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCACATTAATGCTTCGCTAATGCTAACACCTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAACTTACGGGATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTGTGTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAAATCATCTAATTCGCTACTCAGTTGCCCTTTTGCTATGCGTCGTAACTCCCAGTTAAGTTATGCGGATTTGCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCCTTTCGGCCTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAAGCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAGAAACTTCACAACCTTTCTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTATCACCGATGTACCGACTGCATAGTAATACATGATAGTATTCGGAGTTTGATTATAGTCAGTACGGCTAGGCGCCGCCATTCCATATTCAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCACCCCTACCCACAAGTCATCCGGGCACTTTTTAGCGTACTGCGGTTCGGTCCTCCACTTAGTGTTACCTAAGTTTCAACCTGCTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTGTAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTCACGTGCCCCGCCCTACTCAGGATACTGCTAGGAGATTTATACATTTCGCTTACGGGAATTTCACCCTCTATGTTTAAGCGTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTCTTTATTTTCTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTCCAGACCCAAGGCATCCACCAAAAACTCTTGCTTGTTTAATAGTTTTGTTAATTGTATGACCTATTGTTTGTCTTTATAAAATATAAATATTCTATGGTTGATCTAAACTTTGAAATATTAATTAATATATCAATGTCGATATTCAGTTTTCAAAGAACCGAGAGATAAATCTCTCAAAACTGGATACAAATATTTTTTTCCATAACGTAGGTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTCACCCCAGTCACCAGTCCTACCTTAGGCGGTCGCCTCCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCAGTTACCCAGGCAGTATCTCTAGAGTCCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGACTTGACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCACTCCGATAACCTCCACTATATCTCTATAGCTTTGCGAAGGATGTCAAGAGTGGGTAAGGTTTTCCGTGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGCCCCGGGATTTAACTCCAGACTTAACAAACAGCCTACGAACGCTTTACGCCCAATAATTCCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCATCGCTTTCTGACAAGGTACCGTCAGTCTGCAATCATTTCCTATTGCAAATGTTCTTCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGTCTCTCGACCCGGCTAAACATCATAGTCTTGGTGGGCCATTACCTCACCAACTAACTAATGTTCCGCACCCTCATCTTTTAGTGGCGCCTTGCAGCGCCTTTCACAACGGAACCATGCGGTTCCATGCGTATCCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATTAAGCACGTGTTACTCACCCATTCGCCGCTAGGTTATTGCTAACCTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTTATAAAAAATTTGAATTGGTTGATTATAAATAAAAAAATAAATTGACGTTAATGGTATTCGTATCCAGTTTTCAAAGAACTATCTCGTTGTTTCAAAACAACCTTTAAATATTATATACACTTTTTTTTATTTTTTCAAAAAAAATTAAATTTTTTAGCGATATAAAATTGCTGTTTTAAAACAGAAAAGTAAATATGTATATATTATAACTGTTTTATTTTTTAAATAATAAAAAAATCAGGATTTTTTCCTGATTCTAATATCTATTCATTATTTTATTATTAACCTACTATTTCTAGATCTGCATAATCAAATTCAGTTGGAACTTTTCTACCAAATTGTTCAATATTTACAAAGGCTTTTTGGGTAATATCATTATTTTTAATTATTTCGCCAACTTCATCTTTGAATATTCCACTAGAAATTTTTACTAAAGCCCCTTCTTTAAAAGCAGTTTCAATGTCCCCTGCTTTAAATTTAGCTAGCAACATTGCTTCTTTATCAATCATTAATTGAAATTTTTTATCACTTATTGGAGTAGGCTTTGTTTTGTTACCACTTGAACCAATCAAACCTGTTACATATTGAGTATTTCTAACAAGATATCATGTATCATCAGTCATTGCCATATTTAAAAAAATATAACCTTTGTACATATTTACAAATTTAACAGTAAATTCTTCGTTTCTTTGCTTCTTTTCTAATTCTTTATTTGATAGATGGGGTTCTTTAAACATCCTTATATCTTTAAAGTATTCATCCATTCTTTGAGCAGCAATTTTATTTTTTAATGATTCTATAACATTTTCTTCTTTGCCTGAAACTGTTGAAATCATATATCATTTAAAATCATTATTTGTTAATTCCATATTCTTTCCTTACTAAGCCTTTATTCCTACTGAATTTCATAAACTACTAAATCCAAGGGTTATTGCAAAACAAAATGCAGCCATTAAAACTAGAAATAAAATTGTAATACCAAATCATTTTCAAGATTTTGCAGATTTTGGCCAGATAACTCTCTTTATTTCCTTTATTCAATTTCGAAATGTATATAGCTTAACTTGGCCTTTTTCTGCTTTTGCTTGCAATTTAGCTTGTTTTCTTTGTTCTCTAGTCATTATTTTTCCTCTTTGTGCAATGTTTGTTGATTACAAAATTTACAAAATTTGTTAATCTCTAGTCTCTTTTCATTGCTTTTATTTACCGAGTAATTTTTATGTAAACATACTTGGCAGGCTAATGTAACCTTCTTTGACTTTGTATTCATAATTAGATATTATAATATATTAATTTCAAATTTTCAATCATTTTTAATAGTAATTTATATTAAATAATTGTTGCGTAAGAAATTTTATATAATTTTAAAATATGAGTAAATGAGCAATTTTGTTTTTAGTAATCTTAATTATGCTAATTGCAATGTATTTAACATTTATTTTTTATTATTTTATAAAAATATATCCCCAAGTTTTAAAATCAAAATTGGATAATTTTATTAATAAAATTTGAACATTTATTAATAAAGCAAAAAATTCTTATGACAAAATTTGCCAATTAAGTTTGAACAATGATGAATGTAAGGAACTAGAAAAACAAATTGGAATTAATTTACATGAGGTTTGCAATTTATACAAGGCATGTAAAATATTAAAAACCAAAGTAGATGATATTGTTTATTTGCACATGAATAAGCATACTTTTTGGCATTTAAAATGCACAATTCTTATTAAAAAGAAATATAAAGAAATTTATTTTAAAAGACTAGAAATTTTTAATTTACTTGAAACCATCTTTAAAAAAGTGGCAGAAATTGATAAAAAAAGTGGAAATATACAAAAAATCGTAAATAAATACAAAATTAGTTTAAATGATTTATCAAATATAATGGCAGGTGAACATGAACATTTTCCTCAACACTTTATTGATAATTTAAAAGACAAAAAAACAATTATACAAAAAAAATTAGTTGATTTTAATAACGATGAATATCTAAAATGAAACGAAAAAATATTAAAACAAAATTTATTAGAAATTAAAAATTTAATCATTGAATATGCAACCTTTTATTTTAAATTCAAAAACAATTACAAGAATTTAAATTATATTGCTGAAAAATACAAAAAAAATCAACAGCTTATTAAAGAATTAAATAATATTAAACTAGAACAATTAATAAAAGAAATGTTGGAAAAAGAATTTAAATTTAATTATGAGATAGAAGTTGAAAAAATAAAAACTTTGATTAACAAATGTCAAAATTCAAAAGATATTTCAAATATTGAACAAATCATTAATAAACTATTAGCTTTGATAAATACAACTAGTATAAAAATATATAACTATATTAAAACCTTTGCTTTTCATTTAAAAATACAAAATAATATAAAAAAAATCATTGAAAAAATTGATGTTCAAAATAAAAAAATTTCTGATTTCTTTGCAAGTGAAACTAAAAAGAATTTATTAAAAAGTGAATACCTTAATACTTATAAGCCAATATTGAATAGCTTTATAGATATTAAAAATAATAATTGAATGGATATAGTTGAATTTATAGAGTTGAAGAGATGGTTAGAATCGTTATTGAATTTGTTGAAGGTTCAAAATAAAATAATAAATAAAATAGACGAACAAGAATATCAAAACAATCAAATTGAATTTTATATTGAAAACTTTAAAAGATTAACTGCCAATATAAAAGTATTAAACATTAGTTTAAATCCAATAGACATTGAAAACATAAATAGTTTATTTACTTTTATTTATAAATTCAAAAATAATAATTTTTCTGCAAACGAATTGAATGACTTTTTAAGTTATTTATCTAATGAAATAAATTTTTTAAATTGCAAAATTCAATATTTTTTAATAATTAAAAATGTCGAATTTTCTTTAAGAAAAGACGTAAGTGAAAATTCAAACGCCCAAAATTTGTTTAATGAGTTGCAAAAACTTAAAGGTTATAATGATGATAAAAAAGCAATTGATTTAATATATGATTATATAGAAGAACAATACAACTAATGGAGTAAAAATGGACTACAATAAAATTTTAATTAGATATGGTGAATTAACTCTTAAAGGAGAAAATAAAAAGGATTTTATTAGGCAATTGCATAACAACCTATCTTGATATATAGAAAACTCAGAAATTAAAACCGAATATGATCGAGCATTTGTTGATTATTCGCAAAAAAATTTAGACTGCTTAAAATATATTTTTGGAATTTCTTCATATTCGCCTGTTTATGAAACAGAAACCAGCCTAATTAATATTGAAAATATAATAAAAGAAATAATTAGTAAAAAACAATTTAATAGTTTTGCAATAAATGCACGAAGACACAACAAGAATTTTTCAATGACTTCAAGTGAACTAAATATGTATTTTGGAGGGGTTGTTTTAAAACAAATGCCAGATAAAGAAGTTAAATTAAAAAATCCTGATTTAACTATATATATTGAAATAAGAGATCAACGTAGCTTTGTTTTTTGCGATTATTTTGATGGCTTAGGTGGTATGCCGGTATTGAGTTCTGGAAAATGCATACATCTAATTAGTGGGGGCATTGACTCGCCTGTAGCAGCGAACTTATTACAAAAAAGAGGTTTAAAAATAGTGTTTTTAAATTTTATTACCCCTCCTCAAACAGATGAAAAAACAATTGCAAAAATAGATGAAATTGTTAATATATTAAAAAAATATCAAGGCAATGCTATGTTATATCAAATTAACTATAGCAAAGTAATGGAATATATTGGCCTTATAAGTAATCAAAAATATAAAATAACTTTAATGAGAAGATCTTTTTATAGAATAGCAAATCTTATTGCAAAAAAAGAAAACATTAAAGCAATTTCAAATGGCGAAAATTTAGCCCAAGTTGCATCTCAAACTCTTGAATCTATTTATACTATCTCCGAAGTTTGTGATTTGCCTATTTTTAGACCTTTAATTTCTTTTGACAAAAATGAAACTATTGCTATAGCAAAAAAAATTAACACATTAGATGTTTCAATAGTAAGGGCGTGTGAAACATGTGAATTATTTGCTCCTAAAAATCCAGTAACCAAGCCTAATAGGCAGGAAGCTCATAAACTCGAAGAAGAAGAATTACCTTTAATAAAAGAATTTGAAGAAGAATGTATTAAAAATGCTATTATAAAAAAATATTCAATATAATCGTTTTAAATGTTTAATAATAAAAAAGGTTTGATTGAACAAACCTTTTTTATTATTTTAAATCTTTTAATTGAGGGAATAAAACTACATCTCTAATTGAATCATTTTGAGTTAATAGCATAATTAAACGATCAATTCCTATACCAGAACCGCCTGTTGGAGGCATTCCATATTCCAAAGCATTAACAAAATCTCAATCAATTTCATTTGCTTCTTCATTACCATTTTCTTTTTCCAACAATTGGTTTTCAAAACGTTGCAATTGGTCAATTGGATCATTTAATTCAGTAAACATATTTGCAAATTCTTTTGTTCCAATAAATAGTTCAGCACGTAGTGTAAATCTTTTATCGTCAGGATCAATTGCTGCAAGAGGAGAAATTTCAATTGGGTGGCCATATACAAATGTTGGTTCTATTAATGTTTCTTCAACATATTTTTCAAATAATTCATTGATAATGTGACCATATCTAAAGAATTTTTCAACTTTAATACCATGTTTTTTAGCTAATTCAAACATTTGTTCTTCATTTAATTTTCTAATGTCAACATTAATCTTTTCAGATATAGCATCAATCATATTAACTCTCTTAAATGGAAAATTAAATGTTATATCAAAGCCATTAAATTTAACTTCTTTAATGTTGAATTTATTTATTAAATGTTTGAAAACGCCTTCAGTTCTTTCCATCATTCCTCATAAATCTGAATACGCTTCATAAAATTCAATTGAGGTAAATTCTGGATTATGAGTTGTATCTACGCCTTCATTTCTAAATATTCTGCCGATTTCATAAACTCTATCCATTCCACCAACAATCAATTTTTTTAATGGCAATTCAGTTGCAATTCTTAAATAAAAGTTTCTATTAAGTGCATTATAGAAAGTAATAAAAGGTTTAGCAGCAGCACCACCTAATATTGGATTTAAAATTGGTGTATCAACTTCAATGTAGTTCAAACTATCAAAATATTCTCTAATGCCTTTGATAATTTGGGTTCTCATAACAAATGTATTTCTTGTTTTTTCATTCATTATTAAATCTACATAGCGTTGACGACGTCTTTCTTCTGGATCAACTAAACCATGAAATTTATCAGGTAAGGGTTTCAATGATTTTGTTAATAGAACTATGTCTTGAGCTTTGATAACTAAAGCATCAGTATTAGTTTTTGACAATGTTCCTCTTACACTTAAAATATCGCCAAAATCCATTAAAGAAAAGGTTTTTGCAGAAGACTCATTCAATGTTTTTTTGTTTGCATAAATTTGCATTTGTCCACAATTATCTTGTAAAACAATAAATGGACCACGAATAGTCATAATCCTTCCATTAAATACAAATTCTAGATTTTGTTTGTCAAGATCTTCACGACTTAAATTTTTATATTTTTCATTTAACTCTTTTGAATTTGCTGTTGGTTTTATAGTTTTGTGAAATGCTTGTAAATTGTTTTTTTCTAATTCAGCAATTTTATTACGTCTAATTTCTTCTTGTTCACTAAGTTTTCTTTCCATAAATATTTATCACTTTCTATTTCTTAAAATATCAAAAAAATTATATATTATATGCTTTATTTTTTTTATAATTATTAATGCATAATTGCGTGGTCGCATAGCTCAGTGGAAGAGCACGAGCCTCCTAAGCCCGGGGTCGCAGGTTCAACTCCTGTTGCGATCGCCATTTTTTTATACATTTTAATTAATTAAAAATAGTCCAAAAGGACTATTGGGCATTCTTCATAGAACGCATAACTTGTCTAATTTGTGCTTCTGAAGCTTTTCTCCCCATTTGTTGAAACATTACACGAATCATTTTTTCAGTAACTGGTGGATTTTCTTTTAATTGCTTCTTGAATTTTTTTTGTGTTATGTATACCGCAATTATTCCACCGATTATGGCAGTAATTATTGGTAATATAACTAATAATGCTATTCCTCAACCTTGCATATTCTACTTTCCTTTCAATACTGTCTTCTTTAAAATAATATATATTATAAAAGGCAATACAATTAAAACTGCGATACTTATAAAGAAAATTTTGGCATAAAATTTGTGAGCTTTCCTTTTGCTTTCATCAAGTTCAACAAAATTCATTCCTTGCTCATTGCCAAAGAATGATTGGATGTCCACCATTCTCTTCTTTTTAAAAATTGTACTGAATATAAATAATATTAAAACTAGTCCAATTATACCACATAGTACGTAAATTAATATTTCGGGTTTAGGAAAAGTACTATGAATTCATTTTTGTCAATCCAATAAACCCCATTTTGCGTCTTTTAAACCTCAAAAAATACCTATAAACACGCCAACATAAAACACAATAGCAACAACTGCTCATGTTTGTCTTACTTGTCTTATCATTAATTTCTTATAAAGTAGGGCAATAAATGGCGGTGTTGATGTCGCACCATTTTTAATGGTAATTCTATACATATCTATGCTTTTATGTATTCCTGAAAGTTCAATCAATTCAATAGTAAATCTAGTTAACGAAATTACTATAAATATTGCTGGAATAATATATCAAGCCCATTTTATTTTAAAATCTTTTGCATTAGAAAAATTAAATAAATTTGGTAAAAAGTAAAGCAATAATATTGCTATAACACTCAAAAAAAGGATACTAAAATTTCAAAGTATCAATATTTTTTTGATATTCATTTCTCTTTGAAAGACTGAATAAATTCCATTAGGAATTATTTTCTGAGGATCTTTTATTTTGTAACTTATTTGTTGCAAATTTTGAGTATTCATTGAATCTTCTACCATGTGATTTTCAAAGGTACGTGTTGGAATTTCAATGTTTTTACTACTATCGTTATTACTTCCTGTTGTTGTGGGCTTAATATCCATTATTTTCTAATCCTTTCGAGAATCATCTGTTTTTAATATTGCCAAAAATGCTTCTTGTGGGACTTCAACGCTTCCTATTTGTTTCATCCTTTTTTTACCTATTTTTTGTTTTTTTAATAGTTTTTGTCTTCTTGTAACATCACCACCATATAACTTAGCTGTAACATCTTTTCTATATGCCTTAATTGTTTCTCTTGCTATAATTTTAGCACCAATTGCAGCTTGAACGGGAATTTCAAAATTTTGTCTTGGTACCACTTCCTTTAATTTTTCTGTTAATGCTCTACTTTTATTATATGCCGAGTCTTTATGGACAATCATTGCTAAGGCATCAATTTTCTCACCATTTAATAAAATATCAACCTTTACTAAATCGCTTTCTCTTAAACCAATGTAGTCATAATCAAATGAAGCATAGCCTTTTGAATAGCTCTTCATTAAATCAAAGAAATCAAATATTATTTCATTTAAAGGCAATTCATAAATCAATAATCTGCGAGTTTCATCTAAATATTCAATATTTACATATTTACCACGCTTAGATTGGCATAATTCCATAATTGGGCCCAAATATTCTTCGGTTAACATTATATTAGCCTTAATATAAGGTTCCTCTATCATTTTTATATAACTTCTGTCTGGAAATAGTGATGGATTTGTAATATATTGAACATCCCCATTAGTTAGTGTTACAACAAACTCAACTGAAGGAGCAGTAGCAATAATATCTAAATTAAATTCTCTTTCGAGTCTTTCTTGAAGAATATCCATATGCAACAAACCTAGAAAACCTATTCTAAATCCAAATCCTAAGGCTTTTGATGTTTCTGGTTGTCATACCAACGATGAATCAGATAAACTAATTTTTTCTAGGGCATCTTTTAAATCATTGTAATCTCTAGTATCGACAGGATAAAATCCAGTATAAACAACTGGTTTTATTTTTTTATATCCTGGCAATGGTTCAGGGCATTGATTTTCAAATAATGTGACAGTATCACCAACTTCAACATCTTTGATATCCCTTATTGATGCTGAAATTCAACCAACTTCGCCAGCTTGCAATTCATTTTTTTGAACTTCATTAGGATTTTTAACGCCTAGTTCCGTTACTAAATAATGCTTTTCTGTTTGCATAAAGTAAATTTCGTCGCCTACTTTTACTTTGCCATCAAACAATCTCACATAGATAACAACGCCACGATAATTGTCAAAATAGCTATCAAAAATTAAAGCTTGCAATGGTTTATTTATATCTGCTTCTTTAGGAGCGGGGATTTTGTTTATAATTGCATCAATTACTTTATCAATATTCAATCCTGTCTTTGCTGAAATCATAATTGCATCAGAAGCATCAATTCCAATCATGTCTTGAATTTCTTTTTTTACTCTTTCGGGATCTGCACTAATTAAATCTACCTTATTAATAATAGGAACAATTTCTAAATTATTTTCCATTGCCATATAAACATTAGCCAATGTTTGTGCTTGAATTCCTTGAGTTGCATCAACTAGCAATAATGCTCCCTCACATGCAGCAAGTGATCTACTAACTTCATAAGTAAAATCAACATGACCGGGTGTATCGATCAGGTGAAAAATGTAATCTTTATATTTAATTTGAATAGCATTTAATTTAATAGTGATTCCACGTTCTTGTTCTAGTTCCATTGAATCTAAAATTTGAGGTTTTGCTTCCCTGGCAGAAATTGTATTGGTAAATTCCAAAATACGATCAGCTAAAGTACTTTTACCGTGATCAATGTGTGCTATTATTGCAAAATTTCTTATTTTTTCGTTTGTCATATTATTTTAAATTATATTTAAAAATGTCATACATTTAAAGAAAAATAAAAAATAACCATAAATTAGATATATTTATTAAATATCTTTATGGTTATTTTTTCTTAATTATTTTTTGTTTCCTTGGCACCAAATATTTGTTCAAATATTTCATCATAATAATGAACTGGTACAAATTCAACAACATTTTTAACTTCAGCAGGAACATCTTTTAGATTTTTATAATTATCATATGGAATAAAAATCTTTTTAATACCAAATTGAGCAGCTGCCAATGATTTTTCTTTTAAACCGCCAATTGGTAGAACTTTTCCACGCAATGTAATTTCTCCAGTCATACCGATATTAGCAGGGACTGGCCTTTTAGATAAGGCACTAATTATTGAGGTTGTGAATGTTACTCCGGCACTTGGGCCATCTTTTGGAATAGCACCTGCTGGAACGTGAATATGAATTGCTGTATTTTCAAAATCAAAGTCAATATTAAATTTGGCAGCATTTGCTCTTACATAAGCTAATGCAATTTGAGCAGATTCACGCATAACATCTTTTAGTTGCCCTGTCATTTTTAGGCCTTCTTTTCCAGGGAATGTTGTAACTTCAATAGCTAATGTTGAACCACCAAAACTTGTATATGCCAAACCATTAACAACACCTATTTGTGCCTTATTTTCATTTTCATCATCATCGAATTTAATTGGTCCTAAAAATTCAATTACAACTTCTTTTGTAACAACAAAACTATCGTTGATTTTTTTGTCAAGAATTTCGACAACAATCTTTCTTGCTATACTATCTAAAATACGTTTTAATTCACGAACTCCACTTTCCCTTGTGTAGTAACGAACTAAAAATTCTAAAACTTCGTCAGAAATTTGAAATTGTTCAGGATTTAAGGCATTTTGTTCATACACTTTTGGAAGTAAATGTGATTTAGCAATTTCTACTTTTTCTTTAGTTGTATATGTTGATAGTTCAAGTAATTCAACACGATCAATTAGTGCTGCGGGTATTGAATCATAGTAGTTAGCAGTAGCGATGAATAATACTTTTGATAAGTCGTATTCTAAATCCAAGTAGTGATCTTGAAAATTAACATTTTGTTCAGGGTCTAATACTTCAAGCATTGCGCTTGTTGGATCGCCTCGAAAATCTGATGACATCTTGTCAATTTCATCTAACAAAATGACTGGGTTTGAAACTCCGGCTTTTTTAATTGCTGATATTATTTTTCCAGGCATTGCCCCAACATAAGTTCTTCTGTGTCCTCTTATTTCGCTTTCATCCTTAACACCGCCCAATGAAACCTTTATAAATCTACGATTTAATGATTCGGCGATTGATTTAGCAAGTGTAGTTTTACCAGTTCCTGGGGGACCAATTAATGTTAAAATAGGAATATTATTTACTGGTTTATTTGTGGTTTGACCTGTTTTATGAACGAATAATTTAGTATCAATAAATTCTTGATCGTTTCCTTTTATTGGTAAATATTCAGTTTTTTCTTCCTTGATTTTAAGATGAGTTAAAACCGAAATAAATTCAAGAATTCTTTCCTTTGGCTTTTCAAGTCCATAGTGATTTTTATTTAATACTTCTCTAACTTTGTTAATATCAATTAATTCCTTACTTACTTTTCTTCAAGGCAATGCAAGGATCAAATCGATGTATGTTTTAGATATATTAGCTTCTGGGCTAGATGGCATCATTGAAGCCATTCTTGATTGTTCAGATCTTAATGCTTCTAAAACATATTCAGGATATTGTTCTTTTAAAGTACTGTCTTTTTCAATGTCGTCAGCAGATTTTCCACCTTCATCATCCTTCAACATTTTTCTAATTTGACGCATTTTTTCTCTTAGGAAGAAATCTCTTTGTTGGTCTTCCATATCTTCACGTAAATTACTATTAATTTCGTTTTCTATTTTTTCAAATTGAACTTGTTTATTTGCCTTTTTAATTAGTTCATTGTATCTAGCTAAAACGTCATTGCTTTCAATTAAAGAAAGAAGTTCATCACTCAAAAAGTACTTTTTCAAAAACTTTATAAGTTGTCATGATTCAGTTTCATATTCAACTAATGATTGTTTAGGTTTGTTAGTTTCAAATTCTTTTTCTAAATCTCGAAATAAAACTGTTCCTATACCTTCGTTAATAACTTGTTTAGATAGTGAGAAGAACTTTTGTCTTATTTCTTCAATTTTTGGTAAATATGAATCATATGAATTGACTTGAATAGGCGAAATATTAACATAAAAATTATCCTCACTATTTGATGATTTTTCGTCAACTATTGAGATTTTATTAATATTAACCGGTTCTTTTGCTTCTAAATTAACATGATAAATTCCTGATTCTTCACTAATGTTAGTAATATTAACAAGTACGCCTACAGGAGCAATATCATTAATTGTATGTTCCTCATTTGATTGTTTATAATTTGGATTTAAAAATGTAATAACTATATTTTTATTTCCATTAAAAGCCTTTAATAAAGCTGACACTGATTTAGCTTTTCCAACTCTAATTTTGTCAGAAGTATATGGAATAATAATCTGATTTTTTATTGCTATATAAGGTAATTTCATTGTTATCTCCTTAGAAAAATGAATTAATTATTTAATATTTTATATTAAATGTTGTTTTTTATTAGTTTATTTACTAATTATTACTGAGGCGGGTTTTAAAATTCTGTTAAATAATGAATAACCATTGCTCTTTACTTCAAGAATTGTGCCAGAGGGTTCTTTTGATTCGATGCTTTCAGTAATTTGTTGACATTCAGGGTCAAATAAATCTCCTACTTTTGGTTCAATTATTTTTATTCCGTTATCTAACATTAAATTTTCAAAATCATTTTTAAGCATATCAAAGCCTTTTGCAAAATTATTAACAGCGATATTATCAGATTTTTTTGCAAAATTTAAAGCACTGTCATATTTTGTATATATCTTAATGAACTCTTCTAAAAAGTCTTGTAGGGCATATTTTTTTAATTCTGTTTTTTGTTGCAATAAATGCTCATCATTTTTTTGACGATGTTCATTTATTTTTGATTGTGCTTTTGCTTCCAATTCAATTACTTGATTTTTAAATAAAATTTCTTGTTGTGTCTTTTCTTGTTTTATTTTTTCATTAAGTTCGCCTAATTCCAAAAAATTTAGTTGCAATTTCTTAATATTTTCATTTAATTCTTGATTTTTAGCAAAAACTTCTAAATACTTAGGACTTGTCTTTTGAAAATCAATAATTATTACTTTAAAAATTTCATCATCAATTTTTAAAACTAGCTCACCTTTTTCGTTTTGCTTTCCTTCCAAGATAGTTTTTTCAATATTTTTATCTAAATCAAAGCCAACAAATCCCTTATATTCAATTTTTTCGTTTTTGTTAATTTTTCTAATACACTCTATATAATCATATTTTGAAATATTTTGACTCATTTATTCTCCTTTTTCTTCTTCTTTTTTAATAATACCTTCAAATAAATTTATCGCTTCTAATGATTCTTCAACATCTAAATTTTTAGGACCTAATATACTTATTTCTTTAATATTTTTGTGATTAATAAATTGAATTCTTTTTGAAATAATGTTTAAATTAGGATGTGAAAAATCTAGTTTTATATTTTTTTCTTCATCAAGATTGTTTTCAATTGCTTCTCAAACTGAGTGGTTTTCAATTAATTCTAATGTCTTAGCGATTTCTTCTCTGCTTATTTGTTCTGATAATATTATTACATTTTTATTAAACGATTTATTCTCCAATTTTTCTTCAAAACAAAAAATATTTTTGATAAATTTCTGCATTATTAATTCAAAATGTTTAACTTGTGTAGATAATATTGGGGCTAATGATTCAGTTTTTTTAGCAAGATTAATTAACGGAGTATCAATCAATCTTTCTTTAAATAATCTAATTGCAATCCTTAAATCATTTAATTCAATCCCACTTTCAAACTTAAACATTTTGTTTTGAACATTTCCTGAACTTGTAACAATAACAACAACTGCAGATTCTTCATCAAGCGGTGTCAACTGAATACTTTTCAATAATTCAGATTCATTGTTTGAAGTTGCAACTACGGCAAAGCCGGCCATTTGGCTAACGACATTTGCTGCTTCTTCTAATGTTGTGTTGATGCTTAACCTACGCTTAGATAAAAGATCTTCGAGTTTTTCGTTGAAATATTTTTTGGCATTGTAAAGCAAATTTTTTGTATAGTATTCAAGACCTTTTGAAGAAGGAATTCTTCCTCCTGAAACATGTTTTTTTTCTAAAAGACCTAGTTTTTCTAATTCTGCCATTGCGTTTCTTATAGTTGCAGAGCTACAAGATAATTTAAATTTTTCAACTAATCTTTTTGATCCTACTGGTTCGCCTGTTTTAATGTACATTTCAACAATGTATCTAAAATATTTAATTAGTTTTTTATCATAATTATTGCATATGATTAAAGACTTATTTGTGTTTGTGTCAGAAGAATTTTGCTCCATGATTGCCTCTTTCAAAAAAATTTTAGCACAATTTAGCACTTTAATGCGTTAATTGCTAATTTTTTAAAGATTAATAGTTTAAAAAAACTAGGAATTAACCTAGTTTTTAATTCAGTTTAACTGATATGGCGCGCCCGGCAGGAGTCGAACCCGCAACCTTTTGGGCCGTAACCAAACACTCTGTCCAATTGAGCTACGGGCGCATTGGAGCCACCAACCGGATTCGAACCGGTAATCAAGGTGTTGCAGACCTATGCCTTGGCCGTTTGGCTATGGTGGCAACACTGGCATGCATGTTTGCATATAGAATTATAATACATTTTATAAATTTTTAAAACAAAATTAGTAAAGAAAAAAGCGCAAGAGGGAACGCTTTAATACTATATATTTTCTTTAAATATGCAATAATTATATTATTTTTTTTAAAAAAATCGTTTTTTATTATTTTTAAATGCTTTATTTTAAACAAAAATTAATAATTTTAGAATGAAATGAACTTTTTCATTAATCTTAAGACTGTCAATTTAAGCAATGATTCAATCCGAGTTATGCCGTCATTGCCAGGCATTTTATTAAATATTATATGTTGCTTTGAAGAAGTAAAATCTATAGGCGAGCTCATAAAAGTGATTTTATTTTTTTGTTCACGTTCGCTCAATATATTAAATATGTTTGTAATATACCATTCCGGTTTGACTCCGATTGCAAAGCGATCTATGAATAAATAATCAACAGAAATTAAGTTTTGAACAATTGAATTAATTTCTGATGAATTTTTTAAATTATTAGACATGAATTCAAACAAATTATTGTTGTCAACAAATGCTACGCTTTTGTTTTTCTTAGTGCCAAATAAGAAGGCTAAAAATTTTAGAATATTTCTTGCATTAACAAGATTTTGATCAATAAGCAATAATCCCTGATTTGATTTCATTGAAGTAATATCTTTGATTTCATTTTTTAATTCAGGTGCAAAATTTTCTAATGATTGGTTTGTATTCAATACTATATATTGAGGTTTCTTTGCTTTTGCATTATTAAAATAATAAATTCATTCATTATCCATGCTTGTTATATTTGTTAGTCAAAAGTTATTCATTTCTTTTTGCTTTCTAAAAAAACCATCGTTGCTCAAATCAATTTGCAAAATTCCGTTATCATCAACAAAGAGAGTCCATGTTGGAAGTTGTTTATGCATTTCAATATAACTATAATATTTTTGCAAGTAATTCATTCCTTTTTGAATTTGTTTAGAATTCAAATTCAATTTTTCAATAATTGCTTTTACATTTGGATCGGCTAGAATTTTTGCTTTTTCAGCCTCTAAATCAATATTTTTCAAAGGAGAATTTTTAAACAATTCATCAAATATTTTGTTTTCCATTTTTTTGCTTTCTTATTTTTTAGTTAGTTCAATCATGTTCGTTGGCAAGATCTAAAAAACAATTTAAATCTTCTCTAGTAACTGATTCATCTTCATTTTTTTTGATATTTTTTAACTCTTTATTTGCATTTTGCTTTATTAGATAAACATTTTCTAATTCTTGATCAATTCTTTCGGCATCGTATAGATTTCTATGGCTAAAATCTTCCGCTATTTTTTCAATATATTTGCATACAACTGAATGGTTAATTTTAATTGAATAATAAATGAAGGCATTTATTGAAAAATTATTGAATTTCATTCTTTTCAATTTTCTAATCATTTCATTTTGGCTAAAACTTAAATTTGTTTTTGTATATTGACTAATAAATTGTTCGGGTTCTATCTTATCCTTTAATTCATCTAGGGTAATTGAATTAAAATTTAATTGTTCATTTTTTATATTTAAATCAATTACGTTTTCTAGATTAAATACTTCATAAAATTTTTTAGAAACATCATTAATTGTTGTTTTATCAAATTGATAATTTGCATTTAATTTTAATAATTCTTCATATCTTGATTTAGTAATTAATTTAATTAACATATTAGAAATAATTTTATTGTTGCTTATTTCATTTGCATTCAATGGAGCATTTAAACTAAAAATATAAACACCATCCTTGTTTAAATAACTTTTTAGTAAGCTTAGCGCTTCTAGTTTACTACGACATTCCTCAAATTCTTGTTCATTTAATAATAAAATATCTTTAATTTCAGAAAAATTAAAAAGTTTATTTTTTGTATAAATATTTTTTAAATTATATAAAAATTGATATAGAAATATGCTACTATGGCCTAAAATCGGAGCATAAAAAATTACTAGATTTTTAAAATCATTTTGTGTAATTTCCCTTTCGCTTTCAATAAAAAAATTTTCAACCATATTTAGCCCCTTTCTAATTAGTCGTGTACAAATATGATAGATTGCTTAGGAAATAAAAATTAATTTTTCTTAGGTTTTTTAACAATTTAACTACTTATTAAAAAGTTATTAACAATGCACCGTATGCCTTTTAGCCTTAAAACTAGATACTTTTTAAACTATTTTTATTTTCTAACAGCAAAAAGTGATTTTAAATGAATTTTCAGATTAAAAAAATGTAATAAAAAATAGAAAAATTTTTTTTCTTGAAAATAGCTTTCGCTATGTTTTCTTATAGTTTCGAAAAATGTTTTATTTAATAATATAATTGAAATATGAAATTAATTAACCTTCACTTAAACACTATATTTTCTTTTTTAGAAAGCACGATAACCATTAATGATTTTATTCAAGAATTGTTAAAGAATAATATAAAATATTTTGGCATTACAGAACATTGTAATTTTTATAGCATGCCATATTTTTTAAATGAGGCTAGAAAATATGATTTAAAACCTATTTTTGGTCTTGACGTAAATGTAAAAATAGAAAATAATTTGTATAGATTTATAGTTTACTGCCAAAATAAAGAATCTTTTATGAACCTAAAAATGCTTTCTTTAAACTACGGAAAAAAAGGCTATATAGATTTAGCTGAAATTAGAGATTATTCTAATTTAATATGAATAGAACATCCAATTTTTGGCTATTATAAAAAAACTAATAATATTTTAGAAATTCAAAACCATTATTTTGCAATAAGCCAATATGATATAGACAATAGTGAAATACAAAATATTTTTGATAAGTGTTTATTAATTAATCACAATTCAATATTAAAATATGATGATAATTCAATAATTAAAACAATGTCAAATATTTCAAATAATAAAATATTTGAAAGTATTTACGAAGAATTTAAAGTTGATATTGAAACAAAAAATAATTCAAAATTAGAAGAATTAATTGAAAGAACAAATAATTTTGCTAAAAAACTTTATTTTGAAATTGAAACTAATGGTTTTAAAATGCCCAAATTTAAAAACGACAAAAATATTGCTTCTAATGAATATTTAAAATTGTTATTATTTAAATCAATAAGAGAAAAATTTGATAAGTTTTCATGAACAGATGAATACCAACAAAGGTTTTTAAAAGAACTAAAAGTTATTGAAGATCTTAATTTTGCTGATTACTTTTTAGTTGTTCAAGATTGAGTTAATTGAGCAAAAAATAACAATATTAAAATAGGGCCAGGAAGAGGTTCGGCAGCCGGTTCATTAATAGGCTATCTATTAAATATAACCGAAGTAGACCCTATTAAATATGATTTGATATTTGAAAGGTTCCTAAATTCTAATAGAGTTACTATGCCTGACATTGATATTGATGTTCAAGATAATAAAAGAAATGATGTTATTGAATATTTATTAAAAAAATATGGGCATGAATTCGTGGCAAATATTGTTACTTTTTCTTCACTAGGTAAAAAATCAGCGATTAGAGATATTTTAAGAATAAACAATATTGCCCCAACATTAATTAATGAAATATCAAAAAATATTGAAAATGATGAACAAGACCTGTTAGTTGAATACAATGAAAATAGAAATTTACAATTATCATTAGCAAAATTAAATCCTAATGATGAATCTTTTAGCACAAGAATATTAAACCAAGCAGCAAGGATATCAGGATTTTATAGACAAATTGGCACACATGCTGCCGGATTAGTAATTAGCGACCAACCCGTCATCTCAATAGCACCAGTTCAATATAATTCTGAAAATTTTCTTCAAACTCAAATAAGCATGGAATATTTAGAAGATTTCGGGTTAGTTAAAATGGACATACTTGGTTTGAAGACATTGACGACTATTTCAAATATAATTGATCTAATTTATCAAAATCAAAACCTTAAAATTGACTTAAAAGATATAGACTTAACCGATCAAAAAACATTAGAGTTATTGAGCTCTGGCCACACAAGAGGCGTTTTCCAAGTTGAAAGTCCAATCATGATAAAAGCCTTAAGACAGGTAAAGGTAAATAATTTTGAAGATATTGTTGCAATAATTTCATTAAATAGACCGGGTCCAATGAAAAACGTTTCGGTTTATGCAAAAAGAAAATTCGGACTAGAGCCGATACCAAAAATAAATAATGAATATGATAAAATCCTTGATTCTACTTATGGAATCATTGTATATCAAGAACAAATTATGAAAATTGCTCAAGTTGTTGCTAAAATGACATTCATTGAAGCCGACAATCTAAGAAGGATTATTTCTAAGAAAAAAGTAAATGAAATGGAAGATATTAAATCTATTTTTATTGAAAGGTCAATGAAAAATGGCTATGAAAAGTCTTTGGCTACAAATATCTTTAACAGCATAGAAAAATTTGCATCATATGGATTTAATAGATCTCATGCGGTTTCATATTCAATTATAACTTTTCAAATGGCCTTTTTAAAAGCTCACTACCCCTTAGAATTTTATACATCATGTATAAGCTCGGCACACGGCTCCCAAGATACAATTGCAAAGTTCGTTAACGAGGCAAGTGAGTGTGGGGTAGAAATAGTTTCTCCTGACATTAACCTTTCTGAATCAGAAGCTATAATTAAAAATAAAAAAATTATATTGCCACTTACACTAATTAAAGGTCTTGGGCCTGAAATTGCTAATTCAATTGTTGAAAATAGATCAAAATATGGCCCATATAAAAATTTCCTAGATTTCTTATTAAAAATTTCTAGCTCTCAATCAATTGGCAAAAGTACAATTGAATTATTAATAAAGGCAAACACTCTTAGATCATTTAATTATAATCAAAACACATTGTTGAATGAGATTCAAAAAAACAATACTGATACAATGTTATTTATAAAAATGTTTAAGAATAACATTGAAGGTGTTAGCTCTAATATAATAAACTCTTATAAACCAAGTGAAATTCTTAATCAAGATATCATGTTTGAAAAGAAAAACGAAATTGAATTATTAGGTCAAGTTTATAATTTTAATTCAAGCGCAAGCAATGGAATGCCTGGACAAACTAGTTTTATAGACATGCACGTTGGAAATGAATATTTAGCAATCGTTAACTGTACAAACGTAAAGCAAAAAATTTCTAAAAAAGGTAATAACTATTTTGAATTAGATTTAGAAGCAAACATGCAAAAAATAAGAGCATTTTATTTTACATCAAATCAAGAAATACTAAATGTTAAGGGAAAAACTATCAAAGTAACTCTAGAAAAAAGAAATGAAAATATTTTTTATTTGCGTTCAATAAAGGAAGTTTTAAATGAAGAAAGATAATGAACAACTGTTGATTATAGATGGTACATTTTTAGCATACAGATCTTATTATGCAACATCTTATAACGCAACTCAAATTTTAACCACATCTGAAGGTTTTGAAACTAATGCGATAGTAGCATTCTTTAAAACACTTTTTTTATTACTAAAAAAATTAAATCCTAAATATATTTATATAGCCTTTGATTCGCATGTTAAGACGTTTAGGCATGAATTATTTCCTTCTTATAAAGATGGTAGAAAAAAAGCACCAGAAAGTTTTTATCTACAACTTGATTTAATTCAGGAAATATTAACAAATATTAATATTCAAAACCAATATTATAATGGCTTTGAGGCTGATGATATAATTGCAAAAGTTAAAAATAAATTTAATTTGAATACAATCATTTTTTCAGGAGATCAAGATTTAAATCAACTGATAGATGAGCAAACGGCAATCATTAAAAAAGATAAAACTGGTTCTTTCTTTTTTCTTAATTCAAAGAATTTTTCAGAATATTATGATTTTTTGCCAAATCAAGTTATTGATTATAAGGCATTAGTCGGTGATAACAGTGATAATTTTAAGGGTGTTGTAGGAATTGGACCTAAATCTGCTAAAACTTTATTAGAAGATTATAAGACTATTGAAAATATATACAATAATATTGAAAAAATAAAACCTTCAATTGCCAAAAAATTAATGGAATATAAAGAAAATACGTTTCTCGATAAATATTTAGCAACCTTAAGGATAGACTTTGATTTGCAACTTCCAAAATTAGAAAATTTAGCAATATCTAATATTGCAATTTCAAACAGTGCAAAACAAATTATTGACAAATTTGAACTATATTCTCTGGAAAATTCTTTGAAGAATTTAATCAATAAAAAATAACAAAAGTAGGGTTTTTCCTACTTTTGTTATTTTTGAAGTTCTTGCAACGTTTTTGCTACTCCAGATTTTTTATATGAATATCCTACATAATCTGCTTTTTGCTTTATGTTCTTTGTGGAATTTTTCATTGCTACAAAGCCACCTAAATATTTTTGAGTAACAACATCATTACCCGAATCGCCTAAGTGAACTGCTTCTTTATAATCAATATTTAACAAATCGCATACAAATCGATCGCCAATTCCTTTATTGGCATTAATATCGCTAATTTCAATTGAATATCCTTTTGATACAATATGTGTTGAAATATCATGAAAAGCATTTAATAATTCATCTCTAAGTTTTATTATTCCCTTTTTTGACTTCCCAAAAGTTAAAATTTTTGTTGTTGCTGGCAATGATTGAATTTCGTCATATGTAACTTTGTTTAGTTTCTTTAATCAAGCTCTAGCAATTCTAAATTTTGTTTTTGTTCCATATATAGTATCGCCACTATTAAAAATAAAAAACATTTTTTGTGCAATTAAAATTTCAATTATTGATTTCAATGTATCAGGATTAATTTCATGGCGTTTCAAAACATTATTATTTTTATCTACGATTACTCCACCATTTTGGCAAACAGCAAACGGTGCATTTATTTTATTAGTTAAATCCAAAACAAACTTTGAATTTCCTCTACCTGTTGACAAAATAACAGGGATTCCTCTATTGTTGAAGTCCTTAGCAACCTCAACATTTTTTTCGCTTATCATAATTTGTTTTTTGCGAGGCAAATCTAGATATGTGCCATCCATATCCAAAAAATATGCCTTAGGTTTAAAATTAATCATAATTTTCCTACTTATTTAAATTGTTTAAAATTTCTTCAACTGGCAAGTATCCTACACTTTGAGAAACTAACTTACCGTCTCTATAATAAAATAACGATGGAATATGAGATACATTCATTTCTCTTGCTAAATCAATGAATTTATCTACGTCCAAATCAAAAACATTAACATCTGAATTTTCTTCTGCTAATTTTTCTAATTCTGGGCCAATCATTTGGCATGGACCACATCAAACTGCTCTAAATGCAACAATTGATTTTCCTTCTAAATATTCATTCTTTTTGAATTCTTCAACTGATTCAATAGTTTTATACATAATATTTTCTCCTTTAATATGCTCTTGCAAATAATACAAATCTTTTTGTTTTTCTATTAGTTCAAAAACAAGTATCATTTGAAACTTTTATTGGTTCTTTAAATGGAATACACCTTGTTGATGCTCCTGTTTCTTCTTTAATTTTTACTTCATCATCACCATTGCCATCAAATGCAGCATACACTCAGTGACCCGCAGCAATTGCTTCCTTAAATTCTTGATAACTATTGACAGTTACAATATTATTTTGTAAGCGTTGTTTAGCACTTTCAAATAAATTGTCTTGTATTTTTTGCAATAAGAGACTAGCAATATTTGCTGCTTCAGCAATTGTTGAAGTAATTTTTTCTAGATTATCACGTCTAACGAATAATACACTATTATTTTCAATATCCTTTGGACCGATTTCTATTCTCAATGGTGTACCATGAATTTCTGAATTTGCTGCTTTAAAACCTGGACCTTTGTTTGAAGAATCTATGTTTGCAACAATATTTTTTGCTAATAAAGCATCTTTTAATTCAAGGGCAAGTTTGTGAACTCGTTCATCCTTTGAATTAAAAATTTCTAATATATCAACTTGAGTTCTTGCTATTTTAGGTGGAATGATTATTCCTCGATCATCACCATGAGTCATAATTAGCGCGCCAATTAATCTTGTTGTTGTCCCTCAACTGGTACCAAAAGGATTAATTAATTTATTTTCATTGTTTTTAAATGTTATATCAAATGCCTTAGTAAAATTTTGTCCTAAATAGTGGCTAGTACCTGATTGAAGTGCCTTTCCGTCCTTCATCATTGATTCTAAGGTGTATGTTATATCAGCGCCAGCAAATTTTTCATGATCCGTTTTGCAGCCTTCTACTACTGGAATTGCTAAATTTTCTTCAATAACATCTTTATAAACCTTGTGCATAGTCTTGGCAAATTCAATGGCCTCTTCACTACTTGAATGGATAGTATGACCTTCTTGTCACAAAAATTCCCTTGTTCTTAAAAATGGATTTGTTGTTTTTTCTCATCTTAAAACATTAACTCATTGATTATAAATTATTGGCAAATCATTGTAAGATGAAACTTCATTTTTGAATAAATCAGCAAACAACGTTTCTGAAGTTGGTCTTATAAAAAACTTTTCTGCCAATTTTTTGCCGCCTACTTCAGTAACAGTTGCAAGTTCAGGGTTAAATCCTTCAACATGATTTTTTTCTTTTTGCAATAAGCTTTCTGGTATTAAAAGAGGTAAATAAACGTTTTGAACGCCTCTCTTTTTAAATTCAATATCCATTTTTGCTTGAATATTTTCTCAAATAGAATATGCCAATGGTTTAAAAATAATGCATCCTTTTGTTGCACCATATGCCATTAAATCCCCATTTTGGATAACGTCAGTATATCAACGCGCAAAATCTTCATTTTGTGGTGTTATTTTATCTAATTTTTTCATGTAATTAATTATAAATAAAAAAGATAAAATTAAAAGCACAATGTGCTTTAATTTATTTTTTAGATTAATTTATCAAAAATATCTTCACCTGTTTGAACGGTTGGAACATTGAAATTTCTTGCAACAATATTTCCTGCAGTTCCCAAGCCTTTCAAAGGTTCTTTAAATACACATGGTGATTTGAATTTCTTTGAAAATATTGTCAATGGCAATGCTTCTCTTGTATGATCAGGGCCTTTTGTTGGGTCATTTCCATGGTCAGAAGTTATAATTAACAAGTCATCTGATTTCATTGCATTTATCAATTTTGTTAATTTAATATCTAACCTTGAAATATTTTGTGAATATCCTATTGGGTCTTCTCTATGTCCGAAATGGCTGTCAAATTGAACAAGATTTACAAATACAAATTGGTTTTTGGTATTTGATGAAGCAATGTCAATTGCAACATCCATATTTTCATCATCAGAAGCTGGTCCATAAACTTTGTCAATACCAACATGGCTGAAAATATCATTAATTTTACCAACAGCAACTACTTCAACGCCTGCTTTTTGTAAATCTTCTAGAATAATATGTCCTGTTGGTTTATTAGCAAAATCATGACGATTGAATGTACGGGTGAACTTTCCATTTTCATAAACAAATGGACGTGAAATAACTCTTGCTACATTTCATTCAGGTCTTGATGAACAAATTGCTCTTGCTCTCTTTGCATATTCAGTTAGTTTTTCAACACCCAATGTTTTTTCATCCCCAATAATTTGCAATGTTGAATCAGGAGAAGTGTAAATTATCATGTCTCCTTTTTCAATGTGTTGCAAACCTAATTCGGCCAATATAACTGTACCTGATGCATTTCTATTTCCAATAATTTTTCTATTATCAAATGCTTTTGATAATTCTGCAACTAAATCAGCAGGGAAACCTTTTTCAATGAATTGTGGGTTTGGAACTTTTGTATAAATTCCCATCATTTCTCAATGTCCTGTCAATGTATCTTTTCCATTTGACATTTCATAAATTTTTCCCATATATGCCAATGGTTTTTTATTTCTTGGTACATGTTTTGAAGTTTCTGCTATTTCAGTAATTCCTAATTTTGTTCATGTAGGAATTTCTAGTGGCAATGCTTCTGACGCATGCAACATGCTATTTGCACCTTCGTCATGAAATTCAGCTTGGCGTGGTTCATATCCAATGCCAAGACCATCTGTAACTATAAAAAATATTCTATTAAATTTTTGTTCTTGCATAATTAGTACTCCTTTTTAGCAATAACAACAATAATTTGAATCTCATACTTATTTTTTAAATTGATTTTAATTTTAAATAAAAATAATTAATGTTTTAATAAGTTAGATAAGTAATTTTACTATTAATTTACTCACTTATTATTTTATTACAATAATGAATTTAAATTTATTAACTTTCAATGCATTAATAGACTTTATATTGCCCGGCGGGCATTCTATTGATTTTTTTGGCCTCCTATGTGATAAAATTAATATGTTAGATTTATATATTTATTTAGCAAAAGAGGTAATATGAAATTTAATGAACTAAGAACTAAAATTGTTGCAACAATTGGCCCTTCTTCAGATAATTATGACACAATGAAGAGTTTAATTGAAGCCGGAGTTACTACAATTAGAGCCAATTTTTCACATGGCGGTCCTGAAACTCATGTTAATAAATTTGAAATAGCAAGACAAATTTCAAAAGAACTAAACGTCCCAATCTCATTAATGCTTGACACAAAAGGCCCTGAAATTAGAGTTGGAAAAATGGAAAATGATGGAGTTACAATCCCTGCAAATCACATTTTAAAAATTAAAACAGATGAAAATTCATACAAGAATTTTTTAGGCACCCAAGATCAAATTACTGTTTCATATAGAATGGACAACGATCTTAGAATAGGGGATAAAGTTTTAATTGATGATGGAAAATTAGTTACAACTGTAAAAGATATTAAAAAATTCGAAATTATTGTTGAAACAGTTAATGAACATTTCTTAAAAACTAATAAAAGAATTAACATTCCTGGTGTTAACTTCTCACTTCCTTTTTTAAGTCAAAAAGATATTGACGATATTATATGGGGTGCGAAATACAAAGTTGATTATATTGCAGCATCATTTGTAAATTCTGCTAAAAATGTAAAAGAAATTAGAGACATATTAAACGCAAATGGTGGAGAAAATATTCAAATCATTTCAAAAATTGAATCTAAGATGGCAATCAATAACATTGATGAAATAATTGAAGCTTCAGACGGAATTATGGTAGCAAGAGGTGACCTTGGACTTGAAATTCCTTACTATGATGTTCCTTACTACGAAAAGAAAATAATTCGTAAATGTAGATTAGTAGGCAAGGAAGTTATAGTTGCAACTCAAATGCTAGATTCAATGGAAAACAACCCTATTCCAACAAGAGCGGAAGTTACTGACGTTTATTATGCAGTTGAATTAGGTGCAGACTCAACAATGTTATCTGGAGAATCTGCAAGTGGTAAATACCCTGTTGAAGCAGTTAAAGTTATGTCTAAAATTGCAAAAAGAGCAGAAAAAGAATATTACAATGAATTATTCTATGGCAAACAAATAGAAAAAGTAGCAAATCATCCTACTTCAAGAATGCTAATTGCTTATGAAGTTGCCAAAATCCTTCAAAGCGGTGAATACAAATTTGCCATTATATTAAGTCACACCGGAAAACTATTAAAAGAAGTTGCTAAATATAGACCAAACGCATTTATTGTTGGAGTTGTTAGCGACAATAGATTGGTTTGAAGCTTTGGAATTACTGCTGGTGTATTCTGCGAAAAACATTCAGAAGCAGTAAGAGCATTAATCAAAAACAATCACAATGCTGCTTATAAAATTTTAGGCGAATATGGTGCTAAAAAAGGCGACAAATATATTGTAGTGGATAGTTTAAATATCACAACCCATGAATACTAATAAAAATTATTAATTTCAAAACGAAAAATTAGGCTTTAACTAGGCCTAATTTTTTTGTCTTGGCTTAACCGGACTTACTTTTTTATATCGCTTATTTCTTATTTTTAAAATAAAAACATGCTTTTTATTTGTTTTGACTACCTTCTAAAAGTCGGTCGTAATGAGATAATTGCTTATTTTTTTGTGACCATATTTATAAATTTAAATTAATTAATTCAGAGAATTTTTTTATATAAATATTTTTCCCAACATTATTTTTTTCAAGCATTGATTTATATGTAGATAAAAATACGGATTCTAATTCTTTTTTTTGTTCTTCGTTAAATTTTAAAAAATTTACAAAATATAAATTAATTTCCTTTATATTTTGATTTGCGTTAACTAGGTTTTGTGCATGTTTTGATATTGAATAATTTTCACCATCAACAACTTCCTTCTCTGTTTTTAGTATAGTCGTTTCAACTATATACTGAATGTTGTTTTTTGAATCATAATAATCGAAATCTGCCGAATGTCCGGGAGCAGAAAAAATTGGATACAAATTCTTTGTTAATTTTGTATGACATATACTTTTAAAATTTTGCGGATTAATATTAAGGGAATATGTGAAGAATAAATTAACAAAATATTCTGCTAACGCAAAATTTGGCACGTGTTTTAGGGCAATGTATTTTTCTGATGGTGCTTCTTTAAATTCAAATTTTTCACTTTGAATTTGATTTAAAATATCTATTACTTCTTCATATTTAAATGGAAACTTGATATTTTTTTCAATATTATCCACCTTAAAAATTGGCAATGAATAAAAATTGTCTTCATTTAAATATTTCAAATTTTCTAAATTAAAAAAATTATTCAATTCAGATTTTAAAATCGTTTTTGAATTGCTATTATATAAACCCAATTTACACATTCATCTATTGAACATGTCCATATATTCTTTTTCGATATTAAATTCAATAGAGATTTTTTTTAAATCTAGCAAAAAATCTTCTTGTAAATGATTAAATATGTAAAAATTTATTTTATCTAAATAGTTTACATTTCTTTCTTTTGGGGGAATATTTAATAATCTTTTTATATATTTAAATTCTTTGGGTTTCAATTCTAAATAATCACTATATGACAAAGGTTCTTTGATTCTTAACAAATTAAAAATTTTTTCTAAAATCTTATATTCATTAATTTTCTTTTTAAATAAAGACTTAATATTAGTTATATTAGAAACATCTGCAATCTCTTCTAAAATTTGCTGTTTATTTTTTGTATATAGATCCTCAAATTTATCTTCTTTATAATCAAAAACCGCAAATGCTCAATTTAATTTTTCAATGTTAATCGATGATTCATTATAAGAAATTATAAAATTTACCATTTCTCTGAATTCTTCTCAATATAAGTACATCTTTTTGAAAAATAATTCCTCAACCGGGCTGTCTGTGTCAAAAAATTTTTTTGGTTCAAAATTAAAAACTAAATTTTTAGAATTTTTTAGAAATCCTCATTTTTTTAAATGACTTATCACGAAATTAAATCTACTTTTTATTTTTTTATCATTGCTATAATTATAGATTTTTTGATTTTTAACATATTTTTTAAACTCTTCGTCTCAATTTACATTGAAATTTTTTTGAAGATTGTTTGACAAATTATTTTCAATAGATATTCTCTCATAATTTACTCAAGATAAAATAGAAAAATATTCATTTATAGGTTCAGCAACTCTAAATTCAGTACCACCAAATTTATTAAATCCCTGCTTTTTCATAATTAGTTACATAAACTTCGTCATCTAGTTGATCTCTATTTTTTCTTTGATAATTTGAATTTGTGTATTGCTTATTTAAATGAAAAACTTTATATTTTTTTGACCATTCAATTAATTCAGTATTTGATTTACCCTTCGATTGAAATAAATTTGATAATCCTCATTTTATGTTTTTTGAATCTAACAAATCTAATAATTTAAATAATTTTCTGTCTTCTTCTAATGTTCAGGCCTGATTATATATTGCACCCGAAATTAAGTATGGAGGATCAAAATACCAAAAATCGTCTTCTCTTGTTTCTTGCATTTTCAAATATATAAATTCTTGAAAATCCAATGAAGAAAAATCAATATTTTTTCTATTTAAAGTTGAAGAAAAATCGACAAGGCATTGTTTCCTTGTTTCATTTAAAATTTGTTTTCCGGAAGGAATATTAAATTCTCCTTTTGAATTAAATCTTATTTGACTATTAAATCCATAAATTATTAGAACTAATAAAACTATACAAGATTCATATGAATACTTATTTTTTGATAAATTATATTCACTTTTTATCGAATTATAAATTGATGAATTTACTCTTTTCGTTACGGGATCTAATTTATCAATTGAGCCATACTTCTTATATATATATATATATATATATATCATTTAAAATTTTTATAGGGTTAGTATTTGAGAGAAGTTTTACAATATTGCACACTTCTTTTTGCTTATCATTGTAAAAATATTTTTGAGCTATAACATTTGCAATAACCGTTCCTCCTCCGGCAAATGCATCATAAAAATTTCTAATATTATTTGGAAATAATTTTTTTAATTGTGGTAATAATTTATATTTATCGCCCAAATAATACAATGGTGATCTATTCATTTTATTCATTTCTAGCTCATACCTTTCTATTCTTTGCTTTGCAGCACAAAAATATTTTTTATCTATCTCACATCCAATAAAATTCCTTTGCAATTTTAAGGCCGCAACGCAAGTCGAACCAGAACCCATAAATGGATCAAAAATTCAATCATTCTTATTTGTATGAATATTTATAATTTTTTCCATTAACTCTACCGGTTTTTGAGTCGGATGCAATTTATCAATTGATTTAGAAACGATTGAATTTTTAAATTCCGGTCTTAAATATTTTTTGCTTTTTTGTTTATTAAATGTTCATTTTTTGTTTCCTTTTACTGCTCAAATAGCAAATTCAAAATCTGTAACATATCTTCTTTCTACATTTCTAGGCATAGGGTTATTTTTTACTCATCTTAATAAATCTTTTATAAAAAATCCTTGATCTTCTAGTTCCTTGCTTATTTCGCCAAAATTTTTGTAATCATTAAATATTATTATTGAACCGCCATTTTTTATAAATGGGCTAATTTTCTTAATTCATTTAGTTTGGTCAAATTCATAATCTCATTTCCCAAAGTCTATTCCTGATCTGCCAATACTTTGAAAGTTATTTTTTTTAGATATATTATATGGTGGATCAGTTATAACAGCATCAAATAGTTCTATATTTAAATTTTCTTCTTTTAATTTCTTTATATATTCTAAGCAATCTTTATTCTTAATAATTCAAGTCATATCATCATCATAACAAAAAAAACGAAAATAACGACTATACAAAATTAAATTTTTTATAATTTTAGTTATAAAAATTTTCTATATAAAACTTTAAGTTCATATAGGAACAAATAGATATTCAACTCTAAATTTTCTTAATTCTCTTTTTTTATACACTTACTTTAATATTATAAAAAACACCAAGTGTTAAAATAATATACTTGGTGTTTCGCTTTAAATATTCTATTATTTAATAACTTCTTTGCCCATGTATGGAACTAAAACTTTTGGAATTGTGATTGAACCATCTGGGTTTTGATAATTTTCTAAAATAGCAGCAACAACTCTATCAATAGCTAAGCCTGAACCATTCATTGTGTATGCATATTTTGTGTGACCATTTTCATCACGATATCTAATTTTTGCCCTACGACCTTGGAAATCTCTGCATATAGAAATTGAGCTTGTTTCTCTATATCTTTGTTCTGATGGAATTCATAGTTCTAGGTCAATAGTTTTTGCTGAGCTAAATCCAATATCGCCAGTTGAAAGCATCATTTCACGGTATGGGATTTCAAGCAATTCAAGAATGCTTTTGGCATCTTTTACCATCAATTCTCATTCTTTCATTGCATCTTTTTCACTGGTTATTTTTACAAGTTCTACTTTATGGAATTCGTGTTGTCTAATTAAACCTTTTGTATCTTTTCCACTACTTCCTGCTTCTGAACGGAAACATTTTGTATATGCAACAAATTTCTTTGGCTTAGACAAATCTAAAATTTCGTCATAATAATAATTTGTAACCGGAACTTCTGCAGTTGGTATTAATCAAAGGTCATTGCCTTGCATTTTAAATAAATCTTCTGCAAATTTAGGCAATTGTCCCGTTCCATAAAGCATATTTGAATTAACTAAATGGGCAGGCATTAATTCTTTATATCCATTTGCAATGTGCGTATCAAGCATAAAAGAGGCTAAGGATCTTACTAATTTAGCACCATCATTTTTGTATAAAACAAATCTTGTGCTTGCTAACTTCACTGATCTAACAAAATCAACAATATCTAATTTTGTTGCTATTTCATAATGTGGCAATACGCCTTTTACTAACCCTCTTCCTAAATTTGGATGTTCTTTTAAAACTACGTTGTCATCTTCATCTCTTCCCACAGGAACTTCATCATATGGCAAGTTAGGAATTTGGTATAGCAATGTATTGACTTTCTTTACCAATGTGTCAGCTTCTTCATCAATTTTTTCAATTTGTTCTTTAACTTCAGCAACTTGTTGTAATAATTCCGAGCAATCTTGCTTGTTTCTTTTATTAATTCCAATTTGTTCACTTAATTTATTTCTTTTGGCTTGCAAAGTTTCCAAATTATGAATTAATTCGTTTCTTTTTTGAGCGTCTTCAAAAATTTCTTCTATGATAGCAATATTGAAATTACGTGTTGATAGTTTTTTAATAACTTCATTTTTGTTACTAATAATATATTTTAAATCTAACATTTTTAATCCTCTTTTATTTTTTTAAATGCTATTATATTTTACAATATTATTAAATAAATGAAATACTTAATTTTTGTTAAACATATTTAACTTTTGCTTATTTTATATATAAAACAATAATTAATTATTATTGATAACCAAATTCTTATATTTGTTAAAATTTTAAATATGTTACAAAATAGCAATATTCAAGGTAAATTTAATAAAATAATGTGAACATCTACAGACGGTAGAGTTGTTATAGTTTCGTTTTTGGTATCTTCAAACGATGGTTTGAATCCTGTTAAAGTTAATAAATACAATAGTATTTCAGTAACTTTAAAAAACAATTTATTTTCTGAAAAAAACATTACCTTAAACAGCCACATTTATGAAATATCAGTCTCTAAAAATAGTTTTTCAAAATATCCAAATAGTTATATTGCAACAGACGTTATTGAAATAAACGAAGATGAAGAATATAAACTAAGCTATGTTATTAAATTTTTAAAAAGCAGTCATTTCCCTGGTATAGGTGAAGAAAAAGCAAAACTAATTGTAAAAGATTTAGGGATCGATGCTTTAAGAAAAATGGTTGAGGATAATTCAATTGATTATTCAAAATATTCAATATCAGAATTGACATGAAACTATGCTGTTGAATTTATTAAAAATAATCCAACATTAATTGAGGATCAAATGTTCTTTTTAACAAATAATTTAACGCCTTCTTTGTATGAAATAGTTGCTAAGAAATTTGAACATTTTAAAGATTTTATAGATGCTTACAAAGATAACTTTTACTTATTTTATTTAGACAATGAACGTATCTCACTTGAAGATATGGATAAACTGCATAATATCTTTAACAAATTTGAACATCCTTTTAAGCCTGCAACTCACTTGTACAAATCATTTTTAAAATATTTATACAATTCCGGAAACACTAGAATTTTAAAGGATTGTTTATATGATCAACTAATAAACTTTGCAAGTCAAGACACTGAATGACCAAGGGATATTGAAACATTCAATAATAGCGTTGAATTATTGGTTCAAAATAAATATTTAATTGAGATTGAATACGAAGATGGTACATACCTTAGCGCCAAAGATGTATTTGATATGGAAAAATATATTGTCAAAAGATTGAAACATATTGAATCTGCTTCAAACCCTAAATTCATTAATTTCGTGCCAAGTGAAATGTTCCACCCTTTGCAACTTCAGGCAATTGAAACAGCATTAACTAGCAAGTTAACATTGATCACAGGAAACCCCGGTACCGGAAAAACGTTAATAACCAATGAAATAATCAAACAACTACTTTCAGTTTATGATGAAGATGATTTGGCAATAGTTACACCAACCGGTAGAGCAACTATCAACATAAACAATATTCAAGAAAAAACAAATGCAGTTACTATACATAGTTTTTTAGAATGAGATGTTGATAATGACAAATTCATGATTAATGAAAAAAATGCAAAACCGATTGAATGTTTAATAATTGATGAATTTTCAATGGTAAGTGTTGATTTGTTTTATTCACTACTTAAAGGAATAAAAAAGAATTCGTTGAAAAGAATTATATTAGTTGGCGACAAAGATCAATTGCCTGCCATTGGCGCTGGTTATTTAATTAGGGATTTTATAGAAAGCAATATTTTTCAAACAATTGTTTTAAGTAAAATTTTTAGACAAGCAGAAAATTTTGACATTATTCAAGACGCTCTAGATATTAATAAAGGCAAACTACCCGAATTTAAAGGAAATAATTCTCAGTTTATCGAAACGAAACAGAGTGATCTAAAGTCAATGTTGCTCTTTAAATTAAATGACTTATTACAGCAAGGATATAGCAAGCGTGATATTGCAATTTTAAGTCCAATATATAATTACGAAACGGGAATTGACCAAATAAATGAAACATTAAATAATTTCTTTAGAAAATTAGATGGCAATGAACAAATAAAATATAGAGACCATATTTTGGCCATTGATGATAAGGTAATAAATTTAGTTAATGATCCAAAAACAAAAGTGTTTAATGGAGAAATCGGTTATATATCTCACTTTGGCTTTGAAAACAAAAAAAATTCTTTTGAAAAGGTTTTAGCAAGTATTTCTGTTGATTTTGAAAACGATTCAAAAACTGTTTCTTATTCTAGAAAAGATTTTTTTGCTAATACATACCCTGCATATTGCACAAGCGTTCATAAATATCAAGGTAGTGAATGTAAATGCGTTATTGTAGTATTATTTTCAGAGGCTAAAAAATTATTATCAAAAAAACTTATTTATACCGCTATTACCAGAGCAAAAAAATATTGTGTTGTTATTGGAGAAAAAGAAGCTTTGATAACCGGTATAAATAATGATGATGATTCAAATAGAATAACCAATATTAAATATTTATGGAGGAACGAATAATGAAATTAATTGTTGGACTTGGAAATCCTGGTGTTGAATATGAAAAAACACGCCATAACACAGGGTTTATGGTAATTGACAAACTAGCAGAAAAATTGCATACTCCCTTAACTGAAAAAAAGTTTAATGGAATTTATTACATTGACAAAGAAGTTATTTTAGCCAAACCGTTAACCTTTATGAATAATAGCGGTGATTTTGTTAGAGCAATAAAGGATTATTACAATATTGTAGATGATGACATTATCATTGTATATGATGATTTAGATTTACCAGTTGGAAAAGCTGTTATTAGACAAAAAGGTTCGTCTGGTGGACATAATGGAATGAAAGATATAATTGCTAAATTAAAAACTGATGAAATAAAAAGATTAAAAATAGGAATTGGACGTGGTCAGAATGTAATTAATTATGTTCTTGGCAAATTTAGCTATCAAGATTTTTGCACAATTGAAAAAATAATTGATATTGCTTCTGATGCATTAATTTCTTTTATTAGTAATGATATTAGATTTGTTATGAATAAATTTAGTGGTGAACTATATCGTGAAAACAAATAATTTAGTTGAATTACAACGTGACTTATTAAATGAATTTAATAAATACAATATTAATACCAATAAAAAATTTTTAATCGGAGTAAGTGGTGGCCCTGATTCAATGTGGCTACTTAATTTGCTTAAATCATTGAATATTGTTGTTGCACATGTAAACTACAACAAAAGATATGATAGCAATTATGATGAATCATTAGTTTCACATTTTTGTTTAGATAACAATATCCCTTTAGAAATACTCTCATTACATAACAAATATTCTAGTGGTAACTTCCAAGCTATTGCTAGAAAAGAAAGATATGATTTTTACTTTGAAATTTCTAAAAAATATCAATGTGACTATATCCTGCTTGCTCACCAAAAAGATGATTTTTTAGAATCATCTATTATGCAAGAAAATTCAAACAGAAAACCTTTATTTTGAGGTATTAAGCAACTTAGCATACTAAATAATAACGTCATTTTTAGACCAATGTTAAATTTATGAACTAAACAAGAAATTATAGACTTTTGCAATCAATATTCAATATTATTTGCAACTGATTATACCAATGCCGAAACTCATTACACTAGAAATAAAATTAGATCGAACTTAAAGAAATTAGATCCAGAGCAAAAACAAAATAAATATCAATTTTTTGTCAATAAAAATATTGAAAATGAACATATTGAAAAACAAGTTTTAGAAAGTTTTGAAGATTGAAAAAATAATCAATTCAATGTTGATAAATTAGATAAGAATTTTGCTTATATTGACCAGTTATTGTTTTATACATTAAATCATTTTTATAACAATTTAAAACTTTCATTAAACAAAATACACGGAATTAAAGATTTTTTATTTTCGCAAAATAGAACAAAGAGTTTTAAATTAAACGACGAACAATATATTCATAAAATTAAAAATTTACTAATATTTAAGTAATTTTATTATAATTTACATATTGAATTATAAACAAATAGAGAGGACAAATGAACAATCCAAAAGAAAGAAAACCTAGGAAAATTGGTTTGTTTGGAATAATTGGAATTTTATTTGCAATTATTGCAGTGATAACATTGGGATATATTATCAGTGATGTTGTTAAAGAAAGAATGTCTAACTTTCAAACCTTAAATTTTCTAGAAAGAGTTATTAAAGGAACTGCTAACCCAAATGATGGAAACTATATTTCTTCATGAGTTGAAGACCCTTATAACGATACAATAAATATCACAGTTAGAGGGACAAATAGTTTTTTAAGATCCTTAATTGGAAATAATTATTTTGTTGGCGATTCTTATAGTTTTATAGTAAAAAATGGAGGCTATATAGGTCGTTCAATATTTCAAGAATTGCCTGTATGAGCAGATTTAAGTGGAAAATCATATATAGAAGCATTGAATTCATGTGCAACATTTAATGGCCAAGTTTTAGGAAATATTGTTGCAAGTTCAATGCCAAAACCAAGCATTGCAAGCAAATATATAATTCCTGCTTTCTCTGCTTTTGTCGGCTTAGCATTTATTATTATCATTATGGCAATGTATTTTGGTAAGGGAAGAGGAATGTTAACTGGCGGTATTGATACAAATAAATCATTAGCGCAAAGAATATATTCAAATAAAAAATTCTCTGACATTGCCGGAAATGAAGAAGTAAAAGAAGAAGTAAAAGAATTAGTTGATTATCTTAAAAATCCTAAAAAATATGAAGCAGCCGGTGCTAGAATTCCAAAAGGAATATTATTAGGAGGCCCTCCAGGAACTGGTAAAACTTTAATTGCCAAAGCAACAGCGGGAGAAGCAAATGTTCCATTCTTCTTCATCTCGGCATCAAATTTTGTTGAAATGTTTGTTGGATTAGGTGCAAAAAGAGTTCGTGATATGTTTGCTGAAGCTCGCTCTCATGCTCCTGCAATAATTTTCATTGACGAATTAGATGCTGTTGGTAGATCTCGTGGAGCTGGAATTGGTGGAGGTAATGATGAAAGAGAACAAACTCTTAACCAATTACTAGTAGAAATGGATGGTATCAAAGAAAATAGTGGTATTTTAATAATGGCAGCTACAAATAGAACCGACGTTCTAGACCCTGCTTTATTAAGACCAGGACGTTTTGATAGAACTATTACCGTTGGCCTTCCTGATATAAAAGAAAGAGAAGCAATTTTAAAATTACATGCTAATGGAAAAAGAATTGCAAGCAATATTAATTTTGCTATGGTAGCAAGAAGAACACCTGGTTTCTCTGGAGCACAGCTTGAAAATGTAATTAACGAAGCCTCTTTATTAAGCGTTAGAGAAAATACCGGAGTTATAACTATTGAACAAATAGATGAAGCTATTGACAGAGTTATTGCCGGCCCTGCTAAAAAATCAAGAACAATTTCTGAAAAAGAAAACATTGCAGTTGCCTACCATGAAGCGGGGCATGCAGTTGTTGGAATAAAAATTAAAGGCGGAAATAAAGTTCAAAAAATAACTATTATTCCAAGAGGAAAAGCTGGCGGATATAATTTAATGATGCCAGATGAAGAAAAATACAATCGCTCGAAATCAGAATTACTAGCTATAATAACTTCGTTCATGGGAGGACGTGTAGCTGAAGCAATTATTTATGGAGCAGATAATGTTTCAACTGGAGCAAGTGACGATATAGCTAAAGCAACAAATATTGCTAGAAAAATGGTTACTGAATGAGGTTTATCAGATCTAGGACCAATTAAATATGAAGAAGATTCTTCAAATCCTTTCTTAGGAAGAGACTACATGAAAAATTCTTCATTTTCAAGTAAAGTAGGCCAAGAAATTGATGAACAAATAAGAAAAATTATTTTAACCGCTGAAAAGAAAGCACATGAAATAATTACAGAAAATCGTCAATTGTTAGAATTAATTAAAGACGCATTAATTATTAAAGAAACTATTGTTGCTGAAGAAATTGAATATATAGAAAAACATATGGAATTGCCTCCTGAAATAACAAATACAAAAAAAGAACTTAAAAATGAATATTCAGAAGAAGATTTCAATGAATTATTTAATGAAGTTGCTGGACAAAAACAAATTCAAGAAGATAAATACAAAGCTACTTTAAACTCTGAATTAAAGAAACATAATATTGAAGATAAAAGTTCAAGCAACCATTCAGAAGATTCTAATGATACAAAAAATTCAGATGAAGACAGCAACAAAGAAGACAATCAATAAAATAAAAAAGTGGAATTCCACTTTTTTATATATCTCCGCTTTCTCGCAACATCTTTTCAAGGCCGTTAACAAGTTTCTTAGAAAAAACTCCTGTTCCTTTTTGCTTTTGTTCATATAAAAAATCTAATTCTTCATACAATTTATAAATTAATTTCAATGAATAATTGTATTGACTAATTGAATTTTTAAAATCTTGCCAATCTATAACGCTAAAATCTTTATCATCATAACTTTTAATATCGATATCAAAATCAATGTATTTAATTACTTCATTTTCAATATAAAAAGGGCTTGCTAAATTAATATAAATATAGTTTTCTTTAGATTTATTTAATGTAATAGAAGCATTATAAAATTTGTTTCTAGCAAAGAAAAACAAAATAGGTTCAGAAACAACTCAATTGATAGATTTTTCAGAAACTTTTGTTTTCATTAACAAAACAACTACAAAATCAGTTAAATTTGCAACTATTTTTGCCCCTTCATATTGTCTGTATAAAGTTCCATCATATTTAAAAACTTGAATGGTTGTTAGTTGTCCCATCTTAAAAAACTTTTTATGTTTTTTTATATTTTTTGAGTTTGGTGTATCAATTGTTTTTAATAAATCATCAAAACTCTTTATACTTTCTTCTTCCATAATACTATTTAAATGGTATCAAATAAAAAACAAAAAGATTAATTAATTAAATTTTATTTTTAATATATTACAAATTTAACAACTTGCGATTTGCTGTATATGTTATAATAATTACGTGATTTAAACAAATTAAGCATTTTTAGAATAAATCTATGTATGCTTTGAAAAGTAACCTTTGGTTGCTTTTTTTGTTTATTTAGTGTATTATAATTAATATGCGACTAAGATTTAATAAAAATGCAGAAACATCCTTAATGGCAAGCCCAATGACATTTAAGGATTTTCCTATTGATAATAAAAAGAATACTATATTAGAGATTGGTATGGGGAGAGGAACCATGCTTACAAAATTAGCCCTAAAGCATCCTAACATAGAATATATTGGACTTGAAAAATACAGCACGCCAGCTTATTCTGCTTTAAAAAAGGCAATTGACCTCAAACTTGAAAATTTTCACATAATTATAGGAGATGCAATAAATTTATCAACTTATTTTAAAAACAAAATTAAAACTATTTGATTAACATTTTCAGATCCTTGACCTAAAAAAAGACATTATAAAAGAAGGCTAGTTTATAGAGATTTTTTAAAAATTTATCAAAACATTTTGGATAAAGACGGAGTGGTTTATTTTAAAACCGATAATGATATGCTTTATCAATTTGCAATAGATGAATTAAAAGAAATTAATGCAAAAATCATTTATCAAACGTCTGATTTACACCATTGCAATTTCGAAATAGAAAATGTTTTTACTGATTATGAAGAAAAATTTAACAAATTAAACAAAAATATAAACTTTATTGCATTTACTTTCAATTAAAAAGGCCTGTAACAAAGGCCTTTTTTGTTATAATTATATAATAATATAATTATATTGTGTAAATGGAGTAAATTATGCTTTTTGGCGAAGATGATAACAATAAAAAAGAAAATAAAAATTTTGAAGAAGATAACAGCCACGATTTAGATGAATATACAAATTTTGATGATAGAATCAAAAAAATCTTTAGTGACGATGATGAAACAAAAGCAAAAAAAATAGATGAAGAAGACGAAGAAGAAATTCCACAAGACAAAGAAGGCTATAAAGTAGAACCTCAACTTTTAGATAAAGAAATTAATGGTTTAAAACCTGCCAATTTATCAAAAGTAATGAAAACATCATTCATTGAATATGCAATGAGTGTCATAGTTTCTCGTGCTCTCCCTGATGCTCGTGACGGTCTAAAGCCAGTACACCGTAGAATTTTATATGGCATGAGCGAACTTGGAATGTTTTATACAGCGCCACATAAAAAATCGGCAAGAATCGTCGGAGATGTTTTAGGTAAATATCACCCACATGGAGATTCATCAGTATATGAAGCTATGGTAAGAATGGCTCAAGATTTTTCACTTCGCTATCCATTAATTGATGGACATGGTAACTTTGGTTCTGTTGACGGAGATGAAGCTGCCGCAATGCGTTATACAGAAGCTAGAATGAGCAAGATTGCTGGCGCAATGGTTGATGGTATTAAAAAAAATACTGTTGACTTTATGGATAACTATGACGCAACTGAAAAAGAACCAGTTGTTTTGCCATCACGTTTCCCTAATTTATTAGTTTCTGGTTCAAGTGGTATTGCTGTTGGTATGGCAACAAATATTCCTCCTCATAACCTAGGTGAAACTATTGATGCAGTTTGTGCACTTGCAAAAAATCCAGAAATAAATGTTTTTGAATTGATGGAATATATTCAAGCTCCTGATTTTCCAACAGGTGGAATAATATTTAATAAAAAAGGATTAATTGAAGCTTATAGCACAGGTAGAGGATCAATCACCATTCGTTCAAAAGCACACATACAAGAACTTTCAAACGGAAAAAGTAAAATAATTGTTACTGAAATTCCATACGAAGTTAAAAAAACAGAAATCATGGAAAAAATTTCTGAACTTATTAAAGATAAAAAAATTGATGGAATTGCTGATTTTAGAGATGAATCAAATAGAGATGGTATTAGAATTGTAATTGATATCAAAAAATCTTTTGTTCCCGAAATTGTTTTAAATAACTTGTTCAAATTAACAAAATTACAAAGCAATTATTCATTCAATATGATTGCGTTGGTAAATAATGAACCAAAATTGTTGAACCTTAAGGCTTGCTTAGAAGTTTATTTAAAACACCAAATTGAAGTTGTAAGTAGAAGGTTAAAATTTGATCTTGAAAAAGATAGAGCAAGAGCTCATATTTTAGAAGGTTTAAAAATCTGCATTGAAAATATTGATGCCGTAATTAAAATAATTAAAGAATCAAAAACTGACCAAGAAGCACAAAGCAAACTTTCTCAAGCATTTAATCTAAGTGAAATACAAACAAAAGCAATTGTTGATATGAAACTAGGAAGACTAACCGGATTAGCAATTGAAAAAATGAATGAAGAACTAAATGAAGTGCATGAAAGAATAGCTAACTACATTAAGATATTAGGCGACCATAACTTACTAATCAATTTAATAATCGAAGAATTGCAAGAAATTAAAAATGCTTATGGCGATGCTCGTAGAAGCGATATAAATTGAGATGAATTGAGTAGCATCGACAATGAAGATTTGATTCCAAAACGTGATATTGTTATAACCTTGACAAATAATGGCTATATTAAAAGACTTGATATTGAGGAATATAGAGAACAAAGAAGAGGCGGAGTGGGTGTATCAACTACAAAAACATATAAAGATGATGCAATTCAAGATATATTAGTTGCAAATACCCACACGGACTTGTTGATATTTACAGATCTTGCCAAGGTTTATAGAATTAGAGGCCACGAAGTTCCCGTTGGCACCAAGCAATCAAAAGGTATCCCTATTGTTAATGTAATTCCTTCTATTCAAAAAAATGAAAAAGTTATTAAGATTTTACCAATAAATGAATACAAACAAAATGAATATTTACTAACAGCAACTAAGAAGGGGATTGTTAAAAAAACTCCAACTATTTTATTTGAAAGTATTAACAGAAACGGAAAAATTGCCTTTGCCATTCGTGATGATGATGACAAATTAATTGATGCATTGATTGTAAAAGATGAGGATGAAATCTTTATTGCTGCCTCAAATAATAAAATTTGCCGTTTTGATTCAAACGAAATCAGATCAATGGGAAGAATGGCCTATGGTGTTAAGGGAATTAAAATTGACGATGTTGAAGACAATGTTGTTTCTGTATCTTCTTCAAACCAAGGAAAATATATATTTAGTCTTGGGAAAGAAGGATATGGAAAATTGACACTTGCTGCAATGTTTAGAAAAACAAGACGTGGGGCAAGTGGGGTTTATTCATTAAATGAAGAAAAATCTGGTAAATTAATTTATGCTGCAACTGTTCACGGAGTTGAAGATCTGATAGTTTTAACAAGCGATGGTGTTGCAATTAGATTTTCTTTAAAAGATGTTGCAATAACCGGTAGAAATACTAAAGGTGTTAAATTAGTAAATCTAAAAGGAAGAACCACAAGCATTGTTGCAGCAACAAAAATTTTAGATGATGATTTAGAAGACAATGACAGAAAATTATCAGAAAATGAATTCAAAGAAATTGAATCAGATGCTAAAAATAACCTTAATCAAAAAAATGAATAATTATTAATAACAAATAAACTATAATTATTATGTATGCGTTAAAATGCATACAGATTGTAGTTGCACAGAGAGGTTTTGAAAAATAATCTCTCTTTTTTATATTTTAAAAACCACCTTATAAAGGCGGTTTTTAAAATTTAGATATATGTTTAATTTTCTGGGTTTTGTTTTAATTCTGTAAGAAATTTCTCTAATTCTTTGTTTAAATCAAGTGATTTCAATAGTCAAGGAGTTTTATTAACTTCCTTAAAATATTCAAACATTCCTTCAGGTGCATCTTTAAGTCCAAATCATTCAACTCATTTATTGATAGATTCTAATTGATTTGAATTGTTTGGTAATTTTAACGTTAATTCGTCTAATTTAGTTTTTCATTCAGAAATTTTTGAGGCATATTTTCCACTTTGTTCTAAGCGTTTTATAGTATCATTAACTTCTTTGTATATTGATTTGTTTGGATCAATTCTGGTATATGGTATAGAATAAGATCCACGTTTAAGGGTCGCTGTTGTGGCTTTGTGTTCTTTAAAGTTTGACAAAATCTTTTTTCAAACAACATCGCAATAAAATCATAATCTTTTTTCAACTTCTGACATTGTTTTATTAAAGTTAGATATATATTCATCAAAGGTCATATTTTTAAATAAGATTTTCTTTTCTTTTGTTTCAACTACAGTGTTTTTAGATTTGTCAATTTCGTATTGGGATTGTTCAAATTTAACAGCATTTTTAGCAAATTTATTATTTCATTCGGATGAGACCCCTTCAAAATTAAGTCCATCTATTTCTTTTAAAACCTCACCACGAATATATGAAGCAGTATTTCAATCGTGAAATAATCAATTAATCCTATCAGTATTTATATTTCCATCAAATATTTTTATTAAATCTTTAACAAATTTTTCTTGGATTTCTTGTATATCATTTTCATATTTTGACGCTTCTGCAAGAGTTGCCAATTGACTAGAATTATTTTTAATATTTGTTAATTCTTGCAATGCCTTTTGTTTTATTTCATTTAGTTGTTGTGTTACACCTAAAAAATCTGGATCTTTTACACTACTATCTAGCTTATTAAATTTTTGTAAACTATTATTTACATCATCTAGACTATTATTTATTAATAATTTTTTAGCCTCAAGATATGCCACTGAAATTTCATTAATTACTTGTATTATTTCTTCTCTTGTATGTAGAGTACTAATATTATGTTTCCTAATAGACCATTCTATTCTTTCAATGTATGCATTGTATTTATCTCTAGGGCGCATTTTATTTTCAATATATTCTTTTGCTTCTGTAATTAAAGAAATTAAGCTATCCCTTAATTTTTGAATATCATCTTCAACAAATTTTTTACTTTGTTTTAAAATTTCGTTCAAGTGAATATTTGTAGCTACTATTTCTGATTTATTAGAACTAATTGAAACGTTTCCTCGTCTTGCAACTTCTCTATTAAGATTTCTAACTATATTTGCATAATAATCAGATTTAACAACATCTTTGGCCAAAAATTCTTCAACTAATTTTCTAGTTTTTTGTAATTTTTCAAATTCTTGTTCTTTCGATTGCTTAAATTTGATCAATATATCTTCAACTTCAGAAATTTTAAAAGTTAATGATTTATTTAAATTATTTATTTCGTCTATATTATTTTTGTTATAAACCTCTAATGCATTAATAATTTGTGAATTTAAATCTTCCTTAGTTTTTTGAATATCATCATCATTATCAATTAATTTATTGTCCAATATCCTCATAGCATGAATAATATTTTCCAACTGTTTCTTTGCTGCAAAAAGTAGTTTTTGCATGCTTTGCTTTTGAATTTTTGCATTTAATGAATCAATTGAACTTTTAATCTTAAAAATTTCTTGATGAATATTATCAATTGGCGATTCGATATTGATCATTTTGGAATTATCAAAGACATTTTGTTCTTCAATTGAATAAATTCCTTGAATGTTTTTATTTTCTAGCAATGATTGCAATTCTTCTTGAAGAATTTTAAATTTTTTAAAGAGCGATTCTTTTTGTGTCTTTTTATCCACTTTATTTTGGCACGATCTTATTGCAACGGTAGCGATGGCAGCTATTGATAATCCGCCACAAAAAATTGCTAATGGAAGAACAATTTTTCTACTTTTTTCTTTCATGTTTCTCCTCCATTTTTTATTATTTTTAATATTTGTAAAATTATTTTATGTTATTTAGTGCATAAGAATATTTTTATATAAAAATAATAGGGATGAGATCATATAAATAGAAGAAAGGGTTTATTTTTTAAATATATATATAGATTAAGCAAATAATTTATTATTGATTAATATGAATATATTTTACTTGTGTAAAATAATATTTTTTGTCTAATCATAATTATTATTGGATAAAGTACAAAAATAACTGCTGTATATATTGGAATTTCAATAAGAGTTTTTGCTATTATTGGAATCATAAATATTGGGTAATATGTTTTGTAATCTCAATTACCTGATCTAAATCTATTATGATAATTTATGTATGCAAATGGTCCTCAAAATCATCTACACAACATTAAAGTGAAAAACACAACCAATAAAATGCTAAATAAAAGTACAACATTGCTTTTTACAGAAAATTTTCTATTTTTTATAAATAAAGTTAAAAAAGTTATAGAAGATATTCAAATAAATGCCAATCCTACAATAGCAATAGACAAAACAATTCTGATTGGTATTAATTTGGTGCGTTTTACTGCTGTTTCATTTATTGGCAAATTGTTACCGTGAATTGCTAAAACTATTACAAAAATTGCTGTTATTATGCTTAAAAAACTAAATCCAATTATTCATGTAATCTTTTGTTTAGCATACATTAATCTTAATAATCAGCCACTTATTAAAGCAATAAAAACAGGCACTAACGCATATTCAATCATTCATGTACTTATTCCTCTAATAACCTGGTTTAGCGTATCACATAATATACCTAAAAAGGCACCCTTTCACGGTCCTAATGCCAACCCAAAAATAATGAATACTGCATGAGTAATATTAATATTCATTATTCCTTTGAAAACAAATCTTTCTAAGATTGCAGCAATTATATAAATAGCTAAAAGTAAAGCAAATAAAGAAATTTCAAAAACTGTTAATTTATGTTGTTTTGTTTTTTTAATACGTCTAATTTTTTGAATAAATTCAAACATGTTTCTCCTAAAATAAACCCTCAAAGGGTTTTAAATTTTGATAAATTATATTTTTAAATACGAGTTTATATATACGAGAACAGTTTTTTAAATCATAACTATTAAATTTTAACTATTTTTTAAAAAAATAAACAAAAATAAAAATTTCGGTTCTTTGTCCGAAATTTTTAAGCCTTATTTCAACACTAATTCTGTCAAAGTTTTTGTCATTGCAAACATTGGTTTTTCATCAATATCATTAGTTCCTGCAACATCTAAGTGAATATATTCAATTCCTTTTGTAAATTCTTTTAAGAACATTGCTGCTGAACAAGAACCTGCATGTCCGGTATAGTCTGTATTCTTCAAATCTGCAACAATTGAACCCTTCATAAATTCTTCATAGTCAGAATCAAAAGGCATTCTTCATATCAACTCATGTTGAATGGTTGCTGCATCGCTAATTTCTTTTCATGCTTTTTCAGTTGTTGATCAAATGCCTGTATATGTGTCTCCTAGGGCTGCAACCATTGCACCAGTTAATGTGGCAACATCAATTAATCTTGTTGCTTTTAATACATCAGCTCCATAGTACAAGCCATCAGCCATAACTAATCTACCTTCAGCGTCAGTATTATTTATTTCAACAGTCTTTCCGCTCATTGAGGTCCAAACTGAATCAGGCAACGATGCATCTCCATTAACTCTATTGTCAGTAATGCACATAATTGCTGATACATTTGTTTTTGGTTTTAATTGGGCAACAGATTTCAAAACGGATGCAACTATTGCAGAACCTGACATATCATATTTCATTCCTAACATGTTGCGGCCTGTTTTAATGTTGTAACCGCCAGAGTCAAAGGTAATTCCTTTACCAATTAAAACTGTTTTTTGATTTGAATCAGGATTTCCTTTATATTCGATTATTACAACTCTAGGTTCATAAACTGAACCTCTATTCACAGATAGTAATAATCCCATACCCAATTTTTCAATTTCTTTCTTTTCAAGAACTTTAACTGATAGATTTTTATATTCTTTAAAGTCTTCAACAACTATTTTAGCTAATTGTTCAGAATTTAATTCATTAGGAGGGGTAATACCCAAATTTCTTGCTCAGTTTTGAGATTCAATTAATATTTTTGCTTTATTGAATGAATCAATTACTGATTGAGACGCTTGTTCTATAAACAATTCTAAGTTGTTTGGATTTTCTTTATATGTAAATGTCTTTTTATTGTATAACTTTGCAGCTGAGAAATTTATTCCTTTTGTGAATGCATCAACTACATCATTAATTGTTAAATTTTCTGTTACAAAAGAAGAAAGATCAATTTGATAATCGTAGGCAGCATTCAATGCTAAATCTTGTGCCAATGAATATAAAGAATTGTATTGTAATTCTTGCTTTTTGCCTAGATAAATATACGAAACTTTTTTATCAATAAAATCTGTTATTGCTTCTGTTTCTTTAACTAAAAATTCTGGCAAAATTTCATCTTCAAATATTGCTTTCAATAACAAATGATCATTGCGTTTTGTTGATAATTGTTTAATTAATTGCATACTATTCTCCTTATTATTTTTGATTTGCGGCTAATTCAACAAAGGTAGAAACTAAAATTCCTAGTCCTACTCCTTTATTGTCAGCGGTTCCTGCAATATCGCAATGAATATAGTCAACATTATTTGTAAAATGTTTTAAGAACATTGCTGCTGTATTACAATCTGATTTTTCACTGTTGTTGTAATTATTAAGATCAGCTACTTTAGATTCTTTATTTGGTTTATCATAATCATCATGTAGAGGCATTCTTCAAACCTTTTCATGAGCAATTTTTGCCGCTTTGTCGAAATCTTCTCATCTTGGTTCAGAAGTTGATCAAATTCCTGAATATGTCTTTCCTAATGCTCTTTCCATTGAACCGGTTAAAGTTGCAGCGTCAATTAATAATGAAGCATTCAATTTCGAAGCACCGTAGTATAAGCCATCAGCCAACACTAATCTTCCTTCAGCATCAGTATCAGTAATTTCAACTGTTAAACCACTCATTGATTTAATTACTGATTCAGGCATTGTTGGGTGTGTATCTATTGCGTTATCTGTAATCATCATTATTGCTGCTACGTTTGCTTTTAATTTTAGTTGAGCAATTGCTTTAACTGCATATGCAACAATAACAGAGCCAGACATGTCAAATTTCATACCATCCATGTAGTATCCTTTGGTGTTATATCCACCAGTGTCAAATGTAATTCCTTTTCCTACATAAACATATTTTTCTTTACTTTCGGGGTTGCCATTATATTCAGCAATCAAAACTCTTGGTTCATAAGAAGAACCCGAATTTACTGCCAACATAAGTCCCATGTTATTTTTCTTTATGTTATCACGATCTAAAACTTTTATTGATAGTCCAGGAATGTTTTCTAAATCTTCTTTTACTACCTTAGCGATATATTCACTAGTTGCAACATTAGGGGGTGTCATTTGCAAACTACGGGCTTTATTTGTTGCTTCAAGAATTATTGACAATCTTTCAACTAATTCTTCATACGATTTATCAGCATAAAACAATGTTATTGTCTTTTCTTGTTTGTTTTCCTTGGTTTTTGCTGAATAAAGATTTGCATTTTTAAATGCTAATTTAGATACAAATGCTCTAAAAACTTCTTCTGTTGACACAACATTTTCTTTAACAAAAGAAGCAATATTAATTTGATAATTTCTTCTATTTGAACTTAGAATTTTGTCAACTACTTTTAAAAAACTAAAGTAGTTATCTACAGTTTTTGATATGTAAATAAATGCTTCATTTTTCTTAAAATCTTCTGTAATATGATTATCTTTCTTGGCAATATTTAAACTAGCGTTTGAGCCTTCATAAACTGCTGTTAAAAGCATATCTTTATTACGTTTGGTTTCATAATAAATAGAATTCATTTTATTCCTTTCTAATTTTTTAAATAAATATCTTTTAGATAATTAAATTATAATGATTAATATTTTTTAAAACGTAAAAACATAAAATAAAGAGAAAAATTTTAATATTTTTGATTAAATTAAAAATAATAAAAAAATTAGCCTTGCTAAAAGCTAATTTTGTTATTTAATATTTTTAAGTTGATTAATAACTAAATCTATACTGAATAAGATTGCGTCGCATTGTTGATCACTTAATTTTGCAAGTTCATCATCGTTTATCATGTTTTCATATTTGCTCATGATTTCACCGGCAGATGGTATATTTTTTGCCATATCAAGTAATTGCTTTATTTTTGATTTAACTTGTTTTTGCTTTGATGTCAATTTTGAACATGATATTGCTACTAATGGTAATGAAATTAGAGAACTTGCTCCTAAAATAGAAAAAATTATTTTCTTGCTCTTCTTCATAGAATGTCCTTTATCTTTGTTTAATGAATTGTTAGATATTATAATTATAAATATAAATTTAATTATTTTTTAGTAGATCAATCGATTTCATCTTTTGATAATGATTTAAGCTTAAAATTTATTTTTTTAAAAATATTTGAACCAAAAAATCCTTTATATGCAGCAAGAGGTGAAGGATGTGGTGCATAAACTATTATTTGTTTAGACATATCAATGTTTTTTATAAAATCTCTGGCGTTATTCCCTAAAATTAAATAAATAATATTGCTATAACACGAATTAATTTCTGTTAATAAATTTTGATTGAATAGTTCTCAACCTAAATTCTTATGTGCCAATGTCCTTCCTTCAATGTTTGTTAAAATTGAATTTAAAAGTAAAACGCCTTGGTTTTTTCAATAATTTAGTGAAAAAGTTTCAAACGTTGAATTTGGATATTCGTTTTTTATTTCAACAAATATATTCTTCAAAGATGCCGGGCATTTTGTTGCATTGCTTGAAAAACACATACCATCTGCATCGCCTTTGTTAGGATAAGGATCTTGACCAATGATAATAACCTTTAGGTTGCCAAAATCAAAATTTTTAAATGCAAAAAATACTCTATTTGGCTTTGGCGTTACATTAGGCAACATTGTTAATTTTTTTAAGACTTCAAAATATGGTTTTGAAGTTTCTTTTTTTAAAAATTCTAAAAAAGCAAATTTTGACATTTTTAAACTCTTACTCTTTTTTTCCCTTCAAATTGATATTCTATTAAATATAAATATCCATCATTAAATTTTATTTTTAATGGAGTACTAATTTTTGTTGTTGAAGCCCGGAAGATTTTTAATCTTTTATTTTGATAAATTAAATAAGCACCTGGTTCGTCATTAAAAGCTCTGATTTTGTTTATAACACTTGAAAGATTTTCGCTTTGATCAATTCTTTCCATACCATTTAATATCTTAGGAGCAAATGTTACTTTAGATTCATCCTGAGCCACTCGATTATATTGGCCGTTGTAAATTTCAAACAATCAATTACCAAGATTTTTTGAGGCTGCTTCTGCTAATTTATCAAATACTTCAATTGCTGTATCAATAGAATCAATTTTTACTTTTGAGGATTTTAGGATATCCCCCGCATCCATTTGTTTTACCATTTCAATTAATGTTATTCCTGTTTCAATATCACCATTGAGTAATGCATATTGCACAGGCGCAGCCCCACGATATTTTTCAAGCAAACTTCCGTGAACGTTCAAGGGCATTTTTTTAGGTAATTTTAATATTGAATCAGGTATAAATTGTCCGAATGAAGCCGTAATAAAAAAGTCAAAATCCATTGCACTAAGTTCAGGCAAAATTTCTTTTATTTTTTGAGGTTGAAAAACTTTTATATCATACTGTTTGGCAAGTTTAAAAATTTCTGTTTCAATTACATTTTTGTTTCGGTCTAGTTTCCTATTTGGTTGAGAAATAAATGCCAAAACATTGAACCTTTCATCATTTAAAAGAGGTTCAAAAGTTTTAGCGGAAAATTGTCCAGTTCCCGCTAAAACTAAATTAATTTTGTTATTTATATTTTTCATATCAAAATTATAAAATAAATTATTTTTTATAAGCTTATTATCTTGTATTTCAAGCAAATGTTTTTATTGACAATAAACTCAATATTACAATTCAAATTCAAGGCAATATGAAGTTCAATAAATTTTCTGTTTTTGAACCTATTTTTGTTAAACTATCATTGCTTAAAACGTTATTTGAAAATTGAATGTAACTACTGTCTAAAAATTTAAAATAGTTTGCAAATTCATTATTCTTTAACAATTGGTCTAAACCATTTTGATTGAATGCTTCAGCAATGGTTGAATATTTTAAAATTACAGGTTGATAAACATTTAAAATATTTAGTGTCTTATATTCGTTGTTAATATTAAATATGTTATATTGACTATATTTTATTCCGATTGGTGTATTGGCTGGCAATGCATTATTATTTGCTAGAAATAAATCTTTTTGTTGATTCAAGAATTTCAAAATTGTGAACCTTGCAATTAGATCTTGTTTGCTATTTGCTTCTTTCAATAAATTTGCATATTTTGTAACACCTTCAATATATTTTTGAAGTCCTTCAATTGTATAGATTTTATTGCTTGAATCAGCACTTGAAAAAGGTTTTAATAAATCTATATTTTTTATTTCATTTCCGATGTTAAGAGTTGCCTTTATTGAAGCAATATTATACTCATTTGATAAGGATGCTTGTCCTTGAAAAGAAATTGTGTTTTGAATTATTGCACTCTTAAAGGGCGTTAAGTAGGAAACGTATCACATTCCTTTTATATTTGAAATTTGAGAAATAGGCAACACTTGACCCGATAAGAACATTGTGATTATTAATAGCGAGCAACCGATTGCTTGAATAGTTAAGACTGATTTTGAAACGCTTATAATGAACAATCCAACAGATATACTTACAAAAATTATAATGACAAAGCTAAATATGTAACCAGGCCAAAATATCCTTGAAAAAATTTCTTTAATACTACTTCCATATAAAGTTATATTTAGACTTGCTTCTCCAATTTTTATATTATTAATTGTTCCTAAAGAACGACCTGTTTTAAAATATTCGCAAAAAAACATTAAGGCAACCAGCATTGTTCAAAGACCAGAACAAATCATTATAATTAAATAAAATATTGCTGTATATGCCAAGAACGATTTAGGCCTAATGCTAGAAGCACCAATTCTTTTTAAAATTGTTGATTTTTTAAACTCAAATATTGCCGTTGGCAGTGAAATACAGGCTATTGCTACCGGCCCAATGGTTATTGTGCTTCCTAAAATCATTTCATAACCAAATATCGTTCCTAAAATAGCAACGAAAATAATTGGATAACCGAATGCAAAAAATGGTCCTACAAATGATTTTCAAAAATGTTTATTAATAATTGCTAATATTCCCAAAAACTTATTTTTGTTAGCCTCAGTATTATTGTTTTTTTTAATAATTTCTTTATTTTTCATAATTTTAAATATATTCCTTAAGACATTGAGATACTGATCCATATTTTTCAATTATTTCGCTTTTCAAAGCATCAAGGACAATTCTGCCTTTATTCATAATAATAATTCTATCTGCCAAAATTTCAATTTCATTTATATCATGAGATATTAAAATCAAAGTACAATCAATTTCTTTGATATATTCATTCAAAAATGATACTAATCTTGTTTTTATTTTTATGTCAAGGCCTGTGCTTAATTCATCTAATATTAACAACTTTGGTTTGTTAATTATTGCAAGCAATGCATTAAGTCTTTGATGTTGACCACCACTTAAAGAAGAAGCTTTATTATTTATAAATTCATCAACCCCAAAAATCTTTATAAGTTTTTGTAATTCTTCTGCTGAACTTTTATTGCCATAGATTTTATTCATTGAATAAATAACGTCGTTAACCGATAAGCCTTGAGGATAAGAAGAATCTTGAAATTGAATTCCAATATTTGTTTGTGCACTTTCGTTATGGTTTAATCCGTCATTAAAATAATATTCAATGTTACCAGAAGTTGGTTTTAAAATTCCTATAAACATTTCAACTGTTGTAGTTTTTCCTGCTCCATTTCCGCCTAATAGAGCAATGTTTTGATTCTTATAAATTTCTAGATTTAAATTATCTACAACTTTTATTTTTTCCTTTTTTATTTTGAATTCTTTTGTCAAATTTTTAATTTTTACCAACTTTTTTAAATTGTATTGGCGCATTTCAATTGAATTTGCTTTAATTGTGTTTTTTTCGTGATCTTCTAAATTACTTAAAAAATTGTCTATTACTTCTAGACTTTTATTTTTGCAAGCTTTTTCATTTTCCACTATCTTAACTCCTTATTTTATATATTTCCCCTTTTGCCCTAATTATATCAATAACTTAATAAATAAAAACAAAAACCCGTAATTAAATAATTACGGGCCAATGTTTTTATGCTTTTTTTGTTTTGCTAAAAATTTTTGGTTCTGTATGTTTGAATATTCGTTTAAAATTGGGTATATGTTTAACAACTATTATGAGCGAAGCTAATATTAAAATAATTACATGTGCGGGATATACGACATTTTGTTGCATATAGCCGAATATTGTTCCTGCATAAAATATATAAATTAAACCTAAATAAGGAATTATTCCACAGCCTATTGCCGAAGCAAGTGAAACATATTTTGTTGAAAAAACTAGTATTACATATATTGCTACAAAAATTAAAAATAATAGAAAATTATATGCAAGCATTATTCCAAAAAAGCAAGCTACTCCTTTGCCACCTTTAAATTTAAAATAAATTGGAAAGACGTGCCCAATTACTGCACCAAGCCCTGCAAGCATCGGAAAAATATATTCAAGATTTTTTAGATATTTTTTAATTAAAAATGCAATTATTGTTGCTAAAAAGGCCTTAAAAATGTCAAAAGCAAAAACTAATGCTGCAAATTTTATTCCAAAATTTCTTAATGCATTTGTTGCTCCAGGATTTTGCGAGCCAATTTTTCTTATATCATTTCCTCTTAATTTGGTTATTATTATTGCCAAATTAAAAGAGCCAATTAAATATCCTAATATTAAAAATATTATATTTAATCACACATATTCACCATAAAACATAAATAAATGATACACACAAGAAAAATTATTTTATAAAAACTTACAAAAAAATAGCAAAACTCTATTTTTGTTTGCTATTTTTCGGTCTTTGTTTCAATGTTAATTATATTGCTTGCAATAACATCACTACCAACTAAAATATCATAATGAGCAGATATCTTGTTTCCTTCAATAAAAACATCTTTTAAATAAAAATCTAGAGAAAATTCTTGAATTTTATTGCTTACTCTTTTGTTTTTTTGTAACTCGATATATGAACCAGAATATTTTATTTTTAAAGTATCGCTATTGACTAATAAATCACATTCCTGATTATTATTATCAATAAAATTTATATGAATAAATTCATCAACAACTTCTTCGGTATATGGAACAAATTCTGTTTCTATCGTTTTAGAGGGTTCATTATTGAATTCGTTTTTTGTAATTAAACTATATTTTAATAATTCCATTTAATCTCTTAAAAACTAATTCATCCCCAAAAAGATAGATTGCCTTTGCAAGTTCAGGGCCATGTTCCTCAAATGTTGTTGCTTTTCTAATTGGCATGAATAAATCTCTTCCTTTAACATTCAAGATAGTTTTTGTTTCATCAATTGCTTTTTGTATATTTTCTATATTGAATTCTCTTGAAATTAATTCTTTATAAAAAACTTTTACAACATCTAATCTTTCTAACTCTAAGTCGCAATTTTCTTTTGGGTTTAAATAAATTTCTAAATCTTTCTTTAATGTTGAAATCGTTGGACTTGATTGTTTATAAGTTTCAACAAATAATTCATTTCATTCATGTTCTTTTGGAGAGTTTATTTTTTTAATTAACTCTTCATTAGAAATATTTTTCATATATTGCTTTGCAAATCAATCCATTTTTATAATGTCAAATTTAGATGGTGATTTGCTCAATCTTTCTGGATCAAATTTTGCAATTAATTCTTCTCTATTCATTAATTCTTGCTTGTCTCCAGAAGTTCAACCTAACAAGGCTAAAAAATTCATTATTGCTGCCGCAGGATAGCCTTCATTTTTATAGTCTTCAATAAATTGTTTTAAAGTTTTATCTCTTTTTGAAAGCTTTTTTCCTTCCATATTCGTAATAATAGTTAAATGTCCAAATAATGGGGGTTCTCATCCAAATGCATCGTATATTGCTAATTGCTTTGGTGTGTTTGTGATGTGTTCTTCGCCTCTTAGTACATGTGAAATTTCCATTTGGTGGTCATCAACAACAACTGCAAAATTATATGTTGGATAACCATCGCTTTTCATAATAACAAAATCCCCTATATCATCACTATTAACTGAGATTTTCCCTCTTACTAAGTCATTTCAAGCATAATCTTTATTTGCTGGCAATTTTAATCTTATTGAATATTCTTTAGCTTCTTCTCTTCTTTGCTTTTCTTCATTAGAAATTTGCAATCAATTTAGATTATAACGAAATGACGCAACGCCCGCTTCCTCTGATTGTTTATGTTGTATTGCTAATTCTTCCTCTGAATCGTATGCTTTATAAGCGTGTCCATTTTGAATTAGTTCTTCAACAAGTTTTTGATAAACTGGTAATTTTTCGCTTTGACGATATGCGCCATATTTTGAATTAGGTTTTAAAGGACTTTCATCAGGGATGATACCAAGTCACGCTAAATTATTTAATTGACTTTCTTCACCTCCGACAACGTTTCTAGCAACGTCAGTATCTTCTAGCCTAAAAACAAAAGTACCATTGAAATGTTTTGCAAATAAATAGTTAAATAATGCAGTTCTTGCACCACCAATGTGCAAATACCCAGTTGGGCTCGGTGCATATCTTGTTCTTATAGTTTTCATAATCTACCTTCTTTTATGATTTTTCAAAAATAGCAGTTATGGGTTCTGAAGCATTGCTTCAAAACGCATCCTTATTTCCCTTCATAATTCCTTTATAAAAAGGTCAAGGTGCTTCCTCGATAGAAGTATTAACATTTCTATTTTTTCCTTTAGCTAACAATGATTCAATACTTGGATCTAGGAAATCTGATTTTCAATTAGGGGCTGCCTCGTTTTGTTTTAAAGTTCAATATAAAACTAAAAATAATGCTCTTGGATTATCTCGATAAGGTCAGTATCTGGAATCATTTTTTGTAGCAGGAAAATAATTAATTAATTCCTCTCATGTAAAAGAGAAAACTCTAAAATCGTAATTTGGAGAAATATTATTAAAATCTAAAAATCTGCTAAAAAATTCTCTATAAACTCTTTGCACTGGTTTTCCATCAATTACTTCGAGTTCATTATTCAATACTTCTTTCAATCTTGGGTATCCATTTTCTTTGTCATAAATGTAGTTTCCATTAGCATCTCTATCGAATTCTATTTCACCATTTTCCAATTTTTTACCCTTAAATTTTAATAAATTTCCATCGGCATCTCTAAAGTAAATTGGATTTCCTTTTTCATCTTTTAAATTTTTTGTTAATTTGTACAAGGAATCTCTTGGCGATATCGGGTTACTTACTGAATAATTATTTTTATTAAAATCAAGTGCAGAACCCTTAACGTGATAACCATATTTTTCAAATGATTCTTTCGCTTTTGAATTGAGAGTTATGGTTGCTGTTTTGTTACAACTTGCCGCAACTAGTGGCAATGCAAACATAGGAGCGACACTTCCTATACAAAGTAAAAATTTTGTCATAATTGCTTGTTTTTTCATGTTTAAAATTATACAAGTTTTTTTAATATATTAATAACCATAAAAATAAAGTTTCTCTAACTTTTCGCCATTTCAAAAAAATAAACTTTTTCAATCAATAATTTAAATATGTGAATAAATAAACAAGACAGCGTTAAAGATTGCGCAATTTATACATTGCAAGCAATGATTTGCCACTATTTTAAATATAAGGCAGATATAAATTTTTTAAAGAAAAACACGATATACGACGAAGAAGGAGTGTCGCTATATTCATTTAAGAAAATTTCTGATTTTGTGGGGTTTGATACTAATATTTACAAAGTAGATTTTGTTAGTTTTATTTCAAATTTGAATGATGTTTCATTTCCGTTTGTCACAATATTGAAAAATGATAATTTCTTGCATTATACAATTGTTAAAAAAATTGATAAAGCCTTTGTTTATCTATGTGATTCTGAATTTTTGAATTATAGATTAGAAATTAATGAATTTAAAAATAAATTCATAAATGTTGTAGTTATATTAAGCAAAAAATTTAAAACCCCAAATTGCACAAAAATTAACTATAATCTATATCTAACACAAAACACAAAATTAACAATAATTAGTATATTTTTGGCAATTTTATTCAATGCTCTTATGCTTTCTTCTTCATTTTACATTAAATATGTTTTAAGCATTTTATGATCGGAAAACGCTAATAATAAATTGCTGTCAATATTAATTATTTTTAGTTGAATTGCAATATTAAAGGTAATTACTTGATTATTCAAAAAGATTATTATTAATAAACTAACTTTTTATTATGAAAAAAAGTTATATTTGTTAGTCAAAGAAAGATTAAAATTTGCAAGTCAAAAAGACCTGCAAAAAATTAACCAAAATGATTTTATGGCAAGAATATTAAGTATTTCAACTATTGCAGAATATAAAGCGGGTTTATTTTTGTTTTTATTTGAAAATATAATTATTTTTATTAGTTCAACATTGCTTTTAGGTCTGTTTAACTATAGAATATTATTAATTTGTTTATTAGGCATTTCAATAACCTTTGCAATCTCGTTATTCAGTTTAAAATATCAATCAAAAAAAGCAACAGAATTAATAAATTTAAAGATAAAATCAATTAAAATATTCAATAATTATTTTTTAAATTATGAATATAGGACGAACGATTTAAATAATTCAAATTCTAAGTTATTTCAATCGTTGTTTAACACTCAATATAATCTAGGAATATTCAATGAAACAAAAGATTCATTTATTACATTTATTTGAGATTTTATTCAATTAACCATTATATTTACTGGCGCTTGATTATTTATAAAAAATAAAGATAATTTTACAAATATTACATTATATGGATCAATATCAATTTTTTTGAATTCCCCTATTGTAAATTTAGTATTCCTGATTTCAAATTCTTCAATAAATAAACAAGGAATAATAAGAATAAAATATTTATTAGAAATCACACAAAACAAGGATGGAAATATATCATTCAAACCCCAGAAGCAAAATAAAATAAACATATTAAATGCTTCAATAAATATTGGTTCAAAAAACGTTATAAACAATCTTAATTTAACAATTAAAAACAATCTAGAAATATCAGGGAAAAATGGAGTTGGTAAAAGTTTAATTTTGAATGCTATATTTAAAGTTTATTGATTAAATCAAGGCCACATTTTTGTCAATAATATAAATTTAAAGCATATTAGTCAAAAGTCATGATTAGATGCCTGCTTTTTAAATAAAAATAATGAATATTTTTGCTATGAAAATCTAATGGATTGCATATGTAAAAAAGACAATATAAAGGAAAATATTTTTCTTAAGAATTTCAAAAAATATAATTTAAATAACTTGTTTTTAGATATGAACTTACCTCTTAATAAATATATTGAAAATGGGGGAAATAATCTATCATCCGGCCAGAAGGAATTCATAATTATAATGCGACTTTTTTGCCAGAAATATTATTTTATTCTTTTGGATGAGGCTTTTGAAAACATTACAAATGAAATTTTTCTTAAATTAAAATTTTTAATTTTAAACTACCAAGATAGGGCTATTTTTATTGAAATTAGCCATAACAAAAAAATTGTTGCAAACAAGCGAAAAATTTTTGCTTTAAAAAAATAAATATTTTTTTGTTTTTATTTAGTTTAAAAAAGAATATTATCTTTATAATTAATAAAATATGAATAGAATTAATTTTAAAAACCAAAGCTTTCATCGTTTTAATTTTATGAAAGATTTCTTAGATATTTTAGAAGAATCAAAAATAGAATTTGATGCAAAAAAATCACTTTGTGTTGATGTAATGTTTGTAACTGCAAAAAAGATGAAAAAGTTAAATAACTTACATAGACAAAAAAACTATACAACTGATATTTTAAGTTTCCCACTAGATAGTCAAGAAGAATTAAATTTTTTGGATGAAATTGAATTAGGACAAATTATTATTTCTCCGTGGAAAATAAAAAAACAAGCCAAGGAGTTCAATCATAGTTTAAGACGAGAATTTTGTTATATTTTTGCTCATGGAATTGCTCACCTATTTGGTTTTGATCACATTACTGAAGAAGAAGCAAAAATAATGAATGGGCATGTCGATAATATAATGAATAAATTAAACATAAAGAGAATATAAAAATTGGAGAAAAATGAAAAAAGTTTGCAATGTATGCTTAATTGGGCGCCCAAATGTTGGAAAAAGCACGTTTTTAAATAATGTGTTAAATTTTAACCTATCAATAATTACGGATAAACCCCAAACAACAAAAGATAACATTAGAGGAATATACAATGATAAAGACTATCAAATCATTTTTATTGATACTCCAGGAATTCATAAAGCAGAAAATTTATTATCTGAAAGATTAAATAGTAAATCATATGAAGCTATTGAAAATTCTGATGTTGTTTTGTTTTTAACTCCCGCTAATGAAACAATCGGAACAGGAGATAAATTCATTATTGAAAAAATAAACGAAACTAATAATGAAAATAAAATTGCAGTTATTTCAAAAATTGATTTATTAAATACAAAAGAAGAAGCAGACAAAAAAGCAGAAGAATTAAAAAAACTTGGATTTGAAAAGGTTTTTGGCATTGGACAAAATTTGCCTACTTCTTATTCAGATTTGATCAATGAAATTAAAACCTATTGCTATGAAGATGAACCTTTGTACCCTGATGATCAAATTGGGGATATAACATTAAGATTTATGGCAAAAGAAATAATTAGAGAAACTGCTATAAACAATTTAAAATTTGAATTGCCACACTCAATTGCGGTTGAGATCGAACAATTTGACGACAAAAATGAAGATGAAGATTACAAGATCTATGCTTTAATTTATGTTAAAAAGAAGTCTCAGAAACCTATTTTAATCGGAAAAGACGGCCAAATGATTAAAAAAATCTCTATTGATTCACGACATAAAATGGAAAAAGTTTTTGATCACAAAATCTTTTTAAATATTAAAGTAAAAATCAGCGAAGATTGAGTAGATAATGAATTCAAAATAAAACAGTTAGGATATTAAGGTGAAACGATTAACTACACATAGAACGAGAGTTATTTTTATAGCAATAATTGCCTTACTAACACTTGGATTATTTTTAATAATTTTATTCTTTAAAATTAGCTCCCCATTTAAAGTAAGTATATATAACTACGAATCATACTTAGATAAGAAAATAATTACCAAATTAAAACAAAATTATTCATATCACGCCTTTACAAATCTAGATGAGTTTACTAGAGCAATAAATAACGAAAAAGCCGTTGCTGGAGTTGGAAGTGATTATCAAATTGCCCAACTAATTCTTGAAAAAAAATTAAGAAAAATTAACTTTGCAAAATTGTTTAATGATTCAAAATTTAATGATTCAACCCAAATAAAGAAGCTTTATCCAGAAATTGTTAATAAACATTTTGATTTATTTGAAAAATTTATCATTAAAAAAATAAGACAAATAAATCCTAAAAACATCAAGCAAAATGATTGACAAAATATTAGTCACACCAAATTCTTACCTTTTATTTATTATGACGAACATAACAATATTTTGGGTTTTGAAATTGACCAAAACCAAGGAGTTGACCACTTTTACGAATTTTTAATCCCTTATTTTATAATGGATAAAATGGTTGTTTATAATACAGATAAAAGTCCAAATAAATTTACTGAAAGAAGCAATTTAAAATCTAATGAAAACTTTAACGATTTAGAAAATTTAAATTCTTGAAAAGATATCATTAAGACGCTTGTTTCTAAATACAAAAACCCTAGAGTATATTGAACAAATTGATTTCAAGATAATGCTATGATTGGTCAATTTTATGGTTATGAATCTCTTAACAAATTAAAATATTTTTCTAAGCAAAACGAATGGGCAGATATAAATCAAGAAAATTTTAAAGAGATTATTAATGATTGGGTTTCTTTGGTTAAGGATGCAACAGGAGAAAGTATAAAAAATTCTAAAACAAATAAATTAGCCACTGATGGACAAGAATTAGTTAGTTCAATAATAGAGCCAATTAATGGCAAGGCAGATGTTTCAATTATGTATAATGGAGATGCCTTAGATGCATATTATGGGGAAGACAATTTTAAAAAATTAGGTAATATTCCGCATATTGGTTTTAAAAGACCAAAAAATACTTACATGAATATTGATGCTTGAATAATTTCAAGAGAAACATCTAAAAATGATGCCGATAATTTATTAAAAATATTATACGAAAATGTTATAAAGGGAGCTGAATATTCACTCCAAGATATTGAAATAAAATATTTGAAAGAAGTGTTGAATTTAATATCAAAAGACAACAAATTAAAAAAAGATGAAATTATTAATGAGCTATTTACAGACAAAAACATGTCTATGCCCAAAAAAATTAAAGACATTAAAAAAGATTTTTTTGCAGAATATTTTGACTATTTCAGAGAAGCATTTGAAGCAATGAATTTACCCATTGTAAACAATTTTCATTCAATAAATTACACACCAGGCTTTAAAAATGTAAAAAATTTTATTGAAAAATGGTACTTCTTGAATAAAAATCAGGAAGTTGATAAAACTGCTTTAGAGATATTTAATCCCGAATTTAATAAAAAAATCAATCACAGAATTTACCAACCACTTGATTTAAATTTAAAAACAAAAATAATAGATTATTACTTTCAAAAAACAAAATCATAATATTACTTAATTTCATGTTAGGAGAAAAATGGAAAATAAAATTAAAACACCAAACAAAAATGAAGAACCAATAATCCAATTAGTAGACGTTGTTAAAGAATTTGAAGATAAAACAGTTTTAGATAATGTTAACTTGTCAATAAATAAAGGTGAATTTATAACATTATTAGGTCCTTCGGGTTCAGGAAAAACAACTATATTGAGAATTATTGGTGGTTTTGAATGAGTTACTAGAGGTGAGGTTAAATTTTTTGGCAAAGATATCAAAGACTTAAGTCCACATAAAAGGGATGTTTCCACAATTTTTCAAGATTATGCATTATTCCCTCATTTAAATGTAGAAAACAACATTAAATACGGTCTTAGATTAAAAAGAATACCAAAAGATGATATCCCTAAAAGCTTTTACAATAAATTAAAATATCAACAGAAAAAATGAACAAAAAAAGCTTCTATCGAAATGAAAAAGCTTGATAAAACAATGGCGCAATATGAAAAAATTCTTGAAAACCAAAAACTTCCCGAAAAGAAACGGAACAAATACCAATCTTGAATTGATGACATGGATTTCAAATACTCATATTGAGAAAATTTTAAGGATTTAAAAACTGAAAATTATGAAAAAAGATATCTAACAAGAAAAATAACAAATAACGAAATGAACCAAGAAGTATCGGAAATCATGGATCTTGTTGGTTTGAAGGGAAATGAAAAGAAAAATGTTAATTTCCTATCTGGTGGTATGAAACAACGTGTTGCACTTGCTCGCTCGCTTGTTATTGAACCTGAAATTCTTCTACTAGATGAACCATTAAGTGCTTTGGATGCTAAAATTAGGCAAAAAATGCAAACATTATTAAGGGATATTCAAAAAGAATTAAAAATTACGTTCATTTTTGTTACTCACGATAGAAATGAAGCATTGCAATTAAGTGATAGAATCGCCATTATTAGAGATGGTAAGGTTGAACAATTTGCTTCGCCAAAAGATTTATATGATTATCCAGTAAATAAATGAGTAGCAAATTTTATAGGCGAATCTAATTTCTTTGATGCTGTATTTATTGATGAAAATAAAGTTAAATTTATGGGCAAAATTTTTGATACTATTCACAAAGAATTTGAACCAGGAGAAAACCTTGATGCATTAATTAGACCCGAAGATGTTTATGTTACCAAAGTAAAAGCTAAATCCAAATTTATTGGCAAAATAATTAAATCAACATATAGTGGTAGTTACTACAATTATGAGGTTTTAGTAAAAAATAAAATAATAAAAATGGAAACATCAATAAACTATGAAGTGGGTAAGGAAGTATATTTAGATTGATACAAAGATTCAATTCATTTGATGAAAAAGGATATTAAATTTGCCAAGGAACTGGCAGAACAAAACGAGATTAACAATGGAAAAACAACTGAAATCAACTTCTTATAAAGAAATAGACAAAAAAACTAAAAAAAGTAGTAAATTTGCAAAATTCTTTTTGGGTGCAAACAAATTACAACTTTCTTTGCTAATTCCTTATCTAATATTTTCAATTTTATTTATTATTTTGCCATTAATTTTGATATTTATTTATGCATTCAAACCACTAGAAGGACAAGGTCATCTTGCAAACTGGGAAATAGCAGGTAAATCATCAACTTGAATGATTATTCTTAGATCAATCGGAACCGGAATATGTGCAGCATTTTTTGCATTAATTATTGGTTTTCCATATGCCTATATTATTGCAAGAAGCAAATCAATGATATTTAGAACTTTAGGATTAACATTGATTCTAAGCCCGCTTGCAATATTCACAATTTCAAAAGCTTTAGCAGTTAGAGGTTTATTTTCTGCTATATTTGACGAAAATGAATTAAATAATAATTTCTTTCTAATCTTTGGAATGGTGTATTTGTATATCCCTTTTATGGTAATGCCATTATATCAAGTTATTAAGGATATGCCTAACAATATAATTGAAGCATCTCAAGATTTGGGATATTCAAAAACGCAAACTGTTTTTAAAGTTATATTACCCTACTGTATGAAGGCTATATTGAGTGGTTTTGGAGTTGTTCTTATGATGTCAGCGACATCAATAATTCTTTCAGATAAATTGTTGCCAAATGGTAGTCAAAAGCAACTTATAGGAAATCTTATAAATCAGTTTGCTAATACAGCAAACCCTTACGACTTAGCAAATGCCTCTACATTAGTTATTATCACTTTGGCTGTTTTATTAACAATATATGGAATTATTTATGGAATACCTTATTTAATTTCGAAAAGAAAGCAAGGAGGAATATATGAATAAATTTAAAAGATTTTTGAAGTATTCTTATGTATTAATAATTCTTGCTTGTTTATATATTCCAATCATTTTTGGAACAATATATAGTTTTAATGCACCTTCGGATAAGGGAATATTTTCTGTAACCTCATGAAATAGAACTTCTTATGAAGCGTATGTGGAACTTTTTTCAAAATCACATTGACTTGCATTTGTTAATTCTTTTTTACTAGGAATTTCAACATCTATTTTTGTTATATTACTTTCGTTGATGACGGTTTTTGCACTTTGAAGGCATAAAAACAAAACAGTTAGAGCATATGTGCAATCAACATCAAATATTCCTTTAATTAATCCAGATGTTATTACCGGTTTAACACTAGCAATTGTTTTAAATCTTATATTTTTTACCACACTAAAAGCAACCAACGAAGGATTTTTTAGAGCAATAATTTCACATACAGTAATGACTCTTCCTTATGGAATATTAATTATGTTGCCAAAAAGTGATAAATTTTCAAAAAATATCTTTGAAGCTAGCCAAGATCTAGGTTATTCAAAATTTAAAACATGATTTAAAACTTATTTTGTTTATATGTTAGGTTCAATAGGGTTTACATTTGTAATCACAATGACGCTTTCTTTTGATGACTTCATAATTACTAGAATTGTTTCAAATACAGAAACCTTGGGTACTCAATTATATGAAGGTCAATTTCAAGCATGATCGTTAGCTATTGGTGGAATATCATTACTAGTAGTTTTAATGGCAAATACAATATATGTAGCCATTAAATCATCGCAATTTTCACATAAAAAAATATCATCAATCAATATTTCTTCAATAAAAAAATAATATAGGAGGTAATTTTATAAATTGAGATCACGAATTTTAAAAGCTATTAGTTTAATATTTTTACCCTTGATTTTTATTGCTTTATTCCTTGCTTTTTTGGTTATAAAATCAACTAATCAATATAGACCTAGCATCTATAACTATGAATCTTATTTAGCTCCAACCATTATTAACAAATTAAAATCAAAATACAATTTCAAAGAATTTAAAGAAGTTAGTGAGTTTACGCAAGCATTATTGCAAGATAAAGCTATTGCTGGAATTGGAAGCGACTTTCAAGCTGCTCAACTTATTTTAGATAGGAAGATTCAAAAAATAGATTTTAGTCTAGTTTATGGTGAAAAATACAAAGATTGAAAAGAAAGACAATTTCTATATACCCCAAATGTTAGAGAGCATATTTTAAGATTTGATGCTCTGATTTTAAATATTTTAGAATCTGATAAATCTAATAAATATAAAAACTTCGAAATAAAGAAAAATAAAGAAGGCAAACCTATAGCATTTAGAGACAAATTAAACAATAATAACAAAGAAGTTTGGGATCATTTTTATGATTATATCATTCCTTATTTTACCCAAGATAAAGGTATAGCTTACAATATAAATCCAAAGACTAGACCAAATTTAAATATAAACAACGCCATAGAAAAACTAGATAAAAACGACAAGGTTTTTTCTTGAAATGAAATCTTGCAAATTCTAAGTAAGAATAATTACACACATTTCGGTTGAACTCATGCTTATTTAGATAACATGATGATAGGGGCATTCAACTCCGGAGAAAAATGAGAAGAAGTTTTTACTAAAAAAATCGGTGACAACAAAATTTTTGATTTTAATGAATCAAACTATAAATTAGCTATTGATTCATTTATAAATTTTGTTAAAAATTCTACCGGAAATGATATAAAAAACAATAAGAAGCATTACTTTAGTGGCGATGGTCTTGAATTATTAAATCATCTAATTGAACCAAAAACTTCTAGATCGGATGCGTCAATAATGTACAATGGTGATGCATTGGATGCATTCTATTCTGAAGATAATTTTCAATCGGTTGAAAGTGGTTTAATAAGGTTTATTAGACCTAAAAACACATATATGCTAATGGATAATTGAATAATTTCAAAAAGCCTTTCAAAAAAAGAAACAACAACCTTTTTAAAAGCTATAAGAGACCATGTTATCAATATTGATAAGCACAAAAATAATATTCAAAATATTGAAAAAATATTTTTTAATCAATTTAAAAACAACATGACAAAACAATTAAAACAATCTGCTGAAGCTTCTTTTTTTGAAACCATACATAATATTGACAAAACTTTAAAAAATAAGTTAATTCAAGAATTTCTAAAAAATAAAGAATTTTTCAATATAAACGAAAAATCAACTAGCTTTGAAGAATGAAATAGCAAAAAGTTTAGAGAATTTTTAAAAAATCCTACTATTCAAGAAAATTATGAGTGGTTTTTAATTTGAAGAAACAATTATCAAGACCATAATGAAGCTTTTCTATTTGATGATCTCATGTCTGATGCTTTTGCAAATAGTGAAATAGGAAGCATATCTAATTTTGATTATGTATCATATACTCCAACTGAAAAAATAATTTATGACTTTATTAAAAAATGATATTTTGGTTCAAATAAAGTGGCAATATCAATATTCGATCAACCCGAATCAAATAACAATTACCAAGTTTTTTCATATCCAATTATTGACAATAATTTAAGAACAAAAATCATTACTTATTATTATGAAAAAACCAAGTCCTAAAATTAAAATATCTATTCATAAATGTTTAATAAGATTTACAAAATTTAAATAATATATAATTTTTAGTATAAATTAAAAATTGAAAGGAAGAAAACATCATGAACATAAAGCAATTATATAAGCAAATAAGCCAGTTGCAAGAAAATGTTGAACTTACAATTGAAGGCTGAATTATTTCAAACCGCGGAAACGATAAAATTCGTTTTATTACAATTAATGATGGTAGTACAATTGCCAACATGCAATTAGTTATTAAGGGAGATCTAGCATCAAAGAAAGAAATTTCTGAAATTGCCATGGGCTCGGCAGTTAGTGTAATAGGTAAATTACATTTAACGCCAGAAGCAATGCAACCATTTGAACTAAGTGTCTCAAAATTTTTATACATAAACAATCCACAAGAAGATTTTCCAATCCAAAAGAAAGAAACATCTCTTGATTTTTTAAGAGAAATTCCTCAATTAAGACATAGAACAAGTTTATTTAGAGCAATTATGTTAATCAGAAACTCTCTTATGTTTGAAATTCATAAATATTTCCAATCTAAAGGCTTTTTGAATCTTTCATCTCCAATAATCACATCAAACGATGGTGAAGGAGCAGGAGAAACTCTGCTTGTTGACGATGAAAGCAAAGATTATTTTTTCAAACAAAAAGCATTTTTAGGTGTAACTGGACAATTGCACGCCGAATCATATGCTGAAGGATTTAAAAAAGTTTATACATTCGGACCAACATTTAGAGCTGAAAATTCTCACACTTCGCGTCATCTAGCAGAATTTTGAATGATGGAACCAGAAGTAGCCTTTTGTGATTTAAAAGGTATCATAGAAATTACAGACGATATGTTAAAGAATGTTATTGCAAATACATTAAAAAACTATCCCGAAGAAATGAAATATTTGGATTCATTACAAAACAATAAATTAATTGAATCATTAAACAACTTTATCAATAAGAAATTAACAATAATTGAATATAAAGACGTTGTTGAAAATCTTAAGAACTATGTAGATAAATTCGAAGAAAAAGATATCTTTTTTGGAATGGACTTAGCTTCAGAACACGAAAAATTCCTTGCCGAAAACATCGTAAAAGGCCCTGTTGCAGTTATTAATTATCCAAAAGACATAAAGGCTTTTTATATGTATCAAAATGATGATGGCAAAACAGTCGCTGCCTTTGACTTACTAGTACCAGGAATTGGCGAATTAGTAGGGGGAAGTCAACGTGAAAATAGATATCAAAAACTTGTTGATAGAATCAATGAACTAAAAATCACTCAAGAAACATTACAATGATACTTGGACTTAAGAAGATTTGGTTACGCCCCAAGTAGTGGTTTTGGTTTAGGCTTTGAAAGACTTGTAATGTATGTTACTGGCACAAGTAACATAAGAGATGTTATTCCTTTTCCTCGTGTTGCCGGCTCAATTAAAATGTAGGTTTCTATGCAAGAAAATAATTTAACAATATTAATACCTATATATCATCCAACTCTAACAATTAATGAGATATTAAAAAATTTATATAAACAAAAACAACAAAATTTTAATTTAGTAATCGCTATAGATAAGCCTTCTGAATCAGAACTTTATGAATTAGAAAAAGCAAAAACCTTTTTTTCAAATAGACTTCAAATAATAATCAATACAAATCACCAACACATTGATTCAATAATTAGTAGTTGCTTAAACATTACAAAAACAAAATACACATTTATTATGTATTCATATATTGAAATTGAGTATGATTTTATTTCGAAAATAAATAAACTTTTATTAGAATTTAAAGATGTTGATATTATAAGTTTTGACGCATATACAAAGGGTATATCTTGAATGAATTTCAACAAATATCCTAAACAAAATAAAGAAATAAATATTCATATTGAAGAAGAAAATGTTGCCAAAATAATTCCTTTTATGTACAATTTTATTGCAAAAACTTCTTTACTTATTGAAGCAACAAATCAACACAATAATAAACACCTTAGCGAGCAATATTCACCCAATATACTTTTTAAGACTTTAATTAATGCAAACAAAATTCTTTTAACAAAAGATATTCAAGTAATTGATTGAAATTATGATGTTTTATTGTGAAGCGTTAAATCTTTATTAGAATCTTGAAAAGACATAGAATCTGAGTATTCTGCACATGAATTTACAACTCCTTTTTCAGAAAGCTATTATTATCTTGCGAAATTTTTAAATATAGCATATTGTTTCACAGGTTTTTTAGGCCAATGTCAATTTAAATCAAACACAAAAAACTATCTAACCTTAAAAAACCTTAAAAAACATTTAGAATCATCGATAAGTGAAAACATTGAAGAATGAAAAAATAGCAAAATTATCAATGATTTTTTAGAAAAAAACCGTATTCAACAACTTTTTGAATTAATACCAAATAGTAAAAAATGATCACTTATTTTTAAAAAAATAATATGATAAAAATAAATATTAAAGCTAATTTTTGAATAAGACTTGCTGCTACTTTGATAGATTTCTTGGTATTCATTGGTTTTTTAATCGGTTCTTCGTTTGCAATATTTAACTACAAGGAAGCAAAATTTTATAATTATTGATCTTATTATCTTTGAATAATAATGATAATTTTAGTCCAAAATATAATTTGAATAATCATTCCAATTTTTACTAAGGGCTATACTTTGGGTTTGTTTATTTGCAGACTAAAAATAATCAAACAGCAAAACAATAAATCGAAACTTAGCAAGGTTATTTTTGATAGGCAAAGGTTATTTAGTTTTATATGAATGATAATTTTTGCTTTATTTATGATTTTTATATCTCCTCAAACTTTTGAACAAGCAGCAAATCTTTCTCAAACAAAACCTTTAGACCAACTTCAAAAAGCTTTTCTTTCAATTCCTAGTGTGCTTTCAGCATTAGCCACATTTTTAGAATTCTTATTTATTTTAAGCAATGCTAAGGGAGATAAAATTGGTTTAAATGATAAATTTTCCGGATCCTATACAGTTTGAGCTAAAAAATATGAAGATATTGAAGAAAACAATATCAACGATTTGATTATAAAACCCGTTGTTAGAGAATTACCAAAAATTGATTACAAATAGCAAAAAACAAATATTTAGATTTTGGTTAATTTTAATTGAAATATGCACACCACAGAGGGGGTGTTGCTTTTTTATATTGCACTTTTGTGATATAATTTTATAATAAAATTTGCACATAAAAGGGGAATAAAATGATTGATTTAAAAGATTTAAATGAACAGCAAAAAAGTGCCGTTATATATAACGAAGGACCTTTAAGAATTATTGCTGGCGCTGGTTCAGGAAAAACTAGAGTTTTAACATATAAGATTGCTTATTTAATCCAAAAATTAGGTGTTCTACCTAATCGTATTTTGGCACTAACTTTTTCAAATAAGGCTGCAAACGAAATGAAACAAAGGGTATTTAATCTACTTGCAGAAGATAACCCTAGCAATCTTTCTCCCTATATTTCAACATTCCATGCAATGTGTGCAAAAATATTGAGAAAAGAAATATATAACTTTGGATATGAAAACGATTTTCAAATTCTTGATGAATTAGACCAAAAAGAAATATTAAAAATTGTTTACTCAGAACTTGATATTTCACCAACAGAATTTACATTCTCTTCAATAATTTCATTTATTCAAAACAAAAAGAATTCAATCGAATATTTAAACAACTCAATTAGCGATAGTAGCGAACACTTTAATGAAGACTCAGATACACAAGAAACACAAGATGAAAAAATAAAGACAATAAAAGAAAAAATTTACGAACGCTACCAATTACACCTTGAAAGATCTCACTCCCTTGATTTTGATGATTTGTTGGTATTTGTATACAAATTATTTTATGAACCAAAATTCAGTTTTGTAGCTGAAAGATGAAAAAAGAGATTTGATTATATTTTAATAGATGAATTTCAAGATACCAGCATTTTGCAATATAGAATAATGCAAAAACTTGCAAATGAACACTTAACCATTGTTGGTGATCCAGATCAAACAATTTATTCTTGAAGAAATGCTGACATAAACATTATTATGAATTTTGATAAGGATTACCCAAATTCAGTTACAGTAAAACTTGAAGAAAATTACAGATCAACCAAAACTATTTTAAGAGCAGCAAACAACTTAATTTCTCATAATAAACTTAGATTAGAAAAGAAATTATTCACCGAAAACGAAGAAGGCGAAGATATTGAGTTTTATTGTGGTTTTAATGACGAAGCAGAAGCCAGATGAATAGCAACAAAAATTTCTGAATTAAAAAGAAATCGAGTTCAATTAAAAAATATTGCTATTTTATATAGAACCAATAGTTGTTCAAGAGCAATCGAAGAAGCCTTAATCAAAGAAAACACAATATATAAATTATTTGGCTCTATTAAATTTTACCAAAGAGAAGAAGTAAAAGATGCTCTAGCATATTTAAGAGTTATTCATGACGGAAGCGAAATTTCGTTATTAAGAATAATTAATAAGCCTTCTCGTAAAATAGGGAATGTTACAATCGAAAAACTTCTTTCTGCTTCTAAAAAATACAACTTAGGACTTTTTGAATATCTAGAAACTCACTTTAACCAAATTCAAAAAGAATTACATATTTCTGGCGATACACTAAAAAACTTAGCAAACTTTATCAATGAAATTAGATGAGCTAGAAGAGCAATCCAAACAAATGCAATTCACTTAACCATAAAAGAATTAATCGTTAATAAAATAAAATACTTTGATGAAATAAAAAACTCAGAAGAAGAATATGAAAATAAAATCGACAACTTCAATAGTTTAATTGAAGCCATTGCTGAATGAGAAAAGAAAAACCCACAAGGTTCTATTGATGAATATTTACAAGAAATAACTCTAATAACAGATAGAGATGTCGAAGATGATGCCGCAAGTTTTGTTTCACTAATGACTGTTCACAACGCAAAAGGACTTGAATTTGATTATGTATTTGTTGCTTGCTTAGCAGAAAATATCTTTCCTCTAAAGAGGGCCATATCGATTTCTCCACAAGACGATTTTACTTTTTTAAATTCAAAAAATTTCAAAGAAAACATAGAAGGATTAGAGGAAGAAAGGCGTTTGGCTTATGTAGCAATGACTCGTGCTAAAAAAAGATTAATGCTTTCTTTCTCGGTTGGTAGAAATGGTATAAATAGAAAAAGTAGATTCTTGTCTGAAGCAGGAATAAACGAAATTAGAACAATAAAGATTGCCAACAACTTTTCAATTGCAGCAGAGCAAGAAACAAGAAATAACGATTTAATAGTCGGTGATTATATTATTCACAACACATATGGAAAAGGGTTGATTATAGATATGGTTGACAACGTTATTGAAGTTAAATTTGAAAATGATAAAAAGATTCGCAAATTATCAAAAACCCACCATTCAATTAAAAAATACGAAGATGACGAAAGTCTAAATAGTTAAAATGTATAAATACATAGCAATATGAATCTTTCTAATAATTTTATTCTTGGCTGTATCAACCGTATTAACGATATATTACGTTAATATTAGACAAAAGAAGATAAATGCACAAACAGGATTTTTTGTATTTAAAATAGACAGTACAAAAAACAGAATAAAAATCGAAGGCGATATAAATTCAATTAACAAGACACCTAAATATTTATGAAAAATTTTAAGCATAGATGGACAATGAGCAAAACTAAATAACTTCCTAAATATTTTTGATTCAGGTGATAAGCGTCTATTACTGGACAATATAAAGAAAAAAAACTTCTTTAAACTAAAAACAAAAATAGTAGAAGAATCAAGATCTAACTATTTTTCAAAAGTAGATATTGAGTTTAACAATGTTGTAAATGATGAAATTATTGGGACATTTACATGAAGAGACATTGACATTAAAATGGATAATGTTTTCAACAAAGAAATTCATGATTTATCATATATTCCAACCATTGAAAATTATGAAGGTTTGTTTTTCTTTTTAAACAAGAAACACATTACTGATATAAATACTTTTTTAGACTTGTTTAAAGATTTAGGAAAGCAACACAATATTAACGGCTTAGAAGTTGTATATGAATGAAATGTTCTTTGCATCTTATTTAATCATGATAAATTAGGCATTGAAAAAGCAAAATTTCTTGCACAAAGGTTTATTGATATTGCAAAGCTATACAACAAACTTTATTTTAAACTTTGTTGGTTTGATTCATATATAATGGATAAAAAAACATTAGATAACGTATTTACATTCTTTGATTATTTAAAATTAGAATCATTTGAAAAAAATGCATATAACTTTCAAAAAATTCCAAGCAATATTTGAACTCAACAAGACTTTCAAAAGTTCTGTTTGACATATGAAAACATTATTTATAACCTAAGAAATAATTTTTCTATTTCACTAAAAAATATTTTTTTAAAAAATGCAATTGGCGATAAAACCGAACTTAGAAGGTTTGGACTTCCTAAGATTTTTAATTCATTAAAATTAAACAAAGATTATGACTATTACCTAGAATTTTTAGACATAACTAGATTGTTTTATAATGTCTTTTACGAAAAAATCGGTGAACTACATCCTAAAAATGCAATATTAAAAATTAGTGATTCAATATTTGAAACATTAGATATAAACGTTATTAAAAAAATCGGGGTTGAATTTCCAACATTTATTCAATCAATAAAATTATTATCAGCAGAAAGAATAAAAGACATTTCCCAAAAAATTCTTAAACTTCAAGGAACAAAACTCAATTTTTGTCTAGAAATTAAGGATATTGATTCAACAATAATTTCTCTGGTATCCCCTTGAGTTAAATTCATTTGAATAGATGAAGAACTAACCTCTAGATTGACCGAGCCTAACATTGTTTTATATCTAAATACCTTATTAGATAACGTAAAAAATCTTGGTATTAAAGTTATTTTTGAAAAATTAGACTACAAAACATACAGGAAATTATTTTATAAACACACATCGATTATTTTATATACGACCAATTAGCGTTTTAATATAAAATTATTAAAAAATCAAAACAAAAAAGGACAAAACATGAACAAAAAACTTATTCCAATAGATTTCTTTGATGCAATAAATAAAAAGTGGGTAGATAGCCACAAGATCCCTGATTCTAAAAGCTCTATAGGTTGCTTTGCACAATTAGATAAAAATATTACCACATTAACTAAAAAAATATTAAATACATGAAGTGAAGATGCTTCAACAATTCCAAACGACCCTATTATTAATCAATTAGTAAAATTTTATAACTTAACCAAAAATTGAAAATTACGTTATCAAGCGCAATTAAAGCCAATTAATAAATTACTTTCAAAAATTAATTCCTTAAATTCTTGAAAGGATGTTGAAAACAATTTTTTGGAATTGAAATTATCTAGTTTAAACACCCCTTTAATTTTTTCAGTTTCAACAGATTTTAAAAACAGCGATGTTCAAACTCTTTATTTAGATGTAAACTCTACAATTCTACCAGACAAAAGTTTTTATACTGATCCTGTTAAAAAAGAAAAATTTATTAATGTTTGAAAAAATATGGTTTTAAAACTTTTAAATAAGATTGACAAAAACGAATCTAAAAATAATTTAATTATTGAAAATGCTTTAAAATGAGATGAATTAACTTCTTCATTTTTAAGAGATTCAGAATTCTATTTTGATATGGTTAAAATTTGCAATACTATTTCAACAAAAGAATTAGCCCAAAACGTTAAAACAATAAACATAGAAAATATATTAAGACAATTAATTAAATGTTTCCCCGAAGAAATAAATGCTATTGATCCAAATTCTATAAACATTGCAAAATCTTTAACTAATGAAGAAAATTTTGAATATTATAAAGCAAGTTTAATTATTGATGCAATATTAACCTATGCACCTTATTTCGATTATTCAACAATTCAAATTGCTTCTGAATTTAATTTAGCAATAAACGGAAACAAAAAAATTGAATCAAAAGAAAAATATCAAATTAAACAAGCAAAACATTTTTTTGATATTCCTTTTGGAACTTACTATGGTAAAACCTATTTTGGATTAAAAAACAAGCAAACGGTCGAACACATGATAAAAAGCATGATAGGCGTTTATAAAGAACAGCTAGCAAAAAACTCTTGGTTAAGTAAACAAACAAAAGAAAAAGCAATAACAAAGCTTAACTCAATCGGTGTTTATGTTGGTTTTCCTGAAATAATTCAAGATCACTATAAATATTTAATAGTTGAAAAATTTGATGGATACAATGATTTATTTGAAAACGTTTTAAAGTTTAACAAAATTTTAGCTGAACACATTTTATCTGAATACGGAAAAACAGAAGACAAAAACCTTTGACATATGTCACCTGCTATGGTTAATGCTTATTATAATCCTTCAAAAAATGTAATAGTATTCCCTGCCGCAATCTTACAAAAACCTTTCTTTAGTGCAGATCAATCCTCTTCATCAAATTTTGGTGGAATTGGTGCTGTTATTGCTCATGAAATATCACACGGATTTGACAATAACGGAGCAAATTTCGATGAAAAAGGAAATATGATTAATTGATGAACAGAAGAAGATAAAAAGGCTTTTGAAGTTAAGACAAAAGAAATGATTAATTTGTTCGAAGGTAGAGAAACATCTGTTGGTAAATGTAACGGTACATTAACTGTTTCAGAAAACATAGCAGATGCTGGTGGTTTATCATGTGCTTTAGCAGCGGCAAAATTAGAAAAAGATTATAACCCTAAAGATTTTTATATAAATTTTGCAACAATTTGAAGAACAAAATACCGTCCAGAATTACAATCATTATTGTTAACTATTGATTGTCACGCTCCTGCTAAATTAAGAACAAATGTTCAAATACAAAACTCTGATGATTTTTATACAACTTTCAATGTCAAAAAAGGTGACCCAATGTATTTGCCACCTGAAAAAAGAGTAAAAATTTGATAATTCTAAACACAAAAAGCAACCGTGCGGTTGCTTTTTTAATTAACACAAATCATCTTTCTTTATTCCTAAAAGAACATATAACATCACTCGTTTAATTCTGCTATTTGTATATCTTTTCGAAGTACATAAATCAACAAATTCATTATAATTTTTTGCTATTTCAATATTCTTTTTAAAAAGATTTTCCATTCCTTCGGATATCAATGCTATATTTGCTAATTCTTCTTTAGAATAATTTTGTACCTTTTGTTTAAACTCATTATATTTATCTTCAATTTTTGAAAAATTTTTAGGAATGTTTATCGGTGTGAACATTGAAACATCTTCGCCTTCTTCTAACATTTTTCGTATCATTGTTGCCGAAGCATAATTATTATTTGCTATTAACGAATTGTGTGGAGCTGTTCTTTTTATGCAAAACGGTTTTACTGACAAATTATTAAAAACTATGTATTTGGTATATTCAAACCCCAAAATATCTTGTGGTATATTTTCAACACCTATTAATTTTTCTAGCGACAAGAAACAACTCAAAACATATGATTTTCCTGTTTTTAAATTCATTCTTACATCTTTGTTATATTGATCAAAATTTTTCTTTATTACGTTTGCTGCGTTTAAATATTTTTTTATATCATCAGTGTCAGAAACCCCAAAAACTAAATAATCAATACCTTCTTTTATTAACAAATCTATTGATTTTTTAGCAAAAATATGGGCTGCTTGTGTAGATGCAAAAAAGTCTAATTCAACAACTTTTGTTGCTCCATATTCAAGCGCAATGTTTTTTCTAACTTCAAAAGGTAAACATGCTATTTCGCCCCGTTGAACATAATCGCAAGATAATGCAACAATTATTTCAGGATCTTTGAAGATTTCTTTAATTTTATTAATTAAATAAATATGCCCATTGTGAAATGGGTTAAATTCAGCTATTATTCCTATTTTCATTTATTTTTTCTTTAATTCATCAAGGCTAATATCGCAAAAATCTTCAATAGAGCTATCTTCTCAATAATTTAATTCCACAGTTTCTTGGATCTCAATGTTTTTTTGATCGCCTTCACAATTTTTGTTTGAATCAAAATCTTCGAACATCACAATATCGCTTTGTTCTTTTTCTTTTTTTATTTGTTTTTTCTTGCTTGTTTTCTTTTTATTAAACATATTTAAATTATATCATTGCAAAAAATTACTTTTGTTTTTTTAATTGTGTGATTAAAATGTTAATAAATTTTTTCGAAATTTCTTTTTAATTTTAGTTGTTTTTTAAATTGTTAACATTATAAACATAGCTTATTATACTTATTAATTTAACAAAGCAAACCAATAACACCTAACATTAAACAAAAACATTTTTATATATTATTTAATATATAATTAAAATATGAAAAATAATATTCAAGATACAATAGTTGCTATTTCTTCAGGCAATGTAAATCAAGCAATATCTATTATCAGATTATCTGGTGAGGATTCAATTGAGATTGTTAAAAAAGTTTTTAGTGGTAAAGTCGGAAAAGATAAGACAATTACTTACGGAAATATCATCGATAATATAACAGGGAAAATTGTTGATGAGGTATTAGTTAATTGATTTATTGGTAATAAAAATTATACTGGAGAAAATACCGTTGAAATAAATGCTCATGGTGGAATTGTTGTAACAAACAAAATACTTAATCTATTAATTGCTAATGGTGCAAGGTTGGCAAACAGAGGAGAATTTACAAGACGAGCATTTTTAAACGGAAAAATTTCGTTAGATAAAGCCGAAGCGATAAATAGCTTAATTCATGCAAAAACAAATAAACAAGCAGAAATTGCTATTTCTCAATTTAATTCTAAGGACAATTTAATTATTGAAAATTTAATTAACGAACTACTTACTATAATTTCATTAATCGAAATTAATATTGATTATTCTGATTATAACGATATAAAAGAAATGGATGCTAACACCTTAACAAGTCAATTATCAAAATTTAATAGAAAATTAGATTCTATAATTAATAAATCAGAAAATGCTTGCGATATTTATAAAGGAATAAATGTTGCGATAGTAGGAAGACCCAACACTGGTAAATCATCACTTTTAAATTCTTTAATTGGTTCACAAAAAGCAATAGTAACAAATATACCCGGAACAACAAGAGACGTGGTAGAAGGCGAATTTCAGATTAATGGTTTTTTATTTAATTTGATTGATACAGCGGGTATAAGACAAACAAAAGATCTTGTTGAATCAATAGGTATTGAAAGATCTAAAAACGCAATAAAAGAAGCCAAAATTGTAATTCATATGCACGAACCATTTATTAAAGAAAATGAAGAAGATCAAATAATTAAAGAACTCTCAAAAGGTAAGGTATATATACCGGTAATGAACAAGCAAGATCTTTTAAACAAAGATCAATATCCTAAGAACATGGTTTTAATCTCAAGCAAAGAAAATAGTCTATGAGAATTAAAAAATGCGTTAGTAAAAAATTACTTGAATATTGATTTAGATGACCCTCAAATAATTTTTAATAATAGAAAACTTTCGTTATTGAAGCAAGCGAAGATTTCTATCAATACTGCATTAGAATCGCTAAGTCAAGGTTTGGGCCCTGAGGTGGTTATTTTAGATATAAACGAAGCTTGGGGGTCATTGAGTGAAATATTAAATAAAAAGCACGATAATGAAGCTCTACTAGATAATATATTTAGCAAATTTTGTTTAGGAAAATAATATGTATAAAATAAACGATAAAATAATTGTAAAAGCAACAGAACTAAGTTATGAAGGCTATGGGGTTGTGAGAGGTGATAAACAAACACTCTTTGTTGAAAATCTCTTACCTGATGAAGAAGCAGAAGTAGCCATTTATAAATCAAATTCAAAAATTGCTTATGGACGTACAGTTAATTTGATAAAAAAATCATGTAAAAGAGTTGAAGTTGTTGAGAAAGAATTGTTTGAAAGTGGTGCAGCGCCATTGATGGTTTTAAGTTACGAAGATCAATTAAAATTCAAACAAGAAATTGTTAATAATTTAATAAAAAGAAATATTGGATTTCAAAACATTGAACAAATTCAACCTAGTCCAAAAATATTAAATTACAGAAATAAAATAACCTTACATGTGAATTATAAAAACAATAATATTTATATTGGTTTCTATAAAAAAAACAGCAACAAACTAGTTATTCAAAATTCGCTTCCTCTTTGTCATGATGAACTTGATAAATATTACAAGGAACTTATTAGCGCCCTAAAACAACAAATTTTATCAAAAGATAATTGAATAAAAGAACTTAAACTTAGACAAATTACATTGAGGCATAGTAATTGAAGCCATAATATTGAAGTTATTTTTATTATTGAAAATCCAAATAAAAAAGGTTTTGATTTGTTGAAAAATAATTTGATTTTCTTATTGAAACCCAATGTAATTTCAATACAATGCCAATATATTAAAGAATCTAAAGATATATTCACTTCTTTGTATAAATCAATGCCCCAACAATTTAATATAAACAATAAAATATTCAATGTGAACAATGATTCTTTTTTTCAAGTAAATGAAGAACAAACTAGTTTAATATATAATCAAATTAAAAAATTCATAGATCAAGAAAATTCAACAAAAATAATTGATGTTTATTCAGGTGTTGGTAGCATTGGAATATCTGTTTGTGGCAATGACTCTCAACTTGAATTAATTGAAATAAATAAATCGGCGTGTGAGTGTGCAAAAATTAACATAAACCAAAATTTGATTAATCCAAAACTAGCGGATATAACAAATAGTTCAGCACAAGACTTTTTTAATAAAAAAGAAGCAGAATATTTTGAAAAATCGATAGTTATATTTGACCCACCAAGAACGGGTTTAACAAATAAAGAAATTGATATGCTAGAAAAATGCAATAGTGTAATTTATATGAGTTGCAATCCTCATACAATGATTAGGGATTTAAAATATTTTGAAGAAAAAGGGTATAGAATCGAAAGGCTAATACCTTATGATATGTTTCCTCAAACTTATCATATTGAAATGTTGTCGCTTTTAAAAAAATAAAAATTAGGTTTAATTAAATTACCTAATTTTTATTTATAACTTTTTAGCATATTTATACCATTTTCTTGTGCTTCAACGAATATTGAATAGCAATGTTCTTGTGAATGCGTCTAATAATGTTGCTCCCGTCATTAAAAACATGAAGGTGGCAGGACTGAAATAATGATGTCCTAATTGAATATACACAACAAGATATACAGCGCTCAATTGAACACCTAGCATAGTTATTAAAGAAACTAAATTTGGCATTCAAACATCATTGGCGCCCCATAAAGCCCTTAGGGTTGTAAGGTTTCCTATATCCAGAATAGCTCTTAGCATCATAATTCCCATAACACCATACCCAACAGAATTAATTATTTGAATATCTATTGAATAAAGTCTTAGCAAAGGCCAGGTTAATGAAACTCCTAATATTGAAAATAATGACTGTGCATAAAAGCCAAACTTTAATGCACTATTTCCGAGTTCATGAGCAGCTTCAAAATTTTGTTTTCCAATTTCGTTTGCAACAATTGCGCTTGATACATAACCCAAAGCAATCATTACTGCATTAAATATGTCATAGGAACTATTTACAGCTCTTCATAAACCAATGAATTGTTCATTCTTTCCTTCGAACCCCATCGGAATTGCTCTTGCAAGAAGATATTGTCCTATGAATCAAATTCCTGTTTCAAAAGCAATAGGAAGACCTAACTTAAGAATGTTTTTAACATATTTTGTGTTTCATGTTTCTTTAATTGAAATTTTATTGTTTTTGGTTATAAAGTGATAGGAAATTGAATAATCAATTGTTAAATGAAATATTGCCCCCAAAATTGGTTCAAGTGCAACCATTATAAAAAGATCTTCATTTGAAATCTTTCAAGGTAAAAACATAATTAAATAAGTTCAACTTGCACGTACAAATATACTGGTAATTAATCCAAATATTGAAAATTTATTTTTTTCAACAGATTGTAAACTGGCCGTAAAAATAACGGCTATTGAATATATGAATATGTCGCAAGTGGTTATTCTTAAGAATTTTATAGCCATTGTTGTTTCGGAAGTTACTGAAACCTTAAAAGGATTTGTATTAACTAGGGTAATTCCATTTATAGTTCCACCGTATAGCACCTTATTAAATTGATTCATTCTAGCTAAATCATTTATAGTGATGTTGTGATTTTTCAATAATTCAATGTTTGATTTAAAAAGATCTACACCTTCCTTGTTTTTTCAACCAAAAAGAGGAATGTTTTTTGTTCCAGAAGCTTGTAAAACTATTGGTGCAAGTCCAAACATAATAAAATAGACACAAGTATTTATTATTAATGATACATAAACGGCAGACCACAAAGCTTTTGGTATGTCTCTTATTTTGCTTTGTCCAAAAAGATTTGAGCATAAGACTAATGTTCCGCTTTGGTAAATAATAGGAATAAAGGTTATCATTTTGTAAACAGTTAATATTTTTGCAATCATATAAAAATAAAAACCATTGTATGAACCATCGGCGTGATAGACTTTTATATAATGCGAAGAAGCAAGAGAAGAAACTACCGATACTAAAGCAACAAAAAGTGTCTGAAAAAAGATTGGTAAAGTCAATTTGAAAATATACTTAAAGTGTTTAACTTTAAATAATTCACTTGATTTTTCAATAAAAATACTTAAACGATTTGTATTTCTTTGTTTACTGTTTTCTTTCTTTCTCATAACTCGTTAATTCTAACATATAAATTAAAATCCATTTAAACAACTTTATTATAATGTAAAATTTATATAAATAACGCTAAAATTTGCAAGGAGATTTACATGGGATTTTGAACAAACTTAAAAGATAAATTATTTGGTACCAAAGAAGAAAGAATAGCCAAAAAACAAGCAAAAATTGAAGCAAAAGAACAAAGACAATTAGAAAAGGAATTAATTAAAAAAAATAAGTTAAATACTTATATTGCAGGTCTTTCGAAATCAAATTCTTCATTTAATGAGCATATAAAAGAATTGCAAAACAAACATAATAAAATTGACGAAGAATTTTTTGAAGATTTGGAAGAAATGCTGATTATGTCTGATATTTCGATTAAGCTAGTGCAAATAATTATTAACGAATGTAAAAAAGAAGTTAGAAACGAAAATATAACTGATCCAAAATTAATAAACGAGATAATTGCTGACAAACTGTTTACAATTTATACATCAAATTCTGTTGTCGATACAACACTTAATATTAAAGATAATAGGTTAAATGTAATATTGGTTGTGGGCGTCAATGGTTCAGGAAAAACTACATCAATATCAAAAATTGCTAAGAAATTAATTGATGAAGGTAAAAAAGTATTGATTGCTGCAGGAGATACATTTAGAGCAGCAGCAGTTGAGCAATTAGAGATTTGAGCAAAAAGAGTTGGAGCTGATATTGTAACACCAAACGAAAACGAAGTGGATCCGGCGGCGGTTGTTTATAGGGCAATCGATATAGCAAAATCAAAAAAATACGATATATTGATTGTAGATACTGCGGGAAGGTTGCAAAATAAAGTAAATTTAATGAACGAACTTGCCAAAATCAATAGGGTTTTAGCCTCTAAAATTCCCGATGCACCTCATGAAAGTTTATTAGTTTTAGATGCAACAACCGGACAAAATGGTGTAATTCAGGCAAAGGTATTCAAGGAATCCACACCACTGACCGGAATCATTCTAACAAAAATGGACGGAACCTCAAAAGGTGGAATTGTTTTAACAATCAAAGATGAGATTGGTTTGTTTGTAAAATACATTGGTTTAGGTGAAAAAGTTGAAGATTTAGCGGAATTTGATTTAGATTCTTATATCTATGGTTTAACAAAAGGTATTAACGAATAATGGAAACAATTGAAGAACGAGATTACTATGTAAAGTTGTTTGAAAAATATGGTGATTTATTACCACAATCTCAAAAACAAGCTCTACAATTACTTTTATATGAAGATTTGTCATACGGGGAATTAGCCCAAGAATTAGCGATGTCTAGACCAGGGGCATACGACGCAGTTAAAAAAGCAAAATTGCACTTAAAACAAATTGATGAAAAGTTAAAACAAAGTTAAAAAATTTTCTATTTTTTGTTATTAAACAAATAATTTCTGAAAATAATTAAATTTAATATATAAAATATATATAAATTATTAACCAATAAAGAGGTAAGAATGTCTTATAAAAGAGTATTGATTAAACTTTCGGGTGAGGGCTTAGCAAATAAAGGTAAACACCTTGCAATTGATTATGAATTGGTTGAAAAATTTGCAAACCAATTAAAAACAATAATTAGTAGAAATATTGAAGTAGCGATTGTTGTCGGCGGCGGTAATTTTTGAAGAGGAACAAGTGCCGAAAAAAATGGTATGCAAAGGGTTAGAGCAGATTACATTGGTATGCTAGCTACAACAATGAATGCACTAGCTTTGCAATCAGGATTTGAGAGATTGAATTTGCATTGTAGAGTATTATCAAGCCTAACAATGGACCCAAAGGTTTGTGAAGTTTATATAAATGAAAAAGCTAAAAAATACCTAAGAGATGGAGAAGTTGTAATTTTTGCCGGGGGAACTGGACGCCCATTTTTTACAACTGATACAGCCTCAACATTATTTGCCAGTGAAATTGATGCGGATGCAATATTGATGGGAAAAAATAATGTTGATGGGGTATATGATAGTGATCCAAAGTTCAATAAAGACGCCAAAAAATTTCCAATCATTACATATGATGAATTGATAGAAAAGGAATTAAAAGTTATTGATCAAACCGCAGCATCAATGGCAAAAGAAAATAATATTGATATTTTAATATTTGATATTAATGAAGAAGATTCTTTAATAAGGGCAATAGAAAATAAAATACCTAACACAATAATAACTAGAGAAAGGGAAAAATAATGGAATTAGACAGTTATCTTTTAGAATTAGATGAAGAAATCAGCAAATCAATTACTAACTATGAAAATCAATTAATGAAAGTTGTTATAGGTAGAGCCAACCCTGTTTTAATCAGTAAAATTAAGTTAGAATATTATGGTTCACCAATTTCAGTAGAAGAAATAGCATCCATAACTACATCGGGGCCATTACAATTAATTGTTAAGCCTTATGATATGAGTGTATTAAAAAATATTGAAAAAGCAATCATGGACAACAAGATAAACGTTTCAATAATAAATGAAGGACACCAATTGAGATTGCAATATCCTCAAATGACCACTGAAAAAAGAGTAGAAATGACAAAGCAACTTGCTTCAATAACCGAACAAGCAAGAATAGGCGTAAGACAAGCAAGACAAGATATCAATAAAAAAATAAAAGCAGATAGCGACCTTTCAGAAGACATTCAAAAACATTATCTAGATAATATTCAAAAAGAAGTTGATAAATCAATAGAAAAAATTAATGAAATGTCAAAAACAAAAGAAAAAGAACTTTTGACAATATAAAATGCTTCGGCATTTTTATTTTTAATTAATCTTTTATTTATTTATAATTATTAAATAATGAAAAACTTAAAAATTAGAGCAAAATCTGCGATTATATTATTCAGTATATTGATTCCACTACTTATTTTAACTTATTTTGTTAAAAGTTTAGGAGTGGCAATTTATATGTTTTTTAGTGCATGGGCTTTGTATGAAGTTATTTCACACAACCAAAATAAAAGGCCTATTAATTTAGTTATATCCTTACTATCAATATTAATTTGAATAATACCAGTGAACTGATTTCTAAAAGATTGGGGAACATTTTGAGAAATGTATAGCAATGGACATTGGGTCTATGGCCTTGATAGAGATACATTGATTTCAATAATAAAAAATACAATATTCTTTAAATCAAATGCCTGGTCTGGTGCTCCGGGGTTGGGCTTAATATTGGTTTTCTTAATAACAACATTCTTACTCATTGTTGATGTAAAGAGTCAAAAAAATGCTAAGGATTGATTTAAGAATTATTTCATTACAATATTCGCTGTTATATTTATTCCATCTAGTTTCAAATTAATTTTTATGTATAACGTAATGAGTTTGTATTGACTATTCATATTATTCTTAATTCCTATTGTATGTGACAGTTTTGCTTATTTAATTGGCAGAGTTATAGGAAGAAAAATAATTAAGTTTGGCTTTGCACCTCACATAAGTCCTAAAAAAAGTTGAGAAGGGGCCATCGCTGGATATTTTATAGGCGCGTTATTTGTCTTTATATTTATGGGGATTAATTTTGGAAACTCTGATTCAATAAAAATATTAATAAATCAAAAGCAAATTATTACGGCAGTTTTTGTTCTTCCTGCAATTTCTATTATTGGTGACTTGGTATTTTCAGGAATAAAAAGAGCTCAAAATATTAAGGATTTTTCTGAATTAATAGCGGGTCATGGAGGAATTATGGATAGATTTGATTCGCTATCGTTTAATACGGTATTTTTAACTTTTATATTAATGATTTAAGTAAAATAAAATAAAAAAATCGTTATTACTTTTTAACGATTTTTTTATTAATATTAATATAATTAATAATAAAACTATTTAATGTAAATGAGGGGATAATTTATGCTTGATAAGACTTTTGTGCAATTATGCAAAGAAATAAAATTTGAATTTGATGAACAACAATTTTATGGTGCAAAAGTTTTGAGTGTTGAATCAAACTTAAATGAATGGAAATTTAAGATTGCCTTTGCAAACCATATTGACATTGAACAATACAAACAATTTAGAGAAAAATTAGTTAAAAGATTTGGCAAGGTTGAATTAATCTTGGCTATTGAGACAATTATAAGAAATAAACAAATTATTGTTGATTATTTAAATTATCTAATAGAATGTAACCCCAAGAAATATTGGCCTTTTGTGAAATATAATTATTCATGTATAACTACTTCAACAATTGAAATAGACGAAAAAACATTAACACTTTCCTTGCTAACAAATGAAATTTATGAAGTTTTTAAAAGTAATAAAGATCTTTTTAAAGAAGCGCTTGCTTCAATAGGATTTGATTTTTATAATGTAGTTTTTAATTTGGGATTAAACAATAAAAAAAGCGTTGCAAGCAAAAAACTAGACGGTTGAATTGAACAATTTAACAATTTAAAAGATAATGAAAATAACTTAAAAGAAAGCTTTTCAAAATCTAATTTTTATTCTAAAAAAGAATCAGCAAGACCAATTGAGATGGACTTAACTACGGCAAACGAAACATTTGAAGAAATAAAAATAACCACAAAAGGAGAAGTTTTTTTTATTGAAAGTAAAGTGCTAAAAAACAAAAAAATTCTATATACGCTAAAGATTTCAGATTATAAAGAAGCTATTCTTATTAAAAAATTTTATGATTCTGAAAACGAAATACCCGATATAAAGGTAGGGGACACGATTATTGTTACTGGAAAATTGAGTGATGATAATTTTACAAAATCAAAAGTTTTAATAGCAATGGGCAAGGAATGCTTCATAAAGACAGAACCATTACAAAAACTTATTGAAGATAACGAAGACAATAAAAGAGTTGAATTAGCCGCAAGATCTAATATGAGTGCTCAAGATGGAATTTCAACACCATCTGATTATATTGATGCTGCTAAATTTTATGGTCATAAGGCTATAGCATTGACCGACCTAGATGATGCGCAAGGCTTTCCTGAATTTTATAATTATGCCAAAAAGCAAAAGGATGTTAAGCCAATATACGGCGCAACATTAAGCACCGTTGATTCAGATAAAAAATTCTTCTACAATTTTAAAAATTTCAACTTAAAAGAAGAAGAATATGTTGTGTTTGACCTTGAAACAACAGGATTGAGCCCAAGATTGGATGACATTATTGAATTTGGTGCCACTATTATAAAAAATAATACTATTACTCAAAAAATTCAATTCTTTTTACAAACAAAACGTGAGCTATCAGCCTTTACTGTTGAAAGCAGTCACATTAATAATGAAATGTTAAAGAATGGTTATGAACAAGAAGAAGGCATTAAAAAAATTTATGAAATATTAAAGGATAAAATAGCAGTTGCTCACAATGCAAACTTCGATATAAATTTTTGCAAAGAAAAATTTGGCAAACTCGGCCTTGATAATTCTAAAATTTTTGGCCTTGATACATTGGCCTTATCTTATTTTTTAAACCCTGATGAATTTCGTCATTCTTTGGGTTATGTTGCTAAAAGATTCAATATTGTTTATGATACAACTGTTGCCCACCGTGGTGATTACGACGCTAATGTTTTGGCAAAGATTTGAATAAAAATGATTGACAAACTTGCAAAAGATTACAATATCCTAAACTCACAGGATTTAAACAATGTCGATAGCCAAAAAATACTTTCTCATAAATTTACATACGAAACAAGAATATTGGTAAAAAATCAAGCCGGACTTAAAAAATTATTCAAAATTATTTCGCGTTCTTTAACTTCTCAATTTAAAAAAAATCCACAAATTTACTTTGATCAATTGGGCAATGATGAAGATTTGTTTTTTGGATCAGCAACAAATCAATCATATGTTTGAGACCAAGTATTCACTGGAACAAATGAAAATTTGCTAAATTCACTAAAATATTATGATTATATTGAATTACCCCCTATATCATCATTTCAATATATGATTGATAGTAACCACGTTACATTAGATGAATTAAAATTTTTGTATAAAGATTTAATTTCAAAAGCAAAAAGTTTAAACAAAATGTGCGTTGCTGTTTCTGATTGTAGATATATTTATAATTATCAAAGATTAATTCATGAAATTTATATTGAGGCTCCAACTTTGGGGGGTGGGCTTCATTGGCTTAAGAAGAGACCATCTCATCCAGAATTTAGATATTTAACAACAAGAGAGATGTTAAAAGAATTTGCTTTTTTGAATGATAGTTATTTAATTAATGAAATAGTAATTGAAAATCCAAACAAAATTGCTAATGAAATTTCATCTAATATTCAAGTTATTAAAGATAAATTGTATGTTCCAACTTTTGACAATTCGCCTCAAAAATTAAGAGAAGTTGTTTATAGTACTTTAAAAGAAAGATACGGAGATAATCCTGATCCCATCATAGTTAAAAGAATTGAAAAGGAATTAAACCCAATTATAAAGTATGGCTATTCTGTTATTTATTGAATAAGTCATAAATTAGTTAAAAGAAGTAATGAAGATGGTTATCTAGTAGGTAGTAGAGGAAGTGTTGGTAGTTCAATCGTTGCTAATTTAGCAGGTATTTCAGAAGTAAATCCCTTACCACCACATTATTTATGCAAAAATTGTAAATATTTTGAATGAAACAGCAATAAAGAAATTTATTCAGGTTGAGATTTGCCGGACAAGCATTGTCCAAATTGCAACTCAATATTATCAAAAGATGGGCACAATATTCCGTTTGAAACATTTTTAGGATTTGAAGCGGATAAGGTTCCTGATATCGATTTAAATTTTAGTGGTGAATATCAACCAACAATTCACAATTATGTTAAGGAATTGTTTGGTGAAGCACATACATATAGAGCCGGTACAATAGCTACCGTAGCAGAAAAAACAGCATATGGATTTTGTAAAAAATATGAAGAAGAAAAGGGGGGAAATTATCAACCTTGAAGTCATTTGTTCTTAGATTTTTTAGCATCTAAAACACAAGGGGTAAAGAGGACAACTGGACAACACCCGGGAGGAATTATTATTATTCCTAAAGAATTCGATGTAGAGGATTTTACGCCTGTAAACTATCCTGCAAACGACACAGAAAGCCCTTGAAAAACAACACACTTTGACTTCACTTCTATTCATGATAATGTCTTAAAATTAGATTTATTGGGTCATGATGATCCCACTGTTATTAGAATGCTTGAAACATTGACAAACACAAATGCAAAGGAAATACCAAAATTTGATGCAGAAGTTATGAAACTTTTCTATACAACTGAATCATTGAAAATTAGTCCTTCAGACATTGGAGGCGAAACAACTGGGGCTTACGGGTTACCTGAATTTGGAACAAATTTTGTAAGAAAAATGCTAAAAAAGGCTCAGCCAAGATCATTTAATGATTTAATTCTTCTTTCGGGGTTGAGTCATGGTACGGATGTTTGAACAGACAATGCTGAAGAGCTAGTGGCAAGAGGCAAAAAATTAATAGAATGTGTATGTTGTCGTGATGATATAATGCGTGGTTTGATTGAATGAAATATTGAGCCATTAACAGCTTTTCAAATTATGGAAAAAGTTAGAAAAGGAAAGGGCTTGACACCTGAGTGAGAAGAACTCTTAAAAAGTCATAATGTTCCTGAATGATATATAGAGTCGTTGCAAAAAATTAAATACATGTTCCCTAAGGCCCATGCTACTGCGTATGTAATTATGGCTTGAAGAATTGCTTGATATAAGTTATATTATCCATTGCCTTTTTATGCTTCATTTTATACAAACAGGCCAGATGCCATCGATATACAAATAATGAGTAGTGGCATAGCAAGCGTTCACCAAAAACTTCTTGAATTAAAAAATCGCCAAAATTCAAATGGCGAAAAATTATCTGTAAAGGAAGAAGCATTAATTCCTGTTTTAGAAATTACTCAAGAATTATATGCAAGGGGATTTAAAATTAAAAATGTTGATTTAAAACTTTCTTTGGCTGATAAATGAATAATAGATGAAAAAGATAAGGCGCTAATTCCTCCATTTAATGTTGTGGATGGTTTGGGAATTACTGTTGCTCAAACAATTGTATCTGCAAGAAAAGAAGGTTTTTTCCTTTCAATTGAAGACCTAGCTTCGAGAACAAAAGTCAATTCAAGAATTATTTCAGAATTAAAAAAAATAGGTGTTTTAGATTGCTTAGATGATACCAATCAAGTAAGTTTGTTTTAAAAATATCTTAAAATAAAAAGAAAGTGCAAATTTTAATAAATTAAAAGTGATATAATAAAAGAGCTCCAATTGGAGCATAAAGATCATTGTTTATTTAAATATATAGTTCGTTTATGTCAAATTAACTAAGATTTATTTGTTAATCTTTATATAAAAGTATAAGTTCAATAATTAAAAGTTTTATATGTTTTTCAACCTCCTTCATATTTTTTATTTATATACTTAAAACATAAAAAATGTGCGTATTTTAATTCAAAGAAATCTTATACAAAAAAATTTTTGTGTAGTTGCTTTAATTTGTTTTTGTTGTCTTATTGAATATATTTTAGGTTTTTGTAAATCCTTTCTTAAAAATGTTATTTCGTATTTGTGTGTTTGCAAAACCAAAGGGGTAAATTTATATTTAATAATATTTTTCTTCAAAAGCATCTATACAAAAATAAAGCTGTTTAAAAGTTTATATTTTTTATTAAAAAAATGTGTAGTTATTTCTTAAACAACGTCAGATAATAAGGCGTTGTTTTCATTTGCCTATTTTTTGTTTTTTAAAAAATGCTATATTTGGTAAAATTTTAATTAAATTAAATAATTATTTATATAAGAATGGAGCGTATTATGTCAAATAATGACAAGTGAAAAAATAAGAAAGTTAATTTAAAGAATTATGTTGTAGAATCAGGAAAGCCAAAAAGAGAATTGTCACGTTCATGAAAAATAGCTCTAACGGGTTTATTTTTAATTGTCATTCCTTCTTTTATAATGTTTTTAATTTTAGGCATAGATGGTTGAATTATAAAATCAACAAAAAATTTATCAAGATGAGGTGTTGAGTTTCCAATAGCATTGGCAATTGCTGCAATACAAATTATTATTGTTTTATTGTTAGTTTTTAAATTTAAGGTATTTAACACTGAAGCATTAACCTTTCTAATTCCAATTTCTTTAGCCATTAATTCATTTTTAGTATCTAGTGGTCAAAGACCAGAAGATTGATATATTAGGGTTTTACCCGCTGTAGGTTTGGTATTCCTTGCAATTCCAATAATATTAATAAACAAGGCAGTTGCTAAAAGCCAAGAAAAACAAAGAAAAATTAAATTACTTGAAGAAGAACAAAAAAATAAATCATTGCTTGACTAAACTAAACTTCGAGTTTAGTTTTTATATTTAAGGAGTTTAGATGTATAAAAAAATAAGTTTAGATGATAAAGAAATCGAAGAATTAATTAACCTAGAAAATCAACGTTTAGAAGACCATGTAGAACTAATAGCATCTGAAAATTATGTTTCAGAAGATGTTATGAAGGCAAATGGTTCTTGTTTAACTAATAAATATGCAGAAGGCTATCCTAATAAAAGATATTATGGTGGTTGCGAATATGTTGATAAAATAGAAGAAATTGCACAAGAAAGAGCAAAAAAATTATTCAATGTAAAATATGCTAATGTTCAACCATATTCAGGAAGTGTTGCAAATGCTGCTGTATACATGGCAATGGTTGATCCTGGAGATAAAGTCTTAGGATTAGATTTAAATTCAGGAGGACATTTATCACACGGATATAAAATTTCATTTAGTGGTAAATTTTACGAAGCACATACCTATTCTGTTAACGATCAAGGTTTATTGGATTACGACAAGATATTGGAAATTGCAAAAGAAGTAAAACCAAAAATGATAATTTGTGGATATTCTGCATATTCTCAAATTGTTGACTTTGCTAAATTTAGAAAAATTTGTGATGAAGTAGGCGCAAAATTATTTGCCGATATTTCTCATATATCAGGATTAATAATAGCAGGATTACACCCATCACCTTCGGGTTATGCTGATGTTATAATGACTACAACACATAAGACATTGCGTGGCACTCGTGGAGCAATAATTTTAACTAATGACGAAGAAATTTTTAAAAGAGTTAACAGTGCGGTTTTTCCTGGCGTTCAAGGAGGACCTTTGTTGCACCAAATAGCGGCAAAGGCAGTAAGTTTTAGAGAAGCATTACAACCAGAGTTTATTGATTATCAAAAGCAACTATTGTTGAACTCAAAAATTATGTGTCAAACATTTTTGAACAAGGGAGTAAAAATTATCTCTGGCCTTACTCAAAATCATTTATTTATGATTGATGTAAAAACTAGTTATGGAATAACAGGAAAATATGCAACAGAAGTTTTGGGTAGAGTTAATATAACCGTGAATAAAAACACTATTCCAAACGATAGTGAAAAACCAACCGTTGCTAGTGGAATAAGAATAGGATCTGCGGCAATGACATCAAGAGGATTAAAGGAAGATGAGTTCATCATTTTAGCCAATTTAATTGATAAAGTTTTAAGAGATCCAGAAAACGAAAACTTATTATATGTAGTAAAGAAAGAAATTGCAAAATTAACCAATTCATTTCCAATTAAACGTTCTCATTTAGTTCGTTAGAAGTAGGCATATGATATTAGCGGAAATAAAAAATTTAATTAAATCTTCAATAAGTATTATTCCAGGAATAGTTGATTTTGTTGAATTAACTTCAACCAATAATATAAATATTGAAAATGATGGTTTAATCATTGAGGAAGATCCACAAAATAAAAACATTATAAATATAACAATTGGTTTGAGAATAATGAGCAATTTAAGTGCTAAAAATATCGTTGAAGGAATATATGAAATCATTCCATATAACTTAACTTCTTACGATATAAAATTAGGAACATTAACAATATATATTAAAGGAACTAAATAATGAAAATTATTACAGGAAAAGAGTGAAATAAAGCCGTATATAATGGTTCGATAAACATAAATAACAAGAAAAACGCAATTAACGCTTTAAATGTTTTCCCTGTACCTGATGGTGATACAGGAAGCAATATGTCATCTACAATTTCTTCTGCATGTAATTCTGAATGCTTAGAAGACGATTGCAGTATTGAATCAGCTTCGAGAATTGTTGCTCAAAATATGTTAATGTCTGCCAGAGGAAATTCTGGTGTAATTTTAAGTCAAATTTTTAAAGGTTTTTCTAATGGGTTTGCATCTAAAAAGTCTGCTAATGCTTTTGATTTAGTTAATGCTTTCGAAATGGCTGCTAAATCAGCTTATGGTGCAGTTTTAAAACCTGTTGAAGGAACTATTTTAACCGTTATTAGAGAAGTTTCTGAAGGTTTAAAAAACCAAGTTGTGGTTGATACTACTATTGAAGAAGTTTTTGAAATTGCTGTTAAATTAGCAAGAAAAAGTTGTGATGGTACTCCTAAATTATTAAAAATATTAAAAGAAGTAGGTGTTACTGATTCAGGTGGTGAAGGTTTATATGCAATATTGCAGGGAATGAGTGCATACTTTAGTGGTAATAAAGTAGAACCTATTGTTGCAGAAGAGTCGGTTGAAAAATTTATTTCTGATGAAGAGGTATATGAAGGCGAATTTGGATATTGCACCGAATTTATTTTGGAATTATATAAACCAAAAAAATTCGACAAAGATTATTTAGTTCGTAAACTAGAAAAAATTGGTAATTCAATGGTTGTGGTAAATGATGACAAAATTTTAAAAGTTCATATTCATACCCCTAAGCCCGGTGATGTTTTAAATAGCGTAAATTCTTTGGGACAATTTGCAAAAATTAAAATAGATAACATGACTTTACAAGCAAACAATAGTAAGAAAGCTGCAAAGAAAAAACAAACCGAAGCGCCAAACAATGTTGAATTAAAAAATAGCGCCATTGTTTCGTGCAATACAGGCCAAGGAATCGTAACCTTAATGAAAGAAAACGGTGTTTCATATGTTGTTGATGGCGGTCAAACAAATAATCCTTCAATTCAAGATATTGTTAATGCAATAAATGCGGTCAATGCAAAAACGGTATTTGTATTGCCAAATAACTCAAATATAATTTTATCTGCTCAACAAGCCGGCACAATTATTAGAGATAAGAATGTAATTATCATTCCAACTAGGAGTCAAGTTCAAGGGTTAAATGTTGCATTGAATTATAGTTCTGAAAATAGTGATGAAGAAAATCACGAATTAATGAGCAATAGTTTAAAACATATAAGATATGGAGAAATTGCTGCCGCTATAAAAGATGCTAAATTAAACGGAATAAAGGTTAAAGCATCCGATTATATGGTTATTGTAGAAGGAAAATTACAAAAAACATCAAAGAGTCTATTCAACGCAGCACAATTATTAGTATCAGAATTAGTTTCGGATGATAGTCAAGTAGTAACAATAATATATGGCCAAGATGTTTCGGAAAATGATGCGCTAGAATTACAAAATTATATTGAAACAACATACGACCTTGTTGTAGAAATTTATCAAGGTGACCAAGGAATTTATCCATATTTAATCTCAGTCGAATAGGAAAAAACAATGAAAAAAATAGTATTTGATTTATTGAATAATGATAATGGCGAAGAACAAGCGATTTTGGGTGCAAAAAGATTTTTGCAAGAAAATTCAGATTATTTTTTAATTTTGGTTGGTCATGAACAACTTATCAAGAAACATTTATCAGATTACATTGATAGGATTGCCATAATTAATAATGAAACAATTGTACACATTGAATCTAGTCCAAGAGACGTATTGAGAACTCAAAATTCAATGCTAGAGGCTTTTAAACAACTCAACGAATCAAATGCAGATGGAATATTGAGTAGTGGAGATAGCGGCTCGTTAATAACATTATCATCATTGAAAGTTAAAAGGCTTCTTGGGATTGAAAGACCAGCATTCATGCCAATTCTACCAACAACAACGGATAAAAAAATGTTATTAATAGATGCGGGAGCAAACATTGAAATCAAACCAGAATATTTAAGCCAATGGGCTATGATAGCAAGTGAATGATATTCAATATTGTTCGGAAGCAAAAAGCCAATAGTAGGACAATTAAACGTAGGCGTCGAAGAATACAAAGGTTCTGAAATAACAAAGAATGCTAAAAATATATTAAAAGAAGTTTCTAAATCTGCTAATTTTGTTTTTAATGGTTATGTTGAAGCAACAAATGCCATTAATGGAGATATAGATATATTAGTGGTTGATGGATATGGCGGAAATGTGTTTCTAAAAACATTAGAAGGTACCTTTATTGCTTTTTCAAAATTATTGAAAAATATTTTATTAAAAAATTGAAAAACAAAATTAGCGGCATTAATTTTAAAAAGGCATCTAAGGGAATTTAAACAAAAATTTGATTATAGAAATACAGGCGGAGCGTTTATTATTGGGCTTGAAAAAATTGTTGTAAAGGCTCATGGGGGAAGCGACGAATTAGCATTTTATAACGCCCTTAACCAAATAAAAATAGGTATAGAAAACAATATTTCAACAATTTTAAATAAGAAATTCAAAGAGGCTGATAATGAAGAATAATTTTGAAGAAAATTTACAAAATGCATTGCTAAAATTTGGTATAAATTTTCAAGAATTAAATCAACAAAAGAGACATTATTTTTATAATGCTTTTACTCATAGTTCATACAGCAACGAACATAAAAATTTAGAAAGTTATCAATATTTAGAATTTTTAGGTGACTCAGTTTTAGGTTTTGTGGTTTCTGATTTCATTTTTGATAATTATAAAAATTTCGGTGAGGGAAAGGCAACAAAATTAAAAAGTGAAATTGTTGACAAAAATGCGCTTGGATTCTTATCGAGATATTATGGTTTTCCAAACTGTTTGAGGACAGGCAAAAGTGCATTTGCAAACGGAAAAGGAATTAAATCAGATTCAGATCTAATTGAATCATTCATTGGTGCTTGTTATAAAGCCTGCGGCTTGAAATTTACTAAAGAAATTATTTATCATTTTATTGACAAAATATTAAAAAAATTTCACAATTCAATCAATGAACACCCTAAAACAAAATTGCAAGAACTTGTACAAAGCAATTGAGGTTGTAGGCCTAGATATGAAGACGAAGCCTTAAATAACGGCAAATTTGAAAGTAAGGTCTTTATTAATAATAACTTATATGGTGAAGGTATAGGTCAAAGTAAAAAAGAATCCCAAACAAAAGCAGCAAGTGATGCTTTAAAAAAAATGGCAAAAATTTAATTTGGAAGGAAAGAAGTATTTATGAAGTTAATTCAAGTTGAAGCACACGGTTTTAAATCATTTGCAGATAAAGTAACCCTAAAATTTGACGGTGGAATTGTTGCAATAATAGGGCCTAATGGTTCAGGAAAATCAAACATAAATGACGCAATCAGATGGGTGTTGGGTGAAACTAGTTCCAAAGTATTGCGTGGAGATACAATGGAAGACGTTATTTTTTCAGGTTCTAAGACTGAAAAAGAAATGGATCGAGCAGAAGTAATTTTAACATTTGACAATAAGGATAGATCTTGTGATATCCCATATGATGTTTTTACTATTTCTAGAGTATTAACAAGGGGAAATGGTTCAAACGAATATTACATTAATGGTGAATTAGCAAGGCAAAGAGACATTAAAGAAATTGCAATGCAAAGTGGTATTTCAAAATCAAGTTTAGCAATCATAGGTCAAGGAACTATTTCCAACATTGCCGAATCAACTCCTGAAAAACGTAGAGAAATATTTGAAGATGCTGCTGGAACTTCAATGTACAAAAGCAAGAAGATTGAAGCACAAAGAAAATTAGAAAAAACCCAAGAAGCATTGGAACAAATTTCAATATTAATTCAAGAACAAGAAAGACAATTAAAGCCATTGCAACGTCAAGCTGAAAAAGCAAAAATTTATAAAACAAAAGCAGAGCAATTAAAAGAAGTCGAAGTTGCATTATTGGTTCATGATTTCATGGATTATTCAGATAAATTAGAAAAATTAAATGTTGAGGGGCAAGATTACAATCTAATTAAAGAAGAATTAGAAACCAAAATAAGAGTATACAACGAAGCAAGCGAACAAAAAAGCAAGTTTGTTTTAGAATTAGAAAACAACATTAAAAAGATAAGCGAAAGATTAAGCGAAATTAGCGAAGAAATTACGACCCTAGAAATAAGAAACGCAAAAGAAGCAAAACACCGCGAAATGCTTTTAAGTGGCGAACTAACTATATCCCCAAAAGAAAAATTAGCAGCAATGAAAGAAGAATTGAATGCATTAAATACTAATATTTTAGGGTATAAGACATTCTTAGAAACTAAAAAGAAGGAATCTGAACAATTATCTCAATCAGCTTCAATTAGATCAACAAAAATAAATGATATAAAACGAGAATTATCCTTTAAAAAAGAAGAACTTAATAAGATAAAATCAAAATTAGATGTTATTGAAGAAATAAAATCTAAAAAAACAAATTTGGCTAAAGGAACACATAATATTGTTGAAAACTCTCATTTATTTAGAGGATATAAAGCATTGGTTAGTGAATTAATTGAAGTTAATGAAAAATACGCCTTAGCTATTGAAACAATTCTAAGCTCCGCTATGCAACATATAGTTGTTGACAAACCAGAAACAGCAGTTGAAGCAATCAATTTCTTAAAATCAAATAATGGTGGTAGAGCAACTTTTATTCCCTTATCATCAATTAAACCTAAATTCGTTCCTGAACAACATATTGTTGTTGCTCAAACCCAAAAAGGTTTTCTAGGAATTGCTTCGGAACTTGTTTCAACAGAGGAACAATATAATGTTTTAAAGAAATTCTTACTAGGCAATGTCTTAGTTGCAGATAACATTGAAAATGCAAATAAATTATCCAAATTACTTGAAACTCGTTATATGGTTGTAACGCTAGATGGTGACATCATAAGAGTTGGTGGAGTTCTTTCAGGTGGACAAGCAAGTCAAAACGTTTCCACATTAGCAGCTGATGAACAAGTTAAAAAATTACAAGACCTTATTCCAGTAATTAATGCAAAAATTTCACAATTATCAAACGAGTTGTTTGAATTAGAAAAAGATCAAAATAAAGAAACATCATTATTGACAAATATTTCTCTTGAAAGAGCAAAGATTGACAACTTATATAGAACAACATTGGATAAGTTTGATTCATTACGTGAACAATATCGTAGTGCTTCGCAAGAAGAATATGAAGCAGAAAACAATAAAAAAATTGATATAGTAGCAGCAATTCAACAATTAATGCTTGAAAGAAGTGATTTATCGGCTAAAAAATCATCTCAAGAAGATATTTATCATAATCTAAAACAAGAATTACAAAATCTTACCAATCAACGCTTTCAAGTTAGTGAAGAATTAAATAATTTGATTAAAGATAACGCTAACAAAATAGCAGAAAAAGCAAAGGCAGAAACAATTATTCAAAATGCAAAGCAAAGATTGTCAGAACAATATGGAATGCTATTTGAAGTGGCAAAACAATTTTATAATCCAGATATAGATTTTGAAGTAGCAAGAAAAACAGTTGCTGATTTGAAATTGGATATAAGAGAGTTAGGACATGTAAACCTTGATTCAATTAATCAATTAGAAGAACTTGAAACAAGATATAAAGAAGTAAAATCTCAAGAACAAGAAATAATTTCAGCAAAGACCACCATTGAAGATGCAATATCAGAAATGGATAAAATTATTATTGAAAGAATGACGCAAACAGTTACATTAGTTAATGCTGAATTCAAGCATGTGTTTGCAAAAATGTTTGGTGGAGGTATGGCTGAAATAAGATATACTGACCCTGAAAACATTCTTGAAACAGGAATTGACGTCATTGCTCAACCTCCAGGAAAATCTATTAAGAATTTAAAATTATTTAGTGGGGGTGAAAAGGCACTTATAGCTATTTCATTACTATTTTCAATATTAAAAGCTAAGCCATTGCCATTGTGTATCTTAGACGAAGTTGAAGCAGCACTAGATGAAGCAAACGTAATAAGATTTGCTGAATATTTACACACTCTAAAAAAAGATACTCAATTTATAGTGATTACTCACCGTCAAGGAACAATGGAAAGAGTAAACAAACTTTATGGCGCAACAATGCAAAAAAGAGGGGTTACAACATTTTTTGCAGTAAATCTTTCAGATGCAAAGAAAACTATTGAAAACAACAATTAATTTTTTAAAAAACGTTAGCTTAAAAAAGAGCTAGCGTTTTTTAAAATTTTATTTAAATAAAAAATTTACCGAAAGGTTATATTTTTTGATTTTTTTAAAAATTTATTACAAAATAAGGTCTTTTTTTTTTTTTTTTTTAAGTTTCTAATTTTTAATATACAAAAATATACAACATTATATTTCTAATATTTAAATTTATCCTTAATTTAAATTAACAATTAAAAAAGAGGTAAGAATGAAAAAGTCTAAGAAAATATTATTAACTATGGGGGTAGTGCTTCCTTTATTAGCCTCAACTCCATTAGTTGCTGCTGGATGTGTAGAAACAAAGAAACCCGAAGTTAAACCAGAAGGTGAAAAACCAGGAACAACTCCTGAAAAGAAGCCTGAAGGTGAAAAACCAGGAACAACTCCTGAAGTTAAGCCAGAAGGTGAAACCACAATAGAAAACATCATTAAAAATCATAATGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTACTAAATGAATTAAATAAAGAAATTGAAAAAAGAGAAATTGAAAAAAGAGAAATTGAAAAAAGAGAAGAACAAAACCTTTTAGAAAAAAGAAACAACTTAATAAAAGAAACAGAAGCTAAATGAGATGATATTACTAAAAAAATAGATGAATATTCAAAAGATGCAACAATTTCTAAATTCCCAGAATTCACCCAAATAACTGATTATTTAAAATATAAAAACGTTGAATTAAAGAATTTAAAAGCGACAAAAAATAACACCATAAAATTATTGGTTGCCAAATATTTTAACATTTTAGAAGCCAAGATTCTTGTTGAAAACATCATTAAAAATCATAATGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTACTAAATGAATTAAATAAAGAAATTGAAAAAAGAGAAGAACAAAACCTTTTAGAAAAAAGAAACAACTTAATAAAAGAAACAGAAGCTAAATGAGATGATATTACTAAAAAAATAGATGAATATTCAAAAGATGCAACAATTTCTAAATTCCCAGAATTCACCCAAATAACTGATTATTTAAAATATAAAAACGTTGAATTAAAGAATTTAAAAGCGACAAAAAATAACACCATAAAATTATTGGTTGCCAAATATTTTAACATTTTAGAAGCCAAGATTCTTGTTGAAAACATCATTAAAAATCATAATGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTACTAAATGAATTAAATAAAGAAATTGAAAAAAGAGAAGAACAAAACCTTTTAGAAAAAAGAAACAACTTAATAAAAGAAACAGAAGCTAAATGAGATGATATTACTAAAAAAATAGAAGAATATTCAAAAGATGCAACAATTTCTAAATTCCCAGAATTCACCCAAATAACTGATTATTTAAAATATAAAAACGTTGAATTAAAGAATTTAAAAGCGACAAAAAATAACACCATAAAATTATTGGTTGCCAAATATTTTAACATTTTAGAAGCCAAGATTCTTGTTGAAAACATCATTAAAAATCATAATGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTACTAAATGAATTAAATAAAGAAATTGAAAAAAGAGAAATTGAAAAAAGAGAAGTTGAAAAAAGAGAAGAACAAAACCTTTTAGAAAAAAGAAACAACTTAATAAAAGAAACAGAAGCTAAATGAGATGATATTACTAAAAAAATAGATGAATATTCAAAAGATGCAACAATTTCTAAATTCCCAGAATTCACCCAAATAACTGATTATTTAAAATATAAAAACGTTGAATTAAAGAATTTAAAAGCGACAAAAAATAACACCATAAAATTATTGGTTGCCAAATATTTTAACATTTTAGAAGCCAAGATTCTTGTTGAAAACATCATTAAAAATCATAATGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTACTAAATGAATTAAATAAAGAAATTGAAAAAAATCAAGCATTAAGCGAAGCAAAGAAACAATTAGATTCAGCCCTAAAACACGCAAAAAAATTAAATATAGGGTCGCTTGATTTTAACAAAAAATTTAATTTTAATAAAGAATTTAATAATGAATATGCGAGGGCATTAGAAGCAATTAAAGATAACGATTTGAATGCTATTAAAGAAATTACATTCGAATTAAATAGTCTTATAAATTTAACGACATCACAAAAATCAAAAACAACATCAGAAATTAAAAACGATGTTGAAGGCGAAGTTCATGAATTAAATGATTTAATAGCAAAATTAAATGATTTTGTTTCTAATTATGTTGAAGAAACATTTAATGACATAGATGAAGCGGGTTTAATTAGTTCAATAGATAATTGAATCGACATGGTTGAAGGTTTTATTAAAGAATCTAAAAATCCTTCTAAATCGCTATATTTTAAATTAGAATATTTGAAATCTCAAACAAAAATATTTTATGAACAAAAAATGGAATCATGAGCAAACAAAGTATCTGATTTACTAATTGAAAAGGGATACGATCTTTTGGATAAAATTGCAAATATGAAAATGGTTAAAAACCTTGCCGGATATCAAAAAGAATTGGAAGATTGCTTAAATGAATTTGCTGAACTAGATGAAACCATTACCGTAGAAAAACAATTTGAGTTGAGCAAAAAACTTTATGATTTAATACCAAAAATTCAAAAAGAATTAGACAACGAACATAATAAGACCTTAAATAATGAATTAAATTAAAAATATTTGAAACAAAAAGGTAACATAAAACCTTTTTGTTTTTATTAGTTAATTTTTAGTAAGCTTAAATATAGAGGAATTTTATTAATATTTTTGTTATTAAAGCCTAAAAACGCAATATTTTCAATATTTTTTAGTTAAAAGTTAGATAATTTTTATAGTGTATTGTTAAACAAATACCATTTATGTCAATGACTAATGTTATATTACAATAAACAAGGAGAGCCTTATGTACGGGTTAGTTTTTTATTTAGATAAAGAAATATTAAAAAAAGAATACTCAGATAGTAATCATAAAGAAGCTTATGAAAAAGTAAGAGATATATTGAGGCACTACGGATTTCATTGATTATCAAATAGCTTTTATTTCTCGAGGTCAATTAATAATTTATTACAAGTCTATAAAGCAATAGAAGAATTAAAGACAATTTCGTGATTTATTAAATCACTAGTTAGCCTACATATTTTTAAAATGGAAGATTTATCAGATTTTACTGAATATTTAACAGGCGAAAGTGAAAAAATAGAAGAATAATTAAAAAAACTTGTAATGTATTAAATTGCAAGTTTTTTAAATTAATTGTAGTGAATCTTTTGGATTAAAATTAAATAAAAGATAGCAAAAAATCTCAAATGAACAATATTTTTTATAATTAGTTTATTGGAATATTTTAATATTTAAAAAGTAGCAATTTTGCTACTTTTTAAAATTGATAAATTATTAGTTTAATTTATATAGATGATTTGGGTTTTCCTTCAAAGCTTTTTCTAAAAACGATTGAATATCATTGTTTTCAATTTTAGAAATAAGAGTTTTAGCAGCTGATTCCCCTGCGCCGTAAAAGTCAAATGATTCAGGACTAAATGATTTCATTGTTGAAGGAATTTCTATTGTTTTTATTTGATTATCTGCCAAAGCTCCGCTTTCAACTAATTCAATGTTTTTTAAGTTTATGCTTGTAAGCTTATTTGAAGCAAAAACACCTTGTGTAAGAGTTTTTAAATAAGGTAAATCTTCTAAATTTACAGATGTAATGCCACTGTTTGCAAAGGCTTTTTGTCCTAACTTATTTAAAGATGCAGGCAATTGTAATTTTTTAATATTTGTATTTGAAAAAGCTTCAGCATCTATTTGAGATAAGTCGTTGCATTGAGAAAAATCTACTTCTTCTAGGTTACTTAATCCATAAAAAGCACCTGCGCCAATTGATTTTAAGGTTTTTGGCAAAATGATTTTTCTAATATTTATTTTTTTGTTGTTATTTGAAGAATTAGTTGTAACTAATGTTGATAATTCAGTTGGTAAGCCAGCACCATCGTCTGCCAAATTTTGCATAACTAGATAAGAAAATGATCCTGCGGGTATTGAAGTTATATTGGTTTTTGATAAATCTAGCAACCCTGATTCAGCAAATCAAGTTTGATTGTGATTGGATGAGTTCTTAGGGTCTTTTAGTGGGCCTTGCACATGATTTAGAGCAAAAGCATTGAACTTTTCTCCTCCACAAGATATTGCTGTTAATCCAGCAATAAATGGAGCAACTAAACCAAAGCCTAAAAATAAAAATTTTTTGTTCTTTTTCATAGTCAAAATTTTACCATAATTTTGTTATTTTAGTTTTTTATATTAAATGTCAAAAATAAATTACTTAATTTATATAATTAAATTAATAAAATCATGGAAAAGTTTAAATTAGTTTCAAATTATAAGCCAGCCGGCGACCAACCTCAGGCAATAAATGAGTTGGTTCACGGTGTTTTAGAAGGCAAAGAGCATCAAGTTTTATTAGGCGTAACTGGTTCAGGAAAAACATTTACTATGGCAAATGTCATTAATCAGTTAAATAGGCCTACATTGGTTATAAGCCATAACAAAACATTAGCAAGTCAACTTTATAGCGAATTAAGGGAATTATTTCCTGAAAATAGAGTTGAATATTTTGTTTCGTATTTTGATTTTTATAAGCCAGAAGCATACATTCCTAAGTCTGATTTATATATCGAAAAAACAAGTAGAAACAATAAAGATTTAGAAGCAATGAGAATGTCTGCTCTTAACGCCTTAAGTATTCGAAGAGATACTATTGTTGTTGCATCTGTTGCTGCAATATATGCCGAATTTAATCCTAAAGAATATATTCAAAGTTTTATGCCTATTGAAAGCCATATGAAAATTAAGTTGCGTGATCTTTTAACCAAGTTGGTTAATTTAGGTTATGAAAAACATAATAGTGATTTATCTTCTGGACAATTTTTCAATAAGGGGGAAGTTATTGAAATTTGTCCCGGATATACTTCAGAAAACAATATTCGTATTGAGATGTTTGATGATTATATTGAAAGAATTGCATTAATTAATCCTTTAACAAAGTCAATAGTTGAAGTGCGTAAGTCGTTGACTATTTTTCCTGCAACTACCTATAACGTAGAAAAAGGTTATATTGAAAATATTTGCAATAGCATTGAAAGCGAATTAAAAGAAAGAGTTGCCTATTTTAAATCACAAAATCAACTTTTGGAAGCCCAAAGGATTCACGATAAAACTTTAAATGATATTGATTCATTAAAAGAATTCGGTTATGTTAAAGGCATTGAAAATTATGCTCGATATGTTGATAAACGTGAACCCGGGCAAAAACCATACACAATATTTGATTATTTGCCAAAGGATGCAGTAATTTTTGTTGATGAAAGTCACCTTATGATTCCTCAAATAATAGGAATGTACGAAGGAGATCGTTCTAGGAAAGAAACACTAATTAAATATGGTTTTAGATTACCTTCTGCATTAGATAATAGACCCTTAAAATTTAATGAATATGAAGAATATAAATTTCCAAGAATTTATATGTCAGCAACCCCCGGTGACTATGAAATAAATAAGGCCAATGGTGAACTAGTTACTCAATACATTAGACCAACAGGCCTTTTAGATCCCATTATTGAAATACATTCAAAAGAAAATCAAATTCAAGACATTCATGATTTATTAAAATTACAAATAGAAAAAAAAGAAAGAACTTTAATTTTAACTACAACTAAAAAGAATGCCGAAGAACTTTCTTTATACCTTTCTGAATTTAAGATTAAAAGCGCATATATTCACGATGGATTTAAAACCTTTGAGAGAAACGAAATCTTAAAAGGTTTAAGAATGGGAAAATATGATGTTGTAATTGGAATTAATTTATTGCGTGAAGGTATTGATTTGCCAGAAGTTAGTTTAATTTGCGTATTGAATGCAGATTCTATGGGGCTAATGCGCGATGCTAGAAGTTTGATTCAAATAGTTGGCAGGGCATCTAGAAACGACCATGGAAAAGTAATATTTTATGCTGATAATATTACAACTAGTATGCAAAAAGCAATTGTTGACAATGAACATAAAAGGACGTTGCAAATTAAATATAATAAAGAACACAACATTACCCCTAAAAGTATTATTAAACCAATACCCGCACCTATTCAAGGCAGTATTTTATTAGAAGATAATAAAGCAATAAAAGAAGCCAATAGGACTATTCATAATAAAAAATATATTGATAATATGATTAAACATATGAAAGAACTAGCGAGGGCAAAAAAATACGAAGAAGCTATTGAAATAAGAGATTATTTGATTGAATTAGGTGTCGAATTTGATGATTAAAAAAAGGACTTTGTCCTAGTTTATAAATGGAATTACGAGTCTATTTTAAACCAATTTTTACTTTTTGTATTTTTCGTCATCAATAATTTTTTTAATTATTCCTTGCTGCAAATCAAAAATATTGTAGATTGTTGATAGCTCTTGAAATGTTTCTTTTAAAACACTTTTAGATGCTATTCATCCACAACCATCAGTAAATCAAACAAATGTAATATTTCCAGTCTCATCAAATAACCTTGACAAAATTCTATAACTTCTGGCAACCTCATTCAATTTTGAACCACTTGTATTGTAAAAATTACATTCGCATACAAAAATATGATTTTGATTATTTATAAAAATAAAGTCAAATTTTTTATTTATATTTAATAATGTGGGGTCAATATTAATATTCGTTTTCTTTTGAAAGCTATTTAAATCAATTTGTTTAAAATAGTTAACATTTTCAATTAAATTTGAATTTATTAAATAATGTTCAACAAGGTTTTCCATCATTGAGCCTGTTCTGTTTTTTCTTGTATTTGTATCAATTCCGACTTCAACACCAGTTGCATAGTCAACAAGGCTATTGTTTATATTATTCCTTATTAATTCAAACAAGCCTGTCTTTTCCATAAATAGTGTATATTCTTCTATACTATTTAGCTTTTTATCAAAATTAATTAGAATTTCTTTTTCAATTCCAACTCGAATATTATTACCTCTTATTGCTAATAAAGTTGGTATGGCTTTTAATACATTAGGATACATTTGTATTAAATTTTTAAAATCTGCTTTTATATTTTTTGATTTAATTAATGAATTTAAAATGCATAATTCAATTTTTATTTTTTCAATATTTTCATAAACTTTTTGGAAATTCACAAAGTAATCTCAATTACATATTCTGTTTTTTGAAAGTGTTTTTAAAAATTTTTCAAATCTTGTTTGCATAGAACCTCTTTATTAAAATTAGTAATGATTACCTCTTCGACATCGCCTCGATTTTTACCATTTCTATTAATGTTTCTTCTTGCTTTAATTATATTAATATTAAAATCCCTGTATAAATTGCAAACAAAATCAGTTTTGTGATTACTTAATATTACATAAACGCCCTTTTTTGACAACTTTTTAAAAACATTAGCAAGTTTAATTTGCTCGTTTTTTCCAAAGGTTTCATTTGAATAAGAAGTAAATGAATGTTTATTTTCTAAGGTATCATAAGGTGGATCAAAATAAACTAGGTCGCCAGCTTTTGCGTCTTTTATTGCATCTTCGAAATTTAAATTTGTTATTTTACAATCTACATTAGAAAAATATTCAAAAATAGAATTATAGTTTTCTTTATTATAGCAATTGACTTTTTCTTTCTTTCCTGATGGAACATTAAAAAAACCATTTTTGTTTACACGATATAAACCATTAAAACATGCTTTATTCAAATAAATAATTCTGGCAGCCTTTTCTTCATCTGACAAGTTTTTATATTCGGGTTGTCTATCTAAATTCCTTATTTGATAATAGTATTCGTTGGAATGCTTACTAGTATGAAGGTCTAAAAGTGATTTTAATTTATCAAATGATTCTTTCTTTGCAAAACACTTGTATGCTAGAACTAGTTCGCTGTTAATATCATTTATGCAAAAATTCTTAGGCAAGAGACTAAAAAGTAATGAACCCCCGCCAATAAATGGTTCATAGTAGTTATTAAATTTTTTAGGTATAAAATTTAATAATTTTTCTAAAATTTGTGTCTTTCCACCTGCTCATTTTACAAAAGGTTTTATCACTTTTTTCCTTCCTTAAAATTAATTATATCTTTTAAATTCAAAATTAACTTCATCATTATTATTTACTAAATTTAATGAGTAGATTAGATTCCTTGTAGTTTGCTTTTCTGTATCGTATCATTCCATTGTTTTTCTAAAATTTGTTACATTGCGTTTGTTATTTTGTTGGTGGGTTCAATATCCGTTACTTCCAAAGGTTAAAAAATAAGAATTGTATAAATCAATAATTTCGTTAATTAATAATTTTGCTTTGGGCGAGTTAGTATTTTTTATTCCTTCTATCAATTGCACAACTTGTTCATACAATGATTTATTTTCGTCATATTCAACGTTTGCAAATAATGATAATTCTTTCATTTTTTTATCTAAAATATTTTTGTTTGAAACAATGCTTGATATTTCATCTGATTCAATTTTATTATTATTATTTTTATCAACTCAAAGTTTTAGATTTAAATAGTTATTTTGATTTGTATAATCTTTTATTTCATTAATTCCAATTGAATCAGAAAAATATGAAACATATTCATAATTTTTAACATTATTTGAATATATGCTTTCTTCATTTAGCAGATTCTTTGAATAAATTTCATTTCATTTGCTTTTGGCAATAAAGTTGTATTTTGAAGTATGAATTCTAAGTTTTAAGGGATTTTTATTTTCTTTTTGAAATAGTAAATAGGTTGGATATTTTGCTTCCTTGCTATTTGAATCTATTAATGAATTTTCTAAGTTTAGAAATCCCCCAATATTTATATTTGAATATTGAGAGTTAATATTGCCATTATGATCAATATGTCAACTACTAATATCAGATCCCATATAACTAATAGGTTGACCAAAACCCATTAAATCAATATACGCTTTTAATAACGCCTTTACTTGTTGATTTGGTTTATAAATGTATTTTCCTTGCTTATTTCTAAACAAGAATCTATTATTAATATTTTTATAATTTTTTAATTCATCATCAAATACTCAATTGGCTGAATATATTACATATTTATTATAATAATTGGCAATCATTTTCAATGTGTCATCGCCAATTGATGATAGAAAAATATCATCATTTTTTAATTTATAATATAAGAAATTTAAAGAATCATCCAAAGTTAAATGACCGTCAAACATTTGAAAATTTTCATGTGCTGAATATGAAAGTTTTACTAGTTCTCTTGCAAAAAGTTCCTCAAACGAAAACAAGTATTTTATTTGATCAATATTAGTAGAATTTGCATAATAAACTGTATTGTTTGTTGAATTGTTAAAATAAAAGTTTTTATTGTTTAAAATATTGTATTTTTCAACTTCTTTGTTTGTTAAGGTGAGGTTAGCTAACTTGAATAAGTCATATTGACTAAATTTTGAATAAACTGGTATTTCTTTGTTTTTAGGATTAAATAATTCTTTATTGACTAAATATTCATCGTTTTGCATTGAACCATCGTAGTTTAGCGTTTTTCTAAATTCGGTTAAAAATTTGTTGTTGTTGCCATTGAATTTATTGATTATTTTGTTTAGTTCAGGAGTTGCTAATTTATAGCTCAAATCATTATTGGCAAATCTATCGTTTTCTCTTATACTTCGGTTGTATGTATTATCAATGTGGTGTGCGTATTCATGAAATAAAGTATTAAAAACTAGCTCAACTCTCTTTTGTAAACTTCATGATTGGACTTGTTTTATTCCTCCAGAGATAAAATGATTTATTTGAATAAAAATGTCAAAAGCTTGTGGCAAATAAACACCATTTACATCTTTAATATTCGAAACATTAGGTATTTTTTTATTTATATAAACATGATTTAAACCATTAATTTCAGGACCATAAAAGCCTCTTTTTAAAAACTCTAAATTTAATAATTTTCTTCCTTCTTCACCCAAAAAATAAATAGGTTCATTATTTTTTGTTAAAGCAAAAGGGTATTCAAAAATACTTAAATTTCCATATTGATAATTTTTGTTCTTTAATTGAACATTGTTTTTGACAATCGTTTTTGCATTGAAACTTTGTAGATATCTTTCTTCATTTGGCATTGAATAATTTTTATTTTTTTGGCCATCAAATAAACCCCCGCCATTGCCATTTGAATAATTAACATAATTAAAAATGTAATATATTACGACGCCAATAATACCTGCTGTTAATATAAAAGCCAAGATTATAATAAATATTGCCCATTTCGGTAGAAATGCTTTTCTACCTTGTTTTTGCTTGCTCATTACTAAACTACACTCCTAACTAATTTTAATAACTAATATTTTATAATTTTAATATTATAATTAAATAATATGTTGAGAATAATAGCAGGAAAATATAGATCCAGAATTCTAAAGCAACCTTCCAAATCAATAACAAGACCAACAATAGATAGAGCGAGGGAGGCAATATTTTCTTCAATTCAGTTTAACATTGAAAATAAGAAAGTTTTAGATTTATTTTCGGGATCGGGAGCATTTTGTTTAGAAGCTCTATCAAGGGGCGCAAAATTCGCGGTATGTGTTGATAAAAATGCCGAGGCTATTAAAATAATTAAAGAAAATCAAAATAGTTTAAAAGAACCAAATTTAAAAATTTTTAATATTGATGCATTGAATTTTTTAAATAATAATGTTGAAGTATTTGACTATATTTATTTAGATCCCCCATTTGTTGAAAAAAAATTACTAGTTGAATGTTTAAATAAAATTATTGATAAAAAATGTATTGATAAAAGTGGGACAATGATAATTGAAACAGATTGAGAAGACTTCAATTATAGCTATTGCGGTTTTGAAATAACTAAAAGCAAAAAATATGGTAAAATTTATTTCTACTATATTAGCAATATAGATGATATGGATTAATGAATATTAAACATTTTAGAATCTTATCAACTTAAGATTAGGAGTTTATATATGAGTAAAAAATTAATAATATTTACAGGACCAAGTGGCGTTGGAAAAGGAACAATTGAAAAGCCCTTATTTGAAGATAAAAGTCTAAAGCTAAAATTATCAGTATCAATGACAACGAGATCACCACGTGAAGGAGAAATTGATGGTGTACACTATTACTTTGTGTCAAAAGATACTTTTAACTCTTGCTTAGCAGAAAATAAGCTAATAGAATATTCAATGCACTTTGATAATTATTATGGAACATTATATTCAGAAATTGAACGTATTAGTGAACAAGGAAAAATTCCATTTCTTGAAATTGAAACTAATGGAGCTAAACAAATTTTAGAATATTATAAAAAACGTAATAGATTAGATGAAATTTGTTCAATATTTTTAATGCCTCCTTCATATGGAGAATTAAGAAAAAGAATTGAAGGTCGCAATACCGAAACTGAAGATCTAATTATTAAAAGAATGAAGAAAGCAAAAGAAGAAATAGGCTATTCTACAATATTTCATCATGTGATAGTTAATGATGATTTAAACGTAGCACTTGAAGAAATCAAAGAAATAATCAGATCAGAATTTTCAGATATTATAAACAAAAAAGAAGTTGCAAGTGAAGGTAGAAAGGCATAATTAATGGTAGACTTTTGCATAGCGTCAGATCAAGGAAAGTTTAGAAAAGAAAACCAAGATAGAGCCATTGTTGTTAATGGAGAATATTGAACATTGGCTGCTTTGTGTGATGGTATGGGTGGACATGTTGGCGGTAGCCAATGTTCAACATTATCAACTAATAGTATTAAGCAATATTTCCTAGATACATTTCCTCAAAAGTTGGAATGTAGTGATAAAAAAAACGTTAGCAAATGATTTAATAATGCAATAAGCTTTATAAAAGAAGCTTTAGTTAATTATGCAAAAGAGTATACTGAATTTGAAGATATGGGGACTACAATGGTTGTTGCCCTCATTTTTAATGCAAATGGTTTAGCGTATGTCTTTAATATTGGCGATTCACGCTTGTATGCATACAATGGATTACTTTATCAAATCACAGAAGACCAGAATTATTTATATCAATTGATGAGAGAATTTAATTTAACATACGAAGAAGCAGCATTAGATCCTAATTCTTACAAACTTATAAGCTGCCTAGGCCCTAATAAAAAAACCAATTGTCAATCATTTTTTATATCACAAAAATCAGCAGTTAAATATTATTTATTAACATCCGATGGATTACACGATTATGTTTCTAAACCAATAATAGAAACTGTTTTGCAAACAAATAAGAGTTTAAAAGATAAGTTAAACCTTCTAATAAAATATGCCAAAAAAAATCTTTCAAAAGACAATATAACCGGAATTCTTGTGGGGTTGAAAAATGAATAAGATAAAAGAATTACCCGGCATGTTTGTACTTAATAAGTATTTTACTGATTTTGAATTAATTGGGACTGGAGGATTTGGCGAAGTTTATTCTGCTACATATAAAAAAGATAATAAAAGAGTTGCAATTAAAATACTAAATTGTATCGATGATAGCCGCTGGACAGAGCAAAGAACGAGATTCATTAACGAAATAAATATATTAAAGAAAATTCATAGTTCTAATGTAGTAAAGATATTAGGCTGGCACCATAATGGAAACGAAGTTTTTTATGTAATGGAGTTAATTAAAGGCCAAGGTTTAAAAACAATAATTGCAAAAAACAAAAAAATTGATCCGGATACTGCAATTATGTACGCAAAGCAAATATGTCAAGGTTTGATTGAAATCCATGCTGCCAAAATAGTTCATAGAGATTTGAAGCCAAGCAATATAATAATTGACGAAGAAACAAATAGCCTAAAATTAATTGATTTTGGTATTTCAATAGGTGAATCAACTCAGCGTGTAACTAGAAATTATAAAACTGTCGGTTCTGTTCAATATATGGCGCCTGAATTATTGCGGGGAATTGCTGATCCTTCTCCAAGAAGTGATATATATGCATTTGGTATAATTTTGTTTGAAATGCTTGAAGGAAAGGTTCCTTTTAATAATCATGATGATATTAACCAAATTATGATGATGCACATCAATAATGAATTGCCTAGAATGAGTGATGTCAATGTTATTATTCCGCAAGCATTAGAAAATATTGTAATTCGTTGCACTGCAAAATCTGCAGAGCAAAGATATACTGATTGCATTGAACTAATGCATGACTTAGAGACATGTTTAGATTCAAAAAGAGTAAATGAAAAAAGATTGGAATTAAATCCAAGCAAGTCAAAAATGAAAGCCAAAAGAATCTTTAATAGTAAATTATTCAATATTATTTTTTTCACAACAGGAGGCTTTTTAGTAGCCTTTATTGTGATATTCGTGGTTTTATTTTTAAAGGGGATATTGTAGATGATAGAAGGTAAAATAGTTAAATCAATTGCTGGTTTTTATGATATAAAAAGTTTTGAAGATAAACAAATTTATCGTGTAAGAGGAGGTGGCAAACTTCGCTTACTTGATATAGTGCCAATTGTGGGCGACAACGTTTTGTTTAAAAAAGACGAATTCATCTATCAAATTTTAGGTCGCAAGAATTTTTTTATTAGACCTAAAATTGCAAATGTTGATCAAGCAATTGTTGTGATGTCGTTGGTTGAACCTGAATTTAGTTCATTGCTCGTTGATAAATTTTTGATTATTATTGAAAGTAAGAATGTTAAACCTATCATTGTATTAACAAAAAAAGATCTAACTTCTGATTCAAAAATTGCTTTTTATAAAGATCAAGGTTATGAAGTTTTTGAAATTAATTACGAAACAGGCCAAGGATTTGAAGGCTTAGGAGAAATATTTAAACATAAGACAAGCTTCTTTGTTGGTCAAACAGGTGTAGGAAAAACTACCTTGATAAATTATTTGGCAAAAACAAATTTTGAAACGCAAGCAATTTCTAAAGCCTTAAACCGTGGAAAGCATACAACAAGAGAAGTTAGTTTGATTGATTTTAATGGTGGTGAAATAATTGATACACCAGGTTTTTCTTCCATAGAATTTGATTTAACAATTGAAGAACTACCAATTGCTTTTAAATCGTTTAGAGAAGCTGCAAGCAAATGTAAATTTAGAACAAGTTGTAAACATTATTTAGAATCCGTTGAGGATTGTGAAGTTAAAAAATTAGTTTTACAAGGTAAAATTAAACAAGAAAGATATGATAATTACATTAGTTTTTTAAAAAGACTAACAAATCCAATCAAATAGGAGAAACTATGAAAAAAATAAGTCCATCAGTTTTAGATGTTGAAAAGAATAAATTAACAAACTATATTGACCAATTAGTTGATTGAGGTGTTTGCAATGTTCATTATGATGTAATGGATGGAATTTTTGTAAACAATACTGCTTTAACATTTCAAGAAATCAAACAAGTTAAAGAAAATTGCAAGAAACATACAATGGATATTCACTTGATGGTTAAAGATGTTTTCAAATATTATGAAATGTACAAGAATATTGGAGATATATTGACATTTCATTATGAAGCGTTTGAAAATAGCGATCTTGAAAAGATGATTAAACAATGCCAAAAAGATAACGTTAAACTTGGCTTAGCAATAAAACCTAATACACAAGTTGAAGAAATAAGACAATATTTAAAACATTGTTCTTTGGTTTTAATAATGAGTGTTGAACCAGGCTTTGGTGGTCAAAAATTTATTGAAAATTCATTTAATAAAATAAAAGAATTAGTAGACATTCGTAAGGCTTTAAATTTAAACTTTATTATTGAAGTGGATGGTGGAGTTAAAGACCAAAATATTGCTAAATGTTTTGAATGTGGCGTTAATTTAGCAGTAGTTGGTTCTTATTTAGTAAAAAACTTTTCAAAAGAAACAATTGACCAACTTTTAAAATAATTATAATGAAAAAAGCAAATAAAATATTACTGTCTACATTTGCAACTGCAATTTTAGCATTGCCCTTTGCCACAATTTCTTGTAAAGTCGATCAAGTTAAAGAAAAAGAAAAATTAATAAACAATTCTAGTCAGTTAAATAAAGAAAACAAAAAAATATTATTAAATGAATTAAACATATTGAAAACCCGTTTAGTAAATTCAAAACTTAATGAGAAACAAAAAAACGAATTTATTGAAATTCAACTATTTTTAAAATTAATAACACTTAAAGAACTAAAAATATCTAACGAATTATTAAAATATTATAAATTAATGGCCTTGCTTCAAGATAAGTCTGAAGTAAACAAATACGCAGATTTAATCAAAAAATATTCAAAAAAATTTTCTGATGTTTTGAAGGATGATGGTTCAATATTAAATGATATTTACAATCAAGTTGCAAATTTAATTCCTAGAATTAATTTGCTTAAAAATTCTTATATTTTTTCATTAAATATAAATAATAAAAAATTCACTTTTTTAAGTAATAATGGAATACTATTTAATTTAGATTTTATATTGAATAACATCGAAAACAAGGAAAACAAATATTCAATTTATTACAATACAAAGAAATATGAACTAAATTTAGAAATAAAAAACATTGAATTTAATTCAAATATTAAAACTGCATTAGATTTTAAGAATCAAGATTTTTTAACAACTAATTTTTTGGATTATGATAAAGATTTAGTGTGCAATGAAATGTTAAAAAATGCAATGGTCTATAAAAATTATGAACTAGTTGAAGATTTTGATGAAAATGATAAGAACATTAAATTTTCAAAAATGTTTACTAGTCAAGATTTTGTAAATAATTATTTGGTTAATTGATTAACAGACGAACCAAATATATGCCAAATCGGTGAAGATAAATTTTTAATTTATTACCATTTACCTTTTTCAATAGAAAATAAAAAAATTGTTCAAAAGCCTTCATTGTATATTTCAAATGTTGTTGCTGATTCAGCAATTATTAAAGCAATAGCAAGCAGGAATGTATATTTATTAAAAAACTTATTTTTGTTGTTGCCAAAAGAAAATTACAATGAAAATTTAAAAGTCTTAAATAACTTAATGAAATAAGCATTAGAAAATTAGACTAACAATTAAAATACTGGTCTAATTTAGCTTTGTTAAAAGTTTATGCAAAGTTTGAATTGAAAGTATTGATATGTCATTGCTAAAAGAATCAAATTAAACAAGATAATTATTAAAAACGCTTATCTTGTTTAATTTGATTTTTATATTGAAATAAAAAATTTTAAACAAAGGCAATGTCAAAAATATAGTAAGCAAAGTAACTATATAATTAAAATGTATAAAAAAGTGCTAAAATTTAAACAAATTTTTTGAATAAATTAAAATAATATGATTAATAAAGAATAATTATTTTTTGCTTGCGCCTGCAATTTTTAACTAACTTTTTAAATTGATTGTTAAGGTTCACAATTTTAAAGCAAATTAGAAAGGAAAAAAATGTATAAGAAAACAAATAGAAAAATATTGATTTCTCTTGCCATATTATGTGGAGGTTTAGCAGTGGCATCAATTGCTACAGTTGCTGCATATGCAGGTAAATCAAAACCAGAATCAAAAATAAAAAAAGAACATCTTTTGAATGAACTTAAAGGGGCATTAAATCAATTAAAACAATTGATTGCATCACCGGAAGCTAAAAATATTGAAAAATCAATGGAAATCAATATCTTAAATAATGCTAATGTTTCAAAGGATTCCTCAATTAAAGAAATTCAATCTAAAATCAATCAAGTTAAATATGCTATTGATTCATTAAACAAAAAAATCGCAGATCGAAATAAAAAAGAATTTGAAAAATTCAAACAAATCAAAAACGATCTTCAAACTTATATTAATAGAGATTTAAATGTTGTTATATACAATGAAATAACTAAAAAATCTAAAAATGAAATATTGAAATTAAATAGCATATCGCAAAGCTCAAAAGAACAAGAAATTATAGATGCAATAAGCAAATTGTATGATTTAAAGAAATGAATTTTAGCCCAAAAATTATTAATAGATAATTCAATAAATGAACAAAAAAGACAACTTAAGTCCTTAATTAAAATTGCTAATCAAATATTGTTATCAAACAAAATAGTTGAACGTGACTTAGAACTTAAACAAGAAATTTCTAAGGCTGAGGCATCATTAAGTCAAACAAATAAAACTGCATCTAAACTTTTTAGAGAAAGAATATTGCTTCACAAATTTATTAATGAAACTCCATTATTTGAAGAAAAAATGCTTAAAGAAAAAGAAACAAAAATTAACGAGCTTAAGAAACAATCAGAAACTATTTTTGAACAAATTGGAAAAGACCCAAATAATAGCGAAGACTACCATTATAATTACGATTATAATTCCCAATTTTATAGAAACATAATAACTAAAATTGATAGTTTGAACGCTAATGAAAAAAGTTTAACCTTAAATAAAAAGATTGAAGATATAAGCAATGAAATAAATAATCTAGACAAATCATTTTCTAAGTTAGTTTCTGATACCCTTGAAAAATTTGAATATGTAGATGATCTTAAAGACCATATTGATGTTGCAAAAGATCCCAAAAATTGAATTAACATTCAAAAATATATCTGATACATTAGGACAATAAGCCAAGTTAAAAAACTAAACTTAAAATCACTAGTAGATTTTTATAGCAATGATCAATTTGCTAGATTTATTTGCAATACAATTTCAGACTTTTTTGATTATACAAGAGCAAAGAAAGATTTTGATTTTACAAATTCTGTTATTGCAAATTATGTACTTAATAAAATTATTAAGATCAGGGATTTTGAAGAATTTAAAAACTTAAATAGTTATTTCGCAGATAAAAAAAATGATATTTGAGCAATGCAAGTAGACCAAATAACAGACGAAATGAAAACAAAAATCTTTGATTTGTTAATTAAAGATTTATCTAATATTTTAAAGAAAGATAGTTTTGAAAGCAAACTATTAAGTGCAATATTATTGCCTACAATATTTAAAAAGACATATGAAAATCTTTCTAAAATAATAAAGCCAATTATAGATATGATATATAATAAAGACGCATATAACATAGAAATTATTGGATGAGAAAAGGCTTCACAAGAATTAAGTCTGGATATATCTAATATAAAAGAAGCAGAAAAAAAGGCTTTAGAAATAAAAGCAAAATTAACTCAAGAAAATTTGAAAAATAAATTAAAAGAATTCAAATTAGTAAAAGAAAACTTGGGAAATTTAATTAAAAAACTATCCGAAAAAGGCATCGAAGTCGAAGAAGATACAAAATTCATATATGAAAATTCTGAAGAAGACGAAAATTCTACATTATATGAAGTAATAAATTCTATTATTAGAATAAAAAAAGAAATAAGATACCTTGAATATAAACTAGATTAATAACCACTAAAACATAATAAAAAAATTCCTTTTGATAGCATTAAACCTGGGCAAAAAAATTAGGCTCGCAATGAAAAACGGGTCTAATTTTTTTGTACAGATTTAATTTTTGCTATATATTTATTATTTTCTAGGAATTTTTTGACCTTTAAAGGTAAATCTTAAATTTATATTTCCTTTACAATTTTTATGTCAATACCAATATCATTTCAATTTCAAGGAGTATTGTTCTTATTTCCTTTATAGTAAACTTCTTTTAAAGAATGATAGAAGGGCATTCAGTAATAATTTTGAAATCCAGCTAAAAACGCATTAGTTCCAATTTCTTTAACTGAATTTGGAATAGTTACTACTTCTAGACTTCGGTTTCTAAAAGCATAGCTACCAATTCTTTCAATTCCTTCATTTAAAACTACTTCTCTTAGATTTTCGAAATCCGCAAATGCGCCTTCTTCAATTTCTTTAACTGAATTTGGAATAATTACTTTTTTACTATTTTCAAAGGCTTTTGCTATGCCACAAGAAATGTATGTTATATTGCTATCTAAGATTATATTGCAATCTGAATTAAACATAGCTCGACCAAAACTAACAATATTTTTTGGCAAAACAATTGATGAAATACTAGTTCCTAAAAAAGCATTATCGCCAATAGATTTTAATCCTTCATTTAATTTAACTTCTTTAAGTTCTTTAAAATCCTTAAATGCGCCTTCTTCAATTTCTTTAACTGAAGTTGGAATAGTTATTGAATTTTGATATTGAAGAGCCCCTGTTATATTTCTATTAATTTTTTCAAAGCCATTACATAAAGCAATCTTACAGTCTTTATTAAATATATCTTTAGATAGTTGAGTTACTGAACTAGGAATAGTAACAGAAGAAATTTTAGTGCCTAAGAATGCTTGGCTTCCAATACTATCAAGTCCATCATTTAAAGTTAATTCTTTTAAATTATTGCAATAATAAAATGCATTAGCATAAATATTTTTTAAACTATTAGGAAAAATAATACTAGTAACTCCTGTGCCTTCAAATGCACCTTCTCCAATACTATTAAGTCCATCATTTAAAGTTAATTCTTTTAAATTATCGCAATAATAAAATGCATTAGCATAAACATATTTTAAACTATTAGGAAAAATAATACTAGTAACTCCTGTGCCTTCAAAAGCATTTTCGCCAATTCTTTCAATTCCATTGCTAAAATTAATCTTCTTTAAATATCAACAATCCTTAAAGGTTTTTGCCTTAATTTCTTTAATTGAACTAGGAATAGAAATTGAAGAGATTTGTGTGCCTTCAAATGCACCTTCTCCAATATTTTCAACACCATTTTTTAAAGTTAATTCCTTTAGATTTTTAAAGTTTTTAAAGGCTTCATTTTCTATTGATTTTACATTTTCAGGAATAGTAACAGAGGCAATTTTTCTAAGTCCCTTTGTTAAATTTTTAGTAATTTTGTCAAAACCATTATCAAATTTTATTTTGCAATTTTCAAAAGAAGCATTTTCATCTATTTCCTCTACCTTTGTGCCTTTAAAATAACTAGGTATAACAACATCGATTTGTTTGCCATTATAATTGGTAATTTTTCAATTATTTGAAGAATTTCTTTCAAAATCAAAAAGTTCATATAATTCTTTTTCTTTATTATCTTGAACATTTTGATTTTCGTTATTTATAGAAGAATCTTCATTCTTTAACGATTCTGGCTTTTTGTCATTAGGTTTGTTGTCATTATTATTTGAAGGATTATTATCTATATTTTGATTGTTTGAATTGCTGTCTGTTGTTTTAGTACATGATGCCGCTACTAATGGAAAGCAAATTGTTGAAGTTGTAACAAGTCCTAAAGTTAATAACATTTTTGTTCATTTTTTCATATTTAGTTCTCCTTATTTTCTTCGTCATCTTTAAAAATTGATTTTGAATCATATTTGTATATATCAAAAATTATTTTTAATATTTGTCTTGTTCTAGCTAGGATTTTTGGGTAATATTCTTCTAAATGCAATTTAGAGGCTTCTTCATCTTCTTTTAATTTATCTTTTTTTTCTTTTCTTTCTTTATTATCTTTAATATTTTCAATTTCTTTTCTTCTATTTTTAAATTCTTCTGATTTACCAATTCAATCTTTAATTCATTCTTTTATATTTATTAAATATGGAGCTTTGCCTGAAAAAGAAATATTTTTTTCAATGCTTGAATTTTTATAATCTTCATATTTGTCTTCTAATCGTTTATTAGAAGCAATTTTATTTGAATAGTCATCTAACTTAATTGCATTGCCAATCATATCAATATATTTTTTATATTCATCTTTTTTAATTTCGTCTTTTGCAAAAAGCACACTATTTTGTGCAAGGCAATGTTCGTATTCATTCTTATTAATTTTGTCAATAAATATTGAAAATTCAGAACCTAGATTTAAAGATATTTTTTTATTAGTTATTTCTCATCTATTATCTTTATATTCACCAAGTGATAAGAAAAAGTGAATTCTTGATAGTAATAAAGATTTAGTTTTTGTTTCTAAATTTGCAGGATTTTTTAAGTGGCCGTTTAATTTTTCATTGAATGATTTATCAATTTCGGAATCAGTACTTTGTTTAAGCCCTCTTATTATCCTTTTTAATTCTTCTTTCAAATTATCTGCTGTTTCTATAGTTGAATTCTTATTGATATTATCTATAATGCCAGAAATTGTTGGACGTAATGATTGACCTTCAAAAGCTACTATCTTCCACATAAAAATAAATCTTTCTATTTCAAATAATCATTTAAACAATTCATTAAAATTAATATTGTTTATTTTTTCTTCATTAAACATATTAAATTTTTTCAATATTGTTCATACCAAAGGAATAAAAACAGTATTTTCTTGTGTAGCTGCATAAATTTGCTCTGCCACTATAGAATATGAAAAAATGTCAATACTTGATAATTTATTAACATTGCTTTTTGTTTTATCATCTTTTAGGAATTTTTTAGAATTCTCTAAATATTTATATATGTAAAGCTTATTAATAAGTTTATTAAAAACATTTTCCACTAAATTATTTTCCGAGCTAAGCTGATAATCAAATCATTTTTTTAGAAGTTCATAATTTCCGACGCTTATTAAAGAAGTGCGATTATTACTTTCAATTAAATAATCAATTTTATTTATTACATATGCAAAATTTTCCACCTTTGATTCACTAATTTCTTTATTGCTTTCATTTACCATTTGATCGTAAAAAACATTATTAAAAGTTTTTATGTTTTTTTCATTTTCTCTATCTAGAGAATATAAATAATTTCGAAATAAATCTAGTGAACTTAGTGGTTTTGAATTTAAATTAAGATTTCTAAAAATTATTTCTGAAGAGTCTGTATTAATGTTTGTGTATGTGATGTATGCTTCATCTAGGATAAATCCTATTTTTTGTGCTATTTCATCAAATTTTATATTTATTGGTAGTTTATTAAAAATTATTGTTAGAGTTTTAATAAAGAATTTGTTTTCCTCGTCAACATTTATTTTAAGATTTTCCTTAATAACCTCATTTATAAATTTATATATATTAGCTTCTTCTAGTTGGTCAATATTTTCGTATATCTTTAAATATTCTTTTTTTACAAATATGTTATATAAAAGATATAAGTTTTTATCTTTAATTTCAATCTTCTTCTTGTCTTTTAAAAAAATGTAGCAATATATTTTAAAGAAGCAAAATAAGATTAAAAGAATAGTAAAACTTCTTTGTTGTCCATCAACTATGTCAATTTTTGTGCTTCGATCTGAGTACATGTGAGATGTTACAATAATATTGTTTAAATAAATTGGAATTTTATTTTCAATTTTATAATTAATCAGACTATCGATTAAATTTTCTATTAAGGTTTTATCTCATACATATTTTCTTTGAAATATAGGAATTTGAACATATGCAAAACGTTTTAAAAAGTCATATATTGTTAATTCTTGACTTGATATTTGATTATCTTTTAATTCAACTAATTTTTTTGTTTCATTTGAATTTGGTTTATTTTCCTCAAATTTAATTTCAAACTCATCATCGTCTTTAATTTCGATTGCCATAATTTCTTCAATATTTTCATCAGGCAAATTGCAAATCGTTTTTATTGGTTTTACTATTGAACAAAAAATATCTTTCATTATTGATTGTCATTTATCGTCACAATCCTTCGAAATTTCTTTTCATAATGGTGCAAAATATGTTTTACAACTTGATTTGTTGCCTTTTGCAGAAACATATTCAGAACTAATTTTTTTAATTTCTTTTTTTATTTTTTCTTTGTAATCATTGTCATTAAAAAATTCATCCATTGAAATAAAAAACGAATTAGATATTTCAATATCATTAATAGAGTTTATGCTATTAATTAGCCTTTCTTTTTGCTCTTCTTCATTGTAATCAAAATTTTGTGACTCAATATTCATTTTCTCTCTAAGAATGTCCATTTTATTTTTTAGTTCTTGCAAAATAAAATTGTTTAATTTCTTTTTATTGTCAATATTAACAAAATCTTGTGTTAATTCGTATTCACAATCACTCTTTTTAAATAAAATATTTAAATATTCAGCAATTCTTAAAAACTGATTAATAGTTGAATCAGCAGTGTCGTTATAAATAAATTCTTGGCAAAAGTCAAAGCGTAAGTTAAATTTCTTCTCATTTTTTGAACTTTTTTCAATTATTAGTTTTTTATTTTTTGATATTTCTTCTAAAAAATTGTCTATTTCGAATCATTCAACAATTCTGCCATAATAATCTTTTAGGCTTGTGCCTATTGTTCAGGTTTTAAATTCCATATTTATTTTCCTCATTCAATATGTATTGATTCTTTCATGCATTCAAATATTTGATTTAAAATGTCGGCAATTAAGTTTAGTGGTATTGAACTTCTACGTGAATATGAACCCCTTGCCATATGGTCATTATTTCCTTTTTGTCTTGTTTTCAATAAATTCAATTCAATATTAGATTTAAAAATAGTTGGTTTTAGACTAAAATTTTGATTATAAGAAGAGTAGTATGTGCTATTTTCAAACCCCTCAAAATTTCAATGATTTCTTTGATATTCTCAACTTTTAGAGGTTTTAGGGTTTTCTATTACCCAAACTTTTGGTTTAAATGTTTGAATAATAAAAATAGTTCCGGATATTGTGCCTTCCCCAATTAATCTGCCTCTTTCTTTTTGTTCAAAAAATCTATGTTTTACTGGGTGGCAAGTCTTGTTATATTCATCATAATATTTTTTATTCATAACAACTCATTTGCCATCTTTGGAAATAGAGCGTAGCATTTTTCCGCCACAATCTGCACTTGACCAACTTTCACAAGGTGGCGATGCTAAAATAATGTCAGGTTTAGGTAGTTGCTTTAATTCTTTAATCAAGTTAAAGTTTTGCAAAGATAGGTCAATTTTGTGATAAAAAAATCTCTCTTTGTCTTTAAAAGAGATGTCATTTATTCCAATTGAATGCACTTCTATATTATTAAAATTCTTATAGTATTTTTTAACAGCATTTTTGTATGATGACTCCGCATCATCAAAAAGAGCTCAAATAATTATTTTACTTTTTGGCGAAGAGCTCTCTCTCTCTCTCTCTCCATACTATTACTATTCATATATATTTCCCCAATCTATGTATGAACTACTTCTAATTACTTTATTCTAATAATTATACCTATTTTTAGTGGCTTTCTAGTGCTATTTATATTTAAAAATATTATTAATTTTTATTAGTTATAAAAAATATAAACAATTTTTTTGCTTTATTTTTTATATCCAAAAACATACTCTATTATATAAAGATACATGGAAATAAAAAAACTTAAAAATTTTTAGCACTCTTATGCTTTTAGTGCTAAAATATTAAATGTAAATTGAAAAAATTCAAATTAAAAAATATATTTTAAAAGGAGATTAATTATGGCTAAAGAATTTATCTTAGGTATAGACTTAGGTACTACTAACTCGGTAGTATCTGTTATAGAAAATGGAACCCCAAAAATATTAGAAAATCCAAATGGTAAAAGAACTACACCATCTGTTGTTGCTTTTAAAAATGGAGAAACAATTATTGGTGAAAGTGCTAAAAGACAATTGGAATCAAATAAAGATAGTGTTGCATCTATAAAAAGATTAATGGGTACTTCTCAAACAGTACATCTAAATAATAAAGATTACAAACCTGAAGAAATCAGCGCAATGATTCTAAGTTACATGAAAGATTATGCTGACAAAAAATTAGGTCAACCTGTTAAAAAAGCAGTTATCACAGTCCCAGCATATTTTGACAATGCACAACGTGAAGCAACAAAAAACGCAGGAATTATTGCTGGCCTTGATGTTGTAAGAATTATTAATGAACCTACTGCTGCCGCATTGGCATTTGGATTGAATAAAGATAAAAATGAAAATCAAAAGATCTTAGTATTTGACTTAGGTGGTGGTACATTTGACGTTTCGTTGCTTGAAATGGAAAGTGGAACATTTGAAGTTTTAGCAACAGCAGGCGATAACCACTTAGGTGGTGATGACTGAGACCATGAAATTGTTAAATGAATGGTTGAACAAATTAAATCTAAATACAATTTCGATCCAACAACCGACAAAATGGCAATGGCAAGATTAAAAGAAGAAGCAGAACGTGCAAAAATTACTTTATCAGAACAATTAATTGCAAACATTTCTCTTCCATTCTTAGCAATGAATGAAAATGGACCAGTTAATGTTGAATTAGAAATTACACGTGCTACATTTGAATCAATGACTGAACATTTGCTACAAAGAACAAAAAAACCATTATTAGACGTTTTAAATGAAGCAAAATTAACATGAAATGATATTAATGAAGTATTACTAGTTGGTGGTTCAACAAGAATGCCAGCTGTTCAAAAACTAGTAGCAGAAGTAACTAATAAAAAACCTAATAATTCAATTAACCCAGATGAAGTAGTTAGCGTTGGAGCTGCTATTCAAGGTGCAATATTAGCAGGAGAAATTCAAGATGTTTTACTACTTGATGTTACTCCTTTAACATTAGGTATTGTAGTAGAAGGTGATGTTGTAGCGCCTTTAATTCCAAGAAATACAACAATTCCTGTTACAAAGAGTCAAATATTCTCAACAGCTGTTGATAATCAAACAGCAGTAACAATTGTTATTACACAAGGTGAAAGACAATTGGCTAGAGATAACAAGATTTTGGGTCAATTTAATCTTGAAGGAATTGAACCTGCACCAAGAGGAATTCCTCAAATTGAAGTAAGTTTCTCAATTGACGTAAATGGTATTACTAAAGTAACAGCAAAAGACAAAAAGACAAACAAAGAACAAACAATCACCATTCAAAACACCTCTTCATTATCAAAAGAAGAAGTAGAAAAGATGGTTAAGGACGCAGAAGCAAATAGAGAAGCCGATCAAAAGAAACGCCATGAAATTGAAGTTATTGTAAAAGCAGAACAATTAAGCAATGACTTAGAAAAGACATTAAAATCAGAACAAGCTAAAAATCTAGGCGAACCTCAAAAACAAGAACTTCAAAAAGAAATAGATGAAATTAAAGAATTAATAAATAAAAAAGATATCGAACAACTAGAAAAGAAAATTACAGAATTTGAACAAAAAATGGCTCAAGCTGCTGAATTTTTAAAGAAACAACAAGGAAATAATAATCCAAACACCAACAACGACAACCCTCAAACTAACTAAAAATAATAAATTTAAAATAACGGTTTTAAGCCGTTATTTTTTTGTTTTTTGTCTTTAAAAAAAATTCTTCAAAATAAAAAGCAAAAATGGGACTAAATATTGCATTTTTCCCAAAATAACACTTTTTTTATAACTTGATTAATATTTTTTTTGTAAAATTTCTAACATAATTGAAAATTTTAATAGAAGTTAAAGTTTAAAATAAATGGAGGAAAATAAAAATGAAGACAGCTTTAAACAATTATCACAATTTTTTATTTAAAGTTACTGCTAAGAAAAAAAGCACAATAATTATTCCTATAATTTTATTGTTATGCAGTTTAATTTTGTGTTTTGTTTTTGTTGGTACTAAACCAGCACCGCGTTATTTTAATGTAATAATTTTTGCATATACATTAGTTGCAATATTATTTACAGTATTATATGGCTCTCTCAAGTCCTTAAATATATTTAAAGATCTTGAACAAGATGGGATTGAATTAATTATATTTTCTAAGCCCATTTCAAGAAAAGCAATAATTTGAGGGAAAATTCTAAGTTTTAATTCCCTTGGACTTATTTGAACATTGTTTGCTTTTGTTAGTTCTATTATTGTATATAGTCAAGTTTCAAAAGGAAACATGTTTGGATATTTAGTTTTGTTGGCATTGGTTGCACATTTTCTTGCATATACAATTTTTGGATATATAGCAGCATTGATTGCATATAAAGTTAACCAAAAAATTGCTATAACTGTTCCAATTATAATTTTTGCACCTATGGCAATAGGTGGTGGATTTATATTTGCCAACTCAACATCAACAAATGAAAATTTTGCTCATTATATTAATAGCAAATATAAATACCACAGAGCAGGAAACGAAGTTAATAGTGAAGTATTTTATTTAAATAAAAATGATGATAAATATTATTTAGTTCCAAATGGAATTAATAATAACAAGTTTAGTGATGTACAAAATCAATATTTAAATTTAGCCTGAAAATATTCAAATAGTTCGGCTAACGAATGGCAAAAATATTCATGACTTGCAATGCCATATCAATTTGTTGATATATTCAACATTGAAAATCAAAATATTTTTAGTAACCTTTCTTCAGACAGTATTAATAATAGTTTAAGTAATTATTTATATTATAAAAATCAAGAAAATACAATATATAAATATAAATTAAATAGAAATTCGGACTTATTAAAATTACCAACATTAGAAAATAAAACTAAGATTAATAAATACTATGTCCCAGGGATGTTAAATTTAAATTCAAATATTGATAATGAAGTAAATAATGGAGTAATTTACGCTAGAAAAAATGCTTCTAATTTTTCTGCTTCTTTTGAAGAAGACAACTTTGTTTATGCATCTCCAAATAATCTAGTAGGAAAATTAAAATGAAAAATAATGAGCCAAGTTTTAAAATATCATAAATTCACAGAAGCAGCCGAAGAATTTTATTCTAAGGTTAAAAATGAAATTAGTTTAAAGAAATTAGTTAAACCACATGATATTAAAAAGTTATTGTTAGAAACGATTTCAAATGAAGTTAATAATACAAATAGTAAGTTCTTTAATATAGATAATGTTAACTTAGCAGTATTCGACCCTCATGGTGTTAGAGATGGAAGATTACAATCTGAAACAGAAAGAAAAATTTATATTGCCACAGCATTGCTATATTTTATTTATTTTAAAGATAATCAAGGTCAAGTTCTTAAATCATTATTGAAATCTGAAAGCGATAAAACAACATATGCACCAAGCCAAATAGAAATCAAGGTAGATGGTTATATCTATGAAATAGGTGGATATGAAAGCTACGAACCAAAACAACAAGTGCAAGAAAATAAAATAACTAAAAATAAACAAGTAGTAGTTAGATATGAATTAATTCCAAGCAATAAAAATTATTTATTCCAAACAGTAGATGATGTATATTCAATTGAAAGAGATAGAAAAATAGTTAATAAACAATTTTATATTTTAATTTGAGTAGTAGTGGCAATAATCTTAATGATGGCAAATAGATTTCTATATCTAAGAAAGGATTATAAATAATGGATAATCATATTTTAGAATTAAAAAATGTAGTAAAAATCTATAATAAATCAGATCGTGGAATTCATAATATTTCATTTGCTGTCGAATCTGGAAATTTTCATGCATTTATTGGCGAAAATGGTGCAGGAAAAACAACAACAATTAAAACAATAATTGGTTCTTATATAAATTTTAAAGGTGAAATACTTATTGGTGGAATTACAAACTTAAATCCTAATTCTAAAAAGATTCTTGGTTATGTTCCAGAAAATGCAGTGTTTCCTAAAGAACTAACAACATATGAATATTTAAAGGCCCTTGCATTATTATCGGGGATAAATAAAACCGAAGTAAAGGCAAAAATCGAAAGTTACTTACAAAGATTTGGAATTATTGAATTAAAAAACGAAAAACCCGCTAATTTTTCATCAGGTCAAAAGAAAAAAGTTTTATTAATACAGGCTTTGATTAACGAGCCTAAATTATTGATTTTAGATGAACCTGCTGCTAATTTAGACCCTACTGCAAGATTTGAATTGTTTTCAATATTACATGAACTAAATGAAAAAGGAACAACAATTTTTATTAGTTCCCACGTTCTAAGTGAAATTGATAAATATGCAAATTCGTTGACATTAATTCATGGCGGAAAGATTTTATATTCTGGCCCTAAATATGATTCATTGGAAAAAATATTTTATGAAAAAGTTATTAAAAATTAGTTTGTCATTGCCATTAGTTATTGCAACAATTCCATTAGTTGCTTCAAGCTGCAAACAAACAAATATTGAAATTAAAAAACCAGATGATAAAAAAGAAACAGCAAATCAAATCCAAGCAAAAATTAATAAAGAATTTCAAAAAAATAAAGTGGTTCAAAAATTACTAAGTTCATTTATTGAAAATGAAAAAGAACAAGATATTTTTGTACAAAACCAAAATAATATATTGCCCAATGTATATTCAGAACTAAAGTTTTCTTTGTTGTATTTCCCTATTTATAATAGAGCCTCAGATAATCCGGGTAGCGAACTTTATGGATTTATAAGACAATCAAGAGAAATTATTGAAAACACATTGCAAAGAAATTGATATTGATATTTAAAACAAATTGGTAATTTTGTATATTCAATAAACCCTTATAATGATAGATACATTGAAACCCCTCAAACAAAAAAAACAATTCAATCTGCCCAAAAAGACATTGGCATTCTATATAAAATAAAAGATGCAATGCCTAAAGATATTATTGAATTGCCTTATAACCCAATAAGTTCAGATGAATTAAAAGAAATAGTCGCTAAAAAAATTAATGAAAGTATTGATTCTGAAGTTCAAAACCAATTTAGCGATTATTTAGAAGATAGAATTAATGATTCATCAAAGTTAAAAATAGATACAGCAACAGATATAACCAATTTTTTAAAAAGCAAAATTAACCTAAATGATGCAAAAAAGCTAATAGAAAATTATTTGAACAATAAGAACAAATTTGAAATTAGTAATCAATATCAAATTAATAATGAAATTTCTAAAAAAATATTTAATTTATTAAATACAAAAGGATCTTCATTTGATAACTATTCCTCAAAGAAAATCTTCTACGTAGCGTACGACCACAAAACTTGAATAAGAGTAATGGTTTATACTGAAAATAACGTTAAAAAAATTTCTATAAATCCTGATGTTTATATTTTAAATCAAAGCAATAGCTATGAACAAAGCAAAGAAATTTTAAAAAAATTAAATAAACAAATTTTTATTGAACGGTTGAATTCAATTATTGATTCTTTAATTGAAGACATAAATGAAAAAGATTTCAATATTAAAAACTTTTTTGAAAAAAATAATGATAAAAAATTCTTTGAATTGTTTCAAAAGTTTTCATACAATAAATACTTGATTGAAGCAATGCAAAAAATTAATAAAGAAAAAGATCTAATTTATCGCTATAGTTGGGAGACAGTAGATTATGAATAAAAGAAATTTATTAAATTTTTTGTTTGTTCCCACTTTAATTCTTGCACCATTAACTGCTATATCGTGCGTTGATAAAAGTGAAAAAAGCGATTTGAATGCATTAGCAACTAAAATAGGAGATGTTGTAAACAAAAAAGAAAATCCTGCTATTGAAGTAACCAAAAAAATAATTAACGACTTATTAAAACAAGTATATAGGGATAATGATGTTGAAAAAATTAATTTTTTAGATCAACAACAAAATAAAGAATATCAAAAAAACATTTTTTTGAAAATTAAAGAACTTTCAAAAAAATGAAATTCTAAATTAAGCAATAAAGAAAAAGAAAACATTGCCAATCAATTAAATCAAATTTATGAAAAAAATTGATATATTGTGTTAACAAATATAAATAAATTTAATTTAAGATTCTATGAATGACTTTTGTTTGATAAGCCTGAATTTTTAAGCCAAATTAGGGCAGATGGAAAGCATAGCAAAGAATATTTAAATTCATTAAAAAGCGAAAAGAAACCAGATAATGTTCCGATTATTAATAATTATTTAAAGGAATTAAAAGAAGGTGATGAATCAGGAGAACTTTCAAATATGAACATGCTTTATTTAAAAAAAGATAGTATGATATTCAGATTTAGAGTTGTTGATACATTGACAAAACCAAAAATCAAACTAACGTGTTTTGTGTGATATTTTAGTAAATTTAAAAATATATCAATAAATTTGATTTCAGATATTGTTCATAGCGCTTATATTCACGGCGTTCAAGAAGGATTCAATAGATTTGAAAATGATATGGCAAATCGTTATGGCAAACCTGCCAAAATGATTATGGGAATTTTCGATGATAATGCTTCTAGCGCCAATGGAGGAAAAGAAAATGAATAAAAAAATTTTGATGACTCTATCATTGAGCACGTTAAGCATTATACCTAGCTGTTTTTCTTTAATTTCATGTACTAAAGAAAATAAACAAGATGAAAAAAAATCATTGAATCAAATAATTGAGGGCAACGAAAATGCAAACGAAAGAGAATGAAAAAATTTTTTAAATAGAGATTATGTTAAAGAAATTTTAAGACTTTCGTTTGACGATGATGATGCTAAAAACAAATATATAGCCGAACAAAAAAAATTAAATAATAATATAGAATATTCAAAAAAATTAAAAGAAGCATTGATATATGGAAATTCATTGAAGGCTTCTTCTAGTTACAATAGCGATGATTTTTTAACACAGCCTAAATTATTGTTTCAAAATGGAAAAAAGATTTTTAATGAACTTAAAAGTAAGAATTGACTTTGATTTCTTTACAATCTTCCAAAAATAATTTTTGGTTATTTTCCTCCAATGAATAAATTTGAATCATCAGATGAACAATCAGCATTAACTGCGCAAGGTAATTCCTTGATTTTAGGCCATTTTTATAAACCAAGGAGTAATGAAATTATTCAATATACAACTCAAGTTACAAAAAATGACAAAAATGGTGAGATAACTTATGATGTTTATTTATTAACAAAAGAAGGTTTTATTTTTCAATTAACTATTGATTGAGCAAAAGATGATGAAGATAATAAAATAAAGCCTAGTGTTAAACTTTTTGGTTATATTTACACCTATCCAAACATAGTAAAAGACAGAAATAATTTAAATATTTTCAATATTCAAAAATATGTTATTGCCAATACCGAATCATTTGATGATATTAACAATAGAACATTGCAAATATTATTTCAAGATCAATATGGGGGTAGCCCTTTACGTTATACAATAATTGACGTTAGTTTAAAATAAAATGTTAATAAGATAAAAATTCAAGTGTACACGAATTGAAACAAATTAAGTGTTGACTTGATTTTTTTAAATTAAAATTTCAAATGATATGAGCAATAAATCAATATAAGCATCTTTTTTTTGTTTTTAAAATAAAGTTTTTTAGCATATTGTGAATTTTAAAATTTAAACAATTATTTTTTTATATTTTTTGATACAATTTTATTGTATTTATTACAATCGGTAAATTATTCAATTATATTAATTTATCAAACACTATTACGGATTCATTTAACTAGTGTTAGTAAAGATTACTAATGTTAAATGTTAGAAATAATAGGAAGAACAACAAAAAAACAAAGGAAAATTATGTCAGAACAAAAACAACAAGCACAACCAGCAGTAGCTCCAACACAAGCAGAACCTGCTATTGTATCACGCGAAAAATTATTAGAAGCAGGAACATACTTTGGACACAGAGTAAGCCAATGAAATCCAAAAATGAAACAATATATTTGAGGAAAAAGAATGGGAATTCATATTATTGATATTGCCAAAACTCAAAAAACATTGGAATATGCTTACAAACTATTAAATAAAATGGCTCAAAAACCAATTTCATTTATTTGAGTAGGAACAAAAAAACAAGCTAAAAAGGCTATAGAATCAGCCGCAGCAAGAACAAATTCAGTTTATGTTTCAGAACGTTGATTAGGTGGAACATTAACTAATTCACAAACAATTTTTAGAAGTGTTAGAGAATTAGAAAGATTAGAAGAACTTCAAAAATCAGAATATCAAGGATATACTAAAAAAGAAGGTCTTTTAATGGACAAGAAAATTGCTAAATTGCAAAAGAACTTAGGTGGTATTAGAAAATTAGCTGGAAAAGCAGCAATGCCACAAGTAATGATTTGTGCATCACCTATTGATGATGAAATAGCAATTAGAGAAGCAAAAAAGAAAGGTTTGAAAATTTTTGCAATTCAAGATACAAACTCAAACCCAGATTTAGTTGACTTCGTAATACCAGCAAACGATGATTCTGTAAAGTCAATTACTTTAATTACAACTATCTTGGCAGACGCAATAGCATCAGCACATGGTGAAAAACCAGTATATGCATATAAGTCAAATGAAGAAATTATCTTGCCAGAAGAACAAAAACCAGAAAAGGTTGAAAAAATAGCAAACACCGAAGAAAGAAAACCAAGAACTTTTGTTAAAAAAGCTAACGTTGAATCAGAAAATACTAAGAAATCTGAATAAAATAATTGAAAGGGAATGTTATGTCAGTAGATTTAAAAAAAATTAAGGAATTAAGAGAAAGAACAAATTCAGGATTTTTAGATGTTAAAAATGCATTAGAAGCAACAAACAACGATATTGAAAAAGCAATTGAATGATTGCAAGAAAAAGGTATAGTTAAGGCTGCTAAAAAAGCCGGAAGAATTGCAGCAGAAGGAATAGTAAGAGCTGTTATTAAAGATAATATTGCTGTGATTTTTGAGTTAAATTCAGAAACAGATTTTGTTGCTAAAAATCAATTATTTTTAGACTTTGCTAAATTATTAGAAGAAGAATTGCCAAAAGTTAATTTCTCAAATATCGATGATTTATTAGAACAAAAAATTAATGGTAAAACAATTGAAGAATATACAACAGATTTAACAGCAAAAATTGGCGAAAAGATTCATTTAAGAAGAGCATTAAAGTACGAAGCAAAAGAAGATGAAGTTGTTGCTGGATATACACATGCTAACAATAGAATTGCTACTATTTTAATCGCTAAAGGACATAACCAAGAAGCATTAAGACATGTTGCAATGCATATTGCAGCCTTAAACCCTGCTCATTTATTCGAAACATGTTTGGATGAAGAAGAATTAAAGGAAATTTATGAACGTATTAACAACGATCCTAAATTAGCATCTAAACCAGAAAAAATTCAAGTTTCTATGAAATCAGGAATGTTAAGAAAAGAATTCAATGAACGTGGCGTTTTAATGTATCAACCATTTGTTATGGAAGAATCAAAAACAGTTCAACAATATTTAACAGAAAATAAATTACAATTAATAGATGCAACAAGATTTGAAGTAGGCGAAGGAATAGAAAAGAAAACTGTTGATTTTGCTACAGAAGTTGCTGAACAAATGTCACTTAAATAATAATTAAGTGTTAAAAAAAATAGGTTTTTGCCTATTTTTTTTGATACTTTTTAGTTATTTTTTTTAGTTTAATTAAGAAATGATTCAATGCAGAACGAGACTTTTTAATGTTTTTTGTTTCTAATAATAAACTAAGTTCATTTAAGGAACTGTCTAAACTATTTAATTTTAATTCAAATAGCGCAAGTTCTTCATCGCTAAACTCATCCAAGAGATTGTTTTCTTTTATAAATTGATAATTTTTAATAAATTCTAAATTTGCTTTTGCAATACGTTCTTGATTATATACGTCGAGATTTGTTATTCTATTTGCTGAATTATAAAAATCTCTCTGAATTTTGCCATCTTCGAATAAATAATAGGATTCAATGGTTTCAATAGCTTTTAAAAAATCACAAATATTTTCAATTTTTTTTAAATAAATTATTATTTTTTGTTTTCTAATTATTTTTTTAAATTCTAATCCGTGAGAATTTAAAATTGATAAAGCATTTTCTGCATTTATTAAATCATTAAATTTTAACTCTAGATGATTTGTTCTTCCATTTATTATTGAACCAGAAGAAAGGAATAAACCAGCAAAGAATTTTCCTTGATATTTAACGCTTATGTTAGCTAAAAAATTTGTATTTATTGAAATTTCATTTTTTTTAGTATACGTATAAGAAATTTTTAGTTCATTACAAATATTTAATATATATGTCCTAATCTTAATATTATTTATTACTAATTTAGCTTCATTGTTTTTTACATTACAACAATAAATTATGCCAGCCATCAAAAAAAACTTTTCTTGTTTTTTTAACTGAAAAGACATAATTTCTTCTTTTACTAGTTGACTAAAAGATTTATTCTCCATAGATGATTTCTTTCCTTTAATATATTAAATTATATTATATAGGAGTCTTTATTTAATTTTTATGCCTGCATTAGCCATTAAATTTAAAGCAAAATCGTGATATTCTTGAGAATTATGATTTGGAATATTAAATGTTGCAATTAAATTTTTAAAGACAATAATTTCTTTATTTTGCTTTAAATGATTGAAATAAGTTTTTAGAGTTATTGCAAATTGTAAAATGCAAATATCTGAACAACAACCAATAATTTCAAAGGAATCATACTTGCTTAAAATTTCCTTTTTTAATGCAAAAAAGGAGTTAGTAGAATTCTTTTCAATTATATTTTTAGCATATTTTTTTAATTCATCTACTACTTGACTTTCTTTGCTATTTTTTAAACAATGTATTGGGTATGATTGCATTTCAATATCATCGCTACTATGAGCATCGTTTAAAAAATATATATCATTGTCTTTATATTGAGTTTCTAAAAAATTTTTTATAGGAACAATTATTGACTCAATATTGCAACTATATAGTGCACCTTTTTTACAAAATCCATTAACCATATCAACAACAAAGATTGCTTTCATATTTTTTACTCCTTTTTTTCATTTTTCAATGTTTTTGTATTGTCATATAAATTGTTAATTATTGTATAATTATAACGGATACATCTTAGAGGGACATTTATTTTTAATTATATGTTGGTGTATCTAAAGTTCGAATTTTATTTATTTAATATATAAGAAAGGATTTCTAATGGCCGAAGAACAATTAGTTGTTACTTCAAGTCCTAAGAAAAAGATTGGCTTCTTTTCAGCTATTCTTATCGTTATGGGTTCGGCTATCGGTGTTGGGGTTTTCCTACGTTCAAAGAACGTTTTACAAAACTCAGCTAACAACGTCGTATGAGCATTGATCGTTTGATTAATTGCTGGATTTGCCGTAATTACCATGGGTATTGCATTAGTTGAAGTTGCATCAGGACGTAACGACAACTTAGGTGTTATAGGTTGAACCAAGGCATTTAGTACATTAAACTTATACAAAGGTACTAAGTTCTTTATGACCTATCTATATCTACCATTTACTTACTTCTTTATGCCTTACTACGTTATCGTTCAATTCCAAGACGGTATCGCAGGATTCGTAGGATACAGCAAAGTAGGATTTGGTGGTTCTACAAGTGCTCCATGAATCTACTTTGCAATAGGTCTAGCACTTACATTATGAATGATTTTTTCAGCCGGTATTAGTGGCCGTGCTACAAACATTCAAAACTGAATAGTTACAGTTGCAAAATTAATTCCTATTTTCGTAGTTATTATTTTGGGTATTATTTTTGCTGTATACAAAGGTACAGGAAAAGAAGGATTCGTTGCTGGAGTTGATGATGGTGTTACACCACTATGACACAAAATAACATCAGCTGACCTATTCAACAAGAGTTCATCATCATTCTTAGGATTAACACCATTCCTAACTGTATTTAGCTCACTAGGTGGTATCTTCTTTGCATTCGACGGTTTCTATGTAACCGCTGGTATTCAATCAGAAATGAAACACCCAGAAAAAACTCCTGCTGCTATTGTTATTGGTTTAGCATCAATGACTGTTCTATACATGTTAATTGCTGTTACAATGACATTAGGTGGAAATAACGGTTCATTCTACGGATTCGGAGATGAATTAAACGCTGTTAAAGCAGGTTGAGTATTTGGTGTAATTAACATTTTAATCTCAATCGGTATTATTGGTATTCTAAACGGTTTCACCGCTTGAGCTACAAGATGAGTAGAAGACTTAATTAGAGAAGGTGAAATGTTCATCCCTGTAAAAGCTTACAGATACATTAAACATTCACAAGTTCCATGAGTTGGAGCAATCTTCGTATTATTAATTTCTCTACCATTCATGGTTATCTTCACTGCTATTGGTGCTTACGCATACTTTGGTGGAGGATATTCAACAGACTACGGTTACCAAATTGATAACCTATTAACATTTGCTGACTTGATGGCAAACTGAATGGCTGTATTCGCATTCGCATTCATTGCAACATCAATTATCGGAATGTTACAAAACAGAAAGAAACACTTTATTGCTGTTGTTGAAGGAAAACACAGTAAATGAACAGGATATGTATCAGTTATCTTCATCTTATCAGTTATGTTCTTCTTAGCAATTGACCCATTCATATCAATAGGTCTATCAGCAGCACAATACGTTGCAGATAAAACAGAAGACACCAGAAACGGCTTAGTAGGAAACATTGCTACATCAATTCTATTTATAATATATGCAGCAGTTTCATTCTTAGGCGCTCCAGTTGAAAGACAAATTGCTAAATCTAACAAGATTAAATTTGATAGAGTTCTTTCAGGAAACATTTGTGCACACGAAGTTCCAGAATGATGCCCATGCAAATTAGAAGAATTAAAAGAAAAAGCTGAACTAAATGACATGGTTCTAAAAACATTTGCTGAAGCAAGATAATAATTTGTTATTTCACCAGTGGGTTATTCCCACTGGTTTTTATTTATATTTTTAATATTCAATTAAAAACTTAGTTTTAATGTTAAAATGTAAAGATAATTATTTTATAAAGGCAACCAATGAAAAATAGTAAAGAACAGATTAACAATATTCCTTATGTTTTAAATGAAACATTGATTAACACAAATTTAAAAACTGGCCTAAGTTCACAAGAGGCTGATTTAAGACTTAAAAAGTTTGGAAAAAATGAATTGCATAAAGCAAAAAAAATAAATCCATTTTTAATATATTTAGAGCAATTTAAAGATATATTGGTCATTATATTATTATTTGCAGCAATAATATCTTATGCTCTTGCGATTGTTTCAGGAACTCAAAATAATTGAAATTGATCTATTAATGGTAATCGCTTATTAATTGAATTTATTGAACCTACAATTATATTATTTGTAGTTCTTACTAATTCTCTAGTAGGCGCTATTCAAGAAATTAAGAGCATACAAGCAGTTGAGGCATTGAGCAAATTAAGTCCCTTACAAGCTAAAGTCGTCCGTGATGGCAATTTAATAACGTTAGCATCAAGTGATATAACCGTTGGCGATATTGTTTTAATTGAATCAGGTGATGTTGTACCTTCTGATGGTTATTTGTTGTTTAGTTCAAATTTAACTGTTATTGAGAGTGCATTAACCGGGGAAAGTGAACCGGCGACAAAAGATTACCAAGCTAAAAGAAATTTAGCATTAGCAATGGCAGATAGACAATTTATGGTTTATTCTTCGACTGTGGTTGCTACAGGAACAGCTTATTTTGTTGTTACATCGGTAGGTGAAAATAACGAAATCGGAAAAATTTCTAAACTAGTTAATCAACAAAAAGAATCGATGACTCCTCTTCAAATTAAAATAAATAAATTAGGAAAAATATTTGGATATTCAGGTCTTGCATTATTTTTAGCAAGTTTAGTGACTCAAATAGTTTTCCATGCGGTATCAAGAAATTCTTTTGCTGATACAATGTTTTGAGGCAATGCAATAGTTGGAGCCATTTCACTAGCCGTTGCTGCCATTCCTGAGGGGTTAATTGCTTTTACTTCAATTATTTTAGCCATTGGCGTTCAAAGAATGGCAAGAAAGAATGCAATTGTTAAGAACTTAATGGCTGTTGAGTCTCTTGGCAATTGTGCAGTAATATGTTCAGATAAAACAGGAACATTAACACAAAACAAGATGACAATTGTAGATGCTTTTGATGGAAGCCAACTACTATCAAATAATAGTGAAATTAAAAACTTCTTAAATATTATAGAATTAGGTTCATTGTGTTGCGATGCAAATTTAACTCGCAATGAAAAAGATGAATATGTTGTAGTTGGAGATCCAACAGAAATAGCATTTTTATATGAACTTGAAAAATTCTCTAATTTTAAATACAAAAACAACCTAGAAATAGAATATAAGCGTTTGCATTTGTTCCCATTTGATTCAGATAGAAAAATGAAATCAGTAATAAATCAAAACAACAACAATTATTGAGCAACGGTTATGGGAGCTCCTGAAATAATAATTGAAAAATGCAATTTTGATTCGCCTTCATTAAAAGAAAAATATTTACAAATAAATAATCAATGGGCAAATAATGCTTATAGAGTCTTAGCAATAGCAAGAAAATCCTTAAAAAAGGAAACAATTGAGAAATTCAATGAATCATCAATTTCTAAATTACAAGAGCATGTAGAAAATGATTTAGAAATTGTTGGATTAGTTGCAATGATTGATCCTCCAAGAGAAAGTGCAAAAGATGCTATTACATTATGCAAACAAGCAGGAATAAAACCTGTTATGATTACTGGTGATAATTTAAATACAGCAAAAGCAATAGCAACAAATCTTGGCATATATAGTGATCAAGATCGTGCTATAACAGGAACTGAACTTTCAAACATTTCTGACGAAGAATTAGTTCAAAATATTGAAAAATACAGTGTTTATGCTAGAGTTAAACCAGAAGATAAATTAAGAATTGTTAATGCCTGACAGCAAAGAAAACAAGTTGTAGCAATGACCGGTGATGGTGTTAATGATGCTCCAGCATTGAAAGCTTCAGATATAGGCTGCGCTATGGGCATAACAGGAACTGAAGCTTCAAAACAAGCTGCAAATGTTATATTGGCTGATGATAATTTTGCCACCATAGTTTCAGCAGTAGCAAACGGAAGATCAATTTATCAAAAAATTAAAAACGTAATTCAAAGTTTATTGATTACATCGATTGCAGAGATCATTTTATTATTTATAGGTTTGTTTTTGTTTAAAGCGATTTTTTCAAGTGCTGAATGAAAAGTGGCATTAGGAAAAAGCGATTTATATATAATGTCAGCTACTCAATTATTGTGAATCAATTTATTTACTCATGGTTTTCCAGCAATAGCATTAGGACTACAAGATTCTAAAGAAGACTACATGAATAGAAGGCCTATATCAAAATATGAGTCAATATTTGCAAATCAAATGGGATTCAATACTTTGTGAAAAGGAATTTTGATTGGAATACTATCGTTAATAACTTATTATTTAGCAGCGTTATATGGCAGAAATAATGGATTTAATGCAGAAGATATATTAAAAGCAGCATCTACTTGTTCATTTTTGGTTTTAGGTATATCTGCTACATTCAATTCTATAAACTTAATGACAAAGAAATCAATCTTTATATCAAATCCATTATATTATTGAAAAGTTTATGCCTCAGTAATTTTTTCATTAGCATTTCTATTGATAGTAGTATTTGTAAAAAATATAAATCTAGTGTTTGGATGTTACACCGAAATTCATTCTAAGCCCGAACTTTTAGCACTAGGATTAGCATTACCATTAGTAATTATTCCAATATATTCAATTCATAAATTAATCATATATTTAATTGAAAAAAACAAAATTGAAGAAAATAAAATTGTTGAATTTAAAATGATATTACCTCCAAGGCAAGCCAAACGAAACCAAAAAAGACATTAAAAAAATGTAGATCTATAGAAATCTACATTTTTTATATTGCAATATAAATTATACAATTTCTTTTTGCTTCAATAGCTCCGTTAAAGGCTTACGCCTATTAAAGTAATGAATACAACAAAGGCAAAAAATATAGTAAAGCAAGAAATAAAAATTCCCGCTATTGACAAAGCTTTTCCTGAACCATTCATTGATTTTGACTTGCTAAGTCCAATACAACTAAAAATAAGACCTAATATTGAAAAGAAAAATGACAATATAAATCCAACAATAGCAAATTCATTAGTTACTGAATTTTGGATTTGTTGTTGAGGTTGCAAATAAGGCTGAGATGGTTGCATTGTATTCATTTGTGTTGTTACATATGCATCTTTTTCCACCTTTTTTCCGCAATTTGGGCAATAATTAGCGTTATTTAATTTAGTGCCGCAATTTGGGCAATAATTCATACTACCCCCCCTCTTATTAAAATGCATAAAAATTATTTTTTAAATTAATTTTATATATAAATATAACACAATAAAGCAATAAAAAAACATCCCAATTCTTGTTGGGACGTTTTTTATTTAATTGATTCTTTTTTTGTTTATATATATTTTATTTTCTATATCGAAAGTTTCGCCGTCAAGTTGCATAAAGTGCATTTCCTTACATTCCATGCTAAATTTATATCCTTGAATTTCATGTACATATTTTTTATAACGTAAATGTTTTCCACTATATACCGAAAAGAATAATCATGCCAATCTTCATTTAGAGATGTTATTAATAATGATTAGATTTAATTTATCTGTATTGTTGCAATTAGGGGCTAATTTCATTCCACCCCCAAAATAACAATTATTCATAATTGAAGCTAATCAAACATTTTTAAATTCTAATTCTTTTCCATCTACATTAATTTTTATTAAAGGAATTGTTTTGAATGTCTTAAATGTATTTATTGTTACTTTAGCAAATGACGATTTATTTTTAAGCATTTTTTCATCATTAACTTTTTTTGCAATAACTGCATCAATACCAAAACCAATGCCATTTAAAAAAGATCTTTCATCTTGGTCGTTTTTAAATTTAATCGTTGGTAAGTTGGTAATGTAAGGAGATATCAAATAATATTTTTTATGTATTATTTGAACATCTTGTTTTATTTTTAAAGACCTTAGAAAATCATTACCAGTTCCTGCTTTATATGCATAAATTTTGGGTAAAGGTTTTATATTGTGAATTTTATTAGTGATATTGGTTAATGTTCCATCACCGCCGATTAATAAAACAATATCTTTTTCTATATCCATTTGAGAAACTAGATTTTGACTGTCTTTAATTTTTATAATGTCAAATATTTTTACCTCAGGTTGTAAAAATACTTTAATGGCGTCAGAAACTATGTCATACATCTTTCTTTGACATTTTCCACTCTTTGATAGAGAATTATAAAATATATATAGCATAAATTATTTTTCCAAATCTTGTAATCTATTTAATGCATTGATATAAATTTCAAGTGATGATTTTATTTCATCAAATGTAAAGTATTCGTTTGGCCCATGCATTAAATGCATATATTTAGTTGAACCAAAAGCGACACAAGTTTTTATTAATCTTGCAAATGTACCGCCGCCAATTGCTAAAGGTTTTTCATTTGAATTCATCGCTTCATTATATGTATCCATAAGAATTCTTACAATTGGAGAATCTTGTTCAATATACTTTGGTTCCTTGAACCCTTGCAATTTTGAAAATACATCATCGCCAAATTCATCTTTTAAATAATCATTTAAGCTTTTAGTTACATCATCAATTTTATGAGAAACAGGGACTCTAAAATCAAAAGTTAAAACATATTCATTTTTCAAGGTTCTAATAATACCTAAATTTGCAGATAATTCGCCCGAAAAATCACTATAGTTTTTAAAAATATTACTTAAATTAAATGATTTGCTATTTAAATTTTCTTTAATAAATTTAAATAATTTAGATTTAGCAAATTCTTTATTTTCTTTGCTTGCTATATTAATGGCTTTAATTATTGCATTGTCACCTTTTTCAGGTGTTGAGCCGTGTCCACCAATCCCCTTAATAATATTAGGTTTTTCATTCAATACTAAAGATGTTGAATCAGGAATTTGGTTAACAACGTCGCCCCCATTTAATTCAACTCCTGGAACCTTTGGAAAATGAATTTCTGTTACATAGATCATTTTTTCAGCATATATTAAAGGTCATTCACCATCAGGAGTATAAGAAATATAAGGATCTTTGAAATCAGCCAAATAAAACTTCATTGACTTCATTGTGGTTTCTTCTGAAATTCCAAATATTATTCTTATTTTTCATTCGTTAGATAATAAACCATGATCATAAATGTATTTCATTGCATATAGATTAATAATAGCGGGTCCCTTATCATCTAAACTGCCACGACCAATAATTGATTCATTTGTTATAACTGGGACAAATGCGCTTGTACGTCATTGGCTTTCATCTCCTGCAGGAACAACGTCAAGGTGGGCTAGTATTCCAATAATCTTATCTCCATTGCCAAATTCAGCAAACCCATAACGATTTTTAGGATCTTTATAAATATTAAATCCAAAACTTTTGGCAAGATTTAATGCATAATTTAAGGCATTGTCAGTTTCTTTTCCAAAAGGAAATTCACTGCTAAAATCTTCTTCACTAATTGAAGGAATTGCACAAATATTTGCAATATTTTTAATCATTTCATCAAAGTCTTCTTTACTTTGCTTATAAGTAATAAATTTTTTCATTCTTACCTCTTATTAATTTTTTTAATATATTATACATTTTAATCCAAATAAAAAAAATAAATAAGGCGTAGCCTTATTATTTGTTTAATCCAACAGCAATACCTTTGTTTTTAAAAACGTAGTCATTATTATTGTTATATACAACATCAATTAATTCGGGATAATCAATAAAAGGATTTCTATTTCCTTGATGTTTATATACTCCATTGTTTCTATCAATGTCAAATTTTGAAACCTTATCTAAATTATTTCAAGCTAAAAATGTTTTTAAAAATATAGGAGTTATAGTATTTTTTCCATTATTTTCTTTAAATACACGAGTTGATTGATCACTTGATGTTATATTTAAATCGTTATAAGTTAAACAAAAGTATAAATATGCCCTAGCAACATCACCCTTAAATTCATTAATAGGTTCAGTAACAGGTCCACCATCTTCAACTGACAAACCAGTTTTTGTACCGTTTTTGGATATTTTGTTGCCTTGCAATTCCTTAACTGTTCCATAAGGGTAATTTGCATGCCATGCATTAACTAATTTATCGGTTGGTCAAACATGGTGAGCATCGGTTCTCATTGGAACAGACTTACTAAATCATGATTGTGGAACTAAGTGTTCTCTATTCATTCCATCGCCTTCATACTTGCCTTGATCTCTATATTTTCCATGTCAATTAGTAAAAACATCTTCTTTATCCGGAATTTCTTCATAGATATCTAAAACCGATCCATCTTTTTCGTAATATTTATCAACAAAAGCATCTTTATATGTATCAAATAAGTCTTTATATTTTGTTGAGTGATTTCTATGAGCTTTTTGCAATGCAAATAAGTTATCTCTTAGGTTTTGTCCACTTAAGCCATTTAAGGATTTATAAAAGTCATTTGAATTATCGTAAATTATCTTATTATCTAAGGGTTTAGCTTCGGGCTTCTTTTCAGGGGTTGTTCCGTGTTTTTGTGATGTCGCCAAATTTATAAAACTATTTAATCTGAATGTAATTTCTTTAATAGCATTCAAATCATCACCTTTAATTGCTTCTAATCCTTTCACATATTCTTTATTAAAATTAAATTTTTGGTTAAAATTAAATGATTTTGTATTTATTTTTGCCGCATGTTTTAAAGTTGAATTTAATTGCTTCTTTGCTTCGTTTAATGCTGCTTGGTTTGTTCTTTCTTCAATTGATTTTTTAAATTGATCTAATAATGATTTAGTTTTGTTTAATTCATCATCTTTAATTTCTTTATGTTTATTAATGATGTTTTCTATTGTGGTTTCACCTTTTGGTTTAACTTCAAATTTCTTTGTTTTTACACATCCAGCAGCAACTAATGGCGCTGAAATTAGCAAAGGTAAAATAGCACCCATAGTTAGGAATATTTTTTTGGTTTTTTTCATTATTTTATATATTTCGCTTTCCTTTTTTGTATTTAATAAAAATTTATCATATGAATTAAATAAGTCAATTCAAATTAATAATAATTTTCAAAAAAACAAATAAAAAAACACCTATAAGTGTTTTTTAATGTTTAACAAAGAGTTTGCAAAGAAAATCTTATTCAGCTTGTTGTTTAGCAAGTCTACGGCGAGCTCTGGCTTTTTCTAAAGCTAATTTATGTTCTCTTTTAGCTGCTTTTACCTTGTTAGCTAATCTAACGTCTTTTCTCATAATTTCTCCTTTGCTTTGTTCAAGCAATAATTATATCTATTTTTTCTTAATTTGTTTAATTTTTTGCATTATTGTTTCAAGATCTATTTTTTCAATTTCTTGTATTTTCTGACTAATGCTTTCTTTTGTTGCTATATTTTTTTTATTTTTTTGGTTGTCATTAACATTATTTGAAACATTTGGTAATTCAATATCTTCTGTTGATATTTGAAATAGTTCTTCTTGTTCTTCGTTATTTAAATGTTGAATATCTTGATTATAGGATTTTTTAATCAAAGCATGTTCAGCAGTCAATTCAATATTTTTATCAATATATCCTTGATATTCCTGTCTTATTTTTTCCTGTTCTATTTCATCAGCTTCAATTAATTCATTAATTTTTATTGGCGTAATAAAATACCCAGGGTTAGTAAATGAATAATCTAATTTAATAAGTTTACTTTCTTTAGTCTTATCTTTTAATATAAATTTTCAATCATATTCTGCCAAATTACCATTATTATCAGTGTATAACATTTTTAAATTGCTCAATGTAACAGCAAGTGATTGAAGACCTAAAATATTATTCTTTAGTGGCGTAAACAACATTTTTGGTGTATTACGTGTGGTCAATTCTAATTTGCTAGTATCCCAACATTTTATATAGCCGCCTTGCGTTAAATAAATTATATTGTTGTATTGTGAAACTGTTATAAAACCACTTACTTGAAGTTGAGGATGTTTATATAAGTTTAAAAGAAGTCTAATGCCTCTTGCTTTCAAAGAAATCCCAACCTTTAGTTCGTTTTCTGAAACTAAATGATACATACCATTATTTAGTAAAATAAATAGCATTTTTTCATCATTAGAAATTTGAGCATCTATTAATTCATCATTATCATCTTTAAACGACATGCAAGTTTTTGTTTTAAGAGAAGATTTATTCTCAAAATCTCTTAGATTAACTTTTTTGGCATATCCTAATTTTGATATTAAAATAAAGTTTGCATAACTATTGAATGACGTAATTTCTATAACTCTAATAATTTTCTCTGAACTTTCTAGAGCTACTATTGATGAAATATGTTGGCCAAGATCTTTTCAATTGCAATCTTTAAATAAATGGCAATCAATAATAAAATAATTTCCTAAATTTGTAAAGAATAATATCTTTGATAATGAGTTTACTTTATCAAAATAGAAAACATTATCTTCTTCTTTTAATTTGAATGTGCTTAATTCATTGCTAGTATAAACTTTTAAACTTATTTTTTTATAATATCCATCTTTGGTTATATATAAATAAAAATCTTCATTTTTTGTCAATAATTTATGATCAATTTCTTTATCAATTTTTTGATCTGAAATTTCCGTTAATCTTGGCTTTGAATATTGATTTTTTATTTCAAGCAATTGCTTTATTAAATATTGATTAAATTCTCATTTATCATTTAATAATTTATTGCAATTTTCAATTTGAATTTCAAGGTTTTTCTTTTCTTCTTGAAATTCAATTTGATCCATTCTTGAAAGCTTATACAATCTTAATTCAGCAATCGCTGTTGCTTGCAATTCACTAAATTTAAATCTATTCATCAATGCAAGGATAACACCTTTTTTTGAGTTATCACTTTCTTTAATCAAATGTATAACTTCATCAGAAATTTCAGCTACTTTAATGAATCCTTCAACTATTTCTAGTCTTAATTTAAACTTCTTTAAATCATAATTAATACCATTTATATTAACTTCTTTTAAATGACTTAAATAAGCAATTAAAGCACTATAGAGATTTAAAATTACTGGGCGGTTATTGTCAATTGCAACCATGTTATAACTATATGAGATACTTAGTTCGGTTTTATTCATTAAGTATGCAATTATTGCGTCAGCATTTGCACCGTCTTCTAATTGAATAAATATTGAAATTCCGTTTCTATCTGTTTGGTCTAAAACTTCTTTAATACCAGAAATTTTTTTATCTATTGCAATCGCATCAATGTCGGCAACTAATTTTGATTTAACTACGCCAAAAGGAATTTCGATTATTTCAATACCAATAATTTTCGATTCATCTTTTTTATCATAAACATAATGATATTTCGAAGAAATTGTAATTCTACCTTGCCCACTTGAAAGAGCTTTTTTTATTTCATTTATACCATTAATAATTGCTCCTGTTGGGAAATCTGGGCCTTTAACTATTTCACTTAATTCTTCAATTGATATTGTAGGATTTTTGATTAATGCAATTGTTGCATCAATAACTTCACCTAGATTATGTGGTGGGATTTCTGTAGCAAAGCCAGCAGCAATTCCCTTAGCACCGTTAACTAATAAATTAGGAAATAAGGCCGGCAAAACAATTGGTTCGTATTCAGAGTCATCAAAGTTTGGAGCCATTTTTACAACTTTTCTGTCTAAATCTCTCAACATCAATTCACTAATTTTTTCAAGTCTTGATTCAGTGTAACGCATTGCAGCGGCAGGGTCATCATCAATTGAACCTTTATTACCATGCATTTCAATTAATGGGAAATTGCTTTTTCAATCTTGAGCCATTCTGACTAATGCCTCGTATATTGAACTATCTCCATGAGGGTGATAACGTCCGATAACATCCCCAACGATTCTAGCTGATTTTTTAAAAGGTTCGCTATTTTTTAAATGTAAATTTCACATTGAATATAAAATCCGACGTTGAACAGGTTTTAGTCCATCACGAGCATCAGGAATTGCTCTTTGTTGAATAATGTATTTTGAATAACGTCCGAATCTATCAGACATTATATCGGCCATATTTTTTTCAATAATGTTTTCAGTAACTTCTTGTATTTCTTCTTTTCTATCTTTTTTCATACGACCTACTTATTATTTATTTTAAAATCATCTTCTGATGAAAAATCAACATTATTGTTTATTCATTGTTTTCTTGCTATTACGTCGTCACTCATCAATGTTGAAACATTTCTTTCAACTAAAATTTCATCTTCTACATCAACTTTAATTATTGAACGAGTTTCGGGGTTCATTGTTGTTTCTCAAAGTTGATCAGCATTCATCTCACCAAGCCCTTTGTAGCGTTGAATTTCATAGTTGGTATATTTTGAAGTAATTTCTTTTAATTGATCTTCATCCCAAGCATAAAGGGGTTGTGTATTTTTTGATTTTATTGAAATCTTATAAAGAGGTGGCAAGGCAATATAGACCATACCATTTTCAACTAGTTTTTTCATATAACGTCAAAAGAATGTTAATAGTAATGTTTGAATGTGAGCACCATCAGTATCAGCATCTGTCATAATAATAATTTTGTGATATTGAGCATTTTTTAAATTGAAGTCTTCACCTATACCTGCTCCGATTGTATTAATTATTGTTGCAATTTCTTCATTTGCTAAAACATCGCTTAATTTTGCTTTATCGGTGTTGATAACTTTACCTCTAAGTGGCAATATTGCCTGAGTAACTCTATTTCTTCCTAATTTAGCAGAACCACCAGCTGAATCACCTTCAACTAAAAACAATTCTTTAATGGCAGGATTTTTACTTTGAGCAGGAGTTAATTTTCCTGAAAGAATCTTTCTATTCTCAAATTTATTTTTAATCTTACGTGCTTCGTTTCTTGCGTTTCTAGCTGCAACTTTTGCTTCGTATGCTTTTTTAATTTTAGAAATAATTTTTTCTGCTTCAATTTTGTTTTCATTTAAAAAGAAATAAAACTTTTGACTTACAAAATCTTCAACAACTTCTCTAGCCTCTGGCGTACCTAATTTATCTTTCGTTTGACCTACAAATTCCAATATTTCTTCTGGGACTTTTAAAGAAATAACAGCAATTATTCCTTCTCTAACATCCGATCCTTCAAAAGTTATTTTTTTAGCTAAACCATTTTTAGAAGCATAATCATTTATTGTCTTAGTTAATGCGGTTTTAAGTGCTGTTTCATGTGTTCCACCATCACGTGTCTTTACATTATTTACAAAACTGTAAATTGATTCACTATATCCATCAGTATATACTAATGCCAAGTCAATTTCAATGCCGTAATTGCACATACCAGATGCATAAAAAATTTCACTAATAAATTTTTTGCCTTCACCAACAAAATTTATATATTCTTTTATGCCATCTTTGGTTTTAAATGTTACATGTTCATCGGTATTTTCATTATAAAATTCAATAGTAAGATTTGGCATTAAAAAACTTGCTTCTCTTAATTTTTCTTTAATTATTTCAGGTGAATATTTTGATTTTTTAAATATTGAAAAGTCGGCTCAAAATTGAACCTTTGTTCCTTGTTTTGTAGTTGGAGATATCTCATGTGTTTTTTCAATAATTGTATCTTGCTGAAAGGCTGTAAAGTATGCTTTTTTATCTCTTCAAACCGTAACTTCAACTTTAGAACTTAAGGCATTGACAACCGAGGAACCTACTCCGTGTAATCCCCCGCTTGTTTTATAAGCACCATCGCCAAATTTACCACCAGCATGAAGTTCAGTAAAGACAAGCTCAACTCCACTTTTTCCAGACTCGTGTTTATTGACAGGAATTCCCCTACCGTTGTCTTCAACAATAACAGAAGAATCTTTCTTTAAAGTTACAATTATTTTATTTGCAAACCCACTCAACGCTTCATCAATAGCATTATCAAAAATTTCCCAAATTAAGTGATGAAGCCCACTTGAATCAGTAGACCCAATATACATTCCCGGTCTTTTTCTGACAGCATCTAATCCTTTTAAAACTTTTAAATCGCTAGCTTCGTAATTATTTAAACTCATATCTAAATTATAAAGAAAAAAAATCTCTTGAACTATTTAAAGTAATAAGAGACTTATTTTAGAAAAATTTTGCTTGCGATTTTTATAATTTGTGGATTTGAAAGATTAAAGTTTCTAATGTCTTTAAAATCAAAAAAAGCAATATACAAATCAAAACTATTGTCATCATTAATTGCAAAAATTAATTTAGACCCAATTGGATATTTTTCTAATTGTTTAAGATCTGGCATTTTGACGTCTAACATTGCTAAGGTCGTAGCAATGTTAGTATCGTGGCCTACAATCAATGTTAAATCTTTGCCGTTGTTGAATTCTTTTTTAATTAATTTATATATATTTTTTTTATTTGAATTTATAACATTTCTGTCGCAAAAAGCAAAGTCAATTACATAATCCTTAGCCTTACCTATTAATCTTAAATCTCTTATAAAATTATTAGATTTAAATATTGAATCATACGGTTCGTTGAAGTAGTATTTAACTACTAATAAATCACTTAAAGAACAAGCCATAAAGAACTTGCCTCTTCAAAAAACTCCATCTTTATTTTCATTCAATGTAATATTGGTTTCTTCATTTGTAAATAAACAATTTTTTAGGTTAAAAACGTCAATAATTTTTTGATATATTTTTTTACTTTTTGCTTCATATCTTCATAGCAAATCATAATCAACGAGATTAATATCGTCAGTGTTAATGTCATATCATTCATTCTCATTTTTGAATGAATAATCTTGACATTGAATGTCAACCTTTTTAGCAGGAAAAAGGCCAATTGAAAGTAACTGCGCAGTTTGAAAAGTTCTTTGCGTTGAATTTGCCATCACCTTAACATCAATATCATCTGTTATATTGAAAAATTCTCTAATTTTTTGAGCCATTTTTAGCTCAAAAAGTGCTCCTTTATTTGTTAAAAATGCACTTCCTAAATTATCAATGTTTCAATTTACAATATCCTTATTGAAATATTTAATTGAACGTTCTTTTGTAAAAAATGGATATCTTAAACCATGTCTACTTAGAATTAATAGTTTCACAATGTCACCTCATTTTGTCAATAATTACGAGTAATTATTTTTTACATAATTGCATAATCTTATATAATTATATTGTTATATAAATAATTTTTAATTATATAAATTTTAAGGAGAAAAGATGAAAAAAACTAGAATTGCAATTAACGGTTTTGGTAGAATTGGTCGTCTAATTTTTAGAGAAATTTTTAATGACCCTGAATTTGAAATCGTTGCTATTAATGACTTAACAGATGCAGCTACATTGGCTCACTTATTGAAATACGATACAGCTCATGGAAAATTGAATGAAAATATTTCACACACAGAAAATTCTATTGTTGTAAATGGCAAAGAATATCGTGTTTATAGTGAAAAAGATCCATTAAACCTACCTTGAAAAGAACTAAACGTAGATATAGTTATTGAAGGAACTGGAAGATTTGTAACAAAAGAAGGTGCTGAATTGCACATTCAAGCAGGTGCTAAAAAGGTATTTATTACTGCTCCAGCTAAAAGCGAAGGTGTTAAAACAGTTGTTTATTCAGTAAACGAAGATATCATTACGCCAGAAGATAAAATTTTATCAGGTGCTTCATGTACTACTAACTGTTTAGCTCCTATTGCCAACGTATTGGAAAAAAACTTTGGTATTGAAAAAGGATTTATGACAACAGTTCACTCATATACAGCAGACCAAAGATTACAAGATGCTCCACACAAAGATTTAAGAAGAGCAAGAGCAGCTGCTTCAAACATGGTTCCAACAACAACTGGTGCTGCAATAGCAATTGGAAAGGTTATTCCTTCATTGGATAAAAAACTAAATGGACTATCTCTAAGAGTTCCAACAATAACTGGATCATTCGTTGATTTAACTGTTGAATTAAAGAAAGATGCTACTGTTCAAGAAATTAATGAAGCAATGAAGAAATCTGCTTCTGAATCATTAGAATATTCTACAGAACCAATTGTATCAAGTGATATTATTGGTGCAAAAGCAGGCTCAATATTTGACTCACTTTTAACAAGTGTGTTAGAAGTAGAAGGCAAAAAACTATACAAAGTTTATGCATGATACGACAATGAATCTTCATTCGTTGCTCAATATGTTAGAACATTAAGACATATTGGAAAATTAACAAAATAGTTAAAAAAATATTGGATAAGAAATGAAACTTTCAATAAAAGAAATAATTAAAAAAATTAAATTACTTGAATTGGATCTTGAAGATCTAAAAAAAGAAGAATCCAAGGAAAACTTCATTGTTTATTTAGAAAACAAAAGACCAACGAACATTGAATATGATTATCAAGAATATTCAAACAAAATTAAAAATTTATATAATGAGATTTTTAAGTTAAGAACATTGGTTCAAGTAACTAATATAAACACAATAACATCATATAAAAAACATTCTATATCAGAATTAATTATTTTATTAGCTCAAAAAAGTAATCAATTAGAAAGATTGCAAGAAATGCGCGATTGCAAAAAAATTTCTAGGGTTACTACAAACGATGGCCAAAACGAATATACTGAATATCTTTTTGACCCTAAAATGGTTGCAATTGAACTTAGAAAATTAAACAAAGAAATTTCTGAAATTCAAATTGCAATTGATAGCGCAAACATTAATACATTAGTTGAAGTTAAGTAATTAACTTTCCTTAATAAATTTGCATTATTTTCTATAATTTTTTGTTTATTGTTATAGATTTCTATATTATTTACCTTCGTTTTAAGTTTGTGTTTATATGATTTTGCATTTGCATACTTAAACTAGCAAATTTATTTAAAAACTTTCACATTTAGTAAGGGTTAGAAAATGTAAACCGCCATTTTAGGCGGTTTTTTATTTATATTATTTATAAATATTAGTAATTAATAATATAATTAGATAATTATGAATACAACAAAAGTTTACATAGATTTTGAAGCAATTACCAATCCTTTTGCAAGATTAGTAAATTTGCCAAGTGGAACTCCTTATGCCTATACATTAGGTCTTTTAAATATTCACGGCCAATATCAAATTAAAACATTTATTATCGATTTTAAAGAACATCATAATTTAAATTCAATTTGAACTGCTATAAAAAATGCAATAGTTTCTGACATATTAGAGATCAATAATACTGTGAATTTAAAGGAAGTTGTATTTGTAGGTCATAATCCTGTTTTAGAAAATAAATGCTTAAAAAAATTATTTCCTGAAAATAATGTTGAACCTTTAATTTCCAATACAATTGTGTCATTGTCAAAATTAACAGGGAAAATTTTTAACATTCCATATTTTAATAAAATAAAAAAAATTATCGCTGATTCTGGCGATAGCGAACTTAATATGAGAATTAAAGATAGAAATGGTGTAATTGCTGCATTTGCTGGATATTGATTATATACAAAATCAATTAAAAATTTACGAGCAAATGACAGAAGAAAAAGATTTTTAATTAAGATGAATAAAAATCTTTTGTTAAAAGAATTAAAACGTTATTCTCATGACGATGTCAACAAAATGCTTTATATTGTTTCTCATCCTGATGAAACAGAAACTTTACTAAAAGAACTTTTATACAAACGTGAACTAATAAAACAAATAAAAAATTTGGAACTTGATGACAATTTAACAATTAAAGAAATTAAAGAAAAAATTTGAACATTATAATTTCTAAAAATAAAGGATATTATGAAAAATAAGCTTATTGAAAAAAAAGGTAAGTTTTCAATTAAAAAATTTTTCCCTCAAAGCAAGTTTGATTGAAAAATTTATATTATAAAAACATTGCCAATAATAATTGGCGAAATTATATTTAATTTAAATGGGCTACTTGATAATTTCATGGTTAGCCATATTGATGGTGGAATTGATGCCTTGGCTTATGCTAATACATATACGGGAATAATTTATACAATTTTCTTTTCTATTCAAGCAATCGGGGCAATGTTCGTGGGTCAATATTATGGCCGTAAAGACTTTGATAAAGTAAAGCAGATAATGAATTTAAGAATTTGAATGAATCTAATTATTACCTTAAGTTTTGCAATTCCTGCTTGAACTTTAGCAAAGCAAATGGTTCACTTGATTGGTGGGCAAGGAATAAAACCTCAATCTGAATTAGCATCTACATATTATTTAATGTTTATTGCCATAAACTGAGTTTTAACTTCTTTTAATTTTAATACAAATATGTTGCTAAATGAAACCGGACATTCAAAATACGCTTTTGTTTCGGCATGTTTAACAATGCTAACAAATGCTTCAATTAATTCTATTTGTTTATATGGATTTCACAAACCTGCATATTATGCTGCTTTTGGTTCAATTATTGCTGCGGCAGTTTGCTTTACAAGTGATTCTTTATTTACATATTACAAAGAAAGAAAAATTTTTATTAATGTATTTAAATTGTTTCATATTACAAAACCAATTGCAAAGCAATTATTAAAAAGAATTCCCGCCATGTTAATTGCCATTGCTGGAATGTTAACTTTACCTACAAGAATGATTATTTGAAGCCATGCATATCCTGATAATTTATCAGGCGGTTCAGGAATTGGCGAAAAATGAATGGGAATTAATGCTGTTACAATACTTGGTTTAGTCGAAAGCCTTTCATCCGTCGCCTCGGCAATAACTTCTGTTTGTGCAAGCAATGTTTCATTTTTTGTTGCCACAAGATTAGGAGAATCTGATTTTGAAGGTGCAGATAAGCACGCCCATTCATTGCGGGGTTTTCATGCTTTGGCTGGATGTTGCATGTCTGTATTAATGATTGCGGTTGTTTTTGGAATTGCATATTCTCCTACTACAACAAAAGGTGTTGGTATTGGTGTCTCTCAAAGATTTTATGATGTGCAAAGAAAAGATTACATTATTGAAATATTAAATAAATCTGATATTTTAAAAAATAGTGATTTATTAAATTCACTTGGCAATGATTTTACATTCACAAAAGGTCAAAATTTGGCTTATGATCAATGGGTGGCAAGCGCAGTACTGGCAACTTCAAAAGAATTCAAAGAAACATTTTTGTTATGTAGTGCAACCTTTATTTTCTTTAACCCTATATGATGCTGATTTTATACAAGCGCCGCTTTGCCTGCTGCGGGCGGTAGAAATATAATTGGTTCTATTACAATATTAGCAACACAATGAGCATCATTTATATGATTAATTATTATTACTTATGGAATTGTTATTCCATTACATAAAACTTATACCGGATATAGTGATTTATCAATTCCTCTAGAACTTGCTTATTTTATGCTATTTGCATTTGACTTTATTAGATGATTGATGTTTGAAATAGTGGCATTGAAGACTGATTGAAAGAGAAATATTACAAATGAAATTGAAAAACAAGATCCAAACTTTCCAAGTAATATAGCAGAAGCAACAGCAACACACGATATTGTTCATAAACAATAAAAAGGAGATTTAAGATGTTATATGATTTAGTTTCACCATATAATTCAGGACATTTAAAGGTTAGTGATATTCATAGTATTTATTATGAAGAATCAGGAAATCCAAATGGTAAGCCAATTTTGTTTGTCCATGGTGGTCCAGGTGGAGGAACCAAACCTTCATGTCGTCAATTTTTTGACCCTGAATTTTATAGAATTATTTTATTTGATCAAAGAGGATGTGGACTTAGCATACCTAGCGCCGAAATTAAACAAAATACAACTCAAGATTTAATAGAAGATATTGAAAAATTACGGAAATTTTTAAACATTGACAAGTGAATATTATTCGGGGGGTCATGAGGTTCAACTTTAAGCTTGTTATATGCAATTAATTATCCTCAAAACGTTTCAGGAATAATTTTGCGAGGAATATTTTTAGGTAGGGATGAAGACAATTATTGATTGTATCAAAAAGGCGCTTCTTATTATTTTCCTGCTGAATATGAAGAATATTCAAACTTTATCCCTGAAAATGAAAGAGGCGATTTAATTAATGCTTATTATAAATATTTAAATTCAAGTGACCAAAATGTTGCAGAGCAAGCAGCATATCATTGGAGCAAATGAGAACTTGGATTGGTGACAATGAAAAAGTTACCTAATTTAGAAGAAATTTTATCCGATAAAAAAAGTAATTTAGAATTAGCAAGATTAGAAAATTATTATTTTGTTAATAAGATATTTTTAAATGATGATAATTTTATTTTAAATAACGCAAGTAAAATCAAAGAAATTCCAACGATATTAATTCATGGTCAATTTGATATGGATTGTCGCCCAGTTGGCGCATATTTATTGCATAAAGCCTTACCAAAATCAAAGCTTTGAATAATTCCAGAATCAGGTCATTCAGGTCGTGATGGACATATTAACGAAGCATTAATGCAAGCTACTGAATACTTTAAAACAATTAAATAAGCATTTGCTTATTTTATTTTACTAAAAGATTGTGAAACAATAAATACAATATTTCTTATAATTTAAAAAGACATTTAAGGAGAGAAATGAATTCAATAAATAACGTTGCTAAAAAATTAAATTTAAGCGAAAATCAAGTAAAAAATACATTATCTTTGTTATCTGAAGGGGCAACAGTACCATTTATTTCAAGATATAGGAAAAATATAACTGGTGGTTTAGACGAAGATCAAATTACAAAAATAAATGATTTATATGTGTACGACGTTGAATTAAATAAAAGAAAAGAAGCCATATTAAATATATTGGCCGAAAATAAATTATTAAATGATGAATTAAAGCAAAAAATTGAAGCTGCTGATACAAAACAAGCAGTTGAAAATATTTATGAACCATTTAAAATAGGTAAAAAAACAAAAGCATCAGATGCTATTGCTCTTGGTTTAAGTCCATTGGCTGATGAAATTTTAAATAATAATGATGACAACTATAATCTTTATTATGCTGCTAAGAAATATATTAGTGATAAATTAAAAACTGAAGATGAAGTCTTACAACAAACTAAATTTATCATTGCTCAAAATATTTCTCAAGACCCTTCTACAAGAGAATATGTAAAAAATCAATTATGAGATTATGGAGTTATTGAAACTAAATTAAAGAAGAATGCAATTGATGAAAATGAAACTTTCAAACAATATTATAATTTCTCTGAAAGAGTTAATAAAATTCCAAATCATAGAATTTTAGCAATATCAAGAGGGGAAGACAAAAAGATTCTTTCATATGATATTACTTATAATGAAAAGAAAATTATTTATGATCTAAATCAAAAGTATTTCAAATCAAAACGAACTGCCAAATGTATTCAAGAATGTATTTTAGATGCATTAGATAGACTAATTATGCCTTCGATAATTAGAGAAATTAAAACCGAATTATTTGAAAGGGCTGAGGCAGAAGCTATTAAAGTATTTGCTTCAAACGTTGAACAAATGTTATTGAGTCCAGCAACTAAAAATAAAAGAATTTTAGCAATAGACCCTGCTTATGTTAATGGATGTAAAATTGCAGCCATTGATCAAAATGGTAACTTTTTAAAAGAAGGAATAATTTATCCTCATAATCACAATCAAGAACAATGATTTGAGGCAATTAATACAATAAATAAATTTATTGATGAATACAAAATAGATTTAATTGTTATTGGCAATGGAACAGCAAGCAGAGAAACTGAATCGTTTATTGATAAATTATTGCAAAAAAGACTTGAAAAAAATCCTAATGAAAAAATTAAGTTTGTAATTGTAAGTGAAGTTGGCGCTTCTGTTTATTCAGCTTCAAAAATTGCCCAAGAAGAATTTCCACAATTACATGTTGAATATAGGTCAGCAATTCATATTGGTAGAAAGTTCCAAGATCCATTAAATGAATTAATTAAAATAGATCCTAAATCAATTGGAATAGGACAATATCAACATGATGTTAACCAAAAGGAATTATCAAAACAATTAACCGAAAAATTAAATAAAGTAGTTAACCTAGTTGGTGTTGATCTAAATACAGCAACAAAAGTGATTTTATCTTATATATCAGGATTAAGTAACACCATAGCAAGCAATATTGTAAACTATAGAGAAGAAAATGGTTCATTTAAATCACGTGAAGAACTTAAAAAAGTGAAAGGACTTGGGCCAAAAGCATACGAACAATCAGTTGGATTTTTAAGAATACATGATTCTAATAATTTTTATGACAAGACTAATATTCACCCCGAAAGTTATAAATTAGCAGATACCATTGTCAATAAGCTAAATATAGATTTAAATAATATTGACAAAGAAAAATTATTATCCGTTGACAAAGAACAACTTGCAAAAGAATTGAATTCTGATAAATACAGTATTCAACTTATTATAGAATCATTGATTGCACCCGAAAAAGATATTAGAGATGACAAACCAGGATTTTTAGTATCAGATAAAGTCTTAACTATTGATGATATAACTCAAGGTCAAATAATTAAGGGCCAAATTCAAAATGTTACCGATTTTGGTGCCTTTGCGTTTATTGGATTAAAACAAAACGTTTTGATCCATATAACCAATATGAAGAAGAAAGAAAATCAATTTATTAAACACCCTTTAGATGTATTAAATGTTGGTGATAATGTAAACATACAAATAATTTCCATTGATAAAGAACACAATAAAATTCAAGGAAAAATTATTTGAGATTAATTAATAATCAATATTGCAACATTGCTATTAAAAAAATAGCAATGTTTTTTGTTGTAATTTTGTAAAATTTATATATAAAAGTTATTTATTGCACTGGGGGAAATTATGGCTATTAATAATCAAGAACGTGACGAATTACATAAAAAAATTTGAGATATAGCAAACAAATTGCGCGGTTCAATTGATGGTTGAGATTTTAAACAATATGTTTTAGGAATTATGTTTTATAGATATATTTCTGAAAATATTGCATCATATGCAAACAAGCGCCAGATTCAAGCAGGAATCGAAGACTTTGATTATGCAACTTTATCAGATGAAGAAGCATTGACTGGCAAAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTGCCTTCTGAATTGTTTGTTAATGTTGTTAAAAATGCTACAACAAATAATTGTTTAAATGAAACGTTGGACAATATTTTTAAAAATATTGAATCATCAGCAAAAGGACAACAAAGTGAAAATGATTTTTCAGGTCTTTTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTACAAGAAATAGCAGCCATGAAATTAGGCAATTACCAAGATAATTCAATAGATGCCTTTGGCGATGCTTATGAATATTTGATGTCAATGTATGCATCAAATGCCGGAAAATCAGGGGGTGAATATTTTACTCCTCAAGAAGTTTCAGAATTACTTACTAAAATAGCAGTTTTTAATAAGAAAAAAGTTAATAGAGTATATGACCCTGCATGTGGATCGGGCTCGTTACTTTTACAAACAATCAAGGTTCTTGGCAAAGAAAACATCAAGGATGGATTTTATGGCCAAGAAGTCAATTTAACAACATACAACTTATGTAGAATAAATATGTTTTTGCATGATATAGGATTTGATAAATTCAATATTTATAACGGCGACACATTACTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTTATTGTTTCAAATCCTCCTTATTCAATTAAATGAGAAGGAGAAGATAACCCTTTATTAATAAATGACCAAAGATTTTCGCCAGCTGGAATATTAGCTCCAAAAAGTAAAGCTGATTTTGCTTTTATACTTCATAGCCTTTCTTGACTTGCAACTGATGGCGTAGCAGCAATAGTTTGCTTTCCAGGCATTATGTATCGCGGGGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAAGCAACTTGTTTTTTGGAACAAGTATTTCAACATGCATAATGGTATTGAAAAAATCAAAAATTGACAACAATATCTTATTTATTGACGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAACAAATTAACTAGTCAAAACATTAACAATATTATTGATTATTATGGCCAAAGAAAAGACATTTCTTATATTTCTAAATTAGCCAGTGTTGAAGAAATTAAGTCCAATTCATATAATTTATCAGTCAATAGTTACATTGAAAAACAAGACACTTCAGAAAAAATAGATATTAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAACATTGAGAAGCGAAATTGACAAAATAGTTGCTGAATTAGAGGAATAGTTATGAACAAAATAGAAAAGTTAATTAAGGAACTATGTCCTAATGGAGTTGAATATAAAAGGTTATGAGAAATTGTTGATTTTGACAAAAAATTTAAAGGAGTAGATAAAAACAAACAACAAAATATATTGTCGTTTAAGCATGTTTCTGCAAATGAACTCAAGCTCTATAAAAAAGAAGAAACGGGAAATGTAAAATTATTATCAACAGGCCAATATGATGGTTTTACCTCCTATGTTGAAAATGATAAAAACATTAATTATGGTGAGATTATTTCAATACCATCTGGTGGCGCGCCATTAATAAAATACTACAAAGGATATTTTATTGATAGTTTAAATATATTATTATCATCAAAAGATGTTAAAAAATATAGTCTCAAATTCTTATATTACTTTCTAATTGCTAACAAGGAAAGCATTGAACAAAATTTTAGGGGTTCCTCAATAAAACACCCTGATATGGAAAATATTATCGAGTTGCAAATTCCGCTTCCCCCTCTTGAAATTCAAAATGAAATTGTTAATATTTTAGACAAGTTCACTGAGTTGACCACCGAGTTGACCACCGAGTTGACCTATAGAGATAAACAATATAATTACTATAGAAATAAATTACTAGACTTTGATAATAATAAAGAATTATTAAACAAGATAATGAATAATCAACAATGTAATAATAGTATAGTTGAATATAAGAGGTTATGAGAAATTGTTGATTTTGACAAAAAATTTAAAGGAGTAGATAAAAACAAACAACAAAATATATTGTCATTTAAGCATGTTTCCGCAAATGAACTTAAGCTCTATAAAAAAGAAGAAACGGGAAACGTAAAATTATTATCAACAGGTCAATATGATGGTTTTACCTCCTATGTTGAAAATGATAAAAACATTAATTATGGTGAGATTATTTCAATACCATCTGGTGGCGTGCCATTAATAAAATACTACAAAGGATATTTTATTGATAGTTTAAATATATTATTATCATCAAAAGATGCTAAAAAATATAGTCTTAAATTCTTATATTACTTTTTAATTGCTAACAAGGAAAACATTGAACAAAATTTTAGGGGTTCCTCAATAAAACACCCTGATATGGAAAATATTATTGAATTGCAAATTCCACTCCCCCCTCTTGAAATCCAAAATAAAATAGTTGAAATCCTTGACAAACTTGAAACATATACCAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTTTGAAACAAAATTGTCAATATAGTTGAATATGATTTAATTAAAAACTTGTGTGAAATAAAGCGAGGAAAAGTTTACTCAAAAGAATTCATAAAATTAAATAAAGGTGAATACCCTGTTTATTCATCTCAAAGTTTAAACGATGGAATTTTAGGAAAAATTGACAAATATGATTTTGATGGTGAATATGTAACATGAACTACAGATGGGGCATATGCCGGGACAGTATTTTATAGAATTGGTAGATTTTCAATAACTAATGTGTGTGGAATATTGAATGTTCTAAACAAATCAAAATTAAATGTGAAATATTTAAGCACTTGCTTATCTATGCAGACAAAAAAATTTGTAAACAAGGCAAGCGGAAATCCTAAACTCATGAGTAATATTATGGAAAATATTGAAATCCCTATCCCTCACATTTCCATCCAAAATAAAATTGTTGAAATCCTTGACAAACTTGAAACATATACAAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGAAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTACATATTATTATTAAATGAACTTTATGAAAAGATAATTAACATTATTGAATATAAGAGAATGAACGAGGTAACTATTAATTTAAAAAAGGAAACATTAGAAAAAAACAAATTACTAAATAATGGCAAATATCAAGTAATTAATGGTGGCAAAGAAATATATGGGACATATAACCAATATAATAATGAAGGAAATACTATAACAATTGCAGCGCGTGGAGCTGCCGGTTTTGTAAATTATATGAATGATAAATTCTGAGCAGGTGGATTATGTTATCCGTATAGAAGCAAAAATGAAACATCTTTTTTAACTAAATATATCTACTATTGGCTTAAATATAATGAAGAAAAAATATCAAATGAATTAGTTGCAAAAGGTAGCATTCCGGCATTAAATAAAATTGATATTGATAATTTTTTCATTCCCATTCCTCATATTTCAATTCAAAATAAAATTGTTGAAATCCTAGATAAATTGCAAGCCTATACCAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGACCTACGTAAAAAGCAATACGAATACTACAGAGATAAATTATTGAATTTTAAAGAAAAGAATACTAAAATTTAATTAGTAGCAATATTAAATAAGGGTGTAAACTATGGAAAAATATAACATTATTGTTGAAAACGAGAATGATACGGTTGTTTCTGAATATAAGCCTCTAGCGAATAAAGCAAAAGATTATCAAACAGAAAGTGAACTTGAAAGTCAATTCATATCAATGCTTAAAAACCAAGGATATGAGTATTTGAACATTCATAATGAAGAAGATTTAATTAATAATTTAAGACAACAAATTGAATTGTTAAATAAAATAAATTTTTCAGATAATGAATGAAAAAAATTTTTTAATGAAAATATTGCAAATAAAAATTTTTCAATAAAAGATAAAAGTAGAATAATACAAGAGGATTATCGCCAACTTTTAACTAGAGATGATGGTAGCCATAAAAATATTATTTTAATTGATAAAGACAATATTCATAATAATAAACTTCAAGTTATAAATCAATATGAAGAAGAAACAGGCAACTATAAAAATAGATATGATGTTACTATATTGGTTAATGGTATTCCGTTGGTTCATATTGAATTAAAAAGAAGAGGGGTTGCTTTAAAAGAGGCTTTTAATCAAATCAATAGGTATCAAAGAGATTCGTTTTGAGCAAGTAATGGTTTATATGAATATGTGCAAGTGTTTGTTATTTCAAACGGTGCAAATACCAAATATTATTCAAATACAACAAGATGACAACATGTTAATAAAAAATCAGATATGATAGATTCCAAAACTTCTAAGAGTTTTGAATTTACACATTTTTGATCTGATTCAAAAAATAACCCTATTCATGATTTAATTGATTTCACAAGTACCTTTTTTTCAAAACATACGATTTTAAATATATTGACTAAATATTGCATATTTACATCTGAAGAAATGTTGCTAGTAATGAGGCCTTATCAAATTGTTGCTGCTGAAAAAATAATCAATAAAATAAATATTTCACACAATTATAAAAAATATGGAAGTATTGCAGCGGGTGGCTTTATTTGACATACTACAGGAAGTGGCAAGACTTTAACCTCTTTTAAAACCGCCAAATTAGCATCACAATTGCCTTATATAAATAAAGTTATTTTCGTTGTTGATAGAAGAGATTTGGACTATCAAACAATGAAGGAATATGACAAATTAGAAAAAGGCGCTGCTAATTCAAATCGTTCTACATCAATATTGCAAAGACAACTAGAATCTAATGACCCTAATAAAAAAATAATAATTACAACTATTCAAAAATTATCAATTTTTATAAAAAATAATAGAAATCATCCTGTTTTTGATAAAGAAGTTGTTTTAATATTTGATGAATGTCACCGCTCTCAATTTGGTTCAATGCATACAGCTGTTATTAAAAATTTTAAAAAATATTATTTATTTGGTTTTACCGGAACACCAATTTTTGCAGCTAATATCAATAAATCAAGTCCGGAAGTGATTAAAACAACTGAACAAGCTTTTGGCCAAAAATTACATACTTATACAATAATAAATGCAATAAATGATAGAAACGTTTTGCCATTTAAAATTGAATATATTAAAACAATTGATGTTGATAAATCACTAAACAATAAAGAGGATCAAAAAGTAAAAGATATTGATCGGGAAAGTGTTTTTTTAGATCAAAGAAGAATTAAACTAGTTGTTGAATACATATTGAAACATTTCGATCAAAAAACTAGGAGAAATTCCAATGTAATTTATAGCCATTCAATATCTGATATTTTAGATAAAACTATTAAAAGAAATGTGAGCGGTTTTAATTCTATAATGGCGGTTTCTTCAATAGAAATGGCTAAAAAGTATTATTTAGAATTTAAAAACCAGCTTGCTCAAGACCCTAAAAACAATCTAAAAATTGGTTTAATATATTCATATGGGGTTAATGGCGAAGAATCATATGGTGGTTTAATCGATGAAGAGGATAATGAAAATACATTGCAATTAAATAAGCCAGATAGAGATTTTTTGGATGATGCTATTAAAGATTATAACAATATGTTCAACACAAACTTTAATAGTGAAGGTGAACAATTTCAAATGTATTATAAAGATGTTTCTTTAAAACTAAAAAATAAAGATTTGGACATGCTGATTGTAGTAAACATGTTTTTAACTGGCTTTGACGCCCCAACATTAAATACATTATGAGTTGATAAAAAACTAAGAGAACATGGTTTAATTCAAGCTTTTTCAAGAACAAATAGAATTCTAAATGATAAAAAGGTATATGGAAATATTGTATGTTTTAGAAATTTAGAAAAAGAAACTGACCAAGCAATTTCAATATTTGGCGATGGTGAAGCTAGTGGAATTGTTAAAATCAAAAATTTCGTTGACTATTATTATGGCTATAATGATAGTCAGCAAGTGCATCACAATGGTTATTGCGATTATATTCAAAAATTAAATAATGAATTTCCTTTAAATAATTGAAATGTAATAAGCGAACAAAAAAATAAAGAATTTATTATATTGTTTGGTAAGGTATTAAAATTAAAAAATATTCTTGCTTCTTTTGATGAATTCATTGGTAAAGAAATTTTAAACGTTAGAGATTTTCAAAATTATTCAAGCAAATATTTGGATATAAAAGATGAATTTAAAAAGATATATAAAGAAGAAATTGCTTCAATTAACGATGATGTAATTTTTGAAACCGAATTATTAAAGACTTACGAAGTTAATATTGATTATATATTGCAAAAGGTAGAAGAGTTTAGAGGAAAGACAATTACTGAAATTAACAATTTAAGAAGTGAAATATCTTTAGCCTCTTCTTCATCGCCTGAATTAAGAAGCAAAAAACAATTAATTGAAAAATTCGTTTCAACAATTAATAATAATGAACTAGATATCCCAACTAAATTTCAAGAATTTATGGAACTAGAAAAGAAGAAGGAACTTGACAAAATTATTAGTGAAGAAAATTTAGATTTTGATAAAACCCATAAATTTATGGATAATTCGTTTGAGTTAGGAGAAATAAAAACTGCTGGTAGTGAGATTGGAGATTTATTGCCTAAAATGCCATTATTTGGTTCAAAAGGAAAAGAAAGATCTATTAAAAAGAAGTCAGTTTTAGAAAAATTTGAAAATTATTTCCAAAAATTTTATATTTAAATTCTAATGTTTTTGGTTTATAATATAGTTACGTTTTATATTGATATATAAAGGGTAGGCTAAATGCCTCCCTTTTTACTTAAAATAGATAAAAAAGGAATGAAAATGAGTGAAATAAAATTAACAGCAAAAGAAATTTACAAACAAATTTATAACCTTTCACAAATTAAAAAAATAGATCAAGCAGAGGTTGTTGAAGCTTTTAAAAATACAGTTACAAAGTTAATAACTGAAACTTATGACGAAGAAGCAGATCTTGAATTTATTTTCGATCAAGAAAATGACAATTTTGCAATTATTAATCATAATAAAGTTGTCGTTGGCGATCCAATAACAAGCGAAGAAAAAGATCGCTTAACAAGATGCATAGAAATACCATTAAGTGATGCTAAAAAAATAGATCCAAATGTTGAAATTGGAGATTCTCTTTCTCAAGAAATTAACTTTGAATATTTTAGTAAAAAAGACTATAACAAAATTCTTGCTAATTTCAGTCAAGACATTAAAAATTTAGAAAGAAAAATGACTTGTGAAAAATATGCAAGTGAAGTTGGAAATTCAACTAAGGCAAAAATTGTTTCTGTTAACAAAGGAAAAGTAAATCTTGAATTAAGAGACGGAACTTTGGCATTTATGCCTTCTAATTTTGTAAATCAAAGAATAATTTCAAAATTAAATCCAGGTGATTGAATTGATGTTGTAATTGAAGAAGTAAAAGAAGAAAACAGCAATGCTCAAATAATTGTTTCGTCAGTTGAATCAAGACTACTTCAAAAATTATTTGAAATTGAAATTCCTGAAATTTCACAAGGCTTAATTTCAATAGTTAATATTGCAAGAATTCCTGGAGAAAGAGCAAAGGTTTCTATTAAAAAATCTAATGATGCTCCTGAAACAATGGAAGAAATCGGCGCAATAATGGGACGTGATTCTGAAAGAATTTCAACAATATCTAGAAAACTTAATGGTGAAAAAATAGATGTAGTTTTATATTCAGATGATATAAAAGAATATATTAAAAATGCATTAAGTCCAGCAAAGGTTATTGATTTAATTGAAGTTCCATCAAAAAATTCTTATCCTTCATTTAATGTTATTGTTCCAACAATTCAACATACGCTTGCTATTGGAAAGAAAGGCCAAAATGTTTCTTTAGCTTCTGAATTAGTAAAAGCAAAATTAGATATTTTAAGCCAAGAACAAGCAGACGAAAGAGGCATTGAATACAATTTTGAAAATGGTAATATTACAAAAGAAGAAATTGAATTACTTGAAAGTGGTAAAAAACTTCATCACTCATTTAAACACCCAAGAACTATTGGCAATAAAAATGCTACAAACAAATTTAATACGTTTGAAAATAGTTTTAATATTGATGAATTTGATGAAGACCTTGCCGAAATGAGAAGAAAAGCTCAACAAAATGATAATATCTTTGAAAGACAAATGTTTTCAAATAGTCTAGATGAAGATTTAGAAAGAACTTTATCAGAACTAAATGACGACAAAGAAAATAATGTTGATGAAAATGACCTTGATCCTTATTCAAAATCTTTTGATGAATTAAATGAACAAAAAAATGATGAAGAAGAGGATTATGAAAAAATTACAGCAACAAAAATGAAAGATTTTAAACGTGATGAAGATTTAAGCAGTGGATTAGAAAACATTGATTTAAGTGATTTGGATGATGAAAAATGGTAAAGTCTATACAAAGAAAGTCAATTGTTGATAATAAAATATATGATATAGATTTATTGCTTAGATTTTCTAAAGATAAAAACAATTTAATTAGCTTTGATCCTTACAAAAAGAACCCCGGAAGAGGCGCATATTGTCTAAATGATAGTGAACAAATTGAAATTCTATTTAAAAGAAGATTATTAAATAAAGCATTTAAGCAGAATATAGATATTCAAAAATACAACGAAATAAGGAAAGAGGTGGACGAATGAGTAAACAAAATATCCAAAAACGAAAATCAAACGTTAGCTCATTAAAAGAGCAATTAACTGCTGGTGAAACAAAATTACAAGATGGCGTTTTTATATTTAATGGGCCATTGACAATTTCAGAATTCTCTCTTAAAATAAATAAAAACCCTACTGAAATATTGACCTATTATTTTAAAAAAGGAATAATCAAAACAATTAACACAACATTAAGCGAAGATGAAATAGCAGAACTTTGTTTTGAATTTAATTTAGATTTTAAAAAAGAAGAAAATGTTGATGCTCAAAATTTGTTTAATAAGCTTGAAATTGTTGAAGATCCAAATGATTTAGCTTCTAGACCCCCAATAGTAACAATTATGGGACATGTTGACCATGGTAAGACTACATTAATTGATAGAATAAGACATAGTAATGTTACTGAAACAGAATTCGGTGGTATTACTCAACACACCGGAGCATATCAAGTTGAATATCAAGGACGCAAAATTACATTTTTAGATACACCTGGTCATGAGGCTTTTACTGAAATGCGTTCAAGGGGAGCAAAAATTACTGATATAGTAGTCATTGTAGTTGCGGCAGATGATGGAGTTAAGCCCCAAACTATTGAAGCAATAGACCATGCAAAAGCTGCCAATGTGCCAATTATTGTTTTTGTTAATAAAATGGATAAGCAAGGAGCTGATATTGAAAGAATAAAAAGTGAACTTGCTGATAACAATGTTTTATGTGAAGAATGAGGCGGAAACTACCAATTTGTTTATGGATCAGCTTTGAAGAATACTAATATAAATGAATTACTTGAAGCAATATTTTTGCAAGCTGATTTACTTGATTTAAAGGCTAATATAAATAGAGATCCTATCGGAACAATTATTGAATCTAGAATTGACAAAGGACGTGGAGTTGTTTCGACTGTTATTGTTGAAAGTGGAACATTGTTACCTCGTGATTTCATTGTAGCTGGAGGGCAATATGGCAAGATTAGAAGTCTAACTTCTATTGATGGTAAGCCTATACAAAAAGCATTGCCTGGTACTCCATGTATAATTACTGGTTTAAGCAAAAACCCTCAATCGGGTGATAAATTTGTAGCTATTAGTGATGAAAAATTTGCTAAAAAATTAGCATCTGAAAAATCATATTTAGATAAATGTAAAGAATTAAATGAGAAAAATGCATTTTCTATACAAGATGGAATAAAAACTGTAAATGTCATTGTTAAGACAGATGTTCAAGGAACAGCAGAAGCAATTAAACAAACATTGCAAAAAATCAGAAATGATGAAATCGCAGTTAATGTTATAAGAGCAACCTCTGGCGAAGTAACAAAAGCAGATATTTTGCTTGCTGAAACTTCTAATGCAAGAATCTATTTATTCAATAATACAATAAATAACGAAATTAAAAAGGCTGCTGAATTAAAGAATATAAAAATCTTTAAACATAATATCATTTATAAAATGGTTGAAGAATTAGAAACTTTAATAACAACCTTAAAAGAACCTGTTTACGAAGAAAAATTAATTGGATCTGCAACCGTCCTACAACTATTCTTTTATTCAAAAATAGGTCAAATTGCAGGATGCATGATGCAAGAAGGCATTGTTAAGGAATTTAGTAAGGTCGAGGTATACAGACGCAATAAATTAATTTTTAAAGGACAAATTATTTCTCTAAAAAGAGGAAAAAATGATGTCAAATCTGTTGAAAAAGGTTTTGATTTTGGAACTCATGTCAAGGATTTTGATAAAATAGAAGTAGATGATGAATTAAAATTTTATGAGGATGTATTAGTCAATGAACACAATTAGTCACGAAAGAAAAACACAATTACTATTAGAATTAATTGGAAATAGTTTTTATGAATTAAAAGATTTTGATGTAACAAACATTTCAATAAATGATGTTATATTATCAAATGATGGTTCTCATGCCAAGATCTATGTAACTTTATTTAAAGATCAAAAAAGATACTTAGAAAAATTAACCACAATGACACCATTTATTCGTTCGGTTGTGGCAAAGAATTGAAAATATAAAGTTCTACCAGAATTAATTTTTGAAATTGATACTGTAGAAGAACACGCAGCTAAAATCGATAAAATTCTAAATAAGATTGAAGAAGAAAAAAATAATTAATTAGAAAGCAAAGGAAAAATATGACCAATAATTTTGACGCTATTGTAATTGGCGGTGGTCATGCTGGAATAGAAGCAACTTATGCTTTGGCAAAAAGAAATTTTAAAGTCGCATTGGTAACTTTAAACATTAATAGACTTGCTATGCTTCCTTGCAACCCCTCAATTGGCGGTTCTGCAAAGGGAATAATAACACGTGAAATTGATGCCCTTGGCGGAGTTCAAGGGTTTTTTGCTGATAATGCAATGATTCAAATTAAGATGTTAAATACATCTAAAGGCCCAGCAGTTTGATCATTAAGAGCCCAAATTGATAAAGAAAAGTATTCGGAAATAATTTTAAACGACATTAAAAAACAAAAAAATATTACATTGATTCAAGATGAAGCAAGCGATTTAATTGTTGAAGATGGAACTTGCATTGGAATTGAAACACTTGAACACGGTAAACTATATTCAAAAGTTGTTATTATGACAACCGGTGTTTACATGAATTCAAGAATTTTGCGCGGTGAAAATATAAAATATGATGGACCTGATGGAGAAAAGACAAGTTCAACACTTTCAAAAAATTTAAAAAAATACGGTTTTGAAATAATAAGATTAAAAACTGGAACACCTTGCAGAATTTATACAGATTCAATAGATTTTTCAAAAGTTGAAAAAGAAGTTTTAGAAAAAAACGAACTTTGTTTTTCAAGCCGTAGTAATAAAAAACTAGATGAACAAACTTGCTGTTATTTAACACACACAACCTTAAAGACAAAAGAAATAATCTTAAAAAACTTAAAACGTAGTTCATTATATTCTGGAATCATAATTGGAATTGGTCCACGTTATTGTCCAAGTGTTGAAGATAAAATTGTTAGATTTCAAGAAAAAGATACACATCATATTTTCTTTGAACCAGAAACAGCACGTGGTGACATCATGTACATTAATGGGTTGTCTACATCAATGCCTGAAGATGTTCAAGATGAAATGATTGCATCTATTCCAGGTCTTGAACATGCAAGAGTTCAAAAATATGGTTATGCAATTGAATATGATGCAATTAACCCTTTGAATTTATACAAATCTCTTGAAAGCAAAATTTTAAAAAATTTCTTTTCAGCAGGTCAACCAAATGGAACGAGTGGTTATGAAGAAGCAGCAGCTCAAGGATTAATAGCAGGAATTAACGCTGCTAATAAACTTGATAATTTAGAACCATTAATCATAAAAAGAAATGACGGTTATATTGGCGTTTTAATTGATGACATTGTAACTAAAGGCACAAACGAACCTTATAGAATGCTAACAAGTAGGGCAGAATTTAGACTTCTTTTGAGAAATGATAACGTTGATGAACGTTTGTATAAATATGCATATGACAACAAAATGATAAGCAAAGAAGAACATGATAGGATTGAAGAAAAATATAAGATTATTGCCGAAACAGTAGAAAGATTAAAGAATGAATATGTTTCTTCTAAAAGTGATGTTGGTCAAAAGTACAATGTAACAAATGGCATGTCATATTTAAAATTACTTTCAAATCCCGAAGTAGATCCAGTTGATATTTTAGGCTTAAATTTCCCATATATTGATGAAATAACTATTCAAGTTCGTTTGTATGGATATTTAAAGAAACAAGAATCAGCAGCGGATAAAATGCTTAGATTGGAACAATTAAAATTACCAAAAGACATTGATTATTGAAGCGTTGATAATTTAGCAACAGAAGCAAGACAAAAATTAGATAAAATAAGACCTGAAACAATTGGCCAAGCAGCAAGAATAAGTGGAATAAATCCTGCTGATATTCAAATGCTAATATTTTATTTAAACAATAAGCGTTAGTAATGAAATTAAATATTATAGCAGTAGGTTCTCTAAGCAAGGAATATAAAACTATCTATGATCAATATCTTAAAAAAATTAGTTTTTTTTGTTCAATAAATTTAATTGAAATAAAAGAACAAGTTAATAAAAATATTGATTTAAAAATAGCTGAAGAAACAAAATTAATCCTAGAAAAAATTCCTAAAAACTCCAAAGTTTATTATTTATCATTAAGAGGCAAGAAACTTGATAGTGGCGAATTTTCGGATTTACTTATTGAAGATAATATTACTTTTGTAATAGGCGGTAGCAATGGAGTTGATGAAACATATTTTAAAAATAAAATATGTTTTTCAGATTTGACATTTCCTCATCAATTATTTAGAATAATGTTAATTGAACAAATTTATCGAGGATTTAGTATAAAAAACAACATAAAATATCACAAATAAAATAGGCTTTTATTTTAAAAAACAAGTCTATTTTTTTATTTCTTATTATTTTATTTAAAAGGAATAAAACATTTATAATTTAAATGTAACCATGTTAAATTTAAAAAATAAATTCTTGAAAGGAGTTTACATGGCAAAAAATACATGAGAAATTGCCAAACAGGCAATTGAAGAACACGATAATATTTTTATATTTCATCATATTAGACCTGACGGAGATTGTTTAGGCTCTCAATTTGGTTTGAGAGAATTAATAAAGGAAAATTATCCTGAAAAAAACGTTTTTGTTTTAGGGGATAACGGAGGAATCTTTCCATTTTTAGAATGAGATTTTGATAATTTTGAAAATATTGATAAAAAATATCTTAAGAATTCCTTAGGGGTAGTAGTTGATGCTAACTGCTCAAATAGGATTCAATTTGCTAATTATATTTTAGATGGTAATTTTACCCATATGTTAAGAATAGATCACCATCCAGTTGATCCAGATATTAATTACGACTATACTTGAGAAGATGCAACATATGCAGCAGCTGCTGAACAAGTTGGATATATCGCAATGATGGCAAAATGAAGAGTTACAACAAAAGCTGCTAAATATGTTTATTTAGGCATAAACACAGACTCTAATCGTTTTCAATATGACTATGTTCAAAAAAGAACATTTGATGTAGTAAGTTATCTTCATGAAGGCAATGATTTTAAAGTTTGAGACATTAACTTCCCTCTTTCAATTCGTGAAGAAAAGAAGGTTAGATTCAATGCCTATGTTCTTTTACATTACAAAAATGAAGGCAAAGTACTTTGATTCTATGCAAATAAGAAAATTCAAAAAAAATTTTGTTTGAATGAAAAAGAAGCAAATGATGTTGGAATTTTAGCAAATATTGGTGATGCTAAAATTTGAGTGCTATTCATTGATGAACCAAATGGCAAAATTAGAGCGAGAGTAAGAAGCAATGGAATATGAATAAATCATATTTGTGCAAAACCTGAATATTACCCTGGTGGTGGTCACGAAGTTGCTTCAGGAGCAACTTGTGCTAATAAAAAATCTATGAAACATTTAATAGAAGACTTAAAGGCAGAGGTACTAAAATATGAATAAAATAAGTGCAGAAAAATTAGCAATTTTCAAAAAAATTGAAGAAAGAATTAAAGCACACAAAAATATAGTTATATATCATCATATTAGACCTGACGGAGATTGCTTGGGTTCTCAATTTGGAATGAAGAATTTAATTAAGGTAAATTTCCCTGATAAAACAGTGTATACAATTGGCGATTCCAAAGGTATTTATTCATTTTTAGAATTTAAAATGGATAAATTACCACTAGAAAAGTTAGATGATTCATTAGCAATTATTGTTGATGCTAACTTTAAAGAACGTTTAGAATGTAGAGAATATTTAGACAATAATTGTTTCGATGAAGTAATAAGAATCGATCACCATCCAAATGATGATGATTTGGATGCTTCATTAAGATGAGTTGAACCAGAAGCACCAGCTGCTGCTCAACAAGTAACTGAAATAGCATACGAATTAGGTTGAAAATTAAACAAAGAAGCTGCAACTTATTTATATTTGGGTATATATACTGATTCAGTTAAACTATCAACCAATACGACAACCGCAAAGACAATGTTGCTAGTAAGTTGATTGTGAGACAATGGCAGTCAAAAAGACTTAATTCATACAGAACTTTCTAAGCGTACATTGTTTGATATTAACATAAACACATACATATCTCAAAACATGAAAATTGCAAACGACGTCGTTTCATTTTACTTCCCATTGTCTGAACAAATTAAATTAGGAATTAAAGATCCATTAAAAGCAAATAGACCTTTTGTTCTTGGCTCAATTGATAACACAAAGGCATGAGTATTCTTTACAGAAGAAAAACCTGGCCAAATTCGTTGCGAATTTAGATCAAATGGTTGCAATGTAAGAAATGTAGCAATTAAATGAGGTGGGGGTGGACATATACGTGCATCAGGCGCTCAAATTTCTGATCCTAATTTAATTCCATTGATTATAAAAGACTTAGAACAAGAAACAAAAAATCTTGCTGATTATGAATAAAAAATAGAAATAATTTGTTAAAAAAGATGATTCTGTAAAATAAGAATCGTCTTTTTTAAAAACGACCCTGAACGCAAACAAGGCTATGAGGATTTATCTAAACTAATTGAAGAGATAAATAATGGATTAAATTCTTTAGATATGAACGATATAAAGAATTTAGATTGAGTAAATGCAAAAAACTCAGAATTAAATAGAAAACATAATCATTATTTAAAAGATACAACCTTCTTACAGAAGAAAAAAAATAAAAGTTAAAAACCAAGATTTGACAATACAACAAATCTTGGTTTTTAACTAGAATTAAATGTTAGTTATGAATAAAATTATCTAATATATAATTATCAACTTTCTTGTTTATTGATTTAATAAAAGAAAAATAAGTTTTGTATTTCTTAAAATCTCCCTTTCAAACAAGCTTTTTATTTAATGAATTTGCTAAATCAATTGCTAAATAGACATCAAAGATTCCAAATTCATTATGGCAGTCAATAATCATTTTTTCAATTAATGAATTTATTTCGTGGTCTGAGATATCAATATTATCTCTTTTATATGAGCTTAATGCTTTAGTAAATAAATATCCGAAGTATATGTTTCTACTTTGTTGATATTGTTTAAATTTTTCAATAAATTGCTTATGAATTTCATTTTCTTCTGAGCTTAGAGAAGCATTTGCAAAGAGCTTATCTGTATCGATTGCATTTTTTATATATACATTTTCTTTTTTTAATATTAATATCATTTTAATTACAATTAAAATGAATATAGCTAAGAAGATTGCACCAAAAACATAACAAACGATACTTGATTTTTTAGATATAGTTCCCATAATGCCATTTGCTGCTAAAAATAGCAAGATAGCAATAACGAGAAAGAGAGCAACGGTTAGAAAACCAATAATGTTTAATATAACATTTTTTATTTTCATTTTCTTTTCTTTTGATGTCATTTATTTAGCCTCCTTATAAGTTATTAGTTGAGGATTTTTTAAATATCCCCTTCCAGCTTTAGCGATATTATGGCCATTCAATCTTGTTGCATCGGCGCTAAAATTTACTCAAATTTTTAGCAGTGCTGCACCGATGCCGTATGTGTCAACAGGAGTCTCTTCGGCTTCAAATTCAGCAATTCTTTGAGCGTCAAATCCGCTTGAAACAGTTATTTTTACATGCTTGCCGTTATTATTGTCTAATGCTTCTCTTAACCTTTTTACTTGATTGGGTGTAACGCCAAATTCTGCTTCGCCTTCTTTGAACATTTTATCAATAAGCGCTTTTGATGTGTCTATTCTTACGCTTTTTAAGTCTTTGCCAAAAGCCTTTAAACATTTTAATGAATCGCTTATAACATCGTTGTGAAAATCAACTAGGGCAACTAGATCATCATTTGGAAAAACATTTTTATATGCTTTGCAAGCAGCTACAATATCACCGTCAAACATTTGAATTAATGCATGAGGCATGCTTCCAAAAGTATGCTTTGGATCAAAATTTGAGCTTAGAAAAGTCGAGTGATTTTCTATACCACCTATTCTTATTGCATATGAGTCGTTTGGCTGATTTATATAATGATCTGCCCTATCTGCCATGCTTACGATTTTTTTGCCATTTGCTGCTTTTATTGCTCAATATGCATTAGTTGCTATTGACGATTGCCTTGCTAAAATGCCATCAATGATTCCTTCTCATATACCAAATTCATAATAAGGGCCTTCAAGTTCTAAAACTACTTCTCTATTATGGATTATACTGCCTTCCGGCATGTATCTTATTGTGTATTTAGAAATATCTGTATTTTGTTTTAAAATATCAATTGCTTCATTTATCCCACATAATTTGACATCATTATGTCTTTGAAAGAATTGTAGAGTAATGTTTTGATCTTTTTTAAACTTCTTTGATATTTCTTGAGTCTTGTAAAAATAAATTGAAATGTAATCTTTCAAATTGATTTTTTTCATTATTCTCCTTTTTATAATAATTTAATAAATTATTATTAGCCTCTTATTAAAATTTTAAACAAAATTAATTAATAAAATCATATAAAGTTATAAAATAATAAATCATTGTTAAAATATCTTTTTTAAAGACATGTTTTATAATAAAGGAGAATAAGTATGGCAAAAAACCAAGATAAAAAAATGCTATCTCCAGAAGAAAAAGAAATAGAAAATATAAAGAAAATATCAAAAAATGGTTGACATTTTAGTTATTGGTCGTTATTTTTTATTTTAGGAGTTATAGCTATTTTAATATGAGCAGTTGAATCTAAAAGTTCATCTTCAATGGTAAAATATCCATTATCTATAATATTTATAATTCTTTGCATAGCTATGATAGCAGGAATGTATACAATAAATGTAATGGGTTATAAACTAGCAATCAAATTAAACAATGAATCTTTAAGAAAATGCTATTTGATTTCACTAATTCCATTATTGTTTTTTGTAGCATTGGTAGCAAGCTTTGTGTCTTCAATTAAGCTAAAAGAAATTTATATAGATAAAAAAAGCCAATTTCAAAATGTTGCTATAGCAGGATAGAAAAAAAACCTTGAATTAATTTTCAAGGTTTTTTTGTTTAATTTCTTTTGCTATTTTGTTTGCATTTCTTTGGCGATGAGTAAATAGAATTATATAAAATACTAATGTAGGAATTGCAATACTTATAAAAATTATAAAAAAGCAAGATAAGGCTATGTAACCATTTTGATTTCATAGGATAAAATAATTATCTAATGAAGTTATAGCATAAACACTTAATGTTGGAATGAACGCTATTGCAACTTGCGAAATTAAAGTGTGGCGTTTAGTTATTAATTGTGGTTTTTTCATCAATAGTATTGATGTTGTTAATATGTATGTAATTAAAATATAACCAACAATTACAAGTATTATAGGTAAGGTAATTTTCATAGTTAAAATTTTACTCCATTAAAAAATAAGAGCATTTAAAAAGCACATGTTTTAAACATGGCTTTTTAAAATTAGTTATT", "length": 672843, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_013367975.1|CP055147.1", "orig_description": "HMT-632 Metamycoplasma hominis SS25" } ], "run": { "start": "2026-01-28 09:29:18", "end": "2026-01-28 09:36:36", "duration": "7.30 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }